LOCUS       DAZKHK010000001        91139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKHK010000001 DAZKHK010000000
VERSION     DAZKHK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 91139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.001"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..536)
                     /locus_tag="ACHOS5_000001"
     CDS             complement(<1..536)
                     /locus_tag="ACHOS5_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9774900.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQ"
     gene            complement(794..2047)
                     /gene="amiC"
                     /locus_tag="ACHOS5_000002"
     CDS             complement(794..2047)
                     /gene="amiC"
                     /locus_tag="ACHOS5_000002"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP9774901.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2279..3610
                     /gene="argA"
                     /locus_tag="ACHOS5_000003"
     CDS             2279..3610
                     /gene="argA"
                     /locus_tag="ACHOS5_000003"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP9774902.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3672..5498)
                     /gene="recD"
                     /locus_tag="ACHOS5_000004"
     CDS             complement(3672..5498)
                     /gene="recD"
                     /locus_tag="ACHOS5_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP9774903.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTPERAGQLSRLTG
                     THVPAGTGTDAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPNLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5498..9040)
                     /gene="recB"
                     /locus_tag="ACHOS5_000005"
     CDS             complement(5498..9040)
                     /gene="recB"
                     /locus_tag="ACHOS5_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP9774904.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITTVLLAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(9033..11921)
                     /gene="ptrA"
                     /locus_tag="ACHOS5_000006"
     CDS             complement(9033..11921)
                     /gene="ptrA"
                     /locus_tag="ACHOS5_000006"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP9774905.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAIFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGIAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12097..15465)
                     /gene="recC"
                     /locus_tag="ACHOS5_000007"
     CDS             complement(12097..15465)
                     /gene="recC"
                     /locus_tag="ACHOS5_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP9774906.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIISEGL
                     GAQYGDAVPLSLLRDELALRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCHHTRMPFDPQNYQPGN
                     LQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15478..15801)
                     /gene="ppdC"
                     /locus_tag="ACHOS5_000008"
     CDS             complement(15478..15801)
                     /gene="ppdC"
                     /locus_tag="ACHOS5_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP9774907.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLHAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15786..16193)
                     /gene="ygdB"
                     /locus_tag="ACHOS5_000009"
     CDS             complement(15786..16193)
                     /gene="ygdB"
                     /locus_tag="ACHOS5_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP9774908.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIFFSPRGWSDFCPLKERALCQLP"
     gene            complement(16190..16753)
                     /gene="ppdB"
                     /locus_tag="ACHOS5_000010"
     CDS             complement(16190..16753)
                     /gene="ppdB"
                     /locus_tag="ACHOS5_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9774909.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16744..17214)
                     /gene="ppdA"
                     /locus_tag="ACHOS5_000011"
     CDS             complement(16744..17214)
                     /gene="ppdA"
                     /locus_tag="ACHOS5_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9774910.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17399..18193)
                     /gene="thyA"
                     /locus_tag="ACHOS5_000012"
     CDS             complement(17399..18193)
                     /gene="thyA"
                     /locus_tag="ACHOS5_000012"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9774911.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18200..19075)
                     /gene="lgt"
                     /locus_tag="ACHOS5_000013"
     CDS             complement(18200..19075)
                     /gene="lgt"
                     /locus_tag="ACHOS5_000013"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9774912.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEIENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19226..21472)
                     /gene="ptsP"
                     /locus_tag="ACHOS5_000014"
     CDS             complement(19226..21472)
                     /gene="ptsP"
                     /locus_tag="ACHOS5_000014"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9774913.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21485..22015)
                     /gene="rppH"
                     /locus_tag="ACHOS5_000015"
     CDS             complement(21485..22015)
                     /gene="rppH"
                     /locus_tag="ACHOS5_000015"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP9774914.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22368..22514)
                     /gene="ygdT"
                     /locus_tag="ACHOS5_000016"
     CDS             complement(22368..22514)
                     /gene="ygdT"
                     /locus_tag="ACHOS5_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP9774915.1"
                     /translation="MLSTESWDNCEKPSLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22700..23389
                     /gene="mutH"
                     /locus_tag="ACHOS5_000017"
     CDS             22700..23389
                     /gene="mutH"
                     /locus_tag="ACHOS5_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP9774916.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23458..24171
                     /gene="ygdQ"
                     /locus_tag="ACHOS5_000018"
     CDS             23458..24171
                     /gene="ygdQ"
                     /locus_tag="ACHOS5_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9774917.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24309..24527
                     /gene="ygdR"
                     /locus_tag="ACHOS5_000019"
     CDS             24309..24527
                     /gene="ygdR"
                     /locus_tag="ACHOS5_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP9774918.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24635..25675
                     /gene="tas"
                     /locus_tag="ACHOS5_000020"
     CDS             24635..25675
                     /gene="tas"
                     /locus_tag="ACHOS5_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP9774919.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQMKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25707..26900)
                     /gene="lplT"
                     /locus_tag="ACHOS5_000021"
     CDS             complement(25707..26900)
                     /gene="lplT"
                     /locus_tag="ACHOS5_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP9774920.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALVACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26893..29052)
                     /gene="aas"
                     /locus_tag="ACHOS5_000022"
     CDS             complement(26893..29052)
                     /gene="aas"
                     /locus_tag="ACHOS5_000022"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP9774921.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTTDKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENIRGEMERDWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29638..30669
                     /gene="galR"
                     /locus_tag="ACHOS5_000023"
     CDS             29638..30669
                     /gene="galR"
                     /locus_tag="ACHOS5_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP9774922.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30676..31938)
                     /gene="lysA"
                     /locus_tag="ACHOS5_000024"
     CDS             complement(30676..31938)
                     /gene="lysA"
                     /locus_tag="ACHOS5_000024"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9774923.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTYPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAAEGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32060..32995
                     /gene="lysR"
                     /locus_tag="ACHOS5_000025"
     CDS             32060..32995
                     /gene="lysR"
                     /locus_tag="ACHOS5_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP9774924.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32982..33674)
                     /gene="ygeA"
                     /locus_tag="ACHOS5_000026"
     CDS             complement(32982..33674)
                     /gene="ygeA"
                     /locus_tag="ACHOS5_000026"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP9774925.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAIIRAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33803..35221)
                     /gene="araE"
                     /locus_tag="ACHOS5_000027"
     CDS             complement(33803..35221)
                     /gene="araE"
                     /locus_tag="ACHOS5_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP9774926.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35536..36297)
                     /gene="kduD"
                     /locus_tag="ACHOS5_000028"
     CDS             complement(35536..36297)
                     /gene="kduD"
                     /locus_tag="ACHOS5_000028"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP9774927.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36327..37163)
                     /gene="kduI"
                     /locus_tag="ACHOS5_000029"
     CDS             complement(36327..37163)
                     /gene="kduI"
                     /locus_tag="ACHOS5_000029"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9774928.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37450..38631)
                     /gene="yqeF"
                     /locus_tag="ACHOS5_000030"
     CDS             complement(37450..38631)
                     /gene="yqeF"
                     /locus_tag="ACHOS5_000030"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP9774929.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38886..40115
                     /gene="yqeG"
                     /locus_tag="ACHOS5_000031"
     CDS             38886..40115
                     /gene="yqeG"
                     /locus_tag="ACHOS5_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9774930.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            complement(40431..40504)
                     /locus_tag="ACHOS5_000032"
     tRNA            complement(40431..40504)
                     /locus_tag="ACHOS5_000032"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40470..40472),aa:Gly,seq:ccc)
     gene            complement(40583..41338)
                     /gene="actS"
                     /locus_tag="ACHOS5_000033"
     CDS             complement(40583..41338)
                     /gene="actS"
                     /locus_tag="ACHOS5_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP9774931.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            41752..44049
                     /gene="xdhA"
                     /locus_tag="ACHOS5_000034"
     CDS             41752..44049
                     /gene="xdhA"
                     /locus_tag="ACHOS5_000034"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP9774932.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVHVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            44060..44938
                     /gene="xdhB"
                     /locus_tag="ACHOS5_000035"
     CDS             44060..44938
                     /gene="xdhB"
                     /locus_tag="ACHOS5_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP9774933.1"
                     /translation="MFDFASYHRATTLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLELHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            44935..45414
                     /gene="xdhC"
                     /locus_tag="ACHOS5_000036"
     CDS             44935..45414
                     /gene="xdhC"
                     /locus_tag="ACHOS5_000036"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP9774934.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(45454..47232)
                     /gene="ygeV"
                     /locus_tag="ACHOS5_000037"
     CDS             complement(45454..47232)
                     /gene="ygeV"
                     /locus_tag="ACHOS5_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9774935.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDADNRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            47711..48898
                     /gene="ygeW"
                     /locus_tag="ACHOS5_000038"
     CDS             47711..48898
                     /gene="ygeW"
                     /locus_tag="ACHOS5_000038"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP9774936.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            48956..50152
                     /gene="dpaL"
                     /locus_tag="ACHOS5_000039"
     CDS             48956..50152
                     /gene="dpaL"
                     /locus_tag="ACHOS5_000039"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP9774937.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            50210..51421
                     /gene="ygeY"
                     /locus_tag="ACHOS5_000040"
     CDS             50210..51421
                     /gene="ygeY"
                     /locus_tag="ACHOS5_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP9774938.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            51474..52859
                     /gene="hyuA"
                     /locus_tag="ACHOS5_000041"
     CDS             51474..52859
                     /gene="hyuA"
                     /locus_tag="ACHOS5_000041"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP9774939.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGILAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            52907..53839
                     /gene="arcC"
                     /locus_tag="ACHOS5_000042"
     CDS             52907..53839
                     /gene="arcC"
                     /locus_tag="ACHOS5_000042"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9774940.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(53880..55505)
                     /gene="yqeB"
                     /locus_tag="ACHOS5_000043"
     CDS             complement(53880..55505)
                     /gene="yqeB"
                     /locus_tag="ACHOS5_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP9774941.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(55553..56323)
                     /gene="yqeC"
                     /locus_tag="ACHOS5_000044"
     CDS             complement(55553..56323)
                     /gene="yqeC"
                     /locus_tag="ACHOS5_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP9774942.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHCWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPAAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            56427..57005
                     /gene="mocA"
                     /locus_tag="ACHOS5_000045"
     CDS             56427..57005
                     /gene="mocA"
                     /locus_tag="ACHOS5_000045"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP9774943.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            57327..60425
                     /gene="ygfK"
                     /locus_tag="ACHOS5_000046"
     CDS             57327..60425
                     /gene="ygfK"
                     /locus_tag="ACHOS5_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP9774944.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQNEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLSDVFMIGDVQRGPSSIVAAVGTARRATDTILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            60428..61756
                     /gene="ssnA"
                     /locus_tag="ACHOS5_000047"
     CDS             60428..61756
                     /gene="ssnA"
                     /locus_tag="ACHOS5_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP9774945.1"
                     /translation="MLILKNVTAVQLHPAKVQESVDIAIENDVIVAIDDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     SGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALANGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            61807..62586
                     /gene="ygfM"
                     /locus_tag="ACHOS5_000048"
     CDS             61807..62586
                     /gene="ygfM"
                     /locus_tag="ACHOS5_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP9774946.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQSLRDARFIPAALREALGFVYSRHIRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGIRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            62583..65453
                     /gene="xdhD"
                     /locus_tag="ACHOS5_000049"
     CDS             62583..65453
                     /gene="xdhD"
                     /locus_tag="ACHOS5_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP9774947.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PTAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNR
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            65618..67018
                     /gene="xanQ"
                     /locus_tag="ACHOS5_000050"
     CDS             65618..67018
                     /gene="xanQ"
                     /locus_tag="ACHOS5_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP9774948.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            67036..68352
                     /gene="guaD"
                     /locus_tag="ACHOS5_000051"
     CDS             67036..68352
                     /gene="guaD"
                     /locus_tag="ACHOS5_000051"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP9774949.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            68388..69755
                     /gene="ghxQ"
                     /locus_tag="ACHOS5_000052"
                     /gene_synonym="ygfQ"
     CDS             68388..69755
                     /gene="ghxQ"
                     /locus_tag="ACHOS5_000052"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP9774950.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(69791..70279)
                     /gene="ygfS"
                     /locus_tag="ACHOS5_000053"
     CDS             complement(69791..70279)
                     /gene="ygfS"
                     /locus_tag="ACHOS5_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9774951.1"
                     /translation="MKSLIIVNPAACIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRIRQQRIAASGAN
                     PL"
     gene            complement(70279..72198)
                     /gene="ygfT"
                     /locus_tag="ACHOS5_000054"
     CDS             complement(70279..72198)
                     /gene="ygfT"
                     /locus_tag="ACHOS5_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP9774952.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKMARQRKTAAGKASS
                     DAQLSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            72634..74082
                     /gene="uacT"
                     /locus_tag="ACHOS5_000055"
     CDS             72634..74082
                     /gene="uacT"
                     /locus_tag="ACHOS5_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP9774953.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            74084..74209
                     /gene="yqfG"
                     /locus_tag="ACHOS5_000056"
     CDS             74084..74209
                     /gene="yqfG"
                     /locus_tag="ACHOS5_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP9774954.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(74206..74277)
                     /locus_tag="ACHOS5_000057"
     CDS             complement(74206..74277)
                     /locus_tag="ACHOS5_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9774955.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            74332..74880
                     /gene="idi"
                     /locus_tag="ACHOS5_000058"
     CDS             74332..74880
                     /gene="idi"
                     /locus_tag="ACHOS5_000058"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9774956.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQPGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(74923..76440)
                     /gene="lysS"
                     /locus_tag="ACHOS5_000059"
     CDS             complement(74923..76440)
                     /gene="lysS"
                     /locus_tag="ACHOS5_000059"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9774957.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(76450..77548)
                     /gene="prfB"
                     /locus_tag="ACHOS5_000060"
     CDS             complement(join(76450..77472,77474..77548))
                     /gene="prfB"
                     /locus_tag="ACHOS5_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9774958.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(77639..79372)
                     /gene="recJ"
                     /locus_tag="ACHOS5_000061"
     CDS             complement(77639..79372)
                     /gene="recJ"
                     /locus_tag="ACHOS5_000061"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9774959.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILCNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEEKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(79378..80088)
                     /gene="dsbC"
                     /locus_tag="ACHOS5_000062"
     CDS             complement(79378..80088)
                     /gene="dsbC"
                     /locus_tag="ACHOS5_000062"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP9774960.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(80113..81009)
                     /gene="xerD"
                     /locus_tag="ACHOS5_000063"
     CDS             complement(80113..81009)
                     /gene="xerD"
                     /locus_tag="ACHOS5_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9774961.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            81121..81642
                     /gene="fldB"
                     /locus_tag="ACHOS5_000064"
     CDS             81121..81642
                     /gene="fldB"
                     /locus_tag="ACHOS5_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP9774962.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(81682..82089)
                     /gene="ygfX"
                     /locus_tag="ACHOS5_000065"
     CDS             complement(81682..82089)
                     /gene="ygfX"
                     /locus_tag="ACHOS5_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP9774963.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(82070..82336)
                     /gene="sdhE"
                     /locus_tag="ACHOS5_000066"
     CDS             complement(82070..82336)
                     /gene="sdhE"
                     /locus_tag="ACHOS5_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP9774964.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            82579..83559
                     /gene="ygfZ"
                     /locus_tag="ACHOS5_000067"
     CDS             82579..83559
                     /gene="ygfZ"
                     /locus_tag="ACHOS5_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP9774965.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRMRDDANTLRIEPLPYSLEE"
     gene            complement(83636..84295)
                     /gene="yqfA"
                     /locus_tag="ACHOS5_000068"
     CDS             complement(83636..84295)
                     /gene="yqfA"
                     /locus_tag="ACHOS5_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9774966.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(84459..84770)
                     /gene="yqfB"
                     /locus_tag="ACHOS5_000069"
     CDS             complement(84459..84770)
                     /gene="yqfB"
                     /locus_tag="ACHOS5_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP9774967.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMPLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            84815..86248
                     /gene="bglA"
                     /locus_tag="ACHOS5_000070"
     CDS             84815..86248
                     /gene="bglA"
                     /locus_tag="ACHOS5_000070"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP9774968.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYACNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFDWYKEVIASNGENL"
     gene            complement(86414..89287)
                     /gene="gcvP"
                     /locus_tag="ACHOS5_000071"
     CDS             complement(86414..89287)
                     /gene="gcvP"
                     /locus_tag="ACHOS5_000071"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP9774969.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(89405..89794)
                     /gene="gcvH"
                     /locus_tag="ACHOS5_000072"
     CDS             complement(89405..89794)
                     /gene="gcvH"
                     /locus_tag="ACHOS5_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP9774970.1"
                     /translation="MSNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(89818..90912)
                     /gene="gcvT"
                     /locus_tag="ACHOS5_000073"
     CDS             complement(89818..90912)
                     /gene="gcvT"
                     /locus_tag="ACHOS5_000073"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP9774971.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT        22572 a        24145 c        22399 g        22023 t
ORIGIN      
        1 atttgcgaca actcaataat ccgtactgat tcataatcaa ctgaattttc ataattattt
       61 aaaatatgca atgctataaa accaatctca tcatcaggaa gttgaacgct gaattgctca
      121 ttaatgatag ttaatgcatc gcgacttatc gcatactctg ctttgtacaa cgctttgatt
      181 tcatcaagga aaacattatt aatttgtata cctgaaacga ctcgggatat tgcattcgaa
      241 atgtgatccg caagaatcaa tagtagcgat ggattgagat ttttttcgag atttttctcc
      301 gcatattgca ctattttttc ggcaacaaac acatactcaa tatctacatg ttcaatcaat
      361 ttatagagtt tgtttttttg ttcatttctg acataaaaaa tccggttagc cggatgctca
      421 gggaccgaca ttccatattt tttgttgtaa ccgacgccag gcccagaaat gataacctcc
      481 tgtccattca tcgatgcctg cacacaatta ttgttcatga ctttttcgat gatcattcca
      541 tggctccaaa aaaaaaggca aggcacgtca gaagtaataa ctacttcaaa cattgccttg
      601 cctgatttaa cagttacaag atgattaact aatgcgtata cattacacgg aattgtgctt
      661 aattaaagag atggtcggct tatttttcaa gccgacattc aaatatccga catcagtcac
      721 aaaaccagcg tctaatattc aggtaaatgt tatctattca acgaaatata aattcattga
      781 ataaataaaa acattatccc cttctcgcca gcgtcgcccc atcggcaaaa tacgctttaa
      841 ttcccgcaag aatagactcc gcaacttcct gctggaaagt cgctgttttc agtttacgct
      901 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
      961 ttagtacggc aaacccagcc tgttcaactt gatttttatg cagcttgttg attttaccga
     1021 gcttattcag caccgcttta ccaaacttaa ggctgtcggc aatggtcagc gactgcacca
     1081 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gcttacgcca ccaatcaagt
     1141 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgcg cctttggttg
     1201 agagcgcaaa caccgaggaa ccgctcggct ggcgactggt aaaggcgtcg gcatggatag
     1261 aaacaaacaa gtcagcgcgc tgtttctggg cttttgctac gcgcacttgc aacggaatga
     1321 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc
     1381 gcagacggcg agctatttgc aataccacgt ctttttcgcg cgttttgtat ttccccaccg
     1441 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcccgcc
     1501 cagctttacc cggttgtgga ccactttgtg ctggcggcac ctgcttttcg aggtcgcctt
     1561 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
     1621 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
     1681 gttttacgtt ttgctttaat tcaaaaacca tacgtacggt ttgcgggtca aattgcccga
     1741 cgcgcgccga cttgatgaac gggtcgtcag cgcggatttg cgcagccatc cccttgagca
     1801 ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcaggatta ctcaacgcga
     1861 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
     1921 gccagacgcg caccgccacg acctggctga ccgcagccag gctgacctga cttacgctca
     1981 atagccacat ggcacccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
     2041 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgaccaattt
     2101 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta taaaacggta aacattcttt
     2161 ttatattcac ggtattactg ataaaaaact cgctcccgca taaaatttac acttgcaccc
     2221 tgcaaaaaaa cagaataaaa atacaataat ttcgaataat catgcaaaga ggtgtaccgt
     2281 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
     2341 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
     2401 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
     2461 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcacatcacc acgaaccgct
     2521 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggcagc
     2581 gggaacattg caactggata ttactgctcg cctgtcgatg agtctcaata acacaccgct
     2641 gcagggcgcg catatcaacg tcgtcagtgg taattttatt attgcccagc cgctgggcgt
     2701 cgatgacggc gtggattact gtcatagcgg gcgtatccgg cggattgatg aagacgcgat
     2761 ccatcgtcaa ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcggtcac
     2821 tggcgagagc tttaatctga cctcggaaga gattgccact caactggcca tcaaactgaa
     2881 agctgaaaaa atgattggtt tttgctcttc ccagggcgtc actaatgacg acggtgatat
     2941 tgtctccgaa cttttcccta acgaagcgca agcgcgggta gaagcccagg aagagaaagg
     3001 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
     3061 cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt
     3121 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
     3181 aacaatcaac gatatcggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat
     3241 tctggtgcgc cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca
     3301 gcgcgataat acgactattg cctgcgcagc gctctatccg ttcccggaag agaagattgg
     3361 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct
     3421 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
     3481 cacgcgcagt attcactggt tccaggaacg tggatttacc ccagtggata ttgatttact
     3541 gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
     3601 tttggggtaa gcgatgaaaa tcgtcggatg cgacatgcgt aacactcgtg cgtcgcatcc
     3661 ggcaattacg tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta
     3721 cgagtggcga ttgccgcact taatatgcgc tcatcggcat acagcgacag acggcgacgc
     3781 gcgcgggtca ccgcggtgta aaccagctct cgcgttacta ccggcgtgcg ttgactcggt
     3841 aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac
     3901 gtcgtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc
     3961 gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac
     4021 aacccaagcg cgctgtcatt acgggcaatc atcaccggtc ggccttcgta ccaacgagag
     4081 tgcggattac gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt
     4141 ccagccacgc caaacggccc ttcccgcagg gcgcacaaaa gctggtactc attgaacgcc
     4201 tgaatgatta aattcggctc ggcacgcgct tgcagcagat ccagataacg tccgtaaccc
     4261 gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag tcgtttttcg
     4321 atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttaatc
     4381 gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg
     4441 cagaggctgt cgcgcaaaga tgccgcgtct gtgccagttc ctgccggaac gtgagttccc
     4501 gtcaggcggc ttagctgccc ggcacgctcg ggggtaaagc ccgcgttggc ataagcgcag
     4561 atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag
     4621 atcactcgcg catgatcggg caaggcgtct atcagtctcg acatcatagg aagatcgatc
     4681 attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt
     4741 aaacgctggc tacccggctg cgcacccagc aatcggtgca aagtgctggc atcttccgga
     4801 atgcgtttct tttgttcatc ggtcagcggt aattgtcgca aagccttgcc gagagattcg
     4861 gttaatcgcg cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg
     4921 tcggccattt gaattaacgc tgccagtaac ttcgctacgg tggtcgtttt accggtgcca
     4981 ggaccgccgg aaatcaccga gatccgccgt gtcagcgcca ctgccgccgc aactttttgc
     5041 cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca
     5101 tcaacctcaa tggcatgatt cacttcgttg aaaaagcgtg ccactgtgcg ctcgttacac
     5161 cacatgcgat tcaaataaag acgatcgcca cagaggatca tcggcgttgg ttcatctccc
     5221 cggctgaccg cttgtgaagc cagcaagcat tcttcccaat tttgtagctc accgatttca
     5281 ctgacacagg tcgccaacag cggatgcgat gcctcgttat tttccagtcg tgaaagcggc
     5341 aaacaaacgt gtccctctcc ggcatcatga cttaatagtg ccgccgcgag ggtgacggca
     5401 ggatgttcat ctcccgccac ggtcagggca aactgcacgt ccagtgggcg tagctgtttg
     5461 tgctccacag cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac
     5521 cggcaaacat ctcatccatc aggtcaatca acccggcgtt gggtcgggtc gcgtagatcc
     5581 cttgttgcgg atgttcttta tcaacgccac gcaggaacag ataaataacg ccgccaaagt
     5641 ggcgctcata gtcgtaatca gcaatacgat ggcgcagata acggtgcagc gccagggtat
     5701 aaagctgata ttgcagatca tagcggtgtg cctgcattgc cgctgccata gcctgttggg
     5761 tgtaagccga actgtcttca cccaaccagt tggatttata gtcgagcagg taataacgcc
     5821 cttcgtggcg gaacaccaga tcgataaagc cttttaacat gccacgtacc tgcatgaact
     5881 ccagcggcgg gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc
     5941 tggcgataag cggttcacta atcggcaggt aaaactccat ctccacctgt ttatcgcgat
     6001 cggaaagctg attcaggcta acacccgttt cattgagagg tgccaggagg acagttgtga
     6061 tccactcagt caacaccggt tcccactgcg gttcaaagcc accgagctcc agtttttcct
     6121 gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga
     6181 atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga
     6241 cgctgaccac gcccgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt
     6301 gaccacgctg ctgcaaaccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt
     6361 gtagcgtcct cgcattcagt tctgcagtta atgcatcatt aacctgccag ggctggttat
     6421 caccagtttg tgccgtttgc caggcaatat catcatcgca caacgcttca atgcaggtac
     6481 gaagccctgc cgcatcttgc ggttctcctt tttgcagcaa acgcccgagc gcactttggt
     6541 ggacgtcggt atcacctttt ttatcgccac gacggcgcac cagcggtgca acaccgagac
     6601 tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagtaa acgcagatct tccgccagac
     6661 gttcggcctc cgcgaggtcg acgctttctg gcgcagcatt aagatccaga acggcctcaa
     6721 acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca
     6781 accagaccag cggatattcc agcccttttg atttgtgtat cgtgacaatc tgcaccagat
     6841 gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat
     6901 gttgcgataa ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgta
     6961 gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca
     7021 agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt
     7081 tgcgccagat ttgccgataa ccatcgaact cttcgacgac cgcatcccac gcatgttcgt
     7141 cattattcag cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac
     7201 tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacagccaa agcatctcct
     7261 gtgcttccag agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca
     7321 gcagcgttaa ggcatcgcgc acctgagcgg cctcctggcg gctgcgcacc agcacactga
     7381 tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccgcgtt
     7441 gcccggcttg taaccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat
     7501 agtcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt
     7561 cacctttaaa cacaaaacgt aacgcctgat ttttgccggc agatttcaca ggaataaacg
     7621 gtatttcacg aaacatgaac gcgtcatcag tctggctgaa aagtttgtta acgctgttca
     7681 ccattcccgg tgcggaacgc cagttggtat ctaaggtgta gtgagcatga acttcgctac
     7741 gcgccttcat ataagtgaag atatccgcac cccggaatgc ataaatagcc tgtttcgggt
     7801 cgccaatcag caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcgat
     7861 actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga
     7921 tcgccgctgc caatacctcg ccgctttcac tacgcagcgc ggaatcgagc cgacttaaca
     7981 tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct
     8041 cagccaatgc gcgagtgatc accagatcgc ggatcgacaa tggttctgca agcagttgtt
     8101 cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggctttcgtg cgatcttcta
     8161 agaaacgctg agagaatttt tccagcgact ccggcaactg atagctgttg gtctcttctt
     8221 ctgcccaggc gctgatcttc tcgatccatt tagcctgatt gctacggtta aacttgcgtc
     8281 gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct
     8341 gttttacggc atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat
     8401 cggggggcgg tgctttgata accggcgctt cgccttgcag ataacgatta atatcgcgca
     8461 gcaacgcctg cggccctttc caggtttcaa agacgacctg ggcaatttca cgcggcagcg
     8521 ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt
     8581 caatcagctg ctgctcaaac agcatgccgg attcaaaagc attcaggttg agcatgcgct
     8641 ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgctcg gctaacaaca
     8701 accactgcgc ggcttgcgct ttatcgtcga tctcttccag caggcgtttg tacagtggat
     8761 tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac
     8821 cacgcaattc tgccgtggca gcctcggtaa aggtcaccac cagcagttct tcaacggtca
     8881 gtgggcgggg aaaggcggcc gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg
     8941 caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg
     9001 gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc
     9061 attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat
     9121 tcggctttcc cattctggct gccggaaatc tgcgacagaa tagctatgcc ttgcggctcg
     9181 accaccgcct gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc
     9241 acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc
     9301 gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gagtaattac cgcctgctgg
     9361 atttgcgcaa actcctctgg cttcatcgcc cgcaattttg cctctgcggt tgggaaaaac
     9421 gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc
     9481 atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc caactgttct
     9541 tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag
     9601 gctgagctgg tgtattcatc gtagccagtc ggtacaaata tcgctgccag tgcggagtcg
     9661 gtgctgttac cagctttttc aaagatgacg gattgttttt tatcgaccac gacatctttg
     9721 ttacgacacc actctgaacc atcagcgccc aactgttttt gcacatggcg tgccagcgtt
     9781 gttgcctggg cttcagtcat gttgccgata accataaact ctggtctagc ccctgatttt
     9841 aaggcgtcgc gataggccag cacctctttc aacgtaatgg agggcaaaat tttgcgccgt
     9901 tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca
     9961 aacgctttgc ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca
    10021 agctgatctt ccgtagcggt atagctaaag tagccctcca gcagagcctg gaacagctgc
    10081 ggcaggcgct gagtgtaacc attggcatta accataaggc cgttgttagc attggtggaa
    10141 aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga
    10201 taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc
    10261 aaaatcaggc tgacatcagc tttgggctca ctggcaaaat aacggcttgg cgcatacacc
    10321 acgcgcagat tcgactcatc aacaatcaac tctggatggt cgtatttctt ctctgactta
    10381 atcagcgaga aatcgtcagg aatatagggg ttaagctccg gtaaggagag cgcaatattg
    10441 gcggcttttt tctgccagtc cgcgaaagtt tgttcgctga ttttatcgac ttgatacggc
    10501 gcatcgacaa aataagccgt tttattgtgc ggctctttcg ggctgatata ccagatacgc
    10561 gcattctgcg gcgtcatcat cgccagacgt tcttttactg ctttagcatc gtaccgatcg
    10621 gcaatattga ctgcatccag tgtatgctca acaggaacgc gaatcatggt atcagccagc
    10681 cattcgacgt aatccatatc acgggtgatt gacggataac ggaagtcgat atccagcaca
    10741 ttcgccagtt catcgaagta ttgtttatcg atcccttttt cacgtaacag attgaggtag
    10801 ctaaaaatgg ccgccacaac ctgatcgcga ttcgccagac ctttatcggt taaagacgca
    10861 gagatcgcta atacgccgct gttgccgttg acgataggat cggaattggc gctaataccc
    10921 tcaactaatc cctgcttttg cagccagtca gaaagtgtac ctgggctgcg attaccaatc
    10981 agataggtaa tcaattcatc cgttttacta cggaacttcg ctgaattgtt atcgatgcga
    11041 aactcaacgc gcagaacttt acgcggcaac gccgggacgt aatgaatgat aatgcccttt
    11101 tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg
    11161 cgaccaaagg tgtccgccgc cattttcgcc aactccggca acggtttatt gctgtaaata
    11221 accgccttca tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgt
    11281 accggattac ctggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg
    11341 gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta
    11401 cgcgccatgg ttaattcggc attcactgca ttacgctcac gttcggcgta tttcttgtcg
    11461 agcaagggtt ctgcaatagc atcggccagg cggtctaccg caccgggtaa ggcatcgttc
    11521 tcaacttcca gatagaaagc cgtgcgatac ggtgccgtgc tggcattgtg actgccgccg
    11581 tgcattttga gatattcggc cagactgtca gcctgcggat actttttcga ccccatcaga
    11641 ctcatatgtt caaggtaatg tgccagcccc tggtacgcct caggatcttc cagcgaccca
    11701 acgggcacca ccagcgccga gagcgattta actgcctgcg gatcagaaac cagtaagacc
    11761 accataccgt tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg
    11821 atggtttcct gaatgggctg ccatcccgtt tctgcctgac ttaagggtgc ccaaagggca
    11881 acaaacaaca ataatgcttt gaaccagatg ctgcggggca ttcacggacc tcataatcaa
    11941 ctttattttc tggccagatt caacacgtta acgcaaatca tctgccagaa tttaatcttg
    12001 tgctgcacga gtcagcctat gtctatataa ccatcagtcc gtgactggtg cgcatcataa
    12061 agtaagcgga tagattgcgc aatttttata caatactcat gactgattaa agcgaaacag
    12121 cggtaacagg aaacgttgcg actgttcaac gatagcctcc attgtctctg gtgttaattg
    12181 ccgccagagc cgttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc
    12241 gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc
    12301 atcgttttgc gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag
    12361 caatggcgcg gacattcctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc
    12421 ctgttcggct gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact
    12481 ttcaccatta ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg
    12541 cgccacactt aataaggagg gacgccagcg caacaggcca tccggctgca cctgcggcaa
    12601 ccagcccgtt atttgcacac cgttgcaggc gagatcaatc tccatactct gccccggctg
    12661 gcgacaggca atgactctgt cggcaagttg ctgcatctcc tgacactgcg tttcccagaa
    12721 aatttcacca aaggcaccat acggcaaatc acctgccgcc cggaatcggc ggaacaagcg
    12781 ttcggcatca tcctgctcaa ccagtacatt taataactgc tggttgattt gataacgact
    12841 aagcccttca agaataaaag gttcagtgtc gggaatttcg ctgtcttcgg tacggaagtt
    12901 cacctgcaaa cgcatctgga aaaacgcccg caccggatgt gcccagaatc gttgtagcgt
    12961 ttccagcggc acggtttccg gtaaggtaaa tggcagcggc tgaacaaatt cagaatgtgc
    13021 tttaccagcc tggctggccg caggtagcca ctcgcgcgca tagctttgta agttaccggg
    13081 ctggtagttt tgcgggtcaa acggcatccg ggtatggtgg caagtaagat gcgcttttac
    13141 ccttgcctcg ctttcatcac agttaagcgc ttcatcgccc ggtagataat ggctttgccc
    13201 gatgtagtcg atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat
    13261 ggaacgcccg atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa
    13321 cagatagcgg tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag
    13381 atcaaagccc aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca
    13441 aaccactttg aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc
    13501 gagaaaacgc tggctgatac gttcttgatc caggcgcagt gccagttcat cacgcaatag
    13561 tgacagcggc accgcgtcgc catactgcgc gcctaaacct tcggagataa tcgcctgcca
    13621 ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca tccggcagga aaaaggcgtt
    13681 gagcatatcg cgacaaaccg gcaaccactc ttccagcgga cgctcctgcg ccagcccgcg
    13741 acgccagata tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa
    13801 gccgctcgat tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc
    13861 gtagcccagc aacatgcgcg tcaggccaaa ccgccaggtg tgttgaccgg tagcgggaag
    13921 ttccagctcg cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca
    13981 ctgacgtaaa taacgcagcc cttcttcggt gatgtcaaac cgcgctgcca gcaccggcac
    14041 atccagtaat gccagcacgt cttccgacac aaagcggctg tctggcagcg ataacaggct
    14101 gataaacgct tcaagtacag gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg
    14161 caggtaacga tccgcaggtg cgctaccaaa cacagcctga ataaacggac tgtagctgtc
    14221 gatatcagcc accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cctccagcat
    14281 cgccagcagg cgatcgtgta aaacttcaac ttcacgctgc gggctatggc aaacgtggaa
    14341 ggtgatactg ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc
    14401 gatgttcaca ccagcaacgg cgcggttttc cagttccaga atgtcagact gaatattatg
    14461 cagcaggtta tctggcgtca catcgacaaa agcgtccagc tcctggctgc tctccaggtc
    14521 agaaaggaga taaatgtagt cgcgcccaag cttgccccat gaagccagca gcgggttgcc
    14581 gacatcctgt tcaccatcgc tgttaaagag ctgcccggca ttttcgctgt cgcgaaatag
    14641 cggtaattcg cgatcttcaa aactgtgtcg gcgctggcga gtcagtagtt tcgccagata
    14701 agcaggatct ttaatgtcgc cccagtaata acggcagggg ttggtaaaca ggagatggat
    14761 ttcaatatgt ttacccagcg cctgtagcgc ctggagataa acaggcggta acgcggaaat
    14821 accgcatata aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt
    14881 ttcgataaag cgctgataga gattggcgcg gtgccagcgc ggttgcccaa gttcgtcggt
    14941 atattccacc aacgccttcc acaacggcgc ttgccaggcc tgtgcttctc ccaacccttc
    15001 taccagatgt cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc
    15061 aaacaggtcc gccgcttttg aagaaagctg gaacagtttt cgcttgtcac tatcgtcagt
    15121 cagataatgc cgcaacaggg taaagtcttc gcgctccaac agttgcggca gcagagtcat
    15181 cagtttccag ctcatgctct gtttgttaaa ggcgctctct ttggggatct ccggtaacac
    15241 ccggacgaac atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat
    15301 accaaacttt tgcgacaggg tcatttgcag ccactgtgcc ataccggtac tttgcaccag
    15361 aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa
    15421 cgcttccagc acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta
    15481 ctgacgattc gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag
    15541 cgtaacgctg atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt
    15601 agcaggtggc gaaatcgcgt gcagttgcgt ttgctgccag ccatgccgcc agagctgttg
    15661 gtactggttt ctgctggcaa gactgttcat taatgttcgc tggaaacccg ataacgcagt
    15721 gacaatcatc accatcaaca ccatcgccaa cattacctcc ggcagactaa agccttgttg
    15781 attcctcagg gaagctgaca taacgccctc tctttcagcg gacaaaaatc gctccagccg
    15841 cgtggcgaaa aaaaaatgtt tccatcgatg acttcgcctg ttcgccacaa cgaaacgcct
    15901 tcataaccag caatcaataa ggcttcatta tctgccagta aacgcagaca aacccgggca
    15961 ccggtggcag catacagtaa gcactgaact gcgggcagcg tctgccagga gtgcattttt
    16021 ccccactcca gcgccgactg aacgatggcc tggcggcgca atgactgact ttccatgctc
    16081 acgcgagaag caaaactgcg atcctgctga ctcattcctt gcaatagcaa gctacccaaa
    16141 atcagcagca tcaggaccag agccagtgac gaaacaccct tttcgcggtt cacaggttgg
    16201 atcctgtcac gctatagctg gcatccacca cggtttgcgg atcagccttg ctggcagcgt
    16261 gcatattaac cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag
    16321 tgtcgataat gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg
    16381 tcgcaccgcg tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcggact
    16441 cttttaccgg ttcgcgatcc cagataccat tactgttcgc atcccactgc acaatgacac
    16501 agtcaccttg tccgacaatt tccagccctt cgcccgtaca gctgccatgg caataacccg
    16561 ccctctggag atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagcgcca
    16621 gcttacgggt gttcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca
    16681 gcaacaatac gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct
    16741 cttttacagg catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga
    16801 aaccactagc caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt
    16861 attgcgcagg ccaaagaaag caagcgaagg tgtcaggtcg ctcatttcga cttcgggcca
    16921 gcgcggcaca aagaccaatg gtgaactgcc atgacaggta ttcgccccag caacggaact
    16981 cacaaggcac cataacgtcc cctccctgat aagactgata ctgtggtcgc ggttatgcca
    17041 gttggcatct tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccaaag
    17101 ccgttgcgac tgctgccagt attgccagcc ataaagccca cttgcgctta gcatgaccag
    17161 aatcagcatc gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc
    17221 agtatggagc gaggagaaaa aaagacgagg gccagtttct atttcttcgg cacatcttcc
    17281 ggactattta cgccgttgca ggacgttgca aaatttcggg aaggtgtctc gaagaattta
    17341 acggagggca aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt
    17401 agatagccac cggcgcttta atgcccggat gtggatcgta tccttcaatc tcaaagtctt
    17461 cgaaacggta gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac
    17521 gcggttcgcg gcttaattgc agatgagttt gatccatatg gttgctgtag agatgcgtgt
    17581 cgccaccggt ccagacaaaa tcacccactt ccagatcgca ctgctgcgcc atcatatgca
    17641 ccagtaacgc gtagctggca atgttgaacg gcaggccgag gaagacgtca caggagcgct
    17701 gataaagctg gcaagagagt ttgccgtctg ccacatagaa ctggaagaat gcatggcacg
    17761 gtgccagcgc cattttatcc agttcgccta cgttccacgc tgaaacaata atgcgacggg
    17821 aatccgggtc gtttttcagc tggttcagta cagtagtgat ctggtcaata tgacgaccat
    17881 ccggcgttgg ccaggcgcgc cactgtttac catacactgg cccgaggtcg ccgttttcat
    17941 cggcccattc gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc
    18001 cctgcaggaa ccacagcagt tcgtggatga tggaacgcag gtggcaacgt ttagttgtca
    18061 ccagcgggaa tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc
    18121 cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta
    18181 aatactgttt catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga
    18241 ccatcatgat cacacccgcg acaatcatcg ggatggaaag aatttgcccc atgctgatgt
    18301 actgcaccca ggcaccagta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc
    18361 gaaacgcgcc gtaaccaatc aggaacaagc ctgagacagc tcccattggg cgcggtttac
    18421 gaatatacag gttgaggata ataaacagca ccacaccttc cagcagcagc tcgtaaagct
    18481 gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg
    18541 tttgcagcag caaaatatct tctgtacggg agccagggaa cagcatggca aacgggaagt
    18601 tcgggtcaac gcggccccac aattcaccgt taataaagtt gcccaggcgc ccagcaccaa
    18661 gaccaaacgg aatgagtggg gcaataaaat cagagacctg gaagaaggaa cgtttagtac
    18721 ggcgggcgaa gataatcatc accacgataa cgccaatcag gcctccgtgg aaggacatgc
    18781 cgccgtccca gacacggaaa agatacagcg gatcggccat aaactgcggg aaattgtaga
    18841 acagaacata accaatacgt ccaccgagga agacgccgag gaagcccgca tagagtaagt
    18901 tttcaatttc atttttggtc cagccactgc ccggacgatt cgcccgtcgt gttgccagcc
    18961 acattgcaaa aatgaaaccc accagataca tcaggccgta ccagtgaagc gccacgggtc
    19021 ctattgagaa aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc
    19081 accacaagtt cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt
    19141 tcaggcgctc caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg
    19201 ttaaaagata tgtatatgat ccgcgctata accccccgcg aatcagcccg cccatgccgc
    19261 gacgctccat aaaggcagca acctgatggc gaacttcggt cgccagttgc gcttccagac
    19321 tacgctgcgc aagattttct gcttcagcaa aatcaatgcg ccgcagcagg tattttacgc
    19381 gcgctacaga acgtccgttc atagacagat ggcgataccc aagcccaatg aggattgcca
    19441 cgcacatggg atcgcccgcc atttcaccgc acaaacggag atcgattcca tgtatttccg
    19501 cttcccgggc gatcatcgcc agagctcgta acattgcagg atgaagactg tcataaatgt
    19561 tcgccacccg ggtattgttg cgatcaacgg ccaggatgta ttgagtcaga tcgttggtgc
    19621 caacagagat gaaatcgacc cgctttgcca gatgcggcag cataaatacc attgacggca
    19681 cttccagcat gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt
    19741 cacgtccggc acgttcaatc aggcggcgtg cttcgtcaac ttcatcgagg cttgtgacca
    19801 tcggcaacag aatattcagg ttgcccgtag cggcattagc acgcagcatc gcccgcacct
    19861 ggatcaagaa gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg
    19921 gattctcttc gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac
    19981 gcaaggtgac gggtttatca ttaaacattt gcagcatccc ctgatactgc gccacctgtt
    20041 cttcttccga cggaaaacca ctttgcagca tgaatgggat ttcagtgcga taaagtccta
    20101 tgccatcaat acggctgccc agtttttctt catgttccgg gcttaaaccg gcattgagca
    20161 tgactttaat gcgttcgccg ctttttaact gggcgggtaa attgacgtca tcttccgcca
    20221 gacggctaag ctcgatctct tcactaatta gccgctgata ttcttgcagc agtaccggtt
    20281 ccggatcgac cagcaattca ccgcgataac catcgacgat cagcgtccga cgatgcagta
    20341 ccgaaggctg aatatccgcg cccatcacgg taggtatccc cagcgcacgt accatgatcg
    20401 cagcatggga gttggcagcg ccatcgcgca cgacaacacc gactaagcga tcctggggca
    20461 gctcagcaag cgttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg
    20521 cgttcggccc ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta
    20581 aatcgccagc ccgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt
    20641 tttcaatgac cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca
    20701 attcgcgacg cagccgggta tccgaaagca ggtgtgagta aagatcgaaa atagccgccg
    20761 tttctttttg tgcaccggca gcaaagcgtt tgctatagcg gcgaaactcg ttagccgcct
    20821 cttccaacgc cccggtcagt cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat
    20881 acacctgttc cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag
    20941 gtgctgccgg taatgcacgg attcgcgtct ggcgatattg cccaaacaag gcagtcaact
    21001 gcgactgaga aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac
    21061 tttcgtcata ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac
    21121 gttgaataat tggtacgcct aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt
    21181 atttgaagct ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa
    21241 ccaggccgac gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca
    21301 gtccccgggt cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac
    21361 agacctcggt atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca
    21421 ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataattggcg
    21481 tgacttaacc tcttttacgt cgataagcag atgcgttttg tggttttggc gtattttcct
    21541 gcagtgacat caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa
    21601 atgacaccac ctgtctgact ggataccagt aacttaccca tcgccagccg tcaaactctg
    21661 gtgtactgct ggtttgcata ttgatttctg catcgccgct caccagctgc aagagaaacc
    21721 atttttgttt ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta
    21781 atttgtagcg caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc
    21841 ctacttcttc aaacaattca cggtacatcg cctgctctgc ggattctccg gggttgattc
    21901 cgccttgcgg aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct
    21961 ggcgattaca aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac
    22021 tacctcaaaa taaagcttta tatacgaatg attgtttcat actccaggaa gacggtaaac
    22081 cactctctgc agggcattac acactaataa caattgaata actcacagtt atgtgcagag
    22141 ttataaacag aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg
    22201 tgaagaacta cggaattact acgggaaaac ccggagcatt ctgaataaga cagatacaga
    22261 gattttaaaa tattcattat cagtcaatgc attacatgtg atttgagtaa taaaaaagag
    22321 taattcgtga cccaggtcac acctctcatt tacgggttgg cgaaagatca accaatgccg
    22381 tatttatcca cagaatgtgc cactaagtta agcactgaac cactaaaaac tggagtttcg
    22441 tcgcacgtca aggctgtaaa tggaaacagt agtgaaggtt tttcacagtt atcccagctt
    22501 tctgtggata acatggtgta agatcctgtt tattttcagt gaccagattt ggaaaacccg
    22561 ttgcagtgtt gcgcaactcg attaccggca acctaaaaag caatataaat cagagaattg
    22621 aacaacgcat gtggaaaaag ttacactgcg aatattcggc acttaattgc tgtttgtttt
    22681 ttaaccaagg tatcatgaca tgtcccaacc tcgcccactg ctctctcctc ccgaaactga
    22741 agaacaattg ttagcgcaag cacaacaact ttctggttat acattgggag aactggcggc
    22801 acttgccggg ctggttacgc cggagaattt aaaacgcgat aagggctgga ttggcgtgtt
    22861 actggagatc tggctaggtg ccagcgcagg gagtaaacct gagcaagatt ttgctgctct
    22921 gggcgtggaa cttaaaacta tccctgtgga tagtcttggt cgtccactgg aaacaacatt
    22981 cgtttgtgtt gccccgttaa cgggcaatag cggggtgacc tgggaaacca gccacgtgcg
    23041 ccacaagctc aaacgcgtgc tgtggatacc ggttgaaggc gagcgcagca tcccgctggc
    23101 aaagcgtcgc gtaggatcac cgttgctgtg gagcccgaat gaagaggaag accggcagct
    23161 acgcgaagac tgggaagaat taatggatat gattgttctc ggtcagattg agcggatcac
    23221 cgctcgtcac ggggaatatt tacagatacg accgaaagca gcgaatgcga aagcgctgac
    23281 cgaagctatt ggtgcccggg gcgaacggat tctgacgctg ccgcgcggat tttatttgaa
    23341 gaagaatttc accagtgcgc tactggcccg tcattttctg atccagtagc catcgctttg
    23401 acctgccgct ttccgggcat ataattaccg cttcattttt ttggcagggc tttttagatg
    23461 ttatttgcat ggataaccga tcctaacgcc tggcttgcgc tcggtacact gacgctgctg
    23521 gagatcgttc ttgggatcga caatattatt ttcctttctc tggtggtggc aaagcttccc
    23581 acagcacaac gtgctcatgc gcgccgtctg gggttggcgg gagccatggt catgcgtctg
    23641 gcgctgctgg catccatcgc ctgggttacg cgcctgacga atccgctttt tacaatattc
    23701 agtcaggaaa tttccgcccg tgatttgatc ctgcttctgg gtggcttgtt ccttatctgg
    23761 aaagccagca aggaaatcca cgaatccatc gaaggtgaag aagaagggct gaaaacacgc
    23821 gtttcatcat tcctcggcgc tatcgtgcag attatgctgc tggatattat cttcagcctc
    23881 gactcggtga ttaccgctgt gggtctgtca gatcacctgt ttattatgat ggctgccgtg
    23941 gtgattgccg taggcgtgat gatgttcgcc gcgcgctcga ttggtgattt tgtcgagcgc
    24001 catccttcgg taaaaatgct ggcgctctct ttcctgattc tggtgggctt taccctgatt
    24061 ctggaaagtt tcgacatcca cgtaccgaaa ggttacatct acttcgcgat gttcttctct
    24121 attgcggttg aaagcctcaa cctgattcgc aacaaaaaga atccgctctg atactccgta
    24181 cgctctcctg cgggagggcg tactcactcc tgttaccctc acagtttaag attttcctgc
    24241 tttcaagata tataacgtcg gtttataaac agactattat cataggtggg ccaaatgagg
    24301 ataaccggat gaaaaaatgg gcagtaataa tttctgcagt cggactggcg tttgctgttt
    24361 ccgggtgttc cagtgattac gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg
    24421 gaaaacctga aattgatgat gataccgggc tggtgagtta tcacgatcag caaggtaacg
    24481 cgatgcaaat taaccgtgat gatgtttcgc aaattattga acgttaacaa ataaggtcag
    24541 catccggctg gccttaagat ttttctcttt cccttttccc ttccctctgc catttttata
    24601 ttccttatgt cgtgattata aaaaggaaac ggctatgcaa tatcaccgta taccccacag
    24661 ttcgctggaa gtcagcacgc tggggcttgg cacgatgacg tttggtgaac agaacagcga
    24721 agccgacgcc cacgcacaac tcgactatgc cgtcgctcag ggcattaacc ttatcgacgt
    24781 tgccgaaatg tacccagtac ctccgcgccc cgaaactcaa gggctaaccg aaacctacgt
    24841 cggcaactgg ctggcgaaac atggcagccg cgaaaagtta attatcgcct ccaaagtgag
    24901 cggaccgtcg cgcaataatg acaaaggcat ccgcccggat caggcgctgg atcggaagaa
    24961 tatccgcgaa gcgctgcatg acagcctcaa gcgtctgcag actgattacc tcgatcttta
    25021 tcaggtgcac tggccgcagc gcccaaccaa ctgcttcggc aaactcggtt atagctggac
    25081 agattctgcg cctgcggttt cgctgctgga tacactggac gcactggcag agtaccaacg
    25141 cgcgggaaaa attcgttata tcggcgtgtc gaacgaaact gcatttggcg taatgcgcta
    25201 cctgcatctg gcggacaaac acgatctgcc gcgtattgtc accattcaga acccctacag
    25261 tctgttaaac cgcagttttg aagtaggtct ggcagaagtc agccagtatg aaggggtcga
    25321 actgctggcc tattcgtgcc tgggtttcgg cacgctgacc gggaaatatc tcaacggtgc
    25381 aaaacccgct ggcgcacgta atacgctctt tagtcggttc acccgctata gcggtgagca
    25441 aacgcaaaaa gccgtcgcgg cgtatgttga tatcgccaga cgtcatggcc tggatcctgc
    25501 acagatggcg ctcgcttttg tacgccgtca accgtttgtt gccagcactc tgctgggcgc
    25561 aaccacgatg gagcagatga aaactaacgt cgaaagtttg catctggagt taagcgaaga
    25621 cgtgttggca gaaattgaag cggtgcatca ggtttacact tatccggcac cataagatta
    25681 acgccggtta aaaccggcgt taaatattaa tgacggcgct gccagatcca cagcgccgtt
    25741 attgccagcg caaacagcgc accgaagcca atgccaatgg gcacgaccgg gatgcctacc
    25801 attaccgcca gcgagtaaat gcccagcatc aacaacatgg cgctgttttc gccaaggttt
    25861 tgtactgcaa tcgcattccc cgccccgacg ctttttttac cccgctcctg tagcaacgca
    25921 ttgagcggaa cgacaaaaaa gccccccagc acgccaatca gcatcaacaa agcataggct
    25981 ggcagcagct cgtgttgcag ggagaaaatc agtaccacca cgccaatcaa aatcccggct
    26041 ggcatacagc gtgacacggt ttccagcgta actaacttcg ctgccgcacc tgcaccaacc
    26101 acgataccaa tcgctaccat cgcgttgaga taggtcggcg tagcgttatc ggtaataccc
    26161 agcgccaccg gtacccacag caccaacagg aaacgcagcg tgacacccgc tccccagaat
    26221 aaactggtgc ccaccagcga aaaacgcgtt tcaccattgc gccatagcga ggtgcaggca
    26281 ttcaggaaac tgcgggtcat gctgatgaga ttccaggact gccccggacg cgccgccgcc
    26341 agtttgggaa tgtagatatt ggcaacgacc gcgccaccgt aggccagtgc gcatgcgacc
    26401 agggcaacca gaatatgcca gtcagccaga acaccaccag ctacggaacc gagcaaaatc
    26461 gccgctatgg tagacgcttc cattaaaccg ttagctttta ctaacttact acccgtggtt
    26521 aattcgccga gaataccgta tttcgccggt gaataggctg cagcaccaac acccaccaac
    26581 gtatagccga gaaacggatt gataccaaag cagatactgg ctgcgcccag tagcttcagg
    26641 ccgttggcaa acatcatcac ccggcctttg gcgaagctat ccgccacctg cccgacaaac
    26701 ggcgcaaaaa gaatgtaagc acctacaaac accatttgca ggatgggctg gctccactcc
    26761 ggatagaact gcgctttcag taacgccaga gtggcaaaca gtagcgcatt atcgccaaac
    26821 gcagagagaa actgcgccac gataaccgct ttcatcccct tcgaccacaa cgaagtatta
    26881 gtgtgcactg actcactcat cgtgttgttc cgcttcgtct acccagcttt tcaaagtgac
    26941 aaagtcaggt ttgccgctgc caagtaatgg catctgtttc agatagcgaa tatcgcgcgg
    27001 tacagcaagc tccggcacgc cgtgctcgcg ggcatactgt tgtaacttat cgcgcgtcag
    27061 ttcgttatct gtggtgaaaa gcaccagtgc ctcgcctttg ctggcatcgc tcttaatcgc
    27121 agtggcatgg actttatctg gcgaaacacc aagtgccagt tgttccacca tttccagcga
    27181 caccatttcg cctgcaattt tggcaaagcg ttttgcgcgg ccctgaatct gcacaaagcc
    27241 ctgctcgtca aaacgcacaa tatcgccagt gtcataccag tcgcgctcca tttcgccgcg
    27301 aatattctcg gcggtgggca cttccagtac accaggcttc tccacccgca gatagccgtt
    27361 cattatgttc ggccctttca gttgcaggcg tccgccctct tcgataccag gaaccgacaa
    27421 caggcgcgca tccattcctg gtagaatacg ccctaccgta ccgggtttcg ccgccatcgg
    27481 tacgttgata gaaacgacag gcgcgcattc ggtcacaccg tagccttcaa ggatgcgcag
    27541 gccaaattta tcctgccaaa gctgtttagt actttcttgt agtttttctg cgcctgccac
    27601 cacatagcgt agacgataga agtcatacgg gttggcgaaa cgcgcgtagt gaccgaggaa
    27661 agtggaggta ccgaacaaca cggtgcaact gcggtcatac accaactccg gcacaatacg
    27721 gtaatgcagc gggcttggat aaaggaacac ttctgcacct gtgagcagtg gcgtaaacag
    27781 gcctaccgtc agcccaaagg agtgaaacag cggtaacgcc gacataaagc gatcgttggt
    27841 ggtgaagtcg gcaatcgttt taatctgctc gacattcgcc agaatgcttt tatggctatg
    27901 gacgacgcct ttcggatggc cttcagaacc ggaggtaaaa aggatcagcg cctcttcttc
    27961 cggctgctgt tttacctgtg ccagacgcgg catcagcaaa tgagcgaaaa tccacacttt
    28021 gtcggtagtg gtgacatctg cttttaaatc ttccagatag acccagcgca cctgagtaag
    28081 ttgctccggc agatgccaga gtttgccttt atcgagaaac tggcgggaag tgaagatggt
    28141 tttgatttca gccgccgtaa tagcactggt cagccctttt accccggcgg tgtagttcat
    28201 cattgcggga atgcggcgac gggcgatggc cccaaaaatc actgcagcac tgatgcctgc
    28261 attgggcagc attaagccga tgcgttcgcc ttcaacactg tatttttcaa ggatgcgtcc
    28321 aacaaacagc gttttcgtaa gcaatttgcg ataggagtcc ggggtaaagt taacgtcttc
    28381 gacacatttc ttcccggctc cgaagcggta cattgcgctc agtaaagatt cgtacagcgt
    28441 ttcacgcggg cgcaccgcca tacgcgcttc catcattatt tgatgcagca tttcgccagc
    28501 gattttgcga cggtcacggg cacgcggcgc atctggcatc tccacctgcg ttggtggcaa
    28561 aatatgcaga gtaatttgcg ggaacaagcg acgtttaacc agacctttca ggcggctgaa
    28621 gtgcgtaagt tccgcccctt caatacgcac aggaataacc gttgcaccag acttcgccgc
    28681 gacaaatccc gcgccatcgt agattttcat cagcgagcca gtggtggtga tgcgtccttc
    28741 agggaaaatc accactggtc gtccctgttc caccagacgt accagatgtt taatcgccat
    28801 aggttgcgtc gggtcgagag gaacaaagtc gataaatgat ttcagccaac gcatatacca
    28861 ctgttggctt attgaggtgt aaacggcaaa taccggacgt acgggtaaaa acaatgccag
    28921 cagtatgcca tcaataaaag agacgtgatt aggcgtaatt agaacgcgct cgcccttcag
    28981 tgccttggtg tcacccgtaa cgcgaacgcg atacaaaaca cggcacaaat ttcgaaaaaa
    29041 gctaaaaagc attccaactc cctttgctct gattcagtaa aagcgaatgg agggagatta
    29101 cacgagataa agaacgcgag cgacagtaaa ttaggtgcga aaaaaaacct gcgcatccgc
    29161 gcaggttggt gcaagagact gggtacgaag agcgtaccga ataatctcac caatcaatac
    29221 ctctgggatc ttgattgtgg tctgcacaac gactcttcgc cagcgagaaa acgcaaagga
    29281 atgaagggaa atgcaacgag gtgtgtaaat tgtcggttac tgttacagat tgatgaccgg
    29341 caaaaaaaaa cctgcgcatc tgcgcaggct ggtgtaattc atgtgctcaa cccgaagttg
    29401 acttcaccta tcaatacctc tgggatcacc actttagcaa cctgaagcca aacgccacca
    29461 gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc tctttttttg
    29521 tcttacttat ttgcgtttgg ctcacattcc cacaatgaaa acacgccgcc ccttgaacca
    29581 acgggcgttt tccgtaacac tgaaagaatg taagcgttta cccactaagg tattttcatg
    29641 gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt ttcccgcgtc
    29701 attaataatt cacccaaagc cagcgaagct tcccggcttg ctgtgcatag tgcaatggag
    29761 tctcttagct atcacccgaa cgccaacgcc cgtgctctgg cgcagcagac cactgaaacg
    29821 atcggtctgg tcgttggtga tgtttccgat ccgtttttcg gcgcaatggt gaaagcggtc
    29881 gaacaggtgg cttatcacac tggtaatttt ttattgattg gcaacggtta ccacaacgaa
    29941 caaaaagagc gtcaggccat tgagcaactg atccgccatc gctgtgctgc gctggttgtc
    30001 catgccaaaa tgatcccgga tgccgattta gcctcattaa tgaaacaaat gcccggtatg
    30061 gtgctgatca accgtatcct gcctggcttt gaaaaccgtt gtattgctct ggacgatcgt
    30121 tacggtgcct ggctggcaac gcgtcattta attcagcaag gtcatacccg cattggttat
    30181 ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta ttacgatgcc
    30241 cttgctgaaa gtggtattcc ggccaatgac cggctggtga catttggcga accagacgaa
    30301 agcggcggcg aacaggcaat gaccgagctt ttgggacgag gcagaaattt cactgcggta
    30361 gcctgttata acgattcaat ggcggcgggc gcgatgggcg tgctcaatga taatggtatt
    30421 gatgtaccgg gtgagatttc gttaattggc tttgatgatg tgctggtgtc acgctatgtg
    30481 cgtccgcgcc tgaccaccgt gcgttaccca atcgtgacga tggcgacgca ggctgccgaa
    30541 ctggctttgg cgctggcgga taatcgccct ctcccggaaa tcactaatgt ctttagtccg
    30601 acgctggtac gtcgccattc agtgtcaact ccgtcgctgg aggcaagtca tcatgcaacc
    30661 agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcaatgg tctggcgacg
    30721 gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg gacggctgtt
    30781 gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca gataatcacc
    30841 cgccttcact tccggcaagg cgcgggtttc gacatttccc ccttcctgct gggtaaagac
    30901 atcgcccgat tcacataacg gcccggcgac gacggtttcc accgttggcg cgtgttccag
    30961 agaacgaccc tcagctgcca gggcactgat atggtggtaa ctaccgtaca ttgccgggcg
    31021 catcagatcg ttgaacccgg catcaaccag cacaaagtgg cggctaccca tttgtttgac
    31081 gctccgcact tgagtaatta atacgccagc ctgcgctacc agaaagcgac ccggttcaat
    31141 ttccagtttc acaggatggc ccaaatggcg ggcgatttgc tcacgcgcgg cattccacag
    31201 accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa tagaaagccc
    31261 accgcccgca gaaatagcct gtaaatcctg accgaactcg aggacctgac gcaccatagc
    31321 accacatacc tgttccagat gggcataatc gacgccagaa ccaatgtgca tgtgaatgcc
    31381 gaccagctgt agatgatgac gttgtatcac gtccagtgcg gcgggcagat cggtgtacca
    31441 gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgcc cgtgaccaaa
    31501 ccccggatta acacgcagcc atacccgatg ccctggcgaa acctgaccca gttggtcgag
    31561 catatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt caagcgtcgc
    31621 ctgatcgata acatctgccg taaaaacaat atcatcgggg tacgtttgcg gattgtaacc
    31681 cgccgccagt gcacgctcta tttcgcctaa cgagacggaa tccactttta cgccctgctc
    31741 acgcattaag cgcaaaatat gaatattgga acaggctttc tgtgcaaagc gcaccacatc
    31801 aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga cccacaccgg
    31861 gcagccaaat tctgcgggca aacgcagcag attttcggcg gtgagatcgg tatcagtgct
    31921 gaacagtgaa tgtggcataa caaactccag ataagtgctt ttttatgatt acgccacatc
    31981 ataaaaagaa taaaaaatat cgatttatgt cgagtctatg caaaaatgat atggatcacc
    32041 ggaatgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa tttttcatgc
    32101 ggtaatgacc gccggaagcc tgactgaggc ggcacacctg ctacacacct cacagccaac
    32161 cgtcagccgc gaactggcgc gctttgagaa ggtgatcggg ctgaaattgt ttgagcgcat
    32221 acgtggacga ttacatccta ccgtgcaagg actgcgcctg tttgaagaag tgcaacgatc
    32281 ctggtacgga ctggatcgca ttgtcagtgc cgcagaaagt ctgcgcgagt ttcgccaggg
    32341 agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc aactcctgca
    32401 accctttctg gcacgttatc ccgatgtcag cttaaacatc gtgccccagg aatcaccgct
    32461 acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaaa cgctccatac
    32521 gcctgcggga acagaacgta ccgaattact ctctttagat gaagtgtgtg tgttacctcc
    32581 gggccatccg ctggcggtaa aaaaggtatt aacgccggat gattttcacg gtgagaacta
    32641 catcagcctt tcccgtactg acagctatcg ccagttgctg gatcaattgt ttactgagaa
    32701 tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcagtct gcgcaatggt
    32761 acgggcgggg gtaggcgttt cggtggttaa cccgctcacc gcactggatt atgcggcaag
    32821 cggtttagtg gtgcggcggt tcagcattgc ggttccgttc actgtcagcc tgatccgccc
    32881 cctgcaccgc ccgtcatcag cgctggtgca ggcgtttagt gagcatttac aagcgggatt
    32941 accgaaactg gtcacttctc ttgacgctat tttgtcgtca gctacgacag cataaaagcg
    33001 acagcatcct cggcatggat cgccgcggta tcaaacacag gcagaacact gcgctcttct
    33061 ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc ctgttctgca
    33121 aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattg ccccagacac
    33181 agttcttcaa aaataatctg attaattttc gctcgttcat ccgcttcagg aataagacag
    33241 ttgatggaaa attgttccgt cagccgcccg cgataaaaat cctgttccat ggtgtaacgt
    33301 gtacccagca gcgccacacg agtcattcca gcccggataa ttgcacgtcc agtggcatcc
    33361 gctatgtgta agaaaggcag agagcaacga gactcaatgg catccgccac tttgtgcatc
    33421 gtattggtac ataacacaat accttctgcg cccgcccgct gtaagccaag cgccgcctca
    33481 gccagaatat ccccggtttt gtcccactca ccgcgacgct ggcactcttc tatttcatga
    33541 aaatcgacgc tatgtagcag cacttgcgca gagtgaagcc caccaagccg ctgtttaatg
    33601 ccttcattta tcagacgata gtaaggaata gtggattccc agctcattcc tcccagcaaa
    33661 ccaattgttt tcattcctct ctcctgatgt gtgttaccgc aacaaggctg aataaggaac
    33721 gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag ggccggatgt
    33781 acagcacatc cggcccgtga aatcagacgc cgatatttct caacttttcg cctgccatca
    33841 gtttgcgctc gatatgttcc agcgtgacat ttttggtttc cggaatgagc cagaaagtga
    33901 tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa cgtaccggca gcgccaatgc
    33961 tatcaagcag tgtcaggaag gtcgcgccga taatcatatt cgacacccag tttgtcgtcg
    34021 ttgagcaggt gatgccaaaa tcgcggcatt tcagcggctg aatttcagag cacaagatcc
    34081 acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc atgccaacag
    34141 agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg cagtaaccca
    34201 gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggc ttacgccctg
    34261 ctttatctac cgtaaagacc gcaatgaagg tagcgaacat aaaggtcagc cccacgacca
    34321 gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag atacgtggcg
    34381 cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac aacataccga
    34441 gaaatacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg ccctgtttta
    34501 acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca
    34561 gcatacgcaa tacttcttcc gcctcaatat gacgcccctt ttccgccagc cagcgcgggc
    34621 tatttggcag gaagaccacc agaataatca gcagaactgc tggtaaagca agaaccccca
    34681 acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac gccagcacga
    34741 tgcctagagt gaccatcaac tggtacatgc tgatcatctt accgcgaacg ttttcacttg
    34801 ccatttcaga aagatataga ggagcggtgt aagacgcgat cccgacagca atgcccaaca
    34861 ccacacgagc ggcgattaac atctctacgc tggtcgcaaa agcggaccct atcgaaccga
    34921 gtacaaacag gatggccccc gccatcagac tgtatttacg ccccaggcgg aacgacagcc
    34981 aaccattaaa cagcgcacca attgccgcgc cgagcatcat gctactgacc acccattcct
    35041 gcaaacgact ggtcagcaca aagtgatcgg ttatgaacgg caacgctccg gcgattacgc
    35101 cgatatcaag accaaataac aatcctgcga ccgcagcagc taccgaaaca aacatattca
    35161 tacgccgcgt atcccgcaga ggacgtggcg ttaaagcaga ttccgtattg atagtaacca
    35221 ttttttcctg ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa ttcacacagg
    35281 tgtcaggtcg gaaatagcat aaatatggat taaattgcta cggcatgtcg ttatgtgatg
    35341 gatattccaa ttttcaaatt aagttgaatt attgagatta ttattaacca cctaatttta
    35401 aagcagataa aattcataaa gttcatcaat tgataattga tatggattat ttcataacca
    35461 tgatatggat tatgatgatc tacaggtata aaaaaccctg ccatgcgaca gggtcataaa
    35521 agtaagagga atgaattaac gcgccagcca accgccatcc acagcaatgg tataaccatt
    35581 cacataatct gaagcgctgg aggcaaggaa caccaccggc cccatcaggt cactcggcag
    35641 tccccaacgg ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg
    35701 cagttgttga gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt
    35761 gtgtttagcc cattcgttcg ccatcaatcg cgtcacgccc atcacaccgc tttttgatgc
    35821 ggtataagaa ggcacacgga tcccgccctg gaaggagagc attgacgcga tattgataat
    35881 cttgccgcca ttgccttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac
    35941 gctcttgata ttcaggttca tgacatcgtc ccagtctttt tcgctgaatt cgagagcgtc
    36001 ttcgcggcga atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac
    36061 cgcgcgatcc agcagtgctg gaatgccatc aatctttcgc agatcggcgg tcaggcttaa
    36121 aaaacgacgc cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat
    36181 gccaacaatg tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagcccagt
    36241 atcacaacca gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac
    36301 aataccttat tcgtttatgc ccacaactag cgcaaatctt taacggccac atggtccata
    36361 tcatcaaaaa cctggttttc accgaccatg ccccagataa aggtataagc tttggttccg
    36421 acgccggaat ggatcgacca gctcggggag atcaccgcct gctcgttatg catcacaatg
    36481 tgacgcgttt cttgcggttg ccccatcatg tggaaaacgc aggcgtcatc atccatattg
    36541 aaatagaaat aaacttccat ccggcgctcg tgggtgtgac acggcatggt gttccacaag
    36601 ttacctggag ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg
    36661 acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga
    36721 gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg
    36781 taataaaact tcgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct
    36841 ttaccaacat acaaggcgtc gcggtgaccg atttcatagc attgaccatc gacagtaatc
    36901 gtaccggcac cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg
    36961 ccgagttgtt taccaacttc cccgccaacg gaaaccgttt tagttaccgg cataatgccg
    37021 ccaacaataa tacggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc
    37081 ttttcaacca aaaattcatt gcgcagcccc tgggtatcca gcgtttttgc gtgcgcactg
    37141 tggatgctct gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa
    37201 aacaacaatg aaacactgtt tcgttttctc tatgagggga tgatatgccc atgggatagg
    37261 gttttcaatt aaaaataaaa caatgtttca ttttcaagtt aaagattaaa aaaagtgcag
    37321 tgtcgatcac gaatatgtca tgagaatgga aaaatcaggg agccctgaaa aggtatgtcg
    37381 actcaccagc catcattatt caacagatta atgacggcag atatttatcg gtaaggagga
    37441 aatgcagagc tactcgtcac gttcaatggt caatgccaca ccctggcccc cgccgataca
    37501 aagcgttgcc agtcctttgc gggcattacg tttcaccatt tcatgaacaa gagaaaccag
    37561 gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg atcgcaccac cattgacatt
    37621 gacccgacgc tcatcccatt caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc
    37681 ttcattagcc tcgataagat cgacatcagc caactgccag ccaacacgct ccaggcaacg
    37741 gcgggtcgca tacaccggcg caattcccat caatgccgga tccacaccaa cgctggcaaa
    37801 tgcgcggatg cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat
    37861 cattactgca gctgccccat cgtttatgga tgatgcatta cccgctgtca cagaaccgag
    37921 actatcaaat gaaggattta aacgggctaa gccttctgcg ctggtgtcag tgcgtggctg
    37981 ttcatcggta tcaacaacca acgtctgtcc gttactttgg gttattaccg ggacgatctc
    38041 atctttaaat cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta
    38101 agcatcctgc aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc
    38161 aatatgataa tcattgaagg catcccacaa cccatcatgc acaagactat caaccaactg
    38221 gctattgcca agctgtgcgc cggtgcggct atcagtcaga acatgtggtg cgcggctcat
    38281 gttttcctgg ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc
    38341 cagatgcagt gctttaagcc cggaaccaca aacgtcatta atagtgattg cagaaacgct
    38401 attaggaaga ccacctttaa tagccgattg tcttgccgga ttctgccctg cccctgcagt
    38461 caacacctga ccaagaatca cttcatccac cgcatatgca ggaacgccgg tacgttctat
    38521 taacgctttg acgaccaggc tgccaagttc cacggcggaa tgacccgcta acgcaccacg
    38581 aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc
    38641 gcaccaatta acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa
    38701 aaaggtgatt tttatcacaa aggaaatacg cctgagtagc agtcagagac ataactggca
    38761 cgtaaggttt gcaaccacta acccaccaat agagaggtag atagggcgtt aatctcccat
    38821 acttaacctg gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc
    38881 agataatgtc taatatttgg tcaaaagaag aaactctgtg gagtttcgcg ctctacggca
    38941 cagccgttgg tgcaggcacg ctcttccttc ctattcagtt aggttcggcg ggggctgtgg
    39001 tcctgtttat tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat
    39061 gccagttcat cctctcatcg aaaacatcag caggtgaagg gataacgggc gcggtaacac
    39121 actactatgg caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg
    39181 tcgtcgtgtt gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc
    39241 atatggttat tgatcttcgc atccgtatgc tagtgagtct gggtgttgta ttaatcctga
    39301 atctcatttt tctgatggga cgccatgcaa ccattcgggt aatgggattt ttggtattcc
    39361 cattgattgc ctatttctta tttctttcta tttacctggt cggtagttgg caacctgatc
    39421 tattaacaac ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc
    39481 ccgtgatggt tttcgctttt agccatacgc ccattatttc tacgtttgcc atagacagac
    39541 gtgaaaaata tggcgaacat gctatggata aatgcaaaaa aattatgaaa gtcgcttatc
    39601 tcatcatctg cataagtgta ttgttctttg tctttagctg cctgctttct attccacctt
    39661 cgtatattga agcggctaaa gaagaagggg ttaccatttt atcggcgctt tctatgctgc
    39721 cgaacgcccc agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga
    39781 aatcattcct gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc gtcaaaacaa
    39841 cattacagca ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt
    39901 tggtatcgct gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt
    39961 acgcgatcag cggcccgctc attgccatga tacttttcat catgcctacg ctgtcaacgt
    40021 atctcatccc ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg
    40081 gtattctgtg cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt
    40141 gcttcaattt tgaaccatcc cagttttcat aatgttcatt tgttattatt ccgctctatc
    40201 acttctctcg taatattata taattatcag atagttaaac acacaaagag ttctgaacat
    40261 cttaaataag actatttaag atacatgacg attgcattgc gtaaacgctt tttatttaca
    40321 aaaaaatggg gaagtattac ggcgattgcg aaaagtctga agccttgaat taagataata
    40381 tagaaaatca gatacaacaa aaatggcagg tagatgatgt gaagataaat tggagcgggc
    40441 gaagggaatc gaaccctcgt atagagcttg ggaagctctc gttctaccat tgaactacgc
    40501 ccgcttcgag atgcgtaagg cattataaac cttacgctct ccttagcaag taccacgctg
    40561 ctgactgctg attaattcgc catcagcatt ttggcttgct gccctgaggc ggcaagtaac
    40621 gtagcggatc aattgccgtt gcacggtaac gaatctggaa atgcaggcga acagatgccg
    40681 catcagtgct ccccatggta gcgatttttt gcccagcctt cacgctttgc ccattattta
    40741 ccagcatcgt gtcattatga gcgtaagccg taatgtaatc ttcactgtgt ttaatcatga
    40801 tgagattacc gtagccacgc agttggttgc ccacatacac cacctttcct gcacccgcgg
    40861 cgtaaatagg tgtaccccgt ggagctgaga tatcaatccc tttattgccg ccatctgctg
    40921 tcgaatacgg catgataact ttccctgtcg ttggccataa ccaacaacgt tgccctactg
    40981 gcggccagga agatttcggt accgctgatg acggtgtaac cgatgcggtt ttggtcgttg
    41041 atttggcggt tgatttacgt gtactactgc tacttttcgc cccacccagt tttagtttct
    41101 gacctacttc aatggtgtaa gggggggaaa tgccgttcag tcgcgccagt tcttttacgc
    41161 tggttcccgt ggtgcgcgaa atacgatata gcgtatcccc ccgtttcacg gtgtaaacgg
    41221 agccggaata cgttcctgta tcggatgatt tgctacccga acagcccgcc aaaagcagtc
    41281 caaccgataa caacatcacg atacccagag attttttatt caggcgtccc gcactcaaaa
    41341 tctttcctcg cttaaaaaac aagacgccat atcatagcag ccagcctctt gatacccaat
    41401 ctcttgcttc tggaaaaacg atgtaataga gaaagaggtt aatttcattt ggcaaaggga
    41461 agtatcatca gcaaaatatt tgctgtagtg gtatcgaagt tcctgacttt taccctattt
    41521 cccaccttga tgcgtaataa tcctaattta tcacattgat tttatcgcca ctcatattga
    41581 tacgtatcac tgtccctatt cagagtcctg tttcgtatca tatctgtgtt tttttaacgt
    41641 catcacactt ccttcctccc cgaacgctta tcgtcgtttc tggcgtaaat cttgcctgct
    41701 tagactaaat ctttgccata agaaccaaat gttcaaatcg aggggatttc tatggaagcg
    41761 cgggaagcaa ccgctacggg tgaatcatgc atgcgcgtcg atgccattgc taaggtcacc
    41821 gggcgagcac gatatactga cgattatgtt atggcgggca tgtgttacgc gaaatatgta
    41881 cgtagcccta tcgcacatgg ttatgccgta agtattaatg atgaacaagc caggagtttg
    41941 ccgggcgtcc tggcgatttt tacctgggaa gatgtgccag aaatcccatt cgccacggca
    42001 gggcatgcct ggacacttga cgaaaacaag cgcgataccg ccgatcgcgc actgctaact
    42061 cgccatgttc gtcatcatgg cgacgccgtt gccatcgtcg tggcccgcga tgaactcacg
    42121 gcagaaaaag cggcgcaatt ggtcagcatt gagtggcaag aattacccgt tatcacctcg
    42181 ccagaagcgg cgctggcaga agacgctgca ccaatccata acggtggcaa tttactgaaa
    42241 caaagcacga tgtcgacggg taatgtccaa caaacaatcg atgccgccga ctaccaggta
    42301 caggggcact atcagactcc cgttattcaa cattgtcata tggaaagcgt gacatcgctg
    42361 gcatggatgg aggatgactc gcgaattacc atcgtttcca gcacccagat cccgcacatt
    42421 gttcgccgcg tggttggtca ggcgctggat attccctggt catgcgtacg agtcatcaaa
    42481 ccgtttatcg gtggcggttt tggtaataaa caggatgtac tggaagagcc aatggcggca
    42541 ttcctgacca gcaaacttgg cggcattccg gtgaaagttt cccttagccg tgaagagtgt
    42601 ttcctcgcaa cccgtacccg ccacgctttt actattgacg ggcaaatggg cgtgaaccgc
    42661 gacggaacat tgaaaggtta tagtctggat gttctgtcta acaccggcgc ttatgcatct
    42721 cacgggcact ccattgcttc tgctgggggg aataaagtcg cttaccttta tcctcgttgt
    42781 gcctatgctt acagttcaaa gacctgctat accaacctcc cctcggctgg tgcgatgcgt
    42841 ggttatggcg cgccacaagt cgtatttgcc gttgagtcta tgcttgatga tgccgcgaca
    42901 gcgttaggta ttgatcctgt tgaaattcgt ttacgcaacg ccgcccgcga aggagatgct
    42961 aatccgctca cgggaaaacg tatttacagc gcagggttgc cggagtgtct tgaaaaaggc
    43021 cggaaaatct ttgaatggga aaaacgccgt gcagagtgcc agaaccagca aggcaattta
    43081 cgtcgtggcg ttggcgtcgc ctgttttagc tacacctcta acacctggcc tgtcggcgta
    43141 gaaatagcag gcgcgcgcct gttgatgaat caggatggaa ccatcaacgt gcaaagcggc
    43201 gcgacggaaa tcggccaggg tgccgacacc gtgttctcgc aaatggtggc agaaaccgtg
    43261 ggagttccgg tcagcgatgt tcacgttatt tcaacccaag acaccgacgt tacaccattc
    43321 gaccccggcg catttgcctc acgtcagagc tatgttgccg cgcctgcgct gcgcagtgcg
    43381 gcactgttat taaaagagaa aatcatcgct cacgccgcag tcatgctaca tcagtcagcg
    43441 atgaatctga ccctgataaa aggccatatc gtgctggttg aacgaccgga agagccgtta
    43501 atgtcgttaa aagatttggc gatggacgct ttctaccacc ctgaacgcgg cgggcagctc
    43561 tctgccgaaa gctccatcaa aaccaccact aacccaccgg cgtttggctg tacctttgtt
    43621 gatctgacgg tcgatattgc actgtgtaaa gtcaccatca accgcatcct caacgttcat
    43681 gattcgggcc atattcttaa tccgctgctg gcagaaggtc aggtacacgg cggaatggga
    43741 atgggcattg gctgggcgct atttgaagag atgatcatcg atgcgaaaag cggcgtggtc
    43801 cgtaacccca atctgctgga ttacaaaatg ccgaccatgc cggatctgcc acaactggaa
    43861 agcgcgttcg tcgaaatcaa tgagccgcaa tccgcatacg gacataagtc actgggtgag
    43921 ccacccataa ttcctgtagc cgctgctatt cgtaacgcgg tgaagatggc taccggtgtt
    43981 gcaatcaata cactgccgct aacgccaaaa cgattatatg aagaattcca tctggcagga
    44041 ttgatttgag gataacatca tgtttgattt tgcttcttac catcgcgcaa ccacccttgc
    44101 cgatgccatc accctgctgg ctgacaatcc gcaggccaaa ttgcttgccg gtggcactga
    44161 cgtactgata cagcttcacc atcacaatga ccgctatcgc catattgttg atatccacaa
    44221 tctggcagag ctgcggggaa taacacaggc ggaagatggc gcgctgcgaa tcggctccgc
    44281 gacaacattt actcagctca ttgaagatcc cgtaatccaa cgcaatctcc cggcgttatg
    44341 tgctgcggct gcatcaatcg ccgggccgca gatccgtaat gtcgccacct acggcggaaa
    44401 tatttgcaac ggtgccacca gcgcagattc tgccacgcca acgctaattt atgacgcgaa
    44461 actggagctc cactccccac gcggtgttcg tttcgtcccg attaatggct ttcacaccgg
    44521 gccgggcaaa gtgtctcttg agcatgacga aatccttgtc gcctttcatt ttccgccaca
    44581 gccgaaagaa cacgcgggca gcgcgcattt taaatatgcc atgcgcgacg caatggatat
    44641 ttcaacaatt ggctgcgccg cacattgccg actggataac ggcaatttca gcgaattacg
    44701 cctggcattt ggtgttgccg cgccaacgcc gattcgctgc caacatgccg aacagactgc
    44761 acaaaatgcg ccattaaacc tgcaaacgct ggaagccatc agcgaatcag tcctgcaaga
    44821 tgtcgccccg cgttcttcat ggcgggccag taaagagttt cgtctgcatc tcatccagac
    44881 gatgaccaaa aaagtgatta gcgaagccgt cgccgcggcg gggggaaaat tgcaatgaat
    44941 cacagcgaaa caattaccat cgaatgcacc attaacggga tgccttttca gcttcacgcc
    45001 gcgccaggaa tgccgctttc ggaactactc cgagaacaag ggcttcttag tgtcaaacaa
    45061 ggttgctgcg taggcgaatg cggtgcctgt acggtgctgg tcgacggcac tgcgatagac
    45121 agttgcttat tccttgcggc ctgggctgaa ggaaaagaga tccgcacgct ggaaggtgaa
    45181 gcgaaaggcg gtaaactttc tcatgtccaa ctggcttatg cgaaatctgg tgcagtgcaa
    45241 tgcgggtttt gtacgccggg cctgattatg gctaccacgg caatgctggc gaaaccacgc
    45301 gagaagccat taaccattac ggaaattcgt cgtggactgg cgggaaatct ttgtcgctgc
    45361 acagggtatc agatgattgt aaatacagtt ctggattgcg agaaaacgaa gtaaaaggat
    45421 atccggcctg aattcaggcc ggattctcag aggttatgtg tttaacaact catatttctt
    45481 aatcttgcga tagagcgtag caatgccgat gcccagttca tcagcaactt ccttcttgct
    45541 gttatgacgt gaaagcgcct cgcggatcat ttgcttttcc atctcctcca gcgccgtgcc
    45601 gcccgcatca tcgagtgaca ggtgcgcctc actgacctct gttacatcac tttgctccgt
    45661 tgtgccatta ttcagcagat ttggcggcaa tagcgtgctg tcgataactt cacctgaagg
    45721 aacaacgtta accagatatt ccatcaaatt gcttaactcg cgcaggtttc cgggccaacg
    45781 atgcttacgc aatatttcga cgacatcagg agcaatgcca ggataaaccg atcccagacg
    45841 acgggtatgc agatgtaaaa agtaatgcac cagtaattca atatcttcct gacgttcacg
    45901 cagcggtgga agagttatcg ggataacatt aagtcggtag aagagatctt cgcggaattt
    45961 accttcggca atgaactggg ccaaattctg attagttgca gaaatgatgc ggatgtcgac
    46021 ttgtattggg ctactggcac caatcggcag aatttcacgc gcctcaatag cgcgcagtaa
    46081 tttagcctgc aacattaatg gcatatcacc aatttcatcg agaaacagcg tgcccgtatt
    46141 cgccgcctga atcaaccctg ttttaccgtt ggcagaagcg ccagtaaatg cgcctttaac
    46201 ataaccgaac agttcgctct ccagaagctg ttccggaatc gcggcacagt tgatagcaat
    46261 aaagggttta ttccgtcttc cgctcaactt atggattgca cgagcgacga cttctttacc
    46321 cgtgccgctt tcaccaacca ccataacgct ggatgggctg ggtgcaatac ggctaatgag
    46381 tcgttttaat tgccgcataa cacggcactc gccaaccaat tgttcaatat gcggttcatc
    46441 aggtgcattt gctacagaaa aactggtatg cgattggtga aacgccatta aaaatagttg
    46501 tcggccctgg atgttatgca actgaccaat gattaattca cttttatcat cccacgaaac
    46561 aatatgttgc atatgaccat gggtaaaatt actctcaaat gttaatggtc tgaaacgaac
    46621 aggttttcca ataatattat tttgtacaac accgagtgtt tttaaagctg tctgattaac
    46681 aaactgaacc cgattatcag catcaaccac taacacgccc tgatccatat tatcgatcat
    46741 ggtcgcgaat attttactga tgttatctcc tggtccctga tcctccagaa gtttagaaac
    46801 aaaaatggtg gatatatggc gaacgtaatc ggaaaattcg cgtaaattat cactgatatg
    46861 ttcttgttgc tcgtgggtaa cggcaatcaa acttatcacc ccaacacaac gatcctgtaa
    46921 aatgacaggc gtacccagaa atgctttttc gcggcaattt tctttactat cgcaaccttc
    46981 acaaagggga tcgaagcgag actgtgtcac aactttttca gttttcgttt ccaggacgtg
    47041 gcggagcagg cgtgagttgc cgctcaactg gcggccaaga aacttcccat acgcgcccgt
    47101 tccggcgacg cgacacaagt tttcatcaac gatctcaacc tcaagctgca aaacgctggc
    47161 aagcattctg gcaaaacgct gaattgtcgg ttgaatttgc atcaatactg actgcgtagt
    47221 cgcaagctcc atagctttac cttccagact tacttaaaag tcgatcattg aagacgttga
    47281 tggttcacag atcatgatga tattaactca ggcgaaattg gctttgataa aaacataaga
    47341 tttttatcac tttctaataa aattatggaa gagatatcac atttctatat caatatgaga
    47401 attacagcgg caagtttatc aaactgaaga gagatagcct gcctctttat cttatttctg
    47461 atacttagca gcaaataaat aacgtgataa aaaaagccaa acgttttcgt attttacaaa
    47521 caaccggaaa ctggcatcaa tatgtgatca accccacaca ttatccgtca aattagcctt
    47581 tcgcagccgc gtggataatt ctggcacact tattgttagt cccaggtata gctgtgaaaa
    47641 caccaatcac tttggcacgt cacagtgaaa taaaccactt tgcctgtcat tccactatcg
    47701 ggactttatg atgaaaactg ttaatgagct gattaaggat atcaattcgc tgacctctca
    47761 cctgcatgaa aaagattttt tgttaacgtg ggaacagacg ccagacgaac tgaaacaagt
    47821 actggacgtt gccgccgcgt taaaagcact gcgtgctgaa aacatctcaa ccaaagtctt
    47881 taatagtgga ttaggtattt ccgtcttccg cgacaactcc acccgtaccc gcttctctta
    47941 tgcttccgcg cttaacctgc tcggccttgc acaacaagat cttgacgaag gtaaatcaca
    48001 aatcgctcac ggcgaaaccg tgcgtgaaac cgccaatatg atctccttct gcgctgacgc
    48061 tatcggtatt cgcgacgata tgtatctggg cgcaggcaac gcctatatgc gtgaagttgg
    48121 cgcggcactt gatgacggct acaagcaggg tgtgctgccg cagcgtccgg ctttagtgaa
    48181 cctgcaatgc gatattgacc acccgactca gtctatggct gacctggcat ggttgcgtga
    48241 acactttggt tcgctggaaa acctgaaagg caaaaaaatc gccatgacct gggcctactc
    48301 cccaagctat ggcaaaccgc tctctgtacc acaaggcatc ataggtctga tgacccgctt
    48361 cggtatggat gtcaccctgg cccatccgga aggctacgac ctgatcccgg atgtcgttga
    48421 agttgctaaa aacaatgcta aagcctccgg tggtagcttc cgtcaggtca ccagcatgga
    48481 agaagccttc aaagacgcag acatcgttta tccgaagtca tgggcacctt acaaagtgat
    48541 ggaagagcgt actgaattgc tgcgtgcgaa cgatcacgaa ggcttaaaag cactggaaaa
    48601 acagtgtctg gcacagaacg cgcaacacaa agactggcat tgtactgaag agatgatgga
    48661 actgacccgt gatggcgaag ccctgtacat gcactgcctg ccagctgata tcagcggcgt
    48721 atcctgcaaa gaaggtgaag tgactgaagg cgtattcgaa aaataccgta tcgctaccta
    48781 caaagaagcc agctggaagc cttatatcat cgccgcgatg atcctgtccc gtaaatacgc
    48841 caaaccaggt gcactgctcg agcaactgct gaaagaagcg caagaacgcg tgaaataacc
    48901 cctcgggccg atcagctggt cggccctcta tttccagagg ccaaaaggat aggatatgtc
    48961 cgttttctca ttgaagattg atatcgccga taacaaattt ttcaacggcg aaacatcacc
    49021 gcttttttcg caaagccagg caaaactggc acgccagttc caccagaaaa ttgctggtta
    49081 tcacccaaca ccgctttgtg cgctggacga tctcgcaaac ctgtttggtg taaagaaaat
    49141 tctcgtcaaa gacgaatcaa aacgattcgg tctgaacgcc ttcaaaatgc tcggcggtgc
    49201 gtacgccatc gctcaattat tgtgtgaaaa atatcatctt gatatcgaaa cgctgtcatt
    49261 tgagcacttg aaaaatgcca tcggcgaaaa aatgactttc gcgactacca ccgacggcaa
    49321 ccacgggcgc ggtgtggcat gggcagcaca gcaactcgga cagaatgcgg tgatttacat
    49381 gccgaaaggt tctgctcagg aacgcgttga cgccattcta aacctcggtg ccgagtgcat
    49441 cgtcacggat atgaactatg acgataccgt tcgcctgacc atgcaacacg cgcagcagca
    49501 cggctgggaa gtggtacagg acacggcatg ggaaggttac accaaaatcc caacctggat
    49561 catgcaaggc tacgcaacac tggcggatga agccgtcgag caaatgcgtg aaatgggcgt
    49621 caccccgaca cacgttctgc tgcaagccgg tgtcggtgca atggccggtg gtgtgctggg
    49681 ttatctggtc gacgtctata gcccgcaaaa tctgcacagc attattgttg aacctgacaa
    49741 agctgactgt atttatcgct ccggcgtcaa aggcgacatc gtcaacgttg gcggtgatat
    49801 ggccaccatc atggcgggcc tggcctgcgg cgaacctaac ccactgggct gggaaatcct
    49861 acgtaactgc gccacccaat tcatctcctg ccaggacagc gttgccgcat taggtatgcg
    49921 cgtgctgggt aatccgtatg gcaacgaccc gcgcatcatc tctggtgaat ccggcgctgt
    49981 cggtttgggc gttctcgcag cggttcatta tcacccgcaa cgtcaaagcc tgatggaaaa
    50041 actggcgctg aacaaagatg ccgtggtgct ggttatcagc actgaaggcg acaccgacgt
    50101 gaagcactac cgcgaagttg tctgggaagg caaacacgct gtagcacctt aatcacccta
    50161 ttggaactgc tccctgcaaa acggggagta aaaaatctgg agaaaaataa tggctaagaa
    50221 tattccattc aaactgattc ttgaaaaagc aaaagattac caggcagaca tgactcgctt
    50281 cctgcgcgat atggttgcta ttcccagtga aagctgcgac gagaaacgcg tagtacatcg
    50341 tattaaagaa gagatggaaa aagtcggctt cgataaagtt gaaatcgacc caatgggcaa
    50401 cgttctcggt tatatcggcc acggcccgcg tctggtggca atggacgctc atatcgatac
    50461 agtcggcatt ggcaacatca aaaactggga cttcgatccg tatgaaggca tggaaaccga
    50521 cgagctgatc ggtggtcgtg gtacttccga ccaggaaggc ggcatggcgt ctatggtata
    50581 tgccggaaaa atcattaaag atctcggtct ggaagatgaa tataccctgc tggtcaccgg
    50641 tactgtgcag gaagaagact gcgacggtct gtgctggcaa tacattattg aacaatccgg
    50701 cattcgcccg gaatttgtgg tcagtaccga accaaccgac tgccaggtat accgtggtca
    50761 acgcggtcgt atggaaattc gcattgatgt tcagggtgtt agctgccacg gttcggcacc
    50821 agaacgtggt gataacgcca ttttcaaaat gggtccaatt cttggcgaat tacaggaact
    50881 gtctcaacgt ctgggctatg acgaattcct cggcaaaggt acgctgactg tttctgaaat
    50941 cttcttcacc tccccaagtc gttgcgctgt agcagacagc tgcgcagttt caatcgaccg
    51001 tcgtctgacc tggggcgaaa cctgggaagg cgcgctggac gagatccgcg ctctacctgc
    51061 agtacagaaa gctaacgcgg ttgtttctat gtacaactac gaccgcccgt cctggactgg
    51121 cctggtttac ccaaccgaat gctacttccc gacctggaaa gtggaagaag atcacttcac
    51181 cgttaaagca ctggtgaatg cttatgaagg gctgtttggt aaagcgcctg tcgttgataa
    51241 gtggaccttc tcgactaatg gcgtatccat catgggtcgc cacggcattc cggtcatcgg
    51301 ttttggccct ggtaaagaac cggaagcgca cgcgccaaac gaaaaaacct ggaaatctca
    51361 cctggtaacc tgtgccgcta tgtacgctgc aatcccatta agctggctgg caaccgaata
    51421 attactcttc acttattccc ctccggttcg ccggagggtt tttggagttt gctatgcgcg
    51481 tattgataaa aaacggcatt gttgttaatg cagatggaca ggcgaagcag gatttgctga
    51541 ttgaaagcgg gattgttcgc cagttgggta ccgatatttc gccgcaactc ccttgcgaag
    51601 aaattgatgc ctctggctgt tacgttttcc ctggcggcgt ggatgtccat acgcatttca
    51661 atattgatgt cggcatcgcg cgcagttgtg atgatttttt taccggtacc cgcgctgctg
    51721 cgtgtggcgg tacaacaacc attattgacc atatgggatt tggcccaaat ggctgccggt
    51781 tacgccatca actggaagtt tatcgcggtt atgccgccca caaagcagtc atcgactaca
    51841 gctttcacgg tgtcattcaa catattaatc acgctatcct cgacgaaatt ccgatgatgg
    51901 ttgaggaagg actgagcagt tttaaactct atttaaccta tcaatataaa ctcaacgatg
    51961 atgaagtgtt gcaggcatta cgccgcttgc atgagtccgg cgcgctgacc accgtacacc
    52021 cggaaaatga cgcagctatc gccagcaagc gggcggagtt tatcgccgca gggttaaccg
    52081 cgccgcgtta tcatgccttg agtcgacctc tggaatgcga agcggaagcc atcgcccgca
    52141 tgattaacct ggcacaaatt gccggtaacg ccccgctcta tatcgtgcac ctgtctaacg
    52201 gcttaggtct ggattatctg cgtcttgccc gtgcgaatca ccagccagtc tgggttgaaa
    52261 cctgcccaca atatctcctg ttggacgaac gcagttacga tacagaagat ggcatgaagt
    52321 tcattcttag cccaccgctg cgtaacgtac gcgagcagga caaactgtgg tgtggcatca
    52381 gcgatggtgc gattgacgtg gtggcaaccg atcactgcac cttctcgatg gctcaacgcc
    52441 tgcaaatttc taaaggcgat ttcagccgct gcccaaatgg cttacccggt gtggagaacc
    52501 gcatgcaact gctgttctcc agtggcgtga tgacgggacg tatctcaccg gagcgttttg
    52561 ttgagttaac cagtgcaatg cccgccaggc tgtttggcct gtggccgcaa aaagggatat
    52621 tagcgcccgg ttccgatggt gacgtggtga ttatcgaccc acgtcagagc caacaaattc
    52681 agcatcgcca tctccacgac aacgctgact actcgccatg ggagggtttt acctgtcagg
    52741 gcgcgattgt cagaacctta tcccgtggtg aaacgatttt ctgcgacggc acctttacag
    52801 gcaaagccgg gcgaggtcgt ttcctgcgac gcaaaccgtt tgtccctccc gtgctctaac
    52861 cactgccagg gatctataca caatcaagaa tgatgagtga ggaaaaatga gtaagaaaat
    52921 tgttctcgcc ctgggcggga atgcgctggg cgatgacctg gccggacaaa tgaaagcggt
    52981 aaaaattact tctcaggcaa ttgttgattt aattgcccag ggacatgagg tcatcgtcac
    53041 tcatggcaac gggccacagg tgggcatgat taaccaggcc tttgaagctg ccgcaaaaac
    53101 tgaagcgcac tcgccaatgc tgccgatgtc tgtctgtgtt gccttaagcc agggttatat
    53161 tggttacgat ctacaaaacg ccttaagaga agaactgctt tctcgtggca ttaataaacc
    53221 tgtagcaacg ctggttaccc aggtcgaagt cgatgctaac gatccggcat tcctcaaccc
    53281 gaccaagccg atcggttcgt tctttaccga gcaagaagca gagctactga caaaacaggg
    53341 ttacactctg aaagaagatg cgggtcgcgg ctatcgtcgt gtcgttgctt cgccaaaacc
    53401 ggttgatatc attgaaaaag aaacggttaa agctctggta gatgccggac aggtggtgat
    53461 caccgttggc ggtggtggta tccctgttat tcgtgaaggt aaccatctgc gtggtgccag
    53521 cgcggttatc gataaagact gggccagcgc ccgtttagca gaaatgatcg atgccgatat
    53581 gctgatcatt ctgactgcgg tagaaaaagt ggccattaac ttcggaaaag agaatgaaca
    53641 gtggctcgat cgcctgtcgt taagtgatgc tgaacgcttt atcgaagaag gtcattttgc
    53701 gaaaggttct atgctgccta aagtggaagc tgccgcctcg tttgctcgct ctcgcgcagg
    53761 tcgtgaagca ctgattaccg tattgagcaa agcgaaagaa gggattgaag gcaaaaccgg
    53821 aacggtgatt tgtcagtaat ttaaaggccg gatgggtaat accatccggc cattttcatt
    53881 taagccactt ccagcacttc ttttgtcgct ttaacgccct gatgcatcaa catcattaac
    53941 gcctcaagta cgccgccgcc aatcgcccgg gctttatcag aaacgctggt gaaatcagcc
    54001 gtttcaccac gaggatcgat atcaccaatt ttaaaaccac cgaccacggc cagcccgtcg
    54061 ttcaacaagc cacgcaccat ccccgtcaac ggtgctttaa tttcatgctc accaatccag
    54121 gcaatgacat cgccctcttt caccagatcg cctaatttca cgttggatcg cataatgcct
    54181 gcagcaggag cgcggatcac tcgtcgggtg gtatgcccca taatattgcc gggaacaccg
    54241 gtattctcct gcgcacaacc tgagtaaatc acctgaccga gccagtgccc gcgatttgtt
    54301 tcaattaccg catgacaatc cttccctgca gtaaagcccg gcccaagcgc gattgttact
    54361 ggtgccatat ctgcccgcgt acccaaattc tgtttcgcca gaatagcgtc caccacgcaa
    54421 agcggtttca gttcatcaag caatgaacag gtgggatcta ccatcacagg gatgaatccg
    54481 cgttcggtaa gtttcatcgc ttccgcagag ctggttgcca ggcgggcggt gacgccttcg
    54541 accgtcattt cgccatcgaa cacggcctgg gcaaacgcca cggtacaacg aatcactgtc
    54601 ggtttttcca cttccaacat gatcacttta aaacccgcat gatacagacg tagcgctaca
    54661 ccactggcga tatcccccgc accgcgaatc accaccagct ggtgtccgga aggatgagaa
    54721 ggcttcatca tcagcccacc cggcgcgtta tttttcacct gtaatatttc tgccagcacg
    54781 ctgatggcga tctcctgcgg cgtttccgca cctatgttgt aaccaacggg cgcatgtaaa
    54841 cgggcaatat gttcttcagc cacgcctttc tcacgcaatt ggcgcaggaa aagctgaacc
    54901 ttgcggcgac ttgccagcaa ccccagccat gcaatgggtt gttcaatgag tttgtcgagg
    54961 gcttcacgat cctgattatt cgtggcaatc aggacaaaat tatcagggcg aatatccagt
    55021 gcttccactg ccgcaccaaa cgactcagca tggagaagcg tggttgatgg tgggaatagt
    55081 tcaggattga ggctttcgcg ataaatatca gcaacggcga tatcaaatcc taatagcgcc
    55141 gcactctggg ctatcgcccg gttgacatgc cccgcaccaa tcaacaccag acgtggacgc
    55201 ataccatgga cgctgataaa tactgacatt gcacctccac aatctgaccc gacagcatcc
    55261 gcaccgttac gagccatacg tccatggaat aatcgcggct tacgttcctg caatgcctga
    55321 agcgactctt caatcacctt ccgctcaacc attccgccac caattgtacc gacgatagaa
    55381 ccatcggcgc gcactaacat ttgtgcagaa tggcgggggg ttgagcctcg gctatcaaca
    55441 atttgcgcca tcgcaaacgg acaattttgc tcttcgagtt ttgcagcctc tgtgaaaata
    55501 ttcataccaa cctcaatgga tatcctttca gtaacccgga atacccgggc cgctaattca
    55561 caaatctgcg cgcgattgca ggatgttctt gtatatcgcc cagccagatt gcctctacgt
    55621 tgtgttgttg cagcggttga aggagctcgc tttgcgcaat cgcattctca cattgagaaa
    55681 aacggttaat gaaccagact cgccgacaac cttgtggtac gtttttaaac gccccctgag
    55741 ggtggcgaac cagcgcaacg agatcgctca gttgcaaggg tgcagcaggt gttaacccag
    55801 taatatcagc aaactgcgac cagcgatgga cattttctgt gctcactttc gcacccaaaa
    55861 tatgtcctcc catcacagca atcacgcagc aactgctttt aggtatgcaa ggttcgtgct
    55921 catcaggcgc ttttaacggc attccacgcg agccatctgc ctcaatgaga attacgtcac
    55981 actctggtcg ttgtaccagt gcatcaatcg cttctggcgt aaatccctgc acttttccct
    56041 ggttcgcttt ccagcagtga aaacaaaatg aaatggggga tataaaagac gcatgaggaa
    56101 gcatggcggg atcacgacag aaaaccacgg gccagtgaga tgttggcata aacatatgtg
    56161 tcgtcgtagt gattaataca cgcctgccac tcgcctggaa cagttctgcc agccaaaaaa
    56221 gcaagctggt tttcccccca gcaccaacaa tagaaatcac agtgggacgt ttctgcgcgc
    56281 ctaaatcaat gaataatgcc gatgggtcaa ttatactttt cacgcgtctt tatggacctc
    56341 tctgggataa attaccctca acgtaatcca tcaggaagta acgcaattat caggcgttat
    56401 tagctcctgt aaataatgga atcactatgt cagccatcga ctgtataatt accgccgcag
    56461 gattatcatc aagaatgggg caatggaaaa tgatgttacc ctggcaacag ggaacaattc
    56521 ttgatacaag tatcaaaaat gcgttgcagt tttgtagccg aattatttta gtcaccggct
    56581 atcgtggtaa tgaactgcac gaacgttatg tgaaccagag caatatcact attatccaca
    56641 acccagatta tgcgcagggt ttactgacat cagtaaaggc cgcagtaccc gcggtgcaaa
    56701 cagaacattg ttttctcacc cacggagata tgccaaccct caccatcgat atttttagaa
    56761 aaatctggtc gttacgaaat gatggcgcaa tactgccact ccataatggc atccccggcc
    56821 atccgatttt agtgtcaaaa ccatgcctga tgcaggcaat ccagcgaccc aatgtcacca
    56881 atatgcgtca ggcactcctt atgggagaac attattccgt cgaaatagaa aatgcagaaa
    56941 taattttaga tattgatact ccggacgact ttattacagc gaagaaaagg tatactgaaa
    57001 tttagaaaac gtagttaaac gattgcgttc aaatatctaa tccctccagc attctttatc
    57061 ataatgataa ggaatcactg attttgagaa aagagaaaaa gtgataaatg ggctggcaat
    57121 aataattttc aggagtgatt ttaattctca tttggcacct gtgagttcat tcacaaaaag
    57181 ccattaagtt tcgagccgcc tcacattttt ttatatttcc cgccaaacct ggcaagagtg
    57241 gtgcgattgt tgctctatcc ccctaaacca ccggatttct caacaccggt cactcaatga
    57301 tatctgtata agctaaggag agggttatgg gggatattat gcgtcccatt ccgtttgagg
    57361 aacttttgac gcgcatattt gatgaatacc aacaacaacg ctcaatcttt ggtattcccg
    57421 agcaacagtt ttactcaccc gtaaaaggta aaactgttag cgtcttcggt gaaacctgtg
    57481 ccactcccgt cggccctgcc gctggcccgc acacgcaact tgcgcaaaac atcgtcacct
    57541 cctggctgac tggcggacgc ttcatcgaac taaaaaccgt ccaaattctt gaccgcctgg
    57601 agctggaaaa gccctgtatc gatgccgaag acgagtgctt taacaccgaa tggtctaccg
    57661 aatttaccct gcttaaagcc tgggatgaat acctcaaagc ctggtttgcc ctgcaccttc
    57721 tcgaagcgat gttccagcct tctgattccg gtaaatcgtt catctttaat atgagcgtcg
    57781 gttacaacct cgaaggtatt aagcaaccgc cgatgcagca gttcatcgac aatatgatgg
    57841 acgcatctga ccatccgaaa ttcgctcaat accgcgatgc acttaataag ctactccaga
    57901 atgaggcatt tttagcccgc cacggattgc aggaaaaacg cgaaagcttg caagccttac
    57961 ccgctcgcat ccccaccagc atggtacaag gggttaccct ctccaccatg cacggctgtc
    58021 ctccgcatga aatcgaagcc atttgccgct acatgctgga agaaaaaggg ctcaacacct
    58081 ttgtgaaact caacccgacc ttactggggt acgcgcgtgt tcgtgagatc ctcgatgtct
    58141 gcggtttcgg ttacatcggc ttaaaagaag agtcatttga tcacgacctc aagctgacgc
    58201 aagcactgga aatgctggaa cgcctgatgg cactggcaaa agaaaaatca ctcggctttg
    58261 gcgtaaaact gactaacact ctcggcacca tcaacaataa aggcgcattg cccggtgaag
    58321 agatgtatat gtcaggccgt gcgctgttcc cgctctccat caacgttgca gcagttctct
    58381 ctcgcgcctt tgacggcaaa ctgcccattt cttattccgg tggtgccagt cagctgacta
    58441 tccgcgatat ttttgatact ggtattcgcc ctattactat ggcaaccgac ctgctgaaac
    58501 ctggcggcta tctgcgctta agtgcctgca tgcgcgagct ggaaggctca gatgcctggg
    58561 agcttaacca cgttgacgtc gaacgactga acagactggc agcagacgcg ttaaccatgg
    58621 aatacaccca gaaacactgg aagccagaag agcgtattga agtggctgaa gacctgccgc
    58681 tgaccgattg ctacgttgcc ccctgtgtta ctgcctgcgc tatcaagcaa gatattccgg
    58741 aatacatccg tctgcttggc gaacaccgct atgccgacgc gctggaactc atctatcaac
    58801 gcaacgccct gcccgccatt accggtcata tttgcgatca ccagtgccaa tacaactgta
    58861 cccgcctgga ttacgacagt gcgctgaata tccgcgaact gaaaaaagtc gcgctggaaa
    58921 aaggttggga tgaatataag caacgctggc acaaaccagc cggttctggt tcacgccatc
    58981 cggttgccgt gattggtgca ggtccggcgg gtctggcagc aggttacttc cttgccagag
    59041 cgggccatcc ggttacgctg tttgaacgcg aagccaatgc gggcggcgtg gtgaaaaata
    59101 tcattcctca gttccgtatt cctgcagagt taattcagca cgatatcgat tttgttgccg
    59161 ctcacggcgt aaaatttgag tatggctgct caccggatct gaccgttgaa cagttaaaaa
    59221 atcagggctt ccactatgtt ctgattgcca ccggcactga taaaaatagc ggtgtgaaac
    59281 tggcgggcga caaccaaaat gtctggaaat cactcccctt cctgcgtgaa tacaacaagg
    59341 gcacagcgct caagctgggc aaacatgtgg tcgttgtcgg ggcgggtaac acggcaatgg
    59401 actgcgctcg tgcggcgtta cgcgttccag gcgtagaaaa agcaacggtc gtttaccgtc
    59461 gttcactgca agagatgcca gcatggcgcg aagagtatga agaagcgttg cacgacggcg
    59521 tggagttccg tttcctgaat aatccggaac gtttcgatgc tgatggcacc ttaaccttgc
    59581 gcgttatgtc gcttggcgaa cctgatgaga aaggtcgtcg ccgtccggtt gaaaccaacg
    59641 aaacagtaac actgcatgta gacagcctga tcaccgccat tggtgaacag caggatactg
    59701 aagccctgaa tgcgatgggc gtgccgctgg acaaaaacgg ctggccagac gtcgaccata
    59761 atggcgaaac gcgtctgagt gacgtcttta tgatcggcga cgtacagcgc ggaccatcct
    59821 ccattgtcgc tgctgtcgga accgcgcgtc gggcgaccga taccatcctg agtcgggaaa
    59881 atatccgttc ccaccagaac gataaatact ggaataacgt caatccggcg gaaatctatc
    59941 aacgtaaagg cgatatctct gtcactctgg tgaacagtga cgatcgtgat gcgtttgtcg
    60001 cccaggaagc cgcacgctgc cttgaatgta actacgtttg cagcaagtgt gtggatgtct
    60061 gcccgaaccg cgccaacgta tccattgcgg tccctggctt ccagaaccgt ttccagacgc
    60121 tgcacctcga cgcttactgt aacgaatgcg gcaactgcgc tcagttctgt ccgtggaacg
    60181 gtaaaccgta caaagacaaa atcaccgtct tcagcctgtc gcaagacttt gataacagca
    60241 gcaaccctgg cttccttgtg gaagattgcc gggtacgcgt acgtctgaat aaccaaagct
    60301 gggtgttaaa catcgacagc gaaggtcagt tcaacaacgt accaccggag ctgaacgata
    60361 tgtgccgcat cattagccat gtccaccagc atcatcatta tctgctgggc cgcgtggagg
    60421 tgtaatcatg ttgattctga agaatgtcac cgcagtgcag ttacacccgg cgaaagtgca
    60481 ggaaagcgtt gatatcgcca tcgaaaatga tgtgattgtc gctatcgacg atgccctgac
    60541 gcaacgctat cccgatgcca gctacaaaga gatgcatggt cggattgtga tgccgggaat
    60601 tgtctgctcg cacaaccatt tttactcggg gctttcccgc ggaattatgg caaacatcgc
    60661 cccctgcccg gatttcatct caacgctgaa aaatctctgg tggcggctcg atcgcgccct
    60721 tgatgaagag tcgctctatt acagcggact gatttgttcc ctggaagcaa ttaagagcgg
    60781 atgtacatcg gttatcgatc accatgcctc tccagcctat atcggcgggt cgctctccac
    60841 attgcgcgac gcatttttaa aagttggcct gcgcgcgatg acctgttttg aaactactga
    60901 ccgtaacagc ggcatcaaag agttgcagga aggtgtagaa gaaaacatcc gcttcgcccg
    60961 tcagattgat gaggcgaaga aagcagcaac cgagccgtat ctggtggaag cacatatcgg
    61021 cgctcacgcg ccgtttaccg taccggatgc cggtctggag atgctgcgtg aagccgtgaa
    61081 aagcacaggt cgtggtttgc atattcacgc tgcggaagac ctttacgacg tttcctacag
    61141 tcaccattgg tacggcaaag acctgctggc acgactggcg caattcgatc tcatcgacag
    61201 caaaacgctg gtcgctcatg ggctgtactt gtcgaaagat gacatcgccc tactcaatca
    61261 gcgcgatgcg ttcctggtgc ataacgcccg ttcaaacatg aacaaccatg tcggctacaa
    61321 ccatcacctt agcgacatcc gcaatctggc gttgggaacg gacggcattg gttcggacat
    61381 gtttgaagag atgaaatttg ccttctttaa acatcgcgat gcgggtggcc cgctgtggcc
    61441 tgacagtttt gccaaagcac tggctaacgg caacgaactg atgagccgca actttggcgc
    61501 gaaatttggg cttctggaag ccggttacaa agccgattta accatttgcg attacaactc
    61561 gccaacgccg ctgctggcag acaatatcgc cgggcatatc gctttcggta tgggctcagg
    61621 cagcgttcac agcgtgatgg tcaatggtgt gatggtctat gaagaccgtc agtttaactt
    61681 cgattgcgat tccatttatg cgcaagccag aaaagccgct gccagtatgt ggcgtcggat
    61741 ggatgcgctg gcataaataa cagctcccct cttctttcag agaagagggg aaattaagga
    61801 aagacgatga ttgaacaatt tttcagaccc gactctgtcg aacaggcgct ggaactgaag
    61861 cgccgctacc aggatgaagc cgtctggttc gccgggggca gcaaactcaa cgctacacca
    61921 acccgtaccg ataaaaagat tgccatttcc ttgcaggatc tggagctgga ctggattgac
    61981 tgggataacg gtgcactacg aattggcgca atgtctcgct tgcagtcact gcgtgatgcg
    62041 cgatttattc ctgcagcact gcgggaagct ctcggttttg tctactctcg ccatatccgt
    62101 aatcagtcga ccattggtgg tgaaatcgcc gcccggcagg aagagtcggt gctgcttccc
    62161 gtcctgctgg cactggatgc tgaactggtt tttggcaacg gcgaaacgct gtcaatcgag
    62221 gactacctgg cctgcccatg cgatcgcctg ttaaccgaaa ttatcattaa agatccgtat
    62281 cgcacctgtg cgactcgcaa aattagccgt tctcaggcag gtttaaccgt cgtgacggca
    62341 gccgttgcaa taacagacca cgatggtata cgaattgcgc tggatggcgt cgccagtaaa
    62401 gcactgcgtc tgcatgatgt tgaaactcaa aatctggaag gcaatgcact tgaacaggct
    62461 gtcgccaacg ccattttccc gcaggaagat ttgcggggca gcgtggccta taaacgctat
    62521 atcacgggag ttctggtagc cgacctgtat gccgactgcc aacaggctgg ggaggaagcc
    62581 gtatgatcat ccactttact ttaaatggcg cgcctcagga gctaaccgtt aatccaggcg
    62641 aaaacgtgca aaagctgttg tttaacatgg gaatgcactc tgtacgcaac agtgatgatg
    62701 gttttgggtt tgccggttct gacgcaatta tctttaacgg taatatcgtt aacgcgtcct
    62761 tgcttatcgc cgcacagtta gagaaggcag atattcgtac cgcagaatct ctgggcaaat
    62821 ggaacgagtt aagtctggtt caacaggcca tggttgatgt tggcgtggtg cagtctggtt
    62881 ataacgatcc aactgcggct ctgattatca ccgatcttct cgatcgcatc gacgcaccta
    62941 cccgcgaaga gatcgacgac gcgctctcag gtctgtttag ccgcgatgct ggctggcagc
    63001 aatactatca ggtcattgaa ctggcggttg cacgtaaaaa taatccgcag gccaccattg
    63061 atatcgctcc gactttccgt gacgaccttg aagtcattgg caagcattat cctaaaactg
    63121 atgccgcgaa aatggtgcag gcaaaaccct gctatgttga agaccgcgta acggctgacg
    63181 cctgcgtcat taaaatgtta cgtagcccac acgctcacgc actgattact catctggatg
    63241 tcagcaaagc tgaagcctta ccgggcgtcg ttcacgttat tactcacctg aattgcccgg
    63301 atatttacta taccccgggt ggtcagagcg caccggaacc atcaccgctt gaccgccgta
    63361 tgttcggcaa gaaaatgcgt cacgtcggcg atcgcgttgc tgcggtagta gccgaaagtg
    63421 aagacattgc gctcgaagca ctgaagctca tcgatgttga atatgaagtg cttaagccgg
    63481 taatgtcgat tgacgaggca atggcggaag atgcgcctgt cgtgcacgat gaaccggtgg
    63541 tgtatgttgc tggtgcacca gatactctgg aagatgataa ccgtcatgca gcccagcgcg
    63601 gcgagcatat gatcatcaac ttcccgatcg gttctcgccc acgcaaaaat atcgctgcca
    63661 gtattcatgg tcatattggc gatatggaca aaggctttgc cgatgccgat gtgatcattg
    63721 agcgaaccta taactcaacg caggcgcagc agtgcccgac tgaaacacat atctgcttta
    63781 cccgtatgga cggcgatcgt ctggtgatcc acgcctccac ccaggtacca tggcacttac
    63841 gccgccaggt cgcgcgcctc gtgggcatga aacaacataa agttcatgtc attaaagaac
    63901 gtgtaggcgg cggttttggc tctaaacagg acatcctgct ggaagaagtg tgcgcctggg
    63961 caacttgcgt gaccgggcgt ccggtacttt tccgctacac ccgtgaagaa gagtttattg
    64021 ctaacacctc tcgtcacgta gcgaaagtca ccgtcaaact gggcgcgaaa aaagatggtc
    64081 gcctgacggc agtgaagatg gatttccgcg ccaacactgg tccttacggt aaccactcac
    64141 tcaccgtacc gtgtaacgga ccggcgctgt cgctgccgtt atatccatgc gataacgtcg
    64201 atttccaggt caccacctac tacagcaaca tttgcccaaa tggtgcttat cagggttatg
    64261 gcgcaccgaa aggtaacttc gctatcacca tggcgttagc ggaactggcg gaacaattgc
    64321 agatcgacca actggaaatt attgaacgta atcgtgtaca cgaagggcag gagttgaaaa
    64381 ttctcggtgc gattggtgaa ggtaaagcgc cgacatccgt tccttccgcc gccagctgcg
    64441 cactggaaga gatcctgcgt cagggtcgcg agatgatcca atggtcttca ccaaaaccgc
    64501 aaaatggtga ctggcacatc ggtcgcggcg tcgccattat catgcagaaa tcagggatcc
    64561 cagatatcga tcaggctaac tgcatgatca aactggaatc agacggtacc tttatcgttc
    64621 attctggcgg tgcggatatt ggtactggtt tggatactgt ggtgacgaaa ctggcagcag
    64681 aagtgctgca ctgccccccg caggacgtgc atgttatctc cggtgatacc gatcatgcgt
    64741 tgtttgataa aggcgcatat gcctcgtccg gtacttgctt ctcgggtaac gctgcgcgtt
    64801 tggcagcgga aaatctgcgg gagaaaatcc tgttccacgg cgcgcaaatg ttgggtgagc
    64861 cagtggcaga tgttcaacta gcaacgccgg gcgtcgttcg cggcaagaaa ggcgaagtta
    64921 gtttcggaga gatagcccat aaaggcgaaa ccggcaccgg ctttggttca ctggtgggaa
    64981 ctggcagtta tatcacgcct gatttcgcct tcccgtatgg cgcaaacttc gctgaagttg
    65041 ccgtcaacac gcgtacgggt gaaatccgcc tggataaatt ctacgccttg ctggactgcg
    65101 gtacaccggt caatccagag ttagcgctgg gacaaatcta cggtgccacc ctgcgtgcta
    65161 tcggccacag tatgagcgaa gagatcattt atgacgccga aggtcacccg ttaacgcgtg
    65221 atttacgcag ttacggcgca ccgaaaattg gtgacattcc gcgtgatttc cgcgctgtgc
    65281 tggtgccgag cgacgataaa gtcggcccat tcggggcgaa atcgatctcg gaaatcggtg
    65341 taaatggcgc cgctccggcg attgctaccg caattcacga tgcctgcggc atctggttac
    65401 gcgaatggca tttcacaccg gagaaaatac tcaccgcgct ggaaaaaata taagtaataa
    65461 atggccgctt cggcggcctg tttctctgtt tgaacgatga ctaaatagtg actgcgaaaa
    65521 tgagataagc cgttctcaag gcgtcctgcg ccccaaattc gtgggaattt attttactaa
    65581 ttcagatgat caaatttact ttaaaaggag tgaagggatg tctgatataa accatgcagg
    65641 ttctgacctt atatttgaac tggaggatcg ccctcccttt catcaggctc tcgtaggtgc
    65701 cattacccat ctgttggcaa ttttcgttcc gatggtaacc cccgcgttaa tcgtgggtgc
    65761 ggccttacag ctttccgctg aaacaactgc ctatcttgtt tctatggcga tgatcgcctc
    65821 tggtattggt acctggttac aagtaaaccg ctacggcatc gtcggttctg gtttactttc
    65881 aattcagtca gtcaattttt catttgttac ggtcatgatt gcgctgggta gcagcatgaa
    65941 aagcgacggt tttcacgaag agttaatcat gtcgtcgctc ctcggcgtct ctttcgttgg
    66001 cgcatttctg gttgtcggct cttcttttat cctgccctat ttacgtcggg ttattacgcc
    66061 taccgtcagc ggcattgtgg tgctgatgat cggcttaagc ctgattaaag ttggcattat
    66121 tgattttggc ggtggttttg cagctaaaag cagcggtacg ttcggcaatt acgagcatct
    66181 cggcgttggc ttattggttt tgattgtggt gatcggcttt aactgctgcc gcagtccgtt
    66241 gctacgcatg ggtgggatcg ccattgggct atgtgtcggc tatatcgcat cgttatgcct
    66301 gggcatggtg gatttcagca gtatgcgcaa tttgccgtta atcaccatcc cgcatccgtt
    66361 caaatacggc tttagtttta gcttccatca gttcctggtg gttggcacga tttatctgct
    66421 tagcgtgctg gaagctgtcg gcgatatcac cgccacggca atggtttccc gccgtcccat
    66481 tcagggggaa gagtatcagt cccgactgaa aggcggcgtg ctggcagatg gtctggtttc
    66541 tgttatcgcc tccgctgtcg gttcattacc attaaccacg tttgcgcaaa ataatggggt
    66601 tattcagatg actggcgtcg cttcacgtta tgtcgggcga accatcgcgg taatgctggt
    66661 tatcctcggc ttatttccga tgattggcgg cttttttacg accattccct cggcagttct
    66721 gggaggcgca atgacgttga tgttttccat gattgccatc gcagggattc gcatcatcat
    66781 caccaacggt ttaaagcgcc gagaaacact tattgtcgcc acttctttag gtttaggact
    66841 tggcgtctcc tacgatcccg aaatttttaa aatattgcca gcctctattt atgtattagt
    66901 tgaaaaccct atttgtgctg gcgggttaac tgcgatttta ttaaatatta tcctccctgg
    66961 tggctaccga caggaaaacg ttctgcctgg tattacctca gcggaagaga tggattaaca
    67021 gtaaaggagt caatgatgtc aggagaacac acgttaaaag cggtacgagg cagttttatt
    67081 gatgtcaccc gtacggtcga taacccggaa gaaattgcct ctgcgctgcg gtttattgag
    67141 gatggtttat tactcattaa acagggaaaa gtggaatggt ttggcgaatg ggaagacgga
    67201 aagcatcaaa ttcctgacac cattcgcgtg cgcgactatc gcggcaaact gatagtaccg
    67261 ggctttatcg atacacatat ccattatccg caaagtgaaa tggtgggggc ctatggtgaa
    67321 caattgctgg agtggttgaa taaacacacc ttccctactg aacgtcgtta tgaggattta
    67381 gagtacgccc gcgagatgtc ggcgttcttc atcaagcagc ttttacgtaa cggaaccacc
    67441 acggcgctgg tgtttggcac tgttcatcca caatccgttg atgcgctgtt tgaagccgcc
    67501 agtcatatca atatgcgtat gattgccggt aaagtgatga tggaccgaaa cgcaccggat
    67561 tatctgctcg acactgccga aagcagctat caccaaagca aagaactgat tgaacgctgg
    67621 cacaaaaatg gtcgtctgtt gtatgcgatt acgccacgct tcgcccctac ctcatctcct
    67681 gaacagatgg cgatggcgca acgcctgaaa gaagaatatc cggatacatg ggtacatacc
    67741 catctctgtg aaaacaaaga tgaaattgcc tgggtgaaat cgctttatcc tgaccatgat
    67801 ggttatcttg atgtttacca tcagtacggc ctgaccggta aaaactgcgt ctttgctcac
    67861 tgcgtccatc tcgaagaaaa agagtgggat cgtctcagcg aaaccaaatc cagcattgct
    67921 ttctgtccga cctccaacct ttacctcggc agcggcttat tcaacttgaa aaaagcatgg
    67981 cagaagaaag tcaaagtggg catgggaaca gatatcggtg ccggaacaac ttttaacatg
    68041 ctgcaaacgc tgaacgaagc ctacaaggta ttgcaattac aaggctatcg cctctcggca
    68101 tatgaagcgt tttacctggc cacgctcggc ggagcgaaat ctctgggcct tgacgatttg
    68161 attggcaact ttttacctgg caaagaggct gatttcgtgg tgatggaacc cactgccact
    68221 ccgctacagc agctgcgcta tgacaactct gtttctttag tcgacaaatt gttcgtgatg
    68281 atgacgttgg gcgatgaccg ttcgatctac cgcacctacg ttgatggtcg tctggtgtac
    68341 gaacgcaact aataataaaa ctttaacatc ctcgtgagga catcattatg tctggagaca
    68401 tcctacaaac accggacgca ccaaagccac agggcgcgct ggataattat tttaaaatta
    68461 ccgctcgtgg cagtaccgtt cgtcaggaag tactggctgg cttaacgacc tttctggcca
    68521 tggtttattc cgttatcgtc gttccgggaa tgctgggcaa agcaggtttt cctcccgcag
    68581 ctgtgtttgt tgccacctgt ctggtcgcgg gcttcggctc gttgctgatg gggttatggg
    68641 ccaatttgcc aatggcgatt ggttgcgcta tttccttgac ggcgtttacc gcattcagtc
    68701 tggtactcgg gcaacaaatt agcgttcctg tcgcactggg cgcagtcttt ctgatgggcg
    68761 tcatcttcac cgctatttcc gtaaccggtg tgcgtacctg gatcttacgt aatttgccga
    68821 tggggatcgc tcacggtaca ggtatcggta tcggcctgtt tctgctgctg attgctgcta
    68881 acggtgtggg aatggttatc aaaaacccga ttgaaggctt gccagtggcg ctcggtgcct
    68941 ttacctcctt cccggtgatg atgagcttac tggggctggc ggtcatcttc ggtctggaaa
    69001 agtgccgcgt acccggcggg atcttgttgg tgattattgc aatttcgatc atcggcttaa
    69061 tctttgaccc agcggtgaag taccacggtc tggtggcaat gccaagcctg actggcgaag
    69121 atggtaagtc tctgattttc agcctcgata ttatgggcgc actccagcca actgtacttc
    69181 cgagtgtact ggcattggtg atgaccgcag tgttcgacgc cactggcacc atccgtgccg
    69241 tcgccggtca ggcgaatttg ttggataaag acaaccagat catcaacggc ggcaaagccc
    69301 tgaccagtga ctcagtaagt tcaatattct ccggcctggt gggcgcagcg cccgcggcgg
    69361 tttatatcga atcagcggca ggaaccgccg ccggggggaa aaccggttta accgcaaccg
    69421 tggttggtgt gttgttcctg atgattctgt ttttatcacc gttatcgttt ctgatccccg
    69481 gttatgccac agctcccgca ctgatgtacg taggtttgct gatgttaagc aacgtctcga
    69541 agctggattt caacgatttt attgacgcaa tggctggcct ggtgtgtgcc gtgttcatcg
    69601 ttctgacttg taatatcgtt accggtatta tgctgggctt tgtaacactg gtggtgggtc
    69661 gcgtctttgc gcgcgaatgg caaaagctga atattggtac ggtgatcatt actgccgcac
    69721 tggtcgcgtt ttacgcgggt ggttgggcta tctaatagat tctccgcgcc tttcaacgcg
    69781 gagtttttta tcaaagagga ttcgcgccgc tggcagcaat acgctgctgg cggatacgcc
    69841 tgagttctct ttcagtcagc aactgcaacg cctgtgtcgg gcaagattca acgcaggctg
    69901 gcccctcttc ccgctgttca cagagatcgc atttcacgat ttgttgccgg gtatcacccg
    69961 gcaatgactg aatggtgatc atcccaaacg ggcacgcgct aacgcaactc tgacagccaa
    70021 tacaacgggc agaattggcc tgcaccacct gctcgcccat cgtcagcgcc cccacggggc
    70081 aagcgccaac acaaggggcg ttttcacact gatggcacat caccggcgca ctgatgctat
    70141 ccagccgttg taccttcagc cggggtaaaa agatatcggc attcaactcc tgttctgaag
    70201 ggtgagcgac cacacaggcc acttcacaag tacggcagcc aatacaggca gccggattaa
    70261 cgataattaa cgatttcatc acgatgcctt cgtatcaaac agagttaaca tatcgcgcgc
    70321 cgcctgcctt cctgcggcca ttgcagtgac aaccagatcc gcgccatgaa ctgcatcacc
    70381 accagcaaag acttttttca gatgcgtctg ggtaggtaaa tacccgacgt cgccggtttg
    70441 aatcaggccc catttatcga gtttaattcc gctgccctgc aaccacggca tggtatgtgc
    70501 ctggaaacca aaggccataa tgagaacatc ggcgggcaat tcaaactctg aacccgcaac
    70561 cggacgagga cgacggcgac catccggccc cggctcaccc atagcggtac gaatcaggcc
    70621 caccgccgtt aagcgtccat cttcatcaca agcgatatat tgcggctgaa cattgaactg
    70681 aaactcaaca ccttcctcgc gggcattgac cacctctttg cgcgagcccg gcatactgac
    70741 ttcatcacga cgatacgcac aggtcacgct ggcggcatta aggcggatgg aagtccgcaa
    70801 acaatccatt gtcgtatcgc caccgcccaa taccacaact cgcttacctt ccacatccgt
    70861 cagcggatac tctgcagact ccggcaatcc catgagctgg cgggtatggg cggtcaggaa
    70921 cggtaacgcc tgaatgacac caggcgcatc ttcatgcggc agatctgctc gcatcatccc
    70981 gtaagtcccc acgccgagga aaacagcatc atattccgcc gttaattcgt tgaaagagat
    71041 atcgcggcca atttcacagt taagatgaaa gtcgattccc attgcggtga atatctctcg
    71101 ccgctggctt aataccgttt tatcgagttt gaaaggagga atgccaaaag tcagcatacc
    71161 gccaatttct ggatggcgat caaagacatc aacctgaact cccgcgcgcg ccagaatatc
    71221 agcacaccct aatcctgcag gtccagcgcc aatcaccgcc actttttcga tacggggaac
    71281 aactttgctg acatcgggac gccagcccat cgccagcgca gtatcggtga tgtagcgttc
    71341 cagattaccg atagagactg cgccagagtg atctttcaaa gtacatgcgc cttcacaaag
    71401 acggtcctgt ggacataccc tgccgcagat ttcgggtaag gaactggtct ggtggcaaag
    71461 ttctgccgct tcaataatct ttccttcctg taccagacgg atgtaatccg gaatagcgtt
    71521 atgcagcggg caatgccagt tgcagttagc tttttcggca caataaacac agcggtcact
    71581 ctcataagtc gcttgttgtg gatccagccc gcaatagatt tcgccaaagt gggttttccg
    71641 ttcactcgct gaaattttat ctgcgccttt acgcgagtta acggggagca acgctgcact
    71701 gcgagatagc tgagcgtctg atgacgcttt tcctgctgcc gttttgcgct ggcgggccat
    71761 ctttatctgc tgcaaccctt tatcgtccat cagccgtaac gcctgtgttg ggcagacgtc
    71821 aatacaggct tgcgtgccgg aactgcgctg gttacaaagg tcgcatttct gtgcaatcgt
    71881 atcgaccatc tcaacgacgc caaaggggca agcgattgcg catcttttac aaccaataca
    71941 tttttgctcg tccagttgta cgctatcgga ctggaaagtc agagcattaa ccggacaagc
    72001 cgtaacgcaa ggggcattgt tgcagtgatg gcaggccacc ggattcgcag cctggccctt
    72061 ccctacaacg tggatacgcg gtcgaaagtc actgtgactc agcggccagt tttcttgatt
    72121 atgtgccacc gcacaggcaa tttcacaagc atggcagcct atacattccg cagcttcagc
    72181 agcgataaac ttattcattt gcatcccttt catttaatga gttatgtctt ctcaaatttc
    72241 gcgatgcaat aaacgggcat atattaggta gcatgaccgt tttaattata attttgcaac
    72301 tgagttcaaa ttattgcccc tcaatctatg ccgctttcat tatttcaaca ttgccgatca
    72361 aaagaatgct atccagataa aaacgattaa atgcgagagt gcgacatgcc agaatgatta
    72421 atgaaatatc aatatgagaa aattacagat aattccattg tcaggttctc ttctcataat
    72481 cgtggtttaa ccacgattat gtgattgcct ccgcaattcc tgtctctaac tccccttcct
    72541 cgcaaaaact ggcactccac gagcatgtgt ttagacagtt tcatgaacat aaacggttgc
    72601 tttttactct ggcgggcgaa aggagaaaca ctgatgagcg ccatagattc ccaacttccc
    72661 tcatcttctg ggcaagaccg cccaactgat gaggttgacc gcatattatc acctggaaag
    72721 ctgatcatac tcggtctgca acacgtcctt gtcatgtacg caggtgcagt cgctgttcct
    72781 cttatgattg gtgaccgact cggcctatca aaagaagcta ttgcgatgct catcagctcg
    72841 gatctctttt gctgcgggat cgtcacatta ttgcaatgta tcggtatcgg ccgctttatg
    72901 gggatccgcc tgccggtgat tatgtcggtg accttcgccg ctgtaacacc aatgatagct
    72961 attggtatga acccggatat cggcctgctg gggatattcg gtgccactat cgccgcgggt
    73021 tttatcacca cattattagc gccacttatc ggtcgcttga tgcctttatt cccgccactg
    73081 gttaccggtg tggttattac ttctatcggg cttagcatca ttcaggtggg tattgactgg
    73141 gccgccggag gtaaagggaa tccgcaatat ggtaatcccg tttatttagg tatctccttt
    73201 gccgtcttaa tttttatctt gctcattact cgctatgcga aaggatttat gtccaacgtc
    73261 gccgtattac tggggattgt atttggcttt ttactttcgt ggatgatgaa tgaagtcaat
    73321 ttatccgggc tacatgatgc ttcatggttt gcgattgtca cgccgatgtc atttggtatg
    73381 ccgattttcg atcccgtttc cattctgacc atgactgccg tgttaatcat cgtgtttatc
    73441 gagtcgatgg ggatgttcct ggcactgggt gaaatagtcg gtcgtaaact ctcttcacac
    73501 gatattattc gcgggctgcg tgtcgatggt gtagggacaa tgataggcgg aacgtttaac
    73561 agcttccccc acacgtcatt ttctcaaaac gttggcctgg ttagcgtgac gcgcgttcat
    73621 agccgctggg tgtgcattgc ttccggtatc attttgatcc tgttcggcat ggtgcccaaa
    73681 atggcggtgc tggtggcctc gattccgcaa tttgtgctgg gcggcgcggg gctggtgatg
    73741 ttcggcatgg tactggcgac agggattcga attctgtcgc gctgtaacta caccactaac
    73801 cgttacaacc tctatattgt ggcgatcagt ctcggtgttg gcatgacgcc aacgctctct
    73861 cacgatttct tttctaagtt accggccgta ctgcaaccgt tgctgcatag cggcattatg
    73921 ctcgcaaccc ttagcgccgt tgtgctgaac gtcttcttta atggctacca gcatcatgct
    73981 gacctggtga aggaatccgt ctctgataaa gatttaaaag tcaggacagt acgtatgtgg
    74041 cttctgatgc gcaagctgaa gaaaaatgag catggagaat aatatgaatc ttttaatgcg
    74101 cgctatattc agtctgctgt tgctttttac tctctctatt cctgtcattt ctgattgtgt
    74161 tgcaatggcc attgaaagtc gcttcaaata tatgatgctg cttttttaaa tggtttttac
    74221 ctgtcgacat ccgctcaaaa cgggcggttg tcgataaacg ctcacttggt taatcatttc
    74281 actcttctat tatctataat gatgagtgat cagaattaca tgtgagatat tatgcaaacg
    74341 gaacacgtca ttttattgaa tgcacaggga gttcccacgg gtacgctgga aaagtatgcc
    74401 gcacacacgg cagacaccct cttacatctc gcgttttcca gttggctgtt taatgccaaa
    74461 gggcaattat tagttacccg ccgcgccctt agcaaaaaag catggcctgg cgtgtggact
    74521 aactcggttt gtgggcaccc acaaccggga gaaagcaacg aagacgcggt gatccgccgt
    74581 tgccgttatg agcttggcgt ggaaattacg cctcctgaat ctatctatcc tgactttcgc
    74641 tatcgcgcca ccgatccgaa tggcattgtg gaaaatgaag tgtgtccggt atttgccgca
    74701 cgcaccacca gtgcgttaca gatcaacgat gatgaagtga tggattatca atggtgtgat
    74761 ttagcagatg tattacgcgg tattgatgcc acgccgtggg cgttcagtcc gtggatggtg
    74821 atgcaggcag ccaatagtga agcaagaaaa ttgttgtctg ctttcgcgca gcacaattaa
    74881 aaaaccccga catttgccgg ggttatgagc ataacgtaat gcttatttta ccggacgcat
    74941 cgccgggaac agaataacgt cgcggatggt atggctgttg gtgaacagca ttaccatacg
    75001 gtcgatacca attcccagac ctgctgtcgg cggtaagcca tgttccagtg cggtgacgta
    75061 atcttcatcg tagaacatcg cttcgtcgtc gcctgcatct ttcgcggcaa cctgatccag
    75121 gaagcgctgc gcctgatctt ccgcgtcatt cagttcgcta aagccgttac cgatttcacg
    75181 accaccaatg aagaactcaa agcggtctgt gatttccggg ttaacgtcgt tacgacgcgc
    75241 cagcggagaa acttctgccg gatattcagt aatgaaggtc ggctgaatca gatgtgcttc
    75301 tgccacttct tcgaagatct cggtaacgat acggcccaga ccccagctct tctcaacgtg
    75361 gataccgata gattcagcaa tcgctttcgc agagtcgaag ttgtccagat ccgccatgtc
    75421 ggtttccggg cgatatttct tgatcgcttc acgcatggtc agtttttcga acggtttacc
    75481 gaagtccagc gtcacgtcgc cgtaggtcac ttccgtctta ccgagaatat cctgtgccag
    75541 agtacggaac agcgattcgg tcagctcgat cagatctttg taatctgcgt aagccatata
    75601 gagttccatc atggtgaact ctgggttatg acgtacggaa ataccttcgt tacggaagtt
    75661 acggttgatt tcgaatacac gctcgaagcc accgaccacc agacgcttga ggtacagttc
    75721 cggcgcgata cgcaggtaca tgtcgagatc cagcgcgtta tggtgagtga taaacggacg
    75781 cgcagcggca ccgccaggga tcacctgcat catcggcgtt tcaacttcca taaagccgcg
    75841 gttcaccatg aactggcgaa taccagagag gatctgcgag cgcactttaa aggtgttgcg
    75901 ggattcatcg ttagagatga gatccagata acgctgacga tagcgcgctt cctggtcctg
    75961 caagccgtgg aatttatccg gcagcggacg cagtgctttg gtcagcagac gcagctcggt
    76021 acagtggata gacagttcgc cagttttggt tttgaacagt ttacctttcg cgccgaggat
    76081 atcgccgagg tcccattttt tgaactgctc gttgtaaacg ccttccggca gatcgtcacg
    76141 ggcaacgtac agctgaatgc ggccacccac atcctgcagg gtaacgaaag acgctttacc
    76201 cataatacga cgggtcatca tgcggccagc aacggcgact tcgatgttca gcgcttccag
    76261 ttcttcgttc tctttaccgt cgaattctgc gtgcaattgg tcagaggtat gatcgcgacg
    76321 gaaatcgttc gggaaggcaa tcccctgctc acgcaggttc gccagcttct cacgacgcgt
    76381 tttcagttca ttgttaagat cgactaccgc gtcagcgccc tgtgcgtgtt gttcagacat
    76441 gttggttcct cataaccctg ctttcaaact tgcttcgata aattgatcca ggctgccgtc
    76501 cagcacggcc tgcgtgttgc gggtttctac cccggtacgc agatctttaa tgcgggagtc
    76561 atcaaggaca taagaacgaa tctggctgcc ccagccgatg tcggatttgt tatcttccat
    76621 cgcctgtttc tcggcatttt tcttctgcat ctccagttca taaagcttcg ctttcatctg
    76681 cttcatggcc tggtctttgt tcttgtgctg ggaacggtcg ttctggcatt gggtcacgat
    76741 cccggttggg atgtgagtaa tacgcaccgc agattcggta cggttaacgt gctgaccgcc
    76801 cgcgccggac gcgcgataaa cgtcgatgcg cagatctgcc ggattgattt cgatatcgat
    76861 atcatcatca acttccgggt aaacaaacgc ggagctgaac gacgtgtggc gacgaccgcc
    76921 ggagtcaaac gggctcttac gcaccaggcg gtgaacgccg gtttctgtac gcagccagcc
    76981 ataagcgtaa tcgccggaga ttttgatcgt tacggattta atacccgcca cttcaccttc
    77041 cgactcttcg atgatttcag ttttgaaacc acgcgattct gcccagcgca gatacatacg
    77101 ctcaagcatg ctcgcccagt cctgtgcttc cgtaccgcca gaccccgcct gaatatcgag
    77161 gtagcagtcg gcgctgtcat attcgccaga gaacatacgg cggaactcaa gctgcgccag
    77221 tttttcttcc agggcgtcga gttcagcaac ggcttcgtta aaggtttctt cgtcgtcagc
    77281 ttctacagcc agttccagca gaccagaaac atcttccagc ccctgtttca tttggtcgag
    77341 ggtgtcgaca acggcttcga gggaggaacg ctctttaccc agcgcctgtg cgcgttcggg
    77401 ttcgttccag acatccggct gttccagctc ggcgtttact tcttccagac gctctttctt
    77461 ggcgtcgtag tcaaagatac cccctaagaa cgtcggagcg ttccgtgagg tcctgaatgc
    77521 gattatttac cggattaatt tcaaacatgg tctgatttct tttattgagc tagtcaaaat
    77581 gcggtgataa gagcgggatt gtacccaatc cacgctcttt tttatagaga agatgacgct
    77641 aaattggcca gatattgtcg atgataattt gcaggctgcg gttgccgcga aactcgttga
    77701 tatcgagctt gtaagccagt tgcacttcgc gcacgccgtt atccggccag agggcggtat
    77761 cgacattaaa agcaataccg tccagcagcg gaccgccgcc gaccggttcg accatgactt
    77821 tcaaatgacg ttcgcccacc agccgctgtt gcagcagacg aaaatgacca tcaaacagcg
    77881 gctccgggaa catctgcccc cacgggccag catcgcgcaa cagctgcgcc acttccatgg
    77941 tcatttcggc cggacttaac ggaccgtccg acaccacttc gccttgcaat agcgaagggt
    78001 ccagccactc agtaaccagt tcgccaaacc gttgttgaaa gagttcgaat ttctcctctt
    78061 ccagcgacaa acccgccgcc atcgcatgac cgccaaactt gagcatcatg ccggggtaga
    78121 gtgtgtctaa tcgctccagc gcatcacgca tatgcagccc ctgaatggag cgaccggaac
    78181 ctttcagcgt accatcacca gctggcgcaa aggcgataac cggacggtga aaacgctctt
    78241 tgatacgcga tgccagaatg ccaacaacgc cctgatgcca ttcggggtga tacattgcca
    78301 gcccacctgg cagcgtgtcg cggctacgtt ccagcttctc gcacagagtg agcgcttcaa
    78361 cctgcattcc ctgttcgatc tctttacgtg tttggtttag tgcatcgagt tcattcgcca
    78421 gcacacgcgc ttcgccaatg ttgtcacaca acaacagcgc cacgccaacg gacatatcgt
    78481 ccagtcgccc ggcagcattg agacgtggcc ccagcgcaaa acctaaatcg ctggcggcga
    78541 gtttttgtgg atcgcgattg gccacttcca gcagcgcttt aatccccgga cggcactttc
    78601 cggcacggat gcgactcatc ccctgccagg tcagaatgcg attattagcg tccagcggca
    78661 cgacgtccgc cactgtcccc aacgctacca gatccagcag ttctgccaga ttaggaattg
    78721 cgatgccgcg ctcatcaaac cagccctgat cgcgcaaaaa ggtgcgcagc gccagcatca
    78781 gataaaacgc cacacccacg cctgccagtg atttcgacgg gaaattacag tcgcgcaagt
    78841 tagggttaat gatcgcttcc gctgcgggta atgtgtcgcc tggcaaatgg tgatcggtaa
    78901 caataaccgg aatgcccaac gaacgagcgt gttcaacccc cgcatgggag gaaataccgt
    78961 tatccaccgt gacaattaac tgcgcgccac gggcatgagc ctgatcgact acttccgggc
    79021 ttaagccgta accgtcttcg aaacggtttg gaaccaggta gtcgatattg ctgcaaccaa
    79081 gcgagcgcat cgccagcacg cttagagccg tgctggtggc accgtcggcg tcgaaatcac
    79141 cgaccacaat aatccgcgtg ccttcgcgaa aagcgttgca aaggatctca acggcctttt
    79201 cgacgccgct cagttgctgc cagggcagca tgcctttaac actgcgttcc agttcctgcg
    79261 cgctacgcac accccggctg gcatataaac ggcgcagcaa gggaggcaat tcagcgggca
    79321 agtcggccgt ttcatcgact tcacggcgac gaagttgtat ctgttgtttc acgcgaatta
    79381 tttaccgctg gtcatttttt ggtgttcgtc gaggaattct ttcatgtctt tcggcggctg
    79441 gtaacccgga acaagtgtgc cattgctcag cacaactgcc ggagtaccgc taacgccaag
    79501 ctggacgcca agtgcgtaat ggtcggcaat atccacgtcg cagctggctg gtgcgacgct
    79561 tttacctgcc atcacatcat caaacgcttt gtttttatct ttcgcacacc agatagcttt
    79621 catttctttc tctgcatcgc tgtccagccc ctggcgcggg aaagcaagat aacgcacggt
    79681 gatccccagc gcgttatagt ctgccatttg ctcatgcagt ttgtggcagt aaccacaggt
    79741 aatatcagta aacacggtga tgacgtgttt ttcctgcggc gctttataaa cgatcatctc
    79801 tttttcaagc gcattcaact gctttaacag catcttattg gtgacattga ccggagccgt
    79861 gccactaacg tcatacattg gcccctgaat gatatgttta ccatcatcgg tgatgtacaa
    79921 cacaccgctg ttagtcagaa ctgtcttcat gccagctacg ggcgcgggct gaatatcgct
    79981 gcttttgatg cccattttgg ctaacgtttg ttgaattgcc gcgtcatcag cctgaacaaa
    80041 gcctgaaaac gctgccaaca aggtaaataa cataaaacct ttcttcataa attttcctgt
    80101 tcttttcaga catcacgccc gcgggtgatg ctgttgatga agttgccgca gacgctcggt
    80161 agcgacatgc gtataaattt gcgtggtgga gagatcgctg tggcccagta gcatctgcac
    80221 cacgcgtaaa tccgcaccat gatttaataa gtgagtggca aaagcgtgac gcaacacatg
    80281 cggtgacagc ttttcgctgt cgatacccgc cagcacggca taatgtttaa tgcggtgcca
    80341 gaaggtctgt cgcgtcatct gctgcgcacg ctggctggga aacaacacgt caattgacac
    80401 accattcaac agccacggac gcccatgttc cagataggtt tccagccagt aaaccgcctc
    80461 ttcacctaac ggcaccaggc gctctttgtt gcctttacca atgacccgca ctacgccctg
    80521 acgcaggctg atatcactca ttgtcagccc gaccagttca gagacacgca gtccggtagc
    80581 atacaacact tcaagcatgg ctttatcgcg tagctccagt ggctgatcaa ttaatggtgc
    80641 ctgtaataaa cgttcgacct gcgcttcgct taaatctttt ggcaaacgct ggggcaattt
    80701 cggtgaagcg agatgcgcac tgggatcatc ttcacgaaac ttttcgcgat aaagatactg
    80761 gaacaatcgg cgcactgcac tcagcaaacg cgctgagctg gtggctttat acccgccctc
    80821 cagccgttct gccagtaatg cctgcaaatc gtcactttgc gccgtcgcca gcgtcaaccc
    80881 gcggtgatgc aaccactcca ccatcattga cagatcgcga cggtaagcgt tcaacgtatt
    80941 ttcagccaga tttttttcca gccacagagc atcaagaaac tgctcgatgc gtgccagatc
    81001 ctgtttcact tgcgcccctt atggtcactc atttgatcca ttatgcctta ttgtgccgtg
    81061 actaaagcga ttctgataca ctagccgcaa aagccacagc agaatcgaga agcttacgtt
    81121 atgaatatgg gtctttttta cggttccagc acctgttaca ccgaaatggc ggcagaaaaa
    81181 atccgcgata ttatcggccc agaactggtg accttacata acctcaagga cgactccccg
    81241 aaattaatgg agcagtacga tgtgctcatt ctgggtatcc cgacctggga ttttggcgaa
    81301 atccaggaag actgggaagc cgtctgggat cagctcgacg acctgaacct cgaaggtaaa
    81361 attgttgcgc tgtatgggct tggcgatcaa ctgggatacg gcgagtggtt cctcgatgcg
    81421 ctcggtatgc tgcatgacaa actctcgacc aaaggcgtga agttcgtcgg ctactggcca
    81481 acggaaggat atgaatttac cagcccgaaa ccggtgattg ctgacgggca actgttcgtg
    81541 ggtctggcgc tggatgaaac taaccagtat gaccttagcg acgagcgtat tcaaagctgg
    81601 tgcgagcaaa tcctcaacga aatggcagag cattacgcct gatgtcactg acggcttagc
    81661 acatatgctt tgccgtcatc tttatctttg cgtctcttgt tgtaacaata tccgccgtaa
    81721 atcccgccat tcggcttcgt ccatgctgtc agctgccagc cataaatgtt gccgttttcc
    81781 gctgtcagaa cgtaaacgca gcatcatgcc gctcttaatc atccacggcg ctttgacgat
    81841 actccactcc tgcccttgcc aacgcaaacg cccgtccatc aacaagcgaa tttccccctg
    81901 gcgagcatta atgcgccgct ggctgcgaac gcaatcaaac accaccagcg aaagtaacac
    81961 catccataac ggggtgtaac tgagcggcca gggcatgagt aaaataacag cggcaaccag
    82021 cccatgaatc agcaaggaaa gccactgtgc gcgccaggag acgcgcaaat cagattgcca
    82081 caggaccacg ttcccggttc cgtgtctgga tgagtcggac catcatttcc agttctgcat
    82141 cggctggttt accgtgattc atcagccagt taaacaggtc cggatcgtca cattccagca
    82201 gacgaataaa gatgcgtttt tcgtcatcgc ttaagctgtc gtactcatgt tcgaaaaacg
    82261 gcatgattga aatatcgagt tcgcgcatac cacggcggca tgcccaatga atgcgggctt
    82321 tgttgttaat gtccatcttc ttcctgtctc acgaaaatcc agtacccggc tattgtaacg
    82381 tgtttttcga cttcttttac gggaatatca gtaaacacaa tcgcgatcgc gaaattaatt
    82441 cacaacaatt caagggcttc atttttttgg aagtcgcctc gcagaaggca cagatcgcgt
    82501 agtgaaagca cttgcattgc ctcatagctc ttttaccatt agtgattaat acgccgttaa
    82561 gcaactcagg actctattat ggcttttaca ccttttcctc cccgtcagcc tacggcttct
    82621 gcccgtttgc cactgacgct gatgacgctt gatgactggg cgcttgccac tattactggc
    82681 gcggacagcg aaaaatatat gcaaggccag gtgacagcag atgtcagcca gatgacagaa
    82741 gatcagcacc tgctcgccgc ccattgcgac gccaaaggta aaatgtggag caatttacgt
    82801 ctgttccgcg acggcgatgg ctttgcatgg attgaacggc gcagcgtgcg tgaaccacag
    82861 ctgactgaac tgaaaaaata tgcggtgttc tctaaagtga ccatcgcgcc agacgacgag
    82921 cgtgtgctgc ttggtgttgc cggttttcag gcgcgcgccg cgttggcaaa tctctttagt
    82981 gaactacctt cgagagaaaa acaggtagtc aaagaaggcg cgaccacgtt gctatggttt
    83041 gaacacccgg cagaacgttt cctgatcgta accgatgaag ctactgccaa tatgctgacc
    83101 gataaactgc gcggtgaagc ggaactgaac aatagccaac agtggctggc attaaacatt
    83161 gaagcgggtt ttccggtgat tgatgccgcc aacagcgggc agtttatccc ccaggcgacc
    83221 aacctccagg cgctgggcgg tatcagcttt aagaaaggct gctataccgg acaagagatg
    83281 gtggcgcgag caaaattccg tggtgccaac aaacgcgcgc tctggttgct gaagggtagc
    83341 gccagccgac tgccggaagc tggtgaagac ttagagctga aaatgggcga gaactggcgt
    83401 cgtaccggta cggtgctggc tgcggtcaaa ctggaagatg gtcaggttgt ggtgcaggtt
    83461 gtcatgaata acgatatgga accggacagc atcttccgca tgcgtgacga tgcgaataca
    83521 ttgcgtatcg agccactgcc atattcgctg gaagagtaaa tctctgtatc gcatcaggca
    83581 tttatcgcct gatgcgacac tggtgtgtct tttcaggcct acaaaggcac gccaattacg
    83641 cctgcccaat atacaaatag atcgccagaa agtggcacac actaccgccg agcacaaagc
    83701 cgtgccagat ggcatgattg tatggaatgc gcttgcagac gtagaaaatc accccgagcg
    83761 aataaaccac accgcctacc gccagtaagg taacgctgcc cgccgcgagc ttaactgcca
    83821 tttcataaat taccaccagc gacagccagc ccatcgccag ataggtcacc agagataaaa
    83881 ttttgaatcg gtgcgcgatg gtcagtttga acagaatccc cagcaatgcc aggctccaga
    83941 taacaatcat caacccgcgc gctaacggag aatccagccc caccagcaaa aacggtgtgt
    84001 aggttccggc aatcaataga taaatggcac agtggtcaaa tttcttcagc cacatttttg
    84061 cccgttgatg gggaatggcg tgatagagcg tcgaagcgag gaacagcagg atcatactgc
    84121 cgccatagag gctgtaactg gttatcgccg tggcgctggc attgagatcc accgcctgaa
    84181 ccagtagcaa caccagccca acgataccaa acaccagccc aatgccgtga ctgacgctgt
    84241 tggcaatttc ctctgccagc gaatatccct gcttaatgag gggcttctga accataactt
    84301 actccggaaa aacgtacacg cacatgtata cctctccagc gtaactgaga atagttccag
    84361 tgaacacctg ttagctaaaa taaattctga tttgagatta atctccgaaa aatcatgaaa
    84421 ttaaatgcga aatttcaact aacaggcgtg agttcaactt aaagacattt aaattcaatc
    84481 acataaaact gtgtctggtc gggatagatg tcggcaatga cctttttcag ttcagtcagg
    84541 ggcatatttt cctgctctgc atgtttttcc gtcagcgtat ccagcgttac ggttgaggtt
    84601 gcggtgactt caatcgtgca aaaataaccg tcatcttcaa aacgtccgac acgaagcaca
    84661 tcacccgttt tgaagtgcga ttcagactcg tcgcggatgg tgatggtttt acgtccagcc
    84721 agaatgtcat cctggaaacg ttgaaaaaaa gtgatgtcgt ttggctgcat ggtaatattt
    84781 cctgtaagaa ttgactcatc tggagcctat gatagtgaaa aaactcacct taccgaaaga
    84841 tttcttatgg ggcggcgcag ttgccgctca tcaggtcgaa ggcggctgga acaaaggcgg
    84901 caaagggccg agcatttgtg acgttctgac cggtggcgca cacggcgtgc cgcgcgaaat
    84961 caccaaagaa gttgtgcctg gcaaatacta tccaaaccat gaagccgttg atttttatgg
    85021 tcactacaag gaggacatca agctatttgc cgaaatgggc ttcaaatgtt ttcgtacttc
    85081 catcgcctgg acgcgcattt ttccaaaagg cgatgaagct cagccaaacg aagaagggct
    85141 gaagttctac gatgatatga tcgatgaact gctgaaatac aacatcgaac cggtgatcac
    85201 cctctcccac tttgaaatgc cgctgcatct ggtgcagcaa tacggtagct ggaccaaccg
    85261 taaagtggtt gatttctttg tccgtttcgc ggaagtggta ttcgaacgct ataagcacaa
    85321 agtcaaatac tggatgacct tcaacgaaat taacaaccag cgtaactggc gtgcaccgct
    85381 gttcggttac tgctgctccg gcgtggtgta taccgaacat gaaaacccgg aagagaccat
    85441 gtatcaggtg ctgcatcacc agtttgtcgc cagcgccctg gcggtgaaag ccgcgcatcg
    85501 catcaacccg gagatgaaag tcggttgtat gctggcgatg gtgccgctct atccctacgc
    85561 ctgtaacccg gacgatgtga tgtttgccca ggagtcgatg cgcgaacgtt acgtctttac
    85621 cgatgtgcag ttgcgtggct attacccgtc ctatgtgttg aacgagtggg aacgccgcgg
    85681 atttaacatc aaaatggaag acggcgatct ggatgtactg cgcgaaggca cctgcgatta
    85741 tctcggcttc agctattaca tgaccaacgc ggtgaaggcc gaaggtggca ccggcgatgc
    85801 gatttctggt ttcgaaggca gcgtaccgaa tccgtatgtt aaagcgtctg actggggctg
    85861 gcagattgat ccggttggtc tgcgttacgc gctttgcgag ctgtatgagc gttaccagaa
    85921 gccgctgttt attgtcgaaa acggttttgg cgcttacgac aaagtggaag atgatggcag
    85981 catcaatgac gattaccgca ttgactacct gcgcgcccat attgaagaga tgaaaaaagc
    86041 ggtgacttac gatggcgtgg atctgatggg ctacacaccg tggggctgca tcgactgcgt
    86101 gtcgttcact accgggcagt acagcaaacg ctacggcttt atctatgtga ataaacatga
    86161 cgacggtact ggcgatatgt cgcgttcacg taagaagagc tttgactggt acaaagaggt
    86221 gattgccagc aacggcgaga atctgtaatt agccgatgtg cctgatgtgc tccgctcatc
    86281 aggcctggca ctcttataat atattgagct cgcaatattt tatattacac ctcagccgaa
    86341 acacacaaag cgcactttat taacaagcga aaaaggcgcc gaagcgcctt tataagatag
    86401 tcgaatcagt aaattactgg tattcgctaa tcggtacgca ggagcagaac aggttacggt
    86461 cgccgtaaac atcatccaga cgtttcacgg tcggccagta tttgtctgcc acacctgccg
    86521 ggaatactgc aacttcacgg ctgtacggat gcgcccactc ggcgaccagt tcgctttgaa
    86581 tgtgcggcgc attcaccagc gggttatctt ccagcggcca gacaccggct ttcacctgat
    86641 caatttctgc gcggatagcc agcatcgcat cgataaagcg atccagttcc actttgcttt
    86701 cagattcagt cggttcaacc atcagcgtac ccgccaccgg gaacgacatg gtcggcgcgt
    86761 ggaaaccgta gtcgatcagg cgcttggcaa tatccagctc gctgatgccg gtttcttctt
    86821 tcagcgggcg aatatcgaga atacattcgt gcgccacgcg accgtcgcga ccggtataca
    86881 gcaccgggaa ggcatcctgc agacggctgg caatatagtt ggcgttaaga attgccacct
    86941 ggcttgcttt tttcagccct tctgcgccca tcatgcggat gtacatccag ctgattggca
    87001 ggatagaggc actaccgaac ggtgccgcag aaaccgcgcc ctgacgggtt aacatgcctt
    87061 cgatttgcac cacgctatga cccggtacaa acggagccag atgcgctttc acaccgatcg
    87121 gtcccatacc cggaccacca ccgccatgcg gaatgcagaa agttttatgc aggttaaggt
    87181 gggaaacgtc cgcaccaata aaacccggag aggtgatgcc aacctgagcg ttcatgttcg
    87241 cgccatcaag gtaaacctga ccgccgaact gatgcacgac ttcacacact tcacggatcg
    87301 tttcttcata cacgccgtgg gtagaaggat aggtcaccat gatacaggag aggttatcgc
    87361 ccgcctgttc cgctttcgcg cgcagatcag tcagatcgat gttgccgttt ttatcacacg
    87421 ccacaaccac cacctgcatt cctgccatat gcgcggaagc cgggttagtg ccgtgcgcag
    87481 aagccgggat caggcagata tcgcgatgcc cttcgttgcg gctttcatga taatgacgaa
    87541 tcgccagcag gcccgcgtat tcgccctgtg cgccagagtt cggctgcata caaacggcgt
    87601 cgtaaccggt cagtttcacc agccagtcag ccagctgcgc gatcatctgc tgataacctt
    87661 cagcctgttc cggcgggcag aacgggtgca gttcggcaaa ttccggccag gtgatcggga
    87721 tcatctcggc ggcggcgttc agtttcatgg tgcaggaacc cagcgggatc atcgcctgat
    87781 tcagcgccag atctttacgc tccagcgagt gcatatagcg catcatttcg gtttcgctgt
    87841 ggtagcgatt aaacaccgga tgggtgagga tttcgtcgtc gcgcagcatc gcagcctgaa
    87901 tagagcgact gtcgtgagcc acgtctttgt ccagtgtgtc gatgtccagc ccgtggttat
    87961 cgcccaacag cacgctgaaa agctgcatca cgttttcgcg cgtggttgtt tcatcaaggg
    88021 tgatcccaac cgcgttcaga atatcgctac gcaggttgat ttcagccgct tcggcacgcg
    88081 ccagcacgcc cgctttgtcg gccacttcca cacacaaagt atcgaaatag tgcgcatggc
    88141 gcagcttcag acctttttgt tgcaggcccg ccgccaggat atcggtcaga cggtggatgc
    88201 gattagcgat acgtttcagg ccaaccggac cgtgataaac ggcatacagg ctggcaatgt
    88261 ttgccagcag tacctgggaa gtacaaatgt tggagttcgc tttctcacga cgaatatgtt
    88321 gctcgcgagt ctgcatcgcc atgcgcagag cggtgttgcc agctgcatct ttcgatacac
    88381 cgataatacg gcccggcatt gagcgtttgt attcgtcttt cgccgcaaag aatgccgcgt
    88441 gtgggccacc gtagcccatc ggtacgccaa agcgttgcgc cgaaccaaaa acaatatccg
    88501 cgccctgttt acctggcgca gttaacagca ccagcgccat aatatcggcg gcaacgctga
    88561 ccacaatttt gcgtgatttc agttcgctaa taagcgcggt gtagtcgtga atttcaccag
    88621 tagtgccgac ctgctgtaac agcacgccga aaacgtcctg atggtcgagc actttttgcg
    88681 cgtcatcgac aatcacttca aaaccaaagg tttcggcacg agtacggacc acatccagcg
    88741 tttgcggatg aacgtcagaa gcaacaaaga agcggttggc atttttcagt ttgctgacgc
    88801 gtttcgccat cgccattgct tcggcggcag cggtggcttc gtcaagaaga gaagcagagg
    88861 ccatatccag cccggttaaa tccagcgtta cctgctggaa gttgagcagt gcttcaaggc
    88921 ggccctggga gacttcaggt tgatacggag tatacgcggt ataccagccc ggattttcca
    88981 gcatgttacg caggataacc ggcggtagct gcacggcggt gtaacccatg ccgatgtaag
    89041 acgtgaagcg tttattgcga ctggcaatag ccttgagttc tgccagtgcg gcgtattcgg
    89101 tcgccggtgc gccaacctgc ggcggagtcg caagctgaat atctttcggc acaatctggc
    89161 cggtcagcgc gtttaacgat tgtgcgccaa cggcattcag catttcttgc tgttgcgcgg
    89221 cgtccggtcc gatatggcgt tcaataaaag cgccgctgtt ttcaagctgg cttaacgtct
    89281 gtgtcatgag cgatggttcc tgaaacgtgc agtgaattgt gaacctctct ccttgcgaag
    89341 agagtgaggg tgaggcgtaa atttcctcac cctgatcctc tcccgcagaa gaggaataaa
    89401 gccgttactc gtcttctaac aatgcttcgt atgcggttgc atccagcagt gattccagtt
    89461 cgctttcatc gctggctttg attttgaaaa tccagccgcc tgcatacggt tcgctgttca
    89521 ccagttccgg ggaatcgctc agtgcatcgt ttaccgccac gatttcaccg cttactggcg
    89581 cataaatgtc tgacgccgct tttaccgatt cggcaaccgc gcagtcatcg cccgcgctaa
    89641 ccgttgcgcc cacttccggt aggtcaacaa acaccatatc gcctaacagc tcctgagcgt
    89701 gttcggtgat gccaacggtg taagtgccgt cggcttcttt acgcaaccat tcgtgttctt
    89761 tgctgtattt cagttctgct ggtatgttgc tcatcaatca atctccaaaa aagtaaatca
    89821 cgcgacggct ttgccgttac gcacaaaaac aggtttcgtc actttaaccg gcatttcacg
    89881 gttgcgaatt tgcacaatcg ccgtttcacc aataccttcc ggcacgcgcg ccagcgcaat
    89941 gctataaccc agcgttgggg agaaagtacc gctggtgata atgccttcat gctggttgcc
    90001 ctgcgcatca gtaaaacgta ccggcagttc attacgcagc acgccttttt cggtcatcac
    90061 cagaccaacc agtttttctg tgccatgttc acgctgcgct tccagagctt cacgaccgat
    90121 aaagtcacga tctgccggtt cccaggcgat agtccagccc atattggcgg ctaaaggaga
    90181 aatagtttcg tccatctcct ggctatacaa attcatgccc gcttccagac gcagcgtgtc
    90241 acgcgcgccc aggccacatg gcttaacgcc agcttccacc agcgcgcgcc agaaatcggc
    90301 cgctttttca ttgggcagcg caatttcgta gcccgcttca ccggtgtaac cggtggtggc
    90361 aataaacaga tcgcccgcct gcacgccaaa gaacggtttc atcccttcca ccgcctgacg
    90421 ctgggcgtca ttaaacagtg tggcagcttt tgcctgcgca ttcggccctt gtacggcgat
    90481 catggaaagg tcatcacgaa cagtaatttc gatgccgaaa ggttcagcgt gttgggtaat
    90541 ccaggagagg tctttttcgc gggtggcgga gttaacaacg aggcggaaga aatcttcagt
    90601 aaagtagtag acgatgagat catctatcac accgccagag gcattcaaca tccccgagta
    90661 aagggctttg ccgcttttgg tgagcttcgc cacatcgttt gccagcagat aacgtagaaa
    90721 ctcccgggtg cggctgccgc gaagatcgac gatggtcata tgtgacacat caaacattcc
    90781 ggcatcggta cgtaccgcat gatgttcgtc gatttgcgaa ccgtaatgca gcggcatcat
    90841 ccagccgtgg aaatccacca tgcgagcgcc gcaaagcgtg tgttgttcgt acaaaggagt
    90901 ctgttgtgcc atcttgtcct cattgaataa gcggggctga caacttttct atggcgaaat
    90961 tatcaccaca aaacccagca tcggagccac tcccggtccc caacgcaatc gttctctttt
    91021 gcctgaactt accaccgaaa cagactgtta accataaggt taaattgatc atcacattag
    91081 cttatggtta aaaaacgcca aaatcgtaac agaataaaaa accaaaaaat gaacaaatc
//
LOCUS       DAZKHK010000002        86919 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKHK010000002 DAZKHK010000000
VERSION     DAZKHK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.002"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..513
                     /gene="agaB"
                     /locus_tag="ACHOS5_000074"
     CDS             37..513
                     /gene="agaB"
                     /locus_tag="ACHOS5_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP9774972.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            552..1355
                     /gene="agaC"
                     /locus_tag="ACHOS5_000075"
     CDS             552..1355
                     /gene="agaC"
                     /locus_tag="ACHOS5_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP9774973.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1345..2136
                     /gene="agaD"
                     /locus_tag="ACHOS5_000076"
     CDS             1345..2136
                     /gene="agaD"
                     /locus_tag="ACHOS5_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP9774974.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2137..2904
                     /gene="agaI"
                     /locus_tag="ACHOS5_000077"
     CDS             2137..2904
                     /gene="agaI"
                     /locus_tag="ACHOS5_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP9774975.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVYTAIPASFLWLHNYFTCMIDEMCRR"
     gene            complement(2954..3817)
                     /gene="rsmI"
                     /locus_tag="ACHOS5_000078"
     CDS             complement(2954..3817)
                     /gene="rsmI"
                     /locus_tag="ACHOS5_000078"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9774976.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3882..5918
                     /gene="lpoA"
                     /locus_tag="ACHOS5_000079"
     CDS             3882..5918
                     /gene="lpoA"
                     /locus_tag="ACHOS5_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP9774977.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDTQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADEIILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQSQPQTVDDVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5876..6271
                     /gene="yraN"
                     /locus_tag="ACHOS5_000080"
     CDS             5876..6271
                     /gene="yraN"
                     /locus_tag="ACHOS5_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP9774978.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6291..6881
                     /gene="diaA"
                     /locus_tag="ACHOS5_000081"
     CDS             6291..6881
                     /gene="diaA"
                     /locus_tag="ACHOS5_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP9774979.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6891..7466
                     /gene="dolP"
                     /locus_tag="ACHOS5_000082"
     CDS             6891..7466
                     /gene="dolP"
                     /locus_tag="ACHOS5_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9774980.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7579..8619)
                     /gene="yraQ"
                     /locus_tag="ACHOS5_000083"
     CDS             complement(7579..8619)
                     /gene="yraQ"
                     /locus_tag="ACHOS5_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9774981.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPESQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8692..9327)
                     /gene="yraR"
                     /locus_tag="ACHOS5_000084"
     CDS             complement(8692..9327)
                     /gene="yraR"
                     /locus_tag="ACHOS5_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9774982.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9455..9973
                     /gene="yhbO"
                     /locus_tag="ACHOS5_000085"
     CDS             9455..9973
                     /gene="yhbO"
                     /locus_tag="ACHOS5_000085"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP9774983.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9953..10396)
                     /gene="yhbP"
                     /locus_tag="ACHOS5_000086"
     CDS             complement(9953..10396)
                     /gene="yhbP"
                     /locus_tag="ACHOS5_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP9774984.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10447..10749
                     /gene="yhbQ"
                     /locus_tag="ACHOS5_000087"
     CDS             10447..10749
                     /gene="yhbQ"
                     /locus_tag="ACHOS5_000087"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP9774985.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAVFAELLNSLQTPEIKSD"
     gene            complement(10736..11239)
                     /gene="yhbS"
                     /locus_tag="ACHOS5_000088"
     CDS             complement(10736..11239)
                     /gene="yhbS"
                     /locus_tag="ACHOS5_000088"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9774986.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11233..11757)
                     /gene="ubiT"
                     /locus_tag="ACHOS5_000089"
     CDS             complement(11233..11757)
                     /gene="ubiT"
                     /locus_tag="ACHOS5_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP9774987.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11966..12961
                     /gene="ubiU"
                     /locus_tag="ACHOS5_000090"
     CDS             11966..12961
                     /gene="ubiU"
                     /locus_tag="ACHOS5_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9774988.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVNGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12970..13848
                     /gene="ubiV"
                     /locus_tag="ACHOS5_000091"
     CDS             12970..13848
                     /gene="ubiV"
                     /locus_tag="ACHOS5_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9774989.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13929..14936
                     /gene="yhbW"
                     /locus_tag="ACHOS5_000092"
     CDS             13929..14936
                     /gene="yhbW"
                     /locus_tag="ACHOS5_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFP9774990.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15054..16298)
                     /gene="mtr"
                     /locus_tag="ACHOS5_000093"
     CDS             complement(15054..16298)
                     /gene="mtr"
                     /locus_tag="ACHOS5_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP9774991.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(16452..18341)
                     /gene="deaD"
                     /locus_tag="ACHOS5_000094"
     CDS             complement(16452..18341)
                     /gene="deaD"
                     /locus_tag="ACHOS5_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP9774992.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18334..18414)
                     /gene="yrbN"
                     /locus_tag="ACHOS5_000095"
     CDS             complement(18334..18414)
                     /gene="yrbN"
                     /locus_tag="ACHOS5_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP9774993.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18521..19405)
                     /gene="nlpI"
                     /locus_tag="ACHOS5_000096"
     CDS             complement(18521..19405)
                     /gene="nlpI"
                     /locus_tag="ACHOS5_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP9774994.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19514..21649)
                     /gene="pnp"
                     /locus_tag="ACHOS5_000097"
     CDS             complement(19514..21649)
                     /gene="pnp"
                     /locus_tag="ACHOS5_000097"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9774995.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21896..22165)
                     /gene="rpsO"
                     /locus_tag="ACHOS5_000098"
     CDS             complement(21896..22165)
                     /gene="rpsO"
                     /locus_tag="ACHOS5_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9774996.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22314..23258)
                     /gene="truB"
                     /locus_tag="ACHOS5_000099"
     CDS             complement(22314..23258)
                     /gene="truB"
                     /locus_tag="ACHOS5_000099"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9774997.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23258..23659)
                     /gene="rbfA"
                     /locus_tag="ACHOS5_000100"
     CDS             complement(23258..23659)
                     /gene="rbfA"
                     /locus_tag="ACHOS5_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9774998.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23823..26495)
                     /gene="infB"
                     /locus_tag="ACHOS5_000101"
     CDS             complement(23823..26495)
                     /gene="infB"
                     /locus_tag="ACHOS5_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9774999.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26520..28007)
                     /gene="nusA"
                     /locus_tag="ACHOS5_000102"
     CDS             complement(26520..28007)
                     /gene="nusA"
                     /locus_tag="ACHOS5_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9775000.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(28035..28487)
                     /gene="rimP"
                     /locus_tag="ACHOS5_000103"
     CDS             complement(28035..28487)
                     /gene="rimP"
                     /locus_tag="ACHOS5_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9775001.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(28694..28770)
                     /locus_tag="ACHOS5_000104"
     tRNA            complement(28694..28770)
                     /locus_tag="ACHOS5_000104"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28734..28736),aa:Met,seq:cat)
     gene            29118..30461
                     /gene="argG"
                     /locus_tag="ACHOS5_000105"
     CDS             29118..30461
                     /gene="argG"
                     /locus_tag="ACHOS5_000105"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP9775002.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(30469..32094)
                     /gene="yhbX"
                     /locus_tag="ACHOS5_000106"
     CDS             complement(30469..32094)
                     /gene="yhbX"
                     /locus_tag="ACHOS5_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9775003.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAVPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFTFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(32551..32637)
                     /locus_tag="ACHOS5_000107"
     tRNA            complement(32551..32637)
                     /locus_tag="ACHOS5_000107"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32601..32603),aa:Leu,seq:gag)
     gene            complement(32652..32984)
                     /gene="secG"
                     /locus_tag="ACHOS5_000108"
     CDS             complement(32652..32984)
                     /gene="secG"
                     /locus_tag="ACHOS5_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9775004.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(33212..34549)
                     /gene="glmM"
                     /locus_tag="ACHOS5_000109"
     CDS             complement(33212..34549)
                     /gene="glmM"
                     /locus_tag="ACHOS5_000109"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9775005.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(34542..35390)
                     /gene="folP"
                     /locus_tag="ACHOS5_000110"
     CDS             complement(34542..35390)
                     /gene="folP"
                     /locus_tag="ACHOS5_000110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP9775006.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYHDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(35480..37414)
                     /gene="ftsH"
                     /locus_tag="ACHOS5_000111"
     CDS             complement(35480..37414)
                     /gene="ftsH"
                     /locus_tag="ACHOS5_000111"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9775007.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(37514..38143)
                     /gene="rlmE"
                     /locus_tag="ACHOS5_000112"
     CDS             complement(37514..38143)
                     /gene="rlmE"
                     /locus_tag="ACHOS5_000112"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP9775008.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            38269..38562
                     /gene="yhbY"
                     /locus_tag="ACHOS5_000113"
     CDS             38269..38562
                     /gene="yhbY"
                     /locus_tag="ACHOS5_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9775009.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(38718..39194)
                     /gene="greA"
                     /locus_tag="ACHOS5_000114"
     CDS             complement(38718..39194)
                     /gene="greA"
                     /locus_tag="ACHOS5_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9775010.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            39442..40875
                     /gene="dacB"
                     /locus_tag="ACHOS5_000115"
     CDS             39442..40875
                     /gene="dacB"
                     /locus_tag="ACHOS5_000115"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9775011.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSISLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40916..42088)
                     /gene="cgtA"
                     /locus_tag="ACHOS5_000116"
     CDS             complement(40916..42088)
                     /gene="cgtA"
                     /locus_tag="ACHOS5_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP9775012.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(42104..43069)
                     /gene="yhbE"
                     /locus_tag="ACHOS5_000117"
     CDS             complement(42104..43069)
                     /gene="yhbE"
                     /locus_tag="ACHOS5_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9775013.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(43196..43453)
                     /gene="rpmA"
                     /locus_tag="ACHOS5_000118"
     CDS             complement(43196..43453)
                     /gene="rpmA"
                     /locus_tag="ACHOS5_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9775014.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(43474..43785)
                     /gene="rplU"
                     /locus_tag="ACHOS5_000119"
     CDS             complement(43474..43785)
                     /gene="rplU"
                     /locus_tag="ACHOS5_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9775015.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            44044..45015
                     /gene="ispB"
                     /locus_tag="ACHOS5_000120"
     CDS             44044..45015
                     /gene="ispB"
                     /locus_tag="ACHOS5_000120"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP9775016.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            45250..45528
                     /gene="sfsB"
                     /locus_tag="ACHOS5_000121"
     CDS             45250..45528
                     /gene="sfsB"
                     /locus_tag="ACHOS5_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP9775017.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(45576..46835)
                     /gene="murA"
                     /locus_tag="ACHOS5_000122"
     CDS             complement(45576..46835)
                     /gene="murA"
                     /locus_tag="ACHOS5_000122"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9775018.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(46890..47144)
                     /gene="ibaG"
                     /locus_tag="ACHOS5_000123"
     CDS             complement(46890..47144)
                     /gene="ibaG"
                     /locus_tag="ACHOS5_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP9775019.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(47304..47597)
                     /gene="mlaB"
                     /locus_tag="ACHOS5_000124"
     CDS             complement(47304..47597)
                     /gene="mlaB"
                     /locus_tag="ACHOS5_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP9775020.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(47597..48232)
                     /gene="mlaC"
                     /locus_tag="ACHOS5_000125"
     CDS             complement(47597..48232)
                     /gene="mlaC"
                     /locus_tag="ACHOS5_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP9775021.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(48251..48802)
                     /gene="mlaD"
                     /locus_tag="ACHOS5_000126"
     CDS             complement(48251..48802)
                     /gene="mlaD"
                     /locus_tag="ACHOS5_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP9775022.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(48807..49589)
                     /gene="mlaE"
                     /locus_tag="ACHOS5_000127"
     CDS             complement(48807..49589)
                     /gene="mlaE"
                     /locus_tag="ACHOS5_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP9775023.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(49597..50406)
                     /gene="mlaF"
                     /locus_tag="ACHOS5_000128"
     CDS             complement(49597..50406)
                     /gene="mlaF"
                     /locus_tag="ACHOS5_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP9775024.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            50616..51593
                     /gene="yrbG"
                     /locus_tag="ACHOS5_000129"
     CDS             50616..51593
                     /gene="yrbG"
                     /locus_tag="ACHOS5_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP9775025.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            51607..52593
                     /gene="kdsD"
                     /locus_tag="ACHOS5_000130"
     CDS             51607..52593
                     /gene="kdsD"
                     /locus_tag="ACHOS5_000130"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP9775026.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            52614..53180
                     /gene="kdsC"
                     /locus_tag="ACHOS5_000131"
     CDS             52614..53180
                     /gene="kdsC"
                     /locus_tag="ACHOS5_000131"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP9775027.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            53177..53752
                     /gene="lptC"
                     /locus_tag="ACHOS5_000132"
     CDS             53177..53752
                     /gene="lptC"
                     /locus_tag="ACHOS5_000132"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP9775028.1"
                     /translation="MSKARRWVIIVLSLGVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            53721..54278
                     /gene="lptA"
                     /locus_tag="ACHOS5_000133"
     CDS             53721..54278
                     /gene="lptA"
                     /locus_tag="ACHOS5_000133"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP9775029.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            54285..55010
                     /gene="lptB"
                     /locus_tag="ACHOS5_000134"
     CDS             54285..55010
                     /gene="lptB"
                     /locus_tag="ACHOS5_000134"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP9775030.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            55058..56491
                     /gene="rpoN"
                     /locus_tag="ACHOS5_000135"
     CDS             55058..56491
                     /gene="rpoN"
                     /locus_tag="ACHOS5_000135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9775031.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            56514..56801
                     /gene="hpf"
                     /locus_tag="ACHOS5_000136"
     CDS             56514..56801
                     /gene="hpf"
                     /locus_tag="ACHOS5_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP9775032.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56919..57410
                     /gene="ptsN"
                     /locus_tag="ACHOS5_000137"
     CDS             56919..57410
                     /gene="ptsN"
                     /locus_tag="ACHOS5_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP9775033.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            57456..58310
                     /gene="rapZ"
                     /locus_tag="ACHOS5_000138"
     CDS             57456..58310
                     /gene="rapZ"
                     /locus_tag="ACHOS5_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9775034.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            58307..58579
                     /gene="npr"
                     /locus_tag="ACHOS5_000139"
     CDS             58307..58579
                     /gene="npr"
                     /locus_tag="ACHOS5_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP9775035.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            58792..59424
                     /gene="yrbL"
                     /locus_tag="ACHOS5_000140"
     CDS             58792..59424
                     /gene="yrbL"
                     /locus_tag="ACHOS5_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP9775036.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(59421..60149)
                     /gene="mtgA"
                     /locus_tag="ACHOS5_000141"
     CDS             complement(59421..60149)
                     /gene="mtgA"
                     /locus_tag="ACHOS5_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP9775037.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LARNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(60146..60799)
                     /gene="elbB"
                     /locus_tag="ACHOS5_000142"
     CDS             complement(60146..60799)
                     /gene="elbB"
                     /locus_tag="ACHOS5_000142"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP9775038.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(61029..63365)
                     /gene="arcB"
                     /locus_tag="ACHOS5_000143"
     CDS             complement(61029..63365)
                     /gene="arcB"
                     /locus_tag="ACHOS5_000143"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP9775039.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRALTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(63461..64390)
                     /gene="yhcC"
                     /locus_tag="ACHOS5_000144"
     CDS             complement(63461..64390)
                     /gene="yhcC"
                     /locus_tag="ACHOS5_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9775040.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVGLDRYLNEHGVQGSALGRPWLPPTA"
     gene            65065..69525
                     /gene="gltB"
                     /locus_tag="ACHOS5_000145"
     CDS             65065..69525
                     /gene="gltB"
                     /locus_tag="ACHOS5_000145"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP9775041.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGQLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            69538..70956
                     /gene="gltD"
                     /locus_tag="ACHOS5_000146"
     CDS             69538..70956
                     /gene="gltD"
                     /locus_tag="ACHOS5_000146"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP9775042.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMARRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            71140..72267
                     /gene="yhcG"
                     /locus_tag="ACHOS5_000147"
     CDS             71140..72267
                     /gene="yhcG"
                     /locus_tag="ACHOS5_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP9775043.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRPSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLILCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(72327..72791)
                     /gene="nanQ"
                     /locus_tag="ACHOS5_000148"
     CDS             complement(72327..72791)
                     /gene="nanQ"
                     /locus_tag="ACHOS5_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP9775044.1"
                     /translation="MMMGEVQSLPSAGLHPALQGALTLALAARPQEKASGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKIKADLMA"
     gene            complement(72788..73663)
                     /gene="nanK"
                     /locus_tag="ACHOS5_000149"
     CDS             complement(72788..73663)
                     /gene="nanK"
                     /locus_tag="ACHOS5_000149"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9775045.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(73660..74349)
                     /gene="nanE"
                     /locus_tag="ACHOS5_000150"
     CDS             complement(73660..74349)
                     /gene="nanE"
                     /locus_tag="ACHOS5_000150"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9775046.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDMEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(74397..75887)
                     /gene="nanT"
                     /locus_tag="ACHOS5_000151"
     CDS             complement(74397..75887)
                     /gene="nanT"
                     /locus_tag="ACHOS5_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP9775047.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(75996..76889)
                     /gene="nanA"
                     /locus_tag="ACHOS5_000152"
     CDS             complement(75996..76889)
                     /gene="nanA"
                     /locus_tag="ACHOS5_000152"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9775048.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(77011..77802)
                     /gene="nanR"
                     /locus_tag="ACHOS5_000153"
     CDS             complement(77011..77802)
                     /gene="nanR"
                     /locus_tag="ACHOS5_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9775049.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(77910..78407)
                     /gene="sspB"
                     /locus_tag="ACHOS5_000154"
     CDS             complement(77910..78407)
                     /gene="sspB"
                     /locus_tag="ACHOS5_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP9775050.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(78413..79051)
                     /gene="sspA"
                     /locus_tag="ACHOS5_000155"
     CDS             complement(78413..79051)
                     /gene="sspA"
                     /locus_tag="ACHOS5_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP9775051.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(79446..79838)
                     /gene="rpsI"
                     /locus_tag="ACHOS5_000156"
     CDS             complement(79446..79838)
                     /gene="rpsI"
                     /locus_tag="ACHOS5_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9775052.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(79854..80282)
                     /gene="rplM"
                     /locus_tag="ACHOS5_000157"
     CDS             complement(79854..80282)
                     /gene="rplM"
                     /locus_tag="ACHOS5_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9775053.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(80502..81629)
                     /gene="zapE"
                     /locus_tag="ACHOS5_000158"
     CDS             complement(80502..81629)
                     /gene="zapE"
                     /locus_tag="ACHOS5_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP9775054.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            81823..82221
                     /gene="zapG"
                     /locus_tag="ACHOS5_000159"
     CDS             81823..82221
                     /gene="zapG"
                     /locus_tag="ACHOS5_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP9775055.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            82375..83742
                     /gene="degQ"
                     /locus_tag="ACHOS5_000160"
     CDS             82375..83742
                     /gene="degQ"
                     /locus_tag="ACHOS5_000160"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP9775056.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            83832..84899
                     /gene="degS"
                     /locus_tag="ACHOS5_000161"
     CDS             83832..84899
                     /gene="degS"
                     /locus_tag="ACHOS5_000161"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP9775057.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(84962..85900)
                     /gene="mdh"
                     /locus_tag="ACHOS5_000162"
     CDS             complement(84962..85900)
                     /gene="mdh"
                     /locus_tag="ACHOS5_000162"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9775058.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVLGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            86335..86805
                     /gene="argR"
                     /locus_tag="ACHOS5_000163"
     CDS             86335..86805
                     /gene="argR"
                     /locus_tag="ACHOS5_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP9775059.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        21323 a        23471 c        21632 g        20493 t
ORIGIN      
        1 gattatatta ttcaacttta tggtgaggat tacacaatga gcagtccaaa tattctctta
       61 acccgtattg ataaccgtct ggttcacggt caggttggcg tgacctggac atccaccatc
      121 ggtgcgaatc tgttagtagt cgtggatgat gttgtcgcta acgatgatat tcaacagaaa
      181 ttaatgggta ttaccgcaga aacctacggc tttggcattc gtttctttac tatcgaaaaa
      241 accattaacg ttatcggcaa agctgcacca catcagaaga tcttcctgat ttgccgtacg
      301 ccacaaacgg tacgtaaatt ggtagaaggt ggtattgacc tgaaagatgt caacgtcggc
      361 aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt cgatgaccag
      421 gatctcacgg atttacgttt tattaaacaa cgtggcgtaa atgttttcat tcaggacgtc
      481 cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa tattggtttg
      541 aggtaataaa aatgcatgaa ataaccctac tccagggatt atccctggcg gcgttagttt
      601 tctttctggg gattgatttt tggctggaag ccttattttt attccgcccg ataatcgttt
      661 gtacccttac aggtgctatt ctcggtgata ttcagactgg cttaattacc ggtggcctga
      721 cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc aaccctatta
      781 tggcgggtct gatgaccact gtcattgcat ggtctacggg cgttgatgcc aaaacggcaa
      841 ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc ttctattccg
      901 ctttctcatt atttatgacc aaagccgata aatgcgcaaa agaggcggat acggcagcgt
      961 tttcccgact taactggaca acgatgctca tcgtcgcttc agcgtatgcg gtgattgctt
     1021 tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg atgcccgcct
     1081 ggctgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt tttggcttgc
     1141 tgctgcgcgt gatgttcaaa gcgcaatata tcccttacct gatcgccggt ttcctgttcg
     1201 tttgctacat ccaggtcagc aacctgttgc cggttgccgt actgggcgca ggctttgcgg
     1261 tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc gttgccagta
     1321 aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga tatcacccgt
     1381 ctgggctttc gttcgtcact gctgcaagcg agctttaact acgaaaggat gcaggcgggc
     1441 ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggacga caaaccgggc
     1501 ctaagcgcgg cgatgaaaga taacctcgaa tttattaata cccacccgaa tctggtcgga
     1561 ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga caccattaaa
     1621 ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat tttctggttt
     1681 accttattgc cgattatggc gggaatttgc tcatcatttg ccagccaggg aaacttgctg
     1741 gggccgattc tatttttcgc cgtttacctg cttatcttct tcctgcgcgt cggctggacc
     1801 cacgtcggtt attcagtcgg cgtgaaggct attgataaag tgcgagagaa ctcgcagatg
     1861 attgctcgtt cggcaaccat cctcgggatc acggtaatcg gcgggctgat cgcttcgtat
     1921 gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt cgcgctgcag
     1981 caggatttct ttgataaagt cttcccgaac attttaccga tggcctacac cctgctgatg
     2041 tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt gacgtttgtt
     2101 ctttctattg tttgttctgc attcggcatt ttgtaaatgg aacgaggcac tgcgtctggc
     2161 ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcaacaagt tgaaaactat
     2221 acggcgttaa gtgaacgtgc cagcgaatat ttattggccg tgatccgtag taaaccggat
     2281 gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta tctggtagaa
     2341 aaaatccacc agcagcaggt tgatgtcagc cagctcacct tcgtgaagct cgacgaatgg
     2401 gtggatctgc cattaacaat gccaggcacc tgcgaaactt tcctacaaca gcatatcgtg
     2461 cagccgctgg ggctgcgtga agaccagctc atcagctttc gctctgaaga gataaatgag
     2521 acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga tttatgcgtt
     2581 ctcggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaaa cctgcaaccg
     2641 gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt aaaaaccgcg
     2701 ggccgtcctg tgactcgtgg gatcacctta ggcctgaagg atattctcaa tgcccgcgaa
     2761 gttttgttac tggtgactgg cgaaggaaaa caggatgcga cagaacgttt tctcacggct
     2821 aaagtctata ccgctatccc ggcttcattt ttatggctgc ataactattt cacatgcatg
     2881 attgatgaga tgtgtcgtcg ttaactcgcc tgctacaggc gttgcaacgc tattcctgct
     2941 gcgacgcctg taatcactct ccctgctgct ccagcgcata cttatacagc gcatttttct
     3001 tcacgccgtg aatctctgct gccagcgccg ccgctttttt cagcggcagt tctgcctgca
     3061 acagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatggcctt
     3121 cgacaatcag caccatttcg cctttgcgac ggttttcatc ttctttgacc cacgccagca
     3181 gctcgccaac gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa
     3241 ccacgtagcg ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt
     3301 gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac
     3361 ggcggccttt tgatttggca ggtaaaaagc cttcataaca aaaacgatcg gatggtaaac
     3421 ccgcggcgct taacgcagtg atagcagcac acggcccggg tagcggcacc acgcgaatcc
     3481 ccgcttcacg gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg
     3541 catcggaaac cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt
     3601 tttgttgttc attatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca
     3661 gcaataaacc ggtgtgacgc gtatcctcgg cggcaatcag atcaacggcc tgtaatacct
     3721 ctaatgcacg ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct
     3781 ggccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt
     3841 aatattgagc attgcgtaaa aaaaatatca ctggatacat tatggtaccc tcaacatttt
     3901 ctcgtttgaa agccgcgcgt tgtctgcctg ttgttctggc agccctgatt ttcgccggtt
     3961 gtggcaccca tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt
     4021 ctgcctttta cctgcaacag atgcagcaaa gttctgatga taccaggatc aactggcaat
     4081 tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta
     4141 accaactacc gcaagaactg aatgatactc agcgtcgcga gaaaacactg ctagcggcag
     4201 agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac
     4261 ctgccgattt agaacaaaac cagcaagccc gttactggca ggcaaaaatc gatgccagcc
     4321 aggggcgtcc ttccattgat ttactgcgcg cgttaattgc tcaggaaccg ctgctcggtg
     4381 cgaaagaaaa gcagcagaat attgacgcca catggcaggc gctctcctcc atgactcagg
     4441 agcaggcgaa tacgctggtg atcaacgccg acgaaattat tctgcaaggc tggctggatt
     4501 tgcagcgcgt ctggtttgat aaccgtaacg atcctgacat gatgaaagct gggatcgccg
     4561 actggcagaa acgttatccg aacaacccgg gcgcgaaaat gctgccaacg cagttggtta
     4621 acgtaaaagc gtttaaacca gcatcgacca acaaaatcgc cctgctgttg ccgctgaatg
     4681 gccaggcagc ggtatttggt cgcactattc agcaaggctt tgaagcggcg aaaaatatcg
     4741 gcactcagcc agtggcggcc caggtagctg ccgcacctgc cgcagacgta gcagaacaat
     4801 ctcagccgca aaccgtggat gacgttgcca gcccggcaca agcctcggtt agcgatctga
     4861 ccggtgaaca gcctgcggcc cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag
     4921 cggtaagcgc acccgcaaat ccatccgcag agctgaaaat ctacgatacc tcatcacaac
     4981 cacttagcca gatcttaagc caggttcagc aggatggcgc gagtattgtg gtcggtccgt
     5041 tgctgaaaaa taacgttgaa gagttgctga agagcaacac tccgctgaac gtgctggcac
     5101 tgaaccagcc ggagaacatc gaaaaccgcg tcaatatttg ttacttcgcg ctttcaccgg
     5161 aagacgaagc gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg
     5221 tgctgatccc acgcagttca ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc
     5281 agaaactggg cggcggcacc gttctgcaac aaaaatttgg ttccaccagc gaattacgcg
     5341 cgggtgttaa cggcggttcg ggtatcgctt taacgggtag cccgattact cccagagaaa
     5401 caaccgactc cggcatgacg accaacaatc caacgctgca aaccacgcca accgatgatc
     5461 agttcaccaa taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa
     5521 tcgcttttat caaaccaatg atcgccatgc gtaacggtag ccagagcggt gcaacgctgt
     5581 acgccagctc ccgcagtgcg caagggaccg ctggcccgga tttccgcctg gagatggaag
     5641 gcttgcagta cagcgaaatc ccgatgctgg caggcggtaa tctgccgtta atgcagcagg
     5701 cgctcagcgc ggtgaacaac gattattcgc tggctcgcat gtatgcgatg ggcgtcgatg
     5761 cctggtcgtt ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg
     5821 gtaataccgg aagcctgacg gcaaacccgg attgcgtgat taacaggaag ttatcatggc
     5881 tacagtacca acaaggtcag gtagtccccg ccagttaacc accaaacaga ccggcgatgc
     5941 gtgggaagca caagcgcgtc gctggctgga aggcaaagga ctacggttta tcgccgctaa
     6001 cgtgaacgag cgtggcggcg agatcgatct gataatgcgt gaaggccgga ccaccatttt
     6061 tatcgaggtg cgttaccgcc gctctgcgct ttatggcggc gcggcagcca gtgtgacccg
     6121 cagcaaacaa cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag
     6181 ttttgatact gtggattgcc ggttcgatgt ggtagccttc accgggaatg aggttgagtg
     6241 gattaaggat gcctttaatg accactcata attaaggttt aaggattagc gtgcaagaaa
     6301 gaattaaagc ttgcttcact gaaagcattc aaactcaaat tgcggcggca gaggcgcttc
     6361 cggatgccat ctcccgtgca gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa
     6421 tcctctgttg tggtaatgga acttccgctg ccaatgcaca gcattttgct gccagcatga
     6481 tcaaccgttt cgaaacggag cggcccagct tacctgccat tgcactaaat actgataatg
     6541 ttgtcttaac ggcaattgcc aacgatcgct tacatgatga agtgtatgca aaacaggtgc
     6601 gggcgctggg tcatgcggga gatgtattgt tagccatttc cacccgtggc aacagccgcg
     6661 atattgtaaa agcggtagaa gctgccgtta cgcgtgatat gaccattgtg gcattaaccg
     6721 gctatgacgg cggtgaactt gcaggtttgt tagggccaca ggatgtggag atccgcattc
     6781 cttcgcatcg tagtgctcgc attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg
     6841 atctgatcga taacacgctt ttccctcacc aggatgttta aggagaatac atgaaggcat
     6901 tatcgccaat cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt gccgctgccg
     6961 tagtgggtac tgctgctgtg ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc
     7021 aggtagacga tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaaa
     7081 ttaagaaaga agcgcgcatt aatgtaacgg cctatcaggg caaagtgctg ctggttgggc
     7141 agtcaccaaa cgctgaactt tcggctcgcg ccaaacagat tgctatgggc gtagacggtg
     7201 ccaacgaagt gtataacgag attcgtcagg gccagccgat tggtctgggc gaagcgtcta
     7261 acgatacgtg gatcaccacc aaagtacgtt cgcagctgtt aaccagcgac ctggtgaaat
     7321 cgtccaacgt gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg
     7381 aacgtgaagc gaaagcggcg gcagatattg ccagccgggt aagcggcgtg aagcgggtaa
     7441 ctacggcgtt tacgtttatt aaatagcagc ccttgtaatg cctgatgcga cgcttacagc
     7501 gtcttatcag gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc
     7561 gacatgtttt ccctcaaatc aaaccaacag cgccagcccg ccgacaatca caccagacac
     7621 tgccaccatc gcccccgtca gccataaggc tttcgccgga aacgctttac gcaacattat
     7681 cagcgacggc aaactcaccg ccgggagcgt catcaacaat gccagcgccg gagcggttcc
     7741 catacctgcc agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa
     7801 caagcatccc gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc
     7861 ggcatggggg aataaccaga cgcgagcggc acccaacacc agtactgcaa ggatgtaaac
     7921 cgggatcgtg ctccagaaaa gcgtccatag cgccctgccc cagcggctaa aaaaaccgcc
     7981 ctgtgattcc ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca
     8041 tttttgcacc agcgtcgcaa tcagcaacac catcaccagc cccgccacca gacgaatcgc
     8101 cgcaaaaccc cagccgagga cgaaccccat aaacaccagc gtcgccgggt ttaacaacgg
     8161 attccccatc cagaatgcca gcgcaccgcc catcgacacc tgttggcgac gcattcccgc
     8221 cgcgaccgga gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag
     8281 cagcgtgccg cgaaagcgcg attgcccaag cgtacgcaac aaccagtcac gcgggatcag
     8341 cacctgaatc aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgagaaa
     8401 atagatcatc gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat
     8461 agatttaccg atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca
     8521 tttcacatac cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg
     8581 aatgggcgtt gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt
     8641 atgataattg agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc
     8701 tcttttgcgt aattctgaag agcttaaaat cgtcacacct tcatgctcag gccgcatcgc
     8761 ctctgccagc atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggtaa
     8821 caaacggaac agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag
     8881 taacatcgac gggcgagcaa tggtcaattt cggccagttc tgggcgatta atgcttcttc
     8941 catctcccct ttgacgcggt tatagaaaaa cggcgagtgg gcattagcac ccatagcact
     9001 gaccaccagc atatgctgcg caccgagccg ccgcccggtt aatgcggtat ccactaccaa
     9061 cgtgtaatcg gcatgaataa acgcctcttt gctccccgct tcccgccgcg tggtgccgag
     9121 acaacaaaat acaatgtcga tgggatcggt gacctgcgcc agcgcgtcgg tcagttgcgg
     9181 gtcatgggga ttaaacaccc ctggcatatc gcccaacggg cgtcgcgtcg gcgcggctat
     9241 ggcgttaact ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt
     9301 tgcgcctgta atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc
     9361 tccccggctc atgcaccagg cttaatagtc tacaaggtaa ttatccccga tcgtgagaaa
     9421 ttcgcctcat ccaatgcaac aacgcggagg aaacatgagt aagaagattg ccgttttaat
     9481 cactgatgaa tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg
     9541 acacgaagtg attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga
     9601 agccagcgtg accatcgata aatccatcga tgaagtgacg cccgcagagt tcgatgccct
     9661 gctgctaccg ggcggtcatt caccagatta tctgcgtggt gacaaccgtt ttgtcacctt
     9721 cacacgcgat tttgtgaaca gcggcaaacc tgttttcgcc atctgtcacg gtccccagtt
     9781 gctgatcagc gccgatgtga tccgtggtcg taagctgacc gccgttaaac cgatcgtgat
     9841 tgatgttaaa aacgcgggcg cggaatttta cgatcaggaa gtggtggtcg ataaagatca
     9901 gctggttacc agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct
     9961 gctcggtgcc tgagttacgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg
    10021 tgaatttgat ttcatcgagc cggatttccc ataccggggc cgacagcatt ctggcaaccg
    10081 gaaagcgacg attgtacgcc ttgcgtgcga ggtcgctttc ctcgccttcc agtctgcgga
    10141 tctcaccttt aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg
    10201 ttccggcaat tgccgcctgc ggcccgctca tctgcgcatg acgcgttttt tcttccgtca
    10261 aaatgtagaa agcgactttc tgcgcatcaa aaagataaaa ggcattggcg caccacagtt
    10321 ccccttcctg ctgcacacac caggtgacaa catgttgttt tgccagccaa cggctgatgg
    10381 cggtgagtgt ttccattgct gttctctttt atactgtggg cctgacctta acatgcgacg
    10441 cagacgatga caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc
    10501 gggatcacca cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa
    10561 gcactgcgcg ggaagggaga actaacgctg gcgttttccg caccagtggg cgatcgttcg
    10621 ctggcgttac gggcagaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg
    10681 gtagcggaag gtgcagtgtt tgcagaactg ttgaacagcc tgcaaacccc ggagattaaa
    10741 agcgattgaa gtgctcgtga tactcaacca gaccagttac accgttcagc gcgtcatccg
    10801 ctaaacgatg tacctggaag gcactttcag tgcccggcca gcggcaacgc agatcgtgat
    10861 gtgccgccag ttcaaagccg aaacggctgt acagcgccgg atcgcccagc gtcaccactg
    10921 cggcatagcc gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc
    10981 cctgtccacg gtatttttca tctaccgcca gcggtgccat gccgacccat tgcaggtctt
    11041 cgccctgcac atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg
    11101 tcgccaccag ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt
    11161 ccgcatcact ttcgaatgaa cgacgcagca gggcatcaat acccggcgca tcaatgggaa
    11221 tttctactcg aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt
    11281 cattcccgcc tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat
    11341 ttgctccagt tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc
    11401 ttcaatcacc agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat
    11461 catcagcaga tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctaac
    11521 gaccagtttg ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat
    11581 acttaaccag cgaccttcaa gaaactccag ctcgccatca tccagcgcct gacggaattg
    11641 ccagcttaag acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg
    11701 tacactcaac agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag
    11761 ttttactccc tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt
    11821 ataaatcaac aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa
    11881 atcaaaaatt gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt
    11941 gatagcgcaa ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc
    12001 gcttaaggcg gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac
    12061 caatgcccgt cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag
    12121 ttttgtccat caacatcgcc gcaaacttca catcgcgatt aacacatttg cgcatccgga
    12181 cggttacgcc cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct
    12241 gatcctcgcc gacctcgcca tgctggagta cgccgccgag cgttatccgc atattgagcg
    12301 ccacgtatcg gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca
    12361 ttttgacgtt gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacaact
    12421 ggcacgggtc acacctgtac cactggaagt ctttgctttc ggcagcctgt gcattatgtc
    12481 ggaaggtcgt tgctatctgt cgtcgtattt gacgggtgag tcgcccaaca ccgtgggcgc
    12541 gtgttctccg gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct
    12601 gaacgaagtg ctgatcgacc gttatcagga cggcgaaaac gcaggttatc cgacgctatg
    12661 taaagggcgt tatctggtga acggcgagcg ctatcacgcg ctggaagaac caaccagtct
    12721 caataccctg gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga
    12781 aggccgccag cgtagtccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat
    12841 cgaccgttgt aaggccgatc cgcaaaactt cataccgcaa agcgcgtgga tggagacgct
    12901 cgggtcgatg tccgaaggca cgcagaccac ccttggcgca tatcaccgta aatggcagtg
    12961 agaaaagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa agagacgctg
    13021 gaagaatttt atcagcaggc cgccgccagc agcgccgacg tgatttatct tggtgaagcg
    13081 gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcactc
    13141 gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc atcctccgaa
    13201 ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc
    13261 ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac
    13321 tgctacaacg cggtgacact gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg
    13381 ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggc
    13441 attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc
    13501 gcccgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc
    13561 attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtgctc
    13621 aatggcattc agaccatgag cggctacgtg tacaacctcg gtaacgagct ggcatccatg
    13681 caggggctgg tcgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcaatgctc
    13741 gatgccttcc gcgctaatga aaatggcgct gcgccactac cgttgacggc aaacagcgac
    13801 tgtaacggct actggcggcg gctggccggg ctggagctgc aagcgtaagt atatagctca
    13861 ctttgttaac aactttaact actctttaat gcagtattaa agattaatcg gtaacaaagt
    13921 gagctgttat gactgataaa accattgcgt tttcgctact cgatctggcc cccatccccg
    13981 aaggttcttc agcgcgggaa gcgttctccc actctctcga cctcgcccgt ctggctgaaa
    14041 agcgcggcta tcatcgctac tggctggcag aacaccacaa tatgaccggc attgccagtg
    14101 ctgcaacgtc ggtgttaatt ggctatctgg cggcgaatac caccacgctg catctggggt
    14161 ctggcggcgt gatgttgcct aaccactcac cgttggtgat cgccgaacaa ttcggcacgc
    14221 tcaatacgct ctatccgggg cgaatcgatt tggggctggg ccgcgcgccg ggtagcgatc
    14281 agcgaaccat gatggcgctg cgtcgtcata tgagcggcga tattgataat ttcccccgcg
    14341 atgtcgcgga actggtggac tggtttgacg cccgcgatcc caatccgcat gtgcgcccgg
    14401 taccaggcta tggcgagaaa atccccgtgt ggttgttagg atccagcctt tacagcgcgc
    14461 aactggcggc gcagcttggt ctgccgtttg cgtttgcctc acacttcgcg ccggatatgt
    14521 tgttccaggc gctgcatctt tatcgcagca acttcaaacc gtcggcacgg ttggagaaac
    14581 cgtatgcgat ggtgtgcatc aatattatcg ccgccgacag caaccgcgat gccgaattcc
    14641 tgtttacctc aatgcagcaa gcctttgtga aactgcgccg cggcgaaacc gggcaactgc
    14701 cgccaccgat tcaaaatatg gatcagttct ggtcgccgtc cgaacagtat ggtgtgcagc
    14761 aggcgctgag tatgtcgctg gtgggcgata aagcgaaagt gcgtcatggc ttgcagtcga
    14821 tcctgcgcga aaccgacgcc gatgagatta tggttaacgg gcagattttc gaccaccagg
    14881 cacgcctgca ttcgtttgag ctggcgatgg atgtgaagga agagttgttg gggtagtgtg
    14941 ttttaacgta ggaagcctta tccgagatgg caacgctgtc ctacgtaggc ctgataagcg
    15001 aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc tgattactga
    15061 tacaccggca gcacattaaa gctcgataag atatggacca gtgcgttgcc aacgccaaat
    15121 accagaatca gcataatcat cggcttgccg ccccatacac ggaatttcgg gctaccaaag
    15181 cgtttacgcg atgcgcgggc caacagcgcc ggaacaattg ccgcccagat ggtagccgct
    15241 aagccagcat aaccaatggc gtacaggaat ccgttcggga acagcaggcc acccacaaca
    15301 ggcggggcaa aggtcagcaa cgctgttttc aaacggccca tagccgagtc gtcaaaacca
    15361 aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga actcgctacc
    15421 gcaaagtttg agaacacgac cagtagaaga tccagactac ggctgttcag tacgccgctt
    15481 aacgcctgta ccagcacatc aatattaccg cccttctcgg cgataccgat aaactccgga
    15541 cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag cgccatcagc
    15601 gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata cttcatcagg
    15661 ctcggcacgt taccgtgata accaaaagat gccagacaga atggcagtgt catcagcaga
    15721 tacggcgcat aagacgcatt gctttcggcg acattgaaca aggtcgttgg ctgtacatgc
    15781 cccagcaggc tgccgaaggt gaggaagaaa gtaatgactt tcgcccccag cacaattgct
    15841 gtcatccggc tgacagcttt cgtactcaac cacaccacga acgctaccag cagtgcaaaa
    15901 ccaaatccag ctgcccgtgc agggacgttc agcgacatct ccgcgaaggt gtgatgcaga
    15961 atcgaaccgc tggcagaaat ataagcgtaa gtcaggatat agagcacaaa ggcaatggaa
    16021 atgccgttga ctacgttcca gcctttgccc agcaaatctt tggtgatggt gtcaaagcta
    16081 gaaccgattc gataattcag gttagcttcc agaatcatca agccggaatg cagcatacag
    16141 aaccaggtaa agatcagcgc cgccattgac cagaagaacc acgccccgga catgaccaca
    16201 ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac gccgccaagt
    16261 agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct ccagtgaaaa
    16321 atagtgcgac tgcgttgtta tgcattgcac tgtaccagta cacgagtaca aaagacagaa
    16381 aaaaagcccc gattgttaaa atcggggctg tatatattat tttacagatt gtgttcgctg
    16441 tttagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc atcacgacgc
    16501 ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg accaccttca
    16561 cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccaccgaa accacgacca
    16621 ccaccacgac gctcaccacc agtatgcggc tgtgcatcgc ccagcaactg catgttcatc
    16681 ggcttgttga gaatgcgagt acgcgtaaag tgttgcagca cttcacccgg catacctttc
    16741 ggcagttcga tggtggagtg agaagcaaac agcttgatgt taccgatgta acggctgcta
    16801 atgtcacctt cgttagcaat cgcgccaacg atatgacgaa cttcaacacc atcatcgcgg
    16861 cccacttcaa tacggtacag ctgcatatcg ccaacatcac gacgttcacg acgcggacga
    16921 tcttcacggt cgccacgcgg gccacggtcg ttacgatcgc gcggaccacg gtcatcacgg
    16981 tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag agtacgttca
    17041 ccctgtgcca ttttcagtag tgcggctgcc agagtttcga gatccagctc ttcaccttcg
    17101 gcagtcggct gaattttgct cagcagtgca cggtattgat ccagatcgct gctttccaac
    17161 tgctgctgta ctttagcggc aaatttttcc agacgacgtt tgcccagcag ttctgcgttc
    17221 ggcagttcta cttccggaat ggtcagcttc atggtgcgtt cgatgttgcg cagcagacga
    17281 cgctcacggt tctcaacgaa cagcagtgca cgaccagcac gacctgcacg accggtacga
    17341 ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac caccaggctg
    17401 atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat gtccagacga
    17461 ccatctttca ggcgttccag tgtctgctca cgcagcgcct ggttcatgtc accgttcagc
    17521 gcggcgctgt tgtagccgtt acgctcaaga gcttctgcca cttccagagt cgcgtttttg
    17581 gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg taccagtgct
    17641 tcgtttttac gcatacccca gacagtccag tagctctggc tgatgtcagg acgggtagtc
    17701 acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt aatgcgacga
    17761 atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg gatctgcgcc
    17821 ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc ttcatccaga
    17881 accagaccgc tcaatttaga gaggtccaga gtgccacgtt tcaggtggtc cagcagacgg
    17941 cccggagtac cgacaacaat ctgcggcccc tgacgcaggg cgcgtaattg cacgtcataa
    18001 cgctggccgc cgtacagggc aaccacgttt acgccgcgca tgtgtttaga gaaatccgtc
    18061 attgcctcag caacctgtac cgccagttcg cgggtcggtg ccaacaccag aatctgtggt
    18121 gctttcagct caggatcaag attctgcagc agaggtaaag agaatgctgc agtttttccg
    18181 ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg aatacactct
    18241 gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag gataggagcc
    18301 ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt gtgcctcaaa
    18361 attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt tcattgaaaa
    18421 gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc cgttaaggtg
    18481 atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg tccgattctg
    18541 ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat cggtgctcaa
    18601 caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg ctgtccaaat
    18661 cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga tgctcagcga
    18721 gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt tcgctaatgt
    18781 tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat ttttcgaagt
    18841 gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg agataaagcc
    18901 acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga tcatcttgcg
    18961 ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc gcgtagttgt
    19021 aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca aaattgcctg
    19081 cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt cggattgcca
    19141 gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca tacaacactc
    19201 cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg gcaaggatct
    19261 gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt accgcgagga
    19321 cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct gtcgcaacga
    19381 aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg aatgaacgtc
    19441 ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc gcagcggagg
    19501 gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct gcagcaggtt
    19561 gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga tcaacttcca
    19621 gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc tcaacgcgtt
    19681 tgtcagcgat ctgagagatg tggaccagac cttctttacc gccgccgatg gcaacaaatg
    19741 cgccaaagtc aacgatacgg gtcactttac cattgtagac gcggcccact tcgatttctg
    19801 cagtgatctc ttcgatacga cgaatcgcgt gtttcgcttt ctcgccgtcg gtcgctgcga
    19861 ttttcacagt accgtcatct tcgatttcga tggtggtgcc agtttcttcg gtcagagcac
    19921 ggataacaga accgccttta ccgataacgt ctttgatctt gtccgggttg atcttgatgg
    19981 tatggatacg cggagcgaac tcagagatat cgccgcgcgg cgcgttgatc gcctgttcca
    20041 ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagtgcaacc tgcatgatct
    20101 ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccg tcacgggaac
    20161 ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg tcagacagta
    20221 caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg gcagctttga
    20281 tcggcacacc tgcgtccatc agcgccagag acgcgccgca cacggaagcc atagaagagg
    20341 aaccgttgga ttcggtgatt tcagacacaa cacgtacggt gtacgggaat ttgtccatat
    20401 ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt tcacgacgct
    20461 tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg tagtggaaca
    20521 ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca cgagcggtac
    20581 ccaacgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca gaaccgtgag
    20641 tacgcggcag cacgccagta cgcacatcca gaccacggat catatctttt tcacgaccgt
    20701 cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac gtttttctca atagcgtgca
    20761 gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc gtcgcgatgg
    20821 tttcagattt gatgacatca acctgcgcat aacgctcttg tttatcggtg atgcggtaag
    20881 catcgctcag acgagcttca gccagcgcag caacgcgcgc gttcagcgct tcgtttaccg
    20941 gctccggctg ccaatcccaa cgcggtttac cggcttcttt caccagttca ttgatgttct
    21001 gaataacaac ctgctgttgt tcatgaccga acaccactgc gcccagcatt tggtcttcgc
    21061 tcagcagttc agcttcagat tcaaccatca gcacggcagc ttctgtaccg gcaacaacca
    21121 ggtccagttt gctctctttc agttcgtcct gagtcgggtt cagtacgtac tggtcattga
    21181 tgtaacctac acgggcagca ccaatcgggc cattgaacgg aataccagac agagacagtg
    21241 ctgcggaagc accaatcatc gcgacgatat ccgggttaac ctgcgggtta acagaaacca
    21301 cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg cgaatcgggc
    21361 ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca cgacggaaga
    21421 agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg gtcagcggga
    21481 agaagtcctg acctggtttg gcttttttct gaccaacaac ggtaacaaat accgcggtgt
    21541 catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg gtttccagag
    21601 tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat gtaatatcct
    21661 ttctctttct tagacagtac cttacggtac tggtgttaat acccgatctt ctgcgcatcc
    21721 tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca ttagccgcgc
    21781 gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag tgaattgctg
    21841 ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc aagaattagc
    21901 gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta cgtttcaggt
    21961 agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg ctgtggtgat
    22021 cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc agcagtgcta
    22081 cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa acgattttag
    22141 ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat gaaaggacgc
    22201 cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa tttacgcgac
    22261 gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta acattacgcc
    22321 gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat ttcgcccata
    22381 ccgataaact tgccgttctc accctctgta acgcgaacca gtccttccag cggcgcaccg
    22441 gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg aagattcacc
    22501 actgggtagt ccgaagctgg actgtccatt ggcatcagta atggatcaag taactccgca
    22561 gccggaatat cctgctgttc ggcttgctca accagttcac gcagatgttc cagggtcacc
    22621 atccgttcaa ccggatattt acttaccgcc agacgacgca agtaaataac atgcgcgcca
    22681 caaccgagtt tttcgccgag atcgtcaatg atagtgcgga tataagtgcc ttttgagcag
    22741 tgaatttcca gctccagctc attgccttca tggcgaataa acagcaattc ataaacggta
    22801 atcgggcgcg cttcacgcgg aacttcaatg ccctgacgcg cgtattcgta cagttttttg
    22861 ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc acggaaggta
    22921 tccagcgccg ccgccagttg ctctgcacta aaggttaccg gacgttcttc aacgatttgt
    22981 ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg atagcgtttg
    23041 tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat tggcaacatg
    23101 ccggtcgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt ataaatccgt
    23161 ttcacctttt gcagcgcatc gttgctggac atcccctgcg gtttatccag caacaaaacg
    23221 ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg ctgtcgtccg
    23281 ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt gacatgcgca
    23341 tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata cgcaggcgca
    23401 tcgctttccc cagcaggctg cggatgaaac cagaagcttc ctgcaacgct ttgatgcccg
    23461 ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg gcatacgcca
    23521 ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccagacga ggatctttaa
    23581 tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg cgctgcgggc
    23641 gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt agcccctttt
    23701 taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc gccagcgtcg
    23761 catcaggcaa tccatgcagg atgcggcata aacgccttat cccgcatgga accctaaaaa
    23821 ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac atcgccagtg
    23881 cggacatcgt tgtagttctt aacgccgata ccacattcca taccgttacg gacttcgtta
    23941 acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac cacgttgtca
    24001 cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat acaacctgcg
    24061 atggcaccaa atttcggtga tttgaacacg tcacgaactt ccgccagacc gataatctgc
    24121 tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc gatcaggtta
    24181 tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt acgtgcagag
    24241 gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc cagggtagcg
    24301 tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac ttcgtcagta
    24361 gacagtttca gcaaggagtc ggaaattgct tccacagaac cctgtacgtc tgccttcagg
    24421 acaatattca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt ctcgagttta
    24481 gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata gagtgcaact
    24541 tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc cggtacgccg
    24601 gacaggccaa ggatttccac cggaatggac ggacccgctt ccagcacttc ctgacccagt
    24661 tcgttacgca ttgcacgaac acgaccgtat tcgaagccac acagaacgat atcgcccttg
    24721 tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt atcgaggaag
    24781 gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc cagaacttcc
    24841 gcctgcagca ggatagcatc cagcagttca tcaataccgg tacccgcttt tgcagatacg
    24901 tgtacgaact ggctttcgcc gccccactct tctggcagga tgccgtactg ggagagttcg
    24961 ttcttaacgc gatccggatc ggcttctggt ttatcgattt tgttcacagc aaccaccacc
    25021 ggaacacccg ccgctttcgc gtgctggata gcttcgatgg tctgcggcat tacaccgtcg
    25081 tctgcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgtgc acgcattgaa
    25141 gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc agtttcaacg
    25201 tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggccac tttcgttgaa
    25261 cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat ggtcacaacc
    25321 ggcgcgcgcg gttcagccgc agcacccgtg tcacggtcgc tcattaccgc ctcttccagc
    25381 tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag ctgtgcggtt
    25441 tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat cgctttgatg
    25501 acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat ggtttcgccg
    25561 atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg cagcgaagaa
    25621 cctttacgtt ttccgccctt accgccacgt actgctgcgc gagcttcttc acgatcagct
    25681 tttgattcag cgtgtttgtt gcccttcttc ggacgcgctg ctttcgcgtt acgaccacgg
    25741 ccacggccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc atgttgagaa
    25801 gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt ccatttgttt
    25861 tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc gagtttacga
    25921 cgcgcttctt cttcggcttt acgcttgagc tctgcagctt cctgctcacg gcgggctttt
    25981 tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc tttttccgca
    26041 gcttcacgtt tcgcttgttc agcagcttca cgcttagcct gttctgcggc ctcacgttca
    26101 gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc ttgctcttcc
    26161 gcttcacgct gcgcttgctc ttccgctgca aggcgctcag cctcttgcgg atcgcgtttc
    26221 acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc accggtacca
    26281 ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg gcctgaattt
    26341 ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga gtcgtcagca
    26401 gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc
    26461 tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc tacagtttat
    26521 tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc tccggctttt
    26581 tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc gagatcttcc
    26641 agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctac cccttcaagg
    26701 ttcagcagat cgtcagccgg tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg
    26761 gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct
    26821 tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt cgagaagcct
    26881 tcttctacca gaacagtcgc gaagtcttca tcgatgtcga gatatttggt gaaggtgtcg
    26941 atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt catcacgttg
    27001 agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccgttacg gccaatcgcc
    27061 tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc atccaccacg
    27121 atagaagcaa cgtctgctgg tgccattgcg ttaatcacga actgcgccgg gttatcatcc
    27181 cacaggacaa tatcgatacg ctctccgccc agttcagtag aaaccgcctg aacacgcgcg
    27241 ccacgcatac ctacgcaagc acctaccgga tcgatacgtt tatcgttggt tttcaccgca
    27301 attttcgcac gagatcccgg gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg
    27361 atttccggca cttcaatacg gaacagttcg atcagcattt ccggcttgga gcgggtgacg
    27421 aacagctgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg aacgcggtca
    27481 ccagggcgga agttttcacg cggcagcata tcttcgcgca ggatcacggc ttcagcgttg
    27541 ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt
    27601 tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc acgcactttc
    27661 tgcacgataa cctgttttgc cgtctgggta gtgatacggt caaaggtaac agactcaatc
    27721 tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt cataacgtgc ggcttcaagg
    27781 gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg gaaggtgtca
    27841 aaatcaccgc ttttgcgatc gatctgtacg cgaacgtcga tttcttgttc atattttttc
    27901 tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc
    27961 ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc gggcttttca
    28021 cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt actcagcgcg
    28081 aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac cgctttgata
    28141 acgccctgcc atttacgacg gttttgtacc gccatacgga gaaccagagt cacctcttct
    28201 ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc cggtgaggag
    28261 acttccaggt tataagcaac ggtgatgggg tcttcaacat ccagcacagc acttacctgg
    28321 tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat atagatgcgc
    28381 agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc cagggcctca
    28441 actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc cacccccaag
    28501 acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa aaccccgata
    28561 aatcggggct ttatataact gaaccctata accgcaactg cggtctggag cactttccag
    28621 aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga
    28681 ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg gttatgagcc
    28741 cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt ggcaaattct actcgttttg
    28801 ggtaaaaaat gcaaatactg ctgggatttg gtgtaccgag acgggacgta aaatctgcag
    28861 gcattatagt gatccacgcc acattttgtc aacgtttatt gctaatcacg tgaatgaata
    28921 tccagttcac tttcatttat tgaatacttt taccttctcc tgctttccct taatcgcatt
    28981 attttacaaa aaacacacta aactcttcct gtctccgata aaagatgatt aaatgaaaac
    29041 tcatttattt tgcataaaaa ttcagtgaga gcagaaatcc aggctcatca tcagttaatt
    29101 aagcagggtg ttattttatg acgacgattc tcaagcatct cccggtaggt caacgtattg
    29161 gtatcgcttt ttccggcggt ctggacacca gtgccgcact gctgtggatg cgacaaaagg
    29221 gagcggttcc ttatgcatat actgcaaacc tgggccagcc agacgaagag gattatgatg
    29281 cgatccctcg tcgtgccatg gaatacggcg cggagaacgc acgtctgatc gactgccgca
    29341 aacaactggt ggccgaaggt attgccgcta ttcagtgtgg cgcatttcat aacaccactg
    29401 gtggactgac ctatttcaac acgacgccgc tgggccgcgc cgtgaccggc accatgctgg
    29461 ttgctgcgat gaaagaagat ggcgtgaata tctggggtga cggcagcacc tataaaggaa
    29521 acgatatcga acgtttctac cgttacggtc tgctgaccaa tgctgaactg cagatttaca
    29581 aaccgtggct tgatactgac tttattgatg aactgggtgg ccgtcatgag atgtctgaat
    29641 ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga aaaagcttac tccacggact
    29701 ccaacatgct tggtgcaacg catgaagcga aggatctgga atacctcaac tccagcgtca
    29761 aaatcgtcaa cccaattatg ggcgtgaagt tttgggatga gagcgtgaaa atcccggcag
    29821 aagaagtcac agtacgcttt gagcaaggtc atccggtggc gctgaacggt aaaaccttta
    29881 gcgacgacgt agaaatgatg ctggaagcta accgcatcgg cggtcgtcac ggcctgggca
    29941 tgagcgacca gattgaaaac cgtatcatcg aagcaaaaag ccgcggcatt tatgaagccc
    30001 ctgggatggc actgctgcac attgcgtatg aacgtttgtt gaccggtatt cacaacgaag
    30061 acaccattga gcagtatcac gcgcatggtc gtcagttggg ccgtctgctg taccagggac
    30121 gttggtttga ttcccaggcg ctgatgctgc gggactctct gcaacgctgg gttgccagcc
    30181 agatcactgg tgaagttact ctggagctgc gccgcgggaa cgattattca atcctgaata
    30241 ccgtctcaga gaacctgacc tacaaaccag agcgtctgac gatggaaaaa ggcgactcgg
    30301 tgttctcgcc agatgatcgt atcggtcaac tgaccatgcg taacctggat atcaccgata
    30361 cccgcgagaa acttttcggt tatgccaaaa ctggcctgct ctcctcctct gccgcttcag
    30421 gcgtgccgca ggtggagaat ctggaaaata aaggccagta attcgacatc aaccctgccc
    30481 ttcaggggtg gggttacctt gattatcctc tgtaaactcc tttctgagca tcacataatc
    30541 gaaaacatca tggtttgcat ccacaacccg tgagtctcct ttatagtgtg caatcacttc
    30601 ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc cacgcattaa taaggtaatt
    30661 attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga tccacgccat ccccaagaac
    30721 ggggctatac cagataaaca tcgggacatg atatgcctgc tggctagcct ccctgcctcc
    30781 atgaaaatag acgttcttct tcgtagggtc gcgttccagg ccgtggtcgg caaaatacat
    30841 gaccgaggcg cggcgatctt ttaataattc aaaaacctga cctagcaaac tatctgtgta
    30901 atgaatggag ttgtcatagc aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga
    30961 ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa caatcagttt
    31021 cttttgctgc gtcttttgct gtaacgcttg cgataaatgc ggcaacaaca attcatcaaa
    31081 tcctctgaca tagactgttt ccatggcgcg catggcgata ctggtaactg ctgtaccatt
    31141 ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt gattagccat
    31201 attaataatg ttgtccgggt aattatgaat gtcatgactc aaaacagaat cagcagttaa
    31261 agaaagggga accgacagcg cagtgtaagg tgcaccgctt attgcttgat taaacagttt
    31321 gatctgtttt ctttgtgctt cgacttgcgg tgtcgtagag cgtgtatatc catacaaaga
    31381 catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa tacctgtatc
    31441 cctgactgat agttgaaaat acggcaccgt gtttgcaatt gagagtaatc tttgatgctc
    31501 tttcgctgct aaagcaaaat aattgagatt gaaaaacggc gtataggtag caaatcgcga
    31561 cgctagtata tatggactga acgcctcttt attttttgca tctttataag caaattgaca
    31621 agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca ctttttttgt
    31681 cggcaaggaa acatcatatt ttattattac tgccaaaaaa agaagggata aaaaggtaaa
    31741 agcacataga taagggatat acattcccag cattttgaca acttcatccg gatcactctg
    31801 cagcacacta atcgcaaatc catcattgaa agttgtacca aagatacacc atgaataaag
    31861 gctaatactc atatctgccg cagtcagtaa cacaaatggt acggcaacaa gcaggcgaag
    31921 cggaagggat gatatacgtt taacaaccag gatggtcaga acgaaaaaca gcaatcgctg
    31981 aaccatatgc gctccggaag aggcgactaa gttcgtaata ccaaaaacaa taacacaaag
    32041 atacaacaac caatgagatt taaaactttt aacaaatttg ttgaatactg tcatccggaa
    32101 atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat atcagacatg
    32161 tttttcaatt ctgatccata tcagaccgaa acactgttag attttttctt tttaaatgat
    32221 gcggccattg cgttctcagt aattaaataa cattatcaat gcgtctctaa acattacgca
    32281 gaacgtagcc atcagtagtt atactgacag gggcaggatg gtttatcgag aagtaaacat
    32341 atccgtattc gtaccggaaa tggagttgac acaataaagt gccaattatg tcagcagaag
    32401 ggaaaagctt ataactaagg cataccggca ccatcgtttc aaggtaccag ctacgagtaa
    32461 aacaactgga cgagatacag atacctgaca accattcctc agaccaggac caaaacgaaa
    32521 aaagacgctt ttcagcgtct ttctggaatt tggtaccgag gacgggactt gaacccgtaa
    32581 gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca cctcggcacg
    32641 gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagctgc tggctgagtt
    32701 tgttcggttt tcgccggcgc actcaggttt tcccattcgc tacctttatt ggttttgttg
    32761 ctattgatgt tacccagcac cagactgatg atgaagaata acgttgccag cagcgctgtc
    32821 atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga agcgcctgct
    32881 ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag accaacaagg
    32941 ccaattgcca caataaggaa aactactaaa agagcttcgt acataatcaa cctgttcctt
    33001 gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa tctttcccac
    33061 tgaagcgggt gtgaatacta accaaagcga atgaccttcg caagggcaat tttattgcat
    33121 tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa aaggcgacaa
    33181 tcgccgcctt tttagccagc catttatcgt tttaaacggc ttttactgca tcggcgatgc
    33241 ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc caccatcacg cgaattaacg
    33301 gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc gcttcaactt
    33361 ctgcggtcac agctttgact gactcatgct ccagcggatc gccgctgcct gctgtgtaac
    33421 gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg tgcaggctca
    33481 tatgattacg cgccatcgcc gccagcacct gcaaaccagc aacgatgccg tcaccggtag
    33541 tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc cagcctttct
    33601 cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat ggaattccta
    33661 actgtttcag cgccagttca agccccatgt tgctcatcaa tgtacccaca gcgccaccac
    33721 gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga tcgccatcga
    33781 ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg aaggcaatac
    33841 cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg gtagccccca
    33901 cttcggcatt gatatttaca ccgttcggct cacaaccgat agcgataacg ttcgccccca
    33961 gttcacgcag cacgttcggt gcgatgtgat aagtcgcacc gtttgcacaa tccaccacaa
    34021 tcttcagttc actgaggcta agttcgttcg ggaacgtggc tttgcaaaac tcgatataac
    34081 gacccgcggc atcaacgata cggctggctt tacccagttc tgccgaatca acacagctga
    34141 tctctttttc catttccgct tcgatggctt cttcaactgc atccggcagc ttagtaccgt
    34201 caatagaaaa gaatttaatg ccgttatcgt agaacgggtt atgcgatgca gaaatcacaa
    34261 tgccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt ggcatcggac
    34321 cagtgaagag tgcggaaagg ccggctgccg caagccccgc ttccagtgct gactccagca
    34381 tatagccaga aatacgcgtg tctttaccaa tgatgatctt acgggagccg tggcgcgcca
    34441 gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggggtgatc ggcgcatccc
    34501 ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata gcgtttgttt
    34561 tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc tttgacgtca
    34621 tgaacacgaa tgatgtgcgc gccttgcatt gcggcaatga ccgcacaggc cagactaccg
    34681 ctcaaacgct cggacggccc cacgttcagc agctgtccaa tcatcgattt tcgtgacata
    34741 cccaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag taatgaatag
    34801 ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt ctcttttgcg
    34861 atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac ttctgcaaag
    34921 acatcgtgat acttcggggc ttcctgcatg gtttttggat ttccctgcat atgcatcaga
    34981 caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga aagggagcgg
    35041 atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc tggtttggat
    35101 gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac aggaataaca
    35161 cgttgtaact cttcttcaac gctaacttcc gccgcccctg gacgtgtgga ctcgccacca
    35221 acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac cgcatctatc
    35281 agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag gatccccatt
    35341 acgtgaggat ggctaaggtc cagtgaagta ccctgggcaa agagtttcat aatgttatcc
    35401 ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggtttttg gtacaaatac
    35461 agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg ttacccgggt
    35521 tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg tcgccagaat
    35581 tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga cgtgccatca
    35641 ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct ttcatcgcat
    35701 gcagtatatc catattgtcg gtcagaagct gacgcgcacg attatagtta cgctcaatca
    35761 gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt ttcgctttcg
    35821 ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt ggacccaatt
    35881 tctcagagaa gccccactga gtcaccatgt tacgtgccag gttggtcgca actttaatat
    35941 cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct gccagacgac
    36001 caccgtacag cgtagaaatc tggctttcca gtttctgacg gctagcgctg attgcgtcgc
    36061 cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc gtcactttgt
    36121 gcaccggatc atgttccggc accaggcgac cgataatcgc atgacccgct tcgtggtaag
    36181 ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca cccatcatga
    36241 ttttgtcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg ccacgagcag
    36301 cgaacagtgc cgcttcgttc accaggttcg ccaggtcagc accggagaaa ccaggagtac
    36361 cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc atgtgaactt
    36421 tcaggatctg ttcacgaccg cgaacatctg gcaagccaac cacaacctga cggtcgaaac
    36481 ggccaggacg cagcagcgcc gggtcgagaa cgtccggacg gttagtcgcg gcgataacga
    36541 tgataccttc attaccttcg aagccatcca tctcaaccag catctggttc agagtctgtt
    36601 cacgttcatc gtgaccaccg cccagacctg cgccacgctg gcggcctacg gcgtcgattt
    36661 catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg tcacgaacac
    36721 gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata gtaaagaacg
    36781 gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta cccggaggac
    36841 cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg ctcggctcgc
    36901 gcagatactc aaccagttca gcaacttctt cttttgcttc gtcgcagccc gcaacgtcag
    36961 caaaggtcgt tttgatctga tcttcagtca gcatgcgcgc tttgctctta ccaaacgaca
    37021 tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccag acaccaatca
    37081 gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt tcttcaggcg
    37141 gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc ggatcctgaa
    37201 ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt tcacgtccgt
    37261 tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta gagtaatcca
    37321 ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc agcacaacgg
    37381 caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta acctcttatt
    37441 acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca aactttcgtc
    37501 tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc cgcgaacgtg
    37561 cgcgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga atttccctta
    37621 gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca cctggcgcta
    37681 atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg gggatatcca
    37741 ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg actttgctgt
    37801 cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg ccctgaagaa
    37861 agtccacacc aacgatagga tccataggta aaagatcgca agcgatgatg cggcctttgc
    37921 cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca aggtcgacaa
    37981 ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt ttaaaccagg
    38041 cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta aagtgttcct
    38101 gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt tcccattggg
    38161 ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggcgat atatgggaga
    38221 tggcggtaga atgacccgtt ttcaatccca acgtaagcaa aaaatacgat gaatctgagt
    38281 actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt tgttctgctt
    38341 ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt agagcaccat
    38401 gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa ctaaaacctt gatcgtggaa
    38461 gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac gctggtgctt
    38521 tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc taaagttaca
    38581 cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat gccacgctct
    38641 gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac atcaacatct
    38701 tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc tacttcgccg
    38761 cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc acgagcaata
    38821 ggcgagttta cagaaatcag gttttgttta aagtctgctt cgtcatcgcc tacgatgcga
    38881 taggtctgtt cttcatcaga atccagattc aaaaccgtca cggtagcgcc aaaaatcacg
    38941 cgaccattgt tgggcatttt ggtgacatca attacctgcg cgttcgacag cttggcttcg
    39001 atatctttga tacggccttc gcaaaaaccc tgctgctcac gagctgcgtg gtattcggcg
    39061 ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat ttcaggacgg
    39121 cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg taaggtcatc
    39181 ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt cttcacccac
    39241 cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa aaaaataccg
    39301 acccgggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt ttaccctgga
    39361 gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga cggctcgatt
    39421 ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt gaccagctgt
    39481 atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact ccccgcaggt
    39541 gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat tgattaccac
    39601 agtcagcaga tggcgctacc cgccagtacg cagaaagtga ttactgcgct ggcggcgttg
    39661 attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg caatgtggaa
    39721 aacggcatac ttaagggtga cttagtagcg cgatttggtg ccgatccgac gttaaaacgt
    39781 caggatattc gcaatatggt cacgactttg aaaaaatctg gcgtcaacca aatcgatggc
    39841 aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg ctggccatgg
    39901 aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga ccgcaactgt
    39961 ttctccatct cgctctacag tgccccaaaa cctggtgata tggcttttat acgcgtggca
    40021 tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg ttctgccgaa
    40081 gcgcaatact gcgaactgga tgtcgtacct ggtgacctga accgctttac gctaacggga
    40141 tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga tggtgccagc
    40201 tatgccgggg caattctgaa agatgagtta aaacaggcgg gtatcacctg gagcggaacg
    40261 ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa acagtcggcc
    40321 ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg acaacatgat tgccgatacg
    40381 gttttccgca tgatcggcca tgcgcgcttc aatgtgcctg gaacatggcg ggccggatcg
    40441 gacgccgtgc gtcagatcct gcgccagcaa gccggtgtcg atatcggaaa caccattatt
    40501 gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat gatgcaggtg
    40561 ctgcaatata ttgcccaaca cgacaatgaa cttaacttta tctccatgct gccgctggcg
    40621 ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt ggatggaaaa
    40681 gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt cattaccaca
    40741 gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt agaaccggcg
    40801 gatcagcgta atcgccgtat tccgttggta cgttttgaaa gccgtttgta taaagatatt
    40861 taccagaaca attagtctaa agagaaaccc cggcacatgg ccggggtttt gctgattaac
    40921 gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtctt
    40981 catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc cacatgaatt
    41041 cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg
    41101 tcatcacatc ccagcagaga tctttcacgc ccagtccgct cgccgcagag atcagataat
    41161 atttatcttc ccaacccagc gcctcagcga tcgctttcgc tttctcttcg gcttctgcct
    41221 tatccagcaa gtcgatcttg ttgaagacta accaacgcgg tttcgccgcc agatcctggc
    41281 tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccatcaa
    41341 tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ctccaggtgc ttcaggaagc
    41401 gaatgcccag acctgcgcct tctgcagcgc cttcaatcag tcctggaata tcggcaacaa
    41461 cgaagctctt ttcgttgtcc atacgcacca cgcccaggct tggtaccaga gtggtaaacg
    41521 gataatccgc cactttcggt ttagccgccg ataccgcacg aataaaggtc gatttacccg
    41581 cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagt agcagctcac
    41641 gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact gacgatttga
    41701 aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt
    41761 tggtcatatc gcccatggtt tcaccggtac cctggtcgat aacacgcgta cctacaggca
    41821 ctttaatcgt cacgtcttta ccgcgcttac cggtacagtc gcggcttgcg ccattctgac
    41881 cacgctctgc gcggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttctcgt
    41941 cggcttccat ccatacgtca ccgccgtcac cgccgtcgcc gccatccggg ccgcctttcg
    42001 gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca
    42061 gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg
    42121 gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg
    42181 aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg
    42241 gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca
    42301 gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg
    42361 ccagggcgcc atatcctacc aatgtattca gtccgcaaaa aattaaacat gcgagctgcc
    42421 agtggctaag ctgcgctatc actccaggat ttgccagagg gaagagcgca attgtacata
    42481 aagtgtacaa caaaaacagg atctgcggtg aagccagccg acgcaataaa accttttgcg
    42541 ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt
    42601 aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc
    42661 tcaggagcat caatgcccct acaacctgag tactgcgcat tttctctttc aggataaata
    42721 cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac
    42781 tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca
    42841 ctgctaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc aaccgcttct
    42901 tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg
    42961 tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa
    43021 ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc
    43081 tgcaaagcgc atatgcggat taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt
    43141 aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt
    43201 cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac
    43261 cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga
    43321 atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac
    43381 gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct
    43441 ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac
    43501 atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt
    43561 aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc
    43621 atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc
    43681 gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc
    43741 gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc
    43801 cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata
    43861 ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca
    43921 acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcacccta tattcctaac
    43981 cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt
    44041 gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg
    44101 gcaatccttg agcagcttaa ttccgacgtc caactgatca atcagttagg ctattacatc
    44161 gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt
    44221 ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat ccacacggcg
    44281 actctgctac acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc
    44341 aacgccgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg
    44401 ttccagatga tgaccagcct cggttcactc aaagtgctgg aagtcatgtc agaagccgta
    44461 aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact
    44521 gaagaaaact acatgcgcgt tatctacagc aagactgcgc gtctgtttga ggctgccgcg
    44581 cagtgttccg ggattctggc tggctgtacg ccggaggagg aaaaaggcct acaggattat
    44641 gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat
    44701 ggcgaacagt taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc gacgctgccg
    44761 ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgattcg taccgccatc
    44821 gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga
    44881 tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag acaaagccat cgcagcgtta
    44941 caggtgctcc cggacacccc gtggagagaa gcacttatcg gcctcgcgca catcgctgtt
    45001 caacgcgatc gttaatcccc tcccctcatc ccgcgcagtg cgcgggatga ttccagtaaa
    45061 ttccataaaa acacttattc agctctataa atcctgaaaa acagggctgt gtaatcttta
    45121 tatattctga atattcacgc tctttacagg aactttttag agcaataggc catcaggagt
    45181 atagtgatgc tcgacagaaa cagaagaagt gttctgaatg aaagcgacta acttaaggag
    45241 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcaggtttg
    45301 cgcaagaagg gaacgtcaat ggcggcggag tctcgcagaa atggtttgag ttcctcaacg
    45361 ctggcgaacg cattatcacg cccatggccg aaaggagaga tgattattgc gaaagccctg
    45421 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    45481 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    45541 tagccccgcg aacggggctg ccagctctca gacaattact cgcctttcac acgctcaata
    45601 tttgcaccta aagcgcgtag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    45661 atacgatcaa ccaccgtcgt cccttccgca atacagccag ccagcaccag gcttgctgat
    45721 gcacgcagat cggttgccat aacctgtgcg ccagaaagtt tttcaacacc gtgacaaata
    45781 acggtattgc tttcgatttc ggcgtgcgcg cccatacgac tcagctctgg cacatgcata
    45841 aagcggtttt caaagaccgt ttcggtgatg aatccggtcc cttctgccac cagattcaac
    45901 agcgtgaact gggcctgcat atcggtaggg aatgccggat gcggcgcggt acgtacgtta
    45961 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    46021 tccgctccag cgtcacgcag tttcgccagc acggcgtcca gagtatctgg ctgcgcgtta
    46081 cggcagataa ttttgccgcg agagatcgcc gccgccacca ggaaagtacc ggtttcgata
    46141 cgatccggca gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    46201 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    46261 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg tgccttccgc aagggttgca
    46321 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    46381 ttcaaacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    46441 aattgttcga ggccagaaat gtgtagatca accggacgtg cgccgatcgt acaaccgcca
    46501 ggcagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagcgc ccagatagaa
    46561 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    46621 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag cttcattgat
    46681 gtatcgacgt cttttagttt cgggacgttc tggatctcta ccggttcttc cgccagtagt
    46741 gcggcaaaaa ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    46801 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    46861 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    46921 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    46981 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac
    47041 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    47101 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    47161 agttaataaa tcagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    47221 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    47281 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    47341 ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg tcacattgtt gccctgcttt
    47401 ttcgccagat cgataagatg gagcagcagt gccagtccac ccgtatccac gcgagacaca
    47461 cggctaagat cgatgctggt aatccccttc accgcttcct cacgtatttc ccaaagcggt
    47521 agcagaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    47581 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    47641 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    47701 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcctgc cagttgcccg
    47761 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cgggcggcca ttcgggtcaa
    47821 taatggtaac gcgaatcggc acaatggttt tatcacccag cggctgttct ggcgcaatct
    47881 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    47941 ggaaagcggc aaagtaagca tcacgttgag ctggggtcgc actcttgtaa tactggccca
    48001 gcaccagcgc accggcgtat ttcacctgta cgtatggcaa cagttcctga tcaacaatgg
    48061 tacgcagata atccgggtta gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    48121 ttttctgcgc cgcctcgtcc atcagcttat acggattggt ctggtctgcc gcggttgccg
    48181 cactcagcgg tgcaatcacc agcaaagcaa ccatcattaa acgtttaaac atgcgtcggt
    48241 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    48301 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    48361 caatgagatc ttccagcacc atcgcagact tggtgtcctg aattgtatcg ccatccttca
    48421 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc
    48481 ccagcaggcc ggaagtacga atgctcagcg aactggtatc aggaatgtgg ttataacgtt
    48541 gttcaatttc cagcgttacg cgcggcagat atgttttcgg gtccagcgta atatccgcca
    48601 cccggcccac aacaacacca ccaatgctga ccggagagcg cgctttcagg ccgccaatgt
    48661 tatcgaacgt cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg
    48721 ccttcaggca aacaaacagc gccgccagta gtgctgctaa taaaaagata cccacccaaa
    48781 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    48841 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    48901 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    48961 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    49021 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagcgat
    49081 ccgccccaga tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca
    49141 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    49201 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc
    49261 ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    49321 agcataccca gactggtttc cgcactataa gtggtcagaa ccagataacc ttgcagcccc
    49381 aacaccattc cgatgaacac gccagaaact acaataatca gcatcgacag gacgccgaca
    49441 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    49501 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    49561 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    49621 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    49681 ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat gagcgacaat
    49741 ttttttgtcc gccaggatcc aggcgtgatc ggcaatactt aacacttccg gcacatcgtg
    49801 agaaaccacc acacaagtca cacccagcgc gctgttcagc tcagaaatca gcttcaccag
    49861 tacgcccatg gtgatgggat cttgcccaac aaagggttca tcaaacatga tgagatccgg
    49921 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    49981 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    50041 atgcaacaat ggcgcgggaa gttgagtatg ttcgcgcagt ggataggcga cgttgtcaaa
    50101 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac
    50161 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa gaatctcacc
    50221 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    50281 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    50341 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    50401 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    50461 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    50521 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    50581 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    50641 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    50701 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    50761 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    50821 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    50881 cgctggttcg tccttttacc gtccattctg atgttctacg ccgtgaatta cccttaatgt
    50941 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgcagcgatg
    51001 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    51061 aggctgaacg tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg
    51121 aaggtggatt gcccgtcgca tttttatggc tcggcatcgc actgatcatt atgccagtgg
    51181 ccacgcggat ggtagttgat aacgccacgg tgctggcgaa ttactttgcc attagcgagt
    51241 tgacgatggg tctgacggca attgctatcg gaaccagcct gccggaacta gcaaccgcaa
    51301 tagcgggggt tcgcaaaggt gaaaacgaca ttgcggtcgg aaatatcatt ggcgcaaaca
    51361 tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga gagattgatc
    51421 cactggcgta cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc
    51481 tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg ggtattatta actggcggat
    51541 ttatcgtatg gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa
    51601 cgcattatgt cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa
    51661 gtcctggcga ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc
    51721 acgcttgcct gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg
    51781 aaatcggggc acatcgggcg caaaatggca gcaacatttg ccagcaccgg tacaccttca
    51841 tttttcgtcc atcctggcga agccgcgcat ggtgatttag gcatggtcac cccacaggat
    51901 gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg
    51961 cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg
    52021 cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg
    52081 gcaccgacca gcagcacaac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg
    52141 ttaaaagcac gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg
    52201 ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat
    52261 gttaagaaaa cggccagtct gcgtgacgca ttgctggaag ttacccgcaa aaatcttggt
    52321 atgactgtca tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta
    52381 cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg
    52441 ccggggggaa tacgcgtgcg cccaggcatt ctggccgttg aggcactgaa cttaatgcag
    52501 tcccgccata tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat
    52561 atgcatgatt tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca
    52621 aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atggcaaaag
    52681 cagagaacat tcgtctgctg atcctcgacg ttgatggcgt actgtcagat ggcctgattt
    52741 atatgggcaa taacggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc
    52801 gttgtgcgct cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg
    52861 tagaagatcg ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaacaaac
    52921 tgatcgcctt tagcgatctg ctggaaaaac tggcgattgc cccggaaaat gtggcttatg
    52981 tcggcgatga tctcatcgac tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg
    53041 ccgatgcgca tccactgttg atcccgcgcg ccgattacgt aacgcgcatt gctggcggtc
    53101 gtggcgcagt gcgagaagtt tgcgacttat tactcctggc gcagggcaaa ctggatgaag
    53161 ccaaagggca atcgatatga gtaaagccag acgttgggtt atcattgtgc tatcactggg
    53221 ggttctggtg atgatcggca ttaatatggc cgaaaaagac gataccgccc aagtggtcgt
    53281 caacaacaat gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga
    53341 aggggcacta agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt
    53401 ttcgtggttt acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc
    53461 cgtaaaagca gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt
    53521 tgaagtcaat gcactcgtgc cggactctca acttcgcaga atcacgacgg ataacgcgca
    53581 gatcaatctg gtgacgcagg atgttacctc tgaagacctc gtcacgttat acggaacaac
    53641 atttaactcc agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat
    53701 tgaaaaggtt agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc
    53761 ttgccagctc acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc
    53821 agccgatcca cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct
    53881 ttaccggcaa tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg
    53941 ttacccgtcc gggcggcgaa caaggtaaag aagtgattga cggctacggt aaaccggcaa
    54001 cgttctacca gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc cagatgcact
    54061 acgaactggc aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata
    54121 gcaacattaa gggcgataag atcacttacc tggtgaaaga gcagaaaatg caggctttca
    54181 gcgacaaagg caagcgcgta accaccgttc tggtgccgtc gcagctgcag gacaaaaaca
    54241 ataaaggcca gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg
    54301 caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg
    54361 tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt
    54421 tctacatggt tgttggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg
    54481 atatcagtct gctgcctctg catgcacgcg cgcgccgcgg tatcggctat ctgccgcagg
    54541 aagcctccat tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc
    54601 gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc
    54661 acattgagca cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg
    54721 tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg
    54781 ccggggttga cccgatctcg gttatcgaca ttaaacgcat cattgagcac ctgcgcgaca
    54841 gcggcctggg cgtgctgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac
    54901 gcgcttatat cgtcagtcag gggcatttga tcgcccacgg cacgcctaca gaaatcttac
    54961 aagacgaaca cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa
    55021 gtttgcgacg ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag
    55081 gcttagccaa caactggcga tgacgccaca gctccaacag gcaattcgtc tgttgcagtt
    55141 gtcgacgctg gaacttcagc aggagctaca gcaggcgctg gagagcaatc cgctgcttga
    55201 gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg caagacagtg aaacactgga
    55261 caccgccgac gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg
    55321 ggacaccatt tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgacga
    55381 gctgccggtc tatcagggcg aaacgacaca gaccttacag gattacctga tgtggcaggt
    55441 cgagttgaca ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt
    55501 tgatgacacc ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga
    55561 agagattgac atcgacgaag ttgaagccgt ccttaagcgg atccaacggt ttgatccggt
    55621 cggtgtggcg gcaaaagatc tgcgtgactg cctgctgatc caactctccc aattcgataa
    55681 gaccacgcca tggctggaag aggccagact gatcattagc gatcatctcg atctattagc
    55741 caatcacgac ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga
    55801 agccgtcaat ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga
    55861 acctgagtat gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacggtaga
    55921 actcaacagt gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa
    55981 taacgcgcgc aacgatggtg atagccagtt tatccgcagc aatctgcagg atgccaaatg
    56041 gttgatcaag agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt
    56101 tgaacagcag caagccttct ttgagcaagg cgaagaatat atgaaaccga tggtactggc
    56161 tgatatcgcc caggccgtcg agatgcatga atcgacgata tctcgcgtga ccacgcaaaa
    56221 atacctgcat agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa
    56281 tactgaaggc gggggtgaag cctcttccac agcgattcgc gcactggtga agaaattaat
    56341 cgcggcggaa aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga
    56401 acaaggtatc atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc
    56461 gccgtcaaac cagcgtaaac agctcgtttg acccaaccga taaggaagac actatgcagc
    56521 tcaacattac cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttacagcca
    56581 aatttgccaa acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag
    56641 tggagaaagt cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg
    56701 ccagcgcgga aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac
    56761 gccagttgac caaacataaa gataaactga aacaacacta attgtccggg caattagcat
    56821 gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct
    56881 gttccactgt ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc
    56941 tacagcttag cagtgttctt aacagggaat gtacgcgaag ccgcgtccac tgtcagagca
    57001 aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctggcgcctc
    57061 aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg
    57121 gtattgccat tccgcatggc aaactggaag aagatactct gcgtgccgtt ggcgttttcg
    57181 ttcagctcga aacgcctata gctttcgatg ccatcgacaa ccaaccggta gacttacttt
    57241 ttgccctgct ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtag
    57301 cgaaacgtct ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag
    57361 agctgtatca aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta
    57421 atgtctcttt tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac
    57481 gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg
    57541 tggataacct tcccgtagtg ttgttacccg atctggctcg aactctggcc gatcgagaga
    57601 tttctgccgc cgtcagcatt gatgttcgta atatgccgga gtcaccagaa atattcgaac
    57661 aggcgatgag taacctgcct gacgctttct caccgcaact actgttcctg gatgccgacc
    57721 gtaatacctt aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa
    57781 acctgtcgct ggaaagtgct atcgacaaag aaagcgattt gctggagcct ctgcgttcgc
    57841 gagcggatct gattgtcgat acctcagaaa tgtccgttca cgagctggca gaaatgctgc
    57901 gtacccgtct gctgggtaaa cgtgaacgtg aactgaccat ggtctttgag tctttcggct
    57961 tcaaacacgg tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc
    58021 cgcactggga tccgaaactg cgtccaatga caggtcttga taaacctgtc gccgcgttcc
    58081 tcgaccgcca cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat
    58141 ggttacctat gctggaaacc aacaaccgta gctacctgac ggtcgccatt ggttgtaccg
    58201 gcgggaagca ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg
    58261 gtaaaaacgt ccagtcacgc catcggacgc tggaaaaacg taaaccatga ccgtcaagca
    58321 aactgttgaa atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga
    58381 attaatgcag ggttttgacg ctgaagtgct gttacgtaat gacgaaggca ccgaggctga
    58441 agccaacagc gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt
    58501 tgaagcgacc ggtccacagg aagaggaagc actggccgcc gttatcgccc tctttaactc
    58561 tggttttgat gaagattaat cttcatcact ttgacattca aatcccttca agctcccccg
    58621 gcaataagaa aagcgcgtac tgaaatcgtt atcaatcaac gtcgattgta catttcgtgc
    58681 gctttttact ttcaaaagaa ccttaagaaa accttaagaa gcattgttta gatttcgttt
    58741 aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg catgattcgc
    58801 ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc gcatccggaa
    58861 gatgcccaac gctgtattaa gattgtctac catcgtggcg atggtggcga taaagagatc
    58921 cgccgtgagt taaagtacta cgcgcatctt ggtcgccggt taaaagactg gagtggaata
    58981 ccgcgctatc acggtaccgt tgagacggat tgcggaaccg gttatgtcta cgatgtgatt
    59041 gccgatttcg acggcaagcc ttccattacg ctgaccgaat ttgcagaaca atgccgttac
    59101 gaagaagaca ttgcacaact gcgccagtta ctgaaacagc tgaagcgtta tttgcaggat
    59161 aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc tctgtcaccg catcagtgag
    59221 tcagaggtta cccccgtcgt ctgcgacaac attggtgaaa gcacattgat tccactggcg
    59281 acctggtcga agtggtgttg tctgcgtaag caagagcggt tatggaaacg atttatcgcc
    59341 caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg
    59401 gccctcactt cgcgcgaagc ttaatcaagc tggtgttgtt gcataaacgg ctcaccgcct
    59461 aactgataca tctgccgtaa aatccacgcc tgacggctac gcacgtagcc cgatggcgcg
    59521 gagactttga aacgaagtgg attaggtaat acagctgcca gtaatgcagc ttccgaccgg
    59581 gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac gccaaacaca
    59641 ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt gctccagact
    59701 gtttctatcc ccagcgttaa tccggcttcc agcccttttc gaacccagct acgcccatcc
    59761 cataaaaaga gatttttggc tgtctgctga gaaatcgttg aagcaccgcg aatacggttt
    59821 tcattgcgct cgttgcgcgc cagggcttgc tcaatggaag cgacatcaaa gccccagtgc
    59881 tcaggaaatt tctgatcttc tgcggcaatc accgccagtc ccatccacgg cgagatttga
    59941 tccatactga cccagtcaga atgtgccacg taacgaaaat tgccatgcag ccaggcgctg
    60001 acttgccgct cgaccattac tgctgagaaa ggaacaggcg caacgctaaa caacgcgatg
    60061 cccccgcccc agaaaatggc gaggacaacc attaaccgca gaagaaaacg gcgaacgaaa
    60121 ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg gaaaccagct
    60181 tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgcc ggggtggtga
    60241 caatcttatt gtcttcatca accacgatat catcgacagg acacggcaca tgctccgcgc
    60301 ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa cgtagcggga
    60361 aatcgaaaat tttcggcagt atcgccggtg caatacacat aaaaccaagc ggttttccgg
    60421 cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat tcgctaccaa
    60481 gactggcaaa attgcttaaa ttcttcgccg cgccaaaacc ccccggcaca atcaacgcat
    60541 ccagttcagc ggcatcggcc tgggccagag gacgaatttc accgcgcgtt attcgtgccg
    60601 cttcaatcag cacattgcgc gtttccgtca tcgcttcacc agttaaatgg ttgataacat
    60661 caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta
    60721 acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat ccgctcagaa
    60781 ttacgccaat tttcttcatt gtgatcttcc tttcgcaatt gactgaaaca cattaatttt
    60841 tttaataaaa atgctacgca tcacacattt aactgattca tgtaacaaat catttaagtt
    60901 ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa
    60961 taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc accccggtct
    61021 agccggggtc attttttagt ggcttttgcc acccacgctt tcagtacttc tacgtcgtga
    61081 cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca ggcgggaagg
    61141 tcaggagact gaatttgctg acccagctgt tgcagatggc gtaaccccac tgaccccgcc
    61201 gcacctttaa ttttatgccc ttcctcaaca atgccttttt tatcctgcgc cgtcagattc
    61261 gactccagca cgctgacata gcccggcatc atcctctcaa acaccgctaa cccgtcggtg
    61321 atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc gagcaatgct
    61381 tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg ggtatcccag
    61441 aatttcttga tcatcgcggt tagcgccgga acagaaagcg gcttactcag cacatcatcc
    61501 attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc
    61561 ggcggtaaat cctcgcgcgg ataacgcttc gtcagtgcac gagagatatc caacccggtc
    61621 atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt aaacatctcc
    61681 agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaacttttc cagcacagag
    61741 cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag cgccggtaaa
    61801 ggcatatcgt cttcatcaaa cgcatcatca acctcttctg ctaccgacgg tgcgtggatc
    61861 gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa ccgtaatatc gccgcccata
    61921 tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc aggtttaccg
    61981 ccatgactgt ctttcacctg gtaatacatg gcaaagattt tatccagctc atcctgcgga
    62041 atgccgatac cagagtcttc cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg
    62101 cgcacggtaa cctggccttg ctgagtgaat ttgacggcgt tactgatgag gttccacaag
    62161 atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag cgtcggctcc
    62221 agattaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc cagatcggca
    62281 aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg ttccatctta
    62341 tccatgtcga taatatcgtt gaagatattc cccagcgtga cagccgaaac atggatggtt
    62401 ttgagatatt tttcctgctc ggcggtgagt tcagtatcca gcagaatgcg gctcaggccg
    62461 acgataccgt tcagcggtgt acgcaattcg tgactgatgg tggagataaa cgtcgttttg
    62521 tcgcggctgg cgcgttcaag cgcatcctga taccgcttac gctcggtaat gtcgcgacca
    62581 aagcccatca aaccgtgacg tttacccacg cggtcgtagt acggtacttt acggatttca
    62641 aagcaggctt tgcgtccgtc gggataatcc agccactgct cataggtcag tgacacatta
    62701 tgacggaaca ctttttcatc ggtttcaatg actttcgcag cggcttccgg tgagtaaaca
    62761 tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag ctccatcgcg
    62821 cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc gggtgaagcg
    62881 tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc
    62941 tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc cgctttttca
    63001 cgaacggcga tttcctgatt tagctgggca atattatctt ttaactgaac gttgaggctc
    63061 aaatcgcgct cgcgcatctc ctccagtttt tgcaccagtc gtgacagacg ttgtcgtgac
    63121 tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt aatcagcaaa
    63181 ccaaagaaga tagaacgaat aacatcaatg ctttcgacct ggccatgcag caccatggtt
    63241 accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa cattgagaag
    63301 cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag acgaatttgc
    63361 ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct gacagttacg
    63421 ttataaattg tgtgagaaat gcggaattcc cctcaatggc ctacgccgtt ggaggtagcc
    63481 acggacgtcc cagcgccgat ccctgtacgc catgttcatt cagataacga tccagcccga
    63541 ccattcccgt ccatcgattt tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc
    63601 tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt tctccggcag
    63661 tgagcgtgta atcctccagt tcaattccgt tcaaacgccc cgcttcccag gctttcgcca
    63721 taatgctgcc tttcactata tgcagcggat gcagctttat gccgtccacg ccggtttcaa
    63781 ccactcgctc cagcgtttgc aaacattcag cctgcccttc gccaggcagg ccaacaatta
    63841 agtgagaaca caccttcagc ccacgctcac gcgccagctg cgtcgtgcgc tgatagcagg
    63901 caaaatcatg accgcgattg atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca
    63961 gctccagcca cacttcgtag ccctggtcct tatattcgca aagcagatcc agcaccgcat
    64021 ccggcacgca gtccgggcgg gtaccaacac acaaaccgac aatattggcc tggctcaccg
    64081 cctgctgata catcgaacgc agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa
    64141 agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg
    64201 caatggaacg atgctgctgc gcttcatcgg caaacgaggc aacattacag aatgtgcagc
    64261 cgccacgccc gatggtaccg tcacggttag ggcagctaaa accgccatgc agcgtcagct
    64321 tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg actaatttct
    64381 gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc catttttagc
    64441 ccaattcggc gatgacctgg atcaatcgtc ccggcctgct tttatcaact gcataatcaa
    64501 tcaaaattac cgaaatttca tgcacaatca cataaatcac ttttgcttat cttgtgtcag
    64561 atttttttat ctccttatgg attttaggca aaaacagtag catgaaacgt tattaccaat
    64621 taaggcagta taaaatgctg gttttctcgt cagttcaagg caggataagg gttaacacgc
    64681 ctttatgaca gtcaggtatt ggctgtttct ctaacgacgc cccttttagc cttaaataga
    64741 aaatccaatt taatttcaga taattaataa agaattttgc gctaaagcac atttctgtac
    64801 caataagctt gccatttgac ctgtatcagc tttcccgata agttggaaat ccgctggaag
    64861 ctttctggat gagcagcctg ctcatcatat ttatgcagta attgagatcc cctcttcacc
    64921 gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc
    64981 aaaccccgtc gccacgctct tactgtgccc gtgcgcagcg gttcggaagt ggggttcccg
    65041 cagagcctgg gggaggttca cgatatgttg tacgataaat cccttgagag ggataactgt
    65101 ggtttcggcc tgatcgccca catagaaggc gaacctagcc acaaggtagt gcgtactgca
    65161 atacacgcac tggcccgcat gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggt
    65221 gacggttgcg gcttgctgtt acaaaaaccg gatcgttttt ttcgcatcgt tgcgcaggag
    65281 cgtggctggc gtttagcaaa aaactacgcc gttgggatgc tcttcctgaa taaagatcct
    65341 gaactcgccg ctgccgcacg ccgcatcgtt gaagaagagc tgcaacgcga aacgttgtcg
    65401 attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc
    65461 tctctgccac gcattgagca aatttttgtg aacgccccgg caggctggcg tccgcgcgat
    65521 atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa
    65581 gacttctacg tctgtagcct gtcgaatctg gtcaatattt ataaaggtct gtgtatgccg
    65641 gcggatctgc cgcgcttcta cctggacctc gcggacctgc gtctggaatc ggccatctgc
    65701 ctgtttcacc agcgcttttc cactaacacc gtaccgcgct ggccgctggc gcagccgttc
    65761 cgctacctcg cgcataacgg tgaaatcaac accatcactg gtaaccgcca atgggcgcgt
    65821 gcgcgtacct ataaattcca gacgccgctg atccctgacc tgcacgacgc cgcgccgttc
    65881 gtcaacgaaa ccggctctga ctccagttcg atggataaca tgctggaact cctgctggca
    65941 ggcgggatgg atatcatccg tgccatgcgt ctgttagtgc caccagcctg gcagaacaac
    66001 ccggatatgg acccggaact gcgtgccttc tttgacttta actccatgca tatggagccg
    66061 tgggatggtc cggcagggat cgtgatgtcc gatggccgtt tcgccgcctg taacctcgac
    66121 cgtaacggtc tgcgtccggc acgctacgtc atcaccaaag ataaactgat cacctgcgcc
    66181 tctgaagtcg gtatctggga ttatcagcct gacgaagtgg tcgaaaaagg ccgcgtaggg
    66241 ccaggcgaac tgatggtgat cgacacccgc agtgggcgca ttctacactc ggcagaaacc
    66301 gatgatgacc tgaaaagccg ccatccctat aaagagtgga tggagaaaaa cgtccgccgt
    66361 ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta gccgcgaact ggacgacgac
    66421 acgcttgcca gctaccagaa acaatttaac tacagcgcgg aagagctgga ttcggtgatc
    66481 cgcgtactgg gtgaaaacgg tcaggaagcg gtcggttcga tgggcgacga caccccattc
    66541 gccgtgctct ccagccagcc gcgcattatt tacgactact tccgccagca gtttgctcag
    66601 gtaaccaacc cgccaatcga cccgctgcgc gaagcgcatg ttatgtcgct cgccaccagt
    66661 atcggtcgtg aaatgaacgt cttctgtgaa gcagaaggcc aggcgcaccg tttaagcttt
    66721 aaatcgccga ttctgctcta ttccgatttc aaacagctca cgacgatgaa agaggatcac
    66781 taccgcgcag atacgctgga tatcaccttt gacgtcacta aaaccacgct cgaagcgacg
    66841 gtgaaagagc tgtgcgacaa agccgaaaaa atggtacgta gtggcaccgt gctgctggtg
    66901 ctctccgacc ggaatatcgc taaagatcgc ctgccggttc cggctccgat ggcggttggc
    66961 gcgatccaga cccgtcttgt cgagcaaagc ctgcgttgcg atgccaacat catcgtagaa
    67021 accgccagcg cccgtgatcc gcaccacttc gccgtgttgc tgggcttcgg tgcgacggct
    67081 atttatccgt accttgccta tgaaacgctg ggccgcctgg tagacaccca cgcgattgcc
    67141 aaagattatc gtaccgtgat gctcaactac cgtaacggca tcaacaaagg cttgtacaaa
    67201 atcatgtcca aaatgggcat ctccaccatc gcctcttacc gctgctcgaa actgtttgaa
    67261 gcggtcggtc tgcacgatga tgtcgtaggc ctatgcttcc agggcgcggt tagccgcatt
    67321 gggggggcga gctttgaaga cttccagcag gatctgctga atctgtcgaa acgtgcctgg
    67381 ctggcgcgta agcccatcag ccagggcggc cagctgaaat acgtgcacgg cggcgaatac
    67441 cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg cggtacagag cggcgagtat
    67501 agcgactatc aggaatacgc gaagctggtt aatgagcgtc cggcaaccac gctacgcgat
    67561 ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg ctgatgttga accggcaagc
    67621 gaactgttta aacgcttcga taccgccgcg atgtctatcg gcgcgttaag cccggaagcc
    67681 cacgaggcgc tggcggaagc gatgaacagc atcggcggta actcaaactc cggcgaaggc
    67741 ggtgaagacc cggcgcgcta tggcactaac aaagtgtcgc gcatcaagca ggtggcgtcc
    67801 ggtcgctttg gggttactcc ggcgtatctg gtcaatgccg acgtcattca gataaaagtc
    67861 gcccagggcg cgaagccggg tgaaggtggt cagttaccgg gtgataaagt cactccttac
    67921 attgccaaac tgcgctattc ggtgccgggc gtgacgctga tctccccgcc gccgcaccac
    67981 gatatctact ctattgagga tttagcgcag ctgattttcg acctcaagca ggtcaatcca
    68041 aaagcgatga tctccgtgaa gctggtttcc gaaccgggcg taggcaccat cgcgactggc
    68101 gtggcaaaag cttatgccga tttaatcacc atcgcaggct atgacggcgg caccggcgca
    68161 agtccgcttt catcggtgaa atacgcaggc tgtccgtggg agctggggct tgttgaaacc
    68221 caacaggcgc tggttgccaa cggcttgcgt cacaagatcc gtttgcaggt cgatggcggc
    68281 ctgaaaacgg gtgtcgatat catcaaagca gcgattcttg gcgcggaaag ttttggcttc
    68341 ggcactggcc cgatggtagc gcttggctgt aaatatctgc gaatttgcca cctgaacaac
    68401 tgcgccacgg gtgtagcaac tcaggatgac aaactgcgta agaatcacta tcacggcctg
    68461 ccgttcaaag tgactaatta ctttgagttt atcgcccgtg aaacccgcga gctgatggca
    68521 cagctggggg taacgcgtct ggtggatctg attggtcgca ccgacctgct gaaagagctg
    68581 gacggtttca ccgccaaaca gcagaaactg gcactgtcga agctgctgga gactgccgag
    68641 ccgcatcctg gtaaagcgct ctactgcacc gaaaacaacc cgccgtttga taacggcctg
    68701 ctgaacgcgc agttgctgca acaggcaaaa ccgtttgtcg atgaacgcca gagcaaaacc
    68761 ttctggttcg atatccgcaa caccgaccgt tctgtcggcg cgtcgctttc aggctatatc
    68821 gcccagacgc acggcgatca ggggctggct gccgatccta tcaaagcgta cttcaacggc
    68881 accgcaggcc agagcttcgg cgtgtggaac gcgggcggcg tggaactgta cctgaccggt
    68941 gatgccaacg actatgttgg taaaggcatg gcgggtggct taatcgccat tcgtcctccg
    69001 gttggttccg ccttccgcag ccatgaagca agtattatcg gcaacacctg cctgtatggc
    69061 gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcgtaac
    69121 tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg gctgtgaata tatgacgggt
    69181 ggtatcgtct gtattctggg taaaaccggc gttaacttcg gcgcgggcat gactggcggc
    69241 ttcgcttacg ttctcgatga aagcggcgat ttccgcaaac gcgttaaccc ggaactggtc
    69301 gaggtcttaa gcgttgacga tttggcgatc catgaagagc atctgcgtgg tcttatcacc
    69361 gagcatgtgc agcataccgg ttcccagcgc ggtgaagaga ttctggcgaa ctggtcaacc
    69421 ttcgccacga aattcgcgct ggttaaaccg aagtccagtg atgtaaaagc actgctgggt
    69481 caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg
    69541 agtcagaatg tttatcaatt tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg
    69601 aagatccgca aaattgagtt tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa
    69661 gcgcaggctg accgctgcct gtcgtgcggc aacccatact gcgagtggaa atgcccggta
    69721 cacaactaca tcccgaactg gctgaagctc gccaacgagg ggcgtatttt tgaagcggcg
    69781 gaactgtcgc atcagaccaa taccctgccg gaagtgtgcg gacgtgtctg cccgcaagac
    69841 cgtctgtgcg aaggttcctg caccctgaac gatgagttcg gcgcagtgac catcggcaac
    69901 attgagcgct atatcaacga taaagcgttc gagatgggct ggcgtccgga tatgtccggc
    69961 gtgaaacaga ccggtaaaaa agtggcgatt atcggcgcag gtccggcggg tctggcgtgc
    70021 gcggatgtcc ttacgcgcaa cggcgtaaaa gcggttgtct tcgaccgtca cccggaaatt
    70081 ggcggcttgc taaccttcgg tattccggcc ttcaagctgg aaaaagaggt aatggcgcgc
    70141 cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac tcaataccga agtgggccgc
    70201 gacgtacagc tggacgatct gctgagtgat tacgatgccg tgttccttgg cgtcgggact
    70261 tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg atggcgtgta cgcagcgctg
    70321 ccgttcctta tcgccaacac caaacagtta atgggctttg gcgaaactcg cgaagaaccg
    70381 ttcgtcagca tggaaggcaa acgcgtggtg gtccttggcg gtggcgacac tgcgatggac
    70441 tgcgtgcgca cgtctgtacg ccagggggca aagcacgtta cctgtgccta tcgtcgtgat
    70501 gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag
    70561 ttcaaattca acgtccagcc actggggatt gaagtgaacg gcaacggcaa agtcagcggc
    70621 gtaaaaatgg tgcgcacaga aatgggcgaa ccggatgcca aaggccgtcg ccgcgcagag
    70681 atcgtggcag ggtcagaaca tatcgttccg gcagatgcgg tgatcatggc gtttggtttc
    70741 cgtccacaca gcatggaatg gctggcaaaa cacagcgtcg agctggattc ccaaggccgc
    70801 atcatcgctc cggaaggcaa cgacaacgct ttccagacca gcaacccgaa aatctttgct
    70861 ggcggcgata tcgtccgagg ttccgatctg gtggtgaccg ctattgccga aggtcgtaag
    70921 gcggcagacg gcattatgaa ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc
    70981 agaaaagagt cggcgctgaa attctccttt ctttctggaa agcgcctcgc ataatctgaa
    71041 gaaaaccaat gtaatacgct catctggatt gttaccggta gatgcccggc gtatagctga
    71101 ttccattatt caacgcagcg ccatataaag ggacagataa tggaatcgct ctcagagaga
    71161 acctcaacag gctaccagca aatccacgac ggtattattc acctggtcga tagcgcccgg
    71221 acggaaacgg tacgtagcgt taacgcgctg atgaccgcga catactggga aattggccga
    71281 cgaattgtcg aatttgaaca aggtggcgag gccagggctg cgtatggtgc gcagctaatc
    71341 aagcgaccat caaaggattt aagtctaagg tataagcgtg ggttctctgc aaaaaactta
    71401 cgccaaatga ggctttttta cctctttttt caacatgttg aaattcgcca gacagtgtct
    71461 ggcgaattaa caccattggg aattccgcag acaccatctg cagaattccc atcggcaaag
    71521 atttggcaga cgctgtctgc caaatctttc cccctgccct ggtccactta cgtccgtttg
    71581 ctttctgtta aaaacgctga cgctcgtagc ttttatgaaa aagagacgct ccgctgtggc
    71641 tggtctgttc gccagctaga gcggcaaatt gcgacccaat tttatgagcg aacactactg
    71701 tcacatgaca aatcagccat gctgcaacaa cacgctccta ccgagacgca tattcttccg
    71761 cagcaggcga tacgcgatcc ctttgtgctc gaatttctgg aattgaaaga tgaatattca
    71821 gaatctgatt tcgaggaggc gctgatcaac cacctgatgg atttcatgct ggaacttggg
    71881 gatgattttg cctttgttgg tcggcagcga aggttacgca ttgatgacaa ctggtttcgt
    71941 gtcgatctgc tgtttttcca ccgccgttta cgctgcctgc taatcgtcga tctaaaagtg
    72001 ggcaaattca gctatagcga tgccggacag atgaatatgt atctcaacta cgccaaagag
    72061 cactggacgc taccggatga aaatccgccc atcggtctga ttctctgtgc agagaaagga
    72121 gccggcgaag cgcattatgc actggctggt ttgcctaaca ccgttctggc gagcgaatat
    72181 aagatgcaac tacctgatga aaaacgactc gcggatgaac tcgttcgaac acaggcggtg
    72241 ctagaggaag gctatagacg ccgttaattt cagcggtttc gaattctgca gacagtgtct
    72301 gcagaatctt taaaacgaac taaattttaa gccattaaat cagccttaat cttcactacc
    72361 acctttttaa tctcaccagg ttcgccaaca acgcatcccg gtttatgcgg ttctcctggc
    72421 ataaacacgg cgaacattcc cggtttcagg atgatggctt gctcgttttc aatggcgctg
    72481 caaagctgat aatcatcttc atggtggaac tcttcacact gacgcgcagt gcctgccatg
    72541 ccaaacagaa tccgttcctc accgtttaat aacagctgga tatcaatgta ttgttcgtgc
    72601 aattcggctt ttttctcaac gggcgattga gtgttaaacg tcatgacatt cataaaaata
    72661 ttgtcgccct gtaattcgta acggccagac gctttttctt gcggtctggc agctaatgcc
    72721 agcgttaacg cgccctgtaa cgcaggatgt aatccggcag acggtaatga ctgtacttca
    72781 cccatcatca taatatttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcgt
    72841 ggcggtagtg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagatacg
    72901 tttccaccag cgccagatac ccttctgcca gaccaacgct gccaccgacc accacgcact
    72961 ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt gcggagcggt
    73021 gaatcagttg ctgcgcctgc tcatcgccct gcccggcgcg cgtgaaaata gttttcgcat
    73081 tcgcaccagc caactccccc tgcgctgccg ctgcaatgcc gcgaccagaa gcaatcgctt
    73141 ccacgcaacc tgtgcgtcca cagccgcaga ctggaccatg cggatcggca agcgtatgcc
    73201 cgatatgccc cgccagaccg ccagggccgg tacgcagttt accgccgctc actacaccgc
    73261 cgccaacgcc ggtggaaaca gtgataaaca ccatatcggt tatatcgcca tccagcgcct
    73321 gatactccgc ccatgctgcg gcctgcgcat cattaatggc aatggtcggc aaattggtaa
    73381 gttgctccag cgttttgact aacggaaagt gtaacaatcc accgagatta tgcggattca
    73441 gcgccaacaa gctgccgtcg cgaataattc cggttgaagc gatggcaacc cgctgcgcat
    73501 gtgcttgcaa cggagagact aatgcggata aggcatcacg cagcgcctgc ggagtctggc
    73561 tggcaggcgt aggaagttct cggcgatcgc ggatctgccc gtcagcgcca atcagcgcgg
    73621 cggcaagttt agtaccgccg atatcaatcg ccagtgtggt catagcaccg cctttttcat
    73681 cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac
    73741 cgcccacgcg ccgtggcgca tcgcatccgc cgcctgagca ggcgtgttgt aacgcccttc
    73801 ggcaatcacc cggcatccgg cgtcactcaa cgttttcacc agcgccagat ccggctcttc
    73861 tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt
    73921 ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg
    73981 gtgaatacgt gccagcagcg tttcaacagg taccggacgc ggtcggtcgg tgccgtcaat
    74041 ggcgataatg tccgcgcccg cctgcgccag cgcatcaaca tcttcaatat aggccgtgat
    74101 gcgtaccgga gaatcctcca tatcgcgctt cacaatcccg ataatcggca cgctcaccac
    74161 cgcacgcgtg gcttgcagat ttgccacacc ttcaatacga atggcgaccg cgcccgcctg
    74221 ctctgccgct aatgccatgg cggcgacgat ttcgggttta tcgagcgggc tgtccggaac
    74281 cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag
    74341 taacgacata catcttccct tagcgaaagg cccggtacag agaccgggca acaggattaa
    74401 cttttggttt tgactaaatc gtttttggcg ctgccaaacg gcacggcacc gctaaatggt
    74461 ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacacga
    74521 gaaggcatat ccaaaccaat cagcaggatc accacaaacg ttaaactaaa cgagagcgag
    74581 ccaagtgccg ttcccagatc cagacgttga gcgatcaacg cgccgaggat tggagctagt
    74641 gcaccgccca gtgcgccaac gttgtaggta aagcccagcc ccgctgcacg ctggtcggta
    74701 tcgaaataac cgccgatcag tttcggtaaa atccctgaga tgccctgacc aagcatttgc
    74761 tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga
    74821 ataatcagca actgcgaggc cagcagacta caaacgtacg ctttgcgggt tcccagccag
    74881 tcaccgagga agccaccgac gcagcacccc accgccgcgc caaagccact aaagaacagc
    74941 acattggcga cagtatgcgg gtcataagcc agatcggttt tcagatacgt tggcagcagc
    75001 gcctgaatcg gccatgagta gaggaaagcg aacaacacga ccaccatcag cattacgccc
    75061 gttggccagc gtttgcccgt actctgcacc ataaagctga taaagattgc ggcgcataac
    75121 agcccaagaa cagcgacgat cgcggcattt tgcaggttac cggcgaagca gaaccacagc
    75181 gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga
    75241 atgtctacca tcgtgcgtac tggtgcttta cccccgtgtt tctctttcca gtcttccgct
    75301 tccgggatgt ttttacgcag ccagagagca aagatgattg gcaaaatgcc gataaagaac
    75361 agcgcacgcc agccccagac cggaaccacc agactataga cctgagcggc aacgacggcc
    75421 cccactgaga agcctgaaat cagaaaacca ctggctttgt tacgcagatg ctttggccag
    75481 ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat gccaatgacc
    75541 agacgagcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc
    75601 gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg
    75661 ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc
    75721 tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga gtaccagggc gattaaaacg
    75781 aaatcaaaac cgtcaagcag atatcccaac caggctgcgg aaaatgcccg ccattgtgcg
    75841 cggttaagat ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg
    75901 cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg cgtttatttc
    75961 ccctcacccg gtaggggcga gcgaggggaa acaactcacc cgcgctcttg catcaactgc
    76021 tgggccagcg ccttcagttc tggcagatat ttttcatcta ccggaccaaa cggtttgcgg
    76081 cacagcggca cagaaacgac atccatataa tgcaggacag ttttcaggcc gcggaatacg
    76141 cccgttttga tcagtaaatc aatgactttg ttgcactcag tttgcagttt ctgcgcggtc
    76201 tggatatcgc cttctttcag cgccttcacg atcccctgat aacgccagcc catgatgttg
    76261 taagtgctgc cgataccgcc gtctgcgccc gccagaagac cagaggcgaa gatttcgtcg
    76321 taaccgttat agagcaccag atcaggatgt tcacgacgga tctgctccat ctgatagaga
    76381 tcgccagagg tctgtttcag cgcacctaca ccaggcagcg taaccagggt gttgatctga
    76441 tccagggtca gttttacccc gctcagggct ggaatgttgt acaccaccat cggcaaccca
    76501 tccgccgaat caataattgc ccgatagtga tcgcagtgtt cttcaaagct gaaaggatag
    76561 tagaacggcg tgacggcgga gacggcatcg aagccataac gtttagccga tgccgcaagt
    76621 tgttggcttt cggcggtgct gacgcaaccg acgtgggcga tgagtttaat cttacctttc
    76681 gcctcttcag cgacgatttc cagtacctgt tcacgctctg aaaggctctg tacaaaggct
    76741 tcgcctgtcg aaccacccac gtacaaaccg tcgatgccct gctgaatatt gaactgaacc
    76801 aggcggcgca gactcgcttt atccagtgct tgttgttggt caaaaggagt caggagtgca
    76861 gccattacgc cacgtaaatt cgttgccata aatacctctg aagtgatgct tgtctgataa
    76921 acgatatacc tttatacctg ttataccaga tcaattaagc aacaccccat acagaaagct
    76981 tataatgcga tctgcttcac taaagtggca ttatttcttt ttgttggtgg tctgaccgaa
    77041 ggcgtgccag gtagcagaga cgctgttgag atgcgattgc aacgcgcgat cggcttcgtc
    77101 aggatcatga cggcggatcg cgtcaacgat cgcaatatgc tgttgatagc taacgttgtt
    77161 atgctcatgc agagcctgat cggtcacggt tggacgtgcg gcgattagcc agtcgagcag
    77221 ggcaacgtgg atcgccatga agattgggtt accagggatc tccgccagaa cgcggtggaa
    77281 atcaacgtct gagcgaatga atgccgcgtt gttatccagc gactgactgt tgatttccag
    77341 tgcttttgcc agcaaatcga tttgctcatc ggtggcgtgt tcagccgcat agcgcaccag
    77401 gctggattca aagaacagac gtaattgttc gaaatgggca atcccaccag gatgagaaag
    77461 gaaatctttc gccatgccgg aaagctcgcc gatgatggtg tccgcagaag gacgtgagac
    77521 gcgggcacgt tcgccgttgt ttatttgcac cagacctttg cgttttaacg ctgccagcgc
    77581 ttcgcgcacc gaaggacgcc cgacgttaaa gaacgccatc agttcgcgtt cagacggtaa
    77641 ttgttcacct tcgccaaatt cacgacggcg gatcatctgt tccagctctt cttccaccat
    77701 ttcggagagt tttttacgcg ccagcgggcg gctgcgcaag ttgcgcccga tagtggtagt
    77761 agaatcttca gcttgcggat caaatgcgtt cataggactc attcaggaaa atgcgttagc
    77821 ggataataac gacgctatcg tagcacagta tcaaagtcag gcgaaaagat gacagatagc
    77881 aaaacaggcc acatgggcct gttttcattt tacttcacaa cgcgtaatgc cggtcgacca
    77941 ccgcgtggtg gctgcggagg ttcatcgtca ggatgagttt catcatcgtg atctggcttg
    78001 tcgccatcga taaccgacat aacggtttcg ttgtctgccg atgcctcttc atcattcatg
    78061 atgctggcat cttcatcgta ggcagcttca ggctcaaaca tcgtgcctgc gccattttca
    78121 cgggcgtaga tagccagcac ggcagccagc ggcacagaaa cctgacgcgg aatgccacca
    78181 aagcgcgcgt taaagcgcac ctcatcattc gccagttcca gattgccgac agcacgcggc
    78241 gcaatgttga gtacgatttg cccgtcacgc gcatattcca taggaacctg cacgcctggg
    78301 agcgtcacat ccaccaccag gtgcggcgtg agctggttat ccagcaacca ctcatagaat
    78361 gcacgcagca gatagggacg acgtggtgtt agctgtgaca aatccataca gattaactcc
    78421 ggcccagacg catttcacgt tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa
    78481 atacgcgggt catatagcct ttcagctctt tcgcacccgg gccgctgaac tcgatgccca
    78541 gttgcggcag acgccacagc agaggagcaa ggtagcaatc gaccaggctg aactcatcgc
    78601 tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt
    78661 gcttacgtgc ggcatcggct tcagaagctg aaccgttgat gatggtgttc atcagcgtgt
    78721 accagtcttt ttcgatgcga tgcatgtaca gacggctttc accgcgagct accgggtaaa
    78781 caggcatcag tggcggatgc gggaaacgct catccagata ttccataatg atgcgagatt
    78841 cccacagggt cagctcacga tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa
    78901 tcagatcctg aggcggattg tccttttcca cgtgttcgat ctcgaaactt acacctttct
    78961 cagccagcac aatgcggacc tgatggctat agatgtcagt aggaccggaa aacagcgtca
    79021 ttaccgaacg tttgttggca gcgacagcca tgaaaacctc caggtatagt cagaattttt
    79081 actgctacca gccaccaggt ggccagtcag aagttgtgtt acccagtaag gaacgactct
    79141 ctttgttcga aaatcaaaca aaaaatgagc aatacccgac atttgggcag aaaattggat
    79201 gatagtttac cagattttgc gacctttgtg gtgagtcgat gccggaaatg gggaaaaaga
    79261 gatgcgcttt agtctgaaat agttgactta gtcccttatt ggcgatgtgg tttttgtttt
    79321 acctgtctgt caggtggcag caaaaagcaa ctttccattt tttacgctga tgcagatttt
    79381 agccataaaa aaacccgccg aagcgggttt tttcgcaaat tgttttctgc cggagcagaa
    79441 gccaattaac gtttggagaa ctgcggacga cgacgtgctt tacgcagacc gactttctta
    79501 cgttcaacct gacgagcgtc acgagtaacg aagccagctt tacgcagttc agaacgcagg
    79561 gactcgtcgt attccatcag agcgcgggtg ataccgtgac ggatcgcacc agcctgacca
    79621 gagataccac cacctttaac ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt
    79681 tccagcggct gacgaactac catgcgagca gtttcacgac cgaagtactg ttccagagaa
    79741 cgttggttga ttacgatttt accgttgccc ggtttgatga aaacgcgagc tgcggaactt
    79801 ttgcggcgac cagtgccgta gtattgattt tcagccattg cctataatcc cgattagatg
    79861 tcaagaactt gcggttgctg tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt
    79921 ttacggaaca tagcacgacc cagcgggcct tttggcaaca tgcctttaac cgcgatttca
    79981 atcacacgct caggacggcg agcaatcatc tcttcaaagg tcgcttgttt gataccaccg
    80041 atgtggccgg tgtggtgata gtacactttg tcagtacgct tgttgccggt tacagcaact
    80101 ttgtcagcgt tcagaacgat gatgtaatca ccggtatcta cgtgcggagt gtattccgct
    80161 ttgtgcttac cgcgcaggcg acgagccagt tcagtagcca gacggcccag agttttaccg
    80221 gtcgcgtcaa caacatacca gtcgcgtttt acggtttctg gtttagctgt aaaagttttc
    80281 attaaaagct tacccaataa atagttacac gttggtgaac acccaaacgt cttcaattgt
    80341 tgaggttcac acgacaaagt ccggcaaacc taccccttcg aatagcctat gccagcacac
    80401 aaaaagtttc gggaaaaaaa actttcttgt aacgtggggt cgcaggatta tagagaagtc
    80461 ggggtcaaag atcgacccct ttttgtgatt tgtggcaggt tttaccccgc caaatgctcg
    80521 cgcttcaaat actcttcgct ttgcatctct tgcagacgtg acaggcaacg ctggaactca
    80581 aacttcagcc gctcgccctg ataaatttca tacagcggca cttctgcact caccactaat
    80641 ttgacatggc gctcgtaaaa ctcatccacc agcgcaataa agcgtcgcgc ttcgctctcc
    80701 atcaaccgcg tcataactgg tacatcaaac aacatgaccg tgtgaaagag gcgtgagagc
    80761 gcaatatagt catgctgact gcgggcgtcg acgcacagcg tagtaaaaga aaccgccagc
    80821 gtctggttct cgacgcccat tgtcgccaat ggccgatggt tgatttctaa cgtcggtgaa
    80881 ttttctcgtt tcgcccccgc cagcgccaac catagtttat ccatttgcgc ccgggtttca
    80941 tcgttgaggg gcgaaagcca cagatgcgcc tgagtgagtg tacgcagtcg ataatcaaca
    81001 ccagcgtcca cgttcattac atcacaatgc tgtttaatag catcgattgc aggcaaaaaa
    81061 cgcgcacgtt gcaggccatt tcgataaagt tcgtccggcg gaatatttga cgtcgctacc
    81121 agcgttattc cgcgagcgaa cagggctttc atcaggccgc caagtagcat ggcatcggta
    81181 atatcagaaa caaaaaattc gtcaaaacag agcacgtcag tttctgcttt aaagcgatcg
    81241 gcaataattt ccagcggatc ggtctgcccc tgcaaagcag ttagctcttc atgtacccgc
    81301 agcataaaac ggtgaaagtg cagacgctgt ttccgctctc ccggcaggct ttgatagaaa
    81361 aggtccatca gccaggtttt cccgcgtcct acaccgcccc acatatataa gccacgcact
    81421 ggcgtatgct ttgtgtcttc gcgtttaccc cacagcttac cgacccgcgc cattagccca
    81481 ctcgtcctgg gggctggagg cgtgctattg atgagttcct gataaataat ttccaggcgg
    81541 ctgacggcct ctttttgaac gtcgtcgggt tgatggctgc cttcattgag cgccttcagg
    81601 tattgcgatg ttggggtaat gctttgcatg atcttattgt tattccttga ataatcggtg
    81661 cgccgttgtt cacggttgac gaaaaaaagg ccgttctaca ctacgcgata tgcagtcggg
    81721 attccacttc tgtggaatta acggttatag tggcataatc agccgcaggc atggagcctg
    81781 aagccaacac cctacggaaa caaaagacaa cgggagatgt tcatgacctg ggaatatgcg
    81841 ctaattgggt tagtcgtcgg catcattatt ggtgctgtgg ccatgcgttt tggtaatcgt
    81901 aaactacgcc agcaacaggc gttgcagtac gaactggaaa agaataaagc tgaactggac
    81961 gagtatcgcg aagagctggt tagccacttt gcccgcagcg cggaattact ggataccatg
    82021 gcgcacgact atcgccagct gtatcagcac atggcgaaaa gctccagtag cctgctgccg
    82081 gaactgtctg cggaagcaaa cccgttccgt aatcgtctgg cagagtctga agccagcaac
    82141 gatcaggcac cggtgcagat gcctcgcgac tattctgaag gcgcatccgg cctgctgcgt
    82201 actggcgcga agcgcgacta atttattttt cgggcgcagc cattgcgccc tcctcttctc
    82261 tccctccccg actatcattt aatctggtgt ctcattgtta gccgtctgaa aattcaataa
    82321 catcaaactg ttttgaatct cttttcttat cattcaggta cgagagcagg aataatgaaa
    82381 aaacaaaccc agctgttgag tgcattagcg ttaagtgtcg ggttaactct ctcggcgtca
    82441 tttcaggccg tcgcgtcgat tccaggccag gttgccgatc aggcccccct ccccagtctg
    82501 gctccaatgc tggaaaaagt gcttccggca gtggtgagcg tacgggtgga aggaacggcc
    82561 agtcagggac agaaaatccc ggaagaattc aaaaagtttt ttggtgatga tttaccggat
    82621 caacctgcac aacccttcga aggtttaggc tccggtgtca tcatcaacgc caataaaggc
    82681 tatgtgctga ccaacaacca tgtgattaat caggcacaga aaatcagtat tcagctcaat
    82741 gatgggcgcg agtttgatgc aaaactgatt ggtagcgatg accagagcga tatcgccctg
    82801 ttgcaaattc aaaacccgag caaattaacg caaatcgcta ttgccgactc cgataaattg
    82861 cgcgtcggtg attttgccgt ggcggtcggt aacccatttg gccttgggca aaccgccacc
    82921 tctggcatta tatccgcatt aggccgcagc gggttgaatc ttgaaggtct ggaaaacttt
    82981 atccagacag atgcttccat taaccgcggt aactccggcg gtgcactgtt aaaccttaac
    83041 ggtgagttaa ttggcatcaa cactgcaatc cttgcgcctg gcggcggtag cgtcgggatt
    83101 ggatttgcca ttcccagtaa tatggcgcga acactggcgc agcagcttat cgactttgga
    83161 gaaatcaaac gcggcctgtt aggcatcaaa ggcaccgaga tgagtgccga tatcgccaag
    83221 gccttcaacc ttgacgtgca gcgtggcgcg tttgtcagcg aagtgttgcc aggttctggc
    83281 tcggcgaaag cgggcgtcaa agcgggcgat attattacca gcctcaacgg caaaccgctg
    83341 aatagctttg ctgagttgcg ctctcgtatc gcgaccaccg agccgggcac aaaagtgaag
    83401 cttggcctgc tgcgtaacgg caaaccactg gaagtagaag tgacgctcga taccagcacc
    83461 tcttcgtcgg ccagcgctga aatgatcacg ccagcgctgg aaggtgcaac gttgagcgat
    83521 ggtcaactaa aagatggtgg caaaggtatt aagatcgatg aggttgtcaa aggaagccca
    83581 gctgctcagg ctggcttgca aaaagacgat gtgatcattg gcgtcaaccg cgatcgggtg
    83641 aactcgattg ctgaaatgcg taaagtgctg gcggcaaaac cggccatcat tgccctgcaa
    83701 attgtacgcg gcaatgaaag catctaccta ctaatgcgtt aatgtcgtaa accgggcatc
    83761 aggctgacgt gtgatgtccg gttaactcgt ggtatgctgc tgccgttccc ttttttaacg
    83821 atgcctccat catgtttgtg aagctcttac gttccgttgc gattggatta attgtcggcg
    83881 ctattctgct ggttgccatg ccttcgctgc gcagccttaa cccgctttcc actccgcaat
    83941 ttgacagtac cgatgagacg cctgccagct ataatctggc ggttcgccgc gccgcgccag
    84001 cggtggttaa cgtttataac cgcggtttga acaccaactc tcacaaccag cttgagatcc
    84061 gcaccctggg atctggtgta atcatggatc aacgcgggta tatcatcacc aataaacacg
    84121 tgatcaacga cgccgatcag atcatcgtcg ccttacagga tggacgtgtt tttgaggcgt
    84181 tactggttgg ctctgactct ctaaccgatc tggcggtgct gaaaattaac gccacaggcg
    84241 gtttaccaac gattccgatc aatccacgcc gcgtaccgca cattggcgat gtggttctgg
    84301 cgatcggtaa cccgtacaac ctcgggcaga ccattaccca agggattatc agtgccacgg
    84361 gtagaatcgg tctgaacccg accgggcggc aaaacttcct gcaaaccgat gcttccatta
    84421 accacggtaa ctctggcggc gcgctggtga actcgctggg cgaactgatg ggcattaaca
    84481 cgctgtcgtt tgataagagt aacgatggcg aaacgccgga aggtatcggc tttgcgattc
    84541 ctttccagtt agcgaccaaa attatggata aactgatccg tgatggccgt gtgatccgtg
    84601 gctacattgg cattggtgga cgtgagatcg ccccattaca tgcgcagagt ggcggcatgg
    84661 atcaactgca agggatcgta gttaacgaag tgtcgcctga tggcccagcg gcaaatgcag
    84721 gcatccaggt caacgatctg attatctctg tgaataacaa accggccatc tctgctctgg
    84781 agacgatgga tcaggtggca gaaattcgcc ctggttcggt gatcccggtt gtcgtgatgc
    84841 gtgacgataa acagttaaca ttgcaggtca ccattcagga atatccggcg accaactaag
    84901 gtcagcgtaa aaaaacaaaa accggagtct gtgctccggt tttttattat ccgctaatca
    84961 attacttatt aacgaactct tcgcccaggg cgatatcttt cttcagcgta tccagcatac
    85021 cttccagcgc gttctgttca aatgcgctca gagtaccgat agatttacgc tcttccacgc
    85081 cgtttttacc cagcagcagc ggttgagaga agaaacgagc gtactgaccg tcgccttcaa
    85141 cgtaggcaca ttcgacaacg ccttgttcgc cctgcagtgc gcgaaccaga gacagaccaa
    85201 aacgtgcagc tgcctggccc atagacaggg ttgcagaccc gccaccggct ttcgcttcaa
    85261 ccacctcagt acccgcgttc tggatacgtt tggtcagatc agccacttcc tgctcggtaa
    85321 aactaacgcc aagaacctgt gacagcagcg gcagaatggt aacaccagag tgaccaccaa
    85381 taaccggcac ttcaacttcg cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg
    85441 aacgaatgat atccagcgtg gtaacgccga acagtttgtt tttgtcataa acaccggctt
    85501 ttttcagcac ttcagcagca atcgcaactg tagtgttaac cgggttagtg ataataccaa
    85561 tgcacgcttt cgggcaggtt ttcgcaactt gctgtaccaa gtttttcacg atgccggcgt
    85621 taacgttaaa caggtcggaa cgatccatac ccggtttacg cgctacgcct gcagagataa
    85681 gaacgacatc tgcaccttcc agcgccggag tcgcatcttc acctgaaaaa cctttgattt
    85741 tcacagcagt agggatatgg ctcagatcga cagccacacc gggagtcact ggagcgatat
    85801 catacagaga gagttctgaa cctgaaggca gttgggtttt taacagtagt gcaagcgcct
    85861 ggccaatacc gccagcagcg ccgaggactg cgactttcat cctaaactcc ttattatatt
    85921 gataaactaa gatatgttgc tccgctgccg cgaccttaat ccacaaaatt gccgtttaca
    85981 atgaccacat ctcaagaatg tgtagtcacg caagtttagc gtttatgcat ttaattgccg
    86041 taatcaggaa cctaacttac gtaattaaca gtcacttatg cattagcgca acattccaac
    86101 aggtggtgac aatataccct accgctcagc caaaacaata tcaatttgat aacaattaat
    86161 ttacttttaa gcagaatttg caggccgtga cgcaggcatg tttctcaata acgaaatttg
    86221 ataaaatccc gctctttcat aacattattt cagccttctt cagggctgac tgtttgcata
    86281 aaaattcatc tgtatgcaca ataatgttgt atcaaccacc atatcgggtg acttatgcga
    86341 agctcggcta agcaagaaga actagttaaa gcatttaaag cattacttaa agaagagaaa
    86401 tttagctccc agggcgaaat cgtcgccgcg ttgcaggagc aaggctttga caatattaat
    86461 cagtctaaag tctcgcggat gttgaccaag tttggtgctg tacgtacacg caatgccaaa
    86521 atggaaatgg tttactgcct gccagctgaa ctgggtgtac caaccacctc cagtccattg
    86581 aagaatctgg tactggatat cgactacaac gatgcagttg tcgtgattca taccagccct
    86641 ggtgcggcgc agttaattgc tcgcctgctg gactcactgg gcaaagcaga aggtattctg
    86701 ggcaccatcg ctggcgatga caccatcttt actacccctg ctaacggttt caccgtcaaa
    86761 gagctgtacg aagcgatttt agagctgttc gaccaggagc tttaatctct gccccgtcgc
    86821 ttctggcggc ggggaaaatg ttgcttttgc ctatcaaccc cctgctttcc cctgcgattc
    86881 atttaacgaa tagtgcgttt tactgcgaca tatcattca
//
LOCUS       DAZKHK010000003        85649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKHK010000003 DAZKHK010000000
VERSION     DAZKHK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.003"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(195..1379)
                     /gene="araJ"
                     /locus_tag="ACHOS5_000164"
     CDS             complement(195..1379)
                     /gene="araJ"
                     /locus_tag="ACHOS5_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP9775060.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAIFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(1505..4648)
                     /gene="sbcC"
                     /locus_tag="ACHOS5_000165"
     CDS             complement(1505..4648)
                     /gene="sbcC"
                     /locus_tag="ACHOS5_000165"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP9775061.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAMK"
     gene            complement(4645..5847)
                     /gene="sbcD"
                     /locus_tag="ACHOS5_000166"
     CDS             complement(4645..5847)
                     /gene="sbcD"
                     /locus_tag="ACHOS5_000166"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP9775062.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDISQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            6037..6726
                     /gene="phoB"
                     /locus_tag="ACHOS5_000167"
     CDS             6037..6726
                     /gene="phoB"
                     /locus_tag="ACHOS5_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP9775063.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6784..8079
                     /gene="phoR"
                     /locus_tag="ACHOS5_000168"
     CDS             6784..8079
                     /gene="phoR"
                     /locus_tag="ACHOS5_000168"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP9775064.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            8486..9805
                     /gene="brnQ"
                     /locus_tag="ACHOS5_000169"
     CDS             8486..9805
                     /gene="brnQ"
                     /locus_tag="ACHOS5_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP9775065.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            9881..11254
                     /gene="proY"
                     /locus_tag="ACHOS5_000170"
     CDS             9881..11254
                     /gene="proY"
                     /locus_tag="ACHOS5_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP9775066.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            11410..13227
                     /gene="malZ"
                     /locus_tag="ACHOS5_000171"
     CDS             11410..13227
                     /gene="malZ"
                     /locus_tag="ACHOS5_000171"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP9775067.1"
                     /translation="MMLKAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIELSCGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRMLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(13232..13813)
                     /gene="acpH"
                     /locus_tag="ACHOS5_000172"
     CDS             complement(13232..13813)
                     /gene="acpH"
                     /locus_tag="ACHOS5_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP9775068.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            13906..14976
                     /gene="queA"
                     /locus_tag="ACHOS5_000173"
     CDS             13906..14976
                     /gene="queA"
                     /locus_tag="ACHOS5_000173"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9775069.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            15031..16158
                     /gene="tgt"
                     /locus_tag="ACHOS5_000174"
     CDS             15031..16158
                     /gene="tgt"
                     /locus_tag="ACHOS5_000174"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9775070.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            16181..16513
                     /gene="yajC"
                     /locus_tag="ACHOS5_000175"
     CDS             16181..16513
                     /gene="yajC"
                     /locus_tag="ACHOS5_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9775071.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            16541..18388
                     /gene="secD"
                     /locus_tag="ACHOS5_000176"
     CDS             16541..18388
                     /gene="secD"
                     /locus_tag="ACHOS5_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP9775072.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            18399..19370
                     /gene="secF"
                     /locus_tag="ACHOS5_000177"
     CDS             18399..19370
                     /gene="secF"
                     /locus_tag="ACHOS5_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP9775073.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            19500..19847
                     /gene="yajD"
                     /locus_tag="ACHOS5_000178"
     CDS             19500..19847
                     /gene="yajD"
                     /locus_tag="ACHOS5_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP9775074.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(19885..20769)
                     /gene="tsx"
                     /locus_tag="ACHOS5_000179"
     CDS             complement(19885..20769)
                     /gene="tsx"
                     /locus_tag="ACHOS5_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9775075.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVHSTGWGGYLVVGYNF"
     gene            complement(21068..21607)
                     /gene="yajI"
                     /locus_tag="ACHOS5_000180"
     CDS             complement(21068..21607)
                     /gene="yajI"
                     /locus_tag="ACHOS5_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9775076.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            21758..22207
                     /gene="nrdR"
                     /locus_tag="ACHOS5_000181"
     CDS             21758..22207
                     /gene="nrdR"
                     /locus_tag="ACHOS5_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9775077.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            22211..23314
                     /gene="ribD"
                     /locus_tag="ACHOS5_000182"
     CDS             22211..23314
                     /gene="ribD"
                     /locus_tag="ACHOS5_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP9775078.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALVAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHRIVQQPGETWFARTQDDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            23403..23873
                     /gene="ribE"
                     /locus_tag="ACHOS5_000183"
     CDS             23403..23873
                     /gene="ribE"
                     /locus_tag="ACHOS5_000183"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP9775079.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            23893..24312
                     /gene="nusB"
                     /locus_tag="ACHOS5_000184"
     CDS             23893..24312
                     /gene="nusB"
                     /locus_tag="ACHOS5_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9775080.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            24390..25367
                     /gene="thiL"
                     /locus_tag="ACHOS5_000185"
     CDS             24390..25367
                     /gene="thiL"
                     /locus_tag="ACHOS5_000185"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP9775081.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSNSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            25345..25863
                     /gene="pgpA"
                     /locus_tag="ACHOS5_000186"
     CDS             25345..25863
                     /gene="pgpA"
                     /locus_tag="ACHOS5_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9775082.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(25917..26891)
                     /gene="yajO"
                     /locus_tag="ACHOS5_000187"
     CDS             complement(25917..26891)
                     /gene="yajO"
                     /locus_tag="ACHOS5_000187"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP9775083.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQIDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(26946..28808)
                     /gene="dxs"
                     /locus_tag="ACHOS5_000188"
     CDS             complement(26946..28808)
                     /gene="dxs"
                     /locus_tag="ACHOS5_000188"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9775084.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELSLDATGMEAKIKAWLA"
     gene            complement(28833..29732)
                     /gene="ispA"
                     /locus_tag="ACHOS5_000189"
     CDS             complement(28833..29732)
                     /gene="ispA"
                     /locus_tag="ACHOS5_000189"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP9775085.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHIPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            complement(29732..29974)
                     /gene="xseB"
                     /locus_tag="ACHOS5_000190"
     CDS             complement(29732..29974)
                     /gene="xseB"
                     /locus_tag="ACHOS5_000190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9775086.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            30180..31628
                     /gene="thiI"
                     /locus_tag="ACHOS5_000191"
     CDS             30180..31628
                     /gene="thiI"
                     /locus_tag="ACHOS5_000191"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9775087.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLRVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(31682..32272)
                     /gene="yajL"
                     /locus_tag="ACHOS5_000192"
     CDS             complement(31682..32272)
                     /gene="yajL"
                     /locus_tag="ACHOS5_000192"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP9775088.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(32235..33146)
                     /gene="panE"
                     /locus_tag="ACHOS5_000193"
     CDS             complement(32235..33146)
                     /gene="panE"
                     /locus_tag="ACHOS5_000193"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9775089.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            33314..33805
                     /gene="yajQ"
                     /locus_tag="ACHOS5_000194"
     CDS             33314..33805
                     /gene="yajQ"
                     /locus_tag="ACHOS5_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP9775090.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(33933..35297)
                     /gene="yajR"
                     /locus_tag="ACHOS5_000195"
     CDS             complement(33933..35297)
                     /gene="yajR"
                     /locus_tag="ACHOS5_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9775091.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGLPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(35446..36336)
                     /gene="cyoE"
                     /locus_tag="ACHOS5_000196"
     CDS             complement(35446..36336)
                     /gene="cyoE"
                     /locus_tag="ACHOS5_000196"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP9775092.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(36348..36677)
                     /gene="cyoD"
                     /locus_tag="ACHOS5_000197"
     CDS             complement(36348..36677)
                     /gene="cyoD"
                     /locus_tag="ACHOS5_000197"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP9775093.1"
                     /translation="MSHSNASSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(36677..37291)
                     /gene="cyoC"
                     /locus_tag="ACHOS5_000198"
     CDS             complement(36677..37291)
                     /gene="cyoC"
                     /locus_tag="ACHOS5_000198"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP9775094.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(37281..39272)
                     /gene="cyoB"
                     /locus_tag="ACHOS5_000199"
     CDS             complement(37281..39272)
                     /gene="cyoB"
                     /locus_tag="ACHOS5_000199"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP9775095.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(39294..40241)
                     /gene="cyoA"
                     /locus_tag="ACHOS5_000200"
     CDS             complement(39294..40241)
                     /gene="cyoA"
                     /locus_tag="ACHOS5_000200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP9775096.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(40700..42175)
                     /gene="ampG"
                     /locus_tag="ACHOS5_000201"
     CDS             complement(40700..42175)
                     /gene="ampG"
                     /locus_tag="ACHOS5_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP9775097.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNGNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(42219..42797)
                     /gene="yajG"
                     /locus_tag="ACHOS5_000202"
     CDS             complement(42219..42797)
                     /gene="yajG"
                     /locus_tag="ACHOS5_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9775098.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAKNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            43102..43419
                     /gene="bolA"
                     /locus_tag="ACHOS5_000203"
     CDS             43102..43419
                     /gene="bolA"
                     /locus_tag="ACHOS5_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP9775099.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            43763..45061
                     /gene="tig"
                     /locus_tag="ACHOS5_000204"
     CDS             43763..45061
                     /gene="tig"
                     /locus_tag="ACHOS5_000204"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9775100.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            45307..45930
                     /gene="clpP"
                     /locus_tag="ACHOS5_000205"
     CDS             45307..45930
                     /gene="clpP"
                     /locus_tag="ACHOS5_000205"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9775101.1"
                     /translation="MSYSGERDNFASHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            46056..47330
                     /gene="clpX"
                     /locus_tag="ACHOS5_000206"
     CDS             46056..47330
                     /gene="clpX"
                     /locus_tag="ACHOS5_000206"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP9775102.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            47518..49872
                     /gene="lon"
                     /locus_tag="ACHOS5_000207"
     CDS             47518..49872
                     /gene="lon"
                     /locus_tag="ACHOS5_000207"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP9775103.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            50063..50353
                     /gene="hupB"
                     /locus_tag="ACHOS5_000208"
     CDS             50063..50353
                     /gene="hupB"
                     /locus_tag="ACHOS5_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP9775104.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            50545..52416
                     /gene="ppiD"
                     /locus_tag="ACHOS5_000209"
     CDS             50545..52416
                     /gene="ppiD"
                     /locus_tag="ACHOS5_000209"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9775105.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            52567..52938
                     /gene="ybaV"
                     /locus_tag="ACHOS5_000210"
     CDS             52567..52938
                     /gene="ybaV"
                     /locus_tag="ACHOS5_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9775106.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            53044..53442
                     /gene="fadM"
                     /locus_tag="ACHOS5_000211"
     CDS             53044..53442
                     /gene="fadM"
                     /locus_tag="ACHOS5_000211"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP9775107.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(53494..54189)
                     /gene="queC"
                     /locus_tag="ACHOS5_000212"
     CDS             complement(53494..54189)
                     /gene="queC"
                     /locus_tag="ACHOS5_000212"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP9775108.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(54254..55954)
                     /gene="ybaE"
                     /locus_tag="ACHOS5_000213"
     CDS             complement(54254..55954)
                     /gene="ybaE"
                     /locus_tag="ACHOS5_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP9775109.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKPQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDMGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            56054..56872
                     /gene="cof"
                     /locus_tag="ACHOS5_000214"
     CDS             56054..56872
                     /gene="cof"
                     /locus_tag="ACHOS5_000214"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP9775110.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            57025..57483
                     /gene="decR"
                     /locus_tag="ACHOS5_000215"
     CDS             57025..57483
                     /gene="decR"
                     /locus_tag="ACHOS5_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP9775111.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            57513..59285
                     /gene="mdlA"
                     /locus_tag="ACHOS5_000216"
     CDS             57513..59285
                     /gene="mdlA"
                     /locus_tag="ACHOS5_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9775112.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFIMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            59278..61059
                     /gene="mdlB"
                     /locus_tag="ACHOS5_000217"
     CDS             59278..61059
                     /gene="mdlB"
                     /locus_tag="ACHOS5_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9775113.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            61240..61578
                     /gene="glnK"
                     /locus_tag="ACHOS5_000218"
     CDS             61240..61578
                     /gene="glnK"
                     /locus_tag="ACHOS5_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9775114.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            61608..62894
                     /gene="amtB"
                     /locus_tag="ACHOS5_000219"
     CDS             61608..62894
                     /gene="amtB"
                     /locus_tag="ACHOS5_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP9775115.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(62943..63803)
                     /gene="tesB"
                     /locus_tag="ACHOS5_000220"
     CDS             complement(62943..63803)
                     /gene="tesB"
                     /locus_tag="ACHOS5_000220"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP9775116.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            64021..64593
                     /gene="ybaY"
                     /locus_tag="ACHOS5_000221"
     CDS             64021..64593
                     /gene="ybaY"
                     /locus_tag="ACHOS5_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP9775117.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(64624..64935)
                     /locus_tag="ACHOS5_000222"
     CDS             complement(64624..64935)
                     /locus_tag="ACHOS5_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP9775118.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            65098..65194
                     /gene="ffs"
                     /locus_tag="ACHOS5_000223"
     ncRNA           65098..65194
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOS5_000223"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            65314..65667
                     /gene="ybaA"
                     /locus_tag="ACHOS5_000224"
     CDS             65314..65667
                     /gene="ybaA"
                     /locus_tag="ACHOS5_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP9775119.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(65709..67259)
                     /gene="pdeB"
                     /locus_tag="ACHOS5_000225"
     CDS             complement(65709..67259)
                     /gene="pdeB"
                     /locus_tag="ACHOS5_000225"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP9775120.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHSQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(67423..67893)
                     /gene="ylaC"
                     /locus_tag="ACHOS5_000226"
     CDS             complement(67423..67893)
                     /gene="ylaC"
                     /locus_tag="ACHOS5_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP9775121.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(68009..68560)
                     /gene="maa"
                     /locus_tag="ACHOS5_000227"
     CDS             complement(68009..68560)
                     /gene="maa"
                     /locus_tag="ACHOS5_000227"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP9775122.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(68733..68951)
                     /gene="hha"
                     /locus_tag="ACHOS5_000228"
     CDS             complement(68733..68951)
                     /gene="hha"
                     /locus_tag="ACHOS5_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP9775123.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(68977..69351)
                     /gene="tomB"
                     /locus_tag="ACHOS5_000229"
     CDS             complement(68977..69351)
                     /gene="tomB"
                     /locus_tag="ACHOS5_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP9775124.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(69896..73045)
                     /gene="acrB"
                     /locus_tag="ACHOS5_000230"
     CDS             complement(69896..73045)
                     /gene="acrB"
                     /locus_tag="ACHOS5_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP9775125.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(73068..74261)
                     /gene="acrA"
                     /locus_tag="ACHOS5_000231"
     CDS             complement(73068..74261)
                     /gene="acrA"
                     /locus_tag="ACHOS5_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP9775126.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETSRINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            74403..75050
                     /gene="acrR"
                     /locus_tag="ACHOS5_000232"
     CDS             74403..75050
                     /gene="acrR"
                     /locus_tag="ACHOS5_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP9775127.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            75178..78540
                     /gene="mscK"
                     /locus_tag="ACHOS5_000233"
     CDS             75178..78540
                     /gene="mscK"
                     /locus_tag="ACHOS5_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP9775128.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATQNGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            78601..78699
                     /locus_tag="ACHOS5_000234"
                     /pseudo
     CDS             78601..78699
                     /locus_tag="ACHOS5_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(78752..78913)
                     /gene="rsmS"
                     /locus_tag="ACHOS5_000235"
     CDS             complement(78752..78913)
                     /gene="rsmS"
                     /locus_tag="ACHOS5_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP9775129.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(78927..79454)
                     /gene="priC"
                     /locus_tag="ACHOS5_000236"
     CDS             complement(78927..79454)
                     /gene="priC"
                     /locus_tag="ACHOS5_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP9775130.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            79524..79901
                     /gene="ybaN"
                     /locus_tag="ACHOS5_000237"
     CDS             79524..79901
                     /gene="ybaN"
                     /locus_tag="ACHOS5_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP9775131.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            80054..80605
                     /gene="apt"
                     /locus_tag="ACHOS5_000238"
     CDS             80054..80605
                     /gene="apt"
                     /locus_tag="ACHOS5_000238"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9775132.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            80734..82665
                     /gene="dnaX"
                     /locus_tag="ACHOS5_000239"
     CDS             80734..82665
                     /gene="dnaX"
                     /locus_tag="ACHOS5_000239"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9775133.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    81998..82062
                     /gene="dnaX"
                     /locus_tag="ACHOS5_000239"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            82718..83047
                     /gene="ybaB"
                     /locus_tag="ACHOS5_000240"
     CDS             82718..83047
                     /gene="ybaB"
                     /locus_tag="ACHOS5_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9775134.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            83047..83652
                     /gene="recR"
                     /locus_tag="ACHOS5_000241"
     CDS             83047..83652
                     /gene="recR"
                     /locus_tag="ACHOS5_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9775135.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRKENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            83762..85636
                     /gene="htpG"
                     /locus_tag="ACHOS5_000242"
     CDS             83762..85636
                     /gene="htpG"
                     /locus_tag="ACHOS5_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP9775136.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
BASE COUNT        20714 a        21516 c        22854 g        20565 t
ORIGIN      
        1 taatgattta cagcggcaag ttttacttca aatgaatgtt tcataaactg caccttcaga
       61 tgtcagatta tgtccaacat ttggggtgca gttcatggcg tgtcttatca ggcctacaaa
      121 atgaacagtc ccgtaggccg gataaggcgt tcacgccgca tccggcaaca agcgatgcgc
      181 actgcgactg ctatctaccc cagtggtttc gccagcaccg aactatccgc cgcttgctgg
      241 cgcttatagc gaccatacag cagcaacgac gacatcgcag caaacgaaag cagggcagcg
      301 ggcagcgcca cgtaattata tgccagcccc agcgtcagca tcataccacc gcaatatgcg
      361 ccgacggcgc taccgaggtt aaacgctatt tgcccacctg cggcacctaa taactctccg
      421 cctttggcat tttgcagtaa taatatttgt agaggtgctg atagggcaaa taatcccgcg
      481 caacaaataa aagcaaaaat aagcgacgtt gttttcatac cgccgaagaa aaagagcatc
      541 agcagtgcca gtacaatgat aaagtcagtc actgctgcaa tgcgcagtgg tgaataacgt
      601 cctgaaatcc tgccacttag catattcccc agcaccatcc ctagcccaac taacatcata
      661 ataaaggtca tcgccgtttc cgaaaaaacg gaaataaaca tcatgtatgg ctttacgtag
      721 ctgaaccagg caaacacacc tgcgttgcca aacatcgtag cggcgaaaat taaccacggg
      781 gcagggctgc gtaaaaaatg aaattgttcg cgtagttttc ctttcgcctc gtcgcgaata
      841 tccggcaccc aaaaatagac cgatgccatc accgcaatat taaaaatagc gatcaacaaa
      901 aaggtgtaac gccaactaaa ttcctgactt aaatacgttc ccagtggaat gcccagcaaa
      961 ttggcgactg tcatcccgga aaccatccct gccacggcgg cggtgacttt gccgggtttg
     1021 ataatttttg ataacacgat cgcgccgacg ccaaaaaatg caccatgcgg aaagccggaa
     1081 accagccgac caatggcgag catcaggtaa gacgaagaga gcgtgaacat ggcgttgcca
     1141 atgacgcaca acgccaccag aaacaacaag atatgtttga gtgagtagcg gctggaaaaa
     1201 agcgcgatta taggcgcacc gaccaccacc cccagcgcat aatacgagat catatgcccg
     1261 gcggcaggaa tcgaaattcc tacgttatgc gctagctccg tgagcacgcc cataatgcca
     1321 aattcggcca tccccaaacc aaacgtgccc agagccaaag ataaaataac ttttttcata
     1381 ccaccatcac aaccaccaga acggctacca atcaccgtag ccaataaagt gatagattgc
     1441 aggataacga ccagcgaggc caagagataa ttcgcccctc tgtattcatt atcctgctga
     1501 atagttattt cattgcaaac gcactttcca gtttgctgta gcccagtcca ttgatcttct
     1561 tcactttgat ctgcaccgga atacgctctt tcatcgcctc aacgtggcta atcaccccga
     1621 tggttttgcc actggcattc agggcatcca gcgcatcaag ggcggtatcc agcgtttcgc
     1681 tatccagtgt gccaaaacct tcatcaagga acagcgagtc aatacgcgtt ttatggctga
     1741 ccagatctga aagcgccagt gccagcgcca gactgacgag gaaactttcg ccgccggaaa
     1801 gggtacgggt atcgcgtacc gcatcagcct gccaggtatc aacaacctca acttccagcg
     1861 cttcgctggc tttgcgctgt aacagatagc gcccgtgtag ccgggtgagt tgctgattag
     1921 cgagatggac taaattatcc agcgtcagcc cttgggcaaa cttgcggaat ttatcgccct
     1981 ctttggaacc tattagcgaa ttcagatatc cccagtcctc aacctgctgc gtcatttgag
     2041 caatttgctg cattaaggtt tgttgttgct gacggttatc tgcatcctgc ttcagttgct
     2101 ggcgaatctc gccttgactc gtggtgtttt cacgcaactt ttggtgagtt tgcgctaact
     2161 cttgctgaat ctgctccacc gtcacagtga gagacaaccc gccaggtcgg tgttgctgat
     2221 gctgtgtcag cgtttctgct gtctgagtga ccagcgtttg cgcctgacgg cgctggtttt
     2281 ccagattctg cttgagctgt tccagctgcg ttagtgtttg ttcatccatt agcgccgcaa
     2341 ggaacgcctg ctgatcgtca aagacgctcg cctgtagcgc ggtgtcaaac tgcgcctggg
     2401 ctttttgcag actttgcgcc gccagaacat cctgttgctg taacgtctgc tgctggctgt
     2461 gtaatgcgag acattgttca tgaacctgcc gccagttttc cagcgccaca gtttcttcgc
     2521 tgtgcgggag ttcatcactt tgcggcaacg tttccagaat cggcgtcagt tgctgaatac
     2581 ggttttgcag cgcggttaat tcgttctggc gatgctgcca gctctgcgct tcttgctgac
     2641 gcgtcgccaa ccagctctct tcttcatctt cctgtggcaa tgtcagtgca taacccgcca
     2701 atgccgttaa gagctgctgc tggcgttgtt caatttgctg ttgatactgg ataatttgct
     2761 gattatgcgc ggcaatctgc ccttgtaatt catgccgttg gctgagtaac cgcagctggc
     2821 gttcgtgctc atcttgtgca tctagccacg gttgaatatc gtcctgtggc tgcaaggtga
     2881 tattgaggct ggccgtgacg gcttgccatt gttgagtaag tgcttgctca tcttgccgga
     2941 ggctttgcgc ttcgttttca tcgcgctgaa gctgcttcgt taatgcatcc agttgcccac
     3001 gtagcgccgc accttcttcg ccgagctttt tcacttcttt ttccagcgtt aatagccggg
     3061 cctgattaac gcccggttcc agcgcctgat acgcctcgac cgccgggtgg ctgctggaac
     3121 cacaaagtgg acaaggctga cccgcctgta actgtgcgcg ctgggcttcc agcgttttaa
     3181 tgcgcgcttc ctgctcgcaa atggttttca catcggcaag ttgttgcgtt ttttctttat
     3241 aacgatggcg catttcgtta agtgcggcat tacgttgcgt ctgttccaga gtgacattct
     3301 ggatagcgac ctgtaactgc gccagacgtt tttgttgggg aacaatctgc ccatgcagtg
     3361 cgaccaggcg ctgacgcagt gggcgttgct cagcatgttg tgctagggcg gtagcaactt
     3421 catcggcggt taacgtcaac gtgatcgccg caagcgcatt aagtttttgc tcagcatgag
     3481 ttaactgttg ctgccattgc cgcagatgct cgcgatcgct ggtttgttgg gagaactgtg
     3541 cacgccaacc cgccagttcg ttgttccact gacggaagcg gtcgtgttcc tgtaaccagg
     3601 cattcaggct ttgttgctgc tgctgtaatt ctgctgactg cttcgccgcg tggtggcgaa
     3661 tgctcgcacg aagcgccatt gtgctctgta agcgagtatt tacttcttca atctgctggc
     3721 gagtatgcgc cagcgccgtg ctgtgttctg cgatgcgttc ccagtgcgga cgaagatttc
     3781 gtgccggttg tgccagactg agcgccgcca gttgaggttg cgctttttct tcttcggcta
     3841 atgcctgttg caaggcctgt tgacggcggc tggcttcttg ctgcaattcg tccagacgcg
     3901 ttaaccagtt tagcgattgt tgttcttgct gctgcgcggt aattaactgt ttttcttcgt
     3961 cagtaagtac ctgcaaactc gctgtcagcg attgcacttg ttccggcgtg agcaacgcga
     4021 cgccgctggc ctgcgcttgc agcttctcca gctctgtgcg ggccgatttg tgctgctcaa
     4081 aaaccatcgc cgagatttgc ccgtagattt cagtgccggt taactcctcg agcaattctg
     4141 cgcgttcttt gggtttggca ttcaggaagg cagcaaattg cccctgcgaa agcagcatcg
     4201 aacgggtgaa gcgcccgtaa tccagtccgg ttaacgtcgc cgtcagttcc agcttatctt
     4261 tcactttgtc ggcgagaatt ttgccgtctg cacagcgcgc cagctctacg cgtggcacct
     4321 gcaaattacc gtccggctgg ttacgcgccc gattctggct ccagaacgcg cgatacgctt
     4381 cacctttgac ttcaaactcc acttccgcca gacattcggc ggtatcgcgg gtcatgagat
     4441 cattttgcga ttgtgaaacg ttagagaggc gcggggtttc gtgatacagc gccagacaaa
     4501 tggcgtccag cagggtggtt ttccccgcac cggttgggcc ggtaatagca aacagcccgt
     4561 tgctggcgaa cggctcgcgg gtgaaatcaa tcttccattc gccttttaat gagttcaggt
     4621 ttttcaggcg caggctgaga attttcatgc ttcgtgttct ccggcgaggc tatgcaacgt
     4681 cgtggcgaaa agatgctgca gacgttgctg ctgtgattca tccagttctt ccagtgccag
     4741 acggcgattg aacacctctt cgacgctgag ttcgctgagg gtttcacgct gttggctggc
     4801 taacacacgc tcgcgctgtt cacgactccg gcgtaccagc aatacttcga caggcaatga
     4861 ttcggttaat gcctggattt tgcgctgaat atcatgcaga tactcatcag tggtgatttc
     4921 gatatccagc cagacaggcg gctcctgtga tatatcgcgc cactgttcca gctgtgcggt
     4981 aatcgacgcc agatcgcctt tcagcaccgc catgggttgc gttaccggta cgttcaggtt
     5041 ttccacgctc tctaatttgc cgtttgaaaa tgtcaccaga tggacatatt tactcttacc
     5101 gcattcatca aaactcagtg gtatggggga accgcaatag cgaacatgtt ccatgccgcc
     5161 aataatctgc gcgcggtgaa tatgcccgag cgcgatgtag tcggctggtg gaaagttttg
     5221 tgccggaaac gcgtccagcg tgccaatata aatgtcacgc acggcgtcac ttttactggc
     5281 acccacggtc gttaaatgtc cagtggcgat gatgggcaga ggctgatcgc cgcgcagttt
     5341 gcaggcatcg gcataatgtt gttgataata atcagtaata gccgccagca aatgctgctg
     5401 tttttcaata ccgttaagcc ccgcctggct ggtaataatg tcacgcgggc gtaaaaaagg
     5461 aatgggacac agcactgcgc ctggcgtccc gtcgcgacgg ggcaatattt gcggcgcatg
     5521 tccggcgctg gcgaccacgg tagtattgag gaacgccatg atatcgcgcg attcattcag
     5581 cgtggcgacc gagtcatggt ttcctgccag taccaccaga tgacagcccg tttgctgtaa
     5641 attgacaaca aaacggttgt ataacgtgcg ggcgtaactg ggcggcgagc cggtatcgaa
     5701 aacatcaccg gcaacaataa tcgcatccac ctgatgggct tgtgctgtct ccagcagcca
     5761 gtcaagaaaa gcctgatgtt cggcttcgcg gcttttactg tagaagttct ggccgagatg
     5821 ccagtctgag gtgtgaagga tgcgcataac ggttccctgg cgaaaaagca tgggcgcgat
     5881 tatacccaaa cagatgtgcc atttgctttt ttctgcgcca cggaaatcaa taacctgaag
     5941 atatgtgcga cgagcttttc ataaatctgt cataaatctg acgcataatg acgtcgcatt
     6001 aatgatcgca acctatttat tacaacaggg caaatcatgg cgagacgtat tctggtcgta
     6061 gaagatgaag ctccaattcg cgaaatggtc tgcttcgtgc tcgaacaaaa tggctttcag
     6121 ccggtcgaag cggaagatta tgacagtgct gtgaatcaac tgaatgaacc ctggccggat
     6181 ttaattctcc ttgactggat gttacctggc ggctccggta tccagttcat caaacacctc
     6241 aagcgcgagt cgatgacccg ggatattcca gtggtgatgt tgaccgccag aggggaagaa
     6301 gaagatcgcg tgcgcggcct tgaaaccggt gcggatgact atatcaccaa gccgttttct
     6361 ccgaaggagc tggtggcgcg aatcaaagcg gtaatgcgcc gtatttcgcc aatggcggtg
     6421 gaggaggtga tcgagatgca ggggctaagt ctggacccga catctcaccg ggtgatggcg
     6481 ggcgaagaac cgctggagat ggggccgaca gaatttaaac tgctgcactt ttttatgacg
     6541 catcctgagc gcgtgtacag ccgcgagcag ttgttaaacc acgtctgggg aaccaacgtg
     6601 tatgtggaag atcgtacggt cgatgtccac attcgtcgcc tgcgtaaagc actggagccc
     6661 ggcgggcatg accgcatggt gcagaccgtg cgcggtacag ggtatcgttt ttcaacccgc
     6721 ttttaaggcc ttgctcatcg gacgtagagc agggcttatg atttcttaac tggagtatct
     6781 tacgtgctgg aacggctgtc gtggaaaagg ctggtgctgg agctgctact ttgctgcctc
     6841 ccggctttca tcctgggtgc attttttggt tacctgccct ggtttttgct ggcatcggta
     6901 acaggactgc ttatctggca tttctggaat ttattgcgcc tttcatggtg gctgtgggtg
     6961 gatcgtagta tgaccccgcc accggggcgt ggtagctggg aaccgctact atacggctta
     7021 caccagatgc agctgcgaaa taaaaaacgc cgccgtgaac tgggcaatct gattaaacgc
     7081 tttcgtagcg gcgcggagtc gctgcccgac gcggtggtgc tgaccacgga agagggcggt
     7141 attttctggt gcaacggtct ggcgcaacag attcttggct tgcgctggcc ggaagataac
     7201 gggcagaaca tccttaactt gctgcgttac ccggagttta cgcaatatct gaaaacgcgt
     7261 gatttctctc gcccgctcaa tctggtgctc aacaccgggc ggcatctgga gattcgcgtc
     7321 atgccttata cccacaaaca gttgctgatg gtggcgcgag atgtcacgca aatgcatcaa
     7381 ctggaagggg cgcggcgtaa cttttttgcc aatgtgagcc atgagttacg tacgccattg
     7441 accgtgttac agggttacct ggagatgatg gatgagcaac cgctggaagg cgcggtacgt
     7501 gaaaaagcgt tgcacaccat gcgcgagcag acccagcgga tggaagggtt ggtgaagcaa
     7561 ttgctgacgc tgtcgaaaat agaagccgca ccgacgcatt tgctcaatga aaaggttgat
     7621 gtgccgatga tgctgcgcgt cgttgagcgc gaggctcaga ctctgagtca gaaaaaacag
     7681 acatttacct ttgagataga taacggcctc aaggtgtcag gcaacgaaga tcagctacgc
     7741 agtgcgattt cgaacctggt ctataacgcc gtgaatcata cgccggaagg cacgcatatc
     7801 accgtacgct ggcagcgagt gccgcacggt gccgaattta gtgttgaaga taacggaccg
     7861 ggcattgcac cggagcatat tccgcgcctg accgagcgtt tttatcgcgt tgataaagcg
     7921 cgttcccggc aaaccggtgg tagcggatta gggttagcga tcgtgaaaca tgcggtgaat
     7981 catcacgaaa gtcgcctgaa tattgagagt acagtaggaa aaggtacacg tttcagtttt
     8041 gttatcccgg aacgtttaat tgccaaaaac agcgattaat tcgcctttgt catcttttat
     8101 tgccataagc cagccgatgc tggcttattt tctttgcagt cgaaatacgg gcgttagatt
     8161 ttacaacggt tggtgatttt ttgttcgcat gattagccat gtctttttca ttgaaatagt
     8221 gttttatact ggttggtgat ttcttatcgc tatatacctc tggtttttag atcccttctt
     8281 gctttaaaac gttataagcg tttaaattgc gcttcaggtg ctgtcatact gactgcattt
     8341 acgcggtaaa tcgaaaaact attcttcgcc gcgcctggtt gggagtattt cccgctaaaa
     8401 ttgtttaaat ataccgctgt atcatcccca gggattggca caaaaattta acgttacaac
     8461 accacatcca caggcagtat gatttatgac ccatcaatta agatcgcgcg atatcatcgc
     8521 tctgggcttt atgacatttg cgttgttcgt cggcgcaggt aacattattt tccctccaat
     8581 ggtcggcttg caggcaggcg aacacgtctg gactgcggca ttcggcttcc tcattactgc
     8641 cgttggccta ccggtattaa cggtagtggc gctggcaaaa gttggcggcg gtgttgacag
     8701 tctcagcacg ccaattggta aagtcgctgg cgtactgctg gcaacagttt gttacctggc
     8761 ggtggggccg cttttcgcta cgccgcgtac agctaccgtt tcttttgaag tgggcattgc
     8821 gccgctgacg ggtgattccg cgctgccgct gtttatttac agcctggtct atttcgctat
     8881 cgttattctg gtttcgctct atccgggcaa gctgctggat accgtgggca acttccttgc
     8941 gccgctgaaa attatcgcgc tggtcatcct gtctgttgcc gcaattatct ggccggcggg
     9001 ttctatcagc acggcgactg aggcttatca aaacgctgcg ttttctaacg gcttcgtcaa
     9061 cggctatctg accatggata cgctgggcgc aatggtgttt ggtatcgtta ttgttaacgc
     9121 ggcgcgttct cgtggcgtta ccgaagcgcg tctgctgacc cgttataccg tctgggctgg
     9181 cctgatggcg ggtgttggtc tgactctgct gtacctggcg ctgttccgtc tgggttcaga
     9241 cagcgcgtcg ctggtcgatc agtctgcaaa cggtgcggcg atcctgcatg cttacgttca
     9301 gcataccttt ggcggcggcg gtagcttcct gctggcggcg ttaatcttca tcgcctgtct
     9361 ggtcacggcg gttggcctga cctgtgcttg tgcagaattc ttcgcccagt acgtaccgct
     9421 ctcttatcgt acgctggtgt ttatcctcgg cggcttctcg atggtggtgt ctaacctcgg
     9481 cttgagccag ctgattcaga tctctgtacc ggtgctgacc gccatttatc cgccgtgtat
     9541 cgcactggtt gtattaagtt ttacacgctc atggtggcat aattcgtccc gcgtgattgc
     9601 tccgccgatg tttatcagcc tgctttttgg tattctcgac gggatcaagg catctgcatt
     9661 cagcgatatc ttaccgtcct gggcgcagcg tttaccgctg gccgaacaag gtctggcgtg
     9721 gttaatgcca acagtggtga tggtggttct ggccattatc tgggatcgtg cggcaggtcg
     9781 tcaggtgacc tccagcgctc actaaatcac tgaacatttg ttttaaccac ggggctgcga
     9841 tgccccgtgg ttttttattg tgttgatggg ttaggaattg atggaaagta agaacaagct
     9901 aaagcgtggg ctaagtaccc gacacatacg ctttatggca ctgggttcag caattggcac
     9961 cgggctgttt tacggttcgg cagatgccat caaaatggcc ggtccaagcg tgttgttggc
    10021 ctatattatc ggtggtatcg cggcgtatat cattatgcgt gcgctggggg aaatgtcggt
    10081 acataacccg gcagccagct ctttctcgcg ttatgcgcag gaaaacctcg gcccgctggc
    10141 aggttacatt accggctgga cctactgctt tgaaatcctt attgtcgcta tcgccgatgt
    10201 gaccgctttt ggtatctata tgggtgtctg gttcccgacg gtgccgcact ggatttgggt
    10261 actgagtgtg gtgctgatca tttgcgccgt aaacctgatg agcgtgaagg tattcggtga
    10321 gctggagttc tggttctcgt tctttaaagt cgccactatc atcatcatga ttgtcgccgg
    10381 tttcggcatc atcatctggg ggattggcaa cggcgggcaa ccgaccggta ttcataacct
    10441 gtggagcaac ggcggcttct tcagtaacgg ctggcttggt atggtgatgt cgttgcaaat
    10501 ggtgatgttt gcttacggtg ggatcgaaat tatcgggatt accgccggtg aagcgaaaga
    10561 tcctgagaaa tcgattccgc gtgcgattaa ctccgtgccg atgcgtattc tggtgttcta
    10621 cgtcggtacg ctgttcgtca ttatgtctat ctacccgtgg aatcaggttg gcactgccgg
    10681 tagcccgttt gtgctgacgt tccagcatat gggcattacc tttgccgcca gcattcttaa
    10741 ctttgttgtg ctgaccgctt cgctgtcggc aattaacagt gacgtatttg gtgtaggccg
    10801 tatgctccac ggtatggcag agcagggcag cgcgccaaaa attttcagca aaacctcgcg
    10861 tcgtggtatt ccgtgggtta cggtgctggt gatgactacc gcgctgctgt ttgcggtgta
    10921 tctgaactac atcatgccgg aaaacgtctt cctggtgatc gcttcgctgg caaccttcgc
    10981 cacggtgtgg gtgtggatta tgatcctgct gtcgcaaatc gccttccgtc gccgtttgcc
    11041 gccagaagaa gttaaggcgc tgaaatttaa agtgccgggt ggggtagcaa cgaccatcgg
    11101 cggtttgatt ttcctgctct ttattatcgg gttgattggt tatcacccgg atacgcgtat
    11161 ctcgctgtat gtcggtttcg cgtggattgt tgtgctgttg attggctgga tgtttaagcg
    11221 ccgccacgat cgtcagctgg ctgaaaacca gtaatccttg cgttctgtaa gccggttcag
    11281 gaggaaatcc tgatccggct attccgaaag ttatccgccc ccgtcctcct cccccaaata
    11341 tccttcagat gatgagtgat cctgcattag gctatggcaa ggtgatcaga ttttcatcac
    11401 aggggaatta tgatgttaaa ggcatggcat ctgccggtgc ccccatttgt taaacaaagc
    11461 aaagatcaac tgctcattac actgtggctg acgggcgaag acccaccgca gcgcattatg
    11521 ctgcgtacag aacacgataa cgaagaaatg tcagtaccga tgcataagca gcgcagtcag
    11581 ccgcagcctg gcgtcaccgc atggcgggcg gcgatcgagc tctcctgcgg acaacctcgg
    11641 cggcgttata gtttcaaatt gctgtggcac gatcgccagc gttggtttac accgcaaggc
    11701 ttcagccgaa tgccgccagc acgactggag cagtttgcca ttgatgtacc ggatatcggc
    11761 ccacaatggg ctgcggatca gattttttat cagatcttcc ctgatcgttt cgcccgtaat
    11821 cttcctcgtg aagctgaaca ggatcatgtc tattaccatc acgcagccgg acaagagatc
    11881 atattgcgtg actgggatga accggtcacg gcgcaggctg gcggatcaac gttctatggc
    11941 ggcgatctgg acgggataag tgaaaaactg ccgtatctga aaaaacttgg cgtgacggcg
    12001 ctgtatctca atccggtgtt taaagctccc agcgtgcata aatacgatac cgaggattat
    12061 cgccatgtcg atccgcagtt tggcggtgat ggggcgttgc tgcgtttgcg acacaatacg
    12121 cagcagctgg gaatgcgact ggtgctggac ggcgtgttta accacagtgg cgattcccat
    12181 gcctggtttg acaggcacaa tcgtggcacg gggggagctt gtcacaaccc cgaatcaccc
    12241 tggcgtgact ggtactcgtt tagtgatgat ggcacggcgc tcgactggct gggctatgcc
    12301 agcttgccga agctggatta tcagtcggaa agtctggtga atgaaattta tcgcggggaa
    12361 gacagtattg tccgccactg gctgaaagcg ccgtggaata tggatggctg gcggctggat
    12421 gtggtgcata tgctggggga agcgggtggg gcgcgcaata atttgcagca cgttgccggg
    12481 atcaccgaag cggcgaaaga aacccagccg gatgcgtata ttgtcggcga acattttggc
    12541 gatgcacggc aatggttaca ggccgatgtg gaagatgccg ccatgaacta tcgcggcttc
    12601 acattcccgt tgtggggatt tcttgccaac accgatatct cttacgatcc gcagcaaatt
    12661 gacgcccaaa cctgtatggc ctggatggat aattaccgcg cagggctttc tcatcaacaa
    12721 caattacgta tgtttaatca gctcgacagc cacgatactg cgcgatttaa aacgctgctc
    12781 ggtcgggatg ttgcgcgcct gcccctggcg gtggtctggc tgttcacctg gcctggtgta
    12841 ccgtgcattt attacggtga tgaagtggga ctggatggca aaaacgatcc gttttgccgt
    12901 aagccgttcc cctggcaggt ggaaaagcag gattcggcgt tattcgcgct gtaccagcga
    12961 atgattgcgc tgcgtaagaa aagtcaggcg ttgcgtcgtg gcggctgtca ggtgctgtat
    13021 gcggaagata acgtggtggt atttgtccgc atgctgaatc agcagcgtgt actggtggca
    13081 atcaaccgtg gcgaagcctg tgaagtggtg ctacccgcgt caccgttgct taatgtcgcg
    13141 caatggcaac gcaaagaagg tcacgggcaa ctgacggacg ggattctggc tttgcctgcc
    13201 atttcggcta cggtgtggat gaattaacgt cttataacgc cttgcgtgac gcctgctcca
    13261 tcatccgcgg ataaaactgc cagaagcggg tttcgagagc atcgtaatgg gcgtctaaat
    13321 cgtaccagga gtcacgcagg gcatccagac gtgggcgacg gcttgccatg ccgtttaaca
    13381 cgctctggat gaaatccata tcgcgatagc gcaccaacca ctgctctgac cataagtagt
    13441 tgttcagatt gataaagcgt ggcggtgagt ctggaagaat agtcatcact tgctcccggg
    13501 cataacaggt aaattcctgt agcggaaaat cgggcgacag ctgcgaccag tggcgggaca
    13561 gaaagtgatc ccacatcaca tccagcgtaa taggcgcgac acggcgcgtt tcattacgaa
    13621 accactcccg cgcttcgcgg acttctggca gattgtcggt taatacgtcg attcgccgat
    13681 gcatatgaat accagccacg acgtcgggcg gaaaactttc ttcaggattt ccgcgtacga
    13741 aatcagccag taaattgccg gaaagcgagc tttccgcgag atgggctaaa tgcaggtgag
    13801 ctaaaaaatt catcgattat attctatcca aaggggggta aaggttgcag ggagagcgcc
    13861 ccggcactag actacccgcc tcttatttta gtctgagtca gtgtcatgcg cgttaccgat
    13921 ttctcttttg aattgcccga atccctgatt gcccactatc ccatgcctga acgcagtagc
    13981 tgtcgtttac tgtcgctgga cgggccgacg ggcgcgctga cgcacggtac tttcaccgat
    14041 ttactcgata agctcaaccc cggcgatctt ctggttttta ataatacccg cgtgatcccg
    14101 gcgcgtctgt ttgggcgtaa agccagcggc ggcaagattg aagtgctggt tgagcggatg
    14161 ctcgacgaca aacgcattct tgcgcatatt cgcgcctcga aagcgccaaa acctggcgca
    14221 gaactgctgc tgggcgatga cgaaagtatt aacgcaacaa tgaccgcgcg ccacggcgca
    14281 ctgtttgaag tcgaatttaa tgatcaacgt tcggtgctgg atattctcaa cagcatcggc
    14341 catatgccgc tgccgccgta catcgaccgt ccggacgaag atgcagaccg cgaactttat
    14401 caaacggttt atagcgaaaa acccggcgcg gtagcagcac cgaccgccgg actgcacttt
    14461 gacgaacctt tgctggaaaa attacgcgcc aaaggcgtgg agatggcgtt tgtgacgttg
    14521 cacgttggtg cgggcacctt ccagccggtg cgcgtcgaca ccattgaaga tcacatcatg
    14581 cactcggaat acgctgaagt gccgcaggat gtggtagacg cggtactggc ggcgaaagcg
    14641 cgcggtaacc gggtgattgc ggttggtacc acctcagtac gttcgctgga aagtgcggct
    14701 caggcagcga aaaacgatct cattgaaccg ttctttgacg atacccaaat ctttatctat
    14761 ccgggcttcc agtacaaagt ggtcgatgcg ctggtgacga acttccacct tccggaatcg
    14821 acgctgatta tgctggtttc ggcatttgct ggttatcaac acaccatgaa tgcctataaa
    14881 gcggcggtag aagagaaata tcgctttttt agttacggtg atgcgatgtt tatcacgtac
    14941 aatccgcagg caattaatga gcgcgttggg gagtaattcc gcggcgctgg tttaaacgtt
    15001 ggactgtttt tctgacgtag tggagaaaaa atgaaatttg aactggacac caccgacggt
    15061 cgcgcacgcc gtggccgcct ggtctttgat cgtggcgtag tggaaacgcc ttgttttatg
    15121 cctgttggca cttacggcac cgtaaaaggg atgacgccgg aagaagttga agccactggc
    15181 gcgcaaatta tcctcggcaa caccttccac ctgtggctgc gcccgggcca ggaaatcatg
    15241 aaactgcacg gcgatctgca cgattttatg cagtggaaag gaccaatcct taccgactcc
    15301 ggcggcttcc aggtcttcag ccttggtgat attcgtaaaa tcaccgaaca gggcgttcac
    15361 ttccgcaacc cgatcaacgg cgacccgatt ttcctcgacc cggaaaagtc gatggagatt
    15421 cagtacgatc ttggttcgga tatcgtcatg atctttgatg agtgtacgcc gtatcctgct
    15481 gactgggatt acgcaaaacg ctccatggag atgtctctgc gttgggcgaa gcgtagccgt
    15541 gagcgttttg acagtctcgg aaacaaaaat gcgctgtttg gtatcattca gggcagtgtt
    15601 tacgaagatt tacgtgatat ttctgttaaa ggtctggtag atatcggttt tgatggctac
    15661 gctgtcggcg gtctggctgt gggtgagccg aaagcagata tgcaccgcat tctggagcat
    15721 gtatgcccgc aaattccggc agacaaaccg cgttacctga tgggcgttgg taaaccagaa
    15781 gacctggttg agggcgtacg tcgcggtatc gatatgtttg actgcgtaat gccaacccgc
    15841 aacgcccgaa atggtcattt gttcgtgacc gatggcgtgg tgaaaatccg caatgcgaag
    15901 tataagagcg atactggccc actcgatcct gagtgtgatt gctacacctg tcgcaattat
    15961 tcacgcgctt acttgcatca tctcgaccgt tgcaacgaaa tattaggcgc gcgactcaac
    16021 accattcata accttcgtta ctaccagcgt ttgatggcgg gtttacgcaa ggctattgaa
    16081 gagggtaaat tagagagctt cgtaactgat ttttaccagc gtcaggggcg agaagtacca
    16141 cctttgaacg ttgattaata ttaataatga gggaaattta atgagctttt ttatttctga
    16201 tgcggtagcg gcaacgggtg caccggcgca aggtagcccg atgtctttga ttttgatgct
    16261 ggtggtattc ggtctgattt tctatttcat gatcctgcgt ccacagcaga agcgcaccaa
    16321 agaacacaaa aagctgatgg actccatcgc taaaggtgat gaagtgctga cgaacggtgg
    16381 cctggtcggt cgcgtaacca aagtagcgga aaacggctac attgctatcg cactgaatga
    16441 caccactgaa gtagttatta aacgtgactt cgtagctgcc gttctgccga aaggcaccat
    16501 gaaggcgctg taattaaaat ttttccctaa gggaattgcc gtgttaaacc gttatccttt
    16561 gtggaagtac gtcatgctga tcgtggtgat tgtcatcggt ctgctgtatg cgcttcccaa
    16621 cctgtttggt gaggatccgg ctgttcagat cactggtgcg cgcggagtcg ccgccagtga
    16681 gcaaacgctg atccaggtcc agaaaacgtt acaagaagaa aaaataactg ctaagtctgt
    16741 ggcactggaa gagggcgcta ttcttgcgcg cttcgactcc actgacaccc agctgcgcgc
    16801 tcgtgaagca ttaatgggcg ttatgggtga caaatacgtc gtggcgctta accttgcccc
    16861 ggcaacgccg cgctggctgg cagctattca tgctgagccg atgaagctcg gccttgacct
    16921 gcgtggcggc gttcacttcc tgatggaagt ggatatggac accgcgcttg gcaaactcca
    16981 ggaacaaaat atcgatagcc tgcgcagcga cctgcgtgaa aagggcatcc cgtataccac
    17041 cgttcgtaaa gaaaacaact acggcctgag catcactttc cgcgatgcta aagctcgtga
    17101 tgaagccatt gcgtatctga gcaagcgcca tccggacctg gtgattagca gccagggcag
    17161 caaccagctg cgtgcggtaa tgagcgatgc tcgtctgagt gaagcgcgtg aatatgcggt
    17221 gcagcagaac atcaatatcc tgcgtaaccg tgtaaaccaa cttggcgtgg cggagccggt
    17281 ggttcagcgt cagggtgctg accgtatcgt tgttgaactg ccaggtattc aggacactgc
    17341 gcgtgcgaaa gagattctgg gtgcgacggc aacgctggaa ttccgtctgg taaacaccaa
    17401 cgttgatcag gccgctgcgg catccggtcg cgtaccgggc gactctgaag tgaaacagac
    17461 ccgcgaaggt cagccggttg tgctgtacaa acgcgtaatt ctgaccggtg accatatcac
    17521 cgactccact tccagccagg acgaatacaa ccagccgcag gttaacatct cgctcgatag
    17581 cgcgggtggt aacatcatgt ctaacttcac caaggacaac atcggcaaac cgatggcaac
    17641 cctgtttgtg gagtacaaag acagcggtaa gaaagatgca aacggtcgtg cggttctggt
    17701 gaaacaggaa gaggtgatta acatcgccaa catccagtct cgtctgggta acagcttccg
    17761 tatcaccggc atcaacaacc cgaacgaagc gcgtcagctg tcgctgctgc tgcgtgccgg
    17821 tgcgttgatt gcaccgattc agattgttga agaacgtacc attggtccga ccctgggtat
    17881 gcagaacatt gagcaggggc tggaggcttg ccttgccggt ctgctggttt ctattctgtt
    17941 catgatcatc ttctataaga agtttggtct gattgcgacc agtgctctga ttgccaactt
    18001 gatcttgatt gtcggtatta tgtcgctgtt gccaggcgca acgctgagta tgccaggtat
    18061 cgcgggtatc gtcttaaccc ttgcggtagc ggtcgatgcg aacgtactga tcaacgaacg
    18121 tattaaagaa gagttgagca acggacgtac tgttcaacag gcaattgatg aaggttatcg
    18181 tggcgcattc agttccatct tcgatgcgaa catcaccacg ctgattaaag tcatcatcct
    18241 gtatgcagtg ggtaccgggg caattaaagg gttcgcgatt actaccggta tcggtgtggc
    18301 gacgtcgatg tttaccgcga ttgtcggtac gcgtgccatc gtaaacctgc tatatggcgg
    18361 caagcgcgtc aagaagctgt caatctgagg agtgcgatgt ggcacaggaa tatactgttg
    18421 aacaactaaa ccacggccgt aaagtctatg actttatgcg ctgggactac tgggctttcg
    18481 gcatctctgg tctgctgtta atcgccgcta tcgttattat gggcgtgcgc ggctttaact
    18541 gggggctgga ttttaccggt ggtacggtta ttgaaattac gctcgaaaaa ccggctgaaa
    18601 ttgacgtaat gcgtgatgca ttgcaaaaag ccggttttga agagccgatg ctgcaaaact
    18661 ttggtagcag ccacgacatc atggtccgta tgccgcctgc tgaaggcgaa accggcggtc
    18721 aggtgctggg cagccaggtt ctgaaggtga ttaacgaatc caccaatcag aatgcggcag
    18781 tgaagcgtat tgagttcgtc ggtccgagcg tgggggcaga ccttgcgcaa accggtgcga
    18841 tggcgctgat ggcagcgctg ctgtctatcc tcgtgtacgt tggtttccgc tttgagtggc
    18901 gactggcggc aggggttgtt attgcgctgg cgcacgacgt tatcattacg ctgggtattt
    18961 tgtcgttatt ccatatcgag attgacctga ccattgtggc atcattgatg tcggttatcg
    19021 gttactcgct taacgacagt atcgtggtat cggaccgtat tcgtgaaaac ttccgcaaga
    19081 tccgtcgcgg tacgccttac gaaatcttta acgtgtcctt gacccagacg ctgcaccgta
    19141 ccttgatcac atccggtact accttgatgg ttatcctgat gctgtacctc ttcggtggtc
    19201 cggtactgga aggcttctcg ctgaccatgc ttatcggtgt ttccatcggt actgcatctt
    19261 ccatctatgt ggcatctgcg ctggctctga aactgggtat gaagcgcgaa cacatgttgc
    19321 agcagaaagt ggaaaaagaa ggggcggatc agccgtcaat tctgccttaa tcaagtttca
    19381 gctgatgttg aaaatcccgg tcagaagatc gggatttttt ttgatgtaag gtgattgcga
    19441 aaatccccgc atcttgggaa actgcgcgta accctacatt tcatccaggt aactttttca
    19501 tggctatcat cccaaaaaac tatgcgcggt tagaaagcgg ctatcgcgaa aaagcattaa
    19561 aaatctatcc gtgggtctgc ggtcgctgtt cccgcgagtt tgtttattcc aacctgcgtg
    19621 aacttaccgt tcaccacatt gatcacgacc atactaataa cccggaagat ggcagtaact
    19681 gggaattact ttgtctctat tgccacgatc atgagcattc gaaatacact gaagcggatc
    19741 agtatggtac gaccgttatc gcaggggaag atgcgcagaa agatgtcggt gaagcgaagt
    19801 acaacccatt cgctgatttg aaagcgatga tgaacaagaa gaagtgataa aaaatgccgg
    19861 atatgaaatc cggcattctc acaattagaa gttgtaacct actaccagat aaccacccca
    19921 gccggtagag tgaacgttga agttgccgtt gccgaagttc agttctgcat cgtcattcca
    19981 ctgaccaccg ttatgccagt aacgggcaac gacagagtag tgccagtgat cgtaattcag
    20041 cgccagaata tggctggacg caatagagtt attggtacgt gttttaatac cgttgttcgc
    20101 ataaccgctg tcatcgccca ggtcggaacc ccagtcgaag ttggtgaagc cgatgtagct
    20161 cagctggccg ccccacagat cggtaatcgg cacaaagtac ttcactttga aacggtaacc
    20221 gtcccactcg ttttcgttcg ctgcgccgta gttctgccac tggtatttcg catagacgtt
    20281 catggacagg ctcatcggca gaccagtatc gatgtcggta cccagaccca tgtaccaggt
    20341 gctctggcgg ccatctttat tacgacccat gtcgtaaatg tagttgttcg caaagtacca
    20401 ctctttgaac ggaccgaagc taaggtcagt attggtcagc ttgtcaatgg agaaacgcgg
    20461 ttcgatttcc ataaacagcg gagaaccgtg gttccagata ccttttgcat cggagttacc
    20521 gccgaagaat accggcgcat ccgcataacc atagaagtcg aaccagtctt ttttagcgaa
    20581 tgcttcgtac tcaaggtagg tatcgttgcg gagctgcggt ccgaaacggg tgtgatagct
    20641 tccgacaacg ttaacgctct ggtgccacca gtcggaaaga tactgcggtt tgtcgttttc
    20701 agctgcgttg acagtaaaag acgaagagag cgccagtacc gcaccggctg ccagtaatgt
    20761 ttttttcata tgtatgccac tgtttgaaaa tcccttgcgg gagtgaaaaa ggcgcaaatt
    20821 gcgtttctaa atatttcgta tacagaggag cctattataa aaatcattgc tcacaaaaat
    20881 atgtttcgtt tcacagttct atcatttacg taatcgattg cgttcacgtt tacacacatt
    20941 cgggcgggga ttgtactgac tttcaccctt gttgcaaact tttactatca atccaggtga
    21001 tgtaagcgga gtaacaaaat gacggagaaa gatgttgcgg gacgcacaat atcgccccgc
    21061 aatacgttta ctgcataaca ggttgaatgt cgtggatacg tacaaagcct aactgatcca
    21121 cagaaatacc ctttaactgt aacggaatat caacatcgct gggggcgagg acgctggcag
    21181 gtgcgttaat caattgattc tgcacattga tttcctgaaa gttgtctatt gttccctgaa
    21241 tttgcccata ttcaacggtt ccgctgaatg cgggtaaagg gtcattggat tcgccctgaa
    21301 tacgtagcgt cagggtggtg ccatctgtat caggcgtaat attcaccagc gacatacgta
    21361 aagtaccgat ctggctttcc agtcttgctg gtgtttttgc tccaggcaga aggtaaaccc
    21421 cactgctgga ttttgcattc agcctgtttt gctgagtgat tttgaccgtt tctttattga
    21481 gctgcgtcat ctcctggttc agcgtgctga cgctgtgttt catctgtcgc acttcacttt
    21541 gctgcacaca ggcgctaagg ctgaacaggc ttcccagcag gagcagtcga aaaacgttag
    21601 tattcatagg tcgtcccttt ctcgtcgcaa ttagcctaat ggtagagggt aaaagcagtg
    21661 aaagcatcga tcctttatct caaaagcgtt gcgcctttgt tgtatcgtca gttcagggta
    21721 aaatagattt ctgttaacca cctggtcagg acgccgtatg cattgcccat tctgtttcgc
    21781 cgtggacact aaggtaattg actctcgtct cgtgggcgag ggttcatccg tacgccgccg
    21841 tcggcagtgt ctggtgtgta atgaacgttt caccaccttt gaagtggcgg agctggttat
    21901 gccgcgtgtt gtaaaaagca acgacgtgcg tgaaccgttt aatgaagaga aattacgtag
    21961 cggaatgctg cgggcgctgg aaaaacgtcc ggtgagttcc gatgacgtcg aaatggcaat
    22021 caatcatata aaatcgcagt tgcgcgccac cggtgagcgc gaagtgccga gcaagatgat
    22081 tggcaatctg gtgatggagc aattgaaaaa gctcgataaa gtcgcctata tccgttttgc
    22141 ctctgtctac cgcagtttcg aagatatcaa agaatttggc gaagagatcg cgcgcctgga
    22201 ggactaagcc gtgcaggacg agtattacat ggcgcgggcg ctaaagctgg cgcaacgagg
    22261 acgttttacc acgcatccca acccgaatgt cgggtgcgtc attgtcaaag atggcgaaat
    22321 tgtcggtgaa ggttaccatc aacgcgcggg tgaacctcat gccgaggtac acgcgttgcg
    22381 catggccggc gaaaaagcca aaggcgcaac ggcgtatgtc acactcgaac cctgtagcca
    22441 tcatggtcgt acgccaccgt gttgtgatgc attagttgct gcgggcgtgg cgcgcgtggt
    22501 tgctgcaatg caagacccca atccgcaggt cgctggacgt ggactttacc gtctgcaaca
    22561 ggctggcatt gacgtcagcc acgggctgat gatgagtgaa gctgagcaat tgaataaagg
    22621 ctttctcaag cggatgcgca ccggctttcc ttatattcag ttaaaacttg gcgcatcgct
    22681 tgatggtcgc acggcgatgg cgagtggcga aagccagtgg atcacttcac ctcaggcgcg
    22741 acgcgacgta caacgactgc gcgcgcaaag tcatgccatt ttaaccagca gcgccacggt
    22801 gctggcggat gatccagcct taacagtgcg ttggtctgaa ctggatgaac aaactcaggc
    22861 gctctatccg caacaaaatc tccgtcagcc gatacgtatt gtgattgata gccaaaatcg
    22921 cgtgacgccg gaacatcgca ttgtgcagca gcccggcgaa acctggttcg cgcgtacgca
    22981 ggatgattct cgtgagtggc cggaaacggt gcgtaccttg ctgattccag agcataaagg
    23041 tcatctggat ctggttgtac tgatgatgca actgggtaaa cagcaaatta acagcatctg
    23101 ggtggaagcg gggccaacgc tcgctggcgc attgctacag gcgggtttag tcgatgagct
    23161 gattgtctat atcgcaccta aactattagg caacgacgcc cgtggattat gcacgctgcc
    23221 agggcttgag aaattagccg acgctcccca atttaaattc aaagagatac gtcatgtagg
    23281 cccggatgtt tgcctgcatt tagtgggtgc atgatctccc ggctcgaaag ggaagcagcg
    23341 cacgaaatat tatgctaaaa tccgcccccc tgcggggcca tactcgaacc cgaaggaaga
    23401 aaatgaacat tattgaagct aacgttgcta ccccggacgc tcgcgtcgcc atcaccatcg
    23461 cgcgtttcaa caactttatc aatgacagcc tgctggaagg tgcaattgac gcactgaaac
    23521 gtatcggtca ggtaaaagat gaaaacatta ccgttgtttg ggtgcctggt gcctatgagc
    23581 tgccgctggc ggcgggtgca ctggctaaaa ccggtaaata cgacgcggtg attgcgctgg
    23641 gtacggttat tcgtggtggc actgcccact ttgaatatgt cgctggtggt gcaagcaacg
    23701 gcctggcgca tgttgcccag gacagcgaaa ttccggttgc ttttggggtt ctgaccactg
    23761 aaagcattga acaagcgatc gaacgtgctg gcaccaaagc tggcaacaaa ggtgcagaag
    23821 ctgcactgac cgcgcttgaa atgattaatg tattgaaagc catcaaggcc taaaattagt
    23881 aaggggaaat ccgtgaaacc tgctgctcgt cgccgcgctc gtgagtgtgc cgtccaggcg
    23941 ctctactcct ggcagttgtc ccagaacgac atcgctgatg ttgaatacca gttcctggct
    24001 gaacaggatg taaaagacgt tgacgtcctg tacttccgtg agctgctggc cggggtggcg
    24061 actaacaccg catacctcga cggactgatg aagccgtacc tgtcccgtct gctggaagaa
    24121 ctgggccagg tagaaaaagc agtactgcgc attgcgctgt acgagctgtc taaacgtagc
    24181 gatgtgccat acaaagtggc cattaacgaa gcgatcgaac tggcgaaatc gttcggcgca
    24241 gaagacagcc ataagttcgt caacggcgta ctcgataaag cagcacctgt gattcgccct
    24301 aacaaaaagt aatatccagg ccggtagatt cacggaagac cgttccatga tcgccggcct
    24361 tttctttttt acctgctgag gcataacgta tggcatgtgg cgagttctcc ctgattgccc
    24421 gttattttga ccgtgtaaga agttctcgtc ttgatgtcga actgggcatc ggcgacgatt
    24481 gcgctcttct caatatcccc gagaaacaga ccctggcgat cagcactgat acgctggtgg
    24541 cgggcaacca cttcctccct gatatcgatc ctgctgatct ggcttataaa gcactggcgg
    24601 tgaacctaag cgatctggca gcgatggggg ccgatccggc ctggctgacg ttggcattaa
    24661 ccttaccgga cgtagacgaa gcgtggcttg agtccttcag caacagcttg tttgatcttc
    24721 tcaattatta cgatatgcaa ctcattggcg gcgataccac gcgtgggcca ttatcaatga
    24781 cgttgggtat ccacggcttt gttccgatgg gacgagcctt aacgcgctct ggggcgaaac
    24841 cgggtgactg gatctatgtg accggtacac cgggcgatag cgctgccggg ctggcgattt
    24901 tgcaaaaccg tttgcaggtt gccgatgcta aagatgcgga ctacttgatc aaacgtcatc
    24961 tccgtccgtc gccgcgtatt ttacaggggc aggcactgcg cgatctggca aattcagcta
    25021 tcgatctctc tgacggtctg atttccgatc tcgggcatat cgtgaaagcc agcgactgcg
    25081 gcgcacgtat tgacctggca ttgctgccgt tttctgatgc gctttctcgc catgttgaac
    25141 cggaacaggc gctgcgctgg gcgctctctg gcggtgaaga ttacgagttg tgtttcacgg
    25201 tgccggaact gaaccgtggc gcgctggatg tggctctcgg acacctgggc gtaccgttta
    25261 cctgtatcgg gcaaatgacc gccgatatcg aagggctttg ttttattcgt gacggcgaac
    25321 ctgtcacgtt agactggaaa ggatatgacc attttgccac gccataaaga tgtcgcgaaa
    25381 agtcgcctga aaatgagtaa tccgtggcat ctgcttgctg tcggattcgg aagtggatta
    25441 agcccgatcg ttcctggcac gatggggtcg ctggcggcga ttcccttctg gtatctgatg
    25501 acctttttgc cctggcagct ctattcgctg gtggtaatgc tggggatctg tatcggcgtc
    25561 tatctttgtc atcaaacggc gaaagacatg ggcgtgcacg atcacggcag tattgtctgg
    25621 gacgaattta ttggtatgtg gattacgctc atggcgctgc cgaccaatga ctggcagtgg
    25681 gttaccgccg gatttgtgat tttccgtatt ctggatatgt ggaagccgtg gccgatccgc
    25741 tggtttgatc gcaatgtaca tggcggcatg gggatcatga ttgacgatat tgtcgccggg
    25801 gtgatttccg caggcatcct gtactttatt ggacatcact ggccgctggg tattctgtcg
    25861 tagttgtgca ccgatgcctg atgtgacgct tgtcacgtct catcaggcct gtatacctat
    25921 ttaaatccta cgaccggatg cggtttatac ggcgtttcca gctcggcgat ctgttccggc
    25981 ttcaaggtga tatccaccgc gtttagcagt tcatcaatct gttcctcccg agaagttccg
    26041 ataatcggtg cggcaatgcc cggtttactc aacaaccagg ccagcgcaac ttgtgctcgc
    26101 gttgcaccaa gttcttcact gacgcccgtt aagcgttctg caatttgtgc gtcattttca
    26161 tcgctttctt ggtagagatt tttccctact tcatccgaca ccagccgtgc ggtggtttct
    26221 ccccaaggac gcgtcagacg gccccgcgcc agcgggctcc atggaatcac cgccacgcct
    26281 tcctgataac acagcggcag catctcgcgc tcttcttcac gataaatcag attgtagtga
    26341 tcctgcatac tgacaaactg cgcccagccg tgctgttttt gtagttccag tgcctgagca
    26401 aactgcgaag cgtgcatgga tgacgcgccg atataacgcg ctttcccggc ttttaccacg
    26461 tcgttcaggg cttccagcgt ctcttcgatc ggcgtgttgt aatcccagcg atgaatttgc
    26521 aggatatcga cataatccat gccgagacgg agcaggctgt cgtcgataga gcgcaaaatt
    26581 tgtgcacggg ataatccttc cggtaaatca ccaacgcgat ggaacacttt ggtcgcaacg
    26641 accacgtctt cacgacgggc gaaatcccgc agtgcgcgac cgacgatctc ttcgctgctg
    26701 ccatcggaat agctattggc ggtgtcaaag aaatttatgc cgccttccag cgcacgttta
    26761 atgatgggac ggctgctctc ttccggcagt gtccatgcgt gattaccgcg atctggctcg
    26821 ccaaaggtca tacagccgag gcaaagtcgg gaaacacgaa ggtcggtttt tcctaagggg
    26881 ttgtattgca tgctgccact cctgccatga ataatttctt aagcatagca ggagtagagt
    26941 agggattatg ccagccaggc tttgattttg gcttccatac cggtggcatc gaggctgagt
    27001 tcggcgcgca tttcttcctg agttccttgt ggaataaaga agtccggcag accaatgttc
    27061 agcacgggta ctggtttacg atgggccatc agcacttcgt tcacgccgct gcctgcacca
    27121 cccataatgg cgttttcttc tacggtaacc agcgcttcat ggctggcggc catttccaga
    27181 attaacgctt catcaagcgg tttcacgaaa cgcatatcga ccagcgtagc gttcagcgat
    27241 tcagcgactt tcgccgcttc tggcatcagc gtaccaaagt taaggatcgc cagtttctca
    27301 ccacgacgct tcacaatgcc tttgccaatt ggcagttttt ccagtggcgt cagttccacg
    27361 ccaactgcgt tgccgcgcgg gtagcgcacc gcggacgggc catcgttata gtgatagccg
    27421 gtatagagca tctggcgaca ttcgttttca tcgctcgggg tcataatgac catttccggt
    27481 atgcagcgca ggtaagagag atcaaaagcg ccctggtggg tttgaccgtc agcaccaaca
    27541 atgcccgcac ggtcgatggc gaacaggacc ggaagctttt gaatcgccac gtcatgcagc
    27601 acctgatcat aggcgcgttg caggaaggtg gagtagatcg caacaatggg tttgtagcct
    27661 ccaatcgcca gacccgcagc aaaggtcact gcgtgttgct cggcaattgc cacgtcgaag
    27721 tagcgatccg ggaatttacg tgaaaactcg accatgccgg aaccttcacg catcgccgga
    27781 gtaatcgcca tcagcttgtt atctttcgct gcggtttcgc acaaccagtc gccaaagatt
    27841 tttgaatagc ttggcaaacc gccgctactt ttcggcagac aaccgctgga gggatcaaat
    27901 ttaggcacgg cgtggaaggt gattgggtct ttttctgccg gttcataacc acgacctttt
    27961 ttggtcatga tatgcaggaa ctgcgggcct ttcaggtcgc gcatgttctt aagcgtggtg
    28021 ataagcccca gcacatcgtg accgtccacc gggccgatgt agttaaagcc cagctcttca
    28081 aacaacgtgc caggcactac catgccttta atatgttctt cggtacgttt gagcagctct
    28141 ttaatgggtg gcacgccaga gaaaactttt tttccgcctt cgcgcagtga agagtaaagc
    28201 ttaccggaaa gcagctgcgc cagatggttg ttgagcgcgc caacattttc ggaaatcgac
    28261 atttcattgt cgttgagaac caccagcata tcaggacgga tatcgcccgc gtgattcatc
    28321 gcttcaaacg ccatgccagc agtaatcgcg ccatcgccaa tgacacagac ggtgcggcga
    28381 tttttgcctt ctttctcggc agcaactgcg atcccaattc cggcactgat ggaggttgat
    28441 gaatgcccga cgcttaatac gtcatattcg ctttcgccgc gccacgggaa cgggtgcagg
    28501 ccacctttct gacggatggt gccgattttg tcgcggcgcc cggtcaaaat tttatgcgga
    28561 taagcctgat gccccacatc ccagattaac tggtcaaacg gggtattgta gacatagtgc
    28621 agcgccacgg tcagttcgac cgtgcccagc ccggaggcga agtgcccgct ggaacggctc
    28681 acgctgtcga gtaaatagcg gcgcagttcg tcgcagagtt tcggtaaact ctctttcggc
    28741 aacagtcgta actcctgggt ggagtcgacc agtgccaggg tcgggtattt ggcaatatca
    28801 aaactcatca ggggcctatt aatacttatt gtttatttat tacgctggat gatgtagtcc
    28861 gctagcgctt ccagtgccga ggtatcgagt gactgttcag tcagttgttt cagcgactga
    28921 cgggcatcat cgatcagatc ccgggctttc ttccgggctt gctcaagacc cagaagtgca
    28981 gggtaggtac ttttaccaag ttgctggtcg gcaccctggc gttttcccaa cgttgcagta
    29041 tctcccacca catccaggat gtcatcctga acctggaagg caaggccgat gctctctgcg
    29101 tacttgtcga gtactggcag agcacgacgt cctttatctc cggcgcttaa tgcaccaagg
    29161 cgaacggcgg cgcgaatcaa tgcgccggtt ttatgacgat gaatacgctc aagcgcgtcc
    29221 agaggtatgt gtttgccttc agcgtctaaa tctaatgcct gaccaccgca cattccggca
    29281 ataccgctgg cgctcgccag ctcagaaatc atcgaaattc tgtcgcgatc tgacacttcc
    29341 ggcatatcgg catcgcttag aatcgagaac gccagcgttt gtaaagcgtc accagcgaga
    29401 atcgcgtttg cttcgccaaa cttcacatgg caggtcggca aaccgcgacg cagatcgtca
    29461 tcatccatcg ccggtaaatc atcatgaatt aatgagtaag cgtggataca ctctacggcg
    29521 gcagcgggtg cgtccagtgt gtttgtgcta acgccgaaca tatgaccagt ggcataaacc
    29581 aggaaaggtc gcaggcgctt accacctaat aatgcgccat actgcatggt ttcgaccacg
    29641 ggagtgttct gaaagggcag tggggcgata aaacggctca gcgcctggtt ggcctgctta
    29701 acgcaggctt cgagttgctg cggaaagtcc attactcatt gtccggtgta aaaggggtta
    29761 gagaggcgtc ttcattgtca gacagcagaa tttgtacgcg ctgttcggct tgttgtaatt
    29821 tggcctgccc ctgacgtgcc agctgcacgc cgcgttcgaa ctcgttcagc gcctcttcca
    29881 gcggcaggtc accactttcc agacgggtta caatctgttc cagctcgctc agcgcctttt
    29941 caaagctggc gggcgcctca tttttcttcg gcataatgaa tgtctgactc tcaatatttt
    30001 tcgccccgtc atggtaacgg actcagggca aatagcaaat aacgcgcaat ggtaaggtga
    30061 tgtgcgcagc aaagcgatgt tagtggtata cttccgcgcc tggatgcagc cgcaggtgtg
    30121 ggctgctgta tttttcccta tacaagtcgc ttaaggcttg ccaacgaacc attgccgcca
    30181 tgaagtttat cattaaattg ttcccggaaa tcaccatcaa aagccaatct gtgcgcttgc
    30241 gctttataaa aatccttacc gggaacattc gtaacgtttt aaagcactat gatgagacgc
    30301 tcgctgtcgt ccgccactgg gataacatcg aagttcgcgc aaaagatgaa aaccagcgtc
    30361 tggctattcg cgacgccctg acccgtattc cgggtatcca ccatattctc gaagtcgaag
    30421 acgtgccgtt taccgacatg cacgatattt tcgagaaagc gttggttcaa tatcgcgatc
    30481 agctggaagg caaaaccttt tgcgtacgcg tgaagcgccg cggcaaacat gattttagct
    30541 cgattgatgt ggaacgttac gtcggcggcg gtttaaatca gcatattgaa tccgcgcgcg
    30601 tgaagctgac caatccggaa gtgactgtcc atctggaagt ggaagacgat cgtctgctgc
    30661 tgattaaagg ccgctacgaa ggtattggcg gtttcccgat cggcacccag gaagatgtgt
    30721 tgtcgctcat ttccggtggt ttcgactccg gtgtttccag ttatatgtta atgcgtcgcg
    30781 gctgccgcgt gcattactgc ttctttaatc tcggcggcgc ggcacacgaa attggtgttc
    30841 gtcaggtggc gcattatctg tggaaccgtt ttggtagctc ccaccgcgtg cgttttgtcg
    30901 ccattaactt tgagccggta gtcggtgaaa ttctcgagaa aatcgacgac ggtcagatgg
    30961 gcgtaatcct caaacgtatg atggtgcgtg ccgcgtctaa agtggctgaa cgttacggcg
    31021 tacaggcgct ggtcaccggc gaagcgctgg gacaggtgtc cagccagacg ctaaccaacc
    31081 tgcgcctgat tgataacgtc tccgatacgc tgatcctgcg tccgctgatc tcttacgaca
    31141 aagagcacat catcaacctg gcccgccaga ttggcaccga agattttgcc cgcaccatgc
    31201 cggaatactg cggcgtgatc tccaaaagcc cgactgtgaa agcggttaag tcgaagattg
    31261 aagcggaaga agagaagttc gacttcagca ttctcgataa agtggttgag gaagcgaata
    31321 acgttgatat ccgcgaaatc gcccagcaga ccgagcagga agtggtggaa gtggaaaccg
    31381 tcaacggctt cggcccgaac gacgtgatcc tcgatatccg ttctgttgat gaacaggaag
    31441 ataagccgct gagagtggaa gggattgatg tggtttcttt gccgttctat aaactgagca
    31501 ccaaatttgg cgatctcgac cagaacaaaa cctggctgtt gtggtgtgag cgcggggtga
    31561 tgagccgtct gcaggcgctc tatctgcgcg agcagggctt taacaatgtg aaggtgtatc
    31621 gcccgtaatt tgtggttttt acgtcgcatc tggtcagatg cgacgtttac cgcatccgac
    31681 actactcgta ataattataa atccctgccg ccatcaccag ttgtgatgcc acctcatggg
    31741 ccttctcacg cccaaccagc agatcgataa ttttcagccc aaaatcaata gccgtacctg
    31801 gcccctgact ggtcagcaat tttacccggg catcccagac gacgcgcttg tcctgccatt
    31861 gttcggcggg aattttatct ttcagcgtcg ggaagccggt catattgcca atcgggaaga
    31921 tatcgtgcgg gaccagcacg gtggctggtg cggcgcaaat agccgcgacg atacgcccgg
    31981 aacggtggaa ctgtttaacg gtttcaacca gcagggtgct atcacgaaaa cactctgcgc
    32041 ctttgatgcc accaggcagc acaatcacgt catattcgcc atcagctact tcgaccagcg
    32101 gcgcatccgc cagcagcttt acgccgcgcg agcaggtaat tgccaggtta ccatcgctgg
    32161 cgacgctggc agtggtgact ttgataccgc cgcgaaccag cagatcgata gtggtgacgg
    32221 cttcagtctc ttcactacca ggggcgaggc aaaccagtgc cgatgcgctc atattcactc
    32281 tcctttcttt ttaccatttc aaacaggcgg gtgttttccg gtacggcaat cccatgcgcg
    32341 cgggcgcggc gtaagagaaa accattgata tagtcgattt cggtgtggcg cagcgcgcgg
    32401 atatcctgca acatcgacga gatattttcc gctgtggcat caatcacctg catcacgtaa
    32461 tcacgcaaat cttctgctga agtatgatgc ccttcgcgct cgattactgc cgcgacttcc
    32521 tcgcatatct gcataatttc ttgcggatga tgacgtaatt caccgttcgg gcaattccag
    32581 atggcagtca gtggattaat cacgcagttg acggccagct tgcgccacag ctcggcgcga
    32641 atattgttat gccaggcaac gtcaggcaac acggtttgta aaatatccgc cagataactg
    32701 taatcgccat cctgttgccg tgccggacca atatgcgtga taccgtttgc cacatgaata
    32761 atgacattgc cgtcgcggcg agctgcatgg gtggtggtgc ccatcagtaa tggctgctga
    32821 atgttttgca actcttcgat ggtgcccatg ccgttgtgaa ttaacagtat tggcgtggtt
    32881 acaggcagtg tggacgcgag gcttttgacg gcatcggaaa cctgccatgc tttcagcgtc
    32941 accaggagca gatcgctggt ggcgagaaaa tcgggatcgt tggcggtcag cgattcgtta
    33001 aatatcgaac catctgtctc aaccagattc acactacaat aaggttgcgg tacgcgcagc
    33061 cagccctgaa cgtcgtgacc ctgtttacaa agtgctgtaa gccataattg ccctaaggca
    33121 ccgcatccca atacggtaat tttcattgtt cctcctcacc cgcaaccact ccgggtgttc
    33181 aataaggcta tcccttaatt gtgcatgctg ttgcgactat gcacaattaa gggatacgtc
    33241 ctggtgcagg actgttggtt atttaacttt gcaggtatta tgcttcgcat caaaaatgaa
    33301 gggagaggaa aagatgccat ctttcgatat tgtctctgaa gttgatcttc aggaagcacg
    33361 taacgcggtc gataacgcga gccgcgaagt ggagtcccgt tttgacttcc gtaacgttga
    33421 agcctcattt gagctgaatg acgccagcaa aaccatcaaa gtgttgagcg agtccgactt
    33481 ccaggtcaat cagttgctgg atattctgcg tgccaagttg ttgaagcgcg gcattgaagg
    33541 cagttcactg gatgtaccgg aaaatatcgt tcatagcggt aaaacctggt ttgtggaagc
    33601 gaaactgaaa cagggcattg agagcgcgac tcagaagaaa atcgtcaaaa tgatcaaaga
    33661 cagcaaactg aaagtgcagg cgcaaattca gggcgatgag atccgcgtaa cgggtaaatc
    33721 tcgtgatgat ttacaggctg tcatggcgat ggtacgtggt ggcgatctgg gtcagccgtt
    33781 ccagttcaaa aacttccgcg attaatcgcg acaacctgtg ctttgttcat gtcagatgcg
    33841 gcatgaacgc ctgattcggc ctacaaaggt ttgtaactca ggaacttagt aggcctgata
    33901 agcgtagcgc agcaggcaat ttcgcctcgc ttttatgcct ggcgaattgc ctgttcaacc
    33961 tcaaagcgat tcgtcacttt gctgtcgatt ttcacataag ctgaatgttc ttcttctgca
    34021 atcaacactt ctttgatgcc ttcagtttcc agcaaacgca cttttaacgc ctcgtttgcg
    34081 gcaatgttcg ccgggatttc aatgcgcaag ctgctgacat acggcggttc tttcatggta
    34141 ctggcgactg ccagccacac tgcggccagc attgcgccag cgagaaatac gccctgaccg
    34201 tcaaacatgc cgtcaatcca gccgcccagc gaaccgccaa tcgccacgcc aagaaactgg
    34261 ctggtggagt aaacacccat cgccgtacct ttgtaacctg ctggcgattc tttactgata
    34321 agtgaaggca gaagggcttc catcaaatta aacgccacaa agaaaagctg cacgccgacc
    34381 accagttgcc agaactgcgt ttgtgcgttc cacaacacaa tttccgcaac cacgatcaac
    34441 ccgacgcaga agacaaagac ttgcttcatt ttgcgcttaa cttcagcgta gataatgaaa
    34501 ggcacgaccg agccaaaggc gattaacatc gttgccaggt agaccttcca gtgttcagcc
    34561 gccggtaacc ccgcatcagc catctgtccg ggcagggcaa caaaggtcga catcagcaaa
    34621 atatgcagac acataatgcc aaagttgagt ttcagcagcc gcggttccgc cagcacttta
    34681 ctgaaactgc ctttcaccat tccggactca cgattaagta cgtgagtgct actgttgggc
    34741 acaacccaaa tggtcaacgc aatgccggtt gttgccagaa tagcgatcat ccagaacagc
    34801 gcgtgcagcc caagtttgtg agtgatgatc gggccaagca ccatcgcaat ggcaaaggta
    34861 atgccaaagc tcacgccgat aaacgccatc gctttggtgc ggttttgttc gcgcgtgaga
    34921 tcggaaagca gcgccataac ggcagcagca atcgcaccgg agccttgtag cgcccggccc
    34981 agaataattc cccagatgga gtcagagagc gcagcgataa cgctaccggc ggcaaacacc
    35041 gccagcccac cgacaattaa tggtttgcga ccaatgcggt ctgaaagcag gccaaacgga
    35101 atctgaaaaa cggcctgagt cagaccataa ataccaatgg caataccgat caatgcttcg
    35161 ctggcacctt gcagagccat gccgtacgtg gtcagaaccg gcagaaccat gaacatgccc
    35221 agcatgcgta atgaaaatac ggtccctaaa ccccaggtcg cgcgccgctc acctggcgtc
    35281 attttataat cgttcattac cacctctgtt ttaaattcgc gactagtgta aagcggcaaa
    35341 cgaatggggt aaacatgcgg attttttagt aaatatttcg agagggatat gattctaatg
    35401 ctaagaaaaa aggtgccgta gcaccttttt aactgagagg ttgtgttacc acacagcagc
    35461 cagcagcgta tgcgagtccg gcaccataaa atcaacggac atcatcaccg agagggcagt
    35521 gatggcgatg atagagaaac cgaacagctt gcgcgcccag attctgtcat cagcaacttt
    35581 atagccgcgc agagccatgc ctaaccacca gacgctcacc gcagcggcga ccaccagata
    35641 tttataccca gcgtagccgc caagagagag catcagcgtg gcaacggcaa aggcgatgat
    35701 atacagcgtg atgtgattct tcgccaccga aatgcctttc accaccggca gtaccggaat
    35761 gtttgccgcc tgataatcct taaagcggaa aatggctatg gcataagagt gaggcatctg
    35821 ccacaggctg aagatagcca gcaggatcgc tgcgccgcta tcgaactcgc cggttaccgc
    35881 acagtaaccg atcaccggcg gcgcagcgcc ggagagcgaa ccaatcaatg tgccgtagac
    35941 agaatgacgt ttcatgtaca ggctataaac gccgacataa accacaaagc ccatcacccc
    36001 cagccagcag gccagcggat tcgcgccaaa ccacagcagc ataaagccag caatacccag
    36061 caaggtggcg tacaccagcg agacagcagg agagatcagg cctttaacca gcacccgatt
    36121 cttcgtcctt tccatctttc tgtcgatatc cctgtcgatg tagttgttaa acacacaacc
    36181 cgacgccaca accagtgaca ccccaaccag cgtgtagata aacaggggat aatcaatgct
    36241 gccctttgag gccaacagga atcccccaat caccgagatc aggttgccaa agatgatgcc
    36301 tggtttcgtt acttgcaggt attgcttaaa tatcataacc gccgctctta gtgcatcatc
    36361 atgttgtagt tgaggttcca cataatccaa atggagccca caaccaggat agcgatgatt
    36421 agcacggtga agacaaatgc cgtcatattc cagccttcat ctgatttggt attcatgtgc
    36481 aggaagcaca ccagatgcac cagaatctgt accactgcca ttgccaggat tgttcccaga
    36541 attacggccg gagaggcagc ccctgtcatc accatccaga acggaattac cgtcaggatt
    36601 atcgacagga taaagcctgt catgtaggtc tttacgctgc catgggatgc gccactgctt
    36661 gcgttagaat gactcattac atcgccccca tcagataaac aacagtgaac acacagatcc
    36721 aaaccacatc caggaagtgc cagaacaggc tcaggcacat gatgcgggta cggttagtgc
    36781 tggtcaggcc gcgacgggcg atttgcacca tcagcaccgc catccagata agaccggaag
    36841 tgacgtgcag accgtgcgtg ccgaccagcg caaagaatgc tgacaggaag ccgctgcgat
    36901 ccggacccat gccgttaaca atcaggtgat ggaattcata gatttccatc ccgataaatc
    36961 cggcaccaaa caaccatgtc aacgccagcc aggagatcac ctggcttttg ttgtttttgt
    37021 acatggcgat agccgccatg ccgtaggtga tggagctgaa caacagcaag aaagtttcaa
    37081 ccagaacgaa cggcagttcg aaaatgtcct tacctgtcgg cccacctgcg gtgccgttca
    37141 ccagaacggc ataggtagca aacaagatag agaacagaat gcagtcgctc atcaggtaga
    37201 tccaaaatcc gaagattttg gttccgcctg catcgtggtg cccgtgttcg tgcgcgtggg
    37261 cagtcgcgtg cgtcaaagta tcagttgcca tttttcagcc ctgccttagt aatctcatcg
    37321 aaatgctggt tttccagttt ttcgatttct gccaccggca cgtagtaatc cacgtcctcg
    37381 tcgaagcttt tcacgatcca ggtgatgatc atgcctgcga agccaacaat cgccagccac
    37441 cagatatgcc agatcatggc gaaaccgaag atggtggaga aagccgcaat gacgataccg
    37501 gcaccgctgt ttttcggcat atgaatttct tcatagtggt caggcttttt gtacgcttcg
    37561 cctttctctt tcatttccca gaatgcatca cgttcgtgaa cgtgcggcac aacggcaaag
    37621 ttatagaacg gaggcgggga agaggttgcc cactccagcg tacggccacc ccacgggtcg
    37681 ccagtcaggt cacggttctg gtcgcggtca cgaatagaaa cgtacatctg aataacgagg
    37741 cagagaatac ccagcgcaat cagtaccgca ccgctggctg caatcatcag catggtgtgg
    37801 aactgcgggt caatctgctg gctcaaacga cgggtcatgc ccatgaagcc cagcgcatac
    37861 agtggcataa aggcaacgaa gaagccgatg atccagaacc agaacgcgcg tttaccccag
    37921 gtttcgttca gtttgaaacc gaacgcttta ggccaccagt aggtcatccc tgcgaagcag
    37981 ccgaagacca cgccgccgat gatcacgtta tggaagtgcg caatcaggaa caggctgtta
    38041 tgcagaacga agtccgcgcc cggtactgcc agcagcacgc cagtcatccc gcccaccgag
    38101 aaggtgacga taaaaccgat ggtccacagc atcgcagaat ggaacacgat gcggccctga
    38161 tacatggtga acagccagtt gaagatcttc accccggtcg ggatggcgat aatcatcgtg
    38221 gtgataccaa agaaggcgtt tacgttcgcg cccgcaccca tcgtaaagaa gtggtgcagc
    38281 cagacgatga acgacagcac ggtgatacag acggttgccc ataccagcga ggtataacca
    38341 aacagacgtt tacgcgagaa ggttgccgca atttcggaga acacaccgaa tacaggcagg
    38401 atcaagatgt aaacttccgg gtggccccag gcccaaatca ggttgatata catcatcatg
    38461 ttgccaccca tatcgttggt aaagaaatgg gtgcccagat agcgatccag ggtcaacaac
    38521 gcgacggtaa ccgtcagaat tgggaaggaa gcaataatca gtacgtttgc gcacagtgat
    38581 gcccaggtaa atactggcat cttgaacatg gtcatgcccg gtgcgcgcat ctttagaatg
    38641 gtaacgaaga agttgatacc ggtaagcgtc gtacctatac cggatagctg gagactccat
    38701 atccagtaat cgacaccgac tcccggactg tactctattc ccgatagcgg tggataggcc
    38761 agccagccgg tctgcgcaaa ttcgcccacg ccgagagaaa cgttaaccag aatcacacca
    38821 acaacggtaa accagaagct taagttgttg aggaacggga acgcaacgtc acgcgcgccg
    38881 atctgcagtg gaaccaccag gttcatcaga ccgataacga aaggcatcgc cacgaagaag
    38941 atcataatca cgccgtgcgc ggtgaagatc tgatcgtagt ggtgaggtgg caggaagccc
    39001 gcttcgcccg ccgaggcaag agcctgctgg ctacgcatca taatggcgtc agcaaaacca
    39061 cgcagcaaca tcacaatcgc cacgatgata tacatgatac cgagacgttt atggtcaacg
    39121 gaggtcagcc actctttcca caggtaggtc cacttaccga agtaagtgat caggccaacg
    39181 agcgccagac ctcccaaaat aatgccagcg atcgtaacca tgacgatagg ttcatggaac
    39241 gggactgcat caagtgataa ttttccgaac atctttattc ttcctcaacc cctttaatgg
    39301 gcggattccg cgtggctcat gtccatgcct tccatacctt catgtgcgct gtgctcacct
    39361 tctggctggg tcatgtccat gctcttaccg tgagccataa acttgttaat cacatcggca
    39421 aacaagtctg gtttcacgtt ggagaaatat tccacctggt tgtattcgct aggcgcggcc
    39481 agtttttcga acgctgccat gtcagacatg ctgttcggcg actgtttcgc ttttgcgacc
    39541 cattggtcga atgcggcgcg atccggtgtt gcaatagctt tgaacttcat gcctgagaag
    39601 cccgggccgc tgtagctggc ggagataccg tcataagtac cgggttcgtt ggcgatcaga
    39661 tgcaggcgag tctgcatacc ggccatggca taaatctggc tacccagacg cggaatgaag
    39721 aaggagttca tcacggagtt ggaggtcact ttgaagtaca ccggagtgtt cgccgggaaa
    39781 gcgatttcat tcacggttgc aatgccctgt tccgggtaga tgaagaacca tttccagtcc
    39841 atggaaacca cttcgatggt aatgggcttc tcgtcgtgtg ccagcggctt gctaggctca
    39901 agagcgtgag tggttttcca ggtcagtacc gcaaggaaga tgatgattaa gataggtacc
    39961 gtccagacca cagcttccac tttattggag tgtgaccagt tcgggctgta cttagcatct
    40021 ttattgctcg cacggtactt ccaggcgaaa ccaacagcca tcaagattgc gggaataacg
    40081 acaatcaaca tcaggccaaa tgccgtcagt atcagtgaac gttgctccag accaatctgt
    40141 cctttgggat ctaacagcgc agaattacag ccactgagca atacagtgcc tgcaaataat
    40201 gacaaccatc ccaaactttt attgtatttc ctgagtctca tttaacgacc tcaattccac
    40261 gggatctggt ggcgtttaaa gtgtgtgggc attttacggg aaggttacat tactgtaaac
    40321 atgattaaat tcctgttact tggatttggc tgtcaggtca catatgttgc aaaacatatc
    40381 aagttgattt taagaagctg ttgcaaaagg gcgttataac gaaaggggag tgcagaagga
    40441 gtttgggtgg gcaattggta taatcaatat aaaataaaac aattatttaa caaataatta
    40501 tcattgaggt gacaactggg tggtgagaaa ataaggactc attagctgaa tcgtgtaaga
    40561 aaaatagaat tcataaattt cgtcaataat ttccaaacgc taagccgcac aaaagaacaa
    40621 atattaataa gcgatgctga tgtttattta gccgtaataa ttacggcgag tgattactac
    40681 agctaaataa tattacagat tacgtcagat gtgtttttcg tagcgccaga taatccagca
    40741 aaccaccaag cacaacgcca gaaagggcga ctaaaacccc gacttccagc agcgcaggca
    40801 ggaaagagaa gtgcgtcaaa tccagcgcgt ccattgtcaa cagcagtaac cacacggcca
    40861 acaggctgat acccgccgcc agtgtccaca tggcaaaggc ataacctgca ggatattcgg
    40921 tacgggagat aaagttgcca tttactcgtg tatattcaag cgtctggcgg caaaccagca
    40981 gcaaaataag ccctggtacg gcagcggcga cggagaatag atagaatgtc gaccagccat
    41041 gtgcttcaac aaaccaaccc gccacagggc cgacataaac tcgccctaca gcagaaagcg
    41101 ctgagagcag ggcaaattga gtagcggaaa atgacttatt acacagcgtc attaacagcg
    41161 cgacaaaggc tgatgtaccc atcccgccac agaggttttc gaaaaagacg gctgcgccca
    41221 tgctgtagag atgcttatca gtaatcgaca gcagccagta accggcgtta gatgcacctt
    41281 gtaaaatgcc gaaaatcagc agtgcacgga acagcgacag gcgctgcatc aaaatcccac
    41341 cgtacaatgc gccaacaatg gtcgccagta agccaagcgt tttgttaacc acgcctactt
    41401 cacccgcatc aaacccgacg ccgcgaatca aaaacgtggt tgtcaggctc atagcgaatg
    41461 cgtcgcccag cttatacagc acgataagaa gcaaaataag ccaggcatta ttgcgaccaa
    41521 agaaatctcg cagaggtgca acaaccgctt gttccagcgt tttgggcaca ggaatggtgt
    41581 cggtgggttc tggtgcaagc aacgtcgcga taatacaggg gatcaacagc gccgccatca
    41641 accagtacat gccctgccag cccagccatt tatctgccag ccacagggct aagccgccgg
    41701 aaaccagcat ccctaaacgg taacccagca cgctgattgc cgcgcccgca ccacgttctt
    41761 ctgccgggag cacatcggtt ttccacgcat cgaagacaat atcctgggag gcagagcaaa
    41821 aagcgatcac caccgccagt gccgccatcc agcggagctg ggtgcctggt tcgagaaaac
    41881 ccatcgccgc aatggcgact aataacagaa tttgcgtggc gagcagccaa ccgcgccgcc
    41941 gcccgaaaaa tgggggcgtg tagcggtcca tcagtggtga ccagaggaat ttaaaaacgt
    42001 aagcctggcc taccagagag aagaaaccaa tggttttgag atcgatattc tcgaccgtca
    42061 tccaggcctg taaggtgccg gacgtcaggg cgagcggtag cccggaagca aaacccagga
    42121 tcagcaatat ggctgaacgc ggctgttgaa aaatacgtaa atattgactg gacatgggcg
    42181 tctacaggcc cggcttgcac cgggccagag ggcagaaatt aacgcgcgtt ctgcttgatg
    42241 aattcgtgga tgctggtgtc ctgagacata tcagcgatgg tatcggtcag cacactgtta
    42301 accgcatcgg cgatattttt gttggaggcc tggaacgcac cttcaacgtt gtagctggca
    42361 cgatagtttt tggtcatttt gttgccattc ttcgcggtag cgatgatggc gatatccgct
    42421 ttggtcgcga tgttgtagcg cacgttgccc tgggacacgt cagcatacag ttggctaacg
    42481 atgatttgca gattaaccgg gccattcgga ccaaccatgt atccacgggc ggtcatctgt
    42541 ttttccagca cttcttgcag caggaaacgc agatcgcggg aggcggtcag ggtaacgatt
    42601 tgattatcgc gggtgacttt tgccagcgcc tgatcggtac gctgatcggc accattaatg
    42661 cttacggtga cgcccatcag gcttggatcc tgctgtggca gtgtaatcgt cggggaaact
    42721 tcgatagttg ttggcggttt tgcgcatcct gcaagcataa acagagcaac taacgggaag
    42781 aggatttttt tgaacatgtt cgggctctca gagactctta agcgtgtttg gtaaaaattc
    42841 ccgccatcat aacattgcca acggcgaggg gaagtgggta aggcatgtaa attcatcatg
    42901 ttgacgaaat aatcgcccct ggtaaaagaa acgctgatgc gaggcctgtg tttcaatctt
    42961 taaatcagta aacttcatac gcttgacgga aaaaccagga cgaaacctaa atatttgttg
    43021 ttaagctgca atggaaacgg taaaagcggc tagtatttaa agggatggat gacatctcag
    43081 cgttgtcgga ggagatattt catgatgata cgtgagcgga tagaagaaaa attaagggcg
    43141 gcgttccaac ccgtattcct cgaagtagtg gatgaaagct atcgtcacaa tgtcccagcc
    43201 ggctctgaaa gccattttaa agttgtgctg gtcagcgatc gttttacggg tgaacgtttt
    43261 ctgaatcgtc atcgaatgat ttacagtact ttagcggagg aactctctac taccgttcat
    43321 gcgctggctc tgcatactta cactattaag gagtgggaag ggttgcagga caccgtcttt
    43381 gcctctcctc cctgtcgtgg agcaggaagc atcgcgtaaa aacgcatttg caactgtcgg
    43441 cgcttttcca gtatgttgct aaagatttta tgaaaaacgg cctgcgggcc gttttgtttt
    43501 gtctggattt tgcgcttttt gcccagcatt cagacgaaaa tcgcccggga attgtgaaaa
    43561 aatacgcgac agcgcgcaat aaccgttctc gactcataaa agtgatgccg ctataatgcc
    43621 gcgtcctatt tgaatgcttt cgggatgatt ctggtaacag ggaatgtgat tgattataag
    43681 aacatcccgg ttccgcgaag ccaacaacct gtgcttgcgg ggtaagagtt gaccgagcac
    43741 tgtgattttt tgaggtaaca agatgcaagt ttcagttgaa accactcaag gccttggccg
    43801 ccgtgtaacg attactatcg ctgctgacag catcgagacc gctgttaaaa gcgagctggt
    43861 caacgttgcg aaaaaagtac gtattgacgg cttccgcaaa ggcaaagtgc caatgaatat
    43921 cgttgctcag cgttatggcg cgtctgtacg ccaggacgtc ctgggcgacc tgatgagccg
    43981 taacttcatt gacgccatca ttaaagaaaa aatcaatccg gctggcgcac cgacctatgt
    44041 tccgggcgaa tacaagttgg gtgaagactt cacctactct gtagagtttg aagtttatcc
    44101 ggaagttgaa ctgcagggtc tggaagcgat cgaagttgaa aaaccgatcg ttgaagtgac
    44161 cgacgctgac gttgacggca tgctggatac tctgcgtaaa cagcaggcaa cctggaaaga
    44221 aaaagacggc gctgttgaag cagaagaccg tgtgaccatc gacttcaccg gttctgtaga
    44281 cggcgaagag ttcgaaggcg gtaaagcgtc tgatttcgta ctggcgatgg gccagggtcg
    44341 catgatcccg ggctttgaag acggtatcaa aggccacaaa gctggcgaag agttcaccat
    44401 cgacgtgacc ttcccggaag agtaccacgc agaaaacctg aaaggtaaag cagcgaaatt
    44461 cgctatcaac ctgaagaaag ttgaagagcg tgaactgccg gaactgactg cagaattcat
    44521 caaacgtttc ggcgttgaag atggttccgt agaaggtctg cgcgctgaag tgcgtaaaaa
    44581 catggagcgc gagctgaaga gcgccatccg taaccgcgtt aagtctcagg cgatcgaagg
    44641 tttggtaaaa gctaacgaca tcgacgtacc ggctgcgctg atcgacagcg aaatcgacgt
    44701 tctgcgtcgc caggctgcac agcgtttcgg tggcaacgaa aaacaagctc tggaactgcc
    44761 gcgcgaactg ttcgaagaac aggctaaacg ccgcgtagtt gttggcctgc tgctgggcga
    44821 agttatccgc accaacgagc tgaaagctga cgaagagcgc gtgaaaggcc tgatcgaaga
    44881 gatggcttct gcgtacgaag atccgaaaga agttatcgag ttctacagca aaaacaaaga
    44941 actgatggac aacatgcgca atgttgctct ggaagaacag gctgttgaag ctgtactggc
    45001 gaaagcgaaa gtgactgaaa aagaaaccac tttcaacgag ctgatgaacc agcaggcgta
    45061 atttacgtag tataacgctc taaattcgca caaaggcccg tcaccgccag gtggtgggct
    45121 tttttttgtc atgaattttg catggaaccg tgcgaaaagc ctctttcggt gttagcgtaa
    45181 caacaaaaga ttgttatgct tgaaatatgg tgatgccgta cccataacac agggactagc
    45241 tgataatccg tccataaggt tacaatcggt acagcaggtt ttttcaattt tatccaggag
    45301 acggaaatgt catacagcgg cgaacgagat aactttgcat cccatatggc gctggtgccg
    45361 atggtcattg aacagacctc acgaggtgag cgctcttttg atatctattc tcgtctactt
    45421 aaggaacgcg tcatttttct gactggccag gttgaagatc acatggctaa cctgattgtg
    45481 gcgcagatgc tgttcctgga agcagaaaac ccagaaaaag atatctatct gtacattaac
    45541 tctccaggcg gggtgattac tgccgggatg tctatctatg acaccatgca gtttatcaag
    45601 cctgatgtca gcaccatctg tatgggccag gcggcctcga tgggtgcttt cttgctgact
    45661 gcaggggcaa aaggtaaacg tttctgcctg ccgaattcgc gcgtgatgat tcaccagccg
    45721 ttgggcggct accagggcca ggcgaccgat atcgaaattc atgcccgtga aattctgaaa
    45781 gttaaagggc gcatgaatga acttatggcg cttcatacgg gtcaatcatt agaacagatt
    45841 gaacgtgata ccgagcgcga tcgcttcctt tctgcccctg aagcggtgga atacggtctg
    45901 gtcgattcga ttctgaccca tcgtaattga tgccagaggc gcaactgtgc cgctatactt
    45961 atccagggcg gcacaacgct gtaagcggct tgcgcctgag aatggcattt gcgtcgtcgt
    46021 gtgcggcaca aagaacaaag aagaggtttt gacccatgac agataaacgc aaagatggct
    46081 caggcaaatt gctgtattgc tctttttgcg gcaaaagcca gcatgaagtg cgcaagctga
    46141 ttgccggtcc atccgtgtat atctgcgacg aatgtgttga tttatgtaac gacatcattc
    46201 gcgaagagat taaagaagtt gcaccgcatc gtgaacgcag tgcgctaccg acgccgcatg
    46261 aaattcgcaa ccacctggac gattacgtta tcggtcagga acaggcgaaa aaagtgctgg
    46321 cggtcgcggt atacaaccac tacaaacgtc tgcgcaacgg agataccagc aatggcgtcg
    46381 agttgggcaa aagtaacatt ctgctgatcg gtccgaccgg ttccggtaaa acgctgctgg
    46441 ccgaaacgct ggcgcgcctg ctggacgttc cgttcaccat ggctgacgca accacgctga
    46501 ccgaagccgg ttatgtgggc gaagacgttg aaaacatcat tcagaagctg ttgcagaagt
    46561 gcgattacga cgtacagaaa gcgcagcgcg ggattgtcta catcgatgaa attgacaaga
    46621 tttctcgtaa gtcagacaac ccgtccatta cccgagacgt ttccggtgaa ggcgtacagc
    46681 aggcactgtt gaaactgatc gaaggtacgg tagctgctgt tccgccgcaa ggtgggcgta
    46741 aacatccgca acaggaattc ttgcaggttg atacctctaa gatcctgttt atctgtggcg
    46801 gtgcgtttgc cggtctggat aaagtgattt cccatcgtgt agaaaccggt tccggcattg
    46861 gttttggcgc gacggtaaaa gcgaagtccg acaaagcaag cgaaggcgag ctgctggcgc
    46921 aggttgaacc ggaagatctg atcaagtttg gtctgatccc tgagttcatt ggtcgtctgc
    46981 cggttgtcgc aacgttgaat gaactgagcg aagaagctct gattcagatc ctcaaagagc
    47041 cgaaaaacgc cctgaccaag cagtatcagg cgctgtttaa tctggaaggc gtggatctgg
    47101 aattccgtga cgaggcgctg gatgctatcg ctaagaaagc gatggcgcgt aaaaccggtg
    47161 cccgtggtct gcgttccatc gtagaagccg cactgctcga taccatgtac gatctgccgt
    47221 ccatggaaga tgtcgaaaaa gtggttatcg acgagtcggt aattgatggt caaagcaaac
    47281 cgttgctgat ttatggtaag ccggaagcgc aacaggcatc tggtgaataa ttaaccattc
    47341 ccatacaatt agttaaccaa aaagggggga ttttatctcc cctttaattt ttcctctatt
    47401 ctcggcgttg aatgtggggg aaacatcccc atatactgac gtacatgtta atagatggcg
    47461 tgaagcacag tcgtgtcatc tgattacctg gcggaaatta aactaagaga gagctctatg
    47521 aatcctgagc gttctgaacg cattgaaatc cccgtattgc cgctgcgcga tgtggtggtt
    47581 tatccgcaca tggtcatccc cttatttgtc gggcgggaaa aatccatccg ttgtctggaa
    47641 gcggcgatgg accatgataa aaaaattatg ctggtcgcgc agaaagaagc ttcaacggat
    47701 gagccgggtg taaacgatct tttcactgtc gggaccgtgg cctctatatt gcagatgctg
    47761 aaactgcctg acggcaccgt caaagtgctg gtcgaggggt tacagcgcgc gcgtatttct
    47821 gcgctctctg acaatggcga acacttttct gcgaaggcgg agtatctgga gtcgccgacc
    47881 attgatgagc gggaacagga agtgctggtg cgtactgcaa tcagccagtt cgaaggctac
    47941 atcaagctaa acaaaaaaat cccaccagaa gtgctgacgt cgctgaatag catcgacgat
    48001 ccggcgcgtc tggcggatac cattgctgca catatgccgc tgaaactggc tgacaaacag
    48061 tccgttctgg agatgtccga cgttaacgaa cgtctggaat atctgatggc aatgatggaa
    48121 tcggaaatcg atctgctgca ggttgagaaa cgcattcgca accgcgttaa aaagcagatg
    48181 gagaaatccc agcgtgagta ctatctgaac gagcaaatga aagctattca gaaagaactc
    48241 ggtgaaatgg acgacgcgcc ggacgaaaac gaagccctga agcgcaaaat cgacgcggcg
    48301 aagatgccga aagaggcaaa agagaaagcg gaagcagagc tgcagaagct gaaaatgatg
    48361 tctccgatgt cggcagaagc gaccgtagtg cgtggatata tcgactggat ggtacaggtg
    48421 ccgtggaatg cgcgtagcaa ggtcaaaaaa gacctgcgtc aggcgcagga aatccttgat
    48481 accgaccatt atggtctgga gcgcgtgaaa gatcgtatcc ttgagtatct tgcggttcaa
    48541 agccgtgtca acaaaatcaa gggaccgatc ctctgcctgg tagggccgcc gggggtaggt
    48601 aaaacctccc tgggtcagtc catcgccaaa gccaccgggc gtaaatatgt ccgtatggcg
    48661 ctgggcggcg tgcgtgatga agcggaaatc cgtggtcacc gccgtactta catcggttct
    48721 atgccgggta aactgatcca gaaaatggcg aaagtgggcg tgaaaaaccc gctgttcctg
    48781 ctcgatgaga tcgacaaaat gtcttctgac atgcgtggcg atccggcctc cgcactgctt
    48841 gaagtgctgg atccagagca gaacgtagcg ttcagcgacc actacctgga agtggattac
    48901 gatctcagcg acgtaatgtt tgtcgctacg tcgaactcca tgaacattcc ggcaccgctg
    48961 ctggatcgta tggaagtgat tcgcctctcc ggttataccg aagatgaaaa actgaacatc
    49021 gccaaacgtc acctgctgcc gaagcagatt gaacgtaatg cactgaaaaa aggtgagctg
    49081 accgtcgacg atagcgccat tatcggcatt attcgttact acacccgtga ggcgggcgtg
    49141 cgtggtctgg agcgtgaaat ctccaaactg tgtcgcaaag cggttaagca gttactgctc
    49201 gataagtcat taaaacatat cgaaattaac ggagataacc tgcatgacta cctcggtgtt
    49261 cagcgtttcg actatgggcg cgcggataac gaaaaccgtg tcggccaggt aaccggtctg
    49321 gcgtggacgg aagtgggcgg tgacttgctg accattgaaa ccgcgtgcgt tccggggaaa
    49381 ggtaaactga cctataccgg ttcgctcggc gaagtgatgc aggagtctat tcaggcggcg
    49441 ttaacggtgg ttcgtgcgcg tgcggaaaaa ctggggatca accctgattt ttacgaaaaa
    49501 cgtgacatcc acgtccacgt accggaaggt gcgacgccga aagatggtcc gagtgccggt
    49561 attgccatgt gcaccgcgct ggtttcttgc ctgaccggta acccggttcg tgccgatgtg
    49621 gcaatgaccg gtgagatcac tctgcgtggt caggtactgc cgatcggtgg tttgaaagaa
    49681 aaactactgg cagcgcatcg cggcgggatt aaaacagtgt taattccgtt cgaaaataaa
    49741 cgcgatctgg aagagattcc tgacaacgta attgccgatc tggacattca tcctgtgaag
    49801 cgcattgagg aagttctgac tctggcgcta caaaatgaac cgtctggcat gcaggttgtg
    49861 actgcaaaat agtgacctcg cgcaaaatgc actaataaaa acagggctgg caggctaatt
    49921 cgggcttgcc agcctttttt tgtctcgcta agttagatgg cggatcaggc ttgcccttat
    49981 taaggggtgt tgtaagggga tggctggcct gatataactg ctgcgcgttc gtaccttgaa
    50041 ggattcaagt gcgatataaa ttataaagag gaagagaaga gtgaataaat ctcaattgat
    50101 cgacaagatt gctgcagggg ctgatatctc taaagctgcg gctggccgtg cgttagatgc
    50161 tattattgct tccgtaactg aatctctgaa agaaggggat gatgtagcac tggtaggttt
    50221 tggtactttt gccgtcaaag agcgtgctgc ccgtactggc cgcaacccgc agaccggtaa
    50281 agagatcacc atcgctgctg ctaaagtacc gagcttccgt gcaggtaaag cactgaaaga
    50341 cgcggtaaac taagcgtagt ccccagtggg gatgtgacga agttcaaggg cgcatctact
    50401 gatgtgcctt ttttatttgt attcggtgac tttctgcgtc ttgtgggctg acaattgccc
    50461 ccgtttcttg tcacaatagg cctttgcgcg catcgatacg ttgcgtgagg tacacagtca
    50521 tctacagcgg agtgttgtta caccatgatg gacagcttac gcacggctgc aaacagtctc
    50581 gtgctcaaga ttattttcgg tatcattatc gtgtcgttca tattgaccgg cgtgagtggt
    50641 tacctgattg gcggaggcaa taactacgcc gcaaaagtga atgaccagga aatcagccgt
    50701 gggcaattcg agaacgcctt caacagcgag cgtaatcgca tgcagcaaca gctgggcgat
    50761 caatactccg agctggcagc gaacgaaggc tatatgaaaa ccctgcgtca acaggtgctg
    50821 aatcgtctga tcgacgaggc gcttctggat cagtacgctc gtgagctgaa actgggtatc
    50881 agcgatgagc aggttaaaca ggcgattttc gcgaccccag ccttccaggt tgacggcaaa
    50941 tttgataaca gccgctataa cggtatcctc aaccagatgg ggatgaccgc cgatcagtac
    51001 gctcaggcgc tgcgtaacca gctcactacc caacagctga ttaacggcgt tgccggtacc
    51061 gattttatgc tgaaaggtga aaccgacgag ctggcggcac tggtagctca acaacgcgtg
    51121 gtgcgtgaag cgactatcga tgttaacgcg ctggcggcga agcagcctgt gaccgaacag
    51181 gaaattgcca gctactacga acaaaacaaa aacaatttca tgacgccgga acaattccgc
    51241 gtgagttaca tcaagctgga tgccgcgacg atgcagcaac cggttagcga tgcggatatc
    51301 cagagctact acgatcagca tcaggatcaa ttcacccagc cgcagcgtac ccgttacagc
    51361 atcatccaga ccaaaactga agatgaagcg aaagcggtac ttgatgagct gaataagggc
    51421 ggtgattttg ctgcattagc caaagaaaaa tctgccgata ttatctctgc ccgcaacggc
    51481 ggtgatatgg ggtggttaga agatgccact atcccggacg aactgaaaaa tgcgggtctg
    51541 aaagaaaaag gccaactgtc tggtgttatc aaatcttcgg tcggtttcct gattgtgcgt
    51601 ctggacgaca ttcagccagc gaaagtgaaa tcgttagacg aagtacgtga cgacattgcg
    51661 gcgaaagtga aacacgaaaa agccctcgat gcgtactacg cgctgcagca gaaagtgagc
    51721 gatgcggcaa gcaacgacac cgagtctctg gccggtgcag agcaagctgc cggcgttaaa
    51781 gccactcaga cgggttggtt cagcaaagat aatctgccgg aagagttgaa cttcaagcca
    51841 gttgccgacg ctatcttcaa cggcggtctg gtaggtgaaa acggcgcgcc gggcatcaac
    51901 tctgacatca tcaccgtaga cggcgaccgc gcattcgtgc tgcgcatcag cgagcacaaa
    51961 ccggaagcgg tgaaaccgtt ggcagatgtt caggaacaag ttaaggcact ggttcagcac
    52021 aacaaagctg aacaacaggc gaaagtggat gctgagaaac tgctggttga tttgaaagcc
    52081 ggcaaaggtg cggaagctat gcaggctgcc ggtctgaaat ttggcgagcc gaaaacctta
    52141 agccgttccg gtcgtgaccc gattagccag gctgcgtttg cactgccact gccagcgaaa
    52201 gacaaaccga gctacggtat ggcgaccgat atgcaaggta atgtagttct gctggcgctg
    52261 gatgaagtga agcaaggttc aatgccggaa gatcagaaaa aagcgatggt gcaaggtatc
    52321 actcagaaca acgcacaaat cgtctttgaa gctctgatga gtaacctgcg taaagaggcg
    52381 aaaatcaaaa ttggcgatgc gctggaacag caataatcct gaagccgcct cgcaaaaaaa
    52441 tgctttgcag ctgtaacaag aaaaggtcgc tttcgcggcc ttttccattt ctgaacattg
    52501 ccatttgttt actgttttca ctgccgttaa ggtgattcca ctgttaacaa acaaggagaa
    52561 aacagtatga aacacggaat taaagcactg ctcattaccc tgtccctggc ctgtgccgga
    52621 atgtctcata gcgcgctggc ggcagcttct gtggcgaaac cgacaacagt agaaaccaaa
    52681 gcggaagctc ctgcagcaca aagtaaagca gcagtaccgg cgaaagccag tgacgaagaa
    52741 ggcacccggg tcagcattaa taatgccagc gcggaagagt tagcccgcgc gatgaatggc
    52801 gttggcctga agaaagcgca ggcgattgtc agttatcgcg aagagtacgg tccgtttaaa
    52861 acggtggagg atctaaaaca ggtgccgggg atgggcaatt cgctggtgga acgtaatctg
    52921 gcggtattaa ccctgtaatt aatttgcata gtggcaattt tttgccagac tgaagaggtc
    52981 ataccagtta tgacctctgt acttataaca gttcttataa caacaacgta aggttattgc
    53041 gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg acgtttacca gcacgtaaac
    53101 aacgcccgct accttgaatt tctcgaagaa gcccgctggc atgggttgga aaatagcgac
    53161 agttttcact ggatgacggc ccataacatc gccttcgtcg tggtcaatat caatattaac
    53221 tatcgccgcc cggcggtatt aagcgacctg ttaaccatta ccagtcagtt gcagcaatta
    53281 aacggtaaaa gtggcatctt aagccaggtc attacactgg agccggaagg acaggtggta
    53341 gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc attagctctg
    53401 gaaggggaat tgcgcgaaaa gctggagcag atggttaagt aaacgttttg tggtgccgga
    53461 tgctcaagcc gcatccggcg acacccggaa taattacttc aacccggttt tctgcttcat
    53521 cgctgccatc accgtcggtt tatcggccag ataatgattc aaaccgttgg cacgtaaatt
    53581 acacgccgca caatgaccgc aaccgtcgcc tttaatgccg ttataacagg tcagcgtttc
    53641 gttacggact aaatccagct tgccgtaata atctgccagc gcccaggttt ccgctttatc
    53701 aatccacatc agcggcgttt caaaacgaat atctttcgcc atgcccaaac tgacggcatg
    53761 gtttagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat ccgtttcgca
    53821 gacgccggta attacggctt ctgcttttac ctgatacgca tatattgccg ccagcgtcag
    53881 gaacaaaata ttacgccctg ggacaaacgt attcgggata ccatcggctt caggttcata
    53941 atcaggcacc ggaatgctgt cacgcgtcag gctgctgacc gccagctcgt tgagcagcgt
    54001 tacatcaagc accttatgcg cgcgtgcccc cagtttcagc gccagttcgc gtgccacgtc
    54061 gatttctgcg cgatggcgtt gaccgtaatc gaacgtcacg caatggactt catcatattg
    54121 ttgtaatgcc tgcaccagac aggtggtgga atcctggcct ccactgaaca caacgacagc
    54181 acgtttcata gataatcctg cttgaacaat aaaagcgtta tggtaacgcc tgcgattaac
    54241 ccggaccagc tattcattgc gacggcgcgg gaagccaggc ttcggtaaat tcaaaccagc
    54301 cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc gctaatgcga tagtgatagt
    54361 tgaacagcgg tgtcagcgtc gcatctgcca ttaactggct aaaaaccgct ttcagggcat
    54421 taaatcggtt ttcctcatca ggcattactt gcaccgcgtc cagtgtcgat tgcaaatgtg
    54481 catatgctgg agcgtcgaaa acatgtggcc acagtggatc gcaacgcagc cattgctcca
    54541 gggtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca
    54601 gtagggtcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt
    54661 ccgctgccag tgtcgcctgt aggcgttctg ccatggtatg aagttctatc ggtaggtgat
    54721 aaaccagcgt caaggttttc ggtagtttaa cttcatccgg tacttgccaa tgcggaatag
    54781 tccagcctgg cagtaatgca tgactggcag tgatcaggtt ttctccgact tctaacgttt
    54841 gtaataaacc ggattgatga ataatggaga tcacttttcg cgcctgccag agggacagtc
    54901 ggagactttt gcgcaatgtt aaatagcaaa aacctaaact gatgccgcta ctgacctggc
    54961 tgacccgttg cagctcctcc ggtttgccaa tggtgatttg cacgggatgc cgacaactgg
    55021 ttcccatatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct ttaagcagcg
    55081 gatgacgtaa atggtaataa tcatggctct ccaggcgcac cagttccgct gtgaattgtg
    55141 ttaagcgaaa aggacctgtg ccgatcagtg ggaattgcgg atgcgccaga tggctgcaat
    55201 agctcgccag tcggtgcgca agccagtaat cggggcgatg taaaaagaag gtcagacact
    55261 gcggatgggt gacttcaata cgcttcacgc taataaataa ttgatccagt gctggcagtt
    55321 gtaacagcat caataatcgc tggtgtaagt gtgaggcttt tactgcatcg ccgttatgcc
    55381 agtgtagcgt tgaacgaaga taaaagtccc agcgtaaccc gtcagtagag gtttcccagt
    55441 gatgtgctaa atcgccaatc gggcgctgag tattattatc gaagcgggtc aggccggaaa
    55501 atatctgccc ggccagatgc tgctcggcac ggccgggcaa aaagcccggt tgtagcggtt
    55561 cgagcgggcg atagtaggga atacgcaacg tgggcgtatc gttttgccat tgtccgccca
    55621 taaacggctg taacagagtg cgcagctcac ctggggccag ttgcgccagc tccagcacat
    55681 cttgcggctt tccggtttcc agcgcctgtt ccatcatcgc attgcgtagc gattctggcg
    55741 tgaccagaaa gcgtaattgt ccgcgctttc cgcgtcctga ctgcgcctgc cactccagcc
    55801 atcccgcctc ctgtgcctga cgcaacagcg tacgaacatg gcgttcgctg caaaaacagc
    55861 gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggcggaaggt tgccacagac
    55921 gttgatactg gttaagacgg ttgagcagtc gcatataaac ccggaacaat attatttaac
    55981 tattcactat tacttccgta tatatcaggt gatactcaat caccattaac cgtgtcacag
    56041 agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt tattgatgcc
    56101 tgaccatcat ttaggtgaga aaaccctctc tactttggcg cgactgcgtg aacgcgacat
    56161 taccctcact tttgccacgg ggcgtcatgc gctggagatg cagcatattc tcggggcgct
    56221 atcgctggat gcatatttga ttactggcaa cggaacgcgc gtgcattctc tggaaggtga
    56281 acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt atcagcaatg
    56341 ggatacccga gcaagcatgc atatcttcaa tgacgatggt tggtttaccg ggaaagagat
    56401 ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt tatcagataa tcgatgtcaa
    56461 aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg acgatcttac
    56521 acgcttgcag atccagctat acgaagcatt aggcgagcgt gcacatttat gtttttccgc
    56581 cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa ggcgctgcat tgacggtgct
    56641 gacccaacat ttaggtttat cgttacgcga ttgcatggcc tttggtgatg cgatgaacga
    56701 tcgcgaaatg ttaggcagcg tcggtagcgg atttattatg ggcaatgcga tgccgcaact
    56761 gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaatcagg ctgtctctca
    56821 ctatttgacg cactggctgg actatccaca tctaccttat tcccccgaat aacgagatcc
    56881 cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt ttgtgccgtg
    56941 atgcttaaac cgcacagaat aaattgtcgc gatttcacct ttaaaataga attaaaagag
    57001 aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct gctggccttg
    57061 ctacagcagg attgcaccct ctctttgcag gcactggctg aagccgttaa tctgacaacc
    57121 accccctgct ggaagcgtct gaaacggctg gaggacgacg gtatccttat tggcaaagtc
    57181 gccctgctgg atccggaaaa aataggcctc ggcctgaccg cctttgtgct gataaaaacg
    57241 caacatcaca gcagcgaatg gtattgccgc tttgtcacgg tggttaccga aatgccagaa
    57301 gtgctggggt tctggcgcat ggctggtgaa tacgattatc tgatgcgcgt ccaggttgcc
    57361 gacatgaaac gctacgacga gttttataag cgtctggtaa acagtgtacc ggggctgtcg
    57421 gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt acccatcgaa
    57481 taaatatcca gaatcaagtc aggacacaac gcgtgcgatt atttgctcaa ttaagctggt
    57541 atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc attatcgcga
    57601 tgctgcaact ggttccgcca aaagtggttg gtattgttgt tgatggcgtg acagaacaac
    57661 actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt gccgttgtgg
    57721 tttatctcct gcgttacgtc tggcgcgtat tgctgtttgg tgcgtcttat caactggctg
    57781 ttgaactgcg tgaagattat taccgccagc taagccggca gcatcctgag ttttacctgc
    57841 gtcatcgcac gggtgacctc atggctcgtg cgaccaatga cgtcgatcgc gtcgtgtttg
    57901 ccgccggaga aggggtgctg acgctggtgg attcactggt aatgggctgc gctgtgttga
    57961 ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgttg ccgatgccag
    58021 tgatggcgat catgatcaag cgcaacggcg atgccttgca tgaacgcttt aagctggcac
    58081 aggcggcgtt ttccagtctt aatgaccgca cccaggaaag cctcaccagt attcgcatga
    58141 tcaaagcctt tggtctggaa gatcgtcagt cggcgttatt tgccgcggat gccgaagata
    58201 ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgacccg accatctata
    58261 tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg tagttggatg gtggtacagg
    58321 gcagtttaac gctgggccag ctcaccagtt ttataatgta tttaggtctg atgatttggc
    58381 caatgctggc gctggcatgg atgtttaaca ttgtggaacg tggcagcgcg gcgtatagcc
    58441 gaattcgtac catgctggcc gaagcgccag tcgttattga tggtagtgat aaggtgccag
    58501 aaggtcgtgg tgaactggat gtgaatattc gccagtttac gtatccgcag actgaccatc
    58561 ctgcgctgga aaacgtcaat ttcgccctga aacccggtca gatggtaggt atttgcgggc
    58621 cgactggttc cggcaaaagt accctgttgt cgctcattca gcgtcatttc gacgtcagcg
    58681 agggtgatat ccgttttcat gatattcctc tgacgaagtt acaactcgat agctggcgta
    58741 gccgcctggc ggtagttagc cagacaccgt tccttttttc cgacaccgta gcgaacaaca
    58801 tcgcgttggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg cggttagcca
    58861 gcgtgcatga cgatattttg cgtttaccgc aaggttacga tacagaagtg ggcgagcgcg
    58921 gcgtgatgct ttccggtggg caaaaacagc gtatctccat tgctcgtgcg ttattagtca
    58981 acgcggaaat cctcatcctt gatgatgcgc tttcagcggt ggacggacgc acagagcacc
    59041 agatcctgca taacctgcgt cagtgggggc agggaagaac ggtaatcatc agtgcccatc
    59101 gcctttctgc actgacggaa gccagtgaaa ttattgtgat gcagcacgga catatcgccc
    59161 agcgtggcaa tcatgatgag ctggcgcaac aaagtggctg gtatcgcgat atgtatcgct
    59221 atcaacaact ggaggcggcg ctcgacgacg ctccggaaat tcgcgaggag gccatcgatg
    59281 cgtagtttta gccaactgtg gccgactctc aagcgcctgt tagcgtacgg ttcgccgtgg
    59341 cgtaaaccgt tggggattgc ggtcctgatg atgtgggttg cggcggcggc agaagtcagt
    59401 gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa cctgccgttg
    59461 aaagtggttg cagggctggc tgcggcgtat gttgggctgc aactgtttgc cgccgggcta
    59521 cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca acagttgcgt
    59581 accgacgtga tggatgctgc gttacgccag ccattaagcg agtttgatac ccaacccgtc
    59641 gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgacct ctacgttacc
    59701 gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg cgatgctggt ggcaatgttc
    59761 agcctcgact ggcgaatggc gctggtggcg ataatgattt tcccggtggt gctggtggta
    59821 atggtgatat atcagcgtta cagcacgccg attgtccgtc gtgtgcgcgc ttatctggcg
    59881 gatatcaacg acggctttaa cgaaatcatt aatggcatga gcgttatcca gcagttccgt
    59941 cagcaggcgc gatttggcga acgtatgggt gaggccagtc gttcacacta tatggcgagg
    60001 atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct gttttcatcg
    60061 ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ccagcggcac cattgaagtg
    60121 ggcgtgttgt atgcgttcat cagttatttg gggcgactta acgaaccctt aatcgaactg
    60181 accacgcagc aggcgatgct gcaacaggct gttgttgctg gtgagcgcgt ttttgagctg
    60241 atggacggac cgcgccagca atatggcaat gatgatcgcc cgttgcagag cggcaccatc
    60301 gacgtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa gaacattaat
    60361 ctctctgtgc cttcgcgcaa ctttgtggcg ctggtcgggc ataccggcag tggcaaaagc
    60421 accctcgcca gtttattgat gggctattac ccgctaacgg aaggtgagat tcgccttgat
    60481 ggtcgtccat taagtacgct aagtcacagc gcgctgcgcc agggcgtggc aatggtgcag
    60541 caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg acgtgatatc
    60601 tctgaagaac gcgtctggca ggcgctggaa accgtgcagc tggcggagct ggcgcgtagc
    60661 atgagtgatg gtatttacac gccgcttggc gagcagggga ataatctctc agtcgggcaa
    60721 aagcaactgc tggcactggc gcgcgtgctg gtggagacgc cgcaaatcct gatccttgat
    60781 gaggcaaccg ccagcattga ctccgggact gaacaggcga ttcaacatgc tctggcggcg
    60841 gtgcgtgaac atactacgct tgtggtgatt gctcaccgct tatcgactat tgttgatgcc
    60901 gacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca ccagcaactg
    60961 ctggcggccc agggacgata ctggcagatg tatcaactgc aacttgcggg cgaagagctg
    61021 gcagccagcg tgcgtgaaga ggaatcattg agcgcctgaa tagcgcaata tttcatcgtt
    61081 ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt tttcattttc aatcttctta
    61141 acttcctgct ctctttctcg tttttcattt ctggcacacc gcttgcaata ccttcttcgt
    61201 gtagcagaac cattaccgaa ttctgaccgg aggggatcta tgaagctggt gaccgtgata
    61261 atcaaaccat tcaagctgga agacgttcgt gaagcgttat cttccattgg tattcagggc
    61321 ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag ggcatgccga gctgtaccgg
    61381 ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa ttgatgtggc gattgctgat
    61441 gaccaactcg atgaagtgat cgatatcgtc agcaaagcgg cttacaccgg aaaaattggc
    61501 gacggcaaaa tcttcgtcgc cgaattgcaa cgcgtcattc gtattcgtac cggcgaagcc
    61561 gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg aagatagcaa
    61621 cgataaaaac tgggcttact tcactggcga tgctgccggg actggtaatg gctgcacctg
    61681 cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg gtgctgttta
    61741 tgaccgttcc ggggattgcc ctgttttatg gagggctgat tcgtggcaaa aacgtgctgt
    61801 cgatgctgac gcaggtgacc gtgacgtttg cactggtctg tattctctgg gtggtttacg
    61861 gctactcgct ggcgtttggc gaaggcaaca acttcttcgg caacattaac gggttgatgc
    61921 tgaaaaacat tgaactgacg gcggtgatgg gcagcattta tcagtatatc cacgtggcgt
    61981 ttcagggatc gtttgcctgc attaccgtag gcttgatagt tggggcgctg gcggaacgaa
    62041 tccgcttctc agctgtgttg atttttgtgg tggtatggct gacgctctct tacattccga
    62101 ttgcgcatat ggtctggggc ggcggtttgc tggcttctca cggtgcgctg gatttcgcgg
    62161 gtggcacggt agtgcacatt aacgccgcaa tcgccggtct ggtgggcgcg tatctgatag
    62221 gcaaacgcgt gggcttcggc aaagaggcgt ttaaaccgca caacctgccg atggtcttca
    62281 ccgggactgc cattctctat atcggttggt ttggctttaa cgccgggtca gcgggcacgg
    62341 cgaatgaaat cgcggcactg gcatttgtga atactgtggt cgcaaccgcg gcggcaattc
    62401 ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttcactgctg ggagcgtgtt
    62461 ctggcgcgat tgccggtctg gtcggcgtga cgccagcctg tggttacatt ggggttggcg
    62521 gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt accatgctca
    62581 aacgcttgct gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc gtttgtggta
    62641 ttgtcggctg tatcatgacc gggatttttg ccgccagctc gctgggcggt gtgggcttcg
    62701 ctgaaggtgt gacgatgggc catcagttgc tggtacagct ggaaagcatc gccattacga
    62761 ttgtctggtc tggtgtggtg gcatttatcg gctacaaact ggcggatctg acggttggtc
    62821 tgcgtgtacc ggaagagcag gagcgagaag ggctggacgt caacagccac ggcgagaatg
    62881 cctataacgc gtaacaagca ctgcaaaaaa cagccggacg gttttcacct ccggctattt
    62941 ttttaattgt gattacgcat caccccttcc tgaacggtcg aggcaaccag cacgccgtcc
    63001 tgggtataaa actcaccgcg cacaaagccg cgtgcgctgg acgccgaggt gctttccacg
    63061 ctatacagca gccattcatt caaattaaac gggcgatgga accacatgga atggtcaatg
    63121 gtggcaatct gaatccccgg ttcgagaaaa ccgatgccgt gcggctgtag agctaccggc
    63181 aggaaattaa gatcagaagc ataaccgagc agatactgat gaacgcgcag atcatccggc
    63241 acgctaccat ttgcgcggat ccacacctga cgatgtggtt ctgcgacgtg acctttcagt
    63301 gggttatgaa actccaccgg acggacttcc agcggacgat cgcagatgaa tttatctttc
    63361 agcactggcg gcagcaggtg cgccagcgat tgggcgattt gcgtttctga aggtaggcca
    63421 tcaggcgctg gtgcggacgg cattgttttt tgatgctcga aacccgcttc tggtgcctgg
    63481 aaagaggcag tcatataaaa aatcggtttg ccgttttgaa tagcagcaac ccggcgggcg
    63541 ctgaagctgt taccgtcacg cagcgtttcg acatcataaa taatcggctt cttactatcg
    63601 ccagggcgaa gaaagtagct gtgaaaagaa tgtaccagcc gctcgtcagg gacggtctct
    63661 tttgcagcat acaaggcctg acccacgacc tggccgccaa atacctggcg taaacctaaa
    63721 tcttcactct ggccgcgaaa gagtccttcc tcaatttttt ccagatttaa caatgtcagt
    63781 aaatttttta gcgcctgact catataactc tccagtaaca aagctgccgc agcaagccaa
    63841 agtgagttga gtataacgca aatttgccac tggtccgatg ggtgcaatgg tctgaattac
    63901 gggctaatta caggcagaaa tgcgtgatgt gtgccacact tgttgacgtt actattttgt
    63961 taaccactct tccggcgagg aaagttagcc cgctggtgca ttgataataa ggagaagtga
    64021 atgaaactcg tgcacatggc cagtggttta gcggttgcga ttgcgttggc ggcttgcgca
    64081 gataaaagcg cggatattca gacgccagca ccggctgcaa atacgtctat ttcagcaaca
    64141 caacaacctg ctatccagca accgaatgtc tccggtaccg tttggatccg tcagaaagtc
    64201 gcactgccgc ctgatgccgt gctgaccgtg acactttctg acgcgtcgtt agccgatgcg
    64261 ccgtcgaaag tgctggcgca gaaagcggtg cgtaccgaag gtaaacagtc accattcagc
    64321 tttgttctgc catttaaccc ggcagatgtt cagccgaacg cgcgtattct gttgagtgcg
    64381 gcgattaccg tgaatgacaa actggtattt attaccgata ccgttcagcc ggtgatcaac
    64441 cagggcggaa ctaaagccga cctgacattg gtgccggtgc agcaaaccgc cgtgccggtt
    64501 caggctagcg gtggcgcaac gactaccgta ccttcaactt caccaaccca ggtgaatccg
    64561 tcttcggcag ttccagctcc tacgcaatat taagcgaagt taaccctctc cgcccggaga
    64621 gggttagtag ttccagcgat aaagctgcaa gtcgatttgc ccgcttcccg acaccatcac
    64681 accttctgcc agtaatgcct gtcgctgacg ctgtaaatcc ggtccggtca gtgaaatggt
    64741 gccgtggcga ttaaccaccc ggtgccaggg caaggtgctg ccttcgggga gacgctttaa
    64801 cacaccgcct acctggcgcg cggcgcgcgg cgatcccgcc agtttcgcca catcaccgta
    64861 agtggtgaca tagccttcgg gaatagcggc tacgatttgc cagacgcgtt ggggaaatga
    64921 atcttctttt tccatctttt cttcctgagg taatttttca gcataatctg gaaaaacgcc
    64981 cgagtgaagc cgcattgcgc aagaaaccag catctggcac gcaatgggtt gcaattagcc
    65041 ggggcagcag tgataatgcg cctgcgcgtt ggttctcaac gctctcaatg ggggctctgt
    65101 tggttctccc gcaacgctac tctgtttacc aggtcaggtc cggaaggaag cagccaaggc
    65161 agatgacgcg tgtgccggga tgtagctggc agggccccca cccatttctg cctcccaccg
    65221 tttcgtcaaa aaatcccaac atggctaaac tttaaccaca actgacgtcg caagaattgt
    65281 ctggctgcgc agtacgcttc ggaggtatgt ctgatgaagt atgttgatgg ttttgttgtt
    65341 gccgttcctg ccgataaaaa ggatgcctat cgggaaatgg ccgctaaggc cgcgccatta
    65401 tttaaagagt tcggtgcgct tcgtattgtc gaatgctggg ccagcgatgt accggatggt
    65461 aaagtgaccg attttcgtat ggcggtgaaa gcggaagaga atgaagaggt tgtctttagc
    65521 tggattgaat acccttcaaa agaggtccgc gacgctgcta atcaaaagat gatgtcggac
    65581 ccacggatga aagagttcgg cgagtccatg ccgtttgacg gcaagcgaat gatctatggt
    65641 ggattcgaat caatcatcga cgagtaggta gcgtgacggg ctgtgcggcg tgccgtcagc
    65701 cctaaaattc acaaatgttg ctcggcccag cgtaagaaat cctctttcgg taatgccttg
    65761 ctgtagagcc agccctgacc gtagtgcacg ccatgctggc gtagccactc ttcttgttta
    65821 ctggtttcga ttccctccgc aaccattttc agtttcagtg tttttgccat ttcgatgata
    65881 tgcggcgtga catttttata ttccagcgca tcaacgaaag atttatcgat cttcagaatg
    65941 tcgacatcca gatcctgtaa atagcttaaa cttgaatacc ctgtaccaaa atcatcgaga
    66001 taaatttcat ggcccgcctc ccggtagcga gaaattatcg gggcgctggt tttcggatcg
    66061 gcaaactcgc gttcagtgag ttcaagcgcg atctgtctgg gattaacctg atagtgattg
    66121 atcatttcac gcagcaattg cgggattttt tccgaggtga gcacggtgga ttcaagattg
    66181 atcgaaatat gctgctgaga atgctgacgc agccagtcgc ccatatcttc aaagacgctt
    66241 cttataatca gtagcgtcaa tggctcagaa aggcccgttt gctgtgccag cggaataaaa
    66301 ctatctggtg acaaccaact accgtctgtc tgcggccagc gcgccagtgc ctcagcaccg
    66361 acaattttgc cattggctaa ggagacaatc ggctgatagt gcacgcaaat atcacgattt
    66421 tcgatagcat cctgcagccg atgatgcggt gactggatac ggcgcaaaat acgcagcaca
    66481 aacatcgctg ccagcaggcc aatcaccaac ccgagcggta accaaataaa gacttgccga
    66541 tgccagcctt tctgcaacat tttcgttgaa gcccaggtga tgatcgaaat attcatctcc
    66601 ggtaagggca ggatatcgta aatgattcca ttattttcga tatgctcacc gggtgttttt
    66661 tgtagcctgg taataattcc ctgagcaatt ttatcgctgc tggttatgac aacgttatgg
    66721 gcattgccaa taatggcggc atcaatttgc catgagctat agggaatgac atcaataaag
    66781 gaagcggggt cgatcatgac gacataatgt gccgttccca tggcgaccat gtaacgaata
    66841 atgcctaaat cgttatgcga tgttaaccag acacgataac catctttcga aattttacct
    66901 ggctcgggga aggtatcggg cgggctttca tgctccagag acgaacattg gggaacgttg
    66961 ttatcgatat aaaccacttc ctgaatatag cgataactgt aagatacccg acgcatttcc
    67021 atgagatggg cttcgctaca ggcagcgcct tgccatcttt ccagctcttg cagcgcatct
    67081 ttcccttgtg tcgccacctt attggcccga atagcgacgc gggaggaata cgtattcagc
    67141 tcttcaataa atgatgtttc tacctgctga tgggccagcc agatgcttaa gccaacaggc
    67201 agcaatactg aaagaatcag tactcccgaa ataaggccga ccagatgtcg tgttctcacc
    67261 agcatatcct tattaacaca gtgtagggaa agtatatctg attgtgaaaa aaggctggca
    67321 ggagataagg aggtagagct ggtatttcat gaagtgatgg gtggaagcga gcgttgtcat
    67381 ttgaccaggc gtagagcttt acgcctggta tgtaccccta acttaagatg ttgattcggc
    67441 gcgagcgagg gtaaaaatat cgtaaaaaga tagttcacct ttacggacga tcattttttg
    67501 cagttgttct ttatgaacat ccgcgacacg cggagagttc aggatggctt caaccagcgc
    67561 ggcaggtaca aagcgcgtgt tgtaccattc gccgttatag caaacgcgga aatccagcac
    67621 gtcgatatct tcatagcggt aacgtgggta gacatcgaaa aagaagaaac cattaagcag
    67681 gataaataat acaaccagta gccagacaga gcctgaaagc gtttcggatt gcaacatcac
    67741 cgccagggta gcaaaaaaag caacgtacat accgataaac agccccggat gtttacggat
    67801 aaaactgata ctaaagcggg gtttgttgtc gcgtttttcg cgggtattca gagactcaat
    67861 cgtttcggtc agcaggcgtt gtatttcggt catcgtttgc cttcgtgata tctgcaactt
    67921 tccagggaac accctatttt aacgtgggaa tagtagaggg gaagtaacag atttgtcacg
    67981 attttgcata acagttgaga aaagctggtt acaatttttt aatgattctg gctggattac
    68041 cgcccacgac aacgttgtcc gggacatctt ttgtgacaac tgcacctgag gctaccacga
    68101 cgttatcacc aatggtcaca ccagggttaa tgaccgcacg tccgccaatc cagacgttat
    68161 taccgatggt gacgggtttc cccagttcag caccgctatt acgtgctaca gggtcgatgg
    68221 gatgtgttgc cgtgtagata tgaacgcctg gtgccaacat acagttatca ccaatgcgaa
    68281 tagggcagac atcaagcatc acgcaatcga agttggcgaa aaaattattg ccgagaaaaa
    68341 tgttataacc atagtcacag cgaaacgttg gctcaatata agcctctgtc acctgaccga
    68401 ataaatcagc aagaatttgc tggcgtaatg tgtgctcttc cgccagggaa tgattgtatc
    68461 ggtgaataag ccgacgagcg cgcaggcgat cgcgagataa cgcctcatct gccgagtgat
    68521 acaactcacc agcaatcatc ttttcttttt ctgtgctcat tactgacctc cggttgctga
    68581 aaatgcggca acacagtaag acgaaaagtc agtgaagaga acgttcccat tacatctgtg
    68641 tgatgaagat cacatagggt atgccttcat ggcgaaaaaa ttataaaact cttaataaac
    68701 agccggttat agctccgaaa gcgagttatt tattagcgaa taaatttcca tactgaggaa
    68761 gggatcttgt cgtacagttt attcatggtc aattcggcga ggcggtgatc tgcggctgag
    68821 taaaataccg ccagttcatt atctgataat tcgtatttat ttttctcgat aacacgctcc
    68881 agcgtgtcaa ttgtctgaca acgacgtaaa cgcattaaat aatcggtttt cgttaaaggt
    68941 ttttcggaca tacttctacc catggttgta ataattctaa caagataaac tcgcaggatt
    69001 ctctttcgtc gcattgacaa aacaacggaa tagtcgatta cctgacttcc gccatttctg
    69061 aagatcctgc atattaatac cataactact gaacaacata aaggtgtcat ccagatattc
    69121 gtcgatctgc tcaatgagct tattgtcttc attatactta attttgtaat taagtgcgaa
    69181 ggtcgcaata tgctcaatca gttcattcaa ctggaggttg atcgccgagg ttgggtcgtt
    69241 aacccagcca tgattgcttt cttcaaggtt tgcaaggcag tcatgataca gggtttcaca
    69301 gagaaactta agctgtgcga tatcatgtct tttgggtgag tattcatcca taacgcgtcc
    69361 ccttcttagc ggttgaacta acggacacct ttcgggatgg aaaaaactta ctgacctgga
    69421 cttgccctct cgtttgttag cttaactata agccactctt tgtaggtttt catcgcatta
    69481 ttacgaaaaa ttacaattag gaaggaagcg ggacaggact cactttttca tactagcacc
    69541 aacccgcagc aggtaaaagc agtttgctcg aactgttcat aacatttcag aattatcgcc
    69601 agaagaaatt ggtgacacta ataccaggat tgctctgaat atgacgtaat aaccgaggaa
    69661 tgaataaaga attaccgcaa ataattaaga atagcctcta aatggttatg gattattaaa
    69721 tctattagcg ctacttaata taaattaact aacaatcagt attttttatg aattttatac
    69781 gtggttaata ctggttttcg tatgagatcc tgagttggtg gttcaatact ccttaatgtt
    69841 cgtaggttat gcataaaaaa ggccgcttgc gcggccttag tgattacacg ttgtatcaat
    69901 gatgatcgac agtatggctg tgctcgatat cttcattctt gcggctaaag cggcggcgaa
    69961 ccaccacaaa gaataccgga acgaagaaga ttgccagtac cgttgcggtc accatcccgc
    70021 ccattacacc ggtacctact gcgttctgcg cgccggaacc agcaccagta ctgataacca
    70081 gcggcataac gccgaggata aacgccaacg aggtcatcag gattgggcgt aaacgcatcc
    70141 gcaccgcatc aagcgtcgct tcaatcagac ctttaccttc tttatccatc aagtctttgg
    70201 cgaattcgac gataagtatc gcgttcttcg ccgacaaacc aatggttgtg agcaggccta
    70261 cctggaagta aacgtcattg gtcaggccac ggaaggtggc agccagcaac gcaccgataa
    70321 cccccagcgg aacgaccagc ataacggaga acggaatcga ccagctctcg tacagcgcag
    70381 ccagacacag gaacacgaca atcaacgaaa tcgcgtacag tgaaggtgcc tggttgccgg
    70441 agagacgttc ctgataggac attcccgtcc agtcatagcc aacaccggta ggcagtttgc
    70501 tcgccagttg ttccatcagc tccattgctt caccggtact tttacccggt gccgcctggc
    70561 ctaagatttc catagatggc aggccgttgt aacgttccag acgcggcgaa ccgtactccc
    70621 aacgagaaga ggagaacgcc gagaacggca ccatctgacc atcagcagca cgaacatacc
    70681 agtcgccgat atcatccggc agcatacggt atttcgcttc tgacatgacg taaactttct
    70741 tcacacgacc gcggtcgata aagtcgttca catagctacc gccccatgca gcgcccagag
    70801 tggtgttaat gtcgttgata gaaacaccca gcgcctgcgc tttttcctgg tcgatatcaa
    70861 tcttaaactg cggggtatct tccagaccgt ttggacgtac gctggtcaac atatcagggt
    70921 gcttcgctgc ttctgcaagc aactggttac gcgcctgagt cagtttttcg tgaccaaggc
    70981 cagcctggtc aatcagctca aagtcaaagc cggttgcggt acccagttcc acgattgcgg
    71041 gcaggttaaa ggcgaaaacc atcgcatctt tgatttgcga gaaagcacgt gttgcacgca
    71101 tggtaatcgc ttcaactttg ttttcttcgc ccggacgatc ggcccagtcc ttcaaggaaa
    71161 cgaacgcaat accggtattc tgaccacgtc ccgcaaagcc gaagccgtta acggcgaaca
    71221 ccgactcaac gttgttcttt tctttggtca gatagtaatt cgttacctca ttgagcactt
    71281 tctgcgtacg ttcctgcgtt gcacctgctg gcagctgaac catggtcata aatacgccct
    71341 ggtcctcatc tggcaagaag gagcttggca gacgcacgaa cagataggcc ataccgacca
    71401 cgatgatcag atacagcacc aggtaacgcc ccgtactgcg cagaataccg cctacgctgt
    71461 cggtgtagtg gtgcgtgctc ttctcgaaca tgcggttaaa ccagccgaag aagccttttt
    71521 taccttcccc gtgatcgcct ttggcaatcg gtttcagcat ggtggcacaa agagccggag
    71581 tcaggatcaa cgccaccagt accgacagcg ccattgctga aacaatggta atagagaact
    71641 gacgatagat agcaccagta gaaccgccaa agaaggccat cggtacgaat accgccgaca
    71701 gtaccatcgc gataccgacc agagcgccct gaatctgccc catcgactta cgggtggctt
    71761 cttttggcgg caaaccttct tccgccatca cacgctcaac gttttctacc acaacgatag
    71821 cgtcatccac caacaggccg atggcgagca ccatcccgaa cattgttagc gtgtttatcg
    71881 agaagccaaa ggcggcaagg acggcaaagg tcccgagcaa taccaccggt acggcaatgg
    71941 tcggaatcaa cgtcgcgcgg aagttctgca agaacagata cattaccagg aacacgagga
    72001 tgatcgcttc gaccagcgtt ttaaccactt cgtgaataga gattttcacg aacggtgtgg
    72061 tgtcatacgg gtaaacaatt ttcagacccg acgggaagaa cggttccatc ttcgccagtt
    72121 cagcacggat tgccgcagcg gtatccagtg cgttcgcacc ggtcgccagc ttgatcccca
    72181 gaccggaagc cggttggccg ttaaactctg cgatgatgtc atagttctca ccacccagct
    72241 caattttcgc cacatcacgc agcagcacgc gggaaccatc ctgattcact ttcagcagga
    72301 ttttgccgaa ctcttcagta gaggtcagac gcgtctgagc aataatagag gcgttaagct
    72361 gttggccttt caccggcggc gtaccaccga gctgaccagc tgcaacctgg gcgttctgtg
    72421 ctttgatggc ggtaatgaca tcaaccggcg ttaactggaa tttgttcagt tcattcgggt
    72481 tcatccagat acgcatcgcg tactgtgaac cgaacaactg aacgtcaccc acgcccgacg
    72541 tacggctgat ggcatctttc atattcgccg ccacgtagtc ggagatatcc tcctgcgtca
    72601 tggtgccatc ggtgttgata acgccgacaa ccatcaggaa gctgctggat gatttctcaa
    72661 cgctcacccc ttgctgctgt acttcttgcg gcagcaacgg catcgccagc tgcagtttgt
    72721 tctgcacctg aacctgcgcg atatccgcat cagtaccaga ctcaaaggtc agggtgatct
    72781 gcacggtacc cgtggagtca ctgttagagg acatgtacat caggttatcg ataccgttca
    72841 tattctgttc gataacctgt gtcaccgtgt cctgcactgt tttcgcatca gcgccagggt
    72901 aggaggcgga gatcgttact gccggcggtg caatcgtagg atattgcgcc accggcagtt
    72961 tgaggatcgc cagcccccct gccaacatga tgataatggc gatcacccac gcaaaaatcg
    73021 ggcgatcgat aaagaaatta ggcatgtctt aacggctcct gtttaagtta agacttggac
    73081 tgttcaggct gagcaccgct tgcggcttgc tggttattat cagcggtaac ttcttgtgct
    73141 tttacctgga caccaggacg cactttctgc agcccactta ttactacgcg atcgcctgct
    73201 ttcagacctt ctgtcaccag ccacttatcg ccgatagcct ggcttgcaac gatcggacgg
    73261 gtttccactt tgtcatccgc gccaaccacc agtacggtgg catcgccacg cggcgtacgg
    73321 gttacgccct gttgcgggac taaaatagcg tttggattaa gcccttcttc cagacgtgca
    73381 cgcacgaaca tacccggcag cagagtgtga tccgggttcg ggaagatagc gcgtagggtg
    73441 atagacccag tggtctgatc aacggtaacg tcagagaatt ccagcgtacc gtcctgcggg
    73501 aacttaatgc cgtcactggt gatcagcgac actttggctt tgccgttctc ttgtttcagc
    73561 gtgccattcg ccagttcctg tttcaggcgc aggaagtcgt tgctggactg ggtcacatca
    73621 acgtagatcg gatcaagttg ctgcacggtt gccagcgcag tcgcctgacc gttctgtacc
    73681 aatgcgcctt ccgtcacgtt tgacttacca atgcgaccac taatcggaga ggtgactttg
    73741 gtgtaagcca gattgattcg cgaagtttca acggcagctt tcgccgcagt taccgcagca
    73801 ttcgcctgtt gcgcatcagc cagagcctga tcgtactctt gcttactgat gtactgagta
    73861 ccgagcaatt tctgataacg attcaccgtc aattgcgcga tattggctgc agcctgggct
    73921 ttcgccagat cacctttcgc actgtcgtat gccgcctgat aggtcgcagg atcaatctga
    73981 tagagagaga cacctgcttc gatgtcgcta ccttctttga aattacgctt caggataatc
    74041 ccgctaactt gaggacgaac ttctgcgatc cggtaggcac tggtgcgacc cggaagctcg
    74101 gttgtgatct gcagaggttc agttttgact gttactacgc caacggcggg catctgctgg
    74161 ccaccttgtt gggcctgttt gtcgtcacat cctgttaggg ctaagctgcc tgagagcatc
    74221 agaacgaccg ccagaggcgt aaaccctctg tttttgttca tatgtaaacc tcgagtgtcc
    74281 gatttcaaat tggtcaatgg tcaaaagtta ataaacccat tgctgcgttt atattatcgt
    74341 cgtgctatgg tacatacatt cacaaatgta tgtaaatcta acgcctgtaa attcacgaac
    74401 atatggcacg aaaaaccaaa caagaagcgc aagaaacgcg ccaacacatc ctcgatgtgg
    74461 ctctacgtct tttctcacag cagggggtat catccacctc gctgggtgag attgcaaaag
    74521 cagctggcgt tacgcgcggt gcaatctact ggcattttaa agacaagtcg gatttgttca
    74581 gtgagatctg ggaactgtca gaatccaata ttggtgaact cgagcttgag tatcaggcaa
    74641 aattccctgg cgatccactc tcagtattaa gagagatatt aattcatgtt cttgagtcca
    74701 cggtgacaga agaacggcgt cgattattga tggagattat attccacaaa tgcgaatttg
    74761 tcggcgaaat ggctgttgtg caacaggcac aacgtaatct ctgtctggaa agttatgacc
    74821 gtatagaaca aacgttaaaa cattgtattg aagcgaaaat gttgcctgcg gatttaatga
    74881 cgcgtcgcgc agcaattatt atgcgcggct atatttccgg cctgatggaa aactggctct
    74941 ttgccccgca atcttttgat cttaaaaaag aagcccgcga ttacgtcgcc atcttactgg
    75001 agatgtatct cctgtgcccc acgcttcgta accctgccac taacgaataa ccctgaatct
    75061 gactccagga attttcctgg acattttcgt cgttgctatt ctggttcact gcgtcgtgat
    75121 attcttgcgg tttgactttt tcaggtcgtt cttcaggttc agaaaccttc attcatcatg
    75181 actatgttcc agtattacaa acgatcacgg cattttgttt tttcagcatt tattgctttt
    75241 gtttttgtct tgttatgcca gaacacggcg tttgcacggg cgtcatcgaa tggtgattta
    75301 ccgacaaaag cggacctgca ggcacaactt gactcactga ataaacaaaa agatctttct
    75361 gctcaggaca aactggtgca gcaggatctg acagatacat tagccaccct cgataaaatc
    75421 gatcgcgtaa aagaagagac agttcagcta cggcaaaaag tcgctgaagc gccggaaaaa
    75481 atgcgccagg cgaccgcggc gttaacagca cttagcgatg tcgataacga cgaagaaacg
    75541 cgcaaaattc tgagtacgct ttcgttgcgc cagctggaaa ctcgcgttgc ccaggcgctg
    75601 gacgatttgc agaacgcaca aaacgatctg gcgtcttata acagccaact ggtttcgcta
    75661 cagacgcagc ccgaacgcgt acaaaatgcg atgtataacg cttcgcagca gttgcaacaa
    75721 attcgcagtc gtctggatgg gactgatgtc ggcgagactg ccttacgtcc cagccagaaa
    75781 gtgttaatgc aggtccagca ggctttgctg aatgccgaga ttgaccagca gcgtaaaagc
    75841 ctggaaggga acactgtctt gcaggatacc ttgcaaaagc agcgtgatta cgtgacggcg
    75901 aacagcgccc gtctggagca ccagttacaa ctgttgcaag aagcggtaaa cagcaagcgc
    75961 ctgactttaa ccgaaaaaac ggcgcaggaa gctgtctccc cggatgaagc cgcgcgtatt
    76021 caggctaatc cgctggtgaa gcaggaactg gaaattaacc agcagttaag tcagcgtctg
    76081 attaccgcga ctcaaaacgg taatcagttg atgcagcaaa acattaaagt caaaaactgg
    76141 ctggagcgtg cgctgcaatc ggaacgtaat attaaggagc agattgccgt tctgaagggc
    76201 agtctgctgt tgtcacgtat cctgtaccag caacaacaaa cactcccctc ggcagatgaa
    76261 ctggaaaaca tgaccaaccg catcgcggat ttgcgtctcg aacagtttga agtcaaccag
    76321 cagcgtgacg cgctgttcca gagcgatgcg ttcgtcagca aactggaaga aggtcacacc
    76381 aacgaagtca acagcgaagt tcacgatgca ttattgcaag tagttgatat gcgtcgcgaa
    76441 ttgctggatc agcttaacaa acagttgggt aaccagctga tgatggccat taacctgcaa
    76501 atcaaccagc agcagttaat gagtgtgtcg aaaaacctga aatccatcct gacgcagcaa
    76561 attttttggg tgaacagtaa ccgcccgatg gactgggact ggattaaagc gttcccgcaa
    76621 acgctgaaag atgaatttaa gtcgatgaaa atcacggtga actgggaaaa agcctggccc
    76681 gccgtattca tcgccttcct cgctggtttg ccgctgctgt tgattgccgg gctgatccac
    76741 tggcgtctgg gctggctgaa agcgtatcaa caaaaactgg cttccgctgt gggttccctg
    76801 cgtaacgaca gccagctcaa cacaccaaaa gcgatcctta tcgacctgat ccgcgcactg
    76861 ccggtgtgcc tgattattct cgctgtcggc ctgattctgc tgactatgca gctcaatatc
    76921 agcgagttat tgtggtcgtt cagcaaaaaa ctggcgattt tctggctggt atttggcctg
    76981 tgctggaaag tgctggagaa aaatggtgtt gccgtgcgtc actttggtat gccggaacag
    77041 cagaccagcc actggcgtcg gcaaattgtc cgcatcagcc tcgcgttgct gccgatccat
    77101 ttctggtctg tggtggcaga actctccccg ctgcatctga tggatgatgt gctggggcag
    77161 gcgatgattt tcttcaacct gctgctgatt gccttcctgg tgtggccgat gtgccgcgaa
    77221 agctggcgtg acaaagagtc gcacaccatg cgactggtca ccattaccgt gctgtcgatt
    77281 atcccgattg cgttgatggt gctgaccgcc acgggttact tctacaccac gctgcgcctc
    77341 tccgggcgtt ggattgaaac cgtttatttg gtgatcatct ggaacctgct gtaccagaca
    77401 gtattgcgcg gcttaagcgt agcggcgcgg cgtatcgcct ggcgtcgtgc actggcgcgt
    77461 cggcagaatc tggtgaaaga gggcgcagaa ggtgccgaac cgccggaaga acccaccatt
    77521 gcgctggagc aggttaacca gcagacgctg cgtattacca tgttgctgat gtttgcgctg
    77581 ttcggtgtca tgttctgggc aatttggtct gatttgatca ccgtgttcag ctatctcgac
    77641 agcattacgc tctggcatta caacgctact gaagctggtg ctgcggtggt gaaaaacgtc
    77701 accatgggca gtctgttgtt tgcgattatc gcctcaatgg tggcctgggc gttgattcgc
    77761 aacctgcctg gtttgctgga agtgctggtg ctctcgcgac tgaatatgcg ccagggcgcg
    77821 tcgtatgcca ttactaccat ccttaactac atcattattg ccgttggtgc gatgacggtg
    77881 ttcggatcgc tgggcgtctc gtgggataaa ctccagtggc tggcggcagc attatccgta
    77941 ggtcttggtt ttggtttaca agagatcttc ggtaacttcg tttccggttt gatcattctg
    78001 ttcgaacgtc cggtgcgcat aggcgacacg gtaaccattg gtagcttctc gggtacggtc
    78061 agcaagatcc gtattcgtgc gacaacgatt accgatttcg atcgcaaaga agtgattatc
    78121 ccgaacaaag cgtttgttac cgagcgtctg atcaactggt cgttaactga cacgactacg
    78181 cgtctggtga tccgtctcgg cgtagcctat ggctccgatc tggaaaaagt gcgtaaagtg
    78241 ttgctgaagg cggcgactga gcacccaagg gtgatgcacg aaccaatgcc ggaagtcttc
    78301 tttacggcat ttggtgccag cacgttggat catgagctgc gtctgtatgt gcgtgaactg
    78361 cgtgaccgta gccgtactgt cgatgagctg aaccgtacta tcgatcagtt gtgccgtgaa
    78421 aacgacatca acattgcctt taaccaactt gaagtgcatc tgcacaacga gaagggcgat
    78481 gaagtgacgg aagtgaaacg cgactacaaa ggcgacgatc cgacgccagc ggtagggtaa
    78541 taaccaaagg gggcgacatt tagttgcccc gagattacta acaaagtgcg cgttgttcat
    78601 gccggatgcg gcgtgaccgc cttatccggc ctacgaaacc gcaagaattc aatatattgc
    78661 aggagcggtg taggcctgat aagcgtagcg catcaggcag ttttgcgttt gcccacaatc
    78721 ttaggggaca tttagcggcc ccatttattt ctcacttttc cgcctcatca tcgcgcgtta
    78781 atttcttttc ataatcgcgt tttacaatat ccagcgcgcg cagcacggtg cgggctggga
    78841 tctgattttc ttccagtagc acaatcaaat cgacggccag tttgacatcg tcaggggcat
    78901 tttccagtga catactctct ccattgctag cgggttaaac gcgctaacct gttttcgatt
    78961 ttttccagcg catggcggca gcgtgccagg cgcgcttcat aggcttccac ttcacgatgc
    79021 agcgtctgct gttccacgag atcggtcgct cgtgccagac gggctctacg ttcggcaacc
    79081 atctcacgta gccgccgctc aaaatcctga tgctgaatac gtttacgctg ccagcgggca
    79141 attttcggtg gtgcgctatc ccactcgcgt agcgaccagg ctgtggcttc acgcgctata
    79201 gcctccagtt gcgccgccag atgttctgcc agccaggcga cttgcggcag ttgttgctgc
    79261 tcaaccgcat gacgaagcgc agccaggtta tcgcccgcct catcgagaca agcctgtagt
    79321 gttgtcgcac gggtctgaaa aaggtgcctg tcaaaacggg cacttagcgt ggcaaattgc
    79381 gctaccgggg cacaacgctg acgcagcgtg gcgagctgtc cttccagttt ttccagcagc
    79441 agggcggttt tcacgataag acctcaatga aaatgataat tgttatgcta aagtagccgc
    79501 tctgtaagct gacaacactc aatatgcaac gaatcatttt aatcatcatt ggctggctgg
    79561 cggtagtgct gggtacgctg ggcgtggtat taccggtatt accgacgacg ccgtttatcc
    79621 tgctggcggc ctggtgcttt gcccgttctt ccccgcgctt tcacgcctgg ttgctgtacc
    79681 gctcatggtt tggcagctat ctacgtttct ggcagaaaca tcatgcgatg ccgcgcggcg
    79741 caaaaccacg ggcgattttg cttattttga ttacgtttac gatttcgctg tggttcgtcc
    79801 agatgccgtg ggtgcgcatc atgttgctgg taattctcgc ctgtttgctt ttttatatgt
    79861 ggcgaattcc ggtgattgat gaaaagcaag aaaagcactg aagcacaaca atcgcagttg
    79921 caattattgc gtacagccag tacattctgg cgttttcgag cacaggcgca ggcggtcaaa
    79981 ggttaaacaa ctgttacttt tgatacgttt aaaacgcgcc gtgagtacca ccgtaacaag
    80041 caggcataca cttatgaccg cgactgcaca gcagcttgag tatctcaaaa atagcatcaa
    80101 aagcattcag gactacccaa aacccggcat tcttttccgc gatgtcacca gcttactgga
    80161 agacccgaaa gcttacgctc tcagcatcga cttgctggtt gagcgttaca aaaatgcggg
    80221 cattaccaaa gttgtcggca ccgaagcgcg tggcttcttg tttggcgctc cggtagctct
    80281 gggtctgggc gttggctttg taccggtccg taaaccgggc aaactgccgc gtgaaaccat
    80341 cagtgaaact tacgacctgg aatacggcac cgatcagctg gaaatccacg ttgatgccat
    80401 caaaccgggc gacaaagttc tggtggtgga cgacctgctg gcaaccggcg gcactatcga
    80461 agcgaccgtt aaactgatcc gtcgtctggg tggtgaagtg gctgatgctg cgttcattat
    80521 caacctgttc gatctcggcg gcgaacagcg tctcgaaaaa cagggcatta ccagctacag
    80581 ccttgttccg ttcccgggcc attaattatc gccagtcttg tgctgcccac gctacggaca
    80641 gcacaagatg tgcattcagc ctcgccgttc tgacggggct gtgttagcat taccccttcg
    80701 tgaatccacc ttccagcgtt tcagagcctg ccaatgagtt atcaggtctt agcccgaaaa
    80761 tggcgcccac aaacctttgc tgacgtcgtc ggccaggaac atgtgctgac cgcactggcg
    80821 aacggcttgt cgttagggcg tattcatcat gcttatcttt tttccggtac ccgtggcgtc
    80881 ggaaaaacct ctatcgcccg actgctggcg aaggggctaa actgcgaaac cggcattacc
    80941 gcgacgccgt gcggcgtgtg cgataactgt cgtgaaatcg agcaggggcg ctttgtcgat
    81001 ctgattgaaa tcgacgccgc ctcgcgcact aaagttgaag acactcgcga cctgctggat
    81061 aacgtccagt acgctccggc gcgtggtcgt ttcaaagttt atctgatcga cgaagtgcat
    81121 atgctgtcgc gccacagctt taacgcactg ttaaaaaccc ttgaagagcc gccggagcac
    81181 gttaagtttc tgctggcgac gaccgatcca cagaaattgc cggtgacgat tttatcgcgc
    81241 tgtctgcaat ttcatctcaa ggcgctggat gtcgagcaaa ttcgccatca acttgagcac
    81301 atcctcaacg aagaacatat cgctcacgag ccgcgggcgc tgcaattgtt ggcacgcgcc
    81361 gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc aggcgattgc cagcggtgac
    81421 ggccaggttt caacccaggc cgtcagtgcg atgctgggta cgcttgacga cgatcaggcg
    81481 ttgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc gcgtaatggc gctaattaat
    81541 gaagctgctg cccgtggtat cgagtgggaa gcgttgctgg tggaaatgct cggcctgttg
    81601 catcgtattg cgatggtaca actttcgcct gctgcacttg gcaacgacat ggccgccatc
    81661 gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga cggatattca gctttactat
    81721 cagacgctgt tgattggtcg caaagaatta ccgtatgcgc cggaccgccg tatgggcgtt
    81781 gagatgacgc tgctgcgtgc gctggcgttc catccgcgta tgcctctgcc tgagccagaa
    81841 gtgccacgcc agtcctttgc gcccgttgcg ccaacggcag taatgacgcc aacacaggtg
    81901 ccgccgcaac cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    81961 agccaggtgc tggctgcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    82021 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    82081 ctggcttcgg tcaccgatcg cgttcaggcg cgcccggtgc catcggcgct ggaaaaagcg
    82141 ccagccaaaa aagaagcgta tcgctggaag gcgaccactc cagtgatgca gcaaaaagaa
    82201 gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    82261 gcggcgaagc tggcggcaga agccattgag cgcgacccgt gggcggctca ggtgagtcaa
    82321 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    82381 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggcgcacag
    82441 caaaaactgg ctgaagcgtt gagcacgtta aaaggttcaa cggttgaact gactatcgtt
    82501 gaagatgata atcccgcggt gcgtacgccg ctggaatggc gtcaggcgat atacgaagaa
    82561 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcga
    82621 ttcttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    82681 acgttcgtta tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    82741 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    82801 cgcgcagctg gaagtcaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    82861 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    82921 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    82981 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    83041 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgcctgccg
    83101 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    83161 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    83221 gattgccgca ccttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtaag
    83281 gaaaacggtc aaatctgcgt ggtggagagt ccggcggaca tctacgccat tgagcagacg
    83341 gggcagtttt caggccgtta ttttgtgttg atggggcacc tgtcaccgct ggacggcatc
    83401 ggtccggatg atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    83461 gaagtgatcc tcgccaccaa ccccacggtt gaaggtgaag ctaccgctaa ctacattgcc
    83521 gagctttgtg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    83581 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact cccttgccgg gcgtcataag
    83641 attcgttttt aagcaaacga gagcaggatc acctgctctc gcttgaaatt actctccctt
    83701 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    83761 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    83821 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    83881 cgatgcggcg gacaagctgc gtttccgcgc gctttctaac ccggacctgt acgaaggtga
    83941 tggtgaacta cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    84001 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    84061 cggcaccaaa tcattcctcg aatccctggg ttctgaccag gcgaaagaca gccagctgat
    84121 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gctgacaaag tgaccgtgcg
    84181 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    84241 tgaatacacc gttgccgaca tcactaaaga agatcgtggt actgaaatca ccctgcatct
    84301 gcgtgaaggc gaagacgagt tcctcgatga ctggcgtgtg cgttccatca tcagcaaata
    84361 ttccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag acggcgaaac
    84421 cgttatctcc tgggagaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    84481 aatcaccgat gaagagtaca aagagttcta caaacacatc gcccacgact ttaacgatcc
    84541 gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat
    84601 tccgtctcag gcaccgtggg atatgtggaa ccgcgatcat aaacacggtc tgaaactgta
    84661 tgttcagcgt gtgttcatca tggacgacgc agaacagttc atgccgaact atctgcgctt
    84721 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    84781 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    84841 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    84901 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    84961 gcgttttgct tctacccata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    85021 ttcccgcatg aaagaagggc aggagaaaat ctactatatc accgcagaca gttacgcggc
    85081 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    85141 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    85201 ccagtctgtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    85261 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgtgtgaaag ccctgctcgg
    85321 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    85381 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    85441 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    85501 ggcggatact gaagatgaag cgaagttcag cgagtgggta gaactgctgc tggatcaggc
    85561 gctgctggca gaacgcggta cgctggaaga tccgaacctg tttattcgtc gtatgaacca
    85621 gctgctggtt tcctgatgtc atgccggat
//
LOCUS       DAZKHK010000004        84536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKHK010000004 DAZKHK010000000
VERSION     DAZKHK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.004"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(16..231)
                     /locus_tag="ACHOS5_000243"
     CDS             complement(16..231)
                     /locus_tag="ACHOS5_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9775137.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
     gene            complement(294..1766)
                     /gene="der"
                     /locus_tag="ACHOS5_000244"
     CDS             complement(294..1766)
                     /gene="der"
                     /locus_tag="ACHOS5_000244"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9775138.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWVQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1884..3062)
                     /gene="bamB"
                     /locus_tag="ACHOS5_000245"
     CDS             complement(1884..3062)
                     /gene="bamB"
                     /locus_tag="ACHOS5_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP9775139.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISLATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3073..3693)
                     /gene="yfgM"
                     /locus_tag="ACHOS5_000246"
     CDS             complement(3073..3693)
                     /gene="yfgM"
                     /locus_tag="ACHOS5_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP9775140.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3711..4985)
                     /gene="hisS"
                     /locus_tag="ACHOS5_000247"
     CDS             complement(3711..4985)
                     /gene="hisS"
                     /locus_tag="ACHOS5_000247"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9775141.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5096..6214)
                     /gene="ispG"
                     /locus_tag="ACHOS5_000248"
                     /gene_synonym="gcpE"
     CDS             complement(5096..6214)
                     /gene="ispG"
                     /locus_tag="ACHOS5_000248"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP9775142.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6241..7248)
                     /gene="rodZ"
                     /locus_tag="ACHOS5_000249"
     CDS             complement(6241..7248)
                     /gene="rodZ"
                     /locus_tag="ACHOS5_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP9775143.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7533..8687)
                     /gene="rlmN"
                     /locus_tag="ACHOS5_000250"
     CDS             complement(7533..8687)
                     /gene="rlmN"
                     /locus_tag="ACHOS5_000250"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP9775144.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8837..9268)
                     /gene="ndk"
                     /locus_tag="ACHOS5_000251"
     CDS             complement(8837..9268)
                     /gene="ndk"
                     /locus_tag="ACHOS5_000251"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9775145.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9417..11729)
                     /gene="pbpC"
                     /locus_tag="ACHOS5_000252"
     CDS             complement(9417..11729)
                     /gene="pbpC"
                     /locus_tag="ACHOS5_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP9775146.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLSLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGSWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAAATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11730..16691)
                     /gene="yfhM"
                     /locus_tag="ACHOS5_000253"
     CDS             complement(11730..16691)
                     /gene="yfhM"
                     /locus_tag="ACHOS5_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP9775147.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNILQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16898..17743
                     /gene="sseA"
                     /locus_tag="ACHOS5_000254"
     CDS             16898..17743
                     /gene="sseA"
                     /locus_tag="ACHOS5_000254"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP9775148.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYMN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVNCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            18083..18208
                     /gene="timP"
                     /locus_tag="ACHOS5_000255"
     CDS             18083..18208
                     /gene="timP"
                     /locus_tag="ACHOS5_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP9775149.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSFSGNNLLVVNKLQSSK"
     gene            complement(18240..19016)
                     /gene="sseB"
                     /locus_tag="ACHOS5_000256"
     CDS             complement(18240..19016)
                     /gene="sseB"
                     /locus_tag="ACHOS5_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP9775150.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEGEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19159..20442)
                     /gene="pepB"
                     /locus_tag="ACHOS5_000257"
     CDS             complement(19159..20442)
                     /gene="pepB"
                     /locus_tag="ACHOS5_000257"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP9775151.1"
                     /translation="MTEAMKITLSPQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGIRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRNQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20501..20701)
                     /gene="iscX"
                     /locus_tag="ACHOS5_000258"
     CDS             complement(20501..20701)
                     /gene="iscX"
                     /locus_tag="ACHOS5_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP9775152.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20713..21048)
                     /gene="fdx"
                     /locus_tag="ACHOS5_000259"
     CDS             complement(20713..21048)
                     /gene="fdx"
                     /locus_tag="ACHOS5_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP9775153.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21050..22900)
                     /gene="hscA"
                     /locus_tag="ACHOS5_000260"
     CDS             complement(21050..22900)
                     /gene="hscA"
                     /locus_tag="ACHOS5_000260"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP9775154.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDAVSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQAIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22917..23432)
                     /gene="hscB"
                     /locus_tag="ACHOS5_000261"
     CDS             complement(22917..23432)
                     /gene="hscB"
                     /locus_tag="ACHOS5_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP9775155.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23528..23851)
                     /gene="iscA"
                     /locus_tag="ACHOS5_000262"
     CDS             complement(23528..23851)
                     /gene="iscA"
                     /locus_tag="ACHOS5_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP9775156.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23868..24254)
                     /gene="iscU"
                     /locus_tag="ACHOS5_000263"
     CDS             complement(23868..24254)
                     /gene="iscU"
                     /locus_tag="ACHOS5_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP9775157.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24282..25496)
                     /gene="iscS"
                     /locus_tag="ACHOS5_000264"
     CDS             complement(24282..25496)
                     /gene="iscS"
                     /locus_tag="ACHOS5_000264"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9775158.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25608..26096)
                     /gene="iscR"
                     /locus_tag="ACHOS5_000265"
     CDS             complement(25608..26096)
                     /gene="iscR"
                     /locus_tag="ACHOS5_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP9775159.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26367..27107)
                     /gene="trmJ"
                     /locus_tag="ACHOS5_000266"
     CDS             complement(26367..27107)
                     /gene="trmJ"
                     /locus_tag="ACHOS5_000266"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP9775160.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27226..28029
                     /gene="suhB"
                     /locus_tag="ACHOS5_000267"
     CDS             27226..28029
                     /gene="suhB"
                     /locus_tag="ACHOS5_000267"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP9775161.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28174..29028
                     /gene="yfhR"
                     /locus_tag="ACHOS5_000268"
     CDS             28174..29028
                     /gene="yfhR"
                     /locus_tag="ACHOS5_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9775162.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29219..30499
                     /gene="csiE"
                     /locus_tag="ACHOS5_000269"
     CDS             29219..30499
                     /gene="csiE"
                     /locus_tag="ACHOS5_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP9775163.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTANTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQHLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30491..31630)
                     /gene="hcaT"
                     /locus_tag="ACHOS5_000270"
     CDS             complement(30491..31630)
                     /gene="hcaT"
                     /locus_tag="ACHOS5_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP9775164.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            31999..32421
                     /gene="yphA"
                     /locus_tag="ACHOS5_000271"
     CDS             31999..32421
                     /gene="yphA"
                     /locus_tag="ACHOS5_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9775165.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNISIAGAFLLLAITGPGAISLDRR"
     gene            complement(32581..33453)
                     /gene="yphB"
                     /locus_tag="ACHOS5_000272"
     CDS             complement(32581..33453)
                     /gene="yphB"
                     /locus_tag="ACHOS5_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9775166.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRVQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLTPLPHQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33465..34559)
                     /gene="yphC"
                     /locus_tag="ACHOS5_000273"
     CDS             complement(33465..34559)
                     /gene="yphC"
                     /locus_tag="ACHOS5_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9775167.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVVVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGMPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34592..35590)
                     /gene="yphD"
                     /locus_tag="ACHOS5_000274"
     CDS             complement(34592..35590)
                     /gene="yphD"
                     /locus_tag="ACHOS5_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9775168.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFSVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35615..37126)
                     /gene="yphE"
                     /locus_tag="ACHOS5_000275"
     CDS             complement(35615..37126)
                     /gene="yphE"
                     /locus_tag="ACHOS5_000275"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9775169.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLKNTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(37149..38132)
                     /gene="yphF"
                     /locus_tag="ACHOS5_000276"
     CDS             complement(37149..38132)
                     /gene="yphF"
                     /locus_tag="ACHOS5_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9775170.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38229..41510)
                     /gene="yphG"
                     /locus_tag="ACHOS5_000277"
     CDS             complement(38229..41510)
                     /gene="yphG"
                     /locus_tag="ACHOS5_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP9775171.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSDRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIILSYQEHQPQVLPLPDVAKA
                     PLAAQDITSTDETWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDTGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVVKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41628..42821
                     /gene="yphH"
                     /locus_tag="ACHOS5_000278"
     CDS             41628..42821
                     /gene="yphH"
                     /locus_tag="ACHOS5_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9775172.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42885..44138)
                     /gene="glyA"
                     /locus_tag="ACHOS5_000279"
     CDS             complement(42885..44138)
                     /gene="glyA"
                     /locus_tag="ACHOS5_000279"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9775173.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44466..45656
                     /gene="hmpA"
                     /locus_tag="ACHOS5_000280"
     CDS             44466..45656
                     /gene="hmpA"
                     /locus_tag="ACHOS5_000280"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP9775174.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVDPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45701..46039)
                     /gene="glnB"
                     /locus_tag="ACHOS5_000281"
     CDS             complement(45701..46039)
                     /gene="glnB"
                     /locus_tag="ACHOS5_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP9775175.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46100..47434)
                     /gene="glrR"
                     /locus_tag="ACHOS5_000282"
     CDS             complement(46100..47434)
                     /gene="glrR"
                     /locus_tag="ACHOS5_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP9775176.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47424..48137)
                     /gene="qseG"
                     /locus_tag="ACHOS5_000283"
     CDS             complement(47424..48137)
                     /gene="qseG"
                     /locus_tag="ACHOS5_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP9775177.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(48302..49729)
                     /gene="qseE"
                     /locus_tag="ACHOS5_000284"
     CDS             complement(48302..49729)
                     /gene="qseE"
                     /locus_tag="ACHOS5_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP9775178.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50305..54192)
                     /gene="purL"
                     /locus_tag="ACHOS5_000285"
                     /gene_synonym="purI"
     CDS             complement(50305..54192)
                     /gene="purL"
                     /locus_tag="ACHOS5_000285"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP9775179.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVRRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHVL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54450..56006
                     /gene="mltF"
                     /locus_tag="ACHOS5_000286"
     CDS             54450..56006
                     /gene="mltF"
                     /locus_tag="ACHOS5_000286"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP9775180.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNTSQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTTKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(56003..56506)
                     /gene="tadA"
                     /locus_tag="ACHOS5_000287"
     CDS             complement(56003..56506)
                     /gene="tadA"
                     /locus_tag="ACHOS5_000287"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9775181.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(56564..57199)
                     /gene="yfhb"
                     /locus_tag="ACHOS5_000288"
     CDS             complement(56564..57199)
                     /gene="yfhb"
                     /locus_tag="ACHOS5_000288"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP9775182.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQSLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57408..58256
                     /gene="yfhH"
                     /locus_tag="ACHOS5_000289"
     CDS             57408..58256
                     /gene="yfhH"
                     /locus_tag="ACHOS5_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9775183.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58312..58572
                     /gene="yfhL"
                     /locus_tag="ACHOS5_000290"
     CDS             58312..58572
                     /gene="yfhL"
                     /locus_tag="ACHOS5_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP9775184.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(58766..58846)
                     /gene="shoB"
                     /locus_tag="ACHOS5_000291"
     CDS             complement(58766..58846)
                     /gene="shoB"
                     /locus_tag="ACHOS5_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP9775185.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(59267..59647)
                     /gene="acpS"
                     /locus_tag="ACHOS5_000292"
     CDS             complement(59267..59647)
                     /gene="acpS"
                     /locus_tag="ACHOS5_000292"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9775186.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(59647..60378)
                     /gene="pdxJ"
                     /locus_tag="ACHOS5_000293"
     CDS             complement(59647..60378)
                     /gene="pdxJ"
                     /locus_tag="ACHOS5_000293"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP9775187.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVCILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLADAGIQVSLFIDADEDQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(60390..61118)
                     /gene="recO"
                     /locus_tag="ACHOS5_000294"
     CDS             complement(60390..61118)
                     /gene="recO"
                     /locus_tag="ACHOS5_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9775188.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(61130..62035)
                     /gene="era"
                     /locus_tag="ACHOS5_000295"
     CDS             complement(61130..62035)
                     /gene="era"
                     /locus_tag="ACHOS5_000295"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9775189.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(62032..62712)
                     /gene="rnc"
                     /locus_tag="ACHOS5_000296"
     CDS             complement(62032..62712)
                     /gene="rnc"
                     /locus_tag="ACHOS5_000296"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9775190.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(62984..63958)
                     /gene="lepB"
                     /locus_tag="ACHOS5_000297"
     CDS             complement(62984..63958)
                     /gene="lepB"
                     /locus_tag="ACHOS5_000297"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9775191.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(63974..65773)
                     /gene="lepA"
                     /locus_tag="ACHOS5_000298"
     CDS             complement(63974..65773)
                     /gene="lepA"
                     /locus_tag="ACHOS5_000298"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9775192.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(65970..66449)
                     /gene="rseC"
                     /locus_tag="ACHOS5_000299"
     CDS             complement(65970..66449)
                     /gene="rseC"
                     /locus_tag="ACHOS5_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP9775193.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(66446..67402)
                     /gene="rseB"
                     /locus_tag="ACHOS5_000300"
     CDS             complement(66446..67402)
                     /gene="rseB"
                     /locus_tag="ACHOS5_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP9775194.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFNWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            complement(67402..68052)
                     /gene="rseA"
                     /locus_tag="ACHOS5_000301"
     CDS             complement(67402..68052)
                     /gene="rseA"
                     /locus_tag="ACHOS5_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP9775195.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(68085..68660)
                     /gene="rpoE"
                     /locus_tag="ACHOS5_000302"
     CDS             complement(68085..68660)
                     /gene="rpoE"
                     /locus_tag="ACHOS5_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP9775196.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(68657..68812)
                     /gene="rseD"
                     /locus_tag="ACHOS5_000303"
     CDS             complement(68657..68812)
                     /gene="rseD"
                     /locus_tag="ACHOS5_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP9775197.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            69068..70690
                     /gene="nadB"
                     /locus_tag="ACHOS5_000304"
     CDS             69068..70690
                     /gene="nadB"
                     /locus_tag="ACHOS5_000304"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP9775198.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRIANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(70675..71412)
                     /gene="trmN"
                     /locus_tag="ACHOS5_000305"
     CDS             complement(70675..71412)
                     /gene="trmN"
                     /locus_tag="ACHOS5_000305"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP9775199.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATSQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            71544..72878
                     /gene="srmB"
                     /locus_tag="ACHOS5_000306"
     CDS             71544..72878
                     /gene="srmB"
                     /locus_tag="ACHOS5_000306"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP9775200.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(72911..73792)
                     /gene="yfiE"
                     /locus_tag="ACHOS5_000307"
     CDS             complement(72911..73792)
                     /gene="yfiE"
                     /locus_tag="ACHOS5_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9775201.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVISGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLALVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            73895..74491
                     /gene="eamB"
                     /locus_tag="ACHOS5_000308"
     CDS             73895..74491
                     /gene="eamB"
                     /locus_tag="ACHOS5_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP9775202.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSASSATSHGFRQSTR
                     VLAGMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDG
                     LQTKPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGN
                     VCWALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(74545..74928)
                     /gene="grcA"
                     /locus_tag="ACHOS5_000309"
     CDS             complement(74545..74928)
                     /gene="grcA"
                     /locus_tag="ACHOS5_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP9775203.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            75233..75922
                     /gene="ung"
                     /locus_tag="ACHOS5_000310"
     CDS             75233..75922
                     /gene="ung"
                     /locus_tag="ACHOS5_000310"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9775204.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(75970..77007)
                     /gene="yfiF"
                     /locus_tag="ACHOS5_000311"
     CDS             complement(75970..77007)
                     /gene="yfiF"
                     /locus_tag="ACHOS5_000311"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9775205.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            77214..77633
                     /gene="trxC"
                     /locus_tag="ACHOS5_000312"
     CDS             77214..77633
                     /gene="trxC"
                     /locus_tag="ACHOS5_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP9775206.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            77702..78400
                     /gene="tapT"
                     /locus_tag="ACHOS5_000313"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             77702..78400
                     /gene="tapT"
                     /locus_tag="ACHOS5_000313"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP9775207.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            78432..81092
                     /gene="pat"
                     /locus_tag="ACHOS5_000314"
     CDS             78432..81092
                     /gene="pat"
                     /locus_tag="ACHOS5_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP9775208.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIARLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDTLPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAHALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHIVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIVEGATSLDTHEVQPILQAYGMNTLPTWIATDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     NEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVALRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            81206..82561
                     /gene="pssA"
                     /locus_tag="ACHOS5_000315"
     CDS             81206..82561
                     /gene="pssA"
                     /locus_tag="ACHOS5_000315"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP9775209.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            82607..82930
                     /gene="yfiM"
                     /locus_tag="ACHOS5_000316"
     CDS             82607..82930
                     /gene="yfiM"
                     /locus_tag="ACHOS5_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP9775210.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(82927..84225)
                     /gene="kgtP"
                     /locus_tag="ACHOS5_000317"
     CDS             complement(82927..84225)
                     /gene="kgtP"
                     /locus_tag="ACHOS5_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP9775211.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPIIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYTAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
BASE COUNT        20164 a        23126 c        21034 g        20212 t
ORIGIN      
        1 tacattcaca tgatattaag caataataaa ttcaacgcgt tttttagata ttaaaccgtg
       61 cccatgttgg cagaaataat ccacagcacc acaagctttt aacacttgta acgcctgatg
      121 acactcaggg caaagtgcct ggacggtaaa gtctttgtcg caagttgcac agtgagcagt
      181 ctcgccattg cgttcgagag gggttttgca ggttggacag ttgagtgcca taaatactcc
      241 tcgcgtgtta ttcggcttcc ctttccgtgt ggaaagggaa accagataag tgattactta
      301 cttttcttga tatgcttcat cagacgctta cgtttacgca tctgggttgg cgtcagggtg
      361 ttacgcttgt tcgcatacgg gttttcccct tctttgaact gaatacgaat cggcgatccc
      421 attacgtcca gcgatttgcg gaagtagttc atcaggtaac gcttgtagga atcaggcagg
      481 tctttcacct gattgccgtg aatcaccaca atcggcgggt tataaccccc ggcgtgggcg
      541 tatttcagct tcacacgacg accgcgtacc agcggtggtt ggtgatcttc aacagccatc
      601 gtcatgatgc gcgtcagcat agaggtcccc acacgacggg tggagctgtc atacgcttca
      661 cgtactgatt caaacaagtt accaacacca ctgccgtgca aggcagagat aaagtgcaca
      721 cgagcaaaat cgataaagcc cagacggaag tccagcgtct ctttcacctg ctctttcact
      781 tcctggctca ggccgtccca cttgttcact acaatcacaa gtgagcgccc actattgaga
      841 ataaagccca gcaaggagag atcctgatcg gaaatacctt cacgcgcatc aatcaccaac
      901 atcaccacgt tggcatcttc aatggcctgc aacgttttga ttaccgagaa tttctctacc
      961 gcatcggtga ttttgccgcg tttacgtacg ccagcggtgt caatgagcac atactcacgt
     1021 ccgtcgcgtt ccattggaat gtagatactg tcacgcgtcg tgccaggcat gtcgtaaaca
     1081 acaacgcgct cttcaccaag aatacggtta gtgagtgtag acttacctac gttcgggcga
     1141 cccacaatcg ccagtttgat cggcagactt tgcgggtcga agtcgtcttc ctcttcttct
     1201 tcgccgttct cttccgcttc aaattgcacc cagtattcgg cgtcttcgtc gacttcttct
     1261 tgcggtgcga gatcttccat ccacggcagc agcacgtgct ccagcagact taatacgcca
     1321 cgaccgtgag acgcggcgat cgggtagatt tcacctaaac caagcgcgta gaaatcaacc
     1381 actgcctgat cgggatccag accgtcagtt ttgtttgcca ccaggaaggt cggtttttca
     1441 cgggagcgca gatgtttagc aatcgcttca tccgccggca tcaggcccgc gcgcgcatcc
     1501 accataaaca gtacgacgtc cgcttcttca atcgccagca gcgactgttc cgccatgcgg
     1561 gtttctacac cgtcttctgt gccatcaatc ccgccggtat caatgcagat aaactcacgg
     1621 ccttcaattt ccgcacgacc gtacttacgg tcacgagtca gacccgggaa atccgcaacc
     1681 agcgcatctc gggtgcgagt tagacggtta aataacgtgg attttcctac gttagggcgc
     1741 ccgacaagcg cgaccacagg taccatgttt aaagcctcat ttttataaat catcagacaa
     1801 cgcacgctat attcgtgtcg ttgttaaaaa caggaaaacg gcccctgtcc aggagccgtt
     1861 ttcaaagtga acgacagaga cgattaacgt gtaatagagt acacggttcc gtcttttgcc
     1921 tggatcagca gtttgccgtc agcagcaacc ggttcagtct ggaaaccgga actatcaact
     1981 ttttgctggg caacgaaacg accatcttcg acgttaatcc agtgcagata accttcactg
     2041 tcgcctacca ccaggttgcc attatacagc accggagaag tcagcaggcg atgcagcaga
     2101 tcgctttgtg tccacagcgt aacgccgcca tcaatggtca acgccatcac ccggtcattt
     2161 tgatcgacca gatagatgcg attgccgtcg acgatgaaat cattcaccga acccagttcg
     2221 cgtttccaca taatctgacc gctgcgcaga tcaagcgccg tcaggttacc attataggcc
     2281 agcgcgaaaa caacgccgtt aacaacgacg ggagtcgtgt caacatcgct cagacggtca
     2341 atttcggtag aaccggtcgc cagggaaata cgctgctgcc aaatcatctg gccctgttcc
     2401 atcagcactg cgctgacgcg accattatcg ccacccacga cggccgcacc aaaagccgtt
     2461 gccggcgcag actcgccacg caaagagagc gaaggcatat cgaggttaac tgtccatttg
     2521 acagcgccgt cagcttcgtt cagcgcttgt aactgaccgt tactggtgtg gattaacacc
     2581 agaccgtcgc tgaccaccgg gcgcgaaagt gcttcacccg cgactttagt ttgccatgcc
     2641 acagtaccat cgctggtatt cagcgcgtaa acctgcgcct tttcggtgcc aatgtagaca
     2701 tgcccaccag acacggtcac gccgccagaa agtaatgcag gatctttaga gaaccagcca
     2761 tctttctcgg ccaggttgac agaccagatt tctttgccgt catccgcatt cagcgctttt
     2821 actaaaccag cgcggtccgc tgcatagaca acgttgtccg ccagtgccgg atgaagattg
     2881 gaatagaagt tgccaatgcc gctaccaacg gaagtgctcc acacagtagt cggcgtaaac
     2941 tggttttcaa cggttggcaa tggggacatc tttaccacat cttcttcgct gttaaacagc
     3001 gaacagccgc ttaaaagggt aacggaaagc agtcctggca gcagtaattt acgcaattgc
     3061 atcgggtccc tctcagatgg acaaattatt aattttcatc tgcatcattt cgctcagtgc
     3121 cggggtaaca tcgcttttca cgcctgcttc ccatgcacta cgcgcacctt gcttatcacc
     3181 tttgctcagc aatgcttcac cacgcaggtc ggcaacaatg gcagcccacc cttcaccttt
     3241 gatggtatca agggttttta acgcggcatc agcctgcttg agctgtacct gaacgcgagc
     3301 aagacgcaga tttatcacgg ctttgagatt ttcatcgctc gtgtctgcca gcccctgttg
     3361 taactgggcg gcagctttct ctagttcatt tttgtcaaca aattgctgcg caagttccaa
     3421 agaagccagc gcaccataag tatttttatt ttcagcagca aatttttccg ccgctgggat
     3481 gctatccggt ttgccttcgc tcactgcggt aaccgcattt tgataggcaa gagaagcgga
     3541 gcgtgcagaa tcaacctgat ggctgttcca gtagcgccag ccaatcagtg cgccaacgcc
     3601 caaaatcacc ccaacagcca gtgctttgcc attttcagca aaaaagcgtt taaccgcttc
     3661 aacctggtcg ttttcgttct cgtaaatttc cacgctgtcc ttctccttcc ttaacccagt
     3721 aacgtgcgca aatgcgcggc tacgctatcc tgcgcaactg cagtttgctc accagagcgc
     3781 aaatccttca ctactgctgt gccgttagcc acttcagact cacccagcac cacagcaacg
     3841 cgggcacccc atttatcagc acgggcaaac tgtttcttaa agttgccgcc gccgtggttg
     3901 gtcatcagtt tcacgcccgg taattcatca cgcagacgct ctgctaatgc catagccgca
     3961 gattgtgtgt cagcacctga agccaccagg tatatatcga caacaggatc ggctttaaat
     4021 tccggattaa cggcctgaac taacaataca agacgttcga ggcccatcgc aaaaccgact
     4081 gccggtgttg cacgaccacc cagttgttcc accagaccgt cataacgacc acctgcacac
     4141 acggtgccct gggaaccgag gctgttagtc acccactcga aaacggtacg gttatagtaa
     4201 tccagaccgc gcaccagacg ctggtttacg gtgtaagcga tccccgcgct ctcaagcagt
     4261 ttgcacagac cggcaaagtg ctcacgagat tcctcgtcca gataatcgcc taatgccgga
     4321 gcgtcgttga gaagcgcctg cacttccgga ttttttgaat ccagtacgcg caacgggtta
     4381 gtgtacatgc ggcgtttgca gtcttcgtcc agcttttctt tatgctgctc aaggaatgcc
     4441 accagcgcat cgcggtaatt cgcacgagct tccagcgaac cgatagagtt cagctcaaga
     4501 gttacgtgct cggaaatacc cagcgcgcgc caccagcggg cagtgagcat aatcagttcg
     4561 gcgtcgatat ccggaccttg cagaccgaaa acttcgcagc ccaactgatg gaactgacga
     4621 taacgccctt tctgcggacg ctcgtgacgg aacataggcc cgatatacca cagacgctgt
     4681 tcctgattgt acagaagacc atgctcgatg ccggcgcgta cacagcccgc cgtcccttca
     4741 gggcgcagag tcaggctgtc gccattgcga tcctcaaagg tgtacatctc tttttcaacc
     4801 acgtcggtga cttcaccaat cgcacgtttg aatagcgggg tctgctctac aatcggcaag
     4861 cggatttcac tgtaaccgta gctgccgagc acgtttttca gtgtgccttc aatgcgctgc
     4921 cagatggccg tttcgccagg caggtaatcg ttcatgccgc gaatggcttg aatgtttttt
     4981 gccacgttta ttctctttct gaatataaaa atgaaccctc aacgcttccc tcaatgtttc
     5041 gggagccatg cgggttcaat catacacggg aagcgaggcg cttcccatca cgttattatt
     5101 tttcaacctg ctgaacgtca attcgacgcg cttcgtccag ctgactggct ttcgcacgaa
     5161 tgcgcgcttc cagctgatcg atcatatcgt tgttgtccag acggtctttg cgcacgccat
     5221 cttcatagag gccgcttttc ttgttgccgc cagtgacgcc gagtgtagaa accagcgcct
     5281 caccagggcc attcaccacg cagccgataa tcgaaacgtc catcggagtg atgatatctt
     5341 ccaggcgttg ctccagcgcg ttaaccgtac cgataacatc aaattcctga cgcgaacagg
     5401 tcgggcaggc gatgaagttg atcccgcgcg aacggatacg cagcgatttc aaaatatcga
     5461 aaccgacttt gatctcttcg accggatcgg ccgccagcga tacgcgcagc gtgtcgccga
     5521 tgccttcaga cagcagcaga cctaaaccaa tagcggattt tactgccccg ctacgcgcac
     5581 caccggcttc ggtgatcccc agatgcagcg gctgatcgat ttgttttgcc agcaaacgat
     5641 aagactcaac agcgaggaag acgtcagacg ctttcacgct gactttgaac tgatcgaagt
     5701 tcaggcgatc gagatgatca acatgacgca tagcagattc cagcaacgcc tgcggcgtcg
     5761 gttcgccata cttttcttgc agatcttttt ccaacgatcc ggcattaacg ccaatacgga
     5821 tcggaatgtt tttatcgcgc gcacagtcaa ccaccatgcg aatacgctct tcattaccga
     5881 tattgccagg gttaatacgc agacaatcga cgccgtattc cgctactttc agcgcaatgc
     5941 gatagtcgaa gtggatgtca gccaccagcg gcacgttaac ctgctgtttg atgagtttga
     6001 acgcttctgc cgcgtccatc gtcggtacgg agacacggac gatatcagcg ccaacacgtt
     6061 ccagcgcctt gatttgattg accgttgctt cgacgtctgt cgtacgcgta ttggtcatgg
     6121 actgtacggc gatgggagca ccatcgccaa tcggcacatt cccaacgtaa atacgtgttg
     6181 attttctacg ttgaattgga gcctggttat gcatgaaaaa tctcccgcgt tacccgtctg
     6241 ttactgcgcc ggtgattgtt cggcattgag ggtcagacgc gcaacctggt tagttctgat
     6301 aaaacgactc agatcgacag gtttcccttg atactggatc tgtactgcgg ctggcgcacc
     6361 aattttcagt ttgtacggtg cctggccggt taagttcaaa ttaccgtctt tacgctgcat
     6421 accgctaaac agttttttac cggtagcatc ggtgacttcc agccagcaat cggcagtaaa
     6481 gttcatcacc agcgcattcg ggtcaaccgc cggcgtggtc acgccagcct gatcggttgg
     6541 caacggcgca gcgccatctg gtgttgttgt tgccgctggt gccggagtcg cagcggtatc
     6601 aacatttgcc tgcgaaggtg gaacaaccgc attctgttgc ggatcaacag ctggtgctgg
     6661 cgcagttacg gcaggtgttt gcgtgttggt tgcggtggta tccacaggtg atgtcggcgt
     6721 ggtcgccata gcctgatctg tggttgtcga cgtatctaac ggtacactct ggctttgatt
     6781 attattcagt tccgcagaag attgatcggc catagtggtg atctcttcct gctgagcttt
     6841 gtggtcttgc caccaccagg caccgctcag accaataacc acaaacaaca ccagccaggt
     6901 gaaggtcatc agccagccgt cacgtttttt gcggcgttta ccgagggaaa aactctgcat
     6961 cggcgcaact tttgcagccc gtagtggagc ctgcttttcc agccctggca gcagttcttc
     7021 ttctggaata tgtaccagac gcgcataaga gcggatatat ccgcgcagga atgttgaagc
     7081 aagatcggca ggtgccttat cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag
     7141 tcgctcggca acggcctgtt gactaagtcc tagttgttcg cgagcattac gcaggcgagc
     7201 gccggtagta agtgcttcat tttggtcgtg cgtggcttca gtattcattc gctgcaggta
     7261 cgtttaaatg agaattgaga tgccggtgaa ccatcatgcc cacccacacc gcgaaacatc
     7321 cgttaagtta accgttatca tacagtataa gaccgtctgt gtgctcgtga caaagcccac
     7381 attcgctaag gctaacttac tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg
     7441 caccgttaat attgaccaga caattgcaac ttaatacttc attcataaaa gattacgcca
     7501 cggtacataa agtaaccgtg gcgtaatggc tatcagaccg ctttaatgtc gatggcttca
     7561 ccctgcatcc gcttacgcag agtacgtttc gtacggtcga taacatcgcc cgccaactga
     7621 ccacaagcag catcgatatc atcaccacga gttttacgca caatagtggt gaaaccgtag
     7681 ctcatcagca cttttgagaa acggtcgata cggctgttcg aactgcgtcc atacggcgcg
     7741 cccgggaacg ggttccacgg gatcaggttg atcttacacg gcgtatcttt cagtagttcc
     7801 gccagttggt gcgcgtgttc agtgccgtcg ttaacgtggt caagcatcac gtattcaata
     7861 gtgactcggc cctgattggc gttggatttc tccagataac ggcgcaccgc cgcaaggaac
     7921 gtttcgatat tgtacttttt gttgatcggc acaatctcgt cacgaatttc gtcgttcggc
     7981 gcgtgcaggg aaattgccag cgctacgtcg atcatatcgc ccagtttatc cagcgccgga
     8041 accacaccgg aagtggaaag cgtgacgcga cgttttgaca ggccaaaacc gaaatcatca
     8101 agcatgattt ccatcgccgg aacgacgttg ttcaggttga gcagcggctc gcccataccc
     8161 atcatcacta cgttagtgat tgggcgctga ccggtgactt ttgctgcgcc gacaattttc
     8221 gccgcacgcc acacctggcc gataatttcc gatacccgca ggttgcggtt aaagccctgc
     8281 tgggcggtgg aacagaattt acactccagc gcacacccca cctgcgaaga gacgcagagc
     8341 gtggcacggt cgtcttccgg gatatacacc gtttcaacgc gctgatcgcc aacggcgatc
     8401 gcccatttaa tggtgccgtc agatgaacgc tgttcttcaa ccacttccgg tgcacggatt
     8461 tccgccacct ctttcagttt gccgcgcaac actttattga tgtcggtcat ctcatcaaag
     8521 ttgtcgcagc aatagtgata catccacttc atcacctgat cggcgcggaa gggtttttca
     8581 cctaaatctt taaaaaactc ccgcatctgc tgacggttga gatccagcag gttgattttt
     8641 ccatctttcg tggtgacgtt ttcaggtgtg actaattgtt cagacatatg ctattccggc
     8701 ctcgttatta cacgttatgg cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc
     8761 tgctcgtccg ggggcgctgc attgtacaaa ttctggcgca cggatgccac gtttgcacgc
     8821 ggcatttacg aaattattaa cgggtgcgcg ggcacacttc gccttcgccg aagaaataag
     8881 cgatttcgcg agcggcagac tcgacggaat cagaaccgtg ggtaccattt tcggtcaggc
     8941 tgtcagcgta atcagcacgc agagtaccgg ccagtgcgtt tgccggattg gtcgcgccca
     9001 gcagatcgcg gtgacgctga acagcatttt caccttccag cacggaaaca acgatcgggc
     9061 cagaagtcat gaattcaacc agaccatcaa agaacggttt tccatcgtgt tcagcataaa
     9121 agccacgagc ctgttcaacg gtcaggtgca gcattttggt gccgacaatt ttgaaccctg
     9181 cagcttcaaa gcgcgcaaag atactaccaa tcacgttttt tgctaccgcg ttcggtttga
     9241 tgatggaaaa agtacgttca atagccattt ttacctctgt aaattgttct gttgttgtct
     9301 gtaccagcgt acgagatggc gcggattata atgagcaaca gggccgttga ctattgatga
     9361 aggtaacatt tttttaaaat aaacccagtt taagcaacaa tcagggacaa aatagactat
     9421 tgcatgacaa atttcactgt cgctatttgc cccacgtcat ccatcaccag caattgataa
     9481 tcgcctttat ccgtcaaatg cagggtcacg ttgcgcccac gttcagttaa cggttcgccg
     9541 ttcagaaacc accagcgttc acctgctcct ccacttgatt gcaacggcaa agtcgctgcc
     9601 gctgcgcccg gtaaacgttt aataatcgcg ccatcgcgca cacctgttag ctgcagcggg
     9661 agttgtgcat cgtgaccgta tggcgggcaa attgtcgagg ccggtggcaa gcgcacagcg
     9721 cgacgttctg atgcgggcag ccagggttcc agcggtagcg gccagacatt tatcatttct
     9781 tgtcgcgcct gagggcaatc agcggcaaca cgtttgccgt tttcatccag ccagatgggg
     9841 aaacgaatgc cattaatgcc ttcctgctcc ggcagtaata gagttggcgg ctggctcccg
     9901 tccagcagcc aggttgccag gcggcggcga cagttaccgt caccttccgg caaagactgt
     9961 ccgcccggcc agcagataac gccacgactt acagagtcag gacgtgggtc ttccggcaaa
    10021 ttcacactgc gcgacagtaa gatattatta acctgattca acagcggtac ggcactggca
    10081 aagccaaact gaccaacaac gggcgtgccg tccggtctgc cagtccagct cccaatgaca
    10141 tagcgagcgt taaccccaat cgcccaggca tcacgatagc catagctggt gcccgttttc
    10201 catgccagtg gggcgacgcg cggcaaggca ccatccggca agggttgcgc ttcatcagcc
    10261 ataatccggc gaatgatcca cgcagccccc gacgacatta aaggccgttc aagcagcgga
    10321 tcgtcaggct gtaagcgcaa tttacctgcc ttgccgtggc gagcaaacgc ggtatacgct
    10381 gccgccatat cttccagttt tgcgccagcg ccgccgagaa tgagcgaaag attcggtgca
    10441 gcaccgttgg gcaaatataa cgacaatcca acattgcgta actttgccgc aaaccgtttc
    10501 ggtccatagg cttccagcac ctgcacagca ggtaagttca gcgatcgcac cagcgcctca
    10561 ctcatgctga tcgggccatg aaaaccgctg tcaaagttgc caggtcggta atcaccggtg
    10621 cgtctgggga cgtcttgcaa cagcgatgcc gggtggatca agccttcatc cagcgccaga
    10681 ccataaacaa acggtttgag caccgatcct ggcgatcgga tcgcattgac catatcaaca
    10741 tgaccaaagc gtgaatcatc gttgagatca accgatccca cccagccgcg aacacgcata
    10801 tcggtatgat caaccacgat catcgccagt gagctgcgcg gtggcaatcg tcctttccag
    10861 ttttgcgcca gttcttccag acgtcgttga agaccggcat ccagcgtagt aacgattttg
    10921 tcgcttttgc ttttaccgag catcatgcgc gaaaacagcg gtgccagttg cggcatttgt
    10981 cggggtgcca gccagatggg ttcttccctt gactctttta cccgctcacg ggaccacaca
    11041 ccttgcacgg ccatccgttc gagcacttta ttacgcgcgg cttcggcacg ctccggccaa
    11101 cgatccgggc gaagacggct gggcgcttgc ggcaacaccg ccagcattgc cgcctcggaa
    11161 tagcttaaat tcgcaggcga tttcccgagg taagcccaac ttgccgcacc aatcccctgc
    11221 aacgtaccgc caaacggggc acggttaaga tacaaggtca gaatttcacg tttagacaga
    11281 tgccattcca gttgcaacgc gcgccagagc tggcgaattt tgccgccaaa cgttttgggg
    11341 tgaggatcaa gcaggcgcgc cacctgcatg gtgagcgtgc tgccgccgga aatgacccga
    11401 cccgacgtga gatcttgcca ggctgcacgc gccaccgaga acggattcac ccccggatgc
    11461 ttccagaacc agcgatcttc ataattgatc agcgcttcaa ggtaacgtgg agaaacatct
    11521 tcgattgtta ctggataacg ccagatgccg tcagcatcgg cgaaacgcca gagtggcgta
    11581 ccatcctgcg ccaccaccac gcgtgcagga ttcacttctt gcaatggcag aggccagagt
    11641 ttatctgctg cccatgctgc cagaatgatg ataaaaggcg cggctgccag cattatccag
    11701 cagccgcgtt tggttaacag gcgaggcatt tacggtctga ctatcaacag atcgtcagcc
    11761 gcaccagtcg cccgccattg aggaacatac attgattcca ccataggctg cggcacctga
    11821 tacgttcccg gcgtcaccgc ccgcgccaga tacaccagcg ttaccggttg atattcatca
    11881 acggcaaccg ccgccacaaa gcgatcgtca cggaactcta tgtgcttaat gctcgcctgc
    11941 tgcatctggt tcagcaagtt ctgcacttca ccaccacttt gctccaggct ggcgctgccg
    12001 ttcgccagat tctggttttc cagttccagc cccgcaggca acagatccac gactaacgca
    12061 tccggtacac tgttactggc tttaacctgc aaccacacca gcaccagatc gccgctacgt
    12121 aacgagtcca gcgatttgct cttaccgtca gtacccagaa tatgacgctc aatttgcagc
    12181 acattgctcg ccggtaaagg tgcggattgc ggataaccgc tggcatccac acgcagccat
    12241 aacggctgat cgccactgtt ggtcacctgc aaggtggcaa gttgatcgct attcagattg
    12301 ctggtttgcg ctttctcgcc tgtcaacggc tcagcagaga aagtggtttg cgcctgccat
    12361 ttgcccggta aatcctgaag agtacgggca gccaggaaca acgcgttact ttcctgcgtc
    12421 gatagccagc gttcaccgaa cgcctgctgc gaaagtgtgt ttaacaggga gtactgctca
    12481 tcgggtagca gtttgttctc ttccagcaat gagagcatca acgcgctgtc tcgcagtgga
    12541 ctaccgtaat cacccagcca tatccgctca tcactattac gcggcgtttt cagcgccagc
    12601 gcaatcgctt cttcaccacg cgtcgcatca cccatgattt tcagcgcaac gccaagttgc
    12661 agcagcggta aaccagaagc ggcatctgca cgatgctccc agatttcacg cagcgcaccc
    12721 agtggtgctt tttgctgacg ggccaacacc agcgcagcgt aagactgtac ggcgaattta
    12781 ctggctttga gattatcagc gtacgggatc gacatcatgc ccggatcttg taaatagcgc
    12841 agcagacgct cattaccccg gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct
    12901 gcgcggacca ggaaatccat cacgtaagcc gtcagccagt actcttcgtc accgttttta
    12961 tcccacaacg caaagccgcc gttatcacgt tgcatttgca gcaaacggga aatgccgata
    13021 tcgaccgatg cacggcgttt ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg
    13081 gctgcattgg tataaagtga cgggaacagg ccgctggcgg tttgctcgag acagccgtac
    13141 ggatacgctt ttaactcttt gatataacgt gcgatgttca gcggtggttt gccactcaac
    13201 aacaattgcc cttccagcgt aacaggcgag aagttttgca atccatcagc cgggagcgcc
    13261 caggtttcac caggctgtaa cgccgtaccg taattgaccg tttgtgccgg gaatgccgga
    13321 cggacgccga ttttccactg cttatgctga tcggcaacgg tttcccccgg taacgctaac
    13381 ccgctaatgg tggcctgaat gtctccatcg ccataacccg gcaatgcacg caccgggata
    13441 aacagcgtag tacgcacccc tggcgctaat tcaacaggtg cgggggaatt actcaccagt
    13501 tcaagcaagc cactggcggt cagagcaaca ttaagttttt gcggtttatc ggtaagatta
    13561 gtgatatcca gcgtcaggcg cgaggtatcg ccactcgcca taaagcgcgg catgttcagt
    13621 tcagcaatca ccggtgcggc aacaatcact ttgctttcgt tgcttccgaa gtcatctgcc
    13681 gtccaggctt gcgccatgac gcgcaattca ccgttaaaat cgccaattgg cagtgtaacc
    13741 gagccttcgc cctgttcatt gagcgttacc ggcagcgcct gctgcgcgac aatattgacg
    13801 tgattgaccg gcggtttacc accacgtttc agctcatcac catcgccacc aaaacgcaga
    13861 gctgccagac gcccctgacc ttcaatgacc tgaccgtaaa tatcgtaaat gtctgcgcca
    13921 tagcgtttct gaccaaagaa cgcctgccac ggatctggcg tgacgtaatc agtaatattc
    13981 agcacaccgc tatcaaccgc agacaccagc acattcacct gttttggcat ctcaccattt
    14041 ttattgctgg ctttaatttt cacggttaat ggctgattgg ggcgcatttt ggtgggggtt
    14101 tccagcgcca gatcgaggcg acggttttca tcaccaagcg gcagatgcaa caccccaacc
    14161 gcgcgttttg gcgtcgcgga gcgagattta tcgccaggac gtaccaccag cgtacttaaa
    14221 tacagatcat gacgattcca ggttttatcg accggaatcg tcagatccag cccttgagcc
    14281 ggaacatcaa tctcttgcca ccacagcggc ccttcactgg actcgaccat cgcataacct
    14341 ttacccgccg ttggcgcggc aatatgcaac ttaatggtat cgccagggcg atagctggct
    14401 ttatccagtt tcagcgtgac acggtcgggt cgcactgcac cacccccgtc gctgttatcc
    14461 tgccagctat aaccagccca gaaacgcaca ctactgaccg cttcattcgg cgctttaact
    14521 tccagacgat aagcgcccca ctctaccggg aaactgacct taccggtttc gtccgctttc
    14581 agatccagag tttgttcatt ttcgatcaga tctttttgat caaactgtga ctgccagcct
    14641 tcatattccg accagttcca gtagtaatcg cggcgttcgc gaatcaggcg cacctgcaag
    14701 cccgacacgg cttttttcac gccttgcgca tcgctataaa cgatgtcaaa agcggcgtta
    14761 ctgccttcat caacaatggg ctgtttcacc gtgctgtcgg tgcgataatc gtaaaccgat
    14821 ttcgaggcga actgcggacg gatccccggc aatgcatcgg caggccagat agcctgctca
    14881 gcacggcgcg tcaccgggcg accgcccgat tccagcaaac taccctggaa aataacctgt
    14941 aatggggaat gcgtttcctt ccactggctt tctgtagaaa cttcgccgcg ccctttatca
    15001 tccagcgtca actgaacttc atccagcgtg cgggaaagat tttcggcagc tatatctccg
    15061 aattcaaaac caggtaaggc tgacacagct tcacgcagtg ggcgcaggaa aagttgccct
    15121 tgcaaaatat taccattagc aggcgcacca tacaggtagt accccaccac ggagaatttc
    15181 acttcatctt tcggcgttag cggggttttc tcaccggtca gattcagcgc catgcgctct
    15241 ggcataaaat cttcgacgtg gaaatcccac atccggtact ggttatcgcc cgtgttagcg
    15301 cgaatatgcc acataccggt tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg
    15361 ccattctccg gctgactaac gacgctcctg agtacctgcc cgtcgggttt aatcacgtcc
    15421 agcttgatgg gttgatcggg caatgcttta ccgtctgcat cacgcagcaa accattgagg
    15481 atgaccgttt cacccgggcg ataaagatcg cgcgggccaa acatgaaaaa ctgtttgcta
    15541 tagcccggcg cgccagcaat gttaaattcc gctaagtcca gcgccgggag ttttaaatcg
    15601 agtagcgttg tctgaccgtc tttacgcgcc agcagtaatg ccgcgttttt atcattttcc
    15661 agctgcacat gcccctgagc atcactggtt gcctgagtca gagtctgccc tttctcattt
    15721 aataaagaga cttctattcc ttgctgggcc gcgccgtttt ccagactttg ggtaaagata
    15781 tccagacgat tgtgataacg gtgagctgaa acgccgatat cacttaacgt aaacagcgtc
    15841 gcgggattac tgtaatcgta acgtccagcc tgattcatca cagccagata cacgcctgcc
    15901 tgctgaagcg gtttgatatc gcccagcggc agcaataatt tttcacgggt gttacgcgca
    15961 ggattgagat caaaccgtcc agtgtagacc agatccgcca tctgcagcag tttgtctgac
    16021 tgccagttcg ccagcgaatt gcggtattcc cattggctaa tgaaagcggg cagtgattct
    16081 ggtttcacac ggaagaagtt aacatcgaca ttgttaacgt tgagcgccat taccggcaac
    16141 ccttcaataa ctttaccagg taacaacgaa ccacggctgg caaaaccgac gctgggttgg
    16201 atatcacggg tagttatagt tttttcgtca tctttactaa aggttgcgtt gttgagtgct
    16261 ttgacctcct tgccaatagt gacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgc
    16321 aactctttaa gattatctga cagctcccag gcaccatcca ctttgccgct tttcttatcg
    16381 acgacatgga taacgcgtga gaaatcctga tccgggtcga gaggaatgga gaacgtcagc
    16441 accagcgtgg ctgcgccatc aagttgaact tcagagagat ccagcaatgt cagcgcctta
    16501 ccggcactct gttgggccag tttttgtcgc tccggcgcgg aaagttttgc tgaactcacg
    16561 ttttctgaag aggccgcttt agtaacttca gaaggcgcat cttttttcac cgctgttggc
    16621 gcgttatcgt tgttgtcgca ccctgccagc gccagcatta gcatgcaggc ggctacgcgt
    16681 aactttttca tttttcatcc ctgccacaat ggcccgttag caacgtcgaa taattattat
    16741 gcgtgagaat ttacgttatg taattcagta tcacgcccat acaatccgaa ttttagtgag
    16801 aattgtggct cagacagcca aatcagcctc ttttgcttgt cgcttttcac taaacatccg
    16861 acaatttagc ctaaccccgg caaaaatgga gatgcctatg tccacgacat ggtttgtagg
    16921 agccgactgg ctcgccgaac atattgatga cccggaaatt cagattatcg atgcccgcat
    16981 ggcgtcgcct ggacaggaag atcgtaacgt tgctcaggag tatatgaatg gacatattcc
    17041 cggtgcagtg ttttttgata tcgaagcgct ttctgatcac acttccccgc ttccgcacat
    17101 gctgccgcgc ccggaaacgt tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa
    17161 gcacctgatt gtctatgacg aaggtaatct tttctcagcc ccacgagcat ggtggatgct
    17221 gcgcaccttt ggtgtagaga aagtgtcgat tctgggtggt ggacttgcag gctggcagcg
    17281 tgatgatctg ctattagaag aaggcgcagt agagctgccg gaaggagagt ttaacgccgc
    17341 gtttaatcct gaagccgtgg tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac
    17401 ggcgcaaatt attgatgccc gcccggctac acgttttaac gcagaagttg atgaacctcg
    17461 cccaggttta cgtcgcggac atattcccgg tgcattgaac gttccgtgga cggaactggt
    17521 gcgcgaaggc gaactaaaaa cgaccgatga actggatgcg atattttttg gtcgcggcgt
    17581 cagctatgac aaaccgatta tcgtcaactg cggctctggt gtaacagcag ccgtggtttt
    17641 gttggcactc gcgacgctgg atgtgccaaa cgtgaaactg tacgacggcg catggagtga
    17701 atggggcgca cgagcagatt taccggttga gccagagaaa taagctttta acaggcaata
    17761 aaaaaccgcc gaatttggcg gttttttatt gctggtctgg ttcgcggcct ttccagcaga
    17821 ttggcttgtg ttgcatgagc aacgcaggtg cttcacagca aagcaatact cgccagtaac
    17881 tctctttttg tcaagcgaaa gagagtaatc attgtttatt tagcgtatta tcgacaccgg
    17941 ccctttccgc cgtctcgcaa acgggcgctg gcttaaggaa aggatgttcc atggccgtaa
    18001 atgcaggtgt ttcacagcgc ttgctatcgc ggcaatatcg ccagtggtgc tgtcgtgatg
    18061 cggtcttcgc atggaccgca caatgaagat acggtgcttt tgtatcgtac ttattgtttc
    18121 tggtgcgctg ttaaccgagg taaataataa ccggagtttc tccggcaaca atttactggt
    18181 ggttaacaaa cttcagagca gcaagtaagc ccgaatgccg ccctttgggg cggcatgttt
    18241 taaattatcc ggttctgttt aaagtcacgc aaaaaaccac cccagcgacg ttcatagaat
    18301 ggcgcaatat gttcggtaat aaagtggcta ataccttttt cccctttttt cacctgacaa
    18361 atatcgatgg gttcatcgcc aggtaatgta tcggtcgcta cacttcccgc cgcctgaata
    18421 atttcttcga tatcaccatc ggcttcaatg ccaataagta aattaggctg tgcctcttcg
    18481 ttctctttaa ttgaacaaat aaaagcacgc ttcaccggct taatggtttt aaataaggtg
    18541 gtaagtgaat caatcatttg cgctggcggc tctgcgacct ccgataatat cagcgattca
    18601 ccgccttcca gtacttcctg actgctaagc ggattccctt cttcaccaat cagcaaactg
    18661 atttcacgcg gcataaattc tttaccggtt ggcagtttgg cattaaggaa gagcgtttcg
    18721 ccaagggtca tctcaaacaa tgtgcgaaca ggcattacga caaatgcctg ttcgccttca
    18781 accgcctgtt gaagtgcttc taacgaggtg aaaaaaggaa tgacgctggt gccgtcttct
    18841 ttttcccagt gctgtaaatc aagtgcgcta tcttcaacca cagcctcgcc ctgtgccgcc
    18901 gtaccaggca cccagacggt ggattccagt agagtacgga aaaaggccgg gcggtgcgcc
    18961 ggttcggttg ctgctttttc cagcaggtct tcaagttcgt tttttgtttc ggacataaga
    19021 accataattc attcaacgtt cggggcgtaa gtgcctgatg cgctacgctt atcaggccta
    19081 caagggaacc gcaatatatt gaatttgcaa tactttgtag gccggataag gcatttacgc
    19141 cgcatccggc atattagttt acgccgttaa cagattagca atcgtgcgca caccaagtcc
    19201 cgtagcaccc gcagaccact gttcaaccgg cgctttacgg taagtcgccg agcagtcgat
    19261 atgcagccaa ccttgctgat agttctcaac aaagtgcgac aggaagcccg ccgctgtgct
    19321 cgcgcctgcc ggatacgccg cgcttccggt gttgttcagt tcggcaaagt tagacggcaa
    19381 ttgattgcgg tggaactccg ccagcggcag acgccagaac ggttcattct cctgcgcggc
    19441 actcgccaac aagcgaccgg caagcgcatc gtcaaaactg aacagcgcgt gataatcatt
    19501 acccagcgcg gttttcgccg ccccggtgag ggtcgccgca tcaatgatca gttccggttt
    19561 ctgcgcactg gcatcaatca ggccatcggc cagcaccaga cgcccttcgg catcggtgtt
    19621 catcacttca acttttttac cgttgcgata ggtaataata tcgcccagtt tgaacgcgtt
    19681 gccgctgatc aggttatccg cacaacagag gaacagcttc acgcgcttgt tcagtccgcg
    19741 cgtaatggca aatgccagcg ccccggtaac cgttgccgca ccgcccatgt ccgacttcat
    19801 cgagtccata aacgcagtct gtttgatgct gtagccgccg gaatcaaaag tgatgccttt
    19861 accaaccagg cacgcgtaca ctggcgcttc tttatcgcca gttgggttgt aatccagcgc
    19921 cagcaatacc ggagaacgtt ctgaaccgcg cccgaccgtg tgcagcccca tataaccttg
    19981 ctcacgcaga tcttcgcctt tggtgatccg gtaagtcaca cgatcccctg cgacgttgct
    20041 gatcagatca acagcacgct gtgccagttg cgatggcccc agctcttctg ccggtgcgtt
    20101 gatggtgtca cgcacccagt cgatgatcat caggcggtta tccagttcct gacgctgggc
    20161 atcgtccaga tccggccact ccactttacg tatgcctttc ggggctttgt aaccttgcca
    20221 gaatgcccag cagcgatccg catcccagcc ttcaccgctt aactgaacat gcttgatgcc
    20281 cagaccgtca atcttgcgcg ccgcacgctg gatcaacccc agatcgtctg ccccgttcaa
    20341 atgcagggta atgccatcat tattaatgct gtaagtcgct ttttctcccc aacgtgcgtc
    20401 ggcaggttgg ggagagaggg taatcttcat cgcttctgtc attttagtta tccttattcg
    20461 tttaacagca aaacgggcta cccccaggca gcccgttcat tcactcggct tcgtccagcc
    20521 agaccaacaa aatcgcttcg aggattttct cgttcgatgc ctgcgggtcg tcgtcgaaat
    20581 cttccagagc gcaaatccac tgatgcatat cggtgaatcg aaccgttttc ggatcaatat
    20641 cgggatacgc atcgtacagt gcttcgccaa tttcgcggct atcggtccac ttaagtccca
    20701 tactaacctc tgttaatgct cacgcgcatg gttgatagtg taacgcggga tttcaaccac
    20761 taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg ctttccggct ccagtcccca
    20821 ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc ggcagcgagt caaaaccttc
    20881 acgaacgatg cagtggcagg tggtgcaagc acaggatttt tcacaggcgt gttcaatctc
    20941 gataccgtta cgcagcgcaa cgtcgagaat ggtttcaccg ctattcgctt ccagaacagc
    21001 gccatcaggg cagagatcct gatgaggcaa aataacaatc tttggcatat taaacctcgt
    21061 ccacggaatg gcctttcagc gcacgacgaa ccgactggtc catgcggcga gcggcgaaat
    21121 cctgggtttg tttgtctacg tttttaatcg cttgttcgat ggcgtcaaca tcatcgccct
    21181 gcgccacttc actcaggtga gcagcagcat cgtcgatagc ctgacgttct gcggcgctta
    21241 acagcgcggc atcagcagcc agcgcaccgt gcagactttc cagcacacgc gccgcttcta
    21301 ctttttgttc tgccagcatt cgggctttta cgtcctgctc ggcatagctc attgagtctt
    21361 tgatcatcga ggcgatttcg ctatcggtca ggccataaga cggtttgacc tgaatagacg
    21421 cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa aagaccgtcg gcatcgacct
    21481 ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc cggaatacct cgcagcgcaa
    21541 aacgcgccag cgagcggcag tcctgcacca gttcgcgctc accttgcatc acatggatag
    21601 acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg ggcgcgggcc accggaatcg
    21661 tggtattacg cgggatcact ttctccacca ggccgcccat cgtttcgagg cccagcgaca
    21721 gcgggatcac atccagcaac agcatttcgc tgtccggctt gttacccacc agaatatccg
    21781 cctgaatcgc cgcgccaata gcgacgactt tatccgggtc gatagaagtc agcggtggac
    21841 gaccgaaaaa ttcgcctact cgttcacgca ccagcggcac gcgagtagaa ccgcccacca
    21901 tcaccacttc cagcacttca tcagcttcta cgcccgcatc tttcagcgcg cgacgacaag
    21961 ccagtaaggt tcgttttacc agtggcgcga tcagttcatt gaattgttca cggctgattt
    22021 cgccctgcca gcccgcaacg ttaacggtca cggagaccgc atcgctcagc gcgattttgg
    22081 ccgcaatggc agcatcgagc agttcacgct gaacgcggtt atcgctacga tcaggaatgc
    22141 ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa atcatcgccg ccgagcgcgg
    22201 aatcaccgcc ggtcgccagc acttcaaaca cgccgcgact taagcgcaga atggaaatat
    22261 caaacgtccc gccgccgagg tcataaacag cgatcacgcc ttcctggccg gaatccagcc
    22321 cgtaggcaat agccgcagcg gtcggttcat taagtaagcg cagaacatgc agccccgcca
    22381 gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc aaagtacgct ggaacggtga
    22441 taacgacacc atccagctcg cctgccaggg cttcagttgc ccgcgccgcc agtgctttga
    22501 ggatgtccgc agaaacgcgc accgggttca gcagccccgc cgccgtttca atcatcggca
    22561 ggccattttc gctggcctgg aattgataag gcagatgcgg atagcgttgc tggatatcag
    22621 ccagcgagcg ccccatcagg cgtttaacag aactaatggt gttggcggtg tcgagcgctg
    22681 cgttagtacg cgcgtcataa cccaccgaat gcccttgctg ttgatagtga acaacagatg
    22741 gcagcaggtg acggccttca tggtcagcta acgtttcggc ctgaccgctg cgcactgttg
    22801 ccaccagcga gttggttgtg cccaggtcaa taccggccgc caggcgacgc tgatgcggcg
    22861 cagcactcaa accaggttca ctaatttgta ataaggccat gtttagcttc cagaaattaa
    22921 aaatcgagca gtttttcttc gagttgttcg gcactgctcc gcagtttatc gagaaaacgc
    22981 agcttacgca cggtatccgc cgccgcgtcc cacgtctcgt tgtctaactg ttcaaccatc
    23041 aactgatggc gggtatcaaa catctttttc acacgtttga taaagctttc cagccgcgct
    23101 tcatctttcg cctgttcgat ctcgtccagt tcttcgcgca gttccagctg ttccatcagg
    23161 aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat caaaaccgtg caaagaaagc
    23221 aaatattccg cgcgcattaa cggatgacgc agcgtttgcc aggcctggtt aatggtcgcg
    23281 gattgctgta cggcggcgag ttgttccgcc tggcttccgc tggcgaattt atcaggatga
    23341 tactgacgtt gtagatcctg aaaacgcagg ctcagcgcct gggtatcgag ttgataacgg
    23401 gcaggcaagc caaagagggt gaagtaatcc ataacattct cagggctgcg atccgccgcg
    23461 ataacggcga atcaggtaaa acaaacccca cgcgcaggcg accacggtgg ggttatcggt
    23521 atgcgcatca aacgtggaag ctttcgccgc aaccacactc atctttgacg ttcgggttgg
    23581 tgaatttaaa cccttcgtta aggccttctt tgacgaagtc cagctgcgta ccgtccagaa
    23641 attgcaggct tttgccatcg accacgactt tcacgccttt gtcttcaaac acgatgtctt
    23701 ccggcgtcgg ttcgtcaaca aattccagta cataagccat acctgaacac ccggaggttc
    23761 tcacgcccag acgcaggcca aaccctttac cgcggttagc cagaaaggta tttactcgcg
    23821 ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc tcaactctta ttttgcttca
    23881 cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt cttctgccag aatagaacag
    23941 tgaattttca ccggcggcag ttcaagttct tcagcaatat cggtgttttt tatcgcctgc
    24001 gcttcgtcga gagacttccc tttcacccat tcggtgacca gggagctgga agcgatagcg
    24061 gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga taccttcatc gttgacttta
    24121 atctgcaact tcatcacgtc gccacaggcc ggtgcaccca ccatgccgct gccgacgttc
    24181 tcgtcgttgt tgtcaaagga acccacgtta cgcggattct cgtaatggtc gataactttt
    24241 tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg agcccattcg
    24301 atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag gtcacgcaga
    24361 cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc agtagtaaaa
    24421 cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc cagcgcgcgc
    24481 agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga aaccgcgagg
    24541 tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac gttgagaatg
    24601 ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc tttgatgccg
    24661 ttccacagac ggttacgcag gccgcgcaga cgttccatct cggtcgccat ctcttctttt
    24721 gcgatgcgat aggcttcgcc cataccgacg atctggtgaa caggcagagt gccggaacgc
    24781 ataccgcgct cgtgaccgcc gccatgcatt tgtgcttcga tacgtacgcg cggtttacga
    24841 cgcacataca gcgcaccaat acctttcggg ccatagattt tgtgaccgga gaaagacatc
    24901 aggtcaactt tcaactggct cagatcgatc ggcagtttgc ccacgctctg ggttgcatca
    24961 acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat atcctgcacc
    25021 acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcatc acgcatcgct
    25081 gcttccagtt ctttcaggtc gataatgccg ttacgctgcg gtgccaggta agtgacttca
    25141 aaaccttcgc gctccagctg acggcaggta tccagtaccg ctttatgttc ggttttactg
    25201 gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat cgccaggttg
    25261 tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg
    25321 gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa acggtgagaa
    25381 cgggaggccg ggttaccaaa ggttccgtcc atcgtcataa actgcatcat tttctcggca
    25441 acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa tttcattgct
    25501 ctataaactc cgtacatcac tcaatgcaag gaaccaggct accggctgga tgtacgaccg
    25561 tgtttacgga gtgtttagca ctccggcctg attctgaatt ctttttatta agcgcgtaac
    25621 ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacggcca
    25681 gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt attgagaaaa
    25741 ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcactt atcgccgccc
    25801 tgacaaccgc ctttaccctg acaacgggtg gcatctacag attcatcaac ggcgctaatc
    25861 acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca
    25921 cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa
    25981 agggaaattc cctgacgttc agaaatatca gccaacggta ccgggcccgc ttcagagttg
    26041 agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct
    26101 tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca
    26161 tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta
    26221 gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtcggat
    26281 gcggtgtgaa caccttatcc gaccttggat tccgtgcact tacaggcctg ataagacgcg
    26341 ttaagcatcg catcaggcaa ccactgttat tcggccttgt tacctttatt ctgctgctca
    26401 atagaagcca gaatcccgcg caggatattc aattcctggc tttccgggcg tgcacgggta
    26461 aacagacggc gcaatttatt catcacctgc cccggatggt tttcacggat aaaaccggtt
    26521 gccagcagcg tttgctccag atgaccataa aaacgctcca gatcatcgac tagcggatac
    26581 ggcgtctctt catgctcgac ctgctcgcca ttttcctgag ttgccagcca ggccatgcgc
    26641 acttcatagg caataacctg aaccgccatc gccaggttca gcgaactgta ttccgggtta
    26701 gccgcaatcg cgacatgata atggcatttc tgcaactctt cattggtcaa gccgacgcgc
    26761 tcgcgaccaa acaccagcgc caccggggta tttgccgctt cagcgacgct tttcagaccg
    26821 cattcgcgcg ggtcgagcat cggccacggc agcgtgcggg agcgagcgct ggtgcccacc
    26881 accagactac aaccggctaa cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc
    26941 acatcgctgg cccctgctgc cagggcaatc gcctgggagt caggtttcac tagtggatta
    27001 accagccaca gattggttaa tcccattgtt ttcatggcac gggcaacaga acccatattg
    27061 ccggtgtgtg acgtctccac cagcacaatt cgaatatttt gcagcattgt ctttcttcat
    27121 ctaaagatta ttcacgcatc ttatcataaa acgaagacag atgccgatct cgctgctata
    27181 ctctgcgccg ttttcccgtt ctttaacatc cagtgagaga gaccgatgca tccgatgctg
    27241 aacatcgccg tgcgcgcagc gcgcaaggcg ggtaatttaa ttgccaaaaa ctatgaaacc
    27301 ccggacgctg tagaagcgag ccagaaaggc agtaacgatt tcgtgaccaa cgtagataaa
    27361 gctgccgaag cggtgattat cgacacgatt cgtaaatctt acccacagca caccatcatc
    27421 accgaagaaa gcggtgaact tgaaggtact gatcaggatg ttcaatgggt tatcgatcca
    27481 ctggatggca ctaccaactt tatcaaacgt ctgccgcact tcgcggtatc tatcgccgtt
    27541 cgtatcaaag gccgcaccga agttgctgtg gtatacgatc ctatgcgtaa cgaactgttc
    27601 accgccactc gcggtcaggg cgcacagctg aacggctacc gtctgcgcgg cagcaccgct
    27661 cgcgatctcg acggtactat tctggcgacc ggcttcccgt tcaaagcaaa acagtacgcc
    27721 actacctaca tcaacatcgt cggcaaactg ttcaacgaat gtgcagactt ccgtcgtacc
    27781 ggttctgcgg cgctggatct ggcttacgtc gctgcgggtc gcgttgacgg tttctttgaa
    27841 atcggtctgc gcccgtggga tttcgcggca ggcgagctgc tggttcgtga agcgggcggc
    27901 atcgtcagcg acttcaccgg tggtcataac tatatgctga ccggtaacat cgttgctggt
    27961 aacccgcgcg ttgttaaagc catgctggcg aacatgcgtg acgagttaag cgatgctctg
    28021 aagcgttaat gactcaggcg ggtgatatca ctcacccgcc ctcgcctttc aggcgctatt
    28081 ccgaaatact tcctcaccgc tttactttct ttcgtcactc tcccaccatc tttccccgat
    28141 taatggatga taaagaagtc gcaaggaaag gttatggcgc tgccagtgaa caaacgcgtt
    28201 ctcaaaattc tgtttattct ctttgttgtt gccttctgcg tttatttagt gccgcgcgtc
    28261 gccatcaact tcttctatta tcctgacgat aaaatatacg gtcccgaccc ctggtcggcg
    28321 gaatccgtcg aatttacggc taaggacggt actcgtctgc aaggctggtt tattccttct
    28381 tcgacgggcc ctgctgacaa cgccatcgca accatcattc atgctcacgg caatgccgga
    28441 aatatgtccg cccactggcc gctggtcagt tggttacccg agcgtaattt caacgttttt
    28501 atgtttgatt atcgcgggtt tggtaaatca aaaggcacgc cgtcccaggc cggattgctg
    28561 gacgatacgc aaagtgccat caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg
    28621 ctggtgctgt tcgggcagag tattggcggg gcgaatattc tggatgttat tggtcagggt
    28681 gatcgtgaag gcatacgtgc ggtgatcctc gactccacct ttgcctctta tgcgaccatc
    28741 gccaaccaga tgctccccgg cagtggctac ttacttgatg agagttacag cggtgaaaat
    28801 tacatcgcca gcgtcagccc gatcccgctt ttactcattc acggtaaagc tgatcacgtt
    28861 atcccatggc agcacagtga aaagttgtat agcctggcaa aagaaccaaa acggttgatc
    28921 ctaatcccgg atggcgaaca cattgatgct ttttccgatc gtcacggcga tgtttatcgc
    28981 gaacagatgg tggattttat ccttagcgca ttgaatccgc agaactaacc catgatcgct
    29041 agcacgataa tcattcacaa aaccacctta agacatgcta atccactggt cagaacagtt
    29101 taagatgaga aaaattctgt gacgcttgcc aacatttctg atgattagca ttcccttcgc
    29161 catttccctg agcaaacttt agctattctt atcaattatg cttatgggag atctacagat
    29221 gatgcctacg cttgctccac catctgtcct ttcggctccc cagcgccgct gccagatctt
    29281 gctgacgctc tttcagccgg ggttgaccgc caacacggca actttcagcg agcttaatgg
    29341 tgtggatgat gatattgcca gtcttgatat cagcgaaaca ggacgggaga tcctgcgcta
    29401 tcatcaactc acactgacta ctggttatga cggcagctac cgggttgaag gtacagtgct
    29461 taaccaacat ttgtgtttat ttcactggct acgacgtggt ttccgtctgt gtccgtcatt
    29521 tattaccagc cgattcaccc ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg
    29581 taacttttac gacgatacca atctacaagc gttagtgaat ctctgctctc gacggctgca
    29641 aaaacgtttt gaaacgcgcg atattcattt cctgtgtctg tatttgcaat attgtttgtt
    29701 gcaacatcac gctggaatta cgcctcagtt taatccgctc caacgtcgct gggcggaatc
    29761 ctgccttgaa tttcaggtag cgcaggaaat tggacgccac tggcagcgtc gggcactcca
    29821 gcctgtaccg cctgatgagc cactgtttat ggctctactg ttttccatgt tgcgggttcc
    29881 cgatccactg cgggatgcgc atcagcggga cagacaactg cgtcagtcta tcaaacgtct
    29941 ggtaaaccat tttcgtgagc tgggaaatgt tcgtttttat gatgaacagg ggttatgcga
    30001 tcagctttat acccacctcg cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga
    30061 taacaccctg ccggaagagt tcgcccgcct gtatccacgc ctggtgcgca ccacccgcgc
    30121 ggcgctggcc ggatttgaaa gtgaatacgg cgtccatctt tctgatgagg aaagcggtct
    30181 ggtagcggtg atttttggtg cctggctaat gcaggaaaac gacctgcatg agaaacagat
    30241 tattctactg accgggaatg atagcgagcg agaagcgcag attgagcagc agctacgcga
    30301 actaacgtta ctgccgctca acattaaaca tatgtcggta aaggcatttt tgcagacagg
    30361 cgctccgcgc ggcgcggcac tgattattgc gccttatacc atgccgttac cgctcttttc
    30421 accaccgctg atctatacgg acctgacatt gacaacacat caacaggagc agatccgcaa
    30481 aatgctcgaa tcagcatgag ggaacaactt tcgggcgcaa aaacattgcc ggaagcgcca
    30541 ccagcgccat cacccagaac acgccgtggc cgagatattg atacaggaaa ccggcgaaaa
    30601 cggtcatgat ggcgatactg ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga
    30661 tgacttcgct accctggcga gcagcaatat agcgcatggc agccaggtgg cagaccgtga
    30721 aggtaccgca atgcagaatt tgcaccacta tcaaccacgg caacgccgtc gttgctccca
    30781 taatgcccca gcgcactacg ccgcaaatcg ccgagatcaa cagcatatcg cgtgcactac
    30841 aacggcggaa aagtttatta ctcagcgcaa agataatgac ttccgccacc acgcccagcg
    30901 accacaaata ccccaccgcc gaggccgagt agccagctgc ctgccagtaa atggcgctaa
    30961 aaccgtaata ggccgcatgt gccccctgca ataaacaaac gcaggccaga aagcgccagt
    31021 tctggcgaac caacgccaac cacgcagacc agccggtgct ctcctgctgg cggcttgccc
    31081 cttgtggctg aatcgtcgga cggatgagaa agccgagcag catggatgcc acgcccaacg
    31141 tcaacagcgc gaggatcacc cgataatcaa acatactgac cagtttgccc gtcagcgccg
    31201 agccaatgac aaacgccacc gagccccaca gtctcacttt gccgtaatca agcgggaact
    31261 gcttttgcca cgtattcgcc agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt
    31321 taaagccaat catcaccagc atcagccacg ctacgtgcgc ccccgcccag aaggcgacag
    31381 caaagagaag tgtcagcagt gccagcacgc gcaaggcgga aatcaggcgg gaaggatcgc
    31441 tgacgcgggg cgcgatgagc aaactcccga ggaaacgggc aaccagaccc gcccccaata
    31501 acaggccgat ggtttctggc gttaaaccaa tccctttaag ccagacgctc cagaaaggta
    31561 gaaaaatgcc gtaactaaaa aagtatgtga aatagccgag cgccaaccag cgcgtggatt
    31621 gcaaaaccat gagtccctcc cgtcaggagg cgttagtctg gggttaatcg aatgttggtg
    31681 caagttgaaa aataagaata ttcgagcctg cttcctgacg gttatcctgc atatcaggaa
    31741 atgacaaaaa agagcggccc acaacacaac atgtaaaaaa gaacgatgaa acaggtgaaa
    31801 atcgcaattt attgaactga caaactcatg tgagacggat aaggcgtgca tgtcgcatcc
    31861 ggcaggaaac aataagcaca ttgtcagcca tctgcgttat gccgttatac cagaaacgaa
    31921 tatctcaacg cctgggcgtg gcgaggtacc agagtgtgta ttacgtttaa atcacatcat
    31981 cttgcaaagg gattggttat gaactcacta cgttattttg attttggcac tgcccgcccc
    32041 gttttattat taattgcccg tatcgccgtg gtcttaattt tcattatttt tggttttccc
    32101 aaaatgatgg gctttgacgg tacggtccaa tatatggcct cgctgggcgc accaatgccg
    32161 atgctggcag cgattattgc ggtagttatg gaagtgcccg ccgcgatatt aatcgtgctt
    32221 ggctttttca cccgtccgct ggcggtgctg tttattttct acacgctggg tacggcggtg
    32281 attggccacc attactggga tatgaccggc gatgcggttg ggccaaatat gattaatttc
    32341 tggaagaata tcagcattgc tggcgcattc ctgctgctgg caattaccgg gccgggggca
    32401 atttctctcg atcggcgtta ggaaagatgc cggatgcggc gtaaacgcct tatccggcct
    32461 accgctccaa caccgattgt aggcctgata agacgcagca agcgtcgcat caggcatcgt
    32521 gcaccgagtg ctacaaaatc gaaattaccc cgtaggcctg tgcatccggc attttataaa
    32581 ttacagcaac gcaacccgca acgacatctc tgaaattgtt gattcccctg gtgcaagcgc
    32641 aatgaggtca ccaccttccg gacgatgatg gtcatccggc gcatgactca tcggttccag
    32701 acagaagaaa tcaaacgcat atcctttatc aaacgcaggg tctgaaacaa agatgaaata
    32761 acacggtgca ggtggcgtcg tttccatgat gatggcataa ccctcctgcg gctgctcgat
    32821 gcgggcctga ccattccatc cggcaaaacc attgtttacc cactggtgcg gcaacggcgt
    32881 gagctggtta aaatccagtt cctgcggtag ctgctcgcaa aactcacccg ccagccactg
    32941 ctctcgctcc agccagtaac cgctcgcctg tgcctgaacc cgcgtttgcg gcgacaacgg
    33001 aaaataagga tgccagccgg taccaaatgg cagcgtctct gccccttgat tggtgacagc
    33061 gagcgtcacc gtcagcgtat ccgccgttaa atgaaatgcc tggcttaccc gatagtgata
    33121 aacaccgctg agatgttcat acaccagaca caagctatca tcgctgcgcg agacacattg
    33181 ccactcgccc aaccagccat cgccgtgtag atagtgcgca tcccactcaa cattcggttg
    33241 cagttgatac tcacgcccct gccagacaaa acggttgccg ctcacccggt ttgcaaatgg
    33301 taccagcgga aaacaagagg catcggttgc cacaccgctt tttttaccag gacgtagcag
    33361 cggcgtcgta tcgcgccaga agccttcgat tacgccgccc tgatcagaaa cgtccagctt
    33421 tagcggcccg tgagataggg tatagatggt catccgcaga ctccttaatc cgggaaatta
    33481 atcacaactt tcccgcattt gccgcttgcc atcagtgcat aagcatctcc cgcctgttcc
    33541 agcgagaagc ggtgggtaat ggcgttacgc ggccacagtt tccagtccgt taaatcgtgg
    33601 gcgcattttt ccatatggaa cagactggtc acccaggagc cgataatccg ccgttgatgg
    33661 tgcatcaggt cggcgctaac ctcgaactcg acttttccgg tttcaccaat gtaaaccacg
    33721 cgtccccagt cagcggtgga ttgcagtgcc agtaagcgac ctgcggcatt accggaacaa
    33781 tcaagcgcaa catccgcgcc gccgtgagtg agttcggcga ttatctgcgg cataccttcg
    33841 gtggtcgcta aatagccgtg atccatcact cctaactgtt tcgccatcgc cagacgttcc
    33901 ggcaacatat caacgccgat gatccgtttt gcaccgcgac ctttcgccag catcatcgcc
    33961 atcatgccga ccggccccag accaaccacc accacgttat cgctaccgga aacctcgccg
    34021 cgcaaaatcc cttcatacgc cgtaccaaca ccgcaactga taaacgcgcc atcttcgtaa
    34081 ctcagcgcgt ctggcaggag gatcagatct ttttcttccg ccagtaggta ttcggcatga
    34141 ccgccgtcac gctgccagcc gtaagccgct tttccttcgc cagtacaaga aataggaaaa
    34201 ccgcgacggc agttcgggca aaaaccacag ccagaaatgt gatacaccag cacgcggtcg
    34261 ccctctttaa aatggcgaca gccttgcccc atcgccacaa tctgcccgca cggttcatga
    34321 ccgttgataa atccctggta taacggttta tcgggtgccg ccgccgtagc gcggtgctgg
    34381 tggtagatat agtggacatc gcttccgcaa atcccggagg atttcatttt gatcagtacc
    34441 tggttaatcc ccggcgtcgg caccgcaact tcccgcagat cgacggtcga atttcctggt
    34501 aaataagctg ccagcatcgt tttcataaat ccctcattaa caatacgatt aattttcatc
    34561 cctgccgcac ccgcgccagg ggcgttaagg tttagcgttt cgctttactg ctacgctggg
    34621 tcagcaagat attcgccagc accgccacca cgatgatgac gccgcgcacc acctgctgga
    34681 aaaaggagtt aatcccgagc agcaccagac cgttaccgat tagcgtaata accagtacgc
    34741 caagcagcgt accgaacaac gagccgcgac cgccggaaag cgccgtaccg ccgaccacga
    34801 ccgcggcgat gacgtcaaac tccagaccgt ttgcggcacc tgcgttaccg gaaccgaggc
    34861 gggccgccaa caaaatgccg gtcaccgccg ctaataatcc cgaaagggta aaaataagaa
    34921 tgcgtacccg acgaacgtta ataccgcaca actgcgctgc agtggcatta ccgccaacag
    34981 caaaaaccga gcgcccgaag gcagttttgc ggctaatgaa cacaaacagc gcaaataaca
    35041 ccatcatgat cagcgcggat accggcacac cgagaaattg tccgcccagc caatccagca
    35101 cttcgttttc gttaattggc actggcaagg cgttcgtcat aaacagcccc attccgcgca
    35161 gggcgctcca taaacccagc gtggcaacaa aacttggcac gctaaacacg ccgcgcagca
    35221 ccccggcgag cgttcccatc agcgcaccta acagcaacac cagcagacaa gcaatcgcca
    35281 gcggaacgtc aaattgcagc aaaaacgcca ggcacactga aacaaaagcc accatcggcc
    35341 caacgctgac atcaatttca ccggagataa taatcagcgt catcgcccag gcggcgatcc
    35401 caatggtggc ggcatcgcgc agcacgttca tctggttatt caatgagata aagccaggcg
    35461 cattcaggga gaagaccaga taaagaatgg caatcaccac cagcaaaccg atctcattaa
    35521 tatggcgact gacaaattgt ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg
    35581 aagcagacat cttcaggttc ctcggtaaaa ttcatcagtg cacagacaga atggcggaca
    35641 tcagctcatc cacattgacc ggagagtgaa actcctgcga gaacgtgccg tgctgtaata
    35701 acaggatgcg gtcacacacc agcggcaact cctccacttc actggagata aacaccacgc
    35761 tttttccttc ggcagccagc tcgcggacaa tacggtaaat ctgctgtttg gcttcgatat
    35821 cgacgccgcg cgttggctcg tcgagtaaca aaatctggct ggcggcatag acccaacgac
    35881 cgatcaccac tttttgctga ttgccgccag aaagcgtgcc gatgggcgtt tcgctactgg
    35941 cggccttgac cgtcatccgc tgcatcacct cttcggtcag gcggcggatg gtggaccatt
    36001 gcagtacacc gttggcgctg attttttgcc gattggtcag cactgtattt tcgtcaacgc
    36061 ccaaccaggg aatgatcccc gcttctttgc ggttttctgg cgtatagcca atgccgcgtt
    36121 tcagcatgtc gccgtaatcg gggcgcgtga ttttctcgcc gttgataaca atttcgccct
    36181 gttcatacgt ctccagccca acaatcgcct tcagcaattc actgcgccct gcccccagca
    36241 gaccagcaat gccgagcact tcgccacgac gtagtgtaaa actgatatcc tccagcttgg
    36301 gcttatggcg taacgcacgg acttccagca cggcctgatc cacaatttcc tgaggggcaa
    36361 ccggcgcaat atcaacgtga tcgcgcccga gcattagcga cacaatatga tgcgtagagg
    36421 tgtttttgag catcacatcg cccgccacct gaccatcgcg cataacggtg gcacaggagg
    36481 caatgcggcg aatttcttcc atccggtggc tgacataaat caccgccacg cccagtgccg
    36541 acatcttttt caccgcgctg atcaccagtt caacttccgc actggcaagc gagctggtgg
    36601 gttcatcaag aatgaccacg cgcggctcgc ccttcatcac ccgcgcaatt tccaccagtt
    36661 gcttttgcgc cgggcttagt gttgaaacaa gttgttcagg actaacgtca acgcccaacg
    36721 cctgcaagca acgttgggcg tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc
    36781 ggggccactg accgaggcag aggttttccg ccactgtcag cccttccacc agacttaact
    36841 cctgataaac cgcacgaacc cccagttcag cggcacggcg agtcagcgta gcttcgtcac
    36901 cttccagtcg cgtctcgcca atccagatat caccgctatc cggacgttcg ctaccggtaa
    36961 gcattcgaat gagggtcgat tttcccgcac cgtttttgcc taacagcgcg cgaacttcgc
    37021 ctttattgag cgtgaagtta acgttatcca gcgcaacgac gccggggtaa cgcttatttc
    37081 ctgccaccac ttttgctacc gggactgcct ctgttgccgt gaacataggg acctctgcga
    37141 atcagcgatt agggcagacc atcaacgtgc gttgccagcc actgtttacc gtcttccgtt
    37201 ttggtataga gatcgattgg cacctgaatc actttttcac cgtcggcttg tttattgata
    37261 accttcaatg tttgcgtaaa aacagcattg cccattttct taccggaaat atccaccacc
    37321 gctttcagca cctggttgtt ttccagctcc tgagcaattt cagtggtcat atcagaaccg
    37381 aaaacagcaa tttttccggc ctgattttga ttacgtaccg cttttaccgc gccgagtgtc
    37441 gctccgcccg attcccccat aatggcgttg agatccggcg tggagataat cagtttttca
    37501 ccgacagaaa tcgctttatc taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg
    37561 ggaacgcggg cttttaatac ttcttcaaac cctttacgtc gttgcacaca aacttcaaag
    37621 gcttcgcaat tgatgacggc aattttcggc tggtcaattt tgttggcaat aaaataatcg
    37681 gcggcagcgt tacccagttt tttaccaaat tccagcgggt caccaaccag atatgccgag
    37741 acatatttat cgacaccctt ttgattaata caggtgttgt agcaaatcac tggaatgccc
    37801 gcctcactgg cgcgacgaac ggtgcggctg ctgccatttt cagaaacagc tgacaaaata
    37861 atggcatcga cattacgcgc cacgagggta tcaacaaagg tactttcttt cgaaatatca
    37921 ccctgggcgt tagtttcaat taactgtacc tgtactgaag aatcttttgc cgcatcctga
    37981 acgccctgac gcactccggc gtaatatccc tgggtatcaa ggtatattgc gccaatagtc
    38041 atttctttat cagaagccct ggcaaatagt gcgctaccca atagcgtagc cattaataat
    38101 aaattacggg ttcttgtcat ttttttaggc atagtgcctc ctgtagggtt tttatttaca
    38161 acggcttatt ttaattattt gagcgtgagc ggcagcgcgt gccgctcaaa tattacagtt
    38221 aatagggttt agtgaacgta gctaaaaata aaaggcatca cggtggtgaa taatgccgct
    38281 ttcggccagg ctggattaat cgccatcaat tcagcgcgag cagattcata gtgcgcaacc
    38341 tcccctaatc ccgcggccgc cagcatcgcc accatcaggc atttttcttt atgctgctgt
    38401 tgtaaatcgc catacagcga caacaagtca ggctgcgaga cggcaaagaa atccgcttcg
    38461 atactggttt tcgccatctc ttgtgcccac tgtttcattt cactaaacag ttgctgtgcg
    38521 gtgtgttgtt cgcccagcag tcgcagcgcc attccttgcc agaagagata atcaaccggc
    38581 tgatcgttgt aataactgtg gatgttaatg gtgcgatcgc cggtcgccgc cagacgtaaa
    38641 caacgcgtcg cttcagtttc atcgccctgc gcgttggcgc atactgcctg ccagaaccag
    38701 atgtcgttgt cagtttgccc cggtaaacgg ccttcgctta aattctccgg ataatgcagc
    38761 gcggcatgaa gcagttcgct ggcctgctgc ggctctctgg catcaagatg ctgccaggcg
    38821 cgtaataact ggttgaggat aaactggctg gtgaccttcc cttccccacc ttcccacggg
    38881 tggaatttgc gcgtggcgag aatgtccgcc gctttgtctg cctgaccagt gagatgccac
    38941 aaattgagca gttctgcggt catgtcgtcg cgtttcagcg caatttccag attattttcc
    39001 agacgcgcca gtcgtttctc cggtgtgaca ccgcttaact tatccagcaa atcccgttcg
    39061 aaaagcagac gtgcatcctg cggcgcaagc tgataagcat tatcaagata acgcgcggcc
    39121 agctcataat cgtgctgctt attccacgca tggatcgcca acccgcgcca gccgtcggca
    39181 aactccggcg acatctctac gcaacgctgc cataaggcaa tggctttgcc gtagctacgc
    39241 ttgttgtagt agaagcaggc cagtaaatgc cgggcaaacc agcactcttc gatactctcc
    39301 agcgccgcca cttcttccag cgtattcggg aaacggacaa actgcgggaa ggcatcaata
    39361 gcttttacga ccagttctcc gcgttcagct tttggtagca aactggcttg caggtaaagc
    39421 ggtagcgtgc gctgacagtc cagtgcgttc agcatctctg ccgccagggc gggcatgccc
    39481 cagttaatca gttgcccggc ggtcatcagg gcgttaacgt cgcgtccctg acaaagacct
    39541 cgccactgcg cgagggctga ttcgctacga ccatcgaacc agttcagcca ccacagagtg
    39601 gcgttcagcg gataatcacg cagcagtttc tcgcgctgca aacgcgcgtt gtcctgacga
    39661 ccacttaaca ccagtagcag gttatgcagg caaagcactt cctgattggt tgggcaggcg
    39721 cgaagacttt gttggcaaaa atccagacca gtgtcgaagt taccattacg cgccgccagt
    39781 cgtgccagac cataatagcc accggcttta ctgttgccgc tccagaccgc acgccagaaa
    39841 tcctcttcgg cttgttgata ttgcccctga cgttcgtaag cactggcgcg aatcaaactc
    39901 gcctgtccgc actgcggatt tttgttcagc gcatgtgcgc gtttcagtgc ctgactggca
    39961 tataccaccg cttgcgggaa atctgcacgg ttatattcca gcattgccag cgccagatta
    40021 cagcgataat ccagcggatc cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg
    40081 ctcgcgtgat gatattgctc cagatgttga ccgataaacc aggtttcatc tgtactggta
    40141 atgtcttgtg ctgccagtgg cgctttggcg acgtccggca acggcaacac ttgcggctga
    40201 tgttcctgat aactcagtat gatattgccg tcggcatcgg agagctcaat ggtcagcctt
    40261 tcaggattga taccgtgcaa cacgccctgg atggcggtcg ctggcatcag tgccacggca
    40321 tcatcaagta acgcgttgca tttgccgatt tcgcggatcg ccaggcggta tccgttcaac
    40381 ggagagatgg catacaaccc ccactcaatc ccccgatcac tacgctggag ttttatcacc
    40441 gcatcgcggg aggcgttttg caccatgccc aaagaatggt aaggcaggaa atactgctca
    40501 aaacgcttct cttcataagc atcaagccag gtaaaatcgg gctgattatc cgcaaaaatt
    40561 ccggtcatca gttcgatata cgggccgtta ttgtcggtca gactcttatc ccacgcctgg
    40621 ccaaattcac tgtgtcccca gctccactgt tttttacctg gcgcgatatg gtggttggca
    40681 acgtgtagca aaccgccatc ttcatcgtga caccacgcgc caacaaaatc gtactgtgat
    40741 ttttcagcca tatatgaggt tggaacgggc acatttttat agcgagaaat gtccactccg
    40801 gcggagtagt ccactttgta gtaagtgccg gtggcgatgg ggaaagcaga gacggcccgt
    40861 ttgccgtgat caaacaccgc cgttacatcc ggcgggaaga cgctctgatg cccttcaccc
    40921 cctttcactg ccgggttggc ccaccacaag aaatgacgcg gcgtggcgtt gccgttatag
    40981 acgcggctgg caatttccag tgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc
    41041 tgtaaaccgt gcatcggctc cgtttcgccg acccacacgg tttgtgcgcc gtcttcatgg
    41101 gcttcgaggg tgaaatcgac gggcataaag gtggtcgggc gatggtgttg cggccagtta
    41161 aactcaatcc cgccggagat ccacggcccc agcagtccca ccagcgccgg tttaatgact
    41221 tcattgtgat aaacaaaatc gcgctgtttc actttatccc acgcgcgatg cacccgaccg
    41281 cccagttccg gcaggatcat cactttgatg tagtcgtttt ccagccacac cgcctgccag
    41341 gatttcaggg ttttctgctc gctcagcgta tcggtcacgc cgtagggata aaccgcgccg
    41401 gacgatccct gataaacgcg attttccagg aacatgggat gtatatcctg cggcccggtt
    41461 tcataggtcg ggatctcaac gcgctcttgc cacactttta ctggagtcat ggtgccctca
    41521 atattaacaa gacatactga attaaaagat ttgtggcagt gtattgaaca atctggcaat
    41581 gttttcgcgg aataatcacg caattaacta aacaaggttt agtaaagatg agagcctgca
    41641 ttaacaatca acagattcgc caccataaca aatgcgtgat tctggaactg ctgtaccggc
    41701 aaaagcgcgc caataaatca acgctggccc ggctggcgca aatttcgatt ccggcggtca
    41761 gtaatatttt gcaggaactg gaaagcgaaa aacgggtggt gaatatcgac gatgaaagcc
    41821 agacgcgcgg gcatagtagc ggtacatggc tgattgcgcc ggaaggagac tggacgctgt
    41881 gcctgaacgt gacgcccacc agcattgagt gtcaggtcgc taatgcctgt ttaagtccga
    41941 aaggcgaatt tgagtattta cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa
    42001 tcgaaaaatg ctggcatcgc caccgtaaat tgtggccgga ccgcaccatc aatctggctc
    42061 tggcaatcca cggtcaggtt gatccagtga ccggcgtgtc gcaaaccatg ccgcaagcgc
    42121 cgtgggcaac accgattgag gtgaagtacc tgctggaaga gaagctgggc attcgggtaa
    42181 tggtcgataa tgactgcgtg atgctagcgc tggcggagaa atggcaaaat aattcgcaag
    42241 tgcgtgattt ctgcgtaatc aacgttgatt acggcattgg ctcgtcgttc gtgattaacg
    42301 agcaaattta tcgcggcagt ttgtatggca gtggacagat tggtcacacc atcgttaatc
    42361 cggatggcgt cgtctgcgac tgcgggcgtt atggctgcct ggaaaccgtc gcctcgttaa
    42421 gcgcattaaa aaaacaggcg cgggtatggc taaaatcaca accggttaat actcagcttg
    42481 atcctgaaaa attgactacc gcgcagttaa tcgctgcctg gcagagtgga gaaccgtgga
    42541 tcaccagttg ggttgaccac agtgccaacg ccattggttt gagtctgtat aacttcctca
    42601 acatcctcaa tattaatcag atttggttgt acggtcgcag ttgtgccttt ggtgagaact
    42661 ggcttaatac cattattcgc cagacgggat ttaacccgtt cgaccgcgac gaaggaccga
    42721 gcgtgaaagc gacacagatt ggctttgggc aattaagccg cgcacaacag gtactgggaa
    42781 ttggctattt gtatgttgag gcgcagttac ggcagatttg atggcgcgat aacgtagaaa
    42841 ggcttcccga aggaagcctt ttttcaacag attaaccgtt tcgcttatgc gtaaaccggg
    42901 taacgtgcgc agatgtcgag aactttacct ttgatgcgct cgataacggc ttcatcattg
    42961 atgctgtcca gcacgtcaca catccagcca gccagttctt tcgcttcggc ttctttaaag
    43021 ccacgacggg taatcgccgg agtacctaca cgaataccgg aagtcacaaa cgggctcttc
    43081 ggatcgttcg gtacgctgtt tttgttcacg gtgatgttag cacggcccag agcggcgtct
    43141 gcttctttac cggtcaggtt tttatcaacc agatcaacca ggaacaggtg gttatcagta
    43201 ccaccagaaa ccactttgta gccgcgttcg aggaacactt ctaccatcgc tttggcgttt
    43261 ttcgcgacct gctgctggta agttttgaac tcaggttcca ttgcttcttt cagagcaacc
    43321 gctttaccgg cgattacgtg catcaacgga ccgccctgac caccagggaa aacggcagag
    43381 ttcagttttt tgtacagctc ttcgctacca cctttcgcca ggatcaggcc gccgcgcgga
    43441 cccgccaggg ttttgtgagt ggtggtagta acaacgtgag catgaggaac cgggttcggg
    43501 tagacgccag cagcaaccag gcccgcaacg tgcgccatat caacgaacag gtaagcaccg
    43561 atgctgtcag cgatttcacg cattttcgcc cagtcaacca cgccggaata tgcagagaag
    43621 ccaccgataa tcattttcgg tttgtgttct ttggcttgtt tttccagatc ggcgtagtcg
    43681 atatgaccgg tagcatcgat accgtaagga acgatgttgt acagtttacc ggagaagtta
    43741 accggagaac cgtgagtcag gtgaccgcca tgcgccagat tcatacccag aacggtatcg
    43801 cctggttcca gcagcgcggt gtagaccgca aagttagcct gggagccgga gtgcggctgg
    43861 acgttagcgt agtcagcgcc gaacagttct ttcgcacgat cgatcgccag ttgttcaacg
    43921 atatcaacat actcgcaacc gccgtagtag cgtttgcccg gataaccttc agcatatttg
    43981 ttggtcagct gagaaccctg cgcctgcatt acgcgcgggc tggtgtagtt ttcggaggcg
    44041 atcagttcga tgtgctcttc ctgacgtact ttttcctgct ccatagcctg ccacagttcg
    44101 gcatcataat cggcaatgtt catttcacgc tttaacatcc gcatctcctg actcagctaa
    44161 caataaaatt tttggccttt ataggcggtc ctgttggaca acggcgaaca gtataaccga
    44221 atcattgtgc gataacaggt cttgacaaag gaatttacgc aaacgattac cttcaggcta
    44281 cgcaaggctt tggagaataa agagcttgca accggaaacg gatttctttt caggtttgtg
    44341 atgcaaattt ttcacttcat cacattcttt ctgaaaaaca ccaaagaacc atttacattg
    44401 cagggctatt ttttataaga tgcatttgag atacatcaat taagatgcaa aaaaaggaag
    44461 accatatgct tgacgctcaa accatcgcta cagtaaaagc caccatccct ttactggtgg
    44521 aaacgggacc aaagttaacc gcccatttct acgaccgtat gtttactcat aacccagaac
    44581 tcaaagaaat ttttaacatg agtaaccagc gtaatggcga tcaacgtgaa gccctgttta
    44641 acgctattgc cgcctacgcc agcaatattg aaaacctgcc tgcgctgctg ccagcggtag
    44701 aaaaaatcgc gcagaagcac accagtttcc agatcaaacc ggaacagtac aacatcgtcg
    44761 gtgaacacct gttggcaacg ctggacgaaa tgttcagccc ggggcaggaa gtgctggacg
    44821 cgtggggtaa agcctatggc gtcctggcta atgtatttat caatcgcgag gcggaaatct
    44881 ataacgaaaa cgccagcaaa gccggtggtt gggaaggtac tcgcgatttc cgcattgtgg
    44941 ctaaaacacc acgcagcgca cttatcacca gcttcgaact ggagccggtc gacggtggcg
    45001 cagtggcaga ataccgtccg gggcaatatc tcggcgtctg gctgaagccg gaaggtttcc
    45061 cgcatcagga aattcgtcag tactctttga ctcgcaaacc ggatggcaaa ggctatcgta
    45121 ttgcggtgaa acgcgaagag ggtgggcagg tatccaactg gctgcacaat cacgccaatg
    45181 ttggcgatgt cgtgaaactg gtcgatccgg caggtgattt ctttatggct gtcgcagatg
    45241 acacaccagt gacgttaatc tctgccggtg tcggtcaaac gccaatgctg gcaatgctcg
    45301 acacgctggc aaaagcaggc cacacagcac aagtgaactg gttccatgcg gcagaaaatg
    45361 gcgatgttca cgcctttgcc gatgaagtta aggaactggg tcaatcactg ccacgcttta
    45421 ccgcacacac ctggtatcgt cagccgagcg aagccgatcg cgctaaaggt cagtttgata
    45481 gcgaaggtct gatggatttg agcaaactgg aaggtgcgtt cagcgatccg acaatgcagt
    45541 tctatctctg cggcccggtt ggcttcatgc agtttgccgc gaaacagtta gtggatctgg
    45601 gcgtgaaaca ggaaaacatt cattacgaat gctttggccc gcataaggtg ctgtgatttg
    45661 atgttgccgg atggacacat ccggcaaccc ttgacgcgtt ttaaattgcc gcgtcgtcct
    45721 cttcaccggt acggatgcga atgacccgtg ccacgtcgaa gacgaagatt ttaccgtcac
    45781 cgattttgcc agtttgcgcc gtgcgaataa tggtatcgac acaggtatcg acaatgtcgt
    45841 ccggtacgac aatctcaatt ttcactttcg gcagaaaatc caccatatac tccgcaccgc
    45901 ggtacagctc ggtatggcct ttctggcggc caaaaccttt cacctcagtg accgtcatgc
    45961 cggtaatacc gacttcagcc agtgcttcgc ggacatcgtc cagcttgaag ggtttaataa
    46021 tcgcatcaat ctttttcatg ctattccttg aaaaggtcgc ctgtcttttg atctgctaaa
    46081 cgtaacacat aacgccaatt cattccttga aatcgtttgc atccagttcg tgtcgggaaa
    46141 gcagtttata aaattctgtc cggttgcgcc ccgccattct cgccgcgtgg gtgacgttgc
    46201 ctttggtgat ttgcagcagc ttacgcaaat agttgagttc aaactgatta cgcgcctcaa
    46261 caaaggttgg cagcgccgtg ttttcgccct ccagcgcctg ctccaccagc gcatcactaa
    46321 ttaccggaga tgaggtcagc gccacacact gttcaatcac gttgaccaac tggcgcacat
    46381 tacccggcca gctcgcggtc atcaggcgtt tcatcgcatc ggtggagaac gcgcggacaa
    46441 acggtttatg tcgctctgcc gcctggcgca acaggtgatt tgccagtagc ggaatgtctt
    46501 ctgtgcgctc cgccagtgcc ggaattttca ggctgacaac gttgaggcgg taataaagat
    46561 cttcacggaa ttccccacgc gccatcgctt ttggcaggtc gcggtgagtg gcggaaataa
    46621 tccgcacgtc aatatcaata tcgcggttac tgcccagcgg gcgcacttta cgctcctgca
    46681 acacgcgcag cagtttgacc tgcaacggtg caggcatatc gccaatctca tcaagaaata
    46741 gcgtaccgcc ttccgccgcc tggaataaac cttcgcgatt gctgacagcg ccagtaaacg
    46801 cgccacgtgc atgaccaaac agctccgact ccagcaattg ctcgggtaat gcgccacagt
    46861 taatagcaat aaatggcttg ctgttgcgcg ggctggcgtt gtggatagcc tgggcgaaaa
    46921 tctctttccc ggtgccgctc tggccgttaa tcaaaacgct aacgtctgat tgcgccacca
    46981 gccgcgcctg ttccagcaaa cgcagcatca gcgggctgcg ggtgacaatc gcctcgcgcc
    47041 agcgttcgtc ggtggctggc gcggattgtt ccagcgcatc gtcaattgcc tgatatagcg
    47101 cgtctttgtc gacaggcttg gtgaggaaac tgaacacgcc ctgctgtgtt gcagcaacgg
    47161 catcgggaat agaaccatgt gcggtaagaa taattaccgg cattccaggc tgcactttct
    47221 ggatttcagc aaacagctgc ataccatcca tttcatccat tcgcaggtcg ctgatgacta
    47281 aatctacttt ttcgcgattc agtacccgta atccttcagc gccactctcc gccgtgacca
    47341 cgctgtagcc ttcgctggtc aggcgcaggc caagcagttt cagcaatccc ggatcgtcat
    47401 cgaccaataa taaatgcgca ggtttatggc tcatcaggag tgacctcatg ggtggatggc
    47461 gcgggctttt cactctcgtg cggcgtatcc ggcgagaaat ttccagccgg tttgcgggtc
    47521 gagagctggc gttcaatatc ggtcaggttt tccagcttgc gggtagtgag ttccagttgc
    47581 tgttgtagaa cgtgatgttg ctggcgcaaa gtatccagct cgctgtcgct ggactgctgg
    47641 agtttgctgt agcgttggcg ctcttccgcc agttgcagtt gcagcgcctg accgtcgcgc
    47701 cagagttgat acagtgggcg aacctgtgcc gggatctcgg tacttagcgc atcaatgcgc
    47761 gcgaccagct ggcggcgctc atacggcgta attttggcgt cggcgagcaa aatcccttgt
    47821 ttaaaggtat tttgccagtt gccgtcgtca tattggcggg cttgctgacg cgactgcgca
    47881 ggcattaaac gatcagcaca atccatcgcc cgcagccagt aaagcggatt ggtttcggtt
    47941 gatttacctt gcagcgccca gatgtcgcta cattcagtag aaagatagtc agccagttga
    48001 taaaccggaa ttttttcttc tgctggcgta tcaatggctg gtttattgtg actctgcacg
    48061 caacccagca atgccagaca tggcagccct gccagccaca gccgtcgggg caataatcgt
    48121 tgaaaaatgt gtcgcatatt caccagactt aatgcctatc ccagtgggcg taattgttgc
    48181 aggcagtttg gacatggaca gcgcggagaa accggagcat acacgtcgta cgtgaggatt
    48241 tcgagcactg cccggggccg aactggcaaa taaaatagcc tgatgggata ggctgttagc
    48301 attatttcgt gtttttcgac gacggtaatt caatgcggaa gcaaacgtct tgcccgctct
    48361 cgtcgaccag atacagttcc ccttgcatac ggcgaataca atccctggca atgcttaatc
    48421 ccagaccgct gcccttcacc gccccttttc gctggtggct tccttgaaaa aagggttcga
    48481 agatcatggc gcgttcctct tgcggaatgg gtgtgcctgt attgatgaca tcaatataaa
    48541 cccgcgcacc atgtaagctg ctgcgaaggc aaatgttacc ggattcagcc ccgtagtgca
    48601 cagcattgga gtaaagatta tccagtacgc tcatcagcag cattggctcc gccaggcaag
    48661 ctgttgcttt gagatcgacg tcggtatgca tcattttagc ccgtgcgggc aggctatgag
    48721 cagaaactac tgtctccacc agcggtgcta attcaacatt ctccagttcc accgcactgt
    48781 ccgcctgttt acggttgtaa tcaagcagtt gttcgatcag cttttgcaaa ttgcggctgc
    48841 tgctatcaag aatgctcacc acctcttttt gctctggcgt aagcggcccg acaacctgat
    48901 cagccagtaa ttcagtgccc tcgcgcatac tcgccagtgg cgtttttaat tcatgagata
    48961 aatgtcttaa aaattgatgg cgttgggatt ccagccatga caggcgctca cttaaccaaa
    49021 gaatacgttg cccaaccgag cgtaactcgc tcggtccact gaacgagacg ctattgccca
    49081 gagaacgccc ttcccccagc cggttgatca tgcgctcgat atttttcacc ggaccgataa
    49141 tcatcctcgt gaaaagcagt accatcacca gactcaccag aaatagcacc agcgattgcc
    49201 aaccaaaata ttgcccacgt tcggcgattt cacgctgaag ttgctgccca cgagagaaca
    49261 ccactgtgcg cgtggcctgc accatttcgg tattggcact ggcaaaggct tccagacgcg
    49321 cggcggcagc agcatcggga ccgctgttgt tacactgaag ttgagccaga ttgttcaaat
    49381 cctgacgtaa tgcctggtag agtttatcgt ccggcagcac gcctgcgtgg gcatcgagca
    49441 tttcgctgta acgcttgcgc tggctttgat aaaccttcgc aagcgtaggg tcgtccagca
    49501 cgcagtactg acgatagctg cgctccatct ccagtgccgc gttggtcatc gcttcactgc
    49561 gccgggcatc aataagcgta gtgcggttaa ccagcgccgc ctgatcgcta agtgcattca
    49621 ggctttgcca ggcttgccat gccagcacca acaggggcag cagaatcagc aaaaatgcca
    49681 gcattaccag ttgtcgtaat gagcggggaa aaacgggcca gcgtttcaag gcgttactct
    49741 cgtcagacgc gaatagcctg atgctaaccg aggggaagtt cagatacaac aaagccggga
    49801 attacccggc tttgttacgg aataaggcgg tgcctaactc gacgtttcgc ccgatggttg
    49861 atatagctac gctgatatca gaagttggac ggcaggcacc ttgttgtgcg tcattcgaat
    49921 tttatgtagc acgtcccgaa ggggctgaca taagtcggcg aatgagccac tgcttactat
    49981 tatgcagtaa ctgtgccaat aaagaaaata gtttggtaac atactgatta tacgttgctt
    50041 tgaggggttt atggcttctc cgctatttga atgtaaatca gccactgtgt cgctaaaaag
    50101 agacaactta agataaactt attagataat attaaaatca atgagttaag tgtcaccaaa
    50161 aagcgacacg gcaaaccacc cattgtcgtg atttacagac acaaaaaagc tcccgaagtt
    50221 gggagctcat aatagcggtt ggtgcttaat gccggatgcg gcgtgaatgc ctcatccggg
    50281 ctgcaatacc aatgggttga cgacttaccc caactgctta cgtgcattgc ggaaaatgcg
    50341 catccatggg ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa
    50401 aactcgttcc gggtgcggca tcataatggt gactcgacca ctttcagtcg tgaccgccgt
    50461 aataccgttc ggtgaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt
    50521 atcgacatag cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc
    50581 gcgcacttca acgcgccctt caccgtgaga gacagcaatc ggcatctgcg agcccaccat
    50641 cccctgcaac agcagcgacg ggctttgggt tacttctacc agactgaaac gcgcttcaaa
    50701 gcgatcggag gtgttacgca caaaacgtgg ccacaactcg ctacctggga tcagttcacg
    50761 cagattagac atcatctggc aaccgttaca tacccccagc gccagcgttt gcggacgatg
    50821 gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac aggatagact tcgcccaacc
    50881 ttcaccagcg cccagcacat caccgtagga aaaaccaccg cacgctacca ggacgtggaa
    50941 accctccagg cccgtgcgtc cagccagcag atcgctcata tgcacgtcga tagcatcgaa
    51001 gcccgcacgg tggaaagctg ccgccatttc aacatgggag ttcacaccct gctcacgcag
    51061 tacggcaact ttcgggcgcg cgccagtggc aataaacggt gctgccacat cttcgttgat
    51121 atcaaacgac agtttcacat tcaggcccgg atcggcgtcg tttgatttcg cctggtgttc
    51181 ctgatcggca cactccgggt tgtcacgcag acgctgcatc tgccaggtag tttctgccca
    51241 ccagacacgc aacgtggtgc ggctttcgct aaatacagtc tgcccgttgg cggtaatcac
    51301 aaaacggtca ccggaaaccg cctgcccaac ataatggacg caatcagcaa gcccatgctg
    51361 tgccagtacc gcttcaacgg cttcacggtc agcggctcga acctgaatca ccgcacccag
    51421 ttcttcgcta aacaatgcag ccaggcgatc gtcacccaga gtggcgatat ccgcgtcaat
    51481 gccacaatga ccagcaaagg ccatttccgc cagcgttacc agcaggccgc catcagagcg
    51541 gtcgtgatac gccagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc
    51601 tttcagttgg gcaacatcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg
    51661 cgccagtgcc gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcaacagtgc
    51721 gttatcttcg gtagaaagct gcggcgtaat ggtgtgacgt acatcttcca cgcgggcaaa
    51781 tgcggaaatc accagcgaca acggcgacgt catttcgcgc tcttcgttac cttcctgcca
    51841 gcgggttttc atcgacatag agtctttacc caccggtatc gtcaggccca gcgccggaca
    51901 aagttcttcg cccacagctt taacggcttc atacaggccc gcatcttcac cagggtggcc
    51961 tgccgccgcc atccagttgg cggaaagttt aatgcgtttg atatcgccaa tttgtgttgc
    52021 ggcgatgttg gttagcgctt caccgactgc cagacgggca gaagcggcga aatccagcag
    52081 cgcaactggc gcacgttcgc ctattgccat cgcttcaccg tagtagctgt cgaggctggc
    52141 ggtagtgact gcgcagttag cgaccggcac ctgccacgga cccaccatct gatcgcgcgc
    52201 caccatgccg gttacgctgc gatcgccgat ggtcaccaga aaggttttct ccgccacagt
    52261 cggcagatgt aacacacgtc tcaccgcgtc agcgatggtg atcccttcac gggccagcgc
    52321 gtcgcctttc gctttcagcg tttgcacatc gcgggtcatc ttcggcgttt tgccaagcag
    52381 tacgtcgagc ggcagatcga tgggttgatt atcaaaatga cgatcgtgca gagaaagatg
    52441 taattcttcg gtggcctcac caatcaccgc gtacggtgcg cgctcacgct tacacagttc
    52501 gtcaaacagc ggtaactgat cggcagcaac tgccagaacg tagcgttcct gggattcgtt
    52561 acaccagatt tccagcgggc tcatgcccgg ttcgtcgctc aggatatcgc gcagttcaaa
    52621 tttaccgccg cgtccgccgt cgctcaccag ctccggcatg gcgttagaaa gaccgcctgc
    52681 gccgacgtcg tggataaaca gaatcgggtt cgcgtcgcca agctgccagc agcggtcgat
    52741 cacttcctga cagcgacgtt ccatttccgg gttatcgcgc tgaacggacg cgaaatcgag
    52801 atccgcgtca gactggccgg aggccatgga agaggccgcg ccgccgccaa gaccgatgtt
    52861 cattgcgggg ccgccgagaa cgaccagttt cgcaccgacg ttgatctcgc ctttttgtac
    52921 gtgatcggcg cgaatgttgc cgatcccacc cgccagcatg atcggtttgt gatacccgcg
    52981 cagctcttcg ccgttgtggc tgttcacttt ttcttcatag gtacggaagt agccgttcag
    53041 tgccggacga ccaaattcgt tgttaaatgc cgcgccgccc agcgggcctt cggtcatgat
    53101 atccagcgca gtgacaatgc gctcaggctt accgaaatct tcttcccacg gttgttcaaa
    53161 gccaggaata cgcaggttgg aaacggagaa accaaccagg ccagctttcg gcttcgcgcc
    53221 gcggccggtg gcaccttcat cgcggatttc accgccggaa ccggtagctg cgcccggcca
    53281 cggagaaatc gccgttgggt ggttgtgggt ttctactttc atcaggatgt gcgccggttc
    53341 ctgatggaag tcgtagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcggaacc
    53401 ttccattaca gcggcgttat ctttataagc agagagaacg tgatccggcg tggtttcgaa
    53461 ggtgtttttg atcatcttaa acagcgattt cggctgctgt tcaccatcga taacccagtc
    53521 ggcgttaaaa attttgtggc ggcagtgctc ggagttcgcc tgggcaaaca tatacagttc
    53581 gatgtcgttc gggttacgac caagctttgt gaacgcatcc tgcagatagt caatttcatc
    53641 ttctgccagt gccaggccaa gacgcaggtt agcgtcgatt agcgcctgac ggccctgccc
    53701 cagcaaatca acgctggtga ccggagtcgg ttgatggtgt gcaaacagct gctctgcatc
    53761 atctaaagca aaaaagaccg tttccatcat gcggtcgtgc agttcagcgg taacctgctg
    53821 ccattgttca ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg
    53881 gtttacctgt tgtagaccgc aattatgggc gatatcggtc gctttcgaag accagggaga
    53941 gatggtgcca ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag
    54001 tgccgggcca tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc
    54061 attgaggtca gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc
    54121 ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac aatgcaggcg aaccacgcag
    54181 aatttccatc ataagtctct cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg
    54241 gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca
    54301 tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt
    54361 acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt tttcaagctg
    54421 ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca
    54481 tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta
    54541 aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg agcaccattc
    54601 atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg
    54661 cgaaacagtt tgccgattac ctcggtgtaa aactgaaagt gaccgtgcgg cagaatacca
    54721 gccagctgtt tgacgacctc gataatggta acgccgacct gctggcggca ggactggtct
    54781 ataacagtga gcgggtaaaa aattatcagc ctggtcctac ctattattcc gtgtcacaac
    54841 aactggttta taaagtcggt cagtatcgcc cacgtacgct gggcaacctg acggcggagc
    54901 aactcaccgt tgcaccgggt cacgtggtgg ttaacgatct ccagaccctg aaagacacaa
    54961 aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag
    55021 atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc
    55081 agcgcgttca cccggaactc gccgtagcgc tcgatatcac cgatgaacaa ccggtgacct
    55141 ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg
    55201 aaatgaatga agacggtaca ctggcacgca ttgaagagaa atacctgggg catggcgatg
    55261 attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt
    55321 taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg gccgctattg
    55381 cttatcaaga atcgcactgg gatgctcagg ccacttcacc gacgggtgtg cgcggcatga
    55441 tgatgttaac caaaaacacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac
    55501 agagcatcag cggcggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg
    55561 tgccggagaa cgaacggatt tggtttgccc tcgccgcgta caatatgggc tatgcgcata
    55621 tgctggatgc ccgcgccctg acgacaaaaa ccaaaggtaa tcctgacagt tgggctgacg
    55681 taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct
    55741 acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaaatat cagattagcc
    55801 tggtgggtta tctgcaggag aaagagaagc aggctacaga agcggcgatg caactggcgc
    55861 aggattatcc ggcggtatca cctacggagt tgggcaaaga gaaatttcct tttctctcgt
    55921 ttctttccca gtcgtcatca aactatttga cccactctcc ctctctgctg ttttccagaa
    55981 aagggagtga agagaaacaa aattaatctg tcgaggattg cgcttttttc tgcgctttaa
    56041 tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcacactca tccgccagta
    56101 ttccttccgt aatttccacc cggtgattca tacccggatg atgcagcaca tccattaaag
    56161 atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt
    56221 ggatcattgc tccggcacac attacacagg gttcaagcgt gacatacaac gtggcgtcga
    56281 tcagacgata attttgcatc accagaccgc cctgccgtag ggccatgatt tctgcatgtg
    56341 cggtgggatc atgtcgacca atcggacggt tccagccttc gccaataaca cgattgttat
    56401 gcactaatac cgcgccgacc ggtacttccc gctcatccca ggcacgcttc gccagcgtca
    56461 ttgcatgacg catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccga
    56521 ttataaaagc gcggcgcatt atacacggcc gctatgcttt actctattcc agttgctgga
    56581 gttcaccgcg cggggttact cgccaacgat gctgacagaa ataaagcagc gggttgtcct
    56641 gattactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg cgctccagtt
    56701 gcgcgacctt ctcatgcccg aggcaacgca tcgtcaatac ccaaccacca tagccacgct
    56761 gaatctgact ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt
    56821 caaccagcga ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt
    56881 aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcacga aaccaacgta
    56941 cgaaatcggc ctgcaacgcc tgcaaacgcg cttcattgtg accaaaagtg catccccaca
    57001 gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca atggctatga
    57061 tcggcaacaa cggcagaaca agtaacgcat tcagcggttg gcgacgcagt aaataacgca
    57121 aaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac
    57181 gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc
    57241 tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc
    57301 taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtt
    57361 tgtctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac
    57421 ttcgtatccg tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc
    57481 tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg
    57541 gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg
    57601 cactaaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatctccc tccgtgccca
    57661 ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag
    57721 aaaatactgc cgctatgtac gcaacgttaa acgttaatag tgaagagaaa ctgcatgaat
    57781 gcgtggcaat gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc
    57841 tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc
    57901 gcgatatgca tgcgctactc gctacagtac aggcgtcgtc ccctgacgat ctgttattag
    57961 ccatttccta caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag
    58021 tgggcggaaa agtgctggct attaccggct ttactcccaa tgccctgcaa cagcgtgctt
    58081 ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt
    58141 gtcacgcgca gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg
    58201 aactggcacc agaacgtatt cgtcatagcg aagcgctggt gaaaaaactg gtctgagtaa
    58261 agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgctg
    58321 ttaatcacta aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg
    58381 atttcgatgg gtgaacatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg
    58441 cactacgaga caccaacctg ccagaaggtg tgtccgatcc ccaatactat tgtgaaagat
    58501 ccgcaacatg tcgagacgga agaacagttg tgggataaat ttgtgctgat gcaccacgcg
    58561 gataaaattt aatattctgc tctggaagta gagtgttaat tattttatag gaaaatctta
    58621 acgctattat atttatttaa atgatgacat tagcataatc attcactaag ttaatttata
    58681 tagtatctgc ccagacactt atttatagtt attaaaggcg cgtccgactg gttcaccgga
    58741 cgcaccttaa gtacgtttcc ttgtgttata agaacaaaag gagcagctgt aaaacagcaa
    58801 tgatgatttt gatgacccgt ttaatcaggt atcggcaatc agtcattcgt ttttccttaa
    58861 acaaggaatg cagccgttca gattagccct tacatctccc caaaactgaa cgtgcgagtt
    58921 attggaatcg agtgctgcac tccacaccag agctttgacg acaccactcg tttcaatggg
    58981 ggaattcagt ggcatggtgt aaagcacagc aaaattgcca ataacgaaac cgattttaat
    59041 gtacttatca aaaagcggtc acgttaatct tttcatcaat gtcgatttct tcgtaaaggc
    59101 tcgcgttttt tatgtcagag attgcaagtt gcttgtacag gtcaacgaaa ttgatccata
    59161 atcattttcg ttggaatcgt gtgctgcaca aaattaaagt taaaaagtaa aacccccgtt
    59221 ccttaccagt tcgggggttt tactttataa agagaacggt attattttaa ctttcaataa
    59281 ttaccgtggc acaagcatag tggcgctcat cggcaagcgt tacatgcata tttacaacgc
    59341 cgagcttttc cgccagtttt aatgcctcac cccatagccg tagccgtggt ttgccgagct
    59401 catcattgaa tacttcaaac tgattaaacg ccagaccatt gcggatccct gtgccaaatg
    59461 cttttgccgc cgcttctttc acagcaaaac gcttcgccag aaaacgcact ggctggtggt
    59521 gcgttttcca gatttcccat tcgttatcgc ttaatacgcg gcgtgccagg cgctcaccgg
    59581 atcgggcgat caccgcttct atgcgagcga tctctacaat atccgtacct aaacctaata
    59641 ttgccattag ccacgcgctt ccagcatcag acgtttcatt tctgccaccg catctttcag
    59701 tccggtcatc actgcacgac caataatggc atgaccgata ttcagttcat gcatctcagg
    59761 gatggcggca atggctttca cgttgtgata ggtcagaccg tgtccggcgt taactttcag
    59821 accgagacta gtggcaaagg tcgcggcttt ggcgatacgc gccaactctt gcgcctgttc
    59881 ggcgtcagtt ttggcatcag catagcaacc ggtgtggatc tcgataaacg gtgcgccaac
    59941 ctctgccgca gctttgatct gatcttcatc ggcgtcgata aacagagaaa cctggatccc
    60001 ggcatctgcc agacgtttgc aggcatcgcg cattttttca cgctgccctg cgacgtccag
    60061 gccgccttcg gttgttactt cctgacgctt ttccggtacc agacagcaaa aatgtggctt
    60121 cgtctcaacg gcgatcgcca gcatctcttc ggtcaccgcc atctccagat tcatgcgggt
    60181 atccagcgtc tggcgcagga tgcacacatc gcggtcggta atgtggcggc ggtcttcacg
    60241 taaatgcacg gtaatgccgt ccgctcccgc ctgctcagca ataaacgccg cctgcaccgg
    60301 atccgggtaa gcagtaccgc gcgcgttacg cagcgttgca atatggtcaa tgttgacgcc
    60361 taacagtaat tcagccatga caatcctcat cattcataat gtgttttcac cgttcgctta
    60421 ggcataaact gccggaacag ttccctgctc tttaatggtt taccgccaag atacggctta
    60481 agcgccatgc gggtaaagcg tttcgcggcg cgcagtgtgt ctgcgtcagg aaattcccgc
    60541 gcgtttaacg cttttaactg ccttccggtg aacgttttat tgtcgataac gacgcttgcg
    60601 ataaaccctt tttcttcgcg ataacgatac gtcatggtgt catctaccgg ctcgccgcta
    60661 cccgcacaat gggtaaaatt gacgccatag cccagatgcc cgagcagtgc cagttcaaag
    60721 cggcgcaacg cgggttctgg cgtaccagtg accccggcaa gagactgaat gcagtgcaag
    60781 taatcgaaaa agagttcaga gaagcgcgtc tcgtattcca gtacgcggga gagaagttcg
    60841 ttgatgtaca gaccgctgta aagcgtgata ccgcttaatg gcagcgccag cgagacagct
    60901 tcagcactgc gcagcgtttt gacttcgcca cgcccgccaa aacgtagcaa gagaggggtg
    60961 aaaggctgta atgccccttt cagagtagag cgtttagagc gtgcgccttt ggcaaccaga
    61021 cgcacgcgac ccgattcctc cgtgaagacg tccagcatca ggctggtttc gctccacggg
    61081 cgactatgca ggacaaatgc gcgctgccag ccttccatcg gagttactct taaagatcgt
    61141 caacataacc gagactgcgc agtgcgcgtt cgtcgtcggc ccaaccggat ttcactttta
    61201 cccacagctc aaggtgaaca ggcgcttcga acatttcctg catgtcttta cgcgcttcaa
    61261 tcccgatggt tttgatcttg gcccctttgt tgccaatgac catcttcttc tgcccttcac
    61321 gctcaacgag aatcaaaccg ttgatgtcat aaccgccgcg ttcgttagag acgaaacgtt
    61381 cgatctccac ggtcacggag tacggcagtt cagcgccgag aaaacgcatc agtttttcgc
    61441 ggataatttc agacgccata aaacgctgtg agcgatcggt gatgtaatct tccgggaagt
    61501 gatgggtcgc ttcaggcaga tgcttacgca cgattgccgc gatagtgtcg acattcagcc
    61561 cggtttcggc agagatcggc acgatatcga ggaagttcat ctggcttgcc aggaactgta
    61621 agtgtggcag cagatcggct ttttcctgca cgttgtccac tttgttcacc gcgaggatta
    61681 ccggcgcttt gccatcacgc agcttgttga gcaccatttc atcgtccggc gtccagcggg
    61741 tgccttctac gacaaaaatc accagctcaa cgtcgccaat ggagctgctc gccgctttgt
    61801 tcatcagacg gttaatagcg cgtttttctt ccatatgcag acctggggta tcaacgtaga
    61861 tcgcctgata cgcgccttca gtatggatcc ccacaatgcg gtgacgagtt gtttgcgcct
    61921 tgcgggaggt aatggagatc ttctgcccca gcagtttgtt caacaatgtg gatttgccaa
    61981 cgttcggacg tccgacgata gcaataaatc cgcagtaact tttatcgatg ctcattccag
    62041 ctccagtttt ttcaacgcct gttcggcggc agcctgctca gccttacgac ggcttgaacc
    62101 tgtgccaacc accggttcac tcaggccgct gacctggcag tggatagtaa attcctgatc
    62161 gtgcgcttcg ccacgtacct ggactaccag ataagttggc agcggcagat ggcgaccctg
    62221 caaatattcc tgcagacgcg ttttcggatc tttttgttta tcgcctgggc taatttcgtc
    62281 cagacgagtt tgataccagt tgaggattaa tttctcgacg gtttgaatat cactgtcgag
    62341 gaatacgcca ccaattaatg cctcgacggt gtcggcgaga attgactcac gacgaaatcc
    62401 accgctttta agttcacctg gccctaaacg taagcactca cctaactcaa attcgcgcgc
    62461 cagttcagcc agcgtattac cacggaccag cgtggcgcgc atccggctca tatcgccttc
    62521 atccacacga gggaaacggt gataaagcgc attggcgata acgtagctca gaatagagtc
    62581 acctaaaaat tctaaacgct cgttatgttt actgctggca ctacgatgag ttaatgcctg
    62641 ctgcaacagt tcctgatgat taaaagtgta gcccagcttc cgttgaagcc gattaattac
    62701 gatggggttc atgcgatacc aataaataaa tgcgtcaaca attcagcaca cgaaacagac
    62761 ctgatataca tggctcggct aactgcttca ctgcagtttc agtttaccgg tatatgggga
    62821 ccaacgctgt ttcgtgtgcc gtggcaacct ggaggtgcca accttaaact tcgggggaat
    62881 attctataca caacgacggg ggatgtcgtt agccacggga gatttatctc ataaataatt
    62941 cacgttgtcg ccataacggc gacaacgtga acgaagatgg ctattaatgg ataccgccaa
    63001 tgcgacttaa gcgcacacca gtcggccatt cgccttcttg cttatcgaag ctcatccaga
    63061 tagccgttgc ccgaccgacc agattcgctt caggcacaaa accccagtaa cggctgtccg
    63121 cgctgttgtc gcggttgtcg cccatcatga agtattgtcc cggcggaaca atccaggttg
    63181 ccagttgttg ccctggctgc tggtaataca tccccacctg gtcctgcgca atcggcactg
    63241 tcagaatgcg gtgcgtcaca tcacccaatg tctctttacg ctcggaaaga cgaattccat
    63301 tttctttggt ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc ccaccattac
    63361 ggcgtgagaa ggtctgaacg aaatcgctcg gttccacgtt ggagtaagtg accggtagcg
    63421 cgttttcgca cgcctggccg gaactgcatc ccggttgaat cgtcagctct tttgagaccg
    63481 gatcataagt gactttatcg cccggcaaac ccaccgcgcg cttgatgtaa tcaagctttg
    63541 gatcttccgg atatttaaag accacgatat cgccgcgttt cggatgaccg gtttcgatca
    63601 gcgttttctg gtagatagga tctttaatgc cataagcaaa cttctctacc agaataaaat
    63661 caccgattaa cagagtcggc atcatcgaac ctgacgggat ctggaacggt tcataaataa
    63721 acgaacgcac aatcaatacg atagccagta ccggaaaaac agaagcacca gtttccagcc
    63781 agccaggctt cggcgcaacc tttttcagcg ttgctttatc cagtgagtca ccggcagccg
    63841 cctgcgccgc tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta tccacgcacc
    63901 ataaaatgcc cgtcaccagt gtggcaatca ccagaatcag ggcaaacata ttcgccatgc
    63961 caactcctaa gggttatttg ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct
    64021 gcggcagctc gacgttaccg atctgcttca tgcgtttctt accttctttc tgcttctgca
    64081 gcagcttttt cttacggctg atatcgccgc cataacattt agccagtacg tttttacgca
    64141 gctgtttcac ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga atggcgatat
    64201 caaactgctg gcgtgggatc agatctttca tcttctccac cagctcgcga ccgcggtttt
    64261 gcgaattacc acggtgggtg atcagcgcca acgcatcaac acgttcaccg ttgattaata
    64321 cgtctacacg taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc agagacgcat
    64381 aaccacgcga ggtagatttc aggcgatcga agaagtcgag caccacttcc gccatcggga
    64441 tctcgtacgt cagcgccacc tgattaccgt ggtaaaccat attggtctgc acgccgcgtt
    64501 tttctacgca cagcgtaata acgttgccga gatatgcctg cggtagcagc atgtgacact
    64561 ctgcaatcgg ctcgcgcagt tcgtaaatgt tatttaccgc tggcagcttg gatgggctgt
    64621 cgacgtagat aacttctctt gacgtggttt caacttcata cactacagtc ggcgcagtgg
    64681 tgatcagatc cagatcgtat tcacgttcca gacgttcctg gatgatctcc atgtgcagca
    64741 ggccaaggaa gccacagcgg aaaccaaagc ccagcgcgct tgagctttcc ggctcataga
    64801 acagtgaggc atcgttcagg ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt
    64861 cggaacttac cgggaacaga ccagcatata cctgaggttt gactttctta aagccaggca
    64921 gcgccttttc tgccggatta cgcgccagtg ttaaggtatc gcctactgga gcgccgtgga
    64981 tatctttaat cgcacatacc agccagccta cttcgccaca tttcagttca gtacggtcaa
    65041 cctgcttcgg cgtgaagatg cccagacgat cggcgttata ggtctgcccg gtgctcatga
    65101 ctttcacttt gtcgccctta cgcagggtgc cgtttttaat acggataagt gaaacaacgc
    65161 ccagatagtt gtcgaaccag gagtcgataa tgagtgcctg caacgggcct tccggatcgc
    65221 cttccggcgg cggaatgtcg cgcacaagac gttcgagaac gtcctgcacg ccaacgccgg
    65281 ttttcgctga acagcgcacc gcgtcggtgg cgtcgatgcc gacgatatct tcaatttctt
    65341 ccgccacgcg ttcaggatcg gctgccggca ggtcaatctt gttcagtacc ggcaccactt
    65401 cgagatccat ttccatggcg gtgtagcagt ttgccagggt ttgcgcttct acgccctgcc
    65461 cggcatcgac caccagcagt gcgccttcac aggcagccag cgaacgggaa acctcatagg
    65521 agaagtctac gtggcccggg gtgtcgataa agttaagctg ataggtttcg ccatcagacg
    65581 ctttgtagtc cagcgtcacg ctttgcgctt tgatggtaat gccacgctca cgctcaagat
    65641 ccatggaatc gagaacctgc gcctccattt cacggtcaga caggccaccg cagatctgga
    65701 taatacggtc agacagcgtc gatttaccgt ggtcaatgtg agctatgatc gaaaagttac
    65761 gtatattctt cataaagtat gattattgtg ccttaatgcc cggttaacca ggcttttaga
    65821 agtcgctgtt ctgagcttaa cgtctgatta atagaaacgc cgcattctac actacaacat
    65881 tgaggcgagg aaatgttcat accgtatgga ttgtggtatc tggaaacgtc ctcgcatttg
    65941 ttatgcaaaa tgcaacaaag ccagtgaaat cactggctcg cgtcttccga agatgtttca
    66001 aatcgcacaa ggccaggcgg caatgccacg cttaagatga tcggttgcca ttctgcccgg
    66061 gcagcaaact tacgcgaata gccgcgcgca atcaggaacc cgccaatacc gccgaggatc
    66121 gcaccacata atgctgcgac gtcagaagca aagagtagct gaaatagcga agcgatgagg
    66181 aataatccca ccagcggcga catataaacc agtaatgcgg agctaagaag gctgccttcg
    66241 gcgatcccca attccacttt ttgccccggc actaacggct catcacaggg tacgacaatg
    66301 gtatgcgtgg tttgcgggcc aagtttattc aacacacggc taccgcaacc ggcgcgtgaa
    66361 gcgcagctgc tgcatgaggc tttaacatca caactgacca gcgcctgccc gttttcccag
    66421 gagacgacgg tagcccactc tttgatcatt gcgctgtccc gaacttaata ttctcggcaa
    66481 tgcgtttcgc cgtttgcggc ggcagttcac caacaatggt gatttcggcg ttatcacgta
    66541 cgcttgtact gacggttctg cgcccggtgc gcaacatctg atcggtgctc gatggcgtag
    66601 cgcggttaac gtttaccgag aagctgaata atccgtcgga atagagacgt gattcgatag
    66661 gcatgttgtc catcgtcggt agcggacgtc gactactgga aacttcgcta aaaccctgtg
    66721 gcagccaggt tggcgtccag ttgaatttag ctttttcacc gacaggaaca gaaagcaacg
    66781 gcggcaaatt tgccttcgcc agcgtctgca tactgctgcc gatatcctga ttgacgttaa
    66841 aagcaatcac gcgaaattgt tccagcgttt caccatcgcg atcaaggaga tcaaccctca
    66901 tcggtaattt cgattcggtg tccatccaca cgatgtagct gtagcgtgta ccatctcggg
    66961 caaccacgcg aatgacttcg caaagacgat cagcaatacg cgtgcgcccg acggagataa
    67021 agtcgtagta aggagaaagg cgtttgaaat cggtatagat gagcgatggc agagaatcaa
    67081 caatgtaatc gccattaagc gtgaacggtt caagtcccgg ttcaaaatag ctgatttcat
    67141 tgccgcgctg taccacttcc cggcgcgggc catccatttg caacaattgt gcaagaggac
    67201 ggttatcgag gcgtgcatgt cgataacgca gagactcaac accctgttta ttgatgctga
    67261 taaatgacag ctcgtaattc agtgactgac tggccaggtt catctgctgt aataacgccc
    67321 cggacgcggg agtggccgag gcgttagcag agaataacag gctacctgtc actaatgaca
    67381 tggcaaacca aagttgcttc attactgcga ttgcgttcct aaagtttgaa ttcctggcac
    67441 ctgtacagcg gcttgctgag tttgtgcctg ctcaaactga agctgttcag agtggagtcg
    67501 gcgttgcagt tcgtaatcct gcaacattgc attaatgcga cgacgctgct cctgtacctg
    67561 ctgctgttga ccattatttg cggtcgcttc agaaggtact cccagactaa ccgggctggc
    67621 tttacccatc atcggcagtg tattaaatac tggcgtttcg ggctgctggg acgtttcaga
    67681 ttgcccatta tagtgctgga cgccaacgat aactgcaagc gatacgcatg ctgctacgcc
    67741 catttgggta agctgtgccg cccacggacg tactttctgc cagaatggca ttttctgcca
    67801 ttgatgcggc gcaggctggg cttccgggat caatgtcgcc ggttgacgta ctggctcttc
    67861 ttcaatggcg gccatcacac gtgaagagat atcgaaatgg agcacctcgg gagtatcacc
    67921 ccgcattgag tcacggatca agtgatagct ttcccaggtt ttctgcattt ctgggttatg
    67981 agccagttcg ttaagcagct cactatccag cgtttcgcca tccattaaag cggaaagttg
    68041 ttctttctgc atgcctaata cccttatcca gtatcccgct atcgtcaacg cctgataagc
    68101 ggttgaactt tgttatcaat agcttccctc gctcggaaga tacgtgaacg caccgtacct
    68161 accggacaat ccatgatagc ggctatctct tcatagctca ggccatccag ctcccgcaag
    68221 gttattgcca tgcgtaaatc ttccgggagg gactcaatag ttcggaaaac tatctgtctc
    68281 agttcttctg acaacattaa gttctcaggg ttcgaaattt ctttcaacgc gccgccactt
    68341 tcgaagtttt cagcttcaat ggcatccaca tcactggaag gtggacgacg cccctgagca
    68401 accaggtaat ttttcgctgt atttacagca atccgataca gccatgtata aaaagcgcta
    68461 tctccccgga acgaatccag cgcacgatag gctttaataa aagcttcttg taccacatcg
    68521 ggaacatcac ccgacggcac atagcgggaa accagactcg ccactttatg ctgatagcgc
    68581 actaccagta agttaaaggc tttctgatct cccttctgga cccgttcaac caggacctgg
    68641 tccgttaact gctcgctcat ccgaggtaaa gtctccccaa accaaatttc cacgcgctat
    68701 cgaaacgcca ctccattagc tgcaatttga gcaagcaaag ggttagagtg tctcgttttt
    68761 gtaaagttcc gtaacgcatc tgtttttgtt tgtcatgctg tagacggatc attatctatc
    68821 attataagtc tacagaatct gaacatcgca ttatctgtgt agaaatgccc atttaactgc
    68881 ctgaagagta acccaacggc ctttttattt caccacctaa tcctccacca gccagtaact
    68941 tctcgttttc tcgccgccct gaggcagcgt gtttagtcac tgtaacaaat attaaaatag
    69001 caggtgttta ttcgcacaac atgatgttat gctgaccaaa ccgtgtttag taaattaaac
    69061 aaagaaaatg aatactctcc ctgaacattc atgtgacgtg ttgattatcg gtagcggcgc
    69121 agccggactt tcactggcgc tgcgcctggc tgaccagcat caggtcatcg ttctaagtaa
    69181 aggcccggta acggaaggtt caacatttta tgcccagggc ggtattgccg ccgtgtttga
    69241 tgaaactgac agcattgact cgcatgtgga agacacattg attgccgggg ctggtatttg
    69301 cgatcgccat gcagttgaat ttgtcgccag taatgcacga tcctgtgtgc aatggctaat
    69361 cgaccagggg gtgttgtttg atacccacat tcagccgaat ggcgaagaaa gctaccatct
    69421 gactcgtgaa ggtggacata gtcaccgtcg tattcttcat gccgccgacg ccaccggtag
    69481 agaagtagaa accacgctgg tgggcaaggc gcagaaccat ccgaatattc gcgtgctgga
    69541 gcgcagcaac gcggttgatc tgattgtttc tgacaaaatt ggcctgccgg gcacgcgacg
    69601 ggttgttggc gcgtgggtat ggaaccgaaa taaagaaacg gttgaaacct gccacgcaaa
    69661 agcagtggta ctggcaaccg gcggtgcttc aaaagtttat cagtacacca ccaatccgga
    69721 tatttcttct ggcgatggca ttgctatggc gtggcgcgca ggctgccgga ttgccaatct
    69781 cgaatttaat cagttccacc ctaccgcgct gtatcaccca caggcacgca atttcctgtt
    69841 aacggaagca ctgcgcggcg aaggcgctta tctcaagcgc ccggatggca cgcgttttat
    69901 gcccgatttt gatgagcgtg gcgaactggc tccgcgcgat attgtcgccc gcgccattga
    69961 ccatgagatg aaacgcctcg gcgcggattg tatgttcctc gacatcagcc ataagcccgc
    70021 cgattttatt cgccagcatt tcccgatgat ttatgaaaaa ttgctcgggc tggggataga
    70081 tctcacgaaa gaacccgtgc cgattgtgcc tgctgcacat tatacctgcg gtggtgtaat
    70141 ggtggatgat catgggcgta cggacgtcga aggtttgtac gccattggcg aagtgagtta
    70201 taccggctta cacggcgcta accgcatggc ctcgaattca ttgctggagt gtctggttta
    70261 cggctggtca gcggcggagg atattactag gcgtatgcct tatgcccacg acgtcagtac
    70321 gttaccgccg tgggatgaaa gccgtgttga gaaccctgac gaacgggtgg tgattcaaca
    70381 taactggcac gagctacgtc tgtttatgtg ggattacgtt ggcattgtgc gcacaacgaa
    70441 gcgcctggaa cgcgccctgc ggcggataac catgctccaa caagaaatag acgaatatta
    70501 cgcccatttc cgcgtctcaa ataatttgct ggagctgcgt aatctggtac aggttgccga
    70561 gttgattgtt cgctgtgcga tgatgcgcaa ggagagtcgg gggttgcatt tcacgctgga
    70621 ttatccggaa ctactcacgc attccggtcc atcgatcctt tctcccggca atcattacat
    70681 aaacagataa aaagcctggg tcagcgccgt atacgcttcg gaatagttct ggtctggccc
    70741 acgaatgact aagcgatcgc taaagcattc tcccgcctgc ggggagaatg ccagtagcac
    70801 ccgatgcggc agtcgcgctt cgttttccgc cacatccgtc cgcaaacgta aatgccagcc
    70861 catacttaat gccagttcag taaaaccatt accaatctgc tctggcagca ccacgcagaa
    70921 aaatccctct tcagtaatgc actccgccgc acaagtcagc aacgatgggt gatcaagcgt
    70981 agtggtatag cgagcctgtt cccgttgaga tgtcgcgcac tctactccct gctgatagta
    71041 aggtgggttg ctgatgatta aatcgaagcg tactgtctgc tgtgtgatcc actgcagaat
    71101 atccgccgta tggacgttaa tccgctctgc ccacggggac tggttgatat tttcctgcgc
    71161 ttgcgcggca gcttcacttt ccagttcaac tgcatcaatc atcacgctgt catctgttcg
    71221 ctgcgccagc attaatgcca acaacccgct acccgcgccg atatcaaggc aacgttttac
    71281 cccagccaca ggtgcccatg cgcccagcaa aatgccatcc gttcccactt tcatcgcaca
    71341 acgatcgtga gcaacaaaaa actgtttaaa agtaaatcca ttacgacgaa gcacggatgt
    71401 agactgtgac atgaaaataa aaccttgcag gaaaaacggc gatagcaccg ggtgagaaca
    71461 atacccgaga agcgatatcc atacaaacag atgaagattg cagccgtaac gtctataatc
    71521 agcgccccac acagaggtag aacatgactg taacgacttt ttccgaactt gaactcgacg
    71581 aaagcctgct ggaagccctc caggataaag gtttcactcg cccgaccgcc attcaggctg
    71641 ccgccattcc gcctgcgctc gatggccgtg atgtactcgg ttctgcgccg acaggcaccg
    71701 gtaaaacggc ggcgtatctg ctgccagcgt tgcagcacct gctcgatttc ccacgtaaga
    71761 aatccggtcc gccgcgtatt ttgatcctca ccccaactcg cgagctggcg atgcaggtgg
    71821 ccgatcatgc ccgcgaactg gcgaaacata cgcatctgga tattgccacc atcaccggcg
    71881 gcgtggctta tatgaaccac gcagaagtgt tcagcgaaaa ccaggacatt gtggtcgcca
    71941 cgaccggacg tctgctgcaa tacataaaag aagagaactt cgattgccgc gcggttgaaa
    72001 cgctgatcct cgacgaagca gaccgtatgc tggatatggg cttcgcccag gatatcgaac
    72061 atattgctgg cgaaacgcgc tggcgtaaac agaccctgct cttttcggca acgctggaag
    72121 gcgatgcgat tcaggatttt gccgagcgtc tgctggaaga tccggtggaa gtttctgcca
    72181 atccgtccac ccgtgagcgc aaaaaaattc atcagtggta ttaccgcgcc gatgatcttg
    72241 agcataaaac cgcgttgctg gtgcatctgt taaaacagcc ggaagcgacc cgctcaattg
    72301 tgtttgtgcg taagcgtgag cgtgtgcatg agctggcaaa ctggctgcgc gaagcaggca
    72361 tcaacaactg ctatctcgaa ggtgagatgg tacaaggcaa gcgtaacgaa gcgattaaac
    72421 gcttgaccga aggccgcgta aacgtactgg ttgctaccga tgttgccgcg cgcggtatcg
    72481 acattcctga cgtcagccac gtctttaact tcgatatgcc gcgcagtggc gatacttatt
    72541 tgcaccgtat cggacgtacc gcgcgcgccg gtcgtaaagg caccgcgatt tcgctggtgg
    72601 aagcccatga ccatctgctg ctgggcaaag ttggtcgcta tattgaagag ccaattaaag
    72661 ctcgcgtaat tgacgagtta cgcccgaaaa cgcgtgcgcc aagcgagaag cagaccggca
    72721 agccatcgaa gaaagtactg gcaaaacgcg ccgagaagaa aaaagctaaa gagaaagaga
    72781 agccgcgggt gaaaaaacgc catcgcgaca ccaaaaatat tggtaagcgc cgtaaaccaa
    72841 gcggaacggg cgtgccaccg caaacgacag aagagtaatc tcaatgccgg gtttaagccc
    72901 ggcattgttt ttatcccggc aaaaaacact cttcaacaca ctgaataaaa gtgtgcatcg
    72961 ccgggctaac cgcttttccg gcatggtgag cgcacattgc cgtaatggtc tgcgactgtt
    73021 cgccaaaagg caattcaatt aattctccgc actccaactc ttttaccacc gcaaaacgcg
    73081 gcaggtaact gactccgata tttgccgcga cacaacgctt gatgctttcg atacttataa
    73141 gctcaatggt gttttccacc gtgatccgcc gctggcgcag cgtgctctca aatatctgcc
    73201 ggaagacaca ttgtggttcg ttgataataa agctacaggc gttatgtctt cccggctcag
    73261 taaaatcgac atctgcaatt tgcggtgaag ccaccagcgc cagcggttgt tcacccaact
    73321 ctcgtcggtt cagagcatca tcattcccta cgcgataaaa aaccccgaca tcggcctcat
    73381 cattcagtag cgcatcacgg atcacgtaac agttcagcga ctgcaacgat aaacgcactt
    73441 ttggagcgcg ttgccgaaaa cgctgcaaca cctgcggcat tcgataagag agcaacgttt
    73501 cgcctgaaat aacgcgtagt tccccgtccg gatccgactc ttttttagca gcctcacgaa
    73561 gcgtatccat cactcgggta agttcataga tgtgcggcag cagctttttt ccttcgcggg
    73621 taaggcacat tcgtcgacca attttctcaa acaattgtac cgagaactcc tgctcaagct
    73681 gctgaatatg aaaagtcacc gtcgattgtg tgcagcacaa tttttgcgaa gctcgcaaaa
    73741 aggaaccctc ttccaccacg gttttaagtg taataaaacg gcgcagatcc ataaccccaa
    73801 acctatcgaa aatatcgaat ttagaatgta aaaacattca tttttttaaa tgttccgtgt
    73861 cgggtactgt ctaccaaaac agaggagata acaagtgaca ccgacccttt taagtgcttt
    73921 ttggacttac accctgatta ccgccatgac gccaggaccg aacaatattc tcgcccttag
    73981 ctctgctagc tctgctacgt cgcatggatt tcgtcaaagt acccgtgtgc tggcagggat
    74041 gagtctggga tttttgattg tgatgttact gtgtgcgggc atttcatttt cactggcagt
    74101 gattgacccg gcagcggtac accttttgag ttgggcgggg gcggcatata ttgtctggct
    74161 ggcgtggaaa atcgccacca gcccaacaaa ggaagacgga cttcagacaa aaccaatcag
    74221 tttttgggcc agctttgctt tgcagtttgt gaacgtcaaa atcattttgt acggtgttac
    74281 ggcactgtcg acgtttgttc tgccgcaaac acaggcgcta agctgggtag ttggcgtcag
    74341 cgttttgctg gcgatgattg ggacgtttgg caatgtgtgc tgggcgctgg cggggcatct
    74401 gtttcagcga ttgtttcgcc agtatggtcg ccagttaaat atcgtgcttg ccctgttgct
    74461 ggtctattgc gcggtacgca ttttctatta acgaaaaagc ggaagaggtc gccctcttcc
    74521 gcttagtaac ttgctactta agccttacag gctttcagta aaggtacgag cgataacgtc
    74581 gcgctgctgt tccggagtca gagagttaaa gcgaactgca taaccggata cacggatggt
    74641 cagctgcgga tatttttccg gatgcttaac tgcatcttcc agagtttcac gacgcagaac
    74701 gttaacgttc aggtgttgac caccttcaac gcgaacttct ggtttcactt ctactggaac
    74761 ttcacggtat tcaatgtcac ccagtttgct tactgcaacc acttcgtctt ctgcaaaacc
    74821 tgctttcgca acgatgcaac gcgcttcgcc tttttcgctg tccagcaacc agaaagagtt
    74881 cagcagatcg tcgttagcgg ctttagtaat ctggatacct gtaatcatgt gatgcctccc
    74941 cggcaaaatt atttgatttg ttcagcctgt cgcggccaat tggtaaaacc attgttgctt
    75001 gagtgtatat atactcctca aacacccttg attctttgat ttaaatcaat aaaaaccaca
    75061 tatcaagtgt ggtcacaaat ggattttatt gttttacatc aacttatgca gatgtgaaat
    75121 tttaccaatt tacatttttt tgcatgtctt tttgtctata aaatgagcat gattttgttc
    75181 tgtagaaaga agcagttaag ctaggcggat taaagattcg caggagagcg agatggctaa
    75241 cgaattaacc tggcatgatg tgctggctga agagaagcag caaccctatt ttcttaatac
    75301 ccttcagacc gtcgccagcg agcggcagtc cggcgtcact atctatccac cacaaaaaga
    75361 tgtctttaac gcgttccgct ttacggagtt gggtgacgtt aaagtggtga ttctcggtca
    75421 ggatccttat cacggaccag gacaggcgca tggtctggca ttttccgttc gccccggcat
    75481 tgcgacacca ccgtcactgc tgaatatgta taaagagctg gaaaatacta ttcccggttt
    75541 cacccgccct aatcatggtt atcttgaaag ctgggcgcgt cagggtgttc tgctgctcaa
    75601 tactgtcttg acggttcgcg ctggtcaggc gcattcccac gccagcctcg gctgggagac
    75661 gtttactgat aaagtcatca gcctcataaa tcagcatcgc gaaggcgtgg tgtttttgtt
    75721 gtggggatca catgcgcaaa agaaaggggc gattatagat aagcaacgcc atcatgtact
    75781 gaaagcaccg catccgtcgc cgctttcggc gcatcgtgga ttctttggct gcaaccattt
    75841 tgtgctggca aatcagtggc tggaacaacg tggcgagacg ccgattgact ggatgccagt
    75901 attaccggca gagagtgagt aaatttgcgg gaaaatgccg gatggcagag ttgccacccg
    75961 gctgatttat caggctttat tctgacgcca ccattcgcca agaagaacgc cggttgcgac
    76021 agagatattc agcccggcaa cgttgccagt accatcaatc ttcacgcgca gatcgttcgg
    76081 atcgcgtgcg gcatccggta acccttcata ttcctgaccc agcaccagta ccattttcgc
    76141 tggcagactg gttttgaaca gcggtttacc ctgctcgctg gaagttgtca ctacggtgta
    76201 acccgcctga cggaaatcat ccagcacgtt aacaatgttg tcgccagtaa tcggctgaac
    76261 gtgctctgcg ccgccttctg cggtacggat agccgcccca gactccagca gtgctgcatc
    76321 ctgcaccaca acacctttca cgccgaagtg cgcacagcta cgcatcatgc cgcccaggtt
    76381 atgcgggtta gattcgtttt ccagggccag tacacaatcc tgcgcgcctg cctggcttac
    76441 ccactgctgc acggttgtac cgttacgctt tttgatcagg aagcaaacgc cgccgtgatg
    76501 ttccgtgcct gacgcttttg tcagttccgc ttcatccacc acatggtacg ctttgcggtt
    76561 tgccgccatc cagcgcaagg cttctttaaa acgtggcgtt acgctctgga taaaccaggc
    76621 gcgaacaatc gcttccggac ggctctggaa cagtgcctga caggcgtttt cgccgtagac
    76681 gcgggtttct tccgcacgct gacgacgcaa aacttccgga tcaataaaac ttttaccact
    76741 gatgccaccg tgatcggcct tttccggcgt ctcatcaccc ggcgcgcggg aaaccgtgcg
    76801 ccacggcgaa tcttcccact tgcggtcacg gtgctgactc tgtttgtcat cgcgggcggg
    76861 gcgacggcca ccgtcagcac gagattttcc tggacgcccg ccccctttcc cggtacgcgg
    76921 gttgtgggta cgtttatcag aatcatcatc actgcggaca tacatcactt tgaccttgcc
    76981 gcttttacct ttcatttcat cgttcatgct tttctccacc agcgctgcgc gaagcgcgca
    77041 gattacccga agtccgcgcg gttcgccatg atttcgttcc aaagcctgtg tctatcatac
    77101 ccattgaata aaacagattg ttgtctggaa caatgtcccc gataatatgt aacatattag
    77161 aaacataccg gcgtcgttgc cgataagtct ccttactcat cccgaggtta gttatgaata
    77221 ccgtttgtac ccattgtcag gccatcaatc gcattcccga cgatcggatc gaagatgcgg
    77281 caaaatgcgg acgctgcggt cacgacttgt ttgacggaga ggtgattaat gcgaccggtg
    77341 aaacgctcga caaattgctg aaagatgatc tacctgtggt gatcgacttc tgggcaccgt
    77401 ggtgcggccc ctgccgtaat ttcgcaccaa tttttgaaga tgtcgcgcaa gagcgtagcg
    77461 gtaaagtgcg ctttgtgaaa gtgaataccg aagctgaacg tgaattgagc agccgctttg
    77521 gtattcgcag tattccgacg atcatgattt tcaaaaatgg tcaggttgtc gacatgctta
    77581 atggcgcagt gccgaaagcg ccgttcgata gctggctgaa tgaatctctt taatcttacc
    77641 ggggcgcatc ttgtgccccg ttttctcctc tgcgacaatg gcgttttttc gacgctccct
    77701 tatgaccgaa aacgctgttc tccagttacg cgccgagcgt attgcgcgtg caacacgtcc
    77761 ttttcttgcc cgcggtaatc gcgttcgtcg ctgccaacgc tgtcttttgc cagagaaatt
    77821 atgtctctgt tcgacaatta ccccagcaca agcaaaaagt cgcttctgtt tgctgatgtt
    77881 cgacaccgag ccaatgaagc ccagtaatac cgggcgtctg attgctgata ttttgcctga
    77941 taccgttgcg tttcaatggt cgcgtaccga accctcgcaa gatttgctgg atctggtgca
    78001 aaacccggac tatcagccaa tggtggtctt tcccgcttcg tatgctgatg agcaacggga
    78061 agtgatcttc acaccacctg ccggtaagcc tccgctattt atcatgctcg atggtacctg
    78121 gccagaagct cgcaagatgt ttcgtaaaag tccgtatctg gataatcttc ccgtcatttc
    78181 cgtcgatctt tctcggcttt ctgcctatcg cctgcgtgaa gcccaggctg aaggccaata
    78241 ttgtactgcc gaggtagcca tcgcactgtt agatatggcc ggcgatacca gggcggcgac
    78301 agggttaggc gagcatttta cccgctttaa aacacgctat ctggcaggaa aaacgcaaca
    78361 tccgggaagc atcacagcag aacagttaga aagcgtttaa aatcattcgg tcacttctgc
    78421 gggagaccgg tatgagtcag cgaggactgg aagcactact gcgaccaaaa tcgattgcgg
    78481 taattggcgc atcgatgaaa cccaatcgcg caggttacct gatgatgcgt aacctgctgg
    78541 cgggaggctt taacggaccg gtactcccgg tgacgccagc ctggaaagcg gtgttgggtg
    78601 tgttggcctg gccggatatt gcccgcttgc cctttacacc cgaccttgcg gttttatgta
    78661 ccaatgccag ccgtaatctt gctcttctgg aagagctcgg cgagaaaggc tgtaaaacct
    78721 gcattattct ttccgccccg gcatcgcaac acgaagatct ccgcgcctgc gccctgcgcc
    78781 ataacatgcg cctgctagga ccaaacagtc tgggcttact ggctccctgg caaggtctga
    78841 atgccagctt ttcacctgtg ccgattaaac gcggcaagct ggcgtttatt tcgcaatcgg
    78901 ctgctgtctc caacaccatc ctcgactggg cgcaacagcg tgagatgggc ttttcctact
    78961 ttattgcgct cggcgacagc ctggatatcg acgttgatga attgctcgac tatttggcgc
    79021 gcgacagtaa aaccagcgcc atcctgctct acctcgaaca attaagtgat gcgagacgtt
    79081 ttgtttcggc ggcccgtagt gcctcgcgta ataaaccgat tctggtgatt aaaagcggac
    79141 gtagcccggc ggctcaacgt ctgttgaaca ccacggcagg aatggatccg gcgtgggatg
    79201 cggctattca gcgtgccggt ttgttgcggg tacaggatac ccacgaactg ttttcggcgg
    79261 tggaaaccct tagccatatg cgcccgctgc gtggcgatcg gctgatgatt atcagcaacg
    79321 gtgccgcgcc tgccgcgctg gcgctggatg ccttatggtc acgcaatggc aagctggcta
    79381 cgctcagtga agaaacctgc cagaaattgc gcgatacgct gccagaacat gtagcaatct
    79441 ctaacccgct cgatctgcgc gatgacgcca gcagtgagca ttatattaaa acgctggata
    79501 ttctgctcca cagtcaggat tttgatgcgc tgatggttat tcattcgccc agcgccgctg
    79561 ctcccgcaac agaaagcgca catgcattaa ttgaagcggt aaagcatcat ccccgcagca
    79621 agtatgtttc tctgctgacg aattggtgcg gcgagcactc ctcgcaagag gcacgacgtt
    79681 tattcagcga agccgggctg ccgacctacc gcaccccgga aggaaccatc accgctttta
    79741 tgcatatagt ggagtaccgg cgtaatcaga agcaactacg cgaaacgccg gcgttgccca
    79801 gcaatctgac ctctaatacc gcagaagcgc atcttctgtt gcaacaggcc attgtcgaag
    79861 gggcaacgtc gctcgatacc catgaagttc agcctatcct gcaagcgtac ggcatgaaca
    79921 cacttcccac ctggattgcc actgacagca ccgaggctgt gcatattgcc gaacagattg
    79981 gttatccggt ggcactaaaa ttacgctctc cggatattcc acataaatca gaagttcagg
    80041 gcgtcatgct ctacttgcgt acagccaatg aagtccagca agccgcaaat gctattttcg
    80101 atcgcgtaaa aatggcctgg ccgcaggcgc gtatccacgg cctgctggta caaagtatgg
    80161 ctaaccgtgc tggcgctcag gagttgcgag tagtggttga gcacgatccg gttttcggac
    80221 cgttgatcat gctgggcgaa ggcggtgtgg agtggcgtcc tgaagatcaa gccgtcgtcg
    80281 cactaccgcc gctgaacatg aacctggcac gttatctggt cattcagggg atcaaaagta
    80341 aaaagattcg tgcgcgtagt gcgctacgcc cgctggatgt ggcgggatta agccagttac
    80401 tggtgcaggt ttctaacttg attgtcgatt gtccggagat tcagaggctg gatattcatc
    80461 ctttgctggc ttctggcaat gaatttaccg cgcttgatgt cacgctggat atcgcgccgt
    80521 ttgaaggcga taacgagagt cgcctggctg tccgccctta tcctcatcag ctggaagaat
    80581 gggtagaatt gaaaaacggc gaacgttgct tgttccgccc gattttgccg gaagatgagc
    80641 cacagcttca gcaattcatt tcgcgagtca ccaaagaaga tctttattac cgctacttta
    80701 gcgagatcaa cgaatttacc catgaagatt tagccaacat gacgcagatc gactacgatc
    80761 gggaaatggc gtttgtagcg ctacgacgta ttgatcaaac ggaagagatc ctcggcgtca
    80821 cgcgtgcgat ctccgatcct gataacatcg atgccgaatt tgccgtgctg gttcgttcgg
    80881 atctcaaagg gttaggctta ggtcgacgct taatggaaaa gttgattacc tatacgcgag
    80941 atcacggact gcaacgtctg aatggtatta cgatgccaaa caatcgtggc atggtggcgc
    81001 tggcccgcaa gctcgggttt aacgttgata tccagctcga agaggggatc gttgggctta
    81061 cgctaaatct tgcccagcgc gaggaatcat gagtaaggta ctggaaatgt tgaccactta
    81121 atcgggactg gtgttattat tgcccgctta tgtcgtctgc attgcacaga ggacccttca
    81181 atgaacagag aagaaatgca ctgtgatgtt gtcaaaattt aagcgtaata aacatcaaca
    81241 acaccttgcc caactaccca agatttctca atcagttgat gatgtcgatt tcttttacgc
    81301 tcccgccgac ttccgggaga cgctgctgga aaaaatagcc agcgcgaagc agcgcatttg
    81361 cattgtcgcc ctgtatctcg aacaggatga cggtggcaaa ggcattctga acgcgttgta
    81421 tgaggctaaa aggcagcgtc cggaactgga tgtgcgggtg ctggtcgact ggcatcgtgc
    81481 acaacgtgga cgcattggcg ctgcggcatc taacactaac gctgactggt actgccgcat
    81541 ggcgcaggaa aatccgggcg tagatgttcc ggtttatggc gttccaatca atacccgtga
    81601 agcccttggt gttctgcact ttaaaggctt tatcatcgac gatagcgtac tttatagcgg
    81661 tgccagcctg aacgatgttt acctgcatca gcacgataaa tatcgctacg accgttatca
    81721 tctgatccgt aaccgtaaga tgtcagacat tatgtttgaa tgggttacac agaatattat
    81781 gaatggccgc ggcgttaatc gtctggatga tgttaatcgg ccaaaaagcc cggaaatcaa
    81841 gaacgatatt cgtctgttcc gccaggagct gcgtgatgcc gcttatcatt tccagggcga
    81901 tgccgacaac gatcagcttt ccgtaacgcc gctagtgggg ctggggaaat cgagtctgtt
    81961 gaacaagacc attttccatc ttatgccttg tgccgagcag aaactaacca tctgtacgcc
    82021 atacttcaac ctgccagcaa tccttgtgcg caatattatc cagttgctgc gcgaagggaa
    82081 aaaggtcgaa attattgttg gtgataaaac cgcgaatgac ttctacattc cggaagatga
    82141 acctttcaag ataattggcg cattgcctta tctctatgag atcaatctgc gtcgtttcct
    82201 gagccgtttg cagtattacg tcaatactga ccagctagtg gttcggttat ggaaagatga
    82261 cgacaacacc tatcacctaa aagggatgtg ggttgatgat aagtggatgt tgatcaccgg
    82321 taataacctg aacccgcgcg cctggcgtct ggatctggaa aacgccattt tgatccacga
    82381 tccacaactg gagcttgcgc cgcagcgaga gaaagaactg gagctgatcc gcgagcatac
    82441 caccatcgtt aagcactatc gcgatctgca aagtattgcc gattatccgg tgaaggttcg
    82501 taaactcatc cgccgtttgc gccgtatccg catcgaccga ttaattagcc gcatcctgta
    82561 atcacaaccc cgtcctgtac ggggtttgtt ttttggaggc cacgttttgc gtattctttt
    82621 tgtctgttcg ctattgttac tttctggatg cagccatatg gctaacgata gctggagcgg
    82681 gcaggataaa gctcaacact ttatcgcctc ggcgatgctt tccgccgccg gaaatgaata
    82741 ttcacagcat caggggatga gccgggatcg cagtgccatg tttggattga tgttctctgt
    82801 cagtctgggg gcgtcaaaag agctttggga tagccgccct gaggggagcg gctggagctg
    82861 gaaggatttt gcctgggata tcgccggtgc aagcaccggc tataccgtct ggcaactgac
    82921 ccgtcactaa agccgcatcc cttttccttt acggtgcaat gttagtgaaa ccagaaacgc
    82981 caccacggcc atcaaggtca cataccagaa gaaggctgtt tccattccta ttgatttcag
    83041 cgacaacgct acgtactccg ccgaaccacc aaatatagca ttggcgaccg catatgacag
    83101 accaacgcct aatgcgcgaa cctgtgccgg gaacatctca gccttcagta ttccactgat
    83161 tgaagtataa aaactcacta tcagcagggc gcacatcacc agaccaaaag cggtataagg
    83221 cgaggaaacg ttctgcaatg ctgagagaat aggaacggta aaaatggttg ccagagaacc
    83281 gaaacataac attgaggtac ggcgaccaat cttatccgac agcgcgccaa taattggttg
    83341 aataagcatg aatacaaaca atgcggcagt cataatgcca ctcgccacgt tggcatgcat
    83401 tcccgcagta tttaccagat acttctgcat ataagtggtg aaggtataga aacaaaggga
    83461 gcccgcagcg gtaaaaccga gaaccatgat gaatgcacgg cgattgcgcc ataatccttt
    83521 cagagatcca gcttctttta aagcgcgtgt ttcttgttgt gaagtttcat ctaactgacg
    83581 acgtaaccac aacgccacaa cagctaacac agcccctaac gcgaaaggaa tacgccatcc
    83641 ccactctctg agtacagcgt cttccatggt gtgttgtaaa accacgacaa ccagtagagc
    83701 tagcagttgc ccgccgatca acgtcacata ctgaaatgat gcgtaaaaac ctttacgccc
    83761 ttcaacggca acttcactca tataggtggc gctggtgcca tattctccgc caacggataa
    83821 tccctggaat aaacgcgcga gaagcaataa tgccggagcc caggtaccga ttgtttcgta
    83881 gcctggcaaa caggcgataa ccagtgagcc gaaacacatc atacacaccg ataacagcat
    83941 cgattttttg cgaccatgtt tatcggctat gcggccaaac agccaaccgc ctattgggcg
    84001 catcagaaat cctgcagcaa aaacacctgc tgtttgcagt agttgagtcg tcgtgttccc
    84061 ggaagggaag aagatgtggg cgaagtagag tgaacagaac gagtagacat agaaatcgaa
    84121 ccactcgacc agattacctg aagaggcccc aacaattgcc caaatgcggc gacgagtatc
    84181 actacttgtc agtttgctgt ctgccgttac agtactttca gccatgccat tatgtctcct
    84241 gccgtaatcc aatgcttttg tcggtcgctt ttgtttattt tttggtaaga aaaaatgtta
    84301 tacatttgtt gcatatcatt atgcaacctt aaccatgaat ttagttagca ggaaaatggt
    84361 tattaaggaa cttaaggata gatttctggt aaggaggaag tatggaagtg ggcaatttgg
    84421 ggaagacgta ttcgctgttg aatctggctt atgtgggagt ataagacgtg ccagcgtcgc
    84481 atccggcatt tattccgcac caacgcaaaa aagcccagtc ttccgactgg gccttt
//
LOCUS       DAZKHK010000005        74874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKHK010000005 DAZKHK010000000
VERSION     DAZKHK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.005"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            157..1002
                     /locus_tag="ACHOS5_000318"
     CDS             157..1002
                     /locus_tag="ACHOS5_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9775212.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPDNTDFTDITSLATFLLK
                     KQAEDLIQSTQFFNAEVLESILKLLANCDTIFFFAAGNSNPLAVYGAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLMVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(1032..3029)
                     /gene="tkt"
                     /locus_tag="ACHOS5_000319"
     CDS             complement(1032..3029)
                     /gene="tkt"
                     /locus_tag="ACHOS5_000319"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9775213.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAAWDATEKGAQVQQEWDALFADYAKQWPELAVEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(3080..4588)
                     /gene="ptsG"
                     /locus_tag="ACHOS5_000320"
     CDS             complement(3080..4588)
                     /gene="ptsG"
                     /locus_tag="ACHOS5_000320"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFP9775214.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            complement(4630..5187)
                     /gene="hxlB"
                     /locus_tag="ACHOS5_000321"
     CDS             complement(4630..5187)
                     /gene="hxlB"
                     /locus_tag="ACHOS5_000321"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFP9775215.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
     gene            complement(5527..7788)
                     /gene="metE"
                     /locus_tag="ACHOS5_000322"
     CDS             complement(5527..7788)
                     /gene="metE"
                     /locus_tag="ACHOS5_000322"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9775216.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPQRHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTIAALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            7906..8859
                     /gene="metR"
                     /locus_tag="ACHOS5_000323"
     CDS             7906..8859
                     /gene="metR"
                     /locus_tag="ACHOS5_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP9775217.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALESFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(8747..9646)
                     /gene="bioP"
                     /locus_tag="ACHOS5_000324"
     CDS             complement(8747..9646)
                     /gene="bioP"
                     /locus_tag="ACHOS5_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP9775218.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9722..10522)
                     /gene="yigL"
                     /locus_tag="ACHOS5_000325"
     CDS             complement(9722..10522)
                     /gene="yigL"
                     /locus_tag="ACHOS5_000325"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP9775219.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGRIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPEII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(10530..11552)
                     /gene="pldB"
                     /locus_tag="ACHOS5_000326"
     CDS             complement(10530..11552)
                     /gene="pldB"
                     /locus_tag="ACHOS5_000326"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP9775220.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            11663..12283
                     /gene="rhtB"
                     /locus_tag="ACHOS5_000327"
     CDS             11663..12283
                     /gene="rhtB"
                     /locus_tag="ACHOS5_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP9775221.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(12344..12964)
                     /gene="rhtC"
                     /locus_tag="ACHOS5_000328"
     CDS             complement(12344..12964)
                     /gene="rhtC"
                     /locus_tag="ACHOS5_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP9775222.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(13028..14857)
                     /gene="recQ"
                     /locus_tag="ACHOS5_000329"
     CDS             complement(13028..14857)
                     /gene="recQ"
                     /locus_tag="ACHOS5_000329"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9775223.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(14982..15851)
                     /gene="pldA"
                     /locus_tag="ACHOS5_000330"
     CDS             complement(14982..15851)
                     /gene="pldA"
                     /locus_tag="ACHOS5_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP9775224.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            16016..16483
                     /gene="yigI"
                     /locus_tag="ACHOS5_000331"
     CDS             16016..16483
                     /gene="yigI"
                     /locus_tag="ACHOS5_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP9775225.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLVF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            16535..17422
                     /gene="rarD"
                     /locus_tag="ACHOS5_000332"
     CDS             16535..17422
                     /gene="rarD"
                     /locus_tag="ACHOS5_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP9775226.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(17551..18501)
                     /gene="corA"
                     /locus_tag="ACHOS5_000333"
     CDS             complement(17551..18501)
                     /gene="corA"
                     /locus_tag="ACHOS5_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP9775227.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(18653..18709)
                     /gene="ysgD"
                     /locus_tag="ACHOS5_000334"
     CDS             complement(18653..18709)
                     /gene="ysgD"
                     /locus_tag="ACHOS5_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP9775228.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18965..19867
                     /locus_tag="ACHOS5_000335"
     CDS             18965..19867
                     /locus_tag="ACHOS5_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFP9775229.1"
                     /translation="MKKTFSALFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(19912..22074)
                     /gene="uvrD"
                     /locus_tag="ACHOS5_000336"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(19912..22074)
                     /gene="uvrD"
                     /locus_tag="ACHOS5_000336"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP9775230.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(22158..22874)
                     /gene="yigB"
                     /locus_tag="ACHOS5_000337"
     CDS             complement(22158..22874)
                     /gene="yigB"
                     /locus_tag="ACHOS5_000337"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP9775231.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGHYFEFVLRAGPHG
                     RSKPFSDMYLLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLASLI"
     gene            complement(22874..23770)
                     /gene="xerC"
                     /locus_tag="ACHOS5_000338"
     CDS             complement(22874..23770)
                     /gene="xerC"
                     /locus_tag="ACHOS5_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9775232.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(23767..24474)
                     /gene="yigA"
                     /locus_tag="ACHOS5_000339"
     CDS             complement(23767..24474)
                     /gene="yigA"
                     /locus_tag="ACHOS5_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFP9775233.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNYTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            complement(24471..25295)
                     /gene="dapF"
                     /locus_tag="ACHOS5_000340"
     CDS             complement(24471..25295)
                     /gene="dapF"
                     /locus_tag="ACHOS5_000340"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9775234.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPDLIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(25332..25535)
                     /gene="yifL"
                     /locus_tag="ACHOS5_000341"
     CDS             complement(25332..25535)
                     /gene="yifL"
                     /locus_tag="ACHOS5_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9775235.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            25724..27193
                     /locus_tag="ACHOS5_000342"
     CDS             25724..27193
                     /locus_tag="ACHOS5_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP9775236.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLEDWETVPPATNLMTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLLAPFGPWDTLARTRTKILINGEQLDTDPDTFSVVRWMPGSLLTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLLEKKVFWRQGPDCKQEELPGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPNHSGLHVFES
                     DGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVRLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            27190..28518
                     /locus_tag="ACHOS5_000343"
     CDS             27190..28518
                     /locus_tag="ACHOS5_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP9775237.1"
                     /translation="MNGQIITVALILLGHIFLTPAVQAIGFDYYNDHGVMSYGKGYGE
                     DEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGAHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDRQWSKWIDLPGIEPEQFHLITDNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDDSFNSGKQHAYFIRQLRSRKSVQIIPVNGPLTKNDRFAYDDRNVYTWTD
                     TEVRITPSHCPAKTRVREENVREIHNSDIIIPVTDESCRNAAADGQTLKP"
     gene            28566..28886
                     /gene="cyaY"
                     /locus_tag="ACHOS5_000344"
     CDS             28566..28886
                     /gene="cyaY"
                     /locus_tag="ACHOS5_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP9775238.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(28926..31472)
                     /gene="cyaA"
                     /locus_tag="ACHOS5_000345"
     CDS             complement(28926..31472)
                     /gene="cyaA"
                     /locus_tag="ACHOS5_000345"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP9775239.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGVVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            31859..32800
                     /gene="hemC"
                     /locus_tag="ACHOS5_000346"
     CDS             31859..32800
                     /gene="hemC"
                     /locus_tag="ACHOS5_000346"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP9775240.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNTYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            32797..33537
                     /gene="hemD"
                     /locus_tag="ACHOS5_000347"
     CDS             32797..33537
                     /gene="hemD"
                     /locus_tag="ACHOS5_000347"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP9775241.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSRKVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            33559..34752
                     /gene="hemX"
                     /locus_tag="ACHOS5_000348"
     CDS             33559..34752
                     /gene="hemX"
                     /locus_tag="ACHOS5_000348"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP9775242.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQADAPAA
                     APQGE"
     gene            34755..35951
                     /gene="hemY"
                     /locus_tag="ACHOS5_000349"
     CDS             34755..35951
                     /gene="hemY"
                     /locus_tag="ACHOS5_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP9775243.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNTPPQ"
     gene            36630..38285
                     /gene="aslA"
                     /locus_tag="ACHOS5_000350"
     CDS             36630..38285
                     /gene="aslA"
                     /locus_tag="ACHOS5_000350"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFP9775244.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPARSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(38387..39622)
                     /gene="aslB"
                     /locus_tag="ACHOS5_000351"
     CDS             complement(38387..39622)
                     /gene="aslB"
                     /locus_tag="ACHOS5_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9775245.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(39769..39845)
                     /locus_tag="ACHOS5_000352"
     tRNA            complement(39769..39845)
                     /locus_tag="ACHOS5_000352"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39809..39811),aa:Pro,seq:tgg)
     gene            complement(39888..39974)
                     /locus_tag="ACHOS5_000353"
     tRNA            complement(39888..39974)
                     /locus_tag="ACHOS5_000353"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39938..39940),aa:Leu,seq:cag)
     gene            complement(39995..40070)
                     /locus_tag="ACHOS5_000354"
     tRNA            complement(39995..40070)
                     /locus_tag="ACHOS5_000354"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40035..40037),aa:His,seq:gtg)
     gene            complement(40129..40205)
                     /locus_tag="ACHOS5_000355"
     tRNA            complement(40129..40205)
                     /locus_tag="ACHOS5_000355"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40169..40171),aa:Arg,seq:ccg)
     gene            complement(40308..41693)
                     /gene="yifK"
                     /locus_tag="ACHOS5_000356"
     CDS             complement(40308..41693)
                     /gene="yifK"
                     /locus_tag="ACHOS5_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9775246.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(41884..42624)
                     /gene="wecG"
                     /locus_tag="ACHOS5_000357"
     CDS             complement(41884..42624)
                     /gene="wecG"
                     /locus_tag="ACHOS5_000357"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP9775247.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(42627..43979)
                     /gene="wzyE"
                     /locus_tag="ACHOS5_000358"
     CDS             complement(42627..43979)
                     /gene="wzyE"
                     /locus_tag="ACHOS5_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP9775248.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMVIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(43976..45055)
                     /gene="rffT"
                     /locus_tag="ACHOS5_000359"
                     /gene_synonym="wecF"
     CDS             complement(43976..45055)
                     /gene="rffT"
                     /locus_tag="ACHOS5_000359"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP9775249.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAHEFMVAGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(45052..46302)
                     /gene="wzxE"
                     /locus_tag="ACHOS5_000360"
     CDS             complement(45052..46302)
                     /gene="wzxE"
                     /locus_tag="ACHOS5_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP9775250.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(46304..47434)
                     /gene="rffA"
                     /locus_tag="ACHOS5_000361"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(46304..47434)
                     /gene="rffA"
                     /locus_tag="ACHOS5_000361"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP9775251.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(47439..48113)
                     /gene="rffC"
                     /locus_tag="ACHOS5_000362"
                     /gene_synonym="wecD"
     CDS             complement(47439..48113)
                     /gene="rffC"
                     /locus_tag="ACHOS5_000362"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP9775252.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVCISPDAALLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(48091..48972)
                     /gene="rfbA"
                     /locus_tag="ACHOS5_000363"
     CDS             complement(48091..48972)
                     /gene="rfbA"
                     /locus_tag="ACHOS5_000363"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9775253.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(48991..50058)
                     /gene="rffG"
                     /locus_tag="ACHOS5_000364"
     CDS             complement(48991..50058)
                     /gene="rffG"
                     /locus_tag="ACHOS5_000364"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9775254.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWCKQVQDGSYQGERLGLKG"
     gene            complement(50058..51320)
                     /gene="wecC"
                     /locus_tag="ACHOS5_000365"
     CDS             complement(50058..51320)
                     /gene="wecC"
                     /locus_tag="ACHOS5_000365"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP9775255.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHELPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(51317..52447)
                     /gene="wecB"
                     /locus_tag="ACHOS5_000366"
     CDS             complement(51317..52447)
                     /gene="wecB"
                     /locus_tag="ACHOS5_000366"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9775256.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(52503..53549)
                     /gene="wzzE"
                     /locus_tag="ACHOS5_000367"
     CDS             complement(52503..53549)
                     /gene="wzzE"
                     /locus_tag="ACHOS5_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP9775257.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFMMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(53561..54664)
                     /gene="wecA"
                     /locus_tag="ACHOS5_000368"
     CDS             complement(53561..54664)
                     /gene="wecA"
                     /locus_tag="ACHOS5_000368"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP9775258.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(54904..56163)
                     /gene="rho"
                     /locus_tag="ACHOS5_000369"
     CDS             complement(54904..56163)
                     /gene="rho"
                     /locus_tag="ACHOS5_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9775259.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(56248..56349)
                     /gene="rhoL"
                     /locus_tag="ACHOS5_000370"
     CDS             complement(56248..56349)
                     /gene="rhoL"
                     /locus_tag="ACHOS5_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP9775260.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(56490..56819)
                     /gene="trxA"
                     /locus_tag="ACHOS5_000371"
     CDS             complement(56490..56819)
                     /gene="trxA"
                     /locus_tag="ACHOS5_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP9775261.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            56950..58215
                     /gene="rhlB"
                     /locus_tag="ACHOS5_000372"
     CDS             56950..58215
                     /gene="rhlB"
                     /locus_tag="ACHOS5_000372"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP9775262.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            58351..59835
                     /gene="gppA"
                     /locus_tag="ACHOS5_000373"
     CDS             58351..59835
                     /gene="gppA"
                     /locus_tag="ACHOS5_000373"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP9775263.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIVQESQWQSYVHWPLEVH"
     gene            complement(59882..61903)
                     /gene="rep"
                     /locus_tag="ACHOS5_000374"
     CDS             complement(59882..61903)
                     /gene="rep"
                     /locus_tag="ACHOS5_000374"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP9775264.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            61990..62271
                     /gene="ppiC"
                     /locus_tag="ACHOS5_000375"
     CDS             61990..62271
                     /gene="ppiC"
                     /locus_tag="ACHOS5_000375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP9775265.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(62317..63792)
                     /gene="ilvC"
                     /locus_tag="ACHOS5_000376"
     CDS             complement(62317..63792)
                     /gene="ilvC"
                     /locus_tag="ACHOS5_000376"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP9775266.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            63942..64832
                     /gene="ilvY"
                     /locus_tag="ACHOS5_000377"
     CDS             63942..64832
                     /gene="ilvY"
                     /locus_tag="ACHOS5_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP9775267.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(64829..66373)
                     /gene="ilvA"
                     /locus_tag="ACHOS5_000378"
     CDS             complement(64829..66373)
                     /gene="ilvA"
                     /locus_tag="ACHOS5_000378"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP9775268.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(66376..68226)
                     /gene="ilvD"
                     /locus_tag="ACHOS5_000379"
     CDS             complement(66376..68226)
                     /gene="ilvD"
                     /locus_tag="ACHOS5_000379"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP9775269.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDERSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(68291..69220)
                     /gene="ilvE"
                     /locus_tag="ACHOS5_000380"
     CDS             complement(68291..69220)
                     /gene="ilvE"
                     /locus_tag="ACHOS5_000380"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP9775270.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(69240..69503)
                     /gene="ilvM"
                     /locus_tag="ACHOS5_000381"
     CDS             complement(69240..69503)
                     /gene="ilvM"
                     /locus_tag="ACHOS5_000381"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP9775271.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(69500..71146)
                     /gene="ilvG"
                     /locus_tag="ACHOS5_000382"
     CDS             complement(69500..71146)
                     /gene="ilvG"
                     /locus_tag="ACHOS5_000382"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP9775272.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAA
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(71149..71199)
                     /gene="ilvX"
                     /locus_tag="ACHOS5_000383"
     CDS             complement(71149..71199)
                     /gene="ilvX"
                     /locus_tag="ACHOS5_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP9775273.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(71286..71384)
                     /gene="ilvL"
                     /locus_tag="ACHOS5_000384"
     CDS             complement(71286..71384)
                     /gene="ilvL"
                     /locus_tag="ACHOS5_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP9775274.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            71737..73257
                     /gene="yifB"
                     /locus_tag="ACHOS5_000385"
     CDS             71737..73257
                     /gene="yifB"
                     /locus_tag="ACHOS5_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP9775275.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(73282..73620)
                     /gene="maoP"
                     /locus_tag="ACHOS5_000386"
     CDS             complement(73282..73620)
                     /gene="maoP"
                     /locus_tag="ACHOS5_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP9775276.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            73739..74578
                     /gene="hdfR"
                     /locus_tag="ACHOS5_000387"
     CDS             73739..74578
                     /gene="hdfR"
                     /locus_tag="ACHOS5_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP9775277.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(74674..74749)
                     /locus_tag="ACHOS5_000388"
     tRNA            complement(74674..74749)
                     /locus_tag="ACHOS5_000388"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74714..74716),aa:Trp,seq:cca)
     gene            complement(74758..74834)
                     /locus_tag="ACHOS5_000389"
     tRNA            complement(74758..74834)
                     /locus_tag="ACHOS5_000389"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74798..74800),aa:Asp,seq:gtc)
BASE COUNT        18424 a        20283 c        18856 g        17311 t
ORIGIN      
        1 ccgtttaaat tatttttgtg cagttgacgt aattcatgaa aattaaccgg tctgttaatc
       61 attacgttca ctgcacacat tcattttttc acacctatcc acgcctgaaa actatgttat
      121 aatcagcact ttctgtcaga atgaaattta ctaattatga ataacataac cgtaaaaagt
      181 ctgattcaaa gcaattaccc tacactgcat caggcggaaa aaaaggtcgc tgactatatt
      241 cttagccacg cacatgaagt cgtgaactat agtgtcactg aactatcaga aaaaagccat
      301 gccagtgaag cgactatcgt acgcacctgt aagaaattag gttatcaggg ttattaccat
      361 ttaaaaattg cattagcaaa agaagtcatt aatcctgaca atagctatcc tgataatacc
      421 gattttaccg atataacgtc gctggccaca tttttattaa aaaaacaggc cgaagattta
      481 atacaatcaa cgcaattttt taatgccgaa gtgcttgagt ccattctcaa actacttgct
      541 aattgcgata ctattttctt ttttgctgcc ggaaattcaa accctctggc cgtttatggt
      601 gcttataaat tcagccaact cggattaaaa accgttgttc acgtcagccc ggaaatgcaa
      661 attaatgcgg catattctat ggggaaacgt gacctggcga ttggtatttc taactctggc
      721 agcaccaact taatggtcga tatctttaaa gtggtgaaag agcgcggtgc caaatccgtt
      781 tgtatcacta actatattaa atctccgtta tcaaaactct ccacccatca actcaatacc
      841 gcggtcagcg ataaaatctt ctttgaagcc ttcgactcca ctcgcgttcc agcaatgggc
      901 gttattgata tgctggtgct gttgtttatg tataaaaaca aggcgcatta cgagaaatac
      961 cgcacccgtg aagagtttct cggctcccgt ttcaaggggt aaaaggtcgt cctttcaccc
     1021 cttgtgcagg tttagctgtt cagtagctca cgcgcctgcg ccaggacatt ttccggcgta
     1081 aaaccaaagt gtttgaacaa cacattcgcc ggagcagact caccaaagct cgtcatgccg
     1141 atcactttac cgtccaggcc cacgtagcgt tcccagaatc cggcgatgct ggcttctacc
     1201 gccagacgtt tgcgtacagc ttttggcaat accgattctt tgtacgccgc atcctgatta
     1261 tcgaaacgct cggtacacgg catagaaacc acgcgaactt tacgcccttc ttccgttaac
     1321 gcttttgcgg cgctgaccac taactcaatc tctgaacccg cagaaatgag gatcatctca
     1381 ggctgaccgt cacaatcact cagtacataa ccaccacgcg caatctctgc caactgtgca
     1441 gcggtgcgcg gctgttgcgc cagaggttgg cgcgttaata ccaacgacgt tgggccatct
     1501 ttgcgctcaa tcgcctgttg ccaggccacc gccacttcca cctgatcgca accgcgccag
     1561 gtttccatat tcggcgtcag acgcagcgac gccagttgct caacgggctg atgtgtcggg
     1621 ccgtcttcgc ccaggccgat ggtatcgtgg gtataaacaa acaccgagcg gatcttcatc
     1681 agcgccgcca tgcgcagtgc attacgcgca tattccataa acatcaggaa agtaccgccg
     1741 taagggataa acccaccatg cagcgcaaga ccgttcatga tggccgacat accaaattca
     1801 cgcacgccat aggagatgta attccccgcc ggattttccc cggtgaaatc aaccgatttc
     1861 tgatggcgag tcaggttaga aggcgacaaa tccgccgagc cgcccatcag ttccggcagc
     1921 atatctgcga agtgattgag gcatttctgg ctaacctgac gtgttgccag cgccgccgga
     1981 tgggattgca gatcgtgcac atatttctgc atattctcga cccagccagc aggcaaatca
     2041 cccttcatgc gacgagtaaa ctccaccgcc agttccggcc actgcttcgc gtagtcggca
     2101 aacagtgcat cccattcctg ttgaacctgc gcgcctttct cggtcgcatc ccatgcggca
     2161 taaatttcac caggaatttc gaaaggggca tacggccatt gcagacgttc acgcaccagc
     2221 gccacttcat ctgcacccag cgccgagccg tggcaatcgt ggctgttggc tttatttggc
     2281 gaaccaaaac cgataatcgt tttgcagcac agcagactgg gcttatccgt gacgcttttc
     2341 gcttcacgca ccgcagcatc taccgcttct ggatcgtgac catcaacgcc ttcaatgaca
     2401 tgccagccgt aggcacgaaa acgggcagcc gtatcttcag caaaccagcc ttctacgtga
     2461 ccatcgatcg aaataccgtt atcgtcccag atagcaatca gattgccgag cttcagcgtt
     2521 cctgcaagac cacaggcctc gtgggagatc ccttccatca ggcagccatc acccaaaaat
     2581 agccatgtgt gatgatcaac aatattaaag cccggtttgt taaattctgc cgccagcgcc
     2641 ttctccgcaa tcgccatccc caccgcgtta gctacgccct gccccaacgg gccggtggtg
     2701 gtttcaacac ccggcgtata accatattct ggatgacccg gcgtgcggga gtggagctga
     2761 cgaaagtcgc gaatatcatc catcgacagg tcgtagcctg taagatgcag caaagcgtaa
     2821 atcaacatgg agccgtgacc gttcgactga acatagcggt cgcggttaaa ccactgcgga
     2881 ttttttgggt tatgacgcag gtgacgacgc cagaccactt cggcaatatc cgccatcccc
     2941 atcggcgcgc ccggatgacc ggagttcgct ttctggatcg catcaacact taacattcgg
     3001 atggcattag ccagtaattg tgaattcata cttcagctct ctctgtctgg cgggctgaaa
     3061 ccagcccgct aatctcgaat tagttagcac aggccactgt catttctgtc gggcgcggtc
     3121 tgctcattac ggttttgatt tcgtcgcgca ggatttgcga tttgccgcca aatatcacct
     3181 gtacaccgcc atcacccagt ttcactaaac ccgcagcccc caactccttc agccgttttt
     3241 cattcacttt cttatcgtct accagcgcca gcctcaggcg tgttgcgcag gcatccacat
     3301 cttcgatatt gtcgaaacca cctactgctt cgatgatttg gtatggacgt tcttcgcgcg
     3361 ttaatgtggt tgcaacggca atctcttcat cctcacgccc cggtgttttc agattcaact
     3421 tgcggatcag tacccgaaat acggtgtagt aaatcgctgc atagcccgcg cctgccagga
     3481 acaccagata ccacggcgtg cggtttggca taacgccatt cagtacgaaa tcaataagac
     3541 cgccagcaaa aggatgacca atgtgcacat tcaggatgtt catcagcaca aatgaagtgg
     3601 catataagaa gatgtgaatg atgtagagaa ccggagctgc aaacaaaaat gcatattcca
     3661 gcggctccgt aatcccggta acaaatgtgg tcagcgcacc ggagagtaaa aggcctttaa
     3721 tcagcgcctt acgttccggt tttgcttcct gatacatcgc caacgcggcg gcaggtaacg
     3781 caaacatcat gattgggaaa cgacctgtca taaacagccc cgccgtcaac ggaacaccgt
     3841 cgcgcaattg ggcaaagaac accggaatat cgcccgtcac cagttgcccg gatttggtca
     3901 tgtagtcgcc aaagttgtaa taaaacggca cgttccagat atgatgcagg ccaaaaggaa
     3961 tcagcgaacg ttcgccaacg gcgtaaataa aggccgcgaa gttcgcgtta tgcccactgg
     4021 tcaccagcgt tgaaagcgac gccagacctt tttgtaccgg tatccacagc cacggcaata
     4081 aatagccgac aataattgcc gccaccgccg taataatcgg tacaaagcgt tttccaccga
     4141 agaaaccaag aaactgaggc aattcaatat tgtggaagcg ggtatagagc agataaacaa
     4201 agatggcgac aatcaaaccg ccaaacactc ccgtttgtaa cgtcggtacc ccgaggatca
     4261 tcgcatagtc accgttactg gctgcaacgg actccgcggt aatgccaagt tgcgtaccca
     4321 tcgtggcatt catgatcatg tagctgataa tcgctgaaag tgaagaagaa ccatcacctg
     4381 tcaggccaat cacaataccg acacaaaaca gcaaaggaag gttggcgaaa atcaggccgc
     4441 cgcaattcgc cagcagctca atgttaaatg aaatcccgat ggctaaaatc aaacccgccg
     4501 ccggaagaat ggcgattggt aacatcagag cagaagaaaa tttctgcaac ttcttgaagt
     4561 taaacaagga tgttattttt ttgctcattt ttattctcct gtagggtatc gagcaaataa
     4621 cggcgacgac tattccagat tggcgtggcg cttaaacagt tcactttccg gcgcaccact
     4681 ttcagcagcc atcttcatca ctaatgcatc ggcaatcacc agcaaagctt gctcatacaa
     4741 cgacgccatc ggctgtgccg agcggaaaac agaatctttt tgatttttgg aaggggcatt
     4801 aatttgcacg atgacatcga caaactcggt gatagtggcg tgtcgattcg tggttaacac
     4861 cgccacagcg ccgccaagct gacgtgcttt tttcgccagt gccaccagtt gtgctgtctc
     4921 accggaagcg gaagaaacga taaacagatc gccaggccca aagttcggcg taatggtttc
     4981 cgtcaggaag taaacctctt ttcccatatg catcaggcgc atggtgaagg ctttcatgga
     5041 aaagccggca cgtcccaggc catagcagaa aacgcgctgg ctttgctgaa tagcgtcgat
     5101 caatttggtg cagtcatcct cacggataga gtccttaacg aaggttaatt cattgatgat
     5161 ctcagtgatg tacagctttg cgtccatttc acacctcatg aaataatttt tctcaaatac
     5221 aaaatctcat gaaattttat ttcattccaa tgcatcagta tgaaatgtga agctagtcac
     5281 aagaaagatc ataggttgat atgtttttca ttaaaaaaca tagagatatc aagtaatttt
     5341 taatatgtga tggagaggca gatagaaaaa atggcggaaa tgacgaaatt tatttcatga
     5401 gaagggaata aagaagattt tcttaaacag ggtatgaact gatatctgga atggtttacg
     5461 cagtatgctg ggaagacgcc gctgtaatga gaaaagaccg ggtggtatta ccacccggtt
     5521 tggattttac ccccgacgca aattctgcgc cgcctgcacc atgttcgcca gtgccgcgcg
     5581 ggtttctggc cagccgcgcg ttttcaggcc acagtccggg ttgacccaca gacgctctgc
     5641 cggaatgcgt tttgccgctt tcttcagcaa ggcttcaatc cattccacgc tcggtacgtt
     5701 tggcgagtga atgtcataga cgccaggacc gatttcattt ggataatcaa actcttcaaa
     5761 cgactccagc aactccatgt cggaacgcga ggtttcgatg gtgatgacgt ctgcgtccag
     5821 cgccgcaatc gaatccatga tgtcgttgaa ctcgcagtaa cacatgtgag tgtggatttg
     5881 tgtgtcatct ttcgccacgg cggcgttgat acggaaagcc tccacgcccc actggagata
     5941 ggcatcccag tcgctgcgac gcagtggtaa accttcgcgc aatgccggtt cgtcaatctg
     6001 aatgatgcca attccagcgg cttccaggtc cgcgacttca tcacgcagcg ccagcgcaat
     6061 ttgtttggcg atggtttcac ggctgacatc ttcacgcggg aacgaccagc agagaatagt
     6121 caccgggccg gtcaacatcc ctttcaccgg tttatcagtc agggattgcg catattttgc
     6181 ccactccacg gtaatcggtg ccgggcggct aacgtcacca ataacaatcg gtggcttcac
     6241 gcagcgggaa ccgtagctct gtacccaacc gttttgcgta aagacaaagc catccagatg
     6301 ctcgccaaag tattccacca tgtcattacg ctcggcctcg ccatgtacca gcacatccag
     6361 tcccaaacgt tcctgctcaa caatggcctg cttgatatgt tccgcaatgc ccgtgcggta
     6421 gttattggcg tcgagattac cctttttaaa atccagacgc agggtacgaa tctccgtggt
     6481 ttgcgggaag gaaccaatcg tggtggtcgg ccacgcgggc agtttaaaac gcgcacgctg
     6541 agcttcagca cgcacttcat agacattcgc acgctgactg tcctgagcgg tgatcgccgc
     6601 cagacgcttt tctactgccg gattatgtac acgagtagag tgacgacgcg cctgaatcgg
     6661 agcgctccac tctgccagag ctgccgtatc accactgttt agcgcatcgc gcagcaatgc
     6721 cagttcatga catttttgca gggcgaaggc aaaccagctt ttcacttctg catcaagacg
     6781 cgtttccacg ctcaggtcga tggggctgtg cagcaacgag caggaagatg ccacccacaa
     6841 atcgcgtttg ccgacaatgt ccttaatttg cgcatatttc tcggtaagat cggcgcgcca
     6901 gacgttacga ccattgataa gacccgcaga cagcagccag tcagaaggca gacgcttgtg
     6961 cagttcagca acgtcatctt taccatgtac aagatcgaca tgcagaccct gaacaggcag
     7021 cgcagcaatc gtgtcgaggt ttggcgttac gccttcaaaa taggtggtca gcagcagttt
     7081 cacctgtccc tggagcgcgt cgtaagcggg tttgtatgcg tccagccacg cctgcggcag
     7141 ttccagtacc aacgcgggtt catcaatctg tacccactcg atgccgcgtt tcgccagttc
     7201 tgccagcact tgctgataaa ccggcagaat gtcgttcagc aggctcaggc gatcaaactg
     7261 ttcacctttc actttaccca gccacaggta ggtaatcggc cccagcagca caggtttcac
     7321 cttgtggccc agcgccagcg cctcgtccac ttcctccagc agctgcgtcc aggtcagttt
     7381 gaactgttgg cctttaacga actccggcac catgtagtga tagttggtgt taaaccattt
     7441 ggtcatttcc gctgccgccg caggttcgcc agtcggtgca cgtccacgac caatacggaa
     7501 cagggtgtcg atatctaccg aaccatcgtt gttctgatga cgttgcggaa cattacccag
     7561 cagcagactg gtggtcagta catgatcgta ccaggcaaaa tcgcccaccg gcagcaggtc
     7621 gatacccgct tgcttttgtt gatcccagtg acgagcacgc aattcacgcc ctaccgccag
     7681 cagttcttca cgcgtggagt tccccgccca ataactctct tgcgcttttt tcagctcgcg
     7741 acgcaggcca acgcgaggga aaccgagggt gtgattaaga attgtcatta tatgcccctt
     7801 atggaatttt tagtatttag ccgtccagat gtttacacat ccataattgg cggttactgt
     7861 atattcctca agcgcaaatt attcatgccg aagtgaagga ctttcatgat cgaagtaaaa
     7921 cacctgaaaa cgctacaagc gttgcggaac tgcggctcac tcgcagccgc tgcggcgacg
     7981 ttgcatcaaa cgcaatccgc cctgtctcac cagtttagcg atctggaaca acgccttggc
     8041 ttccggctat ttgtgcgtaa aagccagccg cttcgcttta caccgcaggg agaaatcctg
     8101 ttgcaactgg caaatcaggt gctgccgcaa attagccagg cactgcaagc ctgcaatgaa
     8161 ccgcagcaga cgcgcctgcg cattgccata gagtgccata gctgtattca gtggctgacg
     8221 cccgcattag aaagtttcca taagaactgg ccgcaggtgg agatggattt taaatcgggc
     8281 gtaacgtttg atccgcagcc tgcgttgcaa cagggcgagc tggatctggt gatgacgtcc
     8341 gatattctgc cgcgcagtgg cctgcattat tcgccgatgt tcgactatga agtgcgtctg
     8401 gtgttagcac ctgaccatcc actggcggca aaaacgcgta ttacaccgga agatctcgcc
     8461 agcgaaacgc tgttgattta tccggtgcag cgtagccgac tggatgtctg gaggcatttt
     8521 cttcagccag caggcgtcag cccgtcactg aaaagcgtcg ataacacctt attgttgatt
     8581 cagatggttg ccgcacggat gggtattgcc gcactaccgc attgggtggt ggagagtttt
     8641 gaacgccagg gtctggtggt caccaaaacc ctgggcgaag gcttgtggag ccgactgtac
     8701 gccgccgtgc gcgatggcga gcagcgtcag ccgattacag aagcgtttat tcgttcagcg
     8761 cgcaatcacg cctgcgatca tctgccgttt gtgaagagcg cggagcgacc cacttacgat
     8821 gcacccacag tgaggccagg atcaccagcg cgcctgtaat aaacgttggc cagtgcggct
     8881 gttggtgcca gatagccagg tttaccagca gccctgccgg aacgtgcata ttattcataa
     8941 tgcccagcgt tccggcgtcc acctgcgtcg caccgtagtt ccacataaag tagccaatcc
     9001 cggaagccac cacgccaaga aacaccagaa tgccccattg caacgtggtt tgcggcattt
     9061 tctgcgcatt tcccagcaag aaccatgcaa tcactgccac caggaacgcg ccaagataga
     9121 accacgcaaa ggcgttatgc tgcggcatcg ggcgagtttc catcagacgt ttgtaaccca
     9181 ccatgccgat ggcaaaagtg atattggaga gttgcaccag caataagcca gtccagaaat
     9241 ggtcggtgac ctgatcatag cgaataatcc cggcaccaat caccgccaac aaggcgctaa
     9301 aagcatagcc ccagcgcaga cggcgcttac tcatgatgtc ataaatcagc gtgatgtaga
     9361 gcggcgtcag tacggtgaac agcaacagtt cggagaccgt caggtagaga taggcgcgga
     9421 aactcagcat atacatcaca ccgagctgca tcgcgcccac cagcatatac aggccaaccg
     9481 ttttcaggct attgccacgg gtgcgcagaa acggcagaaa aacgagtgcc gccagaccaa
     9541 cgcgcaccag caccgcaaaa tagctatcga cgtgccccgc aaggtactcg ccataaaagc
     9601 taaaggagaa ggcccacaga atcgtggtga tgataagtag cgccacaatg gaaaactctt
     9661 tgattaacga gtatccattg tagcgaagta tcaggttgac aactgaccaa ataaagaaca
     9721 attacgataa atagagctta cgcagataat gcggcactgc gtcttcggcg ttagtaccaa
     9781 tcacttccag ctctggatgc agatctttca gccgttgatg cgcgctgccc ataatgcagc
     9841 ctttccccgc catcgacagc atttcggcat cgttcattcc atcgccaaag gcaatacaat
     9901 cttgcaggct gtagcccagt ttcttcgcca ccgcttccag cgcatggcct tttgaaacgc
     9961 cgcccgccat cacttccagg caggttaagg tagagaaact gacattgacg cgatcgcccc
    10021 agcgagcgtt aatcgcctgc tccagtggca gaagtttttc atgggaatcg caggtgaaga
    10081 acactttgct gacgccttcc ggctccagca atcccggctc atacagcgca taattgaaca
    10141 ccgcttcttt gaaaaagcgc atctcttccg ggcgatggcg attcataaac cattcatcat
    10201 cgcgataaac gttagtaatg atctccgggt tgtcgttgac cacgccaaac aggtcactgg
    10261 caatgtcgcg atccaggtta tgagcaaaaa tcaggttgcc atccagatcg tgaacgcgcg
    10321 cgccattgga ggtaatcatg taagacttaa tctccagatt atcgcgaatt cgccccacat
    10381 caacgtggtg acgaccggtc gcaaacacaa agttgatgcc gcgcgcggtg agcagcttca
    10441 gagtttcttt ggcgtaaggg gataacgtat ggtcgggaga aagtaacgtg ccatctaaat
    10501 cagacgcaac aacctggtac ataagaaatt taaacctctg tagagcggtt tccgctgggt
    10561 gagttatgcc tgttgaaaaa atcaacgata gcgtggagcg cgactgagcg cattgcgtcc
    10621 ttttcaaaaa ggatctcatg gtacgcacct ttaattacca acggccgtcc tccttcgaca
    10681 ggatggcccg cagcggtgcg tagttcacaa aaacggtcgt gcatgcggtt atccaccacg
    10741 cgttcctctt cagcttgtaa gagaagcgtt ggcgtggcgt catcacccgc accggctaac
    10801 acctgttcgc cagccagaat actttcgcgt acccaatggt aggtcggccc accgacgcga
    10861 atcgttgggt catcagcata gaaacgtaag ttacgtcgat atcgctgtct gctgtgggtc
    10921 agtacgttga tagcaaacgg caacgcgcgc cagcgcccgg tgcctatcgc atagccatca
    10981 cggaaacgtg gatgcgcttc ggcccagtta aggatctgcc gtgccataaa tgacggcata
    11041 cgaatcacaa tcccaaacat tggcgcagtt agcgcaatgg cgtcacatac acctggatgg
    11101 cgttgcagaa ataatgtgga gatcgcaccg cccatcgaat gtgccagtat atagcgttta
    11161 cgccacggac cgggctgaac ctcctgttgc cagaatgccg ccagatcatc aacataatca
    11221 ttaaagcgat taacatgccc gagatgtgga tcggctaaca ggcgaccgga acgtccctgc
    11281 ccgcgatggt cgatgattaa aacatcaaac cccaaatgga acaggtcgta ggccagttcc
    11341 gcatatttta cgtagctctc aatacgcccc ggacagatga ctaccacccg gtcatggtgc
    11401 tgtgcgcgaa aacggacaaa gcgaaccgga atgtcatcca caccagtaaa ctctgcttca
    11461 tcacgctgac gccagaaatc agtcagcggt cccatggtaa aagcagcaaa cgcgttttct
    11521 cttgtttccc agtctttttg ctgctgaaac atcgggtaat ctgcctctta aaccacgtaa
    11581 aatcgttttt ttaagcgtgc ctgacacaac gctgcgacag tagcgtattg tggcacaaaa
    11641 atagacacac cgggagttca tcatgacctt agaatggtgg tttgcctacc tgctgacatc
    11701 gatcatttta agcctgtcgc caggctctgg cgcaatcaac accatgacca cctcgctcaa
    11761 ccacggttat cgcggcgcgg tggcgtctat tgctgggctt cagaccggac tggcgattca
    11821 tattgtgctg gttggcgtgg ggttggggac gctattttcc cgctcagtga ttgcgtttga
    11881 agtgttgaag tgggcaggcg cagcttactt gatttggctg ggaatacagc aatggcgcgc
    11941 cgctggcgca attgatctta aatcgctggc ctccactcaa tcgcgtcgac atttgttcca
    12001 gcgcgcagtt tttgtgaatc tcaccaatcc caaaagtatt gtgtttctgg cggcgctatt
    12061 tccgcaattc atcatgccgc agcagccaca actgatgcag tatatcgtgc tcggcgtcac
    12121 cactattgtg gtcgatatta ttgtgatgat cggttacgcc acccttgctc aacggattgc
    12181 tctatggatt aaaggaccaa agcagatgaa ggcgctgaat aagattttcg gctcgttatt
    12241 tatgctggtg ggagcgctgt tagcatcggc gaggcatgcg tgaaaataat gtcggatgcg
    12301 gcgtaaacgc cttatccgac ttactctgat gacgcgtctg gcattaacgc gaaataatca
    12361 aatgaatgcc aaatccggca aataacgccc cggcaaaacc atcaatccac ttcgccagac
    12421 gttgataacc acggcgcatt tgcggcagag caaacaggct ggcaacgacg gtaaaccacg
    12481 ccagtgtttc gacaataatc aacgcgaaaa tgccccagcg ttcggtggtg ccgacattat
    12541 caccgacaaa cagcgagaac accgaaccaa agtagataat cgctttaggg ttcgcgagat
    12601 tggtcagtaa gcccttaaga aaactgcgcc cgcttttcgc cagttcgact tgtggcgcag
    12661 gggcagaaac aacctctttt ttcagtgcac cacgtagcat ctggtaaccc atccagcaga
    12721 gatacaggcc accgcccacc ataatcagcg tatgcagcca ggccattttt tcgataatca
    12781 aatgtaggcc aagcaacgca atcccagccc aaaccattac gccgcaggta atgcccagca
    12841 cgcccatcat cgcttcttta cgggaacgac taacagcggt ctgagagaca aaaaagaaat
    12901 cgggaccggg gctcataagc gccacaatgt gcaccatggc gacggtgagg aataacatca
    12961 acatacatta gactcgcggg ggaaacgttt acggagtctt catcctggca cttttttatg
    13021 ctgctgacta ctcttcgtcg tcaccatcga catgcgcacg aatcagcgcc ataaacggtt
    13081 tgccaaagcg ttccagcttg cgcataccaa cgccgttaac gctgagcatt tcgctggcgg
    13141 tgatcggcat ctgctccgcc atctcaatca aggttgcgtc gttaaacacc acgtacggcg
    13201 ggacattgct ttcatcggca atcgatttac gcagtttgcg taatttggcg aacagtttgc
    13261 gatcatagtt gccgccgaac gatttctgca tcgctttcgg tttgagcgcc acgatacgcg
    13321 gcacggcaag ttgcaaagag gattcgccgc gcagcaccgg acgcgcagcc tctgtcagtt
    13381 gtagggcaga atgctgggca atattttgcg tcaccaggcc gaggtgaatc agctggcgga
    13441 tcacgctcac ccaatgttca tggcttttat cacggcccat gccatagact ttcagtttgt
    13501 catgaccata gtcgcggata cgctggttat tagcaccgcg aatcacttcc accacataac
    13561 ccatcccaaa ccgctgattc acacgaccaa tggtggaaag ggcaatctga gcatcggttg
    13621 aaccgtcgta ctgtttcggc ggatcgaggc agatatcgca gttcccgcac ggctcctgac
    13681 gcccttcgcc aaaatagttc agcaatacca aacgacgaca agtttgcgct tcggcaaacg
    13741 cgcccatcgc attgagttta tgacgttcga tatcctgcaa ctgcccctgc ggcttctctt
    13801 ccagacaacg gcgcagccac gccatatcag ccggatcgta aaacagcatc gcttccgcgg
    13861 gcaggccatc acgcccggcg cgtccagttt cctgataata ggattcgata ttgcgcggaa
    13921 tatcaaaatg caccacgaag cgaacgttag gcttgttgat gcccatgccg aacgccaccg
    13981 tcgccaccac aatttgcagg tcatcgcgct ggaatttttc ctgcacatca gcgcgaacat
    14041 tattttccag cccggcatga taggccgccg cgctaattcc cttgctttgc aggcgtgcag
    14101 cggtgtcttc tacttttgcg cggctgttgc agtagataat tcccgactta ccgcgttgtt
    14161 cctgtacgta gcgcatcaac tgatcgagcg gtttgaattt ctccatcagc atgtagcgaa
    14221 tattcggacg gtcaaaactg ctgatttgaa ttagcggatc gttcagcccc agcaggcgca
    14281 caatatcctg gcgcgtggtg tcgtcggcgg tggcagtcag cgccataaac gggagcgtcg
    14341 gaaaacgctg acgcagctga ccgagggcag cgtattccgg gcggaaatcg tgaccccatt
    14401 gggagataca gtgcgcttca tcaacggcta ataacaccgg attccagtgc gccagatgct
    14461 caagaaagtt atccagcatc aggcgttccg gcgcgatata aagcaggcgg atttgcccgg
    14521 tgcggcagcc tgtcattact tcaagctgct gctcgcgggt ttgcgtcgag ttaaggcacg
    14581 ccgccgccac gccgttggct tgcagttgat ctacctgatc tttcatcaac gaaatcagcg
    14641 gtgagacaac cacggtaagg ccgtttagca ataaggcagg gatttgatag caaagggatt
    14701 ttccgccacc agtgggcatg acgaccaggc aatcgcgacc ggaaagtacg gtgtcgataa
    14761 tctcttcctg gccggggcga aactgttggt agccaaaggt ttcttgtaaa acctgtttag
    14821 ccccggactc cagattcaac acttccgcct gcgccacatt caccccgatt gcctgaaata
    14881 aaaacaggcg ctattttcag cgcctgcgcg agaaactgca atgcttttag tctctgagag
    14941 ggtaatcctg gatgctggat tccagatttg ccaaccattc atcaaaacag gtcgtttagc
    15001 ataactccca caccaacgcg ggtctggttg aagttatagt cgatgagcga ttcgccgtaa
    15061 ccactgtaaa cctgagtata aaggcgcaca tgtttggtga tcgggtaact taagcctaac
    15121 tccgcgccgc cgtaaccggt gttccagttg tactgccctt tcgcgctcag taccgcatca
    15181 ccgaggtgat agccgatttt aagctggtag taacccatat atttggtgat gtccgggtta
    15241 tcgtccgtat tccccaccac ataccacggc ttcacctcta ccagccagtt accgttttct
    15301 gccatcaggc gggtataaag gcggttccag ctgcgggaag tcgggtctga acgcccgttt
    15361 gagtcgtggt tgtagcccat ttccacatcg cgcagcgtcc agccagcaaa gttgtaatcc
    15421 gtggcaaaac cgaggaacaa ttgcggttcg tagttagttt cacgaaacgg tgatgactca
    15481 tcgctattag aaagctgcca ccaggatttc tgtgtgtagg aggcgcccag tacagagttt
    15541 ggccccaaaa tcccacgcca cagcggaaac gccaggctca actggaactt tacttcatcc
    15601 ttacgcgcgt tttccgccca gtcgtaactg gcaatcgctt ctttattcag atcgctggtt
    15661 tgggtgtaga tgatgtagtt ggtgtcataa ggataaaggg tgaacggatt gtcatgctcc
    15721 tgcagcatat tggcgataat actgccacgc accgctggcg cgtcatgcac ctctttcacc
    15781 gttgcctctt gtgcatatac tgccataggc aacataaaca ccggcaacaa ccagccctgc
    15841 agagtccgca tcgctcgtgt tctccgtaac gaattattga taaggtgaat atttttctgc
    15901 gggctatttt acatacttaa ctccatagcc gttagttatc ccttcctaaa gtagaaacca
    15961 acaacaaaga agcataaaat caacatttca ttaaccaatg aagtgtaagg atgttatgtc
    16021 tgccgtactg accgctgaac aagccctcaa attagtgggt gagatgtttg tttatcacat
    16081 gccatttaac cgcgcattgg ggatggaact ggagcgttac gaaaaagagt tcgcacagct
    16141 ggtctttaaa aatcagccga tgatggtggg caactgggcg caaagcattt tacacggcgg
    16201 ggtcattgcg tcggcgctgg atgtcgccgc aggtctggtg tgcgtgggaa gtaccttaac
    16261 ccgccacgaa accatcagtg aagatgaact acgccagcgg ctatcgcgga tggggaccat
    16321 tgatcttcgc gtcgattatc tgcgcccagg cagaggcgag cgttttactg ctaccagtag
    16381 cctgttacgt gcaggcaata aagtcgccgt cgcccgcgtt gaattacaca atgaagaaca
    16441 gctttatatt gccagtgcca ccgccaccta tatggtaggt tgaagacata aaagcaggta
    16501 aactgctgtt accaattaaa aatgagtatt cccgatggat gcaaaacaaa cgcggcaggg
    16561 cgtattactc gctcttgccg cttattttat ttggggtatt gcgccagcgt acttcaaact
    16621 gatttactac gtgcccgccg atgagatcct gacgcatcgc gtgatctggt cgtttttctt
    16681 tatggtggtg ctgatgagca tttgccgcca gtggtcctat ttaaaaacgc tgattcagac
    16741 gccacagaaa atttttatgc tggcagtctc tgccgtgctg attggcggca actggctact
    16801 gtttatctgg gcggtgaaca atcaccatat gctggaagcg agccttggtt actttattaa
    16861 cccgctggtg aacattgtgc tggggatgat tttcctcggc gagcgattcc gccggatgca
    16921 atggctggcg gtgattctgg cgatatgtgg agtgctggtt cagttgtgga cttttggttc
    16981 actgccgatc atcgcgctgg ggctggcgtt tagttttgct ttctacggcc tggtgcgcaa
    17041 gaagattgct gttgaagcgc aaaccggcat gttaatcgaa actatgtggt tgctgcccgt
    17101 ggcggcgatt tacctgtttg ctattgccga cagctcaacc agtcatatgg ggcaaaaccc
    17161 gatgtcgctg aatttactgc tgatcgccgc cggtattgtc accaccgtgc cactgttgtg
    17221 ttttactgca gcggcgacac gcctgcgtct ctcaacgtta ggctttttcc agtacattgg
    17281 tccgacgctg atgttcctgc tggctgtgac gttttatggt gaaaaaccgg gtgccgataa
    17341 gatggtaact ttcgccttta tttgggtggc gctggcaatt tttgtgatgg atgcgattta
    17401 tacgcagcgt agaaaaagtt aagcattaac atgggtaggc cgtttaaaga gcctacccac
    17461 taacttatct atctttgtat gttatctgcg ataccctcgg ctaaaatgtc gctgtcagca
    17521 cactaactta ctcacagcga catcgtaaag ttacagccag ttcttccgct taaagtacag
    17581 atacggtgcc aggcccgcga ggatcataaa gataatcgca cctgggtagc cgaagctcca
    17641 cttcagttct ggcataaact caaagttcat cccgtagctg gaagccacca gcgtcggtgg
    17701 caggaacacg acggatacca ccgagaagat tttgatgatg cggttctgct cgatgttgat
    17761 aaaacccatc gccgcctgca tcaggaagtt caccttctgg aatagagatt cgttatgcgg
    17821 cagcagggat tcgatatctc gcaggatttc acgcgcctgc tccagttgcc cgcccggtaa
    17881 acgcgcctta cgcaccagga agttcagcgc acgctgggta tccatcagac acaggcgaac
    17941 tttccagccg atatcttcca gttccgccag cgtggagagc gcctcgtcgt actcatcgcc
    18001 ctgatgccct tccataatca cccggctcaa ctgctccaga tcgctgtaga tgttttcaat
    18061 ttcatccgcc aactgttcga ttttggtttc gaacagatcc aacagcagct cgtaggcgtt
    18121 accgtccacc attgactggc tacgggcgcg catacgatac agacggaatg caggcagttc
    18181 acgctcacgc agagtaaaca gacgaccatc acggatggta aatgccacag tagagttacc
    18241 ggcgtgatct tccgcatctt caaagaagaa gaaggagtga atatgcaggc cgtcatcgtc
    18301 ttcaaagaaa cgtgccgatg cttcgatgtc ttccagttcc gggcgggttg ccaggctctg
    18361 accaagttca gattgtacgc gcagtcgctc gtcgtcgtcc ggttcgacaa gatcaatcca
    18421 tactgcattt acaaggggtt gtgactcttc gacttccagc cgggtcagtc ggttattttc
    18481 cagttgaaat gcgctcagca tgaccgggac tcccaatgct taaaatatcg gacagttcgg
    18541 tgggcacaca gaaacaaatt gggtttcaga ccattaaaca gcctgactca gcgcgacggg
    18601 aaaaattgag gtcgctgaca accgctaagg ctatcagcaa aaagggatag ccttaggagt
    18661 tgatcctgga tgacaggata gtgagccagt atctactggg tgtgtccaag gcgaatgtcc
    18721 tcttagagtg atcgtgcgcg catgttacgc cagcaaaatt ttgccgtcaa cacgcaacgg
    18781 cgacacattt ttttcctgct tcctcctgtt tacgttcccc ggaacactta aattttgtgg
    18841 ttaaaatctg aatccatcag gcgtgctctg ggtaaacaaa agagagagcg ttaaactgat
    18901 aagttccaga tgctcttcgt ctggcacaac acaggacaac atcagataac ttaaggaaat
    18961 atagatgaaa aaaacgtttt cagcgttgtt tttattaacc tgtctggggt catcatccgc
    19021 ttatgcggat aacgctttga ttttacaaac ggattttagc ctgaaagatg gcgccgtctc
    19081 ggcgatgaaa ggggtcgctt tcggcgttga ccataatctt aaaatatttg atttaacaca
    19141 cgaaattcca ccgtataaca tctgggaagg cgcttaccgc ctgtatcaga ctgccagtta
    19201 ctggccgcaa ggttcggtgt ttgttagcgt ggtcgatcca ggtgttggta cagaccgtaa
    19261 atcggtagtg ctgaaaacca aaaatggtca gtacttcgtt tcaccagata acggtacgct
    19321 cacgttggtg gcggaatcgc tgggtattga gagcgtccgg caaatcgatg aaaaaaccaa
    19381 ccgtctgaaa ggttccgaaa agtcatatac cttccacggt cgtgacgtgt atgcctacac
    19441 cggtgcacgt ctggcttccg gggcgatcac gttcgagcag gtggggccag agcttcctgc
    19501 aaaagttgtc gaactttctt atcagaaagc caaagcaaca aaaggtgaag tgaagggcaa
    19561 tatcccgatt ctcgatattc agtacggcaa cgtctggagc aatatcagcg acgagttgct
    19621 gaatcaggca ggaatcaaac tcaacgacac gttgtgtgtg accatttccg aaggttccca
    19681 gcaaaaatac gcagggaaaa tgccgtatgt cgcaagcttc ggcgatgtgc cggaaggcca
    19741 gccgatggtg tatctgaaca gcttattgaa tgtttccgtg gcgctgaata tggataattt
    19801 cgcgcagaaa catcaggttg cgtctggtgc tgactggaat attgatgtga agaagtgcgc
    19861 taagtaaggt gctgatgcgg aatgcggtat ggcgcaccgc attccgcaac gtcacaccgt
    19921 ctccaaccgg gcgtaagccg ccaccagcca tttaataccc tggccctgga atgccacctg
    19981 caaacggcta tgctcaccgc tgccttccat attgacgatg gtgccttcac caaacttagc
    20041 gtggcgtacg cgctggccga gcttgtagcc gctgtcgttc tcgaccatcg gcgtccccat
    20101 ccgttgatgg ctgaccgggc ggcttaccgt agcgcgcagg cgcacctctt ccacacactc
    20161 ttccggcaac tcaccgataa agcgcgacgg gcgatggtaa acctctttgc catacagacg
    20221 gcgagtttcc gcgtaggtca gcgtcagttt ctgcatcgcg cgggttacgc caacgtaggc
    20281 cagacggcgt tcttcttcca gacgtccgcc ttcatccagc gacatctggc ttgggaacat
    20341 gccctcttcc ataccaacga taaacacctg cgggaactcc aggcctttcg ccgagtgtag
    20401 cgtcatcaac tgcaccgcgt cctgccaggt atccgcctgc ccctcgcccg cttccagcgc
    20461 cgcatgggag aggaatgcct gcagcggcat taaatcttcg tcttcttcgt tgtagctgaa
    20521 ctggcgcgtt gccgtcacca gttcctctaa gttttcgata cgcgtctggc ctttttcgcc
    20581 cttctcctgc tcgtacatgg tgcgcaggcc ggagtcttta attacccggt cagtctgtac
    20641 atgcagcggc atatcggcag tttcttgcgc taaggcgtcg attaattcca taaatcgctg
    20701 caaggcgctg gcagcacgtc cggcgagggc tttttcctgc aacagttcac gacatgcctg
    20761 ccagagtgtt aactggcgat cgcgcgatgt ctgacgtacc acgtccagcg tccggtcacc
    20821 aataccccgc gttggcgtat tcaccacacg ctcaaacgcc gcgtcgtcgt tgcggttggc
    20881 aatcaggcgc agatacgaga gcgcatcttt gatttcctgg cgttcgaaga agcgcatccc
    20941 gccgtaaata cggtacggca tactcgcctg taataaggcc tcttccagta cacgcgactg
    21001 agcgttgctg cggtagagaa tggcgcactc ggcaagcgcc ccgccgttgt cctgccaggt
    21061 tttgatgcgg ttaaccacaa aacgcgcttc gtcgagttcg ttaaaagcgc aataaaggga
    21121 aataggctca ccgtccgcgc catcggtcca caatttttta cccagacgcc cgttgttgtt
    21181 ttcaatcagg gcgttcgcgg cgctcagaat attgctggta gagcggtagt tctgctccag
    21241 acggatagtt tcagcaccgg ggaaatcatt gaggaaacgc tgaatattct ccacctgcgc
    21301 tccgcgccag ccgtagattg actgatcgtc atcgccaacg atcatcactt tgccggtatc
    21361 gcccgccagc aggcggatcc acgcatactg aatgttgttg gtatcctgga attcgtccac
    21421 caggatattg gtaaaccgtt cgcgatagtg ctgaaggata tgcggcttgt taagccacaa
    21481 ctcgtgagca cgtagcagca actcggcgaa gtccaccagg cccgcgcggt cacacgcttc
    21541 ctgatacgcc tgatacacct tctgccaggt ctgctccacc ggattaccgt agctttgaat
    21601 atgatgtgga cgcaggcctt catctttctg gctgttgatg taccacattg cctggcgcgg
    21661 cggccactgc ttctcgtcga ggttcatcgc cttgatcaaa cgcttaagca gacgtagctg
    21721 gtcttcactg tcgaggatct ggaaatcctg cggcaggttg gcgtccatat ggtgcgcacg
    21781 cagcaggcgg tgcgccagcc cgtggaaggt gccgacccac ataccgccct ggctagtgcc
    21841 catcagttgc ccgatacgat gacgcatctc cgccgccgct ttgttggtaa acgtcaccgc
    21901 cataatcgaa tacggcgagc agttttccac gctcatcagc caggcgatac gatgcaccag
    21961 tacgcgcgtc ttaccactgc ccgcgcccgc cagcaccaga aggttgctgc gtggcgcggc
    22021 caccgcttcg cgctgtttgt cattaaggct gtcgagcagg taagaaacgt ccattggcac
    22081 cgccgcgtaa aaataggttg ggcggagaag cgcaacgccc tccgccaaaa agctgggtat
    22141 atatacagat ttgctgatta tatcagcgag gccagagatg ccaaccggga aatttccaga
    22201 tgcggcagta aacggctgtc ccaggtttgc atcagatcgc cattttccgg tctgatccaa
    22261 caggcctgca ttccactacg gatcgcgccc cccacatcgg tggtgagatc gtccccgaca
    22321 tgtaagatct caccgatcgg cacgttaagt ttttccgccg ccagtaagta catgtcgctg
    22381 aacggttttg agcgcccgtg cgggccagcg cgcagcacaa actcaaaata atgccccaaa
    22441 ccaaacagtt ccggctgggc gttaccgttg gtgatcgcca ccagcggcca tttcttcgcc
    22501 agctgtttta aggtgtcgtg ggtttgctgc gggacgtcga tccggctgcg ccatttggca
    22561 aagttgatca ttgccgcgtg tgcgcctgca ctggcttctt cggcactcag cccggcgtcg
    22621 agcatcgctt gttcaatcga acgaaaacgc cagcgcgtca cgtcgtgata aatctcgggt
    22681 tccgcttccc gtaccgcctg gcgcaggcgt tgcagatctt cattctggaa gctgcgcagc
    22741 gccggatgat aattttgcac aaaggtaagc gcctctcgct cggtgcgcaa aatcaccgga
    22801 cggttatcgt aaagggtatc atccaggtca aaggtgagcg ccgagatgcg ccccaaaggc
    22861 cggtaaaaac gcattatttc ccccgtttgg cgcgtggatg cgccgcatcg tacaccgagg
    22921 caaggtgttg aaaatcaaga tgagtataga tttgcgtggt ggagaggttg gcatgaccca
    22981 gcagctcctg cacaccacga agatcgccgc tcgactccag catatgcgta gcgaacgagt
    23041 gacgtaattt atgcgggtga acgtgattat tcagcccttg tttgatgccc cattcggcaa
    23101 agcgtttctg cacattacgc gcggagatac gcttgcccag tttcgacaga aaaagcgcgt
    23161 cgtcttcgct accaaacagg tcgcgcaaat caagccagtg ctcaatccac gccacagcgt
    23221 tgcgaccaat cggcaggcgg cgctctttgc tgccttttcc catcacccac acttcaccag
    23281 actccaggtc gaggtgtttg atatccagcc ccaccagctc ggaaagacgc agacccgcgc
    23341 cgtacatcac ttccagcatt gcacggtcgc gtacagcgag gggatcgttg atatcaatat
    23401 ccagcagccg attcatatca tcgacgtcga tgtttttcgg cagatgacgc ggcgctttcg
    23461 gtgccgaaac acctttcgcc gggttggctt tgagttcgtt ctggctgacc agccagtcaa
    23521 aaaagctacg tagcgcagaa agccgtaacg ccagacttgc agctcccagc cctttacggc
    23581 gactgcgtac agcaaaatta cgcaccatcg tcacatcaca ttgctgccag ctttgcaggc
    23641 cattttcgct ggcaaaattg atgatcgcct caagctgtcg ctggtagtta agcagggtta
    23701 tcgggctaag ctggcgctcc acgctcagat aacgtaggta gcgttctaca tcggtgtgta
    23761 aatcggtcat acgcgttcaa tccaacgcgc cagaagctcc ggcaacatca gtgcaatttc
    23821 atgaagtaac tgcgttccct gcccttgttg atagtgactg gcatcgcgac tggtaaacag
    23881 cacaacaccc aaatcagcat cgcttcccag catcgacatc gccaccgatc ccaccgcttt
    23941 cgcttccggt agcaccacca gcagctccgg tccgttaagc ggtcccagat agtgctgttc
    24001 ctgccccaga cgctgaatac gcagcggttc gaaagactga cggcttaatg ccagatgagt
    24061 gtagttcgac ggcgcaccta agcgccagcg atccggaaac aggcgcagac tcgcacctgc
    24121 caggccgaga tcgcgcgccc agcggtgaaa gcgcatcagc atatcatcga gactactggc
    24181 ggcggtgaga ctgcgctgca ggtagagtag acgataaaac aggccttcgt tggcgatagc
    24241 ctgttccatc aacaacgcca tgttctcttc cagaacatga atatgattac gtgcgcgggc
    24301 catgtgccac tcgaccaacg aaacggtgcc gcgtaccgga tgcggcacac gtatcgcttc
    24361 tactgcgcgc gcattgcgga taaaaaactc aggattttta atcagataat cgacaaccgc
    24421 ccggtcatca agctccgtga gtgtttcctg cagttcttcc cctggttgct tcatagatga
    24481 ataaatccgt cgtagacatg taccgccggg ccagtcatat ataacgggtg acccggacct
    24541 ttccaggcga tatcaagacg accgccaggg agttccacgc gtacttcttc ggccagcaaa
    24601 ccttgctgaa tccctaccgc caccgccgca cacgcgccgc tgccgcaggc ctgggtttct
    24661 cctgccccac gctcataaac gcgtaaacga atatgctcgc gcttaaccac ttgcataaaa
    24721 ccgatattgg cgcgctccgg aaaacgctcg tggctttcca gaacaggacc aagcgtttct
    24781 accgccgcgg tatcgacatc atcgacctga atcacgcaat gcggatttcc catcgacacc
    24841 acgccgcata agattgtctg ctcggcggcg cgcataatat aggtcttttc cgctttgtta
    24901 gcgcgaaacg gcacggcgga aggttcgaag ttgggttcgc ccatatttac gcggaccaga
    24961 tcatcatcgg tgacggtcag aaccatccgc ccgttggcgg tgctgacgcg gatatcgcgc
    25021 ttattggtca gccctttcag gcgcacaaaa cgggcaaagc agcgcgcgcc gttgccgcac
    25081 tgcgccactt cactgccatc agcattgaaa atgcgatagt gaaaatccag ttcaggatca
    25141 tacggcggct caaccaccag caattggtca aaccctaccc ccaggtgccg atccgccagg
    25201 cgacgaatca gatccggtga aaaaaagaca ttctgcgtta ccgcgtcgac gaccataaaa
    25261 tcgttgccaa ggccatgcat tttcgagaac tgcatcattt actccaatca cgcgggtaca
    25321 gaaactgact tttagtaatt cacctgggat ggaccatcgc cagtggcgcg gtcgttttta
    25381 tccggcaccg tggattgcgt ttgcgtctct accggtttgg tcggcggcgg tgcgttttta
    25441 tctgcaggcg ggaaatagag cggacctttc agaccgcagc ccgtcaggct gaagagagta
    25501 agtaatacag tgagtgcctt aaacacgttt ttcattatgg attgcctgta aagttcatcg
    25561 ctttctgctt tctatcatcg caggagaagg cgtaaaagca agcgtttagc gactctttat
    25621 gaagtctgaa aagtgaggaa atgctgcgag tataacgctt actcgtggcg atttatgttt
    25681 tgctggtagt ttcgtgatcg gcacctcgcg ccaggatggc tttatgcact atcgcaatgt
    25741 ggtttgtttg ttgtcttgtt cgttgttttt atcgtcggca tggggatgtc ggcttgatga
    25801 gccggaacat aatatttacc agaaacaggg aaaaggcgtg gtgtacctgc gcccttatga
    25861 aaagaccaat ctttcgctac cgcagattaa ctacaagcgt ctgcgtctgt taccgaattt
    25921 gctaattgac ccaaccaaac tggaggattg ggaaacggtg cccccagcta ccaatctcat
    25981 gacagacgtt gtctacagcg gcgcaaacgc aactcttccc cattactctt actatagcga
    26041 tggtcgcgct attctctacg ctggcgagat tgtgcaaaac cctccaggca caccgccagt
    26101 cgatatctcg tcatttcagg catggggtga ttttgccgca gataagtaca gcctctatta
    26161 cgaaggcaaa cgcaccgata gcaaccagca actaaaccgc cgaacgttgc gtcaggtaga
    26221 atttaacccg caatggaaac cagactggct aggtttgatt ctccgtgaca agcactatct
    26281 ttacgcaaat ggtcagcgcc ttgatgatcc cgacaccttt acggtactgg ctcaaaagtc
    26341 atgggatcag cgcggtaaat tctctacagc atttaatccc tgccttcttg ccccatttgg
    26401 cccctgggat accctggctc gtacaagaac caaaatcctg atcaacggcg aacagcttga
    26461 taccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
    26521 tgataaaaac gggttacagc gtaaagtcct cgacaaggaa aatctggcgt gggatgagga
    26581 tctaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtcaggg
    26641 gcctgactgt aaacaggaag aattaccggg actcgatccg gaacagtttc accccatcag
    26701 tgatgctgtc gcccagtatc aggactcgct ttataccatc atcgaaacag agtcaggtga
    26761 ccgcaagttg gagatcgtga aactcgatga ccccaatctt attatcaaca aacgtttcaa
    26821 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtggccaa accattccgg
    26881 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgct ctccggatga
    26941 acgcgaagca catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
    27001 ctacgtttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
    27061 agttcgctta atctggaagg aacaacattc gggatatggc tacggggtaa attacaaaac
    27121 gggatatctg gacggaaaaa ttaccgatga cggcgaattt attcctgccc cgcgcaatga
    27181 ggcgacaaaa tgaatggtca aataatcact gttgcactca tcctgttggg gcacatattt
    27241 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
    27301 tacggtaaag gttatgggga ggatgagaaa atcattgccc aatttccgaa gatgaacaga
    27361 gccgatttgc gccttgtgac aaatatatcc ggggagcggg agttggtgga aggctatata
    27421 cccaccgatg aaataagcat aaaaaatgag gaataccatt gggtgacaga tggccgcgtt
    27481 attctctggc gtggcaaaat cgttagcaat ccaccgggaa cacccactgt cgatattgcc
    27541 agctttcagg ctatgggccg cttcgcggtc gataaatata gcctctattt tgacggacag
    27601 cgtaccgaaa gtaatagcgg cgcgtcacgt gttgatctgg cgacactaaa agctatcgaa
    27661 ggtaactcta ccacgctggt ggatagtaaa aatctctact tgtccggtcg acgtcagggg
    27721 agcagcagta atgttactgt attagaaaaa agatggtggg gtattaatcc acgtcttatg
    27781 agtgtaaaca gaaatttgta ttccaatgat ctgcttatcc gcagtgggca gaatatttat
    27841 ttaaatggcg ctcaccttac ggcgaatgca gactcatttg agataattcg ctggatacct
    27901 cattcactgc tggtttttcg cgacaataag ggtctgcatc gttatccctt tggtcaatta
    27961 tctggcaaag cgatacccgt agatgatgac gtctcttttg aagtagggga aagtcgcgtt
    28021 cgctggcgta aacagctcac gcccgaccgt cagtggagca agtggataga cctaccgggt
    28081 attgaacctg aacaatttca tctgattact gacaatattg cgcagtataa agatcgactg
    28141 tatgtaacaa aattatcgac ttttggtgaa gaccagcttg agataatccc gctggatacg
    28201 ccagacctgg tcattgatga ctcatttaat agcggcaaac aacatgctta ctttatccgc
    28261 caattacggt cacgcaagag cgtgcaaatt attccagtta acggtccgct aactaaaaac
    28321 gatcgcttcg cttatgacga tcgcaatgtt tatacctgga ctgatacaga ggtaaggatt
    28381 acgccctccc actgcccggc gaaaacacgc gtcagagaag agaatgtccg tgaaattcat
    28441 aacagcgaca ttattattcc ggtgacggat gaatcatgcc ggaacgcagc agcagacggg
    28501 caaactttga agccctgacc ccatgccgct atactgccgc catcacttaa aaacaggata
    28561 caacaatgaa cgacagtgaa tttcatcgcc tggctgatca actgtggctg accattgaag
    28621 aacatctgga cgactgggat ggcgacagcg atatcgactg tgaaatcaac ggcggtgtac
    28681 taaccattac ctttgagaac ggcagcaaaa tcattatcaa ccgccaggaa ccactgcacc
    28741 aggtgtggct ggcaaccaaa cagggtggct accattttga tctgaaaggc gacgagtgga
    28801 tttgcgatcg tagcggtgaa accttctggg atttgctgga acaggcggcg acgcagcagg
    28861 cgggtgaaac agttagtttc cgctaagatt gcatgccgga taagcctcgc tttccggcac
    28921 gttcatcacg aaaaatactg ctgcaacagc ggcgcgtcat gctcctgatt ggcaggtggc
    28981 atgttaccga tagatttggt gcgaaacgga atcacctgtt cacgaccatc aaccttcaca
    29041 atctgataga actgcggcag gttgaagttg atgaagcttg agccgtaggt gaaacggtcg
    29101 tgcgaagacg agtagaagcg actgacgtca cgcaccagtt cctctttgct gccttcgcag
    29161 tggtgataca cctcaacccg gttgctttcg tcgagaatgt agatattaaa gccattctcg
    29221 tcttgcgttt cttcgaagaa aaactggatg atcccttcgc tggcaaagcc gtccaccacc
    29281 gccggtaatt tgacgtgatt ggtttcaacc tgcactgaca ggccgtgcag tttgttatgc
    29341 gaaatagcgc cataaaactc gatggcgttt tccagtttct gtaccgagac attcagccgt
    29401 tcgaagaaca agccccaggt ttgaccggag acgcgcagcg ccttgaagcg cccggtttcc
    29461 tggcgggtgc tggaaagacg cagttcaata cactcagaaa ccagttgctg cacgcgagta
    29521 cggattaagc cgcgcagatg ctggctataa cagaagactt ccacgctatc tggcggtgcg
    29581 gcgtcctgat gcattttgcc gagaatagtt ttcagggctt cgatcatcga ttgctcgccg
    29641 ttgaagtgca gcgtacgcac ttcgttccac gagttgcgat acagcaggtc aacgctacct
    29701 accaggcaat tttgattctc gccaaagctg aagacatcca gcttacggaa atcaaaatgc
    29761 accacctgat tgcggaacgc agctgtcggg tcatattcca ggttaacgat aatcgccaga
    29821 tggcggatct cacacgggct gtagagcgcc ttcggtgtcg gtgcaggtaa gcgcagcggg
    29881 aaatggtgcg acacgtcggc gaccatctcc tgcaacttcg gcaagtcgac aacgccgttg
    29941 cctttaatgt acaaacgggt gcgcgaggtc agcaggccgt taaaccatgc ccacgccacc
    30001 agtttattta ggtaacggtt atattccagc ggctgatggc tgatgattga ctcaatattt
    30061 ggcgcgcggt tatacagata ccaacctgaa cggttggcgc ggcccggcgg tacataaata
    30121 aaggtcagat tcggttccga gagatcgggc gaaatctgcg ggtttaccag cgtcacttta
    30181 cccggtaatg cttcaaacgc ggcgtacagt ttacgcgtca gcacgccgat atcctgcggg
    30241 ctggcggaga cgctaaggtt attgcgacgc gcaaagcgga tcagattacg gtagctctgc
    30301 atcattgcgt cgagcagctc gttgtgcgcc tcacgcacct gatctatctt ccagttagcg
    30361 cggttatcga gcatcgccag acgagcatcg tcccattccc actctttcac caactggctt
    30421 aacacttcac gacgccagcc tacgcaggcg cgttcacggc tgagtttttc gcaaactttt
    30481 aaatagaagc atcggcgaac taaatccaga cgagtaaaat cttcgatcgc cgtcagatat
    30541 tcagtgacgc gctccagcat catgcaatac gggtcgagac caaacgacac tatttcgccg
    30601 tcgtgcaaac gctgtttgat atctttcgcc agcagacgtg ggttcgggta ttcccaggaa
    30661 taggcttcca gcagcagtgt tttcagcacc gctttgtatg gggaatcgat actcttgtag
    30721 agctgccaaa ggctggcacc aaagtactct tcagcagaaa gcgagcttaa gccaccaaga
    30781 tccagccatt catttggcgt cagcacgccc tgcgcgtaaa gcgtcatcac atagtcgtcg
    30841 taatgctctt cttcgtcgca cggcaccata ttccacagaa tacgcttacc ggcgagacgc
    30901 acggcggtac gataaaattc gtcaagcagc agtatatgct gggtggagcc acaatcttcg
    30961 ccccccaggc tgccgctttc attatgacgg aagcggtttt catcaatcag gaagaagctg
    31021 acttccacac ccagcgaggc ggcccagctt tccagcagac tacatttacg ttgtagcaac
    31081 tggcgctctt cgctatcgag ccaggattga tgacagaccc agatatccag gtcagaggaa
    31141 caactttgcc ctacggacga ggtgctgccc atggtgtata caccagtaat tggaagctca
    31201 cctttcggtg ggtcctgtac tgacattcca cgatacagtt caagctcgtt caggtagtgg
    31261 cgttgagttt catcaggcgt gtaaaggcaa atgcctttgg gaacgttacc atcaaggtaa
    31321 cccggcatta gcggatggtg atagtgcaac aatgtcggca gtagactgta gacctgttgg
    31381 aatgcaggcc ccatagcagc aagcgcgcga tccacacgca attgatttat ggcatccagt
    31441 ctctgtttca gagtctcaat atagaggtac aagacgtatc gcctgatttg ctacccgtca
    31501 tgactgtgat tccgccaaca tcaacggtaa cacgcggcat tcgggatatt tcgtatgtca
    31561 aaggtaaccg ttaccacttt tcgcgcctgg tttttttagt ttcacgacga aaaaatggtc
    31621 taaaacgtga tcaatttaac accttgctga ttgaccgtaa agaaagatgc gctacataca
    31681 agtgtagcac cgtttattct ctgtaaattc cttattacga aggctcaaag cgcctgtcag
    31741 gatccactgc cagacctcat tttacggttt gcgcaggcgt ctacgtttca ccacaacact
    31801 gacatcgctc tggcaaggat gttaggatgg accacggatg ataatgacgg taacaagcat
    31861 gttagacaat gttttaagaa ttgccacacg ccaaagccca cttgcactct ggcaggcaca
    31921 ctatgtcaaa gacaagttga tggcgagcca tccgggcctg gtcgttgaac tggtaccgat
    31981 ggtgacgcgc ggcgatgtga ttcttgatac gccgctggcg aaagtaggcg ggaaaggctt
    32041 atttgtgaaa gagctggaag tcgcgctcct ggaaaatcgc gccgatattg ccgttcattc
    32101 aatgaaagat gtgcccgttg aattcccgca aggtctgggg ctggtcacca tttgcgagcg
    32161 cgaagatccc cgcgatgcct ttgtatccaa cacctatgac agtctggatg cgctaccggc
    32221 aggcagtatc gtcgggacgt ccagtttacg tcgccagtgc caactggctg aacgccgccc
    32281 ggatctgatt atccgctccc tgcgcggcaa cgtcggcact cgtctgagca aactggataa
    32341 cggcgaatat gatgccatca ttcttgccgt agccggacta aaacgtttag gtctggagtc
    32401 ccgcattcgc gccgcgttgc cacccgagat ttctcttccg gcggtagggc aaggtgcggt
    32461 gggcattgaa tgccgccttg atgatgcacg cactcgcgag ctgctcgccg cgctgaatca
    32521 ccacgaaact gcactgcgcg ttaccgccga acgcgccatg aatacccgtc tcgaaggcgg
    32581 atgtcaggtg ccaattggta gctacgccga gcttattgat ggcgaaatct ggctgcgtgc
    32641 gttggtcggc gcgccggacg gttcgcagat tattcgcggt gaacgccgcg gtgcgccgca
    32701 agatgccgaa caaatgggga tttcgctggc agaagagcta ctgaataacg gcgcgcgcga
    32761 gatcctcgct gaagtctata acggagacgc cccggcatga gtatcctggt cacccgcccg
    32821 tctcccgcag gagaagagtt agtgagccgt ctgcgcacac tggggcaggt ggcctggcat
    32881 tttccgctga ttgagttttc tccgggtcga caattaccgc aacttgctga tcaactggca
    32941 gcgctgggag agagcgatct gttgtttgcc ctctcgcaac acgcggttgc ttttgcccaa
    33001 tcacagctgc atcagcaaga tcgtaaatgg ccccgactac ctacttattt cgccattgga
    33061 cgcaccaccg cgctggcact acataccgta agcggacaga agattctcta cccgcaggat
    33121 cgggaaatca gcgaagtctt gctacaatta cctgaattac aaaatattgc gggcaaacgt
    33181 gcgctgatat tacgtggcaa tggcggtcgt gagctaattg gggattccct gacggcgcgc
    33241 ggtgctgagg tcactttttg tgaatgttat caacgatgcg caatccatta cgatggtgca
    33301 gaagaagcga tgcgctggca atcccgcaag gtgacgacgg tcgttgttac cagcggtgaa
    33361 atgttgcagc aactctggtc gctgatccca caatggtatc gtgagcactg gttactacgc
    33421 tgtcggctat tagtcgtcag tgagcgtttg gcgaaactcg cccgggaact gggctggcaa
    33481 gacattaagg tcgccgataa cgctgacaac gatgcgcttt tacgggcatt acaataactc
    33541 tcataacagg aagccataat gacggaacaa gaaaaaacct ccgccgtggt tgaagagacc
    33601 agggaggccg tggacaccac gtcacaacct gtcgcaacag aaaaaaagag taagaacaat
    33661 accgcattga ttctcagcgc ggtggccatc gctattgctc tggcggcggg cgtcggtttg
    33721 tatggctggg gtaaacaaca ggccgtcaat cagactgcca ccagcgatgc cctggctaac
    33781 caacttacgg cactgcaaaa agcccaggag agccaaaaag ccgagctgga aggcattatc
    33841 aagcaacaag ctgtacagct tgagcaggcg aatcgtcagc aagaaacgct ggcaaaacag
    33901 ctggatgaag tccaacaaaa ggtcgccacc atttccggca gcgatgctaa aacctggctg
    33961 ctggcccagg ccgattttct ggtgaaactc gccggacgga agctgtggag cgatcaggac
    34021 gtcacgaccg ctgcagcgtt gctgaaaagt gcagacgcca gcctggcgga tatgaatgac
    34081 ccgagtctga ttaccgttcg ccgggcaatt accgatgata tcgccagcct ttctgcggta
    34141 tcgcaggtgg attatgacgg tatcatcctt aagcttaatc agctttcaaa tcaggtagat
    34201 aatctgcgtc tggccgataa tgacagcgat ggttcgccga tggattccga cggtgaagag
    34261 ctttccagtt ccatcagcga atggcgtatc aatctgcaaa aaagctggca gaactttatg
    34321 gacaacttca ttacgattcg ccgtcgtgat gacaccgccg taccgctgtt agcgccaaat
    34381 caggatatct atctgcgcga aaatattcgc tctcgcctgc tggtcgcagc acaagctgta
    34441 ccgcgtcacc aggaagagac ttatcgccag gcgctggaga acgtctccac ctgggtacgt
    34501 gcttactacg atactgatga tgccaccacc aaagcgttcc tcgacgaggt ggaccagtta
    34561 agccagcaaa atatctcgat ggatcttccg gaaaccctgc aaagccaggc gatgctggaa
    34621 aaattgatgc agacccgcgt gcgtaacctg ctggcacaac cggcagcagg gacaacggaa
    34681 gctaaacctg cacctgcacc tgcacctgca ccgcaagctg atgctccggc agccgcgccg
    34741 caaggagaat aaccatgcta aaagtgttat tgctctttgt gttgctgatt gcggggatcg
    34801 tggttggccc gatgattgcc ggccatcagg gttacgtgct gatccagacc gacaactaca
    34861 atattgaaac tagcgtcacg ggcctcgcga ttattttgat tctggcgatg gtagtgctgt
    34921 ttgccattga gtggctactg cggcgaatct tccgcacagg cgcgcacacc cgtgggtggt
    34981 ttgtcggacg taagcgtcgc cgtgcacgta agcagaccga acaggcgctg ctgaaactgg
    35041 cggaaggcga ttatcagcaa gttgaaaagc tgatggcgaa aaatgccgat cacgctgaac
    35101 aaccggtggt gaactatcta ctggcagccg aagccgcgca acaacgtggt gatgaagcac
    35161 gcgccaacca acatctggaa cgcgcagcgg agctggccgg caatgacacc attccggtag
    35221 aaatcacccg tgtacgtctg caactggccc gtaatgaaaa ccatgcagca cgtcacggcg
    35281 ttgataagct gctggaagtt acgccacgcc atccggaagt attacgtctg gcggaacagg
    35341 cgtatatccg cactggtgca tggagttcgc tgctggatat catcccatca atggcgaaag
    35401 cccatgttgg tgatgaagaa catcgtgcaa tgctggaaca acaggcatgg attggcctga
    35461 tggatcaggc gcgtgccgat aacggtagtg aaggtttgcg taactggtgg aaaaaccaaa
    35521 gccggaaaac gcgtcatcag gtggccttgc aggtggcaat ggcggaacat cttattgagt
    35581 gtgacgatca tgatactgcc cagcaaatta tcatcgacgg cctgaaacgc cagtatgacg
    35641 atcgcctgct gctgccgatc ccgcgtctga aaaccaataa cccggaacag ctggaaaaag
    35701 tgctgcgcca acagatcaaa aacgtgggcg atcgcccgct gttgtggagc acactgggtc
    35761 agtcgctgat gaaacacgga gaatggcagg aagcatcgct cgccttccgc gcagcgctga
    35821 aacaacgtcc ggacgcctac gattacgcat ggcttgccga cgcgctggac agactgcaca
    35881 agccggaaga agccgcagcg atgcgtcgcg acggtttgat gttaacgttg cagaacaccc
    35941 cgccacagta gctctttctc acccggaggc aagcacctcc ggggcctttc tgatatataa
    36001 aaaaacgcct gctcttatta cggagcaggc gttaaaacag gtctgtatga caacaagtgg
    36061 gtgcttcact caacgttgtg tccatggtgt ctgatgaggc ataagcgaca tctgtcagtg
    36121 gacgataagc accgtaaacg gctctgcgtc attccggagt ttatgaggca ctaaggcgaa
    36181 cataagagat ggaatgagca tctactcgtt tattatgcca cagagaaccg ggaaataaca
    36241 tcccttaaca cttgttatga gataattctg taatcctctt tacttcctga gtaataactt
    36301 cctgagtgaa tatttctcct gcgcacaatt ctacacatat atataatgaa taataaaagt
    36361 cattcaatta ataacatata tattaattgc cgttaaaact aaaaacagca tcaataatca
    36421 acgcgatata acatcaccgc cttacatatc gcctgcgcca gaggtaggat tgcaaacgct
    36481 ctcctgattt tccaattcat tttctggata aataaataat ttatttttac cactattatt
    36541 tatgcaatca tcctgtcagg gagagggatc tcaattatca atgcttaatt acgtcatcat
    36601 tttgacgaca tgaaaccgga gcattttgca tggaattttc gttttcaccc aaacttcttg
    36661 ttgttgctgt cgccgcagct cttcctctca tagccagcgc agcagatacc ccgtcaaccg
    36721 ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatctg gttaaaccgg
    36781 cgaccactat tgccgacaac atgatgccgg tgatgcagca tcctgcacaa gacaaagaaa
    36841 cccagcagaa actggcggaa cttgagaaaa aaaccggtaa gaaaccgaac gtggttgttt
    36901 tcttgctgga tgatgtgggc tggatggacg ttggctttaa cggtggcggc gtggcggtgg
    36961 gtaaccctac accagatatc gacgccgttg ccagccaggg gctgatttta acttcggcgt
    37021 attctcaacc gagttcttcc ccgacccgcg ccaccattct caccggacaa tactccatcc
    37081 accacggcat tctgatgccg ccaatgtacg ggcaaccggg cggtctgcaa gggttaacca
    37141 cgctgccgca gttgctgcac gatcagggct acgtcaccca ggctatcggg aaatggcata
    37201 tgggggaaaa caaagagtcg cagccgcaga acgttggctt tgatgatttc cgcggcttta
    37261 actcggtgtc cgatatgtat accgaatggc gtgatgttca tgtcaacccg gaagtcgccc
    37321 tgagcccggc tcgttctgaa tacatccaaa aactgccgtt cagcaaagat gacgttcatg
    37381 ccgtgcgcgg cggtgaacag gaagccattg ccgacattac gccgaaatat atggaagacc
    37441 tggatcaacg ctggatggaa tatggcgtta agttcctcga caagatggcg aagagtgata
    37501 agcctttctt cctctattac ggcactcgcg gctgtcactt cgataactac ccgaacgcca
    37561 aatatgcggg tagctctccg gcgcgcacct cttacggcga ctgcatggtg gagatgaacg
    37621 atatcttcgc caatctgtat aaagcactgg agaaaaacgg tcagcttgat aacacgctga
    37681 ttgtgttcac ctctgataac ggtccggaag ccgaagttcc gccgcacggt cgcacgccgt
    37741 tccgcggtgc gaaaggttct acatgggaag gcggcgttcg tgtgccaacc ttcgtttact
    37801 ggaaaggcat gatccaaccg cgtaaatctg acggcattgt cgatctggcg gatctcttcc
    37861 ctacggcgct ggatctggcg gggcatcctg gggcgaaagt agcgaattta gtaccgaaaa
    37921 ccacctttat cgatggtgtg gaccagacat ctttcttcct gggaacaaat ggtcagtcta
    37981 accgtaaggc cgagcactac ttcctcaacg gtaaactttc tgctgtgcgt atggatgagt
    38041 tcaagtatca cgttctgatc cagcaaccgt acgcgtatac ccagagcggg tatcagggtg
    38101 gattcaccgg cacagtaatg caaacggcgg gatcgtcggt gtttaacctg tacaccgatc
    38161 cgcaggaaag cgactccatc ggcgtgcgcc atatcccaat gggtgtaccg ctacagaccg
    38221 aaatgcacgc gtatatggag atcctgaaaa aatatccacc acgcgcgcag attaaatctg
    38281 attaagccgg cgctatcgct acatcgggcc acactgacct tgccggatgc ggcgtgaacg
    38341 ccttatccgg caatcaaccg caggcggcca caaaatggcc gcttttttac ttattcacca
    38401 ccatcaaatg cgcctgcata atgtcgctgg ccggacgacc gtgcgccagc aaatcagcca
    38461 ttgctttaag atatggcggt agatggcgga aataacgctg atacccggca cacaaataat
    38521 tcagcccggg tttgccgctg gcatcgagca taaagcggtg tttcggacag cctccccagc
    38581 acgcttttaa cacgttgcaa ctgcgacact gtgccggtaa ctgcttgaat ttatcttcac
    38641 caaatgcctg ctgttgcggg gaatcaatca tttctgcaat cgtctgctga agcatattcc
    38701 ctagtcgata ctgcggataa acatagtgat cgcaggcgta aacatcgccg ttgtgttcaa
    38761 caatcaccga gcgcccgcag gttggctgat gatggcaaac cgcgcccggt gcgccgacaa
    38821 aattggcgaa cgcccattcg atattcatta cgaaaatctt accgacgtcg cgcttaatcc
    38881 agtggtcgaa aatcgccacc agaaactcac cgaactcttg cgggcacacc gaccattccg
    38941 tcagttcgcc ctgaatatcg ccgggagcat gtagcttaag tccagcatga gcagccgttt
    39001 catcagccag gcgctcgacc accggaataa actggatgaa ctcaacacct gcatcacaca
    39061 gaaaatcata gacttgcaaa ggttgtaacg cactggtgcg attgacgcag accagcacgt
    39121 tatagtcgac atgatgtttt tgcaggagcg tcagagcacg catcaccagc ttatgcgttg
    39181 gtctgccacc tttggtcacg cgatattgat tgtggatctc agccggacca tccagcgata
    39241 accctacaag aaaatgattt tctgccagaa atgcacacca ttcgtcatcg agcagcacgc
    39301 cgttagtctg gaagctatta cttatcttcc tgccagcacc atatttcgcc tgtagcgcca
    39361 cggcacggcg gtaaaaatcc agcccgagta acgttggttc gccgccctgc caggtaaaag
    39421 ccacttcgtt ttgggtttcg ctggcagcga tatagtgacg gacatacgct tccagcgtgt
    39481 catcgtccat atgcgtgact ggcttttcgt ggtaaaggga ttgtttttcg agataaaaac
    39541 agtagtcaca gttcagatta caatcagaac cgctcggttt cgccatcaca tgaaaagcac
    39601 gcgttggaac ctgttgctgc atggtcgctc cttgcctgaa gtcatccttc aataagttaa
    39661 gggcgaaatc ataccgtggc gcaatgcgtt attacgggcg tttattgcga agggaaagtg
    39721 acgaaatgca cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg gtcggcgaga
    39781 gaggattcga acctccgacc cactggtccc aaaccagttg cgctaccaag ctgcgctact
    39841 cgccgaaata ctgctttttg aatttttagt tcaattcttt aaagtcgtgg tgcgaggggg
    39901 gggacttgaa cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat
    39961 tccgccacct tcgcattgtc acaacttcta ataatggggt ggctaatggg attcgaaccc
    40021 acgacaactg gaatcacaat ccagggctct accaactgag ctatagccac cactacaaat
    40081 cttgttacgc ggtattacta ccaccgcagc tcaagcgccg ggactaaatg gcgcgcccga
    40141 caggattcga acctgagacc tctgcctccg gagggcagcg ctctatccag ctgagctacg
    40201 ggcgcttagc gccgttgcgg gggtggataa tacggacttc ccacccctct gtctagtccc
    40261 tttttaaata aattgcgcgt ttggttacgg tttgtgcgtt ttgctgctta ttcctccagt
    40321 ttatgcgctt taccgtggcg attaagccca aaaactttat aaatcgccgt caccaccagc
    40381 ataaagatga tgccgacaaa cagcgacata cgcgtatctt cattaaaata catgccgatc
    40441 aaaacgcaaa tcaggaatgc cattgttacg tagttggccc aagggaacag gattgagcgg
    40501 aacggatggc tggcaattgc tgctttatgc gcacgacgaa aacgcagctg gcttatcaat
    40561 atcacaaacc atggcaccat ccccggaagc acgctggcac tgtagacgta aacaaacaca
    40621 cgctgcggat tgggaatgat gtagttcagg catgagccaa ttagcagaat agcaatagat
    40681 actgccacac ccgcaaccgg tacgccgtga cgggaaactt tcgccattgc cgccggtaac
    40741 tgacggtttt tcgccagtgc atagagcata cgtccgcaac tgtacatgcc gctgttacag
    40801 ccagagagcg cagccgtcag caccacaaag ttgataatgc ccgccgctgc ggtaatgccg
    40861 attttggcga aagtcagtac gaacgggcta ccgttgctgc ctatttcatt ccacgggaag
    40921 atagtaacga taacgaaaat cgcgccaacg tagaaaatca ggattcgcca cagcaccttg
    40981 cctacggcac tgcgcagcgt cacctgcgga ttcttcgctt caccggcagt aatgccaatc
    41041 agctccacgc cctggtagga cgccaccaca atacacaatg cggtcaggaa ccctttccag
    41101 ccaccagcaa agaaaccgcc atgctctgtg agattgctaa aaccaatcga ctgcccgcca
    41161 ttgccaaagc caaagaaaat cacgcccagg ccaatgacaa tcatcacgat aatcgtggtg
    41221 actttgatca tcgcgaacca gaactcgatt tcgccgtaca acctcaccgc agccagattc
    41281 gccaacgcca ccagcgccac cgcgatcaat gcgggtatcc actgcgccat ctccgggaac
    41341 cagaactgga cataaacgcc aatggcggtg atttctgaga tccccaccgc catccacata
    41401 aaccagtaag accaggcggt gagataacca aagaacgggc tcatataacg atgcgcgtaa
    41461 acggcgaacg aaccggtaac cggttcgagg aacaacattt cgcccattga acgcatgatg
    41521 aaaaagacga acagcccggc gatgatatag gccaacaata cggatggccc ggcccatttc
    41581 agggtactgg cggcccccat aaacaggccg acgccaatgg tgccccccag ggcgatgagt
    41641 tcgatatgtc gagcttccag cccacgctgt agctctggtt tgttatctgc cataaatcct
    41701 cgtgttgtgt ttgcatgctt tccggtgtta ccggttatcg ttatgggtac atcgagtgtt
    41761 gcgaatgttt tcgtaattca ggagaaatgg caaataaagc attaaaaatt tgaatgcttt
    41821 gtgtaataaa aaagcagaca ggcgacgaag tgaccacttc gtcgctttac aaagagagga
    41881 aaatcagagg ttgccggtgt aatgccagcg taaataacgc agcaaacgaa gctgacgctt
    41941 aatgcggctc ggctgcgaaa gcaggcggta gagccactcc agccccagcg tttgccagat
    42001 tttcggtgcg cgttttacgt gaccggtgaa aacatcgtag gtcccgccaa cgcccatata
    42061 cagcgcatct ggatgcacca gacggcagtc gcgcatgaag atctcctgct ttggcgatcc
    42121 catcgcaacg gtgacgattt gcgctccgct ggcatgaatg cgttcaaaca gcgcctgacg
    42181 ctgctcgggt ttaaaataac catcctgact gccaacgata ttcacattcc actggttgcg
    42241 cagtttagct tcagtttgcg ccagcacttc aggtttaccg cccacaagaa ataccggtgt
    42301 cccttctttg cctgcgcgcg ccatcagctc ttcccagaga tcggccccag caacgcggga
    42361 aacctgcgcc tgcgggtact ttttacgtac tgaacgtaca acgcttatgc catccgcata
    42421 tttaaattcg gcagcgttaa ttagctcccg gacctcggcg ttatcttcaa tggtcagcat
    42481 tttttcggca ttaatggcaa ccagcgttcc ttgcttaagc tgcccgtcag cgaacagata
    42541 atcgagggcg tgctgcatat cacgccaacc aatcaactgt aagccacgca gcgtataggt
    42601 cggtgccgtg gtgttgttat tcattattat ccttcaacct gcgtccggag cgatgatttt
    42661 gttcgtttat gaatgagtcc ggcgctttca aaaagccagt acaacagttt tgcgatcatc
    42721 agacatgcgc cgaagaccac gataaaaaag accacgcgtg agacaaacga atccagccct
    42781 tcacgcgcca gcacgatcat attgaagatc gccccgaagc agaaactgtg caatatcgca
    42841 gccttatagc gattagtctc gcggttgccc agctcataca gccagtcgaa ccatttgata
    42901 atcagtccaa ccacaatcgc cccgagcggg atgaacaacg cgccgcccat caccaccagt
    42961 gagcctataa gcgtaggcga gatcgccagt ccggagtggt tattcagcac ttcccaggta
    43021 aagtagttgg cggagttcag caccatactc gggcgacccg gccacagcca ggaagggata
    43081 aagacataga aatcgcggac aatgggagcc aggccctgga agtcgatgtt gtcgtagttc
    43141 tgcaacagca acgccagatt ctcccacggc gagaaggtgt cgcgagtgag atagagaaac
    43201 gtatagaacg cttcatcgcc gctcacattc attccatagc gttttagcgc cagccagaac
    43261 atgcccacaa tccccagcac gcccgccgcc gccagcatcc acaacgaaat ccagccgcga
    43321 ataatgccaa taaacaggaa gatagcgaat gcgatgatga tattggcgcg agtgccgccg
    43381 acaatcatgt aagtcaacaa gccaaaggca accgtgctga cgaggaaaaa cagccacgct
    43441 ttgctatcct ggcgcagaaa ataaaccacc agcatcgccg ggatgaaaaa gtaaaagaag
    43501 cgttttaatg ccacgccgga gacttcgctg gaaaagatct gactgtaaga attaagccgg
    43561 aacagcaaaa agccgttgtg catgaagaag atgccgacgc tcaccagagc gatgaccatc
    43621 aggatcaccc acgtgagatt ggtctccacg cggttcatgg taaacagcgg acggcgcggt
    43681 gcatcagcaa cgcgtttgcg tagacgggtt ttgtaggtga catagtaaac cgcgtagaag
    43741 cagcctgcag aaagcaacgc ctgcaacaag atttctggcg gcgcgacacc aacatcaaag
    43801 cgaaatacca gcacgctggt cagcggaaag ccgaagaaaa aggtgagcaa aaacagcaat
    43861 gaaaagaaga cattgaagtt aaagcgcaca cggcgaaact caaaccaggt cagcgtggca
    43921 ataaacagcg tgcagagcag ccagacaaca aacaggccac tgaattgcag cagactcatg
    43981 cgacctcccc ggcggcaatc gccaacgccc gctgccagcc ttgtagatag ttagggctaa
    44041 agaaggcaat ggtgttttta tccaccgacg ccaactggcg ctgcgcttca cgcacaatat
    44101 cctcgttgag atcatcggta gtaaacagta ctggcaaatg ctgttccgtc atatcctgcc
    44161 agaacggatt ttcccggtta agcacacaag gaatgcccgc ctgaatcagt aagcaaagcg
    44221 taccaatgcc ctgctggcgg gcaaaaataa agtaaccgag atcgcactgg cgaagtagcg
    44281 tcagataggc gtcaaattcc agtttttcgc tcagaacttg taggttttct tcgctgaata
    44341 actccagtcc cgcctgacga acttcctcga tgtacgcttc gttattaggc ggatatccca
    44401 tcggcaccac cacttttacc gtatcgccaa attgctgatg aacggcgcgc aaggcagcaa
    44461 tatgctcatt gctgcggtca ccggagttcc ccaccagaat ggtcattttt ccttcacgtt
    44521 gccgatcgtt cgccatcgta ttaagagaag ggtccatccg cgtcgggaag tacagcagtt
    44581 ctccccgcac ctttggatgc gttttggcaa aaaagctcaa atcaccgcgg gtggcaaata
    44641 cacagccgac tctcttttgc gccaggcgac gtagcgggta aaaaagctta tatctcaagc
    44701 cactggaaag ctcatacagg tctgctcccc agatatgcca gaaaaactgg ctgggcttaa
    44761 tgccaccact cagcagagcc agccacagtt tgggattgaa ctgaccgtgg aaaaaaaatc
    44821 gctgctgacg gttagctttt gctttcgcga tgaccgcttc cgccagcgat tttttcccag
    44881 ggaaaaattg cacagaaagc gccggacagc tatcacttaa gccgtcgtcc ttgccagcaa
    44941 ccataaactc gtgcgcgtgc tcgctcgtcg cggccagcgc gtcgttgaaa aaccgcagaa
    45001 cggttcggtt atggtgaggg atatccgatc ccagtacgtg aatcagtaca gtcatgcccg
    45061 cctacgccag agtaaaaaca cgccacaaca aagagaaaaa tagacgatat aggttgccat
    45121 ataggcctgt gctgcgccca gtgcgccatg cgcagggatt agccagtgag caaataccat
    45181 caataaagtg aactggctga tttccgccag aatataaaac cgcagtgacg ctttggcgat
    45241 caccagataa ccaaagacat aagcgcccac ttttaacaca tcacccacca actgccaggc
    45301 aaagagatcg cgcatagcgg taaatttatt cgacaacagc agccagatag caaaatcacg
    45361 cagcaaccag acggtaaaac tcgccgccgc cactgccggt aagacgaatt tcagcgattt
    45421 aaccacttcc cgggtgatat cgcgcttttc cgttaaccgc gacaacgtgg gcaacaaata
    45481 tacgctgaac gatgccgtaa taaattgcag gtaggcatcg gaaatactgc tcaccccttg
    45541 ccagatcccc acttcatccc agctatactg cgccgccagc agtttacgca tcatgatgta
    45601 agcaacaggc aaggtcaccg acgtaatcaa cgccatgagc gtaaatttgc tcaactgccc
    45661 tgccagaccg ttatcccagc tgggtttcag atagcttaac gggatgacac cacgtttgat
    45721 caacatgata acagcaggaa ttaccaccag cgcgggaatc agcgccagac ccagcaacgc
    45781 cccttcataa ccgcccaaac gataactgac gtagtacgcg agaacgccaa tcaagctacc
    45841 gacaatcaga gataacgcat tacctgcggc atcgcgaaag cctttcatca gcgccagtaa
    45901 caggttgccc caggcgatcc ccatttgcac cagcgccact aaacgcacca gcccctgata
    45961 gtcggtatta ccaaacaatc cctggctgat tggcgcagct gccagcacaa aaaccagcgc
    46021 catcagcgta gagaagccaa gtaccatcgc tgatgaagtg ccaaccacgc ggcgcagttg
    46081 ttggggatta tcatggtact gggcaacgta tttggttacg ccgttaaaga tgcctgcccc
    46141 ggcaagcacg ccgagcacgg taatcaactg gcggaaattt gccgccagcc caagccccgc
    46201 cggaccaaat gacactgcca gcaacttacc gaccagtaac ccggcaccaa tcttgaccag
    46261 tgtactggcc gccgtccaca aggacgcttt tgccaacgac atatcaggag aagtagttca
    46321 acaaggtcgc aattaccgta cgctgattga cgggcgacag gttgtagaac aacggcaggc
    46381 gcagcaggcg ctcgctctct ttggtggtgt agcgatcttc accgtggaac tcaccaaagc
    46441 gttcccccgc agggcaaccg tgcagcggaa tgtaatggaa caccgccatg atttccgctt
    46501 ctttcagaaa gttaatcaat gcgctccggt catcaatatc ccgcagttta atgtagaaca
    46561 tatgcgcgtt ctgcacgcag ccatcgggaa tcgacggcag ctcgatacgc ccggctttcg
    46621 ccagaggtgc taacgcatcg tagtagtttt gccacagcgc cagacgctgc tgattgatac
    46681 gttcagcggc ttccagttgc gcccacaggt acgcagcttg cagatcggac atcaaatagc
    46741 tggagccaat atcgcgccag gtatatttat cgacctgacc acggaagaac tggctgcggt
    46801 ttgtgccttt ttcacggatg atctctgctc gttcgatcaa cgctttatcg ttaatcagtg
    46861 tcgcgccgcc ttcaccaccc gccgtgtagt ttttggtttc atggaagcta aagcagccaa
    46921 tatgaccaat ggttcccagc gcacgccctt tgtaagtgga catcacgccc tgagcggcat
    46981 cttccaccac aaacagatta tgcttcttcg ccaacgccat aatggtgtcc atttcgcagg
    47041 ccacacccgc gtaatggacc ggcacgataa cgcgcgtttt gtcggtgatc gccgcttcaa
    47101 tcagcgtttc gtcgatgttc atggtgtccg ggcgaacatc cacaaagacg attttcgcgc
    47161 cacgcagcac aaaggcattg gcggtggaga caaaggtgta gctcggcatg atcacttcat
    47221 cgccaggctg gatatcgagc agcagcgccg ccatctccag cgaagcggtg caggatggcg
    47281 tcagtaacac tttggcgctg ccaaaacgtt gctccagcca ctgctggcag cgacgggtaa
    47341 aaccaccatc gccgcacagt ttgccgctac ccattgccga ctgcatatag tcgagttcgg
    47401 ttcccaccac cggcggtgcg ttaaatggaa tcatgtgatc acctgtataa ccagtacgcg
    47461 gtgctttcta cattcgcacc actttgtatg tatcgtttaa gcgcggcggt gttgcccatc
    47521 tgggtcgcca cccgcaaagt tgttttaccg cgagcatacg cccagtttag cgccgtttgc
    47581 attagctcag cacctgcacc gcgtccagcc agcaggccaa ttcgcgcatc tgtcgcattg
    47641 agttcccgta aagagacata accgcgaata tcgccggacg ccgcacgtaa aatcagacat
    47701 tgatgatcaa aggtgccgcg cacggcattt tcaatccact gtgcataaaa gcgaccgctg
    47761 gcgtcaggcg cataccacgg cgcacgaaaa cggctttgcg caaatgcggc gctggctaac
    47821 tgacgtaatg cgggaatatc ggtctcttgt gccactactg caccgctatc actgacattg
    47881 ttcacgggta gtgccaaatc aacttcacct tctaccaggg agaatcccag ctgttgcagg
    47941 gcatccagtt cacccgtatt tgatgccgca attttggcct gcacccgtga ccacggcgct
    48001 aacgcgtctg gcgtcaggag tgccgcgtca ggactaatac acacaatggc gctgttaaca
    48061 ccaaagaagg cgttttccca ggttagtggc tcaatactgg cgcggacggg cacgaagtaa
    48121 ctccagcaga tattggccgt agccagtttt cgctaatgaa ctggcagcac gcttcacacc
    48181 ctcgtcatcg agccagccgt tacgccaggc aatctcttcc aggcaggcaa tcttaaagcc
    48241 ctggcgtttt tctactgtct gcacaaaagt gctggcctca atcaggctgt cgtgagtgcc
    48301 ggtatccagc caggcaaatc cgcgcccgag cagttcaacg gtcaggttgc ccgcctcgag
    48361 gtacatctgg ttgatggagg taatctcaag ttcaccacgc tccgacggct tcacctgctt
    48421 tgcgtactcc acgactttac tgtcgtagaa ataaagcccg gtcaccgccc agtttgattt
    48481 cggctgtttt ggtttttctt ccagcgagat agcgcggaaa ttgtcgtcaa actccaccac
    48541 gccaaagcgt tccgggtcca tcacctgata gccaaatacc gtcgcccctt ccgtgcgcgc
    48601 cgcaacatga cgcagcttcg gactgaagcc ctgtccgaag aagatgttat cgcccagcac
    48661 cagacaagaa ggttcaccgt tgaggaaggt ttcaccgatg ataaaggcct gcgccagacc
    48721 gtccggactg ggctgttcgg cgtattccag ctggataccg aactcactac catcgcccag
    48781 caggcgctgg aaataacctt tatcttccgg cgtagtgatg atgagaattt cgcggatacc
    48841 ggccaacatc agcaccgaca gcggatagta aatcattggc ttatcgtaaa tcggcaacag
    48901 ttgcttcgat acgccgcgcg taatcggatg caatcgggtg ccggaaccgc ccgccaggat
    48961 aatacctttc atgtttgcct ccgctgaaaa ttagcctttc agacctaaac gctcgccctg
    49021 atagctgccg tcctgcacct gcttacacca gctttcatta gccagatacc actgcaccgt
    49081 tttacgcatt ccactttcaa aggtttcctg cggcagccag ccaagttcac gggcaatttt
    49141 cgaagcatca atggcataac gcagatcatg ccccggacgg tcagcgacaa aggtgatcag
    49201 gtcacgatag tgcgccacgc cgtgcggctt attcggagcc agttcttcca gcagctcgca
    49261 aatggtttcc acaacatcga gattcttacg ctcgttgtga ccaccaatat tataggtttc
    49321 accgactttc cctgtggtcg ccacgcaata cagcgcgcgg gcgtgatctt caacatacag
    49381 ccagtcacgg atttgctgcc cgttgccata taccggcagc ggtttacccg ccagcgcgtt
    49441 gaggatcatc agcgggatca gtttttccgg aaagtggtaa ggaccgtagt tattcgagca
    49501 gttggtgata agcgtcggca aaccataggt ccgcagccag gcgcgcacca gatggtcgct
    49561 gctggctttt gacgcggaat aagggctgct cggcgcatac ggcgtggttt cggtgaagaa
    49621 atcatccgtc gagtgcaggt caccatatac ttcgtcggtg gagatatgat gaaaacggaa
    49681 cgctgatttt ttatcttccg tcagcgcagt ccagtaagcc cgcgccgctt caagcagtgt
    49741 ataagtcccg acaatgttgg tttcaataaa cgctgccggg ccgtcaatag aacggtcaac
    49801 atggctttcg gctgccagat gcatgacaca gtctggctga tactcagtga atacgcgcgc
    49861 cagttctgcc cgatcgcaga tatcaacttt ctcaaaggca aagcgctcgc tttgcgcgac
    49921 cggtgccaga gacatcaggt ttccggcgta ggtcagctta tcgaccacca ccaccgcgtc
    49981 gctcgtttcg ttgatgatat aacgcaccag cgccgagcca ataaacccgg caccacctgt
    50041 tatcagaatc tttctcatca gcgccagact cctttggcat cgacgacata ctgctgatgg
    50101 acgttgtcgc cattgataac tttgaactga ctatgatcga ccagcatcac cagcacatct
    50161 gccgttgcca gcgcctcgtc aagctgcgcc agagtacaaa gcccggtcag tttcttcggc
    50221 aactcgtgga tgttaggctc aacaaccagc gtttcgccgc tgtgccactg ggcgatcagt
    50281 tcagcgattt ccattgccgg actttcgcgc aggtcatcaa tattcggttt aaacgccaga
    50341 ccaaagcagg cgattttcag ttcactggcg cgtttatcgg tagcagccag acaatcagcc
    50401 accgccgctt tcacctgatc gataacccag aacggtttgt gatcgttcac ttcgcgcgcg
    50461 gtacggataa gccgcgcctg ctgggggttc tgtgccacga taaaccacgg atcaacagcg
    50521 atgcagtgac cgcccacgcc agggcctggc tgaagaatat tgacgcgagg gtgacgattc
    50581 gccaggcgaa tcagttccca gacgttaatc ccctgatcgg cacaaatcag cgacaattca
    50641 ttagcaaaag cgatattcac atcgcggaag ctgttttcgg tgagcttaca catttccgcc
    50701 gtccgcgagt tagtgacgac acactcacct tcgaggaaaa ttttgtacag ttcgctggcg
    50761 cgggccgaac aaaccggcgt cataccacca atcacgcgat cgtttttaat cagctcgacc
    50821 ataacttggc ccggtaatac gcgttccggg cagtaagcaa tgttgacgtc cgccaactcg
    50881 cccacctgct gtgggaaagt gagatctgga cgcatctctg ctaaccattc tgccatcttc
    50941 tcggttgacc ccaccggcga ggtggattca aggatcacca acgcgccttt tttcagcact
    51001 ggcgcaatgg agcgagcagc cgattcaacg taggtcatat ctggctcatg atcgccttta
    51061 aacggcgtgg gtacagcaat caaccaggca tccgcttcaa ctggcgtcgt gctcgctcgt
    51121 aaaaaaccgc cttctacggc agtttttact acgctcgcca aatcaggctc gacgatatgg
    51181 atttcgccac gattgatggt atcaaccgcg tgttggttga tatcgacacc aatcacctgt
    51241 ttttgccttg aggcaaacgc agctgctgta ggcagcccga tgtaacccag tccgataaca
    51301 gaaatggtcg caaaactcat agtgatatcc gattattttt taacgcttcc agaatgcgag
    51361 agcatgcctg accatcacca tacgggttat gggcgcggct catagcttga tattcgtttt
    51421 cgtcttttaa aagacgcgtc acttcctcga caattcgctg cttatccgtg ccgaccagac
    51481 gcaccgtacc cgccgtcacc gcttccggac gctcagtggt atcgcgcatc accagcaccg
    51541 gtttccccag cgaaggcgct tcttcctgaa tgccgcctga gtcggtcaaa atcagccagg
    51601 cgtggttcat cagccagaca aacggtaaat actcctgggg atcgatcaga atgacatttt
    51661 tcacatgccc cagaatgcga ttgaccggct ctctgacgtt cgggttgaga tgcaccggat
    51721 agacaatctg gatgtcctgg tgcgtggtgg cgatgtctgc cagcgcatgg cagatttctt
    51781 caaaaccacg accgaagctc tcacgcctgt gaccggtcac cagaatcatc tttttatcgg
    51841 gatcgataaa cgggtaattt gccgccagtt ctgaacgcag cgtatcgctg ctcatcacct
    51901 ggtcacgcac ccataacagt gcatcaataa ctgtattacc ggtaatgaag attcggctat
    51961 ccgcaacgtt ttcacgcagc aagttttgcc gggaagtttc ggttggagag aagtgataca
    52021 tcgccagatg cccggtcaat gtacggttag cctcttccgg ccacggcgaa tagagatcgc
    52081 ccgtgcgcag accagcctca acgtgaccaa caggaatacg ctgataaaac gccgccaggc
    52141 tggttgccag cgtcgtcgta gtatcgccgt gaaccagcac gacatctggt ttgaactcgg
    52201 caagaatagg ttttagccct tccagaatcc gacaggttat ctctgtaagg ccctgtcctg
    52261 gctgcattat gttgagatcg tagtcaggta caatggaaaa gagtttcagc acctgatcga
    52321 gcatctcccg atgctgcgca gtgacgcaaa ctttagcctc aaaaaaagga tcttttgcca
    52381 acgcatgcac caacggcgcc atcttgatgg cttccgggcg cgtaccaaat acagtcagta
    52441 ctttcacatc gattctcttc gaataagcgg cgagcgcctt tgcgctcacc gcagcagtgt
    52501 tgctatttcg agcaacggcg ggttaatgcg acaccagccc cgatcagtcc cccgacaatg
    52561 ccccacataa tcatcatgaa ggcacgacgt gggctatcgc gttttaccgg ttcttccggc
    52621 gtacgcaaat agcgataggt ctgaaaacgc ggatccaggg ttggaccaac attcagggtg
    52681 tttaacatgg cccgattctg atcatagtcg agatcaaagg ccggaccgac ggcctgtaaa
    52741 ttttccagtc gagcctggag cattggacgc ccaagcagga acatttctga atcaggtaat
    52801 tcctcggcag gcacatctgt cgcactgcgc gaaatattat gctgctcagc aattttcagc
    52861 gcctgttcaa tactgttcat ccggcgatcg tagatggctt tcgccacctc ttcctgacgc
    52921 ttcacctgag ctttcatctg gatggtgcgt gccgcccatg cgcctttcag ctcatcattc
    52981 agatggctgg ctgcacgctg gctggcaaaa gcaacatact gacgtaacag gttattagcg
    53041 tcaggcgcgg tttcggcaat aagcttcacg ctgtcattga ccgcgcgggt aaagtcaccg
    53101 ggaataaaca cgatgttgtt aatcatttca tccagcaacg ccgcatcggc tttgctgttg
    53161 cccaccatcc gctgtttgta atagtcggtt tgcagccaga actctctgcg ggtatcccac
    53221 gaggccagtt gcataacaaa ctctttataa gcttcgtcca tgaccgatgg ttggtcggca
    53281 gaagccatgt ttgaacggac atccaggtta cgcaaaaatt gctgctgcga gtaatatccc
    53341 cccagcatat tcaccgttgg acgatcggta atcgctgtcg agctccactc ctggcgagca
    53401 aaaaaagtat acgccagcgc aattaacgca aacgccagcc ccatgccaat aatccacagc
    53461 ttcccagccc acaaggtacg aaacaaccca cgaatatcca gttcattttc agcgtcttcg
    53521 gccggtttcc caggcattgg ttgtgtcatc acatcctcat ttatttggtt aaattggggc
    53581 tgccaccacg atttctacgc agtctgcgtt ttacgcgctt aataaagcga gcaactttcc
    53641 aggcacgctt aatgcaatat ccatagagga agaatgctag caaaaagagc accagcatga
    53701 cccactccgg gacaaaatga gaatattctg ccagcacgcc aatggaagcg agcagtgctg
    53761 cggcaagggt aatcagcaca aacgcctggc gggaagtaaa cccggcacgc atgatcaaat
    53821 ggtgaatatg ctgacggtct ggagagaatg ggctcatgcc tttacgcagg cgacggtaca
    53881 taatcgccac catatccatt agcggaatgg cgattatcca caaagcggta accgggctga
    53941 tgggatgagt tttgccctgg gtcgtttcga gcaggatcca gataacggta aaaccaatca
    54001 gcgtactgcc cgcatccccc ataaagactt tgtagcggcg acccaggata ccaaggttaa
    54061 gcatgatgta tggcaggatg gcggcgatca tcgcaaagca ccagattgcg agactggttt
    54121 gcccgtcgaa ccacaaaatc ataccgattg ctgcaaacga gacgcaggac aacccgccca
    54181 gcaagccatc aatgccatca accatgttga acgcattaat ggccgcccag acggcaaata
    54241 gcgtcaggaa gtaaccaaac ggtccgagca ccatctccca ggagccaaag atataaccca
    54301 ggctactgag ataaagtttg ccgaacacca tcataacaat gccaacagcg gcctgtatgg
    54361 tggcacggat ttttacgctg atatcaaaac ggtcatccag cgcgccaatg aaaacaagca
    54421 caccagcaca agcgagatag agagatgcat gcggaatata gtaatcaaca attccgaacg
    54481 tgaagcaaat ccctgcgtaa accgaaatcc ccccaacgag aggtatcaat ccctggtgac
    54541 gtttgcggaa gtttggttta tccactaaac cgactttttt tgccacctta cgggcaaaaa
    54601 acagaaacag tgtcgtgaat aaaaaaatac tgatgagatc agtactcact gtcagtaaat
    54661 tcacaatgca tgctctcaga gaaaattatt agcagaagta taaccacgaa gacctttatt
    54721 cagaagggaa agtccgttcc gaacccacca gtcctgtgca aaaattaacc atttgatatg
    54781 caaattgcat aacacaccag caaattccag gaattttcct agcatcgcca gcgcggcatt
    54841 aagattacag atataaaagc aaaacgccac gtaaacacgt ggcgtttttg gcataagaca
    54901 aatttatgag cgtttcatca tttcgaagaa atcgtcattg gtcttggtca ttgccagttt
    54961 attaatgagg aattccattg catcgatttc gcccatcggg tgaatgattt tgcgcaggat
    55021 ccacattttc tgcagttctt cctgagtcgt gagcagctct tctttacggg taccggaacg
    55081 gttgtagtcg atagccggga agacgcgttt ttcagcgatc ttacgagaga ggtgcagttc
    55141 catgttgcct gtacctttaa actcttcgta gataacttcg tccattttag aaccggtatc
    55201 gataagcgcc gtcgcgataa tggtcaggct gccgccctct tccacgttac gcgccgcacc
    55261 gaagaagcgt ttcggacgat gcagggcgtt ggcgtccaca ccaccggtca acactttacc
    55321 tgacgccgga acaacggtgt tgtaagcgcg cgccagacga gtgatggagt cgagcagaat
    55381 gataacgtct ttcttgtgct caaccaggcg tttcgccttc tcgatcacca tttccgcaac
    55441 ctgaacgtgg cgagatgcgg gttcgtcaaa ggtagaagca acaacttcac cttttaccag
    55501 acgctgcatc tcggttactt cttccggacg ttcgtcgatc agcagaacca tcagcacaca
    55561 atccgggtgg ttgtaagcaa tgctctgagc aatgttctgc agcagcatgg ttttaccggc
    55621 tttcggcggt gccacaatca gaccacgctg accacgaccg ataggtgatg ccagatccag
    55681 tacgcgagcg gttaaatctt ccgtagaacc gttaccacgt tccatacgca gacgagagtt
    55741 tgcgtgcagc ggggttaagt tctcaaagag gattttgttg cgggcgtttt caggtttgtc
    55801 gaagttaact tcgttaactt tcagcagcgc aaaatagcgt tcaccttctt tcggcgggcg
    55861 aatcttacca gagatggtat caccagtgcg gaggttgaaa cggcggattt ggctagggga
    55921 aacgtagatg tcatcaggac cggcgaggta ggagctgtct gcggaacgga ggaaaccaaa
    55981 tccatcctgc aatatctcca gtacgccatc accaaagata tcttcgccac tctttgcgtg
    56041 ctgcttcagg atggcaaaaa taatgtcctg cttacgcata cgagccaggt tttccagccc
    56101 catattttcg ccgagagtga tcagctcaga aaccggcgta ttctttaatt cggtaagatt
    56161 catagtggtg tgagttctta aacttggggt aaatctcgaa cttaatgttg tgaatggtat
    56221 ggcaggatca tccatgcctg tttacggtca tcgactcatg tcttttcgct gcctggtcac
    56281 aggagagtac gcagaactga aacgacaaga cggattgagt gacgagccag aaatctgttc
    56341 acttcgcatt taagataaaa cgggaagcgt tggaaattac aagattcaaa cttaataagg
    56401 tatgtttaat acgaagtcaa cactaagtta gcatgactca cgccgggcgt ccagttttta
    56461 gcgacggggc acccgaacat gaaattccct tacgccaggt tagcgtcgag gaactctttc
    56521 aactgacctt tagacagtgc gcccactttg gttgccgcca cttcaccgtt tttgaacagc
    56581 agcagagtcg ggataccacg gatgccatat ttcggcgcag tgccagggtt ttgatcgatg
    56641 ttcagttttg caacggtcag tttgccctga tattcgtcag cgatttcatc cagaatcggg
    56701 gcgatcattt tgcacggacc gcaccactct gcccagaaat cgacgaggat cgccccgtcc
    56761 gctttgagta catccgtgtc aaaactgtcg tcagtcaggt gaataatttt atcgctcata
    56821 tataactcca caggaataag cctggcgtgt tggtttcgtt gttggtgtaa cattaaccaa
    56881 ctaaaggttg actttatttc accggatacg ctttcgtaaa gcaatagtaa gctgatattc
    56941 taccacacta tgagcaaaac acatttaaca gaacagaagt tttccgactt cgccctgcat
    57001 ccgaaggttg tagaagccct tgaaaaaaaa gggtttcata actgtacgcc cattcaggca
    57061 ctggcccttc cgctgacgct ggcgggtcgt gacgtagccg ggcaggcgca aaccggtacc
    57121 gggaaaacga tggcgtttct tacgtcaacg tttcattatc ttctctctca tcccgcgata
    57181 gccgatcgca aagtgaatca gccgcgtgcc ttaattatgg caccgacgcg tgaacttgcc
    57241 gtgcagatcc acgccgacgc ggaaccgctg gcggaagcta ccggcctgaa gctgggtctg
    57301 gcttacggtg gtgatggcta cgacaaacag ctgaaagtgc tggaaagcgg cgttgacatt
    57361 ctgattggca ccacgggtcg tttaattgac tacgccaagc agaaccacat taacctcggt
    57421 gccattcagg tggtggtact ggacgaagcc gatcgcatgt acgatctggg ctttattaaa
    57481 gatatccgct ggctgttccg ccgtatgccg cctgcaaacc agcgcctcaa catgctgttc
    57541 tccgccacgc tttcgtaccg ggtacgtgaa ctggcgttcg agcagatgaa caatgccgaa
    57601 tatattgaag tggaaccgga acagaaaacg ggccaccgta ttaaagaaga gcttttctac
    57661 ccttctaacg aagaaaaaat gcgtttgctg caaacgctga tcgaagaaga gtggccagac
    57721 agagcgatta ttttcgccaa caccaaacac cgttgtgaag agatctgggg ccacctggcg
    57781 gcagatggtc atcgtgtcgg tttattgaca ggcgatgtcg cgcagaaaaa acgtctgcgt
    57841 attcttgatg aatttacccg tggcgatctg gatattctgg ttgccaccga cgttgccgcg
    57901 cgtggtttgc atattcctgc tgtgacacac gtctttaact acgatttacc cgatgactgt
    57961 gaagattacg ttcaccgtat tggtcgtaca ggtcgcgcag gcgcaagcgg tcactctatt
    58021 agcctggcgt gtgaagagta tgcattgaat ttgcctgcta ttgagaccta tattggtcac
    58081 tcaattccgg taagtaaata taatccggac gcattgatga ccgatctgcc aaaaccgctg
    58141 cgcctcacgc gcccgcgcac aggcaatggt ccgcgtcgta ctggcgctcc gcgtaatcgt
    58201 cgtcgttcag gttaaagaaa aaatgtcata ctccaaatca ttcaagctgt atcaagatgg
    58261 caagagaatg aatcccgatg cgcttactgt cgtaagtgat tcaggtgaag gaatgcagcc
    58321 aacgcagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct gtatgcagcc
    58381 attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc tggaagcatc
    58441 cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa tagcgaaaat
    58501 gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt tgctgaacgt
    58561 ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt acgccttgcc
    58621 gtcaatgcgg gtgattttat tgccaaagcg caggaaatcc tcggttgtcc ggtacaggtg
    58681 atcagcggtg aagaggaagc gcgtctgatt tatcagggcg ttgctcacac cactggcggt
    58741 gccgatcagc gcctggtggt ggatatcggc ggagccagta ctgaactggt aaccggcacg
    58801 ggtgcacaaa ccacctcttt gttcagcctg tcgatgggct gcgttacctg gctggaacgc
    58861 tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa agcggcacgc
    58921 gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt gtgcgttggc
    58981 gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat ggatgaacgc
    59041 attaccctgg aaaagttgca gcaactgaaa caacgcgcca ttcattgcgg tcggctggaa
    59101 gagctagaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag tggtctggcg
    59161 atcctgatag ccatttttac cgaactgaat attcagtgta tgaccctggc gggcggtgcg
    59221 ctgcgtgaag gcctggtcta cggtatgttg catcttaccg tcgagcagga tattcgcagc
    59281 cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc acagcgcgta
    59341 gccaaagttg cggctaactt cttcgatcag gtggaaaatg aatggcatct tgaagcaata
    59401 agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag cgttgacttc
    59461 aaacaagcgc cgcaacacgc tgcttatctg gtacgtaatc tggatcttcc cggttttacc
    59521 cccgcacaga aaaaactgct ggcgacgcta ctgctcaacc agactaatcc ggtcgatctc
    59581 tcatcgctgc atcagcaaaa tgccgtacca ccgcgcgtcg cagaacaact ctgccgttta
    59641 ctccgcctgg ctatcatttt tgccagccgt cgccgtgacg atctcgtgcc agagatgaca
    59701 ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct aacccaacat
    59761 ccgctgggta aagagattat tgttcaggaa agccagtggc agagctatgt ccactggccg
    59821 ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acacattttt tccggccttg
    59881 attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg ctttgccctt
    59941 tctgcatccg ttcttcggcg ctcaccactt tgcgctcctg ttcccaaatc agatcatcct
    60001 gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagttcgccg tactgacggc
    60061 gttctttaca cagcgtaaag gtcaattcct tctgggcgcg agtaatgccg acataggcca
    60121 gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc aaaaaacctt
    60181 cttccatacc gaccatgtag acataaggaa actccagccc tttcgacgcg tgcagagtca
    60241 tcagttgtac ctgatctaac tcttcctcgc tctcgccgcg ctccatcatg tcgcgcaaag
    60301 taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg ccttccagca
    60361 tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg gcagctttcg
    60421 ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc agatcacgca
    60481 ccgcggcaat cggctctcgc tccgccaggc gctggatttc tgccaaccag tgagtgaagc
    60541 gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata tcaaagctgg
    60601 cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc agcgtagccg
    60661 ggccaatctc tcgcttcggc gtgttaacga tacgcagaaa tgcgctgtca tcgtccgggt
    60721 tagtcagcac gcgcagataa gccagcaaat ccttgatttc aggacgagag aaaaacgacg
    60781 taccaccaga aattttgtac gggatgcggt tttgcatcag gaatttttca aacacccgcg
    60841 actgatggtt gccgcgataa agaatggcgt aatctttgta ctgcgtttta ttgacgaagt
    60901 gatgggcgat cagctcgccg gtgacgcgct cggcttcatg ttcttcgtta ttcgcgctta
    60961 aaacttttag ctccgcgcca tagcccagtt cggagaacag acgcttttca aagacgtgcg
    61021 ggttattggc gatcaggatg ttcgccgctt tcagaatacg tccggaagag cggtagttct
    61081 gctcaagctt aatcaccttc agtgccggga aatcctgact caacagcacc aggttttgcg
    61141 gacgtgcacc gcgccaggag tagatcgact gatcgtcatc gccaactacg gtaaagcgcg
    61201 cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg tcctgatact
    61261 catccaccag caggtagcga attttgttct gccagcgctc gcggacttct tcattgcgtt
    61321 gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacgtta caggctttca
    61381 ggtgtgcatc atacagccca taacaatggg caaaaatacg gtcccgctcg ccctttgcct
    61441 ctgccgccgc ctgcgccggt gttttgagat cgttcttcca gttggagatg gtcgaaatta
    61501 gctgttgcag gagaactttg tcatcttcaa tcagcccctc ggtcaactct ttaagcaaag
    61561 caagctgatc ggtatcgtca aacagcgaaa agttagcctt catccccagc gccgcatact
    61621 cgcgtttgat gatatccagc cccagcgtgt ggaaggtgga aatcatcagc ccacgcgcct
    61681 ctttgcgccc cagcgtctgc ccgacacgct ctttcatctc gcgtgccgcc ttattggtaa
    61741 aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga tgggcgattt
    61801 tattggtgat aacacgagtt ttaccggaac ccgcgcccgc cagcaccagg caggggccgg
    61861 taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggaaaa tcgctcaatg
    61921 aaagtcggga ggggaaaata acagcgtggt agtatagcca gcctcatcct taccactcaa
    61981 ggcacgatca tggcaaaaac agcagcagca ctgcatatcc ttgtaaaaga agagaaactg
    62041 gctctggatc ttctcgagca gatcaagaat ggtgccgatt tcggcaagct ggcgaagaaa
    62101 cactccattt gcccgtcagg caaacgcggc ggtgatttgg gtgaattccg ccagggtcaa
    62161 atggttccgg cgttcgataa agtggttttc tcttgtccgg tactggagcc aaccggcccg
    62221 ctgcataccc agttcggtta ccacatcatt aaagtgctgt accgtaacta acagaaaggc
    62281 cttctccggg agaaggcctt gaactctgaa tcgctattaa cccgcaacag caatacgttt
    62341 catatccgtc atatagccgc gcagtttctt acctacctgc tcaatcgcat ggctgcgaat
    62401 cgcttcattt acatcacgca actgcgcgtt atctaccgca ccttccggaa tagctttacc
    62461 caggtcgccc ggttgcagct ctgccataaa cggtttcagc agtggcacac aagcgtaaga
    62521 gaacagatag ttaccgtact cggcagtatc ggagataacc acgttcattt cgtacagacg
    62581 cttacgggcg atggtgttgg caatcagcgg cagttcgtgc agtgattcat agtaagcaga
    62641 ttcttcgatg atgccggaat caaccatggt ttcaaacgcc aactcaacgc ctgcttttac
    62701 catcgcgatc atcagtacgc ctttatcgaa gtactcctgt tcaccgattt tgccttcata
    62761 ctgcggcgcg gtttcgaatg cggttttgcc agtctcttca cgccaggtca gcagtttctt
    62821 atcgtcgttg gcccagtcag ccatcatgcc ggaggagaat tcgccggaga tgatgtcgtc
    62881 catatgtttc tggaacagcg gcgccatgat ctctttcagt tgctcagaaa gcgcgtaagc
    62941 acgcagtttc gccgggttag aaagacggtc catcatcagg gtgatgccgc cctgtttcag
    63001 cgcttcggtg atggtttccc aaccgaactg aatcagtttt tctgcgtatg ccggatcggt
    63061 gccttcttcc accagcttgt cgaagcacag cagagaacca gcttgcaaca taccgcacag
    63121 gatggtttgc tcgcccatca ggtcagattt cacttccgca acgaaagagg attccagaac
    63181 gcccgcacgg tgaccgccgg ttgcagccgc ccatgcttta gcgatcgcca tgccttcgcc
    63241 tttcggatcg ttttccgggt gaacggcaat cagcgtcggt acgccgaatc cacgtttgta
    63301 ctcttcacgt acttcggtgc cagggcattt cggcgcaacc attacgacgg tgatgtcttt
    63361 acggatctgc tcacccactt ctacgatatt gaaaccatga gagtagccca gcgccgcgcc
    63421 gtctttcatc agtggctgta cggtgcgcac tacatcagag tgctgcttgt ccggcgtcag
    63481 gttaaccacc agatccgcct gcgggatcag ttcttcgtaa gtacccactt taaaaccatt
    63541 ttcggttgct ttacgccagg atgcgcgctt ctcagcaatc gcttctttac gcagagcgta
    63601 ggagatatcg agaccagaat cacgcatgtt caggccctgg ttcagaccct gtgcgccaca
    63661 gccgacgatg actacttttt taccctgaag gtagctcgcg ccatcggcga attcatcgcg
    63721 ccccataaag cgacatttgc ccagctgtgc caactgctgg cgcagattca gtgtattgaa
    63781 gtagttagcc atggtgattc ctcgtgatgt tgtgcttctt attgttcggt tcgcgtttgc
    63841 gagattgaat tcactatatg acaggaaatt tattgcggaa attgatatat tcacaacgtc
    63901 acattgcaat ttctgcaacg tcaacattaa gggctgcgcc attggattta cgcgatctga
    63961 aaaccttcct gcatctggcg gaaagccgcc attttggccg cagcgcgcgg gcaatgcacg
    64021 ttagcccgtc cacgctctct cggcagattc agcgcctgga agacgatctc ggtcagccgc
    64081 tgtttgtgcg cgataaccgc acggtaacgc taaccgaagc aggcgaagaa ctgcgcgttt
    64141 tcgcccagca aacgctgttg cagtatcaac agctgcgcca caccatcgat cagcaaggac
    64201 cgtcgctctc tggcgaatta catatcttct gctcggtgac cgctgcctac agccatctgc
    64261 cgccgattct cgatcgcttc cgcgcggaac acccgtcggt ggagattaaa ctcacgactg
    64321 gtgatgcggc agatgcgatg gaaaaggtag tcactggcga agcggatctg gcgattgccg
    64381 ggaaaccgga gactctgccc ggcgcagtgg cgttttcgat gctggagaat ctggcagtag
    64441 tgctgattgc ccccgcgcta ccctgcccgg tgcgtaacca agtttcggca gagaagccgg
    64501 actggtctac agtgccgttt atcatggccg atcagggacc ggtgcgccgg cgcattgaac
    64561 tgtggtttcg acgcaacaaa atcagcaacc cgatgattta cgccacggtc ggcgggcatg
    64621 aagcgatggt gtcgatggtg gcgcttggct gtggcgtagc cttgttgccg gaagtggtgc
    64681 tggaaaacag cccggaaccg gtgcgtaacc gcgtgatgat tttagagcgc agtgatgaga
    64741 aaacgccgtt tgaactgggc gtgtgtgtgc agaaaaagcg gctacatgag ccgctgattg
    64801 aggcattctg gaagattttg ccgaaccatt aacccgccaa aaagaacctg aacgccgggt
    64861 tattggtttc gtcgtggcaa tcgtaaccca actcattcaa ccgggtttcg aaatccggtt
    64921 catggtcgcc aagttcgaac gccgccagta cgcgcccgta gtcggtgcca tggctgcgat
    64981 agtggaacaa agaaatgttc cagtgcgtac ccagcgtgtt gaggaagcgc aacagcgcgc
    65041 ccggcgattc cgggaattcg aagctgtaga ggcgttcctg caacggatgc gacggacgcc
    65101 cgccgaccat atagcgcaca tgcagcttcg ccatttcgtc gtcggagaga tcaaccacgc
    65161 tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg ctcttcgagg ccgcggctca
    65221 ggcgcacgcc aacaaaaatg caggcgtttt tggcatcggc aaaacggtag ttgaactcgg
    65281 tgaccgagcg cccgccaagc agttggcaga atttgaggaa gctgcccttc tcttccggga
    65341 tggtcaccgc caacaacgct tcacgctgtt cgcccagttc gcaacgttcc gagacgtagc
    65401 gcaggccgtg gaagttcacg ttggcaccgg aaagaatatg cgccaggcgt tcgccgcgaa
    65461 tattatgctg ggcgatatat tttttcatcc ctgccagcgc cagcgcgcca gagggttccg
    65521 ccaccgcgcg cacatcttcg aacaggtctt tcatcgccgc acatatggca tcgctatcga
    65581 cggtgatgat gtcgtcgaga tactcctggc ataaacggaa ggtttcgtcg ccgatgcgtt
    65641 tcaccgcaac accttcggca aacagcccca cgcgtggcag atcaaccgga tgacctgcat
    65701 ccagcgccgc tttcaggcag gcggagtcct ccgcttccac ggcgatcact ttgatttgcg
    65761 gcatcagttg tttgatcagt accgccacgc ccgccgccag accgccgccg ccgacgggta
    65821 caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc cagcgccagc gtgccttgcc
    65881 cggcgatcac catcggatgg tcgaacggcg gcacccaggt gaagccctgc tgctgtgaca
    65941 gttcgatcgc tttcgctttt gcttcatcaa agttcgcgcc gtgaagaagc acttcgccgc
    66001 cgaagccgcg caccgcgtcg actttgatat cggcggtggc ggttggcatg acgatcagtg
    66061 ccttcacgcc taaccgtgcg gaagaaaacg cgacgccctg cgcgtggtta cccgcagaag
    66121 cggtgatcac gccgtgcgct ttctgttctt ccgtcaggcc cgccatcatg gcgtatgcgc
    66181 cgcgcagctt aaagctatga actggctgac ggtcttcgcg tttcaccaga atcacgttat
    66241 cgagacgcga cgagagtttt tccatttttt gtagcggcgt gacctgcgcc gcttcgtaga
    66301 ccggcgcgcg cagcaccgct cttaaatatt cggcaccttc aggggcaccg gacaggggtt
    66361 gtgagtctgc catcattaac cccccagttt cgatttatcg cgcaccgcac ctttgtcggc
    66421 gctggttgcc aggctggcgt aggcacgcag cgcaaaggaa acctgacgtt cacggttttt
    66481 cggcgtccag gctttgtcac cccgagcttc ctgcgcttca cgacgcgccg ccagttcggc
    66541 atcgcttacc tgtaactgaa tgccacggtt cggaatgtcg atagcgataa gatcgccgtc
    66601 ttcaatcagg ccaatgctgc cgccgcttgc cgcttccggt gagacgtggc cgatagaaag
    66661 accggacgtg ccgccggaga agcgtccgtc ggtgatcagc gcacaggctt taccgagacc
    66721 cattgatttc aggaaactgg ttgggtagag catttcctgc atccccggac cgcctttcgg
    66781 gccttcatag cgaattacta ccacatctcc ggcgacaact ttaccgccga gaatcgcttc
    66841 taccgcgtcg tcctggcttt cgtagacttt cgccgggcca gtgaatttga ggatgctgtc
    66901 atcgacgcct gcggttttaa cgatgcagcc gttttctgcg aaattaccgt agagcaccgc
    66961 caggccgccg tctttgctgt aggcgtgttc cagcgagcgg atacagccat tggagcgttc
    67021 gtcgtccagc gtatcccagc ggcaatcctg cgagaatgcc tgtgtagtac gaatgcccgc
    67081 cgggccagcg cggaacatat tttttaccgc atcatcctgg gtcacgatga tgtcgtattg
    67141 ttccagcgtt tgcggcaacg tcaggccaag tacatttttc acatcacggt tcagtaaccc
    67201 cgcacgatcc agctcgccga gaataccgat aacaccacca gcacggtgaa catcttccat
    67261 atggtatttt tgggtgctcg gcgcaacttt acacagctgc ggtactttgc gggagagctt
    67321 atcgatatca ctcatggtga agtcgatttc cgcttcctgc gccgccgcca gcaggtgaag
    67381 tacggtgttg gtagagccgc ccattgcgat atccaacgtc atggcgtttt caaacgccgc
    67441 tttgctagca atattgcgcg gcagtgcact ttcgtcattt tgctcgtagt aacgtttggt
    67501 caattcaaca atgcgtttac cggcattgag gaacagctgc ttacgatcgg cgtgagttgc
    67561 caacagcgag ccgttgcccg gctgcgacag gcccagcgct tcggtcaggc agttcattga
    67621 gttagcagta aacatcccgg agcaggaacc gcaggttgga caggcggaac gttcaacctg
    67681 atcgctctgg gagtcagaaa ctttcgggtc tgcgccctgg atcatcgcat caaccagatc
    67741 gagcttaatg atctgatcgg acagtttggt tttcccggct tccatcgggc cgccggaaac
    67801 aaagatcacc ggaatattca ggcgcaggga agccatcagc atccccgggg tgattttgtc
    67861 gcagttagag atgcagacca tggcgtcggc gcagtgggcg ttgaccatat actcaacgga
    67921 atcagcgatc agttcgcgag atggcagtga ataaagcatc cccccgtggc ccatggcaat
    67981 cccatcatcc accgcaatgg tgttgaactc tttggcaacg ccgccagccg cttcaatttg
    68041 ttcggcgacc agtttaccga gatcgcgcag atggacgtga cccggtacaa attgggtgaa
    68101 cgagttcaca accgcgataa tcggcttacc gaaatcggcg tcggtcattc cggtggcgcg
    68161 ccacagcgca cgagcacccg ccatattacg accatgggtg gtggtggcgg aacggtactt
    68221 aggcatactt tatttactcc cagtgtctgt ctcgtaaatg ggacggtgcg tgccgtccca
    68281 ttttttgtat ttattgatta acttgatcta accagcccca tttatcttcg gtttcgccag
    68341 tgaagaggcc gaagaaggct tgctgaatgc gtttggtgac cgggccgcaa cggccttcgc
    68401 caacctgaat accgtctacg ctgcgcactg gcgtgatttc tgccgccgta ccggacataa
    68461 acacttcatc cgccaggtac agggattcgc gcgacagcac ctgctcacgc acttcaattc
    68521 ccagctcttt tgccagtttg atgatggcat cacgggtaat acccggcagc gcggatgagg
    68581 tgaacggtgg ggtgaacagc acgccgtctt tcacttcaaa caggttttcg cctgcgcctt
    68641 cagagatgta accattcaca tccaacgcga taccttcctg ataaccgtgg cggcgcgctt
    68701 cgctacccac cagcagggaa gagaggtagt taccaccggc ttttgccgcc gtcgggatgg
    68761 tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc gatcccctgc tccagcgctt
    68821 ctgcgcccag atacgctccc cacgggaaag cggcgataat cacgtcggtt gagtatcccg
    68881 ctggcgggtt tacgcccatg ccaacatcac caacgaagat cagcggacgg atataggcgc
    68941 tggtgagatt gtttttgcgg atcacgtcac gacaagcttc catcagctca tcaatgctct
    69001 gcgaaaccgg gaagcgatag attttggcgg agtcatgcag acgctgcata tgctcacgat
    69061 ggcggaatac aaccggtcct ttgtgcgagt cgtagcaacg gatgccttca aaaaccgagg
    69121 tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc gtcttcccag cgaaccatct
    69181 ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat ttttatattc cttttgcgct
    69241 caggcgcgga tttgttgtga tgtggttgtg ctctggcaga tagcaacgtg tgcgacgtcc
    69301 accagtttat ttaactgact aaacagtaag tcgaccgacc gtgggctggc aacggtcaat
    69361 tcgatattta tattttgtgc atcgctggcg gcggccatat tcattgagca gacgtggaaa
    69421 ccacgatgac gcaccacgcg taaaacacgt tctaaggttt ccggattgaa gcgagccgat
    69481 acattgacct gatgttgcat catgataatt tctccaacat ttcagaatta ctggcaccag
    69541 gcggcaccag cggccagacg ttctcaagtt cgtcgattga gacatgaagc aggtatggcc
    69601 catcactgtt cagcatggtg tcgagtgccg cttcaacctg gtctttacgg gtgatgtgtt
    69661 ggccagggat gccgaaggcg ctggctaaca tgaggaaatc ggggttatcg gtaagggttg
    69721 tttcgctgta tcgttcctgg aaaaacagtt gctgccattg tcgaaccatc cctaaccgtt
    69781 ggttatcgag taagacgatt ttcaacggta actgcttgcg ttttacggtg cccagctctt
    69841 gcacattcat catgaaagag ccgtcaccgg agatacagac gacggtatcg ttcggtcgcg
    69901 cgacttgtgc gccaaccgcc gccggtaaac caaaacccat ggtgcctaag ccgctggagg
    69961 tgatgaaatt ttccgggcga gtgtgggcga tgtgctgcgc agcccacatc tggtgctgcc
    70021 ccacatctgt ggtcacgacg caatccgcag gttttcgatc cgacagttgt tttaacaaca
    70081 acggcgcgta gatagcgtca ccgggatggt cgtaacgcca ggtatgttca tcacgcagct
    70141 gcgcgcagta ttgctgccag tcattgatat ttaacggctg ctgtaatgct ggtaacagag
    70201 catttaaatc accctgtaat gccacatgtg cctgacgcag cttgttcatt tctgccgggt
    70261 cgatatccat atggataacg ctggcgtgtg gtgcgaaggt gttcagtttg ccagtcaccc
    70321 ggtcatcaaa acgtgcgccc acggcgatca gtagatcgca ctcctgcact gcgaagttcg
    70381 ccgctttggt gccgtgcatc cccagcatgc ccagatagta cggataatct gcttctactg
    70441 cgcccagccc tttcaacgta caggtggcag gcatttttgt ggcagcgaga aattcacgca
    70501 aagccgaaac tgcctgcgcc atgcccacgc caccgccaac gtacagcatc ggtttttgcg
    70561 cttttgccag catctgacgc gcttgctcga cttcggcatg tgggaaagtc acttcgtttt
    70621 caacagtggt gaaccacggt tccagatcgc cgctggctaa ctggatatct tttggaatat
    70681 cgaccaaaac cggaccagga cgaccagagc tggcgacgtc aaacgcttcg gccatgatac
    70741 gcggtaactc ttccagtgat tgcaccagga aactgtgctt ggtgcaggct aacgacaatc
    70801 ccaggacatc cacttcctga aatgcgtcgg tgccgataaa cgctgcggat acctgaccgg
    70861 tgatagcaac aacggggatg gaatctaaca gcgcgtccgc aagcccggtt atcaggttgg
    70921 ttgcacctgg accagacgtg gcgatgcaaa cgccagtttt gccggtagca cgggcataac
    70981 cgatagccgc cattgctgca ccttgctcgt gtcggcacag caagtgttcc acgccaccgt
    71041 catacaatgc atcgtaaacc ggcataattg cgccacccgg ataaccgaaa acggtattca
    71101 caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc attcatagtt agttccccgt
    71161 cctgaatctt gagaaacaga attttgtgct gttattcatt gtctgctcct cggttatgtt
    71221 tttaaggtca aaaaaaaccc ccggaccttt cggtgcgggg gtcttagttc gttaaggctt
    71281 gatctctatg cctttcctcg tccaagtgca gccccgcacg gtgggataat aatcaccacc
    71341 acgctaatca cgaccaggct aatcactcgt agaagggctg tcattttgtc ttttcttgca
    71401 tcttgttcga aggaatgcct aaagagttac catagatttt gcgaatagca caagatattt
    71461 tttggccgat ttttatgatg tctttaataa tttcaagaga aattgttgtt ttttaaaaag
    71521 aaaatagtga gtgaaaaaaa ttcaatttca gtatgaatgt tgttcccttc ctcgtagttc
    71581 atcctttgcc cgctgcattt ttgcgagcat cattccaccg ccagaacaac tcaacttcac
    71641 ttgcacaaat aaacaggaat cagccactaa aattcacgaa ttagcgggct acagcagcga
    71701 tatcttatgc acataatggt gactcaagga gggcttatgt cactgtcaat tgttcatacc
    71761 cgcgcagccc tgggagtaaa tgcgccccca atcactgttg aggtacatat cagtaaaggt
    71821 ctaccaggct taacgatggt gggcttacca gaaacaacgg taaaagaagc tcgcgatcgc
    71881 gtgcgcagcg ccattatcaa tagcggatat gaatatccgg cgaaaaaaat caccatcaac
    71941 cttgctccag ccgatctgcc aaaagaaggg ggacgatatg atttacctat cgccattgcg
    72001 ttgctggcag cctcagaaca gcttacagcc aataagttag atgaatatga attagtcgga
    72061 gaactggcgc ttacaggcgc tctgcgtggc gttcccggcg caatctccag tgcaactgaa
    72121 gctattaagt cgggcagaaa aattatcgtc gcgaaagata acgaagatga agtggggcta
    72181 attaacggtg aaggatgcct gatagccgat catctgcagg ctgtctgcgc gtttctggaa
    72241 ggtaagcacg ctctcgaaca cccgaaacca actaatgcag tatcccgggc gctacaacat
    72301 gatctcagtg atgttgtcgg tcaggagcaa ggaaagcgag gactggaaat taccgccgct
    72361 ggcgggcaca accttttact gattgggccg ccgggaacag gtaaaacaat gctcgccagc
    72421 cgtattaatg gcctgttgcc agatttaagc aatgaagagg cactggagag cgctgcgata
    72481 ttaagtctgg taaatgctga atcagtacaa aaacaatggc ggcagcgccc attccgctca
    72541 cctcatcaca gtgcgtcgtt aactgcgatg gtgggcggtg gtgcaattcc agggccaggt
    72601 gaaatttcgc tggcgcataa cggcgtgctt tttcttgatg agctacctga atttgaacgg
    72661 cgtacactgg atgccttgcg agagccgatt gaatccgggc agatccatct ttcacgcaca
    72721 cgagcaaaaa taacctatcc agcccgtttc cagcttgttg cggcgatgaa tcccagccct
    72781 accggacatt atcagggaaa ccataaccgc tgcacaccag aacagacatt acgttatctc
    72841 aaccggctct cggggccctt tctcgaccgc ttcgatctct cactagagat cccattaccg
    72901 ccccccggca ttttgagtaa aacggtagtg cagggagaaa acagcaccac cgttaaacaa
    72961 cgtgtaatgg ccgccagaga gcgccaattt aagcggcaga ataaactgaa cgcctggctg
    73021 gataatccgg aaatacgcca attctgcaag cttgagagcg aagatgcgca gtggctggaa
    73081 gaaacgctga tccatctggg gttatcgatt cgtgcctggc agcggttatt gaaagttgca
    73141 cgaaccattg ctgatattga tcagtctgac attatcacac gtcagcattt gcaggaggca
    73201 gttagctatc gtgcgattga ccgtttgctc atccatctgc aaaaactact gacataaaaa
    73261 aagggcattt cgcccttttt attaatcgtc agaatcggtg tagtcttcag caccttcaac
    73321 ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga cgcttgatac gcgtcatata
    73381 cttggaccac acgcgttctg cttctgtcac tggttcacgt tcgccacggc atactgctac
    73441 gaagagtttc tcttcctcgg taaccggctc gcgtttgcca agatccaact cattgaaggc
    73501 ataaccatga cgctcaagca gttgtgcctc tttgatggtg aagtcaccat gacgagagaa
    73561 tccacgtgga taatgtttat tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat
    73621 cctgcacgct cctaattctt tgaccgagct agttatggcg cggagtatta gttacgcttg
    73681 acagagtgta aaacaaaaca tttaaatcat aacgacaaat aattttgcgg agagcactgt
    73741 ggatacggaa ttgttaaaaa ctttcctgga agttagccga acgcgtcact ttggtcgagc
    73801 ggctgaatcg ctctatctga cccaatcagc cgtgagcttt cgaatcagac aactggaaaa
    73861 tcaactgggt gtgaatcttt tcacccgcca cagaaacaat atccgtttaa ccgctgccgg
    73921 agaaaagctt ctgccttatg cagaaacgct catgagcacc tggcaggccg cccgcaagga
    73981 ggtggcgcat acctcacgac ataatgagtt ttctatcggt gccagcgcct cgctgtggga
    74041 gtgtatgctt aatcagtggc tgggacgctt gtatcaaaat caggatgtcc atacaggctt
    74101 acagttcgaa gcgcgaattg cccaacggca gtctctggta aaacagttgc atgaacgcca
    74161 gcttgatctt cttattacaa ctgaagcccc caaaatggac gaattttgta gtcagttgct
    74221 ggggtatttc actttagcgc tttataccag tgccccttct aaactaaagg gagatcttaa
    74281 ctatctgcga ctagagtggg ggccagattt tcaacagcat gaggcaggtt tgatcggtgc
    74341 tgacgaagtt cccattctga caaccagttc tgctgaactg gcacagcaac agattgcgat
    74401 gcttaatggt tgcacctggc tacccgtcag ttgggcacgt aaaaaaggcg gcctgcatac
    74461 cgttgtcgat agcacaacac tttcacggcc gctttatgcc atatggctac aaaatagcga
    74521 taaaaatgcg ttgattcgcg atctattgaa aattaacgtg ctggatgaag tgtattaatc
    74581 tgaatggctg gcaaggatgc cggtagaagg atttacttcg gagagggtta tttcagataa
    74641 aaaaaatcct tagctttcgc taaggatgat ttctggcagg ggcggagaga ctcgaactcc
    74701 caacacccgg ttttggagac cggtgctcta ccaattgaac tacgccccta attagggtgg
    74761 cggaacggac gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac
    74821 tgaactaccg ctccaccgaa ttcttttaca accaccggtt tataaccggc ttac
//
LOCUS       DAZKHK010000006        67812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKHK010000006 DAZKHK010000000
VERSION     DAZKHK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.006"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..71
                     /locus_tag="ACHOS5_000390"
     CDS             <1..71
                     /locus_tag="ACHOS5_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775278.1"
                     /translation="KSKIRGIRKYNAKSQVKLVYFK"
     gene            complement(145..1347)
                     /gene="ubiI"
                     /locus_tag="ACHOS5_000391"
                     /gene_synonym="visC"
     CDS             complement(145..1347)
                     /gene="ubiI"
                     /locus_tag="ACHOS5_000391"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP9775279.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRACCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1370..2548)
                     /gene="ubiH"
                     /locus_tag="ACHOS5_000392"
                     /gene_synonym="visB"
     CDS             complement(1370..2548)
                     /gene="ubiH"
                     /locus_tag="ACHOS5_000392"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP9775280.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITSVHVSDRGHAGFVTLTAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGGVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQRDREATIGVTDSLVHLFANRWGPLVVGRNIGLMAMELFTPARDVLAQRTLGWVAS
                     "
     gene            complement(2545..3870)
                     /gene="pepP"
                     /locus_tag="ACHOS5_000393"
     CDS             complement(2545..3870)
                     /gene="pepP"
                     /locus_tag="ACHOS5_000393"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP9775281.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLINGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAATRKQ"
     gene            complement(3896..4474)
                     /gene="ygfB"
                     /locus_tag="ACHOS5_000394"
     CDS             complement(3896..4474)
                     /gene="ygfB"
                     /locus_tag="ACHOS5_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP9775282.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4642..4971
                     /gene="zapA"
                     /locus_tag="ACHOS5_000395"
     CDS             4642..4971
                     /gene="zapA"
                     /locus_tag="ACHOS5_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9775283.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            5013..5196
                     /gene="ssrS"
                     /locus_tag="ACHOS5_000396"
     ncRNA           5013..5196
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOS5_000396"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5217..5819
                     /gene="fau"
                     /locus_tag="ACHOS5_000397"
     CDS             5217..5819
                     /gene="fau"
                     /locus_tag="ACHOS5_000397"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9775284.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHMVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5921..5980)
                     /gene="ibsC"
                     /locus_tag="ACHOS5_000398"
     CDS             complement(5921..5980)
                     /gene="ibsC"
                     /locus_tag="ACHOS5_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP9775285.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(6209..7441)
                     /gene="serA"
                     /locus_tag="ACHOS5_000399"
     CDS             complement(6209..7441)
                     /gene="serA"
                     /locus_tag="ACHOS5_000399"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9775286.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(7697..8356)
                     /gene="rpiA"
                     /locus_tag="ACHOS5_000400"
     CDS             complement(7697..8356)
                     /gene="rpiA"
                     /locus_tag="ACHOS5_000400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9775287.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8412..8642)
                     /locus_tag="ACHOS5_000401"
     CDS             complement(8412..8642)
                     /locus_tag="ACHOS5_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9775288.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            8783..9676
                     /gene="argP"
                     /locus_tag="ACHOS5_000402"
     CDS             8783..9676
                     /gene="argP"
                     /locus_tag="ACHOS5_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP9775289.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            9879..10973
                     /locus_tag="ACHOS5_000403"
     CDS             9879..10973
                     /locus_tag="ACHOS5_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HFP9775290.1"
                     /translation="MSNVLVWQQLANNELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDFDNPRVAGDVGKAGVAIDTVEDMKELFDQIPLDKMSVSMTMNDAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKQSMRIIADIIAWCS
                     GNMPRFNNISISGYHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYL
                     ENASGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(10970..11866)
                     /gene="ygfI"
                     /locus_tag="ACHOS5_000404"
                     /pseudo
     CDS             complement(10970..11866)
                     /gene="ygfI"
                     /locus_tag="ACHOS5_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(12024..12764)
                     /gene="yggE"
                     /locus_tag="ACHOS5_000405"
     CDS             complement(12024..12764)
                     /gene="yggE"
                     /locus_tag="ACHOS5_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP9775291.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            complement(12857..13492)
                     /gene="argO"
                     /locus_tag="ACHOS5_000406"
     CDS             complement(12857..13492)
                     /gene="argO"
                     /locus_tag="ACHOS5_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP9775292.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(13631..14491)
                     /gene="mscS"
                     /locus_tag="ACHOS5_000407"
     CDS             complement(13631..14491)
                     /gene="mscS"
                     /locus_tag="ACHOS5_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9775293.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(14682..15761)
                     /gene="fbaA"
                     /locus_tag="ACHOS5_000408"
     CDS             complement(14682..15761)
                     /gene="fbaA"
                     /locus_tag="ACHOS5_000408"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9775294.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(15976..17139)
                     /gene="pgk"
                     /locus_tag="ACHOS5_000409"
     CDS             complement(15976..17139)
                     /gene="pgk"
                     /locus_tag="ACHOS5_000409"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9775295.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAVELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(17189..18208)
                     /gene="epd"
                     /locus_tag="ACHOS5_000410"
     CDS             complement(17189..18208)
                     /gene="epd"
                     /locus_tag="ACHOS5_000410"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP9775296.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            18580..19011
                     /locus_tag="ACHOS5_000411"
     CDS             18580..19011
                     /locus_tag="ACHOS5_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP9775297.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            19034..19612
                     /locus_tag="ACHOS5_000412"
     CDS             19034..19612
                     /locus_tag="ACHOS5_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9775298.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            19613..20320
                     /locus_tag="ACHOS5_000413"
     CDS             19613..20320
                     /locus_tag="ACHOS5_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9775299.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     TMILLMLAEFGAWIWLR"
     gene            20308..20985
                     /locus_tag="ACHOS5_000414"
     CDS             20308..20985
                     /locus_tag="ACHOS5_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9775300.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPKAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            20979..21656
                     /locus_tag="ACHOS5_000415"
     CDS             20979..21656
                     /locus_tag="ACHOS5_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9775301.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(21628..22341)
                     /locus_tag="ACHOS5_000416"
     CDS             complement(21628..22341)
                     /locus_tag="ACHOS5_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP9775302.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(22338..22847)
                     /gene="fumE"
                     /locus_tag="ACHOS5_000417"
     CDS             complement(22338..22847)
                     /gene="fumE"
                     /locus_tag="ACHOS5_000417"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP9775303.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKIAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(22869..23184)
                     /locus_tag="ACHOS5_000418"
                     /pseudo
     CDS             complement(22869..23184)
                     /locus_tag="ACHOS5_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(23181..24458)
                     /gene="yggP"
                     /locus_tag="ACHOS5_000419"
     CDS             complement(23181..24458)
                     /gene="yggP"
                     /locus_tag="ACHOS5_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9775304.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QMLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LAWLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(24473..25861)
                     /gene="cmtA"
                     /locus_tag="ACHOS5_000420"
     CDS             complement(24473..25861)
                     /gene="cmtA"
                     /locus_tag="ACHOS5_000420"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9775305.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(25889..26332)
                     /gene="cmtB"
                     /locus_tag="ACHOS5_000421"
     CDS             complement(25889..26332)
                     /gene="cmtB"
                     /locus_tag="ACHOS5_000421"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP9775306.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMVSLLNKNYISENYIQ
                     AIKGSTINNGPYYILAPGVAMPHARSECGALKTGMSLTLLKQSVYFPGNDEPIKLLIG
                     LSAADANSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            26710..27618
                     /locus_tag="ACHOS5_000422"
     CDS             26710..27618
                     /locus_tag="ACHOS5_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFP9775307.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(27837..29828)
                     /gene="tkt"
                     /locus_tag="ACHOS5_000423"
     CDS             complement(27837..29828)
                     /gene="tkt"
                     /locus_tag="ACHOS5_000423"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9775308.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            30106..30864
                     /gene="loiP"
                     /locus_tag="ACHOS5_000424"
                     /gene_synonym="yggG"
     CDS             30106..30864
                     /gene="loiP"
                     /locus_tag="ACHOS5_000424"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP9775309.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYARRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(31068..31988)
                     /gene="speB"
                     /locus_tag="ACHOS5_000425"
     CDS             complement(31068..31988)
                     /gene="speB"
                     /locus_tag="ACHOS5_000425"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP9775310.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(32124..32855)
                     /locus_tag="ACHOS5_000426"
     CDS             complement(32124..32855)
                     /locus_tag="ACHOS5_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775311.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTDVIGFAAFELQGPAK
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(33001..34977)
                     /gene="speA"
                     /locus_tag="ACHOS5_000427"
     CDS             complement(33001..34977)
                     /gene="speA"
                     /locus_tag="ACHOS5_000427"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP9775312.1"
                     /translation="MSDDMSMGLPSSAGEHSVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(34986..35117)
                     /gene="yqgB"
                     /locus_tag="ACHOS5_000428"
     CDS             complement(34986..35117)
                     /gene="yqgB"
                     /locus_tag="ACHOS5_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP9775313.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(35211..35354)
                     /gene="yqgG"
                     /locus_tag="ACHOS5_000429"
     CDS             complement(35211..35354)
                     /gene="yqgG"
                     /locus_tag="ACHOS5_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP9775314.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(35465..35716)
                     /gene="yqgD"
                     /locus_tag="ACHOS5_000430"
     CDS             complement(35465..35716)
                     /gene="yqgD"
                     /locus_tag="ACHOS5_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP9775315.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTQFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            35772..36926
                     /gene="metK"
                     /locus_tag="ACHOS5_000431"
     CDS             35772..36926
                     /gene="metK"
                     /locus_tag="ACHOS5_000431"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9775316.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            37362..38756
                     /gene="galP"
                     /locus_tag="ACHOS5_000432"
     CDS             37362..38756
                     /gene="galP"
                     /locus_tag="ACHOS5_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP9775317.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            38833..39330
                     /gene="yggI"
                     /locus_tag="ACHOS5_000433"
     CDS             38833..39330
                     /gene="yggI"
                     /locus_tag="ACHOS5_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP9775318.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            39425..40132
                     /gene="endA"
                     /locus_tag="ACHOS5_000434"
     CDS             39425..40132
                     /gene="endA"
                     /locus_tag="ACHOS5_000434"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP9775319.1"
                     /translation="MYRYLSIAAVVLSTAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            40212..40943
                     /gene="rsmE"
                     /locus_tag="ACHOS5_000435"
     CDS             40212..40943
                     /gene="rsmE"
                     /locus_tag="ACHOS5_000435"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9775320.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            40956..41906
                     /gene="gshB"
                     /locus_tag="ACHOS5_000436"
     CDS             40956..41906
                     /gene="gshB"
                     /locus_tag="ACHOS5_000436"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP9775321.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            42015..42578
                     /gene="yqgE"
                     /locus_tag="ACHOS5_000437"
     CDS             42015..42578
                     /gene="yqgE"
                     /locus_tag="ACHOS5_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP9775322.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            42578..42994
                     /gene="ruvX"
                     /locus_tag="ACHOS5_000438"
     CDS             42578..42994
                     /gene="ruvX"
                     /locus_tag="ACHOS5_000438"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9775323.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(43168..44148)
                     /gene="yggR"
                     /locus_tag="ACHOS5_000439"
     CDS             complement(43168..44148)
                     /gene="yggR"
                     /locus_tag="ACHOS5_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP9775324.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            44166..44870
                     /gene="yggS"
                     /locus_tag="ACHOS5_000440"
     CDS             44166..44870
                     /gene="yggS"
                     /locus_tag="ACHOS5_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP9775325.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPTSAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            44888..45454
                     /gene="yggT"
                     /locus_tag="ACHOS5_000441"
     CDS             44888..45454
                     /gene="yggT"
                     /locus_tag="ACHOS5_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP9775326.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            45451..45741
                     /gene="yggU"
                     /locus_tag="ACHOS5_000442"
     CDS             45451..45741
                     /gene="yggU"
                     /locus_tag="ACHOS5_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP9775327.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            45749..46342
                     /gene="rdgB"
                     /locus_tag="ACHOS5_000443"
     CDS             45749..46342
                     /gene="rdgB"
                     /locus_tag="ACHOS5_000443"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP9775328.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            46335..47471
                     /gene="hemW"
                     /locus_tag="ACHOS5_000444"
     CDS             46335..47471
                     /gene="hemW"
                     /locus_tag="ACHOS5_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9775329.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLNGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYLQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(47536..48543)
                     /gene="yggM"
                     /locus_tag="ACHOS5_000445"
     CDS             complement(47536..48543)
                     /gene="yggM"
                     /locus_tag="ACHOS5_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP9775330.1"
                     /translation="MKKQWIVGTALFMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVTILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(48660..49706)
                     /gene="ansB"
                     /locus_tag="ACHOS5_000446"
     CDS             complement(48660..49706)
                     /gene="ansB"
                     /locus_tag="ACHOS5_000446"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP9775331.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPVRK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(49882..50601)
                     /gene="yggN"
                     /locus_tag="ACHOS5_000447"
     CDS             complement(49882..50601)
                     /gene="yggN"
                     /locus_tag="ACHOS5_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP9775332.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQMKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(50785..51111)
                     /gene="yggL"
                     /locus_tag="ACHOS5_000448"
     CDS             complement(50785..51111)
                     /gene="yggL"
                     /locus_tag="ACHOS5_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP9775333.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            complement(51111..51830)
                     /gene="trmB"
                     /locus_tag="ACHOS5_000449"
     CDS             complement(51111..51830)
                     /gene="trmB"
                     /locus_tag="ACHOS5_000449"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9775334.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            51991..53043
                     /gene="mutY"
                     /locus_tag="ACHOS5_000450"
     CDS             51991..53043
                     /gene="mutY"
                     /locus_tag="ACHOS5_000450"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9775335.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLASAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQISADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            53071..53346
                     /gene="yggX"
                     /locus_tag="ACHOS5_000451"
     CDS             53071..53346
                     /gene="yggX"
                     /locus_tag="ACHOS5_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP9775336.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPENKK"
     gene            53411..54490
                     /gene="mltC"
                     /locus_tag="ACHOS5_000452"
     CDS             53411..54490
                     /gene="mltC"
                     /locus_tag="ACHOS5_000452"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP9775337.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQATNIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            54692..55948
                     /gene="nupG"
                     /locus_tag="ACHOS5_000453"
     CDS             54692..55948
                     /gene="nupG"
                     /locus_tag="ACHOS5_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP9775338.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(55997..58132)
                     /gene="speC"
                     /locus_tag="ACHOS5_000454"
     CDS             complement(55997..58132)
                     /gene="speC"
                     /locus_tag="ACHOS5_000454"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP9775339.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     SHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(58295..58378)
                     /locus_tag="ACHOS5_000455"
     CDS             complement(58295..58378)
                     /locus_tag="ACHOS5_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775340.1"
                     /translation="MHATSLHSLAYRRFARNSSLFMLLLMR"
     gene            58531..59238
                     /gene="yqgA"
                     /locus_tag="ACHOS5_000456"
     CDS             58531..59238
                     /gene="yqgA"
                     /locus_tag="ACHOS5_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9775341.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            59344..59419
                     /locus_tag="ACHOS5_000457"
     tRNA            59344..59419
                     /locus_tag="ACHOS5_000457"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59377..59379,aa:Phe,seq:gaa)
     gene            complement(59615..60652)
                     /locus_tag="ACHOS5_000458"
     CDS             complement(59615..60652)
                     /locus_tag="ACHOS5_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9775342.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(60720..62201)
                     /locus_tag="ACHOS5_000459"
     CDS             complement(60720..62201)
                     /locus_tag="ACHOS5_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFP9775343.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESRIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLRDGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(62194..63684)
                     /locus_tag="ACHOS5_000460"
     CDS             complement(62194..63684)
                     /locus_tag="ACHOS5_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9775344.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIGTALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYQYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGYRGTGYDISGLVQENGHA"
     gene            63916..65280
                     /locus_tag="ACHOS5_000461"
     CDS             63916..65280
                     /locus_tag="ACHOS5_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9775345.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDNHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DATALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAGDTATLA
                     AARTHSDENNKRTLISANTYADQKETDINNRTDGLLMTERLARIAGDAETLATAEAYS
                     DAGDSLTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAI
                     SSIPYVAENSFSYGIGVGNYQYANAVAAGIQYKTSRNTNIRLNISLDSSHNTAIGAGV
                     AGGW"
     gene            complement(65520..66008)
                     /locus_tag="ACHOS5_000462"
     CDS             complement(65520..66008)
                     /locus_tag="ACHOS5_000462"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9775346.1"
                     /translation="MFQLTPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AVDNDHHIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GALLADALYRAYTSEIAAYALIVDAKDDTAVAFYKHYGFMAFPESGRTLFMPLASLST
                     IK"
     gene            complement(66014..66295)
                     /locus_tag="ACHOS5_000463"
     CDS             complement(66014..66295)
                     /locus_tag="ACHOS5_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9775347.1"
                     /translation="MPSVTQTARLEARISTELHAMLKRAAEIQGRTMTDFIISAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNMLYTK"
     gene            complement(66712..66984)
                     /locus_tag="ACHOS5_000464"
                     /pseudo
     CDS             complement(66712..66984)
                     /locus_tag="ACHOS5_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
     gene            complement(67410..67652)
                     /locus_tag="ACHOS5_000465"
     CDS             complement(67410..67652)
                     /locus_tag="ACHOS5_000465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775348.1"
                     /translation="MMMARDITAYHLNSVHISARRFPEIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
BASE COUNT        16733 a        18078 c        16565 g        16436 t
ORIGIN      
        1 ctaaatcaaa aattcgtggc attagaaaat ataatgcgaa atcgcaggtg aaattagttt
       61 atttcaaatg aggaaaatct cccggcgaaa aaaccgggag atgaaagtgt gatgggtatc
      121 aaataaacag cagaagagaa atatttaacg cagccattca ggcaaatcgt ttaatcccat
      181 tgcctggcgg ataagttgcg gcttaacgcc aggaagcgta tcagccagtt tcagaccaat
      241 atcgcgcagc agttttttcg ccggattagc cccggaaaac agatcgcgga atccctgcat
      301 accagccagc atcagcgccg cgctgtgctt gcggctacgc tcatagcgac gaaggtaaat
      361 gtactgcccg atgtctttac cctgacgatg caaccgtttc agttcagcaa tcagctctgc
      421 agcatccata aagccgagat ttaccccctg ccccgccagc gggtgaatgg tatgcgcggc
      481 gtcgcccacc aacaccagac ggtgcgcggc aaactggcgc gcataacgcc ccgtcagcgg
      541 gaacacctga cgctcactct caaccttgca taagcccagg cgattatcaa aagcgatatt
      601 taacgcgcga ttaaattcgt cttcgcctgc ctgctgcatc cgctgcgctt cctctggcga
      661 cagtgaccag acaatcgagc aaagatgcgg atcgctaagc ggtaaaaagg ccagaatgcc
      721 ttcgccatgg aaaacctgcc gcgccaccgc atcatgcggt tcttccgtgc gaatggtcgc
      781 caccagcgcg tgatgctgat aatcccagaa agtcagcgga atatcggctt tgttgcgcaa
      841 ccaggaatta gcgccgtccg cgccaatcac cagacgcgcc gttaacatgc tgccatcttt
      901 caacgtcagg aaggtttcat tttctcccca ggcgacctgc tgtaattctg cgggggccaa
      961 cagagtgata tctgacgact gctgggcttt gttccacagc gcgtagtgaa tcactgaatt
     1021 ttcaacgata tgcccaagat ggctatagcc catgctttga tcgtcaaacg agatgtgacc
     1081 aaaactgtct ttatcccaca cttccatacc gtgatagcaa caggccctac gagagagaat
     1141 gtcctgccag acgccaagac gggtgagtaa tttttcgctg gcggcattga tagccgaaac
     1201 gcgcagttgt ggtggtgcat ccgccgccag aggttccggt acgcgctgct ccagtacggc
     1261 aacgcgtaag ccgctaccct gtaagccgca ggcaaccgcc agcccgacca tgccgccgcc
     1321 aacaatggct acatcaacac tttgcattgt ttattcctta aaaccgcctt caactcgcca
     1381 cccaaccaag agtgcgctgc gccagcacat cgcgtgccgg ggtgaataat tccattgcca
     1441 tcagcccgat gttgcgcccg acaaccagcg gcccccaacg gttggcaaaa agatgtacaa
     1501 ggctgtccgt gacgccgatg gttgcttcgc gatcgcgctg tcgacgctgc tgataacggc
     1561 acaatacgcc gtaatcaccc atgtcttctc cgcgctcctg cgcctgagtc agagtttctg
     1621 caaggctcat cacatcacgc ataccgaggt tgaacccttg cccggcgatg gggtgcagag
     1681 tttgcgccgc attgcccacc agcacggtac gatgggtaat aggtttggcg gcgcgggtta
     1741 atgccagcgg ataagcactg cgtttaccgg cgtgggtaat ttgccccagt cgccagccaa
     1801 aggcagactg gagttcacgg caaaacttct cgtcgctcca cgacagcacc tcttcgcgcc
     1861 gttccagtgg atgacaccag accagcgaac agcgtccgtc agacatcggc aacatcgcca
     1921 gcgggccatg ttgcgtaaag cgttcaaaag cgcgcccttc gtgcgcaacg gaagtcgcaa
     1981 cgttggcaat cacagccagt tgttcgtaag gctcctgctg ccagtcaacg ccgcacacag
     2041 tggctaacgc tgaatgggtg ccatcagcgg ctaccagcac gccgcccgtc agcgtctcgc
     2101 cactctccag cgtcacttca acgtgactct gagtacgggc aacgttagcc acgcgatccg
     2161 ggcaatgcag cgttacgcca ggtgctttgc gcagcaatgc aaacagccgt tgcccgacat
     2221 tatgcaattc gacaacctgc cccagcgccg ccagttggta atcttctgcg gtgagagtga
     2281 caaatccagc gtgaccacga tcgctgacat gcacgctggt gatggcggtt gcgcaatcag
     2341 ccaaagattg ccagacgccg atgcgcgcca gttgctgaca ggtacccgcc gccagcgcta
     2401 tcgctcgtcc atcaaagccc ggatgagcat gtgactctgg cgcggtcgct tcaatcaaat
     2461 gtaccggcag cgccccgtga cttaaccggg aaatagccag cgccagcgtc gcgcccgcca
     2521 tgccgccacc gacgatgatt acgctcattg ctttctcgta gctgccatta acgcttcaat
     2581 ttcttccggc tttttcacca cgctggcggt gaggttttcg ttgccggttt cggtaatcac
     2641 aatatcgtct tcaatacgaa tgccgatacc gcgatattgt tccggcacat ccgcatccgg
     2701 cgcaatatac agccctggct ctacggtcag taccatgccc ggttccagaa tgcgcgagcg
     2761 atcctggcca taaacaccca cgtcatgaac atccagccct aaccagtggc taaggccatg
     2821 cataaagaaa ggacgatggg cgttctgagc gatcagttca tcaacatcac ctttcaggat
     2881 gccgagtttt accaggccgc taaccatgat gcgcaccact tcaccagtga cttcctgcat
     2941 ggaagttccc ggacgataca ggcgcaggct ggtttcaaga gactccagca caatgtcgta
     3001 gatttcacgc tgggcctgag tgaatttgcc gttgaccggg aaggtgcggg taatatcgcc
     3061 agcgtaacct ttgtattcgc aacccgcatc aatcaacacc aggtcaccgt cacgcagttc
     3121 actctcgttt tcggtatagt gcagaatgca accgttttca ccgctgccga caatggtgtt
     3181 ataggaagga tagcgcgcgc cgtggcggtt aaattcgtgg tgaatttcgc cttccaggtg
     3241 gtactcgaac attcccggac ggcatttttc catcgcccgg gtatgcgcca gagcggtgat
     3301 ttcacccgcg cggcgaagta cggcaatctc ttctggtgat ttgaacaggc gcatttcatg
     3361 aacaacagga cgccagtcga tcatcgttgc cggtgcggtg agattttgcc gcgaaccttt
     3421 acgcagtttc tcaagcgcgc tgttcacgat ttcatcagca tatgcatatt cgccctgagc
     3481 gtggtaaacc acatccagac cattaattag ttgataaagt tgctgattga tttcgctgaa
     3541 tgccagtgcg cggtcaacgc ccagtttctc tggcgcggca tcctggccta agcgacgacc
     3601 aaaccagatc tctgccgtca ggtcgcgaac gcggttaaac aaaacgctgt ggttatgagt
     3661 gtcatcgctt ttaatcagca ccagcaccgc ttccggttcg ttaaagccgg tgaagtacca
     3721 gaagtcactg ttctgacgat aggggtattc gctgtcggcg ctacgtgtta cttctggtgc
     3781 agcaaaaatc agcgcggcgc tgccgggttg catttgctcc accagggcct gacggcgacg
     3841 ctgaaactct tgccgggata tctcactcat aacactctcc ttacattttt tgtttttagt
     3901 gtagagtcgg tttttgtact tctggcgcgg tcggttgcgg atgagtaaag gtgtcgtggc
     3961 ataacagcgc ggcaacacga acgtattcga tgatctcttc aagcgacatt tcaagctctt
     4021 cctgatcttc gtcttcgtcg taacccaact gcgcgatgtt acgcagatcg tcgatggctt
     4081 caccggtttc gccggtcact ttgtccagct tcggttgcgt aacgccaaga ccaagcagga
     4141 agtgattgac ccaaccagcc agcgcatcag cccgatcgaa aacgctgaca tcatcgccat
     4201 caggcagata aagctgaaaa aggaagccgt catcctgcag ggcatcgctg gtggcagagt
     4261 gcattttgcg cagtgcctgt gccagctcat gaccgaaagc catgccttcg ttcgtcaggt
     4321 cgtgaagtag cggcagccat gagctgtcat cgttaccgcc acatatcatc ccactgatta
     4381 aaccatgcat ctcagcaggg gtcagacccg tcccttgttg gttcagatac tggttcattt
     4441 cgttgtaacc aggcatttcg ttctgtatag acataagcat tcgtcgtcaa agggaggaat
     4501 attcatgata tgctaccact ttgggccctg gtggaccaga aaagggcttg tctcttctca
     4561 tcagggtagc tatagtgtcg ccccttcgca gaccatgggt ctaaagacga aggcagcgca
     4621 gtcaatcagc aggaaggtgg catgtctgca caacccgtcg atatccaaat ttttggccgt
     4681 tcactgcgtg tgaactgccc gcctgaccaa agggatgcgt tgaatcaggc agcggacgat
     4741 ctgaaccaac ggttgcaaga tctgaaagaa cgcactagag tcacaaatac tgaacagttg
     4801 gtcttcattg ccgcattgaa tatcagctat gagttagcgc aagaaaaagc gaagactcgt
     4861 gactacgcgg caagtatgga acagcgtatt cggatgctgc agcagaccat agaacaagcg
     4921 ttacttgaac aaggtcgcat caccgaaaaa actaaccaaa actttgaatg acacttttcg
     4981 gtttactgtg gtagagtaac cgtgaagaca aaatttctct gagatgttcg caagcgggcc
     5041 agtcccctga gccgatattt cataccacaa gaatgtggcg ctccgcggtt ggtgagcatg
     5101 ctcggtccgt ccgagaagcc ttaaaactgc gacgacacat tcaccttgaa ccaagggttc
     5161 aagggttaca gcctgcggcg gcatctcgga gattcccttc ttatctggca ccagccatga
     5221 cgcaactacc agaactccca ctgacattat cccgacaaga aatccgcaaa atgattcggc
     5281 aacgtcgtcg tacgttaacg ccggaacaac agcaggaaat gggtcaacaa gccgctaccc
     5341 ggatgatgac ttatcctccg gtggtgatgg cacatatggt cgctgtattc ctctcttttg
     5401 atggcgaact cgacacccag ccactcatag aacaactctg gcgcgccggt aagcgcgtat
     5461 atcttccagt tttgcatccc tttagtgccg gtaatttgct gttcctgaac taccatccgc
     5521 aaagcgaact ggtgatgaac aggttgaaga tccatgagcc aaaactggat gtgcgtgacg
     5581 tgctacccct ttcccgatta gacgtgttga tcacaccgct ggtcgccttt gatgagtacg
     5641 gtcagcgcct gggaatgggc ggtggttttt atgatcggac cttacaaaac tggcagcact
     5701 ataaaatgca accggtgggt tatgcgcatg attgtcagtt ggtggaaaaa ctccccgttg
     5761 aagagtggga tatccctctt cctgcggtgg ttacaccgtc gaaagtctgg gaatggtaag
     5821 ggcgatacat cccgcatcgc cctgattgac atcgttgatt ctttgaccta atttagtgag
     5881 taagggtaag ggaggatgac tcctcccctg agactgactg ttaataagct gggaaactta
     5941 tgagtaacag tacaatcagt atgatgacaa gtcgcatcat aacccttctc cttcaagccc
     6001 tcgcttcggt gagggcttta ccgttacagc cccatgctgc cctgccatcg taaatcccca
     6061 ttaaataaac acaacgcatt gatctgactt tgatttattt tctggagcag actcgcaaag
     6121 tagaatgcgc aatgcggcaa cggtgtggag aagggataaa aaaacgggca agtcagtgac
     6181 ctgcccgttg attttcagag aaggggattt agtacagcag acgggcgcga atggtacccg
     6241 gaatagcttt cattgcctgc agcgcttttt cggcaacgtc ttcgtcggct tcaatatcaa
     6301 taacgacata acccatctgg gcggaagttt gcagatattg cgcggcgatg ttgacgccct
     6361 gctcggcaaa aattttgttg agcgcagtca gcacacccgg acggttttcg tggatgtgca
     6421 tcagacgacg accaccgtgc agtggcagcg agacttccgg gaagttcacc gcagagagcg
     6481 ttgaaccatt gtcagaatac ttgatcaatt tacccgcaac ttccaggcca atattctcct
     6541 gcgcttcctg agtcgaaccg ccaatgtgtg gcgtcagaag gacattgtcg aattcacaca
     6601 gcggagaggt aaatggatcg ctattggtcg ccggttccgt cgggaatacg tcgattgccg
     6661 cccccgccag atgtttgctc gccagcgcgt cacacagcgc tggaatatcc accacagtac
     6721 cgcgcgaagc attaatcagc agcgagccgg gtttcattag cgaaatctct ttcgcgccca
     6781 tcatattttt ggtggacgga ttctctggta catgcagact caccacatcg ctcatattca
     6841 gcaggtcaga aagatgctgt acctgagtgg cgttgcccag cggcagtttg ttttcaatat
     6901 cataaaagta aacatacatt cccagcgatt cagccagaat gcccaattgc gtaccaatat
     6961 gaccgtagcc gatgataccc agctttttgc cgcgcgcttc aaaagaaccc gccgccagtt
     7021 tgttccacac gccacgatgc gctttagcat tggcttccgg cacgccgcgc aatagcagca
     7081 gcagttcgcc aatcaccagc tccgcaacag agcgcgtatt tgagaacggt gcgttaaata
     7141 ccgggatccc gcgctttgcc gccgcatcca gatcaacctg gtttgttccg atacagaaac
     7201 agccaatagc gaccagtttt tctgcggcgt tgatcacgtc ttcagtcaga tgggtacggg
     7261 atcgcaggcc gatgaagtgg gcatcgcgga tggattcttt taattgttca tcatccagcg
     7321 cgcctttgtg aaattcgatg ttggtgtaac cagctgcacg aaggctttcc agcgcctttt
     7381 ggtgcacgcc ttctaccagc agaaacttaa tcttgtcttt ctccagcgat acctttgcca
     7441 tttacccaat cctgtctttt gaaatgttgt gtgcggattt gcatccgcct ttcaacatat
     7501 caaaaaataa tattgcggca atatgaacgt ttgcgtcgcg atgttgagga aatatcacgc
     7561 aaaagtattt cttggatgaa tatgctggtt tagaagattt atttagagca atcgacaatt
     7621 gccaggtaaa aacgtgacac atgtcaccaa atttaatgaa gagaattttt ttaacggggg
     7681 aggttccccc gtcagatcat ttcacaatag ttttgacacc gtcaggtgtg ccaatcagcg
     7741 caacgtccgc gccacggtta gcaaacaaac caacagtcac cacaccagga atcgcattta
     7801 tggcgttttc catcgctatc gggtcaagga tttccatgcc gtggacgtcg aggatcacgt
     7861 tgccgttatc ggtcactacg ccctgacggt attccggacg accgcccagt ttcaccagct
     7921 ggcgcgccac tgcactacgt gccatcggga taacttctac tggcagcggg aatttgccca
     7981 gaatatcaac ctgcttggaa gcgtctgcaa tacagataaa tttttccgca accgaagcaa
     8041 tgattttttc acgggtcagc gccgcgccgc cgcctttgat catttgcatg tggccgttga
     8101 tttcatctgc gccatcaacg tagatgccaa ggctgtcgac ttcgttgaga tcaaaaacgt
     8161 gaatgccgag gcttttcagt ttttcagtgg aagcatctga actggaaacg gccccttcaa
     8221 tctggccttt cattgtaccg agagcgtcaa taaagtgtgc ggcggtggaa cctgtaccta
     8281 caccaacaat ggtgccgggc tgaacatact gaagtgccgc ccatcctact gcttttttca
     8341 attcatcctg cgtcatgatc gtttcgcctg tggtatgaaa tttcacacgc attatataca
     8401 aaaaaagcga ttcagacccc gttggcaagc cgcgtggtta actcatccat aaaatatcgc
     8461 gcaatggcgg gcatcccctt tcgccccgca aataaagcat acaacggtct gggtatgccg
     8521 ctccacggtg caaacaggcg caccagttcc ccgttcgcaa gcccctgttt acaggcaaac
     8581 tgaggtaata acgccacgcc attcaacaca accagggcgc gaactcgctg agcgaaatgc
     8641 attagcgcaa atccctgatc ttttaagctg cgcgttaacc actgatacaa atgttacccg
     8701 ccaacggctt cggtatatgc aacccgacac aaaattgtgt catagtgcag gaaaaagcat
     8761 ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac aggcactgga
     8821 tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca ttacacaatc
     8881 agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc tgttagtgcg
     8941 taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ttggcgctac tacgccaggt
     9001 ggagctgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc cgctgctgct
     9061 ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac tggctcctgt
     9121 gttggctgat tcgcccatcc gcctcaactt gcaggtagaa gatgaaaccc gcactcagga
     9181 acgtctgcgt cgcggcgaag tggtcggcgc agtgagtatt caacatcagg cgctgccgag
     9241 ttgtcttgtc gataaactcg gtgcgctcga ctatctgttc gtcagctcaa aaccgtttgc
     9301 cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcgc cagtggtcgc
     9361 gtttgaccat cttgacgata tgcaccaggc ctttttgcag caaaatttcg atctgcctcc
     9421 tggcagcgta ccctgccata tcgttaattc ttcggaagcg ttcgtgcaac ttgctcgtca
     9481 gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagagctgg ccagcggcga
     9541 actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc accgctttgc
     9601 tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg gtcacaaagt
     9661 tcttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc gttatcctca
     9721 cttctttttg tattccttga atcacattac aaaatagaca aatctcaggc ggcaaaaaac
     9781 gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca ccgtaagcgc
     9841 aaataccctc actttgattg cgttttacgg agcaaataat gtctaacgtg ctggtgtggc
     9901 aacagcttgc caacaatgaa ttgagccgtc gggagaaaac tgtcgactcg ctggttcagc
     9961 aaaccgcgga agggatcgcc atcaagccgc tgtataccga agccgatctc gataatctgg
    10021 aggtgacggg tacccttcct ggtttgccac cctacgttcg tggcccgcgt gccactatgt
    10081 ataccgccca accgtggacc atccgtcagt atgctggttt ctcgacagca aaagagtcca
    10141 acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt gcgtttgacc
    10201 ttgccaccca ccgaggctac gacttcgata acccacgcgt ggcgggcgac gtcggcaaag
    10261 cgggcgtcgc tatcgacacc gtggaagata tgaaagagct gttcgaccag atcccgctgg
    10321 ataaaatgtc ggtttcgatg accatgaatg acgcagtgct accagtactg gcgttttata
    10381 tcgtcgccgc agaagagcaa ggtgttacac ccgataaact gaccggcacc attcagaacg
    10441 atatcctcaa agagtatctt tgccgcaaca cctatattta cccaccaaaa cagtcaatgc
    10501 gtattatcgc agacatcatc gcctggtgtt ccggcaacat gccgcgattt aataacatca
    10561 gtatcagcgg ttaccacatg ggtgaagcgg gtgccaactg cgtgcagcag gtagcattta
    10621 cgctcgccga taggattgag tacatcaaag cagcaatctc cgccgggctg aaaattgatg
    10681 acttcgcccc tcgcctgtcg tttttcttcg gcattggcat ggatctgttt atgaacgtcg
    10741 ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt ggcgcacagg
    10801 atccgtataa caacgttatc cgcaccacca ttgatcattg tgcacatccc atgtatcggg
    10861 attatctgca tcgatatctg gaaaatgcgt ccggcggaca tattcaccac gatcttagcc
    10921 acgtcttcga cttacaccgt aatttaattg caaccggatc gatgctgggt taatggtttg
    10981 tcccgtcacg atcttcagca atatgctgaa gcatcgtggc ggtataacga tgatttctga
    11041 ttgaataaag atattcctgc atatacatcg gcctgtgcgg cgcatcaaaa tatttcagcg
    11101 ttgccggatt caccagtcgc caggcaaaat gcgggatcac cgtcagaaaa tgtccccgct
    11161 ccacggcgct gattttagcc ataaaactgt agggacgata aataatggtg ggattgatcc
    11221 cacaaggacg catattcgca tcaatcattg cttcgaaatt ggcccgattc tgaaagcgca
    11281 tttgtagcca gggcagctcc tgaagcagtt cttgctcttg tcgctcttca taacggcgag
    11341 aaacgagaaa gcccaatcgc agcggcggta gttcactgat agtcaggttt tccagttcct
    11401 gaactcgtgc agatacatgc tgtggggaga taataaaatc taactgacgg tcaaaaagat
    11461 tatcgataac gccattttcg ctgaactcaa ctggctgggc ggtcacgcct tcgtacttat
    11521 ctccgagact aatcaactga tcaaaaataa ttgtcggata agtattatct atgcctatca
    11581 caatttctcg ttaacgacgt cctgagttat ggatttcatt atcaatcgca gaaagtcgtt
    11641 gataaaccgg gaacagtttt tgatacagtt cctgtccggc tttattgagg ctgatattat
    11701 tatctttgcg ggtaaatagc gtatagccaa tttgttcttc cagcgcggca atgcttttgc
    11761 caaatcgcga agccgtcata tggatttttt ctgccgcccg ggcaatatta ttggtttgcg
    11821 ctaataaaat gaaattgcgc atctttttcg aaataaaaat gtccatctcc cctctcccgc
    11881 atccttgtcc gcttaaccgt ttcagtcagc ctatccttga tgaaaccgcg agcaaagata
    11941 ggtgattacg tcatggtttt acagaaaaag gaggcaatat cgggtaaagg cattagcccg
    12001 acgaatacgt cgggctacaa gtattaatgt gcagcaggtg ttttagcggg tggttgatcc
    12061 acaggttcta actggaagac tacatcgacc tgatcatcaa actgaatagc ggcctgctcg
    12121 taagtttcct gtgcggacac cggtgcagca tcagctttca tcatccgcac cattgggctg
    12181 ggctgatagt tggaaacatg ataacgcacg ctatataccg gcccgagttt gcgattaaag
    12241 ccattcgcca gttcctgcgc ctgatgaatc gcgttatcaa tcgctgcctt acgcgctttg
    12301 tctttatagg catccggctg cgccacgccc agcgacacag aacgaatttc gttaagaccc
    12361 gcctttagcg cgccgtccag caaggaattc agtttgtcta actgacggag cgtgacttcc
    12421 accgttctca ctgcgcggta gcctttcagg atacttttac catcctgata atcataatct
    12481 ggctgagtgc gtaagttcgc tgagctgata tcttttttcg cgatctgatt gagctcaagg
    12541 aaggaaatgt attgtgcgac gcgctcatct gcctgtttct tggcagtagc ggcatccttc
    12601 gcggccacgt taacttcaat cgcaagagtg gcaatgtctg gcaccgcatc cacgcttgcc
    12661 gtaccggagg tgacaatatg cggtccatcc ggcaattcgt tagcctgcgc tgccatcccg
    12721 ctaataccca ttaatgccgc cagggcgata actttgaact tcacgttagt ctctccgtgt
    12781 taattgactt gcccaaaatt caagacctct ggaggcaagc ttagcgcctc tgttttattt
    12841 ttccatcaga tagcgcctaa ctgaacaagg cttgtgcatg agcaataccg tctctcgcca
    12901 gctgcaaggc aataaaccac ataacacatc ccactaccag attgataatg cgctgtgatt
    12961 ttgccgtgcg caggcgcggt gccagccagg ctgcgagaat agccagacca aagaaccaca
    13021 ggaaagaggc gctaattgtt ccgagcgcaa accaacgttt tggttccaca tcaagttgcc
    13081 cgccaaggct gcccagcaca acaaaagtat ccaggtaaac atgcggattc agccaggtca
    13141 ctgccaacat ggtggcgata attttccatc tgccttgttt cagaacttcg gcactggcta
    13201 actcaatgtt actgctcatt gctgttttaa aagcgccaaa accataccac agcaagaagg
    13261 ctacgccgcc ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctgc
    13321 caccaaaaat ccccgcacaa atcaggacca aatcgctgat agcacaaagt aaggcaatca
    13381 taatgtggta ctgacgacga atgccctgat tcatcacaaa agcattttgt ggaccgagcg
    13441 gtaggatcat agccgcccca agtgcaagac cttgaaagta ataagaaaac acgttggata
    13501 ttccgaatta agtatctgat gcagagacta tatcgcggga ttgttattag cggaaattga
    13561 taatattaat tggcaatcag aaaaactaat aagagagagg ccagagagta ttatctggcc
    13621 tcagcgttga ttacgcagct ttgtcttctt tcacccgctt aaagttcaca tccatttgcg
    13681 ggtacgggaa gctgataccg gcggcatcaa attcacgttt aatacgctcc agcacatccc
    13741 agtacacgtt ttgcagatcg ccgctgttgc tccagacgcg gaccacgaaa ttaatcgacg
    13801 atgcgccaag ttcgttcaga cgcacagtca tttcgcgatc tttcaaaatg cgatcttcag
    13861 actggataat atcggtcagg atctgcttaa cctgatcgat atcggaatca tacgccacgc
    13921 caataataaa ttcgttacgg cgagctggct cgcgggagaa gttaataata ttcccggcaa
    13981 taattttacc gttcggaata acgataattt taccgtctgc agtacgcatg gtggtggaga
    14041 aaatctgcac actcagcaca gtaccggcta cgccgccgag gtcaacatat tctccggcac
    14101 ggaacgggcg gaacatgaca agtaacacgc cagcggccag gttagacagt gacccctgca
    14161 aagccagacc aacagctaag cctgcggcac cgagtacagc aatgactgac gctgtttgta
    14221 cgcccacgcg ccccagtgca gcgattagcg taaaggcgat aattccgtaa cggactaatg
    14281 cagaaagaaa atcagcaaca gtggcatcga ttttacggga gatcatcagg cgattcaccg
    14341 cgttggaaat catccgcgcg ataatcaaac caacgatgat gatcgcgagt gccgccacga
    14401 tgtttactgc ataacttagc agcagcgcct ggttagccac cagccagctt cccgcgccgt
    14461 ttatgctatc gacaacattc aaatcttcca ttcaatattc cttttcaact gactcaaaat
    14521 ggagaaatac actccgccgc cttatgacgg gcagtctgac aagggtaaac aataaagcat
    14581 aattttgcca aatagatcac agatttctca tctgtatcac cttgcttttg gcgaaaaaaa
    14641 gacccgcaga gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg
    14701 ttcagttcct ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgtagc
    14761 catacgcgcg gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttgcccaac
    14821 tggccttgca ggtaagcttc gttttctttg tagtagttca gaacaccttc ccaggttgcc
    14881 cattgggtat cggtatcgat gttcattttc actacgccgt agcttacgga gtctttgatt
    14941 tcctgagcag tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg
    15001 ttgtgtttct tagaaacata ttcctgagag tcgcgcagga tggtcggagt cagaacaacg
    15061 ttacccggct tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg
    15121 ctgattttgc tcagctcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca
    15181 gaagcgtcca tgtggctgtt gtccacgccg tcttcttcac caccagtgca acccagttcg
    15241 atttccagag tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg
    15301 ttctcttgca gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg
    15361 gtagcagcga agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc
    15421 ttcgcgcagt ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctgatga
    15481 acgtgatgcg caccagagat cgcacccagg atagcagcgc cctgcggaac gtcagatttc
    15541 acgcctttac cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct
    15601 ttaactttag cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact
    15661 gctggcagtg cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca
    15721 gtgattacgc caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt
    15781 cgggccttat actcgtcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca
    15841 atcacttact tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa
    15901 ttatttagag tatgaaaaag tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc
    15961 ctgccctgtg attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac
    16021 tttaccttct acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc
    16081 agcaatgccg aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc
    16141 ttcgctgtct gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc
    16201 gaacacgcca accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag
    16261 ttcctgagcg gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac
    16321 agatttcagg gtagccggtg cagtttcaga gaactcggtt gctacgcgaa catcagacgg
    16381 aaccgggatg ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta
    16441 cagggattta cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccgcc
    16501 aacgatcagc tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac
    16561 tttagaacca ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag
    16621 cgcgtccagt tcaactgcca gcagcgggcc tgcgcacgca acatcagcga atttaccgat
    16681 accgtgagta gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc
    16741 acacagtgca gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa
    16801 gcgaacgttt tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc
    16861 tttaaccaga cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag
    16921 agagaattct tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac
    16981 ttttgcgcct tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc
    17041 gctggttact ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt
    17101 cccagcaaga tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc
    17161 taaaagtttt gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg
    17221 tcgtgtcgag cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga
    17281 tcaggtgtgc gccactgacc cgggtttgag tgccatcgac aatggcactg tgcggatcgt
    17341 ggttaaaatc tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac
    17401 cttgtgctgc tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg
    17461 tcacgcttaa atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcctcaa
    17521 agcgatcgtt aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg
    17581 gaatgatcga ctgactggct gcccgagtgc gacgcaggtc aggatggtat gcatcaataa
    17641 cctgttgatc gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg
    17701 catcatctaa caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa
    17761 cgatgcggtg ttccgcacga agttgatcct gattgacgcc ataaacaacg gtcgcgtcga
    17821 gatcgttact gccaggatgt gaaaagagca cttttttagc cccggcggca atgtgtgctt
    17881 caccatgctc gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt
    17941 cacgccaggg gagcgattgc agtgaacgtt catgcaatac acggatggcg tcatcaccaa
    18001 caaaaagttg atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat
    18061 atttcaacaa atgcgccata cccgcagcat ccgccagttc gttgattgcc accacggtaa
    18121 tttccgcacg gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga
    18181 agccatttat cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt
    18241 tgccagacag agtaatccag caaatcgttc ggggaaacct tacctgtcgc aaactgcgac
    18301 tgattggtta attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac
    18361 gtggaataaa aggaatgatt gtccagtcaa agaagacaat tatctgactt acgtcacgtt
    18421 ttcagccccg cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa
    18481 atcttttcgt tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc
    18541 gatattatcc gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca
    18601 tattcttcct ccgcacgttt ttacgcgctg cgcctgctcc ccgggcagga agttctctcc
    18661 caattacgcg ccttcgcgca gcaacaacaa ctccacgccg cgtggattgc aggttgtacc
    18721 ggcagtctga ctgacgtcgc cttacgctat gccgggcaag aaaacactgc cctgctaagc
    18781 ggtaagttcg aagtcatcgc attgaacggc acgctggaac agagtggtga acatcttcat
    18841 ctctgcgttt ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc
    18901 gtgcggacca cgcttgaact ggtgattggc tgcctggagg aactggcgtt tagccgccag
    18961 ctttgcgcgc tttctggtta cgacgagttg catatttccc ccgttaaata actattttca
    19021 gagaggtttt cttatggcgc gtcgccactt ttccagtcag gcactggtgt tgatcgttat
    19081 ctctatcgcc atcaatatga tcggcggaca actggccagc atggttaaat tacctatttt
    19141 tctcgactcc atcggcacac ttattagcgc ggtactgctt ggtccggtga tcgggatgct
    19201 gaccggttta ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt
    19261 tgcgcccgtc gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt
    19321 tcgcaccctg ccaaaagtcg ttgttagcgg cgtgattatt acgcttgcgg taacggtcgt
    19381 cgctgtaccg ctgcgtactg cgctgttcgg cggcgtgaca ggaagcggag ccgatttgtt
    19441 cgttgcctgg atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacggtgat
    19501 aggcgcgaac ctggtcgata aaattctcac agcggtgatt gtctggcttt tactgcgtca
    19561 gctgccgatt cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc
    19621 gtttacatcc ttaacgctgt gggcgctggc ggcctgcacc acgcttatcc tgcccacaca
    19681 gacaatattg ccagtctata gcgcggcgac ctttttctgc cttattgcgc taaaggccac
    19741 gcggcggcgg gcaaaatatg tcgtctggct gatgttctcg ctgggggctg gattgtggct
    19801 ggtgcacggt ggctggctga cagaatggct aagcggcacg ccacgctctc cagaacggtg
    19861 ggcgcatgct attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg
    19921 gatgcagtac gtccccgtgc aacgttttat ccgcgctttg tttgcttctc gcctgccgcc
    19981 gggcgtcgct tatctgtttg ccgggccgtt attggtcgtt gagcagttaa aacggcaatt
    20041 ggcgattatt catgaagcgc agcgcgcccg cggcgtgccg ctggatgagg gctggtatca
    20101 gcgtttacgt gcgatgcccg cgttaattat tccgctaaca cacaatgcgc taaacgatct
    20161 tgctgtccgt ggtgcggcgc tggatatgcg ggcatttcga atcaacaata ggcgcactac
    20221 gttatgggca ccagccgata gcaccctaca acgcgtagca cgttacacca tgatattgct
    20281 aatgctggct gaattcggag cgtggatatg gttacgttag aacagtttcg ctattgcccg
    20341 acacactcaa cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc
    20401 gccatctttg gtgacaacgg cagtggcaaa agtacgctgg cgcagctgat ggcgggctgg
    20461 tatccggact ttctaccagg agagataacc ggcacgggga cgcttctcgg tacgcccatt
    20521 gggcgtctcc cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat
    20581 ttgcagctct ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat
    20641 ctgtgtcttg ctgaaaaaga gattatggcg cggattgacg ccgccctggc cctgaccgag
    20701 tgccagccgt tgcgccatcg tcacccggca acactctccg gtggagagac acagcgtgtg
    20761 gtcatcgcct gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagc
    20821 cgcctgacgc cgaaggcccg cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa
    20881 cgtggttcgc tgattattct ttttgaacgt catcacacac ctttcctgaa ccactgccag
    20941 caagcgtggc aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat
    21001 cttatcgctg gcctggcgcg gcaacggatt gtctttgcga catttctctg caactcaaac
    21061 aaggtgaatg gctggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag
    21121 taatggcagg tcttcttacg cccacagccg gtacggttat gctgcaacaa caagcgatga
    21181 aaaacctgaa aaaccgccag cgcgcggcaa agattggcgt actgtttcag gaagcggaaa
    21241 atcagctttt tcacagcacc gttgctgacg aaatcgcatt tggtttgata ctgcaaaaat
    21301 gcccggcgga tgaaatcact caacgtacca atgctgccct gcaatgctgt cagttagcag
    21361 acactgccag cgcgcatccg ttagatctgc atagtgcgca gcgacggatg gtcgccgtcg
    21421 cctgcctgga agcactctct ccgccactat tgctactgga cgagccgagt cgggattttg
    21481 acgaaaattg gttgagcgtg tttgaaagct ggctggaaaa gtgccgccag cgaggcacga
    21541 gcgtcgtagc aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc
    21601 gactggaaga tggtgtaatc aggaacgtta atccatcaga cgatattcac ccgtagcggt
    21661 catcattaag gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc
    21721 gtcggtacgg tcataaaagg cttctgcgtc tgccagcgat aaccctcccg ccagcttgcg
    21781 ccccaccaga cgctcccgca acgcagatgc gggagctttg ataaagatcg agaaatcaca
    21841 aaattgcgca agctggcacc acttctcgtc gtccaacaac aaccagtttc cttcgacgat
    21901 gacgagtggt gcggtaacgt gcaacacatc ttcaacagga tcatgctttt gtcgatcgta
    21961 ctgcggccac gtacaatccc cttccacaac ccggcacaga ttttccgcca gtttcgcaac
    22021 gttgaatgtc tctggtgcgc ctttgaaggg tcgcaattga tgcacatcca gccagctatt
    22081 gtaatggtga aaaccatcca tcggcagcgt ctgaatagcg ggcagttccg ggtcttgttg
    22141 cgcgagatat tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag
    22201 aaaaaccacc gtacgccgct gcggatttac tgtctgtaag gccgccagca tacgcagcaa
    22261 tggtttatgt acattttcaa tttcatcatc agagtactgc gcctgtacct ttagtccatt
    22321 caccgtcagt tcgattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc
    22381 agcgccgttt tgaccatccc gctaaaacgc gcctctgacc agacggcgaa atcggcgaac
    22441 ttcatcgctt tcaacgcgtc atagagcgtc gcattgcggg ttatgctatt gacgttgctg
    22501 ataaaatccc aggttatgtc atcagcaaat ctgggcgttt catcctgttc gttcagataa
    22561 tgccaaaact gggaaaaatg cgtgatatcg gcgtacagcc atttatccat tttccccagc
    22621 gcataaatca aacgcagcgc aacatcaata tcgtcaagcg ggccgctttg ggcgagtaac
    22681 ggcttcactg catattcgac gatctcttca tcgttatcga caaacagcga cggcagaaac
    22741 tggcgtatcc cctggagcaa aatagaatgc gcaattttca taaatgaaaa taaattgtcc
    22801 tgtgcattca gttgctcaag cacatcatct tctgtcagcg tcgccattgg tcttccgata
    22861 gatcaccatc aatgcaggga gtctattata ttacacgttt ggtccgcgcc gccgatcagt
    22921 aatgtctgcg tccgcacccc attcgccgtc tgttggatac cgttcaccaa ctcgccgccc
    22981 gtcacgcccg tagcgctaaa gaggatatcg ttccccctcg ccgataacca ctcgcccacg
    23041 aaaagcaacg tcgtttaatg cctggcgcat cgcggtgacc gcaaggccat caattttatt
    23101 tttgtcgcca cagccggttt gcggccaggc agccaacgct gcctgttccg taacgcgaaa
    23161 taatggccac gccagggaca tcattgcgcg gcctccccaa tatcaacgcc aaattgtgcc
    23221 agcagatatt tctcggcctg ttcattccag ataccgtgcg ttttctcaac cagccacgcc
    23281 agctctttaa acagcggatc ggttttgcct ttttcggcaa aatcggcaat ggcggtcagc
    23341 ggcatggtca cgccgttata aatgagtttt ttaccgccag ggatatccgg cagattgagc
    23401 acggtttctg gcaccgcatc caggccaccg atatgggtca ccataaacga cggctgtaac
    23461 tgccccgtgg cgctaagggc aatcgcctct ttcatgtcgt ccgtggaacc gccggacgtg
    23521 ccgacaacgt gcgtactgtt gtaatggacg ttgtagaaat taaacggcac tttgaagttt
    23581 ttatccgtcg gaccggcaaa gaagttcagg cagccatctt ccgccagtaa ttcatccgcc
    23641 atctcgacga ctgaaggcac cgctgcatag acaaatacgt catcaaatcc ggcatcatcc
    23701 gtcaacgccc gcagcgtctg gaccggatcg ctcatccctt tagtattcat atacaccagc
    23761 tcaatgcctt tactggccgc cagttccacc ggcagcagca tctgcacctg cgccagccgt
    23821 ttgtcgtcga tatcgactac caccacccgc gacggttgta tgccgccgtt aatggcgtaa
    23881 tcaatagcgc caattcccat cgggcctgca cacgccagta gcgcaatatt gccgccaggt
    23941 ttgacgccca tacgatgctc ataaacatat tgcgtggtgt gataattggc atgataagcg
    24001 ccaataatgc agcacatagg ctctgccagc gacgccgcag caaaataaga gccgtgatag
    24061 ggtaatacgc agcccaaatt aatggcgatt tcgggaataa tcatataggt ggcgttaccg
    24121 ccaaaatatt cgtagctgta gcccgctgaa tacccgcctg gtaaccccat cgccggttgc
    24181 aatacaaaac gctggccttt tttatatttg ccagtgagat ttttacccac ttcgacaata
    24241 accccggcac attcatgccc ggtaatgacc ggatgatttt ctaaatcgtc ggggacgcgt
    24301 ttatgttcac taccgagtaa cgccgctttc caggtggata aacaaacgct gtcagaaatt
    24361 acattcacta acagttcatt atcggtaatt tctggcagct caaattcgcg cagacggaca
    24421 tcccgcttgc cataaatagc agcaactttg gttttcattt ttacctctgt aatcagtgtt
    24481 tatgttcagc ggttaattga ttaaataaag tgtcgagttt tggatcgcca atatagttat
    24541 taatcaatat cagtggttta tccgtcacac gtttcacgcg cccttccaga ctggcgtgag
    24601 taacgacgat atccgcatcc gcaggcacgt tttctatggc gtaatgtttt acttctattg
    24661 ccagccccgc tttttccagg cgtttacgga aggtggttgc gcccatcgca ctggagccca
    24721 tccctgcatc gcaaacaaag gcaatacgct taacacggct taacgagaat gcaccttctt
    24781 gtttcatcgc cttaaccgca ttggctgact gagcaaactc atcttcgctc ttcgtttcca
    24841 ccgttttttc catcttcagt atcagcgaag taatagcaaa ggacaccagg gtacctaccg
    24901 taacaccggc aattgtcgcc aggaacgagc ctttcggcgt cagtgccagg taagcaaaga
    24961 tagaccccgg acttgggcca gccaccagac caccgtccag taagttaaac atccaggtgc
    25021 cagacatacc gcccgcaatc atggcaataa tggtcagcgg cttcatcagc acatacggga
    25081 aatacagttc gtggatccca ccgaggaagt gaataatcat cgccccgggc gcagaacgtt
    25141 tactcatccc tttaccaaac aaggtaaacg ccagcagcag ccccagacca ggacctggat
    25201 tggaggccac catgaagaag atggatttac cgttaaccga agcctgttgc attcccagcg
    25261 gataatagac gccctgatcg atcgcattat taaggaaaag cactttcgcc ggttcattga
    25321 tgactgacag caaaggtaga taacccgcat gtaccagcgc ctcaatacac tctttgacga
    25381 aagtattagc aattaacacc gccgggccga taacttcaaa acccagcaga caaaggagca
    25441 tccccgcgat acctaatgag aagttattga taaccatctc aaaaccagca ggtatgcgtt
    25501 tttccagcga cttgtcgata tatttaatca ccagaccacc gagcggcccc ataatcattg
    25561 agccaaggaa catcgggatt tcagcgccga cgatcacacc aatcgtacca attccgccca
    25621 tgaccgcgcc gcgtttaccg ccgaccagat gaccaccggt agaaccaatc atcacgggca
    25681 ataaataggt aatcatcgga ccaacaattt tggcgaaatg ttcattcggc agccagccgg
    25741 tgggaataaa taatgccgtt ataaatcccc aggcaataaa agcaccgata ttggggatga
    25801 ccattgcagt caaaaagccc ccaaaagcct ggacctttgc acgagcagac ttgttttcca
    25861 taatattatc ctgtagagga gagagtaatt atccgcggct gataatgtcc gctaattgtt
    25921 tttctgatga tgcggttaag agttgttcga gtatttcttc ttcgcacagt aattcactta
    25981 acgcctgaat agcgccaatg tgcgaattgg catcggcagc agaaagcccg atgagtaatt
    26041 taatcggttc gtcattcccc ggaaaataaa cgctttgttt aagtaacgtc aaagacatcc
    26101 cggttttaag cgccccacat tccgatcgcg catgaggcat tgccacgcct ggtgcgagaa
    26161 tataataagg gccattgtta atggtggaac ctttaattgc ctgaatgtaa ttctcgctga
    26221 tatagttttt attcagcaat gataccatcg agaaatcgat agcttcctgc caatcttttg
    26281 ctgaatgtat aactgagatt gatgactctg gaaaataatc aataagccgc ataaattatc
    26341 cttattttat ttttagggta cagtccggcg gtaaaaataa aatacgcgta caaattaacg
    26401 aaagcaattg gacacatgaa aggagaaact ccacgagcaa aaataacaaa atattaaata
    26461 acaacaacat gaaataagct tttaaaaagt caattataaa tctgttaagt aaactacaaa
    26521 atcaacaaat tattcatcac tatatttgtg atacacatct ctattttctt attgaatgtt
    26581 cgaaaacata cttattacgt ttattatttt ttcgctcatc ccctttataa attctgaatt
    26641 atcggctgtt gtctgttttc tcatcacccc atgtgacata ttcctatact ttactcaagg
    26701 aagattcata tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
    26761 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
    26821 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgagccgggg
    26881 ctgggtttta agatcccttt tatggaaagc gtagagaaaa tttccacgcg taatcaggct
    26941 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
    27001 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacacc
    27061 attgaagcat taaaagatcg tctgattgtc cgtcagttgc caactcaact ggagaacgtt
    27121 ttcggacaat ataccgccat tagcgccgta caggatcgta caaaactggt tcaggatctg
    27181 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
    27241 aatattgatt tttctgacgc ttatgaaaag tcgattgaag accgaatgaa agcagaagtc
    27301 gccattgcca cgcgtaagca aaatctggaa acggagaaaa tccaggcgca aatagcggta
    27361 actcaggccc aggcggaagc agacagtaaa ctagcggcag ccaaagcaga agcggaaact
    27421 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
    27481 gaagctatcc gcctgcgcgg tgaagctctg cgggagaatc ccggacttgt tgccctcacc
    27541 actgctgagc gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
    27601 tttatctcta ctaagtaaaa aagggccgct ttcgcggccc ttgagactga tagcaaagac
    27661 aaaactgcct gatgcgctac gcttatcagg cctacatact tcctgcaata tgttgaattt
    27721 gcaaaatctt gtaggacgga taaggcgttc acgccgcatc cggcataaac aacgcgcact
    27781 ttgccaacaa tctgaagggc cgcatttgcg gcccttctca caaagcatct taccgattac
    27841 agcagcgctt ttgctttcgc aacaacgttg tcaacggtga agccgaactc ttcaaacagc
    27901 tgctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcacc gttcaggcca
    27961 acatacttgt accagtagtc agcaatgcca gcttccaccg caacacgagc ggtaaccgct
    28021 ttcggcagta cggattcacg gtaagccgcg tcctgcttgt cgaatgcgtc ggtagacggc
    28081 atggaaacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc ggcaacagcc
    28141 agttcaactt cagaaccggt cgcgatgaag atcagttccg gctgaccagc acagtctttc
    28201 agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc ctgctgcgcc
    28261 aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac gccgtatttc
    28321 cacgctactg cggattcaac ctggtcacac ggacgccatg tagacatgtt cggggtcacg
    28381 cgcagagaag cgatctgttc taccggctgg tgagtcgggc catcttcgcc cagaccgatg
    28441 gagtcgtggg tgtaaaccat cacctgacgc tgtttcatca gcgcagccat acgtacggca
    28501 ttacgtgcgt attccacgaa catcaggaag gttgaggtat acggcaggaa accaccgtgc
    28561 agggagatac cgttggcaat cgcagtcata ccgaattcac gtacaccgta gtggatgtag
    28621 ttacccgcag catcttcgtt gattgcttta gaaccagacc acagggtcag gttagacggc
    28681 gccaggtcag cggagccgcc gaggaattcc ggcaacagcg gcccgaacgc ttcgatagca
    28741 ttctgagacg ctttacggct ggcgatcttc gccggattag cctgaagttt agcgatgaac
    28801 tctttcgctt tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg ggtaaattca
    28861 gcggcttcct gcggataagc tttcgcgtaa gcagcgaatt tctcgttcca tgcggattct
    28921 ttcgcctggc ctgcttcttt cgcatcccac tgagcataga tttcagacgg gatttcgaac
    28981 ggcgcgtatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc gcccagcggc
    29041 gcaccgtggg agtcgtgggt accggctttg ttcggggaac cgaaaccgat gatggttttg
    29101 cacatcagca gggacggttt gtcagtcact gcgcgcgctt cttctactgc gcgtttgatg
    29161 gatgcagcgt catgaccgtc gatatcgcga ataacgtgcc agccgtaagc ttcgaaacgc
    29221 attgcggtgt cgtcggtaaa ccagccttca acgtgaccat cgatagaaat accgttgtca
    29281 tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca aacttcgtgg
    29341 gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg gtcgacgatg
    29401 tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc cataccgact
    29461 gcgttggcaa taccctgacc cagcggaccg gtggtggttt ctacacctgc ggtgtaaccc
    29521 acttccgggt gacccggagt tttagagtgc agctgacgga agtttttcag ttcttccatc
    29581 ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc gtggccgttg
    29641 gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg tttcaggaaa
    29701 tcacgccaca ggacttcggc aatgtcagcc atacccatag gggcacccgg gtgaccggat
    29761 ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag ctctttacgt
    29821 gaggacattt tgactccaga tcggatgatg aagggcacgc ccttaacgac ttgacgacag
    29881 cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc tcgtcatgta
    29941 catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg ttgcgcaatc
    30001 cactcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt cacctgcaaa
    30061 cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa aattcgcgcc
    30121 ttattggtag caatgagcgt ggctacggtg ctgaccggtt gccagaatat ggactccagt
    30181 ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga tgcgcaggtg
    30241 aaaaaactga gcgatcaggc atgccaggag atggacagca aagcgacgat tgcgccagcc
    30301 aatagcgaat atgctagacg tctgacgaca atttctcgcg cgttaggcga taacatcaac
    30361 ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt tgcaatggct
    30421 aacggctgta tccgcgtcta tagcgggctg atggatatga tgacggataa cgaagtcgaa
    30481 gcggtgctcg gtcacgaaat ggggcacgtg gcattaggcc atgtgaagaa aggaatgcag
    30541 gtagcacttg gtacaaacgc aattcgcgtt gcggcggcct ctgcaggcgg aattgtcggc
    30601 agtttgtcac aatcacaact gggagacctg ggcgaaaagc tagtcaattc gcaatttacc
    30661 caacgtcagg aatcagaggc cgatgattac tcctacgatt tgctgcgcca acgcggtatc
    30721 agcccggcag gtcttgccac cagctttgaa aaactggcga aactagaaga aggccgccaa
    30781 agctcaatgt ttgacgacca tcctgcatcc gccgaacgcg cccagcatat tcgcgatcgt
    30841 atgagcgcgg atggagttaa gtaaagccag gtagtgccga acgtaggtcg gataaggcgt
    30901 tcacgccgca tctgacaaga atggcacatt tgttactccg tgcgcattgc cggatgcgat
    30961 gctggcgtat cttatccggc ctacgggtgc cgagtgtagg tcggataagg cgttcacgcc
    31021 gcatccgaca ttaatggcac atcacccgcg cgcctcttat ctagcgctta ctcgcccttt
    31081 ttcgccgcct ggatatacag catctccagc gccagcgtcg ccgcagccag agcggtgatt
    31141 tccgactgat cgtatgccgg agccacctcc actacgtcca tcccaacgat gttgagatct
    31201 ttcaagccgc gtaccagttt aatggcgcga tcggaggtca ggccgccaat taccggcgta
    31261 ccggtgcctg gtgcaaaagc aggatccagg cagtcgatat caaaggtcag gtaaaccggc
    31321 atatcaccca caatctgttt cacctgggcg ataatgtcat ccacgctgcg atcgttcacc
    31381 tggcaggcgt ccagcacggt aaagccgttg tctttatcaa actcggtacg aataccaatc
    31441 tgcacagaat gattcgggtc gatcagacct tctttcggcg cggtatagaa catggtgccg
    31501 tggtcaaatt cacaaccgtt agcgtaggta tcggtgtggg catcaaagtg taccaacgcc
    31561 attttgccga aatgcttcgc atgagcgcgc agcagcggca gcgtaacaaa gtggtcacca
    31621 ccgaaggaga gcatacgctt accggcagcc agcagcttct cggcgtgcgc ctgcagcttc
    31681 tcgctcatct cacgggcatc tccgaaggca tataccagat cgccgcagtc cacgacgttc
    31741 aggcgctcac gcatgtcgaa attccacggg aagcgattgt gttcccaggc cagattcgtc
    31801 gaaacctgac ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga agtggccata
    31861 tcgaacggca cgccagtaat cacccagtca gcatcgctgt catatggctg gaagttcatc
    31921 ggcaggcgta aaaaaccaaa ggcgttggaa accagtgagt tatcgtattg atgacctaag
    31981 gtgctcatgg atggactcct attacaaagt cgatataaaa aaacccttcc gcgtcgttag
    32041 gcccgacgag gaagggttgg atttgtgaaa cacaagtttg tgctaattat cgccttaaat
    32101 gggcgaggca tcaagcgagg ttgctatttc cgacacggtg gcggcgcata gccattgtta
    32161 tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt ttttcgccgg accttgtaat
    32221 tcaaaggcgg caaagccgat tacatcggta ttacgcacat cgccttgcgc cgaattggct
    32281 gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt cggtatgttt atctttgcgg
    32341 aatcctttaa tcgctaacga atctccggca ttcacaatat aaccgttgtt attcagcgaa
    32401 ccttgcttgc cgttcagcac atccaggcca tcaaccgtag caacgatctc gtagttggtg
    32461 ttacgactgt aattacggac atataattga taattcattc cgactctggc atggacaata
    32521 tagcgtggtt cattattgga tgaatcataa acccgcgtaa taggtagaga attaaaatta
    32581 ccatcgcgaa tagcatattc cagttcgtta atacgaatgc tataaacgcg gtcgtagccg
    32641 gaaggaacat tagtcgaata actaataacg atcatctctg ccggatcgcg ccaggctcgt
    32701 tcagcagata cgccctgaac cgttgagtgc acatccccac cccaggcggt gccgacattg
    32761 cctgatgcac tgctattata ctgcgcatta ctggaacaac cagccagcag cacgattaat
    32821 gccaccaacg cgctacgtac tttccatttt ttcataaccg ttccctctgc atttaattaa
    32881 tgcagcgatt gtagggagat tatttaataa gaaaagcagc taaattattt ttaagaatat
    32941 ataaaaagaa agcgggccga tttacgtaaa taaatcgccc cgccagcaga ataatcttaa
    33001 ttattcatct tcaagatagg tataaccgta caaacctgcc tcgaactctt ccaggaactg
    33061 ttgttgcagt tcagcatcaa gatcggtttt cttcacctga tcgcggaact gagttaacag
    33121 cgttttcgga tcgagctgca catattgcag catgtccgcc acggtatcgc cttcgtcaga
    33181 cagttctacc tctacgctac catcagggaa gacgaacacg tcaaccgctt cggtatcacc
    33241 gaacaggttg tgcatgttgc cgaggatctc ctgatatgcg ccgaccataa agaaaccgag
    33301 catcggcgga ttctccggat cgtactccgg cataggcatt gtcgtggcaa taccgtcacc
    33361 atcaatatag tggtcgatag caccgtctga gtcacaggta atatccagca gcacagcgcg
    33421 gcgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
    33481 ccacgcatcc ggcatcgact ggaacagcga gaagttgacg tacattttgt ccgccatacg
    33541 ttcctgcagc tcgtcgataa tcggacgatg agcacggttt tgcggatcca gctgcttttg
    33601 cacttcatgg cacatgctca aataaagctg ctcagcccat gcgcgttctt gcaggctaaa
    33661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
    33721 ttcacgcaga gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
    33781 ttgcagggcg cgcggtgcgt cttctgcagg cgcggtcggc accgtgtatt cgttacgttc
    33841 cacgccgatg atattcgaca ccagcacggt gtgatgcgca gtcaccgcac gacccgattc
    33901 ggtgattacc gtcggatgtg gcagaccatt ttcttcacac gcatcaccaa tcgcccagat
    33961 gatgttgttg gcgtattcat tgaggccgta gtttacggag cagtcagact gagaacgggt
    34021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
    34081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cctgtcgcga tatcgcgaat
    34141 attcgccatc tgcgaaccga ggtggaagtg cagcagttgc aggctgtcga gacgcccggc
    34201 ttcacgcagc gtttcaacca gttgcagtac ttgagtcgcc gccaggccaa acttcgattt
    34261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
    34321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcaa tttctgacat
    34381 cttctcaatg accagataga ccttgtggcc catcttctcg ccaattaatg ccaggcggat
    34441 atattcgcgg tccttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
    34501 cagcaccgcc atcaactcgg ctttggaacc ggcttccagg cccagcggtt cgcccgaatg
    34561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa cgaggaagta
    34621 atcgccgtta tagccgtagg attcccgcgc tcgtttgaac gcggcgttaa tggaacgcaa
    34681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
    34741 gcgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
    34801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
    34861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
    34921 tagtacactg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
    34981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
    35041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
    35101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
    35161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtagctg
    35221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
    35281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
    35341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
    35401 ccaaaactct gttttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
    35461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
    35521 tacgcagaat gccagcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
    35581 acgattcaaa aaaaagtgga aattgggtga agaactgacc taaaatagcc atccagatgt
    35641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
    35701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
    35761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
    35821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
    35881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
    35941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taataccgtt cgcgaaattg
    36001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgccatcg
    36061 gtaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
    36121 cgggcgacca ggggctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
    36181 cacctatcac ttatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
    36241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
    36301 aaatcgttgg cattgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
    36361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
    36421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg tttcgttatc ggtggcccga
    36481 tgggtgactg cggtctgact ggtcgtaaaa tcatcgttga tacctacggc ggcatggcgc
    36541 gtcatggtgg cggtgcgttc tccggtaaag atccatcaaa agtggaccgt tccgcagcct
    36601 acgcagcacg ttatgtcgcg aaaaacattg ttgctgctgg cctggccgat cgttgtgaaa
    36661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacttc catcatggta gaaactttcg
    36721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgaattc ttcgacctgc
    36781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
    36841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
    36901 tgcgtgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
    36961 gctggccttt ttcttttacc tcaccgcagt ggacaggcgt tcacgccgca tccggcaaaa
    37021 aaccgcccgc acaataacat cattcttcct caacacgttt taccgcaaat tatcatcact
    37081 actgaaatcg attacaccaa ccacaccagc caaatatttg taatattttc atattattat
    37141 tcagctttca cagttgttac atttcctttc agtaaagtct taattgcaga taacaacctt
    37201 taatctatga tgatataact caattatttt catgcactta aatcataact aagataaatg
    37261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    37321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    37381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    37441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    37501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    37561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    37621 tcggcgcaat cctgtttgtt gccggttcac tgttctctgc ggctgcgcca aacgttgaag
    37681 tactgattct ttcccgcgtt ctgctggggc tggcggtggg tgtggcctct tataccgcac
    37741 cactgtacct gtcagaaatt gcgccggaaa aaattcgcgg cagtatgatc tcgatgtatc
    37801 agttgatgat cactatcggg atcctcggcg cttatctttc tgataccgcc ttcagctaca
    37861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    37921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    37981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    38041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaatagca
    38101 acttccgccg cgcggtgttc cttggcgtac tattgcaggt aatgcagcaa ttcaccggga
    38161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    38221 ccgagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    38281 caatcggcct tgttgaccgc tggggacgta aaccaacgct aacgctgggc ttcctggtga
    38341 tggctgctgg tatgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    38401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    38461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    38521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    38581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgctgct ctgaacgtac
    38641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    38701 ttgaacgtaa tctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    38761 ctcaggcttc ctcccatcat ggaggaagcc acctcttgca gtcatctttt cttcgctcta
    38821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgt
    38881 cgcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    38941 aaactctctt acacccagcg cggaacctct gccggaacag cctggctgga aagctatgaa
    39001 attcgcctca atcccgtttt gctgttggaa aacagtgaag cctttattga agaggtagtg
    39061 ccacacgaac tggcacattt gctggtatgg aaacatttcg ggcgcgtagc gccacatggc
    39121 aaagagtgga agtggatgat ggaaagcgtg ctgggcgttc ccgcccgtcg tacgcatcag
    39181 ttcgaactgc aatccgtgcg ccgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    39241 cagcttaccg tacgccgtca taatcgcgta gttcgtggcg aagccgtcta tcgctgtgtt
    39301 cactgcggcg aacagctggt tgcgaaataa ccacctgaac tatcaggaac tttcctgatc
    39361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    39421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcacagcat tttccggccc
    39481 ggcattggcc gaaggtatca atagtttttc tcaagcgaaa gccgcggcgg taaaagtcca
    39541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    39601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgaaaa aatgaaaacc gtgccagccg
    39661 cgtagagtgg gaacacgtcg ttcctgcctg gcaattcggt caccagcgcc agtgctggca
    39721 ggacggtgga cgtaaaaatt gcgccaaaga tccggtctat cgcaagatgg aaagcgatat
    39781 gcataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    39841 ccagtggaat ggcggtgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    39901 aaaagttgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    39961 gcgtgaccaa tacaacctga cactctctcg tcagcaaacg cagctgttca acgcatggga
    40021 caagatgtat ccagttaccg actgggagtg cgagcgtgat gaacgcatcg cgaaggtgca
    40081 gggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    40141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    40201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    40261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgt atggggccgg
    40321 gacaggcgct gcaattgttt gacggcagca accaggtctt tgacgccgaa attaccagcg
    40381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcca gatcgacgat cgcgaatctc
    40441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttactatcc
    40501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    40561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    40621 ctgcctgtga gcagtgtggc cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    40681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    40741 acagcatcaa tacgttgccg ttaccggtcg aacgcgtccg cctgctgatt ggcccggaag
    40801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    40861 tgggacctcg cgttttgcgt actgagacaa ctgcgctcac cgccattacc gcgctacaag
    40921 tgcgttttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    40981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    41041 agcgtcgtgg ttacgaactt cactatatgg agatggccga tctctatttg atcaatggtg
    41101 aggctcgcgc tcgtacccgt actttgagcg tcgaacagaa ctacgataaa tggtacgagt
    41161 tcacgggtga gcaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    41221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagataaag
    41281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgattg taacgaaaaa ctgttcactg
    41341 cctggttctc tgacttaaca ccagaaacgc tggtcacacg caataaagcg cagctaaaag
    41401 ctttctggga aaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    41461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    41521 agcatggcac tcgctactgc atggcgcaaa attacctgcc agccattaaa gatggcgaca
    41581 aacgcgtgct ggtagtggat ggcgagccgg tgccgtattg cctggcgcgt attccgcagg
    41641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    41701 aaagtgactg gaaaatcgcc cgtcagattg ggccgacgct gaaagaaaaa gggctgattt
    41761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    41821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    41881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    41941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    42001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    42061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    42121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    42181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    42241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    42301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    42361 gcaccgataa acaaccgtct gacgtattgg tggctctggg ttatgcctcc tgggagaaag
    42421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccagca gatctgaata
    42481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    42541 atattctcac catgcctggt gtggcaggac acgcctgatg agtggaacct tactcgcctt
    42601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcaccgctcg
    42661 ccctttgcct gcaattaaag cgcaggacgg tacgccggac tggaacatta tcgagcgttt
    42721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    42781 cgaacagccg ttaaccgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    42841 tgttgaagta aagctccatg acgagcgtct tagcaccgtg gaggcacgtt ccggtctgtt
    42901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    42961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttactagcgc acagatttct
    43021 gacaatgtgc tcgttgtttc atgttggatg cggcgtaaac gccttatcca acctacagtg
    43081 agtgcgaatt caaagttacg cttcccttgt aggcccgata agcttgcgca tcgggcatat
    43141 ttacaattcg tatcagacac agcaacgtca caagcgccct tctttcaccc gctgctgata
    43201 actctgctga aacgttatca tccccacctg ctgcccggtt tgaataacat gcggtaactg
    43261 gtgggttttc ccttcacgaa tcaaattccc caccgcgggt gtgttaatca gtaattcaaa
    43321 cagcgccacg cgtccttcct gtttatccac ttccagcttt tgtgacaaca ccgcccgtaa
    43381 actacctgcc agttgattac gcacgggatc tttttcctgc gccggaaacg aatccaccag
    43441 tcgctcaact gcctgcgctg cgccgcgcgt gtgtaatgtc gccagcacca gatgcccggt
    43501 ttctgccgcc gtcagcgcca gacggattgt ctcgctatca cgcagctctc cgagcaaaat
    43561 cacatcagga tcttcccgca atgcagcccg caatcccgat gcgaaagtca tacagtgcaa
    43621 accaatctcc cgctgttgga tcaaacatcg ctgactggta tagagatatt ccacaggatc
    43681 ttccagcgtc agaatatgcg catcggcatg ttgattgaga tagccaacca tcgccgccag
    43741 cgtggtagat ttgccgctcc ccgtcgcccc cgtcaccaga atcaggccat tctcgctctt
    43801 gagtaattcc ggcaataccg gtggtgcgcc aagctgttcg agctgcgggc agtgcgaagg
    43861 taacagccgt aacgccagag aaataccttg ccgttgtgcg aacgcactgc cgcgcaatcg
    43921 ctggttttcc gccagcgaca cagcaaaatc cagctgacca ttctccagca atattgcccg
    43981 ctgatcgtca tccagccact cccgcagtag ctcttcgacg tccggcgcgt caaacggcgc
    44041 agcttccatt ctcccgcgaa tacgccatcg tgcgggccag gcgctgcaca ggtgtagatc
    44101 cgagacgtta tgctttacac taagggccac aatttcttcc atattcatac taagatcctc
    44161 ggaaaatgaa cgatattgcg cataacctgg cacaggtccg ggacaaaatc tcagcggctg
    44221 caacgcgttg cggccgttct ccagaagaaa ttacgctgct tgcagtcagt aaaacaaaac
    44281 ctacgagcgc catcgcagaa gccattgatg ccgggcagcg tcaatttggt gaaaactacg
    44341 ttcaggaagg ggtagataaa attcgccact ttcaggaact gggcgtaaca ggattagaat
    44401 ggcattttat tggcccgttg cagtctaata aaagccgcct ggtggcagag catttcgact
    44461 ggtgtcatac catcgaccgt ttgcgcatcg ctacccgcct gaacgaccag cgcccggcag
    44521 aacttccccc tcttaacgta ctgattcaaa ttaacattag tgatgaaaac agtaagtccg
    44581 ggattcaact ggctgaactg gacgagctgg cagctgcggt cgctgaacta ccgcgtttac
    44641 gtctgcgcgg gctgatggca atccctgcgc ctgagtcaga atatgtaagg cagtttgaag
    44701 ttgcacgcca aatggctgta gcatttgccg gactgaaaac gcgctacccg catatcgaca
    44761 cgctctctct gggaatgtcg gacgatatgg aagccgccat tgcggcaggt agcacaatgg
    44821 ttcgtatcgg cactgcaatt tttggtgcgc gtgattactc taaaaaataa ggaattaaag
    44881 gaatgccatg aatacgttga ctttcctgct ttcaacggtc attgagctgt ataccatggt
    44941 gctgttatta cgcatctgga tgcagtgggc tcattgtgat ttttacaacc ccttctcaca
    45001 gtttgtagtg aaggtaacgc agccgattat cgggccactg cgccgcgtta ttccggcaat
    45061 gggacccatt gatagcgcct cgctgctggt tgcctatatt ctcagtttca ttaaagccat
    45121 cgtgctgttt aaagtggtga ccttcctgcc aatcatttgg attgccggtt tattgattct
    45181 gctgaaaacc atcggcctgc tgattttctg ggtcctgctg gtgatggcga ttatgagctg
    45241 ggtaagccag ggtcgtagcc cgattgaata cgtgctgatt cagctggccg atccgctgct
    45301 gcgcccgatt cgccgcttgc taccggcaat gggtgggatt gatttctcgc cgatgatcct
    45361 cgttctgctg ctgtatgtca tcaatatggg tgtcgcagaa gtcttgcagg cgaccggaaa
    45421 tatgctgctg ccggggctgt ggatggcgtt atgagtgccg taacagttaa tgatgacggt
    45481 ctggttttac ggctctatat tcagccaaaa gccagccgtg attctattgt cggtttacat
    45541 ggcgacgagg taaaagtcgc cattaccgcg ccgccggttg acggccaggc caacagccat
    45601 ctggtgaagt ttctcggtaa gcaattccgg gttgccaaaa gccaggtggt gattgaaaaa
    45661 ggcgaacttg gccgccacaa acaaattaaa atcattaatc cgcaacaaat cccgccagaa
    45721 atcgcggcgt taattaatta ggtatcctat gcaaaaagtt gtcctcgcaa ccggcaatgc
    45781 cggtaaagtg cgtgagctgg cgtcgctgct tagcgacttc ggtcttgata tcgttgccca
    45841 aacagacctc ggcgtagatt ccgctgaaga aaccggcctg acctttatcg aaaacgcgat
    45901 tctgaaagcg cgccatgcgg caaaagtgac cggtttaccg gcaattgccg acgactccgg
    45961 tctggcggta gatgcgctgg gtggcgcgcc gggtatttac tccgcgcgtt attccggtga
    46021 agacgcgacc gatcaaaaga atctgcaaaa actgctggaa acaatgaaag acgtaccgga
    46081 cgaccaacgt caggcgcgtt tccactgcgt gctggtatat ctgcgtcacg cggaagatcc
    46141 cactccgctg gtatgccacg gtagctggcc aggcgtgatt actcgtgaac cagcgggcac
    46201 tggtggcttt ggttatgatc caatcttctt cgtaccttcc gaagggaaaa ccgctgccga
    46261 actgacccgc gaagaaaaga gcgccatttc ccaccgtggt caggcgttga aactgctgct
    46321 ggacgcttta cgtaatggtt aaattaccgc cgctgagtct ctacattcac atcccgtggt
    46381 gcgtgcagaa atgcccgtac tgcgatttca actctcacgc gttgaaagga gaagtgccgc
    46441 acgacgatta tgttcagcat ctgcttaacg atctggacaa cgatgtggct tacgctcagg
    46501 gccgtgaagt aaagacaatc tttattggcg gtggtacgcc gagcctgctt tccggcccgg
    46561 cgatgcaaac gctgctgaac ggcgtacgtg cgcgtttgcc gctggcagcg gatgcagaaa
    46621 ttacgatgga agcgaaccct ggtacggttg aagccgatcg ctttgttgat tatcagcgtg
    46681 ctggtgtgaa ccgcatctcc attggcgtac agagttttag cgaagaaaag ctgaaacgac
    46741 ttgggcgcat tcatggcccg caagaagcga aacgagcggc aaatctggcg agcggtttag
    46801 ggttacgtag ctttaacctc gatttgatgc atgggctacc ggatcaatca ctggaagagg
    46861 cgctaggcga tctgcgccag gctattgaac tgaatccgcc gcatctttcc tggtatcaac
    46921 tgaccatcga acccaatacg ctgtttggct cgcgccctcc tgtactgccg gacgatgacg
    46981 cgctgtggga cattttcgaa caggggcatc agttattaac cgcagcgggc tatctgcagt
    47041 atgaaacatc cgcttacgcc aaacccggtt atcagtgcca gcacaatctc aactactggc
    47101 gctttggtga ctacatcggt attggctgcg gcgcgcatgg caaagtgacc ttcccggatg
    47161 ggcgcattct gcgtaccacc aaaacgcgtc atccacgtgg ttttatgcag gggcggtatc
    47221 tggaaagcca gcgtgatgtc gaagccgcag ataaaccatt tgagttcttt atgaatcgct
    47281 tccgtttgct ggaacctgcg ccgcgcgtgg agtttagcca gtatactggc ctttcagaag
    47341 aggttattcg ccctcagtta gacgaggcta tcgctcaggg ttatctcaca gaatgtgcgg
    47401 attactggca gataacggaa catgggaagt tgtttttaaa ttcgctgctg gagctttttc
    47461 tggctgagta aatttgtatt gccggatgcg gcgtgtacgc aacatccggc tacaactctt
    47521 gcgtcatcgc aaaaattact taatcacccc attgcgccgc aattgttcgg tatctttttt
    47581 ccagcgttca ttagcctggt tgatatcgtc aattttcatc aaaatcgtga cttttttact
    47641 gtttatttct gaaactttca tcgttaacgg atattgccct ttttcgagcg ttatccagtc
    47701 atcctgcaat ttacggctaa gtttttgtga ttgcagaaaa gtctgtcgcc gttgttcgtt
    47761 gatatcaaaa tctcgtttct gctgaattgg cacttcataa cactcttcac tctgtttatg
    47821 acatgccgca atcgccggtt gataaacctc tttgtcatat ttaaccgcga acgcctcgct
    47881 atcgttctgt ttattaaaat catcacgctg ttggtgaatt tttttaaagg cgtcttcgcg
    47941 ggtcatttgc ttaccgtttg catcttttcc ccaatatgca tccgctgctt tctgcgccgc
    48001 caccatttgc ttatcaagcg cttttacatc ctttttcagt gcaggaatcg agttattctg
    48061 gttattcaat gcagactcaa gctgactaaa gcgataattg aaatcttcgc tattcacgat
    48121 cagatattta ttattcgttt tgatatcgtc aaccacccgc ccacgatcgc tggctgccgc
    48181 ctgaaaagag taaaagttca ccgaccagcc agacgcgggc gttcctttac tggttaacat
    48241 cgccgagaat ttcaccggtt tatctttcgt ccaggtctgt tcgagcagct cgtaatctgc
    48301 caactgaccg acccaggtat agagatcgtc actggaagag acatcgcctt ccgctgacca
    48361 ggtggcctga ttacccttag catcaagatt ttttaaggtg atgacatcaa gcttgagaat
    48421 gccgtgatac tgctttttga attgatcttt taagatattt tcagttggcg gttcaccatc
    48481 tgcccggaca ttaccagtca tcaacataaa cagcgccgta ccaacaatcc attgcttttt
    48541 cacccgaaaa ccctcgcgcg gaataattta ttccggtgat tatcattagg gtaataaaaa
    48601 ttggcaatgg acgtgagtct gaagtgaata agccccggca cgataccggg gcgaagtgat
    48661 tagtactgat tgaagatctg ctggatctgc tgcggatctt tagtttgcgt cagagccagt
    48721 tgcagcagaa cgcgcgcttt ttgcgggttc aacgtgccag aggcgacgaa gccgtatttc
    48781 gcatcatcca cttcggcatc ctgagtggta gcgcccgtcg gtacgcggga agaacgcact
    48841 actgcagtgc cgtttttcgc agcggttgca agggtgtcaa atacggtttt atacaggttg
    48901 ccgttaccca cgccagcgct aacgatgcca tcatagcccg catctaccag tgctttagcc
    48961 ggaagatcgg atgcgttagc gtagttataa acaatgccga ctttcggcag ttcattcagc
    49021 ttagagacat cgaacggcgt gtcgctggtg tgcttacgta ccggggtacg ttggtagtca
    49081 atcttaccgt tgtgaatgta acccagagga ccgtagttaa cagacttgaa ggtcgctacg
    49141 tcggtggtgt tggttttggt gacatcacgg ccatcaagca cggtgtcgtt catcactacc
    49201 agtacgccac gattagcgga ggctttatca gctgcggtca ctaccgcgtt atacaggttg
    49261 aatggaccgt ctgcgctcat agacgtggac gggcgcattg caccgaccat caccaccggt
    49321 ttgtcgcatt tcacagtcag gtcgaggaag taagcagtct cttccatcgt gtcggtaccg
    49381 tgggtaatga cgaagccgtc agttttatcg cagtcggtgt taattttttt cgccagtgtc
    49441 agccagacat catcgttcat gtcctgggag ccaatattca ctacctgctc gcctttaacg
    49501 ttcgcaatgt ccttcagttg cggcaccgca ttaaccagat tttctacgcc aactttaccc
    49561 gctgtgtagt tagatttggt tgcggagtca ccaccaccgg caatggtccc gccggttgct
    49621 aaaatggtga tattgggtaa tgccaatgct gcaccactaa aacccataac cagtgcggca
    49681 agtgccgtct ttttgaaaaa ctccatttca ttcctccagt tacgtgaacg ctacgcatta
    49741 tcccttagct ttgtatggga aacttgacgt taaacaattt acaacatgaa tatattttgg
    49801 agatctacaa agttagaggc aggtaacaaa acgaagaatt aaacggcata aaaaagtatt
    49861 atgccgtctt aaaatagagg attattttaa attcccgacc agggctttgc ggctatcttc
    49921 cagagtcaca acgcggctac aaacatcttt gccaaactgc tggaaatctt tttcctgctt
    49981 tttccactcg gtttggattg aggattgcag accgcccagg cttcccagca cgttctgtaa
    50041 tgggttaccg ccgcttttca gcaccgcttt cgcgcccatt tcattaatgc tgtcctgtaa
    50101 aattccgccc attgcctgat tcactaattg ctggccttcg gcgcgaacct gatcaatggc
    50161 tttatagtga aacgtcaggc catcgctgcg cgtttcgata atgcggttca tctgctcttt
    50221 catctgcgca tcaagtttgg tcagacggct gcgcattttg ctgctttcgc ccatctcctg
    50281 aacgataatt ttatccagcg caatacgggc tttctcgacg cggcttttcg cgccttcatc
    50341 aatccacggc agggtgctac gtagttcagc ctgataatcc ttcgcctgct cgcgctgggc
    50401 ggcattcagg gaatattgct taccgttata catcacgttg ccgtctggcg tgatcaccag
    50461 attgccgttt tcgcccttca cctgcacggt ttgcgggctg acaatcacat cgtcacgcgg
    50521 cgtgacgctg cactggtagt cggcgtgagc ggtcattgcc gtcactgaaa gtgctgccgc
    50581 cagcagcatt ttgcgcatca tagtcttccc tcaagaacaa atcaggccag catttgctgg
    50641 ccccagattg ataacaaagt gcgcgttttc catgccggat gcggcgtaaa cgccttatcc
    50701 agcctactcg atcgtgcatc tttgtaggcc tgataagcgt agcacatcag gcagttttgc
    50761 atttgtcatc gcccgtatgc tttcttagtc ccaccaaacg tcgaaaagtt cgctggtgcg
    50821 tacctcttcc agtttgcgtt cttccagcca cttacgcaca atcgcctgat gttcttcggt
    50881 gcatttgccg atttcctgca tgcagatcag accttcccag gccagatatc cgctgccgtc
    50941 aaaggccagt ttgttcggtt cgataacctc gttaataaag tcatcaacgg ttttatcaat
    51001 ctgttcttcc gatgtacctt ccgggaatcg ccatgccacc gaaaatccta attcctggaa
    51061 ttcgtcgatg tgcatttttt tacgcagacg acggctacgg ttctttgcca ttatttcacc
    51121 ctctcgaaca ttaagtccca tactccgtga ccaagacgat gaccacgttg ttcaaatttc
    51181 gtcaccggac gtgatgccgg acgcggtacg taatcattgc tctctgacag gtttttataa
    51241 ccgtcaatag aagacatcac ttcaagcata tgttccgcat aaggttccca gtcggtcgcc
    51301 atatggaata tgccccccag ctgcagtttg ctttttacca gctcggcaaa cggcacctga
    51361 acgatacggc gtttattatg gcgcgctttg tgccacgggt cagggaaaaa gagctgcacc
    51421 atgcgcaatg aattgtcagg aatcatttta tgcagcactt caaccgcatc gtgacacatc
    51481 acgcgcaggt tgcttaagcc ctcttcatgc gcagaagaca ggcacgcacc aacgcccggt
    51541 gaatgcactt caatgccgag gaagtcctgc tcggggcgat ctttagccat tgccaccagc
    51601 gacgcgccca tgccaaaacc aatctcaagc gtcaccggcg cttcacggcc aaaaagcgca
    51661 gggaaatcca gcatatcttc gctgaactca acgcccatca ccggccagta gttttccagc
    51721 gcatgttcct ggcctttggt cagtcgcccc tggcggcgca caaaactacg gatacggcgc
    51781 agcgggcggc cgttttcatc aaattccggt gagatgacgt cgtttttcat aaagatttag
    51841 tcgcttgtga aagtgttctg aaaacgggca ttatccaaag ttagttgccg gatgcaagca
    51901 tgataaggcc gtggctgcgg aaagttccgg tttacaccct gccgtcgctg tgctgcaatc
    51961 ttgcccccaa caacagtgaa ttcggtgacc atgcaagcgt cgcaattttc agcccaggtt
    52021 ctggactggt acgataaata cgggcggaaa acgctgccct ggcaaattga caagacgccc
    52081 tacaaagtat ggctctcaga agtgatgttg caacaaactc aggttgcgac cgttatcccc
    52141 tattttgaac gctttatggc gcgcttccca acggtgaccg atctcgccag tgcgccgctc
    52201 gacgaagttc tccacttgtg gaccgggctt ggctattacg cccgcgcgcg caatctgcat
    52261 aaagcggcac aacaagtggc ggccctacac ggcggtaaat tcccggaaac ctttgaggaa
    52321 gttgcagcac tgccgggcgt cgggcgttcc accgcaggcg cgattctctc gctttctctg
    52381 ggtaagcact ttccgattct cgacggtaac gtcaaacgcg tgctggcgcg ctgctatgct
    52441 gtaagcggct ggcccgggaa aaaagaggtc gagaataaac tatggagttt gagcgagcag
    52501 gtgacgcccg cggttggcgt ggaacggttt aatcaggcga tgatggattt gggcgcgatg
    52561 atttgtacgc gctctaagcc gaaatgctcg ctctgtccgc tacaaaacgg atgtattgcc
    52621 gccgccaaca atagctggtc gctttatccg ggcaaaaaac cgaaacagac gctgccagag
    52681 cgcaccggct actttttgct gttacagcac gaagatgaag tattgctggc gcagcgtccg
    52741 ccgagcggat tgtggggcgg tttatactgt ttcccgcagt ttgccgatga agaaagtttg
    52801 cggcagtggc tggcgcaacg acagatttct gccgataacc tgacgcagct gaccgcgttt
    52861 cggcatacct tcagccattt ccacttagat attgtgccta tgtggcttcc cgtgtcgtca
    52921 ttcaccggct gcatggatga agacaatgcg ctctggtata acttagcgca accgccgtca
    52981 gttggcctgg cggctcccgt ggagcgtttg ttacagcagt tacgcactgg cgcgccggtt
    53041 tagcgcgtga gtcgataaag aggatgattt atgagcagaa cgattttttg tactttcctg
    53101 caacgtgaag cagaaggtca ggattttcag ctgtaccccg gcgaactggg aaaacgcatc
    53161 tataacgaga tctccaaaga agcctgggcg cagtggcagc acaaacaaac catgctgatt
    53221 aatgaaaaga aactcaacat gatgaatgcc gagcaccgca agctgctgga gcaggagatg
    53281 gtcaacttcc tgttcgaggg taaagaggtg catatcgagg gctatacgcc ggaaaataaa
    53341 aaataaaaac agtgccggag cacgcctccg gcaacttgca taaaaacaaa cacaacacgc
    53401 acccggaatg atgaaaaaat atctcgcgct ggctttgatt gcgccgttgc tcatctcctg
    53461 ttcgacgacc aaaaaaggcg atacctataa cgaagcctgg gtcaaagata ccaacggttt
    53521 tgatattctg atggggcaat ttgcccacaa tattgagaac atctggggct tcaaagaggt
    53581 ggtgatcgcc ggtcctaagg actacgtgaa atacaccgat caatatcaga cccgcagcca
    53641 catcaacttc gatgacggta cgattactat cgaaaccatc gccgggacag aacctgccgc
    53701 gcatctgcgc cgggcaatta tcaaaacgtt gctgatgggg gacgatccga gttcggtcga
    53761 tctctattcc gacgttgatg atattacgat ttcgaaagaa cctttccttt acggtcaggt
    53821 ggtggacaac accgggcagc cgattcgctg ggaaggtcgc gcgagcaact tcgcggatta
    53881 tctgctgaaa aaccgtctga agagccgcag caacgggctg cgtatcatct acagcgtcac
    53941 cattaacatg gtgccgaacc accttgataa acgtgcgcac aaatatctcg gcatggtccg
    54001 ccaggcgtca cgcaaatatg gcgttgatga gtcgctaatt ctggcaatta tgcagactga
    54061 atcttccttt aacccgtatg cggtcagccg ttccgatgcg ctgggattaa tgcaggtggt
    54121 acaacatact gccgggaaag atgtgttccg ctcgcagggg aaatccggca cgccgagccg
    54181 cagtttcttg tttgatcctg ccagcaatat tgataccggc accgcgtatc tggcgatgct
    54241 gaacaacgtt tatctcggcg gaattgataa cccaacatcg cggcgttatg ccgtcatcac
    54301 cgcctataac ggcggcgcag gcagcgtgct gcgagtcttt tcgaatgata agattcaggc
    54361 taccaatatt attaacacca tgacgccggg cgatgtttat cagacgctga cgacccgcca
    54421 tccctctgcg gaatctcgcc gttatcttta taaagtgaat actgcgcaaa aatcttaccg
    54481 ccgccgataa ttccactaac cgcccctgac gatgttcagg ggtaaaaatg ttatccacat
    54541 cacaatttcg ttttgcaaat tgggaatgtt tgcaattatt tgccacaggt aacaaaaaac
    54601 cagtccgcga agttgataga atcgcatcat ctcgcacggt caaatgtgct ttttcaaaca
    54661 ctcatccgca tcacgatgtg aggaaattaa catgaatctt aagctgcagc tgaaaatcct
    54721 ctcttttctg cagttctgtc tgtggggaag ttggctgacg accctcggct cctatatgtt
    54781 tgttaccctg aagtttgacg gtgcttctat cggcgcagtt tatagctcac tgggtatcgc
    54841 cgcggtcttt atgcctgcgc tgctggggat tgtggccgac aaatggttaa gtgcgaaatg
    54901 ggtctatgcc atttgccaca ccattggcgc tatcacgctg ttcatggcgg cagaggtcac
    54961 tacgccggag gcgatgttcc ttgtgatatt gattaactcg tttgcttata tgccaacgct
    55021 tgggttaatc aacaccatct cttactatcg cctgcaaaat gccgggatgg atatcgttac
    55081 tgacttcccg ccaatccgta tctggggcac catcggcttt atcatggcaa tgtgggtggt
    55141 gagcctgtcc ggcttcgaat taagccacat gcagctgtat attggcgcag cgctttccgc
    55201 cgttctggtt ctgtttaccc tgactctgcc gcatattccg gttgctaaac agcaagcgaa
    55261 tcagagctgg acaaccctgc tgggcctcga tgcattcgcg ctgtttaaaa acaagcgtat
    55321 ggcaatcttc ttcatcttct caatgctgct gggcgcggaa ctgcagatta ccaacatgtt
    55381 cggtaatacc ttcctgcaca gcttcgacaa agatccgatg tttgccagca gcttcatcgt
    55441 gcagcatgcg tcaatcatca tgtcgatttc gcagatctct gaaaccctgt tcattctgac
    55501 catcccgttc ttcttaagcc gctacggtat taagaacgta atgatgatca gtattgtggc
    55561 gtggatcctg cgttttgcac tgtttgctta cggcgatccg acgccgttcg gtactgtact
    55621 gctggtactg tcgatgatcg tttacggttg cgcattcgac ttcttcaaca tctctggttc
    55681 ggtgtttgtc gaaaaagaag ttagcccggc aattcgcgcc agtgcgcagg ggatgttcct
    55741 gatgatgact aacggcttcg gctgtatcct cggcggcatc gtgagcggta aagtggttga
    55801 gatgtacacc caaaacggca ttaccgactg gcagaccgta tggctgattt tcgcaggtta
    55861 ctccgtggtt ctggccttcg cgttcatggc gatgttcaaa tataaacacg ttcgtgtccc
    55921 gacaggtaca cagacggtta gccactaatt acgcaaagaa aaacgggtcg ccagaaggtg
    55981 acccgttttt ttattcttac ttcaacacat aaccgtacaa ccgtttcatg ccatccgcat
    56041 cggtttcgct atagacacct tgcaactccg gcgaaaatcc cggcaacaaa ttcacgcctt
    56101 cttccagcgc aaggaaataa cgttgaaccg ccccacccca gacttccccg ggtaccacgc
    56161 aaagcacgcc aggtggataa ggcaacgccc cttctgccgc aattcgccct tcggcatcac
    56221 gaatccgcac caactccact tcaccgcgaa tataagcgct atgcgcatcc tggggattca
    56281 tcaccactga cgggaaactc tgctggcgga acatcgcttt ttgtaggtct ttgacgtcga
    56341 aactgacata caaatcgtgc atctcctgac acaactggcg cagggtgtag tcgcgatagc
    56401 gcaccggata tttgttataa acgctcggca acacctcagc cagcggcgaa tcatcctcaa
    56461 tatgctgttc aaactgcgcc agcatcgcca ccagttgtgc cagcttctcg tggctttccg
    56521 ccggagttaa taagaacaga atggagttga gatcgcactt ctccggcaca atgccgttct
    56581 cacgcagata gtgcgccaga atcgtcgctg gaacgccaaa atcgctatat tcgccggttt
    56641 cggcatcgat acccggcgtg gtgagtaaca gcttgcacgg atcaacaaaa tactgatccg
    56701 cggcatatcc ttcaaagccg tgccacttcg cccccggctc aaaactgaaa aaacggcggt
    56761 cgctggctaa cactgacgtc ggataatcct gccacaattt gccatcaaca acaggcggga
    56821 taaacgggcg gaacagctta cagcgcgcaa gaatagcctt gcgcgactca atacccaact
    56881 caacgcactc agcccacagc cgacgcccac tctccccttc atgaattttg gcgttaacgt
    56941 ccagggcggc gaacagcgga tagaacgggc tggtagaagc atggagcata aaggcattat
    57001 tcaaacgctt atgcgggcaa aaacgcgcct gtccacggat atggttatct tttttatgga
    57061 tctgcgacgt ctgtgagaat cccgcctgct gtttgtgcac cgactgggtc acaaagatcc
    57121 ccggatcgtt ttcgttaagt tctaacagca gcggcgagct atccgccatc atcgggataa
    57181 actgttcgta accgacccac gcggaatcaa acagaatgta atcacacaga tgcccaacgg
    57241 tatcgatcac ctgacgggcg ttatagacag tgccgtcata ggttcccagc tgaataatcg
    57301 ccaggcgaaa cgggcgcggc aggtcggctt tttctggcgc aacgtcgcga atttgctggc
    57361 gcagatactc ttcattaaaa cagtgcgcat caataccgcc aatgaaacca aacgggttac
    57421 gtgaagcttc cagatagacc ggcgtcgccc ccgcctgaat caacgcgccg tgatgactcg
    57481 acttatggtt gttacggtcg aagagcacca gatcgccacg cgttaacagc gcattcgtca
    57541 ccactttatt cgctgccgat gtgccgttca gcacaaaata ggttttatcg gcatgaaaga
    57601 ctttggctgc aaatttctgc gcatctttcg ccgatccttc atgaataagc agatcgccca
    57661 attttacgtc agcgttacac atatcggcgc gaaagatgtt ctcgccaaag aaatcgtaaa
    57721 aatggcgtcc ggcaggatgc tttttaaaga acgcaccatg ttgatgtcca gggcaagcaa
    57781 aggtgctatt acccatctca acgtactgcg tcagcgtgtc ataaaacggt ggcagcaaat
    57841 tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg ttgccgttga
    57901 ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa aacaccggta
    57961 gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc gcagcggtaa
    58021 tgacgactgc cgcgacgtcc gtaaaatcag tatctcccaa cgccaccacg cggcgatgag
    58081 tagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc ataagcgcaa
    58141 aacccgttta ggggaagtaa gaataccgga caaggtggaa aaccctgccc catgagatat
    58201 gggtcaaact ggtcaccagc tccgaccgcc agacatcagt aaaagcagaa acgctctgat
    58261 tttactgttg tcctgcagtg agcgtgcgtt aacttcaccg catgagcagt agcataaaaa
    58321 gagaagagtt tcgcgcgaaa cggcgataag cgagagaatg taaggaggtg gcgtgcatcg
    58381 ggcaaactcc gtacagagag gagaaaattc gcgcaatcat ggcacctttc gctaaagcgt
    58441 gcaagccagg actttgcgaa caaacaagcc atcgacggcg ccaaaagttc ataataagaa
    58501 aatcaaacaa tacagctaac aggaactctt gtggtcatcg gcccttttat caacgcaagt
    58561 gcagtcttac tgggtggcgt tttcggcgca ctccttagcc aacgcttacc ggaacgtatc
    58621 cgcgtctcca tgacatcaat ttttggtctg gcgtcgctgg ggattggtat tttactggtg
    58681 gtgaaatgcg ccaaccttcc ggcgatggtt ttagcaacct tactcggagc attaatcggc
    58741 gaaatttgtc tgctggaaaa aggcgtcaat acagtggtcg ccaaagcgca taatctgttt
    58801 caccactcgc gtaagaagcc agcgcatgaa tcttttattc agaattttgt cgcgattatt
    58861 gtcctgtttt gcgccagcgg caccggaatc ttcggggcga tgaacgaagg gatgaccggc
    58921 gatccaagta ttttaatcgc caagtcattt cttgatttct ttacggcgat gatcttcgcc
    58981 tgctcgctgg gtattgcggt gtcggtgatt agcattccat tactgatcat ccagttaaca
    59041 ctggcgtggg ctgccgcgct gatattaccg ctgaccacac cgtcgatgat ggcagacttc
    59101 agcgccgtag gcggtttatt gctgctggca accggattac gcatctgtgg cattaaaatg
    59161 ttcccggtgg tcaacatgct tccagcactc cttctggcaa tgccgctttc cgccgcctgg
    59221 accgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt
    59281 ctgcactaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc
    59341 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt cggtggttcg
    59401 attccgcctc cgggcaccat ataccagaac acgctggtta agggatgaga tccttgacgg
    59461 gcggttaata caggatgcgg catcaaaggg tctccgtttg tgtaagttgt ggttacactg
    59521 attgagttcg gtatcgtatc ctcaaacagg gtgtcttttg cagagacgcc ctgttctcgt
    59581 taacactgta tgcttacatt gctgattcag cctgtcaaga tttattcccc cctttgcaaa
    59641 tcttgctcac tgtgcgctca tctgtataca aactcagggc atcttccagt gtctgcgctg
    59701 cagactccat acagacatca acatacccca tggtcgttgt aagactcctg tgtcccagta
    59761 tggatttgac cagatgtaaa ttacgttcag gatatttcat cagtctggtt gccagcgtat
    59821 gtctgaaacg atgtggtgaa acaggaaaac cacattctcg cgaaagtcgc cggaaaaatg
    59881 aacgtacggt ctgatgcgcc cgaacgggac agtactgata tctgtcggca gagatcggct
    59941 cactgaaacg acagacgtca aaaagatagt catcatcacc tgccccgcac tgacgggccc
    60001 gctccagcag tttttccatc gataccttca ggggagccac cagaggaacg ccccattccc
    60061 tgtgagtttt acttccctcc agcttaagat caattatgct ttcatccgga cagacatccc
    60121 tcacacggat atgcttcagt tgattaaagc gcattccggt ataacgcaga acatccagaa
    60181 ctgtcagcca gtaccaggca gggtatatag cattgcgtcg acgactattc accatgttct
    60241 gctcttccgc ttctgcaaac ctctgcacaa ccaggtaaac cctgagcatc tgattgtcac
    60301 tcagtgtctt tttctttttt tcagtactgg caactgatgc ttccaccaac gggttttctg
    60361 tctgaggcag cagtttcatc ttgatcccga atcccataat tgcccgcaag tgggccacct
    60421 tgttattcca ggtcacctcc ctgcgcttct gctctttcag gacataacgc cggtatcgct
    60481 gaacatcacg gacagtcacc tcccccggca aaatatcacc gataaattcc ctgaataccc
    60541 tgacaacttt gcagtagctc cactctgtat ccggccgaag attctttgaa aaaaagtact
    60601 cttccagcag ttgttcccag gtgatttttg tgacggaatc gtcattctgc attatatatt
    60661 ccccttgttt actgtgctcc cgcactctcc ggtcaaattc tgcacatcat cgcctttact
    60721 cacccgaaaa caataagcgg gctgtctccg ggaaccgtaa tattcccgta cagaaacctg
    60781 cccttaacca gatatcccga aaccattttg taacgtccgg aatgttctgc gtcactataa
    60841 agacgggcct gaaaaaaacg ctctcctgaa cgtaccttat ggatacctgc ccgttcaaag
    60901 agcgactgaa cataacgacg atcctgcccg gaaccactct ccttcagaaa acgataaaaa
    60961 atccccgggg cacacagaaa aacatatccg gaaacaacat gaacatttga atcaacgctg
    61021 ttaaccgaaa gtgaaccatc cctcagcccc tcttttaacc atctgataaa ttcactctct
    61081 accctgctat cctctgtcag ggaaaactct gccgtttttt ccactgtttc cgggatacgg
    61141 tttccctgat tttctgttac acatccgctc tcttccggta actcaatacg gctttcttta
    61201 tccggcggca cgcctgacgc tatctgccct ccctcatccg acaaatcagc agccgaaaac
    61261 agcgtaagga gcattccggt atcagcatca gtttcgttcc cctcatccac ctcctgttcc
    61321 gctgtccggc cctcattttc ggcatcctcc actggcgact gtacaaccgg caacggctga
    61381 atgacgcttt ctgctgaaat tgctgctgac tgcagcagta ccggaacaac ctcagccacc
    61441 ggaacttcgg ccacatctcc tgcagcgagg ggactcacga cttcctccat agcttcctgt
    61501 aatactgtac gtatcagcgc catttcagga tgctgatgcc agactgctcc ggcaagattc
    61561 tgtaatgccc ccgggacagt ggcaagccag gctcccgcct ctccgggcag cagttgtccc
    61621 gccatcaggg ctgccagttg ccgtccctcc gttccctggc agggcatctt cctgaaccgg
    61681 aaccggtata cgccctccgg tacagatatc cccggctgcc agcatctgcc gtcggcctgt
    61741 tcaccttcca gttccgccag caacggcagg tgataaaaca gagccgacca gaacaccacc
    61801 atgttccaca cactcccctg cgcggcctgc tcttctgctg tcgctcccgg tggaaacatt
    61861 ctgcctctgg ccagccggac agcatacgtc gtgaactgca gggtcaggtc accaaaacca
    61921 ccggtgtaac cccagcgacc ttctgctgtg gccggcagat tctgactgac agacaacaac
    61981 tgatgcacag gatcaagaca aaggcgctga tacatatcca ccggcatgga actgttttcc
    62041 cataactgtt tcagacacct tttcctgaac ggtgtggata ccagtacatc agccggcaat
    62101 ggtgtaaaat acccctccgg caccgtttcc ccgggaatat ccgtccggac atcagagcat
    62161 tctgtcttca caggaaataa ccttttcagg cgttcaagca tgcccattct cctgaaccag
    62221 acctgagatg tcgtacccgg tcccccggta accttcagtt ttctgctgtt cttctgccag
    62281 ttggtgcatc aggtcattca gtaccagcgt cctgagggcc cgggtgacag ccacatataa
    62341 cagacaggtt tcatcatgac gctcagcctc cgtcatcagc ggatccgtga tatcctgaaa
    62401 atcctcatcc agcattacaa ccggccactc cagccccttg ctccggtgtg ccgttgacac
    62461 cgtgatatct gcatcctgct cattcgttac cgtatactgc cggagaagtt ccagtttttt
    62521 cggtaacgga aaacacaact ccagaagccg aagggactga ttcatctcca catccttcgt
    62581 ggatttcgca atatactgat attcttcaaa attcctgtag tcccggcgca gcctgtctga
    62641 atgcatccgt tccggcatat caacctggaa ccagtagaga tcctcaagtt ccccggtcct
    62701 gtaaccttcg attcccccca cccagtaaac cttttgccct ttcattgccg ccatcagcgc
    62761 agtaccaatc acacccgcga cagtacgact gagtaccgtg taatgcccct gcagatgttc
    62821 acgcgcatgg catttcagca atacctcatc atcaccacca caacctttca cctctcgtgt
    62881 ttcaccttca cgctgcagaa gcagatttgc catacgagcc accgccggac caaaacggaa
    62941 actctgtgtc agccacaacc ggtccgcatt cttcagtgca ggatgggaaa gcgcattatt
    63001 cgcaccccga aaacggtaaa tctgttgata acaatctcca acaaggacca cccgacactt
    63061 ctgccccaga accagagcag tggtcaccgg attcgcatcc tgcgcctcat caaacaggat
    63121 ggtgtcccat cggtgagaca ggtctgcacc ggacaactgg aagagtttca gataagtatc
    63181 gtgcgtcacc gggaacgtcc ccccggggtt gctcatttcg ttccagagca actgcaccgc
    63241 ccgaagcgca tctgctggcg gaacaccccg catctcatcc ttatccggca ggtgatgcag
    63301 gcccggtacc gcatcggcag aacagataaa gtgattcagc cccgtccctg ccacccgtgc
    63361 cagagcccag tatcgtgtat tcagctttct cgccacatca gtaatacgta atcccggaac
    63421 cagacgatga cggtagtgtc ttccaacccg ggcatacgcc agctgatgtg acgttctgca
    63481 ttccacgtta aagggaaatt tacgctctgc ctcatcacgt attgcccggt tataggcaag
    63541 atagagcata cggctgtccg gactggcccg ggcaaactgc accagcgtac tcgtcttccc
    63601 tgtgccggaa aacgcattca caaccagttg ctgcccctgc cagttaataa tggcgtactg
    63661 ttcatctgtg ggattaaaag acatgcattc tcttctccgg aaaaacattt ccgtaatctg
    63721 atgcatcagg ggtatgccca ccacaaataa ctctgtacag caacagaaaa atttccctgg
    63781 tcatatgatg aattaatcat cgccatcaca ccggatgctt ctcatcctgt cctcagaaag
    63841 acaggtggtc tcctgtctgc cgcgcttatc caggataccg ggacctgaaa ggtcataatc
    63901 tggcaggaaa aaataatgtc cgaagtaaaa aaacacgttt cttattcatt aacagttgca
    63961 gctatgctgt cagccgccat cgtctgtcag acagcacatg ctgatgtcaa cacaccgaac
    64021 atcaccggag aaaaaaacaa tacaacaaca cgattctccg gaaatattta cggcagtgaa
    64081 aatactgtca catcagagtc aaacagtctg gttgtgggta acagtaattc cgtggaggga
    64141 gggtataaca acattgttat tggtaattcc tccacggtca aagcttccgg tttgatcaga
    64201 aaagtgggcg aagatattgt gctggatggt tacagcgtca gcctcggtaa tgccaccaga
    64261 attaacggtg attcatctgt tgccataggt ctgacatcgt ccgtaaccgg gaacgactcc
    64321 gtggcactgg gcagtcattc cagtgctaac ggaaacaata tcgtatccgt cggctctgac
    64381 acactgaaac gccgcatcac aaatctggca caggggaaag ataaccatga tgcggtaaat
    64441 ctgatgcaga tgaccagtgc tgttaaccgg gaagctggag aaagaataaa atcaacaaac
    64501 aacctgcaga atcagcttaa taccctctct ggtcaggtca taactgaaac cagggcccga
    64561 acagaaggtg atgcaacagc gctggctgca gccagagccc acagcgatga aaacgacaaa
    64621 agaacactgg tcagtgcgaa tacctatgct gaccagaaag aaaccgccat caataaccgt
    64681 actgacggat tactgacgac cgaacgccag gcacgcaccg cgggcgatac tgcaactctg
    64741 gctgctgcca gaacgcacag cgatgaaaac aacaaaagaa cactgatcag tgcgaatacc
    64801 tatgctgacc agaaagaaac cgacatcaat aaccgtactg acggattact gatgaccgaa
    64861 cgcctggcac gcatcgcagg tgatgcagaa actctggcaa cagcagaggc atacagtgac
    64921 gccggggatt cactgacact gaaacaggca acagcctaca gcgatatcag tacccgccgg
    64981 gcactgtcag aggccaatcg ttataccgac aacaaattca gaaacctgag caataaaatt
    65041 gaacgggcag aaaaaagact taatgcaggc atcgccggag tgacggcaat atcctccatc
    65101 ccctatgtgg ctgaaaactc tttttcatac gggatcggcg tgggtaacta tcagtacgct
    65161 aatgctgtgg ccgcaggcat tcagtataaa acatcacgta ataccaatat acgcctcaac
    65221 atctccttgg actcatctca taacaccgcg attggtgcag gggttgccgg cggctggtaa
    65281 ataatcttgc gtacatacgg cattatggta ttccgtaatc acattaattc agtacgcaac
    65341 accgccccat ccggaacaga ccactggact gagcgggata atcaaggaaa aagggcgcgc
    65401 agtgtggtca gccttcacaa caatccggcg gcagatttcc tgccgccgtg ttgcccgtta
    65461 acatttaact tcgtgggctg acgttgccac aagcaccgta tttccctgat ttattctgtt
    65521 tattttattg tggataatga tgccaatggc atgaataaag ttcttccgga ctccgggaac
    65581 gccataaagc cataatgttt gtaaaatgcg actgccgtgt catcttttgc atcaacaata
    65641 agcgcatatg ctgcaatttc agatgtgtat gcacgatata gcgcatcagc taaaagtgct
    65701 cccccaagcc ccgttccttt tgatgacaga cttactgcca gtcgtcccat acgaatagcc
    65761 ggaatagtgg gatatcgcgg aagctttttt ctcaaatgct caggtaagtc agttaataat
    65821 atactggccg ctgataacgt atagtatccg atgatatgat gatcgttatc tacagcgaca
    65881 aaacaggccg cgatgcgtcg tcgaatatcc tgtgtgacat gctcttttat gtaacggtcc
    65941 agagcatcag agccacataa aaatgaggtt ctgtcatatg tgctgttaaa tggtgtcagt
    66001 tgaaacatta tatttatttt gtgtacagca tattattacg gcgtttaaag gcccgtttaa
    66061 gcgcatcatt cggctctgtt ggtgacagta atgcctgagc aaaaatttcc tgatctgtcc
    66121 gggacagtcg gattatatcg gtttgttcga tagcgcgttg ggccgcatcc tgaacggcag
    66181 aaatgataaa atcggtcata gtgcgcccct ggatttctgc agcgcgcttc agcatggcat
    66241 gtagttctgt gctgatcctt gcttccagtc gggcagtttg tgttacagat ggcatgagtt
    66301 gtacctcctg aatacactgt acggcaaatt gccgtattgt gcaaattaca ctctgacacc
    66361 gggacggcag acaggcattc tgcctgaaac gaaaaatcag attcagacaa aactatccgg
    66421 gggatgttaa cgccacgatc tttgaccatt gcatacagat aaaagctcag attatttacc
    66481 agcagcgaca tccggagtac accgggattt tatctgtgag tgaacatacc cgggcaaaaa
    66541 atcccccgaa ttcaggggag tgtcaaagag gaattatgta tattggttat tataatgagc
    66601 acgtctccgg tattccgcga cgaatgttca cgtggtggca tgaacggccc ggaaacgtgc
    66661 tcttctgatt tttaacgtaa atttacacta aaggcaactc tgatactgcc ttcaccggat
    66721 ataggagaag gccgtcgtga cccgcttcac tccgctcacc ctactggcaa catctgctgc
    66781 cgctcgccct tcctgctctg taaccaggcc cagtaaaaag acttcgctgt tttctgtcac
    66841 caccttgaca ctggatgatt tgacacgggc acttgtaagt aactgagaac gaatttttgt
    66901 cgtgagccag gcatcacctg ccattatgac cggactgacg ggagtcccct gccggatttc
    66961 attatagacc tcctgaacac caattttaca tgtacaggca tcatattaaa tatggaaata
    67021 ataacgaacg aaaaagggac acaatgaata tcaaagcacc agtataaacc aggatgtttt
    67081 gagcaatttc ttactctcct gaatacacac tgtaaatcat cagaaactgc actagccatg
    67141 ctaagtacac tctgctacga actgatgagg aagtgcggta tcaattacct ggacagcctg
    67201 atcaacggag aggtatatct attatctatg taattacagg gggactgata tcagcgaaaa
    67261 agcaggtcag catattcact gcccctggtc tgaactgaaa caacgtcagc cagaaataat
    67321 cctataatgc ctcatataag tggataactt ttcatccgtc gttgacatca tacacataac
    67381 acattgataa cagtcatgaa tacaccgaat tatctttctg actcagcgtt tccagtcagt
    67441 agcatttctt ccggtaaagg catttccgaa acgtcacgtt cgccattaaa tacaaaaata
    67501 ctttctgcgt aatcattctg taacaaagaa ttagttaacg gcagattttt tgtttgcata
    67561 ccggtggccc actgaaaaat ctcggggaaa cgacgggcac tgatgtgtac agaattgagg
    67621 tggtacgcag taatatctct tgccatcatc ataagatggc acatcatatc ccgaacgcaa
    67681 acctggcaca tgatgaacag acggaaggct cagcaggact tctggataat tatacacgat
    67741 agccagttac tgctgacctg gatttattat cataccacat tatttatttc aaaaaataaa
    67801 ttttaaatta tt
//
LOCUS       DAZKHK010000007        66571 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKHK010000007 DAZKHK010000000
VERSION     DAZKHK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.007"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            119..838
                     /gene="sanA"
                     /locus_tag="ACHOS5_000466"
     CDS             119..838
                     /gene="sanA"
                     /locus_tag="ACHOS5_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP9775349.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            841..1080
                     /gene="yeiS"
                     /locus_tag="ACHOS5_000467"
     CDS             841..1080
                     /gene="yeiS"
                     /locus_tag="ACHOS5_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP9775350.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            1274..2512
                     /gene="preT"
                     /locus_tag="ACHOS5_000468"
     CDS             1274..2512
                     /gene="preT"
                     /locus_tag="ACHOS5_000468"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP9775351.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYLPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            2506..3741
                     /gene="preA"
                     /locus_tag="ACHOS5_000469"
     CDS             2506..3741
                     /gene="preA"
                     /locus_tag="ACHOS5_000469"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP9775352.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELHDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(3984..4994)
                     /gene="mglC"
                     /locus_tag="ACHOS5_000470"
     CDS             complement(3984..4994)
                     /gene="mglC"
                     /locus_tag="ACHOS5_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP9775353.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFIALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(5010..6530)
                     /gene="mglA"
                     /locus_tag="ACHOS5_000471"
     CDS             complement(5010..6530)
                     /gene="mglA"
                     /locus_tag="ACHOS5_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP9775354.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(6591..7589)
                     /gene="mglB"
                     /locus_tag="ACHOS5_000472"
     CDS             complement(6591..7589)
                     /gene="mglB"
                     /locus_tag="ACHOS5_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP9775355.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(7869..8909)
                     /gene="galS"
                     /locus_tag="ACHOS5_000473"
     CDS             complement(7869..8909)
                     /gene="galS"
                     /locus_tag="ACHOS5_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP9775356.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIHQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(9051..10208)
                     /gene="yeiB"
                     /locus_tag="ACHOS5_000474"
     CDS             complement(9051..10208)
                     /gene="yeiB"
                     /locus_tag="ACHOS5_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP9775357.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGGISDSQTSRA
                     WTPDTSAILYEKHWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(10225..10893)
                     /gene="folE"
                     /locus_tag="ACHOS5_000475"
     CDS             complement(10225..10893)
                     /gene="folE"
                     /locus_tag="ACHOS5_000475"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP9775358.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11151..11987
                     /gene="yeiG"
                     /locus_tag="ACHOS5_000476"
     CDS             11151..11987
                     /gene="yeiG"
                     /locus_tag="ACHOS5_000476"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9775359.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(12019..14010)
                     /gene="cirA"
                     /locus_tag="ACHOS5_000477"
     CDS             complement(12019..14010)
                     /gene="cirA"
                     /locus_tag="ACHOS5_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP9775360.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGSSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(14302..15771)
                     /gene="lysP"
                     /locus_tag="ACHOS5_000478"
     CDS             complement(14302..15771)
                     /gene="lysP"
                     /locus_tag="ACHOS5_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP9775361.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPMGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(15976..16857)
                     /gene="yieE"
                     /locus_tag="ACHOS5_000479"
     CDS             complement(15976..16857)
                     /gene="yieE"
                     /locus_tag="ACHOS5_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP9775362.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16956..18005
                     /gene="yeiH"
                     /locus_tag="ACHOS5_000480"
     CDS             16956..18005
                     /gene="yeiH"
                     /locus_tag="ACHOS5_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP9775363.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            18079..18936
                     /gene="nfo"
                     /locus_tag="ACHOS5_000481"
     CDS             18079..18936
                     /gene="nfo"
                     /locus_tag="ACHOS5_000481"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9775364.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIVLDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            18940..20028
                     /locus_tag="ACHOS5_000482"
     CDS             18940..20028
                     /locus_tag="ACHOS5_000482"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9775365.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(20131..20196)
                     /locus_tag="ACHOS5_000483"
                     /pseudo
     CDS             complement(20131..20196)
                     /locus_tag="ACHOS5_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20296..21237)
                     /gene="rihB"
                     /locus_tag="ACHOS5_000484"
     CDS             complement(20296..21237)
                     /gene="rihB"
                     /locus_tag="ACHOS5_000484"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP9775366.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKPN"
     gene            21367..22065
                     /gene="yeiL"
                     /locus_tag="ACHOS5_000485"
     CDS             21367..22065
                     /gene="yeiL"
                     /locus_tag="ACHOS5_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP9775367.1"
                     /translation="MKEIHNNDLKQQLISESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(22132..23382)
                     /locus_tag="ACHOS5_000486"
     CDS             complement(22132..23382)
                     /locus_tag="ACHOS5_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9775368.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALTAATLSNLMSATIAGFFIGLA"
     gene            complement(23476..24414)
                     /gene="psuG"
                     /locus_tag="ACHOS5_000487"
     CDS             complement(23476..24414)
                     /gene="psuG"
                     /locus_tag="ACHOS5_000487"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP9775369.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(24402..25343)
                     /gene="psuK"
                     /locus_tag="ACHOS5_000488"
     CDS             complement(24402..25343)
                     /gene="psuK"
                     /locus_tag="ACHOS5_000488"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP9775370.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(25799..27490)
                     /gene="fruA"
                     /locus_tag="ACHOS5_000489"
     CDS             complement(25799..27490)
                     /gene="fruA"
                     /locus_tag="ACHOS5_000489"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP9775371.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(27507..28445)
                     /gene="fruK"
                     /locus_tag="ACHOS5_000490"
     CDS             complement(27507..28445)
                     /gene="fruK"
                     /locus_tag="ACHOS5_000490"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9775372.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(28445..29575)
                     /gene="fruB"
                     /locus_tag="ACHOS5_000491"
     CDS             complement(28445..29575)
                     /gene="fruB"
                     /locus_tag="ACHOS5_000491"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP9775373.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGHVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            29942..31123
                     /gene="setB"
                     /locus_tag="ACHOS5_000492"
     CDS             29942..31123
                     /gene="setB"
                     /locus_tag="ACHOS5_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP9775374.1"
                     /translation="MHNSPAVTSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(31120..31374)
                     /gene="yeiW"
                     /locus_tag="ACHOS5_000493"
     CDS             complement(31120..31374)
                     /gene="yeiW"
                     /locus_tag="ACHOS5_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9775375.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            31529..32101
                     /gene="yeiP"
                     /locus_tag="ACHOS5_000494"
     CDS             31529..32101
                     /gene="yeiP"
                     /locus_tag="ACHOS5_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP9775376.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            32324..33790
                     /gene="yeiQ"
                     /locus_tag="ACHOS5_000495"
     CDS             32324..33790
                     /gene="yeiQ"
                     /locus_tag="ACHOS5_000495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9775377.1"
                     /translation="MNTIASVTLPHHVHSPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAVSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            33908..34894
                     /gene="yeiR"
                     /locus_tag="ACHOS5_000496"
     CDS             33908..34894
                     /gene="yeiR"
                     /locus_tag="ACHOS5_000496"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP9775378.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            34933..35646
                     /gene="lpxT"
                     /locus_tag="ACHOS5_000497"
     CDS             34933..35646
                     /gene="lpxT"
                     /locus_tag="ACHOS5_000497"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP9775379.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            36058..36624
                     /gene="mepS"
                     /locus_tag="ACHOS5_000498"
     CDS             36058..36624
                     /gene="mepS"
                     /locus_tag="ACHOS5_000498"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP9775380.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            36805..38361
                     /gene="pdeN"
                     /locus_tag="ACHOS5_000499"
     CDS             36805..38361
                     /gene="pdeN"
                     /locus_tag="ACHOS5_000499"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9775381.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            38443..40257
                     /gene="yejA"
                     /locus_tag="ACHOS5_000500"
     CDS             38443..40257
                     /gene="yejA"
                     /locus_tag="ACHOS5_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9775382.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYAEDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            40258..41352
                     /gene="yejB"
                     /locus_tag="ACHOS5_000501"
     CDS             40258..41352
                     /gene="yejB"
                     /locus_tag="ACHOS5_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP9775383.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            41352..42377
                     /gene="yejE"
                     /locus_tag="ACHOS5_000502"
     CDS             41352..42377
                     /gene="yejE"
                     /locus_tag="ACHOS5_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9775384.1"
                     /translation="MPRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDS
                     FDPNKAV"
     gene            42379..43968
                     /gene="yejF"
                     /locus_tag="ACHOS5_000503"
     CDS             42379..43968
                     /gene="yejF"
                     /locus_tag="ACHOS5_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP9775385.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIAAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(43972..44316)
                     /gene="yejG"
                     /locus_tag="ACHOS5_000504"
     CDS             complement(43972..44316)
                     /gene="yejG"
                     /locus_tag="ACHOS5_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP9775386.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(44650..45840)
                     /gene="bcr"
                     /locus_tag="ACHOS5_000505"
     CDS             complement(44650..45840)
                     /gene="bcr"
                     /locus_tag="ACHOS5_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP9775387.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(45868..46563)
                     /gene="rsuA"
                     /locus_tag="ACHOS5_000506"
     CDS             complement(45868..46563)
                     /gene="rsuA"
                     /locus_tag="ACHOS5_000506"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP9775388.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            46713..48473
                     /gene="radD"
                     /locus_tag="ACHOS5_000507"
     CDS             46713..48473
                     /gene="radD"
                     /locus_tag="ACHOS5_000507"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9775389.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            48598..48882
                     /gene="rplY"
                     /locus_tag="ACHOS5_000508"
     CDS             48598..48882
                     /gene="rplY"
                     /locus_tag="ACHOS5_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP9775390.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(49021..50028)
                     /gene="yejK"
                     /locus_tag="ACHOS5_000509"
     CDS             complement(49021..50028)
                     /gene="yejK"
                     /locus_tag="ACHOS5_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP9775391.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            50210..50437
                     /gene="yejL"
                     /locus_tag="ACHOS5_000510"
     CDS             50210..50437
                     /gene="yejL"
                     /locus_tag="ACHOS5_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP9775392.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            50457..52217
                     /gene="yejM"
                     /locus_tag="ACHOS5_000511"
     CDS             50457..52217
                     /gene="yejM"
                     /locus_tag="ACHOS5_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP9775393.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            52292..52368
                     /locus_tag="ACHOS5_000512"
     tRNA            52292..52368
                     /locus_tag="ACHOS5_000512"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52326..52328,aa:Pro,seq:ggg)
     gene            complement(52494..53253)
                     /locus_tag="ACHOS5_000513"
                     /pseudo
     CDS             complement(52494..53253)
                     /locus_tag="ACHOS5_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            53573..54220
                     /gene="narP"
                     /locus_tag="ACHOS5_000514"
     CDS             53573..54220
                     /gene="narP"
                     /locus_tag="ACHOS5_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP9775394.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(54255..55307)
                     /gene="ccmH"
                     /locus_tag="ACHOS5_000515"
     CDS             complement(54255..55307)
                     /gene="ccmH"
                     /locus_tag="ACHOS5_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP9775395.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSEPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(55304..55861)
                     /gene="dsbE"
                     /locus_tag="ACHOS5_000516"
     CDS             complement(55304..55861)
                     /gene="dsbE"
                     /locus_tag="ACHOS5_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP9775396.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(55858..57801)
                     /gene="ccmF"
                     /locus_tag="ACHOS5_000517"
     CDS             complement(55858..57801)
                     /gene="ccmF"
                     /locus_tag="ACHOS5_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP9775397.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMVLGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(57798..58277)
                     /gene="ccmE"
                     /locus_tag="ACHOS5_000518"
     CDS             complement(57798..58277)
                     /gene="ccmE"
                     /locus_tag="ACHOS5_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP9775398.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEEGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(58274..58483)
                     /gene="ccmD"
                     /locus_tag="ACHOS5_000519"
     CDS             complement(58274..58483)
                     /gene="ccmD"
                     /locus_tag="ACHOS5_000519"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP9775399.1"
                     /translation="MTPTFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(58480..59217)
                     /gene="ccmC"
                     /locus_tag="ACHOS5_000520"
     CDS             complement(58480..59217)
                     /gene="ccmC"
                     /locus_tag="ACHOS5_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP9775400.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(59259..59921)
                     /gene="ccmB"
                     /locus_tag="ACHOS5_000521"
     CDS             complement(59259..59921)
                     /gene="ccmB"
                     /locus_tag="ACHOS5_000521"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP9775401.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(59918..60541)
                     /gene="ccmA"
                     /locus_tag="ACHOS5_000522"
     CDS             complement(59918..60541)
                     /gene="ccmA"
                     /locus_tag="ACHOS5_000522"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP9775402.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(60554..61156)
                     /gene="napC"
                     /locus_tag="ACHOS5_000523"
     CDS             complement(60554..61156)
                     /gene="napC"
                     /locus_tag="ACHOS5_000523"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP9775403.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(61166..61615)
                     /gene="napB"
                     /locus_tag="ACHOS5_000524"
     CDS             complement(61166..61615)
                     /gene="napB"
                     /locus_tag="ACHOS5_000524"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP9775404.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(61612..62475)
                     /gene="napH"
                     /locus_tag="ACHOS5_000525"
     CDS             complement(61612..62475)
                     /gene="napH"
                     /locus_tag="ACHOS5_000525"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP9775405.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(62462..63157)
                     /gene="napG"
                     /locus_tag="ACHOS5_000526"
     CDS             complement(62462..63157)
                     /gene="napG"
                     /locus_tag="ACHOS5_000526"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP9775406.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(63164..65650)
                     /gene="napA"
                     /locus_tag="ACHOS5_000527"
     CDS             complement(63164..65650)
                     /gene="napA"
                     /locus_tag="ACHOS5_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP9775407.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(65647..65910)
                     /gene="napD"
                     /locus_tag="ACHOS5_000528"
     CDS             complement(65647..65910)
                     /gene="napD"
                     /locus_tag="ACHOS5_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP9775408.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(65900..66394)
                     /gene="napF"
                     /locus_tag="ACHOS5_000529"
     CDS             complement(65900..66394)
                     /gene="napF"
                     /locus_tag="ACHOS5_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP9775409.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAANCPVSAITAEY
                     LHAH"
     gene            66503..>66571
                     /gene="yojO"
                     /locus_tag="ACHOS5_000530"
     CDS             66503..>66571
                     /gene="yojO"
                     /locus_tag="ACHOS5_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP9775410.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKR"
BASE COUNT        16417 a        17738 c        16358 g        16058 t
ORIGIN      
        1 tccggcttgc cagccctttc ctggtgtcga aaatcccggc aaacagtttg ccgtttcttg
       61 cgcaaaacca gcgggtaaag tagcctgatg gaaattttcc ttagatcgag tctcctgcat
      121 gttaaagcgc gtgttcctca gcctgttagt cctgatcggc ttgctgctgt tgactgtgct
      181 cggcctcgat cgctggatga gctggaaaac cgcgccttat atctacgacg aattgcagga
      241 tctcccctac cgccaggtcg gtgtggtgct cggcacagca aaatattatc ggactggcgt
      301 aattaatcag tattatcgct accgcattca aggagcgatt aatgcctata acagcggtaa
      361 ggtaaattat ctattactga gcggcgataa cgcattgcaa agttataatg agccgatgac
      421 catgcgcaaa gatttaatcg cagccggtgt cgacccatca gatattgttc tcgattacgc
      481 aggctttcgt acgctggatt ccatcgtacg tacacgcaaa gttttcgata ctaatgattt
      541 cattattatc acccaacgtt tccactgtga gcgggcatta tttattgcgc tgcatatggg
      601 gattcaggct cagtgttatg ccgtaccgtc accgaaagat atgctgtcag tacgtattcg
      661 cgaatttgcc gcccgtttcg gtgcgctggc tgacctttat atttttaaac gtgaaccgcg
      721 ttttttaggg ccgctggtcc ctattccggc tatgcaccag gtgccggaag atgcgcaggg
      781 gtatcccgcc gtcacacccg aacagttact tgaattacaa aagaaacaag gaaagtagtt
      841 atggatgtac agcagttttt tgtcgttgcc gtttttttcc ttatcccgat attttgtttc
      901 cgcgaagcat ggaaaggctg gcgcgcaggc gcgattgata aacgggttaa aaatgcaccg
      961 gaaccggtgt atgtctggcg agcgaaaaat cccggactct tcttcgctta tatggtggca
     1021 tatatcggct tcggaattct atctatcggc atgattgttt atcttatttt ctatcgttaa
     1081 tattttccct tttctgttaa ttattgagaa taattattac ttcactcaat aagctgcgga
     1141 tataacattc tgaaccgcag ctatttgtga atcctttcac agtttaaatt tccccacgcg
     1201 catagcctta atatcagcac gcaatattta tttagcgttc gccttaatta ctcatagcat
     1261 taaggaagat cacatgccgc aacaaaatta tctggatgaa ctcactccgg cttttacgcc
     1321 tttactggcg attaaagaag cctctcgctg tttattatgt catgacgctc cctgtagtca
     1381 ggcttgcccg gcacagaccg atccggggaa atttattcgc tcaatctact ttcgtaattt
     1441 taaaggcgct gccgagacaa ttcgcgaaaa taatgccctc ggtgccgttt gtgccagagt
     1501 gtgcccgacg gagaaattat gccaaagcgg ttgtacacgt gccggtgttg atgctcccat
     1561 tgatatcggt cgcttacagc gttttgttac tgattttgaa caacaaactg ggatggaaat
     1621 ttatctgccc ggtactaaaa cgctcggcaa agtcgccatt attggcgcag gtcctgccgg
     1681 attgcaggcc agtgtgacgc tgacaaacca gggttatgac gtcacgatct atgagaaaga
     1741 agcacaaccc ggtggctggt tgcgtaacgg cattccgcaa ttccggttac cgcaatcggt
     1801 gctggatgca gaaatcgccc gtatagaaaa aatgggcgtg accattaagt gcaataacga
     1861 aatcggtaaa acactcaccc tggagcagct gaaagcggaa aaccgcgcgg tactggtcac
     1921 cgtagggtta tcgagcggtt ccgggttatc gctgtttgag catagcgacg ttgagattgc
     1981 cgtcgacttc ctgcaacgtg cacgacaggc gcaaggcgat atcagcattc cacaaagcgc
     2041 attaattatc ggcggcggtg atgttgcgat ggacgtagcc agcacgctga aagttctcgg
     2101 ctgtcaggcg gtaacctgcg tagcgcgtga agagttagat gagtttccgg caagcgaaaa
     2161 agagtttacc agcgccagag agctgggcgt ttcgatcatc gatggattta caccagtagc
     2221 cgtggaaggc aataaagtca cctttaagca tgttcgttta ccgggcgaac tgacgatggc
     2281 ggcagataaa attattctcg ccgtcggtca gcatgccaga ctggatgcct ttgcggagtt
     2341 agagccgcag cgtaacacca tcgaaacgca acattaccag acccgcgacc cgcaagtctt
     2401 tgctgctggc gatattgttg agggtgacaa aaccgtggtc tatgcagtga aaaccgggaa
     2461 agaagccgcc ggggcgattc atcactattt agagggagct tgctcatgtt aacgaaagat
     2521 ctttcgatta ctttttgcgg cgtgaagttt cccaacccgt tctgcctttc ttcttcgccg
     2581 gtaggcaact gctatgagat gtgtgccaaa gcctacgaca caggttgggg cggcgtggtg
     2641 tttaaaacga tcggtttttt tatcgccaac gaagtctcgc cgcgttttga tcatctggtg
     2701 aaagaagata ccggttttat cggcttcaaa aatatggagc agattgctga acatccgttg
     2761 gaagagaatc tggccgccct gcgtcggctg aaagaagatt atccggacaa agtattgatc
     2821 gcttcaatca tgggcgaaaa tgagcagcaa tgggaagagc tggcgcgtct ggtgcaagaa
     2881 gctggcgcgg atatgatcga gtgtaacttc tcctgtccgc aaatgacttc tcatgcgatg
     2941 ggtagcgatg tcgggcaaag cccggagctg gtagaaaaat attgtcgggc agtaaaacgt
     3001 ggttccacgc tgccaatgct ggcgaagatg acgccgaata ttggtgatat gtgcgaagtg
     3061 gcgctggcgg cgaagcgcgg cggcgcagat ggcattgcgg cgattaacac cgttaaatcc
     3121 atcaccaata tcgatcttaa tcagaaaatt ggtatgccga tcgttaacgg taaatcgagt
     3181 atttccggat attccggtaa agcggtaaaa ccgatcgccc tacgctttat tcagcaaatg
     3241 cgcacccatc cagaactgca cgatttcccg atcagcggta tcggcggcat tgaaacctgg
     3301 gaagatgcgg cggagttttt attgctcggt gcagcgacgt tgcaggtgac caccggcatt
     3361 atgcagtacg ggtatcggat agtggaagat atggcaagcg ggttgtcgca ttatctcgcc
     3421 gatcaagggt ttgattcgtt gcaggagatg gtaggtctgg cgaatcacaa tattgtcccg
     3481 gcggaagatt tagaccgcag ttatattgtc tatccccata tcaatcttga taaatgtgtt
     3541 ggctgtggac gctgttatat ttcctgttac gacggcggtc accaggcgat ggaatggagc
     3601 gagaaaaccc gcacaccgca ttgtaatacc gagaaatgcg tgggttgtct gctttgtggt
     3661 catgtctgcc cggtaggttg tattgatctt ggggaagtga agtttaagaa aggcgagaaa
     3721 gaacacccgg taacgttgta aaaccagcga cgcatccggc atttagttcc tgtcgccgga
     3781 tgcggcgtga acgccttacc cggcatacaa aacaatataa atcagtatgt tgtgctcatt
     3841 gtaggccgga taaaacgcgc cagcgttgca tccggcattt ggttcctgtc gccggatgcg
     3901 gcgtaaacgc cttatccggc atacaaaacc atacaactca tgatgttatg tgcatttctg
     3961 accggatgcg aaccacattc acatcacttc ttacgcgcgt atttcagtga atccagcgcc
     4021 acggcgaaga taataatcgc ccctttgatg atgtactgcc agtacgggtt tacgccgatg
     4081 tacgtcaggc catagttgat gacggtaaaa ataatcaccc cggtcaccac accaattacc
     4141 gtccccacac cgccgctgaa cgatacgccg cctaccacgc acgccgcgat agcatccagc
     4201 tcatacataa agccgaggtt gttggtggct gagccgatac gtccggcttc taacatcccg
     4261 ccgaaggcat agaacacacc agacaacgcg taaatcatca gcaggttcag gccgacgttg
     4321 acaccagata ctttcgccgc ttccgggtta ccgccaatgg caaaaatgtt cttaccgaag
     4381 cgggttttgt tccataacac ccagacaaac accaccgcaa tcaacgcata gaaggtgatg
     4441 taagagagac ggaaactccc cagcgcaata aagccttggg caaaagtgga gaaaccactg
     4501 tcaaaaccgg aaattggcga cgccccgaca aagtcgtaat agagcgagtt aatgccgtag
     4561 acgataatca tcgtaccgag cgtggtaatg aacggcgtca cgttgagata ggcgataatc
     4621 aagccgttga tcaaaccgat caccgcacca atggcacaga caatcagaat aaccagcgca
     4681 atcggcatcg tcgccatttc cgggaacact ttgttggcgt tatccatgga ctgcaataac
     4741 gtcgctgcta ctactgccgc cagccccacc tggcgacctg cggaaaggtc agtcccctgg
     4801 gtgacaatta accctgccac accaagcgcg ataataatac gcaccgatga ctgggtgaga
     4861 atattactta agttcaacag acttaaaaat gttgggtcct ggaaaataat aatcgccagc
     4921 aaaactaaaa gaacgacgta aataccgccc tctttcaggt aagtaagaaa acttttctta
     4981 tttaatgcac tcatgggaag cccctaatct taaaggtgca aagacgcaag acgcagaatt
     5041 tcgctttgcg ttgttgtttt tgtatcgaca attccggaaa cgagaccatt gctcatgacc
     5101 agaatacggt ctgttatccc taacaactct ggcatttcgg aggagataat aataatcccc
     5161 ttgcctttct tcgccagttc ggcaattaac tgataaatct caaacttagc cccaacatca
     5221 ataccgcgcg tcggttcatc gagcattaat atttctggtt gcgttaacag ccagcgacca
     5281 ataatcactt tttgctgatt accaccggag agcgaaccaa tttgcgtccg atgccccggt
     5341 gtttttaccc gcatcgaatc aatcacccac tgggtatcgc ttttcatccg cgagttatcc
     5401 agtaaaccaa ctttattttt atagttgcgg atattggaaa ttaacgagtt aaagccaata
     5461 tccagatagg cataaattcc cgttgagcgg cgctcttcag ttaccagtgc aaatccgtgg
     5521 tttatggctt cgttggcatt atggttattg atctttttgc cgtgcaacgt aatggtgcca
     5581 gccgatttct cgcgaatacc aaataacgtc tcaacaatat cggtacgttt cgctcccacc
     5641 agaccggcta taccgagaat ctccccttta tgcagatcaa acgagacatc gcgaatcgac
     5701 ggctggcgca gcgacgtcag gttacgcact tccagaatca cttcgcccgg cttgttttct
     5761 ttgtcaggga aacgctggtt aagagaacgc ccaaccatca tggcgatgat cttgtccatc
     5821 gtcagtcctg ccagcggttc ggtagcgatc cactgaccgt cgcgcaatac ggtaacttca
     5881 tcacataact ggaagatttc ttccatttta tgcgagatat aaacaatacc gcagccgcgc
     5941 tcttttaatt tacgaataat agtgaacaga tgattgacct ctttttcggt taacgaagaa
     6001 gtcggttcat ccataatcac aattttcgcg ttataggaaa acgctttggc gatttcgatc
     6061 atctgcattt gcgaaacaga taatgtaccg acgcgcgcac gcggatcgat atcaatatcc
     6121 agttcatcaa aaatcgcttt ggtttcgcgg tacattttgt cctgatcgac aaacatgcct
     6181 ttggtgggat atcgccccag ccacatgttg tccatcaccg aacgttgtaa taccaggttt
     6241 aactcctgat gtaccatcga aataccattt tccagtgctt ctttggcgga atggaaatcg
     6301 atctctttac cctggaataa aatggtgccg gagtcttttt gataaatacc aaacaggcat
     6361 tttaataatg ttgattttcc cgcaccattt tcccccatta atgcatggat agaatgtggt
     6421 cggactttta agttaacgtt atcaagtgcc ttaacaccag gaaaagactt gttgataccg
     6481 ctcatttcca acaagtattc cccggaagac ggagtcgttg agctgaccat ataattttac
     6541 cttgttggtc atacaataag ggcgcagtaa acgactgcgc ccaatcagtc ttatttcttg
     6601 ctgaattcag ccaggttgtc tttatctacg ccaacataag gtacgcggac cactttgttg
     6661 tcgattttcc agttggtgcc atcagccgca cctttgccat cggccaggtt tttcgccaga
     6721 tcaaaggtcg ctttcgcctg gttgttagca tcgttcagta cggtgcccgc cagtgcaccg
     6781 gatttcacca gcgccagcgc ttctggcagc gcatcgacgc caaacaccgg aatgctggac
     6841 ttattgtgtg ctttcagtgc ttctaccgcg cccattgcca tcgcgtcgtt gttggcgata
     6901 accacctcga ttttgttggc gttcgggcca gacagccagg cgtccatctt atctttcgcc
     6961 tgagcggtat cccacatagc ggtatctaac tgtaactgtt cagttttaat gcctttgtcg
     7021 ttcaattctt taatcacgta agtggtacgt gcttctgcat ccggatgccc cggctcacct
     7081 ttcagcagta cgaattgaat ctgaccgtct ttgttcagat cccaaccctg attcgccgcc
     7141 cagtgtttag caatcaaatc gccctgaata atgccggact ctttggagtc agtgccaacg
     7201 tagtaggctt tgtcgtagct atccagcgcc ttacgagacg gttctttgtt gaagaaaacc
     7261 accggcacgt tttgcccacg cgctttctca atcaccgtac ccgcagctgc cgggtcaacc
     7321 aggttgattg ccagtgcctt cacccctttc gccagcaata cgtcgatctg atcgttctgc
     7381 ttggactggt cattctgaga atcattcatc agcagctgaa catctggcgc ggctttcgca
     7441 tcttgctcaa tagccttgcg cactacagac ataaagttat cgtcgtactt atagattgtt
     7501 acaccaatgc gagtatcagc agcgtgtgca gcggcaccga ataacatgct ggccatcaca
     7561 gcagacaggg ttaacacctt cttattcatg gtatctccgg tttttcttat gcagggtagt
     7621 gcttgagata aatgctcggc ggggcagtag agttaatgaa gtgttactga acgccgaagc
     7681 tcacttttta aaattcgttc ttccatgccc ggtaacgctc cagaaaacgg ctttaattgt
     7741 tgttatgacg ctattacctc ggtgaaagtg attattcact gttacatacg ggttacaacg
     7801 ttaaaacggt gcaatcatag ccatcacatt gttaagatac tgtgaaatca ctcacagatt
     7861 gaaagcggtt acatcgcctg atttgttgag ttagtgatcg ccgccgcatt ctggcgtgtt
     7921 gcgacagaat ggcgacgcac taacgtcggc ataaaacagt ggctggcacg aggatcaata
     7981 ttgtctgctg ccccctgcaa ggccagttcg gtagctaatt tagccattga agcaatggga
     8041 taacgcacag tcgttaattg cgggtcggtg taacgggcga tgggaatatc atcgaaacca
     8101 atgattgaga gatgtaacgg aatcgcaatg ccattatctt ttaatgctgt cagtgcgcca
     8161 gcggccatat tgtcgttata agcaaataca gcggttagtt gtagattgcg ccccagcagt
     8221 tcaaccatcg ccgcctcacc gcccggcatg tccggcgtac cagtgccaat ccagctttcc
     8281 ggcggaataa tatcctgctc tttcaacgca ctcatccagc ctgctttacg catggcgtca
     8341 tcttcaatgc cgtggctgga agaaagataa ccaatacgtt gatgaccgtt attcagcaac
     8401 atgcgcgtcg ccattcgggc accgctgaga ttatccaggc aaacgcaacg atgggcgtac
     8461 cccggcacaa cgcggttgat taacaccata ccgggaatgt tatccataaa ttgtgccagt
     8521 tcatcgtcac tcaatgcttt tgagtgaaca atcaacgcat tacaacgctg gtgaattaac
     8581 acctcaatgg cgtgacgctc tttttccgct tcatgatagc tattgccgat tagcacgtat
     8641 ttctgatgct gctgggcgac cagatccacc gcttttacca gcgcgccgaa aaaagcatca
     8701 gaaacgtcca tcaccaccac gccaatggtg tcgctaacct gagttgccag cgcctgggca
     8761 ttggcgtttg gccgataatc cagctcactc acagctttca ttactgcttc acgcgtgtcg
     8821 gcactgacca gcgtgctgtt attgagcacc cgggaaaccg ttgccacaga gacgccagcc
     8881 tgacgcgcta catcacgaat ggtgatcata ttcactacct gttatgagat tgcagtaaat
     8941 gactgcttgc tggcggctat tttgtcagca ctgaatacag gacttcgtga atcgagtcac
     9001 agcaatggaa acggttacag ccgttttgtt aacgaatgtg atccagatcg ttatcttgat
     9061 gttttagata ttgtcggtcc ggcagcacgc aaagttaact gacgccagag ccattccacc
     9121 ggcccctggc ggaagtaacg cagccagata acagagaaga agatattcgc cagccatacc
     9181 ggaataacaa acgccagcag ctccagacgg tcaaactgca taaacaaacc gaggtggtaa
     9241 aaaagcgtag tacaaatcag cgtttgcaat agatagttgg tcagtgccat ccgtccgaca
     9301 caggcgattg ccagcaccag cttaaagcgg cttaattgcg gccagaagcc ataaaacagc
     9361 gacgcatagc caatcgcctg aaacggcgca ctcagttccc gcggcatctg aagtaagaag
     9421 gcgcaccaac gatacgccca gtccagtcgc cattgcaggg cgatggcagg aaggttaatg
     9481 atcaccccaa tcgccaccag cacaaaacca gtacgacgat agtgacgtaa gctgaactgc
     9541 cctttcagcc agccactgcg catcaatgcg gcaccaatga gcatcatccc tgccagttgc
     9601 cagccatact gtgcgcccag tgccagtaaa ctgttgccaa caccatcggc acgattactg
     9661 atcgcttcaa caccaccgtg aagtttccag tgcttttcat acaaaatagc cgatgtatca
     9721 ggtgtccagg cgcggctggt ctggctatcg gaaatccccc ccaataacag caaaacgcca
     9781 agccccacca gataaagcat gacgccggta ttaaacaggc tttttaccga tggcgcatcg
     9841 cgcaccagcc gccagcagat taagcccacc agcccgtaag ccagcagaat atcgccgtcc
     9901 cagaacaata aaccgtgaat aaagcccagc aagactaaca gcgttaaccg cgactggatc
     9961 catcgtctgc cacggggcag caacatttgc aggcccgcac caaacaacag cgcaaaaagc
    10021 gtgaggaatt tcacctggcc gatgagatcg agaaatgccc aggtccatgc atcctgtggc
    10081 gtaatagcgc cgtaccaggc gggattgaga taagccgcct ttggtaaccc aaaggcgctg
    10141 atgtttaata gcaggatccc cagaatggcg acgccgcgaa caaaatcgag cgtgacgttg
    10201 cgctccacgg ttcctgcctt tttatcagtt gtgatgacgc acagcgcgca gaaactcgtg
    10261 gcgcgtattc tgactggatt tgaacaatcc accaagagag gtcgttgtcg tggcactggt
    10321 tgcatcgcgg atgccacgcg ccttcacgca gtaatgcacc gcgtcgatag agacagccac
    10381 gttattggtg cccagcagcg tttgtagcgc aatgagaatt tgctgcgtca gacgttcttg
    10441 tacctgcgga cgctgggcaa agaactgcac aatacggtta atttttgaca gaccgatgac
    10501 cgaatctttc gggatatagg ccaccgtcgc tttgccatcg atggtaacaa aatggtgttc
    10561 acaggtgctg gtcagagtga tatcgcgcac ggtgaccatt tcatcgacct tcattttgtt
    10621 ttcaatgagg gtgattttcg ggaaattggc gtaatccaga ccggagaaaa tttcatcgac
    10681 atacatttta gcgatgcgat gcggcgtttc catcaaactg tcatcagcca ggtcgagatt
    10741 cagcagctgc atgatttcgg tcatatgacc agcaataagg cttttgcgcg tttcgttatc
    10801 catttcatgc acgggcgggc gcagcggtgt ttccagtcct cgcgcaacta acgcttcatg
    10861 aaccagggcc gcttctttac tgagtgatgg catttatgat ttctcctgca ggtgtgacgc
    10921 ctccgccctg cgtgggggca aagttattaa gctgatttac agcctgatta ttgtgcgtga
    10981 ggcggcgcac ataatccagt attcacagcg ataattattg taattgccgc tgcctttcat
    11041 cagcagatgt taaaacatcg ttatgcaaat acggaagtga aagttactca cagcacattg
    11101 aataaacggt atgatgaaga aattgcaaac aacacaacaa ggagccacgc atggaaatgc
    11161 tcgaagagca ccgctgtttt gaaggctggc agcaacgctg gcgacacgac tccagtacct
    11221 taaactgccc gatgacgttc agtatctttc tcccaccacc tcgtgatcac accccgccac
    11281 cagtgctgta ctggctttcc ggattaacct gcaatgacga gaacttcacc accaaggcgg
    11341 gtgcccagcg cgtggcggcg gaactgggga ttgtactggt gatgccagac accagcccgc
    11401 gcggcgaaca ggttgccaac gacgatggct acgatttagg ccagggcgcg gggttttatc
    11461 ttaacgccac gcaaccaccg tgggcgacgc attaccggat gtatgattat ctgcgcgatg
    11521 aattaccggc gctggttcag tcgcaattta atgtcagcga ccgctgcgct attagcggtc
    11581 attccatggg tggtcacggt gcgctgatta tggcgctgaa aaatccgggt aaatacacca
    11641 gcgtttccgc ctttgcgcca attgtgaatc cgtgcagcgt cccgtgggga atcaaagcgt
    11701 ttagcactta tttaggtgag gacaaaaatg catggctgga atgggacagt tgcgcgttga
    11761 tgtatgccag taacgcgcag gatgcaatcc cgacgcttat tgatcagggt gataatgatc
    11821 agtttcttgc tgaccaactg caacctgcag tactggcaga agccgcgcgc cagaaagcgt
    11881 ggccgatgac gctgcgtatt cagccgggat atgatcacag ttactacttc atcgcctctt
    11941 ttatagagga tcacctgcgc ttccatgcgc agtatttact gaagtgaaag tccgcccggt
    12001 tcgccgggca tcttctcatc agaagcgata atccactgcc ataaagtaac gacgtccgtc
    12061 ttcgttatag ctgtagtcgt cacgactgag atccttgtcg ccaaggttca gcacgcctgc
    12121 gcgcagtttg acgtctttag tcacctgcca ggccgcgccg gtattccaga tggtataacc
    12181 gcccggtgtt ttagccgtcg cgctgtcggc gcgtttctgc ccggtatagt gaccagaaac
    12241 atagaatgac cagtcttcca gcgccagcgg tttccagtcc agcgtaccgt tagcagtatg
    12301 gaacggcaga tcggatagcg gtttgttttc gccgttgctg acatcacgac catcgttgta
    12361 ggtgtagttg atcgacagtt tccattcatc gttgaacgga attttcagtt cggtttccac
    12421 gccctgaata cgagctttgt taacgttgta gtagctaaat accggtatgc gccgtccgtt
    12481 agcgcccgtc tcaaaaccga caaagttttg gtagcccggt gcagcattga cgtcagacgt
    12541 acggctaatg ctgatacgat ctttcacatc gttacggaaa acggtaacgc tggattcaac
    12601 cccttccagc cagccttctt cacccatgta gtaaagcccc agctcccaac tttcgctggt
    12661 ttctggtttc agatccgggc tacccacaat cttacatgcg ccacggcagg aattgctcgt
    12721 ccagtcaggg ctaagttgca acagagaagg ggctttaaat gccgtcgccc agcccccttt
    12781 caccgttacg gtgtcggtgg cgttataaac caggtaggca cgcggactcc agtgttcacc
    12841 gtaggtttcg tgatcgtcca tacgcacgcc ggtcgtcagc gccagcggct cgaagatccg
    12901 ccattcatct tccacaaaca gcgcgtactg gctggcagac gttttggagc tgcttccccc
    12961 ggtcaggttc accgcatcgc taagtttgtc gtgacgccat tcaccgccaa ccgtgagaaa
    13021 ctgattaatc gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc tttcggaagt
    13081 tatcgggctg ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt atttcagttc
    13141 gctggtgccg taatcccaac gcccattatg gctgacggag tagttctggc gttccaggcg
    13201 gtttttgtcc agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc cggcagtaaa
    13261 atcgtgattt tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc tggagaatcc
    13321 ttcaatacgc ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt catccttttc
    13381 acgttttgcc aggctgccgt aagctttcat tcccagcacg ccgtcaatta atggtccgct
    13441 ggtaaagaat tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa cggtggtatc
    13501 gacggtaacg gtgcccgacc atttctgacc gatttttttg gtgatgatat tcactacacc
    13561 gccgagcgca tcggaaccgt acagcgacga catcgggcca cgaaccactt caatacgttc
    13621 gatggaatcg accgggatcc agttcagatc gaaatcattg tggcggaaga cggcattgcg
    13681 ggagttaacg cgtttaccgt cgacaagaat cagggtgtag ctgctgtcca gaccacgaat
    13741 acttacgccc ttacggttat ccccttcgtt cgtcagttgt acgccaggca cttctttgag
    13801 gacatccttc agattctgta ccggttttcg ctgcaggtct tcctgggtaa tgacgctgat
    13861 actggcggga gcatctttaa ggttttgttc aacggaagat gcagtgacaa ccatcgtttc
    13921 gccatcatca tcgaccgcta acacaggcca tgcacaagaa atagcggaca aacacagccc
    13981 gacccgtacg aaagggttca acctaaacat tccatatctc catgaggtaa ctacgaaaat
    14041 aaaatgggta tcgctcacat cttctttacg tccccttgcg tacggcagca tcgcggtgga
    14101 cttatttgta tgcaggtgat catcccagaa agccggaaca gcctctgatt tgtcagcctt
    14161 ttttgattgc ggcgtaacga taatgcaaac gataacaatt atcaatccaa atgttaaatt
    14221 ttatatccgc atgatttgtc agggaaatgg aattgaaaaa gccctctcgg ttgagagggc
    14281 ttagcaagga agggaggaaa cttatttctt atcgttctgc gggaacttca tttcgctgta
    14341 gcgtacgaag tgagttcctt taatcagctt atagccgaac caaataatca ggaacagcgg
    14401 gataccaata tacgttgccg ctacgccgcc ccagtcaatg gtatctttca ggaacgcttc
    14461 gtagttctgg cccaaagtga taatcagaca cagaatgaat gcgaagatcg gccccattgg
    14521 gaagaaacct gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata cgtacccgcg
    14581 acggaaacga tagtggctaa tggcaatccc cagccaggcg ataaagcccg tcatcccgga
    14641 ggtgttcagc agccacaggt ataccgtctg gttgccaaac atggaggtca ggaagcacaa
    14701 accggcaatc accgtcgtcg catacagcgc attacgcggc acgccaccac gcgacagttt
    14761 agcgaaaatg cgcggcgctt taccgtcaca cgccagggta tacagcatac gagtagacgc
    14821 atacataccg gagttacctg ctgacagcac cgccgtcaga ataactgcgt tcatcaccgc
    14881 cgccgcagag agcagacccg cgtgctggaa caccagggtg aacggactga cgctgatgtc
    14941 tttaacatcg ttacgcagca ggctcggatc ggtatacgga ataatcaggc tgataatcag
    15001 gatcgcgaac acatagaaca acaggattcg ccagaatacc tgacgtaccg cgcgtggaat
    15061 gtttttcgcc ggatcttcgg actcgcctgc agcaataccg atcagctcgg ttccctggaa
    15121 agagaagccg acaatcatag ctacgccgat catcgccgca aaaccaccag caaacggcgc
    15181 ttcgccgatt gtccagttgc tccagcccgc aggctgcgcg cctttgaaga taccgataat
    15241 catcagcacg ccaacgatga taaagacaat aactgtcgtg actttgatca gtgagaacca
    15301 gtattccgct tcaccaaagc cgcgaacaga gatgtagttc aacaggaaga taacgccgag
    15361 gaacaacgca ctccagatcc agcccggtgt atccgggaac caccagctca tgaccagctg
    15421 tgctgcaacc aggtcaacgg caatagtcac cgcccagttg taccagtagt tccagcccag
    15481 cgcgaagcca aagccttctt caacatagtt ctgaccgtaa gtggcaaacg aaccggaaac
    15541 cggcatatat gcagccagtt caccgagact ggtcatcagg aagtaaacca tcaggccaat
    15601 cagcatatac gagagcaatg ccccgcccgg acctgcctga gaaatcgttg cgccagaggc
    15661 aacaaaaaga cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt gacgcgcctt
    15721 caattcacgg cgtaagcccg gcgcttctgt agttttagtt tcggaaccca tacaaaaatg
    15781 ctatccatct taaaaatgag gcgcgattgt agcagacgat tggcaatcct tccggcagaa
    15841 atacacggtt ataagacacc ttcatgatcg cccagggatt ataagtaaag cagccaatct
    15901 cttttctggc gaatgcatgc tgagggcgct gcgtcacgaa atcgacacag caccagcatg
    15961 ttcttgtaca gcaccttaac gcggtacatt cgcgggatcg caatagtcca gaaagcgccg
    16021 tagcgcgttg gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac gcatcaggcg
    16081 cggcagaggg accgcaactt cacttaatgt gcctgcctgc aattgatctt caatcacccg
    16141 tcgcgacagg caactaattc ccaacccatg acgcaccgca tgtttgattg cctcggagtt
    16201 acctaattcc atcgccatct caaacttcgg taaatgtgac agcaacagat aatcgacaat
    16261 ctcccgcgtg ccggaaccgc gttcacgcag gatccacggc gcagcggcca gctgttctaa
    16321 ggtgaccgga ccacgagcca acggcgaagt cggcgcggcg aaaaccacca gctcgtcttc
    16381 cagccacggt tcagaaatga tttcagtgct gtggcacggt ccttcaataa agccaatatc
    16441 aacgcggaaa tccagcaccg cttgcatcac gtcctggcta ttcccaacgc taagttcaat
    16501 cggcaactgc ggataatcat ggcgataacg ggcgataact gcaggcagaa tgtagttacc
    16561 gatggtacta ctggcataga tacgaatcgc gccgttgtct tcgcgaaaca gttgttcgat
    16621 ttcaaccgcc tgttcaagca gtgccaacgc tcgcggatag agcagccgcc cgtgttcatt
    16681 aacaaccagt cttttcccca cgcgatcaaa cagttgaacg ccaagctgcc cttccaggtc
    16741 ggtcaaggct gcgctcactg ctgattgcga caacgccagc atcaccgacg cctgggtggt
    16801 tgatccactt ttcaatactt ctgcaaaaac ttccaactgc cggagggtga tgtgcatagt
    16861 cgcttaccac ttataaagat taattataaa tatataatca attttatttt taaaccagtt
    16921 agtcgtaacc ttatgcccgg ttaaaggaga gggttatgac gaatatcacc ttacagaaac
    16981 aacatcgtac actgtggcat tttattccgg ggttagccct gagtgcagtt atcaccgggg
    17041 tcgccctgtg gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt gccctcaccc
    17101 tcgcaatctt gttggggatg gttttaggca acaccattta cccgcacatc tggaaaagct
    17161 gtgacggtgg tgtgctgttt gccaagcaat atttattacg tctgggtatc attctttatg
    17221 gcttccgtct gacgttctcg caaattgccg atgtcggtat cagtgggatc atcattgacg
    17281 tgttgacgct gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa gtgtttggtc
    17341 tggataagca caccagctgg ttgatcggtg ccggtagcag tatctgtggt gctgccgcgg
    17401 tactggcgac tgagccggta gtgaaagcag aagccagtaa agtaaccgtg gctgttgcaa
    17461 ccgttgttat cttcgggacc gtcgcgattt tcctctaccc ggcgatatat ccgctgatgt
    17521 ctcaatggtt tagtccggaa actttcggta tctacattgg ttctactgtg cacgaagtgg
    17581 cgcaggtggt ggcggcaggt catgccatca gtccggatgc agaaaacgca gcagttattt
    17641 ccaaaatgct gcgcgtgatg atgctggctc cgttcctcat cctgctggcg gcgcgtgtta
    17701 aacagctgtc tggggcgaac agcggcgaga aaagcaaaat cactattccg tggtttgcca
    17761 tcttgttcat cgtcgttgcc atctttaact cgttccacct gttaccgcag agcgtggtga
    17821 acatgctggt aacgctggat accttcctgc tggcaatggc gatggcggcg ctgggtctga
    17881 ccactcacgt cagcgcgctg aaaaaagccg gggcgaaacc tctgctgatg gcactggtac
    17941 tgtttgcctg gctgattgtt ggtggtggtg cgattaacta tgtgattcaa agcgtcatcg
    18001 cataaaccac tacatcttgc tcctgttaac ccgctatcat taccgttttc ctccagcggg
    18061 tttaacagga gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc tggcggtctg
    18121 gcaaatgccg caattcgcgc cgccgaaatc gacgcaaccg cgtttgcctt gttcaccaaa
    18181 aaccaacgtc agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga attcaaagcc
    18241 gcctgtgaaa aatatcacta cacatcggcg caaattcttc cccacgacag ttatctgatt
    18301 aacctcggac atccggttgc tgaagctctg gaaaaatcgc gcgatgcctt tatagatgaa
    18361 atgcagcgtt gcgaacagct ggggctttct ttgctcaact tccaccctgg cagccatctg
    18421 atgcagattt cagaagagga ttgccttgcg cgtattgccg aatccatcaa cattgtgctg
    18481 gataaaactc aaggtgtcac cgcggtgatt gaaaacaccg ccggtcaggg cagtaactta
    18541 gggtttaaat tcgaacatct cgcagcgatt atcgacggcg tggaagataa atcccgcgtc
    18601 ggcgtctgca ttgatacctg ccatgctttc gctgccgggt atgatttgcg tactcctgcc
    18661 gaatgcgaga aaacgttcgc tgattttgcc cgtattgttg gctttaagta tctgcgcggg
    18721 atgcacctca acgatgcgaa aagcaccttt ggcagccgcg ttgaccgtca tcatagcctc
    18781 ggcgaaggca atatcggtca tgatgcgttc cgctggatca tgcaagacga ccgtttcgac
    18841 ggcattccgc tgatccttga aaccatcaac ccggatatct gggcagaaga gatcgcctgg
    18901 ctgaaagcgc aacaaactga aaaagcggtg gcctgatcga tgaacgacag ggaaaagcag
    18961 attcttaaaa ttttacggcg taacccgctg attcaacaga atgaaatcgc cgatattctg
    19021 caaatcagcc gctcacgtgt tgccgctcat attatggatt tgatgcgcaa gggtttgatt
    19081 aaaggcaaag gatatatcct taccgagcag gattactgtg tggttgtggg tgcaatcaat
    19141 atggatattc gcggaatggc ggatatccgt tacccgcaag cagcctctca tccaggttct
    19201 gttcactgtt ccgcaggcgg cgtcggacgt aatatcgcgc ataacctggc tctgcttggt
    19261 cgggatgtgc atttaatttc agctattggc aacgattttt acggcgagac attactggaa
    19321 gaaacgcgcc gcgcgggcgt gaacgtttca aactgcattc gtctgcatgg acacagcact
    19381 gcaacttatc tcgctatcgc taataagcag gaagaaacta tcctggcaat caatgacaca
    19441 catattctgc aacagctaac gcctcaactg ctcaatactt cacgggactt gattcgccat
    19501 gctggtgtgg tgctggctga ttgtaatctc acacctgaag ccctggagtg ggtctttacc
    19561 attgctgatg aaattccgat gtttgtcgat accgtttccg aatttaaagc gaacaaagta
    19621 aaaaactggt acagccggat ccacactctg aaaccgacac aaaatgaact ggaaattttg
    19681 tgggggcaac cgattaaaga cgacaacgat cgcatcagag ctgtaaattc gctgcatcaa
    19741 caaggtgtta aacgcatttt tgtttatctg aaagatgaat ctgtattttg cagcgataag
    19801 gatggagaac aatttttgct gaccgcgcca gcgcatacga cagtagacag ttttggtgct
    19861 gacgatggtt ttatggcggg cctggtatat agctttcttg aaggatacag tttccgcgac
    19921 agcgcccgtt ttgcgatagc ctgcgcggca atttcgcgcg ccagcggcag cttaaacaac
    19981 cctaccctgt ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg
    20041 attcgacgct tgaccgcatc ttatccggct cataaattgc gcactacgct aaaccaataa
    20101 agaatcctgc aatagtagca ctcatcaggt tactgacagc aaaaacgcaa tagccagtaa
    20161 taccaccatt cccagaacac ttctcatgac atccatagtt attatctctt catgtcattt
    20221 tgggcgacgg ctctctgccc atcataaata gccgggcctg catcttgcga tactgacccg
    20281 gcttgttatc aacacttaat tgggttttat gtagccgcgt acgcactctt cgactaatcc
    20341 ccagaaccag tcggtatcaa tagtgatgcc gactttggta ttggcgggct tgccaagaac
    20401 acccagctcg tcgcagacgg tacgcccata gcaagggcca ctgttaacgt ccacttcgac
    20461 gtacatctcc tgggttttaa tgccatcagg gttaatcaaa taaccgatgc aggtggcgtc
    20521 gtgcaccggg ccgccagcca ggccgtagtt ttcgaactgc gttttgagag tgaagttcat
    20581 gatgtcgctg aacagctctc cggcagggcc gccagccctt tccatccgag caatcacatc
    20641 cggggtgcaa acggtctggt tggtaagatc gaggcccatc atcactaatg gaacgccgga
    20701 ggtgaacacc acgcgtgcgg cctccgggtc agcaaagatg ttgaactcag cagatggggt
    20761 gaagttgcca gtaccataag caccgcccat cagaacaatt tcacggattt tcggcaggat
    20821 cgcgggttgc atacgcattg ccaccgcgat gtttgacaac ggaccaaccg gcaccagagt
    20881 gatatcgcca tcgcttgcca taagggtatc gatgatatat ttcaccgcat gagtgttttc
    20941 tgcctggcgg gtcagcggct cgaataccgg gccatccagt ccggtttcac cgtgaatatt
    21001 atcggcaaca atttgttgac gcataatggg ctgcggcatc cccgcataaa ccggaacatt
    21061 aatctccagt ttctggcaaa cattcaggcc attaattaat gttttatcga gggtttgatt
    21121 acccgctaca atggtgatac ctaataaatc aatcgccgga tgtttcgccg ccatcattat
    21181 agcaatagca tcatcatgac ccggatcaca atccagaata atttttctct tttccattgt
    21241 ttatttcctc tgtttccagt tgcgttattg tttctacagc aaagaaaatt cgacctggcc
    21301 gatgattgaa tcttaacaac agcgtacgta tgctaaatat gagaaatctc atagcggata
    21361 aacatcgtga aagaaatcca caataatgat cttaagcagc aattgataag tgaatctgcg
    21421 tttaaggatt gcttttcaac ggatgtttca gccgatacgc ggctgtttca ttttttagcg
    21481 cgtgactaca ttgtgcagga agggcaacag ccgtcctggc tgttttacct gacgcgaggc
    21541 cgcgccaggc tttacgtcac gcttgctaat ggtcgagtgt cgctgatcga tttctttgcc
    21601 gcgccctgtt ttattggcga gattgagtta atcgacaaag accatgaacc gcgtgcagtg
    21661 caggctattg aagagtgttg gtgtcttgcg ctccctatga agcattaccg cccgttgtta
    21721 ttaaacgaca cgctattttt acgaaaactc tgcgtcacct taagtcataa aaattatcgt
    21781 aatattgttt ctttaactca gaatcaatca tttccgttag tgaatcgcct ggcagctttt
    21841 atattactct cgcaggaagg tgatctttat cacgaaaagc atacgcaagc ggcagagtat
    21901 ttaggcgttt cttatcgaca tcttttatat gttcttgcgc aattcattaa tgacggttta
    21961 ttaattaaaa gcaagaaagg gtatctcatt aaaaacagaa agcagttgtc aggactggcg
    22021 ctggagatgg acccggagaa taaattctcc gggatgatgc agtaaaaatt attggcaata
    22081 gtgcgattgc cggatgcaac gcttaataag gcgtacatcc ggcaatgaaa ctcaagctaa
    22141 accaataaag aacccggcaa tggtcgcgct catcaggttg gaaagcgtcg ccgccgtcag
    22201 cgcccgtaaa ccaagctggg cgatttccgg cgcgcgtttt ggcgaaatag ccgaaaacgc
    22261 tccaacgaca acaccgatag aaccaaagtt agcaaaacca cacagcgcaa aggagatgat
    22321 cgccacggtt ttcacttcca gcgtgccgcc cgtttgcaga tatggcgaaa aactcaggta
    22381 agcgacaaat tcgttaatcg ccagtttctg cccaatcagg ctacccgcaa ggttggcatc
    22441 actccagtcc acacccatga tccacgccaa cggggccagc acatagccaa aaatactttc
    22501 cagagaggca ttggcaaaac caaaccagcc gccaattccg ccgataatac cgttgatcag
    22561 cgcaatgatc gccacaaatg ccattaccac cgtcgccaca ccggcggcga ttttcagccc
    22621 ggtcattgca ccgttcgccg cagcttcaat aatgcttttt ggcggtgttt cgctaaacga
    22681 cagattttca aacgtaactt gcgaaggttc cgtcgccgga ctaagaatac gtgcaaacag
    22741 gataccgcca ggaatagcca tcagcgaagc ggctaacaaa tagtcaatcg gcacgcccat
    22801 tccggcatag ccaatcatca tcgaacccgc aatagacgcc atgccgctac aaatagccgt
    22861 aaatagctcg ttgcgattca tgcgatcaat aaaaggtttc acgatcgccg ggatctcatt
    22921 ttgcccgagg aaaatagtgg tgactgcgac aaaagattcg attttgctga tgttgagggc
    22981 tttctgaaaa atactgccaa gaatacgaat cagcagcccc attacgccaa tgtagtacag
    23041 caagctgata agcgcggtaa cgaaaataat cgccgggagt acgcgaaagg cgaagataaa
    23101 gcccgcaccg tcaaacagaa catccatttt gggaccgacc agcgaaccaa aaataaaggc
    23161 acttcctgcg tcactgtagg acatcacttt atggacgcct aatgccgcct gctctactgc
    23221 ccattttccc gggggaaaat agagcatgat gccaccaatg gcaatttgca gcagcagcgc
    23281 agccccaacc gtgcgcaagc tgatgctctt tttatttacc gacaacagaa atgctattgc
    23341 cagtaacacc accatcccca caacacttct cattatatcc ataatgattt tcccttcatg
    23401 ccagtaaacc cggcgtcagc gccaggtttt ggtatgtttg atgagtacgg gcgacggctt
    23461 tctgcccacc agaaattaac ccacgagacg ctgatattct ttggcaattt cgctcgccag
    23521 aatggcgtta ttaaagacca gttgaatatt ggatttcagg ctatcaccgc cggttagctc
    23581 cgcaacacgc gccagcagga acggtgtact ctctttccca ataacaccct gttcttcggc
    23641 ttcagcaacg gcttgatcaa ttgctgcatt gatggtttct tctggcatcg caaactgttc
    23701 cgggatcggg ttcgctacca ccaggccgcc gttcaaaccg gtttgccact tcactgccat
    23761 cgcgcgagca atttcgcggg cactgtcgag acgaatgctg acatcaaatg ggctggttcg
    23821 gcagaaaaac gcaggcagcg cttttgtctg ataaccaatt aacggtacac cgaaggtttc
    23881 taaatattct gttgttaatc ctaaatcgag aatagatttt gcccccgcac aaacaacagt
    23941 gacattggta tttgccagtt cctgtaaatc ggcagaaata tcgaaggtat gttccgctcc
    24001 gcgatgcaca ccaccaatac ctccggttgc aaaaacttta atcccagcca gtgctgcaat
    24061 aatcatggtt gatgcaacgg tagtcgcgcc atttttccct gcagcaacga caaaaggtaa
    24121 atcacgacga ctaactttcg tcacgttatg tccttcacgg cccagcaatt cgatttcttc
    24181 tttacttaag cccactttca tcacaccgcc aataatggcg atagtggctg gtactgcgcc
    24241 ctgtttacga attgtttctt ctacttcaat tgcggtttgg gcattttgtg ggaatggcat
    24301 tccgtgagaa attatggtcg attccagcgc aacaaccggt tttttatttt ttaaagcatc
    24361 ctgtacttca gcggatattt gtaataattc aggggacaga gttaattcag acattctgtg
    24421 ttctccacta acgatgtaac attggcaatt gataattcag ggttgttggt atattcacag
    24481 gcaagtgcca ttgacgaaca tccctgcgca aatcgaacag aatcgataaa cggcataccg
    24541 tctacccaac aggaggccag ccccgccatc atggcgtcgc ctgccccagt aacattgatg
    24601 acattcgttt taataggggc cgaccaccca ctttcaccgt tgatatcact gtagtaaacg
    24661 ccatcccctc ccatgctcag caccaggcgg ttcaggccgt gttgatgaaa ccaggcggcg
    24721 acctttgcga catcttcacg tccggaaagt gcaattccgc tcaacgtttc ggcttcaaga
    24781 cggttaggtt ttaacgtatg gattttgctc agatgatcgc ggattttgac gcatttccat
    24841 gccgataccg gatcaataaa tacaggcgtt tccccgctat tttccagaac ccagaccagc
    24901 gcctcttcac taaggttgca atccgcgaca ataacctttg caccgcgaat aaactcctga
    24961 tgctgggcaa gaaattctgc agaaatacag tcactaatac tcatatcatt tatcgcgaca
    25021 agcatttctc ctgtattatc gagcagtgat aaatagctgg atgtattctc acccggaaca
    25081 attaagcatt tatcgacata aacaccggat tgattagttt gcgccagtaa tgattgccca
    25141 taaaaatcac cacctactgc acttaaaagc caggcatttt tccccagtaa cgccagatta
    25201 tgagcgatat tccttcccac gccacctggg gtaaatttaa tttttccggg attcgaatcc
    25261 gcataattca atggatgatg tgaataccca gccacatcaa tattcgccga gccaataatt
    25321 actatgtagt ccttttcgcg catagcacgc cccctggcaa taaattgcca acgaagaaat
    25381 tgggtaaaat agtcattaca gccaataaga ctattagagc acgtgttcag atttaaacac
    25441 atgttcataa taagcatgtt taacgtaatg taaatcgatc agaaaaaacg aaatcggcca
    25501 gatatgaagg cggtcacaaa gtttgtctat ttatgcagca acaaaagaga aagttgtgat
    25561 gtgtaacaca caatatggca ggcaagaaaa agggcagaga tgtttctgcc ctcagattgt
    25621 tgacaacgcg cgctttgttc atgctggatg tggcgtaaac gccttatccg acctacaaaa
    25681 gcgcaaaaat tcaatatatt gcagggttca tgtaggtctg ataagcatag cgcatcaggc
    25741 aattttgcgt ttgtcatcat cggggcagag atgtctcagc cccgatgatg aagagaaatt
    25801 atgcgttttt ttctactatt tgtgtttccg ggcgtttgag gaaggcatag gccaaacccg
    25861 ccaccagcgt accggcaata attgctacca ggtaacccaa taccggcgta atagcgccag
    25921 ggatcagcag aacaaacaga ccaccgtgtg gtgccatcag tttcgcacca atcgccattg
    25981 agattgcgcc agtcagcgcc ccaccaacga tacagcacgg tagcacacgc atcggatcac
    26041 gagcagcaaa cggaattgca ccttccgaaa taaagcacag accaagtacc agagcggctt
    26101 tgccaccttc ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca
    26161 ttgccagcgg tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag
    26221 tactcagcag acccacaccg aatgcgtacg ctgctttgtt taccggaccg cccatgtcag
    26281 tacacatcat gccaccgagg atcgccccca gcagaacagc attcgcagtc cccatggtct
    26341 gcagccagtg agtcaaccca tcgagaatgc cagcaaccgg tttaccgatc aggtaaatca
    26401 tcgccagacc gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagtgcct
    26461 ccatactctg tggcagtttc agttgcgtac tgattaactt cgcaatgtaa cccgccagga
    26521 agcccgcaat aataccacca atgaagccag aaccggtgct aaccgccagc ataccgccaa
    26581 tcagacccgg cgtgagaccc ggacgatcgg caatggagaa ggcaatataa cctgccagta
    26641 ccggcaccat cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg
    26701 tacccggctc tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac
    26761 ctgcaacaac catcggcagc atgtaagaga cgcccgtcag caagtgacgg taagcgcctg
    26821 cactctcttt cttaccttca gtggtcgctg tttgagcttt gcccgctggt tcatacggcg
    26881 ttgcttcagc aaccgcttta tccagttcct gcgcagtttt cttcagcgcc agaccggtag
    26941 aggtgcgata catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca
    27001 caatcaccag atccgctgct gcgacttctt caggggtgat tgcattgccc gcgccaacag
    27061 aaccacgggt ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt
    27121 cagccgccat aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca actacgcgtt
    27181 tcggaccgct ggcggcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa ggtttcgcat
    27241 ggcctttggc ttcactcagg aacaactcag ggtgcgcaac tgcccgggaa atatcgccca
    27301 gccagacatt tttaccgttc agcgcgctgt cattcgggat ggaatcaccg agaacaatcg
    27361 ccatttctgc gtcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc
    27421 ccagcagggt cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca
    27481 gcgttttcat tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc
    27541 attgcggcca actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct
    27601 gcaacagctg tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa
    27661 atcaggccac caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt
    27721 ggtttggcga tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa
    27781 ataacaacat gcgcgatgcc ttgttcacgc agcgcatgcg cagcttcaat cacatctttc
    27841 atttcaggca gtttacggcc tgcccagatt tccagctcgc ggcggttagg tttcaccagc
    27901 cacggtgccg ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa
    27961 ggacactgac tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac
    28021 ggtaagcttc cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc
    28081 acaaagcgtt cccagtcggc gggggtgact tcaaaacccg agaagttgaa gtcggtcact
    28141 tcgccgtctt tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa
    28201 cggttggcaa tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccc
    28261 aggaagccgc caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg
    28321 cctttacccg ccgcatgcag accagtggtt ttcaccaggt tcacttcgcc gcgttcaatt
    28381 tccgggcaga aaccaacaag gtcataagcc ggattaaggg tgatcgtagc aacacgtctg
    28441 ctcattatgc gccctcccca agaccagcag cgatagcgtc gccaatggct ttcagcgcct
    28501 gttcagcatc tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg
    28561 ccacaacttt catcagacta cgtccgtttg ccggtttgcc ggtgccatca aggtttgtca
    28621 cggtaatatc actgttaaat tgtttaatgg tattgaccag catggtacct ggacgagcat
    28681 gcaggccgtg ttcattgcgc accacaaact ccgcgcttaa cacgtcgtcg gtcggcgcat
    28741 catcgctggt cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt
    28801 tattatcgag caacaaatca gcgagacgct taagaaccgc gatgggctga tcgtcattca
    28861 tcgccacact caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac
    28921 ggcttaccgc aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct
    28981 gcccgaggtt cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc
    29041 ccgcctcttt cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcgacga
    29101 tatccaacgt cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca
    29161 gcaatgcgcg aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat
    29221 cgctcagtac gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa
    29281 taccgatcgc cacgtacgct acgtggccgt caccccaggt gacgccttcc gggaactgaa
    29341 atacctgaac gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag
    29401 caataccatt gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt
    29461 agccttcagc tacattaccg gcctgcacca gcgccgcagc gacctggcga atcgcctctt
    29521 ctttgtctcc ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc
    29581 tcctctcttg ctgaattgaa acgattcagc ttctatgaga aaaaagcgcc aacctggctt
    29641 agggttaaag acaagatcgc gctgaaacgt ttcaagaaag cataatactt ttgtttcagc
    29701 acgcaaggaa agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa ttttttgtca
    29761 tttttcctaa ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc
    29821 agccaatcag caacagtgaa atggcatttt gattattcgt ggcgcttttg atttcgctgt
    29881 ctgtttattt tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt gatcgaccag
    29941 catgcataac tcccccgcag tcaccagcgc gaaatcgttt gacctgacct cgatggcgtt
    30001 tttaatcgtt gcctttctca ccggtattgc gggcgctctg caaaccccga cactcagtat
    30061 ttttcttact gatgaagtac atgcccgtcc ggcgatggtg ggattcttct ttaccggcag
    30121 cgcagtcatt gggattctgg tgagtcagtt tctcgccggg cgctctgata agcgcggcga
    30181 tcgcaaatcg ctgattgtct tttgctgcct gttaggcgtg ctggcctgca ccctttttgc
    30241 ctggaatcgc aactactttg ttttgctatt cgttggcgtc tttcttagca gctttggctc
    30301 aaccgctaac ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga
    30361 ggcggtgatg ttcagctctt ttttacgcgc tcaggtttca ctggcatggg tcattggccc
    30421 accgctggct tatgccttag cgatgggttt cagctttacg gtaatgtatc tgagcgcagc
    30481 ggtagcgttt attgtttgcg gcgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga
    30541 gcttccgctg gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg atacgttgct
    30601 gctgtttgtc atttgtacat tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc
    30661 gctatttatt atcaacgaac tgcatcttcc cgagaaactg gccggtgtga tgatggggac
    30721 cgccgccggg ctggaaatcc cgaccatgtt gattgccgga tattttgcca aacgtctggg
    30781 taagcgcttc ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg caggaatgct
    30841 gatggcgcat tcacctgtca ttctgttggg cttgcagctg ctaaatgcta tttttattgg
    30901 cattctgggc ggtatcggga tgctctattt tcaggatttg atgcccggtc aggcaggttc
    30961 agccaccacg ctctatacca acacgtcgcg cgtgggctgg atcatcgcag gatcagtggc
    31021 gggcatcgtc gccgagatct ggaattatca cgctgtgttc tggtttgcga tggtgatgat
    31081 tatcgccact ctgttttgct tactgcgaat taaagatgtt taaggcgcgg tcaacatttc
    31141 cagatcgata agccaggtca ttgcctgctg acgcgagtta ccgcacattt cagcgctggc
    31201 ttgtaagcct gcacagactt tcggtcgcag tggcgaggtg aaaattttac agcgttgctg
    31261 ttcatcaagc tgaatgcagg gcgtattggc gggcttgcca tctggcatac cgggaatagg
    31321 gctggaaatt gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg
    31381 ttctcttaag gttaaccacc ggattgatga gcgcgcacag taccaccttt tttgcaccag
    31441 caaaagtgcg aataccactt gcccgaaaga cccgtcgcga gtactttgtc gcgatatttt
    31501 tgacattttc gactacagga atttttcgat gccaagagcg aacgaaatta aaaaaggtat
    31561 ggtactgaat tacaacggca aactgctgct ggttaaggac attgatattc agtcgcccac
    31621 tgcccgcggt gccgctacgc tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa
    31681 agtagaagag cgtttcaaag gtgatgatat cgttgacacc gtgacgctga cccgccgtta
    31741 cgttgacttc tcctatgtcg atggtaacga atatgtcttt atggataaag aagactatac
    31801 cccgtatacc ttcaccaaag atcagattga agaagagttg ctgtttatgc ctgagggcgg
    31861 catgccggac atgcaggtgc tgacctggga cggtcaattg ctggcgcttg agctgccgca
    31921 gaccgttgat ctggaaatcg ttgaaactgc accaggcatc aaaggggcat ctgccagcgc
    31981 ccgtaacaaa ccggcgacat tgagcactgg tctggtgatt caggtaccag aatacttaag
    32041 cccgggcgaa aaaattcgta tccatatcga agaacgccgt tatatgggtc gtgctgacta
    32101 acttcagccg catgcagaaa agggatagct caggctgtcc cttttttaat ttattatacc
    32161 aatcttctat tttgcgcttc ctgaacacct tacgcccgca attttcactc gttgattgat
    32221 cgccctcaca cttcatcgca ttaacaatcc agaccaattt caattgctgt catataactt
    32281 tacactgtca ttgttaatta atcgttacta agacgtgact cctatgaata caatcgcctc
    32341 cgttacgctc ccgcatcatg tacactctcc acgctacgat cgacagcagt tgcaatcacg
    32401 tatcgttcat tttggctttg gagcctttca ccgcgcacat caggcattac tgaccgatcg
    32461 tgtgctgaat gcccagggcg gcgactgggg gatctgtgaa atcagcttgt tcagcggcga
    32521 tcaactgatg agccaactcc gcgcacagaa ccatttatat accgtgctgg agaaaggtgc
    32581 ggacggcaat caggcgataa ttgtcggtgc cgttcacgaa tgtctgaatg caaaactgga
    32641 ttccttagcg gcaattattg agaaattttg cgagccacag gtggcaattg tttccctgac
    32701 gattaccgaa aaaggctatt gtattgaccc ggccaccggt gcactcgaca ccagcaatcc
    32761 gcgtattatt cacgatctac aaaaccctga agaacctcac tccgcgccag gtattctcgt
    32821 cgaagcactg aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga
    32881 caatattccc gacaatggcc atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaacg
    32941 ttcgccagaa ctcgccgggt ggataaaaga acacgtcagt tttccgggaa ctatggtcga
    33001 ccgcattgtt ccggctgcaa ccaacgaatc actggcggaa ataagccagc atctgggggt
    33061 gaatgatccc tgcgcaatta gctgcgaacc gtttatccag tgggtggttg aagataactt
    33121 cgtcgctggg cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc
    33181 atgggaagag atgaaactgc ggatgcttaa tggcagccac tcttttctcg cttatctggg
    33241 ttacctctca ggattcgccc atatcagtga ttgcatgcag gatcgcgcat ttcgccatgc
    33301 cgccagaaca ttaatgctgg atgagcaagc gccgacactg cggattaaag atgtcgattt
    33361 aacacaatat gcggataagt taattgcacg ttttgctaat ccggcactga aacataagac
    33421 ctggcaaatc gcgatggatg gcagccaaaa attaccgcaa cgcatgctgg caggtattcg
    33481 catacatctg gggcgcgaaa cggactggtc gttgctggca ttaggcgttg caggctggat
    33541 gcgttacgtc agcggcgttg atgatgccgg aaatgccatt gatgttcgcg atccgcttag
    33601 cgataaaatt cgtgaacttg ttgcggtcag cagcagtgaa caacgcgtaa ccgccctgct
    33661 ttccctgcgt gaaattttcg gtgatgatct gccagataac ccgcattttg tgcaggccat
    33721 cgaacaagcc tggcaacaaa tcgcacaatt cggcgcgcat caggcgctat taaacaccct
    33781 caaaatttaa cgatttctgc ggttaaagcg gatgaagctc gccttcgtcc gctctcccct
    33841 tctcttttct gcctttttta gccaggatta acgctcagtt aacttaccag agtattgcgg
    33901 agccgttgtg accagaacca acctcatcac cggttttctc ggcagcggga aaactacgtc
    33961 gattcttcat ctgttagccc ataaagatcc caacgaaaaa tgggcggtac tggttaatga
    34021 atttggggaa gtcggaattg atggtgcttt gctcgccgat agcggcgcac tgttgaaaga
    34081 gatccccggc ggctgcatgt gctgcgttaa tggtttaccc atgcaggtag ggttgaatac
    34141 cttactgcgt cagggaaaac cagaccgctt gttgatagag ccgaccgggc tgggccatcc
    34201 gaaacagatc ctcgatctct taaccgcacc cgtctatgaa ccgtggatag atctgcgcgc
    34261 cacgttgtgc attctcgatc cacgcctgct gctggacgaa aaaagcgcca gcaatgaaaa
    34321 cttccgtgac cagctggctg ccgcagacat tattgtcgcc aataaatccg accgtgcgac
    34381 gcccgaaagt gagcaagcgc tagagcgctg gtggcagcaa aatggtggcg atcggcaatt
    34441 aattcacagc gagcatggaa aaattgacgg tcatcttctg gatttgccgc gtcgcaattt
    34501 agccgagttg cccgccagcg ccgcgcattc tcatcagcat agcgtgaaaa aagggttagc
    34561 agcgttaagc ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg gacaagggca
    34621 tcaggcctgt ggctggatat tcgacgctga tacggtattc gacaccattg gtattctgga
    34681 atgggcgcga cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct
    34741 ggtgcgaatc aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc
    34801 ggacagtcgt attgagctga tttccagcag cgaagctgac tggaatgcct tgcagagcgc
    34861 gctgttgaag cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt cagtaagata
    34921 attagagaaa atatgattaa aaatttgccg caaatagtgt tgttgaatat tgtcggcctc
    34981 gcgctgtttc tttcctggta tattcccatt aatcatggat tctggttgcc gattgatgcg
    35041 gatatttttt atttctttaa tcagaaactg gtcgaaagta aggccttttt atggctggtt
    35101 gcattgacca acaatcgcgc tttcgacggt tgttcactgc tggcgatggg aatgttgatg
    35161 ctgagtttct ggctgaaaga aaacgcccca ggcagacgac gtatcgtgat tattggtctg
    35221 gtcatgctat taactgcagt ggtattaaac cagctgggtc aggcactaat tcctgtaaaa
    35281 cgggccagcc caacgttgac ttttaccaat attaaccgcg tcagcgaact gctctctgtt
    35341 cccacgaaag atgcctcacg agatagcttt cccggcgatc acggtatgat gctgcttatt
    35401 ttttcggcat tcatgtggcg ttatttcggc aaagttgcag gccttatcgc ccttattatt
    35461 tttgtggttt ttgcgtttcc cagagtaatg attggcgcac actggtttac tgacatcatc
    35521 gtcggttcca tgaccgtgat attgatcggc ttgccctggg tgttgctgac gccattaagt
    35581 gatcgattaa tcaccttttt tgacaaatca ctacctggaa aaaacaaaca tttccaaaac
    35641 aaataactca cagtaattaa catcatcagg tttattttta tagtgaggat aatcctgatg
    35701 atgcgcctcc tgctttcatc catcgagcgc aaaaatcatt ctctaagtaa atgaatggat
    35761 tgcatgcgtt tcactcaatc gtactttaat tgaccaatcc cgcttattaa ctttctgtat
    35821 cactttttct tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat
    35881 ctcaaactcg ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag
    35941 tccacagatt tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc
    36001 ttggcatttt tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg
    36061 gtcaaatctc aaccgatttt gagatatatc ttgcgcggga tccccgcgat tgcagtagcg
    36121 gttctgcttt ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt
    36181 gcagtgggta gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt
    36241 cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgc
    36301 tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt acagcgtaca
    36361 ttccgtgagc aatttggctt agaacttccg cgttcgactt atgaacagca ggaaatgggt
    36421 aaatctgttt cccgcagtaa tttgcgcacg ggtgatttag ttctgttccg tgccggttca
    36481 acgggacgcc atgtcggtat ttatatcggc aacaaccagt ttgtccatgc ttccaccagc
    36541 agtggtgtta ttatttccag catgaatgaa ccatactgga agaagcgtta caacgaagca
    36601 cgccgggttc tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac
    36661 gagttaactg aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg
    36721 atgtttccgc gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg
    36781 taatttcagg aaattaacgg aatcatgttt atacgcgctc ccaattctgg acgtaagctc
    36841 ctgcttacct gcattgttgc aggcgtgatg attgcgatac tggtgagctg ccttcagttt
    36901 ttagtggcct ggcataagca cgaagtcaaa tacgacacac tgattaccga cgtacaaaag
    36961 tatctcgata cctattttgc cgaccttaaa tctactactg atcggctcca gccgctgacc
    37021 ttagatacct gccagcaggc taaccccgaa ctgaccgccc gcgcggcgtt tagcatgaat
    37081 gttcgtacgt ttgtgctggt aaaagataaa aaaacattct gttcatctgc aactggtgag
    37141 atggacattc cactcaatga attgattccg gcgctcgaca ttaataaaaa cgtcgatatg
    37201 gcgatcttac ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc
    37261 aaccctttgc tgaaaaatag cggcgtgttt gccgctctga atctcaacct gacgccttca
    37321 ctcttttata gttcacggca ggaagattac gatggcctcg ccctcattat tggtaatact
    37381 gcgctatcta ccttttcttc acgtttgatg aacgttaatg aattaaccga catgccggtc
    37441 cgtgaaacta aaattgcggg cattcctctg accgttcggc tttatgcaga tgactggaca
    37501 tggaacgatg tgtggtacgc atttttactg ggtggcatga gtggaacttt cgttggactt
    37561 ctctgctatt acctgatgag tgtgcgtatg cgcccaggca gagaaatcat gaccgccatc
    37621 aagcgcgaac aattttacgt ggtatatcaa ccggtggttg atacacaagc tttgcgggta
    37681 acgggcctgg aagtactgct acgctggcgg catccagtag caggagaaat acccccggat
    37741 gccttcatta actttgccga agcgcaaaag atgattgtgc cactgactca gcacctgttt
    37801 gagttgattg cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg cgtcaaattt
    37861 ggcattaaca ttgcgccggc ccacttgcac agcgaaagct ttaaagcgga tatccagaaa
    37921 ctgctcactt ccctgcccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat
    37981 atgctgaaag agcgagaagc cacacaactc ttcgcctggc tgcactcggt cggcgtagaa
    38041 attgctattg atgacttcgg taccgggcac agcgcgctta tctatcttga gcgttttacg
    38101 ctcgattatc tgaaaattga tcgtggattt atcaacgcca tcggtacgga aacgatcact
    38161 tcacccgtac ttgacgcggt gctgacgctg gcgaaacgtc tcaatatgct gacagttgct
    38221 gaaggggtcg aaacgccaga acaggcacga tggctaagcg aacgcggcgt taatttcatg
    38281 caaggctact ggattagtcg cccgttaccg ctggacgatt ttgttcgctg gctgaagaaa
    38341 ccgtatacgc cgcagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg
    38401 ccggattgcg gctaatgatg agtaaaagga aatccgttgc agatgattgt gcgcatactg
    38461 ctgctgttta tcgctctgtt cacctttggt gcgcaggcgc aggctatcaa ggaaagctat
    38521 gcctttgccg tactgggcga accccggtac gcatttaatt tcaaccattt tgattatgtg
    38581 aaccccgccg cgccaaaagg tgggcaaata acgttgtctg ccctcggcac cttcgataat
    38641 ttcaaccgct atgcactgcg cggcaatccg ggcgcacgca ccgagcagct gtacgacacg
    38701 ctatttacga cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagcgca
    38761 cgctatgctg aagattattc ctgggtggag gtcgctatta atccacgcgc ccgttttcat
    38821 gatggttcgc ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa
    38881 ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcgccgtta
    38941 accgtgcgca ttgagttagc taaacccggc aaagaagata tgctgagtct gttttcgctg
    39001 ccggtatttc cagaaaagta ctggaaagat cacaaactta gcgacccgct cgccacgcct
    39061 ccgcttgcca gtggtccgta ccgcattacg tcctggaaaa tggggcaaaa tattgtctat
    39121 tcccgcgtga aagattactg ggcagcaaac ttaccggtaa accgtggacg ctggaatttc
    39181 gacaccattc gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca
    39241 ggagcctttg atttgcgtat ggaaaacgac gccaaaaact gggccacgcg ctataccggt
    39301 aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat
    39361 acgcgctggc tggcgtttaa tatccaacgt ccggtattca gcgatcgccg ggtccgggaa
    39421 gcgatcactc tcgcctttga ctttgaatgg atgaacaaag cgttgtttta caatgcctgg
    39481 agtcgcacga acagttattt tcagaatacc gaatatgcgg ccagaaatta ccccgacgcc
    39541 gcggagctgg tgcttctggc accaatgaaa aaagatctac cgccagaagt cttcacgcaa
    39601 atctaccagc cgccggtatc caaaggcgat ggctacgatc gtgacaacct gttaaaagcc
    39661 gacaaacttc tcaacgaagc aggctgggtg ttgaagggtc agcaacgcgt taatgtcaca
    39721 acgggtcagc cactcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta
    39781 ttgccgttcc agcacagcct gcaacggctg ggtatcaaca tggacattcg caaggtggat
    39841 aactcgcaaa tcaccaaccg catgcgcagt cgcgactatg acatgatgcc gcgcgtatgg
    39901 cgggcgatgc cgtggcccag ttccgattta cagatttcct ggtcatcgga atatatcaat
    39961 tccacttata atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt
    40021 attgccgcgc agggaaataa agaaaaatta ctgccgttgg gacgagcact ggatcgcgta
    40081 ttaacgtgga attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg
    40141 tgggataaat tctcccaacc cgctgtacgc cctgtttaca gcctgggtat cgatacctgg
    40201 tggtatgacg ttaacaaagc gacgaaactg ccgtcagcca gacaacaggg agagtagatg
    40261 ggcgcttatc tgattcgccg tctgttgctg gtgatcccaa cgctatgggc gattatcact
    40321 atcaactttt tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc
    40381 attgagtttg gcaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt tcgcgccagc
    40441 catgcgcaaa cgggcgtcgg caatatcagc gacagtaatt accgtggcgg acgcggatta
    40501 gatccagaag tgatcgccga gatcactcat cgctacggtt tcgataagcc gatccacgaa
    40561 cgttacttca aaatgctctg ggactacatc cgttttgatt ttggcgacag cctttttcgc
    40621 agcgcctcgg tgctgacgct gattaaagac agtctgccgg tctccatcac cctcggattg
    40681 tggagcacgc tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat
    40741 aatgggagcc gttttgacgt ctggagtagt gcatttatca tcatcggcta cgccattccg
    40801 gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg
    40861 ttccctctgc gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc
    40921 accgattatc tgtggcatat cacgctgccg gtgctggcga cagtgattgg cggctttgcg
    40981 gctctgacca tgctgacaaa aaactcattc cttgatgaag tgcgtaagca atacgtggtg
    41041 acggcacgtg cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt gttccgcaac
    41101 gccatgctgc tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccggc
    41161 tcgctgctga ttgaggtgat gttttcactc aatggcctcg gcttactggg ctacgaagcg
    41221 accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt atattttcac cctgattggc
    41281 ctgctgctga atattgtcag tgatatcagc tatacgctgg tcgatccgcg tatagatttt
    41341 gagggacgct aatgccgcga ctcagccccg tcaatcaggc ccgttgggcg cgttttcgcc
    41401 ataaccgtcg cggctactgg tcgttatgga ttttcctcct cttgtttggt ttgagtttgt
    41461 gttctgaact tatcgccaac gataaaccgt tgctggtgcg ttatgacggc agttggtatt
    41521 tcccgttgtt gaaaaactac agcgaaagcg attttggcgg cccgctggca agtcaggctg
    41581 attatcagga cccgtggctg aaacaacggc tggaaaataa cggctgggta ctgtgggcac
    41641 cgattcgctt tggtgctacc agtatcaact ttgctaccga taagcccttc ccttctccac
    41701 cctcccggca aaactggctg ggaacggatg ccaatggcgg cgatgtgctg gcgcgcattc
    41761 tctatggcac gcggatctca gttctgtttg gcctgatgct gaccctctgc tccagcgtga
    41821 tgggcgtgct ggcgggggcg ctacaaggct attacggtgg taaagtcgat ctctgggggc
    41881 aacgttttat tgaagtatgg tcggggatgc cgacgctgtt tttgattatt ttactttcca
    41941 gcgtcgtaca gcctaacttc tggtggctgt tggcaattac cgtcttgttt ggctggatga
    42001 gtctggtcgg tgtggtgcgg gcggagtttt tacgtacccg taatttcgac tacatccgcg
    42061 cggcgcaggc gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg ttgccaaatg
    42121 ccatggtcgc taccctcact tttttaccgt ttattttatg tagttcgata accaccctga
    42181 cctcactcga tttcctcggc ttcggtctgc cgctcggttc accgtcactc ggtgaactgc
    42241 tgttacaagg gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc ttcttatcgg
    42301 tggcgatatt gttgtctttg ctgatcttta ttggtgaagc cgtccgcgac tcatttgatc
    42361 ctaataaggc ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt gtcggtgggt
    42421 tttcgccatc agcaaaccgt acgtacagta gtaaatgatg tttcactaca gattgcggct
    42481 ggcgaaacgc tggcgctggt aggtgagtct ggttcaggta agagcgttac cgcgctgtca
    42541 attttacgcc tgctcccttg cccgccggtt gaatatctct ccggcgatat tcgttttcat
    42601 ggcgaatcgc tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg taataagatc
    42661 gccatgattt ttcaggagcc gatggtgtcg ttaaatccat tacataccct ggaaaaacag
    42721 ctttatgaag tgctttcact ccaccgcggg atgcgtcggg aagcggctcg tggcgaaatt
    42781 cttaactgcc ttgatcgcgt cggtatccgc caggcggcaa aacggctgac agattatccg
    42841 catcagctct ccggcggcga acgccagcgg gtgatgattg cgatggcgct gttaacgcga
    42901 ccggaattat taattgccga tgaaccaacc accgcgctgg acgtctctgt ccaggcgcag
    42961 attttacagc tgttgcgcga actgcaaggc gagctgaata tgggtatgct gtttattact
    43021 cataacctca gcattgtcag aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc
    43081 tgtgtcgaga aaaataacgc cgctacgcta tttgcctcac ccactcatcc ttacacacaa
    43141 aagctactca acagtgaacc atctggcgac ccggtgccat tgccagaacc tgcctcaacg
    43201 ttgctagatg ttgaacagct tcaggttgcc ttccccatcc gcaaagggat tttgaagcgc
    43261 attgtggatc ataatgtggt ggtgaaaaac atcagtttta cgctacgggc gggtgaaaca
    43321 ctgggtttag tgggcgagtc cggttccggg aaaagcacga cgggactggc gctgctgcga
    43381 ctgattaact ctcagggcag catcgtcttt gacggtcagc cactgcaaaa tttaaatcgc
    43441 cgccagctgt tacctattcg tcatcgcatt caggtggtat ttcaggatcc aaactcctca
    43501 ctcaacccac gactcaacgt tttgcaaatt attgaggaag gcttacgggt tcaccagccg
    43561 acgctttctg ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga agtgggatta
    43621 gatcctgaaa cacgccaccg ttatccggcg gagttctctg gtggtcagcg gcaacgtatt
    43681 gcgattgcca gggcgttaat tcttaagccc tcgctgatca ttcttgatga accaacatca
    43741 tcactcgaca aaacggtgca ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag
    43801 catcaactgg cctatttgtt catcagccac gatttgcacg ttgtccgcgc gttatgtcat
    43861 caggttatcg tactgcgaca aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt
    43921 gccgcaccgc agcaggagta tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg
    43981 attgtagttt gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc
    44041 agcaaattcg tcctggcgat ttacaaacag gcacggctca ccttcacact ccagcaccga
    44101 acatggaact tcgatgtcgc ttaacgcctg gcttagtttg tacatcaccg accatgcatt
    44161 atcaccatcc gggctaagca atttaagcgc caccaggctg ttgtcaccgc acggtccatt
    44221 ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca gaccagcgat agttctgcgg
    44281 cagtcgatgg acgattgaga gttgtaatga agtcacttat tttccccgga agtacatttt
    44341 acttcacaat ttgtttacat caattttaac acatcatcaa caatccgtct ttatacagat
    44401 cgtaaagatt gatgcctcgc ctgcgtggcc tctatggctc tgatttaaat aatatctgcg
    44461 ggcgtggtca cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt
    44521 aactacaatg atgtgacttt ttacataaat tgattttaca taaaataaac atatatcggg
    44581 ggaatgatag atttgtggtt gacatgcatc aacaaaggaa gccttttagc ttcctcgttg
    44641 tgcaatagat caccgttttt tcggccgact ggcgtacaaa cagaagagaa tggagctggt
    44701 tgcgcagaat gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag
    44761 taatgcgcca acaattgccc ctatgccgaa acggaaggtt cctgccagcg aagatgccgt
    44821 tcccgccata tggggaaact catcaagaat gaccgccatc gcattggatg acaccatcga
    44881 cacgcagccc acaaacgccg caacgccaac caccagcgac cagaatccca gccccagcag
    44941 tgcactgata accatccacg ctgccataat aaactgtatc cacaaccccg agcggaacat
    45001 atttaacgcg ccaatacggc ggacgaagcg gctgttaaag atggtcatca cgaacagaaa
    45061 aagaatgttt agcgcaaagt aataaccaaa gttttccggc gcgacgtggt tgatttcaat
    45121 gtagacaaac ggtccggcgc ttaagaatga gaacatcccg gcaaagctga aaccgctggc
    45181 cagcatataa ctcaggacac gcttatgacg gaacagcgcc gcaaagttac caatagtggt
    45241 acgaatgtga aaaggctggc gacgctccgg tggtaaggtt tctttgatca gaaagaagat
    45301 cattgccgaa gccagaatcg ccgccacggc gaggatccag aagatgtaat gccagctcag
    45361 ccacaccagc acccagccgc caactatcgg tgccatcagc ggtgcaatgg tcgtcaccag
    45421 catgacaaac gacatcattc gcgagaactc ttctttcggg tagatatcac gcatcaaagc
    45481 gttgataacc acgctggccg cagccgcagc cagcccgtgg aagaaacgca tcacaatcag
    45541 ctgatcgatg gtttgcgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc
    45601 gccaagaacc accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc
    45661 caacgcaaag cccagaatat aagtactcag ggtcatctgc gtactgcccg ccggtacacc
    45721 aaactgcgct gaaattaccg gtagcgcagg cagatacata tcaatcgaca gcggcatcaa
    45781 catggccagc aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac
    45841 aacgggctcc tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt cttcagttaa
    45901 cggacgatat tcaccggggg ctaaatcagc atccagcgta atgccgccaa tacgttcacg
    45961 atgcagctca accacatggt tacccacggc ggcgaacatg cgtttcacct gatgataacg
    46021 cccttcgctg atggtcagac gaacctgcgt tggggtaatc acttccagca ccgccggctt
    46081 agtgagatct ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc
    46141 agctacgggt gattccagcg ttaccagata ggtcttctcg caatgatggc gcggggaagt
    46201 aatgcggtgc gaccactgac catcatctgt cattagtacc agaccggtgg tatcgatatc
    46261 cagacgccct gcagcgtgca gtttccacgc taccggttca tcaagaaaat agagcaccgt
    46321 tgggtgatcc gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta
    46381 acgtggaccg tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcagggag
    46441 cagtttgaac gctgcattac ggacgatttc gccatcgacg gtgacacgat tgccgcggat
    46501 ttcacgcccg gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg
    46561 catgtgtgtg attttgcctg taaaaaacgg aggtcgggta atgacccgaa aatctgaact
    46621 gttgtctgcc cagtatagcg gtctaataac ttccctcaag ggaaaaagat ccgtggcata
    46681 ctattagcag aataatctac ctacgcgaga ccatgatttt tacactccgc ccatatcagc
    46741 aagaagccgt ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct gctgtaatcg
    46801 tgctgcccac cggtgcaggt aaaagcctgg tgatagcgga actggcgcgg ctggcgcgtg
    46861 gtcgcgtgct ggtgctggca cacgttaaag aactggtggc acaaaaccat gcaaaatatc
    46921 aggcgctggg gctggaagcc gatatttttg ccgccgggct aaagcgcaaa gagagccacg
    46981 gtaaagtggt atttggcagc gtgcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg
    47041 aattttcgct gttgattgtc gatgaatgcc accgtattgg tgacgatgaa gagagccagt
    47101 atcagcaaat cctcactcac ctgaccaaag tgaatcccca cttacgcctg ctggggctga
    47161 ctgccacgcc ttttcgactg ggcaaaggct ggatctacca gttccattat cacggcatgg
    47221 tacgcggcga tgagaaagcc cttttccgtg actgcattta tgagctgccg ctgcgttata
    47281 tgattaaaca cggctatctg acgccgccag aacggctgga tatgccagta gtgcaatacg
    47341 atttcagccg cttgcaggca cagagtaacg gactgttcag cgaagccgat ctcaaccgcg
    47401 agctgaaaaa acagcaacgc attacgccac acatcatcag ccagattatg gagtttgctg
    47461 caacgcgcaa aggggtgatg attttcgccg cgacggtcga acacgcaaaa gagattgtgg
    47521 gattactgcc tgccgaagat gcagcactga ttactggcga cacacccggc gctgagcgcg
    47581 atgtgttaat tgaagatttt aaagcccagc gttttcgcta tctggtcaac gtcgcggtac
    47641 tgaccaccgg atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat
    47701 cggtgagtct ttaccaacaa attgtcgggc gcggcctgcg cctggccccc ggcaagactg
    47761 attgcttaat tcttgattat gcgggtaatc ctcacgatct ctacgcgccg gaagttggta
    47821 caccgaaagg caaaagtgac aacgtcccgg tacaagtttt ctgccctgcc tgcggttttg
    47881 ccaacacctt ttgggggaaa acgactgccg acgggacatt gattgaacac tttggtcgtc
    47941 gctgtcaggg atggtttgaa gatgacgacg gacatcgcga acagtgtgac ttccgtttcc
    48001 gttttaaaaa ttgcccgcaa tgtaacgcag aaaatgatat tgccgcccgc cgttgccgcg
    48061 agtgtgacac cattctggtt gacccggatg atatgttaaa agcggcgcta cgactgaaag
    48121 acgcgctggt attacgctgt agcggcatgt ctttgcagca tgggcacgac gaaaaaggcg
    48181 aatggttgaa aatcacctat tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc
    48241 tgcaaacgcc cgcccagcga actgccttcg agcaactttt tatccgcccg catacgcgca
    48301 caccgggcat cccgctgcgc tggatcaccg ccgccgatat cctcgcccag caagccttat
    48361 tgcgacaccc ggattttgtc gtcgcccgca tgaaaggcca gtactggcaa gtgcgtgaaa
    48421 aagtgttcga ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt
    48481 tttgattgat gtataaggcg attgagtata gaatctcgcc cgcttttgca tacgcaaagc
    48541 agatcactta cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg
    48601 tttactatca acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt
    48661 gccgctaaca agttcccggc aatcatctac ggtggcaaag aagcgccgct ggctgtcgaa
    48721 ctggatcacg acaaagtcat gaacatgcaa gttaaagctg aattctacag cgaagttctg
    48781 accatcgttg ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcatccg
    48841 tacaaaccga agctgctgca catcgacttc gttcgcgctt aattgctgaa taagttgtaa
    48901 aaaaccccgc tccggcgggg ttttttgtat ctgcaaatta tgcctgatta cggtattgct
    48961 attttttgca ggccagataa ggcgttcacg ccgcatctgg cattcagaac acaaaaccga
    49021 ttaattgccg ccagacgtcc ggcgctgcaa ttgatcgcgc aagttcggcg gcgtgccttt
    49081 aatggtcagg gtatcggttg ctggatccca gaagatgcgt tcacctaaca gcatcgcatc
    49141 aaagttaata gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct
    49201 acggtctgcc gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa
    49261 gctcacttcg ctgacgcctg ccagctcttt cgacagcgat tccagttcaa tctcttcacc
    49321 tgcctgcagc tgctcattac agtagctata aacctgctgg cgcacattct gtcgctctgc
    49381 tttatccagt tgtgcttcgg cagtgaaatc atcgaccgct tgcaacagcc cacggttttg
    49441 cgctttcgcg tttaaccctt cgctggcacc gaggaaatcc ataaagaaat ccgccacttt
    49501 gcgccctact cgccctttca ggaaagtgag atagcgggtc gactctggat tggtttccca
    49561 ctcggttaaa tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgggttgg
    49621 gttgatgtcg agattttcgt taacacgcat actgctcagg ttgctcagaa ctgccaccag
    49681 cagatactcc accgccagat agcgatagtg gcaaaacagc acgaatccac cgtcagcaaa
    49741 aggatatttc gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa
    49801 gtcctcttcg ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa
    49861 caagccgtaa gctttatttt tggcgctata gacccgatgc agttctgcca ccatctcaac
    49921 gacggtttcc gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc
    49981 acgcttgata agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc
    50041 tttaagaccg ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc
    50101 attttacaga aggcggcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc
    50161 gttgctacgg taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat
    50221 ttcccgctac agtgatgaac aggttgaaca attgctcgca gagctgctca acgtactgga
    50281 aaaacataag gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct
    50341 tatcaacacc agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc
    50401 cttacagtcc tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg
    50461 taactcatcg tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt
    50521 ttgcactgtt caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg
    50581 actggccgac aacgcttgct ggtcgcattt attcctacgt aagcattatc ggtcatttca
    50641 gcttcctggt gttcgccacc tacttgctga tcctcttccc gctaaccttt atcgtcggct
    50701 cccagaggct gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg acgctattac
    50761 tgatcgacag cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcagc
    50821 tggttatcaa cccagacgaa aatgagatgg cgcgcgactg gcagctaatg ttcatcagtg
    50881 tgccggttat tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca
    50941 gcctgacgcg tcgtcggcgc ttcgcgcgcc cgctggccgc attcttattt atcgccttta
    51001 tcgcctcgca tgtggtttat atctgggccg atgccaactt ctatcgcccg atcaccatgc
    51061 agcgcgctaa cctgccgctt tcgtacccga tgacggcacg acgttttctt gagaagcatg
    51121 gcctgcttga tgcgcaggag tatcaacgcc gtcttattga gcaaggcaat ccagacgccg
    51181 tttccgttca gtatccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg
    51241 tgctgttgat tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc
    51301 tggcaggttt tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc ggcaacacta
    51361 cagacaacgg catctttggc ctgttttatg gcatctcgcc aagctatatg gacggcattt
    51421 tgtcgacccg tacgcctgcg gcgttaatta ctgcgcttaa tcagcaaggc tatcagctgg
    51481 ggttattctc gtcagatggc tttaccagcc cgctgtatcg gcaggcattg ttgtcagatt
    51541 tctcgatgcc gagtgtacgc acccaatccg acgaacagac cgccacgcag tggatcaact
    51601 ggctgggccg ctacgcacaa gaagataacc gctggttctc gtgggtctct ttcaatggca
    51661 caaacattga cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcagcaggca
    51721 atgtcgatga ccagatcaac cgcgtgctca atgcactgcg tgattctggc aaactggaca
    51781 atacggtggt gattatcact gccgggcggg gtattccgct gagcgaagag gaagaaacct
    51841 ttgactggtc ccacggtcat ctgcaggtgc cattagtgat tcactggcca ggcaccccgg
    51901 cgcagcgtat taatgcgctg actgatcata ccgatctgat gacgacgctg atgcaacgcc
    51961 tgctacatgt cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc
    52021 aacgccgcca ttactgggtt accgccgcgg ataacgatac gctggcaatt accaccccga
    52081 aaaagacgct ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa
    52141 gagtgaaaga tgaaaaacca cagttaagtt tgttattgca agtactgaca gacgagaagc
    52201 gttttatcgc taactgatta attataaatc agttagcaaa atatcttact tgcaatcggt
    52261 gtggaaaacg gtagtattag cagccacgag tcggcacgta gcgcagcctg gtagcgcacc
    52321 gtcatggggt gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga
    52381 aaaccaacca attgcggttg gtttttttat atctgcattt aattcgataa acagaccgcg
    52441 acacatcacg gcctgtttat tttctgttat cagaacgtcc agactacacc cgcctgagtt
    52501 gtgctgcttt cttcacagga gaaacgacta tcgataccga gcgtcaggcc cgtcaatgtt
    52561 tgctcaaagc cagctcccgc atcagtgttc acgctgttgc ggctgttaat tgccgaactc
    52621 cacatcgccg ccctttacgc taccaagacg ctcacgcacg gtgtccagtt ctgcatcaaa
    52681 taccagcggt tgtgctgccg ccatattcag cacatccgtg gttgaagggg gcatttgtac
    52741 gcgattctct gtgagactcc agctatggtt gccattatcc agcaaggtat attcatacgt
    52801 actgatatca acaacgcttc cggcattgcc caacgtaaat gccgcatcac cgcgccctcc
    52861 tttaatcgtg ggatcagtcg ccgcgccata aacctgtagc actccattat tgatcgtagt
    52921 ctgatttacc gcgccaccca tttgaacaat ttgataacca tcatttaata ctgcgtattc
    52981 tgcggtgcca tttatttcaa cgtactgata tcccccgtta atttcagtat tactacttac
    53041 accaaattct tatatatgct gttctccgcc aggattgatt ttcgtattat tggcggcccc
    53101 ataaaccagc tggatatctt tttccaggac aacaccatca actgtctcac catactcaac
    53161 agttgatgct aatgctatgg gtgaatataa cgctgtagcc accagaacag atattgcgga
    53221 acgacaaaga gaaacagaac cagattggtg cattaagctt tcatcctatg aaattaattg
    53281 ctgttaaaag cattggggac agaaaatacc catagctcca tacccggagt cagttttaaa
    53341 aaactgttta aagaaatgca caagtattgt gattgatttt ttagttgttt ttcctgatga
    53401 gaagctgatg caaaattccg tctttataat gaagatgatg ccaaagcgaa cgacaaagtt
    53461 gtagttttca ctacatgtcc atacataaaa tggggtaaca ttcacgcgcc tggtagcgtt
    53521 accaacgcta cgctcaaaca taatgattct aataaaacct caggagacta ctatgcctga
    53581 agcaacgcct tttcaggtga tgattgtgga tgatcatcca cttatgcgac gcggtgttcg
    53641 tcagttactg gagcttgatt ctggctttga agtggtcgcc gaagcgggcg acggcgcaag
    53701 cgcgatcgat ctggcgaata gactggatat cgacgtgatc ttgctggatc tcaatatgaa
    53761 aggcatgagt ggcctggata ccctcaatgc cttgcgcagg gatggcgtta ccgcgcaaat
    53821 tattatcctg accgtatcag atgcctccag cgatgtcttt gcactgatag acgcaggcgc
    53881 agacggttat ctgttgaaag acagcgaccc ggaagtgtta ctggaagcga ttcgtgccgg
    53941 agcgaaaggc agcaaagtct ttagcgaacg cgtcaatcag tacttacgtg aacgtgaaat
    54001 gtttggcgcg gaagaagacc ccttcagcgt gctgacagaa cgcgagctgg atgttctgca
    54061 cgagctggca caggggctgt caaataaaca gattgcctcg gtgttgaata tttccgagca
    54121 gacagtaaaa gtccatattc gcaatctgct gcgtaaactc aacgtccgct cacgcgtggc
    54181 ggccaccatt ctgttcctgc aacaacgcgg ggcacaataa aaatagcccg atggcatcac
    54241 catcgggcca ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata
    54301 ctacgttcga tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatttcc
    54361 cacgcggcaa cggcttcgcc aaatcgttgc tgctcaaagg cattaaatgc atacatgctt
    54421 aacacacgga tattgctatg gtccgttctc accaactggc gcagcagttc gccgccgagg
    54481 cggttgtcgt tgggctcaga tgaacgtgtc aacgcttcag cgtatcccag tgcagcatcg
    54541 ctgtttttcg gatcgaggcg atacgcagtg gcgtatgcat cggtggcgat actggcgtta
    54601 cccagcgcca tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt
    54661 tgcagttgag tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg
    54721 gctttcggat ccagtgccct gtccagtaac gccggagcct gtgccgtggc ctgctgccag
    54781 attttcacct gctgataatt gccagtctgg tagtaactga cgccagccac aattaacgcc
    54841 accacaatac ccggcagata aacaatatat ccggcacgct taccttccgg cacgctttgt
    54901 tcaggaaacg cgtccggcac cacgcgtacc cgacgccgcg aacgggcgta aatgacccaa
    54961 ccgccaatgc caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac
    55021 ggcggatcgt aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttc
    55081 ttacttttgc cttcctgcat cagttcatac actttctggc gcaggtcggt ggcaatcatc
    55141 gagttggaat cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc
    55201 tgacggaact gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc
    55261 gctgagccgg agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct
    55321 tactgtattt ctcccacagt ggcttgatct cttcttccca gacgcgagga ttgagatcgc
    55381 ccgcatggcg atagcgaatg atgccgttgc cgtcaataag gaacgtttct ggcgcgccat
    55441 agacaccgag atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat
    55501 tgcccagctc tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga
    55561 ccacgcggat gccctgcgca gaaagctgat tcagatattg atgttccgca cggcaggtcg
    55621 gacaccaggt cgcccagacg ttaagcagca ccggtttgcc ctgagtcagc acatccgcct
    55681 gataaaactg ccccggattg tccagtgatt cgaggcgaaa cttcggcact ggcttgccaa
    55741 tgagcgccga ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca
    55801 gcagcgccgc ggcaatcgcc aggaagataa tcaacggaat taacaatacc ttgcgcttca
    55861 tgcagcctcc ggcgcagttt tctgcgggtt cacgcgctta cgatagcgag gatcaaacag
    55921 acacagcagt ccgcccaaca ccatcatcag cccgcccgcc caaatccagc gaacaaatgg
    55981 tttgtagtaa agacgcacgg cccacgcgcc gttttccagc tcttcaccga gtgccgcgta
    56041 caggtcacgc gtgatgccac cgtcaatcgc cgcttcggtc atcatcgacc cggcagtgtt
    56101 gtaataacgt ttttctgcat acagcactgt ttccggcttg ccatctcgcg ttacgccgat
    56161 agtcgccaca ccgccacgcc agttcggacc ggtcacctct ttgacatcac ggaaggtgaa
    56221 gcgatattca tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct
    56281 atagttctga ctaaaagcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac
    56341 catcccccaa taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc
    56401 aattgccagc accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac
    56461 tttgctttcg aacagccacg gcaacagcag cgacagcacc agcgtagaga tgaaggcgat
    56521 aatcaacaaa ttgcggattt tacgtgggcg atcgcgtccc cagcgcacca gagggccgac
    56581 accaagcagt agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc
    56641 gccaatcgaa atactgccca gtcccagttg cttgtgcacc agcggcagca acgtccccag
    56701 caacaccacc agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg
    56761 cgaccacagc gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag
    56821 cagcgaaccg ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc
    56881 agacgcaaac gcgtgtaccg ataccagcac gccggaacgc accaggaaag tccccagtag
    56941 acacaacgag aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg
    57001 ttcagtgacc gccagtgagt gcatcagcgc agtccccacc agccacggca taaacgaggc
    57061 gttttctacc ggatcccaga accaccagcc gccccagccg agttcgtaat aggcccatgc
    57121 ggaaccgagc acaatgccga gcgtcaggaa gatccacgca gccagcgtcc acggacgagt
    57181 aaaacgcgca taagtgctgt ccagacgccc actcaacaaa gatgcaatgg caaaagcaaa
    57241 cgccaccgag aagccgacgt accccatata aagcagaggc ggatggaaga tcagccccgg
    57301 atcctgtaac agcgggttaa gatcgcgacc ttcaatcggg aagttcggca acgtgcgtga
    57361 gaacgggtta gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat
    57421 cgccagtacg cgggcaacaa tatccagcgg aatacgctga ctaaaaatcg ctaccgcaaa
    57481 ggtccagccg ctcatcagca gcacccacag caggagcgag ccttcatgcg cgccccaggt
    57541 agccgccacg cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac
    57601 ggtaaagtcg ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat
    57661 aaacagcagc caggcaaaca agcgggaaga cgccatcatg cgcgcgtccc cgcgcgccac
    57721 gccccatagc ggatacacgg acagcagcag cgcaattcct agcgccagac acagcagccc
    57781 gttaccaatt tctggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg
    57841 ttagcttcca tcgctttctc aacttctggc ggcgtgtagt tttcatcgtg tttcgccagc
    57901 acttctttcg cgaggatatg attgccttct tccagttcgc cctgcaccac aacgccctgc
    57961 ccttcacgga acagatccgg caaaatgcct tcgtaagaga catctactga accttcagcg
    58021 tcgtaaatgg tgaaagttac ttttagcgaa ttgggatcgc gctgcacact acccggcatc
    58081 accatcccgc caacgcgcag acgctgaccg acttccggca tttgctgagt ttcacgcttg
    58141 ccgtagagaa tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc
    58201 accagaccga tagtcagcgc caaccctgcc agaacggcac aggcaatcca caagcggttt
    58261 ttacggcgaa tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg
    58321 cccgctgttg cgccacacca cgcagaattg cgcgatgctg catcaccgag tgcacgacca
    58381 aaaccaccag cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc
    58441 ccattgcgaa aaattcattc caggaagcaa atgtaggggt catttacggc ctcttttcag
    58501 tatcagttca ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg
    58561 catcagcgtc agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga
    58621 acgcatcgcc ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca
    58681 ccactccacg gagtaatgaa taatcggcag attcaccacg ccaatcagca ccagaatacc
    58741 tgccgcacgg cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tgacaccaac
    58801 atacagaaac agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc
    58861 ccacatcggt tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc
    58921 aatgggggcc atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat
    58981 aaacgctgcc accgccattg atgcataaat gcccatcgac cagatcgccg caggtacatg
    59041 caagtagata atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc
    59101 ccagatccag ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca
    59161 gccacagatt tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt
    59221 ttcgatacca gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac
    59281 gctgccgccg tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt
    59341 gccagatacc catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaaa
    59401 atgagtaatg ggatagtcag cggtaacacc agtatactga gcagcacacc accgcgctta
    59461 agtcccactg tcagcgccac gcccggcgca ccgagaaagc caagcgtagg ggttcccagc
    59521 agcagcgtca gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc
    59581 aggggcgaaa ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc
    59641 agcacaacgg cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc
    59701 aaatcgtcac ggaacagtcg ctcgagtgcc agcaaggatg aaagcagcgc ggcaacccag
    59761 ataatgcccg gcgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa
    59821 agggtaatta caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac
    59881 gctacgcgca gctcaagacg gaaaatgcgc cagaacatca tgcggccccc gtttgcgtca
    59941 gtgaaatgcg gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca
    60001 caatcccccc ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa
    60061 cgtcaatcgc ggtaaaaggc tcgtcgagta tccataacgt ggcacgggtc agccacagac
    60121 gcgctaaagc gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga
    60181 atccggcaag cccggcctgc gccagggctt ccagacattg tgcggtatcg ccatcgcgat
    60241 gataaaagtg cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta
    60301 tccataacag gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga
    60361 gaacctcgcc tgcatcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc
    60421 ccgcgccgtt gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgataaac
    60481 cactaaataa ggttcgttca tcccgctcgc aaagtaactc tctggcttca agcataccca
    60541 cgcaataacc ctgttaaaaa cctggctcga cttcacgcat gtcgggcagc ttgtgcgcta
    60601 tccctttatg gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg
    60661 cggcaaccga tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact
    60721 cctgcgagtt gttgtccttc atgcgccgcc actcattctg tgccatcgtc aggcgatgag
    60781 cttcaaattt ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg
    60841 ctttgagctt gcgtatcatc tttggcacaa actcgtgcgg aacgtgacaa tccggacagg
    60901 tcgcacggac gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg
    60961 tgttacgcat ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg
    61021 tgttaaagcc gccccagaag acgatgccgc cgacaaaacc gatcaacagc agcgtcccta
    61081 gcgccagacg gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac
    61141 ggtcagaatt tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat
    61201 tccccacgat tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac
    61261 gcggagccac ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactaatgc
    61321 gcggcgcgcc agtggtgcga taactttcga caccgtggca ctgcaagcag cggttggtat
    61381 tggtcgttac ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt
    61441 tcagcggcat ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata
    61501 cttccggcga ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca
    61561 gggccagcat cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt
    61621 cgctcccgaa ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca
    61681 gcgaccgcac gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac
    61741 cggggcacgt agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca
    61801 tttctgccga tcggtggccg caacggtaat cacacctttg ctacccagca cgccatacag
    61861 tgcaccaacc ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa
    61921 aaacagcgcg agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat
    61981 cagagaaacg gggttgatcc attcccagat gagcgtgcca gttaaggccg atcccaccag
    62041 aatgaccacc aacagcacgt agcgaatatg gcgagggatc gtcgcagact gattgaggtc
    62101 aaatcgtctg cgtaaccaat ttgctaagtc ggtaatcggg ttcagcggac agacccagct
    62161 gcaaaataat cgctttcctg ccagggcgta gagcacggtg ataatcaccg caccggtcaa
    62221 cgccaccgtg gcaggcagat gaccgctggc aagactttgc agtgtcatca gcggatcggt
    62281 cagtggaacg gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc
    62341 aaaccacgga ccgctcaaaa acatccccag cacgaagaat tgacaaaggc gacgtaacac
    62401 taaccaacgg tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg
    62461 tttacgattt gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc
    62521 ctttcgccag tgacagcggt aacaccttga ttgccggttg ttccagcacg cacacttttt
    62581 cgcacttacc gcagccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct
    62641 taccggttcg cgtgttgcgc tccagctcca gggtgatggc ctcatcaatt ttcgggcatt
    62701 cgcgataaca aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca
    62761 gtaccgccag ccccatccgt gcgtcgtcga tcgattcgat ctcacgatcc agcgcaccgc
    62821 tcgggcacac tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga
    62881 caaaatatgg cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt
    62941 aagggcaagc ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt
    63001 ttatggctcc gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc
    63061 ccagcgccac acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc
    63121 ggcggcgacc attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt
    63181 tgaccgcgca cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca
    63241 gtttgttaac cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgcggcggac
    63301 ggttacgacc gcgcgtttcg acaatcgaga tcacttcgcc acggcgagaa accactttca
    63361 ctttgtcgcc acgacgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaggaccg
    63421 cttcagggaa ggcgcggtgc agttccggta cacggcgagt catactgccg gtatgccagt
    63481 gctccaggac gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg
    63541 ccggttcgaa cggcagcgcg aagatcaccg ctttgccatc cggtttaccg tagaacttgt
    63601 agccttcgcc cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgcgtttctt
    63661 taccgttcac caccggccag cgcagaccgc gtgctttgtg gtagtcatcg aacggcgcga
    63721 gatcgtgacc gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat
    63781 agaagcccag ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gatagcggga
    63841 atttgctcac ttccggagtg gcatacagaa cctcatacag cgttttgcca cgcagttcag
    63901 gtttcttcgc cagcaactct tccggccaca cttcttcagt tttgaagcgg cgggagaact
    63961 gaactaactg ccagagatcc gatttcgctt cacccggtgc ctgtacctgc tgacgccaga
    64021 actgagtacg gcgttcggca ttaccgtaag cgccctcttt ctctacccac attgcggtcg
    64081 gcaggatcaa gtccgctgcc agcgcactga ccgtcggata cggatcggaa acgatgataa
    64141 agttgcgcgg atcgcgccag cccggcatac gctcttcatt aatgttcggt ccggcctgca
    64201 tgttattggt acacatggtc cagtaaacat tcagtttgcc gtctttcagc gcacggtctt
    64261 gtgccaccgc gtgcagaccg attttcgccg gaatggtgcc actcgggata ttccacttct
    64321 tctcgcaaat atcacgatgt ttctcgttag tcaccaccat gtccgcaggc agacggtgag
    64381 caaaggtgcc cacttcacgc gcagtaccac acgcggaagg ctgcccggtc agggagaacg
    64441 gaccgcaacc cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt
    64501 tggcccatac accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct
    64561 tgttcggatc ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca
    64621 tttcggcggt tttttccagc gtatattcag caacgaaggc tttgtaatct tcaaagctca
    64681 ttggttcgga ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac
    64741 gtaaaccgta gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgtttg ctgaagaagt
    64801 cctgatttat cgcattgttt tgaatgatat agttggcgat atagttcagg atcaccaggt
    64861 cagattgcgg cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg
    64921 tagaaagcac cgccacggtg acgttctggt tagaaagacg acggttagtg atgcgcgacc
    64981 agaggatcgg gtgcatctcc gccatgttgg agccccacag cacaaacgcg tcagcctgct
    65041 cgatgtcgtc gtagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacga
    65101 ccgctgacgc catacagtga cgcgcgttcg ggtcgatgtt gttagaacgg aagcccgctt
    65161 tgaacaactt ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac
    65221 cgatcgattc cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga
    65281 aggcctgatc ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca
    65341 tacgcagcag cggctgcgtc aaacggtctt taccgtacat gattttgggc aggaaatagc
    65401 ccttaatgca gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca
    65461 cacgcccctg ctgcgttccg accagaacgc cgcaacccgt accgcagaaa cggcacggcg
    65521 ctttatccca tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc
    65581 tgagaccggc agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac
    65641 tgagtttcat ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc
    65701 cagcacgccc tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc
    65761 tgcttccacc accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc
    65821 ggtaaaggcg ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac
    65881 caggctgcaa acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg
    65941 gacagttggc tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcggctggt
    66001 agatcccgga gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc
    66061 ggcggcattc aactgactga tacgccaggc acgcgtcccc aatggtgaac tgcaaatccc
    66121 aggccctggt gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac
    66181 agaagctgca ctcgttattt ttgaaattaa cgctcggata gccacccgcg ccgcgttgca
    66241 gaatgttgtt ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat
    66301 gagattcatc accggaccag ggcggacgga taccgttact ggctttgcgc cagcgaccag
    66361 tgagtatgcc ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc
    66421 tgaaaattga gagcgaccaa ataaaccgca taattaataa gccattttta tagccgctaa
    66481 gatattaaag gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc
    66541 aaaagagtgg cgggaagcgt ggcaaaaacg g
//
LOCUS       DAZKHK010000008        65248 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKHK010000008 DAZKHK010000000
VERSION     DAZKHK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.008"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..506)
                     /gene="folK"
                     /locus_tag="ACHOS5_000531"
     CDS             complement(27..506)
                     /gene="folK"
                     /locus_tag="ACHOS5_000531"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP9775411.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW"
     gene            complement(503..1900)
                     /gene="pcnB"
                     /locus_tag="ACHOS5_000532"
     CDS             complement(503..1900)
                     /gene="pcnB"
                     /locus_tag="ACHOS5_000532"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP9775412.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1960..2856)
                     /gene="gluQRS"
                     /locus_tag="ACHOS5_000533"
     CDS             complement(1960..2856)
                     /gene="gluQRS"
                     /locus_tag="ACHOS5_000533"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP9775413.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2923..3378)
                     /gene="dksA"
                     /locus_tag="ACHOS5_000534"
     CDS             complement(2923..3378)
                     /gene="dksA"
                     /locus_tag="ACHOS5_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP9775414.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3556..4260)
                     /gene="sfsA"
                     /locus_tag="ACHOS5_000535"
     CDS             complement(3556..4260)
                     /gene="sfsA"
                     /locus_tag="ACHOS5_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9775415.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4275..4805)
                     /gene="thpR"
                     /locus_tag="ACHOS5_000536"
     CDS             complement(4275..4805)
                     /gene="thpR"
                     /locus_tag="ACHOS5_000536"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP9775416.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPDAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4879..7308
                     /gene="hrpB"
                     /locus_tag="ACHOS5_000537"
     CDS             4879..7308
                     /gene="hrpB"
                     /locus_tag="ACHOS5_000537"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP9775417.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQTLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWMDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMATLGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7402..9930
                     /gene="mrcB"
                     /locus_tag="ACHOS5_000538"
     CDS             7402..9930
                     /gene="mrcB"
                     /locus_tag="ACHOS5_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP9775418.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10150..12408
                     /gene="fhuA"
                     /locus_tag="ACHOS5_000539"
     CDS             10150..12408
                     /gene="fhuA"
                     /locus_tag="ACHOS5_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP9775419.1"
                     /translation="MAPSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDQEALKASPCLSIPQSQWGHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSE
                     RDDKEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12459..13256
                     /gene="fhuC"
                     /locus_tag="ACHOS5_000540"
     CDS             12459..13256
                     /gene="fhuC"
                     /locus_tag="ACHOS5_000540"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP9775420.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13256..14146
                     /gene="fhuD"
                     /locus_tag="ACHOS5_000541"
     CDS             13256..14146
                     /gene="fhuD"
                     /locus_tag="ACHOS5_000541"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP9775421.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLTHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMNALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14143..16125
                     /gene="fhuB"
                     /locus_tag="ACHOS5_000542"
     CDS             14143..16125
                     /gene="fhuB"
                     /locus_tag="ACHOS5_000542"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP9775422.1"
                     /translation="MSKRIALFPALLLALLVVAASALTWMNSSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSLGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16160..17440)
                     /gene="hemL"
                     /locus_tag="ACHOS5_000543"
     CDS             complement(16160..17440)
                     /gene="hemL"
                     /locus_tag="ACHOS5_000543"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP9775423.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17606..17653
                     /gene="yadX"
                     /locus_tag="ACHOS5_000544"
     CDS             17606..17653
                     /gene="yadX"
                     /locus_tag="ACHOS5_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP9775424.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17665..19086
                     /gene="clcA"
                     /locus_tag="ACHOS5_000545"
     CDS             17665..19086
                     /gene="clcA"
                     /locus_tag="ACHOS5_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP9775425.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASARENT"
     gene            19110..19175
                     /gene="yadW"
                     /locus_tag="ACHOS5_000546"
     CDS             19110..19175
                     /gene="yadW"
                     /locus_tag="ACHOS5_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP9775426.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19168..19512
                     /gene="erpA"
                     /locus_tag="ACHOS5_000547"
     CDS             19168..19512
                     /gene="erpA"
                     /locus_tag="ACHOS5_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP9775427.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19559..20182)
                     /gene="yadS"
                     /locus_tag="ACHOS5_000548"
     CDS             complement(19559..20182)
                     /gene="yadS"
                     /locus_tag="ACHOS5_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP9775428.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIMLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20220..21020)
                     /gene="btuF"
                     /locus_tag="ACHOS5_000549"
     CDS             complement(20220..21020)
                     /gene="btuF"
                     /locus_tag="ACHOS5_000549"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP9775429.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21013..21711)
                     /gene="mtnN"
                     /locus_tag="ACHOS5_000550"
     CDS             complement(21013..21711)
                     /gene="mtnN"
                     /locus_tag="ACHOS5_000550"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9775430.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21795..23312
                     /gene="dgt"
                     /locus_tag="ACHOS5_000551"
     CDS             21795..23312
                     /gene="dgt"
                     /locus_tag="ACHOS5_000551"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP9775431.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23442..24866
                     /gene="degP"
                     /locus_tag="ACHOS5_000552"
     CDS             23442..24866
                     /gene="degP"
                     /locus_tag="ACHOS5_000552"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP9775432.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGLGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25021..26178
                     /gene="cdaR"
                     /locus_tag="ACHOS5_000553"
     CDS             25021..26178
                     /gene="cdaR"
                     /locus_tag="ACHOS5_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP9775433.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26232..26618)
                     /gene="yaeH"
                     /locus_tag="ACHOS5_000554"
     CDS             complement(26232..26618)
                     /gene="yaeH"
                     /locus_tag="ACHOS5_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP9775434.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26930..27754)
                     /gene="dapD"
                     /locus_tag="ACHOS5_000555"
     CDS             complement(26930..27754)
                     /gene="dapD"
                     /locus_tag="ACHOS5_000555"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP9775435.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27785..30457)
                     /gene="glnD"
                     /locus_tag="ACHOS5_000556"
     CDS             complement(27785..30457)
                     /gene="glnD"
                     /locus_tag="ACHOS5_000556"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP9775436.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHQQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLIQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30519..31313)
                     /gene="map"
                     /locus_tag="ACHOS5_000557"
     CDS             complement(30519..31313)
                     /gene="map"
                     /locus_tag="ACHOS5_000557"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9775437.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31681..32406
                     /gene="rpsB"
                     /locus_tag="ACHOS5_000558"
     CDS             31681..32406
                     /gene="rpsB"
                     /locus_tag="ACHOS5_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9775438.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32541..33392
                     /gene="tsf"
                     /locus_tag="ACHOS5_000559"
     CDS             32541..33392
                     /gene="tsf"
                     /locus_tag="ACHOS5_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9775439.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33539..34264
                     /gene="pyrH"
                     /locus_tag="ACHOS5_000560"
     CDS             33539..34264
                     /gene="pyrH"
                     /locus_tag="ACHOS5_000560"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9775440.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34414..34971
                     /gene="frr"
                     /locus_tag="ACHOS5_000561"
     CDS             34414..34971
                     /gene="frr"
                     /locus_tag="ACHOS5_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9775441.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35063..36259
                     /gene="ispC"
                     /locus_tag="ACHOS5_000562"
     CDS             35063..36259
                     /gene="ispC"
                     /locus_tag="ACHOS5_000562"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP9775442.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKMMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36448..37206
                     /gene="ispU"
                     /locus_tag="ACHOS5_000563"
                     /gene_synonym="uppS"
     CDS             36448..37206
                     /gene="ispU"
                     /locus_tag="ACHOS5_000563"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP9775443.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37219..38076
                     /gene="cdsA"
                     /locus_tag="ACHOS5_000564"
     CDS             37219..38076
                     /gene="cdsA"
                     /locus_tag="ACHOS5_000564"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9775444.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38088..39440
                     /gene="rseP"
                     /locus_tag="ACHOS5_000565"
     CDS             38088..39440
                     /gene="rseP"
                     /locus_tag="ACHOS5_000565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP9775445.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39470..41902
                     /gene="bamA"
                     /locus_tag="ACHOS5_000566"
     CDS             39470..41902
                     /gene="bamA"
                     /locus_tag="ACHOS5_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP9775446.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42024..42509
                     /gene="skp"
                     /locus_tag="ACHOS5_000567"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42024..42509
                     /gene="skp"
                     /locus_tag="ACHOS5_000567"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP9775447.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42513..43538
                     /gene="lpxD"
                     /locus_tag="ACHOS5_000568"
     CDS             42513..43538
                     /gene="lpxD"
                     /locus_tag="ACHOS5_000568"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9775448.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43643..44098
                     /gene="fabZ"
                     /locus_tag="ACHOS5_000569"
     CDS             43643..44098
                     /gene="fabZ"
                     /locus_tag="ACHOS5_000569"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9775449.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44102..44890
                     /gene="lpxA"
                     /locus_tag="ACHOS5_000570"
     CDS             44102..44890
                     /gene="lpxA"
                     /locus_tag="ACHOS5_000570"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP9775450.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44890..46038
                     /gene="lpxB"
                     /locus_tag="ACHOS5_000571"
     CDS             44890..46038
                     /gene="lpxB"
                     /locus_tag="ACHOS5_000571"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP9775451.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46035..46631
                     /gene="rnhB"
                     /locus_tag="ACHOS5_000572"
     CDS             46035..46631
                     /gene="rnhB"
                     /locus_tag="ACHOS5_000572"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9775452.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46668..50150
                     /gene="dnaE"
                     /locus_tag="ACHOS5_000573"
     CDS             46668..50150
                     /gene="dnaE"
                     /locus_tag="ACHOS5_000573"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9775453.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50163..51122
                     /gene="accA"
                     /locus_tag="ACHOS5_000574"
     CDS             50163..51122
                     /gene="accA"
                     /locus_tag="ACHOS5_000574"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9775454.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51220..53361
                     /gene="ldcC"
                     /locus_tag="ACHOS5_000575"
     CDS             51220..53361
                     /gene="ldcC"
                     /locus_tag="ACHOS5_000575"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP9775455.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53418..53807
                     /gene="yaeR"
                     /locus_tag="ACHOS5_000576"
     CDS             53418..53807
                     /gene="yaeR"
                     /locus_tag="ACHOS5_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9775456.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53872..55185
                     /gene="tilS"
                     /locus_tag="ACHOS5_000577"
     CDS             53872..55185
                     /gene="tilS"
                     /locus_tag="ACHOS5_000577"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9775457.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQVFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLSDDLAHCQTQQGTLKIAPMVAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAVGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55219..55479)
                     /gene="rof"
                     /locus_tag="ACHOS5_000578"
     CDS             complement(55219..55479)
                     /gene="rof"
                     /locus_tag="ACHOS5_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP9775458.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLALELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55466..55666)
                     /gene="yaeP"
                     /locus_tag="ACHOS5_000579"
     CDS             complement(55466..55666)
                     /gene="yaeP"
                     /locus_tag="ACHOS5_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP9775459.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55832..56377
                     /gene="yaeQ"
                     /locus_tag="ACHOS5_000580"
     CDS             55832..56377
                     /gene="yaeQ"
                     /locus_tag="ACHOS5_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP9775460.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56374..56796
                     /gene="arfB"
                     /locus_tag="ACHOS5_000581"
     CDS             56374..56796
                     /gene="arfB"
                     /locus_tag="ACHOS5_000581"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP9775461.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56810..57520
                     /gene="nlpE"
                     /locus_tag="ACHOS5_000582"
                     /gene_synonym="cutF"
     CDS             56810..57520
                     /gene="nlpE"
                     /locus_tag="ACHOS5_000582"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP9775462.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(57676..58500)
                     /gene="yaeF"
                     /locus_tag="ACHOS5_000583"
     CDS             complement(57676..58500)
                     /gene="yaeF"
                     /locus_tag="ACHOS5_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP9775463.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(58553..60271)
                     /gene="proS"
                     /locus_tag="ACHOS5_000584"
     CDS             complement(58553..60271)
                     /gene="proS"
                     /locus_tag="ACHOS5_000584"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9775464.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60382..61089)
                     /gene="tsaA"
                     /locus_tag="ACHOS5_000585"
     CDS             complement(60382..61089)
                     /gene="tsaA"
                     /locus_tag="ACHOS5_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP9775465.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61086..61490)
                     /gene="rcsF"
                     /locus_tag="ACHOS5_000586"
     CDS             complement(61086..61490)
                     /gene="rcsF"
                     /locus_tag="ACHOS5_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP9775466.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61608..62423)
                     /gene="metQ"
                     /locus_tag="ACHOS5_000587"
     CDS             complement(61608..62423)
                     /gene="metQ"
                     /locus_tag="ACHOS5_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP9775467.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62463..63116)
                     /gene="metI"
                     /locus_tag="ACHOS5_000588"
     CDS             complement(62463..63116)
                     /gene="metI"
                     /locus_tag="ACHOS5_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP9775468.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63109..64140)
                     /gene="metN"
                     /locus_tag="ACHOS5_000589"
     CDS             complement(63109..64140)
                     /gene="metN"
                     /locus_tag="ACHOS5_000589"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP9775469.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAHKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64328..64900
                     /gene="gmhB"
                     /locus_tag="ACHOS5_000590"
     CDS             64328..64900
                     /gene="gmhB"
                     /locus_tag="ACHOS5_000590"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9775470.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15391 a        16192 c        17971 g        15694 t
ORIGIN      
        1 tatatttata ttcacacgaa aaataattac cacttatcta attcattaaa gccttttgcc
       61 tgtagagctt caactaatga taagccatca ggaaatacca attccggcgc aatttcaaac
      121 agtggccaca gcataaagcc gcgatttttc atatcgtagt gcggaacggt cagacgttcg
      181 gtatttatta cttcattacc aaacagcatg atgtcgagat ccagcgtgcg cggtccccag
      241 cgttcagctt tacggacgcg accttgctgc aattcgatac gttgtgtgtg attgagtagc
      301 tcttcagggg caagagtggt tttcagcgcg acggcggcat ttaagtaatc gggttgatct
      361 tgcggcccca gcggtggggt gcggtaaaac gaagaaacag caagaatgcg gctttctgga
      421 atatcgccta atgctttcag ggcagcattg acctgctcca gcggagaggc cagattgctg
      481 cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg cgtttgcgtg
      541 gacgacgagt acgacgacgc ggtgacggtt cttcatccag ttcgttaagc atcccttttt
      601 ggtctggtgg cgcggaaacc tggaactcac cccaccattt caccagacgt tgcagttcag
      661 cgttacgctc aacttcggct cgtaaggcca acaggtcgta tgccgcacgg aacttaggat
      721 gctccagcag tttccatgcg cgttttccct gacgacggga catacgcaac tgcaactgcc
      781 agatatcgcg ggttagcgtg gtcaaacgtt tcgggatcgc cagtgaacgg caggcttcgt
      841 ccagcacgtc gttcatcgcc agtgcgaaag cgtcgtgata ggtcaggccg ctttcctggg
      901 cgatcttctg tgccgtctcc agcaatgggt accagaacat ggcggcaaac aggaacgccg
      961 ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagtacc tgctcaataa
     1021 tgcgttccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc gggaacagcg
     1081 gctggaacag atgatattca cacagcagct tataggtttc gtaaccgtag cccgcttgca
     1141 acagtttcag cgattcttca aacagacgtg ccggtgggat atcgttcagt aaggttgcca
     1201 ggcgtgggat cggctcggcg gtttccgggc tgatgcgcat gcccaatttg gcggcaaaac
     1261 gaaccgcgcg gagcatacgt accggatctt cacggtagcg cgtttccggg ttaccaatca
     1321 gacggataac gccgtctttc agatccttca tgccgccaac gtaatcacgg acggtaaaat
     1381 ctgccacgct gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga
     1441 tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc
     1501 ggtcgctgac gttaccttcg tgatgtccac ggaaggtcgc aacttcgata atctccgggc
     1561 caaacatcac atgagccaga cggaaacggc gacccaccag gcggcagtta cggaacagtt
     1621 tgcgcacctg ctctggcgtg gcgttggtgg tcacgtcaaa atctttcggc tttttgccaa
     1681 gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct ttattgagcc
     1741 tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca tgctgctcac
     1801 gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc
     1861 ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc ggtagtgtca
     1921 aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg catttgagaa
     1981 tgttgaattt acaattgccg actcaggcac ggcggtcagc gtccagtttt tgacggctga
     2041 ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgtt tctacctgct gacccagaaa
     2101 atgaagtgcc gcgattaaca ccgggcgcgg atcgcctttc ggcaacgcag gcgcatgatt
     2161 ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggtaaatgga tgtaatctgg
     2221 cactttccag ccaaaaagct ggtacagcga gatttgccgc accgtcggtt cgatcaaatc
     2281 tgccccgcgg acaatttcgc tcacgccctg gaagtgatca tccaccacca ccgccaggtt
     2341 ataggcaaac aaaccatcac ggcgatgaat aataaaatct tcccgtgcca gtttttcgtc
     2401 ggcgtgaata atgcctcgca gcagatcagt aaactgggtg accggatgct gctggcggat
     2461 acgtactgcg gcgttgtctg gtccatgatg caaatcccgg caatgaccgt cgtaaatacc
     2521 gccaatgctt tgaatacgcg cacgcgtgca ggtgcagtaa taacttagtc cttgctcatg
     2581 taaccaggcg agtgcttcgc gataggcgtg gtgacgttgc gattgccaga gaacgtcgcc
     2641 gtcccagtac aggccgtaat gttccagctg gcgcaggata gtttctgcgg caccgggaac
     2701 ttcacgaggc gggtcgatat cttcaatgcg taccagccag cgaccttgcc gggcgcgagc
     2761 ctgcaaatag ctgccaagcg cagcaatcag agagccaaaa tgaagctcgc cggaaggaga
     2821 gggggcgaag cgaccaatat agtgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg
     2881 gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag ccatctgttt
     2941 ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc
     3001 cagacggcga ataccgattt caacaccgca ggattcgcag tagccgaaat cttcgtcttc
     3061 cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat cgcggttacg
     3121 cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg ggaagttggc
     3181 tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt gattacgcca
     3241 tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact cttcgcccgg
     3301 cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg atgttttacg
     3361 gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc catgttcggg
     3421 ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta tctaaacgta
     3481 acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac
     3541 cagttactta ctctactaca atgtaaccgg tagtgatttt ttaagagcca tgccttcggc
     3601 agaaagttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt ctgacaatag
     3661 ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag
     3721 cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgctcatca actcccgaag
     3781 gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa
     3841 cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca acataaagtc
     3901 aatacggctg cgttcggcgc cgtattttac ttcgcttttc agcgagctat agcctgccag
     3961 ctctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt
     4021 gacgcaaata attgcgccgc tctggctttg agttaattcc caggtgtgtg ggtatttccg
     4081 tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac aaccggtcat
     4141 cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc
     4201 taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag aaaattccat
     4261 tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt
     4321 ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgtatacga ccagttaaaa
     4381 cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga
     4441 aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag catattcgcc
     4501 agctggatta agccgcgtgg tggctgtcgc atccctaacc acaccacacg cgaacgcagc
     4561 cattgaccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa
     4621 agagaaagcg ccttctcttt ctctgcgctt acttcaccta aaaatgccag agtcagatgc
     4681 aaattatcgg cggcgaccgg acgtcccgcg tcaggtggga agtgtgtggc gcgccagtgg
     4741 ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagccg ttgcggttca
     4801 gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc gaagaatgtt
     4861 aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttacc tgagttactt
     4921 gctgccctcg atggtgcatc gcaggtgtta ttaagtgcgc cgaccggggc cgggaaatca
     4981 acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat tatcctgctg
     5041 gagccgcgtc gtctggcggc gcgtaacgtt gcgcaacgac tggcggagct gcttaacgaa
     5101 aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc
     5161 cgcctggaag tggttaccga aggcgtgctg acgcggatga tccagcgtga cccggaactg
     5221 agcggtgttg gattggtgat ccttgatgaa tttcatgagc gcagcttgca ggcggatttg
     5281 gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt
     5341 atgtcggcta cgctggacaa cgaccgcttg cagcaaacgc tgccagaagc gcccgtcgtc
     5401 atctcagaag ggcgctcgtt tccggttgaa cgccgttatt tgccgctgcc cgctcatcag
     5461 cgttttgacg aagccgtcgc ggttgctacc gccgaaatgc tgcgtcagga aagtggatca
     5521 ttactgttat ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca actgacttca
     5581 cgcatcggca gtgatgtgtt gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag
     5641 cgaaaagcga tcctcccggc accgcaaggg atgcgtaaag tggtgctggc gaccaatatt
     5701 gctgaaacaa gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc ccaggaaagg
     5761 gtggcgcgtt ttgatccgcg tacagggctt acgcggttga ttactcaacg cgttagtcag
     5821 gcgtcgatga cgcaacgtgc cgggcgtgcc gggcgtctgg agccgggtat ctgcctgcat
     5881 ttaatcgcca aagaacaagc agaacgcgct gccgcacaaa gtgaaccgga gatcttacaa
     5941 agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga tccggcgcag
     6001 atgagctgga tggatcaacc gccaacggtg aatctactgg ccgcgaaacg cctgttacgg
     6061 atgctggggg cgctggacgg tgaacggctt agtgcgcaag ggcaaaaaat ggcaacgctg
     6121 ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga cgaagctgct
     6181 accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa tagtgacctg
     6241 ggcgtggcgt tttcgcgcaa tcaacccgcc tggcagcaac gtagtcagca actgttaaaa
     6301 cgtttaaacg tacgcggcgg tgaggcagac agttcgctta tcgcgccgct acttgccgga
     6361 gcgtatgccg atcgtattgc tcgccgccgt gggcaagagg gacgctatca actggcgaac
     6421 ggcatggggg cgatgctcga tgccgacgat gcgctaagcc gccacgaatg gttgatcgca
     6481 ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc gctgccggtc
     6541 gatattgatg agttagtaca acgctgcccg cagttggtac agcagtctga cactgtggag
     6601 tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaattgg tcagttgatg
     6661 gtaaaagtgc aaccgctggc gaaaccctcg gaagacgagt tgcatcaggc gatgcttaac
     6721 ggcattcgtg ataaaggttt aagcgtgctc aactggacgg cggaagcgga acagctacgc
     6781 ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc ggttgatgat
     6841 gaaagtttgt tggcgacgct ggaaacgtgg ctactgccgc atatgacagg cgtacattcg
     6901 ctacgcggcc tgaaatcact cgatatttat caggcactgc gtggattact tgattgggta
     6961 atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac gggaagccgg
     7021 atcgccattc gttaccatga agataacccg cccgcgctgg cggtgagaat gcaggagatg
     7081 tttggtgagg ccactaatcc gactatcgcc caggggcgcg taccgttggt gctggagttg
     7141 ctttcccctg cccaaaggcc gctgcaaatc acgcgtgatt tgagcgcctt ctggaaagga
     7201 gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccaaacatgt ctggccggac
     7261 gacccggcaa acaccgcacc gacgcgacga acgaaaaagt attcgtgaag accaaagcaa
     7321 atttgagaga tatcttcttc tgtcttgcaa cagaagaaca gaaaatcggg cttttgcgcc
     7381 tgaatattgc ggagaaaaag catggccggg aatgaccgcg agccaattgg acgcaaaggg
     7441 aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga tgacgatgat
     7501 tacgacgatt atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa
     7561 gggcgtaagc ctcgtggcaa acgcggctgg ctatggctgc tgctaaaact ggctatcgtt
     7621 tttgccgtgc tgatcgccat ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt
     7681 gatggcaagg tctggcaatt gcccgcggca gtttatggcc gaatggtcaa ccttgagcca
     7741 gacatgacca tcagcaagaa cgagatggtg aagctgctgg aggcgaccca gtatcgccag
     7801 gtgtcgaaaa tgacccgtcc tggcgagttt accgtacagg ccaacagcat tgagatgatc
     7861 cgccgtccgt ttgatttccc ggacagcaaa gaagggcagg tgcgcgcgcg tctgaccttt
     7921 gatggcgatc atctggcgac gatcgtcaat atggagaaca accgtcagtt cggtttcttc
     7981 cgtcttgatc cgcgcctgat caccatgatt tcttcgccaa acggtgagca gcgtctgttt
     8041 gtgccgcgca gtggtttccc ggatttgctg gtggatactt tgctggcgac agaagaccgt
     8101 catttctacg agcatgatgg gatcagtctc tactcaatcg gacgtgcggt gctggcaaac
     8161 ctgactgccg gacgcacggt gcagggggcg agtacgctga cgcaacagtt ggttaaaaac
     8221 ctgttcctct ccagcgagcg ttcttactgg cgtaaagcga acgaagccta catggcgctg
     8281 atcatggacg cgcgttacag caaagaccgt attcttgagc tgtatatgaa cgaggtgtat
     8341 ctcggtcaga gcggcgacaa cgaaatccgc ggcttcccgc tggcgagctt gtattacttt
     8401 ggtcgcccgg tagaagaatt gagcctcgat cagcaggcgc tgttggtggg tatggtgaaa
     8461 ggggcgtcca tctacaaccc gtggcgtaac ccgaaactgg cgctggagcg acgtaacctg
     8521 gtactacgtt tgctgcaaca gcaacaaatt attgatcaag aactctacga catgctgagt
     8581 gcgcgtccgc tgggtgttca gccgcgcggt ggggtgatct cccctcagcc agcctttatg
     8641 cagctggtgc gtcaggagct gcaggcgaaa ttaggcgata aggtaaaaga tctctccggc
     8701 gtgaagatct tcactacctt tgactcggtg gcccaggacg cggcagaaaa agccgccgtg
     8761 gaaggcattc cggcactgaa aaaacagcgt aagttgagcg atcttgaaac cgcgattgtg
     8821 gttgttgacc gctttagcgg tgaagttcgt gcgatggtcg gtggttctga gccgcagttt
     8881 gcgggctaca accgtgcgat gcaggcgcgt cgttcgattg gttcccttgc aaaaccagcg
     8941 acttatctaa cggccttaag ccagccgaaa atctatcgtt tgaatacgtg gattgccgat
     9001 gcgccaatcg cgctgcgtca gccgaatggc caggtctggt caccgcagaa cgatgatcgt
     9061 cgttatagcg aaagtggcag ggtgatgctg gtagatgcgt tgacccgttc gatgaacgtg
     9121 ccgacggtga atctgggcat ggcgttgggg ctacctgcgg ttacggagac ctggattaaa
     9181 ctgggtgtgc cgaaagatca actgaatcca gttccagcaa tgttgttggg ggcgttgaac
     9241 ttaacgccaa tcgaagtggc gcaggccttc cagactatcg ccagcggtgg taatcgtgct
     9301 ccgctttccg cgctacgttc agtcatcgca gaagacggca aggtgttgta tcagagcttc
     9361 ccgcaggcgg aacgcgctgt tccggcgcag gcggcgtatc tgacactatg gaccatgcag
     9421 caggttgtac aacgcggtac gggtcgtcag cttggggcga aatacccgaa cctgcatctg
     9481 gcagggaaaa cagggactac caacaataac gtagatacct ggtttgcggg cattgatggg
     9541 agcacggtga ccatcacctg ggtcggccgt gataacaacc agccgaccaa actgtatggt
     9601 gccagcgggg caatgtcgat ttatcagcgt tatctggcta accagacgcc aacgccgctg
     9661 aatcttgttc cgccagaaga tattgcagat atgggcgtgg actacgacgg caactttgtt
     9721 tgcagcggtg gtatgcgtgt cttgccggtc tggaccagcg atccgcaatc gctgtgccag
     9781 cagagtgaga tgcagcagca accgtcaggc aatccgtttg atcagtcttc tcagccgcag
     9841 caacagccgc aacagcaacc tgctcagcaa gagcagaaag acagcgacgg tgtagccggt
     9901 tggattaaag atatgtttgg gagtaattaa cgtctaagcg cgatataccg gatggcaagc
     9961 tgccatccgg taaaataaca tcccctccaa agtattaacc ctttcttttc atctgattgt
    10021 ttattgaccc ttcaggaatg tgcagattgc gtaccgcttg cgaacccgcc agcgtttcga
    10081 atattatctc atctttataa taatcattct cgtttacgtt atcattcact ttacaccaga
    10141 gaaataccaa tggcaccttc caaaactgct cagccaaaac actcactgcg taaaatcgca
    10201 gttgtagtag ccacagcggt tagcggcatg tctgtttatg cacaggcagc ggttgaaccg
    10261 aaagaagaca ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc atgggggccg
    10321 gctgcaacta ttgcggcgcg acagtcagct accggcacta aaaccgatac gccgattcaa
    10381 aaagtacctc aatctatttc tgtcgtcacc gctgaagaga tggcgctgca tcagcctaag
    10441 tcggtgaaag aagcgcttag ctatacccct ggcgttgctg tgggaacccg tggcgcatct
    10501 aatacttatg attacctgat catccgcgga tttgcggcag acggccaaag ccagaacaac
    10561 tatctgaatg gcctgaagat gcagggcaac ttctataacg atgcggttat tgacccatat
    10621 atgttggagc gcgctgaaat catgcgtggc ccggtttccg tgctgtatgg gaaaagcagc
    10681 ccaggcggcc tgttgaacat ggtcagcaag cgcccgacca ctgaaccgtt gaaagaagtt
    10741 cagtttaagg ccgggactga cagcctgttc cagaccggtt ttgacttcag cgatgcactg
    10801 gatgatgacg gcgtttattc ttatcgcctg accggtcttg cgcgttctgc caatgcccag
    10861 cagaaagggt cagaagagca gcgttatgct attgcaccgg cgttcacctg gcgtccggac
    10921 gataaaacca atttcacctt cctttcttac ttccagaacg agccggaaac tggttattac
    10981 ggctggctgc cgaaagaggg gaccgttgag ccgctgccaa acggtaagcg tctgccgaca
    11041 gactttaacg aaggggcgaa gaacaacacc tattctcgta acgagaagat gattggttat
    11101 agcttcgacc acgaatttaa cgacaccttt actgtgcgtc agaacctgcg ctttgcgcaa
    11161 aataaagttt cgcaaaagag cgtatatggc tacggtatgt gttcagatcc gctgtatacc
    11221 aaagaccagg aggcactcaa ggcgagtcca tgtttaagta tcccgcagtc tcaatggggc
    11281 catacactga ctcgtcagta cgttatcgat aatgagaagt tagaaaactt ctctgttgat
    11341 actcagttgc aaagcaaatt cgcaaccggt tctgtggatc acactctgct gactggcgtt
    11401 gactttatgc gtatgcgtaa tgacatcgac tcctggttcg gatatgccgg ttcagttgca
    11461 ccatctgata tctataattt agaccgtagt gactttgatt ttggcgctca cccgaatccg
    11521 tctggtccat accgcgttct gctgaaacag aaacaaaccg gcctgtatgt tcaggatcag
    11581 gcgcagtggg ataaagtgct ggttacgctt ggcggtcgct atgactgggc cgatcagtca
    11641 tctttcaacc gtgattacgg taataaatct gaacgtgacg acaaagagtt cacctggcgt
    11701 ggtggtgtaa actacctgtt cgacaacggc gtaacgcctt acttcagcta cagtgaatca
    11761 tttgaacccg cttcacagac tgatgcgaac ggtgatctgt ttgcaccttc gaaaggcaaa
    11821 cagtatgaag tcggcgtgaa atatgtgcca gaagatcgcc caattgtggt gactggcgca
    11881 ctgtatcagc ttaccaaaac taataacctg atggcggatc cgaacggttc actgttctcg
    11941 gttgaaggcg gtgagattcg tgcgcgtggc gtagagctgg aagcgaaagc ggcactgtcg
    12001 gcgagtgtta acgtagtcgg ttcttatact tacaccgatg cggaatacac taccgatact
    12061 acctacaaag gcaatacgcc tgcacaggtg ccaaaacaca tggcttcgct gtgggctgac
    12121 tataccttct ttgacggtcc gctttcaggt ctgacgctgg gcaccggtgg tcgttatact
    12181 ggctccagct atggtgatcc ggctaactcc tttaaagtgg gaagttatac ggtcgtggat
    12241 gcgttagtgc gttatgatct ggcgcgagtc ggcatggcgg gctccaacgt ggcgctgcat
    12301 gtcaacaacc tgtttgatcg tgaatacgtc gccagctgct ttaacactta tggttgcttc
    12361 tggggcgcag aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt tctcttttgg
    12421 ggcacggatt tccgtgcccg tttcacaagt tggctgttat gcaggaatac acgaatcatt
    12481 ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc acgcttttgc
    12541 atccgttgtc gttaaccttt cctgccggga aagtgactgg tctgattggt cacaacggtt
    12601 ctggtaaatc cactctgctc aaaatgcttg gtcgtcacca gccaccgtcg gaaggggaga
    12661 ttcttcttga tgcccaaccg ctggaaagct ggagcagcaa agcgtttgcc cgcaaagtgg
    12721 cttatttacc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa ctggtggcga
    12781 ttggtcgtta cccgtggcat ggcgcactag ggcgctttgg tgcggcagat cgcgaaaagg
    12841 tcgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg gtcgatagcc
    12901 tttctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag gatagccgct
    12961 gtctgttgct cgacgaaccg acttcggcgc tggatatcgc ccaccaggtt gatgtactgg
    13021 cgctggtgca tcgtttaagt caggagcgag gcctgacggt cattgccgtg ctgcacgata
    13081 tcaatatggc ggcgcgctac tgtgattatc tggtcgccct gcgcggcggt gaaatgattg
    13141 ctcagggaac gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt tatggcatcc
    13201 cgatgggtat tctgccgcat ccggcgggtg ctgcacctgt gagttttgtt tattgatgag
    13261 cggcttacct cttatttcgc gccgccgact gttaacggcg atggcgcttt ctccgttgtt
    13321 atggcagatg aataccgccc acgcggcagt tattgatccc aatcgtattg tggcactgga
    13381 gtggttgccg gtggagttac tgctggcgct cggcatcgtg ccttacggcg tggcggacac
    13441 catcaactat cgcctgtggg tcagcgaacc gccattgccg gactcagtga ttgacgttgg
    13501 tttgcgtaca gaacccaacc ttgaactgct gaccgaaatg aaaccgtcgt ttatggtctg
    13561 gtctgcagga tatggccctt caccagaaat gctggcacgc attgcgccgg ggcgcggatt
    13621 taacttcagc gatggcaaac agccgctggc gatggcgcgt aaatcactga cggaaatggc
    13681 agatttactt aacctgcaaa gcgcagcgga aacgcattta acgcactatg aagactttat
    13741 ccgcagcatg aaaccccgct ttgtgaagcg tggtgcacgc ccgttattgc tgacgacgct
    13801 tatcgatccg cgccatatgt tggtcttcgg tccaaacagc ttgttccagg aaattctcga
    13861 tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca gtaccgccgt
    13921 cagtatcgat cgtctggcgg cgtataaaga cgttgatgtg ctctgttttg atcacgacaa
    13981 cagcaaagac atgaatgcgc taatggcaac cccgctgtgg caggccatgc cgtttgtccg
    14041 cgccggacgc tttcagcgcg ttccggcggt ctggttctat ggcgcgacgc tttcggcaat
    14101 gcactttgtg cgtattctgg ataacgccat cggaggtaaa gcgtgagtaa acgaattgcg
    14161 cttttcccgg cgttattgct ggcgctgctg gtggttgccg catcggcgct cacctggatg
    14221 aactcctcgc aggcgctgcc gcgcagccag tgggcgcagg ccgcctggtc gccggatatt
    14281 gacgtcatcg agcagatgat ttttcactac agcttgctgc cgcgtctggc gatttcgctg
    14341 ctggtgggcg cgggcctggg actggtgggc gtgctgtttc agcaagtgct gcgtaacccg
    14401 ctggcggaac cgacgacgtt gggcgttgct acaggcgcgc aactggggat taccgtcacg
    14461 acgctctggg cgatccccgg cgcgatggcg agccagtttg ctgcgctggc gggcgcttgc
    14521 gttgttggct taatcgtctt tggcgtcgcg tgggggaaac ggctttcgcc ggtaacgctg
    14581 atcctcgcgg ggctggtggt gagcctttat tgtggggcaa tcaatcagtt actggttatc
    14641 ttccatcatg accaactgca aagcatgttc ctgtggagca ccggaacact gacgcaaacc
    14701 gactggggcg gcgttgagcg tttatggccg caactgctgg gcggcgtgat gctgacgtta
    14761 ttgctacttc gcccgttaac tctgatgggg cttgatgatg gcgtggcgcg caatctcggg
    14821 ctggccttgt cgctcgctcg tctggcggcg ttgtcgctgg cgattgtcat cagtgcgctg
    14881 ctggtgaacg cggtggggat tatcggcttt atcgggttgt tcgcgccact gctggcgaaa
    14941 atgctggggg cgcggcgttt gttgccacgg ttgatgctgg catcgctgat tggtgcgtta
    15001 attctgtggc tttctgatca aatcatcctc tggctgactc gcgtgtggat ggaagtgtcc
    15061 accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggttgct gccgcgttta
    15121 cgcagcatta gcgcgccgga tatgaaggtg aacgatcgtg tcgcgactga acgccaacac
    15181 gtgctggcgt ttgccctcgc gggcggcgtg ctgctgttga tggctgtggt ggtggcgctg
    15241 tcgttgggtc gtgatgcgca cggctggacg tgggcgagcg gggcgttgct cgaggatttg
    15301 atgccctggc gctggccgcg aattatggct gcgctgtttg cgggcgtcat gctggcggtg
    15361 gcgggctgta ttattcagcg gctgaccgga aacccgatgg caagcccgga agtgctgggg
    15421 attagctccg gcgcggcgtt tggcgtggtg ttaatgctgt ttctggtgcc gggtaatgcc
    15481 tttggctggc tgttacccgc aggcagtctc ggggcggcgg tgacgctgtt gatcattatg
    15541 atcgccgccg gacgtggtgg attttcccca caccgcatgt tactggcggg gatggcgtta
    15601 agtaccgcgt tcaccatgct tttgatgatg ttgcaggcaa gtggtgaccc gcgaatggcg
    15661 caagttctga cctggatttc cggttcgacc tacaacgcga ccgatgcgca ggtctggcgt
    15721 accggaattg tgatggtgat tttgctggcg attaccccgc tgtgccgccg ctggctgacc
    15781 attttaccgc tgggtggtga taccgcacga gccgtgggga tggcgctgac gccgacgcga
    15841 attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacaatgac cattggaccg
    15901 ttgagttttg ttggtttaat ggcaccgcat attgcgcgga tgatgggctt tcgacggacg
    15961 atgccacaca tcgtaatttc ggcgctggtg ggtggtttac tgctggtgtt cgctgactgg
    16021 tgtgggcgga tggtgctgtt tccattccag atccccgcgg ggctgctgtc gacctttatc
    16081 ggcgcgccat attttatcta tttgttgaga aagcagagcc gttaattgaa tgcctcgccc
    16141 ggtgggcgag gcaaaatcat cacaacttcg caaacacccg acgtgcagca tcgatggtgt
    16201 tattgatatc ttccatgctg tgcgccacag acataaagcc cgcttcaaac gctgacggtg
    16261 ccagataaac accttcgtcc agcatcatat ggaagaaacg cttaaaacgt tccacgtcac
    16321 aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaaa ataccgaaca
    16381 tgccgccaac gtggtttacc accagcggaa ttccggcttc ttctgccgct tcccgcagac
    16441 cttctgccag acgtgttgtc agctcatcca gcgtctcgtg aacgcccggc tgtgcgactt
    16501 cattcagaca ggcgaaacca gcagccatcg caatcgggtt accggaaagc gtacccgcct
    16561 gatagaccgg accggtcggg gccagcgcat ccattacatc acgacgacca ccgaatgcgc
    16621 ctaccggcat tccaccgccg atgattttgc cgaggcaggt gagatccggt tccacaccgt
    16681 aataatcctg tgcgccagct agcgctacgc ggaaaccggt catcacttca tcgatgatca
    16741 gcaacgcgcc aaattcgtcg cacagcgcgc gcagacctgg caggaactct ggcagcggtg
    16801 gaacgcagtt catattgcct gccaccggct cgacgataat acaggcaatc tcttgcgggt
    16861 attgctcaaa cgcggcgcgt acagaggcca gatcgttata agtacaggtt aaggtatgtt
    16921 tggcgaaatc tgccggaacg cccggcgaat ttggctggcc taacgtgagt gcgccagaac
    16981 cggctttcac cagcaggcag tcagcgtgac cgtggtaaca accttcaaat ttaataattt
    17041 tgtcgcgacc ggtaaaacca cgggccaggc ggatggcgct catcgtcgct tcagtgccgg
    17101 agttcaccat gcgcaccata tccatggtcg ggaccagttc ggtcaccagt tgcgccattt
    17161 tcacttccat ttcggttggt gcaccaaagc ttaaaccacg ctcggcggct tcaatcacgg
    17221 cattgcggat cgccggatgg ttatggccca gcaccatcgg cccccaggaa ccgacataat
    17281 cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt tcgataaaca
    17341 gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacgccg ccagggatca
    17401 gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc tgattcgtag
    17461 aaaaagtgaa tggctgctat tctatgttat tcataacaag ttaaatactc gtcaaacatc
    17521 aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg tgcatttgaa
    17581 acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt catcttattt
    17641 gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga aacaccgcag
    17701 gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga taaaaccccg
    17761 ttagccattt tgtttatggc ggcagtcgtc ggcacgcttg tcgggctggc tgcggttgct
    17821 tttgacaaag gtgtcgcctg gttgcagaac caacgtatgg gggcactggt acatactgct
    17881 gataattatc cgcttctgtt aaccgtcgct tttctctgtt cggcggtgct ggcgatgttc
    17941 ggctactttt tggtgcgcaa atacgcgccc gaagcagggg gatcggggat cccggaaatt
    18001 gaaggggcgc tggaagatca acgtcccgtt cgctggtggc gtgtgttgcc ggtgaaattc
    18061 tttggcgggc tggggacact cggcggaggc atggtgctgg ggcgcgaagg gccaaccgtg
    18121 cagatcggcg gtaatatcgg gcggatggta ctcgatgttt tccgcctgaa aggtgacgaa
    18181 gctcgtcata cgctactggc aaccggtgct gcggcggggc tggctgcggc ctttaacgcg
    18241 ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg ctatacgtta
    18301 atttcgatta aagcggtatt tattggcgtc attatgtcga ccattatgta ccggattttt
    18361 aatcatgaag ttgcgttgat tgacgtcggt aaactttctg atgcgccgct taatacgctg
    18421 tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt taataaatgg
    18481 gtgctgggga tgcaggattt gctgcaccgt gtgcacggtg gcaatattac caaatgggta
    18541 ctgatgggtg gtgcgattgg cggtctgtgt ggattgctgg ggtttgtggc accagcaact
    18601 tcgggcggcg gttttaacct gattcctatc gctaccgcgg ggaatttcag catgggaatg
    18661 ctggtgttta tcttcgtcgc acgggtcatt accaccttac tctgcttctc ttccggcgcg
    18721 ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac cgctttcgga
    18781 atggttgccg ttgagttgtt tccgcaatat caccttgagg cggggacgtt tgctattgcc
    18841 ggaatggggg cgttactggc ggcatctatt cgcgcgccgt taacggggat cattctggtt
    18901 ctggaaatga ccgataacta ccagctcatt ttgccaatga ttattaccgg tcttggcgca
    18961 acactattag cacaatttac cggtgggaaa ccgctatact cggcgattct tgcgcgcacg
    19021 ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc ccgcgagaat
    19081 acttgaacga aataccaggg tattagataa tggcgattat tattgggtta gaatttgccc
    19141 aattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc gctggagttt
    19201 accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa cccgaatctg
    19261 aaattacgcg tgtatatcac cggtggcggt tgcagcggct tccagtatgg tttcaccttt
    19321 gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg cctggtggtt
    19381 gatccgatga gcctgcaata tctggtcggc ggttccgttg actataccga aggtctggaa
    19441 ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg cggttcttcc
    19501 tttagtatct aatctgctgt tcatgtgccg gataaactat ccggcacatc ttctctcttc
    19561 aacgaccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga atagccgcca
    19621 gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt ggtacggaaa
    19681 atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt gcgtagattt
    19741 ctgtacgtaa aatcatgggg atttcgcggg ccagtacatc acgaatgatc ccgccgccaa
    19801 cgccagtaat gacgcccata caaaccgcga ttaacggacc ggcttccgca ttaaaggctt
    19861 tattcacgcc aatgccgaca aacaccgcca gaccaacggc gtccagcacc ggcaacatcc
    19921 attttggtaa gcgtcttggc tggcgcacca gcatgatggt cagcatgctg gtgaccattg
    19981 caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc agcgccatgt
    20041 cgcgaattgt tccgccgcct actgcggtaa ccacgcccaa taccagaaca ccaaaagggt
    20101 ccatacgcaa ttttccggct aacaaaacgc cagagatagc aaataccgct gtgccgacta
    20161 tatccagcca atagacgagc attgttcaat ccccactgag cacctgaaaa ggtcaggcac
    20221 taatctacct gtgaaagcgc attacagagc tgttgtgcag cgaggataat acgtgggctt
    20281 gcacgttcaa accagtcact cgtgagagga ataacgggaa ttttgagctg ttcaccccag
    20341 tattgtttga ttttaggaat ttggtccggt ccgcctgtaa tgacaatcgc ctgtggcgag
    20401 cgtgctaaca cctgttcgcg gctaacttgc ggccagggaa cccggctgtc tttaaagatg
    20461 ttttctccgc cacaaacttc gagtacctgg ttctgaatcg actcttttcc actggtaaat
    20521 ggcggattaa tgccgaattg cagaaaaaca cgttttttag gtttatcagc atattgcgct
    20581 ttcaattgcg cgtactgatc cagcagggat tgcgcggctt gttcggcctt atccggttgc
    20641 ggactccagg gggccagttg acgtaacgca ttggcaattt gttcaatgct tgtcgcatcg
    20701 acccacatca cttttattcc cagcgaagcc aactggtcaa cctgccgctc ggcattacct
    20761 ccacgccagg caatcaccag atcgggtttc agcgcgacaa tgcgttccag attcatcccc
    20821 tgccaggtgg aaacctgctc aatcttttgc gcttgtggag gatagtcgga atagctgctg
    20881 accccaaccg gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg agaaagcgtg
    20941 atgacgcgcg gcgcggcatt gagccacagc ggcgcgagaa aagacagggc gaccagcgcc
    21001 ctgaacagtg acttagccat gtgccagttt ctgcaccagt gactcaacca tcaggctgga
    21061 ctgtttagcg gcaacagcca ggaactcatc gaagctaaga tgagactgtt gatcggccac
    21121 gtcggagatg gcgcgtacta cgacaaacgg gacgttgaaa ttgtggcaga catgggcgat
    21181 tgccgtcgct tccatctcta cagcaatggc ctgtgggaag ttgtggcgga ttttcgccag
    21241 accaacagaa ccgttgatga aagcgtcgcc gctaacaatc aggccacgta cagcgttaag
    21301 attcagttcg gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag ctttgaagcc
    21361 tgccgggcag cctggtaact gaccgtattc ataaccaaat gccgtgacat ccgcgtcgtg
    21421 ataacgcgct tcgtccgaga caacgatatc gcccactttc agcgttggtg ccaggccacc
    21481 ggcagaaccg gtgttaataa tcacatctgg cttgcagtgc tccaacagca aagtggcacc
    21541 cagcgccgca gcgactttac cgatgcccga tttcagaagc gcaacctcgg ttccattcag
    21601 ttggccggta tagatttcgc aaccgcccag actgatagtt tgacggtttt cgattttgtc
    21661 acgcagcagc gtaacttctt cttccattgc accaatgatg ccgattttca tagatttact
    21721 cgcgataagc ccgatttgaa ggcatagttt accatgcgct tacggggaag cgtatttctc
    21781 acgcgggaga ggacatggca cagattgatt tccgaaaaaa aataaactgg catcgtcgtt
    21841 accgttcacc gcagggcgtt aaaaccgaac atgagattct gcggatcttc gagagcgatc
    21901 gcgggcgtat catcaactct ccggcaattc gtcgtctgca acaaaagacc caggtttttc
    21961 cactggagcg caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa gtccagcagg
    22021 tggggcgcta catcgctaaa gaaattttaa gccgtttgaa agagcttaaa ttactggagg
    22081 cgtatggtct cgatgaactg accgggccct ttgaaagcat tgttgagatg tcatgcctga
    22141 tgcacgatat cggcaatccg ccgtttggtc attttggcga agcggcgata aatgactggt
    22201 ttcgccagcg tttgtacccg gaagatgccg aaagccagcc tctgactgac gatcgctgca
    22261 gcgtggcggc actacgttta cgggacgggg aagaaccgct taacgcgctg cggcgcaaga
    22321 ttcgtcagga cttatgtcat tttgaaggta acgcacaggg tattcgtttg gtgcatacat
    22381 tgatgcggat gaatctcacc tgggcacagg ttggcggtat tttaaaatat acccgtccgg
    22441 cgtggtggcg tggcgaaacg cctgagacac atcactattt aatgaaaaag ccgggctatt
    22501 atctttccga agaagcctat attgcccggt tgcgtaaaga acttaatttg gcgctttaca
    22561 gtcgttttcc gttaacgtgg attatggaag ctgccgacga catctcctat tgtgtggcag
    22621 accttgaaga tgcggtagag aaaaggatat ttaccgttga gcagctttat catcatttgc
    22681 acgaggcgtg gggccagcat gagaaaggtt cgctcttttc gctggtggtt gaaaatgcct
    22741 gggaaaaatc acgctcaaat agtttaagcc gcagtacgga agatcagttt tttatgtatt
    22801 tacgggtaaa caccctaaat aaactggtac cctatgcggc acaacgattt attgataatc
    22861 tgcctgcgat tttcgccgga acgtttaatc atgcattatt ggaagatgcc agcgaatgca
    22921 gcgatcttct taagctatat aaaaatgtcg ctgtaaaaca tgtgtttagc catccagatg
    22981 tcgagcagct tgaattgcag ggctatcggg tcattagcgg attattagag atttatcgtc
    23041 ctttattaaa cctgccgtta tcagacttta ctgaactggt agaaaaagaa cgggtgaaac
    23101 gtttccctat tgaaacgcgc ttattccaca aactctcgac gcgccatcgg ctggcctatg
    23161 tcgaggctgt cagtaaatta ccgtcagatt ctcctgagtt tccgctatgg gaatattatt
    23221 accgttgccg cctgctgcag gattatatca gcggtatgac cgacctctat gcgtgggatg
    23281 aataccgacg tctgatggcc gtagaacaat aaccaggctt ttgtaaagac gaacaataaa
    23341 tttttacctt ttgcagaaac tttagttcgg aacttcgcgc tataaaacga atctgaagaa
    23401 cacagcaatt ttgcgttatc tgttaatcga gactgaaata catgaaaaaa accacattag
    23461 cactgagtgc actggctctg agtttaggtt tggcgttatc tccgctctct gcaacggcgg
    23521 ctgagacttc ttcagcaacg acagcccagc agatgccaag ccttgcaccg atgctcgaaa
    23581 aggtgatgcc ttcagtggtc agcattaacg tagaaggtag cacaaccgtt aatacgccgc
    23641 gtatgccgcg taatttccag cagttcttcg gtgatgattc tccgttctgt caggagggtt
    23701 ctccgttcca gagctctccg ttctgccagg ggggcctggg cggtaatggc ggcggccagc
    23761 aacagaaatt catggcgctg ggttccggcg ttatcattga tgccgataaa ggctatgtcg
    23821 tcaccaacaa ccacgttgtt gataacgcga cggtgattaa agtccaactg agcgatggtc
    23881 gtaagttcga cgcgaagatg gttggcaaag atccgcgctc tgatatcgcg ctgatccaga
    23941 tccagaaccc gaaaaacctg accgcaatta agatggcgga ttctgatgcg ctgcgcgtgg
    24001 gtgattacac cgtagcaatt ggtaacccgt tcggcctggg cgagacagta acttccggga
    24061 ttgtctctgc gctgggacgt agcggcctga acgctgaaaa ctacgaaaac ttcatccaga
    24121 ccgatgcagc gatcaaccgg ggtaactcag gtggtgcgtt ggttaacctg aacggcgaac
    24181 tgatcggtat caacaccgcg atcctcgcac cggacggcgg aaacatcggt atcggttttg
    24241 ctatcccgag caacatggtg aaaaacctga cctcgcagat ggtggaatac ggccaggtga
    24301 aacgtggtga gttgggtatt atgggcactg agctgaactc cgatctggcg aaagcgatga
    24361 aagttgacgc ccagcgcggt gctttcgtaa gccaggttct gccgaattct tccgccgcga
    24421 aagcgggcat taaagcgggt gatgtgatca cctcactgaa cggtaagcca atcagcagct
    24481 ttgccgcact gcgtgctcag gtgggcacta tgccggtagg tagcaaactg accctgggct
    24541 tactgcgcga cgggaagcag gttaacgtga acctggaact tcagcagagc agccagaatc
    24601 aggttgattc cagcaccatc ttcaacggca ttgaaggcgc tgagatgagc aacaaaggta
    24661 aagatcaggg cgtggtggtg aacaacgtga aaacgggcac tccggctgcg cagatcggcc
    24721 tgaagaaagg tgatgtgatt attggcgcga accagcaggc agtgaaaaac atcgctgaac
    24781 tgcgtaaagt tctcgacagc aaaccgtctg tgctggcact gaacattcag cgcggcgaca
    24841 gcaccatcta cctgttaatg cagtaatctc cctcaacccc ttcctgaaaa cgggaagggg
    24901 ttctccttac aatctgtgaa cttcaccaca actccataca tcttcatcat cctttgggca
    24961 tttgcacaat gccgtacgtt acgtacttcc ttatgctaag ccgtgcataa tggaggactt
    25021 atggctggct ggcatcttga taccaaaatg gcgcaggata tcgtggcacg taccatgcgc
    25081 atcatcgata ccaatatcaa cgtaatggat gcccgcgggc gaattatcgg cagcggcgat
    25141 cgtgagcgta ttggtgaatt gcacgaagga gcattgctgg tactttcaca aggacgagtc
    25201 gtcgatatcg atgacgcggt ggcacgtcat ctgcacggtg tgcggcaggg gattaatcta
    25261 ccgttacggc tggaaggtga aattgtcggc gtaattggcc tgacaggtga accagagaat
    25321 ctgcgtaaat atggcgaact ggtctgcatg acggctgaaa tgatgctgga acagtcgcgg
    25381 ttgatgcacc tgttggcgca ggatagccgt ttgcgggaag aactggtgat gaacctgatt
    25441 caggctgagg agaatactcc cgcacttact gaatgggcgc aacggctggg gatcgatctc
    25501 aatcaaccgc gagtggtggc tattgttgag gtcgacagcg gtcagcttgg cgtggacagc
    25561 gcaatggcgg agttacagca actgcaaaat gcgctgacta cgcccgagcg taataatctg
    25621 gtggcgattg tttcgctgac cgaaatggtg gtgttgaaac cggcgttgaa ctcttttggg
    25681 cgctgggatg cagaagatca tcgtaagcga gttgaacagc tgattacccg catgaaagag
    25741 tacggccagc tgcgttttcg cgtttcgctg ggcaactatt ttaccggacc gggcagtatt
    25801 gcccgctctt atcgtacggc gaaaacgacg atggtggtgg ggaaacagcg gatgccagaa
    25861 agtcgctgct atttttatca ggatctgatg ttacctgtgt tactcgacag tttgcgtggc
    25921 gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc tgaaagcgat ggacaataac
    25981 ggcttgctgc ggcgaacgct ggcggcgtgg tttcgccaca atgtgcaacc gctggcaacg
    26041 tcaaaggcgt tgtttattca tcgtaatact ctggaatatc ggcttaatcg tatatcggaa
    26101 ctgaccgggc ttgatttggg caattttgat gacaggttgc tgctgtatgt ggcgttacaa
    26161 ctggatgaag agcggtaggt cgattacaaa attacagccg ggtgagactt tacccggccc
    26221 acagaatggc attatttacg cgtcagtttt tccagatcgg cttcaatctc gctgatctta
    26281 ttggttacta ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact
    26341 tcgctgcggt cacgctggca gatttgatca agctcatcaa tgatataccg tagattcggg
    26401 ctaatttcct ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc
    26461 tgacgcgggt atttaaactt cacgctcttg gcgaaaaact cgcctttgtc tttctggaaa
    26521 tagattttca gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca
    26581 ggattggtaa tacccagact tttcagattg tcgtacatag tgttacctca aaatgagtca
    26641 gtaaattgtg cttattttag catttggcct ggcccgcccg gctcgactgt gatcgcagaa
    26701 agcctttttc ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg
    26761 tggtgatatt ccatgggatg gattagcaca tttatcttca ttttgcgaat tacatgacag
    26821 tccgaaacaa gatgaaaaag tatttaaaaa aaagggggaa aatgagtagg acgaattatt
    26881 ctgagccaga aagcaatcgg gcagcgtcgt gctgcccgtg tacatacttt tagtcgatgg
    26941 tacgcagcag ttcgttaata ccaactttac cgcgagtttt cgcgtcaact ttcttaacga
    27001 taactgcaca gtagaggctg tatttgccat ctttcgacgg cagattacct gaaacaacca
    27061 cagaccccgc cggaacgcga ccgtagtgga tttcgccggt ttcacggtca taaatacggg
    27121 tgctctgacc aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt
    27181 caaccacttc agagcgtgcg ccgatgaagc agttatcttc aataatggtc gggttagcct
    27241 gcagcggctc cagcacgccg ccgataccca cgccaccgga aaggtggacg tttttaccaa
    27301 tctgcgcaca ggaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg
    27361 cgccgatgtt gacgtaagac ggcatcagca cggtgttacg ggcaatgaac gcgccctgac
    27421 gcactgccgc tggtggcacg acgcggaagc cttctttctg gaaacgtgct tcatcgtagt
    27481 tagcgaattt catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat
    27541 tatcattaat acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact
    27601 gaccgtcaat tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat
    27661 ttaccgcttc gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa
    27721 aagcggtttc aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa
    27781 cacatcaccc tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct
    27841 gcaactcgtt attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc
    27901 gctcgccaat ggttgtaatt cgggcgccat gaagcgaaat tcccagatcg gcaaaaattt
    27961 tcccgactcg cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc
    28021 ggtcggtatg ggtcggcaaa aacgtcactt cggtttcaac agtaaaatgg cgcaatttgg
    28081 cgggttggcg acggggctgc ggtggctgcc agctactttg tatcagtact tgctccagac
    28141 caaaccgaat aacctcatga cgatctgcgg atagcgggct accatcgggt tccagcacta
    28201 taaaggtatc catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta
    28261 aattgcggcg gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc
    28321 agataaaaat ctcggtgcct ccacgcgtag cctgcgggct aagcagtacc agcggtttgc
    28381 ttaaatcatg ctgtaataaa tggcgtgcgt gccaggccag ttgatttggg ctatggcgga
    28441 caaaatagtt agcgcgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat
    28501 ccatgcgcag tagtgccagc gcctggagtt ggtgatggcg aacccgttcg cgcatatccg
    28561 gcgtgttttg cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca
    28621 gactttgctt ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca
    28681 ggcataccag atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga
    28741 cttccgggtc ctgaatatcg cggcgctgag cggtcaccga catcagcaag tgctggcgaa
    28801 ccagccatgc gaccagctgt gtttcgcgtg agttcagccc gtggagttcg gcaaaatgca
    28861 ctacatcctg agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa
    28921 acagcgcggc gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca
    28981 acggatggcg ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga
    29041 tagtatgttc atccaccgtg taggcatgga acaggtcaaa ctgcatctgc ccgacgatat
    29101 gcgaccattg cggcatatac gcgccgagca cgctatggcg atgcattggc aatagcccgc
    29161 gccgcaccgc tccggggtga cgcagaatgc tcaaaaacag ttttcgcgct tccggaatat
    29221 tacacagagg ttgttgctgg tggcgacggg cgtggcgtaa ctggcgtagt gtggtggagt
    29281 aaatgccggt gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg
    29341 gctggcgcat aaatagtgtt tcatcacgca ggtcgatcag cgttccgcgt agctgaaact
    29401 catcgtcgat tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca
    29461 gttgcagcag catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca
    29521 tcatccgctc gaccggttcg ttaccttcac cactgtaatt caaacgctgg gcgacgctaa
    29581 gctggcgatc gaataacagg cgattatcgt aacgactgac gaccagatgc agggcaaagc
    29641 gaatgcgcca caatatatgc aggcattcgt ttaattccgc tcgctccgca gaggttaaga
    29701 agccaaaccc taccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt
    29761 gcagagtgtg gatatcgcgc aagccgccgg ggctactttt gatgtctggt tcaagattgt
    29821 agctggtgcc gtggtaacgc tgatggcgct ggttctgttc ttcaactttc gccgcgtaga
    29881 acttgtcgga aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaacagcg
    29941 caacatcgcc aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata
    30001 acccttccag catgcactct tcaagcgtgc gcacgctatg acctacttcc agctttacat
    30061 cccagagcag cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac
    30121 ggcttaaaat cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac
    30181 cgacggcgac caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt
    30241 gaaggagctg gtcgataaac tcggtacgcg cctcaatcaa ctgttctgcg gagataccat
    30301 tgtcaaaggc atcacccagc caacgctgga aagtatcgat atgggctttt atcccaccga
    30361 cggttaattc atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtgggga
    30421 gagcggtgtt tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa
    30481 ttattgcatt aaaaaagccg gcattcgccg gcttcatttt attcgtcgtg cgagattatc
    30541 gccgggatgg tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc agtcaccaca
    30601 atagtatgct catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct
    30661 ttcatggtgc ggatctcttt tttacccgcg ttgaccattg gctcgatggt gaacgtcatc
    30721 cccggtttca gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttcg
    30781 tggaagccgc gaccaatacc gtgtccacaa tattcacgaa cgacggagaa gccttctgct
    30841 tcgacaaatt tctgaattgc cgcaccgatt tcgcgcagat taatgccagg ttttaccatg
    30901 cgtagcgcca ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc
    30961 ggcttaccga cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg
    31021 acatcaatgt taacgatatc gccatctttc agcagcttgg cgtcgtccgg gataccgtgg
    31081 cacaccactt cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag
    31141 gcagaaaccg cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg
    31201 gtgctgacgc ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg
    31261 ccagcgacgc gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct
    31321 gtccatcagc gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc
    31381 cgggcaaatt gagaatcatt ctgaatttcg ccaaacgtgc cactggaggt tttctataat
    31441 agaaaattca acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat
    31501 ttgtggtata aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc
    31561 gacaatctca ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg
    31621 gggtcggtaa tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc
    31681 atggcaactg tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc
    31741 cgttactgga acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc
    31801 atcaaccttg agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt
    31861 gcttctcgca aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg
    31921 aaagacgctg ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg
    31981 ctgactaact ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag
    32041 tctcaggacg gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag
    32101 ctggagaaac tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct
    32161 ctgtttgtaa tcgatgctga ccacgaacac attgctatca aagaggcaaa caacctgggt
    32221 attccggtat ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc
    32281 ccgggtaacg acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc
    32341 gtacgtgaag gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct
    32401 gagtaataag gcacgctcgt acgagccctt atagaccagg tagtacacgt ttggttaggg
    32461 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    32521 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    32581 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    32641 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    32701 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    32761 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    32821 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagta ctgaaagcac
    32881 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc
    32941 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggtg
    33001 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33061 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    33121 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    33181 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    33241 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    33301 ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    33361 cagaagttgc tgcgatgtcc aagcagtctt aattatcgaa aaggagccgc ctgagggcgg
    33421 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    33481 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    33541 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    33601 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgcatgg ctcaggaaat
    33661 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    33721 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    33781 gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    33841 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    33901 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    33961 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34021 gctgaaagca accaaagttg acggcgtgtt tactgctgat ccggcgaaag atccaaccgc
    34081 aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga
    34141 cctggcggcc ttcacgcttg ctcgtgacca taaattaccg attcgtgttt tcaacatgaa
    34201 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    34261 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggatctta
    34321 cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt gactgtctgg
    34381 tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag aaaagatgct
    34441 gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg
    34501 ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg cacgccgacg
    34561 ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa aatcaacgtg
    34621 tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga tcttggcctg
    34681 aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac ggaagaacgt
    34741 cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt
    34801 aatgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa agagatcagc
    34861 gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc tgcaatcaag
    34921 aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg atttcttgaa
    34981 cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt gctttttatt
    35041 ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga ccggctcgat
    35101 tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg tagttgcgct
    35161 ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct ctccccgcta
    35221 tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa atgatgctac agcaacaggg
    35281 gagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggctg cgcttgagga
    35341 tgttgatcag gtgatggcag ccattgttgg cgctgctggg ctgttaccta cgcttgctgc
    35401 gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta cctgcggacg
    35461 tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg atagcgaaca
    35521 taacgctatt tttcagagtt taccgcaacc tatccagcat aatctgggat acgctgacct
    35581 tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt tccgtgagac
    35641 gccattgcgc gatttggcaa caatgacgcc cgatcaagcc tgccgtcatc cgaactggtc
    35701 gatgggacgt aaaatttccg tcgattcggc taccatgatg aacaaaggtc tggaatacat
    35761 tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga ttcacccgca
    35821 gtcagtgatt cactcaatgg tgcgctacca ggacggcagt gttctggcgc agctgggtga
    35881 accggatatg cgtacgccaa ttgctcacac catggcatgg ccgaatcgcg tgaactctgg
    35941 cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac cggattatga
    36001 tcgttatcca tgtctgaaac tggcgatgga ggcgtttgaa caaggccagg cagcgacgac
    36061 agcattgaat gccgcaaatg aaatcaccgt ggctgctttt cttgcgcaac aaatccgctt
    36121 tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc gcgaaccaca
    36181 atgtgtggat gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt
    36241 gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt tgttagcgta
    36301 gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta taaggtcagg
    36361 tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg agtaccgtaa
    36421 accctgtcag ggaataagaa acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa
    36481 ttgccagcgc atggctgtcg tcatgttgcg atcattatgg acggcaatgg tcgctgggca
    36541 aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc cgtccgccgg
    36601 gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc ctttagtagt
    36661 gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg
    36721 ctagatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat tattggcgat
    36781 accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc gctaacagcc
    36841 gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg ggatatagtc
    36901 cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc agatcagata
    36961 gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt agatttagta
    37021 attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat tgcctatgcc
    37081 gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt tgaaggggcg
    37141 ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca
    37201 gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt gttaataccc
    37261 gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccattgt aacgctggtg
    37321 gtctgtatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac tcgttcgcag
    37381 cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt tctgttgccg
    37441 gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc ttcgctgggt
    37501 tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc aatctggcgt
    37561 aactctaaaa cattgcgcat tatttttggc gtgctaacca ttgttccctt cttctggggc
    37621 atgctggcgt tacgggcctg gcactacgac gagaatcatt acagtggcgc aatatggctg
    37681 ctctatgtca tgatcctggt atggggcgct gactctggcg catatatgtt tggcaaattg
    37741 tttggtaaac ataaactagc accgaaggtt tctccgggta aaacctggca aggctttatc
    37801 ggtggactag tgaccgcagc ggtaatctca tggggttatg gcatgtgggt gaatctcgac
    37861 gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc agtgctcggc
    37921 gatctgaccg aaagtatgtt taagcgtgaa gcaggaatta aggacagcgg tcatttaatt
    37981 ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt accggtcttt
    38041 gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg ctgagttttc
    38101 tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg catgaatttg
    38161 gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca atagggtttg
    38221 gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc gccctgatcc
    38281 cgttgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt ccggaactcc
    38341 gccaccatgc cttcaataat aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc
    38401 cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt attggtgtgc
    38461 ctggcgtacg tccggtggtt ggtgaaatag cagccaattc gatagctgcg gaagcacaaa
    38521 ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat tgggatgccg
    38581 tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattacg gtagcgccat
    38641 ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg tttgagcctg
    38701 ataaagaaga tccggtatct tcgttgggga ttcgtcctcg tgggccgcaa attgaacctg
    38761 tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca
    38821 ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc
    38881 gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt cccttgtctt
    38941 tgacattaat cccggagagt aaaccgggta agggtaaagc gattggtttt gtcggtattg
    39001 agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat gggccgttca
    39061 acgctatcgt cgaagccacg gacaaaacgt ggcagttgat gaagctgacg gtcagtatgc
    39121 tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg atctctatcg
    39181 ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg tttcttgcgc
    39241 ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt gacggggggc
    39301 atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag cgggttcaag
    39361 acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt gcacttttca
    39421 atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga tggcgatgaa
    39481 aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg ctgaagggtt
    39541 cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct
    39601 cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta ataccattcg
    39661 cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg gtgataccct
    39721 tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg gtaacaaatc
    39781 ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc
    39841 cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct actacagcgt
    39901 cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga
    39961 cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta acattgttgg
    40021 taaccatgct ttcaccaccg atgaactgat ctctcatttc caactgcgtg acgaagtgcc
    40081 gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg gcgaccttga
    40141 aaccctgcgc agttactatc tggatcgcgg ttatgcccgt ttcaacatcg attctaccca
    40201 ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga
    40261 tcagtacaag ctttctggcg ttgaagttag cggcaatctt gccgggcact ccgctgaaat
    40321 tgagcaactg actaagatcg agccgggcga gctgtataac ggcaccaaag tgaccaaaat
    40381 ggaagatgac attaaaaagc ttcttggtcg ctatggttat gcctatccgc gcgtacagtc
    40441 gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg
    40501 taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga aagatgccgt
    40561 cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg gggagcgatc tggtcgatca
    40621 gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg atacccaacg
    40681 tgttccgggt agcccggacc aggttgatgt agtctacaag gtgaaagaac gtaacaccgg
    40741 tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc aggctggtgt
    40801 gcaacaggat aactggttag gtacgggtta tgctgttggt atcaacggga ccaaaaacga
    40861 ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag atggcgtaag
    40921 cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc tgtccgacta
    40981 taccaacaag agttatggta cagatgtgac gttgggcttc ccgatcaacg aatataactc
    41041 gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc ctcaggttgc
    41101 gatgtggcgc tatctgtact ctatgggtga acatccgagc acctctgatc aggataacag
    41161 cttcaaaacg gacgacttca cgttcaacta tggttggacc tacaacaagc ttgaccgtgg
    41221 ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca ttccgggatc
    41281 ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga ttgatgatga
    41341 tcacaaatgg gttgttctgg gtcgtacccg ctggggttat ggtgatggtt taggcggcaa
    41401 agagatgccg ttctatgaga acttctatgc cggtggttcc agcaccgtgc gtggcttcca
    41461 gtccaatacc attggtccga aagcagttta cttcccgcat caggccagta attatgatcc
    41521 ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gatctgtgta aatcggatga
    41581 tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt
    41641 tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata tgggtaccgt
    41701 ttgggataca aactgggatt ccagccaata ttctggatat ccggactata gtgatccaag
    41761 caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg ggccgttggt
    41821 gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac agttccagtt
    41881 taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat
    41941 gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc ctccggtgca
    42001 aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt ctcggtttag
    42061 cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt
    42121 tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaacgag ttcaaaggcc
    42181 gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt
    42241 ccatgaaagc gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga
    42301 cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac
    42361 gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata
    42421 tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca
    42481 ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg gctgatttag
    42541 cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt
    42601 ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa taccgtgagc
    42661 atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt cctttcgcga
    42721 aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg gcgcaaattt
    42781 tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatt gacgcgacgg
    42841 cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac
    42901 tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg
    42961 caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc ggtcagaatt
    43021 gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc aacgatcgtg
    43081 gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc gtggagatcg
    43141 gtgcctgcac taccattgat cgcggcgcgc tggatgacac tgttattggc aatggtgtga
    43201 tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat acggcagttg
    43261 ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg atcggcggag
    43321 ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg ggcatgggta
    43381 tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg ctgcaaccca
    43441 acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg agcaagcgtc
    43501 tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt tgttcgccaa
    43561 actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt gtcgtttctt
    43621 atattttgac aggaagagta tcttgactac taacactcat actctgcaga ttgaagagat
    43681 tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc tggattttga
    43741 agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg
    43801 ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc
    43861 aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc tgtactactt
    43921 cgccggtatt gacgaagcgc gctttaagcg cccggtcgtg cctggcgatc aaatgatcat
    43981 ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt
    44041 cgacggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg aggcctgata
    44101 cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcgat
    44161 tggcgcgaac gctcacattg gtcctttttg tatcgttgga ccccatgtcg aaattggtga
    44221 gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg gccgcgataa
    44281 tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat atgctggcga
    44341 accgacccgc gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg
    44401 tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac tgatgatcaa
    44461 tgcgcacatt gcgcacgatt gtacggtagg taaccgctgc atcctcgcca acaacgcaac
    44521 gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca
    44581 tcagttctgc atcattggtg cgcatgtgat ggttggcggc tgctccggtg tggcgcagga
    44641 cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga
    44701 agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca atgcgtataa
    44761 gctgatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc
    44821 ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa cgcgcggtct
    44881 gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg agaaacctcc
    44941 ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc
    45001 tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg
    45061 gaagaactgg cagtgatggg cattgttgaa gtgctcggtc gtctgcgtcg cttactacat
    45121 attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatgtttt tgttggtatt
    45181 gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg tatcaaaacc
    45241 attcattacg tcagcccgtc cgtctgggcg tggcgacaga aacgcgtttt caaaataggc
    45301 agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta tgacaaatac
    45361 aacgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccatt agatccagat
    45421 aaaaatggtg cccgtgatgt gctggggatc ccttacgatg cccactgtct ggcattgttg
    45481 ccgggcagcc gtggtgcaga agtcgaaatg cttagtgccg atttcctgaa aacggcccag
    45541 cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cgctggtgaa tgccaaacgc
    45601 cgcgagcagt ttgaacgcat caaagctgca gtcgcgccag acctgtcggt tcatttgctg
    45661 gatggaatgg gccgtgaggc gatggtcgcc agtgatgcgg cgctactggc gtcgggtacg
    45721 gcagcactgg agtgtatgct ggctaaatgc ccgatggtag tgggatatcg catgaagcct
    45781 ttcaccttct ggctggcgaa gcgactggtg aaaactgatt atgtctcgct gccaaatctg
    45841 ctggcgggca gagagttagt caaagagcta ttgcaggaag agtgtgagcc gcaaaaattg
    45901 gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccatgcgat gcacgacacc
    45961 ttccgcgagc tgcatcagca gatccgctgc aatgccgatg agcaggcggc acaagccgtt
    46021 ctggagttag cacaatgatc gaatttgtct atccgcacac gcagctggtt gcgggtgtgg
    46081 atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg atccttgacc
    46141 cggcgcgccc aattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg
    46201 cgctctgtga agagatcaaa gagaaagcgt tgagctggag tctgggccgc gcggaacctc
    46261 acgaaatcga cgaactgaat attttacatg cgaccatgct ggcgatgcag cgtgccgtcg
    46321 ctggactgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc ccgaaattac
    46381 cgatgccttc gatggctgtg gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt
    46441 ctatcctggc gaaagtgacg cgtgacgccg aaatggcagc gctggatatt gttttcccgc
    46501 aatatggttt tgcccaacac aaagggtatc caaccgcttt tcatctggaa aaactggctg
    46561 aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc gcactgggac
    46621 ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg tctgaaccac
    46681 gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcctg gccaaaaccg
    46741 caccgctggt aaaaaaggcg gcagcgttgg gtatgccagc actggcgatc accgatttca
    46801 ccaacctttg cggtctggtg aagttctacg gagcgggaca tggcgcaggg attaagccca
    46861 tcgtcggggc ggattttaac gtccagtgcg acctgctggg tgatgagtta actcacctga
    46921 cggtgctggc ggcgaacaat accggctatc agaatctgac gttgctgatc tcaaaagcgt
    46981 atcagcgcgg gtacggtacc gccgggccga tcatcgatcg cgactggctt atcgaattaa
    47041 atgaagggtt gatccttctt tccggcgggc gcatgggcga cgttggacgc agtcttttgc
    47101 gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc
    47161 gctattttct cgagctgatc cgcaccggca ggccggacga agaaagctat ctgcacgctg
    47221 cggtggaact ggcggaagca cgcggtttgc ccgttgtggc gaccaacgac gtgcgcttta
    47281 tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gattcacgac ggctttaccc
    47341 tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt agcgaagagg
    47401 agatgtgcga gctttttgcc gacattcccg aagcccttgc caacaccgtt gagatagcca
    47461 aacgttgtaa cgtaactgtg cgtctgggtg aatacttctt gccgcaattc ccgaccgggg
    47521 acatgagtac cgaagattat ctggtcaagc gtgcaaaaga gggcctggaa gagcgtctgg
    47581 cctttttatt ccctgacgag gaagaacgtg ttaagcgccg cccggaatat gacgaacgtc
    47641 tggatactga acttcaggtt atcaaccaga tgggcttccc gggttacttc ctcatcgtta
    47701 tggaatttat ccagtggtcg aaagataacg gtgtaccggt agggccaggc cgtggttctg
    47761 gtgcagggtc actggtggcc tacgcgttaa aaatcaccga tctcgatccg ctggaatttg
    47821 acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac ttcgacgttg
    47881 acttctgtat ggagaaacgc gaccaggtta tcgagcacgt agcggacatg tacggtcgtg
    47941 atgcggtatc gcagattatc accttcggta cgatggcggc gaaagcggtg atccgcgacg
    48001 taggccgcgt gctggggcat ccgtacggct ttgtcgaccg tatctcgaaa ctgatcccgc
    48061 ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct
    48121 acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg gaaggggtca
    48181 cccgtaacgc cggtaagcac gccggtgggg tggttatcgc accgaccaaa attaccgatt
    48241 ttgcgccgct ttactgcgat gaagagggca aacatccggt cacccagttt gataaaagcg
    48301 acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg ctcaccatca
    48361 tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg
    48421 atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa cgctcggaaa
    48481 ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac
    48541 ctgactgctt cgaagatatg atcgcactgg tggcactgtt ccgccccggc ccgttgcaat
    48601 cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagagatc tcctatccgg
    48661 acgtgcagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac ggcatcatcc
    48721 tgtatcagga acaggtcatg cagattgccc aggtgctttc tggttatacc ctcggtggcg
    48781 cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct aagcagcgtt
    48841 ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct
    48901 tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct gcggcctatg
    48961 ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag tttatggcgg
    49021 cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg gatgagtgct
    49081 ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac catttccacg
    49141 tcaacgacga cggcgaaatc gtgtatggta ttggcgcgat caaaggggta ggtgaaggcc
    49201 cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc
    49261 tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa ctgatcatgt
    49321 ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg ctgggtgatg
    49381 cgttaaaagc ggcagaccaa cacgcgaaag cggaagctat cggtcaggcc gatatgttcg
    49441 gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc caaccgtggc
    49501 cggagcaggt ggtgttagat ggggaacgtg aaacgttagg tctgtacctc acgggacacc
    49561 ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca
    49621 tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg
    49681 ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac cgttccgggc
    49741 ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg ctggaaaaag
    49801 accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt gggcttaaaa
    49861 tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct cgcgggcttg
    49921 ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc cgtcagtctc
    49981 tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg gcggatgcac
    50041 gcgcgcgatt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg
    50101 atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa tacaggaata
    50161 ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa gcgaaaatcg
    50221 attctctgac cgcggttagc cgtcaggatg agaaactgga tattaacatc gatgaagaag
    50281 tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg
    50341 catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg gattacgttc
    50401 gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca gacgataaag
    50461 ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa
    50521 aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt
    50581 accgcaaagc actgcgtctg atgcaaatgg ctgaacgttt taagatgcct atcatcacct
    50641 ttatcgacac cccgggggct tatccgggcg tgggcgcaga agaacgtggt cagtctgaag
    50701 ccattgcacg caacctgcgt gaaatgtctc gcctcggcgt accggtagtt tgtacggtta
    50761 tcggtgaagg tggttctggt ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc
    50821 tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga
    50881 gcgctgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg cgtctgaaag
    50941 agctgaaact gatcgactcc atcatcccgg aaccgctggg cggtgctcac cgtaacccgg
    51001 aagcgatggc ggcgtctctg aaagcgcagc tgctggcgga tctggccgat ctcgacgtgt
    51061 taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac ggctacgcgt
    51121 aatcggcaaa tgttctgaaa agggtcactt cggtggccct tttttatcgc cacggtttga
    51181 gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat tgccattatg
    51241 ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc ggcgctggtg
    51301 gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa gtttatcgaa
    51361 cataacccac gaatttgcgg cgtgattttt gactgggatg agtacagtct cgatttatgt
    51421 agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa cacccactcg
    51481 acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga atatgcgctg
    51541 gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccaacgaata tcttgataac
    51601 attacgccgc cgttcacgaa agccttgttt acctacgtca aagagcggaa gtacaccttt
    51661 tgtacgccgg ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg ctgtctgttt
    51721 tatgattttt tcggcgggaa taccctcaag gcggacgtct ctatttcggt caccgagctt
    51781 ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat cgcgcggacg
    51841 tttggcgcgg aacagagtta tatcgttacc aacggaacat cgacgtcgaa caaaattgtg
    51901 ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaattg tcataaatcg
    51961 ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat
    52021 gcgttgggga ttctgggtgg gatcccgcgc ggtgaattta ctcgcgacag catcgaagag
    52081 aaagtcgctg ccaccacgca ggcacaatgg ccggttcatg cggtgatcac caactccacc
    52141 tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt cccgtcgatt
    52201 cacttcgatt ctgcctgggt gccgtacacc cattttcatc caatctacca gggtaaaagt
    52261 ggtatgagcg gcgagcgtgt tgcaggaaaa gtgatcttcg aaacgcagtc gacccacaaa
    52321 atgctggcgg cgttatcgca ggcgtcgctg atccacatta aaggtgagta tgacgaagag
    52381 gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc cattgttgct
    52441 tcggttgaga cggcggcggc gatgttgcgt ggtaatccgg gcaaacggct gattaaccgt
    52501 tcagtagaac gagctctgca ttttcgcaaa gaggtccagc ggctgcggga agagtctgac
    52561 ggctggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg ctggcccgtt
    52621 gcgcctggcg aacagtggca cggctttagc gatgcggatg ccaatcacat gtttctcgat
    52681 ccggttaaag tcactatttt gacaccgggg atggacgagc agggcaatat gagcgaggag
    52741 gggatcccgg cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa
    52801 accggccctt ataacctgct gtttctcttt agtattggca tcgataaaac caaagcaatg
    52861 ggattattgc gtgggttgac ggaatttaag cgctcttacg atctcaacct gcggatcaaa
    52921 aatatgctgc ccgatctcta tgcagaagat cccgatttct accgcaatat gcgtattcag
    52981 gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg tttgatgttg
    53041 cgggcatttg ataccttgcc ggagatgatc atgacgccac atcaggcatg gcagagacag
    53101 attaaaggcg aagtagaaac cattgcgctg gaacaactgg tcggtagagt atcggcaaat
    53161 atgatcctgc cttatccacc cggtgtaccg ctgctgatgc cgggagaaat gcttaccgaa
    53221 gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagggca acattacccc
    53281 ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggtgttta ccgcgtacga
    53341 gtcctaaaaa tggcaggata acttgccaga gcggcttccg ggcgagtaac gtgctgttaa
    53401 caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg attattgcga
    53461 cggattatgc gatgagcaaa gctttctact gcgatattct tggtttcacg ctgcaaagcg
    53521 aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat gggcaatatg
    53581 tgattgagct tttctcattt ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg
    53641 gcctgcgtca tctggctttt agcgttgatg atatcgatgc ggcagtggcg caccttgaaa
    53701 gccataacgt gaagtgtgaa gccatccgtg tcgatcccta cacgcaaaaa cgcttcacgt
    53761 tctttaacga tccggacggg ctgccgttgg agctgtatga gcagtaaggc ttgtcatcgc
    53821 cgcatttgcc cggtaacgtg cccggcattg ctactgtaaa atcgcaccat catgacactc
    53881 acgctcaata gacaacttct ctcctcacgc cagattctgg tggcctttag cggcggcctt
    53941 gattctactg tcttactgca tcaactggtg cagtggcgga cggaaaatcc gggcgtcact
    54001 ctgcgtgcta tccatgtgca tcacggttta agcgccaatg ctgatgcatg ggtgaaacat
    54061 tgtgaaaaca tctgccaaca gtggcaggtg ccgctggtgg tcgaacgtgt gcagcttgcg
    54121 caagaggggc taggtattga ggctcaggct cggcaggcgc gttatcaggt atttgcccgc
    54181 accttgttac ccggcgaagt gctggttaca gcgcaacatc ttgacgatca atgtgaaacc
    54241 tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat ggcggaagtt
    54301 tcggagtttg ccggaacacg gcttatccgc ccgttgcttg cccgcacgcg gggggaactg
    54361 gagcagtggg cgttagcgca tggtttacgc tggattgaag acgaaagtaa tcaggatgac
    54421 tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg
    54481 cattttgccg aagcaaccgc ccgcagcgcc gcactttgcg ctgaacaaga gagcctgtta
    54541 gatgaactgc tgtcggatga tttagcgcac tgccaaacgc aacagggaac gttaaagatt
    54601 gcgccaatgg tggcaatgag tgatgcccgt cgcgcggcga ttatccgccg ctggctggca
    54661 gggcagaatg caccgatgcc ttcccgcgac gcgttggtga ggatctggca ggaagtggcg
    54721 ctggcgcgag aagatgcttc cccctgttta cgtttgggtg cgtttgaaat ccgccgctat
    54781 caatcgcaac tgtggtggat taaatccgtc actgggcaaa gcgaaaccat tgtgccgtgg
    54841 cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaagtgtaca gcttactgcg
    54901 ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt caaagcgcca
    54961 ggattgctgc atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat ctggcaagag
    55021 ttaggcgttc cgccgtggct acgtgacacc acgccactgc tgttttatgg cgaaacgctg
    55081 attgcggcgg taggggtatt tgtgacgcaa gaaggtgtgg ctgtaggtga gaatggcgtc
    55141 agttttgtct ggcagaaaac gcttagtcaa gtgaaagccg gataagacgc atcaaacgtc
    55201 gcatccggcg aaagtcaatc aggactcgct caccaccacc gtaccgattt ccgggtggct
    55261 aaagctggta attttatcca gacgcaactc gcgggttgca ccagcggctt cgacgaccag
    55321 atattccaca tttttgcggg agactaaatc gctggccttc gcctgcaatt tttcgccatc
    55381 tttcagctca agtgccagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc
    55441 atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc agtaagtttg
    55501 ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt
    55561 cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg
    55621 cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca
    55681 gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa
    55741 ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt attccgttac
    55801 aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc
    55861 gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc
    55921 gcgccatcct tcagaaaccc aggagcgcat gatgctgcgc ctgctggcgt ggctaaaata
    55981 tgccgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg
    56041 gctgcgtaac gatcatttgg gtattgattt gtggattgag ctggggctgc cggatgagcg
    56101 acggattaag aaagcctgca cccaggccgc agaagtggcg ctgtttgcct ataatagtcg
    56161 ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gcgcagtttg ccaatctttc
    56221 cgtctggtat ctggacgacg agcaactggc gaaagtgagc gcctttgccg atcgtactat
    56281 gacgctgcag gcaacgattc aggatggtgt tatctggtta tcggatgata agaataatct
    56341 ggaagtgaac ttaaccgtct ggcaacaacc ttcatgattg tgatttcccg acatgttgct
    56401 atccctgatg gcgagtttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat
    56461 gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc cagcctgcca
    56521 gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag tgacggagtg
    56581 attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc ggcgctggcc
    56641 cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgacg acctacgcgg
    56701 cccacccgtg catcaaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag
    56761 gcgatgcgcg gcaaagtgcg cagcggtcgg gaataaaaag aaggaatgga tggtgaaaaa
    56821 agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat gtaataatcg
    56881 ggccgaagtc gatacgcttt ctccggcaca ggctgccgaa ctgaaaccga tgccgcaaag
    56941 ttggcgcggc gtgctgccgt gtgctgattg cgaaggaatc gaaacctctc tgttcctcga
    57001 aaaagacgga acctgggtga tgaatgagcg ttatctgggg gctcgtgaag aaccttcctc
    57061 cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa ccgatagcaa
    57121 aggtgaaaag tcatattatc gcgcgaaagg agatgcgctg gagatgctcg atcgtgaagg
    57181 taatccgatt gaatcgcagt tcaactatac gctggaaccg gcacaatcca gcttacccat
    57241 gacaccgatg accctgcggg gcatgtattt ttatatggct gatgcggcga ccttcactga
    57301 ttgcgcgacc ggaaaacgtt tcatggtggc gaataacgca gagctggagc gtggctacct
    57361 ggctgcgcgc ggtaacagtg aaaaaccggt attactgtca gtagaaggtc actttacgct
    57421 tgaggctaat ccggataccg gtgcgccgac caaagtatta gcgcccgata cggcaggtaa
    57481 attttaccca aaccaggatt gcagtagttt ggggctgtaa cccgtcttga gacagaaaca
    57541 aatgaaatat tgcctcatgc gctgcgctta tcaggcctaa agcatacaat gcaatatttt
    57601 gaattagcac gattttgtag gccggataag gcgttcacgc cgcatccggc attttgcgca
    57661 cgaggctggc ttttatcact gagtcagtcc aacaaagcga cttgctttaa tataaatccc
    57721 tggcttcagg tgcccaatat actgtaactg cgtttctggt ttaaatgcag agacatcacc
    57781 actacgcata tgcatcagat cggcagggct aatccagcct gattgcgcca gcgtaagcgg
    57841 atgcccggct ttggcaaatg catcagtgac aaattccgaa caaaaccacg actttttgtc
    57901 tccttcgccc acactgctta actgcgcttt cgccaggccg ctgacgcact gttgacgaaa
    57961 atcctcggag aacggattta gtgagcacat ctggcgagtc accataaatg gaataaattc
    58021 gacaatgccg cgatagttat aaccgctatc tttgatttta ttggcgaacg cggtgatttc
    58081 tgtggcttgt tgcggggtaa ggtccgggac tcgtaagacg aaaagcttat cactatgctt
    58141 catggctttt ttaagggaaa caatctggac gccagcgcct gtcgcttcgg caacgttgtt
    58201 atctcccaaa tagattgcaa cgtgactcac agaggaagtg ctgaatacgc ggataccaaa
    58261 tgaggttacc ccaaggcttg aggagaaaag caaatcgccg ggtttgagat ctggttccgt
    58321 tatttctttt attgattgtt cggtgaaaga gctttgatgc tggaatttaa cagcccatgt
    58381 tttcgcctcg gcatttacca ctgtggctga tgagtctggc tggctgatat caacggtaca
    58441 ggccgaaagt aaaagaaagc aggggagaag caggcggcag tacgcctttg gtttatccat
    58501 tttatacaat ccatgtaaaa aagggccccg aaattcagga ccctttctgg catcagcctt
    58561 taatctgttt caccagataa tcgacgatgt caccagtctt aattaactgt ttctcgccgt
    58621 tacgacgata tttatattcg atatcatcgt tgtcgaggtt acggtcgccc agcacaatag
    58681 tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc tctttgcggt
    58741 catccagcag cacttcgata ccttgtgcgc gcagttcgct gtacagtttc tcagcaagtt
    58801 cctgtacgcg gaaggatttg tgcatgttca ttggcagaat cgccacttgg aacggcgcga
    58861 tagcgtcagg ccatacgatg ccgcgttcgt cgtagttctg ctcaatcgcc gctgctacca
    58921 cacgcgttac gccgataccg tagcaaccca tcgtcaggat ttggttacgg ccatcttcac
    58981 cctgtacgga ggctttcagt gcttcggagt acttggtacc caactggaag atgtgaccga
    59041 cttcgatacc acgtttgatc agcagcgtac cctgaccatc cgggcttgga tcgccagcca
    59101 ccacgttgcg gatatcagca atttccgggg tagcgacatc gcgatcccag ttgataccga
    59161 agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc gcaacggtac
    59221 ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga ccggctttaa
    59281 ccacggcacg aatttcttct tcggtcgcaa aagtcagcgg gctggcaacc tgcggcagtt
    59341 tttctgcttt aacttcgttc agctcgtggt caccgcgcac cagcagcgca accagcggga
    59401 agctactgcc ttcaacggct ttaaccagca gagtcttaac cgttttctca atcggcagat
    59461 tgaactgttc aaccagttcc gcgatggtct tcgcgttcgg cgtatcaacc agcgtcattt
    59521 cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt tcaatgttcg
    59581 ctgcatagtc agaagtatcg gagaagacca catcgtcttc tccgctctgc gccagcacct
    59641 gaaattcgtg agaggcgcta ccgccgatag aaccggtatc ggcctgtacg gcgcggaaat
    59701 ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg taggtttcct
    59761 gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat tcgcgggaac
    59821 gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc tgatagaagt
    59881 tcagcggcag ctgtttgtaa gagctcagct cgttacgaat caggtctgta atcacttctt
    59941 catgagttgg gccgagtacg aacggacggt caccacggtc agcaatacgc agcaattccg
    60001 ggccatactg ttcccaacga ccactctctt gccacagttc agatggctga accaccggca
    60061 ttaacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt tcgacttttt
    60121 tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc ttgcggatca
    60181 tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt gtctccttga
    60241 gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg gaaggtagaa
    60301 caggctcaag gcgagcctgg gacaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg
    60361 tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc
    60421 ggtgacgcgc cagcgaacgt taaaatcatg cagccagacg gcataggttt tgcccgtttc
    60481 ctcaccttta cgataggccg ggcgcgggtc ctgcgccagt acttcgcgga taaacagcgt
    60541 taactgcgga taacgcttct tcagcgtcaa aagctgcttt tcgacctctg cggtaaaaca
    60601 caccgccatt tctgccgctg gcgcactttg cgcatagctg gcactggcat cgggaagcga
    60661 ttcagcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga ccagatccag
    60721 actgccgagc ttcagaatca cgctatcttt atggcaaaca acctctttca gctctaccag
    60781 cgacatacca atcgggttag ggcgaaaagt ggagcgcgta gcgaaaacac ccattctggc
    60841 attaccgccg agacgaggcg gacgcacagt cggacgccag ccgccttcca tcgtttgatg
    60901 aaagacgaaa aggatccata aatggctgaa tgcttccagg ccgcgaacgg cgtcggcctg
    60961 gttgtaggga gcaatgagat gtagttcgcc gttggcgctt tttaccagac ctggctggcg
    61021 cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct cgaactggaa
    61081 actgctcatt tcgccgtgat gttaagcgca gaaccgatac atacagcctg acgatagcaa
    61141 cctggcgtac cgctggtgac ttcgcagcta tgcagtaata ccgcattggc tttcattttc
    61201 gaggcgttga tttgcatccg cttacgtgcg gttggaatgc tcggcggaga gtcctgatta
    61261 gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt gccgactaat
    61321 tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc
    61381 tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct gcttaacatg
    61441 gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat agtatttcct
    61501 caatgtatga tcaaaacgtc aatattgaat caggcgcttg taaaaatgac aagacgggca
    61561 agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaatatta ccagccttta
    61621 acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcgtcaga ctgataagcc
    61681 tgaacgaatt tcttcacgtt ttcggcgtct ttgttgtctt cacgcgttac gatcaggttt
    61741 acgtacgggg actctttatc ttcgacaaag ataccgtctt tcgctggagt caggccaatc
    61801 tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc cagagagcgc
    61861 ggcagctgcg gtgcttccag ttcaacaatt ttcagatttt ttgggttctc aacaacatca
    61921 agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac tttttgcagc
    61981 agcagcagtg aacgaccaag gttagttggg tcgtttggca cggcaacctg cgaaccatcc
    62041 tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata aacaaaagtg
    62101 ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag gtacggttta
    62161 tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg cagaacatag
    62221 tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg
    62281 acttctgcaa cctgctgttc ggcaccaaca atcacgccga ctttaatgtg gtttggatct
    62341 ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc cactgccgca
    62401 aaggttttga atttgaacgc cataccttat tccttcttca attatttatg ttgtgttgaa
    62461 cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat taaataaacc
    62521 agaatgacca gcaataccag taccgtattc atcaccgtcg cgttgtagcc gatgtagcca
    62581 tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat cgcggaataa
    62641 ccaaccaggg taatcagggt gatagttgcc gcattcacca gacccggcaa cgcttccggt
    62701 aacagcactt tacggacgat ctgcattggc gtggccccca ttgcgcggga agcttcaatt
    62761 aacccggttg ggatctccag cagagcgttc tcgaccatac gggcaataaa cggtgctgca
    62821 ccaacggtta acggaacaat cgctgcctgc aatccaatcg atgtaccgac aataacgcgg
    62881 gtaaacggaa tcatccatac cagcaagata atgaacggga tggaacggaa aatgttcaca
    62941 atcgcagaaa tggtacgata caacttcgcg ttagcaataa tttgccccgg acgcgtgaca
    63001 taaagcagaa cgccaaccgg cagaccaatc acaaagccaa aaaaaccgga tacgaaggtc
    63061 attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc agacataacc
    63121 cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt gcgtatcttg
    63181 ttgtgtgccg tgcatttcag tcagcatgat gccgaactta acgccaccgg cgtaatccat
    63241 ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg
    63301 ggcatcgacc gattgaccgg taaactccag acgcagcatc ggcacgcagt cagtaaatgg
    63361 ctccgcttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg tcgactgaat
    63421 aaacttatgc gccagcggcg ttttcggatg cgagaacact tcacttaccg tgtcctgctc
    63481 gatcagttcg ccattgctga tgaccgccac gcaatcacaa atgcgcttca caacgtccat
    63541 ttcgtgagtg atcaacagaa tcgtcaaacc cagacggcgg ttgatgtctt tcagcagttc
    63601 gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac acagcaatac
    63661 tttgggattg ctggctaatg cacgggcaat cgccacacgt tgtttctgcc caccggaaag
    63721 attcgacggg tagctatcat gcttatcgcc aagaccaacc aatgacagca attccgtcac
    63781 gcgacgtttg atctcgtctt tcggtgtgtt gtccagctcc agcggcagag ccacgttgcc
    63841 aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa tctggcgacg
    63901 agctttggtc aactcggatt ctgacagcgt ggtcaattcc tggccatcga ccagcacgct
    63961 accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct tacccgcgcc
    64021 tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga cgttgttcaa
    64081 cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa gttttatcat
    64141 tgattattta ttatcgtcat taagttagtc gtggcatttc gaatgcctgg aacgggcaac
    64201 gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg tttatgcgaa
    64261 gagcactttc ttgcaggtcg aaacatgcga tactagcgtc acatgcctta ttaaggagct
    64321 ataaaaggtg gcgaagagcg tacccgcaat ttttcttgac cgtgatggca ccattaatgt
    64381 cgatcacggc tatgtccatg agatcgacaa ctttgaattt atcgacggtg ttattgacgc
    64441 catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc agtctggcat
    64501 tgctcgcggt aaatttactg aagcacagtt tgaaacgctg accgagtgga tggactggtc
    64561 gctggcggac cgcgatgtcg atctggatgg tatctattat tgcccgcatc atccgcaggg
    64621 tagtgttgaa gagtttcgcc aggtctgcga ctgccgcaaa ccacatccgg ggatgttttt
    64681 gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg gcgataaatt
    64741 agaagatatg caggcagcgg ctgcggcgag cgtgggaaca aaagtgctgg tgcgtacggg
    64801 taaacctatt acgcctgaag cagaaaacgc ggcggattgg gtgttaaata gcctggcaga
    64861 ccttccgcaa gcgataaaaa agcagcaaaa accggcgtaa tgattaaaag atgagctgtt
    64921 gaaataaaaa tgcatttttc cgcttgtctt cctgagacga ctccctataa tgcgcctcca
    64981 tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc
    65041 ctgaaattca gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg
    65101 agctgaaagc cgcgtcgcaa ctgctcttta acaatttatc agacaatctg tgtgggcact
    65161 cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa taccaagtct caagagtgaa
    65221 cacgtaattc attacgaagt ttaattca
//
LOCUS       DAZKHK010000009        63707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKHK010000009 DAZKHK010000000
VERSION     DAZKHK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.009"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(93..1397)
                     /gene="ndh"
                     /locus_tag="ACHOS5_000591"
     CDS             complement(93..1397)
                     /gene="ndh"
                     /locus_tag="ACHOS5_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP9775471.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(1797..2339)
                     /gene="ycfP"
                     /locus_tag="ACHOS5_000592"
     CDS             complement(1797..2339)
                     /gene="ycfP"
                     /locus_tag="ACHOS5_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP9775472.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(2362..3387)
                     /gene="nagZ"
                     /locus_tag="ACHOS5_000593"
     CDS             complement(2362..3387)
                     /gene="nagZ"
                     /locus_tag="ACHOS5_000593"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP9775473.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEIIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMLVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(3398..4222)
                     /gene="thiK"
                     /locus_tag="ACHOS5_000594"
     CDS             complement(3398..4222)
                     /gene="thiK"
                     /locus_tag="ACHOS5_000594"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP9775474.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNRGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(4203..4844)
                     /gene="lpoB"
                     /locus_tag="ACHOS5_000595"
     CDS             complement(4203..4844)
                     /gene="lpoB"
                     /locus_tag="ACHOS5_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP9775475.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(4858..5235)
                     /gene="ycfL"
                     /locus_tag="ACHOS5_000596"
     CDS             complement(4858..5235)
                     /gene="ycfL"
                     /locus_tag="ACHOS5_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP9775476.1"
                     /translation="MRKGCFGLMSLGLLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(5238..5597)
                     /gene="hinT"
                     /locus_tag="ACHOS5_000597"
     CDS             complement(5238..5597)
                     /gene="hinT"
                     /locus_tag="ACHOS5_000597"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP9775477.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSTEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLTHKGL"
     gene            5932..8121
                     /gene="fhuE"
                     /locus_tag="ACHOS5_000598"
     CDS             5932..8121
                     /gene="fhuE"
                     /locus_tag="ACHOS5_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP9775478.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQNDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTFKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(8181..9614)
                     /gene="ptsG"
                     /locus_tag="ACHOS5_000599"
     CDS             complement(8181..9614)
                     /gene="ptsG"
                     /locus_tag="ACHOS5_000599"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP9775479.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(9909..10706)
                     /gene="ycfH"
                     /locus_tag="ACHOS5_000600"
     CDS             complement(9909..10706)
                     /gene="ycfH"
                     /locus_tag="ACHOS5_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9775480.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(10717..11721)
                     /gene="holB"
                     /locus_tag="ACHOS5_000601"
     CDS             complement(10717..11721)
                     /gene="holB"
                     /locus_tag="ACHOS5_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9775481.1"
                     /translation="MRWYPWLRPDFEKLVASYQARRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQAALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(11718..12359)
                     /gene="tmk"
                     /locus_tag="ACHOS5_000602"
     CDS             complement(11718..12359)
                     /gene="tmk"
                     /locus_tag="ACHOS5_000602"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9775482.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(12349..13371)
                     /gene="yceG"
                     /locus_tag="ACHOS5_000603"
     CDS             complement(12349..13371)
                     /gene="yceG"
                     /locus_tag="ACHOS5_000603"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP9775483.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(13374..14183)
                     /gene="pabC"
                     /locus_tag="ACHOS5_000604"
     CDS             complement(13374..14183)
                     /gene="pabC"
                     /locus_tag="ACHOS5_000604"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP9775484.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSITAYPAHYDRLRNEGMMLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(14303..15544)
                     /gene="fabF"
                     /locus_tag="ACHOS5_000605"
     CDS             complement(14303..15544)
                     /gene="fabF"
                     /locus_tag="ACHOS5_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9775485.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(15632..15868)
                     /gene="acpP"
                     /locus_tag="ACHOS5_000606"
     CDS             complement(15632..15868)
                     /gene="acpP"
                     /locus_tag="ACHOS5_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9775486.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(16079..16813)
                     /gene="fabG"
                     /locus_tag="ACHOS5_000607"
     CDS             complement(16079..16813)
                     /gene="fabG"
                     /locus_tag="ACHOS5_000607"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP9775487.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(16826..17755)
                     /gene="fabD"
                     /locus_tag="ACHOS5_000608"
     CDS             complement(16826..17755)
                     /gene="fabD"
                     /locus_tag="ACHOS5_000608"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9775488.1"
                     /translation="MTQFAFMFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEFG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(17771..18724)
                     /gene="fabH"
                     /locus_tag="ACHOS5_000609"
     CDS             complement(17771..18724)
                     /gene="fabH"
                     /locus_tag="ACHOS5_000609"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9775489.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(18792..19862)
                     /gene="plsX"
                     /locus_tag="ACHOS5_000610"
     CDS             complement(18792..19862)
                     /gene="plsX"
                     /locus_tag="ACHOS5_000610"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9775490.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPTGFELLDGGKSGTLR"
     gene            complement(19943..20116)
                     /gene="rpmF"
                     /locus_tag="ACHOS5_000611"
     CDS             complement(19943..20116)
                     /gene="rpmF"
                     /locus_tag="ACHOS5_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9775491.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(20168..20689)
                     /gene="yceD"
                     /locus_tag="ACHOS5_000612"
     CDS             complement(20168..20689)
                     /gene="yceD"
                     /locus_tag="ACHOS5_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP9775492.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            20888..21472
                     /gene="yceF"
                     /locus_tag="ACHOS5_000613"
     CDS             20888..21472
                     /gene="yceF"
                     /locus_tag="ACHOS5_000613"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP9775493.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(21584..22543)
                     /gene="rluC"
                     /locus_tag="ACHOS5_000614"
     CDS             complement(21584..22543)
                     /gene="rluC"
                     /locus_tag="ACHOS5_000614"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP9775494.1"
                     /translation="MKTETPSVKIVAITADDAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            23116..26301
                     /gene="rne"
                     /locus_tag="ACHOS5_000615"
     CDS             23116..26301
                     /gene="rne"
                     /locus_tag="ACHOS5_000615"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP9775495.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAAAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVKPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEDTADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(26496..27449)
                     /gene="flgL"
                     /locus_tag="ACHOS5_000616"
     CDS             complement(26496..27449)
                     /gene="flgL"
                     /locus_tag="ACHOS5_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9775496.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(27461..29104)
                     /gene="flgK"
                     /locus_tag="ACHOS5_000617"
     CDS             complement(27461..29104)
                     /gene="flgK"
                     /locus_tag="ACHOS5_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9775497.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGKDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(29170..30111)
                     /gene="flgJ"
                     /locus_tag="ACHOS5_000618"
     CDS             complement(29170..30111)
                     /gene="flgJ"
                     /locus_tag="ACHOS5_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP9775498.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGNSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(30111..31208)
                     /gene="flgI"
                     /locus_tag="ACHOS5_000619"
     CDS             complement(30111..31208)
                     /gene="flgI"
                     /locus_tag="ACHOS5_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9775499.1"
                     /translation="MIKFLSALILLLVITAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(31220..31918)
                     /gene="flgH"
                     /locus_tag="ACHOS5_000620"
     CDS             complement(31220..31918)
                     /gene="flgH"
                     /locus_tag="ACHOS5_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9775500.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(31971..32753)
                     /gene="flgG"
                     /locus_tag="ACHOS5_000621"
     CDS             complement(31971..32753)
                     /gene="flgG"
                     /locus_tag="ACHOS5_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9775501.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(32925..33680)
                     /gene="flgF"
                     /locus_tag="ACHOS5_000622"
     CDS             complement(32925..33680)
                     /gene="flgF"
                     /locus_tag="ACHOS5_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP9775502.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(33700..34905)
                     /gene="flgE"
                     /locus_tag="ACHOS5_000623"
     CDS             complement(33700..34905)
                     /gene="flgE"
                     /locus_tag="ACHOS5_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9775503.1"
                     /translation="MAFSQAVSGLNVAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNTFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLVFNANGVLTSNPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(34930..35625)
                     /gene="flgD"
                     /locus_tag="ACHOS5_000624"
     CDS             complement(34930..35625)
                     /gene="flgD"
                     /locus_tag="ACHOS5_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP9775504.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(35637..36041)
                     /gene="flgC"
                     /locus_tag="ACHOS5_000625"
     CDS             complement(35637..36041)
                     /gene="flgC"
                     /locus_tag="ACHOS5_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9775505.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(36045..36461)
                     /gene="flgB"
                     /locus_tag="ACHOS5_000626"
     CDS             complement(36045..36461)
                     /gene="flgB"
                     /locus_tag="ACHOS5_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP9775506.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            36616..37275
                     /gene="flgA"
                     /locus_tag="ACHOS5_000627"
     CDS             36616..37275
                     /gene="flgA"
                     /locus_tag="ACHOS5_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9775507.1"
                     /translation="MLTIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVTGQVVSGVVDADGNILIN
                     L"
     gene            37351..37644
                     /gene="flgM"
                     /locus_tag="ACHOS5_000628"
     CDS             37351..37644
                     /gene="flgM"
                     /locus_tag="ACHOS5_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9775508.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            37649..38065
                     /gene="flgN"
                     /locus_tag="ACHOS5_000629"
     CDS             37649..38065
                     /gene="flgN"
                     /locus_tag="ACHOS5_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP9775509.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNSANSVDISQRWQEITVKTQQLRQLNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(38105..39640)
                     /gene="murJ"
                     /locus_tag="ACHOS5_000630"
     CDS             complement(38105..39640)
                     /gene="murJ"
                     /locus_tag="ACHOS5_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP9775510.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(39750..40673)
                     /gene="yceM"
                     /locus_tag="ACHOS5_000631"
     CDS             complement(39750..40673)
                     /gene="yceM"
                     /locus_tag="ACHOS5_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9775511.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALL
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTAPQTAGEQAVRAQRIVDKIWRDAMSE"
     gene            complement(40675..41322)
                     /gene="yceH"
                     /locus_tag="ACHOS5_000632"
     CDS             complement(40675..41322)
                     /gene="yceH"
                     /locus_tag="ACHOS5_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP9775512.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAADGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(41333..41917)
                     /gene="rimJ"
                     /locus_tag="ACHOS5_000633"
     CDS             complement(41333..41917)
                     /gene="rimJ"
                     /locus_tag="ACHOS5_000633"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9775513.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALSAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            42153..43361
                     /gene="mdtH"
                     /locus_tag="ACHOS5_000634"
     CDS             42153..43361
                     /gene="mdtH"
                     /locus_tag="ACHOS5_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP9775514.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            43425..44072
                     /gene="grxB"
                     /locus_tag="ACHOS5_000635"
     CDS             43425..44072
                     /gene="grxB"
                     /locus_tag="ACHOS5_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP9775515.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            44206..44766
                     /gene="yceB"
                     /locus_tag="ACHOS5_000636"
     CDS             44206..44766
                     /gene="yceB"
                     /locus_tag="ACHOS5_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9775516.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPGKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            44872..45918
                     /gene="pyrC"
                     /locus_tag="ACHOS5_000637"
     CDS             44872..45918
                     /gene="pyrC"
                     /locus_tag="ACHOS5_000637"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9775517.1"
                     /translation="MTAPSQVLKIRCPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            45992..46237
                     /gene="dinI"
                     /locus_tag="ACHOS5_000638"
     CDS             45992..46237
                     /gene="dinI"
                     /locus_tag="ACHOS5_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP9775518.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            46527..46781
                     /gene="bssS"
                     /locus_tag="ACHOS5_000639"
     CDS             46527..46781
                     /gene="bssS"
                     /locus_tag="ACHOS5_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP9775519.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            46896..48014
                     /gene="solA"
                     /locus_tag="ACHOS5_000640"
     CDS             46896..48014
                     /gene="solA"
                     /locus_tag="ACHOS5_000640"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP9775520.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            48062..48175
                     /locus_tag="ACHOS5_000641"
     CDS             48062..48175
                     /locus_tag="ACHOS5_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP9775521.1"
                     /translation="MRRLVNYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            48436..49002
                     /gene="yceJ"
                     /locus_tag="ACHOS5_000642"
     CDS             48436..49002
                     /gene="yceJ"
                     /locus_tag="ACHOS5_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9775522.1"
                     /translation="MSFTNTPERYGVISAALHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            49006..49581
                     /gene="yceI"
                     /locus_tag="ACHOS5_000643"
     CDS             49006..49581
                     /gene="yceI"
                     /locus_tag="ACHOS5_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP9775523.1"
                     /translation="MNKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(49623..50675)
                     /gene="trhO"
                     /locus_tag="ACHOS5_000644"
     CDS             complement(49623..50675)
                     /gene="trhO"
                     /locus_tag="ACHOS5_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9775524.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            50900..51820
                     /gene="lpxL"
                     /locus_tag="ACHOS5_000645"
     CDS             50900..51820
                     /gene="lpxL"
                     /locus_tag="ACHOS5_000645"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP9775525.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            51992..53218
                     /gene="mdtG"
                     /locus_tag="ACHOS5_000646"
     CDS             51992..53218
                     /gene="mdtG"
                     /locus_tag="ACHOS5_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP9775526.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKIMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            53301..53675
                     /gene="msyB"
                     /locus_tag="ACHOS5_000647"
     CDS             53301..53675
                     /gene="msyB"
                     /locus_tag="ACHOS5_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFP9775527.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(53676..53903)
                     /gene="yceK"
                     /locus_tag="ACHOS5_000648"
     CDS             complement(53676..53903)
                     /gene="yceK"
                     /locus_tag="ACHOS5_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9775528.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIQGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(54075..56618)
                     /gene="mdoH"
                     /locus_tag="ACHOS5_000649"
     CDS             complement(54075..56618)
                     /gene="mdoH"
                     /locus_tag="ACHOS5_000649"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP9775529.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(56611..58146)
                     /gene="mdoG"
                     /locus_tag="ACHOS5_000650"
     CDS             complement(56611..58146)
                     /gene="mdoG"
                     /locus_tag="ACHOS5_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP9775530.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            58540..59697
                     /gene="mdoC"
                     /locus_tag="ACHOS5_000651"
     CDS             58540..59697
                     /gene="mdoC"
                     /locus_tag="ACHOS5_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP9775531.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(59705..61186)
                     /gene="clsC"
                     /locus_tag="ACHOS5_000652"
     CDS             complement(59705..61186)
                     /gene="clsC"
                     /locus_tag="ACHOS5_000652"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP9775532.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDVMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(61128..61661)
                     /gene="ymdB"
                     /locus_tag="ACHOS5_000653"
     CDS             complement(61128..61661)
                     /gene="ymdB"
                     /locus_tag="ACHOS5_000653"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP9775533.1"
                     /translation="MKTRIHVVLGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(61756..62067)
                     /gene="ymdA"
                     /locus_tag="ACHOS5_000654"
     CDS             complement(61756..62067)
                     /gene="ymdA"
                     /locus_tag="ACHOS5_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP9775534.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(62188..62520)
                     /gene="csgC"
                     /locus_tag="ACHOS5_000655"
     CDS             complement(62188..62520)
                     /gene="csgC"
                     /locus_tag="ACHOS5_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP9775535.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(62579..63037)
                     /gene="csgA"
                     /locus_tag="ACHOS5_000656"
     CDS             complement(62579..63037)
                     /gene="csgA"
                     /locus_tag="ACHOS5_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP9775536.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(63078..63533)
                     /gene="csgB"
                     /locus_tag="ACHOS5_000657"
     CDS             complement(63078..63533)
                     /gene="csgB"
                     /locus_tag="ACHOS5_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP9775537.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15571 a        16931 c        15592 g        15613 t
ORIGIN      
        1 tcagttcata acgcgtctta tcaggcctac cgctcgtacc atttgtccag ggcggataag
       61 gcgtttacgc cgcatccgcc agtacaagcc gattaatgca acttcaaacg tggacggata
      121 acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc atgcagcgca
      181 atctggtgca ttcggtacag cgagatgtat acaaagcgcg caattcgtcc ttcaatcatc
      241 attgagccgc gcgtcaggtt acccatcagg ctaccgactg tggaaaagtt cgacagcgat
      301 accagcgaac cgtgatcttt atactgataa tttttcagcg gcttaccgtt catctgcgcc
      361 agaatgttgt tcattgcaca ggttgccatc tgatgtgcgg cctgagcgcg cggcggaaca
      421 aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtatat gtctggatcg
      481 cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttggt ttcaagacca
      541 ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccacaccat cagatcagcc
      601 tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt gaccatggtt
      661 tgcgtcagca cgcgaacgcc aagtttcgtt aactcactat gggccgcagc agagatacgt
      721 ggcggcaatg caggcaaaat acgttcgccc gcttctacca gcgttacgtt cagggcttcg
      781 ttggtcagac ctttataacc gtaactgtgc agttgcttga ccgcgttgtg caattcagcg
      841 gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgtttacttt gccattcgcg
      901 cccaggttgg cggagtattt caggaacaga ttcagcatct cctggtggaa gcgacgcgcc
      961 tggtgcgggt tatcgaggaa aatgcagttc tctttgacac ctggtgtatt gaaatcgtta
     1021 gaggtgctac ccagcgccat taccagggtg tcataggcga ttttacgttc cggaaccagc
     1081 agttcacctt tctcgtcgcg cagttctgca atagtgattg ttttcgcttc acgatcaata
     1141 tcaattacgg aacccagctg gaactggaaa ccatgattgc gcgcatgggc cagatagctc
     1201 aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag cggtttccac
     1261 aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttgcg tcccagctta
     1321 tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat cacaattttt
     1381 ttcaatggcg tagtcaacgt gaccccctta aatttattaa ccaattgtta attaatggtt
     1441 attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt cgctattgag
     1501 aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt ttgttgctga
     1561 aaagataggc gactttaccg ggtgagatca aaaaacagag tttgttgttt attatttgag
     1621 ctggtttgtg agagtgatat agagtgaggt gtgtatggag ggcattcagt aggcctgata
     1681 aaaggcgcat caggcattga tgctcgactg tcggatgcgg cgtgaacgcc ttatccaacc
     1741 cacagaatcc ggagcgcatc aggtaccggc aaggttacct gatgcgacga gggcatttac
     1801 ccgagggttt tgaacgcttt aatgcgctgt aaatgcgggg agatattctt gaatttgtgc
     1861 gtctgctctt cgtcccagac aatctcgtaa taatgatgca actcttcaga tgtccgctgg
     1921 ctgttaagcg cttcatcatt acgcgacaaa atcaccaggc aacgatcgcg attcttctca
     1981 cggaagttgg tcacacactt agtggcaata tcggcatact cttccgggcg atcaatcttc
     2041 ccttccatgt tctcataagg gaacaaattg gggttgaaga tcacctggcg gatatcacag
     2101 agaaaaccaa tccgctcagc ccaataaccg cccagaccaa caccacaaat gagcgggcgc
     2161 tcgtcgacgt tcagttgcaa cattttgtcc acttctttga gcaaatgctg catatcatgt
     2221 ttcggatgcc gcgtactgta gcttattagc cgtacatccg ggtcaataaa ctgcaattgt
     2281 aagactttct cgtggttacc cggactgtta gagtcaaaac cgtgcaaata gataatcatc
     2341 gcttcctcac ataagccagg gttaatgacc tgccttctct tcctgccagc gttcatgtaa
     2401 ctgattcaga cgggcgctga tcgctttcca gcgagccgag tccatcagtt cctgtcgcga
     2461 aaatgaacct ttatgataca aacgtgtaac acgttctgcc ttgatcggtg acagattatc
     2521 taacacgctg acggcccctt tacgattatt gcagaccagg atcatatcgc aacctgcatc
     2581 cagtgatgcc tgtccacgct cggcataact gcccataatc gctgcgcctt ccatcgataa
     2641 atcgtcagag aaaatcacgc cgtcaaaacc caattcctga cgcaaaacgg ttttcagcca
     2701 gtagggagaa ccgctcgccg gacgcggatc aacatcactg tagatcacat gcgcaggcat
     2761 aatggcgtcg agtttatttt cgcggattaa ggaactgaag accaacatat ctttagcgcg
     2821 aatctccgct tgcggacgtg ggtcgcacgg tgtttctttg tgtgagtctg ccgttactgc
     2881 accgtgacct gggaagtgtt tcccggtagt tttcattccg gcttcatgca taccatcaat
     2941 aaaccggctg gcaattgcca gggctttttg tggatcggca tgataagaac gctcgccaat
     3001 cgccgcgctg atatgcccga cgtccagcac tggcgcaaag ctgatatcaa tatccatagc
     3061 aataatttcg ctggccatca accaacctgc ctcttgcgcc agttttccgc cttcttccat
     3121 tcccaacagc gcagcgaatg attgtgccgc tggcaagcgg gtaaaacctt cacgaaaacg
     3181 ctgcacgcgt ccaccttcct gatctaccgc caccaccaga tgattgcgcg atgctgcgcg
     3241 gatctggcgc accagttcac gtaactgggc aggatcatga tagtttcgcg taaagagaat
     3301 cagccctccc accagcggat gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc
     3361 ttcgacatcc aacattactg gacccacact gctctcctta ttgttttatt aatagctgcc
     3421 gccaggtgtc atcggccagc ctgataaatt gttgatcgcc ggtttgtcgc cagcggtact
     3481 caaaccaccc tgctttgagc atcagcagcc agggaaacca tcgcctgacc tgacgccata
     3541 attgcgccgg ataaatcttc gcgcgagtgg catagtcatt gaccaattgc cgatgctggt
     3601 cagtattttc cacccacacc gccgccagtt ccagcgcgat atcaccatct ccggcatact
     3661 cccagtcgat gagttttaac cctgacgcgc tatgcactaa atttccggcg tggacatcca
     3721 tatgcaatgg gcttaagcgt aaaagccgtg gttcccgcgc tttgcgcaga cgttttaaca
     3781 ttcgcagcca acccactgtc cgccgcgccg gatcgctttg ctgccagtac agttccagta
     3841 acggcaacag cgttattcgc cagccaaaac gcggttgttg atgtagataa tacagcaagc
     3901 ctgccagttc gttggtatcc ggcaaatacg tttttacctc gccgggcaga tagtcgacca
     3961 ccatccagtc acggagatat aaatgcggct tcggtgcaat gcatgcgggt agttgtgata
     4021 aagcccgata ctggcgcaag aacgcggact gcggcgcatc aggatcgtgc ggctgacgca
     4081 caacaaaacg ctggccctga tgttcaatga gaaaactccc gccactaagc cctcgattaa
     4141 acgtcgtgac gggatgaaac tgcgggaaaa agcgcgacag caattcgtcg cgcgtgaggg
     4201 gattattgct gcgaaacggc acctttacct gaccagataa tttcgcccgt ctgcaccagc
     4261 atcagctgca tttgtagggt cggagcgtta acgttgccag aggcgctgga gtacagcacg
     4321 taatgagcgc caacattgcg ggcaatgcct atggctttac tacgggtgcc taaactgtcc
     4381 tgcggcgaca aacctaattg ttgcttagcc atcgacagct gctgggcgga aaccagggta
     4441 aatttaccgt tattagccag cgcatttcgc agcgtttcgg tcgcttctgc ggcattcaac
     4501 gaaccgttag tacggttgtt aacgctatca accagcagga cgctacccgc agtcacgccg
     4561 tcagccccaa gcatcttact gaccatcggc tgcattgcgc cattccagtc ataatggcga
     4621 atatgcggcg caggcggtgc agtttgatct tcgtgctcaa ttgggcctgg ctgctgcggt
     4681 atcgtcggca ccgatggcac tgtggggaca ggctgttgtg gctcggctgg ttgttccggc
     4741 gctggtttca cttcttctac cggtgcaggt tcacgttgcc ccacacaccc ggcaagaaac
     4801 atcgccagcg cggtaatcaa ggcgtagcga ctcatttttg tcatcaagat tcacccctta
     4861 caaatataga taaagtctga ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt
     4921 gaccgccgaa tgtgcgggaa tggtaacgct gcgtggcctt tccagaggat gcatctccag
     4981 ccctctggcg tcataccagt aaaaacgata atgaacggta acgggttctt gcctttcgtt
     5041 ataaagcgtt gaggatgctg aaggttgaat atcagatgtc gaaaggacgg gcttttctgc
     5101 actgatgccc gcagccaata aagatgactc catcaccagc gattgctcat cattcaccgg
     5161 aatttccgga tgtgaacgac agcccaccag cactaacaac cccagagaca tcagcccaaa
     5221 gcatcctttt ctcatcgtta cagaccttta tgcgtcagca ttggtcccag tggacggcca
     5281 cccaacaaat gcatatggat gtggtaaacc tcttgtccgc catggcggtt ggtgttcatg
     5341 atcagacgat agccatcttc ggcaatacct tcttgctcag caatttttgc cgctacggtg
     5401 atcatgcgtc ccagcgcctg ctcatgctca gttgacacgt cgttcacagt cggaatgagg
     5461 atattcggaa tgatcagaat atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt
     5521 accagatcat cctggtagac gatatcggag gggatctcac gacgaataat tttgctgaat
     5581 atagtttctt ctgccacgac attttccttt ttcaaatagc catgcgttgc gccatgctac
     5641 gccgcgccac actgcgagta tagagtatgg tcgagttaaa cctgctcttt caaccttaac
     5701 tcacgatttc ttaagcaaaa aatcgttttg ctgccgtata tatcgccatt attcccattt
     5761 ctgcctgcga atgcgctgaa aagacattct ctgtttggtt tacactccaa atatgaatgc
     5821 gtatatttct catttgcatt tacacacaaa aattattcgc acactaaata taaccgctca
     5881 tcaccctgct tcggcatgag cctgacgcca acataaacca agagatttca aatgctttcg
     5941 acacaattta acagggataa tcaacatcaa gccatcatca aaccgtcact ccttgccggt
     6001 tgcatagcac tggcactatt accttccgcc gcttttgctg caccagccac tgaagaaacg
     6061 gtgattgttg agggttcagc cacagcccca gatgatggcg aaaatgatta caacgtaacg
     6121 tctacctctg cgggtacaaa aatgcagatg actcaacgtg atattcctca gtcggtcact
     6181 attgttagcc agcagcggat ggaagatcag cagttacaaa cgctgggcga agtgatggaa
     6241 aacacgctgg ggatcagcaa aagtcaggcg gattccgatc gtgctcttta ttattcccgc
     6301 ggattccaga tcgataacta tatggttgat ggtatcccca cctattttga atcgcgctgg
     6361 aatctgggcg acgcactttc tgatatggca ctgtttgaac gcgtagaagt agtgcgtggc
     6421 gcgacaggcc tcatgaccgg gacgggtaat ccatctgcgg caattaatat ggttcgaaaa
     6481 cacgcgacca gtcgtgaatt taaaggcgat gtctcggcgg aatacggtag ctggaacaaa
     6541 gaacggtatg tggcggattt acaaagccca ctcaccgaag acggtaaaat ccgcgcacga
     6601 attgtcggcg gctaccagaa taacgactca tggctggacc gctacaacag tgaaaagacc
     6661 ttcttctccg gcattgtcga tgctgattta ggcgatctta cgatgctttc agccggttac
     6721 gaatatcagc gtattgatgt taatagccca acctggggtg gtttaccgcg ctggaatact
     6781 gatggcagca gcaacagtta cgatcgcgca cgcagtaccg cacctgactg ggcgtacaac
     6841 gataaagaga tcaacaaggt ctttatgacc ctgaagcagc ggtttgctga tacctggcaa
     6901 gcgacgctga atgccaccca ctctgaagtc gaatttgaca gcaaaatgat gtatgtcgat
     6961 gcctacgtaa acaaagcgga tggtatgctg gttgggccat acagcaatta tggacctggc
     7021 tttgattatg tcggcggcac cggttggaac agcggcaaac gtaaagttga tgcgctggat
     7081 ctgttcgctg acggtagtta tgaattgttt ggtcgtcagc acaatttaat gtttggtggc
     7141 agttacagca aacaaaacaa tcgttacttc agttcatggg ccaacatttt cccggatgaa
     7201 attggcagtt tctacaactt taatggcaat ttcccacaaa ccgactggtc accacagagc
     7261 ctggcgcaga acgataccac acatatgaaa tcgttatatg ctgccactcg tgtcaccctt
     7321 gccgatccgc tgcatctgat cctcggcgca cgttatacca actggcgagt tgatacgctg
     7381 acttacagca tggagaaaaa ccacaccacg ccttatgctg gactggtgtt tgacatcaat
     7441 gacaactggt cgacctacgc cagctatacc tctattttcc agccgcaaaa tgatcgtgac
     7501 agttcaggca aatatctgac tccaatcacc ggtaacaact acgagctagg tctgaaatcg
     7561 gactggatga atagccgtct gaccaccacg ttagccatct tccgtattga gcaggataat
     7621 gtcgctcagt ccaccggtac acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg
     7681 gtggatggga cagtcagtaa aggtgtggaa tttgaactca acggcgcaat taccgacaac
     7741 tggcagttga cgtttggcgc aacgcgctat attgccgagg ataacgaagg aaacgccgtt
     7801 aatcctaatc tgccacgcac cacgtttaaa atgttcacca gctatcggtt gcctgtcatg
     7861 ccagagttga cggtcggcgg tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg
     7921 acgccgtatg gcaccttccg cgccgagcaa ggtagctacg cgctggtgga tctcttcacc
     7981 cgctaccagg tgacgaaaaa cttctcgtta caggggaatg tcaataacct gttcgacaaa
     8041 acctacgata ccaacgtgga aggttctatc gtctacggcg caccgcgtaa tttcagcatt
     8101 accggcacgt atcaattctg aataacacct gtaaaaaagg cagccatctg gctgccttag
     8161 tctccccaac gtcttacgga ttagtggtta cggatgtact catccatctc ggttttcagg
     8221 ttatcggatt tagtaccgaa aatcgcctga acaccagaac cagcaaccac tacgcccgct
     8281 gcgcccagtt tcttcaggcc agcctgatcc actttagaca catcagcaac gctgacgcgc
     8341 agacgggtaa tacatgcgtc gaggttagta atgttttctt taccaccaaa tgcagcaacc
     8401 agagccggtg ccatttcgct ggtacctgtc gcttttgcat cttcagtcgc gtcttcacga
     8461 cccggcgttt tcagatccag tgctttaatc agcacgcgga agatggtgta gtaaacaatg
     8521 gcataaccga taccgacgat cgggaacagc cacagtttgc tgctgttacc agacagaacg
     8581 atgaagtcga tcagaccgtg cgagaacgac gtaccgtcac gcatccccag aagaatacag
     8641 attgggaatg ccaggcctgc cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac
     8701 atgaaggaga actcgatcgg ctcggtgata ccggtcagga acgaggtcag cgccgcggag
     8761 atcataatac cgcccacttt cgcgcggttt tctggtttag cagagtgcca gatagcaatt
     8821 gcggcagctg gcagaccgta cattttgaac aggaagccac cagacagttt acccgcagtc
     8881 gggtcacccg ccatataacg cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg
     8941 tattcaccaa tctgcatctg gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc
     9001 aggcaacgtt cgatgaaacc gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc
     9061 cactgagaga aggtctggat tgcagaacca atcggcggcc aaatgaagga cagcacaacg
     9121 ccagtaaaga tggcagccag gcctgaaatg atcggcacaa agcgtttacc ggcaaagaag
     9181 ccaagatact caggcagctt aatacggtag aaacggttaa acatgtacgc tgcgatcgca
     9241 ccggagataa tccccccgag tacgccagta tccgccaggt gtttagaggc gatttcttca
     9301 gcaggtaaat gcagtaccag tggcgcaacc acggccatgg ttttaaccat gatgccatag
     9361 gcaacaactg cggccagcgc ggatacgcca tcgttattgg taaagccgag ggcgacaccg
     9421 atcgcaaaaa tcagtggcat gtttgcaaag acggaaccgc ctgcttctgc cataacatgc
     9481 gatacaacgg cgggcagcca gctgaaattc gcggaaccga cgcccagcag aatacctgcg
     9541 ataggcagta cggataccgg cagcatcagc gatttaccga ccttttgcag gttagcaaat
     9601 gcattcttaa acataattga gagtgctcct gagtatgggt gcttttttta cgttctcacg
     9661 cgtggcaagg ggggagtgcc tcgccgtgta cagggcatct aagcgccctt tatttattac
     9721 acagagtaaa ataattcagt gccgatttgt ttgacggcta tcacgtttca acttcagatc
     9781 attggagaag ttgcacattt tgacaaaaca tatgtcacaa tatttcaaac gtggttattc
     9841 accgcccgat tttcggcggt gaactttact cgttttagcc attaattacg agctttaaaa
     9901 aaactcattc aacggatgga ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag
     9961 ttatcggtgg ttacctgcgc cagttcttca acggcaacac ctttcaacac agccatgtat
    10021 tctgcaacgt cacgaaccat cgcaggttga ttctcttttc ctcgatgcgg tactggcgca
    10081 aggtaaggtg agtcagtttc caccagtaac cgatccaggg ggacataacg cgcagcatcg
    10141 cgcagttgct ccgcattacg gaaggtcaca atgccggaaa aggagatgta aaatccgaga
    10201 tccagtaatt tgcccgccgt ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa
    10261 tccgtcactt tttcttcgcg cagaattgcc agcgtatcgg cgcgggcgtc acgggtgtgg
    10321 acgataaccg gcttgttcag ttcacgacca atctggatat gatggatgaa ggactcttgc
    10381 tgacgtactt tagtttccgg cgtgtaataa taatccagcc cggtttcacc cagcgctaca
    10441 acgccctctt ctgccgccag acgacgtaaa tcttctacat cgtagggatc attctggttg
    10501 agtggatgca cgccacaaga aaatacgacg ttgtcgcgtt cgcctaccag atcccgcatg
    10561 tgtaaataac ccggtaatgt tgtggcgact gccagacaaa atttcacatc gcgtgcggcg
    10621 gctttcgcca gaacgtcatc cacgtcctta tgcaaagatt cataatccag accatcgaga
    10681 tggcagtgtg agtcgactaa aaacatgatg tctctcttaa agatgaggaa ccggtagcac
    10741 aacgcccggt tgcaggtaat gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg
    10801 gttgatgcct gtgacagaca ttaactgttc acgaatgtgg caaacatccc ccagtatagc
    10861 ctgcaggcgc gagggagaaa gatggtttgc cagttcgacg accaggcccg gcacatcaac
    10921 attggtcacc tgcgcagcgc catgatggcg ttttagcgca tccatcagca acgttgccag
    10981 ccagtgtaaa cgtgccggag cttgttcatg attaagggcc gctagcagcg aataccagtc
    11041 gcccgattgc acgctatatg ccagcgcctg acacaatgtt tcacgagcct gccagttatc
    11101 tccctgaaac aacgccagtg ccgccccagg cgaaccggcg cttaagcgca atgcggcaag
    11161 tagtgcagcc tgtgacattg tcacttcgcg tgaaagccag gtcacggcgt actgttccgg
    11221 cggcggcgca aggtaatgta accgacaacg actacgtaat gttgccagta aacgttctgg
    11281 ctcgcgggta gcaaggaaaa accacgtctc tgctggtggc tcttcaagcg ttttcagcaa
    11341 tgcgttagcc gcggcgtcgg ttagtaaggc agcatcggtt acccagacaa ctttcgcacc
    11401 acctaagcgt gcgtgctcat tcagcttttc ggtgacctca cgtaccgcat caatgcccag
    11461 cgcatttttc cctttttcgg gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat
    11521 caactgacat ccacgacagt gaccgcaact tttgtggccc tgcggttgtt ggcagagtaa
    11581 ataacggctc agggcataga ttaaagcatc atcgcccatg cccggtaacg cctgaatgag
    11641 tagcgcatgg tgacctcttc tggcctgata gctggctacc agtttttcga aatcaggtcg
    11701 taaccatgga taccatctca tgcgtccaac tccttcaccc agttggtcac ggtagtgcgg
    11761 attgcatcca tcacggcctc cagcggctgg gtggcatcaa tggtatgaat gcttttatct
    11821 tgtgctgcca gttccagata gcgggcgcgg gtgcgattaa agaaatcgaa tgactcttgc
    11881 tcaatacgat ccagctcacc acgggcgcga gcacgtttta agccgacctc tggggtgaca
    11941 tcaagataaa gcgttaagtc agggcgaaaa tccccgagaa cagcatcacg cagtgttgcc
    12001 agcatatgtt ggtcaatacc tcgtcctcca ccctgatagg cttgagtaga gagatcgtgg
    12061 cgatcgccaa tcacccaggt gccgttagcc agcgcaggtt tgatgaccgt ttctaccagt
    12121 tgaacgcgcg cggcataaaa catcagaact tcggctttat cggtaatgac ttcatcgcct
    12181 accgatttga tatccagcac caggcttctt aacttttcgg caagttgcgt accgccaggt
    12241 tcccgagtga aaaccatgtc gcggataccc agttgctcaa gcgtctcaac caccacgtta
    12301 cgcgcggtag ttttgcctgc gccttccagc ccctcaatga cgatatactt actgcgcatt
    12361 tttttcctta agcactttca gataatcctg cacagacttg ttatgactgg caagattggt
    12421 attaaacgtg tgaccacctt taccatcggc cacaaaatag agatacggcg tttttgccgg
    12481 atgcgcagca gccttcagcg aatccgcccc cggcgtagct attgcacctg gcggcaaacc
    12541 ggtaatggta taggtgttat acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc
    12601 attataacgc tctcccatcc cgtaaatcac ggtcgggtcg gtctgcaggc gcataccaat
    12661 gcgtaaccgg ttgataaata ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc
    12721 tttttcgata attgatgcca tcgtcaccaa ctggttttta tctttataag gcagaccgtc
    12781 cgcacgccct tcccaggcgc tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag
    12841 taacgcgaca tcggtggtat tggcggtata catccaggtg tctggccaga accaaccttc
    12901 aatccactcc gggttttcca gttcaagtgc ctgcgctacg gtggcgtact tatcgtcgct
    12961 cagcgtgtgc ttgatatacg gagcctcacg caattgcttg aggtagtcgc tcagacgcat
    13021 cccttctacc agtcgcagcg ggaactgtgc ttctttaccg ctttccaaca atttcagcat
    13081 ctcgcgcacg gtcatctgcg gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc
    13141 cggttcgata cgcagcaacc attgaaaaac ccgtgggcga ttgatgatct tatcggcata
    13201 aagctgttca cctagcgcca gacgtccggt ccctggctta agggtaaata tcgtctcttc
    13261 tttgataagc aatttgctgt cggcaagatg gcgaaccttc cagacgccca caccagcggc
    13321 gatacccagt accaccagca ataacaaaat tatcaataac acttttttca tgactaattc
    13381 gggcgctcac aaagtggggc taaatattca tataacgttg ctgacgaaaa ggagacatcg
    13441 ccacaggcac gcacgggcat cactggcatt aacgcattac aaataaccat ctcatctgcc
    13501 tgcaacgcct cttccagaga ggcttgcact tcgacaagct gataagagga ttgtgccagc
    13561 aaacggatac agaattgtcg cataatgccg ttaacacctg cctgatccag tcgcggcgta
    13621 taaactacgt tgcccttccg ccagaacaaa ttagccgcac agcattccgt aacccaccct
    13681 tcgctgtcaa ggaccagcgc ctcatcagcg tttgtctgct caagatgaga gcggatcaat
    13741 acttgctcaa gccgattaag atgtttaata cctgcaaggt gaggattgcg ccccagccgc
    13801 accgggctta gcgccaacat catcccttcg ttacgcaaac ggtcgtaatg tgcaggataa
    13861 gccgtaatgg agagaatccg cgttgccggt ccactgttca atgtgctgta ccctcgcccg
    13921 ccactaccgc gactgatcac gactttcagc acaccattct gctgttctgc tgccagcgtt
    13981 ttcatctctt gttcaagctg aggccagaag tcacaggaaa tcatcaaccg ctgacaagca
    14041 tcctgtagtc gctggatatg cgccgataac aaactgactt taccgtcgat aactctggcg
    14101 gtggtaaaac aaccatcacc aaactgcgtt gcccgatcgc ttactgccag cgattcctgc
    14161 ttatgaccgt taattaagaa cataccggct ccttatcaga gaattgttag tgtggcagca
    14221 tgttcactac ggaacaagtc ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt
    14281 tataagggtg gaaaatgaca atttagatct ttttaaagat caaagaacca ttagtgccac
    14341 cgaagccgaa ggagttacac agagtgtatt ccattccgct aacctgacgc gcttcgtgcg
    14401 gtacgaaatc cagatcgcaa ccttcatccg ggttatccag gttgatggtt ggcggaacag
    14461 cctgatcgcg cagcgccagg atggagtaga tagattctac tgcacccgcc gcacctaaca
    14521 ggtgaccggt catagatttc gtggagctta ccaacacgcg gcttgcagct tcaccgaaga
    14581 tggttttcac cgcctgcgct tcagctttat cgccagccgg cgtagaagta ccgtgcgcgt
    14641 taacgtagcc aatctgactt gcttcaatgc ctgcatcacg cagagcattt gccatcgcca
    14701 gagctgcgcc tgcgccattt tctggcggtg acgtcatatg ataagcatcg ctgctcatac
    14761 caaagccgac gagttcagcg taaattttcg caccgcgttt tttcgcgtgt tcgtactctt
    14821 caagtaccag cataccggca ccatcgccca gtacgaaacc atcacgctct ttatcccacg
    14881 ggcggctcgc cgcttgcggg ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac
    14941 caccaacgcc cagcggcgta ctggctttct ctgcgccacc tgcaaccatc acgtcagcat
    15001 cgccatacgc gataatacgc gcagcatggc caatgttgtg cacgccggaa gtacaggcgg
    15061 tcgcgataga gatgcttggg ccacgcaggc catacatgat agtcagatga cctgccacca
    15121 tgttcacaat cgttgacgga acgaagaatg ggctgatctt acgtggacca ccgttcatca
    15181 gagatgtgtg gttttcttcg atcagtccga ggccgccaat cccggagcca attgcggcac
    15241 caatgcgggt tgcgttctct tccgttattt caaggccaga atcctgcatg gcctgaacgc
    15301 cagcgacaat tccatattga atgaaggcat ccatcttgcg ctgttctttg cgcgagataa
    15361 tgtcctcaca gttaaaatcc tttactaagc cagcaaattt cgttgcatag gcgctagtat
    15421 cgaaatggtc gattaggctg atgccactct gaccggcaag cagagctttc caggtagact
    15481 ctacggtatt gccgacagga gacaacatgc ccagtccggt cacaactaca cgacgcttag
    15541 acacgtttgt cctccaggga gggaaaaaat gattctagtg ggacaaaaag ataaaactca
    15601 ggcggtcgaa cgaccgcctg gagatgttca cttacgcctg gtggccgttg atgtaatcaa
    15661 tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca gtatcaaact
    15721 cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa
    15781 cgaaagaagc attgttggta acttcttcct gcttaacgcc caactgttcg ccgataattt
    15841 tcttaacgcg ttcttcgata gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg
    15901 cgatggtttt cgtagtgtat aaaatgttga aaaatttgca actaaatccc ggcaggtctt
    15961 accacgattt tacgttattt tgaggcctta ccccccaata acgcaaatca ttttgcacta
    16021 attattactc ataaccacgc agactgcgcg caacatgagc attttgtgca aatcgcggtc
    16081 agaccatgta catcccgccg ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt
    16141 cggatgccag gaatgcaacc gcgttggcga tttcctgtgc gccgccgagg cgacccgcag
    16201 gaacctgcgc caggataccc gcacgctggt catcgctcag cgcacgtgtc atgtccgttt
    16261 caataaagcc cggagcaaca acgtttacag taataccgcg tgacgcaact tcgcgcgcca
    16321 gtgatttact gaagccgatc aagcccgctt tcgccgcagc gtagttggcc tgaccgccat
    16381 ttcccatggt accaaccaca gaaccgatag tgataatacg accatgacgc tttttcatca
    16441 tagcgcgcat taccgctttt gacagacgga aaacagatga aaggttggtt tcgataatat
    16501 cgttccactc ttcatctttc attcgcatta acaggttatc acgagtgata ccggcattat
    16561 tgaccaggat atccacttca ccaaattctg cgcgaatttt ttccagaaca gattcgatag
    16621 atgccgggtc ggtcacattc aacatcagac ctttgccgtt ggcacctaaa taatcactga
    16681 tcgcctgagc gccattttca ctggtcgcag tgccaataac tttcgcgcca cgggctgcga
    16741 gcgtttcagc aattgcgcgg ccaattccgc ggcttgcacc ggttaccagt gcgatttttc
    16801 cttcaaaatt catgattttc ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt
    16861 cgttcagcgc cgaggcggtc agggtgtcga caatgcgttt cgtcaggcca gtaagcactt
    16921 tgcccgggcc gaattcatag agatgttcta cgccttgcgc tgccatgtac tcaacagact
    16981 tcgtccactg aaccgggtta tacaactgac gtaccagtgc gtcacggatg gcatcaccat
    17041 tggtttcgca tttcacatca acgttattca caacaggaac tgttggtgcg ttaaaggtga
    17101 ttttcgctaa ttctactgcc agtttgtcgg ctgctggttt catcagcgca cagtgagacg
    17161 gtacgctcac tggtaacggc agagcgcgtt ttgcgcccgc agctttacag gcagcgccag
    17221 cacgctcaac tgcttcttta tgaccggcaa taaccacctg tcccggagag ttaaagttta
    17281 ccggagaaac gacctgacct tctgcagctt cttcacacgc tttcgcaata gacgcatcat
    17341 ccagaccgat gattgccgcc atagcgcccg tgccttccgg tacggcttct tgcatgaact
    17401 tgccgcgcat ctcaaccaaa cgcaccgcat cagcgaaatc aattacacca gcgcaaacca
    17461 gcgcggagta ttcccccagg ctgtgaccgg ccatcattgc cggtgcttta ccgccctgct
    17521 gctgccatac gcgatacagc gcaacagatg cagtcaacag cgctggctga gtttgccagg
    17581 ttttattcag ttcttcagct ggcccctgct gggtcagcgc ccacagatcg tagcccagcg
    17641 ccgcagaagc ttctgcaaac gtttcttcga caattggata gctcgccgcc atatcagcca
    17701 gcattccaac ggtttgagaa ccctgtccag ggaacataaa tgcaaattgc gtcatgtttt
    17761 aatccttatc ctagaaacga accagcgcgg agccccaggt gaatccaccg ccaaaggctt
    17821 caagcagaac caactgtccc ggcttaatgc gcccgtcacg tacagcttca tccagcgcgc
    17881 acgggacaga ggccgcagag gtattaccgt ggcgatccag cgtcaccacg acattgtcca
    17941 tcgacatgcc gagttttttc gccgttgcac tgataatacg caggttagcc tgatgcggaa
    18001 ccagccagtc cagttgagaa cggtcaagat tattcgccgc cagcgtctca tcaacgatgt
    18061 gcgccagttc cgttaccgca accttgaaga cttcgttgcc cgccatcgtc agatgaattg
    18121 aattctctgg attcacacgg tcagcattag gcagcgtcag caattcacca tagctaccgt
    18181 cggcatgcag atgggtggag atgattcccg gctcttcaga ggcagccagc accgcagcgc
    18241 ccgcgccatc gccaaaaata ataatagtcc cacgatctgt tggatcgcag gtgcgcgcca
    18301 gtacatcgga accgacgacc agagcatact tcaccgcccc agatttcacg tattgatcgg
    18361 ctacgcttaa tgcataggtg aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac
    18421 ctttaatgcc caacatgctt tgaatctgac aagctgcgct cgggaaagcg tgcgtcgcag
    18481 aagtcgttgc cacaacgatc aggccaatct ggtctttctc aatacccgcc atctcaattg
    18541 cgcgtgtcgc cgcttcaaag cccatggttg aaacggtttc gtttggcgcg gcaatgtggc
    18601 gttcgcggat accggtacga gtgacaatcc actcgtcaga ggtatccacc attttttcca
    18661 aatcggcgtt tgtccgcact tgttcgggca gatagctgcc agtaccaata atcttcgtat
    18721 acatgtacgc tcagtcactt ttcggttata taccgtcact tgcaaactgc gagttcgctg
    18781 gcagcgtcct gctaccgcag agttccgctt ttgccaccgt ccagcagctc aaaaccagtt
    18841 gggtatacag attccaggcg agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc
    18901 actgcctgtt caatcgcgac cgcaaaagct cgctgattgg ctgcaccatg actttttatc
    18961 accgtgccgc gcaatcctaa cagacaggcg ccgttatact ggtcggggtt gaggtgactg
    19021 aatcgcctcg tcaggctctt ttgtagccaa cgctttaata acagtagcca ccacgaccgt
    19081 tttttccctt caccctgaga tttcagcaga gaaaggaaca tcctgacaac accttccatc
    19141 gtctttaatg tgacatttcc tgtaaagccg tcacaaacca gcacatctgt cttgccagtt
    19201 aacaactcat tggcttcaag atagccgata taattgatag aagggattgt tttaagcacc
    19261 gctgaggcat cccgaatact gtcgagaccc tttacttctt cttcaccaat attgagcaac
    19321 gccacgcgag gattaggaat ttccaccacc tcttcagcca gaactgagcc cataatggca
    19381 aattgcacca acattgtact gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt
    19441 ttgccctttt gctgatgcgg taataccgtc accagcgccg gacgctcaat cccctccagg
    19501 ggcttgagta ataattttgc cagccccatc agcgccccgg tattaccggc actgacacag
    19561 gcttgcgctc gaccttcttt caccagctcc agggccacgc gcattgaact cccacgactg
    19621 gcgcggatag cttgcgaagg ccgggcatca ctggcgataa ctgactgcgc aggaataatc
    19681 tgcagacgcg aacgttgttc aaagtcagct ttagcaagta atggcgtgat ggcgtcggga
    19741 ttgccgacta aaagaagagt gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc
    19801 actgtcacgg aagggccaaa atcccctccc atgacatcta acgccagggt tagacgtgtc
    19861 aaggtatcgt cgccgcccgg tttggtcttt ccccagttgc ctggggaaaa tcctcactaa
    19921 gcttcatcac gcagacgcgt gattacttag cgatgacctt gcggccgcgg tagtaaccgt
    19981 cggcagtgat gtggtgacgc aggtgttttt caccagaagt tttgtctaca gacaggctgg
    20041 tgactgcggt cagcgcgtca tgggaacgac gcatgccacg tttggaacgg gttggtttat
    20101 tctgttgtac ggccatggac cttactcctc aattacggtt tatccccgat ccaacgggga
    20161 gcaccaatta cttacgcttt aagctggcta atacggcaaa tgggtttggc ttttgcgctt
    20221 cttcaggcag ttcaccaaag accatgtccg cttcggacac ttcacagtgt tcagaatcat
    20281 gcaccggaac taccggcaag gcgaggatga tttcatcttc aaccattgca agcagatcga
    20341 tttcaccgaa ttcgttaacc tcaatcggtt catacgcttc cggcagtgct tcagcctgtt
    20401 cgtctgaacg cacaggacta aaacaatacg ttgtgtagac ctgatgagta aacggcttcc
    20461 cgcaacgctg acactcgagc gttaccgtca ccttcgcatc gccgtttaac actgcgagac
    20521 gttggttatc gatagcgaac gacatggagc attccacatc actgtccaca ctgactacgg
    20581 attcggcgac gcgctcaacc tgatcaggag tatagatacc ctggtaatca aggcgttttt
    20641 gagccgtacg aaccggatcg agagtcaggg gtaattttac cttttgcata gggcgcgcat
    20701 attaactttg taacgtcata gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa
    20761 taattcgcac acattgcggg ttactgcttt attttcgttc agtgctgata acgaacatca
    20821 acctgcaaaa cgaagcgcat agtttaaaat ggctatcatc aatacgctat atctgatgga
    20881 aaaaaatatg cctaaactta ttttagcctc cacctcgccc tggcgtcgcg ctcttctgga
    20941 aaaactgcaa atctcttttg aatgtgcagc acccgaggtc gacgaaactc cacgcagcga
    21001 cgaatcaccg cgacagttgg tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc
    21061 acgttatccg gatcatttaa ttattggttc agaccaggta tgtgttcttg atggcgaaat
    21121 caccggtaaa ccgttaacgg aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa
    21181 tatcgtcacc ttctatactg gactggcgtt gtttaattcg gcgaatgggc atctacaaac
    21241 agaagtggag ccttttgacg tccatttccg tcacctgagc gaggcggaga ttgataatta
    21301 cgtgcgtaaa gagcatcccc tgcactgcgc gggtagcttt aagagtgaag gatttggcat
    21361 tacgctgttt gagcgtttag aggggcgtga tcctaacacg ctggttggtt taccgctaat
    21421 cgcgctgtgt cagatgttac gtcgggaagg gaaaaacccg ctgatgggat agttttttgc
    21481 gccggatgcg gttacttcgc atccggcaat cggtgctggc ggcctgatgc ggcgtaaacg
    21541 ccttatcagg ccaaccgccc attatcaggt ttatatgctt gtattagcgc gcgttacgca
    21601 gcttttgcaa acaacgcttc aaaccttcat ccatcggcgc ttcaatacgc atcacctcac
    21661 cggtccccgg atgagtaaac ttcaacgctg cggcgtgcag gaacaggcga tttaatcccg
    21721 tgcctgcttc agtgagctgt ctgtcaaatt cacggtcacc gtagcgatcg tcaaaggcaa
    21781 tcggatgacc cgcatattgt gtatgcacac ggatctgatg agtacgccct gttaccgggc
    21841 tacaacgcac cagggtggca aatgcatagc gttcttccac tttaaagcgt gtttccgacg
    21901 gtttgccttc ctgactcaca cgcacaatac gttcgccgct ttgcagaata tttttcagta
    21961 acggcgcttg aacgctcttc acatgcgact gccactgacc gcgcaccagc gccaggtaat
    22021 ctttttgcat ccctttttca cgtaattgct catgcagaga acgcaacgcc gagcgttttt
    22081 tcgctaccag caacacacct gaggtgtccc ggtcaagacg atgaaccagt tcaaggaacc
    22141 gcgcttccgg gcgcaacgcc cgcaaacctt caataacgcc gaagcttaaa ccactgccgc
    22201 catgtaccgc cgtaccggaa ggtttattca gcaccaggat atgatcatct tcatataaga
    22261 tgacgtccgc cagcgccgcc accttttgca gatgtggcga aaccgcctct tcttcccgct
    22321 cagcaacgcg aaccggtggg atacgcacct catcacccgc ttcgagttta tattcaggct
    22381 taatacgttt tttgttcacc cgcacttcgc ctttacgcaa aatacggtaa atcatacttt
    22441 ttggtacgcc tttcaattgg gtacgcaaaa agttgtcgat acgttgcccc gcgtcgtcag
    22501 cggtgatagc aacaattttt acggatggag tctctgtttt catggtgggc gattctaatt
    22561 agccaacagg attcgcgcca ctcatttttc tatgcttata tttagtttta caccttatta
    22621 cttcactagg tgatcacttt attgatggtt attaaaccaa tcaccagcaa gaagtgaaaa
    22681 aactgtgagt aagcgggtga taaatggtaa aagtcatctt gctataacaa ggcttgcagt
    22741 ggaataatga ggccgtttcc gtgtccatcc ttgttaaaac aagaaatttt acggaataac
    22801 ccattttgcc cgaccgatca tccacgcagc aatggcgtaa gacgtattga tctttcaggc
    22861 agttagcggg ctgcgggttg cagtccttac cggtagatgg aaatatttct ggagagtaat
    22921 acccagtctg tttctttgat aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca
    22981 ctctgcgcct ctttgagctg acgataaccg tgaggttggc gacgcgacta gacacgaggc
    23041 catcggttca cacccggaaa ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat
    23101 aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa ctcagcagga agagttgcgc
    23161 gttgcccttg tagatgggca gcgtctgtat gacctggata tcgaaagtcc agggcacgag
    23221 cagaaaaagg caaacatcta caaaggtaaa atcacccgca ttgaaccgag tctggaagct
    23281 gcttttgttg attacggcgc tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc
    23341 gaatatttcc ctgctaacta cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt
    23401 gaaggtcagg aagtcattgt tcagatcgat aaagaagagc gcggcaacaa aggcgcggca
    23461 ttaaccacct ttatcagtct ggcgggtagc tatctggttc tgatgccgaa caacccgcgc
    23521 gcgggcggta tttctcgtcg tatcgaaggc gacgaccgta ccgaattaaa agaagcactg
    23581 gcaagccttg aactgccgga aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa
    23641 tctgctgagg cgctgcaatg ggatttaagc ttccgtctga aacactggga agccatcaaa
    23701 aaagccgctg aaagccgccc ggctccgttc ctgattcatc aggagagcaa cgtaatcgtt
    23761 cgcgcattcc gcgattactt acgccaggac atcggcgaaa tccttatcga taacccgaaa
    23821 gtgctcgaac tggcacgtca gcatatcgct gcattaggcc gcccggattt cagcagcaaa
    23881 atcaaactgt acaccggcga gatcccgctg ttcagccact accagatcga gtcgcagatc
    23941 gagtccgcct tccagcgtga agttcgtctg ccatccggtg gttcgattgt tatcgacagc
    24001 accgaagcgt taacggccat cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc
    24061 gaagaaaccg cgtttaacac taacctcgaa gctgccgatg agattgctcg tcagctgcgc
    24121 ctgcgtgacc tcggcggcct gattgttatc gacttcatcg acatgacgcc tgtacgccac
    24181 cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa
    24241 atcagccata tttctcgctt tggcctgttg gaaatgtccc gtcagcgcct gagcccatca
    24301 ctgggtgaat ccagccatca cgtctgcccg cgctgctccg gtaccggtac cgttcgtgac
    24361 aacgaatcgc tgtcgctctc tattctgcgt ctgatcgaag aagaagcgct gaaagagaac
    24421 acccaggaag ttcacgccat tgttcctgtg ccaattgctt cttacctgct gaatgaaaaa
    24481 cgttctgcgg taaatgcgat tgaaactcgt caggacggcg tacgctgtgt gattgtgcca
    24541 aacgatcaga tggaaacccc gcactaccat gtgctgcgcg tgcgcaaagg ggaagaaacc
    24601 ccaaccttaa gctacatgct gccgaagctg catgaagaag cgatggcgct gccgtctgaa
    24661 gaagagttcg ctgaacgtaa gcgtccggaa caacctgcgc tggcaacctt tgccatgccg
    24721 gatgtgccgc cagcaccaac gccagctgaa cctgccgcgc ctgtcgtagc cccagcacct
    24781 aaatctgcac cggcaacacc agccgctcct gcccaacctg ggctgttgag ccgcttcttc
    24841 ggcgcactga aagcgctgtt cagcggtggt gaagaaacca aaccgtccga gcaaccaaca
    24901 ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac
    24961 cgccgtgacc gtaatgagcg ccgcgaccct cgtagtgaac gtacagaagg cagcgataat
    25021 cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc agactgccga gacgcgtgag
    25081 agccgtcagc aggcagaggt aacggaaaaa gcgcgtacca cagacgagca gcaagcgccg
    25141 cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc aggcgcaaca agaagtgaag
    25201 gcgctgaatg ttgaagagca atctgttcag gaaaccgaac aggaagaacg tgtacgtccg
    25261 gttcagccac gtcgtaaaca gcgtcagctc aatcagaaag tgcgttacga gcaaagcgta
    25321 gccgaagaag cggtagtcgc accggtggtt gaagaaactg tcgcagccga accaattgtt
    25381 caggaagcgg cagccccacg cacagaattg gtgaaagttc cgctgccagt cgtagcgcaa
    25441 actgcaccag aacagcaaga agagaacaat gctgataacc gtgacaacgg tggcatgccg
    25501 cgtcgttctc gccgctcgcc tcgtcacctg cgcgtaagtg gtcagcgtcg tcgtcgctat
    25561 cgtgacgagc gttatccaac ccagtcgcca atgccgttga ccgtagcgtg cgcgtctccg
    25621 gaactggcct ctggcaaagt ctggatccgc tatccaattg tacgtccgca agatgtacag
    25681 gttgaagagc agcgcgaaca ggaagaagta caagtgcagc cgatggtgac tgaggtccct
    25741 gtcgccgccg ctgtcaaacc tgttgttagc gcgccagttg ttgaagaagt ggctgaagtc
    25801 gtagaagccc ccgttcaggt tgccgaaccg caacctgaag tggttgaaac gacgcatcct
    25861 gaagtgattg ctgccgcggt aactgaacag ccgcaggtga ttaccgagtc tgatgttgcc
    25921 gtagcccagg aagttgcaga acacgcagaa ccggtggttg aaccgcagga agatacggca
    25981 gatattgaag aagttgccga aactgctgag gttgtggttg ctgaacctga agttgttgct
    26041 caacctgccg cgccagtcgt cgctgaagtc gcagcagaag ttgaaacggt agctgcggtt
    26101 gaacctgagg tcaccgttga gcataaccac gctaccgcgc cgatgacgcg cgctccggca
    26161 ccggaatatg ttccggaagc accgcgtcac agtgactggc agcgccctac tttcgccttc
    26221 gaaggtaaag gtgccgcagg tggtcatacg gcaacgcatc atgcatctgc cgctcctgcg
    26281 cgtccgcaac ctgttgagta ataattagct caagtaatca agccctggta actgccaggg
    26341 cttttttatt tcatctttga gtcaacatat taaaattcat caaatggcat ccttgctaac
    26401 caacaataca aaataggcaa aaaaatgccc gccccggcga tcccggagcg ggcagacaaa
    26461 acatacccag tttcatgata tgttttaaag cgagcttatt tgttgagctg gaagagcgac
    26521 aatccctgca tatcggtaaa tgctttatac gatgcctgca atgccgtttg ctgcatgatg
    26581 taagatgaaa tagttgcatt ccagtccaca tcaaccagat cgctcatctg ctgcgtttgc
    26641 cctaaagcgc gatcgctacc taatgaatca agcgactcca gttcgttcag ctgcgtgcct
    26701 aattccgcgc gaacagtcag cacattgttc agcgagtttt tcagtccgcg gttggtttta
    26761 tccagtgccg cagcggcggt ttctttatcc gcttcgctat ccgcgaccgg cgttttcagg
    26821 gctgcgatgg cgctatccag catggcaaaa agattggttt cagaagcgct accgtctggt
    26881 tccgctaccg cgttgctggt aatactgtcg aaaattttgt cgcccgtatg tcctatcacc
    26941 atcgaacgcg aagcatcgac ctgttgttta atactttctg ttccgccgac ataatcaccg
    27001 tcagcctcac taaacggcgc agtctctgtt ttataaccgg caaaaatgta gcgcccgtta
    27061 ccgtcagtgg tgttcgccag attcagtaac tgatcacgaa gcccctgaat atccgtagcc
    27121 agtgaggccc ggtcatcgtc actcaaggtg ccattgctgg cgtagacaat tttttcctga
    27181 gcattctgga tagcagtggt gacctggcta agcacactct cttccagtga cactttttga
    27241 gtggcgaaag tacgcgccag cgtgtactgg ctgttttgtg cctgtgcctg ggagagaaca
    27301 acagcttgtg atgcagcaat gggatcatct gaagggttaa cgactcgctt acccgtcgac
    27361 atctgttcgc cgtacttcat ccattctgcc tgagaattgg tgatgccacg catgttttgc
    27421 tggtacatca tctgggtact gaaacgcatt ttatctcccc ttagcgaatg ttaatcagcg
    27481 catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt cgccaggtaa tactgctgaa
    27541 aacgttgcag atttccgtac tcctcatcga gattgacacc ggaaatcgac tgctgctgat
    27601 tggaaagctg cgtcaccaca ttaccttgcg tggcgctact ggttttcaac gtcgcggttt
    27661 tattcccgat atcactcact aatgaggcat aagcgtcgtt aaaggatttc gcaccgccca
    27721 ccgttttact gttgctttgc agatccagca gggcctgacc gttgcggttg tcgctatcac
    27781 ccgcatcttc ttcgctcgcc atggcgattt tcgcttcgtc agtgattaat acatccatgt
    27841 tgacgatggc gtcacttacc ggtttcagcg tgaagctgtc gttaacggca ggcgttcctg
    27901 taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt ggcatccggc gtcactgtaa
    27961 aagtggtatt gctggcaagg cgggtgacct gccactgatt attatcgaac gagattttgt
    28021 aatctgtcgc cagtaccgcg gaggcatcag ttaccgtggc accgatcgca acgtcaccgt
    28081 tgtttttcgt gttttgcaga accgcgggct taccgatagc aaagaaatct ttaccggcat
    28141 cgccgttggc atcaaatccg gctttgtgtt gggtgttgaa agcctcggca aatgccagcg
    28201 ccagttgtcc gagcgtatta cgcgtctggt ccagatcctg agaacggaat gtcagaatgc
    28261 cgcccagcga cccggtattc agtaatttct ccgggatctc aatattgcca gccgtcccat
    28321 caatataagc gacagtcgta cgagaagggt cggcgctgga aggaactgcc gccagctgcc
    28381 gcgccgtact tccctgaacc agtgagtagc cattggccat cgtgatgtta taagtgccac
    28441 catcctgaac gctaacttct acgccaacaa tctggtttaa ttcactcacc agttgatcgc
    28501 gttgatccag cagattgtta ggtgacgccc ctgcccccac gcctgtcaga cgcgagattt
    28561 gatcgttcag gctggcgatt tgtttagcgt aattgttgat ctgatcaacg ctggcaccta
    28621 tcgcgatatt gacctgtttg tcctggtcgc gcagatattg atcggtggtt ttaaactgat
    28681 tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc tgccgggtct tccgcgttac
    28741 ttaccagcgt ttgcaggctt gtgaagaaat cctgcatctg tgttgccagc gaagaggtac
    28801 tggtggagag catattgtcg attttcgaca tctgctcata gcgggcagtc aggccgctac
    28861 tttgcgtctg cgccgcacgt aactggttgg taataaatgc atcatactca cgctgcacac
    28921 cagaaacgta gacgccattg ccaacccagc cgccagcgcc caacgtgcta ttggcctgcg
    28981 ccataatagt ggtttggcgg gtatatccgg caacgttata gctggagata ttattacttg
    29041 ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat ggcgttattg atcaagctgg
    29101 acatggaggt tccttataag ccttcaatta cagagtatta tcggcagcga cccgccggac
    29161 ttgagttatt cagaacagat tatcaatgtt catactgtag gttttgctca ctttgtcgct
    29221 tatcgatttc atctgctgaa tcatgttggt gagtttgcgg gcatagtgag gatcggtggc
    29281 atagcccgcg tcctgtaggg cctgcgcccc ctgctccgca ctcgcggcgg tcgtcacggc
    29341 ggcgtagcgc gggttacgcg ttaacagccc aacgtaatcc gacaatgctt ccagatacga
    29401 gctgtagacc cgaaatttcg cttttacttt cttcgcttcg ccgttttcat attcagtcgt
    29461 ggtgatttca gtgactggcc ctttccagtt gccagaggct ttgacgccaa acaggttata
    29521 actcggctcg ccgttttcgc ggcggatttg ccgttgcccc cagccagatt caagcgccgc
    29581 ctgagcgagg atcaaatgat gtggcacacc gctttgctgg cttgccagtt gggcgggcag
    29641 tgagagttgc gcgaggaatg ctttactgtt acccggcaac gaatcatcgt agttacgtgg
    29701 tacggccttt tgcaccagct gagaaagcgc ctgattttga taacgcacca cagtttcgag
    29761 cgggaatttc atcggtgctg ctggcgtggg ctcctctggc aatggttgtt ctggcgtcat
    29821 ctgtttaacc atcatctctg caagccccag acctttgccc gccgtcatct gttgggcaat
    29881 ctgctggtca tacatactgg tatacaggcg agtgtgctcg ctgctgaaca ggccatcttt
    29941 tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg aacatccctt ccacctggcg
    30001 ggccaccgga cggatatttg ccgctggatc ttcgcccgct ttcgccttta gttcgttgag
    30061 tgattgcgca tcccaggccg cacttgccag tagtttgctg tcgctgatca tcagatgatt
    30121 tccagttttg cccgcagaca tcccgcactt tgcattgatt gcaaaataga catcagatcc
    30181 atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt tattgaggct ggcgctggaa
    30241 cgtacgcttt gcagcgagcc gccgctctgg cgtaaatcga tctgcgtttg tggagtaacc
    30301 acagtctgcc caccaccaaa cggtgtatct ggctggctga cattggcctg acgattgacc
    30361 gtaaccgaga gattcccctg cgctaccgcg cagctgtcga gggtcacttc gcgattcatc
    30421 accaccgagc cagtgcgcga gttaatcact actttagcgt cctgcggggt gacattaacc
    30481 tgcatattct ggatatcggc aaggaaacgg acctgggaac tgttgccact tggtacgcgc
    30541 acctgaatag tccgcgcatc taacgcggtg gcgctgccat atccacgcac gcggttgatg
    30601 gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt taagttgcaa attaagggta
    30661 ttcccgacgc caaactggct gggcaattca cgttcaataa ccgcaccatt ggtgatccgt
    30721 ccaccgttca gttggttcac ctgaacactg ctaccgccag cggaggctcc agcgccacca
    30781 accagaatat tgccctgcgc cagcgcatac acctgactgt caacgccctt aagcggtgtc
    30841 atcaacaacg taccgccacg caagcttttg gcattaccca tggaagaaac caccacatcg
    30901 atggtttgcc cctggcgtcc aaacggcgga agtgacgccg tcaccattac cgcagcgacg
    30961 ttttttagct gcatattggt gcccgtcgga acggtaattc ccagctgtga gagcatgtta
    31021 ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt caccggttcc atccagcccc
    31081 accaccaggc catagccaat cagtgagttt tgccttaccc cctgaacact ggtgagatcg
    31141 cgaatacgct cagcctgagc cgccgtgata accagtagaa gaattaatgc agagagaaat
    31201 ttaatcacca cagcctcact tacattggcg acaggttaag gaagaaacgc tgcaaccagc
    31261 ccatattttg cgcttcgtta atgtagccat tgcctacgta ttcaatgcgc gcatccgcca
    31321 cctgagtaga tggtacggta ttgctgccgc tgatagtgcg tggattaacc acgccagaga
    31381 agcgaataaa ttcggtaccc tgattaatgg cgatctgttt ttcacccacc acatgcaggt
    31441 tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt accgctaaag gtattgctgg
    31501 cattggcccc gccttttccg ttgaacgtgt taccaccgga ggcttcgaca tcggcacgag
    31561 cgttaccaaa caacccctgc aaatagcgcg gcacagtatc aaagccaaaa ttagttttac
    31621 cgtcacggct ggcattcgca gaggagcttt tgctggcgct gacgttctcc tgcaacacga
    31681 tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc aaacagcggt tgatagccat
    31741 agttaatcgg ctgagcagac tggaaaatag aaccgttggc gacgggcgtc ggaccgggaa
    31801 ccggttgtgc actggtcgcc ccttgtacca gcggcgtgga gggtatccag gcgcagccgg
    31861 ttagtgaaag caccaacaag ctggaaatgg cataagtatg cgcagcgttt ttttgcatgg
    31921 ctatcatctt caaaaatcag taaaccggtg aacctgccac cggttatgcc ttagagttgc
    31981 gtcagttttt gcagcatctg atcggtggtg gacaccgctt tactgttgat ttcgtaagcg
    32041 cgttgcacct gaatcatatt gaccagttct tccgccacat tgacgttaga cgtttcaaca
    32101 tacccttgat acagcagtcc cgcaccgttc aggcctggcg tgctttcgtt cggtgcaccg
    32161 gaggattgcg tttcggtgta gaggttttcg ccaatgctct ccagcccggt gtcattcata
    32221 aaggttgtga gattgagctg cccaacctga accggagctg cctggccttg ttgggttacg
    32281 ctgaccacgc catcacgacc gatggtgata cttaacgcat tcgccggaat ggtgatcgct
    32341 ggctgcacct gaaaaccacc agccgtcacc agctgcccgt tctgatccac ctggaaagag
    32401 ccgtcacggg tataggctga tgaaccatct ggcaacatca cctggaaaaa gccctgccct
    32461 ttaatcgcga catctttgct gttgttggtc tgcgacaggt tgccctggct gtgtaagcgt
    32521 tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc cggagggtaa ggtggtttgt
    32581 tcggaagact gtgcccccgg ctggcgaatg gtttgataaa gcagatcttc aaacaccgcg
    32641 cgctgacgct taaaaccgtt agtactgacg tttgccaggt tgttggcaat gacgtccata
    32701 ttggtttgct gggcgtcaag gcccgttttg gcgatccata atgaactgat cataaaatgt
    32761 cctgtagtgg attagtgaca aagtgcatat gtcttcttgc cggatgcggc gtaaacgcct
    32821 tatccggcct acaagtgctc gcaaaatcga taaattgcga ttgcccccat agacccgata
    32881 aacttgcgca tcgggcaact tatacttgtc atgtatcctt tcaattaact catcgacagc
    32941 agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca
    33001 aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct
    33061 tccagaacac ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg cccacgcgta
    33121 gcctggcttt ctgcgcttaa acgaaaaatg ccatcgtcac cgcgctggac ttcgctgccc
    33181 gtggctttca ccagcttcag acgccccact ggcgcaaccg tatttgccgg atcgcccgga
    33241 ttcagcgccg agattgtgcc atcggcagca atagtgattt ccgccccttc cggcacagca
    33301 attggccccg cctcgcctac caccggatgt ccctgaattg tcagttgccc ggtgggatca
    33361 acctgaatgc tgccattacg cgtataccct tcactgccgt cagcggtctg cacggccagc
    33421 caaccatcct gctgcaacgc aacgtccagc gggcgcgagg tgtaatccat cttgccgggt
    33481 gtcatgtctg cacccggtgt tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct
    33541 tccaccggca ctgcgcgcaa ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg
    33601 gccagattac tggcggttac cgcctgttga ttcagcgtct ggctggctgc tcccatcgcg
    33661 gtataaattg cgtgatccat tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt
    33721 attgaggatc tggtcctggg ttttgatggt ctgggcgtta gactgatagt tacgctgggc
    33781 aacgatcata ttgaccagtt ctttactgag atcgacgttg gacgcttcca gcgcaccgtt
    33841 ggtcagtgtg ccaaagttgc ccgtcccggc tgtccccaac agcgccacgc cagaagattg
    33901 cgtagcggac cagacgttgt cgccttcgga tgccagacct tcgttgttgg caaagttcgc
    33961 cagtacaatc tgccccagca gttgggtttg ttcgttggaa tagttgccga caaccgtacc
    34021 gtcatcattg atttgataac tcaccagatc gcccggtttg tagccgttct gggtggttgc
    34081 cacaatgttg ttagcgccgg tattttgctg catggagttg aggaagctca ggctaaacgt
    34141 ggcgggatct gcgccgttaa ttgcgccagt ggaaatattc gctgttggat ttgaggtcag
    34201 aacgccattg gcattaaaca ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact
    34261 actatcctgg gtatagacct gccagttatt atccccggtc ttcacaaagt agacgctcat
    34321 gtcatgagca ttaccctgac tatcgaaaac agtcaccgaa ccttttttgt tatagctatc
    34381 cgcattgctg gcatcaaatg tgttaacaga gggaagcgga tcactggaat tcaggttgat
    34441 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt
    34501 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt accccgtcag
    34561 ctgtaaacct tgcatattca ccaggttacg gttttcatcc agcttaaatt gtccgttacg
    34621 gctgtagaac accgaaccat tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    34681 aacgtccaga ccacgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    34741 agcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    34801 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    34861 ggtggcggca acgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    34921 tgtgaaggct taaattatct gccgtacttc gtcgagggta gtggtgccgt aagtgccaag
    34981 atccagcgta ttaccgttgt tgccgcggat caccccctgc accagagcaa actgcagcgg
    35041 ttgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    35101 cggcgcagtg ctgccgtcag tcaacgtacc gtcccaggtg aaactgtgga ctccggcagt
    35161 cagttcaccg atatcaatgg tacggacgac cgcgccgttt ttatcggtga tggtggccgt
    35221 cactttgtct gccgcctgtt gcaactcaac accaaacggc gtggtcgtgg tcacagcccc
    35281 ttcttcactg ccggttcccg caagaacagt ggtaccgggg atcatcacgc cgtgaccaat
    35341 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccaagcgt
    35401 ggtattgagt ttttcaatcc cactgaccgt gctgatttgt gccaattgcg acgtcagctc
    35461 gttgttttcc attggattgg tcgggtcctg gtttttcagc tgcgccacca acaaagtcag
    35521 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    35581 gacgccggta ttcgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    35641 tgaccgagcg taagggtttt cagcatcata cttttcaccg tgttgagcac ttcaacattg
    35701 gcctgataac tgcgtgacgc cgacatggtg ttaaccatct ctccgacgac atcaacgttt
    35761 ggcattttca cgtagccctt tgcatccgcc agcggattac ccggttcata aaccagtttg
    35821 tccggggcct gactttctat aacatcggca acctttacgc cgcctgtcgc agcacctggc
    35881 gcagcgttaa cctggaatac cacctgtttt gcccgatatg gctgtccatc gggaccggtg
    35941 acgctatcag cattcgccag attactggcc gccacgttca ggcgctggga ctgggcagtt
    36001 aacgccgacc cggcgatatc aaaaatattc agcagtgcca tccgttaatt tccgctctgt
    36061 aaaacgttca tcatgccttt gatctgcccg ctcaacgcgc taaggctcat ctggtattgc
    36121 aggctgttat cggcaaactg ggtgcgttcg cgatccatat cgacagtatt accgtcaagc
    36181 gaaggctggt ccggaatacg gtattgcagt tctgcggaag gaggcgtcag cgcctgcgcc
    36241 ggaatgtgtt tcgttgaggt catcgtcaga gcaaccacac tggttgcatc ccgtccacgt
    36301 tgcatgactt ttttaagttc actggcaaaa tcgatatcgc gagcctgata accaggggta
    36361 tcggcattgg cgatgtttgc tgccagcact tcctgacgct gggcgcgcag attgagcgcc
    36421 tcttgttgaa aacgtaaggc ggcgtcgagc ttatcgagca tatctcctcc gcaggtatca
    36481 aaattctgcc atcagcttaa atgcctttac gcgcgcgtta tcggcggaat aaacgcaaaa
    36541 tgggtcgcta tttatgccgt tgatggtcat tgcggacagg tacaattcac gttgtagaaa
    36601 tggctggggg cgaaaatgct gacaataaaa cgtagcgtgg cgatcatcgc gatactgttc
    36661 agtccgttaa gtgcggcgag caatctcaca tcgcaattgc acaccttttt tagcgcccag
    36721 ctcgcggggg taagtgatga ggttcgtgtt tctattcgta cagcgcccaa tctactaccg
    36781 ccgtgcgagc agccattgct ttcgatgagc aataattccc gcctgtgggg caatgtgaat
    36841 gtgttggcac gctgcggtaa cgacaaacga tatttacagg ttaatgtgca ggccacagga
    36901 aattatgtgg ttgccgcaat gcccattgtg cggggaggaa agctggaagc tggcaatgtc
    36961 aaactgaaac gcggacggct ggacaccttg ccaccgcgta cggtactgga tatcaatcaa
    37021 cttgttgatg ccgttagcct gcgcgatcta tcacccgatc aaccaatcca attaacccat
    37081 tttcgccagg catggcgggt aaaagcggga caacgcgtca acgtgatcgc cagcggtgat
    37141 gggtttagcg ccaacgcaga aggtcaggcg ctgaacaatg cagccgtcgc acagaatgca
    37201 cgggtgcgca tggtaactgg acaggtagtc agcggcgttg ttgatgcaga tgggaatatt
    37261 cttataaacc tgtaagctgt taaagattac ccgtcccttg ccgataaata agcaacatat
    37321 gataaaagcg ccctcaatga ggaataaacc atgagtattg atcgcacttc gcctctgaag
    37381 cccgtaagca ccgttcaacc gcgcgaaacc actgacgcgc cggtaacgaa cacccgggcg
    37441 gcaaaaacaa ccgcctccac cagcaccagt gtgacgttaa gcgacgcgca agcaaaactg
    37501 atgcaacccg gcagcagtga tatcaatctt gaacgtgtcg aagcgttaaa actggcgatt
    37561 cgtaacggtg aactaaaaat ggacaccggc aaaattgccg atgcgctgat caacgaagct
    37621 cagcaagact tgcagagtaa ctaatcgtat gacacgtctt gcagagatcc tcgaccagat
    37681 gtccgctgtg cttaacgatc tcaaaacggt aatggatcaa gagcagcaac atctctctat
    37741 ggggcagatc aacggcagcc agttgcaatg gattacagaa caaaaaagct ctctgctggc
    37801 gacgctggat tacctcgaac agttacgcag gaaagaaccc aattcagcaa atagcgttga
    37861 tattagtcaa cgctggcagg aaattactgt gaaaacgcag caactacgcc aactgaatca
    37921 acataacggc tggttactgg aaggacagat tgagcgcaat caacaggcgc tggaaatgtt
    37981 gaaaccgcat caggaaccga cgctatatgg agcgaacggt cagacctcaa caacccatcg
    38041 cggcagtaaa aagatttcga tctgaagatt atctccggcc tgcactgcag gccggaatac
    38101 attcttacac cgtccggcgg gcaaactctt taactttgaa gcccagtacc gccagtgcag
    38161 caaagtacgc agcaatcccc gccagcacga ccgccattaa acgcagtaag cgccagggca
    38221 tggtacccaa tgaccactcc ggcatgatat gtaacatacc taaaagcaca ccagacatca
    38281 ccagtaccgc caccaccaga cgcaacagaa acgccatcca gccaggttgc ggggtaaaga
    38341 ttttctgctt acgcaactgc cagtaaagca gcgaagcatt cagacacgcc gccagaccaa
    38401 tagaaagtga cagcccggca tgtttcaacg gaccaataaa cgccaggttc atcaattgcg
    38461 ttaaaatcag cgtaacgatg gcaattttca ctggcgtttt aatgtcctgg cgggaataaa
    38521 agccaggagc caacactttc actacaatca ggccaatcaa acccaccgag taggcaatta
    38581 acgcccgctg ggtcatcagc gcatcaaagg cggtaaattt accgtactgg aacagcgaaa
    38641 cggttaacgg accggaaaga atgcccaacg caaccgcact cggcagcgcc aacaggaaac
    38701 aaagacgcag cccccagtcc atcaaacggt tgtattcatc atgattgcca ctggcaaaac
    38761 ttttcgacag cgacggtagc aaaatggtgc caagcgccac gcccagcaca ccggacggaa
    38821 actccattaa gcggtcggcg taatacatcc acgacaccga accggaagca agaaacgagg
    38881 caaaaatggt gttgatgatt aaggagatct ggctcacaga gacgccaagg attgccggtc
    38941 ccatctgttt caccacacgc attgctccgg catcgtggaa gttaatgcgt ggcaaaacca
    39001 gcatgccgat cttcttcaga tgcggtagct gatacaccag ctgcaggatg ccgccgaccg
    39061 taacagccca cgccagcgcc agcaccggtg gattaaagta cggcgcggca aacagcgcaa
    39121 aaccaatcat gctgatattg agcaatgtcg gggcaaacgc cggaattgag aagcggttcc
    39181 aggtattcag aatcgctccc accagcgacg ccagggagat cagcaagata tagggaaagg
    39241 ttatctttag tagctggctg gtcagggcaa atttgtcggc agtgtcagcg aagccaggcg
    39301 cggtcaccat gatcacccac ggtgcagcga gcatgccagc gaccgtcaca accgccagcg
    39361 caagtgtcag caggccagaa acataagaga caaagacccg cgtggcgtct tcaccctgct
    39421 tacttttata ttccgccaga atcggtacaa atgcctggga aaatgcccct tcggcaaaga
    39481 tacggcgtaa caagttagga agtttaaaag cgacgaaaaa ggcgtcggtt gccatccctg
    39541 cgccaaagat tctggcgaca attgcgtcac gtgcgaagcc aagcacacgc gaaaacatgg
    39601 tcatcgagct gacggcggcc agcgatttta ataaattcat cggtgttcta atccagaccc
    39661 acgggcaaaa cgcctgcaag gcaggcgcta aaagaggcga atagtctacc tggattatgg
    39721 tgaattgcta ccgccagatg ttacagggtt tattcactca tcgcatcgcg ccagatcttg
    39781 tcaacgatac gttgcgcccg cactgcctgt tcgccggcgg tttgcggagc tgtctgattt
    39841 tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc cacgttgctc aagcgtactc
    39901 tgccaaccag gaatcggttt atgcactacg ccctgcccgc gctcctcacg ccattcgcgc
    39961 atatccgtaa tgtcgatgag cgcaccatca gtcacggcct gcacggtttc acgctgactt
    40021 ccggcacgac gatgcataca ggtggtgatc tgcaaaggac cagccgaaaa atggtgctcg
    40081 gcaaacagca tttcgccagc gtcgttagtc agtaacgtac cgccatccag agaggctttg
    40141 ccacctgaca accacagcgc ggtatccacc acatgcagat aatcatccag caacgtgaaa
    40201 taaagatcgt gtggcccgac gctattggta cgatgtttat ccattcttag cgaggacgcg
    40261 gtggcgagtt gcgtttttaa ctcaccgtag agtggtgcga aacgacggtt aaaaccgacc
    40321 atcaacgtca gttttttccg cgccgccagt tccaccagcc gttcagcatc gcgcagattt
    40381 tctgccagcg gtttatcgac acagacatgt acccccgcat tgagtaacgt actgaccacg
    40441 tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat cgcaactggc ggcaaggctg
    40501 gataacgaat cagcataagg aatgcgccag ctgtcacaaa ttagcaaggc tttggcgcgc
    40561 gtaggcgacc acgcaccttg taacgtccag tcagacgctg ccgccagcac cggtaaccac
    40621 gctttttgcg cgatgccgcc taaccccact acgccgatac gtaatttttt cactttaatc
    40681 tcccagatgg gccagcaacg aatcaagacg ctgtttcagt tctgccactt cgatttccag
    40741 ggcttcgacg cgggcctgta aatcaccgtc agctgcgttc gacatatccg ttaccgccgg
    40801 cggattctca acctcaccac tgaaaagatg catgtagcgg ctttcgcgtt tacccggttc
    40861 gcgggccaga cgcaccacaa aaggaccatc ttcgcgattc gccagttgtt ccagcgtcga
    40921 ctccacttcc gccatatcgc tgaattcata cattcgcgcg gcgcggctgc gcagttcccc
    40981 cggcgtctgg gcaccacgca ataacaacgt ggtgattaac gccacttctg cggcgctcag
    41041 tttcagatcg ccaaattctg aattacaaaa acgttgctca tatttggtga cccgattacc
    41101 aaaaccgctc accgtgcgta gataatgacg tttgaccaga ttatccagct gttcctgcac
    41161 ttcagattca ctcagattca taaccggttc acggttcgtt ttctgattac aggccgtgac
    41221 tacaccattg actgagagcg ggtattgctc cggcgtggtc acctgttttt ccagcaagca
    41281 accaatcacg cgagcttcca gggcagtaag ttgatatttc atgagattct ccttagcggc
    41341 cgggcgtcca gtctggggta gttaatgccg tcagtacgtg atcgcgccat tgtccatcaa
    41401 tcaacagata gtctttcgca tagccttctt tttcaaaacc cagtcgcgcc agtaaatcac
    41461 cgctgcgttt attgtgcggc atataattag ccataatgcg atgaatatgt tgggtgcgct
    41521 gcatataacg aatggctgcg ctcagagctt caaacatgag tcctttgccc tgccattttt
    41581 gcccaatcga ataaccgaga tagcaggcat gaaaagagcc gcgaacaaca ttggaaaaat
    41641 tggcaacgcc aataatctct ttttcgtccg ggtcgaataa gccaaagtag aaagctgaac
    41701 cttgtttatg aaattcgtta atcatcccta gcctggcctg ccagcctgat ggataacagt
    41761 ggctttcgtc gcgcactggc tcccagggct tgaggaaatg gcgattctct gcgtaataat
    41821 ccgccagacg ccaggcatca cgatcatgca ccagacgcac gaccagtcgg tctgtggtta
    41881 agcgcacttt tggcacgtta ctgcgatagc caaacattct atacctactc cttcccgtaa
    41941 cgtctttaat acgctgtaag tattactata cctgcgcaac cagaccctgt gaaaagtcca
    42001 gaatgccatt ttctgcatta ttcacatatt tgataaaaac tctctatcaa agcgacgttt
    42061 tgataaaaaa atattgtccc cgaagtgatg ggaaaagtct tagcggcaat gcagaataga
    42121 aagtgctcat cttttatttc ccgggagggg aaatgtcccg cgtgtcgcag gcgaggaacc
    42181 tgggtaaata tttcctgctc atcgataata tgctggtcgt gctggggttc tttgttgtct
    42241 tcccgctgat ctctatccgc ttcgttgatc aaatgggctg ggccgccgtc atggtcggta
    42301 ttgccctcgg tctacgccag tttattcagc aaggtctggg tattttcggc ggcgcaattg
    42361 ccgaccgctt tggtgccaaa ccgatgattg tcaccggtat gctgatgcgt gccgccggat
    42421 tcgccacaat gggtatcgcc cacgaaccgt ggctactgtg gttttcatgc ctgctttcgg
    42481 gactcggcgg cacgttgttt gatcctccgc gttcggcgct ggtggtgaaa ttaatccgtc
    42541 cacagcagcg tggtcgtttt ttctcgctgt tgatgatgca ggacagtgcc ggtgcggtca
    42601 ttggcgcatt gttggggagc tggctgttgc aatacgactt tcgcctggtc tgcgccacag
    42661 gggcagtttt gtttgtgctg tgtgcagcgt tcaatgcgtg gttgttaccg gcatggaaac
    42721 tctccaccgt gcgcacgccc gttcgcgaag gcatgacccg cgtgatgcgc gacaagcgtt
    42781 ttgtcaccta tgtcctgacg ctggcgggtt actacatgct ggctgtacaa gtgatgctga
    42841 tgctgccaat tatggtcaac gacgtggcgg gcgcgccctc tgccgttaaa tggatgtatg
    42901 ccattgaagc gtgtctgtcg ttaacgttgc tctaccctat cgcccgctgg agtgaaaagc
    42961 attttcgtct ggaacaccgg ttaatggctg ggctgttgat aatgtcatta agcatgatgc
    43021 cagtaggcat ggtcagcggc ctgcaacaac ttttcaccct gatttgtctg ttttatatcg
    43081 ggtcgatcat tgccgagcct gcgcgtgaaa ccttaagtgc ttcgctggca gacgcaagag
    43141 ctcgcggcag ctatatgggg tttagccgtc tgggtctggc gattggcggc gctattggtt
    43201 atatcggtgg cggctggctg tttgatctgg gcaaatcggc gcaccagcca gagcttccgt
    43261 ggatgatgct gggcattatt ggcatcttca ctttccttgc gctgggttgg cagtttagcc
    43321 agaaacgcgc cgcgcgtcgt ttgcttgaac gcgacgcctg atttgatact cccccctgac
    43381 tcctccatac tgaaagtgaa ctgatagcca aatggaggag tcacgtgaag ctatacattt
    43441 acgatcactg cccttactgc ctcaaagccc gcatgatttt cggcctgaaa aatatccccg
    43501 tcgaattaca tgttctgctc aacgacgacg cagaaacacc cacccggatg gtcggtcaaa
    43561 aacaggttcc cattctgcaa aaagatgaca gccgctatat gccagaaagc atggacatcg
    43621 ttcactatgt cgataaactc gacggcaaac cgttactgac cggcaaacgt tcccctgcca
    43681 ttgaagagtg gctgcgcaag gtcaatggct acgccaacaa actgctgttg ccgcgttttg
    43741 ccaaatcggc atttgatgag ttttctactc ctgccgcgcg caaatatttc gtcgacaaga
    43801 aagaggccag cgcgggtaat tttgccgacc tgctggccca ctctgacggt ctgattaaga
    43861 atatcagcga tgatttacgt gcgctggaca aactgatcgt caaaccgaac gccgtgaatg
    43921 gcgaactttc ggaagatgat attcagctat ttccgctcct gcgtaatctg acgctagtag
    43981 ccggaatcaa ctggccaagc cgcgttgctg attaccgcga taatatggcg aaacagacac
    44041 aaatcaattt gttatcatca atggcgattt aatcctgacc cgccgaggca gcctgtattc
    44101 gcgggttgcc gccgttttca gttttttctg ctggcgcgtt tatcgctggt gacacatgct
    44161 gcgttgtgtc aggatattga cgcagatttc atgtcgagga ctaccatgaa caagttttta
    44221 tttgccgctg cattgatcgt cagtggcctg ctcgttggct gtaatcaact cacccaatac
    44281 accatcaccg aacaagaaat taaccagtcg cttgcgaaac ataataattt ctcaaaagat
    44341 atcggtttac ccggcgtggc tgacgcccat attgttctga caaacctgac cagccaaatt
    44401 ggtcgcgaag agccgaataa ggttacccta accggagacg ccaatctgga catgaactcc
    44461 ctgttcggta gccagaaagc gaccatgaaa ctgaagctga aagcgctgcc cgtgtttgat
    44521 aaagaaaaag gcgcgatctt cctgaaagag atggaagtgg tcgatgcgac ggtacaaccg
    44581 ggaaaaatgc aaacggtgat gcaaacgttg cttccctatt taaaccaggc attacgcaat
    44641 tactttaacc agcaacctgc ttacgtcctg cgcgaagatg gcagccaggg cgaagcaatg
    44701 gcgaagaaac tggcgaaagg cattgaagtg aagccaggcg aaattgtcat tccgtttact
    44761 gattaatcac gagggcgcat tcgcgccctt tatttttcgt gcaaaggaaa acgtttccgc
    44821 ttatcctttg tgtccggcaa aaacatccct tcagccggag catagagatt aatgactgca
    44881 ccatcccagg tattaaagat ccgctgccca gacgactggc accttcacct ccgcgatggc
    44941 gacatgttaa aaactgtcgt gccgtatacc agcgaaattt atggacgggc tatcgtaatg
    45001 cccaatctgg ctccgcccgt gaccaccgtt gaggctgccg tggcgtatcg ccagcgcatt
    45061 cttgacgccg tacctgccgg gcacgatttc accccgctga tgacctgtta tttaacagat
    45121 tcgctggatc ctaatgagct ggagcgcgga tttaacgaag gcgtgttcac cgctgcaaaa
    45181 ctttatccgg caaacgcaac cactaactcc agccacggcg ttacgtcagt tgacgcaatc
    45241 atgccggtac ttgagcgcat ggaaaaaatc ggtatgccgc tactggtgca tggtgaagta
    45301 acacatgcag atatcgacat ttttgatcgt gaagcgcgct ttatagaaag cgtgatggaa
    45361 ccgctgcgcc agcgcctgac tgcactgaaa gtcgtttttg agcacatcac caccaaagac
    45421 gctgccgact atgtccgtga cggaaatgaa cggctggctg ccaccatcac tccgcagcat
    45481 ctgatgttta accgcaacca tatgctggtt ggtggcgtgc gtccgcacct gtattgtcta
    45541 cccatcctca aacgcaatat ccaccaacag gcattgcgtg aactggtcgc cagcggtttt
    45601 aatcgtgtat tcctcggaac ggattctgcg ccacatgcac gtcatcgcaa agagagcagc
    45661 tgcggctgcg cgggctgttt caacgcccca accgcgctgg gcagttacgc taccgtcttt
    45721 gaagagatga atgctttgca gcactttgaa gcattctgtt ctgtaaacgg cccgcagttc
    45781 tatggcttgc cggtcaacga cacattcatc gaactggtac gtgaagagca acaggttgct
    45841 gaaagcatcg cactgactga tgacacgctg gtgccattcc tcgccggaga aacggtacgc
    45901 tggtccgtta aacaataaaa atcgtagctt cctgttgtca ttaggttatt ttacctgtat
    45961 aaataaccag tatattcaac agggggctat tatgcgaatt gaagtcacca tagcgaaaac
    46021 ttctccattg ccagctgggg ctattgacgc cctggctggc gaactttccc gccgtattca
    46081 gtatgcgttt cctgataatg aaggccacgt atcggtacgt tatgccgcag caaataattt
    46141 atcggttatt ggcgcgacaa aagaagataa acagcgcatt agcgaaattc tccaggaaac
    46201 gtgggaaagc gccgatgact ggtttgtcag cgaataatat gcagtgattt ttgtttttgc
    46261 cgggtcgccc cggctttttt attttacttg cgaattgtga gtacaaaatt tctacacttc
    46321 ctcaaaataa ttgcacaata tgctaaaaaa atatgaatga catgctgttt tattgttcat
    46381 ataaatctta aaagcagaaa tttatctgct ccccccttta aaaattcggt gaataccctt
    46441 acttattggt atactgaagt tgcttcagat aaaaacgcat tgaacctcga ataacgttgt
    46501 ctagtaacac gaattagggg gccatgatgg aaaaaaataa tgaagtcatt cagactcatc
    46561 cgctcgtagg gtgggacatc agcaccgttg atagctatga tgcgctgatg ttgcgtttgc
    46621 actaccagac cccaaataag tccgagcagg aagggactga agttggtcag acgctctggt
    46681 taaccactga tgttgccaga caatttattt cgatattaga agcaggaatc gccaaaattg
    46741 aatccggtga tttccaggta aacgagtatc ggcgtcatta acatgacatg accatccacc
    46801 caaaaggcac ctcaccggtg cctttaccat ttctgggttg tatatctgca ttcagttaat
    46861 tactctgctt aaaccatgag agcagagaga aaaaaatgaa atacgatctc atcattattg
    46921 gcagcggttc cgtaggcgct gccgccgggt attatgcaac ccgcgccggt ttaaacgtgc
    46981 taatgaccga cgcccatatg ccaccgcatc aacacggcag ccaccacggc gatacgcgat
    47041 taattcgcca tgcttatggt gaaggcgaaa agtatgtccc gctggtcctc cgcgcgcaaa
    47101 tgctgtggga tgaactttcc cgccacaacg aagatgatcc catttttgta cgctctggtg
    47161 tcattaacct cggtccggct gactccgcat ttctcgccaa cgtcgcccac agcgccgaac
    47221 agtggcaact caacgttgaa aagctcgacg cgcaagggat tatggcccgc tggccagaaa
    47281 tacgcgtccc ggacaactac atcggtttat ttgaaactga ttccggtttt ttgcgcagcg
    47341 aactggcgat taaaacctgg atccaactgg cgaaggaagc gggctgtgcg cagctgttca
    47401 actgcccggt caccgcaatt cgtcatgacg atgatggcgt aactattgaa acggctgacg
    47461 gtgagtatca ggcgaaaaaa gcgattgtct gcgcgggaac atgggtaaaa gacctactcc
    47521 cggagctgcc tgtccagccc gtacgcaaag tatttgcctg gtatcaggcc gatggtcgct
    47581 atagcgtgaa gaataaattc ccggcgttta ccggtgaact gcccaatggc gatcaatatt
    47641 atggttttcc ggcagaaaac gacgcgttga agattggcaa acataacgga ggccaggtta
    47701 tccattcagc ggatgaacgt gttccgtttg cggaagtggt cagcgatggt tcggaagcct
    47761 tcccgttctt gcgcaatgtg ttgccgggta tcggttgctg cctgtacggc gctgcctgca
    47821 cctatgataa ttcgcctgac gaagatttta ttatcgatac cctacccggc cacgataata
    47881 cactgctcat taccggcctg agtgggcacg gttttaaatt tgcgtcagtt ttaggggaaa
    47941 tagctgccga ttttgcgcaa gacaaaaaaa gcgattttga tttgacacca ttcaggcttt
    48001 cccgcttcca ataatcatca ttacggcctc cagaatgagg ccgtttttac aggagtattt
    48061 aatgcgtcgg ttggtgaact atctcattaa taatattcgc gagcatctga tgctttatct
    48121 ttttctctgg ggactattag caattatgga cctaatctac gtgttttatt tttagttttc
    48181 cgcactgaca taaactgaca aatatttaat ctccctttca tttagcgtga aaaattgaat
    48241 gagattcttc ctgcctctac agcactcact agaccttcca gatgcttaac ccactaaact
    48301 tatgaagatt ctttcattat taattcaaaa ttattgaaat aaaaataaat atcatttaat
    48361 ttgttatttt tgtgttgcaa ttcattctcc gacacgccat tttctttctc tcgcaaattc
    48421 gttttggatg tacttatgtc attcacaaat acccctgaac gctatggtgt tatatctgcg
    48481 gccttacatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg tctgtggatg
    48541 gtcacgctca gttattacga tggctggtat cacaaagcac ccgaactgca taaaagcatc
    48601 ggtattttgt taatgatggg gctggttatt cgcgttctgt ggcgtgtcat atctccaccg
    48661 ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg cagcaaaagc tggacatctc
    48721 gcattgtacc tattgctgtt cgccatcggc atcagcggct atctgatctc aactgccgat
    48781 ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgaccctcgc cgacgctggc
    48841 gcacaggctg actttgccgg tgccctgcat ttttggctcg cgtggagcgt cgtagtactg
    48901 tccgttatgc acggatttat ggccctgaag catcatttca tcgataaaga cgacactctg
    48961 aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa taaaagcctg
    49021 cttggtttaa ccttcgcttc cctgatgttc tctgccggtt cagcggttgc cgccgattac
    49081 aaaattgaca aagaaggtca gcacgccttt gttaacttcc gcatccagca tcttggctat
    49141 agctggttat atggcacctt taaagatttc gacggtactt ttacctttga tgaaaaaaat
    49201 ccggctgccg ataaagtgaa tgtgacaatt aacacaacca gcgtcgatac taatcacgcc
    49261 gaacgcgata aacatcttcg cagtgcagat ttcctcaata ctgcaaaata tccacaggca
    49321 acattcacct ccaccagcgt gaagaaagac ggtgacgaac tggatattac cggcgatctg
    49381 acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga aattaattgg tcaaggcgac
    49441 gacccgtggg gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa gctcaaggac
    49501 ttcaatatca aaacagattt aggtccagct tctcaggaag tggatctgat tatttcagtg
    49561 gaaggcgtac agcagaagta agagtgaaat gttgccggat ggcacgcatc cggcaatgat
    49621 atttattctg ttggatcagg aatgcccagt gttgtattca gacgtccacg agacttatta
    49681 aagatcttat tgccattttc acgtcctgcc cgacggcgtc gctgttcttc aggcggtaac
    49741 gcgctttctt cgcagcaaat ctcactacaa caacctttgt atttttccgc acatactgga
    49801 cactgaataa aaagcaggtg gcagccatca tttttacagt tggtatggct gtcgcacggc
    49861 gcaccgcact ggtggcaatg cgcgataatc tcatcagata tacgttcgcc catccgctcg
    49921 tcaaaaacaa aatttttgcc aataaaacgt accggtaaac cctgctcgcg cgccttgcgg
    49981 gcgtattcaa taattccgcc ctcgatatgc cagactttat tgaatccgtt atgtttcatc
    50041 caggcactgg ccttttcaca acgaatgccg ccggtgcagt acataacgat ttttttatct
    50101 ttatgtgcct gcatcatctc gactgctttt ggcagctgct cacggaaggt atctgccgga
    50161 atttccagcg cgttttcaaa gtgccccact tcatactcat agtggttacg catgtcgata
    50221 aacaatgcat cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag atactcaccc
    50281 acattgctgg catcaaagtg aggatcgtca ataccgtcgg caacgatacg atcgcggacc
    50341 ttcatgcgca gtacccagaa ggatttcccg tcatcatcca acgcgatatt caggcgtaag
    50401 ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa atgtttcaac attgctcgca
    50461 ggtacgctga tttgcgcgtt aatgccctca tgcgccagat acactcgccc aaaaacattc
    50521 agcgcggtaa acagttgata taaagcgtca cgggtcacct taggatcggc aatgtggaaa
    50581 tacttataaa acgaaatggt ggttcgcggt tcgctctcag ccaacatttt ggcttttaac
    50641 gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt acggttcctg cgagatggga
    50701 aagtaaaaat ccgcggcatg atatagcaat tatcgataat taacatccac acattttacg
    50761 ctacatttta gcattaaaaa ttatttgtta tttacaagcg cggcaatttc gcccagtctt
    50821 cagtcacaaa ttttggttgc ggacgaaaaa atgcgacaat acatacaatt gcccgtatag
    50881 gttgaaaaac aggattgata tgacgaatct acccaagttc tccaccgcac tgcttcatcc
    50941 gcgttattgg ttaacctggt tgggtattgg cgtactttgg ttggtcgtgc aattgcccta
    51001 cccggttatc taccgcctcg gttgtggatt aggaaaactg gcgttacgtt ttatgaaacg
    51061 acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc ccggaaatga gcgaacaaga
    51121 acgccgtaaa atggtggtga agaatttcga atccgttggc atgggcctga tggaaaccgg
    51181 catggcgtgg ttctggccgg accgccgaat cgcccgctgg acggaagtga tcggcatgga
    51241 acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca tccattttct
    51301 gacactggaa ctgggtgcgc ggcagtttgg tatgcaggaa ccggggattg gcgtttaccg
    51361 cccgaacgat aatccactga ttgactggct acaaacctgg ggccgtttgc gctcaaataa
    51421 atcgatgctc gaccgcaaag atttaaaagg catgattaaa gccctgaaaa aaggcgaagt
    51481 ggtctggtac gcaccggatc atgattacgg cccgcgctca agcgttttcg tcccgttgtt
    51541 tgccgttgag caggctgcga ccacgaccgg aacctggatg ctggcacgga tgtccggcgc
    51601 atgtctggtg cccttcgttc cacgccgtaa gccagatggc aaagggtatc agttgattat
    51661 actgccgcca gagtgttctc cgccgctgga tgatgccgaa actaccgccg cgtggatgaa
    51721 caaagtggtc gaaaaatgta tcatgatggc accagagcag tatatgtggt tacaccgtcg
    51781 ctttaaaaca cgcccggaag gcgttccttc acgctattaa atcttccatg ctggatgctt
    51841 cagaatggca tccggcatta ccacagaaaa tccccctgat ttagcgataa aagctctctg
    51901 gattgcgccc cctggaagtc gggcgcataa ttagtgtgct tatcttttct tcttatgttc
    51961 accgcgcctg gcgcaccaac agcggattgc tatgtcaccc tgtgaaaatg acacccctat
    52021 aaactggaaa cgaaacctga tcgttgcctg gctaggctgt tttcttaccg gtgccgcctt
    52081 cagcctggta atgcccttct tacccctcta cgttgagcag cttggcgtta ccggccactc
    52141 cgccctgaat atgtggtccg gtattgtctt cagcattaca tttttatttt cggccatcgc
    52201 ctcaccgttt tggggtggac tcgccgaccg taaaggccga aaaatcatgc tattacgctc
    52261 tgctctcggc atgggcatcg tgatggtgtt gatgggactg gcacaaaata tctggcagtt
    52321 tttgatcctg cgggcgcttc ttggtttact tggcggattt gtccccaacg ctaatgctct
    52381 tatcgccaca caagtaccgc gtaataaaag cggctgggcg ctgggtacgc tctccacagg
    52441 cggcgttagc ggcgcgttgc tcggcccaat ggctggcggt ttgctcgccg atagctacgg
    52501 cttacgtccg gtattcttta ttaccgccag tgtgctcata ctctgctttt tcgtcaccct
    52561 gttttgcatc agagaaaaat tccagccggt cagcaaaaaa gagatgctgc atatgcggga
    52621 agtggtgaca tcacttaaaa acccgaaact ggtactcagc ctgtttgtca ccacgttaat
    52681 catccaggtg gcgacgggct caattgcccc cattctgacg ctgtatgtcc gcgaactggc
    52741 gggtaacgtc agtaacgtcg cttttatcag tggcatgatc gcctcggtgc caggcgtggc
    52801 ggctctgcta agtgcaccac gactcggcaa acttggcgat cgaatcggac ccgaaaagat
    52861 cctgattacg gcgctgatct tttctgtact gctgttgatc ccaatgtctt acgttcagac
    52921 gccattacaa cttgggattt tacgtttttt gctcggtgcc gccgatggtg cactactccc
    52981 cgccgtacag acactgttgg tttacaactc gagcaaccaa atcgccgggc gtatcttcag
    53041 ctataaccag tcgtttcgtg atattggaaa cgttaccgga ccattgatgg gagccgcgat
    53101 ttcagcgaac tacggtttca gagcggtatt tctcgtcacc gctggcgtag tgttattcaa
    53161 cgcagtctat tcatggaaca gtctacgtcg tcgtcgaata ccccaggtat cgaactgatt
    53221 tttcgccttt catacttgca aaagcggaga atcagctatc cttttccctg aaacctcatc
    53281 aactcaaagg gagaatcgtg atgaccatgt acgcaacgct tgaagaagcc attgacgctg
    53341 cacgcgaaga atttcttgca gacaaccccg gcatcgacgc cgaaaacgcg aatgtgcaac
    53401 agttcaatgc ccaaaaatac gttttgcagg acggcgacat catgtggcaa gttgagtttt
    53461 ttgccgacga aggggaagaa ggtgaatgtt tacccatgct tagcggtgaa gctgcgcaaa
    53521 gtgtctttga cggcgactat gatgagatag agatccgcca ggagtggcag gaagagaata
    53581 cattacatga atgggacgag ggggaatttc agcttgagcc accgctggat accgaggaag
    53641 gacgcgcagc agctgatgag tgggatgaac gttaatcact catacgggcc atgatgaatg
    53701 tcgatcggca gcagtaaagt atcgaaaacc agggagaatg gcagatcaag gatcgtgacg
    53761 taacgccagg cggagtcacg cacgtcccat tgcacaccgg gataatattg gttgccatgc
    53821 ccctgcccct gtatagtgcg actaatgatg ctgccacagc cactcagcaa ggtcaccatt
    53881 atgctcacca caattaaacg catttgatac ctcttccgta aaaaataccg gcataacgcc
    53941 ggtattttta ttggctcgtt gtttttgccg gatgcggcgt gaacgcttta cccgacctac
    54001 aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc gtagcgcatc
    54061 aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag tggattgagc
    54121 tttatccctt cgtaataact cacccatgaa gaatatctct ccggggaatt ccagacgcgg
    54181 aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg atcgcgattc
    54241 agcttctctg gcgtctcgtt cagcgcctgt tcaacgtggc ggtcacgggc gatttccagc
    54301 accttactgg cgcggtgacg cgcggtcgcc attgcggttg ccagagcgtt gaatgacggg
    54361 ttaaacaccg cgtgcataaa accatcatca agggagcgtt gacgattcat ctcaaggaac
    54421 cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag gaacagtttc
    54481 cagcgtttgg ttcgcagacc aacggtggca cggctggaaa tcaccgaaac aaacggtgac
    54541 aggatcaacg agaagacaat cggtgccagc cagaacagaa aacgcagatc caaccacgcc
    54601 ataccaactg cccacactaa ccctaacagc agctgtgagc cgtggcgttt gaacgcttcc
    54661 ccccaggaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc ccagccaagg
    54721 aacgcgctga cgacgaagac cgtatggaac agcatgcgta ccggagccag cagcacggaa
    54781 aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta ttctttcgtt
    54841 cctttgcacc agataagcaa aatgctcaat agcttcggca ggaacaacag caccatggtc
    54901 gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccataccgg gaacaactgc
    54961 cgtggttgca ggaagtattg cggttcggtc aacgcatgta ctacctgcaa tgcagtagag
    55021 agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc cgtcaggaac
    55081 accgcacggt gaaccgggtg catacccttc accaggaaca gacggaagtt catcaggtta
    55141 ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg caattcttca
    55201 taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg catcaacgcc
    55261 gcttccacga agtcatgtga caggattgaa ccggcaaaag aaccttcgcc cggcagcgga
    55321 gccagtgcac agtgctcgat aaacggtttc acgcggataa tcgcgttatg cccccagtag
    55381 tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg
    55441 gtcgcgaact gctgacagcg cgcatacagc gtatccatgc cggacgcttt cggcgacgac
    55501 tgaatgatcc cggcgttcgg gttggcttcc atcaggcgca ccaggccgca caaacaatca
    55561 ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct gccccaacga
    55621 cggcagaagt catcgatatt accgctttta cgcttcacgc gacggcggcg gcggcgatag
    55681 aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt ttgctctgcg
    55741 acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg tttggcattc
    55801 ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg gttcacgtct
    55861 tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca
    55921 actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag gaagcccatc
    55981 aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaagag gatcaggata
    56041 ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacacatc ctgaccaacc
    56101 atatccatag gattaatcag cgcccacccc tgataaggaa gaatggtctt catataccag
    56161 gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg gatggtaccg
    56221 acagtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc cagatagcgc
    56281 ggcgtcacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt ggtacgccac
    56341 gggtcgggaa acatcgagga gcgttttgct tctggcatcg cctttagctg atcgcgccct
    56401 tcgtcgtctt tgattaactg tccatcagca agtgaatctg gccaggcttg ttccagacgc
    56461 gcctttaccg agccttgcgg ggagtcatcc tcccgcgccc aggtgcggtg ttcggcatcc
    56521 agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ccgctttctc gcttgcggcg
    56581 atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact ggtagctcca
    56641 ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag tggttttctt
    56701 ggcatctttc actttcacac gcatcaccag acgccaacct ttggtaaccg ggttatagcg
    56761 caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg tgaccggggt
    56821 atcctctggc agttttttca tctcagcgcc ggtaaaatcg accacaaagg cgatagtacc
    56881 gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac gcgtttgttg
    56941 cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg tgatggtgta
    57001 tttaaagttc atctctttac ccggctccgg cagttgatcc ggcgtccagt aagcgacgat
    57061 gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc ctttgcccca
    57121 ctcccctttc ggagtcaccc atgcgcttgg acgaagatcg taacgatcat cgagatcttc
    57181 aaagcgggag aaatcacgac cacgctgcaa tagaccgaag ccttgcgggt tttccatcga
    57241 gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc attcgccatt
    57301 accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt tatttgcagg
    57361 cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc ccagtttgcc
    57421 gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac gtcctggcat
    57481 aactacgaat ttgtaagcac ctgtcgcgcg cggcgagtca agcaatgcat aaatggttaa
    57541 acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg gaaattcttc
    57601 acccgatggc aaggcggtat caattgccag gccgcgtgca gaaaggccat aaacctgacc
    57661 tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat cgtttttatc
    57721 tttgctgttg atcgggtaaa gcactttgaa accggcaaaa ccaaggtctt ttaccgtgtc
    57781 tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac
    57841 tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac cctgatggta
    57901 gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat gattaaactg
    57961 gatctgctga tagtccgcgt atttcatatc gcggaaaacg gagggcaagt tgcttttggg
    58021 cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat caatactgaa
    58081 agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc aacgcatttt
    58141 catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg tgcttatatc
    58201 gatccgatct attttaatgg ataacctgcc attccgacaa cagaatcccg gtatcgttca
    58261 gcacactggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc
    58321 taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta tcaaccgtct
    58381 tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa accttgaagc
    58441 ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg cataacccga
    58501 cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt acccgcgcaa
    58561 cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat cccttttcat
    58621 atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc gtcattgtgg
    58681 ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtgttttt cgttatatcc
    58741 gggtactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg gaaagtacgt
    58801 gtcgaacgtg tagggatccc gatgttaaca gccatcccct tactgacatt accgcaattt
    58861 attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc attgtatgac
    58921 aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact ggtgttagtg
    58981 gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt agaaaattcc
    59041 gataaaacga ataaaaaatt ctcgatggta aaattatcgg tgattttttt gtgcctcggc
    59101 atcggttatg cggtaataag aagaacgatt tttattgtgt atccacccat tctgagtaac
    59161 ggcatgttca attttattgt catgcaaacg ttattttatt tgccgttctt tatcctcggc
    59221 gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt
    59281 acccttgcag cagcattggc gtttgtcgct tatttactca accagcgcta tggcagtggc
    59341 gatgcctgga tgtatgaaac cgagtcggtg atcactatgg tcctcggtct gtggatggtg
    59401 aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg ggtgacttat
    59461 tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct gtttttcggc
    59521 gcatacatta caccacacat cacctccaac tggcttggtt ttctctgtgg cctgatattc
    59581 gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccatt attgaagttt
    59641 ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcacctgc ccgttaagcc
    59701 gcgtttacaa taaccattcc acgggcaata tcgacgccag tttgaccata acccgcttcc
    59761 agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga cgatcaaccc
    59821 agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac tggctctgaa
    59881 taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag cccatttcag
    59941 tattgagcaa cgttgaacgc ggatcgaaat tgaaagagcc gataaacacc gttttaccat
    60001 cgatactaaa ggttttagca tgcaggctgg cccctgaatt accggttatg ccgcgatcgt
    60061 gtaacgtact actttgttca cgcgtcggct tgagctcata taattccacc ccatagcgga
    60121 gcaatttttt gcgccagcgc gcgtatcctg catggacgac agcaacatcg ttagcggcaa
    60181 gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag agttgcgcca
    60241 cacctgcacg tgtcggtaca aaataggaag agataatatc gatgcgttca ctgggtgagc
    60301 ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg ccctcccctt
    60361 tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac gttccatcaa
    60421 ccagatgatt tataaacgga ctggattcca ttttgcgtaa gtaacgatgc gtcatgacat
    60481 cgttatgcca ggaggcgggt aactcgatgc gatctgccat ttcaccttcc gggacatcca
    60541 gcacctgctg taagggggaa accgatttgc aataccaata gcgggcgaaa tcatcggcaa
    60601 cgtcctctac cacgggtcct attgccatga catctaaatc cgaaaaaagt ggctcttccc
    60661 ctgcaccaaa ataggcatca ccaatatttc gtcctcccac cagcgtcacc acgccatcga
    60721 cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg atataaccaa
    60781 gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg cgtggatggc
    60841 tgtcaagcaa gcgtaaaatg ttatcaagtc cgggcgtatt gttgtcgtcc agcaacaaac
    60901 gtacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac cgacccgaca
    60961 tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt tcagccagac
    61021 gataccgggc ggcaaacgca tccagacttt tctccagagg aaaaagtcca cactgaccgg
    61081 gatgttgcga acacagtggc agcaccgcgc tcgccagccg aggcaaatca ttcatctcct
    61141 tgttgagtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca gacaaagtat
    61201 acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggtttttac tgcgatttca
    61261 gccgctgccg cacgagggta accataaaca ccagtactaa ttgcaggaaa agccactgac
    61321 gtatagctgt ttgccgccac cagtcgcagg ctattgagat aggcatcctg caaaagctgg
    61381 tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgcactac ggctttagcg
    61441 ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc ctgctgttgc
    61501 ctgactttta aacaggcatc cagcagcgcc ggacccgctg cgcgatgaat ggccccatcg
    61561 acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc aacggccagt
    61621 ttggtaatat cacccagcac aacatgaata cgcgttttca taacgcctcc ttacaccttc
    61681 ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc tcagcaaggt
    61741 gagttgttca gaaagttagt tatattcgat gttcattaca gccagctttt tctgtttatc
    61801 aagatactgc acttttactg aggctatttg cgcgttttta acattgccgc tcattaacgc
    61861 tttgctggaa taggttttac gcggaacaga accattctgt gggcagttca tttctacggg
    61921 tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa aatggatcac
    61981 gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac tgccgagcat
    62041 aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta gtcatccttg
    62101 agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt ctctccaggt
    62161 tcatcttatg ctcgatattt caacaaatta agacttttct gaagatggcg gccattgttg
    62221 tgataaatga agtgactgtc catcagaaac agtaacaact attttcaccc gatcgtccgg
    62281 ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca atgaaagggt
    62341 cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca atatttgtac
    62401 tctgcacaga caagattgag taagagtgac ttcaggaata atggtgtaca tatccccttg
    62461 ctgggtcgta ttaaaggtta tctgactgga aagtgccgca aggagtaata acgcattcat
    62521 attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tttaatacag aggatgtatt
    62581 agtactgatg agcggtcgcg ttgttaccaa agccaacctg agtgacgtta acgctggagt
    62641 tagatgcagt ctggtcaaca gcagcaccgt taccgccacc gaactgttta acagtcatag
    62701 tagaatcttt accattccac tgatcaagag tagcgctgtt gccgaaacca cgctgagtca
    62761 gatcgattga gctgtcatca gaaccttggc caacatctgc gccgttaccg ccgccgtgct
    62821 gggtaatggt cagatcagag ttacgggcgt cagcttgcag agcaagagca gagttaccgc
    62881 caccgtactg gtaaatattc agctctgaat tcggaccgct gttattaccg ccaccaccgt
    62941 ggttgccacc gccgccgccg tactgaggaa caacacctgc cagagcgcta ccggagaata
    63001 caattgctgc aattgctgct acttttaaaa gtttcatgta aaacccccat cggattgatt
    63061 taaaagtcga atggaaatta acgttgtgtc acgcgaatag ccatttgcga ctgtctctgc
    63121 actacaattg ccgttttttg agtaccatac tgtgtaatat ttgctttatt accagaacct
    63181 ttctggataa tcatcgcagt attaccataa gcaccttgcg aaatactggc atcgttggca
    63241 ctgcccgcct gatcaatata tgcaaggtta taatctcctg tctgatcaat ctttgcccgg
    63301 ttgctactac cttcttgcgc aacaaccgcc aaaagttttg agcctccctg ccgtaactga
    63361 gcactattat tagtcccagc ttgaccaatt atggctgcct gattaaatga agacttactc
    63421 aattcattta ccgcaaagtt atattctgaa ttagctaaat cataacctgc tgcggctgca
    63481 atcccaggcg cacccagtat tgttaacatc ataaataaca atttgttttt catgttgtca
    63541 ccctggacct ggtcgtacat ttaagaaatt aaatcatttc aacttggttg ttaacgcaac
    63601 ctgtattttg ttaacgctgc gttacgatgg aaagtatgtc tgcggaaata tttttaataa
    63661 ctcacccgcg cgttgtattt ttcttttttt gttcatacca aagtatt
//
LOCUS       DAZKHK010000010        62949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKHK010000010 DAZKHK010000000
VERSION     DAZKHK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.010"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..82
                     /locus_tag="ACHOS5_000658"
     CDS             <1..82
                     /locus_tag="ACHOS5_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775538.1"
                     /translation="NFAGDCETAFALAVKFALAQLLQLLL"
     gene            complement(127..2172)
                     /gene="dcp"
                     /locus_tag="ACHOS5_000659"
     CDS             complement(127..2172)
                     /gene="dcp"
                     /locus_tag="ACHOS5_000659"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP9775539.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFKNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDVAQLAGMSEQEIAQAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRTIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            2309..3055
                     /gene="ydfG"
                     /locus_tag="ACHOS5_000660"
     CDS             2309..3055
                     /gene="ydfG"
                     /locus_tag="ACHOS5_000660"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP9775540.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3144..3830
                     /gene="rspR"
                     /locus_tag="ACHOS5_000661"
                     /gene_synonym="ydfH"
     CDS             3144..3830
                     /gene="rspR"
                     /locus_tag="ACHOS5_000661"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP9775541.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4007..4210
                     /gene="ydfZ"
                     /locus_tag="ACHOS5_000662"
     CDS             4007..4210
                     /gene="ydfZ"
                     /locus_tag="ACHOS5_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP9775542.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4246..5706)
                     /gene="ydfI"
                     /locus_tag="ACHOS5_000663"
     CDS             complement(4246..5706)
                     /gene="ydfI"
                     /locus_tag="ACHOS5_000663"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9775543.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTGRVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     MIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYVQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYTLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(5795..7162)
                     /gene="ydfJ"
                     /locus_tag="ACHOS5_000664"
     CDS             complement(5795..7162)
                     /gene="ydfJ"
                     /locus_tag="ACHOS5_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFP9775544.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPHVVRAAQ
                     PSRSL"
     gene            complement(7220..8239)
                     /gene="rspB"
                     /locus_tag="ACHOS5_000665"
     CDS             complement(7220..8239)
                     /gene="rspB"
                     /locus_tag="ACHOS5_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFP9775545.1"
                     /translation="MKSILIEKPNQLAIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(8251..9465)
                     /gene="rspA"
                     /locus_tag="ACHOS5_000666"
     CDS             complement(8251..9465)
                     /gene="rspA"
                     /locus_tag="ACHOS5_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP9775546.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(9671..9997)
                     /gene="ynfA"
                     /locus_tag="ACHOS5_000667"
     CDS             complement(9671..9997)
                     /gene="ynfA"
                     /locus_tag="ACHOS5_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP9775547.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            10132..10473
                     /gene="ynfB"
                     /locus_tag="ACHOS5_000668"
     CDS             10132..10473
                     /gene="ynfB"
                     /locus_tag="ACHOS5_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP9775548.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            10508..11068
                     /gene="speG"
                     /locus_tag="ACHOS5_000669"
     CDS             10508..11068
                     /gene="speG"
                     /locus_tag="ACHOS5_000669"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP9775549.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(11071..11781)
                     /gene="ynfC"
                     /locus_tag="ACHOS5_000670"
     CDS             complement(11071..11781)
                     /gene="ynfC"
                     /locus_tag="ACHOS5_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP9775550.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            11889..12194
                     /gene="ynfD"
                     /locus_tag="ACHOS5_000671"
     CDS             11889..12194
                     /gene="ynfD"
                     /locus_tag="ACHOS5_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP9775551.1"
                     /translation="MKLSTCCAALLLALASPTALAAPGSCERIQSDISQRIINNGVPE
                     NSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
     gene            12393..14819
                     /gene="ynfE"
                     /locus_tag="ACHOS5_000672"
     CDS             12393..14819
                     /gene="ynfE"
                     /locus_tag="ACHOS5_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP9775552.1"
                     /translation="MSKSKQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVKQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     SAFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            14880..17303
                     /gene="ynfF"
                     /locus_tag="ACHOS5_000673"
     CDS             14880..17303
                     /gene="ynfF"
                     /locus_tag="ACHOS5_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP9775553.1"
                     /translation="MKIHNTEALMSAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAQAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     TDSIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FRVDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            17314..17931
                     /gene="ynfG"
                     /locus_tag="ACHOS5_000674"
     CDS             17314..17931
                     /gene="ynfG"
                     /locus_tag="ACHOS5_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP9775554.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            17933..18787
                     /gene="ynfH"
                     /locus_tag="ACHOS5_000675"
     CDS             17933..18787
                     /gene="ynfH"
                     /locus_tag="ACHOS5_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP9775555.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYCLHM
                     TVGMAIAG"
     gene            18830..19444
                     /gene="dmsD"
                     /locus_tag="ACHOS5_000676"
     CDS             18830..19444
                     /gene="dmsD"
                     /locus_tag="ACHOS5_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP9775556.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDSWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            19604..20896
                     /gene="clcB"
                     /locus_tag="ACHOS5_000677"
     CDS             19604..20896
                     /gene="clcB"
                     /locus_tag="ACHOS5_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP9775557.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALATSCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLISNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LTVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(20849..21544)
                     /gene="bioD"
                     /locus_tag="ACHOS5_000678"
     CDS             complement(20849..21544)
                     /gene="bioD"
                     /locus_tag="ACHOS5_000678"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9775558.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(21669..22889)
                     /gene="mlc"
                     /locus_tag="ACHOS5_000679"
                     /gene_synonym="dgsA"
     CDS             complement(21669..22889)
                     /gene="mlc"
                     /locus_tag="ACHOS5_000679"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP9775559.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLERIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(23024..23917)
                     /gene="ynfL"
                     /locus_tag="ACHOS5_000680"
     CDS             complement(23024..23917)
                     /gene="ynfL"
                     /locus_tag="ACHOS5_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9775560.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            24024..25277
                     /gene="ynfM"
                     /locus_tag="ACHOS5_000681"
     CDS             24024..25277
                     /gene="ynfM"
                     /locus_tag="ACHOS5_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9775561.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            complement(25327..25446)
                     /locus_tag="ACHOS5_000682"
     CDS             complement(25327..25446)
                     /locus_tag="ACHOS5_000682"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP9775562.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            25702..26010
                     /gene="asr"
                     /locus_tag="ACHOS5_000683"
     CDS             25702..26010
                     /gene="asr"
                     /locus_tag="ACHOS5_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP9775563.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            26103..26186
                     /locus_tag="ACHOS5_000684"
     CDS             26103..26186
                     /locus_tag="ACHOS5_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775564.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            26286..27107
                     /gene="ydgD"
                     /locus_tag="ACHOS5_000685"
     CDS             26286..27107
                     /gene="ydgD"
                     /locus_tag="ACHOS5_000685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP9775565.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(27146..27475)
                     /gene="mdtI"
                     /locus_tag="ACHOS5_000686"
     CDS             complement(27146..27475)
                     /gene="mdtI"
                     /locus_tag="ACHOS5_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP9775566.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRCKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(27462..27827)
                     /gene="mdtJ"
                     /locus_tag="ACHOS5_000687"
     CDS             complement(27462..27827)
                     /gene="mdtJ"
                     /locus_tag="ACHOS5_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP9775567.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            28239..29273
                     /gene="tqsA"
                     /locus_tag="ACHOS5_000688"
     CDS             28239..29273
                     /gene="tqsA"
                     /locus_tag="ACHOS5_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP9775568.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRIGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(29298..30686)
                     /gene="pntB"
                     /locus_tag="ACHOS5_000689"
     CDS             complement(29298..30686)
                     /gene="pntB"
                     /locus_tag="ACHOS5_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP9775569.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(30697..32229)
                     /gene="pntA"
                     /locus_tag="ACHOS5_000690"
     CDS             complement(30697..32229)
                     /gene="pntA"
                     /locus_tag="ACHOS5_000690"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP9775570.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            32753..33697
                     /gene="ydgH"
                     /locus_tag="ACHOS5_000691"
     CDS             32753..33697
                     /gene="ydgH"
                     /locus_tag="ACHOS5_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP9775571.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVITG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            33883..35265
                     /gene="ydgI"
                     /locus_tag="ACHOS5_000692"
     CDS             33883..35265
                     /gene="ydgI"
                     /locus_tag="ACHOS5_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9775572.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            35302..36024
                     /gene="folM"
                     /locus_tag="ACHOS5_000693"
     CDS             35302..36024
                     /gene="folM"
                     /locus_tag="ACHOS5_000693"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP9775573.1"
                     /translation="MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(36021..36356)
                     /gene="ydgC"
                     /locus_tag="ACHOS5_000694"
     CDS             complement(36021..36356)
                     /gene="ydgC"
                     /locus_tag="ACHOS5_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP9775574.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            36485..37204
                     /gene="rstA"
                     /locus_tag="ACHOS5_000695"
     CDS             36485..37204
                     /gene="rstA"
                     /locus_tag="ACHOS5_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP9775575.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            37208..38509
                     /gene="rstB"
                     /locus_tag="ACHOS5_000696"
     CDS             37208..38509
                     /gene="rstB"
                     /locus_tag="ACHOS5_000696"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP9775576.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            38585..39514
                     /gene="tus"
                     /locus_tag="ACHOS5_000697"
     CDS             38585..39514
                     /gene="tus"
                     /locus_tag="ACHOS5_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP9775577.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(39511..40914)
                     /gene="fumC"
                     /locus_tag="ACHOS5_000698"
     CDS             complement(39511..40914)
                     /gene="fumC"
                     /locus_tag="ACHOS5_000698"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP9775578.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(41057..42703)
                     /gene="fumB"
                     /locus_tag="ACHOS5_000699"
     CDS             complement(41057..42703)
                     /gene="fumB"
                     /locus_tag="ACHOS5_000699"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9775579.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            42902..44077
                     /gene="manA"
                     /locus_tag="ACHOS5_000700"
     CDS             42902..44077
                     /gene="manA"
                     /locus_tag="ACHOS5_000700"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP9775580.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            44178..45686
                     /gene="ydgA"
                     /locus_tag="ACHOS5_000701"
     CDS             44178..45686
                     /gene="ydgA"
                     /locus_tag="ACHOS5_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9775581.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(45731..46996)
                     /gene="uidC"
                     /locus_tag="ACHOS5_000702"
     CDS             complement(45731..46996)
                     /gene="uidC"
                     /locus_tag="ACHOS5_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP9775582.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(47035..48408)
                     /gene="uidB"
                     /locus_tag="ACHOS5_000703"
     CDS             complement(47035..48408)
                     /gene="uidB"
                     /locus_tag="ACHOS5_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP9775583.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            complement(48405..50216)
                     /gene="uidA"
                     /locus_tag="ACHOS5_000704"
     CDS             complement(48405..50216)
                     /gene="uidA"
                     /locus_tag="ACHOS5_000704"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9775584.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVGANGDVSVELRD
                     ADQQVVATGQGTCGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(50606..51196)
                     /gene="uidR"
                     /locus_tag="ACHOS5_000705"
     CDS             complement(50606..51196)
                     /gene="uidR"
                     /locus_tag="ACHOS5_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP9775585.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEVFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(51425..52192)
                     /gene="hdhA"
                     /locus_tag="ACHOS5_000706"
     CDS             complement(51425..52192)
                     /gene="hdhA"
                     /locus_tag="ACHOS5_000706"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP9775586.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVNEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(52304..53332)
                     /gene="malI"
                     /locus_tag="ACHOS5_000707"
     CDS             complement(52304..53332)
                     /gene="malI"
                     /locus_tag="ACHOS5_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP9775587.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            53507..55099
                     /gene="malX"
                     /locus_tag="ACHOS5_000708"
     CDS             53507..55099
                     /gene="malX"
                     /locus_tag="ACHOS5_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP9775588.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            55109..56281
                     /gene="malY"
                     /locus_tag="ACHOS5_000709"
     CDS             55109..56281
                     /gene="malY"
                     /locus_tag="ACHOS5_000709"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP9775589.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFIRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            56385..57386
                     /gene="add"
                     /locus_tag="ACHOS5_000710"
     CDS             56385..57386
                     /gene="add"
                     /locus_tag="ACHOS5_000710"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9775590.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(57422..58462)
                     /gene="ydgJ"
                     /locus_tag="ACHOS5_000711"
     CDS             complement(57422..58462)
                     /gene="ydgJ"
                     /locus_tag="ACHOS5_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9775591.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEIAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            58705..58830
                     /gene="blr"
                     /locus_tag="ACHOS5_000712"
     CDS             58705..58830
                     /gene="blr"
                     /locus_tag="ACHOS5_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP9775592.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            59103..59318
                     /gene="ydgT"
                     /locus_tag="ACHOS5_000713"
     CDS             59103..59318
                     /gene="ydgT"
                     /locus_tag="ACHOS5_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP9775593.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWRYVQ"
     gene            59404..59844
                     /gene="ydgK"
                     /locus_tag="ACHOS5_000714"
     CDS             59404..59844
                     /gene="ydgK"
                     /locus_tag="ACHOS5_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP9775594.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            59921..60502
                     /gene="rsxA"
                     /locus_tag="ACHOS5_000715"
     CDS             59921..60502
                     /gene="rsxA"
                     /locus_tag="ACHOS5_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP9775595.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            60502..61080
                     /gene="rsxB"
                     /locus_tag="ACHOS5_000716"
     CDS             60502..61080
                     /gene="rsxB"
                     /locus_tag="ACHOS5_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP9775596.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            61073..>62949
                     /gene="rsxC"
                     /locus_tag="ACHOS5_000717"
     CDS             61073..>62949
                     /gene="rsxC"
                     /locus_tag="ACHOS5_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9775597.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKIQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKVTTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AA"
BASE COUNT        15615 a        16232 c        15268 g        15834 t
ORIGIN      
        1 taattttgct ggcgactgcg aaacggcatt cgcattagcg gtgaagtttg ccctcgccca
       61 gttgctgcaa ttactgctat aacgaagctg gtctggcgat aaatccaggc cagccattgg
      121 tggtgtttat acgttcaagc cacgatgttg cagcatcggc ataatctgag gtgccttacc
      181 gcgccattgt cgatacaggc gttccagatc ttcgctgtta cctctggaaa gaatcgcctc
      241 gcgaaaacgc cgcccatttt cacgcgttaa tccgccctgc tcaacaaacc actggtaacc
      301 atcatcggcc aacatttgcg tccacagata agcgtaataa cctgcggcat atccgccacc
      361 aaaaatatgg gcgaaataac tgctgcgata gcgtggcggt atagcaggaa gatccatatt
      421 ttccgccacc agcgcccgca attcgaaatc atcgacgtcc tgcattgctt cgttttcttc
      481 cagacaatgc cagcgcatat cgagaagtgc ggcgctaagc agttcgctca tctcataccc
      541 tttattgaac agactggcat tacgcatttt ctgttgaagt tcgtcaggca ttgcggcccc
      601 gctctgataa tgccgggcgt agcgagcaaa tacctgcgga tgtgttgccc agtgttcgtt
      661 gatttgcgac ggaaattcga caaaatcacg cggcgtgttg gtgccggaaa gcgtggcata
      721 acgctggcgg gcaaaaaggc cgtgcagcgt atgaccaaat tcatggaata aggttattac
      781 atcatcccag agtaacaacg caggctcacc ggcagcgggt ttctgataat tgcagacgtt
      841 ataaattacc gggtgcgttt cattcagcgt tgattgctca acaaaattgc ccatccatgc
      901 accgccgctt tttgaatcac gggcgaagaa atcaccgtaa aataacgcca gccccacgcc
      961 attatgatca aaaatttccc acactcgaac gtcaggatgg tagacaggaa tatcaaaacg
     1021 ttcgacaaac ttaataccga agagctgatt cgcggtccag aatacacctt cattcaacac
     1081 cgtgtttaat tcaaaatatg gcttgagctg cgattcatcg agatcgaatt tctcacgtct
     1141 tacctgctcg gcataaaatg cccagtccca cggctgcgcg ctaaacccgc cctgctgctt
     1201 atcgataacc gcctgtatcg aggctaattc atcgctcgca cgttgacgcg ccgctggaac
     1261 aatttcccgc ataaagttga gcgctgcttc tggcgttttt gccatctgat cggcgatttt
     1321 ccatgcggca taatgaggaa aaccaagcag tttcgcctgc tgcgcgcgga tctccaccag
     1381 acgttgaatg atagtgcggg tatcattggc atcatttttt tccgctcgcg tccagcccgc
     1441 agtaaacagt ttttcacgcg tcgcgcgatc gcgcagttca gcaagcgctg gttgctgggt
     1501 ggtattcagc agcggaatca gccagttgtt atccagacct ttctcgcgag ccgcctctgc
     1561 cgcctgcgca atctcttgtt cactcattcc tgccagctgc gcgacatcgt tcacaaccag
     1621 accgccggat ttatttgctg ccagtaaccg ctggttaaac tggctggtca gggtcgcagc
     1681 ttctgtattc agtactttta attttgcttt atcagcttgc tcaagtttgg ctccggcaag
     1741 gacaaaacgt tgatgaatca cctccaccag gcggatggat tcactatcaa gccccaggga
     1801 ttcacggcgt tgccagacag catctacccg cgcgaataat tccccgttca gatagatatc
     1861 attcgcaagt tccgccagtt cagcggaaaa ctgttcatca agacgctgta attcatcatt
     1921 ggtatgcgcc gcagtcatcg caaaaaagac gctggtaacg cgggtaagta attctccgct
     1981 ttgttccagt gccagaatag tatttttaaa atcaggtgtt tgcgggttaa gcgcgatggc
     2041 agcaatttct gcccgctttt gctgtattcc ctcatcgaat gccgggcgat agtgatggtt
     2101 ggcaatttga tcaaaatggg gagccagata cggcagtgtg ctttgcacaa ggaaaggatt
     2161 cattgttgtc attttcttct cctgaacgcg aggtgttcca tagcgtaggc ttactgataa
     2221 cggagtgcaa tcttgcaaac cagtattacc cgctcttaag catcacgtgc tatgttattg
     2281 acacacaaaa gcgttgagga acagtgagat gatcgtttta gtaactggag caacggcagg
     2341 ttttggtgaa tgcattactc gtcgttttat tcaacaaggg cataaagtta tcgccactgg
     2401 ccgtcgccag gagcggttac aggagttaaa agacgaactg ggagataatc tgtatatcgc
     2461 ccaactggac gttcgcaacc gcgccgctat tgaagagatg ctggcatcgc tccctgccga
     2521 gtggagcaat attgatatcc tggtaaacaa tgccggcctg gcgttgggca tggagcctgc
     2581 gcataaagcc agcattgaag actgggaaac gatgattgat accaacaaca aaggcctggt
     2641 atatatgacg cgcgccgtct taccgggtat ggttgaacgt aatcatggtc atattattaa
     2701 cattggctca acggcaggta gctggccgta tgccggtggt aacgtttacg gtgcgacgaa
     2761 agcgtttgtt cgtcaattta gcctgaatct gcgtacggac ctgcatggta cggcggtgcg
     2821 cgtcaccgac atcgaaccgg gtctggtggg tggcaccgag ttttccaatg tccgctttaa
     2881 aggcgatgac ggtaaagcgg aaaaaaccta tcaaaatacc gttgcattga cgccagaaga
     2941 tgtcagcgaa gccgtctggt gggtgtcaac gctgcctgct cacgtcaata tcaataccct
     3001 ggaaatgatg ccggttaccc aaagctatgc cggactgaat gtccaccgtc agtaattttt
     3061 atacccggcg taactgccgg gttattgctt gtcacaaaaa agtggtagac tcatgcagtt
     3121 aactcactca caagcaagaa cgaatgaccg tcgaaacgca acttaatccc acacagcctg
     3181 tcaatcagca gatttatcgt attcttcgcc gcgacattgt ccattgcctg attgcaccag
     3241 gcacaccgtt gtcggaaaaa gaagtttctg ttcgtttcaa tgtgtcacgc cagccggttc
     3301 gtgaagcctt tattaaactg gcggaaaacg gtctgattca aattcgtccg cagcgtggca
     3361 gctacgtcaa caaaatttcc atggcccagg tgcgcaacgg cagttttatc cgtcaggcca
     3421 ttgagtgcgc ggtggcgcgt cgggcggcga gcatgattac cgaaagccag tgctaccaac
     3481 tggaacaaaa tcttcaccag caacgcattg ccattgagcg caagcaactg gatgattttt
     3541 ttgaacttga tgacaacttc catcaactcc tgacgcagat tgccgactgt caactggcgt
     3601 gggataccat tgagaacctg aaagcgaccg ttgatcgcgt gcgctatatg agtttcgacc
     3661 acgtttctcc accagaaatg ctgttacgcc agcatcttga tattttctct gccctgcaaa
     3721 aacgtgatgg cgatgcggta gaacgtgcaa tgacgcaaca tttgcaggaa atcagcgaat
     3781 ctgtgcgcca gatccgccag gaaaacagcg actggtttag cgaagagtaa ttcatttcct
     3841 ctcatcccat ccggggtgag agtcttttcc cccgacttat ggctcatgca tgcatcaaaa
     3901 aagatgtgag cttgatcaaa aacaaaaaat ttttcactcg acaggagtat ttatattgcg
     3961 cccgttacgt gggcttcgac tgtaaatcag aaaggagaaa acacctatga cgacctacga
     4021 tcgtaaccgt aacgcaatca ccactggcag ccgtgttatg gttagcggca ccggtcacac
     4081 tggcaagatc ctgtcgattg atactgaagg tctgaccgct gagcaaatcc gccgcggaaa
     4141 aaccgtagtt gttgaaggtt gtgaagagaa actggcacca ctggacctga ttcgtctcgg
     4201 catgaactaa gcgtgtgaat gccgccgatg gcggcattgc tttttttact tcacggaata
     4261 tttcgccacg gtcgctttcg cgccatgcgc taataaagac aagtacgctt ccgttacttt
     4321 tgtcgtaaac aaactattgc ctggtaaatc atcaccaaag atcgccttaa ttgccagcag
     4381 tgactggacg cgcgctgtcc cttcagcact actttgtact gccttctgaa taacaggtaa
     4441 cagtggatca ctgatttcta tcggatttcc ctgttcatca acaccaccga cataacgcat
     4501 ccaacccgcg acgcccagcg ccagcagatc gaacttgctg tcatgcgcca gatgccagcg
     4561 aacagaatcc aacatccgct gtggcaattt ctggctgcca tccatcgcaa tctgccaggt
     4621 tcgatgacgt aacgccgggt tgctatagcg ttcaattaat cggttagcgt aatcctgcaa
     4681 atcaacgccc tgcactttca gcgtcggcgc ttgttcctgc aacattaagg tatacgccgc
     4741 atgacgataa tgttcatcct ccatgcagtc attaatgtgc tgatatccgg caagatagcc
     4801 cagatacgcc aggaatgaat gactgccgtt aagcatacgc agtttcattt cttcataagg
     4861 cagcacatcg ctaaccagtt cagctcccgc tttttcccat tccggacgtc cggcaacaaa
     4921 gttatcttct attacccact ggcggaaagg ttcacaggca acgcctgccg gatcgcgcac
     4981 accagtaagt tgttcgattt tcgccagcgt atcctctgtc actgcgggca caatacggtc
     5041 caccattgtt gatgggaaag tcacgttatc ttcgatccat tgtgccagtt ttacatcaat
     5101 ggcttgcaca taggaagtga caacgtcacg cataacatga ccgttttctg gcatgttgtc
     5161 acatgacatg acggtaaatg cgggaagtcc tgccgcttta cggcgagcca gcgcctcaac
     5221 aatcatccct gttgcagttt tcggctggtg gggattttgc acgtcggcaa cgaccatcgg
     5281 gtgatcaagc attaactgtc cggtcgccgg agagtggaaa tacccttttt cggtgattgt
     5341 cagagagaca atcgcgattt gcggttcaca catcgcagcc aacactgttt ctaagccatc
     5401 tatttgtacg tgtaaggctt ttttaacgac gccaacgacg cgacccgtcc acgcatcagc
     5461 cgacatttcc gcaacggtat aaagattatc ttgctgttgt aaatcggcaa tttgctgttc
     5521 gccgccgatt aagttgacct cataatatcc ccagtcactg aaatgttccg tagcaagaat
     5581 atcggcatac acgccctgat gcgcacggtg aaatgcacca aagcctaaat gaacaattct
     5641 tggggccagg ttattacgat cataaacagg gagcgtcgct tttgctgata acaaatgatt
     5701 tcccataaca attccttaaa attaaatata gcaagctaca tgttttgtta tatgaataaa
     5761 aatcccctct ccggtaagag aagggattga ggatttacag acttctggac ggttgcgcag
     5821 ctcttacaac atgtggttga tcttctgcgg cgtcttccag cgcacttaaa tcacggtctt
     5881 tcacttctgg cattttcagc gcagagatta aaccaatcac agaatatgcc atgatcataa
     5941 tggcgatcgg ataccaggat tccgtcatgg tgcagaaaat ccccgccagg ataggaccaa
     6001 aaccggaagc gataagacca ccaatttctt tagaaatagc cattcgggta aagcggtttt
     6061 tacagccgaa catttctgcc atggtaatgt tttccagagc aaataatcca agcaccgcac
     6121 agttatgaat cacaatcagt gcaaccataa tggtgctcgg ggcatagctt ttatctacga
     6181 tgatagaaag cattggccat gccagcacaa ttgcagaggt attcataata atatacggga
     6241 tccggcgacc aattttatcg gataaccaac caaggaacgg aatggtcata aagccgagaa
     6301 tcgaactgat catcaatgca tctgttggaa ttgctttgtt aaacaataac gtctgcacta
     6361 aatagcctgc aaggaaagtc tgaattaaac ctgagttacc agcctgacca aaacgcagcc
     6421 ctgttgccag ccagaaggat ttgctctgga acatgctacc agcaggtgca ggttttgctg
     6481 tcggttggtt actgtcgtta accttctcaa agaccgggct ttctttcaga ttcatacgca
     6541 accagatagc aaagaccatc acgacaacgc tcgccaggaa cggtatacgc catccccacg
     6601 ccagcagttc ctctttactg agaatgaaga acataaaggc ccagattgcc gttgcgctca
     6661 aggttccgca gttagttccc atagccacaa atgaggagat aattccgcgc ttaccttttg
     6721 gtgcatattc cgccagcatc gtgccggcac cggaaatttc cgcgcctgcc cccagcccct
     6781 gaataatacg cagcgtcacc agcaaaattg gcgcaaacac accaatttgt gcataggtcg
     6841 gcagcacacc aattaaggtg gtacagatcc ccatcatagt gatggtaata aagagcactt
     6901 ttttacgccc gattctgtcg cccattttgc cgaaaataaa tgctccgaca atacgcgcca
     6961 cataacctgc accgtaggtt cccattgcca gaattaacgc cattgccgtt gatgattcag
     7021 gaaaaaatat ttcatgaaac actaacgctg cgccgagcga atataactgg aaatccataa
     7081 attcaagcgc agtgcccagc catcccgata ctgctgcttt caccaaatcc ttagtacttc
     7141 tttcgtgttt ttctattgtc ataatggtta tctctaaaaa agaggtaaga tgcgtactac
     7201 ttactcgccg ttattggtat tattcagaaa aagtgagtaa gactttgcag caatgttttt
     7261 gatcctgttc aaataaacta atggcatccg caacatgctg gaaatcaaat gtatgggtaa
     7321 ttagtttttc tggtttaatt aaccctttac ttaaccagtc gataacgacc gggaatttat
     7381 ttgcatttaa gcgtgaagag aaaatagaga gttcttttcc ggtaattcct tgctgaatca
     7441 cttcagacgg ttcactggag aagcccatca atacaatacg tgccgcagga gaagccagcg
     7501 ttacggcctc tttcaagata gaaggatgac aagcggcatc gataattaaa gtcggcttga
     7561 tgcctttttc agcgaaactc tcgccaagcg gtgtctggct gttattaatc gcccagtctg
     7621 ccccgctctc tttcgctttt tccagtcgtt catcaatgcg atcggcaaca atcacatttt
     7681 taacgttata gacgcctttt aatacctgaa caatcgtcag gccgattgga cctgcaccgt
     7741 aaaccagaac ggtatcattt tcagtcggtt gaccatgacc ggtaacgtta gccgcaatgg
     7801 taaaaggttc gatcatcacc gcatattgat cggccactgc ttcgggaatt ttccacgcat
     7861 tttttgccgg caccacggca tattcactga aaccaccgtc agcgtgcaca cctaatacag
     7921 ccagtgtcgt acaaacgttc ggcttaccta tagagcacgg atagcaatgc ccacagctga
     7981 ccaccggatc gacagcaacg cgttcaccga ctctggcgct ttccacgcct tcacccactg
     8041 catcaatgac gccaaagaat tcatgaccaa tgacgcgcgg atatttcgca aaaggattat
     8101 gcccacgata aatatggcta tctgaaccac aaattccggc aagtttcact tttactcgta
     8161 cttcacccgc tgacggggtg ggtatttcgc gttcgataat tgccagttga ttcggttttt
     8221 caattaaaat gcttttcatt atcttactcc ttaccagttc cacagcgtgc catcttccag
     8281 acgtgcgact ggcagataag caggttcata gggatatttc gccgccagct tttcatcgaa
     8341 ttcgatgcca aggcccggtt tttctcccgg atgcatatag ccgttatcga aagtccagtt
     8401 gtgcgggaag acttcgagca tttgttcgga ataacccatg tattcctgga caccgaaatt
     8461 ggggacccac agatcaaagt gcagcgccgc agccatgcag actggtgaca aatcggaagg
     8521 accgtgtgag ccagtacgta cctgatacag cgaagcaaaa tcggcaatcc ggcgcatacc
     8581 ggtaattccg cctgcatggg tcagcgtggt acggatataa tcgatgagtt gctcttcaat
     8641 cagttgtttg cagtcccaga tgctgttgaa gacttcaccc accgcgatgg gtgtgacggt
     8701 atgttggcga atgagacgga agcattcctg gttttccgca ggcgtcgggt cttccatcca
     8761 gaacatgcga taatcttcaa tgctcttacc aaagcgcgcc gcttcaatag gcgttaagcg
     8821 atggtgcatg tcatgcagca gatgttcatc aaaaccaaac ttgttacgta ccgcgtcaaa
     8881 caatttcggc atgaaatcga ggtatttctc cgtcgaccac agctgctctt ccggccactg
     8941 tcctttggtt gcgggttcat aagccagacc tttacctttc gacatgccgt aggtggtttt
     9001 cataccaggg attccgcact gcacgcggat ggctttgaat cccagctcct gatgacgggc
     9061 ataatcatcc agagcttcat caatactgtg accggtggta tggcaataaa ccatcacccc
     9121 ttcacgagac gcgccgccga gtaactgata aagcggcatg ttggcagctt tggctttaat
     9181 atcccacagc gccatatcaa ccgctgaaat ggccgacatc gtaaccggac cgcgacgcca
     9241 gtaagcacct ttatagaaaa actgccagat atcttcgata cggtgtgcat cgcgaccaat
     9301 aagctgcgga caaaggtgat cctgcaaata agaggccacg gaaagctcac gtccatttag
     9361 ggtggcatcc ccaaggcccg taataccgtc ctcagtggtg atttttaatg tgacgaaatt
     9421 acgccccgga caggtaacaa aaacttcagc ctttacgatc ttcatgttcg attccttgca
     9481 tcgcttgtcg tgatgcatga aatctacgca actgagctac taccatacaa gtatgaagat
     9541 caaaaaaagc cggagtgatc acaaaaaaag gcgtatattt gcgccgtgaa tggttgacaa
     9601 aagatgaaat agaatacctt ttgtcagctg acacttcctc gtatcttatt gataaaatgg
     9661 atttatgttc ctacgtgcgc ccccagcccg caacaatgat caacatgccg caaagcgcaa
     9721 ttaacgcccc cgtccagtca taaagagtca gtttcacgcc atccacaacg cgcagccaca
     9781 tcaaagccgt gcagacataa acgccaccat aagccgcgta aacacgccca ctcgccgcag
     9841 gatgcaacgt taacaaccag acaaacagcg ccagtgaaat ccccgccgga agcaacagcc
     9901 agatactggc gtttcgcttc aaccacagcc agggcaaaaa gcatccaata atttcacaca
     9961 gcgcagtagc aaaaaatagt aacgttgttt taatcatctt tgtcacttat tgacatcacg
    10021 tatagttata gggcgacata atatcatcaa tataaacacc ctccttgaac gttttgcgtt
    10081 cgcagtggat ggtgtagaat cagctttaat cattcataca gggaatgaat tatgaaaatc
    10141 actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact ggcattgagc
    10201 acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag tgcacaaagc
    10261 cgccagcgcg ccgctatgga aaaagagcaa tggaatgaca cgcgtaatct gcgccagaaa
    10321 gtgaataaac gcactgaaaa agagtgggat aaagccgacg ctgcttttga taaccgcgat
    10381 aaatgtgagc aaagcgccaa catcaatgcc tactgggagc caaatacttt gcgctgcctg
    10441 gaccgtcgaa ctggccgcgt tattatcccc taacctgtta ttgatttaag gaatgtaagg
    10501 acacgttatg ccaagcgccc acagtgttaa gctacgcccg ctggagcgtg aagatttacg
    10561 ctatgtacat caactcgaca ataacgccag tgtgatgcgt tactggtttg aggaacccta
    10621 cgaagccttt gttgaactct ctgatctgta tgataagcat attcacgatc agagcgaacg
    10681 gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg tggaaattaa
    10741 ccatgttcat cgccgcgcag aatttcagat aattatctcc ccggagtatc aggggaaagg
    10801 tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc tcaatctcta
    10861 taagctgtat ctgatcgttg ataaagagaa tgaaaaagcg attcacattt accgcaagct
    10921 tggctttacg gttgaaggtg aattgatgca tgagttcttt attaatggtc aatatcgtaa
    10981 tgccattcgc atgtgtatat tccagcatca gtatctggct gagcacaaaa caccgggtca
    11041 gactctcctg aagccgaccg cacaatagta ttaataataa tctatcgtat ttttgatggt
    11101 gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgattagct gacagtccac
    11161 cggattggcg tgattgtcat attcacagct ctgttttaca tttccaaccc gttgattatt
    11221 cagtaaagta accgctgtgt aatctaattt tttgatcggg tccgttgatg gcgtggcgct
    11281 gacagataat gttttatcgt tacttttcgt ggttttaccc agcggatacc cctgatcatc
    11341 atagcgatat tccatctgca tctgtttgct gctggcttta atcacgaaac cattatcatc
    11401 ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc cctgcaaacg
    11461 aactcttttc tccagcgtct cggcatcacg gtaataattg gcgtccagta ccagggcaac
    11521 cacggtatta ttttccagat ccagtaattc gagagaatca aaacagcctt cttccgacaa
    11581 agtccccgaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct gagtgaaatc
    11641 ttttacggga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg ccatctccgg
    11701 ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg agtaatatcg cgagtaagca
    11761 tggcagtaat ttgtatttca caacagtcac ctgagagtag agatgattct caatcatagt
    11821 agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct tcgcgtaagg
    11881 agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc gcctcaccca
    11941 cggcactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca cagcgcatta
    12001 tcaataatgg cgtacccgaa aacagcttca cgttaagtat tgtacccaat gaccaggttg
    12061 atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa attctctaca
    12121 cccgtaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat aacgcgcctg
    12181 ctgaaccgca gtaacacatt atcgactgaa cgccggatac gacacatccg gcgatttgaa
    12241 catacaacat aatcccacct tattactcat acccttctat tgatatggat taataattat
    12301 taaccccaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa tatataaaca
    12361 tttatataac gataaaaaac agggagtgag ttatgtccaa aagtaaacaa atggtgggca
    12421 tcagccgcag aacactcgtt aaatctaccg cgataggttc tctggcgctg gctgcaggcg
    12481 gtttttcttt gccgtttacc ctgcgcagtg cagcagcagc ggtacaacag gccagcgaaa
    12541 aagtggtctg gggtgcctgt tccgtcaact gtggtagccg ctgtgcactt cgtctgcatg
    12601 ttaaagataa tgaagtgacc tgggtggaaa ctgacaacac cggcagcgac gagtatggca
    12661 accatcaggt acgcgcctgt ttgcgcggtc gctccatccg tcggcgtatt aatcatcccg
    12721 atcgcctgaa ttacccaatg aaacgcgtgg gcaaacgcgg cgaaggcaaa ttcgaacgga
    12781 ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc gtcaaacaat
    12841 atggcaatga ggctgtatat attcagtact cttcggggat tgttggcggc aatatgaccc
    12901 gctcttctcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc ggttcactaa
    12961 atcagtatgg ctcctacagc actgcgcaaa tttcctgtgc catgccctac acctacggca
    13021 gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg atgtttggta
    13081 acaacccggc tgaaacccgc atgagcggcg gtggcatcac ttatcttctt gaaaaagcgc
    13141 gcgagaaatc gaacgcaaaa atgattgtta tcgatccgcg atataccgat acggctgctg
    13201 gtcgtgaaga tgaatggctt cctattcgcc cgggcaccga tgccgcgctg gttgcgggta
    13261 ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat aaatactgcg
    13321 tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac tataaagcct
    13381 atattcttgg tgaaggtgat gataaaacag cgaaaacgcc gcagtgggct tcgcaaatta
    13441 caggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca gcaaaacccg
    13501 cttatatctg ccagggctgg gggccgcaac gccaggcaaa cggcgagctg acggcacgcg
    13561 ctattgctat gttgcctatt ttgacgggca atgtcggcat cagcggcgga aatagtggtg
    13621 cgcgtgaatc gacctacacc attaccatcg aacgcttgcc ggtgctggat aatccggtca
    13681 aaacgtcaat ctcctgcttc agctggacag atgctatcga tcatggcccg caaatgacgg
    13741 caatccgcga cggcgtccgg ggcaaagata aactagatgt gcccattaag ttcatctgga
    13801 actacgcggg aaataccctg gttaatcagc attctgacat caacaaaacg catgaaattc
    13861 tgcaggacga atcgaaatgc gaaatgattg tggtcatcga aaactttatg acctcatcgg
    13921 caaaatatgc cgacattctg ctgccagacc tgatgaccgt tgagcaggaa gatattattc
    13981 ctaacgacta cgccggtaac atgggatatc tcattttcct ccagcctgtc accagcgaaa
    14041 aattcgaacg caaaccaatt tactggatcc tgagtgaagt cgcgaaacgt cttggacctg
    14101 acgtctatca aaagttcact gaaggtcgca cgcaggaaca atggttacaa catctgtacg
    14161 ccaaaatgct tgccaaagat ccggcattac cgtcttacga cgaattaaaa aaaatgggta
    14221 tctataagcg taaagatccc aatggtcatt ttgtcgccta caaagcattt cgtgacgacc
    14281 ccgaggcaaa tccacttaaa acgccttccg gtaagattga aatttattcc agcaagctgg
    14341 cggaaattgc ccgtacctgg gaactggaaa aagatgaagt gataagccca ctgccggttt
    14401 atgcctcaac ctttgaaggc tgggactccc ctgaacgtag tgccttccca ctgcaactgt
    14461 ttggtttcca ttacaaatcc cgtacgcact cgacctacgg caacattgat gtcctgaagg
    14521 cagcctgccg tcaggaagtg tggatcaacc ctatagatgc acagaaacgt gggattgcca
    14581 acggcgatat ggtgcgggtg tttaaccatc gtggcgaagt tcggctacca gccaaagtga
    14641 caccacgcat tctccctgga gttagtgcta tgggccaggg agcctggcac gaggccaata
    14701 tgtctggcga taaaatcgac cacggcggct gtgtgaatac gctaaccact ctgcgccctt
    14761 caccactggc gaagggaaac ccgcagcaca ctaatctggt cgagatcgaa aaaatataac
    14821 ccactacgac cataaatttt gacatgacat tttgtttgaa aagcaataag tgagtaatga
    14881 tgaaaatcca taacacagag gcgctcatgt cggctgaaat cagtcgtaga agtctgatga
    14941 aaacctccgc acttggcagt ctcgcgctgg caagcagtgc tttcactttg ccgttttccc
    15001 aaatggtccg agcggcacag gcaccggtag aagagaaagc ggtctggagt tcctgcaccg
    15061 ttaactgcgg gagccgctgt ctgttacgtt tgcatgtgaa agatgacacc gtgtactggg
    15121 tggagtctga tacgacaggt gacgacgttt acggtaatca tcaggttcgg gcatgtttac
    15181 gcgggcgctc tatccgccga cggatgaatc atcctgacag gttgaaatat cccatgaagc
    15241 gcgtcggcaa gcgcggtgaa ggtaaatttg aacggataag ttgggacgaa gccctggata
    15301 ccatcagtga taatcttcgg cggatcctaa aagattacgg caatgaggct gtacatgttc
    15361 tctatggaac aggcgtagat ggcggaaaca tcaccaactc aaacgtcccg taccgtctga
    15421 tgaactcttg cggtggtttt ctcagtcgct atggcagcta tagtaccgca cagatcagtg
    15481 ccgcaatgag ttatatgttc ggtgccaatg atggcaacag cccggatgat atcgccaata
    15541 cgaaactggt cgttatgttc ggaaataacc cggcagaaac acggatgagc ggcggtggtg
    15601 tcacttacta cgtcgagcaa gcccgcgaac gttcaaacgc acgcatgatc gtcatcgatc
    15661 cacgttataa cgacacagct gccgggcgtg aagatgaatg gctgcccatt cgccctggca
    15721 ccgatggcgc actggcctgt gcgattgcct gggtactgat tactgaaaac atggtcgatc
    15781 agccatttct cgacaaatat tgtgttggtt acgatgaaaa aacgctgcca gctaacgctc
    15841 cacgtaacgc gcattataaa gcctatattc tgggcgaagg gactgacagc atagctaaga
    15901 cgccggaatg ggcagcaaaa atcaccagca tcccggcaga aaaaattatc cagttggcac
    15961 gagagatcgg ttcagcaaaa ccggcttata tttgtcaggg ttgggggcca caacgacatt
    16021 ccaacggcga gcaaacgtcc cgcgctattg ccatgctttc cgttctcacc ggtaacgtcg
    16081 gcataaacgg cggcaactca ggtgtacgcg aaggtagctg ggatctgggg gtagaatggt
    16141 tcccgatgct cgagaatcca gttaaaacgc agatttccgt ctttacatgg acagatgcca
    16201 tcgaccatgg tacggaaatg accgcgaccc gtgatggtgt tcgaggaaaa gaaaaactgg
    16261 atgtccccat caagttttta tggtgctacg ccagtaacac attgatcaat caacatggcg
    16321 acatcaatca cacccatgag gtgcttcagg atgacagcaa gtgcgagatg attgttggca
    16381 ttgaccactt catgactgcc tcggctaagt attgcgatat cctgttaccc gacctgatgc
    16441 caacagagca agaagacctt atctctcatg agtctgccgg gaatatgggc tatgtgatcc
    16501 tcgcccaacc cgcaacctca gcaaaatttg aacgcaaacc catctactgg atgctgagcg
    16561 aagtcgccaa acgcttagga ccagacgttt atcaaacctt tactgaaggt cgcagtcagc
    16621 atgaatggat caaatatctc catgcgaaaa cgaaggaacg taaccctgag atgcccgact
    16681 acgaggagat gaaaacgacc gggatcttta agaaaaaatg cccggaagaa cactacgtcg
    16741 ctttccgcgc attccgtgta gatccacagg caaacccatt gaaaacacct tcgggaaaaa
    16801 tcgaaattta ttctgaacga ctggcgaaga ttgcggatac ctgggaattg aaaaaagatg
    16861 aaattattca tcctcttcca gcttatacgc caggttttga tggctgggac gatcccctgc
    16921 ggaaaactta tccactgcag ttaacgggct tccactacaa agcgcgtacc cactccagtt
    16981 acggcaatat tgatgtatta cagcaggcct gcccacaaga ggtgtggatc aaccccattg
    17041 atgctcaggc acgcggtatc cgtcatggcg ataccgtgcg ggtatttaac aataatggag
    17101 aaatgctgat tgccgcaaaa gtgactccgc gtattctgcc tggcgttacc gccatcgggc
    17161 aaggtgcttg gcttaaagcg gatatgtttg gtgaccgggt cgatcacggc ggcagtatca
    17221 atattctgac ctctcaccgc ccttcaccgc tggcaaaggg aaacccgtcg cacagcaatc
    17281 ttgtccagat cgaaaaggtt taaggagtag cccatgacca cacaatatgg attttttatc
    17341 gattccagcc gctgtactgg ctgtaaaacc tgcgaactgg cgtgcaaaga ttttaaagat
    17401 cttggcccgg aagtcagttt ccgccgtatt tatgaatatg ctggcggcga ctggcaggaa
    17461 gataatggcg tctggcacca gaacgtgttc gcttattatc tctccatctc ctgtaaccat
    17521 tgcgatgacc ccgcatgtac aaaagtctgc cccagcggtg caatgcataa gcgtgaagat
    17581 ggtttcgtgg tggttgatga agatgtttgt atcggctgcc gctactgcca catggcttgt
    17641 ccgtacggcg cgccacagta caatgctgaa aaagggcaca tgacgaagtg cgatggttgt
    17701 tattcgcgcg tcgccgaggg gaaacaaccc atatgtgtcg aatcctgccc actgcgtgca
    17761 cttgagttcg gtccaattga agaattgcgc cagaagcacg gcactctggc tgccgtcgcg
    17821 ccactgccgc gcgcgcattt cacaaaaccc aatatcgtta tcaaacctaa cgccaacagc
    17881 cgcccgaccg gcgataccac tggttatctg gctaatccgg aggaagtgta aaatgggaaa
    17941 tggatggcat gaatggccac tggttatctt taccgtattg ggtcaatgcg tagtaggcgc
    18001 gctgattgtt agcggaattg gctggttcgc cgccaaaaat gacgcagacc gtcagcacat
    18061 tattcgtggc atgtttttcc tctggctgtt gatgggcatc gggtttattg cctctgtcat
    18121 gcacctggga tcgccactgc gggcgtttaa ctcgctgaat agaatcggag cttctggtct
    18181 gagtaacgag attgctgccg gttccatatt ttttgccgtt ggcggattat ggtggctggt
    18241 ggcggtcatc ggtaaaatgc cgcaagcgtt ggggaaactc tggttacttg tcagcatggc
    18301 gctcggcgtc attttcgtct ggatgatgac ctgcgtgtat caaatcgaca ctgtgccaac
    18361 ctggcataac ggttacacta cgctggcttt cttcctgacg gtattgttga gcggtccaat
    18421 acttgctgcc acaattctac gtgcagcccg cgttacattt aataccacac catttgccat
    18481 cattagcatt ctggcgctga tcgcttgtgc cgggttgatt gtgctgcaag gtttgtctct
    18541 ggcgtctatt cactcatccg tacaacaagc cagcgcactg gtaccagatt acgcctcctt
    18601 acaggtctgg cgtgtggtat tgttatgcgc aggtcttgga tgctggcttt gtccgctcat
    18661 tcgtcgccgt gaacctcacg ttgccggact tgttctgggg ttaatcctca ttcttggcgg
    18721 tgagatgatc gggcgtgtgc tcttttattg cctgcacatg acggtaggaa tggctattgc
    18781 aggttaacaa attggtgcgg ggcaacccgc acatttcagg atgttaggaa tgacccattt
    18841 ttcacagcaa gataattttt ctgttgcggc gcgcgtgttg ggcgcgctgt tttattacgc
    18901 tccagagagt gcggaagccg cacctctggt tgcggtactc accaatgaca gctgggaaac
    18961 tcagtggcct ctaccagaag cgtcattagc gccgctggtg acggcttttc agacacagag
    19021 tgaagagact cacgcccagg cctggcagcg tttgtttgtc ggcccgtggg cactgccgtc
    19081 tccgccgtgg ggatcggtct ggctggatcg cgaatctgtg ctgtttggcg attcaacatt
    19141 ggcacttcgt cagtggatgc gcgagaaagg cattcagttc gaaatgaagc aaaacgaacc
    19201 ggaagatcat tttggttcac tgctgctgat ggctgcgtgg ttggcggaga atggtcgcca
    19261 gacagaatgc gaagaattgc tggcatggca cctttttccg tggtcaacac gttttcttga
    19321 cgtttttatc gaaaaagcag aacacccttt ctaccgtgca ctgggtgaac tcgctcgctt
    19381 aacgctggcg caatggcaat cacaactgtt gattcctgtc gcggttaaac cgctgtttcg
    19441 ataggatcac cgtattattg ccggatggtg acgattacac gcaattcggc aattaatagg
    19501 ttacagtgtc acgttttttt atctcttaaa gcacgcactg cttttgcggc tggcctcttt
    19561 tgccgcaaaa tagtcgcccg tgtttcattg cccatttctg ctcatgcatc atctacacat
    19621 ctatccggat ctgcgcacta tgttccgccg tctgcttatc gcaacaatcg tcggtattct
    19681 tgcggccttt gccgttgccg ggtttcgtca tgcgatgctg ctactggagt ggctgttcct
    19741 caataatgac tccggcagtc tggtcaatgc agcgacaaat ctttccccgt ggcgacggct
    19801 gctaaccccg gcgctcggcg gactggcggc gggtttgttg ctgatgggct ggcagaaatt
    19861 tacccagcaa cgccctcatg cgccgaccga ttacatggaa gcgttgcaaa ccgatggtca
    19921 gtttgattac gcagcaagcc tggttaaatc gcttgcctct ctactggtag taaccagcgg
    19981 cagtgcaatt ggtcgcgaag gtgcgatgat tcttttagct gcccttgcaa cctcgtgttt
    20041 tgcccaacgt tttacgccac gccaggagtg gaaattatgg atcgcctgtg gagctgcggc
    20101 gggaatggct gcggcctatc gcgccccgct tgctggcagt ttatttatag ccgaagtgct
    20161 gtttggcact atgatgttgg cctctctcgg cccggtgatt atttccgccg tcgtggcgtt
    20221 gctgattagc aatcttatta atcatagcga cgcattactc tacagcgtac aactctcagt
    20281 gacggttcag gctcgtgact atgcgctgat tatcagtaca ggtgtgctgg caggtctgtg
    20341 cggaccactg ttgttaacgt taatgaacgc ctgtcatcgt ggatttgtga gtctcaagct
    20401 tgcgccgccc tggcaactgg cactgggcgg gttgaccgtg ggtctgcttt ccctgttcac
    20461 acctgcagtg tggggcaacg gctatagcac cgtacaatcc tttttaaccg ctccgccact
    20521 gttaatgatc attgccggga tcttcctctg taaactgtgt gccgtgctgg cgagtagtgg
    20581 ttctggcgcg cccggtggtg tctttacacc gacgctattt atcggtcttg ccattggcat
    20641 gttgtatggt cgtagcctgg gattatggtt acctgatggc gaagaaatta cgcttttact
    20701 cggattgacc gggatggcga cactgttggc ggcaacaacg cacgcgccga ttatgtcgac
    20761 gttgatgata tgtgaaatga ccggggagta tcagctactc cccggtttat tgattgcctg
    20821 cgtaattgcg tcggtaattt cgcggacgtt acaccgtgac tctatctacc gccagcacac
    20881 tgcgaagcat agctaagcgg atgtattgcc ccagttcacg ctgttcggcg cgcggcagat
    20941 aaggcagttc accaatgagc ggtgccggaa gttttttacc gagcacatca atgatttccg
    21001 cataatgcgc cagtcctggg ttgattcggt tagccaccca gccaatgagc ggcagcccgt
    21061 cgttggcaat cgcctgagct gttagcagtg catggttaat gcaaccttcc tgaataccga
    21121 caaccatcaa caccggcagt tgttcctgca ccacccattc agagagcgga cgcaaatcat
    21181 tcatcagact gcgccagccg ccagtccctt ctaccacgac atgatcgact ttatcggtca
    21241 ggtttgccag gccgtttgaa atgagggtgt aattgattgg gcaactgtgc gccacgctac
    21301 tttcttcttc gcttaacgcg ataggattaa ctgcttcata aggcagttcg atggttgaaa
    21361 cactctgcaa caccagggca tctttattac gcagcccttc gggtgtctct ttgctcccct
    21421 tcgctacggg tttatatccc gcaaccgttt ttccctggga ggctaacgct tgtagcaatg
    21481 cgcgggaaac caccgttttc cctacagaag tgtctgtacc ggtaataaag aaacgcttca
    21541 gcatcactaa ctccaccgtt atgcttcaca aatataaacc aggaaaataa ttaaccttga
    21601 aagtctaagt tatgctttcc tggcccaaat tgagatagcg caaattttgg tagaacagtt
    21661 aaaaaatgtt aaccctgcaa cagacgaatc aacaaagaac cgttatacat cgcgtctttt
    21721 accagcgcag cgcctgccat cgtgccctgg ttagaaaact gagtactctc aacgctaatg
    21781 tgctgactat acgcaggaag ggcctgctga cggatgctgt ccgagatgac tgggaagagg
    21841 atatctgccg ctttacttaa cggtgagcca atcagtattt tttgtgggtt aaataaattc
    21901 accatgatgg caagaatgcg accgacatgc gcgcctaccc cggtaatgat gtcttttgcc
    21961 agtagatcgc cgcgcaatgc cgcctgacac aatgagtcca cggttaacgg ttgtccatgt
    22021 aacatcgagc tcatggattg attgagacgc agctgtgcca gctcaagaat actgtccaca
    22081 ctggcgatgg tttcgaggca accgtgattc ccgcaataac agcgtttccc atacgggtcg
    22141 acctgtgtgt ggcctatttc cacgaggcta ctgctgcctg cgtgtagcag atgaccatcg
    22201 gtaatgacgc cagcacccac gttgtgatcg ataaccacct gaatcacatc gcgcgccccg
    22261 cgtgaggcac caaacaaggc ctcagccatc gtccatgcgc tgatatcatg ctgaatataa
    22321 accggtacgc cggtatgctg ctccagcgcc tcgccgagcg gcatctcttt tacatcctcg
    22381 tagaacggca tgcgatgtac aataccattt tccgtatcaa taattcccgg caaggttatg
    22441 gcaatcgaag tcagacgctc aagttttttc tggtggcgga taaaaaactg atcgatatgg
    22501 gaaatgatac gctccagcaa tggcgagtca tcttttaacg ccagttcctg cgactcttcc
    22561 actaccagtt tgctgctcag atcgcgcaaa gcaaggaaaa tctccccgcg actaatgcgc
    22621 agagaaagat agtgccaggc ttcagtttca accaccagcc ccactgccgg acggccacgg
    22681 ttccccgctt ctttgatttc cagctcttgc accaggtgtg cttcgagcat ctcacggaca
    22741 attttagtga tactggcagg agccagttgc gccagacggg aaagatcgat acgcgagact
    22801 ggaccaagct gatcaatcag gcgataaacc gcgcccgcgt tggtctgctt tatttgatca
    22861 atgtgcccag gctgattttc agcaaccacc gcatactccc tatattttcg cgctccgaaa
    22921 taatctgtag gctatggtga agcacttcaa tacgtgtcgt caaattttta cttaggcatg
    22981 tgattaacag cacatttttc gggctttttc gctgaaattt ccctcacctg agagcattca
    23041 gcagatgaat acgaaagtta cgcgcagccg gactttgttc atgatgtttc ggccagacca
    23101 accacatttc agaaaccgca tcctcttcag caatcggcac ccagcgcatt tcgttgagct
    23161 gaacacgttt aaatgacgca ggcaaaattg aaacacccag accggcggaa accagaccga
    23221 tgatggtcat tgcctcaccc acctcctgag tgatgacggg cgtcaaattg taacgtctca
    23281 tcagcccgag aatatcgtca tacagccctg ttccgacgtg cggatcaaaa aagacaaagg
    23341 gttctttcgc cagttcagcc agcgttacac tcgggttatt tgccagggga tgatcgtgcg
    23401 ggatcatcgc cataagcggt tcatggacga tgactgcgtg ttcaagcgtc tccggtaacg
    23461 ctgtgttacg cagcaatccc atatccagcg ttccttcaat gagcggagcg atttgctcgc
    23521 gagtgttcat ttcgcgggtt tgtaaatgga catcgggata atcacggcga aacagcgata
    23581 acgtatcgga caccgcccga ataaaaggag cggacgaagt aaaaccaatg cgcaactccc
    23641 ccgcttcgcc ctgatgcagc ctttcagcgc gagcggcagc atcatccacc atagacagga
    23701 tttgccgact atctacaaga aactgttttc ctgcttccgt cagcaataca ctgcgattgg
    23761 tccgtgccag cagtcgggca ccaatttgtt gctccagcgc ctgaatctgc tgacttagag
    23821 gtggttgcga aatattcagg cgggcagcgg cacgcccgaa atgcagctct tctgcaacag
    23881 cgacaaagta acgcagatga cgaagttcaa tattcatatt taaaacatct tatttgagat
    23941 tattaatata ttagacagaa caattcgatt ttcctaatct attcataagc ctgatccaca
    24001 ggcatattta gcaaggattt caagtgagcc gtactacaac tgttgatggc gctccggcaa
    24061 gcgacactga caagcaaagc atttctcagc caaatcaatt tattaaacgc ggtacgccgc
    24121 aatttatgcg cgtcaccctg gcgcttttct ctgccggact ggcaactttc gcacttctct
    24181 attgtgtgca acctatcctt ccggtgcttt cacaagagtt tggcttaacg cccgcgaaca
    24241 gtagtatttc actgtccatt tcaacggcga tgttggctat cggtttgctg tttactggcc
    24301 cgctatccga tgccattggt cgcaaaccag tgatggtcac ggcgctactg ttggcctcca
    24361 tttgtacgtt actttcgaca atgatgacca gctggcacgg cattttgatt atgcgcgcct
    24421 tgattgggct ttcgttaagt ggcgtggcag ccgttggcat gacttatctt agcgaggaaa
    24481 tccaccccag tttcgtggcc ttttcaatgg ggttgtatat cagcggcaac tcaattggcg
    24541 gcatgagcgg acgcttaatt agcggtgtct tcacggactt tttcaactgg cgaattgctc
    24601 tggcggcaat cggttgtttc gcgttggcct cggcgttgat gttctggaaa atcctccctg
    24661 aatcacgcca ttttcgcccg acttcgctgc gccctaagac gttattcatc aactttcgtc
    24721 tgcactggcg tgaccgggga ttaccgttat tgttcgcaga aggctttttg ctgatggggt
    24781 cgttcgtcac gctgtttaat tacatcggct ataggttgat gctttccccc tggcatgtca
    24841 gtcaggccgt ggttggctta ttatcgctgg cttatttgac cggtacatgg agctcaccca
    24901 aagccggaac catgaccacc cgctatggac gcggtccggt gatgttgttt tcgacggggg
    24961 ttatgctgtt tggtttactg atgaccttat tcagctcgct gtggctgatc tttgccggaa
    25021 tgttactctt ctcagccgga ttcttcgcag cccactccgt tgccagcagc tggatcggcc
    25081 cccgcgcaaa acgcgctaaa ggccaggcct cctcgctgta tctgttcagt tactatctgg
    25141 ggtcgagtat tgccgggacg ctgggtggtg ttttctggca taactatggc tggaacggcg
    25201 tcggcgcatt tattgctctg atgctggtca ttgctctgct ggtcgggacg cgtttgcatc
    25261 atcgtctgca cgcctgaaaa aataagtccg gactgaggta aatacccgtc cggacttatt
    25321 gcctgctcaa accaacgtta atagccatcc taaaatagac gaagcgccag ccaattcccg
    25381 cagcgcatct agcgtcatca ggattataag tacccaaata aacggattca ttttgctgtg
    25441 tgtcatttat tactgatgcg cagttattct accgctttgt aagtagtaaa atagttaaca
    25501 cgatcaagac tactattatt ggtagctaaa ttttccttaa gtcacaatac gttattatca
    25561 acgctgtaat ttattcagcg tttgtacata tcgttacacg ctgaaaccaa ccactcacgg
    25621 aagtctgcca ttcccaggga tatagttatt tcaacggccc cgcagtgggg ttaaatgaaa
    25681 aaacaaattg agggtatgac aatgaaaaaa gtattagctc tggttgttgc cgctgctatg
    25741 ggtctgtctt ctgctgcctt tgctgcagag actgcgacca cacctgctcc gactgcgacg
    25801 accaccaaag cagcgcctgc gaaaaccaca catcataaaa aacagcataa agcagcacct
    25861 gcccagaaag cgcaggcggc taaaaagcat cataaaaatg ccaaagctga acagaaagcc
    25921 cctgaacaaa aagcgcaggc ggcgaagaaa cacgccaaga aacacagcca tcagcaaccg
    25981 gcaaaacctg ctgcacaacc cgcagcgtaa gttttcaaca gtaatgctgg cgcgccccct
    26041 cgcgcctgaa aattacggtg ctaagcgggt aacgtttagc accgccttta gcggagggta
    26101 gtatggtggg ccgttatcgc tttgagttca ttcttatcat ccttatttta tgcgcactga
    26161 ttgccgcccg tttttatctt tcctgatcgt agttatctga ttttactccc actttcatcc
    26221 cgtcccgtct atagtattta tgagggtttg cttttaataa tcataattac ccaccagagt
    26281 gtgatatgcg tacaaccatt gctgtagtgt tgggtgcaat tagtttgacg tctgcttttg
    26341 tgtttgcaga taaaccagac gttgccaaat cggcaaacga tgaggtcagc accctgtttt
    26401 ttggtcatga tgatcgtgtg ccagtgaatg acacgaccca atcaccgtgg gatgcggttg
    26461 gccaactgga aacggccagc ggcaatttat gtacggcgac actgattgca cccaatctgg
    26521 cattaacggc aggacactgt ttattgacgc cgccaaaggg taaagccgat aaagcggtag
    26581 cgctgcgttt cgtgtcaaat aaaggtcttt ggcgctatga gatccacgac atagaaggcc
    26641 gcgttgatcc gacactggga aagcggttaa aagcagatgg ggatggctgg attgtacctc
    26701 ccgcagctgc gccctgggac ttcggtttga ttgtgctacg taatccccct tctggcatta
    26761 caccgttgcc gttatttgag ggagataaag ccgcgcttac cgccgcatta aaagcggctg
    26821 gtcgtaaagt gactcaggcg ggctaccctg aagatcatct cgatacgttg tacagtcatc
    26881 aaaactgtga agtgactggc tgggcgcaaa cgtcggtgat gtcgcatcag tgcgatacct
    26941 tgccgggtga cagcggttcg cctctgatgt tgcataccga tgacggctgg caattaattg
    27001 gggtgcaaag ctcggctcct gccgcgaaag atcgctggcg cgccgataac cgggccattt
    27061 ctgttaccgg ttttcgcgac aagctggatc aactgtcgca aaaataatgt tcaaacgctg
    27121 cccgacagcg cgggcagcgt gttcatcagg caagtttcac catgatcatt ccagccaaca
    27181 gcagcaccag gccaatccag cctttacgat ttaaccgttg accaaacaag atccaacctg
    27241 cggctaacgt ggcggcaata ccaaacccgc cccacaatgc ataagcgaca gacaagtcga
    27301 tccctttaac ggcctgagaa agcgcactaa aggcagccag caccgccgcc agggagagca
    27361 agccaaatat tttgcaacga aagccgtcag aaaatttcaa aaagacgtta gcaacgattt
    27421 ccagcacgat tgccaatgcc agccaggcgg cgtgaaccca ttcaaactgc gccatggttc
    27481 acctccagtt caggtttacg cgctttacgg gtacctgatt tgatcaatac aatcccggcg
    27541 accagggtgg ttaacccggc aattttcatc agcgataaac tttcgtcgaa taacaaaacg
    27601 ctaaacaagg taataaataa aataccgata ccttcccaca gcgcataagc cacgcctaag
    27661 gcgatttttt taacggcgaa agagagaaat atatacgaca gagaaatcat caccagcatt
    27721 aaaataaagc cgccatttcc ctcactgacg ctcgcccatt tcatggacag cgtaccggta
    27781 atttctgtag caatagccag acctaataaa atccaataaa tatacattgt ccttctcctg
    27841 caagagaatt attttaattt tcgcttaatt cagcgaaacc aaagtaaagt ggtcaagccc
    27901 cgcgcttagc gcaagctttg gcagaagaaa ggactaaagc gcgttgcgcc agtgctgctc
    27961 acctacgagc aagatagtgg atgaggtacg aagatgggta aatgtagaaa ataacgtcct
    28021 gaacaaattg tccataatat tacaattatc cgcagtgttg cttctcgtcc tcgcggatga
    28081 taattgtcct cggtagttga acacgcctga tttgtatcat agcttaagaa ttaactcaaa
    28141 atattttcac ttctttacct gagcggtttg attttcgtta tgatgacgga gcgaaaaaga
    28201 cattattatt agcaaaggaa gaaaaaacgg ggacaagcat ggcaaagccg atcatcacgc
    28261 tcaatggcct aaaaatcgtc attatgttgg gaatgctggt cattattctc tgcggtatcc
    28321 gttttgccgc cgagatcatc gtgccgttta ttctcgcatt atttattgct gttattctta
    28381 acccgctggt gcaacacatg gtccgctggc gtgtgccgcg tgtactggca gtgtcaattt
    28441 tgatgaccat catcgtgatg gcgatggtgt tgctgttagc ttatctgggt tccgcgctca
    28501 atgagttgac gcggacgtta ccgcaatatc gcaactctat tatgacgccg ctgcaagcga
    28561 ttgaaccgtt gttgcaacgc atagggattg atgtctcagt tgaccagctg gcgcattaca
    28621 ttgatccgaa cgcggcgatg acgttgctca ccaacttatt gacgcagtta tctaatgcca
    28681 tgtcatcaat ctttttattg ctgctgacgg tgctgtttat gttgctcgaa gtgccacaat
    28741 tgcccggaaa atttcagcaa atgatggcgc gtccggttga agggatggcg gcgattcagc
    28801 gtgcgattga cagtgtgtct cattatctgg tgctgaaaac agccatcagc atcatcaccg
    28861 gcctggtcgc ctgggcgatg ctcgccgcac tcgatgttcg cttcgctttt gtctggggat
    28921 tgctggcctt tgcgcttaat tacatcccga atattggttc agttctcgcg gcaatccccc
    28981 ctatcgctca ggtactggtg tttaatggct tctacgaagc gttgctggtg ctggcgggat
    29041 atctgctgat taatctggtc ttcggcaata ttctggagcc gcgcatcatg ggacgtgggc
    29101 tggggctttc cacattggtg gtatttttgt cgttgatttt ttggggatgg ttgttaggac
    29161 cggtgggtat gctgctttcc gtgccgttga caattattgt caaaattgcg cttgaacaaa
    29221 cagcgggagg tcaaagcatc gccgttctgt taagcgatct caataaagag tgacggcctc
    29281 cgcagaggcc gtcagggtta cagagccttc aggattgcat ccacgctggc tttggcgtca
    29341 ccaaacagca tgtgggtgtt ttccttgaag aacagcgggt tttgcacacc agcatagcca
    29401 gtgttcatcg aacgtttaaa gacaatcacg ttctgcgctt tccacacttc cagcacaggc
    29461 ataccagcaa tcggactctt cggatcatcc tgcgccgccg gattaaccgt gtcgttagca
    29521 ccaatcacca gtacggtatc ggtatcagca aagtcatcgt tgatctcgtc catttccagc
    29581 acgatgtcat acggtacttt tgcttcagcc agcaatacgt tcatatgtcc aggcaaacgc
    29641 cccgcaaccg ggtggatacc gaaacgcaca ttaataccac gagcgcgcaa tttctcagta
    29701 atttcagcga caggatattg cgcctgcgca actgccatgc cgtaccccgg agtaatgatc
    29761 actgaatggg agtttttcag cagttccgct gtctcttctg cagtgatttc gcggtgctcg
    29821 cccacttcct gatcatcacc agtagaagag ccgtcggtgc cgaaaccacc cgcaataacg
    29881 ctgataaagg aacggttcat cgccttacac ataatgtaag aaaggatagc ccccgaagaa
    29941 ccgaccagcg caccggtcac aatcagcagg tcgttgctga gcataaagcc cgcagccgca
    30001 gccgcccagc cggagtacga gttcagcatc gacaccacca ctggcatatc tgcaccaccg
    30061 atggaggcga ctaaatgcca gccgaatacc agcgcaatgg cggtcattat cagcaatgcc
    30121 agcacttgca ggccgacgct gtccgtgcga acaaatacaa tcagcagcag gaaggaaacg
    30181 accagagccg ccaggttcat tttgtgacgg tttggcagca tcaacggttt agacgaaatc
    30241 ttgccacaca gtttgccgaa cgccaccacc gaacccgtga acgttaccgc cccgatgaag
    30301 ataccgagga acacttccgt caggtgaata ttgaccagaa tcggtgccat tcccgcgtca
    30361 tgatgcagat agctgttaaa accaaccagc actgccgcca gacccacgaa gctatgcagg
    30421 atcgccacca gttctggcat ttcggtcatt tcaactttct tcgccagacg gataccaatt
    30481 gccccaccaa tgaccatagc cagcaagatc cagccaacat tacccgtatc cggtccaaaa
    30541 atggttgcga ttaacgcaat cgccatcccg gcgataccga agttgttacc ctggcgagac
    30601 gtttcatgtt tcgaaagacc ggccagactg aagataaaca ggatcgcggc aacaatgtat
    30661 gcagctgtaa ctaatcctcc agacatatgt taccccttaa tttttgcgga acattttcag
    30721 catgcgctga gtcacggtga agccaccgaa aatattaatg ctggctataa gcaccgcgat
    30781 aaaactaagg aagctaaccc agccgccctg gccaatctgc aacagtgctc cgacaacaat
    30841 aatccctgaa atcgcgttgg tgaccgacat caacggtgta tgcagcgcgt gcgatacatt
    30901 ccacaccacg taataaccga caacgcaggc cagcgcgaac acagtgaagt gtccaaggaa
    30961 ctcttttggc gcaacgcttg ccatccagcc gaaaaggatg attgccaacg ccatcaacgc
    31021 gtatttacgc cacggtgagc aggcacattt ttcctcagtt ttcacttccg gtgccgcttt
    31081 ttgtgccgcc tgcggctgag ctgatacctg aatcggcggt gccggccagg taatttcgcc
    31141 cgcacggatc acagtcacgc cacgaatcac cacatcatca aaatccacag tgatgttgcc
    31201 gtctttctct ttgcacaaca gtttcagcag attaacgagg ttagtgccgt aaagctgtga
    31261 ggattgcgtc ggcagacggc ccggaagatc ggtgtaacca attactttga caccattttc
    31321 cgtagtgaag atttcacccg gcacggtgta ttcacagttg ccgccgtttt gggctgccag
    31381 gtcgacaatc acactgcccg ccttcatgga gtcaaccatt tcacgggtaa ttagcttcgg
    31441 cgctggtttg cctggaataa gcgcggtggt gacaatgata tcgacctctt ttgcctgggc
    31501 ggcaaagagt tccatttccg ctttgatgaa cgcgtccgac atcactttgg catagccatc
    31561 gccgctgccc gcttcctctt taaaatccag ctcgaggaat tctgcgccca tactttgaac
    31621 ttgttctttc acttccgggc gggtgtcgaa tgcacgcaca atcgcgccga gactgtttgc
    31681 tgcgccaatg gcggccagac ctgcgacacc ggcaccaatc accatcactt ttgccggtgg
    31741 cactttcccg gccgcagtga tttgtccggt aaagaagcgc ccaaattcat gtgccgcttc
    31801 aacaatggcg cgataaccgg cgatattcgc catcgagctt agtgcgtcca gcgattgtgc
    31861 gcgtgagata cgcggcacag agtccatcgc catcacggtc acgttacgtt ccgcaagttt
    31921 ttgcattaat tccggattct gcgcaggcca gataaaactc accagcgttg tcccaggatt
    31981 cagtaacgca atttcatcat ctaacggcgc attgaccttc agaatgatct ctgactgcca
    32041 gacgctattc ccttctacaa tttcagcgcc cgcttgcaca aacgctttat cgtcaaaact
    32101 tgccagttga cccgcgccgc tctctaccgc gacggtaaaa cccagtttca gcagctgttc
    32161 cactgttttt ggcgttgctg caacacgggt ttcattggtt aaccgttctc ttggtattcc
    32221 aattcgcatg atattccctt ccatcggttt tattgatgat ggtttgcttg tgtcaggagc
    32281 cacacaagcc gctcatgtac gagctaaatg ttactccgtt aaaataaatt agtaacaaac
    32341 gccctataac gtactgaaaa ttatgcctgt gatctagcgc caaaaatcag tatttcggcg
    32401 tgaactcgca aaatattaac gattcagccg tgatagtggg ataaacacct tagaacgccg
    32461 gataaagact gataattgtc ttcggcggcc gggtaaaacg agacatcgcc ccggcacaaa
    32521 tcactactta acattaaatt aacttataca attcagttgc ttcagtagta ttgatggtga
    32581 tacggctgtt ttttaagcat agacggtcat ttgagcagaa ttaaaattgg tttaaggaat
    32641 gtgatatgaa aaatgacgca gacagttaca ccgtttaaat gcaataatca gccacgtttc
    32701 tcgttaataa caataccagt acctggtttg cgcaaggcga aggattattt ttatgaagct
    32761 taagaacacc ctcctggcgt cggcactgct ttctgctacg gcattctccg ttaacgcagc
    32821 aacagaactg acaccggagc aagcggcagc ggttaaacct tttgaccgtg tagtgattac
    32881 cggtcgtttt aatgctattg gcgaagcggt gaaagccgtt tctcgtcgcg cagataaaga
    32941 aggtgccgcc tctttttatg ttgtcgacac ttctgatttt ggtaacagcg gtaactggcg
    33001 tgtggtcgct gacctctata aagccgatgc tgaaaaagca gaagaaacaa gtaatcgcgt
    33061 aattaacggt gttgtcgaac tgccgaaaga tcaggctgtt ctgattgaac cgtttgacac
    33121 ggtcaccgtc cagggcttct atcgtagcca gccagaagtc aatgatgcca tcaccaaagc
    33181 agcaaaagcg aaaggtgcct actctttcta catcgttcgt caaatcgatg ccaaccaggg
    33241 cggcaaccag cgtattactg cattcatcta taaaaaagat gctaagaaac gtatcgtcca
    33301 gagcccggat gtcatcccgg cagattccga agcaggacgt gcagctctgg ctgccggtgg
    33361 cgaagctgcg aagaaagttg agatcccggg tgttgcgact accgcatcac caagttctga
    33421 agtcggtcgc ttctttgaaa cccagtcatc aaaaggcggg cgttacaccg tcacgctccc
    33481 ggatggcact aaagtcgaag aactgaacaa agcgacagca gcgatgatgg tcccgttcga
    33541 cagcatcaaa ttctctggca actacggcaa catgaccgaa gtctcctatc aggttgcgaa
    33601 acgtgccgcg aagaaaggtg ctaagtatta ccacatcacc cgccagtggc aggaacgtgg
    33661 taataacctg accgtcagcg cagatctgta taaataacta ttcagttact cctataaggc
    33721 ggcttgatgc cgcctttcgc atttttagtc ataaatttcc ctgcgaccat tgcatgcaga
    33781 cacatctctt cgtaaaatcc cgcgcttcag tggcaaactc catttttatg cgttttgccc
    33841 taatatttat tcttcttgtc acgttttaat cactggatat cgatggaaaa gaaactggga
    33901 ctgagcgcac tcaccgcgct ggtattaagc tcaatgctgg gcgcgggtgt tttcagtctg
    33961 ccgcaaaata tggcggcagt tgccagcccg gcagcactgc tcatcggctg gggtattact
    34021 ggcgctggca ttttattgct ggcctttgcc atgctgatcc tcacgcgcat tcgccccgaa
    34081 cttgacggcg gtatctttac ctatgcccgc gaagggtttg gcgagctaat cggcttttgt
    34141 tccgcatggg gatactggct gtgcgcggtc atcgccaacg tctcctatct ggtgatcgtt
    34201 ttttccgcgt taagcttttt tacggacacg ccggaattgc gcctgtttgg cgatggtaat
    34261 acctggcaat cgatcgtcgg tgcatcggct ttattgtgga ttgttcactt tttgatcctg
    34321 cgcggggtgc aaaccgctgc cagcattaac cttgtggcga cactggcaaa attgttgccg
    34381 ttgggtctgt ttgttgtgct ggcgatgatg atgttcaaac tggatacctt caagctcgac
    34441 ttcaccggac ttgcacttgg cgtacccgtg tgggaacagg tgaaaaacac catgctgatc
    34501 accctgtggg ttttcattgg tgtggaaggt gccgtggttg tttctgcgcg tgcgcgtaat
    34561 aagcgtgatg ttggcaaagc gacactgctg gcggttctct ccgctctggg cgtttatttg
    34621 ctggtaacgc tgctttcgct gggcgtggtg gcacgtcctg aactggctga aattcggaac
    34681 ccgtcaatgg ccggtctgat ggtggaaatg atgggaccgt ggggcgaaat catcatcgct
    34741 gccggtttga tcgtttccgt ttgcggcgcg tacttaagct ggaccatcat ggcagcagaa
    34801 gttccgttcc ttgcagccac tcataaagca ttcccgcgca tttttgcacg ccagaacgca
    34861 caagctgcgc catccgcttc actgtggctg accaacatct gcgtgcaaat ttgtctggtg
    34921 ctcatctggc tgaccggttc cgattacaac acattgctga ccatcgcctc agaaatgatt
    34981 ctggtgccct atttcctggt cggcgcgttc ttgctgaaaa tcgcaacccg tccactgcat
    35041 aaagcggtag gtgtcggtgc ctgcatttat ggcttatggt tattgtacgc ctctggtcca
    35101 atgcacttgc tgttatccgt tgttctgtat gcaccggggc tgctggtttt cctgtacgcg
    35161 cgtaaaacgc atacccatga taatgtactg aatcgtcagg aaatggtgct gattggcatg
    35221 ctgttaattg cctcagttcc ggcgacctgg atgctggtgg gataacgttc atcccatcgt
    35281 tggatagcaa aggagataac gatgggtaaa acccagtcct tgccaatatt aattactggc
    35341 ggaggtcgcc gcatcggcct cgccctcgca tggcatttca ttaatcaaaa gcaaccggtg
    35401 attgtcagct atcggacgca ctatcccgcc attgatggac tgattaaggc aggtgcgcag
    35461 tgtattcagg ctgatttttc gaccaacgac ggtgtgatgg cgtttgccga tgaagtacta
    35521 aaaagcaccc atggtctgcg tgctattttg cataacgcca gcgcgtggat ggcggaaaaa
    35581 ccgggagcgc cactgaccga cgtactggct tgcatgatgc agatccacgt taatacccca
    35641 tacctgctca accatgctct ggaaagatta ctgcgtgggc acggacacgc cgccagcgat
    35701 atcattcact ttaccgatta tgtggtggag cgcggtagcg acaaacatat tgcgtatgct
    35761 gcaagcaaag cggcactgga taatatgacc cgctcgtttg cccgcaagct ggcaccggaa
    35821 gtgaaagtga attctattgc gccatcgctg atcctgttta atgaacatga tgatgctgaa
    35881 tatcgacaac aggcgctgaa taaatcactg atgaaaaccg cgcctggcga gaaagaagtg
    35941 atcgacctgg tcgattactt acttaccagc tgctttgtca ccggacgcag tctcccactt
    36001 gatggcggtc gtcatctgcg ttaatgcagc ttaatccagc atataatcaa cacccatgcg
    36061 cttattcccc agcacgcaac agagccaaca aaagcagcgg gcaagcgcat cattccggtg
    36121 aaataccata gggacgccag gtaaacaaaa tagggaataa tcgaccacat actaaaaatg
    36181 atggttgcgc gtaaggcttc aatgccgcgt tcgctggcaa caatataatg cgcgataagc
    36241 gcaaaggtcg ggaaaagtgg aatcagcccg gcgatataat aatttttcgt ttttgctaaa
    36301 acaccaatca acagcactac cagcgcaccg agcgcggctt tgattaccag ccccatcttt
    36361 ttaccttaac actttcataa caagtcatca gtagaatacc tgatgaaacc ttgtttagaa
    36421 acgattgata gtaagtaaaa acagtgcggt gtattgtgac gtttttatat ctaccgtgaa
    36481 tgttatgaac actatcgtat ttgtggaaga tgatgcggaa gtcggtgcac tgattgccgc
    36541 atacctggca aaacatgata tgcaggttac cgtagagccg cgcggcgacc gggccgaaga
    36601 aactattctg cgagaaaatc cggatttggt gttactcgac atcatgctac caggcaagga
    36661 cggcatgacc atttgtcgtg atttacgcgc aaagtggtcc ggaccgattg ttcttctaac
    36721 ctctctcgat agcgatatga accacatcct ggcactggaa atgggtgcct gcgactatat
    36781 tctcaaaacg acgccccctg ctgttttgct agcgcgttta cgtttgcatt tgcgtcagaa
    36841 tgagcaagcc acagtgacca aaggtattca ggaaacgtct ctgactccct acaaagccct
    36901 gcatttcggc acgttgacca tcgatcccat caaccgcgta gtcaccctgg ctaacactga
    36961 aatctcgctc tcgacagctg atttcgaatt attgtgggaa ttagctaccc atgccgggca
    37021 aatcatggac cgcgatgcat tgctgaaaaa tttacgcggc gtcagttatg acggactgga
    37081 tcgtagcgtg gacgtggcta tttcgcggtt aagaaaaaaa ctgctcgata acgccgcaga
    37141 accttatcgt attaaaaccg tgcgtaacaa aggctatctt tttgcgcctc atgcgtggga
    37201 ataagcgatg aaaaaactgt ttatccagtt ttacctgtta ttgtttgtct gcttccttgt
    37261 gatgtctctg ctggtcgggc tggtgtacaa atttaccgcc gaacgcgcag gcaaacagtc
    37321 gctggatgat ttgatgaaca gttcgctgta tctgatgcgc agcgaattgc gtgagatccc
    37381 cccacacgac tggggtaaga cgctgaaaga gatggattta aatctctctt tcgatctgcg
    37441 tgtcgagccg ctgagtaaat atcatcttga tgatatttcc atgcaccggc tgcgtggcgg
    37501 cgaaattgtc gctctggacg atcagtacac ttttttgcaa cgtatcccgc gaagccacta
    37561 cgtgctggca gttggtcctg ttccttatct ttattacctc catcagatgc gattgctgga
    37621 tatcgccctg atcgctttta ttgccatttc cctcgccttt ccggtgttta tctggatgcg
    37681 tccgcactgg caggatatgt taaaactgga agcagcggcg caacgatttg gcgatgggca
    37741 tctcagtgag cgtatccact ttgatgaagg ttcgagcttt gaacgacttg gcgtcgcatt
    37801 taaccagatg gcggacaata tcaacgcctt gattgccagc aaaaaacagc ttattgacgg
    37861 tatcgctcac gaactgcgaa caccgttagt gcgcctgcgt tatcgactgg agatgagcga
    37921 taacctgagc gccgccgaat cccaggcgtt gaatcgcgat atcagtcaac ttgaagcttt
    37981 aattgaagag ctgctgactt atgcccgact cgatcgccca caaaatgagc ttcatcttag
    38041 cgagccagac ctgccgttgt ggctgtcaac gcatctggca gatattcagg cagtaacgcc
    38101 cgataaaacg gtacggataa aaacgctcat gcaaggccat tatgcggcgt tggatatgcg
    38161 cttaatggag cgcgtgctgg ataatttgct caataacgcc ctgcgctact gtcactcaac
    38221 ggttgaaacc agcctgctac tgtcggggaa tagagcgaca ttaattgttg aggatgatgg
    38281 cccagggatt gccccagaaa accgcgaaca tatctttgaa ccttttgttc gcctcgatcc
    38341 cagccgggat cgctcaaccg gcggctgcgg gctggggctg gccattgtcc actctatagc
    38401 actggcaatg ggcggtacgg ttaattgtga caccagcgaa ctgggtggtg cccgcttctc
    38461 gttcagctgg ccgttatggc ataacatccc gcaatttacc tctgcctgac actatgcgca
    38521 cggtggtcaa gtcaccatca ttgtgctata aaataaggat gttgtaacta aagtggttaa
    38581 tattatggcg cgttacgatc tcgtagaccg actcaacact acttttcgcc agatggaaca
    38641 agagctggct gcatttgccg ctcatcttga gcaacacaag ctattggttg cccgcgtgtt
    38701 ctctttgccg gaggtaaaaa aagaggatga gcataatccg cttaatcgta ttgaggtaaa
    38761 acaacatctc ggcaacgacg cgcaatcgct ggcgttgcgt cattttcgcc atttatttat
    38821 tcaacaacag tccgaaaatc gcagcagcaa ggccgctgtc cgtctgcctg gcgtgttgtg
    38881 ttaccaggtc gataactttt cgcaagcagc gttagtcagt catattcagc acatcaataa
    38941 actcaagacc acgttcgagc atatcgtcac ggttgaatca gaactcccct ccgcggcacg
    39001 ttttgaatgg gtgcatcgtc atttgccggg gctgattaca cttaatgctt accgcacgct
    39061 caccgttctg cacgaccccg ccactttacg ttttggctgg gctaataaac atatcattaa
    39121 aaatttgcat cgtgatgaag tcctggcaca gctggaaaaa agcctgaaat caccacgcag
    39181 tgtcgcaccg tggacgcgcg aggagtggca aagaaaactg gagcgagaat atcaggatat
    39241 cgctgccctg ccacagaacg cgaagttaaa aatcaaacgt ccggtgaagg tgcagccgat
    39301 tgcccgcgtc tggtacaaag gagatcaaaa acaagtacaa cacgcctgcc ctacgccgct
    39361 gattgcactg attaatcggg ataatggcgc gggcgtgcca gacgttggtg agttgttaaa
    39421 ttacgatgcc gacaatgtgc agcaccgtta taaaccacag gcgcagccgc ttcgtttaat
    39481 cattccacgg ctgcacctgt atgttgcaga ttaacccccg gctttcatac tgccgaccat
    39541 ctgttctggc cgtacccagc ggtcaaactc ggcttcgcta agatacccca gcgcaagggc
    39601 cgccgctttt aaggtcagcc cttctttatg cgcttttttg gcgatctcgg cggctttgtc
    39661 ataaccaatg tgggtgttaa gcgcagtcac cagcatcagc gattcattga gtaattgatt
    39721 gattcgctca cgattcggtt caatgcccac tgcacagtgt ttgttgaaac tttccatgcc
    39781 atctgccagc aagcgcaccg attgcaggaa attatggatc accatcggac ggaagacgtt
    39841 cagttcaaag ttaccggaag cgccacccat gttgatcgcc acgtcgttcc ccatcacctg
    39901 acagcagagc atggttaatg cttcgcactg tgttgggttc actttccctg gcatgattga
    39961 gctgcccggc tcattttccg ggattgagat ttcaccaatt ccgcagcgcg ggccagaggc
    40021 cagccagcgg acatcattgg caattttcat cagtgacgca gccaaccctt tcaatgcgcc
    40081 gtgcgcctga accagggcat cacaggtcgc cagcgcttca aatttgttcg gcgcggtaac
    40141 aaacggtgca caggtaatga ctgccagttc atctgctacg cgacgcgcat attccggatg
    40201 ggtatttagt ccagtaccca ccgctgtacc gcccagagcc agttccgcta cgtgaggcag
    40261 gctgtattcg atatgtttga gattatgctc cagcatcgct acccagccgg aaatctcctg
    40321 ccccagcgtt aacggcgtgg cgtcctgcaa gtgggtacga ccgattttga cgatatcggc
    40381 aaatgcgcgg gatttttcac tcagcgtctg ggtcagggtt ttaagttgtg gaatgagttg
    40441 cttgcgcagc gccagcagcg ccgcaacgtg catcgccgtc ggaaagacat cgttggaact
    40501 ttggcttttg ttcacgtcgt cgttagggtg aactttacgt tccatcccgc gcacgccgcc
    40561 gagtaattca ctggcccggt tagccagcac ttcgttcatg ttcatgttac tttgcgtgcc
    40621 ggagccggtc tgccagatag ccaacgggaa ttcgtcgtca tgctgtcctg ccagtacttc
    40681 atccgccgcc tgccgaatgg cgctcgcttt ctcttcagac aacaagccta aatcttcatt
    40741 aacttttgcc gctgcgcgct tggttagcgc cagcgcatga atcagtgagg tgggcatttt
    40801 ctccgtcgaa atgcggaaat gctccagcga gcgttgagtt tgtgcgcccc acagcttatc
    40861 tgccgggaca tcaatcgccc ccatcgaatc tttttcgctg cgtactgtat tcatggcctg
    40921 ctcctcacct gattaatttt ttctttctgt tttgctttcg ttaagcaact tttagcttac
    40981 tcattattta ccatttgata acaaatgttt ggtctttcgt gccatgtaaa aaaaccgccc
    41041 cgaagggcgg ctctgtttat ttcacacagc gggtgcattg tgtgagttgt atctgctgga
    41101 agaagtcatt tcctttatca tccacaagga taaacgccgg gaaatcttcc acttcaattt
    41161 tccagatggc ttccattccc agttccggat attcaacaca ttccaggctc ttaatacttc
    41221 cctgcgccaa tacagctgcc ggaccaccga tactgccgag gtagaagccg ccgtgttttt
    41281 tacaggcatc cgtcacctgc tggctgcggt tgcctttcgc cagcatgatc atacttccgc
    41341 cctgcgcttg cagttgatcg acataagaat ccatccgtcc ggcggtcgtt gggccaagag
    41401 aaccggaggc ataaccttcc ggcgttttgg ccggacccgc gtagtaaatc ggatgatctt
    41461 taatgtactg cggcagccct tcaccgttat ccatccgctc tttcagtttg gcgtgagcaa
    41521 tatcacgacc gacgataatc gtgccgttaa gcgataagcg tgtagaaacg ggatactgcg
    41581 acaactgtgc gaggatctct ttcatcggac ggttaaggtc aacgcgcacg gcttcgcctt
    41641 ctcctgcttt gcgcagctct tccgggatat atttgcctgg attatgttcc agtttttcga
    41701 tccagatccc ctgacggttg atcttcgctt tgatattacg gtcagcagaa caggagacgc
    41761 ccataccgac cgggcaggat gcgccgtgac gtggcaggcg aatcacgcgg atgtcatgag
    41821 cgaagtattt accaccaaac tgcgcaccca gaccaagatt ttgcgcttcg atcagcaatt
    41881 ctttttccag ttccacatcg cggaacgcct gaccgtgctc attcccttcc gttggcagtt
    41941 catcatagta tttagcggaa gcgagtttca ccgttttaag attcgtttct gcagaagttc
    42001 caccaataac gaacgcaata tgatacggag gacaggccgc cgtacccagc gtgcgcatct
    42061 tctcaaccag gtaatttttc agttttcccg gcgtcagtaa cgctttggtt tcctgataga
    42121 gatacgtctt gtttgccgaa ccgccacctt tggcgataca gaggaatttg tactcgtcgc
    42181 catcaacggc ataaagatcg atctgcgctg gcaagttggt gccggtattc acctctttat
    42241 acatatccag cggcgcgttt tgcgagtagc gcagattatc ctcgatataa gtgttataga
    42301 caccgcgcgc cagcgccgct tcatcaccgc caccggtcca tacacgctgc ccttttttac
    42361 caacaataat cgcggtgccg gtatcctgac aggttggcag aacgcctttt gccgcgatgt
    42421 cggagttacg caggaattgc agcgccacat atttatcatt ttcgctggcc tccgggtcac
    42481 gcagaatgtc ggccacctgt tgttggtgag ccggacgcag cataaatgac gcatcatgaa
    42541 acgcctgacg cgccaacaga gttaacgctt cgggtgcgac tttcaaaatc tcctgccctt
    42601 caaattcaga tacgctaacg tgttcgctgg ttagcaggta atactcagta tcatcttttt
    42661 tgagtggaaa aggagcctga taatgaaagg gtttgtttga cattgttctc tcacttactg
    42721 cctggtttgg ttatgctctg ggcgggtgtt ccgttgccct gttaaaagcg agtaacaata
    42781 tcctacacac ttttttaaca aaaactgaga ctagtacgac tttttgcggc tccaggttac
    42841 ttcccgtagg attcttgctt taatagcggg attaatttcc acattaaaac agggattgat
    42901 catgcaaaaa ctcattaact cagtgcaaaa ctatgcctgg ggcagcaaaa cggcgttgac
    42961 tgaactttat ggtatggaaa atccgtccag ccagccgatg gccgagttgt ggatgggcgc
    43021 gcatccaaag agcagttcac gtgtgcacga tgccgcagga gatatcgttt cactgcgtga
    43081 tgtgattgag agtgataaag cgactctttt gggagatgcc gttgccaaac gctttggcga
    43141 gttgcctttc ctgttcaaag tattatgcgc agcccagccg ctctccattc aggttcatcc
    43201 aaacaaacgc aattctgaaa tcggttttgc caaagaaaat gctgcaggta ttccgatgga
    43261 tgccgctgag cgtaactata aagatcctaa tcacaagcct gagctggttt tcgccctgac
    43321 ccctttcctc gcgatgaacg cgtttcgtga gttttccgag attgtctccc tgctccagcc
    43381 agtcgcaggt gcacatccgg cgattgctca ctttttacaa cagcctgatg ccgaacgttt
    43441 aagcgaactg ttcgccagcc tgttgaatat gcagggggaa gaaaaatccc gcgcgctggc
    43501 gattttaaaa tcggcccttg acagccagca gggtgaaccg tggcaaacga ttcgtttaat
    43561 ttctgaattt tacccggatg acagcggtct gttctccccg ctgttgctga atgtggtgaa
    43621 attgaatcct ggcgaagcga tgttcctgtt cgctgaaacg ccgcacgctt acctgcaagg
    43681 cgtggcgctg gaagtgatgg cgaactccga taacgtgctg cgtgcgggtc tgacgcctaa
    43741 atatattgat attccggaac tggttgccaa tgtgaagttc gaagccaaac cggctaacca
    43801 gttgttgacc cagccggtga aacaaggtgc agaactggac ttcccgattc ctgtggatga
    43861 ttttgccttc tcgttgcatg accttagtga taaagaaacc accatcagcc agcagagtgc
    43921 cgccattttg ttctgcgtcg aaggcgatgc aacgctgtgt aaaggttctc agcagttaca
    43981 gcttaaaccg ggtgaatcag cgtttattgc cgccaacgaa tcaccggtga ctgtcagagg
    44041 ccacggccgt ttagcgcgtg tttacaacaa gctgtaagag cttactgaaa aaattaacat
    44101 ctcttgctaa gcttattaaa ggcttataac accttcaggc ggccagtccg cctgatttca
    44161 ttttatggat aatcattatg aataaatcgc tggtagcggt aggcgtcatt gttgcgctag
    44221 gcgtagtctg gacaggcggc gcatggtata caggcaagaa gattgaaacc catctcgaag
    44281 acatggtcgc gcaggcgaac gcgcaactca aactgacagc tcctgaatcc aacctggaag
    44341 tcagttatca aaactatcat cgcggcgtat tcagcagcca gttgcaactg ttggtgaaac
    44401 ccattgccgg aaaagtaaat ccgtggatta aaagcggtca gagcgtcatc ttcaacgaat
    44461 cggttgatca tggtcccttc ccgcttgccc agcttaaaaa actgaacctg atcccgtcga
    44521 tggcatcaat tcaaaccacg ctggttaata acgaagtaag caaaccactg tttgatatgg
    44581 caaaaggtga aacgcctttt gagattaact cccgcattgg ttacagcgga gattccagtt
    44641 ccgacatttc gctcaagcca ctgaattacg agcaaaagga tgaaaaagtc gcctttagcg
    44701 gcggcgagtt ccagttaaat gcagacagag acggcaaagc tatctccctt tccggggagg
    44761 cgcaaagtgg tcggatagac gcggttaacg aatacaacca gaaagtgcag ttaaccttta
    44821 ataatctgaa aaccgacggt tccagcacgc tggcaagttt tggtgagcgc gtaggaaacc
    44881 aaaaactatc gctagaaaaa atgaccattt cagtggaggg caaagaactg gcactgctgg
    44941 aaggcatgga gatcagcggt aaatcggatc tggtcaatga cggtaaaacg atcaatagcc
    45001 aactggatta ctcgctaaac agcctgaagg tacagaatca ggatctgggc agtggcaagc
    45061 tgactttaaa agtcggccaa attgatggcg aagcctggca tcagtttagc cagcaatata
    45121 acgcgcaaac tcaggcgctg ctggcacagc cagaaattgc caacaatccc gaactttatc
    45181 aggagaaagt gacggaagcc ttctttagcg ctctgccgct gatgctgaaa ggcgatccgg
    45241 tgattactat cgcgccgcta agctggaaaa acagtcaggg tgaaagtgcg ctgaatctgt
    45301 cgctgttcct gaaagatccg gcaacgacta aagaagcgcc gcaaacgctg gcgcaggaag
    45361 tggaccgctc ggttaaatct ctggatgcga aactgaccat tccggtggat atggcaactg
    45421 agttgatgac tcaggtagcg aagctggaag gttatcagga agatcaagcg aaaaaactgg
    45481 cgaaacagca agttgaaggt gcatcagcaa tggggcagat gttccgtctg accaccttgc
    45541 aggacaatac catcaccacc agcctgcaat atgctaacgg tcagataacg ttaaacgggc
    45601 agaaaatgcc actggaagat ttcgtgggta tgtttgcgat gccggcatta aacgttccgg
    45661 ctgtacccgc tattccgcag cagtaattca cagccaaaac gccggagatt tttctctccg
    45721 gcgttttttc ttacttcagc ataaagtcat actgaatact cattttcaca tcccagtaat
    45781 tcaggcgatc gcccagcgta ctgccatcgc gcttttcccg ccctttatcg gcagcaagaa
    45841 agaaattcat cccttgtaac ggcccttcgc tgacccgata gttcataccc cctaagatac
    45901 ccgcgccttc aatccgcaca tctcgtttac tggattcata gccgcgggtc acaatcggtg
    45961 caaatgtcag cgtaaattgc ggagttaaac gatagttgac gcccaattgc caggactgca
    46021 ttccttcacg gttacgatca agacttaatg aaaagggata gccaatgtcg gtatccacca
    46081 cggcaccatt cattgcatgg cgcaaataac caccactgcc aaatacgctt ccccacggcg
    46141 cattccaggt caattgtcca ctaaccaacg ccgtttcatt gggctggctg gtattacgac
    46201 ttaaaccttc aagttgcgcg taaaagccga gcaactcccc tttcaacgta taattcgggt
    46261 caagcggtat gcttttctcg agaatacttt gtatctgatt ggaaacatct ttctgtaccg
    46321 atgcccccag agaatagcga ctggtatcat catgccaact ggcggctaaa aaagaacgtg
    46381 cgcgatgttt atcgtatttt cccgtctggc tgttataaac cccttcatca cgaataccgg
    46441 ttgttgaatc ggtccataaa aatactcgtg aacgccacat tccccacaga tcaatatttg
    46501 gcgttaaagc ccaataacct aatgctcctt cagaaacagt cggtaatgta cgttgcgaac
    46561 tgccagccat taacggaata cgataaggta agctaccata actataatcc gtcccgaagc
    46621 gacctaattt tagcaggcta ttttcactgg gttttatttt tactgccccc agggcaaagc
    46681 caaaactttt atcaccatca agataccacc gggtactcat atcagcacga gcacctaatt
    46741 tataaacata atacgccccg ccttcaacgc caataatatt ggaataataa ccactattga
    46801 aatcgagcaa tcctccctgt acccatgcgt aaatatctct tcccgtgcca gcactgggct
    46861 tatcagccct gcgcaattcg tttttcagtc tgatgcgtag tcccgcttca tcgtcagcca
    46921 gttgcgccgc gtaagcagag gtaaggccag aggcagccgt caggcaaata acggccatgg
    46981 ccactatttt tctcatagtt gcacctttga tgttctgatt atttttattg aatattaact
    47041 agtgatatcg ttgattaatt gctgctgaac ttttttacga ttatcaattt caacaacgat
    47101 ttctttgaat tttttatccg tgagcggata aaaccagata atgacgaatg ccagtagcat
    47161 aaatccgcaa ggtactaagg caattgatgt gcggatgccc ataataactt ccggcgtttg
    47221 cgcctgattg gcgatatatc cgcttaatcc caaaataaag gcaggaattg aacctccgat
    47281 tgcctgaccg catttacggg taaatgagaa tagtgaatag gtgagccctt caattcgcac
    47341 gccggtcagg tattcaccgt attctacggt atcagcttcc agtgcccaca tcacggtcat
    47401 ggtaacgccc tggccaattg atgcgatggc caacgcaacc aacgccaccg gcagtgacca
    47461 gacggaaacc cagaagaaca gcagataacc gcaggttccc agcaaagcgc caatcaggaa
    47521 ggtattcttt ttaccgatcc tcgcgaccat ccccggcacc agcggtgccg atgccacagt
    47581 accgaccagg ttttgcacca gtaccagcac agtgaacagc ccggtatcat ttaacacata
    47641 gcgcacgtag aacaacgacg aggcgctgac cgcgaaggtc gaaatcagca cacacagcgc
    47701 accgatgcac aacataaaca gcgggcgatt ccgtttcagg gtttgcagac tgatcttcaa
    47761 tgacggctgc gccacgatac gtaccacatt ctcacgcgtc gatttgaagc agatgaagta
    47821 aagcaccatt ccggcaatcg ccagcacaat tgtccagaaa tgatataccg acaccatctc
    47881 ttccgggctg gagttcttaa tgctcggtcc tatcagaaat gccaggcaga caaaagtcaa
    47941 tgaagcggca atcccgcgag ccgcgcccag acgggcgcgg gattgtggtt gttgggtcat
    48001 cgcggtagca agtgaaccat aaggaatatt caccaggctg tagcaaagcc cgagtcccat
    48061 gtaggtcaaa tatgcataca caactttgct gctatggctc cagtcggtcg gcacccagaa
    48121 taccagcacg ctgaagatca ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt
    48181 tccccatcgg gtattcacac tgtccaccac tcgtccggca aagacatcgg cgaaggcatc
    48241 gaatacccgc accagtaaca gcatggtgcc cgccgcagcg gcaccgacgc cagcgacgtc
    48301 ggtgtagtaa ctcaacagga agagcgcccc cattgcgaag gcgaagttat tggcgacgtc
    48361 accgaggctg tagccgacga tggtgcgcca ggagagttgt tgattcattg tttgcctccc
    48421 tgctgcggtt tttcaccgaa gttcatgccg gtccagcgtt tttgcagcag aaaagccgcc
    48481 gacttcggtt tgcggtcgcg ggtgaagatc cctttcttgt taccgccaac gcgcaatatg
    48541 ccttgcgagg tcgcaaaatc ggcgaaattc catacctgct caccgacgac agcgctgacg
    48601 cgatcaaaga cgcggtgata catatccagc catgcgcact gatactcttc actccacatg
    48661 tcggtgtaca ttgagtgcag gccggctaac gtatccacac cgtattcggt gatgataatc
    48721 ggctgatgca gtttctcctg ccaggccaga agttcttttt ccagaacctt ctctgccgtt
    48781 tccaaatcgc cgctttggac ataccaaccg taataacggt tcaggcacag cacatcaaag
    48841 agatcgctga tggtatcggt gtgagcgtcg cagaacatta cattgacaca ggtgatcgga
    48901 cgcgtcgggt cgagtttacg cgttgcttcc gccagtggcg caaaatattc ccgtgcacct
    48961 tgcggacggg tatccggttc gttggcaata ctccacatca ccacgcttgg gtggtttttg
    49021 tcacgcgcaa tcagctcttt aatcgcctgt aagtgcgcct gctgggtttc cccgttgact
    49081 gcctcttcgc tgtacagttc tttcggcttg ttgcccgctt cgaaaccaat gcctaaagag
    49141 aggttaaagc cgacagctgc agtttcatca atcaccacga tgccatgttc atctgcccag
    49201 tcgagcatct cttcagcgta agggtaatgc gaggtacggt aggagttggc cccaatccag
    49261 tccattaatg cgtgatcgtg caccatcaac acgttatcga atcctttgcc gcgcaaatcc
    49321 gcatcttcat gacgaccaaa gccagtaaag tagaacggtt tgtggttgat caggaactgt
    49381 tcgcccttca ctgccactga ccggataccg acgcgcagcg ggtagatatc acactctgtc
    49441 tggcttttgg ctgtgacgca cagttcatag agataacctt cacccggttg ccagaggtgc
    49501 ggattcacca cttgcaaagt cccgcaggtg ccttgtccag ttgcaaccac ctgctgatcc
    49561 gcatcacgca gttcaacgct gacatcacca tttgccccca cctgccagtc aacagatgcg
    49621 tggttacagt cttgcgcgac atgcgtcacc acggtgatat cgtccaccca ggtattcggc
    49681 gtggtgtaga gcattacgct gcgatggatc ccggcgtagt taaagaaatc atgaaagtaa
    49741 gactgctttt tcttgccgtt ttcgtcggta atcaccattc ccggcgggat agtctgccag
    49801 ttcagttcgt tgttcacaca aacagtgata cgtacacttt tcccggcaat aacatacgga
    49861 gtgacatcgg cttcaaatgg cgtatagccg ccctgatgct ccatcacttc ctgattattt
    49921 acccacactt tgccgtaatg agtgaccgca tcgaaacgca gtacaatacg ctggcccgcc
    49981 caacctttcg gtataaagac ttcgcgctga taccagacgt tgcccacata attacgaata
    50041 tctgcatcgg cgaactgatc gttaaaactg cccggcacag caattgcccg gctttcttgt
    50101 aacgcgcttt cccaccaacg ctgatcaatt ccacagtttt cgcgatccag actgaatgcc
    50161 cacaggccgt cgagtttttt gatttcacgg gttggggttt ctacaggacg taacataagg
    50221 gactcctcat taagataaaa atactggtca acctttaatc tgattagtta accaattctg
    50281 tgttgcacac agttatctgt gagaaaccca tggaagagaa atggaaataa aaagaactga
    50341 caccgcgatc aaaaaaccaa aaactgcaat acccacagcg tgatattgat cgcattaatc
    50401 tttaaaacag cgttgacaga agccacaaaa taagcaatcg aacgttcgct cgaacatatt
    50461 ggtagcgata agccctcatg atatattgct acgatgaata tttaggtggg attctccgat
    50521 ccctgggagg atgatttttg cacagagaaa ttacgatatg aaacgcgggc acagttgtca
    50581 ccagcccgcg gaaagagaga gaggatcagg atgcggttaa gataccgcca atcatcgcgc
    50641 gtaaaccctg agcaaacgac gcttcacgcg ccaggtcttc cgcttcgata tcggccagta
    50701 caccgtaggt cagatccagc agtaaacgtg aagtcattgc cgtgttgatg tctgggctta
    50761 tctcgccttt ttgctgggca tcacgcatcc gctgggcaac aaattccgtg atcgtcatat
    50821 gcttattttt aagcatggcg gcgacctgtg ggttacgcat cccttccgcc ataatttcga
    50881 ccaccagcgc tcgttgccca aagacttcat gggtgagaga gacaatagac tcgaccatat
    50941 agtcaacgaa atgaatccct tcaatcggtt cccggaaacg ggccagcgcc ctctcctggt
    51001 cctgtaagat aatcgcctga atcaaggctt ctttggagat gaaatggtga tagagcgtcc
    51061 cgggactaat ggcgcaggat ttacagatgg ctttcatcga ggcactgtga aatccatttt
    51121 ctgaaaaaat ctctctggca gcattgagga tccgggtccg tgtcggttgt gcttcagtct
    51181 gcatgttatc catcattaaa ccattatagt tagcatgaat aactagttta ccgcgttgtc
    51241 aggtgagcgc ctatcattgc tgttgaatag tgcgaaggca cactcaattc gtaagctttt
    51301 tatccagata ttggttaacc aatagccgtg atttggctgc cagttgttta gcagagacgt
    51361 tgatgagttg cacacgctat cccggtaaac aacacatacg gctgtttacc ggttcgttag
    51421 tgtattaatt gagctcctgt accccaccac cggagacggt gagaatttgt ccgcttaccc
    51481 agctggctgc aggcgagcaa aggaacagcg ccgcgttagc tatatcttgc ggttggccca
    51541 gacgtctgat tggtgtgtgt tgcaacattt tctgttcaat ttctggtgta ataacggatt
    51601 tcagggcatc ggttaatatt gcccccggtg caatgccatt cacccgaata tttttttcac
    51661 ccaggtcaaa cgccatattt ctgaccagat gactggccgc agctttagat gatgcatagg
    51721 aggtcatgtt tatattttta ttttctgccg ccatagaagt aatggtcaga ataacgccac
    51781 cgccattttt ttccatttct ggcgcaacaa gttgtgacag atggaaaaaa gaaaatacat
    51841 tcagttcata agcgcggcga aaatctgcca ttggcatatc aaacggttta ggcccaccgc
    51901 caccggcgtt gttaaccagg atatcaactt tacccaactt actgacggca aagtctgcca
    51961 gtgcagagag ttcctgttcg gaagtaatat cgcagcggca ggcaaatgcc tgaccaccca
    52021 gttgttgaat ttcatttaca acatgattag ctgcatcggc gttaatatca ctgaccacca
    52081 cagatgcgcc agctgtcgcg aatgtaatgg cgatttcttt accaatacct gcacccgcac
    52141 ctgtgatgat ggcgcatttt ccgtcgagtc tcaggttgtc agaattaaac acataaacct
    52201 cctgtgaact tcagtaaaaa taaaaggcac cgcctgtgtg ccacattttt tatcattaac
    52261 cttgaggaaa ggacactacc aggtatagca gataaagaga ggattatttc gccgcaatga
    52321 gccgggcggg aataataaga ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca
    52381 tgcgatccgc aagtgtggta ccaagttccc gcgctggcgt gctggcccag gtaacgggta
    52441 tatcatcaag tgtagttggc gttgcatcgg taaatgccgc cagcgaaacc tgttgctcaa
    52501 agtaacgatc gactccgctt tccccgcttt gccttcctgc tttcagcaaa ccaaaccatg
    52561 cccccatcgc aatagtttcg ttatagcaaa ccacggcgct gatggtcggg ttatgacgta
    52621 aaagcgccgt gatagcttcc gcggcttgct tctggctgga agtgcactcc agcacccaat
    52681 cgctgtgaaa cggcaggcca aattttagta gagtcgcaca atagcccccc acccgttctg
    52741 cccgggttaa tgaggaactt tgccctccca gccaggcgat acgctgatgc ccattgcgaa
    52801 tgagatgctc cgtcaacaac tgtgcagcct gcatgttgtc cgggcgaacc gtatcaacgt
    52861 catcaagata actggcacgg gaagcgaaaa tcaccgggat agctttttct tctgctatcc
    52921 gtcgcaggtc atcgctgctt cctgcagccc cggcaatgac cacaccatcg acaccctgat
    52981 tcagtaatag tgaaaaccgc tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa
    53041 aaaccatccg tccctgcgct tccagagctt ccgtcaatcc ggccgtcaat tcggcgtaaa
    53101 acggcgcaga taaatcacgg acgatcaaac caatgacgcc actttgcccg ccgcgcagcg
    53161 ccgacgcctg ccgattgcgc acaaatccca gctcttcaat ggcggcgtta acgcgttctc
    53221 ctgtggcggt agagattcgc cctttgccac ttagcaccag cgaaacggtg cttaccgaca
    53281 cgcccgcagc cagcgcaaca tcatgaatgg ttattttttt ggcggtagcc atgcagatga
    53341 cctactccct gattatgtga cagataaaac gttttacctt ttatttatct tatacccgct
    53401 attatcgttg cgtaatgtga tttatgcctc actaaaattt gataaaacgt tttatcttct
    53461 cgcgcaattt actgaatcca gattgttctc tacgaggagt cgttttatga cggcgaaaac
    53521 agcaccgaaa gtcacgctgt gggagttctt ccagcagtta ggcaaaacct tcatgttacc
    53581 cgtggcatta ttgtcgttct gcggcattat gctcggcatt ggtagttctc ttagcagtca
    53641 tgatgtcatc accctgatcc cggtcctggg caaccccgtg ttgcaggcta tctttacctg
    53701 gatgagtaag attggctcgt ttgcttttag tttcctgcca gtcatgttct gtatcgccat
    53761 cccgctgggt ctggcacgcg aaaacaaagg cgtagcggca ttcgctggct tcgtcggtta
    53821 tgcggtaatg aacctcgcgg taaacttctg gttgaccaat aaaggcattc tgccaaccac
    53881 ggacgccgcg gttctgaaag ccaataacat ccagagcatt cttgggatcc agtcgatcga
    53941 caccgggatc ctcggtgcgg tgatcgccgg tattatcgtc tggatgctgc atgagcgatt
    54001 ccataatatc cgcctgccag atgcgctggc attcttcggc ggtacgcgct tcgtaccaat
    54061 catctcctcg ctggtgatgg gtcttgtcgg cctggtgatt ccattagtct ggccaatttt
    54121 cgccatgggt attagcggct taggccatat gatcaacagc gcgggagatt tcggaccgat
    54181 gctgtttggt accggtgaac gtctgctgtt gccgtttggt ctgcatcaca ttctggtggc
    54241 attaattcgc tttaccgacg caggcggcac gcaggaagtc tgcggtcaaa ccgtcagcgg
    54301 tgcactgacc atcttccagg cgcaattgag ttgcccaacc actcacggtt tttctgaaag
    54361 cgccacgcgt ttcctttcgc aaggtaaaat gcctgcgttt ctcggcggtc tgccaggtgc
    54421 agcgttagct atgtatcact gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct
    54481 gatttctggc ctgatcgcct gtgtcgttgg cggcacgacc gaaccgctgg aattcctgtt
    54541 cctgttcgta gcgccagttc tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac
    54601 tgtcatgtct gtgctcggcg tcaccatcgg taataccgac ggcaatatca tcgactttgt
    54661 ggtgttcggt attttgcatg gtctgtcaac caagtggtac atggtgccag tagtggcggc
    54721 aatctggttt gtcgtttact acatcatctt ccgtttcgct atcacccgct tcaatctcaa
    54781 aaccccgggg cgcgatagtg aagttgccag ttcaatcgaa aaagccgttg ccggtgcgcc
    54841 gggtaaatca ggttacaacg ttccggcaat cctcgaagcc ttaggcggtg ccgacaatat
    54901 tgtcagtctc gataactgca ttacgcgtct gcgtttgtct gtaaaagata tgtcgcttgt
    54961 taatgtgcag gcactgaagg acaatcgggc aattggcgta gtacaactta atcaacataa
    55021 cctgcaggtt gttatcgggc cacaagttca gtcagtaaaa gatgaaatgg tcggtctgat
    55081 gcgtactgtc caggcataag gataagatat gttcgatttt tcaaaggtcg tggatcgtca
    55141 tggcacatgg tgtacgcagt gggattatgt cgctgaccgt ttcggcactg ctgacctgtt
    55201 accgttcacg atttcagaca tggattttgc cactgccccc tgcattatcg aggcgctgaa
    55261 tcagcgcctg atacacggcg tgtttggcta cagccgctgg aaaaacgatg agtttctcgc
    55321 ggctattgcc cactggtttt ccacccagca ttacaccgcc atcgatcctc agtcggtggt
    55381 gtatggccct tctgttatct atatggtttc agaactgatt cgtcagtggt ctgaaacagg
    55441 tgaaggcgtg gtgatccaca cacccgccta tgacgcattt tacaaggcca ttgaaggtaa
    55501 ccagcgcaca gtaatgcccg ttgctttaga gaagcaggct gacggctggt tttgcgatat
    55561 gggcaagctg gaagccgtgc tggcgaaacc agaatgtaaa attatgctcc tgtgtagccc
    55621 acagaatcct accgggaaag tgtggacgtg cgatgagctg gagatcatgg ctgacctgtg
    55681 cgagcgtcat ggtgtgcggg ttatttccga tgaaatccat atggatatgg tttggggcga
    55741 gcagccgcat attccctgga gtaatgtggc gcgcggagac tgggcgttgc taacgtcggg
    55801 atcaaaaagt ttcaatattc ccgccctgac cggtgcttac gggattatag aaaatagcag
    55861 tagccgcgat gcctatttat cggcactgaa aggacgtgat gggctttctt ccccttcggt
    55921 actggcgtta actgctcata tcgccgccta tcagcaaggc gcgccatggc tggatgcctt
    55981 acgcgtctat ctgaaagata acctgacgta tatcgcagat aaaatgaacg ctgcgtttcc
    56041 tgaactcaac tggcagatcc cgcaatccac ttatctggcc tggcttgatc tacgtccgtt
    56101 gaatattgac gacaacgcgt tgcaaaaagc gcttatcgag caagaaaaga tcgcgatcat
    56161 gccgggatat acctacggtg aagaaggtcg tggttttatc cgactcaatg ccggctgccc
    56221 acgttcgaaa ctggaaaaag gtgtggctgg attaattaac gccatccgcg ctgttggtta
    56281 accccaattg cgcaacgtaa aaaatcgttg cgcaatcgtg gatttttacc ctgctttgtt
    56341 tttataatgg tgcgcacttt tatatccaga aaaagagtgc gaccatgatt gataccaccc
    56401 tgccattaac tgatatccat cgccaccttg atggcaacat tcgtccccag accattcttg
    56461 aacttggccg ccagtataat atctcgcttc ctgcacaatc cctggaaaca ctgattcccc
    56521 acgttcaggt cattgccaac gaacccgatc tggtgagctt tctgactaaa cttgactggg
    56581 gcgttaaagt tctcgcctct cttgatgcct gccgccgcgt ggcatttgaa aacattgaag
    56641 atgcagcccg taacggcctg cactatgtcg agctgcgttt ttcaccaggc tacatggcaa
    56701 tggcacatca gctgcctgta gcgggtgttg tcgaagcggt gatcgatggc gtacgtgaag
    56761 gttgccgcac ctttggtgtg caggcgaagc ttatcggtat tatgagccgg accttcggcg
    56821 aagccgcctg tcagcaagag ctggaggcct ttttagccca ccgtgaccag attaccgcac
    56881 ttgatttagc cggtgatgaa cttggtttcc cgggaagtct gttcctttct catttcaacc
    56941 gcgcgcgtga tgcgggctgg catattaccg tccatgcagg cgaagctgcc ggaccggaaa
    57001 gcatctggca ggcgattcgt gaactggggg cggagcgtat tggacatggc gtaaaagcca
    57061 ttgaagatcg ggcgctgatg gattttctcg ccgagcaaca aattggtatt gaatcctgtc
    57121 tgacctccaa tattcagacc agcaccgtgg cggatctggc tgcacatccg ctgaaaacgt
    57181 tccttgagca tggcattcgt gccagcatta acactgacga tccaggcgtg cagggagtgg
    57241 atatcattca cgaatatacc gttgccgcgc cagctgctgg gttatcccgc gagcaaatcc
    57301 gccaggcaca gattaatggt ctggaaatgg ctttcctcag cgcagaggaa aaacgcgcac
    57361 tgcgagaaaa agtcgccgcg aagtaacaaa aatggatggt gctaattgca ccatcccctt
    57421 ttcacgcaag gcacaaagtc gcgcgatgtt tggcggattc gatgcccagc tcaatcaact
    57481 ccattacctg gattgcctga cttgccggaa ccggattttc accatcgcca tttaacgcat
    57541 cacgaatagc cgcatagtaa gccggataat tccccggcac tgtcaacagc gtttcttcga
    57601 cacgttcctc accttccacg cgggtaagta cgccatcgcg catatcgtag ccccagtctt
    57661 cctgcggcag acgctcgcca tttttcagac gttcttcctg tggatcgagg ccatatttca
    57721 cataactgcc tcgggatcca tgcacgatat accgtgctga ctcggctgct gccagcatgg
    57781 tgccgtgtaa aatcactcgc cgctgcggat aggacaagat agcgtggaaa taatcggtcg
    57841 actgcgctcc gggccgtaac tgtgccaaat ctaccgtcat gctaaccggt aaaccaaata
    57901 acgtaatggc ctgatcaaga aggtgcggtg ctaaatcgta ccagataccg ctgcctgggc
    57961 cgccctgttc acgccaacga tcgcgcacct gcgggcggaa gcggtcaaaa tgggactcaa
    58021 agtaagcaat ttcacccagc acgccttccg cgagcaaacc ttttagcgtc aggaaatcgc
    58081 tatcccagcg acggttatgg aatacagaca gcactcgccc caggcttttt gccagcgctt
    58141 ccagctctcg tgcttgtgac agtgtcacgg taaagggttt atcaacgacc acatgtttac
    58201 ccgcctcaag cgccgctttg gctaacggaa aatgggtatc gttgggtgta ggaatgacaa
    58261 tcaggtctat gttgggatcg ttaaacagat gcttcggctc agagacaacc gtaaccgttg
    58321 gccagtcagc ttttactttt gtttcatcgc tgctggagat taccgccagc tccagcccgg
    58381 gcgtgcccgc aatcaggggc gcatggaagg ttttgctcgc ataaccatac ccaatcaacc
    58441 caacacggat gttgtcgctc atgtcattgc ctctcattga agtatgatgg ctatttgaca
    58501 ctatccttta ccctcgctca acagtttaat aacctgccag caataaggga tgttgtttaa
    58561 cttaagtcaa aaaaatagcg atttttccaa cgacaaaagc taaatattgc aaaaacctca
    58621 gtaaaaatct tgctggagct attattgcta agtaacattt accccctgaa gttaatggat
    58681 caatcaacag agatgtgggc tgtaatgaat cgtcttattg aattaacagg ttggatcgtt
    58741 cttgtcgttt cagtcattct tcttggcgtg gcgagtcaca ttgacaacta tcagccacct
    58801 gaacagagta ctacagtaca acacaagtaa gctctgcact tgttgagcga catgctgccc
    58861 gtccgggtgc atgttttcac ttgtcggata ttaaaccagg aatttattat cttgttcgat
    58921 gttgttgatg attgtcaggg atagtaaagc gcgttactct ttccagagca gtttttcatc
    58981 cctgaatatt tgccatcagc acattcttat tattgtaata acacaagcgt tcacttttgg
    59041 tgaaatttta tcatcactaa taatacctac ccctttggta ttaattattt tatggacctt
    59101 ttatgactgt tcaggactac ttattaaaat ttcgcaaaat cagttcactc gaaagtctgg
    59161 aaaaactcta cgaccatctt aattacaccc tgacggacga tcaggaactg atcaatatgt
    59221 atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg cgggcgtttg tttgacctcg
    59281 gccaggtacc gaagtccgtc tggcgctatg tccagtaaag aaagtagcga tcggttataa
    59341 aagccttata ataacgcccc taaaattaga tttgtctgtt tgcgtcgcta ttctggagaa
    59401 cctatgacca ccacaacgcc acaacgtatt gggggctggt tgcttggccc gttggcctgg
    59461 cttttagtcg ctctgttgag tacgacgctg gcgttgttgc tgtataccgc tgcgttatct
    59521 tctccgcaaa catttcaaac gctcggcgga caagcactga cgacacaaat cttatggggc
    59581 gtttctttta ttaccgctat cgccatgtgg tattacacac tgtggttgac catcgctttc
    59641 tttaaacgtc ggcgttgtgt tcctaaacac tatattatct ggctgcttat ttccgtattg
    59701 ctggcagtaa aagcgttcgc tttttcaccc gtagaagatg gcatagccgt tcgccagttg
    59761 ctgtttactt tgttggcaac cgcattgatc gtcccctatt tcaaacgctc gtcgcgggtg
    59821 aaagcgacgt ttgtgaatcc gtaataacct tacagttaac ctgttgtcgc ctgctctgga
    59881 ttaacggata ataggcggct tttttatttc aggccgaaaa atgactgact acctgttact
    59941 gtttgtcgga actgtactgg tcaataactt tgtactggtc aagtttctcg gtctctgtcc
    60001 gtttatgggg gtttccaaaa agctggaaac cgcgatgggc atggggctgg caacaacgtt
    60061 tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat acgtggattt tgatcccact
    60121 taatctgatt tacctgcgca ccctggcgtt tattctggtg attgctgtgg ttgtgcagtt
    60181 caccgaaatg gtggtgcgca aaaccagccc ggtactttac cgtctgctgg ggattttttt
    60241 gccgcttatc accaccaact gtgccgtgct cggcgtggcg ttgctgaata tcaatctcgg
    60301 gcacaatttc ttgcagtcag cgctgtacgg tttttccgcc gctgtcggtt tctcgctggt
    60361 gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg gctgatgtcc cggcaccttt
    60421 tcgcggtaat gccattgcgt taattaccgc aggtcttatg tctctggcct ttatgggctt
    60481 tagtggtttg gtgaagttgt aatgaatgct atctggattg ccgttgccgc cgtgagcctg
    60541 ctgggcctgg cgtttggcgc cattctgggt tatgcctccc gccgttttgc ggtggaagac
    60601 gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga gccagtgtgg tcagtgcggt
    60661 tatcccggct gtcgccccta cgcggaagcc atcagctgta acggtgaaaa aatcaaccgt
    60721 tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg agttgcttaa tgtcgagccg
    60781 cagccgctgg atggcgaagc gcaagagcta acgccagcgc ggatggtggc ggttattgat
    60841 gaaaataact gtattggctg cactaaatgt attcaggcgt gtccggtaga cgccatcgtt
    60901 ggcgctaccc gcgccatgca tacggtaatg agtgatctct gtacgggctg caacttatgt
    60961 gttgatccgt gcccgacaca ctgcatctcg ttgcaaccgg tcgcagaaac tcctgactcc
    61021 tggaaatggg atctgaacac cattcccgtg cgtatcattc ccgtggaaca ccatgcttaa
    61081 gttattctct gcattcagaa aaaataaaat ctgggatttc aacggcggca tccatccacc
    61141 ggagatgaaa atccagtcca acggtacgcc cctgcgccag gtaccactgg cgcagcgttt
    61201 tgtcattcca ctgaaacaac atattggcgc tgaaggtgag ttgtgcgtta gcgtcggcga
    61261 taaagtattg cgcggccagc cgcttacccg tggtcgcggc aaaatgctgc cggttcacgc
    61321 gcccacctcg ggtaccgtta cggctattgc gccccactct acggctcatc cttcagcttt
    61381 agctgaatta agtgtgatta ttgatgccga tggcgaagac tgctggatcc cgcgcgacgg
    61441 ctgggccgat tatcgctctc gcagtcgcga agagttaatc gagcgcattc atcagtttgg
    61501 tgttgccggg ctgggtggtg cgggatttcc gacaggcgtt aaattgcagg gcggtggaga
    61561 taagattgaa acgttgatta tcaacgctgc tgagtgcgag ccgtacatta ccgccgatga
    61621 ccgtttgatg caggattgtg cggctcaggt cgtagagggt atccgcatcc ttgcgcatat
    61681 tctgcagcca cgcgaaattc ttatcggtat tgaagataac aaaccgcagg cgatttccat
    61741 gctgcgcgcg gttctggcag actctcacga tatttctctg cgggtgattc ccaccaaata
    61801 tccttctggc ggtgctaaac aattaaccta cattctgacc gggaagcagg ttccacatgg
    61861 cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc ggcaccgctt atgcagtgaa
    61921 acgtgccgtt attgatggcg agccgattac cgagcgtgtc gtgaccctga ctggcgaagc
    61981 aatcgctcgc ccgggcaacg tctgggcacg gctgggtacg ccagtgcgtc atttattgaa
    62041 tgacgccgga ttctgcccct ctgccgacca aatggtgatt atgggcggcc cgctaatggg
    62101 ctttaccttg ccgtggctgg atgtcccggt cgtaaagatt accaactgtc tgttggctcc
    62161 ctctgccaat gaacttggcg aaccacagga agaacaaagt tgcatccgtt gtagcgcctg
    62221 tgctgacgcc tgccctgcgg atcttttgcc gcaacagctg tactggttca gcaaaggtca
    62281 gcaacacgat aaagtcacca cgcataacat tgccgactgc attgaatgtg gagcttgcgc
    62341 gtgggtttgc ccgagcaata tccccctggt gcaatatttc cgtcaggaaa aagctgaaat
    62401 tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc aaagcgcgtt tcgaagcgcg
    62461 ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa cgacataaga gcgcagccgt
    62521 tcaacctgca gccaaagata aagatgcgat tgctgccgct ctggcgcggg tgaaagagaa
    62581 acaggcccag gctacacagc ctattgtgat taaagcgggc gaacgcccgg ataacagtgc
    62641 aattattgca gcacgggaag cccgtaaagc gcaagccaga gcgaaacagg cagaactgca
    62701 gcaaactaac gacgctgcaa ccgttgctga tccacgtaaa actgcagttg aagcggctat
    62761 tgcccgcgcc aaagcacgca agctggaaca gcaacaagcc aatgcggagc cagaacaaca
    62821 ggtcgatccg cgcaaagccg ccgtcgaagc cgctatcgcc cgcgccaaag cgcgcaagct
    62881 ggaacagcaa caggctaatg cggaaccaga acaacaggtc gatccgcgca aagccgccgt
    62941 cgaagccgc
//
LOCUS       DAZKHK010000011        57699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKHK010000011 DAZKHK010000000
VERSION     DAZKHK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.011"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(329..2275)
                     /gene="topB"
                     /locus_tag="ACHOS5_000718"
     CDS             complement(329..2275)
                     /gene="topB"
                     /locus_tag="ACHOS5_000718"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9775598.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKHFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQARSTPVRQFRGIVASGGGA
                     VKKAPNKRVKKAAKPKTDNTDSAN"
     gene            complement(2280..3323)
                     /gene="selD"
                     /locus_tag="ACHOS5_000719"
     CDS             complement(2280..3323)
                     /gene="selD"
                     /locus_tag="ACHOS5_000719"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP9775599.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3440..3991)
                     /gene="ydjA"
                     /locus_tag="ACHOS5_000720"
     CDS             complement(3440..3991)
                     /gene="ydjA"
                     /locus_tag="ACHOS5_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP9775600.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4152..6008
                     /gene="sppA"
                     /locus_tag="ACHOS5_000721"
     CDS             4152..6008
                     /gene="sppA"
                     /locus_tag="ACHOS5_000721"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9775601.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6175..7191
                     /gene="ansA"
                     /locus_tag="ACHOS5_000722"
     CDS             6175..7191
                     /gene="ansA"
                     /locus_tag="ACHOS5_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9775602.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7202..7843
                     /gene="pncA"
                     /locus_tag="ACHOS5_000723"
     CDS             7202..7843
                     /gene="pncA"
                     /locus_tag="ACHOS5_000723"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP9775603.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(7936..9294)
                     /gene="ydjE"
                     /locus_tag="ACHOS5_000724"
     CDS             complement(7936..9294)
                     /gene="ydjE"
                     /locus_tag="ACHOS5_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9775604.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKRIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(9412..10170)
                     /gene="ydjF"
                     /locus_tag="ACHOS5_000725"
     CDS             complement(9412..10170)
                     /gene="ydjF"
                     /locus_tag="ACHOS5_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9775605.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLTDFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(10307..11287)
                     /gene="ydjG"
                     /locus_tag="ACHOS5_000726"
     CDS             complement(10307..11287)
                     /gene="ydjG"
                     /locus_tag="ACHOS5_000726"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFP9775606.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(11297..12244)
                     /gene="ydjH"
                     /locus_tag="ACHOS5_000727"
     CDS             complement(11297..12244)
                     /gene="ydjH"
                     /locus_tag="ACHOS5_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9775607.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KTRQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            complement(12249..13085)
                     /gene="ydjI"
                     /locus_tag="ACHOS5_000728"
     CDS             complement(12249..13085)
                     /gene="ydjI"
                     /locus_tag="ACHOS5_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9775608.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFGSDGK
                     AE"
     gene            complement(13106..14149)
                     /gene="ydjJ"
                     /locus_tag="ACHOS5_000729"
     CDS             complement(13106..14149)
                     /gene="ydjJ"
                     /locus_tag="ACHOS5_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFP9775609.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKPMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(14166..15545)
                     /gene="ydjK"
                     /locus_tag="ACHOS5_000730"
     CDS             complement(14166..15545)
                     /gene="ydjK"
                     /locus_tag="ACHOS5_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9775610.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(15572..16648)
                     /gene="ydjL"
                     /locus_tag="ACHOS5_000731"
     CDS             complement(15572..16648)
                     /gene="ydjL"
                     /locus_tag="ACHOS5_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9775611.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(17018..17290)
                     /gene="yeaC"
                     /locus_tag="ACHOS5_000732"
     CDS             complement(17018..17290)
                     /gene="yeaC"
                     /locus_tag="ACHOS5_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP9775612.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(17332..17745)
                     /gene="msrB"
                     /locus_tag="ACHOS5_000733"
     CDS             complement(17332..17745)
                     /gene="msrB"
                     /locus_tag="ACHOS5_000733"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9775613.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            18087..19082
                     /gene="gapA"
                     /locus_tag="ACHOS5_000734"
     CDS             18087..19082
                     /gene="gapA"
                     /locus_tag="ACHOS5_000734"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9775614.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            19166..20050
                     /gene="yeaD"
                     /locus_tag="ACHOS5_000735"
     CDS             19166..20050
                     /gene="yeaD"
                     /locus_tag="ACHOS5_000735"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP9775615.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPLCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVVLTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(20101..20955)
                     /gene="yeaE"
                     /locus_tag="ACHOS5_000736"
     CDS             complement(20101..20955)
                     /gene="yeaE"
                     /locus_tag="ACHOS5_000736"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP9775616.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(21045..21791)
                     /gene="mipA"
                     /locus_tag="ACHOS5_000737"
     CDS             complement(21045..21791)
                     /gene="mipA"
                     /locus_tag="ACHOS5_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP9775617.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLHGYNPNDSWSPYLELSASYNFLGDWSVYGIAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            22227..24161
                     /gene="yeaG"
                     /locus_tag="ACHOS5_000738"
     CDS             22227..24161
                     /gene="yeaG"
                     /locus_tag="ACHOS5_000738"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP9775618.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            24274..25557
                     /gene="yeaH"
                     /locus_tag="ACHOS5_000739"
     CDS             24274..25557
                     /gene="yeaH"
                     /locus_tag="ACHOS5_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP9775619.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            25704..27179
                     /gene="cdgI"
                     /locus_tag="ACHOS5_000740"
     CDS             25704..27179
                     /gene="cdgI"
                     /locus_tag="ACHOS5_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9775620.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            27360..28850
                     /gene="dgcJ"
                     /locus_tag="ACHOS5_000741"
     CDS             27360..28850
                     /gene="dgcJ"
                     /locus_tag="ACHOS5_000741"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP9775621.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            28893..29396
                     /gene="yeaK"
                     /locus_tag="ACHOS5_000742"
     CDS             28893..29396
                     /gene="yeaK"
                     /locus_tag="ACHOS5_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP9775622.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(29397..29501)
                     /gene="yoaI"
                     /locus_tag="ACHOS5_000743"
     CDS             complement(29397..29501)
                     /gene="yoaI"
                     /locus_tag="ACHOS5_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP9775623.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            29671..30117
                     /gene="yeaL"
                     /locus_tag="ACHOS5_000744"
     CDS             29671..30117
                     /gene="yeaL"
                     /locus_tag="ACHOS5_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9775624.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(30074..30895)
                     /gene="nimR"
                     /locus_tag="ACHOS5_000745"
     CDS             complement(30074..30895)
                     /gene="nimR"
                     /locus_tag="ACHOS5_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP9775625.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            30992..32173
                     /gene="nimT"
                     /locus_tag="ACHOS5_000746"
     CDS             30992..32173
                     /gene="nimT"
                     /locus_tag="ACHOS5_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP9775626.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAIIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            32228..32575
                     /gene="yeaO"
                     /locus_tag="ACHOS5_000747"
     CDS             32228..32575
                     /gene="yeaO"
                     /locus_tag="ACHOS5_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9775627.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(32597..32851)
                     /gene="yoaF"
                     /locus_tag="ACHOS5_000748"
     CDS             complement(32597..32851)
                     /gene="yoaF"
                     /locus_tag="ACHOS5_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP9775628.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            33034..34059
                     /gene="dgcP"
                     /locus_tag="ACHOS5_000749"
     CDS             33034..34059
                     /gene="dgcP"
                     /locus_tag="ACHOS5_000749"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP9775629.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCVAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            34092..34190
                     /gene="yoaK"
                     /locus_tag="ACHOS5_000750"
     CDS             34092..34190
                     /gene="yoaK"
                     /locus_tag="ACHOS5_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP9775630.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            34193..34267
                     /gene="yoaJ"
                     /locus_tag="ACHOS5_000751"
     CDS             34193..34267
                     /gene="yoaJ"
                     /locus_tag="ACHOS5_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP9775631.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(34326..34574)
                     /gene="yeaQ"
                     /locus_tag="ACHOS5_000752"
     CDS             complement(34326..34574)
                     /gene="yeaQ"
                     /locus_tag="ACHOS5_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9775632.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(34722..34904)
                     /gene="yoaG"
                     /locus_tag="ACHOS5_000753"
     CDS             complement(34722..34904)
                     /gene="yoaG"
                     /locus_tag="ACHOS5_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP9775633.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(34908..35267)
                     /gene="yeaR"
                     /locus_tag="ACHOS5_000754"
     CDS             complement(34908..35267)
                     /gene="yeaR"
                     /locus_tag="ACHOS5_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP9775634.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(35440..36078)
                     /gene="leuE"
                     /locus_tag="ACHOS5_000755"
     CDS             complement(35440..36078)
                     /gene="leuE"
                     /locus_tag="ACHOS5_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP9775635.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(36205..36372)
                     /locus_tag="ACHOS5_000756"
                     /pseudo
     CDS             complement(36205..36372)
                     /locus_tag="ACHOS5_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            36373..36528
                     /locus_tag="ACHOS5_000757"
                     /pseudo
     CDS             36373..36528
                     /locus_tag="ACHOS5_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
     gene            complement(36621..37736)
                     /gene="rnd"
                     /locus_tag="ACHOS5_000758"
     CDS             complement(36621..37736)
                     /gene="rnd"
                     /locus_tag="ACHOS5_000758"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP9775636.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(37818..39503)
                     /gene="fadD"
                     /locus_tag="ACHOS5_000759"
     CDS             complement(37818..39503)
                     /gene="fadD"
                     /locus_tag="ACHOS5_000759"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP9775637.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(39708..40289)
                     /gene="yeaY"
                     /locus_tag="ACHOS5_000760"
     CDS             complement(39708..40289)
                     /gene="yeaY"
                     /locus_tag="ACHOS5_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP9775638.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(40329..41024)
                     /gene="tsaB"
                     /locus_tag="ACHOS5_000761"
     CDS             complement(40329..41024)
                     /gene="tsaB"
                     /locus_tag="ACHOS5_000761"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9775639.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLLDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(41082..42992)
                     /gene="yoaA"
                     /locus_tag="ACHOS5_000762"
     CDS             complement(41082..42992)
                     /gene="yoaA"
                     /locus_tag="ACHOS5_000762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9775640.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            43124..43468
                     /gene="yoaB"
                     /locus_tag="ACHOS5_000763"
     CDS             43124..43468
                     /gene="yoaB"
                     /locus_tag="ACHOS5_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9775641.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            43890..44189
                     /gene="yoaC"
                     /locus_tag="ACHOS5_000764"
     CDS             43890..44189
                     /gene="yoaC"
                     /locus_tag="ACHOS5_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP9775642.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(44309..44488)
                     /gene="yoaH"
                     /locus_tag="ACHOS5_000765"
     CDS             complement(44309..44488)
                     /gene="yoaH"
                     /locus_tag="ACHOS5_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP9775643.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            44562..45923
                     /gene="pabB"
                     /locus_tag="ACHOS5_000766"
     CDS             44562..45923
                     /gene="pabB"
                     /locus_tag="ACHOS5_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP9775644.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGNETVVCEGETSSTTTDDPLQVLQQVLDRADIHPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRKTVSLLSHNDV
                     NARRAWLENQQFSPQEDFALNSDWQSNMTRELYGEKFRKVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLQEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            45927..46505
                     /gene="nudL"
                     /locus_tag="ACHOS5_000767"
     CDS             45927..46505
                     /gene="nudL"
                     /locus_tag="ACHOS5_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP9775645.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            46689..48053
                     /gene="sdaA"
                     /locus_tag="ACHOS5_000768"
     CDS             46689..48053
                     /gene="sdaA"
                     /locus_tag="ACHOS5_000768"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9775646.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGFSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            48184..49782
                     /gene="pdeD"
                     /locus_tag="ACHOS5_000769"
     CDS             48184..49782
                     /gene="pdeD"
                     /locus_tag="ACHOS5_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9775647.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(49786..51342)
                     /gene="yoaE"
                     /locus_tag="ACHOS5_000770"
     CDS             complement(49786..51342)
                     /gene="yoaE"
                     /locus_tag="ACHOS5_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP9775648.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(51330..51488)
                     /gene="yoaL"
                     /locus_tag="ACHOS5_000771"
     CDS             complement(51330..51488)
                     /gene="yoaL"
                     /locus_tag="ACHOS5_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP9775649.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            51806..52777
                     /gene="manX"
                     /locus_tag="ACHOS5_000772"
     CDS             51806..52777
                     /gene="manX"
                     /locus_tag="ACHOS5_000772"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP9775650.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            52840..53640
                     /gene="manY"
                     /locus_tag="ACHOS5_000773"
     CDS             52840..53640
                     /gene="manY"
                     /locus_tag="ACHOS5_000773"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP9775651.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            53653..54504
                     /gene="manZ"
                     /locus_tag="ACHOS5_000774"
     CDS             53653..54504
                     /gene="manZ"
                     /locus_tag="ACHOS5_000774"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP9775652.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            54559..55017
                     /gene="yobD"
                     /locus_tag="ACHOS5_000775"
     CDS             54559..55017
                     /gene="yobD"
                     /locus_tag="ACHOS5_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP9775653.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWFEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            55446..56012
                     /gene="mntP"
                     /locus_tag="ACHOS5_000776"
     CDS             55446..56012
                     /gene="mntP"
                     /locus_tag="ACHOS5_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP9775654.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(56009..56818)
                     /gene="rlmA"
                     /locus_tag="ACHOS5_000777"
     CDS             complement(56009..56818)
                     /gene="rlmA"
                     /locus_tag="ACHOS5_000777"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP9775655.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDEKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELVRVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(56984..57193)
                     /gene="cspE"
                     /locus_tag="ACHOS5_000778"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(56984..57193)
                     /gene="cspE"
                     /locus_tag="ACHOS5_000778"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9775656.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(57206..57349)
                     /gene="yobF"
                     /locus_tag="ACHOS5_000779"
     CDS             complement(57206..57349)
                     /gene="yobF"
                     /locus_tag="ACHOS5_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP9775657.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT        14611 a        14594 c        13685 g        14809 t
ORIGIN      
        1 gagcagttat atatattcat tttgtgaata atgttcacaa tccctgcatt tgaaaataat
       61 ttataatgat gcaatccata acataaatta cgtgaatgta aagacatttc gtttataatt
      121 ttaactatgg gtacttatat catatactct aaataattcg agttgtagga agtcagaaag
      181 taagtgaatc ccgaggagct tacataagta agtgactggg ctgaacgaac gcagacgcag
      241 cacatgcaac ttaaagcatg acgagtataa ccaataaagc caatggcaga atatctacca
      301 ttggctttat ttaagagtgg aaaacgaatt aattagcaga atccgtatta tctgttttgg
      361 gctttgccgc ttttttcaca cgcttattgg gtgctttttt aacagccccc ccacccgatg
      421 ccacaattcc tctgaactgc ctgaccggag tgctacgagc ctgatcaatc agttggtaaa
      481 gcgtccccac cagcggctgc ataaagtcct gatagcgaca ctgcttttcg ctgatttgcg
      541 tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt cgccatttct ggcagcgaat
      601 ggaatagcgc ttttccggcg tcggtggagt ggatatagcg cccttttttg gtcaggaaac
      661 cacgcttgaa caacagttca ataatcccgg cacgggttgc ttctgtcccc aaaccatcgg
      721 tcgcacgaag gatctttttc agatctttgt cctgcacaaa gcgtgcgatc ccggtcatcg
      781 ccgaaagcag tgttgcatcg gtaaaatggc gtggcggctg ggtttgccgc tctaccactt
      841 cacctttttc gcacaacaat tcatcgcctt tcgccaccac aggcagcggc gtgccgtcgt
      901 tttcttcatc gcgctctttg ctgcctaaca gcgtgcgcca gcctgcttca gcaagaaaac
      961 gggctttagc gacaaatttg cctttggcaa tgtccagttc gataacacac ttgcggaaca
     1021 ccgcatccgg gcagaactgc atcaaatact gacgggcaat caggttatag accttcgctt
     1081 cgttctccgt caggttgatc gcagaactcc gtgcggtcgg aatgatggcg tggtgcgcat
     1141 cgaccttttt gtcatcccaa cagcggttgc gtatatctgg atctaccact ggctgcggca
     1201 acagatccgg tgcatgaacg ctgatagcat tcatcaccgc gtggcgtccg gcaaaatgtt
     1261 cttctggcaa atagcggcaa tcggaacgcg gataagtgat tagcttgtgc gtttcgtaca
     1321 gcttctggca gatatcaagc acgttctgcg cgctcagacc aaaacgttta gccgcttcaa
     1381 tctgcaacgc cgaaagcgaa aaaggcagcg gcgcggattc tgattcccgt ttatcgttat
     1441 agctggtgac aatagctggt tgaccactaa tgcggttaac cacatgctcc gccagtggac
     1501 gatgtaacaa gcgcccttct tcatcctggt acggttcaca cgcttcgctc ggttgccaga
     1561 tagcggtaaa ccgctcatct gcaggtgtca cgatatgtgc tttgacttca aagaagtctt
     1621 tcgccacgaa gttttcaatc tcttcgtcgc ggcgcaccac cagtccgagt acgggcgtct
     1681 gcacgcgtcc cacggaaagc acgccctgat aaccggcatt gcggccgaga atggtatacg
     1741 cacgggtcat attaatgccg tacagccagt cagcccgcgc ccgcgccagt gcagaaacgc
     1801 acagcggtac aaactcgcta ttggagcgaa gacgatcgat cgcccgctca accgcctgcg
     1861 ggttcaggtc gtttatcaaa caacgctgta cctgctggcg cttttccggt gctagttgca
     1921 ggtagtccag cacttcatcc accagcagtt gcccttcgcg atccgggtct ccggcgtgaa
     1981 cgatttcgct ggcttcatgc aggaagtgtt tgatgacatt aagttgttta gtcacggagg
     2041 gtcggggctg taattgccac ttttccggga caattggcaa atccgcaaga ttccagcgcg
     2101 catagcggct gtcgtaggcg tctggctgcg cctgctcaag caggtgaccg atacaccagg
     2161 tcaccacctg accattaccg cactcgataa agccatcgcc tttccggtgc ggtttaggca
     2221 ggacatcagc aatggcgcgc gccagactcg gtttttcggc aataaacaac cgcattgaat
     2281 taacggatct caaccatggc acgaccgccg cgcgctggca ccagttcgcc aatcgccgtc
     2341 agttcaatgc caaactcggc ggctgtagct ttgacctcat tttctgcttc cggcatgacc
     2401 gccagcagca aaccgccaga agtttgcgga tcgcacagca gatcgcgcac ttcacgcggc
     2461 atttcaccca tcagatgacc gtagctggca aagttacgtt cggtgccgcc aggcactgcg
     2521 cccaacttaa tgtactcttc aacccccggc aatttcggga tcgcgtcata gtcgacgcgc
     2581 gcctgcacac ctgcgccctg acacatttcg cttaaatgcc ccagtaaacc aaaacccgta
     2641 acgtcggtca tcgcttttac gccttcgatg ttggcaaagg acgcgcctgc gatgttcatc
     2701 cggcacatca cttccgtcgc cagtccctga tgttctggtt tcagcagtga ttttttctca
     2761 gccgtggtaa gaacgccaat ccccagcggt ttcgtcagga acagtttgca tccggcttgt
     2821 gcggtactgt ttttcttcac ccgctcggtc ggtacgatcc ccgttaccgc cagaccaaaa
     2881 atcggctccg gcgcatcgat ggagtgaccg ccagccagcg caatccccgc ctgacggcat
     2941 gcatagcgtc caccttcggt cacttcgcgg gcaatttccg gggaaagttt gttaatcggc
     3001 cagccgagga tcgcaatcgc cataatcggt ttgccgccca tagcaaagat atcgctgatg
     3061 gcgttagtcg ccgcaatgcg gccaaaatcg aaaggattat caacgatcgg cataaagaag
     3121 tcggtggtac tgataacgct ggtgccattg cccagatcgt ataccgccgc atcgtcgcgg
     3181 gtttcattcc ccacaagcaa attcggatca acaaacttcg cctgctcact gtgcaggatg
     3241 gtttccaaca cttttgggga aattttacag ccgcaaccag cgccgtggct gtattgggtc
     3301 aaacgaatcg agttctcgct catggacatc tcctgtcaat gcaatccggg tatggtaacc
     3361 ctcattccat gaagtgataa gtgagagtgt ctgaattcct gcgcctttgc tcacaatcca
     3421 gacagtttcg cgacaattat cagaaataag tcacaaacgg cgtcgggtcc gggacgttta
     3481 tcgacgtaga tgctttcaac tgcggcgtac cgaggtagag aaaaccgaca attttatcct
     3541 gctcacggca accgaatgct tcacggacta ccggactttc agttaatgca ccactgcgcc
     3601 agatgccgcc aaacccctgg gcaactgctg ccatttgcat cgccatgacc gcgcatcccg
     3661 cagacatttc ctgttcccag cgcgggactt tatgattttc ttcgcatttc gccaccaccg
     3721 ttatgatgag cggtgcgcgg aacggcgcat tacgcgcttt gtcgattgct ttgttatcac
     3781 taccggcagc aatcgccccc tgctccagta cagcgctgaa acgctcgcgc ccttcccctt
     3841 caatcacaaa gaaatgccac ggttgcatgg acttatggtc cggcgcacgc atacccgcac
     3901 gcaggatgtt ttgcagttgt tcacccgttg gcgcgggttc agccaggcgg gaggcgctac
     3961 ggcgattgat caatagttcg agtgcatcca tttgattaac tcctgtcatg atatttattc
     4021 acaaaattaa cacgagagtg gattttgtta cagcccagtc cgcaattcct gctgacaagt
     4081 gccggttggg tcattacgat aaccacatct attgcgcctg aaacaggtgt gaccttaagt
     4141 tgggagaata catgcgaacc ctttggcgat ttattgccgg attttttaaa tggacgtggc
     4201 gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt ctttattttt ctcgtgctgg
     4261 ttggtgtggg gatctggatg caggtcagtg gtggtgattc gaaagaaacg gccagtcgtg
     4321 gcgcactgct gctggacatt tctggtgtga tcgtcgataa acccgacagt tctcagcggt
     4381 ttagtaaatt aagccgccag ctgcttggtg ccagttccga tcgtctgcag gaaaactcac
     4441 tgtttgatat cgtcaacacc attcgccagg cgaaggacga ccgcaatatc accggtattg
     4501 tgatggatct gaaaaacttc gcaggcggcg accagccgtc tatgcagtac atcggcaaag
     4561 ctctgaaaga gtttcgtgat agcgggaaac cggtttatgc cgttggcgag aactacagcc
     4621 aggggcaata ttatctcgcc agtttcgcca ataaaatttg gctgtctccg caaggcgtgg
     4681 ttgatctgca cggctttgcc accaacggtc tgtactacaa atcgttactg gataagctga
     4741 aagtttccac ccatgtgttc cgcgtgggta cgtataaatc tgccgttgaa ccgtttattc
     4801 gtgatgatat gtcgccggca gcccgcgaag ctgacagccg ctggattggt gagctgtggc
     4861 aaaactatct gaatactgtt gccgctaacc gccagatccc tgctcagcag gtattccctg
     4921 gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg cgataccgcg aaatatgcgc
     4981 tggaaaacaa gctggtcgat gcactggcat cgagtgccga aatcgaaaaa actctgacca
     5041 aagagttcgg ctggagtaaa actgataaaa attatcgcgc catcagttat tacgattacg
     5101 cattgaaaac gccggcagat accggtgaca gtatcggtgt cgtcttcgct aatggcgcaa
     5161 ttatggatgg cgaggaaact caggggaatg ttggcggtga taccactgcg gcacaaatcc
     5221 gcgacgctcg ccttgacccg aaagtgaaag cgattgttct gcgtgttaat agtcccggcg
     5281 gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc agcagcccgg gcagcgggta
     5341 agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg tggttactgg atttccacac
     5401 cagctaatta cattgtggct aaccccagca ccctgacagg ttctatcggt atcttcggcg
     5461 tgatcaccac cgtagaaaat agtctggatt cgattggtgt tcatactgat ggtgtctcaa
     5521 cttcaccgct ggcagatgtt tctatcacca gggcactgcc gccggaagcg cagcagatga
     5581 tgcagttaag cattgagaat ggctataaac gctttatcac gctggttgct gatgcgcgtc
     5641 attcaacgcc ggagcagatt gataaaatcg cccagggcca cgtctggacc ggccaggatg
     5701 caaaagctaa cgggctggtc gatagtctcg gggatttcga tgatgcggtc gccaaagcag
     5761 cagagctggc aaaagtgaaa cagtggcatc tggaatacta cgttgatgaa ccgaccttct
     5821 tcgacaaagt gatggacaac atgtctggtt ctgtccgggc aatgttgcca gatgcgttcc
     5881 aggccatgtt acctgcaccg ctggcctcgg tagcctctac cgttaaaagt gaaagcgaca
     5941 agctggccgc gtttaacgac ccacaaaacc gttatgcgtt ttgcctgacc tgcgccaacg
     6001 tgcgttaact cttgtactaa gtggccgaca gatcgtcggc cacattactt tttacgtcga
     6061 cgaatcctct tcccgctgtc tcgccacata tttccttatc ctcatagtat caggtgcgct
     6121 ccccctgcct cacgtatata cttttgctct ttcgatatca ttcatatcaa tatcatgcaa
     6181 aagaaatcaa tttacgtcgc ctacacgggc gggaccatcg ggatgcagcg ttccgagcag
     6241 ggttatattc cggtgtcagg tcatctacag cgccaactgg cgctgatgcc ggaattccat
     6301 cgcccggaga tgccagattt caccattcat gaatatacgc cattgatgga ttcttctgat
     6361 atgacgccag aagactggca gcatattgct gaagatatta aagcgcacta tgacgattat
     6421 gatggttttg tcattctgca cggtacagac acgatggcgt ataccgcctc tgcactgtcg
     6481 ttcatgctcg agaatctcgg taaaccggtc attgtgacag ggtcacaaat cccgctggct
     6541 gagttacgct ctgacggaca aattaatctg ctgaatgcgt tgtacgttgc ggcgaattat
     6601 cctatcaacg aagtaactct cttttttaat aatcgactgt atcgtggcaa cagaaccacc
     6661 aaagcccatg ccgatggttt tgatgcgttt gcctctccaa accttcctcc gttactggaa
     6721 gcaggtatcc atattcgccg tttgaatacg ccacccgccc cgcacggtga aggggaattg
     6781 atcgttcatc caatcacgcc gcaaccaatt ggcgtagtaa cgatttatcc ggggatttct
     6841 gctgacgtcg tgcgcaattt tctgcgccaa ccggtgaaag cattgattct gcgctcatat
     6901 ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg aattacaaga agccagcgat
     6961 cgcggtattg tggtggtcaa cctgacacaa tgtatgtctg gtaaagtgaa catgggtggt
     7021 tatgccaccg gtaacgccct cgcccatgcc ggcgttattg gcggtgcaga tatgactgta
     7081 gaagccacac taaccaaact gcattacctg cttagccagg aactggatac tgaaaccatt
     7141 cgcaaggcca tgagccaaaa cctgcgtggc gaactgacgc cggatgatta aggagactgc
     7201 aatgccccct cgcgccctgt tactggtcga tttacaaaat gatttctgtg ctggtggcgc
     7261 gctcgccgtg ccggaaggtg acagtacggt ggatgtcgct aaccgcctga ttgactggtg
     7321 ccagtcgcgc ggtgaagcgg ttatcgccag tcaggactgg cacccggcga atcacggcag
     7381 ttttgccagt cagcacggtg tagagcctta tacgccgggc caactcgacg gtttgccaca
     7441 aaccttctgg cccgatcact gtgtgcagaa cagtgaaggc gcacaattac atccgttact
     7501 gaagcaaaaa gcgatcgcag cggtgttcca taaaggcgaa aatcctttag ttgacagtta
     7561 cagtgccttt tttgataacg gccgtcggca gaaaactgcg ctcgatgact ggttgcgcgc
     7621 tcatgtaatc aatgaattga tcgttatggg tctggctact gactattgcg ttaagtttac
     7681 cgtgctggac gcgttacagt taggttataa ggtgaacgtg attaccgatg gttgtcgtgg
     7741 cgtgaatatc cagccacagg acagtgcgca cgcgtttatg gagatgtcag cagctggggc
     7801 aacgctatat acgctggcag actgggaaga aacgcaggag taattttacg ctggcctaca
     7861 attctgtact ggcattgtag gccagataaa acacatcagt gatacatctg gtaattgacg
     7921 ccatcaacga aagatttaat tcacctcaga aatctcttcc agcgacacct tccgcgtttc
     7981 gatgccaaaa atggagagaa ccagcgcaca gagcactaac atgacaccaa gcaccataaa
     8041 caccgtgatc gacccataat gtgttaataa tgccgcaacg ccatagggcg tgaagactgc
     8101 gacgatccgt ccgacggcat taacgaaacc cgaaccacgc aggcgtaaat gcgttggcca
     8161 aagttccggg atataaaccg ccgacgcgaa gcacacatac atgtataaaa agaagatcat
     8221 caccaggcca tagattaaaa tcgcccactc tgtagtctga attgaataga tatagcctaa
     8281 cacagcaata ataataagta aggcagagcc aaataatcga cgaggaaaat gatcaataat
     8341 taatgccgca ataaatatcc ctaccggagc gccaatcata ataacagcgg tcattaatat
     8401 tgatttatca acatcaatgc cggagttaac aaatatggtc ggtatccata cggtgatggt
     8461 ataaagcgaa atgttcattg caattaaaac agtaatcgcg actaatgtac gtcgtaacat
     8521 ttccccttta aacaggagcc agaaggtacc cttgattacc ttaaccttgc tgttgctctg
     8581 gttcaaagta agcgggggta aacgaatcct cttctctctt tcaatttgct gctctacttc
     8641 acgaagttgg ctttctgcac ctgcgatttg ccctttccct gccagccatc gtggcgactc
     8701 aataaagtat ttacctgaga gaaaccacgc taacagtatg ccaataccgc ccagcagaaa
     8761 cattatccgc caactaaaaa aagcgataac caccacgcct atcgccgcag agagcatggg
     8821 cgaccagtta ccaacaaatg agagccgcgc ggaccatttt ccacgcaccg tcgcggggat
     8881 aaactccgta aatgaggcgt agccaaccat aatcagcgcc cccattcctg ttcccatcag
     8941 gaagcgaaag aagatgagcc agtacatatc agggacaaaa gcggcccctg ttgccgcaat
     9001 accgacgatg agaagattta tgcgaaacgc cctgcgccgc ccaaagtagt caccaataaa
     9061 cccaccggta agagagccga tgaaataacc aaacattaat gccgaggtaa aggcggcatt
     9121 gaggaaatta tttgaccatc cattgcttac cagctttgct aagacgacat taccggagta
     9181 actcaaaaac cccgttaata acagactaaa gcttattata ccaaatatac gataatgaaa
     9241 ccgggccaaa ggcaagcggt ccagtcttgc gccaatttga tcatattgtt ccattgggtg
     9301 cctcggattt gctaaaaaag gtagcaattg cggcaaattc acttgccgca tctttatcat
     9361 cattttattt ccaggcgcag gaacaaataa tattgacaat gtgtgcttaa attaccacac
     9421 cagctggata ttattgtctt tgcaaaatgc tatccactct gcacccggtg atttatcagt
     9481 tattatgtaa ttaatatgac taaagtcagt taactggaca aaagctttgc gatcaaactt
     9541 agagtgatca accaataacg caacttctgt cgcctgacgg atcattgtct ttttgatttc
     9601 agcttccgct tcattagagt ccagcgcgcc gctgttaata tcaagacctt tacaactcat
     9661 taccatgata tcgacatggt agcggctgat gatctcttta gtaattcttc cttgcagtga
     9721 aagtgtattt ttgtttagtt ccccacccgt tgaaacgact ttaatttctg actgagccag
     9781 aacatgaatc gcttccgccg agttggttag caacgtcagg tcactacggt cctgtaacaa
     9841 tttgagcaat tccattacgg tactactgga atcggctgcc atggtggttt tattgtcgat
     9901 aaagggtagt gccttgcgtg caataagctg cttctcttca taaaacgatg aagcgcgctt
     9961 ataaaaatgg atattctccg tcaacatcgc tgtatttaaa acagcaccac cataggttct
    10021 ggtcaaaaag ccttcatctt ccaacttctc aagatcgcgg cgaatggttt cttcggttac
    10081 ctgaaaaatc ccactcaaat ttgagactgt caccttttta tcgttggcaa ccatttgctt
    10141 aattgcctga atcctgtctt ttgccgccac gattacaccc ctgtatcttt ttacatcaca
    10201 ttagcgcaat tatcgcataa ccgatgttta ctttcaaaat aacctgtttg aatcacagat
    10261 tttcatcaca gttttcacag aaacagaggt gaatcgtgtt gagtatttaa cgctccaggg
    10321 cctctgccat ttccctcatc aatgttgcgt ctgcatccga taagttgata ttcagtgccg
    10381 cgacattttc acgtacctgt tccggcgcag tagccccact aagaatggag attaaatcac
    10441 tctgttttaa tatccacgcc agtgccagag tgggaattgt gcactgataa cgagcacaga
    10501 gtggctgcca ctgttcaagc atatcaatca ctttcagcat gttttcacgc tggaaccaga
    10561 ctttatttgc ccgagcgccg cccggaacgt aatcacgcgt gatggtcccg gtcaacaatc
    10621 cctgctctag cggagagtag acctgtacca caatgccatt atcacgacat agcggcagca
    10681 gttcgttttc cattgcccgg tcgaggatac tgtatttcgc ctgaataata tccagttcac
    10741 catgttgcag atactcgcgg atatggtcag catcgacgtt agcagcgcct atagcgcgaa
    10801 ttttcccttc ggcctttaac gcattaagca ctgcgacagt ttcagctatc ggcgtaaaaa
    10861 atggcggcac cgactgccag tgcgtcatgt agatatcgat gtaatcaata cccagacgtt
    10921 gcaagctggc ttctacctct tcgcggatag attccggaga aaggttttta tacaactgtc
    10981 gatcgccaac tttgttgaat aaactccctt ttcgttccca gacaatgccg catttggttt
    11041 ctactacaac ctgttcacgg ggcagttttt ttaacgcctg accaacgata acttcactat
    11101 tgccaaagtt atatcctggc gcagtatcaa tcaggttaat gccgcaacga tgggcttcaa
    11161 gaatcgtatc aatgcatatt tgtcgatcga gatcgccatt ccatgcagga ccgccgccaa
    11221 tggcccatgt ccccaacccc attcgcgaaa gcgttatatc cgttgtgcct aaaggtatct
    11281 ttttcatttg ccttcattat ccttcgtatt cttccagcaa ttgttcgacc agctttctgt
    11341 tttttacacc ggtggtggcg ccgatgctta gaaccgagat agccgccgtt gcattggcaa
    11401 agcgtgcgca ttcacgcaga tttttacctt ctaacagtgc tgcaataaaa cctgaagcaa
    11461 agttatcgcc cgcaccaatg gtgtcgatag cggttattcc tgcgaccgcc ggcaccttca
    11521 tcgtcatgtc accacgcttg ataaagcagc cgtctttacc cgttttaatc accaccgttt
    11581 ttacgccgca cgcaagaaag cagtcagcaa tttcatccag tgtctctttc ccggtgagta
    11641 attttgcctc ggcaaaatta ggaaacagat aatcgacata actcaatgct tcgcaaatat
    11701 cattcagcgt ttcattcaac cgcggtttga tcatatcggc acagataatc atctgccggg
    11761 ttttagcttg cgtaaaaatt tctgttagcg ctctaccatc caatagagga ctgttgaaaa
    11821 tactggccag tgataataat ttcgcctgag aaaaccgcgc aaaatcaaca tcgtcaatat
    11881 tcaatttcca cagactgccg ttacggtttg tgacaaaggt ccgctcgcca tcctccgtca
    11941 ccagtccaac gttaatagag gtatctatgc tgacgtcctg cttcaggctt tgaatatcaa
    12001 tattctcttt gcggcaatgg tcgagaataa attgccctgc ggcatcttta ccaatacgac
    12061 tcattaatgc tgtgcgatgg cccagacgag aaataattgt tgcttcattt attgcatccc
    12121 ccccggtggt cattgcgatt ctttcaagag gataagaatc cacatcaaag atatttttac
    12181 tgaccggttg caatggaata tcaacaatag cggcacctat acaaataacg tcgagattat
    12241 ccatattatt attccgcttt gccatcagaa ccgaacagtt taattttatc caatgcccgt
    12301 tcttttacag ctttacgcac ttcacgttct aaatgcagga aaggctgatc ctggttttct
    12361 ttcactgcta ccattgcggc ctgacagagc tccgtatgaa tgttgatttt cgcaataccc
    12421 aacgaaattg cagttttaat gtcggcatca ctgatccctg atgcgccatg caaaaccagc
    12481 ggaacagaaa cagcatcgcg tacgcgtttg acgacctcaa agttcaattg tggctctgac
    12541 gtataaaccc catgctggtt gccgatggcg acagccaatg agtcacagcc cgtacgttca
    12601 acaaattcag ccgcctggtc aggatcggta tagtgatagc ccgccagcgc ttcctcataa
    12661 acggtttcat taccgacatg ccctaattcc gcctctaccg gaatacccag cggatggaag
    12721 aaatcaacag cctctttggt taaacgaata ttttcttcga aatcaaatgc agaagcatca
    12781 cgcattaatg aattcatacc atgagtccag gcgttatgaa taatctccat actccgacca
    12841 tgatcccaat gagttattac cggcaccgtt gctttttgtg ccattgatac catcatgtga
    12901 gagaaatctt caaatgaggt gttgccgaca aaacctgtgc caaaagaaat aataaccggt
    12961 gatttcgctt cttcggctgc gtcgataacg cccatcaaca tttctgcatt ccatacgtta
    13021 aaatgggcaa ttgcataatg tttatttgtg gcatcgtttt cccaatatct gatatctgcg
    13081 agcatgttct gttctcccgt attaattaat cgctaatttt aataacgcct ttaataatgt
    13141 cgcgtttgtt gttaactgac tcttcaaatg cctgttgtac atcccgataa tcgtaaatat
    13201 gcgtcaccat cggtttcaca tcgaatcgcc ctgaggaaat agcttcaatc gtgaccggat
    13261 aacgattggc atagcggaat accgtctgga tagtgacttc gcgattgatt ttgaggaaat
    13321 tgattgccga atcgccgggt acagtaccaa caatcataat tttaccgccg cgcatgacca
    13381 gataaggtgc ctgtttaacg gtgaccgcag aacccgctgt ttcaaaaaca atatctgcgc
    13441 ccatgtcttc ggtaaattgc tgacagcgtg caatagtgtc ttcttttgcg ccgttaataa
    13501 ccactgtcgc gccgagctgt tccgccattg tcagacgttt ttccagcaca tcaacgacgg
    13561 caatttccgt tgctcccagg catttgcacg cttgcaacgt catcaaacca atacaacccg
    13621 ctcccagaat aattatcttc ttaccaggtt taacatctgc cagcatcgcg gcatgcatcc
    13681 cgactgcggc aggctccacc agcgcccctt ccatcgtgtc catattgtct ggcagtttgt
    13741 aagtaaagct ctccggatga cacagatagt gcgttaatgc gccgcggtag ttgggttgtg
    13801 tcgccataaa atcaacgtcc gggcagatgt tatatttgcc ttccagacag taacgacagt
    13861 gaccgcaagg aacgccaggt tcgatattta cccgatcccc cggtttaaat ttgcgcacgc
    13921 ggcttcccac agccacaacc gtcccggcac attcatgacc caggccaatt tcttgatttg
    13981 ggtctttagg aggaataaac gggcctgatt caaaaccatg tacatctgaa ccacaaatac
    14041 cgacatattc cactttaatc aaaacttcat cttctttagg cacaggtatt tctgctgaaa
    14101 taattttcat tgtgcccggc acctgcaata ttgcttttga atttttcatt ttgaatccac
    14161 cagtattatt tattggctac tgcatcaata cttaaacttt caacggagac acctttggtt
    14221 tcaattccaa tggtggcgat agcaattgcg acaataattg aaacagcccc cagaagaata
    14281 aagactcccg ttacgccata actactgagc agcactgcaa cggcataagg cgcggcaata
    14341 ccactgattc gccctaccgc attcgccaga ccggagccac ggagtttggc ctctgtcggc
    14401 cagatctcag ggacatacac tgccgaggca tagcaaacgt acatatagac gaaagtaatc
    14461 aggaagaaac caatcagcgt tatgagcaac atactggttt gcagtgaata gatatatccg
    14521 agcaccgcaa tcaggattaa tagccccaca cccattgttt tacgcggaat tttatccatc
    14581 accagcatgg caataaaaat accaaatggc gcgccaaaca tactcatggt atttaaaaca
    14641 atcgagtctt ttaaattaat tccctgggtc atgaatatcg ttggcaacca gttaattagt
    14701 gtgtactgca caacgttcat ggcaatcagc acacaagagc ctaatatcac gcgtttcagt
    14761 aatactcctg tcagtaaggc tgaatacggc accgcttgtg gtgctttacc gtcatcagca
    14821 ataaccacag gcggcaaagg tttacccgtc tggcgtatga cgccttcttc tatactccgc
    14881 atgactttct cggcttcctg ataacgtccg cgcgattcca gccagcgcgg ggattcagga
    14941 aagtagcgcc aggccagcgc cgtagcgata agtgacaata ttgcagggat aagcagttgt
    15001 acccgccagt tccactctgc actaatcagc ggcgtgagtc ccatcgcaat caatgaacag
    15061 agcggatatg accagttacc aataaaggaa acccgactgg accacgttcc gcgatttctt
    15121 ccgggcatat attcggtgaa accagcaaac agtgtaacca gtaaagctcc cagcccaacg
    15181 cccatcacaa aacggcaggc aataaggaaa tccatattcg gtgagaaagc accgacaacc
    15241 atcgaagcaa tatgaatggc ctcatagagg atgaacgcat ttctgcgacc ggttttgtca
    15301 ccgatgatgc cgcccaccag cgcaccgaga aacattccgg cggtcgtgat tgctgagaat
    15361 gtggcagtgg tggaattatc tgtccagccc aacgctttca gctgcgcgag gatcaagcca
    15421 ccaacggcat tactccagca gacaagcaag ccaaacgcga cgatggcaaa cattgacgaa
    15481 tgccagcggc aatccggtaa gcgatccagc cgtgcaccac aatgcggttt tgttatctgt
    15541 tccatgcgat aacgttcctt caggagagta gttattcatc aaagtcgtaa gtcatgatca
    15601 ctttgattgc agttttatcg accatcgcat caaacccttc gcgccattgc gacaggccga
    15661 tacgatgcgt gatcatcggt ttgactttga tcgcgccgct ggctaatagc ctgatagcgt
    15721 tacgccatga ggtggagtca taggccatat gcccaatgat gcttttgttc caggcggtaa
    15781 tgtcattaat cgagaaatca agaggtttga agcccattcc aacgcgtacc acttccccgt
    15841 tcggacggag catttcgatg gcttgtttca gtgcgatatt ggcaccggag cattcaatca
    15901 ccagtcccag attgtctttg ccacaaattt gctggcagcg cgccaccaca tcttcggtag
    15961 aaccatttac tactgccgtc gcacccagtt cttttgcaac cgggaagcgg accgccacat
    16021 cttcttgtag accaacgacg acgatattta ccgcccccat aatccgcgcc atttgtacgg
    16081 agaacagccc gagtgggcca gtgccgatga cgaccacatc ctgaccaggg aggaatttcg
    16141 attgctgcgc gatggatttg taggcattac aaatagggtc aagtacggct gcgtcctcat
    16201 aatcaacacc atcagggatt tcccacaacg catgacgatg aattttgaga atttcaccag
    16261 gaacaagaca atatttggaa aaaccaccgc cccaggtatt gttatccaga ccaaggttta
    16321 ctttttctgt acaacacaga aaatcacctt gttcacaggc cgggcaaaca ccgcaaacgt
    16381 gaccgctgtt atccgacacg acgcgttgcc ccactttcca gtctttgact ttttcaccaa
    16441 cctgcgcaat acaacctgcg aactcatggc cgcggataga gttaaactca tcagaaccgc
    16501 tatcgacatt gtagtgcttc atgtctgcgc cgcagattgc cgcggcttta atttcaatca
    16561 cgacatcttc cgggccacaa atgggttgtg ggacatcaat catcttgtag ccgccaaagg
    16621 ccttgccaaa ccgagccagt gctttcattg cgtcatcctc tgtgttttgt tttgcttaga
    16681 tggtcatcat acttacccac atgatttttg aggtcaacat caaatctgta aaacacagat
    16741 aatgatctgc gttttacaac ttggatcaca attacgcaga aaatggctgg ggctgttaca
    16801 ggttgatgga aggcgggacg caaaaagagc aaattcgaga gggattcccg gccaaatagg
    16861 cctgagactt aagttcagga gaagtcattc aggtgtgaac gcgactgcaa gagatcggac
    16921 tgggacttag ccaggtcggg gcgagataat tgagatgagt cgtggggtca ttgttggcta
    16981 tcccctttat aaccgcggca aaaaggggat aaaccactta cttaaacatg gcaataggct
    17041 ttgctgagat accaaaatct tcttttaact gctgtttgct cttcatgacc atctgaccat
    17101 ttgtgtcaat tgtcatatgc tgtgcttcgg tattatggcg ggcttgccac agcatcacca
    17161 gttgcaggca gttctctttt tgttcctcgg tcaacgtaac gccatcaggc catttcccca
    17221 gttcaacggc ggtcgacaaa cgctggtata cttcaggcgt catgctgttg ataatgtcat
    17281 caagattcat aatttttccg ctcctgtgga ataagttgct gaatcgtttt ttcaaccatt
    17341 aatttcttcg ccgttttcgc catcagtgaa gcgtaaagaa gcagagttaa cacaataacg
    17401 ttcgcccgtt ggctgcggcc cgtcagggaa gacatggccc agatgggcat cgcagttacc
    17461 gcaacgaatt tctatgcgct gcattccatg tgacaagtct ttgatataac gaatagattc
    17521 ttcactcacc ggttcgtaga aactgggcca gccacagccg gaatcatact tggtttggga
    17581 atgaaacagc ggggcatcgc agatcaaaca gtgatatacg ccgtcacgct tgttatgcag
    17641 taaacgaccc gtaaatggcg gttctgtccc atgattctgc gtcacgtaga actgcatctc
    17701 ggacaaattt tttttcagtt cttctgccga aggtttatta gccatttgct cacatctcac
    17761 tttaatcgtg ctcacattac gtgactgatt ctaacaaaac attaacacca actgacaaaa
    17821 ttttgtccta aacttgatct cgacgaaatg gctgcaccta aatcgtgatg aaaatcacat
    17881 ttttatcgta attgcccttt aaaattcggg gcgccgaccc catgtggtct caagcccaaa
    17941 ggaagagtga ggcgagtcag tcgcgtaatg cttaggcaca ggattgattt gtcgcaatga
    18001 ttgacacgat tccgcttgac gctgcgtaag gtttttgtaa ttttacaggc aaccttttat
    18061 tcactaacaa atagctggtg gaatatatga ctatcaaagt aggtatcaac ggttttggcc
    18121 gtatcggtcg cattgttttc cgtgctgctc agaaacgttc tgacatcgag atcgttgcaa
    18181 tcaacgacct gttagacgct gattacatgg catacatgct gaaatatgac tccactcacg
    18241 gccgtttcga cggtaccgtt gaagtgaaag acggtcatct gatcgttaac ggtaaaaaaa
    18301 tccgtgttac cgctgaacgt gatccggcta acctgaaatg ggacgaagtt ggtgttgacg
    18361 ttgtcgctga agcaactggt ctgttcctga ctgacgaaac tgctcgtaaa cacatcaccg
    18421 ctggtgcgaa gaaagtggtt atgactggtc cgtctaaaga caacactccg atgttcgtta
    18481 aaggcgctaa cttcgacaaa tatgctggcc aggacatcgt ttccaacgca tcctgcacca
    18541 ccaactgcct ggctccgctg gctaaagtta tcaacgataa cttcggcatc atcgaaggtc
    18601 tgatgaccac cgttcacgct actaccgcta ctcagaaaac cgttgatggc ccgtctcaca
    18661 aagactggcg cggcggccgc ggcgcttccc agaacatcat cccgtcttct accggtgctg
    18721 ctaaagctgt aggtaaagta ctgccagaac tgaatggcaa actgactggt atggcgttcc
    18781 gcgttccgac cccgaacgta tccgtagttg acctgaccgt tcgtctggaa aaagctgcaa
    18841 cttacgagca gatcaaagct gccgttaaag ctgctgctga aggcgaaatg aaaggcgttc
    18901 tgggctacac cgaagatgac gtagtatcta ccgatttcaa cggcgaagtt tgcacttccg
    18961 tgttcgatgc taaagcgggt atcgctctga acgacaactt cgtgaaactg gtatcctggt
    19021 acgacaacga aaccggttac tccaacaaag ttctggacct gatcgctcac atctccaaat
    19081 aagttgagat gactctgtga tctaaaaaga gcgacttcgg tcgctctttt ttttacctga
    19141 taaaatgaag ttaaaggact gcgtcatgat taagaaaatt tttgcccttc cggtcatcga
    19201 acaaatctcc cctgtcctct cccgtcgtaa actggatgaa ctggacctca ttgtggtcga
    19261 tcatccccag gtaaaagcct cttttgcatt acagggcgca caccttctct cgtggaaacc
    19321 tgcgggtgaa gaagaagttc tgtggttaag taacaacaca ccgttcaaaa atggcgtcgc
    19381 cattcgcggt ggcataccgc tttgctggcc gtggtttggt ccggctgcac aacaaggtct
    19441 gcctgcgcac ggttttgccc gcaacctgcc gtggacgctg aaatcacatc gtgaagatgc
    19501 tgatggtgta gtgctaactt ttgaattgac acaaagcgaa gagacgaaaa aattctggcc
    19561 gcacgacttt acgctgttag cgcatttcca cgtgggtaaa acctgtgaaa tcgatcttga
    19621 agcacacggc gaatttgaaa ctacctctgc tctgcatacc tactttaacg tgggtgatat
    19681 cgctaaggta agcgtcagtg gactaggcga tcgcttcatt gataaagtaa atgacgcgaa
    19741 agaagatgta ctgaccgacg gtattcagac cttccctgac cgtaccgatc gcgtgtatct
    19801 gaatccgcaa gattgcagcg taattaatga cgaagcgctg aatcgtatta tcgccgtagg
    19861 ccaccagcat catctgaacg ttgtaggctg gaacccagga ccggcgcttt cagttagcat
    19921 gggcgatatg ccggatgatg gctacaagac ctttgtttgt gtagaaacgg cttacgcttc
    19981 agaaacgcaa aaagtcacca aagagaaacc tgcacatctg gcgcaatcca ttcgcgttgc
    20041 gaaacgttaa tttgcgttaa tattgtgtgc cgggtgcgat gcatccggca cgtatcaaca
    20101 tcacaccata tccagcgcag tttttccttt tggtgccgga tacgctttat ccagcataac
    20161 taattccgct gacgaaagtt caacctcaag cacagccgca ttttgttgga catgtgcgac
    20221 agttgccgct tttgggatcg ccatcacacc ctgatggctg attacccacg ccaacaatac
    20281 ttgtgccgca ctgatattgt gagcatgtgc aatttcgttg actaccgcgt tttttaacag
    20341 tccattgcgc aaccgcccgg cctgagctaa tggactgtaa gccatcaccg gcatctgctg
    20401 tttctggcac cagggaagta gatcgtactc aatccctcgt gaaccgagat ggtaaagcac
    20461 ctgattagtg gcacactgat ttccccccgg cagttgccag agttcctgca tatcggcata
    20521 atcaaggtta gaaacgcccc agcggcggat ttttccctgg gcgatcaatt tttccatcgc
    20581 tgcgacagtc tcttcgagag cgaaactgcc agaccagtgt aataagtaga gatcgagata
    20641 atcagtattg agacggcgta aactggcttc gcatgcattt atcgcttttt gcccgccagc
    20701 attccacgga tagactttag agacgagaaa gacgttctca cgcagaccga ttaatgcttc
    20761 cccaaccact ttttcggcac cgccatcggc atacatttcg gcggtatcaa tgagggttaa
    20821 accgagttca atgcccgcgc gtagtgcagc aacttctgtt ttacgctgac tggcatcttc
    20881 gcccatatac catgttccct gccctatggc tggcagtgag acatcgccac taaattgaat
    20941 cattttttgt tgcattgctt cctcccaggt attgcaccac cgtaatgcaa aacagggcat
    21001 tacgccctgt ttttatgcac aaaatgccct ggaaaaatgc attatcagaa tttgtaggtg
    21061 atcccggtag aaatcaggcc agtccaggat ttatccacca ttgggctgtc agtaacttca
    21121 tcagacagac gggtgtagcg cgcgataccg taaacactcc agtcaccgag gaagttgtag
    21181 ctggcgctca gctccaggta agggctccag ctgtcgttcg ggttatagcc atgcagaccg
    21241 ctgcgagcgg actctttgcg cgatacgcca taatagtatt cgttctggtt ttcgctgttc
    21301 cactgcacac caatacccgg agtcacggtc aggccaccgt tggtgtaacg atacaaccag
    21361 gccatatccc agacgatgcc gttgctgtta tccagggtat cgccagccag ggtggtacgc
    21421 aggtaaccgt actgggtaaa gtgagcataa gacagaccag ccatcatggt gctcttacgg
    21481 tcatccaggt gacgcatttg gtgatcgcca ctgtctttag ctttgaagta aagcggcgac
    21541 cagtaagcgg taattgaaag tttgtccgtt gcgtcattcc acaggtagta accaccacct
    21601 aagccacgga accagaagtt atcgccttca tagttgatta ccggtactgg gtaaacatcg
    21661 gtatcgtaat ctttatatgg gtgttcaacg acacctacgc ctgcgcccag ggaaaattta
    21721 ccttcagcgt gcgctacgcc tgcagacgtt gcgataagca ctccaagtgc cagaagtttg
    21781 agtttggtca caattaatca ttccttaaac aaatgtttag cgggcgacaa agtttacccg
    21841 tcaatacatc gaaacccaac ctttttacgt tttcattttt tatagtaact gtttaatttt
    21901 cctgacgcag atgacaccac gcttacagcc aaatgaattt agcgttaccg gcgaccctat
    21961 tatttacatt aattatgcaa aatttatgga tgagttgttg atatgccatt gaaattaaga
    22021 aagccgtgca ggcaagtttt ccatttgcca tctacgctta attttgaagg tgtatcaccg
    22081 ggcacgttgt tctcatcgtc aataaaatgg catgagagtt gctgtgtttt agcaagagac
    22141 gtcgttcagt ttacctcttc cgggagcctc aactattcat atgaacggct cttaacctgt
    22201 gctaaaaaac gaaaggacgg cataccatga atatattcga tcactatcgc cagcgatatg
    22261 aagctgccaa ggacgaagag ttcacactgc aggagtttct taccacttgt cggcaagatc
    22321 gcagtgctta tgccaacgcg gctgagcggc tattgatggc tattggtgag cctgtcatgg
    22381 tcgatacagc ccaggaaccc agactttctc gactcttttc taaccgggtc attgcacgtt
    22441 atccggcgtt tgaagagttt tacggcatgg aagacgcgat tgaacagatt gtctcttatc
    22501 tgaaacacgc ggctcagggg ctggaagaga agaaacaaat cctgtatctg ctggggcctg
    22561 tgggtggggg taaatcatcg cttgctgagc gactgaaatc attaatgcag ctcgtaccga
    22621 tttatgtatt gagcgccaac ggtgagcgta gcccggtcaa cgatcatccg ttctgtcttt
    22681 tcaatccgca ggaagatgcg cagattctgg aaaaagagta tggcattcct cgccgttatc
    22741 tcggcaccat catgtcgccg tgggcggcaa aacgcctgca tgaatttggt ggcgatatca
    22801 ctaagttccg ggtagtgaag gtctggccgt caattctgca acaaattgct atcgccaaaa
    22861 cagaacccgg cgatgagaac aaccaggaca tctccgccct ggtagggaaa gtcgatattc
    22921 gtaaactcga acactacgcg cagaatgacc cggacgccta cggctattcc ggtgcgctgt
    22981 gccgcgccaa tcaggggatc atggaatttg tcgagatgtt taaagcaccg ataaaagtgc
    23041 tgcatccctt gttaaccgcc acccaggaag gtaactacaa cgggacggaa ggtatctccg
    23101 ccctgccgtt caacgggatt attctcgccc actcgaacga atccgaatgg gtcactttcc
    23161 gtaataacaa aaacaacgaa gccttcctcg accgtgttta catcgtgaag gtgccgtatt
    23221 gcttgcgcat ttccgaagag atcaaaatct acgagaaatt gcttaatcac agtgaattga
    23281 ctcacgcccc ctgcgcccct ggcacgctgg aaacgctgtc acgtttttct attctttcgc
    23341 gtctgaaaga gccagaaaac tccagcattt attcaaagat gcgggtttat gatggtgaaa
    23401 gtttgaaaga cactgatccc aaagccaagt cgtatcagga atatcgtgac tacgccggtg
    23461 tcgatgaagg gatgaacggt ctgtcgacgc gttttgcgtt taagatcctc tcccgcgtgt
    23521 tcaacttcga tcatgtagaa gtggcggcaa acccggtcca tctgttctac gtcctggaac
    23581 agcagatcga gcgcgagcag ttcccgcaag agcaggcaga acgctatctg gagttcctga
    23641 aaggttatct gatcccgaaa tatgccgagt ttatcggcaa agagatccag acggcctacc
    23701 ttgaatccta ttcagaatat gggcagaaca tttttgaccg ttatgttacc tacgcggatt
    23761 tctggattca ggatcaggag tatcgcgatc cggataccgg gcagctgttt gaccgcgagt
    23821 ctcttaacgc cgagctggag aaaatcgaga aaccggcggg gatcagtaat ccaaaagatt
    23881 tccgcaacga gattgttaac ttcgtactgc gagccagagc caataacagc ggacgtaatc
    23941 cgaactggac cagctatgaa aaactgcgca cggttattga gaagaaaatg ttctctaata
    24001 ccgaggagct gttgccggtt atttcgttta acgccaaaac gtcaaccgac gagcagaaga
    24061 aacacgacga ctttgtcgac cgtatgatgg aaaaaggcta cacccgtaaa caggtgcgtt
    24121 tactgtgcga atggtatttg cgcgtacgta aatcgtctta acaaccctgg cccggtacac
    24181 atgctaccgg gcctacaacg acagcgaacc gtgggccaga gaagcggcaa cacaggcgta
    24241 gcgtaccgtt ggcaaatgta gtacgggggg catatgacct ggtttattga ccggcgtctg
    24301 aacggcaaaa ataaaagcat ggtgaatcgc cagcgtttct tacgccgtta taaagcgcaa
    24361 attaaacagt cgatctccga ggccattaat aagcgttcgg tgactgacgt cgacagcggc
    24421 gagtccgtat ccattcccac ggaagatatt agcgaaccga tgtttcatca ggggcgtgga
    24481 ggtctgcgcc accgcgtgca tccgggtaat gaccatttcg tccagaacga ccgaattgaa
    24541 cgtccccagg gtggtggcgg aggttccggc agtggtcagg gccaggccag ccaggatggt
    24601 gaaggtcagg atgaatttgt ctttcagatt tcgaaagatg agtatcttga tctgctcttt
    24661 gaagatttgg ccttaccgaa tctgaaacaa aaccaacaac gccagctgac cgaatataaa
    24721 acgcatcggg cgggttatac cgctaacggc gttccggcca atatcagcgt tgtacgttca
    24781 ttgcagaact cactggcgcg gcgcacagcc atgacggcgg gcaagcggcg ggaacttcat
    24841 gcactggaag agaatttggc catcatcagc aacagtgaac ctgcgcaact gctggaagag
    24901 gaacggctgc gcaaagagat tgctgaattg cgcgccaaaa ttgaacgcgt tcctttcatt
    24961 gacactttcg atttacgtta caagaactac gaaaaacggc ccgatccctc cagccaggca
    25021 gtgatgtttt gcctgatgga cgtttccggt tcaatggatc agtccactaa agatatggct
    25081 aaacgttttt atattctgct gtatctgttc ctcagcagaa cgtataagaa cgtggaagtc
    25141 gtatacatcc gccatcatac ccaggcgaaa gaagtcgatg aacatgagtt tttctactcg
    25201 caggaaactg gcggcaccat tgtttccagc gccctaaaac tgatggatga ggtagtgaaa
    25261 gagcgttata acccggcaca gtggaatatt tacgcagccc aagcatcaga cggcgataac
    25321 tgggccgatg actctccgct ttgccatgaa attctggcga aaaaaatatt acccgttgtt
    25381 cgttattaca gctatatcga aattacacgt cgcgcgcatc agacactgtg gcgagaatat
    25441 gagcatctgc aatctacttt cgacaacttt gcgatgcagc atatccgcga ccaggatgat
    25501 atttatccgg tgttccgtga actgtttcat aaacaaaatg caacagctaa agactaaaac
    25561 tgtcagccgg gtcattatcg cctggctgat ttttagctca ctgtaaatta tctcatttat
    25621 tacatacatt agcttacaat cgctttaaat atgacagcat aacctttaca caatttagtt
    25681 ccagaaaaca atcattcgga aaaatgattc ggtcaacacg tatttccctg gggttattct
    25741 ttaattattt tttatcgtta acgaaaattg atcctggtca aaactatata tctctgccat
    25801 caataaaatc cagcactcac attgctctcc tttttatggt ttctatgggt acacaaaaat
    25861 taaaagctca aggctttttt attttcagct tattgctgac gttaatttta ttttgcatta
    25921 ctaccttata taacgaaaat acaaatgtaa aactcatccc acagatgaat tacctgatgg
    25981 ttgttgtggc tttgtttttc cttaacgccg tcattttcct tttcatgtta atgaagtatt
    26041 tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattttta agtggcctta
    26101 tctatttagt tgaaaccatt gtaattatcc ataaaccaat caacggcagt acactgatcc
    26161 agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt ttcatttgtt
    26221 taacctcgct ggcgctcttt tgttatggga aagacaacat ccttgacaac aataagaaga
    26281 aaactggcat tctgttgctg gcgctgattc cttttttagt ttttcccctt ctggcacaca
    26341 atctgagcag ttataacgct gactattctt tgtatgtcgt cgattactgt ccggacaacc
    26401 atactgcgac ctggggaatc aactatacaa aaatattggt ttgtttgtgg gcatttttac
    26461 tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta atagcattat
    26521 tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag tataattaca
    26581 ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt gtgtcttttc
    26641 tgatttataa catttttcag gaactgcaac tctccagcaa actggcagtt catgatgtgc
    26701 tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta ttgtcgcgac
    26761 ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa cgcatcaatg
    26821 cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat attatccagc
    26881 aaagcatccg ccccgatgat attttagcgc gactggaggg tgaggtattt ggcttgctat
    26941 ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt aaaaatgtcg
    27001 aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc atcagtattg
    27061 gcacggtttt ttcaacgggt gacacgcata atatctcgct tgtcatgacg gaagcagata
    27121 aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat catatttaag
    27181 tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt attactatta
    27241 cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc cctgtgccag
    27301 gccgcaatcg aactttatct ggttttctcg tttcactaac cgaaggagtg ccatttatca
    27361 tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt gtgctgacat
    27421 cttccttcct tatttttgaa cttgtcgcca gtgacagagc aatgagtgcc tatctgcgct
    27481 atatcgtgca gagagcagac tcctcctttc tttatgataa gtatcagaat cagagtattg
    27541 ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca gaacagcggc
    27601 gcgccatctg cgaggctttt gagtctgcca ataataccca tggcttaaac ctgactgccc
    27661 ataaataccc tggcttacgc ggcacactac aaaccgcatc cactgactgc gacacaattg
    27721 tggaagctgc tgcactatta cccgcttttg atcaggcagt ggaaggcaac cgccaccagg
    27781 atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat ctcgatctca
    27841 atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg atgaataact
    27901 ggtccttcct gcagtcagga agtattggta tcgatcgcaa agatattgaa aaagtattta
    27961 ccgggcgtac cgtattatcg agcatttacc aggatcagcg tactaaacag aacgtgatga
    28021 gtttgctgac gccggtatat gtcgcagggc agctaaaagg gattgtgctg ctggatatta
    28081 acaaaaacaa tctgcggaat atcttttaca ctcatgaccg ccctctcctc tggcgttttc
    28141 tcaatgtcac gctaaccgat acagattcgg ggcgcgacat tatcatcaac cagagcgaag
    28201 ataatctgtt ccagtatgtc agttacgtcc atgacttacc gggcggcatt cgtgtctcgt
    28261 tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg ttctggattt
    28321 tgacggcgtt aattttgctg aatatggtgc ggatgcactt ccgcttatac caaaatgtgt
    28381 cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt ttaacccctg
    28441 aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg tttattgcta
    28501 ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag ggggatttag
    28561 cgattacgtt attagctcag gcgattaaac agtcgattcg taaaagtgat tatgccatcc
    28621 gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa attgcagcac
    28681 aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa gagatcggct
    28741 tctcttccgg tatttacgcg atgaaagaaa acgatacgtt acatgatgcg tataaagctt
    28801 ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga taatcttctg
    28861 tggttgtttg cttgtaatct caggagcatg aaatgactga aatggctaaa ggaagcgtga
    28921 cgcatcagcg gttaatcgct ttattatcac aagaaggtgc taacttccgc gtcgttaccc
    28981 atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct ttaggccagg
    29041 gggctaaggc actggtatgc aaagtcaaag gcaatggcgt aaatcaacat gttctggcga
    29101 tcctcgctgc cgatcaacag gccgatctga gccaactcgc cagccatata ggcggattac
    29161 gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac aggctgtgtc ttcggcgcga
    29221 tccccccttt cagcttccat ccaaaactca aactggttgc cgacccccta ctcttcgaac
    29281 gatttgatga aatcgccttc aatgcaggca tgctggataa atccgttatt ttgaaaaccg
    29341 cggattattt gcgtattgcg caaccagaac tggtcaattt ccgccgcact gcgtaactag
    29401 cctgtccgtt caataagcag aacggacaaa accagcacaa ctgcaatggc aaaaaacgat
    29461 gacgtgataa tcagtgtctc gacgaacatt tgatcgttca tagtatgccc ccgggagata
    29521 gctgttttca cgttattgtt aacgtacaca aatggcagtt tgatgacagt tcgtctgatt
    29581 ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt aaagtaagca
    29641 ggctcattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct gatcctgctc
    29701 gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc aattctggtg
    29761 ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga aaaacagggg
    29821 ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcgc cagcgggacg
    29881 ctaccgccct caaccctgat tcactcgttt ctgaactgga aatcgctggt ggcaattgca
    29941 gtcggcgtta ttgtctcctg gctggggggg cgcggcgtga cgttaatggg cagccagccg
    30001 caactggtcg ccgggctgct ggtgggcact gttttaggcg tggcgctgtt tcgcggcgta
    30061 ccggttggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa acagtagtta
    30121 atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata gtaataaagg
    30181 ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg cctttcacta
    30241 atccctgaag cgccataatc agctgtaact gctgacgcca ctggcgaaaa ctcaaccccg
    30301 tctctttcac tatcagccgg gcaagattgc gttcactcat cgcaaaaaag ccagcccatt
    30361 gccccaatga gccccactcg acgggctctt tcgccatcat ctccaccatc gtgcggattt
    30421 taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg agttcatcaa
    30481 acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc gtcctgtttg
    30541 ccagggttaa aattaattcc cggcataagg gcgatatttt caatgtgcaa catgttgtcg
    30601 gcattgtcac tgcgctgggc tcgataaaca gaaaacagag ttcagcgtta gctgtcactt
    30661 gattactatg tttcaccccg cccggtatcc aaaccgcata ttgtggcggc accatccaca
    30721 aagcattttc caccgtacag gtaattgcac catgtagtgc cagtatcaac tgccctttgc
    30781 ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa aatgcgaccg
    30841 cggcttcatg gtgtcggtcg ggttcatagc cattgagatt caacctgtgc atcattttgt
    30901 ccgaatttag cgatattttg tcattttagc ttgattcaac ataacaataa aaacggtaag
    30961 gtacagcctc gtttgtaaca atgagaagca tatgacctgt tcaacttcat taagcggcaa
    31021 aaacaggatt gtccttatcg ctggcattct gatgattgcc acaacattac gcgtcacctt
    31081 taccggcgca gcaccgttac tggatacgat tcgttccgct tactcgctaa cgacggcgca
    31141 aaccggctta ttgaccaccc tgccattatt agcctttgcg ctaatctcac cgctggccgc
    31201 cccggtggcg cgacgttttg gtatggaacg tagcctgttt gccgcgttac ttttgatctg
    31261 tgcaggtatc gcaattcgct ctctcccttc gccttactta ttatttggcg gtacagcgat
    31321 cattggcggt gggattgcat taggcaatgt cttactgcca ggattaatta aacgcgattt
    31381 ccctcattcc gtcgccagac ttaccggcgc atattccctg acaatgggag ctgcggcggc
    31441 actgggatcg gcgatggtag tgccgctggc tttgaacggt tttggctggc aaggcgcgtt
    31501 gctcatgctg atgtgttttc ctctgctggc tcttttttta tggctgccac agtggcgaag
    31561 tcaacaacat gcaaatttga gtacctcacg cgccttacat actcggggta tctggcgttc
    31621 gccgcttgcc tggcaggtaa cattgtttct tgggatcaac tcactggtct attacgtgat
    31681 tattggctgg cttccggcga ttctcatcag tcacggctat agcgaagcac aggcgggttc
    31741 actgcatggt ttgctgcaac tagccacagc agctcccggt ttgctgatcc cacttttctt
    31801 acatcatgtg aaagatcagc gtggtattgc agcgttcgtt gccttgatgt gcgcagtggg
    31861 cgcggttggg ctctgcttta tgccagcaca cgcgatcacc tggactctgc ttttcggttt
    31921 tggttccggc gcaacaatga tactggggtt gacgttcatt ggtctgcggg caagttctgc
    31981 gcatcaggcg gcggcactct cggggatggc acaatccgtc gggtatttgc tggcagcctg
    32041 tgggccgccg ctgatgggta aaatacacga tgctaacggt gactggtccg taccactgat
    32101 gggtgttgca atactttcac tactgatggc gattttcgga ctttgcgccg ggagagacaa
    32161 agaaattcgc taatatccgg tgctatagtg acgtgacaaa tcatgcgtga aagggagaac
    32221 aaatacgatg aatattcagt gcaaacgcgt ttatgatccg gctgaacaga gcgatggtta
    32281 tcgcgtactg gtcgaccgcc tctggccacg cggtatcaaa aaaaccgatt tagcccttga
    32341 tgaatgggat aaagaaatca cgccgtcaac ggagctgcgc aaagcctttc acggcgaagt
    32401 cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac acgagcaaga
    32461 aggaaagcgg ctggcagaca tcgccaaaaa acagccgctg accctgctct actcagcaaa
    32521 aaacaccacg cagaaccatg cgctggtgct ggcagactgg ctacgtagct tgtgatttta
    32581 gtacagcatc cggcggttat ttttcaccag ccggatggtc ccgtcgccac aatgcccatt
    32641 catcaatcgt ttcaccgccc ggtaatttgc aattgttgct gaccccttgc gctgtctgca
    32701 caggaatgag cgtcccgccc ttctgctggc aatagaccga cgccggattt gccataccaa
    32761 tctgcggcgg tttaggtgct tctggctgag aaggggttga acaaccagcc aggaccagca
    32821 agcaaggcag aacaaaactg ataattttca tttattgatc tcacatattt atccaggctt
    32881 agagtatcgc ggtatcgttt tgttttgcag cactattttt attacattca ctcaaaacat
    32941 attacgcctt gtttcatctt tgttgatgat gttttatcat gcctgcaaag attaaataat
    33001 cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag atcagattat cgcccgcgtc
    33061 tcgcaatccc ttgccaaaga acagtcactg gaaagtctgg ttcgacagct tctggagatg
    33121 ctggaaatgg tcactgatat ggagtcaacc tacctgacca aagtggatgt cgaagcgcgc
    33181 ctgcagcata taatgtttgc ccgtaacagc cagaaaatgc acatcccgga gaattttacc
    33241 gtctcgtggg attactcgtt atgcaaacgc gccattgatg aaaactgctt tttcagcgat
    33301 gaagtccccg accgttgggg cgactgtgtt gcggcacgca atcttggcat caccacattt
    33361 ctgagcacgc caattcactt accggatgga tcattctatg gcacgctttg cgccgccagc
    33421 agtgagaagc gccagtggag tgaacgcgcg gaacaggtct tacagttatt cgccggactg
    33481 attgcacaat atattcaaaa agaggcactg gttgaacagc tgcgcgaagc taatgctgcg
    33541 ctgattgcgc aatcgtatac cgactcgtta accgggctac cgaatcggcg ggcgattttt
    33601 gaaaatctga cgacgctgtt ttctctcgcc cggcatctta accataagat aatgatcgcg
    33661 tttatcgatc tggataactt caaattaatc aatgatcgtt ttggtcataa tagtggcgat
    33721 ctgtttctca ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg cgaagttatt
    33781 ggtcgtctcg gcggtgatga gtttttggtt gtttcactga acaacgagaa tgcggatatt
    33841 tcgtcgctgc gagaacgtat tcaacaacaa atacgcggag aatatcactt aggtgatgtt
    33901 gatttgtatt atcctggtgc cagtcttggc atagtagaag tcgatcctga aacgaccgat
    33961 gcagacagtg ccctgcatgc tgccgatatc gcgatgtatc aggagaaaaa acataaacag
    34021 aaaacacctt ttgtcacgca ttcagcgcta cattcctgag gcgtattcac atccttttga
    34081 ttggtgataa catgcgaatc ggtattattt ttccggttgt aatctttatt acagcggtcg
    34141 tatttttagc atggtttttt attggcggct atgctgcccc gggagcataa agatgaaaaa
    34201 aacaacgatt attatgatgg gtgtggcgat tattgtcgta ctcggcactg agctgggatg
    34261 gtggtaacgt cacatctaaa aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt
    34321 acgcgttaac ttttaatctt cctgtagata aatagcacaa caatcgcacc aataacggca
    34381 accacgaagc tgccaaaatt gaagccatcg actttaccaa agccaaacag cgtgctgatc
    34441 catccgccga ctacggcacc gactatcccc agcaggatag tcataaagaa tccaccacca
    34501 tctttacctg gcatgatcca cttcgccaga ataccggcaa taagcccaaa aataatccat
    34561 gacagaatgc ccattgtttc ctcacttatc tgttttgcat tagcgggtta gtcgctgata
    34621 aaaagcatag cacaacatca ggagggtaag atttgtgacg agcatcacgg aggaattttt
    34681 tgcgatggcg cagaaattgc gccatcaacg atcagtgata attaccaacc acaaacatca
    34741 tgttcgtttt ccgtgtcata agaacgcacg gtattcacca gatcttttat cacttcagcc
    34801 gccacttctg gcaccagcaa agtcatcggc gtctctgttt cataatcgac agaaacgcca
    34861 ttgctgttat tggtgacggt cacggtatac gttgctttgc ccatgattca tttcccgtta
    34921 tgaatgactt tccgttgttg cgcgccttcc atcaggactt caggagcaac gaagaagtca
    34981 atgttgaaat aagtatcgtc agtcatggct tcaatgttgt gccacttttc tggagggaac
    35041 accgcaaact gccccgcttc gataaggatc acctgatcag gctctgcact gtgttcatca
    35101 gcgtagccga gatatttgac cgccccatgc ataacggaaa ggcgtgggta aacccctggg
    35161 cgcgttcctt tatcaagatg acgttcgaat attccggcag gcgcagtttg tttattccag
    35221 aaaggcgttg agcgcgtatg aatataattc tgtgggattt gcagcatcct tttccctcct
    35281 tcggtgaatg cgctgaaaac ggtttattcc agccgtttca gggtacgcct gataacttgc
    35341 attttaaata ccatttattg gttacttttt agcaccgtat cagagaagaa tcagggagga
    35401 ttacaggtgg gaagcccgcg gcaacgcggg ctgaaagcat caggattgca gcgtcgccag
    35461 tcgggcggcg aaacccacga acatcaaacc aatcagtgag ttgcccactt tagccagttt
    35521 ctttttggta cgtatgtact gcgtgacaaa agccccagaa ataatcagaa agctcaaata
    35581 gcagaaactc accagttcca gcgtcgtcgc cagaataaag aatgaaattc ccgtatgtgg
    35641 ggcattaaca tcgataaact gtacgaaaaa cgacacatag aacaaaatgg ctttcggatt
    35701 agtcaggctc aaaattaacg cgcgtttaaa aatggcaccg tattggggct catcggattt
    35761 ggtctcgcta tttttacctt tcagggtcgc gtagagaatt ttactcccca gatagagcaa
    35821 ataaaacgca ccaagataac gtacaatgtt gaataatatc ggggtggtct taattaatgt
    35881 cgccactcca gcccatgcca gaaacatcaa taccgcatcg ccaataaata caccacaggc
    35941 cgcaagataa ccgcctttca taccgctact gacgctattt ttgagtacaa acagggtatt
    36001 tggccctggc accaacacaa taaaaatggc cccaaccaga taggtccagt aattcagaac
    36061 cccgtattca gcgaacacat taacctcttt aattatcttt cgatcatgcg cgattaaaag
    36121 tgaatatgct aaccaatctg tagcggctta gaaaggagaa aatcaggttt taacccgata
    36181 tcaacccgat aattgaatca ttaatcaggc atactccagt gaaaaattcg ggtagtgctc
    36241 ggcaaaatac tggcgtaaaa attctaccgt tatccgcact ttcgctgaca tcgccagcct
    36301 tgaaacataa acggcccaga cattcgctgg ctggtaatat tcgggtagca cttgtactaa
    36361 atgaccactg gcgggaaaca gcctggcgac atcgattttt acgtggtccg gaaaaacacc
    36421 gaaggcgaat attcctcgcc aggcggtaga gtgaatgaag gaacagagaa tgaagtcgtc
    36481 attcaggaat cggtatttac tcgtcgtggt gtcgagcgca ttttgcgtta agctcaaata
    36541 agttcatttg aaccgcctgc tgttggattt gtagtttgcc gaagccggac tgagcgcgcc
    36601 cagtccggct tcggaagatt ttactgtgga tattcctgca ataaattgtg taatgcttcc
    36661 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    36721 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    36781 atcttgtgcg tttcgctgac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    36841 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgcc
    36901 tgcgcttttt ccaccagcgc cagcaacgtt ttaccgtgga agcggatttc gctaccagat
    36961 aagcccaggc tgtccagttc gcccagactt cccggcatat aacgcgctac cgaccacaaa
    37021 tgctcttcac gcacgacaaa gttcaccgcc agatcgcgct ctcgcgcctt gcgcaatcgc
    37081 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaactgcca ggcattggtg
    37141 atatcacgcc aggcgtcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    37201 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagctttgcg
    37261 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    37321 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    37381 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    37441 gtgtcaatca agggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    37501 ctgcctgcat gaagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    37561 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcaaa aagttgaatt
    37621 aaacccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatccag ggctatcgcc
    37681 ggaaaggcac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    37741 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    37801 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    37861 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    37921 ttcggtactt tgtagcccgt gagctgacgg cggcaaaagg tcaccagtga ctcttcggta
    37981 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    38041 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    38101 ttgggataaa cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    38161 aggaatcctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    38221 atttcatcgg tagcatccgg acgctgccag taacccagca tcacctgcgg tcctttgaca
    38281 caaagctcac ctggttgacc cggtggtact tcgttgtcgt cgtcatccac cagtttggct
    38341 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atacgggtta
    38401 acgctgacca gcggcgcgca ctcggtcagg ccataacctt ccagcagata ctgtccagtc
    38461 agtttaaccc aacgttctgc aaccacttgc tgaacaggca ttccaccgcc tgcggaaaga
    38521 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    38581 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    38641 atatcgcgcg ggttagtgat aagcaggttc tgccccccta gttcgataaa cagcaggcag
    38701 ttaatggtca gtgcaaaaat gtgatacagc ggcagcgccg tcaccaccag ctctttgccc
    38761 ggatgcaaca gcggaccata ggtcgcgtta acctgttcca ggttcgccag catattgcgg
    38821 tgagtcagca tcgcgccttt cgccacacca gtggtgccgc cggtgtattg caggaaagct
    38881 aaatcttccg gcaccagttc tggtttgacg tactgcatcc ggtagccgtt gtgcagggcg
    38941 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccagacgctt gatgtattta
    39001 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    39061 ataacgtgct gaacggcggt tttatcaacc actttttcca gagtgtgggc aaagttagac
    39121 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    39181 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    39241 ggatattgca ataagttagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    39301 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagtttgcg gaaggtcatc
    39361 acctccccca tattcacaaa cgcgggttga tcggcgtagc gcgcgaccga ctgctcaaac
    39421 atatctacca gagattgata acggtcaggg ttgatctccg tcggaacgtc cgcgggataa
    39481 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    39541 accccaagtg ataaacatac tgttaacata atattaactc atcttaccag cttgataatt
    39601 acccaaggaa aaggttgcga agcgcgtcac tatttatttt tatctttacc gtaagaatgc
    39661 agaaacagcg gaccggccgc tgtttctttt tctttaaaaa caagcaatta ttcagttaca
    39721 actgtttgta ctctcgcggg gccgggatta taccagcccc atccgccgta gccatagggc
    39781 cagccacgac cgccataaaa ccatggatca atcggctgag gcggcataat cacctgctgg
    39841 gttaaatgcc aacgtttgta acccgttact tgcatcacca taaatttata gggcgtatta
    39901 ccgattttgc cgtcaaccgc accggtgatt ggcccgacta ccgtcaccag ttgtccgcgg
    39961 aaatccaccg ggtccagaaa accgttcaca tcggcataaa tgcgaccgcg agaaggttct
    40021 cccagcgtcg gtctggctcc gctgtccagt ggtacggtgg caatttccag gcgtgttttc
    40081 ccttgctggt tttgtaccgc aaccactttg ccgccaaagc gagcctcctg accaacgtac
    40141 agctgcggcg cactcatcac ccgaactaaa tcttgttgcg gcgtgggact gctgccttta
    40201 atggcgtctg gcacagtaac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    40261 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    40321 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaact
    40381 ggttcagcat gttccaccgc caccgtttta ccctcggcaa acatctggca cgcaatcggc
    40441 agcatatctt ctgcagcggg cagtaacact tcgccatcga gcaaaaccag cccgctctct
    40501 ttaccgagat ccggccaggc ttgccagccc gtccctactg tcacccattc gccggaaagc
    40561 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgtgc
    40621 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    40681 atagctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccttgcgc catcgtcatt
    40741 agcgtggaga cgccaatcat tggtaactcc gcgccaagtg ccagtccttg cgcaatgcca
    40801 ataccaatgc gcacgccggt gaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    40861 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    40921 gtatgttcac gagggcaaag ctcaaaatga gcgttgacag taccgtcgtt ccacagggcg
    40981 acagagcagg cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    41041 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    41101 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgcg gcgctggcgg cagactggcg
    41161 agaaacgtcg cgccgtaagg gcgcatcacc agccgattat cgcaaatcac cagcacgccg
    41221 cgatcgtcgg cgtcgcgaat caatcgccct accccctgct tgagagtaat gaccgcatcc
    41281 ggcagttgta cttcatcgaa cggatcacca ccgcgcaaac ggcaatcttc catgcgtgct
    41341 tttaatagtg gatcatccgg cgaggtaaac ggcaatttgt cgataatcac caatgacaat
    41401 gtatcgccac gcacgtccac cccttcccag aaactgctgg ttgccacaag aagcgcgtta
    41461 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    41521 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc gtgcgaggta
    41581 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    41641 tgccgtgcgg aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    41701 tggcggctgt aatcgaaggg gctgggtaac agcaacgact ctgcctgttc aatgccaagc
    41761 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt aaagatccag
    41821 ctaccgggtt tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    41881 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtaacccgg ctgattaatc
    41941 tcttttagcc gtttgagtcg cgtgcgatat aacgtggcgc gttcaaatgc cgcatccagc
    42001 aaggcggaac gccccagcga aagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    42061 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    42121 taacctggct caccgagttg caggcgaaaa tcctgcgcac tctgggcaag acggtccgcg
    42181 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    42241 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcg
    42301 ggcagctggt gggcttcgtc gaagatcatg acgtccgctt ccgggatcaa ttcgccaaaa
    42361 ccgctctctt tcaccaccat atccgccaga aacagatgat gattcaccac caccacatcg
    42421 gcgtccatcg cttttttgcg tgctttgacc acaaagcaat ctttatacat cgggcagtca
    42481 ctgccgaggc agttatcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
    42541 acgctgacgc aggtgctgat atcgccatcg actgtttgat tagaccagga gcgcagcagg
    42601 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga
    42661 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacattgcc cgtgtatttc
    42721 aatgcctttg agaccgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
    42781 ccggtcgaga ttatgacttt ctttttcgcc cgcagcgcag gggccaggta agcgtaggtt
    42841 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    42901 acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgct
    42961 aactgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    43021 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcgacctc acattgtggc
    43081 agtctttgca gcacgaaaat gggagtataa tgaggacaaa atgatgacta tcgttcgtat
    43141 cgatgccgaa gcccgctggt ctgatgtggt aatccacaac aacacgctct actacactgg
    43201 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcgcagat
    43261 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    43321 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    43381 gggtcatgcg ccggtgcgct gcacggtaca agcaggtttg atgaacccga agtataaagt
    43441 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    43501 tttatcgaca atactggtcg gttttgcata aatcgaccag agattaagat attacagttg
    43561 atgctcctgc taattcatta tttattttta taatttcaat tttatctata aaactaacta
    43621 tataaataaa cataatcatg ccgttatgtt cgcccttcga ttagcatctc tggttttctt
    43681 tcttagctga acaatctggt tatagcgtga ggactgacca ttaattttct ccatcttact
    43741 ggataacaca ctatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    43801 ccatatcgtt ataatccctt tgatattaga tacaaattaa ggtcatatat agccttatcg
    43861 ataacatggt taattttaag gataagaata tgcctgctgt aatagataaa gccctggatt
    43921 tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa agtacggcga
    43981 agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct gatattacgg
    44041 cgcgagctga ccaggaaggc tggaaccctg gtttcacgga aaaaatggtt ggatgggcaa
    44101 aaaaaatgga gtctggtgaa cgctctgtga ttaaaaatcc tgaatacttt tcaacatata
    44161 tgcaggaaga actaaaagca ctggtttgag ttaaatttac atcagcataa atgggtcagg
    44221 acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaataat catcagcttc
    44281 tataacgaaa gcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa cgcgccacga
    44341 ttcgctcgcc ggaatggttg gcacgcagct cttctgccac cagcgcgatt gcctggccgc
    44401 tgctcatccc ttgtgccatc agttcctgga tccgctcgac ggctttttgc tgctgttcat
    44461 gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt ggcgctaatt
    44521 atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg ttatctcccg
    44581 ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc cgcttaagtc
    44641 acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccatat agccgctttg
    44701 atattgtggt cgccgagccg atttgcactt taaccacttt cggtaatgaa accgtagtgt
    44761 gtgaaggaga aaccagctca acaactaccg acgatccgct acaggtgctc cagcaggtgc
    44821 tggatcgcgc agacattcac ccagcgcata acgaagattt gccatttcag ggcggcgcgc
    44881 tgggattgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa attgcggaac
    44941 aagatatcgt tctgccggat atggcagtgg gtatctacga ctgggcgctg gttgtcgacc
    45001 accagcgtaa aacagtttct ttgctgagtc ataatgatgt caatgcccgt cgggcctggc
    45061 tggaaaacca gcaattctcc ccgcaggaag atttcgcgct taattccgac tggcaatcca
    45121 atatgacccg cgagctgtac ggcgaaaaat ttcgcaaagt acaggaatat ctgcacagcg
    45181 gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct ggcgatgaat
    45241 ggcaggcatt ccttcagctt aatcaggcca atcgcgcgcc gtttagcgct tttttacgtc
    45301 ttgaacaggc tgctatttta agcctttcac cagagcggtt tattctttgt gataatggtg
    45361 aaattcagac ccgcccgatt aaaggcacgc taccacgcct gcccgatctt caggaagata
    45421 gcaaacaagc agaaaaactg gcgaactcaa cgaaagatcg tgccgaaaat ctgatgattg
    45481 tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc aggttcggta aaagtaccag
    45541 agctgttcgt ggtggaacct ttccctgccg tgcatcatct ggtcagcacc ataacggcgc
    45601 aactaccaga acagttacac gccagcgatc tgctgcgcgc ggcttttcct ggtggctcaa
    45661 taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa ccgcagcgac
    45721 gcaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg gacaccagca
    45781 ttactatccg cacgctgact gccattaacg gacaaattta ctgctctgcg ggcggtggaa
    45841 ttgttgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa gttaataaga
    45901 tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt gatgattttt
    45961 tatcgcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccttaaat catcgtcagg
    46021 ctgccgtgtt aatccccatc gtccgccgac cgcaaccggg gttgttgctg actcagcgtt
    46081 cgattcatct gcgtaaacac gctggacaag tggcattccc tggaggcgca gtcgatgaca
    46141 cggacgcatc agttatcgcc gctgcgctgc gcgaagctga agaagaggtc gctataccgc
    46201 cttccgccgt tgaggttatc ggcgtgctgc cgcccgtcga tagcgtcact ggctatcagg
    46261 taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt gaagatgaag
    46321 tctccgccgt ctttgaaatg ccgctcgccc aggcattaca tctgggtcgt tatcatcctt
    46381 tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac gaacagtatt
    46441 ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt ggtgtgaaac
    46501 cctgactata cttatcttta catctacaaa acactacttg agacaatcat cgcaatatta
    46561 gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagcctg tcgccgcgtt
    46621 ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac cttgtcagga
    46681 gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc tcatcttccc
    46741 ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct
    46801 tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtca ctgacgggta
    46861 aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa cctgccaccg
    46921 tggatatcga cagtattccc ggttttattc gcgacgtaga agagcgcgaa cgtctgctgc
    46981 ttgcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt tttcataacg
    47041 gtaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc gatgaagtcg
    47101 tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa gaacactttg
    47161 gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc accgaactgc
    47221 tcgcgtactg taatgaaacc ggcttttcgc tgtctggtct cgctatgcaa aacgaactgg
    47281 cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa accatgcagg
    47341 catgtatcga tcgcgggatg aataccgaag gcgtactgcc aggcccgttg cgcgtgccac
    47401 gtcgtgcgtc ggccctgcgc cggatgctgg tttccagcga taaactgtct aacgatccga
    47461 tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa aacgccgccg
    47521 gtggccgtgt ggtaactgcg ccaactaacg gtgcctgcgg tatcgttccg gcagtgctgg
    47581 cttactatga ccactttatt gaatcagtta gcccggacat ctatacccgt tactttatgg
    47641 cagcgggcgc gattggtgca ctgtataaaa tgaacgcgtc tatttccggt gcggaagtcg
    47701 gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggcctt gccgagctgt
    47761 tgggcggtag cccggaacag gtttgtgtgg cagcggaaat tggcatggag cacaacctcg
    47821 gtctgacctg cgaccccgtt gcagggcaag ttcaggtgcc gtgcattgaa cgtaatgcca
    47881 ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc agtgcaccgc
    47941 gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac atgaacgcta
    48001 aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac taatacttct
    48061 tactcgccca tctgcaacgg atgggcgaat ttattcccgc ttcctcgtct gctgtaattt
    48121 tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta cagggtggcc
    48181 cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat gattgtttgt
    48241 acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat ttcacagcgt
    48301 aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga attagataaa
    48361 gtactgcttc ccctacaggc aggtagcgaa gtcttacttc cgctaattgg tctgccctgc
    48421 tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt gcgatccatt
    48481 ggcctggtgc aagacggcac actttattgc tccagcattt ttggttatcg caatgtgccc
    48541 gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct gacgaccgac
    48601 cgtgccctga taaaaggcag tccggttttg attcaatgga cgcctgcagc gggcagtagc
    48661 aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct ccttgaacca
    48721 caactgccgc aaatcagtag cgccagcctg acggtggaca gtcggcattt gctctatggt
    48781 aatgggctgg tagattccct tccgcaacct gaaaataatg aaaactatca ggtttcttcg
    48841 caacgctttc cttttaccat taacgttaat ggcccggggg ctacggcgct ggcatggcac
    48901 tatcttccaa cacaattacc gctggcggta ctgctaagtt tactggtggg ctacatcgcc
    48961 tggctggcga ccgcttaccg aatgagcttt tcccgcgaga tcaatctggg cctggcgcaa
    49021 catgagttcg aattgttctg tcagcctttg cttaatgcac gcagccagca atgtattggt
    49081 gtagagattt tgctgcgctg gaacaatccg cgtcagggct ggatttcacc ggatgtgttt
    49141 attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa
    49201 accattcgtc agcgccatgt tttcccaatg agtagtcagt ttcatgttgg cattaacgtc
    49261 gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc
    49321 gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt
    49381 gattaccgga ttgcccgcga gctgcatcgt aaaaacgtca aactggcgat tgatgacttc
    49441 ggcaccggca acagttcatt ttcctggctt gaaacattac gtcctgacgt tctgaaaatt
    49501 gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc
    49561 atcatcgcac tggggcaaag actgaatatt gaactggtgg cggagggcgt agaaacgcaa
    49621 gaacaggcga agtatttgcg cagtcatggc gtgcatattt tgcaagggta tttgtacgca
    49681 cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc
    49741 cggcataatg gacatatcac acccattatg ccgttacgtt aacgcttact catcttcatc
    49801 gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat tggcttcaat
    49861 gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa tgtgaccatt
    49921 tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac
    49981 atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc agccgtcacc
    50041 atcagtaatg atttccggcg tttcgtcagc atccgggaat tcacccgcaa tggcttccag
    50101 cacatccagc ggcgtgacca agccttgtac cacaccaaac tcgttggtca cgataacaaa
    50161 gctcccgcga gcacgacgca gcacgcccag caggttgatc ggatcgaggg tttcagggac
    50221 gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt ccagcgccac
    50281 cagcagttct ttagcacgta cgataccaat gatttcatcc agttcaccgc gacataccgg
    50341 gaacagactg tgcggtgaag agagcagttg ctcgcggatt tcatcgaccc cgagattagc
    50401 gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac gcgacgccag
    50461 cgtcagtacg ccgttaatca tgtaacgttc ttcttcggca aatgcacctt ccgggatcgg
    50521 catcggcatc gggttatcgg catcgtgctg aacattggcc tgacgtttcc cgcccatcaa
    50581 acgcaggatg gcatcggcag tacgcgctcg cagcggcaaa gtcgactggt gacgaataaa
    50641 gttgcgacgc gcaatctggt taaacacttc gatgatgatc gagaagccaa tcgcggcata
    50701 caggtaacct ttcggaatgt ggaaaccgaa accttctgcc accagactca gaccaatcat
    50761 taacaggaag ctcagacaga gcaccaccac cgtggggtgc tggttaacga atcgcgtcag
    50821 cggttttgat gccagcagca taactgccat cgcaatcact accgccgcca tcatcaccgg
    50881 caaatggtta accatcccta ctgcagtaat taccgcatcc aacgagaaga cggcgtcaag
    50941 gatgacaatc tgtgtgacga ccacccagaa actggcgtag cctttaccgt ggccggaatc
    51001 atgatcgcgg ttttccagcc gttcatgcag ttcggttgtt gctttgaaca gcaagaatat
    51061 gcccccgaac aacataatca ggtcgcgtcc ggagaaggag aaatccatga cggtaaatag
    51121 cggtttggtc agcgtgacca tccatgaaat cagcgacagc agccccagac gcataatcag
    51181 cgccagtgat aatcccagca aacgcgcttt atcgcgttgt tttggcggca gtttgtcagc
    51241 aagaatggcg atgaagacta ggttatcgat acccagcaca atttcgagaa caacaagcgt
    51301 gagtagcccc gcccaaatcg aggggtccat taagaattcc atgacaagct cctgcttaag
    51361 gaatagctat tcgacgccag aaataatgca ggcgtaacga caaaatgcaa acgaaaggtg
    51421 cggcataaag tgccagaaag gcaggcgtta aaaggcctga tgctgaaatg acgtcggtga
    51481 cgatccatac tgcgggctac tgccctatac tccatggttg ttaaacagga gttaaacata
    51541 tcagagacgc ctctgatttg gcaaagattt accttccttt gcaaacgaat gtgacaagga
    51601 tattttacct ttcgaaattt ctgctaatcg aaagttaaat tacggatctt catcacataa
    51661 aataattttt tcgatatcta aaataaatcg cgaaacgcag ggggtttttg gttgtagccc
    51721 ttatctgaat cgattcgatt gtggacgacg attcaaaaac acatctggca cgttgaggtg
    51781 ttaacgataa taaaggaggt agcaagtgac cattgctatt gttataggca cacatggttg
    51841 ggctgcagag cagttgctta aaacggcaga aatgctgtta ggcgagcagg aaaacgtcgg
    51901 ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg attgaaaagt acaacgctca
    51961 gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc gttgatacat ggggaggcag
    52021 cccattcaat gctgccagcc gcattgtcgt cgacaaagag cattatgaag tcattgcagg
    52081 cgttaacatt ccaatgctcg tggaaacgtt aatggcccga gatgatgacc ctagctttga
    52141 tgaactggtg gcactggcag tagaaacagg ccgtgaaggc gtgaaagcac tgaaagccaa
    52201 accgattgaa aaagccgcgc cagcacccgc tgccgcagcc cccaaagtgg ctccaactcc
    52261 ggcaaaacca atgggaccga acgactacat ggttattggc cttgcgcgta tcgacgaccg
    52321 tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa accaatgtct cccgtattat
    52381 tgttgttagt gatgaagtgg ctgcggatac cgttcgtaag acactgctca cccaggttgc
    52441 acctccgggc gtaacagcac acgtagttga tgttgccaaa atgattcgcg tctacaacaa
    52501 cccgaaatat gctggcgaac gcgtaatgct gttatttacc aacccaacag atgtagagcg
    52561 tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc ggtggtatgg cattccgtca
    52621 gggtaaaacc caggtgaata acgcggtttc ggttgatgaa aaagatatcg aggcgttcaa
    52681 gaaactgaat gcgcgcggta ttgagctgga agtccgtaag gtttccaccg atccgaaact
    52741 gaaaatgatg gatctgatca gcaaaatcga taagtaacgt attgtgttga ttatcattca
    52801 gttttcacac tttagtctta cgtaaacagg agaagtacaa tggagattac cactcttcaa
    52861 attgtgctgg tatttatcgt agcctgtatc gcaggtatgg gatcaatcct cgatgaattt
    52921 cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta tcgttcttgg cgatatgaaa
    52981 accggtatta ttatcggtgg tacgctggaa atgatcgcgc tgggctggat gaacatcggt
    53041 gctgccgttg cgcctgacgc cgctctggct tccatcattt ctactattct ggttatcgca
    53101 ggtcatcaga gcattggtgc aggtatcgca ctggcaatcc ctctggccgc tgcgggccag
    53161 gtactgacca tcatcgttcg tactatcacc gttgctttcc agcacgctgc ggataaggct
    53221 gctgataacg gcaacctgac agcgatttcc tggatccacg tttcttctct gttcctgcaa
    53281 gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt ctgttggtac cagcgaagta
    53341 cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg gtctgaatat cgccggtggc
    53401 atgatcgtgg tggttggtta tgcgatggtt atcaacatga tgcgtgctgg ctacctgatg
    53461 ccgttcttct acctcggctt cgtaaccgca gcattcacca actttaacct ggttgctctg
    53521 ggtgtgattg gtactgttat ggcagtgctc tacatccaac ttagcccgaa atacaaccgc
    53581 gtagccggtg cgcctgctca ggctgctggt aacaacgatc tcgataacga actggactaa
    53641 caggtgagcg aaatggttga tacaactcaa actaccaccg agaaaaaact cactcaaagt
    53701 gatattcgtg gcgtcttcct gcgttctaac ctctttcagg gttcatggaa cttcgaacgt
    53761 atgcaggcac tgggtttctg cttctctatg gtaccggcaa ttcgtcgcct ctaccctgag
    53821 aacaacgaag ctcgtaaaca agctattcgc cgtcacctgg agttctttaa cacccagcca
    53881 ttcgtggctg cgccgattct cggcgtaacc ctggcactgg aagaacagcg tgctaatggc
    53941 gcagagatcg atgacggtgc tatcaacggt atcaaagtcg gtttgatggg gccgctggct
    54001 ggtgtaggcg acccgatctt ctggggaacg gtacgtccgg tatttgcagc gctgggggct
    54061 ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt tcttcatcct gtttaacctg
    54121 gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt actccaaagg tatcgatatc
    54181 gttaaagata tgggtggtgg cttcctgcaa aaactgacgg aaggggcgtc tatcctcggc
    54241 ctgtttgtca tgggggcgtt ggttaataag tggacacatg tcaacatccc actggtggtc
    54301 tctcgcatta ctgaccagac gggcaaagaa cacgttacca ctgtccagac tattctggac
    54361 cagttaatgc caggcctggt accactgctg ctgacctttg cttgtatgtg gctactgcgc
    54421 aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg tcatcggtat cgctggttac
    54481 gcttgcggcc tgctgggact gtaagactgt tgtacactac cggggccttt tggccccgtt
    54541 tttttatctg gaggattaat gacaatcacg gacctggtac tgattctttt catcgccgca
    54601 cttctggcct tcgcgatcta cgatcagttc atcatgcccc gccgtaacgg ccccaccctg
    54661 ctggcaattc ctttgcttcg gcgtggtcgc atcgatagcg ttatcttcgt cggattgatt
    54721 gtcattctta tctataacaa cgtcacgaat catggcgcgc taataacaac atggttatta
    54781 agtgcactgg ctctgatggg tttttatata ttctggatcc gcgttccgaa gatcatcttt
    54841 aaacaaaaag gttttttctt cgccaatgtc tggtttgaat atagccgaat caaagcgatg
    54901 aacttgtcgg aagatggcgt gttggtgatg caattagaac agcgccggct gttaatccgc
    54961 gttcgaaata tcgacgatct ggaaaaaatt tataagcttc tcgtttcaac tcaataagtt
    55021 atgaatttag ccaaagctat gtttagtgta tttttaataa tcagacatag ctaaggctat
    55081 attacctctt cccttatttg ttatttattt taacgtttca ttgatatata aatctaaatg
    55141 aaaatcgtta tcaacaaagc aataaaataa tatattccaa cagttgtttt atattctcaa
    55201 aatatgttaa ggttgcgccc tcatttgggg agtagccaat ttccagattc cggaaatgta
    55261 cgtgtcaaca tactcgttgc aaaacgtggc acgtacggac tgaacacttt cagtcaggcg
    55321 agaccatatg cacattaatc gctatgcctg catgaacgcg aaaaccatcg cggcttgttg
    55381 ttcatgtttg catgtttgct gggggcgatg atgtgtttta tggatacccc ggtcaggacg
    55441 ttgtcatgaa tatcactgct actgttcttc ttgcgtttgg tatgtcgatg gatgcatttg
    55501 ctgcatcaat cggtaaaggt gccaccctcc ataaaccgaa attttctgaa gcgttgcgaa
    55561 ccggccttat ttttggtgct gtcgaaaccc tgacgccgct gatcggctgg ggaatgggca
    55621 tgttagccag ccggtttgtc cttgaatgga accactggat tgcgtttgtg ctgctgatat
    55681 tcctcggcgg acgaatgatt attgagggtt ttcgtggcgc agatgatgaa gatgaagagc
    55741 cgcgccgtcg acacggtttc tggctactgg taaccaccgc gattgccacc agcctggatg
    55801 ccatggctgt gggtgttggt cttgctttcc tgcaggtcaa cattatcgcg accgcattgg
    55861 ccattggttg tgcaaccttg attatgtcga cattagggat gatggttggt cgctttatcg
    55921 gctcaattat tgggaagaaa gcggaaattc tcggcgggct ggtactgatc ggcatcggcg
    55981 tccagatcct ctggacgcac ttccacggtt aataagaacg ctgccagagg tgaatattaa
    56041 agtccgtctg gcagtcgaac acttcttttg ctgccagtgt ttgccagact tctggcttcg
    56101 cacgccaggc aaacggcgtc atctgcaata atgcgacggc ttcatcaccg cgaagacgca
    56161 tcggataaca caactcatca ctctgctgta atgtaaaacc ttccagttgt tctgcatgag
    56221 gtgcatgaag atgtacttca ttgtaaatca gccccttcag ctccattaag tgtctcggtc
    56281 ccggcgtagc agtaatgacc caaccgccgg gcttcactac tcgcactaat tcttctgctt
    56341 tacacggcgc gtaaatacgt attatggcgt ccatactggt atcggaaaac ggcaaacggt
    56401 ggctggaagc gacacaaaaa gtgacctgcg gatagcgttt cgccgccgct tttatcgcta
    56461 ccttcgagac atccaggcca aacgtggtga tttcgggcaa cgcatcagca aatgcgtgtg
    56521 tgtaataccc ttcaccacag ccaatatcca gcaccgccgc ggccttttca tcaagccgtt
    56581 cccgcagttg gccgacaatt gcatcacgca gcggctgata atgtccggca tctaagaatg
    56641 cgcggcgtgc ttgcatcatt tccgcgctgt cgcccggatc acgtgaccgt ttatgctgaa
    56701 cgggcagcag attgacatac ccttctttcg ccatatcaaa ctgatgtcgc tggggacaga
    56761 tatagctgtt tttttcacgc gaaagaggct gatggcaaag tggacaggaa aacgacatga
    56821 caactccggc aggatactaa aggccgcaag tgtaacgcga atcatgcggc gggcaaatat
    56881 ccgaacgact ggagcgaaga taaaacaaag taaaaagcct cgcattcgac gaggctttat
    56941 agcgagattg aagcgtattc acacttcaga tcagtggatt cgatcagata gctgttacgt
    57001 taacagctgc cggacctttc tgaccgtcct gaatttcgaa ctcaacgttc tgaccttcag
    57061 ccagagtttt gaaaccatta ccctggatag cggagaagtg tacgaacaca tctttgctgc
    57121 catcagccgg agtaatgaag ccaaaacctt tagactcgtt gaaccactta acctgacctt
    57181 taatctttgc catttgaaaa attccttaga ttgttttctt cgcccgcagg cataacatag
    57241 ataaaactga cacattactg catgaggcac caatataagg ctcggcagag aagcggtatt
    57301 caacgtcaac gtgtttactc aggacttctt tactgaaaat gccacacata aacagaactg
    57361 tacctcgttt aacccgaaat ctgttatcac atacaacgtt aattatggca agccattttt
    57421 aaacatgtct cgatcagacg cacaaatctc gatacgtctt ccactttttt tgcacactta
    57481 tgcaacggaa tacgcgccga tatatcattg tgcttaacct tgccagttca ggcagatact
    57541 taaactggcg tattttctaa catagttcaa taagttcagt tgttccaggc cagtgggaga
    57601 agttattaca tagtgcgtgc aatatcacat tttttgctat gcaatgaata aaaagttata
    57661 tcactttttc tcataaaaca gtcagttaac ggctatcaa
//
LOCUS       DAZKHK010000012        56472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKHK010000012 DAZKHK010000000
VERSION     DAZKHK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.012"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..540
                     /locus_tag="ACHOS5_000780"
     CDS             <1..540
                     /locus_tag="ACHOS5_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775658.1"
                     /translation="LLNDINYFMTYQGESINLISLDHEYPESCLQNDTNTYLLGRVIF
                     LYNSMIYKFINCQEHETNNIHSAMINNLLQEVDIALGKINNIIDSRNISAPHELANIL
                     TREKILTTREKKRNLISLFDGFTLFHCVGMITFLIHYLRTPEEKVENIFMLYGADKNN
                     KLRRRLIYDALGIIQSQQE"
     gene            complement(728..1930)
                     /locus_tag="ACHOS5_000781"
     CDS             complement(728..1930)
                     /locus_tag="ACHOS5_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9775659.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(2196..2882)
                     /locus_tag="ACHOS5_000782"
     CDS             complement(2196..2882)
                     /locus_tag="ACHOS5_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFP9775660.1"
                     /translation="MSFMAFVTCLTIAFVAGFVVARKLTLRKVASSVIISKSQPVPSS
                     ATPPAAKASTRSYTPQKTYRDSRWQERDELFQGMVAEGILLEGKEWLITRCEKIYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VGVIELDDPSHERADRIIRDHWLDTIMTLARVPFVRASVTKLEEIPPVWDTRDKIRPL
                     VAATKSEQLH"
     gene            complement(3471..4259)
                     /gene="phoH"
                     /locus_tag="ACHOS5_000783"
     CDS             complement(3471..4259)
                     /gene="phoH"
                     /locus_tag="ACHOS5_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP9775661.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(4886..6157)
                     /gene="efeB"
                     /locus_tag="ACHOS5_000784"
     CDS             complement(4886..6157)
                     /gene="efeB"
                     /locus_tag="ACHOS5_000784"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP9775662.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTVESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(6163..7290)
                     /gene="efeO"
                     /locus_tag="ACHOS5_000785"
     CDS             complement(6163..7290)
                     /gene="efeO"
                     /locus_tag="ACHOS5_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP9775663.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(7348..8178)
                     /gene="efeU"
                     /locus_tag="ACHOS5_000786"
     CDS             complement(7348..8178)
                     /gene="efeU"
                     /locus_tag="ACHOS5_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP9775664.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            complement(8398..9906)
                     /gene="putP"
                     /locus_tag="ACHOS5_000787"
     CDS             complement(8398..9906)
                     /gene="putP"
                     /locus_tag="ACHOS5_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP9775665.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            10328..14290
                     /gene="putA"
                     /locus_tag="ACHOS5_000788"
     CDS             10328..14290
                     /gene="putA"
                     /locus_tag="ACHOS5_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9775666.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDERVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDGEAKTNIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAQVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQMLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(14330..14968)
                     /gene="rutR"
                     /locus_tag="ACHOS5_000789"
     CDS             complement(14330..14968)
                     /gene="rutR"
                     /locus_tag="ACHOS5_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP9775667.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            15256..16347
                     /gene="rutA"
                     /locus_tag="ACHOS5_000790"
     CDS             15256..16347
                     /gene="rutA"
                     /locus_tag="ACHOS5_000790"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP9775668.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMLCRAHLPALTQEVA"
     gene            16347..17039
                     /gene="rutB"
                     /locus_tag="ACHOS5_000791"
     CDS             16347..17039
                     /gene="rutB"
                     /locus_tag="ACHOS5_000791"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP9775669.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            17051..17437
                     /gene="rutC"
                     /locus_tag="ACHOS5_000792"
     CDS             17051..17437
                     /gene="rutC"
                     /locus_tag="ACHOS5_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP9775670.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            17445..18245
                     /gene="rutD"
                     /locus_tag="ACHOS5_000793"
     CDS             17445..18245
                     /gene="rutD"
                     /locus_tag="ACHOS5_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP9775671.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            18255..18845
                     /gene="rutE"
                     /locus_tag="ACHOS5_000794"
     CDS             18255..18845
                     /gene="rutE"
                     /locus_tag="ACHOS5_000794"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP9775672.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            18856..19350
                     /gene="rutF"
                     /locus_tag="ACHOS5_000795"
     CDS             18856..19350
                     /gene="rutF"
                     /locus_tag="ACHOS5_000795"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP9775673.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQESLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            19371..20699
                     /gene="rutG"
                     /locus_tag="ACHOS5_000796"
     CDS             19371..20699
                     /gene="rutG"
                     /locus_tag="ACHOS5_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP9775674.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGHVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(20782..20955)
                     /gene="ymdF"
                     /locus_tag="ACHOS5_000797"
     CDS             complement(20782..20955)
                     /gene="ymdF"
                     /locus_tag="ACHOS5_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9775675.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            21328..21924
                     /gene="wrbA"
                     /locus_tag="ACHOS5_000798"
     CDS             21328..21924
                     /gene="wrbA"
                     /locus_tag="ACHOS5_000798"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9775676.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            21945..22172
                     /gene="yccJ"
                     /locus_tag="ACHOS5_000799"
     CDS             21945..22172
                     /gene="yccJ"
                     /locus_tag="ACHOS5_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP9775677.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(22210..23451)
                     /gene="agp"
                     /locus_tag="ACHOS5_000800"
     CDS             complement(22210..23451)
                     /gene="agp"
                     /locus_tag="ACHOS5_000800"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP9775678.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGISGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKLLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(23732..23833)
                     /locus_tag="ACHOS5_000801"
                     /pseudo
     CDS             complement(23732..23833)
                     /locus_tag="ACHOS5_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23986..24906
                     /gene="cbpA"
                     /locus_tag="ACHOS5_000802"
     CDS             23986..24906
                     /gene="cbpA"
                     /locus_tag="ACHOS5_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP9775679.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            24906..25211
                     /gene="cbpM"
                     /locus_tag="ACHOS5_000803"
     CDS             24906..25211
                     /gene="cbpM"
                     /locus_tag="ACHOS5_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP9775680.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(25466..26065)
                     /gene="torD"
                     /locus_tag="ACHOS5_000804"
     CDS             complement(25466..26065)
                     /gene="torD"
                     /locus_tag="ACHOS5_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP9775681.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQLAEWFSLL
                     KSEPPLTAEVNDLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
     gene            complement(26062..28608)
                     /gene="torA"
                     /locus_tag="ACHOS5_000805"
     CDS             complement(26062..28608)
                     /gene="torA"
                     /locus_tag="ACHOS5_000805"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP9775682.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGTSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPKDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(28608..29780)
                     /gene="torC"
                     /locus_tag="ACHOS5_000806"
     CDS             complement(28608..29780)
                     /gene="torC"
                     /locus_tag="ACHOS5_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP9775683.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            29910..30602
                     /gene="torR"
                     /locus_tag="ACHOS5_000807"
     CDS             29910..30602
                     /gene="torR"
                     /locus_tag="ACHOS5_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP9775684.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSCHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(30575..31603)
                     /gene="torT"
                     /locus_tag="ACHOS5_000808"
     CDS             complement(30575..31603)
                     /gene="torT"
                     /locus_tag="ACHOS5_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP9775685.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WSHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYYY
                     QHTSAAKK"
     gene            31686..34429
                     /gene="torS"
                     /locus_tag="ACHOS5_000809"
                     /pseudo
     CDS             31686..34429
                     /gene="torS"
                     /locus_tag="ACHOS5_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            34501..35574
                     /gene="yccM"
                     /locus_tag="ACHOS5_000810"
     CDS             34501..35574
                     /gene="yccM"
                     /locus_tag="ACHOS5_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9775686.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMSVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAIYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(35623..35796)
                     /gene="gnsA"
                     /locus_tag="ACHOS5_000811"
     CDS             complement(35623..35796)
                     /gene="gnsA"
                     /locus_tag="ACHOS5_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9775687.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(35786..36016)
                     /gene="ymcE"
                     /locus_tag="ACHOS5_000812"
     CDS             complement(35786..36016)
                     /gene="ymcE"
                     /locus_tag="ACHOS5_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP9775688.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(35991..36179)
                     /gene="ymcF"
                     /locus_tag="ACHOS5_000813"
     CDS             complement(35991..36179)
                     /gene="ymcF"
                     /locus_tag="ACHOS5_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9775689.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(36190..36402)
                     /gene="cspG"
                     /locus_tag="ACHOS5_000814"
     CDS             complement(36190..36402)
                     /gene="cspG"
                     /locus_tag="ACHOS5_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP9775690.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            36688..36900
                     /gene="cspH"
                     /locus_tag="ACHOS5_000815"
     CDS             36688..36900
                     /gene="cspH"
                     /locus_tag="ACHOS5_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP9775691.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            37342..37647
                     /locus_tag="ACHOS5_000816"
     CDS             37342..37647
                     /locus_tag="ACHOS5_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775692.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATAVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            37754..38398
                     /gene="gfcB"
                     /locus_tag="ACHOS5_000817"
     CDS             37754..38398
                     /gene="gfcB"
                     /locus_tag="ACHOS5_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP9775693.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWNEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            38395..39141
                     /gene="gfcC"
                     /locus_tag="ACHOS5_000818"
     CDS             38395..39141
                     /gene="gfcC"
                     /locus_tag="ACHOS5_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9775694.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGMVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            39141..41237
                     /gene="gfcD"
                     /locus_tag="ACHOS5_000819"
     CDS             39141..41237
                     /gene="gfcD"
                     /locus_tag="ACHOS5_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9775695.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAANWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     IWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            41283..42422
                     /gene="gfcE"
                     /locus_tag="ACHOS5_000820"
     CDS             41283..42422
                     /gene="gfcE"
                     /locus_tag="ACHOS5_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP9775696.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTITGVHDLTETSRWIQTWPN"
     gene            42410..42856
                     /gene="etp"
                     /locus_tag="ACHOS5_000821"
                     /gene_synonym="yccY"
     CDS             42410..42856
                     /gene="etp"
                     /locus_tag="ACHOS5_000821"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP9775697.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            42876..45056
                     /gene="etk"
                     /locus_tag="ACHOS5_000822"
     CDS             42876..45056
                     /gene="etk"
                     /locus_tag="ACHOS5_000822"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP9775698.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITHILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PQVRSELDQAEEKLNAYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLASVIAQSDQKVLFI
                     DADLRRGYSHNLFNVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRYVGTSLLVARFSLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(45171..46469)
                     /gene="appA"
                     /locus_tag="ACHOS5_000823"
     CDS             complement(45171..46469)
                     /gene="appA"
                     /locus_tag="ACHOS5_000823"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP9775699.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAVIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFAGHRQTAFRELERVLNFPQSNLCLNRKKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPSQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNARGMCSLAGFTQIVNEARIPACSL"
     gene            complement(46549..46641)
                     /gene="cbdX"
                     /locus_tag="ACHOS5_000824"
     CDS             complement(46549..46641)
                     /gene="cbdX"
                     /locus_tag="ACHOS5_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP9775700.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(46654..47790)
                     /gene="appB"
                     /locus_tag="ACHOS5_000825"
     CDS             complement(46654..47790)
                     /gene="appB"
                     /locus_tag="ACHOS5_000825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9775701.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(47802..49346)
                     /gene="appC"
                     /locus_tag="ACHOS5_000826"
     CDS             complement(47802..49346)
                     /gene="appC"
                     /locus_tag="ACHOS5_000826"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP9775702.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(49480..50337)
                     /gene="hyaF"
                     /locus_tag="ACHOS5_000827"
     CDS             complement(49480..50337)
                     /gene="hyaF"
                     /locus_tag="ACHOS5_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP9775703.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            complement(50334..50732)
                     /gene="hyaE"
                     /locus_tag="ACHOS5_000828"
     CDS             complement(50334..50732)
                     /gene="hyaE"
                     /locus_tag="ACHOS5_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP9775704.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(50729..51316)
                     /gene="hyaD"
                     /locus_tag="ACHOS5_000829"
     CDS             complement(50729..51316)
                     /gene="hyaD"
                     /locus_tag="ACHOS5_000829"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP9775705.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAYIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVP
                     QPTEELRCLNYDCLSMENYEGVRLRQYRMRLEKQG"
     gene            complement(51313..52020)
                     /gene="hyaC"
                     /locus_tag="ACHOS5_000830"
     CDS             complement(51313..52020)
                     /gene="hyaC"
                     /locus_tag="ACHOS5_000830"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP9775706.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIMPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(52039..53832)
                     /gene="hyaB"
                     /locus_tag="ACHOS5_000831"
     CDS             complement(52039..53832)
                     /gene="hyaB"
                     /locus_tag="ACHOS5_000831"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP9775707.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(53829..54947)
                     /gene="hyaA"
                     /locus_tag="ACHOS5_000832"
     CDS             complement(53829..54947)
                     /gene="hyaA"
                     /locus_tag="ACHOS5_000832"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP9775708.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            55373..55460
                     /locus_tag="ACHOS5_000833"
     tRNA            55373..55460
                     /locus_tag="ACHOS5_000833"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55407..55409,aa:Ser,seq:tga)
     gene            55600..55929
                     /locus_tag="ACHOS5_000834"
     CDS             55600..55929
                     /locus_tag="ACHOS5_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9775709.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
     gene            complement(56088..>56472)
                     /locus_tag="ACHOS5_000835"
     CDS             complement(56088..>56472)
                     /locus_tag="ACHOS5_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP9775710.1"
                     /translation="LLTAEKARGMKGDEINAWRNAMEAANYMFEHNGRKWDYGKSTQT
                     RLEPSVAAAKAGKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQR
                     TMKKELEKLTSADEILAYRVGWAQE"
BASE COUNT        13730 a        14978 c        14233 g        13531 t
ORIGIN      
        1 ttattaaatg acatcaatta ttttatgact tatcagggcg agtccattaa ccttatttca
       61 ctggatcacg aatacccaga gtcctgtctt caaaatgaca caaacacata tttattagga
      121 agagtaatat ttctgtataa ctcaatgatt tataagttca taaactgtca ggaacatgaa
      181 accaataaca ttcactcagc tatgataaat aacctattac aggaagttga tatagccctt
      241 ggtaaaataa ataacattat agacagcaga aacatatcag ccccccatga actggcaaat
      301 attcttaccc gcgaaaaaat acttacaaca cgggaaaaaa aaagaaacct gataagcctg
      361 tttgatggat tcactttatt ccattgtgtt ggaatgataa cctttcttat ccattatctc
      421 agaacacctg aagaaaaagt tgaaaatata tttatgttat atggtgcaga taaaaacaat
      481 aaactacgca gaagactgat ttatgacgca ctaggaataa ttcagtctca gcaggagtga
      541 aacgttaaaa caggcaaatt gttttttata aacaggagat tgttgagtaa tctcctgttt
      601 ataaaaatac gctttcctga cgatctgata attatcagct caatacattt gacataaaag
      661 cggttcctta attactactg ccacgatcac atatcaaatc atgatcgtac aatatacatt
      721 ttcactatta tttattcaca atttgagaca tcacataatc cgcccatgcc tgcatcatcg
      781 gtacacgctg ctccagtaga tcagtacgat gatatgccgc ctcaacctta ttttttagcg
      841 tatgagcgag cgccctttcc gccagatccc gcgaataccc ctgttcacta caccagtccc
      901 tgaatgttga gcgaaaacca tgtgctgtgg caactcgccc cggaatgtca ctgacggctt
      961 tctttttacg cagaaaactt gtcaacacca tatcggaaag gatctgctgc tttctgggtg
     1021 aagggaacac cagttcatca tgcaggccac gtatattttc cagaatgtaa atagcctgcc
     1081 gggataaagg aacacgatgc tgtagcctag ctttcattct ttctgcaggt atagtccata
     1141 cccgcttatg aaaatcaatt tcagcccagc gcattcccct agcttcgccc gagcgagttg
     1201 ctgtaagtat caccattaat aacagtgcgc gggtaacatt ataaggttca tcactataca
     1261 cactggtcgc cacaaaaagc ggtaactgcc tccagggcat tgcgggttgg tgttcatcac
     1321 gtcctcttgt ctgctgagga agcaaatggt caaccacatc aacaggattt gccacacaaa
     1381 aaccgtgcgc ccatccccac tgcataacaa catgaatgcg ctgtttaacc cggcttgccg
     1441 tttctgacaa ggttaaccag actggacgca gtgtttctgc cacgtccgca gccgtaatcg
     1501 aatccagcgt ttttgctccc agttgaggaa acgcgtaatt ctcaagcgtc gataaccact
     1561 gccttacatg ctttggattt tcccatccag gagacagttc tgcatgtaca cgcctggctg
     1621 catcggcaaa tgttgggata acgactttct cagattcagc ctttttaatc tccagaggat
     1681 catcacctgc agcaagttgc tctcgcatta tccgggcagt acgtgcagct tcagcaatac
     1741 tgacctctgg gtaagttccc aatccagcat tacgtctttt ttgtgtcacc ggacttacat
     1801 aacgaaaaac ccatttcccc cgccccttta ctgaagaagg atgaagggtc agtccggtaa
     1861 ttcccccatg gggcaatggt ttgtcatcag gtttgatatg tcttgctttc gtatccgtca
     1921 atactgccat acgctaattc ccgtcactct ggtatgccat ccagtatgtc attagtccct
     1981 cgcttcacta agaatacatt agataatatc ggacaacaaa aagtgtaata cattgtttat
     2041 aatgaaatta tataacacac ctggattaag tcagatttat ttcaggcggt ccccctcacc
     2101 gccatattta aagaagagct cgtacgaaag tgcgagcttt ttttcgtcta ttgcatacac
     2161 cggggggatg agaagccccg accggggata cctggttaat gcaactgttc actctttgtc
     2221 gccgcgacta atgggcgaat tttgtcacgg gtatcccaga ccggagggat ttcctccaat
     2281 ttagtgacag aagctcgtac aaaaggcacc cgagcaagag tcataatcgt atccaaccaa
     2341 tggtcacgaa taatgcgatc tgccctttca tgactcggat catccagctc aataacaccg
     2401 actattttat tgttcttctt tgaaaccagc acataatcca ttgccatgcc attataaata
     2461 gcaaagaaac gctttctttc ttcttcgcta aactcaggtt gttctggaaa gttgatcaag
     2521 cgccccagag agacctggca atggatatgg caataatgtc caaaccattt ctccagatta
     2581 tcgaaataga ttttctcaca acgcgtaatt aaccactctt ttccttccaa taaaatgcct
     2641 tcggcaacca ttccctgaaa taactcatct cgttcttgcc aacgagaatc acgataggtt
     2701 ttctgcggtg tatagctcct tgtcgacgcc ttagcagcag ggggagttgc agatgaaggg
     2761 actggttgcg atttagagat gatcaccgat gaggcaactt ttcggagtgt caattttctg
     2821 gcaacaacaa aaccggcgac aaatgcaatt gtcaaacaag ttacaaaagc cataaagctc
     2881 ataattaatt ttcacctatc catgttgatt atatttcatt accaacgtta ctcgctgtag
     2941 cgtgttacat gccaacaatt caacgatcaa tatatagtta gtatcatgtt tttattatta
     3001 caaccacccc tattttgcca ttgcggcttg ccttccacaa aaaataacaa cacaaaacat
     3061 tagccaaaga attaattaag attaacctga catttttaat gttatcccta aaaaatataa
     3121 tcaggggaat gtattcggtg ttaatcagtc agtcaaatta tggtccatta ttgatggaac
     3181 ctcatacgca aaattggcac cagacctcct gtacaggttg aatggctgaa aaaagatagg
     3241 cagatgctcg ctttttgggc ttctgaaaga aaaactctcc gcactggcgg tattacactg
     3301 gtctggtgct atgacgacaa atgcccggta agagtatcta aacctgaatt catttcagct
     3361 tatcaacacg ctcaaagtcg ccccctgaac gttaacctga agtgaaaatc tttgctgtta
     3421 atgaaataaa acaaagcccg gttcgcccgg gctctgcacc gataatacac ttaactgtat
     3481 gcatgtagcg tacgttggca aagtgccgaa cgtacgcaat cctctttacc gaatcggacg
     3541 atcccgacca tttcatcctc ttcgaaacgt tccagcgcgt cacttaatcc ggagcgcacg
     3601 ccgcgaggca aatcgcattg cgtgatatca ccgttgacga taaccgtcac gttctccccg
     3661 aggcgggtta aaaacatttt catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg
     3721 acgactgcat tttcaaaggt acgtccacgc atataggcga acggcgcaat ttccaccttc
     3781 cctatttccg gtcgcaggca gtactgcata aaggaagccc ctaagcgacg gaccagcacg
     3841 tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga tatcaccagg taagaaacca
     3901 agatcttcat cggcttgcag aactggacgg gtaacgataa tcctgtcgac atccttatgt
     3961 atcagggcct ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc
     4021 gtggcaaata tcagctgctt actctcaata gccttcagat agtgcaattg cgcttcattt
     4081 cgcgcgagga tgggcgaggt atcgcgactg tcgcgggcca taccaatggc ttctacgccg
     4141 ctcatctgca caagcgaggt gaccgattct tcttcacgct gcttatggct gcgcgaatcc
     4201 cgtctcagca cacgttttgc ctcgcgacga gctttgatca ctgctttttg tcttcccatg
     4261 gagagcacct tgagttgttt gtattcatca cacgcgccgt tggcagcgcg attatgcgca
     4321 cgaacatcag agggttggct tccttgtaag ccatagtttg cttttggata aaatgccgaa
     4381 aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg aaaagaaagg atgaggttga
     4441 aaatacaaag tgacgacatg actaccggag gagaaatctc cgcgagtggt ggcgcgctga
     4501 ttatctaaaa catgtccagt acaggacgtt accatccgcg atctccatag tgactgacta
     4561 tcactgccgg gaacttccgc tgctacttaa taagtacaac agatttcgca ttcattgcaa
     4621 caatatattc acttatattt aactataaaa caccatttca gtgacattag tttctactgg
     4681 aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt gctttcagca ggattcgcag
     4741 tactggtaaa agcttgccgg atgcggcgtg aacgccttat ccggcctacc gacccgcacc
     4801 gattgtaggc atgataagac gcgcaagcgt cgcatcagtg tacgaacgcc tggtgcagcg
     4861 taaacgcctt atccgcctaa aaacattaaa cccgcaataa tgcgcttccg agataatcgt
     4921 tcgcgtcctt cacacccggc agcgcaaaga aatagccgcc accgataggt ttgacatatt
     4981 cctccagcgc ctcgccattc aacctttttt gtactgtcag gaagcccttt tccagatcgt
     5041 gttggtagca gacaaacagt agccccatat cgagttgccc ggaattggtg acgcccaacg
     5101 aataactgta gccacgacgc agcatcaggc tggactcgct ctccaccgtg cggggattcg
     5161 ccagccggat atggctgtcc agcgcaatca ccttcccttc cggatcgcta gcgtaatcgg
     5221 gaacatcatg ctcgtgctgc atgcccagcg gcgcaccggt ttgcttatca cgaccaaata
     5281 tcgtctgctg ctctttcagc ggagttctgt cccaaaattc cactcgaaac tgaatcaagc
     5341 gcactgcctg atagctgcca ccgattgtcc acgcaggctc ctgctgatct gccgtcaccc
     5401 acaccacttt ttgcatcaac ttcgcattct ggctatcggg attggcggtg ccgtctttaa
     5461 agcccagcaa atttatcggc gtctctttgc ctttactacg cgctgcgtgg tcggaaataa
     5521 acccttcccg cttccagcgc acgctgagca aatccggcgt gtgtttgatg atatcgcgca
     5581 gcgcatggat caccgtgtcc tgggtgttgg cgcaaatttg tagcaacaca tcaccatgac
     5641 ataacgccgc atccagcgag tcgttgggga aacgcgtcat cttctgcaac tttttcggca
     5701 tctgtggcgc aaggccaaag cgctcatcaa acaatgagtg acccaccgat aacgtgatgg
     5761 tgagattatc gggcgcaatg tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg
     5821 gatttggcgt ttctggtgcc gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca
     5881 agcgaaacaa ccgttcaaga tcggctttat cgctggcaag cacatcaaac gccaccagca
     5941 tcattgcagc ctgttgtggt gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt
     6001 tctcattgcg tgcatccggt gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag
     6061 cgaccggaca acttcccgcc agcgccagcg caccaatccc tttcagtaaa cgtcggcgtg
     6121 acggttcatt cacgccgttt tcatcttcat actgcataac gcttagtcca gccccagcac
     6181 accgcgaagt tgcgccagat cttccgccag cgcagtaatc ggtcctttca gtgcattccg
     6241 gtcagcatcg gtcagtttgt cgtaattttc aaaaccgtct ttagtacggt atttcgccag
     6301 aatggtatcg acctttttga agttggcatc gacttttgcc aacagttccg gattcgcttt
     6361 ttgtagttgt ggacgcagca gatcgacaat tttctgcgag ccttcaacgt tagcctggaa
     6421 atcccacaga tcggtgtggc tgtagcgatc ttcttcaccg ctgattttgc tggctgccac
     6481 ttcctcaatc agtccggctg caccgccgac cacttttgaa ggagggaaag ccagttcact
     6541 gatgcgtttt tgcaaatcga ccacatcggt ataaagctgg tcagcatact tatccatccc
     6601 tttggtggtg ttatcgccaa acaatgcttt ttccagacgg tggaaaccgg tgaatttcgg
     6661 atcggcggct ttttgctcgt aatcatcttc acgggcgtca atgctgccat ccagatcgga
     6721 gaacagttca gcaatcggtt cgatgcgctc atagtgctgg cgcgtcggcg catacagtgc
     6781 tttcgctttt tcgatatcgc ctgctttaat agcgtcagta aaggctttgg tgtcggtcac
     6841 cagttgcgtg gtttccgcca tgacatacgc tttatatgca gtaattgcac caccaagact
     6901 taacagcgca tcactttgcg ccgcatccgc cgttgcttca cctttgacga tcaacttccc
     6961 tttcgggtta gtcagcagac cgcaggtcat atcgtattcg ccaggctgta aattcgccgt
     7021 cattttctgg ctaaaaccag gggcgatatt ttcccgctct tccaccacca tcacgccttt
     7081 gaggatctcc cactccagcg ccttctggct gtggttctga ataatgaact gtgttttccc
     7141 ggcgttaacc gtaatggtca tcggctcgca ctgcttatcc gtcacggtca ctttgacttg
     7201 cggaatatcg gcggcgttag ccataaaagc agaagagaac agcgcagcca cgctcaactg
     7261 caatgcgtta cggcggaagt taatggtcat gacgagacta tccctttaaa gtatgttgta
     7321 actaagcaag agtttgcgtc gtatttgcta cacggagcgg gacgcagtcg cccctgcgcg
     7381 tggtggcaga acaaatgcca ccagcgccgg gatgagataa ataaaccaga cggcgacttc
     7441 gctgacgctc ggtgcttcct gatagccaaa aatcccttcc atcagtgtgc caaacagcga
     7501 gtgagttgag agcaccgcgc tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc
     7561 ggcttcatga aatgcgcgaa tggcaccagc tgccagccct gcggcgacga agagaataaa
     7621 cagactggtc catttaaaaa atgcaccaag attgaggcga ataccgcccc agtagagcag
     7681 gaagccaagc accacggctg tagcaagacc aagcattgca cccagcggcg gccagatccc
     7741 gacatcttgt tgaaatgccg ccagcaggaa aaagaccgac tccagccctt cccttgcaac
     7801 ggcaaaaaag accatcatca ccagcgccca gccatgatga tttccacgct gcaatgcgct
     7861 atcgaccgcc tgctccagtt gtactttgac gttgcgcgac actttgcgca tccagaaaac
     7921 catccaggta aggatcacta cggcgatcac cgccacaatg ccttcaaaca gttcctgttc
     7981 tttttgcgga aattcgccgg tggtttcgtt aatgaagata cccaagccca ggcacaacgc
     8041 agcggcaagc aacacgccaa tccacattac accaatccat cggcctcgct gggtacgctt
     8101 aagatagctg gcaatcaaac tgacaatcag cgcggcttca agtccttcgc gcaacataat
     8161 gagaaacgga acaaacatgc cacccaccct taaacgtttt tctgagtcaa tctatgcaaa
     8221 gaaaagtaaa tcgcagtgat agcgattatc attacgatga agaaaaatac aagtggaatg
     8281 tagtgataat gatgagaaga tgtaaaaata ttttgagaaa aaacgcagag ggcaatcact
     8341 gattgccctc tgcctgcttc ctgttatgac gtgaaatttc cggtaaaacg ccttccctta
     8401 cccttcctgc aaccgtgacg gcggagccga atgatagtgc gcatcggcct cggcaaagcg
     8461 tttttgcatc gccgctgacg gcgctttacc cagcaaacta aacactacaa tcccgatact
     8521 gccgaagata aagcccggaa taatttcgta cagacccagc cagccgaact gtttccagac
     8581 gataaccgtc agcgcaccga tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga
     8641 ccacatcacc gagaacagca ccaccggacc aaacgctgcg ccaaagcctg cccacgcgta
     8701 gctcactaag cccagcacgc ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc
     8761 caccaccagc accatcactc gccctaccca caccagctct ttctggctgg cctgtttacg
     8821 cagaaacgct ttgtataaat cttcggtaat cgcactggaa cataccagca gctggcaact
     8881 taacgttgac attaccgccg ccagaatcgc cgacagcaaa atcccggcaa tccacgggtt
     8941 aaacagaatt tgcgccagtt caataaacac gcgttcagcg ttctggttta ccgcacctgc
     9001 taccgccggg tgctcgttaa agtaagcgat accaaagaag ccgacagcca ccgcccctgc
     9061 caggcagagg atcatccagg tcatactaat acgacgcgca tggacaatgc tgtggtgaga
     9121 atctgccgcc ataaaacgcg ccaggatgtg cggctgcccg aagtaaccca gcccccagcc
     9181 catcagcgag ataatggcga caaaattcaa ccctttgagc atatccacgt tttcaatgct
     9241 cttttgtttg attacttcca gcgagtcacc aaagccaccg acactgataa tgacgataac
     9301 tggcgtcagg atcagggcaa aaatcatcag actggcctgt acagtgtcag tccagctcac
     9361 cgcgaggaaa ccgccaataa aggtgtaaag gatcgtcgcc gcagcccccg cccacagagc
     9421 cgtttcgtag ctcatgccaa aggtactttc aaacagacgc gcgcctgcca caatgcctga
     9481 agcgcaataa atggtgaaga acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg
     9541 gcttttatct tcaaagcgcc cggtgaaata atccggcaat gttaaggcgt tattgttgta
     9601 ttcggtatgc acacgcaacc gcccggccac cagcttccag ttgatccacg cgcctaatgt
     9661 cagaccaatg gcgatccagc tttcggaaat cccggaaaga aaaacagcgc ctggcaaccc
     9721 cattaacagc cagccgctca tatccgacgc acccgccgat aatgccgtca cgaatggccc
     9781 aagactacga ccgcccagaa tatagtcgtc gaagtttttc gttgatcgcc aggcgataaa
     9841 cccaatcaat atcatgccaa agatatagac acaaaatgtc accaacatcg gtgtgctaat
     9901 agccatctaa agtctccaaa aaattattat cggcaatgtc gaaacttgcc gttatatctg
     9961 ccaccggaac ggggtaacag agtttatgtt ttaccagggc gaccgtatcc tgccggaagc
    10021 gctggttatt cacaatcgat ttaacacacc atttacatta aattttagtg ctcagcgaca
    10081 ctatttttca tcaggttgca ctctctcaca ttttttgcgg ttgcaccttt caaaaatgtt
    10141 aactgccgca gagaaaaagt ctgagttatt ttttaatccc tgtcatatcg atttcgttta
    10201 ttaacatttc attcattttt aagcttgcta cgcaggtcac atttaacacg gttgcacaaa
    10261 gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag
    10321 taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat
    10381 taagtctgcc gcgacacgta tcgatcgcac accacactgg ttaattaaac aggcgatttt
    10441 ttcttatctt gaacaactgg aaaacagcga tactctcccg gagctaccag cgctgctttc
    10501 tggcgcggcc aatgagagcg atgaagcacc gactccggca gaggaaccac accagccttt
    10561 cctcgacttt gccgagcaaa tattgcccca gtcggtttcc cgcgccgcca tcaccgccgc
    10621 ctatcgccgc ccggaaaccg aagcggtttc tatgctgctg gaacaagctc gcctgccgca
    10681 gccagtagct gaacaggcgc acaaactggc gtatcaactg gccgataaac tgcgtaatca
    10741 aaaaaacgcc agtggtcgcg caggtatggt tcaggggtta ttgcaggagt tttcgttgtc
    10801 atcgcaggaa ggcgtggcgc tgatgtgtct ggcggaagcg ttgttgcgta ttcccgacaa
    10861 agccacccgt gacgcgttaa ttcgcgacaa aatcagtaac ggtaactggc agtcacacat
    10921 tggtcgtagc ccgtccctgt ttgttaatgc cgctacctgg gggctgctgt ttaccggcaa
    10981 actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa
    11041 gagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca
    11101 gttcgtcacg ggcgaaacca tcgcggaagc gttagccaat gcccgcaaac tggaagagaa
    11161 aggtttccgt tactcttacg atatgctggg cgaagccgca ctgactgccg cagatgcaca
    11221 agcgtatatg gtttcctatc agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg
    11281 tggcatctat gaaggtccgg gcatttcaat caagctgtcg gcgctacatc cgcgttatag
    11341 ccgcgcccag tatgaccggg taatggaaga gctttacccg cgtctgaaat cactcaccct
    11401 gctggcgcgt cagtacgata ttggtattaa catcgatgcc gaagaggccg atcgtctgga
    11461 gatctccctc gatctgctgg aaaaactctg tttcgagcct gaactggcag gctggaacgg
    11521 catcggtttt gttattcagg cttatcaaaa acgctgcccg ttggtgatcg attacctgat
    11581 tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg
    11641 ggatagtgaa atcaagcgtg cacagatgga cggccttgaa ggttatccgg tttatacccg
    11701 caaggtgtat accgacgttt cttatcttgc ctgtgcgaaa aaactgctgg cggtgccaaa
    11761 tctaatctac ccgcagttcg cgacgcacaa cgcccatacg ctggcggcaa tttatcaact
    11821 ggcgggtcaa aactactacc cgggtcagta cgagttccag tgcctgcatg gtatgggcga
    11881 gccactgtat gagcaggtca ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg
    11941 tatttatgct ccggtcggta cacatgaaac gctgctggca tatctggtgc gccgcctgct
    12001 ggaaaacggt gctaacacct cgtttgttaa ccgtattgcc gacacctctt tgccactgga
    12061 tgaactggtc gccgatccag tcactgctgt agaaaaactg gcgcaacagg aagggcaaac
    12121 tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa
    12181 ctcggcaggg ctggatctcg ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct
    12241 caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaatcgg tcgcggctgg
    12301 tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct atgtgcgtga
    12361 agccacgccg cgtgaagtag aacaggcgct ggaaagtgcg gttaataacg cgccaatctg
    12421 gtttgccact cctccggctg aacgcgcggc gattttgcat cgcgctgccg tgctaatgga
    12481 aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa
    12541 cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactatgccg gacaggtgcg
    12601 ggatgatttc gctaacgaaa cccaccgtcc attagggcct gtggtatgta tcagtccgtg
    12661 gaacttcccg ctggctattt tcaccgggca gatcgctgcc gcactggcgg caggtaacag
    12721 cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt
    12781 gctggaagcg ggtgtaccgc caggcgtggt gcaattgctg ccaggtcggg gtgaaaccgt
    12841 gggcgcgcaa ctgacgggtg atgaacgcgt gcgcggggtg atgtttaccg gttcaaccga
    12901 agtcgctacg ttactgcaac gcaatatcgc cagccgcctg gacgctcagg gtcgcccaat
    12961 tccgctcatt gcggaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac
    13021 cgaacaagtc gtaatagatg tgctggcctc ggcgttcgac agtgcaggtc agcgttgttc
    13081 ggcactgcgc gtgctgtgcc tgcaagatga aattgccgac cacacgttaa aaatgctgcg
    13141 cggcgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc
    13201 ggtgattgat ggcgaagcga agaccaatat tgagcgccat attcagacta tgcgtagcaa
    13261 aggccgcccg gtgttccagg cagtgcggga aaacagtgaa gatgcccgtg aatggcaaag
    13321 cggcaccttt gtcgctccga cgctgatcga actggatgac tttgccgaat tacaaaaaga
    13381 ggtctttggt ccggtgctgc atgtggtgcg ttacaaccgt aaccagctcc cagagctgat
    13441 cgagcagatt aacgcttccg gctatggtct gacgcttggc gttcatacgc gtattgatga
    13501 aaccattgcc caggtcactg gctcggccca ggtcggtaat ctgtacgtta accgtaatat
    13561 ggtgggcgcg gtggttgggg tgcaaccgtt cggcggcgaa gggttgtccg gtaccgggcc
    13621 gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct
    13681 ggcggtgacg ctcgcgcgtc aggatgcgga gtatccggtc gatgcgcagt tgaaagccgc
    13741 attgactcag ccgctaaatg cactgcggga atgggcggca aaccgtccag aattgcaggc
    13801 gttatgtacg caatatggcg agttggcgca ggcaggaaca caacgattgt tgccagggcc
    13861 gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt gtattgcgga
    13921 tgatgaacag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggta gccagatgct
    13981 gtggccggat gatgcgctgc atcgtcagtt agtgaaggca ttgccatcgg cagtcagcga
    14041 acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg cggtgatctt
    14101 ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgccgcgc gggatggtgc
    14161 aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg aacggctgta
    14221 tatcgagcgt tcgctgagtg tgaataccgc tgctgctggc ggtaacgcca gtttaatgac
    14281 gatcggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc
    14341 gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aacacctcat
    14401 cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc aaaatcggcg taatgttgag
    14461 tggaagccca aatcataaaa atcaaatgct gcgggtcaat cggcgcgagt ttgccgcttt
    14521 taacccaacc ggcaatgagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca
    14581 gttcatccat taacagcggt gcacctgcca gcatctccat acagaacagg cgcgaagcct
    14641 gcggataatc gcgggaaact tccagcttca gacggatgta ttctttgatc gccgccagcg
    14701 gggcgaaatc ttctcgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca
    14761 gtacggctat atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa
    14821 cacccgccaa ctccgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag
    14881 tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgcttac
    14941 ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga
    15001 tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacga atgcatgttt
    15061 tatgtgcaac tgttttgacc gtttagtcca ctttttttca ggcattttag acaacttaac
    15121 attaacacat taataacatt cactttttaa aaactggcat ccgctttgca aacaagccat
    15181 tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac
    15241 gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc
    15301 atttcgaccc acgcgccgca gtacatgccg acatttgaac tgaataaagc cattgtgcaa
    15361 aaagcggagc actaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc
    15421 ggcaaaactg agttctggga tcataacctt gagtcgttca ccttgatggc ggggctggca
    15481 gccgtcacct cacgcattca gatatacgcg acagctgcca ccttaacgtt acctccggca
    15541 atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac
    15601 ctcgtgactg gctggcagaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac
    15661 tatttctccc gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg
    15721 ggctcgggga aaagcgattt taaaggcgat tttttcacta tggatgattg tcgcgtcagt
    15781 ccgcaaccga gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg
    15841 ttctccgccc ggtatgccga ttttaacttc tgtttcggca aaggcgtaaa tacacccacg
    15901 gctttcgccc cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg acgcgacgtt
    15961 ggctcttatg tgttgtttat ggtgattgcc gacgaaaccg acgatgccgc tcgtgcaaaa
    16021 tgggaacact acaaagcggg cgcggatgaa gaggcgttaa gctggctaac tgaacaaagt
    16081 cagaaagata cccgctcagg tactgacacc aacgtccgtc agatggccga tcccacttcg
    16141 gcggtaaaca tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta
    16201 gatgaagtcg caaacgtgcc tggtgccgaa ggcgtgctgt tgaccttcga tgattttctg
    16261 tcgggaatcg aaaccttcgg cgagcgcatt caaccactga tgctgtgccg cgcccatctc
    16321 cctgcgctga ctcaggaggt ggcatgatga caaccttaac cgcacgaccg gaagccatta
    16381 ccttcgatcc gcagcaaact gcgctgatcg tggtggatat gcaaaatgcc tatgccacgc
    16441 caggcggcta cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca
    16501 acattcaaac cgccgtgacc gcagcgcgaa cggcagggat gctgatcatc tggtttcaaa
    16561 atggctggga tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat
    16621 cgaacgccct gaaaaccatg cgtaatcagc cgcagttgca ggggaaattg ctggcgaaag
    16681 gctcctggga ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc
    16741 cgaagccgcg ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgtg
    16801 gaatacgcca tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac
    16861 gcgacggctt ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg
    16921 ggccggaatt tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca
    16981 gcgacgtcga aacgttctgc gacgcgcttt cttccacgtc ctttgctcgt atcgcttaag
    17041 gagtttaacg atgccaaaat ccgtaattat tcctgctggc agcagcgcac cgctggcccc
    17101 cttcgttccc ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga
    17161 tcaacataac aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga
    17221 aaccatccgc aaggtcatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag
    17281 catctttatt accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt
    17341 cccgggtgac aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct
    17401 ggtggaaatc gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct
    17461 cacctccccc ttatgctgat gcgcccgtag tggtgttgat ttcgggtctt ggcggtagcg
    17521 gcagttactg gttaccgcaa ctggcggtgc tggttcagga gtatcaggta gtctgttacg
    17581 accagcgcgg caccggcaat aatcccgaca cgctggcaga agattacagt atcgcccaga
    17641 tggcagcgga actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg
    17701 gccatgcgct cggtgcgctg gtgggaatgc agctggcgct ggattatccc gcgtcggtaa
    17761 ctgtgctggt cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc
    17821 aggttcgcga acagttacta catagcggcg gcgcgcaggc atgggtggaa gcgcagccgt
    17881 tgttcctcta tcccgccgac tggatggcgg cccgcgcacc tcgccttgag gcagaagacg
    17941 cgctggcact ggcgcatttt cagggcaaaa ataatttact gcgtcgactt aacgccctca
    18001 aacgcgctga ctttagtcgc catgcggatc gtatccgctg cccggtgcaa atcatctgcg
    18061 ccagtgatga tctgctggtg ccatcagcat gttccagtga acttcatgcc gctctgcccg
    18121 atagccagaa aatggtgatg cgctatggcg gacacgcctg caacgtgacc gatcccgaaa
    18181 cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc
    18241 tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg
    18301 atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacgggaga
    18361 tttatgccct gatgaaatgg gggccaacat ctgctaactg ttctccggca cggatcgtgt
    18421 ttatccgcac tgcagaagga aaagaacgtc ttcgcccggc gctttccagc ggcaatctgc
    18481 aaaaaaccct gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac
    18541 ggttaccaca gctgtttcct cacggcgatg cccgcagttg gtttacctcc agtccacaac
    18601 ttgccgaaga aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgcct
    18661 gccgtgcgct ggggctggat accggcccga tgtcgggctt tgaccgtcaa tacgttgacg
    18721 acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg
    18781 attccagcaa actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt
    18841 tgtaaggagc aaacaatgaa cattgtcgat caacaaacct ttcgcgatgc gatgtcctgt
    18901 atgggcgcgg cggtcaatat catcactacg gacggtccag ccgggcgcgc cggattcacc
    18961 gccagcgccg tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt
    19021 ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacacttagc
    19081 gccgggcagg agtcgctttc aaaccttttt ggcggcaaaa cgcccatgga acaccgcttt
    19141 gccgccgccc gctggcagac tggtgtgacc ggatgcccgc aactggaaga ggcactggtt
    19201 tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat tttgttttgc
    19261 gccatcgaag cgattcatcg tcacgccaca ccctacgggc tggtgtggtt cgatcgcagt
    19321 tatcacgcgc tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt
    19381 tcggttttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg
    19441 atgaacgatt gccgtttgct caaacggcag taatgggcgt tcaacacgcg gtggcgatgt
    19501 ttggcgcgac ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa
    19561 tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcacgtg cccagttatc
    19621 tcggttccag cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aatggtcagg
    19681 gaattaatcc gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata
    19741 ccgtcattgg cctggtggtg atgaaaatcg gtacgcgctg gattgaacga ctaatgccac
    19801 cggttgtgac aggtgcggta gtgatggcga ttggcctgaa cttagcgcct atcgcagtga
    19861 aaaatgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg
    19921 ggctggtagc ggtgtttacc cgtggcatga tccagcgatt attgattctc gtaggattaa
    19981 ttgtcgcctg tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg
    20041 attttactct cgtcagccat gctgcctggt tcggtctgcc acatttttcc actcctgctt
    20101 ttaatggtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg gcggaaaact
    20161 taggtcatct caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc
    20221 gggcattcgt aggcgatggg ctggcgacga tgctttctgg ctctgtcggc ggtagcggcg
    20281 taaccaccta cgccgagaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg
    20341 tgtttgttgc cgctgccgtc atcgccatgc tgttgggctt ttcgccgaaa tttggcgcgc
    20401 tgatccatac tattccggct gcggtgattg gtggtgcatc aattgttgtg ttcgggctga
    20461 ttgccgtcgc aggcgcacgg atctgggtgc aaaaccgtgt ggatttaagc cagaacggta
    20521 atttgattat ggtcgcagta accttagtgc tgggtgcggg tgattttgct ctcacgctgg
    20581 gcggttttac gttgggaggg atcggtacag ccacctttgg cgcgatttta ctcaatgcgt
    20641 tgttaagcag aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa
    20701 cagaaaaccg ccggggtaac cggcggtgat ggcgttttga cgagctacag acaaaaatgc
    20761 atgcgtcatt gattagcccg gctagttgtc gcttttaccg tgactgctct tgccaccttt
    20821 tttacctgct tcagatgcgc gctgcgggtc atttttgaaa ttacccccgc tgtgctgtcc
    20881 acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc
    20941 tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc atagaattaa
    21001 cgcggcgtat ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc
    21061 gggcagaccc gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct
    21121 ccaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt
    21181 cattttgcga caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga
    21241 aatttgcact ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa
    21301 tagagataac acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg
    21361 tacggacata ttgaaacgat ggcacgcgca gttgctgagg gtgcaagcaa agtggatggc
    21421 gcagaagttg tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca
    21481 ggcggtaaaa cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc
    21541 attatttttg gtacacctac ccgctttggt aacatgtccg gtcaaatgcg taccttcctc
    21601 gaccagacgg gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt
    21661 agttccaccg gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt
    21721 gcgcatcacg gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt
    21781 tcacaggttc gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggttca
    21841 cgccagccca gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt
    21901 ctggcagtta aacttaacgg ctaatcttca acaggaggat acgcatgcca actcaagaag
    21961 cgaaagctca tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg
    22021 aggccatttt cgaagtcgcc gggtacgacg aaaaaatggc ggaaaagatt tgggaagaag
    22081 gtagcgatga agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg
    22141 gcgaacagac catcgaacgt aaaaacgttt aaccagcgac tcccccgctt cacgcggggg
    22201 agttttctgt tatttcaccg cttcattcaa tacgctatca aacttatcca ttgggcaaaa
    22261 accgttggca tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg
    22321 cagggttaac gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt
    22381 catcaggtcg cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat
    22441 cggcgtgcgt tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag
    22501 agaggcaatg ttggagtcgt gcccaactaa taccgtgatt ttcggtgcac tggtgcgatc
    22561 ggtgaccagg gctttgtcga tataactgac cagcagtttc gcaacattgc gcgccacttc
    22621 cggtgaggta aacaggctgt cctggtaacc gtttttcagc ttcgacaaca ccttccactg
    22681 ctggtcagat ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg
    22741 caaagtaaac gcatctacca gcgagttgcc gactttcagc ggcccggaaa tgcccggttc
    22801 ttgttgatac ttcgcgctaa aggtattttt gccatctaca agcgaacatt gttgcttctc
    22861 tttacacgca ggagaatctt tatagttaac gatttgttcc agtagcttgt agctgtcggt
    22921 aagctggagt ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc
    22981 ggaatcatcg gtgatcactg ggttaaaggt ggggtccata gtgcccattt tttcctggtg
    23041 atgaacagga atatcacacc cagggaatgc gccagtaata aagaactgcg cggtcgcgac
    23101 ggtgcgttgc agactattgg cataggcata aacggtgtcc ggtggcgggc attcccctga
    23161 tttcaccatc ccctgttctg ccagccattc acgcatataa tggcccatat acacttcgag
    23221 cacgccccct ttagtggtga gctgcccacc ggggacgtcc cattcaggcc atttattcgg
    23281 cgtcgactgc tccagcacac tgccattgtt cgccagcggc gcacgtaagt tatggcggct
    23341 catcatgagc acttgctgta gctgatagcc ttccggtacg gtttgtgcct gagcatttga
    23401 agcgagtaaa actatccctg ccacagttgc ggcgattagc gttttgttca ttcctcacac
    23461 ctcttttgtt atcaataatt aaagagtgtg acagaaatat gtcatttttc cgggaacagg
    23521 ttcgcaaaat ggctcgttga acgcctgttt ttctatattc ccctttaaaa acaggcaagt
    23581 tttgacgcaa actatgcctg attagccccg gcctcgttta cgccgctaat gattacatca
    23641 gtagctataa agaaaatcag cggcgttacc attaccaata aaaaaagaat gtgagactaa
    23701 tagaaatggt ttttagcaca tgtctttatc attaaattgc ataacgattt aaatagtcga
    23761 gttgtgcatt cctttcctta ttgtttaact tgtaagcatg agccaaaagt tcaggagtga
    23821 ggtaaataaa atatgttcct cccaccttga ggcgacctcc cctccgcgat gatttcaata
    23881 acatattctg tgttggcata tgaaattttg aggattaccc tacacttata ggagttacct
    23941 tacaggggtt ccttcaattt gtgttgattt acgcgagata acgctatgga attaaaggat
    24001 tattacgcca tcatgggcgt gaaaccgacg gacgatctca agacaatcaa gaccgcctat
    24061 cgtcgactgg cccgcaaata ccatcctgat gtcagcaaag aaccggatgc cgaagcccgc
    24121 ttcaaagagg tcgctgaagc ctgggaagtg ttaagtgatg aacaacgtcg cgctgagtat
    24181 gatcagatgt ggcaacatcg caacgatccg caatttaacc gtcagttcca tcatggcgac
    24241 ggtcagagtt ttaacgccga agattttgac gatatcttct cgtcaatttt cggtcagcat
    24301 gcccgccaga gccgtcaacg ccccgccaca cgcggccacg atattgaaat cgaagtggcg
    24361 gtattcctcg aagaaacgct tactgagcat aagcgtacca tcagctataa cctgccggtt
    24421 tataacgcct ttggcatgat cgaacaggaa atcccgaaaa cgctgaatgt gaagatcccg
    24481 gcgggcgtcg gcaatggtca acgcatccgc ctgaaaggcc aggggacgcc gggcgaaaac
    24541 ggcggtccaa atggcgacct gtggctggtg attcacattg caccacatcc gctgtttgat
    24601 attgtcggcc aggatctgga aatagtggtg ccggttagcc cgtgggaagc ggcgctgggt
    24661 actaaagtca ccgttccaac actgaaagaa agcattttgc tgactatccc gcctggcagc
    24721 caggccgggc aacgattgcg cgttaaaggc aaaggtctgg tgagcaaaaa acagaccggc
    24781 gatctgtatg cggtactgaa aatcgtgatg ccgccgaaac cggatgaaaa cactgccgcg
    24841 ctgtggcagc aactggcaga cgcccagtcg tcttttgatc cacgtaaaga ttgggggaaa
    24901 gcataatggc taatgttacg gtgactttta ctattaccga attttgcctg cataccggca
    24961 tctctgaaga ggagttgaat gaaattgtcg gtttgggggt ggttgaaccg cgtgagatcc
    25021 aggagacaac ctgggtattt gacgaccatg ccgccattgt ggtgcaacgc gcggtacgcc
    25081 tgcgtcatga actggctctg gactggccgg ggatcgcggt ggcgctgacg ttaatggatg
    25141 atattgcgca cctgaagcag gaaaaccgcc tgctgcgcca gcggctttcc cggtttgtgg
    25201 ctcacccgtg agtttgttgc ctgatgcgac gcttaacgcg tcttatcagg cctacaaaga
    25261 gcactgttcc cgtaggccgg ataaggcgtt catgccgcat ccggcaactg cgccataatg
    25321 cctgatgcga cgcttaacgc atcttatcag gcccacaaaa agcactgttc ccgtaggccg
    25381 gataaggcgt tctcgccgca tccggcaact gcgcactaat gcctgatgcg acgcctgccg
    25441 cgtcttatca ggcctacaaa cggcattatc ttttttggta gtcacactcc actaacacca
    25501 gcaataactg gcttagtgcc gcgtaaaaac caaagctgtc atactgtcga caacgcgcag
    25561 caaactctgg taaccattcc cgcagcgccg tcagtgtttt ttgccgcaaa ccgtcgattc
    25621 ttcgcgcagg aacggtcccc tctcccagcg aaaaatgcag atgactcaac aactcgagat
    25681 agatcgccag atgatctgcc ggttcgttga aattgccgct ggtttccatc cctgcctcaa
    25741 ctaacaagcg tttaatctct tgctcgtcct gtttgtaggc cgatgcatac ggcagcgccg
    25801 cctgcttgtc agtcatcaga aacaggccgc aaaagtcagc ggccagttcc agacgggcat
    25861 cgtcacgtac tgtcagcgtg gcaatactgt tttccagatc attcacctcc gctgtgagcg
    25921 gcggttcgct tttcagcaac gaaaaccatt cagccaactg cgcactggcg atttgcgtca
    25981 gttgttcatc gtccagttca cgggagaaca actgcgccag ccaggcgtaa acacaagcaa
    26041 tctgttgtgc tgtcagcgtg gtcatgattt cacctgcgac gcgggaacat attcgcactg
    26101 cgccaccatc tccacggggc cgttaaacgc cgtcacctgc tccactgttc cgttgtactt
    26161 ctcaatttcc accagcgtag tgtgcgcact ggtcgcctgc gccagctgcg aagtaccgat
    26221 gtcgatggtc aacacgttgg ggttaccgta tttgcacagc gcccctggct cgccgccttt
    26281 gtccggatcg taccatgccc cttcgtgaat tcgcgccacg ccgggtgcat aacggtcaga
    26341 aaccactgct ccagccaaca cctgaccacg aacgttaaag acgcgtacca catcaccgtg
    26401 acgaataccg cgcgcgctgg catccttcgg gctaataaat actggctctt tacccgctac
    26461 ggtatattgc tgacgcagag tttccgactc acataactgc gagtgaagtc ggaaatccgg
    26521 atgcacagat tgcagatgca acggatactt ctgcgagcca ggcccaccgt gggagcgttc
    26581 gattttctca aaccacatcg gatgcccctg acaatcgtcg tagttcatgt cggcgatggt
    26641 ttttgagtag atctcaatca ggccactcgg cgtgcccagc ggttcgagat ccggatcttc
    26701 gcggaatgcc tggtggcgaa caaacatctg cggatggtca aactcgacgt actctttgtt
    26761 attccagaag tcatcaaacg ctggcagatg aacgccgcgt cctttgcctt gttgtacacc
    26821 ttcctgccag atgcgtttca gccagcccat ctcgtccagc ccttcggtaa aggcttcttc
    26881 gcgattaaag cggcggcaca gctcgcggaa aatatcaaag tcgttgcgcg cctcgaactg
    26941 cggcggcacg acctgtttca tggcgatgat gccacgattg gagtggttgc cgtactggtc
    27001 gagatcgtta cgctcaaact gcgtggtcgc aggcagcacg atatcggcaa agcggcaggt
    27061 tgaggtccac tggttatcta tggcgataac cgtttccagc ttgcgccagc cttcaataat
    27121 gcggttgatc tgctgatggc gatggaacgg gttagttccg gcaaaaatac acattttcag
    27181 cggcggcagt tttaccgatt taccgttcca gttgatcact ttccccggtt cgaggatcgc
    27241 atcgataaaa cgggcaatcg gaatggtgct gctgtagcct ttgtagtcac tgttgtcgtg
    27301 aacaggcgga atcgacgtag agccggagaa accactcaaa ataacgcctt tacgccccgg
    27361 cgtgcctgcg ccgttatagt gccagccaaa accaaaacca ccacctggca ggccaatttg
    27421 ccccagcatc gccgccagaa cgacaatcat ccacgcccac tgttcaccgt gctgcatacg
    27481 ctgtacgcac cagccagcaa taatctgcgt tctgttcgcc gccatctgcc gtgccagccc
    27541 acgaatggtt tcggcatcaa tgccggtcag tttttctgcc catgcggcat ctttcggctg
    27601 accgtctttc tcacccagca gatacggcag gaactgctca aaacctacac agtagttagc
    27661 gaggaagttt ttgtcgtaca ggttttcact gtacagcgta tacgccagcg ccagttgcag
    27721 cggcacgtca gtttgcgggt taaccgcaat gtgcttcaca tgctcgcgcc ccagatactc
    27781 atgggtggat gtaacaaccg gatcgatgct gatgacctca atttcaccgg cggcgacttt
    27841 cgctttcaac tgctcgtaat attcataaac atcgtgatcc gggcaccacc agttcgcttg
    27901 ctggtttttc agcaaatcgg agccccacag cacaatggtt ttgctgttct gcaataccag
    27961 cggccaggag gtttgctgtt catacacttc cattgagcca accacgcgcg gcaggatcac
    28021 ctgcgcagca ccggtagagt aatctccgcc cgtaccaacg ctattaccat gcaaggcaat
    28081 agctttcgcc agcatccccg aagcgttatg gaacatcccc gtcgattgcc aaccactggc
    28141 ggtcagcaag gcactcggcc cgtgagtttt ctgtacgcgt tccagttctt catagaacat
    28201 gtcgagggct tcatcccagc tcacgcgcac aaaacggtta tcaccgcgct gggaggtgtc
    28261 gctcagatgg cgcttacgca gccagtccac gcgtaccatc gggtaacgaa tacgcgccgc
    28321 gttgtgtaca tgatccggca atccggcgat cattttcgac ggatatttat ccagttcgaa
    28381 cggttttgct gccacaaagc gaccatcctt caccgtcgcg cggatagccc cccagtgcga
    28441 cccggtaaga atgccctctt tcgagatgac agcctcagtc gccgcttgcg ccgcagtcgc
    28501 acggcgcggc gttaacaatg acgtccccag catcccggcg acggttaagc cgccgagttg
    28561 tgccagaaaa cgccgacgtg atgcctgaaa gagatcgtta ttgttcatta tttttcttcc
    28621 ttcttatcgc cgtgagcctt acctgcggtg tcagacgcat tcatttgcag atatttcaac
    28681 aaggtgcgtt cttcacgttt atcgaggctg gtaaagccaa tcatgccgtt gagcgtgccg
    28741 atccaaccgt tagcgtcaaa gtgggagatt tccggtgcgc catggcactg gttacaggtg
    28801 ccgttgtaca gagaatccgc ataagcccag atcggtttga tatcgttcac catgtcgcct
    28861 ttcttcatcc acgcagtagc ctgcaacttg ctccactcgg tattggtgtc ggcaacggtg
    28921 gttttctcca gtgttttcac ctgctgctgc acatcaccac gaatcgaggc aacaaagatg
    28981 cgtttacctg ggaattgggt gagtacacgc tgacgtcctg cactttccgt ccaaccagtg
    29041 atttcaattt gcagccagtc gccgtcacgt ttaaggactt tcacttccga agcaggcagc
    29101 agagaaccag acgcttcttt atcgcctttc gccgcataaa tcggcttaat atcaatggag
    29161 tacagcgtgt caccactatc attagcactg gcgcgcagct catcgaactg cttacggaag
    29221 ccactactca tatcaggtaa ctggtgggca atacctttat gacagtcgat gcaagattga
    29281 ttatctttcg ctgccacctt catctgacgc gccgcttcag gatgctgctt cgcatgatcc
    29341 atcgcatcgt agttatggca ggagcggcag gttgccgagt tgttttcttt cattcgcgcc
    29401 cattcacgct cggcaagttc cgcgcgtttg gcttcaaatt tctcaggtgt atcaatggag
    29461 tgggcaataa aggtctggta aatgtcgttg ctcgcttcca gtttgcgctt caccatgcct
    29521 ggaatatccg gcgggatatg acagtcatgg cattcagctc gcacgccgga ggcgttctgg
    29581 aaatgcaccg actgtttata ttcttcatac accggttgca tactgtggca actgacacaa
    29641 aattcggttg tgctggtgac tttgatccca acgtgtggca atacaatcag cgcaatgcca
    29701 atcacaatcc cagtagcgac cagcgccagt accgaccaac gagcactggg tcggcgtagc
    29761 gcgttccaga gtttccgcat aatagcccct gtaaaattat ggtttagtga agcgatctta
    29821 atgagcaaat atgaacagcg gcactggtca ggatgaacgg cttacggcag aatatgaaca
    29881 gatatgaaca gaatgagtaa aaccctctga tgccacatca cattgttatt gttgaagatg
    29941 agccggttac ccaggcgcga ttacagtctt acttcactca ggaggggtat accgtttccg
    30001 ttacggcgag cggtgccggg ctgcgggaaa ttatgcagaa tcagccggtg gatttgattc
    30061 tgctggatat caatttgccc gatgaaaatg gcctgatgtt aacccgcgcc ctgcgagaac
    30121 gctcaacggt ggggattatt ctggttaccg gacgcagcga tcggattgac cgtattgttg
    30181 ggctggaaat gggcgcagac gattacgtca ccaaaccgct ggagctgcgc gaactggtgg
    30241 tacgagtgaa aaatttgctc tggcgaatcg acctcgcgcg acaggcgcaa ccgcacactc
    30301 aggacaactg ctatcgcttt gccggttatt gcctgaatgt gtcgtgccat acgctggagc
    30361 gggatggaga gccgattaaa ctgacccgcg cagagtatga aatgctggtg gcgtttgtga
    30421 ccaatcccgg cgaaattctc agccgtgaac gtctgctgcg tatgctctct gcgcgtcggg
    30481 tggaaaaccc tgacctgcgc accgtcgatg tgttaattcg tcgtttacgc cataaactca
    30541 gcgcggattt actggtgaca caacatggcg aaggttattt cttagccgct gatgtgtgct
    30601 gataataata gaccggacga aatccccccg gtgacagcga gcggcggata tgttcgcggt
    30661 cggcattttt cggcgtcaga actaaaatcg gtgggctgac attatcagac accgattgcc
    30721 cctgtaattg cctgatggcc tgctcaaccg ccagttcccc ctgccagacc atttgatcgc
    30781 tggcagccat aatcactctt ccccgcttca gcccgcgata cacctgatgt gaaagataaa
    30841 acgacaccac ggtaagcggt gttttcaggt tacgcccttc tcccattgcc gcctctgccg
    30901 caatggccgt tccggcaacg acgtcgattt ctggatggcg ctccagcatc tcctgcaaca
    30961 ggttacgctg gatttcaata tcgttatcgc caagcgcaat atcaacaata cgtaccgggc
    31021 ttccggcaat ggctgcgcga aaaccctcga ccatctcctt actgcccccg gcgttatcgg
    31081 gtccgggcat caacagcacg ttcagtggtt taccgtggct ccattgcacc agatatcgtc
    31141 ctggttgata gcccatctga aaccagggca caccaacgcg gcttttcacg tggggagcat
    31201 caatagcatt caccagttcg atcaccggca gacttgctac ctgcttttgc agatcgggaa
    31261 atgacgtcgt gctactaccg agcaaaatgg cctctgcgcc ccactgttta cactggtcga
    31321 tttgtgcttg ctgcgtagcc aactggctgt agccgcctgc ctccagcact tttaaatcca
    31381 caccgtagcg gcgagcagcc tcctgcatac catagttcaa cgataaccag tatgaatctt
    31441 tcaggctggg ataaagcgcg cacagtttcc atgcgcgttt ggctttaagc ggcgtagagg
    31501 cttgcaccgt gtaatgctgc gcatcatgcc agcgcaacag gttatcagcc gaaaatgccg
    31561 ataacatgaa aagggaaaga agtaaaaata gcagtacgcg catgatagcc tcatcaataa
    31621 taaggcttta tgctagatgc attctgcttt gcgactcaac ctttttcacc taaagtgcac
    31681 cgaccgtgaa tttaaccctg acccgaagac tctggatggg ctttgccctg atggcgctgt
    31741 taaccctgac cagtaccctg gtgggatggt acaacctgcg ctttatcagc caggtggaaa
    31801 aagacaacac tcaggcattg attcctacca tgaatatggc gcgccagttg agcgaagcca
    31861 gcgcctggga acttttcgcc gcgcagaacc tgaccagtgc cgataacgaa aagatgtggc
    31921 aggcgcaggg gcgaatgctc acagcacaaa gcctgaagat taatgcgttg ctgcaagcgt
    31981 tacgggaaca aggttttgac accaccgcta ttgaacaaca ggagcaggag atctcccgtt
    32041 cgttacgtca gcaaggggaa ctggtgggac agcgtttgca actgcgccag caacaacagc
    32101 aactcagtca gcagatagtc gccgccgccg atgagatcgc acgcctggcg caaggtcagg
    32161 cgaataatgc ggcaacctcc gccggagcga cccaggccgg gatttacgat ttgatcgaac
    32221 aacatcagcg tcaggccgca gaaagtgcac tcgatcggct gattgatatc gatcttgagt
    32281 atgttaacca gatgaatgaa ctgcgcctta gcgccctgcg ggtgcagcaa atggtgatga
    32341 atctggggct ggaacagatc cagaaaaatg cgccaacgct ggaaaagcag ctcaataatg
    32401 cggtgaaaat tctgcaacgt cggcaaatac gcattgaaga tccgggtgtt cgtactcagg
    32461 tagcaacaac gttaactacc gttagccaat atagcgattt gctggcgctg tatcagcagg
    32521 acagtgaaat cagcaatcac ctgcaaactc tcgcccaaaa taacatcgcc caattcgcgc
    32581 agtttagtag cgaagtcagt cagctggtcg acaccattga gctgcgtaat cagcacgggc
    32641 tggcacatct ggaaaaagcc agtgcacgcg ggcaatacag cctgttattg ctgggattag
    32701 tttctctttg cgcactgatt ctgatcctct ggcgcgtggt ttatcgctca gtcacgcgcc
    32761 ctcttgcaga acaaacgcag gcgctgcaac gcttgctgga tggtgataac gactcccctt
    32821 tcccggaaac cgctggcgta cgggaactgg ataccatcgg gcggctgatg gatgcgtttc
    32881 gcagcagtgt tcatgcactg aatcgccacc gcgaacagct ggcggcgcag gtcaaagcgc
    32941 gtacagctga attgcaggaa ctggtgatag aacaccgaca ggcacgggcg gaagcagaaa
    33001 aagccagcca ggcaaaatcg gcgtttctgg cggcgatgag ccatgagatc cgcacaccgc
    33061 tgtacggtat tctcggcacc gcacagctgc tggcagataa ccccgcactt aacgcccagc
    33121 gcgatgattt gcgggcgatt accgactctg gtgaatcgtt gctgaccatc ctcaacgata
    33181 ttctcgatta ttcggctatc gaagcaggcg gcaagaatgt ttcggtcagc gatgaaccct
    33241 ttgaaccgcg cccgctgctg gaaagtaccc tgcaattaat gagcggaagg gtgaaaggtc
    33301 gcccgattcg cctggcaaca gaaattgccg acgatgtacc gactgcgtta atgggcgatc
    33361 cgcggcgtat tcgtcaggtc atcaccaacc tgttgagcaa cgccctgcgt ttcaccgacg
    33421 aagggcacat tatcctgcgt agtcgcactg atggcgaaca atggctggtc gaagtggaag
    33481 acagcggctg cggtattgat cccgcgaaac tggcagaaat cttccagcca tttgttcagg
    33541 ttagcggcaa acgcggcggc accgggctag ggctgactat cagtagccgt ctggcccagg
    33601 cgatgggcgg cgaactgagc gccaccagca cgccggaggt tggaagctgt ttttgtttac
    33661 gcttgccgtt acgtgttgcc actgcacccg tgccaaaaac agtcaatcag gcggtgcgtc
    33721 tggacggttt acgtttgctg ttaattgaag ataacccgct aacccagcga attaccgttg
    33781 agatgctgaa catcagtggt gcgcaggttg ttgctgttgg caatgccgtg caggctttag
    33841 aaacactgca aaatagtgaa ccgtttgctg ccgcactggt ggattttgat ctaccggata
    33901 tcgacggcat tacgcttgcc cgacaactgg cacagcaata tccgtcgctg gttttgattg
    33961 gctttagcgc ccatgtcatt gacgaaacac tgcgacagcg taccagttcg ctatttcgcg
    34021 ggattatccc taaaccggtg ccgcgtgaag tgctcggtca attactggcg cactatctcc
    34081 aactgcaagt caataacgat caaccgctgg atgtatcgca actcaatgaa gatgctcatt
    34141 taatgggggc ggagaagatc cacgaatggc tgatattatt taaacaacat gccctgccgc
    34201 ttctcgatga aatcgacatt gcccgcgcca gtcaggataa cgagaaaata aagcgtgccg
    34261 cacatcagct aaaaagcagt tgctcgagtc tgggaatgcg tagcgccagc cagcaatgcg
    34321 cacaactgga gcagcagcca ttatctgccc cccttccaca cgaagaaatt acacgcagtg
    34381 ctgccgctct ggaagcatgg ttaataagaa agacctgaac gcgatttgaa tcaatatcag
    34441 ccagttaaga acaactagaa tagtggcatt tggtatgcaa cttaacggac tggcaaaatc
    34501 atggcagaga aaaaaagaac ccgctggcag cggcggccag gcacgacggg cggcaaatta
    34561 ccgtggaatg actggcgcaa tgccacgacc tggcgtaaag cgacgcaatt attactgttg
    34621 gcaattaata tttatattgc catcacgttc tggtattggg tgcgctatta cgaaacggca
    34681 ggtagtacga catttgtcgc cagaccggga ggtatcgaag gctggctacc gattgccggt
    34741 ctgatgaatc tgaaatatag ccttgcaaca ggtcagttac cgtccgtcca cgccgccgcg
    34801 atgctgttat tggtcgcttt tatcgtcatc agtctattac tcaaaaaggc cttttgttca
    34861 tggttatgcc cggttggtac gctttcggaa ttaatcggcg atctcggtaa caaactgttt
    34921 ggtcggcaat ttgtccttcc ccgctggctg gatattcctc tgcgcggcgt gaagtatttg
    34981 ctgttgagtt tttttctcta tatcgcgtta ttgatgcccg ctcaggcgat tcactatttt
    35041 atgttgtcgc cctacagcgt ggtgatggac gttaaaatgc tcgatttctt tcgtcatatg
    35101 gggaccgcga cattaatcag catgatcgtt ttgctgattg ccagcctgtt tattcgccat
    35161 gcctggtgtc gttatctttg cccatatggc gcgctgatga gcgtggtttc gctattatcg
    35221 ccgtttaaga ttcgtcgcaa tgccgaaagt tgtatcgatt gtggcaaatg cgcaaaaagt
    35281 tgcccatcac ggatccctgt cgataaatta attcaggtac gaacagtgga atgtaccggc
    35341 tgtatgactt gcgtagagtc atgtccggta gcctcaacat tgaccttttc actgcaaaaa
    35401 cctgcggcaa ataaaaaagc ctttgcgttg tctggatggt taatgacgct actggttatg
    35461 gggattatgt ttgcggcgat tggttacgca atctatgcgg gagtatggca aagcccggtg
    35521 ccggaggaat tgtaccaacg cttaattccg caatcgccaa tgattggtca ctaaattagc
    35581 aacaagcgga acatcacctc gcacggggtg atgttcacaa aatcacatta ttttgatttt
    35641 gacatcataa ctttcaagcc cggtcatttt ttctcgtgcg gtgaagcgaa tttcagagac
    35701 ttctttccct gtattcttgt tcagctcggc gattttttgc gcgataaagt cggcgatttc
    35761 cgtttcggct tgttttttta actcttcaat attcactgtt aacctcttct ggtcttaaaa
    35821 gacagcaaca cgccagtaag gtgttggttc ccggtaccct ggtcaggtaa gaaatgttat
    35881 aagccatctc cccgaaagat tcaataatca acaggttatc gcttactata catacagcat
    35941 tgaagaaaaa taaggcagat ataaaaaagg ctccacgagg gagcctgata ttattttgtg
    36001 aaatccagcg gcgcatggtc agtgcgtatt tgcagcgcga cgttatcaaa ggtgatcatc
    36061 gtcgatttac aaaaaatgca ctttgcaccc aaagggtttc gctccgtcac atcgaatgcc
    36121 gatgtgcggt actgtgaacc atggcaacat ggacagcgga aatggagatg ttgagtaata
    36181 acggcaacct tagagcgtaa caacgttcgc tgccgcggga ccacgttgcc cctgctcaat
    36241 agaaaattca actttctgat tttcgttcag cgtgcggaat tcattgctct ggatggcagt
    36301 gaaatggacg aaaacgtctt tgctgccatc atcaggagtg ataaagccaa aacctttatc
    36361 tgcgttaaac cattttacta aaccagtcat tttattagac atattttact tccttcattt
    36421 tgggcctttc ggcaaacagg gctttaatac agaaactact tagtgttcag gagagagact
    36481 caaaagaagg ggataatgcc tgataatgag aactgcttta gtaaactact ttgtatgctg
    36541 tctgtctttc aaaccgacgc agctattaac gcatgataat tgagattaag caaattttat
    36601 tttagccgtc cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca
    36661 gcgcagtatt ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc
    36721 tttgatcgca aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagtccag
    36781 gtccatattt ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgcgtg
    36841 gaattttgcc gcgtaaatgg cctgcgtgga ccaacagcgg caaatgttta tctctcttaa
    36901 tatgacgctg cttgtttaaa actgactggt tttattggaa tagatcaata gtcaacacca
    36961 cacattataa cgtaagttac tttttcatca agaaactaat ccatttaacc gaatatctct
    37021 gtacctaata gttgtaaacg ccacttaaca gccagttata gtaccatttg aaactatatt
    37081 accaacaata gttaatagct ttctcaacaa ttttcttatc tttaatttgt atttttctaa
    37141 gatttatctt atgctcacaa gtcgtatttc cagagggaaa tcgttaacaa tcttatattt
    37201 ccctctgcgc gcctttccta cccgcagtat atagtgcaag cggtatgagg aaagggagat
    37261 ttcacaagga tgttatactc aaagtagata atcctgatct caagcgtacg tattgtccat
    37321 gcgatgaata aaggaaaagt tatgaaacac aagctttctg caatccttat ggccttcatg
    37381 ttgacgacgc cagctgcatt tgctgcccct gaagcagcaa acgggaccga ggcaacgacg
    37441 ggtacaactg gcaccacaac gaccactacc ggtgcaacca cgactgcagc taccactggt
    37501 ggcgtggctg ctggcgctgt cggtacggcg acggctgtcg gtgtggcaac ggctgttggt
    37561 gtggcgacgt tggcagttgt tgcagcaaat gacagtggcg atggcggttc tcataacacc
    37621 tcaaccacca cgtccactac gcgctaatac cactttaacg cgtcgaaatt aaagttaaat
    37681 gatacccggt tgcgatattc gtaccgggct ttagagattt gcattttttt aattaacctt
    37741 tcaaggatta aaggtgcgcc ctcttatttt atcgattttc gcactatttc ttgcgggatg
    37801 tacgcacagc cagcaaagta tggtcgatac atttcgcgcc agcctattcg ataatcagga
    37861 tatcaccgta gcggatcagc agatccaggc gttgccttat tccacgatgt atttacgcct
    37921 taatgaaggg caacgaatct ttgtggtact gggatatata gaacaagaac aaagcaaatg
    37981 gttatcccag gataacgcca tgctggttac ccacaatgga cgtcttttaa aaaccgtcaa
    38041 acttaataat aatctgctgg aagtgacaaa ttccgggcag gaccctctgc gtaacgcgct
    38101 ggcaataaaa gacggcagcc gctggacgcg cgatattctc tggaacgaag acaaccattt
    38161 tcgctctgcg accctgagtt ccactttttc ctttgctgga ttagagacgc tgaatattgc
    38221 gggtcgcgat gtgctgtgta atgtctggca ggaagaggtg acttccacgc tgccagaaaa
    38281 acagtggcaa aacacattct gggtcgattc ggctactggc caggttcgtc aaagtcggca
    38341 aatgttaggc gcaggggtta ttcccgtaga aatgacgttt cttaaacccg caccatgaat
    38401 aaattacagt cgtatttcat tgccagcgtc ctttacgtaa tgacacccca tgcctttgcg
    38461 caaggaatgg tgactattta tctgcctggc gaacaacaga cactttccgt tggccccgta
    38521 gagaatgttg cccagttggt gacacaaccg caactgcgcg atcgcctctg gtggcctggt
    38581 gccttgctga ccgactccgc agcgaaagct aaagcgctta aagactacca acatgtcatg
    38641 gcacagcttg cttcctggga agcagaagct gacgacgatg tggcggcaac gattaaatcc
    38701 gtgcgccagc agttactcaa tctcaatatc actggtcgct taccggtcaa actggaccct
    38761 gattttgtgc gagtggatga gaacagcaat cccccgctgg tcggggacta tacgctctat
    38821 accgtacagc gtccggtgac tattactctg ctgggtgcgg tttctggggc tgggcaattg
    38881 ccctggctgg caggccgtag cgttaccgat tatctgcaag atcacacccg tcttgcaggc
    38941 gcggacaaga ataacgttat ggtgattacg cctgagggtg agactgtcgt cgcccctgtt
    39001 gccctgtgga ataaaagaca tgtggaacca ccgcctggca gccagctttg gttaggcttt
    39061 tctgcgcatg tactgcccga aaaatatgct gatttaaatg accagattgt gtctgtttta
    39121 acgcaacgtg ttcctgactg atgaagaaga actcttatct tttaagctgc ctggccattg
    39181 ccgtctccag tgcctgtcat gctgaagtat taacctaccc ggatccgctg ggttcgtcgc
    39241 aatcagactt tggcggcaca ggattgttgc agatgccaaa tgcgcgcatc gcaccggaag
    39301 gtgaattcag cgtcaactac cgggataacg atcagtaccg tttctactcc acgtccgtgg
    39361 cgctgttccc atggctggaa ggcaccattc gttatacgga tgtgcgcaca cgcaaatata
    39421 gccagtggga agatttcagc ggcgatcagt catacaaaga caaatcattc gattttaaac
    39481 ttcgcctgtg ggaagaaggt tactggctac cgcaagtggc gtttggtaaa cgtgatattg
    39541 ctggtacggg tctgttcgac ggtgaatatc tggtcgccag caaacaagcc ggaccgttcg
    39601 atttcaccct cgggatggcc tggggctacg ccggtaatgc gggcaatatt accaacccgt
    39661 tttgtcgggt aagcgataaa tattgccatc gcgcagagtc tcacgacgcg ggagatatca
    39721 gctttagcga tatctttcgt ggtccagcct ctatttttgg cggtattgag tatcaaacgc
    39781 cgtggaatcc tctgcgtctg aaactcgaat acgacggcaa caattaccag aatgattttg
    39841 cgggcaaatt gccgcaggca agtcatttca atgttggtgc tgtgtatcgt gctgccaact
    39901 gggcagatct taaccttagc tacgagcgtg gtaacacgtt gatgtttggc ttcacgttac
    39961 gaaccaattt caacgatctg cgccctgccc tgcgcgatac gccaaaaccg gcatatcaac
    40021 ctgcgccgga atctgaagga ttgcagtaca ccacggtagc aaaccaactt accgcactga
    40081 agtacaacgc aggttttgaa gcaccggaaa ttcagctacg cgataagacg ctgtatatgt
    40141 ctggtcagca atacaaatac cgtgactctc gcgaagcggt cgatcgtgcc aaccggattc
    40201 tggtgaataa cctgccgcaa ggcgttgaga agattagcgt gacgcaaaag cgcgagcata
    40261 tggcgatggt gactaccgaa accgacgtag ccagcctgcg caaacagttg gccggtacag
    40321 cgcctggtca atcagagcca ctgcaacaac aacgtgttga agctgaagat ctttctgcct
    40381 ttggtcgggg ctatcgtatt cgtgaagatc gctttagcta ctctttcaac ccaacacttt
    40441 cacagtcgct gggcggcccg gaagatttct atatgttcca gttggggctg atgtccagcg
    40501 cccgctactg gtttaccgac cacctgctac ttgatggcgg tattttcacc aatatttaca
    40561 acaactacga caagtttaag tcttcgctgt tacccgcgga ctctaccctg ccccgcgtgc
    40621 gcacgcatat ccgtgattac gttcgcaatg acgtttatct caacaacttg caggcgaact
    40681 actttgccga cttaggcaat ggtttctatg gccaggtgta tggcggttat ctggaaacga
    40741 tgtatgccgg tgtcggttcc gagctgcttt atcgcccgct agatgccagc tgggcgctgg
    40801 gtgtggacgt taactacgtg aagcagcgtg actgggacaa catgatgcgc ttcaccgatt
    40861 attccacgcc aacaggtttc gtgacggctt actggaaccc gccgacgctc aatggcgtac
    40921 tgatgaaact cagcgtgggg caatatctgg caaaagataa aggcgcaacg attgacgttg
    40981 ccaaacgctt tgacagcggt gtggcagtag gaatatgggc ggcaatcagt aacgtatcaa
    41041 aagatgacta cggcgaaggc ggctttagta aagggtttta tatctcgatt ccgttcgact
    41101 tgatgaccat tgggcctaac cgcaaccgcg cggtggtttc gtggacacca ttgacgcgtg
    41161 atggtggaca aatgctgtca cgcaaatacc agctctatcc aatgacggca gagcgagaag
    41221 taccggttgg acaataacct gaagtaattc ataacattac aatgactgat aaatataaac
    41281 caatgaaaaa gaacattttt aagttttcgg tactgacatt ggctgttctg tctttgacag
    41341 catgtaccct tgttcctggt cagaatttat cgaccagtaa caaggatgtc attgaactac
    41401 ctgacaatca gtatgacctg gacaaaatgg tcaatatcta tcccgtcacg ccaggattga
    41461 ttgatcagct gcgcgccaaa ccgattatgt cgcaggctaa tccggagctg gagcagcaaa
    41521 tcgcgaacta tgaatatcgc atcggcattg gtgatgtgtt gatggttacg gtctgggatc
    41581 acccggaact aaccaccccc gcagggcaat accgtagcgc cagtgatacc ggcaactggg
    41641 ttaacgccga tggtgcgatt ttctacccct atattggtcg cctgaaagtg gccgggaaaa
    41701 cacttaccca ggtacgtaac gagattacag cgcgtctgga ttcagtcatt gaaagcccgc
    41761 aggtcgatgt cagcgtcgcg gcgttccgat cgcaaaaagc gtacgtcacc ggcgaagtca
    41821 gcaaatcggg tcagcagcct attaccaata ttcctctgac catcatggat gcgattaacg
    41881 ccgcgggagg tctgaccgca gatgcagact ggcgcaacgt ggtgctcact cagaatggcg
    41941 taaagaccaa agtaaacctc tatgcgctga tgcagcgtgg cgacttgcgc cagaacaaac
    42001 tgctgcaccc gggcgatatt ttatttattc cgcgcaacga tgatctgaaa gtcttcgtga
    42061 tgggtgaagt gggtaaacag agcacgctga aaatggaccg cagcggtatg accctggcgg
    42121 aagcgctggg taacgctgag ggtatgaatc aggatgtcgc cgacgcgacg gggatttttg
    42181 ttattcgtgc aacgcaaaac aagcagaatg gcaaaatcgc caatatttat cagctgaatg
    42241 cgaaggacgc atcggcaatg atactcggaa cggaattcca gcttgaacct tacgacattg
    42301 tttatgtcac tactgcgccg ctggctcgtt ggaaccgtgt gatttctctg ctggtaccga
    42361 cgattaccgg ggtgcatgac ctgacagaga cttcacggtg gattcaaaca tggcccaact
    42421 aaaatttaac tcaatcctgg tggtttgtac cggcaacatt tgccgttcgc ccattggcga
    42481 acgcttgctg cgtaaacgtc tgccaggcgt aaaagtcaaa tcagcaggtg ttcatggtct
    42541 ggtaaaacac cctgcagatg cgacagcggc agatgttgct gctaatcatg gcgtatctct
    42601 tgaaggacat gccggacgta agctcactgc tgaaatggca cgaaattacg atctgattct
    42661 ggcaatggag tcagagcata ttgctcaggt taccgcaatc gctcccgagg ttcgcgggaa
    42721 gacaatgctg tttgggcaat ggctggaaca gaaagagatc ccggatccct atcgtaaaag
    42781 tcaggacgca tttgaacatg tctacggtat gttggagcgc gccagtcagg aatgggcgaa
    42841 gcgcctcagc cggtaatttg agtttataaa atacgatgac aactaaaaat atgaatacgc
    42901 caccaggcag cactcaggaa aatgagatcg atctgcttcg tctggtcggc gagttatggg
    42961 atcaccgtaa gtttattatc agcgtgaccg cgttattcac gctgatcgct gtcgcttact
    43021 cgctgttaag cacgccaatt tatcaggcag atactctggt ccaggttgag cagaaacagg
    43081 gcaacgccat tctcagcggc ctgagcgata tgatccctaa ctcatctccc gagtctgcac
    43141 cggagatcca actgctgcaa tcgcgcatga ttctcggtaa aaccattgct gaactgaatc
    43201 tgcgcgacat cgttgagcag aagtattttc cgattgtggg tcgcggctgg gcgagattaa
    43261 ccaaagagaa accaggtgag ctggcgatca gctggatgca tataccacaa ctgaatggtc
    43321 aggatcagca actgacgctg acggttgggg aaaacggcca ctatacgctg gaaggtgaag
    43381 agttcaccgt caatggtatg gtcggccagc gtctggaaaa agatggcgtt gcgctgacta
    43441 tcgcggacat taaggccaaa ccaggaacgc agtttgtcct gagccagcgt accgaactgg
    43501 aagcgattaa cgcgttgcag gaaaccttta ccgttagcga acgcagtaaa gagagcggga
    43561 tgctggaact caccatgacc ggtgacgatc cgcaactgat tactcatata ctgaacagca
    43621 tcgctaacaa ctatttgcag cagaatatcg cgcgtcaggc ggcacaggat tcgcaaagtc
    43681 ttgagttcct gcagcgccag ttaccgcagg tacgcagtga gttggatcag gctgaggaaa
    43741 aactgaacgc ctatcgtcaa caacgtgatt cggtagactt gaatctggaa gcaaaagcgg
    43801 ttcttgagca gattgttaat gttgataatc aactcaatga gctgactttc cgtgaggcag
    43861 agatctccca gctatataaa aaagatcacc cgacctatcg tgcgctgctg gaaaaacgcc
    43921 agacgctgga gcaagaacgc aaacgcctga ataagcgggt atcggcaatg ccttccactc
    43981 aacaggaagt gctacgttta agccgtgacg tagaagcggg ccgtgcggta tatctgcaat
    44041 tacttaaccg ccagcaggag ttgagtattt cgaaatccag tgccattggt aacgtgcgga
    44101 ttatcgaccc ggcagtcact cagccgcagc cagtgaaacc gaaaaaagcg ttgaatgtgg
    44161 tgcttggttt tattcttggc ctgtttattt cggtgggtgc cgtgctggcg cgtgcgatgt
    44221 tgcgtcgtgg tgtagaagca ccggaacaac tggaagagca cggcatcagc gtttatgcca
    44281 ctatcccgat gtccgagtgg ctggataaac gcacccgtct gcgtaagaaa aatttatttt
    44341 ctaatcagca gcgccatcgt actaaaaata tccccttcct ggcggtggat aacccggcgg
    44401 attctgctgt agaagccgta cgtgcactac gaaccagtct gcatttcgct atgatggaga
    44461 cggagaataa cattctgatg atcaccggtg cgacgccaga cagtggtaaa acgttcgtca
    44521 gttcaactct ggcgtcggtg atcgcccagt ccgatcaaaa agtgttattt attgacgccg
    44581 acttacgccg tggttattcg cataacctgt tcaacgtgag taatgaacat ggtttgtctg
    44641 aatatctggc aggtaaagat gagctcaaca aagtgatcca gcattttggc aaaggaggct
    44701 ttgatgtgat tactcgcggt caggtgccac ctaacccgtc tgaactgctg atgcgcgatc
    44761 ggatgcgtca attactggaa tgggcgaacg accattacga tctggtgatt gtcgatacgc
    44821 cgccgatgct ggcggtgagc gatgctgcgg tcgtgggtcg ctatgtcggc accagcctgc
    44881 tggttgcgcg ttttagcctg aacaccgcca aagaggtgag cttgtcaatg caacgtctgg
    44941 aacaggcagg cgtcaatatt aaaggcgcta tcctcaatgg tgtgattaaa cgcgccagca
    45001 ccgcttacag ttacggctat aactattacg gttatagtta ctccgagaaa gagtaaaact
    45061 gaaagtggcc ggatgcggcg tgaacgcctt ttccggccta cgcatcagac agttcttcgt
    45121 ttgtcatcaa cctcagagca ttcaggtaac tgaatgctct tttttatgca ttacaaactg
    45181 cacgctggta tgcgtgcttc attcacgatt tgcgtaaaac cggccaacga acacatgccc
    45241 cgcgcatttc gctcttcaca tcctgccagg gtcagtttca cctctccggg cggcgtattt
    45301 aatgacagcg gcgttttatc acgcatctgc tgtaaagtct ggaagaccag cgaaacctga
    45361 atccactggc tgttatcgct gagccgacgc cagcgttcaa acaccagttc accacctggc
    45421 ggcgtgttat ccggctgacc tggaagtgtc cagttgagct ccagtgcgcc gccgagattt
    45481 gccagattag tatcgtgtcc ggcaataaac agcactgaag tgggtaatgt cacaccatac
    45541 gcctgttttt gcgatggatg gggcgtcaac gctgccatga tcaaatccaa taacggggtg
    45601 gcgcgactgc gggcaacctc tggcgtgcgt tgtagtaaat aaaattgcgc gttatgtaga
    45661 cttagcaagg tgttccactg gtgtgaatcg gtgatccttc cccaccccgg ctccggcatt
    45721 ccctgtgctt gttgcaggag aaatatctcc gtcagcattg atgcgaggct taccgcaccg
    45781 gttaatgaga cattgtcggc gctcaccttg agttccgatg gtaatgcctg cgttaatgaa
    45841 cagctttcgt cctgtttctt acggttaagg cacaagtttg attgcgggaa attaagcacc
    45901 cgttccagtt cgcgaaacgc cgtttgccga tgcccggcaa agtcagcaat tgcccctcct
    45961 gccctactga ggatcgcgtc agtcacgttc gcgttatcca gttggcaaac gccagttttt
    46021 agaggattaa ataacggatc gggactggac gtatctgcct gggtatgtac ggttattgca
    46081 cagtcaggtg ccagcccggc ggcgaaggct tcgcctgttt tacgggtacg ttcgtcgaca
    46141 tcagcaataa ccgcgacctg acctggctgc gggcagccct tttttgccag caatccgtcg
    46201 gccaccagac gctggcgttg gtaatgtccg agataagcga ttaactcacc accgcgcggt
    46261 gtcagccaac ccagttttac cggccaggtt ggccatgcgt caggggtgac atcctgcatc
    46321 agttgcgtgg ccttggttgg tgcacgcaca ccgtgacgac tgacaatcac cacactttcc
    46381 agcttcagct ccggctcact ctgagcgaat gcagatttcg gtgttaacgg aatcagaaga
    46441 gataagaatg ggatcaagat cgctttcatc gatatgtttc cgcttttcat atccgacatt
    46501 attgattgcc tgatgcgacg ctaatgcgcc tcatcaggct taccttcgtt aacttttcaa
    46561 tcgcgggtcc agccatacca acacaagggt ggagagcgaa cacatcaaca aaatgccgac
    46621 gaaccaaagt aaataccaca ttgttcctgc tccttagtac agctcgtttt cgttacggcg
    46681 gagagtttct gtcgtcatgc gcccccacat tttgtagtag ctccagagag tgtagagcaa
    46741 cacaatgggc aaaaatatca gcacgattac caacataatg ctcagcgtca gctgactgga
    46801 agtgctgtcc cacaacgtca ggctggagat cggactcacg cttgacggca tgacaaaggg
    46861 gaacagcgtg atgcctgccg tgaaaatcac gccaaattgc atcaatgacg ccatcaaaaa
    46921 tccccaaccc gggcgaccac gataaatcgc catcaccgtc agcaatgggc agaagaaccc
    46981 cagcagcggg aagatccaca aaacgggcga ttcgacgaaa ttattcatcc aggcaccagg
    47041 tagcactgcc accagtttca ttaacggatt ggaaggaccg ttagcatcct gggcgagcag
    47101 tacaaagcca tcaataccaa cccacagcca gtaacccgcc agcaaaaagc acagcatcac
    47161 cagtagtgcg gcgcgtttgg tcgccagctg tgaacgcaga tgaatcacac caacagtttt
    47221 cagttgtaac cagacgccac cttgcaaaat caccatccca agactgagca atccacacaa
    47281 taaagggaat ggcgtcagca gttgccagaa gctgccgaga tattccacgc gtaattgcgg
    47341 tgtgaaagca aacggcacgc cgagcagcaa gttgccgaat gcaataccga agactaccgg
    47401 cggcaccaga ctgccgatga ccagtccggc atcccacatt ttacgccagc gtgcatcggc
    47461 gatttttccg cgataatcaa aggccagcgg gcggaagaac agcgagcaca gcaccagaat
    47521 catcgccaca taaaagccgg aaaacgccgc tgcatacact ctgggccagg cggcaaataa
    47581 tgccccacct gcgaggatca accagacctg attaccttcc cagtgcgcgc caacgctgtt
    47641 tatcactatc cgacgttcat catcattacg cgccaccagc ggcagcagac agccgatccc
    47701 catgtcaaat ccgtcggaga tcataaagac caccaggatc acgccaataa gcagccacca
    47761 gatgaagcgc aatgtttcat aatcaaacat gattttctcc tttacccctg ttgctgcgtc
    47821 ggttgttcac tctgcatcgc gctcggcccc agacgggcat atttctgcat caggtagact
    47881 tcggcgatta agaacagggt gtaaagccct acgatcatga tcagtgagaa agccagttgt
    47941 cctgtggtta aggcggagtg cgcggagtat gtcggtaaga tgtcttgtat cgcccacggc
    48001 tgacgaccaa actcggtcat aaaccatccg gcttcaatcg caatccacgg cagcggcaaa
    48061 ctccagagcg ccattttcag cacccagcga tgctggtcga ttttgccacg cagcgtctgg
    48121 acaagcgcaa tcagcatcac cagtagcagc agggaaccac agcccaccat gatgcggaaa
    48181 ctccagaata ccggcgcaac ctgaggtatc gcgccacgca tcgccgcctg gtactgtgcg
    48241 gctgtgacat gattcatatc cggcgcatag cgggagagca acatgccgta acccaggtcg
    48301 ccttccagct cccggaatgc ctgcaacaca tgcggctcac gattgccttg cgatatttcc
    48361 tgcattaaca gccacgccat acgtccgcgt tgcaagcgtg ggtaggtttc agccatcaaa
    48421 ttcttcagac ccggcacggg tttatctaat gaatgagtgg cgaggattcc tagcagcgcg
    48481 ggaattttga tagcaaaggc gttacgctct tgatcctgtt ccggccaggc aaccacatgg
    48541 aacggtgcag gtgcaggttc cgtttgccac tccccttcca tcgccgccag ttttaccggt
    48601 tgtacttgcg cgacttcata cgcagaactg tctccgagtt gcagcgtacc gataatcgcc
    48661 agagtaccga agacggaacc gatggcaaac gagcgtaatg cgacatcgcg ctcccgtccg
    48721 cgcagtaaat accaggcgct gatcgccata ataaacatag ccccggtcac gtatcccgcc
    48781 attacggtgt gcacaaattt cacctggctg accgggttaa agaccagctc gctgaaactg
    48841 gtcatctcca tacgcagggt gtcgatatca aaatgcgcac cggtcgggta ttgcatccaa
    48901 ccgttggcat tcaatatcca caacgcagag agatttgaac cgaacgccac cagccacgtc
    48961 accagcaggt gctggtattt attcagccgc tgccagccga agaagaacag cccgacaaag
    49021 gtggattcga ggaagaaggc cattaatgct tccatcgcca gcggtgcgcc aaaaatatcg
    49081 cccacatagt tggaatagaa tgaccagtta gtaccaaact gaaactccat ggtcaggccg
    49141 gtagccacgc caagagcaaa gttgataccg aagagcttac cccagaagcg cgtcatatcg
    49201 cggtagattg ttttgccggt gaccacgtaa atggtttcca tgatagccag caaaaaaatc
    49261 agccccaggg taaggggtac aaataaaaag tgatacagcg cggtcagagc aaactgccag
    49321 cgcgataaat caatgacatc ccacatgcgc actcctgtag gcgaagagag aaaaataacc
    49381 cgttaatggt agatctccct ctttatcctg aaactctacg accagaggta ttcgccgatt
    49441 gcgatattca taagcctgaa agcgatactt accgtctttt tacgtcggtg caccttccgc
    49501 cagccactgg catacctcgc taagccgctg cgcagagtcg accaaatctt ctggcgctgc
    49561 cagcaccact tccggtattg ggcagatttc ataactttcc agtaacgggc cttttaaggt
    49621 gtccgtacag cgtaaatgcc agacatggcg taaccccgtg gcgttgatat agctctcgcc
    49681 atagccaatg gtacgaatct gaatatttcc cggcccacag agacgtgaga gaaaaagccg
    49741 gtcagcttcg ctgatgggta attgcgtcag attaatgctg tgcggctgcg cgtcggggtt
    49801 acgtacatgt gccagtaatt catgggccaa cggtaggcca ttcatcaggc catcaatggg
    49861 cggcggtagc aacgagtctg ttggcaagac gttttgcgtt gccgcctgcc acaacgccag
    49921 cggtgcgcag ccagcctcca gtttgtcttc cagcaactgt tcgccgtgac gtctgcgcac
    49981 ccgccataat ccgcagaaga tcgcctcctg aatttcactt tcactgtcgt cagcctgctg
    50041 aatgcgcact gacacttccc cttccccgag aagcgtattg ataaagtgac gatcgtcggc
    50101 gttgagattt aacagatcga ctgccagcac ctcgcccaac ggcggttggc gttcgcttag
    50161 ttgatgttgt aactcgttta acagcgcaat cacctgcggg ctgtgagcac attgctccag
    50221 ggccgccata ctcggttcac cattcacccg acaggtgatc ggcagtggat tcatgctgaa
    50281 actgtcatcg ttcggttgcg ttcctggccc cagcagatgg aaaaaagttt cgctcatgaa
    50341 gcacgctcct gttgcggttc gacaagcccg cgcatcaggt ttatcagttc cgcccacggg
    50401 tgaataccat tcagcacgcc gcgatagttt ccgccggtaa acactaatgt ggcgggaaag
    50461 cgaaagacgc caaagcgatc gccgatggct tcgctctgct caagatcagc aatcgctacc
    50521 tgccatgtat agtcgggaaa ctcgcgcagt aattcgccaa tcattaccgg gttatcgctg
    50581 acttctggcg tgcgtttcgg atcgctgctt aataacacca cgccgtctgg cgcttgcgta
    50641 agccagtcgt caagacggca ttcactgacc ggcgtccagc cacgtgccag cattcgttgc
    50701 cacaacgcat taaatggtgt gtcgttgctc atccctgttt ctccagcctc atcctgtact
    50761 gacgcaagcg aacgccttcg taattttcca tcgacagaca gtcataattg agacagcgca
    50821 actcctcagt cggttgcggt acaattcccc aggccgccag ctgtaccagc gccgcctgtt
    50881 ccgcagcggg cagttgctcc cgcgccagtt cgctcaggct gccgccgtag tcgtcgagca
    50941 tcgcgggttg cagaccgacg agggcaatat atgctggcag atgtccgcgg atatctgcca
    51001 gcgccagcac ttcggagaaa ctgttctggt gcaggctcat tttcttcgcg ctgagataag
    51061 ctggaatgcg ttctccggca tacgttcgta gcgttccggg ttccagcccg taatcaatgg
    51121 catcgagaat caacagatga ctggcgcttt cgacataccc cagcaagttc agtccttggg
    51181 tgccgccatc gacaatctcc acatactcgg gccagtggta atgggcatac agccgttccg
    51241 ccacccgcac gccgaagcct tcatcggccc acagcaggtt acccagcccc attaccacca
    51301 cgcgttgctc gctcatgaac gctccttgtt acttattttg ccaaatttgt ggctacggta
    51361 gccgttgacc atagtggaga tcaccgtgtc gtcggacatg atgtcttcac gcagcgccat
    51421 gtagacatga ccgatcacaa acgcgccaat cagccacatc cccagccgat gccagctgtg
    51481 aatgtccatt gagttgccac ccgtccagta gaaaaattcc accacataac ggaacggcgc
    51541 aaaaatagcg tactggctgt gttcgctgta cagcgcaaag ccggtgatga tcataaaaac
    51601 tgacatcagg aaatagccga acatcgccgc ctgggcgatg gggttatggc ctatatcggc
    51661 actcggacgt tttgccagaa acagatacca gcggatttca taccacacgc cctgccacca
    51721 gcttttacgc cataccggca tgataaacag ctcgcgggag taacgattgc caacaaaagc
    51781 ccagtagatc cgcatcagca agaccacggt aaaaatcatc ccggcgctga agtgaattaa
    51841 cctgatgtag cccatataga acagatacgt cgcctcgccg ctgacggaag gtagcggctt
    51901 gccgataaag taaccggtga ccatcaacac cgccatgcat aacactgtca gccagtgcca
    51961 tatccgcacg ggtgcttcaa agacatagtg actgacaacg ttgtcgcttt tctgttgcat
    52021 gatgattctc cttcgctgtt aacgcacctg cacggagata agctcgctac cgtcgtcgcc
    52081 cagcacgtgt gttgaacagg cgaggcacgg gtcaaagctg tgcagagtac gcaggatctc
    52141 cagcggttgc tcggggatcg ccattttggt gttcatcagc gccgcttcat aagcgccaat
    52201 ctgccctttg ggatcgcgcg ggctggcgtt ccaggtggtc ggcaccacgc actggtagag
    52261 atcaatcttg ccatcgcgaa tggcagccca gtggcctaac gccccgcgcg gcgcttcggt
    52321 aaaaccgaca ccacggcact ctgtcggcca ggttgctggt tcccattttt ccgtggaagc
    52381 ggtggcaaga ttgccgtttt tcaggttggt catcagttta tcgaagaaat actgcaactt
    52441 acctgcggcc cactgcgctt cgtgcgcgcg acacaaaatg cggcccaacg ttgactggat
    52501 gccggaaagc ggcagtttca acgccgacat catacgatcg accgactcaa cggtcgcagc
    52561 atcgcctttg tgataagcga ttaacgtgcg tgccagcggc cccacttcca tcgcgttacc
    52621 gcgccagcgt ggcgctttga tccacgagta gcgttcctgt tcattcagct gctgaatgtt
    52681 ggtatcgctg cctttgacat cgccggggtt gtaccacggg tcggtaatgc catcgaacgg
    52741 atgacgcccc acctgatcgt tgggataacg ataccaggcg tggtcgacaa actcctgcac
    52801 ctgctgcgga tcaaccaaat ccactggcag cacattgttg aagtcgccgt taatcaccgc
    52861 gccgccaggc atcagcagac ttttctcgcc aaagtcgttg gcaatatccg ggaatgcgcc
    52921 gtagctgagg acacatttat cagaaagacc cgtgccgatt tcgctccacg gtttgttgaa
    52981 ctggccgatg gctaaagcgt cggggatcat cacgttgtta atgaaatccg ccgtacgggt
    53041 aatgatcgac tgcaccaggt tcaggcgttc catattgacc gccccgactg cgccgctttc
    53101 gtcaatgtta atggcgcaag gcatccctcc gacaatccag tttggatgcg ggtttttacc
    53161 accaaagacc gcgtggattt tgacaatttc acgctggaaa tcgagagctt cgagatagtg
    53221 ggcaaagccc atcaggttgg cttctggcgg cagtttgtac tgcgggtgcc cccagtagcc
    53281 attgcggaag atccccaact gcccgccttc aacgaatttc ttcaggcggt tttgtacgtc
    53341 gaagaaatag ccaggggatg atttcggcca tgaggagagg ctttgcgcca gttcggaggt
    53401 tttccgcggg tcggctttca gcgcatctaa cacatcgatc cagtccatcc cggcaagctg
    53461 atagaagtgc accagatgat cgtggcacca gagcgttgcc agcataatgt tgcggatgat
    53521 attggcgtta tccggcactt taataccgat ggcatcttcg atggcgtaaa ccgaagccag
    53581 ggcgtgtacg ccagtacaga cgccgcagat acgttcaacg aacgcccacg catcgcgcgg
    53641 gtcacgccct tgcaggatga tctccagccc gcgaaacatg gtgccgcagg agacggcatt
    53701 ggtgatcaca ttctgatcgt taatattcac ttcgcagcgc atgtggcctt caatgcgcgt
    53761 aatcgggtcg accaccaggc ggcgtccggc attgttgatg gtgtatccct gagtttcgta
    53821 ctgagtgctc atgcctgttt atcctcattg cctggctgat gttcggtttc tgtaggttgc
    53881 tggttatgac gtctgcgctg gtcaacggcg ctggcgactg cgtgcacacc aacagccgct
    53941 gccaccacgc caagcgcggt taaaccgacg gtgtcggcgg tggaatgggt acccatttgt
    54001 ggaatatcga ccacgcggct gtagaacgaa ccgcgatccc agaaaccatt ttccgcacaa
    54061 cccaggcagc cgtgacctga ctggatcggg aaggaaacgc catcgttcca gcgtgtggag
    54121 gaacaggcgt tataggtggt aggccctttg cagcccattt tgtacaggca gtaacctttg
    54181 cgggcagcgt catcatccca actctggacg aactctccgg cgtcgaagtg agcacggcga
    54241 tagcatttat cgtggatacg ctgaccatag aacatcaacg gacggcccat cctgtcgaca
    54301 tctggcaagc gatcaaaggt caccatgtaa gtaatgatgg cgctcatcac atccgggatc
    54361 ggcgggcagc caggtacttt gataataggt ttgtcggtga tgactttgtc gataggcgtt
    54421 gcctgcgtcg gattgggtcg cgcggcctgc acgcagcccc aggacgcgca ggttccccag
    54481 gcgataatcg cactggctcc ggcagcggca cgtttgagtt tctcaataaa cggtcgtccg
    54541 ctgctgatac agaacatccc ctgctcgccc agcggcggat taccttctac tgcgaggata
    54601 tatttgccat tgtattgcgt gatgatgtct tcaaagactt cttccgcctg ggttccggcg
    54661 gcagccatca aagtatcgtc ataatcgagg gaaatcaggg aaaggatgac gtccttcgcc
    54721 agcgggtgag cggagcggat aaaagattcg gtacagcagg tgcattccag accgtggatc
    54781 cataccaccg gaatgcgcgg tttgttctcc agcgcccagg caatctttgg tgccatcccc
    54841 gcgcctaatc ccagcgacgt ggcagccaga ctacaatatt tgagaaagct gcgccgggta
    54901 acgccctgac gccgcatggc ctggtaaaat gtttcctcgt tattcatatc gcacgtctct
    54961 tctccttgcg acaccggcag gacaccgggc gcttatggtc ggcaagcgac gtccctgtag
    55021 caaggcaaac catcgcttca cgtgagatgc tgaaaacgaa agctcatcct tctgcactgg
    55081 cgcacgtcgc cagaaagtaa tgttaataaa gcgtagtgaa acttttgcac aaaacaatac
    55141 aaactgtgtg gatttatctt ttagcgataa aaatggacct atttttcttt ggccgggcgg
    55201 tggggatgtt tagccggttg ctaaacgagt aaaagagaag gaattcatgg tgataaaatg
    55261 atcaatctgc tgttatttca cgctatcaat gcacttttgg ctgtttttca ggcaaacaaa
    55321 caaattaggg gtttacacgc cgcatcggga tgtttatagt gcgcgtcatt ccggaagtgt
    55381 ggccgagcgg ttgaaggcac cggtcttgaa aaccggcgac ccgaaagggt tccagagttc
    55441 gaatctctgc gcttccgcca aacaatcaag gggttaccaa atggtagccc ctttcttttt
    55501 ttgctcttag tatacacttg gtatactaat tggtatattt agctcaatac acactaggta
    55561 cattgttctc tttacgattt atcgctatac cgatgaatta tgaataaccc caatccatgc
    55621 atgacgtgtg gtgcctgttg tgcatttttc cgcgtctctt tttactgggc agaagctgac
    55681 gatgctggcg gaaaggttcc ggtcagtctc actgagcaaa tatccccctt tcatcgctgt
    55741 atgcgcggca ccagtcagaa aaatccccga tgtgtcgctc tggcgggaac tccgggtaaa
    55801 aacgcatatt gctctgttta caagaaccgt ccgtccacat gccgggaatt cgcaatgtcc
    55861 ggcgaaaatg gcatcgcgaa tgaagcatgt aaccgcgcac gggcaaaata cggacttccg
    55921 gaaatctgaa aaattatcca tatcaacagg atgcgccgga aacatgattt ccatcacatc
    55981 caggcctgca ctttccgtga cagtatttat tcgcatagta aatacttcac aagtgtataa
    56041 ttgactcagt tcatggggga ggtgatggtc ccccatcttt ttgtctgtta ttcctgtgcc
    56101 cagccaaccc tgtatgccag tatctcatcc gcactggtca gcttttccag ctctttcttc
    56161 atggtgcgct gccggacgtg gatttccatc cctttggtga acatcgcctg ctctgccgct
    56221 tcactcagtg ctataagctc ttctgctgtc acctccacat cattgttttc cgcatccgtc
    56281 cagaaaaacg cctccggcag tttccccgct ttcgctgccg ccaccgacgg ctcaagacgc
    56341 gtctgcgttg acttcccgta gtcccattta cgcccattgt gctcaaacat gtagttcgcc
    56401 gcttccatcg cattacgcca ggcgttaatt tcatcgccct tcatccctcg cgctttctcc
    56461 gcggtcagca gg
//
LOCUS       DAZKHK010000013        52555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKHK010000013 DAZKHK010000000
VERSION     DAZKHK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.013"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(45..1709)
                     /gene="glnS"
                     /locus_tag="ACHOS5_000836"
     CDS             complement(45..1709)
                     /gene="glnS"
                     /locus_tag="ACHOS5_000836"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP9775711.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1929..2690)
                     /locus_tag="ACHOS5_000837"
     CDS             complement(1929..2690)
                     /locus_tag="ACHOS5_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9775712.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(2692..3678)
                     /locus_tag="ACHOS5_000838"
     CDS             complement(2692..3678)
                     /locus_tag="ACHOS5_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HFP9775713.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAAAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(3671..4906)
                     /locus_tag="ACHOS5_000839"
     CDS             complement(3671..4906)
                     /locus_tag="ACHOS5_000839"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFP9775714.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEIHYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTVIPCQPVTVSQLSHLSYEQPWIGVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALWEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
     gene            5076..6233
                     /locus_tag="ACHOS5_000840"
     CDS             5076..6233
                     /locus_tag="ACHOS5_000840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFP9775715.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDALGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     AALGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            6237..7124
                     /locus_tag="ACHOS5_000841"
     CDS             6237..7124
                     /locus_tag="ACHOS5_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFP9775716.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANIAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            7184..7942
                     /locus_tag="ACHOS5_000842"
     CDS             7184..7942
                     /locus_tag="ACHOS5_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9775717.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            7978..9147
                     /locus_tag="ACHOS5_000843"
     CDS             7978..9147
                     /locus_tag="ACHOS5_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HFP9775718.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVALF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            9144..9614
                     /locus_tag="ACHOS5_000844"
     CDS             9144..9614
                     /locus_tag="ACHOS5_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP9775719.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            9628..10908
                     /locus_tag="ACHOS5_000845"
     CDS             9628..10908
                     /locus_tag="ACHOS5_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9775720.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNVAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(10997..12943)
                     /gene="nagE"
                     /locus_tag="ACHOS5_000846"
     CDS             complement(10997..12943)
                     /gene="nagE"
                     /locus_tag="ACHOS5_000846"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP9775721.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            13276..14076
                     /gene="nagB"
                     /locus_tag="ACHOS5_000847"
     CDS             13276..14076
                     /gene="nagB"
                     /locus_tag="ACHOS5_000847"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9775722.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKDL"
     gene            14136..15284
                     /gene="nagA"
                     /locus_tag="ACHOS5_000848"
     CDS             14136..15284
                     /gene="nagA"
                     /locus_tag="ACHOS5_000848"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9775723.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAISVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            15293..16513
                     /gene="nagC"
                     /locus_tag="ACHOS5_000849"
                     /gene_synonym="nagR"
     CDS             15293..16513
                     /gene="nagC"
                     /locus_tag="ACHOS5_000849"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP9775724.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            16561..17313
                     /gene="nagD"
                     /locus_tag="ACHOS5_000850"
     CDS             16561..17313
                     /gene="nagD"
                     /locus_tag="ACHOS5_000850"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP9775725.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            17568..19232
                     /gene="asnB"
                     /locus_tag="ACHOS5_000851"
     CDS             17568..19232
                     /gene="asnB"
                     /locus_tag="ACHOS5_000851"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP9775726.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDIEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPFNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            19613..19689
                     /locus_tag="ACHOS5_000852"
     tRNA            19613..19689
                     /locus_tag="ACHOS5_000852"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19647..19649,aa:Met,seq:cat)
     gene            19700..19784
                     /locus_tag="ACHOS5_000853"
     tRNA            19700..19784
                     /locus_tag="ACHOS5_000853"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19734..19736,aa:Leu,seq:tag)
     gene            19808..19882
                     /locus_tag="ACHOS5_000854"
     tRNA            19808..19882
                     /locus_tag="ACHOS5_000854"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19840..19842,aa:Gln,seq:ttg)
     gene            19917..19991
                     /locus_tag="ACHOS5_000855"
     tRNA            19917..19991
                     /locus_tag="ACHOS5_000855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19949..19951,aa:Gln,seq:ttg)
     gene            20007..20083
                     /locus_tag="ACHOS5_000856"
     tRNA            20007..20083
                     /locus_tag="ACHOS5_000856"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20041..20043,aa:Met,seq:cat)
     gene            20132..20206
                     /locus_tag="ACHOS5_000857"
     tRNA            20132..20206
                     /locus_tag="ACHOS5_000857"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20164..20166,aa:Gln,seq:ctg)
     gene            20244..20318
                     /locus_tag="ACHOS5_000858"
     tRNA            20244..20318
                     /locus_tag="ACHOS5_000858"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20276..20278,aa:Gln,seq:ctg)
     gene            complement(20472..21647)
                     /gene="ubiF"
                     /locus_tag="ACHOS5_000859"
     CDS             complement(20472..21647)
                     /gene="ubiF"
                     /locus_tag="ACHOS5_000859"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP9775727.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            21793..23217
                     /gene="miaB"
                     /locus_tag="ACHOS5_000860"
     CDS             21793..23217
                     /gene="miaB"
                     /locus_tag="ACHOS5_000860"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP9775728.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDHSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            23370..24410
                     /gene="ybeZ"
                     /locus_tag="ACHOS5_000861"
     CDS             23370..24410
                     /gene="ybeZ"
                     /locus_tag="ACHOS5_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9775729.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            24407..24874
                     /gene="ybeY"
                     /locus_tag="ACHOS5_000862"
     CDS             24407..24874
                     /gene="ybeY"
                     /locus_tag="ACHOS5_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9775730.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            24964..25842
                     /gene="corC"
                     /locus_tag="ACHOS5_000863"
     CDS             24964..25842
                     /gene="corC"
                     /locus_tag="ACHOS5_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP9775731.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            25867..27405
                     /gene="lnt"
                     /locus_tag="ACHOS5_000864"
     CDS             25867..27405
                     /gene="lnt"
                     /locus_tag="ACHOS5_000864"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP9775732.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVTVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNGIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(27532..29409)
                     /locus_tag="ACHOS5_000865"
     CDS             complement(27532..29409)
                     /locus_tag="ACHOS5_000865"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9775733.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSIFLHSSFSHLLMNMFALLAVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQAGIDNACHIGGLIAGGALGWLSARLVGQNRFVTEGGIIV
                     AVTLLLTGAIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFS
                     APIAKEKSVKHCGGCKDQGVRSLALSPDETLLYATSFEANALSVINVATGEIIQSITT
                     GAHPDNLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDEKTLLVPGAGRGNIVRINTITHQKEDFPAGDARGVVSAMGFRPKNGEIIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADDRKIFVMAKNRLIQMDRTRSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            29863..30771
                     /gene="gltI"
                     /locus_tag="ACHOS5_000866"
     CDS             29863..30771
                     /gene="gltI"
                     /locus_tag="ACHOS5_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP9775734.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSNTIFVVGTRLLTKKGSDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            30941..31681
                     /gene="gltJ"
                     /locus_tag="ACHOS5_000867"
     CDS             30941..31681
                     /gene="gltJ"
                     /locus_tag="ACHOS5_000867"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP9775735.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31681..32355
                     /gene="gltK"
                     /locus_tag="ACHOS5_000868"
     CDS             31681..32355
                     /gene="gltK"
                     /locus_tag="ACHOS5_000868"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP9775736.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32355..33080
                     /gene="gltL"
                     /locus_tag="ACHOS5_000869"
     CDS             32355..33080
                     /gene="gltL"
                     /locus_tag="ACHOS5_000869"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP9775737.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            33198..34133
                     /gene="rihA"
                     /locus_tag="ACHOS5_000870"
     CDS             33198..34133
                     /gene="rihA"
                     /locus_tag="ACHOS5_000870"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP9775738.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(34178..34660)
                     /gene="ybeL"
                     /locus_tag="ACHOS5_000871"
     CDS             complement(34178..34660)
                     /gene="ybeL"
                     /locus_tag="ACHOS5_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP9775739.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            34895..37477
                     /gene="leuS"
                     /locus_tag="ACHOS5_000872"
     CDS             34895..37477
                     /gene="leuS"
                     /locus_tag="ACHOS5_000872"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9775740.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            37492..38073
                     /gene="lptE"
                     /locus_tag="ACHOS5_000873"
     CDS             37492..38073
                     /gene="lptE"
                     /locus_tag="ACHOS5_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP9775741.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            38073..39104
                     /gene="holA"
                     /locus_tag="ACHOS5_000874"
     CDS             38073..39104
                     /gene="holA"
                     /locus_tag="ACHOS5_000874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9775742.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            39106..39747
                     /gene="nadD"
                     /locus_tag="ACHOS5_000875"
     CDS             39106..39747
                     /gene="nadD"
                     /locus_tag="ACHOS5_000875"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9775743.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSAQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPNVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            39771..40382
                     /gene="cobC"
                     /locus_tag="ACHOS5_000876"
     CDS             39771..40382
                     /gene="cobC"
                     /locus_tag="ACHOS5_000876"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP9775744.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFAMLRVLNSRAIGVENA"
     gene            40642..40959
                     /gene="rsfS"
                     /locus_tag="ACHOS5_000877"
     CDS             40642..40959
                     /gene="rsfS"
                     /locus_tag="ACHOS5_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9775745.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            40963..41430
                     /gene="rlmH"
                     /locus_tag="ACHOS5_000878"
     CDS             40963..41430
                     /gene="rlmH"
                     /locus_tag="ACHOS5_000878"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9775746.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            41461..43362
                     /gene="mrdA"
                     /locus_tag="ACHOS5_000879"
     CDS             41461..43362
                     /gene="mrdA"
                     /locus_tag="ACHOS5_000879"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP9775747.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            43365..44477
                     /gene="mrdB"
                     /locus_tag="ACHOS5_000880"
                     /gene_synonym="rodA"
     CDS             43365..44477
                     /gene="mrdB"
                     /locus_tag="ACHOS5_000880"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP9775748.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQITMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            44488..45576
                     /gene="rlpA"
                     /locus_tag="ACHOS5_000881"
     CDS             44488..45576
                     /gene="rlpA"
                     /locus_tag="ACHOS5_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP9775749.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            45716..46927
                     /gene="dacA"
                     /locus_tag="ACHOS5_000882"
     CDS             45716..46927
                     /gene="dacA"
                     /locus_tag="ACHOS5_000882"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP9775750.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            47037..47300
                     /gene="ybeD"
                     /locus_tag="ACHOS5_000883"
     CDS             47037..47300
                     /gene="ybeD"
                     /locus_tag="ACHOS5_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP9775751.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            47401..48042
                     /gene="lipB"
                     /locus_tag="ACHOS5_000884"
     CDS             47401..48042
                     /gene="lipB"
                     /locus_tag="ACHOS5_000884"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP9775752.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            48302..48622
                     /locus_tag="ACHOS5_000885"
                     /pseudo
     CDS             48302..48622
                     /locus_tag="ACHOS5_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            48863..49828
                     /gene="lipA"
                     /locus_tag="ACHOS5_000886"
     CDS             48863..49828
                     /gene="lipA"
                     /locus_tag="ACHOS5_000886"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP9775753.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(49928..50131)
                     /gene="tatE"
                     /locus_tag="ACHOS5_000887"
     CDS             complement(49928..50131)
                     /gene="tatE"
                     /locus_tag="ACHOS5_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP9775754.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(50260..51048)
                     /locus_tag="ACHOS5_000888"
     CDS             complement(50260..51048)
                     /locus_tag="ACHOS5_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP9775755.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            51141..51524
                     /gene="crcB"
                     /locus_tag="ACHOS5_000889"
     CDS             51141..51524
                     /gene="crcB"
                     /locus_tag="ACHOS5_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP9775756.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(51577..51786)
                     /gene="cspE"
                     /locus_tag="ACHOS5_000890"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(51577..51786)
                     /gene="cspE"
                     /locus_tag="ACHOS5_000890"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9775757.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(51961..52521)
                     /gene="pagP"
                     /locus_tag="ACHOS5_000891"
     CDS             complement(51961..52521)
                     /gene="pagP"
                     /locus_tag="ACHOS5_000891"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP9775758.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        12825 a        13687 c        13331 g        12712 t
ORIGIN      
        1 ttcttgccgg atgcggcgta aacgctctat ccgacaacgt aaacttactc gcctactttc
       61 gcccaggtat cacgcagccc aacggtgcgg ttaaataccg gtttttccgc cgtagaatgg
      121 cggctgtcga ggcagaagta accttcacgc tcaaactgga atgctttacc cgcaaccgca
      181 tctttcagcg acggttcagc aaagccctgt ttgatcacca gcgattccgg gttaatcacc
      241 gacaggaaat catccgcagc accagggttc ggcacgctga acagacggtc atacaaacgg
      301 atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa taacaccttt gactttacga
      361 ccatctgccg gatctttgct taaggtgtcg gcgtcataag tacagaagat ggtggtgata
      421 ttgccttcgg catctttctc gacgcgttca gccttaatga cgtaagcatt acgcagacgc
      481 acttctttac ccagcaccag acgtttgtac tgcttgttag cttcttcgcg gaaatcggcg
      541 cgatcgatcc aaatctcacc gctaaatggc acctgacggc tgcccatttc cggtttattc
      601 ggatggttcg gcatggtaac catttcgcct tcgccctggt agttttcgat aaccagtttc
      661 accggatcga taaccgccat tgcgcgcggc gcattttcgt tgagatcttc acggatgcag
      721 gattccagtg acgccatctc aatggtgttg tcctgcttgg tcacaccaat gcgtttgcag
      781 aactcacgaa tagaagccgc agtgtaacca cgacgacgca gaccagaaat ggtcggcata
      841 cgcgggtcat cccagccttc aacgtgcttg tcggtcacca gcaggttcag cttacgcttg
      901 gacatcacag tgtattccag attcaggcgc gagaattcgt actggcgcgg gtgaacagga
      961 atggtgatgt tgtccagtac ccagtcgtac agacggcggt tgtcctggaa ctcaagcgta
     1021 cacagagagt gcgtaatacc ttccagcgca tcgctgatgc agtgggtgaa atcgtacatc
     1081 gggtagatgc accacttgtt gccagtctgg tggtgttcag caaacttaat gcggtacagc
     1141 accggatcgc gcatcacgat aaacggcgaa gccatgtcga tcttcgcacg caggcaggct
     1201 ttaccttctt caaaaccgcc ggtacgcatt ttctcgaaca gcgccaggtt ctcttcaacg
     1261 ctgcgatcgc ggtacgggct gtttttaccc ggctgcgtca aggtgccgcg gtattcgcgg
     1321 atctgttccg gcgtcagttc atcaacgtac gccaggcctt tattgatcag ttcgatcgca
     1381 taggcgtgga gctgatcaaa ataatcggag gagtaacgga cgttaccaga ccagtggaaa
     1441 cctaaccact ctacgtcgtt tttgatcgaa tcgacatact cgatatcttc ttttaccggg
     1501 ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt cctgggcgat cccgaagttc
     1561 aggcagatag atttcgcatg gccaatatgc agatagccat tcggctccgg cgggaaacgg
     1621 gtgtgtactg tggtgtgctt accactggcc agatcttcat cgatgatctg acgaataaag
     1681 ttagtcgggc gggcttctgc ctcactcatc gtggattcct caaagcgtaa acaacgtata
     1741 acggcgtatg atcttataag cgggacaggc tgacaactgt tagttagcga aaaaatggtt
     1801 ttgcgtaaga tattggggcg ttttctgcgc tactgccagc agatcctcgc gctggaaacg
     1861 taaggccaaa cgtcgccagc ctgacgcatt tattaaattg ccggatgagc tggccgcaga
     1921 gtcatagatt agtcggctat gattaaacgc gtcgacaagg acgcaagcgt tctacgcgtc
     1981 tgcccatcaa gctgactatc cgtaataatc tctgtcagtt cattaagatg cgcgatatta
     2041 aacagcgacc atgaaccgta ttttgagctg tcagccagga gaattttgcg cgaagcatgc
     2101 gcgataagat cgcgttttag tgcggctttc tcttccgtgg gtgctgtaat acccttttca
     2161 agatcccagc tgttacagct taaaaatgcc acgtccggcc aggtattttg cagcaattta
     2221 cgaccatgtt cgccaatgca ggactggctg gagtcgtcga tacgtccgcc gataatcgtc
     2281 acttcaattt gcttgaattc agccaaaaaa agcgcgatat gcaggtcgct tgtgatcacg
     2341 cgaagcggta aatgtgtaat ctgccgcgca agttcaatca ttgtcgtacc ggcatcaagg
     2401 acgacggaat cccccgcttt gaccattgac gccgctgcgc tggcgatggc atgtttttcc
     2461 gccaggctgc gctgcatttt ctcatgggta gtcggttggg taggaataaa ccggttgagg
     2521 gtcacaccgc catgtgtacg gctgataaca ccctgctcat cgagttttat caggtctcga
     2581 cgaatcgtag ccggcgacgc attggttacc gccaccagct catcaacggt gaccagatta
     2641 tggcttttca ggtaatccat gatctgctct aaccggttat atcccttcat tttattctcg
     2701 cgaaatctgc attgctaact ggatggaaac tgccatgctc tcagacttgg cttttcctgt
     2761 ccaggcaata tcaaacgcgg tgccgtggtc ggcagaagtg cgaataaatg gcaacccggc
     2821 ggtgatattc accccatcat aaaatccaag cagttttaaa gggatatgcc cctgatcgtg
     2881 gtacatagcc actaccatat cgaatagccc ttcatggcat tgcataaaca cggtatctgg
     2941 cgggcacggg ccagtaacat caatatcttc ggcctgcatt gcttcaatcg caggcgcgat
     3001 aatctcgatt tcctcagtgc cgaataaacc atgttcccct gcatgtggat ttacacccgc
     3061 cacggcaata cgcggacgtt caataccaac acgtttcagg aagtggttcg caacacgaat
     3121 aaccgttttg accctttcac cattcaaggt atccaggaat ttacgcagtg agatgtgagt
     3181 cgaaatatgg ataaccttga gcttatcggt atagagcacc atcgcgtact ctttgctgcc
     3241 ggtaagatgg gccagcaatt ccgtatgccc cggatagtta tgcccgccaa gatgcagcgc
     3301 ttctttattc agcggcgcag tggctatggc tttcacctcg cctgataagg ccagagccgt
     3361 tgcacgtttg atacagcgat acgctaaatc gcctgccgca gcctgtacca ctccgggtgt
     3421 cagcgcatcg gggttagcca gcggctcatc catcacgtta atcacgccgg gagcaaaatg
     3481 ggcgtcgcac accttttgaa tgacgcgtaa ctctgcctgc ggcgtgatat tcatcgccat
     3541 aatacgtcgc agcgtgcgca cacagcccac caccaccacc ggggcaccag caagcccggc
     3601 ttcgctcagg gatttaataa tgatctccgg gccaataccc gccgggtcac ccatcgttac
     3661 cgcaataata ttattcacta cacttctcct cgataaaatt cagaacctga tgcaacgtgg
     3721 tctcatcgcc aaacccaccg gctttcgtca tcaccgaacg ggaccagaca ccgcccagaa
     3781 agtgaccgta tggaacgcat tgcgccacct tccccgtaat acgaaatccg gtcgcgccta
     3841 acgcactggc tgttgccatt gcgacatcac caccagagag ataaagcgcg tcgggcattg
     3901 tgcgaagcag aacttgccgg gtaagttcgc caagaaaccg acaaattttt tcccaaagtg
     3961 cagcgcgact catctgccaa cgctggcaaa gggtgtcgat ctgatgcctt gctacagaat
     4021 cgttacaggt gtggacaatg cagtggtcgc ctttctgcaa ggcctgcaca atacgcgcgt
     4081 catagtcact ggcattgcct gccagaatgt gttcaacatc gacgtatatc tgtgtgatac
     4141 gtgggtgtga atgaagtgtg gcgatctgcc gttgagcgat ttcgctcatt gaaccaataa
     4201 tggccagcac ggtgggtggc ggcataatcg cggagcgccg ggcaacggca tcacaaatac
     4261 cagcggagcc gaccagcagc agcggttgtt tagcctgcat cacagcggca gcgatgcgat
     4321 ccaggtcgct gtccgtctgc gcatctatca cgccaatcca cggttgctca taagacaaat
     4381 ggctgagctg agaaaccgta acgggctggc atggaatgac cgtctgcaac cggatgatgt
     4441 ccgcaattcg ggcactcaat accggggttt tcggatcgct ggcaaactcg gtttccgtta
     4501 tccgcacacc gtttacccaa cactcaccgg ccactgtggt ccttcccgcc tgaggaaatg
     4561 cgggggcaac cactgcgccc gttataccgc atgccttcat cagggcttca atttctgcgc
     4621 cgatattgcc tcgcagcgtg gaatctatct ttttaatcac gagcggcggg ttattcgcca
     4681 gagggagatg actcatcagc gaggttattt tcatcgccgc cagcttagga tccatcgccc
     4741 ggctgtcgct gttaatcacc catacgctgg catcaccgcg ataatgtatt tcaaaggcca
     4801 catcaactgt atgccccacc tgcgccaggc ttacccccgc gtcgttggct cccgtgaaat
     4861 catcggcaac gacgagaatg ctcggcttag tgattccctt ttccatcatc actccaggtt
     4921 gttttatgat aacaatgatt atattcaatc atgattgatt aaatgtgagc aagctcaagt
     4981 tattaatcac tcgaataaat tataaaatta gaccatgtta cgtaggctac ggcattttga
     5041 atgattgaat tcaatcacca gaagagaggt tatttgtggt aattatcaat agcacgatac
     5101 tgagcggcgc aggcgctatc ccttccctga cgtcgctctt acccgacatc agaaaaatgc
     5161 tgctggtcac tgaccgtaat attgcgcagc tcgacggtgt gcagcagatt cgcgccttac
     5221 tggaaaagca ctgcccgcag gttaacgtta tcgataatgt gcccgcagag cccacgcatc
     5281 atgatgtgcg ccagctaatg gatgcccttg gcgatgcctc ttttgatgtg gtggtcggga
     5341 tcggcggtgg cagcgtgttg gatgtggcga agctgctatc ggtgctttgc catccacaat
     5401 caccggggct ggatgcgctg cttgcgggtg aaaaaccgac tcagcgggtg caatcatggt
     5461 tgattcctac aaccgccgga accggctcag aagccacgcc gaatgcgatt ctggcaatcc
     5521 ctgagcaaag cacgaaggtg ggtattattt cccaggtgct gttaccagac tatgtggcgc
     5581 ttttcccgga actgaccacc agcatgcccg cgcatattgc ggcgtccacg ggcattgatg
     5641 ctctttgcca cttactggag tgttttaccg cgaccgtggc aaatccggtc agcgataacg
     5701 cggcgctgac tgggttaagt aaacttttcc ggcacattca acccgccgtg aacgatcctc
     5761 aggatctgcg cgcaaaactg gaaatgctgt gggcgtctta ctatggcggc gtagcgataa
     5821 cccatgcggg cacgcatctc gttcatgcgc tctcctaccc gttaggtggc aaatatcatc
     5881 tgccgcatgg cgtcgcgaat gccatcttgc tggcgccgtg catggcgttt gttcgcccct
     5941 gggcggtcga gaaatttgcc cgggtctggg attgcattcc cgatgcggaa accgccctga
     6001 gcgcggaaga aaaatctcat gccctggtga cctggttaca ggcattagtc aatcaactca
     6061 agctacccaa caatctcgcg gctctcggcg taccgccaga ggatattgcc tctctgagcg
     6121 aggcggcact gaacgtgaag cgccttatga acaatgtgcc gtgccagctt gatctacagg
     6181 acgtacaggc catttaccaa acactgtttc cgcaacatca atttaaggaa taaatcatgc
     6241 cgaaaaccat tgaaggtgta ttaaccgcta tcgtcacccc gtttaccgcg accggcagtc
     6301 tgaatatccc ggcgttaaaa gtgcaggtga atcgtcaact ggaagcgggt aacgcgattt
     6361 tttgcggcgg caccaacggc gaattttttg tgttgaatga acaggagaag ctcagcgtta
     6421 cgcaaacctg cgtggatgaa gtggctggtc gtgcgccggt cgtggcacac attggcgaga
     6481 tctctacgcg cgaaacgatt cgtctgggtc agcagattga aaaacttggc gtagatgccg
     6541 tgtcggtcat tgccccttac ttcgtaccgc tgaaacagga agagttgatt gcgcattaca
     6601 gcgcaattgc cgacgcactg agcattccga tgtttctcta taacatcccg gcccgcactg
     6661 gcaatacgat ccagccagaa accgcgcgtg ctctggcgtc gcaccccaat atcattggga
     6721 ttaaagacag cgccggaagc gacgaaagcc tgaagggttt tctcgatgcg gtgcgcgaca
     6781 ttgacggctt taatgtgctc aacggcccgg attcgctgat ccataaaggg tttgtcgagg
     6841 gatgttcagc ctgtatctct gggctcgcca acatcgcgcc gcgagaaatc aatgccatct
     6901 ggtctcgctt ccatgccggt gatattgaag ggtcttatga agcacaggag agtgtaacgg
     6961 gcctgcgtac cgatctttat aaagtagcat tctccccggc tgcggtgaaa aaagcgttgc
     7021 agcttatggg gcatgatgtt ggtgatagcc gctacgcggt taatttcacg cctgaacaga
     7081 tcagcgagat tcaacaaatt attcgtcatt ataacattaa ttaaattaac tctattatcc
     7141 agcccgctat aaagtttaga taaagagaat aaaggattac cccatgtttt tgtggtttat
     7201 gcagccagat atctggctca ctttaattac cctcaccttt ctggaaatta ttcttggggt
     7261 ggataatatt atttttcttt cactggtggt ggcaaaactt ccattatcac aacaaaatat
     7321 ggcgcgtaaa ctgggtttaa gctgcgcgat gattatgcgg atattattgc tgatatccat
     7381 tgcgtggctt tcacatatta cccagccgct ttttaccgtg ctgacgctgg agatttcgtt
     7441 ccgcaccttg attctgctcg ggggcgggct gttcctgatt tataaagcga tacaggagat
     7501 acgcgaagag ctgtcgcccg cgcaggaaga cgaacatggc agcggatcgc ggcaactgtc
     7561 gttgtgcggt gccattgtac agataatgct gctcgatatc gtgtttagcc tggattcggt
     7621 gatcaccgcc gtcggccttt ctcagcatat atttatcatg attgccgccg taatgattgc
     7681 cgtcggtgtc atgatgtttg cggcgaaaac aattggtgat ttcgttaacg ccacgccgtc
     7741 aataaaaata ctggcgttaa cctttttatt gtttgtcggt gtattgctgg ttgccgatag
     7801 tctcaacatt catattgcca aagagtatct ctacttcgcg atatttttct cattaagtgt
     7861 tgagacactc aatattatcc gtgaaagaaa aaataaaaaa gtttcgacat tgatcgaaac
     7921 gcatgaatcg cactcgcatt aataaatcta ttaccaactt taagatggag tctaactatg
     7981 tctgtagccc ttattcgtga cggcgtcatc cgtccatccg aaaacgattc gcatgtgctt
     8041 accgccatgc tgccctcgtc ctgcccgcaa aaccacgcgg cgaatattct gcctttgccg
     8101 gatggcgcgt taatgtgcgt ctggtttgcg ggaacgcagg aaggtattgc cgatatttct
     8161 gtctggggat cgcgattgcc cgctggcgga atgcagtgga gcgatgcggt caaactttcc
     8221 cacgatgaca cgcgttcaga gcaaaacccg gtgctgtttc tcgcgccgga taacgtgctg
     8281 tggctcctat ggaccgcaca aatatccggt aatcaggaca ccgccatcgt ccgctaccgt
     8341 aagtcagacg acctcggaca aacctggggc gagattgcca ccctgcttga taaacccggt
     8401 acgttcattc gacagccaat cacggtgctt gataacggca actggctgct gcccgtcttt
     8461 tattgccgta cccaacccgg cgaaaaatgg gtcggcaatg atgatatcag cgcagtgaaa
     8521 atttccgctg acggtggtca ctcctggcgc gatgtcgagg tgccgcaaag tcttggctgt
     8581 gttcatatga atatcacaat gctgcacaac ggtacgttgg ttgctctctt tcgcagccgt
     8641 tgggcagaca acatctatat cagccattcc gcggacaacg gcgagagctg gtcagtaccg
     8701 caagcaaccg aattgcccaa caacaattcg tctattcagg taacgacact tgccagcggc
     8761 gaactggcgc tggtgtacaa cgcgatgagt gcggcaggcg cagtagaacg tcgcgcgtcg
     8821 ctttatgacg aaatcgacga tggcgacgac agccgcaaag aaccgacagc cgtcggacgc
     8881 tccgctttct ggggggcccc acgcgcgccg atgacggtag cgatttctgc cgatggtggg
     8941 aaaagctggc cgtggcggcg caatctcgat gaaggcgacg gctactgcat gaccaacaat
     9001 tccctggaaa agctaaatcg tgagttctcc taccccagca tcaaacaaag cccggacggt
     9061 acgctacata tcgcttacac ctggtggcgt caggccatca agtacgtacg catctccccc
     9121 gagtgggtta aggggcaggc atcatgatta tcggtaatct caaccatttg tcgctggcgg
     9181 ggcttcctgc ctgggtgaga aatattctgc tgcgcccgga gtgttcgtta tcggcgttat
     9241 ccaccagaga ggacggtcgc tggcaacctg aaggatgtcg gtggttctgc acgctgggaa
     9301 cttcagacac gcaacccgcc gaactccgcc atacggagta tcaccattta tgggccgata
     9361 tacaggtggt gattactggt tgtgagggca ttaatgcggg aacgcggccc atcgctcgcg
     9421 aaaacgatga agaacgtaag cccgatctgt ttatcgcccc atcccctgaa aacagtgtcg
     9481 ccatcaccct acacgccggt gatttcgccg tctttatgcc tgaggaacct caccaggcgc
     9541 tgtgcgcaat tggcacggct acttcaatac gcaaagcggt ctttaaagta ccgcgcgaca
     9601 tgctggaagc ctaaaaggag agaaaaaatg acaatggaaa tgatcctggc attaggaata
     9661 ctggtcctga tgatcgtgct gatcatgtcg gacaaaatgc cttttggcgc accgccactg
     9721 cttgcgtgtt tattgctggt tgtatcggga ctttcgaccg ttcaacaggc ttttgccgga
     9781 ttcgtcaatc caagcgtggt gatgattgca gggtttatgg tggtcatggc ggcgctacaa
     9841 aaaacccggc ttatcagcaa cgttaaatct gcgatgataa gcctggtcaa taaaggcagt
     9901 taccgcagct atgggctgtt gttagtcatt gttatgctgg gtgccagtct tgccggcacc
     9961 ggagcgaccg gttattacgt gctaattctg tcgctggtat ccaccattcc gtacagtaaa
    10021 aagctaccaa catcgaaatt aatgatgccc ctgggatttg ccaccaacca cccgcttctt
    10081 cctatcaatc tcgcgcttct ctttggcgtt acggcgaccg tactcgaaac cgcggggttt
    10141 catcaggaga tttcgatggg gcgctttgcg ctggtgaatc tcatcatgtc agcggctttc
    10201 ctcgcatgga gtcttattgc ctaccgtttt cttccggatc atcccattgc ggatgcttcc
    10261 gaggatgcgc tggcggcacg cgaggaaacg tttaacgccc ttcctgcctg gaaagaatat
    10321 tgcactattg ccgcattcgc tgtcagcgtt atcggcatga tgctaatgaa cattctggga
    10381 aatgtcgcct tcgttattcc gggtcttgcg ggtgcgtttg ttctgatgat cgatgtactg
    10441 gacttcaaag aagttcgcga tcatatgggg gctccggtca ttttaatgat ggcgggggtg
    10501 atcggtgtag ctgatgcact ggcgggcacc ggctttacgg cgatggttgg cgatgccgtt
    10561 gcaggtgtgc tcggttctgg cgtctcaccg tttgtattta ttgtcgcctt tgctttgctt
    10621 accagtacct gcgccacctt cacgggttcg aatatggggt cagtatatat ttttgccccc
    10681 atcgccattg ccgcctgcac cagccttggg cttaacccga ccgctgccgc gattgcggtg
    10741 gtgatttctg gctggaatgg cggctatatg cctattgacg gaatgccagc catgatcctc
    10801 ggaatgggga aatacaaact tcccgaattc tgggtgttct ctgtaccaat gtatctcatc
    10861 cgcattctgg cgctttgtgc aggcgctgtt tttatcttcc caatgtaatc tcagacaaaa
    10921 aaagcggcag agaagatccc tgccgctttt tattgttgga tgcgacgctg ccgcgtcgca
    10981 tcagacataa agcagattac tttttgattt catacagcgg tgtttgaccc gccacaacat
    11041 ggccctgagc tttaatgata aggccgctga aatcgtcgat attgctgcaa accaccgggc
    11101 taatcatcga gcgagcgtta gcgttcaggt aatccagatc catttccaga atcggttgcc
    11161 ctgcgcttac ctgcgcgccc tcttccacca gacgtttaaa gcctttacct tccagcgcta
    11221 cggtgtcgat acccatatgg acgacgatct ccgcgccttt ttcggtttcc agacagaatg
    11281 cgtggttggt gttgaagatt ttcacgattg ttcctgcggc tggtgatacg acgattttat
    11341 ctgtcggttt caccgccaca ccgtcaccca ccgctttgct ggcgaatgct tcgtcaggaa
    11401 cctgatccag tgccacaaca tccccggtaa tcggggatac cagctccgcg atagataccg
    11461 cgtttggtac agcctgcggt tttgctacag gagcggcagt tgccggagtt gcttcagctg
    11521 acgcagcggc taccggacca cgggcaacga ctttcttcat cgcatcgccg atggattctg
    11581 ctttcgcgcc aacaatcacc tgaatagtct gtttgttcag tttcactacc ccagaagcac
    11641 ccagacgttt acacatcgca tcgttgacgc gagctgagtc aaccacggta aggcgcagac
    11701 gagtaataca ggcgtcaatg gctttcaggt tgtcagtacc gccaaccgca gcaatatagt
    11761 tggttgccag ttgattgaga ccttcttcag tattgctgtt agcctcttca gtaacgatct
    11821 cgtcttcttt atcttcacga cccggcgttt tcaggttgaa catgcggata accaaactga
    11881 acaccacgaa gtagatagcg aagaagacaa cccccatcac cagcagcatc cagacgttct
    11941 ggctggcggc cggcaggtta tacatcaacg cgtagtcgat agcccccgca gagaaagaga
    12001 agccggcatg gatacccagc agcgttgcca caaacaggct gataccggtc agcagtgcgt
    12061 gcaggaggta cagcagcggt gcaaggaaca tgaacaggaa ttccagcggc tcagtcacac
    12121 cggtcaggaa cgcagtaaca gcaacagaaa gcagcatccc gccaaccatc ggacgacgct
    12181 ctttcggtgc tgcgaagtac atcgccagcg ccgcacccgg cagaccgaac atcataatcg
    12241 ggaagaagcc ggacatgaac atccccgcgg tgccgtcacc ggcatagaag cggttgatat
    12301 caccgtggaa aaccgttccc gccgcgttgg tgaattcacc aatctggaac caggcgatgg
    12361 tgttcagcac ctgatgcaga ccagttggga tcagcagacg gttgatgaaa ccaaagatac
    12421 cggaacccag cgcgcccgca gaaacgatcc actcgccgcc tgcatggata gcgtgctgta
    12481 ccggtggcca gacgtaacca aaaatggccg ccagcaccag acagaagaag ccggtggcga
    12541 tcggcacaaa gcgtttgccg ccgaagaagc tcaggaagtc cggcagttta atatcggacc
    12601 aacggttata ggctgcgcca ccaaccagac cggtaatgat acccgccagt acacccatgt
    12661 taatttctgg gttgatggtc accatcgctt tggttaacac aaagtaacct accgcacccg
    12721 ccagtgccgc cgcacctgcg ctgtctttcg accagctgga tgccacgccg atggcgaaga
    12781 ttaatgcgag gttatcaaaa atcgcaccgc ccgcctgggc aataaacgca acgttaagta
    12841 aatctggctg accgaatcgc agcaacagcg ccgccaccgg cagcaccgcg atagggagct
    12901 gtaacgccct accgagtcgc tggaaaaaac ctaaaatatt catcttattc cccctacgag
    12961 aaccctattt ggctcgttta aagccgtatt tttattttgc tgcaaattgt actgccgatg
    13021 ttctgtaatc agattgttag atcatctgct acagagtgtg tgaaaattta attcgtatcg
    13081 caaattaaac gcgtgtcttt tgtgagtttt gtcaccaaat atcgttatca tcactccctt
    13141 ttactggcta aaccagaaaa cttattttat cattcaaaaa atcaggtcgg attgacgcct
    13201 gtctgcgcaa atccaggtta cgcttaaaga tgcctaatcc gccaacggct tacattttac
    13261 ttattgaggt gaataatgag actgatcccc ctgactaccg ctgaacaggt cggcaaatgg
    13321 gctgctcgcc atatcgtcaa tcgtatcaat gcgttcaaac cgactgccga tcgtccgttt
    13381 gtactgggcc tgccgactgg cggcacgccg atgaccacct ataaagcgtt agtcgaaatg
    13441 cataaagcag gccaggtcag ctttaagcac gttgtcacct tcaacatgga cgaatatgtc
    13501 ggtctgccga aagagcatcc ggaaagctac tacagcttta tgcaccgtaa tttcttcgat
    13561 cacgttgata ttccagcaga aaatatcaac cttctcaacg gcaacgcccc ggatatcgac
    13621 gccgaatgcc gccagtatga agaaaaaatc cgttcttacg gaaaaattca tctgtttatg
    13681 ggcggtgtag gtaacgacgg tcatattgca tttaacgaac cggcgtcttc tctggcttct
    13741 cgtactcgta tcaaaaccct gactcatgac actcgcgtcg cgaactctcg tttctttgat
    13801 aacgatgtta atcaggtgcc aaaatatgcc ctgactgtcg gtgttggtac attgctggat
    13861 gccgaagaag tgatgattct ggtgctgggt agtcagaaag cactggcact gcaggccgcc
    13921 gttgaaggtt gcgtgaacca catgtggacc atcagctgtc tgcaactgca tccaaaagcg
    13981 atcatggtgt gcgatgaacc ttccaccatg gagctgaaag ttaagacttt aagatatttc
    14041 aatgaattag aagcagaaaa tatcaaagat ctgtaattat tatccctgcc ctgacacctt
    14101 gctcagggca atatttttta aaatcggggg tcagaatgta tgcattaacc cagggccgga
    14161 tctttaccgg ccacgaattt cttgatgacc acgcggttgt tatcgctgat ggcctgatta
    14221 aaagcgtctg tccggtagcg gaactgccgc cagagatcga acaacgttca ctgaacgggg
    14281 ccattctctc ccccggtttt atcgatgtgc agttaaacgg ctgcggcggc gtacagttta
    14341 acgacaccgc tgaagcggtc agcgtggaaa cgctggaaat catgcagaaa gccaatgaga
    14401 aatcaggctg tactaactat ctgccgacgc ttatcaccac cagcgatgag ctgatgaaac
    14461 agggcgtgcg cgttatgcgc gagtacctgg caaaacatcc gaaccaggcg ttaggtctgc
    14521 atctggaagg tccgtggctg aatctggtaa aaaaaggcac ccataatccg aattttgtgc
    14581 gtaagcctga tgccgcgctg gtcgatttcc tgtgtgagaa cgccgacgtg attaccaaag
    14641 tgactctggc accggaaatg gttccagcgg aagtcatcag caaactggca aatgccggga
    14701 ttgtggtttc tgctggtcac tccaacgcga cgttgaaaga agcgaaagcc ggtttccgcg
    14761 cggggattac ctttgccacc catctgtaca acgcgatgcc gtatattacc ggtcgtgaac
    14821 cgggcctggc gggcgcgatc ctcgacgaag ctgacattta ttgcggtatt attgctgatg
    14881 gcctacatgt tgattacgcc aacattcgta acgctaagcg tctgaaaggc gacaaattgt
    14941 gtctggttac cgatgccacc gcgccagcag gtgccaacat tgaacagttc atttttgcgg
    15001 gtaaaacaat atactaccgt aatggacttt gtgtggatga gaacggtacg ttaagcggtt
    15061 catccttaac catgattgaa ggcgtgcgta atctggtcga acattgtggt atcgcactgg
    15121 atgaagtgct gcgtatggcg acgctctatc cggcacgtgc gattagcgtt gagaaacgtc
    15181 tcggcacgct cgccgcaggt aaagtagcca acctgactgc attcacacct gattttaaaa
    15241 tcaccaggac catcgttaac ggtaacgagg tcgtaactca ataagagaaa gtatgacacc
    15301 aggcggacaa gctcagatag gtaatgttga tctcgtaaaa cagcttaaca gcgcggcggt
    15361 ttatcgcctg attgaccagt acgggccaat ctcgcggatt cagattgccg agcaaagtca
    15421 gcttgccccc gccagcgtaa ccaaaattac gcgtcagctt atcgaacgcg ggctgatcaa
    15481 agaagttgat cagcaggcct ccaccggggg ccgccgcgct atctccatcg tcaccgaaac
    15541 ccgcaatttc cacgcaatcg gcgtacggct tggtcgtcac gacgccacca tcaccttgtt
    15601 tgatctcagc agcaaagtgc tggcagaaga acattacccg ctgccggaac gtacccagca
    15661 gacgctggaa catgccctgc tgaatgccat tgctcagttt attgatagct accagcgcaa
    15721 gctacgcgag ctgatcgcga tttcggtgat cctgccaggg cttgtagacc cggacagcgg
    15781 caaaattcat tacatgccgc atattcaggt agaaaactgg gggctggttg aagcactgga
    15841 agagcgtttt aaagtgacct gttttgtcgg ccacgatatc cgcagtctgg cgctggcaga
    15901 gcactacttc ggtgcaagtc aggattgcga agactccatt ctggtgcgtg tccatcgcgg
    15961 aaccggggcc gggattatct ctaacgggcg aatttttatc gggcgcaacg gcaacgtcgg
    16021 tgaaattggc catattcagg tcgaaccgct gggtgaacgc tgccactgcg gcaactttgg
    16081 ctgcctggaa actatcgccg ccaatgctgc cattgaacaa cgggtgttga atctgttaaa
    16141 gcagggctac cagagccgtg tgccactgga cgactgcacc atcaaaacta tctgcaaagc
    16201 cgcgaacaaa ggcgatagcc tggcctcgga agtgattgaa tatgtcggtc gtcatctggg
    16261 taaaaccatc gccattgcta tcaacttatt taatccgcaa aaaattgtta ttgctggaga
    16321 aatcaccgaa gccgataaag tgctgctccc tgctattgaa agctgcatta atacccaggc
    16381 gctgaaggcg tttcgtacta atctaccggt ggtacgttct gagttggatc accgctcggc
    16441 aatcggcgct tttgcgctgg taaaacgcgc catgctcaac ggtattttgc tccagcattt
    16501 gctggaaaat taatgtgctt ttatagtggc gcttattgtt gtcaatattc tgggtagtcc
    16561 atgaccatta aaaatgtaat ttgcgatatc gacggcgtgc tgatgcacga taacgtcgcc
    16621 gtaccgggtg cagcggaatt tttgcacggg attatggata aaggcctgcc gctggtgttg
    16681 ctgaccaact atccttcgca gaccgggcaa gatctggcga atcgctttgc cactgccggt
    16741 gtcgatgtac cggatagcgt gttttatacc tccgcgatgg cgaccgccga tttcctgcgt
    16801 cgccaggaag gcaagaaagc atatgtggtg ggcgaaggcg cactgattca tgaactgtac
    16861 aaagccggtt tcactattac cgatgtgaac cctgattttg tgattgttgg cgaaacgcgt
    16921 tcctacaact gggacatgat gcataaagca gcctatttcg tcgctaacgg tgcacgcttt
    16981 atcgccacca acccggacac ccacgggcgc ggtttttatc ccgcctgtgg cgcgttgtgt
    17041 gcagggatcg agaaaatctc cgggcgcaaa ccgttctatg ttggaaagcc gagtccgtgg
    17101 atcatccgcg cggcattgaa caaaatgcag gcgcattcgg aagaaacggt gattgtcggc
    17161 gataacttgc gtaccgatat tctggcgggc ttccaggccg gtctggagac gattctggtg
    17221 ctttctggtg tttcgtcact cgacgatatc gacagtatgc cgttccgccc aagttggatt
    17281 tacccatcgg tcgctgaaat cgacgttatc tgaaagcaac cacgcctcag ggcgtggttt
    17341 cttattttta cccatcatta aaatcacccc attcaataaa acccgtcaaa aattatcgat
    17401 ccatcaataa atatacccaa aagccctccc ctttttcatc aatcagcaat cgccattacg
    17461 ttttttattt ttccactgcc aaatcgcttg cgcaccgtgc tgctttgcgg cattttttta
    17521 acaagcaaac acaacacgca gtaaacacca ggttaacgga gaaggttatg tgttctattt
    17581 ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg taagaaagca ctcgagctgt
    17641 cacgcctgat gcgtcatcgt ggcccggact ggtccggtat ttatgccagc gataacgcca
    17701 tcctcgccca cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa cctctctaca
    17761 accaacaaaa aactcatgtg ctggcagtaa acggtgaaat ctacaaccac caggcattgc
    17821 gcgctgaata tggcgatcgt taccagttcc agaccgggtc tgactgtgaa gtgatcctcg
    17881 cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg tttgcctttg
    17941 ccctgtacga catcgaaaaa gatgcatatc tgattggtcg cgaccatctg gggatcatcc
    18001 cactgtatat gggctatgac gaacacggtc agctgtatgt ggcctcagaa atgaaagccc
    18061 tcgtgccagt ttgccgcacg attaaagagt ttccggcggg gagctatttg tggagtcagg
    18121 acggcgaaat ccgttcttat tatcatcgcg actggttcga ctacgatgcg gtgaaagata
    18181 acgtgaccga caaaaacgag ctgcgtcagg cactggaaga ttccgttaaa agccatctga
    18241 tgtctgatgt gccttacggt gtgctgcttt ctggtggtct ggattcctca attatttccg
    18301 ctatcaccaa gaaatacgcc gcccgtcgcg tggaagatca ggaacgctct gaagcctggt
    18361 ggccgcagtt gcactccttt gctgtaggtc tgccgggttc accggatctg aaagcagccc
    18421 aggaagtggc aaaccatctg ggcacggtgc atcacgaaat tcacttcact gtacaggaag
    18481 gtctggatgc catccgcgac gtgatttacc acatcgaaac ttatgatgtg accactattc
    18541 gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg
    18601 tgctgtccgg tgaaggttct gacgaagtgt ttggcggtta tctatacttc cacaaagcgc
    18661 cgaacgctaa agagttgcat gaagagacgg tgcgtaaact gctggccctg catatgtatg
    18721 actgcgcgcg tgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc
    18781 tcgacaaaaa attccttgat gtggcgatgc gcattaaccc acaggataaa atgtgcggta
    18841 acggcaaaat ggaaaaacac atcctgcgtg aatgttttga agcgtatctg cctgcaagcg
    18901 tggcctggcg gcaaaaagag cagttctccg atggcgtcgg ttacagttgg atcgacaccc
    18961 tgaaagaagt ggcagcgcag caggtttctg atcaacagct ggaaactgcc cgcttccgct
    19021 tcccgttcaa cacgccgacc tcaaaagaag cgtatctgta ccgggagatc tttgaagaat
    19081 tgttcccgct tccgagcgcc gctgagtgcg tgccgggcgg tccttccgtc gcttgttctt
    19141 ccgctaaagc gattgagtgg gatgaagcgt tcaagaaaat ggacgatccg tctggtcgcg
    19201 cggttggcgt tcaccagtcg gcatataaat aagacgtgta atattgcccc ggagaaatcc
    19261 ggggcaattt cagttactgg ggcgcttgtt gattctgata tgcagtaagg aaatgttccg
    19321 ggtttgccac atcgctgttt gacaactcat tactgtaggc cagtatttca tagattaatt
    19381 tcccaccact attgttgaat tccgaatttc atcctgtatg acaaaagaat ggctgtaggg
    19441 tggggaaaaa acacaaagta aacaataatt gacgaatata gcgccatgct gttcgcaacc
    19501 taaccaaaca gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc
    19561 tatacgcata atgcgccccg caacgccgat aaggtatcgc gaaaaaaaag atggctacgt
    19621 agctcagttg gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg
    19681 tagccaccat cttttttttg cgggagtggc gaaattggta gacgcaccag atttaggttc
    19741 tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc accattcacc agaaagcgtt
    19801 gtacggatgg ggtatcgcca agcggtaagg caccggtttt tgataccggc attccctggt
    19861 tcgaatccag gtaccccagc catattcttc gaataagcgg ttcaccgccc ggttattggg
    19921 gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg
    19981 taccccagcc atcgaagaaa caatctggct acgtagctca gttggttaga gcacatcact
    20041 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccaaattctg aatgtgtcga
    20101 atatgttcgg caaattcaaa aaccaatttg ttggggtatc gccaagcggt aaggcaccgg
    20161 attctgattc cggcattccg aggttcgaat cctcgtaccc cagccaattt attcaagacg
    20221 cttaccttgt aagtgcaccc agttggggta tcgccaagcg gtaaggcacc ggattctgat
    20281 tccggcattc cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcttcggc
    20341 gagctttttg cttttctgcg tatattcaat gccgaatgcg atgttgactc gtcttatcct
    20401 tcaaagtcgg atgcgacgct gccgcgtctt atccgaccta cggttggcat gcatccagca
    20461 atgttgtgag gctacaaccc taacgcatat ttcagcgcct tacgtttcaa cacgccagca
    20521 cgctccgccg ccatcaaacc aagattacgc acaaaacgca acggtggcag attattgctg
    20581 aatccggcat aaaacagatc cataccactt tgcatcatga agttatccgc catacgccgc
    20641 atctggtaac gcttgagcac aggataactg gcccacgctt cgccgtagct gcgggcgttc
    20701 accagaacgt caatcagggc atcgacatca cgataaccaa gattcactcc ctgtcccgcc
    20761 agcggatgga tggtatgcgc ggcatcgccc accagagcaa gccctggttg cacatactgc
    20821 aacgcatggc gacgtatcag cggaaacgca ccagcggcaa gcggtgtcac gtaacccaga
    20881 cgcgacggga aatgcttcgc gatttccgcc tgtagctgcg ccatattcat attctgcaac
    20941 tggcgaatgc gcgccggaga gtcataccac accagcgatg cccagttatc aaacaacggc
    21001 agaaacgcac gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc
    21061 tcacactgta cgctaatcaa catacacgac tgcgcatact gccaggcatg aacgccaatt
    21121 cccgccatct gtcgcacctg cgaatttgcg ccgtcggcac caatcaccag cttcgcgagg
    21181 atcgtttcac cgcccttcag ttccagctcc tgaagatcat tatggcgatg cagtgcaatc
    21241 agcgaggttg gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg ccacaacgcc
    21301 tgttgcagga cagtgttttc caccatgtag ccaagcaacg gtagcttaag ttcagcggcg
    21361 tcaaacacca catgcgccgt ttcccactcc cacgtttcca gtctgcggta aggatggcaa
    21421 cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac cgaagccgca
    21481 ctaatcgccg agatccgcac gtccggctgg ctgtcagcga caaacggtgc aggttgggca
    21541 tgttcgataa ccattaccga aaatccgtgc tgagccagtc ccagcgccag tgcgccgccg
    21601 accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct
    21661 gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac
    21721 tggtcacaac ggcagcaaag cattacacta tgcgccctgc attcctggct actatttcgc
    21781 aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag
    21841 tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgac
    21901 gtggcggaag aagcggatgt gctgctgctg aacacctgct caatccgcga gaaggctcag
    21961 gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg
    22021 attatcggcg tcggcggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc
    22081 cactatgtcg atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac
    22141 tccgtgcgcg gcgaccacag cccggttgta gatatcagct tcccggaaat cgagaagttt
    22201 gaccgtctgc ctgaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc
    22261 tgcaataaat attgcaccta ctgcgtagtg ccttacaccc gtggtgaaga ggtaagccgt
    22321 ccgtccgacg atattctgtt tgagattgcc cagcttgctg ctcagggcgt gcgtgaagtt
    22381 aacctgctcg gccagaacgt gaacgcctgg cgcggtgaga actacgacgg caccaccgga
    22441 tcgtttgccg atctgctgcg tctggttgcg gcgatcgacg ggatcgatcg tattcgcttt
    22501 accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtaccg cgacacgccg
    22561 gagctggtga gcttcctgca tctgccggta cagagcggtt ccgatcgcat tctgaacctg
    22621 atgggacgta cccatacggc gctggagtac aaagcgatca tccgtaaact gcgtgcggcg
    22681 cgcccggata ttcagatcag ctctgacttc atcgtcggct tccctggcga aaccaccgaa
    22741 gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt
    22801 atcttctctg cacgtccggg tacaccagcc gccgatatgg ttgatgatgt tccggaagaa
    22861 gagaagaagc agcgtctgta tattctgcaa gagcgcatta atcagcaagc gatggcatgg
    22921 agccgccgga tgctcggcac cacccagcgc attctggtag aagggacttc acgtaagagc
    22981 atcatggagc tttccggtcg cactgaaaat aaccgcgtgg ttaacttcga gggcacaccg
    23041 gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc
    23101 ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgcg tggcagaaac accggaatcg
    23161 gtgattgccc gtacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaattc
    23221 tcaggcccgc cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa
    23281 tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct
    23341 tccggcggca tacagataag aggaacggtt tgaacataga cactcgcgaa atcaccctgg
    23401 agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaaac
    23461 aactcgaacg ccgtctcggc atcgaaatca atcgccgcga taaccacttt aaactgaccg
    23521 gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgatacag
    23581 ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag
    23641 aagcgcgggt actggagcaa agcgcggaga gcgtgccgga gtacggcaaa gcggtcaata
    23701 tcaaaaccaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca
    23761 atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc
    23821 tggcagtggc tgcggcagtt gatgccctgg agcgtcagga aattcgccgt attctgctga
    23881 ctcgtccggc agtagaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga
    23941 aagtagaccc gtatctgcgc ccgctgtacg acgcgctgtt tgaaatgctg ggctttgaga
    24001 aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc accgctggcc tatatgcgtg
    24061 gtcgtacgct gaacgacgcg tttatcattc ttgatgaaag ccagaacacc accatcgaac
    24121 agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg
    24181 tcacacagat cgacctgccg cgtaatacca agtctggttt gcgtcatgcc atcgaagtgc
    24241 tggccgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc
    24301 cggtggtggc gcgtatcgtt aacgcctatg aagcctggga agaagccgaa caaaaacgta
    24361 aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat
    24421 cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca
    24481 gacatggctg aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt
    24541 ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac
    24601 caacgtgctc tccttcccgt ttgaagtacc gcctggcatg gaaatgtcgc tactgggcga
    24661 tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga
    24721 ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat
    24781 cgaagatgac gaagcagaag aaatggaagc catcgaaaca gagattatgc ttgctctggg
    24841 ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg
    24901 tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa cccatacgac
    24961 gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc
    25021 tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg
    25081 ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa
    25141 ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg
    25201 attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat tgagtccgcc
    25261 cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg
    25321 aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta
    25381 cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc
    25441 tctcagcgtt accacatggc gatcgtcatt gacgaattcg gtggggtttc cggtctggtg
    25501 accattgaag acatcctgga actgattgtt ggtgagattg aagacgagta tgacgaagaa
    25561 gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc actggcttct
    25621 attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaggt tgacactatc
    25681 ggtggtctgg tgatgcaggc atttgggcat cttccggcgc gtggcgaaac catcgacatc
    25741 gacggttacc agttcaaagt ggcgatggcc gacagtcggc gtattattca ggttcatgtc
    25801 aaaatcccgg atgactcacc ccagccgaag ctggatgaat aaaaccgaaa ctggatagat
    25861 aactacatgg cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta
    25921 ttattcggtg cctgcggaac gctggccttc tctccttacg acgtctggcc tgcggcgatt
    25981 atttcgctga tggggcttca ggcgttgacc tttaaccgcc gtccactcca gtctgccgct
    26041 attggctttt gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc
    26101 atcgcgacct ttggcggaat gcctggcccg gttaacatct tcctggtggt actgctggcg
    26161 gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa
    26221 accacctggc tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac cgagtttctg
    26281 cgcggttggg tgctgactgg cttcccgtgg ttacagttcg gctatagcca gattgatggc
    26341 ccgttaaaag ggctggcacc gataatgggc gtggaagcca ttaacttcct gctgatgatg
    26401 gttagtggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct ggtggtgacc
    26461 gtcgtgctgt ttgcccttcc cttcccgctg cgttacatcc agtggtttac cccgcaaccg
    26521 gagaaaacca ttcaggtttc gatggttcag ggcgatattc cgcaatcgct gaaatgggac
    26581 gaaggccagc ttcttaatac gctgaagatt tactacaacg caacggcacc gctgatgggc
    26641 aaatcatcgc tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa
    26701 ccgttcctta aagctctgga cggtgagttg cgcgataaag gtagctcgct ggtcaccggg
    26761 attgtcgacg cgcgtctcaa taaacagaac cgctacgata cctacaacac catcatcaca
    26821 ctgggtaaag gcgcaccgta cagctatgaa tcagccgatc gctataacaa aaaccatctg
    26881 gtgccgtttg gcgagtttgt cccactggag tcgatactgc gtccgttagc accgttcttt
    26941 gatttgccga tgtcgtcatt cagccgcgga ccatatattc agccaccgct gtcgttgaat
    27001 ggcattcaac ttactgcggc catttgctac gaaatcattc tcggcgagca agtgcgcgat
    27061 aacttccgcc cggataccga ctatctgctg accatctcca acgatgcgtg gtttggtaag
    27121 tctattggtc catggcagca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc
    27181 ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca gggtgagatt
    27241 caggcgatga tcccgcagtt cacccgcgag gtgttgacca ctaacgtgac accaaccacc
    27301 ggactcacac cgtacgcacg aaccggcaac tggccgctgt gggtgctgac ggcgctgttt
    27361 ggttttgccg ctgtactgat gagtctgcgt gctcgcaaac gttaatgtgc ctgatgcgcg
    27421 acgcttatca ggcctacgcg atttttacaa tatgttgaat tttcgcgatt ttgtaggata
    27481 aagcgtactt tttcaacacg ctctggggca tgattatcat gccctttttt cttacggttc
    27541 agggatacaa gccacatcgc catattgggg atgccgaata attgcctgcg gatcaagcga
    27601 gcgcgtcctg tccatttgga ttaacctgtt cttcgccata acaaatatct ttctatcatc
    27661 cgctgaaaaa cgaagatgat tcacgtagct ggcgggataa acgccaataa tctgcccggc
    27721 attaatattg agcaggatga cataaccttg caggccatat gacaccaacg ctaaatactg
    27781 accgtccggg ctaataccct caacggaata aacgctcctg ctacaccatt gcgtcataat
    27841 gctggcttgt tgatcctcag ctcttatacg tgaaatcccc tggctatcgg caaaaataat
    27901 ctcaccattt tttggtcgga atcccatcgc agaaacgaca ccacgcgcat cacctgcggg
    27961 aaagtcttct ttctgatgcg tgatggtatt gatccgcacg atgttacctc tgcctgctcc
    28021 cggaaccagt aatgtttttt catccgggga aagtgccata acccaggcgc ctggtttacc
    28081 gctcgtcccc gtcccgtcgt atttctccag tgggatatcc gccacatgct gataagtcac
    28141 cagatcaatc gctgacacac tattactggt gcgattcatc acccaggctt ttgtgccatc
    28201 acgcgagagg ataaggttgt caggatgtgc gccggtggta atcgactgaa taatctcccc
    28261 cgtcgccacg ttaatgaccg ataatgcatt cgcttcaaat gaagttgcat aaagcaacgt
    28321 ttcatccggg cttaatgcca gcgatctgac gccctgatct ttacagccac cacaatgttt
    28381 gactgacttt tctttcgcta tgggcgctga aaaggtatgc aggattttct tttgtccgag
    28441 gtcgaaaaca acaatgctgt tcgtgttttc aattgcggca tacaacgtat taccatcccg
    28501 actgatcgcc atatcatgga tatcggcaat ctcagcgagt aaatcaccac tggcctgctc
    28561 acgacttacc ggagcggata atgtttccct gagggcgttg cgctcctcca ctaactgctg
    28621 ctttttttgt cgtcgctctt gttcaatctc ctgcggataa aacgcttcac gcaggctttg
    28681 cctgacctgt aaaactgatt catcaatctg ctgctgcaca agccagatag ccccggtcag
    28741 aagaagagtg accgcaacaa taatcccgcc ttccgtaacg aatcggtttt gcccaactaa
    28801 acgtgcgctc agccaaccca acgcgccccc cgcaatcagg ccgccaatgt ggcaagcgtt
    28861 atctattcct gcttgtcggg taccatacaa cagtgtcagc gccaccatac ccaacagagg
    28921 aaatactcgc cgctggtttt tatggtgtgt gcccgcacct gaagcaagct gtgttgctat
    28981 cgcagcgccc gctattccca taattgcccc ggatgcccca acgctgatga ctatctgctc
    29041 actttcgcgt aacgcataac aggcgctgat cagcccacca aagatgccgg agaataacca
    29101 gataatcagg agtcgccatt tccccagtat ccgttccgct actgtcccca ccgccaggag
    29161 tgcaaacata ttcatcagca aatgggaaaa actactgtgc agaaaaatac tgctcaataa
    29221 gcgccaggat tcatccgaca acgtgagaga agcaacgtta gcgccgagat ggatgagtgc
    29281 ggattgatcg acttcttcgt aactcatcca ttgtgcgagg taacaaaata tcccaacaca
    29341 gagcgcaaaa aggatcaggg ttattgcggg taattgaaca tttaacggct taaccgaaga
    29401 tgcagacata aggtgattct tccttgaaaa gactagtaaa aagcattttc ccgtgtgtag
    29461 ttatcatccc ggcaaacgtc ccacaccgaa aagttaacac tgtccgaaat atgacttttt
    29521 caaactgaaa ttacaatcgt aattaattca cccctaagta ttttttggca tgtaagttgc
    29581 tttatttact gtgtaagcaa tgaaatcagc cgcaacgcaa ccgggttgca ccaccacaaa
    29641 tcagcggtag caccaaaaag gtgcggttta agaattttgc ttcggaatgg tgcggcgcac
    29701 ctcgcaaaaa taaacaataa aacatcaata tctttacatt tatccaaact aaatgttacc
    29761 taacaaatac aacactgcac aataaagtcg cagatgataa caacacaaac actcacaacg
    29821 ggtatccatg cgtttttaac gtagaagata aaggagttgg atatgcaatt acgtaaacct
    29881 gccacagcaa tcctggccct ggcgctttcc gcaggactgg cacaggcaga tgacgccgcc
    29941 ccggcagcag gcagcacgct ggacaaaatc gccaaaaacg gcgtgattgt cgtcggtcac
    30001 cgtgaatctt cagtaccttt ctcttattac gacaatcagc aaaaagtggt gggctactcg
    30061 caggattact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accagactta
    30121 caggtgaaat tgattccgat cacctcacaa aaccgtattc cactgctgca aaacggcact
    30181 ttcgattttg aatgcggttc taccaccaac aatgccgaac gccaaaaaca ggcggctttc
    30241 tctaacacta ttttcgtggt cggtacgcgc ctgctgacca aaaagggcag cgatatcaaa
    30301 gattttgccg acctgaaagg caaagccgta gtcgtcactt ccggcaccac ctctgaagtt
    30361 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
    30421 cacggtgact ctttccgtac cctggaaagc ggtcgtgccg ttgcctttat gatggatgat
    30481 gctctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acaactggga aatcgtcggc
    30541 aagccgcagt ctcaggaagc ctatggttgc atgttgcgta aagacgatcc gcagttcaaa
    30601 aagctgatgg atgataccat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
    30661 gataagtggt tcaaaaatcc aattccgccg aaaaacctga acatgaattt cgaactgtca
    30721 gacgaaatga aagcactgtt caaagaaccg aatgacaagg cactgaacta attacaagaa
    30781 ccaggggcgg aaattccagc cctctcgatt gttacgtagc acggacagac tatacgcctg
    30841 gtggtcgttc cccatcgggc ctgaaaaccg caatacgctg ggtaacaatc ttcgagggta
    30901 gcagttaacg ctgctaccct cttttttctg gagtagattt atgtctatag actggaactg
    30961 gggtattttt ttacaacaag ccccgttcgg caacaccacc tatcttggtt ggatctggag
    31021 tggctttcag gtgacgatcg ctttgtcgat ctgcgcctgg attatcgctt tcctcgtcgg
    31081 ttcatttttc ggcattttac gtaccgttcc aaaccgtttt ctctccggtc tgggtacgtt
    31141 gtatgtcgaa ctgttccgca acgtgccgtt gattgtgcaa ttctttacct ggtacctggt
    31201 gatcccggag ctgctgccag agaaaatcgg catgtggttt aaggcagagc tggatcccaa
    31261 tattcagttt ttcctttcat ccatgctctg cctggggctg tttaccgccg cccgtgtttg
    31321 cgaacaggta cgcgcagcta ttcagtcgct gccgcgtggg caaaaaaatg ccgcgctggc
    31381 gatgggtctg acgctaccgc aggcctaccg ctacgtactg ctgcctaatg cttatcgcgt
    31441 tatcgtcccg ccgatgacct cagagatgat gaacctggtg aaaaactccg ccatcgcctc
    31501 gactatcggt ctggtggata tggcggcgca ggcgggtaaa ttgctcgatt actcggccca
    31561 cgcctgggag tcgtttaccg ccattacgct ggcgtatgta ttgattaacg cttttatcat
    31621 gctggtgatg acgctggttg aacgtaaagt tcgcctgccc ggcaacatgg ggggcaaata
    31681 atgtacgagt ttgactggag ttccattgtt ccttccctgc catatctgct cgacgggctg
    31741 gtgatcacgc tgaaaatcac cgtcacggca gtggtgattg gtattttgtg gggcaccatg
    31801 ctggcggtga tgcgcttgtc cagctttgcg cccgtagcgt ggtttgccaa agcctacgtt
    31861 aacgtattcc gctcgattcc tttagttatg gttttgctgt ggttttacct gatcgtaccg
    31921 ggttttctgc aaaacgtgct gggattatca cccaaaaatg atatccgttt aatttcggca
    31981 atggtggcgt tttcgatgtt tgaagcggcc tactattcag agattatccg tgctggtatt
    32041 caaagtattt ctcgtggtca gtcgagcgcc gcgctggcgt tgggaatgac tcactggcag
    32101 tcgatgaaac tgattattct gccgcaggcg ttccgcgcga tggtgccgct gctgctcact
    32161 cagggcatcg tactgtttca ggatacctca ctggtgtatg tgttatctct ggccgatttc
    32221 ttccgtaccg cctcaaccat tggtgagcgc gatggtacgc aggttgagat gatcctgttt
    32281 gccggatttg tttatttcgt tattagcctt agcgcatcgt tgttggtcag ctacttgaaa
    32341 agaaggacag cataatgatt accctgaaaa atgtttcaaa atggtatggt cactttcagg
    32401 tgctgaccga ctgctcaacc gaagtgaaaa aaggcgaagt tgtggtggtt tgcggcccgt
    32461 ctggttccgg caaatcaacg ctgattaaaa ccgtcaacgg cctcgaaccg gtgcagcaag
    32521 gtgaaatcac cgtcgatggc atcgtggtta acgacaagaa aaccgatctg gcgaaactgc
    32581 gttcccgcgt cgggatggtg tttcagcatt tcgagctgtt cccccatctg tcgattatcg
    32641 aaaacctgac cctggcgcag gtgaaagtgc ttaaacgcaa taaagcgccg gctcgtgaaa
    32701 aagcattgaa attgctggag cgcgtcgggc tttccgcaca tgccaataag tttccggcgc
    32761 agctttccgg cggccagcag cagcgtgtgg cgatcgctcg cgcattgtgc atggatccta
    32821 ttgccatgct gttcgacgaa ccaacgtcgg cgctggatcc ggagatgatc aacgaagtgc
    32881 tggacgtgat ggtcgaactg gcgaacgaag gaatgacgat gatggtggtg acccacgaaa
    32941 tgggctttgc ccgtaaagtg gcgaatcggg tgatctttat ggacgagggt aaaattgtcg
    33001 aagactcgcc gaaagacgct ttcttcgatg atccgaaatc ggaccgcgca aaagacttcc
    33061 tcgcaaaaat cctgcattaa tcactctggc gcgcatactc aattgcgcgc catctcacgc
    33121 tttctcgttt cgtccctcgt atctgttaca tcccggcgtt acccttgtcg caaagaaaca
    33181 cacaacaagg agcaacaatg gcactgccaa ttctgttaga ttgcgaccca ggtcatgacg
    33241 acgctatcgc aatcgttctc gccctcgcct caccagagct tgatgtcaaa gcaattacgt
    33301 cttccgccgg aaaccagaca ccagaaaaaa ccttacgcaa tgttctgcgt atgctgacct
    33361 tgcttaatcg caccgatatt ccggtagcaa gcggtgcggt aaaaccgtta atgcgtaatt
    33421 tgattattgc ggacaacgtg cacggcgaaa gcggtctcga cggcccggca ttaccggaac
    33481 cgacgttcgc accgcaaaac tgtacggcgg tggagctgat ggcgaaaacg ctgcgtgaaa
    33541 gtgaggaacc tgtcaccatt gtttctaccg gaccgcaaac taacgttgcc ttgctgctca
    33601 atagccaccc ggaactgcat agcaaaattg cccgtatcgt gattatgggc ggcgcaatgg
    33661 ggcttggtaa ctggacgcct gcggctgaat ttaatattta cgttgacccg gaagcggcag
    33721 aaattgtctt ccagtcagga attccggtgg tgatggccgg tctggatgtt actcacaaag
    33781 cacaaatcca cgttgaagac actgagcgtt tccgcgcgat tggcaaccct gtttcaacca
    33841 ttgttgccga actgctggat ttcttcctcg aatatcataa agacgaaaaa tggggctttg
    33901 tcggcgcacc actgcatgac ccatgcacca tcgcctggct gttgaaaccg gagttgttta
    33961 ccactgttga gcgctgggtt ggcgtggaaa cacaagggaa atatacccag ggtatgacgg
    34021 ttgttgatta ttattatctg actggcaata aaccgaatgc caccgtaatg gtcgatgttg
    34081 atcgtcaggg ctttgttgat ttactggctg atcgtctgaa attttacgct taatcactcg
    34141 ccggatgaca tcacgccatc cggcaccact catttcatta cggctcgaac ggacgtctct
    34201 ggaactgatc atgaccacat ttcgggcata gcgtcagcac ttccggtgta tagatcggga
    34261 gatggaagtg acatttctcg cagaccagat ttcccagccc taccacttct ccgctgtgat
    34321 aaaccccgtg atgattgagg tcctggaaaa cttctcgcca ttcaagctgc gttttatcgg
    34381 tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag acgctgtcag
    34441 actcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca cgtctgacag
    34501 ctcgcgtcag ctcatcgacc tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc
    34561 gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt tcgctcaatg
    34621 acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct cccgggtcgt
    34681 taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc ccacaaactc
    34741 tgtaaattaa tgtatgaata atccgggcat catttcaacg tcgagtgcgc gaaaggctgt
    34801 tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacatca acgctacatt
    34861 tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc gcccggaaga
    34921 gatagaatcc aaagtacagc ttcactggga tgagaagcgc acatttgaag taaccgaaga
    34981 cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg gtcgactaca
    35041 catgggccac gttcgcaact acaccatcgg tgacgtgatc gcccgctacc agcgcatgct
    35101 gggcaaaaac gtcctgcagc caatcggctg ggacgcattt ggtctgcctg cggaaggcgc
    35161 ggcggtgaaa aacaacaccg cgccagcacc gtggacgtac gacaacatcg cgtatatgaa
    35221 aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg cgacctgtac
    35281 gccggaatac taccgttggg aacagaaatt cttcaccgaa ctgtataaaa aaggcctggt
    35341 atataagaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac tggcgaacga
    35401 acaagttatc gacggctgct gctggcgctg cgataccaaa gttgagcgta aagagatccc
    35461 gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc tggataaact
    35521 ggatcactgg ccagacaccg ttaaaacaat gcagcgtaac tggatcggtc gttccgaagg
    35581 cgtggaaatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt acactacccg
    35641 cccggacacc tttatgggtt gtacctacct ggcggtggct gcgggtcatc cgttggcaca
    35701 gaaagcggcg gaaaataatc ctgaactggc cgcctttatt gacgaatgcc gtaacaccaa
    35761 agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc
    35821 ggttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg tattgatgga
    35881 atacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact acgagtttgc
    35941 ctctaaatac ggcctgaaca tcaaaccggt tatcctggca gctgacggct ctgagccaga
    36001 tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg agtttaatgg
    36061 tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga tgggcgttgg
    36121 cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgtcagc gttactgggg
    36181 cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccgaccc cggacgacca
    36241 gctgccggtg atcctgccgg aagatgtggt gatggacggc atcaccagcc cgattaaagc
    36301 agatccggaa tgggcaaaaa ctaccgttaa cggtatgcca gcactgcgtg aaaccgacac
    36361 tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc cggagtacaa
    36421 agaaggtatg ctggattcca aagcggctaa ctactggctg ccggtggata tttacattgg
    36481 tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca aactgatgcg
    36541 tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt
    36601 gctggcagat gccttctact atgttggtga aaacggcgaa cgtaactggg tttccccggt
    36661 tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg
    36721 ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca acggtatcga
    36781 cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc
    36841 ttctccggct gatatgactc tcgaatggca ggagtccggc gtggaagggg ctaaccgctt
    36901 cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg ttgcggcact
    36961 gaacgtcgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat
    37021 cgctaaagta accgatgata tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat
    37081 tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg atcgcgccct
    37141 gatgcaggaa gcgctgctgg cggtagtccg tatgcttaac ccgttcaccc cgcacatctg
    37201 cttcacgctg tggcaggaac tgaaaggcga aggcgatatc gacaacgcgc cgtggccggt
    37261 tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg ttaacggtaa
    37321 agtccgtgca aaaatcaccg ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc
    37381 tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta aagtgattta
    37441 cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag cgtgcgatat
    37501 ctggcaacat tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg
    37561 cgtgacacca cgcaggttcc ttccactatg aaagtcatga tcctggactc aggcgatccg
    37621 aacgggccat taagccgtgc ggtgcgtaat cagttacgtc tgaatggtgt cgagttgctt
    37681 gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg
    37741 aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat gatcatgacg
    37801 gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc caaagtcttc
    37861 cgttcgttct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca agacatgatc
    37921 gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc aagcatccgt
    37981 gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac tccggcaacg
    38041 cctgcacgcg tctccaccac gctgggtaac tgatgattcg gttgtacccg gaacaactcc
    38101 gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt
    38161 tattgcagga aagccaggac gctgttcgtc aggtcgctgc ggcgcaaggg ttcgaagaac
    38221 accacacttt ttccattgat cccaacaccg actggaatgc gattttttcg ttatgccagg
    38281 ccatgagcct gtttgccagt cgacaaacgc tattgctgtt gttacccgaa aacggaccga
    38341 atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac gatctactgt
    38401 tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc
    38461 ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag ctgccccgct
    38521 gggttgctgc gcgcgcaaaa cagctcaact tagaactgga tgacgctgca aatcaggtgc
    38581 tctgctactg ttatgaaggt aacctgctgg cgctggccca ggcactggag cgtttatcgc
    38641 tactctggcc ggacggcaaa ctgacattac cgcgcgttga acaggcggtg aatgatgccg
    38701 cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt
    38761 tgcatattct tcagcaactg cgtctggaag gcagcgaacc ggttattttg ttgcgcacat
    38821 tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat acgccactgc
    38881 gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt
    38941 taaatcgctt aagccagccg cagttacgtc aggccgtgca actcctgaca cgaacggaac
    39001 tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc
    39061 tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa atctttacag
    39121 gctctgtttg gcggcacctt tgatccggtg cactatggtc atcttaaacc cgtggaaacg
    39181 ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt tcctccgcat
    39241 cgtccacagc cggaagcgaa cagcgcgcag cgtaaacaca tgcttgaact ggcgatagct
    39301 gacaagccac tgtttaccct tgatgaacgc gagctaaagc gcaatgcccc ctcttacact
    39361 gcgcaaacac tgaaagagtg gcggcaggag caaggaccga acgtgccgct ggcgtttatt
    39421 attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac gatactcgac
    39481 aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc acaaccacaa
    39541 tatcagcaat ggctggagga tcatttgaca cataacccgg aagatcttca ccttcagcct
    39601 gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac catcatccgc
    39661 gaacgtttgc aaaatggtga atcgtgtgag gatttattac cggaaccggt actgacttac
    39721 attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg atgcgactgt
    39781 ggttaattcg tcatggcgaa acgcaagcga atgtcgatgg tctgtacagc ggtcatgcgc
    39841 ccactcccct gaccgcgcgc ggtattgagc aagcgcaaaa tctgcatacg ctgctacatg
    39901 atgtttcctt tgacctggtt ttatgcagtg aactggaacg ggcacagcat accgcgcgac
    39961 tggttcttag tgaccgccag caccccgttc atatcatccc tgaactcaat gaaatgtttt
    40021 ttggcgactg ggagatgcga catcatcgtg acctcatgca agaagatgcc gaaaactata
    40081 gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga tttcaggcat
    40141 tttcgcaacg tgtggaacga tttatcgcaa ggcttagtga atatcagcac tatcagaata
    40201 ttttagtcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta attggcatgc
    40261 ctgccgagtc tatgtggcat ttccgcgttg accaggggtg ttggagcgcc attgatatca
    40321 accaaaaatt cgcgatgcta cgcgtcctca atagccgtgc catcggggtc gagaatgcat
    40381 gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg ggcaggctga
    40441 tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg ttttacaaaa
    40501 atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa gccgattcgg
    40561 cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt tttatctatt
    40621 tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt tatcgacaaa
    40681 attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcagggcaa atccagcatc
    40741 accgactgca tgatcatctg tacgggtacg tccagccgcc atgttatgtc cattgctgat
    40801 cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac
    40861 agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat gcaggaagag
    40921 agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc
    40981 gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta cctgcgtcgt
    41041 tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggaaaacg cggcaagaat
    41101 gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa
    41161 aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca gttagccgct
    41221 gagctggaac gctggaagct ggatggtcgc gacgtcagtc tactgattgg cgggcctgaa
    41281 gggttgtcgc ctgcctgtaa agcggcggcg gagcagagct ggtcgctgtc ggcacttacc
    41341 cttccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc
    41401 accaccaacc atccttatca ccgtgagtga taagtgagct ttgagtagaa aacgcagcgg
    41461 atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg
    41521 gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc caacctgtat
    41581 aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa ccgcattaag
    41641 ctggtgccta tcgcacccag ccgcggcatt atctacgacc gtaacggtat ccctctggct
    41701 ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa cgtgcagcaa
    41761 acgctggacg ctttgcgcag cgtggttgat ctgaccgatg atgatattgc tgcattccga
    41821 aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa
    41881 gtacaagtag ctcgctttgc cgtcaaccag taccgttttc ctggcgtcga agttaaaggc
    41941 tataaacgtc gttactatcc ttacggttca gcgttgaccc acgtcatcgg ctatgtgtcg
    42001 aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc caactatgcg
    42061 gcaacgcatg atatcggtaa gctgggcatt gaacgttact atgaagacgt gctgcacggt
    42121 cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa
    42181 gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa actccagcaa
    42241 tatattgaaa cgctgctggc gggtagccgc gcagctgtgg tagtcaccga tccgcgtaca
    42301 ggtggggtgt tggcgctggt ttccacgcct agttatgacc caaacctgtt tgttgacggt
    42361 atctccagca aagattattc cgccttgctg aacgacccga atacaccgtt ggtgaaccgc
    42421 gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgttgc ggtttcggca
    42481 ttgagtgccg gggtgatcac gcgcaatacg acgctgtttg accccggctg gtggcaactt
    42541 ccaggttcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc
    42601 acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta cgatatgggg
    42661 atcgatcgcc tctccgaatg gatggggaaa ttcggttatg gtcattacac cggtatcgac
    42721 ctggcggaag aacgttccgg taacatgcca acccgcgaat ggaaacaaaa acgctttaaa
    42781 aaaccgtggt atcagggtga caccattccg gttggtatcg gtcagggtta ctggacggcg
    42841 acccctatcc agatgagtaa ggcactgatg atcctgatta atgacggtat cgtgaaggtt
    42901 ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt acaaccgcat
    42961 gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga cggtatgtac
    43021 ggtgttgcta accgccctaa cggtacggcg cataaatatt ttgccagcgc accgtacaaa
    43081 attgcggcga aatccggtac cgctcaggtc ttcggtctga aagcgaacga aacctataat
    43141 gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt tgcgccatac
    43201 aacaatccgc aagtggctgt cgccatgatt ctggagaacg gtggcgcggg tccggcggtt
    43261 ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa caccgatctg
    43321 cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg gataatccga
    43381 ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg atcttactgg
    43441 cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat attggcatga
    43501 tggagcgtaa aatcggccag attacgatgg gtctggtcat catggtggtg atggcgcaaa
    43561 ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt attattttgc
    43621 tggtggcggt agatgctttc ggtgccatct ctaaaggcgc tcaacgctgg ctggacctcg
    43681 gtattgttcg ttttcaaccg tcggaaattg ccaagatagc cgtaccgttg atggttgcac
    43741 gctttatcaa ccgcgacgtt tgcccacctt cattgaagaa tacagctatc gcgctggtgc
    43801 tgatatttat gcccacgctg ctggtggctg cacagcctga cttgggaaca tcaatcctcg
    43861 ttgcgctttc cggcctgttt gtactgttcc tctctggcct tagctggcgt ctgattggcg
    43921 tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg atgcatgatt
    43981 accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc ggcgcgggct
    44041 atcatattat tcaatctaaa attgctattg gctccggcgg attacgcggc aaaggctggc
    44101 tgcacggtac tcagtcacag cttgaatttc tccccgaacg ccataccgac tttatcttcg
    44161 cggtactggc ggaagagctg ggattagtgg gcattttgat tctgctcgct ctttacattc
    44221 tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt ggtcgcgtca
    44281 tggctggcgg cttaatgctg atattattcg tttatgtctt cgtaaatatt ggtatggtaa
    44341 gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga ggatcggcgc
    44401 taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac aggaaaatgt
    44461 tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg
    44521 gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt aagtgtaccg
    44581 cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcagaccc gcgtttcgaa
    44641 ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg
    44701 caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc cgaacccggc
    44761 agtaacctga cggcctctgg cgaagctttc gatccgacac agttgacggc ggcccatcca
    44821 acgcttccaa tccccagcta cgccagaatc actaacctgg ctaacgggcg aatgatcgtg
    44881 gtgcgcatta atgatcgcgg cccttatggc aacgaccgcg tgatttcgct ttctcgcgcg
    44941 gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc gattattgtc
    45001 gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc caaacagact
    45061 tacgccctgc cagcacctcc cgatttaagc ggtggcgcgg gaacaagttc agtgcctgtc
    45121 ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc
    45181 gcgccggtaa ccagcagcgg tttcctcggc gcaccaacga ccttagcgcc tggtgtactg
    45241 gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagctccgtc gacaacgcct
    45301 gcaacctcac ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt
    45361 atggtgcaag tcggggccgt tagcgatcag gctcgtgcgc aacagtacca acagcaactg
    45421 ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg gcggatccag
    45481 cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa
    45541 gcccaattac agtcatttat taccaccgcg cagtagcgta aaacaggcat ctgatgtgtc
    45601 aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc cggtagtgca
    45661 tttgctatag tagggcactt tttttaattc catcacggat gtcgtagttc agaccatgaa
    45721 taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc
    45781 ctttatctct gctgcacatg ccgatgacct gaatatcaaa accatgatcc cgggtgtacc
    45841 gcagatcgac gcggagtcct acatcctgat tgactataac tccggcaaag tactcgccga
    45901 acagaacgct gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt
    45961 tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca ctatcggcaa
    46021 cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc
    46081 gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga
    46141 tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat
    46201 gaacagctac gttaacgccc tgggcctgaa aaatacccac ttccagacgg tacacggtct
    46261 ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcgttgat
    46321 ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat
    46381 tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg acggcatcaa
    46441 aaccggacac actgacaaag caggttacaa ccttgttgct tctgcgactg aaggccagat
    46501 gcgcttgatc tctgcggtga tgggcggacg tacttttaaa ggccgtgaag ccgaaagtaa
    46561 aaaattgcta acctggggct tccgtttctt cgaaaccgtt aacccactga aagtaggtaa
    46621 agagttcgcc tctgaaccgg tttggtttgg tgattctgat cgcgcttcgt taggggttga
    46681 taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag ccagctatgt
    46741 gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gtactatcaa
    46801 cttccagctt gatggcaaaa cgatcgaaca acgcccgctg gttgtgctgc aagaaatccc
    46861 ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt
    46921 tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa gcatcagtaa
    46981 aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga gctgacatga
    47041 aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa gttatggggc
    47101 aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg
    47161 actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta tctatcacta
    47221 tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc aaaatcgata
    47281 ttgtccgcat ggttctgtaa ctcgcttctc cgttacccga ctcctggtcg ggtaactccc
    47341 ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg agacgccgtt
    47401 ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga gccaatctcc
    47461 caggctatgc atgaattcac cgatacccgc gatgatagta cccttgatga aatctggctg
    47521 gtcgagcact atccggtatt cacccaaggg caggcaggaa aggcggagca cattttaatg
    47581 ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta tcacgggccg
    47641 gggcaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa
    47701 ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg tatagaagcg
    47761 catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg ctcactgggt
    47821 ttacgtattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa tatggatctt
    47881 tcaccatttt tacgtattaa tccttgtggg tatgcaggaa tggaaatggc taaaatatca
    47941 caatggaagc ccgaagcgac aactaataat attgcaccac gtttactgga aaatatttta
    48001 gcgctactaa acaatcctga cttcgaatat attaccgctt aattccatac atcaatggcc
    48061 caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc cttcgcgttt
    48121 gtatattttc tttatcactt gcagaaatgc gatgctacgc cttgctccgt agcccaccta
    48181 attgattaac tcattgaaat aattgaccat ggtgagcaaa agcgttccgt cccaattaga
    48241 cataagatta ttgtaatttt tataaatatt atttttccat gaataaaatt tgagtaagtg
    48301 cgtggatagt aataatcaaa ttgaaccctg tttaagtaga aaatcgtcag aaggtaaacc
    48361 acaaatattt acaaccttac gaaatattga tcttactctt ctgactattt tgaagttgca
    48421 tatgtccata aaggaatcgt taatgcagcg aaagtgctta atctgccccc cctcggtcgg
    48481 agcccaggtc cttcctgtca tctatcgggc gattaaaact cactatccac agcttttact
    48541 gcgcaacccg cccatcggcg gcgcggaaaa ccaactctgt cagtttcaaa ccgatctcat
    48601 caccgataac atgttttgca caggaccata gatgttatcc gtaatgcatt ttgaaaaagt
    48661 attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac aacaatttta
    48721 cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact gccagtcgtt
    48781 aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat
    48841 tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg gtgttaaata
    48901 ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag agcgcgaagc
    48961 cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta cacgtatcca
    49021 gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg
    49081 ccctaacctg gcggaatgct ttaaccacgg cacagcaacg tttatgatcc tcggcgctat
    49141 ttgtacccgc cgttgcccgt tctgtgacgt tgcccacggt cgcccggtag ctcctgatgc
    49201 caatgaacca gtgaaactgg cgcagacaat tgccgatatg gcgctgcgtt atgtggttat
    49261 cacctccgtt gaccgtgatg atctgcgcga tggcggtgcc cagcactttg cggattgcat
    49321 tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc cggatttccg
    49381 cggtcgtatg gatcgtgctc tggatattct gactgcaacg ccaccagatg tgttcaacca
    49441 taacctggaa aacgtaccgc gtatttaccg tcaggtacgg cctggcgcag attacaactg
    49501 gtcgcttaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga ccaagtctgg
    49561 tctgatggtg ggactgggtg aaaccaatga agaaattatt gaggtaatgc gcgacctgcg
    49621 ccgtcatggt gtgacgatgc taacgctggg gcaatatttg cagccaagcc gccatcacct
    49681 gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg aagcgctggc
    49741 gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc acgccgattt
    49801 gcaggcgaaa gggatggaag ttaagtaatc ctgtaataat tacttacatc tgtctgataa
    49861 aaaacccgct ttttgaagcg ggtttttgta tcgaacaaga tattgaggga gcgtcctgct
    49921 cgccacgtca ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt
    49981 tcgccgcagc atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt
    50041 ctccgcccag cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta
    50101 ccagcagttt ggtaatacta atctcaccca tagatacctt cttgacatat aaccggcaac
    50161 agaacgcgcc ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca
    50221 atctgtaaca aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc
    50281 aaagcgacgg ttgtttaaga cgggcagttg cgcgcgtacc tgacgcacac gttcgggcgt
    50341 cacttccgcc ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg
    50401 atctataatc cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata
    50461 acaggtggta tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg
    50521 cccacgaacc caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag
    50581 cgccagctct ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccttccac
    50641 ctccagtaac ggagctattt cattaccagc atcaacatgg cgtgattcct gaatggcaaa
    50701 tgcatcatag agatgcagtt tggcataacg ggcgacgatg tttcctgcct gtagcgccac
    50761 cagcatattc cacgcgcgcc ccggtgttga aggaacatga atcgtcagaa ttgtcgtcat
    50821 catgttacgt ttactttctc gccgtaaacg gtcgaggaat tcgccttcca gcagctgtgc
    50881 tgatttaacc gaaagatccg catcatgatc atcacgcgcc agtaatgctt ccggcaggac
    50941 aaacagcgaa acctcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt
    51001 cttttcccac acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg
    51061 tcagagtcgt aaaattcgcc aacggcgatt attcggcgtt acacttatca ctcatacaaa
    51121 tcaaatagca ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta
    51181 cgggaagcgt ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc
    51241 cgttggggac gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct
    51301 ggttcagcag gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt
    51361 gtggcggtct aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg
    51421 gccgctttgg ctgggcgtta ctgaacgttt tcgtcaatct tttggggtct tttgccatga
    51481 ccgcactggc tttctggctg ttttcggcct caaccgcaca ctaaaaacca aaaaaacccg
    51541 ctgattaagc gggttttgaa ttcttgctga cgtatcttac agagcgatta cgtttgcagc
    51601 agaagggcct ttggcaccgt tagtgatttc gaactctacg cgctgacctt cagcaagagt
    51661 tttaaaacca ttagtctgga ttgcagagaa gtgtacgaac acgtctttgc tgccgtcttc
    51721 cggagtaatg aaaccgaatc ctttggactc attaaaccac ttaacgttac ctttaatctt
    51781 agacatcaaa attaccttta catgaaaaat agacacaaat gctgtgtcgg ttaccagtac
    51841 accaattgtg gtacgctttg tccagtgcaa ctttactaaa aagtgataaa agtcgcgtta
    51901 tttttgatgt taattatttt agtggttgat tttaaaggat ttactaaaac ttcatttgtc
    51961 tcaaaactga aagcgcatcc aggcaaagta cacattgcca ttgttgtagg tacccggaat
    52021 gtaggtcatc tgaaacgtcg cggggccata ccccacagag gccaatggca gtagaaccgg
    52081 aagagggatg taattccagt tatcgcgcgc cgttacgcca gcggtaaatc ccaggcctaa
    52141 atgaaaattt tcatccgcca gcggtcgcca ggtactttcc catccgtatc cggcaatcgg
    52201 ttcccatttg ttccacgagt ccttaaatgc catggcatac aggccatgcc agtttccttt
    52261 ttcatcccaa cgcgactgac caaaaccgcc gccccacggt cgctcgttat agcgatcggt
    52321 tttttctttg tcgtaagcaa aacgtgcatg ccaggtgatg gcaggaatat ataaatcata
    52381 atgttctggc tgttgccagg tttgtgcaat attttctcta aacgttgtca tccactcatc
    52441 agcgttagca aaaactttac caacactgat taactgaata aaaacaaaga aaaatatagc
    52501 gacatattta ctcacgttca ttggtgacca ttaaacattt atcaaaaatc tacta
//
LOCUS       DAZKHK010000014        50521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKHK010000014 DAZKHK010000000
VERSION     DAZKHK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.014"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..103)
                     /locus_tag="ACHOS5_000892"
     tRNA            complement(27..103)
                     /locus_tag="ACHOS5_000892"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(67..69),aa:Arg,seq:acg)
     gene            complement(168..244)
                     /locus_tag="ACHOS5_000893"
     tRNA            complement(168..244)
                     /locus_tag="ACHOS5_000893"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Arg,seq:acg)
     gene            complement(248..340)
                     /locus_tag="ACHOS5_000894"
     tRNA            complement(248..340)
                     /locus_tag="ACHOS5_000894"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(304..306),aa:Ser,seq:gct)
     gene            complement(656..841)
                     /gene="csrA"
                     /locus_tag="ACHOS5_000895"
     CDS             complement(656..841)
                     /gene="csrA"
                     /locus_tag="ACHOS5_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP9775759.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1076..3706)
                     /gene="alaS"
                     /locus_tag="ACHOS5_000896"
     CDS             complement(1076..3706)
                     /gene="alaS"
                     /locus_tag="ACHOS5_000896"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9775760.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3835..4335)
                     /gene="recX"
                     /locus_tag="ACHOS5_000897"
     CDS             complement(3835..4335)
                     /gene="recX"
                     /locus_tag="ACHOS5_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9775761.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4404..5465)
                     /gene="recA"
                     /locus_tag="ACHOS5_000898"
     CDS             complement(4404..5465)
                     /gene="recA"
                     /locus_tag="ACHOS5_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9775762.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5545..6042)
                     /gene="pncC"
                     /locus_tag="ACHOS5_000899"
     CDS             complement(5545..6042)
                     /gene="pncC"
                     /locus_tag="ACHOS5_000899"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP9775763.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6187..7272)
                     /gene="mltB"
                     /locus_tag="ACHOS5_000900"
     CDS             complement(6187..7272)
                     /gene="mltB"
                     /locus_tag="ACHOS5_000900"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP9775764.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRLQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7529..8092
                     /gene="srlA"
                     /locus_tag="ACHOS5_000901"
                     /gene_synonym="gutA"
     CDS             7529..8092
                     /gene="srlA"
                     /locus_tag="ACHOS5_000901"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9775765.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            8089..9048
                     /gene="srlE"
                     /locus_tag="ACHOS5_000902"
                     /gene_synonym="gutE"
     CDS             8089..9048
                     /gene="srlE"
                     /locus_tag="ACHOS5_000902"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9775766.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9059..9430
                     /gene="srlB"
                     /locus_tag="ACHOS5_000903"
                     /gene_synonym="gutB"
     CDS             9059..9430
                     /gene="srlB"
                     /locus_tag="ACHOS5_000903"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9775767.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTIHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9434..10213
                     /gene="srlD"
                     /locus_tag="ACHOS5_000904"
     CDS             9434..10213
                     /gene="srlD"
                     /locus_tag="ACHOS5_000904"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP9775768.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10319..10678
                     /gene="gutM"
                     /locus_tag="ACHOS5_000905"
     CDS             10319..10678
                     /gene="gutM"
                     /locus_tag="ACHOS5_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9775769.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHVGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10745..11518
                     /gene="srlR"
                     /locus_tag="ACHOS5_000906"
                     /gene_synonym="gutR"
     CDS             10745..11518
                     /gene="srlR"
                     /locus_tag="ACHOS5_000906"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP9775770.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11511..12476
                     /gene="gutQ"
                     /locus_tag="ACHOS5_000907"
     CDS             11511..12476
                     /gene="gutQ"
                     /locus_tag="ACHOS5_000907"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP9775771.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12473..13987)
                     /gene="norR"
                     /locus_tag="ACHOS5_000908"
     CDS             complement(12473..13987)
                     /gene="norR"
                     /locus_tag="ACHOS5_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP9775772.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAVAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14174..15607
                     /gene="norV"
                     /locus_tag="ACHOS5_000909"
     CDS             14174..15607
                     /gene="norV"
                     /locus_tag="ACHOS5_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP9775773.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKKILGFNLPV
                     DMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIVYDTMSNNTRMMADAIAQGIAE
                     TDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLRFR
                     NKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREIAR
                     QWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGTPW
                     SEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15604..16737
                     /gene="norW"
                     /locus_tag="ACHOS5_000910"
     CDS             15604..16737
                     /gene="norW"
                     /locus_tag="ACHOS5_000910"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP9775774.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGQTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(16961..19213)
                     /gene="hypF"
                     /locus_tag="ACHOS5_000911"
     CDS             complement(16961..19213)
                     /gene="hypF"
                     /locus_tag="ACHOS5_000911"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP9775775.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACTLEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNCLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAA
                     RWLAGEVQNG"
     gene            complement(19366..19893)
                     /gene="hydN"
                     /locus_tag="ACHOS5_000912"
     CDS             complement(19366..19893)
                     /gene="hydN"
                     /locus_tag="ACHOS5_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP9775776.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20042..21052)
                     /gene="ascG"
                     /locus_tag="ACHOS5_000913"
     CDS             complement(20042..21052)
                     /gene="ascG"
                     /locus_tag="ACHOS5_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP9775777.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELITAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIAFNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SR"
     gene            21312..22769
                     /gene="ascF"
                     /locus_tag="ACHOS5_000914"
     CDS             21312..22769
                     /gene="ascF"
                     /locus_tag="ACHOS5_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP9775778.1"
                     /translation="MAKNYAALASSVITALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSLAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            22778..24202
                     /gene="ascB"
                     /locus_tag="ACHOS5_000915"
     CDS             22778..24202
                     /gene="ascB"
                     /locus_tag="ACHOS5_000915"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP9775779.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQMKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPSDDEILKNTVDFVSFSYYASRCASAEMNANNCSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24300..24719)
                     /locus_tag="ACHOS5_000916"
     CDS             complement(24300..24719)
                     /locus_tag="ACHOS5_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9775780.1"
                     /translation="MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24745..24930)
                     /locus_tag="ACHOS5_000917"
     CDS             complement(24745..24930)
                     /locus_tag="ACHOS5_000917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775781.1"
                     /translation="MNIKTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TLPYDAEQTWHFRKVSV"
     gene            complement(24955..25422)
                     /gene="hycI"
                     /locus_tag="ACHOS5_000918"
     CDS             complement(24955..25422)
                     /gene="hycI"
                     /locus_tag="ACHOS5_000918"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP9775782.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            complement(25415..25825)
                     /gene="hycH"
                     /locus_tag="ACHOS5_000919"
     CDS             complement(25415..25825)
                     /gene="hycH"
                     /locus_tag="ACHOS5_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP9775783.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25822..26589)
                     /gene="hycG"
                     /locus_tag="ACHOS5_000920"
     CDS             complement(25822..26589)
                     /gene="hycG"
                     /locus_tag="ACHOS5_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP9775784.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26589..27131)
                     /gene="hycF"
                     /locus_tag="ACHOS5_000921"
     CDS             complement(26589..27131)
                     /gene="hycF"
                     /locus_tag="ACHOS5_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP9775785.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFDLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(27141..28850)
                     /gene="hycE"
                     /locus_tag="ACHOS5_000922"
     CDS             complement(27141..28850)
                     /gene="hycE"
                     /locus_tag="ACHOS5_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP9775786.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28868..29791)
                     /gene="hycD"
                     /locus_tag="ACHOS5_000923"
     CDS             complement(28868..29791)
                     /gene="hycD"
                     /locus_tag="ACHOS5_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP9775787.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIIKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29794..31620)
                     /gene="hycC"
                     /locus_tag="ACHOS5_000924"
     CDS             complement(29794..31620)
                     /gene="hycC"
                     /locus_tag="ACHOS5_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP9775788.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSIVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31617..32228)
                     /gene="hycB"
                     /locus_tag="ACHOS5_000925"
     CDS             complement(31617..32228)
                     /gene="hycB"
                     /locus_tag="ACHOS5_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP9775789.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(32353..32814)
                     /gene="hycA"
                     /locus_tag="ACHOS5_000926"
     CDS             complement(32353..32814)
                     /gene="hycA"
                     /locus_tag="ACHOS5_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP9775790.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33026..33376
                     /gene="hypA"
                     /locus_tag="ACHOS5_000927"
     CDS             33026..33376
                     /gene="hypA"
                     /locus_tag="ACHOS5_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9775791.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPLCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            33380..34252
                     /gene="hypB"
                     /locus_tag="ACHOS5_000928"
     CDS             33380..34252
                     /gene="hypB"
                     /locus_tag="ACHOS5_000928"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP9775792.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            34243..34515
                     /gene="hypC"
                     /locus_tag="ACHOS5_000929"
     CDS             34243..34515
                     /gene="hypC"
                     /locus_tag="ACHOS5_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP9775793.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGNCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34515..35636
                     /gene="hypD"
                     /locus_tag="ACHOS5_000930"
     CDS             34515..35636
                     /gene="hypD"
                     /locus_tag="ACHOS5_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP9775794.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35633..36643
                     /gene="hypE"
                     /locus_tag="ACHOS5_000931"
     CDS             35633..36643
                     /gene="hypE"
                     /locus_tag="ACHOS5_000931"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP9775795.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     LAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36717..38795
                     /gene="flhA"
                     /locus_tag="ACHOS5_000932"
     CDS             36717..38795
                     /gene="flhA"
                     /locus_tag="ACHOS5_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP9775796.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAAGLYPKFSHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            38840..39268
                     /locus_tag="ACHOS5_000933"
     CDS             38840..39268
                     /locus_tag="ACHOS5_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFP9775797.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(39307..39651)
                     /gene="ygbA"
                     /locus_tag="ACHOS5_000934"
     CDS             complement(39307..39651)
                     /gene="ygbA"
                     /locus_tag="ACHOS5_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP9775798.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            39937..42498
                     /gene="mutS"
                     /locus_tag="ACHOS5_000935"
     CDS             39937..42498
                     /gene="mutS"
                     /locus_tag="ACHOS5_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9775799.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42604..43260
                     /gene="pphB"
                     /locus_tag="ACHOS5_000936"
     CDS             42604..43260
                     /gene="pphB"
                     /locus_tag="ACHOS5_000936"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9775800.1"
                     /translation="MPSTRYQKINAHHYRHIWTVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTSAKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(43311..44078)
                     /gene="ygbI"
                     /locus_tag="ACHOS5_000937"
     CDS             complement(43311..44078)
                     /gene="ygbI"
                     /locus_tag="ACHOS5_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP9775801.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            44274..45182
                     /gene="ltnD"
                     /locus_tag="ACHOS5_000938"
     CDS             44274..45182
                     /gene="ltnD"
                     /locus_tag="ACHOS5_000938"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP9775802.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGGKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            45179..46441
                     /gene="otnK"
                     /locus_tag="ACHOS5_000939"
     CDS             45179..46441
                     /gene="otnK"
                     /locus_tag="ACHOS5_000939"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP9775803.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLAIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            46438..47076
                     /gene="ygbL"
                     /locus_tag="ACHOS5_000940"
     CDS             46438..47076
                     /gene="ygbL"
                     /locus_tag="ACHOS5_000940"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP9775804.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNIPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            47081..47857
                     /gene="ygbM"
                     /locus_tag="ACHOS5_000941"
     CDS             47081..47857
                     /gene="ygbM"
                     /locus_tag="ACHOS5_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP9775805.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            47946..49310
                     /gene="ygbN"
                     /locus_tag="ACHOS5_000942"
     CDS             47946..49310
                     /gene="ygbN"
                     /locus_tag="ACHOS5_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP9775806.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(49453..50496)
                     /locus_tag="ACHOS5_000943"
     CDS             complement(49453..50496)
                     /locus_tag="ACHOS5_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFP9775807.1"
                     /translation="MQGIKIYTILASSLISGSIFAQTEISTANSSTVNASYVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
BASE COUNT        12018 a        13632 c        13129 g        11742 t
ORIGIN      
        1 ccactaaagc catatcaagt aagagatggt gcatccggga ggattcgaac ctccgaccgc
       61 tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga aacttacttt
      121 actgcagatt ttttgatacc gccactaaag tcgtatcaag taagagatgg tgcatccggg
      181 aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc tgagctacgg
      241 atgcaaatgg cggtgaggcg gggattcgaa ccccggatgc agcttttgac cgcatactcc
      301 cttagcaggg gagcgccttc agcctctcgg ccacctcacc acacgcctct tacgagtgct
      361 tcgaagaact tgtttatcgc tcatcgtcgc tgcgtggcgc acatattact ttctgagact
      421 tataagtcaa acaatttttc ccacactttt atcgtttgca cacttcacgc tcaattagtc
      481 tgtaaaaacg tcaaaaagag tgttttatca acagaaaaac ggaggtctga cagatagcag
      541 taatgcaaaa aaatggagac ttaagttgaa tgaacgggag taaagcgaaa agacaatgga
      601 gtgaaagaga aattttgagg gtgcgtctca ccgataaaga tgagacgcgg aaagattagt
      661 aactggactg ctgggatttt tcagcctgga tacgctggta gatctcttca cggtgaacag
      721 aaacttcctt cggggcattt acgccaatac gtacctggtt gccctttacc cctaaaactg
      781 tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc agaatcagca
      841 ttctttgctc cttgaaagat taaaagagtc gggtctctct gtatcccggc attatccatc
      901 atataacgcc aaaaagtaag caatgacaaa cacattacat ctaagcagtc atggcattac
      961 attctgttaa acctaagttt agccgatata cacaacttca acctgacttt atcgttgtcg
     1021 atagcgttaa tgcgaatgcc gtgaagcgag tccacggcat tgcctgacgc ttatattatt
     1081 gcaatttcgc gctgacccag cctttcacac tggctaacgc tgcaggtaag gccgcagcat
     1141 ccgtaccacc ggcttgcgcc atgtcaggac gtccaccacc cttgccgccc acctgctgag
     1201 cgaccatacc aatcagttcc cctgctttca cacgatctgt gacgtcctta gatacgcctg
     1261 caatcagaga aaccttacct tcggctaccg ttgccagcac gataattgtc gaccccagct
     1321 gattttttaa atcgtcaacc atggtacgca acattttcgg ctcaacaccg ctaagctcgc
     1381 taaccaacag cttaacacca ttaacatcaa ttgccttact ggaaagattt gcgctctcct
     1441 gtgcggcagc ttgttcttta agctgttgta actctttttc cagctgacgc gtacgttcca
     1501 gtactgagcg cactttatca gccagattat tgctatcgcc tttcagcaga tgcgcgactt
     1561 cgcttaagcg atcgctgtct gcatgaacgg tggcgatagc gccttctccg gttaccgctt
     1621 cgatacgacg aacgcctgca gcagtacccg attcagagat gatgcggaac agaccaatat
     1681 caccagtgcg gctggcgtga gtaccgccac acagctcggt ggagaaatcg cccatgctca
     1741 gcacgcgtac gcgctcatca tacttctcgc caaacagcgc catcgcacct ttcgctttcg
     1801 ccgcttcgag atccatgatg ttggtttcga tcggcaagtt acggcgaatc tgtgcgttca
     1861 ccaggtcttc gaccgcacga atctcttctg gtttcatcgc ttcgttgtgt gagaagtcga
     1921 agcgcagcac tttgtcgtta accagtgaac ctttctgcga tacatgagta cccagaacct
     1981 ggcgcagcgc agcgtgcatc aggtgcgttg cggagtgatt cagacgaata cgggcgcgac
     2041 gagcctcatc aacatcagcc tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt
     2101 taccgatgtg accaatcgcc tggccgtatt tctgcgtatc ttccaccgcg aaggagaagt
     2161 tagcgccttt cagttcgcct ttatcgccaa cctgaccgcc ggattccgca tagaatggcg
     2221 tttgatccag cacgaccaca gcttcctggc ctgcattgat ggcatcaacc gatttaccat
     2281 caacaaacag cgcggtcact ttgccgttca gttccagatg gtcatagcct ttaaattcag
     2341 atgcgctgtc aacacggatc attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg
     2401 cacgacgacg ctgctcttcc attgctgcgt caaaaccagc ttcgtcaact ttgatgttgc
     2461 gctcacgaca aacatcagcc gtcaggtcaa ccgggaagcc gtaggtgtcg tacaggcgga
     2521 aagcagtttc accatccagc gtatcaccag aaagttttgc cagctcttca tccagcagcg
     2581 ccagaccgcg ctccagagta cgggcaaact gctcttcttc agtcttcagc acctgctcaa
     2641 cctgcgcctg ctggcgtttc aggtcttcac ctgcagagcc cataacgtcg atcagcggac
     2701 caaccagttt gtagaagaag gtttctttcg cgccgagcat attaccgtga cgcactgcgc
     2761 gacgaatgat acgacgcagt acataaccac ggttttcatt ggacggcatt acgccatccg
     2821 cgatcaggaa tgcacaagaa cgaatgtggt cagcgattac gcgcagcgat ttattgctca
     2881 gatcggtcgc gccagtgact ttcgctaccg cctggatcag cgtgcggaac aggtcgatgt
     2941 catagttaga gttaacgtgt tgcagcaccg cagcaatacg ctccagaccc ataccggtat
     3001 ctacagacgg tttcggcagc ggttccatcg tgccatcggc ctggcggttg aactgcatga
     3061 agacgatgtt ccagatctca atgtagcggt cgccgtcttc ttccggactt cccggagggc
     3121 cgccccaaat gtggtcgccg tgatcgtaga agatttcggt gcacgggccg cacggaccag
     3181 tgtcacccat ctgccagaag ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc
     3241 gaataatacg ttcgcgcggg atccctactt ctttttccca gatttcgtag gcttcgtcgt
     3301 cgctttcata gacggtaacc cacagacgct ctttcggcag ggcaaaccat ttttcgctgg
     3361 tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt
     3421 tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta accgacgttt tccaggtcgt
     3481 tgtgtttacc acccgcacgc acgcagcgtt gggaagtggt agcgcgggaa taattacgct
     3541 tgtcgagccc aaggaacaca tccttgaact ggttcatccc ggcgttggta aacaacaaag
     3601 ttgggtcgtt atggggtacc agggagctgc tggcaactac ctgatgtccc ttactatgga
     3661 aaaagtcgag aaacgcctga cggatctcag cggtgctctt gctcataatt atcctgaaat
     3721 caagctaacg aaatatcgct accagccgtt gcgtgtcgtt aaccgcccgg ctggtaactg
     3781 aaaagtggga ataagataag ttttcttgac tgggaagtaa aatcccgtat gcgttcagtc
     3841 ggcaaaattt cgccaaatat cctggatatc ttccatcaga tagccacgat agagcagaaa
     3901 acgctggatc ttaacttttt ctgaaaagac agttggcaaa ggttcgccat attttcgcgt
     3961 cgcctgatcg cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt
     4021 cgcttcgcgg gaaatacctt tctgattcag ttcctgacga atacgcgcag gtccataacc
     4081 tttgcggcta cggctggcga taaagcgcgc aacaaatcgg ctgtcatcaa gatagccatg
     4141 ttcatggcac caggcaataa cgcgctcgta atcttctgcc gtggcatcaa tctcttccgg
     4201 gccatttttg cccataatcg gtgccgcgag tttacgtcgc agttcttgct cactgtgatc
     4261 gcgcaccgcc agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga
     4321 tgttgattct gtcatggcat atccttacaa cttaaaaaag caaaagggcc gcagatgcga
     4381 cccttgtgta tcaaacaaga cgattaaaaa tcttcgttag tttctgctac gccttcgctg
     4441 tcatctacag agaaatccgg cgttgagttc gggttgctca gcagcaactc acgtactttc
     4501 ttctcgatct ctttcgcggt ttccgggtta tctttcagcc aggcagtcgc attcgcttta
     4561 ccctgaccga tcttctcacc tttgtagctg taccacgcgc ctgctttctc gataagcttc
     4621 tctttcacgc ccaggtcaac cagctcgccg tagaagttga taccttcgcc gtagaggatc
     4681 tggaattcag cctgtttaaa cggcgcagcg attttgttct tcaccacttt cacgcgggtt
     4741 tcgctaccca ccacgttttc gccctctttc accgcgccga tacgacggat gtcgagacga
     4801 acagaggcgt agaatttcag cgcgttacca ccggtagtgg tttccgggtt accgaacatc
     4861 acaccaattt tcatacggat ctggttgatg aagatcagca gcgtgttgga ctgcttcagg
     4921 ttacccgcca gcttacgcat cgcctggctc atcatacgtg ccgcaaggcc catgtgagag
     4981 tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg
     5041 ataacgtcta ctgcgccaga acgtgccagg gcatcacaga tttccagtgc ctgctcgccg
     5101 gtatccggct gggagcacag caggttgtca atatcgacgc ccagtttacg tgcgtagatt
     5161 gggtccagcg cgtgttcagc atcgataaac gcacaggttt taccttcgcg ctgcgctgcg
     5221 gcgatcacct gcaacgtcag cgtggtttta ccggaagatt ctggtccgta gatttcgacg
     5281 atacggccca tcggcagacc acctgcccca agcgcgatat ccagtgaaag cgaaccggta
     5341 gagatggttt ccacatccat ggaacggtct tcacccaggc gcatgatgga gcctttacca
     5401 aattgtttct caatctggcc cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata
     5461 gccattttta ctcctgtcat gccgggtaat accggatagt caatatgttc tgttgaagca
     5521 attatactgt atgctcatac agtatcaagt gttttgtaga aattgttgcc acaaggtctg
     5581 caatgcatac gcagtagcct gacgacgcac cgcatcacgg tcaccgctga agcattcccg
     5641 ccgggtaatg ccttcaccac gggcagtggc aaaagcaaac cagacggtgc cgacaggttt
     5701 ctcttcactg ccgccatccg gcccggcgat accactaata gacacggcgt aatcagcacg
     5761 agccgctttc agtgcgccta tcgccatttc caccacaaca ggttcgctca ccgcgccatg
     5821 ctgcgccagc gtctcttcgc gtacaccgat catttgcgct tttgcttcgt tactataggt
     5881 gacaaatccg cgttcaaacc aggcggaact gccggcaata tcggtaatca ctttcgctac
     5941 ccaaccgccg gtacaagact cagcagttgt tacggttgcg ccacgggctt tcagcgcctg
     6001 cccaacctgt tcgcttaact gcatcagttc actgtcagtc atcacaacct ctgtcagtca
     6061 aaaatttatg ccggacaaga taacactttc gtcaggaaga tggtgttggc gttgcaattt
     6121 tacgaggagg attcagaaaa aagctgatta gccagaggga agcccacgcc cccctcttac
     6181 aaatagttac tgtacgcgcg ccagcgccac ggcttgtcct aactgccaga ccgccattgc
     6241 gtaatgggtg ctgtggttat aacgggtaat ggtgtagaag ttcggcagac cgtaccagta
     6301 ctggtagccg gtgccaacat ccagacgcag caggctggct tgttgatggt tgcccagcgg
     6361 ctgctgtggc gttaaacctg ccgcggcaag ctgcgaaatg ctgtacctgg ttttgaagcc
     6421 atttggcaag cctggcgcct gaccgtttgc cattaccgcg acctgatcgc ctttcaccca
     6481 gccgtgcgct ttgaaataat tcgccacgct accgatcgca tcaaccggat cccacaggtt
     6541 gatatgcccg tcgccgctga aatctaccgc atattgtttg taagacgacg gcataaactg
     6601 accgtagccc atcgccccgg caaaggaacc tttcagattg agcggatcgt cctgctcgtc
     6661 gcgcgccatc agcaggaagg tttccagctc gccagaaaaa tactccgcgc ggcgtgggta
     6721 gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt ttccccatca cgcgccccca
     6781 gcgggtttca acgccgataa tcccgacgat aatttccggc ggtacaccat acacctgcca
     6841 cgcgcgattc aacgcatctt catactgatt ccagaaaacc acaccgttct gcacgttgtc
     6901 cggcgtaata aattttttgc gataacgcag ccatgcgccg ttcggacctg atggcggttt
     6961 caccgatgtg gttggtgcct ggttatccat cagccgcagt accgaatcca gacgtttcgc
     7021 ctgggagaga atttcctgca actgcagacg atcgaaaccg tgtttgttca ccattttgtc
     7081 gatgaactgc tgggcattcg ggttattggc gaagtcaccg cccatctgca tcacattgtg
     7141 ctgcggctca agcaggaagc cgccagacgg cgttccggtg gtcgtttcag tctcagtagg
     7201 ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg
     7261 acgcttgaac atgaggggtc catttaacag attcaaccag gggcaagtat ggtaaagcat
     7321 cccgccccgc acaaggaagc ggtagtcact gcccgatacg gactttacat aactcaactc
     7381 attctcctca ctaccctctt tatataaact ttcaaaataa aatatttatc tttcattttg
     7441 cgatcaaaat aacactttta aatctttcaa tctgattaga ttagattgcc gtttggtaat
     7501 aaaacaataa atactgaagg agagaacaat gatagaaacc attactcatg gcgcagagtg
     7561 gtttatcggg ctgttccaga aaggcggaga agtgtttacc gggatggtaa ccggcattct
     7621 tccgctgtta attagcctgc tggttatcat gaacgcattg attaatttta tcggtcagca
     7681 tcgtattgaa cgatttgctc aacgttgcgc cggtaaccct gtttcccgtt atctgctgct
     7741 accgtgcatt ggtacgtttg tcttttgtaa cccgatgacc ttaagtcttg gtcgctttat
     7801 gccggaaaag tacaaaccca gctattacgc ggcggcctct tatagctgcc actcaatgaa
     7861 tggcctcttc ccccatatca accctggcga actgtttgtt tatctcggca ttgccagcgg
     7921 tctgacaaca ctgaatctgc cacttggccc actggcggtg agctatctgc tggttggtct
     7981 ggtcaccaac ttcttccgcg gctgggtaac cgatctgacc accgccattt tcgagaaaaa
     8041 gatgggcatt caacttgaac aaaaagttcg tctggcagga gcaacatcat gacacgcatt
     8101 cggatcgaaa aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc
     8161 aaaaaaatcg tctatatcac cgccggtacc cgacctgcga ttgttgacaa actggcacag
     8221 cttactggct ggcaagctat cgacggattt aaagaaggtg aacccgcgga ggcggaaatt
     8281 ggtgtcgcgg taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacggcgt
     8341 attcccacca ttaatatcca ctcgacgggc aagtccggcc cgctggcgca gtacattgtg
     8401 gaagatattt atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgaggcgaca
     8461 ccacaaccct cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc
     8521 gatggtttac tggcgaaggt gggaatgggt atgggttctg ccgttgccgt gctgttccag
     8581 tctggccgtg acaccatcga cactgtatta aaaaccattc tgccgtttat ggcgttcgtc
     8641 tcggcgctta tcggaatcat tatggcttct ggccttggtg actggattgc ccacggtctt
     8701 gctccgctgg cgagccatcc actgggtctg gtcatgctgg cgctcatctg ctccttcccg
     8761 ctgctttcac ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt
     8821 ggcgtgcaga ttggcctcgg caatattccg ccgcatctgg ctttacctgc actgtttgcc
     8881 atcaacgcgc aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagctcgt
     8941 caggacacgg ttcgcgtcgg tgtcccttct gttctggtga gccgcttttt aaccggcgcg
     9001 ccaactgtac tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat
     9061 gaccgttatt tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag
     9121 cgaccagatg ctcatcacct ttcgtgaagg cgcgcctgcg gatctcgaag agtattgctt
     9181 cattcattgc cacggcgagt tgaaaggtgc actccatccc ggtttgcaat tttctctcgg
     9241 gcagcatcgc tatccggtga ccgccgttgg cagcgtggcg gaagacaacc ttcgcgaact
     9301 gggtcatgtc accctgcgct tcgatggttt aagcgaagcg gaatttccgg gcactatcca
     9361 tgtggcaggc cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt
     9421 taaggagtaa aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc
     9481 gttcctgtgc cacggtctgg ctgccgaggg gtatcgcgtg gcggttgtag atattcagag
     9541 cgacaaagcc gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gaacagcgta
     9601 cggttttggt gctgacgcca ccagcgagca aagcgttctg gcgctttctc gtggggtaga
     9661 tgaaatcttt ggtcgcgtgg atttgctggt ctacagcgcc ggaatagcaa aagcagcctt
     9721 tatcagcgac ttccagctcg gcgattttga ccgttcgcta caggtgaatc tggtgggtta
     9781 tttcctgtgt gcccgtgaat tttcgcgttt gatgatccgc gacgggattc agggtcgcat
     9841 tattcagatc aactcgaaat ccggcaaagt gggcagcaaa cacaactctg gctacagcgc
     9901 cgcgaaattt ggtggcgtcg ggctgactca atcactggcg ctggatctgg cggagtacgg
     9961 cattacggtg cattcgctga tgctcggtaa cctgctgaaa tcgcctatgt tccagtcact
    10021 gttgccacaa tacgcgacca agctgggtat caaaccggag caagtcgagc agtattacat
    10081 cgataaagta ccgctcaaac gcggctgcga ttatcaggat gtgctgaata tgctgctgtt
    10141 ctacgccagt cctaaggcgt cgtactgcac tggtcagtcg atcaatgtca ccggcggtca
    10201 ggtgatgttc tgatcaacag cggagatctt ttaaggatct ccgcgcgact aatagaatgc
    10261 ctgatgcgct acgcttatca ggcctacagg acttccgcca ctacattacg gagaagttat
    10321 ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt gcgcaactgg ccttaggcgg
    10381 ttggcaaatt tctcgtttta accgtgcctt cgacacgcta tgccagcaag ggagggttgg
    10441 cgtgggccgt tccagcgggc gctttaaacc gcgggtcgtg gtcgctattg cgctggacga
    10501 tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg accgtcttcg cccgaccgca
    10561 aaaaattccc gcaattaccg gcatgcatgt gggtgattta cagcccgatg tgatctttcc
    10621 ccatgatcca ctatcacaga atgctctatc attggcgctt aaactgaaac gtggataatt
    10681 tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga aacctaaatc aggtaatcac
    10741 gcccatgaaa cctcgtcagc gtcaggccgc cattctggag tatctacaaa agcagggtaa
    10801 atgctcggtt gaagaattgg cgcaatactt tgacaccaca ggcacaacca ttcgcaaaga
    10861 tctggtcatt ctggaacatg ccggaaccgt cattcgtact tatggcggcg tggtgttgaa
    10921 caaagaggaa tccgatccgc ctatcgatca taaaacgctt atcaacaccc acaaaaaaga
    10981 gctgattgca gaagctgccg ttggttttat tcatgatggc gactcaataa ttcttgatgc
    11041 tggcagtacc gtgttgcaga tggtgcctct gctatcgcgc tttaataaca tcacggtgat
    11101 gaccaacagc ctgcacatcg tcaatgcgct atccgaactg gataacgaac aaactatcct
    11161 gatgccaggc ggaacatttc gcaaaaaatc ggcctcattt cacgggcaac tggcagagaa
    11221 tgccttcgag catttcacct tcgataaatt gtttatgggc accgacggca tcgatctcaa
    11281 tgcgggcgta accaccttta acgaggtcta taccgtcagt aaggcgatgt gtaatgccgc
    11341 gcgcgaagtg attttgatgg ctgactcatc gaagtttggt cgtaaaagcc ccaacgtggt
    11401 ttgtagtctt gaaagcgtcg ataagctgat taccgacgca ggtatcgatc cagcgtttcg
    11461 tcaggcgctg gaagaaaaag ggatcgacgt gatcataacc ggagagagca atgagtgaag
    11521 cactactgaa cgcgggacgt cagacgttaa tgctggagtt gcaggaagca agccatttac
    11581 cggaacgtct gggcgatgat ttcgttcgcg ccgccaatat catcctgcac tgcgaaggca
    11641 aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg taagaaaatc gccgcaacgc
    11701 ttgccagcac cggcactcct gccttttttg tccatccggc agaggcattg cacggcgacc
    11761 tggggatgat cgaaagccgc gatgtgatgc tgtttatctc ttattccggt ggcgcgaagg
    11821 aactggatct cattattccg cgtctggaag acaaatctat cgcgctgctg gcaatgaccg
    11881 gaaaaccgac gtcaccgctg ggcctggcgg caaaagcggt gctggatatc tccgtagaac
    11941 gcgaagcctg cccgatgcac cttgcgccga cttccagcac cgtgaatacc ctgatgatgg
    12001 gcgacgcgct ggcgatggcg gtcatgcagg cgcgtggttt taatgaagaa gattttgccc
    12061 gctcccaccc agccggggca ctgggcgctc gcttgctgaa caaagtgcat catcttatgc
    12121 gtcgtgacga tgccatcccg caggtggcgt taaccgccag cgtgatggac gcgatgctgg
    12181 aacttagccg caccggttta ggtctggtgg cagtgtgcga tgatcaacga ctggtgaaag
    12241 gcgtctttac cgacggtgat ttacgtcgct ggctggttgg cggcggcgca ctcaccacgc
    12301 cagtcaatga agcgatgacg gtcggcggca ccacgttgca atcgcaaagc cgtgccatcg
    12361 acgccaaaga aatcctgatg aaacgcaaaa tcaccgccgc accggtagtg gatgaaaacg
    12421 gcaaactcac cggcgcaatt aacctgcagg atttctatca ggccgggatt atttaatcct
    12481 tcagccccag acgtttcgcc agccgatgca ggttggcgac gtcggtttcc agcatccgcg
    12541 cacaggcggc ccagttgtga tgattttgcg ccagtgcctg acgaatggtt tcacgctgga
    12601 acgcttctgt cgcttcacgc agattttgct taacaacggg caccgccacc gcttctggcg
    12661 gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc aagaatcact tcatcgccgc
    12721 tgcgggtggc tctcgacaat actaccgccc gatgaatagc atgttccagt tcgcgcacgt
    12781 ttcccggaaa attgtagtgt tgcagtaaat ttcgcgctcc ggcacttaat accacgcggg
    12841 agagccccag ccgcaaacga cactgctcgc agaaataccc cgccagcaga atgacatcat
    12901 cgccccgctc acgcagcggc ggcactgaaa gtggaaacac gctcaggcgg tggaacaagt
    12961 cagcgcgaaa tcgccctgcc agtacctctt cgcgtaagtc acggttagtc gccgccagca
    13021 cgcgcacatc gacccgcaaa ctgcggtcat cgccaacgcg ttgaatatcg ccatactgca
    13081 acaccctcag cagcttggcc tgcaatgcca acgacaactc gccgatctca tcgagaaaca
    13141 gcgtgccgtt atccgccatt tcaaacttcc cgctgcggtt actgatagca ccagtaaacg
    13201 cccctttcac atgcccgaac aactcacttt ccgccacact ttccggcagt gcggcacagt
    13261 tgagatagac cagcggattc accgcccgtg gcgaggcttc atgaatcgct ttcgccacca
    13321 gctccttacc ggttcccgtc tcaccgctaa ttaaaacgtt gagatcggat gccgccacaa
    13381 tctcaatctc ttttttcaat tgcgtcatgc ctggcgacag accaatcatc tgcgtctgtt
    13441 tcaccgcttc aaacggcgcg gcatcgcctg gcagcatatt ctggctttcc agttgttcaa
    13501 tcagcaatgc attgcttaac gctcccgccg ccagtgcggc aatcagccgt agctcttcgt
    13561 cgctgaaaac atcgaactga tcgggctgca tcccgtcgag cgtcaaggca ccgatcaggt
    13621 tttgcccggc aaacaacggc aaaccaacgc aggcgtgaac cttcagactc tcctgccctg
    13681 gaatcaaacc gtcataagga tcgggcaatt cgctgtctgc gggaaagcgc accacgtccc
    13741 cggcgcgggc aatcgcttcc agccgtggat gaccttccag cgcaaagcgt ctaccgagta
    13801 catccttcgc cagcccgtcg atggcaagcg gaataaactg ccgcgagtcg taacgtagca
    13861 acgcagacgc atcgcactcc agcacctgac gcagcgtggt gatcaggcgc tgaaaacgat
    13921 cctgatgacc aatcccacgc tgcaattcga tggcgatatt cgccagcaca tcaacggaaa
    13981 aactcatctt tgcctcactg tcaatttgac tatagatatt gtcatatcga ccattttatt
    14041 gatagtcatt ttgactactc attaatgagc acaattttat ttatagagta aaaacaatta
    14101 gataaaaaac tggcacgcaa tctgcaatta gcaagacatc tttttagaac acgctgaata
    14161 aattgaggtt gctatgtcta ttgtggtgaa aaataacatt cattgggttg gtcaacgtga
    14221 ctgggaagtg cgtgattttc acggtacaga atataaaacg ctgcgcggca gcagctacaa
    14281 cagctacctc atccgcgaag aaaaaaacgt gctgatcgac accgtcgacc ataaattcag
    14341 ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg gcggatatcg attacatcgt
    14401 aattaaccat gctgaagagg accacgccgg ggcgctgacc gaactgatgg cacaaattcc
    14461 cgatacgccg atctactgta ctgccaacgc tatcgactcg ataaatggtc atcaccatca
    14521 tccggagtgg aattttaatg tagtgaaaac tggcgacacg ctggatatcg gcaacggcaa
    14581 acaactcatt tttgtcgaaa cgccaatgct gcactggccg gacagcatga tgacttacct
    14641 gacaggcgac gcggtgctgt tcagtaacga tgctttcggt cagcactact gcgacgagca
    14701 tctgttcaac gatgaagtgg atcagactga gcttttcgag cagtgccagc gttactacgc
    14761 caatatcctg acgccgttca gccgcctggt aacgccgaaa aagatcctgg gctttaactt
    14821 gccagtcgat atgatagcga cctctcacgg cgtggtatgg cgcgataacc cgacgcaaat
    14881 tgtcgagctg tacctgaaat gggcggcgga ttatcaggaa gacagaatca ccattgtcta
    14941 cgacaccatg tcgaataaca cccgcatgat ggctgacgct atcgcccagg ggattgcgga
    15001 aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat
    15061 cctgaccaat gtcttccgct caaaaggcgt actggtcggc acttctacga tgaataatgt
    15121 gatgatgccg aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa
    15181 acgcgccagc gctttcggct ctcacggctg gagcggcggt gcggtggatc gtctttccac
    15241 gcgcctgcag gatgcgggtt tcgaaatgtc gcttagcctg aaagccaaat ggcgaccaga
    15301 ccaggacgct ctggagttat gtcgtgaaca cggtcgcgaa atcgcccgtc agtgggctct
    15361 cgcgccgctg ccgcagagca cggtgaatac ggtggttaaa gaagaaacct ctgccgccac
    15421 gacggctgac ctcggcccac ggatgcaatg cagcgtctgc cagtggattt acgatccggc
    15481 aaaaggcgag ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa
    15541 cttcctgtgc ccggaatgct ctctcggcaa agacgtcttt gacgaactgg catcggaggc
    15601 aaaatgagca acggcattgt gatcattggt tcgggcttcg ccgcccgcca actggtgaaa
    15661 aatattcgca aacaggacgc cagtattcca ttaaccctga ttgccgccga cagcatggat
    15721 gagtacaaca aacctgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt
    15781 acccgccaga cggcaggtga atttgccgag cagtttaatc tgcgcctgtt tccgcacacc
    15841 tgggtaacgg atatcgatgc cgaagcccat gtggtgaaaa gtcagaataa tcagtggcaa
    15901 tacgacaagt tagtgctggc aaccggtgcc agcgcctttg tcccgccagt gcccgggcgt
    15961 gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg
    16021 gatgcccgac gcgtgttgat tgtcggcggt ggtttgattg gtagtgaact ggcgatggat
    16081 ttttgtcggg caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtcg
    16141 ttaatgccac cggaagtcag cagccgcttg cagcatcggt tgacggagat gggcgttcat
    16201 ctgctgttga aatctcagtt gcagggacta gaaaaaacag attctggcat tctggcaacg
    16261 ctggaccgcc agcgctgcat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg
    16321 gaaaccgccc tggcacgacg cgccgggcag acgattaatc gcggcgtttg cgtcgatagt
    16381 tatctgcaaa ccagtaatgc cgatatttat gcgctgggcg attgcgcgga aattaacggt
    16441 caggtattgc cgttcctcca gccgattcaa cttagcgcga tggtgctggc aaaaaatctt
    16501 ctcggcaata acacgccgct gaaactcccg gcgatgctgg tgaaaatcaa aacgccggaa
    16561 ttaccgctgc atctggcagg cgaaacccag cgtcaggatt tacgctggca aattaatacc
    16621 gaacgccagg gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg
    16681 gtcagtgagg atcggatgaa agaggcattt ggattgttga aaacgttgtc gatgtaggcg
    16741 agctacagtg ccccaaatgt cggatgcggc gctgacgcgt cttatccgac ctacagggaa
    16801 cgcatgtgtt gatcacataa tacgtttatg tcgcatcctg cactgtccca aaatgtcgga
    16861 tgcgacgctg gcgcgtctta tccgacctgc ggggatgaat atgtaggccg gataaggcgt
    16921 tcacgccgca tccggcaatg gtgtccaaat gcagcacgtt ttatccgttc tggacttccc
    16981 ctgccaacca acgcgccgca acaataaccc cctgccccag agacaacccg ccatcgcccg
    17041 caggtaaact ttgtgggaag agcaacgtaa aatcagcaag ataatgcgcc agacgtgcac
    17101 gcagcaaaca gttatgaata actccgccgc taaataccag cgtagtgata ccccgcatcg
    17161 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17221 cgcgttgatt aaccggagcc tgccagctca gccactgctg ccagaaagtg gcgagatcca
    17281 gttgattgtc caccagcggc atcgtcaccg gatgcgtcac gccgtggcac gaagccgcca
    17341 gcgcctccag cgtacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17401 gcgccgccgc tacggcatcg aacaaacgtc cacacgacga tgccagcggc gcgttaattc
    17461 cacgctcaat agcccgcgcc agcacgctcc agttttgctg ttgcacactt gccgtttcag
    17521 agtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17581 gcttcgcagc caaatcgccc cccggaagcg ccactgcggg caagccgccc aggtgctggc
    17641 attcgcgata gttcacccgc aagcactcgc cgccccacaa agcgccgttc tcccccatac
    17701 caataccgtc gagcgtcaac gcaatgacat ctccgccatc cagaggccag aggtgttctg
    17761 ccagacacgc cgctgcgtgg gcatgatgat gcagcaccgt ttgcgtcggc agattcatct
    17821 cacgcgccca ctggctggag acatagcccg gatgcgcgtc atgcacaacg tattgcggag
    17881 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17941 cgtcacttaa atcgcccaga tgctgactca acaccgcttg ttcgccgcgc accaggcaga
    18001 aggtattttt cagatccgcg ccgagacaca gcacaggcgg aacattttta aagcccggag
    18061 gcaaagccag cgcatccggt acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    18121 gcaccaccga atcatccatc cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg
    18181 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agccggtggt ttaccgctca
    18241 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18301 ggttcgcagg caacattacc ccgacttcgt taaggtcagg ggcgatatca tcacaaagct
    18361 caggtacata ttttttatcc accagcacaa tcggcgcggc gggcgtggta agcaactggc
    18421 gcgcagcgtc tggtaaaccg tcagccactg gcaacatgac cgccagcggt ttcgccgggc
    18481 gatgtttgcg cgcccgaagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18541 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18601 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgggggc
    18661 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcacagtact
    18721 ctttgtcaca agccggacac agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18781 gcatggcgcg aataatggtg aaacgcggac cacagtgagt acagttgata aacggataac
    18841 gccagcgtcg ttcgcctggg gtattcattt cggcaagaca agcagggcaa gtcgcggcat
    18901 cgggaacaat ttgcgtattc atgacgccac ccgcgctctg gcggatggtg aactcagtgg
    18961 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcgggc
    19021 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    19081 catcgccgtc attacagaca tcgccgtgaa gatttaattg ctgtgccagt tgccagacaa
    19141 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaagatg
    19201 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc cagccggaca
    19261 cgacagcgtc gcatccggca gtcacaggtc ggcgataccg cccgcttcgt attctacgaa
    19321 tatttccggg aattcctttg ataccagaac agttctgtaa gatttttaga acatcagcgc
    19381 cgtgcggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga cacaaatcag
    19441 cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc
    19501 gcatttattg gcttcggctt tgtcagcccg tacattcagc cctgcgccgc tgttgcgaat
    19561 caccggacgc accaccactt ccatcgcacc atacgggcaa gccacaacgc aggttttgca
    19621 accaatacaa cgttcctgca tgacatgaac aaacccttta tcacggctga tagcaccatt
    19681 cgggcagacg ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat
    19741 gttcacacct ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca
    19801 gtcctgattt tcctgatgag aaaccacgca cgctacttca caggtacggc aaccaataca
    19861 tttactcgcg tcagcaatga tgaaacggtt catcaaattc tccagcaata acagttaatg
    19921 cgccgataca ttcacaaatc atgccagttt ttaatttact gttatttaag gaaattaaat
    19981 tctgtactac aggaaaaacg atgtcatcga cactagtgac gatgacatgt gatgacaatg
    20041 tttatcgcga aagagcaatg agtgagccgc ggcggattag ttttccgctg aaggttttcg
    20101 gcggtgagaa atccccgcca tcgagcataa aaatcagccg tccaataatt tcctgaatca
    20161 tctcagtcac cggaattttt acgctggaga gcgccggaac gatgtagggg gcaatagcga
    20221 tatcatcgaa tccgataact gacacctgct ctggcaccgc tacgccgcgc tcgtgtaacg
    20281 ctttgatcgc acctatcgcc atatcgtcgt tactggcaac taacgcgcta aatttagccc
    20341 cacgttcgag caacgtttct accccttcgg caccgctggc gggcgtccat ttaccgttag
    20401 cgataagttt ttcattgaac gcaataccat gctgcgccag cgcgtcttta tatccggcaa
    20461 gacgttcaat gctggtgggg gaatccatcg agccggtaag gaaagcaatc tcctgatgtc
    20521 cggcggttat caactctgcc acggcgttaa agctggtctg tttatgatcg caccagacgc
    20581 tatggctgct gtttttgcgc aggcggcgat taagcaccat tatcggctga ctgtgtgcgt
    20641 caatgatgtc atcaatctca tccacgctta aaaagcgcgg gtaaatcatg atggcgtcgc
    20701 agcgcaaatc cagcagatac tgaatcgcct gacgctcttc ttcggcgcta tgtttaccat
    20761 ctgccaatag caactgtcgc cctttctctt ccgccattcg cgcggcatga aacagtaatt
    20821 cactaaaata aatgccatgg taaagcgtgt tggtcacgac cagccccagc gtctgagtac
    20881 tcttcgccga cagattgcgc gccagcaagt ttggacggta accgctctct tctaccgcct
    20941 gaaacacgcg atctttagtc tcctggctga cgtagccatt acctgaaagc acgcgggaaa
    21001 cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag catcgtcgtc atcattttca
    21061 tccctttaca cgcaatcaac gcagtgtact gcaccgtttg ccgattgtcc ttgcgcaatc
    21121 agcgggaaaa atattcaggt gaccggtttc acaaatataa aaaatgaaca attaactctc
    21181 ttgcttatta aatgacaact attcatgatt ttgtgaaacc ggtttcctaa ttccgtttca
    21241 gcatcggcat ttttccgtca cgtcgactga taacaactac atctacccta ctgataacag
    21301 gataaaatcc gatggccaaa aattatgcgg cgctggcaag ctcggtgata acggcactgg
    21361 gcggcgttga taacatctcg gcggtcacgc actgtatgac gcgtttgcgc tttgttatca
    21421 aagatgacca gcttatcgac agtccgacgt tgaaaaccat ctccggcgtg ctcggcgtgg
    21481 tacgtagtga caaccagtgt caggtgatta taggtaatac ggtttcgctg gcctttcagg
    21541 aagtcgtcag cctgctgccg ggagatatgc agcccgcaca gcccgtgggt aaacccaaac
    21601 ttacgctacg tcgcataggt gcggggatcc tcgatgcgct gatcggcacc atgtcaccgc
    21661 tgatcccggc gattatcggc ggatcgatgg tcaaactgct ggcgatgatc ctcgagatga
    21721 gcggcgtgct gacaaaagga tcgccgacct taaccattct gaatgtgatt ggtgacggtg
    21781 ctttcttctt cctgccgctg atggtcgctg cttctgccgc catcaaattt aaaaccaata
    21841 tgtcgctggc gattgccatt gcgggtgtgc tggtacatcc gagttttatt gagctgatgg
    21901 cgaaagcggc acagggtgaa catgttgaat ttgccctgat tccggtcacc gcggtgaaat
    21961 acacctacac agtgatcccg gcgctggtca tgacctggtg cctgtcatat atcgaacgct
    22021 gggtggacag cattacgcca gcggtgacga aaaacttcct caagccgatg ctgattgtgt
    22081 tgattgccgc accgctggca atcctgctga ttggcccgat tggtatctgg atcggtagcg
    22141 ccatttcggc gctggtttac accattcatg gttatctggg ctggctttca gtcgccatta
    22201 tgggcgcgct gtggcctctg ctggtaatga cagggatgca ccgcgtcttt acgccaacca
    22261 tcattcagac cattgccgaa accggcaaag aagggatggt catgccgtca gagatcggcg
    22321 ctaacctgtc gctgggcggt tcatcactgg cggtggcatg gaaaacgaaa aacccggaac
    22381 tgcgccagac ggcgctcgca gcggcggcat cagccattat ggcggggatt tccgaaccgg
    22441 cgttatacgg cgtggcgatc cgcctgaaac gtccgcttat cgccagtctt atcagcggtt
    22501 ttatttgcgg cgcggttgcc ggtatggcgg ggcttgccag ccactcaatg gcagcgccgg
    22561 ggctatttac cagcgtgcag ttcttcgatc cggcgaatcc aatgagcatc gtctgggtgt
    22621 tcgccgtcat ggcgctggcg gtcgtgctgt cgttcatcct cacactgttg ctcggctttg
    22681 aggatattcc tgttgaggaa gcgactgccg aggcgcgaaa gcatcagagc gtacaaccga
    22741 ccgtcgccaa agaagtaagt cttaattgag gatgaaaatg tcagtatttc cagaaggttt
    22801 tttatggggc ggcgcgcttg ccgccaacca gtctgaaggc gcgtaccgtg aaggcggcaa
    22861 agggctgacc accgtcgata tgatcccgca cggcgagcat cgaatggcgg tgaaactggg
    22921 actggaaaaa cgttttcagt tgcgcgatga cgagttttat cccagccatg aggcgacgga
    22981 tttttatcat cgttataaag aagatatcgc cctgatggca gagatgggat tcaaggtttt
    23041 ccgtacctca attgcctgga gccgcctctt cccgcagggc gatgaactga cgccaaacca
    23101 gcagggcatt gctttttacc gcgcggtatt tgaagagtgt aaaaagtacg gtatcgaacc
    23161 gctggtcacg ttatgccact tcgatgtgcc gatgcatctg gtaaccgaat atggctcctg
    23221 gcgtaaccgc aagctggtag agcttttcag ccgctacgcc cggacctgct ttgaagcatt
    23281 tgatggtctg gtgaaatact ggcttacctt caatgaaatc aacattatgt tgcatagccc
    23341 gttctccggc gcgggtctgg tgtttgaaga aggggaaaat caggatcaga tgaaatatca
    23401 ggctgcgcat caccagctgg ttgccagtgc gctagccacc aaaatcgccc atgaggttaa
    23461 cccgcaaaat caggtgggtt gtatgctggc gggcggtaac ttctaccctt acagctgcaa
    23521 gccggaagat gtctgggcgg cgctggagaa agatcgggaa aacctgtttt ttatcgatgt
    23581 gcaggcgcgg ggcgcgtatc cggcttactc tgcccgcgta ttccgcgaaa aaggggtaac
    23641 catcgacaaa gcaccgagcg atgatgagat cctgaaaaac accgtcgatt ttgtctcttt
    23701 cagctattac gcctcgcgct gcgcctcggc ggagatgaac gccaacaact gcagtgcggc
    23761 gaacgtggtg aaatcgctgc gtaacccgta tctgcaagtg agcgactggg gctggggtat
    23821 tgatccactc ggtctgcgta tcaccatgaa tatgatgtac gaccgttatc agaagccact
    23881 gtttctggtg gaaaacggcc tgggcgcaaa agatgaattt gctgccaatg gcgaaattaa
    23941 cgacgactat cgcatcagct atttacgcga acatatccgc gcaatgggcg aagcgattgc
    24001 agacggcatt ccgctgatgg gctacaccac atggggatgt attgatttag tttccgcctc
    24061 tacgggtgaa atgagcaaac gctacggttt tgtctacgta gaccgtgacg acgcaggcaa
    24121 cggcacgctg acgcgcacgc gtaagaaatc gttctggtgg tataaaaaag tgattgccag
    24181 taatggtgaa gatttagagt aagtaaccgt gccggatgcg gcgtgaacgc cttatccggc
    24241 ctacaaggcc cgataagcgt tgcgcatcgg gcaatctggg gtttggcatc agtcaaaaat
    24301 caaagaaacc attccggctt aacgccaaat cgggctgaaa gtgccttaat gtgggatatc
    24361 gtcagggatc gttgtccaga taaaatctga ctgaccaggg atttagagcc gatttcgttc
    24421 ttcaaatccg cataggtcag attatgttga tcaatcagcg tacgaagtaa cgccacgcca
    24481 accggcattg ctgcaacggc tttgtcgaac tctgcaaact tttcgttatt gttttcatat
    24541 tctgcaatgc ggctcgccag aaagtcgata agtggattgg tgtcatcttc ttcaatcaaa
    24601 tattcgacca gttccagtgc ttcacgataa tcatcttctg agtcactgcc tcccagaaag
    24661 gggactgcat tgaccagttc tcttgttgct ttaacagcgc gtacagcatt agcagtcatt
    24721 ctttgttctc tctgtagtat cgtgtcagac gctcactttc ctaaaatgcc aggtttgctc
    24781 ggcatcataa ggcagggtta agagaggttt cagcgcccgc ttttgcgcgt attcgctgtc
    24841 actcattggc agatattcgg taaagctggt ggcctgtgcg ggcagcacta agctcaataa
    24901 aaaagcgcac atccctgttt tgatgttcaa ttccctgaat tccctaatga agaattattc
    24961 ttccaccgct aactgcgcga agccgccgtt cccttcccag ccttccagtc gttgataaac
    25021 ggtttctacc gcgtctttaa tcggctgggt catcgggtag taaaagccga cgatatccgg
    25081 ctggatgccg aggaaaatca cttcgccaat atcttctttc aactggtcga taaggtaatt
    25141 caacggcatg ttatgggtag tcatcataaa catctcggcg atgtcagccg ggtcgatgat
    25201 gcggatctcg ccagggttta gccccatatc cgtggcgtcg acaataagca gccgtgtcgg
    25261 gcgcagttcg cggatagcga caatgtcgtt ttccggtgcg ctaccgccgt caatcactac
    25321 ccagttacct ttcggcgcgg cagcgcactt ttccgccagt agcgggcctg cgccatcatc
    25381 acccatcatg ctattgccaa cacagagtaa aacgtcagtc acgcagtctc ctcaccatca
    25441 aatagatggc gttttcctga tgaatatcat gcagcatact taacagcatt gtactccatt
    25501 ccttctgctg cgaagtttgc gctgcccggg ctttatcgaa tgcgttggcc agcatcggca
    25561 catggttaat gtcgatgacg atctcaccat atttcggcac gccttccatt ttgcggcggg
    25621 cttcgctgcc cgcctccaga gtggcaatcc atgccagata gtcatcccac gggcaggtaa
    25681 gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc aatcgccagg ctgtaataga
    25741 ccacctgctg cgcctcggcg ggcgtagcat cgttctcatc aatgaattta cggctcagtt
    25801 gactgaacac caccttttca ctcatcggat acgcgcctct tcaacaacat gattcagatg
    25861 gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc agccagcgtg caacctgttc
    25921 ttcgccctgc cctaattgcg tcaggaaatc atcggcaatc tggcgaccgt aacgataacc
    25981 cgccaggcgg cgtgcttcgc gatccacttt cacgcgcagc ggctgcacca tatcgccatg
    26041 cagaatctcc gccggttgtt catcctgctc acccggccca cgggcgtgga ttttctgctc
    26101 cagcaggccg agcgccatcg caaagccgta cagcgtggcg gcaggcgttg gcgggcagcc
    26161 agggatataa acatccactg gaacgatttt atccgtaccg ccccacacgc agtagagatc
    26221 gtggaagatc ccgccactgt taccgcaggc accgtaggag atacagattt tcgggtccgg
    26281 cgcggactgc cacgcacgca gcgcagggga tcgcattgca cgggtgaccg cgccggtaaa
    26341 cagtaaaata tccgcatgac gcggtgacgg aacgacttta atgccgaagc gttctgcatc
    26401 gaacagcggc gaaagcgtgg cgaaaatttc gatttcgcaa ccgttacagc cgccgcagtc
    26461 cacgcgataa acgtaggcag aacgtttgat ttttttcagt aacgacgcct tcatgctggc
    26521 gatggattca tccaccgtca tcgggaccgg aatgccgttg gcgtcacggg ggcctaataa
    26581 attgctcatc agatggcctc tttcatatgg cgagtcagtt caatacggtc ggacggcacc
    26641 aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc tttcgcggtg gttttccgcg
    26701 cggctgtcac cgttgtgctt aagcagcgca atggcgtagt cgatctcttt ctggacggcg
    26761 aaaggacgat tgcagacgcg gcagttgcac agcgcgaagc gggactgttg caggaaatct
    26821 tctttcttcc acaccgccag ttcgtactct tgcgacagtt tgatcgccgc tgtcgggcag
    26881 acttcttcgc agcgcccaca gaagatgcag cgcccaaggt caaactgcca cgccagctca
    26941 ccagtggcga ggtcggtttc aaccgttaag gcgtttgacg ggcaggcatt gacgcaagcc
    27001 gcacagccaa tgcactgctg cgggttctgc tctggcttac cacggaagtt tttatcaacc
    27061 gcaatcggct ccagcggata agacgaagtc gccgtgccgg ttttgatgac ttttttgata
    27121 aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt tacgctcaat gctgtagcgc
    27181 tcaagttctt tgtacggcac cactttgctc ttcttcttac gtacatcgac cacggtcatg
    27241 cggtcggtac aggagtagca agggtccagg ctaccgataa tcagcggcgc atcggaaacg
    27301 gtgttgccgc gcagcatata acgcagggtc ggccagttcg cgtaggtcgc cgcacggcag
    27361 cgccagcggt acagcttctg gttgtcgccg gtcatgctcc agtggatatc gtcgccgcgc
    27421 ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt aggtaaagcc ttccaccatc
    27481 agcgggccgc ccggcaggtt atccagaccg taatcgatca tattcagcgc ggtatagact
    27541 tcgttgatac gcactttcag acgggaaata acgtcgcagc cctgctcgct gtggacttcc
    27601 attggcagca ggccataacc aacaaacggg tgatcggcac gggtatcacg ggcgtgaccg
    27661 ctggcacgaa ccatcgggcc aacgttactg aagtcgcgag cgatttccgg gtccagacga
    27721 ccaatgccga cggtgcgttg ttccatgttc ggtgtgctca gcagcacatc caccagctcc
    27781 tgcacttcac gacgcatctg ttgtgccagt tggcgggtct ggatcatgtc gtctttcagc
    27841 agatcgcgac gaatcccgcc gatcaggttc aggccgtagg ttttacgtgc accggtaagg
    27901 atctctgcca ttttcatgga ggtttcacgc acgcggaaga actgcataaa gccggagtcg
    27961 aagccggtaa agtgacaggc caggccgaga ttgagcagat gcgagtgcag gcgttctacc
    28021 tccagcagaa tggcgcggat catctgcgcg cgttctggca cctgaatacc catcgcgttt
    28081 tccaccgacg tggtataggc ggtgctgtga gcaaagccgc agatcccgca cacacggtca
    28141 gagaggaagg tcacttcgtt gtaacccata cgggtttccg ccagtttttc catgccgcga
    28201 tggacgtaga acaggcggta gtcggcgtcg ataatgtttt caccatcgac aaacagacgg
    28261 aagtgacccg gttcgtcaga agtgacgtgc aggggaccaa tcggcacgac gttgtttttc
    28321 ttgtcgccca gttcgttgat gaactcgtag gtttcagcat cggtagtcgg tgccggacgc
    28381 tgacgataat ccatgctgtc tttacgcaga ggataaagtt catccggcca gtcatccggc
    28441 agcaccagac ggcgttcatc cggcagacca accggaatca aaccgtacat atcgcgcact
    28501 tcacgctcgc cccacaccgc cgccggaacg cgcggcgtca cggaaggata ctccggtttg
    28561 ttggcgtcaa cttcgacgcg aaccgttacc caacacttgg tgcccttctc catcgacagc
    28621 acgtaataaa cggcgtaatg accattcagt ttgcgttcgt cgttaccaaa cagcaccgac
    28681 agccaccccc cttgtttgta gtagagaaac tccaccactt ccggcaggta gttcaccttc
    28741 acggtgacgg ttagctgatc tttggtctgc caggcgtggt ccagcacgac gcccggaaat
    28801 gcctcattca gcgcggcgag ataatgttga cctaattttt cttcagacat gctcaaactc
    28861 tctttaatca cgccgccagc aaggagacga acgctaaaaa tgcaaagcca aacccagccc
    28921 aggtaatgcg cggagtaata tcaagacgca gacgggccat gctgttttcg aacagcgcga
    28981 taaccaggac gccgaccacc agtttgatga tggcaatcac cagcgccagc agcagcccac
    29041 cgacggtgaa ggtttccatt tgtccccacg gaataaacac cccgacgaac atctgcaaca
    29101 ccaccagctg tttcaggctg atgccccatt tcatgacgcc aaagccgctg ccgctatact
    29161 cagagagcgg accttcctgc aactcctgct cggcttccgc caggtcgaac ggcagtttgc
    29221 ccatttcgat aaaagtggcg aacgcacagg cacaaagcgc cagcaccagc gggatgctct
    29281 ggctcagtgg ccagtgataa acggtgtcgg tgatgttgct gatgttggtg gaaccggcaa
    29341 cctgtgcggc gacccacaga ccaagcagca acatcggttc aaccaacacg ccaagcatcg
    29401 cttcacggct cgcgccgata gcggtaaacg ggctaccagt atccagacca gaaatggcaa
    29461 agaagaaacg cgcgatggcg aagagataca gtaaggtgat caaatcgcca agttgcggca
    29521 gcggagaacc gacggtcacc accggcagcg cagtggcgat agtcagcatg acgcccacca
    29581 tcacatacgg cgtcaggcgg aacacccagc cggaggcatc cgggccaacg ctctggcggc
    29641 ccagcagttt gataatgtcg cgatactcct gcaacacgcc cggcccgcga cggttatgca
    29701 gacgggcacg cgccacacgg gtaataccgg agagcagcgg cgcaacggca aatagcacca
    29761 gcgcctgaat taacggatat aaaacactca ttctcaggct cctcgtgaaa caataatcac
    29821 caccagcacc gccagttcaa ccagcgccat ccggcggaac agcaacgcac tcccctcgca
    29881 ctgccagccc ggcaccagag acaccggatt cagccatttg cgtagtttca gcaccggcgc
    29941 aaacgcctgt ttcaccggca tggcaaaacc gtgagcggta atcaccattg atttttcatg
    30001 gtcgtagccg cacacccagg ccgcgccgcg ggaacgcgac ggcaaacgat cgcctttgca
    30061 aatcgccata atgatgaatg gcagcagcgg gcaggcaatc agcagcaacg tgatcattgg
    30121 ttgagaaacg gtggtgttag caggctccag cggcagaggt acagcagcag agagcatcgg
    30181 cagtagccac ggcgcagcaa caccgccaat tacgcagcaa atcgccagtg ccactatgct
    30241 tacgctcatc aggagcggcg cacaggtggc gttttcggcc tctttggtac gcggcgcgcc
    30301 aaggaaagtg acgccataaa ctttcgccat acacatcacc gccagcgcac cggtaattgc
    30361 cagccccacg gcgagcagcg gcccgagaag acgggcaaca aacgcgccac tattgctcag
    30421 tttgaagaag gattgataga taacccattc cccggcaaaa ccgttcagcg gcggcagcgc
    30481 agccattgcc atcagcccga ctaacatggc gatggagata accggcattt tcttgccaat
    30541 accaccgagt ttttcgatat cgcgatgacc ggtacggaac cagacgctcc ccgcaccaag
    30601 gaacagtacg ctcttgaaca ggctatggtt aagcagatgg tacagaccac caaccagacc
    30661 aagggcaatc agcgccggtt gttcgagcgc gatacccgtt acgccagcgc ccagccccag
    30721 caggataatg ccgatattct ccagggtgtg gtaagccagc agacgttgga tattatgttc
    30781 catcagcgca tacagaccgc cgacgaatgc ggtgatcatg ccgagtacca gcagtgcgat
    30841 cccccaccac agcggtgcat taccgcccag cagtgacaga gttaaaatgc ccagcaggcc
    30901 aattttcatg actaccgtgg aaaacagcgc ggcagctggc gcagaggcgt tagcatgtgc
    30961 ctgtggcacc cagccgtgca gcggaataat cccggccagc aggccaaagc caatcacacc
    31021 gagcagccag atatcggaac cgagcggcag ctgttgcata cgcatatcca gcaggcgcag
    31081 atccagcgta ccgtaacgct gccacagcag ccagcaggca atcgccagca gcagagtgcc
    31141 aagacgcccc agcgcaaacc acagtttgcc ctctttgctg ttgctggtga ggaacaccgc
    31201 acacagggcc atgatttcgg ccattaccac gaacatgccg aggttgctgg cgatgacggc
    31261 acagacggcg gcagccatca acatattgat ctgcaagccg ttgcatttca cctgcgcgtg
    31321 gcgatgccag tcaatgttgt agaggctgac aaacagaccg cacagaccga gcgtaatcag
    31381 ccaaatcgcg ttaagcggag agatttgcac atcgtagctt accagcgaca gcgcaccgct
    31441 cacgccaacc gcgccagtca gcacagtgaa gcccgcggct gccgtataca ggctacccac
    31501 cgcgccgcca attccggcta tccagccact tagcgctttt tgaaaagaaa agagaaatgc
    31561 cagaacagcg gcggcgacaa accacgccac gccgctattg atcagggaaa ttgcgctcat
    31621 ttagcctctc cactttgagc ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaaa
    31681 gtcagctcac gcttacgttt gctgacgcgg gcgatatcgg tgttatccac cagatgcagg
    31741 gctttagtcg ggcacatccg cacgcaggcc ggaccttgtt catcaaaact acaaaggtca
    31801 catttgacgg cgatcgcgcg aatacctggc acccagtcaa gcaatgtgct gacacgcgcc
    31861 ggagcaggcg gtgccggtgg cgctttcggg gtattggcgt ttgccggaat atccagcgga
    31921 cggctgccgg aaaattcaat tgcgccaaac gggcaggcga tgccgcacag tttgcagctt
    31981 acgcacaggc tttcattcag ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg
    32041 cagaccaccg cgcagggtgc atcttcacag tgatgacaga gctgcggcgc agattctttt
    32101 tcatttagca tcactcgcag gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct
    32161 gaacaggcgg cctcacaggt gtggcagccg atacagagcg tggagtcagc aattacaaaa
    32221 cgattcacca ggcattcctc aggtgattgt catttttgac gaaaacatgc cgatgaaatg
    32281 tcattttcga cactcatcga cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg
    32341 caaagcctca gctcatgctg ccgggctttg tccctttacc agttggctta aattcaccgg
    32401 cacattgttt tcaatggcgg tatataagct gtcgtaaacg ccagcgattt tttcgagata
    32461 gtgttccaga acctgttcgc gatcgcggat gttgaccttg ccatcaatca tgccgtcaat
    32521 gctcagttgc gcctgcgcaa tccgctgttt gtcttcgcca tcttttgttt cgacgtaata
    32581 ctcgatggtg tggctgttaa agccatccgc cagggtgacg taaatgcgaa aatgttcaaa
    32641 aaggacgaaa cagagttctt tgtccggcgc gcagctcatg gcgtgatgca ggcgcgcttt
    32701 cgataacgtg atcccctgaa ccagcgaatt gcagtagatg tgccactggt cctgtaggcg
    32761 acgatgccgc tgtgcgatgt aatcggcttt ctcgcttatt tcccaaatag tcattgtcag
    32821 gttacccgtt taacagagat gccagcttta agcattttct gtgccaactt ttaattcatt
    32881 gttattaaag cgatttctaa gataaagatg aatttcgtcg ccgtgtcgac gtgtcatttc
    32941 gacatcatcg acattattca ccgcagggat aatcaacact ggcacaatta ttgcttgtag
    33001 ctggcaatag ttaatgggag gcgatatgca cgaaataacc ctctgccaac gggcactgga
    33061 attgatcgaa cagcaggccg caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa
    33121 aattggcgca ttttcttgtg tcgaaaccag ctctcttgcc ttttgttttg atctggtttg
    33181 ccgcggcagc gtggcggaag gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg
    33241 gtgtgaaaca tgccaacagt atgtgacgct actgacccag cgcgtccgcc gctgtccact
    33301 gtgtcatggt gacatgctac agattgtggc agacgacggt ttacagattc ggcggataga
    33361 aatagaccag gagtgagcga tgtgtacaac atgcggttgc ggtgaaggca acctctatat
    33421 tgagggtgat gaacataacc ctcattccgc gtttcgtagc gcgccatttg ccccggcggc
    33481 acgcccgaag atgaaaatca ccggcattaa agcgcctgaa tttaccccca gccagactga
    33541 agaaggcgac ctgcattacg gtcatggcga agcgggcact cacgcgccgg gcatgagcca
    33601 gcgtcggatg ctggaagtcg aaattgacgt gctggacaaa aataaccgtc tggctgaacg
    33661 caaccgcgcg cgctttgctg cccgcaagca actggtgctc aacctggttt ccagccctgg
    33721 ttccggtaaa accaccctgc tgacggaaac tttaatgcgc ctgaaagaca gcgttccgtg
    33781 cgcggttatt gaaggtgacc agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg
    33841 caccccagcg attcaggtga acaccggtaa aggctgccat cttgacgcac agatgattgc
    33901 cgacgccgcg ccgcgcctgc cactggacga taacggtatt ctgtttatcg aaaacgtcgg
    33961 taacctcgtt tgcccggcca gcttcgatct cggtgaaaaa cacaaagtgg cggtgctttc
    34021 cgttaccgaa ggtgaagaca aaccgctgaa atatccgcac atgtttgccg ccgcctcgct
    34081 gatgctgctc aacaaagttg acttgctgcc gtatctcaat ttcgacgttg agaagtgcat
    34141 cgcctgcgcc cgtgaagtca atccagaaat tgaaatcatc cttatttccg ccaccagcgg
    34201 cgaagggatg gaccagtggc tgaactggct ggagacacag cgatgtgcat aggcgttccc
    34261 ggccagatcc gcactattga cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc
    34321 gatgtcgatt taacgttagt cggcaactgc gatgaaaacg gtcagccacg cgtgggccag
    34381 tgggtactgg ttcacgttgg ctttgccatg agcgtaatta atgaagccga agcacgcgac
    34441 actctcgacg ccttacaaaa catgtttgac gttgagccag atgtcggcgc gctgttgtat
    34501 ggcgaggaaa aataatgcgt tttgttgatg aatatcgcgc gccggaacag gtaatgcagt
    34561 taattgagca tctgcgcgaa cgtgcttcac atctctctta caccgccgaa cgccctctac
    34621 ggattatgga agtgtgtggc ggtcataccc acgccatttt taaattcggc ctcgaccagt
    34681 tgctaccgga aaacgttgag tttatccacg gtccgggttg cccggtgtgc gtactgccaa
    34741 tgggtagaat cgacacctgc gtggagattg ccagccatcc ggaagtcatc ttctgtacct
    34801 ttggcgatgc catgcgcgta ccagggaaac aggggtcgct gttgcaggca aaagcacgcg
    34861 gtgccgatgt gcgcatcgtt tactcgccaa tggatgcgtt gaaactggcg caggagaatc
    34921 caacccgtaa agtggtattc ttcggcttag gttttgaaac caccatgccg accaccgcca
    34981 tcactctgca acaggcgaaa gcccgcgatg tgcagaattt ttacttcttc tgccagcaca
    35041 ttacgcttat cccgacgctg cgcagtttgc tggaacagcc agataacggt atcgacgcgt
    35101 tcctcgcgcc gggccacgtc agtatggtta tcggcactga tgcctataat tttatcgcca
    35161 gcgattttca tcgtccgctg gtggtcgctg gtttcgaacc ccttgatcta cttcaaggcg
    35221 tggtgatgct ggtggagcag aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc
    35281 gggtggtacc ggatgccggt aacctgctgg cgcaacaggc gattgccgat gtgttctgtg
    35341 tcaacggcga cagtgaatgg cgcggcttag gcgtgattga atcttctggc gtgcacctga
    35401 cgccggatta tcaacgattc gatgccgaaa cacatttccg cccggcaccg cagcaggtct
    35461 gcgatgaccc gcgtgcgcgt tgtggcgaag tcttgacggg caaatgtaag ccgcatcaat
    35521 gcccgctgtt tggtaacacc tgtaatcctc aaaccgcgtt tggtgcgctg atggtttcct
    35581 ccgaaggagc gtgcgccgcg tggtatcagt atcgtcagca ggagagtgaa gcgtgaataa
    35641 tatccaactc gcccacggta gcggcggcca ggcgatgcag caattaatca acagcctgtt
    35701 tatggaagcc tttgccaacc cgtggctggc agagcaggaa gatcaggccc gtcttgattt
    35761 ggcacagctg gtagcggaag gcgaccgtct ggcgttctcc accgacagtt acgtcattga
    35821 cccgctgttc ttccctggcg gtaatatcgg caagctggcg atttgcggca ccgcgaatga
    35881 cgttgcggtc agtggcgcta ttccgcgtta tctctcttgt ggctttatcc ttgaagaagg
    35941 attaccgatg gagacgctga aagccgttgt gaccagcatg gcagaaaccg cccgcgcggc
    36001 aggcattgcc atcgttactg gcgatactaa agtggtacag cgcggcgcgg cagataaact
    36061 gtttatcaac accgctggca tgggcgcaat tccggcgaat attcactggg gcgcacagac
    36121 gctaaccgca ggcgatgttc tgctggtgag tggaactctc ggcgaccacg gggcgactat
    36181 ccttaacctg cgtgagcaac tggggctgga tggcgaactg gtcagcgact gtgccgtact
    36241 aacgccgctt attcagacgc tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac
    36301 ccgtggtggt gtaaacgcgg tggtacatga gttcgcggca gcctgcggtt gtggtattga
    36361 aatttcagaa gcggcactgc cggttaaacc tgctgtgcgc ggcgtttgcg aactgctggg
    36421 actggacgcc cttaactttg ccaacgaagg caaactggtg ctcgccgttg aacgcaacgc
    36481 ggcagagcaa gtgctggcag cgttacattc ccatccactg ggaaaagacg cggcgctgat
    36541 tggtgaagtg gtggaacgta aaggtgttcg tcttgccggt ctatatggcg tgaaacgaac
    36601 cctcgattta ccacacgccg aaccgttacc gcgtatatgc taataaaatt ctaaatctcc
    36661 tatagttagt caatgacctt ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt
    36721 catatacacc gatgagtgat ctcggacaac aagggttgtt cgacatcact cggacactat
    36781 tgcagcagcc cgatctggcc tcgctgtgtg aggctctttc gcaactggta aagcgttctg
    36841 cgctcgccga caacgcggct attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt
    36901 acgcgtcgcg tgaaaaagac acccccatta aatatgaaga cgaaactgtt ctggcacacg
    36961 gtccggtacg cagcattttg tcgcgccctg atacgctgca ttgcagttac gaagaatttt
    37021 gtgaaacctg gccgcagctg gccgcagctg ggctataccc caaatttagt cactattgcc
    37081 tgatgccact ggcggcggaa gggcatattt ttggtggctg tgaatttatt cgttatgacg
    37141 atcgcccctg gagcgaaaaa gagttcaatc gtctgcaaac atttacgcag atcgtttctg
    37201 tcgtcaccga acaaatccag agccgcgtcg ttaacaatgt cgactatgag ttgttatgcc
    37261 gggaacgcga taacttccgc atcctggtcg ccatcaccaa cgcggtgctt tcccgcctgg
    37321 atatggacga actggtcagc gaagtcgcca aagaaatcca ttactatttc gacattgacg
    37381 atatcagcat tgtcttacgc agccaccgta aaaacaaact caacatctac tccactcact
    37441 atcttgataa acagcatccc gcccacgaac agagcgaagt cgatgaagcc ggaaccctca
    37501 ccgaacgcgt cttcaaaagt aaagagatgc tgttgattaa tctccacgag cgggacgatt
    37561 tagcccccta tgaacgcatg ttgttcgaca cctggggcaa ccagattcaa accttgtgcc
    37621 tgttaccgct gatgtctggc gacaccatgc tgggcgtgct gaaactggcg caatgcgaag
    37681 agaaagtgtt taccactacc aatctgaatt tactgcgcca gattgccgaa cgtgtggcaa
    37741 tcgctgtcga taacgccctc gcctatcagg aaatccatcg tctgaaagaa cggctggttg
    37801 atgaaaacct cgccctgacc gagcagctca acaatgttga tagtgaattt ggcgagatta
    37861 ttggccgcag cgaagccatg tacagcgtac ttaaacaagt tgaaatggtg gcgcaaagtg
    37921 acagtaccgt gctgatcctc ggtgaaactg gtacaggtaa agagctgatt gcccgcgcga
    37981 tccataatct cagtgggcgt aataatcgcc gcatggtcaa aatgaactgc gcggcgatgc
    38041 ctgccggatt gctggagagc gatctatttg gtcatgagcg tggggctttt accggtgcca
    38101 gcgctcagcg cattggtcgt tttgaactgg cggataaaag ctcactattc ctcgatgaag
    38161 tgggcgatat gccgctggag ttacagccga agttgctgcg tgtattacag gaacaggagt
    38221 ttgaacgtct tggcagcaac aaaatcattc agacggacgt acgtttaatt gccgcgacta
    38281 accgcgatct gaaaaaaatg gtcgccgacc gagagttccg tagcgatctc tattaccgcc
    38341 tgaacgtatt cccgatccac ctgccgccac tacgcgagcg tccggaagat attccgctgc
    38401 tggcgaaagc ctttaccttc aaaattgccc gtcgtctggg gcgcaatatc gacagcattc
    38461 ctgccgagac gctgcgcatc ctgagcaaca tggaatggcc gggcaacgtg cgtgagctgg
    38521 aaaacgtcat cgagcgcgcg gtattgctaa cacgcggcaa cgtgctgcag ctgtcattgc
    38581 cagatattgc tttaccagaa cctgaaacgc cgcctgcagc aacggttgtc gcccaggagg
    38641 gcgaagatga atatcagttg attgttcgcg tgctgaaaga aacaaacggc gtggttgccg
    38701 ggcctaaagg cgctgcgcaa cgtctggggc tgaaacgcac gactctgctg tcacggatga
    38761 agcgactggg aattgataaa tcggcattga tttaacagat gtggttatac tcgatcaaca
    38821 ttcaatgaaa ggaggcagta tggcagtttc agcaagaaat caattaaccg gaacagttag
    38881 cgcagtagca atgggcgcgg tgaatgacga agtagaactg acgctggcag gtggtgcaaa
    38941 gctggtggca attgtgactc acagcagcca gcaagcgctg ggtctggcga aagggaaaga
    39001 agcgattgcc ctgattaaag ccccgtgggt aacgctggca acggaagact gtggactgaa
    39061 attctctgct cgcaaccagt ttgctggcag cgtgtcgaca atcaccgaag gcgcagtgaa
    39121 cgccactgtc catatcaaaa cggatgcggg ttttgaaatt gtggcagtgg taacaaatga
    39181 aagtcaggat gaaatgaaac tcaccactgg ctcgcgcgtt attgcattga tcaaagcatc
    39241 agccatcctg attgccacca aagcgtaagc gccttatttg ccggagagta tggctctccg
    39301 gcagatttat ttctttggac gatatttttc tggcaactcc ggcaccggac gcttgtcatc
    39361 gatgagatga cgcacggtta agatcggatg acgccatagc attctcggcc cggcccaacg
    39421 cataatctgt ttcatctctt cacgctttgc aggctggtaa cagtgcaccg gacactgctt
    39481 acaggctggt ttctcttcac cgaacacaca tttatccagc cgcttttgcg cgtaagcaaa
    39541 caacgcctcg taatgctccg gctctgctga cgcctgcggg catttcgctt gataaagatc
    39601 gatcattttt ttaatcgtca gtttttcacg agagatacgc ttgccggaca tgctgcctcc
    39661 atactcttaa ggtgcattta tattacaact taattttaaa ggggactatg gctctgaagg
    39721 aggaggtttc ttcaaccgac gaatttggcg atcttgtttc atcgtcatca cgcaaaagct
    39781 gcaaagcagc atttttcgcg gaaccgacat caagaactca cctttcgcgt cttcccctga
    39841 aatgattaac tccggtatca tgtgcgcctt atgtgattac aacgaaaata aaaaccatca
    39901 cactacattt aacatcaggg agccggactt aaccccatga gtacaataga aaatttcgac
    39961 gcccatacgc ccatgatgca gcaatatctc aagctgaaag cccagcatcc cgagatcctg
    40021 ctgttttacc ggatgggcga cttttatgaa ctgttttatg acgacgcaaa acgggcgtcg
    40081 caactgctgg atatttcact gaccaaacgc ggtgcttcag cgggagagcc aatcccgatg
    40141 gcggggattc cctaccatgc ggtggaaaac tacctcgcca aactggtgaa ccagggcgaa
    40201 tcggttgcta tttgtgaaca gattggcgat ccggcgacca gcaaagggcc ggtcgagcgc
    40261 aaagttgtcc gtatcgttac gccgggcacc atcagcgatg aagcgctgtt gcaggaacgt
    40321 caggacaacc tgctggcggc tatctggcag gacagtaaag gtttcggcta cgcaacgctg
    40381 gatatcagtt ccggtcgttt tcgcctgagc gaaccggcgg accgcgaaac gatggcggca
    40441 gaactgcaac gtacgaatcc agcggaatta ctgtatgcgg aagatttcgc cgagatgtcg
    40501 ctgattgaag gtcgccgcgg cctgcgtcgt cgcccgctgt gggagtttga aatcgacacc
    40561 gctcgccagc aattgaacct gcaatttggc acccgagatc tggtcggttt tggtgtggag
    40621 aacgcaccac gcggactttg tgctgccggt tgtctgttgc agtatgcgaa agatacccaa
    40681 cgcacgaccc tgccgcatat tcgttctatc actatggaac gtcagcagga cagcatcatt
    40741 atggatgccg cgacgcgtcg taacctggaa attactcaga acctggccgg cggtgcggaa
    40801 aatacgctgg cttcagtgct cgactgcact gtaacgccga tgggtagtcg tatgctgaaa
    40861 cgctggctgc atatgccagt gcgcgatacc cgcgtgttgc ttgagcgcca gcaaactatt
    40921 ggcgcattgc aggatttcac cgccgagcta cagccggtac tgcgtcaggt cggcgacctg
    40981 gaacgtattc tggcgcgtct ggcgttgcgt accgctcgcc cacgcgatct ggcccgtatg
    41041 cgtcacgctt tccagcaact gccagagttg cgtgcgcagt tagaaaatgt cgatagtgca
    41101 ccggtacaag cgctgcgtga gaagatgggc gagtttgccg agctgcgcga tctgctggag
    41161 cgagcaatca tcgacacacc accggtgctg gtacgcgacg gtggtgttat cgcatcgggc
    41221 tataacgaag agctggatga gtggcgcgcg ctggctgacg gcgcgaccga ttatctggag
    41281 cgtctggaag tccgcgagcg tgaacgtacc ggcctggaca cgctgaaagt tggctttaat
    41341 gcggtgcacg gctactacat tcaaatcagc cgtgggcaaa gccatctggc accaatcaac
    41401 tacatgcgcc gccagacgct gaaaaacgcc gagcgttaca ttattccgga gctgaaagag
    41461 tacgaagata aagttctcac ctcaaaaggc aaagcactgg ctctggaaaa acaactttat
    41521 gaagagctgt tcgacctgct gttgccgcat ctggaagcgt tgcaacagag cgcgagcgcg
    41581 ctggcggaac tcgacgtgct ggtgaacctg gcggaacggg cctataccct gaactacacc
    41641 tgcccgacct ttattgataa acctggcatt cgcattaccg aaggtcgcca tccggtagtt
    41701 gaacaagtac tgaatgagcc gtttatcgct aacccgctga acctgtcgcc gcagcgtcgc
    41761 atgttgatca ttaccggtcc gaacatggga ggtaaaagta cctatatgcg ccagaccgcg
    41821 ttgattgcgc tgatggcgta tatcggcagc tacgtaccgg cgcaaaaagt cgagattggc
    41881 ccgattgacc gtatctttac ccgcgtaggt gccgcggatg atctggcttc cgggcgttca
    41941 acctttatgg tggagatgac cgaaactgcc aatattttac ataacgccac cgaatatagt
    42001 ctggtgttga tggatgagat cgggcgtgga acgtctactt acgatggtct gtcgctggcg
    42061 tgggcgtgtg cggaaaatct ggcaaataag atcaaagcgt tgacgctgtt tgctacccac
    42121 tatttcgagc tgacccagtt accggagaaa atggaaggcg tcgccaacgt gcatctcgat
    42181 gcactggagc acggcgacac cattgccttt atgcatagcg tgcaggatgg cgcggcgagc
    42241 aaaagctacg gcctggcggt tgcagctctg gcgggtgtgc caaaagaggt tattaagcgc
    42301 gcacggcaaa aactgcgtga gctggaaagc atttcgccga acgccgctgc tacgcaagtg
    42361 gatggtacac aaatgtcttt gctgtccgta ccggaagaaa cttcgcctgc ggtcgaggca
    42421 ctggaaaacc tcgacccgga ttcactgact ccgcgtcagg cgctggaatg gatttatcgc
    42481 ttgaagagtc tggtgtaata ataattcccg atagtctttt gctatcggga atattaacaa
    42541 caactgacga atcaaataaa aataccctgt ataataggaa agcttatttt acagggtaaa
    42601 accatgccat ctacacgcta ccaaaaaatc aacgcccatc actatcgcca tatatggacc
    42661 gttggtgata ttcatggtga ctatcaatta ttacaatccc gcttacatca actctctttt
    42721 tgccctgaaa ccgacttact tatttcggta ggcgataaca tcgatcgtgg gcccgagagt
    42781 cttaacgtgc tgcgcctgtt aaaccagccc tggtttacat cggctaaagg caaccacgaa
    42841 gcgatggcgc ttgatgcatt cgaaactggc gatggaaata tgtggcttgc cagcggtggt
    42901 gactggtttt tcgatttaaa tgattcagag caacaagagg caatagatct gttgctgaaa
    42961 ttccatcacc ttccacatat tattgaaatc actaacgaca ccataaaata tgtcatcgca
    43021 catgcagatt atccggggaa ggagtatcag tttggcaaag aaatagcgga gagcgaatta
    43081 ctctggcctg ttgatcgtgt gcagaaatca cttaatagcg aattacaaaa aataaacggt
    43141 gccgacttct ttatatttgg tcatatgatg tttgataaca ttcagacgtt cgctaaccag
    43201 atttatattg ataccggatc gccgaaaagc gggcggctgt cattttataa aataaagtag
    43261 tctcatgctt cttctgtgaa gcatgagtaa cccggcgtta ttgcaggtca ttattcattt
    43321 ttcgctacca gcaaagagag atccagcttc gccagcgcgc ggctggcact ctccggcaga
    43381 ccatcgtcag taatgatctg atcaaactcg cttaatggta acgccagcca tgtcgccacc
    43441 tgtccatatt tcgtcgcatc acagaccaaa actcgctggc ggctggcact ggcaatcgcc
    43501 cgtttcaccg tgactttatc ttctgctggc gtagagatcc cccgcacact ccatgacgat
    43561 gcagaaataa aagcctgatc aatcatcagg ctacgcagca tggtcgcagc ggcttcccca
    43621 acacaggaac ggttttcccg acacactgcg ccgccagtgt gaataattgt gcaattactg
    43681 ttgtcgagca agtagtccgc aataacgaag tcgttagtaa ccactgtgag tggctccatg
    43741 tgagtcagat gctgtgcaat cgctaacgtg gtcgttcccg catccagata gatacaacgt
    43801 cccggctgaa caagacttgc cgccagcttg ccaatagccg ctttttgcgt cattgccagc
    43861 gcagttttta cctgatgaga aggttcatgc gccacccgtc ccggagactg gacgcccccg
    43921 gacaccagca caacggctcc ctgctgctcc agtttttgta aatcccgacg aatggtcata
    43981 tgtgacacat tcattctgtc cgttagttca gcaatactaa caatgccttt ttcagctacc
    44041 atctcaagga tgatttggcg acgctctacg ggtatcaact tttgctcctt cctttgtcct
    44101 gctgacattc tacgctattt gcctgcgaaa cgtgcgcggc gcaactaacg cttagttcac
    44161 ataaaataac acacaatgtt aatttttgtg aatcagctca ccatatcgtt atctttccgc
    44221 ccttatattc acaacatcaa acaaaatatc acttaaatta acaaggagag cagatgaaaa
    44281 cgggatcgga gtttcatgtc ggtatcgttg gcttagggtc aatgggaatg ggagcagcac
    44341 tgtcatgtgt ccgcgcaggt ctttctacct ggggcgcaga cctgaacagc aatgcctgcg
    44401 ctacgttgaa agaggctggt gcttgcgggg tttctgataa cgccgcgacg tttgccgaaa
    44461 aactggacgc tctgctggtg ctggtggtca atgcgaccca ggttaaacag gtgctgtttg
    44521 gtggaaaagg cgttgcacaa catctgaaac ccggtacggc agtaatggtt tcttccacta
    44581 tcgccagtgc tgatgcgcaa gaaattgcta ccgctctggc tggattcggt ctggaaatgc
    44641 tggatgcgcc ggtttctggt ggtgcggtaa aagccgctaa cggtgaaatg actgtcatgg
    44701 cctccggtag cgatattgcc tttgaacgac tggcacccgt gctggaagcc gttgccggaa
    44761 aagtttatcg catcggtgca gaaccgggac tgggttcgac cgtaaaaatt atccaccagt
    44821 tgttagcggg cgtacatatt gctgccggag ccgaagcgat ggcgcttgca gcccgtgcgg
    44881 ggatcccgct ggatgtgatg tatgacgtcg tgaccaatgc cgccggaaat tcatggatgt
    44941 tcgaaaaccg gatgcgtcat gtggtggatg gcgattacac cccgcattca gccgtcgata
    45001 tttttgttaa ggatcttggt ctggttgccg ataccgccaa agccctgcac ttcccgctgc
    45061 cattggcctc aacagcattg aatatgttca ccagcgccag taacgcgggt tacgggaaag
    45121 aagacgatag cgcagttatc aagattttct ctggcatcac tctaccggga gcgaaatcat
    45181 gatcaagatt ggcgttatcg ccgatgattt taccggcgcg acggatatcg ccagttttct
    45241 ggtggaaaac ggtctaccaa cggtacaaat taacggtgtt ccaacaggta aaatgccgga
    45301 agcaatcgac gcactggtga tcagcctgaa aacgcgctcc tgtccggtgg ttgaagccac
    45361 acagcaatcg ctggcggctc tgagctggtt gcaacagcaa ggttgcaaac agatctattt
    45421 caaatactgc tctactttcg acagtacggc gaaagggaat atcggcccgg ttaccgatgc
    45481 attaatggat gctctcgaca cgccgtttac ggtcttctct ccggccctgc cagtcaacgg
    45541 acgtacggtt tatcaggggt atttgttcgt aatgaatcaa ctgctggccg aatccggaat
    45601 gcgccatcac ccggtaaatc ccatgaccga cagctatctt ccccgtctgg ttgaagcgca
    45661 atccacaggg cgctgcggcg tcgtttcagc acatgttttc gaacaaggtg tgaaagccgt
    45721 tcgtcaggag ctggctcgct tacagcaaga gggctatcgc tacgcggtgc ttgatgcgct
    45781 gaccgaacac catctggaaa ttcaggggga agccttgcgc gatgccccac tggtaacggg
    45841 cggttctggt ctggcgattg gcctggcccg gcagtgggcg caagaaaacg gtaaccaggc
    45901 tcgcgaagca gggcgtccgc tcgctgggcg cggcgtagtg ctctccggtt catgctctca
    45961 aatgaccaac cgccaggtgg cacattaccg tcaaattgca ccagcccgtg aggttgatgt
    46021 ggcacgctgc ctctcaaccg aaactctggc cgcttatgca cacgaactgg cagagtgggt
    46081 tctgggccag gaaagtgtac ttgctccact ggtttttgcc accgcaagca ctgacgcatt
    46141 ggcagcaatt caacagcaat acggtgcaca aaaagccagt caggcagtag aaacactgtt
    46201 ttctcaacta gcggcgcggt tagcagcgga aggcgtgaca cgctttattg tcgcaggcgg
    46261 tgagacctcc ggcgtagtca cacagagcct ggcaataaaa gggtttcata ttggcccaac
    46321 catttcccct ggcgtgccgt gggtaaacgc actggataag cctgtctcac tcgcccttaa
    46381 atctggcaac ttcggtgatg aagccttttt ttcacgagcc caaagagagt ttttatcatg
    46441 agcgatttcg caaaagtaga gcagtctttg cgagaggaga tgacgcggat tgccagttca
    46501 ttctttcagc gcggatatgc aaccggttcg gctggcaatc tgtcgctgct tttacctgac
    46561 gggaatttac tggcgacacc gacaggttca tgcctgggca atctcgatcc gcagcggctt
    46621 tccaaagtca ccgcggatgg cgaatggtta agtggtgaca aaccctcgaa agaggtgctc
    46681 tttcatctgg cgctgtatcg caacaatccg cgctgtaaag cggtggtgca tttgcacagc
    46741 acatggtcga cggcgctttc ctgcctgcaa gggctggaca gcagcaacgt tattcgtccg
    46801 ttcacaccat acgtggtgat gcggatggga aatatcccgc tggtgcctta ttaccgaccg
    46861 ggcgataaac gcatcgcaca ggatctggcg gaactggcag cagacaatca ggctttttta
    46921 ctggcaaatc atggcccagt ggtttgcggt gaaagcctgc aagaagccgc caacaatatg
    46981 gaagagctgg aggaaacggc aaagctgatt tttattctcg gtgaccgccc gatccgttat
    47041 ctgaccgcag gtgaaattgc ggaattaagg agttaatgca atgcctcgtt ttgcagctaa
    47101 tttatccatg atgttcaccg aagtgccttt tattgaacgc ttcgccgcag cgcgaaaagc
    47161 cgggttcgat gctgtggagt ttctgtttcc ctatgactac tccacccagc aaatccagca
    47221 gcaactggtg caaaaccatc tgacactggc gctgttcaat accgctcccg gagatattaa
    47281 tgcgggagaa tggggattat ccgcccttcc cggacgtgag cacgaagcac gcgcagacat
    47341 cgacctggca ctggaatatg cattggcgct caattgtgaa caagtccatg tgatggcagg
    47401 cgtcgtgccc gctggcgaag atgctgaacg gtaccgggca gtatttattg ataatctccg
    47461 ctacgctgcc gaccgctttg cgccacacgg caagcgaata ttggttgaag cactcagccc
    47521 cggcgttaag cctcattatc tcttctccag ccagtatcag gcactggcta ttgttgaaga
    47581 ggttgcgcga gataacgtat ttattcaact ggatactttc catgcgcaaa aagtagatgg
    47641 caacctgaca catttaatcc gcgactacgc cgggaaatat gcgcacgtac aaattgccgg
    47701 actacctgat cgacatgaac cggacgatgg agaaatcaac tatccgtggc tgttccgcct
    47761 gttcgatgaa gtgggatatc agggctggat cggttgtgaa tacaaacctc gtggcctcac
    47821 cgaagaaggg cttggctggt ttgacgcctg gcgctaattc gctcaatccc taattacaac
    47881 gtacccatac atccccccat ctacggatgg ggtaggttaa ctataacttt cagacagggt
    47941 ttcccatgtc cacaattaca ttgttatgca ttgcgttagc tggcgtaatc atgctgttgc
    48001 tactggtcat caaggcaaag gtacaaccat tcgttgctct gctcctcgtc agcctgttag
    48061 tcgcacttgc ggcgggtata ccggcgggcg aagtgggtaa agtgatgatc gccgggatgg
    48121 gcggtgtact tggttccgtc actattatta ttggtctggg cgctatgctg gggcgtatga
    48181 tcgaacactc tggtggtgca gagtcactgg ctaattattt cagtcgcaag ttaggtgaca
    48241 aacgaactat cgctgcgctg actctggcag cgttcttcct cggtattccc gtcttctttg
    48301 atgtcggctt tattattctt gcgccaatca tttacggttt tgccaaggtt gccaaaatat
    48361 caccactcaa atttggcctg cctgtcgctg ggatcatgct cactgttcac gtggcggtac
    48421 cgccgcatcc aggccctgtc gccgcagcgg ggttactcca cgcagacatc ggctggctaa
    48481 ccatcatcgg tattgcgatt tctattcccg tagggattgt tggctacttt gcagcgaaaa
    48541 taatcaataa gcgccaatat gcgatgtcgg tcgaggtact ggaacagatg caactggctc
    48601 cggccagtga ggaaggcgcg acaaaattaa gcgataaaat aaatccaccg ggcgtcgcgc
    48661 tggtcacctc gctaattgtt attcctatcg cgattatcat ggcgggtacg gtttctgcaa
    48721 cactgatgcc gccttcgcat cccctgcttg gtacgctaca gttgatcggc tcaccaatgg
    48781 tagctctaat gattgcgctg gtgctggcat tctggttatt ggctttacgt cgcggctgga
    48841 gcttacaaca caccagcgac attatgggct ctgcccttcc cactgcggcg gtagtgattt
    48901 tggttactgg tgctggaggg gtatttggta aagtgctggt ggaatcgggc gtcggtaaag
    48961 cccttgccaa catgttacaa atgattgacc tgcctctgtt accagccgca tttattattt
    49021 cactggcgct gcgtgcatcg caggggtcag caaccgtagc aatcctgaca accggcgggt
    49081 tactctcaga agcggtgatg ggattaaatc caattcagtg cgtattggtg acgctggcag
    49141 cctgctttgg tgggcttggt gcgtcacata ttaatgactc agggttctgg attgtgacca
    49201 aatatctggg gttgtcggta gcagacggtc tgaaaacctg gacagtgtta acgaccattc
    49261 tcggttttac cggattctta attacctggt gcgtatggct cgtcatctaa taatccagat
    49321 aagatatgat gtatcgttta agtgcctgat aagggtgtaa tgtatgccaa tattaacctc
    49381 ggtaatatct cgctaccgag gttaattttc actttgcaat ataattacaa tctcttgcaa
    49441 gtttatttta tcttagtaat aagaaccgac caatcttatt gccatttcga tattagcagg
    49501 catagatgcg tcgtagccat acatatggcc atatttcgga cgtaatactt cagttacttt
    49561 cttaataatt gcttcgcgct gggctcgcgc aaaatcagca tgattatttg ccgttttatt
    49621 cataaattta ccaaaacttt cttcttttct gactttaatt gtcgtatccc gaagatcatt
    49681 aagaaactgg cgttgaaatt taaaatcatc gtgtgaacca atattaccat gcccgccaat
    49741 aaaatatttc cagtcaagca tttcaacctg acgcaaatta ctgtcatggt aaaccaacgt
    49801 atccgatacg gcgaagccca tcatcggcag ttggtcagga ttcaggagat ccgggctgtg
    49861 agctactttt tcctgtttta gcaaccatac agaatggtcg tcggtatgtc ctgctttaac
    49921 aaatcggtgt aattcaatgg tctgcttttc aaatttaaac tggtcatctt ttttacttaa
    49981 aacctgagtg gcgacgggta agcgactgtt cataaattgc attttttcag cagtggcttt
    50041 actggcaata atacgcagat tcacgccttc tttcttcgcc tcatcgttcc aaaatggact
    50101 atcgccaatg tgatccacat ggtaatgaga ataaaccagc gctgttacgg gtaatggagt
    50161 cacatcacga attgcctgta ataaatattt accgcgaccc tccggagcat caaacagcaa
    50221 aacccctttg tctccgacat aaaacgtagt ggaataaaaa tagcgttgaa cataataagt
    50281 tctctcgcca attttttgta aaatataagg ctgagacata tttctttcaa aaaggttatt
    50341 gagtttatcg cttggcgata ttattttttc cgctgaaggt tcaacataac ttgcattaac
    50401 tgtagacgaa tttgcagtcg aaatttccgt ttgagcaaaa atactaccag atataagaga
    50461 agaggctaga attgtatata ttttaatgcc ttgcatacgt agtcctttat taatgtgttg
    50521 t
//
LOCUS       DAZKHK010000015        48678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKHK010000015 DAZKHK010000000
VERSION     DAZKHK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.015"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(98..802)
                     /gene="ybhL"
                     /locus_tag="ACHOS5_000944"
     CDS             complement(98..802)
                     /gene="ybhL"
                     /locus_tag="ACHOS5_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9775808.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(939..1391)
                     /gene="moaE"
                     /locus_tag="ACHOS5_000945"
     CDS             complement(939..1391)
                     /gene="moaE"
                     /locus_tag="ACHOS5_000945"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP9775809.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1393..1638)
                     /gene="moaD"
                     /locus_tag="ACHOS5_000946"
     CDS             complement(1393..1638)
                     /gene="moaD"
                     /locus_tag="ACHOS5_000946"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP9775810.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1631..2116)
                     /gene="moaC"
                     /locus_tag="ACHOS5_000947"
     CDS             complement(1631..2116)
                     /gene="moaC"
                     /locus_tag="ACHOS5_000947"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP9775811.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2119..2631)
                     /gene="moaB"
                     /locus_tag="ACHOS5_000948"
     CDS             complement(2119..2631)
                     /gene="moaB"
                     /locus_tag="ACHOS5_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP9775812.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTKGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2653..3642)
                     /gene="moaA"
                     /locus_tag="ACHOS5_000949"
     CDS             complement(2653..3642)
                     /gene="moaA"
                     /locus_tag="ACHOS5_000949"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP9775813.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3630..3774)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4039..4947
                     /gene="yvcK"
                     /locus_tag="ACHOS5_000950"
     CDS             4039..4947
                     /gene="yvcK"
                     /locus_tag="ACHOS5_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9775814.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(4985..7006)
                     /gene="uvrB"
                     /locus_tag="ACHOS5_000951"
     CDS             complement(4985..7006)
                     /gene="uvrB"
                     /locus_tag="ACHOS5_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9775815.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7585..8262)
                     /gene="bioD"
                     /locus_tag="ACHOS5_000952"
     CDS             complement(7585..8262)
                     /gene="bioD"
                     /locus_tag="ACHOS5_000952"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9775816.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8255..9010)
                     /gene="bioC"
                     /locus_tag="ACHOS5_000953"
     CDS             complement(8255..9010)
                     /gene="bioC"
                     /locus_tag="ACHOS5_000953"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP9775817.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHTQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSMALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDKIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(8997..10151)
                     /gene="bioF"
                     /locus_tag="ACHOS5_000954"
     CDS             complement(8997..10151)
                     /gene="bioF"
                     /locus_tag="ACHOS5_000954"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP9775818.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10148..11188)
                     /gene="bioB"
                     /locus_tag="ACHOS5_000955"
     CDS             complement(10148..11188)
                     /gene="bioB"
                     /locus_tag="ACHOS5_000955"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP9775819.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11275..12564
                     /gene="bioA"
                     /locus_tag="ACHOS5_000956"
     CDS             11275..12564
                     /gene="bioA"
                     /locus_tag="ACHOS5_000956"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP9775820.1"
                     /translation="MTTDDLTFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVTETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADMRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12623..13099
                     /gene="ybhB"
                     /locus_tag="ACHOS5_000957"
     CDS             12623..13099
                     /gene="ybhB"
                     /locus_tag="ACHOS5_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP9775821.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDNVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13251..14534
                     /gene="ybhC"
                     /locus_tag="ACHOS5_000958"
     CDS             13251..14534
                     /gene="ybhC"
                     /locus_tag="ACHOS5_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP9775822.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14675..16936)
                     /gene="ybhJ"
                     /locus_tag="ACHOS5_000959"
     CDS             complement(14675..16936)
                     /gene="ybhJ"
                     /locus_tag="ACHOS5_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP9775823.1"
                     /translation="MIKLSEKGVFLASNKEIIAEEHFIGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGSIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEAPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17119..18552)
                     /gene="ybhI"
                     /locus_tag="ACHOS5_000960"
     CDS             complement(17119..18552)
                     /gene="ybhI"
                     /locus_tag="ACHOS5_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP9775824.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18628..19680)
                     /gene="ybhH"
                     /locus_tag="ACHOS5_000961"
     CDS             complement(18628..19680)
                     /gene="ybhH"
                     /locus_tag="ACHOS5_000961"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP9775825.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPHADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPIVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            19864..20817
                     /gene="ybhD"
                     /locus_tag="ACHOS5_000962"
     CDS             19864..20817
                     /gene="ybhD"
                     /locus_tag="ACHOS5_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9775826.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(20858..21853)
                     /gene="pgl"
                     /locus_tag="ACHOS5_000963"
     CDS             complement(20858..21853)
                     /gene="pgl"
                     /locus_tag="ACHOS5_000963"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP9775827.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22008..22826
                     /gene="ybhA"
                     /locus_tag="ACHOS5_000964"
     CDS             22008..22826
                     /gene="ybhA"
                     /locus_tag="ACHOS5_000964"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP9775828.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(22827..23885)
                     /gene="modC"
                     /locus_tag="ACHOS5_000965"
     CDS             complement(22827..23885)
                     /gene="modC"
                     /locus_tag="ACHOS5_000965"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP9775829.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(23888..24577)
                     /gene="modB"
                     /locus_tag="ACHOS5_000966"
     CDS             complement(23888..24577)
                     /gene="modB"
                     /locus_tag="ACHOS5_000966"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP9775830.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(24577..25350)
                     /gene="modA"
                     /locus_tag="ACHOS5_000967"
     CDS             complement(24577..25350)
                     /gene="modA"
                     /locus_tag="ACHOS5_000967"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9775831.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(25513..25662)
                     /gene="acrZ"
                     /locus_tag="ACHOS5_000968"
     CDS             complement(25513..25662)
                     /gene="acrZ"
                     /locus_tag="ACHOS5_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP9775832.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            25791..26579
                     /gene="modE"
                     /locus_tag="ACHOS5_000969"
     CDS             25791..26579
                     /gene="modE"
                     /locus_tag="ACHOS5_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP9775833.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDDAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            26647..28119
                     /gene="modF"
                     /locus_tag="ACHOS5_000970"
     CDS             26647..28119
                     /gene="modF"
                     /locus_tag="ACHOS5_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP9775834.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLVGVQMPEPDEPSVRHALPAHEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GKTQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            28381..29397
                     /gene="galE"
                     /locus_tag="ACHOS5_000971"
     CDS             28381..29397
                     /gene="galE"
                     /locus_tag="ACHOS5_000971"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9775835.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            29407..30453
                     /gene="galT"
                     /locus_tag="ACHOS5_000972"
     CDS             29407..30453
                     /gene="galT"
                     /locus_tag="ACHOS5_000972"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP9775836.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGETYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAAQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            30457..31605
                     /gene="galK"
                     /locus_tag="ACHOS5_000973"
     CDS             30457..31605
                     /gene="galK"
                     /locus_tag="ACHOS5_000973"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9775837.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVNMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            31599..32639
                     /gene="galM"
                     /locus_tag="ACHOS5_000974"
     CDS             31599..32639
                     /gene="galM"
                     /locus_tag="ACHOS5_000974"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP9775838.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TDPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            32842..33594
                     /gene="gpmA"
                     /locus_tag="ACHOS5_000975"
     CDS             32842..33594
                     /gene="gpmA"
                     /locus_tag="ACHOS5_000975"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9775839.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(33760..34812)
                     /gene="aroG"
                     /locus_tag="ACHOS5_000976"
     CDS             complement(33760..34812)
                     /gene="aroG"
                     /locus_tag="ACHOS5_000976"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP9775840.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCTDVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLASAVKARRG"
     gene            35128..35508
                     /gene="ybgS"
                     /locus_tag="ACHOS5_000977"
     CDS             35128..35508
                     /gene="ybgS"
                     /locus_tag="ACHOS5_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP9775841.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSDGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            35622..36563
                     /gene="zitB"
                     /locus_tag="ACHOS5_000978"
     CDS             35622..36563
                     /gene="zitB"
                     /locus_tag="ACHOS5_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP9775842.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(36560..37279)
                     /gene="pnuC"
                     /locus_tag="ACHOS5_000979"
     CDS             complement(36560..37279)
                     /gene="pnuC"
                     /locus_tag="ACHOS5_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9775843.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFVLQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(37317..38360)
                     /gene="nadA"
                     /locus_tag="ACHOS5_000980"
     CDS             complement(37317..38360)
                     /gene="nadA"
                     /locus_tag="ACHOS5_000980"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP9775844.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLQHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(38638..38713)
                     /locus_tag="ACHOS5_000981"
     tRNA            complement(38638..38713)
                     /locus_tag="ACHOS5_000981"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38678..38680),aa:Lys,seq:ttt)
     gene            complement(38747..38822)
                     /locus_tag="ACHOS5_000982"
     tRNA            complement(38747..38822)
                     /locus_tag="ACHOS5_000982"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38787..38789),aa:Lys,seq:ttt)
     gene            complement(38871..38946)
                     /locus_tag="ACHOS5_000983"
     tRNA            complement(38871..38946)
                     /locus_tag="ACHOS5_000983"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38911..38913),aa:Lys,seq:ttt)
     gene            complement(38950..39025)
                     /locus_tag="ACHOS5_000984"
     tRNA            complement(38950..39025)
                     /locus_tag="ACHOS5_000984"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38990..38992),aa:Val,seq:tac)
     gene            complement(39077..39152)
                     /locus_tag="ACHOS5_000985"
     tRNA            complement(39077..39152)
                     /locus_tag="ACHOS5_000985"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39117..39119),aa:Lys,seq:ttt)
     gene            complement(39155..39230)
                     /locus_tag="ACHOS5_000986"
     tRNA            complement(39155..39230)
                     /locus_tag="ACHOS5_000986"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39195..39197),aa:Val,seq:tac)
     gene            complement(39266..39341)
                     /locus_tag="ACHOS5_000987"
     tRNA            complement(39266..39341)
                     /locus_tag="ACHOS5_000987"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39306..39308),aa:Lys,seq:ttt)
     gene            complement(39506..40297)
                     /gene="cpoB"
                     /locus_tag="ACHOS5_000988"
     CDS             complement(39506..40297)
                     /gene="cpoB"
                     /locus_tag="ACHOS5_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP9775845.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(40307..40828)
                     /gene="pal"
                     /locus_tag="ACHOS5_000989"
     CDS             complement(40307..40828)
                     /gene="pal"
                     /locus_tag="ACHOS5_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP9775846.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(40863..42155)
                     /gene="tolB"
                     /locus_tag="ACHOS5_000990"
     CDS             complement(40863..42155)
                     /gene="tolB"
                     /locus_tag="ACHOS5_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP9775847.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(42288..43553)
                     /gene="tolA"
                     /locus_tag="ACHOS5_000991"
     CDS             complement(42288..43553)
                     /gene="tolA"
                     /locus_tag="ACHOS5_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP9775848.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEATAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(43618..44046)
                     /gene="tolR"
                     /locus_tag="ACHOS5_000992"
     CDS             complement(43618..44046)
                     /gene="tolR"
                     /locus_tag="ACHOS5_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP9775849.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(44050..44742)
                     /gene="tolQ"
                     /locus_tag="ACHOS5_000993"
     CDS             complement(44050..44742)
                     /gene="tolQ"
                     /locus_tag="ACHOS5_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP9775850.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(44739..45143)
                     /gene="ybgC"
                     /locus_tag="ACHOS5_000994"
     CDS             complement(44739..45143)
                     /gene="ybgC"
                     /locus_tag="ACHOS5_000994"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9775851.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(45293..45586)
                     /gene="ybgE"
                     /locus_tag="ACHOS5_000995"
     CDS             complement(45293..45586)
                     /gene="ybgE"
                     /locus_tag="ACHOS5_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP9775852.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(45586..45699)
                     /gene="cydX"
                     /locus_tag="ACHOS5_000996"
     CDS             complement(45586..45699)
                     /gene="cydX"
                     /locus_tag="ACHOS5_000996"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP9775853.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(45714..46853)
                     /gene="cydB"
                     /locus_tag="ACHOS5_000997"
     CDS             complement(45714..46853)
                     /gene="cydB"
                     /locus_tag="ACHOS5_000997"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9775854.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(46869..48437)
                     /gene="cydA"
                     /locus_tag="ACHOS5_000998"
     CDS             complement(46869..48437)
                     /gene="cydA"
                     /locus_tag="ACHOS5_000998"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP9775855.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        11975 a        12992 c        12062 g        11649 t
ORIGIN      
        1 tccataaggc atattttata cctggtactt gttaagtgtc gtttttaata agaaaaaaat
       61 cccgacacat ggccgggata ttgatgatga aaaagaatta acggcggttg ccgaagatcc
      121 gcagcaacat caggaacagg ttgatgaagt ccagatacaa ggttaacgcg ccaagaatgg
      181 aatatttgcg caggttcgac gtgtcgcgag tatcaatctg ctcacccata tttttcagtt
      241 tctgggtgtc atacgccgtc aatccgacaa agacaatcac gccgatgtag gtgactgccc
      301 acatcaatgc ttcgcttttc agccagaagt taaccagcga tgccagcaca atgccgatta
      361 acgccataaa cagcatattg ccaaagccgc ttagatcgcg cttcgtggtg taaccgtaca
      421 ggctcattgc gccaaacatc ccggcagtaa cgacgaaagt actggcgata gaagcagcgg
      481 tatagacaat gaaaatactg gaaagcgtaa gacccgtcag cgccgaataa agcatgaaga
      541 gcatggttgt tacgcccgcg ctaagctttt gaatcatcgc tgataacaca ataaccaatg
      601 ctaattgcgc gatgatcaga ccgattaaaa agacacggtt agtgaacaac agctccatca
      661 cggccgcgga gttagccgca taccaggcaa caaatgcggt cagcaacaag ccaacggtca
      721 tccagccata gacttgcgcc atataagttt gcaagccagc ccggggttgt acaattgaat
      781 cagaacgtgg gaatctgtcc atgactttct cctgaagata taaggaatat cttaaggata
      841 ctgcaaaatg atgaggctgt gcatcgacgc agcgtaaacg catgtactga gcggtgaaat
      901 tgccggacgc agcggtgcct tatccggcta acaataaact accagcgttt tgccgcctgc
      961 tgatcgctct cccgagcttc gacccagcgg tcgccttccg gcgtggcttc gcgcttccag
     1021 aacggtgcgc gggttttgag ataatccata ataaactgcc cggcttcaaa cgcactgctg
     1081 cgatgcgcac tggtaacacc gacaaaaaca atttcatcgc ccggccataa ttcaccaatg
     1141 cggtgaatta ccgtgacgcg ccccagcggc cagcggttac gcgcttcatc aacgatttct
     1201 gccagtgctt tttctgtcat tcccggatag tgttcgaggg ttaatgcttt gacgctgtcg
     1261 cccaggttat ggttgcgcac cttaccggta aaggtgacta ccgcaccgtc ttcgtcacgc
     1321 tcagccagcc acgggtactc ttctcctacg ctgaacggct gcgggccgac aacaattttg
     1381 gtttctgcca tcttaacctc cggttaccgg cgggaagaaa gccacttcgt cgccgtcagt
     1441 cagcgaatgg tcaaaactca ccagcgtctg gttgacagct gccagcaatt tgccatcttc
     1501 cagcgccagc gcccagcgat cgctctgcgc agccagatgc tggcgtaacg cttcaacggt
     1561 tgggaaatcc gcagccactt cggttgcatc tgttcccacc aactcgcgca cctgggcgaa
     1621 aaaaagaacg ttaatcatcc gcttccacct taaagtcacc cgacttgccg ccgctcttcg
     1681 ccagcaaacg taccggacca atcaccatat ctttttgcac cgctttgcac atgtcataaa
     1741 tggtcagcgc cgccacggag gccgcggtta acgcttccat ttcgacaccg gttttcccgg
     1801 tcaggcggca taaggtttct atacgcaccc gattgtgctc cggctcggcc tgtaaattga
     1861 cttcaacttt gctgagcatc agcgggtgac acagcgggat cagatcccag gtgcgttttg
     1921 ccgcctgaat accggcgata cgcgcagtgg caaatacgtc gcctttgtgg tggcgaccat
     1981 caataatcat cgccagcgtc tcgctgcgca tggtgacaaa ggcttcggcg cgcgcttcac
     2041 gcacggtttc cgctttggcg gagacatcca ccatgtgcgc ttcgccagcg gcgttgatat
     2101 gggtcagttg cgacatactt atttcttcaa atgtggatgg aaattacacg gacgcgtacg
     2161 ggcatccagc tgcggcgcaa tgatattttc ccatgcggta cggcacgctt tggtcgaacc
     2221 cggcatggcg aaaatcagcg ttttgttagc gacgcccgct accgcacgag attgcaacgt
     2281 ggaagtgcca atctcttcga acgacaacat ccggaacact tcaccaaaac cttcaacttc
     2341 acggtcgaac aacggcagca gcgcttcggg agcctgatct cctttcgtca ggccagtacc
     2401 acccgtaatc aacaccactt gtacatcgtc gctggcgatc cacgcagata cctgagcgcg
     2461 aatagcgtag cggttttctt tcacaatggc tttatcgaca acgtgatggc ccgcttcttg
     2521 cgccgagtcg cgcagatagt gaccggaggt atcgtcttct tcaccgcgac gattagaaac
     2581 cgtaagaata gcaatacggg tcgggataaa ttcagtgctt acctgactca tctgatctct
     2641 ccttttgacg ttttagccac caatgtacga taagttttgc gtaataccgg tgttattttg
     2701 atgcaggaaa tgggtctgtt ttttctcccg cagcgccgct gaaatacgcg cttccagcgc
     2761 ctgttgctgg gtatcgtctt ccagcagatc gcgcaggtta acgccgcctt caccaaacag
     2821 gcagagatgg agtttaccaa tggaggaaac gcgcaggcgg ttgcaagtgg cgcagaagtc
     2881 tttttcatac ggcatgataa ggccaatctc tccggcgtaa tccggatgac aaaagacttg
     2941 cgcgggaccg tcgctgcgtt gacgtaattg gtggatccag ccgcgacgca gtagctcgtc
     3001 acgcagaacc tgaccagaga tgtgatgctt acggaagagc tcgctgccct cgcccgtttc
     3061 catcagttcg atgaaacgca gctgaatggg acgatgctgg atccagttca gaaaggtgtc
     3121 gagctggtga tgattaacat cacgcatcag cacggtattg actttaacct tctcaaaacc
     3181 ggcctcaaat gcagcatcga ttcctgccat gacctggttg aatttatcct gcccggtaat
     3241 ggcatgaaac tggcgagcgt ccagactgtc gacgctgacg ttgatgccag taagtccagc
     3301 atcgcgccag ttcgccacat cgcgctccag acggtaacca ttggtggtga ccgctatctg
     3361 gcgaatagcg tcgttttctc gcacagcagc gatgatatcg gtaaagtcgc ggcgtaaaga
     3421 tggctcacca cctgtcagac ggactttttc ggtgcccaga ctggcgaagg cgcgcgtaac
     3481 tcggcgaatt tcatcgacgg tcagaaagcc tttattggtg acgccgctcg gcttgtaacc
     3541 atccggcagg cagtaggtgc aacgaaagtt acacacatca gtaatcgaca ggcgcaagta
     3601 gtaaaactta cgcgcaaatg catcagtcag ttgtgaagcc atgtacacct ttccagatac
     3661 gggaggcgaa gtcatttctt ccttcgccct ggtggcaatc tttacgagca ttgtcacggc
     3721 caaagcaccg tatcagttga cccaggtgca gaggctagag tgtttagtgg ttaagccgat
     3781 actagcgtgt aaatgggatt tttaccatcc acactttcgc tatgtaatca tgtatatagc
     3841 gtcatgatcg tgctatttcg ctacagaata agggaaaatg acggttttac tttgatatac
     3901 atcatcaggg aggcatagcg tgcatcgtcc tttagaggta gaagaaaata gtttgtatcc
     3961 tcaatattgg caggttaatt gctgtttcct cgcaatttgc gctactgtag cgcggataat
     4021 ctgactccag gaaactatat gcgcaatcgt acgctggctg accttgatcg tgtcgttgct
     4081 ctcggcggag ggcatggact gggacgcgtt ctctcatcac tttcgtcttt gggttctcgt
     4141 ttaacgggta tcgtcaccac caccgataac ggtggctcga cggggcgtat tcgccgctca
     4201 gaaggcggta ttgcctgggg cgatatgcgt aactgcctca accagctgat aactgaaccc
     4261 agcgtcgctt ccgcaatgtt tgaatatcgt ttcggcggta atggcgaact ttccggacac
     4321 aaccttggaa acctgatgct gaaggcactg gatcacctta gcgtgcgacc tcttgaagcg
     4381 atcaatctga ttcgaaatct gctgaaagtg gacgcacatt tgattccgat gtcggagcat
     4441 cctgttgatc tgatggcgat tgacgatcag gggcatgaag tttacggcga ggtcaatatc
     4501 gaccagttaa ctacgccgat tcaagagtta ttgttaacgc cgaatgtacc cgcaacgcgt
     4561 gaggcggttc acgctatcag tgaagcggat ctcatcatta ttgggcctgg cagtttttac
     4621 accagcctga tgccaattct gctgctgaag gaaatcgcac aggcattacg ccgcacgcca
     4681 gcgccgatgg tctatatcgg caatctgggg cgtgaattga gtttacctgc ggctaatctg
     4741 aagctggaaa gcaagctggc aattatggag caatatgttg gtaaaaaggt aattgatgcg
     4801 gtcatcgtcg ggcccaaagt ggatgtctcg gcggtgaaag agcggattgt gattcaggaa
     4861 gtgctggagg ccagcgatat tccgtatcgt catgaccgcc agttgttgca tagcgcgctg
     4921 gaaaaggcat tacaggcttt aggttaacac gaagccggaa gaatattccg gcttcgtcat
     4981 cttgtcacga cgcggcaata aacagctcac gcaactgatg caactggtca cgaatttgcg
     5041 ccgcttcttc gaactccaga ttctgcgcgt gttgcatcat caacccttcc agttcatgga
     5101 tcttctgctg taacgcttta ggcgacatat ccatcggcac attatccggt tcaacaatcg
     5161 ggcgcgattt tcctctgccc ttcgctttgg ttttggcaat gttctgcccc agcgccagga
     5221 tatcgaccac tttcttgttc aagccttgcg gcgtaatgcc gtgttcttcg ttgtacttct
     5281 gctgtttctc gcggcgacgt tcggtttcgc caatcgcttt cgccattgat ggggtgatct
     5341 tatcgccgta gagaatcgct ttaccgttaa cgttacgtgc cgcacgacca atggtctgga
     5401 tcaacgaacg ttcggaacgc aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg
     5461 aaacttccgg catatccaga ccttcgcgca gtaagttgat ccctaccaat acgtcgaact
     5521 cacccagacg caagtcgcgg ataatctcca tacgttcgac ggtgtcgata tctgagtgaa
     5581 gataacgcac gcgctcaccg tgttcttcga gatattcagt gagatcttcc gccatccgct
     5641 tggtcagagt tgtaaccagt acgcgttcgt taattgccgc tcgctgacga atctccgata
     5701 gaagatcatc cacctgtgtc gctaccggac gcacttcaat aatcgggtcg agtaagcctg
     5761 ttggacgcac aacctgatcc accacatcgc caccggattt ttccagctcg taattacctg
     5821 gcgtcgccga aacatagatg gtttgcggcg ctaatgcttc gaactcttca aatttcagcg
     5881 ggcggttatc cagcgctgat ggcaagcgga agccgtactc caccagtgtc tctttacgcg
     5941 cccggtcacc gcgatacatg ccgccaattt gtggaatggt gacgtgagat tcatcgacca
     6001 ccagcagccc atcggcaggt aagtaatcaa acagcgtcgg cggtggctca cccggtccac
     6061 gaccggagag gaagcgcgag tagttctcaa tccccgaaca gtagcctagc tcgttcatca
     6121 tctccagatc aaactgggta cgctgggtca gccgctgctc ttccagcagt ttgttgtttt
     6181 ccaatagcac tttacgtctg gcagccagct cttctttgat ctcctccatc gcctggacga
     6241 tgcgctcgcg tggcgtgacg tagtgcgttt tcgggtagat ggtaaaacgt ggaatagtgg
     6301 aaacaatctg cccggtcagc gggtcaaata acgacaatcg ttccacctcc tcgtcaaaca
     6361 gctccacgcg aagtgcaatg tcatccgatt ctgccgggaa gatatcaatc acctcgccac
     6421 gaacacggaa agtaccacgc tggaatgctt gatcattacg agcatattgc agctccgcca
     6481 gtcggcgcag gatcgcgcgc tgatcgataa tcataccgac cgtgagatgg agcatcatct
     6541 tgagatataa atcaggatcg cccagaccat aaatcgcgga aacagacgcc accacaacca
     6601 catcacgccg ctccagcatc gctttggtgg cggacaaacg catctgttcg atatgttcgt
     6661 ttaccgaggc atctttctca atgaaggtgt cggaactcgg tacataggct tccggctgat
     6721 agtagtcgta gtaggagacg aaatattcca ccgcgttttc cgggaagaac tctttcattt
     6781 cgccgtacag ttgggccgcc agcgttttgt tgggcgccag taccatggtt gggcgctgaa
     6841 ggtcagcaat gacattggcg atggtgaagg ttttcccgga gccagttaca ccgagtaacg
     6901 tctggtgcgc caggccatct tccagcccct cttcgagacg tcgaatcgcc tctggctgat
     6961 cgccagaagg tttaaaagcg gaattcagtt tgaacggttt actcatgagt cgctacctga
     7021 aggagttggg cgggcaggta tgtaatttta ctcgtcgtac ttaattatgc caacaaatta
     7081 tactggataa aaaaacagtt tctcaccata atatttctgt tacagcgtaa actccgctgt
     7141 caatcatgag caaaatttac tctgtggcga gataaaactc cggccttacc gggttatccc
     7201 caaagcaacg gcttttttaa catttgtcaa gatgagtgat gacagtttta tggcaagaga
     7261 tgcctcttca gtgactgcca ttgcaattat ctaaccagtt aaaaaataaa agatatttct
     7321 ttgagccgtc tttaacgcca attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt
     7381 tttctaactc taatacacat ggttatccac aggaatagtg gataactgtc tgcagcccct
     7441 atcccccgcc gcctgggcaa ttcccacatc caccgcatga tgcggaagta aaattttttt
     7501 cgtgattttt ttagaattta attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc
     7561 gcaatttaac caagtacagg atgactacat taaggcaaga ttgatgtact ttccggttgc
     7621 cgcattttct ggattttctg caagccaggg aatttcgccc agcagcggag cgggaatcat
     7681 gcgggtgagc gtggtcatat attcagcgtg acgttttccc ggaggcgtga catcgttcgc
     7741 cacccacccc gccagagtca gtccggcgtg tagtattgcc tgcgcagtca acatcgcgtg
     7801 attaatacag ccgagtttca caccaactac cagtatcacc ggcagttgtt cctgtgttac
     7861 ccaatccgca aaagtgaaag tgtcagaaag cggcgtaaac cagccgccag caccttccac
     7921 taacacccag tcagcctgtt gttcaagcgc gcgtaatccg gcgctcatta ccaatgattc
     7981 tatcggcctg ccctcttgcg cgctgatgat gtgcggcgag gtgggttctg cgaaggtgta
     8041 aggatttacc gttgcgtaat ccagctgcag gctactgttg cgctgtaacg ccagcgcgtc
     8101 actattacgt aagcctttcg gggtcttttc gctgccagag gccaccggtt tataacctgc
     8161 cgtccggtag cctgctgcct ttgcggcttg taaaagcgcg cagctggcga cagttttccc
     8221 cacttcggta tccgttccgg tgacaaaata acatttactc acgagcaatc actcccaaaa
     8281 aaagatgata cgtcagagga tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct
     8341 gcaactgcga acgcgttaat attcgcgggt cacgcccttc atgaagatgc gtggcaccga
     8401 tgcctttcag cgaacgcatg gcactgagcg catcatcaaa ccacagcgtg atgggctgaa
     8461 tataatgttg ataatgaacg gcgtttagcg actgttcgat tttatctggc ggtaaaaagc
     8521 gattagcatg cgggcgctcg tccaccgcct gccacgcctg atgcagttcg ggtaacgatc
     8581 cctgcaccag cgtggtaaac gcgaccacgc ctccggggcg caccacccga tacagctcgc
     8641 ggagtgccat ggataaatta ccgcaccact gcaccgcgag attgctccat gcaagatcga
     8701 acgtcgcagt cgctaacggc agggattcga tatctcccgc cagataatgg tctgcggcat
     8761 ccttctggcg tgcctgaaca agcattggcg gagagagatc taacgcagtc acctgcgtgt
     8821 gacgctctcg ccagcggcgg ctcatccagc ctggtccaca acccgcatcc agtacgtggg
     8881 tgtatttacg ctgtgggagc attgccagta aggcgtcagc actctggcgc tgtagatcgg
     8941 catgttgctc atagtgtgcg gctgcccgtc caaatgccgc agcaatggct tgtttattaa
     9001 ccgttgccat gcagcacctc cagcagacgg tcgatatcct gcatttcatg cgctgcggtt
     9061 agcgttaagc gcagtcgcgc agtaccagcg ggtacggttg gtgggcgaat tgccgtgacc
     9121 caacagcctt gctggcgcag tttttctgcc agttgtaacg cacggctgtt atcaccgacg
     9181 atcaatggct ggatggcgct gcatgagtca gcaagcgtaa acggcaaatc ctgcactccg
     9241 gcacgaaaac gagtaatgag tgccgccagt ttttcgcgcc gtgcatcacc ctcatcactg
     9301 cgaatgaccg ccagcgacgc acgtaatgcc tgcgcctgag cgggcggcat actggtgctg
     9361 tagataagat ggcgggcgaa ttgcagcaga taatccgcca ccgtattgga gcaaagcacc
     9421 gctgccccgc tgacgccaaa tcctttgcca aacgtcacca ccagcaattc tggttttacc
     9481 ttttgcagcc agcagctgcc acgcccctgc tccccgataa cgcccgtgcc gtgggcgtca
     9541 tcgaccatca accagccatc gtgctgttgc gttacctgct ggatttcctc cagtggcgca
     9601 ctatcgccgt ccatgctgaa cacaccttct gtcactacca gttgctgccc cggacagggg
     9661 gaagcaagca gtcgcgccag atgagtgaca tcgttatgag caaaacggcg aagcggcgac
     9721 gggcttaaac ttgcagcctc cagcaatgag gcatggctaa gccggtcggc aacaatacgg
     9781 tcctctttcg ccatcatcgc ggcaatgaca gcctgattag cggcaaaacc agaaataaac
     9841 agcagtgccc gcgaatagcc cagccattcg gccagttctt cttccagcac ctggtgcgcc
     9901 acgctataac cgctgacgtg accggaaccg ccgctaccga cgccaaattg ttccgccccc
     9961 tgctgccagg cgcggataat ttgcggatga tggcttaaac ctaaataatc gttactggaa
    10021 aagttcagat actggcaatc atctgccacc agccagcgtc cggctccttg tgccaccgga
    10081 taacggcgac gcaaggcatc agcaacacgc cgcgcatcaa gcgccgcgtc gattttctcc
    10141 tgccagatca tagtgctgcc gcgttgtaat attcgtcagt gtccggggtc atcagcgcct
    10201 gctccagacg ctgctgttgt tcgttatcgc ccgccagcac ggcagtttgc tgcggattta
    10261 gccccagttt gcggaacagt tgcaggtctt tatcttcttc cggattcggc gtggtcagca
    10321 gtttgcaacc gtagaaaatc gagtttgcgc cagccataaa gcacatcgcc tgagtctgtt
    10381 cgttcatctg ctcgcgtccg gcagaaaggc gtacgtaaga ggtcggcatc atgatccgcg
    10441 ctacggcaat ggtacggata aaatcaaagg catcgacatc atcgttatca gcaagcggtg
    10501 tgcctttcac cttcaccagc atgttgattg gcacgctttc cggcggcgtc ggcaggtttg
    10561 ccagttgcag caataatccg gcgcgatctt ttactgtttc gcctaaaccc acaatgccac
    10621 ctgagcagac tttgatcccg gcgtcgcgca ctttttccag cgtatcgagg cgttcctgat
    10681 aggtacgcgt ggtgatgata ttgccgtaaa actccggcga ggtgtcgagg ttgtggttgt
    10741 agtaatccag cccggcattc gccaggcgct gcgcctgaga ttcactcaac gtgcccagcg
    10801 tcatacacgc ctccagcccc atcgctttta ccccctgcac catttgttcc aggtacggca
    10861 tatcacgttc gtggggattc ttccacgccg cgcccataca gaaacgtgtc gatcccgccg
    10921 ctttcgcttt gcgcgccgac tccagcacct gttcaacttc catcaaccgc tcggcttcca
    10981 gcccggtttt gtagcgcgag ctttgcgggc aatatttgca atcttccgga caagctccgg
    11041 tcttaatcga cagcaaagtg ctgacctgca cctgacgagg atcgaaatgc tgacgatgca
    11101 cctgctgcgc ttcaaacagc agatccagca acggtttttc aaataattct gtgacttgcg
    11161 acaatgtcca gcgtgggcgg tgagccatgg ggcttctcca aaacgtgttt tttgttgtta
    11221 attcggtgta gactcgtaaa cctaaatctt ttcaatttgg tttacaagtc gattatgaca
    11281 acggacgatc ttacctttga ccaacgccat atctggcacc catacacatc catgacctcc
    11341 ccgctgccgg tttatccggt ggcaagcgcc gaaggttgcg agctaatttt gtctgacggc
    11401 agacgcctgg ttgacggtat gtcgtcctgg tgggcggcga tccacggtta caatcacccg
    11461 cagcttaatg cggcgatgaa gtcgcaaatt gatgctatgt cgcatgtgat gtttggcggt
    11521 atcacccatg cgcccgccat tgagctgtgc cgcaaattgg tggcaatgac gccgcaaccg
    11581 ctggagtgcg tttttctcgc ggactccggc tccgtagcgg tagaagtggc gatgaaaatg
    11641 gcgttgcagt actggcaggc caaaggcgaa gcgcgccagc gttttctaac cttccgcaat
    11701 ggttatcatg gcgatacctt tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac
    11761 agcctgtgga aaggctatct gccagaaaac ctgtttgccc ccgccccgca aagccgcatg
    11821 gatggcgaat gggatgagcg cgatatggtg ggctttgccc gcctgatggc agcgcatcgt
    11881 catgaaatcg cggcggtgat cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg
    11941 taccatccgg aatggttaaa acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg
    12001 attgccgacg agatcgccac cggatttggt cgtaccggca aactgtttgc ctgtgaacat
    12061 gcagaaatcg cgccggatat tttgtgtctc ggtaaagcct taaccggcgg cacaatgacc
    12121 ctttccgcca cgctcaccac gcgcgaggtt acggaaacca tcagtaacgg cgaagccggc
    12181 tgctttatgc atgggccaac ttttatgggc aatccgctgg cctgcgcagc agcaaacgcc
    12241 agcctggcga ttcttgaatc tggcgactgg cagcatcagg tggcggctat tgaagcgcag
    12301 ctacgcgagc aacttgcccc cgcctgtgac gccgaaatgg ttgccgatat gcgcgtactg
    12361 ggggcaatcg gcgtggtcga aaccactcgt ccggtgaata tggcagcgct gcaaaaattc
    12421 tttgtcgaac agggtgtctg gatccggcct tttggcaaac tgatttacct gatgccgccc
    12481 tatattattc tcccgcaaca gttgcagcgt ctgaccgcag cggttaaccg tgcggtgcag
    12541 gatgaaacat ttttttgcca ataacgggaa gtcagcgtga gggtttctgg ctacactttc
    12601 tgcaaacaag aaaggaaggt tcatgaaact catcagtaac gatctgcgcg atggcgataa
    12661 gctgccgcat cgtcatgtct ttaacggcat gggttacgat ggcgataata tttcgccgca
    12721 tctggcgtgg gataatgttc ctgcgggaac gaaaagtttt gttgtcacct gctatgaccc
    12781 ggatgcgcca accggctccg gctggtggca ctgggtagtt gttaatttac ccgctgatac
    12841 ccgcgtatta ccgcaagggt ttggctctgg tctggtagca atgccagacg gcgttttgca
    12901 gacgcgtacc gactttggta aaaccgggta cgatggtgca gcgccgccga aaggcgaaac
    12961 ccatcgctac atttttaccg ttcacgcgct ggatgtagaa cgtattgatg tcgatgaagg
    13021 tgccagcggc gcgatggtcg ggtttaacgt tcatttccac tctctggcaa gcgcctcgat
    13081 tactgcgatg tttagttaat cactctgcca gatggcgcaa taccatctgg tatcacttaa
    13141 aggtattaaa aacaactttt tgtcttttta ccttcccgtt tcgctaaagt tagtataaaa
    13201 aagcaggctt caacggattc atttttctat ttcatagccc ggagcaacct gtgaacacat
    13261 tttcagtttc ccgtctggcg ctggcattgg cttttggcgt gacgctgacc gcctgtagct
    13321 caacaccgcc cgatcaacgt ccttctgatc aaaccgcgcc tggtacctct tctcgcccga
    13381 ttctgtcggc aaaagaagcg cagaatttcg atgctcaaca ctattttgca tccctgacac
    13441 caggtgcggc agcgtggaat ccttccccga ttaccctgcc tgcgcaacct gactttgttg
    13501 tcggcccggc gggtactcaa ggtgtaacgc ataccacgat tcaggcggcg gtagatgcgg
    13561 caattatcaa gcgtaccaac aagcgccagt atattgccgt gatgcctggt gagtatcagg
    13621 gaacggtgta tgtccctgcc gctccgggtg gaattactct atacggtaca ggtgaaaaac
    13681 cgattgatgt gaagattggg ctttcccttg atggtggcat gagccctgcc gactggcgtc
    13741 acgacgtcaa cccgcgcggc aaatatatgc caggtaaacc ggcgtggtat atgtacgata
    13801 gctgccagag taaacgcagc gacagtatcg gtgttctctg ctctgcggtc ttctggtcac
    13861 aaaacaatgg cctgcaactg caaaacctga ccatcgaaaa cacgctgggc gatagcgtag
    13921 atgcgggtaa ccatccggcg gtggcactgc gtactgatgg cgacaaagtg cagatcaata
    13981 acgtcaacat tctcggtcgt cagaacacct tctttgtcac caacagcggt gtgcagaacc
    14041 gtctggaaac gaatcgtcag ccgcgtacgc tggtgaccaa cagctatatt gaaggggatg
    14101 tggatatcgt ttctggtcgc ggcgcagtgg tgttcgataa caccgaattc cgcgtggtga
    14161 actcccgtac ccagcaagaa gcgtatgtgt ttgcaccggc tacgctgtcc aacatttact
    14221 acggtttcct cgccgtaaac agccgtttca atgcttccgg tgatggcgtg gcgcaactgg
    14281 gccgctcgct ggatgttgat gccaatacca acggtcaggt agtgatccgt gatagcgcca
    14341 tcaacgaagg ttttaacacg gcgaaaccgt gggccgatgc ggtgatttcc aatcgtccat
    14401 tcgcgggtaa caccggcagc gttgatgata acgacgaagt acagcgtaat ctgaatgaca
    14461 ctaactacaa ccgcatgtgg gaatacaata accgcggcgt gggtagcaaa gtggttgcag
    14521 aggcgaagaa gtaggagctg ttaactatgt gccggatgcg gcgtaaacgc cttatccggc
    14581 ctacagttcg atacgatttg tcggtcggat aagatgcgcc agcatcgcat ccgacaataa
    14641 gtgccggatg cgacctacat tcacatggcg ctttttacat ctgacggttt ttattgaagt
    14701 taatcaaact acccgccttg ataatctcgc gctcttcagc agtcagactt tccatataga
    14761 gcgtaatctc cgttaccggt gcatcttcat ggatcacata acctttaaac gtcgttccag
    14821 gattatccag cgccgcttta atgccaggga tgtaaatgta atcccccact tcaaaggttg
    14881 gcgcttccgc catctgcagc ggtaacatcc cccagttgat gacgttagaa cgatagcgtt
    14941 tagtcgcgta ctcctcggca atattcgcca gaccgccaat cacacgctga caactcgccg
    15001 cctgttcacg cgcagaacca tcgcctggtt tcaccgcata gaccatactg ccaatttcag
    15061 tttgcagcgg atcaatatgc tcctgaccag caatctgctt aatgcgcgca aacacctctg
    15121 tcagctcgct gacatttccc gccagacgct gattttccag ctcagcagtc gctttacttc
    15181 taccaacata acctggatcg cggcgtgaca gggtaaactc cgccagacca atcggatttg
    15241 aacgataaga agaggtttca ccggaaggaa tcagttcgtc ggtggtggtc acttcgtcga
    15301 ggatcttcga gcacactttg aggacgatat tgtcagtcag cgcacccaat tctggccagt
    15361 ctttaatgtt cggtccgtaa atcagcggtt gctgggtcgc ccctttcaca aagccctgat
    15421 aaacacggtt tttatacggc gttacatcga aggcgtactc cggcacgttg tcccagcaat
    15481 ccagctcgct ggcagaggtt aaatagccac cgtttgccgc agtcgccgca atagaacgag
    15541 cgtccatcaa cgccaccgct gacatctgcc cattagctgg cttagagcct tcgcggttcg
    15601 ggaagttacg cgtggtgtgg cgaatactca aaccgttgtt gattggcgta tcgcccgcgc
    15661 caaagcatgg gccgcagaac gcggttctga tgattgagcc tgcgccaatc aaatctgcga
    15721 ccacaccttt tttggcgaga tccataaaca ccggctgtga tgacgggtaa accgccagcg
    15781 agaaggtgtc attgccacag gattgaccgc gcagtgcatt cgccgccgcg atgacgtttt
    15841 cgtagttacc gccagaacag cccgcgataa tcccctgctg cactttcagg cgaccgtttt
    15901 cgactttatc cagcagcgag agtttggctt taccgtgcgc cacgcgttcg gactcaattt
    15961 caatctcacg cagaatgtcg gtcaggttct ggttcagcgt gtcgatttca tagacgttgc
    16021 tcgggtggaa cggcagcgca atcattggtt tgatggcgct taaatcaacg ctgatgcagc
    16081 catcgtagta cgccatcggt tgagggttaa gctggcagta atcctggccg cgaccgtgca
    16141 gcgccagcca gttatggact tcttcatcgg tttgccagac agaacttaaa caggtcgttt
    16201 cagtggtcat cacgtcaacg ctgttgcgga aatcggtaga gagcgctgca acaccgggac
    16261 ctacgaactc catcactttg tttttgacgt aaccgttttt gaacacggca ccgatgatag
    16321 ccagcgccac atcctgcggc cccacatacg gcgcaggttt tccggtcaga tgcaccgcaa
    16381 ccacgcccgg atagtcgata tcccaggtgt cattaagcag ctgttttacc aactcaccgc
    16441 cgccctcacc gactgccatt gtccctaatg caccgtaacg ggtgtggctg tctgacccga
    16501 ggatcatttt gccgccgcct gccatcatct cacgcatata ttgatggatg accgcaatgt
    16561 gcggaggcac aaaaataccg ccataacgct gggctgccga taaaccaaaa acatgatcat
    16621 caccgttaat agtgccgccg acggcgcaga gtgagttatg gcagttggtc agcacatacg
    16681 gcagcgggaa acgttccata ccggacgctt tagcggtctg tacaatacca acaaaggtaa
    16741 tatcgtgaga ggctaatgaa tcaaacttaa ttttaagttt atccatatta ccagacgtat
    16801 tatgagaaga gagaatagac caggcaatag tgcctttttt tgcttcttct ttttttattt
    16861 caccgatgaa atgttcttcg gcaattattt ccttattact ggcgagaaac acgccttttt
    16921 cagataactt gatcatcaaa tgctccagat atctgcgcac cggaaagtgc gcagaaagat
    16981 aataaatgga tatactctat ataattcgag ttgcaggaag gcgacaagcg agtgaatccc
    17041 caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca catgcaactt
    17101 gaagtatgac gagtatattt acagcatgtt ccagccgatc agcatattcc accaccacac
    17161 gccgagggtg atatgcacca ggaaggttaa tatcgtcagt accgcaccga ccaaccacca
    17221 ggatttaata tcgttgtaac ccacgccaaa gatgaccgga cctgccgcgc cgccatagtg
    17281 agtaaccatg ccgccatagg agttagagaa caacagtgcc agcgcggtta acattaacgg
    17341 tgcgccggag acattcgcca gcatggcaaa taccggtaac atggcaacga tataggcact
    17401 accagaagcg aagaaataac gcacgatgat gctgaggaaa ataataacga agaaagcaac
    17461 gttcccgtga ccatcaaatg ccaggttatt tttaaagact tcagctaacc attcgaagaa
    17521 tttaactttc gataataagg agcttaagcc gataataccg ccgtaccaga ttaaggtatt
    17581 ccagccgcct ttatttttaa ccacgtcttc ccaggtaacg atgcccagca gcagcatagt
    17641 tgccataaca acgattgcca cggtggattc atcaaccccc agagacttac tgaaaatcca
    17701 gcccagcagc gccagcacaa agacaccgag cagcattttt tcgcggattt tcatcggtcc
    17761 cagttcggca aggcccgctt tagcgatggt tttgttatcc accttcttaa tttctggtgg
    17821 atacatggtg taaatcacca gcggggtgac cagcagcata atgatgcccg gcaatccggc
    17881 ggccagcgcc catccacccc agctaatttg caggtgcaga atgtcgttga tcattttcag
    17941 cgccagaatg ttccccgcca ttgcggtaaa gaacatatag ctggtggttt tggtgaccat
    18001 gtaaatggac atcatcaggt aatgtccgac acgacgcgga cttttttccg gttctgaccc
    18061 caaagccacc gccacgctgt tgatgatcgg taacacaatg ccgcccgcac gcgcggtgtt
    18121 agacggtgtt gccggagcca gtaccagatc gaggaatacc gtaacgtaac ccagacccag
    18181 ggtagtgcta ccaattttac caatcagcaa ataggcaata cgtttaccta agccggtggt
    18241 cacaaatgcg gcgcttaagg taaacgccga gaacaccagc caggtggtac ctgaagagta
    18301 accgcttaat acggcggtgg tcttaaacgc accgtcggat aagttaccga ccaccaccat
    18361 ggaggcagca acggcaatta acagtacgac aggttccggg aaaggcttaa tcaccagtcc
    18421 cacaatggcc gccaggtaaa taccaaaaag cacccacgcc agttcgctta atcctgccgg
    18481 agctggcata aagccaataa tacatgggat cgctaatatc agaattagct tccataacga
    18541 ttttttattc atttaatatc ctgttctttc tgtatgactc atacagaatt cctttattga
    18601 ttgtcattat cacgacaaca aaatttttca gggaagataa acttcaccgg aaaatatttt
    18661 tctggtcgtc cgaataacag atgcgcgtaa cgtcgtggca tcctgacctt cattacttaa
    18721 atgaacgtcg agcgcaccac tggggtgttc aatattgata ttgccgtatc ctacagaagg
    18781 gacgatttgt cgggtgacgg tgccttccaa tgcacaacta ctggaaatag caatagcacc
    18841 ggttatcgcc aaagcgcgat ggcaagaatg cggcataaaa taacgcacat taattgcacc
    18901 gcctttctgc gctggagaaa taagtacagg tttagggata accatattac tgacatcgcc
    18961 taagcccatt gctttacccg cttgtagacg gatagattca atgcgggcta ataatgcttt
    19021 gtcggcatcc agctctgccg gtaattcata gcctgttttt cccagatatt cagcaggaat
    19081 aatgacaatt ggcatcgcca tatcgataca ggtcaccggg acatcgtcaa aataatcaat
    19141 ctgattatct gtcgggaaga cttttccggt tttcgttccg gcggcattca ggaaagtgag
    19201 cgcaaccggt gcggcagtac ccggtacgcc gtcaattctg gcgctaccct cgtactcgac
    19261 aacaccattt ggcgtttgca catcagcttc gatgaacgta cccgtattga cgttgcggat
    19321 acgtacgcgg gtaactggcg aagtcgctgc aatcaaacca ttttcaatgg cgaatgcccc
    19381 aacgccagac agcatattgc cgcagttagg cgtggtatcg acacgttgct catggacgat
    19441 aacttgagca aacagataat cgacatctgc atgcggatcg ctggagcggc taataatggc
    19501 gactttactg gtcagcggat taccgccgcc aataccgtca atttccagat cgttaccgga
    19561 acccataatt gccatcaata ttttatcgcg ctgcgtttga tcttcgggta gatgttccgc
    19621 taacaggaac gcgcccctcg aggttccacc tcgcatcatc acgcagggta tttttttcat
    19681 aataatgccc tcaatttaaa aacataactt attgcgatat ggttacatta agggcaaagc
    19741 atctctaatg actttagctc aacgattaat aacatttttt cattaattta agttggttaa
    19801 atgcagttaa aatttttaac ggtcagccac ccaaaatcga cgcttccatt ttccggagta
    19861 ataatgaagc atgaattatc aagtatgaag gcatttgtca tactggcaga gtccagttca
    19921 tttaacaatg ccgctaaatt actcaatatt acgcaacctg cattaacgcg aagaataaaa
    19981 aagatggagg aggatttaca tatccaacta tttgagcgta caactcgcaa ggttacgtta
    20041 acaaaagcag gaaaaagatt gctcccggag gcgcgggaat taataaagaa attcgatgaa
    20101 acgcttttta atattcgtga tatgaatgct tatcatcgtg gtatggtgac attagcgtgt
    20161 attccgaccg cagtatttta ttttttaccg ctggcaatcg gtaaatttaa tgagctgtat
    20221 cccaatatta aagtgcggat tctggaacaa ggcacgaata attgcatgga atcggtgctg
    20281 tgtaacgaat cagactttgg cataaacatg aacaacgtca caaattcatc catcgatttt
    20341 accccactgg tcaacgagcc gttcgtgctg gcctgtcggc gtgaccatcc attggccaaa
    20401 aagcagcttg tagaatggca ggagttagtg ggttacaaaa tgattggcgt acgctcctcc
    20461 agcggcaacc gactgttgat agaacaacag ctagccgaca agccctggaa gctggactgg
    20521 ttttacgaag tgcgtcatct ttcgacgtca ttagggctgg tggaggcggg actggggatt
    20581 tcggcgcttc ctggtctggc aatgccccat gcgccctatt cttcgattat tggtatccca
    20641 ctggtggaac ccgttatacg tcggacattg gggattattc gccgcaaaga tgccgtgctt
    20701 tctccggcag ctgaacgctt ttttgccttg ctgattaatt tgtggactga cgataaagac
    20761 aatttgtgga ccaatatcgt cgaacgccag agacatgcgt tacaggagat aggttaatac
    20821 accggcgcag gagagactgc gccggttaaa tcagcggtta gtgcgcgttg accactaccc
    20881 acattggccc ctgcccaacc gcatagcggc ctttttcatg cagtagcccc tgctcgccaa
    20941 caatttcgta taccgagatg tggtgagatt tttgcccggc ggcaatcaga tacttgccgc
    21001 tgtgatcaac attgaagccg cgcggctggg tttccgttgg ctggaagcct tctttactca
    21061 atacgctgcc atcttccgaa acgctgaaaa cggtaatcag actggcggta cggtcgcagg
    21121 cgtataaatg acgaccatcc ggggtgatat gaatatcagc cgcccaacgg gtgtcggaga
    21181 agttttccgg catcatatcc agcgtctgga cacattcgat attgccgtgc ggatctttca
    21241 gttcccagac atccactgag ctgtttaact cattgacgca atacgcatat tgttcgtttg
    21301 gatggaatac catatgacgc gggccggccc cttcaacggt agtcacttcc gcagggtcct
    21361 gcgccaccag atgaccatca tcgctgaccg taaacaggca aatgcgatcc tgctttaatg
    21421 ccggaaccca cagcgtacgg ttgtccggtg agatattggc ggaatggcaa ccgtccagcc
    21481 cctcgaccac atcgacgacg cccactggca ggccatcttc cagacgcgtt acgctcacgt
    21541 tacccgcatt gtaagaaccg acaaagacaa actgcccctg gtgatcggtg gaaatatgcg
    21601 tcggactacc cggcagcgca gactctgcgg caaaggtcag cgcgccatcg tccggggcga
    21661 tacgatacgc caggacgcga aactcagggc gaacaccaac atagagataa cgtttgtccg
    21721 ggctgaccac catcggctgc acctgccccg gcacatcgac aacctgtgtc agcgtcagtg
    21781 cgccttcatg attcaggttc cagacgtgaa tttgctggct ctcagggctg gcgatataaa
    21841 ctgtttgctt catgaatgct cctttgcatt tagcaactga ctgtaacagc taaaattagt
    21901 cgcttttggc ggtgaatgct gaattaaaac gcagaatttt gtccaatagt ggtatcggtg
    21961 taccattcgc gcagaagaaa attcttaacc ttaatctgga atacgccatg accacacgcg
    22021 tgattgctct cgacttagac ggcaccttat tgactccgaa aaagaccttg cttccatcat
    22081 cgatagaagc cctggcccgc gctcgcgaag caggctatca attaatcatc gtcacaggtc
    22141 gccatcacgt cgctattcat cctttttatc aggcgctggc gctggataca cctgctattt
    22201 gctgtaatgg cacctatttg tatgattatc atgcaaaaac agtgctggaa gcggacccaa
    22261 tgcccgttaa taaagccctg caactcattg agatgctgaa tgaacaccac attcacggtc
    22321 tgatgtatgt cgatgatgca atggtctatg agcacccgac cggacatgtc attcgcacgt
    22381 ctaactgggc gcaaaccctg ccgccggaac agcgtccgac tttcacacaa gtcgcttctc
    22441 tggctgaaac ggcgcaacaa gttaacgccg tatggaagtt cgccctgacg cacgatgacc
    22501 tgccgcaatt gcagcatttt ggtaagcatg tcgaacatga actgggactg gagtgtgaat
    22561 ggtcctggca cgatcaggtt gatattgcac gcggcggcaa cagcaaaggt aaacgtttga
    22621 cgaaatgggt tgaggcacaa ggttggtcga tggaaaacgt cgtggcgttc ggcgataact
    22681 tcaatgatat tagtatgctg gaagccgctg gtacaggcgt ggcgatgggc aacgccgatg
    22741 acgcagtaaa agcgcgcgcc aacattgtga ttggtgataa caccaccgac agcattgccc
    22801 agttcattta tagccacctg atttaatcag gcggttatcg acacactttt aatttgcgca
    22861 tacagccaca ggccaggttt gatcgccagt tcatccctgg cccacgggct gatacgcgcc
    22921 cacagcgttt taccgccgac ttccagctcc acttccacct ggccgttgtc gtcataacta
    22981 ttaacaactt ttgcccgcaa tacgttacga atgctggttt gctgcggcgg ttgtaaaacc
    23041 agagaaacat cggaagcctg aatgcggata cgtagcgcag cttgcagcgg ttcgtccagc
    23101 ttattgaccc acaaatgctg atcgcccagc gccagcgcgg tcatcgcgta atgcggatga
    23161 tgctcaagta ctgtcacttt cagaatgcta ctttgttgct ctttcggtag ccacggattc
    23221 atcacgctac taccccagac ttcttccagc gcgccaaagg ctttcacctg accgttttcc
    23281 agtaccatca ctctgtctgc cagatggagg atctcatcca gcgaatggct gacatacaac
    23341 atcggaatgt tgatttcccg cgtcagacgt tgcagataag gcaacagttc gcgtttacgc
    23401 ggaatatcca gtgacgccag cggttcatcc agcaacagca attccggtgc tgtcagcaaa
    23461 gcccgaccaa tcgccacgcg ctgtttttcg cctccggaca ggctgcctgg taaacgatca
    23521 agcaacggtt caatgcctaa aagcgccacc agcttatcga actgattgac catgcttttt
    23581 gccatgccgt agcgcaaatt gccacgcact ttataatgcg gaaacagccg tgcatcctga
    23641 aacacatagc caacgcgacg cttttctggc gtcaggcaga tacctttatc ggcgtcattt
    23701 agtacccgac cattgaggac aatccgccct ttttgcggac gtgtcagccc actgatggcg
    23761 ttaatcagcg aggtttttcc agcaccagag acaccaaaaa tagcggtgat gccattggcg
    23821 ggcagcgttt cattaatggt caggcaatgg ttgcccaacg tctgggaaaa attcagttcc
    23881 agcatgatta gcgccccgcc cgttcacggc tgattctggc cagccattct gaaatcaaca
    23941 gggagatcat cgccagcgca atagaaataa tacacagtct cgccgcgcca ctttccccgc
    24001 cgggagtctg gatcagggta tacatggcag aaggaatggt tcgcgtttca ccaggaatgt
    24061 tcgacacaaa ggtgatggtt gcgccaaatt caccgagcga acgagcgaat gccagtaccg
    24121 taccaacaat aattcccggc aacgtcagcg gtaaggtaat agtgaagaag acacgccagc
    24181 gcccggcacc caacgttctt gcggcctgct ccagtttgat atcaacacct tccagcgcca
    24241 gacgaatcgc ccgcaccatc agcggaaaag acatgacagc ggcagcgaga accgcaccac
    24301 gccagctaaa cgcgaaagaa agaccaaacc agtcatacag acgttcaccg ataaatccgc
    24361 gccgtcccat cgaaactaat aataggtaac cgacgaccac gggcggtaac accagcggta
    24421 gatgcagtac gctgtcgagc agagctttgc ccggaaacgt gcaacgcacc agtaaccagg
    24481 caaaaaagat cccaaacggc aggctaaaca gcacagccag ggaagaaact ttcaggctta
    24541 gtaaaaccgc ctgccattct ggatcggtca gtatcattac ttggttgtaa atccgtaacg
    24601 tttaaagatt tccgctgcct gcggtccctt cagataatca taaaatgctt tcactgtcgc
    24661 attgttatgc ccttccacaa cagcaaccgg atattccact tttttatgcg aatcttccgg
    24721 gaaggtggca accactttta cccctttgct ggcaactgca tcagaaccgt aaacaatacc
    24781 cagcggcgct tcattgcgtt cgaccaacgc cagcgcgcca cgaacatctt ccgccggagc
    24841 cagtttcggg gagagcgtat cccatgcgcc cagtttttgc agtgcttctt tcgcataaat
    24901 gccagcaggg acatgttccg gatcaccaac cgcgaggcga ccgccattca gcagcgaagt
    24961 ccagttagtt ttgctgtcga tggtgaaatc tttctgctcg ctggctttcg gtgctacaac
    25021 gaccaggcta ttgccgagca gtgtctggcg cgaagccgta tcgatcgctt ttttatcaac
    25081 cgcataatcc atccatttct gatcggcaga aataaacaga tccgcaggcg cacccgcttc
    25141 aatctgacgg gccaaagtag atgacgaagc gaaagaagaa accacatcca cgcctttctc
    25201 ttttttatac tgcgtagcaa tgtcctgcat tgcgttagtc agtgatgcgg cggcgaacac
    25261 cgtgattttc ccttcatctg ccagtgcatt gccagcaaca gcgaaagaga gtgccgcccc
    25321 ggtaaacaag ttcaaccatt tacgagccat tggtaacccc ttaatgtaac gttatgtagg
    25381 cgtcaatata acggcaagtt cggaattttc ccagcagtta ttgctaacct ttgtgaggta
    25441 gatgagaaaa aatatcggca gaaaaggaag ttgagaaaaa gaaaatgccc gactaagcgg
    25501 gcattcaggg aatcaatgat tttgtccggg ctggtctttt ttaccaacac cagaaaagat
    25561 gttgaaaact tcaccaagac cgtaaatcag acccaggatg atggccatca cgacaggtac
    25621 catgattacg gcgaatacca gactttttaa taactctaac atggtcaact ccagatatag
    25681 tcatgagact attctaaccg ctaagcacag aaaagcactc cccttttgtg cggtcagctt
    25741 tgcgtggcgt tcgttttccg tcacaataag acttttgcca ggacattgtt atgcaggccg
    25801 aaatccttct cacccttaag ctccaacaaa aattattcgc cgacccgcgc cgcatttcgc
    25861 tactaaaaca cattgcgctt tccggttcca ttagccaggg agcgaaagat gccggtatca
    25921 gctataaaag cgcctgggat gccattaacg agatgaatca gttaagtgag catattctgg
    25981 ttgagcgcgc aacaggcggt aaaggtggag gcggcgcagt actgacccgc tatggtcagc
    26041 gactgattca gctctatgac ttactggcgc aaatccagca aaaagccttt gatgtgttaa
    26101 gtgacgatga cgccctgccg ctgaacagcc tgctggccgc gatctcacgt ttttcactgc
    26161 aaaccagcgc ccgtaaccag tggttcggta ccatcaccgc ccgcgatcat gatgacgttc
    26221 aacagcatgt tgatgtctta ctggctgatg gaaaaacacg cctgaaagtc gcaattaccg
    26281 cacaaagcgg cgcgcgtctg gggctggatg aaggcaaaga agtgttgata ttgctaaaag
    26341 cgccgtgggt aggtattact caggacgatg cggtcgcgca aaacgctgac aaccaattac
    26401 caggtattat tagtcatatt gagcgcggcg cagagcagtg cgaagtatta atggcgctac
    26461 ccgacgggca aacactgtgc gccacagtgc cggtaaatga agcgacttct cttcagcaag
    26521 ggcagaatgt cacggcctac tttaatgccg acagcgtgat tatcgccacg ctgtgctaag
    26581 cgtgttgaca atttgttatg aaacacgtat ccctgtcagt aatcgctgca caaaatggga
    26641 tataaaatgt catcgttgca aattttgcaa ggcacgtttc gtcttggcga cacaaaaacg
    26701 ctgcaattgc ctcggctaac gttaaacgcg ggtgatagtt gggcgtttgt cggttcgaat
    26761 ggaagcggga aatcggcgct ggcccgcgcg ctgtcggggg aacttccgct tttgaaaggt
    26821 gaacggcaaa gccagttttc ccacatcact cgtctctcct tcgagcaatt gcaaaagctc
    26881 gtcagcgacg aatggcagcg gaataacacc gatatgctta gtcctggcga agatgacacc
    26941 gggcgtacca cggctgagat cattcaggat gaagtaaagg atactccgcg ttgcatgcaa
    27001 ctggcgcagc agttcggtat taccgccctc ctcgaccgac gctttaaata cctttccact
    27061 ggcgagacgc gaaaaaccct gctgtgtcag gcgctgatgt cggagcctga cttgttgatc
    27121 cttgatgaac cgtttgatgg cctggatgtt gcctcacgtc agcagctggc tgagctactc
    27181 gcctcgttac atcagtccgg tattactctg gtactggtgc tcaatcgctt cgatgagatc
    27241 ccggaatttg tccagtttgc tggcgtgctg gcggattgca cgttagcgga aactggcgct
    27301 aaagaagaac tgctccaaca agcactcgtt gcgcaactgg cgcatagcga actgcttgta
    27361 ggtgtgcaaa tgccggagcc ggatgaacct tcagtacgtc acgccttacc cgcccacgaa
    27421 ccgcgcattg tgctgaacaa tggcgtggtt tcttataacg atcgccccat ccttaataac
    27481 cttagctggc aggtgaatcc aggcgaacac tggcaaattg tcgggccaaa tggcgcggga
    27541 aaatcgacgt tattaagcct gattactggc gatcatccgc aaggttacag caacgatttg
    27601 acgcttttcg gacgacgtcg cggcagcggc gaaaccatct gggatatcaa aaagcatatc
    27661 ggctacgtca gcagtagttt gcatctggat taccgggtca gcactactgt gcgtaatgtg
    27721 attctttctg gctattttga ttcgattggc atttatcagg ccgtttcgga tcgccagcaa
    27781 aaactggtgc agcagtggct ggatattctc ggcattgata aacgcacggc tgacgctcct
    27841 ttccatagtc tttcctgggg acagcagcgt ctggcgctga tcgtccgtgc gctagtgaaa
    27901 catccgacgt tgcttattct cgatgaacca ctacaggggc ttgatccgct caatcgccag
    27961 cttatccgcc gttttgttga tgtgctgatt agcgaaggta aaacgcaatt gttgtttgtt
    28021 tcgcaccacg ctgaagatgc gcctgcctgt attacccatc gtcttgagtt cgtgccggac
    28081 ggtgactttt atcgctatgc gctgacaaaa ataaactgag tcggtagtgc tgaccgtgcc
    28141 ggaggcggcg taaacgtgtt atccggccaa cggtttgacg catccaggca tgaaaccgcg
    28201 tctttttttc agataaaaag cgcaatcatt cataaagccg ctgttttata atcacttaat
    28261 cgcgcataaa aaacggctaa attcttgtgt aaacgattcc actaatttat tccatgtcac
    28321 acttttcgca tctttgttat gctatggtta tttcatacca taagcctaat ggagcgaatt
    28381 atgagagttc tggttaccgg tggtagcggt tacattggaa gtcatacctg tgtgcaatta
    28441 ctgcaaaacg gtcatgatgt catcattctt gataacctct gtaacagtaa gcgcagcgta
    28501 ctgcctgtta tcgagcgttt aggcggcaaa catccaacgt ttgttgaagg cgatatccgt
    28561 aacgaagcgt tgatgaccga gatcctgcac gatcacgcta tcgacaccgt gatccacttc
    28621 gccgggctga aagccgttgg cgaatcggta caaaaaccgc tggaatatta cgacaacaat
    28681 gtcaacggca ccctgcgcct gattagcgcc atgcgcgccg ctaacgtcaa aaactttatc
    28741 tttagctcct ccgccaccgt ttatggcgat cagcccaaaa ttccatatgt tgaaagcttc
    28801 ccgaccggca caccgcaaag cccttacggc aaaagcaagc tgatggtgga acagatcctc
    28861 accgatctgc aaaaagccca gccggactgg agcattgccc tgctgcgcta cttcaaccca
    28921 gttggcgcgc atccgtcggg cgatatgggc gaagatccgc aaggcattcc gaataacctg
    28981 atgccataca tcgcccaggt tgctgtaggc cgtcgcgact cgctggcgat ttttggtaac
    29041 gattatccga ccgaagacgg tactggcgtg cgcgattaca tccacgtaat ggatctggcg
    29101 gacggtcacg tcgtggcgat ggaaaaactg gcgaacaagc caggcgtaca catctacaac
    29161 ctcggcgctg gcgtgggcag cagcgtgctg gacgtggtta atgccttcag caaagcctgc
    29221 ggcaaaccgg ttaactatca ttttgcaccg cgtcgcgagg gcgaccttcc ggcctactgg
    29281 gcggacgcca gcaaagccga ccgtgaactg aactggcgcg taacgcgcac actcgatgaa
    29341 atggcgcagg acacctggca ctggcagtca cgccatccac agggatatcc cgattaagga
    29401 acgaccatga cgcaatttaa tcccgttgat catccacatc gccgctacaa cccgctcacc
    29461 gggcaatgga ttctggtttc accgcaccgc gctaagcgcc cctggcaggg ggcgcaggaa
    29521 actccagcca aacaggtgtt acctgcgcac gatccagatt gcttcctctg cgcaggtaat
    29581 gtgcgggtga caggcgataa aaaccccgat tacaccggga cttacgtttt cactaatgac
    29641 tttgcggcct tgatgtctga cacgccagat gcgccagaaa gcaacgatcc gctaatgcgt
    29701 tgccagagcg cgcgcggtac cagccgggtg atctgctttt caccggatca cagtaaaacg
    29761 ttgccagaac tgagcgttgc ggcattgacg gaaatcgtca aaacctggca ggagcaaacc
    29821 gcagagctgg gggaaacgta cccatgggtg caggtctttg aaaacaaagg cgcggcgatg
    29881 ggctgctcta acccgcatcc gcacggacag atttgggcaa atagcttcct gcctaacgaa
    29941 gctgagcgcg aagaccgtct gcaaaaagaa tatttcgccg cacagaaatc gccaatgctg
    30001 gtggactatg ttcagcgcga gctggcagac ggtagccgta ccgttgtcga aaccgaacac
    30061 tggttagccg ttgtacctta ctgggctgcc tggccgttcg aaacgctact gctgcccaaa
    30121 gcccacgttt tgcggatcac cgatttgacc gacgcccagc gcagcgattt ggcactggcg
    30181 ttgaaaaagc taaccagtcg ttatgacaac ctcttccagt gctccttccc ctactctatg
    30241 ggctggcacg gcgcgccatt taatggcgaa gagaatcaac actggcagct gcacgcgcac
    30301 ttttatccgc ctctgttgcg ctccgccacc gtacgtaaat ttatggttgg ttatgaaatg
    30361 ctggcagaaa cccagcgaga cctgaccgca gaacaggcag cagagcgttt gcgcgcagtc
    30421 agcgatatcc attttcgcga atccggagtg taagaaatga gtctgaaaga aaaaacacaa
    30481 tctctgtttg ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc
    30541 gtgaatttga ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt
    30601 gattatcaaa ccgtgatcag ctgtgcgcca cgcgatgacc gtaaagttcg cgtaatggca
    30661 gccgattatg aaaatcagct cgacgagttt tccctcgatg cgcccattgt cgcgcatgaa
    30721 aactatcaat gggcgaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac
    30781 agcttcggcg gtgtgaacat ggtgatcagc ggcaatgtgc cgcagggtgc agggttaagt
    30841 tcttccgctt cactggaagt cgcggtcgga accgtattgc agcagcttta tcatctgccg
    30901 ctggacggcg cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgttggctgt
    30961 aactgcggga tcatggatca gctaatttcc gcactcggca agaaagatca tgccttgctg
    31021 attgactgcc gctcactggg gaccaaagca gtttccatgc ccaaaggtgt ggctgtcgtc
    31081 attatcaaca gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa
    31141 cagtgcgaaa ccggtgcgcg tttcttccag cagccagccc tgcgcgatgt caccattgaa
    31201 gagttcaacg ctgttgcgca tgaactggac ccaatcgtgg cgaaacgcgt gcgtcatatc
    31261 ctgactgaaa acgcccgcac cgttgaagct gccagcgcac tggagcaagg cgacctgaaa
    31321 cgtatgggcg agttgatggc ggagtctcat gcctctatgc gcgatgattt cgaaatcacc
    31381 gtaccgcaaa ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc
    31441 gtacgcatga ccggcggcgg atttggtggc tgtatcgtcg cgttgatccc ggaagaactg
    31501 gtgcctgccg tacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag
    31561 actttttacg tttgtaaacc atcacaagga gcaggacagt gctgaacgaa actcccgcac
    31621 tggcacccga tggtcagccg taccgactgt taactttgcg taacaacgca gggatggtag
    31681 tcacgctgat ggactggggt gcgactttac tttccgcccg tattccgctt tccgatggca
    31741 gcgtccgcga ggcgctgctt ggctgcgcca gcccggaatg ctatcaggat caggccgcat
    31801 ttctgggggc ctctattggt cgttatgcca accgcatcgc caatagccgt tatacctttg
    31861 acggtgaaac cgtgacgctt tcaccaagtc agggcgtcaa ccaattgcat ggcggcccgg
    31921 aagggttcga caaacgtcgc tggcagattg tgaaccagaa cgatcgtcag gtgctgtttg
    31981 ccctgagttc agatgatggc gatcagggtt tcccgggtaa tctcggcgcg acggtgcaat
    32041 atcgtctgac cgacgataac cgtatctcca ttacttatcg cgccacagtt gataaacctt
    32101 gcccggtgaa tatgactaat cacgtctatt tcaatcttga cggcgagcaa tctgacgtgc
    32161 gcaatcacaa gttgcagatt ctggcggaag aatatctgcc ggttgatgaa ggcggcattc
    32221 cgcacgacgg cctgaaatct gtcgccggaa cctcttttga tttccgcaac gccaaaatca
    32281 tcgccagtga gtttcttgcc gacgacgatc agcgcaaagt gaaaggttac gatcacgcgt
    32341 tcttgttaca ggccaaaggc gatggcaaga aagtggcggc gcatgtctgg tcagcagatg
    32401 aaaaattgca gctgaaggtc tacaccaccg atccggctct gcaattctac tccggcaact
    32461 tcctcggcgg cacaccgtcg cggggaaccg aaccttacgc cgactggcaa gggctggctc
    32521 tggaaagcga atttctgccg gacagcccga accaccctga atggccgcaa ccggactgct
    32581 tcctgcgtcc tggcgaagag tattccagcc tgacggaata tcagtttatt gctcagtaat
    32641 gttaagccct cgtcatgagg gctttatctt catattgttc aaatcaccag caaacaccga
    32701 catatttgca actcaatatt cacaacaacc ttacactgcg acactatttt cgctatggtt
    32761 atgcgtaagc attgctgttg cttcgtcacg gcaatataat gagaattatt atcattaaaa
    32821 gatgatttga ggagtaagta tatggctgta actaagctgg ttctggttcg tcatggcgaa
    32881 agtcagtgga acaaagaaaa ccgtttcacc ggttggtacg acgtggatct gtctgagaaa
    32941 ggcgtaagcg aagcaaaagc agcaggtaag ctgctgaaag aggaaggtta cagctttgac
    33001 tttgcttaca cctctgtgct gaaacgcgct attcataccc tgtggaatgt gttggacgaa
    33061 ctggatcagg catggctgcc cgttgagaaa tcctggaaac tgaacgaacg tcactacggt
    33121 gcgttgcagg gtctgaacaa agcggaaacc gctgaaaagt atggcgacga gcaggtgaaa
    33181 cagtggcgtc gtggttttgc ggtgactccg ccggaactga ctaaagatga tgagcgttat
    33241 ccggggcacg atccgcgtta cgcgaaactg agcgagaaag agctgccgct gacggaaagc
    33301 ctggcgctga ccattgaccg cgtgatccct tactggaatg aaaccattct gccacgtatg
    33361 aagagcggtg agcgcgtcat catcgccgca cacggtaact cgttacgtgc gctggtgaaa
    33421 tatctcgata acatgagcga agaagagatt cttgagctga atatcccgac cggcgtgccg
    33481 ctggtttatg agtttgacga gaacttcaaa ccgctgaaac gctattatct gggtaatgct
    33541 gacgagatcg cggcgaaagc agcggcggtt gctaaccagg gtaaagcgaa gtaaacgtca
    33601 ttcgtttaaa aatgagaaag ccgactgcaa gtgagtcggc ttttttgttt gtcgttgcta
    33661 acaatggagc acattgcctg atgcgacgct gcgcgtttta tcaggcctcc ggtgattaat
    33721 cggatacgcc actctgacgg cgcatccgac aattaaactt tacccgcgac gcgcttttac
    33781 tgcactcgcc agttgacgta acagagcatc ggtatcatcc cagccaatgc aggcatcggt
    33841 gatgctctta ccgtaggcca gcggttcccc gctctcgagg ctctgattgc cttccaccag
    33901 atggctttcc accatcacgc caataatggc cttttcgcca ccggcaatct gctggcaaac
    33961 gtcagtacaa acatccatct gctttttgaa ttgttttgac gagttagcat ggctgaaatc
    34021 gatcatcacc tgcgctggca ggcctgcttt gttcagccct tctttcactt cagcaacgtg
    34081 cttcgcgctg tagttaggct ctttaccgcc gcgcagaatg atatggcaat cgccgttacc
    34141 gctggtattc acaatcgccg aatgccccca tttcgttacg gacaggaagc agtgcggcgc
    34201 accggcggca ttaatggcat cgatagccac tttaatcgta ccatcagtgc catttttgaa
    34261 gcctaccggg caagaaagcc ctgatgccag ttcgcggtgc acctgcgatt ccgtagtacg
    34321 tgcgccaatt gcgccccagc tcatcaggtc agcgagatat tgtggggtga tcatatccag
    34381 gaattcaccc gccgctggca gaccgctgtc gttaatatcg agcagcaatt tgcgggcaat
    34441 acgcagaccg tcgttgatct ggaagctgtt atccatatgc ggatcgttaa tcagcccttt
    34501 ccagcccacc gtagtacgcg gcttttcaaa atagacgcgc atcacgattt ccagctcatc
    34561 ttgcagctct tcacgcagcg tcagcaagcg agcggcatac tctttagccg cgacaggatc
    34621 atgaattgag catgggccaa tcaccaccaa caggcgatca tcattacctt tcaggatttt
    34681 atggatcgct tttcgggcat gggcgaccgt attcgcggca ttttcagtag cggggaattt
    34741 ttccagcaat gcgacaggag gaagtaactc tttgatttct ttgatgcgta aatcgtcgtt
    34801 ctgataattc atgtctgttc cagtgttgcc atacttatct tagtgaatgc aatacttcca
    34861 atctatatct tccgtcagaa tgtgtaaacg gggttttaca ctatgaacgg attaatcctg
    34921 gaattagcaa gaaaaacgcc agattgtcgc gaaaaacgag atctctccta caatttctaa
    34981 ctgtaactcc tttgtttgtt aattatttca agattctctg ctgggtatct ttgcctggct
    35041 gaaatcttaa accattgcct tatattcacc tgcaaatgca ctgttggaag aggttatccg
    35101 acataacgac cataacagga gcatcctatg aaaatgacaa aactggccac actatttttg
    35161 actgccactc taagccttgc cagcggtgcc gcactggctg ccgatagcgg agcgcaaagc
    35221 aataacggcc aggcaaacgc cgcagctgat gcgggccagg tagcccctga cgcccgtgaa
    35281 aatgtcgcgc caaataacgt cgataataac ggggtaaata ccggttccga cggcacaatg
    35341 ctgcatccgg atggttcttc aatgaacaat gacggcatga ccaaagatga agagcacaag
    35401 aacaccatgt gtaaagatgg tcgctgtccg gatatcaata aaaaggtaca aactggtgat
    35461 ggcatcaaca atgatgtcga taccaaaacc gacggtacca cgcagtaacc ctgcgaaata
    35521 gccaataatg ggagagcgga gtctctccct tttaattttt cccttctaaa agcagtatga
    35581 tgtcataaag ttaagtgaga ataattaagg gatgactcgt catggcgcac tcacactcac
    35641 acacttcctc acacctgcca gaagataata atgctcgtcg cttgttgtat gctttcggcg
    35701 tgactgccgg gtttatgctc gtagaagtca ttggtggttt tctttccggt tctctggcat
    35761 tgctggccga tgcgggtcat atgttgaccg atactgccgc cctgcttttc gccctactcg
    35821 cagtgcaatt ttcccgtcgt cctcccacca ttcgccacac tttcggttgg ctaagattga
    35881 ccacgctcgc agcatttgtg aatgctatcg ccctggtggt gattaccatt ttgattgtct
    35941 gggaggcgat agaacgcttc cgcacaccgc gtccggtcga gggcggcatg atgatggcaa
    36001 ttgccgtggc tgggctgttg gcaaatatac tttctttctg gttacttcat cacggtagcg
    36061 aagagaaaaa cctcaacgtg cgagcagcgg cactgcatgt gctcggggac ctgctgggtt
    36121 cggttggggc tattattgcc gcgttgatta ttatctggac cggctggaca cctgctgacc
    36181 ccattctctc aatactggtg tcgcttctgg ttctgcgtag cgcgtggcga ctgttgaaag
    36241 atagtgtgaa tgaattactt gaaggtgcac cggtatcgct ggatatcgct gaactgaagc
    36301 gtcgcttgtg ccgggaaatc ccggaagtcc gcaatgtgca ccatgtgcat gtatggatgg
    36361 taggcgagaa gccggtgatg acgctgcatg tgcaggtgat cccgccacac gatcacgatg
    36421 ccttgttaga tcagatccaa cactacctga tggatcacta tcagattgag cacgccacta
    36481 ttcagatgga atatcaacct tgtcatgggc cagactgcca cctgaatgaa ggggtgtctg
    36541 gccattcaca tcatcaccat taatgggaca gcgcgcgtga acctctttcg cgtgcgctgt
    36601 tgatccacat ccggctgccg ttgagggcaa taaaggtcag gatgatgtac tccagagaca
    36661 tggcgtaaac gccctgaagt acaaaaataa cgacgctaat cacgttaata atcacccaca
    36721 acagccagtt ttccacatac ttacgcgtca tcagaatcat tgccacgata gataacacca
    36781 tcatgcatga atcccagaac gggaaagcgt ccggctgtag ttcaggcatc gcaacctgta
    36841 atcctaatgc ttgcatgatc atgactgcca cgcgggtcaa aaatgcaaac accggattga
    36901 taaataccgt catcagacca atcgaaacaa cgcaaaccgc caaccagctt agcgccttcg
    36961 gcaatggcaa ccagcgaatt ttcaactccg cctcgttctg actggtttgt cgcgaccacg
    37021 cataccagcc gtaaatattc gcggcaaaga aaaacacctg taatagcagg ctggcataca
    37081 gctgaatctg aaagaaaata atgccaaaca aggtgacgtt gatcaggcca aagaagtagt
    37141 tactgatctt ctccagactg gcaaggccaa tacacagcaa cccggcgatc gtgcctaccg
    37201 cttcgatcca tgagagatca taaccgcctg cccctattgg tatatgtacc aggatattct
    37261 gcacactaaa aaaatccatc gttcccccaa agcgtaatgt gacgcctcat tatcaattat
    37321 ccacgtagtg tagccgcaaa atccagcata cgattgagcg gcaccagcgc cctctctcgc
    37381 agcctttcat caacataaac ctcgtgattg cttccttcca gttctaatgc ctctgcgatg
    37441 gcctgaaggc cattcatggc catccacggg caatgcgcgc agctgcgaca ggttgcgccc
    37501 tcacccgcgg ttggtgcttc cagtaactct ttatctggca ccgcctgctg cattttgtag
    37561 aaaatgcccc gatcggttgc cacgataagc cgctgatgtt gcaatgtttt cgcagcagcg
    37621 atcagttgac tggtggaacc gaccgcatcc gccatctcta caatagcttg cggtgattct
    37681 ggatgcacca gtatggcagc atccgggtat tcttcttgca agcgggttaa cgcctgagtc
    37741 ttaaactcat catgcacaat gcaggcaccc tgccagcata aaatgtctgc gcccgtctgt
    37801 ttttgcacgt aacgccccag atgtttgtca ggtgcccaga tgattttttc acccaaacta
    37861 tccagatgat caataagttc gacggcaatg cttgaagtta ccacccaatc tgcgcgcgct
    37921 tttaccgcag cagaagtgtt ggcgtagacg acgacagtac gatcgggatg ggcatcgcaa
    37981 aatgcgttaa attcttcaac agggcagccg agatccagtg aacattcagc ctgaagtgtc
    38041 ggcatcagaa ttgttttttc cggactgaga attttggcgg tttctcccat aaatctcacc
    38101 ccagcaacta acaacgtaga agcgggatgc tttgcaccga agcgcgccat ttccagagaa
    38161 tcagaaatac agccaccggt ttcttctgcc agttgttgaa tttcgggatc ggtatagtag
    38221 tgggcaacca tcaccgcatt acgttctttt agtagacgtt ttatcttctc gcggtaatac
    38281 gctttttcat caatgcttaa cggcgtcggc ttcgggggga aaggataaat cgccgtgtct
    38341 ggatcaaaca tcacgctcat cttaccatct cgttttacag gcttaacgtt aaaaccgact
    38401 taagcgtgat attcgctatt atcggcgtaa tttgttttat atgctaaaca agatagtcga
    38461 atggtgggga aaagtcactc gaattacgta tgagatgccg aatgccacac tttgcgtctt
    38521 attcctccga cgtagagttg tttacgcagc aaacgcggag tatgtcatca gggtcaagaa
    38581 ttgaaattga gagatgcctg aatagcaaaa aagcgccttt agggcgcttt tttgcattgg
    38641 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
    38701 gagctaacga ccccttgcgg gattatgctg ctttaatcat tcaggatggt gggtcgtgca
    38761 ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac
    38821 ccatacgggt gttacctgaa agatttctac tcggaaccaa tgtttaaaat tggtgggtcg
    38881 tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa
    38941 cgacccgagt ggtgggtgat gacgggatcg aaccgccgac cccctccttg taagggaggt
    39001 gctctcccag ctgagctaat cacccgatac tgcgctggat actacaaact acttccaccg
    39061 aacccaccgt aatcagtggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    39121 gtcaactgct ctaccaactg agctaacgac ccggtggtgg gtgatgacgg gatcgaaccg
    39181 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactacgc
    39241 tggatactac aactaactat gttagtggtg ggtcgtgcag gattcgaacc tgcgaccaat
    39301 tgattaaaag tcaactgctc taccaactga gctaacgacc cactttttcg ttgctttcgg
    39361 tttgtttgat atcccgtggc aacggcggca tatattactg atttcagact tgagcgcaac
    39421 aaaaatttcg atgtagatca ctcaactgct tatgattcgc acgacacgac cagaaataat
    39481 gcgacttctg gtcgtgtgtt atgcattaca tcgcgttcag acgtttttgc gcctgtttag
    39541 cgccatcagt accagggtat ttactgataa cctgctggta tacggctttc gcttttgcgg
    39601 tgtcaccttt gtcctgcatg atgacgccga ctttaaacat cgcatctgca gccttcggtg
    39661 acttcggata gtttttcact accgaagcaa aataatacgc cgcatcatct tttttaccct
    39721 tgttgtagtt taactgaccc agccaataat tggcgtttgg caggtaagtt gaatcagggt
    39781 aatttttgat gaaattctga aatgccacca ttgcgtcatc ctggcgggat ttatcctgca
    39841 ccagcgcaat agctgcattg taatccgtgt ttgcatcacc gcttttcacc ggcgcgccag
    39901 cattcgcagt accagcatca gctgtcggcg tcgttgatgc cgccacaccg ctttgatcgc
    39961 cgctggttga ttgcgccgct gcaccaccgc tgctgaggct gtcgatctgc aacaggatct
    40021 gcttttgccg ctccacgacc tgattcagtt gatactgatt ttcctgaatc tgaccacgca
    40081 gggaatcaat atcggattga ttatcagaga gttgttgctg gagttgggtt aaaagctggc
    40141 tgtgagcatt agaaatacgc tcaagttgag tgacgcggtc ttcgaccgag cctgagccga
    40201 cactactgat tggtgcctga gcaaaagcgg cccaaggggc cgctatacca accagtaacg
    40261 acagactcaa tagttgatgt ctgaagttac tgctcatgca attctcttag taaaccagta
    40321 ccgcacgacg gtttttggca tacgccgctt cgtcatgacc cagtactgca ggtttttctt
    40381 taccgtaaga aacgatggag atctggtcag cagaaacgcc tttaccctgc agatacatct
    40441 taacagcgtt cgcacgacgt tcacccaggg agatgttgta ttccggagta ccacgttcgt
    40501 ccgcgtgacc ttctacggtg actttgtaag acgggttgct acgcaggaag tttgcgtgtg
    40561 catccagcat ttgagcgaag tcagaacgga tatcgtactt gtccagatcg aagtaaacga
    40621 tgttgttctg ctgcagctgt tgcatttgca gacgagcctg ctcttcggaa gacatgttgc
    40681 cgttgccgcc gttcgcatcc ataccagtgc cggcacccag catgccttcg ctgccgtcat
    40741 tgctggcgtt cttgttggaa gaacatgccg caattgccat aacaggcaga gcaatcatca
    40801 gccctttcag cactttgttc agttgcattt caatgattcc tttactattc aattaattat
    40861 tatcacagat acggcgacca ggcagggaat ttgacctgtc catcagttgc cggaagacgc
    40921 gctttgaaac gcccatctgt agaaaccaaa ttcagcacgg atcccatccc ctgagaagag
    40981 ctgtagatta ccatagtgcc gttaggtgcc agacttggcg tttcatccag gaacgtggac
    41041 gacagaactt gtacgcctcc cgttgccaga tcttgtttgg caatgtgctg ctgcccaccg
    41101 ttggagctga ccattaccat aaatttaccg tcgctgctga catccgcatc ctggttctgc
    41161 gaaccttccc aggtaatacg ttgtggcgca ccgccgttga tattcacttt ataaacctgc
    41221 ggacgaccgg cctggtcaga agtaaatgcc aggttctggc tgtcagggaa ccaggtcggt
    41281 tcggtattgt tactgcgacc atcagtcacc tggcggatct gaccagaagc caaatccatt
    41341 acgtacaggt tcaggctacc ggttttcgac aaggcgaatg ccagtttgct gccgtctggc
    41401 gagaatgcag gtgcaccgtt gtgacgcggg aatgaagcca cctgacgtac agcgccattt
    41461 gccagcgtct gaataaccag cgcggaacga ccgctttcga aggtcacata agccagttta
    41521 gaaccgtctg gtgaccacgc cggagacatc agcggttgcg gtgaacggtg aacgacaaac
    41581 tggttgtaac cgtcatagtc agatacgcgc agttcatacg ggaactgacc gccgttagtc
    41641 tgaacaacgt aggcaatacg ggtacggaac gcacctttaa taccggtcag tttttcaaat
    41701 acttcgtcac tggcggtatg accagcataa cgcagccact gcttgttcac tttgtacgag
    41761 ttctgagcaa gtacagtacc cggtgcgccg ccagtgtcaa caagttgata agcgacattg
    41821 taagaaccat ccggattcgg agtgacctga ccgacaacca cagcgtcaat gcccagcgcg
    41881 gaccatgcag ctggttgtac ttcctgcgca ctacccggct gctgtggcag acgagcacga
    41941 tctaacggat taaatttacc gctgttacgc aagtcagcag caacgatgcc gccaatatct
    42001 tcaggtgccg caccaggccc cgcccactgg aaaggaacaa caccaatagg acgaccggaa
    42061 tctacaccgc tgtcgatcac aatgcggact tcagcatgca gaactgatgc ccacagtatg
    42121 agaaaaccaa atgctactcg taatgcctgc ttcatcatat ctcccttatc tggaccgatg
    42181 gcccacgata atttagcaga atgttaacaa actaaaatac acaaaactac cagaaccccg
    42241 tggcaaccgg tgcctgatgt tgaccgtcgg aacagtcaac atcgcgatta cggtttgaag
    42301 tccaatggcg cgtttttgaa cacttcatat actgcctggc ttggtggttt cgggatcttc
    42361 gcaagtttag ctgctgccaa cgcagcttga caaagtgcgg gatcgccacc ttcaggtttg
    42421 atatccagta acataccatc gggtgccagt tttatgcgca gcgtacaggt tttgcctgca
    42481 taggacgatg cgtcatagaa cttactttcg atagcagatt taatctgccc ggcatagtta
    42541 ttgatatcgg cccctgatgc gccattgttt ttagtattac cactcccggc aggcgaagca
    42601 ttgttccctt tcgcccctcc ccccgttttc ggtgcattct taccagagct tagctcaccg
    42661 aaaatatcat ctgcctctgc ggcagctttt gctgcagcgg ctttttcagc tgctgctttt
    42721 gccgctgccg cttttttgtc tgcggcggct ttttctgccg ctgctttctt atcagctgct
    42781 gccttttcag cagccgcttt ttctgctgct tttttgtcgg ctgccgcttt ctctgctgcc
    42841 gctttcttat cagctgcagc cttttcagca gccgctttct tctcagcttc tgccttggct
    42901 ttttcagcag cttcggttgc cgctttcttt cttgcttcag ctgcagttgc ttctgccgct
    42961 tcagctttct tcttcagcgc cgcagcagca gcctcggctt ttttctgcgc ttcgactgcg
    43021 gctttggcgg cttctgcttc tgcttttttc ttcgcgtctg ccgccgcttt cttcgctgct
    43081 tcttccgcag ctttagcatc cgcttcggcc ttcgctttag catctgccgc cgctttcgct
    43141 gccgcctctt ccgcttgctt ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc
    43201 tttttctgct cctgagccgc taaccgctct ttctcaagtt gcttcaggcg ttcctgttca
    43261 gccgcttgtt tctcacgcag ttcttcagca gcctgctgct ccttcatctt gcgctgctca
    43321 tcagaacgct tcgcgcttga ttcctggctt tgcatacgtt tgtactgctc aactaccgca
    43381 cctgaatcaa ccatgacagc gtcgatggac gaaccaccgc cgcctccggc tgaagcttct
    43441 atattctcat cgaacgaact ccagatcagc gccgcaaata agatgacatg cagcactgct
    43501 gaaataatta tcgcccgctt gagcttgtcg ttttgttcgg ttgcctttga cactctcggt
    43561 ttccaaaaac tgttcgcctg ttacccgctc tctttcaagc aagggaaacg cagatgttta
    43621 gataggctgc gtcattaaac caaccgattt cacacccgca ctatgtaaca agttcagtgc
    43681 tttaattatt tcatcgtaag gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg
    43741 gttggccttg aaacggctgg acacttccgc caccacctgc tctggtggta aacgctccag
    43801 acgatctttc tcaaccacca cggtgtactg accaatacca gacacttcaa caatcactgg
    43861 cggattatcg ttactgctca ccgcctgtga ttcagtagcg tctggcagat cgacctccac
    43921 gctctgggtg atgatgggcg ctgtcgccat aaagatcagc aacagcacca gcagtacgtc
    43981 cagcaacggt acaatgttga tttcggactt gagatcgcga cgacctcgtc cacgcgctct
    44041 ggccatggct taccccttgt tgctctcgct aacggtaaac gcctggcggt gcagaatcgc
    44101 ggtaaactct tccataaagt tgtcgtaatt cagttccagt ttgtttacgc gctggttgag
    44161 gcggttataa gccattaccg ccggaattgc ggcgaacaaa ccaattgccg tcgcaatcaa
    44221 cgcttctgcg ataccgggcg caaccatttg cagtgttgct tgttttaccg ccccgagggc
    44281 gataaaggcg tgcatgatcc cccagaccgt accaaacaga ccaatatacg ggctgatgga
    44341 gccaaccgta ccgaggaacg gaatgtgcgt ttccagattt tcaagttcac gattcatgga
    44401 aatacgcatc gcacgcgacg ccccttccac tacggcttcc ggcgcatggc tattggcacg
    44461 atgcaggcgc acaaactctt tgaagccgct gtaaaagatt tgttccgaac cgctcagatt
    44521 atcccgtttc ccctggctct cttgatagag gcgagagagt tcgattccag accagaattt
    44581 atcttcaaac gcttcggctt cgcgcgccgc tgcgttaaga atacgggtcc gctggataat
    44641 aatggcccag gatgcgattg aaaaaccaat caaaatcaac atgataagtt taaccagaag
    44701 gctagccttc aggaacaaat caaggatatt catgtcagtc actgcttaaa ctccgcgaca
    44761 atagacttgg gaagcgcacg aggcttcatt ttgagtgggt caacgcaaac aaccagaacc
    44821 tctgcttcat tcaacaaagt attctcggcg ttgacaatac gttgcgtgaa aaccaaagag
    44881 gtgccacgca ttgatgttat ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt
    44941 gcgtaatatt ccaccgtcat tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc
    45001 tgttgactga aatgatgatg acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg
    45061 taactggcgt ggtacaccac accaccggca tcggtatctt catagtagac gcgaaccggc
    45121 catcgaaaca gcgttgtatt cactttacat cccggtaatg caacaaaagt tagagctttt
    45181 aaacgctgct actatacgcg cgcgattgag gtttgggaat gagggcaagt taagggagcg
    45241 taaaaattta tgggcctgac tcggcccata aatttttaaa gagaattctg atttaaaaga
    45301 agaagaaaat cagcccgaca atgagaacaa tatcggcaag caatgggcaa aaaatccctt
    45361 gccagagaac cttctgcgga cgaaagccca cgccgtgaat cacaccagca cagacggccc
    45421 acatcagcag taaaccatgc cagatttcca gttcactggt cttcgcggcg aaacgtgatg
    45481 ggtcccaaaa catacatcct gctaacagaa gtgccatcac gaaggaaagc gcccgcaggg
    45541 ggcgcttgtc cattaccgca tacaaggttg cgataatctt actcatcaga tgtcttcttg
    45601 accggctttg cctgattcga cgtgctcaag cgccagcgcg gtgattaccc caaacgaaca
    45661 ggcaagaagc gttcccagaa tccatgcgaa ataccacatt tttagctcct tacttagtac
    45721 agagagtggg tgttacgttc aatatcttct ttggtgatac gaccgaacat tttccagtaa
    45781 caccaggcgg tgtagagcag aatgatcggt accagaacca ccgcaaccca ggtcatgacg
    45841 ttaagcgtca gctggctgga agttgcatcc cacattgtca gacttgcgtt catcatggtg
    45901 ctggacggca tcacaaacgg gaacattgcg ataccggcag tcaggatgat gcaggccagc
    45961 gtcagggagg agaacacaaa cgcccacgcg gctttatcca tacgtgcagt caggatggtc
    46021 agcagcggca gaaccacacc cagtgccgga atagcccaca gaattggcgt gttgttgaag
    46081 ttaaccagcc atgcgccagc ttcacgaacc acttctttat tcagcgggtt agaggctgcg
    46141 taatggtcca ttgtcgattt cacgacataa ccatcgatac cgtacatcac ccatacgcca
    46201 gccagtgcga agcagaccag cgtcaccagt gccgccacct gagccgttgc acgggtacgc
    46261 aggtgcagtt cgcccacggt acgcatttgc agataggttg cgccctgagt aatgatcatc
    46321 cctacgctca ccacacctgc cagcaggccg aacgggttaa gcagctggaa gaagttaccg
    46381 gtgtagtaca gacgcagata ttcatcaacg ttgaatggta cgccctgcaa caggttaccg
    46441 aacgctacac caatcaccag cggcggaacg aagctaccaa tgaagatgcc ccagtcccac
    46501 atgttacgcc agcgggtttc ttcaatcttg gagcggtagt caaaaccgac cggacggaag
    46561 aacaaagacg ccagcacgag gatcatcgcc acatagaagc cggagaacgc agcggcatag
    46621 accatcggcc aggcagcaaa cagtgcgccg cctgcggtga tcagccaaac ctggttaccg
    46681 tcccagtgtg gtgcgatgga gttaatcata attcgacgct cggtgtcgtt acgaccgagg
    46741 aaacgggtga gcatgcccac ccccatgtcg aaaccgtcag tgactgcaaa accaattagc
    46801 agaacgccaa ccagcagcca ccagataaaa cgcaatactt cataatcgat catttgacga
    46861 ctcctgtctt agcgtgccgg ctgagtagtc gtggaagact gctcaaagtg atagcgaccg
    46921 gttttcaggc tgcttgggcc gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc
    46981 aggaacaggg tatacaggcc gcaaatcagc accatcgaga agatgagatc gcctgcggtc
    47041 agtgacgagt tcgccacagc tgtcggcagc acttcaccga tagcccacgg ttggcggcca
    47101 tattcagcca cgaaccagcc cgcttctaca gcaatccacg gcagcggaat accgtacagc
    47161 gcggcgcgca gcagccattt tttctcgcca atgcggtttc ggatgacact ccagaaagag
    47221 agcgcgatga ttgccagcag caggaagcca cacgccacca tgatacggaa cgcaaagtac
    47281 agcggcgcta cacgcggaat ggagtctttg gttgcctgtt gaatctgagc ttcagtcgca
    47341 tcagccacgt ttggcgtata gcgtttcagc agcagaccgt aaccgagatc tttcttcatg
    47401 ctattgaact ggtcacgaac ggcctggtca gtagaaccag aacgcagttg ttcgagcaga
    47461 gagtacgcct tcatcccgtt acgaatgcgt tcttcatgct gcaccatcag ttctttcaga
    47521 ccgataactg gagtatccac ggaacgcgtt gcaatgatgc ccagcgcgta agggatctgg
    47581 atcgcaaatt tgttcgtttc ctcttcctga tcaggaatgc cgaacagagt aaaggcagca
    47641 ggtgcaggtt gcgtttccca ctcggcttcg atagcagcca gtttggtttt ctgcacgtcg
    47701 cccatttcgt agccggattc atcacccaga acaataacag acagaacggc agccataccg
    47761 aagctggcag cgatagcaaa ggagcgttta gcgaaggcga agtcacgacc tttcagcata
    47821 taccatgcgc tgataccgag gatgaacatt gcgccagtca cataaccaga agctacggtg
    47881 tgaacgaatt tcacctgagc aaccgggtta agcaccagct cggagaagct caccatctcc
    47941 atacgcatag tttcaaagtt gaaatcggac gcgattgggt tttgcatcca gccgtttgca
    48001 accagaatcc acagtgcgga caggtttgaa ccgagcgcca ccagccaggt gacacacata
    48061 tgctgaactt tacccagacg atcccaaccg aagaagaaca gacctacaaa ggtggattcg
    48121 aggaagaagg ccatcagacc ttcgattgcc agaggcgcac cgaagatatc ccctacatag
    48181 tgggaatagt aagaccagtt agtcccgaac tggaactcca tggtcagacc ggtagccaca
    48241 cccagagcaa agttgatacc aaacaacttg ccccagaact tggtcatatc tttataaatc
    48301 tgtttgccgg aaaggacgta gaccgtttcc ataatggcca gcaggaacgc cataccgagc
    48361 gtcagtggca caaaaaggaa gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt
    48421 tcgactatat ctaacatcat gactccttgc tcatcgcatg aagactccga gagtgaaccc
    48481 cgttagaaag ggtcacacgc atgccccaat acaaaattat ttgctcgcct ctatcgttgc
    48541 catttcatca aaagaggaaa agacaatgat aaaaaagtta caaatgtgtt aatgaataaa
    48601 ccaaattgat cccgtaatta tattacgccg caaaatcctt acaataaaca ggtttttatt
    48661 gagcaaattt gcattttt
//
LOCUS       DAZKHK010000016        48224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKHK010000016 DAZKHK010000000
VERSION     DAZKHK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.016"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..385
                     /gene="yjfI"
                     /locus_tag="ACHOS5_000999"
     CDS             <1..385
                     /gene="yjfI"
                     /locus_tag="ACHOS5_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP9775856.1"
                     /translation="ALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
     gene            404..1102
                     /gene="yjfJ"
                     /locus_tag="ACHOS5_001000"
     CDS             404..1102
                     /gene="yjfJ"
                     /locus_tag="ACHOS5_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP9775857.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1152..1811
                     /gene="yjfK"
                     /locus_tag="ACHOS5_001001"
     CDS             1152..1811
                     /gene="yjfK"
                     /locus_tag="ACHOS5_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP9775858.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDAL
                     LIELPGEEYTVAAVSCIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKCWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPMTSLHII
                     G"
     gene            1829..2227
                     /gene="yjfL"
                     /locus_tag="ACHOS5_001002"
     CDS             1829..2227
                     /gene="yjfL"
                     /locus_tag="ACHOS5_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP9775859.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2237..2875
                     /gene="yjfM"
                     /locus_tag="ACHOS5_001003"
     CDS             2237..2875
                     /gene="yjfM"
                     /locus_tag="ACHOS5_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP9775860.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2878..4041
                     /gene="yjfC"
                     /locus_tag="ACHOS5_001004"
     CDS             2878..4041
                     /gene="yjfC"
                     /locus_tag="ACHOS5_001004"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9775861.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYCFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARCSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4125..5750
                     /gene="aidB"
                     /locus_tag="ACHOS5_001005"
     CDS             4125..5750
                     /gene="aidB"
                     /locus_tag="ACHOS5_001005"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP9775862.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGASWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWITPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYLLVGHKWFFS
                     VPQSDAHLVLAQAAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5867..6142)
                     /gene="yjfN"
                     /locus_tag="ACHOS5_001006"
     CDS             complement(5867..6142)
                     /gene="yjfN"
                     /locus_tag="ACHOS5_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP9775863.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6291..6620)
                     /gene="bsmA"
                     /locus_tag="ACHOS5_001007"
     CDS             complement(6291..6620)
                     /gene="bsmA"
                     /locus_tag="ACHOS5_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP9775864.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6802..7551
                     /gene="yjfP"
                     /locus_tag="ACHOS5_001008"
     CDS             6802..7551
                     /gene="yjfP"
                     /locus_tag="ACHOS5_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9775865.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7548..8303)
                     /gene="ulaR"
                     /locus_tag="ACHOS5_001009"
     CDS             complement(7548..8303)
                     /gene="ulaR"
                     /locus_tag="ACHOS5_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP9775866.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8411..9475)
                     /gene="ulaG"
                     /locus_tag="ACHOS5_001010"
     CDS             complement(8411..9475)
                     /gene="ulaG"
                     /locus_tag="ACHOS5_001010"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9775867.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPFMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9830..11227
                     /gene="ulaA"
                     /locus_tag="ACHOS5_001011"
     CDS             9830..11227
                     /gene="ulaA"
                     /locus_tag="ACHOS5_001011"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9775868.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11243..11548
                     /gene="ulaB"
                     /locus_tag="ACHOS5_001012"
     CDS             11243..11548
                     /gene="ulaB"
                     /locus_tag="ACHOS5_001012"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP9775869.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11558..12022
                     /gene="ulaC"
                     /locus_tag="ACHOS5_001013"
     CDS             11558..12022
                     /gene="ulaC"
                     /locus_tag="ACHOS5_001013"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP9775870.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12036..12686
                     /gene="ulaD"
                     /locus_tag="ACHOS5_001014"
     CDS             12036..12686
                     /gene="ulaD"
                     /locus_tag="ACHOS5_001014"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9775871.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12696..13550
                     /gene="ulaE"
                     /locus_tag="ACHOS5_001015"
     CDS             12696..13550
                     /gene="ulaE"
                     /locus_tag="ACHOS5_001015"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP9775872.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLNWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13550..14236
                     /gene="ulaF"
                     /locus_tag="ACHOS5_001016"
     CDS             13550..14236
                     /gene="ulaF"
                     /locus_tag="ACHOS5_001016"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP9775873.1"
                     /translation="MQKLKQQVFEANMDLPHYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGNVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14365..14640)
                     /gene="yjfY"
                     /locus_tag="ACHOS5_001017"
     CDS             complement(14365..14640)
                     /gene="yjfY"
                     /locus_tag="ACHOS5_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP9775874.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14968..15363
                     /gene="rpsF"
                     /locus_tag="ACHOS5_001018"
     CDS             14968..15363
                     /gene="rpsF"
                     /locus_tag="ACHOS5_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9775875.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15370..15684
                     /gene="priB"
                     /locus_tag="ACHOS5_001019"
     CDS             15370..15684
                     /gene="priB"
                     /locus_tag="ACHOS5_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP9775876.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15689..15916
                     /gene="rpsR"
                     /locus_tag="ACHOS5_001020"
     CDS             15689..15916
                     /gene="rpsR"
                     /locus_tag="ACHOS5_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9775877.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15958..16407
                     /gene="rplI"
                     /locus_tag="ACHOS5_001021"
     CDS             15958..16407
                     /gene="rplI"
                     /locus_tag="ACHOS5_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9775878.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            16809..17300
                     /locus_tag="ACHOS5_001022"
     CDS             16809..17300
                     /locus_tag="ACHOS5_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9775879.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     SSGKNEEGIQTDPFQTRYEKEGLLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            17303..18571
                     /locus_tag="ACHOS5_001023"
     CDS             17303..18571
                     /locus_tag="ACHOS5_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HFP9775880.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKTSVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMVVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGYILIPGK
                     GWRLSEAYDLNPVARSDGLKLNITENDNALDLELAREVAEYFRLGLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRGAI"
     gene            complement(18714..19991)
                     /locus_tag="ACHOS5_001024"
     CDS             complement(18714..19991)
                     /locus_tag="ACHOS5_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9775881.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATS"
     gene            complement(20084..22156)
                     /locus_tag="ACHOS5_001025"
     CDS             complement(20084..22156)
                     /locus_tag="ACHOS5_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFP9775882.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRQVFMDFNRNPEPVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQQMNPLSISLYKMHGYDLITQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRIQPCINGIYFEKNWPDF
                     LKGDIYTQ"
     gene            complement(22153..23694)
                     /locus_tag="ACHOS5_001026"
     CDS             complement(22153..23694)
                     /locus_tag="ACHOS5_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase"
                     /protein_id="HFP9775883.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SSQEVLYITERAVFQLTAEGVELIEIAPGVEIDRDILPYMAFRPIIRHPRLMESSLFT
                     PMEDA"
     gene            complement(23704..24480)
                     /locus_tag="ACHOS5_001027"
     CDS             complement(23704..24480)
                     /locus_tag="ACHOS5_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFP9775884.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQTANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILTL
                     ENKHADYSHQLLAGLMSATQDCQQATRAFAEKAGVSFHNQ"
     gene            complement(24490..25332)
                     /locus_tag="ACHOS5_001028"
     CDS             complement(24490..25332)
                     /locus_tag="ACHOS5_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9775885.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQIARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(25342..26133)
                     /locus_tag="ACHOS5_001029"
     CDS             complement(25342..26133)
                     /locus_tag="ACHOS5_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFP9775886.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            26346..26993
                     /locus_tag="ACHOS5_001030"
     CDS             26346..26993
                     /locus_tag="ACHOS5_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9775887.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(26977..27615)
                     /gene="ytfB"
                     /locus_tag="ACHOS5_001031"
     CDS             complement(26977..27615)
                     /gene="ytfB"
                     /locus_tag="ACHOS5_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP9775888.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQMFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            27833..28453
                     /gene="fklB"
                     /locus_tag="ACHOS5_001032"
     CDS             27833..28453
                     /gene="fklB"
                     /locus_tag="ACHOS5_001032"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9775889.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQTMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            28762..30165
                     /gene="cycA"
                     /locus_tag="ACHOS5_001033"
                     /gene_synonym="dagA"
     CDS             28762..30165
                     /gene="cycA"
                     /locus_tag="ACHOS5_001033"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP9775890.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            30299..30724
                     /locus_tag="ACHOS5_001034"
                     /pseudo
     CDS             30299..30724
                     /locus_tag="ACHOS5_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30829..31896
                     /locus_tag="ACHOS5_001035"
     CDS             30829..31896
                     /locus_tag="ACHOS5_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775891.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDAVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLVAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSRVYLREDDPVTLAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWENGAWEKCNWIRIRRVANRFIDVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRMAACSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(32143..32805)
                     /gene="ytfE"
                     /locus_tag="ACHOS5_001036"
     CDS             complement(32143..32805)
                     /gene="ytfE"
                     /locus_tag="ACHOS5_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP9775892.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(32913..33878)
                     /gene="ytfF"
                     /locus_tag="ACHOS5_001037"
     CDS             complement(32913..33878)
                     /gene="ytfF"
                     /locus_tag="ACHOS5_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9775893.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(33986..34846)
                     /gene="qorB"
                     /locus_tag="ACHOS5_001038"
     CDS             complement(33986..34846)
                     /gene="qorB"
                     /locus_tag="ACHOS5_001038"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9775894.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQVLTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKPIT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHFFNVNN"
     gene            34935..35315
                     /gene="ytfH"
                     /locus_tag="ACHOS5_001039"
     CDS             34935..35315
                     /gene="ytfH"
                     /locus_tag="ACHOS5_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9775895.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(35444..37387)
                     /gene="cpdB"
                     /locus_tag="ACHOS5_001040"
     CDS             complement(35444..37387)
                     /gene="cpdB"
                     /locus_tag="ACHOS5_001040"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP9775896.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSANPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            37577..38317
                     /gene="cysQ"
                     /locus_tag="ACHOS5_001041"
     CDS             37577..38317
                     /gene="cysQ"
                     /locus_tag="ACHOS5_001041"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP9775897.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(38307..38864)
                     /gene="ytfJ"
                     /locus_tag="ACHOS5_001042"
                     /pseudo
     CDS             complement(38307..38864)
                     /gene="ytfJ"
                     /locus_tag="ACHOS5_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224754.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
     gene            39189..39395
                     /gene="ytfK"
                     /locus_tag="ACHOS5_001043"
     CDS             39189..39395
                     /gene="ytfK"
                     /locus_tag="ACHOS5_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP9775898.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(39473..40816)
                     /gene="paeA"
                     /locus_tag="ACHOS5_001044"
     CDS             complement(39473..40816)
                     /gene="paeA"
                     /locus_tag="ACHOS5_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9775899.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(41139..41777)
                     /gene="msrA"
                     /locus_tag="ACHOS5_001045"
     CDS             complement(41139..41777)
                     /gene="msrA"
                     /locus_tag="ACHOS5_001045"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9775900.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            41984..43717
                     /gene="tamA"
                     /locus_tag="ACHOS5_001046"
     CDS             41984..43717
                     /gene="tamA"
                     /locus_tag="ACHOS5_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP9775901.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            43714..47493
                     /gene="tamB"
                     /locus_tag="ACHOS5_001047"
     CDS             43714..47493
                     /gene="tamB"
                     /locus_tag="ACHOS5_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP9775902.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     TVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            47496..47837
                     /gene="ytfP"
                     /locus_tag="ACHOS5_001048"
     CDS             47496..47837
                     /gene="ytfP"
                     /locus_tag="ACHOS5_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP9775903.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            48004..>48224
                     /locus_tag="ACHOS5_001049"
     CDS             48004..>48224
                     /locus_tag="ACHOS5_001049"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775904.1"
                     /translation="MFDREMINENKEAFFLYVNGRGELSAQCVIKVAEKGDYLQGFSL
                     TRRAFRTFLKDRVIQMFSSDQELNKVASET"
BASE COUNT        11837 a        11711 c        12694 g        11982 t
ORIGIN      
        1 ggcgctggcg acggcgctgc aaactgtacc tgaacaaaat attgatgtaa caaatagcga
       61 aagcgcatta attattaaaa tgaatgatta tggcgatttg caaatcaata ttctttttac
      121 ttcccgccaa atgatcatcg aaacctttat ttgtccggta agtagtatca gcaatccaga
      181 tgaatttaat accttcttat taagaaatca gaagatgatg ccgttgtcat cggtagggat
      241 ctccagcgtg caacaggaag agtattacat tgttttcggt gcgttatcgc ttaaatcttc
      301 tcttgaagat atcctgcttg agataacctc gctggtagat aacgcattgg atctggctga
      361 aattacagaa gaatattcac actaattaca aggacgggta tttatgggaa ttttaaaaag
      421 tttatttacg ctggggaaat cgtttatctc ccaggcggaa gagtccattg aagaaaccca
      481 gggcgtgcgc atgctggaac agcatattcg tgacgctaaa gctgaactcg ataaagccgg
      541 aaaatctcgc gttgatctgc tggcgcgagt gaaattaagt cacgataagc tgaaagattt
      601 acgtgagcgc aaagccagtc tggaagctcg tgcgctggaa gcgttaagca agaacgtgaa
      661 tccgtcgttg attaacgaag ttgctgaaga aatcgcgcgc cttgagaatc tcattaccgc
      721 tgaagagcaa gtgttgtcga atctggaagt ctctcgtgat ggcgtggaaa aagcagttac
      781 agcgacagcg cagcgtatcg ctcagtttga gcagcaaatg gaagtcgtta aagccactga
      841 agccatgcag cgtgcacaac aggctgtaac gacctctact gttggcgcat cttccagcgt
      901 ttcgacagcg gcagaatcct taaaacgcct gcaaacgcgt caggccgaac gtcaggctcg
      961 cctggatgct gccgcacagt tggaaaaagt cgcagacggt cgcgatctcg atgaaaagct
     1021 ggcggaagcc ggaattggcg gtagcaataa aagtagcgcc caggatgtat tagcaagact
     1081 gcaacgccaa cagggcgagt aattttttgc cagcctcact gcaaggctgg caactatttt
     1141 aaggataaaa tatgtctggt tttttccagc gtctgtttgg caaggataat aagcctgcta
     1201 tcgctcgcgg tccgttgggt ctgcatctca atagtggttt tacgctcgat acgttagcgt
     1261 ttcgtttgct ggaagatgca ttgctgatcg agctaccggg tgaagaatat accgtggccg
     1321 ccgtcagttg catcgatctg ggcgggggta gccagatttt ccgttactac acttcgggcg
     1381 atgagttcct gcaaatcaat accactggcg gcgaagatat tgatgacatt gatgatatca
     1441 agctctttgt ctatgaagag agctacggta tcagtaaaga gagtcactgg cgcgaggcga
     1501 tcaacgccaa agcgatgggg gcaatgacct taaactggca ggaaaaatgc tggcagcgat
     1561 tttttaacag cgaagaaccg ggaaatatcg aacccgtcta catgctggaa aaagtagaaa
     1621 atcaaaacca tgccaaatgg gaagtgcata attttagcat gggctaccaa cggcaggtga
     1681 ccgaagatac ttacgaatat ttgctgttaa atggtgagga atcttttaac gatctcggcg
     1741 agccggagtg gttattttcg cgcgcgctgg gtgtcgatat cccgatgaca tcacttcata
     1801 ttattggtta attacaagga cgtttacaat gcatatactg gattcacttc ttgcctttag
     1861 tgcctatttt tttattggcg tggcgatggt gattattttc ctgtttatct actccaaaat
     1921 tacaccgcac aacgaatggc agttaatcaa aaacaataac accgcggcgg cactggcgtt
     1981 cagcggaaca ttgttggggt acgtgatccc cttatccagt gcggcaatca atgcggtgag
     2041 tattccagac tatttcgcat ggggcgggat tgctctggtg attcaattac tcgtttttgc
     2101 tggcgtcagg ctttatatgc ctgcattaag cgaaaaaatt attaatcaca ataccgcagc
     2161 aggaatgttt atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac
     2221 atggtaatgg acgatcatgg ccagaaaacg caaatccaga aataacaaaa atatcggaca
     2281 tggggcaatc agtcgcattg gtagaccgca taatcctttt gagccgcgcc gcaatcgcta
     2341 cgcacaaaaa tacttaacgt tggcactaat gggcggtgcc gcttttttcg tattgaaagg
     2401 ttgtggcgat agtggcgatg tcgataacga cggcgacgga acgttttacg cgactgtgca
     2461 ggattgtatt gatgatggta ataattcaga tatctgtgct catggctgga acaacgccaa
     2521 agcggcattt tatgccgatg ttccgaagaa tatgactcag cagaattgtc agtctaagta
     2581 cgaaaattgc tactacgaca atgttgaaca gagttggatc ccggtcgttt ctggattttt
     2641 gttaagccgg gttattcgta aagatcgcga tgagccgttc gtctataaca gtggcggatc
     2701 ctcatttgct tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgctccgg
     2761 ttctggcaaa aaagagtctt actcttcggg cggctttacc accaaaaaag cgtcaaccgt
     2821 ttctcgcggc ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg
     2881 ctgagacaca acgttcctgt gcggcgggat ctggaccgga ttgccgccga taacggattt
     2941 gactttcata tcatcgacaa tgaaatctat tgggatgaga gtcgggctta ctgctttact
     3001 ttgcgccaga ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctg
     3061 gaggtggtgg atcgcgcggt taaagatgaa gagatcctga cgcaactggc gatcccgccg
     3121 ttgtactggg atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgtt gtatggccgt
     3181 atggattttg cctggtgtgg taatgcaccg gttaagttgc tggagtacaa cgccgatacg
     3241 ccaacttcat tgtacgagtc ggcttatttc cagtggttgt ggctggaaga tgcccggtgc
     3301 agcggcatta ttccgcgcga tgccgatcag tacaatgcca ttcaggagcg cctgatttca
     3361 cgctttagcg agctttacag tcgggaaccg ttttattttt gctgctgtca ggataccgac
     3421 gaagacagga gtaccgtgct gtacttgcag gactgcgccg agcaggcagg gcaggagtct
     3481 cggtttatct acattgaaga gctcggtttg ggcgtcggcg gcgtactgac cgatcttgat
     3541 gataatgtca tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat
     3601 aacggcccgc tgctgcgcaa gcgtcgtgag caatgggtgg agccgttatg gaaaagtatc
     3661 ttgagtaata aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt
     3721 cttgcatcct ggttcgatgg tgaaaaaccg cagattgccg ctggcgaaag ctatgtgcgt
     3781 aaaccgattt actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtt
     3841 gttgaccacg ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct
     3901 ctgccgcgat ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg
     3961 tgcggaatgg ggatccgtga agataacacg ctgatcacca aagacacctc acgtttcgtt
     4021 ccgcattaca ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc
     4081 agtgaccata ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc
     4141 acaccgtttt taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg
     4201 cgctttgcga agcggtaacg cgtgaaggtg ctagctggga tagcgatttt ctcgccagta
     4261 ttggtcagca gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtgaatc
     4321 cgcctgaatt attgcgctac gatgcgcaag gacgccgtct ggacgatgtg cgttttcacc
     4381 ccgcctggca cctgctgatg caggcgctat gtaccaatcg ggtgcacaat cttgcctggg
     4441 aagaagacgc tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatgcgc
     4501 aggttgaggc tgggtcgtta tgtccgataa cgatgacctt tgccgccacg ccattgttgt
     4561 tacagatgtt acccgcgccg tttcaggact ggatcacgcc gctgttgagc gatcgctacg
     4621 attctcactt attgccgggt gggcaaaaac gtggcttgtt gatcggcatg ggaatgacgg
     4681 aaaagcaggg cggttccgat gtcatgagca acaccacccg cgcagagcgt ctggacgatg
     4741 gttcttatct gctggtgggg cataaatggt ttttctcggt gccgcaaagc gatgcgcatc
     4801 tggtgctggc acaggccgcg ggcggtttgt cctgcttttt tgtgccgcgc tttttgcctg
     4861 acgggcaacg caacgcgatt cgcctcgagc ggctgaaaga taagctgggt aatcgctcta
     4921 acgccagctg cgaagtggag tttcaggatg ccattggctg gttgttgggg caggaagggg
     4981 aaggaattcg tctgatcctg aaaatgggtg ggatgacgcg ttttgattgc gccctgggta
     5041 gccatgccat gatgcgccgt gcattttcgc tggcgattta ccatgcacat caacgccatg
     5101 tttttggtaa tccattgatc caacagcctc ttatgcgtca tgtcttaagt cgcatggcac
     5161 ttcagcttga agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg gaccggcgcg
     5221 ccgatgccaa agaagctctg tgggcgcgtt tatttacgcc tgcggcgaaa tttgtgatct
     5281 gcaaacgtgg cattccgttt gtggccgaag cgatggaggt gctgggcggc attggttatt
     5341 gcgaggagag cgagctgcca cggctttacc gggagatgcc ggtaaacagt atttgggaag
     5401 gttccggcaa tattatgtgc ctggatgtgc tgcgcgttct caataagcaa gcgggcgtat
     5461 acgacttatt gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg
     5521 cggttcgtcg tttacagcag cagctgcgta agccagctga agaattgggg cgagagatta
     5581 ctcatcagct attcctgctg ggctgcggtg cgcaaatgtt gaaatatgcc tctccgccaa
     5641 tggcgcaggc gtggtgtaag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc
     5701 agatccagaa tgatttattg ctgcgggcga cgggaggagt gtgtttgtaa gcgtatacga
     5761 ctgatgcgac gctggtttcg attaactaaa tgaaatattt taaaattgta ggccggacaa
     5821 ggcgctcgcg ccgcatccgg cattgttcat ttgctgtaga tgacactcac gcatacagta
     5881 tcgcctgtac gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc
     5941 atgacgcacc ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc
     6001 ctgaaatatt gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac
     6061 tggcgaattc tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg
     6121 gtgaggcaag taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg
     6181 cattccatcg cggtaataaa cgcccgagct tccagccggt aatttattgt gcacaggtaa
     6241 acgcataggg acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg
     6301 tacaaaatgg cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca
     6361 acgtaataat cagcttttgc agcgacagct ttcgctctga tttctgctaa tgcgtcatcc
     6421 ggagaacccc gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg
     6481 cggcgaatct cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt
     6541 gcgctacagg cacttaacat cagcaccaat aataaactgg caaaccggta aataacgcta
     6601 ttacgtttcc tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc
     6661 cgaattgtta cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc
     6721 tcaacattat gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata
     6781 ccagacaaaa aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc
     6841 gttcttcatg cttatcctgt cgggcaaaaa gataccccgt taccgtgtgt gattttttat
     6901 cacggcttta cttcgtccag tctggtgtat agctattttg ccgttgcgct ggcgcaggct
     6961 ggtttgcggg tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgca
     7021 gcgcggcggt taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactacc
     7081 ttacgtgcgg caatagccga agaaaactgg ctgcgtgatg accgtctggc agtcggtggc
     7141 gcgtcgatgg gcgcgatgac ggcactggga attaccgctc gccaccccac ggtaaaatgt
     7201 accgccagca tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg
     7261 ctgatacctg aaacggcagc acagcagaat gaattcaata acattgtcgc gccgctggca
     7321 gagtgggaag cgacaaacca cctggaacaa cttggtgaca gacctctact gctgtggcat
     7381 ggccttgacg atgacgtcgt gcctgccgac gaatcactac gtttgcagca ggccttaagc
     7441 gagacggggc gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgcatt
     7501 acgcctgagg cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga
     7561 atgctgaccc cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg
     7621 atcagcatat cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta
     7681 ctgctatcaa ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt
     7741 gccgtcagca tatcggtttt atacaaccct tctgctgtca gtcctttgcc gctggtaaac
     7801 atccagtgcc cggcatagag actgttttcg ctgccttgcg ggcttaaagt gatggactga
     7861 cttttgttgt actgtccgcc cataatgatc acgctgtcat gctcctgatc gatcaggtaa
     7921 tttgccagcg gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca
     7981 agcagaaacg cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc
     8041 gaggccgctt tagcaatacg tactttttca tcgtgattct gcgcttgatg cagattcatc
     8101 ggcgtccagc gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ttttttcagt
     8161 ttgccgcttt cgtcaagttt attgatatcg cgtcgtgcag tggcaggcga aattcccaga
     8221 cgctcaacga ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt
     8281 tgatgtcttt gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata
     8341 tttgaaatag ccaggaaata ctatgccgga tagcgcgagc atctccacgc tatccggtac
     8401 aagcgacaga ttacaggaac gacttgaacg gcagatctgg ttcaatagtg aagcaatcat
     8461 cgaaaccacg cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc
     8521 cgccgacctg ccagataaac ggcttgaagc catacttcag gcgatctttt ttcatctccc
     8581 acagcacgcg gatctcttgc ggatcggcct ggaagtttga ccagatatcg tggtggaacg
     8641 ggatcactac tttcgcattc agcgcttcgc ccatacgcaa gatatcagca ctggtcattt
     8701 tgtcggtaat accgcgtggg ttttcgccgt atgaacctaa agccacgtcg atctgatgct
     8761 cattaccatg ttttgcatag tagttagagt agtgggaatc gccgctgtga tacagggtac
     8821 cgcctggggt tttgaacaga tagttcaccg cgcgatcgtc catgccgtcc ggcagtacgc
     8881 cagccgcttt ttgatcggct ggcagggtga tcagtgccgt gcggtcgaaa gcatcaagtg
     8941 catgaatttc aatgtctttc acttttacga catcgcccgg tttgaccacg atgcaacgct
     9001 ctttcggtac gccccagcca atccacaaat caacgcaggt tttcggtcca atgaacggta
     9061 cgtcatcagc acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt
     9121 gatcgtgagt cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg
     9181 gggtggtgcg caggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac
     9241 cctgtttcat gaacgggtta ccgtggcttt gtttgccagt accgcaccaa aaatcaacac
     9301 aaacgttggc accgccttct gatttcaacc agatcccggt acagccgagc caccacattg
     9361 cgaaagtgcc aggtgcgacc tgctcttgct caatttcttc attcagccag ctaccccact
     9421 ccgggaaagt gctcaggatc caggactcac gggtgatact tttcacttta ctcatcgcca
     9481 tttaccttcg tgatagttca atttgaatca acatgtgatt ggttttgatt aatcctgaca
     9541 ctattttttc aggaaggcaa tgaccatttt ttgacttttt agcaggagag ttattgtgga
     9601 ttttgagtgt ggaaagatta aattgaatgt gtaatgcaat taactaaatg aatttaaatg
     9661 aataaatatt tcattatgtg aatcccattc tgtttatgtg gaattatttg cgtgcgacgt
     9721 cacatttaat cataaataat cttgttgtga ttacttttga aaattagagt gagagcacaa
     9781 cattccgggt gtgcgaaata cccggttacc tcttcttcag gagatcgtta tggagatcct
     9841 ctacaacatc tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg
     9901 tattgtgacc tgtctgggtt acatcctact gcgcaaaagt gtcagcgtta ttattaaagg
     9961 tacgattaaa accataattg gtttcatgtt gttgcaggca gggtccggca tcctgaccag
    10021 caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc
    10081 cgatacttac gcgtcgatga tggcaaccat tgaacgcatg ggcgatgcct atagctgggt
    10141 gggttacgcg gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat
    10201 taccggtatt cgtaccatca tgctgactgg tcacatcatg ttccagcagg cggggctgat
    10261 tgccgttacg ctgtttatct tcggctactc catgtggacg accattatct gcaccgcgat
    10321 tctggtttcg ctctactggg gcattacctc caacatgatg tacaagccga cacaggaagt
    10381 gaccgacggc tgtggtttct ccattggtca ccagcaacag tttgcatcat ggattgccta
    10441 taaagttgcg ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg
    10501 ctggctgaat attttccacg acaacatcgt ctccacagcg attgtcatga ctatcttctt
    10561 tggtgccatt ctgctctcct ttggtatcga caccgtgcag gcgatggcag gcaaagtgaa
    10621 ctggacggtg tacatcctgc aaaccggttt ctcctttgcg gtggcgattt tcatcatcac
    10681 tcagggcgtg cgcatgtttg tggcggagct ctctgaagca ttcaacggta tctcccagcg
    10741 cctgatcccg ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgcgccgaa
    10801 cgccgtggtc tggggtttta tgtggggcac catcgggcaa ctgattgcgg ttggcatcct
    10861 ggtcgcctgt ggctcttcaa tcctgattat tccaggcttt atcccgatgt tcttctccaa
    10921 cgccactatc ggcgtgttcg ctaaccactt cggcggctgg cgcgcggcgc tgaagatttg
    10981 cctggtgatg gggatgatcg aaatctttgg ttgcgtctgg gcggtgaaac tcaccggtat
    11041 gagcgcctgg atgggcatgg cggactggtc aattctggca ccgccgatga tgcagggctt
    11101 cttctccatc ggtatcgcct ttatggccgt catcattgtc attgcactgg cttatatgtt
    11161 cttcgctggc cgcgcactgc gtgcagaaga agatgcagaa aaacaactgg cagaacaatc
    11221 tgcttaataa ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gtaacggaca
    11281 aggcagttcc atgatcatga aaatgaaagt ggaccagttt ttaacccagt cgaacatcga
    11341 ccacacggta aacagctgcg cggttggcga atacaaaagc gagttgagcg gtgcggatat
    11401 catcatcgct tctacccata ttgcgggcga aatcaccgtg accggcaaca aatatgtggt
    11461 tggcgtgcgc aacatgctct ctcctgctga ttttggcccg aaactgctgg aagtgatcaa
    11521 agagcatttc ccgcaggacg tgaagtaagg acgcgccatg aaattacgtg attcgctggc
    11581 tgaaaataaa tctatccgcc tgcaagcgga agcagagaca tggcaggacg cagtgaaaat
    11641 cggcgttgat ctgctggtag cggcagatgt ggtagagccg cgttactacc aggcgattct
    11701 ggatgcggtt gaacagcacg gcccgtactt tgtgctcgca cccggcctgg caatgccgca
    11761 cggacgcccg gaagaggggg ttaagaaaac tggttttgcg ctggtgacgc tgaaaaagcc
    11821 gctggagttc aaccacgaag ataacgatcc ggtggatatc ctcatcacca tggcggcggt
    11881 tgatgccaac actcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga
    11941 agagaatttc gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga
    12001 ccgcaccaac gcggcagctt aagaaggaat tgaacatgtc attaccgatg ttgcaagtcg
    12061 cgctggacaa ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag
    12121 tcgacattat cgaagtgggc accattcttt gcgtaggcga aggcgtgcgt gcggttcgtg
    12181 acctgaaagc gctgtacccg cacaaaatcg tactggctga cgccaaaatt gccgatgcag
    12241 gcaaaatcct ttctcgcatg tgcttcgaag ccaacgctga ctgggtgacc gtaatttgct
    12301 gtgcggatat caacaccgcc aaaggcgcgc tggacgtggc aaaagagttt aatggcgacg
    12361 tgcagatcga attgaccggc tactggacct gggaacaggc gcagcagtgg cgcgacgcag
    12421 gcattgggca ggtggtttat caccgcagcc gtgacgcgca ggccgcaggc gtggcgtggg
    12481 gcgaagctga cattaccgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca
    12541 ccggtggtct ggcgctggaa gatctgccgt tgtttaaagg cattccgatt catgtgttta
    12601 tcgctggtcg cagcattcgt gatgccgctt ctccggtgga ggccgcacgt cagttcaaac
    12661 gttccatcgc tgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg
    12721 gcatctacga aaaagcgctc cccgctgggg agtgctggct ggaacgactg caactggcaa
    12781 aaacgttagg cttcgatttt gtcgagatgt cagtagatga aacggacgat cgcctttcgc
    12841 gcctcaactg gagccgcgag cagcgtctgg cactggtgaa tgcgattgtt gaaaccggcg
    12901 tgcgcgtacc gtccatgtgc ctttctgctc atcgtcgttt cccgctggga agcgaagatg
    12961 acgcagtgcg ggcccagggg ctggagatta tgcgtaaagc tatccagttc gcccaggacg
    13021 tcggtattcg cgtgatccaa ctggcgggct atgacgttta ctatcaggaa gccaataacg
    13081 aaacgcgtcg tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc
    13141 aggtcacact ggcgatggag atcatggatt acccattgat gaactccatc agcaaggcgc
    13201 tgggctacgc acactatctc aacaatccgt ggttccagct ctacccggat atcggcaacc
    13261 tgtcggcgtg ggataatgac gtgcagatgg agttgcaggc tggaatcggg cacatcgtcg
    13321 cggtgcatgt gaaagacacc aaaccaggcg tcttcaaaaa cgtgccgttt ggcgaaggcg
    13381 tagtggattt cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc
    13441 tgattgagat gtggagcgaa acggcggaag acccggcggc agaagtagcg aaagcgcgtg
    13501 attgggtgaa agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct
    13561 gaaacagcag gtgtttgaag ccaacatgga tttaccgcat tacgggttgg tgacctttac
    13621 ctggggcaac gtcagcgcta ttgaccgtga acgcgggctg gtggtgatca agcccagcgg
    13681 tgtcgcctac gaaaccatga aagcggacga tatggtagtg gtcgatatga gcggcaacgt
    13741 ggtggaaggg gagtatcgtc cgtcttccga cactgcgacg catctcgaac tctaccgtcg
    13801 ttatccgtcg cttggcggca ttgtccatac ccactccact catgccaccg catgggcgca
    13861 ggcggggctg gcgatcccgg cgttaggcac cacgcacgcc gattacttct ttggcgacat
    13921 cccgtgcacg cgcggattaa gcgaagaaga ggtgcagggt gagtacgaac tgaataccgg
    13981 caaagtgatt atcgaaacgc tgggtgacgc cgagccgctg catacaccgg ggattgtggt
    14041 gtaccagcat ggaccatttg cctgggggaa agatgcccac gatgcggtgc ataacgcggt
    14101 ggtgatggaa gaagtggcga aaatggcgtg gattgcgcgc agtattaacc cacaactgaa
    14161 tcacatcgac agcttcctga tgaataaaca cttcatgcgt aagcacggtc ccaacgccta
    14221 ttacgggcag aagtaaaaca cgcgctgcgg aaatttcctt cttcgggaga taactggtct
    14281 aattccgcag ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca
    14341 atgtttcgtt ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc
    14401 gttactcccc ggttcccgca tcagaatcac atggtagtac gttgcgcctt gcgcatctgt
    14461 ctcttcattt aatgcctgac gtgcttcact ttcagtggcg aagttatggt tgatataaat
    14521 cacgcctaag ctttgcacat catccatatt tctggcctgg tggttattaa tttcaatggc
    14581 tgcccatgta tttgcactta gcaaaagcac agccagaagg gctaaaacac gactgaacat
    14641 agatacctcc tcgacggctg actttgtgtg ctctcctctt gtggtgatct tctgatttaa
    14701 ttttaatcaa tgataaagaa gttgatggtg accatttctg atgcagttgt tcaaaaaaac
    14761 accatgatga agtgtgatga actccaaatc agcgatttag aggttatttg cgaaagggga
    14821 gatttatttc ggctctgccc ttgagtttag cggggtatgt cagtactata acgcgtcatt
    14881 tttcagccga cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag
    14941 gctgaataat ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat
    15001 cctgaccaga gcgaacaggt tccgggcatg atcgagcgct acactgctgc catcactggt
    15061 gcagaaggca agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc
    15121 aacaaactgc acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc
    15181 gatgagctgg aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt
    15241 accaagcacg ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag
    15301 cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag
    15361 taatttctga tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga
    15421 aaggtcagtc catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag
    15481 gaggaagccg gctttcaccg gcaggcgtgg tgccaaatgc ccgttattgt tagcggacac
    15541 gaaaaccagg ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc
    15601 atttcatgcc ataaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt
    15661 gaattgatag attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg
    15721 ccgtttcacc gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa
    15781 ctacatcacc gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata
    15841 ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac
    15901 tgatcgccat cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg
    15961 caagttattc tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt
    16021 aaagcgggct atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag
    16081 aaaaacattg aattcttcga agcacgtcgc gctgaactgg aagctaaact ggctgaagtt
    16141 ctggcagcag ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg
    16201 tctaaagctg gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac
    16261 gctgtaactg cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt
    16321 ctgcgtacca ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa
    16381 gtgatcgtaa acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc
    16441 agtggtcggc gttttgcttt taagcgataa aaaccgcaga aatagacgtt ccctccataa
    16501 atctcttgct attcctttta taaaaatttc gcagtaccca tacctggccc ccagcctcgc
    16561 cgctggtagt tcgtgtcatg ttttttacgc gtaaaacacg acaaaagcat caatacagcg
    16621 atcgtctccc ttgtatgata ataaaaacaa tatgttatat atgaatgcgc tgttatttta
    16681 ttgtttttat atataaaagg ataaaatatc atcactttcg taatcttgaa cgctttgcag
    16741 gtgcgttcaa tccaataaat gctaatatta tggctattat agccataaaa agataaaata
    16801 ttatcactat gggtaaaaaa acagcaccgt tactccctta tacgaatcag atgcttagtg
    16861 aatttggtga gcggctgaaa cttgctcgtt tacgacgcag gcttaccgca aagcaagttg
    16921 ctgaacgtgc tgggatgtcg ccaataacat tgcgctctct ggagtcagga agtgcagggg
    16981 tcacgatagg ggcatatttg tctgtgatgc aggttttggg cctggaaacg gatttaaata
    17041 agcttgcagc tgaagacgac ttagggcgtc agctccagga ttcccgtttg attaaaaaaa
    17101 gagggctttc tcgttcatca ggtaaaaatg aagaaggtat tcagacagac ccttttcaaa
    17161 cgaggtatga gaaagaaggt ttactcacca cggatttaac agcactggca gaaggctcaa
    17221 cgccatctat tacaggagaa acatcaacct ctcttgcagc actattaaaa ccgataaaaa
    17281 aaccgttaaa aggaaattaa ttatggcatt aatcggagtg tatgcggatt gggaaggcct
    17341 ggatggtccg gagcggattg gttatttaca cagtcgccgc actcggacgc gtgagatatt
    17401 tgagtttgag tacgataaaa aggcgcttgc agatccttca cttaatttca ttcagttaga
    17461 tcctgaaata atgttgtatg agggagcaca gtacccaatt cctccaaagg ataaatttgg
    17521 tgcatttagt gattcctgcc cggatcgttg ggggcgtatg ctaatgaagc gtcgttttga
    17581 gagggatatt cgagacgggt tatgtgataa agactcccac ctttatgagt ctgattatct
    17641 actaggtgtt catgatcttt atcgggttgg ggctcttcga tacaaacgtg aagacgctgg
    17701 agagtttctt gataatcgca ttgatgttgc agccccacca tttaccgaaa tagcgagtct
    17761 ggaaagggct agccgggcaa ttgaagaaga tcctgataat aaagagctaa tggggcaaaa
    17821 atggttaaga atgctaatcg ctccgggggg atcattaggg ggagcacgtc cgaaaaccag
    17881 tgttgttgat gaagctgggc atctctacat cgctaaattt cccagtgtaa aagatgagta
    17941 tgatgttggt ggatgggaaa tggtcgttaa tgcacttgct gttggctgtg gactgaatgt
    18001 tgcacctgct caggcgcata aatttgccag caattaccat tgttttatgg ttcgtcgttt
    18061 tgaccgcact aatgcgggaa ggcgtctgca ttttgcttcg gctatgacac taaccaggca
    18121 tcaggatggt gaagatgcct ctacaggtgt gagttatctg gaattagcag atgttcttat
    18181 caggcatggc gcgcagacta atactgatct taaggaactc tggtccagga ttgtttttaa
    18241 tattcttgtt tctaataccg atgaccattt acgtaatcat ggatatatct tgataccggg
    18301 aaaaggttgg cggctttctg aggcatatga tttgaatcct gttgccaggt ctgacggtct
    18361 aaaactcaat attacagaaa atgataatgc acttgatttg gaattagctc gagaagttgc
    18421 ggagtatttt cgacttggtt tgacagaagc agatgacatt attgctaatt ttagagggat
    18481 tgtcagtcag tggcgaatca ttgccgagcg tcttcgatta cctggtcgtg agcaagagtt
    18541 aatggcagag gcgttcaggg gggcaattta gaatagttat ttcgatatat tcgctaaatc
    18601 ataaatttgc gaagcaatgc cagtagatac tgaacgtttt ggaagagacg tttaatttat
    18661 gagaaaaata gaaacgctac aaagctaaat tatagcgttt ctatgtaaaa taattagctt
    18721 gttgctaact gtacttgtgg ttcttcgtct ttaactttga taagcgtcca cagcgcaatg
    18781 gcgccaataa tgtcaaagaa agccagggca ataaagaacg gcgcgaagcc aataatgtga
    18841 acgaatgccc cgataaacag gttgaaaatc aactgaccgc tccatgcaca ggctcctgcc
    18901 atacccacag cggtagctac ttcgtctttt ttgaataggt caccgcccag agttgctgca
    18961 acagtagaaa gacattgatg cgagaatgcg ccaatgctaa tcagtaatac ggcgatgtag
    19021 ggattttcga caatactcac cagaccaatc gtcatcataa tgacggctgc aatagtgaag
    19081 gtaattcttc gtgagttaat taaactcacg ccgcgatcgt ggaagaactt cgcgacaaaa
    19141 ccactggcta aacagccgag atcgcccagc aggaaaggca gccagacgaa catggcaatt
    19201 tctttcaggc taaaatgcag cgtttcgacg aagaaaatcg gtacccagaa gttaatggtt
    19261 ccccatgccg ggtcagcgag aaaacgcgcg atgccaatcc cccagaaatt acgttggctg
    19321 aggatatgct tgattgacgt tttttctttc ttatctgttt taagatgctg ttcttgccca
    19381 gactcaatat agtggcgctc ttcatcagaa aggcgtttag catcttttgg atctttataa
    19441 caaaagaacc aaaataaagc cgcgagtaaa gcaaggctac ctgagacaat aaacgcaaat
    19501 tgccagctat gaaacataat gcaccaggca atcaacggtg gagcaagcat cgcgccgagt
    19561 gaggtgccca tattgaaaac acctgtcgcc acgccacgct cttttgccgg gaaccatgtt
    19621 gatgcggttt ttacacccgc aggaattgcc gatgcttcgc tgaaacccat cagggcgcgc
    19681 ataaatgcca gacctgacca tgttcctgtc aaggcatgcc ccattgtcgc caggccccag
    19741 attgcggcac atatcgcaaa acttaacttc agacccacgg tatcaataaa gaagcccatt
    19801 atcggttgcc ccaatgtata ggccaactgg aatgcactga caatccatga atattgttct
    19861 ttggttattc cggttgtctc cataatcgtt ggagccgcaa tacctaacgc cgttctgtcc
    19921 agataattga ttacagtgat aaagcatacg agggcaatca tatgccatcg ataattcttt
    19981 attttattca tttttaaacc ttttaattat attaataaac cctggttgtt ggtatttcca
    20041 tgtaataggc aataaaaatc aggtacagag gaaatattgg aaattattgt gtgtaaatgt
    20101 ctccctttaa aaagtctggc cagttttttt caaagtaaat accgttaata cacggttgaa
    20161 tacgtaaaga ctttacttcg gtaataaaag aaccttgcga atattgaatt gttaatttgt
    20221 gatttttgtc ttcagcgagt tcagagcgaa aacgccattc ttcttttgcg ccgcgacgag
    20281 tttgtggtag gcatttgcct tgtggatcaa taaccatacg cgccccacgg ctaccaccac
    20341 cagcatcaat ataatgcgtt aattgtgtta ataccgcggc agaagtcagc gccatatgcc
    20401 gccacataaa cagctcggca acttcaccca catgtttgat tgccaatcca tgccgttgca
    20461 caaattcact caatagcagg gcatcacgag tggcgcgtcg gactttatcg gcatggcaaa
    20521 taaatcccgc atggtcagac attcgtgcct gaattttttc cctcacaacc gacaacggca
    20581 tcccggttcc gttatcgtgc gcctgagtaa tgatctctct tatagaagcc agtgccggag
    20641 ctgccagctg tgcaatatct ccatcaatat tccgcttttg cgtgcaacca ataaagcgcg
    20701 ccagacgaac agcaaaaacc tgcccggcat tcaatgccgc accgccaggt cgggtgacgc
    20761 cgtgtgtgcc agcaacttcc cccaccgcaa aacaaccggg cagagatgtt tgtccccaga
    20821 tatcgacttc aatgccgcca ttcatgtgct gattattcat ggcaaactgc aatggctgcg
    20881 tgatgagatc gtaaccgtgc atcttataca gcgagataga cagaggattc atttgttgca
    20941 ggcgttcgat gggcgatggt gccagagcgt cgttattttc cagatacgcg cgaacgtcgt
    21001 cgtccagtcg atctaatgag aacggcaggt cacccggcac aggttctgga ttacgattga
    21061 aatccataaa tacctgacga ccatgttgct gctcttgcgc tactgccata tctaacaagc
    21121 tggagccaaa atccattacc cgcgtggcgt ggaacggcca ctgatagcct ttacggaaaa
    21181 tgtttgaagc cagctcctgg gtggtgcgat agtaatccgc gaggaagtta tactcgttac
    21241 ccccggcatc cacggaatag atatatggga tcacctgtac ataagtcccg gacaaattcc
    21301 acggaaacgt gctgcgcggt gtgccgatac caaactggct ttcagtcaga ttggttagcg
    21361 tcagaccttc ctccagcgcc agccccagcg agccgaaaca tttgtgtgga tacacactgt
    21421 cgcgataaag ctcgccaggc ccgcctgttg ccagtaccac attgggcgca gtcacaattg
    21481 ccagccccca tgggttatgg gcgcgatgac cggtcgcgag gattgccccc gccacgcggt
    21541 cttcgccgtt ttcgtcacgc tgatgcagca gtttaatcac tgttgcactg gtcaacattg
    21601 ggatagcaag acgctgtact tcttccagca gcactttcac cattaacctt gaggtacgcg
    21661 gcccacacga ggttgcacgc ccggcttcgt cgtggtcggt ttgataacga agaatcgcgc
    21721 catagcgatc ttccggtaat tccagaccaa gatattgcaa cccgccaaga gtgtgcagag
    21781 aacctaccgc ttcaacataa gcggtgtcgt gatccatcgc cccaccgctc gccagtgcct
    21841 ctgccagttt ggcgaagttg tcgccgttgc ccgcggtagc ggcggtaaac agtgtttgtt
    21901 tatcagagcc agaacatgcc gatgtcccca tatataaccc ggcggtggcg attaacacat
    21961 tttgctggcg gcgtttaagt tccacagctg cacgtagccc ggctgcacca ctgccgacta
    22021 ccagggcatc tgttttccag agtgggtaat caacaccgtc taccgtgaca acccatgcgg
    22081 gttctgcctg tgcaaaaccc tttgggaaag taacgtgtgt cagtgcatca aggatggcct
    22141 catcaagctg actcatgcat cctccattgg cgtaaacagg ctactttcca tcaggcgtgg
    22201 gtgcctgata atcggacgga aggccatata cggcagaatg tcgcggtcaa tctctacgcc
    22261 aggggcgatc tcaattaatt caacgccttc tgccgttaac tggaataccg cacgttcggt
    22321 gatatacagc acttcctgac tgctttgctg tgcgtatgcg gcgctaaaag taattttttc
    22381 cacctgggta accagtttgg ggatttgacc tgactgggcg atatgcaggc catcaggcgt
    22441 tacatcaatc ttgctgcctt tggcgtcaaa cgtaccgcag aacaccactt tacgggcgtt
    22501 ttgcgcaatt tcgaggaagc cgccggggcc aatgagattg ccattgagat gggatacgtt
    22561 gacgttaccg taccgatcca tctctcccat gccgaggaag gtgatatcga taccgccgcc
    22621 actgtagaat tcgaattgat cgagtgatgg aataatggca tcggcgttac gggcgcagcc
    22681 gaacagagca tcatccagca tcatgccgtt atgtacaccc tgttctacac tcagccacaa
    22741 actttgataa ggcacgcctt cgcgcagtgc aaccccagga ataccgccgg gaataccaaa
    22801 gccaaagttg gttgaagcgc ctgccaccag ttcccgcgct gcccgacggg caatcaggcg
    22861 acgcaccgga tgactaacca gttcaatagc gtcgttagaa cttagacggc gatgttgacc
    22921 gctgatggtc aggtcgtaac cgccaagata ggtttgcggt tgttcgcggt gctcaacgat
    22981 gccatcgacc agaattcctg gtagtttgac ccgacgtggt tcaatggtac ctgcttccag
    23041 cacatcgcgg acctgtacga acactttgcc gccgctgttg tgtgctgcca gcgccatcga
    23101 atagctatcc atatcaatcg cctcttcttc gaggctgacg ttgccacgcg gatcggcata
    23161 ggtgccgcgc aaaatcgcgt agtccacttt gaaagggcga taacgaagtt tggtctcacc
    23221 gtcgatttca atcagttcta ccaggtcgtc ggtagtgcat tcattcgact taccgccacc
    23281 attgcgtgga tcaacaaacg atcccagccc aacgtgggta aaaagccccg gacgtccggc
    23341 accgatctcc cgcagcagtg cctgaattac gccaccaggg aaacagtagg cttcaatagc
    23401 attgcttttc accagttcct gcatcttggg cgaccaggta aaatgtccgg caataatgcg
    23461 tttgagcatt tcggcgtgag caaaacggtt agtgcctttg ctgtcgcgat cgccaatacc
    23521 cagcgagtgg attaatgtca gatcgcgcgg gtgtccggtt tgcagaaagc gcgcttcaat
    23581 ggcggccaga atatggtcag cttccaccat gccgccgccg ttaccgctaa tggcaatggt
    23641 tgcgccgtcc tgaatttgcg ccgccagtgt ctcggcggtt gataacttac gcatacaatt
    23701 tccttattga ttgtggaagc tcacgccggc tttttcggca aatgcgcgcg tcgcctgctg
    23761 gcaatcctga gtggcggaca ttaatccagc cagcaactga tgcgagtaat cggcgtgttt
    23821 attttccagc gtcagaatca gttgtttaat gtgtgccagt gccaccggac cacatttttc
    23881 cagctgcgct aactggttcg ccatccagct ttccacctgt tctttttcta ccacttcatc
    23941 aattaaattc gctgtctgag cttcctgcgc cgtcaggcgt ttacccagca tcagcatctg
    24001 gcgaccgacc acaggaccaa cctgattaag cactcgctca atgcccatcc agcctggcac
    24061 catgccgagc atcacttccg ggttagagaa tttcgcgccg gggcgagcga tacgaatatc
    24121 gcagcacaag gcaagttcaa taccgccacc gatggtatga ccgttgagat tcgcaacggt
    24181 taattggggt aagttgcgca ggcgattaaa aacgtcatta ccgcgtttaa tccattttct
    24241 gcccatatct aatggcgaat aggccgacca cgatttaata tcgccgcccg cacaaaagaa
    24301 tttatcgccg gttgccgtta attcgactaa tcgtaatgtg gtgtcgtttt caatttcatt
    24361 taaataatgg tcgagagatt cgatcatttc ttcattaata gcatgacatt tatcttcacg
    24421 gttcaatgtc agacggcaag aagccgccgc tctgctgaac agaacgggtt ggtcactcat
    24481 gattattcct taacaataat gtgcgatgcg atcgacgcta atatccagcg tgctgttaat
    24541 atctttctgc caccagtacg gtgagaatat ttccagttca atagcgccat taaatccggc
    24601 gttttcaacc agccgacgaa ttgacggaat attgatgaca ccatctcccg gcattccacg
    24661 gtcattgacc agatcggtgg tcggcactaa ccagtcggaa acatgaaacg cgaggatgcg
    24721 ttttccggca cgcaggatct gactggcgag atccgggtcc caccagacat ggtagacatc
    24781 taccgccacg ccgaggccaa attcgccatc gggatcgagt tcatcacacc agtccagcgc
    24841 ctgacgtaac gtgcacaaac aggaacgatc ggctgcggtc atcgggtgta gcggctccag
    24901 cgcaattggc acaccgacct ctttcgaatg tggcagcaac tggcgaatcc cttgtttcac
    24961 ctgttcccgc gcttcgtaca gatcttttgt tcccattggc agaccgccaa ccacctgcat
    25021 ataacaggcg gcgttcagta cagcggcgtc gtccagcgcc cggcggttat cgtcaatcgc
    25081 cagtttgcgc tccgccagcg tcggtgcggt gtagtaggtg ctacggcaca aaccggaaac
    25141 gttcatgtta ctggcagcaa gctggcgggc gatttgctgg agatcttcac tctgcaactc
    25201 cctgcgccag ggggcaatcg cgccaatacc gcgtgcggcg caggcatcaa taatggcggg
    25261 tagcggcgtt ttatagccca gtgtcgcggt attgatactg aataaatccg ggcgttgtaa
    25321 cagatctctc atgattcagc ctcagaagcg cggcatactt aagccaccgt caatggcgac
    25381 cgcctgacca caggtgtaaa taagtttacc ttccgccatc gcgcggacag tggaagcaat
    25441 atccgccgga taaccccaac gtccccacgg caccagacct ttggcaatca gttcgtcgta
    25501 ataggccgtt gccggaatag tcatatcggt tttgatgaga ccaggacgca cttcatatac
    25561 gccaatttgt tcattacaaa ggcgagcagc aaacagacga gcagcggcag agacggcagt
    25621 tttggcaatg gtatattcgc cgcgattcat cgccagcatg atggcgttga tggaactgac
    25681 aaaaataatc gagcggtgcg gcagttctgc ttccggtttc ggctgtgcca gtaagcgctt
    25741 actgaatgcc tgagcaagga agaacggtgc gcgggtgtta atggcaatat tttggtcgaa
    25801 gctgtctggt tcgaggtcga gaaggtcacc gcgttttttc actgaaatgc ctgcgttgtt
    25861 gagcaggcag tccagacgtc cccactggtt ttgcgctgcg tcgagcatct cttcatgcag
    25921 ggagagatcg cccacatcag cagggaagca aatcacttcc acgccttccg ccagacattc
    25981 ttgttgtgtg gcatgtaatt tttctacgct atccgcatcc ggacgatcat taatcagcag
    26041 attaaatccg ccacgcgcaa gctccagcgc acagcccttt ccaataccac gtgcggctcc
    26101 ggtaatcacg gcaacaggac gagtggattt catacaatag ctctcttttt ttgaattttg
    26161 tttccttagt gatgattagc ttgcgactgg aggataaaaa gatcaaccat ctagttggtt
    26221 gatatgcgat ggcgatcaca ggacatttga taacctgatg aaagtacaag tttttgccct
    26281 tttttctgaa gctgaaatca aatcaggtta tgatagtcac cacgaagagg tcgtggggaa
    26341 gcgttatgta tcttgatacc agcaacgtac agagcctgaa agaaaagctg ctgttatgcg
    26401 cggttaatga atttgccgaa tatggttacg aaggggccag agtcgataat atcgtcaagg
    26461 ccgcggggtg cagtaaacag acggtttatc accactttgg taataaagaa aatttattta
    26521 tcgaagtact ggaatatacc tggaacgata ttcgacaaaa agaaaaggcc cttgatttct
    26581 cagatcttcc gccacagaaa gccattgaga aaataatcga ttttacatgg gattattata
    26641 tttctaatcc ctggttttta aaaattgtgc atagtgaaaa tcaaagtaag ggtgtgcatt
    26701 atgcaaagtc acagcgtctt ttagaaatta accatgcgca cttgcagtta atggaatcct
    26761 tattggatga agggaagaaa aacaatattt ttaagccaga tatcgaccca ttgcaggtga
    26821 atattaatat tgctgcgctt gggggatatt atttgatcaa ccagcatacg cttggtctgg
    26881 tttatcacat cagtatggtt tcaccgcagg cactggaagc cagacgtaag gtcatcaaag
    26941 agacgatcct tagctggctt ttggttgacc cttcatctac cgcgcgcgaa taaaactgcc
    27001 atccggctgg cgggtaaaca ggacctgttg attattcccc gtatcaatgg ttaagcccgt
    27061 caccacgccg ctggcgttct ggcggatttt caccatctgc ccgttttgca aattactcag
    27121 tggcttatcc gcgccttcta cctgcgccat ggcatagaca tcggtagcgg gcagtccgtg
    27181 gtcgcggaac atctgcgcca tcgttttacc gggttcgaca cgataggagc gccattgatt
    27241 atcaattccg ctatctggct ggaatggttg cgtatgggtg gtttgcggct gctcttctgg
    27301 ttggccttcc tgtattggtt ctggagcgac cggagccacc tgatcaggat cattttgtgg
    27361 cgtgactaat tgcgcccgca gctgttcttc tgtcggtggt tgtgattgcg actgaatatc
    27421 taactgcgct tcacgcgtca ccaccggcgc gttgggcgta tcatcagatg gaagcagaaa
    27481 tccgaccacc aatacaatgg cggcaataat gatcccccga cgatgcatcg gcggcagcgg
    27541 gtccatgaag cgaaaattgt ccggcgcgtg ccagactttc tccagggttg gttttagttc
    27601 aaagcgcccg ggcatggttt cctcctgctc cgcgtcttgt tcctcaatca tagcctatga
    27661 ataagctaac gctatgatgt ccgtggtaaa cccgccttta ttatattcat acgggatatt
    27721 gctattgttt ctttttcccc gggatttgtc atcattcccg cgacaaagtt ttacccaaag
    27781 aagtgtggct gatatgctgc ccgctacttt ataccctaag aaaggaaata cgatgaccac
    27841 cccaactttt gacaccatcg aagcgcaagc aagctacggc attggtttgc aggtagggca
    27901 acaactgagt gaatctggcc tggaagggct gctgccagaa gcactggttg caggcattgc
    27961 cgatgcgctg gaaggcaaac atccggctgt tccggttgat gtggtgcacc gcgcgctgcg
    28021 tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt ttccagacga tggctgctga
    28081 aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt gtaaatagca ccgaatctgg
    28141 cctgcaattc cgcgtgatca accagggcga aggcgcaatt ccggcacgta ccgaccgcgt
    28201 tcgtgttcat tacaccggaa aactgatcga cggcaccgtg tttgacagct ccgttgctcg
    28261 cggcgagcca gctgaattcc cggttaacgg cgtgatccct ggctggattg aagcactgac
    28321 tctgatgccg gtaggttcta aatgggaact gactatcccg caggaactgg catatggcga
    28381 gcgtggcgca ggcgcgtcca tccctccgtt cagcaccctg gtgtttgaag tcgaactgct
    28441 ggaaatcctc taagcagcgc attctgttcc cctcgagcca gaggggagca ggcattcagc
    28501 aataaaccct tcagtttgcc aaacggcgct attttatgtt gcaaagaccc tgtaagcgtg
    28561 tatttttgtg agctgtttcg cgttatcacc gtgatatgac actcacttta aacataaaat
    28621 taacattaga tctaaatctt agtattcatc ccgcgtattg ttacctaata tcgatgagtc
    28681 ccgatacaga ttcgtcgtat catagactga ctaaaggccg tagagcctga acaacacaga
    28741 caggtacagg aagaaaaaaa catggtagat caggtaaaag tcgttgccga tgatcaggct
    28801 ccggctgaac agtcgctacg gcgcaatctc acaaaccgac atattcagct tattgccatt
    28861 ggcggtgcca ttggtacagg gttgtttatg gggtccggca aaacgattag ccttgccggg
    28921 ccgtcgatca ttttcgttta tatgatcatc ggttttatgc tctttttcgt gatgcgggca
    28981 atgggggaat tgctgctttc gaatctggaa tacaaatctt ttagtgactt cgcttccgat
    29041 ttactcgggc cgtgggcagg atatttcacc ggctggactt actggttctg ctgggttgta
    29101 accggtatgg cagacgtggt tgccattacc gcctatgcgc aattctggtt ccctgggctt
    29161 tctgactggg ttgcttcgtt atccgtgatc attctgttac tggttctaaa cctcgccacg
    29221 gtaaaaatgt tcggtgagat ggagttctgg tttgcgatga tcaaaatcgt cgccatagtg
    29281 tcgctgattg ttgtcggcct ggtcatggtg gcgatgcact ttcagtcacc gaccggtgtg
    29341 gaagcgtcat ttgcacattt gtggaatgac ggcggctggt tcccgaaagg cttaagtggc
    29401 ttctttgctg gattccagat agcggttttc gctttcgtgg ggattgagct ggtaggtaca
    29461 acagctgcgg aaaccaaaga tccagagaaa tcactgccac gcgcgattaa ctccattccg
    29521 atccgtatca ttatgttcta cgtcttctcg ctgattgtga ttatgtccgt gacgccgtgg
    29581 agttcggtag tcccggagaa aagcccgttc gttgaactgt ttgtgttggt aggtttgcct
    29641 gcggctgcca gcgtgatcaa ctttgtggtg ctgacctctg cggcgtcttc cgctaacagc
    29701 ggtgtcttct ctaccagccg tatgctgttt ggcctggccc aggaaggtgt ggcaccgaaa
    29761 gcgttcgcta aactctctaa gcgcgcagta cccgcgaaag ggctgacctt ctcttgtatc
    29821 tgtctgctcg gcggcgtggt gatgttgtat gtgaatccca gcgtgattgg cgcgttcacg
    29881 atgattacaa ccgtttccgc gattctgttt atgtttgtct ggacgattat cctttgctcg
    29941 tacctggtgt atcgcaaaca gcgtcctcat ctgcatgaga agtcgatcta caagatgcca
    30001 ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg tgtttgttct ggtgttgttg
    30061 acactggaag atgacacccg ccaggcgctg ctggttaccc cgctgtggtt tatcgcgctg
    30121 gggctgggct ggctgtttat tggtaaaaaa cgcatggcga agtaatgccg cgtttataac
    30181 tctgaaaggc acctgcgggt gcctttgtct ttatatattc ctaaaaaaat ctaccatcat
    30241 agacagccct caggcgcgag cttaccgtgg atctttgcca ctcgttgcag agttcattat
    30301 ggttaagcgc agttggttat taatcgccgc attacctctc tctatttcac cttcctgggg
    30361 cgcggatttt tattaccgcc agcaggagaa aggcacggtt tatgttgtcg aacagaaggg
    30421 ggaaaaagat gagatcctct ccgaattacc agatattaat ttttcccgcc tttggcgcat
    30481 tgccaattta gccaataaac aagattcccg gttactgtcc gattttcccg ataagttcga
    30541 ttgcgatgat gagggggatt gcgaacatgt ctggctcacc gacggacgct ctgttctttg
    30601 gtctggcaaa gtcctgaaaa atccccccgg taaacctaaa gtcgacgctg ccagttttca
    30661 ggcattcggc gctttcgctg ctgataaacg cagtatctat tttgacggtc agcgtaccga
    30721 tgatagatga agtggatatg tatatagctg tgtctcagtt gcaaacaaat atttatgtac
    30781 cgaaataaaa gtatcaatag acaattaatt aataaatcag gagataaagt ggctatatta
    30841 catccacagg agtgctggtt gctggaaaga ataatgtcgc cggaatatta tcggcgtcgt
    30901 tttgagggct ggcaggagtt tgtcgagctg tgcgagcgac aggtggcaga gtggagcaag
    30961 accatgccgc tggatgtgcg ccgtcgtccg ctgtgcgagc agattgacgc cgtctggggc
    31021 gggcgagtgt tgcccaatat ccgcagtact ttaaagtcgg tacagtatga ctttattcag
    31081 ctacagcagg gggacctgcg ggtactgcaa tccgggggta acattagcag tgatatgaaa
    31141 ggactaattg attatccgtc ggactggatg tcactcgtcg cgcaaaaaca gtatgacaga
    31201 ctgaaatggc gaggtgcaca ttataataat ttaatcagac gtacctcggg cggatactgg
    31261 tatgacggcg aactgactta ttattatgag gaaagtctgc acggcccgct ggccttgccg
    31321 atgcaactac cgttgtatga gctggatagc cgcgtatacc tgcgtgagga tgacccggta
    31381 acgttggctg gcttgtatct gccggatatc ccagatgcca gcgcccaact gttgtaccgg
    31441 agtgaacata ttccagaggc ttggcagggg cgggtacgaa cgaagtatgt caatgaggct
    31501 gggatacaag aatattactg ggaaaacggg gcgtgggaaa aatgcaactg gatacgtata
    31561 cggcgggtgg caaaccgatt tatcgatgtg ccgccggagg gatttttccc acagggcatg
    31621 ccggaggagt tgtacaactg gccacagcgc gaggcgcagt atgtaacaga ccggcagcgg
    31681 atggcggcct gtagcggtga agcttgtcct catagcgggg agtggtcgat ttttgtagaa
    31741 ggtagacaag cgacggtgac actggagcag ggagagcaga tgccagaatg gacggacagg
    31801 aaaatggagg gggagtataa aagaggcgag aaattccatg tgctgtggtc gctgatgaac
    31861 cgccatgatg gcgggtcggt gtgggtggaa gcgtagcggt agctggatgt acacgtaaaa
    31921 accagggaat gggtgatttt attctggctt atacaggagc gatgaataaa taattatatt
    31981 aatgaaaaaa ataagttagt aaccgttgaa ggaactaatt attggctgaa taatgaattt
    32041 tcccagtcca gtagatacaa aagattgtcc cggagtggtg aataactcaa agcatttgaa
    32101 aacgcacctg attgtgtaaa ccaggtgcgt tggtcgaaca aatcactcac ccgccagcgc
    32161 gcgcgggaat agtacattgt tttccagact gatgtgctcc atcaggtcat caatcagttc
    32221 attaatgccg ttatacatcg ctttccaggt ggtgcaggct tccggcggcg gtgtgacgtt
    32281 attggtggtg tgtttgatca cttccagcag ttcgcccgct tcatcgtgct cgctttccat
    32341 tacgctgatt ggccccattg cctggctgcc catgccttgt ttaatcatcg ggaagagaat
    32401 ttgctcttct ttcatcatgt ggctggaaag ctcttcatgc agcatagtca ggtattttgt
    32461 cagccctttt ggcacacttg gtttgtcggc gtgaacgcgc tcgactttag tcgcttgcag
    32521 gatcagctcc ggcaactgct cgcgatggcg atcgtgatag cgcacaataa tgtggtcgat
    32581 gatttccgcc agcggcgcgc tacgccagtc tttctcaatc ggttgttcag caagctttgc
    32641 cagttcggct tcaatgacgt caacatccag ttctttacgt gccgccgcac gcgccagcgt
    32701 ttgcttaccg ccgcagcagt aatccatatc atatttacga aacagagccg aagcgcgagg
    32761 aatagagagc gccagttcac ctaaaggttg gtcgcgataa gccatagctg atacctcatt
    32821 cttaataaga taagatgcat tttaaatgca tctttaaggc aaaagctata acccttacgt
    32881 agcagaggct tatttaactc actgccagcg tgtcaacttt ctgagacaga ttcagttaaa
    32941 ggtttttctg gttttgctct gaccgcgatg accacgccaa tcactaacag cgcgataccg
    33001 ctcagcgtca ttagcggcgg catttgctgg cggagtaaaa aggtgtacag caaacctgcc
    33061 agcgtttcga aaacaatcag cggcccgaga atcactgtcg gtaatcgctg gctggcgacg
    33121 ttccagcaga gtgcgccaac ccatgagcaa agcacggcta tcgcaaccat cagactaata
    33181 aacaccagcg gacgggggcc aaagggtaag gagaagtccg gcgtttgcat attcagccag
    33241 taacaggcga cgagatagcc gatgagagaa accggcagcg tgaccagcgc ctgcgccgtc
    33301 gcccacatca tcggatgttt gtcgggattt tcccgcagcc agcgggcgtt gcgcagggca
    33361 taccatgccc agcagaccac ggaaactaac gccagcacga tgcctgaggt ataacgtgcc
    33421 cagtcaaaat cggggagtcc gtggtttaac tcagcaatat tcacacacgc caggccgatg
    33481 ccaatacaaa ccagtgccgg ggcgagtttt ccccatgcga gtttgccgtc gcgctggcta
    33541 taaagcagat tggcaaaaac gggaatcacc accggcaggg tgccgataat catcgtggaa
    33601 acaggcgcgc cagtacgttg aatggcactg gcaaggcaga agtaatagat aaggttgccc
    33661 atcatggtga gcatcaaggc ggtaagccag tcgcgacgcg ccaactgacg cagacgcacg
    33721 cgtcccagcc aggcaatggg cagcgcaatt aaccctaacg ccagataacg ccccatcgac
    33781 tgcaacatcg ccgggtattc cggtacgatc aacggcccga caaaaataag cccccacatc
    33841 aaccctgcta acagggcgta cagcacgccg ctaatcatta ctggcatcca ttggtctgtc
    33901 agaagaaagt tcaggaagca gacatgattg ctcccggcat cccgtcatta tcagagagga
    33961 tgcgggagga tgccacttta attaactagt tattaacatt aaaaaaatgg cttacgcttt
    34021 cggctaacgt tgtcgttggg cggccaatca atttgctaag cgttttgctg tcatcaaaca
    34081 gaccgccttt cgatgcgcca acgtcagaat ccgccagcat atccgccagt ccatcaggca
    34141 gtccgacgct tttcagtgcc gcagcgaaat cggcttcgct caggttttga taggtaatcg
    34201 gtttgccgct ctgtttggtg agttctgccg ctaactgtgt cagcgtccag gcactatcgc
    34261 ccgccagttc gtaaaccttg ccttcatgac cggcttcgct aattacgcgc gccgcagcta
    34321 ccgcataatc agcccgcgtt gctgaggcaa ttttgccatc gcccgcggca ccgataaata
    34381 cgccgtgttc cagtgctgcc ggggcgctgg cgaggtagtt ttcggtgtac cagccgttgc
    34441 gcagcagagt gtaaacgatg ccagaatcag ccagcatttt ctccgtctcg atgtgctcat
    34501 cggcgaggcc gagcggggag gaatcggcat gtagcaggct ggtataagcg ataaatttca
    34561 cgtcggccgc ctttgcggca ttaataacat tacgatgctg cggggcacgt tgacccactt
    34621 cgctggaaga gatcagcagt agtttttcca ctccctgaag cgcagatgtc agtgcggctt
    34681 catcgccgta atcagcctga cgcacggtaa tgccttgtgc tgtcagtacc tgtgcttttg
    34741 ccggattacg aacgatagcc actatttggc tggcaggaac cgttttcatc aaggattcaa
    34801 taacatagtg accaagttgg ccagtggcac cagtaatagc gatcatggga agtctccatc
    34861 gttttgtctt gtgtttaaat acgctaacac ttaaacttac ttttagtaag tacgtacaaa
    34921 aaggtaagta tgaaatgagt caggttagcc tgtcgcaaca actcaaagag ggaaatctct
    34981 tcgctgaaca gtgtccgtcg cgcgaggttt tgaaacacgt caccagccgt tggggggtat
    35041 tgattctggt agcgctacgc gaaggtactc atcgctttag cgacctgcgg cgcaaaatcg
    35101 gcggggtgag tgaaaagatg cttgcgcagt cgttacaggc gctagagcag gatggttttc
    35161 ttaaccgtat cgcgtatccg gtggtgccgc cgcatgtgga atatagcctc acgccgctgg
    35221 gcgagcaggt gagcgaaaag gttgctgcac tggcggactg gattgagttg aatttgcccg
    35281 aggtgttagc ggtgcgggat gaacgtgcgg cataactgcc ggatgcgctg cgcttatccg
    35341 gactacggtt catggcttgt agggcggata agatgcgtca gcatcgcatc cgccaataaa
    35401 tgccggatgc ggttgatgat ttgtaggccg aaaaagaagt gttttactta ctcagatcca
    35461 cctgataaat cgcaaacccg atatcatcgg tcgcgacttt attcatcgga tactgccctt
    35521 tctctttaat aaacgctgcg gctttatctg acggagaggt ttcgaaacgg atatccagtt
    35581 tcttatcgcc agctattggt gctaaacgcc agttgttatc tgccgccggg tgaatttccc
    35641 ccgcacgttt cgactcatca gcaatccacg ctgccagcac cgagcggttc tcatccggtg
    35701 aagcaaaagc gatatggctg tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt
    35761 tattggtggc aacgaggaac atggcgttcg gatcaatcgg cttgccgtta aaggtcaggt
    35821 tcttaatcct ttccgcattg gcattaatca tctggcactc gccgtcataa cgggcgggct
    35881 gggtaacatc aatctgataa ttcacaccat caataacatc aaagttatag gtgcggaaac
    35941 catcccagtt gatgagtgac tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc
    36001 cggcggagca ctccagccac tctttcacct ctttaccgct ggctttcacc acaatcagcg
    36061 tattggggta gagataaaga tcggcggcat tacggaaggt caactggcct ttttccacct
    36121 ccacatagct tgcggggtca tttttgcgac caccaacttt aaacggtgcg gcagctgaaa
    36181 gcactggcag ttttgccaga tccggatcgc cctgaatgta atgctcgaca tacgcttttt
    36241 gcgcgttgtt caccacctgc acggtcggat cgtcctgcac aagcgccaga tagctataca
    36301 tattgtcggc agatttaccg attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg
    36361 ctttgagtgt ttctaccagc ttgctgtctt ccgccgcgag ggatttttta ttcgcgatgt
    36421 cgtaaatcgg ccgtgcttcc gctttcgcct gcgtcacctg ccatttaccg ctgtcattac
    36481 tgagttgtaa gtcgaccaca ccaagatgat cgccccacat gcctggcatt accgccggaa
    36541 caccattcag cgtgcctttc gcgatatcag ccccttcgat atcagcaaaa tctttacctg
    36601 ggaaaacggc gtgagcatgg ccaaacataa tggcgttaac gcccggaatt tcactgaggt
    36661 aataaactga gttttccgcc atcactttat acggattggc agacagcccg gaatgcgcca
    36721 gaacgacaac gacatcggca cctttctcgc gcatttcagg cacgtatttg cgcacggttt
    36781 cggtaatatc attcaccgtc actttcccgg ataaattagc tttatcccag cccatgattt
    36841 gtggcgggac gacgccaata taaccaatct tcagcgtctg tttttttccg tctttatcga
    36901 ccacttcggt gtctttaatt aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa
    36961 tgacgttggc atttacataa gggaatttcg ctcccgccag cgcatttttc agataatcca
    37021 gaccgtagtt aaactcgtgg ttgccaagcg tgccgacggt atagtccagc gtatttaatg
    37081 ccttatagac cgggtgaata tcacctgctt ttaatccttt cgccgacatg taatctgcca
    37141 gcggactccc ctgaatcaaa tcgccgttat cgaccagtac gctgtttttc acttcattgc
    37201 gggcatcgtt aatcaggctt gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt
    37261 tgtaataatc gaaatccatc atgttgctat gcagatcagt ggtttccatg atacgcagat
    37321 cgaccgtcgc tgcattcaca ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact
    37381 taatcatcag ggacatcctt ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc
    37441 ttacttatag aacagtgaag aatgccacaa ttttacgctt tgaaaatgat gacactatca
    37501 cagttggcgc attcattaac gatagggtat aagtaaaaca ataagttaac accgctcaca
    37561 gagacgaggt ggagaaatgt tagatcaagt atgccagctt gcacggaatg caggcgatgc
    37621 cattatgcag gtctacgacg ggacgaaacc gatggacgtc gtcagcaaag cggacaattc
    37681 tccggtaacg gcagcggata ttgccgctca caccgttatc atggacggtt tacgtacgct
    37741 gacaccggat attccggtcc tttctgaaga agatcctccc ggttgggaag tccgtcagca
    37801 ctggcagcgt tactggctgg tagacccgct ggatggtact aaagagttta ttaaacgtaa
    37861 tggcgaattc accgttaaca ttgcgctcat cgaccacggt aaaccgattt taggtgtggt
    37921 gtatgcgccg gtaatgaacg taatgtacag cgcggcagaa ggcaaagcct ggaaagaaga
    37981 gtgcggtgtg cgcaagcaga ttcaggtccg cgatgcgcgc ccgccgctgg tggtaatcag
    38041 ccgttcccat gcagatgcgg agctgaaaga gtatctgcaa cagcttggcg aacatcagac
    38101 cacgtccatc ggctcttcgc tgaaattctg cctggtggcg gaaggacagg cgcagctgta
    38161 cccgcgtttc gggccaacga atatttggga taccgccgct ggtcatgctg tggctgcagc
    38221 tgccggagcg cacgttcacg actggcaggg taaaccgctg gattacactc cgcgcgagtc
    38281 gttcctgaat ccggggttca gagtgtctat ttattaatta atttatgcag caggtccatc
    38341 acttgctgca cctcttcctg agtaagcgcc ccgtctttgg cccattgcac gcgaccgtct
    38401 ttatccagca ccactacagc ggaactttct tcatccagct gccaggcacc gcgtgcgacg
    38461 ccattgctat caacaataaa ctgcgactag ggataaagct ttttattact ctccagactg
    38521 ctgcgcacaa acatccctga acccggaatc gcgtcgtcgg tgttaacaat ggtggtggtc
    38581 tggtaacgat cgtgcggtaa cttcgctgat ttaatcgctt caatcagcgt cgcgtttttc
    38641 tcttttgcag aggtgcgacc agcaatatgt tgcagtactc gcacttttcc cactaactgc
    38701 gcgctgttcc aggttttgta gctaaactga tctttatcaa gcaccaactc gccacgatcg
    38761 gtgatgccaa tcggcggcac tcgctgaccg gtttcgaact gatgtgcgga agccatcatc
    38821 ggcaacagca ggcaggtgag tgccagaatc ttgcgtaggg tcatggtgtt tccttcttat
    38881 gatatgcagg tgatccgacc acttgggtct gagttttaat catatgtgct atttatcgaa
    38941 tttcccgcaa gtgtgatgcc agtttgcggt caagcgcaca aatcatatga aaaatgaatg
    39001 cttatactga agaccgcgct tcggtaaaaa gataattctg aataattgta acctttaggt
    39061 aaaaaaagtt atacgcggtg gaaacattgc ccggatagtc tatagtcact aggcattaaa
    39121 atttgcgcct cataatagtt gggccgattg tggcaccgca aaggcgtaat actcagcagg
    39181 agataacaat gaaaattttc caacggtaca acccacttca ggtggcgaag tacgtaaaga
    39241 tcctgttccg tggacggtta tacatcaagg acgttggcgc ttttgaattt gataagggta
    39301 agattcttat cccaaaagtg aaggataagc tgcatttgtc agtgatgtcc gaagttaacc
    39361 gtcaggttat gcgtctgcaa acagagatgg cttaaccaaa gtgctatgca gtaaaaagtg
    39421 ctatgcagta ataagacggc tcctgattca ggagccgttg atgtttctgg ggttatgcga
    39481 cgctttcttc tttatcttta gcgtcaggca gttttggcga aagggcggtg gctttgctgt
    39541 cgattcgggt caccagcagc tggtcgatgc ggtagttatc gatatccacc acttcaaatt
    39601 tgtagccggc gaatttcacc gaatcggtgc gtttcgggat cttacgcagc ataaacatca
    39661 taaagccgcc gatggtttcg tagttgcccg actgcgggaa ctcgtcaata tccagcacgc
    39721 gcatgacgtc gtcaataggc gtgccgccat ctatcagcca tgagttctca tcacgggcga
    39781 caatctgctc ttccagcccc tgaccgacca gatcgcccat cagcgtggtc atcacgtcgt
    39841 tgagggtgat gatccccact accagtgcgt actcattcat gatcaccgcg aagtcttcac
    39901 ctgcggtttt aaaactttcc aacgcctctg aaagggttaa cgtatccggc acaatcagcg
    39961 tattgcgaat ttgtacgccg ctgttcagtg ccaggctttg gttagccagc acgcggttca
    40021 gcaggtcttt agaatcgaca tagccgatga tgtggtcaat atcttcatta cagacgagaa
    40081 acttagagtg cggatgttcc gcaaccttat tcttcagact ttgctcatct tcgtggagat
    40141 caaaccaaat cacgttttca cgcggtgtca ttgaagacgg aacggtacgg gattccagct
    40201 caaagacgtt ttcaatcagc tcgtgttcct gtttacgtaa cacgcccgcc agcgcaccgg
    40261 cttccactac cgcgtagata tcatcagaag tgatgtcatc tttacgtacc attggcagtt
    40321 tgaaaatacg gaagattatg ttcgccagtc cgttgaagaa ccacaccagc ggggtgcaaa
    40381 cgtacaggca gaagcgcatc gggttgatga tacgcaaagc cacagcttct ggcgcaatca
    40441 taccgatgcg tttcggggtt aaatccgcaa acaggataaa catgccagtc actaacgaga
    40501 aagagagaat aaagctcagt tgctcagaga gttctgccga catatagcgg gagaacaggc
    40561 tgtgaaaagc tggagaaaat gccgcatcac cgacgatacc gccgagaatc gccactgcgt
    40621 tcagaccgat ttggaccacg gtaaagaaca tgccgggatt ttcctgcata ttcagaacgc
    40681 gttgggcatt tatattgcct tcatcagcca gcagtttaag tttgattttg cgtgaggcgg
    40741 caagcgagat ctcggacatc gagaagaacg cacttacagc gatcaaacag agtatgacta
    40801 aaatactgtt taacatatct tatccgacct ttcaggccag atcctcggaa ggggaagttg
    40861 attaatttgt gtgaaataca cattgaaagc cgattcgaag aaagtgaacc ggcattttca
    40921 gtggctagta tagcgtaagg tactgtaaag ccgccagagg gttaaaattc agacagctga
    40981 aaaatgaaaa actgcctgat gcgctatgcc gaacaggcct acgcagtccc cgcaatatat
    41041 tgaatttgga cgattttgta ggcctggcaa ggcgtttacg ccgtatccgg cataaacaaa
    41101 atgcacttta tcaacaatct acatttgtac ccgtaacgct atgcttccgg cggcagacag
    41161 acgccaattc cgccaattcc acagtaacca tacgggtttt tatgcagata ttgctggtgg
    41221 tcatcttcgg cataataaaa cggtgtggcg ttagcgattt ccgtggtgat gcgacgatcg
    41281 tcatcggcgg caagcatcgc cgcctgaaaa cgttccagac tggcgcgagc ggcggcatcc
    41341 tgttctgggg tcagcggata aatcgctgaa cgatactgcg tgccgtggtc attgccctga
    41401 cgcattccct gggcgggatc gtgattctcc caaaatactt gtagcaactg ctcatagctg
    41461 atgacggatg gatcgtaaac aatgcgcacc gcttcggcat ggccagtatc gccggagcac
    41521 acttcccgat aagtcggatt tggcgtatag ccgccggtat agcctgcggc ggtgctgtaa
    41581 acgccgggta actgccagaa cagacgctcc acaccccaga aacaacccat cgcaaaaatg
    41641 gcaatctcca ttccgtcagg tacattggtc attgagtgac cgttgaccgc atgcagtgtg
    41701 gctacgggca tcggggtgtt acgtccaggc agggcatcgg cgggggaaac cagatgcttt
    41761 ttatcaaata aactcatggt gtcgctctcc cgaaaatcgg tcattggggt taaggttgta
    41821 acaagaggcg tatttgcaca caataaccac cgtgaatagg tctaaagtaa aacataagaa
    41881 atattttggg tttagtctgc tttttaatcc atattactgg atttttgtta agccgtttaa
    41941 cggcgttcca ggggcaggaa aaaaaggata ttcaggagaa aatgtgcgct atatccgaca
    42001 gttatgctgt gtaagcttac tctgcttaag cggatctgcc gtcgccgcga acgtccgtct
    42061 acaggtcgag gggttatcgg gacagctgga aaagaacgtt cgcgcgcagc tttctacgat
    42121 tgaaagtgat gaagtgacgc cagaccgtcg ctttcgcgca cgcgtcgatg atgccatccg
    42181 cgaaggtctg aaagcgctgg gctattacca gccgaccatt gaatttgatc tccgtccacc
    42241 gccaaagaaa gggcggcagg tattgatcgc caaagtcacg ccaggcgtgc cggtgttaat
    42301 tggcggcacc gatgtggtat tgcgcggcgg cgcgcggacc gataaagact atttgaaatt
    42361 gctcgatact cgcccggcta ttggtacggt actgaaccag ggcgattatg aaaatttcaa
    42421 aaagtcctta accagcattg cgttacgtaa aggttatttc gatagcgaat ttaccaaagc
    42481 acagctgggc attgcgctcg gcctgcataa agccttctgg gatattgatt ataacagtgg
    42541 cgaacgttac cgctttgggc atgtgacctt tgaaggatca caaatccgcg atgaatacct
    42601 gcaaaatctg gtgccgttta aagagggcga tgagtacgaa tcgaaagatc tggcagaact
    42661 gaaccgtcga ctttctgcta ccggctggtt taactcggtg gtggtggctc cacaatttga
    42721 taaagcgcgc gaaacgaaag tattaccatt gacgggcgtg gtttcgccgc gaacagaaaa
    42781 caccatcgaa accggcgtcg gttactctac ggacgtggga ccgcgcgtga aagcgacgtg
    42841 gaaaaagccg tggatgaact cttacggtca cagtttgacc accagtacca gtatttccgc
    42901 gccggaacag atcctcgact tcagctataa aatgccgctg ctgaagaatc cgctggaaca
    42961 atattatttg gtgcagggcg gttttaagcg cactgacctg aacgataccg agtctgactc
    43021 cactacgctg gtggcttctc gctactggga tctctccagc ggctggcagc gtgccattaa
    43081 cctgcgctgg agtttcgacc actttaccca gggtgaaatt accaacacca cgatgctgtt
    43141 ttatcctggg gtgatgatta gccgcacgcg ttctcgtggt ggcctgatgc caacctgggg
    43201 cgactcgcaa cgctactcta tcgactactc caacacggcc tggggctcgg atgttgattt
    43261 ctccgttttc caggcgcaga acgtctggat ccgcacactg tacgatcgcc atcgttttgt
    43321 gacacgcggc acgttgggct ggattgaaac gggtgatttc gacaaagtac cgccggatct
    43381 gcgtttcttc gccggtggcg atcgcagtat tcgcggctac aaatacaaat ctatcgcccc
    43441 gaaatacgcc aacggtgacc tgaaaggggc ctcgaagctg ataaccggat cgctggaata
    43501 ccagtacaac gtgaccggaa aatggtgggg cgcggtgttt gtcgatagcg gcgaagcggt
    43561 aagcgatatt cgccgcagcg actttaaaac cggtaccggt gtcggcgtgc gctgggaatc
    43621 gccggtcggg ccaatcaaac tcgattttgc cgtaccggtc gcggataaag acgaacacgg
    43681 gttacagttt tacatcggtc tggggccaga attatgagtt tatggaaaaa aatcagcctc
    43741 ggcgtggtta tcgttatctt actgttgctg ggatcggtgg cgtttctggt gggcaccacc
    43801 agcggcctgc atctggtatt taaagcggcg gatcgctggg tgccaggact ggatattggc
    43861 aaggtcaccg gcggctggcg tgatctcacc ttgtctgacg ttcgttatga gcagccaggc
    43921 gtggcggtaa aagcgggcaa tctgcatctg gcggtcgggc ttgagtgcct gtggaacagt
    43981 agcgtttgta ttaatgatct ggcgctgaaa gacattcagg tcaacatcga cagtaaaaaa
    44041 atgcctcctt ctgaacaggt tgaagaagag gaagatagcg gtccgctgga tctctccacg
    44101 ccgtatccca ttaccctgac acgggtggcg ctggacaacg tcaacatcaa gattgatgac
    44161 accacggtat cggtgatgga cttcacctcc ggcctcaact ggcaggagaa aaccctgacc
    44221 ctgaaaccga catcgctgaa aggcctgttg attgctttgc cgaaagtggc ggaagcggcg
    44281 caggaagaag tggtcgaacc gaaaattgaa aatccacagc cggaggaaaa gccgctcggc
    44341 gaaacgctga aagatctctt ttctcgcccg gtattgccgg aaatgaccga cgtgcatttg
    44401 ccgcttaacc tgaacattga agagtttaag ggcgagcaac tgcgcgtgac gggcgacacg
    44461 gacatcaccg tgagcaccat gctgctgaaa gtgagcagca ttgacggcaa tactaaactg
    44521 gacgccctgg atatcgactc cagtcaaggg atcgtcaacg ccagcggcac ggcgcagctg
    44581 tcagacaact ggccggtgga tatcaccctc aacagcacac tgaacgtgga gccgttgaaa
    44641 ggtgaaaagg tgaagctgaa agtgggcggt gcgctgcgcg aacagctgga gataggcgtt
    44701 aacctttccg gtccggtgga tatggattta cgcgcccaga cgcgactggc ggaagccgga
    44761 ttgccgctca acgtggaagt gaacagcaaa cagctttact ggccgttcac tggtgagaag
    44821 cagtatcagg cggatgatct gaaactgaaa cttaccggca aaatgaccga ttacacgctc
    44881 tctatgcgca cggctgttaa gggccaggag atcccgccag ccaccattac ccttgatgcc
    44941 aaaggtaatg aacagcaggt caatctcgac aaacttaccg tcgcggcgct ggaagggaaa
    45001 acggaactta aggcgttgct cgactggcag caggccatta gttggcgcgg tgagctaacg
    45061 cttaacggca ttaacaccgc caaagagatc ccggagtggc cgtcgaaact caatggcttg
    45121 attaaaaccc gcggtagcct gtacggcggc acctggcaga tggaggtgcc agaactgaag
    45181 ctgaccggta acgtcaaaca gaacaaagtg aacgttgacg gcacgctgaa aggcaacagt
    45241 tatatgcagt ggatgatccc agggctgcat ctggaactgg ggccaaacag tgccgaagtg
    45301 aaaggcgagc tgggggtaaa agatctcaat cttgatgcca ccatcaacgc gccggggctg
    45361 gataacgcgc tgccggggct tggcggtaca gcgaaagggc tggtgaaagt acgcggcacg
    45421 gtggaagcgc cacaactact ggcagatatc accgcgcgcg gcctgcgctg gcaggaactt
    45481 tccgtggcgc aggttcgcgt ggaaggcgac attaaatcca ccgatcagat cgccgggaaa
    45541 ctcgacgtac gcgttgagca aatttcgcaa ccggatgtaa atatcaacct cgtcaccctg
    45601 aatgccaaag gcagtgaaaa gcagcacgag ctacagttgc ggattcaggg cgagcctgtc
    45661 tccgggcagc ttaatctggc aggaagtttt gatcgcaaag aagaacgctg gaagggaact
    45721 cttagcaata cccgcttcca gacgccggtc ggcccgtggt cgctgacccg cgatattgcg
    45781 ctggattacc gcaataagga gcaaaaaatc agcatcgggc cacactgctg gcttaacccg
    45841 aatgcggaac tgtgcgtgcc gcaaactatc gatgcgggtg ccgaagggcg tgcggtggtg
    45901 aatctcaacc gcttcgacct ggccatgctg aaaccattta tgccagaaac cactcaggcc
    45961 agcggtatct tcacgggtaa agcggacgtt gcctgggaca ccacgaaaga ggggctgccg
    46021 cagggcagta tcaccctttc ggggcgtaat gtgcaggtga cgcaaaccgt caacgatgcg
    46081 gcgctgcccg tggcgtttca gacgctgaat ctgacggcgg agttgcgtaa caaccgtgcc
    46141 gaattgggct ggaccatccg cctgaccaat aacggccagt ttgatggtca ggtgcaggtg
    46201 accgatccgc aaggccgccg taatcttggt ggcaacgtca atatccgtaa cttcaacctt
    46261 gcgatgataa accccatctt tacccgtggg gaaaaagccg cggggatggt gagtgccaac
    46321 ttgcgtctgg gtggtgatgt gcaaagcccg cagttgtttg gtcagcttca ggttacgggg
    46381 gtggatatcg acggcaactt tatgccgttt gatatgcaac cgagccagct tgcggtcaac
    46441 tttaacggta tgcgctcgac gcttgcaggt accgtgcgga cccagcaggg tgaaatctac
    46501 ctgaacggtg atgccgactg gagccagatt gaaaactggc gggcgcgggt aacagcgaag
    46561 ggcagtaaag tgcgtatcac cgtgccgccg atggtacgaa tggatgtatc gccagatgtt
    46621 gtattcgagg ctacaccaaa cctgtttacc ctcgatggtc gcgtggatgt tccgtgggcg
    46681 cgcatcgtgg tgcacgatct gccggaaagc gcagtaggcg tttccagcga tgtggtgatg
    46741 cttaacgata acctgcaacc ggaagagccg aaaacggcgt cgattccgat taacagtaac
    46801 ctgactgtcc acgttggcaa caatgtgcgc attgacgcct ttggcctgaa agcgcggctg
    46861 acgggcgatc tcaacgtcgt tcaggacaaa caagggctgg gcctgaacgg gcagatcaac
    46921 atccctgaag ggcgcttcca tgcctatggt caggatctga ttgtgcgtaa gggtgagcta
    46981 ctgttctctg gtccgccaga tcaaccgtat cttaatatcg aagctattcg taacccggat
    47041 gctacagaag acgacgtcat cgccggagtt cgcgtcactg gtctggcgga cgaaccgaaa
    47101 gcggagatct tctctgaccc ggcgatgtcg caacaagctg cattgtctta tttgctacgt
    47161 ggacaagggc tggagagcga tcagagcgac agcgcggcga tgacctcgat gctgattggc
    47221 ctgggggttg cgcaaagtgg ccagattgtg ggtaaaatcg gcgagacgtt tggcgtaagc
    47281 aatttagcgc tcgacaccga gggagttggc gactcctccc aggtggtggt cagcggctat
    47341 gtattgccag gcctgcaagt gaaatacggc gtgggtatat ttgactctat agcaacactc
    47401 acgttacgtt atcgcctgat gcctaagcta tatctggaag ccgtgtctgg tgtagaccag
    47461 gcactggatt tgctctatca gttcgagttt tagcaatgcg aatatttgtc tacggcagtt
    47521 tacgccacaa acaaggcaac agtcactgga tgaccaatgc ccagttactg ggcgatttca
    47581 gtattgataa ctaccagttg tatagcctgg gccactatcc aggcgcagtt ccggggaacg
    47641 gaacagtaca cggtgaagtt tatcgtattg acaacgccac gctggccgaa cttgatgcct
    47701 tgcgcaccag gggcggtgaa tacgcgcgcc agttgattca gacgccgtac gggagtgcat
    47761 ggatgtacgt ttatcaacga cccgtcgatg gattaaagct aattgaaagc ggcgactggt
    47821 tagacaggga taagtaataa taaaagcacg ccaccttcgg gtggcgcgaa aattgatgta
    47881 atgcaatgaa aaataaagga ttatttttct cgttgtccac attttgccat agcgatacat
    47941 cttagcccgt aatgattcgc gggctaagca tatacaaaat gttaagcctg atacgggggt
    48001 cctatgtttg atagggaaat gataaacgaa aataaagagg cgttttttct gtatgttaac
    48061 ggtagagggg agttgtcggc tcaatgcgtg ataaaggtgg ctgaaaaagg ggattattta
    48121 caaggattct ctttaactcg tcgcgcattt cgtacctttt tgaaagaccg tgttattcag
    48181 atgttttcta gcgaccagga gttgaataag gttgcgtctg aaac
//
LOCUS       DAZKHK010000017        47874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKHK010000017 DAZKHK010000000
VERSION     DAZKHK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.017"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..703)
                     /gene="pdeC"
                     /locus_tag="ACHOS5_001050"
     CDS             complement(<1..703)
                     /gene="pdeC"
                     /locus_tag="ACHOS5_001050"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9775905.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQR"
     gene            1132..1413
                     /gene="yjcB"
                     /locus_tag="ACHOS5_001051"
     CDS             1132..1413
                     /gene="yjcB"
                     /locus_tag="ACHOS5_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP9775906.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(1512..2048)
                     /gene="ssb1"
                     /locus_tag="ACHOS5_001052"
     CDS             complement(1512..2048)
                     /gene="ssb1"
                     /locus_tag="ACHOS5_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP9775907.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2303..5125
                     /gene="uvrA"
                     /locus_tag="ACHOS5_001053"
     CDS             2303..5125
                     /gene="uvrA"
                     /locus_tag="ACHOS5_001053"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9775908.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5160..5516)
                     /gene="yjbR"
                     /locus_tag="ACHOS5_001054"
     CDS             complement(5160..5516)
                     /gene="yjbR"
                     /locus_tag="ACHOS5_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9775909.1"
                     /translation="MTISELLQYCMAKPGAGQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5520..5936)
                     /gene="yjbQ"
                     /locus_tag="ACHOS5_001055"
     CDS             complement(5520..5936)
                     /gene="yjbQ"
                     /locus_tag="ACHOS5_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP9775910.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6047..6760)
                     /gene="aphA"
                     /locus_tag="ACHOS5_001056"
     CDS             complement(6047..6760)
                     /gene="aphA"
                     /locus_tag="ACHOS5_001056"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP9775911.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            7037..8857
                     /locus_tag="ACHOS5_001057"
     CDS             7037..8857
                     /locus_tag="ACHOS5_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9775912.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE"
     gene            8850..10208
                     /locus_tag="ACHOS5_001058"
     CDS             8850..10208
                     /locus_tag="ACHOS5_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFP9775913.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(10197..11270)
                     /locus_tag="ACHOS5_001059"
     CDS             complement(10197..11270)
                     /locus_tag="ACHOS5_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFP9775914.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(11282..12781)
                     /locus_tag="ACHOS5_001060"
     CDS             complement(11282..12781)
                     /locus_tag="ACHOS5_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFP9775915.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     NWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            complement(12993..13865)
                     /gene="sucD"
                     /locus_tag="ACHOS5_001061"
     CDS             complement(12993..13865)
                     /gene="sucD"
                     /locus_tag="ACHOS5_001061"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9775916.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHEMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWNVIK"
     gene            complement(13878..15047)
                     /gene="sucC"
                     /locus_tag="ACHOS5_001062"
     CDS             complement(13878..15047)
                     /gene="sucC"
                     /locus_tag="ACHOS5_001062"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9775917.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVDSLDDAAKRIIALLN"
     gene            complement(15069..16487)
                     /gene="lpdA"
                     /locus_tag="ACHOS5_001063"
     CDS             complement(15069..16487)
                     /gene="lpdA"
                     /locus_tag="ACHOS5_001063"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9775918.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            complement(16499..17653)
                     /gene="odhB"
                     /locus_tag="ACHOS5_001064"
     CDS             complement(16499..17653)
                     /gene="odhB"
                     /locus_tag="ACHOS5_001064"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9775919.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL"
     gene            complement(17686..20429)
                     /locus_tag="ACHOS5_001065"
                     /pseudo
     CDS             complement(17686..20429)
                     /locus_tag="ACHOS5_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            complement(20611..21804)
                     /gene="tyrB"
                     /locus_tag="ACHOS5_001066"
     CDS             complement(20611..21804)
                     /gene="tyrB"
                     /locus_tag="ACHOS5_001066"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP9775920.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDSYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAKVVAAVLNDEALKAGWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLRIAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            complement(22057..23136)
                     /gene="alr"
                     /locus_tag="ACHOS5_001067"
     CDS             complement(22057..23136)
                     /gene="alr"
                     /locus_tag="ACHOS5_001067"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9775921.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(23189..24604)
                     /gene="dnaB"
                     /locus_tag="ACHOS5_001068"
     CDS             complement(23189..24604)
                     /gene="dnaB"
                     /locus_tag="ACHOS5_001068"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9775922.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24687..25670
                     /gene="qorA"
                     /locus_tag="ACHOS5_001069"
     CDS             24687..25670
                     /gene="qorA"
                     /locus_tag="ACHOS5_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP9775923.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(25835..26077)
                     /gene="pspG"
                     /locus_tag="ACHOS5_001070"
     CDS             complement(25835..26077)
                     /gene="pspG"
                     /locus_tag="ACHOS5_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP9775924.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(26211..27206)
                     /gene="dusA"
                     /locus_tag="ACHOS5_001071"
     CDS             complement(26211..27206)
                     /gene="dusA"
                     /locus_tag="ACHOS5_001071"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP9775925.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            27362..27877
                     /gene="zur"
                     /locus_tag="ACHOS5_001072"
     CDS             27362..27877
                     /gene="zur"
                     /locus_tag="ACHOS5_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP9775926.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(27919..28128)
                     /gene="yjbJ"
                     /locus_tag="ACHOS5_001073"
     CDS             complement(27919..28128)
                     /gene="yjbJ"
                     /locus_tag="ACHOS5_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9775927.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(28244..29569)
                     /gene="dinF"
                     /locus_tag="ACHOS5_001074"
     CDS             complement(28244..29569)
                     /gene="dinF"
                     /locus_tag="ACHOS5_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP9775928.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            complement(29642..30250)
                     /gene="lexA"
                     /locus_tag="ACHOS5_001075"
     CDS             complement(29642..30250)
                     /gene="lexA"
                     /locus_tag="ACHOS5_001075"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9775929.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(30360..30728)
                     /gene="dgkA"
                     /locus_tag="ACHOS5_001076"
     CDS             complement(30360..30728)
                     /gene="dgkA"
                     /locus_tag="ACHOS5_001076"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9775930.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            30899..33322
                     /gene="plsB"
                     /locus_tag="ACHOS5_001077"
     CDS             30899..33322
                     /gene="plsB"
                     /locus_tag="ACHOS5_001077"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP9775931.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(33477..34349)
                     /gene="ubiA"
                     /locus_tag="ACHOS5_001078"
     CDS             complement(33477..34349)
                     /gene="ubiA"
                     /locus_tag="ACHOS5_001078"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP9775932.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(34362..34859)
                     /gene="ubiC"
                     /locus_tag="ACHOS5_001079"
     CDS             complement(34362..34859)
                     /gene="ubiC"
                     /locus_tag="ACHOS5_001079"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP9775933.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(35038..35958)
                     /gene="malM"
                     /locus_tag="ACHOS5_001080"
     CDS             complement(35038..35958)
                     /gene="malM"
                     /locus_tag="ACHOS5_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP9775934.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(36190..37530)
                     /gene="lamB"
                     /locus_tag="ACHOS5_001081"
     CDS             complement(36190..37530)
                     /gene="lamB"
                     /locus_tag="ACHOS5_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP9775935.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(37602..38717)
                     /gene="malK"
                     /locus_tag="ACHOS5_001082"
     CDS             complement(37602..38717)
                     /gene="malK"
                     /locus_tag="ACHOS5_001082"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP9775936.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            39082..40272
                     /gene="malE"
                     /locus_tag="ACHOS5_001083"
     CDS             39082..40272
                     /gene="malE"
                     /locus_tag="ACHOS5_001083"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP9775937.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            40426..41970
                     /gene="malF"
                     /locus_tag="ACHOS5_001084"
     CDS             40426..41970
                     /gene="malF"
                     /locus_tag="ACHOS5_001084"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP9775938.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            41985..42875
                     /gene="malG"
                     /locus_tag="ACHOS5_001085"
     CDS             41985..42875
                     /gene="malG"
                     /locus_tag="ACHOS5_001085"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP9775939.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(42969..43379)
                     /gene="psiE"
                     /locus_tag="ACHOS5_001086"
     CDS             complement(42969..43379)
                     /gene="psiE"
                     /locus_tag="ACHOS5_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP9775940.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            43593..43871
                     /locus_tag="ACHOS5_001087"
     CDS             43593..43871
                     /locus_tag="ACHOS5_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9775941.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(43918..46014)
                     /gene="yjbH"
                     /locus_tag="ACHOS5_001088"
     CDS             complement(43918..46014)
                     /gene="yjbH"
                     /locus_tag="ACHOS5_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9775942.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPKYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLGGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(46014..46751)
                     /gene="yjbG"
                     /locus_tag="ACHOS5_001089"
     CDS             complement(46014..46751)
                     /gene="yjbG"
                     /locus_tag="ACHOS5_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9775943.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGTPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(46748..47386)
                     /gene="yjbF"
                     /locus_tag="ACHOS5_001090"
     CDS             complement(46748..47386)
                     /gene="yjbF"
                     /locus_tag="ACHOS5_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP9775944.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLATDPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(47501..47743)
                     /gene="yjbE"
                     /locus_tag="ACHOS5_001091"
     CDS             complement(47501..47743)
                     /gene="yjbE"
                     /locus_tag="ACHOS5_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP9775945.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
BASE COUNT        11858 a        12761 c        11866 g        11389 t
ORIGIN      
        1 gacgttgata tgaagtcgca acaatggctg caattgggcg ttttgtcgaa taaactgtcg
       61 catataaata gccatttcgt tgtacggtta aatcattcaa atgaatcagc ggagaaaacg
      121 ttgctgccga ggcctcgttg cttaacgaga aaaaggtttt cgtcaccgta tcatacacac
      181 cccattgcaa tgtcggatca tcagacatca cttcactcca gaagagaggg ttgataaccg
      241 ccacataatt tccccgctgc atgtaggtca ttttatagcc agagaaaaaa ggcgtatcac
      301 ggtaataata gatagaaacg ttaggttcac gcttataatc ggccggtgca atcgtatagc
      361 catttacaga cgctatcagc gatgagcata aaaaatggtt atcacgagca taaatcaatt
      421 cattaatata aagatagcca cgaataatat tcaacattcg cttttgatgg gctggagtgc
      481 aaacttgccc ctgatagcgt tcagctgcat cgctcactaa atctgcctga cgaatgacca
      541 gctcagattt atccaacgcc agctgagcaa aggttcggag ctgattattc acttctgatt
      601 ttgcccaaaa gaaggcaatc catagcgaca gaatgatggg aaagagaact aaaaagataa
      661 tgcccggaaa cgccagtaat tggtgtcgtg cacgatgact cattgctatc aattccctgc
      721 ctgtttatac ccggttattg caaggtgaaa ccctggctac cgacgtcttt agataaatct
      781 tacggtataa atagcacttt tgcctgatat aaaattgaga cctttttaat cgtcaccagg
      841 atgacgataa ttcattgatc ttaaaaatta atcaccttca ggaatgcaga ggcggcggga
      901 agatataccc ttaatgccgc tgaaaaaaca cataatctgt acggcaataa aagtcacggc
      961 ctaatctggc taacaaacaa ccaattgtga tatagttcac aaaattcatg aaacaaacag
     1021 agtgtttcat ttttgtgttc tgcaagcccc gcgatagcca gagtgtatca cgcctcccgt
     1081 gaacaacgcc gcgctgtcca gggcatcagc tcttttccga ggaattgttt gatggctacc
     1141 ctcaccactg gcgtggttct tcttcgctgg caacttctta gtgccgtaat gatgtttctg
     1201 gctagcacgc tcaacatccg ttttcgtcgg gctgattatg tcgggcttgc agtgatcagc
     1261 agcggtctgg gcgtagtttc cgcctgctgg ttcgcaatgg ggttgcttgg catcacaatg
     1321 gcggatatca ccgccatctg gcacaacatc gagtcggtga tgatagaaga gatgaatcag
     1381 acaccgccac aatggccaat gattttgact tgatatgtag aagcctccat aacggaggct
     1441 tcttttttat ggctggcaga tgctttaatc atccacctta aaacaatata acctattgtt
     1501 ttaaagacaa atcagaacgg aatatcgtca tcaaagtcca tcggcggctc gttagacggc
     1561 gctgccggag cggactgctg cgggcgagac tgcgcgccgc cgctgaactg attgccaccc
     1621 tgcggctgct gaggctgacc ccaaccgccc tgcggctgac caccaccgat attgccacct
     1681 gcaggagcgc caccaccctg acgaccaccc aacatctgca tggtgccgcc aacgttcacc
     1741 acgacttctg tggtatagcg atcctgaccg gattgatcgg tccatttgcg ggtacgcagc
     1801 tggccttcga tataaacctg agaaccttta cgcagatatt cgctggccac ttctgccagt
     1861 ttgccgaaca gcacaacgcg gtgccattca gtctgctctt tcatctcgcc ggtcgcttta
     1921 tcacgccagg attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg
     1981 tagcgtactt ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg
     2041 ctggccatgt tcgtgtctcc tgaaaaaaaa tcgttctgaa taagtgtaaa cgcgcgattg
     2101 taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa caaacctggc
     2161 cagacattgt tacacaacac tccgggtaat tcattccaat actgtatatt cattcaggtc
     2221 aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc gggcaaaaaa
     2281 gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc gcacccataa
     2341 tctcaaaaac atcaacctcg ttatcccccg cgacaagctt attgtcgtga ccgggctttc
     2401 gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc agcgccgtta
     2461 cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa agccggacgt
     2521 cgaccatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga cgtctcataa
     2581 cccgcgctct acggtgggga caatcaccga aatccacgac tatttgcgtc tgctgtacgc
     2641 tcgcgttggt gagccgcgct gcccggacca cgatgtaccg ctggcggcgc aaaccgtcag
     2701 tcagatggtg gataacgtgt tgtcccagcc ggaaggcaag cgtctgatgc tgctcgcgcc
     2761 aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg caagccaggg
     2821 ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc cgaaactgga
     2881 actgcaaaag aaacatacca ttgaagtggt ggttgatcgc ttcaaagtgc gtgacgatct
     2941 tacccaacgt cttgccgagt cgtttgaaac cgcgctggag ctttccggtg gtaccgcggt
     3001 agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca actttgcctg
     3061 cccgatttgc ggttacagta tgcgcgagct ggaacctcgc ctgttttcat ttaacaaccc
     3121 ggcgggagcc tgcccgacct gtgacggtct tggcgtacag caatatttcg atcctgaccg
     3181 cgtgatccag aatccggaat tgtcgctggc tggcggtgcg atccgtggct gggatcgccg
     3241 caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt tcgacgtcga
     3301 agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg gttctggcaa
     3361 agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc gtcgtcatcc
     3421 gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat ccagcgcggt
     3481 acgtgaagaa ttagccaagt ttatcagcaa tcgtccgtgc gccagctgcg aaggaacccg
     3541 tctgcgtcgg gaagcacgcc acgtgtatgt cgagaatacg ccgctgcccg ccatctccga
     3601 catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag gtcagcgggc
     3661 gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc tcgttaacgt
     3721 cggcctgaat tacctgacac tttcccgctc agcagagaca ctttccggcg gtgaagccca
     3781 gcgtattcgt ctggcgagcc agattggtgc aggcctggtt ggcgtgatgt acgtgctgga
     3841 tgagccgtct atcggcctgc accagcgcga taacgagcgc ctgctgggta cgcttatcca
     3901 tctgcgcgat ctcggtaata ccgtgattgt ggtggagcac gacgaagacg cgattcgcgc
     3961 cgctgatcat gtgatcgata tcggtccggg tgcgggtgta cacggcggtg aagtcgtcgc
     4021 ggaaggtccg ctggaagcaa taatggcggt gccagagtcg ttgaccgggc agtacatgag
     4081 cggtaaacgc aagattgaag tgccgaagaa acgcgtaccc gccaatccag aaaaagtgct
     4141 gaaactgacg ggcgcacgcg gcaacaacct gaaagacgtg acgctgacgc tgccggtagg
     4201 tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga ttaacgacac
     4261 actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac cggcaccgta
     4321 tcgcgatatt cagggactgg agcatttcga taaagtgatc gatatcgacc aaagcccaat
     4381 tggtcgtact ccgcgttcta acccggcgac ctataccggc gtgtttacac ctgtgcgcga
     4441 actgtttgcg ggcgtaccgg aatcccgtgc gcgcggctat acgccaggac gtttcagctt
     4501 taacgtccgt ggcggacgct gcgaggcctg tcagggcgac ggcgtgatca aagtggagat
     4561 gcacttcctg ccggacattt acgtaccgtg cgaccagtgc aaaggcaaac gctataaccg
     4621 tgaaacgctg gagattaagt acaaaggcaa aaccatccac gaagtgctgg atatgaccat
     4681 cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc tgcaaacgct
     4741 gatggatgtt ggcctgacgt acattcgcct cgggcagtcc gcaaccacac tttctggtgg
     4801 tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg ggcagacgct
     4861 gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc aactgctcga
     4921 cgtactgcat aaactgcgcg atcagggcaa taccattgtg gtaattgagc acaatctcga
     4981 cgtgatcaaa accgctgact ggattgtcga cctgggaccg gaaggcggca gtgggggcgg
     5041 cgaaatcctc gtctccggta cgccagaaac cgtcgcagag tgcgaagctt cgcatacggc
     5101 gcgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tcagagcggc cttcttcttt
     5161 cagagttgca ccagcaattt acgtttttct tccggcagca aattcaccgc ctgctgataa
     5221 gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag atacacggtg
     5281 ctccagtgcg ctttattcag atggcggctt ggacgcacat cgctgtgctg ctgacgtagc
     5341 agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc tacttctttc
     5401 accatcgcaa acagcacatc ttccactttg atctgcgtcg ctttccagtc gttatgcacg
     5461 ctctgtcctg cgcctggttt tgccatgcaa tattgtagca actccgaaat ggtcattttt
     5521 cactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg atgttccccc
     5581 agccagatgc cttgccaggt gccggtctga atacgccctt tatgcaccgg caatacaagc
     5641 gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc ctcatagtca
     5701 tgctcataat ttccgttatc gggaacggcg cggaggaaaa aacgctccat gtcgtggcgt
     5761 acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg cagcaacaga
     5821 tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat ttcatccgtt
     5881 accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata ccacatgtgc
     5941 tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg ataaaggtgt
     6001 gattaaaaaa acagcattcg ggagagtatt acgctctccc gctctgtcag tattctgaat
     6061 tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag gtagagttgg
     6121 aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta atatcgttat
     6181 cggaatcacc ataaaaaatt cggatatttt tatcctgcag ccattgcgat tttgtatttt
     6241 gccctggttt atcgcccgca aagatcaccg gattcatgtt ggtggcagga atatgaaaat
     6301 tatccgccag cgtttttgaa accgtttctg ttttggtcgg gctacgacca gtcacaaaga
     6361 agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc tcttttggaa
     6421 tgctgaattc atcccagcca ttgttcattt tttcccagaa cacaggattt ttcagataat
     6481 cttcgctttc tggcgagaag gtttttttgc cgcgccagaa acccggactg gaaaaaagta
     6541 ccgtatcatc gatatcaaac cccaccgcca ttggcggacg tcctgcgagg ctattttcaa
     6601 tttgtgcaac cgaaacccaa tgaattggcg cctgttcagc gagtttggct acgttagtac
     6661 ccggattaag cggtgaagga gatgaggcca aggcaacagc ggaactgttt agagcgaaca
     6721 ataagcaaac ggcactcaat gcctgtgtga tcttgcgcat atttttccct aaatagtcag
     6781 taagttgaaa cttattattg tgcgtttgtt gaaaaaacca tctgaccata acgccaaccg
     6841 cgggggaaag gaaggattat ctgcggattt gtcacataaa aagagcatga tcacatttgg
     6901 aatacctgtg cgataaacca cacattgcca cggttattct gcttacacat tcacacaatg
     6961 gcactctttt ggttctcgca aacattgttc agggaatata ctgatgataa atataaatgc
     7021 attcaatgga tgatgaatga aaatatcgtg gaattatata tttaagaaca aatggcgatt
     7081 tcacattaca agcatttcac tttttcttat catgctcgcg gtttcaatcg cttttttgca
     7141 cttgcgtttt aataccttgt ccggtaccga taaaatgcgg cttgaaatgt ataagtccac
     7201 attatattcc accatcgagc aattttatgt tttaccctat atgctctcaa cagaccatat
     7261 catccgtcag gcggtaatta cgcctgacga tatgacgtcc agcgaactca atcaacgaat
     7321 tgcacatttc aatactcaac tcaaaaccgc agcaatattt attctggata cccaaggtaa
     7381 ggccatcgct tctagcaact ggcaggaccc cggcagctat gtagggcaaa attatagcta
     7441 tcgcccctat tataaacacg ccatgtctgg cttaaatgga cgcttttacg gtattggtag
     7501 cactacaaat acaccgggat tcttcctctc tacaagtata aaagataaag gaaaaattgt
     7561 cggtgttgta gtagtaaaaa taagtcttaa tgaaattgaa aaagcatggg ccgaaggtcc
     7621 tgaaaatatt atcgtgaatg atgagcatgg gattatattt ttaagttcaa aatcgccatg
     7681 gcgaatgcga acactgcaac cgttacctgt tcaggcaaaa caaaaactac aatctacccg
     7741 ccaatatagt ctcgacaatc ttttaccggc ggattattat ccttgttata ccgtgagcaa
     7801 ttttactttc ctgaaagata aaaaagaaca actctgttta ttcccgcaat attatacgca
     7861 acaaatagcc attccagaat ttaactggaa aatgacaatt atggtcccct tagataacct
     7921 gtactggtca tgggctattt cgttagtcat tacactaatt atttacctgc tgtttttgtt
     7981 atttattaaa tactggagaa tgcgatctca tgcacaacaa ttattaacac tggcgaatga
     8041 aacattagaa aaacaggtta aagagcgtac atctgccctg gaattgatca atcaaaaatt
     8101 aatacaggag ataaaagagc gcagtcaggc tgaacaagta ttacaaatta cgcgtagtga
     8161 actggcagag tccagcaaac tggcggcgct tggacagatg gcaaccgaaa ttgcccatga
     8221 acaaaatcaa ccgttagccg ccattcacgc acttactgat aacgcgcgta ctatgctaaa
     8281 aaaagagatg tatccgcagg ttgaacagaa tctgaaacac attatttcag tgattgagcg
     8341 gatgacgcag ctcatttccg aacttaaagc atttgcctcg cgccatcgcg tacctaaagg
     8401 ttctgccgat gtcatcaaag tgatgtatag cgccgtggcg ttacttaatc acagcatgga
     8461 gaaaaataac attgagcgac gaataaaagc cccatccatg ccgttatttg tcaattgcga
     8521 tgagctcggt cttgaacaga tattcagtaa tttaattagc aacgccttag attctatgga
     8581 aggtagctct tacaaacgac tggatatcgc cattcgccag gcaaataaca aagttattat
     8641 taccattaaa gacagcggtg gcggttttgc acctgaagtt gtcgatcgca tatttgaacc
     8701 attttttacc actaaacgta gaggaatggg gttgggactg gcaatagtca gcgaaattgt
     8761 ccgaaattcg aacggcgcac tccacgccag taatcatcct gaaggcggcg cagtaatgac
     8821 attaacctgg cctgaatggg gagaagaaca tgaataataa atccagtgaa attactatag
     8881 tttatattga agatagtgac gatgtacgtt ttgcctgtga acaaacgctc acgctggctg
     8941 gttatcgtgt tatttcctgt tgcgatgcag aacacagtat tccccttatt caaagtcagg
     9001 ccaacattat tatactgacc gatgtcaggt tacctggaat atcaggacta gagctcctca
     9061 gttacatcaa cgaaatggat agcaaaattc cggttatttt aattaccgga cacggtgacg
     9121 tggaaatggc ggtggatgcc atgcgtaatg gtgcattcga tttcattgag aaaccaagta
     9181 gctcagataa attattgagt attattgcca gagcggtaga aaaacggcga ttggttttag
     9241 aaaatcagca attactggcg aatctgcaac aggaaaatgg cccggtgctg attggtcgta
     9301 gtccgcagat gcaacaactg cgtaaaatga tccttaatgt cgcagatacc ggtgccgatg
     9361 tccttattta tggtgaaaca ggttgtggta aagaagtcgt cgccagaatg ctgcatcact
     9421 ggagtacccg ccgtcagggg cagtttgttg ccttaaactg tgcaggctta ccagaaacgt
     9481 tgtttgaaag cgaaatattt ggtcatgagg cgggcgcatt taccggtgcg gttaaaaagc
     9541 gtattggaaa aattgaacat gcaaatggtg gtacgttatt tctcgatgaa atagagggaa
     9601 tgccatcagg aatgcaggta aaactgctac gtgtattgca ggaaagaacc attgaacgat
     9661 taggtgccaa tcaattaatt ccggtgaatt gccgtgttat tgcagcgacc aaggaagatt
     9721 tactgcgtcg cagcgaagaa catctgtttc gtctcgatct ctattatcgt ctgaatgtgg
     9781 ttagcctgaa tattccacct ctacgccagc gacgagaaga tatccctgaa ctgttttact
     9841 ggttcgccag tcaggcggca caaaaatata atcgtcctct gcctgatatt tctcccatgt
     9901 tactggcctg gttgcaatca caaagttggc ctggcaatgt acgtgaactt aaacacaatg
     9961 cagagcgttt tgttctcggt ttactgaccc accaccagcc agtgccaatg actcagcaag
    10021 aagagagtgg ccttaccgcc tgcattgatg ctttcgagaa aaagttaatc gaagatatgc
    10081 tgcgacaaac ggaaggccag gtcagtttga cagcgcggct attacagctg ccgcgcaaga
    10141 ctttatatga caaactcaac aaacatcaga ttcaaccgca agtttatcga ccagaatcat
    10201 ccagctaatg ctgattcctg aggatgtaac cccaaatcga tacctttgcg ggcgtaatgt
    10261 tcaactaccc aggaccagaa tgataccggg atcgtaatca tcttcgcatt gcgttcccgg
    10321 ttggcagctt ctggcatacc ggggtaaatc accggattgc cgccaggttg aggctgcgta
    10381 gctaacagat aatccgcaaa gaactgccct ttctcccctg ccagatcggt gaattgatca
    10441 ggatcgataa tcaacgcaaa aagatgatta tgcgcagatc catccgtctg atgatcttcg
    10501 gcgatcgtgt caccaccaga taagatcccc gccatcattt caatcatcag tgccaggcca
    10561 aatcctttat gctcaccaaa tggcagtaac gcccctggag gattgctgaa taacacccca
    10621 ggatcattgc ttggattccc ctgtgcatcg atcaattgtc ccggcgcggc ctgcttcccg
    10681 gtggacgaca ttacccgtgc tttatttagc gccatgctac tggtggcaaa atcaaccaga
    10741 atcggcggac gcccatcact gaacggccag gcaaagcaga aagggttagt ggtcatacgc
    10801 ggttccgcac cgccccacgg ggcgaccagt gcaggacgcc cggtgacgtt actcatggcc
    10861 agcatcgcca ggccttgatc agcaaccatt tgcccgtaat gccccatacg ccctaaatga
    10921 tgcgcatggc acaacgttaa caggcaaaaa ccgcggtgtt ttgcttgttc gatggcagca
    10981 tttaccgcca cttttccggc gtgttgacca aagccattgt gggcatgaaa acgttgtaaa
    11041 ttaccttcac ttgttaagca ttccgggcgg gcattcgcgg ttacatcgcc acgagcgata
    11101 ttttccagat atttcggtag cagcgtgacg ccgtggctgg caaagccata acgttccgct
    11161 tcaatcatat gttccgccac atcctgagcg atatcggcat cacagccatt aagttccagc
    11221 agcccggcag ctaaccaacg cactttttca acagcaattt gtaatgacat cgttggtttc
    11281 ctcacattag acctaaaatt ttccaccagg tggcggcaaa cagtagcaac aacaggtagc
    11341 cgatcaccgt caggtacagt cccacacggg taaactggcg tggcgtgaag gtatccgtac
    11401 ccatacacac catattttgt ggcgcgttaa tcggcaggat gaagccaaaa ctgacactga
    11461 aggcgagcag aatggtcatg ccgaccgggt taacgccgag ttctggcggc agactgctta
    11521 acaggctaat caggatgggt agcaatgcgg cagttagcgc agtggcactg gcaaacccca
    11581 ggtgaatgat tatcaagaaa gcggcgagaa tcgcgaagat ggctaatgat ggcaagccat
    11641 cgagaccaaa acctttcacc acatagttcg ccatccatga agccgcctgt gtatcaagca
    11701 acgtggaccc caggctgata ccaataccaa acatcagcaa cgtgccccac tgcacacgtt
    11761 tttcgacctc tttccagttc attacaccga tgcctggcag caacataatc gccagtccgg
    11821 caagggtgac agaggtggta tcaatactgt gtaattttcc gccagtagac caaaagagca
    11881 gtaacaatag cgaaataccg atcaagcgtt tttctttacc ggtggttggc cctaattctg
    11941 cgagtgcttt tttaatggcc tcgctccccc cttcaacagc ttccgtttct ggcggcaaca
    12001 atttacgtgc gaggaaatag aggatcaccg acatggtgag gctccagggc gcgcccgcta
    12061 acagccagtc aagccagctg acagaatgcc cggcgccgaa agttttattg ataaaaccga
    12121 tagaaagcag gttttgcgcc gccgaagttt gaataccaac gttccagata ctggtcgcct
    12181 gggcgataac aatcatcatt gacgccgcta aacgtgaatg tttatccacc ttaaatgccg
    12241 caatgacccc catcataatc ggcaccacgc aagccgtacg ggcagtggca ctgggtacga
    12301 ccaggctcag gacgatggtc accacaatgg caccgataat aatgctgcgg ctgctggcac
    12361 ctattttcga catagtaaac agcgcaatgc gtttatccag cccggtaatg gtcattgccg
    12421 ccgcgataaa cattgctgct gccaccagcg ccagagcaga attagaaaaa cccgacagag
    12481 tcattttcag cgctttcgcg gtgccaagga tcgtgtccgg gtgattcata tccggggcaa
    12541 aaccaaccat aaagataatc agtgcggaaa taacaattgc actggcggtg taatccatcg
    12601 cttcgctgat ccagacaata atggcaaaca ccagaatggc gatcatattt tgtccggcga
    12661 cgggcaaggt atcgcccaga ggcagaagag taataataat tccggcaatg atcgccaacc
    12721 acaggccggg attagggata attcgacgct tttgtggtgc gccatgtgat attgatgaca
    12781 tattcacctc attgtgaaaa ataaaaaacc tgcctggcta ttgcaggagc tatgccatct
    12841 ttaatttttt aataacgctt tgttatttaa tgattttatt atttattctt tttattttgt
    12901 gcgcggtttt ccgcacagta aataaaaggt gattctctta ccacacagat aaaaaaaggg
    12961 agcgatatgc tcccttgata ttcatttatg gattacttaa taacattcca taacgtttcg
    13021 ccaagtaatg ctggatttcg tgtccatgcc atgcctgctt tatcgaacgc ccggaattta
    13081 tcctccgcac tgccgctacc gccagaaata atggctccag cgtgtcccat gcgttttcct
    13141 tttggtgcag tgacaccggc aatataacct acgaccggtt tattcatttc atggcgaata
    13201 aattccgcgg cccgttcttc cgcattacca ccaatttcac cgatcataat taccgcgtct
    13261 gtttgcggat cattttcgaa taaacgcagc gcatcaataa agttagtccc cggaaccgga
    13321 tcaccgccaa taccaataca ggtggattgc ccaagtccca gcgccgtggt ttgcgctaca
    13381 gcttcgtagg ttaatgtacc ggagcgggaa acaatcccaa tacgccctgg gcggtggatt
    13441 tcaccaggca taatcccgat tttgcactca cccggcgtga taactcctgg gcagtttggc
    13501 ccaatcagcc gcacatccgg gtggcagtca agcagccgac gaactttcac catatccagt
    13561 gtcggtacac cttcagtgat caccacaacc aatttgacgc cagcttcaat agcctcaaca
    13621 acagaatcca gcacaaacgg tgcaggcaca taaatcacgc tggcatcagc ctcagtttgt
    13681 cgcatcgctt ctttcattga atcaaaaacc gggcgatcca gatgccgttg cccgccctta
    13741 cctggcgtca ctccacccac aatctgcgtg ccatacgcaa tagcctgctc tgtatgaaat
    13801 gtgccgtttt tgccggtaat gccctgcacc agtacccgcg tgtgtttgtt gatcaaaacg
    13861 ctcatcttaa cctcctgtta attcagtaac gcaatgatgc gtttcgcggc atcatccaga
    13921 gaatcgacgg cttccaccgt caggccactt tctgccagta accgctgccc ttcggcagcg
    13981 ttattaccgg agagacgcac cacgacaggc agggtgatac gggcctcatt cagagcatga
    14041 ataatggctc tggcgatcat gtcgcaacgg acaatcccac caaaaatatt aaccagaatg
    14101 gctttcactt tgctgtcgga aacaatcagc cgaaaggctt cactgacgcg ctcctgggtt
    14161 gcgccaccgc caacatccag gaagtttgcg ggttgttcac cgtatagctt gatgatgtcc
    14221 atggttgcca tagccagccc tgcaccattg accatgcagc cgatatttcc gtccagcgag
    14281 acataattaa gatcaagctt cgctgcctgg ctttcgcgtg gatcttcctg ggtttcatca
    14341 cgtagcacct ggagttccgg gtgacgatac agcgcgttat catccagcga tactttcgcg
    14401 tctgcacaca taaattcgcc cgtttcccgc aacaccagag ggttaatttc cagcagggcg
    14461 aaatccagtt cgttaaaggc tttccacgcc tgattaacca ggcgactaaa ggcagcaaat
    14521 agtccgtgtt ccagttgcag aacagcaaac atttcgcgga tatggcaagg ctgcacgcct
    14581 gtcagtggat caatgctgac actgctgatt ttctccggtg tctcatgggc gactttttca
    14641 atttccacac caccttccgg gctgacaata aacgtgaccc gctggctttc acgatccacc
    14701 accatgccaa aatagagttc ctggcgtacc ggataaatgt tttcgcacag taaaatactg
    14761 ctgacatact gtccttctgg cccggtctga taagtcacca gttgcgatcc caacatttgc
    14821 tgtacaaaag cctgtgcttc cggcagttgc ttaagcactt ttacgccgcc agctttaccg
    14881 cgcccaccag catgtacctg cgctttaagt accgcgcctt tactcgggca agcaatatgt
    14941 tgccaggcat ctgccagttg cgaaatctgt tggatggcta tctcttttgg acacggcatc
    15001 cccatgccag ccagtaacga tttggcctga tactcatgta aattcatata ttccctcagt
    15061 ggtatgtgtt atccgtgtaa tgctttatta ttcaaggcca ttgctgcttc gtggatgact
    15121 tcgcttaatg tcggatgcgc atggatcgca caggcaatat cctcgccaga ggcgctaaaa
    15181 gtcatcgcca gcgcaatttc attaatcagc tccgatgcct gcgggccaac aatcgctccg
    15241 ccaagtacgc ggtcagtgtg tttatcactg tataacgtgc aacgcccacc ttcctggcct
    15301 aatgctaagg cgcgaccgtt accggcaaac agagaatttc ctttgttaaa gactctgcca
    15361 gcggctttca gtgaggcctc gttttcccct acccacgcaa cttccggttg ggtgtaaatc
    15421 accgaaggaa tcagcgcaaa attaatcggc tctaccgcca gtccggcaat ttgatcagca
    15481 accaccacac cttcagccat cgctttatgc gccagcatcg gccctcgcac gacatcacca
    15541 attgcccaca gcccggcttt cccggtacgg cataagttat cgacggcaat accgccacgg
    15601 ttatcggctt caagaccgag ttgcacgaga tcgacaccag aaagtcgcgg tacacgtcca
    15661 atggcaagaa ttaatttatc gaagcggctt tcttctcgct tctcaccctg ccgccagcgc
    15721 acatgcacac cgtcatcccg ttgttcaata gcctcaatct ctactgccag ttgcattttc
    15781 atcccactgg caatcattgc tttacggact tcgttcgaca ggcgagcttc cagtgcaggc
    15841 aagaacgttg gcgccatctc cagcaacgtg acgtctgaac cgacccgatt ccacacagac
    15901 cccagttcaa ggccaatcac gccagcgccg atcacaccga gacgcggcgg cacagcggtt
    15961 aacgccaggg cgccgcgatt atcgagaatt tgctgattat caatggtaac gccaggcagt
    16021 tgacgcggct gagaacctgt tgcaatcacc acattacggg cgcggatttg ttgatcgttt
    16081 acacgtaatt gccaaatctc atcccgagca tgttctaacg ttgcgaggcc gcacaggtac
    16141 ttcactttat ttttcttaaa cagcaagccg atgcccattg tcaggcggct tactatggcg
    16201 tctttacgtt gaatcatcgc agctgcattg aaagatacgc cgtcaacatt aacgccatga
    16261 atgctcgcct catgctgcac ttgcgcgtac agttcggaag attgcagcaa cgattttgac
    16321 ggaatacagc cgacattgag acacgtaccg cccggcgaag gttcgccctg tgcattaaca
    16381 ccatcatcaa tacatactac ggaaagccca ttctgcgcag cccgcagtgc cgctacgtaa
    16441 ccacctggcc caccccccat aactgccaca tcaaaaattg tactcatcgg cgccctcctt
    16501 aaagatccag caacagttgt tccggtgatt ccagtagctc gcgtattgcc accagcgtct
    16561 gcacggcttc ctgaccatcg ataatgcgat ggtcatagct gagagcaagg tacatcatgg
    16621 gacgaataac aacctggccg ttttctgcga ccgggcgcgg cgtgatggca tgcatcccaa
    16681 gaatggccga ctgcggcggg ttaatgattg gcgttgacat catcgaacca aaagtaccgc
    16741 cgttggtgat agagaacgtg ccgccctgta acgcctccag cggcaatttg ccattgcgtg
    16801 cctgtgtggc gtattcggca atttgccgtt caatttccac caaagagagt gattgcgcat
    16861 tgcgtaacac aggcactacc aggccgcggt tgctgctcac tgcaatgccg atatcgcaat
    16921 aatcacgcca gataatctcg ttgccatcaa cgctggcatt cactacaggg aagcgctcca
    16981 gcgcccgggt aacggcctta acaaagaagg acataaagcc cagcttcacg ccgtgcttct
    17041 cggcaaaacg atctttccag cgggcgcgta aatccatcac gctctgcatg tttacttcgt
    17101 tgaatgtggt aagaatggcg ttattttgtt gggaagccag cagacgctca gcaatccgct
    17161 gacgtaagcg cgacatcggc tcacgacgtt cctgacgagc acccggtgtc aagggtttag
    17221 ctggcgcgat ctccgccacc cgttcaggct gaatgactgg tgcaggagta acgcgctgta
    17281 catcctcttt caggatacgt ccatttctcc cgctccccgc aacgtcagca agttcgacac
    17341 cactacgctg tgcctccagc cgtgcggaag gcatcgccag ggtttcggtg actggtgtga
    17401 cggtttcttc tatgacagct tgcggcttaa gatgcgccaa cagttgcgcg gacgtgaccg
    17461 tgctaccttc gctgacgata atattgctta acacgccatc gtgcggggcc ggtatttcca
    17521 gaatgacttt atcggtttcc agctcggcaa tcacatcatc gcgttttacg tgctcgcctt
    17581 cttgtttaca ccaggttgtc agcgtgcctt ccgtgacaga ttccggtaat acagggacag
    17641 taatttcgat cataaaatgt tctccttatt atttttgtgc gcctttcatg gctgaatatc
    17701 tgcaaacgca gcagcaagaa attcgtcgat ttgttgttta tgtacacgcc cataccctgt
    17761 cgccggagct gccgctgccg ggcggcctgc atactgccag taaggagtgt taattttcag
    17821 tgccgccagc tcatgacgaa tctgccgcca cgcaccctgg ttttctggct cttcctgcaa
    17881 ccaaatccat tcgcagcagt tcggccaact cgccagaaca tcattcagtt ctgcgaccgg
    17941 gaacgggtag agttgctcca ggcgaacaat cgctacctca tcttcacact cacgctgctt
    18001 acgggcttcc agtacgtcgt aatagacctg gccgctacac aaaataacgc gcttaacttt
    18061 ctgggactga tgcaattgcg gatcggtgac taccggttta taagcgccat cagtgaattc
    18121 agaaagctcg ctcatagcgc cttaaaccga agtaaacgct tactcatcat tatcactaac
    18181 ggtttgcgca ttgggcgtaa tgcctgaccg cgcagcaaat ggaacatttg tgctgactca
    18241 ctgggcatca ccacctgcat gttgttttct gcgcacagtt gcagccagcg ctcaggacgc
    18301 gcagaagagt gctccggccc ttgtccgtca tagccatgcg gcagaagaat ggttaaaccg
    18361 ctatagcgat cccacttggt ttcgccagac gagataaact ggtcaatagc aacctgagcg
    18421 ccgttggcaa agtcaccaaa ctgcgcttcc caaataacca gttgctgagg cgcagacgtt
    18481 gaatagccat attcaaaggc taagagcgct tcttcattaa gcaccgaatc ataaacatcg
    18541 aaagatgcct gaccagcgcg gatatgctgt aacggcagat aacgacgggc ttcagtttga
    18601 tgatgtacca ctgcatggcg atgactgaac gtgccacgcc ctgagtcttc accagaaaga
    18661 cgcacaccaa cacccgcatc cacaagcgat gcataggcca gcatttcagc cattccccag
    18721 tctattggct gagtacctgc ggccatatcc tggcgtaacg ccagttgccg tttaatggta
    18781 ggatgtgcga ccacatcggg tggcagtgta gaaatgattt caccatacgc tgccagcttt
    18841 tgccgaggca atgccgtaga taccggggcg ctccagtgag ggttggtcaa cccataccag
    18901 ttagcgctaa aagagtgaat tgcaggtttg agaggctgtg gttcacgttt ctggcagcta
    18961 tccagccagt cgcgatagcg tgccgtcatc tcatcttgtt gcgcctgagt taacaggccg
    19021 cggcgggcaa gagattcacc ataacgggcg agcgtaccag ggtgagcatc cactgcctga
    19081 tacatttgtg gccgggtaag acgtggctca tcgctttcgt tgtgtccgtg cttacggaag
    19141 cagcaaatat cgatgatgat gtcgcgccgg aaggtgtctc gccattcgca caccagttcc
    19201 attacctggc aaacagcgtc aacgtcatcg ccattaacgt ggataattgg ggccgctacc
    19261 attttggcaa tatcggtgca gtaccgtgaa gaacgcatat cctgcaagcg ggaagtggtg
    19321 aagccaatct gattgttaat caccagatgt agcgtaccgc cagtgccgta gccctgagtc
    19381 tgcgataagt taaacgtggt ttggttgacg ccgaggccgc ccaacgcgga atccccatga
    19441 ataagtacgc ccaccacttt tgcctgacca tcctcaccac ggcgttcctg cctggctcgc
    19501 acctggccca gcaccaccgg attcacgatc tccagatgtg aggggttata cgccagcgcc
    19561 acatgcagcg aaccggcagg tgtttccaga ttgctggaat accccatgtg gtatttcaca
    19621 tcgcctgaac cactgccgat cgtctgcttg ccttcaaact cagcaaaaag ctgtgccggg
    19681 tctttattca acagattaac cagaacatta agtcttcccc ggtgcgccat gccaatgacc
    19741 atttcctcca caccttgcgc acgcaggcgt tttgtcagcg tatcgagcgc cgggattgca
    19801 ctttcgcccc cttccagaga aaaccgtttc tgcccaacat aacgggtatg cagataacgc
    19861 tctaacgtct ctgccgccat cagtttttca aaacgagcaa tacaggtttg tacatctgct
    19921 tgtggcgcat tgctgctctc aatccgtgat aacaaccagt tgatttcttc acgattttcc
    19981 agatgagcaa gttcataagc ctggctaccc gcccaggctt gctccagcag gttaagcagt
    20041 tgcttaagtg gcatttgcgt ggtgtgtgcg ccaaaggtga cgctaaattc ctgcaacaag
    20101 tcttcttcgc ttaatcccca aaaaccaggc tggagggagg gaacgtcagc cgggggattc
    20161 aaaccaagtg gatcaagctt tgcgcgcaga tgcccctgcg tgcgccaggc attgatcagt
    20221 tgtattaccg cagcctgttt ttttaacgtc gcaccgctga cgttgttatc accggatacg
    20281 ctttggacag tcagttcagg tgaaagaaaa aaacgccgcc agtcttccgg gagtttttcc
    20341 ggcgtacgca accaggtctg gtagtactct tcaagccagg tcgcgttatc tccactaagc
    20401 agcgtgccgg gcgaggtcat gttctccatg tttttctcct gatggtcata gacagccgca
    20461 ttactgcaca atccgtacca acagttaaaa acactaattt tcaaaaactt atagaaaata
    20521 gatgatgtaa aagtgggcga aaaaagggac agaatagtag gggatggctg gagtcaggaa
    20581 caggcggaga gatccgcctg ttgattaaaa tcacatcacc gcagcaaacg cctttgccac
    20641 gcgatgcaca ttttgcgcgt taagcccggc gacacacatg cgaccgctgg cgatgagata
    20701 gacaccaaat tcttcacgta gtcggtcaac ctgagcgatt cttaaaccgg tataactgaa
    20761 catgccgcgc tgattaagca gataatcgaa attgcgctct ggcatctctg tgctcaatac
    20821 cttcaccagt tcctgacgca ttgccagaat gcgagttcgc atctcttcta cttccgccag
    20881 ccagccggct ttcaatgcct cgtcattcag caccgcagcc accaccttcg caccaaaatt
    20941 cggcgggctg gagtagttgc ggcgaacggt tgctttcaat tgccccagta cgcggcctgc
    21001 ggcttcggca tcttcacaca gaacagaaag tccgccgacg cgctcgccgt aaagggagaa
    21061 aattttcgag aacgaattgc tcaccagagc gggtaatcca gcgctggcaa tggcgcgaat
    21121 ggcgtaggaa tcctcttcca taccggcacc aaatccttga taggcaatat cgaggaatgg
    21181 aataagctcg cgggctttga gaatttcaat cacggaatcc cactggtcat tagtgagatc
    21241 ggcacccgtt gggttgtggc aacatggatg cagcaacaca atactgcggg caggtaatgt
    21301 tttcagcgtc gccaacaggt cattaaagcg cacgccgtta gtcgcttcgt cataccaggg
    21361 gtaagtgctc acttcgaatc cagccccggc gaatattgct acgtggtttt cccaggtagg
    21421 atcgctgacc cagacgcctg attccgggaa gtagcgtttc aggaaatctg cacccacttt
    21481 caatgcccct gagccgccaa gggtttgaat ggttgctacg cgctgttgtt gcagtaccgg
    21541 atggtccgca ccaaacagca gcggcgcaat ggcatggcga tagctgttaa gcccttccat
    21601 cggtaaataa agcgaagcac catgaggctg cgcattcagg cgcgcttccg catccgccac
    21661 ggctttcagt tgtggaataa ttccgtcttc gttgtagtac agaccgatac ttaaattcac
    21721 tttgtcgctg cgagggtctt ctttaaaacg ctccataagc gtaagaatcg ggtcgccagc
    21781 gtaggcgtca actttttgaa acacgcgatg gttctccagg tttacgggca ggtggttaaa
    21841 acacaataaa ccggaagtag gcgaagatcg agaggatgtt caggggcgaa tggcaattag
    21901 caacagagtg agactcatga caaacgtaca tccgccagat gcacgacctt tataagagca
    21961 tgaaagaata tcaactcatt gaattggtaa gattttattg gccggataag gcattcacgc
    22021 cgcatccggc acagacaatc aaatattaca gaacgattaa tccacgtatt tcatcgcgac
    22081 tcttgaggtc aggcgcgtga taagttcgta agcacttact ttcgtcattt cagcgatacg
    22141 ttctacgggc aaaccttcgc cccataaaat gaccgggtcc ccggctttat cctgcgcctg
    22201 tggacctaag tctacgcaga tcatatccat cgccacgcgc ccgacaatcg gcacttcgcg
    22261 accgttcacc agcactggcg taccggacgg cgcggcgcgc ggataaccat cgccatagcc
    22321 catcgcgact acgccaagac gagtatcacg ttcgcttacc caggttccac cataaccgac
    22381 aggctctccg gctttatgct cacgcacggc aatcaggctg gaggttagcg acatcactgg
    22441 ctgacagcca aaatcggcac cggtggagcg atcttcaagc ggagatacgc cgtaaagaat
    22501 gatgcccggg cgcacccagt caaaatgcga ctgtggccag agtaaaatgc cacccgacgc
    22561 ggcaatggaa cgttgaccag gtttgccttc gcaaaaggta ttaaagatag cgagttgctt
    22621 ctcggttgcg ccgcattttg gttcatccgc gcgcgcaaaa tggctgacga tattcaccgg
    22681 ctgacgaacg tttttgcact gggtcagacg atgataaaac gcctcagcct gttccggcct
    22741 tacgcccaga cggtgcatac cggtatcgag tttcatccag acggtaaccg gctcgtccag
    22801 gctagcctct tccagcgcag ccagctgttc ttcgttatgc acggcggtat gaaaatgttg
    22861 cgcagaaatc gtcggtagat ctctggcatc aaaaaagcct tcgagtaaaa gtacaggttt
    22921 ggtgattccc cccgcacgca gtcgcagagc ttcttcgaga cgggctacgc caaaggcgtc
    22981 agcatcgggg agcgttcgcg cggtctcaag aagaccgtga ccataagcgt tcgctttcac
    23041 caccgcaacc attttactgg caggcgccag ttcacgaaga cgttgcaggt tgtgtcgcag
    23101 agcgcggcgg ttaatcacaa cagttgccgc ttgcatttgt gttccttgat aagtgtttgc
    23161 tttaattacc taattcataa aataattatt attcgtcgtc gtactgcggc cccgcatagt
    23221 tgtcgaagcg cgaccattga ccgttaaagg tcaggcgtac cgtcccgatt gggccgttac
    23281 gttgtttacc gataataatt tccgcgatgc cttttaaatc actgttttcg tgatacacct
    23341 catcacgata gataaacatg atcaagtccg catcctgctc gatagagcca gattcacgca
    23401 ggtcggagtt gaccgggcgt ttgtcggcac gttgttccag agaacggttc aactgggaca
    23461 gcgccaccac cggcacgttc agttctttcg ccagtgcttt cagcgagcgg gagatttctg
    23521 caatttccag cgtacggtta tcggaaagcg ccggtacgcg catcagttgc aggtagtcga
    23581 tcatgataag cccgatgccg ccgtgttcac gggcaatacg gcgtgcgcgg gaacgcactt
    23641 ccgttggcgt caagccggag gaatcatcga tatagatatt acgtttttcg agcaaaatac
    23701 ccatggtgcc ggaaatgcgt gcccagtctt catcatcgag ctgaccggta cggattttag
    23761 tctggtcaac gcgcgacagc gacgccagag aacgcatcat gatctgttct gatggcatct
    23821 ccagcgagaa gataagcacc ggtttatcct gcaacatcgc cgcgttttcg acgaggttca
    23881 tcgcaaatgt cgttttaccc atcgacggac gcgcggcaac gatgatcaaa tccgacggct
    23941 gcaagccagc ggtttttttg ttgagatcgt cataaccggt atttaccccg gtaacgccat
    24001 cgtgtggctg ctgaaacaac tgctcaatac gcgccacggt tgcgtcgagc acatcggcga
    24061 tgttcttcgg cccttcgtct ttgtttgcac gactttcggc aattttaaag acgcgggatt
    24121 cagcaaggtc gagcagatct tcactggtac gcccttgtgg atcaaaacca gcttcggcaa
    24181 tctcgttagc aacggagatc atctcacgaa caacggcacg ttcgcgcacg atatccgcat
    24241 atgcactgat gttcgccgca cttggcgtat tttttgacag ctctgccaga taagcaaaac
    24301 caccgacgct atcgagttgc ccctggcgtt ccagcgattc cgcaagggta atcagatcga
    24361 taggactacc gctttcctgc aaacgcgcca tttcagtaaa gatatgacgg tgtgggcggg
    24421 tgtaaaaatc gtctgccacc acacgctcgg ctacatcatc ccagcgttcg ttatccagca
    24481 ttaaaccgcc caacaccgac tgctccgctt cgatcgagtg cggaggcact tttagcccgg
    24541 caacttgtgg atcgcgttcg cggggttcag cctgctgttt gttgaagggt ttatttcctg
    24601 ccatagtgaa tggagttacc gagataaaga atgggtcgaa actttaccat atgaagcaga
    24661 ccctgacgat acgttctgga ggacacatgg caacacgaat tgaatttcac aagcacggtg
    24721 gcccggaagt acttcaagcc gtagagttca ctcctgccga tccggcggag aatgaaatcc
    24781 aggtcgaaaa taaagccatc ggcatcaatt ttatcgacac gtatatccgc agcggccttt
    24841 acccgccacc atcgctaccc agcggattag gcaccgaagc ggcaggcatc gtgagtaaag
    24901 tcggcagtgg tgtaaagcat attaaggcag gcgatcgcgt agtctatgca caatcggcat
    24961 taggcgctta cagctctgtg cataacatta atgcggataa agcggcgatt ctgcctgcgg
    25021 caatttcttt tgagcaagct gcggcatcct tcctgaaagg cttaacggtt tattatctgc
    25081 tgcgcaaaac ctatgaaatt aaacccgatg agcagttcct gttccacgca gcggctggcg
    25141 gcgttggctt gattgcctgc cagtgggcaa aagccctggg cgcgaaactt atcggcaccg
    25201 tgggaaccgc gcaaaaagcg cagagtgcgc taaaagcggg cgcatggcag gttattaact
    25261 atcgtgaaga gaatctggtc gagcggttaa aagagatcac cggtggcaag aaagtgcgcg
    25321 tggtgtacga ttccgtgggc agagacacct gggaacggtc gctggattgc ctgcaacgcc
    25381 gcggcttaat ggtgagcttt ggcaactcat caggtgcggt taccggtgtg aacttaggca
    25441 ttctcaatca gaaaggctcg ttgtatgtga cacgcccttc cctgcaaggc tatatcacca
    25501 cgcgggagga attaaccgag gccagtaatg aactgttctc tttgattgcc agcggtgtga
    25561 ttaaggtcga tgtcgccgag cagcagaaat atccgctgaa ggatgcgcgg cgtgcgcatg
    25621 agattctgga aagccgggcg actcagggtt ccagcctgtt gattccataa aagaaattgg
    25681 gcttccacct gggaagccct ttcttttata gttcggctgt atgtagggta cagctcgatg
    25741 aatttgtttg acgcgcaata gtgacagatt tgattatcaa ttcctatttt gttctaagga
    25801 taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg tcataacgct
    25861 gatatttcgg cacttttggt gctttaatcg ccctgatcac ccacaccacc gcaaccgcca
    25921 acagcagcca cggcagcaac ttaatcatca atgccagcat accgccgagg aacataaagg
    25981 ccgtcgccac aatcagcgcg gcgataatac ccagcaacga aacaccggtg accatcagca
    26041 tgacaaaaaa gccaattaca aaaagtagtt ccagcatgat gctctcccaa atatgaaatc
    26101 tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca gcaaaacgcc
    26161 ccgcgacggg gcgcagggcg tggtgaattt gactactttt tggtgaaaag ttaacgctta
    26221 tccgccacca gtttgagcgc gtgttccagc acattaatgt ctgcacccgc tttatgggca
    26281 ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa caagcccagc
    26341 atatgccggg taatatggcc gagatacgta ccctggctga gttcacgctc aatgtacgga
    26401 tacatggcgc gcactaccgc caccggatca gcatcagtat ccgaggaacc aaaaatctct
    26461 cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac catcacgcca
    26521 tccatatgtt gcaaatgcgc tttagcttct tccagcgact tgataccacc gttaatcgac
    26581 atcgtcagat gcggaaagtc acgcttcagt tgatacacac gcggataatc gagcggcggg
    26641 atctcacggt tttctttcgg acttaaccca gaaagccagg ctttacgtgc gtggatgatg
    26701 aacatctcac actcaccttt gccggaaacg gtgttgatga aatcgcagag aaattcatag
    26761 ctgtcctgat catcgatgcc aatacgcgtt ttcaccgtca ccggaatcga caccacatcg
    26821 cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca cgcaccaaac
    26881 atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc atcatatccg
    26941 cgcgcttctg ccagcttcgc acactgtgcc agcgccgccg gatcgctacc cccgagttgc
    27001 aacgctaccg gatgttcttc ttcactgtac gccaggtaat cacctttacc gtgaataatc
    27061 gcccctgtgg tcaccatttc ggtatacagc aacgtattgc gggaaagcag acgcaggaaa
    27121 taacggcaat gtctgtccgt ccagtcaagc atcggggcaa tcgagaaacg gtaagcggga
    27181 taaacagtgt gtgattctgc tggcatggcg actttttaag catctggtaa attgggggcg
    27241 ctactatagc ataacgaata tgcgatcagg caatgtgaca gccatgacac cctgttctcg
    27301 gcgtttaagc gagaactatg gtaaagtaag ggcattctta accccactgt cgaggtgccc
    27361 aatggaaaag accacaacgc aggagttatt agcgcaggct gaaaaaatct gcgcacagcg
    27421 taatgtgcgc ctgaccccac agcgtctcga agtgttgcgc ctgatgagtc tgcaggatgg
    27481 cgctatcagc gcttatgatc tgcttgattt gctgcgcgaa gctgaaccgc aagccaagcc
    27541 gccaacggtt tatcgcgcgc tggagtttct gcttgaacaa ggttttgtgc ataaggtgga
    27601 atccaccaac agttatgtgc tctgtcatct gttcgatcag cccacccata cttcagccat
    27661 gtttatttgc gatcgctgcg gcgcggtgaa agaagaatgt gctgaaggcg tggaagacat
    27721 catgcatacg ctggcggcaa aaatgggttt tgccctgcgg cataatgtta ttgaagcaca
    27781 tggattatgt tcagcatgtg tagaagtaga agcgtgtcgt catcccgaac agtgccatca
    27841 tgaccactct attcaggtga aaaagaaacc tcgctaaaag ggtgtacatc cttgtacatg
    27901 tcgggcagga gggattaatt accagcggta atcatggcgt ttttcccaac tatcaacctc
    27961 tttttctgcc tgatctttct gataaccgta gcgttcctgg attttaccca ctaactgatc
    28021 acgttttcct tcaatgatcg tcatatcatc atcggtcagt ttgccccatt gctctttcac
    28081 tttaccttta aactgtttcc agttaccgcc ggcttcatct ttattcataa tcaagacctc
    28141 atcgttaggt tgtgaatgag agtacgttca cttttcttct gaacgtgaga ttaagtgtag
    28201 tcagcaattt ggcttaggat tatttattca gaatttttaa ccgtcacgtt gcgacaaacc
    28261 aggtatcgtt acgccagtga cgccgccaga tagccgccag agaaagcccg cgtaacgcca
    28321 gaaagacggt taacgccagc cacaaaccat gattccccag ccacggcagc gtaaggagcg
    28381 tcagtgcaaa acctgcggcg gccaccgcca tactgttacg catttcggcg gcacgcgttg
    28441 cgcctataaa catgccgtcc agcagataac accagacgcc gaccaacggc aaaatcacct
    28501 gccagataag atagcggtca gccagctgct gaatctgggt taacgacgtc agcaacgcaa
    28561 tgatgtgttc ccctgccagc aaataaacca ccgaaaacag taacgctacg atccccgact
    28621 ggcggcacgc tgcccgccag acatccagca attgactacc gtcgcgcgca ccgtatgcct
    28681 gaccggaatg tgcttcaacc gcgtaggcaa aaccatccag cgcataggcg gtaaaggtga
    28741 gtagcgtcat cagaactgcg ttaacagcga taatgtcact ccccagtcgc gcgccaagta
    28801 cagtgatcgc gccgaaacag agttgcagca acagcgagcg cagcatgata tcgcggttaa
    28861 gcgccagcaa gtgacggaag tttcctcgcc aggctgtttt cagcatttcg ccggagattc
    28921 cgcgtagttt gagaatttta cgcaccatta gcagaccaat cagcaatgtt gcatattccg
    28981 caataaccgt cgccagagcc gcgccctgca cgttcatatg cagccccatc accagccaga
    29041 catccagcac aatgttgagg atattgccga ccactaacaa aattactggc gcacgggcat
    29101 attgcacgcc gagtaaccag ccaagtaata ccagattcgc cagcgacgcc ggtgcgctta
    29161 accagcggat ttcaagaaag cgtcgcgcct gttccaggac tgcttcacta ccaccaacaa
    29221 tatgcagcgc cagatcgata atcggcgtac gcagcagcgc aattaacgct ccggccccca
    29281 acgccaacag caacggttgc accagcgcac gggctaatgc ctgaggattt ttggcaccat
    29341 aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa cagcaacaac ataaagagaa
    29401 agctggtcgc cgttgcacca accgccacgc cgcccaaata aaccggacta tcaagatgac
    29461 caattaccgc cgtatcgacc agtcccagca acggaacggt gatattggag aaaatcatgg
    29521 gtaaggcgag atgccagagt gctttatcag atgaagtgag gaatgccatg cagacaagcc
    29581 tgatgaagag agatgaaaaa caaaccgcga cgccaggcgg catcgcggtc tcagagatat
    29641 gttacagcca gtcgccgttg cgaataaccc caaccgccag cccttcaatg gtgaagctct
    29701 gctgacgaag gtcaacgaca attggtttaa actcgctgtt ttctggtaac agttcgactt
    29761 tattgccctg ttttttcagg cgtttaacgg taacttcgtc atcaatacgt gcgacaacga
    29821 cctgaccgtt acgtacatcc tgagttttat gcactgccag caaatcgcca tccataatgc
    29881 cgatatcttt catcgacatc ccgctgacgc gcagcaggaa atcagcattc ggcttgaaca
    29941 aggaaggatc gacctgataa tgaccttcga tatgctgttg cgccagaagc ggttcaccgg
    30001 cagccacacg acctaccagc ggcaaccctt cttcctcttc ctgcaataaa cgaatcccgc
    30061 gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc cagcgccttc agatgttctt
    30121 cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat ttccgcacgc gtcggcggca
    30181 tacctgtctg gctgatgtga tcacggatga gatcaaacac ctcttgttgc ctggccgtta
    30241 acgctttcat tccgccccct gggtgtatat acagttatgc tgtgagtata tacagcaaaa
    30301 ggcgattttg gaaccataaa ctgcacaata aaccagagat ttatcgaaat ctggaagggt
    30361 tatccaaaat gcgaccataa caggatgcac caggtaatca cggcgacgat aatggcaatc
    30421 agcactgcgg cggatcccat atctttagcg cgtccggaaa gctcatggta ttcagagcca
    30481 attcggtcaa ccacagcttc gatggcgcta ttgagaattt ccacaatcat caccagcatc
    30541 acagagctga taagcagcac gcgggtaatc gcgtccacat ccagccagca ggcgataacc
    30601 accgctaaca ataccgctac gccttcctga cggaatgccg cttcgttgat ccatgcagcg
    30661 cgtaaacctt tccaggaata gccagcagct ttgataattc gggtgaatcc agtggtatta
    30721 ttggccattg aaagaacctt tttacattat gagcgtcaat atcagtgtac cgttaaatcc
    30781 gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg gataccacag aatttcccat
    30841 gactttctgc tatccttgcc gcgcatttgc attattaacc agaggcttta catcgtttat
    30901 gtccggctgg ccacgaattt actacaaatt actgaattta ccattaagca tcctggtaaa
    30961 aagcaagtct attccggcgg atcctgcccc ggaactgggg ctggatacct ctcgtccaat
    31021 tatgtacgtt ttaccgtaca actcgaaagc tgacttgctg acgttgcgcg cccagtgtct
    31081 ggcacatgac ctgcctgacc cgttagagcc gctggaaatc gacggcacgc tactgccgcg
    31141 ctatgtgttc attcacggcg ggccgcgtgt gttcacctat tacacgccga aagaagagtc
    31201 tattaagctg ttccacgact atctcgattt gcaccgcagc aacccaaatc tggatgtgca
    31261 gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg cgtgaaaaag gcgaagtgaa
    31321 cccgccgctg cgtatgctta acggtgtaca gaaatttttc gctgtactgt ggctcggtcg
    31381 cgacagtttt gtgcgtttct cgccgtcagt ttcgctgcgc cgtatggcgg atgaacacgg
    31441 cacggataaa actatcgctc agaaactggc gcgcgtagca cgtatgcact ttgcccgtca
    31501 acgtctggct gccgtgggtc cacgtctgcc tgctcgtcag gatctgttta ataagctgct
    31561 cgcctcccgc gccattgcga aagcggtaga agatgaagca cgcagcaaaa aaatctccca
    31621 tgaaaaagcg cagcagaacg cgattgcgct gatggaagag attgcggcga atttctctta
    31681 cgagatgatc cgcctgactg accgcattct gggcttcacc tggaaccgac tttaccaggg
    31741 tatcaacgtc cataacgccg agcgcgttcg ccaactggcc cacgacggcc atgagctagt
    31801 gtatgtgcct tgccaccgca gtcacatgga ctacctgctg ctttcttacg tgctgtatca
    31861 ccaggggctg gtgccgccgc atatcgccgc cgggatcaac ctgaacttct ggccggctgg
    31921 gccgattttc cgccgtctgg gggcgttctt tattcgccgt acgtttaaag gcaataaact
    31981 ttattccacc gttttccgtg agtatctcgg cgaactgttc agccgtggtt attccgtcga
    32041 gtatttcgtg gaaggcggtc gttcccgtac ggggcgtttg ctggatccga aaaccggtac
    32101 gctgtcgatg accattcagg cgatgctgcg tggcggcact cgtccgatta cgctgattcc
    32161 gatctatatc ggttatgagc acgtcatgga agtgggtact tacgccaaag aactgcgcgg
    32221 tgcgacgaaa gagaaagaga gcctgccgca gatgctgcgt ggtttaagca agctgcgtaa
    32281 tctcggtcag gggtacgtca acttcggtga accaatgccg ttgatgacct accttaacca
    32341 gcacgtacca gactggcgtg aatctatcga tcccatcgaa gcggtgcgtc cggcctggtt
    32401 aacgccgacg gtcaataata ttgctgccga tctgatggta cgcattaaca acgcaggcgc
    32461 ggcaaacgcc atgaacctgt gctgtactgc gcttctggcg tcacgtcagc gctcactcac
    32521 ccgcgagcag ttaaccgagc aactcaactg ctacctggat ctgatgcgca acgtacctta
    32581 ctccacggac tctaccgttc cttcagccag cgccagcgag cttatcgatc acgcgctgca
    32641 aatgaacaag tttgaagttg agaaagacac tatcggcgac atcatcattc tgccgcgcga
    32701 gcaagcggtg ctgatgacct actatcgcaa caacattgca catatgttgg tgctgccttc
    32761 gctgatggcg gcaattgtca cccagcatcg ccacatctcc cgcgacgtat tgatggagca
    32821 cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc ctgcgctggg atcgcgacga
    32881 gttaccggac gttattgatg cgctggcaaa tgagatgcaa cgtcaggggc tgattaccct
    32941 gcaagatgat gagttgcata tcaacccggc gcattctcgc acgctacagc tgctggccgc
    33001 aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc tggctgttga gtgccaaccc
    33061 gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc gtcgcgcaac gtctctccgt
    33121 gctgcacggt atcaacgcgc cggagttctt cgacaaggcg gtgttcagtt ctctggtgct
    33181 gacactgcgt gatgaagggt atatcagcga tagcggcgac gccgaaccgg cagaaacgat
    33241 gaaggtgtat cagttgctgg cggagttgat tacatcagac gtgcgtttga cgattgagag
    33301 tgcgacgcag ggcgaagggt aatcagtgag aattgccgga tgcgacgcaa acgccttatt
    33361 cggcctacca tgatctgcaa attcaataaa ttgcgatatg ttgtgtaggc ctgagaagcg
    33421 cagcgcatca ggcaacccag aagaaagccg gatgatcatc cggctttttt acatcatcag
    33481 aaatgccagt aactcattgc cagccctaaa aacagtacca gaccaacata gttattattc
    33541 ataaatgctt taaagcaggc ttcacgctcg cggttggcaa tcagtttttg ttgataaaca
    33601 aacagcgcgc cagctaccag aattgaccag taatatcccc agcctaagcc atttaactca
    33661 ccgatgatcg ccatcagcgc tagtacgcca atctgcaaaa taccaataat caatttatcg
    33721 tattggccga acaggattgc ggtggattta atgccaatct tcacatcatc atcgcggtca
    33781 accatcgcat actgtgtgtc gtaagccacc gcccagagaa tattggcgag gaacattaac
    33841 cagcaactca atggcaccga ctcactcaca gcggcaaaag ccattggaat cgaccagcca
    33901 aacgccgcgc ccagcaccac ttgcggtaga tgggtatacc gcttcataaa cgggtatacc
    33961 cacgccagcg ccagcgcggc aatcgacaac agaatagtca tcgtattcag cgtcagcacc
    34021 agtaaaaatg aaatcagtac cagcactaca aacagcgcgc gcgcctcttt ctccgttacc
    34081 gcgccgctgg gaagtggtcg gttcgccgtg cgcttaacat gaccatcaaa cttgcggtca
    34141 gcataatcat tcaccacgca accagcagcg cgcatcagcc agacgcccgc gacaaacacc
    34201 gccaggatcc agagctgggg aacgcccggt gtcgccaccc acaacgccca taatgttggc
    34261 cagagcagca gtaacgcgcc aattggctta tccgtacgca ttaagcgatg aaacgccagc
    34321 agcttattct gcgtcagact ccactccata tttttttcct cttagtacaa cggtgacgcc
    34381 ggtaaaaaca gttctgttag caacagcggt ttaccgctta atcgcaggcg ggaacgtcgc
    34441 ccccacagtc cggcatcacg acctatctca ataaagtccc gggttaatgt cgatgatgtg
    34501 aacagatagc gtcctaacgg cgttttacct aatttttgta acgccagctc cggcccgctt
    34561 aacgttgaca caggaacgac ggtacgaccg gcaagccacg gttcaccatc ggcacataac
    34621 aaaatttcac gtaaccagta acgagactct ttcggcagca gtggcagttc ttcggggatt
    34681 tcattctgct cgacaaaccc ttcgcggatc atcgtcacgc tcaccgtttt tccctgctgt
    34741 tcaaaacgtt ttgtcatgga atcttccagc aacagccagt cgagcagttg cggctccagg
    34801 gcagggatct ctgtaaaata gcgcagcgca cgcagttgcg ttaacgcggg gtgtgacatg
    34861 ccgaactctc cgttacataa cgtaaaggca ttgtatcgca gaaaaatatt tacgggagat
    34921 gtaaagcaaa ttaagtgaac taatgcgcaa caaaaacgca acaatagtac aggaggagaa
    34981 aaaaaggtgc gccaggagac gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta
    35041 ccccttgcct tttacactgc tgataaaggt ggaacgggca gatgtcgacc ccaggcgttc
    35101 agcttcatca agcagtttta acgccttatc aacatcacct ttcgcgacag cgtttttgat
    35161 cgcggtatta aaataacttt ccgtgtcgtt gagcatcggc tcgcttttct tcaccggtgc
    35221 cggagcgggt gcagccacag ctggtgccgc cgtgttgcct accgtaaccg gagctggagc
    35281 ggaagaacca aatagtggtc ctaccaacac gctggagctg gagttcgttt tcactttcag
    35341 tttcagtaag ccatcggtgg tatgacgagc aaccggatcg ggaatatccg ggatcgagtt
    35401 accgacgcct ttggcatagg ctttagccgg gtcgagcagt tgagtcgtct gctggagatc
    35461 tttttccgtg gtaaagacca gaacataaag tttttgctgc cccaacgccg gtgtcaggcg
    35521 cataacgcct tccagccgat ctgcactcat cacgccgggt tcctggtagg tgaaataact
    35581 gctggggaag aaggctgatg gggtcatgtt ctgatcaaga atcagcacat tcggcgcaaa
    35641 tacgctggtt tgtttgttca cttcgctggt cagcgtcagt gtcagttcac caatgtttgc
    35701 cgggacgctg tacgcagcaa ccggaccact gatgccaggg acattcagtt gttggccgcc
    35761 ggtcgacagt tgagtagtct gggttttaga ttgatcgacc ggtgtccagg tgagttgttg
    35821 cagcgcggca gatggaatgg ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc
    35881 ggcaaggctg attccaggtg cgcttgccag caaccctgct gataaacaga gagcgatgag
    35941 acttttattc attttcattg ttttcacctc aaaatctgga gctcagcggt agccaggcaa
    36001 tagcgcgcta aacccgataa tcagaggggc tttcgcccct taaagctgtt tttatgccgg
    36061 atgcggcgta aacgccttat ccggcctact ttcggcagca gttgtaggcc tgataagacg
    36121 caacagcgtc gcatcaggca ttggttgccg gatgcgacgc aagcacctta tccggcccag
    36181 gttttgctat taccaccaga tttccatctg ggcaccgaag gtccactcgt cgctgtcgcc
    36241 acgaccgaag ctaccgccgt tgaaatcagc cgacacagcc ttgccgtagt atgggttggt
    36301 tgaagagcta ccggtgtagt cataacccca tttctcatcc cacttggcgt aggttgcgaa
    36361 gacacgaata gccgggcgtg accagatgct gtcgccagcc tgccattgtt gtgcaagggt
    36421 aattttgtac tgattgttct tgtcgccggt gcgctgggat tcgacgttgt cgtagccgat
    36481 ttccatcacg gtgctcatga ttggcgtcca cttgtacatc gggcgaatac cgacggtcca
    36541 ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg tacataccca cgtacatcat
    36601 gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg atacgcagca tgtgaccgtt
    36661 gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg acgccagaac cctgcgacag
    36721 acctttaccc tgcgaggtca tcgagtcagt agcgtactga acaacaaact tgttaaagcc
    36781 cttcaggaca ctctgagtat gttcagcagt gaataaccag ccgtctttcg atgcgccatc
    36841 aaccagacga tagttatcac gcaggttggc acgaccgtag tcgacaccca gttctaatgt
    36901 gccgcccggg ttgatttcca tctgcgctaa acgcacatcg aaaacgtcgt tcgcggtttc
    36961 gttggtatag tcataaatat tgttgctggc gaaagaggaa gaaccacccg cttcagagga
    37021 gcgggttgct gccagagaga gtttaccgaa gccaacatcg atgttttcca gaccggcacc
    37081 aggaccagaa atatcccagt agtagaagtc gatcatatga acgtcatgac gttggtagaa
    37141 gcgcttacct gcccagatgg tggagcctgg cagccattcg atcaggtttt taccctgcac
    37201 gtttgcttca cggaaggccg gatcggtagc ttcccagtca ttctgttgcg cgacggaata
    37261 ggccacgtta gtgtcgaaat agaagctctt atcgccctct ttccacactt cctgacccaa
    37321 ttttaattca gcataagttt cacattcgtt gccaagacgg tatttacttt gagcaccggt
    37381 agtctggaaa cactgttgtt cgccgccgct acctgtccag ccaataccgg aacgtgcata
    37441 gccgtggaaa tcaacagcca ttgcctgagc agacattacg cccgctgcga cggcaaccgc
    37501 cagaggaagt ttgcgcagag taatcatcat tctatctcct gagtcattgc ttttcttttt
    37561 tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg cttaaacgcc cggctcctta
    37621 tgcagtcgac gacatgcagt gccatcctca cggaacagat ggcaacgctc tggcggcagg
    37681 ccgatagcga atgtggcacc ttcttctacc aacaccacgt cgttctggcg gtacaccagg
    37741 ttttgacgaa tggaagggat ctggatatgg atttgggttt cgttgccgag ttgctcgacg
    37801 acctgaactt caccctcaag gatgacgtca gcgatatcac tcggcagtag atgttccggg
    37861 cgaataccca gcgacatatt ggctccaacc tggacatcac ggctttcaac cggcagccag
    37921 acttgctgac gatttggcat cggcagctcc acctgcactt gatcgattgc ggtggcggtc
    37981 acttttaccg gcaggaagtt catctttggc gaaccgataa atccggcgac aaaacggtct
    38041 gccggatagt ggtacagctc cagcggtttc ccaacctgcg ccacgcgacc ggcgtccagt
    38101 accacgattt tgtcggccag cgtcatcgct tcgacctgat cgtgggtgac gtaaatcatt
    38161 gtgcggccca ggcgtttatg cagacgggag atttcgatac gcatttgcac acgcagtgca
    38221 gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc ttggctcggc caccagcgta
    38281 cggccaatcg ccacacgctg acgctgacca ccggagagcg ctttcggttt gcgatccagc
    38341 aaatgcgcca gttgcagcac ttccgctacc tggttgacgc gttggttaat cacctctttt
    38401 tttgcgcctg ccagtttcag gccaaatgac atgttttctg ctactgacag gtggggatag
    38461 agcgcgtaag actgaaacac cataccaacg ccgcgttctg ctggcggagt gtcattcatc
    38521 cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct caagcccggc aatcatgcgc
    38581 agtaaagtcg atttaccgca gccagacggt ccgacaaaca ccacgaattc accttcatgg
    38641 atatcgagat tgatatcttt cgataccacg acctcgcccc aggctttcgt tacattttgc
    38701 agctgtacgc tcgccatgcc cttctccctt tgtaacaacc tgtcatcgac agcaacattc
    38761 atgatgggct gactatgcgt catcaggaga tggcttaaat cctccacccc ctggcttttt
    38821 tatgggggag gaggcgggag gatgagaacg cggcttctgt gaactaaacc gaggtcatgt
    38881 aaggaatttc gtgatgttgc ttgcaaaaat catggcgatt ttatgtgcgc atctccacat
    38941 taccgccaat tctgtaacag agatcacaca aagcaacgat ggggcgtagg ggcaaggagg
    39001 atggaaagag gttgccgtat aaagaaacta gagtccgttt aggtgttttc acgagcactt
    39061 caccaacaag gaccatagat tatgaaaata aaaacaggtg cacgcatcct cgcattatcc
    39121 gcattaacga cgatgatgtt ttccgcctcg gctctcgcca aaatcgaaga aggtaaactg
    39181 gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg taagaaattc
    39241 gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga agagaaattc
    39301 cctcaggttg cggcaactgg cgatggccct gacattatct tctgggcgca cgaccgcttt
    39361 ggtggttacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc gttccaggac
    39421 aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat tgcttacccg
    39481 atcgctgtcg aagcgttatc actgatttat aacaaagatc tgctaccgaa cccgccaaaa
    39541 acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg
    39601 atgttcaacc tgcaagaacc atacttcacc tggccgctaa ttgcggctga cgggggttat
    39661 gcgttcaagt atgaaaacgg caagtacgac attaaagacg taggcgtgga taacgctggc
    39721 gcgaaagcgg gtctgacctt cctggttgat ctgattaaaa acaaacacat gaatgcagac
    39781 accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat gactatcaac
    39841 ggcccgtggg catggtccaa catcgacacc agtaaagtga attatggtgt aacggtactg
    39901 ccaaccttca aaggtcaacc atctaaaccg ttcgttggcg tgctgagcgc aggtattaac
    39961 gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg aaaactatct gctgactgac
    40021 gaaggtctgg aagcggtcaa taaagacaaa ccgctgggtg cagtagcgct gaagtcctat
    40081 caggatgaac ttgcgaaaga tccacgtatt gccgccacca tggataacgc tcagaaaggt
    40141 gaaattatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg tactgcggtg
    40201 atcaacgccg ccagcggtcg tcagactgtg gatgctgcgc tgaaagatgc acagactcgt
    40261 atcaccaagt aatgctgtga aatgccggat gcggcgtgaa cgccttatcc ggcctacgaa
    40321 attgaaacgt atgtaggcct gataagacgc gtcagcgtcg catcaggcag ttgttgtcgg
    40381 ataaggtatg aaagccttat ccgtcctgga atgaggaaga accccatgga tgtcattaaa
    40441 aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag tgttaggtct gctcggcctg
    40501 ctggtgggtt accttgttgt tttaatgtac gcacaagggg aatacctgtt cgccattacc
    40561 acgctgatat tgagctcagc ggggctgtat attttcgcca atcgtaaagc ctacgcctgg
    40621 cgctatgttt acccgggaat ggctggaatg ggattattcg tcctcttccc tctggtctgc
    40681 accatcgcca ttgccttcac caactacagc agcactaacc agctgacttt tgaacgtgcg
    40741 caggaagtgt tgctagatcg ctcctggcaa gcaggcaaaa tctataattt tggtctttat
    40801 ccggcgggcg atgagtggca actggcgctc agcgacggcg aaactggcaa aaattacctc
    40861 tccgacgctt tcaaatttgg cggcgagcaa aaactgcaat tgaaagaaag cgccacgcag
    40921 cctgaaggcg agcgcgcgaa tctgcgcgtg attacccaga atcgtcaggc actgagtgac
    40981 attaccgcca ttctgccgga tggcaacaaa gtgatgatga gctccctgcg ccagttttct
    41041 ggcacgcaac cgctctacac actcgacggt gacggcacgt tgacgaataa tcagagcggc
    41101 gtgaaatatc gtccgaataa ccaaattggc ttttaccagt ccattaccgc cgatggcaac
    41161 tggggggatg aaaagctaag ccccggttac accgtgacca ccggctggaa aaactttacc
    41221 cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg ccattttcgt ctggaccgtg
    41281 gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg gcatggttct ggcgtgtctg
    41341 gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg tcctgcttat tctgccctac
    41401 gcggtgccat cgttcatttc aatcttgatt ttcaaagggt tgtttaacca gagtttcggt
    41461 gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc ccgcctggtt cagcgatccg
    41521 accaccgccc gcacgatgct gattatcgtc aacacctggc tgggttatcc gtacatgatg
    41581 atcctctgca tgggcttgct gaaagcgatt ccggacgatt tgtatgaagc ctcagcaatg
    41641 gatggtgcag gtccattcca gaacttcttt aagattacgc tgccgctgct gattaaaccg
    41701 ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta acaacttcgt gctgattcaa
    41761 ctgttaacca acggcggccc ggatcgtctt ggcacgacca cgccagccgg ttataccgac
    41821 ctgcttgtta actacaccta ccgtatcgct tttgaaggcg gcgggggtca ggacttcggt
    41881 ctggcagcag caattgccac gctgatcttc ctgctggtgg gcgcgctggc gatagtgaac
    41941 ctgaaagcca cgcgaatgaa gtttgattaa gggagataac aaaaatggca atggtccaac
    42001 cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct actgctactt tttatcgcgg
    42061 cgattatgtt cccgctgctg atggtcgtcg ccatctcgct gcgtcagggg aacttcgcga
    42121 ccggtagtct gatcccggag caaatctcct gggatcactg gaaactggcg ttaggtttta
    42181 gtgtggaaca ggctgatggt cgcattacgc caccgccatt cccggtactg ctgtggctgt
    42241 ggaactcggt aaaggtcgcc gggatttccg cgattggcat tgtggcgctc tccaccacct
    42301 gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc gacgctgctg aaaggaatgc
    42361 tgattttcca gatgttcccg gcagtacttt cactggtcgc gttgtatgcg ttgtttgatc
    42421 gtctgggtga gtacattcca ttcattggcc tgaatactca cggcggcgta attttcgcgt
    42481 atctgggcgg gattgcgctg catgtctgga ccatcaaagg ttatttcgaa accatcgaca
    42541 gttcgctgga agaagcggcg gcgctggatg gtgcgacacc gtggcaggcc ttccgccttg
    42601 tcctgttgcc gctgtcagta ccgattctgg cggttgtgtt catcctgtcg tttatcgctg
    42661 ccattactga agttccggtc gcgtcgctat tactgcgtga cgtaaacagc tacaccctgg
    42721 ccgtggggat gcagcaatac ctcaacccgc aaaactacct gtggggtgac tttgccgccg
    42781 ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt gctggctcaa cgctggctgg
    42841 tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg ttgttctgcc aatgttatgc
    42901 cgctgcaccc tcaacttacg ttatcccaac ttgtgactgt tattcggcgc tccacggagc
    42961 gccttttttt actcccgctt cagccgtttc gaattacaca gccacagggt gatcaccagt
    43021 aacaggatcg ctgccgagta gatcaacaca tccagtggcg atttatgatc gacgatgatc
    43081 aagcgcacaa tggcggtgat cccaatatag acaaagtaac gtagcgggaa gtgaaaaccg
    43141 gactgaaagt acttcacaat cagcgcgata aattcgaaat agagaaagta aaccaccagc
    43201 ccttctacca gctcatattt actggtttgt tctggcgcga acagcacatc agccagatgc
    43261 accgtttctt tgccgaggaa gacaaccaaa atcaggccga ggcacaacag gccaagattg
    43321 agtacggtct gcaaaatggt ggatataaac tccacgcgcg gacgagagag tgacgtcata
    43381 gcagtacctc cttcacagtg gcgagcttcc tgtataacgc aaaaatgtga cggagatcta
    43441 tattttttgt gtatgttttg ttcagctcaa ttaagtgggc atctcccatc aatgtctcat
    43501 ctggcaaaac acacagccaa aaaaatcacc cattcaggtg attcccagcc accagcaatg
    43561 cgcgaaaaat cgacaatgtt cctctggaac aaatgaaacg taacctgata aaggtggtaa
    43621 aaatgaagcc ttattttgct gctttgatgt tatcagtctc tgttcttcct gcttatgctg
    43681 gtccgctcgg tactgctgat aaagccgacc taccgcaaag caatgtctcc agtccaatga
    43741 tggcccagtc gctcaggcaa ccagacctgc aaccgatatc gacggacagg aagacagaat
    43801 gttttcgcct ttacacacca gatcgcaaac cgggggtgaa ctgcgttcct gatggctcga
    43861 caggccatta agaatgaagg tgccagtgag tgcgaaaact ggcacctttc ccatgactta
    43921 gcggaaattg acgctacggt cgctggtcat atcatacaac tggaacttac gcccaagttg
    43981 ctgaccaccg tcacgcgtca gcggcgtcca gccaatcgcc gcacggctgc gtgtcgggcc
    44041 agacgagaag agatccagcg gtacagagac atacacgcct ttggtgaagt ccccttcgcc
    44101 gtactcctct ttcgaaacat tcgtgatcgt ggcatagccc cccactacca cgccgctatc
    44161 aaagcgtttg gcgatctcca gcgtgccgcc tttatcccca gccagatact gcccgacgct
    44221 cgctttcact aacacgtcct gggcgaaaga tggcgtccag tacgcggtca gatgtccggt
    44281 tttcacgctg taatcggtga atttcatcat atcttttgcg ctacgccagt cgcgctgttt
    44341 aacgtagttg gcatccagac caaacgccca gttgctatcc agcgggcgat acaacacttc
    44401 tgcccccacg ccgccaaaca tggtttcgag ataacctccg tagacctgac cgtagaagcc
    44461 gttgcccaga tgctggaagt agttggcttg caggttattc acatagacat cattctgcac
    44521 atactcgcgc acatgggtac gcacgcgcgg taagtgcgag tcctgcggtg gattggtgta
    44581 gttaaacttg tcgtagttgt tggcaaggtt agcaaacagg ctgccggtgg tcagcagatg
    44641 gtccgtcagc cacaaatccg ccgttcccat cacgcccagc tgatacatgt aaaagttttc
    44701 cgggccaccg accgactggt tcagcaccgg atcgatatgg aaatcaaagc gtgatttgtc
    44761 gatataccag ccctgctcgg tggacttcgg aaccactggc tcgacgcgtt tttgcgccag
    44821 cgtcgtttcg tggcccaacg gctctccgcc gagatgattt ttcaggctgg cgacatcggt
    44881 ttccgtcgtc acctgcggca tgttaaggcg attttccgta atgcggatcg ttttgatccc
    44941 atccggcaga tcgttcatca cgatccgatt agcacggatg atcccttcgc gcgaatcacg
    45001 atatttcacc tgctcgccgg taacatacag cgtatcgcct ttcgcctgga tctgcggatc
    45061 ggccagtccg gcgttgtatt tcaacagcgt taactgattc gccaccaccg agtgctgcaa
    45121 aatggcatcc tgcggttgcg gctggtattt cggtcgggcg ttatcgttgt aggacgggcg
    45181 cagatcgtta aagttggtgc gcagcgtgac gccaaacata aaggtgttgc cacgttcata
    45241 gctaaggtta acgtcggccc aatcggtaac gcgataaatc gccccgacgt taaacttact
    45301 tttttgctcc agcttcccgg caaaatcctg ctgataatta ttaccttcat actccagttt
    45361 caggcgcagc ggttgccagg gcgtctggta ttccacgccg ccaaacagtg aggccgggcc
    45421 gtggaacatc tgactgccgt cgatagatcc cgcttgtttg tagctgttat cgcgatagca
    45481 atatttatca ctggctgaac agagcggatt tttcacatta ccgctggtgc ccagatatcc
    45541 ccatcccagg ccgagcgtaa aatcgaacgg cccccaggct ttgctggcaa caagatattc
    45601 cgcatcaaac agccccgtac cgccgatatc ccgcgcacca accgccactt gcggcagcca
    45661 ataactctct tcccacaaac gcagtttgag atcgaaggct ttatctttat acgtttgatc
    45721 gccagagaac gcttcgacgc tgctgtactg ccgggtgcgc acgtcggtgt agcgcagcgt
    45781 tgtttccagc cacgggaaga gttgcactga agctgagtaa taacggtact gatcgttatc
    45841 gcgatagttc aggctcagct ccccttcccg cgccatgcgc gcggtgggcg tttgtaataa
    45901 tcctacgcca ccgaaatccg attgcgacgg accaatgggc gcaggatatg tttcgccgta
    45961 gcaagctgtg ctaatgccca gcgccagtaa gctaagcaga tgtctttttt tcattattgg
    46021 ggtatccgct gcgtcagagt ctgaagaatg tcggcgttaa gggcatcagg tgtctcactc
    46081 cagacggagt ccgcgaggcc aacataaata atgctgccgg gcatcggctc tacgtgacgc
    46141 ttgttccaat aagccaccgg cgctttttgc gtgcgtccgt ccgggtaaac cacccacgcg
    46201 tagctgcgat ccgcaccgct gagcaggctc tggtcagaga gatagctcgc cacgtcgcga
    46261 cctggagtga atgactgctt gccaggacga ctgataagcc cgaacaacgt gaccgtggac
    46321 ggcggcgttc cgacccacag cgtgtagttg ccctgcaacg gcgggttacc gcgttcggca
    46381 acgcggacga tatcaggatc aagattgatt ttttgcctgc ccgtcacttt caacgcctga
    46441 atttgctggc gtaaggcgtt aatcgcggcg gcatcgtcgg cgctggaatc tgcagccagt
    46501 tctgccagcc gtgtcagcaa cgcctgctgc tgacgcaatg ctgccgccgt tgccagctct
    46561 tcgctaatca ccgcaccggg ccaccaactg tttgccagcc gtggttggcc taccagatcg
    46621 attaaatgct ctgcacccgt cagcgttttg gcctcactgc tgccattgct gaataccttg
    46681 acggttcccg ccgcgaagac tgatgacgcc cccacactca aaatcaatgc gacaatagtt
    46741 tgtttaatca ttgttttgcc gccttgatga gggtggtttt caccgggaaa taatccgcgc
    46801 cgagatactg ttccgactgg cgaatttgcc cttcgctgtc gatccagtag cggttatgcc
    46861 agcgcgcctg gtcggtggag acttcttcgt ccagcacgcg aaccggggtt tcatcgctgc
    46921 cgactttgac ggtatcggtg ccatcccatt tgaagactga gcgtgcggtg gcgtagcgta
    46981 cctgctggta ctcggtccag cccatcgtgc gcgtccatgt tgcaccatca acaatttgtg
    47041 cgggtttaat cagcgggtca gtggcaaggt tattcacttc aatcaggttg tcgccaccaa
    47101 gcaaagtctt caccagacga ccatgttgtg tgacgagagt ggcctgatcc tgagtgaccc
    47161 atttttgttg tccgtcttca gcgaaggcca gcaccacaaa taactgcggc ccgccattaa
    47221 gctgcatgta ctggctggcg tagggcatat tttgaatatc atcgtccgtc agctgtacgc
    47281 ctggcgtgcc gaacagactg tcccacagtg aattgcccag ctctttagtc gtggctgaac
    47341 aggcctgtaa tagcaggcag ataagaatga gtgcaggtcg cttcacgact cttctccaga
    47401 ggggcagaat aaccacaccg gagtgtggtt atggttaatc cccttcatac ttcaagctac
    47461 agatgcgatg actcgaatta ttttggggat acatacttta ttactgggta ctggtggtcg
    47521 tggttgtggt ggtcccggta ttagaaccat caccgccacc ggttgccgcg agcgcgacac
    47581 ccaccgccga gcttacggtg ctgacgctgg tcgcggagga actccccgcc gaaaccgacg
    47641 ttgctgccga tcctgccgct tcgcccacct gtacaggtgc agcccacgca gaagtcgccg
    47701 caagcgcaga tatggcaaaa atgccataca gaactttttt cataacaatt tcccttcatt
    47761 gaatgaatgg agatttaccc aaaacatttc gggcgggatc gagtatacac aactaaagca
    47821 tgcgaattac ggcgcgggga attagtgatg gagttttagg acaattggaa tgaa
//
LOCUS       DAZKHK010000018        47607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKHK010000018 DAZKHK010000000
VERSION     DAZKHK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.018"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOS5_001092"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHOS5_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP9775946.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALQQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFSVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPEVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOS5_001093"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHOS5_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP9775947.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMSWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3103..3351)
                     /gene="hybG"
                     /locus_tag="ACHOS5_001094"
     CDS             complement(3103..3351)
                     /gene="hybG"
                     /locus_tag="ACHOS5_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP9775948.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3364..3705)
                     /gene="hypA"
                     /locus_tag="ACHOS5_001095"
     CDS             complement(3364..3705)
                     /gene="hypA"
                     /locus_tag="ACHOS5_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9775949.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3698..4186)
                     /gene="hybE"
                     /locus_tag="ACHOS5_001096"
     CDS             complement(3698..4186)
                     /gene="hybE"
                     /locus_tag="ACHOS5_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP9775950.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4179..4673)
                     /gene="hybD"
                     /locus_tag="ACHOS5_001097"
     CDS             complement(4179..4673)
                     /gene="hybD"
                     /locus_tag="ACHOS5_001097"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP9775951.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4673..6376)
                     /gene="hybC"
                     /locus_tag="ACHOS5_001098"
     CDS             complement(4673..6376)
                     /gene="hybC"
                     /locus_tag="ACHOS5_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP9775952.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6373..7551)
                     /gene="hybB"
                     /locus_tag="ACHOS5_001099"
     CDS             complement(6373..7551)
                     /gene="hybB"
                     /locus_tag="ACHOS5_001099"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP9775953.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7541..8527)
                     /gene="hybA"
                     /locus_tag="ACHOS5_001100"
     CDS             complement(7541..8527)
                     /gene="hybA"
                     /locus_tag="ACHOS5_001100"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP9775954.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8530..9648)
                     /gene="hybO"
                     /locus_tag="ACHOS5_001101"
     CDS             complement(8530..9648)
                     /gene="hybO"
                     /locus_tag="ACHOS5_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP9775955.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9837..10124)
                     /gene="yghW"
                     /locus_tag="ACHOS5_001102"
     CDS             complement(9837..10124)
                     /gene="yghW"
                     /locus_tag="ACHOS5_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP9775956.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10243..11130)
                     /gene="yghX"
                     /locus_tag="ACHOS5_001103"
     CDS             complement(10243..11130)
                     /gene="yghX"
                     /locus_tag="ACHOS5_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP9775957.1"
                     /translation="MPRLTAKAFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11288..12328
                     /gene="gpr"
                     /locus_tag="ACHOS5_001104"
     CDS             11288..12328
                     /gene="gpr"
                     /locus_tag="ACHOS5_001104"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP9775958.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12368..12862)
                     /gene="yqhA"
                     /locus_tag="ACHOS5_001105"
     CDS             complement(12368..12862)
                     /gene="yqhA"
                     /locus_tag="ACHOS5_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP9775959.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13053..13937
                     /gene="yghA"
                     /locus_tag="ACHOS5_001106"
     CDS             13053..13937
                     /gene="yghA"
                     /locus_tag="ACHOS5_001106"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9775960.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13976..14401)
                     /gene="exbD"
                     /locus_tag="ACHOS5_001107"
     CDS             complement(13976..14401)
                     /gene="exbD"
                     /locus_tag="ACHOS5_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP9775961.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14408..15142)
                     /gene="exbB"
                     /locus_tag="ACHOS5_001108"
     CDS             complement(14408..15142)
                     /gene="exbB"
                     /locus_tag="ACHOS5_001108"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9775962.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15394..16581
                     /gene="metC"
                     /locus_tag="ACHOS5_001109"
     CDS             15394..16581
                     /gene="metC"
                     /locus_tag="ACHOS5_001109"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP9775963.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16721..17380
                     /gene="yghB"
                     /locus_tag="ACHOS5_001110"
     CDS             16721..17380
                     /gene="yghB"
                     /locus_tag="ACHOS5_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP9775964.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17420..18319)
                     /gene="yqhC"
                     /locus_tag="ACHOS5_001111"
     CDS             complement(17420..18319)
                     /gene="yqhC"
                     /locus_tag="ACHOS5_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP9775965.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18513..19676
                     /gene="yqhD"
                     /locus_tag="ACHOS5_001112"
     CDS             18513..19676
                     /gene="yqhD"
                     /locus_tag="ACHOS5_001112"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9775966.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19781..20608
                     /gene="dkgA"
                     /locus_tag="ACHOS5_001113"
     CDS             19781..20608
                     /gene="dkgA"
                     /locus_tag="ACHOS5_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP9775967.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20808..21734
                     /gene="yqhG"
                     /locus_tag="ACHOS5_001114"
     CDS             20808..21734
                     /gene="yqhG"
                     /locus_tag="ACHOS5_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP9775968.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     PIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21785..22042
                     /gene="yqhH"
                     /locus_tag="ACHOS5_001115"
     CDS             21785..22042
                     /gene="yqhH"
                     /locus_tag="ACHOS5_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP9775969.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22084..24303)
                     /gene="ygiQ"
                     /locus_tag="ACHOS5_001116"
     CDS             complement(22084..24303)
                     /gene="ygiQ"
                     /locus_tag="ACHOS5_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP9775970.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            24555..25304
                     /locus_tag="ACHOS5_001117"
     CDS             24555..25304
                     /locus_tag="ACHOS5_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9775971.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            25627..27099
                     /locus_tag="ACHOS5_001118"
     CDS             25627..27099
                     /locus_tag="ACHOS5_001118"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9775972.1"
                     /translation="MSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTLRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIKHDLQNPGAPLS
                     AIGVIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNTVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVSGRPEWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDVVEKSQSVADPMNDQFQTLIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            27096..28112
                     /locus_tag="ACHOS5_001119"
     CDS             27096..28112
                     /locus_tag="ACHOS5_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9775973.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVGNLDINAFDPTQKDFIA
                     ALREAFGGELACVVLDATGNKASMSQDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQEFDFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            28123..29133
                     /locus_tag="ACHOS5_001120"
     CDS             28123..29133
                     /locus_tag="ACHOS5_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFP9775974.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            29206..30189
                     /locus_tag="ACHOS5_001121"
     CDS             29206..30189
                     /locus_tag="ACHOS5_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9775975.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            30231..30713
                     /locus_tag="ACHOS5_001122"
     CDS             30231..30713
                     /locus_tag="ACHOS5_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9775976.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNSH
                     "
     gene            30724..32028
                     /locus_tag="ACHOS5_001123"
     CDS             30724..32028
                     /locus_tag="ACHOS5_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9775977.1"
                     /translation="MEFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(32099..33511)
                     /gene="ftsP"
                     /locus_tag="ACHOS5_001124"
     CDS             complement(32099..33511)
                     /gene="ftsP"
                     /locus_tag="ACHOS5_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP9775978.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(33586..34323)
                     /gene="plsC"
                     /locus_tag="ACHOS5_001125"
                     /gene_synonym="parF"
     CDS             complement(33586..34323)
                     /gene="plsC"
                     /locus_tag="ACHOS5_001125"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP9775979.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(34558..36816)
                     /gene="parC"
                     /locus_tag="ACHOS5_001126"
     CDS             complement(34558..36816)
                     /gene="parC"
                     /locus_tag="ACHOS5_001126"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9775980.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(36954..38561)
                     /gene="ygiS"
                     /locus_tag="ACHOS5_001127"
     CDS             complement(36954..38561)
                     /gene="ygiS"
                     /locus_tag="ACHOS5_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9775981.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDDGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(38670..39152)
                     /gene="ygiV"
                     /locus_tag="ACHOS5_001128"
     CDS             complement(38670..39152)
                     /gene="ygiV"
                     /locus_tag="ACHOS5_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9775982.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(39205..39597)
                     /gene="ygiW"
                     /locus_tag="ACHOS5_001129"
     CDS             complement(39205..39597)
                     /gene="ygiW"
                     /locus_tag="ACHOS5_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP9775983.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            39749..40408
                     /gene="qseB"
                     /locus_tag="ACHOS5_001130"
     CDS             39749..40408
                     /gene="qseB"
                     /locus_tag="ACHOS5_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP9775984.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            40405..41754
                     /gene="qseC"
                     /locus_tag="ACHOS5_001131"
     CDS             40405..41754
                     /gene="qseC"
                     /locus_tag="ACHOS5_001131"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP9775985.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHTMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERYYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            41864..42445
                     /gene="mdaB"
                     /locus_tag="ACHOS5_001132"
     CDS             41864..42445
                     /gene="mdaB"
                     /locus_tag="ACHOS5_001132"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP9775986.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            42476..42790
                     /gene="ygiN"
                     /locus_tag="ACHOS5_001133"
     CDS             42476..42790
                     /gene="ygiN"
                     /locus_tag="ACHOS5_001133"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP9775987.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(42835..43722)
                     /locus_tag="ACHOS5_001134"
     CDS             complement(42835..43722)
                     /locus_tag="ACHOS5_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9775988.1"
                     /translation="MIQKDKARVDIFGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(43719..44666)
                     /locus_tag="ACHOS5_001135"
     CDS             complement(43719..44666)
                     /locus_tag="ACHOS5_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9775989.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(44677..45714)
                     /locus_tag="ACHOS5_001136"
     CDS             complement(44677..45714)
                     /locus_tag="ACHOS5_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9775990.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(45723..46706)
                     /locus_tag="ACHOS5_001137"
     CDS             complement(45723..46706)
                     /locus_tag="ACHOS5_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9775991.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(46703..47512)
                     /locus_tag="ACHOS5_001138"
     CDS             complement(46703..47512)
                     /locus_tag="ACHOS5_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9775992.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQFEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT        12083 a        12432 c        11732 g        11360 t
ORIGIN      
        1 ataatcagta cggggttgct acaacatgta ttactctttc accacaatta acggttcaat
       61 atcactctct tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgccaacg gtgaaagtac atacctgaat gtatttaccg tccacttccg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttaccg ctagtctgat cgagcacctc
      241 ttcatgatgg ctgacgaggt cgatattgct accacagcga ccggcaattg gtttcaccgc
      301 gtagccggtt ttcaccagtt catcattcac ggagaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 tagcggctca aagaccagca cttccgggcg cagcaatacg tcgataagac gcacttcatt
      481 ttgcggatga ccggtccgaa tcggcactgc ggcaaattca cggtcgctca cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacaca cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tctagcccgc gcaagatacg
      661 cgtttcaaag cccgcctgtt gcagcgcctg ctccataaac tgagcgtgat agttctcttc
      721 aatatctttg tcctgcatga tatggacaaa cggacgcgcg cgactgtgtt tccaggcacc
      781 agccagttcg ttaatcagcc cttccgcagg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcagaat cggcgttgta
      901 ttcgtagact ttcaggccac gttcatccat gcagaaatcc atacgaccgg tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg gaatgtcgaa
     1021 caacgccagc aggttgtcat ctttcagcac cttgtcggtg gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa tcagctcctg ctcggcactc tcggtaatgg tgtagtaatg
     1141 atacggatcc tgattgatca cctggccgtt ggcctgtaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccagacgcg ccccgctgat
     1261 tttcagcagt tcgcctgcaa tttccggctg tggcaagctg tattcggtat cttccgtctg
     1321 gatcatccag cccagaatag tggtgtcatc gaaggtgtct ttcagggtat agccgccgtt
     1381 ttcgaccacc atctccagct cgcgtgtcca ctgttgccct tgcggcaacg gggtatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcccccg ctaccggtgc
     1561 acgcggcgaa ccgttaggaa atgcctgcaa tggcaggatg ttgtcattga ccacttcacg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtgaaaa ccacaccgta
     1681 gttcagaaag agaaaacggc gagcaaattc aacgcattgc catttgtgac ccatatattc
     1741 atcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc ggtgggcaaa
     2221 catccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctac tggcactggg cgttactggt gcagagtacg acgcctggct gattcgcatt
     2341 ggcgatggcg atcaattctc cagcggcttt gtcgaagtga acccgaattc gaagatcccg
     2401 gcattgcgcg atcataccca taatccgccg atccgcgtgt ttgagtcagg ctcgattctg
     2461 ctttatctgg cggagaaatt tggctacttc ttgccgcagg atctggcaaa gcgtactgaa
     2521 acgatgagct ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc ggtgaaaatt gagtacgcca tcaaccgctt tactatggaa
     2641 gccaagcgcc ttctcgacgt gctggataag caactggcgc agcataagtt tgttgcggga
     2701 gacgaataca ccattgcgga tatggcgatt tggccgtggt ttggcaatgt ggtgttaggc
     2761 ggcgtatatg atgccgcaga gtttcttgat gcgggcagtt ataagcatgt acaacgctgg
     2821 gcgaaagaag tgggcgaacg tccggcagtg aagcgtgggc gtattgttaa ccgcatcaac
     2881 ggaccgctga acgagcagtt gcatgaacgc catgacgcca gtgatttcga gacgaatacg
     2941 gaagataagc gtcaggggta atggttagcg ttcgtcgcag caagccatcc agaccaatgt
     3001 aggccggata aggcgttcac gccgcatccg gcaatcaggc acaatgcctg atgcgacgct
     3061 gccgcgtctt atcaggccta cgaaagcatc cggctttaat catcacgcgc tggtaatgtc
     3121 gtaatccatt tggcgcagtg cgtctaatgt ggctttggct tcgtcttcat cgataatgct
     3181 catggcaaat ccgacgtgca ccagtaccca ctggcccagc agatcggcag ggttaccttc
     3241 acaaatcagg gcgatattca cgtcgcgctt gataccacat acttcaacct gcgcaagctg
     3301 gtgaatatct tcaccgacag ccagcacctg gcctggaacg ccaatacaca ttgttaactc
     3361 cggttattca acttcaatac ttttgacgat tagcgaatcg ccggtatcga cacgcaaccg
     3421 ctcgccatga cagatcggac actgcgcatc gtgctgatga atctccacca cctggctgca
     3481 atcccaacac caggcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgctac
     3541 cgttccgtgg cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag
     3601 tgcgccaatt tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg
     3661 ttggataatt tcaacggcgc tctggcaaag cgacaactca tgcattttcg ccactcctgc
     3721 ggccaaatag cagggcacga cgccctgcgt gtggtacatc cggattggtg actggcagcg
     3781 aaaggatcat tcgggcgcag tcatccgtca gccgttggcc ctcttcaatc gacatgctgt
     3841 gcgaaagcgg cgacatcagc gagcaggaga ggtattgcga aacaccgtcc agttcaccaa
     3901 cagtaaaggt catagtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca
     3961 gcggccagag ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca
     4021 cacaccccgt ccactgacct tcgaacagcg taaagtcaga aacatacacc ggcattgaag
     4081 gatgcagaaa agagagatca tgcatcgaac gccgggcaat ttcttcaaaa gctgcctgta
     4141 cttgcgcctt cggggaggtc tggaaacctg ctatctcttc agtcatgagt cgcctcccgt
     4201 gggatagctt ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca
     4261 atcatcgctt cgaccgtcgg cgtcaaaccg atgtgcggct ccagtgattc cgggatcacg
     4321 ccgaccaggg tcagcttttt cggaaactcg ccggtgaagc gcagagccga caatacgtcg
     4381 gccaggccaa gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc
     4441 cgcaggatca tcatcgttcc cggcgcgttc tttttcgaga caatggcatc cgcaataatc
     4501 agatgatcgc gatttgccat gtcgccaagc agctccattc ccgccgtgcc gccatcgagg
     4561 atctcaacat aatccggcag aatgtatcgt tgctctaacg cttcaacaat ccgcacaccg
     4621 atggcttcat cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa
     4681 ccttcactga aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc
     4741 acgggtcgaa ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcggcaaccg
     4801 gtgtacccac cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcgggccag
     4861 agttccaggt tgatggaaca actgcctggt agttgctgat gataccgtct ttgatcacca
     4921 tccagtgaga gagcataccg cgcggtgctt cgaggaagcc tacgcctttg aactcacccg
     4981 ttgccggaat gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca
     5041 gtgcactgta ttgattttgc aggatatcct gcaattcaca gcagtgaacg gtacgaccaa
     5101 taatacggcc cagcgtggag tgcagctgcg ccacttccag cgtgttgcca gtcagtttct
     5161 gataaatcgc aacgatttca ttcagtttgt tctgggtaga ctcgcgtcct gccgccagtt
     5221 tcaccagcat gttagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt
     5281 tcacccagga atatttaccg tcgtcagacc aaccatcata agccggaatg gtggtacctt
     5341 cccacggtgc ctgcggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatcc
     5401 ctttgatcag gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat
     5461 tctcaatgta gccgcccggg aacaggaagc tgccattttt actgtcggtc gggaattccg
     5521 gcacgctcag gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcag
     5581 cgataactgc agtatcaacc ttgtaaacct gctcaacaaa gtcgctcagc ttgtcgatga
     5641 aggacttgat gtacatcagg cgctcaaggt tcagcacacc cagaccgtcg aggttgatcg
     5701 ggttcgcgac accacctacc gccaggttct gaatatgcgg cgttttaccg cccagcagcg
     5761 ccacaacgcg gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa
     5821 tcaggttcac ttccggcggc agtttcattg ccgggtgacc ccagtagcca ttggcgaaaa
     5881 tacccaactg accgctggca accagatctt tgatcttgtt ctgaactttg gtgaactctt
     5941 ccgggctgtt cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggctggat
     6001 cagcttgcag tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa
     6061 caatatggtc atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa
     6121 ccggaacgtc gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg
     6181 tagtacatac gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt
     6241 tcacgatctc ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc
     6301 cattttcgat ttcgcaatcg atgcgtaaat gcccttcaat acgggttacc ggatcaatag
     6361 taattctctg gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg
     6421 aggaagtatc ggcagtagac gaatgagtac gatataagcg caaatctcaa tagccacaaa
     6481 accaatagaa atcaacagtt cttcccaagt cgggaagtag gcgtaaccgc cacccgggtt
     6541 gaatgccacc agcgaatagg tcagacgcca ggttgcacaa cctaacagtg cactcagtgc
     6601 tgacaggaac agcatgcggg aatcattacg caacttcgcc acacgcagaa cgaccagcgg
     6661 gaagagcatc agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc
     6721 taacgacagc ttgtcgcgat agataagctc gccaaagcgc agcacgatga aaatcgccag
     6781 caacacactg atggtattgg tcagcttaac gaacagactc ttttcatccg gaccgttgcc
     6841 acgcagaccc gcctgcacca acgaaccttc aaagatgaca atcgagaagc ccatgatgaa
     6901 cgccgtcagc agcgagaaca gcggcaacat ttcatagctt tgccacagcg gatgcacctt
     6961 gtagcccgcc gagatcatca gcgatcccat tgaagactgg tgcatagttg gcagcagcgc
     7021 accaagcgcg atgataaaga acatcacctt gttcagacgt ttgagtgaca ccttccagcc
     7081 cagacgttca aacaatgccg gagcaaactc cagtgccatc acaccgatgt agatggtcat
     7141 acagaccgcc gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta
     7201 cggcaggttc cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgaataacc
     7261 aaacagactc gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac
     7321 gtatactgcc cacgccagcg cccagccgcc acaggcaaag ccggtaccga tcagcaggtc
     7381 aaacgcgatc cacacgcccc acgggaagcc gccgttcagg tcggagacag agcccagacc
     7441 gaacaccaga cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat
     7501 gaccggttta ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc
     7561 ctccgtcgtg atggtcgttt ttagtgttgc gacgaaccag cacggttaag cccgccagca
     7621 cagccagtgg tagcatcatg cctttataca gggtgtgttg gacatgttcg gaacgcgcac
     7681 cagtagaaag atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta
     7741 ccagaacctg agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca
     7801 ccgtatgcag gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc
     7861 ccggcttcag cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccaaaaa
     7921 tcaccgcgcc cgccgggcac acttctacac agccaggcag accgccttta tcgagacgtt
     7981 ccacaccttt ctggttgcac aactcgcact tatgcagcgc accaaacggg ttgttgtagt
     8041 cgtacttcgg cacgttgtat gggcaggcga ccatgcagta acggcagccg gtgcacacat
     8101 ctttgtcgta atggacaatg ccggttttcg gatctttttt cagcgcagag accgggcaca
     8161 cagagacaca gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccat
     8221 tctcctcctg gtctttgttg acccctgtgc cgctggtcca cacctgaatg atgttattgg
     8281 tatacggcga cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag
     8341 ggaagttgat atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt
     8401 cgtacaacat ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga
     8461 cagacggcag cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc
     8521 tgttcacggt tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc
     8581 acgcaccgcc atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat
     8641 agcgcctgcc gagacgttgc cgccttcttt agcgttaacg tccggtttct gtgaacgcgg
     8701 agtctggttt tcgacgttgg caagctgatg gatgccttta tggaagccga taccttcttc
     8761 gttacagcca tagcacgggt gaccaatcgc taccggccac acgccgccaa catcgcagaa
     8821 ttgcagcgtt gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaggca
     8881 ccagccttcg cggtgacctt catcaccgaa ctctttggca aaacgaccag catcgaagtg
     8941 cgggcgacgt tcacagtgtt cgtgaatcag acgaccatag gcgaaagtcg gacggttttt
     9001 gtcatccagt ttcggcggtt tgccataagt gatgatgtgc gcaacggtcg ccaggaagtt
     9061 gtgcgggttc ggcgggcagc caggaatatt gataacggtt ttgcccggta gaacttcttg
     9121 caggctgact gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcagagca
     9181 ggaaccgata gcgataatgg ctgctgcgcc ttccgccgct ttgcggatgt gatccacaat
     9241 cggctcaccg gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac
     9301 cactaacaca tactgccctt tgtacttctc gagagcgtta tgtttgttct cttcgacctg
     9361 atgaccgaag gcggcggaaa gcacttcgtg atactccaga gagatagtct ccagcacgag
     9421 gttttctacc gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg
     9481 cgcgccaatc cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc
     9541 agcggctttg ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc
     9601 acgacggtta atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc
     9661 cgttgcgaag acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc
     9721 gccatttacc acacatttat tatggttatc gatatcatga tactgcggcg gggcactaaa
     9781 acgaagggaa gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa
     9841 ttttctaggc gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga
     9901 aagaaatcca ttaccatctc tttatccagt ccataacggc gcgtcagaat acccgcttcc
     9961 caggcgctaa gctggcgatc tccggtcttt tcgtacatcc ggtgtgagta gcctaataca
    10021 aaaccgcgtt tataatcggc acagaatttt gtcacattaa ccgcactgtc agcatgcgtt
    10081 gcttttaatc ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcgt
    10141 tatatattgt catatatagc gatattttag gcaaagaacg acatttgcgc acgctatttt
    10201 ttcatgcttg tttaagacgt aatcctgtaa gagataaacc catcaggaga gatatttatc
    10261 gaaccatttc agcgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt
    10321 ggaatcatta tggaatccgt gattaacccc cggatatata tacgcctcat agaccttatt
    10381 attggctttc aacgccgcct cgtaagcggg ccagccctcg ttgattcggc tatccagttc
    10441 agcgtagtgg agcagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg
    10501 cccataaaac ggcaccgcgc aggccagttc cggatacgcg actgctgccg cgttcgatac
    10561 gcctccgcca tagcaaaatc cgctaatacc cactttgcct gtcgcttgcg gatagcgttg
    10621 cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg
    10681 cagctcacga cctttatcat catttcccgg ataacctcca acggaactta agccgtcagg
    10741 tgccagggcg atataccccg ccttcgccac tcgccgcgtc acatcttcga tatacggatt
    10801 cagccctcga ttctcatgca ccactaccac ggctggcgtt ttgccgctca tttttgcggg
    10861 cttcaccaga taaccccgta cctcgccgtg accatttggc gagggatacg tgatgtactc
    10921 tgcaacaatt tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag
    10981 caaatcaaac agcgctaatg ccgtcatccc gcccaccgca tacttcgccg caaggttgag
    11041 gaactcacgt ttcgagattt tcccgtgagc gtaatagtcg tagtaatcca gcaactcttg
    11101 tgggaaagct ttggcagtta aacgcggcat cgttgcactc ctcaactggt gtttttttta
    11161 agcaaagcat aagcgcgtgt ttttgcccag tttttcgtca ctctgtgagc cagactacgg
    11221 gatacgcgca ggcgaatcgc taaactagaa acattgtttc gaaattgaac ggtggaaagg
    11281 agaggtcatg gtctggttag cgaatcccga acgttacggg cagatgcagt accgctattg
    11341 cggaaaaagt ggtttacgcc tacccgcgtt atcgctcggt ttatggcaca atttcggtca
    11401 cgttaacgcg ctggaatcac agcgtgcgat cctgcgtaaa gcgtttgatt taggcattac
    11461 gcactttgat ttagccaaca attacgggcc gcctccagga agcgcagaag agaactttgg
    11521 tcgcctgctg cgcgaggatt ttgccgctta tcgcgatgaa ctgattatct ctaccaaagc
    11581 cggatacgat atgtggcccg gcccatatgg ctctggcggc tcacgtaaat acctgctcgc
    11641 cagcctcgac caaagcctga agcgtatggg gcttgagtat gtcgatatct tttactctca
    11701 tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct gcgctggctc atgctgtaca
    11761 aagcggtaag gcgctgtatg tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat
    11821 ggtcgagttg ctgcgcgagt ggaaaattcc gctgttaatt catcaacctt cgtacaattt
    11881 actgaaccgc tgggtggata aaagcggcct gctggatacc ctgcaaaata acggcgtggg
    11941 ctgcattgcc tttactccac tggctcaggg attgctgacc ggaaaatatc tcaacggtat
    12001 tccgcaagat tcacggatgc atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat
    12061 gctcaccgaa gccaacctca acagcctgcg cttattgcat gaaatggcac agcagcgtgg
    12121 acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa gatgagcgag tgacgtcggt
    12181 attgattggt gccagccgcg cggagcaact tgaggagaac gtgcaggcgc tgaataatct
    12241 gacatttagc accgaggagc tggcgcagat tgatcagcat atcgccgatg gagagctgaa
    12301 tctgtggcag gcgtcttccg ataaatgacc tgttaataac gggcgcgaga accgcgcccg
    12361 aatcagatca gtgattatga cgagtcagtc ggtcaagata gcccatcaca aatgcagaga
    12421 gcacaaacgt cagatggata atgacgtacc acatcagttt gttatcaggg acatttttcg
    12481 catccataaa gacgcgcagt aagtgaatgg aagaaattgc cacaatcgac gctgctactt
    12541 tgtttttcag cgacgttgcg tccattttcc ccagccagtt cagcttctct ttgttctcag
    12601 agatatcgag ttgtgagacg aaattctcgt aaccggaaaa catcaccatc accagcaaac
    12661 cgccaaccag cgtcatatcc accagcgaca gcaacacgag gatcaaatct gattccgcca
    12721 tcgagaagat attcggcagt acgtgaataa tctcctggaa gaacttcagc gccagggcaa
    12781 ctaacgccag cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa gcatacattg
    12841 cattttcaag aaaacgttcc atacattcct gtcgtgtagt taaaacgggc aacagtatat
    12901 cgcaattaag taaacagcac gcaacagcaa atcaacatat acgttaaacc tttgtcgcat
    12961 aattctgagg gttagggatc ttcctggttc ccttatcaac gcatcgagag cgtctaccct
    13021 taaaggacgt tgaacctgaa gggagaaaaa tgatgtctca tttaaaagac ccgaccacgc
    13081 agtattacac tggtgaatat cccaaacaga aacaaccgac gccaggcatc caggcgaaga
    13141 tgacaccggt accggattgc ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc
    13201 gtaaagcact ggtgacaggg ggcgattccg gaatcggtcg cgctgccgcc attgcttacg
    13261 cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg
    13321 atgtgaaaaa gatcattgaa gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa
    13381 gcgatgagaa attcgcacgt tcgctggttc acgaagcaca caaggcgtta ggcgggctgg
    13441 atattatggc gctggtagcc gggaaacagg ttgccattcc tgatattgca gacctcacca
    13501 gcgaacagtt tcaaaagacc tttgccatta acgttttcgc gttgttctgg ctaacccagg
    13561 aagcgatccc cctgctaccg aaaggtgcaa gtatcatcac cacttcctca atccaggcat
    13621 accagccaag cccgcattta ctggactatg cggctacgaa ggcggcaatt ctgaactaca
    13681 gccgtggcct ggcaaaacag gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag
    13741 gcccgatctg gacagcactg caaatttctg gcggacaaac gcaggataag atcccgcagt
    13801 ttggtcagaa aacgccgatg aagcgtgcgg ggcaaccggc ggagctggcc cctgtatatg
    13861 tttacctggc aagtcaggag tcgagctacg tcaccgcaga agtgcacggt gtctgcggcg
    13921 gcgagcattt aggttaaaaa aatgcccggt tgtgaaaagc aaccgggcat catttttatt
    13981 tcgctttcgc agtttcttcg ccgaccagac ctattttcag gtaacccgcc tgatgcagcg
    14041 tatccattac cttcatcaac gtctcgtaat cgacagtttt atccgcacgg aagaagatgg
    14101 tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt aatcattgtt tcatcggtga
    14161 ccggatcgtt accaataaac atcgagttgt ctgctttcac cgacagataa accggttttt
    14221 ccgggcgcgg ctgcggcgtg ctggtagaag caggcaaatt caccttcaca tctaccgtcg
    14281 ctaacggtgc cgccaccata aagataatca gcaaaaccaa catcacgtcg ataaacggcg
    14341 tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt ttcgttaaga tgcatcgcca
    14401 tcggacatta tcctgcacgt aatttttgtg cgacacgaac cggatgcgca gcggcgctgg
    14461 cttccagatc caggtcacgg ctttgcagca acaatacctg cgctgcaaca tcacccagca
    14521 tcgctttaaa gccgccaatc tggcgtgcaa atacgttata gataacgacc gcaggaatcg
    14581 ctgccacgag gccgattgcc gttgctaaca gagcttctgc aatacccggc gcaacgactg
    14641 ccaggttagt ggtctgcgtt tgcgcgatac caataaagct gttcatgatg ccccagaccg
    14701 taccaaacag accaacgaac ggcgaaatcg cgccgatggt tgccagatag ccgttaccac
    14761 ggcccatttg acgacctact gcggcgaccc gacgctccag acggaagctg gtacgttctt
    14821 taataccttc gttatcgtcg ctgccttctg acagttccag ctcattctgc gcttcattga
    14881 gcaaatgcag gctcaagctt ttgctaccaa aatcagcggc gatatcgttg gcctggttta
    14941 aggaacgcgc ttcagccagc agttgctgct cgcgcttaag acgacgcttc tgattgaaga
    15001 actccacgct cttactgaag aagattgccc aggtgactac ggaggccaaa ataagcccaa
    15061 tcatcacgca cttaacgaca atatcggcgt gctgatacat accccagacg gagaggtccg
    15121 tctgcattaa attattaccc actgtgtatc tccaggacgc aagtcacaaa atctgtgcat
    15181 aataatatca aaacgacgtc gaattgatag tcgttctcat tactatttgc atactgccgt
    15241 acctttgctt tcttttcctt gcgtttacgc agtaaaaaag taaccagcac gccatttgcg
    15301 aaaattttct gctttatgcc aattcttcag gatgcgcccg cgaatattca tgctagttta
    15361 gacatccaga cgtataaaaa caggaatccc gacatggcgg acaaaaagct tgatactcaa
    15421 ctggtgaatg caggacgcag caaaaaatac acgctcggtg cggtaaatag cgtgattcag
    15481 cgcgcgtctt cgctggtctt tgacagtgta gaagccaaaa aacacgcgac acgtaatcgc
    15541 gccaatggcg agttgttcta tggacggcgt ggaacgttaa cccatttctc cttacaacaa
    15601 gcgatgtgtg aactggaagg tggcgcaggc tgtgtgctat ttccctgcgg ggcggcagcg
    15661 gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc atgtgttgat gaccaacacc
    15721 gcctatgaac cgagtcagga tttctgtagc aaaatcctca gcaaactggg cgtaacgaca
    15781 tcgtggtttg atccgctgat tggtgccgat atcgttaagc atctgcagcc aaacactaaa
    15841 atcgtgtttc tggaatcgcc aggttccatc accatggaag tccacgatgt tccggcgatt
    15901 gttgccgccg tacgcagtgt ggtcccggat gccatcatta tgatcgacaa cacctgggca
    15961 gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg tttctattca ggccgccacc
    16021 aaatatctgg ttgggcattc agatgcgatg attggcactg cggtgtgcaa tgcccgttgc
    16081 tgggagcagc tgcgagagaa tgcctatctg atgggccaga tggtcgatgc cgataccgcc
    16141 tatataacca gccgtggcct gcgcacatta ggtgtgcgtt tgcgccaaca tcatgaaagc
    16201 agtctgaaag tggctgaatg gctggcagaa catccccaag tagcgcgagt taaccaccct
    16261 gctctgcctg gcagtaaagg ccacgaattc tggaaacgag actttacagg cagcagcggg
    16321 ctattttcct ttgtgcttaa gaaaaaactc aatgatgaag agctggcgaa ctatctggat
    16381 aacttcagtt tattcagcat ggcctactcg tggggcgggt atgaatcgtt gatcctggca
    16441 aatcaaccag aacatatcgc cgccattcgc ccacaaggca agatcgattt tagcgggacc
    16501 ttgattcgcc tgcatattgg tctggaagat gtcgacgatc tgattgccga tctggacgcc
    16561 ggttttgcgc gaattgtata acattgccac ttttggacaa ttttgcagac attttattgt
    16621 gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc ggacgattca ggagtacaat
    16681 aggcagataa aggcttaaac gctgttccac aggaaagtcc atggctgtta ttcaagatat
    16741 catcgctgcg ctctggcaac acgactttgc cgcgctggcg gaccctcata ttgttagcgt
    16801 tgtttacttt gttatgtttg ccacgctgtt tttagaaaac ggcttgctgc ccgcctcatt
    16861 tttgccaggc gacagcttgt tgatactggc aggcgcattg attgcccagg gggttatgga
    16921 ttttctgcct acgattgcga ttctgaccgc cgcagcaagt ctgggctgct ggctaagtta
    16981 tattcagggg cgctggttag ggaataccaa aacggtgaaa ggctggctgg cacagcttcc
    17041 tgctaaatat caccagcgcg ccacctgcat gtttgaccgc cacggtctgc tggcgctgct
    17101 ggctggacgt tttcttgcat ttgtccgtac gttgctgcca accatggcgg gaatttccgg
    17161 tctgccaaac cgccgcttcc agtttttcaa ctggttaagc ggattgctgt gggtcagcgt
    17221 ggtaaccagt ttcggctatg ccttaagtat gattccgttc gttaaacgcc atgaagatca
    17281 ggtaatgacg ttcctgatga tcctgccaat tgccttgtta accgctggct tgttgggcac
    17341 gctgtttgtg gtgattaaaa aaaaatactg taacgcctga cgattttccc cgttcccggt
    17401 tgctgtaccg ggaacgtatt taattcccct gcatcgcccg cattcttgcc gcatcttccc
    17461 ccggcgtcac accgaagtaa cgtttaaact cacggctaaa ttgcgatgcg ctttcatagc
    17521 cgacgcgcat cgccgccgcg ctggccttca tgccgtcgtg gatgatcatc atccgcgcct
    17581 tatgcagacg gtaattcttc aaatactgca acggcgaggt gctggtgaca gacttaaaat
    17641 tatggtggaa cgccgatacg ctcatgttgg cttctgccgc cagttgctcg acgctcaggt
    17701 tttcggtgta tttattctca atccgtttca gcacgcgact aatcaggctg aagtgagtct
    17761 ggcggctgac cagtgccagt aacgcgccgc cgcaaggtcc ggtcagcacg tagtacagaa
    17821 tttcgcggat aatctgtttg ccgagaatgc gcgcatccag cggtcgctcc atcacatcca
    17881 gtaaccgctc tgccgcgcat aaaatctctt ctgataacgt ggcggagtta ataccgctgg
    17941 ctgccattga cggctgaaaa tgttcatctt cgccaatgtc catcaacagt tcctgtaact
    18001 gcaaaatatc gacattgaga cgcaaccctg ccagcggcac ctctgacgtg gcataggttt
    18061 cgcactcaaa cggcaacggc accgtcagca ggagatattc attagcgtca taacgaaaca
    18121 cgcgttcatt gatataaccg attttatgtc cggaaaagag aattatgatg ccaggctcgt
    18181 acatcaccgg tgtgcgtgcg aaaggcgtct cgccatacaa caaacgcaca tcgggcaaca
    18241 gctctgacaa actattttct ttatttttca gtttattaac tttatccgcc agcaagcggc
    18301 aaatctcttc acgtttcata tcgcgtaatt tcttaggaat aatgcggcaa tttgattgtg
    18361 cgcaattttg tagcatttct ccagcactct ggagaaatag gcaagacatt ggcagaaatg
    18421 agcattgaga ggtacggcgc tggcgaccac aatgaaaaac atcaggcaga ccgatctctg
    18481 ccctcatatt gacccagcaa agggagcaag taatgaacaa ctttaatctg cacaccccaa
    18541 cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg cgaacaaatt cctcacgatg
    18601 ctcgcgtatt gattacctac ggcggcggca gcgtgaaaaa aaccggcgtt ctcgatcaag
    18661 ttctggatgc cctgaaaggc atggacgtac tggaatttgg cggtattgag ccaaacccgg
    18721 cttatgaaac gctgatgaac gccgtgaaac tggttcgcga acagaaagtg acgttcctgc
    18781 tggcggttgg cggcggttct gtactggacg gcaccaaatt tatcgccgca gcggctaact
    18841 atccggaaaa tatcgatccg tggcacattc tgcaaacggg cggtaaagag attaaaagcg
    18901 ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg ttcagaatcc aacgcaggcg
    18961 cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt ccattctgcc catgttcagc
    19021 ccgtatttgc cgtgctcgat ccggtttata cctacaccct gccgccgcgt caggtggcta
    19081 acggcgtagt ggacgccttt gtacacaccg tggaacagta tgttaccaaa ccggttgatg
    19141 ccaaaattca ggaccgtttc gcagaaggca ttttgctgac gctgatcgaa gatggtccga
    19201 aagccctgaa agagccagaa aactacgatg tgcgcgccaa tgtcatgtgg gcggcgactc
    19261 aggcgctgaa cggtttgatc ggcgctggcg taccgcagga ctgggcaacg catatgctgg
    19321 gccacgaact gactgcgatg cacggtctgg atcacgcgca aacactggct atcgtcctgc
    19381 ctgcactgtg gaatgaaaaa cgcgatacca agcgcgctaa gctgctgcaa tatgctgaac
    19441 gcgtctggaa tatcactgaa ggttcagacg atgagcgtat tgacgccgcg attgccgcaa
    19501 cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct ctccgactac ggtctggacg
    19561 gcagctccat cccggctttg ctgaaaaaac tggaagagca cggcatgacc caactgggcg
    19621 aaaatcatga cattacgctg gatgtcagcc gccgtatata cgaagccgcc cgctaagctt
    19681 tttacgcctc aaactttcgt tttcgggcat ttcgtccaga cttaagttca caacacctca
    19741 ccggagcctg ctccggtgag ttcatataaa ggaggaacgt atggctaatc caaccgttat
    19801 taagctacag gatggcaatg tcatgcccca gctgggactg ggcgtctggc aagcaagtaa
    19861 tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg ggttatcgct cgattgatac
    19921 cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc ctgaaaaatg cctcagtcaa
    19981 cagagaagaa ctgttcatca ccactaagct gtggaacgac gaccacaagc gcccccgcga
    20041 agccctgctc gacagcctga aaaaactcca gcttgattat atcgacctct atttaatgca
    20101 ctggcccgtt cccgctatcg accattatgt cgaagcatgg aaaggcatga tcgaattgca
    20161 aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc cagatccatc acctgcaacg
    20221 cctgattgat gaaactggcg tgacgcctgt gataaaccag atcgaacttc acccgctgat
    20281 gcaacaacgc caactacatg cctggaacgc gacacacaaa atacagaccg aatcctggag
    20341 tccattagcg caaggtggga aaggtgtttt cgatcagaaa gtcattcgcg atctggcaga
    20401 taaatacggc aaaacacctg cgcagattgt tatccgctgg catctggata gcggcctggt
    20461 ggtgatccca aaatcggtca caccttcacg tattgccgaa aactttgatg tctgggattt
    20521 ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc gatcagggca agcgtctcgg
    20581 tcccgatcct gaccagtttg gcggctaacg tgcaaatttt cccggtggcg gtaatgttcc
    20641 gctaccggac ttttcagaaa ccatttattc ccctcgcgtc ccgcccgttg ctactcttcc
    20701 ttgttcagga atgccaaata taaggacatc atcatgcaga gccggaagct cttaaaagaa
    20761 caactcatct atatccggga taaacgcaac ggagaggtga aaaacagatg aaaataatac
    20821 ttctgttttt agcagccctg gcaagtttta ccgtacacgc acagcccccc tcacagaccg
    20881 tagaacaaac agtccggcat atttatcaga actataaatc agatgccact gccccttatt
    20941 ttggtgaaac cggagagcgg gcgataacat ctgcgcgtat tcaacaggcg cttaccctga
    21001 acgacaatct tacgctgccg ggcaatattg gctggctgga ttatgatccg gtttgtgatt
    21061 gtcaggattt tggcgatctg gtgttagaaa gcgtagcgat aacccaaact gacgccgatc
    21121 atgccgatgc cattgtgcgc tttcgtatct ttcaagacga taaagaaaag acctcgcaga
    21181 cactgaaaat ggtggcggaa aatggtcgtt gggtcattga cgatattgtc agcaatcatg
    21241 gcagcgtctt acaagcggtt aatagcgaga atgaaaaaac actggccgct atagcttcgt
    21301 tgcaaaaaga acagccggaa gcctttgttg ccgaactctt tgaacatatt gctgattata
    21361 gctggccgtg gacgtgggtg gtttccgact cttaccgcca ggcggtcaat gccttctata
    21421 aaaccacctt caagacggcc aacaatcccg atgaagatat gccaatagaa cggcaattta
    21481 tttacgacaa tccgatctgt tttggcgaag agtcgttatt ttcacgcgtt gatgaaattc
    21541 gagtcctgga gaaaaccacc gattccgccc gcattcatgt tcgttttacg ctgaccaatg
    21601 gcaacaacga agagcaagaa ctgattttac agcggcgcga aggtaagtgg gaaatcgctg
    21661 attttatccg cccgaacagc ggcagcctac ttaagcagat tgaggcaaaa accgccgcca
    21721 gattaaaaca atgagctgaa ttaaataaca attagccgga ataataaata aaagggaaca
    21781 ctatatgaaa acgattttca ccgtgggagc tgttgttctg gcaacctgct tgctcagtgg
    21841 ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat gtgcaaacat taaatgccaa
    21901 aatcgcccgg cttgagcagg atatgaaagc attacgccca caaatctatg ctgccaaatc
    21961 cgaagctaac agagccaata cgcgtcttga tgcacaggac tatttcgact gcctgcgctg
    22021 cttgcgtatg tacgcagaat gataaaaaaa tccccggcag cattctgtta ccggggatat
    22081 tttttaacgt ccaaccgccg ctttagggcg tttcttcgca ccagcattca ccggacgaga
    22141 ttgcgtagac gacgcttttt ttgccgtagc aggcgtctga cgctgggtcg ccatcggcgt
    22201 atgtttagtc aacgccggac gggtattgcg gttctggcga cgagcctcac gcatctcttc
    22261 aatggttggc gcaggcacta agcaatcgcg acggctgcca atcagatgct ttttgcccat
    22321 cgcttccagc gcctggcgga ttaacggcca gtttgccgga tcgtggtaac gcaacaacgc
    22381 tttatgcagg cgacgctgct tgtcgccctt cggtacgaag acgtcttcac tcttataacc
    22441 aatcttcgcc agcgggtttt tgccggtgta atacatggtg gtcgagttag ccagcggcga
    22501 cggatagaag ttctgcacct ggtcgaggcg gaagcgatgc tttttcagcc atagcgccag
    22561 attcaccata tcttcatcac gcgtacccgg gtgcgcggag atgaaatacg ggatcagata
    22621 ctgttcttta cctgcctgtt tcgagtaagt atcgaacagc tctttaaagc ggtcatagct
    22681 gcccatgccc ggcttcatca tcttcgataa cggcccttct tcggtatgtt ccggggcaat
    22741 cttcagataa ccgccaacgt gatgggtcgc cagctctttg atatagcgcg gatcttctac
    22801 ggcgatgtca taacgcacac cagaggcaat caggatcttt ttaatgcctt tcagatcgcg
    22861 cgcacggcga tagaggttaa tcgtcggttc gtggttagtg tccatatgcg gacaaatatc
    22921 cggataaacg cacgacaaac gacggcaggt ttgctcagcg cgtggcgatt tgcaacgcaa
    22981 catatacatg ttggcagtag gcccaccgag atcggaaatc acgcccgtaa aacctggaac
    23041 agtgtcgcgg atcgcttcga tctcattaat aatcgaatct tcggaacggc tctgaataat
    23101 gcgcccttcg tgctcggtaa tagaacagaa agagcagccg ccaaagcagc cacgcataat
    23161 gttgaccgag aaacgaatca tttcgtaagc cggaattcgg gcattaccgt aagccggatg
    23221 cggcacgcgc ttgtacggca gcgcaaagac gctatccatc tcttcggtag aaagcggaat
    23281 agcaggcggg ttgatccata catagcggtc gccatgtttt tgcatcaatg cgcgggcgca
    23341 gcctgggtta gtttcgtggt gcagaatgcg cgaagcatgg gcgtacagca ctttatcgcc
    23401 cttcactttc tcgaaagaag gcagcaacac gtaggttttt tcccacggtt tcgggcgtgg
    23461 tggctgcacg gttacagctt tggcttcctg ctttttcgga gctaccggtt tgttatccgc
    23521 gcacggcaaa tcttcgccat acggatgcgg gattgggtcg attttccctg gggtatcaag
    23581 acgggtggaa tccacaccgc tccagccagg caacgcctct tttacgataa tcgcggtatt
    23641 acgcacatcg cggatttcac taatcggctc gcccatcgcc agacggtgcg ccacttccac
    23701 cagcggacgc tcgccgttac cgaacatcag catgtcagct ttggaatcca ccaacaccga
    23761 acggcgcacg gtatccgacc agtaatcata atgcgcggta cggcgcaggc tggcctcaat
    23821 accgccgagg atcaccggca catctttcca cgcctcttta caacgctggg tataaaccag
    23881 tgtggcgcga tccggacgct tacccgcgac gttatccggt gtgtaggcat cgtcatgacg
    23941 taaacggcga tcggcggtat agcggttgat catcgagtcc atgttgccag cagtaacacc
    24001 gaaaaacaga ttcggtttac ccagacgcat aaagtcgtct ttactgctcc agtctggctg
    24061 ggcgatgatc ccgacgcgaa agccctgcgc ttccagcata cgaccgcaaa tcgccatccc
    24121 gaagcttggg tgatcgacat acgcgtcgcc agtaaccaaa atgatgtcgc agctatccca
    24181 gccaagttga tccatctctt cacgtgacat cggcaaaaac ggtgccggtc caaaacaggc
    24241 ggcccagtac tgcggccagg agaacaggtc gcgatccggt tggatcaggg agatagagct
    24301 cattttgctt ccagaaatgg taaaaaaata atcaaaggcc ggggattata cgctgtatca
    24361 cgcgcggaaa tgaagtgatg ttgatgaacg gtggcgaaat accctggtga ggtttttcac
    24421 tgcccgactt gggcaagcac atttaaggct ggcggtatcg tggcaactgc cgctactatt
    24481 tatgccgatc agcaacgttc aaatcatatt gcacgacgat cggctatccg aggacaccca
    24541 cagtaaggca aggtatggaa caggttatta caaagcgccg ctattacgat attggtctac
    24601 agattgaaga gctgctgtat tcaggcgtat ttaaggccgg agaacgctta ccttcagaac
    24661 gtgaactgag cgagcggttt aataccagcc gaactactat tcgtgaagcc atcattatgc
    24721 tggaactgaa aggcgtattg aatgtgaagc aagggtcagg gattttcttt gtcgactcca
    24781 ccgataagct caaccagaaa tcattaatgc cctattcaga aattggcccg tttgaactgt
    24841 tacaggcacg tcaggtaatt gagagcaata ttaccggttt tgctgccagc cagatcagct
    24901 ttaacgagtt acaagagttg aagaaaatca ttggattaca ggaaaaagcc atcgctgcgg
    24961 aaagtgataa atttgaagac ctggatcacc gatttcatag cattattgcc gaagcgacgc
    25021 aaaatcgggt actcatcaaa caagccgctg aattatggcg ggcagtgcgc acagaaaacc
    25081 cccgctggaa gaaacttaac tacaagtacc tgcatgaaaa gcatttgcgt ctgcaatggc
    25141 tggaagatca ccgggctatt ttcctggctc tgcaacagaa agattcagag ctcgccaggg
    25201 aagcgtcctg gagacatctg gaaaacagca aaaatgaact gatcaaaatc ttcaaacaag
    25261 acgcttccat cagcgatttt gatgactttt tcttcgctcg ttaaccttca ttagtccaga
    25321 gctggcggga aaaataacgc cggctctgcc ttccgccctc cactggtaca cgctctcctt
    25381 tcaattggtc taataaatcg actatcatca gatatcttag attagcggca attatcccaa
    25441 aaactgactt ctaaaaaact tccctttcat tttattcata taactcattg ataaaaatat
    25501 aaataacgcc ttgttgttct gtttcgtact tttacctttc tctgcaagcg tgatgcatct
    25561 ctctgttttt ggtctagtga attatgtaaa ttggtctaaa cacaagccac caggagtaag
    25621 agcataatgt cagggaacga aagtatcgct gccgcccact atgatcaggt aaccacctgg
    25681 cctcgggacg ggttacaggc agatattgtc catattggtt ttggcgcttt tcatcgcgga
    25741 caccaggctg tctacacgga tctcactaat caactttcgg acacccgctg ggggatcttt
    25801 gagatcaacc tgtttggtga tgctcaactg atcgaaaact taaatgcgca aaatgggctg
    25861 ttttcggttg tggaaacatc tgcatcgcaa tctaccttac gccttgtgcg ttcggtggct
    25921 ggcggtattc atacccccag agatggcatt gccgcagcaa tccataaact ggctgaacct
    25981 caggtaaaaa tcgtttcatt aaccatcacc gagaaaggtt attgcctcga tccgcaaaca
    26041 cgttcacttg atctcaccaa tggattaatc aaacacgatc tacaaaaccc gggtgcgcct
    26101 ctgtcagcta ttggcgtgat cgtctgtgct ttgcaacaac gtaaagcggc aggactcgcc
    26161 gcttttagtg tgctctcctg tgacaacttg ccagacaatg ggcatctgac gcgcaatacc
    26221 gttctcggtt ttgcccgaca actggaccag cccctggctc agtggatcga agaaaatgtc
    26281 tcatttccag gcacgatggt agatcgcatt gttccggcaa tgactgaatc gcaattcgcc
    26341 ttactggaaa ctaaaaccgg ttatgccgat ccctgcggga tcgtctgcga atcatttcgt
    26401 cagtgggtga tcgaagataa ttttgtgagc ggacgaccgg aatgggataa agccggcgcg
    26461 atgtttgtca gcaatgttca gccttatgaa gagatgaagt tacgcatgtt aaatggtagc
    26521 cattcatttc tggcttataa cggctcactg gctggctatg agtttatctg gcaatgcatg
    26581 gaagacgcta attttcgttc cattacccac caactgatga ttaatgaaca agcccgaaca
    26641 cttaatccag acttaaatat caacatccag gaatacgccg acctgttaat tgaacgcttt
    26701 agcaaccgta acgttgcaca ccgtaccggg caaatagcca tggacggttc acaaaagctt
    26761 ccccagcgtg cactgacgcc ctggctgaaa ttgcatcagc aaaaacaaaa caatgctgtt
    26821 ctgtcactgc tcgttgctgg ttggttgcat tatgtcattg atgttgttga gaaaagccag
    26881 tctgtcgctg atccaatgaa tgaccaattt cagacgctaa taaaggaaca acaagacgca
    26941 tggcaacagg cgctcgcatt actgcacctt agcgccatat ttggtgattt aagcaaccat
    27001 cagccattta taaatgaaat aaaaatcgcc tttgcgaata taaaaaacaa aggcatcaag
    27061 gccaccatca gccaattatt atcggatgag cagaaatgaa aacattaatt tgccagcagc
    27121 ctggcgttat ggaatatgtg gaaaaggata ttcccacacc agcagataat gaagtgctgt
    27181 taaaaatcaa agctgtgggt atttgtggta ctgatattca cgcttttgcc ggcagacagc
    27241 ctttttttag ctacccacgt gtattaggtc atgaaatatg cgccgaagcg gtttcgcgag
    27301 gcagccagtg ccaaacagca caatcaggcc agcgctattc cgtcatccca tgcattccgt
    27361 gtggcgagtg cgcagcctgt cgggaagaga aaacgaactg ctgcgaacgt gtttcgctgt
    27421 atggcgtgca tcaggatggg ggttttagtg agtaccttgc ggtacgtgaa gacaaccttg
    27481 tgcctctccc tgacgaggtc agcgacagtg ccggagcatt ggttgaatgt ttcgccattg
    27541 gtgcacatgc cgttcgtcgg gcagagatca aggctgaaca aaacgtactg gtgattggcg
    27601 ccgggccaat aggtctggct accgcagcca tcgccagggc taaaggggcg catgttgttg
    27661 ttgctgatat tgattgtcaa cgtcgccagc acgttgtggg taatctggat attaatgcct
    27721 tcgatcccac acagaaagat tttattgccg cgcttcgtga ggcttttgga ggcgaactgg
    27781 cttgcgtagt actggatgcg acgggaaata aagcttcaat gagtcaggat gtaaatctta
    27841 ttcgtcatgg cggcaaaatt gttttcattg gtttatacat tggtgaactt gttattgacg
    27901 atccgacctt ccataaaaaa gagacaacgt tactcagcag tcgcaatgcc acacgggaag
    27961 attttgcgtt ggtgattgaa ctgatgcgca gcaataaaat tcacgaaaat ttaatgaaaa
    28021 accaggagtt cgatttcttt agtgttggcg aagattacca gcgtaacgtt gtagaaaata
    28081 aaaatatggt caagggtgtg atcacttttt aagggaacaa atatgacaac tgtttatgtc
    28141 agtgaagaaa atttaaaatc actggtgcac cacaaattac ataccgccgg gctggatacc
    28201 gacaccacac aacaggtgac agatgtttta gtacatgccg atattaccgg tgtgcattca
    28261 catggtgtta tgcgtgtcga acattattgc acccgccttg ctgccggagg gttaaataaa
    28321 gccccgcagt ttagcattga gcaaatttca ccgtcagtgg ccattctcga ctctgacgac
    28381 ggaatggggc attccgcatt aattagcgcc actgagcacg ctattaaact ggcccagcaa
    28441 gagggtctcg gttttgttag cgttaaaaac acgtctcact gtggtgcgtt atcttatttt
    28501 gcggagatga tcaccaacaa agggttggtt gctatcgtaa tgacgcaaac cgatacctgt
    28561 gtggctcccc atggcggcgc cgagcgcttt ttgggaacta accccatcgc ctttggtttc
    28621 ccggtggaaa acagccatcc gatgattgtt gatatggcga ccagtgccac ggctttcggc
    28681 aaaatacttc atgcaaagga aaccggaaaa catattggtg aagggctggc gatagataaa
    28741 aacggttatg gcaccactga tccgtataag attgaaaacc tgctaccttt cggccaacac
    28801 aaaggttcag gcattgcact ggctattgat gcactgactg gcgtgctgat gaatgcgaat
    28861 tttggcaacc atattgttcg catgtatggt gattatgaca agatgcgtaa gctggcaagt
    28921 ttggttattg ccatcgatcc gaaaaagctc ggcaatcctg tttttgcaaa aactatggcg
    28981 aaaatggtca cggagctgca tgccgttaaa ccggctcctg gtgtcgaaaa agtgttagcg
    29041 ccgaacgatc cgcaaatgca ctacaaagaa aaatgccaac aggaaggtat tccggttccg
    29101 gcaggaatat tccattatct ggcagagaat taatcactat tttttagtat aacgatagaa
    29161 aagcgctgta tttatcgttt ctgttatatt aaccaggagc aggatatgaa agcacttcgt
    29221 cctctgacgg catcgcttat gctgttaacc agttgtttat taatatctaa taccacactg
    29281 gcaaaaacaa cattaaaact gagtcataat caggataaaa gccatgctgt ccataaagca
    29341 ttaagctatt tagcggataa aaccaaagag tattctaatg gtgaattagt tattcgcatt
    29401 tacccaaatg cgacattagg taatgagcgt gaatcactgg aattaatgaa ctccggtgca
    29461 ttacagatgg ttaaggttaa tgccgcatca ctagaatcat ttgctccgga ttacagcctt
    29521 ttcagtctgc cgttcttatt ccgcgaccgt gatcactatt atcgtgttct gcaaagtgat
    29581 ttaggtaaaa aaatacttaa ttcatcagaa agcaaaggtt ttgttggtat tacgtactat
    29641 gacggcggtg cgagaagttt ttattccaat aaaccgatta caaaacctga agatttagcg
    29701 ggaatgaaaa tcagggttca acaaagcccc agcgccattg caatgatgaa agcactcggt
    29761 ggtgtcgcta ccccgatggc gcaaggcgaa ctctacaccg cacttcagca aggagtggtt
    29821 gatggcggag agaacaatac cgttgtctat gccgatatgc gtcacgccga agtcgcaaaa
    29881 gtctattcac gtgatgaaca caccatggta cctgatgtgt taattatcag caccgacgtg
    29941 ttgaataaac ttggtgataa agagcgcacg gcattattaa aagcagccga tgagtcaatg
    30001 atgcagatga aggacgtcat ttggcctgcg gctgaaaaag aagcctacga caaaatgaaa
    30061 gggatgaacg caacagttgt tgatgttgat aaatccgctt tcaaagaacg cgtcaaaccg
    30121 ttatacgatg aattcaaggc gaaagatgca caatcagcca aaaaccttga gctagtagaa
    30181 agtatgtaac cctaatgggc aggtaattcc tgccttttca tggaagactt atgcgaacac
    30241 tcactcatat tcttaataag atccttgccg gatgttgctg cattattctc gccattatgg
    30301 tgttctgcgt aacctggcaa gttattgcac gttttatttt taacgctccc agcactgtgc
    30361 ttgatgagtt cacccaaatt ttatttatgt ggatgatttt acttggcggt gtatatactg
    30421 cggggttaaa aaaacatctg gccattgatt tgttggctca aaaacttcct gctgcatctg
    30481 ttctgacgct tgacagtttt atccaaatta tcattacggt ctttgcggtt attttcatga
    30541 tctatggtgg gaatattgtt gttgaaaaag cacaacatgt tggacaaatc tctccagtat
    30601 taaaatggcc aatggataaa gtatattggg ttatgcctgt cagtgggctt attctggtct
    30661 ggtattcagt aatgaatatt attgacaact atcgtaaacg gaattctcat taacgaggga
    30721 aatatggaat ttgaatatat ctaccccgtc ttaattttat ttggcagttt tgccgtcatg
    30781 ctggcaatag gtgtgccaat tacttttgcg attggtcttt cttcgctgtt atctattatt
    30841 actgccttgc cacccgatgc cgccatttct gttatttcac aaaaaatgac tgtagggctg
    30901 gatggcttta cgctattagc cattcccttc ttcgtgttag ccggaaacat tatgaatacc
    30961 ggtggtatag ccagacgact ggttaacctg gcgcaagcat tagttgggcg tcttcctggc
    31021 tcactggctc attgtaatat cctcgcgaat acactgtttg gtgcgatttc aggttcagcc
    31081 gttgcgtcag ccgcagcggt aggtgggatt atgtcaccac tgcaagaaaa agagggatat
    31141 gatccggcgt tttcagcagc ggttaatatt gcctctgccc ccattggcct gatgatccca
    31201 ccgagcaatg tgttgattgt ttattccctc gccagtggcg ggacttctgt cgctgcgttg
    31261 ttcttagctg gatatctgcc gggcatcctc accgctgttg ccttaatgtt tgtagctgca
    31321 ctttatgcac gacgtcacca ttacccagtg gccgaacgca tcaattaccg tcagttttta
    31381 caggtattcc gcgaatcaat tcctagcctg atgcttattt ttatcattat tggcggtatt
    31441 atcgcagggg tatttacgcc cacagaagca tcagcaatag cggtaattta tagtttagcc
    31501 ctggcgatga tttaccggga aatcactctt aagaagctca atgatattct gttagattcg
    31561 gtagtaacca gttcaattgt tctgttactg gtaggctgtt caatgggaat gtcatgggcc
    31621 atgacgaatg ctgatgttcc tgagttgatc aacgaactga ttacccgcgt ttcggataac
    31681 aaatgggtca ttctgtttat tatcaatatc attctgttga tcgtcggtac ctttatggat
    31741 atcacgccgg cgatcctgat atttacacct attttcctgc cgatcgccca gcatctgggg
    31801 attgatccga ttcacttcgg cataattatg gttttcaact taaccattgg cctttgcaca
    31861 ccacctgtcg gcaccattct gtttgtgggg tgcagcattg gtaaggtcag tatcgacagg
    31921 gcaataaaac cattactgcc gatgtttctg gcattgtttg tagtaatggc aattatttgt
    31981 tatttcccgc agcttagtct gatgctgcca ggattatttt cgacctgata cacaatgaag
    32041 actgcatact gagtaatttt caatgcccca ccactctgtg gggcattgaa ttggtccatt
    32101 acggtaccgg attgaccaac agttgcccaa tcgagccacg gtccgccatt tccagcgtct
    32161 gactgttgaa gtagaacggg aagtgcgccc aggaaggctg accgaaataa acaagcagct
    32221 ccacttgtcc gtcaacccaa acggtatctt tccagcctct gtcttccggg aacggcatcg
    32281 cgccattcac gttacggatc tggaacatca cgccttcaat atggaacgct tgcggctcgt
    32341 ccgcgcgcac cgtccagcgt tcccacgttc cttgctgcgc agtaacatca atacggttga
    32401 cgtcccacaa ctgcccatta atccccgggt catcacccag actaatatcg cgactgcgaa
    32461 tcggcgaacc ggccatgatt tcagttggca gcaagcgcat cggaagactg tcagtgacca
    32521 gcggcagaag gccggttggg cgcagcgtta gcaccagcgt agaaaccaga atgctggatg
    32581 gctcaaagaa gccacgaata cgatcaacaa tgctcgccgc ttcgccacag gtaatcgaca
    32641 cttcatcacc gttgctcata tccaccagaa tctcacgacg ctcgcccggt gccagcgaaa
    32701 gttgcttcac cgatacagga gcgggcagga atccctgatc gccagaaatc acatgtaacg
    32761 ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc aacagacgca
    32821 agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta accagtagcg
    32881 tatcgccaac aaagccgccg cttccaggtt cgttatattc cggcgtacca aagttatcca
    32941 gccgtttatc ctgaataatg acaggaaaat catccacacc ataatggttg gggataggca
    33001 gcgatttact gacttcatct tccaccagcc acattccggc aaggccgtta taaacctgct
    33061 gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatgg
    33121 gcagtacggg tgcccagtca gcatttggcg acatcatccg tgccggaccg cccatcagcg
    33181 ggccaggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cggttgctgt
    33241 aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat
    33301 tgattcccca aaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag
    33361 tcataaacag cggttgccca cggcgagatt caagcagcgg cggaacgggt agcggttgct
    33421 gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg
    33481 cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta
    33541 ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc
    33601 ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg
    33661 acaatgggca gccaactcgc gaacctgatc tttgccatac tgactgacgt caattggcgg
    33721 cagcatttcg acaatcacca gaccgttgtg tagccgatta agattaatct tattcgaagt
    33781 tgtagagacg cacacgggta taatcgggac gcccgccgca attgccgcgt gaaatgcccc
    33841 ggtcttgaac ggcagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat
    33901 ggaaatacgg cgttttttga agtgattcac tacttccgca atagtgccgt gagctttagt
    33961 gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg
    34021 gatccacagc aagctctttt tacctaccgt cactgtcggc ggctgcacaa tgttcgatgc
    34081 cgtcaccatg tcataattgt tctggtggtt agcaatatag atagcattgc cgtagctttc
    34141 agcgtctgca ggtttgcggc actccacttt caggccgaaa agcggcgcaa gacggccaaa
    34201 catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga
    34261 gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa agatatatag
    34321 cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa atcaggttag
    34381 ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc atgaataaac
    34441 aacggtttca agggaatttt tatcgtttcc ggggattgaa tacgctgccg gatgacgact
    34501 taacgtttca tccggtgttc cttgcaagcg ggaggaaaca gcgccctccc cggcatatta
    34561 ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg atctcaacac gatcgatacg
    34621 ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg ccagtgactt tctgtagctc
    34681 ttccggacgc agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc tttgcggcgg
    34741 cagaacgtac agttgcgcca ggccatcctc gccacgcgcg gcttctgccg atggaatgtt
    34801 gataatcttg ttgcctttgc ccttcgacag ctgcggcaga tcgcttaccg ggaacatcaa
    34861 catacggcct gcctgagtga ttgccagcag catatcggaa gcatcttcaa tcaccaccgg
    34921 cggcataaca tgggcatttt ccggtaaggt gatcaaagcc ttacctgcac ggttacgcgc
    34981 caccagatcg ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca tcagcagttt
    35041 ctgatcgtcg ctttccatca gcatatggtc aacggtcgct ccaggcggca acgttaattt
    35101 gccggtgagc ggctcgccct gaccacgcgc cgacggcagc gtaatcgggt cgatggcata
    35161 gctacgaccg gtggaatcaa caaacactac cggttggttg ctcttacctt tcaccgccgc
    35221 tttgaagcta tcgcccgctt tataattcag gcccggcgcg tcgatatcat ggcctttagc
    35281 gctgcgtacc cagcccatct gcgacagcac aatggtgaca ggttcagacg gcagcatgtc
    35341 gtgctcgctc atcgctttcg cttcttcgcg ttcctgcaac ggcgaacgac gttcgtcacc
    35401 gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg ttattcattt tacgctcgga
    35461 agccaaaatg ccctgcaact ggtcgcgctc tttttccagc tcactctgct caccgcgaat
    35521 cttcatctct tccagtttgg caagatgacg cagtttcagt tcgaggatcg cttccgcctg
    35581 ggtttccgta aggccaaacc gcgacatcag cgccggtttc ggttcatctt cattacgaat
    35641 gatctcaatc acttcgtcga tattgagaaa cgccaccagc aaaccttcga ggatatgcag
    35701 gcgcttgagg actttctcca gacgatagtt cagtcggcgg cgcacggtat cgcggcggaa
    35761 caccagccat tcggagagga tttccagcag gtttttcacc gccggacgac catccagacc
    35821 gatcatattg aggttaatgc gatagctctt ttccagatcg gtggtagcaa agaggtggtt
    35881 catcacctga tccatatcca cgcggttgga acgcggcaca atcaccagac gggtcgggtt
    35941 ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc agctttttgt tgcgcatttg
    36001 cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga tgcggcaatg cgctgataac
    36061 caccgcgcca tcttctttct tccacaccgc gcgcatacgc actgaaccac gtccgttctc
    36121 gtagatttta cgaatctcgg cgcgcgaagt gataatttcc gcttcagtcg gataatccgg
    36181 cccctgcacg atatccagca gttgatcgag agtggttttc ggctggtcga ttaatgcgat
    36241 tgccgcctga gccacttcac gcaggttatg cggtggaata tcggtcgcca tgccgacggc
    36301 aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg gcaggcagca ttttcggctc
    36361 ctgcaaagtg ccgtcgaagt ttggcaccca gtcagccgtc ccctgcccca gctcgctcaa
    36421 tagcagctcg gaatatttcg acaaccggga ttcggtgtaa cgcattgccg cgaacgattt
    36481 cggatcgtcc ggcgcacccc agttcccctg accatcaacc agcggataac ggtaagagaa
    36541 cggctgcgcc atcaggacca tcgcttcata acaggcacta tcgccgtgcg gatggtattt
    36601 acccagtacg tcaccgacgg tacgggccga ttttttaaat ttggcgctgg cattcaggcc
    36661 cagctcagac atcgcataca caatgcggcg ctgaacaggt ttcagaccat caccaataaa
    36721 cggcaacgca cggtccatga tcacgtacat ggagtagttt aagtaggcgt tttccgtaaa
    36781 ttcatgtagc gcaaggcgct ctgccatatc gctcattaat tctgattcct caacttattc
    36841 gccagccttc aaaggggcaa tattgccgga gatactacat tatctgccgc gtcgagtcac
    36901 aggagatcag cgtgaatagc gccgccggac gtgctccgac ggcgataaag gcatcaatgt
    36961 gccttgatat acaactcttt gctgtagaca taatcctggg gattatgcag cggaaaaccg
    37021 ccaacgtagg gtttaagcag tttgattaac ggctgatagt agatgggaat cagcggtgcc
    37081 tgctggttga tgatcacttc tgcctgctga tacaacgcat tacgctttgt cgcatcagtg
    37141 atctgcgtgg cctggtttag taaggcgtca tactgcgcat ttttccagtg accgacgttt
    37201 tcttcactat cgcttttgag cgtgttcagg aagctggagg catcattgta cgtcgcatcc
    37261 cacgactgcc gagacagcat aaaatcaccg gctcgtcggg catcaagata ggttttccac
    37321 tccattgtgc gcagcgtcac ctgtgcaccc agccattttt tccattcgga agacaacgct
    37381 atcgcggtct tttcatgcag atcgtacttg ttgtagaaca gctcaaagcg tagcggatga
    37441 gaggcgtcgt atcccgcctg tttcagcaag acttttgcca tcgcgacgcg ctcactcatt
    37501 ggcttttgca gttcatcgaa cgtcgtcgcg ctaaagcctt ttacctctgg cggcgtcagc
    37561 gtggttgcgg gcgttctcaa ccccagtacc ttttgcgcaa taagctgtcg atcaaccgta
    37621 agatatagcg cccgacgcac ccgcacatcg ttaaatggcg gtttctcaag gttaaagttg
    37681 taatattcgc tgttcagacg cgggataatt cgtagctcgc caggcagtga tttttcaatg
    37741 gcgggaattt gctgcgccgg aacccaggtg agatcgacct ctcccgcacg atagcggtta
    37801 tagccggtga ccgaattatc cagcgccaga tactcaacct gttgcaatac tgtatgttgc
    37861 gcatcgcggt actttggatt tttgcgtgca gtaatctttt cgttaactac ccatttatca
    37921 agcacaaagg caccgttgta aaccatgttc tctggcttac tccagctatc gccatgttta
    37981 gcgatgacat gatgaggaac cgggaacagc gtcggccagg cgagcatcgt cgtgaaccaa
    38041 ggaactggct gctcaagcgt aacttcaaga gtacgatcat ccgtcgcttt gacacccagc
    38101 gatgtaacat ctgctttacc cgcaacaata gccgcggcat tgttaatgtg cgcctgtgcc
    38161 agatagccag caaaagggct tgccgttttc gggtcaaccg cgcgctgcca gccgaggaca
    38221 aaatcctctg ccgtcagagg ctgaccgtct gaccactgca aaccgctacg cagatggaaa
    38281 atatagcgct tgccgtcgtc aagtatctcc cagcgttcag cctgagcggg ctgcacctgg
    38341 ccttcaccgt ccatccatac cagaccttca aacagatcca gcacaatctg cgcggcagta
    38401 ttctcctcta ccttttgcgg gtcgagcgtg cctgggtcgc tatgattgtt gtaacgaaag
    38461 acttgttgcg gggcgagcgg tgtgttggcg ggaacgtcag cagcgtagag aggagccgca
    38521 cttaccaggc tgaccagcca taataaattt cgcgtataca tggtattcct taccggagaa
    38581 attataagcc cgacaacaat acgcctgtgg cattgtttgc tcaaacttat cgcgagtgat
    38641 ttggctcaca ctccggtaaa gattccccat cacgccaacg gcacataaat atccgtttcc
    38701 agtcgccgct ctgtcatccc ttcggcaaga ttggtgtagt gaaacaggat cggggctttg
    38761 cgcatttttt cgccgcttgc aggtagccag tggcgaatga tgccccatat cgtatgtgaa
    38821 atatcgtcca gctcgccaac atggcgggca acagcataac gcccaccggt aagttcgcca
    38881 ttactcacgc cataacgatt atcggggatc ggttcgctaa cgctgccgca gatatcaaag
    38941 cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt
    39001 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacgctgtag
    39061 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    39121 ataatattta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    39181 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    39241 ttcaacagag ttccagtctt tatcgacttc cccctgaatc tcaaccgtat ctttcggcgt
    39301 caccgtcacg ccgttccagc gtttgtggtc gatatcaaca ttgatagtac cactggcatc
    39361 tttgaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgta gggttaccca
    39421 ggtgtcgtca cgcagggatt ttgcgctttc tacggtcgtt acgctgccgt tcggcccctg
    39481 gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaacctccct gctctgctgc
    39541 cataaccggt gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    39601 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    39661 aagggaaaaa aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag
    39721 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    39781 gattggcgac ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg actggtttac
    39841 acaaggtcgt cagggaaaag aagcgctata tagcgcgcct tatgatgcgg tgatcctgga
    39901 tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag aaaaaggtca
    39961 gcgtgagcct gtattgatcc tgactgcgcg cgatgcgctg gcggaacgtg tagaagggct
    40021 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    40081 gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggcaa
    40141 cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacgctgaa
    40201 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    40261 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    40321 agtgcatgtg catcatctgc gacgcaaact cggtagtgat tttattcgta ccgttcatgg
    40381 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgagtcagg
    40441 ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc
    40501 tggaaacaaa caacggataa cgtcgatgag ttgttcgaca cccaactgat gctgtttgcc
    40561 aaacggttaa gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg
    40621 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc
    40681 cacgacggca gaatggtcct taacgatggc gataacggag aagatattcc ctatagctat
    40741 cagcgggaag gttttgctga cgggcaactg gtcggtgaag acgatccctg gcgttttgtc
    40801 tggatgacct cacctgatgg caaatatcgc atcgttgtgg gtcaggaatg ggaataccgt
    40861 gaagacatgg cgctggcgat tgttgccgga caattgatcc cgtggctggt cgcactgccg
    40921 attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg
    40981 gcgctggcac tacgtatgcg tgacccagac tcggaaaaac cactaaacgc gacgggcgta
    41041 cccagcgaag tgcgcccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatacg
    41101 atgatggttc gtgaacgacg ctttacctcc gacgcagctc acgaacttcg tagcccgtta
    41161 acggcgctga aagtgcaaac cgaagttgct cagctctctg acgatgatcc gcaggcgcgg
    41221 aaaaaagcgc tgctccaatt acattccggg atcgatcgcg ctactcgtct ggttgatcaa
    41281 ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    41341 cttgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa
    41401 attgacgtgc gactgacact caaagccaac ggcatcaaac gcaccggaca accgctattg
    41461 ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acaaggtagc
    41521 gtggtagacg tcacgctgaa tgccgataat ttcatcgtga gggataacgg ccccggtgtg
    41581 acaccagagg cattggcgcg aattggcgaa cgctactatc gcccacccgg acaaaccgct
    41641 accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaagctgca tgacatgaat
    41701 gttgaatttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg gtaagggtaa
    41761 gacttttgct aaattcgcaa aagactttgc acattttgct aatttcacca taccgctctg
    41821 tgacgtacta tagtcggcaa acgtctcacc ttgaggttaa aaaatgagca acatcctgat
    41881 tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga
    41941 agtcgcggat ggcacactgc gcgacctcgg gcatgatgtc cgcattgttc gcgccgacag
    42001 cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca
    42061 gatgcctggc tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg atgatgtatt
    42121 caccgaaggt cacgggactc tgtatgccag cgatggtcgt acccgcaaag atccatcaaa
    42181 aaaatacggt tccggcggcc tggtacaggg caaaaaatat atgctttctc tgacctggaa
    42241 cgcaccaatg gaagccttca ccgaaaaaga tcagttcttc cacggcgttg gtgttgacgg
    42301 tgtgtacctg ccgttccata aagcaaacca attccttggc atggaaccgc tgccgacatt
    42361 tatcgctaat gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa
    42421 gcatcttgtg gaaatttttg gttaactaga gctctggctt tagaaggagt taaccatgat
    42481 tacagtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cagtgttgga
    42541 tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc
    42601 aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat
    42661 gattgagcag tgggaaagca tcgcgcatct tgaagcgcat ctgcaaaccc cgcacatgaa
    42721 agcatatagc gaagccgtaa aaggtgacgt gctggagatg aatattcgta tcctgcagcc
    42781 ggggctttga catttctaaa aagaggccgg tgctgctacc ggtctcagcc cagattagcc
    42841 attctttctt cccgttttca gtcctcgatg gaaatcgtta attcgcttaa tggttttaac
    42901 cgcacgctgc ggcactgcgg aagctaccga ccagacaatc atttccagac acaacaatac
    42961 cgtaccatgt agcggcgttt tccctttttc atccccgcgc ggaacatgaa tcacgatact
    43021 ggcgtctttg ctaaaacgtg aatccagcgc gtttgtcagc aaaataacag ggatccccag
    43081 ccgcctcgcc tcacgaagcg ttgtcagccc ttcccggtgc gcagattttt gcgccatcat
    43141 gatcagaaca tcgccacgtt gaagcgcgat cagttgctcg gcaaggccaa tacccgtacg
    43201 gtttagtgcc gtggcaggca aacctatgcg gttgaatagt cgtgcggtat agtccgccag
    43261 aatacccgaa gcgccaatgc caaaaattgc gacttgccgt gcctgcacca acaacgctac
    43321 agcttgcgca agcgcgtggc ggttttcagg ctctgacagg acattacagg tatacaaatg
    43381 cccttcaaga acaaagtcga ttgcggtgtt aacatcgctc gtcaagttac tcaccgtcgt
    43441 agacatcttt tcgctggaac taagggctgg accaagccac tgttccagag tgcgtttcag
    43501 atcacgcagc ccggcaaacc ccagggcctg tatagccctg ataactgtgg catcggaagt
    43561 gttcaacgtg gccgcaattt ccatcgccgt ttgctcgagt accacttcac gatgctcatt
    43621 aatgtagctg gcgacagctc ttaacccagg cgttaactga ctcgcgcgag tgcgaaaacg
    43681 ctcaccaaaa atatcaaccc gcgctttatc tttctggatc atttagcctc cggtagaggt
    43741 cgccctgcaa tttgtgattg tatctgcgca gccatcagcc ccagagatgc aaaggagtta
    43801 gaaattgcct cttcacgaga aatcagcaca taacgcccgg aacgacaggc agtcagaaac
    43861 tgacaccatc ccggcatgac tttttccatt gctgccagct catcctgagg tttaccaccg
    43921 gtgtcaccgc gccaggttgc aaaaacaaaa tcggcatcca gttcaggtaa acgctccgcg
    43981 ctcacatcca ttcgcccacc ttcaggaatg ctctcaatca gcggcggaaa acgaaaaccc
    44041 gcatcgcgta atacccgccc cagcgaatga tagctatgca tcacattaat ttttccctgg
    44101 tttgcctgaa tcaccgatac cgttattttt tggctgtcca gcgtcgcctt cagcgcattg
    44161 atttgtgcct gatagcggcg ttcaagtatc gccagctgcg actgcgttcc tgtaagttct
    44221 gccagcttgc gatagatttc tggcgcaccc cctttgagat gatcaatgct caccgtgggc
    44281 gcaatttttt caagttgttc aatgggcgta ttacgggtcg gttctgtgat gatcaggtca
    44341 ggctttgccg ccacaatcgc ttcaatatca atatcagcgg taccaataaa ggcgatcgat
    44401 gaattatcaa agtcgacacc tgtcaataac gcgccagatc gaatgaaatg gctaccgtca
    44461 ggccgagttc gcccgtggct ggcgacagga ggtacaccga gctcaatcag aggaatagtg
    44521 atatcaagat cgtgtaaaga gacaattctc tttggatgag caggaacagt gactttgcgc
    44581 cctaaatcat cagtaaatac ctgaacaggt tctgttgcac gggaaaaaaa tgacaacagt
    44641 atcagcagag aaaaaaataa acgcatataa ctcccgttaa aaactgttcc tgcgctgcca
    44701 gagcagtagc aaaaaaaatg gtccaccaat cagtgagata atgattccgg caggcagttg
    44761 caaaggcaaa aaagccaaac gaccaatgct atccgccaga ataactaaca acgcccccga
    44821 taccgcgctc cccagcagta atgtcgtctg cccgccacgt agaataaaac gtgatatatg
    44881 gggtgctatc aatccgacaa aaccgatgtt accaacacac gaaacgcaga cggctgtcag
    44941 gattatgggt gcggcgacac gtaacaacgc cagacgagaa ctattcaccc ctaacccggt
    45001 tgcaacctga tgaccaagca gagcgatatc cgcagtcctg gcggtgaaca gcaataaaag
    45061 agcggctggc aacatccaac aggctgaaat accgactaac atccagttag ctgcatgcag
    45121 gcttcccgat aaccacatca atgcagtttg tacatctcga acatctgcag tggtcataaa
    45181 tacaccgata ccggcggcga aaaaccagga aacaccaata ccaatcagaa caaatcgcgg
    45241 gcgggaaata tcgcgcgcac aaaaaatcac gattaaagcc accaacagac caccagcaag
    45301 accagccact ggacgccaga acattcccag cattggaaac tggaaaatca gccacagcac
    45361 cgccacgcta catccttctt tgacgccgat taaaccagga tcggcaaggc cgttacgcgc
    45421 aatggattgc atcgcggccc ccgccatacc taacatggcc ccgcatagca atgccatgat
    45481 gactctcggc aagcgaatat cctgcacaat ataacgtgcg tctgtgctta cgttttctgg
    45541 agcaaacaac gctcgtccta cttccgatgc cggaacagag aaagaaccct gcatcagacc
    45601 gaatatcagt attccaaccg caagcaggaa cagcccacca agcaacagca gatttttagg
    45661 gcgcaccagt gtcgaaaact taccaacacg tagtgggcgt agcccgacgt tcattatccg
    45721 actcatttga acatcctcgt cgccataatg acaaaaacag gcgcaccaac taacgccgtc
    45781 atgacaccgg tcgctaactc atggggcgtg aaaagcgtcc gggcaataat atctgccagt
    45841 aataacaaaa gcgcgccgac acaagctgaa agagggagca gaacgcgcag atccgctgat
    45901 accagacgcc ggacaatctg cggtactagc aggccgacaa aaccaatggg gccagcaatc
    45961 gatacggctg cgccacagag caacgcgatc gcaagcaggg taaatgttcg cgtgcggagt
    46021 acagagacgc ccagcccctg cgccatgcga tcgcccagtg ccaacatgtt aagggagggt
    46081 gcaaggtaaa gggctaacac aaatcccccc agagaaaacc aggcggacgt acccagcgtc
    46141 gcccaatcct gccccgctaa atcacctgct agccatgtcc gcatcgccag cagtgtttgt
    46201 tcgtcaagaa tcaggattgc agccgttatt gaggacacga acgccgatag cgccacgccg
    46261 cataaagtaa ctttcattgg cgttacccct gaacgccctg cggaggaaag cagcaatatc
    46321 aacaggaaca ataacgcgcc ccccgtagat gccagcagtg ggcgtccaac cggaaaggcc
    46381 agtccaagcg cactggcggc aacgacagca agagcggctc cggcatttaa gcctaaaata
    46441 tgtggctccc ccaatgggtt acgaatgaca gcctgcaaca aagcaccagc aacaccaaga
    46501 gaagcaccgg taagcaatgc tgccgctaaa cgcggcaaac ggagtcttac aattacgttg
    46561 tgatcgaagt tttgcggatc aaaatgaaga aatgcctgca acaccgtttg tggtgcaata
    46621 aatcgggcac caatccctgg atataccacg catcccacca gcagtaaggc gaggaaaatt
    46681 acagtaacca gccctgtgcg catcatactt tttctacacc acgaaacggc atgaagaacg
    46741 gctttcccgt cagaggattt attgatgcgt gaacatcaac gtcaaagacc gcttttacca
    46801 gttcaggcgt gcaatgctcc ccctcattca acacacgcac cactttcccc tggcgtaaaa
    46861 atatcaaggt atcaccataa ttgacggcaa aattcagatc atgcaacacc acgacgactg
    46921 tacgcccgtg atgacgcgtt aaatcatgta ataactcaag aatttcaaac tgataacgta
    46981 aatcaagcca ggttgtgggt tcatccagca ggatataagg cgtcttttgc gccaacacca
    47041 tcgcaatcca gcaacgctgg cgctgcccac cagagagttt ttcaacaggt aaatgtgcaa
    47101 actcctgcgt accggttaat ttcagggcct cttccaccgc ctgctcatcg gcgtcgctcc
    47161 attgacgtat gaaattttgc cagggaaaac gcccacgcga caccagttca taaacggtta
    47221 agccctccgg caataatggc gactgcggca agatcccaag tctacgggat aacgctttcg
    47281 ttggttgctc gtgaatcgct ttgccatcaa gcaatatcga accgcccatg ggttgcaaga
    47341 tacgtgcaat ggtgctgagc aacgtcgact ttccacagcc gtttgcccca accagaacgg
    47401 tcattttttc tgtgggaacg gagaaagaaa caccatcaac aatgatcttc ttgtgatacc
    47461 cggcagaaac attgtcgagg atcagcccct gcccttttgc gttatgtgcc acgtgtacgc
    47521 caacttaatc aaagacaaac aaaaataaaa ctcattctcc tttgagagtt tgacgccaca
    47581 aaatgtagta gtcaagcagt taaaagc
//
LOCUS       DAZKHK010000019        46991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKHK010000019 DAZKHK010000000
VERSION     DAZKHK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.019"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            210..1487
                     /gene="waaA"
                     /locus_tag="ACHOS5_001139"
     CDS             210..1487
                     /gene="waaA"
                     /locus_tag="ACHOS5_001139"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP9775993.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1495..1974
                     /gene="coaD"
                     /locus_tag="ACHOS5_001140"
     CDS             1495..1974
                     /gene="coaD"
                     /locus_tag="ACHOS5_001140"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9775994.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(2013..2822)
                     /gene="mutM"
                     /locus_tag="ACHOS5_001141"
     CDS             complement(2013..2822)
                     /gene="mutM"
                     /locus_tag="ACHOS5_001141"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP9775995.1"
                     /translation="MPELPEVETSRRGIEPHLAGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2920..3087)
                     /gene="rpmG"
                     /locus_tag="ACHOS5_001142"
     CDS             complement(2920..3087)
                     /gene="rpmG"
                     /locus_tag="ACHOS5_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9775996.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(3108..3344)
                     /gene="rpmB"
                     /locus_tag="ACHOS5_001143"
     CDS             complement(3108..3344)
                     /gene="rpmB"
                     /locus_tag="ACHOS5_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9775997.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3561..4229)
                     /gene="radC"
                     /locus_tag="ACHOS5_001144"
     CDS             complement(3561..4229)
                     /gene="radC"
                     /locus_tag="ACHOS5_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9775998.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4401..5621
                     /gene="coaBC"
                     /locus_tag="ACHOS5_001145"
     CDS             4401..5621
                     /gene="coaBC"
                     /locus_tag="ACHOS5_001145"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP9775999.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKALLGQLL
                     LDEIVTRYDEKNRR"
     gene            5599..6057
                     /gene="dut"
                     /locus_tag="ACHOS5_001146"
     CDS             5599..6057
                     /gene="dut"
                     /locus_tag="ACHOS5_001146"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9776000.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6164..6760
                     /gene="slmA"
                     /locus_tag="ACHOS5_001147"
     CDS             6164..6760
                     /gene="slmA"
                     /locus_tag="ACHOS5_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP9776001.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6797..7438)
                     /gene="pyrE"
                     /locus_tag="ACHOS5_001148"
     CDS             complement(6797..7438)
                     /gene="pyrE"
                     /locus_tag="ACHOS5_001148"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9776002.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7504..8220)
                     /gene="rph"
                     /locus_tag="ACHOS5_001149"
     CDS             complement(7504..8220)
                     /gene="rph"
                     /locus_tag="ACHOS5_001149"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9776003.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8347..9210
                     /gene="yicC"
                     /locus_tag="ACHOS5_001150"
     CDS             8347..9210
                     /gene="yicC"
                     /locus_tag="ACHOS5_001150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9776004.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVAKVRAHMPEILQWQRERLIAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9462..10079
                     /gene="yicG"
                     /locus_tag="ACHOS5_001151"
     CDS             9462..10079
                     /gene="yicG"
                     /locus_tag="ACHOS5_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP9776005.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10076..11758)
                     /gene="ligB"
                     /locus_tag="ACHOS5_001152"
     CDS             complement(10076..11758)
                     /gene="ligB"
                     /locus_tag="ACHOS5_001152"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP9776006.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARTKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLSAQQITGFEP"
     gene            12016..12639
                     /gene="gmk"
                     /locus_tag="ACHOS5_001153"
     CDS             12016..12639
                     /gene="gmk"
                     /locus_tag="ACHOS5_001153"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9776007.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            12694..12969
                     /gene="rpoZ"
                     /locus_tag="ACHOS5_001154"
     CDS             12694..12969
                     /gene="rpoZ"
                     /locus_tag="ACHOS5_001154"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9776008.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            12988..15096
                     /gene="spoT"
                     /locus_tag="ACHOS5_001155"
     CDS             12988..15096
                     /gene="spoT"
                     /locus_tag="ACHOS5_001155"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9776009.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            15103..15792
                     /gene="trmH"
                     /locus_tag="ACHOS5_001156"
     CDS             15103..15792
                     /gene="trmH"
                     /locus_tag="ACHOS5_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP9776010.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFRKIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            15798..17879
                     /gene="recG"
                     /locus_tag="ACHOS5_001157"
     CDS             15798..17879
                     /gene="recG"
                     /locus_tag="ACHOS5_001157"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9776011.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEVKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGLH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(17913..19118)
                     /gene="gltS"
                     /locus_tag="ACHOS5_001158"
     CDS             complement(17913..19118)
                     /gene="gltS"
                     /locus_tag="ACHOS5_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP9776012.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            19398..20789
                     /gene="xanP"
                     /locus_tag="ACHOS5_001159"
     CDS             19398..20789
                     /gene="xanP"
                     /locus_tag="ACHOS5_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP9776013.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            20910..22619
                     /gene="yicH"
                     /locus_tag="ACHOS5_001160"
     CDS             20910..22619
                     /gene="yicH"
                     /locus_tag="ACHOS5_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9776014.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(22663..23343)
                     /locus_tag="ACHOS5_001161"
     CDS             complement(22663..23343)
                     /locus_tag="ACHOS5_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFP9776015.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(23324..24256)
                     /locus_tag="ACHOS5_001162"
     CDS             complement(23324..24256)
                     /locus_tag="ACHOS5_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9776016.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTSAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(24304..25164)
                     /locus_tag="ACHOS5_001163"
     CDS             complement(24304..25164)
                     /locus_tag="ACHOS5_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9776017.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLHAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSFGKASLY"
     gene            complement(25245..26096)
                     /locus_tag="ACHOS5_001164"
     CDS             complement(25245..26096)
                     /locus_tag="ACHOS5_001164"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9776018.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGAIGKA"
     gene            complement(26108..27199)
                     /locus_tag="ACHOS5_001165"
     CDS             complement(26108..27199)
                     /locus_tag="ACHOS5_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9776019.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSFW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            complement(27224..27538)
                     /locus_tag="ACHOS5_001166"
     CDS             complement(27224..27538)
                     /locus_tag="ACHOS5_001166"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9776020.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(27556..28026)
                     /locus_tag="ACHOS5_001167"
     CDS             complement(27556..28026)
                     /locus_tag="ACHOS5_001167"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFP9776021.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(28053..29606)
                     /locus_tag="ACHOS5_001168"
     CDS             complement(28053..29606)
                     /locus_tag="ACHOS5_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFP9776022.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(29901..32219)
                     /gene="yicI"
                     /locus_tag="ACHOS5_001169"
     CDS             complement(29901..32219)
                     /gene="yicI"
                     /locus_tag="ACHOS5_001169"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP9776023.1"
                     /translation="MKISDGNWLIQPDLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYVTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(32229..33611)
                     /gene="yicJ"
                     /locus_tag="ACHOS5_001170"
     CDS             complement(32229..33611)
                     /gene="yicJ"
                     /locus_tag="ACHOS5_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP9776024.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSVTISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            33904..33998
                     /locus_tag="ACHOS5_001171"
     tRNA            33904..33998
                     /locus_tag="ACHOS5_001171"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33938..33940,aa:Sec,seq:tca)
     gene            34297..35487
                     /locus_tag="ACHOS5_001172"
     CDS             34297..35487
                     /locus_tag="ACHOS5_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9776025.1"
                     /translation="MALTDIKVKTAKPKEKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKILAAGGDPGVAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLACALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIALFKQLEPITKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSKQ
                     ESQG"
     gene            35924..36967
                     /locus_tag="ACHOS5_001173"
     CDS             35924..36967
                     /locus_tag="ACHOS5_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9776026.1"
                     /translation="MNDRLRFEVNNNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISE
                     TSYINKLRRLAQQEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFNG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHKQAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVRHNFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPGILPF
                     SAAGMKHSSIQHQ"
     gene            complement(37111..38784)
                     /locus_tag="ACHOS5_001174"
     CDS             complement(37111..38784)
                     /locus_tag="ACHOS5_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9776027.1"
                     /translation="MYSSATVCFTVSAGQKYRFYSIIMKINLPFIHWSRISNDKRLVL
                     SVVVFTLSNILVSSGAHLIYRILFFTLALLIIKAMKSLTLRFILAFSFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIRMFRMYVVYVIAFIILFLLFFCSAINKMS
                     SLPSEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARTDNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSADTVLHHDVRHYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGSRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFTMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPALGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTKPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNELLE"
     gene            complement(38850..39047)
                     /locus_tag="ACHOS5_001175"
     CDS             complement(38850..39047)
                     /locus_tag="ACHOS5_001175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776028.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVVLGML
                     FLLIMIAGGMCRSLLSRKKTS"
     gene            complement(39187..39453)
                     /locus_tag="ACHOS5_001176"
     CDS             complement(39187..39453)
                     /locus_tag="ACHOS5_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9776029.1"
                     /translation="MKLHPPFPCNQCGACCRHVNRAGETQCLDRGDGICRHYQTDSHL
                     CAIYDKRPQICRVEDQYLLNYQSQYSWQEFIALNQAACLILNKL"
     gene            complement(39450..41021)
                     /locus_tag="ACHOS5_001177"
     CDS             complement(39450..41021)
                     /locus_tag="ACHOS5_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFP9776030.1"
                     /translation="MNVEKFDWTEELHQTMVKSLVTSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGEYDSHHYHSHENYIATNREGKRAHQAGTLTDTYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLINLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFSAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFRRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAALNHFSLHLDSVNSEIERGLLLREEVSRRGIELPF
                     ESGNVASVRDWLNKR"
     gene            complement(41068..42147)
                     /locus_tag="ACHOS5_001178"
     CDS             complement(41068..42147)
                     /locus_tag="ACHOS5_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HFP9776031.1"
                     /translation="MALPFIILGVAAGTAAFGGKKAYDGYQKKSEAEDILEKAKERYE
                     EHKEKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIQNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACDVARAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DISVSDEVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMETDDNGMN
                     VINKDELDDVLASSVVKFDDFSSRS"
     gene            complement(42196..43092)
                     /locus_tag="ACHOS5_001179"
     CDS             complement(42196..43092)
                     /locus_tag="ACHOS5_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFP9776032.1"
                     /translation="MADSTRNRSAERLIDILVELQNYGVVSRHNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKESLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAICNNNVCQIAYAGKTRIVHPYKLTN
                     QRSIWYLLATEENRLKSFSLAKIKWLDIKKEKFAKSDEIQSLVSESCDPWVSDKTFDV
                     VLFIHSNIAHYFLRRDLLPYQQLLHKHDNGIMLSCKAAHKNQIIPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            complement(43516..44253)
                     /locus_tag="ACHOS5_001180"
     CDS             complement(43516..44253)
                     /locus_tag="ACHOS5_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFP9776033.1"
                     /translation="MKKVIAVSALTMAGMFSAQTLADESKTGFYVTGKAGASVMSLSD
                     QRFVDGEGAWADKYKGGDKSDTVFGTGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESVGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSTSASKYENNLAWSLGVGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
     gene            44572..45033
                     /locus_tag="ACHOS5_001181"
     CDS             44572..45033
                     /locus_tag="ACHOS5_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP9776034.1"
                     /translation="MCKLTREEEYSIISRTMTNIRSLRMYVRGVDFEQLLDMQERLNA
                     VIEERRKDAEREAAERAEREQRRQELLQLIAGEGFSLEELLDRPEDTPKSRKKTLPKA
                     PPKYQFEENGETKYWSGRGRAPKPIDEAMKAGRSLEDFRINKNSNDITDEQ"
     gene            45308..45490
                     /locus_tag="ACHOS5_001182"
                     /pseudo
     CDS             45308..45490
                     /locus_tag="ACHOS5_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(45488..45604)
                     /locus_tag="ACHOS5_001183"
                     /pseudo
     CDS             complement(45488..45604)
                     /locus_tag="ACHOS5_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45634..45822)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_001184"
                     /pseudo
     CDS             complement(45634..45822)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            46595..>46991
                     /locus_tag="ACHOS5_001185"
     CDS             46595..>46991
                     /locus_tag="ACHOS5_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HFP9776035.1"
                     /translation="MKEIDIKLKIVEFLLNSEPADTYLAAEVRFSFGSRRADIVSVSS
                     DIATVYEIKSEKDSVERLVYQIDSYKEYFDYCYIVCVDKNLDAVRKKISTNVGIIVVD
                     DINVKRIRKAKRIARQEKLCLASTIPVNEL"
BASE COUNT        12135 a        12150 c        11039 g        11667 t
ORIGIN      
        1 agcaaccata ttcttttact gttgctctgc gatttcttcc tgcacgtact gcagaaatca
       61 atgaatcgca taaaaataca aaaaaagtat agatgttcag caagaattgg cgggtaaatt
      121 gctatttaag tcaaaaacgg gaaaagtaat ggtaaagcca cagctaaata catagaatcc
      181 ccagcacatc cataagtcag ctatttacta tgctcgaatt gctttacacc gcccttctct
      241 accttattca gccgctgatc tggatacggc tctgggtgcg cggacgtaag gctccggcct
      301 atcgaaaacg ctggggtgaa cgttacggtt tttaccgcca tccgctaaaa ccaggcggca
      361 ttatgctgca ctccgtctcc gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcac
      421 tgcgtcatcg ttatcctgat ttaccgatta ccgtaacaac catgactcca accggttcgg
      481 agcgcgtaca atcggctttc gggaaggatg ttcagcacgt ttatctgccg tatgacctgc
      541 ccgatgcact caaccgtttc ctgaataaag tcgaccctaa actggtgttg attatggaaa
      601 ccgaactatg gcctaacctg attgcggcgc tacataaacg taaaattccg ctggtgatcg
      661 ctaacgcgcg actctctgcc cgctcggccg caggttatgc caaactgggt aaattcgtcc
      721 gtcgcttgct gcgtcgtatt acgctgattg ccgcccaaaa tgaagaagat ggtgctcgtt
      781 ttgtggcgtt gggcgcaaaa aataatcagg tgaccgttac cggtagcctg aaattcgata
      841 tttctgtaac gccgcagttg gctgctaaag ccgtgacgct gcgccgccaa tgggcaccac
      901 accgcccggt atggattgcc accagcactc atgaaggcga agagagtgtg gtgatcgccg
      961 cacatcaggc attgttacag caattcccga atttattgct catcctggta ccccgtcatc
     1021 cagaacgctt cccggatgcg attaaccttg tccgccaggc tggactaagc tatatcacac
     1081 gatcttcagg ggaagtcccc tccaccagca cgcaggttgt ggttggcgat acgatgggcg
     1141 agttgatgtt actgtacggc attgccgatc tcgcctttgt tggcggttca ctggttgaac
     1201 gtggtgggca taatccgctg gaagcggccg cacacgctat tccggtattg atggggccgc
     1261 atacgtttaa ctttaaagac atttgcgcgc ggctggagca ggcaagcggg ctgattaccg
     1321 ttaccgatgc cactacgctt gcaaaagagg tttcctcttt actcaccgac gccgattacc
     1381 gtagtttcta tggccgtcat gccgttgaag tgctgtatca aaaccagggc gcactacagc
     1441 gtctgcttca actgctggaa ccttacctgc caccgaaaac gcattgaggt tgttatgcaa
     1501 aaacgggcga tttatccggg tactttcgat cccattacca atggtcatat cgatatcgtg
     1561 acgcgcgcca cgcaaatgtt cgatcacgtt attctggcga ttgccgctag tcccagtaaa
     1621 aaaccgatgt ttacccttga agagcgtgtg gagctggcac agcaggcaac cgcgcatctg
     1681 gggaacgtgg aagtggtcgg gtttagtgat ttgatggcga acttcgcccg taatcaacac
     1741 gctacggtgc tgattcgtgg cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg
     1801 gcgcatatga atcgccactt aatgccggag ctggaaagtg tgtttctgat gccgtcgaaa
     1861 gagtggtcgt ttatctcttc atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc
     1921 acccatttcc tgccggagaa tgtccatcag gcgctgatgg cgaagttagc gtagcgttta
     1981 tgccggatgg tatgccatcc ggcgcgcatc aattacttct ggcactgccg acaataaaac
     2041 gttgcccgct gcgcatgttt agtcgccaca atcggcgtac cgcacacccg acacggctca
     2101 ccttttcgcc cgtaaacctg caattcctga gcgaaatagc ccggtttacc atcactttgc
     2161 agaaaatctt tcagcgttgt accgccctgc tcaatcgaac gcagcaacac cgctttaatc
     2221 acccgagcta acaattcaca ctccgccagc gacagtgatg acgccagccg atccggatgg
     2281 atccccgccg caaacagcga ttcgctggca tagatattcc ctacgcctac caccagcttg
     2341 ttatccatca gccacggttt aatcgccgtt tttttcttcg cgcacttctg atgcaggtac
     2401 tcaccgttga aatcgtcact aagcggctcc ggtccaagat gggccagcac gttgtgccct
     2461 tccagctctt tggtccatag ccaggcacca aagcggcgcg gatcggtgta gcgcagcact
     2521 ttgccgttgc tcatcaccaa atccacatgg tcatgctttt cagggggaag ttcttctgga
     2581 aggatgcgca ggctgccaga catccccaaa tgaatgataa tccagccctc aggcagctcc
     2641 agcagcagat atttagcccg gcgctgcacg ctaagcactg gttggtcgct taaacggtag
     2701 atctcttctg aaaccggcca gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt
     2761 gcaccagcga gatgcggttc tatgccgcga cggctggttt caacttcggg taattcaggc
     2821 atagcatctc caggaatgaa cagatgcagt caatatgggg gcaagcagat aacaaaaaac
     2881 cccgccgggg cgaggttttt tgttacatca aagcggaaat tatttgattt tcgcttcttt
     2941 gtagatcacg tgctggcgaa caactggatc gaattttttc agttccagtt tttccggctt
     3001 agtacgtttg ttcttcgtag tggtatagaa gtgaccagta ccagcagaag aaaccagctt
     3061 gattttctca cgaatacctt tagccatgat ttatttcctc taagtactta gtacttttcg
     3121 ccacgggcac gcagttcagc cagaactgta tcgatgcctt ttttatcgat tacacgcata
     3181 cctttagcag atacgcgcag ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga
     3241 gagtgcagat tcggcaggaa acggcgttta gtcgcgttca gtgcgtggga acggttgtta
     3301 ccggtcaccg gacgcttgcc agtaacttgg cagactcggg acatgtctat tctccaaaaa
     3361 tcaaattagc tcgagcttcg tatggggtat tggcgcctcg tcaggcttta cagcccggtc
     3421 atcgcagttc tatgtgaact ctcgattgcc aggcccaaat gccaaacccg agattctcaa
     3481 aggtggcgta gtatacgctg actcagcgat gtgctcaagt cccgaacaga caaagatccc
     3541 gaaggatcgc gcatagcggg ttaaatccag ccgcgttcgg caaaagaaac atactctcca
     3601 cgcccaatca cgatatggtc gagcacgcgt aaatccatga actgacaact ctttattatc
     3661 cgttcagtaa tgagtttatc cgctttactg ggttcagcac aacccgaagg gtgattatgc
     3721 gcaaggatca gcgccgaggc gtttattttt atcgcttcgc ggataatttc ccgaggatgg
     3781 acttcaacat ggtttagcgt gccggaaaaa agacggctat gcgttataac cctgtgttgg
     3841 gagtcgagaa agatcaccat aaagatctcc cgctcctcgc ccgtgagctg gctttgtaaa
     3901 aattccctcg tcatctccgg gctgagtaaa gggctttctt cacgcatccg tacgttgtag
     3961 taacgccgcg ccagttcggc aatccccttt aactgggcga atttcgccac gccaattcca
     4021 tgaacgccac taaattgctc atattcagag gttaacaagc cataaagaga gccgaaattc
     4081 tccagcatct cttttgccag ggttaataca tctttaccgc gcgttccggt acgcagaaat
     4141 agcgccagca gctcgacatc cgttaaggcg ctaataccaa acttcagcat tttttcgcgc
     4201 ggcatcaaca gctgtgcatt gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt
     4261 catagccgtt cctttaaatc gacggccagt tttcaatcct ggaaagcgcc tcgcaaagtg
     4321 aatctcaggt gaaggcacga caacaaaagt attgtgataa aatcgccaac ttctggtgtc
     4381 acccaacagg aaaaatcatc atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg
     4441 gcggtattgc tgcctataaa acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg
     4501 atgtccgcgt agccatgacc gaagcggcaa aagcctttat caccccactt agcttgcagg
     4561 cggtttctgg ttatcccgtt tccgacagtc tgctggaccc ggcagccgaa gccgctatgg
     4621 gccatattga gctgggtaaa tgggctgatt tagtgattct cgcccctgcc acggcagatt
     4681 taattgcccg tgttgctgcc ggaatggcga atgacctggt atcgacgatt tgtctggcta
     4741 cacctgcgcc tgtagccgtg ctccccgcca tgaaccagca gatgtaccgt gccgctgcca
     4801 cgcagcataa tttagaggtg cttgcctcac gtggtttgct catctggggg ccagacagcg
     4861 gcagtcaggc ttgtggtgat atcggcccag gacgaatgct cgatccgtta accattgtgg
     4921 atatggcggt agcgcatttt tcgcccgtca acgacctgaa acatctgaac attatgatta
     4981 ccgccggtcc gacgcgtgaa ccgctcgatc ccgtgcgtta tatctctaat cacagctccg
     5041 gcaagatggg ttttgctatc gccgccgccg cagcccgtcg tggcgcgaac gtcacgctgg
     5101 tatcaggtcc ggtttcacta ccgacgccac cgtttgttaa ccgtgttgat gtgatgaccg
     5161 cgctggaaat ggaagccgcc gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt
     5221 gcgccgccgt ggcggattat cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg
     5281 ccacgcaggg tgatgaatta acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg
     5341 ttgccgcact aaaagaccat cgaccctacg tcgttggatt tgccgccgaa acaaataatg
     5401 tggaagaata cgcccggcaa aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg
     5461 atgtttccca gccaactcaa ggatttaaca gcgacaacaa cgcattacac cttttctggc
     5521 aggacggaga taaagtctta ccgcttgagc gcaaagcgct ccttggccaa ttattactcg
     5581 acgagatcgt gacccgttat gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt
     5641 gggaaggaat ttccgctccc gacttatgcc acctctggct ctgccggact tgacctgcgt
     5701 gcctgtctcg acgacgccgt agaactggct ccgggtgaca ctacgctggt tccgaccggg
     5761 ctggcgattc atattgccga tccttcactg gcggcaatga tgctgccgcg ctccggattg
     5821 ggacataagc acggtatcgt gcttggtaac ctggtaggat tgatcgattc tgactatcag
     5881 ggccagttga tgatttccgt gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc
     5941 gaacgcatcg cccagatgat ttttgttccg gtagtacagg ctgaatttaa tctggtggaa
     6001 gatttcgacg ccaccgaccg cggtgaaggc ggctttggtc actctggtcg tcagtaacac
     6061 atacgcatcc gaataacgtc ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg
     6121 ggtgccgcct ggcaagtgct tattttcagg ggtattttgt aacatggcag aaaaacaaac
     6181 tgcgaaaagg aaccgtcgcg aggaaatact tcagtctctg gcgctgatgc tggaatccag
     6241 cgatggaagc caacgtatca cgacggcaaa actggccgcc tctgtcggcg tttccgaagc
     6301 ggcactgtat cgccacttcc ccagtaagac ccgcatgttc gatagcctga ttgagtttat
     6361 cgaagatagc ctgattactc gcatcaatct gattctgaaa gatgagaaag acaccacagc
     6421 gcgcctgcgt ctgattgtgt tgctgcttct cggttttggt gagcgtaatc ctggcctgac
     6481 ccgcatcctc actggtcatg cactaatgtt tgaacaggat cgtctgcaag ggcgcatcaa
     6541 ccagctgttc gagcgtattg aagcgcagtt gcgtcaggta ttgcgtgaaa agagaatgcg
     6601 tgagggtgaa ggttacacca ccgatgaaac cctgctggca agccagttgc tggcattttg
     6661 tgaaggtatg ctgtcacgat ttgtccgcag cgagtttaaa taccgcccga cggatgattt
     6721 tgacgcccgc tggccgctaa ttgccgcaca gttgcagtaa tatgacgccg gatgactttt
     6781 catccggcga gtttcgttaa acgccaaact cttcgcgata ggccttaacc gccgccagat
     6841 gttccgccat ttctggcttc tcttccaggt aagcaatcag gtctttcagg gtgatgatag
     6901 agatcacttt gcagttgtaa tcacgctcaa cttcctgaat tgccgaaatc tcgccgcgcc
     6961 cgcgttcctg acgatcaagc gaaatcaaca cgcctgcaag cgtcgcgcca ttggcctgaa
     7021 taatttccat cgactcgcga atcgccgttc cggcggtgat cacatcatct accagcatta
     7081 cgcgtccttg taacgcgcta ccaaccagat taccgccttc accgtggtct tttgcctctt
     7141 tgcggttaaa gcagtacggc aggtcaaggt catgatgctc cgccagcgcc acggcggtag
     7201 tggtggcaat cgggatccct ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg
     7261 aatccaccaa tgcttcagcg taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa
     7321 acagcccggc gttgaagaaa taggggcttt tgcgcccgga tttcagcgtg aactcgccaa
     7381 actttaacac ctgcttgcta agcgcaaatt caataaactg gcgctgatat ggtttcatgc
     7441 cttcgctcct catcttactt ttctacagac aaaaaaaagg cgactcatca gtcgccttaa
     7501 aaatcagttt gccagcgccg ccttctgcgt cgctacaatg gattcgattc cccctcgggc
     7561 cagcgccaac aaggtgagta actcttcgtg ggtgaacggt tcgccttctg ccgtcccctg
     7621 cacttcaatg atacgcccgt cttcggtcat cactacgttc atgtcggtct ctgcggcaga
     7681 gtcttcaaca tattccagat cgcaaaccgc ttcgccgttc acaatcccga cagagaccgc
     7741 ggctaccatc cctttcattg gattagtttt cagcttgccg ttttccacca gcttctgtag
     7801 cgcatctgcc agcgccacgc aggcacccgt aatcgaagcg gtacgcgtgc caccatcagc
     7861 ctgaagcacg tcgcagtcca gcgtaatggt gaactcaccc agtgctttca aatctactgc
     7921 cgcgcgaaga gcacgggcga tcagacgctg gatttccatt gtgcgtccac cctgcttacc
     7981 tttcgccgct tcacgcgcgt tacgggtatg ggtagaacgc ggcagcatac cgtactctgc
     8041 ggtgatccag ccctggccct gacctttcag gaagcgcggc acgccttctt caatagaggc
     8101 ggtacacaat actttggtat cgccaaattc gaccagcacc gagccttctg catgttttgt
     8161 atagttacga gtcagggtaa cgggacgcac ctgattattg ctacggcctg ctggacgcat
     8221 attgaaatct ccggcttgaa acaaatgtgg ctgcgcatta tacggacttc cggcggttat
     8281 tcctatcctg acaaggcatc gatggctata atccttccac ctctcctttt ataaacagga
     8341 acgtctatga tccgcagtat gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg
     8401 agcgcaacct gggaaatgcg ctcggtaaac cagcgttatc tggaaactta ctttcgtctg
     8461 ccggagcagt tccgtagcct tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg
     8521 cgcggtaaag tggaatgtac tctgcgctat gagccagatg ttagcgcgca aggtgagctg
     8581 atcctcaacg aaaaactggc taaacagctg gtaactgccg cgaactgggt aaaaatgcag
     8641 agcgacgaag gagaaatcaa cccggttgat attctacgct ggccgggcgt gatggcagcc
     8701 caggagcaag atctcgacgc gattgcagct gaaattctcg cggcgttgga tggcaccctg
     8761 gatgacttta tcatcgcgcg cgaaaccgaa ggtcaggcac tgaaagtgtt gattgagcag
     8821 cgtctggaag gcgtcaccgc cgaagtggcc aaagtccgcg cccatatgcc ggaaatcctg
     8881 caatggcagc gtgagcgtct gatagcgaag ctggaagatg ctcaggtgca actggaaaac
     8941 aaccgtctgg agcaggagtt ggttctgctg gcacaacgaa ttgacgttgc cgaagaactg
     9001 gaccgcctcg aagcgcatgt caaagagacc tacaacattc tgaagaaaaa agaagccgtt
     9061 ggtcgtcgtc tggactttat gatgcaggag ttcaaccgcg agtcgaacac tcttgcgtcg
     9121 aaatctatca acgccgaagt gacaaactcc gccatcgagc tgaaagtgtt gattgagcag
     9181 atgcgcgagc agattcagaa tattgagtaa gcgacatatc tccgtaggcc gttgttaatt
     9241 acagcggcgc tacgcatata gtttcgtcct catatttatt tactccatac tttttctaat
     9301 gcattttact tatcacaaaa tcagaaaaat gcattcttac tacagaaaat agcaatttca
     9361 cataactaca aattatcaat ctattctctc cttaaaaaat ctcaatcgtg acaatgcgca
     9421 caaatcgcta cccttccaga cagattttta gggagagaac catgctgtta cacattttgt
     9481 atttggttgg cattactgcc gaagccatga cgggggcgct ggcggccgga cgacggcgca
     9541 tggatacatt tggcgtaatt attattgcga ccgccacggc aattggcgga gggtcagtgc
     9601 gcgatattct gctgggccac tatccgctcg gctgggtcaa acacccggaa tatgtgatta
     9661 tcgtcgctac cgctgcagta ctcaccacta tcgttgctcc tgtaatgcct tacctgcgca
     9721 aagtgtttct ggtgctcgat gcgctcggac tggtggtctt ttctatcatc ggcgcacagg
     9781 ttgcactgga tatggggcac ggcccgatta ttgccgttgt agcggcggta accaccggcg
     9841 tgtttggcgg cgttttgcgc gatatgttct gtaaacgtat tccactggta ttccagaaag
     9901 agttgtatgc cggtgtctca tttgcctccg ccgtgctgta catcgcgctg caacactatg
     9961 tttctaacca tgatgtggtg attatctcca ccctggtatt cggctttttt gcccgtttac
    10021 tggcgctacg tcttaagctg ggattaccgg tcttttacta cagccacgaa ggtcactaag
    10081 gttcaaaacc tgtgatctgc tgggcagaca gccaactgcc cagcttcttg atttgcgcat
    10141 tttccttcca ttcaataacc tgtctggcgc gccccgatcc agtccccggc agctgctgcc
    10201 agaactgttc cgtgctaaat aaaagttgcg accaggaccg ttcatcactg gcattaagcg
    10261 ccgcccgggt tagcggtatt cccattgcca tcacccagcg agtaaaaggc tgctgacgag
    10321 ccagattaaa ctgatgccat aactgcgcac ttttactttt cgcgatcccc ggcgtgttct
    10381 gtaattgctc tggcgttaat aaaagccagg aaaagatatg ctcaaagcga tgagtctgat
    10441 gcagggcgcg ccagccggcc tcaccaatgc catcaagccc cagaacctgt tttgacccca
    10501 gccagactaa gcgtgaaatg aactgttcct gacaaacatc agaagcaaag tagcaggtca
    10561 acgagttaaa gcggttttct ggtggtgtcg gttttgtacg ttctgctccg cgccagacca
    10621 catcatcaat gcgaggaatc ccctgaccgg caaggctgac gaggatctga tcaccaggcg
    10681 caatatccca ctcctgccag cgcctgacgg aaccaatatt cacccgctgg acttttttat
    10741 catccagcat gacaggtgcg agtgacgcca ccaccgatat tttaccgctc ttacccaccg
    10801 caaattgaat cgccttcact tcggcaacct gagctacagg ttgatatttc caggccacca
    10861 gccactccgc ctggcccggt agccaatgac gggattcggg ttctttcgcc gctcgcacaa
    10921 ccacgccatc ggtgacgaag ggtaattttg ctttccacca ctcattgcgt acgtgcgcaa
    10981 cctcatcagc atttttaacc gcgcgggtat acgtctgcgt taaagtaaaa cctgcggtag
    11041 ccagatcctt taaacgatcg gtcattaaat gcggtccatc cggccatgcc cagacaaaaa
    11101 cagccagtga gtttagcgta tcgctattcc cctggcgcat catcaggcca gcaacttttg
    11161 tgcgggcatt tattcccccc atttgttgtt ggatatgccc cttacgcttg agaaatattt
    11221 ccccctgaag cgtgctgttg gctaaaggcc cgctaacggt ttgcggcaca gcggaaatta
    11281 agcgaacttt ctgcgtccag tcctcgcctt tcaggccatt accgcgactg attgctttgt
    11341 tcagtttccc gtcccgataa accagggtta ccgcaacacc atcaactttt ggctgcaccc
    11401 aaagatcgct acgttcgcgc atccacagac ttaatgcgtt tttatccgcc attttgcgta
    11461 cacctgtatg ggcaaccgga tgcataaccg caccgtttaa aggcggcatc atcacatcgc
    11521 gagtctcgtt cccaaaacag cgttgccact gcgtgagacg ggcgcttaac tgatcgtaaa
    11581 caccgtcttc cacctcactt ttcccttcct tccagtagtc atcgtcccac tgttttattt
    11641 gctgttgcag gcgggaaatt tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg
    11701 cccacgccga tgattgccag cacaagatac ttattaatat cgccatccat actttcatca
    11761 tcacctccgc tgtagatagt caggcagata tacaacgtgg tgaaagcaaa gccgagtggc
    11821 aaaaacggag tctgcgagga cgcttcctga gaatcgtctt tattgcagtg aatcacaggc
    11881 aaattcggaa gcagctacgc aaaatgcaac aactttgcgc aaaaagtgtg agcaagggct
    11941 acgtcacatg gccgcgccgt gtataataag ctcgtatgta ggctttattt cgctaatcac
    12001 atacgaaaga tactcatggc tcaaggcacg ctttatattg tttctgcccc cagtggcgcg
    12061 ggtaaatcca gcctgattca ggctttatta aaaacccaac cgttgtatga cacccaggtt
    12121 tctgtttcac acaccacacg ccaaccgcgt cctggtgaag tccacggtga acattatttc
    12181 tttgttaatc atgatgaatt taaagaaatg attagtagag atgcgttcct cgaacacgca
    12241 gaagtttttg gtaattacta tggcacttcg cgtgaggcca ttgagcaagt actggcgacc
    12301 ggtgtcgatg tttttctcga tatcgactgg cagggtgcgc agcaaattcg ccagaagatg
    12361 ccgcacgcgc ggagtatctt tattttaccg ccgtctaaaa tcgaactgga ccgccgtcta
    12421 cgcggtcgcg gtcaggacag cgaagaggtc attgcaaagc gtatggcgca agctgttgca
    12481 gaaatgagcc attacgccga atatgattac ctgattgtga atgatgactt cgataccgcg
    12541 ttgaccgatt tgaagaccat tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag
    12601 cgtcatgacg ctttaatcag caaattgttg gcagactgaa cctgatttca gtatcatgcc
    12661 cagtcatttc ttcacctgtg gagcttttta agtatggcac gcgtaactgt tcaggacgct
    12721 gtagagaaaa ttggtaaccg ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag
    12781 atgcaggtag gcggaaagga tccgctggta ccggaagaaa acgataaaac cactgtaatc
    12841 gcgctgcgcg aaatcgaaga aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc
    12901 caggaacagc aagagcagga agccgctgaa ttacaagccg ttaccgctat tgctgaaggt
    12961 cgtcgttaat cacaaagcgg gtcgcccttg tatctgtttg aaagcctgaa tcagctgatt
    13021 caaaactacc tgccggaaga ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt
    13081 gatgctcacg aggggcaaac acgttcaagc ggtgaaccct atatcacgca cccggtggcg
    13141 gttgcctgca ttctggccga gatgaaactc gactatgaaa cgctgatggc ggcgctgctg
    13201 catgatgtga ttgaagatac acccgccacc taccaggaca tggagcagct ttttggtaaa
    13261 agcgtcgccg agctggtaga gggggtgtcg aaacttgata aactcaagtt ccgcgataag
    13321 aaagaggcgc aggccgaaaa ctttcgcaag atgattatgg cgatggtgca ggatatccgc
    13381 gtcattctca tcaaacttgc cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc
    13441 ccggacaaac gtcgccgcat cgcccgtgaa actctcgaaa tttacagccc gctggcgcac
    13501 cgtttaggta tccaccacat taaaaccgaa ctcgaggagc tgggttttga ggcgctgtat
    13561 cccaaccgtt atcgcgtaat caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag
    13621 atgatccaga agattctttc tgaaatcgaa gggcgtttgc aggaagcggg aataccgtgc
    13681 cgcgtcagtg gtcgcgaaaa acatctttat tcgatttact gcaaaatggt gctcaaagag
    13741 cagcgttttc actcaatcat ggacatctac gctttccgcg tgatcgtcaa tgattctgac
    13801 acctgttatc gcgtgctggg ccagatgcac agcctgtaca agccgcgtcc gggccgcgtg
    13861 aaagactata tcgccattcc aaaagcgaac ggctatcagt ctttgcacac ctcgatgatt
    13921 ggcccgcacg gcgtgccggt tgaggtccag atccgtaccg aagatatgga tcagatggcg
    13981 gagatgggtg ttgccgcgca ctgggcttat aaagagcacg gcgaaaccag cactaccgcg
    14041 caaatacgcg cccagcgctg gatgcaaagc ctgctggagc tgcaacagag tgccggtagc
    14101 tcgtttgaat ttatcgagag cgttaaatcc gatctcttcc cggatgagat ttacgttttc
    14161 acaccggaag ggcgcattgt cgaactgcct gccggtgcaa cgcccgtcga cttcgcttat
    14221 gcggtgcata ccgatatcgg ccatgcctgc gtgggcgcac gcgtcgaccg ccagccttac
    14281 ccgctgtcgc agccgcttac tagcggtcaa accgttgaaa tcattaccgc accgggtgct
    14341 cgcccgaatg ccgcgtggct gaactttgtc gtcagctcga aagcgcgcgc caaaattcgt
    14401 cagttgctga aaaacctcaa gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac
    14461 catgcgttgg gtggtagccg taagctcaat gaaatcccgc aggaaaatat tcagcgcgag
    14521 ctggaccgca tgaagctggc aacgcttgac gatctgctgg cagaaatcgg ccttggtaac
    14581 gcaatgagcg tggtggtcgc gaaaaatctg caacatgggg acgcctccat tccaccggca
    14641 acccaaagtc acggacatct gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg
    14701 aaatgctgcc gcccaattcc tggcgacccg attatcgccc acgtcagccc cggtaaaggt
    14761 ctggtgatcc accatgaatc ctgccgtaac atccgtggct accagaaaga gccagagaag
    14821 tttatggctg ttgaatggga taaagagacg gcgcaggaat tcatcaccga aatcaaggtg
    14881 gagatgttca atcatcaggg cgcgctggca aacctgacgg cggcaattaa caccacgacc
    14941 tcgaatattc aaagtttgaa tacggaagag aaagatggtc gcgtctatag cgcctttatt
    15001 cgtctgaccg cccgtgaccg tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg
    15061 ccagacgtga ttaaagtcac ccgaaaccga aattaatgtt ttatgaaccc aacacgttat
    15121 gcacgcatct gcgaaatgct cgccaggcgg cagcctgatc tgaccgtctg catggagcag
    15181 gtccacaaac ctcataacgt ttctgcgatt attcgtaccg cagatgccgt tggcgtacat
    15241 gaagttcatg ctgtctggcc tggcagccgg atgcgcacca tggcttcggc agctgcgggt
    15301 agtaacagct gggtacaggt gaaaacgcac cgcaccattg gcgatgccgt cgctcatctg
    15361 aaaggccagg gcatgcagat tctggcaacc catctttctg ataacgctgt tgatttccgc
    15421 aaaattgatt acactcgccc gacctgtatt ttgatgggac aggagaaaac gggcatcacg
    15481 caggaagcgt tggccctggc ggatcaggat atcatcattc cgatgatcgg catggtgcag
    15541 tcgctgaatg tttccgttgc ctcagccctc attctttacg aagcccagcg ccagcggcaa
    15601 aatgcgggca tgtacctgcg tgaaaacagc atgttgccgg aagccgagca gcaacgcctg
    15661 ctgtttgaag gcggctatcc ggtgctggcg aaagtcgcaa aacgcaaagg tctgccctac
    15721 ccccacgtta atcagcaagg cgagattgaa gccgatgctg actggtggtc caccatgcag
    15781 gccgcagggt aagcgcgatg aaaggtcgcc tgttagatgc tgtcccgctc agttccctaa
    15841 cgggcgttgg cgcagcgctt agtaacaagc tggcgaaaat caacctgcat accgtacagg
    15901 atttgctctt acaccttcct ctgcgctacg aagatcgcac acatctctac cccatcggtg
    15961 aactactgcc gggtgtttat gccacggtgg aaggcgaagt gctgaactgc aatatctcct
    16021 tcggcggtcg gcggatgatg acctgccaga tcagcgacgg ttccggcatt ctcactatgc
    16081 gctttttcaa cttcaatgcg gcaatgaaaa atagcctggc gacaggccgc cgcgtgctgg
    16141 cctatggcga agtaaagcgc ggcaaatatg gtgcggagat gatccacccg gaataccgcg
    16201 tacagggcga tctcagcacg ccagagttac aggaaacgct cacaccggtt tatccaacta
    16261 cggaaggcgt aaagcaggct acgctgcgta aattaaccga ccaggcgctg gatctgctcg
    16321 acacatgcgc cattgaagaa ctcctgccgc cggaactgtc acaaggaatg atgacgctac
    16381 cggaagcgtt gcgcactttg caccgcccgc ctccgacgct acagcttagc gatctcgaaa
    16441 ccgggctgca tccggcacaa cgccggctga ttctcgaaga gttgctggcg cacaacctca
    16501 gcatgttggc gttacgtgcc ggtgcacaac gttttcatgc tcagccgctg agcgccaatg
    16561 acgcgctgaa aaataaactc ctcgccgcct taccgttcaa gccaacgggc gcacaggcac
    16621 gcgtagtggc ggagatcgag cgcgatatgg cgctggatgt gccgatgatg cgtctggtgc
    16681 agggcgatgt aggttccggt aaaacgctgg tcgccgccct cgccgcgttg cgtgcggttg
    16741 cccacggcaa acaggtggca ctgatggcac caaccgaatt actcgccgag cagcacgcca
    16801 ataacttccg taactggttt gcaccgctcg gtatcgaagt aggctggctc gccggaaaac
    16861 agaaaggtaa agcacgactg gctcagcaag aagccatcgc cagcggtcag gtgcagatga
    16921 ttgttggtac gcacgccatt ttccaggaac aggtgcagtt taacggcctg gcgctggtga
    16981 ttatcgacga acagcatcgt tttggcgtgc atcagcgtct ggcgttatgg gagaaaggtc
    17041 agcagcaggg cttccatccg catcagttga tcatgaccgc cacgcccatc ccccgcacgc
    17101 tggcgatgac tgcgtatgcc gatctcgata cctcggtgat agatgagctg ccaccaggcc
    17161 gcacgccagt gactacggtc gctattcctg atacccgccg taccgatatc attgaccgcg
    17221 tgcgccacgc ctgtatgact gaagggcgtc aggcgtactg ggtttgtacg ttgattgaag
    17281 agtcggaatt actggaagcg caggcggcgg aagcgacctg ggaagagttg aaactggcgc
    17341 taccagagtt gaatgttggc ctggtacacg gacggatgaa acccgcagag aaacaggcgg
    17401 tgatgacatc gtttaaacaa ggcgagctac acctgctggt tgccactact gtcattgaag
    17461 tcggtgttga tgtacccaat gccagtctga tgattatcga aaacccggag cgtctgggtc
    17521 tggcgcagtt acaccagtta cgtggccgcg tgggtcgtgg cgcggtggcc tctcactgcg
    17581 tgctgctcta caaaacgccg ctctccaaaa ccgcgcaaat ccgcctgcag gtactacgcg
    17641 acagcaacga tggttttgtg attgcacaga aagatctgga gattcgcggc cctggcgaat
    17701 tgttaggtac acgtcagacg ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc
    17761 aggcgatgat cccggaagtt cagcgcctgg cgcgacatat tcacgaacgt tacccacaac
    17821 aggcaaaagc cctgatagaa cgctggatgc cagaaacaga acgttactcg aatgcgtaaa
    17881 aagcggcggt gagaagaccg ccgcttcatc ggttaaccgg caaaaatcgg caacatcaaa
    17941 tacagcttaa tcaccagcgc attgacgata tcgataaaga acgcaccgac catcggcacg
    18001 accaggaacg ccatgtgcga cgggccaaag cgttcagtga tcgcctgcat gttggcgatt
    18061 gctgttggcg ttgcaccgag gccaaaacca cagtgacccg cagccagcac tgccgcatcg
    18121 tagtttttgc ccatcatgcg ccaggtaacg aagatggcat acaacgccat gaagatggtc
    18181 tgtaccacca gaatcgccag catcggcaac gccagcgaag ccagctccca cagtttcagc
    18241 cccatcagcg ccatcgccag gaacaacgac aggcttacgt tacccagcac ggataccgct
    18301 cgctcaaaga cgcggtaaaa gcccatcatt gacagaccgt tgctcagaat cacgccaaca
    18361 aatagtacac agacgaaggt cggtagttca aaagcagtac cagccaaaag ttgcgcaaca
    18421 attttcccca ctgtcaggca gatagcaatc agcgcgatag tttcaatcag caccagcgag
    18481 gtgatcatgc ggcccacatc cggcttttcg aacgcagtcg gaacttcctg gtcatccgga
    18541 ataccgttcg gtgtggtgga gtgtttcacc agatagcgcg ccaccggacc gccaatcaag
    18601 ccgcccagca ccaaaccgaa cgttgcacag gccatcgcca cttctgtcgc gttggtgaag
    18661 ccataacgtt caatgaacaa tttactccat gcagcacctg taccgtgacc gccggaaagc
    18721 gtaatagaac cggccagcag ccccatcagc ggatcaagcc ctaataggct agccatacca
    18781 atgccaatgg cattttgcat caccaacagg ccaacaacca caatcaggaa aacccccacc
    18841 acacgcccac cagctcgcag actggcaagg ttggcgttca ggccaatggt ggcgaagaaa
    18901 gccagcatca acggatcgcg cagggacata tcaaagttga cttcccagcc catgcttttt
    18961 ttcagtatca gcagcgccag cgccactaat aaaccacccg caacaggttc cggtatggtg
    19021 tatttcttca aaaaggagac ggaatggacc aacttgcgcc cgagcagcaa cgtcagcgtt
    19081 gcggcaacaa gcgttgctaa agtatcgaga tgaaacatag ttactccttt gtgtccgcat
    19141 cactctttca tactcgtcat acttcatcgc gcatttgcag taaggcgaaa tatgacgagt
    19201 atcgggtcat gctttcctgg ttcgaattct tagctgaaaa tagaaaaatg gcatggattt
    19261 taagcagaaa acaactaaaa gttatataaa aaagtacatt tatctcattt aaatatttag
    19321 atgaggatgt ggcaaacgtt tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta
    19381 ccacgggatt gttttcgatg tctgtttcca ccctcgagtc agaaaatgcg caaccggttg
    19441 cgcagactca aaacagcgaa ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa
    19501 ccctgtttgc cgcctgtcag catttgctgg cgatgttcgt tgcggtgatc acgccagcgc
    19561 tattaatctg ccaggcgctg ggtttaccgg cacaagacac gcaacacatt attagtatgt
    19621 cgctgtttgc ctccggtgtg gcatcgatta ttcaaattaa ggcctggggt ccggttggct
    19681 ccgggttgtt gtctattcag ggcaccagct tcaactttgt tgctccgctg attatgggcg
    19741 gcacagcgct gaagaccggt ggtgctgatg ttcctaccat gatggcagct ttgttcggca
    19801 cgttgatgct ggcaagttgt accgagatgg tgatctcccg cgttctgcat ctggcgcgcc
    19861 gcattattac gccgctggtt tctggcgttg tggtgatgat tatcggcctg tcgctaattc
    19921 aggttgggct gacctccatt ggcggcggtt acgcagccat gagcgataac accttcggcg
    19981 caccgaaaaa tctgctgctg gcaggcgtgg tcttagcctt aattatcctg cttaaccgtc
    20041 aacgtaaccc ttacttacgc gtggcctcac tggtgattgc gatggcggcc ggatatgcgc
    20101 tggcgtggtt tatgggcatg ttgccagaaa gcaacgaacc gatgacacaa gaactgatta
    20161 tggtgccaac gccgctctat tacggtcttg gcattgaatg gagtctgctg ctgccgctga
    20221 tgctggtctt tatgatcact tcgctggaaa ccattggcga tatcacggcg acctctgacg
    20281 tttccgaaca gccagtgtcc ggtccgctgt acatgaaacg cctgaaaggc ggcgtactgg
    20341 caaacggcct gaactcgttt gtctcggcgg tatttaatac cttcccgaac tcctgcttcg
    20401 ggcagaacaa cggggtgatc cagttaaccg gtgttgccag ccgctatgtc ggttttgtcg
    20461 tcgcgctgat gttgatcgtg ctgggtctgt tcccggcagt gagcggtttt gtacaacaca
    20521 ttccagaacc ggttctgggc ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg
    20581 gtgtgcgtat cgtttctcgt gagccgctga accgccgggc gattctgatt atcgcgctgt
    20641 cgctggcggt cggtctgggc gtgtctcagc agccgctgat tttgcagttt gcccctgaat
    20701 ggctgaaaaa cctactctcc tccgggattg ccgcgggcgg tattactgcc atcgtgctga
    20761 atctgatttt cccaccagaa aaacagtaat tcactggcgg cggtaagata attaccgccg
    20821 cagtctcacc tctttcacca ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc
    20881 cttatgtgca tttcacggga tggaagacca tgaaatttat tgggaagctg cttctctaca
    20941 ttctcatcgc tctgttagtg gtgatcgctg gcctctattt tcttctgcaa actcgctggg
    21001 gagcagaaca tatcagcgca tgggtttccg agaatagcga ctatcatctg gccttcgggg
    21061 cgatggatca ccgtttttcc gcgccatctc atatcgtgct ggagaacgtc acgtttggcc
    21121 gtgatggcca gcccgcgacc ctggtggcaa aaagtgtcga cattgcgcta agcagtcggc
    21181 aactgaccga accacgccat gtcgatacca tcctgctgga aaacgggacg ctgaatctca
    21241 ccgaccagac cgcgccgctg ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt
    21301 ttaatagccc gaatagcgaa tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc
    21361 cgtggtcacc agaagccggt aaagtgctgg gtacgaaggc gcagattcag tttagtgccg
    21421 gatcgctttc gctcaatgat gttcctgcca ccaatgtact gattgaaggc agtattgata
    21481 acgatcgcgt tacgctgact aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa
    21541 acgcgcagcg taacgctgac ggcagctggc aagtggaaaa cctgcgcatg gcggatatcc
    21601 gtctacaaag cgaaaaatcg ctaaccgact tctttgcgcc attacgctct gtcccgtcgt
    21661 tgcagattgg tcgcctggaa gtcatcgacg ctcgtttgca aggtccggac tgggcggtga
    21721 ccgacctcga tctcagcttg cgcaacatga ccttcagtaa agatgactgg cagacacagg
    21781 aaggcaaact gtcgatgaac gccagcgagt ttatttatgg ctcgctgcat ttatttgatc
    21841 cgattataaa cgcagaattt tccccgcagg gcgtagcact gcgccagttc accagccgct
    21901 gggaaggggg tatggtcaga acgtcaggaa actggcaacg cgacgggaaa acgctgatcc
    21961 tcgatgatgc ggcaattgcc ggactggaat ataccttgcc aaaaaactgg caacagttgt
    22021 ggatggaaac gacacccggt tggttaaaca gcctgcaact gaagagattt agcgccagcc
    22081 gcaatctgat cattgatatc gaccctgact tcccgtggca gctcaccgcg ctcgatggtt
    22141 acggtgccaa tctgacgctg gttaccgatc ataaatgggg cgtctggagt ggctcggcga
    22201 atctgaatgc cgccgccgca acgtttaatc gtgttgatgt ccgtcgtccg tcgctggcgc
    22261 taactgccaa cagcagcacg gtgaatatca gcgaactgag tgcatttact gaaaaaggca
    22321 ttctggaagc caccgccagt gtttcacaaa cgccacaacg tcagacccat atcagcctga
    22381 atggacgcgg tgtgccggtg aatattttgc aacattgggg atggcctgaa ttaccgttga
    22441 ctggcgacgg caatattcag cttaccgcca gtggcgatat tcaggtcaat gtcccgctga
    22501 aacctacggt tagcgggcaa ttgcatgccg tgaatgccgc aaagcagcaa gtgactcaaa
    22561 ccatgaatgc gggcgtagtt tccagtggtg aagttacgtc gacggagccg gtgcagtaaa
    22621 aataaggacg ctagcggata tcagcgctag cgttccccgt tattagcgca aaattccata
    22681 ctcactgcac aataaccatt tcaccggttc atcttcttct atcgccggaa aacgtcctcc
    22741 cggctcaaga aaataattat cgtgttggtc atcatttacc atcgagattt cccagccgag
    22801 aacaaacgct ttttccgccc agaagcgatg atacagacgc ggtggcaggc aaatgccctg
    22861 ccccggcttt agcaccagcg tttcaccagg tttcatggta cgccgctgac catcaacact
    22921 cacggtaatg actcgttgat catcacagag attggcttcg ttggcccagg ccaactgcat
    22981 gcataagtct ccaccgcccc gattgaggat atcttccatt ttgtgggtgt gaaaatgcca
    23041 gggagtctgc tgatcttgtt ggatctgcaa cattttctca gcatacggtt ttggatatgc
    23101 cgccgacccc agcgcgccgt tgcgcatcgt tagcagtgtt agtcccgttt ggttgaaatc
    23161 accacgacca aaatcggtga tatcccaacc gagctgtaag tcgaccactt cttgccagtt
    23221 atcccgttcc cgctgcagcc agtcatccac cgtaaaatag gcgaactctg gcaaacaaac
    23281 gcggaaggtc tgcgcaatgg catgtgcttt atcaataaca taattaattt gtgaacgttt
    23341 cattggcttc tccgttgcgc atagcaagcc aggcagcgcc acgcattccg ctactgtcgc
    23401 cgtgctgtgc ttttacaatg ggagtggtga attgatcggt gaaaaccagt ggagcgactt
    23461 ctgcgttcag atcggtgata agcagttcca cattcgaaag cccgccgccg atcacaatca
    23521 cgccagggtc aacaacatta acgatggttg ccagcgtgcg ggcaagctgt tggcgaaaac
    23581 gtgccacctg ctgcatggca caagcatcac cacgttgtgc cagggtgaca atcgccgcag
    23641 aagtgagcaa gttaccagtc atctgttgat agcgttcact aaaaccgctg ccggaaacaa
    23701 acgactccac gcagttgtgt ttgccgcaat agcaactgac tgatggacca tcttcctgct
    23761 ccgtatagcc tggcaacgtg atatgaccgc attcggcagc atttccccag gcccctataa
    23821 acggtcgttg cttgatggca atgccaccgc cgcagcccgt accaagcgta ataccaaata
    23881 ccacgtcata atcttgcccg gcaccgtcgc aagcttctga tagcgcgaaa cagttaccat
    23941 cattggctat caccaccggt tgcccaagca attgctgcaa atcagcctgt aacgcatgac
    24001 cgttaatcac ctgaatattc gcgtttttaa tcaggccagt gagtggcgag atactgccgg
    24061 gtaaggcgat ccctgtcagc atcggtcgtt gaacgtcccg cctaatctgc tcgataagcg
    24121 ccacaacgca tgagacaaat tgttgatatg ttgacttttg cgtcgggcag cggtaacggc
    24181 gaatctccca gccatgcgca tccatgacaa cggcggcgat tttggtccca ccaatatcca
    24241 accccagata gtgcatatct cgttccttgt tgcgtaaaag gcgggtcacc ccgcccttct
    24301 ggcttagtac agtgatgctt taccgaacga accgaacagt ttcattttgt ggcgaatgac
    24361 ttcccgcgcc gcgttaatcg catccggata gatgacgttc gggtcccacc agctctcttt
    24421 agcgagaatt tcacgcactt tctggaaata ggcgtatttc atatcgctgg agatattgat
    24481 tttgccgacg ccgagcgtaa ctgattcagc aatctccgcg tccgggtttg ccgaaccacc
    24541 gtgtagcacc agcggaatat caatacgtcc ggcaatgtcg cgcaaaatgt tcatttgcag
    24601 cttcggctgc atcccttttg gatagatgcc atgcgctgta ccaatcgcca cagccagtgt
    24661 gtcggcacct gtacgagcaa taaaatcggc ggcctgcgcc ggatctgtgt aggtcacttg
    24721 tgaaacacca ccttctaccg acgttcctgt ttgtccgatg gttcccagtt caccctcaac
    24781 cgacacgcca accgcgtgag cgaggcgaac cacttctgcg gtcagcgcca cgttttcttc
    24841 atacggcagc agcgaaccgt cgatcattac tgaggtaaat ccgcagcgaa tcgcatgcaa
    24901 aacatgctct acagaggcac catgatcgag gtgcagtaca aacgggaccg gactacgctg
    24961 ggtaatttcg cgaacgtgag cgaaaaaggc atcgcccaca aaatcatgtt cgctgggatg
    25021 gatggcaata atcgccggag tatttgtcgc ttctgcttca tccacgactg cgcgaaggaa
    25081 acagttatcc gccacattaa atgcgccaat ggcaaagccg tgctggcggg taggttgcag
    25141 taattctttc atcgaaacta acatgttaaa tctccagttt cagcgtgtag tccgccctgc
    25201 cggtgcgtta ttgcactggc ataaaggcca tgacagaaaa tgaatcaggc tttgccgata
    25261 gcgccgctcg cctgtaaata cggtgtaatc gcttcttcga aggccagttc catagctttc
    25321 agcgcgtcca ccagttcaat ggatatatcg tgacagaggg tatgcaggag cgcggatttc
    25381 ccccgttcaa aaatggccgc ccccacgttg atttttgcta cccccagcgt cgtacagagc
    25441 tggagctgat cgaacggcgt accgctaccg ccatgtaatg ccagaggcac gctcgagcag
    25501 cgcgaaatct ccgccagacg ctgaaaatca atagcaggta caacgccagg cgcgtacatg
    25561 ccgtgcgccg tgcctaccga gacggccagt agatcaatgc ctgtctgcgc gataaacggt
    25621 tccacatcag tgggttgcgt catcaggtct gcattggcgg cctggtcgta acgcggcgca
    25681 tctgccagat ggcccagttc gccttccacg cagagctgcg cgctggcggc gagttccacc
    25741 accgccctgg tctggcgcac gttttcctcc aggggaaaag cagaagcatc aatcattaat
    25801 ccgctgaaac cagcacgaaa cgcctgacgt atacagtccg gatttttgcc atgatcaagc
    25861 gccagagtga ccggtactga ggcagattcc gcgagcgttt taaccactgc cgccgcgacg
    25921 ggtgcaggaa aatgcgccag atgcccctga tagagattga gaatgatcgg agcgcgattc
    25981 ttctccgcca cgcggatagc tgcgtgagca ctttccagat tcatacagtt gatcgccagc
    26041 aaggcgtaat gttcatgcca ggctttcatc accatacttt tcatatcggc aaacataggg
    26101 cctccggtta ttcgagggtg aaagtgatct cttcatcttt cagcttttga tcgctcagtt
    26161 cgtcaaactc ttcgtcgcgt tccgttaccg gtttcttcca gaatgaaagc atcacgccac
    26221 aaatcaccgt gcctataccc agcgccaggc aaaacagcag aggatgggtg aacagaggca
    26281 caacaaacat gccgccatgc ggaaccggac tctccacgcc ccacaccatg attaagccgc
    26341 ccgcgaccgc cgatgccacc acacaagatc ccactactcg cagcaggtca cgcgcggcga
    26401 tcggaatgac tccttcagtg atcatgcaga tccccatcgg aaacgcggct ttcagcgcct
    26461 cgcgctccgc acgggtatat ttatggcgtg tcagtaagaa cgaaagtgtg atgccaaacg
    26521 gagggatgat tgagccgaca aatttcacgg cttctggtcc gtagacgcca ctgaccagca
    26581 agccatcgga aaacagcgac atggttttgt tgaccggacc accgaaatcg aaggttgcca
    26641 tcgcgccaag tatggccccc atcaggaagc gcgatccacc ctgcatcgac tcgagcaaat
    26701 gaatcagtgc ttcttgtaac caggcgatgg gtttaccaat cagcgtcatc attaacagcc
    26761 caccgataat ggtgcttaat accggcaaca ccatgattgg catgagtcct tgcatcgact
    26821 gcggcaggcg gatatagcgt ttaagtagca aaatggtgta gcccaccagg aaaccaccca
    26881 acataccgcc aataaatccg gtatggattt gcccgcagag aaagccgaca atcaggcccg
    26941 gggcaaatcc gggacgatcg gcaatagaat aggcaattgc cgcgctaatc agcggcacaa
    27001 tcagccccat tccccagccg ccaatctgat taagcatcca cgggattgtt cctgtttttt
    27061 caccaacatc tgtaccgcca agaacttgcc ccaacgcaat acatatcccg gcggccacaa
    27121 taaggggtat catccacgag atgccggtta ataaatgttt tttaatttcc tgtgaaaccg
    27181 gaattttatt ttctttcata ttaagatccc tccagtataa ggatcatttg tttcccagtg
    27241 atttttcgac cttttcaaga aattgaacgg gcgatttaat gacgatctct gtctttactc
    27301 gcacaatacg tttattggtg aagcgttctt ctccttttat tttcacatct acagcaagaa
    27361 tcacgacgtc agcagcagag atatctgccg cctgtaattc attttctgta ccaatggttc
    27421 cctgggtttc aactttaatg gtatggccca gagcttttgc tcctttgatt aacttttcac
    27481 gagcaatata ggtatgagca atacccgccg tacaggcggc aacacagaca atattcatga
    27541 catacttcct gttatttagc agacatcgga ttgtgcatac tgactaaaga gatcaatgat
    27601 tttttgcgga ctcgtctcaa ctaacaattg ttctatgatg tcatcatccg ccagcgcgcc
    27661 cgcgacctgg gaaagtaaac ggatatgagt ggtattttga tcttcaagac gtaccgcaaa
    27721 taaaataata cagcgcacgc tactgccatc cagcgtttcc cagggaatat cctggcgggt
    27781 acgaccaatt gccagcgtag tattaatcac agctgaagat ttaccatgcg ggatcgcaat
    27841 atgattttca aaaccagtcg agccttccgc ttcgcgctgc cagacatctt taataaaggt
    27901 ttcccgacaa tcgatcgcac catctttttt gagaagatta gttaattctt caatcgcctc
    27961 cgctttattc gtcgcttgca tatctaaaat aacgcgattc gtattcagga tttttgttat
    28021 gtccattcgt catacctcat aaaattctga cattaaattt caacgacccg agatgtcgca
    28081 tgatgtaatt tttccgtaat caccatttca gtgacccgct taatatcggc atcattaaaa
    28141 aaagcggtaa cataagcgac cggcatagtg actgtaggca aattaatggt cgaaataatc
    28201 agttcgatat tgtcaggcaa aacctgtgat aaattcgctg ctgaaataac atcaacaatc
    28261 gtccattcag gaaatgctcg cagaatacgg cttttcagta gatgcgaagt tccgattccg
    28321 gttgaacaga ccagtaatac acgtttacgg gcaatttgcc gctccatcgc agcctgaaaa
    28381 tgaacggtca ggtaacccac ttcatcctcg ctcactgcat tctcacccca ggttttaaat
    28441 acctgattca ccacatattg cgtcaacctc cagacatccg ctaattctgc tttaatatct
    28501 tccagcaatg gattacggat atgaatgcga taatttagac ggttaatcag cggcttaata
    28561 tgcaccaata agccatcata taatgcgctg tcttcactaa aatcgcagtc cacgatgtca
    28621 ctgaataccg taattaaacg ccaggtaatc agacgcgctt cattgctggc ctgcatatgt
    28681 gaaataatgc taacatcttt ttgtccatca atcgccacac ctgatgaaat gatatattga
    28741 taaataaacc agacttcatc atcgggcaat gtatgtgcaa ttcgttgttc tatcttatga
    28801 atcatctgac tggcaacatt aaagatattt tcatcatatt gcctggtttg atcttcttct
    28861 cttgataacg cattcccgtg cgtgttgcga tacatcataa taaggatgtg agtaaaaatg
    28921 ttaacgtaat aaggttctcc caaagaccaa cttaattcat tttccatatc cagtaacaat
    28981 gactggacaa ataacacctc ttcttctcca aaataatgga ctaatgcttt atagcttccg
    29041 ggatccagac gtgaatacac gacaccttgc ggctcattat ggttaataat gccgttaatc
    29101 agtaatgcca ttgcctgtcg gacttgccct tcactacctt caatatgcgt accacttgga
    29161 ctgcggatca acgataaccc caacggcgca agccaggact caattactct caggtcatta
    29221 acaatagagg ctccgctgat aaagtagcgt tctgacaatt tactgatcga cgtttcattc
    29281 ggtgtttcac ttaataactg cgacgctatt ttaacgcgcc tggcattatt cattacacca
    29341 tcaatctctt cacattctgc caccaacaaa tgatcaagat gcagcaaatc agcaatattc
    29401 tccgcgctta acatcacacc cgcgccagca cgcttctcaa catttattct ccattgtccc
    29461 agccattgct caagtaaacg taaatcacgt tgaatggttt tttccgaaac atttaaatag
    29521 ccggcaatcg taacaatagt cgtatattcc cttcgaggta aaaggaatcg taataatctg
    29581 ttttgtcgcg aggtaatcat ttgcattttt tacccttatc acagattaag aagccccata
    29641 tggaagagct tcagttgcca tctctgaggg taaatataaa gcagaataat ttcacaacaa
    29701 tactaaaatc tgtccgtaat ccggacagtg ataaaacaaa gcaaggcggt taagggatat
    29761 ttttcagcac agatcacatt ttcgtgatga ataatcgtga tcatgctcgc aaatacgatg
    29821 gaagaaatat ttattaccag atgtgagatt atttaccgga tacgacacaa aaccattcgt
    29881 atccggcatt cttcaataga ttacaacgta attgtcagcg cattcccttg aggagtcacc
    29941 accagtccct gctcactttc agcctgcgaa ccgccttgca gaccatttac tttcacaata
    30001 ttgcgcaagc acagtgtcca gttcttcgcc tcgcccgtac cagtcacagt aatcgtgttg
    30061 ccagtacgtg ctgcttttaa agtaaagatc accgaaccgt cagcatcggg cacttcacag
    30121 acggcttcat gcccgtcttg cagattaaag aggtggaatg ccgtaccttc gtgccacgcg
    30181 taatcgggac gttgatcgtt gttgcccagc gccagtaggg tgctatcacg cacataaacg
    30241 ggcagactca ggaagctgtg ctgctgttta tgccagcgac taccgtcgag ttcatcgttg
    30301 tgccacaggt gtgtccagcg accttccggc aggtagaact gcacatcgcc cgcttcagtg
    30361 aacacaggcg caaccatcac gttgtcgcct aacatgtatt gacggtcaag gtaatcacaa
    30421 gccggatcgt ccgggaactc catcatcatg gcccgcatca tcggcgtacc ccgcgcgttt
    30481 gcacgtgcag cttcacgata cagatacggc atcatgcggc atttcagttg cgtgaagaag
    30541 cgcaccacat cacaggactc atcatcgtag gcccacggca cacgataaga tttgctgcca
    30601 tgtaaacggc tatggctgga aagcaaacca aacgcgcacc agcgtttgta aacgtgcgcc
    30661 ggagcggtat tttcaaagcc gccgatatcg tggctccaga agccaaaacc tgaaaggcca
    30721 atagacaaac caccgcgcag gctttccgcc attgattcgt agttagcgta acagtcgcca
    30781 ccccagtgta ccggaaattt ctgcgcacca acggaggccg agcgggcaaa caagacggct
    30841 tcttcctcac caacggtgtc cttgagcacg ttccacacca gttcgttgta gatgtacgca
    30901 taatggttat gcattttctg cggatcggaa ccatcaaacc actgaacatc ggttggaata
    30961 cgttcgccaa agtcggtctt aaagcaatca acgcccatcg cgaccagacc tttcagtttg
    31021 tcggcgtacc atttgcaggc atccggattg gtaaagtcat aaatcgccag acctggctgc
    31081 catttatccc actgccacag cgaaccgtcc gggcgtttga gtaaataacc tttctcttgt
    31141 aactctttaa agacggggga tttttgccca atataggggt taatccagac gcagattttc
    31201 agccctttcg ctttcagacg gcggatcatc ccttccgggt ccggaaaagt cagcgggtcc
    31261 cactcaaaat cgcaccactg gaaggctttc atccagaaac agtcaaagtg gaaaacatgc
    31321 agcggcagat tgcgttccgc cataccatcg ataaagctgt ttaccgtcgc ttcgtcgtag
    31381 ttggtggtaa atgaagtggt tagccacaga ccgaaggacc acgcgggcgg cagcgccgga
    31441 cggccagtaa aacgggtata acgatcaagt accgctttag gcgtcgggcc gtcgataaca
    31501 aagtattcga gatattcact ctcaacgctg aactgtactt tggagacttt ctccgatccc
    31561 acttcaaaag agacgcattg gggatgattg accagtaccc cataaccacg gttagtcaca
    31621 taaaacggga tatttttata cgcctgttca gtacttgtgc cgccgtcccg gttccaggtt
    31681 tctaccgtct gaccattgcg caccagggca gtaaagcgct ctcccaggcc gtaaactgtt
    31741 tcgccaacgc caagatcaag ccgttcaaac atataatttc gttgattatt cgtgtcctgc
    31801 acgtagccat tatttttcac ctgactaccg gtaatacgtt caccgttgcg cagaaaatcc
    31861 agtgaccaga actcaccttt gctgacacgc gcacttaagt tgccactttt aaactcagcg
    31921 taacgttctg tgttttcgat agtgaccttc acgtcctgca aaatattgag cgggtaatga
    31981 ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgta caccgacaat accttcctgt
    32041 ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct gccaggtacg ttcacgcaca
    32101 tcacgagggg cagcatagac caccatttca ttaccctgct gttcaacctc gaacacctga
    32161 agcgggtgaa tcaaattgag gtcaggttga atcaaccagt ttccgtcgct aattttcatt
    32221 gccgttcctt agttctgcac ttcttgagag gtgaattgtt gctggcaacg gcgtttgccc
    32281 tgcgccagct gttccataac ggttttcaga ttgtgcgtcg tgagcgagta gtagcgttta
    32341 gcgataatcg cgctcagcaa gtaacagatc gccggaacaa tcgtgaatag cgcgataatg
    32401 atgctaattg tgacgctgtt ctgcgctttt tctgccgcat catatccgcc ataagccagc
    32461 atccagccga taagggcacc accgaaggcc aaccccagtt tgagcacaaa cagcgtgcca
    32521 gcaaaactga tcccggtcag gcgtttacca ttgcaccact cgccgtagtc gacggtatcg
    32581 gacatcatta cccactggat aggtgtcacc agttgatgca acacaccaat cacgaagatg
    32641 aagacaaaca tggtgatgct ggcctgcata ggaacaaaga acatcgcgag gctaatcact
    32701 gccagcaggg cgttcgtcca ccagaagata gtgactttac atttccagtc ggtcagcggt
    32761 ttcgccagtg cggaaccaat caggttaccc acgcaataag tggtgagaaa agcaacaaac
    32821 acttccggcg tacccaaaat ccatgtgacg taatacatca tcgccccacc gcgtacgcac
    32881 accgccagga tattgaaaat tgtcaggaaa ccgacaatac gccactggtc gttttgccag
    32941 atatcacgta aatcatcccg catagacgtc gttgtaggtg gtgcttcaac gcgttcttta
    33001 gtggtgaaga aacagaatgc cagcatcata aatgccacca cggaaaggac cgcgataccg
    33061 ccctggaaac cgagtggttt attatcacca ccaattaaat taaccagtgg catcatcaga
    33121 acagtagaga gcatgcctcc cgcggtcgcc agcacaaaac gccaggattg cagcgagata
    33181 cgctgagtcg ggtcattggt gattacacca cccaatgcgc agtaagggat attgacgatg
    33241 gtataaagta aggtaagtag ggtataagta attgctgcat aaatcatttt tccgttcata
    33301 ctgagatctg gcgtgctata ggccagtaca cagacgatcc cgaatggcag tgcgccaaac
    33361 agcacccacg gccgaaattt accccaacga gagcgcgttc gatcggccaa caaccccatg
    33421 caaggatcgg aaatcgcatc cagtgcacga gcgaccaaaa acatggttcc gacaaatccg
    33481 gcaggaatgc caaaaatatc ggtataaaag aacatcatat ataacattac gttatcgaaa
    33541 ataatgtggc tggcggcgtc tcccatgcca taaccaattt tctctttaac ggacaacact
    33601 tcactcttca tctgcttttc cttgatgcta taggggtgct gagaccgata acgcattttg
    33661 taaaccatcc ctgcgagagc gaatattccg atatctggtt attaatttat gattcttgtt
    33721 ttatgtgatc gtggtagcgt taatttcgct aatatatcat tgtaaaatat gagttttata
    33781 tgaactataa tgctttcgtg ataatacgct gcgtgtatta ggcggaaaaa actgatctgg
    33841 gggatgtaga aactcaagga agtagctata atgcgccccg cctccatgta gcaatcgagg
    33901 cgcggaagat cgtcgtctcc ggtgaggcgg ctggacttca aatccagttg gggccgccag
    33961 cggtcccggg caggttcgac tcctgtgatc ttccgccaat taacatattc tgaactctcc
    34021 cgatgtcaac ccccccttct aatcccttgt atatcaggat tcctttgtat ccagaagtca
    34081 accgacttca ccccgtatca agttgctttt gggggtactt tagggggtat tatttcctat
    34141 tgaaatatca gcacccccat aattgttaaa atatgatata taacaacggc tttcaacaac
    34201 atctttgtta tacaaggggt gcattggggg tatcaggtta attactggca gaaatccccc
    34261 tcaggaacat gaattcatta aaagccggag gatgtcatgg cattaaccga tattaaagtg
    34321 aaaaccgcaa aaccaaaaga aaagccctat aaacttgctg atggcggtgg tatgtatcta
    34381 ctgattaaca ctaatggttc taaatactgg cgtatgaagt accgctttgc tggtaaagaa
    34441 aagatgcttt ctattggcgt ataccccgat gtaacgctag ccgacgctcg tgaaaaacgc
    34501 agcgaagcca gaaaaatcct cgctgctgga ggcgatccag gggtggcaaa aaaagaagaa
    34561 aaaatcgcgc agcaaatgag tttgaaaaat acgttcgagg ccattgctcg cgaatggcat
    34621 cagtcaaaag ccgatcgatg gtcattacgc tatcgtgatg aaatcattga tacctttgaa
    34681 aaagacattt ttccctacat tggcaaacgc cctatcgccg aaatcaaacc aatggagtta
    34741 ctggaagcac tgcgtaaaat ggaaaaacgt ggagctctgg agaaaatgcg caaagtccgc
    34801 cagcgctgtg gtgaggtgtt ccgctatgca attgttactg gtcgggcaga ctacaaccct
    34861 gctcccgatc ttgcctgtgc tttagccaca ccgaaaaaag tacattttcc ctttcttact
    34921 gctaatgaac ttccccactt cctcaacgat ctggcgggtt ataccggaag tatcatcact
    34981 aagacagcta ctcagatcat tatgttaacc ggtgtacgaa cacaagaatt acgtttcgca
    35041 cgctgggagg atatcgattt tgagacaaaa ttatgggaga ttccggcaga agtaatgaag
    35101 atgaagcgac ctcatattgt gccactctct gagcaggtca ttgccctatt taagcaactt
    35161 gaacccatca caaaacatca tcctctggtc tttatcggta ggaacgatcc tcgcaagcca
    35221 attagtaagg aaagcattaa ccaagtcatt gaattactgg gatataaggg aagactcaca
    35281 gggcacggtt ttaggcacac catgagtacc attctgcacg aacagggctt taattctgcc
    35341 tggattgaaa tgcagttagc tcatgtggat aaaaacgcca tcaggggtac ctataatcat
    35401 gcccagtatc tcgatggtcg ccgtgaaatg atgcagtggt acgcagatta tattgattcg
    35461 ctttctaagc aagagagtca gggttaagag cacttgtcct gatattttct ctcaatccac
    35521 aaattctcct ccggcctcca gatccggagg aattttgttt ttatgcttta tttttagata
    35581 ttttttgttt tttgacaaaa tatttaatat tgtatctgta aacgatctca aaacatcaca
    35641 ccgttatgga ccgctaacaa ttttgatacc tgtaatatca atcatcatca tgtcgttatt
    35701 tcagcaccat tccccccaaa aaaaaatcga ttaatatgct gtttcagctg ataattcaca
    35761 atacatcgga atgcagataa agataaattt tcattctcat gtaaaaacac tgtcgatgat
    35821 attataaaca acattcatcc cggacaaaaa caagacttga tacacaacat catgactttt
    35881 atgcttttca gattgtgtcc catccgtttt acaggctcag aaaatgaacg acagattacg
    35941 ctttgaagtg aacaataacc agggtcgttt cgtatttccg gatacctggt ttggcccatt
    36001 actgggggag tttgaagaag tgcttgatgc atacgacact gatgaaatca gcgagacaag
    36061 ttacatcaac aaactcagac gccttgctca acaggagcct gactttattg atatacacgc
    36121 tcatctggca tatgcatttc tggagcagaa tgccccgcgt aaggcactga acgccgctct
    36181 taaaggactg gcagcaggta atcgtctcat cccggaaagt ttcaatggtg aaatcatctg
    36241 gatgcatccc gaaaaccggc cttatctgcg cgcactttat gcagcaattc tcgcaaatgt
    36301 gcacttacag cgtcatcagg atgcggttat gcttactgat aaaatactgg catacaatcc
    36361 tgaagataat cagggtgccc gctggttgtt gggctcagaa ctgttacgaa caggcgatca
    36421 taagcaagcc ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga
    36481 acttggtctt ctgcattttc tgaatgggga acatgttaag gctgccacag cttttcgtca
    36541 tggctttgct acaaacactt acatcgcgga aatgctgtgc ggaaatttgc atccatttcc
    36601 acttgctgtg cggcataact tctccggctc tctggatacg gcagaagatt actatgcaac
    36661 atactcacca ttatggggac aatatccaga agcattgctg tttgtaaact ggctctataa
    36721 ccattcctct gtgctgcatg aacgagcaga aatcattaaa tgcgccgaaa tgctgatgca
    36781 ggaagatgat ttcgaaatat gcgaaagcat tttaagacag cagaagctgt tgcgggaaag
    36841 aattgatgag acactttctg aggaaattgt acagaaatgc agaaatatta acggtgaata
    36901 tgtctggccc gggatattgc cgttttcagc agcaggaatg aaacactcaa gcatccagca
    36961 tcagtagata ttgcattagt gtatctttca aacaagtaat aatttatcca ccagtaataa
    37021 cacagccccc cttccccagg ttatcgccct gggggcattt tctgaaagtt tcctttaaaa
    37081 aagaatagag tcgtaaatta ctttaacgaa ttattccagg agttcattaa agttctttcg
    37141 taatactttg tagtcaaaca cgttatgact ggcatcaaaa accacggatt tcccctgcca
    37201 gcgggtaatc acctcctccg gtgttttcgg ttgagccggt ttggttacgc ccatccatgc
    37261 attaatcaga taatcattat acgcagtgga aaaaacgctg ttcaccgtct ggcgatcgac
    37321 atgctctccc agtgccgggc tgtaccagat aaacatcgga acatgataag cctgctggct
    37381 gggttttatt ccgccatgaa aataggcatg ctcttttgtc gggtcatact ccaggccatg
    37441 atctgaaaaa tacatgacgg atgcccgacg cgtttccagc atggtgaaaa cctgtcccaa
    37501 caaactgtcg gtataatgaa tggaattatc gtaacagact tcttctgtat ccagcggttt
    37561 aaacacggct ttatctctgg gccagttact gcagactggc tcatgactgc ccgtcagatg
    37621 cagaacaatc agtttcctgc tccccggatt actgttcagt gcttcagcca ggtgtgggag
    37681 cagtaactca tcatagcctc tgacataaat tctgtttctg gcccgcatgg cgatactggc
    37741 cacagccgtt ccgttctgtc ggaaagctga ctgcgcactg agccaccagg tatcgaaccc
    37801 cgcctgattc gccatgttga taatattatc ggggtaatgg cgaacatcat gatgcagcac
    37861 ggtatcagca gataatgcca gcggtaccgc aagggcggta taaggtgccc cgctgattgc
    37921 ctgagtgaac agtttcagtc gtgatttctg tttctgaagc tctggagtgg ttgggcgcga
    37981 atacccgtaa atggacatgt tatcagtcct tgcagattca ccaatcacca gaacaaatac
    38041 gtcagtgttg ttatccgtaa tcaccagatc gtagtgaggt atggtatcag caatcgtcat
    38101 taatctctga tgctcctttg cggccagtgc aaaatagttc agattaaaga aaggggtata
    38161 tgtggcaaac cgggaagcga ctatgtaagg atcaacttca tttatttgat actgtttctt
    38221 caacgcaaac tgaaaggaag aatataacgt tactgtaatt aacaataaaa atgtaattac
    38281 cgtgaccttt tccgatggca atgaggacat tttatttatt gctgaacaga agaacaataa
    38341 aaagaggata ataaatgcga taacataaac aacatacatc ctgaacattc ttattacttc
    38401 atcaggatct gtctgaagga tactgattgc aaaaccatca ttaaatgttg taccgaatgt
    38461 acaccatgaa tacaaactga tactgatatc tgccgctgtc agtaatgtga acgaaaaggc
    38521 aagaataaaa cgcagcgtaa gcgatttcat cgccttaata ataagaagcg ccagggtgaa
    38581 gaataatatc cggtaaatca gatgcgctcc tgaggataca aggatattag aaagggtaaa
    38641 gacaaccacc gataacacga ggcgcttatc attgctaatc cgtgaccagt gtatgaacgg
    38701 aagattaatt ttcataataa ttgagtaaaa acgatatttc tgcccggcgc tgaccgtgaa
    38761 acataccgtg gcagatgaat acatttttct gaataatcat aaaacgggca tcaaccgccg
    38821 ccacaaatgt agcggcgtaa acaggatcgt catgatgttt tctttcgtga cagaagagaa
    38881 cggcacatac cgcccgcaat cattatcagc aggaataaca tccccaggac aacaattact
    38941 cttaatgccg gatgaacccg atcggcgaga aaaaataact ccatcccgaa accaataaca
    39001 agccaggcca gcgccattcc gacgagccct tttaacagca atctcacgct cctctccttc
    39061 tgcattctta acgaaaatgc cttacaataa cagcagccgg ggaaggcaac gctggctgct
    39121 gaacggtatg acattttact gctttatgtt ctctcagttt ctgtactgac gccgccatta
    39181 atggtattac agtttattca gaataaggca ggcggcctgg tttaatgcaa taaactcctg
    39241 ccagctgtac tgagactgat aattcagtaa atactggtct tcaacccggc aaatctgtgg
    39301 gcgtttatca taaattgcac acagatgaga atcagtctga tagtgacggc aaataccgtc
    39361 accacgatcc agacattgcg tttcaccagc cctgttgaca tgacgacaac aggccccaca
    39421 ctggttacag ggaaatggag ggtgtaattt catcgcttat ttaaccagtc acgaacagaa
    39481 gccacatttc ctgactcaaa cggcaactca atccctcgtc ggctgacttc ttctctcagc
    39541 agaagtccgc gctctatctc actgtttacg ctatccagat gaagcgaaaa gtgatttaac
    39601 gcggcagcat caacagaaaa ttccagcgca gtaagttcta caagatagcg atcaagttca
    39661 gcattgactg cctgaaagta ttccagtgac cgccggaact tatccttaaa tttgtccaga
    39721 aatgcccaca cccgctgcat aacctcgctg tattcgagat aataattcat caccagcccg
    39781 agaatacctg tcgtcagcgc accacaaaag gcagcaagct ctgaaccaaa tggaaaaata
    39841 aggatctgcg ccatcgcctc atttatcatc acccctccgg caacagccac acctgctgct
    39901 atcagtttag tgaccgcttt ggctaactgc cccggcgata atccctccgg gttaaatacc
    39961 aggactttaa aggcctgaac gagattgttc cacatttccc tgataagccg gacaatcatt
    40021 ctttccgtcg tcaggaatat atttaacagc gttgtggtaa tacctgacag tatccctccg
    40081 atgacaccat ctttaaaggc aattaaaaaa gaactgaatt tttcctgtac tcgttcctgg
    40141 caggcacgta atgctgacgt tatttcggac aggcatttgc cggcgctgaa tgagttccgt
    40201 tgctgcctga taatatgcgg gattcgttcc ctcagttcga accagatctc ggcaagaata
    40261 agccccagca tctgtcgcgt ccccatacga aaaccggaat taagtgaagc actggcgaca
    40321 gcagtagcaa acttactact caggtaatag ctctgattaa tcgtggcctc atatttttcc
    40381 cgggcatatt tatcagccct ttccatacgc tcagtatctg catcagcttt ttttctcaga
    40441 tttatcagac gtttcttttc cttttctgtc agcgttgcct gagattcaag ctctgctatc
    40501 tgccttaacg tatcttctct gtttttctgc agcgtgcaaa taaacgcatc cattgagtct
    40561 gctttctttg atttattgag cgactcatga gtgaatgcca gatttgaagc gtcatttgcc
    40621 agttctgcac catcccgttc agcaagaatt ctccccggat catcatgaat ttctttagcg
    40681 gaaataatat gatccagatt ttttttgtcg tcagaagcaa acacctcgcc tgtatatgta
    40741 tcagtaagag tccctgcctg atgcgcacgc tttccttccc ggttcgtcgc gatatagttc
    40801 tcatggctgt ggtaatgatg agaatcatat tcgccacgac cttcataccg ctgtcgctca
    40861 gcctccgtgg cataaatgcc attacgcaca ttatggatag tgtcaacatc accaccctta
    40921 cgatcattta gcaacagaaa atccagcccg aatgatgtaa ccaggctctt caccatggtc
    40981 tgatgtaact cttctgtcca gtcaaatttt tccacattca tgttatttca cttcttgtaa
    41041 acccgggctt taacgcccgg taaatcatta actccgggat gaaaaatcat caaatttcac
    41101 aaccgaactg gccagtacat catccagctc atccttatta ataacgttca ttccattatc
    41161 atccgtttcc atttcaataa cgttatcttt tatcactgcc tcaccatttt cctgtttccg
    41221 cggtttaaac agtggcgttg taataatatc cgtcataata gcggccagtg aatatccatg
    41281 gtcaataagc acaatgactt catctgatac actgatatcc tgttgttctg taataacaga
    41341 atgcatggat ttaagcggct caaaatagtg tttaataaat acagcatgca ttctgttcag
    41401 tgcagcgaga atttcatcaa cgtattgatt aacttttgca aaatgctccc gggccagatt
    41461 cattttctta actgcccgtg caacatcgca ggcacactga tgggcatttt caagagcttt
    41521 cgtcgcatgt ctgtcgtaag cccagcccgc aatggcaatc aacggtgcag ccactgcagt
    41581 gcctaatacc attgcgccac cagccattcc catgccccct gcagccagtg agcccccacc
    41641 aattgccgcc atcgtcgcgt tataagccgc ggcaccagaa agcgctgcaa tgggtgtacc
    41701 tgtagaagcc gcagcaaatg ccatcactcc gctatacaca gcaaaacccg cagcagcccc
    41761 ggaaactccg gcagcgacga ccgtactgag atattctgtc gctgaaatgg ccaggttctg
    41821 aatcttattg atgttatgtt gcgggacact cagtttcagg tctttattgc cctctttttc
    41881 cagacgcgcc aggagctcag cagcaatcct gtcgaactca ctgaaatctt taccaatctc
    41941 aagctcttta tttccgagcg ttgttaattt tgatgatgtc tgagcttcag cctgctcaaa
    42001 tttttcttta tgctcctcgt aacgctcttt tgctttttcg aggatatcct ctgcttctga
    42061 ctttttttga tatccatcgt aagctttttt tccgccgaat gccgcagtgc cggcagccac
    42121 acccaaaata atgaaaggta acgccattct tttcctcctg tacaggttgc tttatcatca
    42181 gtcaactgaa atatttcagg aaacattctc agcggtgagg tattttccta acattgttaa
    42241 cacggcttct ttcagccata ctggttcaat aatttcaaca ttcggtagcc agtataaaat
    42301 caatgggata atttgatttt tatgtgcagc cttgcatgac agcataatgc cattgtcatg
    42361 tttgtgtagc aattgctggt aaggaagtaa gtcccgtcgc aaaaaataat gggcgatatt
    42421 actgtgaatg aacaacacaa catcaaatgt tttgtcgctc acccacgggt cacatgactc
    42481 acttactagt gactgtattt catcactctt tgcaaacttt tcttttttta tatccagcca
    42541 ttttatttta gctaatgaaa acgactttaa cctgttctct tcagtagcca gcaaatacca
    42601 tatacttctc tgattagtta atttataggg atgaacgatt ctggtcttac cggcatatgc
    42661 aatctggcaa acattattgt tgcagatcgc tttttcaaga acatcaagat attttcttaa
    42721 atcatccctg atagtgtgct cagggttatg aaactgaaca cgaatatgct tttcggttgc
    42781 cctgttttct aatttctgcc agaaaccttc atcccgttca ggaaaaatcg catcagtatc
    42841 cagtaacctg gccagagatt tgtgcagtga ttctttgctc tgatttcctg ctcttgctga
    42901 aataagccgg tattttccat cgccacaggc ttcaataaag ggagaaagca tattcagatc
    42961 acgatatgct gttcgctcag tgatgttgta ttttttcatc aaattatggc gactgaccac
    43021 gccataattc tgtaattcaa caaggatatc aatcaaacgc tccgctgagc gatttctcgt
    43081 tgagtcagcc ataaatcact gcacctgtag tattactctt gtgtaagtga attatataaa
    43141 tacccctgac atcatttgtc agttccacaa aactacgccc tccggtaaca gatttttatt
    43201 tgattacaat caaactttaa accagatgtc cgggtgtttc tttcgtaata aggtttattg
    43261 tttccggaag ggctgatata gaaaaaataa catatcccaa caacagtctt tttttcattt
    43321 aacaggctga caaccatact gcgtatcacc tgcacctctg taaaaaatca acgcgggtaa
    43381 tatttttgct ctgtttatat ttcatatatc tgaaattatt agttaccaga gggagaatat
    43441 catcgggata tctgttgttg acaatttgtc atacctgcac tttactgtgc cccaaagcac
    43501 aggcagaaat ggctgtcaga agttatacgt tgcgcctaac ataaactcgt tacttctgac
    43561 atcaacagag gatttatatt tagcaccatc ctcatcttta taagtcagag tggaatcccc
    43621 tgcatccaga taccggtagc tgaggtcaag gctgaaatcc tgcgtcacat cgtatttaac
    43681 accgacacca aggctccatg ccaggttgtt ttcatatttt gacgctgaag ttgaatatac
    43741 ttcactgcct gccgggctgt tatcggtata tatgtacgaa gttttatgat gaacccgtgc
    43801 ataacccagg ccagcagata tccatggcgt gaatgcactg ctgtttctga aatcataata
    43861 cgcattcagc atcagcgtgt ttacactgag cttgttctgt gcgtcatcaa actcggcgcc
    43921 accgacactt tcccaataac tcagacgata ttttgattca gcgttaccgc ggccataaaa
    43981 ctccacttca gtacgcaccg gcacattgta gtgctgataa aaatcgtatc ccaccgcaag
    44041 gcctgtgcca aaaaccgtat cacttttgtc accgccttta tatttatctg cccatgcccc
    44101 ttcaccatca acgaaacgtt ggtctgaaag tgacataaca gaagcaccgg ctttaccggt
    44161 cacataaaag cctgttttgc tctcatcagc cagagtctgg gccgaaaaca tgcctgccat
    44221 tgtaagcgct gaaaccgcaa taaccttttt catttcaatc atctccgttg gttcttaccc
    44281 tacccgacaa agagggttgc ctattttaaa accatgcaaa agacagggta aaatggttac
    44341 aaccgatcga tgtgatcata tcgatcggta aaatcgatcg atattggtga gggttttcgt
    44401 ttcgaatcaa caaaatggta atgatggagc ccggactgaa atgctgtgtc ccagaagcct
    44461 gggaggtttg cattacaact gcactacagt acaaaatgtc agacacagta aaaaagactg
    44521 ggtaatcaac ccaccagaga aaacacgttt attgcaaaag agaggggaat tatgtgcaaa
    44581 ctgactagag aagaagaata cagcatcatc agtcggacaa tgacaaatat tcgttcacta
    44641 cgcatgtatg tacgcggagt tgattttgag caattactcg acatgcagga aaggctcaac
    44701 gcggttattg aagaacgccg taaagatgct gaacgtgaag cggctgaacg agccgagcgt
    44761 gaacagagac gtcaggaact gcttcagtta atcgccggag agggtttttc actggaagaa
    44821 ctgcttgatc ggcctgaaga tacgccaaaa tcacgtaaaa agacgttgcc aaaagcaccg
    44881 cccaagtatc aatttgaaga aaatggtgaa actaaatact ggtctgggcg tggacgtgcg
    44941 ccaaaaccga ttgatgaagc aatgaaagcc gggcgttctc tggaagattt tcgtatcaat
    45001 aaaaattcga acgacataac agatgaacag taatatggca aaagcaccat tttgttaagg
    45061 gagaaggcta tgagtaatac atcctacaaa caaattatcc ctgtgtcaga ttggtatttt
    45121 cgtcacgaaa atgtctctgg tgtggcagaa aagtcaacac tataccaact ggctacatgg
    45181 gcacttaaat aaaatggtga aatagttggt ctggtgacag ttcgtgatga taatgggcat
    45241 cctaaactgg ttacccctcc ccctgttcct ggtgattatt tgcataaaga acaactcacc
    45301 aatgatgaaa aagagtgggc gaagagacga taaactggtt ctccgccagt tgcgacagtc
    45361 acatcatcgt cgtccgttac cggcgcatct tccccgtgaa gtaaaacgtc tggagccgac
    45421 agaaagcttt tacccagaat gtagaggtga gcgggattat ctgagtgaag tcagcgcaga
    45481 acagctggaa ggcctccgtc tcttcaccaa gactcagttg ctggtctttc tgacggaagg
    45541 cttcagcctg ctcagagagc aacctttcca gttctgcaat gcgaataagg tatttctgac
    45601 tcacggccgt tttataatgc ggccatgata tttttacaac attgtcagtg cgttaaggcg
    45661 ggatgttttt ggctgacgcc agtccagctt atcgaggagc attgccagct gcgagcgggt
    45721 aatggatact ttaccaccac gcaccgcagg ccagataaac tgaccttctt ccaggcgttt
    45781 ggtgaacagg cacaggccat cagcatcagc tcagaggatt ttacctacgt taccaatact
    45841 agcttaatgg ctacatacgg cggtcagttt acgcttacct tttccccacc aataggatat
    45901 ttttatggga atgataatta tcattggcgg aattgttctt ttgctgcaat tatgttggtc
    45961 ggtcttctgt gcaaaagcat cagcaccagc aaaaattcgg ttgctcaatt ttgcatggtt
    46021 cttttgttgg ctcccctgct aggggttgcg ggatatatta tctataccgg gattgagctt
    46081 ttttctaatg caccgtaatc ctcgccataa acaatgaatt accgccttaa cctagcttta
    46141 tcctccccct taagtggcaa aaacaggact tttagcccct gttttatatc gggtcgggac
    46201 gtatttacct gcctatatag ccatgcctca ataagacagt ataactttcc gggagagagg
    46261 cgctgagcaa cgtgggagct ttaacgcggg ttaaatgcag tacccaaccc gcgaatatca
    46321 acgtgattca ccgccggatt tactgctacg ccgccctcag ggtattgacg ttgaacaaaa
    46381 aacctaaaaa accaataatt ccagttgaca cggaaagtca tatcagcgtc aatacgccct
    46441 cggttaagcg taccttaaac ataggcctat atcttattgg tgaagtatct ggttttaatg
    46501 ggggctacta caaatgaaca cccctcactt tttactgtac atatgaacag tattgagcga
    46561 taatacatca tatgtgaaag ttagaattga agaaatgaaa gaaattgaca tcaaattgaa
    46621 aattgtagag tttcttttaa actctgaacc cgctgataca tacctagctg ctgaggttag
    46681 atttagtttc ggatcaagac gtgctgatat cgtttcagtt tcatcggata ttgcaactgt
    46741 ttatgaaata aaaagtgaaa aagattccgt tgaaagactt gtttatcaaa tagatagtta
    46801 taaagaatat tttgattact gttatatagt ctgtgtggat aaaaatttag atgcggtcag
    46861 aaagaaaatt agcaccaatg tgggtattat tgtcgtggat gatattaacg ttaagagaat
    46921 tagaaaggca aagcgtattg cccgacaaga aaaattatgt ttagcgagca ctataccagt
    46981 gaatgaattg a
//
LOCUS       DAZKHK010000020        45334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKHK010000020 DAZKHK010000000
VERSION     DAZKHK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.020"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(271..816)
                     /gene="orn"
                     /locus_tag="ACHOS5_001186"
     CDS             complement(271..816)
                     /gene="orn"
                     /locus_tag="ACHOS5_001186"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP9776036.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            911..1963
                     /gene="rsgA"
                     /locus_tag="ACHOS5_001187"
     CDS             911..1963
                     /gene="rsgA"
                     /locus_tag="ACHOS5_001187"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP9776037.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2060..3028
                     /gene="asd"
                     /locus_tag="ACHOS5_001188"
     CDS             2060..3028
                     /gene="asd"
                     /locus_tag="ACHOS5_001188"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP9776038.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3050..6373
                     /gene="mscM"
                     /locus_tag="ACHOS5_001189"
     CDS             3050..6373
                     /gene="mscM"
                     /locus_tag="ACHOS5_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9776039.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6512..8014)
                     /gene="yjeM"
                     /locus_tag="ACHOS5_001190"
     CDS             complement(6512..8014)
                     /gene="yjeM"
                     /locus_tag="ACHOS5_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9776040.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNTMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8233..9210)
                     /gene="epmA"
                     /locus_tag="ACHOS5_001191"
     CDS             complement(8233..9210)
                     /gene="epmA"
                     /locus_tag="ACHOS5_001191"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9776041.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9535..11343
                     /gene="frdA"
                     /locus_tag="ACHOS5_001192"
     CDS             9535..11343
                     /gene="frdA"
                     /locus_tag="ACHOS5_001192"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9776042.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11336..12070
                     /gene="frdB"
                     /locus_tag="ACHOS5_001193"
     CDS             11336..12070
                     /gene="frdB"
                     /locus_tag="ACHOS5_001193"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9776043.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12081..12476
                     /gene="frdC"
                     /locus_tag="ACHOS5_001194"
     CDS             12081..12476
                     /gene="frdC"
                     /locus_tag="ACHOS5_001194"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9776044.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12487..12846
                     /gene="frdD"
                     /locus_tag="ACHOS5_001195"
     CDS             12487..12846
                     /gene="frdD"
                     /locus_tag="ACHOS5_001195"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9776045.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12909..14042
                     /gene="ampC"
                     /locus_tag="ACHOS5_001196"
     CDS             12909..14042
                     /gene="ampC"
                     /locus_tag="ACHOS5_001196"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9776046.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPVWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14131..14664
                     /gene="blc"
                     /locus_tag="ACHOS5_001197"
     CDS             14131..14664
                     /gene="blc"
                     /locus_tag="ACHOS5_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9776047.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14661..14978)
                     /gene="sugE"
                     /locus_tag="ACHOS5_001198"
     CDS             complement(14661..14978)
                     /gene="sugE"
                     /locus_tag="ACHOS5_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9776048.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15161..15307)
                     /gene="ecnB"
                     /locus_tag="ACHOS5_001199"
     CDS             complement(15161..15307)
                     /gene="ecnB"
                     /locus_tag="ACHOS5_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9776049.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15418..15543)
                     /gene="ecnA"
                     /locus_tag="ACHOS5_001200"
     CDS             complement(15418..15543)
                     /gene="ecnA"
                     /locus_tag="ACHOS5_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9776050.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15595..16161)
                     /gene="efp"
                     /locus_tag="ACHOS5_001201"
     CDS             complement(15595..16161)
                     /gene="efp"
                     /locus_tag="ACHOS5_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9776051.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16203..17231
                     /gene="epmB"
                     /locus_tag="ACHOS5_001202"
     CDS             16203..17231
                     /gene="epmB"
                     /locus_tag="ACHOS5_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9776052.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLHQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17466..18335
                     /gene="yjeJ"
                     /locus_tag="ACHOS5_001203"
     CDS             17466..18335
                     /gene="yjeJ"
                     /locus_tag="ACHOS5_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9776053.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18385..18738)
                     /gene="yjeI"
                     /locus_tag="ACHOS5_001204"
     CDS             complement(18385..18738)
                     /gene="yjeI"
                     /locus_tag="ACHOS5_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9776054.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(18876..20522)
                     /gene="groL"
                     /locus_tag="ACHOS5_001205"
     CDS             complement(18876..20522)
                     /gene="groL"
                     /locus_tag="ACHOS5_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9776055.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20566..20859)
                     /gene="groS"
                     /locus_tag="ACHOS5_001206"
     CDS             complement(20566..20859)
                     /gene="groS"
                     /locus_tag="ACHOS5_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9776056.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21135..22391
                     /gene="yjeH"
                     /locus_tag="ACHOS5_001207"
     CDS             21135..22391
                     /gene="yjeH"
                     /locus_tag="ACHOS5_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9776057.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGLAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(22407..22883)
                     /gene="fxsA"
                     /locus_tag="ACHOS5_001208"
     CDS             complement(22407..22883)
                     /gene="fxsA"
                     /locus_tag="ACHOS5_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9776058.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGEDPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            23220..24656
                     /gene="aspA"
                     /locus_tag="ACHOS5_001209"
     CDS             23220..24656
                     /gene="aspA"
                     /locus_tag="ACHOS5_001209"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9776059.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            24774..26075
                     /gene="dcuA"
                     /locus_tag="ACHOS5_001210"
     CDS             24774..26075
                     /gene="dcuA"
                     /locus_tag="ACHOS5_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9776060.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26191..26529
                     /gene="cutA"
                     /locus_tag="ACHOS5_001211"
     CDS             26191..26529
                     /gene="cutA"
                     /locus_tag="ACHOS5_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9776061.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26505..28202
                     /gene="dsbD"
                     /locus_tag="ACHOS5_001212"
     CDS             26505..28202
                     /gene="dsbD"
                     /locus_tag="ACHOS5_001212"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9776062.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQMPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGSGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTVQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28239..28814
                     /gene="yjdC"
                     /locus_tag="ACHOS5_001213"
     CDS             28239..28814
                     /gene="yjdC"
                     /locus_tag="ACHOS5_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9776063.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPDHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            28921..28996
                     /locus_tag="ACHOS5_001214"
     tRNA            28921..28996
                     /locus_tag="ACHOS5_001214"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28954..28956,aa:Phe,seq:gaa)
     gene            29173..30357
                     /locus_tag="ACHOS5_001215"
     CDS             29173..30357
                     /locus_tag="ACHOS5_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9776064.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            30686..31267
                     /locus_tag="ACHOS5_001216"
                     /pseudo
     CDS             30686..31267
                     /locus_tag="ACHOS5_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            31354..31632
                     /locus_tag="ACHOS5_001217"
                     /pseudo
     CDS             31354..31632
                     /locus_tag="ACHOS5_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(31900..32904)
                     /locus_tag="ACHOS5_001218"
     CDS             complement(31900..32904)
                     /locus_tag="ACHOS5_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9776065.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKVLLRKKFTRTKLIEFLAG
                     CASSTVVMEACAGAHFMARRIADLGHEAKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEAQQAMRALHRVRESLVSDKVKTTNQMHAFLLEFGISMPTGVAVVKR
                     LSTVLAENELPPYLAQLLMRLHAHYIYLVGQITELEIALEQELSRDEVGQRLQTIPGI
                     GPITASVLSSQLGDGKQYSCSRDFAASTGLVPRQYSTGGKNTLLGISKRGDKNLRRLL
                     VQCARSFIQRIENQSGRLADWVRALLARKHSSVVCCALANKLARIAWAITTQQTVFER
                     "
     gene            32953..33908
                     /locus_tag="ACHOS5_001219"
                     /pseudo
     CDS             32953..33908
                     /locus_tag="ACHOS5_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            33948..34427
                     /locus_tag="ACHOS5_001220"
                     /pseudo
     CDS             33948..34427
                     /locus_tag="ACHOS5_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            34570..34799
                     /locus_tag="ACHOS5_001221"
                     /pseudo
     CDS             34570..34799
                     /locus_tag="ACHOS5_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34786..36288)
                     /locus_tag="ACHOS5_001222"
     CDS             complement(34786..36288)
                     /locus_tag="ACHOS5_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9776066.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWVSN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(36450..37211)
                     /locus_tag="ACHOS5_001223"
     CDS             complement(36450..37211)
                     /locus_tag="ACHOS5_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP9776067.1"
                     /translation="MKKIIAVSALAVVGMFSGLALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSNFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
     gene            38001..39044
                     /gene="subA"
                     /locus_tag="ACHOS5_001224"
     CDS             38001..39044
                     /gene="subA"
                     /locus_tag="ACHOS5_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP9776068.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHE
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMSEEL"
     gene            39061..39483
                     /gene="subB"
                     /locus_tag="ACHOS5_001225"
     CDS             39061..39483
                     /gene="subB"
                     /locus_tag="ACHOS5_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFP9776069.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPNRKKN"
     gene            complement(39871..40194)
                     /locus_tag="ACHOS5_001226"
     CDS             complement(39871..40194)
                     /locus_tag="ACHOS5_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFP9776070.1"
                     /translation="MAYHSPDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILFRLSKHHHLYEKK
                     KTKEG"
     gene            complement(40228..40419)
                     /locus_tag="ACHOS5_001227"
     CDS             complement(40228..40419)
                     /locus_tag="ACHOS5_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776071.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            complement(40429..40749)
                     /locus_tag="ACHOS5_001228"
     CDS             complement(40429..40749)
                     /locus_tag="ACHOS5_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776072.1"
                     /translation="MCFLANGMPDTWFYTDDASVTKEWGREYCDNVEVFPLCYMHDGP
                     VFRGRYIDELSAARSRMLHMHHILPERTRYRENIHDAECIREIHEAPLQAPGILQKYK
                     RTKS"
     gene            complement(41282..>45334)
                     /locus_tag="ACHOS5_001229"
     CDS             complement(41282..>45334)
                     /locus_tag="ACHOS5_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP9776073.1"
                     /translation="GGLIAVSELSKKVSGKTGRRLMTVSLVLSMILSALPGKASTVSA
                     EIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMIDFSSVSRRGSLTLLSQ
                     GYGVSAKHGGLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHRFSKLITEAAPADINI
                     SGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTVGTPWYSGNNLISSSP
                     GDTYNKSQGALASYGQMGDSGSPLFAYDSLSEKWSLAGVTLHNNGVNGQKNNWLLLPE
                     DYIKNIITADFDPIISFNKNSKEHMSWTYDAAKGVGRIQQDDQQFVMHGNLNGNLNAG
                     KNLYFTGANGIIDLKDNVNQGAGYLQFADDYTVTTSNASSWFGGGIIVNSGTTVKWGI
                     NGVSGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNNDSTAFSSINISGGN
                     SRVKLSGDNQIIPDNVSWGFRGGYLDINGKNTEFSRLQAVDYGAAIINSSTEKSLLTL
                     NLSPLKKDEIAVSVKALDMNAIFHGGYGTTGDLYKTTFYGPTQYYLLKKPKFGSVLMG
                     SLKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNMDIVIPELTGNDIL
                     TLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFS
                     LSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHIT
                     LEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGL
                     FTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGD
                     IISDKQATISFGTESGKEGILSEKASRGVAVGLLSGFNTAYRGAIHAPSASATVNNTW
                     WQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLV
                     VNKKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVIT
                     AIDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGE
                     AGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAF
                     KGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAE
                     AGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDYNPLIGRTGVDVGK
                     SFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRD
                     NVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT        11506 a        11442 c        11065 g        11321 t
ORIGIN      
        1 actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
       61 gtactactta attttgcttt cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt
      121 atacgagaaa tctttccctc tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca
      181 agtgctgaaa ttatcagcaa gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg
      241 attccggcgc ttacgtgacc aggaaaaatt ttacagcttg ataaaatgct cgcggtagta
      301 agccaactcc gccaccgatt cacggatatc atccatcgcc tgatgcgtcc cctgcttggt
      361 aaagccgtca agaatttccg gcttccagcg gcgcgccagc tctttcaggg tgctaacatc
      421 gagatagcgg tagtggaagt aggcttccag ctccggcatg tatttaaaca ggaagcgacg
      481 gtcctgaccg atgctgttac cacaaatcgg cgattttccc gcaggcaccc actgttttaa
      541 gaattcgagc gttgccagtt cagcttcacg atcgcccatc gtgctcgctt tcacacgctc
      601 taccagcccg ctggcggtat gggtgcgcac gttccagtca tccatcagcg ccagctgttc
      661 atcggactga tgtactgcaa tggtcggccc ttctgccaga atattcaggt tggcatcggt
      721 caccagcgtg gcaatctcaa taatgcgatc gcgctcggga tccagaccgg tcatctcaag
      781 atcgatccaa atcaggttgt tttcattggc actcatgcta ttttccaccc attcacagtg
      841 actatattga tccacaattg cgtgtatcat agatgttttg cccatcaggg gcgaccagga
      901 gtctgtacga ttgagtaaaa ataaactctc caaaggccag cagcgccgcg tgaacgccaa
      961 tcaccagcgt cgtcttaaaa cgtctaagga gaagcccgac tacgacgaca atctgtttgg
     1021 cgagcctgat gaaggtatcg tcatcagccg ctttggtatg cacgctgatg tggaatccgc
     1081 cgatggcgac gttcaccgct gcaatattcg ccgtaccatc cgttcgttgg tgaccggcga
     1141 ccgcgtagtc tggcgtccgg gtaaaccagc ggcggaaggc gtaaatgtca aagggatcgt
     1201 ggaagcggtg catgagcgca cctcagtgtt gacgcgcccg gatttttacg acggtgtgaa
     1261 acctattgct gccaacatcg accagattgt cattgtctcc gccattttgc cggagctgtc
     1321 gctcaatatt atcgaccgtt acctggtggc ctgcgaaacc ttgcagattg agccgattat
     1381 tgtgctcaac aagatagacc tgctggatga cgaaggcatg gagttcgtca acgagcagat
     1441 ggatatctac cgcaatatcg gttatcgcgt attgatggtt tccagtcata ctcaggatgg
     1501 gctgaaaccg ctggaagagg cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg
     1561 cgttggcaaa tccagcctgc tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa
     1621 cgatgtctct gacaactcgg ggcttggtca gcacaccacg accgccgcac gactgtatca
     1681 cttcccgcac ggcggtgatg tgattgattc cccaggtgtg cgtgagtttg gtctctggca
     1741 cctggagccg gaacaaatca ctcagggctt tgtcgaattc catgactatt taggtctgtg
     1801 taaatatcgc gattgcaaac acgataccga tccgggctgc gctatccggg aagcggttga
     1861 ggaaggaaaa atcgcggaaa cccgtttcga aaactatcac cgtattctgg aaagcatggc
     1921 gcaggtaaaa acgcgtaaaa acttttctga tacggatgac tgacaattaa gctaaccgtc
     1981 gttagaatcg tccccttttt ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa
     2041 caatggcctg gaggctacct tgttaaattc atttaaactt tcgctacagt acattctgcc
     2101 gaaactatgg cttactcgcc tggcgggttg gggcgcaagc aagcgggcag gatggctgac
     2161 aaaactggtt atcgatctgt tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa
     2221 gccggacacc gccagctacc gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga
     2281 agtacgccca atcgataccg atccgaatgt actggttatg cctgccgatg gcgttatcag
     2341 ccagttgggt aaaatcgaag aagataaaat cctgcaagcc aaaggccaca actacagcct
     2401 cgaagccctg ctggcaggca actatctgat ggcggatctc ttccgtaacg gtacgtttgt
     2461 gaccacttac ctctccccac gtgactacca ccgcgtacac atgccgtgta acggtattct
     2521 gcgtgaaatg atctacgtgc cgggcgatct cttctccgtt aaccatctca cggctcagaa
     2581 cgtgccgaat ctgtttgccc gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg
     2641 cccgatggcg cagattctgg tcggtgcgac gattgtaggc agcattgaga cggtctgggc
     2701 gggcaccatt acgccgccgc gcgaaggcat catcaagcgc tggacctggc ctgccgggga
     2761 aaacgacggt tctgtggcat tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc
     2821 taccgttatc aacctgtttg caccaggtaa agtgaacctg gttgagcaac tggaaagcct
     2881 gtctgtcacg aaaattggcc agccgctggc agtatctacc gaaacctttg ttacgccaga
     2941 cgctgaacca gccccgcttc ctgctgaaga gatcgaagca gaacacgacg ccagcccatt
     3001 ggttgacgac aaaaaagacc aggtctaatc catcaaagga aacgctgacg tgcgcctgat
     3061 tatcactttt ctgatggcct ggtgcctcag ttggggggcg tacgccgcga cggcccccga
     3121 tagcaaacaa atcacccagg aactggagca ggcaaaagcg gcgaaacccg cgcagccgga
     3181 agtcgtagag gcgctccagt ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga
     3241 gcgcatcaag caatatcaag aagtcattga taattatccg aaactttccg ccactctgcg
     3301 cgcgcagtta aacaacatgc gtgacgagcc acgcagcgtg tcgccgggaa tgtctaacga
     3361 tgcgctgaat caggaaattc tccagatcag cagtcagttg ctggataaaa gccgtcaggc
     3421 ccagcaagag caggagcgcg cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca
     3481 gcaaaccgat gcccgccgtc agttgaatga aatcgagcgc cgcctgggga cgcttaccgg
     3541 caataccccg ctcaatcagg cacaaaattt cgcgttgcag tctgactccg cacgtctgaa
     3601 agcgctcgtt gatgaactgg agctggcgca gctatctgcc aataaccgcc aggaattagc
     3661 gcgcttgcgc tctgagctgg cggaaaaaga gagccagcag ctggatgcat atttgcaagc
     3721 actacgcaac cagttgaata gtcaacgcca actggaagcg gaacgggcgc tggaaagtac
     3781 cgagcaactg gcggaaagta gcgccgattt gccaaaagat atcgtcgccc agtttaaaat
     3841 taaccgcgaa ctatcggcgg cgctgaatca acaggcgcag cggatggatc tcgttgcctc
     3901 gcaacagcgt caggctgcca gccagacgtt acaggtccgg caggcgttga atacgctgcg
     3961 tgaacagtcg caatggctgg gatcatctaa cctgctcggc gaagcgttgc gggcgcaggt
     4021 ggcacggcta ccggaaatgc caaaaccaca acagcttgat accgaaatgg cgcagttacg
     4081 tgtgcaacgg ttacgttatg aggatctgct taataaacag ccgctgctac ggcaaatcca
     4141 tcaggccgac ggtcagccac tgaccgctga gcaaaaccgt attctggaag cacagctacg
     4201 cactcagcgt gagttgctga actcattgtt gcagggtggc gacacgctac tgctggagct
     4261 gaccaagctg aaagtctcca acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc
     4321 gacgcaccgc tatctgttct ggacctctga cgtgcgcccg atgaccatcg cctggccgct
     4381 ggaaatcgcc caggatctgc gtcgtctcat ttcgctggac accttcagtc agttgggcaa
     4441 agccagtgtg atgatgctga ccagtaagga gacgattttg ccgctgtttg gcgcgttgat
     4501 tctggtcggt tgcagtattt actcgcgccg ctatttcacc cgttttcttg aacgttcggc
     4561 ggcgaaagtc ggcaaagtga ctcaggatca cttctggctg acgttacgca ctcttttctg
     4621 gtcgattctc gtcgcgtcac cgctgcctgt gctgtggatg acactgggtt acggcttgcg
     4681 cgaggcgtgg ccttatccgc tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc
     4741 gctgctgtgg gtagtgatga tttgcgccac ctttgctcgc ccgaacggct tgtttatcgc
     4801 tcattttggc tggccgcgcg aacgagtttc ccgtgggatg cgttactacc tgatgagcat
     4861 cgggcttatt gtgccgctga ttatggcgct gatgatgttc gataacctcg acgaccgtga
     4921 attctccggt tcgctgggac ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt
     4981 caccctcagc ctgaaaaagg ccgggatccc gctgtatctc aacaaagagg gcagcggcga
     5041 caacattacc aaccatatgc tgtggaacat gatgattggc gcaccgctgg ttgccattct
     5101 ggcgtcggcg gtgggttatc tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc
     5161 ggttgccatc tggtttttgc tactggtggt ttatcacgtt atccgccgct ggatgctgat
     5221 ccagcgccgc aggctggcgt ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca
     5281 gcgcgcgcgt ggcgaagagg aagcgcatca tcacagtagc ccggaaggag caattgaagt
     5341 cgatgaaagc gaagtcgatc tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc
     5401 aattttgatg ttgatcgccc tgctttcggt cattgtgctg tggtcagaaa tccattccgc
     5461 tttcggcttc ctcgaaaata tttcgctgtg ggatgtcact tccacggtac agggcgtaga
     5521 aagcctggag ccgattaccc tcggtgcggt actgattgcc attctggtgt ttatcatcac
     5581 cacgcagctg gtgcgcaacc tgcccgcgct gctggaactg gcgattctgc agcacctgga
     5641 tttaacgccg ggtacgggct acgccatcac caccatcacc aaatatctgc tgatgctgat
     5701 tggcgggctg gtcggcttct cgatgattgg tattgagtgg tcgaaattgc agtggctggt
     5761 cgccgcgctc ggtgttggtc tcggttttgg tttgcaggaa attttcgcca actttatctc
     5821 tggcctgatc atcctgttcg aaaaaccgat tcgcattggc gatacggtaa cgatccgcga
     5881 tcttaccggt agtgtgacga aaattaacac ccgcgccacc accatcagcg actgggaccg
     5941 caaagaaatt atcgtgccga acaaggcgtt tattaccgag cagtttatca actggtcgct
     6001 ctctgactcg gtcacgcgtg tggtgttgac aattccggcc cctgccgatg ccaacagtga
     6061 agaagtgacg gaaatcctgc ttaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc
     6121 ggcgccggaa gtcttcctgg tggatctgca acaggggatt caaattttcg agctgcgtat
     6181 ttacgccgct gagatgggtc accgtatgcc gctacgccat gagatccacc aactaattct
     6241 ggcgggcttc cacgcccacg gtatcgatat gccattcccg cccttccaga tgcgtctgga
     6301 aagcctgaat ggtaaacaaa cggggagaac attaacttct gcgggcaaag gtcgtcaggc
     6361 gggaagtttg taatgcatga agctgccaaa tgatgtcaac gtggctcatt tagcagctta
     6421 acatcatctt gccccggaga gtatcatcca tacgcattac ttcctgacaa ctcatcgccg
     6481 gagagacgat gagttgcagg atttcatatg atcagtcgag agctaactca ggtttgttcg
     6541 ccatgcgact gcaatagttc tggtaaatcg ccatcgctaa cagcgagaag aagacagggc
     6601 cgccaatcat ccacaatgtg ctgtcccagt ctccagcttc aaccacaggt tgaatgatgg
     6661 tgaagacgtt cgcaaatgtc accaccagta cgacaaccac tgttgcaatc attgccgaca
     6721 tatgcgtttt gaaaatcaca aacggtctgt cgagatcctg acgtgcttta aaaaatggga
     6781 aagccagcgc gaggaacagg taaggaagcg tcatagacac gttcgccatc agcgtcagct
     6841 tgttaaagaa cgccgatgcg gtaccgccac caaacgaaac cagcagaatg aagatagtaa
     6901 ccaacccgca ctgcatccac atggcaatag acggcatccc catcgtattc aggcgcgtca
     6961 tcggttccgg ccacaatgct ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc
     7021 aaagtgtaaa gaacgcaccg gtataagcga ggaacatcga cagtccggta atacgcgcaa
     7081 accatacgcc cagcgacaat gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca
     7141 cccctaggct cttcatcagc acataggtaa tattgccgag gttaacggaa ccattactta
     7201 atacctgctg ccagtttgtg ctgacgcccc ataaaaatat tgccagcgaa taaccgattg
     7261 aaataacaat agcggcaaaa acaatacctt tggcaaagtt cttttctgga ttttccgttt
     7321 tatcgaccag accaccaacc gcttcaattc cgccataggc aaaaatggca aataccacaa
     7381 acgatagcat tgccagaccg gactgataac ctgggttcgg tgatgcaagg aaattaatat
     7441 cctgcgcgaa atgcccacca tttaataaca aaatagtaat gcttactaac agcaatacta
     7501 aattcagaca cattactgca ataccgccca ccgcagtaat gcgggcaatt ttattaatcc
     7561 cttttgaagc aacgacggtg accagaatca tccatgccac tgccagcaga ccaaccacct
     7621 gcgtaggctc cagtccggca atatgccagt gctgggtcat gtcgctaccg tagaggaatg
     7681 ttgagaacgg tacccaaact ttcgcggagg tactcaccat ccagatgata taagaggaaa
     7741 accacataaa cgtaccaatg aaggcaaaac gtggtccgac actattattc atccaggaat
     7801 agataccgcc ttcttctttg cgataagcag ctcccatttc agccatcatt aaggcgaatg
     7861 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
     7921 aataagccga tgggctattg gcaaatccaa atacagaagt aaagatcatc aatatgagtc
     7981 ctatcagact catctttttt atcgagtgag gcatgaaacc atccttcatt tcagcgcgtg
     8041 tataaaacgc gcccactgaa ctgcactgcc caacattgag cgggcagcgt aaaaaccaat
     8101 acagaattga tacctggatg atatcaaata tccagtctta aattgtggcc tttagcggaa
     8161 aaatgaaaac atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
     8221 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
     8281 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
     8341 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
     8401 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgtcaattc
     8461 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
     8521 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
     8581 ggtcggtttt tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagaag
     8641 cgtgtcgcgg tcttcttcag tatcggcaac gttgctcaaa tccagtttcg ctgccacttc
     8701 ccgcagttgc gttttgtcgg cagatagtgg gtcaatttcc agataacgca agaaagcttg
     8761 ttgataagaa aggctttctg ctgccggaca gtccagcacc tgttgcaaga gatcgtccac
     8821 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc
     8881 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     8941 cccacaaccg gcaaccagca ggcgcttcat atggtattcc gggctggtca ttaaccagag
     9001 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     9061 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     9121 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     9181 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     9241 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaataaaa caacggtggg
     9301 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     9361 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ctatccatca
     9421 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     9481 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     9541 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     9601 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     9661 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     9721 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     9781 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc
     9841 ccatggagcc gtcgtccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     9901 gagcgtacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     9961 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
    10021 gttgatgatg gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg
    10081 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
    10141 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
    10201 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gcctaccagg ttccggtatc
    10261 ctgatgaccg aaggctgccg cggtgaaggt ggtattctgg tcaacaaaaa tggctaccgt
    10321 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
    10381 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
    10441 aacaccatct ccacgccacg tggcgatgtg gtttacctcg acctgcgtca cctcggcgag
    10501 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
    10561 gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc
    10621 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
    10681 tctgttggtc tgcacggtgc gaaccgtctg ggctccaact cgctggcgga actggtggtc
    10741 ttcggtcgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
    10801 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac
    10861 caggatggcg gcgaaaactg ggctaagatc cgcgacgaaa tgggcatggc aatggaagaa
    10921 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
    10981 ctgcaggaac gcttcaagcg cgtgcgcatc accgacactt ccagcgtgtt caacaccgac
    11041 ctgctctaca ccattgagct gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc
    11101 gcaatggcac gtaaagagtc ccgcggcgcg caccagcgtc tggacgaagg ttgcaccgag
    11161 cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact
    11221 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg tgtttacggt
    11281 ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc
    11341 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
    11401 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
    11461 ctacatcaaa gacaacctgg caccggacct gagctaccgc tggtcctgcc gtatggcgat
    11521 ttgtggctcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
    11581 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
    11641 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
    11701 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcgaa
    11761 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
    11821 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
    11881 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt
    11941 atggagctgt actttcgtgg gctactgctc cgaagtctgc ccgaaacacg tcgatccggc
    12001 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
    12061 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
    12121 ccacctggtg gaaaaaattg ccgttttatc gcttctacat gctgcgcgaa ggcacggcgg
    12181 ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc
    12241 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
    12301 tgatcactct ggcggcagcc ctgctgcaca ccaaaacctg gtttgagctg gcaccaaaag
    12361 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
    12421 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
    12481 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
    12541 ggggccggtg gtatgtggag cgccatcatt gcgccggtga tgatcctgct ggttggtatt
    12601 ctgctgccgc tagggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
    12661 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
    12721 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtacc tgcgggtaaa
    12781 tgggttttct acggtctggc tgctatcctg acagttgtca cactgattgg tgtcgttaca
    12841 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
    12901 cagaccctat gttcaaaacg acgctctgca ccttattaat taccgcctct tgctccacat
    12961 ttgccgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
    13021 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
    13081 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
    13141 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
    13201 aaatcaagtt aagcgatccc acaacaaaat actggcctga acttaccgct aaacagtgga
    13261 atgggatcac actattacat ctcgcaacct acaccgctgg cggcctgcca ttgcaggtgc
    13321 cggatgaggt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgtat
    13381 gggctccagg aacacagcgt ctgtatgcta actccagtat cggtttgttc ggcgcactgg
    13441 ctgtgaagcc gtctggtttg agttttgagc aggcgatgaa aactcgtgtc ttccagccac
    13501 tcaaactcaa tcatacgtgg attaatgtac cgtccgcaga agaaaagaac tacgcctggg
    13561 gatatcgcga aggtaaggca gtacatgttt cgccaggggc gttagatgct gaagcttatg
    13621 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta aaaccccttg
    13681 atatcaatga gaaaacactt caacaaggga tacaactggc acaatctcgc tactggcaaa
    13741 ccggcgatat gtatcagggt ctgggctggg aaatgctgga ctggccggta aatcctgaca
    13801 tcatcattaa cggcagtgac aataaaattg cactggcagc acgccccgta aaaccgatta
    13861 cgcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
    13921 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
    13981 aaaactaccc caatccagcg agagtcgccg ccgcctggca gattctcaac gctctacagt
    14041 aaaatttcat cgggtccgaa ttttcggacc ttttctccgc ttttccttgc tgtcatctac
    14101 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
    14161 acagctgcat ttctggtcgt tgcctgcagt tcccctacgc caccgcgtgg cgtgaccgta
    14221 gtgaataatt tcgacgctaa acgttatctc ggtacctggt atgagattgc ccgttttgat
    14281 caccgctttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
    14341 ggcctgaatg tcattaataa aggctataac cctgacagag aaatgtggca gcagagtgaa
    14401 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
    14461 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
    14521 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
    14581 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
    14641 tgggtacagc agcccggtag ttaatgagtg ctgagtttca aaccaataat ccccaatacg
    14701 attaacgcca gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg
    14761 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctactggt
    14821 aacgatttca tcgcccaggc aagtagcgcc agactgacaa tcatcgccgt cacagtaata
    14881 acacttggcg tcaaacgact aaagccgtga gtatatttca ggccaacggc ccataccact
    14941 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaaattgggg
    15001 ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
    15061 attttgccag gtggcagggg aatttcaact attacctatg taactatcgc agaaaaaaca
    15121 aacggcacga cacagctgtc gtaccgttat tgcttattgc ttattgctgc gctttcgttg
    15181 ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt
    15241 tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct
    15301 tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa
    15361 tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaggaga agaaaaatta
    15421 gctggcagcg cgggagatgg agttgccgag atgtttgata tcttcgccga aaccgcgagc
    15481 ggtgttacag cccgtgagca gcgcgctggc aagcaaaaca agaacgataa ggcgtttcat
    15541 catctctgac ctgaaaaagt gatggtgcag cctacaggcc gcaccacaac cgcattactt
    15601 cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc cgatctgtac
    15661 gaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc
    15721 tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg
    15781 cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg cctgatccag
    15841 caaccattta gcgttgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc
    15901 gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac
    15961 aacatcagcg ccttcagcag aatcagtaga tttgaaggtt ttttctacgc gagtaccggt
    16021 cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc
    16081 actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc
    16141 gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca
    16201 aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg
    16261 ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa
    16321 aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta
    16381 tcgatcgcat ggagaaaggc aatccggacg atcctctttt gcatcaggta cttacctcgc
    16441 aagatgagtt tgtcgtcgcg cccggattct ccaccgaccc gctggaagaa cagcatagcg
    16501 tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct
    16561 gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca
    16621 acaagcgtaa ctggcagact gcacttgagt atgttgctgc gcatccggaa ctggacgaga
    16681 tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca
    16741 cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg
    16801 tgatcccggc acgcatcaca gatgcgctgg ttgaacgctt ttctcattct acgctgcaaa
    16861 tcttgctggt gaatcacatc aaccatgcca atgaggtaga tgaaacattc cgtcaggcga
    16921 tggctaagtt gcgccgggtg ggcgttactt tgctgaacca gagcgttctg ttacgtggtg
    16981 tgaacgataa cgcgcaaacg ctggcaaacc tgagtaatgc gttgttcgat gcgggcgtga
    17041 tgccgtatta cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt atggtgagtg
    17101 atgacgaagc acggcagatt atgcgtgagt tgctgacatt ggtgtcggga tatctggtgc
    17161 ctaaactggc gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat ctccagctac
    17221 gccagcagta actgattaaa cagggaagtt taatctttgc ccgttgcata acctttaatt
    17281 attcatatac attgctgttg ttactcttta tataaccaac accaataatt aacctttctt
    17341 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
    17401 cccatttatc tggcttataa actaaaatta attatttaat tgagaaatgg ttagggagaa
    17461 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
    17521 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
    17581 ctgccatgga attgcgcgac cttctgattg cgctggaaag tcgtctgcat caaaagcata
    17641 aactggatgc tgctgcacgc cttcagtatg aacaagctcg cgataaagtt attaagaaaa
    17701 tggcagaaaa tatcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
    17761 gggttaacac gctggaactt accgatgatc agggcgaaaa tcttaccttc gtattaacgc
    17821 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
    17881 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
    17941 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggaaaatggc aatctggaat
    18001 atgacaccta ttcccaaccg gaatggaaac ataacttgtt caaccattat ctggcggtgc
    18061 tttaccgctt taaagatgaa agtggcaatg aacaatttag cggcgcagtg gtgaaaacgc
    18121 gtgaagcaac gccgggtaaa gaagtggagg cgatcactcg ccgaatgctt gatttcagcc
    18181 cccgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcaa
    18241 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgttc
    18301 aatccaccag caaaactgca ccacaggcga aataatctca tctgttttac tacgaggctg
    18361 gaagtatcca gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg
    18421 gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat
    18481 cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga
    18541 accgccttct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc
    18601 aatcagctgg cactctgcac ctggttgctc gtccacaatg cgtacactct gaccggcagc
    18661 actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc
    18721 taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt
    18781 ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc
    18841 cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc
    18901 atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta
    18961 accatgcact cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa
    19021 cgggttactt tggttggatc caggataccc atatcgatca tgttgccgta ttcttcagtt
    19081 gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac aacagacggt
    19141 tcttcgccgc agttcaggac gatctgacgc agcggagctt ccattgcacg cagtgcaact
    19201 ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg
    19261 cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg
    19321 tgcagggcgt cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacca
    19381 actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag tttttcacgg
    19441 tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt
    19501 gcagcttctt cacccacgcc atcgatgatg gtggtggtgt ctttgttgat cacaacacgt
    19561 ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag
    19621 attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg acgatcgccg
    19681 aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt aacaaccaga
    19741 gttgccagtg cttcgccttc aacatcttca gcgatgatca gcagcggttt acctgctttg
    19801 gcaacagctt ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc
    19861 aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga
    19921 gacaagtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg
    19981 gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc
    20041 agtttaccta cggtttcgtc ggagttagca gagatggtac caacctgagc aatcgcttta
    20101 gagtcagagc acggtacgga cagcgctttc agttcttcaa ctgcagcggt aactgctttg
    20161 tcgataccac gtttcaggtc catcgggttc atgcccgcag caacagcttt cagaccctca
    20221 gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc agcgtcgttc
    20281 gctttagagg caacttcttt caccatctgc gcacccatat tttcgaactt gtcttccagt
    20341 tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta
    20401 tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg
    20461 tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc
    20521 attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gcggattacg cttcaacaat
    20581 tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt
    20641 cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac
    20701 ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt
    20761 agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg
    20821 cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa
    20881 gtccgtatct attatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga
    20941 aatggggatg aggcttcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca
    21001 aaatcgggtg aaaaccctga ttcacctcac atttcatcgc tatttcttca tcaccgtcat
    21061 aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa
    21121 tcggacatca acccatgagt ggactcaaac aagaactggg gctggcccag ggcatcggcc
    21181 tgctatcgac gtcattatta ggcactggcg tgtttgccgt tcctgcgtta gctgcgctag
    21241 tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg
    21301 cgattgtgtt tgcgattctg ggtcgccact atcccagcgc aggcggcgtc gcacacttcg
    21361 tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca
    21421 ttcccgtggg tttgcctgcc gcgctacaaa ttgctgccgg attcggcctg gcaatgtttg
    21481 gctggcatag cgggcaactg ttgttggcag aactcggcac gctggcgctg gtgtggtata
    21541 tcggtactcg aggtgccagt tccagtgcta atctacaaac agttattgcc gggcttatcg
    21601 tcgcactgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc
    21661 ctgcgccagg aaatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt
    21721 gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg
    21781 attttcctcg tgctttgatg attggcctgc tgctggcagg attagtctat tggggctgta
    21841 cggtagtcgt cttacacttc gacgcctatg gtgaacaaat ggcggcggca gcatcgcttc
    21901 ccaaaattgt agtgcagttg ttcggtgtag gagcgttatg gattgcctgc gtaattggct
    21961 atctggcctg ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt
    22021 cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc catattccga
    22081 ataatgccct caatgcggtg ctcggctgct gcgtggtgag cacgttggtg attcatgctt
    22141 tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt atgatttatc
    22201 tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcagtgg
    22261 ttggcgggct attatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctacgcgc
    22321 tgatcatgct ggcggggtta tggctgtttc tgccaaaacg aaaaacgccg gaaaatggca
    22381 taaccacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg atcaaggcgg
    22441 tcgcggtcat catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg
    22501 gcgctaaaac cgccgcctgg catacgggaa aaacgcagat gaggcatcaa cttcactgtc
    22561 agatgctttt gcaccggcgg taataaaaga agcaaaccga ggaaatcggt gaaaaagccc
    22621 ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat ctccgccgct
    22681 gggtcttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta
    22741 cgtaccagtg acataccgat aactgacgtg aatataacca gcacgagggt cagcaatacc
    22801 cccaatacat gagcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg
    22861 gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggtt aaacccggct
    22921 acctgaatgg gttgcgaatc gcgtttagct tatattgtgg tcattagcaa aatttcaaga
    22981 tgtttgcgca actatttttg gtagtaatcc caaagcggtg atccatttca caaattaata
    23041 attaaggggt aaaaaccgac acttaaagtg atccaggtta cggtagaaat cctcaagcag
    23101 catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa
    23161 accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca
    23221 tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg
    23281 cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa
    23341 tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg
    23401 caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg
    23461 atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg
    23521 gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac
    23581 tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat
    23641 gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga
    23701 ttaaactggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat
    23761 tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg
    23821 gtcaggaatt ccgcgccttc agcatcctgc tgaaagaaga agtgaaaaat atccaacgta
    23881 ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca
    23941 cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat
    24001 gcgtaccggc tgaagacctg atcgaagcga cctccgactg cggcgcttat gttatggttc
    24061 acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc
    24121 tctcttctgg tccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcaggct
    24181 cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct
    24241 tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt
    24301 tgaacgttat ggagccagtc attggccagg ccatgttcga atccgttcac attctgacca
    24361 acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt
    24421 gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc
    24481 accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag
    24541 tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga
    24601 atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt
    24661 acagggtagt acaaataaag aaggcacgtc agatggcgtg ccttttttct tgtgagcagt
    24721 aacttaaaaa taacaattta atatcaactt gttaaaaaac aaggaaggct aatatgctag
    24781 ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag
    24841 gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg
    24901 gtaacatccc attcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca
    24961 tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta
    25021 acccgaaata catcacgatc ctcgcaccga tcgtgaccta tttcctgact atctttgctg
    25081 gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag
    25141 gcgttaaacc ttgccgtccg ctgtctactg cagtggtatc cgcgcagatt gcgatcaccg
    25201 catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca
    25261 gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt
    25321 ccttcctggt cactatgctg ttcaactcca aactctctga cgacccgatt tatcgcaagc
    25381 gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg
    25441 caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca
    25501 tcaacagccc aagcatgggt ctggttgaaa aaccactgat gaacaccacc aacgcaatcc
    25561 tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca
    25621 acattctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg
    25681 ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgcag
    25741 gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc
    25801 tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg
    25861 tttctccgct gaccgctgtt gcttcttttg ctgcggtgtc tggtctgttc attctgccga
    25921 cctacccgac actggttgct gcggtacaga tggatgacac gggtactacc cgtatcggta
    25981 aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt
    26041 gcttcggctt cgtgctgggt agcttcatgc tgtaatgacc catcgcgggg cgttcacgcc
    26101 ccgctttctt tcccgccgac taacatcctt tccccgtccg ttgtatagtg acctctctct
    26161 tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccacg
    26221 tctgtcgtgg tgctatgtac ggcaccggat gaagcgacag cccaggattt agccgccaaa
    26281 gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat
    26341 tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta
    26401 tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa
    26461 cttctggttt tacctgttac acacggagac acagattacc tctcatggct caacgcatct
    26521 ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg
    26581 gacgttcaca atttgtcccc gcggatcaag cctttgcttt tgattttcag caaaaccaac
    26641 atgacctgaa tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc
    26701 gcattacgcc ggaacacgcg aaaattgccg acgtgcagat gccgcaaggc gtctggcatg
    26761 aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca
    26821 tcaaccaggc gagtgcggga gcaacgttaa ctgtcaccta ccagggctgt gctgatgccg
    26881 gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg
    26941 aagcgtcaca gcctgtgtct gttccgcagc aagagcagcc caccgcgcaa ttgccctttt
    27001 ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa
    27061 tgtacccact gatttctggc atcgtgctgg gcggtaaaca gcggctttcc actgccagag
    27121 cattgttgct gacctttatt tatgtgcagg ggatggcgct gacttacacg gcgctgggtc
    27181 tggtggttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca
    27241 ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctgc
    27301 aactcccctc ttcgctgcaa acacgtctca cgctgatgag caatcgccaa cagggcggct
    27361 cacctggcgg tgtgtttatt atgggggcga ttgccggact gatctgttca ccatgcacca
    27421 ctgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggca
    27481 gcggcacgct ttatctttat gcgctgggca tgggcctgcc gctgatgcta attaccgtct
    27541 ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg
    27601 gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt gatatatggg
    27661 gattacgctt gtggtcagcg cttggtgtcg cattctttgg ctgggccttt atcaccagcc
    27721 tacaggccaa acgcggctgg atgcgcgtgg tgcaaataat cctgctggca gcggcattgg
    27781 ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgtg caaactcaga
    27841 cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcaggcg ctcgttgaag
    27901 ccaaaggcaa accagtgatg ttagatctct atgccgactg gtgcgtcgcc tgtaaagagt
    27961 ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac
    28021 ttcaggctaa cgtcactgcc aacgacgcac aagatgtggc gctgttaaag catcttaatg
    28081 tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac
    28141 gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt
    28201 gaacgacact tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg
    28261 gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg
    28321 ctgaacgtgt ggattaccca ctggacgagc tacgccgctt ctggccagac aaagaggcaa
    28381 tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga
    28441 tgctggacga aacacaaacc gccgaacaaa agttgctggc acgttatcag gcgttatcgg
    28501 agtgcgttaa aaacaaccgc tatccgggct gtctgtttat cgctgcctgt acgttttatc
    28561 ccgatcctga ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt
    28621 tcacccacga actgttaacc acgctggaag ttgacgatcc ggcgatggta gcaaagcaga
    28681 tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg
    28741 tcgacaccgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg
    28801 gtgctttgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga
    28861 atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt
    28921 gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt
    28981 ccgagtccgg gcaccacttt cacttccaga ttcatctccc gaactccacg aaactcaagt
    29041 ataatcacat cgttacaagc aatctgaaca tcctgaactc caccgaactc tactgaactc
    29101 tttctcaatc aagctttcat tgggggcacc ggtgggggct cattatgttc agtcagtttc
    29161 aggagccccc atatggcact tacagccaga caggtcgaaa cagccagacc taaagaaaaa
    29221 gactataaac tctctgacga acgtggttta tatctgctgg taaaaaccac gggagcccgc
    29281 tactggcggc ttaaataccg gatagcagga aaagagaaaa aactggccct cggcgtctat
    29341 cccgacgtct cccttgctga ggccagaatc aaacgtgacg atgcccgaaa aatcatctcc
    29401 gaaggtggtg acccgggcga aaagaagcga aaggaaaaac tcactcagaa aatctctgcc
    29461 accaatacgt tccatgccct cgctacggaa tggcaccagc ataaatcttt gtcatggtct
    29521 gaaagttacg ccagaagcgt actggaagcg ctggataaag atattttccc gtatctgggc
    29581 aaacgaagcg ttacggatat cctcccgctg gaaatgctgg aaattctgcg ccgcatagaa
    29641 aaacgtggct cgctggaaaa actgcgtaag gtacgtcaat actgtaatca gatttttcgt
    29701 tatgccatcg ccaccggacg agccactgtc aatccggcat ctgaactgac cagtacgctg
    29761 gcggcgccaa aagctgcaca cttcccccac ctgagagcag atgagctccc tgtttttctc
    29821 aggaagctcg ctgaatatca tggcagtcct gttacccgca tggcgacaaa tctgctgctt
    29881 ctgacaggcc tcagaacgat tgagctacgg tccgctgaat ggtcagaaat tgattttgat
    29941 aatgccctgt ggacaatccc tgaaagccgc atgaaaatgc gacgtaaaca tgtcgtacca
    30001 ctgtcacgac aggtcactga cattctgcaa caactcaaaa ctttctccgg acaataccgg
    30061 ctggttttcc cgggacgttg tgatatcaac aagccaatga gcgaagccag catcaatatg
    30121 gtgctcaaac gtatcggtta cgatggcaga gcaaccggtc atggttttcg tcacaccatg
    30181 agtaccattc tgcacgaaca ggggtttaat tctgcctgga ttgaaatgca gttagctcat
    30241 gtggataaaa attccatcag gggtacctat aatcatgccc tgtatattga tggtcgccgt
    30301 gaaatgatgc agtggtatgc tgactacatt gattcgcttt ctgagcaggt aagttaagct
    30361 gagaacacgt tattctgatt ttcactcact tcacgattat tcctccggcc tgcaggttcg
    30421 gaggattctt atttttatgt tttattttca gataaagttt ttgacaaaac gtttattctg
    30481 catcagcaaa attatcacaa aacaacgcaa tcacagacaa attaacattt ttcagacggg
    30541 tatattgagt gcccacaatg taagacaggc aggcatccgg tgacatggta aaatattaat
    30601 ccagggcaaa aacaaggctt gatacacaac atcatgactt ttatgctctc agactatgcc
    30661 accacagatt ttacaggctc agaaaatgaa tgacagatta cgctttgaag tgaacgataa
    30721 ccagggctgt ttcgtgttcc cggacgcctg gtttggctcg ttgctggatg agtttgacga
    30781 actaattgat gcatacgaca gagatgaaat cagcgaaaca agttatatca acaaactcag
    30841 acgccttgcc cgacaggagc ctgactttat tgatgtacac gcttacctgg catgcgcatt
    30901 tctggagcag aatgccccgc gtaaggcact gaacgctgct cttaaaggac tggcagcagg
    30961 taatcgtctc atcccggaaa gtttcagtgg tgaaatcatc tggatgcatc ccgaaaaccg
    31021 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgtcatca
    31081 ggatgcagct ctgctcactg ataaaatact ggcatacaat cctgaagaca atcagggagc
    31141 acgctggcta ctgggaccgg aactgttacg tactggcgac catgaacagg cacgccatgt
    31201 actgaaagcg catgcagatg aattttcacc ttactggtat gaactcggcc ttctgcattt
    31261 tctgaaccgg ccccttgaaa cgccagacag tggatgtatc ttaaaataag agataggctt
    31321 ggatatatgt ctaacactag ttctaatttt gagatgactg gaattttgct ggggcaggag
    31381 cttcgtaaac gtaaaactcc tcaggaaaaa atcgccatta ttcagcagac aatggaaccc
    31441 ggtatgacgg tgtcccatgt ggcgcgtttg catggtattc agcccagcct gttgtttaag
    31501 tggaaaaaac aatatctgga aggaagtctc actgctgttg ctgccggaga ggatgtcgtt
    31561 cctgcctcag aactggctgc cgctataaaa caaataaaag agttacagcg cttgctgggt
    31621 aagaaatcta tggtccccac catttttgca acactgattt tgattgatgt tggcttgcct
    31681 aaatctatcc ggcgtcatta tgggcatgga tgcccgcgcc tcgatgagtt ccgcacctga
    31741 tgagcccaaa aaaccatccg gcttcagtga gccattttct tgctgaggct tttcatcagg
    31801 ccgatatgcc gttcatgtca tcaataatca gtcttcgcaa aaccagattg ggaactactt
    31861 taattcatga ttaacctgcc agccagactg gcgccatcgt tacctttcga acacagtctg
    31921 ctgtgttgtg attgcccacg ctatccgggc taatttgttg gccagtgcgc aacatactac
    31981 gctggagtgt tttcttgcca acaacgccct gacccaatcc gccaaccttc ctgactgatt
    32041 ctcgattctc tgaataaagg atcttgcgca ctgaacgagt aaccgtcgca gatttttatc
    32101 tccacgttta cttatcccta aaagtgtatt tttaccacct gtgctgtact gccggggtac
    32161 cagaccggtt gaagcagcaa agtcccgact gcagctatat tgtttaccgt ctcccagctg
    32221 cgatgacagt acgctggcgg ttatcggacc aataccgggt attgtctgaa gacgttgtcc
    32281 tacctcatca cgactcagtt cctgctccag tgcgatttca agctcggtga tctgtccgac
    32341 aagataaata taatgggcat gtaagcgcat cagtaactgc gccagatagg gaggaagttc
    32401 gttctctgca agtactgttg aaagtcgttt taccactgcg actcctgtcg gcatactgat
    32461 gccaaattcc agcaaaaaag catgcatctg attggtcgtt ttgactttat cactgaccaa
    32521 tgattccctg acccggtgaa gcgcccgcat ggcctgctga gcttctgttc gtggttgcac
    32581 aaatcgcatg gagggacgag atgccgcttc gcatatggct tctgcatcga caaaatcgtt
    32641 cttgttgctc ttaacaaaag ggcgaacgaa ctgcggagaa atcaattttg cttcatgacc
    32701 taaatcagca atccggcgag ccataaaatg agctccggcg caggcttcca tcacaacggt
    32761 agacgaggca cagcctgcca gaaactcaat aagtttggta cgggtaaact ttttacgcag
    32821 caataccttg cctgacttat cttggcagtg aatgtgaaag gaatgcttgc caagatctat
    32881 accaataagc gtaatgtttt gcatgatggt cttctccatg aaagaaaaac actctactag
    32941 ggtacagctc acagggtggt ggggaccatc tcattaacca tggaagttga gatcctcaaa
    33001 gaagcggtgg agtatggtca gtcgcgaaaa tggatagcgc atgcgccctt gttgccaacg
    33061 gacaaggaat aaccctggta agccgtattc tgggcgtgtc gcgtgcgcaa ctggcacaac
    33121 ggatgaaaac atctggtaat aagccgaata aacgtcgtcg cagatgtgat gaggctgcgg
    33181 atgcggaagt gttagcacgt atccttgata tcatcagcga tatgccgaca tatggctatc
    33241 gtcgtgtctg ggccattctc agacgacagt cccgtaacaa aggagtgccg ttcgtgaatg
    33301 caaagcgcgt ttatcggata atgagcgaaa ataacctgtt gttacatgac aaaccttcac
    33361 ggccgcagcg ggaacataaa ggccgaatat cggtgaagga aagcgaccag cgctggtgtt
    33421 ccgatggctt tgagttcggt tgtgatgatg gcgcaaaact gcgtgtcacg ttcgcactgg
    33481 actgttgcga tcgtgaagcc atagactggg ccgcaagcac gggaggttat gacaaagcaa
    33541 cggtgcagga tgtcatgtta ggagcaatag aaaaacgctt tggcgataaa ggagcggaac
    33601 ggccaatcca gtggctgact gataacggtt cggcttatac agctcatgaa acacggcagt
    33661 tcgccagaga gttgaatctg gagccctgca tgacggcagt cagtagccct cagagcaatg
    33721 gaatggcaga aaggttcgtg aaaacgatga aagaagacta catcgcgttc atgccgaaac
    33781 cgaatgtaag aacggcattg cataaccttg cagtggcgat cgagcattac aatgaaaccc
    33841 atccgcatag tgcgttaggt tatctctcac caagggaata tcgacgtcag cgggtaacgt
    33901 caacttaaga tacaacgact gtctgaatat cgtgggtcaa ggacagcctt ctgcattttc
    33961 ttaatgggga actggtaaaa gctgcaactg catttcgtcg cggctttgcg gcaaatactt
    34021 acatcgcaga aatactgtgc ggaaatctgc atccatttcc acttgctgta tggcataact
    34081 tttcaggcgg tccggatact gctgaagatt attatgccac ataccacccg ttatgggggc
    34141 aatatccgga agcattgctg tttgtaaact ggctctataa tcattcctct gtgctgcatg
    34201 aacgggcaga aatcattaaa tgcgccgaaa tgctgatgca ggaagatgat ttcgaaatat
    34261 gcgaaagtat tttaagacag caggagaagt tgcgtgaaag aattggtgag acgctttctg
    34321 agaaaattgt acagaaatgc agaaatatga atggtgaata tgtctggccc tggatattgc
    34381 cgttttcagc ggcaggcatg aaacatactg gcatacagta tcagtagata ttgcattagt
    34441 gtatcctgca cacaagtaat aatttatcca ccaataataa cactgttaat gtccccttcc
    34501 cctggttgtc agccaggggt tatcttctga atatttcttt tgaaaaggat aacacagtaa
    34561 attattttta tgaattatcc catggactca ttaacaccct ttcataatgc tttattgtca
    34621 aacacgttat ggctgacatc aaaaaaaccg gatttcctct gccagcgggt aatcacctcc
    34681 ccggtgtttt cggttggtct ggttactcca gtctggtaat tagcaagata atcgatataa
    34741 acagtggaaa actcatcgta cataatctgg tgatgaacat tacgcttatt ttcccctgac
    34801 cggaagaatc agaggctgcg gtttcagact gtctgccggt acattcctct ccccgttaaa
    34861 aaccataacg ggttcattat cttcgtctgt cagcggattg aatggcggta tattttcagt
    34921 acgaatgccg gtcagccact gaaaaatacc tgcgaaatga cgggcactga ttttcctgct
    34981 gacggactga tgagacgtga tgtcactggc ggtaataatc aggggaacgc tgtagcctcc
    35041 ctgcacatga ccatcatgat gaacaggatt ggcactatcg ctgaccgaca gcccatggtc
    35101 agaaaagtaa agcatggcga aatgacggga atgccggcga aggataccat caagctgacc
    35161 gagaaagtta tccagtttac tgatgctggc gaggtaacag gcaatctttc ggggatactg
    35221 ctccaggtaa tgattcggcc aggagttaag tcggtcacac ggattcggat gagagccaat
    35281 catgtgcagg aaaatcacct tcggagagga tttatccgcc agcgcacgtt ctgtttcctg
    35341 taacaacaac atgtcatccg ttttacggga agcaaagctg cctttcttga ggaaaacggt
    35401 atgctccgca tcagaagcaa taacagagat gcgtgtatca tgctccccca gttttccctg
    35461 attggatacc caccatgtgc tgtatcctgc ttttgctgcc agcgctacca cgttgttgcc
    35521 ggagtcaggg ttctgctcat agtcataaat cagtgtccgg ctcagggaag gtacggtact
    35581 ggctgctgcc gatgtatagc cgtcaataaa taaaccggga gccgtattca gccacggtgt
    35641 ggttggtacg ggataaccat ataccgacat ataatccctg cgcacactct caccagtgac
    35701 aatcacaatc gtgtcataca acggtgctcc cggcaggatt ttccagctgt cagccctgtg
    35761 ctgactcagt tgttcataac gctgcatttc acgcaatgtg tcagttgtcc ccacaacggt
    35821 tcctttaacc atccgcaacg gccagctgtt tacagagcat aatacgaaca gcagcagtgc
    35881 cagccagtta cggtgaccgc ggtggtgtgt ccgccagaaa ataaccatga acaccagaat
    35941 cgcggcactg accagaaaat gataaacagg aatcatcccg gtaaactccg cagcctcatc
    36001 cgccgtggtc tgcaacaacg cgacaataaa actgttgttg attttaccgt acgtcatacc
    36061 cgcaggcgca tacagtgcac aacagaacag aaataacaga gctgtaatgg atgtaagggt
    36121 atttctgtgt gcaaggagca gaagaaggaa cagcagcaac acattcccgg tggcattctt
    36181 ctcagtgtat ccgcatgcaa ttgtggttat gacagaaata acaaaaaaga ataaaaacaa
    36241 taaaatccgt ggtgtgttgt ccggacaaaa cagttttctg atattcatcg gagtatatcg
    36301 gcaacattat tatgaagaga ataaaataat aagaaaaaca gaaatatcct gtaaagcaga
    36361 taacagacaa ccctgcagta taattacggc agggagtttc cgtttaatta cagaagacag
    36421 gtaattatta tctttcagga gtagtttcgt cagaagttat aagtcacacc aagcatgatg
    36481 tcatgacttt taacatcaac ttctgattta tatttatcgc cccactcatc cttgtaactc
    36541 acactggcat cacctgcatc aagatagcga tagctgaggt ccagagcgat atccggggtt
    36601 acgtcatagc ggacacctgc cccaaaactc catgcaaaat tgttatcata tcccgggtaa
    36661 tgcaacgcag aaaaattgct cacctcacca gatttattcc aggaggtatc agtatatgtt
    36721 gccttatgat gtatccgtgc ataacccagt ccaacagaca cccatggagt gaatgcactg
    36781 ctggtgaatg cactgctgtt tctgaagtca taatacgtat tcaccatcag agtattcaca
    36841 ctgagcctgt tctgtgtgtc ttcccgacca ccaccgacca tcggagagta ccatgtatcc
    36901 agtgtataac gggagtccgc agctccgcgg ccatagaatt ccacttccgt gcgcacagga
    36961 acattgtaat gctgataaaa atcatagccc accgcaaggc cggcaccgaa taccgtatcg
    37021 ttcttatcgc cgcccttata cttataaaca tcgtccccaa agtcctgacg aaagcgctgg
    37081 tcagtctgag tcacaaccga agcaccagct ttaccggtca tataaaagcc tgttttgcct
    37141 ctatcagcca gggccaggcc tgaaaacatg cccaccacag caagcgctga aaccgcaata
    37201 atctttttca tttcagttat ctccgctatc actttccccg aacgataaac gtggttgctt
    37261 attctaaagc tatgcaaaag acagggtaaa aagagaaaaa tcgatcgaat cgatcgacaa
    37321 aatcgatcac gcatgatgaa gatttcttat cgaatacata aaaatagtga tggcaatcgt
    37381 ggcaggagct tacgtcaggc cctgaagtac tgacacaggc attgatggca ggtgatataa
    37441 ggagtgctcc agggaatgac tcccgactga cccaggcact gtcccgttgt caacccgtat
    37501 ggttactgct ctaactcatt tcctgtcgtc agacgttcaa ctctggacca ctggttatga
    37561 cgacaggtgt cgctcatttg tcagcactat aacggtcaaa gatcgtggca ttgacacatt
    37621 ctccagaggt aaatttctca agaggtaaca actctgccag gaggatatct gcagagagtt
    37681 acattcgttt gggttcatat ctgtgccgcg cgttttcctt ccatcagtga acaaacattc
    37741 agtcccatga cgaccgggca aatagcgtgt tcacagatat tgctgtccag gtacgaagta
    37801 ccataatgga tattggtaag ttattgggtg aaccccctcc atgaaaaaac caaatataaa
    37861 aatgaatgtt tcgggagaga cttcactcgc tttattgata gtgattgttt atcttttact
    37921 gaagacagga tatttcagat gtgatatccc tgtaacatat tgaccagcaa tactttttat
    37981 ttgtaccact ggaggatatt gtgctgaaaa atttacggct gcatattctg ttcctgcttt
    38041 ttatctcagt gtcagtcagg gcggaaaaac catggtattt tgatgcaatt ggtctgacag
    38101 aaacaaccat gtcgctcaca gacaaaaata ccccggttgt cgtatctgtt gttgattccg
    38161 gagtggcatt tgtaggaggc ctcagcgaca gcgaatttgc caaattcagt tttactcagg
    38221 atggttcgcc attcccggta aaagagcctg aagctttata tattcatggt actgccatgg
    38281 cttcactcat tgcctcacgt catgaggttt atggtgttta tcctcatgca ctgatatcca
    38341 gcagaagggt tattcctgac ggtgtacagg actcatggat tagagcaact gaaagcatta
    38401 tgtcaaatgt ttttcttgca ccaggagaag agaaaatcat taatatatcg ggaggccaga
    38461 aggggatatc ttccgcatcg gtctggtccg aactgctttc ccgtatgggc cggaataatg
    38521 agcgattaat tgttgcggca gtgggtaatg atggcgcaga tatacgaaaa ctgagtgctc
    38581 agcagagaat atggcctgcg gcttaccatc cggtcagcag tgtgaataaa aagcaggatc
    38641 ctgtgataag agtggcagct ctggcacagt accggaaagg agaaacacct gtattgcatg
    38701 gtggaggggt caccggaagt cggttcggaa acggctgggt tgatattgcc gcaccgggac
    38761 agaatattac attcctcaga ccagatggaa aaacggggat cggtagtgga acctcggaag
    38821 caacagctat agtttccggc gttctggcag cgatggtctc atgtaatccc cgtgcaacag
    38881 cgacagaact gaagcgtaca ctgctggagt ctgcagacaa atacccctca cttgcagata
    38941 aggtgacgga agggagagtt cttaatgcag aaaaagcaat tagcatgttc tgcaagaaaa
    39001 attatattcc tgtccgtcag ggaaggatga gtgaagaact gtaaaattcc ggaggtgaat
    39061 atgacgatta agaggttttt tgtatgtgca ggtgttatgg gatgtttatc ccttaatcct
    39121 gccatggctg agtggactgg cgatgcccgg gatggcatgt tttcaggcgt tgttattgat
    39181 cagtttcata cagggcaaat agataataac ccttattttt gtattgaagg gaaacaacct
    39241 ggtggttctt ccataagagc ctgctcgatg aagaattcat ctgtctgggg gccttcgttt
    39301 tccacattat ataatcaggc actatatttt tacacaacag gtcagttggt caggatttat
    39361 tatgaacccg gggtatggac gtatccgccc tttgtgaagg cattaacgtc caatgccctt
    39421 gttgggttat caacctgcgc cacatcgaca gaatgttttg gccccaacag gaaaaagaac
    39481 taataaatga tgctcctgcc cagatatata ctgggcggga gcatactttg gttgcgacag
    39541 aaatatgtca acaccacgaa ctttgacatt acgtcttata tcacactggc actgacaagc
    39601 cagtattgct aatgcaggag gatgcttttt caactcgcag gagtttcagg agctgcctga
    39661 tggcgctgaa atgatgctta ctcatgtggt ggctggtcac tgcccctgtc cgcgagctgg
    39721 cacgcaatgc actactgatg gcagtctggc gcgtaagcct gcataaaaca ttattgtact
    39781 atgtgtatca acgggttacc cggttagaac agccagtaca aaaatgcccc cgcagtgagc
    39841 atggcaagtt ttccccatac aatgggtttc tcacccctct tttgttttct ttttctcata
    39901 taaatgatgg tgtttactca gacggaagag tatttcgtct gtgccagtct tccggattat
    39961 cctgctcatt tgttcatgca tccggatacg tttaatacag aagtcgattt tagcaactgc
    40021 caggtttttg ttgagagtca gcgtggcagc atgatatgca tgctgccaca atattaatgc
    40081 ccgggcataa tcatgatgta tttccgcatc ttcagctgct gccgttatct gcgcccagga
    40141 catccggcgc agatacggat aatgcagtgt gacatccggg gaatggtatg ccattgaata
    40201 ctcctgtgga gaatcatcat ctccggttta ttctccgccg gggacgcaga cgccccggag
    40261 agcgaattct cccacatttt cttttttcag cgcgctgaag gcatcccgac aggctttttc
    40321 agtacggaac tcctgactgt gaacggccag tgtgggttgc cgggcatcgc cggaggagac
    40381 attaaagggg gatgagagcc agaaaatcag tatccacata gaacagcctt atgattttgt
    40441 gcgtttgtat ttttgcagaa tgccgggagc ctgcaggggc gcttcgtgaa tttcacggat
    40501 acattctgcg tcatggatat tttctctgta ccgggttcgt tccggcagta tgtggtgcat
    40561 atggagcatt ctgctccgtg cggcgctgag ctcatcaata tagcgccccc ggaaaacggg
    40621 accgtcatgc atatagcaca gagggaacac ttccacattg tcacaatatt cccgccccca
    40681 ctcttttgtg acggaggcgt catcggtgta aaaccatgtg tcgggcatgc cgttagccag
    40741 aaagcaaatg ttccatacat cgggtaccgt agacgtctgt catcattcat acgggttatc
    40801 tcattctatg ggctgaatgc tgctcagaat cagcctgctg agactgtcag ggctgccgca
    40861 gcgacgaccg gtcgttttgc tgtagcattc ccggcgtgtg tggcaccagg cggccggtgt
    40921 ttcatgaaga cgaacgcgcc gtgtttgttc tttgtggata aggagtccgg tgtgagtttt
    40981 tatgtttttc atgtgctttc cgtttcgttc tctgtgacag ctgtgtcaca catgaagggg
    41041 gaatgtaaca ttcagaatct tactgaaaat gtgccatttg tgggcaccgg tgatcaatcc
    41101 tgtattcagg ttctgtgtca cagttccgga taagtggaat atatggacgt aaaaagccga
    41161 cattgctgcc ggctttggtc atggcggtta agtaaacaaa tcgactgata ccccggaaat
    41221 gtatacccgg gcggcaggtc ctgagcataa agggccctca ggcccttttg aatacggagt
    41281 atcagaacga gtaacggaag ttagcgttga cagcattatc aacgttgtac ttaccaaagg
    41341 cggatttctc aaactccagt ccaaagcgga cgttatccct gatttctgca ttcaggccaa
    41401 cggacatcag catacggctg tccttttcac ctttgatacg tttttcacct gatgcgtccc
    41461 gcaatacagt ttcgccgtta gccagcaggt cgaactggta gcccagaccg gcacgggctg
    41521 tcactttcca gtccttacct gagaaggatt tacccacatc cacaccggta cgaccaatca
    41581 gcggattgta gtccctgtcc ttcatggaca gatgcatccc ctggtccttc catgcaaact
    41641 gtttaccgga tacagcaccg taaaccagtt ctgcctgtgg ctcaatccag gtatcctcag
    41701 tgacatgaca gcggtagcct gcttcagcac cggcatacca tgaatgcgta ctgtaatcac
    41761 gggttccgag tccggcaaag gttgccgtgt actcattatc atgatgaaca tacttaccaa
    41821 tcaggtcgat ataggcaccg gaatcaaaca tggcagaagc atagagtccg gcccccacag
    41881 acttcgtttt acctttgaaa gcatcagcgg aggcactgct gtcagtgtgt gtgacagtga
    41941 agccggtaaa caaatccagt ccgtccagct catgtttttt gtcgacaccg acctgaacat
    42001 gtgtgtggtt gtcactgaaa ccaccaccgg cagagccggt tccgctcatg atacgtgccc
    42061 atgcgccagc ttcaccattg atgtcacgca ggtcacccat acgtttattc aggttgttga
    42121 cctcactcaa aaatgatttg taccccacag atgcaaggcc tgaggcactt tgggttgcgg
    42181 caggattttc tgccatcctg tacccggtca ggttccatgt tgttttatct ccggcgtcta
    42241 tagctgtaat aacaggtgtg acattgctga aacctattgc ctgttcactt cctttaaaaa
    42301 catccttact gctgttcccc ggtgcactca ccagttcaat atccagtttt tctccggtgg
    42361 gtttgttcag aaaatcaacc agcagaatat tgtccttacc tgacagcttt ttatttacta
    42421 ccagcttatc cgcatttttc aggtccgtac gcatggcata cgcagtgcca ttcgtcgtca
    42481 gctcatcaac cgtcagtgta tggaatgctt tattcgctgc acttcctgta aaatatgtca
    42541 tgcttccggt attttttaac gagcgaagtg aggagtctcc tgtcagttgc caccaggtat
    42601 tgttcacagt ggcagatgca gacggggcat gaattgcacc gcggtatgcc gtattaaaac
    42661 cactcagtaa tccgaccgca actccccggg atgccttctc agacaatatg ccctctttac
    42721 ctgattcagt tccgaagctg attgtggcct gtttatcaga aataatatca ccaacaacag
    42781 atgcctggtc tctggctttc agtactgatg aatcagacat cagtttaaat ccgccatcag
    42841 ccatattgat ggtcggtaaa aaagcagcat catctgaatt cactggtgta ccggtaagca
    42901 gaagggaagc gtcaccaata tcaatgcttc cacttgtaaa cagccctccc tgagcggtca
    42961 gcgcacctcc tttatgaata ttcagggagc tgtttataaa tgacgatgca tctgagaaca
    43021 ggactgaaga tgaagaaaca ttgatatgac tgtcttcaga cgtaaccccg ccacgaaaat
    43081 tatcgcggat atccagtgct gatttttgct ccagagtgat atgccctgta taggaggatg
    43141 ttcctgcggc agcagatttc ccttcaacac tgatgacatc attacctgtt ccgtcatttt
    43201 tatcagtaaa gactgtatct ccgccgataa taacagtgct ctggttaacg gccctgatat
    43261 tcccgttcat aaatgcatta cgggacagac tgaagtccgc attattaaga ttcagattgt
    43321 tgagtgagaa ctccctgttc tcccagtcac tctgcgatgc agaaacagtt tgccctgcat
    43381 gaataaccgg gtgtccctgg aagataagag caccgtcctg ttttgacata tctcctgata
    43441 tactgacaga gccatcaagc gttaaaatat cattgcctgt aagctccgga atgacaatat
    43501 ccatattccc caggagctta ccgtgataaa gatatgaagc ctgtgcactt gatgtcagtt
    43561 tattattctt tgccatatca acggcctgat tcaggtctgt tcctgcaaat tgccattcgc
    43621 tggtgttttt cagtgacccc atcagcacac tgccaaattt gggctttttc agcagataat
    43681 actgtgtcgg accgtaaaaa gttgttttat acaaatcgcc ggttgtcccg tatcctccat
    43741 gaaagatggc gttcatatct aacgccttca cgctcacagc tatttcatct tttttcaatg
    43801 gtgacagatt taacgtcaat aatgacttct ctgtgctgct gttaataatt gcagccccat
    43861 aatcaacagc ctgtaagcgg gagaactctg tgttttttcc gtttatatct aaatatccac
    43921 cccggaatcc ccaggaaaca ttatcaggta taatctgatt atctcctgag agtttaacgc
    43981 gcgagtttcc cccactgatg ttaatggaag aaaaagccgt tgaatcgttg tttttcgctt
    44041 tttgttcaag gattacagta ccagccccaa tcttcagtcc cccttcattt ttccctgtgc
    44101 cattaataat aagcgttccg tccccaactt tatgaaggtc atctccggaa accccgttta
    44161 taccccattt tacagttgta cccgaattga caataatgcc tcctccgaac catgaactgg
    44221 cattgctcgt tgttactgta taatcatccg caaactgaag atatccggct ccctgattaa
    44281 cattatcttt taaatcaata atgccatttg cgcctgtaaa ataaagattt ttacctgcat
    44341 tcagattacc attcaggtta ccatgcatca caaactgttg gtcatcctgc tgaatacggc
    44401 ccacgccttt ggctgcatca tatgtccatg acatatgttc tttactgttt ttattaaatg
    44461 aaataatggg atcgaaatcg gctgtaataa tatttttaat ataatcttca ggtaataaca
    44521 accagttgtt tttttggcca ttaacaccat tattatgcag ggtgactcca gccagagacc
    44581 atttttcact gagagaatca taagcaaata atggcgaccc gctgtcaccc atctgcccat
    44641 aactggctaa tgctccctgt gatttattat aggtgtcacc tggagagctt gatattaaat
    44701 tatttcctga ataccatggg gtgcctactg ttccaccagt taaaaatgtt cccgggatat
    44761 gagtctgttt acctgagcgt tctttaatat actgtgtgcc agcgcctgct ctgtaaaaag
    44821 cagtatactg tgacgaatca ctaagttgtc cggatatatt tatatcagca ggtgcagcct
    44881 ctgtgatgag tttgctgaac cgatgcagag aaaaatcaag gcccgaatgt ttgttgttct
    44941 ttactacagt ataattattt ttatcatagc caaaactggc attgtttaca tcaccaaggc
    45001 ctccgtgttt tgctgataca ccatatccct gcgacaacag ggtcagtgag ccccgccgag
    45061 acacactgct gaagtcaatc attggcacat caagcgtacc aactgcattt ccattattat
    45121 cttttatttc aattcctgtc actccggggg taaacacacc tttattttca gcaaagtcac
    45181 gaaaagtctg atatggtatt tctgcgctga ccgttgatgc tttacccggt aaagcagaaa
    45241 gaatcattga taataccagg gaaaccgtca ttaatcttct tccggttttt cccgaaactt
    45301 tcttacttaa ttctgacaca gctataagcc cacc
//
LOCUS       DAZKHK010000021        44855 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKHK010000021 DAZKHK010000000
VERSION     DAZKHK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.021"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            140..784
                     /gene="adk"
                     /locus_tag="ACHOS5_001230"
     CDS             140..784
                     /gene="adk"
                     /locus_tag="ACHOS5_001230"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9776074.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            916..1878
                     /gene="hemH"
                     /locus_tag="ACHOS5_001231"
     CDS             916..1878
                     /gene="hemH"
                     /locus_tag="ACHOS5_001231"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP9776075.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(1853..2821)
                     /gene="aes"
                     /locus_tag="ACHOS5_001232"
     CDS             complement(1853..2821)
                     /gene="aes"
                     /locus_tag="ACHOS5_001232"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP9776076.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDRPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYSGMLHAFLHYSRMMKTADDALRDGARFFSDRLQQD"
     gene            2973..4277
                     /gene="gsk"
                     /locus_tag="ACHOS5_001233"
     CDS             2973..4277
                     /gene="gsk"
                     /locus_tag="ACHOS5_001233"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP9776077.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(4407..6083)
                     /gene="ybaL"
                     /locus_tag="ACHOS5_001234"
     CDS             complement(4407..6083)
                     /gene="ybaL"
                     /locus_tag="ACHOS5_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP9776078.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(6322..7542)
                     /gene="fsr"
                     /locus_tag="ACHOS5_001235"
     CDS             complement(6322..7542)
                     /gene="fsr"
                     /locus_tag="ACHOS5_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP9776079.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            7760..9412
                     /gene="ushA"
                     /locus_tag="ACHOS5_001236"
     CDS             7760..9412
                     /gene="ushA"
                     /locus_tag="ACHOS5_001236"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9776080.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(9450..9953)
                     /locus_tag="ACHOS5_001237"
     CDS             complement(9450..9953)
                     /locus_tag="ACHOS5_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776081.1"
                     /translation="MKFDHLVDVSFHNFVFNGKFDFVELGQTMAWLEQNFVEPDSKND
                     MGNGFYIWLFGNVEFHFENNKLYMIWCDYLSQIHLGKAIRFDKGILNNVAELNVTRVF
                     NMLVNEGADLQIRRQSPHTLLIHVNRSGVTLWFEADEEQPGDPQNYMLMAFGLTHRDY
                     DARFQYS"
     gene            complement(9950..10606)
                     /locus_tag="ACHOS5_001238"
     CDS             complement(9950..10606)
                     /locus_tag="ACHOS5_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776082.1"
                     /translation="MLKAQLDEFVVGMHSADPVIRIARLISLEEINRHQDFFEHCAKE
                     YRKLATELIFALAEQLNVEMAENNPLVTFAPFKCNRKRKGKMGKWQYCFHGFHCTFEN
                     KKTEQNIEVPLAYGFDFGDLDPYFFSGFIKSIPAWQPLPVAIYDDYHDGSRIIQQLLA
                     LGKLQKIPSPIPQYTGVAAVDRSNVDIANFRSTLESRLHRCKLRWLLKHVKNSQRSER
                     "
     gene            complement(10774..11253)
                     /gene="ybaK"
                     /locus_tag="ACHOS5_001239"
     CDS             complement(10774..11253)
                     /gene="ybaK"
                     /locus_tag="ACHOS5_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP9776083.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(11457..12251)
                     /gene="ybaP"
                     /locus_tag="ACHOS5_001240"
     CDS             complement(11457..12251)
                     /gene="ybaP"
                     /locus_tag="ACHOS5_001240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP9776084.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AEKQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            12384..12686
                     /locus_tag="ACHOS5_001241"
     CDS             12384..12686
                     /locus_tag="ACHOS5_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9776085.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            12722..13063
                     /gene="ybaQ"
                     /locus_tag="ACHOS5_001242"
     CDS             12722..13063
                     /gene="ybaQ"
                     /locus_tag="ACHOS5_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9776086.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(13121..15625)
                     /gene="copA"
                     /locus_tag="ACHOS5_001243"
     CDS             complement(13121..15625)
                     /gene="copA"
                     /locus_tag="ACHOS5_001243"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP9776087.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALVVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVNTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            15887..16819
                     /gene="glsA"
                     /locus_tag="ACHOS5_001244"
     CDS             15887..16819
                     /gene="glsA"
                     /locus_tag="ACHOS5_001244"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9776088.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVS
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            16822..18114
                     /gene="ybaT"
                     /locus_tag="ACHOS5_001245"
     CDS             16822..18114
                     /gene="ybaT"
                     /locus_tag="ACHOS5_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9776089.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            18145..18242
                     /locus_tag="ACHOS5_001246"
                     /pseudo
     CDS             18145..18242
                     /locus_tag="ACHOS5_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18239..18646
                     /gene="cueR"
                     /locus_tag="ACHOS5_001247"
     CDS             18239..18646
                     /gene="cueR"
                     /locus_tag="ACHOS5_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP9776090.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(18647..19870)
                     /locus_tag="ACHOS5_001248"
     CDS             complement(18647..19870)
                     /locus_tag="ACHOS5_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776091.1"
                     /translation="MFSPFRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVIYDPHSHGL
                     IHSLCWQDVPLIRFWSEAGCPTCAEFVYSGFAEDEQGAARFLSALENWNRPWSGITDA
                     FAALTPLFTCMADGYYLLEDRELYPTDGNGHFFWAATDHSSSNPATVAVWDPEYCSFS
                     DTAPCFLLPGQPPSHFNPERATFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATHFNDEQQTLVFPGGECLHKTELQYHIPKLTACKTLPPSAWESFGP
                     DKHISPSETWSEELKQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADII
                     LQALFSTHSPLFIDFARFVISHPAYAIYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKP"
     gene            complement(19989..20447)
                     /gene="ybbJ"
                     /locus_tag="ACHOS5_001249"
     CDS             complement(19989..20447)
                     /gene="ybbJ"
                     /locus_tag="ACHOS5_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9776092.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(20444..21361)
                     /gene="qmcA"
                     /locus_tag="ACHOS5_001250"
     CDS             complement(20444..21361)
                     /gene="qmcA"
                     /locus_tag="ACHOS5_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9776093.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            21507..22184
                     /gene="fetA"
                     /locus_tag="ACHOS5_001251"
     CDS             21507..22184
                     /gene="fetA"
                     /locus_tag="ACHOS5_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP9776094.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            22171..22950
                     /gene="fetB"
                     /locus_tag="ACHOS5_001252"
     CDS             22171..22950
                     /gene="fetB"
                     /locus_tag="ACHOS5_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP9776095.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(23013..23867)
                     /gene="cnoX"
                     /locus_tag="ACHOS5_001253"
     CDS             complement(23013..23867)
                     /gene="cnoX"
                     /locus_tag="ACHOS5_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP9776096.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LENLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RSLLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(23928..24737)
                     /gene="ybbO"
                     /locus_tag="ACHOS5_001254"
     CDS             complement(23928..24737)
                     /gene="ybbO"
                     /locus_tag="ACHOS5_001254"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9776097.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDLPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(24727..25350)
                     /gene="tesA"
                     /locus_tag="ACHOS5_001255"
     CDS             complement(24727..25350)
                     /gene="tesA"
                     /locus_tag="ACHOS5_001255"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP9776098.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQLLVNHDS"
     gene            25321..26007
                     /gene="ybbA"
                     /locus_tag="ACHOS5_001256"
     CDS             25321..26007
                     /gene="ybbA"
                     /locus_tag="ACHOS5_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP9776099.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            26004..28418
                     /gene="ybbP"
                     /locus_tag="ACHOS5_001257"
     CDS             26004..28418
                     /gene="ybbP"
                     /locus_tag="ACHOS5_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP9776100.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLVLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLAFLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSFCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(28659..29753)
                     /gene="mnmH"
                     /locus_tag="ACHOS5_001258"
     CDS             complement(28659..29753)
                     /gene="mnmH"
                     /locus_tag="ACHOS5_001258"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP9776101.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDAHQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQATITVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVVEWVKAQ"
     gene            complement(29822..30748)
                     /gene="allS"
                     /locus_tag="ACHOS5_001259"
     CDS             complement(29822..30748)
                     /gene="allS"
                     /locus_tag="ACHOS5_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP9776102.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            30978..31460
                     /gene="allA"
                     /locus_tag="ACHOS5_001260"
     CDS             30978..31460
                     /gene="allA"
                     /locus_tag="ACHOS5_001260"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP9776103.1"
                     /translation="MKLQVLPLSQEAFCAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            31538..32353
                     /gene="allR"
                     /locus_tag="ACHOS5_001261"
     CDS             31538..32353
                     /gene="allR"
                     /locus_tag="ACHOS5_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP9776104.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            32443..34224
                     /gene="gcl"
                     /locus_tag="ACHOS5_001262"
     CDS             32443..34224
                     /gene="gcl"
                     /locus_tag="ACHOS5_001262"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP9776105.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDITDNAADAPTETCFMHYE"
     gene            34237..35013
                     /gene="hyi"
                     /locus_tag="ACHOS5_001263"
     CDS             34237..35013
                     /gene="hyi"
                     /locus_tag="ACHOS5_001263"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP9776106.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYACALGNKKI
                     NCLVGKTPTGFSSEQIHTTLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLGWMDPYR"
     gene            35113..35991
                     /gene="glxR"
                     /locus_tag="ACHOS5_001264"
     CDS             35113..35991
                     /gene="glxR"
                     /locus_tag="ACHOS5_001264"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9776107.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            36161..37615
                     /gene="ybbW"
                     /locus_tag="ACHOS5_001265"
     CDS             36161..37615
                     /gene="ybbW"
                     /locus_tag="ACHOS5_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP9776108.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            37675..39036
                     /gene="allB"
                     /locus_tag="ACHOS5_001266"
     CDS             37675..39036
                     /gene="allB"
                     /locus_tag="ACHOS5_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP9776109.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            39092..40393
                     /gene="ybbY"
                     /locus_tag="ACHOS5_001267"
     CDS             39092..40393
                     /gene="ybbY"
                     /locus_tag="ACHOS5_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP9776110.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVIYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            40415..41560
                     /gene="glxK"
                     /locus_tag="ACHOS5_001268"
     CDS             40415..41560
                     /gene="glxK"
                     /locus_tag="ACHOS5_001268"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP9776111.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVKAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQA
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(41689..42474)
                     /gene="allE"
                     /locus_tag="ACHOS5_001269"
     CDS             complement(41689..42474)
                     /gene="allE"
                     /locus_tag="ACHOS5_001269"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP9776112.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITTKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(42485..43720)
                     /gene="allC"
                     /locus_tag="ACHOS5_001270"
     CDS             complement(42485..43720)
                     /gene="allC"
                     /locus_tag="ACHOS5_001270"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP9776113.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(43742..44791)
                     /gene="allD"
                     /locus_tag="ACHOS5_001271"
     CDS             complement(43742..44791)
                     /gene="allD"
                     /locus_tag="ACHOS5_001271"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP9776114.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
BASE COUNT        10710 a        11332 c        11836 g        10977 t
ORIGIN      
        1 ttcttcatct gcactttccg caaattatct tgccattaac cgtttcagcc tcaggtgcct
       61 ttcttgaggc aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa attcgacaca
      121 ttttaagggg attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg
      181 gactcaggct cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat
      241 gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga
      301 tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg gttaaagagc gcattgctca
      361 ggaagactgc cgcaacggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga
      421 cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg gaattcgacg taccggacga
      481 actgattgtt gaccgtatcg taggccgccg cgttcacgcg ccgtctggtc gtgtttatca
      541 cgttaaattc aatccgccga aagtagaagg caaagacgac gttaccggtg aagagctgac
      601 tacccgtaaa gacgatcagg aagagaccgt acgtaaacgt ctggttgaat accatcagat
      661 gactgcaccg ctgatcggct actactccaa agaagcggaa gcgggtaaca ccaaatacgc
      721 gaaagttgac ggcaccaagc cggtggctga agttcgcgca gctctggaaa aaattctcgg
      781 ctaatcaagc cgccgcagat ctgctctcgg gcaggtctgc tcatcctcct caggcaatac
      841 ctcttacagc aattagttct tcttcttcac ttttccgcta caattatcaa caagttgaat
      901 cgataagagg cggtaatgcg tcaaactaaa accggtatcc tgctggcaaa cctgggtacg
      961 cctgatgccc ccacacctga agcggtaaaa cgctatctga aacaattttt aagcgacaga
     1021 cgcgtggttg atacctcacg gttgttatgg tggccgttgc tgcgcggcgt gattttgccg
     1081 ctgcgttcgc cgcgtgtggc gaagctgtat gcctctgtct ggatggaagg tggctcgccg
     1141 ctgatggttt acagccgcca gcaacagcag gcgctggcgc aacgtttacc ggagacgccc
     1201 gtggcgctgg gaatgagcta cggctcgcca tcactggaaa gcgccgtaga tgaacttctg
     1261 gcagagcatg tagatcatat tgtggtgctg ccgctttatc cgcaatactc ctgttcaacg
     1321 gtcggtgcgg tatgggatga actggcacgc attctggcgc gcaaacgtag cattccgggg
     1381 atatcgttta ttcgtgatta cgccgataac cacgattata ttaatgcact ggcgaacagc
     1441 gtacgtgctt cttttgccaa acatggcgaa ccggatctgc tgttgctctc ttatcatggc
     1501 attccccagc gttatgcaga tgaaggcgat gattacccgc aacgttgccg cacaacaact
     1561 cgcgaactcg cttccgcact ggagatggca ccggaaaaag tgatgatgac ctttcagtcg
     1621 cgctttggtc gggaaccctg gctgatgcct tataccgacg aaacgctgaa aatgctcgga
     1681 gaaaaaggcg tgggtcatat tcaggtgatg tgcccgggct ttgctgcgga ttgtctggag
     1741 acgctggaag agattgccga gcaaaaccgt gaagtcttcc tcggcgctgg cgggaaaaaa
     1801 tacgaatata ttccggcgct taatgctaca ccggagcata tcgaaatgat ggctaatctt
     1861 gttgcagcct atcgctaaag aatcgagcac cgtcgcgtag agcatcgtcg gcggttttca
     1921 tcatccgtga ataatgcaaa aaggcgtgca gcataccgga gtagagtttg aactcacaag
     1981 gttgctggtg tgccgctaac gtctggtaaa gcagacggct gtcatccagc agtggatcga
     2041 actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacaggc
     2101 agtaatatgg cgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca
     2161 aatcctgttg cgttaagcca tcccagacac cacccaacag acgacgagtc acggaatccc
     2221 gtaatccgta aagcccatac cacagcaaaa cgcccgcaac tttaccgcaa tcgatctgtt
     2281 tatcgcgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccagcaa
     2341 aaccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacagg
     2401 cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg gtgtaatcaa
     2461 tgccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat
     2521 cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc ggcctatctg
     2581 gctgcggata aaagatacgt gttttcacct gcccatattt tgttggaacc ctgtaagctc
     2641 tggttgccat ttctggagcg cccacattcc agaatcggcg ctcaagcgtg taatactgtc
     2701 gttgctcagc aatcgttccc gttgcaggcc agggcggtaa atccggctga agagtattca
     2761 caacggtctt catttcagca gagataaggt ccagaacggg taatttgttt tccggcttca
     2821 tgcaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc
     2881 cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt
     2941 cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca
     3001 aacattactt ccccgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg
     3061 aaaccagcgc cgcctgggta gtgggtatcg atcaaacgct ggtcgatatt gaagcgaaag
     3121 tggatgatga attcattgag cgttatggat taagcgccgg gcattcattg gtgattgagg
     3181 atgacgttgc cgaagcgctt tatcaggaac taaaacagaa aaacctgatt acccatcagt
     3241 ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg gacgaccgtt
     3301 cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagctatgcc tatcgttacc
     3361 tgtgtaacac ctccagccgt accgatctta actatctaca aggcgtggat ggcccaattg
     3421 gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agccccggcc
     3481 acatgaacca gctgcgggct gaaagtattc cggaagatgt gattgccgga gcctcggcac
     3541 tggttctcac ctcttatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca
     3601 tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca
     3661 agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtctcta
     3721 tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg
     3781 catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct
     3841 tgtatatggc aggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc
     3901 cgggtgctat agcggaattt aaccagtatg agtttagccg cgccatgcgc cacaaggatt
     3961 gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa
     4021 tcatgaacac taatggagca ggggatggcg cactggcagc gttgctgcat gacattaccg
     4081 ccaacagcta ccaccgtagc aacgtaccaa actccagcaa acataaattc acctggttaa
     4141 cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga
     4201 accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt
     4261 cttactggga tcgttaagct atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat
     4321 ctggcatttt gctctcaatg cctgatgcga cgctggcgcg tcttatcagg cctacatatc
     4381 cgcgcctatt atccggcaac caccgtttac cccgtcacca cctcacccgc cggtggcgtt
     4441 tccagcagtt ccagcatagt acgggcaatt tcacgctcac ccatcactac ctgattcgca
     4501 ccacgttcgg tgatatacgc cacttcatcg tcataatggg cgcgggcaat aatctcaata
     4561 tccggatttt tcgcgcgggc agatgccaca atctcgcccg cttcatagcc gttgggaatc
     4621 gtcaggatca gccattttgc acattccaga tgcgccagtt gcataatttc ttcgttcgcc
     4681 gcattgccca atactgcgcg gaccccgcgc tcgcgcagct catcaacacg ggttcgtgac
     4741 gtctcaatca ccaccagcgg aatatcagag gcgagcaatt tctcccccag caggctgcct
     4801 acacgaccgt aacccaccag tagcgcatgg ttgcaaatat ccactgggat ctgcttctct
     4861 tcttcgattg cctcttccag cgtctgctct tccagcgttt cggtcttcgc cagatatttt
     4921 tccagtagtg cgaacagtac cgggttgagc ataatcgaca ggatcgcccc tgccagtacc
     4981 aggttttgtc cggcctgcgg cagtaaattt aatgccattc ctagtcccgc caggataaac
     5041 gcgaactcac caatctgcgc caggctggcg gcgatggtta acgccgtgcg ctgggagtga
     5101 ccaaacaaac gcacaaggaa cagggccgcg accgatttac caaacagaat aattgccagc
     5161 gtcgccagta ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa catcccgacg
     5221 gagacaaaaa acagcaccgc aaacgcgtcg cgcaatggca gtgtatcgtg ggcggcacgg
     5281 tgactcagtt cagactcgtt cagtaccatc ccggcaaaga acgcaccgag tgcaaaggag
     5341 acatcaaaca gctctaccgc accaaaggca atccctaacg ccagcgccag caccgacagg
     5401 gtaaacagct cgcgggaacc ggttgctgcg ctgcgtgcca taatccacgg caccagacgg
     5461 cgacctacca gcatcataat ggcgataaat gcgatcacct tgccgatggt gatccccata
     5521 tcgactgcaa gagtggcaaa gcccacatcg ccctgttcca tcattcctgc taccgcgggt
     5581 agcaatacca gtgtcaggac catcaccagg tcttccacaa tcaaccaacc gatggcgatt
     5641 tgcccacgct gactgtcaat taattgccgt tcttcaagtg cgcgcagtaa caccacggta
     5701 ctggcggtgg aaagacataa gccgaacacg ataccggtca ttaacgacca gcccagcacg
     5761 gcggagagcg ccatacccag cagcgtcgcc acggctatct gggcgatcgc gccgggaatg
     5821 gcgatggact ttaccgccat caaatccttg agcgaaaaat gcaaaccgac gccgaacatc
     5881 agcaaaataa cgcccagttc agccagttcc ggcgcaagct tggtatcggc aacaaagccc
     5941 ggagtgaatg gtcctgccag cacacccgct aacagatatc ccaccagagg agaaatacgt
     6001 agtttattgg ccagcatgcc gaggataaag gcgagcacaa ggccgccaac aatggtggtg
     6061 ataagcgggg tggcgtgatg cattccgtct ccttttcctg gtggttattg tccatttttg
     6121 gccgggaaaa ccaaaattac aggtaatagt ttatgacaat ttcattgatg atgtttatga
     6181 ataattgttg aattttgcag aaaaatggaa ttagctgcaa aaaaaagcac ggatcggaaa
     6241 acagaaggcg gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt
     6301 ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa
     6361 catccccaat agtggcagga aagcacagat tttatagact aactctatgc tggtgtggtc
     6421 ggcgataagc cccagaactg ccgcccccag acctcccatg ccgaaagcaa aaccgaaaaa
     6481 tagtccagaa accataccga tacgtcccgg aagcagctcc tgggcataga ccagaatggc
     6541 agagaatgcc gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag
     6601 gctggcgtag ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac
     6661 atatttccgt ccaattttat cccctacagg cccgccaatc accgtacctg ccgcaacggc
     6721 aaacaggaag gcaaacaaat gaagctgagc attctggata gataatccga atttttgcat
     6781 cagataaaag gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag
     6841 gattaacaga atgctgaccg ccagaacaac tttattgcgc ggcagtggat tgataatcgt
     6901 cgctttgggt tttcctttat tcattcggtg ctgtgccgag taccaacggc tgatttgcgc
     6961 caacaccacg atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata
     7021 aggcgcgata atcaccgccg ccagcaaggg gcccagggaa ctgccaaagt tgccgccgac
     7081 ctgaaatata gattgcgcca ggccatgacg cccgccggaa gccatacggg ccacgcgaga
     7141 agattccgga tgaaagaccg atgaaccggt accgaccagc gccgctgcca gcagaactgc
     7201 gccgaaactg cccgccagcg ccagcagcac cagaccactt aaggtaaagc acatgccaat
     7261 cggcaacgac cacggcatcg gatatttatc ggtccagtag ccgaccaccg gttgcagtag
     7321 cgaagaggcg agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa
     7381 ttctgactga agcagtggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag
     7441 atgtgagagg ctgatagcac ctaaaatacc aaacgatgtt cgggccttag tcgttgacgc
     7501 agccgcgccc gccacaggct gggtttgttc actcattgcc ataggaaagt cactttttca
     7561 gggttgcgat gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg
     7621 cgccgcctga aggaagtctc aacgcggaat acagaatttc taatctggat gcagatttat
     7681 cttcaccgga cgcagacttg tctatgatgt cgcgtcatac tatttttcaa cacgttgaaa
     7741 tcaggtcagg gagagaagta tgaaattatt acagcggggc gtggcgttag cactgttaac
     7801 cacatttaca ctggcgagtg aaactgctct ggcgtacgag caggataaaa cctacaaaat
     7861 tacagttctg cataccaatg atcatcatgg gcatttttgg cgcaatgaat atggtgaata
     7921 tggtctggcg gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg
     7981 cggtagcgtg ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtccgactt
     8041 acaggatgcc gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat
     8101 cggtaatcat gaatttgata acccgctcac cgtattacgc cagcaggaaa agtgggctaa
     8161 gttcccgttg ctttccgcca atatctacca gaaaagtacc ggcgagcgcc tgtttaaacc
     8221 atgggcgctg tttaagcgtc aggatctgaa aattgccgtt attggcctga cgacggatga
     8281 cacagccaaa attggtaatc cggaatattt cactgatatc gaatttcgta agcccgctga
     8341 tgaagcgaag ctggtgattc aggagctgca acagacagaa aagccagaca ttattatcgc
     8401 ggcgacccat atggggcatt acgacaatgg tgagcacggc tctaacgcac cgggcgatgt
     8461 ggagatggca cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg gtcactcgca
     8521 agatccagtc tgtatggcgg cagaaaacaa aaaacaggtc gattacgtgc cgggtacgcc
     8581 atgcaaaccg gatcaacaaa acggcatctg gattgtgcag gcgcatgagt ggggcaaata
     8641 cgtgggacgg gctgattttg agtttcgtaa tggcgaaatg aaaatggtta actaccagct
     8701 gattccggtg aacctgaaga agaaagtgac ctgggaagac gggaaaagcg agcgcgtgct
     8761 gtacacccct gaaatcgctg aaaaccagca aatgatctcg ctgttatcac cgttccagaa
     8821 caaaggcaaa gcgcagctgg aagtgaaaat aggcgaaacc aatggtcgtc tggaaggcga
     8881 tcgtgacaaa gtacgttttg tacagaccaa tatggggcgg ttgattctgg cagcccaaat
     8941 ggatcgcact ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg attctatcga
     9001 agcaggtgat atcagctata aaaacgtact gaaagtgcag ccattcggca atgtggtggt
     9061 gtatgccgac atgaccggta aagaggtgat tgattacttg accgccgtcg cgcagatgaa
     9121 gccagattca ggtgcctacc cgcaatttgc caacgttagc tttgtggcga aagacggtaa
     9181 actgaacgac ctgaaaatca aaggcgaacc ggtcgatccg gcgaaaactt accgcatggc
     9241 gacattaaac ttcaatgcca ccggcgggga tggctatccg cgccttgata acaaaccggg
     9301 ctatgtgaat accggcttta ttgatgccga agtgctgaaa gcgtatatcc agaaaagctc
     9361 gccgctggat gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt aatgaacccc
     9421 gtcgcggcgt catgatatga cgccgccatt tatgaatatt gaaatcttgc atcgtaatcg
     9481 cgatgtgtca aaccaaatgc catcagcata taattctgcg gatcaccagg ctgttcttca
     9541 tctgcttcaa accaaagtgt cacgcctgag cgattgacgt ggattaagag tgtatggggc
     9601 gattgacggc ggatttgcag atcagcgcct tcgttaacaa gcatgttaaa tacacgcgta
     9661 acgttcaact cagcgacgtt attcaatatt cctttatcaa atcggatcgc tttgcccaaa
     9721 tggatttggc tcagataatc gcaccaaatc atatatagct tgttgttttc gaaatgaaac
     9781 tcaacattac caaatagcca gatataaaaa ccatttccca tatcattttt agagtccggc
     9841 tcaacaaagt tttgttccaa ccatgccatt gtttgcccca gttcgacaaa atcgaatttt
     9901 ccgttaaaga caaaattgtg gaagctaaca tcaacaagat ggtcaaattt catctttcgc
     9961 tcctttgtga attttttaca tgcttcaaaa gccagcgtaa cttacatcgg tgcaggcgac
    10021 tctccagtgt tgagcgaaaa tttgcgatgt cgacattgga ccgatctacc gcagcaaccc
    10081 ccgtgtattg tgggatcggt gaagggattt tttgtaactt tcccagcgcc agcagttgtt
    10141 gaatgatcct gctaccgtcg tggtaatcat cgtaaatagc cactggcaga ggctgccagg
    10201 cggggatcga tttgataaat ccggaaaaaa aatacgggtc cagatcgccg aaatcaaagc
    10261 cgtaggcgag gggaacttca atattttgct ctgttttttt attctcaaat gtacaatgaa
    10321 aaccgtgaaa acaatactgc cactttccca ttttgccttt tcgcttacgg ttgcatttga
    10381 agggggcaaa ggtaactagc gggttatttt ccgccatctc aacgttcagt tgttcagcca
    10441 atgcaaatat aagctctgtc gccagcttac gatattcttt cgcgcagtgt tcgaaaaaat
    10501 cctgatggcg gttaatctct tcaagcgata ttaatctggc gatcctgata accgggtcgg
    10561 cagaatgcat gccaacaaca aattcatcga gctgtgcttt cagcaccagc ccgaattcct
    10621 cacgatacct ctctgcttcg ctggcaaaat gcagatgtaa taccttctct ctcgatgttt
    10681 gccgaagtaa ggcgatatcc ggtatctcat ctgcacaagc gatacttaat gtctctgtaa
    10741 tcctttccac atctcatctt ccgtgtgcat cacttaatcg cgacgggcga tatcagcaaa
    10801 tttggcatcg agtatctttg ccaggtcgct tgccgccagt tcgatatcca gtccgcgctt
    10861 gccgccggaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgttggcag
    10921 acgttttttc tgccccagtg ggctaatccc tccaaccaga tatcccgtcg aacgctgcgc
    10981 gaccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
    11041 atccagttga cctgcgaccg gcgtaacggc cacggcaagg tgtttcatat caccgttcac
    11101 tgcgaccagc agcgttttgt agacctgatc cggattcaaa cctaattttt tgacgacttc
    11161 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggtg tggatttgaa acgaaatctt
    11221 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
    11281 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
    11341 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
    11401 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
    11461 cgcaacattt gcggcaaatt cccttcgcca tacaggtgta gtgcaccaac cgcgaccaca
    11521 tagcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
    11581 agcacatcgt acagcgactg actgaacgta ttgggcaacg ttatttcatt attttgcggc
    11641 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt gtgccagtgg
    11701 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcag catggcaatt
    11761 tgcttttctg cgccctccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
    11821 agctggtaat caataccata ttctgcccgc agccccagtt tttgcgcctg cgtcgcctga
    11881 agaaccatcg cgatttgcca caacggttga gtagaaaaga gcgacgggga aatgcccatc
    11941 tcctggctaa tgtgctgcag gttttggagt tggtcctcgc taatgcgctc ttccagggcc
    12001 tcgcaggcag gcaaattggc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
    12061 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
    12121 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccggggag ggtgatatcg
    12181 atagcaggcc aggagtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
    12241 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcgggg agggatggcg
    12301 caaatggagt tgattgttgc acttgcgcac agtgcttgtg tgcattacaa tgattaaata
    12361 atggacaagg agatgagaga ggaatggctc aaaagaaaaa tataaggagt tttcgtgatg
    12421 cctggttggc tgattttttt gtccattcaa cgccacatcg aaagatccct gctgaaatcc
    12481 ataccacgtt atcacgaaag ttggacatca ttaatgctgc cacttctcat cgggatttac
    12541 gctcccctcc ggggaaccga tacgaagagt tatccgggaa attacaggaa tactcctcca
    12601 ttagagtgaa taaacagtac cgactgattt ttaaatgggt taatggcaag gccgaagaat
    12661 tgtttttaga cccacacaac tactaaaaca tagctgtcgt attgacgaga aaggtaaaac
    12721 tatgaaacag gcaacaagaa aaccgacgac acccggagat attcttctct atgaatatct
    12781 ggaaccgctc gatttgaaaa tcaatgagtt agcagagttg ctgcatgttc atcgtaatag
    12841 cgtcagtgca ctgatcaata acaatcgtaa actcaccact gagatggcat tccgtctggc
    12901 gaaagttttt gataccacag tcgatttttg gctaaacctc caggcggcag ttgatctttg
    12961 ggaagttgaa aacaacatgc gtacccagga agaattggga cggattgaaa cagtggctga
    13021 atatttggca cgccgtgaag agcgtgcaaa aaaggtcgcg tagaataatt tccctataac
    13081 ggatgcgcca ccctaaagca gcgcatccgc aatgatgtac ttattccttc ggtttaaacc
    13141 gcagcaatcg gttggcgtta ctcactacgg tgatcgacga gagcgccatt gccgctccgg
    13201 caaccaccgg gttaagcagt gttccagtga acggccacaa aataccggcg gcgaccggaa
    13261 taccaatgct gttgtagata aacgcaccga gcaggttctg cttcatgttg cgcagcgttg
    13321 cgcgggaaat tgcgagcgca tccgcaacac ccatcaggct atggcgcatc agggtaatcg
    13381 ccgcggtttc aatggcaaca tcactgccgc cacccatcgc aataccgaca tccgcctgag
    13441 ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc cacctgacgt ccttcacttt
    13501 gcagacgttt gatcgcttcg gctttaccgt ctggcagcac gccggctatc acctcatcaa
    13561 tccctgcttc tttagcgatc gcattggcgg tggtcgggtt atccccggtt agcatcacca
    13621 ggcgatatcc cgctttatgc aggcgttgca gcgccgccac gctatcacta cgcaacggat
    13681 cgcgtacagc cagcagggct actgctttcc cgtcaaccgc cagcagcaca ggcgttgccc
    13741 cttgcgatgc ctgagctgaa atatctgctt caattgcttt ggtattgacc tgttggtcat
    13801 ttaacaacgc ctgattgccc agcaataacg catgaccttc agcttcaccg ctcacgccca
    13861 gcccgcgcaa tgtgcggaaa ccgttgacct gcggtagctg catatcacct gctttatcga
    13921 ggatcgcccg tgccagaggg tggctggaac cttgctccag cgccgccgcc agacgcaatg
    13981 cttgcgcttc atcaatatca gcaaatgttt ttactgcgac aacctgcggc ttcccttcgg
    14041 tcagcgtccc ggttttatcg aacactacgg tgtcgagcgt actggcgcgt tgcagcgcgt
    14101 cggcgtcccg taccagcacg ccaaactcag ccgcccgccc gacgccggaa ataatcgaca
    14161 tcggcgtcgc cagtcccagc gcacacggac aggcaataat cagcaccgtg gtggcaatca
    14221 ccagggtata gacaatctgc ggtgccggac caaagaaata ccagattgcc gcactgatca
    14281 gcgcaatcac caccactacc ggcacaaata cggctgagat tttatccgcc agttgaccga
    14341 tttctggctt actgctctgg gcctggcgca ccatacgaat aatccgcgac agcgtagtat
    14401 ggctgccaac cgcactggcg cgaaacagca cgctaccgtc ctgcaccact gtcccggcat
    14461 ggacgctatc gccttcgcct ttttgctgcg ggataggttc gcccgtcagc atcgcttcat
    14521 ccagccacgc ttcgccttga gtaatctcac catctactgg cacacggtcg ccggtcgtca
    14581 ggcgcagtaa catacctggc tgaacatctg ccagcggcac gtttttttca ccttcgtcgg
    14641 tgaccaggcg cgccgtcggt ggggttaaat cgagtaattt ctccagcgcc ttagaagaac
    14701 gctgacgcgc gcgcgcttcc agcatatggc cgagattgat cagaccgata atcatcgcgc
    14761 tggcttcgta ataaagatgt cgcgcttcca tcgggaacca ctgcggccac aggttgacgc
    14821 tcatcgaata gagccacgcc acgccagtac ccagcgccac cagcgtatcc atcgtcgctg
    14881 caccgttcag caggcttttc catgcactgc ggtaaaaatg gccgccggcg aaaaccatca
    14941 ctgccagggt tatcaggccg ataaccaacc acaggctgcg gttgtcagcg gtgaccatca
    15001 tgttatcgcc gatcatcccc cagaccatca ccgggatacc caccaccagc gcgacaattg
    15061 cctgccagcg gaagcgcttc atcgtagcga cggcggtttc ttgctggcgc tcgcggcgtt
    15121 tagcgtcatc ttcaatcgct tccgcgccgt agcccgcttt ttccaccgcc tgcactaaat
    15181 cttgtgggga ggcactgccc atcaccagcg cagtacgctc cgccaggttt acccgtgcct
    15241 gagtgacgcc cggtacgctt tgcaacgcat tttgtacgcg ggtgacacag ctggcgcagc
    15301 tcatgccgct cagcagcaac tgctggctgt catcgtcatc ggcggtcgct gccggaagcg
    15361 cctcagaaac cgctgtcagt gcttccgacg ggattgatga ctccgccagc ggtttagcct
    15421 ttgggtggct tacagatgcg tcataacccg cttgtttgat ggtttcgatc agctgttctg
    15481 cactggcagt cccggtaacg tgcgcttcag tgatagacac atccgcctgt tcaacatccg
    15541 ggcgctgttc gagactttct ttcacgcgtt taacgcagtg accgcaggac aggccgtcca
    15601 gggtcaggtc gatagtttgt gacataaaac actcctttaa gacagttttg actggctgtg
    15661 ataaaggtta aaccttccag caaggggaag gtcaagaaat taataaacca ggcgggtaaa
    15721 agtccgtaaa gattaaaaaa tcggctcgat ttgcatcagg attagacatt tatctctttg
    15781 ttttcctgta gttaagttgc gggtgctaag ttaaatcagg atgcctgaaa atcggcaccg
    15841 gggtgaggaa ttacctcccg catctataaa aaggagttaa caaaagatgt tagatgcaaa
    15901 caaattacag caggcagtgg atcaggctta cacccaattt cactcactta acggcggaca
    15961 aaatgccgat tacattccct ttctggcgaa tgtccctggt caactggcgg cagtgtctat
    16021 cgtgaccagc gatggcaacg tctatagtgc gggtgacagt gattaccgct ttgcgctgga
    16081 atccatctcg aaagtctgta cgttagccct tgcgttagaa gatgtcggcc cgcaggcggt
    16141 acaggacaaa gttggcgctg acccgaccgg attgcccttt aactcagtta tcgccttaga
    16201 gttgcatggc ggcaaaccgc tttcgccact ggtaaatgct ggcgctattg ccaccaccag
    16261 cctgattaac gctgaaaata ctgaacaacg ctggcagcga attttacata tccaacaaca
    16321 actggctggt gagcaggtag cgctctctga tgaagtcaac cagtcggaac aaacaaccaa
    16381 cttccataac cgggccattg cctggctgct gtactccgcc ggatatctct attgtgatgc
    16441 aatggaagcc tgtgacgtgt acacccgtca gtgctccacg ctcatcaata ctattgaact
    16501 ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat ccgctgacgc ataaacgcgt
    16561 tcttcaggcc gacaatgtgc cgtacattct ggccgaaatg atgatggaag ggctgtatgg
    16621 tcgctccggt gactgggcgt atcgcgttgg tttaccgggc aaaagcggtg taggtggcgg
    16681 tattctggcg gtcgttcctg gcgtgatggg aattgccgcg ttttcaccac cgctggacga
    16741 agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg gtcgctaagc aactcggcta
    16801 taacgtgttt aagggctgat catgatgaac acggaaggta ataacggtaa caaacctctc
    16861 ggtctatgga acgtcgtttc catcggcatt ggggcaatgg tgggggcggg gatcttcgcg
    16921 ctgctggggc aggctgcgtt gctaatggaa gcctcgacct gggtcgcctt tgcttttggc
    16981 ggtattgtgg cgatgttttc cggttatgcc tatgcgcgtc tgggggcgag ctatcccagc
    17041 aatggcggta ttatcgactt ctttcgtcgc ggattaggca acggcgtttt ttcgctggcg
    17101 ctctcgttat tgtacctgtt gacgctggcg gtgagtatcg ccatggtcgc tcgtgctttt
    17161 ggcgcttatg ccgtgcagtt tttgcatgaa ggcagccagg aggagcacct tattttgctc
    17221 tacgcgttgg ggatcattgc ggtgatgacg cttttcaact ccttaagcaa ccatgcggta
    17281 gggcggctgg aagtgatcct cgtcggcatt aaaatgatga tcctgttatt gctgatcatt
    17341 gccggtgtct ggtcgctgca accggcgcat atttccgtct ctgcgccccc cagctccggt
    17401 gcgttcttct cctgtatcgg gataaccttc cttgcctatg cgggctttgg catgatggcg
    17461 aacgcggcgg ataaagtgaa agatccgcag gtcattatgc cacgggcgtt tctggtggcg
    17521 attggcgtta ccacgttgct ttatatctcg ctggcactgg ttttgcttag cgatgtatcg
    17581 gcattagagt tagaaaaata tgccgatacc gccgtagcgc aggctgcttc tccgctcctc
    17641 gggcatgtgg ggtatgtgat cgtcgtcatc ggcgctttac tggcgacggc ttcagccatc
    17701 aacgcaaacc tgttcgccgt gtttaacatt atggacaaca tgggcagcga acgcgaactg
    17761 ccgaagctaa tgaataaacc tctgtggcgg cagagtacct ggggcaacat catcgtcgtg
    17821 gtgttgatta tgctgatgac ggcggcgctg aatttaggct cactcgccag cgttgccagc
    17881 gccacctttt tgatctgcta tctggcggtg tttgtggtgg cgatccgcct gcgtcatgat
    17941 attcacgcct cgttgccgat tcttatcgtt ggtacgttgg ttatgttgtt ggtgatcgtt
    18001 ggctttatct acagtctctg gtcccagggc agccgtgcgc tgatatggat tattggcgca
    18061 ctcttactca gccttattgt ggcaatggtc atgaagcgca ataaaactgt ataacatctc
    18121 tctgtgcgca gtacctcctg tattattgtg gtggcggtcg atattcgcac tggcaaaaaa
    18181 acgtgcttga atatctgttg aaacccttta acaaagcaca ggaggcgttg cgcgaacgat
    18241 gaacattagc gatgtagcaa aaattaccgg cctgaccagc aaagccattc gcttctatga
    18301 agagaagggg ctggtgacgc cgccgatgcg cagcgaaaac ggttatcgca cctacacgca
    18361 gcagcatctc aacgaactga ccttactgcg ccaggcacgc caggtgggct ttaacctgga
    18421 ggagagcggc gaactggtga atctgtttaa cgatccgcag cgtcacagcg ccgacgtcaa
    18481 acgtcgtacg ctggagaaag tagcggagat cgaacagcat atcgaagaac tgcaatccat
    18541 gcgtaaccaa ctgctggcgc tagcgaatgc ctgccccggt gatgacagcg ccgactgccc
    18601 gattatcgaa aatctttccg gctgctgtca tcatcgggcg gggtgattaa ggcttgcgaa
    18661 aatagtcgtc cattattttc gtcaactccg gacgttcccc gtcatcattg atggcaaaat
    18721 ccagaaaaaa agcatctgct tgcggcgttc ggttttgcgc catcaaccgg aaggtgaggg
    18781 ggcgataaat ggcgtatgcc ggatggctga taacgaatcg ggcaaagtca ataaaaagcg
    18841 gcgagtgtgt ggaaaacagc gcctgcaaaa taatatcagc cttttcatcc tcccccaaac
    18901 cttgttggat catgctctta atacgagttt cgctcaaatt cccggcctca acaatttgcc
    18961 aggcttgatc cagcgagggg taccgactga cgctctgctt taactcttct gaccaggttt
    19021 cggatgggct gatgtgctta tcagggccga aactctccca tgcgctgggc ggtaatgttt
    19081 tgcacgcggt gagttttggt atgtggtatt gcagctctgt tttgtgcaga cattccccgc
    19141 caggaaatac cagggtttgt tgctcatcat taaaatgggt tgccgtgctg agcaccagtg
    19201 ttttgagcgg tcgcccttcc agcgcggcgg cagtagcctt atggtgtccg tcgagcagaa
    19261 cgcagagata ggaatcaggg agataccagg ccagagcgcg ggcgtccggt ttatcgcgat
    19321 aaaacgtcgc acgctcagga ttaaaatgtg atggcggctg gccgggtaga agaaagcagg
    19381 gcgcggtgtc actgaaagag caatattcag gatcccagac ggcaacggtt gccggattac
    19441 tggaactatg gtccgttgcc gcccaaaaga aatgcccgtt gccatccgtc gggtaaagtt
    19501 cacgatcttc cagcaaataa taaccatcag ccatgcatgt gaacagcggt gtcagcgcgg
    19561 caaaagcgtc cgttatgcca gaccacgggc gattccagtt ttcgagagca gacaaaaacc
    19621 gggcggctcc ctgttcatcc tccgcaaaac cgctgtagac aaattccgca caggtagggc
    19681 atccagcctc ggaccagaaa cgtattaacg ggacatcctg ccagcagagc gaatggataa
    19741 gaccgtgcga gtgcggatcg taaatcacac attgacgcag aaatcctttc ccggacttca
    19801 ccgaaaaaat gacgcccgca gcggtatttt tcagcagacg tacttcaggt tttcgaaagg
    19861 gggaaaacat ggctactcct tagccgaaca ttcaattaac actatgtagg gggctatatg
    19921 ccggatgcgg cgtaaacgcc ttatccggca tggggtttgg tacagatttc caggtgtgat
    19981 aagacgcgtc aggcgatgac cgcacggata atcagcgtta ttccttctat cgcaatcacc
    20041 tcaacatgcg taccagcgcc gagatcctcg ctggcgctga caggccatga actgtcaccg
    20101 acgcgcatat gaccacgccc gttgaccagc ggagattcca gaacaaaacg tcggccaatc
    20161 aactgctgcc cgcgctggtt taaatgactg tcgctgtgct tttgttcgcg cacccgccgc
    20221 gacaaccatt tccaccacag ccaggcggcg agcagcgtca ggacggcaaa cattacccct
    20281 tgccactccc aacccagcgg cagcaaccag accaccagac cggtaatcac tgctgccacg
    20341 ccgctccaca acagataacc attgccgccc aacatctcgg ctgccagcag caaaccgccg
    20401 agacttagcc agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt
    20461 tgttggcgct gtctttcacc agctcagcaa tcccggcaat cgaccccatc aggctgctgg
    20521 cctctaatgg catcatcacc actttgctgt tactggacga accgatctgc tgtaacgctt
    20581 cggtgtattt ctgtgcgacg aagtagttca ccgcctgaat atcaccggag gcgatggctt
    20641 cagacaccat tttagtggcg cgggcttctg cttcagcgga acgttcacgc gcttcagcct
    20701 gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt
    20761 cggctttgag gatttccgcc tgacggatcc cttccgcttc aaggatgtaa gcgcgtttgg
    20821 tacgttccgc tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacat
    20881 cacgaatttc gatacgggtg actttaatcc cccacgggtt ggtggcctca tcgacaatat
    20941 gcagcagacg cgagttaatg ctgtcgcgct gagagagcat ttcatcaagt tccattgaac
    21001 ccagcacggt acgaatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga
    21061 cttcataagc cgcgcgcggc gcgtcaatca cctgaataaa gcacacagcg tcgatggtaa
    21121 cgttggcgtt atctttcgag ataacttcct gggaagggat gtcgagcact tgctccatca
    21181 tattgatctt gcgaccaatg cgatccataa acggcaccac caggcttaga cctggctgta
    21241 acgttttggt atagcgacca aagcgttcta ccgtccattg atagccctgc ggcacgattt
    21301 tgacgcccgc gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca
    21361 tcggaaaaac ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa
    21421 ttatacctga ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta
    21481 gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa
    21541 acgtaggata tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg
    21601 ctggcgaatt taagttaatt accggtcctt ctggttgtgg caaaagtaca ctgctaaaaa
    21661 tagttgcttc attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca
    21721 gcacactaaa gccagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc
    21781 tgtttggcga tacggtatac gataacctga tttttccctg gcagatccgt aaccagcagc
    21841 ctgacccggc catttttctc gattttcttg aacgcttcgc tttaccggac agcattttga
    21901 cgaagaatat cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc
    21961 tgcaatttat gccgaaggtt ttattgctcg acgaaatcac cagtgcgctg gatgaaagta
    22021 ataaacataa cgttaatgag atgatccacc gttatgtgcg cgagcaaaat attgccgtgc
    22081 tgtgggtgac acacgataaa gacgaaatta atcatgcgga taaggtgatt acactgcaac
    22141 cgcatgctgg agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca
    22201 ttggcactgg cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaagctg
    22261 gcgctggaga aagacattct ctggagcgtc gggcgcgcga taattcagct gattattgtc
    22321 ggctatgtgc tgaagtatat tttcagcgtg gatgatgcaa gcctgacatt attgatggtg
    22381 ttatttatct gctttaatgc ggcgtggaat gcgcaaaaac gcagtaaata tattgctaaa
    22441 gcttttatct catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg
    22501 attctctcag ggtcgattga atttatcccg atgcaggtga tccctatcgc cgggatgata
    22561 gccggtaacg ccatggtagc ggtggggttg tgttacaaca atttagggca acgggttatt
    22621 agcgaacagc aacagattca ggagaaactg agtcttggcg cgacgccgaa acaggcttca
    22681 gcgatattga ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa
    22741 acggtaggct tagtgagttt acctggaatg atgtccgggc tgatatttgc cgggattgat
    22801 ccggtgaagg cgattaaata tcagattatg gtgaccttta tgctgctctc aactgccagc
    22861 ctgtcgacca ttattgcctg ctatttaacc tatcgtaaat tttataattc gcgccaccaa
    22921 ttggtggtga cgcaattgaa gaagaaatga tgcaatgccg gatgcgcagc atcgcatccg
    22981 gcgttgtggt tcatgtgccg atcaacggaa actcaataca acaacgcata cagttggcgg
    23041 cgatacttcg acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt
    23101 ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc
    23161 agcgcctctt cattgcgccc aacctggtgc agttgcagtg ccagttgtgt cgccagtgcg
    23221 gcatcttctg gattctccgc cacctgctgt tgcaactgtt gaatttccgg cgtatctgcc
    23281 gcctgcttca gcagttcgat ttgcgccacc agcccctggt agcgggtgtc ctgatcctgt
    23341 aacggaatgg ttttcagcac ggcttctgca tcttcagaac ggttcagcgc aatcagcgtt
    23401 tctgccagca gcaggccgat ttccccgttc tgattcgaca actgccaggc gtctttcagc
    23461 aatggcaggg cgtcggtgta attgccttcc tgcatcagct gcatcgcctg ctgcgctttc
    23521 agctcttctt cgcgcggcag caccttatcc agcagggagc ggatcgcctc ttccggttgc
    23581 ggcccctgga agccgtctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg
    23641 cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata
    23701 aattgcccgt tgtactgcgc cgcaaggttt tccagaattg gggttaactg caaacagtgc
    23761 tggctacgtt cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc
    23821 tgttgcaggt tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct
    23881 gttgtcgata ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat
    23941 attttgtcca tcacgcgccc cggcagcagg cgcttaagca ccattactgc ccaggtcacc
    24001 agcgttaccg gatagcgcat cttcggcttc tcgctaataa aagcatggcg tactttgtcg
    24061 accaccgctt ccggccccaa cgtaaagcgg gcggcgatgc cgggattttc gaccggttta
    24121 tcgctttgtg tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc
    24181 aggctaactt taattccgct gtggcgtagc tccatgcgca gcgcatccga ccacgcctcc
    24241 agcgcatatt tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccattacc
    24301 gatgaggtca tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg
    24361 gtgagctggt gtgcgccgaa aaagttggca gaaaactgct gttccatctg cgcgcggctg
    24421 atggtggaaa gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa
    24481 ttatcggtca gggcgatcac ttcgtcggct gcgcgatcaa cactttctgg caaatccaga
    24541 tcgatcaaca cgccggtgaa tcccatattg ttcatgcgct caacatcatc cggtttccgg
    24601 caaccggcca gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt
    24661 ccactggaac atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc
    24721 gttgctttat gagtcatgat ttactaaaag ctgcaactgc ttcgccatcc agtcggcaat
    24781 aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag
    24841 gtagacctct tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg
    24901 gtaaatggcg ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg
    24961 cattaacaat ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg
    25021 ctcggtttgt tgtggctgaa aaccacgcaa accgtcattg ccgcccagtt caaccagcac
    25081 ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc gccagccctt gttgcgaggt
    25141 gtcaccgctg atgctggcat tgactaccga cgttttactc tgccacttat cattcaacaa
    25201 ggcaggccag gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat
    25261 caataacgtg tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa
    25321 atgccagcgg aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag
    25381 catgaactct ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca
    25441 ctggtgggcg agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggctggat
    25501 gacggcagca gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa
    25561 gcgcgggcaa agttgcgcgc gaagcacgta ggctttgttt ttcagtcatt tatgttaatt
    25621 cctaccctta acgcgctgga aaacgtcgag cttccggcgc tgctgcgcgg tgagagtagc
    25681 gcagaaagtc gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg
    25741 gatcatcttc cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc
    25801 tttaacggtc gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag
    25861 acgggcgata aaattgccga tctgcttttt tccctcaacc gtgagcatgg caccacgttg
    25921 attatggtga cccatgacct gcaactggcg gcacgctgtg accgctgctt acggctggtg
    25981 aacgggcagt tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgctcgc
    26041 cgtcgctatt aattgtctgg ctggcgttaa gcctggcggt ggcctgtgtg ctggcgctgg
    26101 gcaatattag cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg
    26161 gcgatcgggc gttgcgcagt tcgcgcgaag tgccgcaagc atggctggag gaagcgcaaa
    26221 agcgcggcct gaaagtcggc aagcagctga ctttcgccac catgaccttt gcaggcgaca
    26281 cgccgcagct ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc
    26341 aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga
    26401 tggcactgct taacctgaaa acgggcgata ccattgacgt gggcgatgcc accttgcgga
    26461 ttgccggaga agtgattcag gaaccggatt ccggttttaa tcctttccag atagccccac
    26521 gcctgatgat gaatctggcg gatgttgata aaaccggagc cgtgcagccg gggagtcggg
    26581 tcacctggcg ttataaattc ggcggcagcg agaaccagct cgacggctat gagaaatggt
    26641 tgttacccca gcttaaacct gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc
    26701 tggggcgatc gatagaacgt tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc
    26761 tgctggcggt ggcggcggtc gcggtggcga tgaatcatta ctgtcgcagt cgctacgact
    26821 tggtggcgat cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ttaattgtcg
    26881 gtcagtggtt gatggtgctg gtactttcag ccgttaccgg tggggcgata ggtctgttgt
    26941 tcgaaaacgt gttgatggtg ctgctcaaac ccgtgctacc tgccgcttta cctcctgcca
    27001 gcctctggcc gtggctgtgg gcgctcggca ccatgacagt catctcgctg ctggtgggcc
    27061 tgcgaccgta ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg
    27121 tcgcgaacgt ctggccgctc aagttttatc tgccgattgt cagtgtggtg gttgtgctgc
    27181 tgcttgccgg gttaatgggc ggcagtatgc tgctttgggc ggtgctggcg ggcgcggtag
    27241 tactggcttt tctgtgcggt gttctgggct ggatgctgct gaatgtactt cgccgcatga
    27301 cgctgaaatc gctacctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa
    27361 cgttaagcca gctttcggca ttttcgctct cctttatgct gctggcactg ttgctggtgt
    27421 tgcgtggcga tctgctcgac cgttggcaac aacaactgcc gccagaaagc ccgaactact
    27481 ttttaatcaa catcgctacg gaacaggtta cgccgctgaa agcgtttctc gcggaacatc
    27541 agatagtccc ggaatcgttt tatccggtgg tgcgggcgcg gttgacggcg attaacgaca
    27601 agccgacaga aggcaatgga gatgaggcgc tcaaccgcga gctgaatctc acctggcaaa
    27661 atacgcggcc tgatcataac ccgatcgtcg ccggtaactg gccgccaaaa gccgatgaag
    27721 tgtcgatgga agaggggctg gcgaaacgct taaacgttgc cctcggcgat accgtgactt
    27781 ttatgggcga tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg
    27841 aaagtctgcg gcctaatttc tattttattt tcccggaagg ggcattagac gggcaaccgc
    27901 agagctggct taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc
    27961 gccagttccc gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg
    28021 tgctggagca ggtaagtcgg gcgctggaag tgatggtggt gctggtcacc gcctgcggca
    28081 tgttgctgtt gctggcgcag gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg
    28141 tgtggcgcac acttggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg
    28201 ccatgctcgg gtttgtatcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcgg
    28261 tactgcaatc gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgtgc
    28321 tgccgtgcag cggtgcgcta ctgctgtcgt tttgcggcgg ctggctgggt gcgcgactgc
    28381 ttaagggtaa ggcgctgttc aggcagtttg cggggtgatg acaggtgtta gattgcctga
    28441 tgcgctacgc ttatcaggcc tacgaagatc gtgcaatata ttgagttttc gaaattttgt
    28501 aggccggata aggcgttcac gccgcatccg gcagttgtgc gccgatgcca gatgcgacgc
    28561 aggcgcgtct tatcatgcct acgaaacagc actcacctgt aggctggata aggcgttcac
    28621 gccgcatccg gcatgaacag cgtgcataga atcccccatc actgcgcctt aacccattcc
    28681 accacttccg cccactcacc gcgaaagaca actttttccg cttttttctc aagctgatag
    28741 cgatacatcg ggtcgtaata ttcttcaagt aacggcacca gccaggccag atgaccgtcg
    28801 gtgctgccgg tggtgagttg cgttgtcagc gccgcatcca gccttgcagc cagttcgtta
    28861 tagcgttgta gtcccagccg acgtttaatc gccgaaagcc cgtgatgcag gtattcgcaa
    28921 tactcctgcc agccctgttc gtcgccataa gcgtgggtaa aatcatgatg catacgcaag
    28981 aaatactctt cgttcaggcg ttcaagacgg atctcaaacg gatcttctac caccgtaatc
    29041 gtcgcctgag tcattcgttc gcgcagacat tccggcaggt gattcgaacc gatcatccgg
    29101 ctttcatctt ccagcaccca caggcgcaaa tcctgatggg cgtcggtttt tagcatttcg
    29161 gcagccagca tgttttcaaa actcgcctgg ctaagttgtg gttgtaacgt gcgaccaaaa
    29221 gcagaaccgc gatggcgcgc cagcccttcc agatcaacac cgttcggttg ttgttgcact
    29281 aacagcgttt tgccgcagcc ggtacaaccg ccaatcagca ctatcggttt ttgtgacagt
    29341 tcaatggtcg cctgaatcgc ggtctggcgc agtgccttat aaccgccttc caccagcgga
    29401 taatcaatcc ccgcgtcatg cagccagcgt tgcacaatat gcgagcgctg accgccacgg
    29461 gcgcagcaga gaataccgtg cggattttgc aggcacgctg cccgccaggc gtccatgcgc
    29521 tgctgacgaa tttcacccgc caccagttta tgccccagtg ccagcgccgc gtctgaccct
    29581 tgctgtttat agcagatgcc aacggcggcg cgttcatcgt tattcattaa cggtaggttg
    29641 atagcggcgg gcattgcgcc ttgctcaaac tcgatggggg cgcgaacatc aataatgggc
    29701 gtatcagcaa tcagcagggc acgatagtcc tgttccgtgt gtctctcttg catagtaaaa
    29761 agtgaacctc aaatcagctt gcgcgctatt ttacgcgcca acgcgcaagg aaacttgatt
    29821 tttaactgcg tgggttgccg aaaatttcta aaaatccgct gatttccggc ctgcgctggg
    29881 taaacaaggt cacaatatct tctaccgctt tgccgctgcc aaatttgcgc catgccagac
    29941 tcaatggcga aggagggcgc atcgttggga ttacccggct gaccagttgt tgattatcga
    30001 tcattgactg gcaaagtgat tttggcaaaa aaccaatgcc aacgcccgcc agatgggcgg
    30061 cgattttcgt ttccatatcg ggaacaataa tctctttttg ccccggcaat cgccaggcga
    30121 cgcgtttggt taaggtgcgg gcgctgtctt caatattgac cgccggaaag cgccgcaact
    30181 gtgcttctgt tagcggctct tcaacgttcg ccagcggatg atccgccgcc atgacaaagc
    30241 gccattgcac cgatcctaag ggatcaagac taaaggtatt tgccagcgcc tcggttcccg
    30301 tgacgccgat agccagtgaa aaaccttcgt acaatagcga gtcccagacg cccatataga
    30361 tttgtcggga gatgtgaaat tgggtaaacg ggtaacgttc atttagccac gccagcagcc
    30421 tggcaacggc ctgagggtta tagagcaggt tgttgatgac gatattcacc tggcgttcca
    30481 cgccatcatt cacctgttgt aactcgctgg gcatactttc cagccagctc agccagtctc
    30541 tggcctgaca aagtagatgc tcgccagccg ccgtcaacgt cacgctgcga gtcgtgcgga
    30601 aaaacagcgc cactccggta ttctcttcca gaagtttaat gcgataactg atcgtcgccg
    30661 tggttttaca taatcgttct gccgcttttg aaaaacttcc tgtttcagca accgcaatga
    30721 aagtccgcaa ggtttctgga tcgaacatct tcaggtatcc ccttttaaat ccgcaagttg
    30781 cgtgattttc ttatcctctg atttatcagt atttttacat gataaccctg ttcaatttgt
    30841 ggactaaatc tagttttgga aaaatattcc aacttttgtg ttgatgttgt tctcttaagg
    30901 ttttagattg cctgttattg aaaccaagct gaccggtcgg cggtggttga acggaattat
    30961 gttacaagga caaaaagatg aaacttcagg tattaccgtt aagtcaggaa gccttctgtg
    31021 cttatggcga cgtaatcgaa acgcagaaac gggatttttt ccatattaac aatggactgg
    31081 tggagcgtta ccacgatttg gcgctggttg agattcttga gcaagaccgt acgcttatca
    31141 gcattaaccg cgcgcaaccg gcgaatctgc cgctgaccat tcacgaactc gaacgtcatc
    31201 cgttgggcac tcaggccttt atcccgatga aaggtgaagt gtttgtggta gtcgtggcgt
    31261 taggtgacga caaaccagac ctgtcaaccc tgcgggcgtt tattaccaac ggcgaacagg
    31321 gagtgaatta ccatcgtaac gtctggcatc acccactttt cgcctggcag cgcgtcaccg
    31381 attttctgac catcgatcgc ggcggcagtg acaactgtga tgttgaaagt attcctgaac
    31441 aggaactctg ttttgcgtga cgcctgcaac cgacttgcat aagataaacg aattgttcat
    31501 tgtttatgct catttgtagg tcggagttaa cgtaggtatg acggaagtta gacggcgcgg
    31561 caggccagga caggcggagc ctgtggcaca gaagggtgca caggcgttag agcggggaat
    31621 tgcgattctg caatatctgg aaaaaagtgg gggaagttcg tcggttagcg atatctctct
    31681 caatctggat ttgccgctct ccacgacctt tcgcttgctg aaggttttac aggcagcgga
    31741 ttttgtctat caggacagtc agttaggctg gtggcatata ggattaggtg tctttaacgt
    31801 cggtgcagcg tacatccata accgcgatgt actctccgtc gccgggccgt ttatgcgccg
    31861 cctgatgtta ctttccggcg aaacggtcaa tgtcgcgatc cgtaacggca atgaagcggt
    31921 attaattggt cagttagagt gtaaatcgat ggtcaggatg tgtgcgccgc tgggcagtcg
    31981 tctgccgctg catgcttccg gagcgggcaa ggcgctgctt tatccgctgg cggaagaaga
    32041 gttgatgagc atcattctgc aaaccggttt gcagcagttt acgccaacga cgcttgtgga
    32101 tatgcccacc ttgctgaagg acctggaaca agcgcgtgaa ctgggctata ccgtagataa
    32161 agaagagcat gttgtcggcc tgaattgcat agcttcagca atttacgatg atgtaggtag
    32221 tgttgttgcc gctatctcca tctccgggcc ttcatcaaga ctgacagaag atcgttttgt
    32281 cagtcagggt gagctggtca gagacaccgc ccgcgatatc agcacggcgt tgggactgaa
    32341 agcacatcta tagtgtctgt cgtatcccgc tctgcggagc gggttttttt gacaaaattt
    32401 gaaagttgga aaatttttcc aattaataga ggtaggaata aaatggcaaa aatgagagcc
    32461 gttgacgcgg caatgtatgt gctggagaaa gaaggtatca ccaccgcctt cggcgttccg
    32521 ggagctgcaa tcaatccgtt ctactcagcg atgcgtaagc acggcggtat tcgtcacatt
    32581 ctggcgcgtc atgtggaagg tgcttcgcac atggcggaag gttatacccg cgcaacggca
    32641 gggaatatcg gcgtatgtct ggggacttcc ggtcctgcgg gcacggacat gatcaccgcg
    32701 ctctattccg cttctgctga ttccattcct attctgtgta ttaccggcca ggctccgcgc
    32761 gcccgtctgc ataaagaaga tttccaggcc gttgatattg aagcaattgc taaaccggtc
    32821 agcaaaatgg cggttacagt tcgtgaagcg gcgctggtgc ctcgtgtgct gcaacaggcg
    32881 tttcacctga tgcgttctgg tcgaccgggt ccggtactgg tggatttacc gttcgacgta
    32941 caggttgcgg aaatcgagtt tgatcctgat atgtacgaac cgctgccggt ctacaaacct
    33001 gctgccagcc gtatgcagat cgaaaaagct gtagaaatgt taatccaggc cgaacgtccg
    33061 gtgattgttg ccggaggcgg ggtaattaat gctgacgcag ctgtactgtt acaacagttt
    33121 gctgaactga ccagcattcc ggtgatccca acgctgatgg gctggggctg tatcccggac
    33181 gatcatgaac tgatggccgg gatggtgggc ctgcaaaccg cgcatcgtta cggtaatgca
    33241 acgttgctgg cgtccgacat ggtgtttggt atcggtaacc gttttgctaa tcgtcatacc
    33301 ggttcggtag agaaatacac cgaagggcgc aaaatcgttc atatcgatat tgagccgacg
    33361 caaattggcc gcgtactgtg tccggatctt ggcattgtct ctgatgctaa agcggcgctg
    33421 acgctgctgg ttgaagtggc gcaggagatg caaaaagccg gtcgtctgcc gtgtcgtaaa
    33481 gagtgggtcg ccgactgcca gcagcgcaaa cgcactttgc tgcgcaaaac ccacttcgac
    33541 aacgtgccgg tgaaaccgca gcgcgtgtat gaagagatga acaaagcctt tggccgcgat
    33601 gtttgttatg tcaccaccat tggcctgtca caaatcgctg cggcacaaat gctgcatgtc
    33661 tttaaagacc gccactggat caactgtggg caggcaggcc cgctgggctg gacgattccg
    33721 gcggcgctgg gggtttgtgc cgctgatcca gagcgcaatg tggtggcgat ttccggtgac
    33781 ttcgacttcc agttcctgat tgaagaatta gccgttggcg cacagttcaa cattccgtac
    33841 atccatgtac tggtcaataa cgcctatctg ggtctgattc gccagtcgca acgtgccttt
    33901 gatatggatt actgcgtaca gctcgcgttc gaaaatatta actccagcga agtgaacggt
    33961 tatggcgtcg accacgtaaa agtagcggaa ggtttaggtt gtaaagcaat tcgcgttttc
    34021 aaaccggaag atattgcacc agcctttgaa caggcaaaag tcttaatggc gcaatatcgg
    34081 gtaccggtag tcgtggaagt tattctcgag cgtgtgacca atatttcgat gggcagtgaa
    34141 ctggataacg tcatggaatt tgaagatatc accgataacg cagcggatgc accgactgaa
    34201 acctgtttca tgcactatga ataagggaga taaataatgt tacgtttctc tgctaattta
    34261 tcgatgttat ttggtgaata tgattttctc gcccgttttg agaaagctgc gcagtgtggt
    34321 tttcgcggcg ttgaatttat gttcccttat gactacgaca ttgaagaatt aaaacaggtg
    34381 ctggcgagta ataaactcga acatacgctg cacaatttac cggcaggtga ctgggcggcg
    34441 ggcgagcggg gtattgcctg tattcctggc cgtgaagaag agtttcggga tggcgtagca
    34501 gcagcgattc gttatgcttg tgcgctgggt aataaaaaaa ttaactgtct ggtcggtaaa
    34561 acaccgactg gtttcagcag tgaacagatt cacacaacgc ttgtagaaaa cctgcgttat
    34621 gccgcgaata tgctgatgaa agaagatatt ttattactga ttgaacctat taaccatttt
    34681 gatattcctg gtttccatct caccggaacc cggcaggcac tgaaactgat tgacgatgtt
    34741 ggttgctgca atttgaaaat tcagtatgac atttatcaca tgcagcggat ggaaggtgaa
    34801 ttaactaata ccatgactca gtgggccgat aaaattggtc acctgcaaat tgctgataat
    34861 ccgcatcgcg gcgaaccggg aaccggagaa attaattacg aatatctctt tacggtaatt
    34921 gaaaattctg actacaacgg ttgggttggc tgtgaatata aaccccaaac caccacggaa
    34981 gctggtttag gctggatgga tccgtaccgt taaaacgtaa cgctattcag acaatgcttt
    35041 tttaggccgc taagttggca ggggatcgtg ttgtctgaat tcaggaaaag cgaaatttaa
    35101 aagaggttaa ttatgaaact gggatttatt ggcttaggca ttatgggtac accgatggcc
    35161 attaatctgg cacgtgctgg tcatcaactg catgtcacaa ccattggtcc ggttgctgat
    35221 gaattactgt cactgggcgc cgttagtgtt gaaactgcgc gccaggtaac agatgcatcg
    35281 gacatcattt ttattatggt gccggatacg ccacaggttg aagacgttct gtttggtgaa
    35341 aatggttgta ccaaagcctc gctgaagggc aaaaccattg ttgatatgag ctccatttcc
    35401 ccgattgaaa ctaaacgttt cgctcgtcag gtgaatgaat tgggcggcga ttatctcgat
    35461 gcgccagtct ccggcggtga aatcggcgcg cgtgaaggga cgttatcgat tatggttggc
    35521 ggtgatgaag cagtatttga acgtgtaaaa ccgctgtttg aactgctcgg taaaaatatc
    35581 actctcgtgg gcggtaacgg cgacggtcaa acctgcaaag tggcaaatca gattatcgtg
    35641 gcgctcaata ttgaagcggt ttctgaagcc ttgttgtttg cttcaaaagc cggtgcggac
    35701 ccggtacgtg tgcgccaggc gctgatgggc ggctttgctt cctcacgtat tctggaagtt
    35761 cacggtgagc gcatgattaa acgcaccttt aatccaggtt tcaaaattgc gctgcaccag
    35821 aaagatctca acctggcact gcaaagtgcg aaagcactgg cgctgaacct gccaaacacc
    35881 gcgacctgcc aggaattgtt taatacctgt gcggcaaacg gtggcagcca gttggatcac
    35941 tctgcattag tgcaggcgct ggaattaatg gctaaccata aactggcctg atacccgcaa
    36001 taaaaatggc cgatatcaga aaatgaatcg gccagcaata ttaaaaaaag aaagcagcca
    36061 aagatgttgc ttcagtatta aaaataatat ttttatttta tttgttcctc atagctagat
    36121 taaaacaacg ttattcgata cgtgaaatta agagggattt atggaacatc agagaaaact
    36181 attccagcaa cgcggctata gcgaagatct attgccgaaa acgcaaagcc agcggacctg
    36241 gaaaacattt aactatttta ccttatggat gggttcggtt cataacgttc ccaattatgt
    36301 gatggtcggc ggctttttta ttctcggctt gtctaccttt agtattatgc tggcaattat
    36361 cctcagtgcc tttttcattg ccgcggtaat ggtattaaac ggtgctgcgg gcagtaaata
    36421 cggcgtaccg tttgccatga tcctgcgtgc ttcttacggc gtacgtggtg cactgtttcc
    36481 cggattatta agaggcggta ttgctgccat tatgtggttt ggcctgcaat gttacgcggg
    36541 gtcactggcc tgcttgattc tgattggcaa aatctggccg ggatttttga ctctcggtgg
    36601 cgatttcacg ctgttaggcc tttctctacc gggcttaatt acttttttac tcttctggct
    36661 ggttaacgtt ggtattggtt ttggcggcgg aaaagtttta aataaattca ctgccattct
    36721 taacccgtgc atctatatcg ttttcggcgg tatggcgatt tgggcgattt cgctggtcgg
    36781 gatcggtcca atctttgact acattccggg cggtattcag aaagcagaaa acagtggctt
    36841 cctgtttctg gtggtgataa acgcggtggt tgcggtctgg gcggcaccgg cggtgagcgc
    36901 atccgacttt acgcaaaacg cccactcgtt tcgtgagcag gcgctggggc aaacgctggg
    36961 tttagttgtg gcctatattc tgtttgcggt cgccggggta tgtattattg ccggagccag
    37021 tattcattac ggcgctgaca cctggaacgt gctggatatt gttcagcgtt gggacagcct
    37081 gttcgcctcg ttctttgcgg tactggttat tctgatgacg actatctcta ctaacgccac
    37141 cggtaatatt attccggctg gttatcagat tgccgccatt gcaccgacga aactgaccta
    37201 taaaaacggc gtactgattg ccagtattat cagcctgctg atctgcccgt ggaaattaat
    37261 ggaaaatcag gacagtattt atctgttcct cgatattatc ggcggaatgc ttggtccggt
    37321 aattggtgtg atgatggcgc attattttgt cgtgatgcgt gggcaaatta atcttgatga
    37381 actgtacacc gcacctggcg attataaata ttacgataac ggttttaacc tcaccgcgtt
    37441 ttcagtaact ctggtggccg ttattttatc tcttggcggt aagtttattc cctttatgga
    37501 accgttatca cgtgtttcat ggtttgtcgg cgtcatcgtc gcctttgcgg cctacgcctt
    37561 attaaagaaa cgtacaacag cagaaaaaac aggagaacaa aaaaccatag gttaattaat
    37621 cacgatattg aacattgagt taaaaaccaa tctgtatttt acaaggagtt tgttatgtct
    37681 tttgatttaa tcattaaaaa cggcaccgtt attttagaaa acgaagctcg cgttgtagat
    37741 atcgccgtta aagacggaaa aattgctgct atcggccagg atctgggcga tgcaaaagac
    37801 gttatggatg cgtctggtct ggtggtttcg ccgggcatgg ttgatgcgca cacccatatt
    37861 tctgaaccgg gtcgcagcca ctgggaaggt tatgaaaccg gtactcgcgc agcagcaaaa
    37921 ggtggtatca ccaccatgat cgaaatgccg ctcaaccagc tgcctgcaac ggttgaccgc
    37981 gcctcaattg aattgaagtt cgatgccgct aaaggaaagc tgactatcga tgcggcacaa
    38041 ctcggtggcc tggtgtctta caacattgac cgtctgcatg agttggatga agtgggtgtt
    38101 gtcggtttca aatgcttcgt tgcgacctgt ggcgatcgcg gtatcgacaa cgacttccgt
    38161 gacgtcaatg actggcaatt cttcaaaggc gcgcagaagc tgggtgaact aggccagccg
    38221 gtgctggtgc actgcgaaaa cgcgctgatc tgtgacgcac tgggcgaaga agcgaagcgt
    38281 gaaggtcgcg taactgccca tgactatgtg gcttcgcgtc cggtatttac cgaagtggaa
    38341 gcgattcgcc gcgtactgta cctggcgaaa gtcgctggtt gccgtctgca cgtttgccac
    38401 gtcagcagcc cggaaggtgt tgaggaagtg actcgtgcac gtcaggaagg tcaggatgtt
    38461 acttgtgaat cctgcccgca ttactttgtg ctggataccg atcagttcga agaaatcggt
    38521 actctggcga agtgttcacc gccgatccgc gatctggaaa accagaaagg catgtgggaa
    38581 aaactgttta acggtgaaat agactgcctg gtttccgacc actctccatg cccgccggaa
    38641 atgaaagccg gtaacataat gaaagcgtgg ggcggtatcg ctggtctgca aagctgcatg
    38701 gacgtgatgt tcgatgaagc ggtacagaaa cgcggaatgt ctctgccaat gttcggcaaa
    38761 ttaatggcga ctaacgcagc agatattttc ggtctgcagc aaaaaggccg tatcgcccca
    38821 ggaaaagatg ccgacttcgt cttcattcag ccgaatagca gctatgttct taccaatgac
    38881 gatctggaat atcgccacaa agtcagcccg tatgttggcc gtactattgg cgcgcgtatc
    38941 acgaaaacca tcttacgtgg tgatgtgatt tacgatatcg aacagggctt ccctgttgcg
    39001 ccgaaaggtc aatttatcct taaacatcag cagtaatctg gccctgcaat gcccgtcctt
    39061 gtggcgggca ttctccggtt aaggtgtgtt tatgttcaat tttgcagtgg gccgcgaaag
    39121 cctgttatca ggatttcagt ggtttttctt tattttttgc aacacggttg tggttcctcc
    39181 tacgctactt tctgcttttc agttgccgca aagtagcctg cttacgctca cgcaatatgc
    39241 ttttcttgct accgcactgg cctgcttcgc tcaggcgttc tgcggtcatc gtcgcgctat
    39301 tatggagggg ccgggcggcc tgtggtgggg aaccatcctg actatcaccc ttggcgaagc
    39361 atcgcgcggg acaccgatca acgatatcgc taccagtctg gcggtgggga ttgcgctctc
    39421 cggcgtgctg acgatgttga ttggttttag cggattaggc catcgcctgg cacggttatt
    39481 tacgccgtct gtgatggtct tgtttatgtt gatgctgggt gcgcaactga ccactatctt
    39541 tttcaaaggt atgctcgggc tgccgtttgg catagccgac ccgaatttta aaattcaatt
    39601 accgccgttc gcgctctcgg tggcggtgat gtgcctggta ctggcgatga ttattttcct
    39661 gccacaacgt tttgcccgtt acggcctgct ggtcggtaca ataaccggct ggttgttgtg
    39721 gtacttttgc tttccttctt cgcattcgct ctccggtgag ttgcactggc agtggttccc
    39781 gctcggcagt ggcggtgctt tgtcgccggg gattattctg acggcggtga ttacaggtct
    39841 ggtaaatatc agcaatacct acggtgcgat tcggggcacg gatgttattt atccgcagca
    39901 gggcgcgggg aatacgcgtt atcgtcgcag ctttgtggcg accggattta tgacactgat
    39961 aaccgtgccg ctggcggtaa ttccattttc accgtttgtt tcatccattg gtttattaac
    40021 ccagactggc gattacacgc ggcgttcgtt tatttatggc agcgttattt gcctgctggt
    40081 ggcgctggtt ccggcactca cgcgactgtt ttgcagtatc cctttacccg tgagtagtgc
    40141 ggtcatgctg gtttcttatc tgcctttact cttttccgcg ctggtgttta gccagcaaat
    40201 aacgtttacc gctcgcaata tttatcgact cgcattgccg ttatttgtcg gcatattttt
    40261 aatggcatta ccgcctgtgt atctgcaaga ccttccatta acgcttcgtc ctctgctcag
    40321 taacggctta ctggtcggaa ttttactggc tgttcttatg gataacctta ttccgtggga
    40381 acgcatcgaa taatttgttg aaaaaggatt gataatgaag attgtcattg cgccagactc
    40441 ttttaaagag agcttaagtg cagaaaaatg ttgtcaggca attaaagccg ggttttcgac
    40501 catatttccc gatgcgcact atatctgttt gccgatagcg gatggcggcg aagggacggt
    40561 ggaggcgatg gtcgccgcga cggacggtaa catcgtgaag cttgaagtct gcgggccgat
    40621 gggcgaaaaa gtgaaagctt tttatggcat taccggcgac gggaaaacgg cagtgattga
    40681 gatggcggca gcaagtggcc tgatgctggt cgcgcctgaa aagcgtaatc cgttgctggc
    40741 ctccagtttt ggtacggggg agttaattcg ccatgcgctg gataacggca ttcgccatat
    40801 tattctcggc attggcggca gtgcgacggt cgacggcggt atgggcatgg cgcaggcgct
    40861 cggtgtgcgt ttccttgatg ccgacggtca ggcgctggcg gctaacggcg gcaatttagc
    40921 gcgcgtggca agcattgaga tgaatgaatg cgatccgcgt ctggcgaact gtcatattga
    40981 agtagcatgt gacgttgata acccgctggt aggggcacgc ggcgcggcgg cggtgtttgg
    41041 cccgcaaaaa ggggcaacgc cggagatggt cgaagagctg gaacaggggc tgcaaaatta
    41101 cgcccgtgtt ttacaacagc taactgaaat caacgtctgc cagatggcgg gtggtggcgc
    41161 ggcgggcggt atgggcattg cggcggcggt attcctcaat gcggatatta aaccgggtat
    41221 tgagattgtg ttgaatgcgg tcaatcttgc gcaggcagtg cagggtgcag cactggtgat
    41281 caccggggaa gggcgcatcg actcgcaaac ggcaggcggt aaagcgccgc tgggtgtggc
    41341 atcggtggcg aagcagttta atgtcccggt gattgggatt gctggcgtat tgggtgatgg
    41401 cgtggaagtg gtgcaccagt acggcattga tgcggtattc agcattttgc cacgtctggc
    41461 acctttagcc gaagtgctcg ccagcggtga aaccaatctc ttcaacagtg cgcgaaatat
    41521 tgcctgcgcc attaaaatag gtcagggaat taaaaactga ctattacctt taaagcggat
    41581 gcgatttata tcgcatcttt ttttacctga attgactaca aaaaaagcca cggtataaac
    41641 cgtggcaaaa tccaacatag ctaaaaataa tcaggcgagt ggtatgactt aaatctctac
    41701 gtcgcggttg caatctttcg agtaaatata gctgaacgct tcaccacgcc ctacgccata
    41761 accagcctgt aaagaataag cgcccataaa gatgtaatcg ccttttttca ccgggatcca
    41821 gttattgtcg aggttataaa ccccctgacc ggaaagaata taggcaccgt gttcctgaac
    41881 gtgggtttcg atataaccgt ggctggcacc tggtgcaaaa gagaggatat gcatgttcat
    41941 atcaaaacct aactctttgg gcagaaaatc cagcagaata acatcgtcca tgccttcata
    42001 atgaatgcgt tccagttcgc tggcattgcc agaaaccagc cacggcgcat gaccttctac
    42061 cggaatatag cgacgcttat ataaaaagat ttggctgtct tcggcctggg cgttaacaaa
    42121 cgtcattaag gagcctggcg ggcaataaag atagccacct tcacttaagg taaatgtttt
    42181 cccttcggct ttggtagtga tatttccaga gatcacatac aggaacgttt caatgccttc
    42241 gccaccgaag ccctgttggt tgccgccgtt ttgatgcagt gtgaccagat aatcaacaaa
    42301 agaggcaccc agctttggcg tggagaggat tgtcgcgtca caattttcaa agcccggaat
    42361 aatatttttt accagaccgt ctggtgttaa cagtgcgaaa ttaccgtgtt taacaatcgc
    42421 acggttagcc agtaaatctt cgcggtaacc ggtgacgtta tttaaatatc ccatttataa
    42481 ctccttattt ctgccaggca agttgataaa gcatgagtgc caacgttttg accccttcgg
    42541 caaggtcggt aatattggtg cgttccgccg ggttatggct gatcccgttg atgctgggaa
    42601 tgaaaatcat gcaggtcggc acgcgaggcg cgaaaatttg cgcgtcgtgc ccggcaccac
    42661 tgtgcatcac ccggtaattc agtttttcgc tttcacacaa ttctgtcagg gtggcgacca
    42721 gctccttatt catcggcacg ggttcttcgt ccatccataa atcgatatca ataccaatat
    42781 ccatttcatc gcaaatcgcc cgcatgtcgt tttctaactg ttgggtgaaa tcgcgcagca
    42841 cggtagcgtc ggtatgacga caatcaatgg tgaacgtggt tttacccggc actacattca
    42901 ccgtattcgg gcgcggctct actttgccaa aggtcagaac cagcgggtcg cccatctttt
    42961 tcgctttttc gacagactga tggcaaatgc gactgaaagc gtaaaccgta tcacgacgat
    43021 aacccatcgg cgtggtgcct gcatggtttg attcgccatt cagcgtcacc gtataacgac
    43081 gctgcccgac aattgcattc accacgccaa ttgattgccc attactttcc agcacacagc
    43141 cctgttcgat atgcagttcg acaaaggctt taatatcctg acgcggagtt agtggggcgt
    43201 tcggcagagt aaatccgcaa gccttcatcg catcgacaaa actatttcct ttggcatcac
    43261 agatattccg cacgtcgtca ggattcgcca gcccaaaaat atttttactg ccccagaaaa
    43321 catacgggaa gcggctgcct tcttcttctg ccatcgccac cacttcgacc gtacgtaacg
    43381 gcgcgccgta ttgcgttttc agccagtcaa tagccagcca cgccgccagc gcaccgaatt
    43441 gcccgtcgag gttaccgccg ttaaccacgg tatcgatatg cgaaccgctc agaaccactt
    43501 cctgtggata ttcggtgcca ctcaggcgac cgtataaatt ccccacttca tcgaaacgtg
    43561 tttccagccc gcttgctgcc attctttttt taaactgctg ctgggtttcc agccattccg
    43621 gcgaataaag taaacgggtc atcccacccg ctgggtcagc gccaaaagag gaaagccagg
    43681 gcagcgtttc ttctatagct tgacggaaat gtgtaatcat aagaaagtcc tgtctcaata
    43741 attattgcgc aaagggattt ttcgtttcgt atgacgtgtt ataaagcgcg tcggaaatta
    43801 aatactggta aatatcatca acaatttcga tgccttcgac ggcggctttg cgttgtttaa
    43861 tatcctgatc ctgtccggga taataaacct gattaaaacc gggcgcgggg gtaatggtat
    43921 ttaattcgcg catggtctgg ctaagatgtt gacggaataa ttcgctggag gagaaaaagt
    43981 tcgggttaat aactatatgt aattgcccca aattacgccc ggcgtgtaaa tcgtcataca
    44041 tcgaactaac ctgtcgcccg aacggtaagc cgagtaagac gcctgagagg acgtcaatca
    44101 tcatcatcag gccataccct ttcggcccag cggcggggag cagagcatgt accgcgaacg
    44161 gatcggttgt tggtgcaccg tttttatcga ctgcccaggt atccgggata gacatattgc
    44221 gcgagcgggc gtcgagcact tttccccatg cctgtacggt agtcgccata tcaaaggtaa
    44281 ggatctcgtc gccttctccc ggcgcggcaa aggccagtgg gttagtgccg tagtaaattt
    44341 ccgcgccgcc aaacggcacc accattggat cggactggca cagcgaaatg ccaatcaatc
    44401 cggcgcgggc agcctgctgc acaaaataag agattgcgcc gctgtgaccc atccggctga
    44461 taccgaccac cgcaacgcct ttttgctggg cggttttgat ggcatgttcc atacccattt
    44521 tcgccgcgac ctgtccggcg gcattgtcgg catgtaaaat tgccgagcac ggtccggttt
    44581 cctcaagacg aaactccggt tcgcggttgg tgccgccttt tgaaatgcgt tcggcgtagt
    44641 attcaacgcg caccgcgcca tgagagtgga tccctctggc atcggcgtaa accaatactt
    44701 cagccacggt tgcagcgtgc tcacgtttta acccagcctg gcagagttta ttctcgatta
    44761 gctggtggag tgtttcccga ctgattttca tctgtcttcc tttttaacga cggtgtgaag
    44821 catgactgca attaacatac agggaaaata tctgg
//
LOCUS       DAZKHK010000022        44618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKHK010000022 DAZKHK010000000
VERSION     DAZKHK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.022"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            169..3927
                     /gene="yfaL"
                     /locus_tag="ACHOS5_001272"
                     /pseudo
     CDS             169..3927
                     /gene="yfaL"
                     /locus_tag="ACHOS5_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
     gene            complement(4067..4789)
                     /gene="ubiG"
                     /locus_tag="ACHOS5_001273"
     CDS             complement(4067..4789)
                     /gene="ubiG"
                     /locus_tag="ACHOS5_001273"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP9776115.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4936..7563
                     /gene="gyrA"
                     /locus_tag="ACHOS5_001274"
     CDS             4936..7563
                     /gene="gyrA"
                     /locus_tag="ACHOS5_001274"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP9776116.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7712..9400
                     /gene="yfaA"
                     /locus_tag="ACHOS5_001275"
     CDS             7712..9400
                     /gene="yfaA"
                     /locus_tag="ACHOS5_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP9776117.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYSGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPNDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9397..10020
                     /gene="yfaT"
                     /locus_tag="ACHOS5_001276"
     CDS             9397..10020
                     /gene="yfaT"
                     /locus_tag="ACHOS5_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP9776118.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10044..14558
                     /locus_tag="ACHOS5_001277"
     CDS             10044..14558
                     /locus_tag="ACHOS5_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP9776119.1"
                     /translation="MGTGLTNADDSLPSSNYVPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYSGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDGSST
                     LQLQHISPERSYILGKDAEGGVFVSENFFFESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDNGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELQSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKSLYMKWSMPTVYRMGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTKDFSEADTSDINDSMILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14559..16208
                     /gene="yfaQ"
                     /locus_tag="ACHOS5_001278"
     CDS             14559..16208
                     /gene="yfaQ"
                     /locus_tag="ACHOS5_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP9776120.1"
                     /translation="MNWYRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADMWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAVKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTYLQQNADRDGDCLSIPDSSATQRVSASPATVGARTMTAWT
                     QDLIYAGDPVHYHGSRVTEGTLSWRHATAQAGQGERYDQILAFAYPDNNLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRVLQDEMGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16213..16989
                     /gene="yfaP"
                     /locus_tag="ACHOS5_001279"
     CDS             16213..16989
                     /gene="yfaP"
                     /locus_tag="ACHOS5_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP9776121.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17063..18247)
                     /gene="atoB"
                     /locus_tag="ACHOS5_001280"
     CDS             complement(17063..18247)
                     /gene="atoB"
                     /locus_tag="ACHOS5_001280"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFP9776122.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAEKVCGFTVNKVCGSGLKSVALATQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQVADIDLIEANEAFAAQFLAVGKTLGFE
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18278..19600)
                     /gene="atoE"
                     /locus_tag="ACHOS5_001281"
     CDS             complement(18278..19600)
                     /gene="atoE"
                     /locus_tag="ACHOS5_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFP9776123.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19597..20247)
                     /gene="atoA"
                     /locus_tag="ACHOS5_001282"
     CDS             complement(19597..20247)
                     /gene="atoA"
                     /locus_tag="ACHOS5_001282"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFP9776124.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20247..20909)
                     /gene="atoD"
                     /locus_tag="ACHOS5_001283"
     CDS             complement(20247..20909)
                     /gene="atoD"
                     /locus_tag="ACHOS5_001283"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFP9776125.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21105..22490)
                     /gene="atoC"
                     /locus_tag="ACHOS5_001284"
     CDS             complement(21105..22490)
                     /gene="atoC"
                     /locus_tag="ACHOS5_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFP9776126.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22487..24313)
                     /gene="atoS"
                     /locus_tag="ACHOS5_001285"
     CDS             complement(22487..24313)
                     /gene="atoS"
                     /locus_tag="ACHOS5_001285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFP9776127.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24480..27329
                     /gene="rcsC"
                     /locus_tag="ACHOS5_001286"
     CDS             24480..27329
                     /gene="rcsC"
                     /locus_tag="ACHOS5_001286"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP9776128.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27529..28179)
                     /gene="rcsB"
                     /locus_tag="ACHOS5_001287"
     CDS             complement(27529..28179)
                     /gene="rcsB"
                     /locus_tag="ACHOS5_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP9776129.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28224..29513)
                     /locus_tag="ACHOS5_001288"
     CDS             complement(28224..29513)
                     /locus_tag="ACHOS5_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9776130.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRIGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(29547..29801)
                     /gene="xisR"
                     /locus_tag="ACHOS5_001289"
     CDS             complement(29547..29801)
                     /gene="xisR"
                     /locus_tag="ACHOS5_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9776131.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(29809..29955)
                     /locus_tag="ACHOS5_001290"
     CDS             complement(29809..29955)
                     /locus_tag="ACHOS5_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776132.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(29976..30218)
                     /locus_tag="ACHOS5_001291"
     CDS             complement(29976..30218)
                     /locus_tag="ACHOS5_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776133.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            complement(30211..31113)
                     /locus_tag="ACHOS5_001292"
     CDS             complement(30211..31113)
                     /locus_tag="ACHOS5_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776134.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(31110..31334)
                     /locus_tag="ACHOS5_001293"
     CDS             complement(31110..31334)
                     /locus_tag="ACHOS5_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776135.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(31351..31713)
                     /locus_tag="ACHOS5_001294"
     CDS             complement(31351..31713)
                     /locus_tag="ACHOS5_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP9776136.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYENLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(31706..32572)
                     /locus_tag="ACHOS5_001295"
     CDS             complement(31706..32572)
                     /locus_tag="ACHOS5_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP9776137.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(32596..33180)
                     /locus_tag="ACHOS5_001296"
     CDS             complement(32596..33180)
                     /locus_tag="ACHOS5_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776138.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(33167..33727)
                     /locus_tag="ACHOS5_001297"
     CDS             complement(33167..33727)
                     /locus_tag="ACHOS5_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776139.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(34135..34497)
                     /locus_tag="ACHOS5_001298"
     CDS             complement(34135..34497)
                     /locus_tag="ACHOS5_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776140.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(34672..34872)
                     /locus_tag="ACHOS5_001299"
     CDS             complement(34672..34872)
                     /locus_tag="ACHOS5_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776141.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(34869..35060)
                     /locus_tag="ACHOS5_001300"
     CDS             complement(34869..35060)
                     /locus_tag="ACHOS5_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776142.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(35062..35439)
                     /locus_tag="ACHOS5_001301"
     CDS             complement(35062..35439)
                     /locus_tag="ACHOS5_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9776143.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(35525..36184)
                     /locus_tag="ACHOS5_001302"
     CDS             complement(35525..36184)
                     /locus_tag="ACHOS5_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9776144.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            36524..36817
                     /locus_tag="ACHOS5_001303"
     CDS             36524..36817
                     /locus_tag="ACHOS5_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776145.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            37060..37428
                     /locus_tag="ACHOS5_001304"
     CDS             37060..37428
                     /locus_tag="ACHOS5_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776146.1"
                     /translation="MRAELKQVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            37462..39120
                     /locus_tag="ACHOS5_001305"
     CDS             37462..39120
                     /locus_tag="ACHOS5_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9776147.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            39122..40090
                     /locus_tag="ACHOS5_001306"
     CDS             39122..40090
                     /locus_tag="ACHOS5_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP9776148.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRIQRRFSALTSLQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            40090..40953
                     /locus_tag="ACHOS5_001307"
     CDS             40090..40953
                     /locus_tag="ACHOS5_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9776149.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGID
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            41032..41457
                     /locus_tag="ACHOS5_001308"
     CDS             41032..41457
                     /locus_tag="ACHOS5_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9776150.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            41657..41812
                     /locus_tag="ACHOS5_001309"
     CDS             41657..41812
                     /locus_tag="ACHOS5_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9776151.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCDVRIRTGQTEVAVF
                     VDYESEK"
     gene            41938..44064
                     /locus_tag="ACHOS5_001310"
     CDS             41938..44064
                     /locus_tag="ACHOS5_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9776152.1"
                     /translation="MCASAQKTLIFIKGRFYAARPATSDRKSRKSVKSVEPDIADRAN
                     NWLKSYGLDYKLEQAPLNNEIDNALDAYFSKNGGTGGNRPDVKLLVQDKYGKQYPVLI
                     EYKGYKDRLIRLGSNGIIENKDARKEWNFKNINGYAVNGAVHYANALLQFTNYPDIIA
                     IGMTGWRDDGTGELHHEIGVWYVSKNNLGAGQKVGEFTDLSFLADKNVDGFLNKIKLL
                     NLPPEELEKIKASKEEEIDTRLSRLNNDIYQNEKGLGESDRVYLVVATVIATLGIPGK
                     LAPLDKKELTSSTEEDLRDGDIIFRKIRNFLRLKAVPETKREMILRSLQNTLWTENIN
                     KPVNGESQLKRVFVKVVDDLGEYYKIGLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTP
                     PYVATLLARLARVNKDSYVWDFATGSAGLLVAAMNEMLIDARENIHSPNELQLKEAQI
                     KAEQLLGLEVLSSIYMLAILNMILMGDGSSNILNKDSLADFDGKYGFGKTGEKFPADA
                     FILNPPYSAKGNGMIFVQKALSMMDKGYAAVIIQSSAGTGKATEYNKKILKENTLLAS
                     IKMPADLFIGKSSVQTYIYVFQVKIPHNAKQAVKFIDFSNDGYARSNRKKARNNLVDA
                     DRAKERYQEVVDLVHFGKGCLNIFTEDEYFEGTIDPDSGEDWNQTRPVDARPTLDDFK
                     KTVGDYLAWEVSQLLKKQGENNFAGK"
     gene            44106..44618
                     /locus_tag="ACHOS5_001311"
                     /pseudo
     CDS             44106..44618
                     /locus_tag="ACHOS5_001311"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT        11136 a        10523 c        11625 g        11334 t
ORIGIN      
        1 ccgatttacc ttaaagttat taatttgaat tgtgatgcat ctaaattaat tatcagataa
       61 taacaaaata gttattacat caggggcaga atttacaaaa gaaaaatacc ctatatcgca
      121 taatgcgatt aaatacgcca tcttatagaa aatgatggtt agatgaatat gcggattatc
      181 tctctacgca aggagtattt atctttactc ccgtcaatga ttgcatctct tttctctgct
      241 aacggtgccg cagcggtcat tgattcatgc cagggatatg atatcaaagc gaactgtcag
      301 gccagcaggc aaagcctttc aggcaatatg caggactgga gcatcgcaga tgggcaatgg
      361 ttgatttttt cgggtatggc caataatgcc agcggtggcg cagtattttt gcagcacagt
      421 gctgaattta cgatatcacc acaaaatgaa acagggatga ccctgtttgc tgataactcg
      481 gttagtggcg aatataataa tggtggggct atctttgcta aagaaaactc aacgataaat
      541 cttgcgaatg ttattttcga cagtaatgtt gcaggaggct atggcggagc aatctattct
      601 gccggtacca atgataccgg tgccgccgat ttacgtgtca ctaacgccgt atttcacaat
      661 aacatcgcta atgacggcaa aggcggtgca atttatacca tcaataatga cgtctattta
      721 agtgatgatg cttttaataa taaccaggca tatacatcaa caagttatag tgatggggat
      781 ggcggcgcca ttgatgttac ggataatagt acggataaca cacatctttc aggaaaaacg
      841 ataattaata atacctcctt taccaataac tatgcagaag gttatggcgg agcaatttat
      901 accagcagca ccacatcccc gtatcttatt gatatttctg ttgatgataa ttatgaccaa
      961 aacaatggtg tgatgattga tgaaaataac agcgcctcgg gatatgacca ttcggcgacg
     1021 gcagcggcgg ggggctttat gtatatcggt catagtgtgg ctgaatttaa cattgccgcc
     1081 gataaaacgc tggtaatagg caacaccagc aacgatggtg cgattgattc tcttgcctga
     1141 acgggagtca tcgtgaaaga gggagcggga gagttagtcc tcaatgccga caataatgcg
     1201 tttaccggcg agatgagtat ccagaatggc gaggtgacgc tggggcgcag cgatgagtta
     1261 atgaatgtcg gcgacacgca ctgtcagagc gatccgcagg attgctttgg tctgatggtc
     1321 ggcagcactg ttcattctga gtatcaggca gaactgaatg ttggcaatac acaacaaacg
     1381 tttgtgcact cattaaccgg ttttgctaat ggcattttga atatcgacgc aggcggcaat
     1441 gttaccgtaa accagggcgg tttttccggc tcgattcagg gtgaaggaca gttgacggta
     1501 gcgcaagatg gcagctatct cctgacaggg gcgcagtcga tggcattaac cggcgatatt
     1561 gtggtggaag ataacgccgt attgtcgctg gcggggaatc aggccgattt acgggcaatg
     1621 cagagcgatc cgcaatcgat cgtgctaaat ggcggagtat tggatctctc tgatttcacc
     1681 acctgggatg gcgatagctc atacaatgac ggtctgcaaa tcaacggcag tggggggaca
     1741 gtgattggca gtaatgatgt cgttgatatc agcagtggcg atgatttaca tattggcggc
     1801 agcgatgcca gccagaatgg cgtttatgtc gtcattaatg cgggggatca gcgcgtaacg
     1861 ctggcgaata acaacggtta tctcggtaat acgcaaatcg cctccggtac gcttgaagtc
     1921 agcgataatt cgcaattggg ggacacaagt tacaaccgtt cagtgatctt cacagatccc
     1981 caacaacaca gtgaaatgga tgtgacgact gatgttgaca ctcgctcggc tacaacaggt
     2041 cagggcagga atattgaaat gcgcgctgat ggcgaaatac acgttgagga tggcgtagat
     2101 acgcaatggg gcgggttgat ggcggatagt accgggcaac aactggatag cgtaagcacg
     2161 ttgactaaaa gtggcggcgg tacgctggag ttgaccgcca gcggcactgc gacgtctgcg
     2221 gtacgtgtgg aggacggaac gctaaaaggt gaagcggaga atattattcc ttatgtttct
     2281 tcactgtggg tgggggaaga cggtgttttt gaaaccgggc aaaatcagga tattcgctca
     2341 atcgatgcca cctctggcgg cgatatcgac ataaccgatg gtactgtgtt gcgattgacg
     2401 cagcaggata cgaaccaggt gctggatgcc tcgctgttta gcggcgacgg tacgctggtg
     2461 aatgccaccg atggcgtgac gctggcaggt gagcttaata ccaaccttga aactgacagc
     2521 ctgacttatc tttccgacgt gacggttaat ggcaatctga ccaatacgtc cggtgcgatt
     2581 agcctgcaaa atggcgtcgc tggcgacacg ctgacggtaa atggtgatta taccggcggc
     2641 ggtacgctac tgctcgacag cgaattaaac ggcgatgact cggcaagtga ccaactggta
     2701 ttgaacggta atactgctgg caacacggcc gtggtaatta accccattac gggtattggt
     2761 gagccgacat ctacaggtat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     2821 aacaatgcgc agttccgtct ggcaggaagc ggctacgtca atatgggggc gtatgactac
     2881 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtcac tccgacattg
     2941 ccgcccgatc cggacccgac tccagatcct gatcccacgc cggatcccga cccaaccccc
     3001 gaccctggac ctacgcctgc ttaccagccg gtgttgaatg ccaaagttgg tggatatttt
     3061 aataacctac gtgcggcaaa tcaggcattt gtgatggagc gacgcgatca cgctggtggc
     3121 gatggtcaga cgctgaattt acgcgttatc ggcgggcgtt atcattacac ggcggtaggg
     3181 cagttggctc agcatgaaga cacttctacg gtgcagctta gcggcaacct gtttagcggg
     3241 cactggggtg atgatggcga gtggatgcta ggggccgtgg gcggctacag cgataaccag
     3301 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     3361 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     3421 agctggctgc aatatgcgtg gtttaacaat gatgtttccg aacaagacga tggcgcggat
     3481 cattaccact cgtcggggat tatcgcctcg ctggaagccg ggtatcaatg gttaccgggg
     3541 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     3601 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gtgatgatat tcagacgcgg
     3661 ctgggtttac acagcgaatg gcgtactgct gttggtgtca cgccaacgtt agatctgaat
     3721 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     3781 gcggcgaagc aacggggtga aataaaagtg ggaataacgg gcaatatcag tcagcgagtt
     3841 tcgctgcgtg gtagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     3901 tttttgtcga tgacagtgaa atggtaatcg ctgtctgacc ggcactgttg ccggatgcgg
     3961 cgtaaacgcc ttatctggcc tacggggagc atttgtaggc ctgataagac gcggcaagcg
     4021 ccgcatcagg cttggctcgg ttgccggatg aatccaaaca acaacctcac ttattctgcg
     4081 tgtgcagcat atagttcaca tccacgccgg ggccgagctt aaaggaatta gtgatagggt
     4141 tgtaatgcag cccggtcata tgccgttctt taagactggt ctgatccacc cagcccagca
     4201 attctgccgg tttaataaac ttcttcacat catgtgtacc tttaggcacc atgcgcaaaa
     4261 tatattccgc accaaccacc gccatcagcc atgacttgcc gttgcggtta agcgtcgaga
     4321 aaaagacatc gccgcccggt ttcaccagtt gcgcacaggc tctgaccact gactgaggat
     4381 cggggacgtg ctccagcatc tccatacagg tcaccacgtc atactgtccg gcatgttttg
     4441 ccgcgtgctc ttccacggtt tcctgcacgt aatccacctg aatgccgctt tccagtgcgt
     4501 gcagttttgc cacctgcaat ggctcaaagc ccatatccag accggtcacc gtcgcgccct
     4561 cccgcgccat actctcggcc agaatgccgc cgccacaacc gacatcgagc acctttttgc
     4621 caaataaacc gccagcacgc tcggcaatat agcccagacg cagcgggtta atgcggtgca
     4681 gcggtttgaa ctcaccttca agatcccacc agcgggaggc gacggcttca aatttagcga
     4741 tctcttcgtg gtctacgttg tggtttaccg gcgatttttc ggcattcatt ggcacttcta
     4801 ctccgtaatt ggcaagacaa acgagtatat caggcattgg atgtgaataa agcgtatagg
     4861 tttacctcaa actgcgcggc tgtgttataa tttacgacct ttgaatccgg gatacagtag
     4921 agggatagcg gttagatgag cgaccttgcg agagaaatta caccggtcaa cattgaggaa
     4981 gagctgaaga gctcctatct ggattatgcg atgtcggtca ttgttggccg tgcgctgcca
     5041 gatgtccgag atggcctgaa gccggtacac cgtcgcgtac tttacgccat gaacgtacta
     5101 ggcaatgact ggaacaaagc ctataaaaaa tctgcccgtg tcgttggtga cgtaatcggt
     5161 aaataccatc cccatggtga ctcggcggtt tatgacacga tcgtccgtat ggcgcagcca
     5221 ttctcgctgc gttacatgct ggtagacggt cagggtaact tcggttccat cgacggcgac
     5281 tctgcggcgg caatgcgtta tacggaaatc cgtctggcga aaattgccca tgaactgatg
     5341 gccgatctcg aaaaagagac ggtcgatttc gttgataact atgacggtac ggaaaaaatt
     5401 ccggacgtca tgccaaccaa aattcctaac ctgctggtga acggttcttc cggtatcgcc
     5461 gtaggtatgg caaccaacat cccgccgcac aacctgacgg aagtcatcaa cggttgtctg
     5521 gcgtatatcg atgatgaaga catcagcatt gaagggctga tggaacacat cccggggccg
     5581 gacttcccga cggcggcaat cattaacggt cgtcgcggta ttgaagaagc ttaccgtacc
     5641 ggtcgcggca aggtatatat ccgcgcccgt gccgaagtag aagttgacgc caaaaccgga
     5701 cgtgaaacca ttatcgtcca cgaaattccg tatcaggtaa acaaagcgcg cctgatcgag
     5761 aagattgcgg aactggtaaa agaaaaacgc gtggaaggca tcagcgcgct gcgtgacgag
     5821 tctgacaaag acggtatgcg catcgtgatt gaagtgaaac gcgatgcggt cggtgaagtt
     5881 gtgctcaaca acctctactc ccagacccag ttgcaggttt ctttcggtat caacatggtg
     5941 gcattgcacc atggtcagcc gaagatcatg aacctgaaag acatcatcgc ggcgtttgtt
     6001 cgtcaccgcc gtgaagtagt gacccgtcgt actattttcg aactgcgtaa agctcgcgat
     6061 cgtgctcata tccttgaagc attagccgtg gcgctggcga acatcgaccc gatcatcgaa
     6121 ctgatccgtc atgcgccgac gcctgcagaa gcgaaaaccg cgctggttgc taatccgtgg
     6181 cagctgggca acgttgccgc gatgctggaa cgtgcgggcg acgatgctgc gcgtccggaa
     6241 tggctggagc cagagttcgg cgtgcgtgat ggtctgtact acctgaccga acagcaagct
     6301 caggcgattc tggatctgcg tctgcagaaa ctgaccggcc ttgagcacga aaaactgctc
     6361 gacgaataca aagagctgct ggatcagatt gcggaactgt tgcgtattct gggtagcgcc
     6421 gatcgtctga tggaagtgat ccgcgaagag ctggaactgg ttcgtgaaca gttcggtgac
     6481 aaacgtcgta ccgaaatcac cgctaacagc gccgacatca acctggaaga tctgatcacc
     6541 caggaagatg tggtggtcac gctttctcac cagggttacg tgaagtatca gccgctttct
     6601 gaatacgaag cgcagcgtcg tggcgggaaa ggtaaatctg ccgcacgtat taaagaagaa
     6661 gactttatcg accgactgct ggtggcgaac actcacgacc atattttgtg cttctccagc
     6721 cgtggtcgcg tctattcgat gaaggtttac cagttgccag aagctactcg tggcgcacgt
     6781 ggtcgtccga tcgtcaatct gctgccgctg gaacaggacg aacgtatcac cgcgatcctg
     6841 ccggtgaccg agtttgaaga aggcgtgaaa gtcttcatgg cgaccgctaa cggtaccgtg
     6901 aagaaaactg tcctcaccga attcaaccgt ctgcgtaccg ccggtaaagt ggcgatcaaa
     6961 ctggttgaag gcgatgagct gatcggcgtt gacctgacca gcggcgaaga cgaagtaatg
     7021 ctgttctccg ctgaaggtaa agtggtgcgc tttaaagagt cttctgtccg tgcgatgggc
     7081 tgcaacacca ccggtgttcg tggtattcgt ttaggcgaag gcgataaagt cgtctctctg
     7141 atcgtgcctc gtggcgatgg cgcaatcctc accgcaacgc agaacggtta tggtaaacgt
     7201 accgcagtgg cggaataccc aaccaagtcg cgtgcgacga aaggggttat ctccatcaag
     7261 gttaccgaac gtaacggttt agtggttggc gcggtgcagg tagatgactg cgaccagatc
     7321 atgatgatca ccgatgccgg tacgctggta cgtactcgcg tttcggaaat cagcatcgta
     7381 ggccgtaaca cccagggcgt aatccttatc cgtacttcgg aagatgaaaa cgtggtgggt
     7441 ctgcaacgtg ttgctgaacc ggttgacgag gaagatctgg ataccatcga cggcagtgcc
     7501 gcggaagggg acgatgaaat cgctccggaa gtggacgttg acgacgagcc agaagaagaa
     7561 taatttcatc gcttcatgcc aaaagggagc catctccctt ttttgaattg aaaagtccag
     7621 gctgtaaagt ctgggctttt gtcgtattag ggcgcggtaa agtttggctg tgcccgtaaa
     7681 aaatggctgg ctatacacaa ggaatgtggc aatgagtggt gaaaaaaagg cgaaaggctg
     7741 gcggttttat ggccttgttg gttttggggc aatagcgctg ctatccgctg gcgtatgggc
     7801 attacaatac tctggcagtg ggccagaaaa aacgttgtcg ccactggtgg tgcacaacaa
     7861 tctgcaaatc gatctcaatg agccggacct ctttctcgac agcgactctc tgagccagct
     7921 tcccaacgat ctccttacca ttccgtttct ccacgatgtt ctgagcgaag atttcgtttt
     7981 ctattatcag aatcatgccg atcgtctggg cattgaaggc agcattcgcc gcattgtcta
     8041 tgaacacgat ctcacgctga aagataagct cttttcgtca ctcttagacc agcccgcgca
     8101 ggcggcactg tggcacgata aacaaggcca tctttctcat tatatggtgc tgatccagcg
     8161 tagtggttta agcaaactgc tggagccatt gctgtttgcc gccaccagcg acagccagtt
     8221 aagcaagacg gaaattagta gcatcaagat aaatagtgaa actattcctg tttatcagtt
     8281 gcgctataac ggcaataacg tcctgatgtt tgcgacttat caggacaaaa tattggcgtt
     8341 ttccagttcg gatatgctgt tcaaagatga ccaacaggat accgaagcca cggcgatcgc
     8401 aagtgatttg ttgagtggta aaaaacgctg ggaaacccgc tttggcctgg aagcgcgtac
     8461 tgccgaaaaa acgccagtac gccagcgcat cgtagtcagc gccaggttgc tggggtttgg
     8521 ctaccagcga ttaatgcctt cttttgctgg cgtacgcttc gaaatgggta acgacggctg
     8581 gcacagtttt ctggcgttaa acgatgaatc cgccagcgta gatgccagtt tcgattttac
     8641 gccggtatgg aacagtatgc ctgccggagc cagcttctgt gtggcggtgc cgtattcaca
     8701 cggtattgcc gaagagatgc tttcgcacat cagccaggaa aacgacaagc tgaatggggc
     8761 gttagacggt ggcgcggggc tgtgctggta tgaagactca aaattgcaaa ccccactgtt
     8821 tgttggtcag tttgatggca ctgccgaaca ggcgcaattg ccagggaaac tgtttacgca
     8881 aaatattggt gcgcacgaaa gcaaagcgcc agaaggtgtt ttgccggtaa gccagactca
     8941 gcagggcgaa gcgcaaatct ggcgtcgcga agtgagttcc cgatacggtc agtatccgaa
     9001 agcgcaggcg gcgcaacccg atcaattaat gtcggattat tttttccgcg tgtcgctggc
     9061 gatgcaaaac aaaacgctgc ttttctccct cgatgacacg ctggttaata acgccctgca
     9121 agcactgaat aaaacccgcc cggcaatggt ggatgtaata cccactgatg gcatcgttcc
     9181 gctctatatc aatccacaag gaatggcgaa actgctgcgt aacgaaacgc tgaccagtct
     9241 gccgaagaat ctcgaaccgg ttttttataa cgccgcacaa actttattaa tgccgaagct
     9301 ggacgcttta tctcaacaac cgcgttatgt catgaagctg gcccagatgg aacccggtgc
     9361 cgcctggcag tggctaccca taacctggca accgctatga gacacgggct gctggtgctg
     9421 atttgctggc tgtattgtgt tgttgcccat agtgaaatgc taaatgtcga acaatccggg
     9481 ctgtttcgcg cgtggtttgt gcgcattgca caggaacagc tccgccaggg gccaagccca
     9541 cgctggtatc aacaggattg cgcgggcctg gtgcgatttg cggcgaacga ggcgctgaaa
     9601 gttcacgaca gtaaatggct aaaaagtaac ggtttatcta accagtattt gccgccagag
     9661 atgacgctaa cacctgaaca gcgtcaactg gcgcaaaact ggaatcaggg gaatgggaaa
     9721 accggcccct atgtgaccgc gattaatttg attcagtaca acagccagtt tattggccag
     9781 gacataaacc aggcactgcc tggcgatatg atttttttcg atcagggcga tgcccagcac
     9841 ttaatggtct ggatggggcg ttacatcatc taccacaccg gaagcgccac gaaaactgac
     9901 aacggaatgc gcgcagtcag tctgcaacaa cttatgacat ggaaggacac ccgatggata
     9961 cccaacgatt ccaatcccaa tttcattggc atttatcgtt taaattttct ggcgcgatag
    10021 cagcgtgctt gtccctgtat cttgtgggaa cagggcttac taatgctgat gattcgcttc
    10081 cttccagtaa ctatgtgccg cccgccgggg gaacattctt tttgctcgct gacagcagtt
    10141 ttagcagcag tgaagaggcg aaagtgcgac tggaagcgcc ggggcgtgat tatcggcgct
    10201 atcagatgga agagtacagc ggcgtggacg ttcgcctgta tcgtattcct gacccgatgg
    10261 cttttttgcg ccagcagaaa aacctgcatc gcattgtggt gcaaccgcaa tatctgggcg
    10321 acggactgaa caatacgctg acctggctgt gggataactg gtacggcaaa tctcgccgcg
    10381 tgatgcagcg tactttctct tctcagtcac ggcagaatgt gactcaggcg ttacccgaat
    10441 tacagctcgg taatgccatt attaaacctt cccgttatgt acagaacaac cagttttcac
    10501 cgctgaaaaa atatccacta gtggaacagt tccgttaccc actatggcag gctaaaccgg
    10561 tcgagccgca acaaggggta aaactggaag gcgcatccag caatttcatt tcgccgcagc
    10621 cgggcaacat ctatattcct ctcggcaaac aagagccggg actgtacctc gtcgaggcga
    10681 tggtgggtgg gtatcgggcg acgacggtgg tgtttgtttc cgataccgtg gcgcttagca
    10741 aagtgtcagg taacgagctt ctggtgtgga ccgcggggaa aaaacagggt gaagcgaagc
    10801 ccggctcaga gatcttgtgg actgacggtc ttggcgtgat gacccgcggt gtgaccgatg
    10861 gcagcagtac cttgcagtta caacatatat cgccagaacg ttcatatatt ctgggtaagg
    10921 atgctgaagg cggcgttttt gtctccgaga actttttctt cgaaagcgaa atctacaaca
    10981 cccgcttgta tatctttacc gatcgcccgc tatatcgcgc aggcgatcgt gtcgatgtta
    11041 aagtgatggg ccgcgagttc cacgatccgt tgcattcatc ccctatcgtc agcgcaccgg
    11101 cgaaactttc agtgctggat gctaatggca gcctgttgca aaccgtcaat gtcacgctgg
    11161 atgcgcgtaa tggcgggcag gggagtttcc gcctgccaga aaatgccgtc gccggaggtt
    11221 atgagttacg tcttgcttac cgcaaacagg tctatagcag cagttttcgc gtggcaaact
    11281 acatcaaacc ccattttgag attggtttag ctctcgataa aaaagagttc aaaactggcg
    11341 aagcggtcag cggcaaactg caactgctct acccggatgg cgagccggta aaaaatgccc
    11401 gcgtgcagtt aagtttgcgc gctcagcaat tatcaatggt tggtaacgat ttgcgttatg
    11461 caggacgttt cccggtgtcg ctggaaggca gtgaaacggt ttccgacgat aatggtcatg
    11521 tggcgttaaa tctccccgcc gccgataaac caagccgtta tttgttaact gtctccgcca
    11581 gtgacggcgc ggcgtatcgt gtcaccacca ccaaagagat cctcattgaa cgcggtctgg
    11641 cgcattactc tttaagtact gctgcacaat acagtaatag cggtgaatcg gttgtgttcc
    11701 gttatgccgc gctggaatct tcaaaacagg ttcctgttac ttatgaatgg ttgcgtctcg
    11761 aagaccgcac gagccacagc ggagagctac agtcaggcgg caaatccttt accgtcaatt
    11821 tcgctaaacc tggcaactac aatctgacat tacgcgataa agacggctta attctcgccg
    11881 ggttaagcca tgcagtcagc ggtaagggca gcacgtcgca tactggtacg gtagatatcg
    11941 tggcagataa aacactgtat cagcctggcg aaaccgcgaa gatgctgatt accttcccgg
    12001 agccaattga cgaagcatta ttgacgctgg aacgcgatcg cgtggaacag cagtcgctgc
    12061 tatcgcatcc ggcaaactgg ctaacgctac aacgtttaaa cgatacccag tatgaagccc
    12121 gcgtaccggt gagcaattcc tttgcgccta acatcacttt ttcggtgctg tatacccgta
    12181 acggtcagta cagttttcag aacgccggga ttaaagttgc cgttccccag ctggatatcc
    12241 gggtgaaaac ggacaaaacc cattaccagc ctggtgaact ggtcaatgtc gaattaacct
    12301 cgtcgctgaa aggtaaacct gtttctgcgc agttaacggt aggcgtggtc gatgaaatga
    12361 tctacgcgct gcaaccggaa atcgcgccga atatcggcaa atttttctat ccgctggggc
    12421 gtaacaatgt gcgtaccagc tccagtctgt cgtttatcag ctacgatcag gcgctctcca
    12481 gcgagccggt tgcgcctggc gcgactaacc gcagcgagcg gcgagtaaaa atgctggaac
    12541 gtccacggcg tgaagaggtg gataccgcgg cctggatgcc gtcactcaca accgataaac
    12601 aaggcaaagc gtatttcacg ttcctgatgc ctgattcgtt aacccgctgg cgcatcaccg
    12661 cgcgtgggat gaacggcgac gggctggtcg ggcaggggcg tgcttatctg cgttcggaaa
    12721 aaagtctcta catgaaatgg agtatgccaa cggtgtatcg catgggcgac aaaccggcgg
    12781 caggactgtt tatcttcagt cagcaggata acgaaccggt ggcgctggtg actaaatttg
    12841 caggtgctga gatgcgccag acgctgacgc tgcacaaagg ggcgaattat atttcgctga
    12901 cgcagaacat tcagcaatct ggcttgttaa gcgcagaact gcaacaaaat gggcaagtgc
    12961 aggacagcat tagcacaaaa ctgtcttttg tggataacag ctggcccgtt gaacagcaga
    13021 aaaacgtcat gctcggcggt ggcgataacg cgctgatgtt gcccgagcag gcgagcaata
    13081 tccggttgca aagtagcgaa acgccgcagg agattttccg taacaatctt gatgcgttag
    13141 tcgatgaacc gtggggtggc gtgatcaaca ccggtagccg tctgatcccg ctcagtctcg
    13201 cctggcgttc gcttgccgat catcaaagtg ccgccgctaa cgacattcgt cagatgattc
    13261 aggataaccg tctgcggctg atgcaactgg cggggcccgg agcgcgcttt acctggtggg
    13321 gtgaagatgg caatggcgac gccttcctca cggcatgggc atggtacgcc gactggcagg
    13381 ccagccaggc gctcggcgta acgcagcaac cggaatactg gcaacatatg ctcgacagtt
    13441 atgccgagca ggcggacaac atgccgttat tgcatcgggc gctggtgctg gcgtgggcac
    13501 aggagatgaa tctgccgtgc aaaacgttgt tgaaagggtt ggatgaagct atcgcccggc
    13561 gcggaactaa aactaaagat ttctctgagg cagacaccag cgatatcaat gacagcatga
    13621 tcctcgatac acctgaatct ccactggcag atgcggtggc aaacgtctta accatgacgt
    13681 tgctgaaaaa agcgcagttg aagtccactg tgatgccaca ggttcagcaa tatgcgtggg
    13741 ataaagcggc aaacagcaat cagccgctgg cgcacacggt tgtgctgctc aatagcgggg
    13801 gcgacgctac ccaggcggct gctattttaa gtggtttgac cgctgagcaa tctactattg
    13861 agcgcgcgct ggccatgaac tggctggcga aatatatggc gacaatgcct ccagttgttt
    13921 tgcctgcgcc tgcgggcgca tgggccaaac ataagttaac tggagggggc gaatactggc
    13981 gttgggttgg tcagggcgtg ccggacattc tctcttttgg tgacgaatta tcgccgcaaa
    14041 atgtgcaggt ccgctggcgt gaaccggcaa aaacggctca acaaagtaac attccggtga
    14101 ccgttgaacg ccagttgtat cggcttatcc ccggtgaaga agagatgagc tttactctgc
    14161 agccggtgac cagcaatgag attgacagcg atgcgctgta tctcgatgaa attacgctta
    14221 ccagcgagca ggatgcagtt ctgcgctatg gtcaggtgga agtaccgctc ccgccgggag
    14281 ccgacgttga gcgcacaaca tggggcattt cggtcaataa acctaacgct ggaaaacagc
    14341 aggggcaatt gctggaaaaa gcgcgtaatg aaatgggcga actggcctat atggtgccgg
    14401 tgaaagaact gacgggaacg gtcactttcc gccatttgct gcgcttctcg caaaaagggc
    14461 aattcgttct gcctcctgct cgttatgtgc gttcctatgc acctgcacag caaagtgttg
    14521 cggcagggag tgaatggacc gggatgcagg tgaaataagt gaactggtac agaatcgtct
    14581 ggctattggc gttggttact ctgccaacgc tggcggagga aacgccgtta caactggcgc
    14641 tgcgcggtgc gcaacacgat caactgtatc agctgtcgtc atcaggcgtg accaaagtct
    14701 cggcattacc tgacacgctc accacgccat tagggagttt gtggaaactg tatgtttacg
    14761 cctggctgga ggatacccat caaccggagc agccctacca gtgtcgcgga aactccccgg
    14821 aagaggttta ttgttgtcag gctggggaga gcataacgcg cgataccgcg ctggtacgct
    14881 cctgtggact ctattttgcg ccgcagcggt tgcatattgg cgcagatatg tgggggcaat
    14941 actggcaaca gcgtcaggca cctgcgtggt tagcgtctct tactacactg aagccagaaa
    15001 cctcggtaac ggttaaatcg ctgcttgatt cattagcgac attacctgcg caaaacaaag
    15061 ctcaggaagt cttactggat gtggtgctgg acgaggcgaa aattggtgtc gcatccatgc
    15121 tcggtagtcg ggtacgggtg aaaacctgga gttggtttgc cgatgataag caggaaatcc
    15181 gtcagggtgg ttttgctggc tggttaaccg atggtacacc gctgtgggtt actggcagtg
    15241 gcaccagcaa aacggtactg acccgttatg cgacggcgct taatcgggtt ttgccagtgc
    15301 cgacgcaggt tgccagtggg caatgtgtgt tggtggatct gtttgctcgt tatccgctga
    15361 agaaagttac tgaggaaaag agtacgacgg cagtcaaacc aggcgtgctc aatgggcgct
    15421 atcgtgtgac gtttgccaat ggtaaccaca tgacatttgt cagccacggt gagacaacgt
    15481 tactgacggt aaagggcaaa ctgaaattac agtcgcatct cgatcgtgaa gagtacgttg
    15541 ccagagttct ggatcgcgag gcgaaaagta cgcctcctga ggcagcgaaa gccatgaccg
    15601 tggcgattcg cacgtattta cagcaaaacg ccgatcgcga tggtgattgc ctgagcatcc
    15661 cggacagtag cgctacccag cgcgtttcag cttcgccagc cactgtaggc gcgcggacga
    15721 tgactgcctg gacgcaggat ctcatctatg cgggcgatcc ggttcattat catggcagcc
    15781 gggttaccga agggacgctt tcctggcggc acgctactgc ccaggcgggg cagggagagc
    15841 gttatgatca gatacttgcg tttgcttatc ccgacaacaa ccttagccgc tggggagcgc
    15901 cacgctcaac ctgtcagtta ttacccaaag cgaaagcctg gctggcgaaa aaaatgccgc
    15961 agtggcggcg cgtgttacaa gatgaaatgg ggtataacga acctgacgtg tttgcggtct
    16021 gccgcttagt ctcaggtttc ccctataccg atcggcagca gaaacggctg tttattcgta
    16081 acttctttac gcttcaggat cggcttgatt taacccatga gtatctgcac ctggcctttg
    16141 atggttatcc caccgggctt gatgagaact atatcgaaac gctgacccga caattattaa
    16201 tggactaatg ctatgcgaaa gatttttctt ccgttgttac tggtggcgct ttcgcctgtc
    16261 gctcacagtg aaggtgtgca ggaagtcgag attgatgcac cgctctctgg ctggcatccg
    16321 gcagaaggtg aagatgccag tttttcacaa acgatcaatt acccggcgtc gtccgtcaac
    16381 atggcagatg atcaaaatat ctcagcgcag atccgcggca aaataaagaa ttatgctgcg
    16441 gcgggtaaag ttcagcaggg ccggctggtg gtcaacggtg ccagtatgcc gcagagaatt
    16501 gaatcagatg gttcttttgc acgcccttat atcttcactg aaggcagcaa cagcgtgcag
    16561 gtcatcagcc cagatgggca aagccgacaa aaaatgcagt tttactcaac gccgggtgct
    16621 ggggcgattc gcgcacgttt acggctggtt ctctcgtggg atacggacaa taccgacctc
    16681 gatcttcatg tcgttacgcc tgacggtgaa catgcctggt acggtaacac cgtgctgaaa
    16741 aacagtggtg cactggatat ggatgtcacg acagggtacg gccccgagat tttcgccatg
    16801 ccagcgccag ttcacggccg ttatcaggtg tatatcaact actatggcgg acgcagcgaa
    16861 acggaattaa ccaccgccca actgacgctg atcaccgatg aagggtcggt caatgagaaa
    16921 caggaaactt ttattgtacc gatgcgtaat gctggcgaac tgacgctggt gaaaagtttt
    16981 gactggtgat taatgaggtt atctttactg tcaatgttca agaaaacgcc cgatgacttt
    17041 cgctatcggg cgtttttaca tgttaattca atcgttcaat caccatcgca attccctggc
    17101 cgccaccaat gcacagtgtt gccagcccca gcgttttatc gcgtgcctgc attgcatgta
    17161 atagtgtgac cagaatacga gcaccactgg caccgatagg atgccccagc gcgatggccc
    17221 cgccgttgac attcactttc tcaggctcaa agcccagggt tttcccaacg gcaaggaact
    17281 gtgcagcaaa tgcttcatta gcctcaatga gatcaatatc cgccacttgc agccccgcca
    17341 gttgtaacgc tttttgcgtg gcaggtactg gccccatacc catcaatgcg gggggcacgc
    17401 caccgctggc ataactttta atgcgtgcca ggggattcag gcctgctgcc agcgccgcag
    17461 attcttccat aatcaccaga gcggcagcac cgtcgttaat acctgacgcg ttcccagcgg
    17521 tgactgttcc tgctttatcg aaggccggac gcaatgcgcc taacgcttca gtcgtagaat
    17581 ccgctttcgg gaattcgtct tgactgaaga cgaaggtttt cttccgggtg acaacattta
    17641 ccgggacgat ttcggctgta aaagcaccgg actcaattgc ggctgccgct ttacgctgtg
    17701 aatgtagcgc cagttcatcc tgcatttctc gggtaattcc gtactctttg gccacgttct
    17761 cggcggtaat ccccatatga taaccatggg tggcgcacat cagaccatcg cgcaggatta
    17821 cgtcataaac ctgtccgtct ccaagacgat aaccagagcg tgcttttgca tcgagtaagt
    17881 agggcgctaa actcatattt tccatacccc ccgccacaat gctttgcgcc tgaccagcct
    17941 gaattgcctg ggtggcaagc gccacacttt taagacccga accacacact ttattgaccg
    18001 tgaatccgca caccttttct gccagcccgc tttttaacag tgcctgacgt gccggatttt
    18061 gccccaaccc ggcttgcaac acgttaccca taatcacttc atcaatgtgt agtgaatcga
    18121 tttttgcacg ttcaatggcg gctttaatta ctgtcgcccc caggtcgatg gcgctggtgg
    18181 aagcgagtga accgttaaaa ctaccgatag cagtacgtac cgcactgacg atgacacaat
    18241 ttttcatttt ttattccttc tgtctgtagg gttgccgtca gaacagcgtt aaaccaatga
    18301 cgaaaatgac gccggagaag agcagggcag tgatgcaata gcccatgatg tcgcggacac
    18361 cgagtccggc gattgccagt gctggcagcg cccagaatgg ttgtgccatg ttcatccatt
    18421 gctcgccgta ggcgatcgcc attaccgatt taccgagatc agcgcccagc gcctgggctg
    18481 cgggtatcac gaaaggtccc tgaataaccc agtgaccgcc gccagacgga acggcgaagt
    18541 taatcagtgc agaactaaaa aaggtcatta ccgggaaggt gtctttgttc gcaacattga
    18601 tgaagaattc ggtaatgagt ccgcccagac cggaatgctc catcatcagt tggatccctg
    18661 cgtagaaggg gaattgcacc agaataccgg cagtactgcg tgctgccgcg ctgatagcac
    18721 gcatataagc cattggcgtt ttatgtagca gcagacccgc aatcataaac atcaggttga
    18781 cggtattgat ggtgatgttg aagccatgtt cgctgaagta catcgccagg taagcgatac
    18841 cgagtgcgcc gatgatcaac gtcagaatac ggctttcttc cagtcgctcg gacggtggag
    18901 catctttcgg tagttgcttt tgaaaatcag cctcttccat gagtagtttc ggatcgatac
    18961 tcaccacgtc agacggtttt ggcatcatca tgcgggtgat aaatggcatc accacaatca
    19021 acgccacagt gatgaaaata ttaaaaccac tgaacagagt atcgcccacc gggatcagcc
    19081 cggcgatatg ctcaaccggg ttgcccggtg ttgcagccaa cagaggcatt gagccagaga
    19141 aaccgccacc ccaggtgaga aaaccaatgt aggcgcaggc aatgagcaac ggataatcag
    19201 aaccgggtac tcgccgggcg acttcacggg cgaacattgc accgacaacc aaaccaaatc
    19261 cccagttgat gacacaagcg actgaaccga agaaagtaac cagcatgacg ccctgtacgg
    19321 gcgtctttgc ggcggaggcg gcagtacgca gcaaactttt caccggcgcg gagctggcaa
    19381 gggcatgacc ggtaacgatg ataagcgcca tctgcatacc aaacgccagc aagttccaga
    19441 aaccgtcacc ccacattttc accatgctga tcggcgtttg tggtgttaac caaagcgcga
    19501 tcacgaatgt tagcaatgtc agcaacatgg caaagatcag tggatcggga agccaccggc
    19561 tgacaaaacg cgtcataaaa cgcgatatgc gaccaatcat aaatcacccc gttgcgtatt
    19621 cagatcggca gcgacttcaa accgagcttc tgttttggca cgcacggtgg ctaaatcaca
    19681 cccgtcggca atttcggtga gccacatttt gccgtcaata aaacgaaaga cagccagttc
    19741 agtaaccagc atatgcaccg catgttgcgc agtgagtggc atggtgcagc ggcgcaaaat
    19801 ttttgctgaa ccatctttgg cgcaatgttc catggcgatg atcactttgc gcgacccggt
    19861 caccagatcc atcgcgccac ccataccggg caccattttc ccaggcacta cccagttcgc
    19921 gaggtttgct tcttcgtcta cttgcaaacc gccgagcacg caggcatcaa tatgaccgcc
    19981 acggattagc gcaaatgaca tggcgctatc aaacatggct gcaccgggta aaatgccgca
    20041 cggttgtccg ccagcgttca ccagatcggg atgcgctgtt gtgaccgggc ctaaaccgag
    20101 gaagccgttt tccgattgca gagtgatatg aataccctcc ggtaaataat tggcgaccat
    20161 tgtgggtaaa ccgattccta agttaacgat gtcaccatca cgaagctctt gcgccacacg
    20221 gcgcgcaata cgttgtttcg catccattat ttgctctcct gtgaaacgat gatgtggtcg
    20281 ataacggcac caggggtgac aatatggtca ggttgcagct cgccggtttc gaccagttca
    20341 tccggctcta ccagcgtgat atcagccgca agggctatca gggggttaaa gttgcgggcg
    20401 ctaaggtgat aggtcaggtt gccaagtgtg tcgcaacgat gagcgcgaat tagcgccagg
    20461 tcggcgcgca gcgggcgttc gagcagccag gttttaccgt cgagtgtcag tgtctgtttg
    20521 ccttcctcta cgacggtgcc gacaccagtt ggggtgagaa aaccaccaag tccagctccg
    20581 ccacagcgaa tttgctcgat tagcgtacct tgcggcacca gaacgacgtc catctcacca
    20641 gatatcatgc gccgacctgt ttccgggttg gtgccgatat gtgaagcaat cactttgcgg
    20701 actcgaccat tgacgatgag cggaccgatg cctgtatcaa caaacgcggt atcattggct
    20761 atcaatgtca ggtcgcgaac accagattcc agtaatgctt caaccaggcg ggatggagtg
    20821 ccaatcccca taaatccgcc caccatgatg gtcatgccgt cacgaaagaa gccggtggcg
    20881 tcttgtaatg tcatcaattt tgttttcatt ttttatccct cttgcataca ccgttgtggg
    20941 tacagtcagg gaatagcaag gggagtgcca gaatgagatt attgaatgtt gttatttaac
    21001 taattgaata gaaaaggata aattagatgg ggattcataa ggagaatgag cggctatgca
    21061 gaaaattgcg cactgtgcaa atttctgcat agcaagtttt ggcgttatac atccgccgga
    21121 tcgataccgt attcctggag tttatacatc aatgcacggc gactgatgcc cagcattaaa
    21181 gcagtgcggg ttcggtttcc ttcttgttgt tccagcactt ccataatgat gcgtttttcg
    21241 acgcgtttaa tttcctcttt taaattacgc tcaccgacag gggctgtttt tgcttcacca
    21301 gcattacaga ctggctgacg aatctgcggc ggaagatcct cagaaaaaat gatcgggcct
    21361 gaattcatca cgacggcgcg ttcaataacg ttggaaagct ctcgaatatt tcccggccat
    21421 gaccaggcgg taagcagtga cattgccatc ggatcgatgt cgataatatc gcgctgattc
    21481 tcactactga atttttgcaa aaagtgatta gctaacaggg aaatatcttc ccggcgatcg
    21541 cgcagaggcg gcagtattaa atgaataacg ttaaggcgat aaaagagatc ttcacggaag
    21601 gtgccttctt tcaccattgc ctgcaagtcg cggttggtgg cagcaatgat gcggatatca
    21661 acttttatgg tctgatggcc gccaatccgt tcaaattccc gttcctgcag aatgcgtagt
    21721 aatttggctt gcagtaccag cggcatttcg ccaatttcgt cgaggagcag agtaccttcg
    21781 ttggcacgtt caaataatcc ctgacgcaag gtttgtgcac cagtaaatgc acctttttca
    21841 tgaccaaaca gttcactttc gagcaacgat tccggcagcg ccgcgcagtt gactttaatg
    21901 aatggcccct ttgcccgccg cgaattgtag tgaatcgctc tggcaatcaa ctctttccca
    21961 gtgccgcttt caccgctaat caagacgctg gcctgagaaa gggcaatttt ggcggtgtct
    22021 ttgcagatgt ccatcatcgc cgggctgttg gtgagaatgt gcccccattg ccagctggtg
    22081 ctcagtgcct ggtgcagatg acggatctct tttttcattg actggagttg taaagcgcgc
    22141 tgaacgatta aattcaactc atcgagatca aacggtttga taacatagtc gaaggctccg
    22201 cagcgtagtg cttcgacggc ggtttccact tcagcatagg ccgtcatcag aataacgggt
    22261 gtccgggtct catggctgcg catctccttt agtgccttga tgccgtccat ctctggcatg
    22321 cggatatcca tcaacaccac gtcagggtga atatcggcaa acaggtgtaa tgctgtgcgc
    22381 ccgttgttcg cacaatgtgt ttcgaatcct tgtagtgcaa aagcggtgct cagcatacgg
    22441 cgaacattat cttcatcatc cacaataagg atgcgattaa tagcagtcat acagtctgat
    22501 ttccctgcgg gttgatcggt aaaataagcg tgaaggttgc gccgtagccc ggcaaactgg
    22561 cgacgcgaat atcaccctga tgggcattaa tgatgcgttg acttaacgcc agaccaagcc
    22621 cggttcctga ggctttggta gtgaaaaagg gatcgaagat ctttttttgc agcgagagat
    22681 caatgccact gccgttgtcc tctatcgaaa tggcctgttg cgagtcgcta tactgccagg
    22741 ttcgaatgcg aattttccct cgtgcactga tagcctggac ggcattgatc aggatattca
    22801 gtagtacctg tttgagcagt tcacgatcgg cgttaatcgg gcttaattca ttatccagtt
    22861 cgcttatgaa gtcgacccgc gcttgtacgc cggcggtttg taccagtacc agagtttctt
    22921 caaccaatgc attgaggctg acttgttgcc attgactgtg gcgtggacgt gaaaattcga
    22981 gcaattgctg aataactttg ttaattgaat cgatttcttt gagtactacg gacagatatt
    23041 cctgatgtat tgggtcacgg gtttgttggc gcaagatctg tacataacca cgaatagctg
    23101 ttagcggatt acgtacttca tgcgccacgc cagccatcag ctcacccagt gtggcgaggc
    23161 gttctgcttg cgccatgcgg cgctgggttt ctttgcgggc agttaaatca gagaaaatca
    23221 ccaaagcacc tatcatttca ccgtgcgtgt tatgaatacg actggtcgtg acactgagtt
    23281 caatggtgcg gtcacggcct ggaaaactga tctccagtgc tacatgttcg gtgccatgtt
    23341 ccagcgtatc cagtactgga ctgtagaact gagtattgtc gaacaacatg gagtaaggct
    23401 gccctaccag ttcatgacgt tgatagccag tgatgacttc tgctgctggg ttcatggtgg
    23461 ttacatcacc ctggcggtca atggcaatga cgccatcggc agcgttttca ataatcagat
    23521 cgttaagtgt tcgcgtttca cgcagtgcct gggcgaggtt gttaacactc tgactgattt
    23581 gccccatttc accgggcaat tgtggtaatc gagtgggaat attttgtgcc agagtcgaga
    23641 ggccatcggt gatgatatct atattggcgc tcaggcgacg ggagaaaagg acaatcaaca
    23701 gcaggcttat cagcaaaccg gcggtgagca caatgataat cctcacatcc attttccagg
    23761 cctggcggcg aatatcttcg gttaattcat tggcccagat atagccgagg atttcaccat
    23821 tacgctcaat gggaatcatt gaattcaaaa tatcgccgcg cacctgcctg cctgaataaa
    23881 ccaaaggggt atttgtacgc atgacttcgc gaccagggtg atctgcggca atggtaacgc
    23941 ccacattatt ctgatatagc gctgaaggcg cgtaggtgat tatcgcatcc agcgttttgt
    24001 tgtaataacc agcgccgatg ccagggaagg cgtgagtgat attttcggta atgggggcaa
    24061 gttctgcatt taatgcgcgg atacgctcct cacgcggtaa gtcgatgtag agatcgtagc
    24121 gattgcctag cgcctgatta agcaggttga ccacggcaga aagttttttc tctttttcag
    24181 ataagactgc tgaacgtcct tccgtttcta cgatataacc aatagtaagc gttgggacaa
    24241 tgaccatcag gattgccatc aggatcattt gattgcgtaa gcggcgtgga taaatccact
    24301 tcatataatg catgctgaaa tccactagtc ttgtccggta tatgacgatt atcagaggtt
    24361 aaggtgatga tttctcggcg gtgtatcata ttccagagaa gagagaacat tgcggtaaca
    24421 cgcttttacc gctaccttaa ccacactcca tcggtcacct gaggcggagc ttcgcccctt
    24481 tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg ttcagagcat
    24541 tggcgttagt gctctggctg ttgattgctt tttcatccgt tttttacatc gttaatgcgt
    24601 tacatcagcg agaatcggaa attcgtcagg aatttaatct gagttccgat caggctcagc
    24661 gctttattca acgcacctct gatgtgatga aagagctgaa gtacatagct gaaaatcgct
    24721 tatcggcaga aaacggtgtg ctttccccgc gtggacgaga aacgcagacg gatgtgcctg
    24781 cgtttgaacc gctatttgct gactctgatt gttccgcaat gagtaacacc tggcgaggtt
    24841 ctctggagtc attggcgtgg tttatgcgct actggcgcga taatttttct gcggcttacg
    24901 atctcaaccg ggtattttta atcggcagcg ataacctctg catggccaat ttcggtctgc
    24961 gtgatatgcc agtggaacgc gataccgcgc taaaagcttt gcatgaacgc attaataaat
    25021 atcgaaatgc accacaagat gatagtggca gtaacctcta ctggatcagc gaaggtccgc
    25081 gccctggcgt cgggtatttt tacgcgttga cgcctgttta tctggcgaac cggttgcagg
    25141 cgcttttggg tgtcgagcag accatccgga tggagaactt tttcttacct ggtacgttgc
    25201 cgatgggggt taccattctt gatgaaaatg gtcataccct gatttcgctt accggaccag
    25261 aaagcaaaat taagggcgat cctcgctgga tgcaggaacg ctcctggttt ggctatacgg
    25321 aagggttccg ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta agcatcgttt
    25381 attcagtgcc ggttgataag gtgctggaac gcattcgcat gttgatcctt aacgcaattt
    25441 tgctgaatgt gcttgccgga gctgcattgt ttactctcgc gcggatgtac gagcggcgta
    25501 ttttcattcc ggcggaaagc gacgccctgc gactggaaga acatgagcag tttaatcgca
    25561 agattgtcgc ctccgcgcca gtgggtatct gcattttgcg taccgctgat ggcgtcaata
    25621 ttttaagtaa cgaactggcg catacctatc tcaatatgct tacgcatgag gaccgccaac
    25681 ggctgacaca aattatctgt gggcagcagg tcaattttgt tgatgtcctg accagcaaca
    25741 ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac gtggccattt
    25801 gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag gagatggcac
    25861 aagcagcgga acaggcgagc cagtcaaaat cgatgttcct ggccaccgtc agtcatgagc
    25921 tgcgaacgcc gctgtatggc attatcggta acctggatct gttgcaaacc aaagagttac
    25981 cgaaaggcgt cgatcgtctg gtgacggcaa tgaacaactc ttccagcctg ttgttgaaaa
    26041 ttatcagcga tattctcgat ttctcgaaaa ttgaatcaga acagttgaag atcgaaccgc
    26101 gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc caactattta ccgctggtgg
    26161 tacgcaagca gttaggcttg tactgcttta ttgaaccgga tgtgccagtg gccttaaatg
    26221 gcgacccgat gcgtttacag caggtcatct ccaacctgtt gagtaacgcc ataaaattca
    26281 ccgataccgg ctgtatagtt ttgcatgttc gcgctgatgg cgattatctc tctatccgtg
    26341 ttcgcgatac cggcgtgggg ataccggcga aagaagtggt gcgcttgttt gatcccttct
    26401 tccaggtcgg aacgggcgta cagcgtaact tccaggggac tggtctgggt ctggcgattt
    26461 gtgaaaaact gatcagcatg atggacggcg acatctcggt agattcagaa ccgggaatgg
    26521 gcagccagtt taccgtgcgt attccgttgt acggcgctca gtacccgcag aaaaaaggtg
    26581 tggaagggtt gagtggtaaa cgctgctggt tggcggtccg caatgcgtcg ctctgtcagt
    26641 tcctggaaac cagtttgcag cgcagcggca tcgtcgttac aacatacgaa gggcaggaac
    26701 cgactcccga agatgtgtta attactgacg aggtagtgaa caaaaaatgg cagggcagag
    26761 cggtagtgac cttctgtcgt cgccatattg gtattccgct ggagaaagcg ccaggggagt
    26821 gggtacacag tgtggctgct ccgcatgagc taccggcatt gttggcgcgt atttatttga
    26881 tcgagatgga gagcgacgat cctgctaacg ctctgccgtc gacggacaaa gcggtcagcg
    26941 ataatgacga tatgatgatt ctggtcgtag atgatcatcc gattaaccgg cgtctgctgg
    27001 cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc gaatgatggc gtcgatgcgc
    27061 ttaatgtact taacaagaat catattgata tcgtgcttag cgacgtcaat atgccaaata
    27121 tggatggtta ccgcttgacg caacgcattc gtcagttggg actgacgttg ccggtaatcg
    27181 gagtaactgc taatgcgttg gctgaagaga agcagcggtg tctggagtcc ggtatggaca
    27241 gctgcctgtc gaagccggta acgctggatg tgataaaaca gacgctgacg gtatatgccg
    27301 agagggtcag gaaatcgcgg gaatcgtagg acggataagg tgtttatgcc gcatctggca
    27361 ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc tggcctactt taatgtaatc
    27421 tgttgaaata atgggaatcg taggacggat aaggcgttta cgccgcatcc ggcaatccgt
    27481 gcaaatgcca gataagacac tgacgcgtct tatctggcct acaggtgatt agtctttatc
    27541 tgccggactt aaggtcacag aagagagata attcagcaga gcaatatcgt tctcgacacc
    27601 cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc ggttcagctt
    27661 tttagcgatc tcggtcacca ggaagccttc cgcaaacagg cgcagaactt cactctcttt
    27721 tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca ggcgagaaac
    27781 gctttccggg gtaaattttt tccctttctg cagcgcggcg agagccttcg gcaaatcggt
    27841 cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg cactaagaat
    27901 cgccgggttg ttgttcatgg tcagaacaat gatcgacagg cttgggaaat ggcgcttgat
    27961 gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga gatcggtaat
    28021 caacacatgt gcatccagtt tcggcaagtt gttgatcagt gctgtagagt cttcaaattc
    28081 gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga acaagactat
    28141 cggatggtca tcggcaataa ttacgttcat attgttcatg tacttgtacc tatgtattaa
    28201 ctatttgatt ttaaaaatat attctatgct ttgtttagtc gttggggcac ctgtgggaca
    28261 taagtacata gctttctgtt tagtacttct acctgggact ggttattgtc tttcatccat
    28321 gcaccgtaaa cattgtaaac catctgggca ttcgcatgtc ccatctggtt ggctatgaag
    28381 tttggatttg ctccggcagt taaagcccag caagcataag tatgccgaga ctgatacgct
    28441 tttctatggc gaatttttgc tcgttttaat gcagattccc atgttctgtt gatggatgtt
    28501 acggcataat gaactcctga gttacctgtg cgatctaata tctgaggatt gaaaacaaag
    28561 ctgcatgagt gagtgcttgt ccggccatac tctcgcagct ttacttctat ctgatattgt
    28621 ttaccaagcc tggttaatgt cgcctgattt tttaatacgt caattgctgg ctgtatgagt
    28681 tgtattaccc tgtttgttcc tgcatctgtt ttgggtagtg taaattcctt tgtctgagtg
    28741 taattccttt tgacggtcaa ggtacccgct tccagatcga tatcctccca tgccagccca
    28801 cacagttcgc catgccgcat cccggtatat acagccagcg accagaaatt tttagtttgc
    28861 tggtggtggc acgcatcaat cagtctggca aactcatctt ttgttagtgg atcgggaggt
    28921 gttttcgctc ttttcagtga tggaagctca ctgaaagggt ttttgcctat atacccattg
    28981 tttgccgcaa aacggaacat cccggagata attgtcatat agctatttac agtaggtaca
    29041 ctacgacctt taataggctt cctctgattt tttcctggca actgatatcc cgttaataat
    29101 tctttacgaa aaatgagaac atcttcctgt gtaattgatg atgccagttt gttccctcca
    29161 aggcagtcaa ggcaatttcg gatagctgat ttgtatctga ccatggcatt tgctgttatt
    29221 tccattttct taagctcaag ccatttttcc gaaagtgcct taatggttat ctcttttttt
    29281 cccagaccaa agtgtttcag gttaggggaa ttagggaact gcgcggcgta gtcgaagctc
    29341 cctattctga ttgcaaaaca aacggaagta cgaagttcac ctgcgatctt ccggtttttg
    29401 gcggtgtcag gaacaccgag gttttctctg acacgtttgc cgttatagtg aaaccatatg
    29461 cggagtgatc cgccatggtt ttcaacgcct gtcgggtatg atgcgttact cattaaacct
    29521 cccagacgtc caggagcatt aacaggttaa ccggaacttg catttttggc acctggttgt
    29581 ttctggtttt cgatccagcg cataatttcc tcaatgttgt atacacattc actgaaaggc
    29641 ccgggctctc cttctgctgc gtaatgacgg tattctttcc ccagtaacca ggattttttt
    29701 cttgctctgg caatggttcc tgatttcagt ccagttgcag caataagggt tctttctgtg
    29761 caccatttgc tgggcgttat ctgatagatg attgtttcca ttcctgactc acactatctt
    29821 caggccacgg cagtggcacc acacgtcaaa cattcgcttt acaacttcac ggcagtagaa
    29881 gccgtgagca tctctggtta aatcgtatcg gtacccgtat ctgagtcgta tccatatttc
    29941 aaactcgcga ttcacgactt attctcctgc tgtctttatg ccagttcatc cggttgcgct
    30001 ggggcccagg ccttccgtgg cctgtatttg cggcgttttc tggcttcaat gcgtatcttt
    30061 gtcaccctga ctggctttgg ttcgtgccgg aacactttgt ttctgatatt acgccgtgta
    30121 ttcatcatcc agataaaacg ctgggtatgg tcgcatccgt catcaacggt aattactgtg
    30181 cggaccagtt catcgttaat atcaaccatt tcacccattc actgagccct catacgcgaa
    30241 tttcgtggct tccatcagtt gcgtccatac ccattcaaga aattgtgcgt tttctcctga
    30301 gcggtcatat tcatcgacgc tgcggggctt gatggtcgcc agtagtggca caatccggta
    30361 cttcatctgt tcggttaact tgtccggtac aagtcgccag ccgttatatt tcgatggaac
    30421 atggtggtat tcagccagga agcgtcgtat atcgtttttc atttcctcgc atgagttctg
    30481 atcatgaagt gcagcggaaa gccagcgtcc gatatattca gggattcgca catcaagtac
    30541 tttttctgcc gtcgtcagca ttgcctgtcg gcaggcgttc cagccgttga catagtgacg
    30601 tgttgcatcc catgaaacct gcacatcagg gtaagcatcc tgttctgttc tcactggcgg
    30661 tatctgagga acagtgaata gtggactgac gatatattta tccagagagc ctccccgctc
    30721 ttctgcaaga ctttcccacg atgtcattgt ggggaccctg taacctgctt ttttgtgcgt
    30781 atacatccat gccactctgc cgttatcatc tgcggcggca atgcgctgcc aggcaggata
    30841 aagcggggtg tatgtttctc tgtcattacg ggcgtcaggc atggcatcaa tactgcaaac
    30901 ttcccccgta aggttattca gccaggcctt cgggctaatc tctgacgtta cgatttcatc
    30961 cagttttttc tccagatttt ctatgtgact gattagcgca aggataactg gcggagtggc
    31021 aagggagtga aatgcttcca gatcacatcc cttgtctcca tatacggtat tttgtgcggc
    31081 gtcacgcagt gctttcagat cagcttcttt catcgttcag actcccttgt tgctgttttg
    31141 taggcgcgca ggatgtcgcg tgtttttcct gataatgacg acttaaccag gaaaaagccg
    31201 ctccggcttt ctgtgatgtc cggcgtgcac agaagtaccg catcaacgac acgattatgt
    31261 tttcgcaatg tcaggcggga actggtgata atcagttttg cctgttcgcc gtaatcggtg
    31321 aatgaaataa tcatctgatg gcgatctccg ttatgaaagt gttcctgctg gttcaatgct
    31381 gataatttct gctgcaataa gcatacctgc cagccatgct tcgccgagca tgtcctcatc
    31441 cgtacatgca agatcaccga tctgtattgt ggccataatg ttagtttcac cggtatattc
    31501 atcctgtgct tcttcatacg gcaggttttc ataaaggttt tcaattgcgc aggttattac
    31561 atccagtccc gtcagattgc cgacaacgtc cacttcaaat gtttcgcggt actcccattg
    31621 cccgagtgtc agacgaaccg tctgtcttgc catgcgcccg cataatgtca tgttcgggtc
    31681 gtagttcatg attgccgcag tagtgctatt catgttctgc ctccggtgtg taaatagctt
    31741 tgtcgtgttt aaattcaccg ttccagtttt tcttcatagg gagttcaccg ttcatgtaaa
    31801 actggtataa acggtggcat cctttttcca gcaatacggg ggtgaatttc gtgaatgttg
    31861 ctttcccgtg tggagtgatc tgtactgctt cttcggtcag gtatctgtca cgggcataag
    31921 aggccgtgcg ccagcggggt gttttctccg gatcgcgttg ttcgttgaat acccattttc
    31981 ggccagacag ccaccacatt atctggctgg tatttacgcc gttcagcgca cggcagaact
    32041 ggggaacagt catgccttta gagaaatgtt tttccagact gtctacggtt gcagagagcg
    32101 ttttattttc cagggccata gcttcagccc gttcttcggc ctcaataacc atcaacgcga
    32161 gttccttgcg agtgagtacg cgctgaacat cgatgttttc ccgctgagtg aagtagaact
    32221 ccacaaggtc ttcgtgatag ttccacgcct gatccgtttc cagcaatttt gcgtggttgg
    32281 ctgcgccgcg ttcggtccag aggatgaggg atcgaacatt gctggcaatt ttcacagagt
    32341 agctttgaga tactctgtgc ttaagttcac gtagttcatc gccttccagc aaaaaataat
    32401 gctttccaac aacgaatcgt tcttcgtttc gggaatgatt ctggcgaata cggattggtg
    32461 ttgctccata acctgccgcc agttgttcag tggtcacaac gcgttgaccg cgatattcga
    32521 taatttgcaa atcacgggct gccaccggca caagttcagt tgattttttc atcattacac
    32581 ctcacgaaaa gctgattagt ggaacactct ggtttccggt tctgagttgt cagtggcttc
    32641 actgataagc ccatccagaa tcatgtgcat cgctctgtaa ccttccattg tgatgccggt
    32701 tttttcgccg tcgcccagag cgccggtgaa tgctttgtac ataagaatgc ttttgagcag
    32761 ccctgattcc gctccgtaag tttctatgag tgcccattcc atatagttct gcatagcgag
    32821 acgtaatggt ttcaggtaga ttgtcatgcc accgttgtgg cctctgtaca tggtgccctt
    32881 gtctgtaccg ccattctcgt taagcacttc ggtccagccg tttctggtga gctgttcaga
    32941 gaagaacatg gcggtataaa gccagcgcca gtacatgatt gtcttttccg gcgctagctc
    33001 acctgtcatg cctttttctt ctgcttcact gatgcaacac actatttcca gaccatgcca
    33061 gatcggctcg tcaaaacagc cttcatcgag catttccagt gctttgttga tatcaagcag
    33121 tccgtaaggt gtgtggatac cttcagcggt aagaaaaaat aactgttcag ttgcagtcat
    33181 ggtttgctct cttcttaaat ggtctttgcg cgacaagccg gcaatactga tcaatccagg
    33241 cgttgtattt ttccatccag gcatcaaact gttttctgcg tgcaaggatg cgcaacaggc
    33301 gatgaaaaca acgcccgtga gctctgtagt attcgggcgt aatttctccc ggatgaaaga
    33361 cttctccata ttccggatgc atcaggcgct ggtcaggcag acgtttttca aaacctgaac
    33421 tttcgaatac cttcgttgtc ataaaatgag cgagatgtat gatggccgtt tttcgcgtga
    33481 agcatttctt aacttgtccg tggcggcaga ctctgtaaac agggccgcaa tcgagttcgt
    33541 atttatgaaa agcacggtcg attccggcat tatttattgc actgtattcc gcatcactga
    33601 agaaaggtgg aaatacgatg tatccttcca ttgtcagcgc cagaacgcgc ggatcggttt
    33661 ctgtcagcca ttcgttttct gtgaattctt cagaattaat ttcaatgatt tcaggttctt
    33721 ctttcatggt tactccgtgt tcatccggtg ttaattactt ttattcactc tgagcgcagc
    33781 cagaagatgt ggctcagcaa ttcgtttgtt gcgatatatc tgtgatacag cctggccttt
    33841 tcaggtcgta aaaatccccg acaataaagc tgtaaattaa ttactgttct taaataatgt
    33901 tattcgtttt cagtccggca cagatgcttt tctgctttgt ctgctgtaca ggataatgag
    33961 cggaaaagac aattcatcat gtttagcgca tctggtgaac caatatcacc gctgtcaata
    34021 atcacctgta acaaagacag gctttctgtt atctcgcagt ggacattatc gagtacttca
    34081 acgcaattat tcatttttca ttatctccgt tattccgggg gaggagctat gctgttaatg
    34141 attaacgcat ccggtaatat gcccctcttt aatgtaatcg atgcattcat tcaaaagttc
    34201 atcaatgatt aactttcctg attcagtcag atactcacca tgttggttga taccgacagc
    34261 atcctggtat gcagtgcgta ttgctgtttc accatctttc cgcccaaact caccacgggt
    34321 aacgccttca aatcgtaata gcaactggct tatgaactgt tctgttattt caatagttgt
    34381 taactctcca tcatgacgct ttacagtgag gagattactt cctgtttttc gtttcatccg
    34441 ccgcagtgct gcaaccgtta tgcggcgacg atatgcagaa atgtattcat ctttcatttg
    34501 ttatttcccg tatgcctttt tgagaaacaa tactgcaact gaccagtatc cggcatcagc
    34561 cattaataaa gcggttttat aggcatgttt gtttttcatg tgtcatcccc attttaaaat
    34621 agtgagaact cccgaccgca gaccgtcatg tttttatgta tgattgtctg tttaatttcc
    34681 tttattcgtt ctcagagcat caacatactc gtaagccttt tcacaggttt tattcatgga
    34741 acgaatcaga caaagtaaga aatcatctgt ctctccgtta tcaccagagt ttttaaatat
    34801 aaactcaagc attgacgtgt tttcttttat ttctgctgcc acttcctcaa gcataatcag
    34861 gggagttttc atgttctttg ttccttaaat gcatcgcatg cgcttctggc gtattgttgt
    34921 gccagtaaaa atatgtcatc cgaaagttca tcacattctt catcaccgga agccgaaatg
    34981 attaaccccg cttcaagcag tactgcaatg tgatgaaaag ctgtttccgg ttcgttggtg
    35041 aggcctttga acattttcat cttacgattc ctttgtcgtg tcagaatgga aacttgtaag
    35101 taaccacatc ggatcgcagt caagtatatt tgccagagga atcacctggc ttgccagagg
    35161 ttcagccttg ccgttctccc actggatgat ggtttcttca tcaactccaa gcagcgtggc
    35221 gagttcagcg gtggatagac cgcagctttc gcgttgggtg cggatgccat cacggcaatc
    35281 ttttttgttt ctcggaggta acagaatatg ttttagttgt tggatttttt cgattccaac
    35341 ctcaaggagt acgtgacggt ttctttccga tacagactcc agactttcat gaagcagcga
    35401 ttctacttgt tcgataagca tcaattgtga agtagacata aaaaaacctc gcaaccaata
    35461 tttcgaaatc taggttacga gtgagtaatg taggtgtcaa gagcaattaa tgttttttgt
    35521 tgaattaaat ttgcatacgc atctcaacga caacaccaat aatttcacaa tccccattga
    35581 tggggatcag tggatagcgt gggtttagtg gctttaggaa acattgccca gcgtcttcta
    35641 tgtactgttt aagggttgcc tctccggagt ttttgagcct ggcgacgaca tattttcctg
    35701 aaaaaacttc tttgtcaggg ttaacaagaa tgttcatgcc ttcaaaaata gagaactctc
    35761 cattctgtga tgtcattgag tcccctttca cttccagcca gaagccgtga tcgcccgcat
    35821 atatatcgct accaatccat tcttctggat aagctatgta atcttcgcag ttccaggggc
    35881 cagcactcac ccatgttagt ttcggatatc tataacggtt tggagaaact ggcttaggat
    35941 ttcggacgtt ttgaatgcta tctgtaggtg atggggcgct gccgtcaaga agccatttag
    36001 gagagcattt caatgcatcc gcgattctaa ataggtttga agatcttgtg tcttgtgagt
    36061 ctcctaactc tattttgctg attgttacac gcgagacacc agtcaatttt gccaggtcat
    36121 cttgtgacat tcctagctgt actctgcgca ttaacagtct gccaccaaag ctatcgtgat
    36181 ccatcttctc ccccttcttg tcgaaataat cgtttcgaaa caaatatagc aaaaaatctt
    36241 gaaatctagg tttcggaaaa ttaagattaa tcgaaacaaa cgtgtcgagg tggatatgga
    36301 tttgtatgaa attctaaaaa acatgtttgg atcgaatgtt gaaataggtc gttattttcc
    36361 ccgaagggga agggctagaa ccggacaggc tgtaggtaag tggaaaaccc gaggtgtgcc
    36421 ggaagacgtg gcgatccttt gtcatctgga tcccaaaatt ccttatcagc atccgtcgct
    36481 catgaatgcc agtcatgaat cgtgatgttc tgccggaggc tgtatgtccc ctgattacgt
    36541 tcagatggag atgcctgcca ggtacagcca ggcagattca gagtggatta agcggcagtt
    36601 acagagcctg ccttcctcac tgagacagaa ggttgccctg aaatacgcgg aggtttacga
    36661 agttactttt gattccgagc ctgtttcatt ccgtaaggag aacagagcga ggcatgaagc
    36721 caatgtacgg ctgcgcaggt tcgttgaaac acacgggcgt gcattacagg ggtatacgac
    36781 tcagccgccc ctggcaggaa cgcaacagcg tacctgaatg gtgttggtct taaaggtgac
    36841 cgacgggctg agaccccact tctcaggatg cacctgtgta ctaccaagct agtgcataag
    36901 ttgagagggg gggtaagggg gggagtgccc gtgtgttagt gcgaagcact ggaacgggat
    36961 cttccaacag acgggtacag aggttaggta gatctcgatc taaaggggca tccctgaaaa
    37021 aacggacgta tcagcaaact agtacatcat ggttaaaata tgagagctga actgaaacag
    37081 gtactaatca accttctgga agaggaattc ggacatggaa tggacaaggt cacattcgac
    37141 tacgttctgc aaaagaaaat caaatctctg ggctgtgagt tgcaacgctg tttcactgtc
    37201 aggctgaaag gtgatcgcag gggatttatc gatcttctgg ttatttctcc cgatggtcag
    37261 cgttgcgcag ttgaggtgga taaccgatcg ccccgtcagc gttcactgat gaaaattcga
    37321 gcgcttcctg aaggcatttc gggatttgtt tttctccgtg atggcaaaca tccacagcgt
    37381 tatatcgccg atggtgtgga tgtcatccgg gcgacccggt ttaagtaatc actgtatcag
    37441 ccctgtcatc gggagaatac catgctgaat atcaagccga attttgctca ggaacgtgcg
    37501 ctgaatatgt tgcgtcatga ctggaaaaca tatcagtcct tcatgatgta catgcctaca
    37561 gggagtggta aaaccggcct ggcggcgttc atcactgctg gtctggtaag ccgtggcatg
    37621 cgcgttctgt ttgtcgttcc ttataccatt ttgatcaacc agacagcaca gcgctttgtt
    37681 cagtacgggc tttccgggga tgaaatcagc tttatctggc gtgatcatcc caataacgac
    37741 ccgtcacgac tgattcagat tgccagtgcg gatacgctga ttcgccgtga ttttcctcag
    37801 aatatcgacc tgctgattat tgatgaagct catctccgta agcgccgcat cctcacggaa
    37861 atcgaacgac tggttgcaga gggaaatgtg aaggtgattg ggttgtccgg tacacccttt
    37921 tcatcgttcc tggggcgtta ttaccagcgt cttctcaagc cgaccactat aggggaactg
    37981 atccagcgtg gcgaactgag caattacgaa ttttatgcgc ccacgaaacc ggatctggaa
    38041 ggtgtaaaaa cgaaagtctc aatggagtac ggcagggatt acgacgaatc acaactggca
    38101 gagattatgt gcggcgcgga tctggttggc gatattgtcg ataactggct gtgtaacgga
    38161 agggatttac cgacagtggc gttctgcgtc aataaaacgc atgccagctt cgtgaccatg
    38221 cagttcaaca aagccggaat taacgcagag gtgatggttg ctgaaacgcc acatgatgag
    38281 cgtcagttaa tgatcagtcg ttttgaaacg ggagcgacga aaatcattgt cagcgttggc
    38341 gttctggtgg cgggatttga cagtgatgtc cggtgcatca tttacgcacg cccgacaaaa
    38401 tcagagattc gctggcttca ggcgattggg agaggattgc gtacagcgcc aggcaaggag
    38461 tcatgcctga ttttcgacca ttccggtacg gttcatcgcc tgggatttcc tgaaaatatt
    38521 gagtatgacg aactgcccgg caaaaacgac gggatgcagg tgtcatcatc acgtggtaac
    38581 gaaattcggg aagaaaaact gccgaaagaa tgtccggggt gtcatttcat gaaaccggca
    38641 ggtgtttatg tctgtccgaa atgcggattt aaaccgctgg ctggtgagga tgtgaacaca
    38701 gatacccgcc gcaatatcag gaaactcagc aaggacgaaa aaatttacac caaaagcgac
    38761 aaacaggcct ggtggagtca gatcaaattc tatcagcgtc agcgtgcctc tctggggcga
    38821 ccggtcagcg atggatggtg cgctcacact ttccgggaga agttcggcga atggcctgac
    38881 gggctgagca gttttccgat ggaaatcacc ccggaggtca gtaattacat cagacacaaa
    38941 ttcatccggt ttgccagagg gcgtagccgg gcagagaaga tgacggaaaa atcccccgaa
    39001 gcgctttctc tgtccctgct tcatggtgtc cggtctgggg tgccggaagg cagtgagtcg
    39061 tggcagatca tgcaggcaaa gcaacaactt cagaaaaaca gaaacagtct gagtcagtaa
    39121 gatgaaaaca gcagaagcag cgaaaggcca ctggcctgaa attttaaaac attacggact
    39181 gccgccagtc acgggtaaaa agcacttcaa aggggaatgt ccggtttgtg gtgccagagg
    39241 taagttccgt attgatgacc gtgatggcac cggaacgtgg atctgtgttt gtggtagcgg
    39301 tgacggcatg aaactgatcg ctctgacaca aaaaagagcg tttcatgaaa tttgcgctga
    39361 aatagaccgc atcacgggga atgaataccg gagagataaa ccgcctgtga tctgtacgtc
    39421 ggaaagcctg agatcgcgca ttcagcgtcg attttcagcg ctcacgtcgc tacagggaac
    39481 atctggtgct gaatatcttc gttcccgtgg catattcagt cttccggttg agggagttcg
    39541 cttcaacagc cagcaaaatt ataacgggcg catgttccag tctctttatg ccctggcgac
    39601 cgacgacaag ggagaactgt gttacctgca tcagacattg cttgatggag ctaaaaaagc
    39661 agacattggt atcagcgcca gaaggctcaa atctttacag gacgataact atctggacca
    39721 tgcccggtca gttgctatca ggatgtttcc ggtagccagt acactgggta tagctgaagg
    39781 tatcgaaacc gcgttgtccg cacatcagat ttacaaagta aatacctggg caaccattaa
    39841 cagtggtttt atgaaaaagt tccgcgtacc ggcgggagtc acccatctga ttatttttgc
    39901 cgatcgcgat gaatacagtg ctaccggaat ggctgcggcc tgtgaatgcg cacatgcaaa
    39961 tctccaggcc cgaaatgaca ttcagcgggt aagtgtccgg tggcctgatc atgatgattt
    40021 caacaatatg ctcatgaacg gcgatcaggt tcgggaactg gttttttaca gaaaaaaggc
    40081 ggttgcgtga tgcgtacgga caatcaggta cataaagctt tattcactat cccgacggca
    40141 gcgtacagcg ccgttccggc aaatatcaaa cctctgccag aacaaaggag gatcaccgga
    40201 cataaacaga cagatgctta tctctggatt ctggaggtta ttcacctgaa cgaggccgta
    40261 catctggacg cagccgaagc agcactggag aaactcaaaa taacgccgga agaagcgagt
    40321 gaacggtatg gacgttattt gcaggagatt aatgttgatc ctttccagat tgctttcgca
    40381 accataggca tagataaccc ggcgcaggcg atcaggaatg cccgtgagaa tatcaaaaaa
    40441 gccgcatcag tcagggccac atttggcagc tatgaggcag cactcgatga tgtggaggct
    40501 gagcggataa ttcgcacctc cccgaaattt atcgacgatt actactgggg ctggactgcg
    40561 gcagagaaaa aagccggaag tattgacggt gttcgctcaa atgaaattga tgatcagcgt
    40621 cgtgcatatg ttgatggcta tcgtgatgta ctgccagagc ctcatacatt gtcagacgtt
    40681 gttcgtgagt ttatttactg ggactggctt tatgagatgc gccagacagc ggggagagaa
    40741 acaggggaca aatacggttt cacaggtgaa catcatgagt cagtatatga tcggcagttc
    40801 tggctcgaaa atttgctggg aaaaataaag cctgtgacgc gtgatgaagc ggttgaagtg
    40861 tgtcgctggt ttctggcaag cggaaaagat gagtacatgg aagacaatgg ttcagcggtc
    40921 attctcaatc tggttgggga gtgtgagcaa tgaagccgga gatatccagt agacattttc
    40981 cccctgagca ttcaaatcag aaaaagtatg gttacagcgg agtacagaat aatgcgtgat
    41041 atccaacagg ttctgtcccg atggggtgca tgggtggtaa ataatcatga gagtgtggca
    41101 tggtccggcg tcgctgccgg atttaaggga gtgatcccat caaaggttaa atcacggcct
    41161 cagtgtaccg atgatgatgc gctgattatc agcaattgca tggcgcaact gaacgtcaac
    41221 aacagcgatt tgcatgattt tctgtatgat tattatgtgt tcgggatgac gcttatggga
    41281 ctgggccgta agcatgagcg ctctgattgc tgggccgggc gggtactgca aaaagctgaa
    41341 ggtgttattg aggggatgtt gattatgcag ggaataaaac tggaaatgga cagatacgtt
    41401 gagcgtgaac gatcaggggc acaggtcagt cagttttccg gacatacggg aaattgaaag
    41461 cgcggggttt tactgtagaa tgactgcggg tgcttgaggg tgtctgcctc gggcatgctg
    41521 ccgtaaggca gacggagaaa agccccagtt aacattacgc gtcctgcaag acgcttaaca
    41581 ttaatctgag gccaatttca tgctgaacac atgtaggtta gcctcttacg tgccgaaagg
    41641 caaggagaag caggctatga agcagcaaaa ggcgatgtta atcgccctga tcgtcatctg
    41701 tttaaccgtc attctgacgg tactggtagc gaggaaagac ctctgcgatg tacgaatccg
    41761 aaccggccag acggaggtcg ctgtcttcgt agactacgaa tctgagaagt aagagacctg
    41821 gcgggggagt aatctcccgc cccctctgat gtgtcaggca tcctcaacgc acccgtactt
    41881 tagttgtcat atgctccgca aaatgcaggt tgtcgttgca acccgtgcgg taattttttg
    41941 tgtgcgagtg ctcaaaaaac gttgattttc ataaaaggac gattttatgc tgccagacca
    42001 gcgaccagcg accgtaaatc aagaaagtct gtaaaatctg ttgaaccaga cattgccgac
    42061 cgggctaaca actggctcaa atcttatgga cttgattaca aactggagca ggcaccactt
    42121 aacaacgaga tcgataacgc actggatgct tatttttcga aaaatggcgg cacaggcggt
    42181 aatcgtcccg acgtaaaact ccttgttcag gacaaatacg gcaagcagta tcccgttctc
    42241 atcgagtata aaggctacaa ggacaggctt atcagacttg gcagcaacgg tattattgag
    42301 aacaaagatg ccagaaaaga atggaatttt aaaaatatta atggatacgc ggtgaatggc
    42361 gcagtccatt atgccaacgc acttcttcag ttcaccaatt accctgatat tattgctatt
    42421 ggtatgaccg gctggcgcga tgatggtaca ggcgaactgc atcatgaaat cggcgtgtgg
    42481 tacgtatcaa agaataattt aggagccggg cagaaggttg gtgaattcac cgacttatcg
    42541 ttccttgctg acaaaaatgt tgatggattt ctcaacaaga taaagctgct taatcttcct
    42601 cccgaagaac tggaaaaaat caaggccagc aaagaggaag agatagacac gcgactttcc
    42661 cgtctgaata acgatattta ccagaatgaa aaggggcttg gtgaaagcga tcgtgtttac
    42721 cttgttgtgg ctaccgttat tgcaacgttg gggattccgg gaaagttagc accgctggat
    42781 aaaaaagaac tgacctcttc aacggaggag gacttgcgtg atggtgatat tattttcagg
    42841 aaaataagga attttttaag gctgaaagct gttcctgaaa caaaaagaga aatgattttg
    42901 cgctcgttgc aaaatacgct gtggactgaa aacattaaca aaccagtcaa cggtgaaagc
    42961 cagttaaagc gcgtgtttgt taaagttgtt gatgatcttg gggagtatta caaaatcgga
    43021 ctgaccactg attttacggg caaactgttt aatgagatgt atcgctggct tggatttacg
    43081 caggacaaac tcaatgacgt cgttctgaca ccaccttatg ttgccacatt gctggcacga
    43141 ctcgccagag taaacaaaga ttcctatgtg tgggattttg ctacaggttc tgcgggattg
    43201 cttgtggctg caatgaatga aatgctcatt gacgccagag aaaatattca ctcaccgaat
    43261 gaactacaac ttaaggaagc gcaaatcaag gctgaacagc ttcttggcct tgaggtgtta
    43321 tccagtattt atatgctggc catcctgaat atgattttga tgggggatgg tagctcgaat
    43381 atcctcaata aagactcgct agccgatttt gacggcaagt atggatttgg gaaaacagga
    43441 gaaaaattcc ctgcggacgc atttattctc aatcctccgt attcagctaa aggtaacggc
    43501 atgattttcg tgcagaaggc gctgtcgatg atggataaag gctatgctgc agtgattatc
    43561 cagagttcag ccggcacagg aaaggctacg gagtacaaca aaaaaatact caaggaaaat
    43621 accttgctgg caagcatcaa aatgcctgcg gatctgttta ttggtaaatc aagcgttcag
    43681 acgtacattt acgtttttca ggtaaaaatt ccgcataacg cgaaacaggc tgttaagttt
    43741 atcgactttt ccaatgacgg ctatgctcgc tctaatcgca aaaaggcaag aaacaatctt
    43801 gttgatgccg atcgtgcaaa agagcgttat caggaggttg tggatctggt tcacttcggg
    43861 aaaggttgcc ttaatatctt tacggaggat gaatattttg agggaacaat cgatcctgat
    43921 agtggtgagg actggaatca gaccaggcca gtagatgcta ggccaacgct ggatgatttc
    43981 aaaaagacgg taggtgatta ccttgcatgg gaggtctcgc aattactgaa aaaacaaggt
    44041 gaaaacaatt ttgcgggaaa gtaaattccc cgctcagcga taaattaaga agcgttgagt
    44101 ggggagagtt caggattggc gatttgttct atgtgagcac aggctcgctt atctcctcag
    44161 gaagattaaa accaggcaaa attaaacgtg tttcagcaaa gagcgatcaa aacggtatca
    44221 tcggagagtt tgataccgag tttatggatg atgcaaggca ttatgaaaat tttataagcg
    44281 ttaatttttt tggagatgct ttttatcatc cttattgtgc cagcgtagaa atgaaggtgc
    44341 atgttcttac attaaaatca agtaagtccg actttaatgc aaagcgcgga ctgtatgttg
    44401 ccagtatgat taataaagct ttaaaggggc gttttaatta tggtcaacag ttatcgagtt
    44461 ccaaactcag agatggagaa tatttcatct cattaccagt gaaaaataat gaaattgact
    44521 ggttttttat ggataattat atagaagagc tggaagcggc ccatatagaa gagctggaag
    44581 cggcccatat agaagagctg gaagcttact tgctggtt
//
LOCUS       DAZKHK010000023        44488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKHK010000023 DAZKHK010000000
VERSION     DAZKHK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.023"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..1062
                     /gene="cysB"
                     /locus_tag="ACHOS5_001312"
     CDS             88..1062
                     /gene="cysB"
                     /locus_tag="ACHOS5_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP9776153.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1381..1521
                     /gene="ymiA"
                     /locus_tag="ACHOS5_001313"
     CDS             1381..1521
                     /gene="ymiA"
                     /locus_tag="ACHOS5_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP9776154.1"
                     /translation="MRLAMPSGNQEPRRNPELKRKAWLAVFLGSALFWVVVALLIWKL
                     WG"
     gene            1524..1691
                     /gene="yciX"
                     /locus_tag="ACHOS5_001314"
     CDS             1524..1691
                     /gene="yciX"
                     /locus_tag="ACHOS5_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP9776155.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1805..1900
                     /gene="ymiC"
                     /locus_tag="ACHOS5_001315"
     CDS             1805..1900
                     /gene="ymiC"
                     /locus_tag="ACHOS5_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP9776156.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2051..4726
                     /gene="acnA"
                     /locus_tag="ACHOS5_001316"
     CDS             2051..4726
                     /gene="acnA"
                     /locus_tag="ACHOS5_001316"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP9776157.1"
                     /translation="MSSTLREASKDTLQAKDKTYHCYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEVSLAGPKRPQDRVALPDVPKA
                     FAASSELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTC
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4790..5380)
                     /gene="ribA"
                     /locus_tag="ACHOS5_001317"
     CDS             complement(4790..5380)
                     /gene="ribA"
                     /locus_tag="ACHOS5_001317"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP9776158.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5550..6314
                     /gene="pgpB"
                     /locus_tag="ACHOS5_001318"
     CDS             5550..6314
                     /gene="pgpB"
                     /locus_tag="ACHOS5_001318"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP9776159.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6463..6771
                     /gene="lapA"
                     /locus_tag="ACHOS5_001319"
     CDS             6463..6771
                     /gene="lapA"
                     /locus_tag="ACHOS5_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP9776160.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            6778..7947
                     /gene="lapB"
                     /locus_tag="ACHOS5_001320"
     CDS             6778..7947
                     /gene="lapB"
                     /locus_tag="ACHOS5_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP9776161.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8138..8875
                     /gene="pyrF"
                     /locus_tag="ACHOS5_001321"
     CDS             8138..8875
                     /gene="pyrF"
                     /locus_tag="ACHOS5_001321"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9776162.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8875..9201
                     /gene="yciH"
                     /locus_tag="ACHOS5_001322"
     CDS             8875..9201
                     /gene="yciH"
                     /locus_tag="ACHOS5_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP9776163.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(9327..9545)
                     /gene="osmB"
                     /locus_tag="ACHOS5_001323"
     CDS             complement(9327..9545)
                     /gene="osmB"
                     /locus_tag="ACHOS5_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP9776164.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9814..10563)
                     /gene="yciT"
                     /locus_tag="ACHOS5_001324"
     CDS             complement(9814..10563)
                     /gene="yciT"
                     /locus_tag="ACHOS5_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP9776165.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNATIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10653..10826)
                     /gene="yciZ"
                     /locus_tag="ACHOS5_001325"
     CDS             complement(10653..10826)
                     /gene="yciZ"
                     /locus_tag="ACHOS5_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP9776166.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            10864..10992
                     /gene="ymiD"
                     /locus_tag="ACHOS5_001326"
     CDS             10864..10992
                     /gene="ymiD"
                     /locus_tag="ACHOS5_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP9776167.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            complement(10974..12959)
                     /gene="pdeR"
                     /locus_tag="ACHOS5_001327"
     CDS             complement(10974..12959)
                     /gene="pdeR"
                     /locus_tag="ACHOS5_001327"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9776168.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSMFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            13013..13116
                     /locus_tag="ACHOS5_001328"
                     /pseudo
     CDS             13013..13116
                     /locus_tag="ACHOS5_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13193..15127)
                     /gene="rnb"
                     /locus_tag="ACHOS5_001329"
     CDS             complement(13193..15127)
                     /gene="rnb"
                     /locus_tag="ACHOS5_001329"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP9776169.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(15195..16322)
                     /gene="yciW"
                     /locus_tag="ACHOS5_001330"
     CDS             complement(15195..16322)
                     /gene="yciW"
                     /locus_tag="ACHOS5_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP9776170.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(16467..17255)
                     /gene="fabI"
                     /locus_tag="ACHOS5_001331"
     CDS             complement(16467..17255)
                     /gene="fabI"
                     /locus_tag="ACHOS5_001331"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9776171.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            17733..18299
                     /locus_tag="ACHOS5_001332"
     CDS             17733..18299
                     /locus_tag="ACHOS5_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9776172.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            18448..19569
                     /locus_tag="ACHOS5_001333"
     CDS             18448..19569
                     /locus_tag="ACHOS5_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9776173.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            19569..22676
                     /locus_tag="ACHOS5_001334"
     CDS             19569..22676
                     /locus_tag="ACHOS5_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFP9776174.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            22680..24053
                     /locus_tag="ACHOS5_001335"
     CDS             22680..24053
                     /locus_tag="ACHOS5_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFP9776175.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            24062..25225
                     /locus_tag="ACHOS5_001336"
     CDS             24062..25225
                     /locus_tag="ACHOS5_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFP9776176.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRGVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWVQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(25273..26079)
                     /gene="sapF"
                     /locus_tag="ACHOS5_001337"
     CDS             complement(25273..26079)
                     /gene="sapF"
                     /locus_tag="ACHOS5_001337"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP9776177.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(26081..27073)
                     /gene="sapD"
                     /locus_tag="ACHOS5_001338"
     CDS             complement(26081..27073)
                     /gene="sapD"
                     /locus_tag="ACHOS5_001338"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP9776178.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(27073..27963)
                     /gene="sapC"
                     /locus_tag="ACHOS5_001339"
     CDS             complement(27073..27963)
                     /gene="sapC"
                     /locus_tag="ACHOS5_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP9776179.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            28098..29327
                     /locus_tag="ACHOS5_001340"
     CDS             28098..29327
                     /locus_tag="ACHOS5_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9776180.1"
                     /translation="MCTLKCIRNTRVYTLLTDTKLRKSLGKKRDKIEVLSDAQGLNVR
                     LSTSGSITFFYRYRWNGKAAQLAIGSYPSISLAQARERRQQFRDWLTEGLDPRRQVML
                     EKQKKSQAMTVKEAFDYWEQHYCIPEGLKKLKVNRRDFNNHIAPVLGNVIVEQTTKAH
                     WLSLFDSMGRRVVTGQMLGLMQRTFRFCSNRGVISINPLESLRRSDVGMAAATRDRKL
                     SDEEIKIVWSALSDMGERQKLVVKFLLMTGCRTAEIRTARWEWFDFNENTWTVPASEY
                     KTGKTVRRALPPAIVKLMQEVKKTSVSQHVLTRARYNGPEDDKPPQQPHIALFSAQII
                     AKTGMKTWSLHDLRRTVATRLSELGAPPHVVEKLLGHHMSGVMARYNLHDYLEDQRHW
                     LQVWQNYLEGLVDAPLT"
     gene            complement(29291..29533)
                     /locus_tag="ACHOS5_001341"
     CDS             complement(29291..29533)
                     /locus_tag="ACHOS5_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9776181.1"
                     /translation="MNDITKTNEILLTKDILERYKISRSTLYFWSTPDRKPKSLSKPF
                     PKPTIKGNPNRWWLSDILEWERNNAQVNGASTNPSR"
     gene            complement(29587..29838)
                     /locus_tag="ACHOS5_001342"
     CDS             complement(29587..29838)
                     /locus_tag="ACHOS5_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9776182.1"
                     /translation="MFVLIQRGQSFVDANNYPVEVCKITLTQVIFRRLDGRTRAASIS
                     AFNAEFERIDHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            complement(29887..30567)
                     /locus_tag="ACHOS5_001343"
     CDS             complement(29887..30567)
                     /locus_tag="ACHOS5_001343"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9776183.1"
                     /translation="MTSDIILQRTGIDVRAIVQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFESIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(30564..31349)
                     /gene="bet"
                     /locus_tag="ACHOS5_001344"
     CDS             complement(30564..31349)
                     /gene="bet"
                     /locus_tag="ACHOS5_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9776184.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGKEITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAHTAEHQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRALSELKQDEAVKALGFLKQKAAEQKVAI"
     gene            complement(31355..31651)
                     /locus_tag="ACHOS5_001345"
     CDS             complement(31355..31651)
                     /locus_tag="ACHOS5_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9776185.1"
                     /translation="MNAWLIPDRIEEQSWARHYQQIAREETEAELADDLEKGLPQHLF
                     ESLCIDNLQRHGASKKAISRAFDDDVDFQERMAEHIRYMAETIARHQINIDSEV"
     gene            complement(31648..33720)
                     /locus_tag="ACHOS5_001346"
     CDS             complement(31648..33720)
                     /locus_tag="ACHOS5_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFP9776186.1"
                     /translation="MSADKQTFALHCEAKNDKVRKRLGIKGGFFWTEARKLSVAVSRC
                     IAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTEFCNRYEKGGNDGITMMAIPFD
                     DNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
                     LFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRAAHHELDKLTNHHLSR
                     LAESVGKAFPHSANHRISPAEFDKFISTWMKTDYLDQGLLTKEWQNGNYVSGITRTPS
                     GANAGGGNLTDRGEGFKHDKTSLARDVATGVLARSMDVDIYNLHPAHAKRVEEIVSEN
                     KPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHTN
                     PDPEIVEIACGRSSAPMPQRGTTEEKHNDEEKQQASDTMANEQAAPESVEKIPVKHNE
                     DTQSLENVSSVETKYQKLREELNKARENIPPRNPVDADKLLAASRGEFVEGISDPTDP
                     KWIKGIQARDTEDQNQSKVEQIAPEAGQNSPDTQQNGPEEQQPAPVAQPELEKNCRVC
                     GQTGGGNCPDCSAVMGDSTYTETFGENDAADGEDSAQTEEKIIQENSVDAAQEGETVV
                     QNEPGSDTSGDDANSEPVTLDWKRQLVIAAVYGLCANPACIATAPAIPDIAIMIANRL
                     ENFGGDKS"
     gene            complement(33828..34214)
                     /locus_tag="ACHOS5_001347"
     CDS             complement(33828..34214)
                     /locus_tag="ACHOS5_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776187.1"
                     /translation="MKELAQNEIFSETSPDAINELKEIAERISKICKEYQIDFVFSFS
                     VLTEVGNNEYKDSRFVLCGLNGKTPSPYIHAAREVVRSNIGAQQIHTLAQALEFAREN
                     SECDCPECQHEKEKTTHKTANQATFH"
     gene            complement(34300..34521)
                     /gene="kil"
                     /locus_tag="ACHOS5_001348"
     CDS             complement(34300..34521)
                     /gene="kil"
                     /locus_tag="ACHOS5_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560216.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9776188.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIEKRKLYIR
                     SLTTKTCITNCMIKVFIGRDGLPVKAESL"
     gene            34980..35189
                     /locus_tag="ACHOS5_001349"
     CDS             34980..35189
                     /locus_tag="ACHOS5_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776189.1"
                     /translation="MTQEEKVMFLMRLAVDTYNTQFREKDIPQKAVPAAVDKGGAIAV
                     FYDAFESFFDEKLDAVSDFGTSSNK"
     gene            complement(35158..35517)
                     /locus_tag="ACHOS5_001350"
     CDS             complement(35158..35517)
                     /locus_tag="ACHOS5_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776190.1"
                     /translation="MNETELKHIIAMLLEDARQVYRLSPNSATQERIRMAETALKQDN
                     GNGISKFSAASENEVIEINSNISDSCVAYSHQIIRVSARIMEVMASELEKNNIKPTDC
                     CLRTVMNVIYYSMFRSR"
     gene            complement(35549..36262)
                     /locus_tag="ACHOS5_001351"
     CDS             complement(35549..36262)
                     /locus_tag="ACHOS5_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776191.1"
                     /translation="MTTPSVLPQKLWRPLAEIKNFVEKMPDGVRLTEVTKKVKTFAEL
                     SGKERNQLIDFIDKRESIIVFKVRKEGSGNGVTFFRHKKYGYPKREGNVTIIKDLQSK
                     LCTKCGQTKSVNDFYSDASKRDGRAIYCKKCESAMKRSRRECNKLILQQQEPEMNNLK
                     AVSPSPETLRKQAEELLKAAEIAEKKRQEDDVFNKKLAPLKLEILQAAGKMQLKLDEF
                     IDCMDEMNKAVQKLKELAA"
     gene            complement(36266..36538)
                     /locus_tag="ACHOS5_001352"
     CDS             complement(36266..36538)
                     /locus_tag="ACHOS5_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP9776192.1"
                     /translation="MSRKTEFKGTAASRRRARRAELQGLEASSADKLHRPTPSRVYLQ
                     CKRKAAMRAEVVTITTLTRKYEGSTCLPNTALYAAGYRKSGTITAR"
     gene            complement(36516..36690)
                     /locus_tag="ACHOS5_001353"
                     /pseudo
     CDS             complement(36516..36690)
                     /locus_tag="ACHOS5_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36935..37705)
                     /locus_tag="ACHOS5_001354"
     CDS             complement(36935..37705)
                     /locus_tag="ACHOS5_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9776193.1"
                     /translation="MVKKDELKEAFSKRLLQALLDAGVGGRGQAKRIQNAMKLRGIDI
                     SEPGIWKWLNSASIPEKTSILALSDWLSVRPEWLEYGSNEPSVKQRPSIPNESEWGSL
                     ETWDRNTPLRDDEVEVPYLKDIEFACGDGRVHCEDHNGFKLRFSKATLRRVGANSDGS
                     GVLCFPATGDSMEPVIPDGTTIAVDTNNKRIVDGKLYAIAQPGVGDEKLKRIKLLYRR
                     PGGKLIIRSYNSEEYPDEEADTQSVEIIGKVFWYSVLL"
     gene            37790..38017
                     /locus_tag="ACHOS5_001355"
     CDS             37790..38017
                     /locus_tag="ACHOS5_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776194.1"
                     /translation="MNPIQFAVKAVGGQTVAARLCGLSNVAIHKWVKNGALPRTEYTM
                     KTNYSEILANASDGKFTAEWLRNVANPDRQR"
     gene            38161..38457
                     /locus_tag="ACHOS5_001356"
     CDS             38161..38457
                     /locus_tag="ACHOS5_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9776195.1"
                     /translation="MSQTSYSKLSQRDIDRAETDLLINLSALTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAINVHANKKRPVGAGRSEQILMNI"
     gene            38472..38690
                     /locus_tag="ACHOS5_001357"
     CDS             38472..38690
                     /locus_tag="ACHOS5_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776196.1"
                     /translation="MEQYTVIILPHLEEFRQQQHQPQQPEADWVNPEIPGPSVKMCSH
                     TNVQSHQPQQPEADWVNPGDTGTVCGME"
     gene            38711..39793
                     /locus_tag="ACHOS5_001358"
     CDS             38711..39793
                     /locus_tag="ACHOS5_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9776197.1"
                     /translation="MRNYATISPQFWLGDTGRKLRKSGPECMVVALYMMTSPHSNMLG
                     LYYLPVLYIAHETGLDPEGASKGLQMACEAGFCSYDHDSEVVWVHEMAAWQVGEALKP
                     GDNRCAGVRNEYSALLENPFLSSFYDRYKDDFHLDVRRESCRKIEAPSEPLSSQEQEQ
                     EQEQERDKTLLVHGEKIATDPLVDSCPVLTERPGPAGMTPEADSGRCVQQVLLVEPEQ
                     QHQPQQPEADSAMSGKPIGLTRAMPGPSAGRVDYPDVFERVWREYPHRAGSNPKKSAF
                     NAWRARLREGVSPDVVLDGVRRYARYLEATGKAGTEFVQQASTFFGPNRNFENPWSLP
                     KAGAVSQRCVNHISEPDTEIPPGFRG"
     gene            39800..40540
                     /locus_tag="ACHOS5_001359"
     CDS             39800..40540
                     /locus_tag="ACHOS5_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9776198.1"
                     /translation="MKNISTGGILERVRRLAPPHVAAPFRTTDEWREWQLAEGRKRSE
                     EINRQNHQARVEKILNRAGIQPLHRKCSFENYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVILNQIVDRRTASLRTVGILTNLNHAAMSTLLGERV
                     MDRMTMNGGRWVSFNWESWRSNVGRQGM"
     gene            40566..41336
                     /locus_tag="ACHOS5_001360"
     CDS             40566..41336
                     /locus_tag="ACHOS5_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9776199.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQPEAPDVVTEEVAPKVSADMMIEFISQEGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAASVAETDGKA
                     FPQPAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRA
                     KNHVQKWERVCAALRELNKHRDIVRQIADSSRRVASEK"
     gene            41352..41768
                     /locus_tag="ACHOS5_001361"
     CDS             41352..41768
                     /locus_tag="ACHOS5_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9776200.1"
                     /translation="MAKVFTQEEREKIKVQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDLYNSGYGLFPSEQARKDWIKARKKMSKAAVKKKSDPDLVYSLPDGEI
                     RRYDRRLNIICRECRKSEVMQRVLAFYQGKFQEVML"
     gene            41765..42253
                     /locus_tag="ACHOS5_001362"
     CDS             41765..42253
                     /locus_tag="ACHOS5_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9776201.1"
                     /translation="MSTPATILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFPVVVFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLWPHGVLIFKWNETQIPVRQILALTDAKPIISQRTGKNDKTHWIIFVKEAT
                     SG"
     gene            42246..42500
                     /locus_tag="ACHOS5_001363"
     CDS             42246..42500
                     /locus_tag="ACHOS5_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776202.1"
                     /translation="MGKSKCQVYGNKIEPCTALANSLEHDAEYTTRKGLLIYKIWNEN
                     LTRDPDLVMLRSGEFSKSPVRVSFCPFCGESLKTWENRNE"
     gene            42493..42804
                     /locus_tag="ACHOS5_001364"
     CDS             42493..42804
                     /locus_tag="ACHOS5_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776203.1"
                     /translation="MNEIREIPVERDEYGCWTHPEYEKFCDGREYISTEEFSAWMKAN
                     NLQWTICSMDEDYLNPVADDPDISTWEPERPEGEGWFIGSIHDTEDGPVCVWLRNKVM
                     A"
     gene            42922..43107
                     /locus_tag="ACHOS5_001365"
                     /pseudo
     CDS             42922..43107
                     /locus_tag="ACHOS5_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            43109..43465
                     /locus_tag="ACHOS5_001366"
     CDS             43109..43465
                     /locus_tag="ACHOS5_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776204.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHDSVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARMRRATKSRTE"
     gene            43561..43884
                     /locus_tag="ACHOS5_001367"
     CDS             43561..43884
                     /locus_tag="ACHOS5_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776205.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSEMARVEGELFKIALASLV
                     AEPAGKLHEYKPVGYQRLVDELTMLVKQLAWQLRKAKPGCKLPDKAMDYLERNGLISV
                     EDVLR"
BASE COUNT        11075 a        10417 c        11483 g        11513 t
ORIGIN      
        1 acctttttta ttattaaatc gtattagtca cccgcctggt gtgacgaaaa aacgatgttc
       61 tgatggcgtc taagtggatg gtttaacatg aaattacaac aacttcgcta tattgttgag
      121 gtggtcaatc ataacctgaa tgtctcatca acggcggaag gactttacac atcacaaccc
      181 gggatcagta aacaagtcag aatgctggaa gacgagctgg gcattcaaat tttttcccga
      241 agcggcaagc acctgacgca ggtaacgcca gcaggacaag aaataattcg tatcgctcgt
      301 gaagttctgt cgaaagtcga tgccataaaa tcggtcgccg gagagcacac ctggccggat
      361 aaaggctcgt tgtatatcgc caccacgcat actcaggcac gctatgcgtt accaaacgtt
      421 atcaaaggct ttattgagcg ttatcctcgc gtttctttgc atatgcacca gggctcgccg
      481 acacaaattg ctgatgccgt ctctaaaggc aatgccgatt tcgctatcgc cacggaagcg
      541 ctgcatctgt atgaagattt agtgatgcta ccgtgctacc actggaatcg ggctatagta
      601 gtcactccgg atcacccgct ggcaggtaaa aaagccatta ccattgaaga actggcgcaa
      661 tatccgttgg tgacatatac cttcggcttt accggacgtt cagaactgga tactgccttt
      721 aaccgcgcag ggttaacgcc gcgtatcgtc ttcacggcaa cggatgctga cgtcattaaa
      781 acttacgtcc ggttagggct gggggtaggg gtcattgcca gcatggcggt ggatccggtc
      841 gccgatcccg accttgtgcg tgttgatgct cacgacatct tcagccacag tacaaccaaa
      901 attggttttc gccgtagtac ttttttgcgt agttatatgt atgatttcat tcagcgtttt
      961 gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc taatgaagaa
     1021 attgaggtca tgtttaaaga tataaaactg ccggaaaaat aatttcttgc gttattttcg
     1081 gcacctttta tgtagcgaag gtgccggaat atattctcct ttgttactta tttgtatccg
     1141 gtgcacaata tttttatccc tcatatttat agggtaaatt acctgtaaag tagtgtctaa
     1201 ttacacacct cgcaaactcc agttttcaaa tatctatttc aggtcaaaag attgaattat
     1261 tcactggaga cgattcgtaa attcgctgga tttttgacta aagtttcttt aggatttgtc
     1321 tcatcgtatg attaattaca ccaatcgttc aacgtcttgc tgataagtga tggcgattat
     1381 atgaggttag caatgccttc tggaaatcag gaaccccgcc gcaatcctga attaaaacgt
     1441 aaagcctggc tggcggtttt tcttggttct gcacttttct gggtggttgt cgcactgctt
     1501 atttggaaat tgtggggata actatggtcg gtcaggagca actggagtcg tcaccattat
     1561 gtcagcatag tgacaatgaa ccagaagcaa aacgggaatg ttccgtcgtt attccagacg
     1621 actggcaact aacatcgcag caacaagcct ttatagaact gtttgctgaa gatgatcagc
     1681 cgaaacaata attatcatca ttcttattac ccatttttaa tgaattacag ggcttttaat
     1741 acaccgcagc aataacaggt tgagttatct caacacaaag taataaccgt taagggtgta
     1801 gcctatgatc aacacaaata tgaaatattg gtcctggatg ggcgcatttt ctctgtcgat
     1861 gctcttctgg gctgaactcc tctggatcat tactcactga tccctgaccc cgctgtttgc
     1921 tttgcctcaa ggtttctcct cttttatcaa tttgggttgt tatcaaatcg ttacgcgata
     1981 tttgtgttat ctttaatatt caccctgaag agaatcaggg cttcgcaatc cctgtcatta
     2041 aggaggagct atgtcgtcaa ccctacgaga agccagtaag gacacgttgc aggccaaaga
     2101 taaaacttac cactgctaca gcttgccgct tgctgccaaa tcactgggcg atatcacccg
     2161 tctacccaag tcactcaaag ttttgctcga aaacctgctg cgttggcagg atggtaaatc
     2221 ggttaccgaa gaggatatcc acgcgctggc gggatggctg aaaaatgccc atgctgaccg
     2281 tgaaattgcc taccgcccgg caagagtgct gatgcaggac tttaccggcg tacctgccgt
     2341 tgttgatctg gcggcaatgc gcgaagcggt taaacgcctc ggcggcgata ctgcaaaggt
     2401 taacccactc tcaccggtcg acctggtcat tgaccactcg gtgaccgtcg atcgttttgg
     2461 tgatgatgag gcatttgaag aaaacgtacg cctggaaatg gagcgcaacc acgaacgtta
     2521 tgtgttcctg aaatggggaa agcaggcgtt cagtcggttt agcgtcgtgc cgccaggcac
     2581 aggcatttgc catcaggtta acctcgaata tctcggcaaa gcagtgtgga gcgaattgca
     2641 ggacggtgaa tggattgctt atccggatac actcgttggt actgactcgc acaccaccat
     2701 gatcaacggc cttggcgtgc tggggtgggg cgttggtggc atcgaagcag aagccgcaat
     2761 gttaggccag ccggtttcca tgcttatccc ggacgtagtg ggctttaaac ttaccggaaa
     2821 attacgtgaa ggtattaccg ccacagacct ggttctcact gttacccaaa tgttgcgcaa
     2881 acatggcgtg gtggggaaat tcgtcgaatt ttatggtgat gggctggatt cactaccgct
     2941 ggcggatcgc gccaccattg ccaatatgtc gccagaatat ggtgccacct gtggcttctt
     3001 cccaatcgat gctgtcaccc tcgattacat gcgtttaagc gggcgcagtg aagatcaggt
     3061 cgagttggtc gaaaaatatg ccaaagcgca gggcatgtgg cgtaatccgg gcgatgaacc
     3121 aatttttacc agtgtgttag aactggatat gaatgacgtt gaagtgagcc ttgcagggcc
     3181 aaaacgcccg caggatcgcg ttgcactgcc cgatgtacca aaagcatttg ctgccagtag
     3241 cgaactggaa gtgaatgcca cgcataaaga tcgcctgccg gtcgattatg ttatgaacgg
     3301 acatcagtat cagttacctg atggcgctgt ggtcattgct gcgataacgt cgtgcaccaa
     3361 cacctctaac ccaagtgtgc tgatggccgc aggcttgctg gcgaaaaaag ccgtaactct
     3421 gggcctcaag cggcaaccgt gggtcaaagc gtcgctggca ccgggttcga aagtcgtttc
     3481 tgattatctg gcaaaagcga aactgacacc gtatctcgat gaactgggat ttaaccttgt
     3541 gggatacggt tgtaccacct gtattggtaa ctctggaccg ttgcccgatc ctatcgaaac
     3601 ggcaatcaaa aaaggcgatt taaccgtcgg tgcagtgctg tccggcaacc gtaactttga
     3661 aggccgtatc catccgctgg ttaaaaccaa ctggctggcc tcgccgccgc tggtggttgc
     3721 ctatgcgctg gcgggaaata tgaatatcaa cctggcttct gagcctatcg gccatgatcg
     3781 caaaggcgag ccggtatatc tgaaagatat ctggccatcg gcacaagaaa ttgcccgtgc
     3841 ggtagaccaa gtctccacag aaatgttccg caaagagtac gcagaagttt ttgaaggcac
     3901 agctgagtgg aaggaaatta acgtcacacg atccgatacc tacggttggc aggaggattc
     3961 aacctatatt cgcttatcgc ctttctttga tgaaatgcag gcaacaccag cacctgtgga
     4021 agatattcac ggtgcgcgga tcctcgcaat gctgggggat tcagtcacca ctgaccatat
     4081 ctctccggcg ggcagtatta agcccgacag cccggcgggt cgatatctac aaggtcgggg
     4141 tgttgagcgg aaagacttta actcctatgg ttcgcggcgt ggtaaccatg aagtgatgat
     4201 gcgcggcacc ttcgccaata ttcgcatccg taatgaaatg gtgcctggcg ttgaaggggg
     4261 gatgacgtgc catttacctg acagcgatgt cgtctctatt tatgatgccg caatgcgcta
     4321 taagcaggag caaacgccgc tggcggtgat tgccgggaaa gagtatggat caggctccag
     4381 ccgtgactgg gcggcaaaag gtccgcgtct gcttggtatt cgtgttgtga ttgccgaatc
     4441 gtttgaacga attcaccgtt cgaatttaat tggcatgggc atcctgccgc tggaatttcc
     4501 acaaggtgtg acgcgtaaaa cgttagagct taccggggaa gagaagattg atattggtga
     4561 tctgcaaaat ctacaacccg gcgcgacggt tccggtgacg cttacgcgcg cggatggtag
     4621 ccaggaagtc gtaccctgcc gttgtcgtat cgacaccgcg acggagttga cctactacca
     4681 gaacgacggc attttgcatt atgtcattcg taatatgttg aagtaacgag tatttgcttg
     4741 ccggttattt tgcttccggc aagcaaatta attacacaat gcaagagggt tatttgttca
     4801 gcaaatgacc cattttctcg gctttggtat cgagataatg ttcgttattg gggttacgac
     4861 ctacaatcaa cggtacgcgt tcaataatat taatccctgc ttcggtcaga atttcgactt
     4921 ttttcgggtt attggttaac aagcggactt cattgacgcc aaggagtttg aacatatcag
     4981 cgcaaagagt gaagtcgcgc tcatcagcgg cgaagcctaa ctggtggtta gcctctacag
     5041 tatcgtaacc ttgatcctgc agtgcgtaag cgcggatttt attcagcaga ccaatgttac
     5101 gaccttcctg acggtgatac agcaaaatac cacggccttc ctcggcaatt tgcgtcaatg
     5161 ccgcttcgag ctggaagcca caatcgcagc gcaagctgaa cagggcgtca ccggtcagac
     5221 attcggaatg gacgcgcgca agtaccgggg tatgcccgga aatatcgcca tagactagcg
     5281 cgacatgatc gtgtccggtt gccagttctt caaatcccac catcaggaaa tcgccccatg
     5341 gggttggcag tttggcttct gccacacgtt taagctgcat gaaattctcc agataatgct
     5401 ggttctgtat tggcttattt tgccataacg agaaggggtt cacctaatca cgacgcgtcg
     5461 atcgttcacg gaatggcaca aatctgtcaa tttttcctgg aactggcatt ttcagttatg
     5521 attgtgggac ttatcaaaaa ggagaggcca tgcgttcgat tgccagacgt accgcagtgg
     5581 gagctgcact attgcttgtc atgccagtag ccgtatggct ttctggctgg cgttggcaac
     5641 ctggagaaca aagttggtta ctaaaagcgg ctttttgggt tactgaaact gtcacccagc
     5701 cctggggcgt cattacacat ttgattttat tcggctggtt tctctggtgt ctgcgttttc
     5761 gcattaaggc tgccattatg ttatttgcca ttctggcggc ggcaatcctt atgggacaag
     5821 gcgttaaatc ctggatcaaa gacaaagtcc aggaaccacg accttttgtt atctggctgg
     5881 aaaaaacaca tcatattccg gttgatgagt tctacacttt aaagcgagca gaacgcggaa
     5941 atctggtgaa agaacagttg gctgaagaga aaaatatccc acaatatttg cgttcacact
     6001 ggcagaaaga gacggggttt gcctttcctt ccggtcacac gatgtttgct gccagttggg
     6061 cactgctggc cgttggtttg ctgtggccgc gtcggcgaac gttaaccatt gctatcttgt
     6121 tggtctgggc aacgggagtc atgggaagcc gcctgctgct cgggatgcat tggccacgcg
     6181 atctggttgt agctacgttg atttcgtggg cgctggtggc ggtggcaacg tggcttgcgc
     6241 aacgaatttg tgggccatta acaccacctg cggaagaaaa tcgcgaaatt gcacaacgag
     6301 aacaagaaag ttaacgctgg ttgattttcc gaatttagcc cttaaatcat caacaatgcg
     6361 cgtggatgcc atttcgcaga cggcgcgaaa atggtacttt aaagggctat tgcggtaagt
     6421 tgaccataat ttattcgccc taaccacata acgggaagta atgtgaaata tttactcatt
     6481 ttcttactgg tgttagcgat cttcgtgatt tcggtcacgt tgggtgcgca gaacgatcaa
     6541 caggtgacgt ttaattatct gttagcgcaa ggggagtacc gtatttccac attgctggcg
     6601 gtattgtttg ctgcggggtt tgctatcggt tggttgattt gtggcctgtt ctggctgcga
     6661 gttcgtgttt ccctggcgcg cgctgaacgt aaaataaagc gactggaaaa ccagctttca
     6721 cctgcgactg acgtggctgt agcgccgcac tcgtctgcgt cgaaggaata actttctatg
     6781 ctggagttgt tgtttctgct tttgcctgta gccgctgcct atggctggta tatgggccgc
     6841 agaagtgcgc aacaaaacaa gcaagatgaa gccaaccgct tgtcgcgtga ttacgtagcg
     6901 ggggttaact tcctgcttag taatcaacag gataaagcgg tagacctgtt tctcgatatg
     6961 cttaaagagg atacaggtac cgttgaagcc caccttacgc tcggaaacct gttccgttcg
     7021 cgtggcgaag ttgatcgcgc tattcgcatc catcagaccc taatggaaag cgcctcgctg
     7081 acctatgaac agcggctttt ggcgattcaa caactggggc gtgattacat ggctgccggg
     7141 ttatacgacc gcgcggaaga catgttcaat cagctgaccg atgaaactga cttccgcatt
     7201 ggcgcgctgc aacaactgct acaaatttac caggctacca gcgagtggca gaaagcaatt
     7261 gatgttgccg aacgcctggt gaagctgggt aaagataaac agcgcgtcga aattgcccat
     7321 ttctactgtg agttagctct gcagcatatg gccagcgacg atctcgatcg tgccatgact
     7381 ttgctaaaaa aaggtgcggc ggcagataaa aacagcgccc gcgtatccat catgatggga
     7441 cgcgtgttta tggcgaaagg agaatacgcc aaagccgtcg aaagtttgca acgtgtcata
     7501 tcccaggaca gagaactggt cagcgaaacg ctggaaatgc tgcaaacatg ctatcagcag
     7561 ttgggtaaaa ctgccgaatg ggcagaattc ctgcagcgcg cggtggaaga gaacaccggt
     7621 gccgatgctg aattgatgct tgcggatatc atcgaagcgc gcgacggtag tgaggccgca
     7681 caggtctata ttacacgcca gcttcagcgt catccgacca tgcgtgtgtt ccataagtta
     7741 atggattacc acttaaatga agcagaagaa gggcgtgcca aagagagtct gatggtgctg
     7801 cgtgacatgg ttggcgagaa ggtacgtagt aagccccgtt atcgctgcca gaaatgtggt
     7861 tttaccgcat acaccctcta ctggcattgt ccgtcttgtc gggcctggtc aaccattaaa
     7921 ccaattcgcg gtcttgatgg cctgtaattt taaaaaaatc cgactttagt tacaacatac
     7981 taattattga tgttctattg tgctccggca ccgacggcgc ggaaaagcct gccagaggag
     8041 aaatcgcaac tgttaatttt ttatttccac gggtagaatg ctcgccgttt acctgtttcg
     8101 cgccacttcc ggtgcccatc atcaagaagg tctggtcatg acgttaactg cttcatcttc
     8161 ttcccgcgct gttacgaatt ctcctgtagt tgttgccctt gattatcata atcgcgatgc
     8221 cgcgatggcc tttgtcgaca agatcgaccc acgcgattgt cgtctgaagg tcggcaaaga
     8281 gatgtttaca ttgtttgggc cacagtttgt gagcgaactt caacagcgtg gttttgatat
     8341 ctttcttgac ctgaaattcc acgatattcc caacactgca gcacacgctg tcgctgctgc
     8401 ggctgactta ggcgtgtgga tggtgaatgt tcatgcctcc ggtggggcgc gtatgatggc
     8461 cgcagcgcgt gaggcactgg ttccgtttgg caaagatgca ccgcttttga ttgctgtgac
     8521 agtgttgacc agcatggaag ccagcgacct ggccgatctt ggcgtgacac tgtcacctgc
     8581 agattatgca gaacgtctgg cggcactgac gcaaaaatgt ggccttgatg gtgtggtgtg
     8641 ttctgctcag gaagctgtgc gctttaaaca ggtattcggt caggagttca aactggttac
     8701 gccgggcatt cgtccgcagg ggagtgatgc tggtgaccag cgccgcatta tgacgccaga
     8761 acaggcgttg tcggctggtg ttgattatat ggtgattggt cgcccggtaa cgcaatcggt
     8821 agatccagcg cagacgctga aagcgatcaa cgcctcttta cagcggagtg catgatgagt
     8881 gattccaaca gtcgtctggt ctactcaacg gaaaccggac gtattgatga gccaaaagcg
     8941 gcccctgtac gccctaaagg cgacggtgtg gtgcgtattc agcgtcagac cagtggacgc
     9001 aaaggtaagg gcgtttgcct gattaccggt gtcgatctcg atgatgccga actgacgaaa
     9061 ctggcagctg aactgaagaa aaaatgcggc tgcggcggag cagttaaaga tggaattatt
     9121 gaaattcagg gcgataagcg tgatttatta aaatcaatgc ttgaagcgaa agggatgaag
     9181 gtaaaactcg caggcggcta acataaaaag ccacggatat atctgtggct ttcgaatatt
     9241 ttactgtgtg tattattcag ttatttcgta ctgaacagaa tcaagcgtat tataattgcg
     9301 agtcgataat cgacatttat ttacgattat ttaccgacct ggtgaccaat aacaccaccg
     9361 acggctgcac cacctaatgt acccaacgta ctgccatcgg tcagtactgc accgcctaat
     9421 gcccctgcac ctgcaccgat tgcggtgttg cggtcccgtt tagaccagtt agaacaggca
     9481 ctcagagaca ttgccagggt aattgccaga acagccgcgg tcattttttt gctcgttaca
     9541 aacataatac tctctcctga atttatgatt cacggaagta agctctctat aactataata
     9601 gctaagaata agccaggtga aattattccg tgaaatccgc tcgcgcgcag ggttatatca
     9661 cgcaggtgat aatgacttcc tgttatatcg ctgataataa ttttatatct tgagagtgtt
     9721 aataacaggt aaatagtctt aattatcaac caggaatcat cttagagcgg atgatttgcc
     9781 aaactgcaaa tcatccgtag agaagggaaa tggttaaatg tcaacgacgt gaacggtgag
     9841 tttactgtgc tcaagatgca tcagatcgct ggcgcgtatt ttcgaatcgg taatcacgcg
     9901 attgaatcgc tcaacggggc cgatggagta tgaatgtaca gcaccaaatt tcgagctgtc
     9961 ggtcaggact atcgcctcgc actctttttc cagcacggca ttgaccacat cggtacgcat
    10021 catatcgcga ccggtaaacc cagtttcagg ttgccagcca tcaataccaa taaatgcttt
    10081 gctgaaatgc acctgttgga tgcactggcg tgtcaaaggg ccaaccatac tttcgctttt
    10141 tttctggtac acgccaccga gcaaaatgac ttcacaaggc gcgtctttca gcaaatgcgc
    10201 gatgtagctg ctgaccgtga tgatagtggc atttttcttc tgctcgccca gagtcctggc
    10261 gagcagggca ttgctgctac cattttcgat aaagatggtt tcgcccggtt gaaccagtga
    10321 cgcggcaaac tcggcgagtt cacgcttcag cgtatagttg ctcatcatac gggtttcgac
    10381 gtcatcacta tcaagcgaaa ctgcaaaacc atgtgcacgt cggaggtaac tcagtttttc
    10441 gagggtgttg agatcctggc gaatggtaac ttcagaaact ccagtggctt ttgccagatc
    10501 ggttacgcta acctgaccct ggtcaatgac catctgtaga atagtttgtt gtcgggaatt
    10561 catcgctgta attcttataa cgttataata ctaaataaaa aaatactgac gcgccgggca
    10621 tcctcaaata ggaaaacctg aaggcacgat tgttagatct cccacggtgc cttcgggatg
    10681 tctggcgtag attcagcaac ctggcgcaag agttctgctt taagtatctc gagattatgg
    10741 atcgcagagt ggtaatcgcc tgccacaagg atatctaaca caatatcaat acgttcggca
    10801 acgcgctggg catcaaattc agacatcaag gacatcctta tagcaaaagg ggaaaaatga
    10861 ataatgcaaa aattgccgct aaaagagaag tgtttaacag caacggctaa ttatcatccg
    10921 ggaatacgat atataatgac ggaatatagc gctaagtata tatattcatc tacttatgcg
    10981 cgcttcagat agcgtttata ccagcgttcg aaggcaacgg cgggcatcgg tttggcaaac
    11041 aaaaatcctt gtcgctcatt gatcccgttc ttggttaaaa aagcatcttc ctttgcactt
    11101 tctacacctt cggcgatcac ctgaagattc aatgcctggg ccacagcgac gatcgcccgg
    11161 accagtgatt gcgagacagg ctgtttgtga atatcccgaa caaaaacctg gtcaagtttg
    11221 atggcatcga tcggaaagcg cgccagttgc gaaagtgaag agtagccggt accaaaatcg
    11281 tccagatgaa cttgcgcacc taatcggcta aattgttgaa taacagacag tgccagttca
    11341 tcattctcaa tcaggcagct ctccgtcagt tcaacatcta tagggcagta ttcaaaattg
    11401 agttcctgga gaacctgttt cagggcggtg aaaatggttt gatcggcgag ctgacgtgca
    11461 gaaatattta ccgccactcg caggttaatg cctttatccc gccacttcgc tacctggcgt
    11521 acgacatcga gaatcaccca acggcctaaa ggcacaatta gccctgattc ttcggcgtag
    11581 gaaatgaagt ccaacggtgg aatcaaccca cgttcaggtg actgccaacg tactagtgct
    11641 tccagactgc gcacttcgcc acgccaggtg attttcggtt gatagtgaat aaccaactga
    11701 tcgttttcca gtgctttacg caagttggta tccagccaga gatattcaaa tacccgctga
    11761 ttcatttctg gggtaaaaac gcaaaattgt cctcgtccgc cttccttcgc tgtgtacatt
    11821 gctgtgtcgg cgtgacgaat aatagccgcg ctgtccgaac catgttcggg agagagtgaa
    11881 atacctactg aacagctggt ataaacttca attaaaccaa tgcgaaaggg gagtcgtaag
    11941 cgggtcaaaa ttcgtgatgc caatgcttcc agcgcgcttt gtgaggtatt ggatgccagt
    12001 accagaaact catcgccacc tggacgcgcc aatacttggt catgttcgag acagcttaaa
    12061 atagccaatg acacgtcgcg taataactgg tcaccaaaca aatgcccata ggcgtcgttg
    12121 acctttttaa aattatccaa atcaagatac acaaccccaa ctttattgtt atctgcctga
    12181 ttaatagcgt gatcgattaa ctcctgcatc gcgttacgat tcggcagtcc ggtgatactg
    12241 tcggtatttg ccagaatacg cagtcgctcc tgagcgcggc gctcttcggt aatgtcggtg
    12301 ccggaacaga ttaaaaaaat ctcgtttttg ccactgccgc tgtggacaaa tttattgcga
    12361 aacagaaaca gccgctggcc tttacgtgtt ggtatccata gttcgacttc atatgcattg
    12421 ccgcttcgaa aaaatacacg gttattgcgc ctggatgccg cagcttcacg acgactcata
    12481 aacagtttaa acatgctttg cccaatgacg tcgtgttctt tcaagcctgt gtaatcttca
    12541 cataaccgat taaagcgctg gatattcccc cggctgtcga gaatcactat tgcggaatgt
    12601 gcttcagaaa ctacctgctc agcaaaggaa agcccatgtg ataaatcacg agcaaccgcc
    12661 ggtgtgtcat gccatgcaga cgcgttgcct ccccattctc gtttattgat ttttcgtcct
    12721 accagatgca cagaaagatc gctttcatcg acggtcagac tcatcatcag gctggaggtg
    12781 atgaccgtca tttccctgat gcgagcagcc tgttcggcag atagttgcaa aatacgatcg
    12841 ggttctgtga tttcggtggt agaaaagcgc aaaacatcgc tgctttccgt caaccgccag
    12901 tatggattgt gcgacccgag aaagttgtac aacgttgtgg actccctaac ggttttcatg
    12961 atgcatatcc tccggttaac agcggagtcc gcagttaaat aaaaggaaca acatgactta
    13021 catgaaatta acggcggcta aacacattgt ttttttatat atttatttgt aatccagttt
    13081 tggaaaaacg ccagttttca aacgaaagtc agttaaaaat ctgccgggat atgacgaagg
    13141 ttgagcgggg aaataaacgg cccatccatg aggaatgggc cgtgaaagga gattacgcga
    13201 ctgggcgcgc aataatgctg cgggtttcca tgcggacttc ggcaatggtg acgtcaataa
    13261 cgtcagtcac tttgtaagcc gtttcacctt taatttgtac agtgccgttt tcctggctgc
    13321 aaaccagttc atcgcgcaca gcgtgtaaga aaggtgctgg aataaaggcg atagcgccgt
    13381 tatcaaccaa acgaacacgc atgccgccac ggctgatatc gacaatttcc gccgcgaaac
    13441 gggtgtcggt cccggctttg tctttcagga agcgtgcgta taaccagtcg ccaacatcac
    13501 gttctgccat ccggttgaga cggcgacgct cggccatttg gacagtgatt tcatcctgtg
    13561 gacgcgtcgc agtttcgcct ttgataaccg ctttcagcag acggtggttg atcatgtcgc
    13621 catatttacg gatcggcgaa gtccaggtgg cgtatgcttc cagaccgagg ccaaagtgag
    13681 gaccgggttc agtgctaatt tcagcaaatg actggaagcg acgaatgcgg ctgtcgagga
    13741 aaccagttgg ttgcgcatcc agttcacgac gcagtttgca gaaaccgtcc agcgtgagca
    13801 cttcttcagc atcgacatgc agaccgtgcg ttttcagcaa cgctgccagc gcgtcggcat
    13861 tcgccggatc aaagcccata tgcacgttat agatgccaaa accgagctta tcgcgcagta
    13921 cgcgggccgc acaaatgtta gcggcaatca tcgcttcttc gacgatacgg ttggcaatgc
    13981 gacgaggctc ggcgacgata tccagcacct cacctttttc accgagaata aagcggtaat
    14041 ccgggcgatc tttaaacacc agtgcgtggt tatgacgcca ctcgccgcgg cgttggcaaa
    14101 tttgtgctag caaacggact tgttcggcaa ttgcttcgct ttcaggctgc cagtcaccgg
    14161 tattttccag ccagtcagaa acctggtcat acaccagctt cgctttggat tcgatggtgg
    14221 cggcaaagaa ttcgatatta tcttcaatgg tgccatcagt ggagagcgtc atgcggcatg
    14281 ccagtaccgg gcggacttca ttggcgcgca gtgagcagag atcgtcagaa agctcgcgag
    14341 gcagcatagg gatgttgaag ccaggcagat agttggtgaa tgcgcgaatt ttcgcggctt
    14401 tgtccagctt gctgccttca gcaatccacg cggttggatc ggcaatcgcc acaatcagct
    14461 gaagtttgcc atccggtaac gccttggcga aaagggcgtc atccatatct tctgtgctgg
    14521 cactgtcgat ggtgacaaaa tccagcgcag tcagatcttc acgaaccaga ccttcatcga
    14581 gcatttcggt agcgacgccg tctggtgctt ctttttccag attatggcgc gcgagggtaa
    14641 cccaccacgg tacaaagtga tcgtcaccaa aagtgatgta ttgtgtcagt tcagcataga
    14701 aagaacgatc gcctttcagc ggatgacggc gcatttcggc aaccgcccag tcgccttctt
    14761 taaactcgtg gttcaggcca cgggctgcgc ggcaaggaat ggcgtctttt aagagtggat
    14821 gatcaggaac gatggccaga cggtcatttt tgccctgaac tttacccacg aaacgagtca
    14881 ggaacggttc aaccagttct tctggctctg cggattcacg ttctttttca ctgtggataa
    14941 ccgcgataat tcggtcgcca tgcatgactt ttttcatctg cggcggcgga atgaaataac
    15001 ttttttgcgc gtcgacttcc aggaagccaa agcctttttc tgtggctttt accacccctt
    15061 cagcgcgtgg cgtctgggaa tgcagttgct gtttaagctg cgctagcagc gggttgtcct
    15121 gaaacataat tgtctatttt ggtggccatt agagcggctg acagttttac gcgaatctgt
    15181 ctgacgcggc aaggttaata tgtctcaccc aacgcgattt ttaagcgatt tatccagcca
    15241 caaagcccgc tccataccag cagattaata atctgcgttg atgatttttc gtgttcgagt
    15301 aatgggctga actgggcggc gctaaagcga tcgggtgctc gggtcagcca ttgtatcgcc
    15361 tgaacggtag cgcgctcaac agaatgacgg tcagcccatc ggttaatttc attttcgccc
    15421 ttgcgcagaa gggggctgat ttttttatat tcatttgatg aattcataca ggcggcaaaa
    15481 caagcaggac tgccatttat ccgcgcgcat aacaacgccg caagctgatt atccggctgt
    15541 gtattgaccc gtgtgctgtt taacaacttt tcgagaaggg aaagtgaaat agggtggaga
    15601 gagagtgtgt tggccagttg gcaaagttct gcagttgaac tttttgtgtg ctcttcaggg
    15661 agtttttcca cctcatagct gcttcgccag cgtaatgatt catcagcaaa cagtgacgcg
    15721 tcggcgtagt tttgtatctc cagcccgggt aaccagcgta ccggatgccc gagataagcc
    15781 tgaaatgtcg caattgtccg cgcctgaaag ccaataaaac caatgatttg gttaatgatc
    15841 acacagtcac agacagtaag accaacgtca ttcagatgct ggcgcgatcg gttgtcgata
    15901 atagaaggcg agctagccag ttggcgggca tattgcgtga tttgtgccaa ccgatgatta
    15961 ctttccctgg aggaatcagg cccggggagc ggcgtaagtc gcgccgaata gtagttacat
    16021 aaccgttgga cgccgcaaac ctgagcaacc gtcagggccg tcgacaagcg ttcataagcg
    16081 gtgaaggtct gtgaacgggt cacggtaagc tggtcaggga acaactctac tgcaagaacg
    16141 cgcgcaggga ccagccatga aaggaagggt gccagcgttt cttcatcaag gatcacgtcg
    16201 agcagaaagg gatcgctgac ctttgcggct tccgggacca gaggcagaac gtcatactcc
    16261 gcagtactgg attgcgtttc atgataccag tggcttttgc cggtgatgtg gcgttgttcc
    16321 atgggcgctc cttggtcgta aaggaaatcg ttatcctgac gcaaggcggg aaggggagaa
    16381 agacggatcg gcgataacaa atatcagaaa ggtataacag agataacggg cggcagaacg
    16441 ccgcccaata tttaccaaca gaacgattat ttcagttcga gttcgttcat tgcagcgatg
    16501 ctgaaaccgc cgtcaacgtg gaccacttca ccggagatac cggcagagag atcggagcac
    16561 aggaatgccg cggagttacc cacatcttca atagtaacgg tacggcgaat cggggtaacg
    16621 gcttcgcaat gagccagcat tttgcggaag tctttgatac cggaagccgc cagagtacgg
    16681 atcggaccag cagagatggc gttaacacgc acaccttccg gacccatcgc gttcgccata
    16741 tagcgcacgt ttgcttccag agacgctttt gccagaccca taacgttgta gttcgggata
    16801 gcgcgctcag cgccaaggta ggaaagggtc agcagggcag aacccggatt cagcatggag
    16861 cggcaagctt ttgccattgc aacgaagctg taggagctga tgtcgtgggc aattttgaag
    16921 ccttcacggg taacggcgtt aacatagtca ccatccagct gatcgccagg tgcaaaacca
    16981 atagagtgta cgaaaccgtc aaatttcggc caaactttcc ccagttcagc gaacatggtg
    17041 tcgatgctgg tatcttcggc aacatcgcac tgcagaacga tgtcagaacc caattgagcg
    17101 gcaaattctt ctacgcggcc tttcagtttg tcgttctggt aggtgaatgc cagttcagct
    17161 ccttcgcggt gcatcgcctg agcgataccg taggcgatgg acagtttgct ggcaacaccg
    17221 gttaccagaa tgcgcttacc ggaaagaaaa cccatagctt taatccttat tgttgatgct
    17281 tgttgtgcct gaaaatcagg cgattcgtcg ttttagtaaa cagtacgaac agataaacgg
    17341 ttattataat caacctggct gtgggtggct atagttgcgc tttctgtagc ggagagaagc
    17401 tgcggtacag agcattttgc gttatctact cttgacgcct gcaaggggta ggttggggaa
    17461 atatcttata gaatctgact atgaaactat tcgtattagc tccgataagt ttgatgatga
    17521 aaacagaact ggcgacaaca agtcgctgcc tttctgatga attgacccca acttaatatt
    17581 gtcaactatc ttcacagaat ctccacaatc tcttcattat cagaatatgc accatgaata
    17641 agataaaaat actttccatt actttggaga aacgtataat cagcaaaaat gagaacgatc
    17701 attctcacca agtgataggc caggagacag aaatgaccag caagctggag atacgccaca
    17761 aacagcgtca ggatgaaatc attaacgccg cccgtcggtg ttttcgtcgc tgcggatttc
    17821 atgccgccag tatgtcgcaa attgccagcg aagctcagct tagcgtgggt cagatatatc
    17881 gttacttcgc caacaaagac gccattattg aagagatggt tcgtcgtatc atcgactttc
    17941 gtattgccca aatggacatt gacgcgcgta ccgatcactt cccggaagtc cttgccttgc
    18001 gtaaatcctt aaatgaggac gacgacgcgc tgatgctgga agtggctgca gaagccacac
    18061 gcaacccgcg ggttatggca atgcttgaag aagctgacgc cagaatgttc gctaacggtt
    18121 gtgcacatat gaaaagaatg catccccatc ttagcgacga gcacattcgt tgctgcgttg
    18181 aagtgtttgc taccatgatg gaaggtacgg tttatcgtcg tttaacaccg caaaaaagcg
    18241 atccacaaca tctgcaggaa atatatcagg atattgttag tatgttgata aataaataag
    18301 aacagaatta accaatagcg ggggatatta atccccctta tttcattgcg aaattcattt
    18361 cgcgatgcgg gttcgttata actgaatatc cctgacatat cctcattaat ttcaatgagg
    18421 agggtatttc tcaccctgga gaaaataatg aagtatatag caacatctgt agtggcaatg
    18481 ctgctcttat cgggttgtga taatacgcaa agtaacaatt catccccgtc agaaacagaa
    18541 gtcggcgttg ttacgctcaa atctcaaccg gtttcggtag tcagtgaatt aaccggacgc
    18601 accagtgctg cgctcagtgc tgaagtacgt ccgcaggttg ggggaattat ccagaaacgc
    18661 ttatttaagg aaggtgatct ggtcaaggct ggacagcctc tctaccagat tgatgcggcc
    18721 agttatcagg ctgcatggaa tgaagcccgg gcagcattac aacaagcaca ggcactggta
    18781 aaagccgatt gccagaaagc gcagcgttat gcccgactgg tgaaagagaa tggtgtttca
    18841 caacaggatg ctgatgatgc tcagtctacc tgtgcacaag ataaagccag tgtagaggcg
    18901 aaaaaagccg cactggaaac tgcgcgcatt aatcttgact ggaccacggt aaccgcaccg
    18961 atttcggggc gtattggtat ttcgtcggta acccctggcg cactggtgac agcgtcgcag
    19021 gatacagcgt taacgactat tcgtggtctg gatacaatgt atgtcgacct cactcgctcc
    19081 agtgtcgatt tattacgtct gcgtaaacag tcactggcga ccaacagtga cactatgagc
    19141 gtctcactta ttctggaaga tggcacaacc tacagcgaaa aagggcgtct ggaactcacc
    19201 gaagtcgcgg tggatgagtc taccggttcg gtgacattac gggcaatttt ccccaatcca
    19261 caacagcagt tattaccggg aatgtttgtt cgcgctcgtg tcgatgaagg cgtgatggaa
    19321 aacgctattc tcgcgcctca acagggcgtt acgcgcgatg ctaaaggcaa tgcaactgcg
    19381 ctggtggtga ataaagacaa taaagtagag cagcgaacgc tcgaaacggg agaaacgtat
    19441 ggtgataaat ggctggtgct gaacggcctg cacaacggcg accgactgat tgttgaaggt
    19501 tctgccaaag tcacttcagg gcagaccgtc aaggctgttg aagttcaggc taatggaggc
    19561 aatgcctgat gttttcgcgc ttttttgttc gccgcccggt ctttgcctgg gttatcgcca
    19621 ttttgattat gttggcgggg attctggcta ttcgcacatt acccgtcgcg caatatcctg
    19681 acgttgcacc gccgaccatt aaaatttcag ccacttatac tggtgcttct gccgaaacgc
    19741 tggagaacag cgtgactcag gttatcgagc aacaactcac tgggcttgat aatttactct
    19801 atttcagctc aacaagtagc tctgatggtt cggtcagtat taatgtgacc tttgaacaag
    19861 gtaccgatcc agatactgca caggttcagg tgcagaataa aattcagcag gcggagtcgc
    19921 gcctacccag cgaagtgcag caaacgggtg ttacggtgga gaaatcacaa agcaactttt
    19981 tgctgattgc tgccgtgtat gacaccactg acaaagcctc cagttcggat atcgccgact
    20041 ggctggtcag taacgttcag gaccccctgg cgcgtgttga aggtgtgggg agtctgcaag
    20101 tctttggcgc ggaatacgct atgcgcatct ggctcgaccc ggccaaactg gcgtcttact
    20161 cgctgatgcc ttcagacgtg caaagtgcta ttgaagcgca aaacgtgcag gttactgccg
    20221 ggaaaatagg ggcattgcct tcaccggata ctcagcaact gaccgcaacg gtacgtgcgc
    20281 agtctcgttt gcagacggtg gatcagttca aaaatattat cgtgaaaagc cagtcagacg
    20341 gcgcagttgt tcgtataaaa gatgtggctc gcgttgagat gggcagtgaa gattataccg
    20401 ctatcggcaa acttaacggt cacccgtctg ccggggttgc tgtaatgctt tcgcccggtg
    20461 cgaatgcgct gaatacggcg acgctggtca aggataagat tgccgaattc cagcggaaca
    20521 tgccgcaggg atacgacatc gcatacccga aagacagcac tgaatttatt aaaatctccg
    20581 tagaggacgt aattcagacg ctgtttgaag ccatcgtatt ggtggtttgc gtgatgtatt
    20641 tattcctgca aaacctgcgt gccacactga ttccggcgtt agctgtcccc gtcgttttgc
    20701 tgggcacatt cggcgttctt gcgttgtttg gctattcgat taataccctg acgttgtttg
    20761 caatggtgct ggcgatcggc ttgctggtgg acgatgccat tgtggtggta gagaacgtcg
    20821 aacgcattat gcgtgacgaa gggttgcccg cgcgtgaagc cacggaaaaa tcaatgggcg
    20881 agatttctgg cgcactggtt gccattgcgc tggtgttgtc agccgtattc ctgccgatgg
    20941 ccttctttgg gggatctacg ggggtaattt atcgtcagtt ctccatcacc atcatctccg
    21001 caatgctgct ttccgtggtg gtggcattga ccttgactcc cgccctgtgc ggttccgtcc
    21061 tccagcatgt tccgccacat aaaaaaggct ttttcggcgc atttaaccgc ttttatcgcc
    21121 gtactgaaga taaatatcag cgaggcgtaa tttatgtcct gcgccgtgca gcccgaacga
    21181 tggggcttta tgtcgtgctg ggtggtggaa tggccctgat gatgtggaaa ctgccgggca
    21241 gtttcttacc cactgaagac cagggcgaaa tcatggtgca gtacacgctg ccggccgggg
    21301 cgaccgctgc ccgtacagca gaagtgaatc gccagattgt tgactggttc ctgattaacg
    21361 agaaagcaaa taccgatgtc atctttaccg ttgatggttt cagttttagc ggcagcggac
    21421 agaacaccgg gatggcgttt gtttcgttga aaaactggtc tcaacgtaaa ggggcagaaa
    21481 acaccgccca ggctatcgcc ctacgggcta ccaaagagct gggcacaatt cgtgatgcca
    21541 cggtatttgc gatgacgccg ccagccgttg atgggctggg gcaaagcaat ggttttacgt
    21601 ttgaattgtt agctaacggt ggaaccgatc gtgaaacact gctgcaaatg cgtaatcagt
    21661 tgatagaaaa agcgaatcaa agtccggagt tacattctgt acgcgccaat gatttgccac
    21721 aaatgccgca attgcaggta gatattgata gtaataaagc ggtgtcatta gggttgagtt
    21781 tgaatgatgt caccgacacc ctgtccagtg cgtggggcgg tacttatgtg aatgacttta
    21841 ttgaccgtgg tcgtgtgaaa aaagtctaca ttcagggcga cagcgaattt cgttccgcgc
    21901 cgtcagactt aggtaaatgg tttgtgcgcg gtagcgataa cgccatgaca ccattctctg
    21961 cttttgcgac cacccgctgg ctgtatggac cggaaagact ggtgcgctac aacggctcgg
    22021 cagcctatga aattcagggc gaaaacgcga ctggctttag ttccggcgat gcaatgacaa
    22081 aaatggagga actggcaaac agtcttcctg cgggaacaac atgggcctgg agtggtttgt
    22141 cattgcagga gaaactggcc agcggtcagg cattaagtct gtatgcagtt tctattctgg
    22201 tggttttcct ctgccttgca gcactgtacg aaagctggtc agtcccgttc tcggtcattc
    22261 tggtgatccc tctggggctt cttggcgcgg cgctggcggc ctggatgcgt gatttaaaca
    22321 acgacgttta cttccaggta gcgctattaa ctactatcgg cttgtcatcg aaaaacgcca
    22381 ttctgattgt ggaatttgct gaagcggcgg ttgcagaggg ctattccttg agtcgtgcgg
    22441 cattacgcgc ggcgcagact cgtttacgcc caatcatcat gacctcgctg gcgtttattg
    22501 cgggggtaat gccgctggcg atagcaaccg gcgcaggggc gaacagccgc atcgccattg
    22561 gtacgggcat tattggcggc acgctgaccg ctacattact ggccattttc ttcgttcctc
    22621 tgttttttgt actggtgaaa cgtttgtttg ccggtaaacc gcgccgtcag gagtaagtta
    22681 tgttgcgtcg ttctcttatt tttctggtgt tgttgagtgc gggatgtgtc tcgctcgatc
    22741 ctcactacag tacgcctgaa tccccaatcc cggcgacctt gccgggagca cagggccagg
    22801 ggaaggcaat cagccatgac tggcagcagg tgatccacga tccccgattg cagcaggtag
    22861 taaccattgc actgaacagt aaccgcgatg tgcaaaaagc gattgctgat attgactcag
    22921 cacgggcgct ctatgggcaa actaatgcgt cgttatttcc gacggtgaat gcggccttaa
    22981 gttcgactcg cagtcgttcg ctggcgaatg gtacgggaac aactgccgag gctgacggca
    23041 cggtgtccag ttatacgcta gatctgtttg gtcgtaatca gagtttatct cgggcggcgc
    23101 gggaaacctg gcttgccagt gaatttacag cccagaacac tcgtttaacg ctgattgctg
    23161 aaatcagcac cgcctggctg acactggcgg ccgataacag caacctggcc ctggcaaaag
    23221 aaacgatggc cagtgcagaa aactcattga aaattatcca gcgccagcaa caggttggca
    23281 cggcggcagc gacagatgtc agtgaggcga tgagtgttta tcagcaagcg cgcgccagcg
    23341 ttgccagcta tcagacacaa gtgatgcagg ataaaaatgc gctgaactta ctggcaggta
    23401 caacgcttgc ggaaaatctg ctaccgggaa cactggaaag cctgccggaa caaatgataa
    23461 gtctggttcc tgcgggagta tcatctgatg ttctgctgcg tcgcccggat atccaggaag
    23521 ctgaacataa tttgaagagc gccaatgcag atattggcgc ggcgcgggct aactttttcc
    23581 cgacgatttc gctgactgcc agtgccggtg ttggcagtga cgcattgtcg tcgttgttca
    23641 gccacggaat gcagatctgg tcatttgcgc catcggtcac attaccgctg ttcactggcg
    23701 gaagtaatct ggcgcagctt cgttacgcag aagcacaaaa gcgtgggctc attgccacct
    23761 atgaaaaaac cgttcagagc gcatttaaag atgtcgctaa cgcactggcg cggcggacca
    23821 cgcttgaaga gcaactggat gcacagcgcc agtatgttaa agctgagcaa caaacggtcg
    23881 atgttggctt gcgtcgctat caggcgggtg ttggtgatta cctgaccgtg ttgacggcgc
    23941 agcgcagctt gtggagcgca cagcaggagc tgctggcact gcaactgact gattttacaa
    24001 accgaatcac gctctggcag tcgctgggcg gcggtatgtc atcactcaag taaggaataa
    24061 tatggctaga gtctctcttt catgggcatt gattcttggt cttttagccg gtatcggccc
    24121 gatgtgtacc gatctttatt tgccggcttt gccggagatg tctgagcaac tggcggcaac
    24181 cacgaccata acgcaattaa ccctgaccgc atcactgatt ggtcttggcg tcggacaact
    24241 gttatttggc cctctgagcg acaaaatagg gcgtaaacgt ccgttaatct tgtcgttgtt
    24301 attgtttatt gtttcttcca ttttatgcgc gacaacgaac aatatttact ggctggtggt
    24361 ctggcgtttt attcaaggga tcgcgggggc gggtggttcg gtgctctctc gttctattgc
    24421 tcgtgacaaa tatcagggag taacgttgac ccagtttttt gcgctgttaa tgacggtgaa
    24481 tggcctggca ccggtgttgt cgccagtgct gggcgggtac attgttagca cttttgactg
    24541 gcgcacttta ttctgggtaa tggctgaaat tagcaccgta ctgttgctgg gctgcctgtt
    24601 atttattaat gagaccttgc cagaaaataa aaggggctca tcattgctat taaccggacg
    24661 aggcgtggtg cagaaccgcc gttttatgcg cttttgcctg attcaaagct ttatgctggc
    24721 tggtttgttt gcatatatcg gctcttcgtc gttcgtgttg cagaaggaat ttggctttag
    24781 tccaatgcaa tttagcctgg tgtttggcct taacggcatc ggacttatca ttgcttcatg
    24841 gatcttctcg cgcctggcgc gacggattaa cgcgatgaca ttgttgcgag gtggcctgat
    24901 agcggcaatt ttgtgtgcat tgctcacggt cttatgcgca tgggtacaat tgcccattcc
    24961 ggcactggtg gcattatttt tcaccatcgc attttgtagc ggcatcggca ctgttggcgg
    25021 ggcagaggct atgagtgcag tagggacgca ggaatctgga acggcgtctg cgttgatggg
    25081 gatgagcatg tttgtcttcg gcggtatagc cgcgccattg tcgggaattg gcggagaaac
    25141 actgttaaaa atgagtctgg caattacggt gtgttatacg ctggcattgc tggttgctct
    25201 caccagaatc gacaaccaaa agtaatgaac acctcatcct gaggggggcc tgtcaggatg
    25261 aggtgcaaaa cactaacggt ccttacgcca cgcatccgcc gtcaatgctt cgccaaaatg
    25321 accggcgatc agccgtttgg tgagttcatg cagcggcgat gccagcacat ctgcggtgct
    25381 gcctcgctcg acaacctcgc cctgatgcat caccagcacc tggtcgctaa tgtgcttcat
    25441 cattccgata tgctgggtaa cataaatata cgaaatgccc tgtttttcct gtaactccag
    25501 catcagatta atcaactgcg aacgcatcga catatccagt gaggcgaggg cttcatcggc
    25561 aataatgact tttgggcgca atatcagcgc gcgcgccaga cccagacgct gtttttgccc
    25621 gggtgctaac atgtgcggat agtaactgac gtgatccggt agcagcccaa ccatacgcat
    25681 cgtttcaata atctgtttgc gacgctgttc cggttccagg tcggtgttca ggcgcagtgg
    25741 aaaatccaga atttgcgaga tacgttgacg gggattcaac gaggtggaag gatcctgaaa
    25801 aatcatgcga atacgctgac tgcggaagga ataatcgcca aaatgcagcg gatgatcgtc
    25861 aatcaataac tcgccgctgg taggttctat cattcccgcc agcatttttg ccagcgtgga
    25921 tttacccgaa ccattctcgc cgataatcgc cagtgtctgg ccttcacgta gcgtaaagct
    25981 caagggtttt accgcttcta cggtctgacg acgaaaccag ccggtccggt agcggaacgt
    26041 tttacttaaa ttgcgcactt caagtagcgt ttcgatcatc tcactctttc tccatgttca
    26101 gcgggaaatg acaggcatag agatgatttt tcgcccccgt caaacgtggc gtcacaatgc
    26161 attctcgttg tgcatacggg caacgtggcc ccagacgaca accaatcggt aactgttcca
    26221 gcagcgggat agcgccgggc agcgtattga ggcgactttt atgcggcatc gcgctgccga
    26281 agtctggtat cgcgcggatc agcgcctggg tataaggatg gtgtggcatc gtcaccaact
    26341 ccttactcgg cgcggtttcc accgtttgac cgcagtaaag cacgttaatt ttatccgccc
    26401 attggctaag catttgtaag tcatggctga taagcaaaat agtggtattg ctgttttggt
    26461 tgagacgcgt cagcaggcga aagatttgcg cctgggttgt tggctccatt gagttggtcg
    26521 gttcgtcagc aattagcaga cgtggttgat tcgccagtgc aatggctatc atcactttct
    26581 gacattcacc ttcggtcaac tcatagggaa aactgcgcat cgcatctttg tgatctttaa
    26641 tccccacgcg gtgcagcagt tcaatggcac ggcgtttgcg ccagccaaaa cgctgccacc
    26701 aacggccttt ataggtccag gcagggatgt tttgcatcaa ctggcgaccc acacgttctg
    26761 aagggtcaag acacgactgc ggttcctgga aaatcatcga cacgttgtga ccaaccagct
    26821 tgcgccgttc gcgtgcggag agacgcagca aatcgatatc atcaaaacgc atacggtcag
    26881 cagtaacacg ccagttatct ttattcaccc cacaaatcgc tttcgcaatc aaacttttgc
    26941 cggaaccgga ttcgccaaca agaccgcgga tttcaccttc ggttaaggtc atacttacgc
    27001 ggtcgacggc tttaacccac tcatcaccgg ttttaaattc aatggtcagg ttacgaatat
    27061 cgagtaatgg cattattcca cccccgcaat aattgcacga cggacaccgt cgccaaggag
    27121 gttaaccaac agcacgctaa tcataattgc cgcacctggc agcatgacag tccatggggc
    27181 gacataaatc agttccagcg catcaccgag catcgcgccc cattcaggcg aggggagttg
    27241 tgcgccgaga tcgagaaagc ccagcgcggc gatatcgaga attgccatcg acagtgcgcg
    27301 ggtgatctcg gtgaccagcc cggcggtgat gttgggcatc acagcaaacc agagaatatt
    27361 cagcgttgat gcgccatcca gacgggcggc gataacgtac tctttttcca gttcgtcatg
    27421 caccatgctg taaatcgaac gtaccatacg cggcagcagc gccagccaga cagcaaacat
    27481 ggcgtgagac aaactcggtc cggcaaacgc tacaacgata attgccagca gcagcgaagg
    27541 gatcgccagt aaggtatcca gaatatggtt aagcaccgct gagcgtaggc cgtgggtcgc
    27601 cccggcgaag gtgcccagta ccaggccaca gatcgtcgcg gcgagcgtca ccacaaatgc
    27661 gccacctacg gttggcgcag ctccgctcag taaacggctt aaaacatcgc gtccgaggtc
    27721 gtcagtcccc aggaagaaag aaacttcgcc atagcgcgac catgacggcg gcaataattg
    27781 ataaccgaga aattgctggt cgatgccgta aggcgcaaac cagccgccaa aaatacacag
    27841 tacagccagc cccgcgcagc cgtacaggcc gaccatcgca gaggcgtcac tataaaattt
    27901 gcgccaggcg gtacgcagcg tgcccggcgg gcgcttttcg ctgtatacgc tatcgtaagg
    27961 catgattacc ttctttaaac ttatttgctt tgattcgttt tgctttagtt ttttctttca
    28021 tttcaatcac atgccatatt taggtgcttt attcttgttt gttgctattc gtttcttgta
    28081 gttccaattt aagaaaaatg tgtacactaa agtgtatacg aaacacgagg gtgtacacat
    28141 tgctcaccga tacaaaacta agaaagtctc ttggcaaaaa acgagacaaa atcgaagtgc
    28201 tttctgatgc acaagggctg aatgttcggt tatcaacttc cggcagtata acattttttt
    28261 accgctaccg atggaatggc aaggctgcac aactggcgat cggcagttat ccgtccattt
    28321 cattggctca ggccagggag cgtcgccagc agtttagaga ctggctcact gagggactgg
    28381 atccccgtcg tcaggttatg ctggaaaagc agaaaaagtc tcaggctatg accgttaaag
    28441 aagcatttga ttactgggag caacattatt gtattcccga aggtctgaaa aaactgaagg
    28501 tcaacaggcg cgattttaat aaccacattg cgcctgtact aggaaatgtc attgtcgaac
    28561 agacaacaaa agctcactgg ctgagcctgt ttgattcgat gggacgccgt gtggtcactg
    28621 ggcagatgct gggcttaatg caacgaactt tcaggttttg ttcaaacagg ggggtgataa
    28681 gcattaatcc gcttgaaagc ctgaggcgtt cagatgtggg catggctgcg gcaaccagag
    28741 acaggaaact gagcgatgaa gagatcaaga ttgtctggtc ggctttgtct gatatggggg
    28801 aaaggcagaa gctggttgtt aaatttcttc ttatgacggg gtgcagaacc gcagagatta
    28861 gaacagcaag atgggaatgg ttcgatttta acgagaatac atggactgtt cctgcaagcg
    28921 aatataaaac gggtaagacc gtcagaagag cgttgcctcc ggcgatcgtg aaactgatgc
    28981 aggaagtgaa gaaaacctcc gtttctcagc atgttcttac gcgggcaaga tataacggcc
    29041 ctgaagatga taagccaccg caacaacctc atatcgcttt gttttctgct cagatcatcg
    29101 ctaaaacggg gatgaaaaca tggtcccttc atgacttgag aagaacggtg gcaacacgat
    29161 tatccgagtt aggtgcgcca ccacatgtgg ttgaaaagtt attagggcat catatgtccg
    29221 gcgtaatggc tcgttataat ctgcatgatt atctcgaaga tcaaaggcat tggttgcagg
    29281 tgtggcagaa ttatcttgaa gggttagttg atgcgccatt aacctgagca ttatttcttt
    29341 cccactccag aatatcagaa agccaccaac gattggggtt tccctttatt gttggtttag
    29401 gaaatggttt gctgagggac tttggctttc tgtctggagt actccagaaa tacagagtgc
    29461 ttcgtgaaat tttatatcgt tccagaatat ctttggttaa caagatttca tttgtttttg
    29521 taatgtcgtt cattttattc tccagttgaa ttgtttaatc atcgaaaaac cgcccgaagg
    29581 cggttgtcag ttgattgatg tgcggcgcat tttgcgaagg ctggcaatat gcttttcctt
    29641 ctcaatttct gctttaatca tgtgtagttc gttgtgatcg attcgctcaa attctgcatt
    29701 aaatgcgcta attgaagcgg ctctggttct cccatccagc cttcggaaga ttacctgcgt
    29761 cagagtgatt ttgcagactt caaccggata gttgttggca tcgacgaaag actgcccgcg
    29821 ctggattaga acgaacaccg gtagtattcc ttaattgtca tatcatttgc acctcgttgc
    29881 tactggctat cgccattgct ccccaaatac aaaaccaatt tcagccagtg cctcgtccat
    29941 tttttcgatg aactctggca ccatttcgtc aaaaccagcc atgtattttt catcccgttc
    30001 gaccacgaca taatgcagtc cttcacgctt catacgcggg tcatagttgg caaagtacca
    30061 ggcatctttt cgtgtcaccc acatgctgta ctgcacctgg gccatgtaag ccgactttat
    30121 ggactcgaaa ccacctagcc ggaacttcat gaaatcccgg gaggtaaacg ggcatttcag
    30181 ctcaaggccg ttgccatcac tgcataaacc atcgggagag caggcggtac gcatactttc
    30241 gtcgcgatag atgatcgggg attcagtaac attcacgccg gaagtgaact caaacagggt
    30301 tctggcgtcg ttctcgtact gttttcccca ggccagcgcc ttagcgttaa cttccggagc
    30361 cacaccggtg caaacctcgg caagcagggt gtggaagtag gacattttca tgtcaggcca
    30421 tttctttccg gagcggggtt ttgctatcac gttgtgaact tctgaagctg tgatgacgcc
    30481 gagccgtaat ttgtgccacg catcatcccc ctgcacgata gctctcacgt cgatcccggt
    30541 acgctgcagg ataatgtccg atgtcatatt gccaccttct gctctgcggc tttctgtttc
    30601 aggaatccaa gagctttcac tgcttcgtcc tgtttcagtt ctgacaatgc acgaatgtcg
    30661 cggcgaaata tctgggaaca gagcggcaat aagtcgtcat cccatgtttt atccagggcg
    30721 atcagcagag tgttaatctc ctgcatggtt tcatcgttaa ccggagtgat gtcgcgttcc
    30781 ggctgatgtt ctgcagtgtg tgcggtattt tcgacaatgc gctcggcttc atccttgtca
    30841 tagatacccg caaatccgaa ggccagccgg gcacactgaa tcatggcttt atgccgtaac
    30901 atccgtttgg gatgcgactg ccacggcccc gtaatttctt tgccttctcg ggttttgaat
    30961 ggtgcgcggc gacattcatc catccactcg gtaacgcaga tcgggtgatt gcgatctttg
    31021 cggtaaatcc ggcatgtaca ggactcattg tcctgctcaa agtccatgcc atcaaactgc
    31081 tggttttcat tgataatgcg ggaccagcca tcaacgccaa ccaccggaac gatgccgttc
    31141 tgcttgtcag ggaaggcgta aatttctttc gtccagggat taaggccgta ctggttggcg
    31201 acgatcaaca atgcgataaa ttgcgcatcg ctggcatcac ctttaaatgc cgtctggcga
    31261 agagtggtga ttagttcctg tgggtcgaca gaatccatgc cgacacgttc agccagcttc
    31321 cctgccagcg ttgcgagtgc tgtactcatc cgttttatac ctctgaatca atattaattt
    31381 ggtgacgggc gatggtttca gccatgtagc ggatgtgttc tgccatgcgt tcctgaaaat
    31441 cgacatcgtc atcaaatgca cgggaaatag cttttttgct ggccccgtga cgttgcagat
    31501 tatcgatgca tagcgattca aacaggtgtt ggggcagacc tttttccagg tcgtctgcca
    31561 gctcagcttc agtttcttca cgggcaattt gctggtagtg tcgtgcccat gactgctctt
    31621 caatgcgatc ggggataagc caggcattca tgatttatca cctccgaaat tttcaagcct
    31681 gttggcaatc atgatggcga tatcagggat tgctggcgct gtggctatac atgcggggtt
    31741 ggcgcacaaa ccatagacgg cggcaatcac gagctgtctt ttccagtcga gagttactgg
    31801 ctcagaattg gcgtcatcgc cggacgtatc actgcctggc tcgttctgaa caacggtttc
    31861 gccctcctga gcggcatcaa cagagttttc ctgaatgatc ttctcctcag tttgtgctga
    31921 gtcttctcca tcagcggcgt cattttctcc aaaagtttct gtgtaagtgc tatcgcccat
    31981 taccgcacta cagtcagggc agttaccccc gccagtctga ccacagacgc ggcagttttt
    32041 ttccagctcc ggttgcgcta ctggggctgg ctgttgctct tctggcccgt tttgttgcgt
    32101 atccgggctg ttttgtcccg cttctggggc aatttgttcc actttggact gattctggtc
    32161 ctcagtgtcg cgagcctgga tccccttaat ccacttcggg tcggttgggt cgctgatgcc
    32221 ttcaacgaat tctcctcgag aagctgccag taatttgtcg gcatcgactg gattcctggg
    32281 gggaatgttt tccctggctt tattgagttc ctcccgcagc ttctggtatt tcgtttctac
    32341 agatgagaca ttttccagtg attgcgtgtc ctcattatgt ttaactggaa ttttttccac
    32401 tgattcaggc gctgcctgtt cattagccat tgtgtctgat gcttgctgct tttcttcatc
    32461 gttatgtttc tcttctgttg ttccgcgctg cggcatcggg gctgatgagc gaccgcaggc
    32521 aatttccacg atctccggat ccgggttagt gtgatcggtt tcggttaaca ctttgttgag
    32581 atactcagtc acgcgtgccg ggatggcctc aatgccgatt ggtgcttctt tcacggaagc
    32641 caccacaatg gcgcgggaat aatccagccc accgggcatg gcgataaatt tgtcgcgaaa
    32701 aacagaaaag ggcggcttat tctctgacac gatttcttca acgcgttttg cgtgtgctgg
    32761 gtgcaggtta taaatatcca catccattga acgggccaga acgccggtgg ctacatctcg
    32821 tgcgagtgat gtcttatcat gtttgaatcc ttcaccacga tcggtaagat ttccgccgcc
    32881 agcgttagca ccggaaggcg tacgggtaat gcctgaaaca taatttccgt tctgccattc
    32941 ttttgtcagc aggccctgat caaggtagtc agttttcatc caggtggaaa tgaacttgtc
    33001 gaattcagcc gggctgatgc gatgatttgc agagtggggg aatgctttcc ctacagattc
    33061 agccaggcga ctaaggtgat ggttcgtcag tttatccagt tcatgatgcg cggcgcgcac
    33121 agcagtaagc aggctctgaa ggtaactgtc ctctgtgtcc atctccatac ggatcacgtt
    33181 attgcgttgt tctggtgtgg catgatgccg gtattttcca tcttcatctt tgctgaataa
    33241 gaagaggtga aggaagcgat gagtaaggct cagagtggcg acgggaattt cacactcaga
    33301 acagtcatcg tcgctgtccg gggattcgct tttctccaca tcatccggaa tagttccgtc
    33361 caggttatcg ttgtcatcgc cagcagttgt ggcatcttca ccgttgatgt tgtcatcgaa
    33421 ggggatagcc atcatggtga tgccatcgtt accgcctttt tcatagcggt tgcagaattc
    33481 agtatcaaac acgccttccg gtggaaggtc attcacgacg gggaaattta cgcgaacggg
    33541 ttttttgaaa tcatcctcgt cgtagcctgc atcgtccatg gctgcaatgc agcgggaaac
    33601 tgcgacagaa agttttctgg cctcggtcca gaaaaaaccg cctttgatgc caaggcgttt
    33661 tctgacttta tcgttttttg cttcgcagtg tagtgcaaaa gtctgtttat cagcgctcat
    33721 tgtatttaaa cctctggctg gattagaata agcgaccctt tgtctgattt ctccgaatac
    33781 agtgacgcag gaggaaatcc ggtagcctgc gctgccggat ttttatttca gtgaaaggtt
    33841 gcctggtttg ctgttttgtg agttgttttt tccttttcgt gctggcattc agggcagtcg
    33901 cactcagaat tttctcttgc aaactcaaga gcctgcgcca gtgtatggat ttgttgtgct
    33961 ccaatgttgc ttctgacgac ttcacgtgca gcatgaatat atgggcttgg tgttttgcca
    34021 tttaacccac ataaaacaaa acgactgtcc ttatattcgt tatttccaac ctctgtaagc
    34081 actgaaaaag aaaatacaaa gtcgatttgg tattctttgc atattttact gatgcgttct
    34141 gcaatttctt tgagttcatt tattgcatca gggctggttt cggaaaatat ttcattttgc
    34201 gcaagttctt tcattttgat ttttccgtga ttgataatta atgcggtttt tatttcggct
    34261 tgtctgttac agatatggaa ggtgtatttt aattactcgt cacaacgact ctgcttttac
    34321 gggtaagcca tcgcgcccga tgaatacttt aatcatgcag ttggtaatgc atgtttttgt
    34381 agtcaggcta cgaatataga gctttctctt ttcaatattg tttgctgaag cgatatatgt
    34441 gcggccttca tgaagaacat aatcgccagg tgtcacgcac tgacgtggta tttcatcagt
    34501 tccgaagtga tgagcaatca taattatctc cattttcaca aatgaacttt gttgatgcgg
    34561 tgcctggtgc ctccaggtga cgttaaccag ttaataatta acgccggagc agggggcgat
    34621 gattttccgt gacatcctgt cggtttaact gttccgcgtg cgctgagccg cattcaccgc
    34681 atcacaagat tcactttaaa aagggcggac atcagccagc aattaaactg atgccgccaa
    34741 tgactacaca cagcaatgtt gttattcaca accggaagtg cacggtcgaa gaaatctaac
    34801 gacaagcctt caaagggaaa gagtcttcgc cgtgcgtttt cgtgttgtac cctggctttt
    34861 cagggtaata tctgttcagt aaactgacag tgccggatgc tcacccgtgt ccggcgcacg
    34921 aactccacct gacccgtgga gaactcctca actacaaacc ccgtaaggag agtgaattta
    34981 tgacacaaga agaaaaagtg atgtttctga tgcggctggc tgtcgatacc tacaacacac
    35041 aattcaggga gaaagatata cctcaaaagg cagttcctgc cgcggtagat aagggcggtg
    35101 ctattgccgt attttacgat gcatttgaat cattttttga tgaaaaactc gacgctgtta
    35161 gcgacttcgg aacatcgagt aataaataac gttcattacg gttcttaaac aacaatcagt
    35221 gggcttgatg ttgttctttt caagctcact tgccatcact tccattattc ttgcacttac
    35281 acgaattatt tggtggctgt aagcgacgca actgtcgctg atattgctgt ttatttctat
    35341 tacttcattt tcactggcgg cgctaaattt ggatatgccg tttccattgt cctgtttcag
    35401 tgctgtttcc gccatccgga tgcgttcctg cgttgcggaa tttgggctaa gtcgataaac
    35461 ctgtctggca tcttccagaa gcatggcgat aatatgcttc agttctgttt cgttcatagt
    35521 taactccggt agttgcaatt tattaatatc aggcggccag ctctttaagc ttctgaactg
    35581 ctttattcat ttcatccata cagtcgatga attcgtccag tttaagctgc atttttccgg
    35641 cggcctgaag aatttcaagt tttaagggcg caagtttttt gttgaataca tcatcttcct
    35701 ggcgtttttt ctccgcaatt tcggcggctt tcagcaattc ttcagcctgt tttctgagtg
    35761 tttctggtga aggtgatact gctttgaggt tattcatttc aggttcctgt tgttgcagaa
    35821 ttaatttgtt gcattctctg cgtgagcgtt tcattgcaga ttcgcacttc ttgcaataaa
    35881 tggctctccc gtcacgcttg ctggcatctg aataaaaatc attgactgat tttgtctgcc
    35941 cgcatttggt acataatttt gattgaaggt ccttaatgat tgtgacgttt ccttcccgtt
    36001 tgggatatcc atattttttg tggcggaaaa aggttactcc gttaccagaa ccttcttttc
    36061 tgaccttaaa tacaatgatg ctttcccgtt tatcgataaa atcgatgagc tggtttctct
    36121 cttttcctga cagttcggca aacgtcttaa ctttttttgt aacctccgta aggcgaacac
    36181 catcaggcat tttttcaacg aagtttttaa tctctgcaag cggacgccat aatttttgcg
    36241 gcaaaactga aggcgtggtc atcaatcacc tcgctgttat tgttcctgat ttgcgatagc
    36301 cagctgcgta aagtgcagtg tttggaagac aagtagagcc ttcatatttt ctggttaatg
    36361 tcgttatcgt gactacttca gctcgcattg cggctttgcg tttgcactgt aagtacaccc
    36421 gtgaaggtgt ggggcggtgc agtttatcag cgcttgatgc ctccagaccc tgaagctcgg
    36481 ccctgcgtgc tctgcggcgg gaagctgcgg tgcctttaaa ttctgttttt ctggacatag
    36541 atttctcccg aataaacttt ggcgatgcaa tctcgaagcc tcccctgaga tgattgcttc
    36601 ggcattgcac ccgacagctt atgcggttgg gcgatctggc tttgcagcca cgtagtcgag
    36661 tgttcgacgt tgtttaaaga gctttccaat tggggccgcg ttgttatcgg tgacctgttg
    36721 gtgaaatgat tattaactgt tggttagcat tagtcaataa ccaaaagtta aaattattgt
    36781 ggggaaattc tatgtttttg atttttagtt aattattatt gcttgttcgg tgagcgacgg
    36841 ttatcaagta tgaaggggta tagaagctag gatagatgag ttttggctag ctgggttaat
    36901 aaaaaacccg gctctgaggc cggggggaga ggggctaaag cagaactgaa taccagaaaa
    36961 cttttccgat aatctccacg ctttgagtat ctgcttcttc gtcagggtac tcttcactgt
    37021 tataactgcg tatgattagt tttccaccgg ggcgtctgta caatagtttt atacgcttca
    37081 gtttttcatc accgacacca ggctgtgcga tggcgtagag tttgccgtca acaatacgtt
    37141 tgttgtttgt gtctactgcg attgttgttc catctggtat cacgggctcc atgctatcac
    37201 cagtagccgg aaaacaaagc acaccagaac catcactgtt agctccgaca cggcgcaggg
    37261 ttgcttttga gaagcgtaat ttaaaaccat tatgatcttc gcaatgaact cttccgtcac
    37321 cgcatgcaaa ctcaatgtct ttcagatatg gcacctcaac ttcatcatct cgtagtggtg
    37381 tatttctgtc ccaagtttca agggaacccc attctgattc gtttggaatt gaaggtcgct
    37441 gttttacgga tggctcgtta ctgccatact ccaaccactc cggcctcacg gaaagccagt
    37501 cactaagtgc caaaatactt gttttttctg gaattgatgc agaattaagc catttccaga
    37561 tccccggttc agagatatca atgcctcgca gtttcattgc attctgaatc cgcttagcct
    37621 gaccgcgtcc gcccacacct gcatctaata atgcctgtag tagtctcttt gagaacgctt
    37681 cttttagttc atctttttta accatacgtt aattatcaaa catagttgac ataactgtca
    37741 gctaatagtt aacattaact cgaagttaac taaattaatg gatagcagta tgaatccgat
    37801 acaattcgca gtaaaagctg ttggtggtca aacagtcgct gcccgtttat gcgggttgtc
    37861 aaatgttgca attcataagt gggttaaaaa cggagcgtta cctcgcaccg aatacacgat
    37921 gaaaaccaac tattcggaaa ttctcgccaa tgcatctgat ggcaaattta ccgccgaatg
    37981 gttacgcaat gtagcgaatc ctgatcgtca acgataagct caattaacac tgctcttttc
    38041 acaatggaca ttcgtcctac gtcgctgaaa agcgaggccc aagatatctg accaattaag
    38101 gccatatgcg tttccacgca tacctttcaa ctaactattc actatttgga atcataagaa
    38161 atgtcacaaa caagttacag caaactgtcg cagcgcgata tcgatcgcgc tgaaacggat
    38221 ttacttatca acctgtcagc tctgacgcaa aggggactgg cgaagatgat tggctgccat
    38281 gaatcgaagg tcagtcgtac cgactggcga tacatcgcgg cgattttatg cgcgttccag
    38341 atggcatctg atatcagtcc gatcagccgg gctttccagc atgccattaa cgttcatgca
    38401 aataaaaaac gtccggttgg ggccggacgt tctgagcaaa tcctgatgaa tatctgatat
    38461 tcaggcaggg catggagcaa tacacggtaa taattctgcc acatctggaa gaatttcgcc
    38521 agcaacaaca ccaaccgcag cagcctgaag ccgattgggt taacccggag ataccgggac
    38581 cgtctgtgaa gatgtgcagt cacaccaatg tgcagtcaca ccaaccgcag cagcctgaag
    38641 ccgattgggt taacccggga gataccggga ccgtctgcgg tatggagtaa atcttgtatg
    38701 cgaggggact atgcgtaatt acgcaacaat ttcacctcag ttctggttag gcgatacagg
    38761 gcgaaaacta aggaagtctg gtccggaatg tatggtagtg gcgttgtata tgatgacctc
    38821 gcctcattcc aatatgctgg gcctttatta cctgcctgtt ttgtacattg ctcacgaaac
    38881 cggacttgat cctgaagggg cttctaaggg gcttcaaatg gcttgcgagg ctggtttttg
    38941 cagctatgac catgattctg aggttgtatg ggtgcatgaa atggcagcat ggcaggttgg
    39001 tgaagcgctg aaaccaggcg ataaccgttg tgctggggta agaaatgaat attccgcgtt
    39061 gctggaaaat ccttttttat catcctttta tgatagatat aaggatgatt tccacctgga
    39121 tgtcagacgt gaatcatgtc ggaaaattga agccccttca gagccccttt caagccaaga
    39181 acaggaacag gaacaggaac aggaaagaga taaaaccctt ctggtccatg gcgaaaaaat
    39241 cgccacggac ccactggtgg attcttgtcc cgttctgact gaacgtccag gaccagctgg
    39301 catgacaccg gaagcagatt ccgggcgttg tgtgcagcag gtgctgctcg tcgaaccgga
    39361 gcaacaacac caaccgcagc agcctgaagc cgattccgcg atgagcggga agccgattgg
    39421 gttaacccgg gcgatgccgg gaccgtctgc gggacgagtt gattatcctg acgtgttcga
    39481 acgggtctgg cgtgaatatc cgcatcgggc agggtcaaac ccgaagaaat ccgcgttcaa
    39541 tgcctggagg gccagattac gcgaaggggt gtcaccggat gtcgtgctgg atggcgtgag
    39601 gcgttacgca agatacctgg aggctaccgg gaaagcggga actgaatttg ttcagcaggc
    39661 atcgacgttt tttggcccga acaggaattt cgaaaatccg tggtcgctgc cgaaggctgg
    39721 cgcagtcagc cagcgttgcg tgaaccacat ttctgaaccg gacaccgaaa ttccgccggg
    39781 gttcaggggg taatcagcca tgaaaaacat ttcgacagga gggattcttg aacgggtgcg
    39841 ccgtctggca ccaccgcacg tggcagcacc gttccggacg accgacgaat ggcgggaatg
    39901 gcaactggct gagggccgta agcgcagcga ggaaattaac cgccagaatc atcaggcgcg
    39961 ggttgaaaaa atcctgaacc gtgcgggcat ccagccgctt cacaggaagt gctcattcga
    40021 gaactaccgg gtgcagaacg acggtcagcg ccatgctctg agccaggcga aatccattgc
    40081 cgatgaattg atgaccgggt gtacaaactt cgtgttcagc ggtaaacctg gtaccggtaa
    40141 aaatcacctg gcagcagcga ttggcaaccg gctgatggcg aaggggagaa gcgtgattat
    40201 cgtcaccgtg tccgatgtca tgagcgtgtt gcatgacggc tatgacaacg gcaagtccgg
    40261 tgagaaattt ttacaggaac tttgcggggt cgaccttctg gtcctggatg aaattggcat
    40321 gcagcgggat acgcgcaacg agcaggtcat actgaaccag atcgtcgacc gcagaacggc
    40381 atcactgcgc actgtcggaa tcctgacgaa cctgaatcac gcagcgatga gcacactcct
    40441 cggcgagcgg gtgatggacc gtatgaccat gaacggtggt cgttgggtga gttttaactg
    40501 ggagagctgg cgatcaaacg ttgggcgtca gggtatgtga gaatttttga cgaggtaaat
    40561 tttcgatgga aaccgtactg catgcactga aagcgatggg aaaagccaat tctgttgaac
    40621 tggcggcgcg gcttgatatc agccgtgaag aagttctcaa cgaactgtgg gaactcaaaa
    40681 aaaatggcgt tgttgataaa acgggtcaca cctggtttct ggctgtcgaa ggtgaatccg
    40741 gggtaaccga agggcaggca ctacaacctg aagcgccgga tgtggtaacc gaagaggtcg
    40801 ctccaaaagt tagcgctgac atgatgattg agtttatctc tcaggagggg gctaaaacat
    40861 gtgaggaact ggcgggtaag ttcggtgtca gcactcgcaa ggttgcttcc acgctggcgg
    40921 tggtaaccgc aacgggacgc ctggcacgcg taaatcagaa cggtaaattt cgctactgca
    40981 tgcctggaga taatttacca gcagagccga aagctgcatc ggtagcagaa accgatggta
    41041 aagcctttcc tcagccagca ggtgttgcgt taccggtaca ggaagctgca acacaggaag
    41101 atattaaaac agaaactgtg gcggacattg tgcagtcgtt gccatcgttt actgaaacgc
    41161 gagcggatga cctggtttta ccatcgctgc atatggcaaa ccgcgaactg cgtcgggcga
    41221 aaaatcatgt ccagaagtgg gagcgtgtct gcgccgcgct gcgggagctg aacaagcacc
    41281 gggatattgt tcgacagatt gctgattctt cccgccgtgt tgcatcggaa aagtgattgc
    41341 cggaggcgct tatggcaaaa gtatttacac aagaagagcg ggaaaaaatt aaggtacagg
    41401 ttgttgaact tgtgcgtctg agcgggcgcg agacgttacg gcaactggaa gcgaaaacag
    41461 gtgcgacaag atatctgatg agtgttcttg ccagagagct ggttgccagt ggcgatttat
    41521 acaactccgg ctacgggcta tttccgtctg aacaggcacg taaggactgg ataaaggccc
    41581 gcaaaaagat gtcgaaagca gcagtgaaaa aaaagagcga cccggacctg gtttattcat
    41641 taccagacgg agaaatacgt cgttacgaca ggcgtctgaa cataatctgt cgcgagtgcc
    41701 ggaagagtga ggttatgcag cgcgtgttgg cgttttatca ggggaaattt caggaggtga
    41761 tgctgtgagt acacctgcaa ccattcttga tatgtgctgt ggcagccgta tgttctggtt
    41821 cgataagaat gacgaccggg cgatatttag cgatatcaga aaggaagagc atacattgtg
    41881 cgatgggcga cgcctaatta ttagccctga cctgatagca gattttcgtg cattaccatt
    41941 tgcagacgca tcgtttccgg ttgttgtatt cgaccctccg catcttgagc gtgttggtga
    42001 taacgcctgg atgggaaaga aatatggacg gctgaataaa gatacctggc gtgatgattt
    42061 gcggcaggga tttaaagaag cctttcgtgt gttgtggcca cacggtgttc tgatttttaa
    42121 atggaatgaa acgcaaatac ctgtccgcca gatattggca ctaaccgacg cgaaaccaat
    42181 catcagccag cgcaccggta agaacgataa aacacattgg attatttttg tgaaggaagc
    42241 gaccagtggg taaatcaaaa tgccaggttt atggcaacaa gatagaaccg tgtacggcac
    42301 tggcaaactc ccttgagcat gatgctgaat acacgacgcg aaaaggtctg ctgatataca
    42361 aaatctggaa tgagaattta actcgcgacc ctgatttagt gatgttgcgt tccggtgaat
    42421 tttctaaatc accagttcgg gtttcatttt gcccgttctg cggtgaaagt ctgaaaacat
    42481 gggagaacag aaatgaatga aattagagaa ataccagtag aacgtgatga atatggctgc
    42541 tggacacatc cagaatatga aaagttctgt gacggtcggg aatatatttc aacggaagag
    42601 tttagcgcct ggatgaaggc aaataatctt cagtggacta tttgcagcat ggatgaagat
    42661 tatttaaatc cggttgcaga tgaccccgat atttccacct gggaaccgga acgaccagag
    42721 ggcgaaggct ggtttattgg ctccattcat gatactgaag atggtccggt ttgtgtctgg
    42781 ttgcgaaaca aggtgatggc atagatgaga atagaaaaag cgaaagcaca gctcaggata
    42841 atgcttgctg gtccggctgc gagttatacg gcacactcac cagccataaa gaaggttctt
    42901 gatgaactgg aagccaaaga caagcgcatt gcagaactgg aagcaaggga agtccagcta
    42961 ccggctggct atgaacttcg atatggacac ccgataaatg ctgatgagcg tcatgtcatg
    43021 atacctaaag aaaatggtag ctggctttat cttgctgatt tagaacatgc actacgtgtt
    43081 gcagggattc gtattaaagg agactaacgt ggaaataaaa ccagaagatg agttaagtaa
    43141 tattgtttta tttccggtaa aagaggatga cccacgtaat caggttaatt ttctttatga
    43201 gccatcggaa agaccatatt gtcatcacga ctctgtccgg gttgacgaaa aagagcgtca
    43261 ggtccgctgt aaaatctgcg gtgcagttgt ggagccgttt gactggatgc tctctgtggc
    43321 gaaaagagaa accagactgg cagatgatgt aaggctattg cgtcaggagg aacgggaaag
    43381 gcggaaaaat atagaaaagc taattcagat tgagcgtaac gcaaaagcgc ggatgcgcag
    43441 agcgacaaaa tccagaactg aataattaaa tttagcactg ttaaaaattt aatccttaac
    43501 cggagggatt tctgtaccct cagaacatca ggagtccgcc tgaaagggca gtaatgaaaa
    43561 atgactgaat taacaaaaga gcaattaatc gaagaagcta aattaaaaat agcgattgcg
    43621 aaatgccacc ctaattcaga gatggcacgg gtagagggtg agttattcaa aattgcactg
    43681 gcatcgctgg tagcagagcc agctggtaaa ttgcatgaat acaaaccagt gggatatcag
    43741 cgtctggtcg acgagttaac catgctggta aagcagttag cctggcaact gaggaaagcg
    43801 aaaccgggct gcaaattacc ggataaagcg atggactatc tggagcgaaa cggacttata
    43861 agcgtggagg atgttttacg atgacctggc ctgaggcgtt cacaacggca ggaattgcac
    43921 tggcggtggc gctggtggag tattcgattt gccgctgggg ttgacgtaat tacttatccg
    43981 ggattatatt cttcgcacgg tcgagttggc cgtcgggatt ggaaccccgg atagagaccg
    44041 cgacaaaaac acgccgcgag cgtgtttttt attgtcgtat gcacgcgcac atctgaatta
    44101 tggtggggcg tataggggag ctgaaaagct cgccggttgg tttcccggta gttcgaaccc
    44161 tgtacgtctc accacccgat gattcgaacc tgacggtggt gatagttaac ctgatgagct
    44221 gaaagtatta ctacacaaat cactacagga ataatccccc accgaaataa ttcaggcaac
    44281 agcctgaatg tagtaggaat ccacgacctt tagggcgtgg aggatgtcaa agagtttagg
    44341 gcgcactgga taaataaaat aaaactgtta acgatattta cctccagcac tcaatataaa
    44401 taactgaaat gaagacccca atacaaaatg cgttgacatg accgaacatt aatatccata
    44461 catcaacttc tggtatatgt ttagcgat
//
LOCUS       DAZKHK010000024        44336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKHK010000024 DAZKHK010000000
VERSION     DAZKHK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.024"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(123..209)
                     /locus_tag="ACHOS5_001368"
                     /pseudo
     CDS             complement(123..209)
                     /locus_tag="ACHOS5_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            251..481
                     /locus_tag="ACHOS5_001369"
                     /pseudo
     CDS             251..481
                     /locus_tag="ACHOS5_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            533..1468
                     /gene="ttcA"
                     /locus_tag="ACHOS5_001370"
     CDS             533..1468
                     /gene="ttcA"
                     /locus_tag="ACHOS5_001370"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP9776206.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1597..2970)
                     /gene="dbpA"
                     /locus_tag="ACHOS5_001371"
     CDS             complement(1597..2970)
                     /gene="dbpA"
                     /locus_tag="ACHOS5_001371"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP9776207.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVICFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3000..3173)
                     /gene="ynaL"
                     /locus_tag="ACHOS5_001372"
     CDS             complement(3000..3173)
                     /gene="ynaL"
                     /locus_tag="ACHOS5_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP9776208.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3448..4431)
                     /gene="zntB"
                     /locus_tag="ACHOS5_001373"
     CDS             complement(3448..4431)
                     /gene="zntB"
                     /locus_tag="ACHOS5_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP9776209.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4445..4492)
                     /gene="ydaN"
                     /locus_tag="ACHOS5_001374"
     CDS             complement(4445..4492)
                     /gene="ydaN"
                     /locus_tag="ACHOS5_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP9776210.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4686..5918
                     /gene="dgcM"
                     /locus_tag="ACHOS5_001375"
     CDS             4686..5918
                     /gene="dgcM"
                     /locus_tag="ACHOS5_001375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP9776211.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5939..6502)
                     /gene="smrA"
                     /locus_tag="ACHOS5_001376"
     CDS             complement(5939..6502)
                     /gene="smrA"
                     /locus_tag="ACHOS5_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP9776212.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRRMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6697..8211)
                     /locus_tag="ACHOS5_001377"
     CDS             complement(6697..8211)
                     /locus_tag="ACHOS5_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFP9776213.1"
                     /translation="MMSMRKHIAFASMCIGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMIFF
                     RALQGVAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWITDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLKISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQVRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLQWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SGSDQTSEILASTKLLSQLMLREAQTMAFSDTFLLISGLLFIAFLLVPAMNKSS"
     gene            complement(8208..9235)
                     /locus_tag="ACHOS5_001378"
                     /pseudo
     CDS             complement(8208..9235)
                     /locus_tag="ACHOS5_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            9332..10147
                     /locus_tag="ACHOS5_001379"
     CDS             9332..10147
                     /locus_tag="ACHOS5_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9776214.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            10496..10648
                     /locus_tag="ACHOS5_001380"
                     /pseudo
     CDS             10496..10648
                     /locus_tag="ACHOS5_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            10659..11174
                     /gene="ogt"
                     /locus_tag="ACHOS5_001381"
     CDS             10659..11174
                     /gene="ogt"
                     /locus_tag="ACHOS5_001381"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9776215.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNLGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            11369..12121
                     /gene="fnr"
                     /locus_tag="ACHOS5_001382"
     CDS             11369..12121
                     /gene="fnr"
                     /locus_tag="ACHOS5_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP9776216.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            12273..13223
                     /gene="uspE"
                     /locus_tag="ACHOS5_001383"
     CDS             12273..13223
                     /gene="uspE"
                     /locus_tag="ACHOS5_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP9776217.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(13273..13530)
                     /gene="ynaJ"
                     /locus_tag="ACHOS5_001384"
     CDS             complement(13273..13530)
                     /gene="ynaJ"
                     /locus_tag="ACHOS5_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP9776218.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            13774..14805
                     /gene="ynaI"
                     /locus_tag="ACHOS5_001385"
     CDS             13774..14805
                     /gene="ynaI"
                     /locus_tag="ACHOS5_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP9776219.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVTEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            complement(14856..16469)
                     /gene="mppA"
                     /locus_tag="ACHOS5_001386"
     CDS             complement(14856..16469)
                     /gene="mppA"
                     /locus_tag="ACHOS5_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP9776220.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKAAPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRFLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(16806..17705)
                     /gene="pgrR"
                     /locus_tag="ACHOS5_001387"
     CDS             complement(16806..17705)
                     /gene="pgrR"
                     /locus_tag="ACHOS5_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP9776221.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSATTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKGKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            17843..18763
                     /gene="ycjY"
                     /locus_tag="ACHOS5_001388"
     CDS             17843..18763
                     /gene="ycjY"
                     /locus_tag="ACHOS5_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9776222.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDEPLQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGESRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGSNHMDLYDGKVYMAEAISVLAPFFEETL"
     gene            18834..19472
                     /locus_tag="ACHOS5_001389"
     CDS             18834..19472
                     /locus_tag="ACHOS5_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9776223.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITGIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            19763..20491
                     /gene="mpaA"
                     /locus_tag="ACHOS5_001390"
     CDS             19763..20491
                     /gene="mpaA"
                     /locus_tag="ACHOS5_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP9776224.1"
                     /translation="MTVTRLRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(20466..21431)
                     /gene="ycjG"
                     /locus_tag="ACHOS5_001391"
     CDS             complement(20466..21431)
                     /gene="ycjG"
                     /locus_tag="ACHOS5_001391"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP9776225.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            21550..22056
                     /gene="tpx"
                     /locus_tag="ACHOS5_001392"
     CDS             21550..22056
                     /gene="tpx"
                     /locus_tag="ACHOS5_001392"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP9776226.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(22100..23641)
                     /gene="tyrR"
                     /locus_tag="ACHOS5_001393"
     CDS             complement(22100..23641)
                     /gene="tyrR"
                     /locus_tag="ACHOS5_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP9776227.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(23789..24850)
                     /gene="ycjF"
                     /locus_tag="ACHOS5_001394"
     CDS             complement(23789..24850)
                     /gene="ycjF"
                     /locus_tag="ACHOS5_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP9776228.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(24847..26244)
                     /gene="ycjX"
                     /locus_tag="ACHOS5_001395"
     CDS             complement(24847..26244)
                     /gene="ycjX"
                     /locus_tag="ACHOS5_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP9776229.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYFSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            26401..27399
                     /gene="ycjW"
                     /locus_tag="ACHOS5_001396"
     CDS             26401..27399
                     /gene="ycjW"
                     /locus_tag="ACHOS5_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9776230.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTDALIESGHKNIACLHAPLDVHVSVDRVNGYRQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            complement(27450..28355)
                     /gene="ompG"
                     /locus_tag="ACHOS5_001397"
     CDS             complement(27450..28355)
                     /gene="ompG"
                     /locus_tag="ACHOS5_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP9776231.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETLALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(28400..29482)
                     /locus_tag="ACHOS5_001398"
     CDS             complement(28400..29482)
                     /locus_tag="ACHOS5_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9776232.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            complement(29496..30155)
                     /gene="pgmB"
                     /locus_tag="ACHOS5_001399"
     CDS             complement(29496..30155)
                     /gene="pgmB"
                     /locus_tag="ACHOS5_001399"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9776233.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(30152..32419)
                     /gene="ycjT"
                     /locus_tag="ACHOS5_001400"
     CDS             complement(30152..32419)
                     /gene="ycjT"
                     /locus_tag="ACHOS5_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFP9776234.1"
                     /translation="MTRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKNDINELVNLPDVIGMEITLNGELFALSRETWQRELDFASGELR
                     RNVVWSSASGARYAIASRRFVSAEQLPLMALEISITPLDADAPVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLPAGKTLSLQKRVWIDWRDDRHVALDEWGSASLRQLEMCVQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSEPTIARSLLRYRWHNLTGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELRLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGNF
                     ILPFNGTATTHQEGE"
     gene            complement(32416..33471)
                     /gene="ycjS"
                     /locus_tag="ACHOS5_001401"
     CDS             complement(32416..33471)
                     /gene="ycjS"
                     /locus_tag="ACHOS5_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9776235.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTVEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(33481..34269)
                     /gene="ycjR"
                     /locus_tag="ACHOS5_001402"
     CDS             complement(33481..34269)
                     /gene="ycjR"
                     /locus_tag="ACHOS5_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9776236.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAENPAQAYRDSLAWLRTC"
     gene            complement(34288..35340)
                     /gene="ycjQ"
                     /locus_tag="ACHOS5_001403"
     CDS             complement(34288..35340)
                     /gene="ycjQ"
                     /locus_tag="ACHOS5_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9776237.1"
                     /translation="MKKLVATAPRVAALVEYEDRAISANEVKIRVRFGAPKHGTEVVD
                     FRAASPFINDDFNGEWQMFTPRPANAPRGIEFGKFQLGNMVVGDIIECGSEVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAICYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPITRRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(35371..36213)
                     /gene="ycjP"
                     /locus_tag="ACHOS5_001404"
     CDS             complement(35371..36213)
                     /gene="ycjP"
                     /locus_tag="ACHOS5_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9776238.1"
                     /translation="MATNKCTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFIDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(36200..37081)
                     /gene="ycjO"
                     /locus_tag="ACHOS5_001405"
     CDS             complement(36200..37081)
                     /gene="ycjO"
                     /locus_tag="ACHOS5_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9776239.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     LLNPNIEATFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(37102..38394)
                     /gene="ycjN"
                     /locus_tag="ACHOS5_001406"
     CDS             complement(37102..38394)
                     /gene="ycjN"
                     /locus_tag="ACHOS5_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9776240.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     DDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(38408..40087)
                     /gene="ycjM"
                     /locus_tag="ACHOS5_001407"
     CDS             complement(38408..40087)
                     /gene="ycjM"
                     /locus_tag="ACHOS5_001407"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFP9776241.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSMRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSMPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLRYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            complement(40301..40615)
                     /gene="pspE"
                     /locus_tag="ACHOS5_001408"
     CDS             complement(40301..40615)
                     /gene="pspE"
                     /locus_tag="ACHOS5_001408"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP9776242.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKDILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(40690..40911)
                     /gene="pspD"
                     /locus_tag="ACHOS5_001409"
     CDS             complement(40690..40911)
                     /gene="pspD"
                     /locus_tag="ACHOS5_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP9776243.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLARRYKR"
     gene            complement(40920..41279)
                     /gene="pspC"
                     /locus_tag="ACHOS5_001410"
     CDS             complement(40920..41279)
                     /gene="pspC"
                     /locus_tag="ACHOS5_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP9776244.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(41279..41503)
                     /gene="pspB"
                     /locus_tag="ACHOS5_001411"
     CDS             complement(41279..41503)
                     /gene="pspB"
                     /locus_tag="ACHOS5_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP9776245.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(41557..42225)
                     /gene="pspA"
                     /locus_tag="ACHOS5_001412"
     CDS             complement(41557..42225)
                     /gene="pspA"
                     /locus_tag="ACHOS5_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP9776246.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            42391..43368
                     /gene="pspF"
                     /locus_tag="ACHOS5_001413"
     CDS             42391..43368
                     /gene="pspF"
                     /locus_tag="ACHOS5_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP9776247.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            43524..43661
                     /locus_tag="ACHOS5_001414"
                     /pseudo
     CDS             43524..43661
                     /locus_tag="ACHOS5_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            43795..44040
                     /gene="ymjA"
                     /locus_tag="ACHOS5_001415"
     CDS             43795..44040
                     /gene="ymjA"
                     /locus_tag="ACHOS5_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFP9776248.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
BASE COUNT        11397 a        11445 c        10385 g        11109 t
ORIGIN      
        1 tcaccgaaaa gtttcacaaa atatcccctg atagaaaact aaaaaacact gcataatatc
       61 gctattctgg cacttgcgaa aaattatatt ctgcacctgt tgtagcgttc tatttctccg
      121 cctggcaacg aatgcacaat gtccgccaca gtttctgttc taatttcttc ctgtgttacc
      181 gcatcctgta cgggtagcgc aacaccagca tgctaagcaa ggctttccca tcagatttca
      241 ctacgactat ttgggagcga gatggaatgc agcagtaaaa cgtgctggta ttcgccgccg
      301 taatccgtac catacgcgac atacttttgc ctgctggctg ttgacggcag gagcgaaccc
      361 ggcatttata gccagccaga tggggcatga aactgcgcag atggtgtatg aaatttacgg
      421 tatgtggatt gatgacatga acgacgaaca ggtagccatg ttgaatgcgc ggttatcgta
      481 gttgcaaagt ttgccctcaa tatgccccat ttagtaccag agaactgaaa taatgcaaga
      541 aaatcaacaa attacaaaga aagaacaata caacctgaac aaattacaaa aacgtctgcg
      601 tcgtaacgtg ggcgaagcca ttgctgactt caatatgatt gaagaaggcg atcgcatcat
      661 ggtttgcctc tccgggggta aagacagcta taccatgctg gagattctgc gcaatttgca
      721 gcaaagcgcg ccaatcaatt tttcgctggt ggctgttaac ctcgatcaaa agcaaccggg
      781 cttcccggaa cacgttctgc ccgagtatct tgaaacgctg ggcgttgagt acaagattgt
      841 tgaagagaac acttacggta tcgtgaaaga gaagattccg gagggcaaaa ccacttgctc
      901 actgtgttct cgtcttcgtc gcggtatcct ttatcgcacc gcaacggaac tgggggcgac
      961 gaagatcgcg ctgggtcacc atcgtgacga tatcctgcaa acgctgttct taaatatgtt
     1021 ctacggtggc aagatgaaag gtatgcctcc gaaactgatg agcgatgatg gcaaacatat
     1081 cgtgattcgt ccgctggcct actgccgcga gaaagatatc cagcgctttg ccgatgcaaa
     1141 agcgttcccg attattccgt gcaacctgtg cggttcacag cctaacctgc aacgtcaggt
     1201 gattgctgac atgttgcgtg actgggataa acgttatccg gggcgtatcg agacgatgtt
     1261 cagcgcaatg cagaatgtgg tgccgtcgca tctgtgcgat accaacctgt tcgatttcaa
     1321 aggcattacc cacggttctg aagtggttaa cgggggtgat ctggcgtttg atcgcgaaga
     1381 gatcccacta caaccagcgg gctggcagcc tgaagaagat gaaaatcagt tggatgagtt
     1441 acggctgaat gtggttgaag tgaaataacc tggatagcgc ccgatgcgca agcgtatcgg
     1501 gctactctta tggaggccgg ataagacgcg gccagcgtca catccggcaa tcccgaagaa
     1561 gatgtttact cttgcaccct gcaattcaac atttcattat tttaataacc gcacccggca
     1621 cgtttttcct ttaatcttcc cgccctgtag ctgtttccat gctttatgag caacagcctg
     1681 acgcaccgcg acataaacat gcgccggatg cacggcgatt ttgccaatat ctgcgccatc
     1741 aagcccgatg tctcctgtca gcgcacctaa tacatcaccc gggcgcattt tggctttttt
     1801 cccgccatcg atacacaacg ttgccatttc tgcttccagc ggcacaatgg aactattcgc
     1861 tggcggcgtt tgccagttaa gttttatctg caacatgtca gaaatgatat tggcccgctg
     1921 tgcttcttcc ggggcacaga aactgatcgc cagaccgcta tttcctgcac gagctgtacg
     1981 accgatgcga tgtacatgaa cttcagggtc ccacgccagc tcaaagttca ccaccagctc
     2041 aagcgatttt atatccagac cacgcgcagc aacatcagtc gcgaccagta cacgggcgct
     2101 accgttagca aaacgtacca gggtctgatc gcgatcgcgt tgctccagat cgccgtgtaa
     2161 cgacaatgca ctttgcccta cttcattcag cgcgtcgcag acagactggc aatctttttt
     2221 ggtattgcaa aacaccacgc aagaggatgg ctgatgcaag cttaataacc gttgcaacag
     2281 aggaattttg cctttgctgg atgtctcata aaattgttgt tcaataggtg gcagagcatc
     2341 tgttgagtca atttcaatcg ctaaaggatc gcgttgcact cttccgctga ttgcggcgat
     2401 ggcttccggc caggttgccg aaaacagaag cgtctgccga gatgctggcg caaaacagat
     2461 gacatcatca atggcgtcgc taaatcccat atccagcatg cggtcggcct catccatcac
     2521 cagcgtattc aacgcatcca gtgatacagt gcctttttgc aggtgatcca gcaaacggcc
     2581 cggcgttgcc acgataatat gcggcgcatg ttgtaacgaa tcacgctgca taccgaacgg
     2641 ttgaccaccg cacaacgtca aaattttggt atttggcaga aaacgcgcca gccgacgcaa
     2701 ttcacctgcc acctgatccg ccagttcacg cgtaggacac agcactaaag cctgggtttg
     2761 aaatagcgac gcatcaattt gctgtaacaa gccgaggcca aaagccgccg ttttgccgct
     2821 gccggttttc gcctgcacgc gaacatcttt tccggcaagg atcgccggaa gcgcggcggc
     2881 ctgcaccggc gtcatggtta aataacccaa ctcattaagg tttgtgagtt gggcgggagg
     2941 caaaacattc agggtagaaa aagcggtcac aatctattct cgtggtcatc gacgcaaagt
     3001 cagcaggcgc gtatcctcgc agatctacgc tcacgatgcg acaatttaat cggttcttca
     3061 tcgggtggtg ggtcaggcat gggttgcggg cgagggatcg gatcgggcac tggaacagga
     3121 tcgccaggaa tcggttcagg gacaggaatt tgcaaataaa taagtgtcgt catatttccc
     3181 tccggtcatt gggtggactc ttaaagggta gacgctgata aataacaggc aaaaaaaagc
     3241 cgactcatca aagtcggcgt cgtacgaatc aattgtgcta tgcagtaatt caaaaaagga
     3301 agtaagacaa tatggagcgc aacgcccatc gcttgacgtt gcattcacct gcaagagaga
     3361 tattgccctg aatgggtaga gagtttattg acttcgctca aactttacgg cgtttttgta
     3421 tacagacagc cggaaaaatt gcttttgtta caaccattta ctacgatgca accataaagc
     3481 aacaccacca ataagaacaa ctaacagaat acaaaaaatt gaaaatccga attgccatcc
     3541 accgccaggg atcccaccaa ggttgacgcc aaataaccct gtcagaaacg tactgggtaa
     3601 aaagaccatt gccatcaacg acattgtata ggtacgacga gctaaatttt cctgcatcac
     3661 ctgagcgatt tcatccgcca tcacgccagt ccgtgctata caggcgtcga tttcgtcaag
     3721 accgcgccca aggcgatcgg caatatcctg catccgacgg cgttggtcat cgctcatcca
     3781 cggcaaacgt tcactggcaa gacgagcata aacatcacgt tgcggtgcca tatagcgacg
     3841 catcacaatt aattgtttgc gcagcagagc caggaatcca cgcggtggaa tttgctgatc
     3901 gagaagatta tcttcgaggt cgataatttt atcgtgcagc tgctcgataa attcgctgga
     3961 atgatcggtc aacgcatcgc acacatcaac cagccacccc ccgcaatcgg tcggaccagt
     4021 gccctcttcc agatcgctca ccacatcgtc cagcgccagc actttgcgtt gtcgggtcga
     4081 aacaattaac cgcccgtcca tatatacacg catggcgacc agttgatcgg ggcgttcatc
     4141 ggtgctgccg tttatacagc gcaatgtaat cagcgtgcct tcaccgagac gactgactcg
     4201 gggccgtgtg ctctcgcccg ccagcgcatc acgtacgtta ttgggaagca gcggtgttgt
     4261 cgccagccat tgggcgctat catggtgtac ataattaagg tggagccagc agggatgcgc
     4321 ttcatcaatc acatctgtat tttccagcgg tttaacgccg cctctaccat ccagcatcca
     4381 tgcaaatact gcatccggga cattaacgtc cgatccctta atcgcttcca cagtgcctcc
     4441 atcatcaacg cattattttg tagtctagcc ttctggccct gttacgcaac atctcatcac
     4501 cccattaccc tgaaatgatt aataaaattc tgtctaaatt gaatacaaaa agcaaaatgc
     4561 ttttccgtat acaaaccgtg tgaagtgtta aatagcgtct atcattatca gaattatctg
     4621 atcatatgac gtggcttttt tgcgatcgga tagcaacaaa aattgataaa aataatggga
     4681 tctcaatgat tacacacaac ttcaataccc tggacttact caccagtcct gtctggatcg
     4741 tttcgccctt tgaggaacag ttaatttatg ccaacagcgc ggcgcgactg ttgatgcaag
     4801 acctcacgtt tagtcagcta cgaaccggtc cctattccgt ctcctcacaa aaagaactgc
     4861 cgaaatacct ctccgatctg caaaatcaac acgatattat cgaaatcctc accgttcagc
     4921 gtaaagaaga ggaaacagca ttaagctgtc ggcttgtttt gcgagagctg acagaaacag
     4981 aaccggtgat tattttcgaa ggtatcgaag caccggcaac gctgggttta aaagccagtc
     5041 gctcggcaaa ttatcagcgc aaaaaacaag gtttttatgc gcgctttttt ctgactaact
     5101 ctgcaccaat gttgttgatt gacccctcac gagatggcca aatcgtcgat gctaacctcg
     5161 ccgcgctcaa tttctatggt tataaccatg aaacgatgtg ccagaaacat acctgggaaa
     5221 ttaatatgct cgggcgtcgc gtcatgccta tcatgcatga aatctcgcat ttacccggtg
     5281 gtcataaacc tttgaatttt gttcataaac tggcggatgg ttcgactcgt catgtgcaga
     5341 cctatgccgg gccgattgaa atttatggcg acaagctcat gttatgtatt gtgcatgata
     5401 ttactgagca aaaacggctg gaggagcagc tggaacatgc tgcccaccat gatgcgatga
     5461 ccggattact gaatcggcga cagttttatc acattaccga gccaggccaa atgcagcacc
     5521 tcgccatcgc tcaggattac agcttgttgc tcatcgacac cgatcgtttt aaacacatta
     5581 acgatctcta tgggcattct aaaggtgatg aggtgttatg cgcccttgcc cgcactctcg
     5641 aaagttgcgc tcgcaaaggc gatttggtgt ttcgttgggg aggcgaagag tttgtcttat
     5701 tgctaccaag aaccccactg gataccgcgc tttcgctggc tgaaactatc cgcgtaagcg
     5761 tggcaaaagt gagtatttcg ggcttaccac gctttactgt cagcattggt gtggcgcatc
     5821 acgaaggaaa tgaaagcatc gatgaactgt ttaaacgcgt tgatgatgct ttgtatcgag
     5881 cgaaaaatga tggacgcaac cgcgtgctgg cggcataagc cgcggatgcg tctcgtgatc
     5941 aacgactgcg cttagcgtgg cgctcccagt tttcttgctt cgcctgcgct gttttacgta
     6001 gtgcgacgta acacgccccg ctgccgccat gatgcggtag cgcggtgcaa tatgcctgaa
     6061 catcatcaaa ttcggtcagc cagcgcgcca catagctgcg gacaatattg gcatgcgatt
     6121 tatcatcccg tcctttgcca tgaataatca gcacgttacg caaaccatcc gccagggctt
     6181 gttgaatgaa actgaacatc attctgcggc actcttccac cggctggcgc aaaagattca
     6241 ggctcgcctg ttgcggatat ttaccactgc gcagcttatc cagcacccca tgttgcaacc
     6301 cttcccgccg aaactccagc ggctgactta gcgggatgat gtcgagaaat ccagtggtga
     6361 ggaaattatc aagttgcagc gtgtcgatac gctgcggcgc acgttggtta cgcgttggat
     6421 gccagtggac atctgtagca cgtttcagcg gctggacatc ttccatggcg tcaagaaaca
     6481 gcgatttgtc gtcaaggttc atgtaacctc ctatgcacct tcacacgtcg tgattcagac
     6541 gggttaattg ttcatactta ctgcatcgtt cccttcacag attgaaacaa tactctactt
     6601 catacctgtg agtatctgcg caggcggggc attttatact agcctgttag cagtatcgaa
     6661 cagtttagcc cgagtttaat tcagcacagg ctccgatcag gatgatttat tcatagcagg
     6721 aaccaacaaa aatgcgataa ataacagacc actgattagc agaaaggtat ccgaaaatgc
     6781 catagtttgc gcctccctca gcatcaattg actcaataat ttggttgaag ccagtatttc
     6841 cgatgtctgg tcacttccac tgcgattaaa aaacaaagca cttctggaaa tgaagtcatt
     6901 caccgtatgg ggaacagaaa ccattttttc acccattctc gaaaaatgaa aattagttcg
     6961 attattgaga atagagccac acagggcaat accaattgcg cccccaagat tacgtgttaa
     7021 attaaataca ccagaggcta acttcagccg ttcttttgga attcccccca gcgttaatgt
     7081 tacgatgggg gccatcgcaa actgctgcga aatccctcta attgcctgtg gaaacagtaa
     7141 ctcctgccat ccccattcgt gggttatagg ggtaaaaaga tacattgaaa acacaaatcc
     7201 gcccagcccc gccatcaaca gccattgcag atttattttt ttacttaacc agaagtaaaa
     7261 gggaacagaa aataactgga aaatacctgt tgtgcaaacg gcaaaaccta tctcttcggc
     7321 atttaatccg cgaacctgcc ccaaaaacac aggtattaaa tatacagtag agaatattcc
     7381 aacccctcca gaaaaagaaa agaaacatcc cagtgtgaaa tttttatcct taaaagcatg
     7441 aagatccatt atcggattgg atattttaag tgtacgcgca gcaaataaaa taaaactcac
     7501 caatgccaga actgatgtca gcaaaatagt attatcatcc aaccatcccc agcgcgctcc
     7561 ttcttcaagg gtatattcca gacatcccag tgtcatggcc aacaggataa tactcggata
     7621 atcagcaact ttcagtaaag agagatcagg tttatcaaaa ttaaccagaa aaggaatact
     7681 caaaactaaa tatatgcccg gtaatatatt gatataaaat agccatcgcc agtcgaggtt
     7741 atcagtgatc cagcccccca gagttggccc tagcgtcggt gacagtgagg ccagcgcact
     7801 gactaccgca gcagccaacc ccaactcttt accctggtaa taaataaacg ccgtcgtgaa
     7861 aactaaaggg atcatggatg cgccagcaac tccctgtaaa gcacgaaaaa aaatcatgat
     7921 ctgaatattc catgcaagtc cacaagcgat actcattaaa gtaaatattc ccgcggatag
     7981 agtaaaaagc caacgagtgg aaaatacccg tgacagccac ccagagagcg gaataacaat
     8041 aatctcggca attaaatagc tggtctgaag ccaggccatt tcgtctttac ctgcggacag
     8101 accaccacct atttcattta gggatgacga tacaatttga atatcaagct gagcaataaa
     8161 taatccaata cacatactgg cgaaagcaat atgtttacgc atagacatca tctctcatca
     8221 acactgacgg tgacggacag tcccgggcga agaagttgct ttaattcctt tgcgtcgtca
     8281 aatgcaatcc gtaccgggac acgctgaaca attttggtga aattgccagt ggcattttct
     8341 atcggaatca agctgaaact ggctcctgtc gctggggaaa ggctttcaat gtgaccgtga
     8401 aacacttccc ctttgagaat atcagcacgg atctcagcct tcattcctgg cttcattccc
     8461 gctatttggt tttctttata atttgcatca acccacaact cactgactgg tactagtgaa
     8521 actaacgatg tgccgccttc cacccaactg cctgtatggg cgctgcggtt tgcgacaatg
     8581 ccatcaatcg gcgcacgaac aagggtatat tccagatcca gtagtgcctg agtcaaagcg
     8641 gcctgaactt cttcgatact tgcacgtacg ttttcttcct gagcagataa aactgccagc
     8701 cgttgcttct ccaccaggaa atcgttcgcg gctttgcgct cctttgctgc gacctgctgg
     8761 tagtcaaacc tggcgttatc aataatttgt tgcgatattg ccgctgattg tgcgagtttt
     8821 tcataacgtt cagtatctcg caggcgcttt tgctcctcat gttttactgc ctgccatgtt
     8881 tcactggcac tttgaattat cgactgctgc atcgcaatag tggcttgaat gctttcgaga
     8941 ttggctttgc tactttttat ttttgcctca agcctggcga catttgcacg ataatcccga
     9001 tcatcgagac gcaggataat atcgcctttt ttacactctg attatcgcgc acctctatag
     9061 cagaaatata accagaaact ttacttgcca ccgtggtgat attaccacca atgtaagcat
     9121 catccgtcgt ctgtataaat cttcctttgc ttacccacca gataaaaaag acaacgccag
     9181 ccaaaagaat accgatcgct accaccccaa taagctgttt tttagaaatt atcatcactc
     9241 tttacaccgt atatatccgg aaacttgcat acgtcatatt taaaatttaa catggcttat
     9301 ttgatgattt ctgacaaaat acctattgta aatgacaatc acactgcaac ccaaagcatc
     9361 accagggcat cacattatcg gactggattc ggaacatctg aatggaggca cagttccctg
     9421 gcataaacat ctctatgccc agttgcttta cccggctgaa ggagtcgttc gtatatgggc
     9481 tggagaaagc gtctggctag tgcatgccag cagcgcgctt tggctgcccc cacaaatgcc
     9541 gcataagttt gttgcgacag gcaacgtact tttaaaaacc gtactggttt cagaagcgga
     9601 aagtgaaacg ctgggaaaag tttgcttcat gacgggtatt tcacccctgt taagggaatt
     9661 actaattgca attaaccaac ttccaccgtc gcaaagtacc acagataaac aacagttacg
     9721 tttttcagcc ctcgagacac tgatacttca ggaaataaaa atgggggtga aaatgtcact
     9781 tgaactccct tggccaaatg atgagcggct acagcagctt tgtgagaatt tgctaaacaa
     9841 ccaagggtat ctgccaacac tggataatct agcggataaa attaacgtca gtagccgtac
     9901 actcatgcgc ttatttgtta aagagacggg attaacattt cgccactggg ttcagcaaat
     9961 gcacgttatt tctgcagtga cgcttcttga tgatggatat tcgttgacca aaattgccca
    10021 tcgtctgggc tatgcctcag ctgaatcgtt cggcaatatg tttaagcgtc gaacgggcta
    10081 ctcacccggt aaatttactc gtcgattaac gatgcatgat tatgcaatca caagacaaat
    10141 gatttaactt atattttcgt gaaatctgtc actgaagaaa attggcaacg gatgggtaaa
    10201 accgttataa cactgtcacc ggtcaccagg accccaggcc ggatttaaga cgaggatgca
    10261 ctgctgtgtg tactgtagag tctggcggat gtcgacaggc tctatttttt tattcatttt
    10321 ttagctctgt aaataaccac attctcgccg ggattgggga atgagtgacg agatcagtgg
    10381 tgtctggcgg ctatcacgcc acagatcaaa taacagatag cctacggtaa aatttcgctg
    10441 actggaattt gatggcttgc cctcatttct tcaggcagaa aaatcaaaaa atcgctttat
    10501 gcttaaatac tgcgacaaac cagacgctaa acctggtact tactttttgt tagtattaat
    10561 ctttttggtg gtatggctgc tgatgttgct ggcgtggtat cttgttggct tgccgatagg
    10621 tccgggtatt tacccacgtt tgtcttaaga gagaacggat gctgagatta cttgaagaaa
    10681 aaattgccac gccactgggt ccactgtggg tgatttgcga tgagcaattt cgcctgcggg
    10741 cggttgaatg ggaagagtac agcgaacgca tggtgcaact gctggacatc cattatcgca
    10801 aagaaggcta tgagcgcatt tctgccacca acctaggtgg tttaagcgac aagcttcgtg
    10861 aatattttgc cggtaatctt agcattattg atacgcttcc cactgccacg ggggggacgc
    10921 catttcagcg cgaagtctgg aaaacactgc gcactatccc ctgcgggcag gtaatgcatt
    10981 atggccaact ggctgaacaa ttaggccgcc ctggcgcggc gcgtgccgtt ggtgcggcaa
    11041 acggatcgaa tcccatcagc atcgtcgtac cttgtcatcg ggttattggc cgaaacggca
    11101 ccatgaccgg atatgcaggc ggagttcagc gaaaagagtg gttattgcgc cacgaaggtt
    11161 atcttttgct gtaaacatta aacaatttgt gccagcttgt tcacactttt atgtaaagcc
    11221 acccttaaca acttaagggt tttcaaatag atagacatat atttacatct aatatcggaa
    11281 ttctctgctg ttaaggtttg cttagactta cttgctccct aaaaagatgt taaaattgac
    11341 aaatatcaat tacggcttga gcagacctat gatcccggaa aagcgaatta tacggcgcat
    11401 tcagtctggc ggttgtgcta tccattgcca ggattgcagc atcagccagc tttgcatccc
    11461 gttcacactc aacgaacatg agcttgatca gcttgataat atcattgagc ggaagaagcc
    11521 tattcagaaa ggccagacgc tgtttaaggc tggagatgaa cttaaatcgc tttatgccat
    11581 ccgctccggt acgattaaaa gttataccat cactgagcaa ggcgacgagc aaatcactgg
    11641 tttccattta gcaggcgatc tggtgggatt tgatgccatc ggcagcggtc atcacccgag
    11701 tttcgcgcag gcgctggaaa cctcgatggt atgtgaaatc ccgttcgaaa cgctggacga
    11761 tttgtctggt aaaatgccga atctgcgtca gcagatgatg cgtctgatga gcggtgaaat
    11821 caaaggcgat caggacatga tcctgctgtt gtcgaagaaa aatgccgagg aacgtctggc
    11881 tgcattcatc tacaacctgt cccgtcgttt tgcccaacgc ggcttctccc ctcgtgaatt
    11941 ccgcctgacg atgactcgtg gtgatatcgg taactatctg ggcctgacgg ttgaaaccat
    12001 cagccgtctg ctgggtcgct tccagaaaag cggtatgctg gcagtcaaag gtaaatacat
    12061 cactatcgaa aataacgatg cgctggccca gcttgctggt catacgcgta acgttgcctg
    12121 attcttccgc ataactcact atccttctgt catatcatta aatttttctg atttattgat
    12181 ctgacagaag gttcatcact gtttcattca ccagatatgg gttaatcttt taattacaaa
    12241 ctgcgttgac agttgttgta aggagaccct gtatggctat gtatcagaac atgctcgttg
    12301 ttatcgatcc taaccaggac gaccaaccag cattgcggcg agctgtttat ttacatcaac
    12361 ggattggtgg caaaattaaa gcctttttgc cgatctatga cttctcatac gaaatgacca
    12421 ccctgctctc tccggacgaa cgtaccgcta tgcgtcaggg cgtcatcagc cagcgtacag
    12481 cctggatcca cgagcaggca aaatattatc tcaatgctgg cgttcccatt gaaattaaag
    12541 tggtctggca taaccgtcct ttcgaagcca tcattcagga agtgatcagc ggcgggcacg
    12601 atttggtgct aaaaatggcg caccaacatg accgtctgga agcggtgatt tttacgccaa
    12661 cggactggca tctgttacgc aaatgcccaa gcccggtgtg gatggtgaaa gaccagccgt
    12721 ggccggaagg gggtaaggcg ctggtggcgg tgaatctcgc cagtgaagag ccgtaccata
    12781 atgcgctcaa tgaaaaactg gtcaaagaga cgatcgaact ggcagaacaa gtcaaccata
    12841 ccgaagttca tctggttggc gcttatccgg taacgccaat taatatcgcg attgaactgc
    12901 cggaatttga cccgagcgtt tataacgatg ccattcgtgg gcaacatttg ctggcaatga
    12961 aagctctgcg acagaaattc ggcattaatg aaaacatgac gcacgtagaa aaaggtctgc
    13021 ccgaagaggt gattcctgat ttggcggagc acttacaggc ggggattgtg gttctgggca
    13081 cagtcggacg caccggtatt tcagcagcat tccttggcaa cacggcggaa caggtgattg
    13141 atcatcttcg ctgcgacctg ctggtcatta aacctgacca gtatcagaca cccgttgaac
    13201 tggatgacga agaagacgat taacgactct tcaataactg acaaggcccg agttatcggg
    13261 cctatttttt acttactgct cttcgccgcc aaggaaataa attcccaacg ggattgccag
    13321 caacacagta aagaccaggc tataaacaaa aatcatcgat gactgaatca catacattga
    13381 tgtcgtccac tcagacagcg gaatattgta ctgttctata acgccgccga tggtcgcgcg
    13441 agtcacaacc gacgccgcaa taatgaaaac cgccaacaaa ccaaagagaa atttcttccc
    13501 tttcgctgac ttcagtttcg ccataatcat gatgtaatcc ttgtgcagac aaatttcttg
    13561 ttacctaaac gtaacaatac catgacattt caccgttgtc tgctctgaaa atctcacctc
    13621 ttagctggat ttacaatcaa cgcattgatt agttagctaa tgttgctact tccttccctg
    13681 actattgatg atctgaatga attgtcgcgg taaaatagtc ccgccagggg tttccggcat
    13741 gacgacaatt atctggagga gcctgataaa acaatgatcg ctgaactgtt tacaaataat
    13801 gcgcttaatc tggtcattat tttcggtagc tgcgcagcat tgattctgat gagcttttgg
    13861 tttcgccgtg gaaatcgtaa aagaaaagga tttttattcc atgcggtgca atttttaatc
    13921 tacaccataa ttattagtgc tgttggtagc atcattaact atgttataga aaactataaa
    13981 ctcaaattta tcaccccagg cgttatcgat tttatctgta cgtccctgat tgcggttatt
    14041 ctgacgatta agctatttct gctgattaat cagtttgaaa aacagcagat taaaaaaggt
    14101 cgcgatatca ccagtgcgcg gatcatgtcg cgcattatca aaatcaccat tattgtggtg
    14161 cttgttctgc tttatggcga acatttcggc atgagccttt ctggcttgtt gacctttggt
    14221 ggtattggtg gtctggctgt cggtatggcc ggtaaagata ttctgagtaa cttcttttcc
    14281 gggattatgc tctatttcga ccgtcctttc agtattggcg actggatccg ttcaccggac
    14341 agaaatatcg aaggtacagt aacggaaatt ggctggcgaa ttaccaaaat taagaccttt
    14401 gataatcgtc cattgtacgt accgaactcg ctgttttcgt cgatcagcgt agaaaatcct
    14461 ggacgaatga ccaaccggcg cattaccacg accattggtt tacgttatga ggatgcggca
    14521 aaagtgggcg ttattgtcga agccgtacgt gagatgctga aaaatcaccc ggccatcgac
    14581 cagcgacaaa ccttactggt ttatttcaac cagtttgctg attcgtcatt aaatattatg
    14641 gtttattgct ttaccaaaac cacggtatgg gctgagtggc ttgccgcaca gcaagacgtt
    14701 tatttgaaga ttatcgatat tgtacagtca cacggcgcgg actttgcctt ccccagccag
    14761 acgctgtata tggataacat tacaccgccg gaccagggtc gctaaatcag acatcactgg
    14821 cgttatccac aagggtaacg ccagttccca tcacattaat gcttcacaat atacatagtc
    14881 cgactgtacg ccacatcttc agggttatta atgggataac ctttcagcca cggcttgatt
    14941 aatcgtccat tggtatattg ataaattggt gcaatcggtg cttgctccat gaggattttt
    15001 tctgccgcgt tgtaatcggc attacgcgct ttaacggtat tttccgtcga tgcctgggcc
    15061 agaactttgt catatgccgg attgttaaag cgtgaaatat tcccggaatg cgttgacgtt
    15121 aataatgtca ggaaagtgga gggttcatta taatccccta cccacgaggc gcggataaca
    15181 tcaaaattgc cggtattacg gctatcgata taggttttcc attcctggtt ttgcaattta
    15241 acatctacgc ccaggttctt tttccacatc gatgctacag caatcgcaat tttttgatgg
    15301 ttttctgaag tgttatacaa aagcgtcaac ttcagcggtt tttgcggacc ataaccagct
    15361 gcgctcaaca aagtttttgc ctgcgcattc agttcttcct gactcatttg ttcaaacggc
    15421 gaaggttccg gcgtaaatcc cgcggtaaca tctggtgtaa aatgccacgc tggcttttcg
    15481 cccgtcccca acactttttc ggtcatcagg cggcgatcta tcgtcatact taatgccagg
    15541 cgaacgcgct gatctgccgt cggccctttt tgcgtgttaa acgcataata ataggtcccg
    15601 agctgcggcg gcgtataaac ctgccccgga atatccttca acagcttctg atacatattt
    15661 ttcgggaagg attcggtgat atcaatatcc cccgcgagaa aacgcttagt ggctgcggat
    15721 tcctgattaa ttggcaggaa ggtcactttt tgcagtaccg ttttggcgtt atcccaataa
    15781 tgggtattcg gcaccacgac cagtttttca ttgactacgc gctctttaag aacataagcg
    15841 ccattgccga tcagatttcc gggtttcgtc cactctttac tgctttctac gttggctttt
    15901 tgcaccggga agaaggcaaa gttagcggtt aaattcacaa accacggcaa tggtttatca
    15961 agctggattt tcaaagtatg ggcatcaact gcggtgacgc caagctggtc aggcgcggct
    16021 ttaccatcaa taatcgcctg tgcgttgttg attcccgcca gcgcggcaaa ccatgcaaat
    16081 ggcgatagcg ttttcgggtc caccagacgt tgccagctgt agacaaaatc ttgcgccgtt
    16141 accggtgtgc catccgccca ttttgcgtta tcgcgcaggg taaaagtcca gatacggttg
    16201 tcattacttt tccactgagt cgcaacgccg gggacaatct cccctttctc gttctgattc
    16261 accagtcctt caaacagatc gcgaatgacc tgaatctccg gcaggcccac ggctttagcg
    16321 ggatccagcg acgctggctc atctttaatg tggcgcacca gctcctgctt ctctgccagt
    16381 actgtgccgc tcggaacttc agcagcatac gaaagagaaa tgctgctgac caacagcgca
    16441 caacacgtga ctgaaacaga gtgcttcata agtttccctc taaccgacat taaaattgat
    16501 gcgtaattat ttgtttcgct ttaaagaaat gcaaacaact tccatcaaaa aagtgacaaa
    16561 ggaagcatca aatgttttaa gtatcaatat atcaaatcag atttagtgta aataatagtc
    16621 tccaaaatgc ggtaaagcac atagaactcc gaacactacg ttcgggcttc gcacattcaa
    16681 acccgttttc ggtatcataa tcagcatctg tcgccattgt gcgaagaata aaaaataacc
    16741 aaaacgttga aaggaactga gaatacgtta agagaagaat taaattaatg tatcagcaag
    16801 tagtgctaaa cagcaccttt atacttcagc ctatctataa ataatgagaa tgccgagcca
    16861 gcatgtcgac ggtgtggata gtacaggtga taaccgggta aatctggtgt gaatttatcc
    16921 aaaacacgta tcagtttttt tcctttaata gcccgctcaa cctgatcata aggtagatac
    16981 gccaatccat gcccatcaat tgcagcatca atgatcaggt ctatcgtatt cagtaaaagc
    17041 tgaccttcca tgcgaacacg aacttcacgt ccaccgcgta ttaatctcca gcgatttgct
    17101 gtacccgatg tgggaagata caaattaatt gcctgatgat ctattaattg tgacactgac
    17161 gttgtagcac ttcgacgaga aaaataatcc ggtgagccaa caatagccat tggtatgtct
    17221 ggcccgattc gaatggcgat catatcttta tccatctccc cacccagacg gacgcctgca
    17281 tcaaaacgtt cagaaacgac atcggtcaaa ccataatcaa tggtaagctg aatatcaatt
    17341 tcaggatgcg atttcaggaa tgtgcgcata gctggtaaca atatcgtttt tgctgcatgt
    17401 tctacagtag taatacgtat tgtcccggat gggcggttct gcagatcgct cagggatgtc
    17461 agggcagaat ctatgtcatg caacataggg ccaagaacag acaaaagatg ctcgcccgct
    17521 tcagtgggta caacgctgcg cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct
    17581 atacggcgca ctatctggct taaagctgac tgcgccatgc tcaggcgggc tgctgcgcga
    17641 gtgaagctac gctcctctgc aacgacgaca aacgccatca gatcagcgat ttcttcacgc
    17701 ttcattcagt atctccatac aatctattaa tcatttaaat gataggtcta atcatgattt
    17761 tgggtctaat caacttaatg atcaatatct atagtgatat caccaaccgt taaaacgaac
    17821 cgacaactcc atgaggaacg gaatgatgaa taataaagtc agcttcacta acagcaataa
    17881 tccaaccatc tctttgtctg cagtgatcta tttccctcca aaatttgatg aaccccttca
    17941 atatccggca attgtggtat ctcacccagg tgggggcgtt aaagagcaaa cggccggaac
    18001 ctatgccgaa aaactggcgg aaaagggatt tgtcactatt gcttatgacg catcttatca
    18061 aggtgaaagc ggcggcgagt cgcgtcagtt agaaaaccca tacattcgta ctgaagacgt
    18121 cagtgccgtg attgattacc tgacaacgct ttcttatgtc gacaatacgc gaattggtgc
    18181 gatgggaatt tgtgccggtg caggatatac agccaatgct gcaattcagg atcgccgcat
    18241 caaggccatc ggtaccgtta gtgcggtcaa tattggttcg atgttccgta atggttggga
    18301 aaacaatgtg aaatctattg atgccctacc atacgttgac gcgggttcaa acgcccgaac
    18361 cagcgatatt agcagcggag aatacgccac tatgccactg gcaccaatga aggagtccga
    18421 tgcgccgaac gaagaactgc gtcaggcctg ggagtattac cacacccctc gcgcgcagta
    18481 tccaacagca ccgggttacg ctactttgcg cagccttaac cagattatta cctatgatgc
    18541 ttaccatatg gcggaagtgt acctgactca gccgatacag attgtggcgg gcagccaggc
    18601 tgggagcaaa tggatgagtg acgacctgta cgatcgagca tcaagccagg ataaacgcta
    18661 tcacattgtt gaaggctcaa accatatgga tctttatgat ggcaaagtct atatggctga
    18721 agcaatttca gtattagctc cgttctttga ggaaactctg taatgaaaca tattcaaatc
    18781 cgtaatagtg atatggcctg gcatattgct gccaataatc tgggaaaagt ttcatgaaaa
    18841 atgtactgat tcttggtgct ggcggtcaga tagcccgcca tgtgattaat caactggcag
    18901 ataaacaaac cattaaacaa actttatttg cccgtcagcc agcaaaaatc cacaaaccct
    18961 atcccactaa tagcaagatt attatggggg atgtactgaa tcatgcagca ctgaagcagg
    19021 ccatgcaggg acaggatgtt gtgtatgcca accttacggg ggaagattta gatattcagg
    19081 caaattcagt gatcgctgcg atgaaagcct gtgatgttaa acgtctgatc tttgttcttt
    19141 ccctcggtat ttatgatgaa gttccaggaa aatttggcga atggaataat gcagttattg
    19201 gtgaacccct aaaaccattc cgcagggctg cggatgcaat tgaggcttct ggactggaat
    19261 atacaattct tcgccctgcc tggctgactg atgaagatat tattgattat gagcttacct
    19321 cacgcaatga accattcaaa ggtacgatcg tatcacgtaa gagtgtcgca gcattaatca
    19381 caggtatcat cgataaacct gagaaacaca ttggtgagaa tataggcatc aatcaacccg
    19441 ggactgatgg tgataagccc ttcttcatgt aaatcaatta tatagctacc atcgtactgt
    19501 actatgtgca gtacgattta ttaaacttat taaggtgaac tgatcaatca gatgcgatta
    19561 acatgtgtag aaccagtaag aataaaatac gccatgtata taacattacg acaattctaa
    19621 tatctgcaca tcaatgaaaa tacaaacaac ttccggcaaa aaagtgatac aagatcgatg
    19681 acatagcgtt gaagaaaagg atttgataat ccgcaaaatt tgcacaacac tgcctgtaat
    19741 acttcctgaa ccagagacga tcatgaccgt aactcgccta cgcgccgaac gcggcgcatt
    19801 tccgcccgga acagaacatt acggacgttc attattgggc gcgccgttaa tctggtttcc
    19861 ggcccctgca gccagtcacg aaagtggttt gattctggct ggcactcacg gtgatgaaaa
    19921 ctcttcgatc gtcacgctct cctgtgctct gcggactttg acaccttctt tgcgccgcca
    19981 tcatgtggtg ctgtgtgtga atcctgacgg ctgccagtta gggttacggg ccaatgctaa
    20041 tggtgtggat ttaaaccgaa actttccggc agcaaactgg aaggaaggtg aaacagttta
    20101 tcgctggaac agcgccgctg aagaacgtga tgttgtatta ctgacaggtg acaaacctgg
    20161 ctccgaacct gaaacccagg cgctgtgcca gcttatacac cgcattcaac ctgcctgggt
    20221 ggtctcattc cacgatccgc tggcctgtat tgaagatccc agacacagcg aattaggtga
    20281 atggctggcc caggcgtttg aattgcctct ggtaaccagc gtcggttatg aaacgcctgg
    20341 ctctttcggc agttggtgtg cagatctcaa cttgcattgc atcaccgcag agtttccacc
    20401 catctcctcc gacgaagcca gcgaaaaata tctctttgcg atggcaaact tgctgcgctg
    20461 gcatcctaaa gatgcaattc gcccgtcgtg aactgaagcg ccggttccac atctaccgcc
    20521 agccaggtcg gtccgtcaag atcggcgaaa ctgacctgcg gcaccagcgg taaagcggcg
    20581 ctaatggcac gagaggtaca caacatgcag cccagcatca gacgaaaacc ttgtgcacgc
    20641 gcttcagtcg ccagcgccag cgcttccgtc agaccaccgg ttttatcaag cttaatgtta
    20701 accatctcat agcgcccttt cagcgccttc agattgctac gagtatgaca actttcatca
    20761 gcacaaatcg gtaacggatg aataaagttc tccagcgccg catcgtcctg cgcaggaagc
    20821 ggttgttcaa gcatcgcaac gcctaaatct gccaatagct ggcaacgcgc cgccaaccct
    20881 tctgcacgcc aggattcatt tgcatcaacg atcagcgtcg catcgggcac agctgtgcga
    20941 attgccacca tccgctcact gataagatgg ttatccagct tcactttcag taatttcgcg
    21001 cctgcctgcc agagtgttga tgcactattg gccatctgat caggcgtacc gatgactatc
    21061 gtctgtgccg tggtgactgt ctcgggaagc gttatgccga tcaaatcagc cagcgattgc
    21121 tgctgttttc gcgccgccag atcccacaat gcacaatcca gcgcattacg tgctgcgccg
    21181 gcagggagga ttttttgcaa ctcctcccgt gtcagccctt tctctaattg cggcacgacg
    21241 ctcataattt gcgccattac cgaggcatca ctttctccat aacgcggata cggcgtgcat
    21301 tcaccggtgc ctttaatacc ttcttcttcc agttcaacca ccaccacgcg cgcttcactg
    21361 cgacttcccc gagcaatcac aaacggggta tgtaagggcc aggcctcctc aaatacctta
    21421 acggttctca tctttactcc ttattgtcgc ggtggtctgc ggattaagcg caaatagcgt
    21481 ttgctgtgtt attgacagtt agcataaact aggtgtgacg ttaactatat gtaaacagga
    21541 aagataatca tgtcacaaac cgttcatttc cagggcaacc cggttacagt cgccaattcc
    21601 atcccgcagg caggtagcaa agcgcagact tttactctcg tggcaaaaga tctgtctgac
    21661 gtcaccctcg gtcagtttgc gggtaaacgc aaagtgctga acattttccc gagtattgat
    21721 accggtgttt gcgccgcatc agtacgtaag tttaaccaac tggcaaccga gatcgacaac
    21781 accgttgtgc tgtgtatctc tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca
    21841 gaaggtctga acaacgttat caccctctcc actttccgta acgctgaatt cctgcaagcc
    21901 tacggtgtgg caattgctga tggcccactg aaaggtctgg cagcgcgtgc cgttgtggtt
    21961 attgatgaaa atgacaatgt gattttcagc cagttggtgg atgaaatcac caccgagccg
    22021 gattacgaag cagctctggc tgtactgaaa gcataagtta atatgcctga tggtgttgca
    22081 ccatcaggca tattcacgct tactcttcgt tcttcttctg actcagacca tattcccgca
    22141 acttattggc aatcgcggta tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg
    22201 gataattgcg ataaagctgg gttaataccg agcgttcaaa acggctggtg atttcgtcca
    22261 gcgaaccttc catcgcatct tcgcccacgg ctaccgttgc ggcgtcataa tccggcaaca
    22321 aaatatcctg tggacgcagc tcataaccgt ccagttgtgt cagtgcgcga tagatagcgt
    22381 tctttaactg ccgcacattt cccggccacg cataacgcgt aagtacagta ttcagatcag
    22441 cggccagttt cggacgcggc acgccctgct cgtcggcaaa gcgggcgacg aacagctcgg
    22501 ttaacggcat gatgtcctgc ggacagtcac gtagcggcgg cagattgagc gtcaacacgt
    22561 tcagacgata atagagatct tcacggaaca cgcctttttg caccagttcg accagattct
    22621 tctgcgtagc acaaatcacc cgcacatcga catgcacctc atggtcttcg ccaacccgac
    22681 ggaaggtgcc atcattaagg aaacgcagta atttcgcctg catccgtggt gacatctcac
    22741 ctatttcatc caacagcacc gaaccaccgt tcgcctgctc aaagaaccct ttcttccctt
    22801 ccggagcatg accaaacagt tcactctcga ccgcatcttc cggtatagac gcacagttca
    22861 gtgccaggta aggtttgctc gctctggggc ttgcctgatg acaggcatac gcaaagagat
    22921 cttttccggt gccggtatca cccgtaatca gcagtggcgc gcttagcatc gccagtttct
    22981 gcgcctgttc gacaacatgc ttcattttcg ggctgacagc gacaatttga ctgaaggcgc
    23041 tgacatcctg ggcggcgaca ttttgcaact gacggcccat acgaatcgtt gatcgcagca
    23101 tcaccaccgc accggtcagg acgtgttgat cattttcatc ctgaagataa acaggcgtaa
    23161 tctccatcag aaaattctgc ccattaataa cgacatgctc gttatgcgaa tcttgcggtt
    23221 cgctttccag ccagcgtaaa aaattaaagc cgttaatcaa ttgcgcggcg gtatggttgc
    23281 gcaggcgatc caatttttgc ccaaaaagct gacagctcgc cgggttcgcc atatccactt
    23341 tgcttttcat atcgacagag agcacaggtt caggcaacgc ctccagtaac gcgctcaacg
    23401 ccagatgctc acgttcggaa ggcatccacg ggacagtacg cacatcggta acacctgcaa
    23461 tacggcgtat ttcggccatc agactgctga aactctcaaa ctccagttca gcaaaattga
    23521 ggtagattcg cccaatggga tcaatctcaa taccgcgtaa atcaatgcct cttagcacga
    23581 gtagatcgag taattcgcgg gtcagaccga gtcggtcttc acaaaagact tccagacgca
    23641 tgggaacctt cacctgaaaa aagaacaatt aatatgatga tatgacacta tttgaatagc
    23701 aggaaggttt ctgtcaacaa tcattgacac aaagacggaa aaacgggcac ttcggcgtaa
    23761 agatatccgg tcagcacccg atattgcatt atttttcgct gggcgttttg cctttttgca
    23821 gcgtctcttt cacctgaccg ataagctgac gacggaaatc cccgaggcga ggtttgtcat
    23881 cgtcaatcca cggcagcggg cggcaaagct ccatagcttt aatccccagt cgtgccgtca
    23941 gaagtccagc accaatcccc tgagctgccc gggtagacaa acgcgcagcg agatcttgcg
    24001 acatccagtc catccccact tcgcgcacca gttcgctggc tccggcgaaa gcgatattca
    24061 gcaataccag cttaaacagg cgcaaacggc tgtaataccc cagttcaatg ccatacagcg
    24121 tggcgatgcg attaatcaaa cgcagattgc gccaggcgat aaacgccata tccaccagcg
    24181 ccagtgggct aacggcaatc attaacgttg attccgccgc tgagcggctg atttcgcgcc
    24241 gcgcctgagc atctaaaact ggctggacca ggtgagcata caaactgacc acttcacgat
    24301 cgttctgcgt ttcatggatt gaggcatacc agcgctgcag cgccggatgt gactgatcga
    24361 tgcccgcctg ctgcgccagt ttttcgcaga atgcacggcc tttacccgtg ccgtgactgt
    24421 gcaacaaatc gcgcgcttcg tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc
    24481 gccactctgt taccacagaa cctacgccag cgccgataat caatgcccca gcggcacatc
    24541 cacccagcgc cacccagtcc tgggtttgcc aggcgttcat tgtccactgc acgccctgcc
    24601 cgacaacgct tgcgccaaac agagccagcc cgcccatcac cattttgcgc cacaggctgc
    24661 gtttcggacg caacgctgcg tccatcaccg cttcaacttg cccctcttct tcctgcgctt
    24721 catcgagcgt ggctggggca aaattttgcg cctgattttc gtcaaaggtc tgctgcgccc
    24781 tgaattttgg attctgatcg acctccagtg gaccgtcgaa atcaatacgt ggttttaacg
    24841 gttcggtcat cgcaatttat ctcctattaa aaattccagc gcagcatcaa gacgaatatg
    24901 cggtagcggt ttgtcgacat ccatcacctg cggacgaaac gcctcaaact ggaagccttg
    24961 cttatcccag aatgcctgac ctggcaaacg tgcgggaact tcgccaggat aaacagtgag
    25021 cggtgcgcca tcgctgagtc gattaccgcg cagcgccggg attttctcac cgttaacatc
    25081 aataattccg ctggtggtcg cctgaacaga cgccagcccc agacagtcca tactgatccc
    25141 ttcgaacgcc gcattttgcc aggcatcctg aatcagttgt tgcagcaatg aaaccatatt
    25201 agcgtgctga tcgatggtca catggtccgc tttagtggca gcaaacaata gcttatcgat
    25261 aaccggcgaa aacaaacgcc ggaacagggt acgttgcccg tagtggaaac tttgcatcag
    25321 ctgcgtcagc gccagacgca tatcattaaa tgcctgtggc ccactgttga gaggttgcag
    25381 gcaatccacc agcacaatct ggcggtcaaa gcgcagaaaa tgattcttat agaacccctt
    25441 caccactttc tcgcagtaat aattaaaccg ctcgcgcaac attccggcat tggtgtgctt
    25501 atcggcctgc gccagtttgg actcgcccca ggcatcgaca tctggccatg ggaagaattg
    25561 cagcgcgggc gctcctgcca tatcgccagg caaaacaaag cgtcctggct gaataaagtg
    25621 cagcccctgc tgtttgcagt gatggagata atcggtccac gcggcggcaa tgtctgccag
    25681 ccggttttcg tcggcaggcg ctagcgggtc cagcccttcg cacatcattc gccatttcgc
    25741 cgaccattcg ccgcgctgac cattgagtaa gcccgtcatc tggcgcgacc agctaaaata
    25801 gtcctgtgcc agcatcggca ggtcgagcag ccattcgccg ggataatcca caatctcaag
    25861 atacagcgtg gaggtgtcct taaagtggcg cagcagcgaa tcgttcgatt taaaacgtaa
    25921 cgccaggcga atttcactga cgccgcgcgt aggcgtcggc caggcgggag gatcgccata
    25981 taactgcgcc aacccttcat cataggtgaa gcgcggaatg ccaaagtcac gctggggaat
    26041 gcgttttacg cccagcaggc gctcttcacg caccgcactt aaaagcggca aacgtgctcc
    26101 ggcatgaata ttgagcaact gattaaccat cgcggtgata aacgctgttt tgccgctgcg
    26161 gctaagtccg gttacagcga ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt
    26221 gagttcattt ttaagtcgct tcattgctgt cctgtgctgc tctgaagacc agaaaaaaaa
    26281 atcgttcgta aggatatgaa tgtgggggcc aggccattga ttatcaacta cccgtgttgt
    26341 catcgacggg cgaactccat aagatgtgca gcattcacgt tcggacggag ttcctgacct
    26401 atgtccccta ctatttatga tattgccagg gttgcaggcg tatcaaaatc caccgtatca
    26461 cgcgtgctga ataagcaaac caatatctcc ccggaagcgc gcgaaaaagt gttacgggcc
    26521 attgaagaat tacagtatca accaaacaag ctggcccgcg cgctgacctc ttcgggtttt
    26581 gatgccatta tggtgatttc tacccgttcg accaaaacta cggcgggtaa tccgtttttc
    26641 tctgaagttt tgcatgccat caccgccaaa gcggaagaag aaggtttcga cgtgatatta
    26701 caaacgtcgc acaatccggc agaagatctg caaaaatgcg aaagcaaaat taagcagaaa
    26761 atgattaaag gcattattat gctaagttcg cctgctgatg aatccttctt tgcacaactc
    26821 gacaaatacg agatcccggt ggtggtgatc ggtaaagttg aaggtcaata cagccatgtt
    26881 tattccgtcg ataccgataa ttatggcgac agtattgcgc taaccgatgc gttaattgaa
    26941 agcgggcata agaatattgc ctgcctgcat gcgccgcttg atgttcatgt ttcagtggat
    27001 cgggtaaatg gttataggca aagcctggct acgcataata ttgcagtgcg tgatgaatgg
    27061 attgttgacg gcggttatac ccatgagaca gcattaaaag cagcacggga attattaagc
    27121 cagtcaccgt tgcctgaggc agtgtttgct actgacagcc tgaaattaat gagtatttat
    27181 cgtgcggcgg cagagaaaaa cattgctatt ccgcagcagt tagcggtggt gggttatagt
    27241 aatgaaacgc tgtcgtttat attaacgcct gcaccaggcg gcatcgatgt tccgacgcag
    27301 gagttaggtc aacaaagctg cgagttatta ttccagctaa ttgccggaaa accgtcacca
    27361 caaaatatta ccgttgctac gcatatgtcg ttgaaataaa tatatgccgg atttggcgct
    27421 cgccccgcat ccggcaattt agcacattat cagaacgagt aattcacccc aacacccgca
    27481 taatggaatt tatcgctgtc gccttcgtcg tgatcctgcc actcaaacgc gtattccagc
    27541 gaaacggaaa gaccattctg gaaatcataa ccgtaaaata aacccacacg gttaaaatca
    27601 tggccttcac gttcaatatc atcctgccag tcccagttac tccagcgatc cagcccaatg
    27661 cgcgtatacg gcgtcaccga gtggttgccg agcgtcagcg gcaaataggc gcgaatttct
    27721 tgcgtggaaa actcaccgtt attgcggcta tcatccatat tgaaaccacg ctcgagataa
    27781 tagttcactc gcaaggcaag cgtttcgttg aaggtatatt gcagacctgt ttccgtttcg
    27841 acacgggtat cagcataacc ggtcgtattc agatcgttgg caaatttata catcgacagc
    27901 caaccgttga aacgtaaatc gtcagtcagt ttcacatccc agtctggcgc aattttccag
    27961 cgctgcatat tcgccgtgtc tttacccggt tcatcaacgt agtgataacc ataattacgg
    28021 aaaccaccag tcagaccgaa actgaaatca tcgctttcga ggaactgata atgtacctcc
    28081 agctccgggc gatcaaacca cgttccacgt ttacccgcgc tataatctac cggcccttcc
    28141 tgataatagg ccagagaaat tctccacggc ccgttggcgg cattaaaata gacagaaggc
    28201 tccgccagcc catccatatc ttcgccataa ccctcaacgt tttctatttc gtacatcgcg
    28261 ccgatattaa agtgccagtc gttcttttcc tcggcctgtg cgcaggccat tcccgcacac
    28321 atcaccagtg cggtacaggg taataacttt ttcattattg ttccttgtat tatcctggga
    28381 gaatgccttc cccctgtatt tagcaaattg ctatttccgt ttctgcatca aagaaatgac
    28441 atttagtcat atcaaaatga atagtgatat tttctcctgc atgataatca tttaacgcac
    28501 cagcacggac cactaactcg tgccccccaa ccgtggtgta gagcataaat tccgcaccgg
    28561 ttaattccgc cacgctaatt ttggcggaaa tgttattttc ctcttgtgcg tccggatgaa
    28621 tatcttccgg gcgtattccc attacgatgg gcttatgcaa actttcctgt gttttcagaa
    28681 ccgctaattt ctcttcgggt atggttaatt taagcgtttc cgtaacgaat ttatcgccat
    28741 cgatcgtgcc gcgaataaaa ttcatcgctg gtgatccaat aaatccggca acaaacatat
    28801 tcgcaggttg gttataaacc gttttcggcg caccgacctg ctgaacaatc ccatccttca
    28861 taatgacaat ccgcgtcgcc atggtcatcg cttcggtctg atcgtgggtc acgtagatca
    28921 tggtggtgtt cagtttctga tgcagcttgc tgatctctgc gcgcatttgc acgcgcagct
    28981 tggcatccag gttagagagc ggctcatcca ttaaaaacac gcccgcttcg cgcacaatcg
    29041 cccgcccaag cgccactcgc tgacgttgcc cgccggaaag cgcgcccggc ttgcgtttca
    29101 ggtactcacg caggccgaga atttgcgccg cccagttcac ccgctcatca atcacctctt
    29161 tggcgatttt ttgcatcttc agaccaaacg ccatgttgtc gtagaccgtc atatgcggat
    29221 acagcgcgta gttctggaac accatcgcga tattgcgtgc tttggctgga acgtcattca
    29281 ttcgtttgcc gtcgatcaac agatcgccgc cgctgatctc ctcaagcccg gcaatcatgc
    29341 gcagggtggt cgatttaccg cagcccgacg ggccgacaaa tacgatgaac tctttatcgg
    29401 caatttccag gttgaagtcc ttcaccacat gcacctggtt atcgtagatc ttttgaatat
    29461 gttgtaacga aagctgtgcc atgttgattc ctttgctata cgttttgcca gaaggccgat
    29521 aaccgcggcc aggtcaggga gtccgttgag ggcaacaata attgcgcccc cgttaagcct
    29581 gcgccgatcc ccaccgagcg cataccgctg gcgttaatgg cgtcaatgcc cgcctgcgca
    29641 tcttcaatgc cgatacatgc ctgtggcggc acgcccagtc cggcacaggc ggcgagaaag
    29701 atttccgggt ccggtttcga gtttttaagt tgggaagcgt ccgcgcagaa ggtgaaaaac
    29761 tcacgcagct ccagcgctgc taaaatcgtc ggcgcattca gggagacaga agccagccca
    29821 accggaatct gctgtgctcg gagatctacc agcaaattgt gaatgccagg cagcacagca
    29881 ttaaccgtca gttcgcgcag tgagtggacg taaagcagat ttttgcgata cgccagttgc
    29941 gctcgttcct gtgggttaaa atcgccctct ttacccccgt gctgcagaat tcgccgcagg
    30001 gactcatcgc ggctgatccc ttttaggaat tcgttaaact gcgcatcaat gctgatgcca
    30061 atttcagcgg caatctgctg ccacgcctgg aaatgcagat gcgcggtatc ggtgattact
    30121 ccatccagat cgaaaattac cccttgcagt ttcattcccc ctcctgatgc gtggtagcgg
    30181 tcccattgaa gggcaaaata aaattcccca aacagaaaac agattcttca gcgacgtaaa
    30241 taagctgacc gttcaagcgc agtgaaacag gcgcagaagt tcgaatcgca atacgctgcg
    30301 cgtcgagagt gacctgtaat tcgcagccct gccagaacag agggaaagac aactgttgcc
    30361 actgctcagg taacgccgga ttgagatgta attcaccgtc acgcacgctc accccggcaa
    30421 aaccctgaat cgcccccagc cagatagcgc cagttgcggc agcgtggata ccgtcatcac
    30481 agctatgtgg atcagcacca agatcgattt cagtcccctc gcgccagaac tgataacttt
    30541 gggtcagcag gccacagcgt gcggcaacaa tgccgtggat tgctttactt aatgacgagt
    30601 cgtgaatagt gcgcggttca taaaattgca gattggcaag acacgatgcc gctgagaact
    30661 gctccggcag catgtaattg agcatcacca catcagcttg tttgaggatc tgcatctcgt
    30721 tcacttctgc gcgtgaataa tccagcagaa tggtttgctt ccccgccgcc gctttgtatt
    30781 tcgccagatt aatcaccggc ttagccataa acgaatcatc ctgcggcaaa acgccgtcgg
    30841 gctgaatttc tggcagccgc agttctttga ggtacatttc tgcacgatga ataaacgcgt
    30901 cgtcgctaca gccaaactga cgagcgatat tcagcgcctg ctggacgttg taatacgcca
    30961 tatagctggt gaaggcgtta ttattgacat gttcggtata ttcgtctggc ccaataacat
    31021 catgaatttc cagacggtcg ttaaccctca ccgcgcggct aatccagaac tttgctgtct
    31081 ccagaagtag cgccatgcct tcatgagcaa tgaaactttc atcccccgtg gtccgccagt
    31141 attgaataac cgcccaggcg atatcggcca ccagatgatg ttccgcctgc gcagaggcca
    31201 ctttttgccg caacccggtg cgaatgttaa tggcggcaaa ttccggcgtc tcttcttcgc
    31261 cgctgcgcgc gctttcccac ggaaatagcg cgccctgcca gccgttgcgt cgcgctttct
    31321 cctgcgcgcc tgtcaagttg tgccagcgat aacgcagtaa acttctggca atcgtcggtt
    31381 cgctaaacag atggaacggc agtaaaaata cttccgtatc ccagaaaacg tggcctttgt
    31441 agccttcccc ggtcaaacct tttgccgcaa tactgctgcg ctcgtcgtga gcaggcgtca
    31501 tgatgcgtag gtgataaagc gcataatcta acgcttgctg atcgtgcgca tcgccgccgt
    31561 ttaccgtgat acgacgtttc tgccaccact gacgccagtt ttctgtggat gctgcaagaa
    31621 gttggtcgta actctgctgc acacacattt caagctgacg aagcgatgca ctaccccact
    31681 cgtctaaagc gacgtgtctg tcgtcccgcc agtcgatcca cacccgtttt tgcagcgaca
    31741 gcgttttgcc cgcaggtagt tgtgcactgg tatgttgcag caagcggcgc tctttggcgg
    31801 tatagcattg ctgcacgtca ccgctcacct ggcagcaaca gctgatggcc acatcactgc
    31861 gtccatcctg agtggtatag atcccctgca tcaaatgctg accaaacacc cgcacctggg
    31921 tttcgtcgag atgttgtcgt ccgtggttgg tttgcgtggc gtcaatgcct gttgaaatca
    31981 gcactggcgc gtcagcgtcc agcggcgtaa tgctgatttc cagcgccatc agcggcagtt
    32041 gttctgccga aacaaagcga cggctggcaa tggcataacg tgcgccgctg gctgaagacc
    32101 agacgacatt gcgacgtaat tctccactgg cgaagtcaag ctcgcgctgc cacgtttcgc
    32161 gggatagcgc aaaaagttca ccattcaagg taatctccat ccctatgacg tcaggcaggt
    32221 tcaccagttc gttgatgtca tttttccccg cgcgatgata aagccccgcc agatacatac
    32281 cgcgtgtctg gcgcgtataa tcttcttcat ggctggcgcg aagcccaaga tagccgttcc
    32341 cctgcgccat cagcgaagca tacttgttca gggtatgctg gctgaaatgg ggttccgtta
    32401 acgttactgg cctggtcata attccacacg cgtccctgtt tctgcggatt gatacaacgc
    32461 cgccaccagt tgctggatga tgtacccctg ctcggcgtcg gctatcatca cgggcttacc
    32521 ttgtacgtgg ttgataaagg cttccatact gcgcagatga cggttgtcgt ctgccatttc
    32581 ccgttgcatc agcgtcatta actcaccgtt gttatcggtg tagatatgtg ctggaaacag
    32641 cgtcgccccg gctttatcac cacaaaagct gacgttcata atcgactgtt cgcggatgtt
    32701 gagtgcaaat gaggtctcaa gccacagaat gccgccgtta tgaaattcaa tggtgccaaa
    32761 cagcgaatct tcgacgctgt aggttgccgg gtcccactcg ccaaattgac cgcagctctt
    32821 ttgcgtgccg atcttttgaa agctatgcgc agtcacgctt ttcaccgctg gaaaacccag
    32881 cacatacatc gccgcatcca gcatatgaat gccgatgtcg atcagcgggc ctccgccctg
    32941 caattcttta ttagtaaaga caccccagcc gggaacgccg caacgacgca gggcgcgggc
    33001 ggtggtaacg taaatctctc ccaaaacgcc gttggtcacc tgttcacgca gctgttgcgt
    33061 atcgagtgca aaacgatggt gaaagtcgta ggccagcacc ttgccctgtt tgcgcgcggt
    33121 atcgcacatt tcccgcgcct gttctaccgt catggcgggc ggtttttcgc acatcacatg
    33181 gcagcccgct tccagcgcca tcagggtatg ttcgtaatga aaacggttag gtgagcagac
    33241 gctaaccaca tcaggtttca ccgccagcag catggcctgc ggatcgtccc acacggatgc
    33301 attcccgtat ttttctgcca gcgcctgcgc ctgggaaagg cggctgtcac agacagccac
    33361 cagttccaga tcattgcggg tgcagtagta cgaagcatga accttatccg ccacctggcc
    33421 tgcgccaatt atcgcgaccc gcagtggaga gcttgtcatt gcacttttca caaagacctc
    33481 ttagcaggta cgcaaccagg ccaaagaatc acggtacgcc tgggcaggat tttctgcccg
    33541 gatacgccct tcatacacca catagccctg atagttatcg gcgcgcagct gttcaaacag
    33601 cgcgtggaaa tcgagggtgc cgctgcccgg ctggtagcga tggttatcgg caatatgcac
    33661 atgaccgagc aagtcgcggt tgtcgtgcaa cgcctgcgcc aggttatctt cttcgatatt
    33721 catgtgatag aaatcgccga taatctgtac atgtttaaga tcgttttcga cgatgtaacg
    33781 acgagcatcg gcgagggtgt tgatcatgtg atcctgatag cggtttaacg gttcgagata
    33841 caccacagtt ccagtccgcg cggcgacctg ttccagtacg cgcagggaat cactcaccat
    33901 tttgcggtcg ccgtccaggc tacgcggcga ggtcatcggc ggtaagcgga aggtaaacat
    33961 gccccatgca gccggaacga cgataccttt accgcccact tctgccagcg cttcaagaat
    34021 gcgttcgatc tgctttaagc cattaagtcg acgctcttcg ataaagtcgc caatccaacc
    34081 gtcatagcca ccgcaggcgg tggtcaccgg taaaccggtt tctttgattg ctgctttgac
    34141 ttcttcgaga ttgttgacca gcagtttgcc gtcaatctca aaaccatcga aacccatctc
    34201 tttgatataa cgaaatttct ccagaatgtt ttccggaaag aacgcctgat tctgtgtgcc
    34261 gattttcatt cttttcattc ctttgaatta aaacgtgacg cccattttga tgctctgttc
    34321 cggatgctgg tcgacatact gcatgtagct ttccgggctg ttggcaaagg tcaccaccgg
    34381 atcgattaaa tcttcgcaat tgagataacc gttcatcagc agttcccagc aggtttcttc
    34441 aatacgctta cggctccagc gcggataatc cgggttcggc tcgctgcacg cacgggagaa
    34501 aacaattttg gcgttattga aatgggcttc gcgtccgagg ttaaaacctt cggcaaacgg
    34561 tttggcaaac gcgacatagg agatggtgcc gccataggcc agaccgcgga gtgccgattg
    34621 cagcgcgtcg gcatagccac tggtttcgat aatcacatcg gcaccctgct tgccggtcag
    34681 cgttttgatc tctttaccta cgtcagcgcc aatggggtta agacagaaat ccgcaccgtg
    34741 gcgacgggca atatcacaac gacgggtaat aggatcgacg ccaattacca ccgacgctcc
    34801 agcgcgtttt gccagttgga tggcaatttg accgatcgcg ccaagcccga ccaccactac
    34861 aaaatcccct acgcgtacat tggcatcgcg cacgccgctc atggcaaact gcgccgggtc
    34921 gtagcagatg gcgtttttcc aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt
    34981 caccgcgtta atgatgaccg tttcggaaag cgggccgtag ccgcatacgc tgtcgcccac
    35041 cgcgtagtcg gtaacctcgc tgccgcactc gataatgtcg ccaaccacca tgttgccaag
    35101 ctggaacttg ccaaactcaa tgccgcgcgg cgcatttgcc ggacgcgggg tgaacatctg
    35161 ccattcgcca ttaaagtcgt cattaataaa cgggctggcg gcgcggaaat caaccacttc
    35221 cgtcccgtgt ttcggcgcgc cgaatcgcac gcggatcttc acttcattgg ctgaaatcgc
    35281 ccgatcttca tactcaacca gcgcagcaac gcgcggtgct gtggctacta actttttcat
    35341 gtaaaactcc ttgtaaagaa actggccgct ttagcccttc acgccaccgg cggtcaaacc
    35401 actcttaata aaacgttcag aaagcgcgta cataatcacc accggcaatg cggtcaccag
    35461 cgaggccgcc atcatccgtc cccagatgta atctggcgta ctgaacagcg cgttcaggcc
    35521 caccggtaag gtgaaattgc tggcgctgga aaggaaaatc gacgcaaaca gatagtcgtt
    35581 ccacgccacc ataaagcagt agacgaatac ggatatcagc ccggacattg ccaggggcac
    35641 ggtgatacgg aagataattt gcagacgatt gaggccatcc atcatcgccg cttcttcgat
    35701 ctcatcaggg atggtgtcga agtagctttt cagcataaac acggcggtgg gcagggtttg
    35761 cgtcaccatg gtaatgatca gcgccatctc ggtgtcgtaa atgccaagtg cggtgatgat
    35821 tttgaacagc ggcaccacca gcaaaatgcc ggagaacatg tacaccgtgt aaaagctggc
    35881 gttgatggtc atccgccctt taaagcgcag gcgggaaagc gcgtaagccc cgagaatgcc
    35941 gagaaatacc gccaccacgg atgaaaccac cgacaccacc atgctgttac ggaagtagtc
    36001 gataaacggg aaaatcaccg gattaaaaat gtcgacgtaa tgctccagcg tccactgctg
    36061 tggcagcagc gtaggatgca gcgagatcgc ctcttttgcg ctcttgaacg aggtcatcag
    36121 catcacaaaa aatggaaaca gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc
    36181 gattcgactg agtgtgcatt tatttgttgc cattgaggtt cacccgtttc ctggtcagca
    36241 gaatgacagc gaaaataatg atgaaaagca ccaccgagat cgccgccgct ttccctaagt
    36301 cattaaaggc aaaggcggtt ttgtagagat agacaccgag aatatcgacc ttggtcgtca
    36361 gcagataaac atccgcgaac atgtagaaca tccagatagt gcgcagggtc accaccgtcg
    36421 ccaggaccgg cataatcgcg ggcagcgtga cgatgcgaaa ccgctgccag gcattcgcgc
    36481 catccatctc tgccgcttcg tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg
    36541 agataaaggc atacgggaag tagcgccaga tagcgaacaa caccaccagc acaaagctgc
    36601 tgcccgggtt atcgaaccac agcggcgcct gctcatagag atgcagaaga tcgacgccga
    36661 ggtagttcac aatgccgtag ccgttgttga acatgtattt ccaggcgaac accagcgaaa
    36721 tagacggcgt tacgtaggaa aggatcacca gcgaacgcgc ggttttgcgc aggcggaact
    36781 cgcggttgaa aaacatcgcc acggccagcc cgagcacggt gctgcccgcc accacaagcg
    36841 cggtatacca gaccgtcatc cacagcgagt gccagaagcc cggatcggag agaatacgca
    36901 catagttgct caccccaaca aatgttgcct cgatattggg attgagcagc agacgtaaaa
    36961 aactgatttc gatattcgac accatcggcc acgccaccag accgcccagc agtaataagc
    37021 tgggcgcgag aagcagcagt gcaaaaggca tatcggaacg acctgaaaaa agcctgttca
    37081 ttgcggattc ctgtcctggg tttagtgctg ttcgatcagt tcatccagct ttttctggct
    37141 cgcttgcagc gtgccaggga gatcggcttt acccacggtg acgttatgca ccattgaact
    37201 caccacgcca gaccccgtca catcgcccat gcgggtaaag tttttatccc ctactgcgcc
    37261 aaagacctga atatttggca gttcgctgat tagctgattc ggcaattctc ccagcgcctt
    37321 aataacgtcg ttgtctttcc aggtggcggt agtcaccacc gctttgttca ccggcagcgc
    37381 cgcgcctggt gacatcatca cccagtcggc aatgttgtcc gcctgctcca taaaggtgac
    37441 aaatttttca gccgcttccg tctcttcagt cttttgcccg gcggtaatgg tcagcgaggt
    37501 caacatgccg tagaccgcag aatttttctc ggttggcacc acgaaaccaa cgtttttcgg
    37561 atcgtcttct ttaatcacag ccggaaggat ataggtggag taaatcgcca tcggcgcggt
    37621 gccgttcata aaagcgtctt tcacttccat gatgtcgtta gaacctggca tggtgttggc
    37681 agcaaggttg cggtaatagg tcagcgcctg catcatctct ggtgtatcaa gggtgatttt
    37741 gccttcggcg ttaaagacgt tagcctgatt ggataacgca aactgggaga aagactgttc
    37801 cgtcaacacg ctttctgctg taggcagcgc aataccgtat tttttattcg ccgggtcatt
    37861 cagtttctgt gcaacgtcca gcaattgctg ccagtttttt ggctcctcca gcccagcttt
    37921 tgccagcaca tctttgcgat accagatccc gccaatccat gcgctgacag gaacaccggt
    37981 ccaggcgctg ccatcttcgg tgcgcacaat ccgcagtacg ccatcgtaga acgcgccttc
    38041 accgacgttg ctgatgaccg tggcaaccgc ctggcgatcg ataagctgct ctttgtccat
    38101 cactttggcg tagtcatggc tggtttcgat cacttccggc agcgaaccgc tacgtgaaag
    38161 ggtaatgact ttggtgttat aggcgtcttc ttccacgggc acttgtttta cggtgatacc
    38221 tgggttttct ttttcaaaac gggcaatcaa tttgctgatg acggcctggc gctcctgctc
    38281 caccgaagaa tgcataaatt cgatggatac attattttta ttttcttctt tacagcctga
    38341 aatcagggcg caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgaaatg
    38401 ttccttttta tttaatccac ataacctgcc agggatgcag tgttatttca ttacccaata
    38461 tatcaacttc actaatcaga tcccgaccgt gcaggtcagt aatatttata tgctgaatat
    38521 tttcactgac attaaacaat cccgtcaggc aatcgccatc cgcattgctt ctttgaattc
    38581 gcattacaaa tgaattaatc gcatcgatgg tgaaattatt atccggatga aactctttat
    38641 gactgcgacg aagtgtaatt aaatgcgaca acttataata taccgaatac cttaatgtcg
    38701 ctttattgtt cagttctgtc gttatctctt tactagagta ttttttacgg ttaatcgcac
    38761 gattatatcc aagtttttcg acacctgcgt aatcattacg cgaccccaga atgctttgaa
    38821 tatatatcgc tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg
    38881 cacaccgttc ttcgtcgcta cactcacggc ggctcaacgc atccatatag gtgacattca
    38941 tttcataggg gctacgcgta ccgtcgggat tatttttcca gtttactaat gccccttcct
    39001 gctgtaacac ctcgaccagc gctaatattt cgttttcggg caacaacccc cgcagtgggt
    39061 taagcccgat gccatcgtga gaggcaagga agttaaacca ggtggtgttg ctggaaggta
    39121 gtgtcaggtt ttgcgcccac gcacaaagtg cctcaacgtt ctgtttttgc accgcatgca
    39181 gcaccagcgg cggcatcgag aactggtaca ccatatgcgc ttcgtcatcg ccttcgccaa
    39241 agtaagcaat gttgtcttta tgcggaacat tggtctcggt aatgatcact gtgcctggcg
    39301 cgacgttatc aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga
    39361 tgcagcttgt tcccggctct ttccacataa agccaacggc atccagacgg acatactctg
    39421 cgcccttttc caggtagcac agcaatacat ccaccatcgc cagcaacact tcagggctac
    39481 ggtaattgag gtcaatttga tcgtcgctaa aggtggtcca taaatggcgc attgaatgat
    39541 cgcgcatctg gaatggcgtt aataacggta acgcacgcgg gcgagtgacg gcgctgagat
    39601 cggttttcgg atcaacggca ataaaaaaat cttcaaaacc tggctgctgt tgtaaatagt
    39661 ttttaaacca ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatggc
    39721 tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat
    39781 aatcaattac cgaaaatcca tcatcagatg accatggata aaaaggtaat aaatgaacat
    39841 gtgaaaaaat tctttgcagc cattgagggt aaaactgatt aaatgtgggt agtggcttta
    39901 aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt tcatcccagt
    39961 gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct
    40021 gtaaatgagt cgcggtaaat gttccaccgt agatttcgtc caggtaatcc gtaattttct
    40081 gtttcatgtt gcctttccgt ggtagcggtc ccatgaaacg agagactact ccgcataaaa
    40141 taaactgtca attgagctca aggggtttag tcacgcagga aatctttttt tacctgggaa
    40201 aaagcgtatt tgtgagctga cgcaaataat ctatggggat gtaaataagg tgtgacagaa
    40261 aaagtaaacg gcgcataagc gccgcttagg gtggattctt ttaacctttg accttcggca
    40321 ttgcgatgtc tttcaggccg ccggcgttct caacgtgggt atatcccatc tcgctaagga
    40381 tgtcttttgc ctgccctgac tggcgtccgg cattgcaata cactttcacg gtgtcgtttt
    40441 tatccggaac ggcggtggca atgcgctctt tcacttcctt caggggaata ttgatggctc
    40501 cctgaacgtg ctcttgctga tactgctctg gaacacgaac atcgatccag tgttcagcgg
    40561 cgaaaacggg cagtgaaaac accagtgtca gagcaagtaa gcctttttta aacatgtaag
    40621 cgtccttcta tggatgaaaa ttgccatcca taatacgcct tagctttaac aatttttgcg
    40681 aaattcccct caccttttat aacgccgcgc cagtttattg gcagcccgac ttaacagcgg
    40741 ttccagcgcc acagccagca acattttcag tggtcggcga gcaactgatt ttatcgccca
    40801 gccagctaca cccgccgggc catagcgcag cgcggtaaga agtaccagct tgcctgctaa
    40861 tttgaaacca ggcttcacct tttgcccggc ctgttgccag cgagtattca taactttcct
    40921 cacagttgac ggaaacggct acgtaacgtg aaagtatcgg aagtgacata acgttccatc
    40981 tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga cttcatccag caattcactg
    41041 ctggaaggta gctgctcacc aaaggccatg ttgtccggca ttggatcaag cgcaaatgac
    41101 aaaatgatgt aagcaaccag ggtaaacagc gccagaccga agaaaatcga cagcaccacc
    41161 aggatacgca ccagttttac tggtacatca aaatagttgg caatcccggc gcagacgcca
    41221 cggaccatgc cctgctgtgg aatacgccat aattttttat tgagattaat gcccgccatt
    41281 agcgatccct ccagttcgga tgttctgcat caagaatgga ttccagcgcc tgaatacgtt
    41341 cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg ctgctgctca ctttgcgaca
    41401 attcactgcg accagaacga ttgctgtaat gcagccataa ccagatcggt aaaacaaaca
    41461 gcacaaaaat ggttaacgga atagccagaa atagcgcgct cataagtact ccttagacga
    41521 tgagcggcgg cgcgttcgga cgccgcctgg atgttattat tgattgtctt gcttcatttt
    41581 ggcttttaat tgtgccagtt gctcgctgat tgcatcatcg gctttcagtt cggcaaactg
    41641 atcgtccagc gatttttgtt taccgaagct gtggctttct gcttccgctt ccatctggtc
    41701 gatacggcgt tcgaaagatt cgaaacgagc cattgcttca tccagtttgc cactgtccag
    41761 ctgacgacgc acatcgcgcg acgagtttgc cgcctgatgg cgtaacatca atgcctgctg
    41821 gcgagcgcgt gtttcgctca atttgttttc cagctcgccg atctctttct tcatacgcac
    41881 tagcgtatcg tccaccagcg tcacttcatg ttccaggctc ttaatcagat cggtcagttt
    41941 ctgtttttca attaacgccg cacgcgccag atcctctcta tctttcagca gtgccagttc
    42001 cgctttttcc tgccattcaa tttcacgcgc cgacgcttgt tcaatacgac gagtcagctg
    42061 tttcttttct gccagcgcac gcgccgaagt agaacgtact tcaaccaatg tatcttccat
    42121 ctcctggatc atcagacgca ccagtttctg tggatcttcc gctttctcta acagagcgtt
    42181 gatgttggca ttcacgatgt cggcaaagcg agaaaaaata cccataatgt tgtcctcttg
    42241 atttctgcgt tcaggattgt cctgctgaac tgttaatgca atttgcgtgc caatttttat
    42301 ctttttgatt tataaagatc tgattgaaga atcaacagca acatgccagg atgaattagc
    42361 gaattacact aacaagtggc gaatttcatc atggcagaat acaaagataa tttactcggt
    42421 gaggcgaaca gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa
    42481 ccggtgctca tcatcggcga gcgcggcacc ggtaaagagc tgatcgccag ccgcctgcat
    42541 tatctctcct cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa
    42601 aatctgttgg attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa
    42661 cgtcatccag ggagatttga acgtgccgac ggcggtacgc tatttcttga tgaactagct
    42721 acggcaccga tgatggtgca ggaaaaatta ttgcgtgtga ttgagtacgg tgaactggag
    42781 cgcgtcggcg gcagccagcc attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc
    42841 gatctcccgg cgatggtcaa tgaaggtact tttcgagctg acctgctcga ccggctggct
    42901 ttcgatgtag tgcaactgcc accactgcgc gagcgtgaaa gcgacattat gttgatggca
    42961 gaacactttg ccatccagat gtgtcgggaa atcaagctgc ctctgttccc gggttttaca
    43021 gagcacgcca gagaaacatt gctgaattat cgctggccgg gaaatatacg tgaattgaaa
    43081 aacgtggtgg aacgttcagt gtatcgtcac ggcaccagcg attatccgct tgatgacatc
    43141 attattgatc cctttaaacg ccgtccatct gaagaagcca tcgccgtttc agaaaacacc
    43201 tcgcttccga cgctgccact ggatttacgc gagtttcaga tgcagcagga aaaagagttg
    43261 ctgcaactca gtttgcaaca ggggaaatat aaccagaagc gcgccgctga attgctgggg
    43321 ttaacctatc atcagttccg cgcgttgttg aaaaagcacc agatttagcg aagttgggca
    43381 atacaacttg ccggatgcag cgtgaacgct ttatccggca tacataatca atacgtcgtt
    43441 tgcaggcctg ataagcgctg cgcatcaggc attattgcgt taaccctgct tctgcgtatt
    43501 aatctctcag cgcatcctgc aaaatttgca ttgccgcatc gaattgcgca accgggatgg
    43561 tatgggcttc gttaggcggc gcatatttgt ggtatttgat ccgccgctat cgcaaagtgc
    43621 cgttgtatga tggcgacaga acgccggtga gcgaaacgta atagtcgaat gcgcggcgca
    43681 ttatcctgcc tgagggtaat gcaccgcgcc attaactata aaaatatgta tgacccggat
    43741 cacgagtgct atcatccgcc tttatgctta gcgcattaac cccaggggca aataatgaac
    43801 cacgatatcc cgctaaaata ttttgatatt gccgatgagt acgcgaccga atgcgcagag
    43861 cctgtcgcag acgctgaacg cacaccactg gcccactact tccagttact gctcacccgt
    43921 ttgatgaaca atgaagagat cagtgaagaa gcccaacatg aaatggctgc tgaagcgggg
    43981 attaatcccg tacgcattga tgagatcgca gagtttttga atcaatgggg taatgagtaa
    44041 ttatttaatt gcagacgatc ggtcagtatc gttgcccgga taaagcgttc gcgccgcatc
    44101 cggctaaaaa tgacacgcac agcagcgtgc tttaatgggt ttctcacgat cgtaccgctc
    44161 tgctcctcgc taagttgttc tgtgagttat agttataaag aaatcacggg tacgaatgtt
    44221 gaactgatgg cgacgccacc tgttcaatga ccaaccacac ggccctcatt acgacctctg
    44281 aacacaaacg gtaatggcaa atgtcattaa atattagtaa gatagcctct ttacat
//
LOCUS       DAZKHK010000025        43955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKHK010000025 DAZKHK010000000
VERSION     DAZKHK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.025"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(95..1507)
                     /gene="pykF"
                     /locus_tag="ACHOS5_001416"
     CDS             complement(95..1507)
                     /gene="pykF"
                     /locus_tag="ACHOS5_001416"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP9776249.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1515..1709)
                     /locus_tag="ACHOS5_001417"
     CDS             complement(1515..1709)
                     /locus_tag="ACHOS5_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776250.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHNHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2064..2273
                     /gene="fumD"
                     /locus_tag="ACHOS5_001418"
     CDS             2064..2273
                     /gene="fumD"
                     /locus_tag="ACHOS5_001418"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP9776251.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            2729..3355
                     /gene="ydhY"
                     /locus_tag="ACHOS5_001419"
     CDS             2729..3355
                     /gene="ydhY"
                     /locus_tag="ACHOS5_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP9776252.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            3376..5478
                     /gene="ydhV"
                     /locus_tag="ACHOS5_001420"
     CDS             3376..5478
                     /gene="ydhV"
                     /locus_tag="ACHOS5_001420"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP9776253.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFLKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            5491..6129
                     /gene="ydhW"
                     /locus_tag="ACHOS5_001421"
     CDS             5491..6129
                     /gene="ydhW"
                     /locus_tag="ACHOS5_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP9776254.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIATMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            6193..6861
                     /gene="ydhX"
                     /locus_tag="ACHOS5_001422"
     CDS             6193..6861
                     /gene="ydhX"
                     /locus_tag="ACHOS5_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9776255.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGRHLIK
                     KENV"
     gene            6858..7643
                     /gene="phsC"
                     /locus_tag="ACHOS5_001423"
     CDS             6858..7643
                     /gene="phsC"
                     /locus_tag="ACHOS5_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP9776256.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            7647..8459
                     /gene="ydhT"
                     /locus_tag="ACHOS5_001424"
     CDS             7647..8459
                     /gene="ydhT"
                     /locus_tag="ACHOS5_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP9776257.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVAGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(8471..10075)
                     /gene="ydhS"
                     /locus_tag="ACHOS5_001425"
     CDS             complement(8471..10075)
                     /gene="ydhS"
                     /locus_tag="ACHOS5_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP9776258.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVLLRMHEAVQEIVPYLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHLLALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRQLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLLFD"
     gene            complement(10201..10506)
                     /gene="ydhR"
                     /locus_tag="ACHOS5_001426"
     CDS             complement(10201..10506)
                     /gene="ydhR"
                     /locus_tag="ACHOS5_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP9776259.1"
                     /translation="MATLLQLHFAFNGPFDDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFSDEKSALTYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            complement(10614..10690)
                     /locus_tag="ACHOS5_001427"
     tRNA            complement(10614..10690)
                     /locus_tag="ACHOS5_001427"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10654..10656),aa:Val,seq:gac)
     gene            complement(10695..10771)
                     /locus_tag="ACHOS5_001428"
     tRNA            complement(10695..10771)
                     /locus_tag="ACHOS5_001428"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10735..10737),aa:Val,seq:gac)
     gene            11079..12335
                     /gene="ydhQ"
                     /locus_tag="ACHOS5_001429"
     CDS             11079..12335
                     /gene="ydhQ"
                     /locus_tag="ACHOS5_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9776260.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCAQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEACNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGIQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITDAATQVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNSLKLQPDQDGIGATLQPA"
     gene            complement(12376..13749)
                     /gene="mdtK"
                     /locus_tag="ACHOS5_001430"
     CDS             complement(12376..13749)
                     /gene="mdtK"
                     /locus_tag="ACHOS5_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP9776261.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            13964..14605
                     /gene="ribE"
                     /locus_tag="ACHOS5_001431"
     CDS             13964..14605
                     /gene="ribE"
                     /locus_tag="ACHOS5_001431"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP9776262.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(14645..15793)
                     /gene="cfa"
                     /locus_tag="ACHOS5_001432"
     CDS             complement(14645..15793)
                     /gene="cfa"
                     /locus_tag="ACHOS5_001432"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP9776263.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGFRVAR"
     gene            complement(16084..17295)
                     /gene="punC"
                     /locus_tag="ACHOS5_001433"
     CDS             complement(16084..17295)
                     /gene="punC"
                     /locus_tag="ACHOS5_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP9776264.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            17408..18340
                     /gene="punR"
                     /locus_tag="ACHOS5_001434"
     CDS             17408..18340
                     /gene="punR"
                     /locus_tag="ACHOS5_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP9776265.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(18337..19362)
                     /gene="purR"
                     /locus_tag="ACHOS5_001435"
     CDS             complement(18337..19362)
                     /gene="purR"
                     /locus_tag="ACHOS5_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP9776266.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            19661..19750
                     /gene="cydH"
                     /locus_tag="ACHOS5_001436"
                     /gene_synonym="ynhF"
     CDS             19661..19750
                     /gene="cydH"
                     /locus_tag="ACHOS5_001436"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP9776267.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            19916..21085
                     /gene="ydhP"
                     /locus_tag="ACHOS5_001437"
     CDS             19916..21085
                     /gene="ydhP"
                     /locus_tag="ACHOS5_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776268.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMSIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLASIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAMSMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(21231..21812)
                     /gene="sodB"
                     /locus_tag="ACHOS5_001438"
     CDS             complement(21231..21812)
                     /gene="sodB"
                     /locus_tag="ACHOS5_001438"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP9776269.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21940..22755)
                     /gene="mepH"
                     /locus_tag="ACHOS5_001439"
     CDS             complement(21940..22755)
                     /gene="mepH"
                     /locus_tag="ACHOS5_001439"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP9776270.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNKTASA
                     NFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            23090..23437
                     /gene="grxD"
                     /locus_tag="ACHOS5_001440"
     CDS             23090..23437
                     /gene="grxD"
                     /locus_tag="ACHOS5_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP9776271.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(23654..23716)
                     /locus_tag="ACHOS5_001441"
                     /pseudo
     CDS             complement(23654..23716)
                     /locus_tag="ACHOS5_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(23809..24456)
                     /gene="rnt"
                     /locus_tag="ACHOS5_001442"
     CDS             complement(23809..24456)
                     /gene="rnt"
                     /locus_tag="ACHOS5_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP9776272.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(24559..24966)
                     /gene="gloA"
                     /locus_tag="ACHOS5_001443"
     CDS             complement(24559..24966)
                     /gene="gloA"
                     /locus_tag="ACHOS5_001443"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9776273.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(25047..26144)
                     /gene="nemA"
                     /locus_tag="ACHOS5_001444"
     CDS             complement(25047..26144)
                     /gene="nemA"
                     /locus_tag="ACHOS5_001444"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP9776274.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSIPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(26181..26780)
                     /gene="nemR"
                     /locus_tag="ACHOS5_001445"
     CDS             complement(26181..26780)
                     /gene="nemR"
                     /locus_tag="ACHOS5_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP9776275.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            26883..27122
                     /gene="ydhL"
                     /locus_tag="ACHOS5_001446"
     CDS             26883..27122
                     /gene="ydhL"
                     /locus_tag="ACHOS5_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP9776276.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKPAPSDEPEQPSLF"
     gene            27171..28067
                     /gene="ydhF"
                     /locus_tag="ACHOS5_001447"
     CDS             27171..28067
                     /gene="ydhF"
                     /locus_tag="ACHOS5_001447"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9776277.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            28148..28669
                     /gene="sodC"
                     /locus_tag="ACHOS5_001448"
     CDS             28148..28669
                     /gene="sodC"
                     /locus_tag="ACHOS5_001448"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9776278.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALIVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(28670..30682)
                     /gene="ydhK"
                     /locus_tag="ACHOS5_001449"
     CDS             complement(28670..30682)
                     /gene="ydhK"
                     /locus_tag="ACHOS5_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9776279.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDTTREIL
                     EQLLTALASSQTDVYTIARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(30682..31539)
                     /gene="ydhJ"
                     /locus_tag="ACHOS5_001450"
     CDS             complement(30682..31539)
                     /gene="ydhJ"
                     /locus_tag="ACHOS5_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9776280.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLANAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQMEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(31542..31778)
                     /gene="ydhI"
                     /locus_tag="ACHOS5_001451"
     CDS             complement(31542..31778)
                     /gene="ydhI"
                     /locus_tag="ACHOS5_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP9776281.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            31979..32413
                     /gene="slyA"
                     /locus_tag="ACHOS5_001452"
     CDS             31979..32413
                     /gene="slyA"
                     /locus_tag="ACHOS5_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP9776282.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(32460..32927)
                     /gene="slyB"
                     /locus_tag="ACHOS5_001453"
     CDS             complement(32460..32927)
                     /gene="slyB"
                     /locus_tag="ACHOS5_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP9776283.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            33201..34310
                     /gene="anmK"
                     /locus_tag="ACHOS5_001454"
     CDS             33201..34310
                     /gene="anmK"
                     /locus_tag="ACHOS5_001454"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP9776284.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVVELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGARQETVLGAIFPANP"
     gene            34408..34737
                     /gene="mliC"
                     /locus_tag="ACHOS5_001455"
     CDS             34408..34737
                     /gene="mliC"
                     /locus_tag="ACHOS5_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP9776285.1"
                     /translation="MSMKKLLLICLPVLLTGCSSFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            34796..35452
                     /gene="pdxH"
                     /locus_tag="ACHOS5_001456"
     CDS             34796..35452
                     /gene="pdxH"
                     /locus_tag="ACHOS5_001456"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP9776286.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            35581..36855
                     /gene="tyrS"
                     /locus_tag="ACHOS5_001457"
     CDS             35581..36855
                     /gene="tyrS"
                     /locus_tag="ACHOS5_001457"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9776287.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEDGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            36917..37777
                     /gene="pdxY"
                     /locus_tag="ACHOS5_001458"
     CDS             36917..37777
                     /gene="pdxY"
                     /locus_tag="ACHOS5_001458"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFP9776288.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVKNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(37821..38426)
                     /gene="gstA"
                     /locus_tag="ACHOS5_001459"
     CDS             complement(37821..38426)
                     /gene="gstA"
                     /locus_tag="ACHOS5_001459"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP9776289.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(38532..40034)
                     /gene="dtpA"
                     /locus_tag="ACHOS5_001460"
     CDS             complement(38532..40034)
                     /gene="dtpA"
                     /locus_tag="ACHOS5_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP9776290.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(40645..41280)
                     /gene="nth"
                     /locus_tag="ACHOS5_001461"
     CDS             complement(40645..41280)
                     /gene="nth"
                     /locus_tag="ACHOS5_001461"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9776291.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(41280..41975)
                     /gene="rsxE"
                     /locus_tag="ACHOS5_001462"
     CDS             complement(41280..41975)
                     /gene="rsxE"
                     /locus_tag="ACHOS5_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP9776292.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(41979..42599)
                     /gene="rsxG"
                     /locus_tag="ACHOS5_001463"
     CDS             complement(41979..42599)
                     /gene="rsxG"
                     /locus_tag="ACHOS5_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP9776293.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVINAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(42603..43661)
                     /gene="rsxD"
                     /locus_tag="ACHOS5_001464"
     CDS             complement(42603..43661)
                     /gene="rsxD"
                     /locus_tag="ACHOS5_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP9776294.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLCFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNISGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(43662..>43955)
                     /locus_tag="ACHOS5_001465"
     CDS             complement(43662..>43955)
                     /locus_tag="ACHOS5_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381167.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9776295.1"
                     /translation="PEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAV
                     EAAIARAKARKLEQQQANAVPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT        11229 a        10566 c        11317 g        10843 t
ORIGIN      
        1 tccgatttga ttttttattg cttctggtta tcgattaaat aaaaaagcgc ccatcagggc
       61 gcttcgatat acaaattaat tcacaaaagc aatattacag gacgtgaaca gatgcggtgt
      121 tagtagtgcc gctcggtacc agtgcaccag aaaccataac tacgacatca cctttgtgtg
      181 ccagaccgct ctgcagagcc agttctttac ccagacggta gaagtcatca gtagaagtga
      241 tctctttaac cagctgcggc acaacacctt tgctcagtac taactggtga gccgtttttt
      301 cgttggtggt cagtgccagg atggtggcat ccgggaagta tttacgtact gcgcgagcag
      361 atttaccgcc ctgggtagca accacgatca gcggggcatc cagtttttca gcagtttcaa
      421 cggcaccacg gcataccgct tcggtaatac gcagtttacg gttgtcattg ttgaactcga
      481 gacggctgtt catcacgcgg tcggtacgtt cgcagatggt cgccatgata gaaaccgctt
      541 ccagcgggta tttacctttt gcggattcac cagacagcat cactgcgtca gtaccgtcga
      601 ggatggcgtt tgcaacgtca ccggcttctg cgcgagtcgg gcgtgggttt ttgatcatgg
      661 aatccagcat ctgggtcgca gtgataacaa ctttacgtgc acggatacat ttttcgatca
      721 tcatcttctg ggcgaagata acttcttcta ccgggatttc tacacccagg tcgccacgcg
      781 caaccatgat gccgtcagag gcttcgagga tttcgtcgaa gttgttgagg ccttcctggt
      841 tttcgatttt ggagatgatg tggatgtttt cgccgccgtg cgctttcagg tgctcacgga
      901 tttcgataac gtcagaacgc ttacgaataa aggaagcagc aacaaagtct acgccttgtt
      961 cgcaaccaaa gatcaggtcc tgtttgtctt tttcagccag tgctggcagg gcaatggaaa
     1021 cgccaggcag gttcacacct ttgttttcgc ccaggtcgcc gttgttcagc actttacaga
     1081 taactttgtt accttcaatg gcggtaactt ccataccgat cagaccgtcg tcaaccagta
     1141 cggtgttgcc aacagacagg tcagtagtga aaccttcata cgttaccgca accatttcgc
     1201 tgttgccgat aacagattta tcagtagtga aagtaaaggt ctgacccgct ttcagagaaa
     1261 cgtcgttacc gccttccagt ttcatggtgc ggatttctgg acctttggta tcaagcagga
     1321 tagcggcggt tttaccagtt ttgctcatca cgttgcgcag attctgaatg cgctgaccgt
     1381 gttctgcata gtcaccatga gagaagttca gacgcataac gttcatgcca gcgtccagca
     1441 ttttagctaa catctcttca gattcggttt tcggtccgat ggtgcaaaca attttggtct
     1501 ttttcatgac agtcttagtc tttaagttga gaaggatggg agaaacttgc tttctgggcg
     1561 cactgccgga aagcgaatcc ggtattacga aagtggttgt gcctcgctct aaggataggt
     1621 gacatcgaaa aagcgtgcag aggaatgtgt gcttgtgtct cagcccaacg tgagtgcctg
     1681 tttttgagtc gttgagttct acagtccaaa gtgctgaaac cattcaagag tcaattggcg
     1741 cgcattatac gctgaaatta atccaaaagg aaaacaaaaa cagcgtttca aaaaatatac
     1801 aacttgttag acaataaatt gttatcaaag attcagcgtt ccagggaaaa ggttacgcaa
     1861 aaacttcgtt gtttgcgcaa cgtctggtgc cggggttgtc agtgtgagga taagtgaaag
     1921 ttcatccggt gtataaatac atcatcgctc aattgctaca gttaaaataa tttatcacgt
     1981 tatttgtctg attttgtttg atttttgttt aattggttag attgatatat gctgatagtt
     2041 ggcatgataa ataggtataa agaatgggaa accggacaaa agaggatgaa ctgtaccgag
     2101 aaatgtgcag agttgtcggt aaagttgtgc tggaaatgcg cgatctgggg caggaaccaa
     2161 agcatattgt tatcgcaggc gtattacgta cagcattagc caataaacgc attcagcgca
     2221 gtgagctgga aaagcaggcg atggaaacag tcattaacgc actggtaaaa tgaccattca
     2281 ggatgaggcg gtaagcttca tcctgatatt gttagttcag ttgcggtcca gttaatcatt
     2341 ctaaataaca actgctgaaa ttatacgaat ttcggtgtat attttaattt atagcgataa
     2401 agttattttt attttctgcg atgaacttca caaataattg tcggttaagt taaatttatt
     2461 tcaaatgata ataaatatca gttcacttat aatttcttgc tttgtgattt taattgataa
     2521 cgatcaatgt taagagccca gaataatcac attatttttt ctcctaacca taaaggatta
     2581 gtttatcagc gttaaatctt ttctcataca ctatcgggtt agggaaaata tctggtgttg
     2641 aggacagagt cagcagcgta ttagttgagt gcctaatatc aggctactga attgtcagca
     2701 ctgtgtttgc gaagaggatg cgaaataaat gaacccggtt gatcgtccac tattaaatat
     2761 tggcttaacg cgattagaat tcttgcgtat atcaggaaaa ggccttgctg gtttaacaat
     2821 tgcccctgct ttactgtcac ttttgggttg caaacaggaa gatattgata gcggcacagt
     2881 agggttgata aatacaccca aaggggtgct ggtgacccaa cgtgcgcgat gcacgggttg
     2941 ccaccgctgt gaaatctctt gtaccaactt caacgatggc tcagtaggga cattcttctc
     3001 ccgtatcaaa atccatcgca attatttctt tggcgacaac ggggttggct ctggcggtgg
     3061 cctgtatggc gatctcaact ataccgcgga tacctgccgt caatgcaaag agccgcaatg
     3121 catgaacgtc tgcccgattg gtgcgattac ctggcagcag aaagaaggct gtattaccgt
     3181 cgatcataaa cgttgtattg gctgtagcgc ctgtactacg gcatgcccgt ggatgatggc
     3241 caccgtaaat accgaaagta aaaaatcctc gaaatgtgtg ttatgcggtg aatgcgcaaa
     3301 cgcctgccca acaggggcgt taaaaattat cgagtggaaa gatattactg tgtgaatctt
     3361 gcaaaggaaa aaatcatggc taacggttgg acaggtaata tattaagagt caatctcacg
     3421 acaggaaata ttaccctcga agattccagt aagtttaaaa gttttgtcgg tggcatgggc
     3481 ttcggctaca aaattatgta tgacgaagta ccgccaggca cgaaaccttt cgatgaagcg
     3541 aataaattag tttttgctac cggcccatta actggatctg gtgccccctg tagttctcgc
     3601 gtaaatatca cttcactttc tacttttacc aaaggaaatt tagtcgtcga tgcccatatg
     3661 gggggctttt ttgcagcgca aatgaaattc gctggatacg acgtcattat tatcgaaggt
     3721 aaagcgaaat caccggtatg gctgaagatt aaagatgaca aagttagcct tgaaaaagcc
     3781 gatttcttat ggggaaaagg gacgcgcgca acgacggaag aaatttgtcg cttgaccagt
     3841 ccggaaacct gtgtggcggc tattggtcag gctggggaaa accttgttcc tctctctggc
     3901 atgttgaata gccgtaacca cagcggcggt gcgggaactg gcgcaataat gggttcgaaa
     3961 aacctgaaag cgattgcggt tgaagggacg aaaggggtca acattgccga tcgtcaggag
     4021 atgaagcgtc tcaacgatta catgatgact gaacttatcg gtgcgaataa caaccatgtc
     4081 gtgccaagta cgccgcaatc gtgggcagag tattcagatc ccaagtcacg ttggacagca
     4141 cgtaaagggc tgttttgggg cgcggctgaa ggtggtccga ttgaaacggg ggaaattcct
     4201 ccaggcaatc agaacacggt cggctttcgt acctataaat ccgtttttga cttaggtccg
     4261 gcggcagaga aatacacagt aaaaatgagt ggctgccact cttgcccgat ccgttgtatg
     4321 acccaaatga atattcctcg ggtgaaagag tttggcgtgc ccagcacagg tggtaacact
     4381 tgtgtagcaa actttgtcca taccaccatc ttcccgaacg ggccgaaaga ttttgaagat
     4441 aaagatgatg gtcgtgtgat tggtaacctg gttggtctga atctgttcga tgactacggc
     4501 ctatggtgta actatggtca gcttcatcgc gactttacct attgttacag caaaggggtg
     4561 ttcaagcgtg ttctgcctgc tgaagagtat gcggaaattc gctgggatca actggaagcg
     4621 ggtgacgtta acttcattaa agatttttac taccgtctgg cgcatcgcgt gggtgagctg
     4681 agtcacctgg ctgatggttc atatgccatc gcagaacgct ggaatttggg tgaagagtac
     4741 tggggctacg cgaaaaataa actctggtcg ccgtttggct atccagttca ccacgccaat
     4801 gaagcgtcag cgcaggtcgg ctccattgtt aactgtatgt tcaaccgtga ctgcatgacg
     4861 catacccata tcaattttat tggttccggc ttgccattga aattgcagcg tgaagtggcg
     4921 aaagagcttt ttggttctga agatgcttac gatgaaacca aaaactacac gccaatcaat
     4981 gacgcaaaaa ttaaatatgc taagtggtcg ctgttgcggg tctgtttgca taacgccgtc
     5041 accctgtgca actgggtctg gccaatgacc gtttcgccgc tgaaaagccg taattatcgg
     5101 ggcgatctgg cgcttgaagc caaattctta aaagcgatca ctggcgaaga tatgactcag
     5161 gaaaaattag atttagctgc agagcgtatc tttacgttgc atcgcgccta cacggtaaaa
     5221 ctgatgcaaa ccaaagatat gcgtaatgaa cacgatctta tctgttcctg ggtattcgac
     5281 aaggatccgc agattccggt ctttactgaa ggtacagaca aaatggatcg tgacgatatg
     5341 catgcttcgc tcacgatgtt ctacaaagaa atgggctggg acccacagct tggttgtccg
     5401 acccgcgaaa cattgcagcg tctggggctg gaagatattg ccgccgatct ggcagcacac
     5461 aatctactgc ctgcgtaagg aatgggagag atgaaccatc aggatgaatt gccgttagcg
     5521 gaagtcagcg aaattgatga ggcgaagcgg caatggttgc aggggatgcg tcaccccgtt
     5581 gatacggtaa ccgaaccaga accggctgaa atcctggcag agttcattcg ccagcattct
     5641 gcggctgggc aactggtggc gcgcgccgtg tttctctcgc caccctattc ggttgcagaa
     5701 gaggaactgt cagttttgct ggaaaacatt aagcagaacg gagactacgc ggatatcgct
     5761 tgtatgacgg gaagccagga tgattactat tactcaacgc aggccatgag tgaaaactac
     5821 gcagcgatgt ctttgcaagt tgtggagcag gacatttgtc gcgccattgc acacgccgtt
     5881 cgttttgagt gccagacgta tcctcgtcct tacaaagtcg ccatgctgat gcaagcgcca
     5941 tattacttcc aggaagcgca gattgaagcc gcgatcgcca cgatggatgt tgcacctgaa
     6001 tatgccgata tacggcaggt ggagtcatcg acggcagtgt tgtacttgtt tagcgagcga
     6061 tttatgacct atggcaaggc gtatggctta tgcgaatggt tcgaggtaga gcagtttcag
     6121 aacccctgaa atttcagggg gatgtcatgg atagggtgga cagttgcagc cactgcatta
     6181 ggggataatc agatgtcctt cactcgacgc aaatttgttc tggggatggg aacagtaatc
     6241 ttttttactg gttctgcgtc ttctctgtta gcgaatacga ggcaagaaaa ggaagttcgg
     6301 tatgccatga ttcatgacga gtcacggtgc aatggctgta atatctgcgc ccgcgcatgc
     6361 cgtaaaacta accacgtccc cgctcaggga agccgtttat cgatagcaca cattccagtc
     6421 atcgataacg acaatggaac gcagtatcac ttttttcgcc agtcatgtca gcactgcgaa
     6481 gatgctcctt gcatcgacgt ttgcccgaca ggggcgtcgt ggcgtgatga gcaggggatc
     6541 gtgcgggtag aaaaatcgca gtgcatcggc tgcagttatt gcattggcgc ttgcccgtac
     6601 caggttcgct accttaaccc tgtcactaaa gtggcagata aatgtgattt ctgcgctgag
     6661 acccgactgg caaaaggctt tccgcccatt tgcgtcagtg cctgcccgga acacgcattg
     6721 atctttggtc acgaagatag cccagaaatt caggcgtggt tgcaggaaaa taaatactat
     6781 caatatcagc tacctggcgc cgggaaaccg catctgtatc gccggttcgg tcgacatttg
     6841 attaaaaagg aaaatgtatg aacccgtcgc aacatgctga acagtttcag agccagttgg
     6901 cgaactatgt gccacagttc accccccaat tttggccggt gtggttgatc attgccggac
     6961 tattgctggt tgggatgtgg ttggtgctgg ggctacatgc cttgcttcgt gctcgtggcg
     7021 tgaagaaatc agccaccgat catggtgaga ggatttatct ttacagcaaa gcggtcagat
     7081 tatggcactg gtcgaatgcg ttactctttg tattgttgct ggcaagtggg ctgataaatc
     7141 actttgcgct ggtgggcgca actgcggtta aaagtctggt tgcggtgcat gaagtttgcg
     7201 gatttttgtt actggcatgc tggcttggct ttgttctgat caatgccgtt gggggtaatg
     7261 gtcaccacta tcgcattcgt cgtcagggtt ggctggatcg agcggcaaaa caaacgcgat
     7321 tttatttgtt tggcattatg cagggggaag aacatccttt cccggcaaca acccagtcta
     7381 aatttaatcc cttacagcag gtcgcctatg ttggtgtcat gtatggattg ctgccattgt
     7441 tactattgac ggggctgctg tgtctctatc cgcaagccgt gggagatatg tttcctggcg
     7501 taagatactg gttattgcag gcgcattttg ctctggcatt tataagcctc ttttttatct
     7561 tcggtcatct ttatctttgc accacggggc gtacgccaca cgaaaccttt aaaagcatgg
     7621 tcgatggcta tcaccggcac taacgaatga tcattacacg agcagacctg cgagaatggc
     7681 gtatcggagc ggtgatgtat cgctggtttc tgcgccattt cccacgtggc ggtagttatg
     7741 ccgatattca tcatgcactg attgaggaag gatatacgga ctgggcggag agtctggttg
     7801 agtatgcgtg gaaaaaatgg ctggcggatg aaaacttcgc gcatcaggaa gtttcctcta
     7861 tgcaaaaact ggccacagat cccggagaaa gactattttg cagtcagttt gcccgcagcg
     7921 acgatcatgc tcgcatcggc tgttgtgagg ataacgccag aatcgcaacc gctgggtatg
     7981 ccgcgcaaat tgccagtatg ggttatagcg tgcggattgg aagtgtcggc tttaacagtc
     8041 acattggcag ctccggtgca cgcgcacggg ttgctgtagc cggtaattct tcgcggataa
     8101 gcagcgctgg agatagcagt cggattgcga ataccggaat gcgagtacgc gtttgtacat
     8161 tgggtgaacg gtgtcatgtc gccagtaatg gtgacctggt gcaaatcgcc agctttggcg
     8221 caaacgccag aattgccaac agcggcgaca atgtacacat cattgccagc ggtgaaaatt
     8281 caacggtagt gagcacgggc gtggtcgact caattattct cgggccaggg ggatgcgccg
     8341 cactggcata tcatgacggc gaaagagttc gcttcgccgt tgccattgaa ggtgaaaaca
     8401 atattcgagc cggagtccgc tatcgtctta atgaacaaca tcagtttgtt gagtgctgat
     8461 agctttacct ttaatcaaac aaaagacgcc gacgcgcacg ggatgcaggt tttacgaccg
     8521 ctcgagccat cgcctctcca atttctgcac atgccgtaag tccctgaacg aaaggctggt
     8581 cgtgcatcaa ccagggcaac gcgccgaacg ctacgcgccc acgaatatct tcgggttgct
     8641 gtaacgtata atcttcgcca acatcaggga tttcatcccc tgttttctgt aattgttcgc
     8701 gtagtccagg gaaaggaata tctttcactt taagctgacg ctgcccgcgg gcatcaataa
     8761 aaacgtcaaa cgagtaactg ttgtcttccg ttttcaggac ggtgcgcgac tcgttaattt
     8821 ccatttcgta gtcttcaccg agagcgagaa gatgaatgat tcccgcttca cgtaaggcca
     8881 gtagtcgacg aatagactct gaagggattg ccgcataatt atcgatgaaa acccgggcaa
     8941 ggcctttact gaaccgttta tggtcgtgtt cattcagata cggaacaatt tcctgtacgg
     9001 cttcatgcat gcgcagaagg acataacgcc agggaacagt atgtttttct cgtttattgc
     9061 gttcgacttc ctggagattt ttttctgccc agtcaaatgg gtcgcgttgt ttgcgctcgg
     9121 caaaccaggc ttgagcaaag gaatcgacat tcaggctctc taaggctatg cgttggctcc
     9181 agtctggatc agcaaacttg atttcctcta ctatcaatct aaatacccga tccaaaaggc
     9241 ccacttcgcc tttttgaatc tcagcattta atgcctgatc ggtgatgatg tgtaagggct
     9301 cgtagggaat agggcaatag aaatcggctt cgggtaaaat acctgtgcgc gacattaacg
     9361 taatatttag cttttcactt gcgttatcgc ggtgaaaagt gacgtgttgt ttatcatctt
     9421 caatgaacga accatgctga atagccactg ccattgccgc atccagtccg ctcaaggatg
     9481 ttcccataat ccccacgtta cacgcatcga cctttgcttc catcaggcct gaccacgggc
     9541 tgggaaaata cgttctggtt gcttcttctt catcaggcca gacgtgcccc gttgcgatca
     9601 ccgctaaatc aaacgtctcg ctgggtaaat ccagatttgt agcgagcatg acgccagcat
     9661 ttgtaatttg cagatcggta acctggcatg attcataaac agccactgca aatttttgct
     9721 gtcgtgcctg gtctactaat cgtaaaaatt gatcgcggaa atattcgccc agcagaattc
     9781 gcggtaaaaa ctgacgatcg tgcaaggttt cttttttaac gccataacgc tggagatggc
     9841 tggcttcttg cttttgtagc cattcgagat acgtgcaata aatcggcggt atttcaatac
     9901 tggcaatgtt tgccagcatc agttttgagt tttcctcatc actgtatggc atcccgacac
     9961 cggcttcgtc agcctgctcg aagatagaaa tagaaagtgg cgtttgttgc tgtagaagcg
    10021 agaataaggt gtagatccct gtaggcccgg cacccacaat agcaattttt ttcattgtta
    10081 cttacctgta cgcattcttt cgaatttagt gttatcaggt aagtttggtt gagattttta
    10141 gaatttgaga aatttcagac agggatgaaa agggcactaa ccgcccttga ttaaaatctg
    10201 tcaggcaagt tttgcctgat tgatttgact cagtggcaca ttgacatcga aaactttggc
    10261 gacaacttcc tcaacgccaa gatttttcag tcgggcagta tgtttttcca gataggtaag
    10321 ggcgcttttt tcatcagaga aaaggtagat cccaccggct tcgtggttct tttcactttc
    10381 tgtccaaacc ttccacagaa aaccaggttc ctgattaatc gactcagcaa gcggtttaag
    10441 ctgctcagcc attgcgtcgt caaatgggcc attaaaagca aaatgaagtt gtaacaaagt
    10501 tgccatagcg attctccttc tgataacgca cacgaagaca gtgtagatct tttaacgtat
    10561 tggatacaga gaagaaacag agggacaaag aagaagacca gattaagaaa atctggtgcg
    10621 tccgagtgga ctcgaaccac cgacccccac catgtcaagg tggtgctcta accaactgag
    10681 ctacggacgc aggatggtgc gttcaattgg actcgaacca acgaccccca ccatgtcaag
    10741 gtggtgctct aaccaactga gctatgaacg caacgttgta ggtgacaacg gggacgaata
    10801 ttagcggcag agtgggaagg tggcaagagg caaaacgtaa ttttctgcgg tatttcgacc
    10861 gtttgcagag cttttaagca aattggctat attttgttga tttgcaaggg tgatttttat
    10921 tcaggatcgc atttacatct gacacacccc aataatccgt gtatcagagt ttaaacagag
    10981 ttgtttgcca ccatctcaaa tctgtcgtgg ataaatcctt cgtagccttt acacttaggt
    11041 agcaataaag gcacacagat gcagcaaagg aataaatcat gggatctgat gcgaaaaact
    11101 tgatgagcga cgggaacgtg caaattgtta agaccggcga ggttattggc gcgacgcaac
    11161 ttactgaagg cgagttgatt gttgaagctg gcggaagagc cgaaaatacc gtggtcacgg
    11221 gggctggctg gttgaaagtg gcaaccggtg ggatagccaa atgcgcacag tacggtaaca
    11281 atggcacgct atcggtcagc gacggtgcca ttgccacaga tattgttcag tccgagggag
    11341 gcgcaattag tctctctact ctcgctacgg ttaatggccg ccatcccgaa ggtgaattca
    11401 gcgttgataa aggttatgcc tgcggtttgt tgctggaaaa cggcggtaac ctgcgtgtac
    11461 tggaaggcca tcgcgcggaa aaaatcattc tcgatcaaga gggcggcctg ttggtcaatg
    11521 ggacaacctc agcggtcgtg gtagatgaag gtggtgaatt gttggtgtat ccaggtgggg
    11581 aagcctgcaa ttgtgagatt aatcagggcg gcgtttttat gctggcgggg aaagccagtg
    11641 atacgttgct tgctggtggc accatgaata atctcggtgg tgaagactct gacactattg
    11701 ttgagaatgg atccatttat cgtctgggga cggatggtat tcagctttac agttccggta
    11761 agacgcaaaa cctgtccgtt aatgtgggtg gtcgggctga agtgcatgct ggtacgctgg
    11821 aaaacgcggt aatacaaggt gggacagtga ttttgttgtc acccaccagc gcggacgaaa
    11881 attttgtcgt agaggaagat cgtgcaccgg ttgaactgac cgggagtgtt gcattactgg
    11941 acggcgcttc aatgattatt ggctatggcg cagagctgca acaatcaacg attactgtac
    12001 agcagggcgg tgtgttgatt ctcgacggca gtacggtaaa aggtgacagt gtcactttca
    12061 gtattggtaa catcaatctc aatggcggaa aactgtggct gatcactgat gcggcaacgc
    12121 aggtgcaact gaaagtgaaa cgcctgcgtg gagagggagc catttgcctg caaaccagtg
    12181 cgaaagaaat ttcacctgac ttcatcaatg tgaaagggga agttaccggt gatatacacg
    12241 ttgagataac agatgccagt cggcaaactc tgtgtaactc tctgaaatta cagccagacc
    12301 aagatggcat cggcgcaacg ctccagcctg cgtaaaaaca caggcgcaac ctgtgaaggt
    12361 tgcgccttgt ctttattagc gggatgctcg ttgcagaatg atggctgacg gcagacgttg
    12421 caggaaccgc atacgcagca tcatcataat ggctgccgac gtcaggccaa taataaagcc
    12481 tatccagaag cctgctggcc ccataggttc aacgaccaga tcggtcagtg ccagaatata
    12541 gccgcttggc aagcccagca cccagtaagc cgtaaaggtg atatagaaaa tggaacgtgt
    12601 atctttataa ccacgcaaaa tcccactgcc aatcacctgg atagagtcag aaatctgata
    12661 taccgccgcc agcagcatca aatgcgcagc cagcgttaca acctcgggat tgtcgttgta
    12721 caacagggcg atttgctctc gcagtgaaac cgtgaatatg gccgtcaggg ttgccataca
    12781 gacacccacc ataagcccgg tccgcgcagc ggtttgcgca tccagcgttg agccttgacc
    12841 cagacgataa cctacgcgga tggttaccgc tgccgccagc gacatgggaa gtacgaacat
    12901 cagtgaacta aagttcaggg caatctgatg tcctgcgaca tcaacaatac cgagcggaga
    12961 cactaacaga gccacgacgg caaacagcgt tacttcaaaa aacagtgcca gcgcaatcgg
    13021 caaaccgagt tgaatcagtc gtttcataac cgcaggatcg ggcttcgcgg tgcctttttc
    13081 gttacgaata tcgcgcatcg agcgggcgcg tttaatgtaa gaaaccatgg caaggaacat
    13141 gacccaatac accgccgctg tcgctacgcc acaaccaacg ccaccgagct ctggcatacc
    13201 gaaatgacca taaataaaga tatagttcac cggaatgttc accagcaggc cgataaagcc
    13261 catcaccata cccggcttgg tttttgccag accttcacac tggttacggg caacctggaa
    13321 gaaaagatag cccggcgcgc cccatagcaa cgcacgcaga taacccacgg ctttgtccgc
    13381 cagagccgga tcgatgtttt ccatggagcg gataatgtag cctgcattcc acagcaccag
    13441 cataatgaga acggaaacaa aacctgccag ccagaaacct tgtcgcacct gatgcgcaat
    13501 gcgctcacgt cgaccggaac catttaattg cgcgataacc ggcgttaatg ccagcagcag
    13561 tccgtgacca aagaggatcg ccggaagcca gatagacgtt ccgatagcga ccgccgccat
    13621 gtcggtggca ctatagccgc ccgccatcac ggtatcgaca aaacccatcg cagtttgggc
    13681 gatttgcgcg agaatcaccg ggattgctaa tgctaataac agacgcgctt cactgatata
    13741 cttctgcacg tgaacacctt ttatttgtag ttatatgaga gactaaaaag ccgcctgaat
    13801 gggcagcaaa aagaagaagc aggggaaatt cagtctattg tagcgaggat ttattaattc
    13861 tccagtgaaa aaatagccat tcttacccct tcactggtaa gggcgttttc cacctgctat
    13921 tgtgctgaac agaatgtctt aactgatttc aggagttgta agtatgttta cggggattgt
    13981 acagggcacc gcaaaactgg tgtcgattga cgagaaacca aattttcgca cgcatgtggt
    14041 ggagttaccc gaccacatgc tggacggact ggaaaccggt gcgtccgtgg cgcataacgg
    14101 ttgctgcctg actgtgacgg aaattaacgg caaccatgtc agttttgacc tgatgaaaga
    14161 aacgttacgc attactaatc ttggtgattt aaaagtgggg gattgggtaa acgttgagcg
    14221 tgcggcgaaa ttcagcgatg aaattggcgg acacttaatg tcaggtcata ttatgaccac
    14281 cgctgaagtg gcgaaaatat taacctcgga aaataatcgt cagatctggt ttaaagtcca
    14341 ggatagtcag ttgatgaaat atatcctgta caaaggattt attggcatcg acggtattag
    14401 cctgaccgtc ggcgaagtca cgccaacgcg tttttgcgtc catttaattc cggaaacact
    14461 ggaacgcacg actcttggga agaaaaaact cggcgcacgc gtcaacattg aaatcgatcc
    14521 acaaactcag gcagtggttg atacggtaga acgtgtgctg gcggcacgag aaaatgccat
    14581 gaatcaacca ggtacagaag cctgatctga aatcgccccc ggagcggggg cgatagaata
    14641 gcctctagcg agcgactcga aagccgtttt cgactccgcg agagaacacc acctgccaga
    14701 gttgaatatc acgggcgcgg aaagcacctg cacaggcatt cagataatag gtaaacatcc
    14761 gtttaaagcg ttcactgtag ttatccgcaa tttctggcca tgcggcgagg aaacgttcat
    14821 accacgccat caacgtagta tcgtaatcag caccgaagtt atgccaatct tccatcacaa
    14881 agtggggttc gctggactga gcaatctggc gtacagaggg caggcaaccg ttcggaaaaa
    14941 tatatttatt aatccaggga tcaacattca gatcggtttt tttcgaaccg atggtatgga
    15001 gcaggaatat accttccggt ttcaaattac gatccaccac cgcaaaatag gtatcgtaat
    15061 ttttcggtcc gacgtgttcg aacatcccca cagaaacaat acggtcaaac tggtcgttca
    15121 ggtcacgata atcttgcagc aaaatggtga catccaggcc ttcacagcgt tcctgagcca
    15181 ttttttgctg ttcggcagaa atggttacgc cgaccacgct tacgtcataa ttagacgcca
    15241 tgtagtgcgc cagtccgccc cagccacagc caatatccag tacgcgcatc cctggtttta
    15301 actgcaattt ttcacaaatc attttgagct tcgcctgctg ggcagattcc agattatcgg
    15361 catctttcca gtaagcacag gaatattgca tgaagggatc aagcatgcgg ctgaacaagt
    15421 cattacccaa atcgtaatgc tctttgccga ctatccaggc acgtttttta ctctgcagat
    15481 tgaagagacg agcgccggca atacgcagcg tgtctttgaa atgatggggg agttggttct
    15541 cgagacctgc gcgtaagact ttgctaaaaa acatatccag tcggtcacat tcccaccagc
    15601 catccatata actttcgcct aaccccaacg atccttcctg cagaacgcgt ttaaaaaaat
    15661 cggggttttt gacacgaata tccgccgggg cagaaccgtt aatggctata ccggcacggc
    15721 taagtaattc gttggcgata cggtaccagt tgtcatccgg tacactgact tcttctatac
    15781 acgatgaact catagtttct ccatcactgg tcggtgatca gaaccttaaa acagcgtaga
    15841 cgctttttgg gctttgtgag aaatctcgca gaaaaaaccg cgagtctata atccgacgta
    15901 gaacagaggg aaaggaggaa cccttgccga aatgaccatc catagtgagt cgggaacgat
    15961 cctgtccccg taataacata atatttatcg tagttttaac aactcgaaat tagtataggc
    16021 cgctaatttc aatcatacaa caaaattttg ttagcagcct aagtataagt atatcgatat
    16081 agatcagtgt gattcgctat gagcgacttc ggcattgcca tgattctggc agccaacttc
    16141 ttcacaacgt tgcatcatgt aacccagagc gaccaacact actgttgata acatcacgct
    16201 ggtggtggtg agcaatggcg tgctgatact gataagccag gaaactacca gacttgcgag
    16261 gaagcacaga cccagttgca gagtgttctg caacgctgcg gcgcgaccgg ttgcatgtgg
    16321 gaagggacgc agcgcctggg cgacaacaat cgggtagatc gcaccgttgg caatcgccat
    16381 cacacagaat gggatcagga tttcgaccag cgacacatgg ctaatgaagc ccgcagccca
    16441 ggtcgcaatg acgctgacag caaacagtac cagcaaccac ggtaataact gcttgccttg
    16501 ccatttctgc agcgcggcac gacagccata accaccaatc agaaacgcga tagtttgcgg
    16561 gacataactt aaaccaataa ctgccggact gtaacccatt tcactaagga tgaacggtga
    16621 accggtcagc catgcaaaaa aactggctga acaggccgcg tatatcagca cgttgccgcg
    16681 ataggtttta gaacgtagca ggtcggtaaa ggtcagacca tcctgactat tgttacgggc
    16741 cttcgtcgtg ggtttgagcc agaaaatagg cagaatcagc accacggtaa tggcaaacag
    16801 ggtggcgaaa atcgcctgcc aggaaaaatg gaccagcagc cagcttccta acagaggagc
    16861 cagtgccgga gacagaccca ccagcggcat gatggtcgca aaaatacggt taactttctg
    16921 tgaaggataa taatctgtca ctaatgcttg ccagataacc gccgcggcgc agacacccac
    16981 agcctgtaca aaacgcaata tcagcagcgt ggcggcgttt tctacccaca gcatccccag
    17041 actacctaac gcaaaaattg tcaggccgat gaataacacc ggtttacgac cataacggtc
    17101 ggagagtggc ccccacagaa gctgggctgc ggcaaaaccg gcaaggaaca gactaaggct
    17161 ggcactgaca gcagacgcag gcgtttgcag gtcggcctgt atggcggcga aagcaggcag
    17221 atacatatcg gttgccagaa aaccgagtac gcttaaacct gccagccaga ctaaaaatct
    17281 tttcccaggt tgcatgtata ttctctaaat ctctcaatta aacaccgccg caaagtgtag
    17341 ggagtgaaat tagtgttgtg aaacggtaat atttgatgat tggtttcaaa aattttgcag
    17401 gcagactatg tggtcagaat attctctgga agttgttgat gcggtagcgc gtaatggtag
    17461 ttttagcgcc gcggcccagg agttgcatcg cgttccttct gcggtcagct ataccgtgcg
    17521 tcagctggaa gagtggctgg cggtgccgct ttttgaacga cgtcaccgtg atgtggaact
    17581 gaccgctgcc ggggcgtggt ttctcaaaga aggacgctct gttgtcaaaa aaatgcagat
    17641 cacccgccag caatgtcagc agatagcgaa cggctggcgc ggtcagttag ctatcgcagt
    17701 ggataatatt gtcaggccag agcgaacacg gcagatgatc gttgattttt atcgccattt
    17761 tgatgatgtc gaacttcttg tctttcagga agtgttcaac ggtgtctggg atgcactttc
    17821 cgacgggcgc gtggaactgg cgattggcgc aacacgtgcg attccggtag gcggtcgtta
    17881 tgccttccgg gatatgggga tgttaagctg gagttgcgtt gttgccagcc accacccgct
    17941 ggcgttgatg gatggcccgt tcagcgatga tacgttgcgc aactggccgt cgttggtgcg
    18001 cgaagacacc tcgcgaacgc tacctaaacg tattacctgg ttgctggata accaaaaaag
    18061 acttgtcgtg cctgactggg aatcatcggc aacctgtatc tctgcaggat tatgcatagg
    18121 gatggtgcca acacatttcg ccaaaccgtg gcttaatgag ggtaagtggg tagcactaga
    18181 actggaaaac cctttcccgg attcggcatg ttgtctgaca tggcagcaaa atgatatgtc
    18241 gcctgcgtta acctggttgc ttgagtatct cggcgatagt gagacgctga ataaggagtg
    18301 gctgcgggag ccggaagaga ctcccgcaac gggtgattaa cgacgatagt cgcggaacgg
    18361 gccgtcagcc acggagcggc gttcaatcaa tcgcggatgc acttcaatgg attgcggttc
    18421 ttcgcgttta ttgacgatac gatccaacag catgttgaac gccgtttcac ccagcgaatc
    18481 ttttggctga tggatcgtgg tcagcgccgg cgtaaaatag cgggcgttgc gcacgttatc
    18541 ataaccgatc agcgaaacat cctgcggcac gcgcagaccc atttcatcag cagcacaaag
    18601 tgcgcccatt gccatgatat cgccaccaca gaagacggca gtagggcgat gcgactgcga
    18661 caggatttgc tgcatggcgc gataaccgga ttcaggttca aagtcaccct gcacaatcca
    18721 gctttccggc actttgatca tcgcttcttc catcgccttc ataaaaccgg caagacggcc
    18781 tgcgccggtg ttacgttcca gcgggccagg gataacgccg atttcgcgat gaccgcgttc
    18841 gatcagataa cgcccggcca tgtagccgcc ttcgaacgcg ttatcaatga ccgcatcggt
    18901 gaagtcagct tttgcttcac cccagtccat caccaccatt gggatatggc gatactcttc
    18961 cagcatcgcc agtaacggct ctgggtactc agaacacatc accagcagac catcgacgcg
    19021 tttttgcgcc atcatcgaca gataagcccg ctgtttctca agattgttcc acgcattgcc
    19081 cagaatcagg gtgtaacctt tctggaagca atttttttcg accgcttcaa tgatctcggc
    19141 aaaataggcc gcttcgctgc tggtcgccag caaaccgata gacttggtgt ggttaacctt
    19201 caggctacgc gccaccgcgc taggggagta gtgtaattct ttgattgctg cccacacggc
    19261 attgcgcgtt tcttcagcga cgaaacgtgt tttgttgatc acgtgtgaca cagttgtagt
    19321 ggaaacgttt gctcgtttcg ctacatcttt tattgttgcc attccatttc actccagacc
    19381 ctaacttcag ctcctgcaaa atcgcaaggt aaacgtttgc ctttacacac cttacgcaca
    19441 acaaaagtgt tgcggtacgc cggaaaaacg acacggagcg tcagaaaggg gtcaatggcc
    19501 agtacgcaaa tagtgtaagc gtggaatttt ggccgatctt gacgaaaagg ggaagtggta
    19561 aaaacactta tcattataaa tgacgggaga ttttttcatc acagtgtgta aaaatgcgta
    19621 atatcacttt ttgtgtggta ataaaaaagg agaaaacttg atgagcaccg accttaaatt
    19681 ttcactggta acaacgatta tcgtcctcgg tttgatcgtg gccgtgggtt tgactgccgc
    19741 gctgcactga tttcctctcg agggagcgcg ttctccctcg catctccttc ctgtttgtta
    19801 gcaaaatggc aaaagtgttt tctctttttg ttattttcat ttttttgtga ttactgtcat
    19861 gctttcgcca ctttaatgaa tatcgcgtaa tgacgcgaag aggtggagat aaggcatgaa
    19921 aattaactat ccgttgctgg cgctggcgat tggcgcgttt ggtatcggga caacggagtt
    19981 ctcgccaatg ggcttgttgc ccgtcattgc gcgcggtgtg gacgtctcga ttcccgctgc
    20041 cggaatgtta atcagtgctt atgcagttgg cgtaatggtt ggcgcgccgc tgatgacgct
    20101 tctactttct catcgtgccc gccgcagtgc gttgattttc ctgatgtcga ttttcacgct
    20161 tggcaacgta ctttccgcca tcgcgccgga ttatatgacc ctgatgcttt cacgcatttt
    20221 gaccagcctg aatcacggag cattttttgg tttgggttca gtcgtggccg caagcgtggt
    20281 gccaaaacat aaacaggcca gcgcagttgc cactatgttt atggggttaa ccctggcaag
    20341 tatcggtggc gtgccggcgg cgacctggtt gggtgaaacc atcggctggc ggatgtcatt
    20401 tctggcaacg gcggggctgg gagtgatttc aatggtaagt ctgttcttct cattacctaa
    20461 aggtggtgca ggggcacgac ctgaagtgaa aaaagagctg gcggtattaa tgcgtccgca
    20521 ggtgctgtct gcattgctga cgacggtact gggagctggt gcaatgttta ccctctacac
    20581 ctatatctct ccggtactgc aaagtattac ccacgccaca ccggtgttcg tcacggcaat
    20641 gctggtgctg attggtgtcg gattttctat cggtaactat ctcggcggca aactggcaga
    20701 tcgttcagtt aacggcacgt tgaaaggctt tttgttgttg ctgatggtga ttatgctggc
    20761 aatcccgttc ctggcccgca atgagttcgg cgcagctatg agcatggtgg tgtggggagc
    20821 tgcaaccttt gcggtcgtac cgccgttaca gatgcgcgtg atgcgtgtcg ccagtgaagc
    20881 gccaggtctg tcttcatcag tcaatattgg tgcctttaat cttggaaatg cgctgggagc
    20941 cgctgctggt ggtgcggtaa tttccgctgg gctgggatac agttttgtgc cggtgatggg
    21001 ggcgattgtc gcgggactgg cattattgct ggtgtttatg tcagccagaa aacaacctga
    21061 agcagtttgc gttgccaaca gctaattaaa caaaaacgca gaaggataac cttctgcgtt
    21121 acagttgctg gcaacgtgcg atttatcttg ccgaatgcgc tgttaacgcc tcatccgtgt
    21181 tggcatggcg cgcgaagcgc atcaggtaat gttgcatttg ccatcagtta ttatgcagcg
    21241 agatttttcg ctacgaattc ccagttcacc agcgcccaga agtgctccag atagccagga
    21301 cgtgcattgc gatagtcgat gtaataagcg tgttcccaga catcaacggt cagcagcgga
    21361 gtcgcatcgg tggtcagtgg agtacccgcg ttagaggttg aaacgatagc cagtttgcca
    21421 tcgctgtttt tcaccagcca ggtccagcca gaaccaaagt ttttgatcgc tgcatcagta
    21481 aactgagctt tgaaatcggc aaagctgcca aaagatgcgg cgatagcttc agcgactttt
    21541 ccagtcggtt cgccacccgc gttcggtgcc aggcagttcc agtagaaagt atggttccag
    21601 acctgagctg cgttgttgaa tacgccacct tcagagctgc gaataatctc ttccagtgat
    21661 ttaccttcaa acgcggtacc tttgatcagg ttgttcaggt tagtgacata agtctgatga
    21721 tgcttgccgt agtgatactc gatggtttcc gcagaaatgt gcggtgccag agcatcttta
    21781 gcatatggta gtgcaggtaa ttcgaatgac attgctactc tcctttatta ttaatttgca
    21841 tacgtacaat agccttattg tgcgtatgga tagggtagca aattaaaagg tgagacaaaa
    21901 gatgaactta ccctgttgcc gtaacaacag ggtaaagttt tagcgaagtg tttttggggt
    21961 cattacccgg cgagcgccaa catagtggcg ctgccagtag tcttcactga gagaagtgat
    22021 ttgaatttct tggcccgtgc gcggtgactg aataaatttg ccgttgccga catacacgcc
    22081 gacatgatcg gctgtaccgc gtccctgagt acggaagaag accaggtcgc cgtttttcag
    22141 ttcactacgt tcgattggcg ctgcatcgcg caggtgatac atttcattcg ccgtacgcgg
    22201 aatacgaatt ttcaccaaat ctttataagc gtaataaacc agaccgctgc aatcaaaacc
    22261 ggtacgcggt gaactgccac cccaacgata tggcttacca atttgctgca tcagtttatt
    22321 cattgccact ttcgtggctt tttgcacctt cgctttgtgc gcgtcggcaa gggttcctga
    22381 cgcggcattt ttgactttca cacaatgcga tttatagccc ttacgtttgg tacacttttc
    22441 ggtaaagttg gctgaggccg ttttattgac ggtgtgtttg ctgcgagttt tggcgttttt
    22501 agaagcggta atggaagatt ttttaacgga agaagcggtt ttgcttttgg tcgtactttt
    22561 actggcggct tttttcgctg ttttctgggt ctttttggtg gttgccgtgc ttttcttttt
    22621 atctgctttt ttggtgatat gagtagtagc agaactctcc ctggcttgct ttgaagcatg
    22681 ggccataggc gttaaaggca gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg
    22741 gtttatccgc gccactgagc gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat
    22801 ttacaaaact tgatgattct aatgcataga agaagagaaa gttaatagct tttattgtta
    22861 aaaaatacgt ttcgtcggcg agcgcaaaaa aatgcaacag tgaaagctaa aaatggtcat
    22921 aaatacacca gtttgccgtg cataagggaa gaaacgaata tttgaaatgc aacaattaga
    22981 gcaaagggat aaaatagcaa aagcgtgatg aaaatgttat ttcccgatgc cgcactgacc
    23041 cgctacaatg tcagattact gccgtaaaga agtttgagga agcaagacaa tgagcaccac
    23101 tatcgaaaaa atccaacgcc agattgctga aaacccgatc ctgctgtaca tgaaaggttc
    23161 accgaaactg ccgagctgcg gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg
    23221 cgaacgtttt gcctatgttg atattctgca gaatccggac attcgtgcgg aactgccgaa
    23281 atatgcgaac tggccgacct tcccgcaact gtgggttgac ggcgagctgg tgggcggttg
    23341 tgatatcgtg atcgaaatgt atcagcgtgg tgaactgcag cagctgatca aagaaactgc
    23401 cgctaaatac aagtctgaag agccggacgc ggaataatcc gtcctgcaat actgacggat
    23461 gcgaacgggc atccgtcagt cctttctcta tccccaatcc agcccttgtg gcgaacttga
    23521 aaatcccgcc tcacgtaacc gcgtaaacat cggcgtttgc cggaccggta ggtcattcac
    23581 ttctgttagc gtaaagcgca gccgtggttc gcgactggac agctgtcggc tgtttaaagg
    23641 cgcgaagaaa ctagtcgcgg gtcgccggtg aaaacacgtc cggcaggggc gatgaagggt
    23701 ttagattatc tgccattaac gctattcccc gtgaatatca tgcatgatgc gtggaatagc
    23761 ctcactatag atgcctgaat cattgcatgt catcaggcat cgactcgatt acacctcttc
    23821 ggcggcaggt agcggccagc ctcccagacg tttccagcgg ttgacgattt cacaaaacag
    23881 cacagcagtg cgttcggtgt cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat
    23941 gccagcggtc tggcaagcct ttgacaatac ggtttgtccg agtgccagcc cggccagtgc
    24001 agcagtgtca aaagtggcga aagggtggaa cgggttacgt ttcagtgagg cgcgttctgc
    24061 ggcggccatc ataaagctgt gatcgaaatt ggcgttgtgc gccaccataa tggcgcggtt
    24121 acaaccgctc gctttaatac ctttacgcac aactttaaaa atttcgtgca gcgcctcgta
    24181 ttcgctgacc gcgccgcgat cgggatcgtt cgggtcaatg ccgttgaagg cgagggcctc
    24241 tggctgcaaa tttgcgccga caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat
    24301 cagccagcct tgttcatcca ttttcagggt gatggcggca atctcaagca gcgcatcggt
    24361 tttggcgtta aatccggctg tttcaacatc gatcacaaca ggataaaaac cacgaaaacg
    24421 gtcgcacaga ccggtaagtt gagcgttatc ggacatctga ttctcttaat acggataaat
    24481 tgcagcgcgc attatgacaa atattgcttg tcgatgcagt aaagatgcgg gcgcgatgag
    24541 ttcacgcccg gcaggagatt agttgcccag accgcgaccg gcgtctttct cttcgattaa
    24601 ttcaattttg taaccgtccg gatcttccac aaatgcgata accgtagtac cgccttttac
    24661 cggacccgct tcacgggtta cgttaccccc gttttgacgg attttttcgc acgcttcagc
    24721 ggcgttatct acgctaagcg cgatgtgacc ataagcagtg ccgagttcgt atttatccac
    24781 gccccagttg taggtcagtt caatcactgc ttcttcggtt tccgggccgt aaccaacaaa
    24841 cgccagtgag tatttgtatt ccgggttttc gctggtacgc agcagtttca tgcccagcac
    24901 gttggtataa aaatcgatgg agcgttgcag atcgccaacg cgcagcatgg tatgaagaag
    24961 acgcatcttt atatcctcaa aatggctaac agattcaaaa agttgtttta gtatagcggc
    25021 ggctttgcgc cgctcgcaat gttggattac agcgtcgggt aatcagtgta accttcagca
    25081 ccgccaccat aaaaactttc ggcacgctgc gggttaagct cagctttgcg ctgcaagcgg
    25141 gcgaccagat ccgggttcgc aatccagtca cgcccaaatg ctaccgcatc aattaaccct
    25201 ttgccgatca gcgtttcagc tttttctact gtgtatgcac ctgcgccgat aatcggaccg
    25261 tggaaacggg cgcgtacttt ttcgcgaaac gcatcagtat atggttcacc tcccgcccaa
    25321 tctggttctg acatatgaag ataagcaatg ccgcgtttac ccagttgttc aatcagatac
    25381 agtgcgtcgg cttcttcatt cgggccgtta tccgtgttct ggaaagtacc gattggcgaa
    25441 atgcgaatgc caatgcgatc ggcaccccat tcttcaatcc cggcatcgac cacttccagc
    25501 accagacgcg cgcgattttc cacgctgccg ccgtactgat cggtacgatg gtttgaagaa
    25561 ggggaaagga actgatgcag caaataaccg tgagcagagt ggagctctac cagatcaaaa
    25621 ccggcttcac gcgcgttagc aatggcctga cggaaatcat tgacgatacc cggaatctct
    25681 cccaattcaa gcgcacgcgg tatggatgtt tcaacacgga tcgcctgacc attttcatcg
    25741 cgcagagaag tacgtgttcc cgcgctaagt gctgaaggcg ctaccggtgc ctgaccgcca
    25801 ggttgcaggc tggcgtgaga aatgcgtccg gtgtgccaca gctgtacggc catatgacca
    25861 ttttcagcat gaacgccagc ggtaattttt ttccatgcgg caatttgttc cgggctatgg
    25921 atgccaggtg cacctgcgta tccttttgcc tgggcagaaa tttgcgtggc ttcgctaata
    25981 atcaaaccgg cactggcacg ttggcgatag tattccgcca tcaacggggt agggatgtca
    26041 cccggttcaa tactgcgcag acgcgtcagc ggtgccataa aaatacgatt tgccgccgtg
    26101 atcgcgccca ctttcagtgg ggaatacagt ttttcagatg acataaaggc tcccggtcgt
    26161 ctggtgataa aaaatgcctg ctaaacggca ggcgtcgcaa taatgttttt tacatgggcc
    26221 agcgcgtttt ccagtggctc gaaactgcgc gaaattttgg cctgcagatt cgcgccaagc
    26281 cacagtgcgt aaagcacttg tgcctgttgc agcggttcgc cacaaaaggt taaacaatgg
    26341 ttctcacggc cattttccag cgcctgagag agcagggcga tcacgccgcg agccccttta
    26401 tccattgcgc tgcgcatatc ttctgacaga tcgcacactt cggcagagag ttttactgtc
    26461 aggcaaccac tgatggttcc atgttggcaa aactggttca gtgtttgctg gtaataagcc
    26521 agtatgcggt cgcggtagtt accttcgccg gattgcagca actcagtcag tcgctggtga
    26581 tatgcggcgt aatggcgctc aagcatagca acgccaaacg cttctttaga acgaaagtag
    26641 tgatagaagg accctttcgg cacttcagcg gtttttagga gttcgcttag ccccatcccg
    26701 gtgaatccac gttgcaggca aagttgctcg cccgtcgcca ggagatgttc gcgagtatca
    26761 tgttcggtgt gtttgttcat gcgttggagt ttagtagacc agtcggtcta gtgcaagcaa
    26821 agttgctact ttggacatta acagcttcaa taatggtaat aatcttatct gaggagggcg
    26881 aggtgacgga gcaattagag ttctttcccg tccagagccc gtgccgggga atttgccagt
    26941 ctgacgaacg gggattttgt cgcggctgtt ttcgtagccg ggatgagcgt tttaactgga
    27001 ataagatgag cgatggcgaa aaacaagaag tgttgcgcct ttgtcggcag cgattaatgc
    27061 gcaaattacg tgtaaataag ccagcgccat cagacgaacc ggagcaacca tcactctttt
    27121 gagcctaatc ttgcgtatac tcctctcata tctgataaga ggaagcgatt atggttcagc
    27181 gtattactat tgcgccgcaa ggcccggagt tttcccgttt tgtgatgggc tactggcgat
    27241 tgatggactg gaacatgtcc gcccgccagt tggtcagttt tattgaagag catctggatc
    27301 tcggcgtgac caccgtggac catgctgata tttatggtgg ctatcagtgc gaagcggcgt
    27361 ttggcgaggc gttgaaactg gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat
    27421 gcggtatcgc gacgaccgcg cgtgaagaaa acgtcattgg ccattacatc actgaccgcg
    27481 atcacatcat taagagcgcc gaacagtcgc taattaatct cgcgaccgat catctggatc
    27541 tgctgttaat ccaccgacca gacccgttaa tggatgccga tgaagtggcg gacgccttta
    27601 aacatctgca tcagagcggc aaagtgcgtc attttggtgt atcgaacttt acacctgcgc
    27661 aatttgcgct gttgcaatca cgcctgccat ttactcttgc cactaatcag gtggaaatat
    27721 ccccggtgca tcagccgtta ctgctggatg gcacgctcga ccaactgcaa caactgcgta
    27781 ttcgtccgat ggcgtggtcc tgccttggtg gtggtcgtct gtttaatgat gattatttcc
    27841 agccgctgcg tgatgaactg gctgtggtgg cagaggagtt aaacgcgggc tcgattgaac
    27901 aggtggttta cgcctgggta ctacgtttac cgtcgcagcc gctgccaatt atcggctcag
    27961 gtaaaattga gcgcgtacgg gcagctgtcg aagcagaaac actgaaaatg acccgtcaac
    28021 aatggttccg tatccgtaaa gcggcactag gttacgacgt accgtaagca cttttaggaa
    28081 tagccgccgt tcaaaaatgt gtcactggtt tacacttatt caggaatgca caatgaacgg
    28141 aggtcctatg aaacgtttta gtctggccat tctggcgctg gttgttgcga ccggcgcaca
    28201 agctgccagt gaaaaagtcg agatgaacct cgtcacgtcg caaggggttg ggcagtcaat
    28261 tggtagcgtc accattactg aaaccgataa aggtctggag ttttcgcccg atctgaaagc
    28321 attacctccc ggtgaacatg gcttccatat tcatgccaaa ggaagctgcc agccagccac
    28381 caaagatggc aaagccagcg ccgcagaatc cgcaggcggg catcttgatc cacaaaatac
    28441 cggtaaacat gaagggccag aaggtgccgg gcatttaggc gatctgcctg cactggtagt
    28501 caataatgac ggcaaggcga ccgatgccgt catcgcgcct cgtctgaaat cactggatga
    28561 agtcaaagac aaagcgctga tagtccacgt tggcggcgat aatatgtccg atcaacccaa
    28621 accgctgggc ggtggcggtg aacgctatgc ctgtggtgta attaagtaat taagaagcca
    28681 gcgtaccttg cggcggtgct tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa
    28741 ttgccgccag ttctcgggcg gcaggttgat gatgacgggc aaggctgtcg caaatccgct
    28801 gtaattcttc aagtgtggcc gccagtgatt tttgctgaac gccacgctca ctcatcactc
    28861 cccgcaacag tgaaatacag ttatcccgta ctcgcgataa cggatcggaa cgcgattccc
    28921 agtcgcgcaa ttgccagaca acatgagaac agttcagcaa cactacaccc cagcgtaata
    28981 accaacggcg agccagcgca tcctggctgt tactcaactg actgacgtga tgataagtga
    29041 gcgattcaaa ttcgctttca ctcagtgttg gatggcggct tagttgatcg acaaaatccc
    29101 ggcgcagcgc gcgaatatgg cggcggcttt tacgagcatc cgatcctgga cgcagaatgg
    29161 cgaacgctaa ccacgccaac gcgacgccaa caatttttgc cagattatcg ttaagaaaat
    29221 cagcaaaatc atacaccggg ggattagtga cagcgataaa cgaacccata aaaacaatca
    29281 gttgccccca cagtgcggca aatttgggca tctgcaattt aagaagctgc attgtcgcca
    29341 gcagtggaaa gagaaacagt aaaaattgcc aaagatcgct aatctgcacc atcaggccaa
    29401 atttgaccac aaagctgaat agcgaaagta acaccagcgt gcgcatcagc agtgacaacg
    29461 acttaaacgg tgctgcgacg gcggagtaga gtacgcagct aattgctgcc agcgttaaag
    29521 cattggcacc ggcatcccat tgcgaagcaa tactccatgc gccaatcatc atcaacgtac
    29581 aaaatgtacg cagcccgctc cacatagctt cggcgttatc ggtatgacga gccaggccgg
    29641 atgtccgtgg gagtctggca tgatcatcta caccgctttg cagacgatgt aattcctgac
    29701 tactttgcag ataaaggcgg caaaaataac gtagtcgctg ccagaaggcg acgtgccggt
    29761 agtcggcgac gttggtcggg cgtagcgggg agataatacg tgcgatggtg taaacatctg
    29821 tttgcgaact ggcgagcgcc gtcagcagct gttcgagaat ttctcgcgtg gtatctggcg
    29881 gtgagggcca gttgagcaac atacgtcgta ggctggagat gacactggtc atacgtaatt
    29941 gctggtggag cagcgcatta aggcgtgcgt tttgctggcg aaaacgatag tggctccaga
    30001 aagcctggat acgcagcaaa ttcatggtca gtatctgccc aatcacccct tcatgtgcag
    30061 cacgaatggc atcggttgtt tcaggctgcc agagtaaact ggcatgttcc agtaatcggg
    30121 cgtgcatgtt tttcaatgcg gttaaaaggg ctgtagcatc ggaactgctc ggcaggatca
    30181 tcatcatcat gccgccgcac aaaataccga ctatcacctc gcaaacacgc gcctgagcga
    30241 tatcccacag ctggctggcc tcagtaatat taaccatcgg aaaggcgatg atggcagccg
    30301 tgtagcccgc cagttgaaat gcatacgcga cgttattcgt gaagtgcgca caggcccagg
    30361 tacaaaagcc aagccacgcc gacatgctca atagaaaaaa ccacggctca ttgagcgtat
    30421 gtccggcaag aagcagtgcc gcaatggctc cgagcaaact gccagcgatg cgtccgaggc
    30481 ttttgctgat gacaccgcca acggtgggaa agctaaccac cgcagccgag gtcatcgccc
    30541 aatagggctc atcaagattc aaataatagg caaccgtcag cgccagacac atggcaatgg
    30601 tattgcgtaa cgcataacgc cattgcgcca gcgtggccct gaaccagggc aaattgcgca
    30661 aggaccatga cgatgcgttc attattgacc tacggcaatg ctacaggtgg tgcccgacac
    30721 cagcgtgaca tcgcctggga ctttatccag cgtaaagcgg acgggaacgc gttgggcgag
    30781 gcgaacccag gggacgttag gtttcacatc cggaataagg cttgagtcac tttcaacgct
    30841 ctgatcataa atcgcccgac cgatactgga cacgtgaccc tgtaacgttt tgttatcact
    30901 atagagggta atttgtgctg gtgcaccttc gcggatatgg cgcaacttgg tttcttcaaa
    30961 ataaccaatg acgtaaaacg agtggctgtc aacaagggca aacagcggtt tcccggtgtc
    31021 agcgtagtcg ccgatgcggg tagtgagatt agtcacccat ccactcaccg gggcgcgaat
    31081 ttccatttgc gccagttgcc attgtgcctg tttcagcgtc gcctgtgcgg catcaacgct
    31141 ggcctgcatc gctttaacat tgaggttagc ggtatcgagt tcttcggcag aaataaaatt
    31201 ttgtgagaga tgacgacgac gattcgcttc gttattggct tttgcgaggt cagattgcgc
    31261 tttcgccagc tgagcctgtg cgttcagctc ggcgatctga aagggcgttt tatcgatcgt
    31321 gagcagaaga tcccccgcgt tagccagctg gttgtctttt atattcagct caacaatacg
    31381 tcccgacacc tgtggggtga tagaaacctg ttctgcgcgt attttcccgt cgcgtgtcca
    31441 cggagattgc atgtaataat tccacagcca ccatccggca agaaccgcaa ctaccgctac
    31501 aatgattgtt gagaaatact taattgtttt aattgacata gttaccacgc aatcagtatt
    31561 gccagagcaa ggcaaacgca aatcgcaaac agcgataaat ccattaacaa gggatgccag
    31621 atgtcaccag cgtagatcca gccacgaagc aggcggtgta tgacaagcca gatgacgaat
    31681 ccaaacgcga aggctttgaa aaacggagga aagtagacgg acgcaccgaa caccagatct
    31741 tgtaagggca atcctgttgc attgagcata aacttcacaa agtagattcc tttacgacag
    31801 aatatattgc gttagattaa taaatattct ttaagtgcga aaaatttacg cgcaatttct
    31861 gaaaaatatg tctgacggta accaaatgca gcaatacatt tgttttagca atacaattgc
    31921 tgcacactat tctaaaagcc gcataatatc ttagcaagct aattataagg agatgaaatt
    31981 ggaatcgcca ctaggttctg atctggcacg gttggtgcgc atatggcgtg ctctgataga
    32041 ccatcgcctg aaaccgctgg agttaacaca aacccattgg gttacgttac acaatatcca
    32101 tcagttacct ccagaccagt cgcaaattca actggcaaaa gcgattggca tcgagcagcc
    32161 atcactggtc cgtacgctgg accaactgga agaaaaaggg ttaatttcgc gtcaaacttg
    32221 tgccagcgat cgtcgggcta aacgtattaa actgacggaa aaggcagagc cactgatcag
    32281 cgaaatggag gctgttatta acaaaacccg cgcggaaatt ttacatggca tctccgcaga
    32341 ggaactggaa caactgatta agctcatcgc aaaacttgag cataatatca ttgagttaca
    32401 ggccaaaggg tgaaatgaag ggggcgtgtg gtcagtaacc tgaccacacg caaacttatt
    32461 tagcgcgggg aaacggtcac ctgactgcca ttgctggcca gtacgacacg ttggcctgga
    32521 gagaaatgag tgttcccttg tttctgtacc accatgatgg tgttgccatc atctttacga
    32581 atttccagct cgacaccctg cgttttgttc attgcactct gtacgccctg accggctaca
    32641 ccacctgcaa cagcccctgc tgcagtagcc agagaacgcc cggttccgcc gccaatagta
    32701 tttcccagga aaccaccaag tacagcacca ccaattgcgc cgataacgtt ggaatcatca
    32761 ccgccctgaa tctgtacctg acgtacatta acgatggtgc catagctgac attctgtact
    32821 tgtttcgctt cagaagcggt ataaacatcc cctgacaggg tgtcgttatt aacacaaccg
    32881 acaagagaca gacctaccat tgaaacaacc aatacgcgtt taatcattga aaaatctcct
    32941 gttcaccatg aaacgccacg caagcatccc tcatggtcaa agtatatggc atatgtgaat
    33001 ccatggttac atgttctacc aaatatgagc aaatcataat gaaagtagga tcaaccaatt
    33061 ataaacaaaa tattcatgat gattattgat ttgaagataa tgttttacta ccaacggtaa
    33121 aaattggtca ttccgcatgg tattcacgaa aagtttatgt ttgagtggtg cgcaaacgtc
    33181 agattgaaaa aggatgaact atgaaatcgg gccgctttat tggcgttatg tcaggcacca
    33241 gccttgatgg tgttgatgtt gtgttggcga caattgatga acaccgggtc gcacagctgg
    33301 caagtttgag ctggccgatc ccggtgtctc tgaaacaggc tgtactggat atttgccagg
    33361 gccagcagct tacactttcg cagtttggac agcttgatac tcaacttggg cgactttttg
    33421 ctgatgcagt caatgccttg cttaaggaac aaaacctgca ggcgagagat atagttgcga
    33481 tcggctgtca cggtcaaacc gtctggcacg aaccgacggg cgtggcacca cacactttac
    33541 agattggcga taacaatcaa attgtggcgc gcaccggaat tacggttgtc ggtgattttc
    33601 gccgtcgcga tattgccttg ggaggacaag gtgcaccgct ggtacctgcg ttccatcatg
    33661 cgctgctggc tcacccaacc gagcgacgaa tggtgctcaa tattggcggc atcgccaatc
    33721 tgtcactgct cattcctggc cagccggttg ggggctacga taccggtcct ggtaacatgc
    33781 tgatggatgc ctggatctgg cgtcaggccg gtaaacctta cgataaagat gccgagtggg
    33841 cacgggcggg taaagttatt ctcccactgc tgcaaaatat gctcagcgac ccatatttct
    33901 cgcaacccgc accgaaaagc accggacgcg aatactttaa ctacggctgg ctggagcgcc
    33961 atttgcgcca ttttccgggg gttgatcccc gtgatgtgca ggccacagtg gtagaactca
    34021 ccgccgtgac catttctgaa caagttttgt tgagcggtgg ctgcgaacga ttgatggtat
    34081 gtggtggagg tagtcgtaat ccgctactca tggcgcgtct ggcggcatta ctgccaggca
    34141 cagaagtcac caccaccgat gccgttggca ttagtggcga tgacatggaa gcattggcgt
    34201 tcgcctggct tgcctggcga acgctggcgg gattaccagg aaatctgcct tccgtcactg
    34261 gcgcaagaca ggagacggta ctgggggcta ttttccccgc taacccgtga tatcagagtt
    34321 aactgaattt ttcttaccgc gtctgccgtt acacttggaa cgttagggag ggcgtattgc
    34381 cctccagacc aggaaagtct tcgggatatg tctatgaaaa aactgttact gatctgtttg
    34441 ccggtgcttc tcaccggctg tagctcgttc aatcaactgg ttgagcgcat gcaaaccgat
    34501 acgctggaat accagtgtga tgaaaaaccg ttgacggtca aactgaataa tccgcgccag
    34561 gaagtcagtt ttgtttacga taatcaacta ctgcatctca aacagggcat ttcagcgtct
    34621 ggtgcacgtt acaccgacgg aatctatgtt ttctggtcga aaggcgagga agcgactgtc
    34681 tataaacgcg accgcatcgt cttgaataac tgtcagttac aaaatccgca gcgttgagat
    34741 ttttccaggg gcggcgcaca atagcgtcac ccactgacaa tccgtaaaga aaaccatgtc
    34801 tgataacgac gaattgcagc aaatcgcgca tctgcgccgt gaatacacca aaggcgggtt
    34861 acgccgccgc gatctacccg ccgatccatt aacccttttt gaacgttggc tctctcaggc
    34921 ttgtgaagcc aaactggctg accctaccgc gatggtggtc gctaccgtgg atgaacatgg
    34981 tcagccttat cagcgcatcg ttttactcaa acattacgac gaaaaaggca tggtgtttta
    35041 caccaacctc ggcagccgta aagcacatca aatcgaaaat aatccgcgcg ttagcctgct
    35101 gttcccgtgg catacccttg aacgccaggt gatggtgatc ggtaaagcgg aacgactttc
    35161 gactctcgaa gtgatgaaat attttcatag ccgcccgcgt gatagccaga ttggtgcatg
    35221 ggtttcgaag cagtccagtc gcatttctgc ccgcggtatc cttgaaagta aattcctgga
    35281 gctgaagcag aagtttcaac agggcgaagt gccattgcca agtttttggg gcggttttcg
    35341 cgtcagcctt gaacagattg agttctggca gggcggtgag catcgcctgc atgaccgctt
    35401 tttgtaccag cgtgaaaatg atgcgtggaa gattgatcgt cttgcaccct gaaaagatgc
    35461 aaaaatcttg ctttaatcgc tggtactcct gattctggca ctttattcta tgtctctttc
    35521 gcatctggcg aaaagtcgtg taccggcaaa ggtgcagtcg ttatatacat ggagattttg
    35581 atggcaagca gtaacttgat taaacaattg caagagcggg ggctggtagc ccaggtgacg
    35641 gacgaggaag cgttagcaga gcgactggcg caaggcccga tcgcgctcta ttgcggcttc
    35701 gatcctaccg ctgacagctt gcatttgggg catcttgttc cattgttatg cctgaaacgc
    35761 ttccagcagg cgggccacaa gccggttgcg ctggtaggcg gcgcgacggg tctgattggc
    35821 gacccgagct tcaaagctgc cgagcgtaag ctgaacactg aagaaactgt tcaggagtgg
    35881 gtggacaaaa tccgcaagca ggttgccccg ttcctcgatt tcgactgtgg agaaaactct
    35941 gctatcgcgg cgaacaacta tgactggttc ggcaatatga atgtgctgac ctttctgcgc
    36001 gatattggca aacacttctc cgttaaccag atgatcaaca aagaagcggt taagcagcgt
    36061 ctcaaccgtg aagatcaggg gatttcgttc actgagtttt cctacaacct gttgcagggt
    36121 tatgacttcg cctgtctgaa caaacagtac ggtgtggtgc tgcaaatcgg tggttctgac
    36181 cagtggggta acatcacttc tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg
    36241 tttggcttga ccgttccgtt gatcaccaaa gcagacggca ccaaatttgg taaaactgaa
    36301 ggcggcgcag tctggttgga tccgaagaaa accagcccgt acaaattcta ccagttctgg
    36361 atcaacactg cggatgccga cgtttaccgc ttcctgaaat tcttcacctt catgagcatt
    36421 gaagagatca acgccctgga agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag
    36481 tatgtgcttg cagaacaggt gacacgtctg gtgcatggtg aagacggttt gcaggcggca
    36541 aaacgtatta ccgaatgcct gttcagcggt tccttgagtg cgctgagtga agcggacttc
    36601 gaacagctgg cgcaggacgg cgtaccgatg gttgagatgg aaaagggcgc agacctgatg
    36661 caggcgctgg tcgattctga actgcaacct tcccgtggtc aggcgcgtaa aactatcgcc
    36721 tccaatgcca tcaccattaa cggtgaaaaa cagtccgatc ctgaatactt ctttaaagaa
    36781 gaagatcgtc tgtttggtcg ttttacctta ctgcgtcgtg gcaaaaagaa ttactgtctg
    36841 atttgctgga aataatgcat taagtggaaa gggggagtga gaaatcactc cccctggttt
    36901 ttatacaggg aacatgatga aaaatattct cgctatccag tctcacgttg tttatggtca
    36961 tgcgggtaac agtgcggcag agtttccgat gcgccgcctg ggcgcgaacg tctggccgct
    37021 gaacaccgtt caattttcta atcacaccca atacggcaaa tggactggct gcgtgatgcc
    37081 gcccagccat ttaaccgaaa ttgtgcaagg cattgccgcc attgataaat tacacacctg
    37141 tgatgccgta ttaagtggct atctgggatc ggcggagcag ggtgaacata tcctcggtat
    37201 cgttcgtcag gtgaaagcag cgaatccaca ggcgaaatat ttttgcgatc cggtaatggg
    37261 tcatccggaa aaaggctgta tcgttgcacc gggtgtcgca gagtttcatg tgcggcacgg
    37321 tttgcctgcc agcgatatca ttgcgccgaa tctggttgag ctggaaatac tctgtgagca
    37381 tccggtaaag aacgtcgaag aagcggttct ggcagcgcgc gaactcattg cgcaagggcc
    37441 acaaattgtg ttggttaaac acctggcgcg agctggctac agccgtgacc gttttgaaat
    37501 gctgctagtc accgccgatg aagcctggca tatcagccgt ccgctggtag attttggtat
    37561 gcgccagccg gtaggtgttg gtgatgtgac gagcggttta ctgctggtga aactgcttca
    37621 gggggcaacg ctgcaggagg ccctggaaca tgtgaccgct gcagtatacg aaatcatggt
    37681 gaccacaaaa gcaatgcagg aatatgagct gcaagtggtt gctgctcagg atcgtattgc
    37741 caatccagaa cattatttca gcgcaacaaa gctctgaaat atctcatttc ggcccacaac
    37801 gacctgtggg ccgatggcta ttactttaag ccttccgctg acagcgcgtc ttgtacttcc
    37861 ggacgttcag ccatacgttg cataaatgcc gcaatgtgct ctaacccttc cagattcagt
    37921 ttcaccgcgt atgcccagcg cagaaccgta aacagatagg catcagcaat tgtaaatcgt
    37981 tgcccgcaga tccagtgctc atccttcagt gcctcgttca catattgcag cttcttctcc
    38041 agctgcgcgc gaactgtcga tttgtactct tccggtgtat cagggcgaaa cagaggtgtg
    38101 aaacctttat gcagctcggt ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa
    38161 atactgttta ccggtgccag caactggcgg tcggggacgc tgtcggcaag atactgcata
    38221 atcgctacgc cttccgtcag caaagtacca tcgtccagca gcaatgcagg tacctgtccc
    38281 ttagggttaa cggcaaagta atcgtcaccg ttttcgagac gttttttcat taaatccaca
    38341 ctgacgagcg taaaatcctt tccgctctca cgcagggtga tatgggaagc gagagagcag
    38401 gcgcccggtt tgtagaacaa tttcatcggt aactcctttt gtctgtggtt taaagtatgg
    38461 tagtgcgttg ctgaacaaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt
    38521 ttccctgaat tttacgctac ggctgctttc gccgctttgt ctgcagcgtc atcctgcgtc
    38581 atgcggtgca gtttcggcgc ggtaaacagc atcagtactg caataacggc agtagcgaca
    38641 ccaatctgca agaatacgcg accatagact tccagtgaca tcagcggatc ggtaacgtta
    38701 tccggcacag ccatcatacc tgcaacataa ccaccaatca ggtttgcacc ggcagtggtc
    38761 aggaaccagc tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt
    38821 gccagaccca ggccagagat catcagttcc ccgatactct gcaggccata gctcgcgacc
    38881 agccagctta cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc
    38941 aggaacgcac cagaacacat caccatgccg atagcaaact tggttggcat tggcagggta
    39001 tcgcccatct tgttatagat agcggccaga atcgggctac caatgatgat ccagaacggg
    39061 ttcagtgcct gatactgctc aggttctacg gccaggccca gaatggtgtg ttcaacgtta
    39121 cgaatcgcaa agaagttcag tgacgttggc atctggctat aaagcacgaa gaagataatg
    39181 gcttcgagca tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg
    39241 aaggcttctt taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc
    39301 gcaacttcct gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata
    39361 atggtcagca gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt
    39421 ttaacccaac gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc
    39481 aacgcaaacg caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag
    39541 aagaaagagc cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg
    39601 tcgtttttct catagcatgt agaaagcaga gaagacgggt tagctttaaa caggccgtta
    39661 ccgaccgcaa tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca
    39721 accagagcat aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc
    39781 agtaccttgt cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag
    39841 gaagagaaaa gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca
    39901 gccataattc cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg
    39961 agatagaacg ccttcggttg tttaaaagcg ttcaaactga cgctttcagt tggtttttgg
    40021 tttgcagtgg acacttttac ctcttttttt acatcccata ttaacggggg tgctttctcg
    40081 cgttatgtct aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg
    40141 ttcactatct tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc
    40201 ggggaattca accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaacg
    40261 gcagattttt tcactaccct gagagggtga tgatggctta atggcgatat gttctactat
    40321 ttggcgtttt tcctagtgag atagtccagt ttctgaaaaa tagccagtgt aatgttttgt
    40381 aggtcaatat ttgttgcgtg tgtaaattta aatgatttta acctgattct tgcaatgttt
    40441 ttacattgcc acagcatcgt gatcttgatc acgaatgaat agaaaatttc gctattaaaa
    40501 aaacaattaa cctgcatttt tggttatctg caaacggatt acctgatgat aacgctaagc
    40561 aatggcatca ttgacgttaa aaagatgttt taaaagagga taaagaaagg ttatgaatgg
    40621 ggtaatcggt gttacccctg atctttagat gtcaactttc tctttgtatt cacaaagatc
    40681 ttcaataata caagagccac agcgaggctt gcgggcaatg caggtataac gcccgtgcag
    40741 gatcaaccaa tggtggcaat cgactttaaa ctctgccgga accactttca gtagcttttc
    40801 ttctacctgt tcgacgtttt tccccggcgc aaattgagta cgattgcaaa cgcggaaaat
    40861 gtgcgtgtcg acagcaatag tcggccagcc gaatgcagtg tttaatacga cgttggctgt
    40921 tttacgacct acaccaggca gggcttcaag cgcagcacga tcttccggaa cctcgccatt
    40981 atgttgttcc agtaagatac ggcaggtttt gatgatattt tctgctttgc tgttataaag
    41041 gccaatcgtt ttgatatagg ttttcacccc ttcaacgccc agttcaagca tcgctgcagg
    41101 cgtattagcc accgggtaga gtttcgccgt cgccttatta acactgacat cggtcgcctg
    41161 agcggaaagc agtacggcaa tcagcaattc aaaaggcgaa ctgaaattaa gctcggtggt
    41221 gggatgagga ttgttctcac gcaggcgagt gaggatctcc aggcgttttg ctttgttcat
    41281 cagacattcc ctgtttcacc gtttggcaat gcacgttcgg cagctgcttc agtacggcgc
    41341 tttttcattt tttcatcaat caggtatttt cctgccagca tcagtcccag gccaataaac
    41401 gcacctggtg gcagcatcgc cagcaggaaa ggggagtcgg tgtggaaaat ctccacgcgt
    41461 aatacttttg cccagctacc taacagcgca tctgcaccgt caaacaacgt gccattgccg
    41521 ataatttcgc gtagtgaacc cagcacgaac atggcgcagg ttgcgcccat accaattgaa
    41581 aagccgtcca gtgccgaaag cgccggacct tttttggcgg cgaaggcttc agcgcggccc
    41641 acaacgatac agttagtgac aatcagcgga ataaaaatcc ctaatgattg atacaggcca
    41701 aaggcgtagg cgttgatcag catctgtaca gcgctgacca ccgaggcgat gatcatcacg
    41761 taaatgggaa tgcggatctc ggcgggcgtc cagtgacgca gcgtcgaaat ggtcaggttg
    41821 gttagcgtca gtaccagcgt agtcgcaagt cctaaaccca gagcgttagt ggcagtggac
    41881 gtgaccgcca acagcggaca aaggccgagc aactggacca gcgcagagtt gtttttccac
    41941 aacccctgaa caataacgtc tttaatttcg ctcacgtttt attctccaca ggcaggaagt
    42001 tgagaaagtt gtgccggtaa cgtctgagcg tacaatccgg cgcgttttac cgcattaatc
    42061 accgcgcggg gagtaatcgt cgcgccggtg aactggtcga aatcaccacc atctttcttc
    42121 accgcccagt tcgcatcatc cgcaccactg atttttttac ccgcaaaatg ggtgatccag
    42181 tcagaaaggc gcagttcgat tttatcgcca agccctggcg tttcgtggtg ctcagtcacg
    42241 cgcgtgccaa gtaccgtgcc gttaaaatcg gctcctacca gcagctgaat cgcacctgaa
    42301 tagccatctg gcgcggttgc ttccagaacg gcagctaccg gtttgtcatc ctgtttggcg
    42361 atgtaaaccc gatgctcacc tttacctaac tctggcgcag tgaccagata gcaactctgt
    42421 gccagcgcat tgttatagcg ctcagctggc agcacctgat caaataacgc cttttgttgc
    42481 agactggcct gttcagcaat tgtcgttttg gtcatctggt tgatggccgc agttaaccct
    42541 gttgaacccg ctgcaaagag cgccaacgta atgccgtgtt ttcggatagt tttcagcatg
    42601 gtttaccctt tgcgatggcc gtagacgcgc ggacgcgtgt agtaatcgat cagaggaacc
    42661 gtgatgttcg ccagcaggac ggcaaaagcc acgccgtcag gatagccgcc gaaactgcgg
    42721 atcaaccaga ctaataagcc cgccagcgcg ccgaaaataa gacgaccacg attggtcgta
    42781 gaagcggtaa ccgggtcagt caaaataaag aatgcgccga gcatggtcgc tccagacagc
    42841 agatgaattt gcggtgctgc cagtgtttct ggtgagaaca accagcccaa cgttgcgcat
    42901 aacgccagcg ttactaagaa gctgagggga atatgccagc gaatcgcttt ctgccacagc
    42961 aaccatacgc cgccagccag ccaggcgaga tttacccatt gccaaccaac gcccgccaga
    43021 ataccgctgt agatcggata ttgcataatc tgttcaaccg aatgacctgc gcggacagag
    43081 gttttgaatg tatccagcgg tgtcgcctga ctaatgccat caatacctaa gcgtagcgtg
    43141 ttcatatcac caccactggc ggtatgaccg ctgaaaataa cctgtatggc gtcgataaaa
    43201 ccagagatgt tgaccgcaat ttcatgcggc ggtaaccagc tggtcatctg caccgggaag
    43261 gagatcagta agaccacata accaatcatt gccggattaa acgggttttg ccccagaccg
    43321 ccatacaact gtttagcgat gatcaccgca aacaccgtac ccagcacgac catccaccat
    43381 ggcgcgaggg ggggaatact taccgccagc aataagcctg tcagcaatgc tgagttatct
    43441 ttcaacgttg cggctaccga ctgcttgcgt agtttgagta cgagagcttc ggctaacaga
    43501 gcactgaccg acgccagcag gatctgaacg agagtacccc aaccaaaaaa acacagttgc
    43561 gctgcgattc ctggcacggc tgcgagcaac accaacagca taatgcgcga tgtctggcgc
    43621 tggttatggg tataagggga gctagctatt ctgaatacca tttagtcctc gtttacaacc
    43681 ttttgctggg cggctttttt ggcctgaacg cgggcaatag ccgcggcaac tgccgctttg
    43741 cgcggatcaa cctgttcttc tggtaccgca ttagcctgtt gctgttccag cttgcgtgct
    43801 ttggcgcgtg caatagccgc ttcgacggca gctttgcgcg gatcaacctg ttcttctggc
    43861 tccgcattag cctgttgctg ttccagtttg cgcgctttgg cacgggcaat agcggcttcg
    43921 acggcagctt tgcgcggatc gacctgttgt tctgg
//
LOCUS       DAZKHK010000026        42974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKHK010000026 DAZKHK010000000
VERSION     DAZKHK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.026"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(107..1540)
                     /locus_tag="ACHOS5_001466"
     CDS             complement(107..1540)
                     /locus_tag="ACHOS5_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9776296.1"
                     /translation="MKLFRFSKVKIAELPTPEHGQVEYGDTEVNGLRVRVGSSGVKSF
                     CISRKRNGKFIRATLGRFPDLSVGNARAKAMELLGEVATTGKNPNIAKRTNNKAMITL
                     NEALEAYIKNRGHRLKPATEKQYRSILQNYSGDWMAHTLASITRERVEQRHKAVTDGA
                     VWFGADKTTLRAGVGTGSKAQADLWARALRAVCRFAHDHYRDDAGNTLLPDPPTLVLS
                     TKRQWHGTVRKTERIRLHDFSRWFAAVAAVRDQGEQERDDIAVTVCDAVEMALFTGLR
                     KSEIFGLTWDRVNMGGRFFWIDTTKNGDPLELPITDTLREMFRRRLKIKNADDVLVFP
                     GVKGVIQETRHIIDRISAATVSESNDEMLSPVPFKWHDARRTFGTVAELVGVGNYILK
                     RLMNHRTMRSADVTQGYLHFGADELLEPASRIERAILEHAGLVESKKSIDAQLMAVLN
                     NLSEEEKRKLIFSLSTDECNQLWQKEK"
     gene            complement(1820..2584)
                     /gene="nfeF"
                     /locus_tag="ACHOS5_001467"
                     /gene_synonym="yqjH"
     CDS             complement(1820..2584)
                     /gene="nfeF"
                     /locus_tag="ACHOS5_001467"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP9776297.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPGTHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            2872..3495
                     /gene="nfeR"
                     /locus_tag="ACHOS5_001468"
                     /gene_synonym="yqjI"
     CDS             2872..3495
                     /gene="nfeR"
                     /locus_tag="ACHOS5_001468"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP9776298.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENRAQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(3602..5122)
                     /gene="aer"
                     /locus_tag="ACHOS5_001469"
     CDS             complement(3602..5122)
                     /gene="aer"
                     /locus_tag="ACHOS5_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP9776299.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLAIACFEWQIVRPIENVACQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            5540..6919
                     /gene="ygjG"
                     /locus_tag="ACHOS5_001470"
     CDS             5540..6919
                     /gene="ygjG"
                     /locus_tag="ACHOS5_001470"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP9776300.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(6961..7293)
                     /gene="ygjH"
                     /locus_tag="ACHOS5_001471"
     CDS             complement(6961..7293)
                     /gene="ygjH"
                     /locus_tag="ACHOS5_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP9776301.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            7512..8495
                     /gene="ebgR"
                     /locus_tag="ACHOS5_001472"
     CDS             7512..8495
                     /gene="ebgR"
                     /locus_tag="ACHOS5_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP9776302.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYIDQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            8679..11771
                     /gene="ebgA"
                     /locus_tag="ACHOS5_001473"
     CDS             8679..11771
                     /gene="ebgA"
                     /locus_tag="ACHOS5_001473"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP9776303.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGEHVVHSSAIDHLAIEKLTSARF
                     AFTVEQPQQWSAESPYLYHLVMMLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVIEQ
                     SDDEVLLVSRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            11768..12217
                     /gene="ebgC"
                     /locus_tag="ACHOS5_001474"
     CDS             11768..12217
                     /gene="ebgC"
                     /locus_tag="ACHOS5_001474"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP9776304.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            12280..13713
                     /gene="ygjI"
                     /locus_tag="ACHOS5_001475"
     CDS             12280..13713
                     /gene="ygjI"
                     /locus_tag="ACHOS5_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9776305.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            13847..14917
                     /gene="ygjJ"
                     /locus_tag="ACHOS5_001476"
     CDS             13847..14917
                     /gene="ygjJ"
                     /locus_tag="ACHOS5_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP9776306.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAADEARPAEHDDSKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQLFLMQYFYW"
     gene            14934..17285
                     /gene="ygjK"
                     /locus_tag="ACHOS5_001477"
     CDS             14934..17285
                     /gene="ygjK"
                     /locus_tag="ACHOS5_001477"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9776307.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSTERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTDNGEMLFTVKKGNKEETQSGLNNYACVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            17511..19529
                     /gene="fadH"
                     /locus_tag="ACHOS5_001478"
     CDS             17511..19529
                     /gene="fadH"
                     /locus_tag="ACHOS5_001478"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP9776308.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSPLQAPINRFIPHELTHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTITTPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDRIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSKIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQILAVDNVVIC
                     AGQEPNRALAQPPIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(19574..19990)
                     /gene="higA"
                     /locus_tag="ACHOS5_001479"
     CDS             complement(19574..19990)
                     /gene="higA"
                     /locus_tag="ACHOS5_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP9776309.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(19987..20070)
                     /locus_tag="ACHOS5_001480"
                     /pseudo
     CDS             complement(19987..20070)
                     /locus_tag="ACHOS5_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            complement(20261..21397)
                     /gene="rlmG"
                     /locus_tag="ACHOS5_001481"
     CDS             complement(20261..21397)
                     /gene="rlmG"
                     /locus_tag="ACHOS5_001481"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP9776310.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLEKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            21482..21985
                     /gene="ygjP"
                     /locus_tag="ACHOS5_001482"
     CDS             21482..21985
                     /gene="ygjP"
                     /locus_tag="ACHOS5_001482"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9776311.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            22062..22754
                     /gene="ygjQ"
                     /locus_tag="ACHOS5_001483"
     CDS             22062..22754
                     /gene="ygjQ"
                     /locus_tag="ACHOS5_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP9776312.1"
                     /translation="MLRAFARLLLRICFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKAIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            22833..23819
                     /gene="ygjR"
                     /locus_tag="ACHOS5_001484"
     CDS             22833..23819
                     /gene="ygjR"
                     /locus_tag="ACHOS5_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9776313.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            24103..25068
                     /gene="alx"
                     /locus_tag="ACHOS5_001485"
     CDS             24103..25068
                     /gene="alx"
                     /locus_tag="ACHOS5_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP9776314.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVE"
     gene            25392..26636
                     /gene="sstT"
                     /locus_tag="ACHOS5_001486"
     CDS             25392..26636
                     /gene="sstT"
                     /locus_tag="ACHOS5_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9776315.1"
                     /translation="MTTQHSPGLFRRLAHGSLVKQILAGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIHFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(26640..27191)
                     /gene="ygjV"
                     /locus_tag="ACHOS5_001487"
     CDS             complement(26640..27191)
                     /gene="ygjV"
                     /locus_tag="ACHOS5_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP9776316.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(27274..28761)
                     /gene="uxaA"
                     /locus_tag="ACHOS5_001488"
     CDS             complement(27274..28761)
                     /gene="uxaA"
                     /locus_tag="ACHOS5_001488"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP9776317.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGTSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(28776..30188)
                     /gene="uxaC"
                     /locus_tag="ACHOS5_001489"
     CDS             complement(28776..30188)
                     /gene="uxaC"
                     /locus_tag="ACHOS5_001489"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9776318.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            30671..31969
                     /gene="exuT"
                     /locus_tag="ACHOS5_001490"
     CDS             30671..31969
                     /gene="exuT"
                     /locus_tag="ACHOS5_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP9776319.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            32099..32875
                     /gene="exuR"
                     /locus_tag="ACHOS5_001491"
     CDS             32099..32875
                     /gene="exuR"
                     /locus_tag="ACHOS5_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP9776320.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            33115..33198
                     /locus_tag="ACHOS5_001492"
     CDS             33115..33198
                     /locus_tag="ACHOS5_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776321.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            33220..33882
                     /gene="yqjA"
                     /locus_tag="ACHOS5_001493"
     CDS             33220..33882
                     /gene="yqjA"
                     /locus_tag="ACHOS5_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP9776322.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            33886..34269
                     /gene="mzrA"
                     /locus_tag="ACHOS5_001494"
     CDS             33886..34269
                     /gene="mzrA"
                     /locus_tag="ACHOS5_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP9776323.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            34416..34784
                     /gene="yqjC"
                     /locus_tag="ACHOS5_001495"
     CDS             34416..34784
                     /gene="yqjC"
                     /locus_tag="ACHOS5_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP9776324.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            34822..35127
                     /gene="yqjD"
                     /locus_tag="ACHOS5_001496"
     CDS             34822..35127
                     /gene="yqjD"
                     /locus_tag="ACHOS5_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP9776325.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRNKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            35130..35534
                     /gene="yqjE"
                     /locus_tag="ACHOS5_001497"
     CDS             35130..35534
                     /gene="yqjE"
                     /locus_tag="ACHOS5_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9776326.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            35524..35823
                     /gene="yqjK"
                     /locus_tag="ACHOS5_001498"
     CDS             35524..35823
                     /gene="yqjK"
                     /locus_tag="ACHOS5_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP9776327.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLNQQQLRG"
     gene            36010..36402
                     /gene="yqjF"
                     /locus_tag="ACHOS5_001499"
     CDS             36010..36402
                     /gene="yqjF"
                     /locus_tag="ACHOS5_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9776328.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            36472..37458
                     /gene="yqjG"
                     /locus_tag="ACHOS5_001500"
     CDS             36472..37458
                     /gene="yqjG"
                     /locus_tag="ACHOS5_001500"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP9776329.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMQENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            37741..38109
                     /locus_tag="ACHOS5_001501"
     CDS             37741..38109
                     /locus_tag="ACHOS5_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9776330.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(38143..39039)
                     /gene="yhaJ"
                     /locus_tag="ACHOS5_001502"
     CDS             complement(38143..39039)
                     /gene="yhaJ"
                     /locus_tag="ACHOS5_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9776331.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            39144..39845
                     /gene="yhaK"
                     /locus_tag="ACHOS5_001503"
     CDS             39144..39845
                     /gene="yhaK"
                     /locus_tag="ACHOS5_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP9776332.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            39868..40032
                     /gene="yhaL"
                     /locus_tag="ACHOS5_001504"
     CDS             39868..40032
                     /gene="yhaL"
                     /locus_tag="ACHOS5_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP9776333.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(40166..41476)
                     /gene="cyuA"
                     /locus_tag="ACHOS5_001505"
     CDS             complement(40166..41476)
                     /gene="cyuA"
                     /locus_tag="ACHOS5_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP9776334.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(41504..42835)
                     /gene="cyuP"
                     /locus_tag="ACHOS5_001506"
     CDS             complement(41504..42835)
                     /gene="cyuP"
                     /locus_tag="ACHOS5_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9776335.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
BASE COUNT        10282 a        11330 c        10971 g        10391 t
ORIGIN      
        1 ttatttttat cgtcatagca tcgtttaatt atacgtacaa cagaggcttt atcaccgtaa
       61 gtaaataact cacgtataaa aaggtgaatg atataatcag gggttgttat ttttctttct
      121 gccataactg gttacactca tctgtagata aggaaaaaat taatttcctt ttttcttctt
      181 cgcttaggtt gtttaagacc gccatcaatt gcgcatcaat agatttttta ctctccacca
      241 gcccggcatg ttccagaatt gcccgttcta tccgtgacgc tggctccagc agttcatcag
      301 caccaaaatg taaatacccc tgcgttacgt cagcacttcg catagtacgg tgattcatca
      361 ggcgcttgag gatgtaattg cccacgccca ccagttcggc aacagttccg aacgtccggc
      421 gcgcatcgtg ccatttgaat ggtacagggg agagcatttc atcgttcgac tctgaaacgg
      481 tagccgcgct tatccggtct atgatgtgcc gtgtctcctg aataacgccc ttcacaccag
      541 gaaacaccag aacatcatca gcgtttttta ttttcagccg gcggcggaac atctcccgca
      601 acgtatcagt gatcggcaat tcgagcggat ccccgttttt cgtggtgtct atccagaaaa
      661 aacgaccgcc catattcacg cgatcccacg taaggccaaa aatttctgat tttctcagtc
      721 cggtaaaaag cgccatttcc acggcatcac ataccgtcac cgctatatca tccctttcct
      781 gttcgccctg gtcgcgtaca gctgctacag cagcaaacca gcgcgaaaaa tcatgcaggc
      841 ggatacgttc cgtttttctt accgttccgt gccactggcg cttagtgctc agtaccagcg
      901 tcggcggatc cggtaaaaga gtattacccg catcatcacg ataatgatca tgcgcaaacc
      961 gacacacggc acgcagcgca cgcgcccata aatcggcctg tgccttgctt cctgtaccaa
     1021 ccccagcccg taacgtggtt ttatcagcac cgaaccatac agccccgtct gtgacagcct
     1081 tatgccgctg ttccacacgt tcgcgggtaa tactggcgag ggtatgggcc atccaatcac
     1141 cggaataatt ctgtaatatg ctgcggtact gtttttctgt ggcgggtttc aggcggtggc
     1201 cacgattttt tatgtaggct tcaagcgcct cattcagcgt tatcatggcc ttgttgttcg
     1261 tgcgcttcgc tatgttcggg tttttccccg ttgtggcgac ctctccaagg agttccatag
     1321 ctttagccct ggcattgccc acactcagat caggaaaacg ccccagcgtg gcacgaataa
     1381 attttccgtt acgcttacgg gaaatgcaga aacttttcac accagacgaa cccacacgga
     1441 cacgcagccc gttaacctcc gtatcaccat attcgacctg tccatgttcc ggcgttggca
     1501 gttctgcaat ttttacttta gaaaacctga atagtttcac tcataccccc taccaagcga
     1561 tctggcatcc tggaaaatcg cttgttctgc tccaggatac ctataggata ccgtaaagaa
     1621 gtatttagag gtatatacag gtatttatac agtagtttta aaaaatacta aggtattgaa
     1681 aagtatagtt aattatctgt aagtaaagag ttatcaacca actcataatc gcttggtcgc
     1741 tggttcaagt ccagcagggg ccaccagaca tagcaaaggc tgacgagaaa tcgtcagcct
     1801 ttttcttttt atatatcagt tactttgcgt gccagtaagc cgccgcgcgt acccgctgtg
     1861 ggtcatactg ttccgcttca aagcgacggc ttaaattctt aacgactttg ccttcgccgg
     1921 ttatccagat gaagtaatca tcggcaggga tttgcatctg cgccagacgc gcatctaccg
     1981 cctgctcatc atgtgccagc cattcgatat taaaaccatc aaggtgcgcg agataatcct
     2041 gacaggcgtt atcccgcacg ctaaccagcg cactaacttg cggtttaacg gcaagtttgc
     2101 tcaacgtttc caggcggcgg cgcagtgcgg gcattccgga ttcatcgcag acatacagct
     2161 gatacgcgta atcctctggc accaccagcg aaccgcgcgg acctgccacc gtaagtttat
     2221 cgcccggttt cgcctgcatc gcccagccgc tggcgacccc accgtcgtga ataaagaaat
     2281 cgatcgccag ttcatggcgt agttcgtcat acagcggcgt atagtcacgc gacggtgggc
     2341 gtggtccttc cggccagacg atgccctctt ccgttaccgt tggcggcaca aagtgagtgc
     2401 ctggttgagg aaagaagagc ttgctgtgat cgtcaaagcc gcgcgatgta aaaccgtcca
     2461 gcgcctcgcc gccaaggaca atgcgctgaa aaccggcgct gatacgctca acgcgtagca
     2521 cagtcagttc acggaagcgc agatcattgc gaacgcgctg cgggtagcgg ggggagttat
     2581 tcattgttat cgccttcatg atggtaatca gatatatcta aataaattcc acaaatgata
     2641 atgattgtta atcatgataa atgcaagtga tttgtagcac tgatatatct gtagtctgat
     2701 aagacgcgca acctcttatc aagcatcatt gctcaatgcc ggatgcggcg tgaacgcctt
     2761 atccggccta catgttgagt aaacctgcgt ttaaaaatca tttttgtact tgcagtaatg
     2821 cttatatcag atataaatta gatatatcta attaagcaaa aggaggctga tatgagccat
     2881 catcacgaag ggtgctgtaa acatgaaggc cagccacgcc atgagggctg ctgcaaaggt
     2941 gagaagtcag aacacgagca ctgcggacac ggtcaccagc atgaacacgg tcaatgctgc
     3001 ggtggtcgcc acggtcgcgg cggcggtcgt cgacaacgtt tctttggtca cggtgaattg
     3061 cgtctggtga ttctggatat tctctcgcgc gatgacagcc acggttacga gttgattaaa
     3121 gcgattgaaa atctgaccca ggggaattac accccaagcc cgggcgtcat ctacccgacg
     3181 ctggattttc tgcaggagca gtcgctgatt accatccgcg aagaggaagg tggtaagaag
     3241 cagattgcgc tgaccgaaca aggcgcgcag tggctggaag aaaaccgcgc acaggtagag
     3301 atgattgaag aacgcatcaa agcgcgttgc gttggcgctg cgctgcgcca gaacccgcaa
     3361 atgaagcggg cactggataa ttttaaagcg gtgctggatt tacgcgtcaa ccagagcgat
     3421 atcactgatg cacaaataaa aaagatcatt gcggtgatcg accgcgccgc ttttgatatt
     3481 acccaactgg attaatcacc gattgccgga tgcggcgtac cctacacacc ccgtaggcct
     3541 gataagatgc gccagcatcg catcaggcat tgtgctccaa ccgctggatc cggcataccg
     3601 attaatgcag caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc accatcgccg
     3661 acacctgcgc actctcttcc accaactcgg cgtttttctg ggtgatcagg ttaagctcat
     3721 ccactgcacg ggtcaggctg gaaagcccat cggcctgttc cagcgttgaa tggctaatct
     3781 gagctatcaa ctgggtgacg ttttttacct gcgccacaat atcttccatc gtccgtccgg
     3841 cggcgtgtac ctgctgcgaa ccagattgca ccttatcagc actggcatca atcagcttgc
     3901 gaatatcgtt ggcagcattg gcgctgcggc tggctaaatg acgcacttcc cccgccacca
     3961 ctgcaaaacc tttgccctgt tcgccggcac gcgccgcttc caccgccgca ttcagggcca
     4021 gaatattggt ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca atgcgctggg
     4081 tactgtcggc aatatcgtcc attgtcttga tcaccgtagt catcgcctcc ccgccctgca
     4141 ctgccgcatt actggcggtg atagaaagtt tatcggcagc cgacgccgtg gcagagttct
     4201 gtttcaccga cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg ttatcaactg
     4261 tctgctgggt atgttcgttc agttcatcgg tgcctttcgc cagcgtctca ctgccgtttc
     4321 tgacactgga cacctggctt gagacatcat taattagcca acggcacatc aggccaagct
     4381 gccctaccgc acgtaatgtc agccccagct catcgctgcg cttcagatgc tcaacactat
     4441 tacgctctcc ggtcgccacc ttcagtgcct gacaggcgac attttctatc gggcggacaa
     4501 tctgccattc aaaacaagcg attgccaaca ataccactaa tgcacagagg atatacgtca
     4561 ccaccggggc agcaacaaac cagagcatgg ccgccagcaa gataaacatc agggtcatca
     4621 ctccacgcgc ccgccagcga agcggtaatg aaggcagttt tcccaaccaa cctttacgca
     4681 ccaccaggcc tttatgaata cgcttactgg tccgtccggc gttcaacgct ttgtacagcg
     4741 gctccaccgc cgcgatctct tcatccgtcg cccgggtacg aatcgacata tagccactga
     4801 tttttccctc gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg tcgccatttt
     4861 tgcggcgatt tttcacgatg ccgctccagg gctccccttt tttcagggtg aaccacatat
     4921 ccgcaaacgc cgcttttggc atatccgggt gacgcaccat gttgtgcggt tgcccctgta
     4981 actcctgcaa ggtatagccg ctcacctgca caaaagtgtc attagcatga gtgatatagc
     5041 tttgcagatc ggtagtggac atcagagtgg tgtcgtccgc cagcggggta ttttgctggg
     5101 tgacatacgg atgagaagac atgttcgcgt cctgtgcagg ttatatggtt gttaacttct
     5161 tgtcagagtt tatgtcggcc ccgctgcggt tatctttaac caattaattt gatttagatc
     5221 gcaatttgcg actaaagcac aaatctaatt ccttgattta aaatactttc actctgttac
     5281 tatacgaaaa cgttaattac cttgcccaaa aatcaggcaa ttattgccct gaaagcgtgc
     5341 atttgcgcag caatcatcaa atccataccc gacaaaaacc gtgcaaaata acagcaaatg
     5401 ttagcaaata gcattaaata ttgcgcaaat gataaccaca tttgtgttta tcccgatttt
     5461 cgcgatcgca gccggagtgg cgcaatccct gcaatactta aatcggtatc atgtgatacg
     5521 cgagcctccg gagcatattt tgaacaggtt accttcgagc gcatcagctt tagcgtgcag
     5581 cgcccacgcc ctgaatctca ttgaaaagcg aacgttggat catgaggaga tgaaagcact
     5641 taaccgggag gtgattgaat acttcaaaga gcatgtcaat ccggggtttt tagagtatcg
     5701 caaatctgtt accgccggcg gggattacgg agccgtagag tggcaagcgg gaggtttaaa
     5761 tacgcttgtc gacacccagg gacaggagtt tatcgactgc ctgggaggtt ttggaatttt
     5821 caacgtgggg caccgtaatc cagttgtggt ttccgccgta cagaatcaac ttgcgaaaca
     5881 accgcttcac agccaggagc tgcttgatcc gttacgggcg atgctggcga aaacccttgc
     5941 ggcgctaacg cccggtaaac tgaaatacag cttcttctgt aatagcggca ccgaatccgt
     6001 tgaagcggcg ctgaagctgg cgaaggctta tcagtcaccg cgcggcaagt ttacttttat
     6061 tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg ctgtcggcca cggcgaaatc
     6121 gaccttccgc aaaccgttta tgccactgct gccagggttc cgtcatgtac cgtttggcaa
     6181 tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa accggtgatg atgttgctgc
     6241 ggtgatcctc gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc caggctatct
     6301 caccgccgtg cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg atgaagtaca
     6361 aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag catgagaacg tacagccgga
     6421 tatcctttgt ctggcaaaag cgctcggtgg cggcgtgatg ccgattggcg caaccatcgc
     6481 cactgaagag gtgttctcag tcctgttcga caatccgttc ctgcatacca ccacctttgg
     6541 cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc tggagcagaa
     6601 cttaccggct caggctgaac aaaaaggcga tatgttgctg gacggtttcc gtcaactggc
     6661 gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa gggatgttga tggcgattga
     6721 gtttgttgat aacgaaatcg gctataactt tgccagcgag atgttccgcc agcgcgtcct
     6781 ggtggccgga acactcaaca acgctaaaac gatccgcatt gaaccgccac tgacactgac
     6841 cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg ccatgcgagt
     6901 cagtgtcgaa gaagcgtaat atcaaatcgg atggcgattc gacgtcgcca tccgatcttt
     6961 ttatacgata cgtacgcccg caggcatcat ccgctccggc gttaacagca cgctttcact
     7021 gccgtcatcg gtttccgcac acaacagcat gcattctgac gtttcaccgc gcattttcgc
     7081 tttttgcaga ttgcataaga ccaccactgt tttccccatc agctcttctt cgctgtagta
     7141 cggcaccagg ctggtcacgg tttgcagcgt tttttcaccc acatcaacct gtacgatgta
     7201 cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc tttccgacac gcatttccag
     7261 acgtgcaaaa tcagcataag ccacggtttc cattgtttcc tcccttcgag taaaatttta
     7321 ctaaactata gcaaaagttt ttctcgatcc tgtaggctaa aaatggagaa tgcaggcgtg
     7381 atcacattcg tgagccgctg tgttaccgtt acagcgtcaa agaaacgcgc tttatttact
     7441 gaaaacaggt gacccgataa gcacttcctc tacaatggag gcgcacatca gggaaagtaa
     7501 aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga agctggcgta tccctggcga
     7561 cagtatccag ggtcttgaat gacgatccga cattgaatgt gaaagaagag acgaaacatc
     7621 gcattctcga gatcgccgaa aagctggagt acaagaccag tagcgcccgt aaactccaga
     7681 caggcgcagt caaccaacac catattctgg ctatctacag ctaccaacag gagctggaga
     7741 tcaacgatcc ttactatctg gcgatccgcc acggcattga aactcagtgc gaaaagctgg
     7801 cgatcgaact gaccaactgt tatgaacaca acggcttacc agacattaaa aacgtcaccg
     7861 gtattttaat tgtcggcaaa cccacgcccg ccctgcgcgc cgctgccagc gcattgaccg
     7921 acaatatctg ttttatcgac tttcacgaac ccggcagcgg ttacgatgcg gtggatatcg
     7981 atctggcacg catcagtaaa gaaatcatcg atttctatat cgatcagggt gttaatcgta
     8041 ttggttttat tggcggtgaa gacttacccg gcaaggcgga tattcgtgag gtcgcctttg
     8101 cggaatatgg tcgtctgaaa caagtggtac gcgaagagga tatctggcgc ggcggttttt
     8161 ccagttcgtc gggctatgaa ctggcaaaac agatgctggc gcgggaagac tatccgaagg
     8221 cactgtttgt tgcttccgat tccattgcta tcggcgtact gcgggcaatt catgaacgtg
     8281 gcctgaacat cccacaggat atttcgctta tcagcgttaa cgatatcccc accgcgcgat
     8341 ttacctttcc gccgctctcc accgtgcgca tccattccga gatgatggga agtcagggcg
     8401 ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct gccgctgttg gtcttcgttc
     8461 ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc cttgcacgct gtccagcaat
     8521 cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc agttaaagta aatgcattta
     8581 ctcgctactt tttagtaaaa tttttactaa actccccagc aattacacaa actaccatca
     8641 ccatgaatgg ttccgatttc tctctaccgg gaggccctat gaatcgctgg gaaaacattc
     8701 agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt tttttcatat gattctgttg
     8761 cgcaggctcg tacttttgcc cgtgaaacca gcagcctgtt tctgccctta agcggtcagt
     8821 ggaatttcca cttttttgac catccgctgc aagtgccaga agccttcacc tctgagttaa
     8881 tggctgactg ggggcatatt accgtccccg ccatgtggca aatggaaggt cacggcaaac
     8941 tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt gccgtttgtc cccagcgata
     9001 acccaaccgg tgcctatcaa cgtattttca ccctcagcga cggctggcag ggtaaacaga
     9061 cgctgattaa atttgacggc gtcgaaacct attttgaagt ctacgttaac ggtcagtatg
     9121 tggggttcag caagggcagt cgcctgaccg cagagtttga catcagcgcg atggttaaaa
     9181 ccggcgacaa cctgttgtgt gtgcgtgtga tgcaatgggc ggactctacc tacgtggaag
     9241 accaggatat gtggtggtcg gcggggattt tccgcgatgt ttatctgatc ggaaaacaac
     9301 tcacgcatat taacgatttc accgttcgta ccgactttga cgaagcctat tgcgatgcca
     9361 cactttcctg cgaagtggtg ctggaaaatc tcgccgcctc ccctgtcgtc acgacgctgg
     9421 aatataccct gtttgatggc gaacacgtgg tgcacagcag cgccattgat catctggcaa
     9481 ttgaaaaact gaccagcgcc cgctttgctt ttactgtcga acagccgcag caatggtcag
     9541 cagaatcccc ttatctttac catctggtca tgatgctgaa agacgccgac ggcaacgttc
     9601 tggaagtggt gccacaacgc gttggcttcc gtgatatcaa agtgcgcgac ggtctgttct
     9661 ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg ccacgacaac gatcatcgca
     9721 aaggccgcgc cgttgggatg gatcgcgtcg agaaagatct ccagttgatg aagcagcaca
     9781 acatcaactc cgtgcgtacc gctcactacc cgaacgatcc acgtttttac gaactgtgcg
     9841 atatctacgg cctgtttgtg atggcggaaa ccgacgtcga atcgcacggc tttgctaacg
     9901 tcggcgatat cagccgcatt accgacgatc cgcagtggga aaaagtttac gtcgagcgca
     9961 ttgttcgcca tattcacgcg cagaaaaacc atccgtcgat catcatctgg tcgctgggca
    10021 atgaatccgg ctatggctgt aacatccgcg cgatgtacca tgcggcgaag gcgctggatg
    10081 acacgcgact ggtgcattac gaagaagatc gcgatgctga agtggtcgat attatttcca
    10141 ccatgtacac ccgcgtgcct ctgatgaatg agtttggtga atacccgcat ccgaagccgc
    10201 gcatcatctg tgaatatgct catgcgatgg ggaacggacc gggcgggctg acggagtacc
    10261 agaacgtctt ctataagcac gattgcattc agggacatta tgtctgggaa tggtgcgacc
    10321 acgggatcca ggcgcaggat gacaacggca acgtctggta caaattcggc ggcgactacg
    10381 gcgactatcc caacaactat aacttctgtc ttgatggttt gatctattcc gatcagacgc
    10441 cgggaccagg cctgaaagag tacaaacagg ttattgcgcc ggtaaaaatc cacgcgctgg
    10501 atctgactcg cggcgagttg aaagtcgaaa ataaactatg gtttaccacg cttgatgact
    10561 acaccctgca cgcagaggtg cgcgccgaag gtgaaacgct cgcgacgcaa cagattaaac
    10621 tgcgcgacgt tgcgccgaac agcgaagccc ccttacagat cacgctgccg cagctggacg
    10681 cccgcgaagc gttcctcaac attacggtga ccaaagattc ccgcacccgc tacagcgaag
    10741 ccgggcattc tatcgccact tatcagttcc cgctgaagga aaacaccgcg cagcctgtac
    10801 ctttcgcacc gaataacgcc cgtccgctga cgctggaaga cgatcgtttg agctgtactg
    10861 ttcgcggcta caactttgcg atcaccttct caaaaacgag tggtaaaccg acatcctggc
    10921 aagtgaatgg cgagtcgctg ctgacccgcg agccaaagat caacttcttc aagccgatga
    10981 tcgacaacca taagcaggag tacgaagggc tgtggcagcc aaatcatttg cagatcatgc
    11041 aggagcatct gcgcgatttt gtcattgagc aaagtgatga tgaagtgttg ctagtcagcc
    11101 gcacggttat tgccccaccg gtgtttgact tcgggatgcg ctgcacctac atctggcgca
    11161 tcgctgccga tggccaggtt aatgtggcgc tttccggcga acgttatggc gactatccgc
    11221 acatcattcc gtgcatcggt ttcaccatgg gaattaacgg cgaatacgac caggtggcgt
    11281 attacggtcg tggaccgggc gaaaactacg ccgacagcca gcaggctaac atcattgata
    11341 tctggcgcag caccgtcgat gccatgttcg agaactatcc cttcccgcag aacaacggta
    11401 accgtcagca tgtccgctgg acggcactga ctaaccgcca cggcaacggt ctgctggtgg
    11461 ttccgcagcg accaattaac ttcagcgcct ggcactatac ccaggaaaac atccacgctg
    11521 cacagcactg taacgagctg cagcgcagtg atgacatcac cctgaatctc gaccaccagc
    11581 tgcttggcct cggctccaac tcctggggca gcgaggtgct ggactcctgg cgcgtctggt
    11641 tccgtgactt cagctacggc tttacgttgc tgccggtttc tggcggagaa gctaccgcgc
    11701 aaagcctggc gtcgtatgag ttcggcgcag ggttcttttc cacgaatttg cacagcgaga
    11761 ataagcaatg aggatcatcg ataacttaga acagttccgc cagatttacg cctctggcaa
    11821 gaagtggcaa cgctgcgttg aagcgattga aaatatcgac aacattcagc ctggcgtcgc
    11881 ccactccatc ggtgactcat tgacttaccg cgtggagacg gactccgcga ccgatgcgct
    11941 atttaccggg catcgacgct attttgaagt gcattactac ctgcaagggc agcaaaaaat
    12001 tgaatatgcg ccgaaagaga cattacaggt agtggaatat tatcgtgatg aaactgaccg
    12061 tgaatattta aaaggctgcg gagaaaccgt agaggtccac gaagggcaaa tcgttatttg
    12121 cgatatccat gaagcgtatc ggtttatctg caataacgcg gtcaaaaaag tggttctcaa
    12181 agtcaccatc gaagatggtt atttccataa caaataacaa ctacggcggc aaaaggagtt
    12241 tgccgccacc gctaccctac tcattttcgg agatgtgtta tgtctgatac caaacgtaat
    12301 acaatcggca aattcggctt gctctcactg acttttgccg ccgtttacag ctttaacaac
    12361 gtcatcaata ataatattga gcttggactg gcctcggcac cgatgttttt cctcgcgacg
    12421 attttttatt ttattccctt ctgtctgatc atcgcagaat ttgtttcgtt aaataaaaac
    12481 tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg gcggacgttg ggcatttatt
    12541 actgcctata cctactggtt cgtaaacctg ttctttttca cctcgctgtt accgcgcgtt
    12601 attgcttatg cttcgtatgc cttcctcggc tacgaatata ttatgacgcc ggttgccacc
    12661 accattatca gtatggtgct gttcgccttc tccacctggg tttccaccaa cggggcgaaa
    12721 atgctggggc caattacctc cgtcacttca acgctgatgc tgctgttaac gctctcctac
    12781 attttactgg caggtacggc actggttggc ggcgtacagc ctgccgatcc gatcaccgtt
    12841 gacgcgatga tcccgaactt caactgggcg ttcctcggcg tgaccacctg gatctttatg
    12901 gccgcaggtg gcgcggagtc cgtcgcggtg tacgttaacg acgtcaaagg cggttcgaaa
    12961 tcgttcgtta aagtgatcat cctcgccggg atttttatcg gcgtactgta ttccgtctcc
    13021 tcggtgctga ttaacgtctt cgtcagcagc aaagagttga aatttactgg cggatcggtg
    13081 caggtattcc acggcatggc ggcgtatttt ggtctaccgg aagcgttgat gaatcgcttt
    13141 gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc tgatgtggac ggcaacgccg
    13201 gtgaaaattt tcttctccga aatcccggaa ggcatctttg gtaagaaaac cgtcgaactt
    13261 aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt tcctgatcgt catcccgctg
    13321 atgattatcc cgatgctcgg ttccaatacc gtgcaggatc tgatgaatac cattattaat
    13381 atgaccgccg cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta
    13441 cgcgccaaat tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt
    13501 atcgttgttt caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg
    13561 actggcgcga atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctc
    13621 ggctttgcgt ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat
    13681 attgaagcga cgccagccag caatgttgat taagcagact aataaaaaat gagcaggcat
    13741 tttccctctt cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta
    13801 caacgataaa aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac
    13861 tgcaccatgc agagcattac ttgctctgcc gttttgctac gccttttctg cggccgctga
    13921 tgaagcacgt ccggcagaac atgacgactc aaaaaccccc gcaatgacct cgacatcttc
    13981 tccttcattt cgtttttacg gcgaattagg ggttggtggg tatatggatt tagagggtga
    14041 gaataaacat aaatacagcg acggcaccta tattgaaggt ggcctggaga tgaagtacgg
    14101 ctcctggttc ggcctgattt acggcgaagg ctggaccgtg caggccgacc acgacggtaa
    14161 tgcctgggtg cctgaccata gctggggtgg tttcgaaggt gggattaacc gtttctatgg
    14221 cggttatcgt accaatgatg gcaccgaaat catgctcagt ctgcgtcagg attcctcgct
    14281 ggatgacctg caatggtggg gcgatttcac gcccgatctg ggctacgtca ttcccaatac
    14341 ccgcgacatt atgactgcgc tgaaggtaca gaacttaagc ggcaactttc gttatagcgt
    14401 taccgccaca cctgccggac atcatgacga aagcaaagcc tggctacatt ttggcaagta
    14461 cgatcgctac gacgacaaat acacctatcc ggcaatgatg aacggttaca tccagtatga
    14521 ccttgccgaa ggcatcacct ggatgaacgg tctggaaatc accgacggca caggacaact
    14581 ctatctcacg ggcctgctta cccctaactt tgccgctcgc gcctggcacc atacaggacg
    14641 cgccgacggg ctggacgtac cgggaagtga aagtgggatg atggtgagcg ccatgtatga
    14701 agcgttaaag ggcgtttatc tctccaccgc ttacacctac gccaaacatc gccctgacca
    14761 cgctgacgat gaaaccacct ctttcatgca gtttggtatc tggtacgaat acggcggcgg
    14821 acgtttcgca acggcttttg atagccgctt ctacatgaaa aatgcctcta acgatcccag
    14881 cgaccaactc ttcctgatgc aatatttcta ctggtaataa ggactgtaat ttcatgaaaa
    14941 tcaaaactat tttaacgcca gtaacctgcg ctctgctgat aagtttttcc gcccatgccg
    15001 ctaacgccga caattataaa aacgtgatta accgtactgg cgcgccgcag tacatgaagg
    15061 attacgatta cgacgatcac cagcgtttta atccgttttt cgatctcgga gcctggcatg
    15121 gtcatctgtt gccagacggc ccgaacacca tgggcggctt tccgggcgtt gcgctgctga
    15181 cggaagagta catcaacttt atggccagca atttcgaccg cctgaccgtc tggcaggacg
    15241 gcaagaaagt cgacttcacg ctggaggcat acagtattcc cggcgcgctg gtgcaaaagt
    15301 tgatatcgaa agatgtacag gtcgaaatga ttctgcgctt cgccacgccg cgcacatcac
    15361 tactggaaac caaaatcacc agcgataaac cgctggatct ggtgtgggat ggcgaactgc
    15421 tggaaaaact ggaagcgaaa gaagggaaac cgctttccga taaaaccatt gctggcgagt
    15481 accctgacta tcagcgcaaa atcagcgcca cccgtgatgg cctgaaagtc acctttggca
    15541 aagtgcgcgc cacctgggat ctgctgacct ccggcgaatc ggaatatcag gtgcataaat
    15601 ccctgccggt gcagactgaa atcaacggta atcgctttac cagtaaggcg catatcaacg
    15661 gttcgacgac gctctatacc acctactccc atctgctgac cgctcaggaa gttagcaaag
    15721 agcaaatgca gatccgcgat attctggcgc gtccggcgtt ttatctcacc gcctcgcagc
    15781 aacgctggga agagtatctg aagaaagggt taaccaatcc ggatgcgacg ccggaacaga
    15841 cgcgcgtcgc ggtgaaagcc atcgaaacgc tcaacggtaa ctggcgatcg ccgggcggtg
    15901 cggtgaaata taacaccgtc acgccgtcgg tgaccgggcg ctggttctcc ggcaatcaga
    15961 cctggccgtg ggatacctgg aagcaggcgt ttgcgatggc gcatttcaat ccggatatcg
    16021 ccaaagagaa tatccgcgcg gtcttctcct ggcagatcca gccaggcgac agcgtgcgtc
    16081 cgcaggatgt gggctttgtc cccgacctga tagcgtggaa ccttagcacc gagcgtggcg
    16141 gcgatggcgg caactggaac gaacgtaaca ccaaacccag ccttgccgcc tggtcggtga
    16201 tggaagtgta caacgtcacc caggataaaa cctggctggc agagatgtac ccgaaactgg
    16261 tggcctatca cgactggtgg ctacgtaacc gcgatcacaa cggcaacggc gtaccggaat
    16321 atggcgcaac ccgcgataaa gcccacaata ctgataatgg cgagatgctg tttacagtga
    16381 aaaaaggcaa taaagaagag acgcagtctg ggctgaacaa ctacgcatgc gtggtggaga
    16441 aaggccagta cgacagtctg gaaattccgg cacaggttgc ggcgtcgtgg gaatcggggc
    16501 gtgatgatgc cgccgtcttt gggtttatcg acaaagaaca gttggataaa tatgtcgcta
    16561 acggcggcaa acgtagcgac tggacggtga aattcgccga gaaccgcagt caggacggaa
    16621 cgttgctggg ctactcgcta ttgcaggagt cggtggatca ggccagctat atgtacagcg
    16681 ataaccatta tctggcggag atggcgacga ttctcggtaa gccggaagag gccaaacgct
    16741 atcgtcagtt agcacaacag ctcgcggact acatcaacac ttgtatgttc gacccgacta
    16801 cacagttcta ctatgacgtg cgtattgaag ataaaccgct ggcgaacggc tgcgcgggca
    16861 aaccgattgt cgagcgcggt aaagggccgg aaggctggtc accgctgttt aacggtgcgg
    16921 caacgcaggc caacgccgac gcggtggtga aggtgatgct ggatccgaaa gaattcaaca
    16981 cctttgtccc gctggggacg gcagcgttaa ccaacccagc ctttggcgca gatatctact
    17041 ggcgcgggcg cgtatgggtg gatcagttct ggtttggtct gaaagggatg gagcgttacg
    17101 gttatcgcga tgacgccctg aaactggctg atacgttctt ccggcacgcc aaagggttaa
    17161 ccgccgatgg cccgattcag gagaattaca acccgctcac tggcgcgcag caaggcgcac
    17221 caaatttctc ctggagtgcc gcgcatttgt atatgttgta taacgatttt ttccgtaagc
    17281 agtaatttgc aacggctggc ggatgcggcg tgaacgcctt atccgcccta catgtgcaat
    17341 cccccaggcc ggataagacg cgtcaagcgt cgcatccggc gttaccaccg ggcgtattct
    17401 ttttgaatcc catcacaaac cccgcactcc ccttttccct tttctccggc gacggctaaa
    17461 ttagaactca tccgaccaca taacaattat tttacatact ggacaatttt atgagctacc
    17521 cgtcgctgtt cgccccgctg gatttaggtt ttaccacgtt aaaaaaccgc gtgttgatgg
    17581 gttcaatgca caccgggctg gaggaatacc cggacggcgc tgaacggctg gcagcgtttt
    17641 atgccgaacg cgcccgtcac ggcgtggcgt tgattgtcag cggcggtatc gcaccagatt
    17701 taacaggcgt tggcatggaa ggcggtgcaa tgctcaatga cgccagccag atcccacacc
    17761 atcgcaccat taccgaagcc gtacatcagg aaggcggcaa aatcgccctg caaattttgc
    17821 ataccgggcg ctacagctac cagccgcacc tggttgcacc ctctccgttg caggcaccaa
    17881 ttaaccgttt cattccccat gaactcaccc atgaagagat tctgcaactg atcgacaatt
    17941 tcgcccgctg tgcgcaactg gcgcgagaag caggatacga tggcgtagag gtaatggggt
    18001 ccgaagggta tttgatcaac gagtttctta ccctgcgcac caatcagcgt agtgaccagt
    18061 ggggtggcga ttaccgcaac cggatgcggt ttgccgtaga agtagtgcgt gcggtgcgcg
    18121 aacgtgtcgg caacgacttc attattatct accgtttgtc gatgctcgac ctggtcgaag
    18181 gcggcggtac ttttgccgaa acggtagagc tggcgcaggc cattgaagcg gcgggcgcaa
    18241 cgattatcaa caccggcatt ggctggcatg aagcgcggat tccgaccatc accacgcccg
    18301 tgccgcgcgg cgcatttagc tgggtcacac gcaaactgaa aggccacgtt tcgctgccac
    18361 tggtgaccac caaccggatt aacgatccgc aggttgccga cgatattctc tcgcgcggtg
    18421 atgcggacat ggtatcgatg gcgcgaccgt ttctcgctga tgcggagctg ctatcaaaag
    18481 cgcaatcggg acgagccgat gagatcaaca cttgtattgg ctgcaaccag gcctgtctcg
    18541 atcggatctt cgttggcaaa gtcacctcgt gcctggtgaa tcctcgcgcc tgccatgaaa
    18601 ccaaaatgcc aatccttcct gccgtgcaga aaaaaaatct ggcggtggtc ggtgcgggac
    18661 ctgctgggct ggcgtttgcc attaacgcgg cggcacgtgg gcatcaggta acattgtttg
    18721 atgcgcacag caagatcggt gggcagttta atatcgccaa acagatcccc ggcaaagagg
    18781 agttttacga aacgctgcgc tattaccgcc ggatgatcga agtgacgggt gtgacgctaa
    18841 aactcaatca caccgtgaca gcggatcagt tacaggcgtt cgatgaaacg atcctcgcca
    18901 gtgggatcgt gccgcgcgtt ccgcctatcg acggaatcga tcatccgaag gtattgagtt
    18961 atctcgatgt actgcgcgac aaagcgccgg tgggcaacaa agttgccatc atcggttgtg
    19021 gcggaattgg ttttgatacg gcgatgtatt taagtcagcc gggcgaatcc accagccaga
    19081 atatcgccgg gttctgtaat gagtggggaa tcgacagtag cctgcaacag gccggaggtt
    19141 tgagtccgca aggcatgcag atcccccgta gcccacggca gattgtgatg ctccagcgca
    19201 aagccagcaa accaggacaa gggttaggca aaaccaccgg ctggatccat cgcaccaccc
    19261 tgctctcacg cggcgtgaaa atgatcccag gcgtaagtta ccagaagatc gacgatgacg
    19321 ggctgcatgt agtgatcaac ggcgaaacgc agatattagc agtggacaat gtggtgatct
    19381 gcgcagggca agagccaaac cgcgcattgg cgcaaccgcc gattgatagt gggaaaactg
    19441 tgcatttaat tggcggctgc gatgtggcta tggagctgga cgcacgacgg gcaattgccc
    19501 agggaacacg gctggcgctg gagatttaaa tcgttgtttg aagcgccgga tgcgacgcat
    19561 ccggcacgtc gcgttaatca ataaacaagg cgggagaaat gccgaatcgc gttgccagtt
    19621 ttttagcgtg ttccagcgtt aatttcctct tcccgctcaa aacgcgtgac accatcgatt
    19681 tactgccaat ttccggaaga tcggaaaggg ttaaaccata ttgatccata agggtacgaa
    19741 tcacagctat accgccaggc atggcttgcg ccatggcatt aaattcagca aattcgggcg
    19801 ctgattcttc ccacgcggtt attttggcac acaccagatc cagcaagggg ttttcaggat
    19861 cgttgagcag cagatgatct accagttcca gcgcctgggt gtattgttcc tcgttctgaa
    19921 tacccgccag aaaaggtgcc acagcagtta gcttttctcc tgcttgcaag atgtcggcaa
    19981 tcgcaatcat tttttcccct tagtacgatg aacggcggta aagaaatcgt attctttatg
    20041 cgtcataact tcacgtatgt agcataaacg ttgaacaacg ctaaacaaga gaatgtagag
    20101 gatggtgaca gggatttttt gttttatgga agcgcgattc cagaactggg cagacattgc
    20161 cggatgcgac gcttgccgcg tcttatccga cctgggggta atgtgctgaa cgtatgccag
    20221 ataaggcgtt tacgccttat ctggcaggta gctgccaatc ttaccgacga cgccccagct
    20281 tcaccgcttt cagcaccaca aatttattat tcgtcgcaat agtggtgcag ttgccgaaaa
    20341 ttttcttcag tttgtggaag taatccaggt gacggttggc aacgatatac agctcgccgt
    20401 taattttcag gcagcggcgg gcgtggtgga acatctccca ggcgacgtta tcggtcagcg
    20461 catgttgctg atgaaacggc gggttgcaga gcacagcatt aaaacggaaa ggctccacgc
    20521 cggagagcgc gttgttgatc ataaactcgc agcgatccaa cgcctctggc atgttggttt
    20581 caacgttcaa acggctggaa gcaaccgcca tcggcgattc atcgacaaac accactttcg
    20641 cctgcgggtt tttctcgagc aatgtcaggc caatgacgcc attaccgcaa ccgagatcga
    20701 caatctcacc ttcgagattc tctggcagat gttgcataaa gaagcgcgcg ccaatatcca
    20761 gcccagtgcg ggagaaaaca ttcgcatggt tgtggatagt ccagtcagta ccttccagct
    20821 tccagctaac ggtctgcggc gcatcggcca acggcggttc gttgaaggtg cagttaatca
    20881 ggcgcgcttt cttccatgcc agcgtggtgg tggtcggacc gagcactttt tcgaacaatt
    20941 ccagcgtgga agtgtgaatg tcacgggctt tggcaccggc aataatgcgc gtatctggcg
    21001 tgaccacttt gcgcagcgcg cgcagttgtt gttccagcaa tgccagtgtt ttcggcactt
    21061 tgatcagcac cacacccggc tgttgcgggt agtcggcggt gctgtcgagg aacttcacgc
    21121 tcgactcatc aatgccgttg agacgtaaat tctcacgcgt cgccagctcg ctgatgtatg
    21181 agtcaccaat gctgtagggc ttatgttccg ccagcacaca gcttaacgca ccaaaagcat
    21241 cattcaggat caacaccggg ccgcggattt ctgtgtcgtc caactgttgc agcaaatatt
    21301 catccgccgc ttcccacgcc tgtagcgggt taacgtcatc cgtcgccgga aaacgttgta
    21361 gtgtcagtga acggaaaccg ttgtctaagt ggctcatcgg ccctcctgaa tgataaaatt
    21421 tcgacgttgc ccctgaaaag ggtgcgtgag tatacccttt ttctcttttt cgtgggcggt
    21481 tatgagcaat cttacttatc ttcagggtta tcccgagcag ctactttccc aggtgcgaac
    21541 gctgattaac gaacagcgtc tgggcgatgt gctggcaaaa cgttatccgg gaacacacga
    21601 ctacgccact gataaagccc tctggcaata tactcaggat cttaaaaatc agtttctgcg
    21661 caatgccccg ccgatcaata aagtgatgta tgacaacaag atccatgtgc tgaaaaacgc
    21721 gctcggctta catactgccg tttcgcgtgt gcagggcggc aagctgaaag caaaagcaga
    21781 gatccgcgtc gctaccgtgt ttcgcaacgc gccggaaccg tttttgcgca tgatcgtggt
    21841 gcacgagctg gcgcacctga aggagaaaga gcataacaaa gcgttttacc agttgtgctg
    21901 tcatatggaa ccgcagtacc accagcttga gttcgacacc cggctgtggc taacgcagtt
    21961 gtcgcttggg caggacaaaa tctgaaaatg atgtcaggcg ttattttttc ctcctttagc
    22021 gcctaaaatc gacctcctcc ctttcgttaa ggataacgac catgctgcgc gcatttgccc
    22081 gccttcttct ccgtatttgt ttttctcgcc ggactcttaa aattggctgt ttattgttgc
    22141 ttatagcagg agcaaccctt tttattgccg atcgcgttat ggtcaacgcc agccaacaac
    22201 tgacctggag cgatgtcaac gccgttccgg cgcgtaacgt gggtttattg ctgggggcga
    22261 ggccgggtaa tcgctacttt acccgacgta ttgataccgc agcagagttg taccacgccg
    22321 gaaaagtgaa atggctattg gtgagcggcg ataatggacg taaaaattac gatgaagcgt
    22381 caggaatgca gcaagccttg atcgctaaag gcgtaccggc aaaagccatc ttctgcgact
    22441 atgccggatt ctcaacgctt gattcggtgg tgcgtgccaa caaggtgttt agtgaaaacc
    22501 atatcaccat tatctcgcaa gaatttcata accaacgcgc catctggctg gcaaaacagt
    22561 atggtatcga tgctatcggt tttaacgcgc cagacctcaa tatgaagcac ggtttatata
    22621 ctcaactgcg ggaaaaactt gccagagtca gcgcggtgat tgatgcgaaa atcctccatc
    22681 gccagccgaa atatctcggg ccgttggtta tgattgggcc gtttagtgag catggctgtc
    22741 cggcgaaaga ataatgcgta tctgcgcacg ttgaagatga aaaaggcgtg ctacattgac
    22801 gagagaatcc ctttatggag tatccacgcg ttatgatacg tttcgctgtg attggtacga
    22861 actggatcac tcgccagttc gtcgaggccg cccatgagag cggtaaatac aagttaaccg
    22921 ccgtttattc ccgcagtctt gaacaggccc agcacttcgc caatgatttt tctgtcgagc
    22981 atctgtttac gtcgctggaa gcgatggcag aaagcgatgc cattgacgcg gtgtatattg
    23041 ccagcccgaa ttccctgcac ttttcccaga cacaactttt ccttagtcac aaaattcatg
    23101 tgatttgcga gaaaccgctg gcgtcgaatc tggcggaagt ggatgccgcc attgcctgtg
    23161 cgcgggaaaa tcaggtggtg ctgtttgagg catttaaaac ggcctacctg ccgaactttc
    23221 atctgttacg ccaggcgctg ccgaaagtcg gcaaactgcg taaagtcttt ttcaactatt
    23281 gccagtattc ctcgcggtat caacgttacc tggatggtga gaatcccaac acctttaatc
    23341 cggcattctc taacggttca attatggata tcggctttta ctgtctggcg tcagcggtgg
    23401 cgttatttgg tgagccgaaa agcgtgcagg caaccgccag tttgttggca agcggcgtag
    23461 atgcccacgg cgtggtggtg atggattacg gtgatttcag cgttacgttg cagcactcaa
    23521 aagtgagcga ttctgtcctg gcaagcgaga ttcagggcga agcgggatcg ctggtgattg
    23581 aaaaactgtc tgaatgccag aaagtttgct ttgttccgcg tggcagccag atgcaggatc
    23641 tcacccaacc gcagcatatt aataccatgc tctacgaagc agagctgttc gctaccctgg
    23701 tggatgagca tctggtggat catccagggc tggcggtcag tcgcatcacc gccaaactgc
    23761 tgaccgagat ccgccgccag actggggtga tttttccggc agatagcgta aaactataaa
    23821 ttgccaaagt aaaacagtgt aaaaggtatg taacagacca ttgactgact gaatggtctg
    23881 tcatactttg ttacctgcaa aggggagtaa cttcattgcc ggtcgatcgt cattacgatg
    23941 tgtgaaaaac cacatccggt caccgggcaa cccgaaagga atacgcagac gtattccttt
    24001 tttgttgtaa gtgagacctt gccggaaggc gaggtctatg cataaaaagc agcggctgac
    24061 gtcttccgac gttggccgtt tttttatgtg taaggaactt ctatgaatac tgtcggcacg
    24121 ccgttgctat ggggcggatt cgctgttgtt gtcaccatta tgctggctat cgacctgttg
    24181 ttgcaggggc gtcgtggggc acatgccatg accatgaaac aggctgcggc ctggtcgctg
    24241 gtctgggtga cgctgtcgtt actgtttaac gccgctttct ggtggtatct ggtgcaaacc
    24301 gaaggtcgcg ccgttgccga tccacaggca ctggcctttc tcacgggtta tctgattgag
    24361 aaatcgctgg cggtcgataa cgtctttgtc tggctgatgt tgttcagcta tttctctgtt
    24421 ccggcggcat tacaacgccg cgtgctggtg tatggcgtac tcggggcgat tgttctgcgt
    24481 accatcatga tcttcactgg tagctggctg atttcacagt tcgactggat cttgtatatc
    24541 ttcggtgcct tcctgctgtt taccggcgtg aagatggcgc tcgcccatga agatgaatca
    24601 ggcattggcg acaagccgct ggtgcgctgg ctacgcggtc atttgcgcat gaccgacacc
    24661 atcgacaacg agcatttctt tgtgcgtaag aatggtttgt tgtatgccac gccgctgatg
    24721 ctggtgctga ttctggtgga gttgagcgac gtgattttcg ccgtggatag tattccggct
    24781 atcttcgccg tgaccaccga tccgtttatt gtgctgacct caaacctgtt tgcgatcctc
    24841 ggcctgcgtg cgatgtattt cctgctggca ggtgtggcag agcgtttctc gatgctcaaa
    24901 tatggtctgg cggtgattct ggtgtttatc ggtatcaaga tgctgattgt cgacttctac
    24961 catattccga tcgctgtctc gctgggcgtg gtgtttggca ttctggtgat gacgtttatt
    25021 atcaacgcct gggtgaatta tcggcatgat aagcagcggg tagaataatt tttaatctgc
    25081 ctaagccgtg tactctgtca tcaacatgag caccgttttc tccctctccc caaaaggggc
    25141 gaggggactg accgagtact tttttgacgt tgtcatcagt ctggaagccg cacgttgggc
    25201 tttattttca tgtcaaacaa atgtaaccat taagttgcga aatataactt ctctttaaaa
    25261 tccagcatat ttcgcttccc gaagctgtaa ctttccttat actcgacctt gcaaacactt
    25321 tgttacatcc tgaaagatgc gtcgacagaa cgcaccaggg atgtgcgaca acacaatgaa
    25381 aggatcgaaa aatgactacg caacattcac cggggctatt ccggcgtctg gctcatggca
    25441 gcctggtaaa acaaatcctg gccggcctta ttctggggat tcttctggca tggatctcaa
    25501 aacccgcggc ggaagctgtt ggtctgttag gtactttgtt cgtcggcgca ctgaaagccg
    25561 ttgcccccat cctggtgttg atgctggtaa tggcatctat tgctaaccac cagcacgggc
    25621 agaaaaccaa tatccgccct attttgttcc tctatctgct gggcaccttc tctgctgctc
    25681 tggccgcagt aatcttcagt tttgccttcc cttctaccct gcacttgtcc agtagcgcgg
    25741 gtgatatttc gccgccgtca ggcattgtag aagtgatgcg cgggctggta atgagcatgg
    25801 tttccaaccc cattgacgcg ctgctgaaag gtaactatat cgggatcctg gtgtgggcaa
    25861 ttggcctcgg tttcgcactg cgtcacggta acgaaaccac caaaaacctg gtcaacgata
    25921 tgtcgaatgc cgttaccttt atggtgaaac tggtgattca cttcgcaccg atcggtattt
    25981 ttggtctggt ttcttctacc ctggcaacca ccggtttctc cacgctgtgg ggctacgcgc
    26041 aactgctggt ggtactggtt ggctgtatgc tgctggtggc gctggtggtt aacccactgc
    26101 tggtatggtg gaaaattcgt cgtaacccgt tcccgctggt gctgctgtgc ctgcgcgaaa
    26161 gcggcgtgta tgccttcttc acccgcagct ctgcggcgaa cattccggtg aatatggcgc
    26221 tgtgtgaaaa gctgaatctg gatcgcgata cctattccgt ttctattccg ctgggagcca
    26281 ccatcaatat ggcgggcgca gcaatcacca ttaccgtgtt gacgctggct gcggttaata
    26341 cgctgggtat tccggttgat ctgcccacag cgctgctgtt gagcgtagtg gcttctctgt
    26401 gtgcctgtgg cgcatccggc gtggcggggg ggtctctgtt gctgatccca ctggcctgta
    26461 atatgttcgg tatttcgaac gatatcgcca tgcaggtggt tgctgtcggc tttattatcg
    26521 gcgtattgca ggactcctgt gaaaccgcgc tgaactcgtc aactgacgtg ctgttcactg
    26581 cggcagcttg ccaggcggaa gacgatcgtc tggcaaatag cgccctgcgt aactaatact
    26641 tagccccttt cgtctacggc ggaaggggtt ttctccactt taaacggatc aattcccctt
    26701 ctctgcatac gccagaaacg aatgatattc aggccattca tcagcagaaa actgccttca
    26761 atcatcgtgc cgcctatcga ccccgcccag aagttgtgaa tcacccagca acacgttgaa
    26821 aaccacatta cgcagcgcat ggtcagccct ttacagcgga acagcgccca ggtactgaca
    26881 atcgtgccga ttaccggcaa tagttcgaca ggatgatgga acttcgcgag gccaattccg
    26941 ccagtcagca caataaaaat cgccattacc cataagctgc gcgtgcgtaa ggtaatcaat
    27001 gtacgaatgg cattaaggat ggcactggca ccggcaggat aggtgcccag aagaaaaaaa
    27061 tgtacgccaa taacggcgct atagaccgaa agttgctttt tgaagcgacg ttcgtcacga
    27121 ttgaaaaatg ttgtgatacc aatcagaaag gcgatgacac ccacgccctg ggccagccaa
    27181 tacgcggtca tgataaatcc ttagcaggta tggaaaagca aacggcgctt cacattatga
    27241 aacgccgttt tttattaaca actcatttcg actttatagc gttacgccgc ttttgaagat
    27301 cgccagttcg cggaagtcgt tacgctcgtt gcaggtttgt ttaccattgg caaactcaac
    27361 gatggtgtcg ataaattctt ccagcaactg cggcatcgct ttaccgtgga tcagctgacc
    27421 cgcgtcaaag tcgatccagt gttttttctt cgccgccagt tcactgttgg tggcgatttt
    27481 caccgtcggc acaaatccac catacggcgt accacgacca gtactgaaca gcaccatatg
    27541 gcagcccgca cccgccaggg cgctggtcgc tacggcatcg ttacccggcg cacttaacaa
    27601 gttcagcccc ggcgttttca gacgctcgcc gtaacgcagc acgtcaacca cgacactggt
    27661 acccgctttc tgggtacagc caagtgattt gtcttccagc gtggtgatac cgcctgcttt
    27721 gttccccggt gatgggtttt cgtagatcgg ctggtcatgg gcaataaagt actgcttgaa
    27781 gtcattgacc atggtgacca gtttttcaaa cgttgcttcg tcgcgacaat ggtccatcag
    27841 caactgctct gcgccaaaca tctccggcac ttcggtcagt acggtagtac cgccgttagc
    27901 aatcacgtag tcagagaaac gccccagcat cgggttagca gtaataccag aaagaccgtc
    27961 agaaccaccg cactccagac caaacttcag ttcgctgagt ttgcccggct cgcgtttatc
    28021 attgcgcatc acgctataca gctgatgcaa atgcgcgatc ccggcctcga tttcatcatc
    28081 ctgctgttgg cagatcatga aatgaacgcg ttcaggatcg atatcgccca acgtttcacg
    28141 gaatgcggca acctggttgt tttcacagcc cagaccaatc accagcactg cgcccgcgtt
    28201 cgggtggcgc accatgtttt gcagcatggt gcgagtattg atgtgatcgt cgcccagttg
    28261 tgagcagccg taggtgtggc tgaagaggaa cacgccgtcg gtaccttcag cgttgttggt
    28321 ctctttcagg aaacgatttt ggatctgccg cgcgatgccg ttgacacagc ccacggttgg
    28381 gaggatccac agttcattac gcaccccgac atcgccgtta gcgcgacgat agatctgcac
    28441 ttcacgatct gccgcttgcg caggcagatc ctgaaaatca ggttgatagc gatactgatc
    28501 cagatcgctc agattcgtgc gcgtattgtg ggcgtgaacg tgttcccccg ccgcaatatc
    28561 cgccaatgca taaccaatcg gcaggccata tttaatgacg ttggcccctt ttgcgatatc
    28621 cgttaacgca aatttatgtc cacgagcaac atcctggcgc agcgtaacag tctggttatc
    28681 gacactgact tctgtgcctt cagccaaatc cgctaaagca accgcgacgt tatccagcgc
    28741 atggatcttg atgtattgca tatcaacccc agaccttagt tcagttcaat ggcgaagtag
    28801 tcacgcgcat tgttaaagca aatatttttc accatctcgc ccagcaggtt gatgtccgcc
    28861 ggtgcttcgc ctgcttccac ccagcgaccg atcatctggc acagaatgcg gcggaagtat
    28921 tcgtgacggg tgtatgacag gaagctacgg ctgtcagtca gcatgccgac aaagcggctc
    28981 agcagaccga gctgcgccag ttgggtcatc tgacgttcca taccgtcttt ctggtcgtta
    29041 aaccaccagc cggaaccgaa ctgcattttg cccggcatac cttcgccctg gaagttaccg
    29101 atcatggtgc ccagcacttc gttatcgcgc gggttcaggc agtagagaat ggttttcggc
    29161 agcaggtttt cttcattctg cttgctcagc agcttagaca gctcttccgc catcggacgg
    29221 tcgttgatgg agtcaaagcc gacatccggc cccagcagtt taaactgacg caggttgtta
    29281 ttacgcagtg cgccaatgtg gtactgctgt acccagccgc gacgtgcata ttcggcacca
    29341 aggaacacca gcaccgcagt tttgaactgt gccacttcgt gctcgctcag ggtttcgcct
    29401 gccagacggc gcgcgaggat actgtccagt tccgcttcgt tcgcttcagc aaacatcacc
    29461 acatccagcg cgtggtcaga cactttacag ccgtgagcgg cgaagtgatc caaacgttta
    29521 gtcagggcag tttgcaggtc agcaaagcgg cgaatgtcgg tatcggaaac ttcgcccagc
    29581 ttcgccatgt agtcgttaaa ggtcgcctgt tcaatgttaa aggctttgtc cggacgccag
    29641 ctcggcagca ctttaatggt gaaagagccg tctttggcga tctctgcgtg atgctccaga
    29701 gaatcgatcg ggtcatcggt ggtgccgacc attttcacgt tcatctgctg catgatgccg
    29761 cgcgcggaga agttatcctg cgccagcagt tcgttacatt cgttccagat ttcatcggca
    29821 gttgacggag aaagcaattt gccagtgata ccaaacggac gacgcagctc gaggtgcgtc
    29881 cagtggtata acgggttgcc aatagtgtgc ggaacagtcg ccgcccaggc gtcaaatttt
    29941 tcacggtcag acgcatcacc ggtgcacaga cgctcggcca caccgttggt acgcatagcg
    30001 cgccatttgt agtgatcgcc cttcaaccag atgtcataca ggtttttaaa acgatagtct
    30061 tccgcaatct gctgcggcgg caaatggcaa tggtaatcga aaatcggctg gtcttttgcg
    30121 tagtcgtgat acagacggcg ggcaaattca gtatctaaca ggaaatcttc agtcataaac
    30181 ggagtcattt tcgtcttcct ctcaacgagt gagctagctt gcttatggtg cgatgctgac
    30241 aaagttatca caccaatttc cagagtccga agatattttc gtgagttaga tcaataaacg
    30301 tagttaaaaa aattactctc aaagtggtaa atctcgctgc aggccgcgcc agcactggcc
    30361 tcgccgtcgt caggtaatgt ccctacaaat attcgcacat ttgtgatggc tctcaccttt
    30421 taaagttgta tgacaagtta tctttctgcc gtcgcaaatc ataagtcgac ggaatgcaaa
    30481 ttgccgattc attcatttgt tagatgaatc gggttaaccg gtacggaagc cgaattagca
    30541 cgaaactttc atggcaacgt tcggggcgtg ccggtttttt ttcggttacc cggtcgtaac
    30601 taacatcttc agcctctggc gggatgatgg ccgcgcttcc tgcggatata acaaaacgat
    30661 gaggttttac atgcgtaaaa ttaaagggtt acgttggtat atgatcgcac tggtgacgct
    30721 cggcaccgtg cttggttacc tgacgcgtaa cactgtggcg gcagctgcgc caactctgat
    30781 ggaagagtta aacatctcca cccaacagta ttcctatatc atcgcagcgt attctgctgc
    30841 ttatacggtc atgcaaccgg tagcgggtta tgtactggat gtgctgggta cgaaaatcgg
    30901 ttatgcaatg tttgctgtac tgtgggccgt gttctgtggt gcaactgcgc tggcaggtag
    30961 ttggggtggc ctggcggttg ctcgtggtgc ggtcggtgcc gcggaagccg cgatgattcc
    31021 ggcgggtctg aaagccagct ccgaatggtt cccggcgaaa gagcgttcca tcgcggtagg
    31081 ttactttaac gtaggttctt cgattggtgc gatgattgcg ccgccgctgg tggtatgggc
    31141 aatcgtgatg cacagctggc agatggcatt tatcatctcc ggtgcgttga gctttatctg
    31201 ggcgatggca tggctgattt tctataaaca tccgcgcgac cagaaacatc tgaccgatga
    31261 agaacgcgac tacattatta atggtcagga agcccagcac caggttgaca cagcgaagaa
    31321 aatgtccgtt ggtcagatcc tgcgtaaccg tcagttctgg ggtatcgcgc tgccgcgatt
    31381 cctggcagaa ccggcatggg gtacttttaa cgcgtggatc ccgctgttca tgtttaaagt
    31441 ttacggcttt aacctgaaag agatcgcgat gtttgcctgg atgccgatgc tgtttgctga
    31501 cctcggttgt atcctcggtg gttacctgcc accactgttc cagcgttggt ttggtgtgaa
    31561 cctgatcgtt tcccgtaaga tggtcgtaac gctgggtgct gtgctgatga ttggcccggg
    31621 tatgatcggt ctgttcacca acccgtatgt cgcgattatg ctgctgtgta tcggtggttt
    31681 tgctcaccag gcactgtctg gcgcactgat tacgctctct tctgacgtgt tcggtcgtaa
    31741 cgaagtggcg acggcgaacg gtttaaccgg gatgtccgca tggctggcaa gtacgctgtt
    31801 tgcactggta gtcggtgcac tggctgacac catcggcttc agcccgctgt tcgcagttct
    31861 ggcagtgttc gacctgttag gtgcgctggt tatctggacc gtgttgcaga acaaaccggc
    31921 gattgaggtg gcgcaggaat cacataacga tcccgcaccg caacattaag gcttcgtgta
    31981 agttttagtt tcccttcaaa gccgccttct caggcggctt tttcatcact gcgagtagag
    32041 ctaaactcgc aaaagtggta taacaaatat agtctgccgt atcatgcctg gagcgcatat
    32101 ggaaatcact gaaccacgcc gtttgtatca acaacttgcc gctgacctga aagagcgcat
    32161 cgaacagggc gtctatctgg tgggtgataa actgcctgcc gaacgcttta ttgccgatga
    32221 aaaaaacgtc agccgcaccg tggttcgtga agccatcatc atgctggaag ttgaaggcta
    32281 tgtcgaagtg cgtaaaggtt caggtattca tgtggtttcc aaccagccgc gccatcagca
    32341 ggcggctgac aataatatgg aattcgccaa ttacggtccg tttgagttgc tccaggctcg
    32401 ccagctcatc gaaagtaata ttgccgagtt cgcggcaact caggtcacaa aacaggacat
    32461 catgaaattg atggccattc aggagcaggc acgcggtgaa caatgcttcc gtgattccga
    32521 gtgggatttg cagttccata ttcaggtagc cctggcgacg cagaactccg ccctggcggc
    32581 tatcgttgaa aaaatgtgga cccagcgtag tcataacccg tactggaaaa aactgcacga
    32641 acacattgat gcccgtaccg tcgataactg gtgtgatgac cacgatcaaa tcctcaaggc
    32701 gctgattcgc aaagatcctc atgctgctaa gctggcaatg tggcagcacc tggaaaacac
    32761 caagatcatg ttatttaacg aaaccagcga cgatttcgag ttcaatgccg accgctatct
    32821 gttcgccgaa aacccggtgg tgcatctcga tacagccacc agcggcagta aatgaaattc
    32881 ctgtccgaca ggcgctacag tgcgcctgtt tgtctcgtcg gtaagcgaaa agtataaagt
    32941 gtcagcctgt gtaaatcctc tcgcctccct cccctgcatt cagcaaaatc agactccacg
    33001 gacatgtaat tttgataacg aacaacgttg acctttgtta caattagatt caatttgaat
    33061 ttatgctttt gaatgctttc ttatctcacg atttaacagg gaatagttca ggctgtgttg
    33121 atgtatcaaa cccgcagaac ataccaagac agcaataaca ttgcggtagt gcatcattta
    33181 aaaccagcgt ggcgttaacc gattcaccag gaataatgaa tggaactttt gacccaattg
    33241 ctgcaagccc tgtgggcgca ggattttgaa accctggcca atccgtcgat gattggcatg
    33301 ttgtattttg tcttgtttgt cattttgttc cttgaaaacg gcttgcttcc ggcggccttt
    33361 ttaccgggcg acagtttact ggtactggtc ggcgtattga ttgcgaaagg cgcgatgggc
    33421 tatccgcaaa cgattctgct gctgaccgtt gccgccagcc tcggttgctg ggtcagctat
    33481 attcaggggc gatggctggg caatacccgc accgtacaaa actggctatc tcatttaccc
    33541 gcgcattatc atcaacgcgc acaccatctt tttcataaac acggtttatc ggcgctgtta
    33601 attggtcgct ttattgcgtt tgtcagaaca ctgctgccga cgattgccgg gttatcaggg
    33661 ctgaataacg cgcgctttca gttcttcaac tggatgagtg gtctgctgtg ggtgttgatc
    33721 ctgacaactc tgggttacat gctcggcaaa acgccggtat ttttaaagta cgaggaccaa
    33781 ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg tgtttggcct ggcaggttct
    33841 ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt aagggatgca aatacctcgc
    33901 atgtcacttc gccagctagc ctggtccggc gctgttttac ttctggtcag cacactattg
    33961 ctggcctggt ccgcggttcg ccagcaagag tctacgctgg cgattcgtgc cgttcatcaa
    34021 gggacaacga tgccagacgg tttttcaatc tggcatcacc ttgacgctca cggcattcct
    34081 ttcaaaagta tcacccccaa aaacgacact ctgttaatta cctttgactc cagcgaccag
    34141 agcgccgccg caaaagcggt cctcgacaga acattgcccc atggctacat cattgcgcag
    34201 caggacaata acagtcaggc tatgcagtgg ctgacccggt tgcgggataa ctctcatcgc
    34261 ttcggataac ttcctgttat ccgaaacaga tcactcactt tggtgatttc accgtaactg
    34321 tctatgatta atgaggcggt ggttaacacg accgcttcgt tgtctggaca atcgttcctt
    34381 tgtaataggt ccgaatcaca atggaaggtt caagaatgaa ataccgcatc gctttagctg
    34441 tttctctctt tgctcttagt gccggtagtt atgccactac cctgtgtcag gaaaaggagc
    34501 aaaatatcct taaggagatc agctatgccg aaaaacacca aaaccagaat cgtattgacg
    34561 gtctgaataa agccctgagt gaagtccggg ccaactgttc agatagccag ctgcgtgccg
    34621 atcatcagaa gaaaatcgca aagcagaaag atgaggtggc ggaacgccag caagatttag
    34681 ccgaggcgaa gcaaaaaggc gatgcagata agattgccaa acgcgaacgg aaactggcag
    34741 aagcgcagga agagctgaaa aagctgaaag cgcgcgacta ctaactcaca atagtcacta
    34801 cttactcacc tggagaaaac tatgtcgaaa gaacacacta cggaacatct gcgtgctgag
    34861 ttgaaatccc tttccgatac gctggaagag gtgcttagct catctggcga gaagtcaaaa
    34921 gaagagttga gtaagattcg taacaaagcg gagcaggcac tgaaacagag ccgttatcgc
    34981 ctgggtgaaa ccggtgatgc cattgccaaa caaacccgtg tcgcggcggc gcgtgccgat
    35041 gagtatgtgc gtgaaaatcc gtggacgggc gtgggcattg gcgcagcaat cggtgtagtg
    35101 ctcggcgttc tgctgtcgcg tcgttaatta tggcggacac tcatcacgcg caagggcccg
    35161 gtaaaagcgt tctgggcatc gggcagcgaa ttgtttccat catggttgaa atggtggaga
    35221 cacgtctgcg gctggcggtg gtggagctgg aagaggaaaa agcgaatctc tttcaacttt
    35281 tactgatgct gggcctgacg atgcttttcg ctgcatttgg tcttatgagc ctgatggtgc
    35341 tagttatctg ggcggttgat ccgcaatatc gcctgaatgc gatgattgcc accaccgtgg
    35401 tgttgctgct actggcactg attggcggca tctggacgtt acgtaaatcg cgtaagtcta
    35461 cgttgctgcg ccacacgcgc catgagctgg caaacgatcg gcaactgctc gaggaggagt
    35521 cccgtgagca gtaaagtcga acgtgaacga cgtaaggcgc aactgcttag ccagatccag
    35581 caacaacggc tggatctttc cgccagtcgt cgtgaatggc tggaggcaac aggcgcttac
    35641 gatcgtcgct ggaatatgct gttgagtctg cgctcctggg cgctggttgg cagtagcgtg
    35701 atggctatct ggacgattcg ccatcctaat atgctggtcc gctgggccag acgcggtttt
    35761 ggtgtatgga gcgcctggcg tctggtgaaa acaaccctca atcaacaaca gcttcgcggt
    35821 taacttcctc tctggccgga gaaattccgg ccttatccct caaatttttt gaaaatattt
    35881 gacagttttc cttgctaaca atcacgcttc gtcacgttta tgattctctc catcgacagc
    35941 aacgacgcta atagcgcgcc cttgcacaaa aaaacaatca gcagcctgag cggcgcagtg
    36001 gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg tagcgcgtat tttaatgccg
    36061 attctgttta ttacggcagg ctggggaaaa attactggct acgcgggtac ccaacaatat
    36121 atggaagcaa tgggcgtccc cggctttatg ctgccgctgg tgattctgct tgagtttggt
    36181 ggtggtctgg caatcctgtt cggtttcctg acacgtacca cagccctgtt tactgctggc
    36241 tttacgctgc tgacggcatt tttatttcac agcaactttg cagaaggcat gaactcgctg
    36301 atgtttatga aaaacctgac tatttctggc ggattcctgc tgctggcaat taccggtcca
    36361 ggcgcattca gcatcgaccg cctgctgaat aaaaagtggt aagcacgcgc tatacttaac
    36421 gataaaaaac gagggggaag ctcctcgttt ttgctattgg aggatagaaa aatgggtcaa
    36481 ctgattgacg gcgtctggca tgacacctgg tacgatacca aatccaccgg cggtaaattt
    36541 caacgttcag cttccgcatt tcgtaactgg ctcactgccg atggcgcgcc aggccccact
    36601 ggcaaaggcg gttttgccgc agagaaagat cgttatcatc tctatgtttc actcgcctgc
    36661 ccgtgggcac accgcacgct gatcatgcgt aagctcaaag gactggaacc gtttatttcc
    36721 gtttccgtag tgaacccgct gatgcaggaa aacggctgga cctttgatga cagttttccg
    36781 ggagcaaccg gcgacacgct ctatcaacat gaatttctgt atcagcttta tctccacgcc
    36841 gatccacact acagcgggcg agttactgtt cccgtgctgt gggacaaaaa aaaccacacc
    36901 atcgtcagca acgaatcagc agaaatcata cgcatgttca ataccgcgtt tgatgcgctg
    36961 ggtgcgaaag ctggagatta ctacccacca gccctgcaac cgaaaattga cgaacttaac
    37021 ggctggattt acgacaccgt taacaatggc gtgtataaag ccggttttgc caccagccag
    37081 caagcctacg acgaggcggt ggcgaaagtg tttgaatcgc tggcgcgact ggaacagatt
    37141 ttaggtcagc accgttacct gaccggcaac cagctaaccg aagccgatat tcgcctgtgg
    37201 accacgctgg tgcgttttga tccagtgtat gtgacccact tcaagtgtga taagcaccgc
    37261 atcagcgatt acctgaatct gtatggcttc ctgcgcgata tctaccagat gccgggaatt
    37321 gccgaaacag tcaatttcga tcacatccgt aatcattact tccgcagcca taagaccatc
    37381 aaccctacgg gaattatttc aattggtccg tggcaggatc tcgatgaacc gcatggacga
    37441 gatgttcgct tcggttaaaa aaaggcacct tacgaggtgc ctctgataat tagctaagta
    37501 cgtgttgttc atgtcggatg cggcgtaaac gccttatcaa acctacaaaa catttatatt
    37561 caatatgttg caatgaccgc gcaggccaga taagcgtagc gcatctggca attttgcgct
    37621 tgcccttatc cttcacagcg ccttattaat tcatcgcgat aatatttact gccatttcat
    37681 tcacgtctat tcttaatttg ctgctttaaa aacaagtgat tgagcaaaat tgaggcaaaa
    37741 atggactggt atctgaaagt actaaaaaat tatgtcggtt tccggggtcg tgcgcggcgc
    37801 aaagagtact ggatgtttat tctggtcaac atcatcttta cgttcgtgct ggggctactg
    37861 gataaaatgt taggctggca acgtgccggt ggcgaaggca tcctgacgac aatctacggt
    37921 attctggtgt ttttaccatg gtgggcagtt cagttccgcc gcctgcacga caccgaccgc
    37981 tcggcgtggt gggcactgct attcttaatc ccgttcatcg gctggcttat tatcatcgtc
    38041 tttaactgcc aggcgggtac gccaggcgaa aaccgctttg gaccagatcc gaaactggaa
    38101 caagaataat aattaagcac cttgtaaaag gtgcttaatc gattattttc cgctaaaaag
    38161 tttgggaatt tcccgcagac accaggattt cgcctccccc atactgtcac gacgccaggc
    38221 cataataata tcgatctcgc tggtcgattc cgggctgacg acacgcaacc gcccttccgc
    38281 aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg ccagtaatgc
    38341 ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca gcaactgtac
    38401 ggtcaacacc gggcgctcac gagcggtatc cgccaccgca attccacgat atttcacgcg
    38461 cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatccggcg cggcaacata
    38521 gacgttcatt aaggtataga gtttgcgcga gttgatctcc gacgaggaac gaaaatgcat
    38581 atccggcgcg ataacgatat ccgcccgccc ctgctccagc cgttcccacg cccccgccag
    38641 cacttcggtg ataattgcca gttgggtatt ggcttttgcc gccagtttgt cgattaacgg
    38701 gaaaaaggca ggtgtcggta ccagcgcctc ggtcacaatg gtgagatgcg tttcccaacc
    38761 gcgcgcgagg gcttccgcat cggtagtcag tttatctgcg gcttccagca aaacgcgccc
    38821 tcgttccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg agcggtcaaa
    38881 cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg cggaaggcac
    38941 gcgtcccagc tcatccgccg ccgccgcaaa actgccccgg cgatcgatcg catccataac
    39001 ccgtagtgct tccagcgtta atgccctttc tttggccatt tcgttctcat tcaggaaatt
    39061 tgaacatacc gggcagaata tctggctaac aatgtagcgt ccagcccctt accataaaag
    39121 gaagtaaaga gaggtcaaga attatgatta ctacccgaac tgccaggcag tgtggacaag
    39181 cagactacgg atggttgcag gcccggtata ctttttcctt tggacactac ttcgacccga
    39241 aattgttagg ctatgcctcc ctgcgcgtgc ttaaccagga agtgctggcc ccaggtgccg
    39301 cctttcagcc gcgaacctat cccaaagtcg atattttaaa tgtgattctg gatggagaag
    39361 ctgagtatcg cgacagcgaa ggcaatcatg ttcaggccag cgccggtgag gtgttgctgc
    39421 tctctaccca gccgggtgtc agctatagcg aacacaatct cagcaaagac aaaccgttaa
    39481 cgcgaatgca gctttggctg gatgcctgcc cgcagcgaga gaatccgctg attcaaaagc
    39541 tggcgcttaa tatgagcaag cagcaattaa tcgcctcgcc agaaggtggg atgggaagcc
    39601 tgcaattgcg ccagcaagtg tggctgcacc atatcgtgct cgacaaaggc gaaagtgcga
    39661 atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat tcacgggaaa tttcatgcgc
    39721 ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga tggggcgttt attcgtgacg
    39781 aggctaacat tacgctggtt gccgattccc cactgcgcgc tttgctgata gatttgcctg
    39841 tctagttgtt tttacaggga gatgatgatg agtaaaaaat cggccaaaaa gcgccagcca
    39901 gtgaagcccg tggtggcgaa agaacctgct cgtaccgcca aaaattttgg ctatgaagag
    39961 atgttgagcg agctggaagc catcgtcgcg gatgctgaaa cgcgtttagc cgaagatgaa
    40021 gctaccgcgt aaagacaagt tgccggatgc ggcgtaaacg ccttatccgg cctacaaaac
    40081 ccaacaacat caacaaattg tgcaattcgt aggccagata agacgcgcca gcatcgcatc
    40141 cggcacaata cccaaaacct gacgtttatc tggccttgct cgccataatc tcgataatct
    40201 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
    40261 caacatcatg cgccacaatc ccttcattac cggttacggc ggtatcatcc agcgccatta
    40321 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgatg
    40381 caccatcgca aatcatgcca ctgacatcgc cgatcatact gctgatcgcc atcgagatgg
    40441 tttcataacg cccatccacc agccatgcca tcccggcagc ggcccccatt gctgcggtcg
    40501 ttgcggcaca cagcgcagac aaacgcggta actggttatg gatgtaaatc gcgctcaaat
    40561 gcgaaagcat cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca
    40621 ccaccacggg cattgttgcg gtgatcccct ggttacccga gccggagtta ctcattgccg
    40681 gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg
    40741 aagagagatc tttcgccagc aaaccgcgcg cgcactgttt ttccagcgtc gcaccaatat
    40801 gcagccccca gttaccgctc aaaccttcct gcgataacgc acaatttaac ttcgcagaat
    40861 cgagaataaa gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca
    40921 gcgtcgtcct ggaaagcacc gtcagcggcg actcttgctc gccctctgtc acacacgcct
    40981 gctgggtaaa caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga
    41041 cgatggtgac acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt
    41101 catcgcaagg ttcctggatc ttaactgaga ctttccccgc cgccagcagt gccttggcat
    41161 cagaaattgc ctgcgcagtt gcgtctttca gcacttccag cccggcgtta gcatttccac
    41221 ctaacgctcc cagcgccgcc gcaattggca gccccaccat tcctgtaccg ggaacggtga
    41281 cacccagacc gttcttcatc agatttggag aaacccaggc ttctacacgt tcaaccggac
    41341 cttccagttc agccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacacc
    41401 ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta
    41461 aagtcgaatc aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga
    41521 acggagaaac acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt
    41581 atttgtgcaa tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc
    41641 cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg ggcgttcagt acgatggcgc
    41701 tccaggccag caaaatggcg aaaatcatga tgccgcgctg aacgagattt tcgttaatct
    41761 tctcagcagg tatcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa
    41821 agcctaaata gacgccaaag aatgccgtca ttacagcaaa gatattgaga atgacgctga
    41881 ctactttcac ccacgctgcg ccatcaccgc taataaactg tgcggcaatc gccagcgcgg
    41941 aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca
    42001 cggcgtagaa aaagacggta acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg
    42061 ctttatgccg cgccacttca attgattttt cccgcgaacg ataagagatc accatcgggc
    42121 ttaacgtctg gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt
    42181 tcaccagcag ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca
    42241 tcgacacgcc cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt
    42301 tgaataacaa tttctcgccg cgtgaggaga tcgccaccag aatgcaaatc agtaccagac
    42361 cataaaaggg actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc aggtaggaag
    42421 cactatcgtt ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat
    42481 agagtgcacc taacaggatg ccccagtttt tacctaaata accgctgatg acgctcgggt
    42541 aatctttaca ctccggtgat tctgccagcg tattaataaa caaccgctga aacagataca
    42601 ttgccgggta accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct
    42661 gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc
    42721 cggtgtcagt actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac
    42781 gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt
    42841 ctcgctcaat attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg
    42901 gtctacgttt ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa
    42961 aatcttcaca atca
//
LOCUS       DAZKHK010000027        41610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKHK010000027 DAZKHK010000000
VERSION     DAZKHK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.027"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..336)
                     /locus_tag="ACHOS5_001507"
     CDS             complement(46..336)
                     /locus_tag="ACHOS5_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776336.1"
                     /translation="MKIELITTKQFIEQAECYFRNYMDGLRRNAPDDFCYFLNNKYNM
                     NDIMESIIKKTRYHFYDDTEEDQRNRIYGEVSHCKVKQHLRQLWIIYKCVYR"
     gene            complement(418..636)
                     /locus_tag="ACHOS5_001508"
     CDS             complement(418..636)
                     /locus_tag="ACHOS5_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9776337.1"
                     /translation="MKENSETSVKRFIRVPDVLNRVGFSRTTLYERIKEGNFPDRVKI
                     GPRCVAFVESEIDEWIEAAIRNSRQNAA"
     gene            complement(853..1689)
                     /locus_tag="ACHOS5_001509"
     CDS             complement(853..1689)
                     /locus_tag="ACHOS5_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFP9776338.1"
                     /translation="MKRSEIRKALEAWFDVERYEAIEKLTLQHFYVEVERRILAYRML
                     LSRNTIPTFNRLMLDDYRNKILSGEIFFSGDTATLGHELARTYAVNPTTRSHAQFYAK
                     TLALTEATPELSGLSQSEFLSEYLKETSLNNLSRITVDIHLEEASTEEIIEHLKVLIP
                     QWKRQLKMTSPVPREYRFGKSTFRKIIEYRLIPMMDLIFWGEDNGIKIPLSLISSLLH
                     EDSDNDRDEGMLKATDYPLAMAFLTDASYLKSLEDYMVENNYLKDSPVEKHVEDDRNK
                     KK"
     gene            complement(1968..2057)
                     /locus_tag="ACHOS5_001510"
     tRNA            complement(1968..2057)
                     /locus_tag="ACHOS5_001510"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2021..2023),aa:Ser,seq:cga)
     gene            2151..2948
                     /gene="mtfA"
                     /locus_tag="ACHOS5_001511"
     CDS             2151..2948
                     /gene="mtfA"
                     /locus_tag="ACHOS5_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP9776339.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVAIAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDPFSATNVH"
     gene            3049..3124
                     /locus_tag="ACHOS5_001512"
     tRNA            3049..3124
                     /locus_tag="ACHOS5_001512"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3082..3084,aa:Asn,seq:gtt)
     gene            3286..4548
                     /locus_tag="ACHOS5_001513"
     CDS             3286..4548
                     /locus_tag="ACHOS5_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9776340.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLRIKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKTLRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4742..6046)
                     /gene="ybtS"
                     /locus_tag="ACHOS5_001514"
     CDS             complement(4742..6046)
                     /gene="ybtS"
                     /locus_tag="ACHOS5_001514"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFP9776341.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLVVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(6074..7354)
                     /gene="ybtX"
                     /locus_tag="ACHOS5_001515"
     CDS             complement(6074..7354)
                     /gene="ybtX"
                     /locus_tag="ACHOS5_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFP9776342.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(7347..9149)
                     /gene="ybtQ"
                     /locus_tag="ACHOS5_001516"
     CDS             complement(7347..9149)
                     /gene="ybtQ"
                     /locus_tag="ACHOS5_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFP9776343.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGAASGEWVHE"
     gene            complement(9136..10848)
                     /gene="ybtP"
                     /locus_tag="ACHOS5_001517"
     CDS             complement(9136..10848)
                     /gene="ybtP"
                     /locus_tag="ACHOS5_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFP9776344.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            11105..12064
                     /gene="ybtA"
                     /locus_tag="ACHOS5_001518"
     CDS             11105..12064
                     /gene="ybtA"
                     /locus_tag="ACHOS5_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFP9776345.1"
                     /translation="MMESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            12255..18362
                     /gene="irp2"
                     /locus_tag="ACHOS5_001519"
     CDS             12255..18362
                     /gene="irp2"
                     /locus_tag="ACHOS5_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFP9776346.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLLFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLCEVRNTRRRM
                     IVPATRWHDFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQSAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYQYPQQLPPPLPVTYRDYLQQPAIQSLNA
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            18450..27941
                     /gene="irp1"
                     /locus_tag="ACHOS5_001520"
     CDS             18450..27941
                     /gene="irp1"
                     /locus_tag="ACHOS5_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFP9776347.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDAALFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDRRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLMPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPNAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKALAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDSEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            27938..29038
                     /gene="ybtU"
                     /locus_tag="ACHOS5_001521"
     CDS             27938..29038
                     /gene="ybtU"
                     /locus_tag="ACHOS5_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFP9776348.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            29035..29838
                     /gene="ybtT"
                     /locus_tag="ACHOS5_001522"
     CDS             29035..29838
                     /gene="ybtT"
                     /locus_tag="ACHOS5_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFP9776349.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            29842..31419
                     /gene="ybtE"
                     /locus_tag="ACHOS5_001523"
     CDS             29842..31419
                     /gene="ybtE"
                     /locus_tag="ACHOS5_001523"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFP9776350.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            31550..33571
                     /gene="fyuA"
                     /locus_tag="ACHOS5_001524"
     CDS             31550..33571
                     /gene="fyuA"
                     /locus_tag="ACHOS5_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFP9776351.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            34222..34971
                     /locus_tag="ACHOS5_001525"
     CDS             34222..34971
                     /locus_tag="ACHOS5_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFP9776352.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            34898..37020
                     /locus_tag="ACHOS5_001526"
                     /pseudo
     CDS             34898..37020
                     /locus_tag="ACHOS5_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(37190..38242)
                     /locus_tag="ACHOS5_001527"
     CDS             complement(37190..38242)
                     /locus_tag="ACHOS5_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP9776353.1"
                     /translation="MFLVSLLRRIAFSYYDYKTYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRAIELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            38557..39873
                     /gene="shiA"
                     /locus_tag="ACHOS5_001528"
     CDS             38557..39873
                     /gene="shiA"
                     /locus_tag="ACHOS5_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP9776354.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            39975..41429
                     /gene="amn"
                     /locus_tag="ACHOS5_001529"
     CDS             39975..41429
                     /gene="amn"
                     /locus_tag="ACHOS5_001529"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP9776355.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         9501 a        11809 c        10972 g         9328 t
ORIGIN      
        1 tcctctttag tttaatattg gaatagcaaa atatttgtat gagcattagc ggtatacaca
       61 cttatagata atccaaagct gacgaaggtg ttgttttacc ttacagtggc ttacttctcc
      121 ataaatccga ttcctttggt cttcttctgt gtcatcgtaa aaatgatatc gcgttttttt
      181 aatgatactt tccataatgt cattcatatt gtatttattg ttgagaaaat aacaaaaatc
      241 atctggtgca ttcctccgca atccgtccat atagttcctg aaataacatt cggcttgttc
      301 aataaactgt tttgtcgtaa taagctctat cttcatattt ttctccatta ttgttaaggg
      361 taataagatg ccctgagcga acagatatct gttcgacacg agatcttgtt tctgttttta
      421 tgcagcgttc tggcgtgagt ttcgaatagc cgcttcaatc cattcatcaa tctcactttc
      481 aacaaaagcg acacacctcg gacctatttt tactctgtct ggaaagtttc cttccttaat
      541 acgttcatat aaagtcgtgc gactaaatcc cactcgattt aaaacatcag gaacgcggat
      601 aaatcgtttt acgctggttt cactattttc tttcattgtc tgtcctctat tattgtaagt
      661 aatctctatg agatttcgct tttctctatg ttgtttcgac gtgaacaatg atagcgattt
      721 tgcttatggt aatttagggg cgttcataaa agagttttat gaaaaaatag aagggaggtg
      781 aatggaaaaa gattcgttaa tgctgaatgc gatcacgttc gcacggagcg aacgtaaccc
      841 tttttacttg gcttattttt tcttattcct gtcgtcttct acatgctttt caacagggga
      901 gtctttcaag tagttgtttt ccaccatgta atcttcaagc gacttcagat agctcgcatc
      961 cgtcagaaaa gccattgcta acgggtaatc tgtagccttg agcattcctt catcacgatc
     1021 gttgtcgctg tcctcgtgaa gcagcgagga aatcagtgaa agcgggattt taatgccgtt
     1081 atcttctccc cagaagatca ggtccatcat cgggataaga cgatattcaa taatttttct
     1141 gaacgtgctt ttgccaaaac gatactctct tggaactgga gacgtcatct taagctgcct
     1201 cttccattga gggatcaaca ctttcagatg ttcaatgatc tcttcggttg atgcttcttc
     1261 cagatgaata tcgacagtaa tacgtgaaag attattaagg gatgtctctt tcagatactc
     1321 ggacagaaat tcagactggc ttaacccaga gagttcaggc gtggcttcgg ttaatgccag
     1381 cgttttcgca taaaattgcg cgtggcttcg tgtcgtagga tttactgcgt aggttctggc
     1441 aagctcatgt ccaagagtag cggtatcacc actgaaaaag atttcaccgc tgaggatctt
     1501 gttgcggtaa tcatccagca ttaatcggtt aaaggtaggt atggtgttcc ggctcaacaa
     1561 cattctgtaa gccaagatgc gccgctcaac ctcaacataa aaatgctgta aggtgagttt
     1621 ctctatcgcc tcatagcgtt caacatcaaa ccatgcttcc agtgctttcc tgatttcact
     1681 tcttttcatc catcctccaa caatccattt cctttgatat atcttcacag gatccgtttg
     1741 gaacaattga agttcaactg ttttgtcatc agagacgggg gaatggctaa gttgttgtta
     1801 agagtgtgaa ctcgagagca taaaagtgga tgcccgacgc gcggcaaaat ggtaaaaaac
     1861 agcatcagta gtaagaaaaa ttgagcgaga gaaaggaatt ttaatgcagg gatttatgca
     1921 tgacaaaatc aaaaatgctt tgtaagtgat tgattttgtt gaatgattgg cggagagagg
     1981 gggatttgaa cccccggtgg agttgccccc actccggttt tcgagaccgg tccgttcagc
     2041 cgctccggca tctctccgtt cagatggttg ccatgatgcc aggaaatttg gcattttaac
     2101 agtccctgtc cgtgcaattt tgttcaagtg acgagtttgc gagcaaaacg atgattaagt
     2161 ggccctggaa agtacaagaa tcagcacatc aaactgccct tccctggcag gaagcactat
     2221 cgatccccct tttaacgtgt ctgacagaac aggaacaaag caaattggtc gctattgccg
     2281 aacgtttttt acagcaaaaa cggcttgttc ctttacaggg ctttgagctg aattcattaa
     2341 gaagctgccg gatagcactt ctattttgcc tgcccgttct ggagttagga ctggaatggc
     2401 tggatggttt tcatgaagtc ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg
     2461 aagacgatat cggtctggtg cataaccaac gtattgttca gtcaggtcag agctggcagc
     2521 aagggcctat cgttttgaac tggttggata tacaagattc ttttgatgct tctggtttta
     2581 acctgattat tcatgaagtc gctcataagc tggacacccg taacggcgat cgcgccagcg
     2641 gagttccctt tatttcgttg cgtgaggttg ctggctggga acacgatctt catgctgcaa
     2701 tgaacaacat tcaggaagaa atcgaattag ttggtgagaa tgcggcgagc attgatgctt
     2761 atgctgccag tgatcctgct gaatgttttg ccgtactttc tgaatatttc tttagcgccc
     2821 cagaactttt tgctcctcgt ttcccttcat tgtggcaacg tttctgtcaa ttttatcaac
     2881 aagatccttt gcagagactg catcacgcta atgatacaga cccgttttcg gcgacgaatg
     2941 ttcattaatt aacaactttg cagattaatt aaccaattga aatggcttat gaaatttagt
     3001 gttgacagac aaggtaccgc taagtaatat gcgccccgtt ctcacgattc ctctgtagtt
     3061 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
     3121 gccaattttc tgttttcatg catccttgcg aatctttatc tgatgttgat tcaacaggtt
     3181 agtgtgaaaa ctcttctcgg tgcattttga ttttacccta cgcatcggga aaaattggtg
     3241 gtcaaatctg gggtcaggtt agttcgataa tggagtgacc cccatatatc ccttaccgac
     3301 gcaaaaatcc gcaccctcaa gccttctgat aaacccttta aagtctccga ttctcacggt
     3361 ctgtatctgc ggatcaagcc gggtggctcc cgccactggt atctcaaata ccgtattagc
     3421 ggtaaagaat cccgcattgc gctgggcgcc tatccagcca tctccctgtc tgatgcgcga
     3481 cagcaacgtg aaggtatccg taaaatgctg gcgctgaata tcaacccggt acagcagcgg
     3541 gctgctgtac gtggctcacg aacaccggag aaagttttta aaaacgtggc gctggcgtgg
     3601 cataaaagta acaggaaatg gtcgcagaac accgccgacc gtctgcttgc cagcctgaac
     3661 aatcacatct ttccggtcat cgggaaccta cctgtatcag aacttaaacc ccgtcatttc
     3721 attgacctgc tgaaagggat cgaggaaaaa ggtctgctgg aggttgcgtc ccgcacacgg
     3781 cagcacctga gtaacataat gcgccatgcg gtccatcagg agttaatcga tacgaaccct
     3841 gcagcaaacc ttggcggcgt gaccacacct cctgtcagac ggcactatcc tgccctgccg
     3901 ctggagcggc tgcctgaact gcttgaacgt attggggcat atcatcaggg ccgtgaactg
     3961 acccggcatg ccgttctgct gatgctgcat gtgttcattc gctccagtga actgcgtttc
     4021 gcccgctggt cagagattga tttcacaaac cgagtctgga cgatacccgc gacgcgagaa
     4081 cccattattg gcgtgcgtta ttccggccgc ggggcaaaaa tgcgaatgcc gcatatcgtc
     4141 cccctctcag aacagtccat cgccattctg aaacagatta aggatatcac cggtaataat
     4201 gaactgatct tccccggcga ccataacccg tataagccaa tgtgtgaaaa cacggtcaat
     4261 aagacactgc gggtgatggg ttacgacacg aaaaaggata tctgcggtca cggcttccgg
     4321 gcaatggcat gcagtgcgct gatggaatcg ggtttatggg caaaggacgc agtagaacgc
     4381 cagatgagtc atcaggagcg caataccgtg cgcatggctt atattcataa ggcagagcac
     4441 ctggaagccc gcaaagcgat gatgcagtgg tggtcggatt atctggaagc atgccgagaa
     4501 tcttatgcac cgccttatac aattggtaaa aataagttta tcccatagta tactaacgaa
     4561 tcccacactc tggcaaatta ccaccacctc cacaagaggt ggttctatat agacttttat
     4621 caacttcatg acgaaaaaca cctattttat cccaaaagaa gacaatagag tcacagagga
     4681 atcaaagagc gactcaattg tctttggaca gtctggttgt gaggttagaa aacagttact
     4741 cctacaccat taaatagggc gcaatgctcg ctaatttctc ccgggtttct gtcagttcgc
     4801 gttccggcgt agattgcgcg atgattcccg cccccgcctg tatccagcag cgctggctat
     4861 cctgaaatac ggaacgcaga actagcgccg catcaaagcg cgtatcgtcc agcagcagga
     4921 ttgcgccgga ataaagctct cgcggcgtct tctcaatttg cataatcgcg ttcagagcag
     4981 catttttagg gatacctgag gcggtaatcg acggaaacag cacggtaaac gcatcccagg
     5041 catccttgtt ttccgcaagc tgaccgctca cgccagaccc cagatgctga acgctgccgc
     5101 gctggcgaac cgacattaaa tcttcaacca ccacgctgcc cggctggcaa acggcctcca
     5161 gttcagcaat agcttctttg acagaaagga tatgctcaag cacctcttta ctgtcgtgca
     5221 gcagttctgc ctctttcgcc ttattatgct ccgggtttcc catgcgatcg cgggtgcccg
     5281 caagcggttc agtgaccact ttattgcccg tcactgacat caccagttcc gggctaaagc
     5341 ccagcgcttc gcgtccttcc tgacggaaca taaacgagcg cacaggcgtg tttgcctgcc
     5401 gcccgtataa cagcgtggcg ggcatatcaa tccgcgatgg caggggaatg gcgcgcgaga
     5461 caatcacttt gacatactcg ccacggcgga tctccgcaac ggcgcgcgca acctgttgtt
     5521 tatatgcctc accattgagg gcggtatcca ccaccagtgg tgcgttctgc gtcgttgtac
     5581 cggcctcttt tacccactcg cacaaacgtc ggcacccgtc ggcggagtcc gcataaacgg
     5641 tgacatttcc cttttcaaaa ataagttctt cacggggaac ggttaacgtc agcagcggcc
     5701 actccccggc gttaaaggca atcccccgcg catgcgccgc aaagttgaac ccaacctgtc
     5761 catatacccg acgtcctttc acctgaggat gcgccataaa acgacgcgcg cagtcggcaa
     5821 tggaatccac cgcccatttt tgctccccgg catcatcaat aaatgtcgcc tgcgttccgt
     5881 cggcattaat gtgcagccgt gcctggcacc ctttgcctaa ataccaacag ggttgacgct
     5941 catagacata acactcttct tctgcgaact ggcgtaaaac gccagaaatc gtcggtagcc
     6001 attgttcctc tggtaacgcc agatgtagaa attcactgat tttcatccct tacctctctg
     6061 tgttattcgc ggatcaatca attgcgcttg tcatcagttc tcttgccgaa tgcagccgga
     6121 tataagcaac ccacgccgcc agcaacgtga atgccccggc aaacaggaag caggctgcat
     6181 agccaaaatg ttgagcaaca acgccaccga taacgcctgc cagaatggcg attgccgcat
     6241 cagtacattg aaagagggtg aagtcgacac cggcctgcaa aggcgatgaa agcgacatca
     6301 gcgtggcgta aagacagacc agcgcgcagg ccagcgaaat ggactggaca atgaccagac
     6361 actggagaat ctgcagcagc agatgacccg gcgccatcat gagcgtcatc accaccgcca
     6421 gcagcgcgat cccctggacg ccataagccg tcaacagcgc tctgccgggt gaggtgtatt
     6481 tcatcagcaa tccgccggcc agcgttcctg ctatgcccgc tgcaatattg ccgccgctga
     6541 acagcgcgcc caatgcggac atgctcaacc catgatccaa caacagcggt gccagaagag
     6601 gcagcacaaa ccgcatgcct gaattcagca tcaataccag cagtaagccc aggcgcgcct
     6661 gcttcctcct tagcgcataa cctaacgccg gaacatgcgg gatagtcgct gttcgcgtcg
     6721 gctccgtaat gcgccacagc gggagtgaca gcgccataat cagcaccgcc atcatcagca
     6781 tggcgacagg ccagccggat gccgccgaca gcatcaggaa caccccgccg ccgcacatca
     6841 ttcccagata gctgccgccg acctgcacgc tgtttcccca gccgtaaccc gtgcgggtca
     6901 gttgatcgac acaaaagccg tcgctggcga tatcaatggt ggaggcgacc gtgcctgcca
     6961 ccataaacag aacaaataac gcgacgattt gcgtatcggg gaatccccct tctcgcccaa
     7021 accagccaat cgctgcggca atagtcagga tcgccgctag cgcgacctga ccacgcagga
     7081 ttaacatgcg ggaacggcgt tcctggctac cgggcggaag ccgccagcgc tcgatccacg
     7141 gcgcccaaat aaacttcagc gcccagggca gcataaacag ggttgtcgca ccggcaagcg
     7201 ccagcgaacc gccagcagcg cgcaccagcg cgggtaagga ctgcatagac agcgccgata
     7261 ccagactctg cgtcacatag acgccggcaa tagcaaaaat cacccgcccg gtcgtcaacg
     7321 tcagaggttt cacattcgac tgaacatcac tcatgcaccc actctccaga cgcggccccc
     7381 ccgtcgtcgc gccacacgcg cgcggccatt tgcgcctgcc acagcgcctg ataacgtcca
     7441 tgatgtgaga gcaattgcgc atgagtaccc tgctcaacca cctgtccctc ttccatcacc
     7501 agaatgtttc cggccccggc gatggtggat aaacggtgag cgatgataat caccgtccgg
     7561 ttgtgaacca ggttatcgat cgctttttgc accgccagct cgctttcaat atccagcgcg
     7621 gcagtcggtt catcgagaat gacgaccggc gcgtttttca ataacgctct ggcaatggaa
     7681 atccgctggc gctcgccgcc cgatagctgg ccgcccatct ctcccattgg cgtcagccag
     7741 ccttgcggaa gacgggaaat aaactcaagg cactgcgccg cgcgggcggc ctcttctacc
     7801 tcctgccgcg tcgcctgtgg gcgcgcgata cggatattcg ccagcagcgt gtcatcaaac
     7861 agccagacat cctggaaaac aacagaaatt aggctattga gctgttccgg cgtcaggcgg
     7921 cgaatatcga cgccgccaat agaaatctgc ccttgctgcg gatcggcata gcgcattaac
     7981 agtttggtga cggtagtttt gcctgcgccg gaggcaccca ccagcgcgct catactggct
     8041 gccgggaacg tcaaagaaac atgattaagc gcgtggccgt cgccttcttc gtagcgatag
     8101 ctgacgttgt caaagcggat atcgtaacgt tccggcatct cgctttgctc tgcgacggga
     8161 agcggtgcta tcgccataaa ctgctcaatc cgttgcaggg cgctggcgat cagttccaca
     8221 accgaggtgt agctgataaa catcgccatc ggttctgcga agcgcataat catcgcgacg
     8281 gcggcaatca aaaaggcgag gttcagagtg cccgtcacta cccagacgat cccggataac
     8341 accaccacct gtaggcccaa ctccacgacg ctggcgatca gcatcgtcgc gccagcgccc
     8401 tgacggtgag tgcgggtctg taagttttcc agcgcattga aatgcgccag cagcgcccgg
     8461 cttttatcgg catcgctgcc gcaggtgcgc aataccatca tcccctgagc aaattcaacg
     8521 atatcgccgc tcagacgctg gtgcgcttcc cccagcgtct gcatttgtcg ccgcatcgcc
     8581 gggcggcgcc agtaataaaa cggcaccagc agagggaaga tcagcaacat caccagcccc
     8641 agccgccagt ctatccacaa tgtcgccagc gacgccgtca gcggcgtgac aatggtgagc
     8701 agcaaaatat tggctatcgc aataacataa ttgaggtttt catccacgct gcccagcagc
     8761 aaggcgttca tttcacccgc cctgccgcgc tggagcttct ccagcggcac gcggcgtaac
     8821 tgctcgccaa gtcgcaggcg caactcatgg gtagcctgcg ccagatgacc acggtattca
     8881 aagcccaggc cataccagcg tagcaccagc gtcacaattg cggcgacgct gaaggccata
     8941 gcccagttaa gaagttgcgg cgcgtctccc cgtaacagcg catcaatgat cggataaaga
     9001 caggcaaagg cgatcccctg catgaatgcc gccagcaaca gcgccagcat actgcgtcgc
     9061 agcatcctgg cctgactgcc aacgctggag ataagctggc gccagatgac gcgccaggcc
     9121 aggttatccg caggattatt gtctttcatt ttccaccacc tcttcctgtg ttccacccgg
     9181 cacccaatgc tgcgcctgct gatagtgttg ccagagccgt tgatacaggc cgccctgcgc
     9241 caacagttgc gtatggttcc ccatttccct gagctgtccg tcggaaaaca gcaatatcac
     9301 atcggcctga gtcaccattg agagacgatg cgcgaccatg atgaccgtcc ggccacgcat
     9361 ggccgccgcg agcgccttga taagcgccgc ttcgttttcc gggtcagcaa acgccgtcgc
     9421 ctcatcgagc accaggatgg ggcgatcctg caaaagcgcc cgggcgatag taatgcgctg
     9481 ccgctggccg ccggagagaa ataccccacg ttccccgact cgagtgttgt aaccttctgg
     9541 cagagcgcta ataaaatcat gggcctgcgc cactctggcc gccgctataa ccgcctccag
     9601 cggcgtatcc ggcgcgccca gacgaatgtt attggctatc gtgtcggcaa aaaggaagtt
     9661 gtcctgaaac acaaacgaga gttgcttcat cagggtgtcc gtctgcatat cacgcagatc
     9721 cacgccgcca atacggatat ggcctttgtc cgggtcggcg taacgcagca gcaagcgcgc
     9781 cacggtgctt tttccggcgc cgcttggccc gaccagcgcc acaatttgcc cggcaggcac
     9841 atgaaagctc acctcctgca acgcggcgcc agtacgcgct tgcggataat gaaagctcac
     9901 ctgctcaaag gtaatgctcg cctcttgcgg ctgctggtca gactgcggca gcgataactc
     9961 cggcatcgcg agaacctgat aaatacgctg aatgcttaaa cgcgtttgcg cgaccaggtt
    10021 attgagcatc atcattggca ttacggcttc ggccattccg ctgcccagca gtaaaacggc
    10081 cacccacgcg ataaaatcga aactgccata gtgcaacagg ccgtatcccg accagatcag
    10141 gacaaacagg gtcgggagag gattcagaat cgagaaggaa aaacgcgctg aaaaaccggc
    10201 tttgcgatac caggttttga gcacatcgac ccactcttca agggcgcgtt gatagcgcaa
    10261 aaaactggtg ctgccgctat caaacgttct caccacgggc atggcctgca cgaactcaat
    10321 aaccgccgcg ctaacctgct cgcgcgcctt atgataacgc tgagccatat cttccgaacg
    10381 ctggcgggcg agcacgagaa caaccgatcc aaacgccagt acccccagcg tcgcgattgc
    10441 cagccgccag tccagccaga acaagataac gattgtcgcc agcggcatga tgatcgcgcg
    10501 ggcatagagc ggcgtgctgt cggccacaaa aatatgtaac gacttcacgt catccagcat
    10561 cactttcgcc actgacccgc tgcccatttg ctgtaacacg ccaggcggaa gctgcaatgc
    10621 tttacgggct aacctgctgc gcagaatgtt ctccagatga aaagccgcgt aatgagaggt
    10681 gttgaacgcc tgtaagcgca gaacaaaagt taagaccacg cagccgatca cgccgcccag
    10741 cggccaggcc ggaatagcgt caggcgtcgc gcgaatgtca cgcaggctcc acgccagaaa
    10801 aaggagcgcc cccaggctgg ttaatgccgc cagccccgcc agcgccatag ctaaaataac
    10861 cttcctgcga accggtgtaa tcacctgcca gagaggaaat cgggacagcg attcagtatt
    10921 ggatgattga gatgacatgg gagtaactga atttcctgat gaatttttac ggacgccaaa
    10981 aacgacaatt cagggtggcg ttctgagaat taatgtgaat aataaccatt atcaataact
    11041 catctacccc attcggttct atttataccc gcattggtct aagccacagg gagataacca
    11101 ggtcatgatg gagtcaccgc aaacgcaatc tgaaatctct attcaccagt tggtggtcgg
    11161 gaaacctgca aacgatggca atattcctgc tcagtgtgaa ctgctgcgct gttcgttgca
    11221 ggaaggtatg gatattttgc tctggagggg ccattttgcg cggcctgaga cgttgcagct
    11281 acacgacgat ctgggcagga ttaacttctc gtgcatcctg gaaggcacat cgcgctttgc
    11341 gattcaggga ctccggcgcc acaccgactg ggagcttgcg cgcaatcgcc attacattac
    11401 ccatacgcca gactgtcgcg gcagcgcctc ctattgtggc cgattcgaga gcattaccct
    11461 ctccttcagc cctgaaaccc tcgcgctgtg ggtgccggat attagcgcgg tcattaagaa
    11521 taagatcgac tcccactgct gttgccagca gcagcgttgt aatgctgaaa cccacttaac
    11581 ggctcaggcc ttgcgtcacg cgctgatgcg catgcacggc ggcttcagtc atgaacaaaa
    11641 gccgtcgacg ctatggttgc aggggcaaag tctggtcatg ctcagtctgg tgctggatga
    11701 acatcgcgaa gacgccagtt gcctctcctg ccacttcaac ccgatggaac gccagaaact
    11761 gctgcgggcg aaggatctgc tgctggctga tctgacgcaa gcgccgggcg tcgcggagtt
    11821 ggccagagag tccggtctga gcgttctcaa aattaagcga ggcttccgcg ttctgttcaa
    11881 caacagcgtc tatggcctgt ttcaggctga acgaatgcag gaagccagaa gacgcctggc
    11941 caacggcaat acctccgtca tgacggtggc ggccgatctg ggctatgcca acgccagcca
    12001 tttctctgcc gccttccaga aacagtttgg cgtaactcct tcgaccttta aacgcgggat
    12061 gtaaccgggg tcgcgccccc ctaaactcct ccctgacaga ggcccggata ccccatccgg
    12121 gcctctgttc tttccgcctg ttaccaaccc gaaacgggca taaataaacc cgtttcgggt
    12181 agcatccaat tgttaataat tattattctc atatgagtaa tgcttttcgg taagacgtgc
    12241 catcaggagg aagaatgatt tctggcgcac catctcagga ttcgctgtta ccggacaacc
    12301 gccacgcggc tgattaccaa caattacgcg agcggctcat acaggaactg aatttaacgc
    12361 cgcagcagtt acatgaagag agcaacctga tccaggccgg cctggattcc ataagattga
    12421 tgagatggtt acactggttt cgtaaaaatg gctaccgcct tacccttcgc gagctgtatg
    12481 ccgcccccac gctggcggca tggaaccagt taatgctcag ccggtcgccg gagaacgcgg
    12541 aagaagaaac gccgcccgac gaatcatcct ggccgaacat gaccgaaagt acccccttcc
    12601 cattgacgcc agtacagcac gcctacctga cgggccgcat gccggggcag acgcttggcg
    12661 gcgtgggttg ccacctgtat caggagtttg aaggccattg tctgacggcg tcgcagctgg
    12721 agcaggccat cacgaccttg ctgcaacgcc acccaatgct gcatatcgcc tttcgccccg
    12781 acgggcagca ggtctggcta ccgcaacctt actggaacgg cgtcaccgtt catgatttac
    12841 gccataacga cgctgaaagc cgccaggcct atctggacgc actgcgccag cgcctgagcc
    12901 accgtctttt acgcgtggaa atcggcgaaa cgtttgattt tcagctgacg ctcttgccgg
    12961 acaatcgcca ccgcctccat gtcaatattg acctgctgat tatggatgcc tccagcttta
    13021 cgcttttatt cgatgagctt aacgccctgc tggccggaga atcgctgccg gctatcgaca
    13081 cccgctatga tttccgctcg tatttgctgc accagcagaa gatcaatcaa ccactgagag
    13141 acgacgcgcg cgcttactgg ctggcgaaag catcgacgct tccccccgcg cccgtcttgc
    13201 cgctggcctg cgaacccgcc acgctatgtg aagtccgtaa tacccgacgc cgcatgattg
    13261 tcccggcaac acgctggcac gactttagca accgggccgg cgagtatggc gtgacgccga
    13321 caatggcact ggcgacctgt ttttctgccg tgctggctcg ctggggcggc ctgacgcgtc
    13381 tgctgcttaa catcacctta ttcgaccgcc agccgctgca cccggcggtt ggcgcgatgc
    13441 ttgctgactt caccaatatt cttctgctgg ataccgcctg cgatggcgat accgtcagca
    13501 acctggcgcg taaaaaccag ctcacgttta cggaggactg ggagcatcgc cactggtccg
    13561 gcgtcgaatt actccgtgaa ctcaaacgcc agcagcgcta cccccacggc gccccggtgg
    13621 tatttaccag caatctgggg cgttccctct acagcagccg cgcagaatcg ccgttgggcg
    13681 agccggaatg gggcatctcg caaacgccgc aggtctggat agatcatctg gcgttcgagc
    13741 atcacggcga ggtctggcta caatgggaca gcaacgacgc gctgttccct ccggcgttag
    13801 tcgaaacatt gttcgacgcc tactgccagt tgattaacca actctgcgat gacgaaagcg
    13861 cctggcaaaa gccgttcgca gatatgatgc ccgccagcca gcgcgcgata cgcgaacggg
    13921 tcaacgccac cggcgccccc attcccgaag gcttgctgca tgaaggcatt ttccgtatcg
    13981 ctctgcaaca gccgcaggcg ctggcggtaa cggacatgcg ttatcagtgg aattatcatg
    14041 agctgacaga ctatgcccgc cgttgcgcgg gcaggttaat cgagtgcggg gttcagcccg
    14101 gcgataatgt ggctatcacg atgtcgaaag gcgcaggaca acttgttgcg gttctggccg
    14161 tcctgctggc cggggcggtt tacgttccgg tttcgctgga tcagcctgcc gcacggcgcg
    14221 agaaaatcta cgctgacgcc agcgtccggc tggtgctcat ttgtcagcac gacgccagcg
    14281 ccgggtcaga cgatattccc gtccttgcct ggcagcaggc cattgaggcg gagccgatcg
    14341 ccaacccggt agtacgcgcc cccacgcaat cggcctacat tatctacacc tccggctcta
    14401 ccggtacgcc gaaaggggta gtcatttctc accggggagc gcttaacacc tgttgcgata
    14461 tcaatacccg ctatcaggtt ggcccgcatg acagggtgct ggccctctcc gccctacatt
    14521 ttgatttatc ggtttacgac atttttggcg tactgcgcgc gggcggcgcg ctggtgatgg
    14581 tgatggaaaa tcaacggcgc gatcctcacg catggtgtga gctgatccag cgccatcagg
    14641 tcacgctctg gaacagcgtc ccggcgctgt tcgatatgct gctgacctgg tgtgaaggtt
    14701 tcgccgacgc cacgccggaa aacctgcgcg cagtgatgct ttccggcgac tggatcgggc
    14761 ttgacctccc cgcccgttat cgggccttcc ggccacaagg acaatttatc gcgatgggcg
    14821 gtgccaccga ggcgtctatc tggtctaacg cctgcgaaat tcacgacgtc cccgcccact
    14881 ggcgctccat cccttacggt tttccgctaa ccaaccaacg ctaccgggtg gtggatgaac
    14941 agggccggga ctgccctgac tgggtgccgg gtgaattatg gattggcggc attggggtcg
    15001 cggaaggcta tttcaacgat cccctgcgta gcgagcagca atttttgacg ctcccggacg
    15061 agcgctggta tcgcaccggc gatctcggct gctactggcc agatggcaca atcgagttcc
    15121 tcggtcgtcg cgacaagcag gtcaaagtcg gaggatatcg catcgagctg ggcgaaatcg
    15181 aaagcgcgct cagccagctg gcgggggtga aacaagcaac cgttctggcg atcggcgaaa
    15241 aagaaaaaac gctggcggca tacgttgttc ctcagggcga ggctttttgc gttaccgatc
    15301 atcggaaccc ggcactgccg caggcgtggc acacgcttgc gggaacgttg ccctgttgcg
    15361 ccatctcgcc agagatctcc gcagaacagg tagccgattt ccttcagcat cgcctgctaa
    15421 aactgaagcc gggtcacacc gctggcgccg atcctctccc cctgatgaac tcactcgcta
    15481 tccagccgcg ctggcaggcc gtggtggaac gctggttagc atttctggtg acacaacggc
    15541 gactgaagcc cgctgctgaa ggttatcagg tctgcgctgg tgaagaacgc gaggatgagc
    15601 acccgcactt cagcggacat gatttaacgt tatcgcaaat tcttcgcggt gcccgtaacg
    15661 aactgtcgtt actgaacgac gcgcagtggt ccccggaaag cctggccttt aaccatccgg
    15721 ccagcgcccc gtatattcag gaactggcga caatttgcca acagcttgca cagcgcttac
    15781 agcgcccggt acgcctgctt gaggtgggaa cccgcactgg ccgcgccgca gaatcgctgt
    15841 tagcacagct caacgccgga cagattgagt atgtcgggct tgagcagagc caggagatgc
    15901 tgctgagcgc ccggcagagg ctcgccccct ggcctggcgc ccgtctgtcc ctctggaatg
    15961 cagacacgct ggcggcgcac gctcactcgg cggacattat ctggcttaat aacgccctgc
    16021 atcgtctgct gccggaagat cccgggctcc ttgcgacatt acaacagctt gccgttcccg
    16081 gcgcgctgct ctacgtgatg gagtttcgcc agttaacgcc gtccgcccta ctcagcacgc
    16141 tcctgttaac caatgggcag ccggaggcct tgctgcataa cagcgccgac tgggcggcat
    16201 tatttagcgc ggccggcttc aactgtcagc atggcgatga ggtcgcgggg ttacaacgct
    16261 tcctcgtaca atgtcctgac aggcaggtgc gccgcgatcc ccgtcaactt caggccgccc
    16321 tcgccgggcg tctgccgggg tggatggtgc cgcaacggat cgtcttcctc gacgccttac
    16381 cgctgacggc taacgggaaa attgactacc aggcgctgaa gcgtcgtcat acccctgaag
    16441 cggaaaaccc ggccgaagcg gatttacccc agggcgacat tgaaaaacag gttgccgccc
    16501 tctggcagca actcttatca actggcaatg tcaccagaga aaccgacttc ttccagcaag
    16561 gcggcgatag cctgctggcg acccgtctga ccgggcaact tcatcaggca ggttatgaag
    16621 cgcaattaag cgacctgttt aatcatcccc ggctggcgga ttttgccgcc acgctgcgga
    16681 aaaccgacgt cccggtcgaa caaccattcg tccactcccc tgaagatcgc taccagccct
    16741 ttgcgcttac cgacgtgcag caggcttacc tggtggggcg tcagccgggc tttgccctgg
    16801 gcggcgtcgg ctcacatttc tttgttgaat ttgaaattgc cgatctggat ctcacccggc
    16861 tggagacggt ctggaaccga ttaatcgccc gccacgatat gctacgcgcc gtcgtgcgtg
    16921 atggacagca acaggtgctc gaacagacgc ctcgctgggt aatacccgca cacatcctcc
    16981 atacgcctga agaggcgttg caggtgcgcg aaaaactggc ccatcaggta ctcaaccccg
    17041 aagtctggcc ggtattcgat ctccaggtcg gttacgttga cgggatgccc gcccgcctgt
    17101 ggctgtgtct ggataacctg ttacttgacg ggctgagcat gcagattctg ttggcggagc
    17161 tggaacatgg ctaccaatat ccgcaacagt tgcctccgcc gctacccgtc acctacaggg
    17221 attacctgca acaacccgcg atccagtcgc ttaacgcaga ttctctggca tggtggcagg
    17281 cgcaacttga tgatattcct ccggcgcctg cgttgccgct gcgctgcttg cctcaggagg
    17341 ttgaaacacc gcgcttcgcc cgcctgaacg gcgcgctgga cagcacgcgc tggcatcggc
    17401 tgaaaaaacg ggcggctgac gcccatctca ccccgtcggc cgtactgttg tcggtgtggt
    17461 caacggttct ctctgcatgg agtgcacagc ctgagttcac gcttaacctt acgcttttcg
    17521 acaggcgacc gctgcacccg caaatcaacc agattctggg cgatttcacc tcgctgatgc
    17581 tgctgagctg gcatcccggc gaaagctggc tgcacagcgc gcagtcacta cagcagcggc
    17641 tgagccagaa cctcaaccac cgcgatgtgt cagccatccg cgtgatgcgt caactggcgc
    17701 aacggcaaaa cgtgcctgcc gttccgatgc ccgtcgtctt taccagcgca ctgggctttg
    17761 agcaggataa cttcctcgcc cggcgtaatc tgctcaaacc ggtctggggc atctcccaga
    17821 cgccgcaggt ctggctcgat caccagattt atgaatccga aggcgaactg cgctttaact
    17881 gggattttgt cgccgcgctg tttcctgccg ggcaggtgga gcgccagttt gaacagtatt
    17941 gcgcattgct aaaccgaatg gccgaggatg aaagcggctg gcaactgccg ctcgccgcgc
    18001 tggtgcctcc cgttaaacac gcagggcaat gcgcagagcg ctcaccgcgc gtatgccctg
    18061 agcactctca gccacacatt gcggcggacg agagcaccgt cagcctgatt tgcgacgcct
    18121 tccgcgaggt ggttggcgag tctgtcacgc ccgcagaaaa cttctttgag gcgggcgcaa
    18181 cgtcgctgaa tctggtgcaa ctgcacgttt tgttacaacg tcacgaattt tccaccctga
    18241 cgttgcttga cctcttcacc cacccttctc ctgctgccct ggccgattat ctggccggcg
    18301 tcgccacggt ggagaaaaca aaacgacctc gccctgttcg ccgtcgtcag cggcggatat
    18361 agcgcgaagc aaactgattt tccccggaac gccatcgcga acgcatggcg ttccattgac
    18421 tttgtgaatc ttaggaaacg ggaccgatta tggataactt gcgcttctct tctgcgccga
    18481 cagcagattc cattgatgca tcgatcgctc aacactaccc ggactgcgaa cctgtcgcgg
    18541 ttatcggcta cgcctgccat tttcctgaat cgccggatgg cgaaacgttc tggcaaaatc
    18601 tgctggaagg tcgtgaatgc agccgacgct ttacgcgcga agagcttctg gccgtcggtc
    18661 tggatgccgc catcattgac gatcctcatt atgtcaatat cggtacggtg ttagacaacg
    18721 ccgactgctt cgatgccgct ctgtttggct attctcgaca ggaagcggag tcaatggacc
    18781 cgcagcagcg tctgtttttg caggcggtct ggcatgcgct ggaacatgcc ggttatgccc
    18841 ccggcgccgt cccccataag accggcgttt tcgcctcttc ccggatgagt acctaccccg
    18901 gtcgcgaagc attgaacgtg acagaagtcg cgcaggtaaa aggtctgcaa tctctgatgg
    18961 gcaatgataa agactatatt gccacccgcg ccgcgtacaa actcaacctg cacggcccgg
    19021 cgttatcggt acagaccgcc tgctccagct cgctggttgc cgtgcatctg gcctgtgaaa
    19081 gcctgcgcgc aggcgaatcc gatatggcgg ttgccggcgg cgtggcgctc tctttccccc
    19141 agcaggcagg ctaccgctac cagcccggaa tgattttctc tcctgatggt cactgtcgtc
    19201 cctttgacgc ctcggctgag ggcacctggg ccggtaacgg tctcggctgc gtggtgctgc
    19261 gtcgcctgag agacgcgctg ctgtcaggcg atccgattat ctcggtgatc ctctccagcg
    19321 cggtcaacaa cgacggcaac agaaaggtcg gctataccgc cccttccgtc gcagggcaac
    19381 aagcagtcat cgaagaggcg ttaatgctgg cggccatcga cgacaggcag gtaggttaca
    19441 ttgaaaccca cggcaccggc acaccgctgg gcgacgcgat tgaaattgaa gcgttacgca
    19501 acgtctatgc gcctcgcccg caggacaggc gctgtgcgct cggttccgtg aaaagtaaca
    19561 tgggccatct ggataccgcg gcgggcattg ccggactgct gaaaaccgtt ctggcagtca
    19621 gtcgcgggca aattcctccc ttactgaatt ttcacacccc caacccggca ctgaaacttg
    19681 aagagagccc ctttaccata ccggtgtcgg cacaggcatg gcaggacgaa atgcgctatg
    19741 cgggcgtctc ctcctttggt attggcggca ccaactgcca tatgatcgtc gcctcgctgc
    19801 ccgacgcgct caacgcgcgc ctccccaata cggatagcgg cagaaaaagt accgcgctgc
    19861 tgctcagcgc cgccagcgac agcgcgttgc ggcggctggc gacggattat gccggggcgc
    19921 tgagagagaa tgcggatgcc agctctctgg ccttcacagc cctgcacgcg cgccgtctcg
    19981 atctcccctt ccgcctggcg gcgccattaa accgtgaaac cgccgaggcg ctcagcgcct
    20041 gggccggtga gaaatcgggg gcgctggttt acagcggcca cggcgccagc ggcaagcagg
    20101 tgtggctgtt taccggccag ggctcgcact ggcgcactat gggtcaaacg atgtaccagc
    20161 actcaacggc gtttgccgac acgctggatc gctgtttttc cgcctgtagc gaaatgctca
    20221 tgccgtcact gcgcgaagcg atgtttaacc ccgattcggc gcagctggac aatatggcct
    20281 gggcgcagcc ggcgattgtc gcgtttgaaa tcgcgatggc ggcgcactgg cgtgctgaag
    20341 gactgaagcc agacttcgcc attgggcatt ccgtcggtga atttgccgct gccgttgtct
    20401 gcggacacta tacgattgaa caggtcatgc cactggtttg tcggcgcggc gcgctaatgc
    20461 agcagtgcgc aagcggcgcg atggtggcgg tatttgcaga cgaagacacg ctgatgccgc
    20521 tggctcgcca gtttgagctg gatctcgccg ccaacaacgg tacgcaacat acggtatttt
    20581 ccgggccgga agcccgtctc gcggtatttt gcgccacgct ctcgcagcat gacattaact
    20641 atcgtcgcct gagcgtaacc ggtgcggcgc actccgcttt actggagccg atactcgatc
    20701 ggttccagga cgcctgcgcg ggactgcacg cggagccggg gcaaataccg attatttcca
    20761 cgctcaccgc cgacgtcatt gatgagtcaa cgctcaacca ggcggattac tggcgccgac
    20821 acatgcgcca gccggtgcgt tttatccaga gtattcaggt ggcgcatcag ctcggcgccc
    20881 gcgtttttct ggagatgggg cccgatgccc agttggttgc ttgcgggcag cgcgaatacc
    20941 gcgataacgc atactggata gccagcgccc ggcgtaacaa agaggcgagc gatgtcctca
    21001 atcaggccct gctccagctt tacgctgccg gcgtcgccct accgtgggcc gacctgctgg
    21061 cgggcgatgg acaacgtatc gctgcgccat gttatccgtt tgatactgaa cgttactgga
    21121 aagagcgcgt ctccccggcc tgcgagcctg ccgacgcagc gctgtctgcc gggctggagg
    21181 tggcgagtcg cgccgcgaca gcgctcgatc tccctcgcct ggaagcgctt aaacagtgcg
    21241 ccacgcgact gcacgccatc tacgtcgatc aactggtaca acgctgtacc ggcgatgcca
    21301 ttgagaacgg cgtggacgcc atgaccatca tgcgccgtgg acgtctgctg ccccgctacc
    21361 agcagctact ccagcgcctg ctgaataact gcgtggtcga cggcgattac cgctgcaccg
    21421 acgggcgata cgtccgcgcc cgccccattg aacatcaaca gcgggaatca ctgctgacgg
    21481 aacttgccgg ttattgtgaa ggttttcagg ctattcccga caccatcgcc cgtgccggcg
    21541 atcggttata tgaaatgatg agcggcgcgg aagaaccggt ggcgattatc ttcccgcaaa
    21601 gcgcctccga cggcgtggaa gtgctgtatc aggaattcag ctttggccgc tatttcaacc
    21661 aaatcgccgc cggggtatta cgcggcattg tccagacgcg tcagccccgc cagccgttgc
    21721 gtattcttga agttggcggc ggaaccggcg gcaccaccgc gtggctgctg ccggaactca
    21781 acggcgttcc ggcactggag taccatttca ccgatatctc ggcgctgttc acccgtcgcg
    21841 cccagcagaa attcgccgac tacgattttg tgaagtatag cgagctggat ctcgaaaaag
    21901 aggcgcagtc tcagggtttc caggcacagt cttacgatct tatcgtggca gcgaacgtga
    21961 ttcacgccac ccgccatatt ggccgcacgc tcgataatct gcgccccctg ctcaagccgg
    22021 gcgggcgcct gctgatgcgc gaaatcaccc agccaatgcg tctgtttgac ttcgttttcg
    22081 gcccgctggt tcttccgcta caggatctcg acgcccgcga aggtgagtta ttcctcacca
    22141 ccgctcagtg gcaacaacag tgccgccacg ccggattcag caaagtggcg tggctaccgc
    22201 aggatggcag cccgaacgcc gggatgagcg aacatatcat tctcgccacg ctgcccggtc
    22261 aggcggttag cgccgtaaca ttcaccgcgc catcagaacc cgtgttgggg caggcgctga
    22321 cggataacgg tgattatctc gccgactggt ctgattgcgc aggtcagccc gaacagttta
    22381 acgcccgctg gcaggaggca tggcgtctgc tttcacagcg tcatggcgac gctctgcctg
    22441 tggaaccgcc ccccgtcgcc gccccggagt ggctggggaa ggttcgctta agctggcaaa
    22501 acgaagcctt ttcccgcggt cagatgcgcg ttgaagcccg tcatcctgct ggcgagtggc
    22561 tgccgctatc gcccgccgcg cctcttcctg cgccgcaaac gcattatcaa tggcgctgga
    22621 cgcccctcaa cgtcgccagc attgaccatc cgcttacctt tagcttcagc gccggtacgc
    22681 ttgcgcgcag cgacgagctg gcgcaatacg gcatcattca cgatccgcac gcctcttcac
    22741 gactgatgat tgttgaggag agcgaggata cgctggcctt agcggagaaa gtgatagcag
    22801 cgctcaccgc cagcgcagcc ggattgattg tggttactcg ccgcgcgtgg cgagtcgagg
    22861 aaaatgaagc actctctgca tcccatcacg cgctatgggc cttgcttcgc gtcgcggcca
    22921 acgaacagcc ggaacggttg cttgccgcca tcgatctcgc cgaaaacacc ccgtgggaaa
    22981 cgctgcatca agggttgagc gcagtctcac tatcacagcg ctggctcgcc gcacggggtg
    23041 acaccctttg gctcccttca ctggcgccca atacgggatg cgccgctgaa ttaccggcaa
    23101 acgtgtttac cggcgatagc cgctggcatc tggtgaccgg agcgtttggc ggattaggcc
    23161 gccttgccgt gaactggctc agagaaaaag gggcgcgacg catcgccctg ctggcgccgc
    23221 gcgtggatga gtcatggcta cgcgacgtgg agggcgggca gacgcgcgtc tgccgttgtg
    23281 atgtgggcga tgccgggcaa ctggccacgg ttcttgacga tctggcggcc aacggcggca
    23341 ttgccggagc gattcatgcc gctggcgtat tggctgacgc gcccttgcag gagcttgatg
    23401 accaccagct ggctgccgtt ttcgcggtaa aagcgctggc ggcaagccag ctgttgcaaa
    23461 ccctgcgcaa ccacgacgga cgctatctta ttctctactc ttccgctgcc gccaccctcg
    23521 gcgcgccggg tcagagcgcc catgcgctgg cctgcggcta cctggacggg ctggcccagc
    23581 agttttccac ccttgatgcg ccgaaaacgc tctctgtcgc ctggggcgca tggggagaaa
    23641 gcggtcgggc ggccacgccg gaaatgctgg cgacgctcgc cagccgaggt atgggcgcgt
    23701 taagcgatgc cgaaggctgc tggcacctgg aacaggcggt gatgcgcggt gccccgtggc
    23761 gactggcgat gcgcgttttt accgacaaaa tgcccccgtt acaacaggct ctgtttaaca
    23821 tcagcgccac agaaaaagcc gcaacgccgg tcattcctcc tgctgatgac aacgccttta
    23881 acggcagcct gagcgatgaa acagcggtga tggcatggct gaaaaagcgg attgcggttc
    23941 agctaaggct gagcgatccg gcgtcactgc atccaaacca ggatctgttg caactcggca
    24001 tggactcgct gctcttcctt gaactcagta gcgatattca gcactacctg ggcgtacgca
    24061 tcaatgcgga gcgggcgtgg caggatctgt ctcctcatgg actcacgcag cttatctgtt
    24121 ctaagccaga ggcgacgcct gccgcttcgc agccggaagt gttgcggcac gacgccgacg
    24181 agcgttatgc gcccttccct ttgacgccca ttcagcacgc ctactggctg gggcgaaccc
    24241 acctcattgg ctatggcggc gtcgcctgtc acgtcctgtt tgagtgggat aaacgccacg
    24301 atgagttcga tctcgccata ctggagaaag catggaacca gctcatcgca cgccacgata
    24361 tgttgcgtat ggtggttgat gccgacgggc agcagcgaat cctggcgaca acgccggagt
    24421 atcacatccc gcgtgacgat ctgcgcgcgc tttccccgga agaacagcgc atcgcgctgg
    24481 aaaaacggcg gcatgaactg agctatcgcg ttttgcctgc cgaccagtgg cctctttttg
    24541 agctggtggt cagcgaaatc gacgattgcc attaccgtct gcatatgaac ctcgaccttt
    24601 tgcagtttga tgtgcagagt tttaaagtca tgatggacga cctggcgcag gtctggcgcg
    24661 gtgaaacgct ggcgccgctc gctattacct tccgtgatta tgtgatggct gaacaggcgc
    24721 gccgacagac atcggcatgg cacgatgcct gggattactg gcaggaaaaa ctgccgcaac
    24781 tgcccttagc gccagagctg ccggtggttg agacgccccc ggaaacgcca cacttcacca
    24841 ccttcaaatc gacgatcggc aagacagaat ggcaggccgt gaaacagcgc tggcagcagc
    24901 aaggcgtcac accgtctgcc gcgctgctca cgctgtttgc cgccaccctt gagcgctgga
    24961 gccgtaccac aacatttacg ctgaacctga cgttcttcaa tcgccagccg atccatccgc
    25021 aaatcaacca gttgattggt gattttacct ccgtcacgct ggttgatttt aacttctcag
    25081 cgccggtgac gttgcaagag cagatgcaac agacccaaca gcgcctctgg caaaacatgg
    25141 cgcacagtga aatgaacggt gttgaggtga tccgtgagct gggccgcctg cgcggatcac
    25201 aacgtcaacc gctgatgccg gtagtgttta ccagtatgct ggggatgacg ctggaaggca
    25261 tgactatcga tcaggcgatg agccatctgt tcggcgaacc ctgctatgta ttcacgcaaa
    25321 cgccgcaggt ctggctggat catcaggtca tggagagcga cggcgagttg atgtttagct
    25381 ggtactgcat ggacaacgtg ctggaacccg gcgctgccga ggcgatgttt aatgactatt
    25441 gcgccatcct gcaagccgtc atcgccgccc ctgaaagcct gaagactctc gccagcggca
    25501 tcgccaggca cattccccgc cgacgctggc cgctgaacgc gcaggcggac tacgacctgc
    25561 gggatattga gcaggcgacg ctcgaatacc ccggcatccg gcaggccaga gcggaaataa
    25621 ccgaacaggg cgcgttgacg ctggatatcg tgatggccga cgatccgtcg ccatcagcgg
    25681 cgatgcctga tgagcacgaa cttacccaac tggcgctgcc gttgcctgag caggcgcagc
    25741 ttgatgagct ggaggcgacc tggcgctggc tggaggcgcg agcgctacag gggatcgcgg
    25801 ctacgctaaa tcgtcacggc ctgtttacca cgccggagat cgcccatcgc tttagcgcaa
    25861 tagtacaggc gctgtccgcg caagcgtctc accagcgtct gctgcgccag tggctacagt
    25921 gtctgacgga aagagagtgg ttaatccgcg aaggtgaaag ctggcgctgc cgcattccgc
    25981 tcagcgagat tcctgagcct caggaagcgt gcccgcaaag ccaatggagc caggcgctgg
    26041 cgcagtatct ggaaacctgc atcgcccggc acgacgccct cttctccggg cagtgttctc
    26101 cgctggaatt gctgttcaac gagcagcatc gcgttaccga cgcgctgtat cgcgacaacc
    26161 ccgccagcgc ctgtctgaat cgctataccg cgcagattgc cgccttgtgc agcgcagaac
    26221 ggattctgga ggttggcgcc ggaaccgcag ccactaccgc gccggtgctg aaggccacgc
    26281 ggaacacgcg gcagtcgtac cacttcacgg acgtctccgc gcagttcctc aatgacgcca
    26341 gagcccgttt ccatgatgaa tcgcaggtgt cttatgcctt gttcgacatc aaccagccgc
    26401 tggatttcac cgcccacccg gaggcgggtt acgacctgat cgttgccgtc aatgtgctcc
    26461 acgacgccag ccatgtcgtc cagacgttgc gcagattaaa actgttgctg aaagccggcg
    26521 gacgtttgct gatcgttgaa gcgacggagc gaaacagcgt attccagctg gcgagcgtgg
    26581 gctttattga gggattaagc ggataccgcg atttccgccg ccgggatgag aaaccgatgc
    26641 tcacccgctc cgcatggcag gaggttctcg ttcaggccgg gtttgcaaac gagctggcgt
    26701 ggcccgcgca ggaatcgtcg ccgctgcgcc agcatctgct ggtagcacgt tcgcctggcg
    26761 taaatcgccc ggataaaaaa gccgtgagcc gctatttaca gcagcgcttt ggcaccggtc
    26821 tgcccatttt acagatccgg caaagagaag cgttatttac gccgctgcat gccccgtctg
    26881 atgcgccgac tgagccagcc aaacccacgc cagttgccgg ggggaatccg gcactggaaa
    26941 aacaggtggc tgaactctgg caatcgctgc tgtctcgccc cgtggcaagg catcacgact
    27001 ttttcgaact gggcggcgac agcctgatgg cgacaaggat ggtcgcgcag ctaaaccgga
    27061 gagggattgc cagggctaac cttcaggatc tgttcagcca ttcgacgctg agcgacttct
    27121 gcgcccatct acaggcggct acgtcaggag aggacaaccc gatacccctt tgccagggcg
    27181 acagtgagga aaccctgttt gtcttccacg cttcagacgg cgatatcagc gcctggctgc
    27241 cgctcgctag cgcgttgaac aggcgcgttt tcggcctgca agcaaaatcg ccgcagcgct
    27301 ttgccacgct cgaccagatg atcgatgagt atgtcgggtg catccgtcgt cagcagcctc
    27361 acggccctta tgtgctggcg ggttggtcgt atggcgcgtt tcttgcggcg ggcgccgcac
    27421 aacgcctgta cgccaaaggc gagcaggttc ggatggtgtt aatcgatccc gtgtgccgac
    27481 aggatttctg ttgcgaaaac cgggcggccc tgctgcgcct gttagccgaa ggacaaacgc
    27541 ctctggcact gcccgaacat ttcgaccagc agacgcccga cagccagctt gccgacttta
    27601 tcagcctcgc taaaacggcc ggtatggtgt cgcaaaacct gacgctgcaa gcggcagaaa
    27661 cgtggctcga caacatcgcg catctgctgc gtttactgac tgagcatacg ccgggcgaaa
    27721 gcgttccggt cccctgtctc atggtgtatg ccgccgggag acccgcgcgc tggacgccag
    27781 cagaaaccga gtggcagggc tggataaaca acgccgacga cgctgtgatt gaagccagcc
    27841 actggcaaat catgatggaa gccccccacg ttcaggcttg tgcgcaacac attacgcgct
    27901 ggctttgcgc aacctcaacg caaccggaga acacgttatg atgccgtccg cctccccaaa
    27961 acaacgcgta ctgattgtgg gcgccaaatt tggcgaaatg tacctgaatg cctttatgca
    28021 gcccccggag gggctggaac tggtcggcct gctggcgcag ggaagcgccc gttcaagaga
    28081 gctggctcat gcgtttggca ttccgctgta tacctcgccg gaacagataa ccaggatgcc
    28141 ggatatcgcc tgtattgtcg tgcgttcgac cgtcgccgga ggaaccggta cgcagcttgc
    28201 cagacacttt ctgacgcgcg gcgtacacgt cattcaggag catcccctcc acccggatga
    28261 catcagttcc ttacaaacgc tggcgcagga acagggctgc tgctattggg taaacacgtt
    28321 ttatccgcat acgcgcgcgg gacgcacatg gcttcgcgat gcgcagcagc tgcgtcgctg
    28381 cctggctaag acgccgccgg tagtccacgc caccaccagc cgacagttgc tctactccac
    28441 tctggatttg ttgttgctgg ctctgggcgt tgatgccgcc gccgtggagt gcgacgtggt
    28501 tggcagcttt agcgattttc actgtctgag actcttctgg ccggagggag aagcctgcct
    28561 gctgcttcag cgctatctcg atcctgacga tccagatatg catagcctta tcatgcaccg
    28621 cctgctgctg ggctggccgg aaggtcatct ttcgctggag gcgagctacg gccccgtcat
    28681 ctggtcatcc agcctgtttg tcgcagacca tcaggagaac gcccacagcc tctaccgcag
    28741 accggagatc ctgcgcgatc tgccgggtct gacgcgcagc gcggcgcccc tgagctggcg
    28801 cgactgctgc gaaaccgtcg ggccggaggg cgtcagctgg ttgctacacc agttgcgcag
    28861 ccatctggcg ggcgaacatc caccagcggc ctgccaaagc gtacatcaga tcgcgttatc
    28921 acgtttatgg cagcagattt tacgtaagac cggcaatgcg gagatcagac gcctgacgcc
    28981 gccgcaccac gaccggttag ccggtttcta caacgatgat gataaggagg cgctgtgacg
    29041 caatctgcaa tgtgcatccc gctgtggccc gcccggaacg gcaatactgc gcatctggtc
    29101 atgtgccctt tcgctggcgg cagcagtagc gcgtttcgcc actggcaagc tgagcaactg
    29161 gctgattgcg cgctttctct ggtgacctgg ccggggcgcg atcgccttcg ccatctggaa
    29221 ccgctcagaa gcattacaca actggcggca ctgctggcga acgagctgga agcatccgta
    29281 tcgcctgaca cgccgctttt actcgccggg cacagcatgg gggcgcaggt ggcgtttgaa
    29341 acctgccgac ttctggagca acgggggctt gcgccacaag gactgattat ttccgggtgc
    29401 catgccccgc atctgcattc tgaacgccag ctcagccatc gcgatgatgc cgactttatc
    29461 gctgagctga tagacattgg cggatgttct cctgaactgc gggaaaacca ggaattaatg
    29521 tcgctgtttc ttcctcttct gcgcgctgat ttttacgcca ccgagagcta tcactacgac
    29581 tcgcccgacg tctgtccgcc gctgcgcacg cctgcgctgt tattgtgcgg cagccacgat
    29641 cgcgaagcct cctggcagca ggtcgatgcc tggcgtcagt ggctgagcca cgttacaggc
    29701 ccggtggtga ttgacggcga tcatttctat cccattcaac aagcccggtc cttttttacg
    29761 cagattgtcc gccattttcc ccacgcattt tctgcaatga ccgcgtggca aaaacagccc
    29821 agtacttcag aaaggtgacg catgaattct tcctttgaat ctctgattga acagtatccc
    29881 ttacccattg ccgaacagtt gcgccactgg gcggcccgtt atgcctcgcg aattgccgtc
    29941 gttgatgcaa aggggtcgtt aacctacagc gcgcttgatg cacaagttga cgaacttgcc
    30001 gcaggtctgt catcactggg tttgcgttcg ggggagcatg taattgtgca gcttcccaac
    30061 gacaacgcgt ttgttaccct gctgttcgcc ttgttaagac tgggcgttat ccccgtgctg
    30121 gcgatgccct cgcaacgggc gctggatatc gacgcgctga ttgagctggc gcaacccgtc
    30181 gcttacgtta ttcacgggga aaaccacgca gagctggccc gacagatggc gcacaaacac
    30241 gcctgcttgc gtcatgttct ggtcgctgga gagaccgtga gcgacgattt tacgccgctc
    30301 ttctcccttc acggtgagcg acaggcatgg ccgcagcctg atgtttccgc caccgcgttg
    30361 ttgttgctct caggcggcac aaccggcacg cccaaactca tcccgcgccg acatgccgac
    30421 tatagctata acttcagcgc ttctgctgaa ctgtgcggca tcagccaaca gagcgtgtat
    30481 ctcgccgtcc tcccggtggc gcataacttt ccgctggcct gccccggtat tctgggaacg
    30541 cttgcctgcg gcggaaaagt ggtgctgacc gacagcgcca gctgtgatga ggtgatgcct
    30601 ttaatcgcgc aggaaagagt gactcacgtc gccctggttc cggcgctggc gcaattatgg
    30661 gtgcaggcca gggagtggga agacagcgac ctttcgtcgc tgcgcgtcat tcaggcaggc
    30721 ggcgcccggc tcgacccgac gcttgctgag caggttatcg ccacctttga ctgtaccctg
    30781 caacaggttt tcggtatggc ggaaggcctg ctctgtttta cccggctgga cgatccgcat
    30841 gccaccattc tccacagcca ggggcgcccg ttgtcccctc tggatgaaat ccgcatcgtt
    30901 gatcaagacg agaacgacgt cgcgccgggc gaaaccgggc aattgttaac gcgcggccct
    30961 tataccattt cgggctatta ccgcgcccct gcccacaatg cgcaggcctt taccgcgcaa
    31021 gggttttacc gcacaggcga caatgtcagg ctggatgagg tggggaacct gcacgttgag
    31081 ggacgcataa aagagcagat caaccgcgcc ggagaaaaaa tagccgcggc tgaagtggaa
    31141 tcggcactgc tgcgtttagc ggaagtgcaa gattgcgcgg tggtcgccgc gccggacacg
    31201 ctgcttggcg agcggatttg cgcgtttatc atcgcgcagc aggtgccaac tgactatcag
    31261 cagttgcgtc aacaactgac ccgtatgggg ctcagcgcgt ggaaaattcc tgaccaaatc
    31321 gagtttctgg accactggcc gctcaccgcc gtcggcaaga tagacaaaaa acgcctgacg
    31381 gctctcgccg tcgaccgtta tcgccattct gcccaataag cgcaaaccga cccgaaacag
    31441 gttgaaataa acccgtttcg ggtagcacca ctattagaaa tagttatcat tttcaattca
    31501 ccattgtcgg tatttttggc gtttcgccgt cttacaggga ctcacaacaa tgaaaatgac
    31561 acggctttat cctctggcct tggggggatt attgctcccc gccattgcta atgcccagac
    31621 ttcacagcaa gacgaaagca cgctggtggt taccgccagt aaacaatctt cccgctcggc
    31681 atcagccaac aacgtctcat ctactgttgt cagcgcgccg gaattaagcg acgccggcgt
    31741 caccgccagc gacaaactcc ccagagtctt gcccgggctc aatattgaaa atagcggcaa
    31801 catgcttttt tcgacgatct cgctacgcgg cgtctcttca gcgcaggact tctataaccc
    31861 cgccgtcacc ctgtatgtcg atggcgtccc tcagctttcc accaacacca tccaggcgct
    31921 taccgatgtg caaagcgtgg agttgctgcg aggcccacag ggaacgttat atggcaaaag
    31981 cgctcagggc gggatcatca acatcgtcac ccagcagccg gacagcacgc cgcgcggcta
    32041 tattgaaggc ggcgtcagta gccgcgacag ttatcgaagt aagttcaacc tgagcggccc
    32101 cattcaggat ggcctgctgt acggcagcgt caccctgtta cgccaggttg atgacggcga
    32161 catgattaac cccgcgacgg gaagcgatga cttaggcggc acccgcgcca gcatagggaa
    32221 tgtgaaactg cgtctggcgc cggacgatca gccctgggaa atgggctttg ccgcctcacg
    32281 cgaatgtacc cgcgccaccc aggatgccta tgtgggatgg aatgatatta agggccgtaa
    32341 gctgtcgatc agcgatggtt caccagaccc gtacatgcgg cgctgcactg acagccagac
    32401 cctgagtggg aaatacacca ccgatgactg ggttttcaac ctgatcagcg cctggcagca
    32461 gcagcattat tcgcgcacct tcccttccgg ttcgttaatc gtcaatatgc ctcagcgctg
    32521 gaatcaggat gtgcaggagc tgcgcgccgc aaccctgggc gatgcgcgta ccgttgatat
    32581 ggtgtttggg ctgtaccggc agaacacccg cgagaagtta aattcagcct acgacatgcc
    32641 gacaatgcct tatttaagca gtaccggcta taccaccgct gaaacgctgg ccgcatacag
    32701 tgacctgacc tggcatttaa ccgatcgttt tgatatcggc ggcggcgtgc gcttctcgca
    32761 tgataaatcc agtacacaat atcacggcag catgctcggc aacccgtttg gcgaccaggg
    32821 taagagcaat gacgatcagg tgctcgggca gctatccgca ggctatatgc tgaccgatga
    32881 ctggagagtg tatacccgtg tagcccaggg atataaacct tccgggtaca acatcgtgcc
    32941 tactgcgggt cttgatgcca aaccgttcgt cgccgagaaa tccatcaact atgaacttgg
    33001 cacccgctac gaaaccgctg acgtcacgct gcaagccgcg acgttttata cccacaccaa
    33061 agacatgcag ctttactctg gcccggtcgg gatgcagaca ttaagcaatg cgggtaaagc
    33121 cgacgccacc ggcgttgagc ttgaagcgaa gtggcggttt gcgccaggct ggtcatggga
    33181 tatcaatggc aacgtgatcc gttccgaatt caccaatgac agtgagttgt atcacggtaa
    33241 ccgggtgccg ttcgtaccac gttatggcgc gggaagcagc gtgaacggcg tgattgatac
    33301 gcgctatggc gcactgatgc cccgactggc ggttaatctg gtcgggccgc attatttcga
    33361 tggcgacaac cagttgcggc aaggcaccta tgccaccctg gacagcagcc tgggctggca
    33421 ggcgactgaa cggatgaaca tttccgtcta tgtcgataac ctgttcgacc gtcgttaccg
    33481 tacctatggc tacatgaacg gcagcagcgc cgtcgcgcag gtcaatatgg gtcgcaccgt
    33541 cggtatcaat acgcgaattg atttcttctg attattgtaa aagggatacc gaaaaggtat
    33601 cccttttaca ccactagtta aaaccagtaa ctcagcagag tcgcaaaaaa tattaatcca
    33661 tagtgattat ttaaacaatg aaattgcgat taggataaat agatttaact ttctcgttcc
    33721 tttctctcct tatactaaag aaataatcat atcaaaataa aaattcacaa cagtgcaaca
    33781 ttaaaaaata caaccaacaa acaatcctat atacaaggaa catctccaga atataaaagc
    33841 acagacaaac aacctaaaaa aacaacccca tacatataaa ttattttata ggtaaaatag
    33901 attatatttt ctcatagcta ccacaaacta tactagcctg aactatattt attctgctgc
    33961 aatcaatgca tacataacac aaatatcact caggtacact actcaaacca cgctgggatt
    34021 tttcctcaag ttataatcat caccccgaaa atcattcggc atttactcat taaatagtca
    34081 ccccataggc ctgtacatgt ttactcagaa atatacatcc ttttctctgt cataaaccct
    34141 ctgattaatc atagataaat acttgtgaca ccaatctttt tccttaaagg aacgaattgt
    34201 tgtgtagaag gagataatat tatgtcaaaa aaatatcagc cattgcttat aactcattat
    34261 atgtcaacat gggtcactat aacggaagca gttgagatca ctaccaaagc cataaaacaa
    34321 aaaattactc ctagcgatat ttatcgtcat gccttgagtg gcaacatcct actatcggtc
    34381 tatttccagt ctcctgtgat acttaagaag atacaaactt ttaatggaaa aataaaattc
    34441 aggcagtttg tgggagacct acttgataaa ctatgcatgc ttgacaggga cggttttatt
    34501 tatggtcaaa atttaagact ctgtacagaa gccagatata tttgtcctgt gcaacaaatt
    34561 atagataccc ccctgttacg caaactcaac cagtttcgta cgtttgttcg taatgtccgc
    34621 ccgggtgatg aactggatgt cccggcacaa gttagtgaaa aaaatttaac cccgccgccg
    34681 ggtaacagca gtggcaacct tgagcaacag atagccagta cttcacagct aattggatct
    34741 ctgctcgccg aagatatgaa cagcgagcaa gcggcaaaca tagcacgtgg atgggcctct
    34801 tctcaggctt caggggtaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg
    34861 ctgggcgtgg atgaagattt cagcctgaag aactcccgtg acggcaaccc tcgaaaatgg
    34921 cgattcgatg caacaaacag tgaactatgt gccgaacgtc acgaatgctg aaatcacgct
    34981 ggcagcctcg aaggatccgg tgattgccga caataacgat ctcacgacac taacagcacc
    35041 gtcgctgata cagagggcaa tgcgatagcc aacactgagg taacatttac tctaccggaa
    35101 gatgtgaagg cgaacttcac gctgagcgat ggcgacaaag cggttactga tgccgatggc
    35161 aaagcgaaag tcacgctgaa aggtacaaaa gcaggcgctc ataccgtgac agcatcgatg
    35221 gttggcggta agagtgagca gttggtggtg aactttactg cggatacgct cactgcgcag
    35281 gttaatctta acgttaccga ggacaatttc atcgccaata acattggaat gaccaggcta
    35341 caggcaacag tgactgatgg aaacggcaac ccagtagaag gtataaaagt taatttccgc
    35401 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcaggt tttcgctgaa
    35461 attcttgtga caagcaccga ggtcggacta aaaacagttt cagcctctct ggcagataaa
    35521 cctactgaag tcatctcgcg attactgaat gccaaggtag atgttaattc tgcgacgatt
    35581 accagtcagg agatacctga aggtcaggtc atggtcgcac aagacatagc agttaaagct
    35641 cacgttaacg accagtttgg caacccggtg acgcatcaac ccgcgacatt cagtgcagcg
    35701 ccatcctcgc aaatgatcat cagccagaat acggtctcta ctaatacgca gggagtagcc
    35761 gaggtcacca tgacgcctga aagaaacggc tcgtatactg taaaagcatc cctagcgaat
    35821 ggagcttcac ttgagaaaca actggaggct attgatgaaa aactgacact cacgtccagt
    35881 ccgcttatcg gtgtcaatgc tcctaaaggc gctactctga cggcaacgct aacctctgca
    35941 aatggcactc cagtggaggg tcaggtcatc aactttagcg taacgctaga aggtgcgacg
    36001 ttaagtggcg gaaaagtgag aaccaactct tcaggtcagg ctccagtcgt tctgaccagc
    36061 aataaagtcg gtacatatac ggtgactgca tcgttccata acggcgtcac aatacagaca
    36121 cagacaaccg tgaaggttac tggcaaccca agcaccgcac atgttgctag ctttatcgct
    36181 gatccgtcga ctatcgccgc caccaacagt gatttaagta ccttaaaggc aacggttgaa
    36241 gatggcagtg gtaacctgat cgaaggtctc actgtgtact tcgccttaaa aagcggctcc
    36301 accacattaa cgtcattaac agcggtgacc gatcaaaacg gaatcgcgac aacaagcgtg
    36361 aaatgtgaga taacggggag cgtcactgta agcgcagtca caagcgcagg tggaatgcaa
    36421 acagtagata tatcgctagt ggcaggccca gcagacgcct cgcagtccat ccttaagaac
    36481 aaccagtcat cactgaaagg ggactttacc gatagtgctg agctacatct tgttctgcac
    36541 gatatatcag gcaatccgat caaagtttct taagggatag aatttgtgca atcaggtaca
    36601 aacgtgccct atatgaaaat tagcgcaatt gattacagtc aaaatatcaa cggtgattac
    36661 aaagctactg ttacaggagg cggagagggt atcgcaacgc tgatccccgt attgaatggt
    36721 gttcatcagg cgggtctgag taccacgata gaattcatta gcgcggaaac cagaccaatg
    36781 acgggtacag tgtcagtgaa tagtgcaaac ctaccgacag cttcattccc ttcgcaggga
    36841 tttaccgggg cgtattatca gttgaataat gacaactttg caccaggaaa aacggcggct
    36901 gattattcgt tttcaagctc ggcctcctgg gtaggcgttg atgctactgg taaagtgaca
    36961 ttcaaaaatg acggtgatag caatactgta ataatcacag cgcccccaag atccggtggt
    37021 gctatttacc aaacagttcc tcctgaatcg cgttcggtgt gactatgatc attttcggtg
    37081 agtattgctt tgatccaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
    37141 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgcct caaattctcg
    37201 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
    37261 caacgacgac tgactccgaa acatcggcca ttgcgggcca tcgcgttttg taatcagcat
    37321 aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag
    37381 ttattgcaat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtgataagg
    37441 ataaacggta gacattatta taacaatcca ctaatgccct agctttatct tcccctttgg
    37501 gtccataaac gatcactatt ggaatatctg tttcactttg aatttttgct attagatttt
    37561 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc gtctaaattg agcgcaatgt
    37621 aagatcctaa cgatcgcatt tcctcgcgta cctcatcgag tacatcatcg cttaatggca
    37681 attcatatat tgacctgact gccggaaaac ccgcctcacg catcataaat gcccatgtca
    37741 tgggtacagg agcccggaga ttctgatcca tccgggacgc gttcttacac aaaggggaat
    37801 agcacttcat ggttaaaccg acgacctgga aattcgtttt tgctttcaac tgactgataa
    37861 atatcatcgt tttcaggttc tttttacgca tcgcctctat acaaagatcc ggcgtaccgt
    37921 attgcagtgt tatgttcttc gctaaatctt ttatttcttt taatgttgcg tgatcctgca
    37981 tagtcattgt gactaatgtt aatttagtct gttcaagttt gagcgcatta aagacttcta
    38041 aattaattgt cgacgtaaca attaatagat gcttaatttt atgcaattca atcgcccgaa
    38101 taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatgtatg acaacaaagt
    38161 ctgttttttc aatattaaaa ttataagttt tataatcgta gtaactaaat gcaatacgtc
    38221 tcaacaatga tactaaaaac ataactaacc tcgcctccct actggttata atgcaatgca
    38281 gtctatcaga atcatcaggg tgcaattttg tgcatatgcg gacttttatg tttcatatct
    38341 cgaacctgtg ggccctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc
    38401 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
    38461 gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca
    38521 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
    38581 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagtttc
    38641 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcattaccgc cgcactggtg
    38701 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgcc
    38761 acctttggtg tcggcttcct tttccgaccg ctcggcggtg tcattttcgg tcactttggc
    38821 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
    38881 gccttgattg gcattcttcc ttccttctcg actattgggt ggtgggcccc cattttactg
    38941 gtgacgctgc gtgccataca gggatttgcc gtcggcggcg aatggggagg cgcagcgtta
    39001 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
    39061 ggctacggag taggtttact gctttcgacc ggactggttt cattgatcag tatgatgacg
    39121 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
    39181 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
    39241 caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct gttacgacat
    39301 cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac gatgtacatt
    39361 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg cgaacttttc
    39421 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
    39481 cttgccgatc gttttggtcg gcgcagggtt tatatcactg gcgcgttgat cggaacgttg
    39541 agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat agttttcttc
    39601 tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca accgatgttt
    39661 accgaaatgt ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc
    39721 agtgtggttg gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc
    39781 gggaactggc atagcgtcgc catttatttg ctggctggat gcctgatttc tgcaatgacc
    39841 gctttattga tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc
    39901 gccttatact tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg
    39961 aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag gcactggata
    40021 aactcgacgc gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata
    40081 ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt gtctatccat
    40141 cactgaccgt aacctgggac ggtagcacaa ctaatccccc caaaacgcgc gcatttggtc
    40201 gttttaccca cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt
    40261 atctcaatga acaacttacg ttgctgtatc aggattatgg tgcgcatatc tcggtgcaac
    40321 cctcgcagca tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc
    40381 gctcaatgag cgctgggtta actcgctact tcccgacaac agaactggcg caaattggcg
    40441 atgaaactgc agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg
    40501 cgcgccgcgt cgatttttcc ctcgcgcggt tgcgccatta taccggtacg ccagttgaac
    40561 attttcagcc gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt
    40621 ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg
    40681 ggaactggat caccgccgaa accgaagcgc cagaggaagc catttccgac cttgcatgga
    40741 aaaaacacca gatgccagca tggcatttaa ttaccgccga tggtcagggt attactctgg
    40801 tgaatattgg cgtgggaccg tcaaatgcaa aaaccatctg cgatcatctg gcggtgctac
    40861 gcccggatgt ctggttgatg attggtcact gtggtggctt acgtgaaagc caggccattg
    40921 gagattatgt acttgcacac gcttatttac gcgatgacca cgttcttgat gcggtactgc
    40981 cgcccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacta
    41041 agttagtcag cggcaggccc ggtgaggaag ttaaacagcg actacgtact ggcactgtgg
    41101 taaccacaga tgacaggaac tgggaattac gttactcagc ttctgcactt cgttttaact
    41161 taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt
    41221 tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttgcat ggcgagatta
    41281 aacttcccgg ccaggctaac cgtttttatg aaggtgctat ttccgaacac ctgcaaattg
    41341 gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattcg cgtaaattac
    41401 gaacctttaa tgagccgccg ttccgataat aagttctgtt cctttttatc aggaatagat
    41461 gtgaaggatt tagtaagtca tccagcaaat agcctgcaat aaagcaggct gtttgcttcc
    41521 tggggcggat acgagtttta ttatagcctt aatgatttcc acatattaaa agcaagtatg
    41581 ctttcaaaac acaattataa aaaaatccgc
//
LOCUS       DAZKHK010000028        41574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKHK010000028 DAZKHK010000000
VERSION     DAZKHK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.028"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            322..2715
                     /gene="malP"
                     /locus_tag="ACHOS5_001530"
     CDS             322..2715
                     /gene="malP"
                     /locus_tag="ACHOS5_001530"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP9776356.1"
                     /translation="MSQPIFNDKQFQEALSRQWRRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AVDISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSGGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2725..4809
                     /gene="malQ"
                     /locus_tag="ACHOS5_001531"
     CDS             2725..4809
                     /gene="malQ"
                     /locus_tag="ACHOS5_001531"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP9776357.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTTLKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(4920..6236)
                     /gene="gntT"
                     /locus_tag="ACHOS5_001532"
     CDS             complement(4920..6236)
                     /gene="gntT"
                     /locus_tag="ACHOS5_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9776358.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6600..7175)
                     /gene="nfuA"
                     /locus_tag="ACHOS5_001533"
     CDS             complement(6600..7175)
                     /gene="nfuA"
                     /locus_tag="ACHOS5_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP9776359.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7234..7917)
                     /gene="gntX"
                     /locus_tag="ACHOS5_001534"
     CDS             complement(7234..7917)
                     /gene="gntX"
                     /locus_tag="ACHOS5_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP9776360.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7955..8725
                     /gene="bioH"
                     /locus_tag="ACHOS5_001535"
     CDS             7955..8725
                     /gene="bioH"
                     /locus_tag="ACHOS5_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP9776361.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            complement(8754..9347)
                     /locus_tag="ACHOS5_001536"
                     /pseudo
     CDS             complement(8754..9347)
                     /locus_tag="ACHOS5_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(9580..9816)
                     /gene="feoC"
                     /locus_tag="ACHOS5_001537"
     CDS             complement(9580..9816)
                     /gene="feoC"
                     /locus_tag="ACHOS5_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP9776362.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            complement(9827..12148)
                     /gene="feoB"
                     /locus_tag="ACHOS5_001538"
     CDS             complement(9827..12148)
                     /gene="feoB"
                     /locus_tag="ACHOS5_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP9776363.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            complement(12165..12392)
                     /gene="feoA"
                     /locus_tag="ACHOS5_001539"
     CDS             complement(12165..12392)
                     /gene="feoA"
                     /locus_tag="ACHOS5_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP9776364.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(12830..15151)
                     /gene="yhgF"
                     /locus_tag="ACHOS5_001540"
     CDS             complement(12830..15151)
                     /gene="yhgF"
                     /locus_tag="ACHOS5_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9776365.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVAADTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(15248..15724)
                     /gene="greB"
                     /locus_tag="ACHOS5_001541"
     CDS             complement(15248..15724)
                     /gene="greB"
                     /locus_tag="ACHOS5_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP9776366.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            15952..16671
                     /gene="ompR"
                     /locus_tag="ACHOS5_001542"
     CDS             15952..16671
                     /gene="ompR"
                     /locus_tag="ACHOS5_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP9776367.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            16668..18020
                     /gene="envZ"
                     /locus_tag="ACHOS5_001543"
     CDS             16668..18020
                     /gene="envZ"
                     /locus_tag="ACHOS5_001543"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP9776368.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGMTKEG
                     "
     gene            complement(18096..19718)
                     /gene="pckA"
                     /locus_tag="ACHOS5_001544"
     CDS             complement(18096..19718)
                     /gene="pckA"
                     /locus_tag="ACHOS5_001544"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9776369.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            20097..21821
                     /gene="yhgE"
                     /locus_tag="ACHOS5_001545"
     CDS             20097..21821
                     /gene="yhgE"
                     /locus_tag="ACHOS5_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9776370.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIIILPFTGLSAISRHISAAGLLLTLAFLQLILMAIVLDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFAAAWALWLRVAQYGWTADRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLVVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     DDERYIVYGFDSARKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(21919..22116)
                     /locus_tag="ACHOS5_001546"
                     /pseudo
     CDS             complement(21919..22116)
                     /locus_tag="ACHOS5_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            22124..23332
                     /locus_tag="ACHOS5_001547"
     CDS             22124..23332
                     /locus_tag="ACHOS5_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9776371.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSCQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHFCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDPGWYEMRRQLEHKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(23455..24333)
                     /gene="hslO"
                     /locus_tag="ACHOS5_001548"
     CDS             complement(23455..24333)
                     /gene="hslO"
                     /locus_tag="ACHOS5_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9776372.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(24358..24759)
                     /gene="hslR"
                     /locus_tag="ACHOS5_001549"
     CDS             complement(24358..24759)
                     /gene="hslR"
                     /locus_tag="ACHOS5_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP9776373.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(24770..25438)
                     /gene="yrfG"
                     /locus_tag="ACHOS5_001550"
     CDS             complement(24770..25438)
                     /gene="yrfG"
                     /locus_tag="ACHOS5_001550"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP9776374.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(25503..27638)
                     /gene="igaA"
                     /locus_tag="ACHOS5_001551"
     CDS             complement(25503..27638)
                     /gene="igaA"
                     /locus_tag="ACHOS5_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP9776375.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFMMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            27958..28518
                     /gene="nudE"
                     /locus_tag="ACHOS5_001552"
     CDS             27958..28518
                     /gene="nudE"
                     /locus_tag="ACHOS5_001552"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP9776376.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(28685..31237)
                     /gene="mrcA"
                     /locus_tag="ACHOS5_001553"
     CDS             complement(28685..31237)
                     /gene="mrcA"
                     /locus_tag="ACHOS5_001553"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP9776377.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            31357..32136
                     /gene="hofM"
                     /locus_tag="ACHOS5_001554"
     CDS             31357..32136
                     /gene="hofM"
                     /locus_tag="ACHOS5_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP9776378.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            32136..32675
                     /gene="hofN"
                     /locus_tag="ACHOS5_001555"
     CDS             32136..32675
                     /gene="hofN"
                     /locus_tag="ACHOS5_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP9776379.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            32659..33099
                     /gene="hofO"
                     /locus_tag="ACHOS5_001556"
     CDS             32659..33099
                     /gene="hofO"
                     /locus_tag="ACHOS5_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP9776380.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQWAALYRLVDTTSFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            33089..33493
                     /gene="hofP"
                     /locus_tag="ACHOS5_001557"
     CDS             33089..33493
                     /gene="hofP"
                     /locus_tag="ACHOS5_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP9776381.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDAYTRRTGGKAAKSDADGG"
     gene            33405..34643
                     /gene="hofQ"
                     /locus_tag="ACHOS5_001558"
     CDS             33405..34643
                     /gene="hofQ"
                     /locus_tag="ACHOS5_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP9776382.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            35044..35565
                     /gene="aroK"
                     /locus_tag="ACHOS5_001559"
     CDS             35044..35565
                     /gene="aroK"
                     /locus_tag="ACHOS5_001559"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP9776383.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            35622..36710
                     /gene="aroB"
                     /locus_tag="ACHOS5_001560"
     CDS             35622..36710
                     /gene="aroB"
                     /locus_tag="ACHOS5_001560"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9776384.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLCLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            36802..38088
                     /gene="damX"
                     /locus_tag="ACHOS5_001561"
     CDS             36802..38088
                     /gene="damX"
                     /locus_tag="ACHOS5_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP9776385.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKMQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            38195..39031
                     /gene="dam"
                     /locus_tag="ACHOS5_001562"
     CDS             38195..39031
                     /gene="dam"
                     /locus_tag="ACHOS5_001562"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9776386.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            39049..39726
                     /gene="rpe"
                     /locus_tag="ACHOS5_001563"
     CDS             39049..39726
                     /gene="rpe"
                     /locus_tag="ACHOS5_001563"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9776387.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            39719..40477
                     /gene="gph"
                     /locus_tag="ACHOS5_001564"
     CDS             39719..40477
                     /gene="gph"
                     /locus_tag="ACHOS5_001564"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP9776388.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            40470..41474
                     /gene="trpS"
                     /locus_tag="ACHOS5_001565"
     CDS             40470..41474
                     /gene="trpS"
                     /locus_tag="ACHOS5_001565"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9776389.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
BASE COUNT         9918 a        10505 c        11282 g         9869 t
ORIGIN      
        1 taagaatgag atggagcaca tttgatattt tacttgtgaa actgtttcac tcctttactt
       61 aatttataga gttaccttcc gctttttgaa aatacgcaac ggccattttt tgcacttaga
      121 tacagatttt ctgcgcggta tggcattgat ttgctgctaa tcctgtggat tgcactagct
      181 ttaagtggtc gagatcacat ttccttgctc atccccgcaa ctcctccctg cctaatcccc
      241 cgcaggatga ggaaggtcaa catcgagcct ggcaaactag cgataacgtt gtgttgaaaa
      301 tctaagaaaa gtggaactcc tatgtcacaa cctattttta acgataagca atttcaggaa
      361 gcgctttcac gtcagtggcg gcgttatggc ttaaattctg cggctgaaat gactcctcgc
      421 cagtggtggc tggcagtgag cgaagcactg gccgaaatgc tgcgtgctca gccattcgcc
      481 aagccagtgg cgaatcagcg acatgttaac tacatctcaa tggagttttt aattggtcgc
      541 ctgacgggca acaacctgtt gaatctcggc tggtatcagg atgtacagga ttcgttgaag
      601 gcttatgaca tcaacctgac tgacctgttg gaagaagaga tcgacccggc gctggggaac
      661 ggtggtctgg gacgtctggc ggcgtgcttt ctcgactcaa tggcaactgt cggtcagtct
      721 gcgacgggtt acggtctgaa ctatcaatat ggtttgttcc gccagtcttt tgtcgatggc
      781 aaacaggttg aagcgccgga tgactggcat cgcggtaact acccgtggtt ccgccacaac
      841 gaagcactgg atgtgcaggt agggattggc ggtaaagtga cgaaagatgg acgctgggag
      901 ccggagttta ccattaccgg tcaagcgtgg gatctccccg ttgtcggcta tcgtaatggc
      961 gtggcgcagc cgctgcgtct gtggcaggcg acgcatgcgc atccatttga tctgactaaa
     1021 tttaacgatg gtgatttcct gcgtgccgaa cagcagggca tcaacgcgga aaaactgacc
     1081 aaagttctct atccaaacga taaccatact gccggtaaaa agctgcgcct gatgcagcaa
     1141 tacttccagt gtgcctgttc ggtagcggat attttgcgtc gccatcatct ggcggggcgt
     1201 aaactgcacg aactggcgga ttacgaagtt attcagctga acgataccca cccaactatc
     1261 gccattccag aactgctgcg cgtgctgatc gatgagcacc agatgagctg ggatgacgcc
     1321 tgggccatca ccagcaaaac tttcgcttac accaaccata ccctgatgcc agaagcgctg
     1381 gaacgctggg atgtgaaact ggtgaaaggc ttactgccgc gccacatgca gattattaac
     1441 gaaattaata ctcgctttaa aacgctggtg gaaaaaacct ggccgggcga tgaaaaagtg
     1501 tgggccaaac tggcggtggt gcacgacaaa caagtgcata tggcgaacct gtgtgtggtt
     1561 ggcggtttcg cggtgaacgg tgttgcggcg ctgcactcgg atctggtggt gaaagatctg
     1621 ttcccggaat atcaccagct atggccgaac aaattccata atgtcaccaa cggtattacc
     1681 ccacgtcgct ggatcaaaca gtgcaacccg gcactggcgg ctctgttgga taaatcactg
     1741 aaaaaagagt gggctaacga tctcgatcaa ctgatcaatc tggaaaaatt cgctgatgat
     1801 gcgaaattcc gtcagcaata tcgcgagatc aagcaggcga ataaagtccg tctggcagag
     1861 tttgtgaaag ttcgtaccgg tattgagatc aatccacagg cgattttcga tattcagatc
     1921 aaacgtctgc atgagtacaa acgccagcat ctgaatctgc tgcatattct ggcgttgtac
     1981 aaagaaattc gtgaaaaccc gcagtctgac cgcgtaccgc gcgtcttcct cttcggcgcg
     2041 aaagcagcac cgggctacta cctggcgaaa aatattatct ttgcgatcaa caaagtggct
     2101 gacgtgatca acaacgatcc gctggttggc gacaagttga aggtggtgtt cttgccggat
     2161 tattgtgttt cggcggcgga aaaactgatc ccggcggtgg atatctccga acaaatttcg
     2221 actgcaggta aagaagcttc cggtaccggc aatatgaaac tggcgctcaa tggtgcgctt
     2281 actgtcggta cgctggatgg tgcgaacgtt gaaatcgctg agaaagtcgg tgaagaaaat
     2341 atctttattt ttggtcatac cgtggaacaa gtgaaggcaa ttctggccaa aggctacgac
     2401 ccggtgaaat ggcggaagaa agataaggtg ctggacgcag tattgaaaga gctggaaagc
     2461 ggtaaataca gcggcggcga taagcatgcc ttcgaccaga tgctgcacag tatcggcaaa
     2521 cagggcggcg atccgtatct ggtgatggca gatttcgccg cttatgtaga ggcacaaaag
     2581 caggtggatg tgctgtaccg cgaccaggag gcctggactc gcgcggcgat cctcaatacc
     2641 gcccgctgcg gtatgtttag ctcggatcgc tctattcgcg attatcaggc tcgtatctgg
     2701 caggcaaaac gctaaggaag ctcgatggaa agcaaacgtc tggataatgc cgcgctggcg
     2761 gcggggatca gccccaatta catcaatgcc cacggtaaac cgcagtcgat tagcgccgaa
     2821 accaaacggc gtttgcttga cgcgatgcat caacgtaccg ccacgaaagt ggcggtaacg
     2881 ccagtcccga atgtcatggt ttataccagc ggcaaaaaaa tgccgatggt ggtggagggc
     2941 agcggcgaat atagctggct gctgaccacc gaagaaggaa agcagtacaa aggccatgtg
     3001 acggggggca aagcgttcaa tctaccgacg aagctgccgg aaggttatca cacgctgacg
     3061 cttacccagg acgaccagcg cgcgcattgc cgggtgattg tcgccccgaa acgctgttac
     3121 gaaccgcagg cgttgctgaa taaacaaaag ctgtggggtg cctgcgttca gctttatacg
     3181 ctgcgatcgg aaaaaaactg gggtattggg gattttggcg atctcaaagc gatgctggtg
     3241 gatgtggcaa aacgtggcgg gtcgtttatt ggtctgaacc cgattcatgc gctctatctg
     3301 gcaaatccgg aaagtgccag cccgtacagc ccgtcttctc gccgttggct gaatgtgatt
     3361 tatatcgacg ttaacgcagt tgaagatttc catcttagcg aagaggctca ggcctggtgg
     3421 cagttgccga ccacgcaaca gacgctgcaa caggcgcggg atgccgactg ggttgattac
     3481 tccacggtta ccaccctaaa aatgacagca ttacgaatgg cgtggaaagg tttcgcgcaa
     3541 cgtgatgatg agcagatgac cgcgtttcgc cagtttgttg cagagcaggg cgacagcctg
     3601 ttctggcagg cagcctttga tgcgctacat gcccagcaag tgaaagagga cgaaatgcgc
     3661 tggggctggc cagcatggcc agagatgtat cagaacgtgg attcaccaga agtgcgccag
     3721 ttctgcgaag aacatcgtga tgacgtcgat ttttacctct ggttgcagtg gctggcttac
     3781 agccagtttg ccgcctgctg ggagataagc cagggctatg aaatgccgat tggcttgtat
     3841 cgtgatctgg ccgttggtgt agcagaaggt ggggcagaaa cctggtgcga ccgtgaactg
     3901 tattgcctga aagcgtcggt tggcgcgccg ccggatatcc tcggcccgtt ggggcagaac
     3961 tggggattgc cgccaatgga cccgcatatc atcaccgcgc gtgcctatga accgtttatc
     4021 gagctgttgc gtgccaatat gcaaaactgc ggcgcattac gaattgatca cgtgatgtcg
     4081 atgctgcgtt tgtggtggat accgtatggc gagacggcag atcagggcgc gtatgttcac
     4141 tatccggtgg atgatctgct ctcgattctg gcactcgaaa gtaaacgtca tcgctgtatg
     4201 gtgattggtg aagatctcgg taccgtaccg gtagagattg tcggtaagct gcgcagcagc
     4261 ggtgtgtact cctacaaagt gctctatttc gaaaacgacc acgagaagac attccgcgca
     4321 ccgaaagcgt acccagagca gtcgatggcg gttgcggcga cacacgacct gcctacgcta
     4381 cgcggctact gggagagtgg ggatttaacg ctgggcaaaa cgctggggct gtatccggat
     4441 gaagtggtac tgcgcggtct gtatcaggat cgcgaactgg cgaagcaagg gctgctggat
     4501 gctctgcata aatatggttg cctgccaaaa cgtgccgggc ataaggcatc gttgatgtcg
     4561 atgacaccga cgctgaatcg tggcttacag cgctacatcg ccgatagcaa cagcgccctg
     4621 ttagggctac agccggaaga ctggctggat atggcagagc cggtaaatat tcccggcacc
     4681 agttaccagt ataaaaactg gcgacgtaaa ctttccgcaa cgcttgagtc gatgtttgcc
     4741 gatgatggtg tgaacaaatt gctgaaggat ttggacagac ggcgcagagc ggcagcgaag
     4801 aagaagtaga gtgcggttga tgccggatgc gacgcttgac gcgtcttatc cggcctacta
     4861 attaatgccg aatcgtaggc cggataaggc gttcacgccg catccggcca tcagtattat
     4921 caaatcacca tattcagcag cagacagcct accagaccgc acaccgagat aatggtttcc
     4981 agcatcgacc aggatttgat ggtctcgccg atagtcaggt taaagtactc tttgaacagc
     5041 cagaagcccg gatcgttcac gtgagagaaa atcacactac cggaaccaac cgcaataacc
     5101 atcagctccg ggctaacacc cgtcgttgca atcagcggtg ccgcgatacc acccgcagtg
     5161 attgccgcga cggttgcgga acccagcgcg atacgcagta cggcagcaat cgaccaggcc
     5221 atcagcagcg gagaaatgtt ggtttcgtgc atcatggaag caatgtattt gtccacgccg
     5281 ctgtctacca gcacctgctt gaacgcaccg ccaccaccga taatcaacag catcatcgct
     5341 atgattttga tggaagaaac cagcgtgtcg ttaatctgat ccattgaacg accacggttc
     5401 agaccaaagg tgaacatcgc aatcagcacg gcaatcagcg tggccattac cgggtcaccg
     5461 aggaactccg ccaccggcag gaaagcgtga cctttcggca ggatcatttc ggcaatcgca
     5521 cgcatcgcca tcagaaccac cggcaccaga gaggtccaga cgctgacgcc aaagctcggc
     5581 atctcttctt cgctgaaagt tttcgcgctg tacagacctt ccgggatcgg cttatcgata
     5641 cctttcagca cgcgagcgta aaccggaccg gcgagaatca cggtcgggat tgccagaata
     5701 gtaccgtaca gcagagtttt acccatatcg gcattgaaaa tggtggcaat tgcagtcgga
     5761 cccggatgcg gtggcaggaa gccgtgggtc acagacagtg cagccgccat tggtacacca
     5821 acatacagca gtggaatatt cgcggaagcc gcgatggtaa acaccagcgg cagcatcagc
     5881 acgaaaccca cttcatagaa cagggcaaaa ccaacggtaa aaccggtcag taccaccgcc
     5941 cactggatgt gttttttacc aaatttggca atcagtgtgg tagcaatacg ttgtgcacca
     6001 ccgcagtctg ccagcatttt gcccagcatc gcaccaaaac ccatgatcag ggcaaggcta
     6061 ccgagcgttc cgccgacacc agctttgatg gagccaataa ctttatccag cggcattcct
     6121 tgcattaatc caacagcaag cgccacgagg acgagagcga tgaagccgtt cattttgaag
     6181 cggatcatca ggagtaataa caagattaca ccgatagcaa caatgactaa tggcatgatt
     6241 tacctggcct ttaatttgtt atgggtaacg tcaggtttct gacggaaaat cctggttgtc
     6301 ccaactggga acagagatgt tacggcacca cggctgatga cccctaaaac gagttagtgt
     6361 agtcagatgt tatgagtgtg tttggtgccc atgagaatga tacgggtaac attgcgtgct
     6421 tgagaatcac cagagcgggg taaatttaaa ttatgagagg ttggtcatat tatcgtgggg
     6481 aaacgaagag attggatttg ctgagataat tctgcgctaa cgtcagactc tcgtgcaacg
     6541 tacaggcccg ataagacgct ttagcgtcac atcgggcaat cgacaaaagg ggagagaact
     6601 tagtagtaag agtgctcgcc gcgttggtgt tcggtgagat cgcgtacacc tttcagctcc
     6661 gggaattcgt tcagcaactg cttttcgatc ccttctttca gggtgacgtc gaccatggaa
     6721 caaccgttac agccaccgcc aaattgcaaa atggcgtaac cgtcttcggt gatttccatc
     6781 agcgaaacgc gaccaccgtg accagcaagc tgtgggttga tctgtgattg cagcatatac
     6841 tccacgcgct ccatcagcgg cgcatcgtca gccactttac gcattttggc gttaggcgct
     6901 ttcagcgtca gctgcgaacc caactggtcg gtaacaaaat cgatctctgc atcttccagg
     6961 taaggagcgc ttaactcatc aacatacgcg gtcagcaggt caaatttcag ggcggtgtcg
     7021 gtggcttcca cagcgtccgg cgggcaataa gaaacaccac attcagcgtt aggcgtgcca
     7081 gggttaatca caaatacgcg gatttgtgtc ccttcttcct gatttgccag cagtttggca
     7141 aagtgcgctt gtgcagcatc ggaaatacgg atcatagtaa tggcctaata gttgactatt
     7201 ttagttggtt ataatacgcc catcatcgag gctctacaag gttcgacaaa ggcaccagac
     7261 ctggacagtc gccgcaccat tgcgtaaaag caactgcgca atctctgcga cggtacttcc
     7321 ggtggtaacg acatcatcca caatcaccat atggcgacct tgcacaggca attcaaggcg
     7381 aaaggcattt ttcaggttgt gtttgcgtag ccgggcgtta agaaaatgct gggtcgcggt
     7441 ggcccgtgtt cgtgtgacgg cttcgctatc ccattggcag tgtagccagc gtgataacgg
     7501 ctgacacagc aaatcgctct gattaaatcc ccgacgccag tgacggcgct gccataacgg
     7561 aacgctgatg atgcgatcag gcagttgcaa gccggtggtg cgacgagcgt gtaagacttc
     7621 caatagtaac agacgtgaca gggcgctggc gatttcactg cgccgggaaa acttaagctg
     7681 gtgaatgagc ggacttaatg gcggcgcata gtcggcgacc gtgaccagtc tttgccaggg
     7741 cagcggtttt tgcaggcagc gaccgcaggg aagatgagag tgtgtggcgg gtaatccaca
     7801 ttgtgggcat aacgttttat ctgtacgggc ggcgcgagag cagaccgaac aaatccccca
     7861 atgacctaac gccagtggca ttcggcatag ccagcataat cccggtactg ttagcatatg
     7921 ttcatccttg taagtaaaaa gagaacaata gcggatgaat aacatctggt ggcagaccaa
     7981 aggtcagggg aatgttcatc ttgtgctgct gcacggatgg ggactgaatg ccgaagtgtg
     8041 gcgttgcatt gacgaggaac ttagctcgca ttttacgctg caccttgttg acctgcccgg
     8101 cttcgggcgt agccggggat ttggtgcgct atcacttgct gatatggccg aagtcgtgct
     8161 gcgacaggca ccagataaag ccatttggtt aggctggagt ctgggcgggc tggtggcaag
     8221 ccagattgcg ttaacccatc ccgagcgggt tcaggcgctg gtcaccgtgg cgtcgtcacc
     8281 ttgttttagt gctcgcgacg agtggccggg gataaaaccg gacgtgctgg cgggatttca
     8341 gcagcaactc agtgatgatt ttcagcgtac agtggagcgg ttcctggcgt tacaaaccat
     8401 gggtactgaa acggcgcgcc aggatgcgcg ggcgttgaag aaaaccgttc tggcgttacc
     8461 gatgccggag gttgacgtgc ttaatggcgg gctggaaatc ctgaaaacgg tcgatctccg
     8521 tcagccgctg caaaacgtgc ccatgccgtt tttgcgattg tatggctatc tcgacggtct
     8581 ggtgccgcgc aaagtggtgc cgatgctgga taaactttgg cctcacagcg aatcatatat
     8641 cttcgccaaa gcggcccatg cgccgtttat ttcacatccg gacgagtttt gtcatctgct
     8701 ggtggcgttg aagcagaggg tgtaggtggc tttcgaaatg gcgggacaga actttactgg
     8761 ctcgccgcag ccaactcttc ttctgacacc ccggtaaagc gcatgatgtc tgcaagaggg
     8821 actccggatt caagcattat ttttgctata tgcaaggctt tggattgttc cccttcctgc
     8881 cgcaatcttt ccgcaatagt cattaaactc tccttgtgtt ttggtgaacg ttcggccaca
     8941 ccatcgataa aatcgttaaa acgtaccgcg tcgccggttt gcagtatgta attaaacagc
     9001 cctttgattt gtctgccatt agcgtatcca ctacttaata agcaggccat ttgctctacc
     9061 agccccatca ggtcgcgttg acgaatatgt ttttgaatta actccagtag cgccatacgt
     9121 cggtgcagca tgatttcatc atcaggcatg agggtgacat caatcagcgg aaatgcagag
     9181 gcataaagct gccttgccag gttgggatcg gcgaaacaat ccagccaaca cagcgaatag
     9241 ggatagaagc tttcaatacc gtgataaaac aacagtggca ccaccatcgg caatgttttg
     9301 tatccggcat caaggtgatt ttgcattgcg gcaatggcgt aacgcatcgt gtggggtgga
     9361 actctgcttt ttgctcatgg cgttccttca cctgttattt gaatgccgcg actctacgcg
     9421 ccactccaca cctgtgcatt agcgagtttt catccctgcg agacgctttc agaggaactt
     9481 acgtcgcgct gcaagggttg catttttatg gaaagccgca cgcaaaaaag cccggatgca
     9541 aacgcatacc gggctttcaa aacaggcagt gaaacaaggt taacgcaacg cccaccattc
     9601 gtgcagacag gcttttcctt ccgggcagct tttacaactg ccggaaagac agccgtcagg
     9661 ttcttcctga atccgcactg cttttcccat actttctagc tgtttcagca tggcgttaat
     9721 cattggctgc ggagtgttca gtgtctggct aatctgggcc gcttccatac ggccccgtaa
     9781 tgccagtaaa tcacgcactt gaatgagcga agccattgtc gtgtccttag tggcaatcgc
     9841 cagtggtact ggccgcacag caactgctta ccgatttgcg cgttgccagc agttcgacat
     9901 ctacccggct acgcgcgcgg cgcagcagac cgataaccac gatgttaaac aggataactg
     9961 ccagaatgca caccaggctg taagttggat gctggctgta gctggcgacc tgatagaaca
    10021 atgttgccag tgagtaagca atattcagcc cccacaggat ggagaagccc atccagccac
    10081 ggcttgattc acgcgcgata gcccccatca ccgagatgca cggtacgtac agcaggacga
    10141 aaatcaggta gctgtatgcg gcagctgcgc taccgaattt ctgatccatc acgcccatcg
    10201 ccccggtacc catttcgccg tcgcctttgc tggcttcaat ggggttcatc agtacgctaa
    10261 ggctgaaggt gtctttcagg ctctgccagg tttcatctac cgcgctgaac agctcttcac
    10321 cgaggttaaa ttccgccgga ttgaactctt cgtcctgaat attttctgcg gtgtagaggg
    10381 tgttgagcgt acccactacc acttctttcg ccatggcacc tgtaaacagg ccaaccgttg
    10441 cctgccagtt atcttcatgc acgccaatcg gcttgaagac tggggtgatc acccggctga
    10501 cggaagccag cgccgagtcg ttgatgttat cgacgatttt cccgctcaga gagaagctgt
    10561 tgaaagcact caggaaaatg ctgacgatga taatcacttt accagcacgc agaacgaagc
    10621 ctttcagacg ctgccaggtc tggataatca ggcttttaac gtgtggtaca tggtagaccg
    10681 gcagctccat gacaaacggc gtcgcttcac cacgcatgat ggtgtacttg agcatcaggc
    10741 cagtcagcac cgccatcacg atacccagca tatacagcga gaagaccgcc agcgcaccgt
    10801 tctgcccgaa gaaggcagcc gcgaatactg cgaagatagc cagacgcgcg ccgcaggaca
    10861 taaacggtgc catcatgatg gtcatcagac gttcacgcgg tgcatcaagc gtacgtgcgc
    10921 ccattaccga cggtacgtta caaccgaaac caacgatcag cggtacaaag gatttccccg
    10981 gcaagcccag cgcctgcatc agacggtcca tcacaaacgc cgcacgcgcc atatagccgg
    11041 agtcctcaag gaaggagagg aacaggtaca tcatgccgat ctgcggcacc agtggcagca
    11101 cggtgttaat accaccgccc aggccttggg cgaggaagat agtcagccag tccgggaagt
    11161 ggagcgtgta gccaatccac tgaataccat gcacaaatag tgctacggag ccgacgtcaa
    11221 acagcggctg taacgccccg ccgatgttga tagccagcaa gaacatcagg tacatcacaa
    11281 agaggaaaat cggcagaccg aggaaacggt tgagcacgat tttatctacc gcagtggtga
    11341 aacgactggg ttctgccgtc agggtgttgc ttaccacatc acagatggca gcaatgcact
    11401 ggtaacgcgc atcggcaatg tgcagcgccg gatcgtccat ctcattacgc agacgggcga
    11461 gggcggcatc cagatgctgc gacgcttcac cggcgtaggc gcggctgtag atatcgcctt
    11521 ccagcatttg caggcccagc cagcgacgtt gtttcagcgg gatgtcggaa ggcatcactt
    11581 ttgccagtga atctgcttcg ttgagcagcg gctgagcgta atgcaccagt tccacatttt
    11641 cgttagcttt atagcgatca atcgccagct tgagcgcttc aataccgcga ccacgggttg
    11701 aaaccagcgg gatcaccggg cagccaagac gcgccgacag cgcatcaatt tcaatgcgaa
    11761 tattttgctt ctcggcaatg tcgagcatgt tcagcgccac gatgcatgga atgccgagtt
    11821 ccaacagttg cagcgtcagg tacaggttac gctcaaggtt agacgcatcc accacgttaa
    11881 tcagcaggtc tgcgtcgcca ctcaaaatgt agtgacaggc gatttgctca tcgagcgagg
    11941 tctgcgatga gatggtggtc agagaatagg tgccgggcag gtccaccagc gtgacctgat
    12001 gatcggtggt ggagaattgc ccttctttac gttcgacggt aacgccagcc cagttaccta
    12061 cacgctgacg tgagccagtg agctggttaa ataacgttgt cttgccagaa tttggattac
    12121 caattaagcc aatggttaat tttttcattg ttgttatcac cgtattaaca ggaaaccgct
    12181 tccacttcta ataaggccag atcttttttg cgtaatacca ggctcacacg acgggtttcg
    12241 atatgaatgg ggtcgccgag tggagcgacg cgcaccacat taaaagagga gccaggtaac
    12301 atgccaagag aaagcagttt ttggcgatat gccgggctga tttcacggga aaagccagta
    12361 attttccacg cagtatctgg agtgtattgc ataggtgcct acttgtttct cattaactgg
    12421 ataactacct catggggcgt ttgacagcga catgagacca gatgatttgc caacgaagga
    12481 gagattaaat aatcaacagt acaatgataa tgagaatggt ttctatcagc aatacttaaa
    12541 atgtgtgtga atgttggttt gaataataat ctgactcaat gttgatatgg ctcaaggtta
    12601 tatcgcagcg aaaatataat tcgcatgacg aattattagg gctggttaat gtttaattaa
    12661 ggtttcagtt gttaatgaag tgataaatat taacggaatc tattatgagt ttgcttgtgg
    12721 aataaaaaga ccggagatat tctccggcac ttttatgtca ggcaaggcgt tcattgccag
    12781 atgcggcgtg aacgcctaat cgcgcaggtt taggggccag tccgggcgtt taacgttttt
    12841 tgcccattgc cgccgccagc gcatccatca tcgcgctatt accggcaggc tgcgcttcac
    12901 gcccacgcgg tttggctgcc gggcggttgt tttgcgggcg atcgttaccg ccaccgcgac
    12961 gggcgttggt ttcgccaggc tgctcgtcca gacgcatggt cagggcgata cgtttacgct
    13021 gaagatccac ttccagcact ttcaccttca caatgtcgcc cgctttcacc acggtatgcg
    13081 gatcttccac aaacttgttc gacaatgaag agatgtgaac caggccgtcc tgatgcacac
    13141 caatatcgac aaacgcgcca aagttggtga cgttggtcac cgcaccttcg aggatcatac
    13201 ccggttgcag gtcgttcatt gtctcgacgc catcggcaaa ctgagcggtt ttaaattccg
    13261 gacgcggatc gcgacccggt ttttccagct ctttgatgat gtcggttacc gtcggcacac
    13321 cgaatttctc atcagtaaag tcagatgctt tcaggttacg cagttcgctg ctgttaccca
    13381 tcagatcttt cagtgcctgc tgtgttgctg ccagaatgcg ttccaccacc ggataggctt
    13441 ccgggtgaac ggtagaagcg tccagcgggt tatcaccgtg gttaatgcgc aagaagcccg
    13501 cgcactgctc gaaggctttc ggccccagac ggctcacttt taacagttgc tgacggttct
    13561 ggaactggcc gttctcatcg cgccaggcca cgatgttttg cgccatcatg cgcgtcaggc
    13621 ccgccacgcg ggttaataga ggaacagaag cggtgttgag atcgacgcca acggcgttta
    13681 cgcagtcttc tactactgca tccagtttgc gtgccagctg cgtctggctg acgtcatgct
    13741 gatactgacc tacgccgata gatttcggat cgattttcac cagctccgcc agcggatcct
    13801 gcaaacgacg ggcgatagac accgcgccac gcagcgaaac gtcgagatcc gggaattcct
    13861 gtgctgccag ctcggaagcc gagtaaaccg acgcgccagc ttcgctgacg atcactttct
    13921 gcgcggtcac tttcgggaac tgcttctgca cgtcgagata gaaacgttcg gtttcgcggg
    13981 aagctgtacc gttgccaatc gccaccagtt caacgttatg cttttcacac agcgcagcaa
    14041 cggtcatcgc tgcttttgcg gcctgcccgg tatgcggata aatggtatcg gccgccacca
    14101 gtttacctgt cgcatcgact accgccactt ttaccccggt acgcagaccc ggatcgaggc
    14161 ccatcgttgc acgcagtccg gcaggggccg ccatcagcag atcgtgcagg ttacgggcaa
    14221 agacgttgat tgcttcatct tccgcgcgtt cgcgcacggt gcccatcagt tcagtttcca
    14281 gatgcatcaa caccttgatg cgccacgtcc agctcaccac acctttgcgc cagctatccg
    14341 ccggggcatt gttcaggcgc aggccaaggt gatccatgat gatttgctcg caatagctct
    14401 ctttcggcgg ctcttcgaac tgcggatcgg cattcagcga aagctgaagt atgccttcgt
    14461 tacgaccacg gaacatcgcc agtgcgcggt gagaaggcac cgttgacagt ggttcgtgat
    14521 gatcgaaata gtcgcggaat tttgcccctt cctcttcttt accgctcacc acggttgaga
    14581 ccagatgcgc gttcttccac agataatcac gcactttcgc cagcagcgca gcatcttcgg
    14641 caaaacgttc catcaggata tagcgcgcgc cgtccagcgc ggctttggta tctgccacgc
    14701 ctttatcggc atcaatatat tgtgcagcgg cgacttctgg cgtgtgtgac ggatcgctcc
    14761 acagcagatc ggccaacggc tcaagccctg cttcaatggc gatttgccca cgggtgcggc
    14821 gtttaggttt gtagggcagg tagaggtctt cgagttcggt tttgcttaag gtggcgttga
    14881 tggcattcgc cagatcatcg gtgagtttgc cttgctcgga aatggacttg aggatcgcct
    14941 gacgtctctc ttccagctcg cgcagatagc tcagacgcgt ttccagatta cgcagctgcg
    15001 tgtcatccag accgccggtg atttccttac gataacgtgc gataaacggc acggtattcc
    15061 cttcgtcaag caggcgaacg gcagcgtcaa cctgttccgg gcgcgcctga atttcacccg
    15121 caataatgcg gcagaacgaa tcattcatca tggtttgagt tcatcttttc ggatcaaaaa
    15181 tcaggggata gttatacgga ctggctggca aaaatgccag ccatcggcag gaggttaaga
    15241 ctcttcctta cggtttcacg tactcgatag cattaacata ccagctcgct tccccggcag
    15301 gggtattcac caccgccaga tcgccgactt cttttttcag caatgcgcgg gccatcgggg
    15361 aatcgataga gatgtaatct ttacggccaa aaatttcatc gtagccgaca atacggaaac
    15421 ggtgagtcac gccatcgtcg ttttcaatct ccacccacgc gccaaaaaag actttgcctt
    15481 cctgctgcgg ggagtaatcg acgattttga gattttccat gcatttagtg agatagcgta
    15541 cgcgacggtc gatttcacgc aggcgctttt tattatactg atagtcagca ttttcgctgc
    15601 ggtcgcccag acttgcggcc caggtcactt tctttgtgac ctccgggcgt tcttcacgcc
    15661 agagataatt aagctcttgt ttgagttttt catacccttc ccgggtaacc aggggcgttt
    15721 tcatctcgtt gattcccttt gtctgtttga taatgcgcac attgggtata acgtgatcat
    15781 atcaacagaa tcaataatgt ttcgccgaat aaattgtata cttaagctgc tgtttaatat
    15841 gctttgtaac aatttaggct gaaattcata ccagatttag ctggtgacga acgtgagctt
    15901 ttttaagaat acacgcttac aaattgttgc gaacctttgg gagtacaaac aatgcaagag
    15961 aactacaaga ttctggtggt tgatgacgac atgcgcctgc gcgcgctgct ggaacgttat
    16021 ctcaccgaac aaggcttcca ggttcgaagc gtcgctaatg cagaacagat ggatcgcctg
    16081 ctgactcgtg aatctttcca tcttatggta ctggatttga tgttacctgg tgaagatggc
    16141 ttgtcgattt gccgacgtct tcgtagccag agcaatccga tgccgatcat tatggtgacg
    16201 gcgaaagggg aagaagtgga ccgtatcgtt ggcctggaga ttggcgctga cgattacatt
    16261 ccaaaaccgt ttaacccgcg tgaattgctg gcccgcattc gtgcggtgct gcgtcgtcag
    16321 gcgaatgaac tgccaggcgc accgtcacag gaagaggcgg taattgcttt cggtaagttc
    16381 aaacttaacc tcggtacgcg cgaaatgttc cgcgaagacg agccgatgcc gctcaccagt
    16441 ggtgagtttg cggtactgaa ggcactggtc agccatccgc gtgagccact ctcccgcgat
    16501 aagctgatga accttgcccg tggtcgtgaa tattccgcaa tggaacgctc catcgacgtg
    16561 cagatttcgc gtctgcgccg catggtggaa gaagatccgg cgcatccgcg ttacattcag
    16621 accgtctggg gtctgggcta tgtctttgta ccggacggtt ctaaagcatg aggcgattgc
    16681 gcttctcgcc acgaagttca tttgcccgta cgttattgct catcgtcacc ttgctgttcg
    16741 tcagcctggt gacgacttat ctggtggtgc tgaacttcgc gattttgccg agcctccagc
    16801 agtttaataa agtcctcgcg tacgaagtgc gtatgttgat gaccgacaaa ctgcaactgg
    16861 aggacggcac gcagttggtt gtgcctcccg ctttccgtcg ggagatctac cgtgagctgg
    16921 ggatctctct ctattccaac gaagctgccg aagaggcagg tctgcgttgg gcgcaacact
    16981 atgaattctt aagccatcag atggcgcagc aactgggcgg tccgacggaa gttcgcgtcg
    17041 aggttaacaa aagttcgcct gtcgtctggc tgaaaacttg gttgtcgccc aatatctggg
    17101 tacgcgtgcc gctaaccgaa attcatcagg gcgatttctc cccgctgttc cgttatacgt
    17161 tggcgattat gctattggcg ataggcgggg cgtggctgtt tattcgtatc cagaaccgac
    17221 cgttggtcga tctcgagcac gcagccttgc aggtgggtaa agggattatt ccgccgccgc
    17281 tgcgtgaata tggcgcttca gaggtgcgtt ccgttacccg cgcctttaac catatggcgg
    17341 ctggtgttaa gcaactggcg gatgaccgta cgctgctgat ggcgggggta agccatgatc
    17401 tgcgcacgcc gctgacgcgt attcgcctgg caaccgagat gatgagcgag caggatggct
    17461 acctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt gagcagttta
    17521 tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctc aacgcagtac
    17581 tcggtgaggt tattgctgcc gaaagtggct atgagcggga aattgaaact gcgctttacc
    17641 ccggtagcat tgaggtgaaa atgcacccgc tgtcgatcaa acgcgcggtg gcgaatatgg
    17701 tggtcaacgc cgcccgttac ggcaatggct ggatcaaagt cagcagcggc acggagccga
    17761 atcgcgcctg gttccaggta gaagatgacg gtccgggtat tgcgccggaa caacgtaagc
    17821 acctgttcca gccgtttgtt cgtggcgaca gtgcgcgcac cattagcggc acgggattag
    17881 ggctggcgat tgtgcagcgt atcgtggata accataacgg gatgctggag cttggcacca
    17941 gcgaacgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg cgggcgcagg
    18001 gcatgacaaa agaagggtaa aaaaacggga ggtgaaggtg cctcccgttt tgctttctat
    18061 aagatactgg atagatattc tccagcttca aatcattaca gcttcggacc agccgctacc
    18121 agcgcggcac ccgcaggggt gtcggtgtat ttatcgaagt tgtcgataaa cagtttcgcc
    18181 agcgtttcgg ctttttcctg ccactgttcc ggagaagcgt aggtgttacg cggatcgagg
    18241 atcttcgtgt ctacgcccgg cagttcggtt gggatcgcca ggttaaacat cggcagagtg
    18301 aaggtttctg cgttatccag cgaaccgttg aggatggcgt cgataatagc gcgggtatct
    18361 ttaatggaga tacgtttgcc agtgccgttc cagccggtgt taaccagata cgcctgcgca
    18421 cccgccgcct gcatacgttt taccagcact tctgcgtact gagttgggtg cagcgacagg
    18481 aacgccgcgc cgaagcaggc ggagaaggtt ggcgtcggtt cggtgatgcc acgctcagtc
    18541 ccggccagtt tggcggtgaa gccagagagg aagtggtact gggtttgatc ggcagtcaga
    18601 cgagaaaccg gcggcagcac accaaaggcg tctgctgtca ggaagataac tttcgttgcg
    18661 tggcccgctt tggaaaccgg cttaacgatg ttatcgatgt gatagatcgg ataagaaacg
    18721 cgggtgttct cggtttttga accatcatca aagtcgattg tgccatcttc acgcacggtg
    18781 acgttttcca gcagcgcatc gcgacggata gcgttgtaga tttcaggttc cgcttctttc
    18841 gacagcttga tagttttcgc gtagcagccg ccttcgaagt taaacacacc gtcatcatcc
    18901 cagccgtgct cgtcatcgcc aatcaggcgg cgtttcgggt cggtggaaag ggtggtttta
    18961 ccggtgccgg aaaggccgaa gaacaccgca acatcgcctt tctcgccaac gttggcggag
    19021 cagtgcatag aagcgatacc tttcagcggc agcaggtagt tcatcatcga gaacatccct
    19081 ttcttcattt cgccgccgta ccaggtgccg ccaatcagct gcatgcgctc ggtcaggtta
    19141 aacgccacga agttttcgga gttcagaccc tgttctttcc actgcgggtt agtgcacttc
    19201 gcgccgttca taacgataaa gtctggtttg aaacctgcca gctcttcatc gctcgggcga
    19261 ataaacatgt ttttgacaaa atgcgcctgc caggccactt cggtgatgaa acggacggaa
    19321 agacgagtat ccgggttcgc accacagaag gcgtcgacaa cgaacagacg tttgccggaa
    19381 agctgcctgg tcaccagacc tttcagatgc tgccaggttt ccggagagag aggtttgttg
    19441 tcgttcttac ctttgccttt gtctgcccac cagaaagtat cgcgagtggt atcgtcacga
    19501 acgatatact tatctttcgg tgaacgaccg gtgaagatcc cggtatcgac ggcaacggca
    19561 cccagattag ttaacacccc gcgctcataa ccggtcaggc tcgggtccag ctcttcctga
    19621 tacagcagat cgtagcttgg gttgtaaacg atatcatgta cgtcactgat accataagcc
    19681 tcgagttctt gcggggtcaa accattgtta acgcgcattt cactgctcct tagccaatat
    19741 gtattgcctg aatagtaaag tctttttggg ggtgttaacc gcgacaaggc tcatagattt
    19801 acgtatctgg agaaattcat atttgacaaa aaactccgtg attcctgtca cgaaacggtt
    19861 gctattatcg cagaaatagc attttaggtg tggaaaatgt tctcaaaagg ttaaatcttg
    19921 gtttatatgg cgggaatatt gtgagtggaa tcgcattcat gaatgaaatt gatgcacatt
    19981 caatcggtaa agtatcgatt caccacgcta actttttttg attatttttt gaaaaaagca
    20041 aaatactact gcccggatgc gataattaac ccaccttttg attctggata aacaacatgg
    20101 ataacgttga actttcaccg gcaacacgct gggggatgat tgctaccgga ttacttcagg
    20161 ggttggtttg ctacctgctg atcgcctggc ttgccggaaa aaatctcagt tggattgttt
    20221 atggcgtgcc tgcgaccgtc gcgttttcat ccgttttact cttctccgtg atctccttta
    20281 aacagaagcg cctttgggga tggctggcgc tggtgtttat tgccacgcta gggatgagcg
    20341 gttggctaaa gtggcaaatt gatggcatga acccctggag agctgaaaag gcgctttggg
    20401 attttggctg ctatctgctg ctgatggcaa tgttgttgct accgtggata caacaaagcc
    20461 ttcgcatccg caatgacagt agccgctatc gctactttta ccagtcagta tggcataacg
    20521 tacttatatt actggtgatt tttctcgcca atggcctgac gtggctggtg cttttactgt
    20581 ggagtgagtt gtttaaactt gttggcatca cgtttttcaa aacgctcttt tttgcaaccg
    20641 actggtttat ttatctcacg ttaggtctgg ttaccgcgct ggcggtgatc ctcgcgcgaa
    20701 cacagtcacg tttaatcgac tctattcaaa agttgttcac gttaatcgcc acggggttgc
    20761 tgccgttagt atcattgcta accctgatgt ttattatcat cctgccgttt acgggcctga
    20821 gcgcgatttc tcgccacatc tccgccgctg ggttgctgtt aacgctggcc tttttgcaat
    20881 tgatcttaat ggctattgtc ctcgatccgc aaaaagcgtc acttccctgg acggggccat
    20941 tgcgttgcct gattaaaacc gctttgctgg ttgctccgct gtatgtgttc gccgccgcct
    21001 gggcgctatg gctgcgggtc gctcagtacg gctggactgc cgaccgcttg cagggcgcgc
    21061 tggcggtgct ggtgttactg gtatggtcgc tggggtattt tgtcagtatc gtctggcgta
    21121 aagggcaaaa tccccttgat cttcagggca aagtgaacct tgtggtttcg ttattggtgt
    21181 tggtaatact ggtgcttctt aattcgccgg tgctggacag tatgcgcatt agcgtgaaca
    21241 gtcatatggc gcgttatcag agcggcaaaa acacgccaga ccaggtaact atctacatgc
    21301 tcgaacagag cggtcgctat ggacgtgcgg cgcttgagtc gctgaaaagc gatgccgagt
    21361 atatgaaaga cccgaaacgc gcgcgggatc tgctgatggc gttagatgga gagcaacatc
    21421 ttcaggaaca ggtatcggaa aaagtattag ccgataatgt gttaattgcc ccaggttctg
    21481 gtaaaccgga tgcgacattc tggtcggcct taatccagga tcgctataac gtgatgacct
    21541 gtattgaaaa agacgcctgc gtactggtcg agcaagatct gaatagtgat ggtcaggcgg
    21601 agcggatcct ctttgccttt gatgatgaaa gatacattgt ctatggcttt gactcagcca
    21661 gaaaagaatg ggacgcgctt gatatgagtt tacttccgcg caaaataacg aaagaaaaat
    21721 tactcacagc tgcgaaggat ggaaaactgg ggacgaggcc gaaagcgtgg cgcgatctga
    21781 cggtagatgg tgaaacgctg gaaataaact taagtaagtg acaacgcttt taacggatac
    21841 gacgctgtat ctgaccaaaa taacttgcga tttatccggt gcgcggtaaa accccgtcct
    21901 tcagggcggg gatataagtt cgccatccat ttcaaccagt tcagcttcaa aatcagcaca
    21961 tacatttgaa aagtaagtgc gtagtttttc tgtcgcgtca tggtcaaaaa tctggcgttg
    22021 gtatttggtg acaaagacca ggtgaacatg catcaggaaa acacaatgcc ttccacgccg
    22081 gatatcggtt tcttttttca tagaccaaaa tataatttga cctgtgaaac gactacaggc
    22141 atttaaattc cagttaagac ccggtggtca acaggagcgt gaaatgaggc gtttcgcagg
    22201 cgcatgtcgt ttcgttttca atcgtgctct ggcgcttcag aatgagaatc atgaggccgg
    22261 taataaatac atcccttacg ggaaaatggc ttcctggctg gtagagtgga aaaatgccac
    22321 tgaaacgcaa tggcttaaag attctccctc acagccattg caacagtcac tgaaagacct
    22381 tgagcgggct tacaaaaact tcttccagaa gcgggctgct tttccccgat tcaaaaagcg
    22441 gggacagaat gatgcattcc gctacccgca gggcgttaag ctcgatcagg aaaacagccg
    22501 tatttttctg ccgaaactgg gttggatgcg ctaccggaat agctgtcagg tcacgggagt
    22561 tgtgaaaaat gtcactgtca gccagtcctg cggtaagtgg tacatcagta ttcagacaga
    22621 aagtgaagta tcaactccgg ttcacccttc agcatcaatg gtcgggctgg atgctggcgt
    22681 ggctaaactc gccacgctgt cagatggcac agtctttgag cctgtaaaca gttttcagaa
    22741 aaaccagaag aagctggcga gacttcagcg acagttaagc cgcaaggtca aattcagcaa
    22801 caactggcag aagcagaaac gcaaaataca gcgactgcat ttctgtatcg caaatatccg
    22861 cagggactac cttcacaaag tcacaacgac cgtcagcaaa aaccacgcaa tgatagtcat
    22921 tgaggatttg aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca gccagccggg
    22981 gcgcaatgtc cgggcaaaat caggtttaaa ccgttcgata ctggatccgg gctggtatga
    23041 aatgcgccgc cagcttgagc acaagcagct ctggagtggc ggtcaggtgc ttgctgttcc
    23101 gccagcgtac acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag aaaaccgcct
    23161 gtcacaaagt aaattcagat gccaggtatg tggatataca gcgaacgccg atgtaaatgg
    23221 cgctcgtaac attttagcgg cggggcacgc cgttcttgcc tgtggagaga tggtgcagtc
    23281 aggccgcccg ttgaagcagg aacccaccga aatgattcag gcgacagcct gaacgtagca
    23341 gggatccacg tccttcaggg cgtggaggat gtcaattcat aggcctggta agcgcagcgc
    23401 atcaggccat tacgtcgtaa aatccggcag agccctctct gccggacata ctcattaatg
    23461 aacttgcgga tctgccggag acgcgttgtt gcggatttca gcaatatcca tcgcattgaa
    23521 cagatagtgg ttaccgcagt aatcacaatg catgtcaatt tcgccatctt ccgccaggat
    23581 gctatcgact tcttcatccg gcagcgtttt tagcgcgtcg gcgcaacgtt cacgcgagca
    23641 ggtgcatttg aactccacat cctgcggatc gtaaaccgtc acctcttctt cgtgatacaa
    23701 acgccacaac acttcgtttg ccggtaaggt cagcagttct tcggttttga tggtttcggt
    23761 tagcgtcgcc aggtggtcaa agtcgtcctg ctgggcattt tgcgcaggca ttacctgcaa
    23821 caacataccg cctgcagccg gtttgccgtc tacgtcgccg gtgcgaataa aaaggcgcgt
    23881 cggcagctgt tcagaacgca taaagtaatc ttccaggcag gccgccaggg tatcaccttc
    23941 cagacccacc acgccctgat agcgttcgcc ttcgcttggg gtgatggtga tcaccacgta
    24001 accattgccg accagcgttt tcaggtcggc attttctgga atttcgccct gcacgcgcgc
    24061 cacaccgcgc atctgctggt tgttgttacc gttaataacc gccagattca tcggaccgtc
    24121 gccttgcagc tgtacggtga tatcaccatc aaacttcaac gtagcggtta acaggctggt
    24181 cgcaaccagc agttctgcca gcacgttctt aacgggctgc ggataatcgt gattctcaag
    24241 gatctgttgc agggtttccg aaacggttac cagttcgccg cgaacggcaa agttttcaaa
    24301 cagatagcga tgtaattggt catgttgcgg cataatcatc tctcttgcag gtgacagtta
    24361 ttcactgtcg ccgtgtttaa atcgtaacag gtcgcggcgc tcttttttgt ccgggcgtcg
    24421 gtccggatgc ggcatggtta aggcattaag tttacgtgcc agcgccattt tttcgcgttt
    24481 ctctacactt tccgcagtct cttcatacag caaggctgcc tcgctggcgg ggcgacgctg
    24541 ttcagtaatc gcctttacaa tcaccgtgcg ttcgtcattt ccctggcgca gagtgagcgt
    24601 ggcattcagc tcgacgattt tgcttggctt gctgcgctgc ccgttgtaat gcaccttacc
    24661 gccttcaatc atttcacggg ccagcgcgcg ggttttataa aaacgggcag cccatagcca
    24721 tttatccagt cgaacctcaa cagcaggttt ttctttcatg gcatctcctt cacattagcg
    24781 aggggatcag gcggcggtag tcattcagtg acggatggcg ttgatactgt ttctcggcaa
    24841 tcccggagtc aggattagtc acaccgaggc agtaacgaat accaaattgc gcggcagcat
    24901 cgagaatcgc ttcgctgtca tcaataaaca gcgttctttc agctttaaga cccgtagctt
    24961 cggccaccgc atgccataac cgctgatcct ctttcggata accaaatgtg tgggtggaaa
    25021 gtaataaatc aaggtgtgcg tccagaccgg tatgctcaag ttttaccgcc aggttgtgcg
    25081 gatgcgcatt ggtgagcaaa attcgctgtt taccgctggc tttcagtgcc tcaagaaacg
    25141 gaatggtatc ttcacgcagt acggcacgcg gtcccatctc ggtggtcatc gcacagatat
    25201 ccagacccag ttgctcactc cagtaatcaa gacagtacca gtttagcgta tgctgtacgt
    25261 cgtgatattg ctggcgcata tattccatcg cttcctgtgg cgtaactccg tttttcgcgc
    25321 cccatgtttc aggcaccagc ttttgccaga aatagttatc gaaggcgagg tcgagcaacg
    25381 tgccgtccat atccagcaga acggtatcta cgtcctgcca ggcaatgttg atatgcatga
    25441 gggaaatctc cagagtgaag caatttgcgc gacagggtag cataacctgc cgcgcaaacg
    25501 tgttattcga taaggctttc tgaaggggtg atcagttgcg ggttcaggca gctttcataa
    25561 taggcctgaa tcttcatcat gcgagtacga tggcgctggt aacgacgcca ggcctgtacg
    25621 ccattataaa tggcgctacc cagcatcgtc agtagcagca gcgtggtgct gagatagcgc
    25681 cacaggccgg aacgatccgg gatcggatga aggccaatat gctgcgtacc attggcgtca
    25741 gtgaagattt ttgtgacgat accttcggcg ttaaacggcg tatgcatcag catttgtgcc
    25801 agtttctgga aagcgttcca ctgttcttgc ggcggatagt cgtaaagtga tgccgaaggc
    25861 cagggctgat caacaaaatc gctgccttcg tcgctgacaa tcaggaatcc gccgggggcc
    25921 ggactgttta gtgattgtgc cgctcgcgcc gtttcatgcg tgataaacgg cgcggtagag
    25981 gtggccacca ggttatccag cgattccgca ctcaccgggc gtaataacac attcacgcca
    26041 tcgagtttcc cggcgttggc gcgttttacc agcgcgtccc agtctttact gttgccgaga
    26101 ttgaccagcg catttttcag tcgcacacag tcatctttgg cagaacacag atctgctgtc
    26161 ttcagaacaa tgtcgccaaa atcatcaagc aataccatac cggatttttg aattgctgaa
    26221 cgtaatgagg cgctgacgcg agattcatct tccggtttag ggtgcagctg gcgattaact
    26281 gcttcggtca atgccgtcgc tttgttgacc aattcagatt ctggtaatgg caatgagcgg
    26341 gcgtcattcc agatgatctg cgagcagtca aacggtaaaa aaggtgagtt ggttttcgcg
    26401 ctccaggtac cggaagtgcg aatattacac attcccgtac cgctaatacg caatgtatcg
    26461 ccaacccgca cgccagcgtc agccagttgt ttcacgctgg tggcttcaat ggtctgcgcg
    26521 cctttcatcc acgagagggt gaatttcagc ggcatatcca gcgggatcca gaataacagc
    26581 ataaacagca ccaatagcga gcccgcagca ataatcgtac tgcgcagcca gtgctgtaac
    26641 ggaaagtttt ttacttcatc atgcagcgaa agatatcgcc cctgacgcac tacgtggcgg
    26701 tcgagataga tatcgatatc ggtttgttga ccaagatcct gagcaatgta tggctgccag
    26761 tgtgcgggat agaccaggtc gataataccg agtgaaatat tgttgatctg ttcctgatcg
    26821 ttttcgccaa acaatcccca acgacggggt gtaccgcgca gacaatgaat ttcccgcagg
    26881 gaggattttg ccgggggcgc gaatagcccc cacagacctg cgcccagcag cagtaacgcg
    26941 ccgcctgcca gccacggaac aaagacatcc ggggtaatca ggcagaaaaa gaacatcatg
    27001 aaggaggcga caatcagcaa cgcttcgcgc agcccgcgcg gacgactcag cgcatactct
    27061 tcgtgcgttt ctttgcgaat attgagcagc tctatttgct cgctctcttc cccgcgaatc
    27121 gacgcctgcg ttgacggaac aggttgcaag gcatagccgc gagtttcctg catgtactcc
    27181 tgcagcgtat gaccgttgag tgaaataacc agcggcagcg aatcggtatg aatcagttca
    27241 acggtattct catcgttgat gtactgttcc cagaaagggg gcaggtggac ttctaccgaa
    27301 tcgaggtagt aacgccattt attcggatcg tcggtagaga tgccataacg cgtgatagcg
    27361 tgtgtcagca tcatgacgtt gttgctttcg gcattcagcg ccagagagat cggtgccgcg
    27421 ctggctcccg ttgggccagg cacctgtaat acctgcgtca ggctctcaag ataattttca
    27481 acggcgctac gttcttcaga tgtgagttta cgcgtttgcg catccgcgaa ggcgttggtc
    27541 cagggcagct gacgccgtct ggatcgcact tttatcagcc atcccgcaag tagtgagcag
    27601 gccagcaaag cagctaaaaa aatcacaatg gtgctcatgc tttccccatc ttacttagtc
    27661 tgtcaggcgt ggtcagtcgc accggacaca atacatgatt ctgcggttgg ctatcggcaa
    27721 gcgtggagtt taacttgagc atcattaagc acatcacagt aaccgctgat catagcaaac
    27781 cctctgaagg cggaatatca gcaaattcct ggagttattt caacctattg acttttaatt
    27841 tgatttgagg acgattattt attaagttac ataaatgtaa ataacacgca attcacattg
    27901 ccgaaagcgt gtccgatatc gcacaataac cgctgatttc acagcataga tcccacgatg
    27961 agcaaatcat tacaaaaacc caccattctg aatgttgaaa ctgtagcccg ttcccgactg
    28021 tttaccgtcg aaagcgtgga tctggagttc agcaatggcg tgcggcgtgt ttatgaacga
    28081 atgcgtccaa ccaaccggga agcagtgatg attgtgccga ttgtggacga tcacctgatc
    28141 ctgatccgcg aatacgcagt gggaactgaa tcctacgaat taggtttttc gaaaggatta
    28201 attgatccgg gtgaaagcgt ctttgaagcc gctaaccgcg agctaaaaga agaggttgga
    28261 tttggtgcga acgatctgac ttttttgaag aagctcagca tggcaccgtc ttacttttcc
    28321 agcaaaatga atatcgtggt agcgcaagat ctctacccgg aatcactgga aggcgatgag
    28381 ccagagccgt taccacaggt gcgctggccg ctggcgcata tgatggattt gctggaagcc
    28441 cctgacttca acgaagcgcg taatgtcagc gcgctgttcc tcgtgcgcga atggttgaaa
    28501 gggcaggggc gagtgtaaat gcgtgaagtt tgcctgatgc ataatgctta tcaggcttac
    28561 gaagttcctg caatttattg aattttcaat tttttgttgg ccggataacg cgttcacccg
    28621 catccggtat aaacaaagtg cactttgtca gcaaacttaa aaaggcgctg aagcgccttt
    28681 ttaatcagaa caattcctgt gcctcgccat tatcgataat ggtcgttccc acctcgtgca
    28741 ccgcctgttg tgtcggctgc gtaccttcga tgaaatactc ttcgcggctg ttgccaccat
    28801 tagctaattg cccggtgctg cgatcgatat tcaccgtcac aatacccggt ggcggcgtca
    28861 gcggctgctc cggcacacct tcaagaacgg ctttcatata agcgtcccat gcaggctggg
    28921 cactcttggc accgccttcg taacctgaga tctgatcttt aatcgctccg gaagccgttg
    28981 tatgaccgag attacgacgg tgatcatcaa agccaatcca gaccgaggtc acaacgcccg
    29041 gaccgtaacc cgagaaccac gcatctttcg aactgttagt ggtacccgtt tttccgccta
    29101 tgtcatgccg ttgtaaatca cgacctgctc gccagccagt accctgccag cctggttcac
    29161 caaaaatatt ggtgttcaag gcactcttaa tcaggaatgc cagcggtgtg ttgatgacgt
    29221 gcggtgcgta ctcctgcgcg ccagtcttcg ccactaacgc ctgatttgcc tgctccagct
    29281 gcggcattgg tacagaaaca ttctgctgct cgcgggagat agcgacatct tcaacatcgt
    29341 tattttccag cacgttcgat ttctgcgtat caccgtaaat caccggaata tcgcattccg
    29401 ggcaggctac tttcggtttc gcttcgaaaa tcacgccgcc ctgatcgttt tcaattttgc
    29461 tgataaacca cgggtccacc aggaagccgc cgttcgccat gaccgcgtag ccgcgcgcca
    29521 cctgcattgg ggtgaaggaa gctgaaccca gcgccagcga ttcggtgtgg acaatgtttt
    29581 gtgccgggaa gccgaagcgt tgcagatatt ctgcagcgta gtcgacgccc atcgcccgca
    29641 ttgcgcgtac catcaccacg tttttcgact gacccagccc ctgacgtaag cgaattggac
    29701 cggcatattg cggtggtgag ttcttcggct gccagtcaga accggcacct gcatcccagc
    29761 gggaaattgg cacatcgttc aacatacttg ccagcgttag acctttatcc atcgccgcgg
    29821 tgtagaggaa cggtttgatg ttggaaccca cctgacgcag tgcctgggtg gcgcggttaa
    29881 acttgctctg attgaaatca aagccaccga ccagcgccat aacggcaccg ttttgcggat
    29941 tgatcgacac cagcgccgag ttcacttccg gcacttgcgc cagccaccat gcatcgtcaa
    30001 cttgacgaac ccagatttgc tgacccgttt gcagaacatc ggtcacttta cgcggcgtcg
    30061 gtccctgctg agtatccgaa cggtaaggac gcgcccagcg aacaccttcc atactcaata
    30121 cgacggtcga cccgtctgcc agcatcgccg ttgcttcctg aggattggcg ctggtgactg
    30181 cggcaggcag cagcggacca taggtcggca gcgccttcag cgtatcggta attttgttgt
    30241 tgtcccacgc cgattcgccc actttccaca gcacatttgc cgggccgcga tagccgtggc
    30301 gcatgtcgta gtccagcacg ttattacgta ccgcctgctg tgcggcctgc tgcactttgc
    30361 gggtgatggt ggtgtaaatg cgataaccgt cttcataggc actttcgcca taacggttat
    30421 acatctcctg gcgcaccatt tcgctcaggt atggcgcaga gaaagcaatc tccggcgcgt
    30481 gatagttagc gttaatcgcc tcagtgcgtg tctgatcgaa ctgttgttgg gtgatatatc
    30541 cttcatccag catccgcgac agcacgacgt tacgccgcgc gacggcacga tccattgagt
    30601 agagcgggtt gaaggtggaa ggcgctttcg gcagcccggc tatcaccgcc atttcgttca
    30661 acgtcagttg gtcgaccgtt ttaccgaaat agacttgtgc cgcagcaccg acaccatagg
    30721 cgcggtaacc gaggtaaatc ttgttcagat aaagctcgag gatctcgtct ttcgtcagca
    30781 actgttcaat gcgaatcgcg aggaagactt ccttaatctt gcgcatcagc gtgcgttctg
    30841 gactgaggaa gaagtttctc gccagctgct gggtaatagt actcgcccct tgtgacgcgt
    30901 gaccggagaa cagcgccacg cttgccgcac ggaagatccc caccgggtca acgccgtgat
    30961 gctcgtagaa gcggctatct tccgtcgcga taaaggcttt caccatctcc ggcgggattt
    31021 gatccaacgt aaccggaata cgacgtttct caccgtattg agcaatcagc tcgccatcgg
    31081 cgctgtaaat ctgcatcgga atttgcaggc gaacatcttt taatgtcgcc acatccggca
    31141 gttgtggctc gatgtagcgg tataggccat aaatcgagcc tgctcccagc agaatgcaac
    31201 agactgcaag gatcaaaaaa tactttacga acttcactgg aaatttccca tttagtttca
    31261 tttgggcagt ttataaacaa acgcgcggta gtataaaggc aagccagacg cattgatata
    31321 cctgtcagag tgacgggtga taaggagatc atcacaatgg catttaagaa ctggcaaatt
    31381 ggtttgcatt tacaacagca agaagcggta gcggttgcta tcgtgcggag cgcaaaagaa
    31441 tgcttattgc accgctggtg gcggttgcca ctggagaacg acattatcaa agatgggcgg
    31501 attgttgatg tgcagcggct ggctaacacg ttgttaccgt ggagtcgtga actgccgcag
    31561 cgtcatcaca ttatgttggc gtttcccgcc agtcgcacat tacagcggtc atttccacgc
    31621 ccgtcgatgt ccctcggtga gcgggagcaa atggcctggc tgtccgggac gatggcccgt
    31681 gaactggata tggatccgga ctccctgcgc ttcgattaca gcgaagactc actcagtccc
    31741 gcctataacg tgactgccgc gcaaagcaaa gagctggcaa cgctgcttac tctggcagaa
    31801 aggttgcgtg ttcatgttag cgcgatcact ccggatgcca gtgcattaca gcgattcctg
    31861 ccttttttac cttctcatca gcaatgtctg gcctggcgtg ataacgaaca gtggctgtgg
    31921 gcgacgcgct atagctgggg gcgcaaactg gcggtgggga tgactagcgc gaaggagctg
    31981 gcggcagcgt tatctgttga tcccgaaagc gtcgcgctat gtggcgaagg cggatttgac
    32041 ccctgggagg cggtttctgt tcgtcagccg ccgctaccgc caccaggtgg agactttgcc
    32101 atcgcgctgg ggctggcgct tgggaaggtg tactgatgaa cccgccaatt aattttttgc
    32161 cctggcgaca gcaacgccgg actgcttttc ttcgtttctg gttgctgatg ttcgttgcgc
    32221 ctctgctgct ggccgtcggg ataacgctaa tactgcgtct gacaagcaat gccgaagcac
    32281 gcgtaaacgc cgttttgctt caggcggaac aacaactcgc ccgcagctta cagataacga
    32341 aaccacgttt gctggaacgc caacagttac gcgaacagcg tttgcaaagg cagcgccagc
    32401 gacaatttac ccgcgactgg caatctgcgc tggaagcact ggcggcgctt ttacccgaac
    32461 atgcctggct gacaacgata agctggcagc agggaacgct ggagatcaag gggctgacaa
    32521 caagcattgc cgcgttaaac gcgctggaaa cgtcactccg ccaggatgct tcttttcatc
    32581 tcaatcagag gggggccacg cagcaggatg cgcagggacg ctggcaattt gagtatcagt
    32641 taacaaggaa ggttagcgat gaacatgttc tttgactggt ggttcgccac atcactccgc
    32701 ctccgccagt tttgctgggc agtctggttg ctggtgttag ttacgctcat ttttctgtca
    32761 ttgacacacc atgaagagag cgatgcatta attcgactac gggcaaatca tcaccagcag
    32821 tgggctgcac tgtatcgcct ggtagacacc acttccttca gcgaagaaaa aacgctgccc
    32881 ttttcgccac tggattttca gttatccggc gcgcaactgg tttactggca tccatccgcg
    32941 cagggaggtg agttggcgtt gaaaacgctg tgggaagcag tgccgtcggc atttacacgg
    33001 ctggcagagc gcaacgtcag cgtgagccgt ttttcgttaa gcgtggaagg tgatgacctt
    33061 ttgttcacgc tacaactgga gacgccgcat gagggttaaa cgctggttgt tggcaggtat
    33121 tgcattgtgc cttttaaccg gtatgcgtga cccttttaaa ccgccggaag atctatgccg
    33181 gattagtgaa cttagccagt ggcgctatca gggaatggta gggcgaggcg agcgcatcat
    33241 cggtgtaata aaagatgggc aaaaaaaatg gcgacgggtg cagcaaaacg atgtgctgga
    33301 aaacggctgg acgatcttac agctgacgcc agacgcacta acgctgggaa ccgggacaaa
    33361 ctgcgaaccg ccacactggt tgtggcaacg gcaaggagat acaaatgaag caatggatag
    33421 ccgcactact gttgatgctt atacccggcg tacaggcggc aaagccgcaa aaagtgacgc
    33481 tgatggtgga tgacgttccg gtagctcagg tgttgcaggc gctggctgaa caggagaagt
    33541 tgaacctggt ggtttcgcca gacgtcagcg gtacggtgtc gttacattta actgacgttc
    33601 cctggaagca ggcactacaa actgtagtga aaagcgccgg attgataacg cgccaggagg
    33661 gcaacattct ctcggtgcat tccgttgcct ggcagaatga caatatcgcc cgccaggagg
    33721 cggagcagac gcgggcgcag gcaaatctgc cgctggaaaa tcgcaatatt actctgcaat
    33781 acgccgacgc cggagagctg gcgaaagcgg gggagaagct actgagtgcc aaagggagta
    33841 tgaccgtcga taaacgcacc aatcgccttt tgctgcgaga taacaaaacg gcgttaagca
    33901 cgcttgaaca gtgggtatcg caaatggatc tgccggtcgg gcaggttgag ctctcggcgc
    33961 atattgtcac cattaatgaa aaaagtttgc gtgagttagg tgtgaaatgg acgctggccg
    34021 atgcgcaaca agctggtggc gttgggcaag tcaccacgct tggcagcgac ctctccgtag
    34081 cgacggcgac aacgcatatc ggttttaaca ttggacgcat caacggacgt ttactggatc
    34141 ttgagctttc cgcgctcgaa caaaaacagc agctggatat tatcgccagt ccgcgtctgc
    34201 tggcctcaca tcttcagcct gccagcatta aacaggggag cgaaattcca tatcaggttt
    34261 ccagcgggga aagtggcgcg acgtcggtgg aatttaaaga ggccgtcctg gggatggaag
    34321 tcacgcccac ggtgttacaa aaaggtcgta tccggctgaa attacacatc agccagaacg
    34381 ttccggggca ggtgctacag caggcagatg gcgaagtgct ggcgattgat aagcaggaga
    34441 tcgaaacgca ggtcgaggtc aaaagcggag aaacgttggc gctgggcggc atttttaccc
    34501 gtaaaaataa atcgggtcag gatagcgtac cgttgcttgg cgacattccc tggttcgggc
    34561 aattatttcg tcatgacgga aaagaagatg aacgacgcga gttagtggtg tttatcacgc
    34621 cacgactggt ttccagtgag taaacagccg taaaagcggt aatgttttta cgctgaacgt
    34681 gtttcatcta tttgacgcgc gcaggtattt agcatacaag gagtaccgat ttgagagttg
    34741 gtgctcttcg ctgcctgcgt tccatgatga tgatttatca ttcaggcggc attttgctgt
    34801 cttttttacg ctaatcttac ccggtgattt atcgccagag cggtggtagc aaggcagcgc
    34861 gcttgcagcg accagatatg cagagggatg ggtgatttat tcagttgcca aacccgctgg
    34921 agtattgaga taattttcag tctgactctc gcaatatctt atgaggtttc agttcatgtc
    34981 ctgcggcgct ctctgagcga agcgggttta tcattaacga atagtcttag tagtaccgaa
    35041 aaaatggcag agaaacgcaa tatctttctg gttgggccta tgggtgccgg aaaaagcact
    35101 attgggcgcc agttagctca acaactcaat atggaatttt acgattccga tcaagagatt
    35161 gagaaacgaa ccggagctga tgtgggctgg gttttcgatt tagaaggcga agaaggcttc
    35221 cgcgatcgcg aagaaaaggt catcaatgag ttgaccgaga aacagggtat tgtgctggct
    35281 actggcggcg gctctgtgaa atcccgtgaa acgcgtaacc gtctttccgc tcgtggcgtt
    35341 gtcgtttatc ttgaaacgac catcgaaaag caacttgcac gcacgcagcg tgataaaaaa
    35401 cgcccgttgc tgcacgttga aacaccgccg cgtgaagttc tggaagcgtt ggccaatgaa
    35461 cgcaatccgc tgtatgaaga gattgccgac gtgaccattc gtactgatga tcaaagcgct
    35521 aaagtggttg caaaccagat tattcacatg ctggaaagca actaattctg gctttatata
    35581 cactcgtctg cgggtacagt aattaaggtg gatgtcgcgt tatggagagg attgtcgtta
    35641 ctctcgggga acgtagttac ccaattacca tcgcatctgg tttgtttaat gaaccagctt
    35701 cattcttacc gctgaaatcg ggcgagcagg tcatgttggt caccaacgaa accctggctc
    35761 ctctgtatct cgataaggtc cgcggcgtac ttgaacaggc gggtgttaac gtcgatagcg
    35821 ttatcctccc tgacggcgag cagtataaaa gcctggctgt actcgatacc gtctttacgg
    35881 cattgttaca aaagccgcat ggtcgcgata ctactctggt ggcgcttggc ggcggcgtag
    35941 tgggcgatct gaccggcttc gcggcggcga gttatcagcg cggtgtacgt ttcattcaag
    36001 tcccgacgac gttactgtcg caggtcgatt cctccgttgg cggcaaaact gcggtcaacc
    36061 atcccctcgg taaaaacatg attggcgcgt tctaccagcc cgcttcagtg gtggtggatc
    36121 tcgactgtct gaaaacgctt cccccgcgtg agttagcgtc ggggctggca gaagtcatca
    36181 aatacggcat tattcttgac ggtgcgtttt ttaactggct ggaagagaat ctggatgcgt
    36241 tgttgtgtct ggacggtccg gcaatggcgt actgtattcg ccgttgttgt gagctgaaag
    36301 cagaagttgt cgccgccgac gagcgcgaaa ccgggttacg tgctttactg aatctgggac
    36361 acacctttgg tcatgccatt gaagctgaaa tggggtatgg caattggtta catggtgaag
    36421 cggtcgctgc gggtatggtg atggcggcgc ggacgtcgga acgtctcggg cagtttagtt
    36481 ctgccgaaac gcagcgtatt ataaccctgc tcacgcgggc tgggttaccg gtcaatgggc
    36541 cgcgcgaaat gtccgcgcag gcgtatttac cgcatatgct gcgtgacaag aaagtccttg
    36601 cgggagagat gcgcttaatt cttccgttgg caattggtaa gagtgaagtt cgcagcggcg
    36661 tttcgcacga gcttgttctt aacgccattg ccgattgtca atcagcgtaa caacaagaaa
    36721 ggtcaggccg cttatcaagc ggtctattag cttcaggtta attgcaacgt ggtaagcatt
    36781 aaccttttag tggggtgtta aatggatgaa ttcaaaccag aagacgagct gaaacccgat
    36841 cccagcgatc gtcgtactgg tcgttctcgt caatcttctg aacgttctga gcgtactgaa
    36901 cgtggcgaac cgcagatcaa ttttgatgat attgaacttg atgacactga cgatcgccgt
    36961 ccgactcgtg cgcaaaaagc gcgtaatgag gaaccggaaa tcgaagaaga aattgacgaa
    37021 tccgaagatg aaaccgtgga tgaagagcgc gtagagcgtc gtccgcgtaa gcgcaaaaaa
    37081 gcagccagta aacccgcttc tcgtcagtat atgatgatgg gtgtcggcat tctggttcta
    37141 ctgctgttga tcatcggtat cggttctgcg ctaaaagccc cctcgacctc ttccagcgat
    37201 caaaccgcgt ctggcgagaa gagtattgat cttgcaggca atgcgaccga tcaggccaat
    37261 ggcgtgcagc cagcgccggg aaccacgtct gcggaaaata ctcagcagga tgtttctctg
    37321 ccgccgatct cttctacgcc gactcaaggg caaaccccgg cggcaacgga tggtcagcaa
    37381 cgtgttgaag tgcagggtga cctgaacaat gcgctgaccc agccacaaaa tcagcaacag
    37441 ttgaacaatg tggcggtcaa ttctacattg ccgactgaac ccgcaacggt cgcgcctgtt
    37501 cgcaatggca atgcatcgcg tgacacggcg aaaatgcaaa ccgctgaacg tccggccact
    37561 acgcgtccag ctcgccagca ggcggtgatt gaaccgaaaa aaccgcaagc aaccgtgaaa
    37621 acggagccga agccggtagc acagacgccg aagcgtactg aaccagctgc tcctgtggca
    37681 agcacgaagg caccggctgc gacctctacg ccagcaccaa aagagaccgc aactacggct
    37741 ccagtacaga cggcatcccc ggcgcaaacc acggcaacac cagccgctgg agggaagacc
    37801 gcaggtaatg tgggttcgtt gaaatcggca ccgtccagcc attacactct gcagctgagc
    37861 agttcctcta actacgacaa cctgaacggt tgggcgaaga aagagaatct gaaaaactac
    37921 gttgtctatg aaacgacgcg taatggtcag ccgtggtatg tcctggtttc tggcgtgtat
    37981 gcttcgaaag aagaggcgaa aaaagcggta tctacattgc cagcagatgt ccaggccaaa
    38041 aacccgtggg cgaaaccgct gcgtcaggta caggccgatc tgaagtaatc aaggttatct
    38101 cccgcaatgg tttatcattg cgggagttgc ctgaagcgct ggatgctgtc ggagctttct
    38161 ccacagccgg agaaggtgta attagttagt cagcatgaag aaaaatcgcg cttttttgaa
    38221 gtgggcaggg ggcaagtatc ccctgcttga tgatattaaa cggcatttgc ccaagggcga
    38281 atgtctggtt gagccttttg taggtgccgg gtcggtgttt ctcaacaccg acttttctcg
    38341 ttatatcctt gccgatatca atagcgacct gatcagtctc tataacattg tgaagatgcg
    38401 tactgatgag tacgtacagg ccgcacgcga gctgtttgtt cccgaaacaa attgcgccga
    38461 ggtttactat cagttccgcg aagagttcaa caaaagccag gatccgttcc gtcgggcggt
    38521 actgttttta tatttgaacc gctacggtta caacggcctg tgtcgttaca atctgcgcgg
    38581 tgagtttaac gtgccgtttg gccgctacaa aaaaccctat ttcccggaag cagagttgta
    38641 tcacttcgct gaaaaagcgc agaatgcctt tttctattgt gagtcttacg ccgatagcat
    38701 ggcgcgcgca gatgatgcat ctgtcgtcta ttgcgatccg ccttatgcgc cgctgtctgc
    38761 gactgccaac tttacggcgt atcacacaaa cagttttacg cttgaacaac aagcgcatct
    38821 ggcggagatc gctgaaggtc tggttgatcg ccatattcca gtgctgatct ccaatcacga
    38881 tacgatgtta acgcgtgagt ggtatcagcg cgcaaaattg catgtcgtca aagttcgacg
    38941 cagtataagc agcaacggcg gcacacgtaa aaaggtggac gaactgctgg ctttgtacaa
    39001 accaggagtc gtttcacccg cgaaaaaata attctcaagg agaagcggat gaaacagtat
    39061 ttgattgccc cctcaattct gtcggctgat tttgcccgcc tgggtgaaga taccgcaaaa
    39121 gccctggctg ctggcgctga tgtcgtgcat tttgacgtca tggataacca ctatgttccc
    39181 aatctgacga ttgggccaat ggtgctgaaa tccttgcgta actacggcat taccgcccct
    39241 atcgacgtgc atctgatggt gaaacccgtc gatcgcattg tgcctgattt cgctgccgct
    39301 ggtgccagca tcattacctt tcatccagaa gcctccgagc atgttgaccg cacgctgcaa
    39361 ctgattaaag aaaatggctg taaagcgggt ttggtattta acccggcgac acctctgagt
    39421 tatctggatt acgtgatgga taagctggat gtgatcctgc tgatgtccgt caatcctggt
    39481 ttcggcggtc agtctttcat tcctcaaaca ctggataaac tgcgcgaagt acgtcgtcgt
    39541 atcgacgagt ctggctttga cattcgactg gaagtggacg gtggcgtgaa ggtgaacaac
    39601 attggcgaaa tcgctgcggc gggcgcggat atgtttgttg ctggttcggc aatcttcgac
    39661 cagccagact acaaaaaagt cattgatgaa atgcgcagtg aactggcaaa ggtaagtcat
    39721 gaataagttt gaagatattc gcggcgtcgc ttttgatctc gacggtacgc tggttgacag
    39781 tgcacccggt cttgctgctg cggtagatat ggcgctgtat gcgctggagt tgcctgtcgc
    39841 aggtgaagaa cgcgttatta cctggattgg taacggcgca gatattctga tggagcgcgc
    39901 attgacctgg gcgcgtcagg aacgtgcgac tctgcgtaaa acaatgggta aaccgcccgt
    39961 tgatgacgac attccggcag aagaacaggt acgtatcctg cgtaaactgt tcgatcgcta
    40021 ctatggcgag gttgccgaag aggggacgtt tttgttcccg cacgttgccg atacgttggg
    40081 cgcgttgcag gctaaaggcc tgccgctagg cctggtcacc aacaaaccga cgccgttcgt
    40141 cgcgccgctg ctcgaagcct tagatatcgc caaatacttc agcgtggtga ttggtggtga
    40201 tgatgtgcaa aacaaaaaac cgcatccgga cccgctgtta ctggtggctg aacggatggg
    40261 cattgctcca caacagatgc tgtttgttgg cgactcacgc aatgatattc aggcagcaaa
    40321 agcggcaggt tgcccatcag ttggcttaac ctacggatat aactacggcg aggctatcga
    40381 tctcagccag cctgatgtaa tttatcagtc tataaatgac cttctgcccg cattagggct
    40441 tccgcatagc gaaaatcagg aatcgaaaaa tgactaagcc catcgttttt agtggcgcac
    40501 agccctcagg tgaattgacc attggtaact acatgggtgc gctgcgtcag tgggtaaaca
    40561 tgcaggatga ctaccattgc atttactgta tcgttgacca acacgcgatc accgtgcgcc
    40621 aggatgcaca gaagctgcgt aaagcgacgc tggatacgct ggccttgtat ctggcttgtg
    40681 gtatcgatcc tgagaaaagc accatttttg ttcagtccca cgtgccggaa catgcgcagt
    40741 taggctgggc actgaactgc tatacctact tcggcgaact gagccgcatg acgcagttta
    40801 aagataaatc tgcgcgttat gccgagaaca tcaatgctgg tctgtttgac tatccggtgc
    40861 tgatggctgc ggacatcctg ctgtatcaaa ctaatctggt accggtgggt gaagaccaga
    40921 aacagcacct ggaactgagc cgcgatatcg cccagcgttt caacgcgctg tacggcgata
    40981 tctttaaagt gccggagccg tttattccga aatccggcgc gcgcgtaatg tcgctgctgg
    41041 agccgaccaa gaagatgtcc aagtctgacg ataaccgcaa taacgttatc ggcctgctgg
    41101 aagatccgaa atcggtagtg aagaaaatca aacgcgcggt cactgactcc gacgagccgc
    41161 cggtagttcg ctacgatgtg cagaacaaag cgggcgtttc caacctgctg gatatccttt
    41221 ctgcggtaac gggccagagc atcccggaac tggaaaaaca gttcgaaggc aagatgtatg
    41281 gtcatctgaa aggtgaagtg gctgatgccg tttccggtat gctgactgaa ttgcaggaac
    41341 gttaccaccg tttccgcaac gatgaagcct tcctgcaaca ggtgatgaaa gacggcgcag
    41401 aaaaagccag cgcacacgct tcccgtacgc taaaagcggt gtacgaagcg attggatttg
    41461 tggcgaagcc gtaatttaac attaatgcca aaaaccgggg aaacccggtt tttttatcct
    41521 ctttttgcaa taatccgaaa aaatgtgaag cgcctcgccg tttccacatc tcgc
//
LOCUS       DAZKHK010000029        41194 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKHK010000029 DAZKHK010000000
VERSION     DAZKHK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.029"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            293..1192
                     /gene="lysO"
                     /locus_tag="ACHOS5_001566"
     CDS             293..1192
                     /gene="lysO"
                     /locus_tag="ACHOS5_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP9776390.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNFAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1336..2988
                     /gene="hcp"
                     /locus_tag="ACHOS5_001567"
                     /gene_synonym="priS"
     CDS             1336..2988
                     /gene="hcp"
                     /locus_tag="ACHOS5_001567"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP9776391.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3000..3968
                     /gene="hcr"
                     /locus_tag="ACHOS5_001568"
     CDS             3000..3968
                     /gene="hcr"
                     /locus_tag="ACHOS5_001568"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP9776392.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKTLG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4101..5819
                     /gene="poxB"
                     /locus_tag="ACHOS5_001569"
     CDS             4101..5819
                     /gene="poxB"
                     /locus_tag="ACHOS5_001569"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP9776393.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERNFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGTQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELYDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5856..6857
                     /gene="ltaE"
                     /locus_tag="ACHOS5_001570"
     CDS             5856..6857
                     /gene="ltaE"
                     /locus_tag="ACHOS5_001570"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP9776394.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAVADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWHAFLAR"
     gene            6868..8298
                     /gene="ybjT"
                     /locus_tag="ACHOS5_001571"
     CDS             6868..8298
                     /gene="ybjT"
                     /locus_tag="ACHOS5_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP9776395.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRIAKLQL
                     ANVSCHKVDLNWPDNLPALLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLQALWRVVNQIGGKERYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            8397..9410
                     /gene="ybjS"
                     /locus_tag="ACHOS5_001572"
     CDS             8397..9410
                     /gene="ybjS"
                     /locus_tag="ACHOS5_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9776396.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9407..10237)
                     /gene="amiD"
                     /locus_tag="ACHOS5_001573"
     CDS             complement(9407..10237)
                     /gene="amiD"
                     /locus_tag="ACHOS5_001573"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP9776397.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10234..10557)
                     /gene="ybjQ"
                     /locus_tag="ACHOS5_001574"
     CDS             complement(10234..10557)
                     /gene="ybjQ"
                     /locus_tag="ACHOS5_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9776398.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            10683..11198
                     /gene="ybjP"
                     /locus_tag="ACHOS5_001575"
     CDS             10683..11198
                     /gene="ybjP"
                     /locus_tag="ACHOS5_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9776399.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRGNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11416..12144
                     /gene="artP"
                     /locus_tag="ACHOS5_001576"
     CDS             11416..12144
                     /gene="artP"
                     /locus_tag="ACHOS5_001576"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP9776400.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            12162..12893
                     /gene="artI"
                     /locus_tag="ACHOS5_001577"
     CDS             12162..12893
                     /gene="artI"
                     /locus_tag="ACHOS5_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP9776401.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRIEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            12900..13616
                     /gene="artQ"
                     /locus_tag="ACHOS5_001578"
     CDS             12900..13616
                     /gene="artQ"
                     /locus_tag="ACHOS5_001578"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP9776402.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            13616..14284
                     /gene="artM"
                     /locus_tag="ACHOS5_001579"
     CDS             13616..14284
                     /gene="artM"
                     /locus_tag="ACHOS5_001579"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP9776403.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            14510..15241
                     /gene="artJ"
                     /locus_tag="ACHOS5_001580"
     CDS             14510..15241
                     /gene="artJ"
                     /locus_tag="ACHOS5_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP9776404.1"
                     /translation="MKKLVLAALLTSFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNTALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(15270..16397)
                     /gene="rlmC"
                     /locus_tag="ACHOS5_001581"
     CDS             complement(15270..16397)
                     /gene="rlmC"
                     /locus_tag="ACHOS5_001581"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP9776405.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTVDLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(16438..16926)
                     /gene="ybjO"
                     /locus_tag="ACHOS5_001582"
     CDS             complement(16438..16926)
                     /gene="ybjO"
                     /locus_tag="ACHOS5_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP9776406.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVHGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(16986..17831)
                     /gene="potI"
                     /locus_tag="ACHOS5_001583"
     CDS             complement(16986..17831)
                     /gene="potI"
                     /locus_tag="ACHOS5_001583"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP9776407.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVALSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(17828..18772)
                     /gene="potH"
                     /locus_tag="ACHOS5_001584"
     CDS             complement(17828..18772)
                     /gene="potH"
                     /locus_tag="ACHOS5_001584"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP9776408.1"
                     /translation="MSTLEPAAQPKPFKLWLSQLQMKHGRKLVIALPYIWLILLFLLP
                     FLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQSLQV
                     AAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGV
                     LNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAALDL
                     GARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFF
                     NNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSVGEHG"
     gene            complement(18782..19915)
                     /gene="potG"
                     /locus_tag="ACHOS5_001585"
     CDS             complement(18782..19915)
                     /gene="potG"
                     /locus_tag="ACHOS5_001585"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP9776409.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(20010..21122)
                     /gene="potF"
                     /locus_tag="ACHOS5_001586"
     CDS             complement(20010..21122)
                     /gene="potF"
                     /locus_tag="ACHOS5_001586"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP9776410.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(21472..21948)
                     /gene="ybjN"
                     /locus_tag="ACHOS5_001587"
     CDS             complement(21472..21948)
                     /gene="ybjN"
                     /locus_tag="ACHOS5_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP9776411.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(22036..22938)
                     /gene="rimK"
                     /locus_tag="ACHOS5_001588"
     CDS             complement(22036..22938)
                     /gene="rimK"
                     /locus_tag="ACHOS5_001588"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP9776412.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(22999..23721)
                     /gene="nfsA"
                     /locus_tag="ACHOS5_001589"
     CDS             complement(22999..23721)
                     /gene="nfsA"
                     /locus_tag="ACHOS5_001589"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP9776413.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAQYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(23705..23992)
                     /gene="ybjC"
                     /locus_tag="ACHOS5_001590"
     CDS             complement(23705..23992)
                     /gene="ybjC"
                     /locus_tag="ACHOS5_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP9776414.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            24152..24409
                     /gene="grxA"
                     /locus_tag="ACHOS5_001591"
     CDS             24152..24409
                     /gene="grxA"
                     /locus_tag="ACHOS5_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP9776415.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWAKENLDA"
     gene            complement(24439..24816)
                     /gene="ybjM"
                     /locus_tag="ACHOS5_001592"
     CDS             complement(24439..24816)
                     /gene="ybjM"
                     /locus_tag="ACHOS5_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP9776416.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            25086..26771
                     /gene="ybjL"
                     /locus_tag="ACHOS5_001593"
     CDS             25086..26771
                     /gene="ybjL"
                     /locus_tag="ACHOS5_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP9776417.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(26946..27482)
                     /gene="rcdA"
                     /locus_tag="ACHOS5_001594"
     CDS             complement(26946..27482)
                     /gene="rcdA"
                     /locus_tag="ACHOS5_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP9776418.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVRDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            27566..28774
                     /gene="ybjJ"
                     /locus_tag="ACHOS5_001595"
     CDS             27566..28774
                     /gene="ybjJ"
                     /locus_tag="ACHOS5_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776419.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            28774..29589
                     /gene="ybjI"
                     /locus_tag="ACHOS5_001596"
     CDS             28774..29589
                     /gene="ybjI"
                     /locus_tag="ACHOS5_001596"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP9776420.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ"
     gene            29675..29959
                     /gene="ybjH"
                     /locus_tag="ACHOS5_001597"
     CDS             29675..29959
                     /gene="ybjH"
                     /locus_tag="ACHOS5_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP9776421.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(30000..31232)
                     /gene="mdfA"
                     /locus_tag="ACHOS5_001598"
     CDS             complement(30000..31232)
                     /gene="mdfA"
                     /locus_tag="ACHOS5_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP9776422.1"
                     /translation="MQNKLASGVRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            31517..32113
                     /gene="ybjG"
                     /locus_tag="ACHOS5_001599"
     CDS             31517..32113
                     /gene="ybjG"
                     /locus_tag="ACHOS5_001599"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9776423.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            32171..32929
                     /gene="deoR"
                     /locus_tag="ACHOS5_001600"
     CDS             32171..32929
                     /gene="deoR"
                     /locus_tag="ACHOS5_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP9776424.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLIEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(32976..34178)
                     /gene="dacC"
                     /locus_tag="ACHOS5_001601"
     CDS             complement(32976..34178)
                     /gene="dacC"
                     /locus_tag="ACHOS5_001601"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9776425.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLMEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            34425..35051
                     /gene="gstB"
                     /locus_tag="ACHOS5_001602"
     CDS             34425..35051
                     /gene="gstB"
                     /locus_tag="ACHOS5_001602"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP9776426.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(35048..36163)
                     /gene="yliI"
                     /locus_tag="ACHOS5_001603"
     CDS             complement(35048..36163)
                     /gene="yliI"
                     /locus_tag="ACHOS5_001603"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP9776427.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(36274..36657)
                     /gene="bssR"
                     /locus_tag="ACHOS5_001604"
     CDS             complement(36274..36657)
                     /gene="bssR"
                     /locus_tag="ACHOS5_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP9776428.1"
                     /translation="MFVDRQRIDMLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            36870..38195
                     /gene="rimO"
                     /locus_tag="ACHOS5_001605"
     CDS             36870..38195
                     /gene="rimO"
                     /locus_tag="ACHOS5_001605"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP9776429.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(38242..39570)
                     /gene="dgcI"
                     /locus_tag="ACHOS5_001606"
     CDS             complement(38242..39570)
                     /gene="dgcI"
                     /locus_tag="ACHOS5_001606"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9776430.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(39578..>41194)
                     /locus_tag="ACHOS5_001607"
     CDS             complement(39578..>41194)
                     /locus_tag="ACHOS5_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFP9776431.1"
                     /translation="YNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFY
                     LPFAFIIIVLLVVYLMTTRVFRRQFSEMTDLVNTLAFLPDSTDQIEALKIREGDAKEI
                     ISIKNSIAEMKDAEIERANKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGL
                     EAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAT
                     VNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRNHMFSEFIFCDDAK
                     FNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRTILGAEALCRWVSAERGIISPL
                     KFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHE
                     RFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRVSIDDFGTGLSNL
                     KRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELI
                     KLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT        10141 a        10726 c        10218 g        10109 t
ORIGIN      
        1 cttatccctt acggctaccg ggtcgggcgc gaggtaacat ccctgtaaaa cgcgccctca
       61 gcccacatcc atgtgggctg ccccggcctt cagggaacgc ctcggcgatt ttgacgccac
      121 caacactggt gcggtttgta attaaagcgc aagaacatct ttaaatgaaa acgaatactc
      181 tactgctcct tatgcataac atttcctttt aattgcaact ttactcgtcc ttccgctttc
      241 agtagtgtgt catcgcgcat ttcgacttca tctttgcaag gactgggttt tcatgttttc
      301 tgggctgtta atcattctgg ttcccctgat tgtgggttac ctcattccgc ttcgccaaaa
      361 agctgcatta agagttatta atcagctatt aagctggatg gtttacctta ttctcttttt
      421 tatgggtatc agtctggcgt ttctcgataa cctcgccagt aacctgttgg cgattctgca
      481 ttattctgcc gtcagtatta ccgttatttt actgtgtaat tttgccgccc tgatgtggct
      541 ggagcgcggc ctgccatggc gcaaccacca tcagcaagaa aaactcccgt cgcgtattgc
      601 gatggcgctg gagtcgctaa aactgtgcgg cgtagtagtg attggttttg ccattggtct
      661 gagtggactg gctttcttac aacacgcgac cgaagccagt gaatacacct taattctgct
      721 acttttcctc gtcggtattc agttgcgcaa taatggcatg accttaaagc agattgtcct
      781 taatcgccgg ggaatgattg tcgccgtggt ggtggttgcc agttcattaa ttggtggttt
      841 aattaacgcc tttattcttg atcttcccat caataccgcg ctggcaatgg cctccggttt
      901 cggctggtat tctctttccg gtattttatt gaccgaatct tttggtccgg taatcgggag
      961 cgcggcgttt tttaatgatc tggcccgtga gctgattgct attatgctaa tccctgggct
     1021 gattcgccgt agccgctcta ctgcactggg cttatgcggt gcgacgtcga tggatttcac
     1081 cctgcccgtt cttcaacgta caggcgggct ggatatggtc ccggcggcga ttgttcacgg
     1141 ttttattctt agtctgttag tgccgattct catcgccttt ttctctgcat aatacctctc
     1201 tggcggtaga tctctgccgc caaaattgcg ctaaatcaat ctcccttaaa gttgcattaa
     1261 aaatctcttt tatccccgcg ttaagcgtct taaccttaaa catgtatatt aaatataact
     1321 ttaaagggtg tgatcatgtt ttgtgtgcaa tgtgaacaaa ctatccgtac tccggcagga
     1381 aacggctgct catacgcgca ggggatgtgt ggtaaaacgg cggaaacctc tgaccttcag
     1441 gatttactca tcgcggcgct gcaagggctt tcggcctggg cagtgaaagc acgtgaatac
     1501 ggcatcatca accacgatgt agacagcttt gcgccacgtg cctttttctc aaccctgacc
     1561 aacgttaact tcgattctcc gcgtattgtc ggctacgctc gtgaagcgat tgccctgcgc
     1621 gaggcgctga aagcacaatg cctggctgta gatgccaacg cccgcgtcga taacccgatg
     1681 gctgacctgc aactggtgag cgacgatctc ggcgagctgc aacgtcaggc agcagaattt
     1741 acccctaaca aagataaagc ggcgattggc gaaaacattc tcggcctgcg tctactgtgc
     1801 ctgtatggcc tgaaaggtgc ggcggcctat atggaacacg cgcatgttct cggtcaatac
     1861 gacaacgata tttatgccca gtaccataaa atcatggcgt ggctggggac ctggcctgcc
     1921 gatatgaacg cgcttcttga gtgttcaatg gaaatcggcc agatgaactt caaagtgatg
     1981 agcattctgg acgcaggcga aaccggtaaa tacggtcacc cgaccccaac ccaggtcaat
     2041 gtcaaagcga cggcgggtaa atgcattctg atttccggtc acgatctcaa agatctctac
     2101 aacctgctgg aacagaccga aggcacgggc gttaatgtct acacccacgg cgaaatgctg
     2161 cctgcgcatg gttacccgga gctgcgtaaa ttcaagcatc tggtcggtaa ctacggcagc
     2221 ggctggcaga atcagcaagt ggagttcgct cgtttcccag gccccatcgt gatgacctca
     2281 aactgcatca tcgacccaac cgtaggcgct tatgacgatc gtatctggac ccgcagcatc
     2341 gttggctggc cgggtgtccg tcatctggat ggcgaagatt tctctgcggt cattgcccag
     2401 gcgcaacaga tggcgggctt cccgtacagc gaaattccgc acctgatcac cgtgggcttt
     2461 ggtcgccaga cactgcttgg cgcagccgat acgctgattg atctggtgag ccgtgaaaaa
     2521 ctgcgtcata tcttcctgct tggcggctgt gacggcgcac gcggcgagcg tcactatttc
     2581 accgatttcg ccaccagcgt gccggatgac tgcctgatcc tgaccctcgc ttgcggtaaa
     2641 tatcgcttta ataaacttga gtttggcgat atcgaaggtc tgccgcgtct ggtagatgct
     2701 ggtcaatgta acgatgctta ctcagcaatt attctggcgg taacgctggc agagaaactg
     2761 ggctgcggag tcaacgatct gccgctgtcg ctggtgctct cctggtttga acagaaagcg
     2821 attgtcattc tgctgacgct gctttctctg ggtgtgaaaa atatcgtcac cggcccgact
     2881 gcacctggtt tcctgacacc agacctgctg gcggtgctga acgagaaatt cggcctgcgt
     2941 tctatcacca ctgttgaaga agacatgaag caactgttga gcgcgtaagg agtttgtcga
     3001 tgacgatgcc aacgaatcaa tgcccgtggc ggatgcaggt tcatcacatt acgcaagaaa
     3061 cgccggatgt gtggacgatt tccctgattt gccacgatta ctacccatat cgcgccgggc
     3121 aatatgcgct ggtcagcgtg cgaaactcag cggaaacgct gcgtgcttac accatttcct
     3181 ccacgccagg cgtgagtgaa tacatcaccc tgaccgtgcg gcggattgat gacggtgtcg
     3241 gctcccggtg gctgacgcgc gatgtaaaac gtggtgatta tctctggctt tcggacgcga
     3301 tgggggaatt tacctgcgac gataaagcag aagataaatt cctgttgctg gcggcaggct
     3361 gcggcgttac gccgattatg tcgatgcgtc gctggctggc gaagaaccgt ccacaggccg
     3421 atgtgcaggt gatctacaac gtgcgtacgc cgcaggatgt gattttcgcc gatgagtggc
     3481 gtaactatcc ggtaacgctg gtggcggaaa ataacgttac cgaaggcttt atcgctggtc
     3541 gtctcactcg cgaactgctg gcaggtgtcc ctgatttagc ctcacgtacc gtgatgacct
     3601 gtggccctgc tccatatatg gattgggtag agcaggaagt gaaaacgctc ggcgtgacgc
     3661 gtttctttaa agagaaattc ttcaccccag tagcggaagc ggcgaccagc ggtctgaaat
     3721 tcaccaaact gcaaccggca cgagaatttt acgctccggt tggcaccacg ctactggagg
     3781 cgctggaaag caataacgtt ccggttgtcg ccgcctgccg tgcgggtgtt tgcggctgct
     3841 gtaagacgaa agtgatttcc ggtgaatata cggtgagcag cacaatgacg ctgaccgacg
     3901 ccgaaatcgc tgaaggttac gtactggcct gctcctgcca tccgcagggg gatttggttc
     3961 tcgcataatc gcctgatgcc cgatgatatt cctttcatcg ggctatttaa ccgttagtgc
     4021 ctcctttttc tcccatccct tccccctccg tcagatgaac taaacttgtt accgttatca
     4081 cattcaggag atggagaacc atgaaacaaa cggttgcagc ttatatcgcc aaaacactcg
     4141 aatcggcagg cgtgaaacgc atctggggag tcacgggcga ctctctgaac ggtcttagtg
     4201 acagtcttaa tcgcatgggc accatcgagt ggatgtccac ccggcatgaa gaagtggcgg
     4261 cgtttgccgc tggcgctgaa gcacaactta gcggagaact ggcggtctgt gccggatcgt
     4321 gcggtcccgg caacctgcac ttaatcaacg gcctgttcga ttgccaccgc aatcacgttc
     4381 cggtactggc gattgccgct catattccct ccagcgaaat tggcagcggc tatttccagg
     4441 aaacccaccc acaagagcta ttccgcgaat gtagtcacta ttgcgagctg gtttccagcc
     4501 cggagcagat cccacaagtg ctggcaattg ctatgcgcaa agcagtgcta aaccgtggcg
     4561 tttccgttgt cgtgttaccg ggcgacgtgg cgttaaaacc tgcgccagaa ggggcaacta
     4621 cccactggta tcatgcgcca cagccggtag taacaccgga agaagaagag ttacgcaaac
     4681 tggcgcagct gctgcgttat tccagcaata tcgccctgat gtgtggcagc ggctgtgcgg
     4741 gggcgcataa agagttagtt gagtttgccg ggaaaattaa agcgcctata gtccatgccc
     4801 tgcgcggtaa agaacatgtc gaatacgata atccatatga tgttggaatg acgggattaa
     4861 tcggcttttc gtcaggtttc cataccatga tgaacgccga tacgttagtg ctgctcggta
     4921 ctcagtttcc ctaccgtgcg ttctacccga ccgatgccaa aattattcag attgatatca
     4981 acccagccag catcggcgcg catagcaagg tagatatggc gctggtcggc gatatcaaat
     5041 caaccctgcg cgcattgctg ccactggtgg aagaaaaaac cgaacgcaat tttctggata
     5101 aggcgctgga agattaccgc gacgcccgca aagggctgga cgatttagct aaaccgagcg
     5161 agaaagccat tcacccgcaa tatctggcgc agcaaattag tcattttgcc gccgatgacg
     5221 ctattttcac ctgtgacgtt ggtacgccaa cggtgtgggc ggcacgttat ctaaaaatga
     5281 acggcaagcg tcgcctgtta ggttcgttta accatggttc gatggctaac gccatgccgc
     5341 aggcgctggg cacgcaggca accgagccag aacgccaggt ggtcgccatg tgcggcgatg
     5401 gcggttttag catgttgatg ggcgatttcc tctcggtagt gcagatgaag ctgccagtga
     5461 aaattatcgt ctttaataac agcgtgctgg gctttgtggc gatggagatg aaagctggtg
     5521 gctatttgac tgacggcacc gaactatacg acaccaactt tgcccgaatt gccgaagcct
     5581 gcggcattac gggtattcgt gtagaaaaag cgtctgaaat cgatgaagct ctgcaacgcg
     5641 ccttctccat cgacggtccg gtgttggtgg atgtggttgt cgccaaagaa gaattagcca
     5701 ttccaccgca gatcaaactc gaacaggcca aaggtttcag cctgtatatg ctgcgcgcaa
     5761 tcatcagcgg gcgcggtgat gaagtgatcg aactggcgaa aacgaactgg ctaaggtaaa
     5821 aagggtggca tttcccgtca taataaggac atgccatgat tgatttacgc agtgataccg
     5881 ttacccgacc gagccgcgcc atgctcgaag cgatgatggc cgccccggtt ggggacgacg
     5941 tttacggaga cgaccctacc gttaatgctc tgcaggacta tgcagcagag ctttccggta
     6001 aagaagccgc catttttctg cctaccggca ctcaggccaa cctggtcgct ctgctcagtc
     6061 actgcgaacg cggcgaagag tatatcgtcg gtcaggccgc gcataactat ctgtttgaag
     6121 ccggtggcgc agcagtgctg ggcagtattc agccgcaacc catcgacgca gttgccgacg
     6181 gcacgctacc gctggataaa gtggcgatga aaatcaaacc tgacgatatc catttcgccc
     6241 gcaccaaatt actcagtctg gaaaacaccc acaacggcaa agtgctgccg cgtgaatacc
     6301 tgaaagaagc atgggaattt acccgcgagc gcaatctggc gctgcacgtt gacggcgcgc
     6361 gcatttttaa tgccgtggtg gcttacggct gcgaactgaa agagatcacg caatattgtg
     6421 actcgttcac catttgcctg tcgaaaggtc ttgggacgcc agtcggttca ttactcgtcg
     6481 gtaatcgtga ttacattaaa cgtgccattc gctggcggaa aatgacaggt ggcgggatgc
     6541 gccagtccgg cattctggct gccgccggga tgtatgcgct gaaaaataac gtcgcacgtt
     6601 tgcaggaaga ccacgataac gccgcctgga tggcggagca gttgcgtgaa gcaggcgcgg
     6661 atgtgatgcg tcaggacacc aatatgctgt ttgttcgcgt cggggaagaa aatgctgccg
     6721 cgttaggcga atacatgaaa gcgagaaacg tgctgattaa cgcctcgccg attgtccgcc
     6781 tggtgaccca tcttgacgtc tcgcgtgaac aactggcgga agttgccgcc cactggcatg
     6841 cgttcctggc gcgttaagga gagagacgtg ccgcaacgca ttttggttct cggtgccagt
     6901 ggctacattg gtcagcatct ggtgcgcacg ctcagccagc aagggcatca gatcctggcg
     6961 gcggcacgtc atgtcgacag aattgcaaaa ctgcaactgg ctaatgtcag ttgccataaa
     7021 gtcgatctca actggccgga taaccttccg gccctgttgc agaatattga tactgtctat
     7081 tttctggtgc acagcatggg cgaaggcggt gattttatcg ctcaggagcg ccaggtggct
     7141 ctcaacgtcc gcgatgcgct acgtgaagtc ccagttaagc aattaatctt tctcagttcg
     7201 ttgcaggccc cgccacatga gcagtcggac catctgcgcg cccgtcaggc tacggcggat
     7261 attcttcgtg aagcgggtgt cccggtgacc gaattgcgtg ccggaataat cgttggtgca
     7321 ggttcagcgg cgttcgaagt catgcgcgat atggtctaca acctgccagt gttaacgccg
     7381 ccacgctggg ttcgttcacg caccacgccc atcgcgctgg aaaacttgct gcactatctg
     7441 gtggcgctgt tagatcatcc ggccagcgaa catcgcatct tcgaagccgc cggaccagaa
     7501 gtgctcagtt atcagcaaca gtttgaacat tttatggcgg tgagcggtaa gcgccgctgg
     7561 ttgatcccca tccccctccc cacccgctgg atttcggtgt ggtttcttaa tgtgattact
     7621 tcagtaccgc ccaccaccgc cagggcgttg attcaggggc tgaaacatga tctgctggcg
     7681 gatgacaccg cgctacgtgc gctcatccca caacggctga tcgctttcga tgacgcggta
     7741 cgtagcacgt tgaaagagga agaaaaactg gtcaactcca gcgactgggg atatgacgcg
     7801 caggcttttg ctcgctggcg accagaatac ggttattttg ccaaacaggc ggggtttacc
     7861 gttaaaacgt ctgccagcct tcaggcgcta tggcgggtgg tcaatcaaat cggcggtaaa
     7921 gagcgttatt tctttggcaa tattttgtgg cagacacggg cgttgatgga ccttgcgatc
     7981 ggtcataagc tggcaaaagg ccgcccggag cgcgaatatt tacagaccgg cgatgcggtg
     8041 gatagctgga aagtcattgt cgttgaaccg gaaaaacaac ttacgttgtt atttggcatg
     8101 aaagcgccgg ggctgggacg actgtgtttt accctggaag ataaaggcga ctatcgtact
     8161 attgatgtcc gtgctttctg gcatccgcac ggtatgccgg ggctgtttta ctggttattg
     8221 atgatccccg cgcatctgtt tatttttcgc ggaatggcaa aacgaatcgc cagactggca
     8281 gaacaaagca cagattaatc gacaaaacct gcgttattct ttcatgtttc ccattgcatc
     8341 atggaggaaa tcacggaaga atgcgcacgt tattttctca acaacagtgg attgatatga
     8401 aggtactggt taccggcgcc accagcggct taggtcgaaa cgcggtagag tttttatgcc
     8461 agaaaggcat cagcgtgcga gcgaccggtc gcaacgaggc aatgggtaaa ttgctggaaa
     8521 aaatgggcgc agagtttgtt ccggcggatc tgaccgagct ggtctcatca caagctaaag
     8581 tgatgctcgc aggcattgat acgctgtggc actgctccag ctttacctcg ccctggggga
     8641 cacaacaggc tttcgatctg gctaacgttc gtgccactcg ccgcctgggt gaatgggctg
     8701 tcgcctgggg tgtgcgtaac tttattcata tctcttcccc ctccctgtac ttcgattatc
     8761 accaccatcg cgatattaaa gaagattttc gccctcaccg cttcgccaac gagtttgccc
     8821 gcagcaaagc agccagtgaa gaagtgatca atatgctttc gcaggcgaat ccacaaacgc
     8881 gctttactat tctgcgccca caaagtctgt tcggaccgca cgataaagtc tttatccctc
     8941 gtctggcgca tatgatgcat cactacggca gtattctgtt accgcatggc ggcagtgcgc
     9001 tggtcgatat gacctactat gaaaatgccg tgcacgccat gtggctggca agccaggaag
     9061 cctgcgataa gctaccttcc gggcgtgtgt acaacatcac caacggcgag catcgcacac
     9121 tgcgcagcat cgtgcagaag ctgatagacg aattgaatat tgactgtcgt attcgttccg
     9181 tcccttaccc gatgctggat atgatcgccc gcagcatgga gcgtttaggc cgcaagtcag
     9241 caaaagagcc gccgctgacc cactacggcg tctccaagtt gaattttgac tttacgctgg
     9301 atattacgcg tgcgcaggaa gagttaggtt atcaaccagt cctcaccctg gatgaaggta
     9361 tcgagaaaac tgccgcctgg ctacgcgacc acggaaaact gccgcgctaa tcctgcccgt
     9421 atttctccag caacgcttcg gcaatcgcct gagtttctgc atccgcttcg ccgttatata
     9481 acgtcgggcg gaaatgcatc tggaacgcca taatcacgcg ccgctgctcg cgcggagtca
     9541 tatcaggctt tacgtcataa ccataacgcg ccaaaagctc cagcaatgac gcggtctcta
     9601 ccggtgtgtg cggcgcgcgc ccggcaaggt aaaagttcac ccgctgcgca tccggccatg
     9661 caccaatccc ctgctgtgcc agttgctgcc aggggaataa tggccccgga tcgtctttgc
     9721 gctgcggcgc aatatccgca tgtgctacta cgttttccgg cttgatgtga taacgggcaa
     9781 taatatcttt cgccagcgga ataagcgcct gaatctgtgc cggttcaaac ggggcaaaat
     9841 atttcactcc cgccgatttt tgccagccac ggttttccag ctcaatgcca atagaggtgt
     9901 cgttaaggcg cgttgccccg cgccaggcgc taatccccgc atgccaggcc agttcttgtt
     9961 ccggcaccag ttgccagatg cgcggtttac cgttgtatcg cggtggcacc gcagggacca
    10021 gataatgcga gctgacctgc ttatcggtca gtgtcgccag cgagctatca aaatcatctg
    10081 cggtgtagtg gatcaccagc actttaatgc gcggatacgc cgcctgcgcc tggcgtcggg
    10141 tatcaagctg atatccctct ttctcgacaa tgcctttttc gcctgtgcac cctgccagta
    10201 acagagcggc agcgaccagc cagaagactc ttctcatcga cgcgttttca ccgccgtacc
    10261 gctgacgcta accatcagca tactgccgtt ttgcccgacc gtttcgtagt cgatatcaat
    10321 accgacgacg gcatcagccc ccagcgctcg cgcctgggag ccgagctcct caaaggcaat
    10381 ctcccgcgct ttacgcagtt ctttttcgta ggcaccggag cgtccgccaa cgatatcgcg
    10441 gataccggca aagaaatcac ggaaaatatt cgcgcctaaa atcgcttcgc cagtcaccac
    10501 accgcaatat tcaacgatgg tctggccttc cagagttggg gttgtagaaa attgcatgat
    10561 cgatctcctt gtttgctaag ccttcgatct tcaaagcatt atcggactga tacgctatta
    10621 ttgaaaggat atcattatta tcgtagaacg ctttcaaggt gttcgcttat aaggaaatca
    10681 tcatgcgcta tagcaaattg accatgctta tcccttgcgc actgctactc agtgcctgta
    10741 ccaccgtcac gccagcttat aaagataacg gcccccgcac cggttcctgt gtccaggggg
    10801 ggcctgatag cgtggctcaa cagttctatg actaccgcat tcaacatcgc ggcaatgaca
    10861 ttaccgctct gcgcccctat ttgagtgaca aactggcaac cctacttagc gatgccagcc
    10921 gcgataatag tcatcgtgaa ttgttaagca gcgatccgtt ctccagtcga accaccttgc
    10981 cagacagcgc ccacgttgcc agtgcatcta ctatccctaa tcgtgatgcc cgtaacattc
    11041 cattgcgcgt ggatctcaag cagggtgacc agggctggca ggatgaagtg ttaatgattc
    11101 aggaaggcca gtgttgggtc atcgacgatg tgcgctactt aggcggtagc gttcatgcca
    11161 cggcgggtac gctccgtcag tccatcgaga atcgctaacg tcttgatcaa ccaataaacc
    11221 ccgtaaattg tccggcattt cgtgaggaat gccgacattt atgctcgccg accaccgccc
    11281 ccgttatttt gtgctatgtt tattgaataa tgcgctttgc ttttaacttt taaagcagaa
    11341 atattgcata attattctgt caaaggtact atcttcgacc tcaatagcta tcagactgcc
    11401 agtatacgag tgtcaatgag tattcaatta aacggcatta attgcttcta cggcgcgcat
    11461 caggcgctgt tcgatatcac gctggattgc ccacagggcg aaacgctggt gttacttggc
    11521 cccagcggcg cgggtaaaag ctcgctgctg cgtgtactca atctgcttga gatgccgcgc
    11581 tccggtacgc tcaacattgc aggcaaccat ttcgatttca ccaaaacccc ctctgacaaa
    11641 gcgattcgcg atttgcgtcg taacgttggc atggtgtttc agcaatacaa cctgtggccg
    11701 catctgaccg tgcagcaaaa cctgattgaa gcgccctgtc gtgtactggg gttgagtaaa
    11761 gatcaggcgc tggcccgtgc agaaaaactg ctggaacgtc tgcgtctcaa accttatagc
    11821 gatcgttacc cgctgcatct ttctggaggt cagcagcaac gtgttgctat tgcccgtgcg
    11881 ttgatgatgg aaccgcaggt actgctgttc gatgaaccga ccgccgcact ggaccccgaa
    11941 attacggcac aaatcgtcag catcattcgt gagctggcag aaacgaatat tacccaggtg
    12001 atcgtcaccc acgaagttga agtggcgcgt aaaaccgcca gccgagtggt gtatatggaa
    12061 aacggtcata tcgtagaaca aggcgacgcg agctgcttta ccgagccgca aaccgaagca
    12121 tttaaaaact atctctctca ctaagattcg ggataacaac aatgaaaaaa gttctgattg
    12181 ccgcgttaat tgcaggtttt agtctttccg ccacagcagc cgaaaccatt cgttttgcca
    12241 ccgaagcctc ctatcctccg tttgaatcga ttgatgcaaa caaccagatc gttggttttg
    12301 atgtcgacct ggcgcaggca ttgtgtaaag agattgatgc aacctgcact ttctctaacc
    12361 aggcgtttga cagcctgatc ccaagcctga aattccgtcg catagaagca gtgatggcgg
    12421 gcatggatat cactccggag cgtgaaaagc aggtgctgtt taccaccccg tactatgaca
    12481 actctgccct gtttgtgggt cagcaaggca aatacaccag tgttgatcag ctgaaaggca
    12541 aaaaagtcgg cgttcagaac gggacgacac accagaagtt cattatggat aagcatccgg
    12601 aaatcactac cgtgccatat gacagttacc agaacgcaaa actggatctg caaaacggtc
    12661 gtatcgacag cgtatttggt gacaccgcag tggtaaccga atggctgaaa gataacccga
    12721 aactggcggc agtgggcgat aaagtgaccg ataaagatta cttcggtact ggcctcggca
    12781 tcgcagtacg ccagggcaac actgaactgc agcagaaact caacactgcg ctggaaaaag
    12841 tgaagaaaga tggcacttac gaaaccatct acaacaaatg gttccagaag taattcctga
    12901 tgaatgaatt ttttccttta gcaagcgccg ccgggatgac cgtcggcctt gccgtttgtg
    12961 cattgattgt cgggctggcg ctggcgatgt tctttgcggt atgggagtct gcgaaatggc
    13021 gtcctgtcgc gtgggcaggt tcagcgctgg taaccattct gcgtggcctg ccagaaattc
    13081 tggtggtgct gtttatctat tttggctcct cgcagctgct gctgacgctt tcggatggct
    13141 tcactatcaa tcttgggttc gtgcagatcc cggtgcagat ggacattgag aacttcgacg
    13201 ttagcccgtt cctttgtggt gtcatagcac tgtcactgct gtatgccgcc tatgcctcgc
    13261 aaacgctgcg tggtgcatta aaagcggtgc cggtggggca gtgggaatcc ggtcaggcgc
    13321 tggggctgtc gaaatcggct atctttttcc gtctggtgat gccgcagatg tggcgtcatg
    13381 cgctgccggg tctcggtaac cagtggctgg tgctgctgaa agataccgcg ctggtcagtt
    13441 tgattagtgt gaatgattta atgctacaaa ccaaaagcat cgctacccgt acccaggaac
    13501 cgtttacctg gtacattgtg gcggcggcga tttacctggt gatcaccctg ctcagtcagt
    13561 acattctcaa acgcattgac ctgcgcgcga cacgttttga gcggaggccc agctaatgtt
    13621 tgagtattta cccgaactga tgaaagggtt gcacaccagc ctgacgctaa ccgttgcctc
    13681 gctgattgtg gcactgattc tggcattgat ttttaccatc atcctgacgc tgaaaacgcc
    13741 ggtgctggtg tggctggtgc ggggctatat cacgctgttt accggtacgc cgctgctggt
    13801 gcagatcttc ctgatttatt acggaccggg ccagttccca acgttgcagg agtatccggc
    13861 actgtggcat ttgttgtcag aaccgtggtt atgtgcgctg attgcgttgt cgctgaacag
    13921 tgcggcgtac accacgcagc tgttttacgg tgcgattcgc gcgatcccgg aaggtcaatg
    13981 gcagtcctgt agcgccctgg gaatgagcaa aaaagatacg ctggcgatcc tgctgccgta
    14041 cgcgtttaaa cgctcgctct cttcttattc caacgaagtg gtgctggtgt tcaaaagtac
    14101 ctctctggct tacaccatta cgctcatgga agtgatggga tacagccagt tgttgtacgg
    14161 acgcacctac gatgtaatgg tatttggcgc ggcggggatt atttacctgg tcgtcaacgg
    14221 cctgctgacg ctgatgatgc gtctgatcga gcgtaaagca ctggcattcg aacggcggaa
    14281 ttaatgtagc tgcatgctga tgccggatgc ggcgtggacg ccttatccgg cctacatagt
    14341 gcataaattc ttattatgaa gacggacaac ccactaagtt gtccgtcttt ttttatttca
    14401 tacaaattat ttactcgcat ttattgcata taaattcact tgatggcatt gttatcccat
    14461 gccgcagaca cggccaaaaa tcataagatt gacagacggg agttccatca tgaaaaagtt
    14521 agttcttgcc gcattactta cttcctttac tttcggtgct tctgccgcag agaaaatcaa
    14581 ttttggcgtt tcagccacct atccaccctt tgaatctata ggtgctaata atgagattgt
    14641 cggctttgat atcgacctgg caaaagcctt gtgcaaacaa atgcaggcag aatgtacttt
    14701 tactaatcac gcgttcgaca gcctgatccc gtcactgaaa ttcagaaaat atgacgccgt
    14761 aatctccgga atggatatca cgccggagcg tagcaaacag gtatcgttta ccacgcccta
    14821 ctacgaaaac tcagccgtcg tgattgccaa aaaagatacc tacaaaacgt ttgccgatct
    14881 gaaaggcaaa cgtattggga tggaaaacgg caccacgcac cagaaatata ttcaggatca
    14941 gcacccggaa gtaaaaactg tctcctatga cagttatcag aatgccttta tcgatctgaa
    15001 aaatggccgt attgatggcg tatttggtga caccgcagtg gtaaacgaat ggctgaaaac
    15061 caatccgcaa ctgggcgttg ctactgaaaa agtgaccgat ccgcaatact tcggcaccgg
    15121 cctgggtatc gctgtacgtc cggataacac agctctgctg gaaaaactga ataacgcgct
    15181 ggcagcaatt aaagctgacg gtacatatca gaaaatcaat gaccagtggt tcccacagta
    15241 agattcagaa agccgcacct gcggcttttt tattgcttca ccagtagcgt cagcacttca
    15301 tagtgcgcgg tgtgcgggaa catatcaaaa agctgtaccc gttcgatgcg ataaccaggc
    15361 agttcgcgga tatctttcgc catagtttgg gcgttacagc tggagtagat aataaaacgc
    15421 ggtgccatcg ttgagagata atcacacagc ggtttaccga tgccgcggcg cggcgggtta
    15481 accagcacca gctccggcac ttccccctga gcggtggcaa actgcgtaga gtccagcgcc
    15541 tgaaattgca aacgcgttaa gcccagttca gcggctgact gcttcgcaca ggcaatggcc
    15601 tctggtgcaa tttcgatccc ggttaactgc atgtcaggcg tcgcgcagtg taaaccaaag
    15661 ccacccacac cacagaacag atcccacata tgtttaaccg gcagctgtcg tacccagtcg
    15721 cgcgcggtgg cgtacagctg gctggcgacc gccggattag tctggaagaa actttgcgga
    15781 cggatccaca gcggtacgtc attaaaacgc tctgccagtg cctgttgttc ggtcaggtag
    15841 atctccgtct ccccttccat aatcgccata tgtaccggct gaatattgac ggtaataact
    15901 ttcagctgcg gtaattgttc ctgtaaccac ggcagcgcct tacgcagttg cgccagtttg
    15961 gtatcagaac gcagtacaaa acgcagcatc atgccgccat cgctctggct ttcagtcagc
    16021 agaatgtatt tcagttcacc acgtttacgc gccacgttgt aaggcgttaa acccgcacgg
    16081 gcgataaacg gttttagcgc cgcaaaaacg ggcgcaaatg aggctggata aagcgggcag
    16141 tcacaaaggt cttccggtgt accatcccga tgcagcatac cgagcagtgg tttttcaacg
    16201 ctaccactca ccaccatttt ggctttatta cgaaaccctt gttccgggcc tgacaccggc
    16261 gcacaccatt cctcaaccgg aaaatcggcg agcagatttt taagatcgac ggttttagcg
    16321 gagagttgct ctggaatcgg ctgcgttatc cactgacagg aacgacagcg acccgcgtcg
    16381 taaagtgcgc actgcataca ttgaccttca catcatcagg ggcgacgatt atacacatta
    16441 ttgcaactgg aagaaccgcc gactggtcga gggaacgaac agcagcatca ggatgagcat
    16501 atccggcagc ttctgcagca tcaggctatg gaagatttca cgttttgatt caccgggaat
    16561 gctgaacagc tccggataac cataccccag cgaggccgcc cacaagtaac ttgcggctgt
    16621 gatttgtgtc aacagataca gccagcgcgc ccagcgacgc cctttcacca gtgaaaacgc
    16681 acaccagatc tcaatgaaaa ccagcaccag actgctcaaa aagaccagcg ttaaactcca
    16741 ggtttgtacg ctgcgatgaa tgaactcgcc aataccgtgc acgcccagcg tattgaaaat
    16801 catcagcacg tcgaggccac ggatcataat aatggcgagc gccgccacct gcaccagcgc
    16861 aggcacattc aggcgagcat gagatgaaga tgttttctta aaaaatccca acgtttcgtc
    16921 ttccatgaaa acaatgccgc gacatgcgcg gcattatgta gccaggttgg caaattttag
    16981 tgtcttcagc cacgtcttgc acgctggatg tcgcgtatcc gctgtttttc tgcgcgcgcc
    17041 atcagatacc aggcgataaa tccgacaatt ccgaccgcgc cgaggatcaa tgttgccagg
    17101 gcgttgattt ccggattcac ccccatccgc acgctggaaa agaccagcat cggtaacgtg
    17161 gtggctcccg gcccggaaac aaagctggcg ataaccagat catcaagcga caaagtaaaa
    17221 gccagtaacc agccagaaat gatcgcgggc atgatcatcg gtagcgtaat gacgaaaaac
    17281 actttcagcg gcgtcgcacc aagatccatc gctgcttctt ctatcgagct atccagttcc
    17341 cgcagacgcg acgaaataac gactgccaca taagccgtac agaaagtgac atgcgccagc
    17401 cagatggtga gcataccgcg gtccgcaggc cagccaatgg catgagcaag cgcgacgaat
    17461 aacaacaata gcgacaagcc cgtgatgaca tctggcatca ccagcggcgc ggtgatcatg
    17521 aaggcaaaac catttgatcc gcgaaacctg ccaaagcgca ccagcacgac cgccgcaata
    17581 gtcccgagga tcgccgccgc cgtcgccgca caggccgcaa ttgtcagact taaagcaacc
    17641 gcactcatca tcgcgtcatc gcgcaataat tcgccatacc agcgcgttga ccagccggcc
    17701 cacaccgtca ctagcttcga gctgttaaac gaatagataa caagcatcag cattggcgcg
    17761 tagagaaagg tgaagcccaa cagcaaaatc acaatccgcc agggcgaacg aaccaccggt
    17821 aaattattca tccgtgttct cccacgcttt tttgctgatg tttgtggaac cacattatcg
    17881 gcataattag cagcagcaac atgatgatcg ctaccgctga ggccaccggc cagtcgcggt
    17941 tgttaaagaa ctcctgccac agcacgcgcc cgatcatgat gctgtccggg ccaccgagca
    18001 gttccgggat cacaaactcg cccacagccg ggataaacac cagcatcgat ccggcaataa
    18061 tcccaccttt agtcagcggc acgatcacag taaagaacgt tttcagcggt cgtgcaccga
    18121 gatccagtgc cgcttctacc agcgaataat caatacgaat caacgcggtg taaatcggca
    18181 gcaccataaa cggcacgtag gcgtaaacaa tgccaatata aaccgcaaag ttggtatgca
    18241 gaatggtcag cggttgatcg ataatcccca gccacagcag aaaattattc agcacaccgt
    18301 tgttttttaa tattcccatc caggcgtaga cacgaatcag aaacgaggtc cacgacggca
    18361 ggatcaccag tagtaataag atattacggg tcgaaggctt actgtgcgcc accgcccacg
    18421 ccagcggata gccgatcagt aaacagcaaa tagtcgaaat cgccgccacc tgtaacgact
    18481 ggagataagc atcaaaatag agcggatcgt cggtcagttg cagaaaatta ccgagattaa
    18541 gagtgataga aagttgcccg tcagcccact ccatcaattc ggtataaggt ggaatagcgc
    18601 gcgccatctc cgcaaggctt attttaaaga cgatcagaaa tggcagcaga aacagcaaga
    18661 tcaaccagat atatggcaac gcaatgacca gtttgcgccc atgcttcatt tgcagctgcg
    18721 acaaccacag cttaaagggt tttggctggg cagcaggttc aagtgtactc atctttgctc
    18781 cttaaaccgt cagcaccaca cagctgtcca cttcccagca caaacgcact tcgtcgcccc
    18841 aggtcggtaa ccctttacga tggcgatggg cgttttgtag ctgggcgcta atcatctgcc
    18901 cacttttcag gcgaacgtga tacaccgaaa gatcgccgag ataggcaatg tgtatcacct
    18961 cccccaccgc gaagttacaa ccattggcgg gcggttcctc gcaaagcatg attttttccg
    19021 ggcgaagcgc cacatgcacc ggcacgttat cgacaaccga agcatccaca tcgactttca
    19081 gtggatgcac cagccccggc gaataaagca ccagaccatc ttcctgacgc tctttgagta
    19141 caccttcaaa gacattaacc gaaccgataa attcagcgct gtagcgagtg gtcggatgct
    19201 cgtagatctc ttccggctcg ccaatctgga caaatttccc acgattcata atggcgatgc
    19261 gccccgccat ggtcatcgcc tcttcctgat cgtgagtaac cattacgcag gtcacaccga
    19321 cgcgctccag aatatccacc acttcaagct gcatccggtc acgcagcttt ttatccagcg
    19381 cgcccatcgg ctcatcgagc agtaatagtt tcgggcgctt ggcaaggctt ctggccaggg
    19441 ccacacgctg acgctgacca ccggaaagct gatgcggttt gcgtttggcg aactcctgca
    19501 tatgcaccag cccaagcatc tcattgaccc ggctggcaat ttccgctttc ggtagtttgt
    19561 cctgtttcag gccaaaagcg atgttctgtt ccacggtcat atgcggaaac agcgcgtaag
    19621 actgaaacat catattgatg gggcgcaggt aaggcggcac ctgtgacaaa tcgacgccat
    19681 cgagcataat ctgcccggcc gaaggttgtt caaaacctgc cagcatacgc agcagcgtgg
    19741 acttgccaca gccggatgcg cccagcagcg cgaagatttc acctttgtag atggtcaggc
    19801 tgacatcatc caccgcatgt tgaccatcgt aggatttggt caggttacgg atttctaata
    19861 gcggcgtcag cgccttacgg gttttcgcct gcgggcgagg gatagcgtca ttcacggctg
    19921 ctctccggca ttgaatgtgt gcaagcgcac catgatggtg cgaattgccg gcgggtgtgg
    19981 tgcgcccgtc cggcatctac ggctgcggat tattttccgc tcttcacttt ggtccacgcg
    20041 cgggtgcgca cacggtcgat tttcgggtcc tgcactttca gagtgaacag cttcgcacga
    20101 acatccgcag gcggataaat acccgggtta tcacggactt ccgcactcac cagcggcgtg
    20161 gctgctttat tggcgttagc atagaacaca tggtcagaga tatgtgccac cacatcagga
    20221 cgcagcaggt aattcaggaa ctgataggct tcgtctttgt ttttggcatc cgcaggcatg
    20281 gcgaatacat caaagaacgc catcgcccct tcttttggaa tcgagaacga gacattcacg
    20341 ccattcttcg cttccttcgc gcggtttgac gcctgccaga catcgcccgc ccagccaata
    20401 gcgacacaga tgtcaccatt cgccagatca ttgatgtatt gggatgaatg gaaataacga
    20461 atgttcgggc gcagctttaa cagcagatcg gtagccggtc cggtgtaatc atccgcttta
    20521 gtgctgttag gatctttgcc gagataattc agcacggtag caaacacttc ttctggcgca
    20581 tccaggaaag agacaccgca gcttttcagt ttttccagat tttcaggttt gaggatcaag
    20641 tcccagctat cgacgggcgc gttttcgccc aacaccgctt taactttatc gacgttatag
    20701 ccaatcccgg tcgtcgccca catatagggc atagcaaatt tattgtcggg atcgtgtttg
    20761 gcgaccaact tcagcagctc cggatcgaga ttcttccact ccggcaattt gcttttatcc
    20821 agcggctgga aaactcccgc tgtcagctgg cgctcaagaa agcttgctga cggcaccacc
    20881 agatcaaagc cagtgctccc cgccattaat ttgccttcca gtacttcgtt agaatcgaaa
    20941 acatcgtaga caactttaat accggtttct ttttcaaagt tggccaccgt gtccggggcg
    21001 atataatcag accagttata aatgtggagt gttttttgtt cagccgcgag cgtgctgaca
    21061 gagacggcca tcagagcccc cgcaaccaga cccgatagcc attttttatt taaggcggtc
    21121 atatctcttt ccttctgaaa attcgttaac aaacgaacaa aagttgtcac actgaaagat
    21181 atgcaataaa cgtgcatatt tttatcggcg cggtgtaaga aaagagaact ctctcccggc
    21241 ctgcactgct cactatataa aagcatcgca agaataactt tagccagggt ttgagactat
    21301 agctcggatt aaaaaacggt atttaacaaa tttttatgcg tatttgctct atgtgataaa
    21361 gccaaatcgg cacaagagaa gtgaattaat ctttaataaa aggttaaaaa cagcggataa
    21421 ttttgcggca cgcagtcact gcggcagcga ctgcgtgctc gaaaggagtt atcagtggag
    21481 gaaatagttt tcggtaacgt tattttgccc ttcatcgtca gtcggtaaca gcagttgatg
    21541 ggcattagct tcaagaatga ccatcgaaat ctgctcttcg ctctgacgca cgaaccatgc
    21601 aaactgctca taggttacgc cctgcataac ggataaagac tggcaaacca ccagctttgg
    21661 cagattatca tcctgcatat caagaaatgc tttcacggtc agcgaactgg cattgatggc
    21721 tgataaatcc gccgccagcg gcaatacggc tgaaggtcgg acttccgcca tggctgaaaa
    21781 caggatcgtg ttatcgatca gatcgatttt agcatcaaag acaccgtcga aattctgcat
    21841 atggggcaga tgcagagcct gacagttatc acattcaaaa aaactcatcc ccaggtcatc
    21901 gagccattga cgcaacgtat ccagaccagg aacgaccagc gatgtcataa ttttgtacaa
    21961 cctcttccga taaaaagacc ggcacagctt acgcaaaaag cgcaggcaaa accatgatca
    22021 gtaatgtgat tgcgactaac cacccgtttt caggcaatat tctgtcgtag cgtggcgttc
    22081 gatccagcgg atcattttac ccgcgatgtc gataccggtg gttttttcta ttccttccag
    22141 ccctggcgac gcattcactt ccatcaccaa cggcccgcga ttagcacgca gaatatccac
    22201 accagcaacg tccagcgcca tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg
    22261 tgtgatactg gcgacgcttg ccgcgccgcc gcgatgcaaa ttggaacgaa aatcgccctc
    22321 tttcgcccgc cgttcaatcg cagcgactac ttcatcgcca acaaccagac agcggatatc
    22381 gcacccttgc gcctctttga tatattcctg caccagaata tgcgcgttca gaccgcggaa
    22441 agcgtcaatc acgctttccg ccgcctgacg cgtctccgcc agcacgacgc caattccctg
    22501 cgtgccttca accagcttga ccaccagcgg cgcaccaccg accatgtcga ttaaatcgct
    22561 ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg tcgatgccct gacgcgccag
    22621 cagttgcatg gaacgcaatt tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg
    22681 atagctcccc agcatctcga actggcgcag tgccgccgtc ccataaaagg taatggcggt
    22741 gccaatacgc gggatcactg cgtcaaaatg gggtaacttg cggcctttgt agtgaataga
    22801 agacgccgca ggatttatgt tcatgtagca agaaagcgga tcaagaattt caaccaggtg
    22861 accgcgctgt atagcggctt cacgcagccg cttacacgaa tagagcgttc catcccggga
    22921 caatatggca attttcaccc tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca
    22981 tacatcgacg tggcagtttt agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat
    23041 aaatgggcgg ctttctttga tgattgttcg gcggatatga tcgctccagg tatcccggcg
    23101 attattgctg ccacgggtga ggtaatattg cgccagttgc tcgtcatact gcgccagtgc
    23161 gtctttatcc agcggttgat agctgttttc atgcaccaaa atggaggccg gtaaacgcgg
    23221 cttaagatcc ggattatccg caggccagcc aaggcacagc ccaaacagcg gcagaacatg
    23281 ctgcggtaat ttcagcagtt ccgtcaccgc ttcaatatta ttgcgcaggc cgccgatata
    23341 taccccgccc aatcccagcg attccgctgc ggttaatgca ttctgcgcca tcattgccgt
    23401 atcaacgaca ccgagcaaca gttgttccgc caggccgagc tgagcatccg gacagatctg
    23461 taaatggcgg ttaaagtcgg cacagaacac ccaaaactcc gccgcttgtg ctacgtgttt
    23521 ttgcccgccg gtcagcgtca ccagttcttc acgtaacgct ttgtcggtaa tgcgaataat
    23581 gctactgcac tgcaaaaaac tggaactgga cgtcgcacgg gcgctgttaa taatcgcctc
    23641 acgctgcgct tcggaaatgg gttcatcagt gaaatggcga atggagcgat ggccacaaat
    23701 aagttcaatg gttggcgtca ttatcttttt ctctttctga acgtgaatat tgcggtggat
    23761 ggttcatcag ctgtggggca agacgttttg ccacctgaag aataaccacc accgcagcgg
    23821 gaagcatgag caaaacaccg agaaaaatca tcagaatctg cacttctggc cgagaaaatg
    23881 gctcaggtag cgacagggag tcgcttaccg acagcagcgc caccgccagc aacatcattc
    23941 cgataaattc cagtatcaac acgcttttag gcaatttacc gatcgcgcgc atacgcttcc
    24001 ctctgcaaag tgagccttca gtctaaaact tttcactgta ttgtgtttaa cagttatagc
    24061 ttttagcaat taatgcaaca ggttaaacct actttcagcg aatacatttt agcgtgatca
    24121 ttacaggcat aaatctatga ggagagaaat aatgcaaacc gttatttttg gtcgtccggg
    24181 ttgcccttac tgtgtgcgtg caaaagatct ggctgagaaa ttgagcaatg aacgcgatga
    24241 tttccagtat cagtatgtag atattcgtgc ggaagggatc actaaagaag atctacaaca
    24301 aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt gtcgatcagc aacatatcgg
    24361 cggctatacc gattttgctg catgggcgaa agagaatctg gacgcctgat cgtctgacaa
    24421 gccctcgcgt tgagggcttt attgattttt tctgtgctgt ggtttaaaca aactactgat
    24481 aaataagaaa cacaatgccc ccagcgcaca ccagaacacc gcacttaata accatgccag
    24541 ctcttgccag aatgagcgcg tcggtgaaaa aaacagccgc ataatgagca tcgaacaggg
    24601 tgccgccagc attgcgccaa acagaggttt caggacttct ctacgatgtg aaaagaagct
    24661 ggcaactgca ccaggaagaa tgaaaaatag caaaccgatt tcaggatgcc cggcagcccg
    24721 aaaagcgcct ttcatgtgcg tcgccagaaa aagacacacc acaatgaaga ggacaaaaca
    24781 gcagattgcc cccgcccaac gttgtttatg tttcactcgt tcctcctgac actgcgtcta
    24841 tcgaacacat ttttcgccag tgtggcgttc agtaagataa agccgcttcg cattccatgc
    24901 taatataggc caacgcaatt catatagccg ttgataccta atgtgattac actagtaaaa
    24961 tatattgtta ctttactatc gtttaggtgc gctgaatgaa tctgcgccct gaattctggt
    25021 aaaaaacatt atcgtaaatt accatttctt tcaacagctt actagtaaac aagaagttag
    25081 cctccgtgaa tataaacgtc gccgaattgt taaatgggaa ttacattctg ttattatttg
    25141 tggtcctcgc gcttgggcta tgtctcggga aattacgact tggttcgatc caactgggta
    25201 attccattgg cgttttagtc gtatcgctgt tattaggcca acaacatttc agcattaaca
    25261 ccgacgcgct taatcttggc tttatgctgt ttattttctg cgtcggggtc gaagccggac
    25321 cgaacttttt ttccattttt tttcgcgatg ggaaaaatta cctaatgtta gcactggtga
    25381 tggttggcag tgcgctggtg atcgccttag ggttaggtaa gctgtttggc tgggatattg
    25441 gcctgacggc cggtatgtta gctggctcta tgacgtcgac accggttctg gtcggtgctg
    25501 gcgatacact gcgtcattcc ggcatggaaa gcaggcaact ctcactggca ctggataatc
    25561 tgagcctcgg gtatgcctta acctatttaa tcggtctggt gagtttgatt gttggtgcgc
    25621 gttacttgcc gaaattgcag catcaggact tacagaccag cgcccagcaa atcgcccgcg
    25681 aacgtggcct ggacactgat gccaaccgta aggtttattt accggtgatc cgcgcctacc
    25741 gcgtcggccc ggagctggtg gcctggaccg acggcaaaaa tctgcgtgaa ctgggtattt
    25801 atcgacaaac tggctgctac attgaacgta ttcgacgtaa cgggattctg gcaaatccag
    25861 acggtgatgc tgtgctacaa atgggcgatg aaatagcgtt ggtaggctat cccgacgccc
    25921 acgcacgact cgatcccagc ttccgtaacg gcaaagaagt tttcgatcgt gaccttctcg
    25981 acatgcgtat cgtcactgaa gaagtggtcg ttaaaaacca taacgctgtt ggcaaacgcc
    26041 tggcacaact gaagttgacc gatcacggtt gcttccttaa ccgcgtcatt cgtagccaga
    26101 ttgagatgcc gatagatgac aacgtcgtgc ttaacaaagg tgacgtttta caagtcagcg
    26161 gcgatgcccg tcgcgtaaaa accatcgccg atcgcatcgg ctttatctcg attcacagcc
    26221 aggtcactga cttgctggca ttctgcgcct tctttgttat tgggctgatg atcgggatga
    26281 tcaccttcca gttcagcaca ttcagtttcg gcatggggaa cgctgccggg ttgttattcg
    26341 ccggaattat gctgggcttt atgcgcgcta accacccgac cttcggttac attccgcagg
    26401 gtgcattaag catggtgaaa gagttcggct taatggtgtt tatggcaggc gttggtctga
    26461 gcgccggtag cggtattaat aacggcctgg gcgcgattgg cggtcagatg ttgattgccg
    26521 gattgattgt cagtcttgtg ccagtggtta tctgtttctt gttcggtgct tatgtattgc
    26581 gaatgaaccg cgcactgttg ttcggcgcaa tgatgggcgc acgcacctgc gcgccggcaa
    26641 tggagatcat cagtgataca gctcgcagta acatcccggc gctgggctat gcgggcacct
    26701 atgcaatcgc caacgtcctg ctgacgctgg cagggacaat catcgtcatg gtatggccag
    26761 gattaggata aaactgaagt tgccctgaaa atgaaatttt tttgcacaac cgcagaactt
    26821 ttccgcaggg catcagtctt aattagtgcc actgcttttc tttgatgtcc ccattttgtg
    26881 gagcccatca accccgccat ttcggttcaa ggttgatggg ttttttgttg cctgaaattt
    26941 atctactacc cagcaatcct ctcaaccatc ctcaaaatct cctcgcgtga tagcggctta
    27001 cggtcggtga caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt
    27061 ccgggttcaa accattgttc gagcgtttgt tgactgcgct gcatccagtt ttgcattacc
    27121 gtttttaaga gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta
    27181 tccggcgttg caacctgtga gctgtagatc atatcggtga tagcctggca tgcgcctgga
    27241 gcatccctaa catcgctaaa aaatgcctga tattgccggg acatgatctc agtaaaacgg
    27301 ctgaacgcct ccagcaacaa ctcatcaatg cctgaaaaat agtaggtcat cgatcccaac
    27361 ggcaccccgg caagggtagc aattttgcgg tgtgtaacag catgtattcc gtaaagtttc
    27421 accgcctcca gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc
    27481 atgtcatttc ctctctctgc aattgtgtac aaatgtacac agtcttgcta atgttgtctt
    27541 ctctcttctt attttgacgt ggtctatgac cgtaaattct tcacgtaatg cattgaaacg
    27601 ccgaacctgg gcgctgttta tgttcttctt tttgccgggc ctgttaatgg cgtcctgggc
    27661 aacccgtacg cctgctatcc gcgatattct ttctgtttcg atcgctgaaa tgggcggagt
    27721 cctctttggt ctgtcgatcg gttcaatgag cggtattctc tgctcggcat ggttagtgaa
    27781 acgctttggc acgcgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat
    27841 gatattaagt ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt
    27901 ctttggggca agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ctgccgttga
    27961 gcgagaaatg aataagacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct
    28021 ggcaggcgct ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat
    28081 tttattggcg gcgctggtag gcatcgcgcc tatttatatc gccattcagg caatccctga
    28141 cggtacgggc aaaaatgctg ccgatggcac ccagcatggt gaaaaaggcg taccttttta
    28201 tcgcgatatc cagttgctgc tgattggtgt tgtggtgctg gcgatggcct ttgccgaagg
    28261 ttctgccaac gactggttac ccttattaat ggttgatggt cacggtttta gccctacttc
    28321 cggctcgctg atttatgccg gttttaccct ggggatgacc gttggacgct ttaccggcgg
    28381 ttggttcatt gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc
    28441 gttgggtatt gggctgatta tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt
    28501 actttgggga ctgggtgcct cgttgggctt cccgctgact atttctgccg ccagcgatac
    28561 cggccccgat gcaccaaccc gcgtcagcgt ggtagctacg accggttatc tggctttcct
    28621 cgtcgggccg ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct
    28681 ggttgtactg gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga
    28741 taccaaaacg cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac
    28801 atggatggta ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag
    28861 tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat
    28921 cagttgatct ctttcttccc tgaaatcgct aatgaaatag cctttgtggc tgaaaacggc
    28981 ggctgggtag tgagcgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt
    29041 gctactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa
    29101 aatagcgcct ataccctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
    29161 tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatctt ctttaaattt
    29221 ggccttaatc tctctgacga actgatccca caagtgcaaa aagcattaca tgaggccatc
    29281 ggcgatatca tggtaccggt ccataccggc aacggcagca tcgatctgat tatccccggt
    29341 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
    29401 gtggtggtct ttggcgatgg tggtaacgat attgagatgc tgcgtcaggc aggctttagt
    29461 tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc
    29521 aataaccgtg aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt
    29581 aagcaataaa aaaggcagca cgcccggcaa cacaatgcct gaatgaaggc tgcataattc
    29641 gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt
    29701 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
    29761 gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag
    29821 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
    29881 gcatccttat gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
    29941 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt
    30001 tacccttcgt gcgaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
    30061 cacaaaatgc cgttgacaag gttgaagaga ttaaacagtc cgttgccccc gttcagccag
    30121 gcatgtttgc tgatttcaat accgacggta aagatcagca tttgcagcat ccccatcgcc
    30181 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
    30241 ttcgccagac caataccgaa agcataaata cttaacccgg cggtcatcca taaatacgcg
    30301 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
    30361 cccataataa tcagcgaacg tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta
    30421 cccgcaatta acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac
    30481 tgctcaccgg taataatgat aatcggcgac tgggcgatcc acgccaacaa cggcaggcta
    30541 acgaatccca gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag
    30601 tcacgaccga gttctttcag cgacagtttc tcgcctatac gcgtggcggt ttcaggcata
    30661 gctcgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaagac aaacatccct
    30721 tcccagggca gcacatggat ccacgccgcg cccaccagcg gaccaagtag tggagcaatc
    30781 agcgccacgt tcgccattag cgcggtgatc ttgatacaaa cggcctcttc gaaggattcc
    30841 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttataccctg caagaagcgc
    30901 aacagggtga attgttcaat gttttgtgcc agtaatattg ccagacaggt gacgataaac
    30961 cacaccactc ccgccagcat caccggacgg cggccaatac gatccgacaa cggccccagg
    31021 agccattgta aaaacatccc gcccgccaga tacgcagtca tcgaagtagg aacccaatca
    31081 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg
    31141 atataggttg aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca
    31201 agcctgacac cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc
    31261 aaagtttaat aaaagcgcag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa
    31321 gcacattctt acattattgc gagtatagct acgctttctt taagttttat ttaacctttg
    31381 cccgttacaa tcacctaccg taaacaggcc gcttgaggga aataagacga tgccgcttta
    31441 cccagtttaa cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac
    31501 tttgcgtcgg gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc
    31561 aacgccagac tctgcgccgt ggatgatctc gttggcgatt tttattgcta aagatttgat
    31621 taccgtggtg ccgttgctgg ccgcagtact ttggttgtgg gggcttacag cacaacggca
    31681 actggtgata aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg
    31741 acatcttttt ccgcacgatc gaccctttgt cgaaaatatc ggctataact tcctgcatca
    31801 tgcggcggat gactcattcc cgagcgatca cggtacggtg attttcacct ttgcactggc
    31861 atttttatgc tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat
    31921 tgcctggtcg cgcgtttatc ttggcgtcca ctggccgctg gatatgctcg gtggattgct
    31981 ggcaggtatg attggctgcc ttagcgccca gattatctgg caagcgatgg ggcataaact
    32041 ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg
    32101 ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt
    32161 cagagggatt atggaaacac gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa
    32221 acgcagtgat aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac
    32281 gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt
    32341 tctggaacca cgcagcgcca gccattacct gttaagcgat caaaaatccc gcctggtgga
    32401 agaaaaacgc cgggcggcaa aactggctgc gacgctgata gaacccgatc agaccctctt
    32461 ttttgactgt ggcaccacca cgccgtggat tattgaagca attgataatg aaatcccttt
    32521 taccgccgtt tgttattcgc taaatacttt tctggcgctg aaagagaaac cccattgccg
    32581 cgcgtttctt tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt
    32641 tcagcaaacg ctgaataatt tttgcccgga tatcgctttt tattccgctg cgggcgtgca
    32701 tgtcagtaaa ggcgctacct gctttaatct tgaagagttg ccggtaaaac actgggccat
    32761 gtcgatggcg caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc
    32821 ggcgcgcatg ggtgacctga aacgctttga tattgttgtg agcgattgtt gcccggaaga
    32881 tgagtatgtg aagtacgcgc agacgcagcg tattaagttg atgtattaat gacgtataac
    32941 cggatgacgt ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac
    33001 tgatggaatt tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca
    33061 ttttccatca cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg
    33121 gtgccgacaa cctgaccttt tttcagcggt gcggtaagct gcggttccat taacgtataa
    33181 ctcgccttca ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc
    33241 ccgagattca cttcgctctt atcaccaaac cagacgcgct gagtcacaaa tgtggcatca
    33301 ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac
    33361 tcattaaaac ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg
    33421 ccctgggtag ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg
    33481 ccatcaacat tcagattgct gctccacagc agacgattac ggttaggctg acgaattttg
    33541 ttgaaggtga actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat
    33601 gctttaccca gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccaggccg
    33661 tgcaccgtct ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa
    33721 ccaataaatg actcctggct cccggcaacg taatcagcca gcgcaataca ggcgtcatta
    33781 ccggactgga taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg
    33841 aggaacatta ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg
    33901 accgtcacca tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag
    33961 ctggtcatga ttttagtcag gctcgcggga tccagtttct catccgcatt gccttctgcc
    34021 agcactttac cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct
    34081 tcaacggttt gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc
    34141 gctgcaagac cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt
    34201 caaaaaaaat acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa
    34261 cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gctgcttcgc
    34321 tgcttctggt gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg
    34381 gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg
    34441 gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac
    34501 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
    34561 cgatgaaccc taacgggctg gtgccattgt tgcgtgacga cgaaagtgat ctcattcttt
    34621 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgcctgtgga
    34681 tcgactcacc ggcacgtcgt gcggaagcag aaaaatggat ggactgggca aaccagacgc
    34741 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
    34801 atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg
    34861 cggaactggc aaaagtaaaa tggttctcag gcgacgagtt tggtgtgggc gatatcgcta
    34921 tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc
    34981 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
    35041 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
    35101 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
    35161 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
    35221 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttgtcg ctgttataga
    35281 aggccatgcc gctcacggcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
    35341 cgacgatctc ccctttcgct tccggtatct taaagcccga atagttgatt ccccaggttg
    35401 ccagcggcca gccgtagttt ttgccttttt gcggaatatt aatttcatca ccaccgcgcg
    35461 ggccatgttc attcagccat agtgcattac tccacggatt catcgccatt ccttgcggat
    35521 tacgaatgcc ataagaccag atctcggcgc gcacaccaga ttcctttata aaaggattat
    35581 catccgggat ttctcccagg tcggtcagac gcaccagttt gccctgtaat ttatccagat
    35641 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
    35701 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcgaaaaa
    35761 cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
    35821 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgcgactgag
    35881 caaaatcagg cgctaaaacc acgtccagca gaccgccctg cccgtgcgcc caaacgtccg
    35941 gcactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
    36001 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagcgcc cagggatggt
    36061 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agctgcccag agagcggaag
    36121 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
    36181 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
    36241 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
    36301 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
    36361 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
    36421 taatgcttct tcagcatgat gaagagcact ccattcttcc tgatccagat gaagattcaa
    36481 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
    36541 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cggcgaggtc
    36601 aacgcgtgcg tcgatcaacc ggttcagcat atcgattcgc tgtctgtcaa cgaacatact
    36661 tcgatcctcc tctttccagc cttgttgcaa ctaaatagta tggctgtttt ttgagcaaaa
    36721 tgctgtccag gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcaactccg
    36781 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
    36841 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
    36901 gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac
    36961 tacgcactga aggttatgac gtggtaccga gctatgatga tgcggacatg gtgatcgtca
    37021 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
    37081 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
    37141 gcgaagtcca cccgaaagtg ctggaaatca ctgggcctca tagctatgag caggttctgg
    37201 agcacgttca tcactacgta ccaaaaccga aacacaaccc atttctgagc ctggtgccag
    37261 aacaaggtgt gaaactgacg ccgcgtcatt atgcctatct gaaaatttct gaaggctgta
    37321 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgatctggtg agccgtccga
    37381 ttggcgaagt gttaagtgaa gcgaaacgtc tggttgatgc gggcgttaaa gaaattctgg
    37441 tgatctcgca ggatacttcc gcgtatggcg tcgatgttaa acatcgtact ggcttccaca
    37501 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
    37561 tctggacacg tctgcactat gtttaccctt atccgcatgt ggacgacgtc atcccactga
    37621 tggcagaggg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
    37681 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
    37741 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgttggc ttccctggcg
    37801 agacggaaga agatttccag atgctactcg acttcctgaa agaagcacgt ctggatcgcg
    37861 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
    37921 ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagttgcag cagcaaattt
    37981 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
    38041 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
    38101 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgagtgaaa gtcgagcacg
    38161 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
    38221 tgtccggcct gcgaataatg attactgcca gctaatctgc gccctgccgt ttttcttact
    38281 cttatacagc cgctcatccg ccagcatttt caaacgttca acggtagtac attcatttga
    38341 atgcgctaca ccgatactgg tgcttaaatt aatgcttccc acctggaaat gaacgatttc
    38401 ttttagaatc gatagcaact cttccagcgt gccgccagta taaatcaccg caaattcgtc
    38461 acctccgaag cggtagactt tacctttatc taccgggatc tgtgatttaa ggattttcac
    38521 cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa
    38581 tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc
    38641 gtcaaaagat tttctgccca tcgctttggt cagatcgtcc aggaatgccg agttatacat
    38701 actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc
    38761 aaaatggtta ttaataatgg cttctttctc cacaatacca cgattaatca ctctgcgtac
    38821 cagatagagg caactggcac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa
    38881 tgtacatacc tgaaaataaa cgtctttttt ataagcaaat acgtacaacc cggtcggcaa
    38941 ataggagaag tgctccattg cttccgaaac ggtgaagatg gcgtttttag cgttatattc
    39001 atcaccggtc accgatttac cgttcttgaa cagggagaac atcaggttct cgctatccat
    39061 cgccagcgat tttgacagta aagaaaaacg ttccagacca agcaaaaata tcagactacg
    39121 attgtgagtt accggataga ggtaatagac gaaggtttta ccatcaaaag agataaaccc
    39181 tgtatttttg acatgtcgat acttaacaac ctgcataata tctgcacaat aatttttctc
    39241 gaactccttt acctgaatac attcatcact ggaaatcaga taaggaccga catcgtacaa
    39301 ataagagggt gaaaccgagt cggcgtaagc tgtgcgtaag ccaaccaaca gctgttcctc
    39361 cgtaggattt agcgtgttga tgagatcaat tgccacacta cgccgatatt cagcaatatt
    39421 tgccaccgca aaagcagtat caataacgga ctgtttcagg ccatacaccc gttcctttac
    39481 catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac
    39541 tgtaagtacg aatttattga ttctggacat acgtaaacta ctcttttact aattttccac
    39601 ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac
    39661 atcctaattt gattaattca cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg
    39721 ggatccggtt atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga
    39781 aaatatcgcc ggtaaattgc gaatccacct ttatactgtc tggattaatt tcataaaaac
    39841 gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tacccgtacc ccttgtttac
    39901 gcagttgttc aatattgaga tagaaatgtt cattaattcg ctcgatcacg gtttcggtga
    39961 tctcaacaca aaggctgttc gccttcaggc cattttcttt catgatggta gtaaaacgct
    40021 catgaaagtg tggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat
    40081 ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc catcgcggtt ttaataattt
    40141 gatagcccag ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg
    40201 aaataatccc ccgctccgca gacacccaac ggcacaacgc ctccgcgccc aaaatggtgc
    40261 gagtgttaat atcaacaatc ggttgataga ccaggaatag ttcgccaatt tccattgcat
    40321 ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgaatttc gcatcatcac
    40381 agaaaataaa ctctgagaac atatggttgc ggatactgga aatcgccagt ttcagctttt
    40441 ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatagcagac ttatggatgc
    40501 gcagattgcg gtagccaaaa ctgctgtcaa aatcgtttac cgtcgctatc ttacgggtaa
    40561 acgtctgtac attttcccgg cacaggagta aatagagatc ggcattaaat gtcacgatat
    40621 cgcagcattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
    40681 gttcatcaac accaaacacc gcttccacag cttccagacc acacagtttg atgatcccca
    40741 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt
    40801 cctgatcgta agagatcagc gagagcaatt tatttgcccg ttcaatttca gcatctttca
    40861 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttca cgaatcttca
    40921 gagcctcaat ttgatccgta gagtcgggca aaaacgccag cgtattgacc aaatctgtca
    40981 tttcagaaaa ttgccgacgg aacactcgag tggtcattaa ataaaccacc agcaatacga
    41041 taataatgaa agcaaacggc agataaaaaa gtttatctga tgcgttcaat aaattaccac
    41101 caggataata aaaatagatt tcgaataatg cgttgtttgg caccgctgat gttttttcaa
    41161 tatattccgt atcgacagaa tcaataatat tata
//
LOCUS       DAZKHK010000030        39683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKHK010000030 DAZKHK010000000
VERSION     DAZKHK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.030"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            464..2749
                     /gene="nrdA"
                     /locus_tag="ACHOS5_001608"
     CDS             464..2749
                     /gene="nrdA"
                     /locus_tag="ACHOS5_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9776432.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2939..4069
                     /gene="nrdB"
                     /locus_tag="ACHOS5_001609"
     CDS             2939..4069
                     /gene="nrdB"
                     /locus_tag="ACHOS5_001609"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9776433.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4069..4323
                     /gene="yfaE"
                     /locus_tag="ACHOS5_001610"
     CDS             4069..4323
                     /gene="yfaE"
                     /locus_tag="ACHOS5_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP9776434.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4377..5027)
                     /gene="inaA"
                     /locus_tag="ACHOS5_001611"
     CDS             complement(4377..5027)
                     /gene="inaA"
                     /locus_tag="ACHOS5_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP9776435.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5230..6306)
                     /gene="glpQ"
                     /locus_tag="ACHOS5_001612"
     CDS             complement(5230..6306)
                     /gene="glpQ"
                     /locus_tag="ACHOS5_001612"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9776436.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNVLEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6311..7669)
                     /gene="glpT"
                     /locus_tag="ACHOS5_001613"
     CDS             complement(6311..7669)
                     /gene="glpT"
                     /locus_tag="ACHOS5_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP9776437.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            7942..9570
                     /gene="glpA"
                     /locus_tag="ACHOS5_001614"
     CDS             7942..9570
                     /gene="glpA"
                     /locus_tag="ACHOS5_001614"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP9776438.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGINAEAIEPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9560..10819
                     /gene="glpB"
                     /locus_tag="ACHOS5_001615"
     CDS             9560..10819
                     /gene="glpB"
                     /locus_tag="ACHOS5_001615"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP9776439.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTEIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHVAQQIAQRAGGQQ"
     gene            10816..12006
                     /gene="glpC"
                     /locus_tag="ACHOS5_001616"
     CDS             10816..12006
                     /gene="glpC"
                     /locus_tag="ACHOS5_001616"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP9776440.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12199..13110
                     /gene="rpnE"
                     /locus_tag="ACHOS5_001617"
     CDS             12199..13110
                     /gene="rpnE"
                     /locus_tag="ACHOS5_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP9776441.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGLHEQAIKIAL
                     RMLEQGFEREIVLAATQLTDADIPNCH"
     gene            13111..13344
                     /locus_tag="ACHOS5_001618"
                     /pseudo
     CDS             13111..13344
                     /locus_tag="ACHOS5_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13385..14188)
                     /gene="yfaU"
                     /locus_tag="ACHOS5_001619"
     CDS             complement(13385..14188)
                     /gene="yfaU"
                     /locus_tag="ACHOS5_001619"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFP9776442.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(14206..15495)
                     /gene="yfaV"
                     /locus_tag="ACHOS5_001620"
     CDS             complement(14206..15495)
                     /gene="yfaV"
                     /locus_tag="ACHOS5_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776443.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNIAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(15552..16757)
                     /gene="rhmD"
                     /locus_tag="ACHOS5_001621"
     CDS             complement(15552..16757)
                     /gene="rhmD"
                     /locus_tag="ACHOS5_001621"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFP9776444.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYREYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYAIKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(16772..17554)
                     /gene="yfaX"
                     /locus_tag="ACHOS5_001622"
     CDS             complement(16772..17554)
                     /gene="yfaX"
                     /locus_tag="ACHOS5_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9776445.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWERATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(17774..18976)
                     /gene="yfaY"
                     /locus_tag="ACHOS5_001623"
     CDS             complement(17774..18976)
                     /gene="yfaY"
                     /locus_tag="ACHOS5_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP9776446.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRADAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(19076..19618)
                     /gene="yfaZ"
                     /locus_tag="ACHOS5_001624"
     CDS             complement(19076..19618)
                     /gene="yfaZ"
                     /locus_tag="ACHOS5_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP9776447.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            19897..20322
                     /gene="nudI"
                     /locus_tag="ACHOS5_001625"
     CDS             19897..20322
                     /gene="nudI"
                     /locus_tag="ACHOS5_001625"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9776448.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(20361..20963)
                     /gene="ais"
                     /locus_tag="ACHOS5_001626"
     CDS             complement(20361..20963)
                     /gene="ais"
                     /locus_tag="ACHOS5_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP9776449.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            21271..22410
                     /gene="arnB"
                     /locus_tag="ACHOS5_001627"
     CDS             21271..22410
                     /gene="arnB"
                     /locus_tag="ACHOS5_001627"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP9776450.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALKQLAGQ"
     gene            22414..23382
                     /gene="arnC"
                     /locus_tag="ACHOS5_001628"
     CDS             22414..23382
                     /gene="arnC"
                     /locus_tag="ACHOS5_001628"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP9776451.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            23382..25364
                     /gene="arnA"
                     /locus_tag="ACHOS5_001629"
     CDS             23382..25364
                     /gene="arnA"
                     /locus_tag="ACHOS5_001629"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP9776452.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            25361..26251
                     /gene="arnD"
                     /locus_tag="ACHOS5_001630"
     CDS             25361..26251
                     /gene="arnD"
                     /locus_tag="ACHOS5_001630"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP9776453.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            26251..27903
                     /gene="arnT"
                     /locus_tag="ACHOS5_001631"
     CDS             26251..27903
                     /gene="arnT"
                     /locus_tag="ACHOS5_001631"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP9776454.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVVIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            27900..28235
                     /gene="arnE"
                     /locus_tag="ACHOS5_001632"
     CDS             27900..28235
                     /gene="arnE"
                     /locus_tag="ACHOS5_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP9776455.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGLAFIIG
                     GIVILGSTV"
     gene            28235..28621
                     /gene="arnF"
                     /locus_tag="ACHOS5_001633"
     CDS             28235..28621
                     /gene="arnF"
                     /locus_tag="ACHOS5_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP9776456.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(28615..28881)
                     /gene="pmrD"
                     /locus_tag="ACHOS5_001634"
     CDS             complement(28615..28881)
                     /gene="pmrD"
                     /locus_tag="ACHOS5_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP9776457.1"
                     /translation="MEWLVKKTCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(28991..30346)
                     /gene="menE"
                     /locus_tag="ACHOS5_001635"
     CDS             complement(28991..30346)
                     /gene="menE"
                     /locus_tag="ACHOS5_001635"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP9776458.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIHLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     VSAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(30343..31305)
                     /gene="menC"
                     /locus_tag="ACHOS5_001636"
     CDS             complement(30343..31305)
                     /gene="menC"
                     /locus_tag="ACHOS5_001636"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP9776459.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQESWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(31305..32162)
                     /gene="menB"
                     /locus_tag="ACHOS5_001637"
     CDS             complement(31305..32162)
                     /gene="menB"
                     /locus_tag="ACHOS5_001637"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP9776460.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(32177..32935)
                     /gene="menH"
                     /locus_tag="ACHOS5_001638"
     CDS             complement(32177..32935)
                     /gene="menH"
                     /locus_tag="ACHOS5_001638"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP9776461.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(32932..34602)
                     /gene="menD"
                     /locus_tag="ACHOS5_001639"
     CDS             complement(32932..34602)
                     /gene="menD"
                     /locus_tag="ACHOS5_001639"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP9776462.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCFEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(34691..35986)
                     /gene="menF"
                     /locus_tag="ACHOS5_001640"
     CDS             complement(34691..35986)
                     /gene="menF"
                     /locus_tag="ACHOS5_001640"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP9776463.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEINNKAAGLRTLLQME"
     gene            complement(36065..36370)
                     /gene="elaB"
                     /locus_tag="ACHOS5_001641"
     CDS             complement(36065..36370)
                     /gene="elaB"
                     /locus_tag="ACHOS5_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP9776464.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(36425..36886)
                     /gene="elaA"
                     /locus_tag="ACHOS5_001642"
     CDS             complement(36425..36886)
                     /gene="elaA"
                     /locus_tag="ACHOS5_001642"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9776465.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGDNRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            36951..37868
                     /gene="rbn"
                     /locus_tag="ACHOS5_001643"
     CDS             36951..37868
                     /gene="rbn"
                     /locus_tag="ACHOS5_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP9776466.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(37989..>39683)
                     /gene="yfbK"
                     /locus_tag="ACHOS5_001644"
     CDS             complement(37989..>39683)
                     /gene="yfbK"
                     /locus_tag="ACHOS5_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFP9776467.1"
                     /translation="SGCGPEPEDKKSQQQQSTTPTDQQVLAAQQAATKVAEQSAAKAA
                     ADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQIANPGTARYQQFDDNPV
                     KQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEEIVNYFPSDWDIKDKQS
                     IPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELPASNLVFLIDTSGSMIS
                     DERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAIDSLDAE
                     GSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQRESGV
                     TLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVAKDVKA
                     QIEFNPAWVTEYRQIGYEKRQLKAEDFNNDNVDAGDIGAGKHITLLFELTLKGQKASI
                     DKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPLASAIKAPSEDMRFRAA
                     VAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFIRLIGLAKDLDNSQN"
BASE COUNT         9646 a        10631 c         9881 g         9525 t
ORIGIN      
        1 aaaagtaaca aattggctta gtatttaaca ataaccgaat ataaaacaac catttccgac
       61 tcaacaatcg cttacactcg cttatatatt gaccacaact gatacatcag attatgtgac
      121 gacttgtgcc tagatcaatt ttggcaatca ttagcaacaa gattaataaa ccatctatat
      181 caatttacct atcctatttt accgttcaag aattcaccga acggttattt ttaacaaatt
      241 tttctcttcc cattgacttt cccggacacc ttgtctgacc taaggtgcgc ggaagccact
      301 ttttccttcc tgagttatcc acaaagttat gcacttgcaa gagggccagt ttcacactat
      361 cttgcagtga atcccaaaca taccccctat atatagtgtt cttagcagct tccctcacta
      421 caggtagtct gcatgaagct attgaaaaac aggtacgaca tacatgaatc agaatctgct
      481 ggtgacaaag cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct
      541 ggattgggcg gcagaaggac tgcataacgt ttcgatttcc caggtcgagc tgcgctccca
      601 cattcagttt tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc
      661 cgcagacctg atctcccgtg atgcgccgga ttatcagtat ctcgccgcac gtctggcgat
      721 cttccacctg cgtaaaaaag cctacggtca gtttgagccg cctgcgctgt acgaccatgt
      781 ggtgaaaatg gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga
      841 agagttcaag cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc
      901 tgccgttaag cagctggaag gcaaatatct ggtacagaac cgcgtgaccg gcgaaatcta
      961 cgaaagcgcc cagttccttt atattctggt tgccgcgtgc ctgttctcga actacccgcg
     1021 tgaaacgcgc ctgcaatacg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc
     1081 gctgccgacg ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcatgcgt
     1141 gctgatcgag tgcggcgaca gcctggattc cattaacgcc acctccagcg cgattgttaa
     1201 atacgtttcc cagcgtgccg ggatcggcat caacgccggg cgtattcgtg cgctgggtag
     1261 cccgattcgc ggtggcgaag cgttccatac cggttgtatt ccgttctaca aacatttcca
     1321 gacagcggtg aaatcctgct ctcagggcgg tgtgcgcggc ggtgcggcaa cgctgttcta
     1381 cccgatgtgg catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggtgtgga
     1441 aggcaaccgc gtgcgtcata tggactacgg ggttcaaatc aacaaactga tgtatacccg
     1501 tctgctgaaa ggtgaagata tcactctgtt cagcccgtcc gacgtaccgg ggctgtatga
     1561 cgcgttcttc gccgatcagg aagagtttga acgcctgtat accaaatatg agaaagacga
     1621 cagcatccgc aagcaacgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg
     1681 tgcgtctacc ggtcgtatct atattcagaa cgttgaccac tgcaataccc atagcccgtt
     1741 tgatccggcc atcgcgccag tgcgccagtc taacctgtgc ctggaaatcg ccctgccgac
     1801 caaaccgctg aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc
     1861 tttcaacctg ggcgcaatta acagcctgga tgaactggaa gagctggcaa ttctggcagt
     1921 acgcgcactc gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg
     1981 agcgatgggt cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa
     2041 gcacggtaaa cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc
     2101 cattcagtat tacctgctga aagcgtctaa tgagctggcg aaagagcaag gcgcgtgccc
     2161 gtggtttaac gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga
     2221 tctggatgcc atcgctaatg agccgctgca ttacgactgg gaagcactgc gtgagtcaat
     2281 caaaacgcac ggcctgcgta actccacgct ttctgccctg atgccgtccg agacttcttc
     2341 gcagatctct aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc
     2401 gtcgaaagac ggtattctgc gccaggtggt gccggactac gagcatctgc acgacgccta
     2461 tgagctgctg tgggaaatgc cgggtaacga tggctacctg caactggtgg gtatcatgca
     2521 gaaatttatc gatcagtcga tctctgctaa caccaactac gatccgtcac gtttcccgtc
     2581 aggaaaagtg ccgatgcagc agttgctgaa agacctgctc actgcctaca aattcggggt
     2641 caaaacgctg tattatcaga acacccgtga cggcgcagaa gacgcgcagg acgatctggt
     2701 gccgtcaatc caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgtgatgc
     2761 cggatgcggc gtgagcgccc tacccggcct acgggtccaa tttgtaggcc ggataagcga
     2821 agcgcatccg gcactggatg cccagatgcg gcatgaacgc cctacccagc ctacgatgcg
     2881 gtttgtaggc cggataagcg aagcgcatcc ggcataaaac tcctaacagg acacattcat
     2941 ggcatatacc accttttcac agacgaaaaa tgatcagctc aaagaaccaa tgttctttgg
     3001 tcagccggtc aacgtggctc gctacgatca gcaaaaatat gacatcttcg aaaagctgat
     3061 cgaaaagcag ctctctttct tctggcgtcc ggaagaagtt gacgtctccc gcgaccgtat
     3121 cgattaccag gcactgcctg aacacgaaaa acacatcttt atcagcaatc tgaaatatca
     3181 gacgctgctg gattccattc agggtcgcag tccgaacgtg gcactgctgc cgctgatctc
     3241 gatcccggaa ctggaaacct gggtagaaac ctgggcgttc tcagaaacga ttcattcccg
     3301 ctcctatacc catatcatcc gtaatatcgt taacgatccg tctgttgtct ttgacgatat
     3361 cgttaccaac gagcagatcc agaaacgtgc ggaggggatc tccagctatt acgatgaact
     3421 gatcgaaatg accagctact ggcatctgct gggcgaaggc acccacaccg ttaacggtaa
     3481 aaccgtaacc gttagcctgc gcgagctgaa gaaaaaactg tatctctgcc tgatgagcgt
     3541 taacgcgctg gaagcgatcc gcttctacgt cagctttgcc tgctctttcg cattcgctga
     3601 acgtgagctg atggaaggta acgccaaaat tattcgcctg attgcccgcg acgaagccct
     3661 gcacctgacc ggcacccagc atatgctgaa tctgctgcgc agcggcgcgg atgatccgga
     3721 gatggcggaa attgccgaag aatgtaagca ggagtgctat gacctgtttg tccaggcggc
     3781 acagcaagag aaagactggg cggattatct gttccgtgat ggttcgatga ttggtctgaa
     3841 taaagacatt ctctgccagt acgttgaata catcaccaat atccgtatgc aggcagtcgg
     3901 tttggatctg ccgttccaga cgcgctccaa cccgatcccg tggatcaaca cctggctggt
     3961 gtctgataac gtgcaggttg ctccgcagga agtggaagtc agttcttatc tggtcgggca
     4021 gattgacgcg gaagtggaca ccgacgattt gagtaacttc cagctctgat ggcccgcgtt
     4081 accctgcgca ttactggcac acaactgctg tgccaggatg aacacccttc ccttctggcg
     4141 gcgctggaat cccacaatgt ggcggttgag taccagtgcc gcgaaggtta ctgcggctcc
     4201 tgtcgcaccc gactggtcgc aggccaggtg gactggattg ccgaaccgtt agcttttatt
     4261 cagccggggg aaattttgcc ctgttgttgc cgggcaaaag gcgatattga aatcgagatg
     4321 tgaattggtt ttaacgccag atacgacgct tatacgtctt atctggccaa caacgattac
     4381 atagcgtagt aatatgcttt cacctgctcc cagtccgctt tggggatcgg ctccagatat
     4441 ttttccagct ggcggaagtc atgattaatc gctttatcac gacgcaagcg gcgacgactt
     4501 ttctccagat caagaaaacc agcttctgcc ttaccttctg ttttcacata gatatggcga
     4561 acataacaac agccatgctg acgattaacg ctatgcattt tcttaaacgc cagcgccact
     4621 gctttcaaca tggcttgccg tacttcgtca gaataaggcg atactgcatg ccgggcatac
     4681 cagtcagcaa tgctgatgaa ccccgccata tcttcagtca ccagcaacgc gcgccattca
     4741 ccctcaattt tcaccgcttc accaaaaacg atcttcggca cgatgacacc ggcccgttcc
     4801 agttctttaa ttaccgcaac ctcacggaca atcgttggtc ggccgaacgg ataacgtacg
     4861 gaatgaaaca gatgatgcgt catgcgcttt acatacagct ttttgccgtt gcgctcgacg
     4921 cattgcaccc cgctcatacc attacggcga tagttaggct cttcaaccca gtcgccctct
     4981 gttgcccacc agtgattaaa ttcgtcgtgt tttgctgaaa ctgccatacc ctatcgcctg
     5041 tcgtttttta ttaacgacaa tgactataga tgtttagctg aggaaaatct taataatacg
     5101 tgttgtattg acgagtatct tatgccggga acaagtcatc tcgctcaaaa taattgcaat
     5161 taaggataac ccacagactt taatgaagag tggtatcgga tagcacataa gtgccatccg
     5221 gtagttaatt tactctttat taaggaattt tactgcctta tcagggaaat cagtaaatag
     5281 cccatttaca cccgctttgt tatacagagc atcatataac tgattcacat cagtagtgta
     5341 ttcaggcagt ttatctgacc gcacggtata aggatgcact accagtttat tctgctgagc
     5401 atcttgcacc atgccagtga gtttgatatt acccggctgc gatgtctcct caatcaacat
     5461 atggtaatcc ggaccaatac catcagcata ttccgccacc tgtttcatag cccccggctt
     5521 aaacatccag tcgtaattgt aattaaccca gcttccgtcc ggctgtttct gctgcgtttc
     5581 attccagtcg gtataggcaa tcagctgtac cagattgaga tccataccca ttttgggttc
     5641 cagcacattc ttaatacgct tcagctcatc agcatcaaaa cattgcaaat aaactttgtc
     5701 gtccttaccg gtgtaaccat atttcttcag cacttccagc gtttttgccg caatatcctt
     5761 cccttcctga tgatggaacc acggcgcttt aatttctgga tagataccga tatttttccc
     5821 ggtagagtgg tttaacccct gaacaaattc aatctcttct tcaaaggtgt gcacccggaa
     5881 gtcagattta cccattggga aacgccccgg ataagtctgc acttttttac cgttttcaat
     5941 atcgaaacct tcggtaaatt tcagcgactt aatttcatcc agcgtgaaat ctatcgcgta
     6001 gtaacgaccg tctttgcgcg cccgatccgg gaaacgatcg gcaacatcag taacacgatc
     6061 gagataatgg tcatgcagaa caaccagatg gtcgtctttg gtcatcacca aatcctgttc
     6121 cagataatcc gctccctgcg catacgccat cgcttttgct ggcagcgtat gctccggcaa
     6181 atatccactg gcaccgcgat gggcgatgac tattttttcg ttgctgtccg ccgccattgc
     6241 actgcttccc atgactatag tgctcatcat gatcgccatg ctaaggtttt tcagcgtcaa
     6301 tttcatgcca ttagcctccg ttgcgttttt gcagtaattg ttcatggcga cgtttttcgc
     6361 caatcatcac aacaatcaac aagataaccg ccagaatgct tccgccaatc attaccataa
     6421 agccgccatc ccagccgaag aagtccacgg tgtagccaac aatcgcgctc gccgccaccg
     6481 aaccgccgag gtaaccaaac agcccggtaa aacccgctgc cgtacctgcc gcttttttcg
     6541 gtgccagttc cagcgcatgt agaccgatca gcatcacagg gccatagatc aggaagccga
     6601 taacaatcat acaaatcata tcgacggttg ggttacctgc cgggttcatc cagtaaacga
     6661 tagtcgcgat ggtcaccagt gtcataaaga agacgccggt tgccccacgg ttaccacgaa
     6721 agactttatc cgacatccag ccgcacagca gggtgcccgg aatacctgca tattcataaa
     6781 ggaagtaggc ccaggaggat ttatccagcg cgaaatgctt aacctctttc agataagtcg
     6841 gtgaccagtc gaggatgccg taacgcagca gataaacgaa cacgttggca atggcgatgt
     6901 accacagcag tttgttcggc agcacgtact gcatgaagat ctgcttcgcg gtcagctcct
     6961 gttccgcttt ttcgttatag tcgtccggat aatcattttt gtattcttcg atcggcggca
     7021 ggccacagga ttgcggggta tcgcgcatca tcgcaaaggc gaacagtgcc accagaatgg
     7081 cgcagaaagc gggcatatag agcgccgcgt gccagtcatt gaaccaggcc atccccagca
     7141 ggaacagcag cggcggaata ccaccaccga cgttgtgcgc acagttccac actgacacaa
     7201 tgccgccacg ttctttctgc gaccaccagt gcaccatagt acgaccacac ggcggccacc
     7261 ccatcccctg gaaccaaccg cagaggaaca acagtacaaa catcaccgca atgctcgacg
     7321 tcgcccatgg cacaaagccc ataaacaaca tcactgccgc cgccagaatc aaacctgcgg
     7381 gcaggaaaac gcgcggattc gagcgatccg ataccgaacc catgatgaat ttcgaaaatc
     7441 cataagcaat cgagatcccc gaaagggcaa aacctaaatc accgcgtgag aatccctgct
     7501 caaccagata aggcatagcg agcgcaaagt tcttacgaac caaatagtaa gcagcatagc
     7561 caaagaatat ccccaggaaa atttgccagc gcaatcgacg ataagtcgga tcgatctccg
     7621 cggcaggtaa gcgcgctttg tgtggcgctg gtttaaaaat actcaacatt gatagcctcc
     7681 gtggcccgtg gtcttattta tgattaacag cctgattcag tgggagaacc tgccgtttct
     7741 tgagttgccg cgatgttaag aaaacattca taaattaaat gtgaattacc gcacacatta
     7801 ttaaatacga tggttgaaat gttcagaatg acgcatgaaa tcacgtttca ctttcgaatt
     7861 atgagcgaat atgcgcgaaa tcaaacaatt catgttttta ctatggctaa atggtaaaaa
     7921 acgaacttca gagggataac aatgaaaact cgcgactcgc aatcaagtga cgtgattatc
     7981 attggcggtg gcgcaacggg agccgggatt gcccgcgact gtgccctgcg cgggctgcgc
     8041 gtgattttgg ttgagcgcca cgacatcgca accggtgcca ccgggcgtaa ccacggcctg
     8101 ctgcacagcg gtgcgcgcta tgcggtaacc gatgcggaat cggcccgcga atgtattagt
     8161 gaaaaccaga ttctgaaacg cattgcacgt cactgcgtag aaccaaccaa cggcctgttt
     8221 atcaccctgc cggaagatga cctctccttc caggccactt ttatccgcgc ctgcgaagaa
     8281 gcagggatca acgcagaagc cattgagccg cagcaagcgc gcattatcga acctgccgtt
     8341 aacccggcac tgattggcgc ggtgaaagtt ccggatggca ccgttgatcc atttcgtctg
     8401 acagcagcaa acatgctgga tgccaaagaa cacggtgccg ttatccttac cgctcatgaa
     8461 gtcacggggc tgattcgtga aggcgcgacg gtgtgcggtg ttcgtgtacg taaccatctc
     8521 accggcgaaa ttcaggcact tcatgcgcct gtcgtagtta atgccgccgg gatctggggg
     8581 caacacattg ccgaatatgc cgatctgcgc attcgcatgt tcccggcgaa aggatcgctg
     8641 ctgatcatgg atcaccgcat taatcagcat gtgatcaacc gctgccgcaa accttccgac
     8701 gccgatattc tggtgcctgg cgataccatt tcgctgattg gcaccacctc tttacgtatt
     8761 gattacaacg agattgacga taatcgagtg acggcagaag aagttgatat tctgctgcgt
     8821 gaaggggaaa aactggcccc cgtgatggcg aaaacgcgca ttttgcgggc ctactctggc
     8881 gtacgcccgc tggttgccag cgatgacgat ccgagcgggc gtaacgtcag ccgtggcatc
     8941 gtgctgctcg accatgctga acgcgatggt ctggacgggt ttatcaccat caccggtggc
     9001 aaactgatga cctatcggct gatggcggaa tgggctaccg acgccgtatg ccgcaaactg
     9061 ggaaacacgc gcccctgtac gaccgccgat ctggcacttc ctggttcaca ggaacccgct
     9121 gaagttacct tgcgtaaagt catctccctg cctgccccgc tgcgcggttc tgcggtttat
     9181 cgtcatggcg atcgcacgcc tgcctggctg agcgaaggcc gtctgcaccg tagcctggta
     9241 tgtgagtgcg aagcggtaac tgcgggtgaa gtgcagtacg cggtagaaaa tttaaacgtt
     9301 aatagcctgc tggatttacg ccgtcgtacc cgtgtgggga tgggcacctg ccagggcgag
     9361 ctctgcgcct gccgcgctgc cggactgctg caacgtttta acgtcacgac gtccgcgcaa
     9421 tctatcgagc aactttccac cttcctaaac gaacgctgga aaggcgtgca acccatcgcc
     9481 tggggagatg cactgcgcga aagcgaattt acccgctggg tttatcaggg attgtgtggc
     9541 ctggagaagg agcagaaaga tgcgctttga tactgtcatt atgggcggcg gcctcgccgg
     9601 attactctgt ggcctgcaac tgcaaaaaca cggcctgcgc tgtgccattg tcactcgtgg
     9661 tcaaagcgcg ctgcatttct catctggatc gctggatttg ctgagccatc tgccggatgg
     9721 tcaaccggtg acagaaattc acagtggact ggaatctttg cgtcagcagg cacctgccca
     9781 tccttacact cttctcgggc cacaacgcgt gctcgatctc gcttgccagg cgcaggcatt
     9841 aatcgctgaa agcggtgcgc aattgcaggg cagcgtagag ctggctcacc agcggattac
     9901 accgctaggc actctgcgct caacctggct aagttcgcca gaagtccctg tctggccgct
     9961 gcccgcgaag aaaatatgtg tagtgggaat tagcggcctg atggattttc aggcgcatct
    10021 tgcggcggct tcattgcgcg aattggatct taaagttgaa accgctgaaa ttgaactgcc
    10081 ggaactggat gtgctgcgca ataacgccac cgagttccgg gcggtgaata ttgcccgctt
    10141 ccttgataat gaagaaaact ggccgttgtt acttgatgca cttattccgg tcgccaatac
    10201 ctgcgaaatg atcctgatgc ccgcctgctt cggcctggcc gatgacaaac tgtggcattg
    10261 gttgaatgaa aaactacctt gttcattgat gcttttgcca acgctgccgc cttccgtgct
    10321 gggcattcgt ctgcaaaacc agttacagcg ccagtttgtg cgccagggtg gcgtgtggat
    10381 gccgggcgat gaagtgaaaa aagtgacctg taaaaatggt gtagtgaatg aaatctggac
    10441 gcgcaatcac gccgatatcc cgctgcgccc acgatttgcg gtgcttgcga gcggcagttt
    10501 ctttagcggc ggactggtgg cagaacgtga cggcattcgc gagccgattc tcggccttga
    10561 tgtgctacaa accgctacgc ggggtgaatg gtataagggc gatttttttg cgccgcagcc
    10621 gtggcagcag ttcggtgtaa ccactgatga ggcgctacgc ccgtcgcagg cggggcaaac
    10681 cattgagaac ctgtttgcca tcggttcggt gctgggcgga tttgatccca tcgcccaggg
    10741 atgcggcggc ggtgtttgtg ccgtcagtgc tttacatgtc gctcaacaga ttgcccaacg
    10801 cgcaggaggc caacaatgaa tgacaccagc ttcgaaaact gcattaagtg caccgtctgc
    10861 accaccgcct gcccggtgag ccgggtgaat ccgggttatc cggggccaaa acaagccggg
    10921 ccggatggcg agcgtctgcg tttaaaagat ggcgcactgt atgacgaggc gctgaaatat
    10981 tgcatcaact gcaaacgttg tgaagtcgcc tgcccgtccg atgtgaagat tggcgatatt
    11041 atccagcgcg cgcgggcgaa atatgacacc acacgcccgt cgctgcgtaa ttttgtgttg
    11101 agtcataccg acctgatggg tagcgtttcc acgccgttcg cccccatcgt caacactgct
    11161 acctcgctga aaccggtgcg gcagttgctt gatgcggcgt taaaaatcga ccaccgtcgc
    11221 acgctaccga aatactcctt cggtacgttc cgtcgctggt atcgcagcgt ggcggctcag
    11281 caagcgcagt ataaagacca ggtcgctttc tttcacggct gcttcgttaa ctacaaccat
    11341 ccgcagctag gcaaagattt aattaaagtg ctcaacgcga tgggtaccgg cgtacaactg
    11401 ctcagcaaag aaaaatgttg cggcgtaccg ctgatcgcca acggttttac cgataaagca
    11461 cgcaaacagg caattacgaa tgtagagtcg atccgcgaag ctgtgggagt aaaaggcatt
    11521 ccggtgattg ccacctcctc aacgtgtaca tttgccctgc gcgacgaata cccggaagtg
    11581 ctgaatgtcg acaacaaagg cttgcgcgat catatcgaac tggcgacccg ctggctgtgg
    11641 cgcaagctgg acgaaggcaa aacgttaccg ctgaaaccgc tgccgctgaa agtggtttat
    11701 cacactccgt gccatatgga aaaaatgggc tggacgctct acaccctgga gctgttgcgt
    11761 aaaatcccgg ggcttgagtt aacggtgctg gattcccagt gctgcggtat tgcgggaact
    11821 tacggtttca aaaaagagaa ctaccccact tcacaagcca tcggcgcacc actgttccgc
    11881 cagatagaag agagcggcgc agatctggtg gtcaccgact gcgaaacctg caaatggcag
    11941 attgagatgt ccacaagtct tcgctgcgaa catccgatta cgctgctggc acaggcgctg
    12001 gcttaaactc ctttctgatg cccggtaagc atgttgttac cgggcatttt tgcgtacacg
    12061 attccgtgcc caatgtatgc gttgcaacgc agtgaaaatt cctctgaaaa cgtctcgcaa
    12121 aggctgaaac tggcagatgt caaaggcctg ggataaccgt aatgtcgcgt catcataaat
    12181 atcaggtgac ggacaaccat gaccgaatca acaacctcct ccccgcatga tgcggtgttt
    12241 aaaaccttta tgttcacacc cgaaaccgca cgggattttc tggaaataca tctaccagag
    12301 ccgctgcgca agctttgcaa cctgcaaacc ttacgcctgg aacccactag ttttattgaa
    12361 aaaagtttac gcgcttacta ctcggatgtt ttgtggtctg tggaaaccag cgagggtgac
    12421 ggctatatct actgcgtgat tgaacatcaa agctctgcag aaaagaatat ggcttttcgg
    12481 ctaatgcgct atgccactgc cgccatgcag cgtcacctgg ataaaggcta tgacagagtt
    12541 ccgctggtgg tgccattgct gttttatcat ggcgaagcct cgccctaccc gtactcactc
    12601 aactggctgg atgagtttga cgatccgcaa cttgcccggc agttgtacac cgaagctttt
    12661 ccgttggtgg atatcaccat cgtacctgac gatgagatca tgcaacatcg gcgtatagct
    12721 ctgctggaac tgattcaaaa gcatattcgc gaccgcgatt taatcggtat ggtcgacagg
    12781 atcaccacgc ttttggttag aggcttcact aatgacagtc agctacaaac actgtttaat
    12841 tatctgctgc aatgcggcga tacctcccgt ttcacccgct ttattcagga gattgccgaa
    12901 cgttcaccac tacaaaagga gagattaatg actattgctg aacggctacg gcaggaagga
    12961 catcaaattg gctggcaaga aggtaaaatt gaaggtttgc atgagcaagc cattaaaatt
    13021 gccttgcgca tgctggagca gggctttgaa cgtgagattg tgctggcggc aacccaactc
    13081 actgatgctg atattccgaa ctgtcattaa aaggagagat tgatgactat tgctgaacgg
    13141 ctacggcagg aaggacatca aattggctgg caggaaggta agattgaagg ctggcaagaa
    13201 ggtaaattag aaggtttgca ggaaggcatg catgaacaag ccattaaaat tgctttgcgc
    13261 atgctggagc agggcattga tcgtgaccag gtgctcgcgg ccactcagct aagcgaagcc
    13321 gatctggcag cgaataacca ctaattaaca caggcccaca gccgatcccc atgggccttt
    13381 gatatcaata actacctttt acgcgtggcc catttttgcc tgatttaaac atcgccagtc
    13441 gttgatccag ggcatcgctg tagagcatcg tgtcaacgcc aacagcgaca aagttcgctc
    13501 cccacgccag gcattgctgc gccatatcag gagccacagc cagaaaacca gccgctttac
    13561 ccgcagcacg gatccgccga atgctggttt caataattcg ctgcacttcc gggtgcccgg
    13621 cgttatccgg gtagcccaac gacgcagaaa gatccgcagg tccaataaac acgccatcaa
    13681 tcccttcaac atcgaggatt tcgtccaggt tatccagtgc cgttttactt tccacctgca
    13741 ccaacagaca aagcgaatcg ttgacttgcg ccatgtaatt ctcaatgcgt ccccagcgtg
    13801 cagcccgtgc cacactggcc ccaacaccac gctcaccgta gggaggatag cgcgtggcag
    13861 acaccacctg acgtgcctga tcggcagtat cgaccatcgg gatcagtaga gtttgcgcgc
    13921 caatatccag gacttgttta atcagcggct tactgccttc caccggacgg atcacggggt
    13981 ggctggcata gggcgctacc gcctgtagct ggtgataaag atcctgaatg gtgtttggcg
    14041 catgctcccc gtcaatcagc aaccagtcat aaccggaagt ggcggcaatt tctgccatat
    14101 aggcagtcgt tgagcttaac cacagaccaa tttgcacttc gcccttgcgt aaacgttctt
    14161 taaagggatt ggttaataat gcgttcatcg tgttccttaa actatttaat gatgtgccac
    14221 gtcggtctgc gcaacagtgc gatttacacg cagagtgaaa ataatcagcg aaccgatgac
    14281 cgccaccgct gccagcgtca gtaatcccgc cgcatcgctg gaaaataacg tttctgcttt
    14341 cacgcgcagg atcggggcaa taaacccgcc cactgcacca aacaggttta taaaaccaat
    14401 ccccgcagcc agcgccgtac cggaaagaag ctgtgtcggc atcgtccaga acaccggttg
    14461 cacggcaata aacccgatgg ctgcaacaca cagcgctacg atcgccagta ctggagaaag
    14521 cagcccggac agaccaatac caatgcccgc cgccagtaat gtcagcgctg caatattacg
    14581 ccgttcgccg gttttatcgg agtagcgcgg aataagccag gtcccaaaca aggccgcaac
    14641 ccacggaatg gcggtgacca ccgacgccgt aaagcccact tttgtcccca gcaatgcagc
    14701 aacctgggtc ggcaggaaga aaattaatcc gtaaaccgcc acctgaatgg tcaggtagat
    14761 aatcgccagt tgccagactc gcccattacg caacgcatcg ctcagtcgcg aagtcacttt
    14821 ctgttgttct tcacttgcca gttgattgat aagtaacgtt ttttcttgtt tactcagaaa
    14881 acgtgcctgc tccggtgtgt catcaagcca aaagaatgtg aataccccag cgccgactgc
    14941 cagcaatcct tcaatcacaa acatccagaa ccagccggga tgccccataa atccatgcat
    15001 ctccagcagc gcgccggaaa gcggtgatcc cagtgttaac gccagcggtg cgcccatata
    15061 gaacagcccc ataatgctgg cgcgattacg ctgcggaaac cattgcgaag tgagataaat
    15121 cataccaggg aaaaaaccgg cctccgcagc accaagcaga gtgcgaacga tcagaaattt
    15181 cgcttcagta tcggcccatg ccatggcagc cgaaagaaat ccccacagca gtgttgtcgt
    15241 accaatccag gttctggccc ccagtttgcg catcaaaaga ttcgccggaa cccccagaaa
    15301 cgcgtatacc acaaagaaaa tgcctgctcc cagcgcataa gcttcattac tcagcccggt
    15361 atcaatctgg taggtctgtt tggcaaaacc aatattcgaa cggtcgagaa acgccagcac
    15421 atacagcgcc aacataaacg gaattaaacg cgcgcggttt ttcttcacca cggcgtcaag
    15481 caaagcggtg ctcataaaaa gctccttaga atatgtgggc gttaccgttg tcacagcaac
    15541 gcccggagtg attagtggct gtaggggcgt ttcaaattgc agtcacgatt gagttcgacg
    15601 ccgaaaccgg gtttatcaag cactgattta tgaatacgac cattcaccgg tactggctca
    15661 ttaagcagaa tcggatcaaa ttgcggacgc atcgttgaac aatccgggct ggtcatcagg
    15721 aattcgctga atggcgtatt ggtgaaggtg atcaccgcat ggtgggagta aacagacgaa
    15781 ccgtgtggca ccaccagttg cccccttgat ttggcgattg cggcaatttc caccagcgtg
    15841 gttaagccac cgcaccagcc aacatccggc tgcataatgt cgataccggt ttctgaaagc
    15901 gtacggaaag attgcagtgt gccgtggtgc tcaccgctgg tgaccatcat ccccgctggt
    15961 gcgttgtgtt tcagttcgcg ataaccttca tactgctgtg gcggcaggca ctcttcgatc
    16021 catttcaggt tatagggcgc gcaagcgtgg gccagtttga tggcatagtt cacgtcctga
    16081 ctcatccagc agtcgagcat taaccagaaa tcctcaccgc atttttcacg catatccgcg
    16141 accatagcgg catctttgcg gatccccgca tcaccatcat gtggccccca gtgcgtcggc
    16201 attttgccac cgataaagcc catctctttt gccagatccg gacgcgcacc tgtggcgtag
    16261 aactgaatct catcacgaac tgcgccgcct aaaagtttat aaaccggaag cccgaccact
    16321 ttgccgaaca gatcccacag agccagatcg acacaggaaa tcgtattcat caccaggcca
    16381 ccagagccgg agtaatacag ggtggcattg agcatttgat cgtggatcag tttgatatca
    16441 ctgacgcatt taccctcaat gaaacggtta agatgttttt cgacaataaa acaacccatt
    16501 tcaccggctg tcgaaacggc gaatccggtc tggccgtttt ctgcttcaac ttcaacaatc
    16561 aacgtgccaa gaacgttaat gccaaatgac tggcgggact gctcgtattc gcggtattta
    16621 ctcatcgggg tggcaatatg atcatcaatc cagtgattcg ccccctggtc gtgataatca
    16681 ccgccgccag cgcctttttc tgctgtcgca ccgccagtaa accaggcgcg aacctgtttg
    16741 atttttggta gggtcatgat gttctccatt gttatgaggc ctgtaagtca aagggacttt
    16801 tccatcccaa caaacgtgaa atatccctgg cgcaggcaat ggccttgccc gccagataat
    16861 cacggtattc ttcattgatt tgtaagcggg taccgaccac cgaaatcgca gcagtaagct
    16921 cgttattggc gttaaacacc ggcgcagcga cacaacggac atcggcgtaa tcttcgccgt
    16981 tgtcatagct ccagccctgg cggcgaatac gcgccagttc ttcgtgaagt tgctgtggat
    17041 gagtaatcgt tgtgggtgtc gcccgttccc agaccagccc ttcgataatg ctttgctgta
    17101 cagctgcagg ttgccaggca agcaggcatt tacctattcc ggagcgataa agcgaaaggc
    17161 tttttccttc atgggaacgc acgctgatag tggctgatga ctccactttc aaaatgtaat
    17221 aagcgctgcc gttatcaata atccccaggt gacataacag cccggtggta tccatcagtt
    17281 gcgtcaaacg cggtcgagcc agttcccgga gatccatctt gcttaacgca tgaccggaaa
    17341 gctccaccag cctggtccac aaacaaaaat tttcctgatg atcaaggcta agaaaacgct
    17401 gacgcctgag ttcattaagc agcaaatagg ctgtgctttt agggatcccc aatgtgtcaa
    17461 tgatcgtcgc agcactacag gggccaatcc gggcaatcaa attgagaata tcaatagccc
    17521 gtgtcagggc gggaactttg cttgattcca acatactgga ctccagtctt agatactgga
    17581 atcagtctcg ttgctcaggg ggagtaaaat tgtgaagcgc atcaaatccc gtttctcgtc
    17641 cagcggactg gagaaattac gcacaaattt gtgaatcagc gcacggattt tttcttattg
    17701 agtacaacag gttggacaaa ttttgccgga gggggaatgt gccggtgcag taaaccggca
    17761 cagagtggcg gaatcagaca gataaggtca tggactcaac aacctcaatc cagccatgct
    17821 cgctggcgat gtcctggccg tttaaccaac ggcgtagcat attaagcgcc atcattgcgc
    17881 agacttcctg gcgaatagcc agactgtagc gtgtggcact gaaatgtaca cgcaaagcga
    17941 aagtgccgtc tggcgtggct agtgcaaagt tgagatgctc atcttccaga ccagaaacag
    18001 ccagtgccaa cccagcaaaa tggttcgccc gccgttctgt aatccagtgc gcagtttgcg
    18061 ccagggtttc ctcctgtgaa ggaaccactt cacacgccag caatggagca tctgcgcgag
    18121 aaagttgcaa agccaataaa ccaccggtga attgctcgct cagcgtcagg ctgaactggc
    18181 ggctctgcaa ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc
    18241 tctgtccggc aacacgttta acgtccagcc acagtttttc catcgcctgt tcctcgcttg
    18301 ccggtccggt gagtttcagt tcgataatcg gcattgagga gcgatagccc attgttacgc
    18361 ccggcggcag ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg
    18421 tagtcaaacg caggcaaacc ggcggctgtg gtaaagaaaa acgctcgcgc aggcgcggca
    18481 ggatttcgtg ttcgaccatc accttaaatt ctgacggtac gccgggggta aagaacatca
    18541 ggcaacgatt aagctgtatg gcaaaaccac aggcagtgcc taccgggtta ttgataaatt
    18601 cagcactggc aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccccgtt
    18661 cgtggaaata gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt
    18721 ttgctgtcgc agcggcgagt gcgcttaaat catcactggt cggtcccagc ccgccgttaa
    18781 cgatcagcac atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt
    18841 tatcccccac cgtattgcgg cgagataatg gcagcccctg atgaaagaaa aaatcggcca
    18901 gccaggcagc gttagtgtca acgatttgcc cgtgtaacac ttcatccccg gtggataaca
    18961 tttccacttt taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg
    19021 agacagggcg tagtaagaaa ctgaatggcg cgacagaacg ccgcgccgga aggcgtcaga
    19081 agctggcatt taccccaaca tacgggccat cagcaatggc gttatcacga ttgccatttt
    19141 tacctgccag gttcaggtaa cggtagccag cctcaatgct gactggacgc ataatggtgt
    19201 aacgcgcgcc agcattcgct tcttcataac ttttaattcc gctggagagc gaatccggag
    19261 agtagtaata ctcaccaaac aaacggaagc tgtcaccaat ttgccactgc aaaccaccgc
    19321 caactgccgc tgcataacct tcatcaccat cgttcgggtt ggtgtaaacg ccttttccgc
    19381 caacggtcgc cattaatggc ccgagaggca gattcaaccc cagccccacg cccgcggcat
    19441 caccgtcgtc gtcgttatgt gtccagttac cgcttaacgc caggcccgtc gattcagtcc
    19501 caaaaccgac accaatattg gtgtattctt tacccgcctg gccgctgatg ctcattgcat
    19561 tgaccgatgc agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta
    19621 tcccctttcc cacaagcaat taattaaaat tcccaaagct gcgggattgt actgcctggc
    19681 gcagaggatt caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt
    19741 aaataattaa acgccgaaaa aataaacaca ctttatcgat acacacaaaa ataataataa
    19801 atgtagttat catcgcaaaa gagattactg tcactttcac atatttctga gttaccgtat
    19861 tctcatggtt tctttttatt tataaggaat acccctgtgc gacaacggac cattgtatgc
    19921 cctttgattc aaaatgatgg tgcttatttg ctgtgtaaaa tggccgacga tcgcggcgtt
    19981 ttccccggtc aatgggcgct ttcgggtggc ggcgtggagc ctggcgaacg aattgaagag
    20041 gcactacgcc gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg
    20101 ccgtggacct tcagcgatga tattcgcacc aagacgtatg cagatggtcg caaggaagag
    20161 atttatatga tttacctgat ttttgactgc gtttctgcca accgagatgt gaaaataaac
    20221 gaagagtttc aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttgaat
    20281 gttgccaccc gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca
    20341 ctggctgtca gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct
    20401 ttttccacat gcatgactaa accatccaga taatcaggtt taaatgtcgc atcacgctta
    20461 tctttagcaa tatatgtcag gcaatgatta tgggtaaaaa taacgatatt tttatcaggt
    20521 gctttgcgtt gtaagtcttt aattgcacta taaatctcat taccgcattg aagaaatcgt
    20581 ttatctaccg tcagcttttt acccgccgaa aaccaggtcg ccgactgaat agtccggacg
    20641 gtattactgg aatagagatc gaaatcaggg atgtcagcgc taaaagcgtt acccagttca
    20701 cgggcatcct gggtaccttt gaccgtaata cctgttttat ctgacaagca ttgattggtt
    20761 gaacgatcgc aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt
    20821 ctggccagtg ttttattgtc gatgcgtggc aaaccattgc tactccaggc ggcatgagta
    20881 cccagtccgg caatcgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaac
    20941 gaagatcggc aaaaagctaa cacgtaaact ccacctgtag acaagcgcaa ccagacaatt
    21001 accgtgaaat tgagcgactt ttctggcgat aattagcagt tggtgtaata ttaaaaatcc
    21061 tatgatgccg gcaaaatgcc tcaaaatttt gccaaatgca aagtctaaat aagaaaaaat
    21121 ataaaaattt caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc
    21181 cttaaatttt tgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg
    21241 gacaggtatt caatgacgga tggaaaaaaa atgtcaggat ttttgccttt ttcgcgacca
    21301 gcaatgggcg tagaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca
    21361 accggtccga agaatcaggc gcttgaacaa gctttttgcc agttgacggg aaatcagcat
    21421 gccatcgcgg tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt
    21481 ggcaagggcg atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt
    21541 tccttgctgg gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg
    21601 cctgaagcga tcgaagcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat
    21661 gccggtgcgc cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca
    21721 gttatcgaag atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca
    21781 aaaggtactg ctattttttc gtttcacgcc atcaaaaata ttacctgtgc ggaaggtggc
    21841 ctgattgtta ctgataatga aaatcttgcc cgccagctgc ggatgctgaa atttcacggt
    21901 ctgggtgtcg atgcctatga cagacatacc tggggccgtg caccgcaggc tgaagtctta
    21961 acgccgggct ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta
    22021 gtcaaattag agcacctcaa cacccgtcgg cgcgaaattg cccagcaata tcagcaagca
    22081 ctggcagctc tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg
    22141 catctgttta ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg
    22201 gaagcgttaa aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa
    22261 aaatattatc gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagtgaa
    22321 cgcatctgtt cgttgccgct gttcccggat atgaccaccg ccgatgccga ccgcgtcatc
    22381 acagccctta agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa
    22441 gtctcggtgg ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc
    22501 accaccgcag cctgtgaatc actggggaaa gagtatgaaa tcctgctgat tgatgacggc
    22561 agtagcgata attccgcgca tatgctggtc gaagcctcac aagcggaggg cagccatatt
    22621 gtgtctatct tgcttaaccg caattacggg caacattcag cgattatggc gggattcagt
    22681 cacgtcaccg gcgacttaat tattaccctc gatgccgatc tccagaatcc gccagaggaa
    22741 atcccccgtc tggtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag
    22801 aaccgccagg acagctggtt tcgtaaaacc gcttcaaaga tgattaaccg gcttattcag
    22861 cgcaccacag gcaaagcgat gggtgactat ggttgtatgc tgcgcgccta tcgccgtcat
    22921 attgtcgatg cgatgttgca ctgccatgaa cgcagcacct ttatcccgat tctggcgaat
    22981 atcttcgccc gccgtgccat tgaaattcca gtgcaccatg ccgagcgtga gtttggtgaa
    23041 tccaaataca gctttatgcg cctgattaat ttgatgtacg acctggtgac ctgtcttacc
    23101 acaacgccgc tacgtatgct gagtctgctc ggcagcatta ttgcgattgg cggttttagc
    23161 attgcggtgc tgctggtgat tttacgcctg accttcggac cccaatgggc ggcagaaggc
    23221 gtctttatgc tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga
    23281 ttgctcggtg aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt
    23341 gttcagcaag ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg
    23401 cctaccacga tatgggatgc ctcggtattg aagccctgct gtctgccggt tacgaaatta
    23461 gcgccatttt tacccatact gataaccccg gtgaaaaagc cttttatggt tcggttgccc
    23521 gactggcagc ggaaagaggc attccagttt atgcgccgga taacgttaat catccgctgt
    23581 gggtggaacg cattgcccaa ctatcaccag aggtgatttt ctctttttat tatcgccatc
    23641 ttatttgcga tgagattttg cagctcgctc cgagaggtgc atttaatctg catggttcgc
    23701 tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg
    23761 aaactggcgt tacattgcac cgaatggtga aacgtgccga tgccggggcc attgtggcgc
    23821 aactgcgcat tgccattgcg ccagacgata tcgccattac gctgcatcat aaattgtgcc
    23881 atgccgcgcg ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg
    23941 aaatcgccca gcgcgaaaac gaagccacct gttttggtcg cagaacgccg gatgacagtt
    24001 tcctcgaatg gcataagccg gcatccgtac tgcacaacat ggtgcgtgcc gttgccgatc
    24061 cgtggccggg tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg
    24121 ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat ttctgttgca ccactgctga
    24181 ttgcctgtgg cgatggcgcg ctggaaatcg tcactggaca ggcgggcgac ggcattacta
    24241 tgcagggctc gcaattagcg cagacgctgg gcctggtgca aggttcacgc ttgaatagcc
    24301 agcctgcctg tgccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta
    24361 ttggcaacca tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg
    24421 atattggcag cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgtcgaag
    24481 gcgatatcag tattcattcc gaatggattg agtatcacgt caaaaaatgt gatgtcgtct
    24541 tgcctttggt ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg
    24601 aactcgattt cgaagagaat ctgcgcatta tccgctactg cgtgaagtac cgtaagcgaa
    24661 tcatcttccc gtcgacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg
    24721 atcattctaa tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtgtcta
    24781 aacaattact tgatcgagtg atctgggcct atggcgaaaa agaaggttta cagttcaccc
    24841 tcttccgccc gtttaactgg atggggccac gactggataa ccttaatgca gcgcgaattg
    24901 gcagctcccg cgctattacg caactcattc tcaatctggt agaaggttca ccgattaagc
    24961 tgattgatgg cggaaaacaa aaacgctgct ttactgatat tcgggatggt atcgaggcgt
    25021 tataccgcat tatcgaaaat gcgggaaatc gctgcgatgg cgaaattatc aacattggca
    25081 atcctgagaa cgaagcgagc attgaagaac tgggtgagat gctgctggcg agcttcgaaa
    25141 aacatccgct gcgccattac ttcccaccgt ttgcgggctt tcgtgttgtc gaaagtagca
    25201 gctactatgg caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc
    25261 gccgctgcct ggactgggaa ccgaaaattg atatgcagga aaccatcgac gaaacgctgg
    25321 atttcttcct gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag tcggcttacg
    25381 cattgatgtc gatacctttc gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt
    25441 gagtaagcat aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg
    25501 ccatctctgg cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc
    25561 atcgctttat ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg
    25621 tcatgccaat gctgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc
    25681 ctgggatcac catgcctggc aagcccatag cggtaattgg gatcggcaaa caatgatcga
    25741 cgatattgca cgcggccttc gcaccctgga agagattatc ggtcaaccgg taacctgttc
    25801 tgccgctgct ggctggcgtg ctgaccaaca ggtgatcgaa gcaaaagaag cgttccattt
    25861 gcgctacaac agcgattgtc gtggggcgat gccgtttcgt ccattgctcg aatcaggaac
    25921 ccccggcact gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga
    25981 tgtgaaagca gaagatttta acggttggtt actcaaccgc atccagcgag ataaaggcac
    26041 gccggtttat accattcatg ccgaagttga aggctgcgct tatcagcata attttgtgga
    26101 tctcctgaaa cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactattatc
    26161 agggacgttg ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct
    26221 gggttgccaa caaatggcgg gtagtcactg atgaaatcgg tacgttacct tatcggcctc
    26281 ttcgcattta ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaacca
    26341 gatgaaacgc gttatgcgga aatcagtcgg gaaatgctgg catccggcga ctggattgtt
    26401 ccccatctgt tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc
    26461 attgggcaat ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg
    26521 accctgttaa ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt
    26581 ctggctttac tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact
    26641 tatgccgtgc tcgatccgtt tattgccttc tggctggtgg cgggaatgtg cagcttctgg
    26701 ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc
    26761 acctgcggca tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc
    26821 gtgctgccat gggtagcaac gcaaaaacgc tggaaagatc tctttattta cggctggctg
    26881 gcggttatca gttgcgtact gacggttctc ccctggggac tggcgatagc gcagcgggag
    26941 cctgacttct ggcattattt tttctgggtt gagcatattc aacgctttgc actggatgat
    27001 gcccaacata gagctccgtt ctggtactac ttgccggtcg tcattgccgg tagcctgccg
    27061 tggctgggat tactccccgg tgcactgtac gctggctgga aaaaccgcaa gcattccgca
    27121 accgtctatt tgttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggc
    27181 aaattgccca cctatattct ttcctgcttt gcacctctgg caatgctgat ggcgcattac
    27241 gcattgctgg ctgcaaaaaa taatcctctg gcgttgcgga ttaatggctg gattaatatc
    27301 gcttttggcg tcactggcat tattgccaca tttgtggtct ccccctgggg accaatgaac
    27361 acaccggtgt ggcaaacctt cgagagctat aaagtctttt gtgcctggtc tattttttcg
    27421 ctatgggcat ttttcggctg gtacacctta acaaacgtcg aaaagacctg gccttttgcc
    27481 gcgctttgcc cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg
    27541 gaaggaaaac atccgcaatt ttttgtcgag atgacacaag aatcacttca gccaagccgc
    27601 tatattctta ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat
    27661 gacatcatca tgtatcgcca gacaggtgag ttgaaatatg gccttaatta tccggatgca
    27721 aaagggagat ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag
    27781 gggattatta cactcgtgct ttcggttgac cgcgatgaag atatcaatag tctcgccatt
    27841 ccgcccgcag atgtcatcga tcgtcaggag cgtctggtgc taattcagta tcggcccaaa
    27901 tgatctggct aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga
    27961 aacaggcaac ctgctttgcg gcggtaaaca aacggcgcaa acatatcgtg ctgtggctgg
    28021 gactggcgct ggcttgtctt ggccttgcca tggtgctctg gctgctggtc ttgcagaacg
    28081 taccggtagg cattgcctac ccgatgttaa gtctgaattt tgtctgggta acgctggctg
    28141 cagtaaaact gtggcacgag ccggtatcgc tgcgtcactg gtgtgggttg gcgttcatta
    28201 ttggcggcat tgtgatcctc gggagtacgg tgtaatgggc ctgatgtggg gattattcag
    28261 cgtcattatc gcctccgctg cgcaattaag cctgggtttt gcggcgagtc atctgccgcc
    28321 aatgacgcac ttatgggatt ttatcgcggc gctgttagct tttggcctgg atgcccggat
    28381 actgttactc gggctgctgg gttatctgct ttccgttttt tgttggtata aaacactaca
    28441 taaacttgcg ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat
    28501 tgcttcaatg gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt
    28561 agcctgtatt atgagcgggt tgatgctgat tttcctgccc acaacaaaac aacgttactg
    28621 agttttccct gccgctttac actgccgttc ccactcatcg gggctataac agcttgcaga
    28681 aagaactttc accgtgtgca gcttttcacg attaatacag taaagcgcat tctgcaaagg
    28741 cgagagtaaa tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat
    28801 cgcgccgcca gcatcgcaga gcataattac atgtctgtta tcttgtttat tgcagcacgt
    28861 ttttttgacc agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta
    28921 cagattaaca gataaactct ttatcaacgg caatggaatg gcagaaatga gaagtgaaac
    28981 ggagggttaa ttattgctgg cgttgcaccc actcttttag cgcctgacgt gaaattttaa
    29041 taccgccgtt tttcagctcc ggcggcagag tcagccagcg caccggttgt tgaaaacgag
    29101 ccagcttatc tttcacccat tcgtcaagat caacgctttg ctggtcatac tccaccaccg
    29161 ctaccggccg atgaccaaac tccttgtcgg caacgggaac gataaacacc tgcaaaaccg
    29221 caggatgtgc agcaataaca cgctcgactt cttccggctg aataccctca ccgccactga
    29281 agaacagatt atctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat
    29341 cgcgcgtggc gtaccagcct tcgtcattaa ccagtggaac ccgttgcccg ttacgccagt
    29401 aaccttctgc catactggca gcccgcagcc acacttcatc attaacgatt ttcacttccc
    29461 gacccggcag cggcgaacca acgtctgcca gaccgtcggc ttctttcgca cacactgtgg
    29521 aggcaaactc ggtcagacca tagccgcaaa agcaccgaat cccctgctcg cgcgcctgtt
    29581 ccgtcaactc gaccgggata gccgcgccgc caagtaaaac cgctttcagg gaaacggaac
    29641 tacggttaac cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg
    29701 ccagcatctg ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc
    29761 agcgccacat aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat
    29821 cgtgatcgcc aaacggaatc agcgataaca ccccttcggc gctgacaaga tgagcctgat
    29881 aagtatgaac agcggctttc ggcaagccag tggaaccaga ggttaacgtc attgagcaca
    29941 gacgcgtcgg ctgccacgcc gcggcatgtg cgccttcaac cagatgaatg tgcagcgacg
    30001 ctaacgccgg aaacgtgttt tccccttccg gcaccagcgc aaattgcagc gtaagattgg
    30061 gcagcaattc ctcaagcagc ggttgcggca gttgagggtt cacgggcaac acccgcgccc
    30121 cgcattgcag taacgccagc caggccagca gcgtttgcgg cgtattccac gcccgcaaca
    30181 tcacgccgct gccctcaacc acaccctgca tcgcaaagct ggaggctaat tcatcgacgc
    30241 gagcacaaag ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc
    30301 cccgcacttg ccgccagtga cgccacggcc agtcagagaa gatcataaca gccgctccag
    30361 tgcatcaacg tccactaacg gcagtgggct ccccggccag cgacgaacct gctgcgcctg
    30421 catcagatcc agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg
    30481 cgccagttgc gttagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc
    30541 cagcgcatgc gccgcctgca cttgctcgcg tactttatcc aggctgcccg tgagcgtggg
    30601 tttgataact accgcgcgca cgccctcttc agctacaaag gcaaaatccg gctcgcgcaa
    30661 gctttcatcc caggcaatgg caatgccggt ttcacgggca aacgcacgcg aatcatcgcg
    30721 ggttttgcac ggctcttcga gaaacgcgat gcggtgacga taatccgggt taacgtattt
    30781 ggcaaactgc tggcctttca gcggtgtcca ggcgcggttc gcgtcaagac gcaaatgcag
    30841 atcaggaatt gcctccagca acagattcac caccatgccg tcgcgcacgg cttcgtacaa
    30901 tcccactttg acctttgcca ctttctcgcc aggcatatcg gcaagtttga ggatcagatc
    30961 gtcaggatcg ccattacaca gcggtgccgc acggtaattg gccgcctgcg ggagcgtatc
    31021 tgccagctcc gccaatgcac aacttacgcc aaaggcaacg gaaggcatct gcggtaattc
    31081 gcaatcgcct gccagccagt tatttaccca ggcaagcagc acactttgcg catcttccca
    31141 gctttcctga ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccctc
    31201 acgcaggcaa acatacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc
    31261 cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta
    31321 aatttgctga aatcaggctg acgtttctgg ttgaaagcat tgcgaccttc ctgaccttct
    31381 tccgtcatgt agaacagcat ggtagcgttg cccgccagct cctgcagccc cgcctgcccg
    31441 tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc
    31501 atttcacggc accaacggac ggtctctttt tccagatccg ccagcggtac aacggtgttc
    31561 accaggccca tatccagcgc ctgttttgcg tcgtactgac ggcacaggaa ccagatttca
    31621 cgtgcttttt tctgcccaac aatgcgcgcc atataggaag cgccccagcc gccgtcgaag
    31681 gaaccgactt tcgggccagt ctgaccgaag atggcattat cagccgcgat agtcaggtcg
    31741 cacatcatgt gcaggacgtg accgccaccg atggagtagc cagccaccat cgcgacaacc
    31801 ggttttggac aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg
    31861 gaatcatctt tatagccgcc gtaatcacca cgtactttct ggtcaccacc ggagcagaac
    31921 gctttatcgc ctgcaccagt cagaatgatc acgccgatgt tgtcgtcata acgcgcatcc
    31981 gccagcgcct ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga
    32041 cgattaatgg tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcaaaa
    32101 ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc
    32161 atattgtgtc ctttagtcag aaacgcaaga tctgcgccag acttgcgatt acgccagcgg
    32221 gattttcccg gtgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt
    32281 ccgccgccag agcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg
    32341 tgcgggcgct aaggttagca cgtaaatcgg gctggacggc gagagaagtc gcctccagca
    32401 tggcggcaag ggttgcgcca ttattgttgc tgcgcagcgc caccagctcc cggcgttgat
    32461 cgtcattgag cgaggcaaaa acaggctgtt gataccaatc ggcaaatacc gccgttaacg
    32521 gttctgtacg aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcgcgttgtt
    32581 cagcattttg caacccagga tgcccgcctt cgacaacaac tccacaaagc cccgccagct
    32641 cctggcaagc cgccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt
    32701 taaggatgtt gtaactaacc agagttttac acaataagcc ggtgacatca tcaaatccat
    32761 cgacgctgat cgtcgctgaa ccaccgtgac ctgggagatc aacatacaac cgcgagtagt
    32821 cggcaaacgc ctcgcccact tcttgccatt catggcaatc gccagaaaaa ccgtgcaaaa
    32881 acaccaacca gggtaaacct ggtttgccgt gttttgcctg cgcgtgcagg atcataaatg
    32941 gcttacctgc gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat
    33001 ttcaatcacc gtggtggtcg gcgtacgcca ggcatcgact agtgtcgttt caagttcctg
    33061 ccagttttgc ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac
    33121 gttttgcggc atcagataga aacgctcgcg ctcgcttttc ggcgttggca acagcgaaaa
    33181 aatttgcccg ccgttgttgt tcaccacaat taataccagc ggtgcggaaa cctggcgcaa
    33241 taacgccagc gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg
    33301 tttgccgctt gcccgctgaa cgccggcggc agtcgagagc agcccgtcga taccgctggc
    33361 ccctcggttg ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg
    33421 caccaccagg ctgttaccga caaacaattg cccctgttca ggcaagtagt cgctgatgcg
    33481 atgcgccagt tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg
    33541 ttccgccagg cgcgggattt caaagcacca gggctggcgt ttttctgccg gatgcagctc
    33601 cagccagtcg gcaatattgg caattaagcg acgtccgcga tggtgtgccg gatcaagtcg
    33661 cccttcaatg tcatcaacaa tccagtactc ttctggttca cagcttgcct gccattggag
    33721 gagtcgtttc cccgtcaggc tgcttcccag ttgcaccact atttgtgcct gttgcagttc
    33781 gctggtcgct ttggcattgc ctaaccagag atcggcacac ggcagcggct gtccggtttg
    33841 cgacagcacg tcgccaatca gcggccagcc aagagtttgc gcccacagtg caactttttt
    33901 gccctcttcc gcactcatgc gcccggcaac caccacgccg cgcttttgtc gccagaagaa
    33961 ccagtcgcgc tgtttttcac tttcccgacg aggcgcttca cgcagccacg gtttgtcgtc
    34021 ctgccaccag tcgcccagcc gctgttgcca gctaatcccg gtgtcgtcca tttcgccata
    34081 cagcggttca gcaaacgggc agttgatatg gactccacca gcatgaagcg taccgagagc
    34141 gtggtcgatg gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcagtga
    34201 aatactgtgc gtggggtgag aggcgaacat tcccggctga cgaatcgcct gattcgcgcc
    34261 gcagtcaatc agctccggcg ggcgatcggc ggttaacagg atcagttttt ctccggttaa
    34321 cccagcttca atcagtgccg gatagagatt tgctaccgcc gtgccggagg tcacaatcac
    34381 cgccaccggc tgcttgctga ctttcgccag ccccagtgcc agatgcccca gtccacgctc
    34441 atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt
    34501 agaacgcgag ccaggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag
    34561 aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa
    34621 tattgcggct aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg
    34681 cgactcatta ctattccatt tgtaataaag tacgcagccc tgccgctttg ttgttgattt
    34741 cctgccactc ttgctcgggg tcagaaccac ggacaatgcc tgcgccagca tatagccgca
    34801 cgatattgcc gctgattttt gctgagcgca gggaaacgca gaattcactt tgctgtaatg
    34861 agagatagcc cgcagaaccg gcgtaccatt ctcgagtgaa cggttcgtga cgggcgataa
    34921 actgtcgcgc cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta
    34981 aacagatcac atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgta
    35041 ctttacgtag acgcagtacc tgcggcggta aaacatccag cgtttgggta tcggcctgca
    35101 atcgttggca gatatcttcc accaccaaca tgttctcgcg ctggttttta tcatccgcca
    35161 tcagccactc acctaactgc tgcgcctgct tatcatcagg atgatttgct accgttcccg
    35221 ccagcgcttc ggtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagaac
    35281 caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattgagcc
    35341 gacgactggc agccatcatc gctgccgcat tgaccggact tgcgaaatgc aggtcagttg
    35401 cccgagcaag cacaactttg tcgagctcgc cttcggcgat ggttttcgtt gccagttcga
    35461 ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta
    35521 atccaggcaa gggcttgata ctcaccagtg tggcgataaa ttcttttgcc ttgattgcat
    35581 cgtgctgaag ggagctttcg ctgaatagcg tcaaccgcag cgtggcttta ccgccacaac
    35641 gtcgccattc cagacgtggt aaaagtaaat tgccctgcga cgggtcaaac gcattcaacc
    35701 cccagatgcg taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca
    35761 acgacgtaaa acgggtaatc gcccccagga cggcagcttc ttcatcacca ttacgttgtt
    35821 gccagtagaa ttgcggatac acctgctgac tggccagcca gctcaaggca tcaaaagcgt
    35881 ctttgagagg gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg
    35941 acaaatggcg cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct
    36001 gttaaaagcc tcacattata cggggtacga caaaaaaaat gcagtacccc ggtgtaggga
    36061 ggttttaacg gcgtgccagc aacagtccca gaaccagccc aacggccgca cccacaccaa
    36121 ttccttgcca gggtttttcg tggacgtagt catcagcacg ataaacagcc tgcttcgccc
    36181 gatagtaata actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt
    36241 ctgcacgcgc tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca
    36301 cctcttccag tgtttcacta agcagcgtca ggtcatcatc aatacgtgta tcaccaaact
    36361 gattagacat tctcgttctc catttgtgta aaacctgtca ctaactatag acaagggtta
    36421 ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat
    36481 aaatatcggt cacagggatg aaaccaaatt gcccataaaa gttttgtaaa tgcgcctgcg
    36541 cccccagata aatcggcttc tccggccagc ggcgagtaca cgattccagc gccttactca
    36601 tcaattgctg accgattttt tcaccgcgta aggcttcgct gacaatcacc cgaccaatca
    36661 caacgggttg cagttcgtcg tcacttttca gaatcctcgc atacgccacc agtgtcccgt
    36721 tatgccaacc gagaatgtgg cgattgtctc cctccagatc gtcaccatcg atatcctggt
    36781 aaggacagtt ttgttccact acaaataccg cacaacgcag ttgtaataag gcataaagct
    36841 gagaaacgga taaatcagaa tgatgcagat cttgccagtc gatcatggaa ggctcctttg
    36901 tgctgtttgc tttatactag ccggaatatt ttcagcagcg cgatgaactc atggaattaa
    36961 tttttttagg tacttcagcc ggagtgccaa cccgtacgcg taatgtcacg gcaatattgc
    37021 ttaacctaca acatccgacc cagagcggac tttggttatt tgactgcggt gaaggcaccc
    37081 agcatcagct actgcatacc gcctttaacc ctggaaaact ggacaagatt tttatcagtc
    37141 accttcatgg cgatcatctc tttggtttac ccggcttact gtgcagtcgt tctatgtcag
    37201 gcattatcca acccttaacg atttatggtc cgcacggtat ccgtgaattt gtggagaccg
    37261 cgctgcggat tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg
    37321 gcgaaatttt cgatgatggc ctgcgcaaag tgaccgctta tccgatggaa cacccactgg
    37381 aatgttatgg ttatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat
    37441 taaaagctgc tggcgtgccg cctggcccgc tgtttcagga attaaaagcg ggcaaaacaa
    37501 tcatgctgga tgatggaagg cagattaatg gcgcagatta cttagccgct ccagtgccgg
    37561 gtaaagcgct cgctattttc ggcgatacgg gcccctgcga tgccgcgctg gagctggcta
    37621 aaggcgtcga cgtaatggtt cacgaagcga ctctggatat ggcgatggag gccaaagcca
    37681 acagtcgcgg ccatagttct acacgccagg cggcggcact agcccgtgag gctggagtcg
    37741 gtaagctaat cattacccac gtcagctcgc gctatgatga caaaggttgt cagcacctgt
    37801 tacgtgaatg tcggtcaatt ttcccggcga ctgaactggc gaatgatttc gcggtgttta
    37861 gcatttaagt cgaaagcggt ggttagttag cagattaatt taacgatacc aacgcattta
    37921 tttgacattt tttcatctgt ttgccggata cgtttgatgt cgatatcagg caaacagaat
    37981 tatcattttt agttttgtga attgtccaga tctttcgcca gtccaatcag gcgaataaat
    38041 tccgctctgt aaccctgtgg atcttcccct tttgccttct gagcccactg tttgatctgc
    38101 tgccaggagg tattgttcag gtattcagaa ccgcgtaact tttgcccata tgcagccact
    38161 gctgcgcgaa agcgcatatc ttctgaaggt gcctttattg cagatgccaa cgggaattca
    38221 actaactgac tttcttttcc ctgtggggat ttccaacgaa tctttaacca ggcaagttct
    38281 tttgttttgt ccgatttcgc tgatttgtta tccggggcat agcgtaactt atcaattgat
    38341 gctttttgcc ctttcagcgt taattcaaat aacaatgtta tatgtttacc tgcgccaata
    38401 tcacccgcat caacgttgtc gttattaaaa tcctctgcct taagttggcg cttttcataa
    38461 cctatctgac ggtattccgt tacccacgcg gggttaaact caatttgcgc tttgacatct
    38521 tttgctacgg taatcaacgt ctgccgcatt tcactgttca acactttctg cgcctctgag
    38581 agggtatcaa tgtagctata gttgccgtta ccaacatcgg caattcttac catcattgcc
    38641 tcgttataat tgctatcccc cacgccaagc gtcgacagag taacaccgga ctcacgctgt
    38701 tttttgacca ttgattcaat cgattttgga tcgtcaatgc caacgttaaa gtcaccgtca
    38761 gtggccaata aaatgcgatt gataccacct ttaataaacc ccttcgctgc ctgctgataa
    38821 gccatctcca gcccggcacc gccattggta ctgccttctg catccagcga atcaattgca
    38881 gcattaattt ccgccttgtg actaccggag atagaaggca acgcaatacg agagtcgcca
    38941 gcgtaggtca cgatggcaat gttatcctgc tcacgaagtt ctttgaccaa taatttcaac
    39001 gaagactgga taagtggcaa acgttcatca gaaatcattg aaccagaagt gtcgataaga
    39061 aagaccagat tagaagcggg taactcttca cttttgcgat ctttcgccag gatatcaact
    39121 ttcagcaatg ttcgctgttc attccagggt gcaggtgcca attcgtagcg catagcgaaa
    39181 ggtattggct tagaggccgg aatagattgt ttgtctttga tatcccagtc agacgggaaa
    39241 taattgacta tctcctccac ccgcacagcg tctggcggag gtaacagccc ttgattaagg
    39301 aaacgccgca cattcgcata actgccggta tcgacgtcaa ggctaaacgt cgccaacggg
    39361 ttttgcgcta cctgcttaac cggattatca tcgaactgct ggtagcgagc ggttcctgga
    39421 tttgcgattt gggaggctgc tttttctctg gctgcatgtt gatattttgg agccgcctgc
    39481 aatcgcccca gcaaagcctg tttgtctgaa tattgttgca cttcttgctg ggctaaggct
    39541 ttcgcgtctg cagcggcttt ggcggcgctt tgctcagcca cttttgttgc cgcttgttgc
    39601 gcggcaagca cttgctggtc agtgggtgta gtagattgtt gttgctgact tttcttatcc
    39661 tcaggttccg gcccacatcc tga
//
LOCUS       DAZKHK010000031        39474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKHK010000031 DAZKHK010000000
VERSION     DAZKHK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.031"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(100..300)
                     /locus_tag="ACHOS5_001645"
                     /pseudo
     CDS             complement(100..300)
                     /locus_tag="ACHOS5_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            404..2554
                     /gene="dinG"
                     /locus_tag="ACHOS5_001646"
     CDS             404..2554
                     /gene="dinG"
                     /locus_tag="ACHOS5_001646"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP9776468.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTISPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEFPEGIVKKKEKTKSPRRRRR"
     gene            2582..3544
                     /gene="ybiB"
                     /locus_tag="ACHOS5_001647"
     CDS             2582..3544
                     /gene="ybiB"
                     /locus_tag="ACHOS5_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP9776469.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDLAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3685..4770
                     /gene="hcxB"
                     /locus_tag="ACHOS5_001648"
     CDS             3685..4770
                     /gene="hcxB"
                     /locus_tag="ACHOS5_001648"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP9776470.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQVGMPEEALQAFCQQLAS"
     gene            4837..4896
                     /gene="ybiE"
                     /locus_tag="ACHOS5_001649"
     CDS             4837..4896
                     /gene="ybiE"
                     /locus_tag="ACHOS5_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP9776471.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(4910..5170)
                     /gene="ybiJ"
                     /locus_tag="ACHOS5_001650"
     CDS             complement(4910..5170)
                     /gene="ybiJ"
                     /locus_tag="ACHOS5_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP9776472.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5435..5701)
                     /gene="ybiI"
                     /locus_tag="ACHOS5_001651"
     CDS             complement(5435..5701)
                     /gene="ybiI"
                     /locus_tag="ACHOS5_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP9776473.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(5775..6452)
                     /gene="ybiX"
                     /locus_tag="ACHOS5_001652"
     CDS             complement(5775..6452)
                     /gene="ybiX"
                     /locus_tag="ACHOS5_001652"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP9776474.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6668..8950)
                     /gene="fiu"
                     /locus_tag="ACHOS5_001653"
     CDS             complement(6668..8950)
                     /gene="fiu"
                     /locus_tag="ACHOS5_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP9776475.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DLSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9215..9475)
                     /gene="mcbA"
                     /locus_tag="ACHOS5_001654"
     CDS             complement(9215..9475)
                     /gene="mcbA"
                     /locus_tag="ACHOS5_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP9776476.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            9751..10677
                     /gene="rlmF"
                     /locus_tag="ACHOS5_001655"
     CDS             9751..10677
                     /gene="rlmF"
                     /locus_tag="ACHOS5_001655"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP9776477.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10674..12899)
                     /gene="ybiO"
                     /locus_tag="ACHOS5_001656"
     CDS             complement(10674..12899)
                     /gene="ybiO"
                     /locus_tag="ACHOS5_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP9776478.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(13016..13738)
                     /gene="glnQ"
                     /locus_tag="ACHOS5_001657"
     CDS             complement(13016..13738)
                     /gene="glnQ"
                     /locus_tag="ACHOS5_001657"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP9776479.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13735..14394)
                     /gene="glnP"
                     /locus_tag="ACHOS5_001658"
     CDS             complement(13735..14394)
                     /gene="glnP"
                     /locus_tag="ACHOS5_001658"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP9776480.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14533..15279)
                     /gene="glnH"
                     /locus_tag="ACHOS5_001659"
     CDS             complement(14533..15279)
                     /gene="glnH"
                     /locus_tag="ACHOS5_001659"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP9776481.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15683..16186)
                     /gene="dps"
                     /locus_tag="ACHOS5_001660"
                     /gene_synonym="pexB"
     CDS             complement(15683..16186)
                     /gene="dps"
                     /locus_tag="ACHOS5_001660"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP9776482.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(16485..17372)
                     /gene="rhtA"
                     /locus_tag="ACHOS5_001661"
     CDS             complement(16485..17372)
                     /gene="rhtA"
                     /locus_tag="ACHOS5_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP9776483.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGTLQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            17607..17672
                     /locus_tag="ACHOS5_001662"
     CDS             17607..17672
                     /locus_tag="ACHOS5_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776484.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            17725..18240
                     /gene="ompX"
                     /locus_tag="ACHOS5_001663"
     CDS             17725..18240
                     /gene="ompX"
                     /locus_tag="ACHOS5_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP9776485.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18289..19872)
                     /gene="opgE"
                     /locus_tag="ACHOS5_001664"
     CDS             complement(18289..19872)
                     /gene="opgE"
                     /locus_tag="ACHOS5_001664"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9776486.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLMAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQI
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDNLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDNFDPKP"
     gene            complement(20144..20272)
                     /gene="mntS"
                     /locus_tag="ACHOS5_001665"
     CDS             complement(20144..20272)
                     /gene="mntS"
                     /locus_tag="ACHOS5_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP9776487.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20458..20925
                     /gene="mntR"
                     /locus_tag="ACHOS5_001666"
     CDS             20458..20925
                     /gene="mntR"
                     /locus_tag="ACHOS5_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP9776488.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20922..22040
                     /gene="ybiR"
                     /locus_tag="ACHOS5_001667"
     CDS             20922..22040
                     /gene="ybiR"
                     /locus_tag="ACHOS5_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP9776489.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSDPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(22098..23018)
                     /gene="ldtB"
                     /locus_tag="ACHOS5_001668"
     CDS             complement(22098..23018)
                     /gene="ldtB"
                     /locus_tag="ACHOS5_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9776490.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23237..24829
                     /gene="ybiT"
                     /locus_tag="ACHOS5_001669"
     CDS             23237..24829
                     /gene="ybiT"
                     /locus_tag="ACHOS5_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP9776491.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            complement(25029..25844)
                     /gene="ybiV"
                     /locus_tag="ACHOS5_001670"
     CDS             complement(25029..25844)
                     /gene="ybiV"
                     /locus_tag="ACHOS5_001670"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP9776492.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(25990..28422)
                     /gene="ybiW"
                     /locus_tag="ACHOS5_001671"
     CDS             complement(25990..28422)
                     /gene="ybiW"
                     /locus_tag="ACHOS5_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP9776493.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(28428..29327)
                     /gene="ybiY"
                     /locus_tag="ACHOS5_001672"
     CDS             complement(28428..29327)
                     /gene="ybiY"
                     /locus_tag="ACHOS5_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP9776494.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCKLCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEADIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            29458..30120
                     /gene="fsa"
                     /locus_tag="ACHOS5_001673"
     CDS             29458..30120
                     /gene="fsa"
                     /locus_tag="ACHOS5_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9776495.1"
                     /translation="MELYLDTSDVVAVTALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKSE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFKQDWLGAFGRT
                     SI"
     gene            complement(30196..30945)
                     /gene="moeB"
                     /locus_tag="ACHOS5_001674"
     CDS             complement(30196..30945)
                     /gene="moeB"
                     /locus_tag="ACHOS5_001674"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP9776496.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(30945..32180)
                     /gene="moeA"
                     /locus_tag="ACHOS5_001675"
     CDS             complement(30945..32180)
                     /gene="moeA"
                     /locus_tag="ACHOS5_001675"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP9776497.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNTDGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VEVGEWVEVEPFNALFGGL"
     gene            32384..33349
                     /gene="iaaA"
                     /locus_tag="ACHOS5_001676"
     CDS             32384..33349
                     /gene="iaaA"
                     /locus_tag="ACHOS5_001676"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9776498.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            33336..35207
                     /gene="gsiA"
                     /locus_tag="ACHOS5_001677"
     CDS             33336..35207
                     /gene="gsiA"
                     /locus_tag="ACHOS5_001677"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP9776499.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQHKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            35227..36765
                     /gene="gsiB"
                     /locus_tag="ACHOS5_001678"
     CDS             35227..36765
                     /gene="gsiB"
                     /locus_tag="ACHOS5_001678"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP9776500.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKEVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYHNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWSMPDTGFSFED
                     ADLQ"
     gene            36783..37703
                     /gene="gsiC"
                     /locus_tag="ACHOS5_001679"
     CDS             36783..37703
                     /gene="gsiC"
                     /locus_tag="ACHOS5_001679"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP9776501.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            37706..38617
                     /gene="gsiD"
                     /locus_tag="ACHOS5_001680"
     CDS             37706..38617
                     /gene="gsiD"
                     /locus_tag="ACHOS5_001680"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP9776502.1"
                     /translation="MRLFNWRRQTVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            38794..>39474
                     /locus_tag="ACHOS5_001681"
     CDS             38794..>39474
                     /locus_tag="ACHOS5_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFP9776503.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSW
                     NPAYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIA"
BASE COUNT         9431 a         9800 c        10606 g         9637 t
ORIGIN      
        1 tcgcgccgca tccggcattt cacaacaagc acttgtcagc catttgcgag cgcggcaaaa
       61 agatttctct gttttaacgc ccacaagcca cccgatgcgt tacttctccg cagccaattg
      121 tccgcgcaac gccattaaaa ggtaccccag gcgattcttg ccctgaccat taccgccatc
      181 tccccagtaa gcatcatttt gcgtatgctc aaccagtttt gcggacgcgg ttgccagcaa
      241 gagtgtacgc agttctgcat gttgttcgaa tttagcgcga agcgctttac gcatcatcct
      301 gctggttatt gcgagccgct ttccagaaac agaaaaacca ttacccctga aaaccgaaaa
      361 atgccacaat attggctgtt tatacagtat ttcaggtttt ctcatggcat taaccgccgc
      421 gcttaaagcg caaattgccg cctggtataa ggcgcttcag gaacagatcc ccgactttat
      481 tccccgtgcg ccgcagcggc agatgattgc ggacgtcgcc aaaacgctgg ccggagaaga
      541 agggcggcat ctggcgattg aagcccctac cggcgttggg aaaacgctct cctatttgat
      601 ccccggcatc gccattgccc gcgaagagca aaaaacgctg gtggtaagta ccgccaacgt
      661 ggcattgcag gatcagatct acagcaaaga tttaccgctg ctgaaaaaga tcattcccga
      721 tcttaaattc actgccgctt ttgggcgtgg gcgctacgtt tgcccgcgta atctgacagc
      781 actcgccagc actgagccca cgcaacagga tctgctggcg tttcttgacg acgaactgac
      841 gccgaacaat caggaagagc aaaaacgttg tgcgaagctg aagggcgatc tcgacactta
      901 taaatgggat ggtctgcgcg atcatactga tatcgctata gatgatgatc tctggcgtcg
      961 tttgagtacc gacaaggcca gttgccttaa ccgcaactgt tattactatc gcgaatgccc
     1021 gttttttgtc gctcgtcggg aaattcagga agcggaagtg gtggtggcaa accatgcgct
     1081 ggtgatggcg gcgatggaaa gcgaagcggt attgcctgac ccgaaaaatt tactgctggt
     1141 gctggacgaa ggtcatcacc tgccggatgt ggcgcgggat gcgctggaga tgagcgccga
     1201 aatcactgcg ccctggtacc ggctccaact ggacctgttc accaaactgg tcgccacctg
     1261 catggagcag tttcgcccga agaccatctc gccgctggcg atccctgaac gtttgaatgc
     1321 gcattgtgaa gagttgtatg agcttatcgc ctcgttaaac aacattctca atctctacat
     1381 gcctgccggg caggaggcag agcaccgttt tgcgatgggc gaactgccag atgaagtgct
     1441 ggagatctgc cagcggctgg caaaactcac cgagatgctg cgtggcctgg cggagttatt
     1501 tcttaacgat ttaagtgaga aaaccggcag ccatgacatt gtacgtctgc atcggttgat
     1561 tttgcagatg aaccgcgcat tggggatgtt cgaggcgcaa agcaaactct ggcggctggc
     1621 gtctctggcg caatcttccg gtgcgccggt gaccaaatgg gcgacgcggg aagagcgcga
     1681 agggcagcta cacctttggt ttcactgcgt gggaatacgt gtcagcgatc agctggaaag
     1741 gctgctgtgg cgcagtattc cgcacattat tgttacctcc gcaaccttgc gttcgctgaa
     1801 cagtttttcg cgtttgcagg agatgagtgg gctgaaagag aaagcgggcg accgttttgt
     1861 ggcactggat tcccctttta accactgtga acagggcaaa attgttattc cccggatgcg
     1921 cgttgagcct tccatcgaca acgaagagca gcacattgcc gaaatggcgg cctttttccg
     1981 tgaacaggtg gagagcaaaa aacatctcgg tatgttggtg ctgtttgcca gcgggcgtgc
     2041 gatgcagcgc tttctcgact atgtgacgga tttacgcctg atgttgctgg tgcagggcga
     2101 tcagccacgt tatcgcttag ttgaactgca ccgcaaacgc gttgccaacg gtgagcgtag
     2161 cgtgctggtg ggcttacagt cctttgccga aggtcttgat ctgaaaggcg atctgctcag
     2221 ccaggtgcat atccacaaga tcgcttttcc gcccatcgac agcccggtgg tgatcaccga
     2281 aggtgaatgg ctgaaaagcc tcaaccgcta tccgtttgag gtgcaaagcc tgccgagcgc
     2341 ttcgtttaac ctgattcagc aggttgggcg actgattcga agccacggtt gctgggggga
     2401 agtggttatc tacgacaaac gcttgctgac aaaaaattat ggcaagcgac tactggatgc
     2461 attaccggta tttccgatag agcaaccgga attccctgaa ggtatagtta aaaagaaaga
     2521 aaaaacgaaa tccccacgcc gtcggcggcg ttaatgatgt gagtcaggta aggagtcgta
     2581 aatggactat cgcaaaatca ttaaagagat tgggcgcggg aaaaaccacg cgcgcgattt
     2641 agaccgggat actgcgcgcg gtctgtatgc ccatatgctc aatggtgaag tccctgacct
     2701 tgagttgggc ggcgtattga ttgcgctacg tatcaaaggg gaaggggaag cagagatgct
     2761 cggcttttac gaagccatgc aaaatcacac catcaagctg acgccgcctg caggcaagcc
     2821 gatgccgatt gtcatcccca gttacaacgg cgcgcgtaaa caggccaacc tgacgccgtt
     2881 gttggcgatt ctcctgcaca aactcggttt tcccgtggtg gttcacgggg ttagcgaaga
     2941 tccaacccgc gtgctgacag aaaccatttt cgaactgatg ggtattacgc caacgttgca
     3001 cggcgggcag gcgcaggcga agctcgacga acatcaaccg gtgtttatgc ctgtcggcgc
     3061 gttttgcccg ccgctggaaa aacaactggc gatgcgctgg cggatgggcg tgcgtaacag
     3121 tgcacatacc ctggcgaaac tggcgacgcc atttgccgaa ggtgaggcac tgcgtctttc
     3181 cagcgtttcg catccggaat acattggacg cgtcgcgaag ttctttagtg atatcggcgg
     3241 gcgggcgcta ctaatgcatg gtactgaagg tgaagtgtat gctaatccgc agcgctgccc
     3301 gcagatcaat ctcattgacc gtgaagggat gcgggtgctg tatgaaaaac aggaccttgc
     3361 tggtagcgag ttactgccac aagcaaaaga cccagaaacc acggcgcagt ggattgagcg
     3421 ttgccttgct ggcagcgaac cgattcccga atcgctgaaa atccagatgg cttgctgcct
     3481 ggtggctacg ggtgaagcgg caactatcag cgacggcctg gcgcgcgtta atcaggcatt
     3541 ttaattcttt tctcagccgg atgagccatg tcatccggct tttaccccac cgcaatatga
     3601 aattcctgca tctttattga ccttcccgcg cccggcgtgc agcataaaaa aacaacaaac
     3661 acataacata aacaggagtt aaccatggaa agtggtcatc gctttgatgc tcagacgttg
     3721 cacagtttta ttcaggctgt atttcgtcag atgggtagcg aggaacaaga agcgaaatta
     3781 gttgccgatc atttaatcgc ggcaaacctg gcagggcatg attcacatgg tattggcatg
     3841 atcccaagct atgtgcgctc ctggagtcag gggcacctgc aaattaacca tcatgccaaa
     3901 atcgttaaag aggcgggggc ggcggtcacg ctcgatggcg atcgcgcatt tggtcaggtc
     3961 gcggcacacg aagcgatggc gctggggatt gagaaagcgc atcagcacgg cattgccgcc
     4021 gtggcgctac ataactcgca tcatatcggc cgtatcggtt actgggcgga gcagtgtgca
     4081 gcggcggggt ttgtctctat ccactttgtt agcgtggtcg gtattccaat ggtcgcgccg
     4141 ttccacggtc gcgacagccg ctttggcacc aatccgttct gtgtggtttt ccctcgtaaa
     4201 gataatttcc cgctgttgct tgattacgcc accagcgcca tcgcatttgg caaaacccgc
     4261 gtcgcctggc ataaaggcgt ccccgtgccg ccaggttgct tgattgacgt taacggcgtg
     4321 ccgacgacca atccggcggt aatgcaggag tcgccgttgg gttcgctgtt gacctttgcc
     4381 gaacataaag gctacgccct tgcagcgatg tgtgaaattc ttggcggggc gctttccggc
     4441 ggtaaaacga cgcatcagga aacgttacaa accagtcccg atgctattct taactgcatg
     4501 accactatca ttattaaccc ggaactgttc ggcgcgccgg attgtagcgc gcagaccgaa
     4561 gcctttgccg agtgggtgaa agcctcgccg catgatgacg ataagccgat tttgttaccg
     4621 ggcgagtggg aagtgaacac gcgtcgcgaa cggcaggagc aggggattcc attggatgcg
     4681 ggaagctggc aggccatttg tgatgcagcg cggcaggttg gtatgccgga agaggcgttg
     4741 caggctttct gtcagcagtt agccagctaa aaaaaagccc gtccagtggc ggacgggcaa
     4801 acaagggtaa cataggatca atgagggtta gagcatatgc gtctgtcggc aaacagacag
     4861 ggaaatactt gtgctggacg tagcgtaaac gcctgatccg gcttacgaat tacttataaa
     4921 ttaccgcagt accgcttaat ttattgttgt tggtggcaga agtgatgctg tatccactgg
     4981 caccggccgc cgcggctttc tcagccagtt tcgcttccag ggcatcgagg gtggatgcgc
     5041 catcggcaga aaccacgccg attttattca tgttctgtgc ctgggatgcc gttaccggtt
     5101 ccgccgcgaa cacgccaaat gacagagttg aaagagccat agcagcaaca acagtattga
     5161 tagttttcat aattaatctc tcgcaggtga tttttgtata agtgacgttg tttcgtcgat
     5221 gtgatgagta tcacgttttt ttacgagaga taaaatcgaa gagaattgac cgccttgttc
     5281 aaataaattg attgatatct aacatactga taaaccattt atttgttgta cttatggact
     5341 tgttttatac cgcgtggcag tcacggtagc gttaaaggca cggaatgcca cgcaaaatac
     5401 aaattatact cgctatacat cccgccagtt gtagttaacg taactggctg tctttcgaac
     5461 ctctgcgatt atatcctgta taagctggtt tttgtaaatc tttctcctgc tgacaatgaa
     5521 tgcataagcg cacgccagga atggcttccc gacgggcctg cgggataggg gcaccgcact
     5581 cttcacattc atacaggctt tcgccacgcg gaatttcacc ccgagcgcgg gcaatcgcat
     5641 cttcaattgt actgttgatc tgttcgttga cggcgtcatc attagcccaa ccggatgcca
     5701 tatcgtcctc cccatatcaa tacttgtaca gttaagtgta gctaatatag ggacgaactc
     5761 gggcagttca agcgtcagat ctccgaccat tcccgcagca gattatgata aagattgagc
     5821 agtgacagga tctcttcact ttcgccgtag cggcttttca gcgactgaat attgttgtcc
     5881 agttcaaaca gcatggcgcg ctttttatca tcacggatca tcgactggat ccacataaat
     5941 gatgccactc gcacgccgcg ggttacgggt gtcacgcaat gcaggctgct ggagggatac
     6001 aacaccagat cgcctgccgg gagttttacc cgatgttgcc cgaaggtgtc attaacgacc
     6061 agttcgccgc cgtcgtagct ttctggatcg cttaaaaaca gcgtggcaga aaggtcagtg
     6121 cgcatccagc cgttttgtgg atggctgcgt actgcgccat ccacatgaaa accataggtt
     6181 tcattgttct gatagcgatt aaacagcggc gtggaaaggg tacgcggcaa ggccgcagca
     6241 aagaacaaag catgttggtt aaccgcgttc agcacctcat tttgcagggc ggcgtacagg
     6301 gcgctgcggg tgtctacttg ttgattgttc ttaacttgcg caccttgtgc gccggtggtg
     6361 acgcgcccat ccacccattc ggcttgttcc agttgttcgc gaaaacgggc gacgtcctgt
     6421 ggcgataaca cgccgggaat gtggtacatc atacaagttc tccaaaaagt ggggcctgcg
     6481 ccccacaccg atttttaaca gcaggcaatg gtgctcaggt gccggatgcg gcgtgaacgc
     6541 cttatccggc ctaccaaatg atgtgaattc agtgtattgc acaaccttgt aggcctgata
     6601 agcgcagcgc atcaggcagt cctgctctcg ccatcaacat tagtgagcct tgcgccccac
     6661 atctgaatca gaaatgcata ttagctgtga gcaagaaggt tcttggctcg cccgggtgat
     6721 aacggtagcc gctcttattg attgaggcga cgtaatcggt atcaaacagg ttgtagacgt
     6781 ttagctggaa gtcgagattg cgattaaccc gataccccag tttggcgtcg gcgacccaat
     6841 aaccttcggt aaatgctggc gtccccaccg cgccgtctga acctttatgc atactgccga
     6901 tataccgtgc gcccgcacca acagaaatat catcggttgc ctgatactgg ctccataagg
     6961 tgaaggcgtg ttccggggta tacggcagcg atgaggaacc atcctgggca acatctttgc
     7021 cgtttttgat agttgctttt tgctgggtat agccgccaat catctgccac gcgggagtga
     7081 tattcccggc cacggatatc tcgtagcctt cgacacgttt cttaccatat tgcgaataag
     7141 tcccgtcatc attttgctca acttcatttt caatatcagt acggaacagc gcggcggtaa
     7201 gtagcaggcg tttatccaga acctgccatt tggtgccaat ctcgctggtg ttggcttttt
     7261 gtggtttaaa atcggtgcgg ttggcactgt taccgctgcc agactgcgca agggcgaagt
     7321 tgttgccgcc cggcggctgc tgggaaacgg catagttaat atagacattg ccgttttccg
     7381 tcaggtgata cagcgccccg gctttccagt tcaccagatt gcccgacttg gcggtgtcga
     7441 cggtggtgac cggagaacct tttgccacgc cagcagggca ggtgatggca ccgcgcccgc
     7501 tgccgccgca ggcggtagca ctgtcatatt cagtatgata attatccaga cggatcccgc
     7561 cgttcagctc aaaatcacgg gtgatttgca gcgtatcgaa ggcataaatt gcgaaggtat
     7621 ccgtctgacc attggcgttt gcgccgttgc gcgtcaggcc gccgggatga atgctgctgt
     7681 caggatgata aatatttacc gccggtaacg tcaccggatt aacgccatag ttagtctgtg
     7741 tttcgcgggt aaattccacg ccggtactga gatcatgacc gatagcgccg gtatagaacg
     7801 tcgaggtcag gttggtctgg ttggtgagaa ttttgttact cacatctttg gtattcgccg
     7861 tgcgtgacca cgtccagcta ttcacatcgc tggtgggctg ggtaatattc gacgctccac
     7921 ccataatcgc cgtcatcagg taatcctgct ttacgcgcga ccaacgcgta gtgttgcgaa
     7981 tggtggtgtt atcgttgatg tcgtgctcaa aacgcatggt agcggtgtcg gtggtcgaat
     8041 cgtcgtaatc ggaatctgtg ccgtaaaagt tatgagtatc aacttttccg gaatgattca
     8101 gggctgctgt tcccgcagat ggggcagaat aacccggtaa accgatagtc ggaatgccgc
     8161 cgtctggcgt gttgtgctgg gtgacatgca gataattaag atacaaacga ttcgctgtac
     8221 caaggccaaa agcgacagaa ggggcgacgc cgtaacgctc atttttgact ttgtcgcgtc
     8281 cggcatcgtg cgttttttcg cccattacat tcaggcgcac cgcagtggta tcaccaatga
     8341 cctgattgac gtccagcgtg ccgcggcgga accaggcgct gccaatactg gcggaggcgt
     8401 caatgccgga atcattgcgc ggctgtttgc tgatcatatt gatcgagcct gtcggcgcgc
     8461 tgcgcccgta gtcggtgccg gacggccctt taattacttc gacctgctcg gtattgaagg
     8521 tgtcgcgcga gacgctgccg atatcgcgaa tgccatcgat ataaatactg ttagaggtat
     8581 cggcaccgcg catataaatg gcgtcgccag tggtggagtt accgttctca cccgcaaaaa
     8641 acgcacccac gccggggacg tttttgagcg cgtcggtaag gttggttgca ccctgatctt
     8701 taatcacttg ttcagaaatc accgtcatcg tgcgggtagt atccgctacc ggacgcgaga
     8761 atttcggatc ggcagattgt tgtggcgcat aaagcgaagg cgttgatgct tcgacaacca
     8821 gcgtgtcatc cgcgttagct tgcccttcgg cagcgagtgc ctgtgccaca ggcgtaatac
     8881 caatacaaag accggcaaag aacgtgagcg aatgaaattg tctggcaggg aaattgcgat
     8941 tgttttccat tttgcaggtg actttttctt atatggtgaa attttcactg tcacgagaaa
     9001 gcatctctgc ggatgcgatg ccactgttaa tcccgtgata gcgtccgtgc aagtgataat
     9061 aattttgata agcattatca cttggcgcga ttttctatca cccattgcgc gaaaaagaaa
     9121 tgcatttatt tacattgcgt tcatatttga tgttttttgc ttacaaatcg gacgaaaact
     9181 gcatccagag gcgaatgcag ttacgttggt ggggttattt gtagatgatt gcagtaccga
     9241 acatctggtc atttccgcct gcggaattaa tgacataacc tttcgcgcct tgttcgcgcg
     9301 ctttttctgc cagtttatcc tccagatcgc ttaggtttga tgcgccagtt gccgaaacgg
     9361 taccggcggg ccgtagttga ccactgtcca ggttactcat cggttgcgcg gcataagccg
     9421 tgagagagat tccgctcagg acggtggcaa tcagtagtgt gaggcacttt ttcataatga
     9481 catcctcata aatacaacgg gtagtgccag ataagtgtag gtcgcctgcg cgatgaaaat
     9541 gacgcaaaaa attgacagag atgcgcttat tttttgaacg atatattttt acaaaataag
     9601 acaaatcaat gacatgcatg atgatgattt ttatggtgaa ttgccataaa ccatagccat
     9661 gtgaaggttt ttagaaatga taaacgcagc cagatattta ccctgtcgcg ttatgcgggt
     9721 atcttacgcc gctgcttaaa ggagaatgct atgtccgccc agaaaccggg gttgcatccg
     9781 cgcaaccgtc atcacagccg ctacgatctc gccacgcttt gtcaggtcaa tcctgaactc
     9841 aggcaattcc tcacgcttac acccgccggg gagcaaagcg tagactttgc caatccgctg
     9901 gcggtgaagg cgctcaataa ggcgttgctg gcccattttt acgccgtagc gaactgggat
     9961 atcccggacg gttttctctg cccaccagta ccgggccggg cggattatat tcatcacctt
    10021 gccgatttac tggccgaagc aagcggaaca attccagcta atgccagcat tctggatatc
    10081 ggcgttggtg cgaattgtat ttatccgctg attggcgtac atgaatatgg ctggcgtttt
    10141 accggtagtg aaaccagcag ccaggcgtta agcagtgcgc aggcgatcat cagtgctaat
    10201 ccggggctta accgcgctat tcgtctgcgt cggcaaaaag agagtggggc gatttttaac
    10261 ggcatcatcc ataaaaacga gcaatacgac gcgaccttgt gtaacccgcc attccacgat
    10321 tccgccgctg cggcacgggc aggtagtgaa cgtaaacgcc gtaacctggg gctgaacaaa
    10381 gacgatgcac tgaactttgg tggtcagcaa caggagttgt ggtgtgaagg cggtgaagtc
    10441 gcctttatta aaaagatgat tgaagagagc aaaggcttcg cgaagcaggt gatgtggttt
    10501 acctcgctgg tatctcgtgg tgaaaactta ccgccgttgt atcgtgctct gacggacgta
    10561 ggtgcggtga aggtggtaaa aaaagagatg gcccaggggc aaaagcagag ccgctttata
    10621 gcctggacct ttatgaacga cgagcagcgc cgccgttttg tcaatcgcca gcgttaaagc
    10681 gttggttccc ccggcggcaa cggttcagcc ggggtcgcgc ccggagcagg cagcacctga
    10741 taagtctgca ctggcgcgcg aacgcccgcc aggtcgaaat gttttttcac ctggctgtcg
    10801 agggcaaagc gtaccgtcca ctgtttgagt ggcagcgtgg tgaacgaaac acgcagtgta
    10861 aacgcggtat tgcttaagcc gacaatcccg gcaaagttcg gttcaccaat aaccagcccg
    10921 cgaatttctt cattttccat caattctgct accgcatctt tcagtgcctg attggcttta
    10981 tcagcatctt catggcgatc aacatcgtag tttgccacta ccgaaccaat accgcggacg
    11041 aagttagcaa aggtggttat cgaagaccac ggaatgatgt gatacgcccc ggtatcctgg
    11101 cgcacgccca cggagcgaat cgacatccgt tccacagtgc cggtcaacgg cccgatagtc
    11161 accaaatctc cagtgttcat gccgttttca aactgaataa atactccggt aataatatct
    11221 ttcaccagcg tttgcgaacc aaacgagata gccagaccta atgccccggc acctgccagc
    11281 aatggcgcga tattgacgcc gatttccgac aacacaatca tgatggtgat ggtactgata
    11341 atcaccgcca gcgcgttacg aaacagcgtc agcagggtac gcgtacgggc gctgggtagc
    11401 gggcggccat gaatatccga agccagccgg ttttctatca agctggcgag caccgtccag
    11461 ccaaccgccg agaagaaaag aatgagtgcg atacggatca ggatatctac ggttttctgc
    11521 cccgcaccgt tttgcagcca gttccagaaa tcgaacaatc cccatgcgct caacagcagc
    11581 attaccgcca cgcagactgt cagaatacgc gctgttttca gtgccgccga aagccatcca
    11641 ttcagccgtt tttgcagctc cggataattt cgctgagtat gtggcgagag ggtgatggtt
    11701 ttcgccagcc agcgggaaaa cataccggaa acaaatgctg cgataccaat aatcgccagg
    11761 ctgcgcaccg ttgcacccat cataaatttc aggctgttgc ccggatcgaa caacgaaaag
    11821 aaaaacagca cgataaaata ggcgcttgcc agccagtgcc acaccagcgc aaaggcgcgg
    11881 ataaacaggc tgaaaaaggc cagcgaatgc tccgcaaagt tgagcaaatg ctgggtgatc
    11941 tcttttttat tacgaaaaat caggtacaac gcccagacgg tcatgcacag cataatgatg
    12001 acgtttgcca gcgcacctat ctgtacattc acctgattag agataatcgg cacagccaca
    12061 atcagaccat agccaatcag gctgcttaac cagctcaggc gacgactcca gtaacgggcg
    12121 gtctcgtcat gaatcgtgaa cgggcgcagt tcagccacgt ttgggcaaaa aatcaggcgt
    12181 agtacggctt tgaaaaattc aatcagggca aaggcgttga gaaacaaact ttgttggaaa
    12241 gcgatggtgc gactgcccgc attcaggttg tcgcttaata cctggccgac aaacaatgtc
    12301 agcgccagta acagcaggtc gataataaac gccccgataa tcatcgccgg aagctgcaac
    12361 cagttactgc gctcacgatt tttttgccgc gcccactgac ccattttgcg atacagtggc
    12421 agcgcgcaca ggcgaatcag ccagtaaaaa ccaaacacta aaaccgctaa cattgaaaaa
    12481 tgggtcagcg cattgctgaa ggtttgtgga ttaaacggct tatgcgggga gccggtgata
    12541 ttgcgataaa gttgcccgaa gcgggcggaa agggcttcac cataatggcg gctgacttcg
    12601 gtgacctttt gcagcacggt ttgctcttca accagcgtcg gcggcacgat cttcggtacg
    12661 ggttccgcag gaggcgtcgc ggcgacggtg cgcaactggt cgatcaactc tttacgcgag
    12721 gtgtcattat ccagcacatc cgccagtgcg ccataggccg cttttttttg ttcgatatcc
    12781 ggttccgggg ccggttcagt cgttgagtcc gttgtcgttg tggttgtaac gccgggtatg
    12841 gataccgcgt gggcaggtgc gcccagcagg cagaagagga tgaacaggat ccaccgcatg
    12901 actcctccag tgagaaaaca gagcaaaaag ataagtatag atgctggggg agaggcgatt
    12961 ttaaatgaga ggaatctggt gtgcctccct ttcgggggaa agggaggctg agaagttaag
    13021 agacgtgctg caaaaattcc tgcaagcgct ggctcggtgg gttcttgatc aacacctgcg
    13081 gatcgccatc ttccgcaatc cggcctttgt cgataaagat caggcgcgaa gctactttct
    13141 cggcaaaacc gatttcgtgg gtcacgatta ccatcgtcat cccttcttca gccagatcct
    13201 gcataacctt cagcacttca tggcgcagtt ccgggtcaag agcggaagtc ggttcatcaa
    13261 acagcatcat tttcggcttc accgccagcg cgcgggcaat cgccacacgc tgctgttgac
    13321 caccagaaag ttcggaaggg tagtgatgtg cacgctctgc cagaccgact ttcgccagca
    13381 gctcacgcgc cagtttttcc gcctcttctt tattcgcgcc acgcacgcgt agcgggccaa
    13441 acatgacgtt ttccagcgcc gtcagatgcg ggaagaggta aaactgctgg aacaccatac
    13501 ccgcttcctg gcgaatcagg cgctcatcaa ctttcggatc gttaaccttc aggccatcga
    13561 caatcagatc gccggaggtg atttcttcca gtttgttgat gcagcgcagc agggtcgatt
    13621 taccggaacc ggacggcccg ataatcacca cgacttcgcc ctgagcaatg ttcaaatcga
    13681 tattgtgcag cacctgggtt ggaccaaaat gcttggagac gtttttaaat tcaatcacag
    13741 gattttcatc cttctttcca gacgacgcag aataaagctc agcaccaggg taataatcag
    13801 atagaacacc gccacggcgc tccagatctc aagggcgcgg aagttaccgg caataatttc
    13861 ttgcccctga cgggtcagtt ccgccacgcc gataacaata aacagcgaag tgtctttaat
    13921 gctgatgatc cactggttac ccagcggcgg cagcatacga cgcagtgcca gcggtaaaat
    13981 gacgtagcga atggtttccc aacgtgaaag accgagcgcc agtcctgctt cacgaaaacc
    14041 tttgtgaata gacagcaccg caccacgcgt gatttccgca atgtacgcgc cggagttgat
    14101 catgatggtg accaccgccg cagtaaacgg gtcgatgcgt aaatcgttaa acgccatcgg
    14161 cagggcgaaa tagataaaca tcacctggac gacgataggt gtgccgcgga tcacttcaat
    14221 aaagaccagc gcgacgtggt tggctatcca accaccgaag gtgcgtgcaa aacctgccaa
    14281 caatccgatc accagaccgc cagccagacc gaggaccgaa atccacaggg tcattttggc
    14341 accttcaatc agaagcggaa tggcaggcca gatggcactc cagtcaaact gcatatgttg
    14401 ttcctgttac cgtggtgtaa ataatcaaat tcagggtaat taatggcccc ggagcgggtt
    14461 tgcgcccttc accctaatcc tctccccggc ggggagaggg aactgccagt gcgctttaca
    14521 ggcgcagcgt tattatttcg gttcagtacc gaaccatttt ttgtagattt cgttgtaagt
    14581 tccgttctcg cgcagggttt tcaacgcgcc gttgactttg tcacgcagct cgtcgctacc
    14641 tttcgggaac gcaataccgt attgctgtgc ttccagagag tcacctaccg ctttgaactg
    14701 accgttaccg gcggttttga tgaagtacag aatgtttggc gtatcgtgca gaacggcgtc
    14761 tgcgcggttg gtgcccagtt ccatataggc gttatcgatg ttcgggaact gacgcagatc
    14821 tttagttttg atgtttgctt tcgcgtaatc aacggagcca gtgccgctct tcacagcaac
    14881 cactttcccg tcgagatctt tcacgctttt cacatcgtta ttgttagctt tcaccatcac
    14941 taacaggccg cttttgtagt agccgtcaga gaaatcgatc gcttttttac gctcgtcggt
    15001 gatggtaatg cccgccagcg ccagatcgac gtttttggtt tgcagtgctg gaatgatccc
    15061 actgaaatcc atcggcttca gttcgtaatc cagtttcagt tctttagcga tggcagccca
    15121 cagatcaacg tcaaagccca cgtatttatc gccctgttta aattcaaacg gaacgaaggc
    15181 agtatccgtc gcgacaacta attttttatc cgcggcatga gaagaaaccg caaaagccag
    15241 ggtcagtgca gccagtgaaa cttttaatac agacttcata gcatttcctt ttttctatcc
    15301 acgggacgat cccctgcgtg acattcacat atatgaaaaa atcgtgccag ttttgcaact
    15361 cattgatttt tcgagatgcg gcaaaaacgt gatgcacgat ttatagggca ataccctgaa
    15421 gatgcaccat tctggggcac caaactggtg cgctaaaatt gtgcactcaa cacagtgatt
    15481 atttagcgta aaacctgatg aaaaaacaat ctttatgtaa cgattgtgtg atgatgtgga
    15541 tacaaaaaat ttacttaacc agctggtgat agcagatgga tgcactaaat aagtgcgttg
    15601 aggcgggcta caaaacaaaa cggcctcctg tcaggaagcc gcttttatcg tgtactaaag
    15661 ttctgcacca tcagcgatgg atttattcga tgttagactc gataaaccac aggaatttat
    15721 ccaggtcgcg agacgcggcg gtcaggatat ctgcggtgtc gtcatctttc gcttcgccaa
    15781 tcgctttgcg tacgtcatta gcgacgattg cgtaacggtc agccagctct ttcaggtgat
    15841 cctgaacgtt gtggatgtcc agcgggtaac ttttcagcgg agttttgctg ttgataactt
    15901 gagtggtccc cagagctaca ccgcccagct gcactgcacg ttctgccatg gtatccagat
    15961 gatcgatcag tgcagtgcgg aagccatcca gcatttcatg tacggcaatg aagttagcgc
    16021 cgcgcatgtt ccagtgcgct tgtttggtaa tcaaagaaag atcaataaac tggataacct
    16081 ggcgattcag caactctact gttgcttttt tctcgctgtc agagacatcg ttgcgggtat
    16141 aaagcagatt ggtcgctttt gatttaacta atttagcggt actcataatt tcatatcctc
    16201 ttgatgttat gtcccagtaa ttaacgagac taagtatagc accagctatg tgttccgcta
    16261 ttctggctgt tcctatcaca ctaatagtgg taacaagcgt gaaaaacaaa accaataaaa
    16321 attcaataag ttagatatat tgataagaac aattcttatt tatttgctcg ccaggaattt
    16381 tcggaataaa tatgcttttg cataattcga tatataaaga cggtgtagag gaaaagtagc
    16441 gagaaaatct gcatggttat gcataaccat gcagaaatgt aaatttaatt tatgtctaat
    16501 tcttttattt tgctctcttt gcgtactgtc agcgtagaac ccattgaagc ggcgatgata
    16561 gcgccgagcg ccagcagctg tatgggtgtc agtgtttctc cgaggaaaat catcccggaa
    16621 acggcagcca gcgcaggttc catgctcatt agcgtaccaa atgtgcgtgt tggcaaacgg
    16681 gtgagggcaa tcatttccag cgaataaggc agagcggtcg agagaatagc gacagccaga
    16741 cccaatggaa taaccgacca gtgccagagt gcttcaccag cctgaagcgt tccaattggc
    16801 acgaaaatta acgctgcaat caacgaacca attgccaccg tcgcagggcc atgttccgct
    16861 ccagcacgtt gcccacttaa aatgtaaata gcccaacaag ccccggcccc cagtgccagc
    16921 gcacagccgg ttaaatcgac atgggaaacg tcttgcccca gcggtagcag gaaccacaga
    16981 ccaagaaccg ccagcacaac ccagacgaaa tctaccgggc gacgagaaga gaacagtgcc
    17041 accgccagtg gtccggtgaa ctccagcgcc accgcaatac ccagcggtac cgtctgaata
    17101 gaaagataaa aaagataatt cattccaccc agcgaaacgc cgtaaaacaa cagcggtaat
    17161 cgttgctctt tggcaaagcg cagtcgccat ggcttaaaga acgcgatgag aatcagcgtg
    17221 cctaatgcca gacgcagcgc agtgaccccc ggtgcgccca ccagaggaaa aagtgactta
    17281 gctaacgagg ctccaccctg aatagacgcc atggcaacga gcaatatgac tattggtaac
    17341 cagaccggca ttttacgtaa tgaaccaggc atcctttctc ccacaaatat ctaaacctaa
    17401 gtaaagcgtg gagtgtactg gatataccca atgctggttg agcatttgtt gaaaaaattt
    17461 tcccccgttt tgactaaaat gcgccaggat tgatggaatc attagtctgg tgattaggaa
    17521 taatctggat gaatgacagg gaaaacatgc gtaatactta cgcagttctc tgaaaaagtg
    17581 atttaaattt agatggatag cggtgtatgg aaacgttctg ttacatgaaa tggcccgtta
    17641 gacatcataa atcgcgaaga gtttcccatt aatttttgat atatttaaaa cttaggactt
    17701 atttgaatca catttgaggt ggttatgaaa aaaattgcat gtctttcagc actggccgca
    17761 gttctggctt tcaccgcagg tacttccgta gctgcgactt ctactgtaac tggcggttac
    17821 gcacagagcg acgctcaggg ccaaatgaac aaaatgggcg gtttcaacct gaaataccgc
    17881 tatgaagaag acaacagccc gctgggtgtg atcggttctt tcacttacac cgagaaaagc
    17941 cgtactgcaa gctctggtga ctacaacaaa aaccagtact acggcatcac tgctggtccg
    18001 gcttaccgca ttaacgactg ggcaagcatc tacggtgtag tgggtgtggg ttatggtaaa
    18061 ttccagacca ctgaataccc gacctacaaa cacgacacca gcgactacgg tttctcctac
    18121 ggtgctggtc tgcagttcaa cccgatggaa aacgttgctc tggacttctc ttacgagcag
    18181 agccgtattc gtagcgttga cgtaggcacc tggattgccg gtgttggtta ccgcttctaa
    18241 tcacttcggt gatataaaaa tccgcctctc ggggcggatt tttttgtttt aaggtttcgg
    18301 gtcgaaatta tcggttccga gatggttgta atccaccttc tgtaactgga agttggtgat
    18361 gtatatcggc ccggctttct tctcagatat aaacgggtat ttgatattta tttccttcgc
    18421 tttaattccc gtccactgcg agaaaaagcc taagaagtca ttggccgagc ggcgggcttt
    18481 aatcacccga tgcgctttat cgtcgctgga aatgaccata aaaggcacct ggaaattttg
    18541 ctgatactta tcatcatggg caaggtattg cacgtctttg ccgcgctctt taaaggccag
    18601 accgtggtcg gaaaagtaaa ccagcgagaa gctgctgccg ctgttgcgta actgatcgta
    18661 cagcttgcgc agtaaattgt ctgtttgcgt catggtatag agatagcacg acgtttcttt
    18721 tgattgcaca aaggtttcgt attttccttg tgtccggtcg caggcctgcg gatgcgagcc
    18781 catcagatgt agaacaatca gctgcggttg cgagtgctct tgcgccagca cttgagcggt
    18841 catatccagt aacgcttcgt ctttcgtgtt tttatctgct tcaaaattac cttctttcag
    18901 gaagtacact tcatctgctc gtttggcgat gctggcgatg gcggtatcgt attcgccgat
    18961 ttgaccctgg ttggaaaacc accaggtctg gaagcccgcg cgatttgcca gggtgacaaa
    19021 gttatcctga aactgtggtt tgccatcgac aacgcgattg agcgttaagc caagcgattt
    19081 ctgcgtggag ccactggcgg caatgtagtc agcaaatatc aaaccgttaa cgctgctggc
    19141 aaacggggta ttgtcccagt gaccgccaaa ggcaccgagg gcatcgcgac gcgcgctttc
    19201 tccgatcacc accacataga tctgatactt cggcttaacg gcagtgaccg tccaggtatc
    19261 tttcattccg gaaagttttg ccatccgttc ttgctcttca atcacctcgt tattattgac
    19321 gatgacatcc ttagcaaaac gaaataccgg atagccagta tctttcagtt taaatacgcc
    19381 gccccaggcc aggttttgca cgggagcaac aaaaaatgtc gctacactga ataccaggca
    19441 caggctgtcg aaggtattcc agcgcgcttt ttcactctct ttttttcgcc tgattgctat
    19501 tacgccgagc gcaaaaataa atatgccgac caggtagctg taccatggaa aaatcgtcag
    19561 gatttcggtc gactcttcca tattggtgga gtgcaatgcc agcaatgtat tgaagttagg
    19621 cgcgccgtag gcctgagcaa aagggaaata acaagccgcc atcagcgaac tgataccgac
    19681 cagaactttt tgtacgcgag gcaatgttcg ccatagcaaa agcaaaatag ccgtaaacgc
    19741 agcggtgtag agcaaactga aggggtaacc taagccgagg ttaattaata gcgactgtaa
    19801 aaagtagaac gcagtccacg ggctaaatac ccggctacgg gtaacaagcg attctttgag
    19861 ggttaaattc atatgccact atcgtaacaa acgccatgtg cttaccctgg cgtcaagggt
    19921 caatacctgc aagagtgcgt gaagagggga aaggggtccg catccgcgag ccgcaatata
    19981 cgcagggctg caagaagata gagcgagctg ctgttcaggt caactaccga cgaacaaatc
    20041 ttttgcgaag gggattgcaa atccgacaaa aagtggggtt tttcagacca aaacgcgttg
    20101 tgcctgcata aaagcaggag tgaagcggcg taccgcgacg ccgctattta tcggaaggtt
    20161 tatcctgctg cggtttgttg ttgaccatat cgcacaacat agagagcagc attaaccgta
    20221 ctttaaaggg agaatgacta aacacgcgca tacacctctt gaactcattc ataagacctc
    20281 ctgacttgct aatcccatcg atccttgagg gatgattgca ttacatacag atatagcaca
    20341 ggctatatta tatagctatt gctaaaacgt taattttttg taccttcgca actctggttt
    20401 acaatgtgcg cacgaaatga gaacgctatg tatgcgtcac cataatgagg aagcacaatg
    20461 agtcgtcgcg caggtacgcc aacagcaaaa aaagtgacgc agttagtgaa cgtggaagag
    20521 cacgttgaag ggttccgcca ggtcagagag gcgcatcggc gcgagcttat tgatgattac
    20581 gttgagctga tttctgactt gatcagggaa gtgggggaag ctcgtcaggt agacatggct
    20641 gctcgtctgg gggtttcgca accgacggtg gctaaaatgc ttaagcgact ggcaaccatg
    20701 gggctgattg aaatgatccc ctggcgtggc gtgtttttaa cggcagaagg agagaagctg
    20761 gcgcaggaaa gccgcgagcg acatcagata gtcgaaaatt tcttgttggt gttgggcgtc
    20821 agtccggaaa tcgcccgtcg cgacgcggaa ggtatggagc accatgttag tgaagagacg
    20881 ctggatgcct ttcgtttgtt tacccagaaa cacggtgcca aatgagcctg ccttttttac
    20941 gcacgctgca aggcgatcgt ttttttcagt tattaattct tgttggcatc ggattaagtt
    21001 ttttcgttcc ctttgcaccg aaatcctggc ctgctgctat cgactggcac accatcatca
    21061 ccttaagcgg cctgatgctg ctgaccaaag gtgtcgagtt aagcggttat tttgatgtgc
    21121 tggggcgcaa aatggtgcgc cgttttgcta cggagcgtcg gctggcgatg tttatggtac
    21181 tggcggcagc gctgctttct acctttctga ccaacgatgt cgcgctgttt atagttgttc
    21241 cgctgactat cacgctaaaa agactgtgtg agatcccggt taatcggctg attatttttg
    21301 aggcgctggc agtcaacgct ggttcgctac tgacgccaat tggcaacccg caaaatattc
    21361 ttatctgggg acgttctggt ctttcgtttg ccggatttat tgcccaaatg gcaccgctgg
    21421 ctggcgcaat gatgctgaca ctcctgctgt tgtgctggtg ttgtttccct ggaaaggcgc
    21481 tgcaatacca tacgggggtg caaacgccgg agtggaaacc gcggctggtg tggagttgtc
    21541 tggggctgta tatcgtcttt ctgacggcgc tggagttaaa acaagagctg tggggactgg
    21601 tgattgtggc ggcgggcttt gcgctgctgg cgcgtcgcgt ggtgctcagt gtggactgga
    21661 cgctgctgct ggtgtttatg gcgatgttta tcgacgtcca tttactaacc caactgccag
    21721 ctttgcaggg ggtgttaagt aacgtgagtc atttatccga ccccggatta tggttaacgg
    21781 cgattggttt atcgcaggtg atcagtaacg tgccgagtac cattttgctg ctcaattatg
    21841 tgccaccgtc tttattactg gcatgggcgg taaacgtggg gggctttggt ttattacccg
    21901 gatcgctggc aaatttgatt gcgttacgga tggcgaacga tcgccgtatc tggtggcgtt
    21961 ttcatcttta ttcgataccg atgctgttgt gggcggcgct ggtgggatat gttttgttgg
    22021 ttatgatccc ggcgggataa ggtctgtcaa attaaaaagg cggattatga gtccgccttt
    22081 gctttacgtt ttgaagatta attcagacga accggcatcc cggaacgatt tttgatcgct
    22141 tcatccagaa caacctggtc aacatctggc tgaccggtca ctgtctgcac gctcttcgtc
    22201 agggtaattg gcacaatttc ctgaccttca aactgggctt cagtggtaga cagcgggtta
    22261 tggacttcaa tataacggct gccgtctggc tcggtggtcg cttttaccgg ctcatcaata
    22321 aactgtacgc gggtaccgac cggtactttc tcgaacagga atttgatgtc ttcgttacgc
    22381 agacgcacac aaccatgact tacacgcagg ccgataccga agttggcgtt ggtgccatgg
    22441 atagcgtaca ggcgaccgat gtagagtgca tacagcccca tcgggttatc cggaccagcc
    22501 ggaacgaccg ccggaagcgg ttcgcccgca gcgcggtact ctgcgtgcat tttagcggtc
    22561 ggcgtccagg tcgggcctgc ttttttacgc tcaactttgg tggtccagtt gataggcgtg
    22621 tctttgccta actgaccaat gccgatcggc agcacgataa cggtgttggt ccctttcgga
    22681 tagtaataca gacgcatctc tgcactgtta atgacgatgc cttcatgaac ggtatccggc
    22741 aggatcagct gctgcggaat gttcagtaca gtaccgcctt tcggcaggaa ggtatccaca
    22801 cccgggttcg cttccatcat attggaaagt cccatctggt actcggcggc aaaatactcc
    22861 agcggctgag tgttaccttc aggaatggtg atcacctgat tctgaccaac caggcgactc
    22921 ccgtcggttg gcagaggata agttaccgca gaggcggtac tgcaaaagcc gacaacagcg
    22981 aaggccgctg cgaataatgt tttcaatttc atattcatgt taagcgagat tttgtgcctg
    23041 gcaggccatt gggttgagaa tattagagta ctggaagcgc attataagtt cattccagct
    23101 cacagtgaaa tcagatgtgt acgaaatcac attttttgcc tttggcttga gtgtagacct
    23161 taagcgagga gcaggatctt ctttcagact tatggcataa tgcgcggttt gtcatatctc
    23221 ttttcaggat acgcctgtgt tagtttccag taacgtcacc atgcagttcg gcagtaagcc
    23281 gttgtttgaa aacatttccg tcaaattcgg cggcggcaac cgttacggcc tgattggcgc
    23341 gaacggtagt ggtaaatcca cctttatgaa gatcctcggc ggcgacttag agccgacgtt
    23401 gggtaacgtt tccctcgatc ccaacgagcg cattggtaag ctgcgtcagg atcagtttgc
    23461 ctttgaagag ttcactgtgc tggatacggt gatcatgggg cataaagagt tgtgggaagt
    23521 gaagcaggag cgtgaccgca tctatgcttt gccggaaatg agtgaagaag acggctataa
    23581 agtggccgat ctggaagtga aatacggcga aatggacggt tactctgcgg aagctcgcgc
    23641 cggtgaactg ttgcttggcg tgggaattcc agtggaacag cactacggcc cgatgagtga
    23701 agttgctcct ggctggaagc tgcgtgtgct gctggcgcag gcgctgtttg ctgatccgga
    23761 cattctcctg ctcgacgaac cgaccaacaa cctcgacatc gacaccattc gctggctgga
    23821 acaggtgctg aacgagcgtg acagcaccat gatcatcatc tcgcacgacc gtcacttcct
    23881 taacatggtt tgtacccaca tggcggatct ggattacggc gagctgcgcg tttatccggg
    23941 caactacgat gagtacatga cggcggcgac ccaggcgcgt gaacgtctgc tggctgataa
    24001 cgccaagaag aaggcgcaga ttgctgagct gcaatctttc gttagccgct ttagcgccaa
    24061 cgcctcgaaa tctcgccagg caacttcgcg cgcgcgccag attgataaaa tcaaactgga
    24121 agaggtgaaa gcctccagcc gtcagaaccc gttcatccgt tttgaacagg ataagaaact
    24181 gttccgtaac gcgctggaag tggaaggtct gaccaaaggg tttgataacg gtccgctgtt
    24241 taaaaatctc aacctgctgc tggaggtcgg tgaaaaactg gcggtactgg gtaccaacgg
    24301 cgtcggtaaa tcaacgctgt tgaaaacgct ggtgggcgat ctgcaaccgg acagcggcac
    24361 cgtaaaatgg tctgaaaacg cgcgcattgg ttactatgct caggaccatg aatatgagtt
    24421 cgaaaatgat ctgaccgtgt tcgaatggat gagccagtgg aagcaggaag gcgatgacga
    24481 gcaggcggta cgcagcattc tcggtcgttt gctgttcagc caggacgaca tcaaaaagcc
    24541 agctaaagtg ctttccggtg gggaaaaagg gcggatgctg tttggtaagt taatgatgca
    24601 gaagccgaac attctgatca tggacgaacc gaccaaccac ctggatatgg aatctattga
    24661 gtcgctgaac atggcgctgg aactgtatca gggcacgctg atctttgttt cacacgaccg
    24721 tgagttcgta agctccctgg cgacccgtat tctggaaatc accccggaac gcgtgatcga
    24781 ctttagcggt aattacgaag attacctgcg tagtaaaggg atcgagtaag ttattatcag
    24841 tagccctgat atggtcaggg ctactgaaac tgatgacaaa cgcaagattg cctggtgcgc
    24901 tacgcttatc aggcctggta gtgtatcgca atatattgaa ttttcatgat tttgtatgcc
    24961 aaataaggcg gtcacgccgt atccggtata atgacatgca ctttctcaat aaactgaatt
    25021 ggcgataatc aaagattaaa cggcggttta ttttccagca ccgcctgaat cacattcagc
    25081 gcgccttcat gattattatc atcggtagcg taacgggcga tttgtttaat gttttccgca
    25141 gcattaccca tcgcaaagga ataacgcgcc attttcagca tctccgcatc gttaccgctg
    25201 tcgccaatcg ctaccacatt ttgcggtgac agatcccagc gtttcagtaa ccgcgaaata
    25261 ccgtttgctt tatgtagacc gggaataatc aagtcgataa agccaaaacc actggtgacg
    25321 ggtttcataa tgccatccag cgctatgtgc agtttgtcga tcactaacgg gatttgttca
    25381 tccggcaggt tgagcgaaaa cttgaacagt acgtcgtcaa tctcctgata atcttttaca
    25441 ggtttcaggc gatggtagtg ttttgccatc agtgcgacga atgcttcggg ggcattttcg
    25501 ctgacatatg cactttgcag accgcaggcg acaaaattga gttgcttatc ttttagcaac
    25561 tcgccaataa caatccgcga ttcatgtcgg gtcagttcgc cgtggaacaa ctgcttgcca
    25621 tgttcgtaaa ccagtgcgcc gttttccgcg acaaaagaga tctcatcctt tagctcagga
    25681 aagaacgaaa taagctggta atactgatta ccgctggcga caacgaactc aatgccgcgc
    25741 tttttcagtt cctgatattg cgccataaaa cgtggttggt tgtacgtttt ggcgtcgtta
    25801 agaaaagtac cgtccatgtc tgtgacgata acttttacgc tcataggtgt gctcctggct
    25861 cgaaaatgaa accgtaacag tgtaataaca atgtgacgca gagcacaaat tatatttcga
    25921 atgaaagcga ggctgaaatt gatgatgtga atgatttggc ccggcgacga cgacgccggg
    25981 ctgaggggat tacagcatgt gttcggtacg ggcgatgata tcgtcctgtg cgtctggaga
    26041 gagcgcggtg aagaaggcgg aataacccgc tacgcgtacc accagatcgc ggtattgatc
    26101 cggatgtttc ttcgcctcca gcaatgtttc gcgggaaaca atgttgtact gaatatgcca
    26161 gcctttatgc acttcaaaga aggtacgcag caggatcatc agtttctgct tgtcagattc
    26221 attctccagc gttgccggat tcagtttctg gttgagcagc acgccgccga gaattgctgc
    26281 cgtaggcagt ttacccaccg agccaatgac cgcagtaggg ccgagatggt cagtaccgga
    26341 ggccggacta gcgccttctg ccagcggggt atgtgcttta cgtccgtccg gcgttgccat
    26401 agtctgcgcg ccaaacggta cgttagcgga gatagacgat gtgcccgcat aatagttgcc
    26461 gccaatcgga ccacgaccgt agcgcgggtt atggtactgc ttcagttcgt cgatataggt
    26521 ctgataagcg cgagccagca gcgtatcgac agtatcatcg tcgttgccgt acttcggcgc
    26581 accgttaatc agccgctgac gcagttgctc gtgagtcagg ccgtcgaagt catccgccag
    26641 tgcggcagcc agctgttgct gaccaatcgc gccctgttca aataccagtt tcttcactgc
    26701 cgccaggctg ttgccgaggt tagcaatgcc gacctgcagg ccagaaaccc agtcatattt
    26761 cgcgccgcct tgcttgatac ttttcgctcg ctcaatacag tcatccacca gcgccgagca
    26821 gagaatatcg tgtacgttct cttccagcat ggtatcgacg acatattcga tttcgattga
    26881 tttacgcgtg tagtaacgga tttgcgtatc ccatgcgtcc attacttcat cgaagttgtt
    26941 gaagttaccc gctgacaacg ctttttcttg tggcaggaac actttgccgc tggtggcatc
    27001 acgaccgcct tccagcgccg ccagcatcac gcgggcgaag ttgataaagc tcatgccggt
    27061 acagcgatag ccccatttgc caccgacggc ggtttcgata caaccaatcg ctgcatagtc
    27121 gtaagcgtcc tgcggttcaa taccgagttt aataaattcc gggatgacga tttcgtcgtt
    27181 gttgaacgcc ggcatcccga agccgcagcg gatcacctga acacaggcgt cgaggaaatc
    27241 gttactcata cccgcatggt aacgcacgct gaggttaggc tgagtcgaac gcaggcgacc
    27301 gcaagattcg aggatcgcat aagagagtgg atttaccgcg tccatcggtt gaccatcaac
    27361 cagattttgc ccgccaatgg tgacgttctg atacagcgga cttcccgcag aggcttttga
    27421 gtgtgagccg gagcggattt tgttcacttc cagcaatttc aaccagcagc tatgcagcat
    27481 ctcgatggcg tgttcgcgat ccagtgtctg gttcagttca acgtcacggc gatagtatgg
    27541 atagagatac tggtccatac gaccaaacga taccgagtga ccgttagatt cgatctgcaa
    27601 aatcaactgg atgaagtaac atagttgcag cgcctgccag aaagtctgcg gcggctggtg
    27661 ggcgataaga tcgcagtttt ctgctatcgt cagcagttca tcgcgacggc tttcgcgggt
    27721 ttcggtcgcg gccatttcac gcgccagggc agcgaaacgt tcaatgtgtt cactgactgc
    27781 caccagcacg atatcaatcg ctttcaggaa ttgctcgccg tgcagatctt ccagaaccgt
    27841 cagattgatg cgcgagcgac gttccgccac tttctcacgc agaccatcaa gacctttttc
    27901 cagcagtagc gggaaattga ccgccaggtg cgcatcgccg gaagtcatat tgccctccgc
    27961 tttaatgatt ccggtcgcca gcagaccttt ttgctcatcg gtaaacatgc cgtagcagcg
    28021 atcctgtacg gtctgaccgc gccaccacgg gcacacttca tgcagaacgc gtttgttctc
    28081 ttcgctcacc gcaaaaccag caccgggacg atctgccaga tcatcaatct ctttttcgat
    28141 ccacgagacg gtatattccg ggaagatcgg cgcggcgcga acttcgcttg cctggttgcc
    28201 aatgatcaat tcatcatgtt tgatccagat ggtgcgattc gccaggtgat gcgccagtgc
    28261 cagcgcgcga cgtaccggga ttggcttatc gagatgttgt tgatacatct cggtatagtg
    28321 ttgtgctcgt tcggtacaga ccggcggttt cacaatatgc accagcgcat ttttgtgcgc
    28381 tttaatgcgg tcgctgagcg tatccagttt tagtgttgtc atgattgtta tcctcgtaag
    28441 gtcgcggtta accctttctg gcaggcatac tgctgggcaa agtcgagcag ttctggcgca
    28501 tcaagcggtt tttccggggc gtcatagggc agattaagta agtgatattt gttgatgccc
    28561 agtgtgtggt agggcagaaa atgaatttcg ctaacgtgca gctcgtcggc ggcaaaatcg
    28621 gtaatggctt tcacagaagt ttcgtcggca ttaaagccct gaatcagtgg cacgcggata
    28681 atgatttttt tgccggctgc ggcgagtttt ttcaggttat ccagcaccct ggcggcgtta
    28741 ccgtcggtcc actgtttaaa cggcgcgtcg gcaacgtgtt ttaaatcggc aagaaacaga
    28801 tcgatatagg gcagagaagg ggcgatatat ttccacggca catgcagaca ggtttctacc
    28861 gcagtatgaa tgtcggcctc gtggctggct tgcagcagcg ccgtcgccat ttccggctgc
    28921 ataaacggtt caccgccaga aagcgttaag ccgccgccgc tgcgatcgta aaacggttta
    28981 tcgcgcaaaa cggttgccat gatctcctca acgcttttca cttcaccaca cacggttaac
    29041 gcctgtgtcg ggcagcagtc ggttagcgcc gtaaaatgct ccggggttaa cttttcccga
    29101 tgaataagca aaccattcaa cgcgcgctca atcacttccg gtgcggtctt agcgcacagc
    29161 ttacagcctt ccagacagag tcgtgggtca tacagcagat cctgcgtgcg ggcgcggctt
    29221 tccgggttct gacaccagcg gcagcccagc gaacagcctt taagaaatac gaccgtgcgg
    29281 ataccggggc catcatgggt cgagtagcgt tgaatattga aaatcatagt tgcctctctg
    29341 tttcgttcaa gcattaaaat actttcgaat gaaagttaga ttgatgtgcg tcaactgttc
    29401 agagagtttt cccgtgatag tctacattca gacaaaaagt acattttgag gatggttatg
    29461 gaactgtatc tggatacttc agacgttgtt gcggtgacgg cgctgtcacg tatttttccg
    29521 ctggcgggtg tgaccactaa cccaagcatt atcgcagcgg gtaaaaaacc gctggaggtt
    29581 gtgcttccgc aacttcacga ggcgatgggc ggtcaggggc gtctatttgc ccaggtaatg
    29641 gctaccactg ctgaagggat ggtgaatgac gcacgtaaat tgcgttctat tattgcggat
    29701 atcgtggtga aagttccggt gaccgctgag gggctggcag ctattaagat gttaaaatcg
    29761 gaagggattc cgacgctggg aaccgcggta tatggcgcag cacaagggct actgtcggcg
    29821 ctggcaggtg cggaatatgt tgcgccttac gttaatcgta ttgatgctca gggcggtagc
    29881 ggtattcaga ctgtgaccga cttacaccag ttattgaaaa tgcatgcgcc gcaggcgaaa
    29941 gtgttagccg cgagtttcaa aaccccgcgt caggcgctgg actgcttact ggcagggtgt
    30001 gaatcaatta ctctgccact ggatgtggca caacagatga ttagctatcc ggcagttgat
    30061 gccgctgtgg cgaagtttaa acaggactgg ctgggagcgt ttggcagaac gtcgatttaa
    30121 ccggacgttc tgcatcctca taaattgctg atgacgtggc ggagtgccgc gtcttatctg
    30181 gcctggaggt ggcagttact gcccgcacac ctcacacccc ggattacgca tcagtttcat
    30241 ttcgcgaaac tgacaggtca tcgcatcgta catcacgatt tttccgctgg ccggtttgcc
    30301 ataacctgcc agcagtttga tcgcttccat tgcctgcaac gaaccaatca ccccgatcaa
    30361 cggtgccatc acgcctgctt ctacgcaggt taacgcgttc tcgccaaaca aacggctaag
    30421 gcagcgatag cacggttcgc cgtcctgata agtgaagacc gtgatttgcc cttccatacg
    30481 aattgccgcg ccggaaacca gcggcacctt cgcggcaaaa cagcctgcgt tcagttgatt
    30541 acgtaccgca acgttatccg tacagtcgag caccagatcg tgttttgcaa tcatcgcagc
    30601 aagttcagcg tcatcaagca gcgcattgac tggcgtaatc gcgatatgtg ggttgatccg
    30661 cgtcagggcg tcacgggcgg attccacctt tggttgcccg accgtggcat cactgtgcag
    30721 cgtctggcgt tgcagattgg agagcgaaac cgtgtcgaag tcgagcagcg tcaggttgcc
    30781 gacaccggcg cttgccagat attgcgatgc cgcacagccg aggccaccaa gccccaccac
    30841 caacacgcga gaatccttca gcgcctcctg accgtcaaaa tcaaaaccgc gcagaatgat
    30901 ttgccggttg tagcgcagca tctcctgatc gctgagttcc gccattacag gcctccgaac
    30961 aacgcgttaa acggttctac ttccacccat tcgcccactt ccacattgcc gcgttcgcgt
    31021 tccagcacga taaagcagtt gccgaggcta aaggagctaa atatatgtga accctgatgt
    31081 ccggtggtcg tcacttccag ttcgccatcg gtgttgcgtt gcagcacgcc gcgctggaaa
    31141 tcaaggcgtc ctggcgtttt tttcaggcgg gacgctgtgc gtacacgctg gcgcgcgggc
    31201 aggccactgg cggtattgcc gcttagtttt gccagcaaag gctgtaccag ttgatagaag
    31261 gtcagcgtcg ctgaaaccgg gttgcccggc aggccgcaga accaactatt agaaagttta
    31321 ccgaatgcga acggtttacc cggtttaatc gccagtttcc agaaggcaat ctcccccagc
    31381 tcttcaagaa tcgttttggt gtaatccgct tcacccactg aaacaccgcc ggaactgatc
    31441 accacatccg cctggctgtc ggcttcaata aatgcggcgc gcagggcatg gggatcgtcg
    31501 cggataatcc ctaagttgat tacctcgcat cccaactgtt ctaacatcag gtgtacggcg
    31561 agacggttgg tatcgtagat ttggccgtcg cccagcggct gaccgggcaa ctggagttca
    31621 tcaccggtag aaaaaagcgc tacacgcact ttacgaatca ccggaacttc ggcaatccct
    31681 agcgaagcaa tcactggcag ttcagcggta gtcaggcgag tccccgccgg gaaaacaacc
    31741 gcacctgcag agatatcttc accgcgacgg cgaatatttt gcccgctacg cgcttcagca
    31801 gtaaaacgta cgccattgtc cgtttgttca gtctgctcct gcatcaccac cgcttcgcag
    31861 ccttccggca ccggcgcacc ggtcataata cgaatgcagg tacccgcagg ccattcacca
    31921 tggtacggct gaccggcaaa ggatttaccg gcaacgggca gcggttgccc ggaggcaata
    31981 tcggctaaac gcaccgcgta gccgtccata gcggagttat caaaccccgg gacatccagt
    32041 ggcgaaacga catcgctcgc cagaatacga ccaaaacact gaaccagtgg cagcgtttcc
    32101 tgggcggtca gtggggtaac gcgagaaagc atctcattaa gcgcggtgtc gagcgacatc
    32161 aatccggtgg taaattccat gaaaacactc ctgcggaggc aaaatcgaat ttgcctatta
    32221 tgtcagaaaa acgccacaga ctgtatgcca cctcgggcgt agcgctggct cctgccttta
    32281 catgccatat ccatctttct atattcaaaa attgaatgag tcattcataa aaattctgat
    32341 atttatagca aaagtggcga gccaccctta atggacgaat actatgggca aagcagtcat
    32401 tgcaattcat ggtggcgcag gtgcaattag ccgcgcgcag atgagtctgc aacaggaatt
    32461 acgctacatc gaggcgttgt ctgccattgt tgaaaccggg cagaaaatgc tggtagcggg
    32521 cgaaagtgcg ctggatgtgg tgacggaagc ggtgcgtctg ctggaagagt gtccactgtt
    32581 taacgccgga attggcgctg tctttacgcg tgatgaaacc catgaactgg acgcctgtgt
    32641 gatggatggt aacaccctga aagccggtgc ggtggcgggc gttagtcatc tgcgtaatcc
    32701 ggttcttgcc gcccgactgg tgatggagca aagcccgcat gtgatgatga ttggcgaagg
    32761 ggcagaaaat tttgcgtttg ctcatggcat ggagtgcgtc tcgccggaga ttttctccac
    32821 gcctttgcgt tatgaacaac tactggcagc gcgcgaggaa ggggcaacag tcctcgacca
    32881 tagcggtgcg ccactggatg aaaaacaaaa aatgggcacc gtaggggccg tggcgttgga
    32941 tttagacggc aatctggcgg cagccacgtc cacgggcgga atgaccaata aattacccgg
    33001 acgagttggc gatagtccct tagtgggggc cggatgctat gccaataacg ccagtgtggc
    33061 ggtttcctgt accggtacgg gcgaagtctt catccgcgcg ctggcggcat atgatatcgc
    33121 cgcgttaatg gattacggcg gattaagtct cgcggaagcc tgcgagcggg tagtaatgga
    33181 aaaactccct gcgcttggcg gtagcggtgg cttaatcgct atcgaccatg aagggaatgt
    33241 cgcgctaccg tttaacaccg aaggaatgta tcgcgcctgg ggctacgcag gcgatacgcc
    33301 aaccaccggt atctaccgtg aaaaagggga caccgttgcc acacagtgat gaacttgatg
    33361 ccggtgatgt gctggcggtt gaaaatctga atattgcctt tatgcaggag cagcacaaaa
    33421 tagctgcggt ccgcaatctc tcttttagcc tgcaacgtgg tgagacgctg gcaattgttg
    33481 gcgaatccgg ctccggtaag tcagtgactg cgctggcatt gatgcgcctg ttggaacagg
    33541 cgggcggttt agtgcagtgc gataaaatgc tgttgcagcg gcgcagtcgc gaagtaattg
    33601 aacttagcga gcagagtgct gcacaaatgc gccatgtgcg cggggcggat atggcgatga
    33661 tatttcagga gccgatgaca tcgctgaacc cggtatttac tgtgggtgaa cagattgctg
    33721 aatctattcg tctgcatcag aacgccagtc gtgaagaagc gatggtcgag gcgaagcgga
    33781 tgctggatca ggtacgcatt ccggaggcac aaaccattct ttcacgttat ccgcatcaac
    33841 tctctggcgg gatgcgccag cgagtgatga ttgcgatggc gctgtcatgc cgcccggcgg
    33901 tgctgattgc cgatgagcca accaccgcgt tggatgtcac tattcaggcg cagatcctgc
    33961 aattaatcaa agtattgcaa aaagagatgt cgatgggcgt tatctttatc actcacgata
    34021 tgggcgtggt ggcagagatt gccgatcggg tactggtgat gtatcagggc gaggcggtgg
    34081 aaacgggtag cgtcgaacag atttttcatg caccgcaaca tccttatacc cgtgcgctgt
    34141 tagctgctgt tccgcaactt ggtgcgatga aagggttaga ttatccccga cgtttcccat
    34201 tgatatcgct tgaacatcca gcgaaacagg agccacccat cgagcagaaa acggtggtgg
    34261 atggcgaacc tgttttacgg gtgcgtaatc tggtcacccg tttccctttg cgcagcggtt
    34321 tgttgaatcg cgtaacgcgg gaagtgcatg ccgttgagaa agtcagtttt gatctctggc
    34381 ctggtgaaac gctatcgctg gtgggagagt ctggcagcgg taaatccacc accgggcggg
    34441 cgttgctgcg cctggtcgaa tcgcagggcg gcgaaattat ctttaacggt cagcgaatcg
    34501 ataccttgtc acccggcaaa cttcaggcat tgcgccgcga tattcagttt atttttcagg
    34561 acccttacgc ttcgctggac ccgcgtcaga ccatcggtga ttcgattctc gaaccgctgc
    34621 gcgtacacgg tttattgcca ggtaaagaag cggctgcacg cgttgcgtgg ttgctggagc
    34681 gcgtgggtct gttaccggaa catgcctggc gttaccctca tgagttttcc ggcggtcagc
    34741 gccagcgcat ctgcattgct cgcgcgttgg cattgaatcc aaaagtgatc attgccgacg
    34801 aagccgtctc ggcgctggat gtttctattc gcgggcagat tatcaacttg ttgctcgatc
    34861 tccagcgtga tttcggcatt gcgtatctgt ttatctccca cgatatggcc gtggtagagc
    34921 ggattagtca tcgtgtggcg gtgatgtatc tcgggcaaat tgttgaaatt ggtccgcggc
    34981 gcgcggtctt tgaaaatccg cagcaccctt atacgcgtaa attactggcg gcagttccgg
    35041 tcgctgaacc gtcccgacaa cgaccgcagc gtgtactgct gtcggacgat cttcccagca
    35101 atattcatct gcgtggcgaa gaggttgcag ccgtctcgtt gcaatgcgtc gggccggggc
    35161 attacgtcgc acaaccacaa tcagaatacg cattcatgcg tagataacat tcaggcggag
    35221 aataaaatgg caagagctgt acaccggagt gggttagtgg cgctgggcat tgcgacagcg
    35281 ttgatggcat cttgtgcatt cgctgccaaa gaggtggtgg tggcggtggg atcgaacttc
    35341 accacgctcg atccgtatga cgcaaatgac acgttatctc aggccgtagc gaaatcgttt
    35401 taccaggggc tgttcggtct ggataaagag atgaaactga aaaacgtgct ggcggagagt
    35461 tacaccgttt ccgatgacgg ccttacttac accgtgaaat tgcgggaagg cattaaattc
    35521 caggatggca ccgatttcaa cgcagcggcg gtgaaagcga atctggaccg ggccagcgat
    35581 ccggcgaatc atcttaaacg ctataacctg tataagaata ttgccaaaac ggaagcgatc
    35641 gatccgacaa cggtaaagat taccctcaaa cagccattct cagcgtttat taatattctt
    35701 gcccatccgg cgaccgcgat gatttcaccg gcagcgctgg aaaaatatgg caaggagatt
    35761 ggttttcatc cggtgggaac cgggccgtat gaactggata cctggaatca gaccgatttt
    35821 gtgaaggtga aaaaattcgc gggttactgg cagccaggat tgcccaaact ggacagcata
    35881 acctggcgtc cggtggcgga taacaacacc cgcgcggcaa tgctgcaaac cggtgaagcg
    35941 cagtttgctt tccccattcc ctacgagcag gccgcactgc tggagaaaaa caaaaatatc
    36001 gagttgatgg ccagtccgtc aattatgcag cgttatatca gtatgaacgt gacgcaaaag
    36061 ccgttcgata acccgaaggt ccgtgaggcg ctgaattacg ccattaaccg cccggcgctg
    36121 gtgaaagtgg ccttcgcggg ctatgcaacg ccagctactg gtgtggtacc gccgagtatc
    36181 gcctacgcgc aaagttataa accgtggcct tacgatccag tgaaagcgcg cgaattactg
    36241 aaagaggcgg gatatcacaa cggtttcagt accacgctgt ggtcgtcaca taaccacagc
    36301 accgcgcaga aagtgctgca atttacccag cagcagttag cgcaggttgg gattaaagcc
    36361 caggtgactg cgatggatgc cggacagcgg gcggcagaag ttgaaggtaa agggcaaaaa
    36421 gagagcggcg tgcggatgtt ctacactggc tggtcggctt caaccggcga agccgactgg
    36481 gctctatcgc cgctgtttgc ctcgcagaac tggccaccaa cgctgtttaa taccgcgttt
    36541 tacagcaata aacaggtgga tgacttcctg gctcaggcac tgaaaactaa tgatccggcg
    36601 gaaaagaccc gcttgtataa ggcggcgcag gatatcatct ggcaagagtc gccgtggatc
    36661 ccgctggtgg tagaaaaact ggtgtcggca cacagtaaaa acctgaccgg tttttggagc
    36721 atgccagaca ccggcttcag ctttgaagac gcggatttgc aataagcaac gcagggagtg
    36781 gcatgcttaa ttacgttatc aaacgcttac tggggttgat tccgacgctg tttatcgtct
    36841 cggtgctggt gtttttattt gtccacatgc tgcccggcga tccggcgcga ttaattgccg
    36901 ggcccgaagc tgatgcgcag gttatagaac tggtgcgtca gcagctgggg ttggatcagc
    36961 cgctatatca ccagttctgg cactatatca gcaatgctgt gcagggggat tttggcctgt
    37021 cgatggtgtc gcgtcgtccg gttgccgatg agattgccag ccgctttatg ccaacgctgt
    37081 ggctgaccat aaccagtatg gtctgggcgg tgatatttgg tatggcggcg ggaattatcg
    37141 ccgccgtctg gcgtaaccgt tggccggatc gattgagtat gaccattgcg gtgtcgggga
    37201 tctcgtttcc ggcatttgct ctggggatgc ttttaattca ggtattctcc gttgaactgg
    37261 gctggctgcc taccgtggga gcagacagtt ggcagcacta cattttaccc tccctgacgc
    37321 tcggcgcggc agtggccgcc gtgatggcgc gctttacccg cgcgtcgttt gtcgacgttt
    37381 taagcgaaga ttatatgcgt accgcgaggg cgaaaggggt gagcgaaacc tgggttgtcc
    37441 tcaaacacgg gctacgcaac gcgatgatcc cggtagtgac catgatgggc ttacagtttg
    37501 gcttcttgct cggtggttcc atcgtggtgg agaaagtttt caactggccc ggacttggac
    37561 gcttactcgt tgactccgta gaaatgcgcg attacccggt gattcaggcg gaaattctgc
    37621 ttttctcgct ggaatttatt cttatcaact tagtggtgga tgtgctttac gccgccatta
    37681 acccggctat caggtacaag taaggatgcg actatttaac tggcgacgtc agacggtgtt
    37741 aaacgccatg ccactggtca aacctgacca ggtacgtaca ccatggcatg aattctggcg
    37801 acgatttcgc cgtcagcata tggcgatgac cgccgcattg ttcgttattt tattgattgt
    37861 ggtggccatt tttgcacgct ggatcgctcc ctatgacgcc gaaaattatt ttgattatga
    37921 caatctgaat aacggacctt ctttgcagca ctggtttggt gtcgattcac tggggcgcga
    37981 cattttcagc cgtgtcctgg ttggtgcgca aatctcgctg gcggcgggtg tgtttgccgt
    38041 gtttatcggt gcggcgatcg ggacgttgct gggcttgctg gctggatatt atgaaggctg
    38101 gtgggatcgg ctgatcatgc gtatttgcga tgtgctgttt gccttcccgg gtattttact
    38161 ggcgatcgct gttgttgcgg tgttgggaag cggcattgct aacgtgatta tcgcagtcgc
    38221 cattttttcc atccccgcgt ttgcccggct ggtgcgcggc aacacgctgg tgttgaaaca
    38281 gcaaaccttt atcgagtcag cacgcagtat tggtgccagc gatatgacca ttttgttgcg
    38341 tcatatcttg cctgggaccg tctcttctat cgtggtgttt ttcaccatgc gcattggcac
    38401 ctcgattatc tctgccgcca gcctgtcatt tctcggcctc ggtgcgcagc cgccgacacc
    38461 agagtggggg gcaatgctca atgaggcacg agcggatatg gttatcgcgc cgcatgtcgc
    38521 tgtttttccg gccctggcta tttttctgac cgtactggcg ttcaatttgt tgggcgatgg
    38581 tttacgcgat gcgctggatc cgaaaattaa aggatagtta cgtttgaata ttgcttgaaa
    38641 gggtaatcac ctcacaggaa attattgccc taagcaagtg ttgtaacttt ctgctgattt
    38701 tgtagaatcg ggtaatttgg ttaaaaagcc gcagcaaggg actattttgc agcggcacag
    38761 cgttcagata gttattttgt taaatgtatt aacatgctga gtttatacga aaagataaag
    38821 ataaggctga taattttatt tttattggca gcactgtcat ttattggtct ttttttcatc
    38881 attaactatc aactggtatc ggaacgcgcg gtaaaacgtg ccgatagccg ctttgaactt
    38941 attcagaaaa acgttggcta tttctttaaa gatattgaac gttcggccct gacattaaag
    39001 gactcactgt atttattaaa aaatacagag gagattcaac gtgccgtgat tctgaaaatg
    39061 gaaatgatgc catttttaga ctcggtggga ctggtacttg atgataataa atattatctc
    39121 ttttcgcgga gggcgaatga taaaatcgtt gtttatcatc aggaacaagt aaatggaccg
    39181 cttgtcgacg agtcagggcg ggttattttt gccgatttta acccatcgaa acgaccgtgg
    39241 tcgatggctt cagatgactc taacaacagc tggaatccgg catacaattg ctttgatcgt
    39301 ccgggtaaaa aatgtatctc ttttacgcta cacatcaacg gcaaagatca tgatttgtta
    39361 gcggtagata aaattcatgt cgatttaaac tggcgatatc tgaacgagta tcttgatcac
    39421 atcagcgcta atgatgaagt tctattttta aagcaaggtc atgagatcat tgct
//
LOCUS       DAZKHK010000032        38538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKHK010000032 DAZKHK010000000
VERSION     DAZKHK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.032"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..322
                     /gene="bhsA"
                     /locus_tag="ACHOS5_001682"
                     /gene_synonym="ycfR"
     CDS             65..322
                     /gene="bhsA"
                     /locus_tag="ACHOS5_001682"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP9776504.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(404..1366)
                     /gene="ldtC"
                     /locus_tag="ACHOS5_001683"
     CDS             complement(404..1366)
                     /gene="ldtC"
                     /locus_tag="ACHOS5_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP9776505.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSADSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1509..4955)
                     /gene="mfd"
                     /locus_tag="ACHOS5_001684"
     CDS             complement(1509..4955)
                     /gene="mfd"
                     /locus_tag="ACHOS5_001684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9776506.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEEINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREGLGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVDLRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5083..6156)
                     /gene="ycfT"
                     /locus_tag="ACHOS5_001685"
     CDS             complement(5083..6156)
                     /gene="ycfT"
                     /locus_tag="ACHOS5_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9776507.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERNLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWWGMNSVSRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLAVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6418..7617
                     /gene="lolC"
                     /locus_tag="ACHOS5_001686"
     CDS             6418..7617
                     /gene="lolC"
                     /locus_tag="ACHOS5_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP9776508.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7610..8311
                     /gene="lolD"
                     /locus_tag="ACHOS5_001687"
     CDS             7610..8311
                     /gene="lolD"
                     /locus_tag="ACHOS5_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP9776509.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8311..9555
                     /gene="lolE"
                     /locus_tag="ACHOS5_001688"
     CDS             8311..9555
                     /gene="lolE"
                     /locus_tag="ACHOS5_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP9776510.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9584..10495
                     /gene="nagK"
                     /locus_tag="ACHOS5_001689"
     CDS             9584..10495
                     /gene="nagK"
                     /locus_tag="ACHOS5_001689"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP9776511.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10511..11332
                     /gene="cobB"
                     /locus_tag="ACHOS5_001690"
     CDS             10511..11332
                     /gene="cobB"
                     /locus_tag="ACHOS5_001690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP9776512.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11470..12255)
                     /locus_tag="ACHOS5_001691"
     CDS             complement(11470..12255)
                     /locus_tag="ACHOS5_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFP9776513.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVVVPSVNEASFRQNGKAMLAHAASSMSNVKGSVLLLFTTRE
                     PRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDLPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAVA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(12252..12713)
                     /gene="ymfA"
                     /locus_tag="ACHOS5_001692"
     CDS             complement(12252..12713)
                     /gene="ymfA"
                     /locus_tag="ACHOS5_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP9776514.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            complement(12771..13817)
                     /gene="potD"
                     /locus_tag="ACHOS5_001693"
     CDS             complement(12771..13817)
                     /gene="potD"
                     /locus_tag="ACHOS5_001693"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP9776515.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            complement(13814..14608)
                     /gene="potC"
                     /locus_tag="ACHOS5_001694"
     CDS             complement(13814..14608)
                     /gene="potC"
                     /locus_tag="ACHOS5_001694"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP9776516.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFIILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(14605..15462)
                     /gene="potB"
                     /locus_tag="ACHOS5_001695"
     CDS             complement(14605..15462)
                     /gene="potB"
                     /locus_tag="ACHOS5_001695"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP9776517.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(15446..16582)
                     /gene="potA"
                     /locus_tag="ACHOS5_001696"
     CDS             complement(15446..16582)
                     /gene="potA"
                     /locus_tag="ACHOS5_001696"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP9776518.1"
                     /translation="MGQSKKLNKQPNSLSPLVQLAGIRKCFDGKEVIPQLDLTIKNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            16832..18058
                     /gene="pepT"
                     /locus_tag="ACHOS5_001697"
     CDS             16832..18058
                     /gene="pepT"
                     /locus_tag="ACHOS5_001697"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9776519.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(18107..19228)
                     /gene="roxA"
                     /locus_tag="ACHOS5_001698"
                     /gene_synonym="ycfD"
     CDS             complement(18107..19228)
                     /gene="roxA"
                     /locus_tag="ACHOS5_001698"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP9776520.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(19304..20764)
                     /gene="phoQ"
                     /locus_tag="ACHOS5_001699"
     CDS             complement(19304..20764)
                     /gene="phoQ"
                     /locus_tag="ACHOS5_001699"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP9776521.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20764..21435)
                     /gene="phoP"
                     /locus_tag="ACHOS5_001700"
     CDS             complement(20764..21435)
                     /gene="phoP"
                     /locus_tag="ACHOS5_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP9776522.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(21605..22975)
                     /gene="purB"
                     /locus_tag="ACHOS5_001701"
     CDS             complement(21605..22975)
                     /gene="purB"
                     /locus_tag="ACHOS5_001701"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9776523.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(22979..23620)
                     /gene="hflD"
                     /locus_tag="ACHOS5_001702"
     CDS             complement(22979..23620)
                     /gene="hflD"
                     /locus_tag="ACHOS5_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP9776524.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23656..24762)
                     /gene="mnmA"
                     /locus_tag="ACHOS5_001703"
                     /gene_synonym="trmU"
     CDS             complement(23656..24762)
                     /gene="mnmA"
                     /locus_tag="ACHOS5_001703"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9776525.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(24816..25277)
                     /gene="nudJ"
                     /locus_tag="ACHOS5_001704"
     CDS             complement(24816..25277)
                     /gene="nudJ"
                     /locus_tag="ACHOS5_001704"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP9776526.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(25287..25940)
                     /gene="rluE"
                     /locus_tag="ACHOS5_001705"
     CDS             complement(25287..25940)
                     /gene="rluE"
                     /locus_tag="ACHOS5_001705"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP9776527.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGNYSLDNLANGEWRDATD"
     gene            26112..27362
                     /gene="icd"
                     /locus_tag="ACHOS5_001706"
     CDS             26112..27362
                     /gene="icd"
                     /locus_tag="ACHOS5_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP9776528.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(27476..28618)
                     /locus_tag="ACHOS5_001707"
     CDS             complement(27476..28618)
                     /locus_tag="ACHOS5_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9776529.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFESVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLSLKCDALNITLREVIS
                     QCRDAVVSKYLVHYRHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(28608..28844)
                     /locus_tag="ACHOS5_001708"
     CDS             complement(28608..28844)
                     /locus_tag="ACHOS5_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9776530.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(28984..29223)
                     /locus_tag="ACHOS5_001709"
     CDS             complement(28984..29223)
                     /locus_tag="ACHOS5_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9776531.1"
                     /translation="MFRVIYPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVSKVESERIAEEIDTAEHIKKLRAIRRK"
     gene            complement(29201..29533)
                     /locus_tag="ACHOS5_001710"
     CDS             complement(29201..29533)
                     /locus_tag="ACHOS5_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9776532.1"
                     /translation="MANLQLAVKSEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTITHPHFGDKPVKVFAIKVNISTEYQSAQHKV
                     KNVQSDLP"
     gene            complement(29533..29754)
                     /locus_tag="ACHOS5_001711"
     CDS             complement(29533..29754)
                     /locus_tag="ACHOS5_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9776533.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            complement(29853..30068)
                     /locus_tag="ACHOS5_001712"
     CDS             complement(29853..30068)
                     /locus_tag="ACHOS5_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9776534.1"
                     /translation="MTPQQENALRSIARQANYEIKKARQQFPDKNVDDICRSILKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(30145..30336)
                     /locus_tag="ACHOS5_001713"
     CDS             complement(30145..30336)
                     /locus_tag="ACHOS5_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9776535.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            complement(30309..30491)
                     /locus_tag="ACHOS5_001714"
     CDS             complement(30309..30491)
                     /locus_tag="ACHOS5_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9776536.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            complement(30488..31168)
                     /locus_tag="ACHOS5_001715"
     CDS             complement(30488..31168)
                     /locus_tag="ACHOS5_001715"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9776537.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(31165..31950)
                     /gene="bet"
                     /locus_tag="ACHOS5_001716"
     CDS             complement(31165..31950)
                     /gene="bet"
                     /locus_tag="ACHOS5_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9776538.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            complement(31956..32252)
                     /locus_tag="ACHOS5_001717"
     CDS             complement(31956..32252)
                     /locus_tag="ACHOS5_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9776539.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(32328..32534)
                     /locus_tag="ACHOS5_001718"
     CDS             complement(32328..32534)
                     /locus_tag="ACHOS5_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP9776540.1"
                     /translation="MIHQHYGTQTVNRGAVMPGMLVKHKDATWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNY"
     gene            complement(33005..33448)
                     /locus_tag="ACHOS5_001719"
     CDS             complement(33005..33448)
                     /locus_tag="ACHOS5_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002969635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HFP9776541.1"
                     /translation="MKSRHRIAPAIILFLLPFPIIISAYYLFRHMFPGMAFSEHRDAL
                     GGAIAAYSGTTIAILIAALTFLMGLGGRNIRKLKTYGYMTSIIIMYGLTFCELGVLFF
                     NGLFLLATSKNPILMLPSIAIGLSGTSLIHIGMLLMQLLNLSKHK"
     gene            complement(33463..34308)
                     /locus_tag="ACHOS5_001720"
     CDS             complement(33463..34308)
                     /locus_tag="ACHOS5_001720"
                     /inference="COORDINATES: protein motif:HMM:NF027299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rexA"
                     /protein_id="HFP9776542.1"
                     /translation="MKLNFYAVYIQNTSSLKKQPIDLLSFLTEQVINAKNHAQQIEDY
                     YVFAHHIINDSFILTKTFDSELVKKINRKTLSVDEIRNALSSDESLGFPSFLLIRKNI
                     IGYANTLFGPKTRDLAAYLKGKGAIPDGYSLFIEPLMRDITKDDALSMQFIGRTTVRV
                     ENGSKLLSPLLRVLGAQAVDEELLEGLEITVKPKRLRNIKSVAKGIISNVDEQHESIH
                     LKGKEQAADILTEYYLSDKGHIGANIYKSSNEDIASEMSTCYLRMKLIIMESYNRTFG
                     DELPL"
     gene            complement(34388..35077)
                     /locus_tag="ACHOS5_001721"
     CDS             complement(34388..35077)
                     /locus_tag="ACHOS5_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9776543.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            35182..35412
                     /locus_tag="ACHOS5_001722"
     CDS             35182..35412
                     /locus_tag="ACHOS5_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9776544.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            35482..36021
                     /locus_tag="ACHOS5_001723"
     CDS             35482..36021
                     /locus_tag="ACHOS5_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP9776545.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            36018..37037
                     /locus_tag="ACHOS5_001724"
     CDS             36018..37037
                     /locus_tag="ACHOS5_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9776546.1"
                     /translation="MTGSEMAKAGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKDVYPNQVNTKDKLTKDNIKPFSSENSGESSDQSENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWI
                     YGVEL"
     gene            37034..37735
                     /locus_tag="ACHOS5_001725"
     CDS             37034..37735
                     /locus_tag="ACHOS5_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9776547.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVMAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQIPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGARV"
     gene            37732..38019
                     /locus_tag="ACHOS5_001726"
     CDS             37732..38019
                     /locus_tag="ACHOS5_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9776548.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAMYGRG"
     gene            38305..>38538
                     /locus_tag="ACHOS5_001727"
     CDS             38305..>38538
                     /locus_tag="ACHOS5_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006176174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776549.1"
                     /translation="MITRNLGEETINSIIEEDNLLSEQVQVIDGCIVINIEYEYPIPL
                     NTCSTYQQILHWVWHLAEKTWMTQIVTRRFIEIA"
BASE COUNT         9542 a         9493 c         9726 g         9777 t
ORIGIN      
        1 tcatttagtt atcgatcgtt aagtaattgc ttgcgacgtc attcatctgc ataaggccac
       61 tattatgaaa aacgtaaaaa ccctcatcgc tgcggcgatt ttaagctcaa tgtcatttgc
      121 cagctttgcg gctgtcgaag ttcagtcaac gccagaaggc caacaaaaag tcggtactat
      181 cagtgctaac gcggggacaa atctgggatc gctggaagag cagctggcgc aaaaagcgga
      241 tgagatgggc gcaaaatctt tccgtattac ttctgtaacc ggtccgaata ccctccatgg
      301 aacagcagta atttataaat aagcattaac cctcattaat gcctgctact gctgattttt
      361 tccccgcgac atgccgtgtc gcggggattt ttttatccga gctttacaga gtttgtgggc
      421 tcacttgatg acggcggaca tccaccggca tcccggaacg gacatccatc acatgttgca
      481 tcacttcagc gtcagtttgt gctgcatctt taaatgattg cattgcgcta ttcagcgtaa
      541 ttggcagcag ctgcggatcg tcatcaatct tctctgacag cggctgatgt acttcaacca
      601 gacgcgcacc gtttggttcg gcagagactt taatcggggt gttgatgata ttcactttgg
      661 tgcctggggt gacctggcta aagagtgttt tgatatcgtc atcccgcaga cgaatacagc
      721 cagaacttac ccgcatgcca atgccgaaat cagcgttcgt accgtgaagc aaataaacgc
      781 cgccataggc cgccagacga atcgcatgat ggcccattgg gttatccggt ccagccggaa
      841 ctaccgcagg caactcaatt ccctgtgcct tataccgcgc gcgaatgttt gccgttggcg
      901 tccaggttgg gtttgcacgc ttgtcagaaa cagtggtcac cattgtcggt gtcagcgtgt
      961 caccacctaa ctgaccaatg ccgatgggat atacggttac cgaatttttt cccggcggat
     1021 aataatagag acgcagctcc gcaatgttga tcacaatgcc ttcgcgcggc gcgtctggaa
     1081 gtagggtttg caacgggatc gttaacacgc tacccgcgcg cggtacgtaa ggatcaacgc
     1141 cggggttagc ctgtaacaga gcgagaaagc cgacgttata ttttttggcg atcgcttcca
     1201 gagaaccacc gtcattttcc accacatgaa atttgttttc accaaccaga cgactgtccg
     1261 ctgacggcag cggccaggtg ttggcttttg ccggtagcgc cagcgccacg gcagcggcga
     1321 acgtaaaaaa cgttagccag cgagaaaaac gcgttttgat catcaccaaa gtccataata
     1381 aatataaggt tattgtaata aaaaagtaag tctgaattat ggagggtgac agtgtcggat
     1441 aatgcaggga agtgcaaaga atttgtaaat attgcagatg ggggcgcaga gacgcccccg
     1501 atttaccatc aagcgatcgc gttctcttcc agttcacgca taaactggcg tacccattcg
     1561 atacgcgttt tccgctcgct caaatcctga ataaacttca gacgcgtcgg accatcaagg
     1621 cggtaatgct gcggctgttt ttgcagcaaa ccaatcaacc aggctggatt aacgtgattc
     1681 ttctcggcaa attcgatcac gccgcctttc tcattacctt ccagcttcct gatccccagt
     1741 ttctgcgctt gctggcgcag acgggcaata tccagcaggg tacgcgccgg atccggcagc
     1801 aggccgaagc gatcgataag ctcgactttg atctcttcca gttcgttttc cgttttggcg
     1861 ctggcaatac gtttgtagaa cgacagacgc gtgttcacgt cagggatgaa atcatctggc
     1921 aatagcgacg gcatccgcag gtcgacttct gtttgctggc tggtgagatc ttccagcgac
     1981 ggctcgcgtc cggctttcag cgcatcgacg gcgttttcca gcaactccat atacagcgag
     2041 aaaccgatgg tttccattga gccgctttgt tcttcgccaa gcagttcacc tgcgccgcgg
     2101 atctccagat cgtgcgttgc cagcgcaaaa cctgcaccga gatcttccag cgaggcaatc
     2161 gcttcaagac gtttttgcgc atcggtagtc atcgcttttg gatgcggcgt cagtagccat
     2221 gcatatgcct ggtggtgaga acgcccgacg cgaccgcgca actggtgcaa ctgtgccaga
     2281 ccgaagtgat ccgcgcgttc aatgataatg gtgttggcgg taggaatgtc gatcccggtt
     2341 tcgataatgg tggtacaaac cagcacgttg aaacgttgat gatggaaatc attcatcacc
     2401 cgttccagtt cgcgctcacg catttgcccg tgaccgatgg caatccgcgc ttcaggtacc
     2461 agttctgcca gccgttcggc ggctttctgg atgttttcca catcattgta gagataataa
     2521 acctgtcctc cgcgcaaaat ttcacgcagg atcgcctccc ggaccaccag actgtcatac
     2581 tcacggacaa aggttttaac tgccagacga cgggcgggcg gcgtggcgat aatcgacaga
     2641 tcgcgcattc cgctcattgc catattcagc gtacgtggga tcggcgttgc agtaagcgtc
     2701 agaatatcca cgttcgcgcg catcgcttta atgcgctctt tatgacgcac cccgaagcgg
     2761 tgttcttcat cgacaatcag caggcctaaa tctttaaact tgacgtcact ttgcagcagt
     2821 ttgtgcgtac cgatcagaat atcgattttc ccttccgcca cttccgcaag gatttgcgtc
     2881 tgctctttgg cgctgcggaa acgggagatc atttcgatac gtaccggcca gttggcgaaa
     2941 cggtcgcgga agttgtcgta atgctgctgc gcgagaaggg tggtaggcac cagcaccgcc
     3001 acctgtttgt ggttatctac cgccaggaaa gcggcgcgca tcgccacttc tgttttacca
     3061 aagccaacat cgccgcacac cagacgatcc attgccagcg gctgacacat gtcgctaagc
     3121 accgcattaa tggcttgagc ctgatccggc gtggtttcaa acgggaagct gtcgcagaat
     3181 aactgatact gctcacgatc gtgtttaaac gcgaagccct ctttggcggc gcgttgcgcg
     3241 tagatatcca gcaattccgc cgccacatca cgcacttttt ccgccgcttt ctgccgtgcg
     3301 cgtgaccagg catcgccacc aagtttatgc agtggtgcgt tttcttctgc gccacccgcg
     3361 taacggctaa tcagatgcag tgacgacacc ggaacataca gtttggcgtc gttggcatag
     3421 gtgagcatca aatactcgcc agtaatgccg ccagcttcga gcgtggtcat tccggcataa
     3481 cgaccgacgc cgtgctccag atggaccacc ggctgaccaa tatgcagctc cgcaaggtta
     3541 cggatcagtg tatcggggtt gatggcgcgg cgagaatcct gacgacgacg ggcaacgcgt
     3601 tcaccgagca gatcgctttc gcaaatcagc gccaggttac gcatcgtatc gacaaaacca
     3661 tgttcggcag cgccaatcat cagataacgc ccacggtcgc tggcttcatc aagacgcata
     3721 atgcgttgcg gagcaatttt aattcgcgcg agcagttcac ccagcccttc acggcgacct
     3781 tcactttcta ccgagaacac caccggaccg tcgaaagtct cgaggaactt acgcagcgca
     3841 tccagcggcg ctttttgttg tgcctgaatg gccaggtctg gcagtttctg gaaacctaaa
     3901 ttggcattcg ctgcttttgt cggtaaatgt tcagttttta gctgcacacg cggccagttt
     3961 ttcagctctg aaaagagctc atccacccgc agccagagcg attgtggtgg caacagtggg
     4021 cgcattgggt cgacgccgcg attctcaaaa cgcgccagcg tgtcagcctg gaaacgttcg
     4081 gcactgtttt ccagatcgcc agtattcacc aacagcgtat tggcagggaa ataactgaac
     4141 agtggcggca gcggttcgct gaagaatagc ggctgccagt actcgatccc ggcaggtaat
     4201 gtgcctttac tcacttgctg ataaatatgt tccgggtcgc gcttcacttc gaaggtatca
     4261 cgccactggc tgcggaacag ttcaattgcc gctttatcgg tcggaaattc gtgcgcggga
     4321 agcagattga tctcttctac ttcctccagc gtgcgctggc tatcgacgtc aaacacccgc
     4381 aggctgtcga tttcatcatc aaagaaatca agacgataag gcagctcact ccccatcggg
     4441 aagagatcca gcaacgcgcc gcgcgtggcg tattcgccgt gctccatcac ctggtcaaca
     4501 tggcgataac cggcgctgtc cagttgggtt cgtaatgcat ctcgtgacag gcgctgacct
     4561 tttttcatca ccagcgcatg accgtggaga aaactgtgcg ggcaaacgcg ctgcataagc
     4621 gtattcaccg gaacaatcag tacgccacgc tgcatcgtcg gtagctggta aagggtggaa
     4681 aggcgcgagg agataatgtc ctgatgaggc gaaaaactgt cgtaagggag ggtttcccag
     4741 tccgccaggt tcatcaccat ctgatcggtg aactggctga tttcatcatg caaacgcaga
     4801 gcattttgca tatctggcgc aataagcacc actggtccgg cgtgacgttc ggcaatttcc
     4861 gctaccagcg tcgcgcaggc agcgccagtt aactcgccca gcagacgctg ctcacccgct
     4921 ttgacgggca gcgtataacg atattgttca ggcataacgg ttgtcagatt ctcgttagga
     4981 tatgcctcaa catatggggg catatctctg ataagcaatg ctgttcatta tccgtaatcg
     5041 acatggttta gcaaattgaa tcgccccggc tggggcgatg gcttagcggg agtaactgac
     5101 ggccgcgggc agagaaggcg gggagaacaa cagatcgctg aatgcgcgct gtgaaagttt
     5161 tcttaccagc aagcccgcaa cagtacagat gaacaaacta acaaaggggt aaaccagcag
     5221 gagtgtcagt tcgacttgcg gcgaccagcg tgctgcgttc atttgcgcaa gcagagttaa
     5281 gctgaatatt tcaaccagaa tgcgatgggt ggtgtagata gcaatggtgt tggaaccaat
     5341 cacattcagc aggctggtgg agcgcatacc gaaacgttgt tcgtactgat aaaacagctt
     5401 catgatcacc acaatcgata ccagcgacaa cagcagcgag atattaaaca accaggcacc
     5461 gaccgccaga acagccagca aagaagccat cagcagatgg cggcgcaacg gtaccgcttt
     5521 aacacaggtc attaatgttg cgccaaacca tgcgccaagg ctgtaataag gcaaattgcg
     5581 gctcacactg ttcattcccc accacggtgt gggtacgaaa ttaacagcca cactcatcag
     5641 tacaaacaga acaaataacg gcagggccag gcggttaaaa attttacata tcacgaaata
     5701 gacaattaac gcatacagat accacaagct ggtgctggcg gtgatcatcc cgtgcaggaa
     5761 ctcaccggtg gaatcggcat aagcggcatt ggaggcattg cttaaattgc gctcaggtgc
     5821 cagccactga tttagtgcac ttagcgccag ccactgcacc acgccccaaa gcgccagcac
     5881 ccagaagatg ttccagatgc gtttatcgag acaatttccc cacggtacgc tgtcgatata
     5941 gcggcgaatc aaatagccag agataaagaa aaaaaccggc atacgaaagg gtgcaaggta
     6001 aagattgaaa tagatccagc atttgctcag gacttctgat aacggatgct ggaaagtggt
     6061 cagatgcgga taaaaggtaa tgaccgagtg ataaatcacc accagacaaa tacataaccc
     6121 tttgatctgg ttaatccata gctctttttg tttcatcagg acaacacgct ctttttattt
     6181 aacatgctgt taatcctgcg gggcgagttg cgtggtgtaa tgcgtatctg tctgtatttg
     6241 gttatttttc agataaagat cagcccacaa ggccgatgag aaaatgaggc cctttagccg
     6301 attgatttac gggggctttt cagattagcc ctgacgatca cttacagttc agacgtttac
     6361 ccatcccgct ttcgcttata tactcgtgtc tttgctaaca gcaaccagac ggatttcatg
     6421 taccaacctg tcgctctatt tattggcctg cgttacatgc gtgggcgtgc agcggatcgc
     6481 ttcggtcgtt tcgtctcttg gctttctacc atcggcatta ccctcggggt gatggcgctg
     6541 gtcacagtat tgtcagtgat gaacggcttt gagcgcgagc tgcaaaacaa catccttggc
     6601 ctgatgccac aggcaattct ctcttctgag catggctctc ttaacccgca gcaactcccg
     6661 gaaacggccg tcaaactgga cggcgttaat cgcgtcgcac ctattactac cggtgatgtg
     6721 gtactgcaaa gcgcgcgcag cgtggcggtc ggggtgatgc tgggtatcga cccggcgcaa
     6781 aaagatccac taacgccgta tctggtcaat gtgaaacaaa ctgacctcga gccggggaaa
     6841 tataatgtca tcctcggtga acagcttgcc tcacagttgg gcgtcaatcg cggtgatcaa
     6901 atccgcgtga tggtgccatc tgccagccag ttcacgccga tggggcgtat tccaagtcag
     6961 cgcctgttca atgtgattgg cactttcgcc gccaacagtg aagtcgatgg ctatgaaatg
     7021 ctggtgaata ttgaggatgc ctcgcgtctg atgcgttatc cggcaggcaa tattaccggc
     7081 tggcgtttgt ggctggatga gccgctgaaa gtcgactcat taagtcagca aaaactgcct
     7141 gaaggcagca aatggcagga ctggcgtgac cgtaaaggcg agctgttcca ggccgtacgc
     7201 atggaaaaaa atatgatggg cttgctgctg agcctgattg tcgccgttgc ggcgtttaac
     7261 attattacct cgctgggact gatggtgatg gagaagcagg gcgaagtggc cattcttcag
     7321 actcaggggc tgactccgcg tcaaatcatg atggtcttta tggtgcaagg ggccagcgct
     7381 gggattatcg gtgcgatcct cggagcggcg cttggcgcac tgcttgccag ccagttgaat
     7441 aatctgatgc cgataatcgg cgtcctgctt gatggcgcgg cgctgccggt cgctatcgag
     7501 cctttacagg tcattgttat tgcgctggtg gcgatggcta tcgcgctgct gtctacgctt
     7561 tacccttcct ggcgcgctgc cgccactcaa cccgctgagg ctttacgtta tgaataagat
     7621 cctgttgcaa tgcgacaacc tgtgcaaacg ctatcaggaa ggcagtgtgc aaaccgatgt
     7681 actgcacaat gtcagtttca gcgtgggcga aggtgaaatg atggcgatcg tcggcagctc
     7741 tggttccggt aaaagcacct tgctgcacct gttaggcggg ctggatacgc caacctccgg
     7801 cgatgtgatc tttaacggtc aaccgatgag caaactgtcg tcggcggcca aagcggaact
     7861 gcgtaaccag aagctgggat ttatttatca gtttcaccac ctgttgccgg attttaccgc
     7921 cctggaaaac gtggctatgc cgctgttgat tggcaagaaa aagcccgctg aaatcaacag
     7981 ccgtgcgctg gagatgttaa aagcggtggg gctggagcat cgtgcgaatc accgcccatc
     8041 tgaactttct ggcggcgaac gccagcgtgt ggcgattgcc cgtgcgctgg tcaataaccc
     8101 gcggctggta ctggcggatg aacctactgg taacctcgat gcacgtaacg ccgacagcat
     8161 cttccagttg cttggggaat tgaatcgctt gcagggcacc gccttcctgg tggttactca
     8221 cgacctgcaa ctggcgaaac gtatgagccg ccaactggaa atgcgtgatg gacgtttgac
     8281 ggcggaactg agcctgatgg gggcggagta atggcgatgc ctttatcgtt attgattggc
     8341 ctgcgtttta gccgcggacg gcgacgcggc ggcatggtgt cgttgatctc cgtcatttct
     8401 accattggca ttgccctcgg cgtggcggta ttgatcgtcg gcttaagcgc gatgaacggc
     8461 tttgaacgcg aactgaataa ccgcattctg gcggtagtac cgcacggtga aattgaggcg
     8521 gtagatcagc cgtggaccaa ctggcaggaa gcactggata acgtgcagaa agtgccgggc
     8581 attgccgccg ctgcgccgta tatcaatttc accgggctgg tggagagcgg cgcgaatctg
     8641 cgcgcaatcc aggtgaaggg cgttaacccg caacaggaac agcgtctgag cgcattaccc
     8701 tcgtttgttc agggggatgc ctggcgcaat tttaaagcgg gcgaacagca aattatcatc
     8761 ggcaaaggcg tggcggatgc gctgaaagtg aagcagggcg attgggtgtc gattatgatc
     8821 cccaactcga atcccgagca taaactgatg cagccaaaac gtgtgcgttt gcacattgcc
     8881 ggtattttgc agttgagtgg ccaactcgat cacagttttg ccatgatccc gctggccgat
     8941 gctcagcaat atcttgatat gggttccagc gtgtcaggta ttgcccttaa aatgacggat
     9001 gttttcaacg ccaataagct ggtacgcgat gcgggtgaag tgaccaacag ctatgtttat
     9061 attaaaagct ggattggtac ttacggctat atgtatcgcg atatccagat gatccgcgcc
     9121 attatgtatc tggcgatggt gctggtgatt ggcgtggcct gtttcaacat cgtctccacc
     9181 ttagtgatgg cggtgaaaga caagagtggc gatatcgcag tattaagaac gctgggggcg
     9241 aaagatggtt taattcgcgc catctttgtc tggtatggat tgctggcagg gctgttcggc
     9301 agcctgtgtg gtgtggttat cggcgtggtg gtttcactgc aacttacccc gattattgag
     9361 tggattgaaa agttgatcgg tcatcagttc ctctccagcg atatctattt tattgacttc
     9421 ttgccatcgg aattgcactg gctggacgtc ttctacgtac tggtcacagc attgttgctg
     9481 agtcttttgg caagttggta tccggcgcgg cgcgccagta atattgaccc tgcgcgagtc
     9541 ctgagcggcc agtaaaggca gtacattaaa acaaggagcg gcaatgtatt acgggtttga
     9601 tattggtgga acaaaaattg cgctaggcgt gtttgatagc ggtcggcagt tgcagtggga
     9661 aaagcgggtg ccgacaccgc gtgacagcta tgacgcattt ttagatgcag tgtgcgagct
     9721 ggtagccgaa gctgaccggc gttttggctg taaaggttct gtcggcatcg gtattccggg
     9781 aatgccggaa acagaagatg gtacgctgta tgccgccaat gtccctgctg ccagcggtaa
     9841 accgctgcgt gccgacctga gcgcacgtct tgatcgcgat gtacgccttg ataacgatgc
     9901 caactgcttt gccctttctg aagcatggga tgatgaattt actcaatatc cactggtgat
     9961 ggggttgatt ctcggcaccg gcgttggcgg cgggctgatt ttcaacggta aaccaattac
    10021 cggtaaaagc tatattaccg gcgagtttgg ccatatgcgt ctgccggttg atgcgttaac
    10081 catgatgggg ctggatttcc cgttacgccg ctgcggctgt ggtcagcatg gctgcattga
    10141 aaattatctg tctggtcgcg gttttgcgtg gctgtatcaa cactattatc atcaaccgtt
    10201 gcaggctcct gagatcattg cgctttatga tcaaggcgat gagcaggcaa gggcgcacgt
    10261 tgagcgttat ctggatttat tagcggtttg tctgggaaat atcctgacca ttgttgaccc
    10321 tgacctggtc gtcattggtg gtggcttatc gaatttcccg gcaatcacaa cgcaactggc
    10381 ggaaaggttg cctcgtcatc tcttacctgt agctcgtgtt ccgcgcattg aacgcgcgcg
    10441 gcacggtgat gcggggggaa tgcgtggtgc ggccttccta catctaaccg attaatcaac
    10501 agaggttgct atgctgtcgc gtcggggtca tcggttaagt cgttttcgta aaaataaacg
    10561 ccgcctgcgc gagcgtttgc gtcagcgtat ttttttcaga gataaagtgg tgccggaagc
    10621 aatggaaaaa ccaagagtac tcgtactgac aggggcaggg atttctgcgg aatcaggtat
    10681 tcgtaccttt cgcgccgcag atggcctgtg ggaagaacat cgggttgaag atgtggcaac
    10741 gccggaaggt tttgatcgcg atcctgaact ggtgcaaacg ttttataacg cccgtcgtcg
    10801 acagctgcag cagccagaaa ttcagcctaa cgctgcacat cttgcgctgg ctaaactgca
    10861 agatgctctc ggcgatcgct ttttgctggt gacgcagaat atcgacaatc tgcatgaacg
    10921 cgcaggtaat accaatgtca ttcatatgca tggggaactg ctgaaagtac gttgttcgca
    10981 aagtggccag gtcctcgact ggaccggaga cgttacgcca gaagataaat gccattgttg
    11041 ccagttcccg gcacccttgc gcccgcacgt agtgtggttt ggcgaaatgc cactcggtat
    11101 ggatgaaatt tatatggcgt tgtcgatggc cgatattttc attgccattg gtacttcggg
    11161 gcatgtttat ccggcggctg ggtttgttca cgaagcgaaa ctgcatggcg cgcacaccgt
    11221 ggaactgaat cttgaaccga gtcaggttgg taatgaattt gccgagaaat attacggtcc
    11281 ggcaagccag gtggtgccag agtttgttga aaagttgctg aagggattat aaagcgggaa
    11341 aaattgcctg atgcgctacg cttgtcaggc ctgtgaaggg gcgcaatcaa ttgaatttgc
    11401 gggttttgtg ggccggataa ggcgttcgcg ccgcatccgg cataatacat gcgaacttta
    11461 ctgcctcttt taccgtttac tcgtccatag taacgcggca ataaacgtac agacaccaat
    11521 cagccctgcc cagagtggcg atattctggt aatcgcaaag ctgttatcga tatacaaaca
    11581 taaagcctga taacccatat tggcaacagc catcgccact gcaaatttta acgcataata
    11641 aaggcgcgtt gtgtagttaa aaaatgcagc aaagacgatg aatgcaactg cccaggcaaa
    11701 aaggcctaat gtcgtggtac tgccgggaag atcattctcc tgctgttgtc cgggatgcgg
    11761 ataatatgtc agtggcggcg tcggttgttt taatatctgt gcttgtaaaa cagccaatgc
    11821 attattgatt ccctgatacc agagatctgc atgtaaataa gccagagtgt gattttctac
    11881 cagaaggccg agatgtttat cgtccatgct gctttcaacc tggccgttgg ttatcataat
    11941 taaccgtggc tcgcgggtag taaaaagtaa cagaacactt ccttttacat tactcattga
    12001 agatgccgca tgcgccagca tcgctttgcc attttgcctg aagcttgctt cattaacgct
    12061 aggtacaacc acgataaacg gcgcaatccc ggtttgttga cgaaaaagtt cggctttacg
    12121 cgcgatctct tcggcatccg gtacagtacc ggtaacatcc gtaaaaagag tcttcattat
    12181 tggaatcaat ggcttactgg ttaccgtcag aggcaggagt atcagtaaac tgagcaatat
    12241 tatgattttt ttcatgggcg ggacttccgt ttcctgcgtg tcttttttga ggagggcata
    12301 ctgattaata aaccgacggc gataaaaata acgcccataa tgccgataaa cccccattta
    12361 aaaaaattaa cccacgggtt atttatctca gccttttcag gatgtcctgg agaataataa
    12421 acattaactt tatcgccttc gctgtcttca taaaaatcgc tgccaatact cgaattgaaa
    12481 attagcgtgt aatctggcgt cggacgaaag gcaacgacgg gataccacga accattttta
    12541 cccgtgcgat gagaatgact ggagtgccag acggtatcaa caatgacgcc ttcagtatga
    12601 atcgcgtctt tgctataggt gaactggtta taaaagataa caacagaaat gaggatcagt
    12661 accagaccga taccgaagaa tatacgaaag aaaactctac tgtcctggct cattagccac
    12721 atccttgcta aataaaaaac gggcggtaat accaccgccc gcttgctgaa ttaacgtcct
    12781 gctttcagct tctgataata ctcttcataa atgctgctgg cggagcctac gtcattctgc
    12841 cattcaccat ttttaatggt ttcagcatcc gggtagagcg ttttatcgtt cgccacttcc
    12901 ggacttaaca gcttacgcgc cgcaaggttt ggcgttggat aaccgatagt ttcagcaacc
    12961 tgttttgcca catccgggcg cagcaggaag ttgatcaatt tcagcgcgcc ttctttgttt
    13021 ttggcatttg ccgggatcgc caggctgtcc atccagaaaa tgccgccttc tttcggccat
    13081 accacatcta ttggcgtacc cgcctggcgt gcaacgaaag cagaaccgtt ccagatcatg
    13141 ccgaggttaa cttcgccttc catatacggg ttagcggggt tatcggagtt aaacgccgcg
    13201 acgtttggca tcagtttttt cagctcgttg tatgcagctt caatctcttt cggatcggtg
    13261 gtgttaccgg agtagcccag cttacgcagc gccatctgga acacttcacg ggcatcgtcg
    13321 gtcagcagca ggctgccttt gtactcaggc ttccacagat cggcccagct ggtgacagat
    13381 ttcggatcca ccgcatcacc gttaacgcca atcgccgttg caccccagat atacggaatg
    13441 gaatagtcgt tattcgggtc aaacggcttg ttgagcatgt ctggatcgag gttgctgaaa
    13501 ttgcttaact tcgacttgtc gatcttctgg atcatccctt ctttacgcat tttatcgacg
    13561 taataggttg aaggaaccac caggtcatag gcaccgtctt tgtacgtttt cagcttcgcg
    13621 tacatggttt cgttcgactc gtaagtcgaa tagataacct taataccggt ttctttggtg
    13681 aactgttcaa gcagtcctgg cggcacgtac tcggtccagt tgtagaaata cagcgtgttg
    13741 ttgtcatcgg cgtgagcggc gctcatgccc agtgccagag cacccgccgc gagcaggtgg
    13801 cgtgaccatt ttttcattta acgtcccctg tgttaccttt cgttttatca cgagcaataa
    13861 gctggctggc aataaccatc accagcgaca gcaccagtaa tatggttgcc agcgcgttaa
    13921 cttccggcga tacgccgact ttgaccatcg aataaatttt taacggcaga atttcatagc
    13981 ttggtccggt gacaaacgac gaaactacca catcgtccat cgacagggta aagcttagca
    14041 cccagcccgc cgccaccgct ggcattgcca gtggcaggat gatttttcgc agaatgataa
    14101 attcactggc accgagatct ttcgccgctt ccagcattcg cacgtcaaaa cctttcaggc
    14161 gcgaatacac cgtcaccacc acaaacggca ggcagaaggt gatatgcgag aacagcagcg
    14221 accagaagcc aagctgaata cccagcagca taaacagcac cagcagagaa atcgccatca
    14281 cgatatctgg cgacatcatc accacaaaca gcattccgct aacgaacggc ttaccacgaa
    14341 agcgatagcg gtacagcgca actgccgtca gtgaaccgat aagcgtggca aacgtcgccg
    14401 aaaacaccgc catcgtcagt gaatgctgcg ctgcctgtaa caggctgtcg ttgttcatca
    14461 gcaggctata ccatttggtg gtaaaaccct gccagttgat gccaaagcgc gagctgttaa
    14521 aggagttcac aatcaaaata atgattggga tatacaggta cgcgtagata gcggtcataa
    14581 aaccgccgcg aagcagtcga ccgatcattc gagttccacc ttcttattca gcagacgaga
    14641 agcgcgccag taaaccagca gcatcaggcc cattacgata gtcagcgtaa tgctggtggc
    14701 tgcaccaaac ggccagtcac gaatattgag gaactgtacc ttgatgacgt taccgatcag
    14761 caggtttttc gcaccgccca tcaggtcgga cacatagaac aggcccatcg ctggcagcat
    14821 caccagcaga catccggcaa taattcccgg catcgtcagt gggataatga tgcggataaa
    14881 ggtctgcaac ttgctggcac cgagatcgcg cgccgcctca agcaacggct tatccagttt
    14941 ttcgatactg gagtacagcg gcatcaccat aaacggcagc agaatgtaaa ccagaccgat
    15001 aatcaccgca ctaggcgtga acatgatgcg aattggtgta tcgataacgc ccagccagag
    15061 caaaaactcg ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt aaatacgaat
    15121 caatgagttg gtccagaacg gaacaatcag cagaaacagc agcagcggac gcaccttgtg
    15181 tggcagcttc gccagaaacc aggcaaaagg atagcccagc accaggcagg cgagggtggc
    15241 gatcagcgcc atattcagcg agtgcaatag cacttcaaaa tagagcggat cgagcagacg
    15301 cgtgtagtta tccagcgtaa agaccatttt gacgaaactg gcgtcgtcgc gggtcaaaaa
    15361 gctggtgcca atgatcatca ggttgggcag aaagacaaac aacacaagcc aaccgacaat
    15421 agtgacaatc actacattct ggaacttact tgtgttcttc atcagccagt acgacctccc
    15481 agctttctac ccaattaatg gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt
    15541 cttcattgaa gaattcgctg accatcacca tcttgccatt ttccagctca acaaccgact
    15601 ccagcgtcat gcctttgtag ttacgctcgc gaacgtaacc aatcagccct tcggcgtggt
    15661 tgtcgtcgtt aatctcttca acacgtaagt cttccgggcg cagcagaaca tgcagttttt
    15721 gccccggttc aacggcgaag ttaacgtaga tattacattc gcggccttca acgttggcgc
    15781 gtacgcgctg ctcgtcgaga cgttcgatga cagtggcgtt aaacatattg atttcgccaa
    15841 taaacccggc aacaaacagg tttttcggct cttcgtagat ttcacgcggc gtgccgtctt
    15901 gctcaatgcg accatcgcgc atcaccacga tcctgtccga catggtaagt gcttcttcct
    15961 ggtcgtgagt tacgaacacg aatgtaatgc caagcttacg ctgtaacgct ttcagctcgt
    16021 tctgcatttg cttgcgcagt ttgtaatcca gcgctgagag cgactcatcc agcaacaaca
    16081 gacgaggctt gttaaccacc gcgcgagcaa tggcgacgcg ttgctgttga ccaccagaga
    16141 gctgatgcgg tttgcgttga gcgaaggttt ccagctgcac catccgcagg gcttccatca
    16201 cgcggggcgt aatttcagca gcgggggttt tttgcatgcg caacccaaag gccacatttt
    16261 cgaacacggt catgtggggg aaaagtgcgt agctttggaa aacagtgttc acatagcggt
    16321 tttccgccgg aacgtgggtg atgtcctcgt tatccagcat gatgcgtccg gaatcaacag
    16381 tttccagacc tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa gggccaagca
    16441 gcgtgaggaa ctcgccattc ttgatagtca gatccaactg gggaatgacc tctttaccat
    16501 caaagcattt gcgaattccc gccaattgca ccagcggtga aagcgaattc ggttgtttat
    16561 tcaatttttt actctgtccc atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg
    16621 gttaaccacc ttggtgactc ttaatgaggg cggtaattct acggcaaacc gcttgaatcg
    16681 ccaatctttg ttgtgaatta ctggcttagc tttatattca ttaaggtaat gctgataaat
    16741 attcccgctt gcagggctaa aagtgacctg acgcaatatt tgtcttttct tgcttcttaa
    16801 taatgttgtc acaaaaagtg agggtgacta catggataaa ctacttgagc gatttttgaa
    16861 ctacgtgtct ctggataccc aatcaaaggc aggggtgaga caggttccca gcacggaagg
    16921 ccaatggaag ttattgcatc tgctgaaaga gcagctcgaa gagatggggc ttatcaatgt
    16981 gaccttaagt gagaagggaa ctttgatggc gacgttaccg gctaacgtcc ctggcgatat
    17041 cccggcgatt ggctttattt ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt
    17101 gaatccgcaa attgttgaaa actatcgcgg tggcgatatt gcgctgggta tcggcgatga
    17161 agttttatca ccggttatgt tcccggtgct gcatcagcta ctgggtcaga cgctgattac
    17221 caccgatggt aaaaccttgt taggtgccga tgacaaagca ggtattgcag aaatcatgac
    17281 cgcgctggcg gtattacaac aaaaaaacat tccgcatggt gatattcgcg tcgcctttac
    17341 cccggatgaa gaagtgggca aaggggcgaa acattttgat gttgatgcct tcgatgcccg
    17401 ctgggcttac accgttgatg gtggtggcgt aggtgaactg gagtttgaaa acttcaacgc
    17461 cgcgtcggtc aatatcaaaa ttgtcggtaa caatgttcac ccgggcacgg cgaaaggagt
    17521 gatggtaaat gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg cggatgaaag
    17581 cccggaaatg acagaaggct atgaaggttt ctatcacctg gcgagcatga aaggcaccgt
    17641 tgaacgggcc gatatgcact acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg
    17701 taaacgtaaa atgatggaga tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta
    17761 cattgaactg gtgattgaag acagttacta caatatgcgc gagaaagtgg ttgagcatcc
    17821 gcatattctc gatatcgccc agcaggcgat gcgtgactgc gatattgaac cggaactgaa
    17881 accgatccgc ggcggtaccg acggcgcgca gttgtcgttt atgggattac cgtgcccgaa
    17941 cctgttcact ggcggttaca actatcatgg taagcatgag tttgtgactc tggaaggtat
    18001 ggaaaaagcg gtgcaggtga tcgtccgtat tgccgagttg acggcgcaac ggaagtaagc
    18061 gaaaagggat gcggcatgtg atgccgcatc cggcttaaat ccaaacttac ccttcgaaga
    18121 accagtatcc gctattgacc agcgccgcga gcatcgcgag gaatgacgga tcttccagcg
    18181 catcgccaaa attctccgcg gtcagcgcaa tgttgctggc gagcgcatcc agtgccggac
    18241 ggtgcgggga atcgatcttc tcaccattgg catacacgtc gtcgccaatg cgcaatacgc
    18301 gcagaccccc caggcgcacc agcacatcac cttgtttcag cgcatcgtag atttcatccg
    18361 gctgataagg cggctctggc ggcgcgatat ccagttcatg acgtgactgg gatataaact
    18421 cgccaaacca ttgtttaaag tgttccggct ggttgatcaa ttcgagcatc atctcacgca
    18481 gtttatccat ctcttgcggc agaacgtccg caggatgagc gcgaggtgga acatcaggat
    18541 cgctgtagta gttgccgccc agttcacgtt gcagcacata atcggcaaat ccgctaatca
    18601 gttcccgcgt atttggcgcg cgaaaaccca cggaatagtt catcgcattt tccagcgcgt
    18661 agccttcatg cgggaatcct ggcggaatat aaagaatatc gccaggctcc agctcttcat
    18721 cgatgatggc ttcgaacgga tcgacctgta acaggtccgg gtgcgggcag tgctgtttca
    18781 tttgcagctt ttcgcccact cgccagcgac gacgaccggt accctgaatg ataaacacgt
    18841 cgtactgatc gagatgcggg ccgacgccgc cgcccggtac agaaaaagag atcatcagat
    18901 catcaatacg ccagtccggt agttcacgga acggtcgcat tagcgcagcg gtcggctcat
    18961 gccagtggtt cactgcttgc actaacaatg accagttagt ttcaccgaga tgatcgtagc
    19021 tttcgaatgg accgtggctg acctgccatt tgccatcctg atgactgacc agtcgactgt
    19081 cgacttcgct ttccatcgcc agacccgcca actcgtctgg agagatcggg tcaataaaat
    19141 tattaaagcc gcgttttaac accaccgggc gtttctgcca gtgacgttca agaaaatcgg
    19201 gccagttaag agtgagttgg tattccatgt taagcatcca caggctggta tctgcaaccg
    19261 attataacgg atgcttaacg taatgcgtga agtatggaca tatttattca tctttcggcg
    19321 cagaatgctg gcgaccaaaa atcacttcca tccgcgcacc gcccagcatg ctctctccgg
    19381 cgacgatttt accctcatat tgctcggtga tttcgcgggc taccgccagc cccacacctt
    19441 gcccagggcg taaagtatca acccgttgac cacggtcgaa aatgacctct cgcttgctta
    19501 atggaatacc ggggccatca tcctcgacca caatatagag atgctcgtcg gtttgccttg
    19561 cagaaatttc gacaaactcg aggcaatatt tacaggcgtt atccagcacg ttgcccatca
    19621 cttcgacaaa atcattctgc tcaccaacaa agctgatttc tggcgagata tcgagagaga
    19681 tattgacccc tttgcgttga tacactttgt tcagcgccga ggtgagattg tccaacagtg
    19741 gcgcgaccgg atgcagctcg cggctaagca atgtcccgcc gcgcatactg gcacgatgca
    19801 ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc cagcattacc ggctcagcat
    19861 cactgacgct catcttctca ctacgcagag aacgcagcgt actttgcagc accgccagtg
    19921 gcgttttcag actatgggtc aggtcggtga gcgttgtacg atatttgtcg taacgttcgc
    19981 gttcactttt taacagtcgg ttcaggtttc gtaccagact ggtcagttct cgcgttgtgg
    20041 ctggattgag caattcacgg ttatgttctt ccagttcgcg gacttctttt gccagggctt
    20101 cgatggggcg taaactccac caggcggcga cccacagcag cgggatcact aacagcagat
    20161 tggctgagag cacatagata aaccagctcc agaccatata ggaacttttt agctccaccg
    20221 gaatggtatc caccaccaca atggttaact ttggcatccg cgatgttgcc gggtagacgt
    20281 ttaccgccac cgagtgggtc atctccgcgt cgtcatcatc ttcccgcact tcctgcaact
    20341 gttgctgtat cgaatgatct ccactcagca agaggctggt atcgttaaca tccgcttcaa
    20401 tttcatgaaa accattcgat ttcagccagt caggctggat catcttcatc agccagggca
    20461 cgtcacgttg cgcccataaa agctgcccgt tctcatcata aattagtgtc atcgtggggc
    20521 tttgcttgtc gatattttcg ggtaactcga catgcaactt attgttttcc cacttcgcaa
    20581 gggtatagaa cagattgctc tcgccacgta acagccgaaa cgtggtttta tcgaaactga
    20641 cgctataacc gatcagcgca accattccgt aggcaagcga aagcaccaat actactgctg
    20701 ccgttgctaa cagaaaacgt acccgcagcg agagcgggaa aaaaagacgc agtaattttt
    20761 tcatcagcgc aattcgaaca gatagccctg gccgcgaacg gtggtaatca cttcttgcgg
    20821 atattgtgcc tgtatttttt tgcgcagacg tcccatcagt acatctatgg tatgactttc
    20881 ccgcagctcc gcatccggat agagttggag cattaacgaa tctttgctca ccactttgcc
    20941 attattgcgt atcaacgttt ccataatggt gtattcgaac gcggtcagtt tgatcacttc
    21001 gtcattaata gataattcac ggcgggagag atcaacctga aacgggggta gcgaaatgac
    21061 ctgtgaagcc agaccgctat tacgccgcat taatgcctgc attcgcgcca tcacctcttc
    21121 aatatgaaac ggtttagtca cgtaatcatc agcaccggca cttaatactt cgactttgtc
    21181 ctgccagctt tcacgggcgg ttaataccag aatcggcagt gaaacatcat tgctacgcca
    21241 gcggcgaatc agtgacagac cgtcctcgtc gggcaaaccg agatcgacaa tcgcaatatc
    21301 cggtaaatgt tcattgagat aataatcggc ttctttggca tcttctgcgt catcgacctg
    21361 atgaccagca tcctgaatct gaactttaag atggtgacgt aacaacgcat tgtcttcaac
    21421 aaccagtacg cgcatttttt atttctccct gtcttaatta ttaaaatagt gtaacgcgat
    21481 tatgtggtta tgggggtaaa cattaaataa accagcgggg aggggaggta aagtgaaaaa
    21541 ataaaaagtg gataatctta ataagcaggc cggacagcat cgccatccgg cactgttttg
    21601 aggcttattt cagctcatca accatggtga tggcgcgacc aatgtagtta gccggcgtca
    21661 tcgctttcag gcgggctttc tcttcttctg gcaacgccag accatcgata aactgcttca
    21721 tgccttcggc gtcaacgcgc ttaccgcgag tcagctcttt cagcttctcg tacggttttt
    21781 cgatgccata gcgacgcata actgtctgga ttggttcagc cagcacttcc cagttgtgat
    21841 ccagttcatc cagtagatgg tcacggttca cttccagttt actcacgcct ttcagggtgg
    21901 attgatacgc aatcagcgca taaccgatac ccacgccgag gttacgcagc acggtggagt
    21961 cggtcaggtc acgctgccag cgggaaactg gcagtttgct tgccaggtgc tgcaataccg
    22021 cgttggaaag ccccaggttt ccttcggagt tttcgaagtc gatcgggtta actttatgcg
    22081 gcatggtgga agaaccaatc tcaccagcaa tggttttctg tttgaagtgg ttaagggcga
    22141 tataacccca gacgtcacgg tcaaagtcga tcagaatggt gttgaagcgc gcaacgcaat
    22201 caaacagttc ggcaatgtag tcgtgcggtt cgatctgggt ggtgtacggg ttccactgaa
    22261 tacccagcga ggtgacgaac tcttcgctga actgatgcca gtcaacttcc gggtaagcgg
    22321 cgatgtgggc gttgtagtta ccgaccgcac cattgatttt gccgaggatc tccacctgat
    22381 taagctggcg gtactggcgc tccatacggt aggcgacgtt tgccatctct ttaccgatgg
    22441 ttgacggcgt ggctggctga ccgtgggtac gggacagcag cgggatatcg cgatactgaa
    22501 cggcgagatc tttaatgcca tcaatcagtt gacgccagta cggcaggatc acttcatcac
    22561 gcgcggtttt cagcattaat gcgtgggaga ggttattgat atcttccgaa gtacaggcaa
    22621 agtggatgaa ttcagaaacc gcgtgcagtt ccgggatctc cgccactttt tctttcagga
    22681 aatactcaac cgctttaacg tcgtggttag tggtacgctc gatggttttg atgcgtgctg
    22741 catcttcttc gctgaaactg gcgacaattg catcaaggta accgattgcg tcggcagcaa
    22801 aagcaggaac ttccttgatc gctgcgtgcg cggccagttt ttgcagccaa cgtacttcaa
    22861 cttgtacacg gaatttcagc aaaccgtatt cgctgaaaat cccgcgcagc gcgctgactt
    22921 tatcgccgta gcgtccatcg acaggggaaa cggcggtcag tgaggataat tccatagatc
    22981 acaactccgg ggttaaatga gcaagaattt gttttgcctg agtggtcagg cgattacgag
    23041 aaaacatcag ttgcagacga ccgccgccga cctggtgcca gagcacggcg gcgcgaatgc
    23101 ctgccagcag ggttgcgcga actttcgcct gcacctgtgg gctttgcagt acagcagggg
    23161 aaccagtgac ctgaatgcgc gggccaagcg ggctgataac atcaacatag atggcagcca
    23221 tcgcgctcat cagcgtttcg gactgtaaat cgaagtgttc gagctggcgt tgcaggccgt
    23281 tgatccggtt gcccagagtg tcgagcgcac ctttcgctga ggagagtttg cgctcaagca
    23341 ccatcagact gagtgtgtag cgggttaatt cggcgtttaa gccctggcga ctgctggcat
    23401 tgagtacgcc gagcaaggtt tccagcccga cgcgcaggtt ggcttcgcta ccgccaaaaa
    23461 ccgccagcgt tgagctgggg ttcatatcga taatactgtt gagtgagacg tgtagcgcat
    23521 cgccatcaca atgcccctgg tgagcgagtt gttgcaccag gcgtgccgac tgacaaatac
    23581 cggcaagggc gagggtgatg tcatagtaat tctttgccac gttcactgct tccttgtttt
    23641 aagtaaagat aataatcaga ccggcagcgg cagacgctgc tcaataatac cgccaccgag
    23701 gcacacttca ccgttataga aaacggcaga ctggcccggc gtcacggcgg caaccggctc
    23761 atcgaaaatc acttcaatgc gatcagcgtc cagcgccttg acggtgcacg ggatgtcggt
    23821 ctggcgatag cgggttttta ctgtgcaacg catagtacct gtgaacggct cgcgatcgac
    23881 ccagtgcagc tgctgggcaa tcagaccgac agacatcaga cgcgggtgtt catggccttg
    23941 cgcaacaatc agaatgttgt tttcgacgtc tttatcgacc acataccacg gttcttcggt
    24001 gccgtctttg gtgccaccga tacccagacc tttacgctga ccgagggtgt gatacatcag
    24061 cccctggtgc tcaccaattt catcgccatc gacggtaatg attttgcccg gttgcgccgg
    24121 gagataacgg cccaggaact cgcggaattt acgctcgccg ataaagcaga taccggtaga
    24181 atcttttttc ttcgcggtga ccagaccaag atcttcagca atcttacgca cctgcggttt
    24241 ttccagttcg ccgaccggga acaggctttg cgcaatttgc tcatggctga gcgtataaag
    24301 gaagtagctc tggtctttat tgctgtccag gccacgcagc aggcggctct tgccatcgac
    24361 atctgcccga cgtacgtaat gaccggtagc gatataatcg gcacctaaat cttcggcggc
    24421 aaattcgagg aaagctttaa atttgatctc tttgttgcac agaatatccg gatttggcgt
    24481 gcgaccggct ttatattcgg caaggaacag ttcgaagacg ttgtcccagt actcggcagc
    24541 aaagttaacg gtgtgcagtt caatgccgag cttgtcgcag acagcctggg catcagccag
    24601 atccgctgcc gctgtgcaat attcctcacc gtcgtcttct tcccagttct tcataaacag
    24661 gccttcgacc tgatatccct gttgttgcaa cagccaggca gaaacggagg aatcgacacc
    24721 gccggacatg ccgacgatta ctttttttgc ggtttcagac attggatcac tcacgacatt
    24781 gaacttcaag gcggcgtatt ctatcacgca gccttttaga tgacaccctt tgtaaaaggc
    24841 cagttaaaat cgccaatcat ctccagcgga tagcgttgcc cgctttgata acaacgaata
    24901 ctttccgcca ccagcggcga acgaagattt gacgcctgta aaatttcttc ggcgctgacc
    24961 caacggcagc agtcgatatc gctgtcatga ggctgcgtcg ggcatatttg ctcaagctca
    25021 atggcaaaga ggaaacgcaa aaacggcgtt ttatcaggcg caatccactg atgcatgcgg
    25081 ataaaatgtt gcggttgcgc gctgatgccg gtttcttccc acagctcacg ggcggcggct
    25141 tccactaagg tttcatctgc ttccagatgc ccggcgggct ggttccataa cgctttacca
    25201 ttaatcgtct cttcaacgac taaaaatttg ccttctgcgt gcaccacgca ggcaacggta
    25261 acgtgcggtt taaacatttt gcatccttaa tctgtcgcgt ctcgccattc gccattggca
    25321 agattatcca aagagtaatt ccccatcgca tagcgaatca gtcgcagcgt ggggaaacca
    25381 acatgggcgg tcatgcggcg cacctggcga ttacgtcctt catataaggt gatcttcagc
    25441 cagctggtgg gaatactttt gcgctcacga attggtggat tccgcggcca taaccacgcg
    25501 ggttcttcaa ccagctccgc gcctgcgggc agggtagggc catcatttaa ggttacacca
    25561 ttgcgcaagg cttcaagggc gtcttgtgtg ggaatacctt ctacctgtac ataatagatt
    25621 ttcccggtac gtttacccgg ctgggttaaa cgcgcctgca aagcgccgtt attggtcagc
    25681 accagtaacc cttcgctgtc gcggtcgaga cgacctgccg catacacccc ctgaaccggg
    25741 atgaattctt ttaatgtttt gcgtccggct tcatcggtga actgcggaag aacatcgtag
    25801 ggtttattga acaggatcac acgcgtgggc tggttgtcag gtttacgcct ggtagaacgt
    25861 tgcgagctga atcgcttaac ctggtgattt ctaaaagaag ttttttgcat ggtattttca
    25921 gagattatga attgccgcat tatagcctaa taacgcgcat ctttcatgac ggcaaacaat
    25981 agggtagtat tgacaagcca attacaaatc attaacaaaa aattgctcta aagcatccgt
    26041 atcgcaggac gcaaacgcat atgcaacgtg gtggtagacg agcaaaccag tagcgctcga
    26101 aggagaggtg aatggaaagt aaagtagttg ttccggcaca aggcaagaag atcaccctgc
    26161 aaaacggcaa actcaacgtt cctgaaaatc cgattatccc ttacattgaa ggtgatggaa
    26221 tcggtgtaga tgtaacccca gccatgctga aagtggtcga cgctgcagtc gagaaagcct
    26281 ataaaggcga gcgtaaaatc tcctggatgg aaatttacac cggtgaaaaa tccacacagg
    26341 tttatggtca ggacgtctgg ttgcctgctg aaacccttga tctgattcgt gaatatcgcg
    26401 ttgccattaa aggcccgctg accactccgg ttggtggcgg tattcgctct ctgaacgttg
    26461 ccctgcgcca ggaactggat ctctacatct gcctgcgtcc ggtacgttac tatcagggca
    26521 ctccaagccc ggttaaacac cctgaactga ccgatatggt tatcttccgt gaaaactcgg
    26581 aagacattta tgcgggtatc gaatggaaag ctgactctgc cgacgccgag aaagtgatta
    26641 aattcctgcg tgaagagatg ggcgtgaaga aaattcgctt cccggaacat tgcggcatcg
    26701 gtattaagcc gtgttctgaa gaaggcacca aacgtctggt tcgtgcagcg atcgaatacg
    26761 caattgctaa cgatcgtgac tctgtgaccc tggtgcacaa aggcaacatc atgaagttca
    26821 ccgaaggcgc gtttaaagac tggggctacc agctggcgcg tgaagaattt ggcggtgaac
    26881 tgatcgacgg cggcccgtgg ctgaaagtta aaaacccgaa taccggcaaa gagatcgtca
    26941 ttaaagacgt gattgctgat gcattcctgc aacagatcct gctgcgtccg gctgaatatg
    27001 atgttatcgc ctgtatgaac ctgaacggtg actacatttc tgacgccctg gcagcgcagg
    27061 ttggtggtat cggtatcgcc cctggcgcaa acatcggtga cgaatgcgcc ctgtttgaag
    27121 ccacccacgg tactgcgccg aaatatgccg gacaggacaa agtaaaccca ggctctatta
    27181 ttctctccgc tgagatgatg ctgcgccata tgggctggac tgaagccgca gacctgattg
    27241 ttaaaggtat ggaaggcgca atcaacgcga agaccgtaac ctatgacttc gaacgtctga
    27301 tggaaggcgc taaactgctg aaatgttcag agtttggtga cgcgatcatc aagaacatgt
    27361 aatctctaca tgtgttaaat attgaaacgg gcgtataaca cgcccgttgt tttatgatga
    27421 tgtaaaatct tccccaaaac tttccccaaa acccttcccc aaaactggct attttctatg
    27481 ctgttttgat atctacgata atccagtctt taccacgatc atcattgtat cggtcggtca
    27541 tttttctgga tttatggcct aacaactttt gcgtatccag accctgttcc cgatataacc
    27601 gttctgacag agatcgctgc tcatgaaatg tgggcgcagt tccttgctcc cattttatgc
    27661 cacatttttc cctggccttt ttaaaagccg ttgtcagagt attcgcagaa acctggtctc
    27721 ctctgtttgc ttgagaggtt gtgtgacggt aatggaccag atatttacta acaacagcat
    27781 ccctgcactg agatataact tcacgaaggg taatattcag agcatcgcat ttcaggctaa
    27841 gcggaatagc aagttttgaa ccggtttttt cctgagtaat gtgcaacatg tcgtcccata
    27901 tatcagagaa tttcaaattg cagatatcgc ctaaacgttg tccagtaaca agagcaagta
    27961 gcatgccgca ttttaaatag ggctgccgtc tgcttacgct ttcaaatatt gcctgccatt
    28021 cgggcagtga caatctttgg cggtttactc gatttcgcgg ttgttttgtt gcctgcgctg
    28081 ggttaaatcc tggcggaaca tgtcctgcgt gttgtgcttc tttgaagacg tcgatcaaca
    28141 ccattctcac gacttgcgcc atcctgttat gaccttcagc ctttacagca tcaattattt
    28201 cggcaatatc aagtgcggta atatccttga ggtgttgcat tccacaatgc tcacggaaaa
    28261 gacgaatggg tttgcctttt tgccgatagg agttgggtct tagttcatta tgttgcagcc
    28321 tgtcctcctg gatagaaata tatttatcaa gccattctgt caccgtaatg tctgagcgcc
    28381 tgcctttcat tctttccaga cgctcattga cgcttaatat ttgtcgggta cgttgttcag
    28441 caataatggt atttgcttca gtagcaactt gttttgcttc attctcatca gttcctaagc
    28501 tatgaaaacg accggatagt ggatgtttgt attgccaata tacctttccg gttcgcttat
    28561 ctaatttgca atataaattg ggtatagaga ttttgtgaga tcggggtcta gcagccatca
    28621 gcgattatcc gttggagttt tgggtttgcg tttattggga gttgcggttc tgcaagcgtt
    28681 cctacaaaac gggaatttcg gtcaatcatc cagtagcgac caacttttat agcgggtggg
    28741 gccatcattt tcccttgcgc gtattttttc agaactcgct cacttggtgc taagtcccca
    28801 aattcttctt tagcccagtc ctgtaaagtg attagtcgag acatttgtcc tcctcttagc
    28861 tgctgaggga gtttgtgacc gatatatctg acatgatatt aagctcatgg caggtacatc
    28921 tcttgactgg tcatagagat aaatttaatg ctgagaaatg cagtattgaa tttatcaatt
    28981 tttctatttc ctgcgtatgg cacgtaactt cttaatgtgt tctgctgtat cgatctcttc
    29041 ggctatccgc tctgattcca ctttactcac aggttcaaag tcatgattaa agcggaacat
    29101 gctggcgata catgttctgc cttttcggat gtagtgaact ttgttgtggg tagaacgcag
    29161 gattttgcag ggagtgccgt ggtggtcgac gtaccaggtg ttagggtaaa tcactctgaa
    29221 cattcttgac cttgtgttgt gcggattgat attcagtgct gatattcacc tttatagcga
    29281 acacctttac cggtttatcg ccaaagtgcg gatgtgtgat tgtcttcact tcatatccgt
    29341 catacggaac atcaattctg cgactggaat cgtcgcgctt cggatatccc tttgtgataa
    29401 tcaggcggtc atattcccgg aacataattc gcttattcca gtagtcatta cacaagcgat
    29461 actcttctgt tttctctccg cgaatcatgg catcgaagta ttcactttta acagcaagtt
    29521 gcaggttagc catcacttaa tccccctttg tttgcggata agctccagat cttgctggca
    29581 actggcgcaa gtacgacaac cctgaacggc cagtcgtctt cgttcatcta tcggatcgcc
    29641 acactcacaa caatgagtgg cagatacagc taggtagttc aggcggcgca tttttattgc
    29701 tgtgttgcgc tgtaattctt ctatttctga tgctgaatca atgatgtctg ccatctttca
    29761 ttaatccctg aactgttggt taatacgctt gagggcgaat gcgaataata aaaaaggagc
    29821 ctgtagctcc gtgatgattt tgtttttcat gttcaccgtt ccttaaagac gccgtttaac
    29881 atgccgatcg ccaggcttaa atgagtcggt gtgaatccca tcagcgttac cgtttcgcgg
    29941 tgcttcttca gtatgctacg gcaaatgtca tcgacgtttt tatccggaaa ctgctgtctg
    30001 gcttttttga tttcatagtt agcctgacgg gcaatactgc gaagggcgtt ttcttgctga
    30061 ggtgtcattg aacaagtccc atgtcggcaa gcataagcac acagaatatg aagcccgctg
    30121 ccagaaaaat gcattcagtg gttgtcatac ctggtctctc tcatctgctt ctgctttcgc
    30181 caccatcatt tccagctttt gtgaaaggga tgtggctaac gtatgaaatt cttcgtctgt
    30241 ttctactggt attggcacaa acctgactcc aatttgagcg aggctatgtg ccatctcgat
    30301 actcgttctt aactcaacgg gagatgcttt gtgcatacag cccctcgttt attatttatc
    30361 tcctcagcca gccgctgggc tttcagtgga ttttggataa cagaaaggcc gggaaatacc
    30421 cagcctcgct ttgtaacgga gtagacgaaa gtgatcgcgc ctacccggat attatcgtga
    30481 ggatgcgtca tcgccattgc tccccaaata caaaaccaat ttcagccagt gcctcgtcca
    30541 ttttttcgat gaactccggc accatctcgt caaaactcgc catgtacttt tcatcccgct
    30601 caaccacgac ataatgcagt ccttcacgct tcatacgcgg gtcatagttg gcaaagtacc
    30661 aggcatcttt tcgcgtcacc cacatgctgt actgcacctg ggccatgtaa gccgatttta
    30721 tggcctcgaa accaccgagc cggaacttca tgaaatcccg ggaggtaaac gggcatttca
    30781 gctcaaggcc gttgccgtca ctgcataaac catcgggaga gcaggcggtg cgcatacttt
    30841 cgtcgcgata gatgatcggg gattcagtaa cattcacgcc ggaagtaaac tcaaacaggg
    30901 ctctggcgtc gttctcgtac tgttttcccc aggccagcgc cttagcgtta acttccggag
    30961 ccacaccggt gcaaacctca gcaagcagag tgtggaagta ggacattttc atgtcaggcc
    31021 atttctttcc ggagcggggt tttgctatca cgttgtgaac ttctgaagct gtgatgacgc
    31081 cgagccgtaa tttgtgccac gcatcatccc cctgctcgac agctctcacg tcgatcccgg
    31141 tacgctgcag gataatgtct ggtgtcatgc tgccaccttc tgctcagtgg ctttttgttt
    31201 caggaatcca agagctttca ctgcttcggc ctgtgtcagt tctgacgatg cacgaatgtc
    31261 gcggcgaaat atctgggaac agagcggcaa taagtcgtca tcccatgttt tatccagggc
    31321 gatcagcaga gtgttaatct cctgcatggt ttcatcgtta accggagtga tgtcgcgttc
    31381 cggctgacgt tctgcagtgt atgcggtatt ttcgacaatg cgctcggctt catccttgtc
    31441 atagatacca gcaaatccga aggccagacg ggcacactga atcatggctt tatgacgtaa
    31501 catccgtttg ggatgcgact gccacggtcc ggtgatttct ctgccttcgc gggttttgaa
    31561 tggttcgcgg cgacattcat ccatccactc ggtaacgcag atcggatgat tacggtcctt
    31621 gcggtaaata cggcatgtac atgattcatt gtcctgctca aagtccatgc catcaaactg
    31681 ctggttttca ttgatgatgc gggaccagcc atcaacgccc accaccggaa cgatgccatt
    31741 ctgcttatca ggaaaggcgt aaatttcttt cgtccacgga ttaaggccgt actggttggc
    31801 aacgatcagt aatgcgatga actgcgcatc gctggcatca cctttaaatg ccgtctggcg
    31861 aagagtggtg atcagttcct gtgggtcgac agaatccatg ccgacacgtt cagccagctt
    31921 cccagccagc gttgcgagtg ctgtactcat ccgttttata cctctgaatc aatatcaacc
    31981 tggtggtgag caatggtttc aaccatgtac cggatgtgtt ctgccatgcg ctcctgaaac
    32041 tcaacatcat catcaaacgc acgggtaatg gcttttttgc tggccccgtg acgttgcaaa
    32101 tgatcgatgc agagtgattc aaacaggtgc tggggcagac ctttttccat gtcgtctgcc
    32161 agttctgcct ctttctcttc acgggcgatc tgctggtagt gacgcgccca gctctgagcc
    32221 tcaagacgat cctgaatgta ataagcgttc atggctgaac tcctgaaaat ggctgtgaaa
    32281 atatcgcccg cgaaatgcca ggctgattag gaaaacagga aaggggatta gtaattcagg
    32341 ccgttaccgc gtccgtcgag aaaaacttcc acgagcaaat cacgggtata agtgcgctcg
    32401 atgccgcgat gcagatatag ccgtccgcgt aaattagctg atgcagtcca ggtagcatct
    32461 ttgtgtttga ccagcattcc tggcatgacc gcaccgcgat taacggtctg tgttccgtaa
    32521 tgttgatgaa tcataaaaac tcctgcccgt aagctgggct gctgaacata tagagacttc
    32581 tgcgcgtatt caggcggtgg atggccgccg gttgtcataa ctaagtcgcc tcgttgaagc
    32641 gactgaggta tgaagtgttg agttgatttc agctggtcac accgacgttc acgcgtccgc
    32701 ttcacccctc gcactccccg gagcctgctg aaattcacgc tgcggatcta agcggtcatc
    32761 gcaacggtga atcaggtgat tgccgtatcg ttgtgttgtt gcgatgaatt tatttaaaac
    32821 tatagttgtt ttatcgtcaa caacaaaagt tgttttattg gttgttttag atataactgg
    32881 ttgtattttg gatggattta ttttgtgact tgcatcgcat agcgataact gaagcgaggt
    32941 cgtggtggtt ttttgaacgg tttgtgtgat gaggggaggc aaaagaaaac ccggcaaggc
    33001 cgggttactt atgttttgac agattaagta attgcatcag gagcatgcca atatggatta
    33061 gtgacgtacc tgataaacca atagcgattg atggtagcat tagtatagga tttttacttg
    33121 ttgcaagtaa aaacaagcca ttaaagaaaa gtactcctag ctcgcaaaaa gttaatccat
    33181 acatgattat gattgatgtc atatagccat atgttttcaa tttgcgaata ttcctgcctc
    33241 ccagccccat cagaaatgtt aaagcggcta taagtatcgc aattgtagta ccagaataag
    33301 cggcaatagc gccacccagc gcatctctgt gctcgctaaa agccataccc ggaaacatgt
    33361 ggcggaatag ataatacgca cttataataa ttgggaatgg caaaagaaaa agtattattg
    33421 ctggcgctat cctgtgccgt gacttcatat agccccccaa tgttatagtg gtaactcatc
    33481 tccaaatgtt ctattataac tttccattat aatcaatttc atcctgagat agcaagttga
    33541 catttcggat gctatgtctt cattcgatga tttatagata tttgctccaa tgtggccttt
    33601 gtcagaaaga taatattctg taagaatatc tgcagcttgt tctttaccct tcaggtgaat
    33661 gctctcatgt tgttcatcca cattgctaat aatccctttg gcaacagatt ttatatttcg
    33721 taaacgttta ggcttcacgg ttatttccag tccttctaag agctcttcgt caactgcttg
    33781 tgcaccaagc actctaagta gggggctgag aagttttgac ccattttcaa cccttaccgt
    33841 tgttcttcct ataaactgca tgcttaaagc atcatccttt gttatatctc tcatcaaagg
    33901 ttctatgaat agtgagtatc catcaggtat tgctcccttt ccttttaggt aagccgcaag
    33961 atcacgagtt tttgggccga ataatgtatt tgcatatccg ataatatttt ttcttattaa
    34021 taaaaaggat gggaatccta atgactcatc tgaactaagc gcatttctta tttcatcaac
    34081 cgaaagtgtt tttctattga tttttttaac tagttctgaa tcaaatgttt ttgtcaggat
    34141 aaaactatca ttaattatat gatgtgcaaa aacatagtag tcttcaatct gctgagcatg
    34201 attcttagcg tttattacct gctctgtcag aaatgacaat aaatctatag gttgtttttt
    34261 cagagagcta gtattttgaa tgtatacggc ataaaagtta agcttcattg ctattccatc
    34321 tatgtatcaa agaaaaaatg ttgatatgta tagtcttttt acgtagttat agtattcgat
    34381 tcggtagtta cccatgcttc ctgtatgtct gcggcatgct cccaataacc ttaccgaaga
    34441 tgaatacccg gttcatctcg tctttctcaa ttgggtccca cggcgagtag ctcttgttat
    34501 cagagataac cagtagctta tccttcatca tttgcaggcg ctttacatgg gctgtgtcgt
    34561 cgtacagaaa cgcatagata ccatcaccgt cgaaagattt aaccgtgata tcaacgaaca
    34621 gaagatcacc tggttcgatc gttcctgaca tgctgtcacc gcgtacgtta atgatgcgga
    34681 tattttctgc cttcctacca tcgaacatgt gacgagcatc gtcaaacgag tactcaaccg
    34741 agcgtagaac ttctacaaac tcacggttga tgactcccgg cccggcactc acttctatat
    34801 caagaacgtc aatcttaaag tatttggaat ggctgacagt tgattgtatt ggttgcactg
    34861 tactgtctga catatttcca acgccagaag ataaccattc tgcgcgcaca cccaaagcgt
    34921 tcgcgatctc cacgatttta gttgtttggt tagctttccc tgtttcgatt ttctgaatag
    34981 cagcttggct aaccccgacc aaatccccaa gcgccttttg tgtaaggcct cgcgctaatc
    35041 tggcttcttt aagtctttct gagagtgttg ttttcatagt ccaaatgtac aaccaaggtt
    35101 ttatttcatc aagcgaaaat ggttgttgac taaaaacaac catagtttta atcttgattc
    35161 aaattaacca cggaggttgt tatgaaccca gctatcaaaa cagcgatcaa tatcgttggt
    35221 tcacaaaaga aactgggcgc tgcctgcgaa gtttcacagc aggccgtcta taagtggctt
    35281 cacaacaaag caaaggtatc ccctgaacat gtcggcagca ttgttacggc tactggtgga
    35341 gtagtgaagg cataccagat tcgcccggat cttccgaagt tgtttccaca caccgaaaag
    35401 aacgcagctt aaatttccat ttcacgctct ttaacaataa gcaatcaact taacagtcaa
    35461 ttcaaactaa aggagtcaat tatgcaacca cttacatacc aacagactag cggatttagc
    35521 ccgactgcgg tgataaatcg ttctcaaaca aaacaggtgc caggccacga aaaaatccgt
    35581 gatgccgtcc gcgcctggtc ggctgcagat aatcaggatg ttgttgccgc actcattgtg
    35641 aatgagtatc gggagcaggg cggcggcacc atcgatttcc ctgatgatgt cagtcgtgca
    35701 cgccagaagc tgttccgctt cctcgataac aaattcgatt ctgaaaaata ccgaaataac
    35761 gtgcgtgaac tgaccccggc aattctggcg gtactaccgc tggaatatcg cggttacctg
    35821 gttgagcagg atagcttcat ggctaggttg gctgaaatgg aaaaggagct cagtgaggca
    35881 aaacaggctg tcattctcaa cgcaccacgc caccagaaac tgaaggaaat gagtgaaggt
    35941 attgtgtcga tgtttcgtgt ggacccggat ctggctggtc cactgatggc gatggtcacc
    36001 accatgctgg gggcaatatg acaggttcgg aaatggcgaa agccggtctg cgcgaacaga
    36061 accgactttc aggtgcaaat cgtaacacac tcattgcggg aggaattatg gcaaacactg
    36121 ctgagatatt caattttcca gtgccggatg tggcacaaaa ggagccgcgc gtggcagatc
    36181 tcgatgatgg ttatacgcgc attgcaaatg agttgctgga agctgtgatg ctggccggat
    36241 taacacagca ccagcttctg gtcttcctgg ctgtcatgcg caaaacatat ggctttaata
    36301 aaaaactgga ttgggtgagc aacgagcaac tttccgagtt gaccgggata ttgccgcaca
    36361 agtgttctgc tgcaaaaagt gttctggtaa agcgtgggat ttttattcag agcgggcgga
    36421 atatcggcat taataatgtg gtcagtgaat ggtcaacatt acccgaatca ggtaagaaaa
    36481 ataaagttta cctgaaagag gtaaatttac ctgaatcagg taagaaaagt ttacccaaat
    36541 caggtaaaga cgtttacccg aatcaggtaa acacaaaaga caaactaaca aaagacaata
    36601 taaaaccttt ttcgtccgag aattctggcg aatcctctga ccagtcagaa aacgatcttc
    36661 ctgtggtgaa accggatgct gcaattcaga gcggcagcaa gtgggggaca gcagaagacc
    36721 tgaccgccgc agagtggatg tttgacatgg tgaagaccat cgcgccatca gccagaaaac
    36781 cgaattttgc agggtgggct aacgatatcc gcctgatgcg tgaacgtgac ggacgtaacc
    36841 accgcgacat gtgcgtgctg ttccgctggg catgccagga caacttctgg tccggtaacg
    36901 tgctaagtcc ggccaaactc cgcgacaagt ggacccaact cgaaatcaac cgtaacaagc
    36961 aacaggctgg agtgacagcc ggaaaaccaa aactcgacct gacaaacact gactggattt
    37021 acggggtgga gctatgaaaa atattgccgc gcagatgatt aactttgacc gtgagcagat
    37081 gcgtcgaatc gccaacaaca tgccggaaca atacgacgaa aagccgcagg tgcagcaggt
    37141 ggcgcagatc atcaacggag tgttcagcca gctgcttgcg acatttccgg caagcctcgc
    37201 gaaccgcgac cagaacgagc tgaacgaaat ccgccgtcag tgggttatgg ctttccgtga
    37261 aaatggaatt accactatgg agcaggttaa cgcaggaatg cgcgtagccc gtcggcagaa
    37321 tcgaccattc ctgccatcac ccgggcagtt tgtggcctgg tgccgggaag aagcatccgt
    37381 taacgccggg ctgccaaacg tcagcgagct ggttgatatg gtttacgagt attgccggaa
    37441 gcgtggcctg tatccggatg cagagtctta tccgtggaaa tcgaacgcgc attactggtt
    37501 ggttaccaac ttgtaccaga acatgcgggc caatgcgctg actgacgcgg aattacggcg
    37561 caaggctgcc gatgaactga cttgtatgac agcgcgaatt aaccgtggtg aggctatacc
    37621 tgaaccagta aaacaaattc ctgtcatggg cggtagacct ctaaatcgtg cacaggctct
    37681 ggcgaagatc gcagaaatca aagctaagtt tgggctgaaa ggagcaaggg tatgacgggc
    37741 aaagaggcaa ttattcatta cctggggact cataagaact tctgtgcgca ggacgttgcc
    37801 gcggtaacag gcgcaaccgt aaccagcata aatcaggctg cggctaaaat ggcgcgggca
    37861 ggaatcctgg tcgttgatgg taaggtctgg cgaacggtgt attatcggtt cgctaccaga
    37921 gaagaacggg aaggaaagat gagcacgaac ctgattttta aggagtgtcg ccagagtgcc
    37981 gcgatgaagc gagtgttggc gatgtatggg cgaggatgag tgatggaacg gggcttgacc
    38041 tgtatgctgt ccgtgtactt gcaaggaaat ttagatgcag tacgagatga gtccatcaaa
    38101 aataaactca aaaatcaaag tgagtaggta catcgtttgc aatcgacaat gccctataat
    38161 agatgttgaa taattgcata aaatgatgct atacctcttt tgccttattc atattgagtt
    38221 taattcttct caacagaaag gacttatcga cgcacgataa gtgtcatata tgcaattgtt
    38281 tatcaacaca aaatctgagg aattatgatt actagaaatc ttggcgaaga aacaatcaat
    38341 agtattatcg aagaagataa tctgctgtca gagcaggttc aagtcattga tggttgcatc
    38401 gttataaata ttgaatatga gtaccccatt cccttgaata catgctcaac ttatcaacaa
    38461 attttgcatt gggtatggca cttggcggaa aaaacatgga tgacgcaaat tgtcactcgt
    38521 cgtttcatcg aaattgcc
//
LOCUS       DAZKHK010000033        37954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKHK010000033 DAZKHK010000000
VERSION     DAZKHK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.033"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(123..410)
                     /gene="yebO"
                     /locus_tag="ACHOS5_001728"
     CDS             complement(123..410)
                     /gene="yebO"
                     /locus_tag="ACHOS5_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP9776550.1"
                     /translation="MNEVVNSGVMNIASLVVSVGVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(485..628)
                     /gene="mgrB"
                     /locus_tag="ACHOS5_001729"
     CDS             complement(485..628)
                     /gene="mgrB"
                     /locus_tag="ACHOS5_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP9776551.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            787..1026
                     /gene="yobH"
                     /locus_tag="ACHOS5_001730"
     CDS             787..1026
                     /gene="yobH"
                     /locus_tag="ACHOS5_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP9776552.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1170..1961)
                     /gene="kdgR"
                     /locus_tag="ACHOS5_001731"
     CDS             complement(1170..1961)
                     /gene="kdgR"
                     /locus_tag="ACHOS5_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP9776553.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLSVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2138..3511
                     /gene="yebQ"
                     /locus_tag="ACHOS5_001732"
     CDS             2138..3511
                     /gene="yebQ"
                     /locus_tag="ACHOS5_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776554.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3557..4438)
                     /gene="htpX"
                     /locus_tag="ACHOS5_001733"
     CDS             complement(3557..4438)
                     /gene="htpX"
                     /locus_tag="ACHOS5_001733"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP9776555.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4630..6678)
                     /gene="prc"
                     /locus_tag="ACHOS5_001734"
     CDS             complement(4630..6678)
                     /gene="prc"
                     /locus_tag="ACHOS5_001734"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP9776556.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLESDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6698..7396)
                     /gene="proQ"
                     /locus_tag="ACHOS5_001735"
     CDS             complement(6698..7396)
                     /gene="proQ"
                     /locus_tag="ACHOS5_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP9776557.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7493..7990)
                     /gene="msrC"
                     /locus_tag="ACHOS5_001736"
     CDS             complement(7493..7990)
                     /gene="msrC"
                     /locus_tag="ACHOS5_001736"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP9776558.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     TSNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8120..9403
                     /gene="yebS"
                     /locus_tag="ACHOS5_001737"
     CDS             8120..9403
                     /gene="yebS"
                     /locus_tag="ACHOS5_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP9776559.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPASLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9372..12005
                     /gene="yebT"
                     /locus_tag="ACHOS5_001738"
     CDS             9372..12005
                     /gene="yebT"
                     /locus_tag="ACHOS5_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP9776560.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGSVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12085..13524
                     /gene="rsmF"
                     /locus_tag="ACHOS5_001739"
     CDS             12085..13524
                     /gene="rsmF"
                     /locus_tag="ACHOS5_001739"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP9776561.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNKGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGD
                     LFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13642..13878
                     /gene="yebV"
                     /locus_tag="ACHOS5_001740"
     CDS             13642..13878
                     /gene="yebV"
                     /locus_tag="ACHOS5_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP9776562.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13983..14174
                     /gene="yebW"
                     /locus_tag="ACHOS5_001741"
     CDS             13983..14174
                     /gene="yebW"
                     /locus_tag="ACHOS5_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP9776563.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14175..14831)
                     /gene="pphA"
                     /locus_tag="ACHOS5_001742"
     CDS             complement(14175..14831)
                     /gene="pphA"
                     /locus_tag="ACHOS5_001742"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9776564.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLINGGD
                     WFIALADNQQKQAKTALEKCQHLPFILELHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15226..15567)
                     /gene="yebY"
                     /locus_tag="ACHOS5_001743"
     CDS             complement(15226..15567)
                     /gene="yebY"
                     /locus_tag="ACHOS5_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP9776565.1"
                     /translation="MMRKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15580..16452)
                     /gene="copD"
                     /locus_tag="ACHOS5_001744"
     CDS             complement(15580..16452)
                     /gene="copD"
                     /locus_tag="ACHOS5_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP9776566.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16456..16830)
                     /gene="yobA"
                     /locus_tag="ACHOS5_001745"
     CDS             complement(16456..16830)
                     /gene="yobA"
                     /locus_tag="ACHOS5_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP9776567.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16969..17199
                     /gene="holE"
                     /locus_tag="ACHOS5_001746"
     CDS             16969..17199
                     /gene="holE"
                     /locus_tag="ACHOS5_001746"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP9776568.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17301..17957
                     /gene="yobB"
                     /locus_tag="ACHOS5_001747"
     CDS             17301..17957
                     /gene="yobB"
                     /locus_tag="ACHOS5_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP9776569.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17980..18642
                     /gene="exoX"
                     /locus_tag="ACHOS5_001748"
     CDS             17980..18642
                     /gene="exoX"
                     /locus_tag="ACHOS5_001748"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP9776570.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18639..20699)
                     /gene="ptrB"
                     /locus_tag="ACHOS5_001749"
     CDS             complement(18639..20699)
                     /gene="ptrB"
                     /locus_tag="ACHOS5_001749"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP9776571.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     QSAD"
     gene            complement(20908..21567)
                     /gene="yebE"
                     /locus_tag="ACHOS5_001750"
     CDS             complement(20908..21567)
                     /gene="yebE"
                     /locus_tag="ACHOS5_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9776572.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21894..22250)
                     /gene="yebF"
                     /locus_tag="ACHOS5_001751"
     CDS             complement(21894..22250)
                     /gene="yebF"
                     /locus_tag="ACHOS5_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP9776573.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22317..22607)
                     /gene="yebG"
                     /locus_tag="ACHOS5_001752"
     CDS             complement(22317..22607)
                     /gene="yebG"
                     /locus_tag="ACHOS5_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP9776574.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22741..23919
                     /gene="purT"
                     /locus_tag="ACHOS5_001753"
     CDS             22741..23919
                     /gene="purT"
                     /locus_tag="ACHOS5_001753"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP9776575.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23975..24616)
                     /gene="kdgA"
                     /locus_tag="ACHOS5_001754"
                     /gene_synonym="eda"
     CDS             complement(23975..24616)
                     /gene="kdgA"
                     /locus_tag="ACHOS5_001754"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9776576.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24653..26464)
                     /gene="edd"
                     /locus_tag="ACHOS5_001755"
     CDS             complement(24653..26464)
                     /gene="edd"
                     /locus_tag="ACHOS5_001755"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP9776577.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26699..28174)
                     /gene="zwf"
                     /locus_tag="ACHOS5_001756"
     CDS             complement(26699..28174)
                     /gene="zwf"
                     /locus_tag="ACHOS5_001756"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9776578.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28512..29381
                     /gene="hexR"
                     /locus_tag="ACHOS5_001757"
                     /gene_synonym="yebK"
     CDS             28512..29381
                     /gene="hexR"
                     /locus_tag="ACHOS5_001757"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP9776579.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29509..30951
                     /gene="pyk"
                     /locus_tag="ACHOS5_001758"
     CDS             29509..30951
                     /gene="pyk"
                     /locus_tag="ACHOS5_001758"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9776580.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31083..32054)
                     /gene="lpxM"
                     /locus_tag="ACHOS5_001759"
                     /gene_synonym="msbB"
     CDS             complement(31083..32054)
                     /gene="lpxM"
                     /locus_tag="ACHOS5_001759"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9776581.1"
                     /translation="MDTKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32173..33495)
                     /gene="mepM"
                     /locus_tag="ACHOS5_001760"
     CDS             complement(32173..33495)
                     /gene="mepM"
                     /locus_tag="ACHOS5_001760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP9776582.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33511..34443)
                     /gene="znuA"
                     /locus_tag="ACHOS5_001761"
     CDS             complement(33511..34443)
                     /gene="znuA"
                     /locus_tag="ACHOS5_001761"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP9776583.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            34522..35277
                     /gene="znuC"
                     /locus_tag="ACHOS5_001762"
     CDS             34522..35277
                     /gene="znuC"
                     /locus_tag="ACHOS5_001762"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP9776584.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLSRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35274..36059
                     /gene="znuB"
                     /locus_tag="ACHOS5_001763"
     CDS             35274..36059
                     /gene="znuB"
                     /locus_tag="ACHOS5_001763"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP9776585.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36204..37214)
                     /gene="ruvB"
                     /locus_tag="ACHOS5_001764"
     CDS             complement(36204..37214)
                     /gene="ruvB"
                     /locus_tag="ACHOS5_001764"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9776586.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37223..37834)
                     /gene="ruvA"
                     /locus_tag="ACHOS5_001765"
     CDS             complement(37223..37834)
                     /gene="ruvA"
                     /locus_tag="ACHOS5_001765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9776587.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9114 a         9790 c         9327 g         9723 t
ORIGIN      
        1 atgataatta tttgaagtca gaccagagtt ttaatttgaa gcagctgcac catgacagtg
       61 cgtataatca gcatgaattg agtaaccctt caacgacggg gttactcaag attgaggaag
      121 gattatcgtt cggcgaccag acgaataata tcttcgtctt caaccgattt ttgactctca
      181 acggtctttt tatctgcttt ctcaggttcg tttgcttcgc acaaacgacg taacagtgca
      241 ttttgacgtt tttgctgatc cagcaacgcc tcaagcagtt caatctgttc gttagtccgt
      301 gaactggcac gattgataaa aaaccacaag atgagcccga taagaagaac ccccaccgat
      361 acaaccaaag acgcaatgtt catcacgcct gaatttacaa cttcgttcat ttcaccacct
      421 ctgagtagag gcgctattct accactgctg gagaggaaga aaatctagtg ctgaaaaaat
      481 gatatcacca cgggataaac tggttaatgg cacaaattcc gctgaaaaat tgtacatcct
      541 gatcgcacat catgttgaat acctgcgccc aaagcagcaa gcaagccaac accacgacaa
      601 ccagagcgac ccatcgaaac tttttcactc caatctccgt ttcacctacc ttatgtcatt
      661 aaactagcat gcgttagtta aacagcgcct aactatgtcg atattcgtgc tttttcggaa
      721 tgagtcgctt gcttcagcgc atattgccgc tacgattaag cgaacataaa aagagaagag
      781 gttgtaatgc gattcatcat tcgcacagtt atgctgatag cgctggtatg gataggatta
      841 ttactcagcg gatatggcgt tttgattgga agcaaagaga atgccgcggg attaggttta
      901 caatgtacct atctgaccgc cagaggcacc agcacggtac aatacctgca cactaaaagt
      961 ggttttctgg ggataacaga ttgcccgttg ttacgtaaaa gcaatattgt ggtcgataat
     1021 ggttaaggat aataaataac cagattctca ctcatgagta aaatattctt atcgcaagtg
     1081 cgtttaatat aacgccacct tcaactttat ttttctcatg ctaaataaag aacctgtctg
     1141 tttgatattc agacaggttc ttactctcat cagaacggat agtcgtgata acccatttgg
     1201 gcagaaattt tgcgcgccgc ggtatgcaac attgcgacat attcctgtaa acgctcttca
     1261 gagaaacgta acgtcgggaa ggagatgctc aaaccagcaa tgaccacgcc aaagcgatca
     1321 aataccggaa ccgcaatgca tcgcagccct tcttcctgct cttcattatc ttcgccatac
     1381 ccctgctcgc gcacctggtc cagaacggat aataacgctt ctgtactggt gatggtccgc
     1441 tcggtactgc gtttatactc cacgccctca agaatttgca tcacttcatc gcgatcgcgc
     1501 catgccagca gtaccttacc aatcgcggtg ctgtacagcg gattacgacg cccaatccgt
     1561 gaatacatgc gcaaattgta cattgagtca attttgtgga tgtaaacaat actatcttcg
     1621 tccagtgcgc cgaggtggat agtttctttg gtcaggcggg agatctcacg catctggata
     1681 tctgcgctac gaattaaatc gacgttttgc aacgcgcgag cgcccagttc aaacaatttc
     1741 agggtcagcg aatatttctc cgactcccct tcctgcgcca cataacctaa ggttttcatg
     1801 gtctgtaaaa agcgataaac ggtgcttttt gacatcatga cgcgctgcga caactcggtt
     1861 atccctattt cgcgctcttc acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa
     1921 gatacagaat caggctgttt atccagatct gcgttagcca tttatcacct cattgcgagt
     1981 gttttataaa aatcagaact gttttttatt ataatttcgc accagggtgg tcgcaatcca
     2041 tcttttgccg gttagttaca attctgcgac atccaccgtg aatatcagtg ctagaatcat
     2101 acccctgttg attattcacc aaagatataa aattcctatg ccaaaagttc aggccgacgg
     2161 cctgccattg ccccagcgat acggtgcgat attaaccatt gtgattggta tttcgatggc
     2221 tgtccttgac ggcgcaatcg ccaacgtcgc cttgccaaca atcgctacgg accttcatgc
     2281 cacgccggcc agttccatct gggtagtgaa cgcctatcaa atcgccattg tcatctccct
     2341 gctctcgttt tcgtttctgg gcgatatgtt tggctatcga cgtatttata aatgcggtct
     2401 ggtcgttttt ctgttgtctt cactgttctg cgccctttct gattcgctgc aaatgctcac
     2461 ccttgcgcgt gtcatacaag gtttcggcgg tgcagcgttg atgagcgtta ataccgcact
     2521 tatccgcctg atctatccac aacgttttct gggtagaggg atgggcataa actcgtttat
     2581 tgttgccgtc tcttctgctg ccgggccgac aattgctgca gcaatcctct ccatcgcatc
     2641 ctggaaatgg ttatttttaa tcaacgtacc gttgggaatt atcgccctgc ttctggcaat
     2701 gcgctttctg ccacccaatg gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc
     2761 cgtgatgaac gcgttaacct tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg
     2821 gcaatcgctg acattgattg gtgcggaact ggtggtaatg gttgtcgttg gtattttctt
     2881 tattcgccgc cagctttctc ttcccgtacc gctgctaccg gtggatttac tgcgtatccc
     2941 gctgttttca ctttctattt gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat
     3001 ggtttccctg cccttttacc tgcaaaccgt gctcgggcgt agtgaagtcg aaacaggttt
     3061 acttctgaca ccgtggccgt tagcaacgat ggtgatggct ccgctggcag gctatttgat
     3121 tgaacgcgta catgcaggat tgctgggtgc tttagggttg ttcatcatgg ctgcggggct
     3181 tttttccctg gttctgctgc ccgcgtcacc tgcggatatc aatattatct ggccgatgat
     3241 cttatgtggc gctggatttg gcttgttcca gtcacccaat aaccacacca ttattacctc
     3301 cgctcctcgc gaacgtagcg gtggagccag tggcatgtta gggacggctc gtcttctggg
     3361 tcagagtagc ggcgcggctc tggtagcgct gatgctaaat cagtttggtg ataatggtac
     3421 gcacgtttcg ctgatagctg cggctattct ggcagtgatt gctgcctgtg tcagtggttt
     3481 acgtatcact cagccacgat ccagggcata aaaaaagcgc gtcgatcagg acgcactttt
     3541 tagtatttac ttcatattac ttcaggtatt cacccgtacg cagagcttca atacgtttat
     3601 ccagcggcgg gtgagtcatg aacaactcac tgagcgattt cgacttcccg ttaatgcaga
     3661 aagccatcat gctggttgct tcttgcggtt catagctggt tttcaggcgc tgcaatgcag
     3721 caatcatttt ctcgcgacca accagttttg ctgaacccgc atcggcgtgg aattcacgat
     3781 gacgcgagaa ccacatggtg ataatgctcg ccagaatacc aaacaccagt tccagaaccg
     3841 ttgcaaccgc aaagtagatc agcgggttgc cgttgctctc ttcaccttca tcacggtttc
     3901 cgcccataaa acccgcggca agctgcgcca gaatacggga aataaagata acgaaggtgt
     3961 tcaccacgcc ctgaatcagc gtcatggtga ccatatcacc attggcgatg tggctgattt
     4021 cgtgagcaat taccgcctcg gcttcatccg ggctcatgtt ctgcagcaaa ccggtgctga
     4081 cagcaaccag agaggcatca cggcgcgcac cggttgcaaa agcattgatg tccggcgcat
     4141 ggtagatagc cacttgcggc atagcgatcc ccgcctgacg agcctgggtt gctacagtat
     4201 tgaccagcca acgttccctt tcgttacgcg gttgctcgat cacttccccg ccaacagatc
     4261 gtaatgccat ccatttggac atcagaagcg aaacgaagga accaccaaaa ccgaacagca
     4321 aggccatgat catcagcccc tgaacgctgc tcgactgtat ccctgtcagg ctcagtacca
     4381 gcccgaaaac gaccattacg gccaggttcg ttagcaggaa gagcgcgatt cgcatcataa
     4441 ttttcttttt acctcagttt aacaaaacgc aatatgcgat acccacatcg tatgggttac
     4501 gcgactattt tcaagtctgg atagtgcgta agtcaccaga aagacacaac tttacatttt
     4561 gtagcatctg atttacggca tcttgccgct gttaaaaaat caggcacaat ttcttgtgcc
     4621 tgattgatat tacttgacgg gagcgggttg ttccgcgggt ctggcttttt caagcttcgc
     4681 cagatcgagt gcgatattca ccgtctcatc cagataagga tctggctcct ggtaatcttt
     4741 cggtagatca tccagtttct tcaactccgg tttaccttcg cgtttaaagc gttcgttcaa
     4801 acgcgccaga cgcgtcgcat catcttcatt attctctttc tcacgcacag cgtaattcag
     4861 agaaacgata ttgcgcttgt ccttcatagc gttgaagcgc gcgatatcct tcatgatgtt
     4921 ctggaactca ggatctttcg cgatacgcgc attatgttcc ttcagcagct ccggttcaaa
     4981 ggccgttaaa tctcctgatt tcacataagt cgcggcatca atgctatccc acggcagcgc
     5041 gttatcttcg aatttctcac ccgtttccgt ttcttcatta cccgtcggca tgatgatgtc
     5101 tggcgttacg cctttacgtt gcgtactgcc gccgttaacg cgatagaatt tctggatcgt
     5161 gtactgcaca gaacccagcg ctggccattc aggacgtaac atctgatcgt aaatacggtt
     5221 caatgaacgg tactgctgaa cggtgccttt accaaatgtc ggttcaccca caaccagggc
     5281 acgaccgtaa tcctgcattg ccgcggcaaa gatttctgaa gccgaagcac tgaagcggtc
     5341 aaccagcacc accagcgggc ctttatagaa gacctgtccg tcggtgtcgc tgtcttcacg
     5401 aaccttgccg ttgttatcgc ggacctgaac aatcgggccg gatggaatga acagaccgga
     5461 gagcgatacg gcttcagtca acgccccacc accattgcta cgcaggtcga taatcacact
     5521 gctgacattc tgtttttcca gtttctgcag ttgcactttg acatcgtctg tcaaacctac
     5581 atagaagccc ggaatatcca gcacgccgac tttctcttta ccgacggtct tcaccgacat
     5641 tttaaccgcg cggtcttcga gacgaatacg ttcacgggtc aacgttacag tacgcgtctt
     5701 ggtcccttta ccagcaggta aaatttccag acgaacttta ctgcccttag gccctttaat
     5761 taaggccacc acatcatcaa gacgccagcc aatcacgtca accatcggct tgcctgtttg
     5821 accaacaccg acaattttgt caccaacgct gatagctttg ctcttcgctg ccggacctcc
     5881 tgccaccatc gaattgataa cggtgtagtc atcatccatt tgcagcactg cgccaatacc
     5941 ttccagcgac aaactcattt cagtgttgaa ctgttcggta ttacgcgggg aaagatagtt
     6001 ggtatgcggg tcgatttcac gcgcaaacgc cgtcattgcc agcgagaaaa catcttcgct
     6061 gtttgtttgc gccagacgac gaatggcaaa tttgtagcgg cgagtcaggg tttcacgaat
     6121 ttccttatcc gtttttcctg tcagcttcag gcttaactcg tcgaatttga ctttactgtc
     6181 ccacagcgcg ttcaactcag cctcgttttt cggccagggc gctttgctgc ggtcaaggtt
     6241 ataagtgtcg ttgccggtga aatccatcgg cttttccagt accgacaaag cgtactggta
     6301 acgttcaaaa cgacgctttt gcgccagatt gtagagatcg tagaaaacgt cgagtttacc
     6361 tgaacgcagt tcatcgccta actcagtttt ctttttcgcg aactgttcaa catcgctttc
     6421 cagcagcaca ttgtggctgt aatcgagcag attcaggtag cggtcaaaga ttttggccga
     6481 aaatgcctga tcgaggtcga actggcgata atgagaacgg gtgaagcgcg acgttacgcg
     6541 ctcactcacc gtcgcatgct gcgtctcttc ctttaatacc ggaatttgat cagcacgcgt
     6601 gatatcttct acagcgaagg tctggcctgc tattgcaagc aggccagcta acgcggtaag
     6661 cctaaaaaac atgttcatgc ctggcccggc ctccgtttca gaacaccagg tgttctgcgc
     6721 gcacaatcaa agacataccc gaattcagct ggacgcggac gccgtctttg gtgatttcta
     6781 atacggtggc atccatcgcg ttttgacccg ctttcacctt cagggcttgt ccgacagtca
     6841 gagctgaaat gtcagaaacc ggggtgtgct gttcttcgcg aggtgctttt actgtttttg
     6901 gcgctttctc taccggcttt tgcgaacgag gtttacgttc agcgccttct ttgcggcgtg
     6961 gcgtggtcgg acgtggcttg cgttcgcggc gcggtgcgtc ttctttctca ccagcagctg
     7021 cggcagcttc gcgttttttc gcttgctgtt cagcacgctg tgcctgaaca cgcgctttcg
     7081 cttcttcaag ctgcttgcga gcatgctcga catgttgctc gtccagctca ccgcatgggt
     7141 tgccgtcaag atcgacacgt gttgcgccgg gtttaacacc gtaaagataa cgccagctcg
     7201 aagtgtagag acgtaaagcg gatcgtaatt gcgttttgct caggttcatt tccccggcaa
     7261 cacgatcgac caaatcctga aaaataccga ttttcagcgg acgcgcttca ccttccgcac
     7321 tgaaacagtg gggaaaacgt tcggccagaa acgcgattac ttctttactg ctattcaact
     7381 taggttgatt ttccatgaaa tttcctgatt acaacggacg tagccaacaa gcgcaggcat
     7441 gaacaggcgt cattataatg acgctatcag taaatgctac gttatccgtt gattatcctg
     7501 cgacgctcgc aaagaatttt ttgtaatccg tcgttgcaag caccttttca agctgtgcca
     7561 caagctgacg taagccttgc tcgtcctcgt ctgtaaagcg accgaagacg gtactatcga
     7621 tgtcgagaac accaataatc tgatttttca ccaccagcgg cagaacaatt tcagaattac
     7681 tcgtcgcatc acaggcaata tgcccgtcaa acgcatgaac atcctcgata cgctgcactt
     7741 gattgcgggc aaccgcagtg ccgcacacgc cgcgcccgac gggtatccgg acacaggcaa
     7801 ttttgccctg aaatggtccg agcaccagtg tatcgtcctc aagcaaataa aaacctgccc
     7861 agtttacgtc agtgagacgc tcatataaca acgcactggt gttcgcaagc gttgccagaa
     7921 aactggtttc tcctgccatc agcgcgttaa agtcgcgatt taaatccgcg taaaattctg
     7981 ttttgttcat tatataatca cttggttgtc ttaccaggaa ctgccagcct attaaaataa
     8041 gcattaaatg cgttaatgct caagatcatt cccatcatgg gttaagatta atgttaattc
     8101 ttattacatt tggcacgtca tggctcttaa cacaccacaa attacgccga caaaaaagat
     8161 aacagtgagg gctatcggtg aggaactgcc gcgtggtgat taccaacgtt gcccgcaatg
     8221 tgacatgctg tttagcctgc ccgagataaa ttctcatcaa agtgcctatt gtccgcgctg
     8281 tcaggcaaaa attcgtgacg ggcgcgactg gtcgctaacg cgcctggcgg caatggcctt
     8341 caccatgctg ttgctgatgc cgtttgcctg gggcgaaccg ctgttgcata tctggctgtt
     8401 aggcattcgt attgacgcca acgtcatgca aggcatctgg caaatgacca aacagggcga
     8461 tgcgataacg ggatcaatgg tctttttttg cgttattggt gcccccctca ttctggtgac
     8521 ctccatagct tatttatggt ttggtaaccg actgggaatg aatctacgtc cggtactgct
     8581 gatgcttgag cgattgaaag agtgggtaat gctcgatatc tacctggtcg gcattggcgt
     8641 tgcttctata aaggtgcagg attatgccca tatccaggcg ggtgtcggct tgttctcttt
     8701 tgtggcgttg gtgattttaa cgacggtgac gttgtcacat ctcaatgtcg aagaactgtg
     8761 ggagcgattt tatccgcagc gccccgctac gcgtagggac gagaaactcc gtgtctgtct
     8821 tgggtgccat tttaccggct atcctgatca gcgtggtcgc tgcccgcgtt gccatatccc
     8881 gctacgcctg cgtcgccgtc atagtctgca aaaatgctgg gcggcgctgt tagcgtcaat
     8941 cgttttgttg ttacctgcca gcctgttgcc tatttctatc atttatctga acggaggacg
     9001 gcaggaagat acaattcttt ccggaattat gtcgctggca agtagcaaca ttgccgttgc
     9061 gggaatcgtg tttatcgcca gtattctggt accgtttact aaagtgatcg tcatgttcac
     9121 tttactgttg agtattcatt ttaaatgcca gcaaggttta cgcacacgca ttctgttact
     9181 gcggatggtg acctggattg gtcgctggtc gatgctcgac ctgtttgtca tatctttaac
     9241 catgtcgctg attaatcgcg atcagatcct cgcttttact atgggaccgg ctgcgtttta
     9301 tttcggcgca gcggtaattt tgactattct tgctgtggaa tggctggaca gccgcttact
     9361 ttgggatgca catgagtcag gaaacgcccg cttcgacgac tgaagcgcag attaaaaata
     9421 aacgccgtat ctcacctttc tggctgctgc cgttcatcgc gctaatgatt gctggttggc
     9481 tgatttggga cagttatcag gaccggggta ataccgtcac catcgacttt atgtcggcgg
     9541 atggtattgt tccaggccgt acgcctgttc gttatcaggg cgttgaagtt ggaacagtgc
     9601 aggatatcag cctcagcgac gatcttcgta agattgaagt caaggtcagc atcaagtccg
     9661 atatgaaaga tgcgctgcgc gaagagacac agttctggct ggtgacgcca aaggcctcgt
     9721 tggcaggtgt ctccgggctg gacgccctcg tcggtggtaa ttatatcggc atgatgccgg
     9781 gtaaaggtaa agagcaggat cactttgtcg cactcgacac ccaaccgaaa tatcggctgg
     9841 acaatggcga tctgatgatc cacctgcaag cccccgatct cggttcactg agcagtggtt
     9901 cattggtcta tttccgcaag atcccggtgg gtaaagtcta cgactatgcc atcaatccca
     9961 acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg ttttaccgat ctggtgaaaa
    10021 aaggtagccg tttctggaac gtttccggcg ttgatgccaa cgtcagtatc agtggcgcga
    10081 aggtgaaact ggaaagtctg gcggcactgg ttaacggtgc gattgccttc gattcacctg
    10141 aagagtcgaa acctgccgaa gcggaagata cctttggtct gtatgaagac ctggcccata
    10201 gccaacgtgg cgtaataata aaactggaac tgccaggcgg tgccggatta accgccgact
    10261 cgacgccgtt aatgtatcag gggctggaag tcggacagct gactaaactg gatttaaatc
    10321 ctggtggtaa agtcactggg gaaatgaccg ttgatcccag cgtcgtcacc ctgcttcgtg
    10381 aaaatacccg catcgaatta cgtaatccga aattatccct tagcgatgct aatctcagtg
    10441 ccctgctgac cggcaaaacc ttcgaactgg tgcccggcga tggcgagcca cgtaaagagt
    10501 tcgtggttgt gccaggcgaa aaagccctgc tgcaagagcc ggatgttctg acactgaccc
    10561 tgacagcacc agaaagttac ggtattgatg ctggtcagcc gctcattctt cacggcgtgc
    10621 aggtaggcca ggttatcgat cgtaaactca ccagcaaagg cgtcaccttt accgtcgcca
    10681 tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa atttgtcgtc aacagccgcg
    10741 tcgacgtgaa ggtagggctg gatggcgttg agtttctcgg tgccagcgcc tcagaatgga
    10801 tcaatggcgg aatacgtatt ctgccgggcg ataaaggcga gatgaaagcc agctatccac
    10861 tgtatgccaa tctggaaaaa gcgctggaga acagccttag cgatttaccc accacaaccg
    10921 tgagtttgag tgcagagacg ctgccggatg tgcaggcagg atcggtagtg ctgtaccgta
    10981 aatttgaagt tggtgaagtt attactgtgc gtccgcgagc taacgcgttt gatatcgatc
    11041 tgcatattaa gccggagtat cgcaaccttc tgaccagcaa cagcgtgttc tgggcagaag
    11101 gtggggcgaa agttcagctg aatggtagtg gtctgactgt acaggcatcc ccgctctcca
    11161 gagcattaaa gggggctatt agcttcgata acctcagcgg tgccagcgcc agtcagcgta
    11221 aaggcgacaa gcgtattctg tatgcttccg aaacagcggc ccgtgcggtt ggcggacaaa
    11281 ttacgcttca cgctttcgat gcgggaaaac tggcggtcgg gatgccaatt cgctatctcg
    11341 gtattgatat cgggcaaatc cagacgctgg atctgattac cgcacgcaat gaagtacagg
    11401 caaaggcggt gctctatccg gaatatgtcc agacctttgc tcgcggtggt acgcgcttct
    11461 cagtggtcac accgcaaatt tcggcagctg gcgttgagca tcttgatact atcctccagc
    11521 cgtatatcaa cgtcgaacca ggccggggca atcctcgccg cgactttgaa ttacaagagg
    11581 ccaccattac tgattcgcgt tacctggatg gcttaagcat tattgttgaa gcgccggaag
    11641 ccggttcgtt aggtatcggt acacctgtgc tgttccgtgg tctggaagta ggtacggtta
    11701 ccggaatgac gctggggaca ttgtcagatc gcgtgatgat tgcgatgcgc attagtaaac
    11761 gctatcaaca cctggtgcgt aacaattccg tcttctggtt ggcatcgggt tacagtctgg
    11821 actttggtct gacgggcagc gtagtgaaaa ccggcacctt taaccagttt atccgtggcg
    11881 gcatcgcctt cgccacgcct ccgggtacgc cactggcacc gaaagcccag gaaggcaagc
    11941 acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga atggggaact gcgcttccca
    12001 aataatgccc actgctccgg cgtgcctgcg ccggagcgtt tatgctaaac tgcgcgcctg
    12061 tttttttgcc agtggtatat gctcgtggcc caacacaccg tttatttccc ggacgccttt
    12121 ctgacacaaa tgcgcgaagc gatgccttcg acgctctctt ttgatgattt tcttgccgcc
    12181 tgtcagcgcc cgttgcgccg cagcattcgc gttaatacgc tgaaaacctc cgttgctgat
    12241 ttcctgcaat taactgctcc ttatggctgg acgcttacgc caattccgtg gtgtgaagaa
    12301 ggtttctgga ttgaacgcga cagtgaagat gcattgccgt tgggtagtac cgccgagcat
    12361 ttaagcggcc tgttttatat tcaggaagcc agttcaatgt tgcctgttgc cgccttgttt
    12421 gctgacggta atgcaccaca gcgggtgatg gatgtcgctg ctgcaccagg ttccaaaacg
    12481 acgcaaattg ctgcgcggat gaataacaaa ggggcaatcc ttgccaatga gttttccgcc
    12541 agtcgggtaa aagtgttaca tgccaatatc agccgctgtg gcatcagtaa tgttgcgctc
    12601 acgcattttg atggccgcgt gtttggtgcg gcagtgccag aaatgttcga tgccattttg
    12661 ctggacgctc cctgctccgg cgaaggcgtg gtgcgtaaag atcccgatgc gctaaaaaac
    12721 tggtcaccag aaagcaatca ggaaatcgca gcaacccaac gggagctgat cgacagcgcc
    12781 tttcatgcat tacgtcctgg cggtacgctg gtttactcga cctgtacctt aaaccgggaa
    12841 gaaaacgaag ccgtttgcct gtggctgaaa gagacttacc acgacgcagt agagttttta
    12901 ccgcttggcg atctcttccc tggtgcaaat aaggcgctga ccgaagacgg ctttttgcat
    12961 gttttcccac aaatttacga ctgcgaaggc ttcttcgttg ctcgtctgcg taaaactcag
    13021 gcgatccccg tcttacccgc cccaaaatac aaagtgggca atttcccgtt tagcccggtg
    13081 aaagatcgcg aagccggtca aattcgtcag gcggctgcaa gtgttggctt aaactgggat
    13141 gaaaacctgc gactctggca gcgtgacaaa gaactgtggt tgttcccggt aggcattgaa
    13201 gccctgatcg gtaaagtccg attttctcgc ttggggatta aacttgccga aacgcacaac
    13261 aaaggttatc gctggcagca tgaagccgtt attgcccttg cctcccccga caatgtgaac
    13321 gcttttgaac tgacaccgca ggaagcggaa gagtggtatc gcgggcgcga tgtttacccg
    13381 caagccgcgc cagtggcgga tgatgtattg gttactttcc agcatcagcc gattggttta
    13441 gccaaacgga ttggttcacg actgaaaaac agctacccgc gtgaactggt gcgcgacggg
    13501 aaacttttta ccggtaacgc ctgacagcgc acaaaaaaag cgcacttttt gactggcaca
    13561 ttcgactgcc tcaactaggc tgaaaaatgg tgcgatcgga ctggtcgtac cacaatcggc
    13621 agctaaatgg agagcacgaa gatgaaaacc agtgtgcgca taggcgcttt tgaaatcgac
    13681 gacggcgaat tacacggcga atcgccgggt gatcgaacgt taaccattcc ttgtaaatca
    13741 gaccccgatt tatgtatgca actggatgcc tgggatgctg aaaccagtat ccctgccctg
    13801 ctaaatggcg aacactctgt cctttaccgt acccgttacg atcaacaatc tgatgcctgg
    13861 attatgcgtc ttgcctgatc caaaaagaac ccgtcggcat ggcgggttat ttgtccgggt
    13921 tattcccccg ttgtaaaatc tctcctaaac ttaacggtac ggcaccacac ttcggggatg
    13981 aaatgttcgc gctggtactt tttgtttgct acctggatgg cggttgtgaa gatattgttg
    14041 tggatgtcta caacacggaa cagcagtgtc tttattctat gagcgatcaa cggatccgcc
    14101 atggcggttg ttttccgatt gaggatttta tagatggttt ctggcgacct gcacaggagt
    14161 acggtgattt ttaattattg caattgcaca agagtcagtt ctcccccaaa gacagcaccg
    14221 gtatcaatat aatgcaggtt gccaatatcc acgcgatggc gcaaaggtgt atgaccaaac
    14281 cagaaatgat cagcacctgc aattccctgc cctttttggc gttcacctaa tcgcgagcgg
    14341 ctccacaaga cctgatgcaa atcgacgtcc ttttgccatt catagacatc atctggataa
    14401 tcggcatgag caataacatg tttgccggtg cgactgtgta attcaagaat aaagggcaaa
    14461 tgctgacatt tttccagcgc cgttttcgct tgtttctgtt gattatctgc cagcgcaata
    14521 aaccagtcgc cgccatttat caaccataaa gacatctgcc gggatgccag cgcatccatc
    14581 gccatctgtt catgattgcc tcttaccgca cgaacccaat gttgttccag taactgcagg
    14641 caacgtaaac tttgcggccc acgatcgata acgtctccca ctgagataag taaatctcgc
    14701 cacggatcaa aacgacaatg ccataatttg cggcgcaact gctcaagaca accgtgtata
    14761 tcgcctgaaa gccagatatg tcgccattga tgacccgcaa ttctctgata aacgggcgca
    14821 ggctgtttca tcaatatttt cctcccgcgc taaagatcac ataattttaa caagaatgtt
    14881 aaaaaacgct ggactcagac agtagagtgt gtgttatggt tgactataaa gtcagcgaag
    14941 ggaatgcttc tggcttttaa cagataaaaa gagaccgaac acgattcctg tattcggtcc
    15001 agggaaatgg ctcttgggag agagccgtgc gctaaaagtt ggcattaatg caggcttagt
    15061 tgccttgctc tttaagaata gatgacgacg ccaggttttc cagtttgcgt gcaaaatggt
    15121 caataaaaag cgtggtggtc atcagcttaa atgttaaaaa ccgcccgttc tggtgaaaga
    15181 actgaggcgg ttttttattg gaaatcaaaa ggctatttta ggtaattaac agagtttttc
    15241 agctcgctct ataaacggtg ccagactcat tttttcgccg ggattgttag gatcatcaat
    15301 ctgaatcacc gaaatgggtt gggctttagt cttcccactg gcaacttcct tttgtgcgat
    15361 atcgtttaaa ggatactgca cgagggtact tgggttaatg acatacaaag cattacccgg
    15421 tcggcaagtc agcatcacct cttcgcgatt aaacgcccat ttgtctttac ccacttcaaa
    15481 acggctgacg gtaatcacct gcggtgccgc cagcgcagct gcagaactgg tgagtaacag
    15541 aaacgccaga atactttttc tcatcatttt cgtaatccat caaaaaggtt cgagagtcgc
    15601 aagcaggctg atgatcacca gcactaccgc acctatcgcc cattcgagtt tagtcatcca
    15661 gacaaaccag ggagccgcac gatcttcatc ctgcctcatg cgcggtacaa gaacataacg
    15721 attagccagc gcgatgacca ccataatcat caccaggatg gctttgagca ataagagttg
    15781 gccccagtaa gtcgtaagcg tggggggaaa tccggtaatc aacaatgcat taagcacgcc
    15841 gcttgccagt acgccgatca ccgcaaaatg cccgcaccag gaaaaacgca tcagcgcctg
    15901 aatagcctgg tgtcgccaac ggcctttgat gagttgcata caccagagca ccggcagtaa
    15961 tcccccaaac cacgccgccg cacagatcag gtgaattgcg tgattagtct gatggatttt
    16021 cgccgttacc ccttcattca acgtcgcatg cccgactccc gccagcagaa taaattgcgc
    16081 ggtagtgagc ataaacagca aacgtggcat attccgcggt tgcataaggg cgacgatcaa
    16141 cgttacgagg gcgagaacaa tttgccataa ccagacgcca ccaaactgcg tttgcaaaac
    16201 agccaaccaa atgtttggcg aaaatacatc tgaccatccc gctcccatca acccgccctg
    16261 aacggcgagc attgcagtgg cactgatcaa actccagacg gcagcgtgct gttgtaaacg
    16321 cagaaaacgc ttcgtcagca agcgacgaat cgttaagggg gccagccagg caccgtacat
    16381 tgcgaagcca aaaaccagca ttagcgaggt gaaatggata aatcgcagcg cgatccaggt
    16441 aaacgccagc atgatttatt tcacactaaa ggtgtaatgt cctttcgttt tatgcccatc
    16501 caccgaaaca acatgccagt caacggtgta ggttccgggt ttcagcgaat cggccagtgg
    16561 aacaatcagt tgcttttggt cctgctcatt ccgcttcgcc ggtaatgttt taatattttc
    16621 gttttttggc cccgtgattt ttgcaccact gaatcctgtt tcaacgcctt ccgagaagtt
    16681 taaagtgatt gcctgcggag ctgctgtcac ttgtgcattt gccgcaggat actgatgcgt
    16741 taaatgcgca tgtgcccaga cggaaggtgt caccaacgaa gtggtcagga ttgccagcgc
    16801 gtagcgaagg gagcgtgcag ttgaagccat attatctatt cctttttgta ataacttttt
    16861 tacagagcat aaccttgtct aatgtctgag tcgaggatca tcaattccgg cttgccatca
    16921 aggctcactc ttagtaactt ttgcccgcga atgatgagga gattaagaat gctgaagaat
    16981 ctggctaaac tggatcaaac agaaatggat aaagtgaatg tcgatttggc ggcggcaggg
    17041 gtggcattta aagaacgcta caatatgccg gtgatcgctg aagcggttga acgtgaacag
    17101 cctgaacatt tgcgcagctg gtttcgcgag cggcttatcg cccaccgttt ggcttcggtc
    17161 aatctgtcac gtttacctta cgagcccaaa cttaaataaa acttatacat agttacactt
    17221 tcttacataa cgcctgctaa attatgagta ttttctaaac cgcacccata atttgcagtc
    17281 attttgaaaa ggaagtcatt atgtcgttct ggaaagttgc agcagcgcaa tatgagcccc
    17341 gtaaaacctc actcaccgaa caggtagctc atcatctgga gtttgtgagg gccgccgcca
    17401 ggcaacaatg tcagctgtta gtttttccat cgctttcttt actggggtgt gattattcgc
    17461 gacgtgccct tcctgcccca cccgatctct cgctattaga cccgctttgt tatgccgcaa
    17521 cgacctggcg aatgaccatc attgccggcc ttcccgttga atataacgat cgctttattc
    17581 gtggaattgc ggtattcgca ccgtggcgaa aaacgccagg gatctaccat cagagttatg
    17641 gtgcatgtct gggccgtcgt tccagaacca tcacggtggt cgatgaacaa ccgcaaggta
    17701 tggatatgga cccgacctgt tcactgttta cgacagggca atgtctggga gaacccgacc
    17761 tgctggcgtc ggcccgtcgc ttacaatttt tttcacatca gtactcgatt gcagtgctga
    17821 tggcaaatgc ccgtggtaac agtgcactat gggatgaaca tggtcgtctc atcgttcgcg
    17881 ccgatcgtgg ttcattattg ttagtcggtc agcgttcatc acagggttgg caaggcgata
    17941 tcattccatt acgctaggct tttcggcctg gagcatgcca tgttgcgcat tatcgataca
    18001 gaaacctgcg gtttgcaggg agggatcgta gagattgcct ctgttgatgt cattgacgga
    18061 aaaatcgtca atcccatgag ccacctggtg cgtcccgatc gtcctattag tccacaggcg
    18121 atggcgattc atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg
    18181 atcccgcact attacggtag tgaatggtat gtcgcgcata acgccagctt tgaccgccgc
    18241 gtactgcctg aaatgcccgg tgagtggatt tgcactatga aactggcccg tcgtttgtgg
    18301 ccagggatca agtacagcaa tatggcgcta tataaaacac ggaagctcaa tgtacagacg
    18361 ccgccgggcc tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt
    18421 atcgatatta tgaacacctc tggctggacg gcagaacaga tggccgatat caccggacgt
    18481 ccatcgttga tgacgacctt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt
    18541 gccgaacgcg atccgggcta tctgcgctgg ttgtttaata acctcgacag catgagcccg
    18601 gagctgcgtt taacactgaa acattatctg gaaaatactt aatccgctga ctgagcgggt
    18661 aatgttccct gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat
    18721 ttgaagcgac cagatttacc gccatgacct gagtccatgt cggtacagag caataaaaga
    18781 tggtcatcgg ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc
    18841 acctgagaat cgtgtaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg
    18901 ttgtcatacg ggctgtagct tttcatatac tcgtaatatt gcggatcctg cggattcccc
    18961 cactcttcaa attcgccggt ggtaagagga attgattcat ccagcattgt tgttacaaca
    19021 tcaacaaacg gtacctgggc gataacgccg tggaacaatt ccgggcgttg attaatcgca
    19081 acgcccatca acataccccc cgcactcccg cccatcgcat aacagagcga aggagagcca
    19141 tagcccagtt ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttc
    19201 ttcagaaatt taccgtcctc gtaccattgt tgccccagct caccgccgcc acgaacatgg
    19261 acaatggcgt agacaaatcc acgatccaac aaactcaagc ggctaaaact gaaatcggca
    19321 tcaatacttg cgccgtaaga accgtagcca tacaccagca gcgggttgtg tcctttgcga
    19381 aaatgtttgc gatgatagac cagcgaaacc ggaacttcga cgccatcacg ggcgactatc
    19441 cacaggtgtt cactgcggta attcgccgca tcaaaaccag gaacttccgt ttgttttaat
    19501 acacgacgct caccggtatc catatccagt tcaaacaaag tatctggtgt ggtcatggac
    19561 gaataaccat aacgcaatcg cgcggtttca ggttctggat tgtaggcaat ccaggtcaca
    19621 taggccggat catcaaaggc aatgccaatg acttcccggg tcttgcggtt aatttgccgc
    19681 aaactggtta acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac
    19741 ccttccagca taatgttatc acgtggcgga attaactctt cccactgttg ctcatcacgc
    19801 atacgggtac ggtataagcc aaagttttta ccgtggcggt tggaacgcag ataaaaccga
    19861 tgctggtagt gatcaaggct gtattcgtga tctttgcggc gaggcagaaa aacaaacggc
    19921 tcggcatcgg ccatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaga
    19981 tgaatgacca cataatgctt cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct
    20041 ttttcttcgt aaatcagttt atcttgcgat gctggcgtac cgatggcgtg acgccagacc
    20101 tgataaggca gcagcgtcac tggatgcttg cgaacatagt agaaagtcca ggagtcattt
    20161 gcccagacaa agctgggttc aacgttatcc agcagttccg ggtaccagtt acctgtttcc
    20221 agattacgaa aacgaatgcc gtactgccgt cgggaaagaa aatcttctgc cagtgccata
    20281 atggtgttat ctggcgtaat cgccattccg cccatcgaat aaaactcact atgagccgcg
    20341 cgcttgttgg catcgagcaa tgtttcccac tcgtcccact cttcactgaa cgccgattga
    20401 cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt
    20461 ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg
    20521 cgatcctgca aggcttgttg cgaggccatc acccgatgac cgtaactatt ttcttgttgc
    20581 agatagtcca ggactttcgg ctgagaacgc gtatcgtccc gcagccagta gtaattatcg
    20641 atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg
    20701 ttattgttct ttctggttga aacatcttat aagggtggca aaactcaccg gggatgcaag
    20761 cgaaacaggg tagtcattgc ttagatgatg acaggtaatg gcgcggatat cgactgttat
    20821 gcagacagag aaaatcagcc tgttcaaatg gctgtgcggt tctggataac ccgaaatagt
    20881 caacttcagg ctatccagag agcggaatta ctccgccaaa gtacgttttt gctgttcgag
    20941 atcgcgttca atgccttcac gaacatcctg ggggattttc agcgcatcac ccagtgcatt
    21001 caggtaactg cgttccataa aatggtcaat atcaatagcc gcgcagctca ggaaatagat
    21061 ttccagcgcc tcttcttcat tgcggacccc ggtagccagg cgttgtggat ccagcggttg
    21121 ttcgattgcc tgctcaatga gtacacgccc cttctcttcc acaccggctt ctcgcaattg
    21181 ctggtcgatt gccgcacgtt ctttggcatc aatatggcca tcacttttag cggcaaagac
    21241 cagcgcaagg atcaaacgtt ccgtacgctc atccagcggc gtactttgcg cgccaaactg
    21301 cggttcatcc tgatgcgccg cgcgaatttt atctttgtat ttattccaca gcaccgtacc
    21361 cgctaccgct ccgccgccaa ccagtaacgc gtttgtgcca tatttcgtaa gaagtttacg
    21421 tgctgattta tttgcgacca gcagcccagc cagtccgcct aatgcgccag gcactaaaag
    21481 tttgcccaat ccctgatccg cagacgagga ggtagaagaa cggctttgcc caagcaggga
    21541 ttgcagttga tttaaccagt tagccatatt tgctcctcaa taaccatttt tagtgcctgt
    21601 agcatagcgg atgaggatgt cagaaaatgt atattgagac aaaagatgcg caaataagcg
    21661 gggcggtagg ccgttattca aagaaattcg cgccaggtaa ggtgttcact gtatataagg
    21721 tgataagggt attttaggtg aaagtagttt gacgttttga gtgaaactgt ttgatgctga
    21781 ttgcccaatg cgacgctgtt gcgtcttctc aggcctacga ttatgttgcg ttgtagggcg
    21841 gacaaggcac ccgcgccgca tccgccaacg gtcaatcgaa ttatcagaag ccattaacgc
    21901 cgctgatatt ccgccattcc cgctttgcag tccacgctga cctggtaatg aatatcggca
    21961 cttttaccac gcacggttag cggtactgac catttatcat ctttcccctg aatgtcctgc
    22021 aaactgaccc atgccacggg gtcggcctga ccgacaattt tttgatcatc agcccagcgc
    22081 gccacgcgat tttgttggta atcacgtttt acgctcgcgg caattccggc agcatccaga
    22141 ccttcacact ttgggaaagt gaccgacttg ctggtttcat tattggcagc gaaaactgat
    22201 gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc tttttttcat gtttttctcc
    22261 atagcacaat gattcaggag aaagcatggt acaaattgtc aggagcgcaa gttgcttcag
    22321 gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga cgacctgtgg ggacggtaat
    22381 ttgccggttt tcagaatggt gctcagcaca tctttttgtt ctgccagcca tagcgaaagg
    22441 gcttcacgtt gctcatcttc catttgcact ggagaatttg tcagccaggt gtcaagcaga
    22501 tcggccgtat caagcatctt gtcataagca tcggcttcct ttttgctggt aaacgacatt
    22561 ttctcttcgc cctcacgaat gactacgtat ttaacttcaa ccgccatttg cagcctctca
    22621 taataactgt gattttatac agtatatttc ttttcagttg ggaaatcaac atcagcaata
    22681 aagacacacg caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg gatattcgtt
    22741 atgacgttat taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt attaggctcc
    22801 ggtgaactgg gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc
    22861 gtcgatcgct atgccgacgc accagccatg catgtcgcgc atcgctccca tgtcattaat
    22921 atgcttgatg gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg
    22981 ccggagatcg aagctattgc caccgatatg ctgatccaac ttgaagagga aggactgaat
    23041 gttgtcccct gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg
    23101 gcggcagaag agctgcagct gcccacttcc acttatcgtt ttgccgatag cgaaaacctt
    23161 ttccgcgaag cggttgccgc cattggctac ccctgtattg ttaaaccggt gatgagttct
    23221 tccggtaaag ggcagacgtt tattcgttct gcagagcaac ttgctcatgc atgggagtac
    23281 gctcagcaag gtggtcgcgc cggagcgggc cgcgtaattg ttgaaggcgt cgttaagttt
    23341 gacttcgaaa ttaccctgct aaccgtcagt gcggtggatg gcgtccattt ctgtgcaccg
    23401 gtaggtcatc gccaggaaga tggcgactat cgtgaatcct ggcaaccaca gcaaatgagc
    23461 ccgcttgccc ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc actgggcggt
    23521 tatgggctgt ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt cagtgaagtc
    23581 tcccctcgcc cgcatgacac cgggatggtg acgctaattt ctcaggatct ctcggagttt
    23641 gccctgcatg tacgtgcctt cctcggactt ccggttggcg ggatccgcca gtatggtcct
    23701 gctgcttctg ccgttatcct gccgcaactg accagtcaga atgtcacgtt tgataatgtg
    23761 cagaatgccg taggcgcaga tttgcagatt cgtttatttg gtaagccgga aattgatggc
    23821 agccgtcgtc tgggggtagc actggctact gcagagagtg ttgttgacgc cattgaacgc
    23881 gcgaagcacg ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca
    23941 aaaatgcccg atcctcgatc gggcattttg acttttacag cttagcgcct tctacagctt
    24001 cacgcgccag cttagtaatg cggtcgtaat cgcccgcttc cagcgcatct gccggaacca
    24061 gccaggaacc accgatgcac agcacgcttt tcagcgccag gtagtcacgg tagttagccg
    24121 gagaaatacc acctgtcggg cagaaacgga cctgagagaa cggacccgcg atcgcctgca
    24181 gggctttcac accgccgtta gcttcagccg ggaagaattt gaactctttc aaaccgtagt
    24241 ccatacccag catcagttcg gaaacagtgc tgatccccgg aatcagagga atagtccctt
    24301 cggtagcagc tttcagcagc ggctcggtca gacccgggct aattgcaaac tgtgcacctg
    24361 cttcagtgac ttctgccagt tgctgtggat tcagcaccgt accggccccc acaatcgctt
    24421 caggcacttc tttggcgata gcacggatag cgtcaactgc gcactcggta cgcagagtca
    24481 cttccagaac gcgcacccca ccagcaacca acgcttttgc catcggcacc gcgtgttcca
    24541 gtttttttac cacgataacc ggtacaaccg ggccggtggt caggattgat tctgcacttg
    24601 ttttccagtt tttcatcaga gttttctctc gcctgattac aaatttgtcg tcttaaaaag
    24661 tgatacaggt tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg ctgaataatt
    24721 cacgtcctgt tcccacgcgt gacgcgctca ggtcaggaat gtgcggttcg cgagcagcca
    24781 gttccgcttc gtctaccagc agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt
    24841 ccccgtcgcg cacttttgcc agcagcccgc catcgtaggc ttctggtgtt acgtggatag
    24901 ctgacggcac tttacctgaa gcgccggaga gtcgtccatc ggtaactaac gcaattttga
    24961 aacaccggtc caataataca ccaagtggcg gcatgagttt atgtaattct ggcattccgt
    25021 tcgcttttgg cccctgatga cggacaacaa cgacacaatc gcggtccagt aaacccgctt
    25081 caaaggccgg cataacgtca tgctggcttt caaaaacaac cgctggcgct tcaatcacct
    25141 ggttctctac cggcacggca gaggttttca taaccgctcg gcccaggtta ccgcttaaca
    25201 cttttgtccc accatgatga gagaaaggtt gttcgaagga cgcgatcaca ttgttgtcga
    25261 gtgatttttc tgccccttcc cgccagtcca gttcaccatt attcagccat ggttcaaggg
    25321 tataacgaga cagaccaaag cctgccaccg tattgacatc ttcatgcagc aggcctgctt
    25381 tgagcagttc acgtaccaga accggtacgc cacctgccgc ctggaagtgg ttaatatcgg
    25441 ccggaccgtt cgggtagaga cgtgccatca gcggtacaac atcagaaagg tcagagaagt
    25501 catcccagtt aatctgaata ccggctgcgc gcgccatcgc caccaggtgc atggtgtggt
    25561 tagtggaacc accggtcgcc agcagtgcaa cgataccgtt caccaccact ttctcatcga
    25621 tcatcttacc gatcggcatc cattcattac cattaccggt catgcgcgta acctggcgcg
    25681 cagctgcagc ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg
    25741 gcaactgcat ccccataaac tccaccacca tctggttggt gttggcagta ccgtagaaag
    25801 tacatgttcc cggcgcatga taagacgcgg cttctgactc cagtaatgcc atgcggtcca
    25861 ctttaccttc ggcataaagc tggcgaatac gcactttttc tttatttggc aaaccgcttg
    25921 ccatcggtcc agacggcaca aacaccgcag gcaaatgacc aaacgacagg gctgccatgg
    25981 tcagacctgg gacaatcttg tcgcacacac cgagaaacag agcaccatca aacatgttat
    26041 gggacagccc caccgccgca gacatcgcaa tcacttcgcg gcttaacagc gacaattcca
    26101 ttccatcctg cccctgggtg acaccatcac acatcgccgg aacaccgccc gcaacctgac
    26161 caaccgcatt agcttcatgc agggctttac gaatgatttc cggatagtgt tcataaggct
    26221 ggtgagcgga gagcatgtcg ttataggagg tgatgatggc gatattgtta cgcaacatgc
    26281 ttttcaaaga ggctttgtct tctggctggc aggcagcgaa accgtgtgcc aggttaccgc
    26341 atgccaactg cgaacgatga acggtcgaag ttttcgcttg ttctatccgg gcgaggtaag
    26401 cagagcgagt ctcgcgcgaa cgttcaatga ttcgatttgt tacgcgtaac aattgtggat
    26461 tcataaaggc tcctgaaatt gagttgtcag agcaggatga ttcacaacac gtttcattca
    26521 gaggattcat gactgaaacg cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag
    26581 tgtaataaaa aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc
    26641 tacaatctgt gcaagatcat gttaccggta aaataaccat aaaggataag cccagatatt
    26701 actcaaactc attccaggaa cgaccgtcac gggtaatcat cgccaccgag gcaacggggc
    26761 cccaggttcc ggcctgatac ggtttcggcg catcattgtc catcgcccac gcctcagtaa
    26821 tggagtctac ccatttccag gcttcttcca cttcgtcgcg acgtacaaac agtgcctgaa
    26881 taccacgcat ggtttccagc agcaaacgtt cataggcatc cgccagatgc gtctgattaa
    26941 aggtttctga atagctcaga tccagcttgg tgatttgcag gttatgtttg tggtcaaggc
    27001 caggaacttt attcagtacc tggatatcca cgccttcatc aggttgcaga cggatagtca
    27061 gtttattctg cggcagatcc tgccacgact ctttaaacag attcagttca ggtgttttga
    27121 aatagaccac gacttcagaa catttggtcg gcagacgttt accagtacgc aggtagaatg
    27181 gcacaccggc ccagcgccag ttatcaatgt cgacgcggat cgccacgaag gtttctgtat
    27241 tgctgctctt gttcgcgccc tcttcttcca gatagcccgg cacttttttg ccctgagcga
    27301 agcccgcagt atattgcccg cgtacggttt tttcgcgcac gttggagcgg tcgatgcggc
    27361 gcagagactt cagtactttc actttttcat cgcggatgct gtctgcgctc aggtcagacg
    27421 gcggagacat cgcaatcatg caaagaattt gcagcaggtg gttctggatc atgtcgcgca
    27481 tctgaccggc tttatcaaaa tagccccagc gcccttcgat ccccacttct tctgccacgg
    27541 taatctcaac atgatcaatg gtgcgattgt cccagttgtt cacaaacaga gagttagcaa
    27601 aacgcaacgc caacaggttc agcactgttt ctttaccaag atagtggtcg atacggtaaa
    27661 cctggcactc ctcgaagtat tcgccaacct gatcgttgat ttcctgcgag gtcgccagcg
    27721 acgtccccag cggtttttcc atgactacgc gtgccggttt agcattcagt tttgcctcac
    27781 caagcccttt gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga
    27841 tacgattttt ttgatccagc atcgcgccga gacggctgaa tgcagcagtg tcattgacat
    27901 caagattaca aaaatccaga cgtgcactca gggtgtccca taaaccttca tcaatggttt
    27961 ctttcatgaa agtttcgagc gcctcgcgga caactttggt ataagccgct ttatcccagt
    28021 cagcacgccc tacgccgata atccgggtgt ccgggttgag ctgaccggct ttttccagtt
    28081 gatacaggga aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca
    28141 ggtcacaggc ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt taactaaccc
    28201 ggtacttaag ccagggtata cttgtaattt tcttacggtg cactgtactg cttttacgag
    28261 cttgcgaaaa ctgtaaacgc ttatccaccc gtgcgattac ggtaaaagcg cgcaaagtgc
    28321 ggcaaaactg ataaaaaaat catcgttttc cattgggtaa aaatctgaca ctgatcatgt
    28381 tatgaaaaaa aataacaact tttttatctg cttttgtcat taacacagca cacaggcgta
    28441 atatctgaca aaactgcatt tcgatttctt tcagagcgga aatcgtcatt acccgtgagt
    28501 ctctttacat catgaatatg ctggaaaaaa tccagtctca gctggaacat ttgagcaaat
    28561 ctgagcgcaa agttgccgag gtcattctgg cttcgcccga taacgcgatc cattcgagta
    28621 ttgctgctct ggcgctggaa gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca
    28681 gcatggacac acgcggtttt cctgatttta aacttcatct ggcacaaagt ctggcgaatg
    28741 gcactcccta tgttaatcgc aacgtcaatg aagatgacag cgttgaatca tacacgggga
    28801 aaatatttga gtccgcaatg gcaacgctcg atcatgtccg tcattcactg gataaatctg
    28861 ccatcaaccg cgccgtcgac ttgctcactc aggcaaaaaa aatcgccttt ttcggattag
    28921 gttcttcagc cgccgttgcc cacgatgcga tgaataagtt ctttcgtttt aatgttccgg
    28981 tggtgtactc cgatgatatc gtgctgcaac gcatgagttg tatgaattgt agcgacggag
    29041 acgtggtggt gctgatttct cacacgggaa gaacaaaaaa tctggtcgag ctggcgcagc
    29101 tggcacgcga aaacgacgct atggtaattg ccctcacctc tgcgggtacc ccactcgccc
    29161 gggaagcaac gctggcaatt accctcgacg taccggaaga tactgacatt tatatgccca
    29221 tggtttctcg acttgcacaa ctgaccgtga tagatgtgct ggcgacagga tttactttgc
    29281 ggcgcggtac aaaattcaga gataacttga agcgggtcaa ggaagcgctg aaggaatcgc
    29341 gttttgataa gcagttactt aatttaagtg acgatcgcta aaaacgactg tcactgtcct
    29401 aatcttatac gacatccgaa tgagattaat ttatcgccat cacggcgtta tttcattcgg
    29461 atttcatgtt caagcaacac ctggttgttt cagtcaacgg agtattacat gtccagaagg
    29521 cttcgcagaa ctaaaatcgt taccacgtta ggcccggcaa cagatcgcga taataacctt
    29581 gaaaaagtta tcgcggcggg tgccaacgtt gtacgtatga atttttctca cggctcgcct
    29641 gaagatcaca aaatgcgcgc ggataaagtt cgtgagattg ccgcaaaact ggggcgtcat
    29701 gtggctattc tgggtgacct ccaggggccc aaaatccgtg tatccacctt taaagaaggc
    29761 aaagttttcc tcaatattgg ggataaattc ctgctcgacg ccaacctggg taaaggtgaa
    29821 ggcgacaaag aaaaagtcgg tatcgactac aaaggcctgc ctgctgacgt tgtgcctggt
    29881 gacatcctgc tgctggacga tggtcgcgtc cagttaaaag tactggaagt ccagggcatg
    29941 aaagtgttca ccgaagtgac cgtcggtggt cccctctcca acaataaagg tatcaacaaa
    30001 cttggcggcg gtttgtcggc tgaagcgctg accgaaaaag acaaagcaga cattaagact
    30061 gcggcgttga ttggcgtaga ttacctggct gtctccttcc cacgctgcgg cgaagatctg
    30121 aactatgccc gtcgtctggc acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt
    30181 gaacgtgcgg aagccgtttg cagccaggag gcaatggatg acatcatcct cgcctctgac
    30241 gtggtaatgg ttgctcgcgg cgacctcggt gtggaaattg gcgatccgga actggtcggc
    30301 attcagaaag cgttgatccg tcgtgcacgt cagctaaacc gcgcggtaat cacggcgacc
    30361 cagatgatgg aatcaatgat tactaatccg atgccgacgc gtgcagaagt catggacgta
    30421 gcaaacgccg ttctggatgg tactgacgct gtgatgctgt cggcagaaac tgctgctggt
    30481 caatacccgt cagaaactgt tgcagccatg gcgcgcgttt gcctgggggc ggaaaaaatc
    30541 ccgagcatca acgtttctaa acaccgtctg gacgttcagt tcgacaatgt ggaagaagcc
    30601 attgccatgt cagcaatgta cgcggctaac cacctgaaag gcgttacggc gatcatcacc
    30661 atgaccgaat cgggtcgtac cgcgctgatg acctcccgta tcagctctgg tctgccaatt
    30721 ttcgccatgt cacgccatga acgtacgctg aacctgactg ctctctatcg cggcgttacg
    30781 ccggtgcact ttgatagcgc taatgacggt gtagcagctg ccagcgaagc ggttaatctg
    30841 ctgcgtgata aaggttactt gatgtctggt gacctggtga ttgtcaccca gggcgacgtg
    30901 atgagtaccg tgggttctac taataccacg cgtattttaa cggtagagta agtacgttgc
    30961 cggatgcggc gaaaacgcct catccggcct acagttcaat gataattcaa cagatttcga
    31021 atcttctgaa gcaaacttga acttatcatc agtcgaaggc ctctcctcgc gagaggcatt
    31081 ttttatttga tgggataaag atctttgcgc ttatacggtt ggatttcgcc cggtttgcga
    31141 gttttcagca attttaatat ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg
    31201 acttcttcat tcatccgccg cgcaatcgta tgatcatccg cctctaacag atcatccatc
    31261 ggtgggcgca cctgaatcgt cagacgatgc gtcttgccat cataaatcgg aaacagcggt
    31321 acaacgcgcg cacggcacac tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag
    31381 gtggcaaaga aatcaacaaa ttcgctgtgt tctgggccat gatcctgatc gggtaaataa
    31441 tatccccagt aaccctgacg taccgactgg atgaatggtt taataccatc atttctcgca
    31501 tgcagacgac caccaaagcg acgacgcacc gtgttccaga cataatcaaa aaccgggttg
    31561 ccctgattat ggaacatcgc tgccattttc tgcccttgcg aggccatcag catggcagga
    31621 atatcgacgg cccaaccgtg cggcaccaga aaaatcactt tctcattatt acgccgcatc
    31681 tcttcgatga tctccagccc ttgccagtca acgcgcggct gaattttctc cggcccgcgt
    31741 attgccaact cagccatcat taccatcgct tgcggcgcgg tggcaaacat ctcatctaca
    31801 atcgcttcgc gttcagcttc actacgttct ggaaagcaga gcgacagatt gattaacgca
    31861 cgacggcgtg agctttttcc cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga
    31921 tcacggaact ttggcggcgt taaagcgata cccgccatcg ctgctacgcc cagccatgct
    31981 ccccagtagc gcgggtggcg aaaggattta tcaaactcag gaatgtattc gctattattt
    32041 tttttcgtat ccatgctttt ccagtttcgg ataaggcaaa aatcaatctg gtgatagtgt
    32101 agcggcgcaa cttgccccgc accaaataaa aaagccggta ctgactgcgt accggctgcg
    32161 aatggatgtt aattaatcaa accgtagctg cggcacaatc tctttggcct gtgccaggaa
    32221 ttcgcgacga tcggagccgg tcagcccttc ggtacgcggc agttttgccg tcagcgggtt
    32281 tacggcctgc tggtttatcc atacttcata gtgcagatgc ggcccggttg aacgtccggt
    32341 attaccggaa agcgcgatac ggtcgccacg tttcaccttc tgtcccggtt tcaccaggat
    32401 cttgcgcaag tgcatataac gcgtggtgta gctgcgacca tgacgaatag ccacataata
    32461 acctgctgcg ccactacgtt tggcaaccac cacttcaccg tcacccactg aaagcactgg
    32521 cgtaccttgt ggcatggcaa aatcaacacc tctgtgtggc gcaacgcgac cggtcaccgg
    32581 attagtacga cgcgggttaa agttagatga gatacggaac tgtttcgccg tcgggaatcg
    32641 caagaatcct ttcgccagac cagtaccgtt acgatcgtag aatttgccat cttcagcgcg
    32701 gattgcgtaa taatctttac cttctgaacg caaacgtacg cccagcagct ggctttgctc
    32761 acgtttacca tcaagcattt ctcgtgacat taacaccgca aattcatcgc cttttttcag
    32821 tttgcggaaa tccatttgcc actgcatggc tttaatcact gcgctcactt cggcgctggt
    32881 taaaccggcg tttctggcgc tggcaacaaa gcttcccccg acggtacctt tcagcagatt
    32941 gttgacccac tctccttgct gcatttcgct ggtcatttta aaaccgttag cggcagtacg
    33001 gtcataggtt cgggtttcac gacgagacac ttcccaggtg aggcgctgca gttcgccgtc
    33061 cgcggttaat gtccaggaga gttgttgacc gattttcagg ttacgcaatt ctttatcggc
    33121 agcagccagt tgggtgatat cacccatatc aataccatac tgattgagaa tgctgcttag
    33181 cgtatcgcca gtggaaacaa catattcatg cacgcccgct tcaccggcga ttttgtcatc
    33241 cagttcgtcc tggggaatgg cttcatcttc ttgtgcagct tgatcaatcg gctcactggc
    33301 ttcaggtaag agcgaacgaa tttcgttctg ttccagctca atggttttga caattggcgt
    33361 ggcatcgcgg tgataaacat agggccgcca gacagcgacg gccagagtaa gaacggtgag
    33421 cgaccccaac ataacgcggt gtggtcgcgg taaattatta aacgccaggg cgacagagcg
    33481 ggctatctgt tgcacgtaat cacttcctca ttaatctcct ttcaggcagc tcgcatactg
    33541 gttggctaat tgattcagga attctgaata gcttgtttta cccagtttga tattcgtccc
    33601 caggggatcc aacgttccca tacgaacgga tgtccctcgt gcgacgctct caacgaccgc
    33661 tggcctgaac tgtggctcag caaaaacgca ggttgctttt tgctcaacca actgtgttct
    33721 tatttcatgt aaacgctgcg cgccaggttg aatctcaggg ttaacggtaa aatgaccaag
    33781 cggtgtcagt ccgaactgtt tttcgaagta gccgtaagca tcgtgaaaaa cgaaataacc
    33841 tttccccttg agcggcgcga gctcgttacc aacctgcgtt tcggttgagg ctaattgtgc
    33901 ctcaaaatcc ttcaggttgg cgtcaagttt ggctcgactt tgcggcataa gttccactaa
    33961 ttttccatgg attgcaaccg ctgtagcccg cgctatctct ggggaaagcc aaagatgcat
    34021 gttgaaatcg ccgtgatggt gatcttcgtc acttttttcc gcgtgatcgt gatcatcatc
    34081 atcgtcgtga atacttttca tcagcagcgg tttcacatct tctagctgcg caatcgttac
    34141 ctgtttcgct tctggtaatt tacttaccgg tttttgcatg aacgcttcca tctccgggcc
    34201 aacccaaacg actaagtccg cgttctgtaa gcgttttaca tccgatggac gcagtgaata
    34261 atcatgttct gaagcgccgt caggcagtaa aacctgcgtt tctgttaccc catcagcaat
    34321 ggcagaagcg atgaacccaa cgggtttaag cgaagcgaca acggcggcat ctgcggcctg
    34381 tgttgcaccg ccccagagag cggcggataa tgctgcgaaa agaagcgttt ttttatgtaa
    34441 cataatgcga ccaatcatcg taatgaatat gagaactgtg atattataac atttcataac
    34501 tactgcaaga ctaaaattaa catgacaagt ctggtttccc tggaaaatgt ctcggtttct
    34561 tttggccaac gccgcgtcct ctctgatgtg tcgctggaac ttaaacctgg aaaaattttg
    34621 actttacttg ggccaaacgg cgcaggtaag tcgacactgg tacgggtagt gctcgggctg
    34681 gtaacacccg atgaaggggt tatcaagcgc aacggaaaac tgcgcatcgg ctatgtaccg
    34741 cagaagctgt atctcgacac cacgttgccg ctgaccgtaa accgtttttt acgcttacgc
    34801 cctggcacac ataaagaaga tattttgcct gcactgaaac gtgtccaggc cgggcatctg
    34861 attaacgcac cgatgcaaaa gctctcgggt ggcgaaacgc agcgtgtact gttagcgcga
    34921 gcattgttaa gtcgaccgca attattagtg ctggatgaac ccactcaggg cgtggatgtg
    34981 aatggtcagg tggcgttata tgaccttatt gaccaactgc gtcgcgaact ggattgtggc
    35041 gttttaatgg tatctcacga tctgcatctg gtaatggcaa aaaccgatga agtgctttgc
    35101 ctgaatcacc acatttgttg ttccggcaca ccggaagttg tttccctgca tccggagttt
    35161 atttctatgt ttggtcctcg tggtgctgaa caactgggta tctatcgcca tcatcataat
    35221 catcgtcacg atttacaggg acgaattgtt ttgcgtcggg gaaatgatcg ctcatgattg
    35281 aattattatt tcccggttgg ttagccggga tcatgctcgc ctgtgccgcg ggtccgctgg
    35341 gttcgtttgt agtctggcgt cgtatgtctt atttcggtga tacgctggct catgcctcat
    35401 tacttggcgt cgcgtttggt ttgttgctgg acgtgaatcc attctatgcg gtgattgcag
    35461 ttacgctgct gctggcgggc ggtctggtat ggctggagaa gcgtccacag ctggcgatcg
    35521 acacgttatt agggattatg gcgcacagtg ccctgtcgct gggcctggtg gtcgttagtc
    35581 tgatgtctaa tattcgtgtt gatttgatgg cttacctgtt cggtgattta ctggcagtga
    35641 cgccagaaga tctcatctct attgcgattg gcgtggtcat cgtggtggct attttgttct
    35701 ggcaatggcg caatttgctg tcgatgacga ttagcccgga tctggcgttt gttgatggtg
    35761 tgaaattaca gcgcgtgaaa ttgttgttga tgctggtgac ggcattgacg attggtgtag
    35821 cgatgaaatt tgtcggcgcg ttgattatta cttcactgct gattattcct gctgctactg
    35881 cacgtcgctt tgcccgcacg ccggaacaga tggctggtgt cgctgttttg gtggggatgg
    35941 tggcagtgac tggcggttta accttttccg cattttacga tacacctgca ggcccgtcgg
    36001 tggtgctatg cgcggcactg ttatttattc tcagtatgat gaaaaaacag gccagctaat
    36061 ctttcgctga acatatttgt cggatgcggc gcgagcgcct tatccgacct acggttcgct
    36121 atctctggta ggcctgataa gacgaacagc gtcgcatcag gcacactgcc agtgtcggat
    36181 gcggcgcgag cgaccaatcc gacttacggc atttctggcg gcgttatgcc aaagtgattc
    36241 cacgcccgcg tcgtcgccat acgcccacgc ggtgtacgct gcaaaaagcc ttgctgaatc
    36301 aaataaggtt ccagtacatc ctcaatggtt tcacgttctt cgccaatggc tgccgccagg
    36361 ttatccagac ctaccggccc accaaagaac ttatcgatta ccgccagcaa caatttgcgg
    36421 tccatataat cgaaaccttc agcatcgaca ttcaacatat ccagcgcctg agcagcgata
    36481 tctgccgaga tggtgccatc gtgcttcact tcggcgaaat cacgcactcg acgcagcaga
    36541 cggttggcaa tacgtggcgt accgcgcgca cgacgagcaa cttccagcgc gccgtcatca
    36601 ctcatctcaa gccccataaa gcgtgcgctg cgactgacga tatattgcag atccggcacc
    36661 tgataaaact ccagacgttg cacaatacca aaacgatcgc gcaacggtga tgtcagcgaa
    36721 cctgcgcgcg tggttgcacc aatcagggta aacggcggca aatcaatttt aatggagcgt
    36781 gccgccggac cttcaccaat catgatatcc agttggtaat cttccattgc cggatacaac
    36841 acctcttcca ccactggtga aagacggtgg atctcatcaa taaacagtac atcgtgtggt
    36901 tcaaggttag tgagcattgc tgccagatcg cccgcctttt ccagcaccgg accagaagtc
    36961 gtgcgtaaat taacgcccat ttcattggcg acaatattgg caagcgtagt tttacccaac
    37021 cccggaggac caaaaatcaa tagatgatcg agggcatcgc cgcgcagttt cgctgctttg
    37081 atgaaaatct ccatctgcga acgaacctgc ggctgaccaa catactcttc cagtaattta
    37141 gggcgaatgg cgcgatctgc cacatcttcc ggcaaagtgg taccggcaga aatcagacgg
    37201 tctgcttcaa tcatccttta cctcataacg cagcgcgtag ggcttcgcga attaatgttt
    37261 cactgctggc gtcagggcga gcgattttgc tcaccatgcg gcttgcttct tgtggtttat
    37321 agcccagtgc caccagcgca gcaaccgctt cctgttcagc atcgtcggtc gccgggctgg
    37381 caggagacgt gagtaccagg tcggcggctg gcgtaaagag atcgccatgc aaacctttaa
    37441 atcggtcttt catttcgaca atcaagcgtt cggcggtttt tttgccaata cccggcagtt
    37501 tcaccagtgc ccccacttct tcacgctcaa cggcattaac gaactgctgc gctgacattc
    37561 cggagaggat cgccagcgcc aacttcgggc cgacgccgtt ggttttgatc aactctttga
    37621 acaacgtgcg ctcttgttta ttgttaaaac cgtacagcag ttgcgcgtct tcacgcacca
    37681 caaagtgggt gaaaacgatc gcttcctgac ccgcttcagg gagttcataa aaacaggtca
    37741 tcggcatatg cacttcatag cctacgccgc ccacttcaat taacaccagc gggggttgtt
    37801 tttcaatgat gatgcctctg agtctgccta tcacatgacg ctcctgcgta atgaatcaaa
    37861 gataatgctg tatgataaaa aaatgctgga tagatatcca gcgaaggatg aagaaaactt
    37921 gcgaggtgtc tcgatgatct gaaaaatggc gcag
//
LOCUS       DAZKHK010000034        37601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKHK010000034 DAZKHK010000000
VERSION     DAZKHK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.034"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            226..1599
                     /gene="aroP"
                     /locus_tag="ACHOS5_001766"
     CDS             226..1599
                     /gene="aroP"
                     /locus_tag="ACHOS5_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP9776588.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYRYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1642..2496)
                     /gene="ampE"
                     /locus_tag="ACHOS5_001767"
     CDS             complement(1642..2496)
                     /gene="ampE"
                     /locus_tag="ACHOS5_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP9776589.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDGVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2493..3044)
                     /gene="ampD"
                     /locus_tag="ACHOS5_001768"
     CDS             complement(2493..3044)
                     /gene="ampD"
                     /locus_tag="ACHOS5_001768"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP9776590.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3132..4025
                     /gene="nadC"
                     /locus_tag="ACHOS5_001769"
     CDS             3132..4025
                     /gene="nadC"
                     /locus_tag="ACHOS5_001769"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP9776591.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4229..4669
                     /gene="ppdD"
                     /locus_tag="ACHOS5_001770"
     CDS             4229..4669
                     /gene="ppdD"
                     /locus_tag="ACHOS5_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP9776592.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4679..6064
                     /gene="gspE"
                     /locus_tag="ACHOS5_001771"
     CDS             4679..6064
                     /gene="gspE"
                     /locus_tag="ACHOS5_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP9776593.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNMDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQMLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6054..7256
                     /gene="hofC"
                     /locus_tag="ACHOS5_001772"
     CDS             6054..7256
                     /gene="hofC"
                     /locus_tag="ACHOS5_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP9776594.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7291..8334)
                     /gene="guaC"
                     /locus_tag="ACHOS5_001773"
     CDS             complement(7291..8334)
                     /gene="guaC"
                     /locus_tag="ACHOS5_001773"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9776595.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8490..8534)
                     /locus_tag="ACHOS5_001774"
     CDS             complement(8490..8534)
                     /locus_tag="ACHOS5_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776596.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8559..9179
                     /gene="coaE"
                     /locus_tag="ACHOS5_001775"
     CDS             8559..9179
                     /gene="coaE"
                     /locus_tag="ACHOS5_001775"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9776597.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQR
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9179..9922
                     /gene="zapD"
                     /locus_tag="ACHOS5_001776"
     CDS             9179..9922
                     /gene="zapD"
                     /locus_tag="ACHOS5_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP9776598.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            9932..10129
                     /gene="yacG"
                     /locus_tag="ACHOS5_001777"
     CDS             9932..10129
                     /gene="yacG"
                     /locus_tag="ACHOS5_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP9776599.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10217..10615)
                     /gene="mutT"
                     /locus_tag="ACHOS5_001778"
     CDS             complement(10217..10615)
                     /gene="mutT"
                     /locus_tag="ACHOS5_001778"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP9776600.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYAG"
     gene            complement(10675..13380)
                     /gene="secA"
                     /locus_tag="ACHOS5_001779"
     CDS             complement(10675..13380)
                     /gene="secA"
                     /locus_tag="ACHOS5_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9776601.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13442..13954)
                     /gene="secM"
                     /locus_tag="ACHOS5_001780"
     CDS             complement(13442..13954)
                     /gene="secM"
                     /locus_tag="ACHOS5_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP9776602.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14185..15102)
                     /gene="lpxC"
                     /locus_tag="ACHOS5_001781"
     CDS             complement(14185..15102)
                     /gene="lpxC"
                     /locus_tag="ACHOS5_001781"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP9776603.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15203..16354)
                     /gene="ftsZ"
                     /locus_tag="ACHOS5_001782"
     CDS             complement(15203..16354)
                     /gene="ftsZ"
                     /locus_tag="ACHOS5_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9776604.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16415..17677)
                     /gene="ftsA"
                     /locus_tag="ACHOS5_001783"
     CDS             complement(16415..17677)
                     /gene="ftsA"
                     /locus_tag="ACHOS5_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9776605.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17674..18504)
                     /gene="ftsQ"
                     /locus_tag="ACHOS5_001784"
     CDS             complement(17674..18504)
                     /gene="ftsQ"
                     /locus_tag="ACHOS5_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP9776606.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPGESTQQQNQAQAEQQ
                     "
     gene            complement(18506..19426)
                     /gene="ddlB"
                     /locus_tag="ACHOS5_001785"
     CDS             complement(18506..19426)
                     /gene="ddlB"
                     /locus_tag="ACHOS5_001785"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9776607.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDQQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQNALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEALQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19419..20894)
                     /gene="murC"
                     /locus_tag="ACHOS5_001786"
     CDS             complement(19419..20894)
                     /gene="murC"
                     /locus_tag="ACHOS5_001786"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9776608.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20948..22015)
                     /gene="murG"
                     /locus_tag="ACHOS5_001787"
     CDS             complement(20948..22015)
                     /gene="murG"
                     /locus_tag="ACHOS5_001787"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9776609.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22012..23256)
                     /gene="ftsW"
                     /locus_tag="ACHOS5_001788"
     CDS             complement(22012..23256)
                     /gene="ftsW"
                     /locus_tag="ACHOS5_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP9776610.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23256..24572)
                     /gene="murD"
                     /locus_tag="ACHOS5_001789"
     CDS             complement(23256..24572)
                     /gene="murD"
                     /locus_tag="ACHOS5_001789"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9776611.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24575..25657)
                     /gene="mraY"
                     /locus_tag="ACHOS5_001790"
     CDS             complement(24575..25657)
                     /gene="mraY"
                     /locus_tag="ACHOS5_001790"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9776612.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25651..27009)
                     /gene="murF"
                     /locus_tag="ACHOS5_001791"
     CDS             complement(25651..27009)
                     /gene="murF"
                     /locus_tag="ACHOS5_001791"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9776613.1"
                     /translation="MISVTLSQLTDILNGELQGGDITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNVANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27006..28493)
                     /gene="murE"
                     /locus_tag="ACHOS5_001792"
     CDS             complement(27006..28493)
                     /gene="murE"
                     /locus_tag="ACHOS5_001792"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP9776614.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28480..30246)
                     /gene="ftsI"
                     /locus_tag="ACHOS5_001793"
     CDS             complement(28480..30246)
                     /gene="ftsI"
                     /locus_tag="ACHOS5_001793"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP9776615.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30262..30627)
                     /gene="ftsL"
                     /locus_tag="ACHOS5_001794"
     CDS             complement(30262..30627)
                     /gene="ftsL"
                     /locus_tag="ACHOS5_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9776616.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30624..31565)
                     /gene="rsmH"
                     /locus_tag="ACHOS5_001795"
     CDS             complement(30624..31565)
                     /gene="rsmH"
                     /locus_tag="ACHOS5_001795"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9776617.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31567..32025)
                     /gene="mraZ"
                     /locus_tag="ACHOS5_001796"
     CDS             complement(31567..32025)
                     /gene="mraZ"
                     /locus_tag="ACHOS5_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9776618.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREPLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32627..33631)
                     /gene="cra"
                     /locus_tag="ACHOS5_001797"
     CDS             complement(32627..33631)
                     /gene="cra"
                     /locus_tag="ACHOS5_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP9776619.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33713..33799)
                     /locus_tag="ACHOS5_001798"
     CDS             complement(33713..33799)
                     /locus_tag="ACHOS5_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP9776620.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33811..34302)
                     /gene="ilvN"
                     /locus_tag="ACHOS5_001799"
     CDS             complement(33811..34302)
                     /gene="ilvN"
                     /locus_tag="ACHOS5_001799"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9776621.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34305..36029)
                     /gene="ilvI"
                     /locus_tag="ACHOS5_001800"
     CDS             complement(34305..36029)
                     /gene="ilvI"
                     /locus_tag="ACHOS5_001800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP9776622.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKYSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36148..36186
                     /locus_tag="ACHOS5_001801"
                     /pseudo
     CDS             36148..36186
                     /locus_tag="ACHOS5_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36347..37291)
                     /gene="leuO"
                     /locus_tag="ACHOS5_001802"
     CDS             complement(36347..37291)
                     /gene="leuO"
                     /locus_tag="ACHOS5_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP9776623.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9113 a        10457 c         9133 g         8898 t
ORIGIN      
        1 tttgaggctg acctttacaa acggtttctt tttaagcaac tcatcttcaa ccatgcataa
       61 agcgggtgca ttcgctgccg cttaccatta ttcttgatct gacggaagtc cttttgtaac
      121 aattcaaact tccttgatgt aaacaaatca atacaacaaa cggaattgca aacttacaca
      181 cgcatcactg cgtagatcaa aaaaacaatc accgcacgag gtttcatgat ggaaggtcaa
      241 cagcacggcg agcagctaaa gcgcggcctt aaaaaccgcc atattcagct tatcgcgctg
      301 ggtggcgcga tagggacagg gctattcctg ggtagtgcct ccgtaataca gtccgcaggg
      361 ccagggatta tcctgggtta cgccattgct ggttttatcg cctttctgat catgcgtcag
      421 ctaggtgaaa tggtggtcga agaacctgtc gcaggctcct ttagccactt tgcttataga
      481 tactggggca gttttgccgg tttcgcctct ggctggaact actgggtact gtacgtttta
      541 gttgccatgg cagagctgac tgccgtgggt aaatacattc agttctggta tccggaaatc
      601 ccaacctggg tttctgccgc cgtgttcttt gtggtgatca acgccatcaa cctgacaaac
      661 gtaaaagtgt ttggcgagat ggagttctgg tttgccatta tcaaagttat cgcggtggta
      721 gcgatgatca tcttcggcgc ctggctgcta ttcagcggca acggcggccc gcaggcgagc
      781 gttagcaacc tgtgggatca gggtggtttc ctgccacacg gcttcaccgg tctggtgatg
      841 atgatggcga ttatcatgtt ctctttcggt ggtctggaac tggtggggat caccgcagca
      901 gaagctgata acccggagca aagtatccca aaagcaacta accaggttat ctaccgcatc
      961 ctgattttct atattggttc gttagccgtt ctgctctcac taatgccgtg gacccgcgtt
     1021 accgccgata ccagtccgtt tgtgctgatc ttccacgagt taggcgatac ctttgtagcg
     1081 aatgcgctga atatcgtggt actgactgct gcgctctccg tgtacaacag ctgcgtatat
     1141 tgcaacagcc gtatgctgtt cggtctggca caacaaggga acgcgccaaa agcgctggcg
     1201 tctgtcgata aacgcggcgt accggttaac actattctgg tgtctgcgct ggttacggca
     1261 ttgtgcgtac tgattaacta ccttgcccca gagtccgctt tcggactgtt aatggcgctg
     1321 gtggtatctg cactggtcat caactgggcg atgatcagtc tggcgcatat gaaattccgt
     1381 cgtgccaaac aagaacaagg cgtggtaact cgcttccctg ccctgcttta tccgctgggt
     1441 aactggatct gcctgctgtt tatggcggcg gtactggtga ttatgctgat gaccccagga
     1501 atggcgattt cggtatacct gatcccggta tggctgatcg tgttaggtat cggctatctg
     1561 tttaaagaga aaaccgccaa agccgtaaaa gcgcattaat ctctctacgc cctcacccgt
     1621 gcagggtgag ggcaataatc tttacaccaa cgccccgtaa atcgtcagta gcgcaattac
     1681 caccacgacc acgaacgagg ttttcttcgc cattgaaacc gctgcctttg gcgtctccac
     1741 cttatcgaca tgcggttcac gcgccagaga gaactgtgcc agacgcgtta atacctgata
     1801 ttgcgaagta tggaaatcac ccagcgaagc aaaccaggcc ggtaacgctt tctcaccatg
     1861 accgatcaag gcatatacca cacccgcaag acgaaccggc acccaatcca gtacatgaag
     1921 cacgccatca atgccggact gtaaacgatg atgcggcgtc tggtatcgcg ccagccagta
     1981 ttgccatgca cgcaaaaacg cataccccat cagcgtaacg ggtccccagg ttccccccac
     2041 aatcagccag aacagcggag caagataaaa acgaaagtta atccacagca atgcattttg
     2101 cagctcacgc aaatactcac gttcatcgca gcctgccggg acgccgtgaa tcatggtgag
     2161 ttcgccagcc atcgtggcac gggcatggct atcattacgt gaagcagctg tcagataagc
     2221 atgataatga agacgaactt tacctgcgcc aatacacagc aaaccaatca gcagccacac
     2281 cagcagcgtg ggaacgttga acaatactcc ctgcaatgcg cgtaacagta aaaaagtcac
     2341 gcccatcgca ataatggtca tgcctaatgt gcgcccgaga gaaaaatgtt tcacccgccg
     2401 aaagaacgct tcaagacgat gatcaagctg ccagtgctcg cccaacttaa acaggcgctc
     2461 gaaaattaac accagtaagg ttgtaaatag cgtcatgttg tctccttgct gaccagtgca
     2521 cgaaaccgtg cccaatcaaa tgcagggccg ggatcggttt tccgatccgg cgcaatatca
     2581 caatggcccg tcatgttatt agcgatatct ggatagcgat caatcagtgc ccgcgtaacc
     2641 gccgcaagct gttgatactg cgcatcggta tacgccagcg tatcggtgcc ttccagctca
     2701 atcccaatag aaaaatcatt gcagcgttca cgcccctgat actgagagac tcccgcatgc
     2761 caggcacgtt tatcgaaagg aacatactgg actatttcac catcacggcg aatcaaacag
     2821 tgagcggaga cgcgcaaatg ggcgatctca gcaaagaaag gatgtgcctg cggatcaata
     2881 gttccagtga ataatgcgtc gatccacgga ccgccaaact cgcctggcgg caggctaata
     2941 ttgtgcacca ccagcagggt gggtgtttcg tcatccgggc ggcaatcgta atgtggtgag
     3001 ggaacgcggc gcgcgccaac cagccacccc tgttctaaca acatgcagga tctccttata
     3061 tgtggtgcta atacccggtt cagagtagca tgtttctacc ttatgattcg ttagctatct
     3121 ggagttttaa catgccgcct cgccgctata accctgacac ccgacgtgac gagctgctgg
     3181 aacgcattaa tctcgatatc cccggcgcgg tggcccaggc gctacgggaa gatttaggcg
     3241 gaacagtcga tgccaacaat gacattacgg caaaactttt accggaaaac tctcgctctc
     3301 atgccacagt gatcacccgc gagaatggcg tattttgcgg caaacgctgg gttgaagagg
     3361 tgtttattca actggcaggc gacgatgtca ccattatctg gcatgtggat gacggcgatg
     3421 tcatcaacgc caatcaaccc ttgttcgaac ttgaaggccc atctcgcgtg ctgttaacgg
     3481 gcgaacgcac tgcgcttaat tttgtgcaaa ctctttcagg agttgccagt aaggtacgcc
     3541 actatgtcga attactggaa ggcaccaaca ctcagttgct ggatacgcgc aaaaccttac
     3601 ccggcctgcg ttcagctctg aaatacgcgg tactttgcgg cggcggagcg aatcaccgtc
     3661 tggggctttc tgatgccttc ctgatcaaag aaaaccatat tattgcctcc ggctcagtgc
     3721 gccaggcggt cgaaaaagcg tcctggttgc acccggatgc gccagtagaa gtcgaagtag
     3781 agaatctgga agaacttgat gaagccctga aagcaggtgc cgatatcatc atgctggata
     3841 acttcgaaac agaacagatg cgcgaagccg tcaaacgcac caacggcaag gcgctactgg
     3901 aagtgtctgg caacgtcact gacaaaacac tgcgcgaatt tgccgaaacg ggcgtggact
     3961 ttatctccgt tggtgcgcta actaaacacg tacaggcact cgacctttca atgcgttttc
     4021 gctaattgct aacagattta tccgctccat cgcgggcgga taaatctccc cttccccaac
     4081 tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg
     4141 aagctgcttt cccgtttcgt tttttgcccc tcgcttgtgc tgatggcgtt tgccaaagta
     4201 gcgccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga
     4261 actgatggtg gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca
     4321 aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac
     4381 cgccgtagag ttgtgcgcgc tggaacatgg tggattaaat acctgcgacg gtggcagcaa
     4441 tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt
     4501 ggtgtcgctg accgggcaag aaagtctcaa tggactaagc gtcgtcatga cgccgggttg
     4561 ggataacgca aacggcgtca ccggctggac gcgtaactgc aatattcaaa gtgacagcgc
     4621 attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat
     4681 gaatattcca cagctcactg ccctgtgtct gcgttatcag ggagtcttgc tggatgccag
     4741 cgaagaggtg gttcatgttg cggtagtcga tgcaccttcg catgagctac tggacgcatt
     4801 gcatttcgct accaccaaac gtattgagat cacctgctgg acacgccaac aaatggaagg
     4861 tcacgccagt cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa
     4921 agcagagttg ctgactcgaa cgttacaatc tgcgctagaa caacgcgcgt ctgatattca
     4981 tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc
     5041 tttaccggac gtttcaccgg atgccggagt cgcattaacc gccagattaa aagtgctggg
     5101 aaacatggat attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc
     5161 aggaaacgcc gtctcatttc gtattgcgac cttagcatgt cggggtggtg aaaaggtggt
     5221 attaaggttg ttacagcagg tgaaccaggc actggatgtt aacacgcttg gaatgcagcc
     5281 gtcacaactg gtggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac
     5341 tggccctaca ggcagcggca aaacggtcac gctttatagt gccctgcaaa tgctgaatac
     5401 cgctgacatt aatatttgta gcgtcgaaga tccggttgag atccccatag ccggactaaa
     5461 ccagacgcaa atccattcgc gtgccggact cacctttcag ggcgttttgc gtgcgttatt
     5521 gcgccaggat cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cggcagaaat
     5581 tgccattaaa gccgcgcaaa ccggtcacct ggtgttgtct accctacaca ctaattccac
     5641 ctgcgaaacg ctggtacgtt tacagcaaat gggagtcgcc cgctggatgc tatcatcggc
     5701 gcttacgctg gtaatagccc agcgtctggt acgcaaactt tgcccgcatt gtcgccggca
     5761 gcaaggggag cccatccata ttccagacaa tgtatggcca tcgccgctgc cccactggca
     5821 ggcacccggt tgtgtacatt gctaccacgg tttttatggt cgcacggcct tatttgaagt
     5881 tctgcccata acaccggtca tacgtcagct tatttccgct aataccgacg ttgaatcgct
     5941 ggaaacgcac gcccgacagg cgggtatgcg aacgcttttt gaaaacggct gcctagccgt
     6001 ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta ttggggatgc cgcatggcga
     6061 gtaagcaact ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc
     6121 tatgggcaga gagccgtgct ttgctgctca tggcactaca gcaacagatg gttaccccac
     6181 taagcctgaa gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag
     6241 tcattcatca actggcgacg ctactcaaag ccgggttaac gctttctgaa gggctggctc
     6301 tgctggcgga acagcatccc agtaagcagt ggcaagcgtt gctgcaatca ctggcgcacg
     6361 atcttgaaca gggcgttgcc ttttccaatg ccttattacc ctggtcagag gtatttccgc
     6421 cgctctatca ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct
     6481 ttgaactggc gcgtcagcaa aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt
     6541 tacgttatcc cattatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt
     6601 ttgtactgcc ggagtttgcc gctatctata agacctttaa cactccgcta ccggcgctaa
     6661 cgcaggggat catgacgctg gcaagtttta gtggcgaatg gggttggttg ctggtgctat
     6721 tcggctttct gctggcgata gccaataagc tgctgatgcg ccacccaacc aggcttatcg
     6781 cacggcagaa attgctgtta cgcatcccga ttatgggttc actgatgcgg ggacaaaaac
     6841 tcacgcagat ctttacgatt ctgacactga cacaaagtgc aggcatttct tttttacagg
     6901 gcgttgagag cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa
     6961 tccagcacga tatcagtaac ggtcacccca tctggctggc gctaaaaaat gccggtgagt
     7021 ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggatctca
     7081 tgttagacaa cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg
     7141 cagccttact ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg
     7201 tggcaatgta tctgccaatt ttccatttag gcgatgcgat gagtggaatg ggataacgct
     7261 ggcgtgttgc tccacgccag cgttggtata ttacaggttg ttgaagatgc ggttttcttg
     7321 ttcctgcaca cggataaacg tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac
     7381 gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caaccgggcc
     7441 tcgcagcggc agcttaacgg ttttaccttc tgctgcgcga tattccgcaa cgccgccaac
     7501 gtgacgtttc atcgcagact ctgagctcat gccgtagaac aacataaatt tctcgccgtt
     7561 ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcattac
     7621 gaaatcggca ccgccgccaa aggctttcgc gacatcgccc ggcgtggtgc agccgccatc
     7681 gctgacgatc attccgccca gaccgtgcgc agcatcggcg cattcgatca ccgcagaaag
     7741 ttgcggataa ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc
     7801 aactttaacg atatcggcac cggagaggat aagctcctca cacatttcac cagtcactac
     7861 gttgcctgca caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac
     7921 gaagtgttcg gaataaccat tcgccacgtc aatacaaacg aaatttaatg ccgggttcag
     7981 gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac gtgccggtag aaaccatcac
     8041 atgtttcagc acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaata
     8101 gtgtttatgc acagcagtca gaatatcaaa agaagccagc gcagaggcca tagaaaatgt
     8161 gcctacggtg tccatatttg cggcaataat cggcacgccg gaccaactct gacctgaatg
     8221 tttgaaggtg aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt
     8281 agggcggatg agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg
     8341 attcctgggg ttaatggcta taagggtaag agcgcaactc cagtgacgtt atcatacgca
     8401 ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt
     8461 tacctggtaa atagtgagcg aacgtttcat caatttcgct ttcgtgcgct catttttaac
     8521 gttttgataa tcatgatgaa ttcccgggaa tataaattat gaggtatata gttgccttaa
     8581 cgggaggcat tggcagtggc aagagtaccg ttgccaatgc gtttgctgat ctcggaatta
     8641 acgtcattga tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac
     8701 atgccattgc tgatcacttt ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc
     8761 gggccttgcg cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgccctgc
     8821 tgcatccgct gattcagcaa gagacgcaac accagatcca gcgagcaacc tccccctatg
     8881 tactgtgggt tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc
     8941 tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gacgatgtaa
     9001 ctcgcgagca tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagct cgccttgccg
     9061 tggcagatga cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc
     9121 gcctgcacgc acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat
     9181 gcagacccag gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat
     9241 tgagtttttg attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct
     9301 gcatttcttc cgtaatgtca gcgaattact ggatgttttc gagcgcggcg aagtccgcac
     9361 tgagctgttg aaagaacttg accggcagca acgtaaactc cagacctgga ttggcgtgcc
     9421 cggcgtggac cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt
     9481 attaatctcc gcgccgcgta tcgggcaatt tctacgagaa gatcgtttga ttgctctggt
     9541 gcgtcagcga ctgagcatcc cgggcggctg ttgcagcttt gatttaccta cattgcacat
     9601 ttggctgcat ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct
     9661 gaacccgctt acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt
     9721 ccgtaaacaa accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct
     9781 gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa atttccggtc ataagagccg
     9841 ttttgccatt cgttttatgc cgctggacag tgaaaacgga caggtaccag aacgtctgga
     9901 tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa actattacgg tgaattgccc
     9961 aacctgcggg aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa
    10021 acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag
    10081 tggcgatctt tccgaaagcg atgactggag cgaagagcca aagcagtgac attctatctg
    10141 atgcgacgct ggcctgataa aacgcgccag cgtcgcatca ggcatcatat gcaaatgtcg
    10201 gatgcagcgt gacgccttat ccggcataca cacgcttaag tttcgcaatt accggttcat
    10261 tggctggcgg aaaatcatcg gcattaagac cgaccagcga catccactca ccgggttgcc
    10321 cttctttacc ccacggctcc ccttcccagc tttcgaccag ccagaaccac agagttatat
    10381 gcctgtccgg gaattcatat tccagttttt caaatagcga aaaatgttgt ggggtaatcc
    10441 cgacttcttc ctgaagttca cgcaccaccg cctgttccgg cgtttcgccc atttcaactt
    10501 taccgccggg aaactccagt ttattcgcca tgtgcgcatc tgctgcgcga cgcgttataa
    10561 agatttcatt gttctcgttg cgaataatac ctaccgcaat ttgcagcttt ttcattgtct
    10621 taaacctata aaaaaggcgc agaatcctgc gccttttact tcaactgtta gcttttattg
    10681 caggcggcca tggcactgct tgtatttttt accagaacca cacgggcaag gatcgttacg
    10741 tcctactttg cgttcaccgg tttgcgccgc cagtgcagct gcggctgcag agtcgtcatc
    10801 ctgatggcta agctgctgca tttgcgctaa acgctcggct tccatacgac gctgttgttc
    10861 cagctcctca acctcttcag gcatacgtac ctgaactttg ctcagcgtac tgataacttc
    10921 atatttcaac gactccagca tcgctgcaaa catggagaac gattcacgtt tgtattcctg
    10981 cttcggatct ttctgtgcat agccacgcag gtggataccc tgacgcagat agtccatcgc
    11041 tgccaggtgc tctttccaca gagagtcgag agtttgcagc atgacgcctt tctcgaagtg
    11101 acgcatcatc tcagcaccaa ccacttcttc tttacgctga tacacttcga tggactgcgc
    11161 cagaatgcgc tcacgcagcg tctcttcatg cagttctggt tctttatcca gccactcggc
    11221 aattggtaaa tcgaggtcga aatcgttctt cagacgttcc tgcagccccg gaatatccca
    11281 catttcttcc agcgactgtg gcggaatgta ggcatcaatg gtcgctttga acacatcttc
    11341 acgaatgctg ttgatggttt cgctcacatc gctgacatcc agcagttcgt tacgctggga
    11401 gtaaatggcg cgacgctgat cgttagccac gtcatcatat tccagcagtt gcttacgaat
    11461 gtcgaagtta cggctttcaa ctttacgctg ggcgttggca atcgctttgg tcacccacgg
    11521 gtgctcaatg gcttcgcctg gcttcatacc cagtttacgc atcatgccgg atactcggtc
    11581 ggaagcaaaa atacgcatca gcgcatcttc catcgacagg tagaaacggg aagaaccagc
    11641 atccccctga cgaccagaac gaccgcgcag ctggttatcg atacgacggg attcgtgacg
    11701 ttcagtaccg atgatatgca ggccacctgc tgccagtacc gcatcgtgac gtacctgcca
    11761 gtcggcttta attttttcaa tttgctctgc agtcggattt tccagcgcgg caacttctgc
    11821 ctgccagcta ccaccgagca caatatcggt accacgaccc gccatgttgg tcgcgatagt
    11881 caccgcagcc gggtaacctg cctgagcaac aatcgccgct tcgttggcat ggaatttggc
    11941 gttcaggacg ttgtgcttaa taccggcttt ggtcagttca tttgacacca gctccgattt
    12001 ttcgatggag attgtaccca ccagcaccgg ctggcctttc gcagtacgtt ctttgatatc
    12061 ttcaatgatc gcctgaattt tttccgcttc agtcatgtag accaggtccg gcaaatcttt
    12121 acgaatcatt ggacggttgg tcggaacaac gacagtatcc agcttataga tggagctgaa
    12181 ttcgaaagct tcggtatcag cagtaccagt catccccgcc agtttttcat acagacggaa
    12241 gtagttctgg aaggtgatcg aagccagcgt ttggttttcg ttctggatct gcacaccttc
    12301 tttcgcttcc acagcctggt gcagaccatc ggaccagcga cggccctgca tggtacgacc
    12361 ggtgtgttcg tcaacgatga taacttcacc atctttaacg atgtagtcga cgtcgcgggt
    12421 aaacagcgca tgagcgcgca gcgccgccgt tacgtggtgc atcagcatga tgttggccgg
    12481 agagtacaga gactcacctt catccatgat gccttcttta accagcagtt cttcaatcag
    12541 aaccagacca cgttcggtca ggttcacctg gcgagatttt tcatccaccg agaagtggcc
    12601 ttcgccctgg aaggtttcgg agtcttcttt ttcctgacgg atcaggtgcg gaataatttt
    12661 attcacgcgt ttatacattt ccgagctgtc ttctgctggg ccggaaatga tcagcggtgt
    12721 acgcgcttca tcgatgagga tggagtccac ttcgtccacc agcgcatagt gcagtttacg
    12781 ttgtacacgt tcttcagggc tgaatgccat gttgtcgcgc aggtagtcaa agccgtattc
    12841 gttgttcgta ccgtaagtga tgtcagcagc gtaggcttca cgctttgccg gtgctggcat
    12901 gcccggtagg ttgataccga cagtcaggcc aaggaattca aacagcggac ggttgttttc
    12961 ggcgtcacgt tgcgccaggt agtcgttgac ggtaactacg tgtacgcctt taccggttag
    13021 tgcgttcagg taagcaggca gcgttgcggt cagggtttta ccttcaccgg tacgcatttc
    13081 ggcgatgcag cgttcgttaa gaaccatacc gccgagcagc tgtacgtcga agtgacgcat
    13141 gccaaagacg cgcttacttg cctcacgcac cacggcgaaa gcttccggga tcagattttc
    13201 cagcacttcg cctttttcca gacgcgcacg aaactctgcg gttttccctt tcagttcttc
    13261 gtcggagagt ttttccatct ccggttccat ggcattgatg atgttgacca ctttgcgcat
    13321 ccggcgcagg gtgcgatcgt tacgactacc gaaaacttta gttaataatt tgattagcat
    13381 aataaaatct caaacgcccc gcgttgcgga gttaataaaa tgaagtaaag gtttattgtt
    13441 gttagctgag gcgttgaggg ccagcacgga tgccttgcgc ctggcttatc cagacgggcg
    13501 tgctgaattt tgcttgtggg gtaaaatgcg cataatcaat gcgataaccc ttttcagacg
    13561 gcgtgccttc ctgggtcaga agcgcgctaa gcgtatccag taatgcaaga tgttgcgcct
    13621 gaagaggcaa agattcttca gcaacgggca gcgtttgcgg tgccatcgcg aaggaaagat
    13681 ggcggattac cgtgcgaatg gcatgttgat gccagtaatc aacggaatag ttcgaattcg
    13741 ggcggcgcgt gttcgcttcc agcaaggcca attgaccaaa gttaactttg gctgaaggct
    13801 cgtggttgcg ggttgtcgct tttgcgggcg cgtttggttc ggcggcattg ctgagtgcag
    13861 gcaaacctaa actcgccgcg accatcccta ataagagatg cggccagaag tagcgtttac
    13921 caaactgtcg ccagcgcgtc agtattccac tcacgttatt gccatcccga tcgtattcaa
    13981 acgtatttag cgcacaaatc ttatcattaa agcctgaagt ccacagcacg aatgaagata
    14041 agttgtgcgt aaaagtgctt aagaaaacag cgttcgcacc gttttttata gctcttataa
    14101 aattcaaaaa aaaacgactg gttcacctgg ccggagagag tgccagattt gccagtcgaa
    14161 ttttatacga cagtataaat gtcgttatgc cagtacggct gaaggcgctt tgaaggccaa
    14221 cggcagttct gcgtcgtcct ggaaggtcac atattcccag gcttcctgtt ttgcaaggac
    14281 agcctgcagc agtttgttat tcagtgcatg accagattta taagcggtaa atgcaccaat
    14341 aatattgtga ccacacatga acaagtcacc gatcgcatca agcattttgt gacgcacaaa
    14401 ttcgtcttca aaacgcaggc cgtcttcgtt cagtacgcga taatcgtcaa caacgatggc
    14461 acaatcgaag ctgccgccca ggcacaaacc acgggactgc agatattcga tatcacgcat
    14521 gaaaccgaac gtacgcgcac ggctgatctg gcgcataaat gcatcagcgg agaagttcat
    14581 cgcatagcgc tggttgctgg aatcaatagc cggatggtta aaatcgatgg tgaaatccag
    14641 agaaaaacca ttgtacggct taaattcagc ccacttatcg ccatcttcga cacgaacagt
    14701 ttctttaatg cgaacaaatt tcttggcgca gttcaactcg tcgataccgg cgtcaagcag
    14761 caggtataca aacggagcgg cgctgccgtc catgatcggg atttccggcg cgttaacttc
    14821 gataacaatg ttatcgatgc ccaggcccgc gagagcagca ttgaggtgct ctacggttga
    14881 aatccgcaca tcatgctcgt tgaccagaca cgtacagagc atggtatcac gcacagattt
    14941 ggcatcggcc gggaaatcta ccggtggatt caagtcggtg cgacgataga tgaccccggt
    15001 gttggccggc gcagggcgta acgtcagggt gactttcttg ccggtatgta aaccgacacc
    15061 cgtcgcctga acgatacgtt taagtgtcct ttgtttgatc atcgtattat ctcgccaaat
    15121 tacctatcca accgaagtgt actatacatt cggcgggcca gtttagcaca aagagcctcg
    15181 aaacccaaat tccagtcaat tcttaatcag cttgcttacg caggaatgct gggatatcca
    15241 gataatccgg ctctttcgca gtttgcggcg cattgtcatt cacgacttta gcaaccggct
    15301 tctgctcctg ggtcagcgga gccatcccgt gctgctggta gcgatccatc actggctgct
    15361 gaacctgctt attggtcacc agagtgattt caggacgttt gtccatgccg atacctgtcg
    15421 caacaacagt tacgcgcagc tcgtcattca tatccgggtc aagagaagta ccgataacca
    15481 cggtcgcgtt gtcggaagca aatgcacgga tggtgttacc taccgtttcg aactcatcca
    15541 gacgcaggtc gaagcccgct gtgatgttta ccagtacgcc gcgtgcgcca gacaggtcga
    15601 tatcttccag cagcggagaa gagatagcca tttcagcagc ttcttccgca cggtcttcac
    15661 cgctcgccac gccagaaccc atcattgcgt agcccatttc agacattacg gtgcgtacgt
    15721 ctgcaaagtc cacgttcatc aaacccggac gagtaatcag ttcggcgata ccttgcacag
    15781 cgcctttcag tacatcgttc gctgcgccaa acgcatccag cagggagata ccacggccca
    15841 gaactttcag cagtttgtcg ttcgggatag tgatcagaga gtccacatgc ttggacagtt
    15901 cagtgatccc ctgctccgcg aatgccatac gcttcttgcc ttcaaagttg aaaggcttag
    15961 tgacgacagc aacggtcagg atacccaaat cttttgccac ttcagcgacg actggtgctg
    16021 cacctgtacc ggtaccacca cccatacccg cagcaataaa gaccatgtct gcaccttcca
    16081 gcgccgcacg cagtgcatcg cgatcctcat cagccgcatt gcggccaact tctggattag
    16141 cgccagctcc cagtcctttg gtgataccgc taccgatttg aatcgtctgt ccaaccgctg
    16201 ttttacgcag cgcttgtgca tcggtattta ccgcgaagaa ttcaacacct tcaatgcgct
    16261 cacgcaccat gtgttcaaca gcattaccgc cgccgccgcc gacgccgatg actttaatca
    16321 ccgcgtcatt ggtaagttcc attggttcaa acatagtttc tctccgattt gtgcctgtcg
    16381 cctgaggccg taatcatcgt cggcctcata aaaattaaaa ctcttttcgc agccaactat
    16441 tgagtcgctt gatccacgag ccaactgatg ctgtaacacg tttttctact tcagcttcac
    16501 cgttaagatg tgactctttc ccatagtgaa gcaatcccac cgccgtcgaa taatacggct
    16561 cctgagcata atccgttaaa ccggtaatgt tcagcggcgc gccgatacgc acttgcgtat
    16621 gaaacacgcg ctgagcacag gctgcaagac cttcgatctg cgctgcgcca ccggttaata
    16681 caatgcctgc cgccaggtga tgtttaaccc cttgttggcg aagcttttcc tgcaactgca
    16741 atatctcttc gttgaccaga ttgagcagct cggtatagcg cggctcgatc acctctgcca
    16801 gggtctgacg ttgtagactc cgcggcggac gaccgcctac gctcggcact tccacgctct
    16861 catcttttcc aacgatggag cccagcgcac aaccgtggcg aactttaatc gcttcggcgt
    16921 cacttggcgg tgtgccaaag gcgtaagcga tatcactggt cacgacattg ccagcataag
    16981 gaattacctt cgtgtggcgc aatgccccac cggtataaac ggcgatatcc attgtaccac
    17041 caccgatatc gacgacgcag acacccagtt cacgttcatc ttccgtcaat accgaataac
    17101 ttgatgccag tccggcaaat atcagttggt caactttcag cccacaacgt tcaaccgctt
    17161 tgacgatgtt tttcgccata tcgttgtgac atgtgatcag gtgcactttt gcctgcatcc
    17221 gcacgcccga aagccctacc ggattcttga tcccttcctg atagtcaatc gcatactctt
    17281 gcgggatcac atgcagcaca cgatgctcat cgcgcacacg caccgatttc gcggtatgga
    17341 cgacgttttc cacatcttct tgcgtcactt cttcttcaga aataggcacc ataccaattt
    17401 cattctggca gctgatgtgc ttaccagaaa gcgccagata taccgaagag atctgacaat
    17461 ctgccatcaa ttctgcctgg tcaatggcgc gttgtacgca cttgaccacg gattcgaggt
    17521 cgttcacccc gcctttatcc ataccacgcg acgggcagct gcccacgcca atgatattga
    17581 ccataccgtc gggcagaact tcccctacta aagcggcaac cttcgcggta ccaatctcca
    17641 gtcctactac cagttttctg tccgtcgcct tgatcattgt tgttctgcct gtgcctgatt
    17701 ttgttgctga gtagattccc ctggcggcaa gggcgcccag cctactgccg ctccagagtc
    17761 ataacgcaaa tcaacgtagc taatccgttt gccatcggtt tgcgcctgct gctgtaaaac
    17821 cggataaagt tctacaaagc gagccaaacg tttcatcgta tcgccccggc caagattgag
    17881 cttaatatcg ttattcagcg tcaactgcca ggaacgtcgc gcggtcatcg ccgcttcctt
    17941 cagagtaaat ctgtcctttg ccagcatctg ccccatttcg cgatagccct gcaacacttc
    18001 attggcgctg ccttccgggc catacagcat tggaagcacc tgcttgctgg tgcgatctgg
    18061 cggcacgctg aaggtgtttc cttccgcgtc taccatatgt tgatcattcc accgcgcaat
    18121 cggcacatat tcaaccagat gaatcttcaa ttcatcaggc cactgctttc tgacgctcac
    18181 ctgcttaatc cacggcaggc gttgttctat ttgcgtctgg atgatgttga catcctgggt
    18241 cataaaggta cctggctcac ccaatgccag gatcgactgc cggatatcgt cattacgcgt
    18301 gtaatggcgt tcaccggtca acaccagctt tgagagcggc aggcgttgcg catcttccat
    18361 ccagcccaac acgacccagc cgctcaccaa cactgtcgtt aaaacggtca gcaggaaaag
    18421 gatccccgcc agacgcgttc cattattgcg gcgagaagaa acctcttctt cgctatttcg
    18481 cgtgttcaga gcagcctgcg acatattagt ccgccagttc cagaattcgt actaccaact
    18541 gcgagaagct catacctgcc tgacgtgccg ccatcggcac cagactgtgg ctggtcatac
    18601 ccggtgaggt attggcttcc agcagataaa actgtccatc actgtccagc ataacgtcaa
    18661 tacgtcccca tcctttgcaa cctaacgtcg tccatgcttt tagcactaat gcctgcaaat
    18721 tggcctcttg taacgcttcc agacctgcgg ggcagaaata ctgtgtctca tcagagagat
    18781 acttcgcctc ataatcatag aaggttccgg ccggttgaat acgtattgac ggtaaaattt
    18841 cttcaccgag tatcgcaacc gtgaactccg gcccacttag ccatttttca atcaatactt
    18901 cttcatcgtg ctgaaatgcc aatcttaatg cattttgtag agcattttct gctactactt
    18961 ttgacattcc cacactggaa ccttcgcggc tcggcttaac gataaccggc aaacccagag
    19021 aagaaatttc tgctaactgc tgatcgttaa ggcctttttc aaactctgcg cgggttaacg
    19081 ctacccacgg cgcgaccggt aaaccggcac cctgccacag aagtttgctg cgtagtttat
    19141 ccattgaaag cgcagatgcc atcactccgc tgccggtata aggcaagccc atcagctcga
    19201 gcattccctg cagcgtacca tcttcaccgc cgcgaccgtg tagcgcgata aacactttct
    19261 gaaagcccat cgacttcagt tgcgtcacgt cgacttcttt cgggtcgaca ggatacgcgt
    19321 caataccgcc ttcacgcagc ccggctaaca ccgctgcacc agaattcaga gaaacttccc
    19381 gctcagcgga ggtcccgccc aacaggaccg cgattttatc agtcatgttg ttcttcctcc
    19441 ggagtttgcg gcttcagttt gatttcagct aaagaacggg caatttttcc gatattacca
    19501 gccccctgaa cgagaatcag gtcgttaccg gttaataccg gtgccagcat ctcggctacc
    19561 tgcgccgggt ccggcaccag aatgggatca attttcccac gtccacgaat tgtgcgacac
    19621 agcgaacggc tgtccgctcc cggaattggc gcttcgccag ccggatacac ttccagcatc
    19681 aacagagtat caacctgcgt cagcacattg gcgaaatcat catacaggtc gcgcgtacgg
    19741 gtaaaacggt gcggctgaaa cagcattacc aggtttttat ccggccagcc tgcgcgcgcc
    19801 gctttaatag tggcgtccac ttccgtcggg tggtggccgt agtcatcgac cagcattgcc
    19861 gtaccgcttt ttccattcac tggctctagc gggaattcac cgaggaaatc gaaacggcgc
    19921 cccgtcccct ggaagctttc cagcgcacgc aaaatggcct cgtcgtcaat tccctcttcc
    19981 gtagcaaccg caaccgcagc tgcggcgttc agcgcgttat gacgacctgg cgcattcagg
    20041 gtgacgcgca tcggctcttt gtcctggcgc agcagcgtaa agtgcccctg cgggccaatc
    20101 tgctgataat cttctacacg cacgtcggca tcttcgctga agccgtaagt cgtggtctga
    20161 cgtcccacac gcggtaacaa ttcgcggatc accggatcat caacacacat caccgcacga
    20221 ccgtaaaacg gcaggttgtg cagaaaatta ataaaagtct gttttaaatt ctcaaagtcg
    20281 ccctggtagg tatccatgtg gtcggcttcg atattggtga caatcgccac catcggttgc
    20341 agatgcagga acgatgcatc actctcatct gcttcggcaa ttaggtaccg accatgcccc
    20401 aaacgcgcat gaacgcccgc cgcttttacc agcccaccgt taacgaaggt tgggtcgagt
    20461 cccgcttctg cgtagatgct ggaaaccatc gcagtggttg tcgttttgcc gtgcgttccg
    20521 gcaatggcga tgccatgacg aaaacgcatt aactcagcca gcatttcggc acgacggatc
    20581 accggaatac gcgcttcatg agctgcgaca atttccgggt tatcggcaga aatcgcgctg
    20641 gaaacaacga ccacgctggc atcacgtacg ttttccgggc gatggttgaa ataaatcgtc
    20701 gccccaagat tcattaactg ctgcgtgacc ggatttggcg ctaaatcgga accactgatc
    20761 tgataacctt cattggccag aacttcggca ataccgccca taccggcacc accgatgccg
    20821 acaaaatgta tgtgccgaac gcgacgcatt tcgggcacga tggaacgcag ttttgccaat
    20881 tgttgtgtat tcattcttta cgccattaac ttcttaaatt catacgatgc aaaaggcatc
    20941 gctacaatta cgcccgggca gcccggctca cttcatttgc cactcgctcg gtggcatccg
    21001 gaatggatgc agcgcgggcg cgttctgcca tggttaataa ggtttctcgc gaccacccgg
    21061 ccagggtgtt ggcgacagca tccacgctaa gctgtgactg ctcgataatt ttggctgcgc
    21121 ccgctttttc cagcggtagc gcattccagt attgctgacg gtctttatgt tgaaacggca
    21181 caaacaacgc cggtagtcct gccgcggcga tttcactcac cgttaacgca ccggagcggc
    21241 aaacgacgac atccgcccac gcatacgccg ccgccatatc atcaataaat tccgtcactt
    21301 tatgctgcgg ttgccccgct tcggcatacg cctgttcaac ggattgttgc gaacctttgc
    21361 cgctctgatg ccagatagtg actgaatcac ccagtttcgc agcaacctgc ggcattgtct
    21421 gattaagaat gcgtgcgccc tgggaaccac ccactaccaa cacacgaacc ggaccttcac
    21481 gtccagccaa acgttgctgc ggcaacggca gcgccagcac atcggtacgc accgggttac
    21541 ccaccacttc cgcattaggg aaagcacctg gaaacgcctg catcactttg gtagcaatct
    21601 tcgccagcca tttattggtt aagcccgcaa taccgttttg ttcatgaagt acaaccggaa
    21661 tgcctaacga ccacgcggcc agaccacctg gacctgatac atagccgccc ataccgagca
    21721 ccacgtcagg tttgtacgct ttcataatcg cccgcgcctg acgccaggcg ttgaagatac
    21781 gcagcggcgc agctatcagt gcttttatac cttttccacg cagaccagag atacgaatga
    21841 aatcaatttc gatgccatgt tttggcacta agtccgcttc catacggtcg gcagtcccca
    21901 gccagcgaac ttgccaaccc tgagccatta gatggtgcgc aaccgccagc cccgggaata
    21961 catgtccacc ggttccgcct gccatcacca ttaatcgctt cccttgacca ctcatcgtga
    22021 acctcgtaca aacgcctgcg ctttctccag acgcgtttca taatcaatac gcaacagcat
    22081 catgatggct gtcgacataa tcagtaagct cgaaccaccg taactgatca gcggcaatgt
    22141 cagacctttg gtcggtaaca tccccgccgc cgcgcctacg ttaaccagcg cctggaaact
    22201 aaaccagata ccaatagaac aggcgagaaa accggaaaaa cggtggtcaa tttctaatgc
    22261 tttgcggcca atcgacatcg cgcgaaaagc gacgaagaat accattaaaa gtgccagcac
    22321 cacaccgaca taccccagtt cttcgccgat aatggcgaaa ataaagtcag tgtgcgcttc
    22381 cggcagatac tccagttttt gtaccgagtt acctaaacct tgtccccaca gttcaccgcg
    22441 accaaacgcc atcaacgatt gcgttaactg atagccgctg ccaaagggat cttcccacgg
    22501 gttccagaat gcggtaacac ggcggatacg gtacggttcg gcgaggatca gcaacacaac
    22561 cgctgaaatg cccataccga taatggcaat gaactgccac aatttcgctc ccgccaggaa
    22621 caacatcgcc agcgtagtca caaacaacac caccaccgta ccaaggtctg gctgtgccag
    22681 cagtaacact gccaacacca gaattacgcc catcggtttc aggaagccgc gcaggttatt
    22741 acgtacttcg tcgcctttac gcaccagata gttggcgata tagcaaaaca gcgacagttt
    22801 tgtcagctcc gcaggctgaa tacgcagcaa accgagatcg atccaacgcg atgccccttt
    22861 aaccgagcta cccactacca gaacgatcat cagcaggatg atagatccga gcagcatcgt
    22921 ggcactgtag cgttgccaga actccatcgg cagacgcagc gtaatgatcg ccagaatgaa
    22981 tgccaggatc agatagacgc catcacgctt cgcgaagaag aacggatcgt tggttaagcg
    23041 ttgccctatg ggcattgacg ccgaggtcac catgataaag ccaatcgccg cgaggccgaa
    23101 ggtcagccac agtaaggtgc gatcgtacat gatcaggctg tcggtatctt tttcccgcga
    23161 gcccatcacc cagcccttta gcgccgtgga gatccagacc agaatactga atcctggcag
    23221 gcgcggcatt ttcaggcgag ggagagataa acgcatcaac ctaactcctt cgccagacgg
    23281 gcaaactcat tgcctcgttg ttcaaagttc ttgaattgat caaggctggc acaggccggg
    23341 gagagcagaa ccatatcgcc cggctgaaca cgcgtagcaa gcaagcgcat cgcctgttcc
    23401 atagtttcgg tttgttctgc cacttccggg cgtagcgccg ccagctgcgc gccgtcccga
    23461 ccgaaacaat acagacgtac gttatcgcca ttcaggtaac gcgccagtgg gctaaagtcc
    23521 gccgatttac catcgccccc cagcaacaaa tgcagcgtgc cgtctacgtg caggccattc
    23581 agcgccgctt ccgtactgcc gacgttggtc gcttttgaat cgttaaccca gcgcacaccg
    23641 ttatgctcca gcacaacttc aaagcgatgc ggcagaccag tgaatgtggt taacgctttc
    23701 aggctgctgg cacgcggtaa ccctgcggca tctgccagcg ccagcgccgc cagcgcattg
    23761 gtgtagttat gctgcccgga aagtttcatt tctttcacgt tcagcacttt ctcgcctttc
    23821 acccgcagcc aggtttcacc ctgctgatga ttcaggtgat agtcgcccat gttgacgcca
    23881 aagctgacgc agcgttcatc cgcaccgcga atcggcattg ttaaggcatc atcagcatta
    23941 accacgcaaa cttttgcgtt ttcgtaaatg cgcagttttg ctgcacgata ctgttgtaaa
    24001 ccaaacggat agcgatccat atgatcttca gtcacgttca gaatggtcgc tgctaccgcc
    24061 tgtaagctgg aggtggtttc cagctggaag ctcgacagtt ccagcacgta cagttcgcac
    24121 tcatcatcca acagcatcaa cgcaggcagg ccaatattgc cacccacacc aacgttaacc
    24181 cccgccgctt tcgccatttc acccactagc gtggtgaccg tgcttttgcc gttagaaccg
    24241 gtaatcgcca caatcggtgc ttgtgcttca cgacagaaca gctcgatatc gccaacgatt
    24301 tcgattccgg catcagcggc agcgcttaag gatggatgcg ccagtgcaat accgggactg
    24361 gcgacaatca gatctgccgc catcagccat tcatcattca gaccgcccgt gtggcgttct
    24421 acggcttcgg gtaatttatc caggccaggc ggtgtcatac gcgtatccat aacgcgcggc
    24481 gtcacaccgc gagcgaggaa aaagtccacg caagaaagcc cggtgaggcc caggccgata
    24541 atgacgacat ttttaccctg gtaatcagcc atgattaacg taccttcagc gttgccagac
    24601 caatcaggac cagcatcagc gaaataatcc agaaacgcac aatgacgcgc ggttccggcc
    24661 agcctttcag ttcatagtgg tgatgaatcg gtgccatgcg gaaaatacgt tgtccgcgca
    24721 gtttaaagga gccgacctgc aggatgacgg aaagcgtttc taccacgaac acgcccccca
    24781 taatcaccag caggaattcc tgacgcagca gtacggcgat aatacctaac gcaccaccta
    24841 acgccagcga acctacatcg cccataaaga cctgcgccgg ataggtgtta aaccacagga
    24901 agcccagtcc tgccccgact atcgcggtac agacaatgac cagttccccg gcgtgtcgta
    24961 gatacggtat gtgtaaatag ctggcaaagt tcatgttgcc ggtcgcccac gccaccagcg
    25021 caaaaccacc ggcgacaaat acggtcggca taattgccag accgtcgaga ccatcggtca
    25081 ggtttaccgc gttgccagta cccacaatga cgaagtaagc cagcagaatg tagaacaacc
    25141 ccagttgcgg catcacatct ttaaagaatg ggaccaccag ctgcgttgcg ggcgtgtctt
    25201 tgccgacaag gtacagcgcg aaggcgacac ccagcgcaat caccgacatc cagaaatact
    25261 tccaacgggc aatcaggccc ttggtgtctt tacgcaccac tttgcgataa tcatcaacaa
    25321 agccaataac accgtaacct accagcacca ccagtacgca ccagacgtac ggattggagg
    25381 ggtaagccca cagcagtacg gagatcacaa tcgccgtcag gatcattatc ccgcccatgg
    25441 tcggcgtacc gcgcttacta aagtgcgatt ccgggccatc gttacgaacg acctgaccaa
    25501 aggagagttt ttgcaaatga gcaatcatac gcgggcccat ccacaatgag atgaacagcg
    25561 cggtcagcag gctgacgatg gcgcgaaacg tcagatagga aaagacgtta aagccggaat
    25621 aatatttgac caaatgttcg gccagccaaa ctaacatgtc ccattctcct gtaaagcgcg
    25681 tactacctct tccatggcag cactacgtga acccttaact aaaatcgtaa ttacctgttg
    25741 ctcagcaatc agtgatttaa gacgcgcgat aagcgcagtt ttatcggaaa aatgttcgcc
    25801 aacgccgctg gcagtgctga tagcatggct ttgtttaccc acacttaaca cgcagtcaat
    25861 accagcagct tttgccgctt cgcccacctg tacatggcag gcttcgcttt cagcgcccag
    25921 ttccgccata tcgcccacca ccagcacgcg gtagcccggc atttcagcca gaacctgaac
    25981 agcggcggtc attgaaccga cattggcgtt gtaggagtcg tcgagcagca actggttttc
    26041 tgccagtttt atggggaaca gacggcctgg aacagctttc agatttgcca gccccgcttt
    26101 gatagcatca agcgttgcgc ccacggacat ggagagcgca gcggctgcca gcgcattcgc
    26161 aatattgtga cgccccggca acggcagcag aacatcgacg ctacctgtag gggtttgcag
    26221 ggtaaattcc gtaccgtgcg aggttacatg gatattggtg gcggtgaaat cgctgttggc
    26281 gacattaggt gagaaacgcc acactttgcg tgagccaatt acgctctgcc agttcagcca
    26341 gtcgttgttg tcggcgttca taatggcgat accgttttcc ggcaggccgc taaagatttc
    26401 acctttcgct ttcgcgacac ccgcaagcga gccaaaacct tccagatgcg ccgctgccag
    26461 gttgttgacc agcgccgctt ccgggcgagt cagactcaca gtccaggcaa tttccccctg
    26521 atggttcgcg ccaagttcaa taactgcgta atcgtattcc ggcgttaagc gcaacagcgt
    26581 catcggtaca ccgatgtcgt tattgagatt gcctgccgta taaagtgtgt tgccgcactg
    26641 gcttaaaatt gccgccgtca tctctttaac ggaggttttg ccggaggaac ctgtcagagc
    26701 aaccacgcgc gccggaactt gctggcgaac ccatgcagcc agttcaccaa acgccagacg
    26761 cgtatccttg acgattaact gcggcagatc aatatccagc ggacggctaa ccagtagtgc
    26821 gcctgcgccg ccagctttcg cctggtcggc aaaatcatga gcatcgaaac gttcgccttt
    26881 cagggcaaca aacaggcagc ccggcgtcag ttttcgcgtg tcagtggtta cagcatcaag
    26941 ggtaatatct ccaccttgca gttcaccgtt gagaatatcg gtaagttggc taagggttac
    27001 gctaatcatg caatcacccc cagcagacgc gccaccgtga cgcgatcgga gtagtccaga
    27061 cgctgattgc caacaatctg gtaatcctca tggcctttgc ccgcgaccag taccacatca
    27121 ttctctttag cctgcataac ggcgcaagtc accgcttcag cacggccttc catcactttg
    27181 gcatatccgg catctaacat tcccgccaga atatcgttga tgatggcacg aggctcttcg
    27241 gtacgcgggt tatcgtccgt caccaccgcc acgtcagcaa actcttcagc aattgcgccc
    27301 atcagtggac gtttgccttt atcacgatcg ccaccgcagc caaagacgca ccacagcttg
    27361 cctgcacagt gcaggcgcgc cgcctgtaag gctttttcca gtgcatccgg cgtatgcgcg
    27421 taatccacca ccaccgtcgg tttgcctggt gcagtgaaca cttccatacg tccgcaaacc
    27481 ggttgcagac gcgcggcggt tttcagcaaa tcagccagcg gatagccgag tgccagcagt
    27541 gtcgccagcg ccagcagcag gttgctgacg ttgaatgcgc ccatcaggcg actttcaatt
    27601 tccccatcgc cccaactcga gctaaagcga atggtcgcac cgctgtcgtg atagttcacg
    27661 tcggtcgctt tcaaccagcg tccgtgacag ttcggattga tatgatcttc cattgatacc
    27721 gcaaccgcgt ccggcagttt tgccagccag cggcggccca cttcatcgtc ggcgttaata
    27781 atcgcctgac cgcaatgatg tgcagaataa agcagccatt tcgcggcttc gtagtgttcc
    27841 atatcaccat gataatcaag gtgatcgcgg cttaagttgg taaagaccga cgccgcaaat
    27901 ttcaatgccg ccacacggtg ttgtaccagc ccgtgggatg aaacttccat tgcgcaaaaa
    27961 gtcgcgccct gatccaccag ccccgccagc tcatgctgaa catcgactgc cgaaccggtt
    28021 gtattttctg tcgggatcac tttccccagc aggccgttac caacggtgcc cattaccgcg
    28081 ctggtttcgc caagcagttg gctccactgc gccaacagct gggtagtcgt ggttttgccg
    28141 ttggtgcccg ttacgcccac gagacgtaaa ttgtcagagg gttcatggta aaagcggccc
    28201 gccagtgcag ataaacgctc gttgagctgg ctgagataga tgaccggtac gccgtgcatt
    28261 tcacggattt caccgtcggt cgcctcatct ttcgcctctg caataatggc agccacacct
    28321 tgcgctatcg cctgcgggat atatcgacgc ccgtccgcct gatgacctac tacagctaca
    28381 aagagatcgc ccgccgcagc cacacggctg tcgagtgtca tctctcgcag tgctcgcgaa
    28441 ggtgcgtctg gcacccacgg agcaagaagg tcgcgcaaat tacgatctgc cacctgtacc
    28501 ctcgccttga ttaatcacaa attcattttt atcgcccgtt gtcagcgcat ccggctcgat
    28561 gttcatggta cgcaatacgc cgcccatgat ggcaccaaag accggcgcgg aaacggcgcc
    28621 gccgtagtat ttacccgcct gcggatcgtt gataacaaca accagcgcga agcgcggctg
    28681 actcgcaggc gcaacgcctg cggtataagc aatatattta ttgatgtagc gaccgtccgg
    28741 cccgaccttt ttcgcggtac cggttttaat ggcgatacga tagcctttaa tcgccgcctt
    28801 cacgccgccg ccgcccggta gcgccacgct ttccatcata tgcaccacgg tgcgaacaat
    28861 ggattccggg aagacacgtt caccgggaac cggggggtca actttggtaa tcgacagtgg
    28921 gcgataaatg ccgtagctgc cgatagttgc gtagactcgc gctaactgta acggtgttac
    28981 cattagcccg tagccgaaag agaaggtggc cctctctatg tcagaccacc gttgtttttg
    29041 aggatataag ccactgcgtt ctccgaccaa ccccaaattg gtcgcttttc ccagcccaaa
    29101 acgtgagtaa gtatctacta acgctgagga cggcatcgct aacgccagtt tggaaacacc
    29161 gacgttactc gacttctgta ataccccggt cagggttaat tcgctgtagc gtgccacgtc
    29221 tttgatttcg tggccgttaa ttcgataagg aacggtattg agtaccgagt tttcccgcac
    29281 cacgccacgt tgcaacgcgg tcatcaccac catcggttta accgttgagc ccggttcaaa
    29341 cacgtcggtg atggtacggt tacgcatcgc ttctttcggc gtgccgctca gattatttgg
    29401 gttgtatgac gggctgttag ccatcgccag cacttcaccg gtgttgacat ccaccagcac
    29461 ggcgctacca gattcagcct tgttaaaggc caccgcgttg ttcagttcgc gataaaccag
    29521 cgcctgcagg cgttcatcaa tactcagcgt caggttgtgc gctgcctggc tgtcagtaga
    29581 agaaatatct tcaattacgc gaccatagcg gtctttacgc acaatgcgct cacccggctg
    29641 cccggtaagc catttatcga aactcttctc gacgccctca atcccttggc tatcgacgtt
    29701 agtaaagccg atgaggtgag cagtcacttc gccggatgga taataacggc gagattcttc
    29761 acgcagatga atccccggca gtttcagttt tttgatgtag tccgccatgt cagggtttac
    29821 ctgacgcgcc agataaataa agcgcccttt cgggttggcg ttaatgcggg ctgagagctg
    29881 atccagcgga atattgagcg cgttagccag cgccttccag cggtcaccga cgctgatacc
    29941 gccagcgtca tgcacttctt tcgggtcagc ccaaatcgct tttactggca cactcaccgc
    30001 taacgggcga ccagaacggt cagtaatcat gccgcgggag gtggaaactt gctgaacgcg
    30061 aagagaacgc atgtcgccct ctttcaccag catatccggg gagataactt gtaaccacgc
    30121 tacgcgtccg agcagaaaag ccagcgccag gagaatacag ccgcataaca acgcaaaacg
    30181 ccaactgata aagttggcat gttcttcctg acgttttggt ttctgcgttt tcgccgctgc
    30241 tttcatgcgt cgcgtttatc cttatttttg cactacgata ttttcttgtg acggatcaac
    30301 atgctgcatt tgcagctttt ccgtggcgat cctttccacc cggctatggt cgccgagcgc
    30361 attctcttca aggatcaggt tgcgccattc aatgtctaaa gcatctcgct ccagcaccag
    30421 ttgttcgcgc tgagcggtca gtaaacgggt atggtgcgcc gtggttacca cggtcaccgc
    30481 cgtcaaaata atgcaaatga acaggcagag tggcagcttc ccaaatcgca aaagatcgtc
    30541 accgataaca ccaggcaatg catggcgctc gtggcttccc atcgatcctt taactttgct
    30601 tagagcttct gtcactctgc tgatcatgcg ttcgtcctct ctgcaatacg cagaactgaa
    30661 ctacgggcac gagggttctc agccacctct tcttcgcccg gcattaactt gcctagtgct
    30721 cgcagctgac ggccacccag ttttttgagc tgctcttcag tcatcggtaa ccctgccgga
    30781 acttgcggac cgcggctgtt ttcacgcata aaacgtttca caatacggtc ttccagcgag
    30841 tggaagctga tgatcgaaag ccgccccccc ggggccagca cgttgagcga gctttttagc
    30901 gcctgctcta tctcctccag ttcactgttt acccaaatgc gcaccgcctg gaaggtacgg
    30961 gtcgcgggat gtttaaactt atctttcacc ggcgttgcag cagccacgac ttccgccagt
    31021 tctttggtgc gggtcatcgg ctgttcgcgg ttacgctcga caatggcgcg ggcaatgcgt
    31081 ttggcaaaac gctcttcacc ataggttttc aatacccagg cgatatcggc ttcttctgcg
    31141 gtttgtagcc attcagcggc tgactgccca cgggttgggt ccatacgcat gtccagcgga
    31201 ccatcgcgca taaaggaaaa gccgcgctca gcatcatcaa gttgcggtga agagacgcca
    31261 agatcgagga gaatgccgtc gatcttgccg ataagatcgc gctcggcaac atattcgccc
    31321 agcgcggaga aaggtccgtg gatgatggag aagcgcggat catcaatagt cttcgcaacg
    31381 gcgatagcct gcgggtcgcg atcgatcgcc agcaaacgcc cctcttcgcc aagctgcgag
    31441 aggatcagac gtgagtgacc accgcgacca aaagtcccat caatgtagat gccatcagga
    31501 cggatattga ggccattaac ggcttcatcc agcagcaccg tagtatgttt atagttttcc
    31561 atcattttat agagacaagt cctgcagtcg ctccgataag tctccggtag ccaactgctc
    31621 tgcgtcgata tcttccttga cctgttgatg ccaggttgtt tcatcccaca gctcaaactt
    31681 gttgaactgt ccaaccagca tcacttcttt tgtcagcccg gcatgttgcc gcagtactgg
    31741 cgcgattaac aatcgacctg cgccatccat ctgacattcg ctggcatgac ctaacagtag
    31801 gcgctgcaca cggcgctcaa ccgggttcat gctcgacaga cgcgataatt tttgctcgat
    31861 aatttcccat tcaggcaggg ggtaaagcag caggcacggg tgatgaatgt caatggtgca
    31921 aaccatttga ccggcagcgt tctcaagcag cggttcccga taacgggtag gcactgataa
    31981 gcgccctttg ctgtcgagat tgactaacgt tgctccccgg aacatgccag cctcacccct
    32041 tattcccact ttgccccaca atttcccact gaaaggagtt tacggagcgg aggaaaagct
    32101 tgtcaagcca gtcacaaggc ataccgaggt aaaaaacccg atgtttacgg ctaatatcag
    32161 cgttgtttat tttacgtcca acgtgcgaag caaattaacg ttatgaatag ctgtaagaaa
    32221 aaatccctca tcgtcatcac cgttttaaaa tgactcacta ttttccacag caaatataaa
    32281 gtgtcagttt gcgacgcgag cggcatttta ggacatatct tctctcgtta acagtccctg
    32341 ttgcgtctgt gtggcgcttg atgccagcgg gtttgcgcaa aagcaaagaa aagtgtttgt
    32401 taaatcggca attcatctct gcatgtaaca aaataatgca aaaacgcgct atcgatatca
    32461 cttaattatt gtgaatttca gctggtgagg taattaacgg gcaggtttaa aagcatttat
    32521 caataatagc caggaatagt cttatttact ttaaggatat tgatgtccat tcccgtactc
    32581 aacgcgccag agagaaattc acctggcgcg tatttttgtt cgcggcttag ctacggctga
    32641 gcacgccgcg gcgatagaga ttacgtttaa tgcgcgttaa accaggtttt ggcttacgcg
    32701 gttcgtccag gcttgccagg acaatctcca gcacacgctc tgcgacatcg cggtgacgtt
    32761 gagccactgc cagcaccggg cactgtaaga agtcgagcag ttcgttatcg ccaaaggtgg
    32821 caattgccag gtcagaaggc agtttgccgt cacgacgtag cgtgacatcc atcacccctt
    32881 gcaacaacgc aaacgacgtt gtgaacagtg cctgcggcat cggatgcgtt tccagccatt
    32941 tttcgaataa ctgggcagcc gcctcccgct catagctgtt ggcataaagg aaatgcactt
    33001 cgcgcggatc atctttccag gcagtacgga aaccttgttc acgcaggaag ctgacagaaa
    33061 gctccggcag cgcgccaaga taaagtaccg tctcggcggg aaacttacgt aactcttccg
    33121 ccagcatttc ggcatcatcc tgatcggcac caaccacgct ggtgaaatgt tcacgatcga
    33181 gggcgcggtc cagcgcgaca atcgggaacg ggtcgttagc ccagcgttga taaaaaggat
    33241 gctcaggtgg caacgacgtc gaaacaataa tggcatcaac ctgacgctgt aaaagatgct
    33301 cgatacaacg catttcgttg tcgggctgat cttccgagca ggcaatcagc agttgataac
    33361 ccctttgccg cgcctggcgt tcaagatagt tagcgatgcg ggtatagctg gtgttctcca
    33421 gatcggggat cacaagacca atagaacgtg tgcgtccagc acgaagccca gctgccacgg
    33481 cgttcgggtg ataattgtgc tcacgcacta ctgccatgac tttttcaacg gttttgtcgc
    33541 tcacacggta ttgcttcgct ttgccgttaa taacatagct tgcagtggtc cgcgacactc
    33601 ccgccagccg agcgatttca tccagtttca caattgcccc ttgcgtaaaa tgtaaaaacc
    33661 atagccattg tactggtatg ggttaaattc attaacatct aagcgcagaa aattaactgc
    33721 ggcaactgct tttattccag gttcccactg atttcgcaaa aaaagcccaa cgtgacatgt
    33781 tgggctgcaa attgcgcatt gagatcattc tcaacgcatt attttatcgc cgcgtgaaag
    33841 tccgaccaca ccagagcgag caacttccac aattttcgcc acatcgcgaa tcgatgctaa
    33901 aaatgcatca agcttatcgc tggtgcctgc taattgaacg gtataaagcg agggtgtgac
    33961 atcgataatt tgcccacgga atatttccgt attacgtttc acttcgtcac gcccgtaacc
    34021 gctggcctga attttcacca gcatgatttc ccgctcaacg tgcgcgccct gccccaactc
    34081 gctcacgcgc aagacatcga ccagcttgtg taattgcttt tcgatttgct caagcacttt
    34141 ttcatcgccc acggtctgaa tggtcatacg cgataatgtc ggatcgtcgg ttggcgcaac
    34201 ggtcaggctt tcaatgttgt agccacgctg ggaaaaaagg ccaatcacgc gggataacgc
    34261 gcctgattca ttttcgagta agactgataa tatccggcgc ataatcaggt tctctccgtt
    34321 ttgcttaacc acatttcatc cattccgccc ccgcgaatct gcatcgggta gacgtgctca
    34381 ctgccatcga cggtgacatc aacaaacacc aggcgattat tgcgcacctg ttccagcgcc
    34441 tcgctaagtt tgctttccag ttcttgcgga tgagagatct ggatcccgac gtgaccatag
    34501 gcttccgcca gacgaacgaa atcgggtagt gactgcatat aagattgtga atgacggccg
    34561 gaatagatca tgtcctgcca ctgttttacc atccccagat agcggttatt gagattcacc
    34621 accagtacgg gcaactcgta ttgcaacgcg gtagacagtt cctgaatgtt catctgaata
    34681 ctgccgtcgc cagtgacgca aaccacggtt tcttctggca acgccatttt gacgcccagt
    34741 gccgcaggta aaccaaaacc catcgtgccg aggccgccag agttgatcca gcgacgcggt
    34801 ttgtcgaatg gataataaag cgcagcaaac atctggtgct gcccgacatc ggacgtcacg
    34861 taagcgtctc ccttcgtcaa ccgccaaaga gtctcgatca ccgcctgcgg tttaatcttt
    34921 tcactgtgag tgtcatattt caggcactga cgagcgcgcc actgttcaat ctgctgccac
    34981 cagtcgcgga tctcatccag cggttgatgg gcggattctt gcgacaggag ttcaagcatt
    35041 tgttcgagga cctggcgagc atcccccaca atcgggatat cagcagtcac ggttttagaa
    35101 attgaggttg gatcaatatc gatatgcaga acagtggcat ttgggcagta ctttgccaga
    35161 ttgttcgtcg ttcggtcatc aaaacgtacc ccgacggcga aaatcacatc cgcgttatgc
    35221 atcgtcatat tggcttcgta ggtaccgtgc attcccaaca tgcccagtgc ctgacgatgc
    35281 gttgccggaa acgcccccag ccccatcaaa gaggaaacaa cgggcagatt caacgcctcc
    35341 accgcttctt tcaactgcag atggcagccc gccgtgattg ccccaccgcc tacgtagaca
    35401 accggttttt ttgccgccac cagcgtttgc agagcgcgct taatttgccc tttatgcccg
    35461 gaggttgtgg gattgtaaga acgcatactg accgactccg gccagacata gggtaatttg
    35521 ttcgccggat ttagaatatc tttcggtaaa tcaacgacca cgggtccagg acgaccactt
    35581 gccgctagcc agaaagcctt tttcagcacc tgtggaatgt cttccgtttg cttaaccaga
    35641 aaactgtatt taaccaccgg gcgagaaatc cccaccatgt cgcactcctg aaaggcatca
    35701 taacctatca acgaagtcgc tacctgcccg gaaaggacaa ctaatggaat ggaatccata
    35761 taagcggtgg caatgccagt aatcgcattg gtcgcccctg gacccgacgt taccagcacg
    35821 acgccgactt ccccggtcgc gcgcgccaga ccatcggcca tatgcaccgc cgcctgctca
    35881 tggcgaacta aaacatgatc gataccaccc acggtatgca atgcatcgta aatatcaagg
    35941 accgcgcctc caggataacc gaatacttgt ttaacgccct gatcgataag cgatcggacg
    36001 accatctcgg ctccagacaa catctccatg gcctgcctca ctgtttgacg gaaaaaatgt
    36061 gtaaaacaca ttaaccgtcc gatcttgctt aagcaattgg ccgacagaga cataaacacg
    36121 gggtaataag aataaacagg aggaaaggtg aaaagaaacg ataaaatcct ccattacaga
    36181 ggatgaaatt gataataaat cggctgaatc ctacaactta cagcaatttc tctatttttt
    36241 taattaatcc taaaatagag agcacaaaca ttcagaataa aattctacat tcaataaaaa
    36301 atgcagaata aaccagacat tgatgtccga cctattctgc attcagttag cgtttgcaaa
    36361 ttgagactaa ttgctcttcc atccactgat ggcctttatc gcgcccggca gcttcatgcc
    36421 aggagagata acaggttctg ctgttttgtt ttaacggcag cggtaatacc tgtaactcta
    36481 aggattcagc gaactcttca gccaaccaac gcggcgcaat agcgaccaga tgcgtttgcg
    36541 acaccacgct aagtacgctc atcattgcca tgccctgata cgcgatactg gcttgcttat
    36601 ctaccgtgtc ataccaggtt tgactaaatg atgcgaaacg atcgagcgaa accgcagcat
    36661 gttgttcgtt ataaacatca tgtttcagta acgggccttt aattgttgga tgatttttgc
    36721 tggctaccaa caccatttca tctttaaata atggcacact ggtaaattca gggcgatgga
    36781 agtcttcata actaatcaca aactccgttt cctgataacg cagctgatgt tcagtgttct
    36841 gatttaatga agacttgaac ataacatgta tatttggcgc aatctgctca atgtgattat
    36901 aaatctgcga ggtcagaatg ctgtctaacg ggctgcaaac acaaagatga aatacacgtt
    36961 cactgctcgc gggttcaaaa cctgaaccag gcaattcatt ttgtactagt tgcaatgcct
    37021 gacgaactga gccaaaaagt tgaaatgcgc gagcagtcgg ttgaatacca cggccataac
    37081 gaacaaaaag ctcgtcatta aacatcacct tcaggcgtgc aacagcgtta ctgaccgcag
    37141 gttgcgacat tcccagagca tgagcggcac gggtgatgtt ttgctcctgc atcacggcat
    37201 cgaaaacggt taataagttg agatcgacca tgcgtagctg tggcttgctt aactccgccg
    37261 tctctggatg atctgtttgt acctctggca tacttaactc cactgtcaca cttaactccc
    37321 tttcccttat tggaatgcag agaaataatc ctcgagaata tgatttatca tgcatataaa
    37381 ataagaaaaa gcaaaatgag taaaattagg aaaacataaa gatacaggtt ctgataaaaa
    37441 cagaatccac tgccgagcaa agagtcattt actacagcat aataatccat aatgtttttg
    37501 ttgcgaaaac aatctaatca taactaccac gaatactcaa tcatccacaa aatggattaa
    37561 atgtgatttt taataattaa tttatatgtt taatgcatta a
//
LOCUS       DAZKHK010000035        37346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKHK010000035 DAZKHK010000000
VERSION     DAZKHK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.035"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            144..1073
                     /gene="pfkB"
                     /locus_tag="ACHOS5_001803"
     CDS             144..1073
                     /gene="pfkB"
                     /locus_tag="ACHOS5_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP9776624.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1174..1464
                     /gene="ghoS"
                     /locus_tag="ACHOS5_001804"
     CDS             1174..1464
                     /gene="ghoS"
                     /locus_tag="ACHOS5_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9776625.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1570..2430
                     /gene="yniA"
                     /locus_tag="ACHOS5_001805"
     CDS             1570..2430
                     /gene="yniA"
                     /locus_tag="ACHOS5_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9776626.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEIHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLSMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2471..3007)
                     /gene="yniB"
                     /locus_tag="ACHOS5_001806"
     CDS             complement(2471..3007)
                     /gene="yniB"
                     /locus_tag="ACHOS5_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP9776627.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNVFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PLICIAAGYVFFSLLGFI"
     gene            3154..3822
                     /gene="hxpB"
                     /locus_tag="ACHOS5_001807"
     CDS             3154..3822
                     /gene="hxpB"
                     /locus_tag="ACHOS5_001807"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP9776628.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3986..4576
                     /gene="ydjM"
                     /locus_tag="ACHOS5_001808"
     CDS             3986..4576
                     /gene="ydjM"
                     /locus_tag="ACHOS5_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9776629.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4709..6100
                     /gene="tcyP"
                     /locus_tag="ACHOS5_001809"
     CDS             4709..6100
                     /gene="tcyP"
                     /locus_tag="ACHOS5_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP9776630.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6147..6410)
                     /gene="cedA"
                     /locus_tag="ACHOS5_001810"
     CDS             complement(6147..6410)
                     /gene="cedA"
                     /locus_tag="ACHOS5_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP9776631.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            6593..8854
                     /gene="katE"
                     /locus_tag="ACHOS5_001811"
     CDS             6593..8854
                     /gene="katE"
                     /locus_tag="ACHOS5_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP9776632.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADSADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            complement(8901..9659)
                     /gene="chbG"
                     /locus_tag="ACHOS5_001812"
     CDS             complement(8901..9659)
                     /gene="chbG"
                     /locus_tag="ACHOS5_001812"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP9776633.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFVAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELHVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(9672..11024)
                     /gene="celF"
                     /locus_tag="ACHOS5_001813"
                     /gene_synonym="chbF"
     CDS             complement(9672..11024)
                     /gene="celF"
                     /locus_tag="ACHOS5_001813"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9776634.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDCDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(11127..11969)
                     /gene="chbR"
                     /locus_tag="ACHOS5_001814"
     CDS             complement(11127..11969)
                     /gene="chbR"
                     /locus_tag="ACHOS5_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP9776635.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVVSQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(11977..12327)
                     /gene="chbA"
                     /locus_tag="ACHOS5_001815"
     CDS             complement(11977..12327)
                     /gene="chbA"
                     /locus_tag="ACHOS5_001815"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP9776636.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(12378..13736)
                     /gene="chbC"
                     /locus_tag="ACHOS5_001816"
     CDS             complement(12378..13736)
                     /gene="chbC"
                     /locus_tag="ACHOS5_001816"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP9776637.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(13821..14141)
                     /gene="chbB"
                     /locus_tag="ACHOS5_001817"
     CDS             complement(13821..14141)
                     /gene="chbB"
                     /locus_tag="ACHOS5_001817"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP9776638.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(14440..14778)
                     /gene="osmE"
                     /locus_tag="ACHOS5_001818"
     CDS             complement(14440..14778)
                     /gene="osmE"
                     /locus_tag="ACHOS5_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP9776639.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            14980..15807
                     /gene="nadE"
                     /locus_tag="ACHOS5_001819"
     CDS             14980..15807
                     /gene="nadE"
                     /locus_tag="ACHOS5_001819"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9776640.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            16037..16924
                     /gene="cho"
                     /locus_tag="ACHOS5_001820"
     CDS             16037..16924
                     /gene="cho"
                     /locus_tag="ACHOS5_001820"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP9776641.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(16884..17459)
                     /gene="ves"
                     /locus_tag="ACHOS5_001821"
     CDS             complement(16884..17459)
                     /gene="ves"
                     /locus_tag="ACHOS5_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP9776642.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLGGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(17662..18147)
                     /gene="spy"
                     /locus_tag="ACHOS5_001822"
     CDS             complement(17662..18147)
                     /gene="spy"
                     /locus_tag="ACHOS5_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP9776643.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(18477..19445)
                     /gene="astE"
                     /locus_tag="ACHOS5_001823"
     CDS             complement(18477..19445)
                     /gene="astE"
                     /locus_tag="ACHOS5_001823"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP9776644.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(19438..20781)
                     /gene="astB"
                     /locus_tag="ACHOS5_001824"
     CDS             complement(19438..20781)
                     /gene="astB"
                     /locus_tag="ACHOS5_001824"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP9776645.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKKLAGAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMVLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(20778..22256)
                     /gene="astD"
                     /locus_tag="ACHOS5_001825"
     CDS             complement(20778..22256)
                     /gene="astD"
                     /locus_tag="ACHOS5_001825"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP9776646.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLFLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGSPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(22253..23287)
                     /gene="astA"
                     /locus_tag="ACHOS5_001826"
     CDS             complement(22253..23287)
                     /gene="astA"
                     /locus_tag="ACHOS5_001826"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP9776647.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDGETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(23284..24504)
                     /gene="astC"
                     /locus_tag="ACHOS5_001827"
     CDS             complement(23284..24504)
                     /gene="astC"
                     /locus_tag="ACHOS5_001827"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP9776648.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATKECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQLSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            24950..25756
                     /gene="xthA"
                     /locus_tag="ACHOS5_001828"
     CDS             24950..25756
                     /gene="xthA"
                     /locus_tag="ACHOS5_001828"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9776649.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            25923..26633
                     /gene="ydjX"
                     /locus_tag="ACHOS5_001829"
     CDS             25923..26633
                     /gene="ydjX"
                     /locus_tag="ACHOS5_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9776650.1"
                     /translation="MNAERKFLFACLIFALLIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFKAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            26638..27315
                     /gene="ydjY"
                     /locus_tag="ACHOS5_001830"
     CDS             26638..27315
                     /gene="ydjY"
                     /locus_tag="ACHOS5_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP9776651.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            27329..28036
                     /gene="ydjZ"
                     /locus_tag="ACHOS5_001831"
     CDS             27329..28036
                     /gene="ydjZ"
                     /locus_tag="ACHOS5_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9776652.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            28036..28584
                     /gene="ynjA"
                     /locus_tag="ACHOS5_001832"
     CDS             28036..28584
                     /gene="ynjA"
                     /locus_tag="ACHOS5_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9776653.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            28594..29760
                     /gene="ynjB"
                     /locus_tag="ACHOS5_001833"
     CDS             28594..29760
                     /gene="ynjB"
                     /locus_tag="ACHOS5_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9776654.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQTLLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            29733..31268
                     /gene="ynjC"
                     /locus_tag="ACHOS5_001834"
     CDS             29733..31268
                     /gene="ynjC"
                     /locus_tag="ACHOS5_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9776655.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLILLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWQEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            31268..31921
                     /gene="ynjD"
                     /locus_tag="ACHOS5_001835"
     CDS             31268..31921
                     /gene="ynjD"
                     /locus_tag="ACHOS5_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9776656.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFSVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            31988..33295
                     /gene="ynjE"
                     /locus_tag="ACHOS5_001836"
     CDS             31988..33295
                     /gene="ynjE"
                     /locus_tag="ACHOS5_001836"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP9776657.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(33304..33924)
                     /gene="ynjF"
                     /locus_tag="ACHOS5_001837"
     CDS             complement(33304..33924)
                     /gene="ynjF"
                     /locus_tag="ACHOS5_001837"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9776658.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALVGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            34011..34418
                     /gene="nudG"
                     /locus_tag="ACHOS5_001838"
     CDS             34011..34418
                     /gene="nudG"
                     /locus_tag="ACHOS5_001838"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP9776659.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            complement(34384..34656)
                     /gene="ynjH"
                     /locus_tag="ACHOS5_001839"
     CDS             complement(34384..34656)
                     /gene="ynjH"
                     /locus_tag="ACHOS5_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP9776660.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            34892..36235
                     /gene="gdhA"
                     /locus_tag="ACHOS5_001840"
     CDS             34892..36235
                     /gene="gdhA"
                     /locus_tag="ACHOS5_001840"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9776661.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPT
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            complement(36291..>37346)
                     /locus_tag="ACHOS5_001841"
     CDS             complement(36291..>37346)
                     /locus_tag="ACHOS5_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9776662.1"
                     /translation="NAIKLKGLFYYPSSVYQSCLHAAEQGYSLAWYSLGKMNYEGDEV
                     SQDLKLAFNWFTRAAQHNVIDALYALGIMYSDGRGTDKNICEARKWFLLAAQNGNTSA
                     QYELARISRFAVEPLRNYEEALQWYLSAATQGHEVAQYELGQMYIQGIGVERDEVQAH
                     RWFLQSAEQGYLHAQYHTARLYSGSESIPQDQEKALYWFTKAAKNGADGAGDAMYELG
                     KYYLTNNDDPENNAEATQWLTGAAQRGRIEAIFLLAEMYLYGTKYIAKDENHALHWYE
                     KAARLGATEAQHQTAAMYAQGTGTKIDNKQAWMWLTIAGNNNPWIEKDALRCLMSEQD
                     IQDAEQTAANCKRLLRI"
BASE COUNT         9211 a         9755 c         9178 g         9202 t
ORIGIN      
        1 aataagcatg tggcgaaaac agatttttat ttatatatat ttatctgcaa aattttaagt
       61 aaagctccaa taaatcatat tgttaatttc ttcactttcc gctgattcgg tgccagactg
      121 aaatcagcct ataggaggaa atgatggtac gtatctatac gttgacactt gcgccctcac
      181 tcgatagcgc aacaattacc ccgcaaattt atcccgaagg aaaactgcgc tgtaccgcac
      241 cggtattcga acccggaggc ggcggcatca acgtcgcccg cgccattgcc catcttggag
      301 gcagtgccac agcgatcttc ccggcgggtg gtgcgaccgg cgaacatctg gtttcactgt
      361 tggcagatga aaatgtcccc gtcgctactg tagaagccaa agactggacc cggcagaatt
      421 tacacgtaca tgtggaagca agcggtgagc agtatcgttt tgttatgcca ggcgcggcat
      481 taaatgaaga tgagtttcgc cagcttgaag agcaagttct ggaaattgaa tccggggcca
      541 ttctggtcat aagcggaagc ctgccgccag gtgtgaagct ggaaaaatta acccaactga
      601 tttccgctgc gcaaaaacaa gggatccgct gcatcatcga cagttctggc gaagcgttaa
      661 gtgcagcact ggcgattggt aacatcgagt tggttaagcc taaccaaaaa gaactcagtg
      721 cgctggtgaa tcgcgaactc acccagccag acgatgtccg caaagccgcg caggaaattg
      781 ttaatagcgg caaggccaaa cgggttgtcg tttccctggg tccacaaggg gcgttgggcg
      841 ttgatagtga aaactgcatt caggtggtgc caccaccggt aaaaagccag agtaccgttg
      901 gcgctggtga cagcatggtc ggcgcgatga cactgaaact ggcagaaaat gcctctcttg
      961 aagagatggt ccgttttggc gtagctgcgg ggagtgcagc cacactcaat cagggaacac
     1021 gcctgtgctc ccatgacgat acgcaaaaaa tttacgctta cctttcccgc taacaaaaac
     1081 attccccctg cattggggga atcaccacca acctgtcggc aacgcgtttc tccgactatg
     1141 ctcaaaagtc atgtgataac aaaggggtga actatggcca gtggcgatct tgtccgttat
     1201 gtcataaccg tcatgttgca cgaggataca ttgactgaaa ttaacgagtt gaataattac
     1261 ctgactcgcg acggtttttt gctcaccatg acggatgatg atggcaatat ccatgagctg
     1321 gggactaaca cttttggact tatcagtacc caaagtgaag aagaaattag agaactggtt
     1381 tcggggctta cccaaagtgc aaccggtaaa gatcctgaaa tcaccatcac gacctgggag
     1441 gaatggaata gcaacagaaa ataaatggtt tttgggcaat aatcagtctg tggtgtgcgt
     1501 tagctcgtgt ttttacaccg cattcttgcg ctaaccttat gatctggcag acaacatggg
     1561 agagacatca tgtggcaggc aatcagtcgt cttttgagcg agcagttagg tgaaggcgaa
     1621 atcgaactgc gtaatgaact gcctggcgga gaaatccatg ccgcatggca tttgcgctat
     1681 gcaggacgtg actttttcgt caaatgtgat gaaagggaac tgcttcccgg ttttaccgcc
     1741 gaagccgacc aactggagtt actgtcgcgt agtaaaaccg tcaccgtgcc taaggtttgg
     1801 gcagttggcg ctgaccgtga ctacagtttt ctggtgatgg actatctccc acctcgcccg
     1861 ctggatgcgc atagcgcatt tattcttggt cagcaaattg cgcgtttaca tcaatggagc
     1921 gaccaaccgc aatttggcct cgatttcgat aacgcgctct ccacaactcc ccagcccaac
     1981 acctggcaac gtcgctggtc aacttttttt gctgaacaac ggattggctg gcagctggaa
     2041 ctggcggcag aaaaagggat cgctttcggc aatatcgatg ccatcgtcga gcatattcag
     2101 cagcgtctgg cctcgcatca accgcagcct tctctgttgc acggcgattt atggtccggc
     2161 aactgtgcgc tgggtccgga tggcccgtac attttcgacc cggcctgcta ctggggtgac
     2221 cgagagtgcg acctgtcgat gttaccgctg catacagaac agccgccaca aatctatgac
     2281 ggctatcagt cagtatcccc gctacctgcc gatttccttg aacgtcaacc ggtttaccaa
     2341 ctctacacgc tattaaatcg tgcaaggtta tttggcggtc agcatttggt tattgctcag
     2401 cagtcactgg atagattatt agcagcatga tatgggttga ggataatggc cgctccgtgc
     2461 ggccttttga ttaaataaac ccaagcagag aaaagaacac ataacctgca gcaatacaaa
     2521 taagcggcag gatatacagc gagaaaaact gcaggaaaat agtatggtgc ggaacaacaa
     2581 tacgtgactc aatttgttca cgcgttagcc cctcttcccc tttggctttt tccagaatga
     2641 gttgatcttc aacaccttcc cgcaagaagc gcgcctggcg actcatccgg gctccggaat
     2701 cctgcaacgc cagtccgaca aaaatcagga tgaaaatcac ccagaacata acgttcacgc
     2761 caccattaaa attgggcgtc ggagagttgt accagaacac attcaaaaaa ggcgtattcg
     2821 cctgcatcat atcgatcatg acatgagtaa agtcgagcat gaccgcatta atgccttcct
     2881 ggttttcctg ccgggtattc ataaatttca gcaaagaaat caatgtagag atcaatgcag
     2941 gtataaaaat cacccaaccc aaaatccttt tcaaaacagc aatgcgtcca gcttgttgat
     3001 acgtcatgag ttctccttga ttaagacgcg tcgtttcact tagtttacct gtagatatct
     3061 gttttcgccc attctttaaa ggcgatatga taggcgctta atcataagca cggcttaata
     3121 ccttacatat aatgctctaa aggagaggtt gtaatgtcaa ccccgcgtca gattcttgct
     3181 gcaatttttg atatggatgg attacttatc gactcagaac ctttatggga tcgagccgaa
     3241 ctggatgtga tggcaagcct gggggtggat atctcccgtc gtaacgagct gccggacacc
     3301 ttaggtttac gcatcgatat ggtggtcgat ctttggtacg cccggcaacc atggaatggg
     3361 ccaagccgtc aggaagtagt agaacaagtt attgcccgtg ccatttcact ggttgaagag
     3421 acacgtccat tattgccagg tgtgcgtgaa gccgttgcgt tatgcaaaga acaaggttta
     3481 ttggtggggc tggcctccgc gtcaccgctg catatgctgg aaaaagtatt gaccatgttt
     3541 gatttacgcg acagtttcga tgccctcgcc tcggctgaaa aactgcctta cagcaagccg
     3601 catccgcaag tatatctcga ctgcgcagca aaactggggg ttgaccctct gacctgcgtg
     3661 gcactggaag attcggtaaa tggcatgatc gcctctaaag cagcccgcat gcgttccatc
     3721 gtcgttcctg cgccagaagc gcaaaatgat ccacgttttg tattagcaaa cgtcaaactc
     3781 tcatcgctga cagaactcac cgcaaaagac cttctcggtt aatgaccagg gggcagtgat
     3841 cacactgccc ctggttcttt atctgaattt gcattcaact gacggattaa tcgccaattt
     3901 aagagaaaga gttacaccgt caccacttcc gtgcactgta taaaaaccct atactgtacg
     3961 tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt caccttctct tttctattgc
     4021 ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg ctggcacagg gtgactggtg
     4081 gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg gacatcgatc acccaaagtc
     4141 gtttcttggg cagcgattaa agtggatatc aaaaccgatc gcccgcgctt ttgggcatcg
     4201 tggttttacc cacagtctgc tggcggtatt tgcgctgctg gcaacctttt accttaaagt
     4261 tcctgaaagc tggttcattc cggctgatgc gttgcagggt atggtgctgg gttatttgag
     4321 ccacatactt gccgatatgc tgacacccgc cggtgttccc ctgctctggc catgccgctg
     4381 gcgtttccgc ttgcctatcc tggttcccca aaagggcaac caactggaac gttttatctg
     4441 catggcatta tttgtctggt cggtatggat gccccattca ttacccgaaa acagcgctgt
     4501 tcgttggtca tcgcaaatga tcaatacctt acagatccag tttcatcggc ttattaagca
     4561 tcaggttgaa tactaaaaag gcaaaaatcg cctttctgga ataagcaatt ccatttgaat
     4621 ataagagcca gctcacagtt ctgttaatct tgcgccaaca ctatgactgt tacgcagtga
     4681 tagaaataat aagatcagga gaacggggat gaactttcca ttaattgcga acatcgtggt
     4741 gttcgttgta ctgctgtttg cgctggctca aacccgccac aaacagtgga gtctggcgaa
     4801 aaaagtgctg gtgggtcttg tgatgggtgt ggtttttggc cttgccctgc acaccattta
     4861 tggttctgac agccaggtac ttaaagattc tgtacagtgg tttaacattg ttggtaacgg
     4921 ctatgttcaa ctgctgcaaa tgatcgttat gccgctagtc ttcgcctcta ttctgagcgc
     4981 ggttgcccgt ctgcataacg catctcagtt aggcaaaatc agttttctga ccatcggtac
     5041 gcttttgttt accacgctga ttgcagcgct ggtcggtgtg ctggtcacca acctgtttgg
     5101 tttgacggct gaaggtctgg ttcagggtgg tgcagaaact gcacgtctga acgccatcga
     5161 aagtaactat gttggtaaag tctctgacct gagcgttccg cagttggtct tgtcctttat
     5221 cccgaaaaac ccgtttgccg atctgaccgg agccaatccg acgtcaatta tcagcgtggt
     5281 aatttttgcc gcattcctcg gcgtagctgc gctgaaactg ctgaaggatg atgcgccgaa
     5341 aggtgaacgc gtcttaaccg ctatcgatac cctgcaaagc tgggtgatga aactggttcg
     5401 cctggtcatg cagttgaccc cttacggtgt tctggcgctg atgaccaaag tggttgcagg
     5461 ttctaacctg caagacatca tcaaactggg aagtttcgtt gttgcgtcct acctcggtct
     5521 gctgattatg tttgcagtgc atggccttct gctgggcatt aatggcgtga gcccgctgaa
     5581 gtacttccgt aaggtatggc cagtactgac gtttgccttt accagccgtt ccagtgctgc
     5641 gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg ggtgttcctg aatccatcgc
     5701 cagtttcgcc gcctctttcg gtgcaaccat tggtcagaac ggctgtgccg gtttgtatcc
     5761 ggcgatgctg gcggtgatgg ttgcgcctac ggtgggcatt aacccgatgg acccgatgtg
     5821 gattgcgacg ctggtcggta ttgttaccgt tagttccgca ggcgttgccg gtgtcggtgg
     5881 tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg ggcctgccgg taacgctggt
     5941 ggcattgtta atctccgttg aaccgcttat cgacatgggt cgtacggcgc taaacgttag
     6001 tggctcgatg accgctggca cgctgaccag ccagtggctg aagcaaaccg ataaagccat
     6061 tctggatagc gaagacgacg ccgaactggc acaccgttaa tcctgatttg ttacgttaaa
     6121 cgcctgtcat cccgacaggt gtttttttat tcttcgacct cattttcctg acgaatctga
     6181 tttgcccaac gctgggcgga ttcaggcaca gtaaacgccg gtgagcgcag aaatgactcc
     6241 cccatcagta caaacgcaac atatttgcca cgcagcatcc agacatcacg aaacgaatcc
     6301 atctttatcg catgttctgg cggcgcgggt tccgtgcgtg ggacatagct aataatctgg
     6361 cggttttgct gacggagcgg tttcttcatt actggcttca ctaaacgcat attaaaaatc
     6421 agaaaaactg tagtttagcc gatttagccc ctgtacgtcc cgctttgcgt gtatttcata
     6481 acaccgtttc cagaatagtt tccgaagcgg gatctggctg gtggtctata gttagagggt
     6541 tttttgacca aaacagcggc cctttcagta ataatttaag gagacgagtt caatgtcgca
     6601 acataacgaa aagaacccac atcagcacca gtcaccacta cacgattcca gcgaagcgaa
     6661 accggggatg gactcactgg cacctgaaga cggatctcat cgtccagcgg ctgaaccaac
     6721 accgcctggt gcacaaccta ccgccccagg gagcctgaaa gcccctgata cgcgtaacga
     6781 aaaacttaat tctctggaag acgtacgcaa aggcagtgaa aattatgcgc tgaccactaa
     6841 tcagggcgtg cgcatcgccg acgatcaaaa ctcactgcgt gccggtagcc gtggtccaac
     6901 tctgctggaa gattttattc tgcgcgagaa aatcactcac tttgaccatg agcgcatccc
     6961 ggaacgtatt gttcatgcac gcggatcagc agctcacggt tatttccagc catataaaag
     7021 cttaagcgat atcaccaaag cggatttcct ctcagatacg aacaaaatca ccccagtatt
     7081 tgttaggttc tctaccgttc agggcggcgc tggctctgcc gataccgtac gtgatatccg
     7141 tggctttgcc accaagttct atactgaaga gggtattttt gacctcgttg gcaataacac
     7201 gccaatcttc tttatccagg atgcacataa attccccgat tttgttcatg cggtaaaacc
     7261 agaaccgcac tgggcaattc cacaagggca aagcgcccac gatactttct gggattatgt
     7321 ttctctgcaa cctgaaactc tgcacaacgt gatgtgggca atgtcggatc gcggtatccc
     7381 gcgcagttac cgcactatgg aaggcttcgg tattcatacc ttccgcctga ttaatgccga
     7441 agggaaggca acgttcgtac gtttccactg gaaaccactg gcaggtaaag cctcactcgt
     7501 ttgggatgaa gcacaaaaat taaccggacg tgacccggac ttccaccgcc gcgagttgtg
     7561 ggaagcgatt gaagcaggcg attttcctga atacgaactg ggcttccagt tgattcctga
     7621 agaagacgaa ttcaagttcg acttcgatct tctcgatcca accaaactta tcccggaaga
     7681 attggtgcct gttcagcgtg tcggcaaaat ggtgctcaat cgtaatccgg ataacttctt
     7741 tgctgaaaac gaacaggtgg ctttccatcc agggcatatt gtccccggtc tggatttcac
     7801 caacgacccg ttattgcagg ggcgtttgtt ctcttatacc gatacacaaa tcagtcgtct
     7861 tggtgggccg aatttccatg aaattccgat taaccgcccg acctgccctt accataattt
     7921 ccagcgtgac ggcatgcatc gtatggggat cgacactaac ccggcgaatt acgaaccgaa
     7981 ctcgatcaac gataactggc cgcgcgaaac accgccgggg ccgaaacgcg gcggttttga
     8041 atcataccag gaacgcgtgg aaggaaataa agttcgcgag cgcagtccat cgtttggcga
     8101 atattattcc catccgcgtc tgttctggct aagtcagacg ccattcgagc agcgccatat
     8161 tgtcgatggt ttcagttttg agttaagcaa agtagttcgt ccgtatattc gtgagcgcgt
     8221 tgttgaccaa ctggcgcata ttgatctcac tctggcccag gcggtggcga aaaatctcgg
     8281 tatcgaactg actgatgacc agttgaatat caccccgcct ccggacgtca acggtctgaa
     8341 aaaggatcca tccttaagtc tgtacgccat tcctgacggt gatgtgaaag gtcgcgtggt
     8401 agcaattttg cttaatgatg aagtgagatc ggcagacctt ctggccattc tcaaggcact
     8461 gaaggccaaa ggcgttcatg ccaaactgct ctactcccga atgggggaag tgactgcgga
     8521 tgacggtacg gtgttgccta tagctgctac ctttgccggt gcgccttcgc tgacggtcga
     8581 tgcagtcatt gtcccttgcg gcaatattgc ggatatcgct aacaacggcg atgccaacta
     8641 ctatctgatg gaagcctaca aacaccttaa accgattgcg ctggcaggag acgcgcgcaa
     8701 gtttaaagca acaatcaagg tcgctgacca gggtgaagaa gggattgcgg aagctgacag
     8761 cgccgatggt agttttatgg atgaactgtt aacgctgatg acagcacacc gcgtgtggtc
     8821 acgcattcct aagattgaca aaatcccggc gtaaatatgg ataaggtcac gccccctggg
     8881 gagtgacctt accaccttac ttacacatca tgataactac ccaaccgata accacgctca
     8941 gcaatcgcat atttcaacga cgctgaagtc agcacatgca gttccgttaa ccgagggaag
     9001 caataagcac tctggcgaat cgtattatcg acaaacgccg gatggcacat gacttccaac
     9061 gagcgttccc cacgatggct ggaatcatca agcacttgca ggaacaatgc ctcgctaatc
     9121 tcttcaccat aaaacgcgct actgaatccc tgcgaactgc gcagattagc cggtaaatca
     9181 ccatcgttag atagcggttg acggtcgatg cgcaacgcaa tcccctcttc agccacaaac
     9241 tttgccacaa tcgggaaaat ctgcgggaac atatgcacat gatgatggct atcaagatgc
     9301 gtaggtttgc gtccaaatag ctcaatgaaa cgcaaatact gactggcgag ctcctgagta
     9361 atttcttcca gcggtaaagc atcttcttct gccaactgcc agatccattt tcccagcaca
     9421 ccatcgcggg ttaaccccgg catagctgtc agaggcttgc ccatagtaag gacaaagtgc
     9481 atccctatgg ccagactcgg ttcatcacgg ctcaactgca ccgcatggtc aatagcctgc
     9541 ccattcacca gcgccgtcgt cgacgtgaca atcccattgc gacaggcctc gataatgccg
     9601 tagttctgac ctttgcttaa gccaaaatca tcggcattaa caatcagtaa gcgttccata
     9661 atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc agtcggcaaa atttggcagc
     9721 catttctcgt gcgccagaat catctcgcgt gccagcagct cagcatcgcg atcggaatgc
     9781 accaacggac taaggtttag cgccagtaag acatcgttaa attctccact aagtgcggcg
     9841 ttgctggcag caatctcgaa gcctttaatg gtgtgaatca gccccatcac tttatcatcg
     9901 aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc ccagcgtaca ggtcatttct
     9961 accgcccagt ctgccggaat attatcaata tgcccatgat gcgggatatt aacgtaatgt
    10021 tcagcttgct tgtcgttgta gatagcgttg atcacttcgc acgctgcatc agagtaataa
    10081 gccccaccgc gctgttccag ttctttcggc ttaacgttca actccgggtt tttatacagc
    10141 tcaaaaagtt gtttctcgac tttctgtact acctgcgctc gtgcgccgcc tttgtagtat
    10201 tcgcccattt caatagccag catctctttt tgcttgaagt aataaagcaa ataagaacac
    10261 ggcaacagat tcagagaacg aattaagcct tcgctaaatg gcagatcgaa aatattttta
    10321 acgccagatg cttttaactg cccggatgcc acaccatcaa gcaattcggc aaagcgtgat
    10381 ttgccattta ccagcacatc cttaatgaac accatatggt tgaggccgaa cagatcgata
    10441 gataaatcat cgcagtcttt cagcatcaga acatcgcgaa taaacatctt catgccgatc
    10501 ggaatattac acacgccgat aaagcgttta aatccggtat gacgataaac ggcttcagtg
    10561 accattccag ccgggttagt gaagttaatc acccatgcat tcggacatag ttcttcgaca
    10621 tcttttacga tgtcaaaaat caccggaatg gtacgcagac ctttaaacag accgcccgcg
    10681 ccgttagttt cctgaccaag ataaccatga cttaatggaa tacgttcatc cagttcccgc
    10741 gccggtaatt ggccaacgcg cagttgggta gtaacgaaat cagcatcttt caatgcttcg
    10801 cggcgatcca gcgttttata aagcttcatc gggacgccag cgttatcaat catccgttgg
    10861 cagagatcaa aaataatatc cagtttcgct ttaccacctt cgacatccac cagccataat
    10921 tcgctgaccg gcaattcgtg ataacgctta ataaatcctt ccagtaactc cggggtatag
    10981 ctgctcccgc caccaatagt gacgactttt aatttctggc tcataatttc tcccttcagt
    11041 acagaatact gatatctggc atatctgccc ccggacataa ataatccagc aacaggacag
    11101 atatgtgaat tgtcaggtat aacgacttac tgattaaatt cagtcaattt cttacgatag
    11161 ctcttaggcg taaaggatgt taatttctta aacgttttaa taaacaagct cgggctacta
    11221 taaccggcct caaacgcaat atccgtcact gaatagttgg tcatttccag ttgttttttg
    11281 gcaaaattaa tacggatttc attaataatc tgcattggcg ttttaccata gtatcgttga
    11341 gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg tcaccatatt ctccagcgcc
    11401 gattcactaa actgctcttt atcatgcatc ttttctaccg tacttttcag ccactgcggt
    11461 atatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac ggttaatgac ataaaaagta
    11521 accatctcaa caaactcttc cagccctgtt tcgcggaaat tcaatgaaga aatcactgtt
    11581 tccacatagg tgagaaaagc gttattggtc cggtatacct gcgaaactac aaagcaataa
    11641 ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac tgatcccaac gttcaatata
    11701 cgcgtggcac caaattcata aaaactttga tggtgcgaac ctaacggaat aaaaacaaaa
    11761 tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct ggaaataacg cccggttaat
    11821 accagagtaa attcataata gtcgtgctga tgcagtccgc tgatactctc agttttattg
    11881 tagataaaca catggaagtt tttgccatta aacaactgct gttctcgggc agtggcaatt
    11941 tccggcgcgt taatcactgg ctgcatcatc gactccttat gccttcagtt tttcatgaag
    12001 ctcaattaat tcagtaatca gttcacgcgc aagcatggac gtcattaaat gatcctgagc
    12061 gtggaccagc accagactca ctttcatctt accttcgcca gcatcgcctt caatcagttt
    12121 cgtctgtacc agatgcgctt cattcaatgc catccgtgac tgatccatca tcgcttttgc
    12181 tgcggcaaaa tcgccctgct tcgcctgttt cagtgccgca tacgccaggc tacgcgcttg
    12241 tccggagttg atgataagcc ccatcaccac ttcttccagc tcttcagctt ccgtttgcgt
    12301 atcgggaata ttatcgagat ccatcataca tcgttcctct ctattcttac cggcacgatt
    12361 acccgtaccg gcatcaatta aaatttcagg gcgttggcga tatcttcttc gctctcttct
    12421 ttatcaatcg cgttttgtgc tttgttagcg accacaacaa agggcagata aattaacgtt
    12481 gcgataccaa gattgaagag tgcgaccagc aatgcggcga cgctaccgtt ggtgttaaag
    12541 aaggctccca gaccggtggg catggtccac ggtgcaatat tggtcaccgg tggaataatg
    12601 cccatgtagt acgctgccag ggtgattgcc gccagaatcg gttgtaccag tacaaacggg
    12661 ataaacatca ccgggttcat gataattggc agaccaaaca gaatcggttc gttaatctgg
    12721 aagatgccgg acggcagcgc cagttttgcc acctgacgat aatcagcacg gcgagaagcg
    12781 ataaagatag ccaggatcag gcctaaagtc gcaccactgc caccaaggaa aataaaggag
    12841 tccagcatcg gcttggccca gatatggaag gtcttaccgg ctgccagcgc cgcttcgacg
    12901 gaaccatatt gctgataggt cgcgatattt tccagcgccc acggcgtcat aatgccgttg
    12961 tccagtgcgg tcagcgccag cgcgccatga ataccgaaga accagagcag tggaacaaag
    13021 atcacatagg cccagcctac cacgctaccc aacgatgcca gtggggttga gatggtatcc
    13081 ataatgatct gatggaagtt ggtgccccag gtattcaacg cccaggcaat aatccccatc
    13141 acggaaagaa taataaaacc gggaattaat gctgagaaag agcgcgaaac cgatgtcgga
    13201 acgctatcag gtaacttaat gacccagtta cgatgaacaa taaaggtaaa catttctgcc
    13261 accaccaggc caataataat cccggagatg atattcgccc cacctaacca gtttgcgcca
    13321 accgcatagg cctcaccgac actatatggg gtgacggtca taaatgctgc aacggataac
    13381 aacccggccg ccagcgcatc gactttacgc tcttctgcca gcgccatgcc aataaagaac
    13441 ggggccatta aagacattat ccctaatgtt ccgttatata cgttgccgcc aatacctttc
    13501 agaccattaa gtgtttcaat ggttgaggca tccaggcgaa tacctaagga ataaaaaaac
    13561 gacccctccc caaagcttag aaaaacgttg ttaattaata caaacatggc ccccgcaagg
    13621 gttaacggca ttaagcgaat aaagccattt ttgattgcat taacgtgtgg ctgctttcct
    13681 attttaactg caaaagggag gagtaccttt tcaagcgatg caataacatt actcatagaa
    13741 aaataccctt aaaaaccgca atttaaatat tgcggtattg atttatgaaa taactctttg
    13801 acgggaaaat ttaaaataaa ttaatttgct gcggcttttt taatcgctgc aaccgcagcc
    13861 ttaagcacgc ctaaaccatc gactttgcca taaagcagcg agtcaattac ttcaaccggt
    13921 ttgttgggta acaaacgctg gatttcgggc aacatataag caatctgcgg ccctaataac
    13981 acgacatcgg cattctgacc tttttcacca gccagtgttt ccggaaatgc ttcaataatg
    14041 accggaactt catatttttc tgcctgtgcg cgcatttttg ataccagtaa agaggtagac
    14101 atgcccgcag aacaaaacag ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg
    14161 attatctgtc agccagacac tccgcaagcc ttaacctgct tccatgctct gggtaacttg
    14221 cgaaaccaac atgacttttt ttgtggctga aaggagtata cggtataggc ctgagcaata
    14281 gtatttcttt gaccttataa attgtctctc cttgacctgt ggttatgacc ctcttcacat
    14341 ttcgggcaaa taattcgcga agataattaa gaacacaggc caaaaaaaac cggcgcaatg
    14401 gccggtttcc gttgttactc aagctttcag gcgaagtgat tacttcgtag cctgtggatc
    14461 agtgtcgtat tcagcacagg tctgataacc ggagttgatg acatgtccgg tatcatctaa
    14521 cgcgacaaag taggtttctg ctttaccatc acgttgaccc aggatgtagg tctggcaagt
    14581 accacgagca tggatcatgc tcacttcaga cgaaggttta cccgcaattt gtgcaacctg
    14641 cgcccggctc atgccttttt tgacgtcttt caccacaggc tgtacaaact ggtctttggt
    14701 acgatcataa gccgtacaac ccgccagcat ggttaatacc gccgctgcac tcagaattcc
    14761 tgccatattc ttgttcatat tccgtcctct tgtttatcag cgtgttagat aagcctggaa
    14821 tacattgggc gctttttcaa gcccgtgaac gaaacggctc cgctttcaga ggattcctgt
    14881 atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc aacgggttag
    14941 ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa tgacattgca acaacaaata
    15001 ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag aggaaattcg tcgtagtatc
    15061 gattttctga aaagctacct gcaaacttat ccgttcatta aatcactggt gctcgggatc
    15121 agcggcggtc aggattccac gcttgccggt aagctgtgcc agatggcgat taatgagctg
    15181 cgccaggaaa ccggcaacga atcactgcaa tttattgccg tacgcctgcc ctatggtgtt
    15241 caggccgacg aacaagattg ccaggatgcc atcgccttta ttcaaccgga tcgcgtatta
    15301 accgttaata tcaagggcgc ggtactcgcc agcgagcagg cgctgcgcga agcgggcatt
    15361 gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat
    15421 agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt
    15481 accggattct tcactaaata tggtgacggc ggtacggaca ttaatccgct gtatcgtctc
    15541 aacaaacgtc agggtaaaca gttactggcg gcattaggtt gcccggaaca cctttataag
    15601 aaagcgccaa cggccgatct tgaagatgat cgcccttctc taccagatga agtggcactc
    15661 ggcgtgacct atgacaatat cgacgactat ctggaaggga aaaacgtacc tgaacaggtc
    15721 gccagaacaa tagagaactg gtatctgaaa accgaacata aacgccgtcc gccaattacc
    15781 gttttcgatg atttctggaa aaagtaataa tttgcacata ttggtttgtg cgaaaaagag
    15841 tactttgttc acgccggatg cggcgtgaac gccttactcg acctacaaaa ctatgcaaat
    15901 tcaatacatt gcaggagtcg aataggcctg acaggcgtag cacgtcaaac ggtgtaacct
    15961 ttgtcatcga cccgcctctt ttttaatcgc tccccgcctg ttacactgga tagataacca
    16021 gcattcggag tcaacagtgg tacggcgttt aacttctccg cggctcgaat ttgaagctgc
    16081 ggcaatttat gaatatcccg aacatttacg ttcattcctt aatgacttac ccacccgacc
    16141 tggggtgtat ctgtttcatg gcgaaagtga caccatgccg ctctatatcg gcaaaagcat
    16201 taacatccgc agccgcgtcc tttctcattt acgtaccccg gatgaagccg ccatgctacg
    16261 gcaatcccga cgcatcagct ggatatgtac cgccggtgaa atcggcgctc tgctccttga
    16321 agcgcgatta atcaaagaac aacagccact gtttaataaa cgcttgcggc gcaatcgcca
    16381 gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga
    16441 tttttcacga gcccccaacc tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc
    16501 attacagacc atcgctgatg aacaaaaact ttgttatggc ctgttgggac tggagccgtt
    16561 aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg
    16621 taaagagagc catgacgatc atgcgctacg tttgcgccag tctctggaac gtttacgagt
    16681 cgtttgttgg ccgtggaaag gtgcggtagc gctgaaagaa cagcacccgg aaatgactca
    16741 atatcatatt attcaaaact ggctgtggct gggggcggtt aattcgctga aagaagcgac
    16801 aacgttaatt cgggcacccg ccgggtttga tcacgacggt tataaaattc tttgtaagcc
    16861 gctgctttcc ggtaactatg aaattactga acttgatccg gtgaatgacc agcaagccag
    16921 ttgatttcac tgaacaacag tttaccttgc ggttgcaaaa gccgcagggt gtgcctgcca
    16981 tcgaaccagc atgcgccctg gtcggtagtg agcagtttgt cgcccaattg ccacgccccg
    17041 tttattacaa acaccacccc gccgcgtgaa ccaaaggtag taaacgtacg ttcggcaatt
    17101 ctgactttcg ccttacagac atccaggcgt gtcatgatgt tgaaatccat cgacatctgc
    17161 cctgccgtca gtttcgcttt taccaccagg tccgctgcaa aggaaaaagg ttgtaacggt
    17221 tttaaggtat ggttaaagcg gtctgcgctt tcaaggaaca tctcgccgcc tcccagcaac
    17281 gtcactatcc tttccatacc gggaaacaaa gaaaactccc cattcgccgc gatggatgta
    17341 atgctggcac gccagtaaaa atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt
    17401 tcaccggcag cgtttcgcca cagattcacc gacattttac gcatatcaaa gtattccata
    17461 cccgctcccg gcccgttctt ttcattgtta tcgttcaggt aaccgtggat ggttatcggc
    17521 aactcgggtg atttacgtta gtggcgatca ggcttttttt gcatctgttt gagaagagaa
    17581 aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc
    17641 ttacgttttt tggctgaaag attattcagc agttgcaggc attttacctt ttgccgctgg
    17701 acgttctgtc agacgcttct caaaattagc attaaattgc tttttctgtt ccggcgtcag
    17761 gatgttgtaa atcttgttct gggtttccat gtgcgccagc atgttagctt tgcgctgttc
    17821 ttccattttt gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc tggcaatgat
    17881 gtcatgcatt gcgcggcgtt cttccagcgg cggacgtttc atctggtcac gctggccttt
    17941 catgatttcg cggatctgct gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat
    18001 gtcctcatgc ggaccgaact tgcctttatg gtgcatcatc ggcttcgcgt cagccggtgc
    18061 tgcggtagtg gtgtctgcgg catgggccag gttagccgcg ccaagagcca gggtagaggc
    18121 aacaaacagt gcagttaatt tacgcatatt ctatatcctt cctttcagtt atttattacg
    18181 gctttcttaa gtagcgtgcc gtgttgacga gattaacttt actgacttta gcgtcaatta
    18241 atcagagcaa cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa
    18301 aaaaagaaaa tttgatggag agtgatgaaa gaatattaca acacgatgat tttgcagaga
    18361 ttatgaagaa ctataccgga tgactggtga taaataaagc aaataaccag gattaatctg
    18421 tattaattta taagaaagca acttaatacc cgcagaatga tttctgcggg taagtattag
    18481 cttatttttt cgagcattaa tcccgcacgt aatcccaacg ctaccaacgg attagggaat
    18541 aacacatact ctacatcatg ggttacggta aaacgttcct ccccgtcctg cgccagcaat
    18601 gttcctttct caaacggcat aaaattcagc gtgtcatttg ccatatgcat ttcgaaggac
    18661 ggcgagtggc gagtaatttg cgaaaccacc cgataacgga gcggcggtgt tctcacgata
    18721 ccgacactct caccagatag cagcgcagca attgcgctgg cagttactgc aaactggcga
    18781 agatcgtttt gcccaaaggg caacgctttg ccaagttcca gcgtacaggc cagcgcgcca
    18841 aaatgtctgg cgctgaaatg ggtaaacgta ccaccaggct cctgatggaa caccagcgcc
    18901 tccagccccg ccgcacccag ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc
    18961 ggtaatacac cgaactgcgg atgcaaggag ccacgaattg cggtatgtag atcaaggtgc
    19021 cagcgcacag attctttgcc ctggtcataa aaatcttcca ggcactgctc cagttcgcgc
    19081 gcccgacagg tttccccgct ttcagcaaac agttgccaac gaccgccaaa cattcgattc
    19141 atatcgctat ggcaataacg tttcccttgc ttcagcgcag gaggattccc gaggatcacc
    19201 agtaaccgcc aacgtaacgg gatctcaccg tgagatatcg cgccaagcaa cgcgtccagc
    19261 atttccacag gtgccgtctc attaccgtgg atccccgctg aaatcaccag cgcgccttgc
    19321 ggtggcgtta atggcgtcag ttccagcaca ccatcgccca gccagcgcca gcgaacgccg
    19381 ttgatttctc gctcggtgat aaccggtttt ttacccgtta aggttagagc aagaaaatta
    19441 tccattgccc cctccctcgc gctggaatgg ataaaccgaa ccaagatcca ataattgagt
    19501 cagtgtatcc agcgcttccc gtccttcgcg cagcaattgt ggatcggcaa gatccgccgc
    19561 agtaagacga tcgcggtagt aacgatccac ccagtcattg agcgcattaa acagcgtatc
    19621 gttcatcatc accgccggat tcaccgcccg gcgttcttct tctgtcaaca ccacgcgcaa
    19681 ccgcaggcac gccgggccac cgccattcgc catgctttca cgtaaatcaa aaacttttag
    19741 tgagcttatt gggttatcgg cagcaagcaa ttcattgaga taacgccata ctccggcgtg
    19801 ttcccgacac tcctgcggca gcaccaacac catggaacca tcatcgcggc tcagcagttg
    19861 gctgttaaac agataagtag acaccgcatc tgacacggaa acctgcgccg tgggcacctc
    19921 tattgccata aaaccactga cccgtgcacg cagatttgcc agtaactgtg actggcgagc
    19981 gaacgcctgt tgatggcaaa acagcacctg gcggttactc acggcaatca cgtcattatg
    20041 aaaaacgccc tggtcgataa cgtccgggtt ttgctgggcg aaaatcacct gttggggatt
    20101 cacctgattc aaccttgcca ccgcctcgct ggcttcacgg gtctgccgcg ccggataacg
    20161 ggaaggccgg gtatcattgc cctcttctcg cccgtagaca aaaagttgca tacccggttc
    20221 accgtaatga ccgccgagac gattgtggtt tgccgccccc tcatcaccga gcaaggctac
    20281 ctgcggcaac gccgaatgga cgctaaattt ctcttcgtcg ttaaaaatcg cttttaacag
    20341 cgattcggta acgggggctt ccagcgaacg gtgaaattta ttgttcaggt tagcaacagt
    20401 gagatgcact ttgccatcca gcgtatcggc ggatggcgcg atcgttgccg cattggctac
    20461 ccacattggc gaagcggaac tgacgctgga aagccagtgc ggtgcctggc gtgcaacttt
    20521 ttccagtacc tgctcatcgc taccactgaa tcccaactga cgcagcaccg gaataaacgg
    20581 acgctcgtgc ggcgggatca cggcctgggg gaatcccgca ccggcaagtt ttttcatttt
    20641 cagtaagccc tgttttgccg ccagtcgcgg gttagacacc tgaaaacggt gacgggtaga
    20701 ggcttcatta ccaaacgaca ggcccgcgta atggtgcgtc agccctacca gcccgtcgaa
    20761 attgacttcc caggcgttca tcgcaccacc tcatcggaaa aatccagccc ggggttaagc
    20821 gttgcgggca acgttaacga gtccgactcc agacttgcca tcggccaggc acaataatct
    20881 gcggcatacc aggcgctggg gcgatgattg ccggaagcgc caatgccgcc gaatggcgcg
    20941 gtactggcag caccggtaag cggtttgttc cagttaacaa tccccgcccg tgcctccagc
    21001 aacagttgat cgaatttttc ccgctcgggg gaaaccagac cgcaagagag gccgaagcga
    21061 gtgttattcg ccattcgaat cgcttcatcg aaattgtcat aacgccagac gcgcaataac
    21121 ggcccaaaca cttcttcatc cggcgagcca gtaacgcctg tcatttcaat gatccccggc
    21181 gtcagcaacg atgtccctgc ttgtaataag cgcggcgcaa gcagggtccg tccgcccatc
    21241 gcctccagtt cctgccaggc agtaaccacc tgctgtgcgg cctgctcaga aatcagccca
    21301 ccaataaatg gctgcggttc gtcatcccaa gtgcccggcg ttaaccgctg gctgacggca
    21361 accaggcgag caagaaatgc atcgccctgc gttccacttt tcagaaataa acggcgggcg
    21421 caggtgcagc gttgcccggc agtgacaaac gccgactgaa tggtcagatg gacagcggca
    21481 tcgatatccg ccacctcatc gataattagc gggttattac cgcccatctc aagggcgaga
    21541 attttctccg gctgaccgga aagctggcga tgcagctggt agccggtatt ggcgctaccg
    21601 gtaaacagca aaccgtcgag atcctccagc gcactcagcg cctgacccgt ttcacgcccg
    21661 ccctgcacca ggttcagcac gcccggcggc aagccagcct gctgccataa acgcattacc
    21721 gcttcgccgc tccacggtgt cagttcgctg ggtttaaaga taatggtgtt acctgccagc
    21781 aatgccggaa cgatatgtcc gttcggtaaa tgaccgggga aattatacgg cccaaacacc
    21841 gccagcacgc cgtgcgggcg atgtcgcagg ctcgccgcgc cgtccggcat ttcgctacgc
    21901 tgctcaccgg tacgaacgtg atacgcctta attgatatcg cgattttatt gatcatcgcc
    21961 gtcacttcgg ttgccgcttc ccagcgcggc ttaccggttt ctctggcaat aatcgcggtt
    22021 aattcggctt tattgctttc cagcaatccg gcaaagcgtt caactcttac ctgacgatcg
    22081 ccaaatgaga gccgagccca gcgcggaaac gccgcacggg cagcccgaca agcctgcccg
    22141 acctgagcgg catcggcatc attgccctgc cataacacct cgcccgatac cggattacgc
    22201 ttcacacgca atgcgccctg gcccgttacc cagtcaccgt tgatccataa agtcatgctg
    22261 ttttctcctc tgcgcacagg cgcaccagac gaacgcgatc ccctgcgtgg catttgaggg
    22321 catccagttg tgcggcggtt aaaatcaaac gctcggtcgc tggatcggta cgcaccagca
    22381 ccacgcggaa atggtgataa ttttcattgg cgaccaggca ggccggaaaa tcgccctgcg
    22441 caggctgccc ttcggccact tccaccagcc gacttttacg gatggcgcgc acgcggtcga
    22501 tatcacattc aagtgtcggc ccgccgtcaa agatgtcgat atagttacgg tagcgaaaac
    22561 cttctttctc cagcaccgca cgggcaggcg cggtttgcgg atgtacctga ccgatgacgt
    22621 cctgggcttc ctgggataaa aagtgggtat agatcggatg tttcggcatc agttctgcaa
    22681 taaatgcctt ttgcccggtg ccgcagagaa aatcagcgcg gctaaaatcc atcgaaaaga
    22741 agcgtttacc gaggctttgc cagaacggtg aatagccgtg ttcgtcaatc accccgcgca
    22801 tttcggcgac cactttgtca ttaaacttgt cgcgaaaagc cgccataaac ataaagcgcg
    22861 atttcgacag caaatagccg ttgccctctt tgcgccactc cgggtcgaga aacagcgtgc
    22921 acagctcgct gctgccggtg tgatcgttac tgagaaacag cgtcggcaat gcgttataga
    22981 cattcagctc tttcgaagcg tgaaccagcg tgccgacacg atagttgtac cagggatcgt
    23041 tcagcccaac cgccacctca atggcgcaaa tccccgccac agtgcctgtc tcaccatctt
    23101 ccagtacaaa cacataaccc tgctcacttt tgggcagttc gccttgccag gttttgattg
    23161 ccctttcaat acgcaccgaa agcgtggctt cattggcggg aagcgacgtc aggccgccgc
    23221 ccgttttgct ggcaagctgc atcagcgccg agacatctga gcgctcaacg ggacgaatga
    23281 ccatcatgat gaacctcggc taacaaagtg ttcgcaagca gctgcaaagc gatccagtcc
    23341 ggtcgtcacc tcttcttcgc tgacattgag cgctggcgca aaacgcacca cgttgccacc
    23401 cgcaatcagc accatcacgc ctgctttcgc cgcttcctga gagagctgtt tcgcttgccc
    23461 ggcgtaatcg gcattcaaca cgcaaccaat caacaaacct aagccgcgaa cttcactgaa
    23521 caaactgtag tggtgattaa tggaatttag acgctcaaca aaccagtcgt gacgctgttt
    23581 aaccccgtta agcatctctg gcgtgttgat gagatccagc actttgcctg ccaccgccga
    23641 ggccagcgga ttgccgccat aggtggtgcc gtgagtgcca acggtcatca cgctggcgca
    23701 ctctttggtt gccaacaacg caccaaccgg gaaaccgccg cccagcgctt tggcggtagt
    23761 taacagatca ggcgtcacgc cgtagtgcat ataggcatac agttccccgg tgcgcccgac
    23821 accggtttgt acttcatcaa agatcagcaa cgcattgtgg cggtcacaca attcacgcag
    23881 accttgtaaa aatgcgttgc tggctggcac cacaccgcct tccccctgga tgggttcgac
    23941 aatcaccgca caggtggcgt cgtcaatcag cgcactggca gagttaatat cgttatatgc
    24001 agcatgacga atatccggcg gcagtggcgc aaaatcctgt gaataggctg gctgcccacc
    24061 cgcactgaca gtaaacagcg tgcgaccatg aaacgcattt ttgaacgcca cgatgccgct
    24121 cttatggctg ccgtagcggt cgtgagcgaa tttacgcgcc agttttagcg ccgcttcgtt
    24181 ggcttccgca ccggagttac aaaagaagac gcgatcggca aacgtggcgt cgatcaattt
    24241 tttcgccagt cgcagtaccg gctcgttggt gtaaccgttg ccggtatgcc agaacttact
    24301 cgcctgttcg ttcagcgcct cacgcagttc cggatgcgca tggcccagcg cgttcaccgc
    24361 aatgccaccc gcgaagtcga tatactcttt cccctgctga tcccacaagc gcgaaccttc
    24421 gccacgtacc ggtataaacg gtgccggagc gtaaacaggt atcatccatt catcaaagtt
    24481 ttcacgcgta attggctgag acatagcgac ctctacagta aataattcgt tatttatatg
    24541 ttaataataa gtaatgtttg cgctgtaaat gtagattgca gggttcgtgc cagccagtga
    24601 taaaagtgca taaacggcgg aggctaactg gaaatcaagc agttataacc acatcatatg
    24661 catttaaagt gcatataaag tgaatgcgtt tgcgatgtgg gtgaataaaa agaataaaaa
    24721 acgcaatgtt atgcagaagt aaaatataat tctggaattg tgatcattga cgaaatttac
    24781 tggaaattac tgcgccattc tgacgcaacg cgcaccaaaa gcgggcattt tttgcgccat
    24841 cgttgacatc attaacaacc atcgatcaaa tcacttaaca acaggcggta agcaaagcga
    24901 aattctgcta ccatccacgc actctttatc tgaataaatg gcagcgacta tgaaatttgt
    24961 ctcttttaat atcaacggcc tgcgcgccag acctcaccag cttgaagcca tcgtcgaaaa
    25021 gcaccaaccg gatgtgattg gcctgcagga gacaaaagtt catgacgata tgtttccgct
    25081 cgaagaggtg gcgaagctcg gctacaacgt gttttatcac gggcagaaag gccattatgg
    25141 cgtggcgctg ctgaccaaag agacgccgat tgccgtgcgt cgcggctttc ctggtgacga
    25201 cgaagaggcg caacggcgga ttattatggc ggaaatcccc tcgccgctgg gcaatgtcac
    25261 cgtgatcaac ggttacttcc cgcagggtga aagccgcgac catccgataa aattcccggc
    25321 aaaggcgcag ttttatcaga atctgcaaaa ctacctggaa accgaactca agcgtgaaaa
    25381 tccggtgctg attatgggcg atatgaatat tagtcctggc gacctagata ttggcattgg
    25441 cgaagagaac cgtaagcgct ggctgcgtac cggaaaatgt tctttccttc cggaggaacg
    25501 cgaatggatg gagaggctga tgagctgggg gttggtcgat accttccgcc atgcgaatcc
    25561 gcaaacagca gatcgtttct catggtttga ttaccgctca aaaggttttg acgataatcg
    25621 cggtctgcgc atcgacctgc tgctcgccag ccaaccgctg gcagaatgtt gcgtagaaac
    25681 cggcatcgac tatgaaattc gcagcatgga aaaaccgtcc gatcacgccc ccgtctgggc
    25741 gaccttccgc cgctaattta gctgttctcc tggctcaaac tgggtcagga gaattaacct
    25801 tgagaaaaat caacaaactg tcagtaatga tttgttgcct gccgcccttt gttataccgt
    25861 cactgcgttt ttagttgtct gaccacttct ctattatcaa gtttgatata ggaaactcca
    25921 cgatgaacgc tgagcgtaaa tttctttttg cctgtcttat ttttgcgctg ctcatttacg
    25981 ctatccacgc tttcggttta ttcgatctgc tcaccgattt acctcactta caaacactca
    26041 tccgccagag cggattgttc ggttatagcc tctatatttt gttattcatc attgccaccc
    26101 tctttctgtt accaggaagc atactggtga tcgctggagg aatagttttt ggcccgttct
    26161 tagggacact actctcatta attgccgcca cgctggcctc ctcgtgctca ttcctgctgg
    26221 cgcgctggct ggggcgtgat ttactgctga aatatgtggg ccatagccat acttttaagg
    26281 ccattgaaaa aggcattgcg cgtaacggta ttgattttct tattctgacc cgcttaatcc
    26341 cgttgtttcc ttacaatatt caaaattacg cttacggatt aaccacaatc gccttctggc
    26401 cttataccct tatttcagca ctcacgactc tgcccggtat tgttatttat accgtgatgg
    26461 caagcgatct cgccaatgaa ggcattacgc tgcgctttat tttacaactc tgtctggcgg
    26521 gcctggcgct gtttattctc gtccagctcg caaaactcta cgctcgacac aaacatgtgg
    26581 atctgtctgc ttctcgccgc agcccactta ctcacccaaa aaatgaagga tagaacgatg
    26641 ttgcaacatt attcagtgtc atggaaaaaa ggactgactg cactctgctt actggctgtt
    26701 gctgggctta gcggctgcga tcaaaaagag aatgcggcgg caaaagtgga atacgacgga
    26761 ctttcgaaca gccaaccgct gcgtgtcgat gccaataacc atacggtaac catgctggtg
    26821 caaattaatg gtcgtttcct caccgacgac actcgtcacg gtattgtgtt taaagatggc
    26881 tccaacggac ataaatcgct gtttatgggt tatgcgaccc cgaaagcatt ttatgaagcc
    26941 ctgaaagagg caggtggtac gccgggcgaa aacatgacga tggataataa agaaacgact
    27001 catgtcacag gcagcaaact ggatatttcg gtcaactggc aaggggcggc aaaagcgtat
    27061 tccttcgatg aagtgattgt tgacagtaat ggcaagaaac tggacatgcg ctttggcggt
    27121 aatttaacgg cagcagaaga gaagaaaacg ggttgcctgg tgtgtctgga tagctgcccg
    27181 gtcggcatcg tcagcaatgc aacatacact tatggtgcgg tggaaaaacg cggcgaagtg
    27241 aaattcaaag gcaatgcctc agttctcccg gcggataaca cgctggcaac ggttaccttt
    27301 aaaatcaccg aataaagcag gataaaggat gatgaagatg caatcgcgta aaatctggta
    27361 ctaccgtata accctcatca tcctgttgtt cgctgtgctg ctggcatggg cgctgcttcc
    27421 cggcgtccat gagtttatca atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg
    27481 tatagaacgc tttattcagt cttacggcgc actagcggca gttgtctcgt tcttgttgat
    27541 gattttacag gccattgctg caccgctacc tgcgtttttg atcacctttg ccaatgcgtc
    27601 gctgtttggt gcgttctggg gcggcttact gtcgtggacc agttcgatgg ccggcgcggc
    27661 actgtgcttt tttatcgcca gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa
    27721 aaccgtactt gacagtatgg acggcttttt cactcgctac ggcaaacaca ccatcctggt
    27781 atgtcggtta ttgccttttg tccctttcga tccaatcagc tatgctgccg gtttgacttc
    27841 aatacgtttt cgctcgtttt ttatcgccac cgggcttggt cagttaccgg caactattgt
    27901 ttattcctgg gcgggcagca tgttaacagg cggtactttc tggtttgtca ccggactgtt
    27961 tattctgttt gccctgaccg tggtgatttt tatggcgaag aaaatatggc ttgagcgcca
    28021 gaagaggaat gcctgatggg tttaccgccg cttagcaaaa ttcctttcat tttacgtccg
    28081 caggcgtggc tgcatcgtcg ccattacggc gaggtgctaa gccccattcg ctggtggggg
    28141 cggatcccgt ttatctttta tctggtgtcg atgtttgtcg gctggctgga gcgcaaacgc
    28201 tcaccgctcg atccggtagt acgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg
    28261 tgtgagtttt gcgtggatat caccagtatg aaagtcgccg agcgtaccgg cagcaccgat
    28321 aaactgctgg cagtggctga ctggcggcaa agcccgctct ttagcgatga agaacggctg
    28381 gcgctggagt acgccgaagc cgcaagcgta acgccgccaa cggtcgatga tgccctgcgc
    28441 gcccgactgg ctacgcattt tgacgctcag gcgctcaccg aactgacggc attgataggc
    28501 ctgcaaaatc tgtcagcccg ttttaattct gccatggaca ttcccgctca ggggctgtgc
    28561 cgtattcctg aaaaacgttc ttaaggagag aagatgcgcc attgtgggtg gttgctggga
    28621 ttgttatcgc tgttttcttt ggcaacacat gccagtgact ggcaggaaat aaaaaatgag
    28681 gccaaaggac aaaccgtctg gtttaacgcc tggggcggcg ataccgcaat taaccgctat
    28741 ctcgactggg ttagcggcga gatgaaaacc cattacgcta taaacctgaa gattgttcgt
    28801 ctcgcggatg ccgcagacgc ggtgaagcgc attcagaccg aagctgctgc cggacgcaaa
    28861 acgggcggtt cggtggatct gctctgggtg aacggcgaaa acttccgcac cttaaaagag
    28921 gccaatttat tacaaacggg ctgggcggag actctgccca actggcgcta tgtcgacaca
    28981 cagcttccgg tgcgggaaga tttttcagtg cctacagaag gggctgaatc gccctggggc
    29041 ggcgcacaac tgacatttat cgcccgccgc gatgttacgc cacagccacc acaaacgccg
    29101 caaaccttac tggagtttgc taaagccaat cccggcacgg ttacctatcc gcgcccaccg
    29161 gactttaccg gcacggcgtt tcttgaacag ttgctgatta tgctaacgcc agatcccaca
    29221 gcattaaaag aagcgccgga cgatgcgact ttcgcccgcg tcactgcgcc cttgtggcaa
    29281 tatcttgatg cactgcatcc gtatttatgg cgcgaaggaa aggatttccc gccttcgccc
    29341 gcgcggatgg atgctctgct gaaagctaac acattgcgcc tgtcgctgac ctttaaccct
    29401 gcacatgcgc agcaaaaaat cgccagcggc gatttgcctg caagcagtta cagttttggt
    29461 tttcgcgagg ggatgattgg caacgtccat ttcgtcacca ttcctgccaa cgcgaatgcc
    29521 agtgctgcgg cgaaggtagt tgccaatttc ctgctctcac ccgatgcgca actgcgtaaa
    29581 gcagatcccg ctgtctgggg cgatccttct gttctcgatc cgcaaaaact gcctgacggg
    29641 cagcgcgaaa cattgcaatc gagaatgccg caggatctgc cgccggtact ggctgaaccg
    29701 cacgcaggtt gggtaaatgc gctggaacaa gaatggctac accgttacgg tacgcattaa
    29761 tctttttgct gtgggcgatg atggcggtga tttatgcacc gctgatcccg gcagccctca
    29821 cgctgatatc gcctgctttg tcgttgacgc actggcaggc gttatttgcc gatccacagt
    29881 taccgcaggc attactggcg acgctggtgt cgacaaccat tgcggcggtc ggggcgttgt
    29941 tgattgccct gctggtgatt gtggcgttgt ggccagggcc gaaatggcag cgtatgtgcg
    30001 cccgtctgcc gtggctgctc gccattcccc atgtggcttt tgccaccagc gcccttctgc
    30061 tctttgctga cggagggctg ctttatgact atttcccgta tttcactccg ccaatggaca
    30121 aattgggtat tgggctgggc ctcaccctgg cggtgaaaga aagcgcattt ctgctgtgga
    30181 tcttagcggc agtattgagc gaaaaacggc tgttgcagca actcattgtg ctggattcgc
    30241 tgggctacag ccgctggcaa tgcctgaact ggctgctgtt gccctccgtc gcgtctgcgc
    30301 tggcaatggc gatgctggcg attgtcgcct ggtcgctgtc ggttgtggat gtggcgatta
    30361 ttcttgggcc aggtaatccc ccgacgctgg cggtaattag ctggcagtgg ttaactcagg
    30421 gcgacgccga tcaacaaacg aaaggcgcac tcgccagcct gctgctgata ctgttactcg
    30481 ccgcctacgt tttgctgggc tatctgctat ggcgtggctg gcgacgtact attccccgtg
    30541 tagatggcgt tcgtaagcct gccacgcctt tattgccagg caatacgctg gcgagttttt
    30601 tacccttaac cggtgtgctg tgtgtggttc tgctggcgat cctcgcggat cagtcgacga
    30661 tcaatagtga agcgctcatc aacagcctga caatggggct ggtggcggca ttcatcgctt
    30721 tgctcctgtt actgctgtgg caggaatggg ggccacagcg tcgccagttg tggctatggt
    30781 tgcccatttt attacccgct ctgcctctgg tggcgggcca gtacacgctg gcgctatggc
    30841 tgaacctgga tggaagctgg acggcggtgg tctgggggca tctgctgtgg gtaatgccgt
    30901 ggatgctgtt tatcctgcaa cccgcctggc agcgcattga ttcacggtta attttgattg
    30961 cgcaaacact gggctggtcg cgggccaaaa tcttctttta cgtaaaatgc ccactcatgt
    31021 tgcgccctgc gctgattgcc ttcgcggtgg gattttcagt cagtattgcg cagtatatgc
    31081 cgacgctgtg gctgggcgcg gggcgttttc cgacgctcac cactgaagcg gtggcattaa
    31141 gcagcggcgg cagcaacggt attctcgccg cccaggcttt atggcaactg ctgttaccgc
    31201 ttattatttt tgccctgacc gcgttagtcg caaaatgggt aggttatgtc agacaaggac
    31261 tccgctaatg ctctgcgtga aaaatgtttc gctacgttta ccagaaagcc gcttgctgac
    31321 aaacgttaac ttttcagtag ataaaggtga cattgtcacg ttaatgggac catccggctg
    31381 cgggaaatcc actctgtttt cgtggatgat tggtgcgctg gccggacagt tttcttgtac
    31441 aggggagcta tggcttaatg agcaacggat tgacatgcta cccaccgcac agcgtcagat
    31501 tggcattctt ttccaggatg cactgttatt tgaccagttc agcgtcgggc aaaatttact
    31561 gctggcgcta ccggcgacac ttaaggggaa tgcccgacgt aatgccgtga atgatgcact
    31621 tgagcgttca ggccttgacg gagcgttcca tcaggatcct gccactttgt ctggcggtca
    31681 gcgagcgcgc gttgctctgc tgcgcgccct tctcgctcag ccaaaagcgt tgctactgga
    31741 tgagccattc agccgtcttg atgtggccct gcgcgataat tttcgccatt gggtgttcag
    31801 cgaaattcgc gccatggcga tccctgttgt gcaggtgacg cacgatcttc aggatgttcc
    31861 tcctgatagc cccgttctgg atatggcgca gtggtcagaa aattacaaca aactgcgata
    31921 acgcaaagtt tttcccaatg cgtcagttca gaatggcgca ctcaaaacta caatgtcggg
    31981 attttcgatg aaacgtgttt ctcaaatgac cgcgctggca atggctttag ggctggcttg
    32041 cgcttcttcg tgggccgctg aactggcgaa gcctcttaca cttgaccagc ttcaacaaca
    32101 aaatggcaaa gcgattgata ctcgccccag cgcgttttat aacggctggc cacaaacctt
    32161 aaatggccct tccggtcatg aacctgccgc cttaaacctc tctgctagct ggctcgacaa
    32221 aatgagcacc gaacagctca acgagtggat caagcaacat aacctgaaag ccgatgcccc
    32281 ggtggcgctg tacggtaatg acaaagatgt cgacgccgtc aaaacgcgac tgcaaaaagc
    32341 aggttttacg catatctcca tccttagtga cgcgctaagc gaaccttccc gtctgcaaaa
    32401 actgccgcac tttgaacagc tggtatatcc gcagtggctg catgacctgc aacaaggtaa
    32461 agaggttacg gcgaaacctg ccggtgactg gaaagtcatt gaagcggcct ggggcgctcc
    32521 aaaattttac cttatcagcc atattcccgg cgctgactac atcgacacca acgaagtgga
    32581 aagcgaaccg ctgtggaaca aagtttctga tgaacaactg aaagcgatgc tggcaaaaca
    32641 cggcattcgc catgacacca cggtcattct gtacgggcgt gacgtatacg ctgcagcgcg
    32701 tgtggcgcaa attatgcttt atgccggcgt gaaagatgtg cgcctgctgg acggaggctg
    32761 gcaaacctgg tccgacgcgg gtctgcccgt tgagcgcgga acgccaccga aagtgaaagc
    32821 ggaaccggat ttcggcgtga agatcccggc acaaccgcaa ctgatgcttg atatggaaca
    32881 agcgcgtgga ctgctgcatc gccaggatgc atcgctggtg agcattcgtt cgtggccaga
    32941 atttatcggt acgaccagcg gttacagcta tattaaacca aaaggtgaaa tagccggagc
    33001 acgttgggga cacgcaggta gcgactcgac gcatatggaa gatttccata acccggatgg
    33061 caccatgcgc agcgccgatg atataaccgc tatgtggaaa gcatggaata tcaaaccaga
    33121 tcagcaagtt tcattctact gcggcactgg ctggcgcgcg tcagaaacct ttatgtacgc
    33181 acgcgcaatg ggctggaata acgtctccgt ttacgacggt ggctggtacg aatggagcag
    33241 cgatccaaaa aatccggtag caaccggtga acgcggcccg gacagcagta aataacattg
    33301 caattactga cgctggagcg acttcagcgt cagataccca ctccacaccc gcgtaaaggt
    33361 cgtcatccag cacaacgcac caaataccca cgcaaaccac ggaaaacagg ccggaaagag
    33421 gcaactcaga acaaacagca aaatcgtctc gctgccttcg gttaacccgc ccagataata
    33481 aaacgattta tgcgcataac caggattatc aatctgatgt ttcgccgcca gcgcagcaaa
    33541 agcgagaaaa ctgctgcccg tgccgataaa cgcaaacagc agccagccgc ctaccagggc
    33601 gttttgctcc ggtgcggcga ggataaaccc aaaaggcacc agcgcgtaaa aaagaaaatc
    33661 gagagaaata tcgagaaagc cgcccgcgtc ggtaagccct ctgcgccgcg ccagcgcacc
    33721 gtccagacca tcaagcagcc tgttcaacaa aatggcgact agtgccgcca gataccagcc
    33781 cagcgccaga aaaggcagtg ccagcacgcc gatggcaaat cccactaatg ttaaaccatc
    33841 tggcgtaatg cccggtttat caagaacccg cacgcactga tgcaacaacg gtttaatccg
    33901 gggatgaaga tggcggtcta gcacaggcac tccttaaata taaagccttt ctgattgagc
    33961 aacagtgcgg atattatggc atttttcgct tatctgcccg tgtgtaattt atgaaaatga
    34021 ttgaagttgt tgccgccatc atcgaacgtg atggcaaaat tttactcgcg caacgccctg
    34081 cccatagcga tcaggcggga ttatgggagt ttgccggtgg taaagtcgag ccggatgaaa
    34141 gccagcggca ggcgctggtg cgtgagttaa acgaagaact gggcatcgaa gcaactgttg
    34201 gtgactatgt tgccagccat cagcgagaag tttcggggcg gattatccat cttcatgcct
    34261 ggcacgtacc cgacttccac gggacgttac aggcacatga acatcaggcg ctggtctggt
    34321 gctcacctga agaggcgctg cgatatccgc tggcccctgc tgacattcca ttattagagg
    34381 cgtttatggc ttcacgcgcc gccagaccag cggattagtg cttagggttt tgtcatcccg
    34441 ttggcattgc agcagtattc cttcggcttt aattactgcc ccttcagaat aattttgatc
    34501 ctgataaacg cagcactgag tacagggctg cgctgactgc ccgcctgaac taaacacttc
    34561 tggcggtacg tttacctcca cgtccggatg ataatgcggg ttagccagtg taattaatgg
    34621 aaatgctaat actacggcga tcaatgctcg actcacaggg aactccttaa cgttattgtc
    34681 tcttctacag ataacggtag ccgggcggca aaactttagc gtctgagtta tcgcatttgg
    34741 ttatgagatt actctcgtta ttaatttgct ttcctgggtc atttttttct tgcttaccgt
    34801 cacattcttg atggtatagt cgaaaactgc aaaagcacat gacataaaca acataagcat
    34861 aatcgtatta atatataagg gttttatatc tatggatcag acatattctc tggagtcatt
    34921 cctcaaccat gtccaaaagc gcgacccgaa tcaaaccgag ttcgcgcaag ccgttcgtga
    34981 agtaatgacc acactctggc cttttcttga acaaaatccc acatatcgtc agatgtcatt
    35041 actggagcgt ctggttgaac cggagcgcgt gatccaattt cgcgtagtat gggttgatga
    35101 tcgcaaccag gtgcaggtca accgtgcatg gcgtgtgcag ttcagctctg ccatcggccc
    35161 gtacaaaggc ggtatgcgct tccatccgtc agttaacctc tccattctca aattcctcgg
    35221 cttcgaacaa accttcaaaa atgccctgac tactctaccg atgggcggtg gtaaaggcgg
    35281 cagcgacttt gatccgaaag ggaaaagcga aggcgaagtt atgcgtttct gccaggcgct
    35341 gatgactgaa ctgtatcgcc acctgggcgc ggatactgac gttccggcag gtgatatcgg
    35401 cgttggtggt cgtgaagtcg gctttatggc ggggatgatg aaaaagctct ccaacaatac
    35461 cgcctgcgtc ttcaccggta agggcctttc atttggcggc agtcttattc gcccggaagc
    35521 taccggctac ggtctggttt atttcacaga agcaatgcta aaacgccacg gtatgggttt
    35581 tgaaggaatg cgcgtttccg tttctggctc cggcaacgtc gcccagtacg ctatcgaaaa
    35641 agcgatggaa tttggtgctc gagtgatcac tgcgtcagac tccagcggca ctgtagttga
    35701 tgaaagcgga ttcacgaaag aaaaactggc gcgtcttatc gaaatcaaag ccagccgcga
    35761 tggtcgagtg gcagattacg ccaaagaatt tggtctggtc tatctcgaag gccaacaacc
    35821 gtggtctgta ccggttgata tcgccctgcc ttgcgcaacc cagaacgaac tggatgttga
    35881 cgccgcgcat cagcttatcg ccaatggcgt taaagccgtc gccgaagggg caaatatgcc
    35941 gaccaccatc gaagcgactg aactgttcca gcaggcaggc gtactgtttg caccgggtaa
    36001 agcggctaat gctggcggtg tagcaacatc gggcctggaa atggcgcaaa acgctgcgcg
    36061 cctgggctgg aaagccgaga aagttgacgc acgtttgcat cacatcatgc tggatatcca
    36121 ccatgcctgt gttgagcatg gtggtgaagg tgagcaaacc aactacgtgc agggcgcgaa
    36181 cattgccggt tttgtgaagg ttgcggatgc gatgttaagc cagggcgtga tttaatcgtt
    36241 attacaatcg tctggttttc cttataacca gacgattttc cctgagttaa ttaaatcctt
    36301 agcagacgtt tgcaatttgc agccgtttgt tctgcatcct gaatatcctg ttccgacatt
    36361 aagcatcgca acgcatcttt ttcaatccag gggttgttat ttccggcaat tgttagccac
    36421 atccatgctt gtttattatc aattttagtg cccgtcccct gagcatacat tgccgcagtc
    36481 tggtgctgcg cttcggtcgc ccctaaacgc gccgcttttt cgtaccagtg cagcgcatga
    36541 ttttcatctt ttgcgatata ttttgtaccg tacagataca tttcagctaa caaaaatatc
    36601 gcctctattc gaccacgctg cgctgcacca gtaagccatt gagtagcctc tgcgttattt
    36661 tcaggatcat cattgttggt taaataatat ttccccaatt catacatggc atcccctgcc
    36721 ccatctgccc cattttttgc tgctttggta aaccagtaca gcgctttttc ctggtcctgt
    36781 ggaatactct cacttccgct atacaacctg gcagtatgat attgcgcatg taaatatccc
    36841 tgttctgctg attgcaaaaa ccaacgatgg gcctgaacct catcgcgctc cacgccaatc
    36901 ccttgtatat acatttgtcc caactcatac tgggcaacct catgtccttg tgtggcggca
    36961 gataaatacc actgcaatgc ttcttcataa tttcgtaatg gctcaacagc aaagcgtgag
    37021 atacgggcaa gctcatattg cgcagatgtg ttgccatttt gggcagcaag taaaaaccat
    37081 tttcgtgcct cacaaatatt cttatccgtg ccccttccat cgctatacat gatacccagc
    37141 gcatacagag catcgataac attatgctgc gcggcgcggg taaaccagtt aaaggctaat
    37201 tttagatcct gtgaaacctc atcaccttcg taattcattt tgccaagaga ataccaggct
    37261 agtgaatagc cttgctctgc agcatgtaag caggactggt atacagaaga tggataataa
    37321 aaaagacctt ttaattttat ggcatt
//
LOCUS       DAZKHK010000036        37203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKHK010000036 DAZKHK010000000
VERSION     DAZKHK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.036"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..544
                     /locus_tag="ACHOS5_001842"
     CDS             <1..544
                     /locus_tag="ACHOS5_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9776663.1"
                     /translation="SMFATALLSGVAFNALADDANQGSGKITFKGEVIDAPCSIAPGD
                     EDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSSASVDATD
                     SNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNFKARMESL
                     GRTATPGNVQAQANYVLDYK"
     gene            631..3153
                     /locus_tag="ACHOS5_001843"
     CDS             631..3153
                     /locus_tag="ACHOS5_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9776664.1"
                     /translation="MNNKNTFSRDKLSHAIKNALSGVVCSLLFVLPVHAVEFNVDMID
                     AEDRENIDISRFEKKGYIPPGRYLVRVQINKNMLPQTLILEWVKADNESGSLLCLTKE
                     NLTNFGLNTEFIESLQNIAGSECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQQQNSGNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYGDNAR
                     MMVDTDGISGIEINSNRTVTNGLGIAVIPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSTLHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3169..3909
                     /locus_tag="ACHOS5_001844"
     CDS             3169..3909
                     /locus_tag="ACHOS5_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9776665.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRDYISTLPPMERIEPDEQIQIRLMALASLNDLPQD
                     RETLFYYNVREIPPRAKEQNVMQIAMQSRLKLFWRPKAIELKEGEMIPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVAPFTSATQHLSSLPSTFQLGFVAD
                     YGGLEMFKVECNSIQSLCQSSPAKKGKI"
     gene            3906..4955
                     /locus_tag="ACHOS5_001845"
     CDS             3906..4955
                     /locus_tag="ACHOS5_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFP9776666.1"
                     /translation="MITLFRLLAILCLFFNVSAFAVDCYQDGYRGTTLINGDLPAFKI
                     PENAQPGQKIWESGDINITVYCDNAPGWTTDNPSENIYAWIKLPQINSADMLNNPYLT
                     FGVTYNGVDYEGINERIDTHACLDKYEQYYNGYYYDPVCNGSTLQKNVTFNAHFRVYV
                     KFKSRPAGDQTVNFGIVNVLQFDGEGGANMSSNAKNLRYVITGLDNISFLDCSVDVRI
                     SPESQIVNFGQIAANSIATFPPKAAFSVSTIKDIASDCTEQFDVATSFFTSDTLYDNT
                     HLEIGNGLLMRITDQKTQEDIKFNQFKLFSTYIPGQSAAMATRDYQAELTQKPGEPLV
                     YGPFQKDLIVKINYH"
     gene            complement(5009..5662)
                     /gene="ribB"
                     /locus_tag="ACHOS5_001846"
     CDS             complement(5009..5662)
                     /gene="ribB"
                     /locus_tag="ACHOS5_001846"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP9776667.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5766..5914)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6036..6335
                     /gene="ubiK"
                     /locus_tag="ACHOS5_001847"
     CDS             6036..6335
                     /gene="ubiK"
                     /locus_tag="ACHOS5_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP9776668.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPIAELQSPPAIPPVDKAE"
     gene            complement(6370..6570)
                     /gene="glgS"
                     /locus_tag="ACHOS5_001848"
     CDS             complement(6370..6570)
                     /gene="glgS"
                     /locus_tag="ACHOS5_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9776669.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            6840..7469
                     /gene="yqiJ"
                     /locus_tag="ACHOS5_001849"
     CDS             6840..7469
                     /gene="yqiJ"
                     /locus_tag="ACHOS5_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9776670.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7496..9157
                     /gene="yqiK"
                     /locus_tag="ACHOS5_001850"
     CDS             7496..9157
                     /gene="yqiK"
                     /locus_tag="ACHOS5_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9776671.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9397..9456
                     /locus_tag="ACHOS5_001851"
     CDS             9397..9456
                     /locus_tag="ACHOS5_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9776672.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(9576..11009)
                     /gene="hldE"
                     /locus_tag="ACHOS5_001852"
     CDS             complement(9576..11009)
                     /gene="hldE"
                     /locus_tag="ACHOS5_001852"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP9776673.1"
                     /translation="MKVTLPEFECAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11057..13897)
                     /gene="glnE"
                     /locus_tag="ACHOS5_001853"
     CDS             complement(11057..13897)
                     /gene="glnE"
                     /locus_tag="ACHOS5_001853"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP9776674.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPKWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRALLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLNEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(13920..15221)
                     /gene="ygiF"
                     /locus_tag="ACHOS5_001854"
     CDS             complement(13920..15221)
                     /gene="ygiF"
                     /locus_tag="ACHOS5_001854"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP9776675.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLDGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKVGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKI
                     SDSFKRFADIHLSRYAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            complement(15291..15743)
                     /gene="tnpA"
                     /locus_tag="ACHOS5_001855"
     CDS             complement(15291..15743)
                     /gene="tnpA"
                     /locus_tag="ACHOS5_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEc41 family
                     transposase"
                     /protein_id="HFP9776676.1"
                     /translation="MWLFRMYNISTFFWEGQYVCEINRSRYSAYLLHVHVVFMTKYRR
                     PVFGELHHANMRQYVAEVCADFGAELKECDGEADHVHMLIEYPPLVQLTKLVNSLKSV
                     TSRRLRNDFIDLRAAYSKPVLWSRSYFIGSCGGAPLEVVKKYIQNQRG"
     gene            15756..16961
                     /locus_tag="ACHOS5_001856"
     CDS             15756..16961
                     /locus_tag="ACHOS5_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9776677.1"
                     /translation="MLIQKAYKYRLYPTDQQAQRLRQLCGCARFVWNYALNETLSIHD
                     AGGKIPSAFDLNKRLTGWKKLPELAFLSEGYTDNLQQKLKDLRSAWDRCFDKSLTAEK
                     PVFKKKTKGCDSIRFVNFSKYCGLDYGRVKLPSGLGWVKFRQSRKIEGVIKNCTISQH
                     AGHWYVSFQVELAVTDPIHASTSAIGLDAGITKLATLSDGTVFEPVNSFKKNQDKLAR
                     LQRRLARMVKFSANWKKQKAKISRFHSHIANIRRDYLHKTTTTISKNHAMIVIEDLKV
                     SNMSKSAAGTVDQPGRNVAAKSGLNRAILDQGWAEMRRQLEYKQAWRGGDVLAINPAY
                     TSQKCACCGHTSKNNRRTQASFICTACGYTANADVNGARNILTAGFEVMAAGQILPSV
                     RKGRARKAA"
     gene            17304..17924
                     /gene="ygiM"
                     /locus_tag="ACHOS5_001857"
     CDS             17304..17924
                     /gene="ygiM"
                     /locus_tag="ACHOS5_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP9776678.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            17988..19226
                     /gene="cca"
                     /locus_tag="ACHOS5_001858"
     CDS             17988..19226
                     /gene="cca"
                     /locus_tag="ACHOS5_001858"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP9776679.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQND
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(19414..20235)
                     /gene="bacA"
                     /locus_tag="ACHOS5_001859"
     CDS             complement(19414..20235)
                     /gene="bacA"
                     /locus_tag="ACHOS5_001859"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9776680.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGYT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(20326..20694)
                     /gene="folB"
                     /locus_tag="ACHOS5_001860"
     CDS             complement(20326..20694)
                     /gene="folB"
                     /locus_tag="ACHOS5_001860"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP9776681.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            20799..21416
                     /gene="plsY"
                     /locus_tag="ACHOS5_001861"
     CDS             20799..21416
                     /gene="plsY"
                     /locus_tag="ACHOS5_001861"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9776682.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIRTKFKRKREKDPE"
     gene            complement(21429..22361)
                     /gene="ttdR"
                     /locus_tag="ACHOS5_001862"
                     /gene_synonym="dan"
     CDS             complement(21429..22361)
                     /gene="ttdR"
                     /locus_tag="ACHOS5_001862"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP9776683.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            22568..23479
                     /gene="ttdA"
                     /locus_tag="ACHOS5_001863"
     CDS             22568..23479
                     /gene="ttdA"
                     /locus_tag="ACHOS5_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP9776684.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            23476..24081
                     /gene="ttdB"
                     /locus_tag="ACHOS5_001864"
     CDS             23476..24081
                     /gene="ttdB"
                     /locus_tag="ACHOS5_001864"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP9776685.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            24130..25593
                     /gene="ttdT"
                     /locus_tag="ACHOS5_001865"
     CDS             24130..25593
                     /gene="ttdT"
                     /locus_tag="ACHOS5_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP9776686.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     RAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(25636..26649)
                     /gene="tsaD"
                     /locus_tag="ACHOS5_001866"
     CDS             complement(25636..26649)
                     /gene="tsaD"
                     /locus_tag="ACHOS5_001866"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9776687.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            26887..27102
                     /gene="rpsU"
                     /locus_tag="ACHOS5_001867"
     CDS             26887..27102
                     /gene="rpsU"
                     /locus_tag="ACHOS5_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9776688.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            27213..28958
                     /gene="dnaG"
                     /locus_tag="ACHOS5_001868"
     CDS             27213..28958
                     /gene="dnaG"
                     /locus_tag="ACHOS5_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9776689.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVAMSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            29153..30994
                     /gene="rpoD"
                     /locus_tag="ACHOS5_001869"
     CDS             29153..30994
                     /gene="rpoD"
                     /locus_tag="ACHOS5_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9776690.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(31072..31578)
                     /gene="mug"
                     /locus_tag="ACHOS5_001870"
     CDS             complement(31072..31578)
                     /gene="mug"
                     /locus_tag="ACHOS5_001870"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP9776691.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            31703..31778
                     /locus_tag="ACHOS5_001871"
     tRNA            31703..31778
                     /locus_tag="ACHOS5_001871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31736..31738,aa:Ile,seq:cat)
     gene            complement(32584..32868)
                     /locus_tag="ACHOS5_001872"
     CDS             complement(32584..32868)
                     /locus_tag="ACHOS5_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776692.1"
                     /translation="MTETEALEIIRSITGISQATGKQEATQPDSVTAENYARVVAEVM
                     RRDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKKPERLRW"
     gene            complement(33410..33793)
                     /locus_tag="ACHOS5_001873"
     CDS             complement(33410..33793)
                     /locus_tag="ACHOS5_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9776693.1"
                     /translation="MQNHYNDLKPIAEMMYPDPAVEELKAIADKMRLSERLVDMNQVM
                     ELTTLSRRTLLNLEARGEFPERVQVTEGRKAWYLSEVIDWINNIPRASEYCRVPVPKK
                     PDAALCLKIDRVRRNARDGRYKLIG"
     gene            complement(33805..34146)
                     /locus_tag="ACHOS5_001874"
     CDS             complement(33805..34146)
                     /locus_tag="ACHOS5_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9776694.1"
                     /translation="MAIHYTELMAGTEALVTTLGIFSANKGVIPAFTPLMQEDTTGAL
                     VVWDGTSAGKAVYVSAVQLDTAKKTQAQVYKTGVLNVDALNWPESVKELSVKVAAFVG
                     SGISVQPLARV"
     gene            complement(34157..35197)
                     /locus_tag="ACHOS5_001875"
     CDS             complement(34157..35197)
                     /locus_tag="ACHOS5_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9776695.1"
                     /translation="MVDLYSPAQLVQVANAEDVQKKLNVLFTSLFFTRSVMFESRDII
                     LDTIDDPNIPIAAFCSPMVGSKVSRDEGYESKTIRPGYMKPKSSIDPNKLAVRPAGVS
                     PEQYNAFGARNIKVKQAIVNQAKAIRTRIEWLAVQAITTGKNIIEGDGIERYELDWNI
                     KPQNIITQSGGTEWSGKDKETFDPNDDIESYAEFSEGVTNIIIMGGNVWKKYRSFRAI
                     KEALDTRRGSNSELETALKDLGDSVSFKGYMGDVAIVVYSGRYTDEDGTEKYFLDPDL
                     MVLGNTALQGIVAYGGIQDPELIRMGLTKAELAPKNYIVPGDPAIEYVQTHSAPQPIP
                     ARINRFVTVRIG"
     gene            complement(35415..35837)
                     /locus_tag="ACHOS5_001876"
     CDS             complement(35415..35837)
                     /locus_tag="ACHOS5_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9776696.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQSGNDDPYGDNIPF"
     gene            complement(35834..36079)
                     /locus_tag="ACHOS5_001877"
     CDS             complement(35834..36079)
                     /locus_tag="ACHOS5_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776697.1"
                     /translation="MTAKRTKKSQSHALDLTEHWLRGSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            complement(36367..>37203)
                     /locus_tag="ACHOS5_001878"
     CDS             complement(36367..>37203)
                     /locus_tag="ACHOS5_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP9776698.1"
                     /translation="GGSGKSVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLI
                     IMPDMTRYAGDGAGIKAITGGDKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGG
                     ISRRRVIFNFSEVVPENERDPMLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKS
                     EEALAIKREGDSLVDFCGYLMASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGF
                     GKPVTLTRFGKDMPGAMAEYGREYIKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDS
                     KN"
BASE COUNT         9375 a         9877 c         8975 g         8976 t
ORIGIN      
        1 atctatgttc gcaaccgcat tgttatcagg cgttgctttt aacgctcttg ctgacgatgc
       61 taatcagggt tcaggtaaaa ttacttttaa aggtgaagtt atcgatgcac cttgttctat
      121 tgctcctggt gatgaagatc agacaataaa cctcggtgaa gttgctgata ccgtattaaa
      181 aagcggtcag aaatcactgc ctgtagatgt caccattcat ttgcaggatt gtattttatc
      241 tgacggcact aacactgttg ataaagtcaa aatcaccttt agttctgcca gtgttgacgc
      301 taccgactcc aacctgctta aaaacactct ggaaggtaac atcggcggcg caactgatgt
      361 aggcgtacgt ctggtgaaat cagacaacac caacgtgact cttggcactc caatcactat
      421 caacttcccg acgactaact cttaccagga gttgaacttt aaagcccgta tggagtctct
      481 gggacgcacc gcgaccccgg gtaacgtgca ggcacaggct aattacgtac tcgactacaa
      541 gtaatcggta gtcaatacct acacaacgcc aggcggtaaa gccgatgaaa tttcatcggc
      601 aactttgggc ctttttagaa atggattttt atgaacaata aaaacacgtt ttcccgggat
      661 aagttatccc atgcaattaa aaatgccctg tctggcgttg tgtgttccct actcttcgtt
      721 ttgccagtcc acgccgtaga attcaacgtc gatatgattg acgcagaaga ccgtgagaat
      781 atcgacatct ctcgttttga gaaaaaaggc tatatccccc ctggtagata cctcgttcgt
      841 gtgcaaataa ataaaaatat gttgccacaa acgttaatac tggaatgggt aaaagccgat
      901 aatgaaagtg gttcgttact ctgcttaacc aaagaaaatt tgactaattt cggtcttaat
      961 acggaattta ttgaatcatt gcaaaacata gctggcagcg aatgtctcga tttaagccaa
     1021 cgtcaggagt taacgacacg acttgataaa gctacgatga tattatcgct aagtgttccc
     1081 caggcatggt taaaatacca ggcaacaaac tggacgccac cagagttttg ggataccggt
     1141 atcgccgggt ttatccttga ttacaacgtg tacgccagcc agtatgcccc acatcacgga
     1201 gacagcaccc aaaacgtcag ctcctatggt acgttaggct ttaacctcgg cgcatggcgc
     1261 ttacgtagcg attaccaata taatcagaat tttgctgatg gacgctcggt aaaccgcgac
     1321 agcgaatttg cgcgaactta tctgtttcgc cctatcccct cctggtcgtc aaaattcact
     1381 atgggccagt acgacctgag ctccaatctt tacgatacct tccactttac tggcgcatcg
     1441 ctggaaagtg atgaaagcat gctgccgcca gatttacagg gttatgcgcc acaaattacc
     1501 ggcatcgcgc agaccaacgc gaaagtaact gtggcacaaa atggtcgtgt actttatcaa
     1561 accactgtcg cgccaggccc ttttactatt tctgatttgg ggcaatcgtt tcaggggcag
     1621 ctggatgtca cagtggaaga agaagatggc cgcaccagca ccttccaggt tggctccgca
     1681 tccattccct atttaacccg taaagggcaa gtgcgctata aaacgtcact gggaaaaccg
     1741 acatccgtcg ggcataacga tatcaataat ccctttttct ggacggcgga agcctcctgg
     1801 ggctggctga acaatgtgtc gttgtatggt ggtggcatgt tcaccgctga tgattatcag
     1861 gctatcacta ccggtattgg ctttaacctt aaccaattcg gttcgctttc ttttgatgtc
     1921 accggagcag acgcgtcttt acagcaacaa aatagcggca atctgcgtgg ttacagctat
     1981 cgcttcaact atgcaaagca tttcgaatcg acaggcagtc agattacctt cgcgggttat
     2041 cgcttctcag ataaagatta cgtgtcgatg agtgagtacc tcagctcgcg taatggcgat
     2101 gagtcaatcg ataatgaaaa agagagttat gtcatttcct tgaaccagta ctttgaaacg
     2161 ctggaattaa actcttatct caacgttaca cgcaatactt attgggacag cgccagcaat
     2221 accaactact ccgtatctgt aagcaaaaac tttgatattg gcgatttcaa aggtatatct
     2281 gcatcgctgg cagtaagtcg aatccgctgg gatgacgacg aagagaatca atattacttc
     2341 tctttctctc tacctttaca acaaaaccgc aacatctcct acagtatgca gcgaacggga
     2401 agcagtaata cttcgcagat gatttcctgg tacgattcat cagatcgcaa caatatctgg
     2461 aatatttcag cgtcggcaac ggacgacaat atacgtgatg gcgaaccaac actgcgcggc
     2521 agctaccagc actattcgcc gtggggacgt ctgaacatta atggcagtgt acagccgaat
     2581 cagtacaatt ctgttaccgc aggctggtac ggttcactta ccgctacacg tcatggtgtc
     2641 gcccttcacg attatagcta tggcgataac gcccgcatga tggtcgatac cgatggcatc
     2701 tccggcattg aaatcaactc taaccgtacc gttaccaacg ggctgggcat cgccgtgata
     2761 ccttcgttat cgaactacac cacctccatg ttgcgggtga acaataacga tctgccagaa
     2821 ggtgtcgatg tcgaaaactc ggttattcgc accacgctca cccagggtgc catcggctac
     2881 gcaaaactga atgccaccac cggttaccaa atcgtcggcg ttattcgtca ggaaaatggg
     2941 cgcttccctc cactgggtgt gaatgtcacg gataaagcga caggtaaaga tgtgggcctg
     3001 gtcgcggaag acggcttcgt ttatctcagc ggtattcagg aaaatagtac tctgcattta
     3061 acctggggtg ataatacctg tgaagtcacg ccgccaaacc aaagtaacat tagcgaaagc
     3121 gcaataattt taccttgtaa aacagtcaaa taattcagga tgaatataat gaaatcacca
     3181 ttaatcacaa caggaatgat ttgtttactg gggatctgca actttgccca ggcgactgtc
     3241 tcccccgaca gaacacgcat catttttaat gcttcgaata aaagcgcaac ggttcgcctg
     3301 accaaccaaa gtaaaatcga tccctatctg gctcagtcgt ggattgaaga tgccagcggt
     3361 aaaaaaacgc gtgattacat ctcgacattg ccaccgatgg aacgtattga gcctgatgaa
     3421 cagatacaaa ttcgcctgat ggcactggcc tcgttaaacg acttaccaca ggaccgagaa
     3481 acgttgttct actataacgt gcgagaaatt ccgccacgcg ccaaagaaca gaatgtaatg
     3541 caaatcgcca tgcaaagccg cctgaagcta ttctggcggc caaaagcgat tgagttaaaa
     3601 gagggggaga tgattcctct gcaaaaagta acgattaccc gcaccgccgc cggtctgacc
     3661 ctgaataacc cgacgcctta tcatattacc gtcggttata tcggcactaa tggcaagaca
     3721 ctgatgcccg gtgcggacag tattatggtg gcaccattta cttctgcaac tcaacaccta
     3781 agcagcctgc cctctacctt ccagttaggc tttgtggcgg actacggcgg gctggagatg
     3841 tttaaagtcg aatgtaattc catccaatcc ctctgccaaa gctctcctgc taagaagggg
     3901 aaaatatgat tacgcttttt cgtttactgg cgattctttg cctgtttttt aacgtttcag
     3961 cttttgctgt tgattgctat caggatgggt acagaggaac aaccctcata aatggagatt
     4021 taccagcgtt caaaattcca gagaacgcgc aacctgggca aaaaatttgg gagagtggag
     4081 atattaatat cacggtttat tgtgacaatg caccaggatg gacaacagac aatccatcag
     4141 aaaatatcta tgcctggatc aaattacccc aaataaatag tgccgacatg ttgaataatc
     4201 catatttaac ctttggcgtg acttataatg gtgtagatta tgaagggata aatgaaagaa
     4261 ttgatactca tgcatgcctg gataaatatg agcaatacta taatgggtat tattatgacc
     4321 ctgtatgcaa tggcagtacc cttcaaaaaa atgtaacatt taatgcccat tttcgcgtct
     4381 atgtaaaatt caaaagccgc ccagcaggag atcagacggt gaactttggc atagtcaacg
     4441 tgctacaatt cgatggcgaa ggaggggcaa acatgtcctc taacgcgaag aatttacgct
     4501 atgtgattac gggattagat aatatttcat tccttgactg tagtgtcgac gtccggattt
     4561 ccccggaaag tcagatagtc aattttgggc agatcgctgc gaattccatt gcaactttcc
     4621 caccgaaggc agcattcagc gtttctacca taaaagacat tgcgtctgat tgtaccgaac
     4681 agtttgatgt tgcaaccagt ttctttactt cagatacatt atatgacaat acgcatctgg
     4741 aaataggtaa cggcttgctc atgcgaatta ctgatcaaaa aacgcaagaa gatattaaat
     4801 ttaaccagtt caaattattt agtacttata ttcccggtca gagtgcggca atggcaaccc
     4861 gcgattacca ggccgaatta acccaaaaac ctggtgaacc actcgtctat ggcccatttc
     4921 agaaagacct gatagttaaa atcaactacc actaaacagc aaaatgccgg gaacaccccg
     4981 gcattttcat atttagtatt tcatctcatt agctggcttt acgctcatgt gcctgacggt
     5041 atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca aactcaatgc
     5101 actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagc acaccagctg
     5161 gtttaaagcc tgccagcgtc atcagatcga tagtcgcttc agtatgaccg ccacgcgtca
     5221 gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc cggacgattc agatctgacg
     5281 gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acggtcagcg gcagaaacac
     5341 cggtagtcac accttcagct gcttcaatgg tcacggtaaa accggtgcca taggcgctgg
     5401 tgttattttc taccatcatt ggcagatcga gttgtttacg gcgatcgtca gtaatgcaca
     5461 ggcaaacaat accactcccg tggcgaatgg tcagcgccat ctgctcaacg gtcatggttt
     5521 ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc atcatcaagc accatcacac
     5581 cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc gtaccaaaag
     5641 aggaaagtag cgtctgattc atggtaaaaa aacctcacta aaattatggt taccagaatc
     5701 agggcagtct taggagtggc ggcatatagc caaaataacg tgagcgggtc catgcccgac
     5761 agaatcgtta ctctctccca tccggactct aaccgtcggc cccggaatta caccggatct
     5821 gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt caactgagtt gatttaccgc
     5881 cggtggggaa tttcgccccg ccctgagaat aagcggattc actataacgc taatgattag
     5941 cgccagcaac gcatagcttc acataattct ggtttatgac ttacccttat cgcactacaa
     6001 tggcactcaa cacctatcag tacagggaaa ctacaatgat tgacccgaaa aaaattgagc
     6061 aaatcgctcg ccaggttcac gaatcaatgc ctaaaggaat cagggagttc ggggaagatg
     6121 tggagaaaaa aatccgccaa accctacaag cgcagctgac gcgtctcgat ctggtaagcc
     6181 gcgaagaatt cgacgttcag acgcaagtcc tgttacggac tcgtgaaaaa ctggcgttac
     6241 tggagcagcg catcagcgaa ctggaagcgc gcaataatcc gatagcagaa cttcaatctc
     6301 ctccggctat cccgccagtt gataaagcag aataaagcac cacaaaaacg ggccagacgg
     6361 cccgtaatat cagtgttcta attcaagttc tctggtttgc atcatctgtt ggcaatacca
     6421 ggcataacgt gcgcaaaacc aggttctatg ttcttcatcc atgttaccag taacggccag
     6481 aatatcgacc gggcgacctt ctgcgtgcat tctggcaaaa ctacgcgcga gaaaatcgaa
     6541 attatttaaa gaattaagac tatgatccat aagcacatcc tccggttatt gcataccttt
     6601 taaggccata tgctttttct gatttcagta ttccccaatc agtgtagtgc ctttaaacat
     6661 aacgtgcgta aatatattgt ccccgatcac actttttagt gaaaaataaa aaaaccatat
     6721 taaaagactt tctcttccag aacctctcta acatgtgtta ttattaacaa agataaatgt
     6781 aagcaaggaa actattccaa agtaaatatt gcgtcactaa atggacattg gagtgacata
     6841 tgattttatt cgccgactat aacacccctt atcttttcgc tatttccttt gtactgttga
     6901 tcggcctttt agaaattttt gcacttatct gcggtcacat gctctccggt gctcttgatg
     6961 cacatcttga ccattatgat tccataacca caggtcatat tagccaggca cttcattatc
     7021 tcaatattgg aagattacca gctctcgtcg tcctctgctt actcgctggt ttttttggtc
     7081 ttattggcat cctgttgcaa catgcctgcg ttacgctctg gcaatcacca ctccccaacc
     7141 tgttcgtggt tcctgtcagt ttactgttta caattattgc agtgcattat accggtaaag
     7201 tcgttgcacc ctggattcca cgcgaccata gttcagctat tacagaagaa gaatatgttg
     7261 gtagcatggc attaattacc ggccatcaag ccacttcagg taacccctgc gaaggaaaac
     7321 tcaccgatca atttggccaa atacattatt tgctactgga gcctgaagaa ggaaaaactt
     7381 tcactaaagg agacaaagtg ttaattattt gccgactttc tgcaacgcga tatttggcgg
     7441 aaaataatcc ctggccccag atattgtgat ttattaatca aaaggaaatt taataatgga
     7501 tgatatcgtt aattctgtgc cctcctggat gtttaccgcg attatcgccg tatgcattct
     7561 gtttattatt ggaattattt tcgccaggct ctatcgtcgc gcttcggcag agcaagcatt
     7621 tgttcgtact ggtttaagtg ggcaaaaagt ggtgatgagc ggtggcgcaa tcgtgatgcc
     7681 gatctttcat gaaataattc ccatcaatat gaataccctg aagctggaag tcagccgttc
     7741 tactattgat agcctgatta cgaaagatcg tatgcgcgtc gatgtcgtgg ttgctttctt
     7801 tgtgcgggta aaaccttcag tagaagggat tgccaccgcc gcccagacgc tggggcaacg
     7861 caccctgtcg cctgaagact tacgtatgct ggtggaagat aaatttgtcg atgccctccg
     7921 tgcaacagcg gcacaaatga ccatgcatga gttacaggat acccgcgaga acttcgtgca
     7981 gggagtacaa aataccgtag ctgaagatct gtcgaaaaat ggcctggaac tggaaagcgt
     8041 ttcacttacc aactttaacc agacctcgaa agaacatttc aatccgaaca atgcctttga
     8101 cgccgaaggt ttaaccaaac tgactcagga gacggagcgc cgtcgtcgcg aacgtaacga
     8161 agttgaacag gatgtggaag tcgcagtgcg tgagaaaaac cgtgatgcac tttcgcgcaa
     8221 gttggagatt gaacaacaag aagcgtttat gacgcttgag caggagcagc aggttaaaac
     8281 ccgtaccgct gagcagaatg cgaaaattgc ggcttttgaa gctgaacgtc gtcgtgaagc
     8341 agagcagacg cgaattctgg ctgaacgaca gattcaggaa acagaaatcg accgcgaaca
     8401 ggccgtccgc tcaagaaagg ttgaggccga acgtgaagtt cgcattaaag agatcgaaca
     8461 gcagcaggtc accgaaatcg ctaaccagac gaaatcgatc gctattgccg ccaaatcgga
     8521 acaacagtcc caggcagaag cgcgtgctaa ccttgcactc gcagaagcag taagcgccca
     8581 acaaaacgta gaaaccactc gccagactgc cgaagccgat cgtgctaaac aagtggcgtt
     8641 gatcgccgca gcgcaggatg cagaaaccaa agcggttgaa ctgaccgtgc gggcgaaagc
     8701 agaaaaagaa gccgcagaga tgcaggcggc ggctatcgtt gagttagccg aagctacacg
     8761 taaaaagggt ctggcggaag cagaagcaca acgtgcgctg aacgatgcta tcaacgtact
     8821 ttctgatgaa caaaccagcc ttaaattcaa actggcatta ttgcaggcgc tgcctgcggt
     8881 aattgagaaa tccgtggaac caatgaagtc tatcgacggc atcaaaatta ttcaggtcga
     8941 tgggctgaat cgtggtggcg ctacgggtga tgcaaacacg ggtaatgtgg ggggcggaaa
     9001 cctggcagaa caagcgttat cagccgcact ctcttaccgc acacaggcac cgctgattga
     9061 ctccttgctc aatgaaattg gcgtttcagg cggctcactg gcggcattga cttcatcctt
     9121 aagctcaaca actcccgtcg aagaaaaagc agagtaagcc ctcttctatg aataatccct
     9181 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgatcagg
     9241 ctgttgcagc attttgcgtg actgctcggg gaaggtaaaa ctgcctctgg gaaagcgttg
     9301 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
     9361 cctcccgagg aaggggcctt gtataaggaa agggttatga tgaagctcgt catcatactg
     9421 attgtgttgt tactcgtaag tttcgcagct tattaacagc caatcagagg ggggagaaat
     9481 cctccctcac ccttgttcct ttactttagg ttgaaaaaaa atcagcgtca ataggcccgc
     9541 ctgctacaaa gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc
     9601 ttcttgatga tgttggtcgt cgagcaaccg tcttcaaagt tgagcaccag cacttcgcca
     9661 ccgttggccc agacttcttt actcccggca atctcttctg gtttatagtc gccgcctttc
     9721 accagtagat ctggcaagat cccggcgatc aagcgctgcg gcgtgtcctc ttcaaacgac
     9781 accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta
     9841 acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc
     9901 aaacggtcac ccagtttacg ggcatttgcc agataagaga cgtgcccagc gtgcaggatg
     9961 tcaaagacac cgttggtcat caccactttt tcaccgcgtt tacgcgctgc cgctacagca
    10021 agcttcagtt cctcttcggt catcacacca aagccggtat ctgcacgtcc acgtactgcg
    10081 ttttccagct cgatcggcga aaccgtggat gtccccagtt tgccgaccac gacaccagcc
    10141 gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc
    10201 aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt
    10261 ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc
    10321 actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct
    10381 tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc
    10441 gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacaccc
    10501 gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca
    10561 gaaagcacca gcgcgccaat cgaactcagt gcctgattaa tccgctcgtg cagcggctgc
    10621 ggatcaaccc cttcgaaacc ttcttcgaaa tccagacgga tcagttgttg gttgcgggaa
    10681 agtacccgta atttggtgat ggtcggatgc gtcggtacag aaacaaagtc gcatttgacg
    10741 ttgacgtcgg ccagagattt actgagcgcg cgcgccgcat cgtcaatgcc cgtcaacccg
    10801 accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc
    10861 ggacgttctt cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacgg
    10921 ctggtgggac cataccagta acgatccagc atcacatcac caaccaccat cactcctgca
    10981 cattcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc
    11041 gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagtt
    11101 cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat
    11161 gatgaagttc atcgcgcagc gttgtgtaag cacgggtcag cgccatcgct tcctgctctt
    11221 ccataatgtc gttttgcgcc agtagttcca gaatgcgcac gttgtctgac cagcgcgtta
    11281 acttcggttt ttcatgagcg tagcgcaaca ccagatattg agtaataaat tcgatatcgg
    11341 taattccgcc ttcatcggct ttgatatcaa agcgatcgcg atgtttattg ccgagatggg
    11401 cacgcatttt ctcgcgcatt tcccgcactt ctgtttgcag ggttttgcct tcgcgcggca
    11461 gcgtcataat ctcccggcgt actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca
    11521 ctacacgcgc acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat
    11581 cagcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg
    11641 catccacttc atacaaaatg ccggaagagg ttcgcgtact gaacagatgc ataatgcgtt
    11701 gcgccagacg cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat
    11761 ccattgggca atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc
    11821 cacccagctt gccgtagccg accaccgcaa aaccacgccc ttcgcgttcg ttcaggtgat
    11881 ttggcttacc gtagcgggca accatttgaa cccacgcctg ctgaacgacg gcatcaatca
    11941 tggcttctgc cagccaggtt aagtgatcgc tcactttcat taccggtagc gtaccggcga
    12001 tatccgctgc cgcaatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct
    12061 gttgttcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcagttca tcacggtagg
    12121 catccgtcgc cgttggttgg tagagggtat ttggatcgag caattcatcc agcaataatg
    12181 gataacgcgc tagctggctg gcaatcatcg gcgatgcagc acacagagaa atcaaatgtt
    12241 taagcgccgc agggaattca ctaagcagct caagataagt ggtgcgagta acaatcccca
    12301 ccagcaaggc ggtaatgcgc gacagcgtga cagcagcgtc ttcacgcgca cagacatcac
    12361 ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct
    12421 tatccagctc tttgcgaaaa tcagcaatca gcattagcac ctgtttgcga tcatcctcgt
    12481 taagatgcgc cagcactggc gtggtgtcat cttcctgcaa cgcatcctgc cacagctcac
    12541 gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat
    12601 taaacacccg gcgcacattg gccatatgtg cggtcagcac cccggtcaat tgcggccagt
    12661 cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgtct
    12721 gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatacg
    12781 ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca atggcgctga
    12841 gcgttggcag taaagcgcgc gattgcagcg acggttcgcg cccgccgcga atgagctgga
    12901 acacctggac gataaattca atttcgcgaa tgccgcctgc gccaagtttg atgttgtcgg
    12961 tcaaaccgcg tcgacgcact tcacgggcaa tcattccttt catgttgcgc agcgactgaa
    13021 tcacgctgaa atcgatgtaa cgacggaaaa caaacggacg cagcatcgca cgcaactcgt
    13081 tagcatagac gccgtcgcta tcccccataa tccgcgcctt gaccatcgcg taacgctccc
    13141 agtcgcgccc ctgctcttgg taataatctt ccagcgccgc aaaactcagc accagcgggc
    13201 cactttcacc aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg
    13261 gttgatccag cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca
    13321 gttcccggcg tccaccctgg gtacaaccat gttccggcca ggcaaaaatc agatcgatat
    13381 cagaggagaa attcagttcc ccgccgccca gcttacccat gcctaaaatc agcagcggtt
    13441 gcgcttcgcc ctgcgcattg cacggcgttc cccattcgcg gcagcaggcg tcatacagcc
    13501 agtcacgcgc cgcaacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct
    13561 cttcagtaac cagagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga
    13621 atagccgcag ctcgcgcatt aacccggctt cgtcactcac attactgagc gcctcctgca
    13681 accatgcagc gtaatgctgc cattcgtcgg cctgcggcgg ttggctttcc agttccgtca
    13741 gccactttgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact
    13801 tcgcctgtgc gctaagtgat tcctcggcta aaggctctgg cagccgctca acaacggtct
    13861 gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat
    13921 taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt
    13981 tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc
    14041 caggcttgcg cgacgacagg atcgtagtaa cccgccagca acagtattga gtcaatatca
    14101 cgcgtcaggc gtggcaattg gtcacggtag cgatcgccta acggctggca gaaaacgctt
    14161 ttcagttcag cggcatagcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctg
    14221 attttgctct gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg
    14281 gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga ccgccgttac ggcagaaacc
    14341 gcagaagcaa tggtcgcctc gcattgagtc agcagatcac gtaagtgagt gctcgcttta
    14401 cgcggcacga taccgccgaa cagcatcagt gtatgacgga ccaggccaat ggctgccagc
    14461 acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc
    14521 gctaacgcca gctcgagcgc cgcttccagc ccctgttcca catcagcttt tgccgcaaca
    14581 tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca
    14641 cgcgctgctt tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc
    14701 agtttcagca ccgcacgcat gtcgccgcta agcagttcca gttccagctc acagataggt
    14761 tcagcaaatt cacccacttt cacttccccc tggtcgaggg cgatttcaat ttggctacca
    14821 tcaacctcta ccagccactt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg
    14881 gaagcgagat cggcaggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg
    14941 tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc
    15001 actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgcaag
    15061 cccatatcgt gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc
    15121 acggggtcat ggtgctcgcc gtccagcgta ttgagatggt cacgcagcgc ctcaacggca
    15181 ctgtgattaa caataaactt taattcgatt tcctgagcca tccttcctcc actgggtact
    15241 ccccccaacg taggcgggag gggaatttgg gctgttaagc ccgcagttta tcagccacgc
    15301 tgattctgaa tatacttttt cacaacttcc agcggtgctc caccacatga accgatgaaa
    15361 taagagcgtg accagagtac gggctttgaa tacgcggcgc gcaaatcaat aaaatcattc
    15421 cgcagccgcc tactggtaac ggacttaagg ctgtttacca gctttgtgag ttgcaccaac
    15481 ggtgggtatt caatcagcat gtgaacatga tctgcctcgc cgtcacattc ctttaactcg
    15541 gcgccaaagt ccgcgcatac ctccgctaca tactgacgca tgtttgcatg atgcaattca
    15601 ccaaaaactg gacggcggta ttttgtcata aaaacaacgt gaacatgcaa caggtaagct
    15661 gaatagcgtg aacgattgat ttcgcacacg tattgaccct cccaaaaaaa tgtggatata
    15721 ttgtacattc taaacaacca catactgaat gcaaaatgtt aatccagaaa gcatataaat
    15781 acaggctcta ccccactgac cagcaggcgc aaaggttgcg tcagttgtgc ggttgcgctc
    15841 gctttgtctg gaactatgca ctgaatgaaa cactgagcat tcatgacgcg ggcggaaaaa
    15901 ttccgtcagc tttcgacctg aataaacgac ttacgggctg gaaaaaactc cctgagctgg
    15961 cctttctttc tgagggttac actgacaacc tccagcagaa actgaaagac ctgcggagcg
    16021 catgggatcg ctgttttgat aaatccctga cggctgaaaa gcccgttttc aaaaagaaaa
    16081 ccaaaggctg cgactctatc cggttcgtca atttcagcaa atattgtggg ctggattatg
    16141 gccgtgtgaa actcccgtca ggactgggct gggttaaatt tcgtcagtcc cggaagattg
    16201 aaggcgtcat taaaaattgc accatcagcc agcatgccgg acactggtat gtgtcttttc
    16261 aggtcgaatt agctgttacc gatccgattc acgcatcgac cagcgcaata ggcctggatg
    16321 caggtataac gaagctggca acactttcag acggcacggt ctttgaaccg gtaaacagct
    16381 ttaaaaaaaa tcaggataag ctggctcgct tacagcgccg tctggcccgg atggttaaat
    16441 tctccgccaa ctggaaaaag cagaaagcga agatttcccg tttccactct catatcgcaa
    16501 acatccgccg cgattacctt cataaaacca cgacgacgat cagcaaaaac cacgcgatga
    16561 tagtcattga ggatttaaag gtttcgaaca tgtcaaaatc agcagcgggt acggtagacc
    16621 agccggggcg caacgtcgcg gcaaaatcag gtcttaaccg ggcgatactg gatcagggct
    16681 gggcagaaat gcgccgccag ctcgaatata agcaggcatg gcgcggcggt gatgtactgg
    16741 cgattaatcc ggcgtacacc agtcagaaat gcgcatgttg tgggcataca tcgaaaaata
    16801 accgcaggac gcaggcgagc tttatctgta ccgcgtgcgg ctatacggca aacgccgatg
    16861 taaacggcgc tcgcaatata ttaacagcag gttttgaagt catggcggca ggacaaatat
    16921 tgccgtcagt ccgtaaagga cgcgcccgta aggcggcgta agccgtcgag cgaggaaccg
    16981 gggaatgcgg caacgtattg ctaccctgac aagggaatcc ccctcctgag tcgctttgcg
    17041 cgaaggtggg ggaggatgtc aaggccttta cttatcggtt atgtcacata agtgacgatg
    17101 aacggcgaac ttaatgcgat cttttttgtc agtagatagt attttgcgcc aaattgccat
    17161 gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg gcaagacctg acacagagga
    17221 ttccgattga tgtcgaatgc tttgcgtagc gacactgatg ccactactat cgttccactt
    17281 tccatgacaa taacgacagc ctgatgccaa aattacgcct gatcggatta actttactcg
    17341 cacttagcgc cactgccgtc tcacacgctg aagaaacgcg ctatgtttct gatgaactga
    17401 atacctgggt ccgtagcggt ccgggagatc attatcgcct cgtgggcacg gttaacgccg
    17461 gcgaggaagt gaccttatta caaactgacg ccaacactaa ttatgcccag gtgaaagaca
    17521 gctctggccg tactgcctgg atcccgttga aacaacttag cacggagcca agcctgcgtt
    17581 cccgtgtgcc agatctggaa aatcaggtca aaacgctgac cgataaactc accaatatcg
    17641 ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa agtggcgcag agtgacagcg
    17701 tgatcaacgg gttaaaagaa gaaaatcaga aactgaaaaa cgagctgatt gtcgcgcaga
    17761 aaaaggtcga tgccgccagc gtacagctgg atgacaaaca gcgcaccatc atcatgcaat
    17821 ggtttatgta tggcggcggc gtgctggggc ttggcttgct gctcggtctg gtactgccgc
    17881 acctgatccc aagccgcaaa cgcaaagatc gctggatgaa ctaaatcgcc ttcttcgttg
    17941 cactgtctta ttattaggaa ttgtgtattt gacgaaggga tgatggcgtg aagatttatc
    18001 tggtcggtgg tgctgttcgg gatgcattgt tagggctacc ggtcaaagac agagattggg
    18061 tggtggtcgg cagtacgcca caggagatgc tcgacgcggg ctaccagcag gtaggccgcg
    18121 attttcctgt ttttctgcat ccgcaaacgc atgaagagta tgcgctggca cgtactgaac
    18181 ggaaatccgg ttccggttac accggtttta cctgctatgc cgcaccggat gtcacgctgg
    18241 aagatgatct taagcgtcgc gatctgacca ttaatgcgct ggcccagaac gataacggcg
    18301 agattatcga cccgtacaac ggtctgggcg atctgcaaaa tcgtctgttg cgccatgttt
    18361 cccccgcttt tggcgaagat ccgttacgcg tgttgcgcgt agcgcgtttt gctgcgcgtt
    18421 atgcccacct cagtttccgt attgccgatg aaactctggc gctgatgcgc gagatgaccc
    18481 atgcgggtga actggaacac ctgacgcctg aacgggtatg gaaagagacg gaaaacgccc
    18541 tcaccacccg caatccccag gtgttcttcc aggtactgcg cgattgcggc gcactgcgcg
    18601 ttttattccc ggaaattgac gccctgtttg gcgttccggc ccctgccagg tggcatccgg
    18661 aaatcgacac gggtattcat accttaatga cgctctcaat ggcggcgatg ctgagtccgc
    18721 aggtcgatgt ccgtttcgca acgttatgcc acgatctcgg taaaggactg acgccaccag
    18781 aactttggcc gcgtcatcat ggtcatggcc cggcgggtgt gaagttagtg gaacaactat
    18841 gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc cagactggtg gctgagtttc
    18901 acgatctcat ccacaccttc ccaatgctga acccaaaaac catcgtcaaa ctatttgatt
    18961 ccatcgacgc ctggcgtaaa ccgcagcgtg tcgagcaact ggcgctgacc agcgaggctg
    19021 atgtgcgcgg cagaaccggt tttgaatcag ccgactaccc gcaaggccgc tggttgcgcg
    19081 aagcctggga agtggcacag tcagtgccga caaaagccgt cgttgaagcg ggatttaaag
    19141 gtgtggagat tcgcgaggag ctgacccgac ggcggattgc ggcggtggct ggctggaagg
    19201 aacagcgttg cccaaaacct gagtgagtgt tggatatttt gccggatgcg acgtaagcgt
    19261 cttatccggc tacatgtcaa cgacagttgt aggcctgata agacgcgcga gcgtcgcatc
    19321 aggcgttgat tgccggatgc ggcgtgaacg ccttatccgg cctacgcaat ctgataagac
    19381 gcgtcagcat catatccgac actgttcttc acatcaaaag aacacaacgt acaccgcagc
    19441 cgccacaata aagcgataaa tggcgaacgg gataaacgaa atgcgcttaa tcaattgcag
    19501 gaaggtttta atcgctatca gcgccaccac aaaagcggtg ataaacccaa cggcaaacat
    19561 cgggatatcg ccggttgtca ggaagcccca gcttttgtag agatcgagcg ccgttgcgcc
    19621 catcatcatc ggcaccgcca gcaggaacga aaactcggaa gcagcgtaac ggctcacccc
    19681 catcagcatc ccacctgaaa tggtcgcccc ggaacgggag aaacccggcc acagcgccag
    19741 acactggaaa cagccaatca taaacgcctg acgataggtc atatcatcaa gacccggcgc
    19801 acgcggctct ttcggcttca ggcattcggc ggcaatcagc aacagaccgc caacgaccag
    19861 cgcatacatc acatttatcg ggttaaacaa tgacttaatc gtgtcgtgga acaacagccc
    19921 cagtaccacc gccggaatca tccccagcaa aatgtggatc agcgttaaac ggcctttgct
    19981 ttctccttcg tgctgcaacg ggcgaccaaa gtgaatacca ataaggccaa acagacgtcg
    20041 ccagaacatg acaaccactg ccagaattga tcctaactgg atcacgactt caaaggtttt
    20101 cgccgtgtag ccctcaaacc ccaacaagtg accgacaata atcatatggc ccgtgctgga
    20161 taccggcaga aattctgtca atccttcgac cacacccaat attgccgcta tcagcagcga
    20221 gtgcatatcg ctcatcaata aacccctaaa ttataaaaat gtaacgcttg tccgaactac
    20281 tgcagtatga ccaggttata accgtttggt ttaacagctg taaaattaat tattttcttt
    20341 cagattattg ccacgctcaa tgatgacgcc aacattcgcc gcccgcgcca ctgcgcctgg
    20401 cttgctgagt ttgatacgca cccacggcga gttgaagcgt gctaacagca gctccgccac
    20461 ctcttcagcc acgcgttcca ccagcgcaaa acgcgccccc tcgacgtggc tgaccaccgt
    20521 ttctgcaatg tcagcgtaac tgaggcaatc cgccacatca tcacttttcg ccgctttacg
    20581 gttatcccac gccatttcga tatcgaacac taacttctgt tcgatggtct gttcccagtc
    20641 gtaaacacca atggtggtga ttaccgaaag ttgctctata aatacaatat ccatcacgtc
    20701 ctgcctgctt tttggctaac cggataccac ttccggcgaa atgtgcgtat tatccacaga
    20761 ttcatcgttg aacacgaatt ttcaaaacgg aacagcttat gagtgcaatc gcgcctggaa
    20821 tgatcctcat cgcgtacctc tgcggctcca tttccagtgc cattctggtt tgccgcttgt
    20881 gtgggttgcc cgatccgcga accagcggct ccggcaatcc aggcgcaacc aatgtgttac
    20941 gtatcggtgg caagggagca gccgtagcag tactgatttt cgacgttctg aaaggaatgt
    21001 tgcccgtctg gggcgcgtat gaattaggtg tcagcccctt ctggctaggc ttaattgcca
    21061 tcgccgcctg tcttggacac atctggcccg ttttcttcgg ttttaaagga ggaaaaggcg
    21121 ttgctaccgc ttttggtgcc atcgcaccaa ttggctggga tctcactgga gtaatggcgg
    21181 gaacctggtt actgaccgtg ctattgagtg gttactcgtc gctgggagcg attgtcagtg
    21241 cactgattgc tccgttttat gtctggtggt ttaagccaca attcaccttc ccggtttcga
    21301 tgctctcttg cctgatcctg ctgcgtcatc atgacaacat ccagcgtctg tggcgtcgtc
    21361 aggagacaaa aatcaggacg aaattcaaaa gaaagcgcga aaaggatccc gagtgatttc
    21421 tggtggatct acatgacctg atagccttcg tcgggcttgc ccagccgttg ctggcaccat
    21481 gccgccagaa attccacaca gacacgtaat ttcatgctgc gatagagcgg ctcccggtaa
    21541 acagcccaga tattggcgct ttgtgcatac tccggcaata ctcgcaccag tttgccactc
    21601 tccagaaacg gcaacacatc ccactcggaa cgcaacataa tccctttgcc ctccagcgcc
    21661 cattgcaaca ctatctcgcc gctattggag gaaagatgcc cgcttacctt caccgatttt
    21721 ttctcctgac cgttccccaa ctcccatatt ccgtgggtca tatcgcgctc tttggtcacc
    21781 agacaatcat gacgacttaa ttcctgtaag gattgtggct gcggatattt ttgcagatac
    21841 tcaggtgctg cgcataatat tcttttattt ttcgttaaca gatgcgcaat ataataatca
    21901 ggaatttcgt cattaatacg aatatccaga tcaatattat cctgcactaa atcaatttgc
    21961 cgatcgaaca attcaaaatg cacctgtaac tcaggataat tgcgcatcag ttcggtaatg
    22021 gctggcgcaa tatggctgcg cccaaaacca aaactgcaac caatacgaat catcccctcc
    22081 gggcgcgttt tgatttgcgt gacgtcatcg accagtcgct gatattgggt gaggatttcc
    22141 agcgcatgct cgtaacaacg ttggccactt tcagtcaacg ccacaccgcg agcggagcgg
    22201 ttcagcagcg ttgttgccag cgtattttca aggatttgga tccgcttggt gacaaatgca
    22261 ggcgtctgcc caagcgtcgc cgctgcagca ctaaaactcc cgctgtggac gatttctacc
    22321 agtacctgca aatctttggc taaaggccag ctattcagca tcgttaatta tccgcagttg
    22381 tgataagcgc agtgtatttc gcaaaacatt gccattccct gctctggcgc aatattcatt
    22441 cttattttcg cgttgattca cgaactgtga attacatatt cacggcggca aaaaatataa
    22501 aaccacattt ttagtggtag tttgtggcgg tgaatttttc cagacaaata cataaactgg
    22561 agttgccatg atgagcgaaa gtaataagca acaggcagtg aataagttga cggagattgt
    22621 cgctaacttt accgccatga tttctacccg aatgcccgat gacgtggtgg ataaactaaa
    22681 acagctaaag gatgccgaaa catcgtcgat ggggaaaatt atctaccata cgatgttcga
    22741 caacatgcaa aaagcaatcg acctgaatcg cccggcctgc caggacaccg gggagattat
    22801 gttcttcgtt aaagtcggtt cccgtttccc gctgcttggc gagctgcaaa gcattcttaa
    22861 gcaagctgtg gaagaagcaa ccgtcaaagc gccactgcgc cacaacgcgg tggaaatttt
    22921 tgacgaagtg aacaccggca aaaataccgg cagcggcgta ccgtgggtca cctgggatat
    22981 cgtccccgac ggtgacgatg cggaaatcga agtttacatg gcaggcggcg gctgcacgtt
    23041 gcctggtcgt tcgaaagtgt taatgccgtc ggaaggctac gaaggcgtgg tgaaattcgt
    23101 cttcgaaaat atctccaccc tcgccgtaaa cgcctgtcca ccggtactgg taggcgtggg
    23161 catcgccacc tcggtggaaa ccgccgctgt gctctcacgt aaagccattt tgcgcccaat
    23221 tggcagccgc caccccaatc cgaaagcggc agagctggag ctacgcctgg aagaagggct
    23281 caaccgtctg gggattggtc cacaagggct gactggcaac agttcagtga tgggcgtaca
    23341 tatcgaatct gccgctcgcc atccgtcaac catcggcgtt gctgtttcta caggctgctg
    23401 ggcgcatcgt cgcggcacac tgctggttca tgccgatctc acctttgaaa atctgtctca
    23461 cacccggagc gcgttatgaa aaagatcctg acaaccccga tcaaagctga agatctgcaa
    23521 gatattcgcg tcggcgatgt gatctacctg accggtacgc tggtgacctg ccgtgacgtt
    23581 tgtcaccgtc gcttgatcga actgaaacgt ccgatccctt acgatctcaa cggcaaggcg
    23641 attttccacg ctggccccat cgtgcgcaaa aacggcgaca aatgggagat ggtatccgtc
    23701 ggcccgacaa ccagtatgcg tatggaaagt tttgaacgtg aatttatcga acagaccggc
    23761 gtgaaactgg tggtcggcaa aggcggaatg gggccactga ccgaagaagg ctgccagaaa
    23821 ttcaaggcgc tacatgtaat tttcccggca ggctgcgcgg tgctggcggc aacccaggta
    23881 gaagagattg aagaggtgca ctggacagaa ctcgggatgc cggagtcgct gtgggtctgc
    23941 cgggtcaaag agttcggtcc gctgattgtc tctattgata cccacggcaa caacctgata
    24001 gccgaaaaca aaaagctgtt cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag
    24061 cacgtccatt acattaaata accctcccgg agaggctcgc cccctctcct ttttcgcagg
    24121 cataacacga tgaaaccttc cactgaatgg tggcgatacc ttgcgccgct ggcggtcatc
    24181 gccattattg ctctaattcc ggttcccgca gggctggaga gtcatacctg gctctacttt
    24241 gccgttttta ctggcgtgat cgttggactg atcctcgaac ccgtgccggg tgccgtggtg
    24301 gcgatggtgg gtatctccat tatcgccatt ctctcgccgt ggctgctgtt tagcccggag
    24361 cagctcgccc aaccaggttt taagttcacc gccaaatccc tctcgtgggc ggtttctggt
    24421 ttttctaatt cggttatctg gctgattttc gccgccttta tgtttggcac aggctatgaa
    24481 aaaaccgggc tggggcgacg tatcgcgctg attctggtga aaaagatggg gcatcgtacg
    24541 ctgtttctcg gctatgcggt gatgttctcc gagctgatcc tggcacctgt aacaccgtcc
    24601 aactcggcgc gcggtgcggg gattatctat cccatcatcc gtaacctgcc accgctctat
    24661 caatcacaac caaacgacag cagttcgcgc agcattggct cgtacatcat gtggatgggg
    24721 attgttgccg actgcgtgac cagcgccatt ttcctgacgg cgatggcacc taacttgctg
    24781 ttaattggcc tgatgaaaag cgcatctcac gccacactga gttggggcga ctggttcctc
    24841 gggatgttgc cgctcagtat tttactggtt ctgctggttc cctggctggc ttacgtgctg
    24901 tacccgccgg tactgaagtc tggtgatcag gtgccgcgct gggcagagac ggaactgcag
    24961 gcaatgggcc cgctctgttc tcgtgaaaaa cggatgctgg ggctgatggt aggcgcactg
    25021 gtgctgtgga tttttggcgg tgattatatc gatgccgcga tggtcggtta cagcgtggtg
    25081 gcgctgatgc tgcttctgcg cattatcagt tgggacgaca ttgtcagtaa tagagcggcg
    25141 tggaacgttt tcttctggct ggcctcgctt atcaccctcg ctaccggact caacaacacc
    25201 ggttttatta gctggtttgg caaactgtta gcaggcagct taagcggtta ttcgccaacg
    25261 atagtgatgg tggcgttgat tgtggtcttt tatttgctgc gctacttttt tgccagcgcc
    25321 acagcgtata cctccgctct cgcaccgatg atgattgccg ccgcgctggc gatgccggaa
    25381 atcccgctgc cggtgttctg cctgatggtt ggtgcggcaa ttggtctggg gagcattctt
    25441 acaccatacg ccaccggacc cagtccgatt tactacggca gtggttatct gccaacggcg
    25501 gattactggc gactgggggc gatttttggg ctgatattcc tcgtattgct ggtgattacc
    25561 ggcttactgt ggatgcccgt ggtgttgctt taagtgagga cggtgccgga agttgctctc
    25621 cggcacctaa tagttttacg cagccggtaa ctccgccagc ggccagcgcg gacgcacact
    25681 cacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc attccggcat aggcgatcat
    25741 cgcaccgtta tcagtgcaaa attccggacg cgcgtagaac acttcgccac gacgcttttt
    25801 catcatttcc gccagtttcg cccgcagtgt gcggttagca ctcacgccgc ccgccattac
    25861 cagacgcttg aagcccgtct gatccagtgc tcgcttacac ttaatcatca acgtatcgac
    25921 cactgcatct tcaaaggcgc gggcgatatc ggcacgcgtc tgatcgtcag tgccgttgtc
    25981 acgtatggta tttgccgcga aggttttcag accagaaaag ctgaaatcca gccccggacg
    26041 gtcggtcatc gggcgcggga agacaaagcg cccggcagta ccctgagccg ccattttcga
    26101 cagtaatggc ccgccaggat aatccagccc cagcagcttc gcggttttat caaacgcttc
    26161 accagccgca tcatcgatag actctccgag cagctcgtac tgaccaatgc cagtcacgct
    26221 gattaactgc gtatgaccgc cggaaaccag cagcgcgaca aacggaaatt ccggtgggtt
    26281 atcttccagc atcggcgcta acagatgtcc ttccatatga tgtaccggga ttgccggaac
    26341 gtcccaggca aacgccagag aacgccccac ggtcgcgcca accagcagtg cgccgactaa
    26401 tcctgggcct gcggtatagg ccacagcatc aatgtctttt gccgttaaac cagactcctt
    26461 tagcgcctcc tggatcaacg gtacggtttt acgcacgtga tcgcgggagg ccagttcagg
    26521 cacgacgccg ccgtagtcag cgtgcaattt cacctgacta tacaattggt tggctaacaa
    26581 acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg caggaagttt caatacccag
    26641 tacacgcatg acttgtttta cctcgcttta ttaccgcgca gtgtaggacc aatgcgggtt
    26701 gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta tactcctcac ccttataaaa
    26761 gtccctttca aaaaaggccg cggtgcttta caaagcagca gcaattgcag taaaattccg
    26821 caccattttg aaataagctg gcgttgatgc cagcggcaaa ccgaattaat caaaggtgag
    26881 aggcacatgc cggtaattaa agtacgtgaa aacgagccgt tcgacgtagc tctgcgtcgc
    26941 ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag ttcgtcgtcg tgagttctat
    27001 gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg cagtgaaacg tcacgcgaag
    27061 aaactggctc gcgaaaacgc acgccgcact cgtctgtact aattccccga gagcgttgct
    27121 ctccgatcag accgagttgt agttgtaagg ccgtgcttcc gaaaggaatg cgcggcttat
    27181 tttcgtttat gaattgctaa aaatcggggc ctatggctgg acgaatccca cgcgtattca
    27241 ttaatgatct gctggcacgc actgacatcg tcgatctgat cgatgcccgt gtgaagctga
    27301 aaaagcaggg caagaatttc cacgcgtgtt gtccattcca caacgagaaa accccgtcat
    27361 tcaccgttaa cggtgagaaa cagttttacc actgctttgg atgtggcgcg cacggcaacg
    27421 cgatcgactt cctgatgaac tacgacaagc tcgagttcgt agaaacggtc gaagagctgg
    27481 cagcaatgca caatcttgaa gtgccatttg aagcaggcag cggccccagc cagatcgagc
    27541 gccatcagag gcaaacgctt tatcagttga tggacggtct gaatacgttt taccaacaat
    27601 ctttacaaca acctgttgcc atgtctgcgc gccagtatct ggaaaaacgc ggattaagcc
    27661 acgaggttat cgctcgcttt gcgattggtt ttgcgccccc cggctgggac aacgtcctga
    27721 agcggtttgg cggcaatcca gaaaatcgcc agtcattgat tgatgcgggc atgttggtca
    27781 ctaacgatca ggggcgcagt tacgatcgtt tccgcgagcg ggtgatgttc cccattcgcg
    27841 ataaacgcgg tcgggtgatt ggttttggcg ggcgcgtgct gggcaacgac acgcccaaat
    27901 acctgaactc gccggaaacg gacattttcc ataaaggccg ccagctttac ggtctttatg
    27961 aagcgcagca ggataacgct gaacccaatc gtctgcttgt ggtcgaaggt tatatggacg
    28021 tggtggcgct ggcgcaatat ggcattaatt acgccgttgc gtcgttaggt acgtcaacca
    28081 ccgccgatca catacaactg ttgttccgcg cgaccaacaa tgtcatttgc tgttatgacg
    28141 gcgaccgtgc cggccgcgat gccgcctggc gagcgctgga aacggcgctg ccttacatga
    28201 cagacggtcg tcagctacgc tttatgtttt tgcctgatgg cgaagaccct gacacgctgg
    28261 tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga gcaggcgatg ccactctccg
    28321 catttctgtt taacagtctg atgccgcaag ttgatctgag tacccctgac gggcgcgcac
    28381 gtttgagtac gctggcactg ccactgatat cgcaagtgcc gggcgaaacg ctgcgaatat
    28441 atctacgtca ggaattaggc aacaaattag gcatacttga tgacagccag cttgaacgat
    28501 taatgccaaa agcggcagag agcggcgttt ctcgccctgt tccgcagcta aaacgcacga
    28561 ccatgcgtat acttataggg ttgctggtgc aaaatccaga attagcgacg ttggtcccgc
    28621 cgcttgagaa tctggatgaa aataagctcc ctggacttgg cttattcaga gaactggtca
    28681 acacttgtct ctcccagcca ggtctgacca ccgggcaact tttagagcac tatcgtggta
    28741 caaataatgc tgccaccctt gaaaaactgt cgatgtggga cgatatagca gataagaata
    28801 ttgctgagca aaccttcacc gactcactca accatatgtt tgattcgctg cttgaactgc
    28861 gccaggaaga gttaatcgct cgtgagcgca cgcatggttt aagcaacgaa gaacgcctgg
    28921 agctctggac attaaaccag gagctggcga aaaagtgatt taacggctta agtgccgaag
    28981 agcgatcggg aagcccccga cagccgcact gagaggcagc ggcaaatata taagtacgcc
    29041 ctcgtaatta tcgttggcgg taaacaaccg ttggatttca gcgttaacgg ctgaaggaca
    29101 tcggatcaat cgcccaacac caacctcatg aaataagtgt ggataccgtc ttatggagca
    29161 aaacccgcag tcacagctga aacttcttgt cacccgtggt aaggagcaag gctatctgac
    29221 ctatgccgag gtcaatgacc atctgccgga agatatcgtc gattcagatc agatcgaaga
    29281 catcatccaa atgatcaacg acatgggcat tcaggtgatg gaagaagcac cggatgccga
    29341 tgatctgatg ctggctgaaa acaccgcgga cgaagatgct gccgaagccg ccgcgcaggt
    29401 gctttccagc gtggaatctg aaatcgggcg cacgactgac ccggtacgca tgtacatgcg
    29461 tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa attgacatcg ctaagcgtat
    29521 tgaagacggg atcaaccagg ttcaatgctc cgttgctgaa tatccggaag cgatcaccta
    29581 tctgctggaa cagtacgatc gtgttgaagc agaagaagcg cgtctgtccg atctgatcac
    29641 cggctttgtt gacccgaacg cagaagaaga tctggcacct accgccactc acgtcggttc
    29701 tgagctttcc caggaagatc tggacgatga cgaagatgaa gacgaagaag atggtgacga
    29761 cgataccgcc gatgatgaca acagcatcga cccggaactg gctcgcgaaa aatttgcgga
    29821 actgcgcgct cagtacattg taacgcgtga caccatcaaa gcgaaaggcc gcagtcacgc
    29881 tgccgctcag gaagagatcc tgaaactgtc tgaagtattc aaacagttcc gcctggtgcc
    29941 gaagcagttt gactacctgg tcaacagcat gcgcgtcatg atggaccgcg ttcgtacgca
    30001 agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa atgccgaaga aaaacttcat
    30061 taccctgttt accggcaacg aaaccagcga tacctggttc aacgcggcaa ttgcgatgaa
    30121 caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa gtgcatcgcg ccctgcagaa
    30181 actgcagcag attgaagaag aaaccggcct gaccatcgag caggttaaag atatcaaccg
    30241 tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag aaagagatgg ttgaagcgaa
    30301 cttgcgtctg gttatttcta tcgctaagaa atacaccaac cgtggcttgc agttccttga
    30361 cctgattcag gaaggtaaca tcggtctgat gaaagcggtt gataaattcg aataccgccg
    30421 tggttacaag ttctccacct acgcaacctg gtggatccgt caggcgatca cccgttctat
    30481 cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg attgagacta tcaacaagct
    30541 caaccgtatt tctcgccaga tgctgcaaga gatgggccgt gaaccaacgc cggaagaact
    30601 ggctgaacgc atgctgatgc cggaagacaa gatccgcaaa gtgctgaaga tcgccaaaga
    30661 gccaatctcc atggaaacgc cgatcggtga tgatgaagat tcgcatctgg gggatttcat
    30721 cgaggatacc accctcgagc tgccgctgga ttctgcgacc accgaaagcc tgcgtgcggc
    30781 aacgcacgac gtgctggctg gcctgaccgc gcgtgaagcg aaagttctgc gtatgcgttt
    30841 cggtatcgat atgaacaccg accacacgct ggaagaagtg ggtaaacagt tcgacgttac
    30901 ccgcgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc aaactgcgtc acccgagccg
    30961 ttctgaagtg ctgcgtagct tcctggacga ttaatcggta ggccggatca ggtgttacgc
    31021 cgcacccggc actagtccct ccgcacaaac gccacctttt cggtggcgtt tttatcgccc
    31081 acgcaccacc agcgcctggt ccagctcgcg atacgcttca accagtttct ccagtgaaac
    31141 gcgacttaaa ccgctgggat ttggcaggac ccaaatctgc gtcgaaccaa tggtgagagt
    31201 ttgtttcccc cactgtgcac cgcgctggct gaatccctgt tcatatgctt gtttgcccag
    31261 aatcgccaac gcctgcggct gataatcttc tattttttca attagcttac gcccgcctgc
    31321 gtgcagctcc tgctttgaca cttcattggc ttgcaccgtt ggacggtcta ccagtttggt
    31381 gacgccacaa cgataatcca gcagatgctg tgcctcctgc ggcttcaact gacggtcggt
    31441 aaacccggcc tgatatatca ccttccagaa gcggtttgcc ggatgagcaa agggaaaacc
    31501 cgttccggcg gatgaaagac cagggttgat accgcaaaac acgacccgta accctggagc
    31561 caaaatatcc tcaaccatat ttactccttt ctaacatcgc caggaaaagt ataacggatt
    31621 gaaaatacat tgtttataaa aacagcaggc gcgctgtaat ggctggattg cgacacggag
    31681 ttactttata atccgctacc atggcccctt agctcagtgg ttagagcagg cgactcataa
    31741 tcgcttggtc gctggttcaa gtccagcagg ggccaccaga tttcaatgaa ttacagatgt
    31801 atccaaacta tcttgcttta ccaggatact tataggatac ctctgagaag tattaacaag
    31861 taagaattgg ctcatgccta cagaacagtt gggtcgtagc agccgttcac ctgcgaacaa
    31921 gaaagtgcta cgggtctgaa cttttgcccc caactctcca agagaccggc aaaaaccctg
    31981 acggaggtat caatggatac tttaagaaag ggctcagacc tgctgtctgc acgtgtgatt
    32041 gccgttacag gtttaatcgc agcacttggc aatttaggtc tatgcccaat agccgtagtg
    32101 ctagtatgta tagctttagt tatcatgctg aagtaatgac agcgtaacaa gctcggccca
    32161 gctttcgctg ggcctcccat ttctacatcc caaaatctat tagaaacaat aagccactaa
    32221 agaaagagct tttgctggct acaatactca catacaaaca atttttattt ttcgcgggcg
    32281 taaaaaaacg atggggctgg cagtccggaa gacgtcaggg tacagagatt tgtacccgcc
    32341 tctccctgtg catgagcaca ataagtaacc gaaggcggaa ttccgccctc ggtactcaca
    32401 tatgagaaac gatatttctt ccaccgatac cctttatttg ggtatcgcga gtaagcccca
    32461 aaatctgggt gttactctta ctacccaaac tacgggtagt ttccgtagtt tcatggtcga
    32521 gttgcagatc tgcaactcac ccaccagcca acgcaatttt gcgttatcgg gaatatcagc
    32581 aagttaccac cgcagtcgtt ccggcttctt ccactggtaa gtatttttcg cgctctccct
    32641 ccgttgttga gaacggcgac ggtatgccag caactcaagg actctggttc gtatgttgcg
    32701 catatccacg ccgttaagct caataccgtc acggcgcatc acctcagcca ccacacgcgc
    32761 gtaattttca gcggtgacgc tgtccggctg cgtggcctcc tgtttgcctg ttgcctggct
    32821 gattccggta atactgcgga ttatttcgag cgcttcggtc tcggtcatgg tcatggcctc
    32881 catagtgatg aggttgatac cgtaattgta gcgggtcctc ctggtggggt ggcctgccac
    32941 ggggcgaaag catcgcggaa aaaggctagt ttttgaaatt tcattcgcca tcaccactgg
    33001 cacaactaat tgataatttg tgatttttat ttttacggtg tcggttttga ttgtttttcg
    33061 ctcaccacta acacatagcg cctaccagca tttatgaata atcccctggt gaatctcctg
    33121 cactacctgt attacgcaaa aaccctagca tttctaagca tgtatagagc cagttttgct
    33181 caaaaacgag aaaggaaacg acagaggcca aaaagcccgt tttcagcacc tgtcgtttcc
    33241 tttcttttca gggggtgttt caaataaaaa cataaagtta cggcgaagaa gaacggaaac
    33301 gccttaaacc ggaaaatttt cataaaaagc gaaaacccgc gcgcctgacg ccccgtagcc
    33361 taccggatcg ccggaaagga cccgccagcc agaacgggcc ctaatttcat caaccaatca
    33421 gcttatagcg accatcccgt gcattgcggc gtacacgatc aatcttgagg catagcgccg
    33481 catctggctt ttttgggaca ggtacgcggc aatattcaga agcgcgcgga atattgttta
    33541 tccagtcgat cacttcgctt aaataccagg ccttacgccc ttccgtaacc tgcacgcgtt
    33601 cggggaactc tccacgtgct tcaaggttta gcagtgtacg acgactcagg gttgtaagtt
    33661 ccatcacctg attcatatca acaagacgct cgcttaaacg cattttgtca gcaatagcct
    33721 ttaattcctc tactgctgga tccggataca tcatttcggc aattggctta aggtcattgt
    33781 aatgattctg cattgtatcc ccctttacac acgagccagc ggctgaacag aaatacctga
    33841 gccaacaaac gctgcaacct ttactgacag ttcttttact gactcaggcc agttaagagc
    33901 atcaacattt aagacacctg tcttatagac ctgagcctgt gtttttttcg ctgtatcgag
    33961 ttgtacagcg gaaacataaa cagctttacc tgcactcgta ccatcccata ccaccagtgc
    34021 acctgttgta tcttcctgca tcagtggcgt aaatgcagga attacccctt tattagctga
    34081 aaatatcccc agcgtagtca ccagtgcttc agtgccagcc atgagttcag tgtaatgaat
    34141 agccattgct ccccccttag ccaatgcgaa cggtaacaaa acgattgata cgggccggta
    34201 ttggctgtgg tgctgaatgt gtctgcacat attcaatagc cggatcacca ggcacaatat
    34261 agtttttcgg tgcaagttcg gctttagtca gccccatccg gattagctcc ggatcctgaa
    34321 taccgccata agcgacaatc ccctggagag ccgtattgcc aagcaccatc aaatcaggat
    34381 caaggaaata tttttcagtt ccgtcctcgt cggtataacg cccgctgtaa acaacaatcg
    34441 caacatcgcc catataccct ttaaaactca ccgaatcacc aaggtcttta agggccgttt
    34501 ccagttcgga attagaacca cgacgggtat ccagagcctc ttttatcgct ctgaatgaac
    34561 ggtatttctt ccatacatta ccgcccataa tgatgatatt agtgacgccc tcactaaatt
    34621 ctgcgtagct ctcaatatca tcatttggat caaaagtttc tttatcctta cctgaccact
    34681 cagtaccgcc agactgagtg atgatatttt gtggttttat attccagtcc agctcataac
    34741 gttcaatacc atcgccctca atgatatttt tccccgttgt gattgcctga acagcaagcc
    34801 attcaatacg tgtacgaata gctttagcct gatttacaat cgcctgttta actttaatat
    34861 tacgcgcccc aaaagcattg tattgctcag gtgacacacc agcagggcgc acagctaact
    34921 tatttggatc aatgctgctt ttcggtttca tatagccagg gcgaattgtt tttgattcgt
    34981 atccctcatc tcgtgaaact ttactgccca ccataggaga gcaaaacgcc gcgatcggga
    35041 tatttggatc gtcgatcgta tcaagaataa tgtctctcga ttcaaacatt accgagcgag
    35101 tgaaaaacaa actggtaaac aacacattta attttttttg cacatcttca gcattagcca
    35161 cctgcacaag ctgggcaggc gaatataaat caaccatacg catcctcttt gcattcatta
    35221 aaaataattg tggatatatg ctatcaccga tatttgctat gcgaatacat gcaaccaagt
    35281 gcaatgttgt ataaaatctg ccgtgacaac ttcagtgctg ataattcgtg ttaatgtatt
    35341 tacttccttt ggtcgggatt tatgtagcat gccggaaaat ctattttctt ccggcatttt
    35401 tttatttgca gaatttaaaa cggtatatta tcgccgtacg gatcatcatt ccccgactgt
    35461 tgttttgccc tgttcagtgc gtcagtagcc tggccctgtt gacctttttt gccgcccggt
    35521 cgcgccgttc tggcactgat tacactgtct gcgataacct gccagccctg ccgcgtttcg
    35581 ccgttctggc ctgtccactg gcttacctgc atgttacccg ccacgctcac cagttcgcct
    35641 ttgtggtgtt ttgccagtgc gtcggcctgt ctgccaaacg ccaggacgga taaccacatc
    35701 gtcgccgttc cgtcatctgc ctggctgcac ggaaggggga ccgccatact agccatcgtc
    35761 atttgtgtac ccttgctggt ggtctttaac tgcgggtcag ccaccagccg tccgtaagcc
    35821 gctatctgtg ctgtcatgct gtctgctctc cggttttaac gttgatggtt gttacctgtt
    35881 ccgcttcggc aatctcccgc tctgttagcg tggcaaaatt tgccgccgcc gtggtcatga
    35941 atgcgcttat cagttcggga tgtgctttcg cgtatccttc cccggcgttg cggtcgatga
    36001 tttttatcga cccccttaac cagtgttccg tcaaatcaag ggcgtgcgat tgtgattttt
    36061 ttgtgcgctt cgctgtcata ggctttatct cagagcaata aattaaaatt tttgtatttt
    36121 aacccttcac ctgttcacct tttgatattt tccatttaaa ttcatactgt taaggggtga
    36181 acagtttcac aaaaactatt caccaactgt tcaccactgt tcacccttga agctcaataa
    36241 acaaccaaaa aggtgaacag tgaatagttt ggtgaacagt tcataaataa ctgttcaccc
    36301 tataatatac tgatataaaa gatatttatg acagggtgaa cagtggtgaa cagttattcc
    36361 ataagtttaa tttttgctat cgtcattagt gactgataca catgatggca tccagtcttc
    36421 tgattcctcc gtcagtgtca cgtttgaacg taaaccgtgc ttcgttttcc gttttatata
    36481 ctccctgcca tattccgcca ttgccccagg catatcttta ccgaagcgcg tcagtgttac
    36541 aggtttacca aacccatgag ccctcatata agccagatag gcatgataga gatacctgcg
    36601 cgggctgaat ggcacaattt cagcattacc cactaacagg ccatcacaca ttaccgacgc
    36661 catgagatag ccgcagaagt ccaccagcga atccccctct cgctttatcg ccagtgcttc
    36721 ttcagatttc tgctgctcat ataacaggcg tctggcttcg tcctggtcag caaaccgtgt
    36781 aagcagatgg cgaatcacta ccgccagctc accttctatt ttttccgcca gcatcggatc
    36841 gcgttcgttc tccggtacaa cttccgaaaa attgaatatc acccgacgac gtgagatccc
    36901 cccgctgcgg tcactgaatg acatggcgtt attgttaacc gccagcacta ctgccggaat
    36961 acgcgttgag tagggggctt tgtgtttcgg gtcaattgcc accttgtcac cgcctgtaat
    37021 ggccttaatc cctgccccat caccagcgta gcgggtcata tccggcatga taatcagcga
    37081 aaagccaacc actaacgcac gttcccttgc atcttccagc gccttcatgc ttgctgatac
    37141 tgtattagcc ttacccgcca gcatggtgca aatctccgcc atcacgcttt taccacttcc
    37201 ccc
//
LOCUS       DAZKHK010000037        37184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKHK010000037 DAZKHK010000000
VERSION     DAZKHK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.037"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(376..666)
                     /gene="nadS"
                     /locus_tag="ACHOS5_001879"
     CDS             complement(376..666)
                     /gene="nadS"
                     /locus_tag="ACHOS5_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9776699.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(667..978)
                     /locus_tag="ACHOS5_001880"
     CDS             complement(667..978)
                     /locus_tag="ACHOS5_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9776700.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1207..2115
                     /locus_tag="ACHOS5_001881"
     CDS             1207..2115
                     /locus_tag="ACHOS5_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9776701.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATGHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2179..3120)
                     /gene="fabY"
                     /locus_tag="ACHOS5_001882"
                     /gene_synonym="yiiD"
     CDS             complement(2179..3120)
                     /gene="fabY"
                     /locus_tag="ACHOS5_001882"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP9776702.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3165..3602)
                     /gene="dtd"
                     /locus_tag="ACHOS5_001883"
     CDS             complement(3165..3602)
                     /gene="dtd"
                     /locus_tag="ACHOS5_001883"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9776703.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3599..4471)
                     /gene="yihY"
                     /locus_tag="ACHOS5_001884"
     CDS             complement(3599..4471)
                     /gene="yihY"
                     /locus_tag="ACHOS5_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP9776704.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4465..5064)
                     /gene="yihX"
                     /locus_tag="ACHOS5_001885"
     CDS             complement(4465..5064)
                     /gene="yihX"
                     /locus_tag="ACHOS5_001885"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP9776705.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANRLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5163..5948)
                     /gene="csqR"
                     /locus_tag="ACHOS5_001886"
     CDS             complement(5163..5948)
                     /gene="csqR"
                     /locus_tag="ACHOS5_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9776706.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5982..6878)
                     /gene="yihV"
                     /locus_tag="ACHOS5_001887"
     CDS             complement(5982..6878)
                     /gene="yihV"
                     /locus_tag="ACHOS5_001887"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFP9776707.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQMLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7046..7942
                     /gene="yihU"
                     /locus_tag="ACHOS5_001888"
     CDS             7046..7942
                     /gene="yihU"
                     /locus_tag="ACHOS5_001888"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP9776708.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNTEAVQHLVDKG
                     ATPAANPAQAAKGAEFIITMLPNGDLVRHVLFGENGVCESLSSDALVIDMSTIHPLQS
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7966..8844
                     /gene="yihT"
                     /locus_tag="ACHOS5_001889"
     CDS             7966..8844
                     /gene="yihT"
                     /locus_tag="ACHOS5_001889"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFP9776709.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            8860..10101
                     /gene="yihS"
                     /locus_tag="ACHOS5_001890"
     CDS             8860..10101
                     /gene="yihS"
                     /locus_tag="ACHOS5_001890"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFP9776710.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            10168..11070
                     /gene="yihR"
                     /locus_tag="ACHOS5_001891"
     CDS             10168..11070
                     /gene="yihR"
                     /locus_tag="ACHOS5_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFP9776711.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRGWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            11131..12006
                     /locus_tag="ACHOS5_001892"
     CDS             11131..12006
                     /locus_tag="ACHOS5_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9776712.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            12112..14148
                     /gene="yihQ"
                     /locus_tag="ACHOS5_001893"
     CDS             12112..14148
                     /gene="yihQ"
                     /locus_tag="ACHOS5_001893"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFP9776713.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGSS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14194..15579
                     /gene="yihP"
                     /locus_tag="ACHOS5_001894"
     CDS             14194..15579
                     /gene="yihP"
                     /locus_tag="ACHOS5_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776714.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQVGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15622..17025
                     /gene="yihO"
                     /locus_tag="ACHOS5_001895"
     CDS             15622..17025
                     /gene="yihO"
                     /locus_tag="ACHOS5_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776715.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            17093..17785
                     /gene="ompL"
                     /locus_tag="ACHOS5_001896"
     CDS             17093..17785
                     /gene="ompL"
                     /locus_tag="ACHOS5_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFP9776716.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(17888..19711)
                     /gene="typA"
                     /locus_tag="ACHOS5_001897"
     CDS             complement(17888..19711)
                     /gene="typA"
                     /locus_tag="ACHOS5_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP9776717.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20084..21493
                     /gene="glnA"
                     /locus_tag="ACHOS5_001898"
     CDS             20084..21493
                     /gene="glnA"
                     /locus_tag="ACHOS5_001898"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP9776718.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            21667..22716
                     /gene="glnL"
                     /locus_tag="ACHOS5_001899"
     CDS             21667..22716
                     /gene="glnL"
                     /locus_tag="ACHOS5_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP9776719.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            22728..24137
                     /gene="glnG"
                     /locus_tag="ACHOS5_001900"
     CDS             22728..24137
                     /gene="glnG"
                     /locus_tag="ACHOS5_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP9776720.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24249..24359
                     /gene="yshB"
                     /locus_tag="ACHOS5_001901"
     CDS             24249..24359
                     /gene="yshB"
                     /locus_tag="ACHOS5_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP9776721.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24592..25965)
                     /gene="hemN"
                     /locus_tag="ACHOS5_001902"
     CDS             complement(24592..25965)
                     /gene="hemN"
                     /locus_tag="ACHOS5_001902"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP9776722.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDAELPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26154..26663)
                     /gene="yihI"
                     /locus_tag="ACHOS5_001903"
     CDS             complement(26154..26663)
                     /gene="yihI"
                     /locus_tag="ACHOS5_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP9776723.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(26782..26948)
                     /locus_tag="ACHOS5_001904"
                     /pseudo
     CDS             complement(26782..26948)
                     /locus_tag="ACHOS5_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27245..27877
                     /gene="yihA"
                     /locus_tag="ACHOS5_001905"
     CDS             27245..27877
                     /gene="yihA"
                     /locus_tag="ACHOS5_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9776724.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28260..31046)
                     /gene="polA"
                     /locus_tag="ACHOS5_001906"
     CDS             complement(28260..31046)
                     /gene="polA"
                     /locus_tag="ACHOS5_001906"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9776725.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKEWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31410..32342
                     /gene="yihG"
                     /locus_tag="ACHOS5_001907"
     CDS             31410..32342
                     /gene="yihG"
                     /locus_tag="ACHOS5_001907"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9776726.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSSFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQEK"
     gene            complement(32383..33813)
                     /gene="yihF"
                     /locus_tag="ACHOS5_001908"
     CDS             complement(32383..33813)
                     /gene="yihF"
                     /locus_tag="ACHOS5_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9776727.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGPFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMLQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(33968..34594)
                     /gene="dsbA"
                     /locus_tag="ACHOS5_001909"
     CDS             complement(33968..34594)
                     /gene="dsbA"
                     /locus_tag="ACHOS5_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP9776728.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(34611..35597)
                     /gene="srkA"
                     /locus_tag="ACHOS5_001910"
     CDS             complement(34611..35597)
                     /gene="srkA"
                     /locus_tag="ACHOS5_001910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP9776729.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(35674..35943)
                     /gene="yihD"
                     /locus_tag="ACHOS5_001911"
     CDS             complement(35674..35943)
                     /gene="yihD"
                     /locus_tag="ACHOS5_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP9776730.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            36013..36597
                     /gene="mobA"
                     /locus_tag="ACHOS5_001912"
     CDS             36013..36597
                     /gene="mobA"
                     /locus_tag="ACHOS5_001912"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP9776731.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQESGEWFLFCPCDT
                     PYIPHDLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            36579..37106
                     /gene="mobB"
                     /locus_tag="ACHOS5_001913"
     CDS             36579..37106
                     /gene="mobB"
                     /locus_tag="ACHOS5_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP9776732.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
BASE COUNT         9018 a         9469 c         9182 g         9515 t
ORIGIN      
        1 attgggtctt catttgcata atctgaatgg tgtacccgaa aaacgtcagc atcctaacag
       61 caccaggatt taaggtgaaa tttgacatcc atcacagacc ggtcaatgct gccaccaccc
      121 ggtctttact tgctcgtcgt tataattttg ttggtaacag ttcgggaaac aaggaattcc
      181 ttccggaaca ccccggtatc atccgatagc attgacgtca atcatcagcg cgacggtgag
      241 tttttttaat cgaatcatcg gatggcgacg ttcagcacga ttacgggtca tagcaaaact
      301 cctgagcagg cggcactatg ccgctttcat aggagttctg aatgctggtg ctttctgtat
      361 ggcataacca tgcctctaac ggttaagtgc ttgcagcaca tgttgcggat cattggcaat
      421 cgctcgcagc aaagcctttg caggcccggt cggtgaacgt cgcccctgct cccagttacg
      481 caacgtgtct acgcttacgg aaataagatc ggcaaattta gattgtgaca accctgaagc
      541 gcggcgtatt tctttgattt tcatagcatc aacttcaaaa gtccgtgaag gctgtcgcgt
      601 tccttcaata atttcattca tctgggccat gctgtgagtc aaccgctcaa aaagtgcctt
      661 atccatttac cacctcgtat ttaacaaccg gagcattgct ttttcctggg gagacagatc
      721 atctttaata cccttacgat aaataagcag taatctgatc tcataatgtt taacctgatg
      781 aaagtaaata accctgacac cagcacgttt cccctttcct cctgagaccc atcgaacctt
      841 tcttaaccca ccagtttcag gtattacctc accatagtca ggattcaagg ccaggaaaaa
      901 ctgaaaacga ctgaattcat cgtcgttcag cagtttttga acatcttcag taaaaatttc
      961 cgtttcgatg aagagcataa ttatacgcca ttggcttaga gtaacaatcc tttttgtacg
     1021 tcattgacgt accattgaaa ccactagctt actgatagtg ctccagcaaa accacgtctc
     1081 tgccgggttt gtctttacat ttgcgaggcg ctttccagat ttttcacgcc cgtgaatttc
     1141 cctgtatcca tccagcaaaa acgggcgtat aaaactgtac attcatacag caaaatgagt
     1201 tctactatgg cactggaaaa aggaattgca gagttagtcg aagagtttat cgctgcgggt
     1261 cgcccctcat cgcgccaaca aagtattacg cagcgcagag agggctatat cgccagcgct
     1321 gtccttgcgg gtgagacaga aacgcgggta gatattcaga ctatcgaact tgaagggatg
     1381 accttacgaa tagtgtcacc gctgaatgcc ccgacattac tcccgacgat aatttattac
     1441 tacggtggct gttttgttag cggcgggttc gcaactcacg acaaccagct gcgccagtta
     1501 gcctattacg ggcaatgtcg gattattgcc gtccagtatc ggctggcacc ggaacatact
     1561 ttccccgccg cccatgatga tgcacaacga ggtgcagaat tagtccggca acacgccgaa
     1621 cgactcggcg ttgataaaca acgaataact cttgccggtg acagtgcggg cgggcatctg
     1681 gcgctggtaa ctgctttgcg attgaagcgt gctggcgagt ggcaaccagc acaactcatc
     1741 ttaatttatc ctatgcttga tgcgactggg catttcgaaa gctacatacg caatggtcac
     1801 gattacatca tcacacgcga cactttactg agcggctttg agatgtacct gccaggcatt
     1861 gaacgcaggc atcctgaagc cagccctatt tggcgcaatg atttcaatgg cttgccacct
     1921 gtacatatca ttaccgctga atacgaccca ctttgcgatg aaggtgaagc gttgtatcac
     1981 cgcatgacag ggcaaggagt gcaatgcacc tgccaacgct accttggcgt tatccacgga
     2041 ttttttcagt taggtggtat cagtgaggct gcgcgatcgg cgctacggga tgttgcctgg
     2101 agagcgggaa ggtaactccg gaccagggcc cagaaaaggt tttcagattg aaaacctttt
     2161 cagtcaagta aaacgcccct actcttcttc gttcccgccc tcttcatacg ggccaaatgg
     2221 cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa
     2281 gatttcgacc tgcatttgta cccgcgcttt tcgtccgcgc gccagacggt cgagatcgcc
     2341 acttaaggca ccaaggtcgg ctaccgcatg gggtttacca ctaataggtt tgctatagcg
     2401 gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcgcgca gcatcagcca
     2461 gataagcccc caaccggtga gcgtagccag cgagaataaa ctcccggcaa acagcgtgtg
     2521 atgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg
     2581 ctgaatgcgc acacccattt tttcactcag cgggatgtgt tcataccacg cttgctggag
     2641 ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg gtttaatcat
     2701 caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tcaccctgat taacaaaccc
     2761 cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc
     2821 ttcctgacgt gccaccgact ccagggtcat cgccatcagc gtgcctaacc ctttgtcctg
     2881 cacgtcggga tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacag
     2941 tcggcctacc gctaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc
     3001 atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca
     3061 gcgaaactga tagtaacgct ctaattcttc ttctgtttgt ggaacccgaa ggtgatacat
     3121 agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag
     3181 aatgtcacgg ggccatcatt gaccagcgat acctgcatat ccgcagcgaa gcgtcctgtt
     3241 tgcgtgttca tctcttgctg acggcagcgt tcgacgaaat agtcatataa cgcctctgcg
     3301 cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggca
     3361 agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc
     3421 ttgccttcgg catcgctaaa gatgcggtag ccgagtacac gctcgcacag acggtttgct
     3481 ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttca
     3541 cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc
     3601 atggttcgtc gtcttcttct tgttcagcag cttgttttaa tttgcggtat tccccgagag
     3661 tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga
     3721 gaatggggat caccgccagc acaccgtaaa tgagctgata tgatgggaac atggtgatat
     3781 aaagcgcgaa acctttcttt cctgcttcga acaggagcgc ggcgacaaac gcgccgacaa
     3841 tcgcgtcgcg gttaggtaca cggatggtag gaacgatgct gtacagcagc cagaaagaga
     3901 tccacgacaa cagcagcgga aaaatacgca acacattatc gatgacagta ttgagatcgc
     3961 tcgcccagcg cagagagagc agataggagc tgatcgccag actggcccct gccaacagcg
     4021 gccccagcgt taaaatcatc cagtacacgg caaacgagta aattttgggt cgcgctcgtt
     4081 tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat aacgccgtga
     4141 cgatcagccc gcacgccccg acggcggtca ttttgttgga attggcgaca aactgttcga
     4201 tatatcgctg gataacatcg ccagtagcag gcagaaagtt ggcaaaaatg aagtgacgca
     4261 actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa
     4321 ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt
     4381 tgtcctcatc aatgcgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacgat
     4441 gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt
     4501 ggttttatct ttcaccagaa tactagtaat gcctagccga ttagctcctt ctatattatc
     4561 ggcgttatcg tcgaaaaaga ccgtatcgtc tggtgaaaaa ccttctgcct gcaaaacatg
     4621 ctgataaatt cgtgcttcag gtttgcgcat acccaagtct tgcgacagat agatatggtc
     4681 agcagcatca cggatttccg ggtattcctc cggccagaag gtagtatgca ggcggttagt
     4741 attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac
     4801 ttccgggcgc agcgcgacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag
     4861 cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg
     4921 ctcatgctga tgaaacgcct cgcccatgtg gaaactcttc ttaagcgtag ccagtggaac
     4981 acgcgttaaa tcgctccagg cccccagcac acggttaaag tcgatatcga caatcacatt
     5041 acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta
     5101 gcgggaaagg atgggtttgg ctatgtggcc aggtgtgaag ccaggtcaac ggtcacaaaa
     5161 ttctacgcgt cttcctgggg aaggatgatt tcaatttggc tgtcttgtaa gcgtgatttt
     5221 agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt
     5281 tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcaggat atttctcgcg
     5341 tgcgccatca tcgttttcac cacgttagct tcgttaacat caaactccat caacgcgcca
     5401 tcgctctcga ttgcgcctac gcttgttacc agataatcgg cgcgaaaatc ggccacaaag
     5461 gacgccgctg aagggccaat gatcccactg ttatgggagc gcagcgttcc gccaggcacc
     5521 atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt
     5581 atccgcaaat gattatggtt aagtaacgcc cgggcaacat gctcaacggt cgtaccaatg
     5641 gtgataaata ttgttgaacc atcaggaata taatctgcca ccgcttcggc aatcgctttt
     5701 ttttcttcgg tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac gctggaagcc
     5761 cgacccgcgc caccgtgatg gcgcgtaatc agtccttgct cgcttaattt acgaatatcc
     5821 cggcgcaccg tctgcgtgga gacatccagc agatttgcca gctcatcaat attcatatac
     5881 ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt tagttcggta
     5941 aggctcatga aaattcctcg aaaaaccatc accctggcat tttatacaaa aagtgacaaa
     6001 aaagatcggg tttgatcaca gtcagggatc ccggctcgtc caccgggacg cgtgcatttc
     6061 aacgccgcta ctccgctggc gaagcggact gattccgcta aatccccacc tgttgccagc
     6121 gccaccgcca atgcgccgtg aaaaacatca cccgcacctg tggtatctac cacatcaact
     6181 ttgaaggccg gttgatgctg tcgcccgcca ttctccagcc agtcacagcc tgcactgccc
     6241 tgggtcacat agacatgacc atttgtgagc atttgtgcct gttttagggc actcgccatc
     6301 tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa aagctgcatg atcgcttaac
     6361 gccaccagtt cgctgatatc ctgcggcgta atatcgccgt ccagcacggt catcacgccc
     6421 gcctgccgcg ccagagtgaa ggcttttttc gcgccgtcgt gccagcgtac atctgccagc
     6481 accacgtccc actgagagaa atcaatttcc tccaaccact ctgcatcagg cagcaggtct
     6541 gggctgggat agttaatgat gatgcgctca cctttggcat ccaccatgat ggcggattgc
     6601 gaagatttcg cctggttata ccgtttggtg taacgggtgt taacacccca ggattccagc
     6661 tctgccagca ggctgttgcc cgtgtcgtcg tcaccgacgc gaccaataaa atcgacctgc
     6721 gcacctagtc tggccgccgc aactgctgct gtagccgctg gcccgccgcc aacttccgta
     6781 taatttttcg ccacgtattt accgctctcc gtcggcaatc cttccacgta atagatgcga
     6841 tccatcacgg taattcctac acaagcaaca cgaatcatgg tcattcctta agcattttgg
     6901 gatgcgttca ttttaatttc atcaattgtt taaaaagtga cggctgtcaa ttttttgacg
     6961 ataaattaca aatacgatca aaaacagaca agcaaggcat gtcataaatg acaaaaagtg
     7021 acatcactgt attcaggaga gagttatggc agcaatcgcg tttatcggtt tagggcaaat
     7081 gggttcgcca atggcgagca atttattgca gcaagggcat caactttgcg tcttcgatgt
     7141 gaataccgaa gccgtacagc atctggtcga caaaggggcg actcccgctg ctaacccagc
     7201 gcaggccgct aaaggtgccg aatttatcat taccatgttg ccgaatggcg atctggtgcg
     7261 tcacgtgttg ttcggtgaaa acggtgtctg tgagagctta tcttccgatg cgctggtcat
     7321 tgatatgtcg accatccacc cactgcaatc cgataaattg attgctgata tgcaggccag
     7381 aggcttcagc atgatggatg ttccggtagg ccgtacttcc gcaaatgcca ttaccggtac
     7441 tctgctactg ctggctggcg gcactgctga acaagttgaa cgcgccacgc cgatcctgat
     7501 ggcgatgggc agcgagttga tcaacgcggg cggtccgggc atggggatcc gcgttaagct
     7561 tatcaacaac tacatgagca tcgcgctcaa tgcgttgtcg gcagaagccg ccgttttgtg
     7621 cgaagccctc aatcttccct tcgatgttgc cgtcaaggta atgagcggta ctgccgccgg
     7681 taaaggccac ttcaccactt cctggccgaa caaagtcctc agcggagatc tttctcccgc
     7741 cttcatgatc gatcttgccc ataaggatct tggcatcgcc cttgatgtcg ccaaccagtt
     7801 gcatgtgccg atgccactgg gggccgcctc acgggaggtt tatagccagg cgcgcgcagc
     7861 gggtcgcggt cgccaggact ggtccgccat tctggagcag gtccgtgtca gtgccgggat
     7921 gactgccaaa gtaaaaatgt aacgactgga taaaggaata aatgaatgaa taagtacacc
     7981 atcaacgaca ttacgcgtgc atcgggcggt tttgccatgc tggcggtcga tcagcgcgaa
     8041 gccatgcgca tgatgtttgc cgcggctggc gcacccgctc cagtagccga tagcgtttta
     8101 actgatttca aagttaacgc cgcgaagact ctttcacctt atgcatcggc gattctggta
     8161 gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg cgattgccaa aagttgcgcc
     8221 atgattgtcg ccgccgatga gttcattcct ggcaacggta ttccggtaga tagcgtggtc
     8281 attgaccgca aaatcaatcc gctacaaatc aaacaagacg gtggcaaagc cttaaaactg
     8341 ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgcc tggatatggt gaaagagttt
     8401 aacgaaatgt gccactcaca cggtctggta agcatcattg agccagtcgt ccgcccaccg
     8461 cgtcgtggcg ataaattcga tcgcgaacaa gcaatcatcg atgcagccaa agagctgggc
     8521 gacagtggcg ctgatctcta caaagttgaa atgccgcttt acggtaaagg tccgcaacaa
     8581 gagcttctca gtgcttcaca acgtctgaat gaccatatca atatgccgtg ggtgatcctc
     8641 tcttccggtg tcgacgaaaa actgttcccg cgtgccgtac gcgtggcaat gacagcgggt
     8701 gcatcgggct tcctggctgg tcgtgccgtc tgggcatcgg tcgtcggttt accagacaac
     8761 gagctgatgc tgcgtgacgt ttgcgcaccg aaattacaac aacttggcga tatcgtcgac
     8821 gaaatgatgg ctaaacgccg ttaagatggg gatacaaaaa tgaaatggtt taacacttta
     8881 agtcacaacc gttggctgga acaggaaacc gatcgcatct ttgattttgg taaaaaatcc
     8941 gtagtaccga ccggatttgg ctggttaggc aataaagggc aaatcaaaga agagatgggc
     9001 acccatctgt ggatcacagc tcgtatgttg cacgtttatt ccgttgctgc ggcgatgggc
     9061 cgacctggcg cttactcgtt ggttgatcac ggtatcaaag ccatgaatgg cgcactgcgc
     9121 gataaaaaat atggcggttg gtatgcctgc gtgaatgacg agggcgtggt ggatgcctcc
     9181 aaacagggct atcaacattt ctttgctctg ctgggtgctg ccagcgccgt cactaccggt
     9241 catccggaag cgcgcaagct gctcgattac accattgaaa ttatcgagaa atacttctgg
     9301 agcgaagaag agcagatgtg cctggagtcc tgggatgaag ccttcagcaa aaccgaagag
     9361 tatcgcggcg gcaatgccaa tatgcacgcg gtggaagcct tcctgattgt ctatgacgtc
     9421 acccatgaca aaaaatggct ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac
     9481 gtcgccagaa ataatcatta tcgcgttaac gaacacttcg atacccagtg gaatccgctg
     9541 ccagattaca acaaagataa cccggcgcac cgcttccgcg cgttcggagg tactccgggc
     9601 cactggatcg aatggggccg tttaatgctg cacattcacg cggccctgga agcccgttgc
     9661 gaacaaccgc cagcatggct gctagaagat gccaaaggtc tgtttaacgc caccgtgcgc
     9721 gatgcctggg cacccgatgg cgcggacggg attgtttata ccgttgactg ggaaggaaaa
     9781 ccggtggtcc gcgaacgtgt gcgttggcct atcgtcgaag cgatgggtac ggcctacgcg
     9841 ctctacaccg tcactggcga tcgccagtac gaaacctggt atcagacatg gtgggactac
     9901 tgcatcaaat acctgatgga ctacgaaaat ggttcatggt ggcaggagct ggatgcggac
     9961 aataaggtca ccaccaaagt ctgggacggc aaacaggata tttatcatct gctgcattgt
    10021 ctggtgatcc cacgtatccc gttagcgcca ggcctggctc cggcggttgc ggcgggtctg
    10081 ctggatatta acgcgaaata actgactaaa aaaacgacat atcaggctgc acgctgggta
    10141 tgtcgttatt caataaaggt acctcgtatg caaatcacca acatgcattg cagtggacag
    10201 accgtttcac ttgccgcggg ggattatcac gcgaccattg tgaccgtcgg tgcaggtctg
    10261 gctgaattaa cctttcaggg ctgtcattta gttattccgc ataaacccga agagatgccg
    10321 ctggcacatc tgggcaaagt ccttattccg tggccaaacc ggatcgccaa cggctgttat
    10381 cgctatcagg ggcaggaata tcagttaccc attaatgaac atggttcaaa agccgctatt
    10441 catgggctgc tcgcctggcg tggctggcaa atcagcgaat taagcgccac cagcgtcaca
    10501 ttaaccgcct ttctgccacc cagttacggc tacccgttta tgctggcttc gcaggtggtt
    10561 tattcgctca acgcccgcac gggtctttcc gttgagatcg caagccagaa tatcggcact
    10621 gtcgcagcgc cttacggtgt gggtattcac ccttatttaa cctgcaactt aacatctgtt
    10681 gatgaatatc tgttccagct tccggcaaac caagtttatg ccatcgatga gcacgctaat
    10741 ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt tcacgcaggc gaaaaaaata
    10801 gccgcgacaa aaatcgacca cacctttaaa accgctaatg atttgtggga aatgactatt
    10861 acccatccac agcaggcgct ttcggtgagc ctctgttccg atcaaccctg ggtgcaggtt
    10921 tatagcggtg aaaaattaca acgtcaggga ctggcagttg aaccaatgag ttgcccgcct
    10981 aacgccttta attcaggtat cgacttatta ttactggaac cagggaaaac gcaccgcctg
    11041 ttttttaata ttcacgggca gcacaattaa ttttattaag aaaagaacga gatcattgtt
    11101 tagagttgta tttacacaac aggaaatatt atggactttt ttacgttatt ttcaccggat
    11161 ggggtcatgc tggaaacaaa cgtggtatgt atcgttatcg cgttaatgtt tctttatacc
    11221 tttgtgggga tatgcgcggg atttggcggt ggtttaacca ccatgccgct cgtcacgtta
    11281 atgctccccg taaaaatggc aactccgctc tcggtgattg tcggcaccgc gaccgccttg
    11341 tacgccacct ggctgtcacg taaagaaacg gattggcgat cggcaatggt actgattatt
    11401 ttctcgtttc tgggaatacc cgtcggttta tatgcgctgt cgtatttacc cgaccatttt
    11461 atgaaagtgg gcttaggcgg attccttatc ctgtactcct tttacagtat gtttattcca
    11521 cgcctgccga tttatgaccg tagctggatc gccgcaccta tcggcgccgt ggcaggagcg
    11581 ttaggggcag cattttcaac caacggaccg ccgatggtta tctacggtat gctgcgtaac
    11641 ttaggcccgg cggcatttcg cggcactctg aacgctttct tcaccgcaaa caacatcgcg
    11701 gtgatcggcg ggctggcgac cagcggaatt ttgactgtct ctatctttaa actggtgctg
    11761 ttttgtattc ccaccatgat cctcggttca ctggttggac aatatgtgca taaacgcatt
    11821 tcggtcaaag tgttccgtgt gatggtcttt ttattgctga ttgcctccgg ttctatgttg
    11881 ataaaaggcg cgctgggaat tcccgccgct gccgcacttc ttcctccgtt taccttgtta
    11941 gttatactgc aactggcatt tggtaaaaaa gtggcggcaa tccgtcgggc tgaagaaagt
    12001 aaataatcat tatgcgggtt ttaatttata cccgcacaca ttctttaatt aatgaaacct
    12061 gtaataacag gatggcatga aatattagtt ttatatctat ggagattatt tatggatacg
    12121 ccacgcccac agctattaga ttttcaattt catcagaata acgacagttt tactctacgt
    12181 tttcaagacc gccttatttt aatccatagc aaagataatc cttgtttatg gattggttca
    12241 ggtatagcgg atatcgatat gttccgcggt aatttcagca ttaaagataa attgcaggag
    12301 aaaattgcgc ttaccgacgc catcgtcagc cagtcaccgg atggctggtt aatccatttc
    12361 agccgtggtt ctgacattag cgccacgctg aatatctctg ccgacgatca tggccgttta
    12421 ttgctggaac tacaaaacga caaccttaac cacaaccgca tctggctgcg ccttgccgca
    12481 cagccggaag atcatatcta cggctgcggc gaacaattct cctactttga tctgcgcggc
    12541 aaaccgttcc cgctatggac cagtgaacaa ggcgttggtc gcaacaaaca aacctatgtc
    12601 acctggcagg ccgactgcaa agagaatgcg ggaggggatt actactggac cttctttcca
    12661 caacccacgt ttgttagcac gcagaagtat tactgccatg ttgataacag ttgctatatg
    12721 aacttcgact ttagcgcgcc ggaatatcat gaactggcgc tgtgggaaga caaagcaaca
    12781 ctgcgttttg aatgtgctga tacatacatc tccctgctgg aaaaattaac cgcgctgctg
    12841 ggacgccagc cagaactgcc cgactggatt tatgacggag taacgctcgg cattcagggc
    12901 ggcactgagg tgtgccagaa gaaactggac accatgcgta acgcgggcgt gaaggtcaac
    12961 ggcatctggg cgcaggattg gtccggtatt cgtatgacct cgtttggcaa acgcgtaatg
    13021 tggaactgga agtggaacag cgaaaactac ccgcaactgg attcacgtat caagcagtgg
    13081 aacaaagagg gcgtacagtt ccttgcctat atcaacccgt atgttgccag cgataaagac
    13141 ctctgcgaag aagcggcaaa gcgcggctat ctggcaaaag atgccgccgg cggcgattat
    13201 ctggtggagt ttggcgagtt ctacggcggc gttgtcgatc tcactaatcc ggaagcctac
    13261 gcctggttca aggaagtgat caaaaagaac atgattgaac tcggctgcgg cggctggatg
    13321 gctgacttcg gcgagtatct gcccaccgac acttacctgc ataacggcgt cagcgccgaa
    13381 attatgcata acgcctggcc tgcgctgtgg gcgaaatgta actacgaagc ccttgaagaa
    13441 acgggcaagc tcggcgagat cctcttcttt atgcgcgccg gttccaccgg tagccaaaaa
    13501 tattccacca tgatgtgggc gggcgaccag aacgttgact ggagtctgga cgatggactg
    13561 gcgtctgtcg tcccggcggc gctgtcgctg gcaatgaccg gacatggcct gcatcacagt
    13621 gatattggcg gttacaccac cctgtttgag atgaagcgca gcaaagagct gctgctgcgc
    13681 tggtgcgatt tcagcgcctt cacgccgatg atgcgcaccc atgaaggtaa ccgtcctggc
    13741 gacaactggc agtttgacgg cgacgcggaa actatcgccc attttgcccg tatgaccacc
    13801 gtcttcacta ccctgaaacc ttacctgaaa gaggccgtcg cgctgaatgc gaagtccggc
    13861 ctgccggtta tgcgccctct gttcctgcat tacgaagacg atgcacagac ctacaccctg
    13921 aaatatcagt acctgttagg ccgcgacatt ctggtcgccc cggtacacga agaaggtagc
    13981 agcgactgga cgctctatct gccggaggat aactgggtcc atgcctggac gggtgaagcg
    14041 ttccggggcg gggaagtgac cgttaatgcg cccatcggta aaccgccggt cttttatcgc
    14101 gccgatagcg aatgggcggc actgttcgcg tcgttaaaaa gcatctaatc acatccgccc
    14161 gtggggaaac tcacgggcaa agggagaaca acgatgagtc acatcacaac ggaagatcca
    14221 gcaactctac gcctgccctt taaagagaaa ctctcttacg gtatcggcga tctggcctct
    14281 aacatcctac tggatatcgg cacgctttat cttttgaagt tttataccga tgttctgggg
    14341 ctgcctggca cctatggcgg cattatcttt ttgatttcaa aattctttac agcctttacc
    14401 gatatgggta ccggcattat gttggattcc cgacgcaaga tcggtccaaa aggtaagttc
    14461 cgtcctttta ttctgtatgc gtcatttccg gtcaccctgc tggcgatcgc caactttgtc
    14521 ggcacaccgt ttgatgtcac cggtaaaacg gtgatggcca ccatcctgtt tatgctttac
    14581 ggactgtttt tcagcatgat gaactgctcg tatggcgcga tggtccccgc tattactaaa
    14641 aaccccaacg aacgtgcatc gctggcagca tggcgtcagg gtggcgctac attgggcctg
    14701 ctgctgtgta cggtgggatt cgtgccggtt atgaatctta tcgagggtaa tcagcaactt
    14761 ggctatatct tcgccgccac gctgttttca ctgttcggcc tgctgtttat gtggatctgc
    14821 tattcgggcg tgaaagagcg ttatgtcgaa acccaaccta ccaatccggc gcaaaagcct
    14881 ggcttgttgc aatctttccg cgcaattgcg gggaaccgcc cactgttcat tttgtgcatt
    14941 gccaacctct gtaccttagg ggcgtttaac gtcaagctcg ccattcaggt ctattacacc
    15001 cagtacgtgc ttaacgatcc catcctgttg tcatatatgg gatttttcag catgggctgt
    15061 attttcatcg gcgtgttcct gatgcctggt gcagtcagac gttttggtaa gaagaaggtc
    15121 tatatcggcg gtctgctgat ctgggtgctg ggcgatctgc tcaactattt cttcggcggc
    15181 ggctcggtca gctttgtggc tttctcctgc ctggcgttct ttggttcggc gtttgttaac
    15241 agcctgaact gggcgctggt ttctgacacc gtcgagtatg gcgagtggcg caccggtgtg
    15301 cgttcggaag gtacggtcta caccggtttc accttctttc gcaaagtatc tcaggcgctg
    15361 gcaggtttct tcccaggctg gatgctgacg caagttggct atgtgccaaa cgtcgcccag
    15421 gctgaccaca ctatcgaagg gttgcgccaa ctgatcttca tctacccaag cgcactggca
    15481 gtagtcacca ttgtggcaat gggttgcttc tacagtctga acgagaagat gtacgtccgc
    15541 attgttgaag agatagaagc ccgtaaacgc acggcgtaat tataataaac aacgccctgc
    15601 ggggcgttat aaggagtgat tatgtctgac cataatccac tgacattaaa actgaatctg
    15661 cgggaaaaaa tcgcctatgg tatgggcgac gtcggttcga atttaatgct tagcatcggc
    15721 actctgtatc tcctcaaatt ttataccgat gaattaggga tgcctgctta ctatggcggg
    15781 attatctttc tggtggcgaa gttttttacc gcatttaccg atatgctcac cggattttta
    15841 ctcgactcgc ggaaaaatat tggacctaaa ggaaaattca ggccgtttat tttatacgca
    15901 gcgattccgg cggcgttaat tgcaacgctc cagtttatcg ccaccacctt tagtttgccg
    15961 gttaaaacga cgattgccac cgcactgttt atgatgtttg gactttcata cagtctgatg
    16021 aactgctcgt atggcgcgat gatcccggcc attaccaaaa acccgaatga gcgcgcgcaa
    16081 ctcgctgctt accgccaggg tggtgcaacc atagggctat tgatctgtac cgtggcgttt
    16141 attcctttgc agtcactatt ttctgactcc accgtcggct acgcctgcgc ggcactgatg
    16201 ttctccattg gcggctttat ttttatgatg ctgtgctaca gaggtgtcaa agagcattat
    16261 gtggacacga cgccaaccgg acataaggcc agtattctca aatcattttg cgcgatattt
    16321 cgtaatccac ctttgctggt attgtgcatt gctaacctgt gtaccctggc ggcatttaat
    16381 atcaaactgg cgattcaggt ctattacacc cagtatgtgc tgaatgatat taacttattg
    16441 tcgtggatgg gattctttag tatgggatgc attctcgtcg gcgtattact ggtgccacta
    16501 actgtaaaat gttttggtaa aaaacaggtt tatctggctg gcatggtgct gtgggcggtg
    16561 ggggatatcc tgaattattg ctggggcagt aactcattca ctttcgtcat gttctcttgt
    16621 gtcgcctttt ttggcacggc ctttgtaaac agcctgaact gggcactggt accagatacc
    16681 gtagattatg gtgaatggaa aacaggtata cgtgccgaag gttctgttta taccggttat
    16741 accttctttc gtaaaatttc agccgcactt gctggcttct tgccaggcat tatgctgacg
    16801 caaatcggtt atgttcccaa catcgcgcaa agcgatgcaa cattacaggg tttgcgtcag
    16861 ctcattttta tctggccttg tgcgctggca attattgctg cattgacgat ggggttcttt
    16921 tacacgctca atgagaaacg gtttgcactg attattgagg aaattaacca acgcaaaaat
    16981 aaagaaattg agacagaaga aaaaacggct tccgtaacgt tataaatgtc aatagccgct
    17041 atttccatcc tgatgggtgg cggcctccct acgtttaaaa aatggactta ttatgaaaaa
    17101 tattaatgca ataattttat tatcttctct gacctctgcc tcggtattcg ctggcgcata
    17161 cgtcgaaaac agggaagcat ataatctcgc ctccgatcag ggtgaggtta tgctacgtgt
    17221 gggttataac ttcgatatgg gcgcgggtat tatgctaacc aatacctaca cctttcagcg
    17281 agaagatgaa ctcaaacatg gctataatga aattgaaggt tggtatcctt tatttaaacc
    17341 taccgataaa ttaaccatcc agcccggtgg cttaattaat gacaagagta tcggttcggg
    17401 tggtgctgtt tatctggatg ttaactacaa atttactccg tggtttaacc tgacggtgcg
    17461 taatcgctat aaccataata attacagctc tacagatttg aacggcgaat tggataataa
    17521 cgacacctat gaagttggca cctactggaa ctttaaaata acagaaaaat tttcctatac
    17581 atttgaacca cattacttca tgcgagtgaa tgactttaat agtagcaacg ggaaagatca
    17641 tcattgggaa atcactaaca ccttccgtta ccgtattaat gaacactggc ttccttattt
    17701 cgaattgcgc tggttagacc gcaacgtcga gccataccac cgcgagcaaa accagattcg
    17761 tatcggaacg aagtatttct tctgatgtaa aaattcctga tgcgatgctg gcgcatctta
    17821 tcaggtctac aaaagccaaa aaaaaacctg ccatcgctgg caggtttttt atgactaaaa
    17881 aacgaaatta atcgtctttc ggtgcgcggt tggcgcgacg acgatcgttt tccgtcaggt
    17941 gacgtttacg aatacgaata gaggtcggag tcacttctac cagttcgtca tcatcgatga
    18001 actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg gcttcgtcag
    18061 taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca gtcaggtcgt
    18121 tagagcggct gtgaataccg ataatctgac cttcgtaaac ttcagcaccg tgaccgagga
    18181 acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc taccgcttta ccctgaccgt
    18241 tagagatcag tacgccgttc tgacgctggc ccacttcacc cggacgtacg tcgtcgtagt
    18301 ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac tcagaacgga
    18361 agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct ttaccgtctg
    18421 gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattaca gaaccctgat
    18481 gctgttcttc aacgtccagc gtcacgtttt catacggctc ttgtttacga ccgtcgattt
    18541 cacggaagat aactttcgga cgggataccg ccagttcgaa accttcacga cgcatgtttt
    18601 cgatcagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca tcggcgtctt
    18661 cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga cgatccagga
    18721 tctgacgaga tgttacgaac ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga
    18781 agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca acgttttgcg
    18841 tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc cgtgatcgcc acgatatcgc
    18901 cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc actttaccga
    18961 ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt aacctgctgg ttcggcttca
    19021 ctttaccgcg cttgatgcgg ccaatgccga taacgccaac atagctgttg taatcgagct
    19081 gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga acgtggtcaa
    19141 caatcgcctg gtacagcggg gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga
    19201 taccgttcag cgcagaagcg taaacgatcg ggaagtccag ttgctcgtcg gtcgcgtcga
    19261 ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg ccagggcggt
    19321 caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt ttggttacga
    19381 agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact gagtctacca
    19441 tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta tcaacgatgt
    19501 tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta atcccacgct
    19561 ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga gagtcgaacg
    19621 taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct acgtgcgcga
    19681 tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta gaaatagcgc
    19741 gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct ccgcaaacaa
    19801 gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc
    19861 attgcaccaa tatggtgctt aatgttccca ttgaagcact atattggtgc aacattcaca
    19921 tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg ggcaatttaa
    19981 aagttggcac agatttcgct ttatcttttt tacggcgaca cggcccattt aattgcagat
    20041 ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
    20101 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa
    20161 aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgccgaat tcttcgaaga
    20221 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgatat
    20281 ggtgctgatg ccagacgcat ccaccgcagt gattgatccg ttcttcgccg attctaccct
    20341 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
    20401 ctctattgcg aagcgcgctg aagattacct gcgttctact ggcattgccg acaccgtact
    20461 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat cgtctatctc
    20521 cggttcccac gttgctatcg acgatatcga aggtgcatgg aactcctcca cccaatatga
    20581 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
    20641 agactcagct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt
    20701 agttgaagct catcaccacg aagtcgctac cgcaggtcag aacgaagtgg ctacccgctt
    20761 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgtcg tgcacaacgt
    20821 ggcgcaccgt ttcggtaaga ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
    20881 ttccggtatg cactgccaca tgtcgctgtc taaaaacggc gtcaacctgt ttgctggcga
    20941 caaatacgca ggtctgtctg aacaggcact gtactacatt ggcggcgtaa tcaaacacgc
    21001 taaagcgatt aacgccctgg caaacccgac caccaactct tacaagcgtc tggtcccggg
    21061 ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat
    21121 tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg acccggcggc
    21181 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggca tcaagaacaa
    21241 gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa
    21301 agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg atctggaccg
    21361 cgagttcctg aaagctggtg gcgtgttcac ggatgaagcg atcgatgcgt acatcgctct
    21421 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt ttgagctgta
    21481 ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg atttagttgc
    21541 cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc tctgatgctt
    21601 cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg ttcaggagac
    21661 tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc gctgattaac
    21721 agtattttgt taatcgatga caatctggcg atccattacg ccaaccccgc cgcgcaacaa
    21781 ctgctcgccc aaagctcccg caaattgttt ggtacgccgt taccggaact gttgagctac
    21841 ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg ttttaccgat
    21901 aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac ggctcagcgt
    21961 atgccggacg gcatgatcct gctggagatg gcaccgatgg ataaccagcg ccgcttaagt
    22021 caggaacagc tacagcacgc ccagcaggtt gctgcccgtg atttagtgcg cggcctggca
    22081 catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctact cagcaaagca
    22141 ttacctgacc cgtcactact cgaatatacc aaagtgatta tcgaacaggc ggaccggctg
    22201 cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcat taccgaaagt
    22261 attcacaaag tggctgaacg cgtggtgacg ctggtgtcga tggaactgcc gaacaacgtg
    22321 cggttgattc gggattacga ccccagcctg ccggaactgg cgcacgaccc ggatcaaatt
    22381 gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc ggagggtggt
    22441 gaaatcattc tacgtacccg cacagcgttt caactgacct tacacggcga gcgctatcgg
    22501 ctggcggcgc ggattgatgt ggaagataac gggccgggca ttccgcctca tttgcaggat
    22561 acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg cttatccatc
    22621 gcccgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg gccaggtcat
    22681 accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg caacgaggga
    22741 tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt gcgctcgctg
    22801 gagcgggttt aacctgtacg acatttgaga acggcgcgga agtactggag gcgctggcga
    22861 gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac gggctggcgc
    22921 tgctcaagca gattaaacag cgccatccga tgcttccggt catcattatg accgcacatt
    22981 ccgatctgga tgctgccgtc agcgcctatc aacaaggggc atttgattat ctgcccaaac
    23041 cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc catcagtcat taccaggaac
    23101 agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc ggtgaagcgc
    23161 cagccatgca ggacgtgttc cggattatcg gtcggctttc gcgttcttct attagcgtgc
    23221 tgattaacgg cgaatccggc accggtaaag aactggtcgc tcatgccctg catcgccaca
    23281 gtccgcgagc caaagcgccg tttatcgcgc tcaatatggc ggctattccg aaggatttga
    23341 tcgaatccga actgtttggt cacgagaaag gcgcatttac cggcgcgaat accattcgtc
    23401 aggggcgttt tgaacaggct gatggcggta cattatttct cgatgaaatt ggcgatatgc
    23461 cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt tatcgcgttg
    23521 gcggctatgc gccggtgaaa gtagatgtgc ggattatcgc tgccactcac cagaatcttg
    23581 aacagcgggt gcaggaaggc aagttccgtg aggatctgtt ccaccgcctg aacgtcatcc
    23641 gcgttcatct gccgccactg cgcgaacgtc gggaagatat tccccgtctg gcacgccatt
    23701 ttttacaggt tgccgcgcga gagctgggcg tagaagcgaa gttgctgcat ccggaaaccg
    23761 aagccgcgct gacgcgtctg gcgtggccag gcaacgtgcg ccagctggaa aacacctgtc
    23821 gctggctaac ggtgatggcc gccgggcagg aagtgttgat tcaggatttg cctggcgaac
    23881 tgtttgaatc aaacgtaccg gaaagcactt cacacatgca gccggacagt tgggcaacgc
    23941 tgttagcaca gtgggcagac agagcgctgc gttccggtca tcaaaacctg ctttccgaag
    24001 cacagccaga gctggaacgg acgttactga ccaccgcgct gcgacatacg caggggcata
    24061 aacaggaagc ggcgcggcta cttggctggg gtcgcaacac cctgacgcgt aagttaaaag
    24121 agctggggat ggagtgattc acggcttgtg tgtaaagatt gattattgag cgcaaattgc
    24181 tggtattttg cgctttactg ttccgataag ttcagtatga tcttgcccgg aaaacgggga
    24241 gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt gacagccaca
    24301 caccgcaaac tgctgttgct gccgtgctgt gtgccgcgat gatagggctg tttagctgaa
    24361 gctcgttgtt tacgccggat gcggagaaag tcgtaggcct gataagacgc aaccagcgtc
    24421 gcatcaggcg ttgattgccg gatgcggcgt aaacgcctta tccggcctac aaaagcaata
    24481 atttcaacag attttgcagg cctgataagc gtagcgcatc aggcaatctt gttctcagca
    24541 cattgatggc gctttcgttt tctactttgt aaacgaagcg ccattcacaa tttaaatcac
    24601 ccgagagaac tgctgcatcc gcgctttctg gcgcagatag gtatcaaagc acatgcaaat
    24661 gttgcggatc agcaagcgac ctttcgccgt cacctgtatt cccttctcat ccacatccac
    24721 cagcccatct tttgctaacg gggcgagcaa cttgagatct tccgcaaagt aatcagcgaa
    24781 gagcaaatcc cactgttgct caataggggc gtaatccaga cggaagttgc agatgagcga
    24841 cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc gcaataccac gccacagcgc
    24901 attgccttgt tcatccactt gctgatagta ctgcttcaac tctttctggt tctgcgcgta
    24961 gcagtcgcca atcatgctga tggcggaaac gcccatcccc agcagatcgg tatcgccctg
    25021 agtggtgtag ccctggaagt tacgatgcag cacgccttca cgctgggcca ccgccagctc
    25081 gtcatccgga cgggcaaagt gatccatacc gataaactga tagcccgatt gtgtcaggaa
    25141 ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga ctcggcagtt cagcgtcttt
    25201 gattttgcgc tgagcagcaa aaatggtcgg caaatgcgcg tagttaaaga cgctcagacg
    25261 atcggggttc agctccgcca cgcgtttcag ggtaaaggcg aaactctccg gcgtctgttt
    25321 cggcaggccg taaatcaggt cgatgttggt ggaggtaaag ccaatctcac gcgcatggtt
    25381 aagcagtgca aagatgaact cttcatcctg ctcgcggtta accagacgtt gcacttcttt
    25441 gttgaagtcc tgcacgccca tgctcagccg attaaagcct tcggcgcgta aatgatcgag
    25501 tacatccagt tcgatttccc gcggatcgac ttcgatcgag atctccgcat cggcattgaa
    25561 ctggaagttt tcgcgcagca gcttcatcag acggctgatt tgcgctttat tcagatacgt
    25621 cggcgttccg ccgccccagt gcaattggct gacgtgacgc ccggcgaaca gcggtgcacg
    25681 atggacgatt tcttgctcca gcgcgtccag atactgatcg gccttgtgct gctggcgagt
    25741 aacaatctta ttgcaaccgc agaagtaaca aagcttatgg cagaacggaa tatgtacgta
    25801 gagagataat ggacgctcag gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa
    25861 gtcttctgaa aactccagcg cggtcgggta cgaggtgtat cgtggcccgg aatagttata
    25921 tttctggatc agggccagat cccagtcgat ttgctgtaca gacatgctca ctccttccga
    25981 tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt ttcgagtcat cagccggttg
    26041 cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa cgaataacca caccagataa
    26101 catataaccg gaagggttat cagcagaacg ggaaggccca cggcggcaat ccgttagttg
    26161 ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg
    26221 tcataagaga ggccgagttt ctgcatcaac tcatcaatac gatccagttt ggcatccacc
    26281 caggattgct cttcggcgct cagggtttcg cctgcttcca gacgttccag cagcgcatcc
    26341 agacgctcat ccgtttccag taactccaac tccgcctgcg gtgaaagcat aggtttctca
    26401 ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc ccaatggaat aggggtttta
    26461 ctgccaatac gtggatcttt tggtgcgttc tgacctttgc tgcctgacgt ggtgttaccg
    26521 cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt tctgacgctt acggtcacga
    26581 gcttcctgat ccagctcctc gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt
    26641 gagtttgaag atgatggttt cataatggtt cgtcttttag ccgttttatt cagtatagat
    26701 ttgcggcgga atctaacaga aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc
    26761 ttttttcctg actcataacc cttaacgggt cttacaatcc ttgcaggccg ccaacacacc
    26821 ctgtttttcc attagccgga acaatccgtg ttgattccat ttccatttaa ttacgtctcc
    26881 ggacgtttat cttcctgaca atcctctgtc ttcgcctcct ggcgctcctg accctcatcc
    26941 tgagtcattg ttcctgttag cgtcctcgct ctatgggtgc tactttacgc ctttgcttta
    27001 aataaacaat caaccgccat cagtatttgc acattttcag agttttatat aaaagcaatt
    27061 cactgattta tatagcttat ttgacatacg tcgcagaatt ttctctgcta tttcgcataa
    27121 agactatcgg caatgtctca caaagcatat ggcttttact tacaacgctc acgccgaaaa
    27181 atccgccttt tattatggtt caggtataat cgccgcaaag ctttgactga tggagacgac
    27241 cgctttgact aatttgaatt atcaacagac gcattttgtg atgagtgcgc ctgatattcg
    27301 ccacctacct tccgataccg gaattgaagt ggcttttgca ggccgttcca acgcaggtaa
    27361 atccagcgcg ctgaacacgc tgactaacca gaaaagcctg gctcgtacct caaaaacccc
    27421 ggggcgcacc cagcttatca acctatttga agtggctgac ggcaagcgtc tggttgactt
    27481 gcctgggtac ggttatgcgg aagtcccgga agagatgaag cgcaaatggc agcgcgcgct
    27541 cggcgagtac ctcgaaaaac gtcagagcct gcaaggtctg gtggtgctaa tggatattcg
    27601 ccatccgctg aaagatttgg atcagcagat gattgagtgg gcggtagaca gcaatatcgc
    27661 cgtgctggta ctgctgacca aagcggacaa actggcaagc ggcgcacgta aagcgcaatt
    27721 gaatatggtg cgtgaagcgg tgctggcgtt taacggtgat gtgcaggttg aaacgttttc
    27781 ttcgctgaag aaacaaggcg tggacaagct gcggcagaaa ctggatacct ggtttagcga
    27841 gatgcagcct gtagaagaaa cgcaggacgg cgaataattt tcttgcctta atgcttgtgc
    27901 cggatgtggc gtatccggcc cgtaaattca atagattaca attctaccgt tggcctgata
    27961 aacatggcgt atcaggcatt acggatcttt tctttccgcc caataaaaaa cgccccagtc
    28021 attactgact ggggcggcta aaatattcag ccaaatccga ttacgtgaag taaaaggtct
    28081 gaaagataga acatcttacc tctgtaccct acgcgactaa ctttactctt ttttgttcag
    28141 ttcagaaagc attttttgta attttttttc agttgttgca taggaatttc taatagccat
    28201 cacaaaacgt gacagcttat gttgcttact tacgaaaaaa gggcatgttc aggcgaatct
    28261 tagtgcgcct gatcccagtt ttcgccactc cccacttcca ccagtaacgg cacatccaga
    28321 cgggtacagt tttccatcag ttgatgaatc tgcttcgcga cggcatcgac atcatcttta
    28381 tggacttcaa ataccagttc atcgtgtacc tgcatgatca tacgcacacg cggttgctca
    28441 gcctgtagcc acgcatcaac ggcaatcatc gcccgtttga taatgtcggc ggcggttccc
    28501 tgcattggcg cgttaatggc tgcacgttca gccgctgcac gacgagcacc attgctggat
    28561 ttgatatccg gcagatacag acggcgtccg tccagcgttt caacgtagcc ctgctctttc
    28621 gcctgagcac gggtgcgttc catatactcc agcacgccag ggtagcgttc gaagtaaagg
    28681 tccatgtact tctgcgcttc tttacgcgga atgttcaatt gccgcgccag accgaaagca
    28741 ctcatgccat aaatcagacc aaagttgatc gctttcgcgc tacgacgttg ctcgctggtg
    28801 acggtttcca gtggcaaacc aaacacttct gccgccgttg cacggtggat atcttttcct
    28861 tcggcgaatg cggtcagcaa gcctttgtca cgcgagagat gcgccataat gcgcagttca
    28921 atctgcgagt agtccgctga gacaatcaca taatcctctg gcgcaataaa cgcctggcgg
    28981 atacggcgac cttcttcgtt acgcaccgga atgttttgca ggttaggatc ggttgacgat
    29041 aaacgtcccg ttgcggttac tgcctggtga taagaggtat gtacacgccc ggttttcggg
    29101 ttgatcatca acggcagctt gtcggtgtag gtcgatttca gcttcgccag accacgatac
    29161 tccagaatca cttttggcaa cggatagtcc agcgccagtt cttccagtac ctcttccgac
    29221 gttgacgggg cgccacccgg cgttttcttc agtggtttaa tgccctgttt ttcaaacaga
    29281 atggtttgta actgcttggt ggaagaaagg ttaaattctt cacctgcaat ttcatgagct
    29341 ttcttttcca gctcagccag acgcaaggtg agctcttcag agtgattgtg cagcactttc
    29401 ggatcgatct tcacaccgtt acgttcaatg cgtgaaagca ccggcaccag cggcatttcg
    29461 atattctcga agacgttcaa cggccctttg tgtttttgca gatccggcca cattttcaga
    29521 tgcaactgca aggtgacatc tgcatcttcg gcggcgtaac gtccggcttc ttcgagggca
    29581 atctggttaa aggtcagttg atttttgcct ttacccgcaa tctcttcaaa agtgatggtt
    29641 ttgtgcttca accaacgttc cgcgaggctg tccatatcgt gacgtccggc aacgctattg
    29701 agaatgtagg actccagcat ggtatcaaac gcaatcccac gtagttcaat gccgtagttc
    29761 gccagaatac cgcgatcgta tttcaggttt tgcccgacct tcagcgcctt ttcatcttcc
    29821 agcagcggtt ttagcaactc gagtgcacgc tcgcgagaga tttgatcggg cgcatcaaga
    29881 taatcatgag caaccggaat atatgccgct acgcctggct cgatagcaaa agaaagcccg
    29941 accaggttag cagagatgtt atcaaggctg tcggtttcgg tatcaaatgc aaataccggc
    30001 gctttttcca gcttcgcaat ccactctttc agtgtttctt catcaaggat ggtgacgtag
    30061 ttgtcataag aaatcaccgt tgccgtcact tctggcgctt cgtctgcaac actggtttcc
    30121 tgcggcctgg ctgcgggttt cgccccttta gcctgtagcc attttcccgc ttcgacatca
    30181 gcagtccagc gtttgaactc atactttttg aacagaccta acaactcttc cgccgccggt
    30241 ggctgcactt ccagttgttc acaggtcagc tccagttcaa cgtcggtttt aatcgtcgcc
    30301 agctggtatg agagataagc aacttctttg ttttgctcga gcttcgctgc cattgttttc
    30361 gcgccacgga agctcaaccc agcaattttt tctggctcgg catacagcgt atccagcccg
    30421 ccaagacctt gcagcaatgc ctgcgcggtt ttttcaccga cgcccggtac gccaggaatg
    30481 ttatcagagg agtcacccat cagcgccagg aaatcgatga tcagttctgg cggcacgccg
    30541 tacttattca ccacctcttc cggtccgagg atggtattcg tcatggtgtt gataagcgtg
    30601 atatttggtg tcaccagctg cgccatatct ttatcgccag tgctgatcag caccggacgc
    30661 ccggcttttt cggcttcgcg cgccagagta ccaataacgt cgtccgcttc tacgccagaa
    30721 accgccagca gcggcagtcc catcgcttta accatcgcgt gcagaggttc gatttgtgct
    30781 cgcagatcgt ccggcattgg cggccgatgt gatttgtaat gttcaaacag ttcatcacga
    30841 aaggtttttc ccttggcgtc aaagaccacc gctgcatgcg tcggtttata ttgcatgatc
    30901 agactgcgca gcatgttgag gacaccatac atcgcaccgg taggctcgcc tgcgctgtta
    30961 gtcagcgggg gaaacgcgtg atatgcgcga taaagataag atgaaccatc tacaaggata
    31021 agtggatttt gggggatctg aaccataatg tccgtgcctg tttatcagat tatgggtaaa
    31081 ggatgccaca gacaggatga aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt
    31141 agatcttctg gatcgctcgc aaatcgtcat gtggataact ttgtgcataa attatttaca
    31201 tcgcttttaa tttattatca tgctttttac agcatggtgt ttgtctttgt ttttcatgca
    31261 gttaaaaata aagccatccc taataattga gataatggga ttttttctaa tgtggataga
    31321 tatgaattat ttttctcctt aaggatcatc cgttatttgg gtcgttttct gctaaaatct
    31381 gcgccttgcg cttctttagc gcactgtcta tggctaacct tttgaataaa ttcattatga
    31441 cgagaatact cgctgcgata acccttttgc tgagtatcgt attgaccatt ttggtcacta
    31501 ttttttgttc cgtcccgatc atcattgccg ggattgtaaa actattgcta cctgtgccag
    31561 tcatttggcg aaaggtttcg cgtttttgtg attttatgat gtattgctgg tgtgaaggtc
    31621 tggcggtatt actgcatctg aacccacacc tgcaatggga agttcacggg ctggaagggt
    31681 taagtaagaa gaactggtat ctgcttattt gtaaccaccg tagttgggca gatattgtcg
    31741 tactgtgcgt gctgtttcgt aagcacattc cgatgaataa atatttcctc aagcagcagc
    31801 tggcctgggt gccttttctt ggcctggcgt gctgggcgct ggatatgccg tttatgaagc
    31861 gttattcccg cgcttattta ttacgtcatc cggagcgacg tggtaaagat gttgaaacca
    31921 ctcggcgttc ttgtgaaaag tttcgcctgc accccaccac tattgtcaat ttcgttgaag
    31981 gctcccgttt cacgcaagaa aaacatcagc aaacccactc ctcttttcaa aacttgttgc
    32041 caccaaaggc ggcaggcatt gcgatggcgc taaatgtact gggtaaacaa ttcgataaac
    32101 tgcttaatgt tacgctgtgt tatccggaca ataaccgtca gccgttcttc gatatgttaa
    32161 gcggtaaact gacgcgaatt gtcgtacatg tggatttaca gcctattgcc gatgagttac
    32221 atggcgacta catcaatgac aaaagcttta agcgccattt tcagcaatgg ctgaactcat
    32281 tgtggcagga aaaagaccgg cttctgacat cgttgatgtc atcacagcgg caggaaaagt
    32341 aagttttact cgcctctgaa tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc
    32401 ggcacgagac ataaattctt cttcgctcat ctcctgtccg ttaaaaacaa ctttacccgg
    32461 tgtataacgc agttgcagcg aggcggtatt gttgtcaatc gtgattaact gcaacatctg
    32521 acctaatgtc atcatcccgc tgatttgttt atcagcccgc ttttgcgctt tttccgcatc
    32581 catcccttca gataaattaa gctgttttgc ggtttctgta gcgacattaa gcggtaactt
    32641 catatcaaaa ttgagcgatt taatatcttt gtttgtggat gatgaagatt tggctgggtc
    32701 ggcaatactg atatccagat tggcatttag ttcgccgagt gcgttcttcc atcttactgg
    32761 ttgtttaatc gtcggctcac ttttttgtaa taacggtaaa tactctttga acaatgcagc
    32821 attcacttct tgcagagcaa cttcatcgtt tgctaactca gggtgtgcag caagctgctt
    32881 ctgcatcgca atgttatact ggatgataaa ttggcgcacc gctgagggat caatagattc
    32941 agcaatcaat gaccactcac cactaccgag ttgctgattc ccttttgtta acttatcaag
    33001 ttcgtaggtc agattgacat tgatataatc tttgtcttta acgcgatcca gcctggtttt
    33061 tgcaacgaaa gcatcgattt gggcaacgac ttccccctgg ttgataatat taacctgatt
    33121 aaatttcgac tcgctttcac caaccggaaa tttcttttct tctaatcgtg tcagagaatc
    33181 aatggtaaaa cttttcgccg ttactttgtt cattggtgaa aattgcagaa caatatcttc
    33241 gactttgcct tcaatatcca ggttagacaa tgaactatca tcaccattaa aagtaaactg
    33301 gccttcacca aaagaaaact ttccatactc tgcaggggca acatctaatg tcgtggagaa
    33361 tgggccacca aacgcgaatc gcaatgtcgc ttccacgggc gatttatttt tcgcggcgat
    33421 aaatagcggt tgtgtcagct cattattcac taagttcact tttgccgctg ccagtgctgg
    33481 gatgacatta ccatgcatta acattgtgat gggcaacgga ccgtgctcaa catccacatc
    33541 aaatacaatt ttctggcctg gcttgatatt gagatcgggt gcaccgttat caaatgtcat
    33601 ttgcatttga aaacgggaat taaaaaagcc tttgtcaaaa tttttataac ttaacgtcat
    33661 atcgtaggca tgttcacctt tctttttagc atcgttaaaa cctttaataa acttttcgac
    33721 accaggctga atttgcgtac ctgtgtacca tgtgccacca ccccagatta cggctaacgc
    33781 aacaatggca cctgtagctg acttacgtat catataaata ttccctgata aaacagcaaa
    33841 actttgaata tccacttatg ctgaaataat gtccagcagc aggaagcatt atcagaatgt
    33901 atttatattt attaaataat aaaaaaagcc cgtgaatatt cacgggcttt atgtaattta
    33961 cattgaatta ttttttctcg gacagatatt tcacagtatc agcatactgc tgaacaaaaa
    34021 catccatatt gctggtatcc ataccctgcg gattcagctg atatttaccg ttaacaaaca
    34081 tcgccggaac accacgcaat tgcacgtcag ctgcagcttt ttcctgctga gcgaccagag
    34141 atttcaccac gaagctgttc cacgccgcgt cgtactcttc acctttaata cctgcgttga
    34201 taaatacatc gcggatatca gatgctgaac gaatggtctg ggttttttgt acgccttcaa
    34261 acagcggaac tgtgactttg tcttccacgc ccaacgccat cgccacagcc catgcctgag
    34321 tcagctcttt gcccaggtca ccccccatga agttgacgtg gtatttagtc atcttcacgc
    34381 cttccggcag ttttttcttc acgttatcag aaatatgcag aacttcttca aactgatagc
    34441 agtgcgggca gaagaaagag aaaaactcca gcacttgcgg cgcgccagca actggttttt
    34501 ccagggtagt gtactgttta ccatcttcat actgcgccgc cgatgcgcta aacgctaaaa
    34561 ctaaaccagc cagcgccagc caaatctttt tcatgatcta ctctctccga ttaatacata
    34621 ggtgttaatt gcaaaggggg ttcttgtaga acttttgcct gttctataaa agtcgccgtc
    34681 tgtcgtagcc agtaatcttc cccggttaac cacgggaaat ttttcgggaa cgcgggatca
    34741 gcccaacgcc gcattagcca ggcaagataa taaaccaaac gcatggcgcg taaaggttca
    34801 atcagtccga tttcagcggt gtcgaactcg ctaaattctt cataagcctc aataatagtt
    34861 tccagttgca tccgctgctc ggctttatca ccattgagca acatccacaa atcctgaacg
    34921 gctggaccat tacgtgcatc atccagatca acaaacattg gaccatcgcg ccagagaata
    34981 ttcccggcgt ggcagtctcc atgtagccgc agaacggtga aatcttcccg ccagtgtgct
    35041 gtaacggcgg caatcagctc atctgtcgct ttcaggaatg ccgctttcaa cccggaaggt
    35101 attagtgtag cgtcctcaaa cagcttgcgt ggctcaatga gatattcatt caaaccgatg
    35161 gtcgggcgat ggataaaaag ctgtttgcgc cccgtctggt gcatacgccc taaataacgc
    35221 ccaaccgctt ccatctgatc gatattatca gcttcgaact ggcgaccacc gacgcttgga
    35281 aaaacagcga aataaaatcc ctgatggttc aataaagtct gaccattaaa ggccacaggt
    35341 gctgcgaccg gaacttcatc gttaaccagc tgcaacgcaa attgatgttc ttcgaggatt
    35401 tgatcggctg tccaacgttc agggcgataa aatttgacga caaaacgtcg acgatcttcg
    35461 tcctgaaatt gatagacacg gttttcatag ctgttaagcg gggtaagacc ggaatccacc
    35521 cgaatcccat gctcaaacag agcgtccatg atggtatccg ggtgtagtgt ctggaaagta
    35581 aaagcgctgt tgttcatccc atcatccgga aaacattacg aatgatgaag gatatcattt
    35641 cacgatgatt ttggttgcgc cgcttacaag cttttactct ttaattacgc cacgtgcgcg
    35701 taatagcgcc gttttgaaat cttcttcata atctttctgc aaacccggga tcaccgcatc
    35761 tttcgcggaa tcacgcattt tcaggtgata gatcagaatg tcatccgtca aatccgccag
    35821 ttcgccgtca aaacctgact ctttcgccag tttctgcaaa aattgcagca ggttaaggtc
    35881 cggctctttt tgccaggctg gctggaggag ttcaataact tcattcagac gtttacattt
    35941 catagtaatg ctccttactc ttgagacaga cacgttagca gggtcaatcc cacaataaaa
    36001 gaggcgatat cggtgaatct gatgacgacg ataacaggcg ttgtgctggc gggcggtaaa
    36061 gccagacgaa tgggcggcgt agataaagga ttgcttgaat taaacggcaa accattatgg
    36121 caacatgtcg ctgacgcgct tatgacgcag ctttctcacg tcgtggttaa tgctaatcgt
    36181 catcaggaaa tctatcaggt aagcggtctg aaagtgattg aagattcact ggcggattac
    36241 cctggccctc tggcaggaat gctttcagta atgcagcagg aatcgggtga gtggtttttg
    36301 ttttgcccgt gcgatacgcc ttacattccc catgatttag cctcccggct taatcatcag
    36361 cgcaaagatg cgcctgtcgt gtgggtccac gacggtgaac gcgatcaccc gactattgct
    36421 ctggtaaacc gcgcaattga gcctttatta ctggaatatc tgcaagcagg agaacgccgg
    36481 gttatggcat ttatgcgtct ggctggcggt catgcggttg atttcagcga tcgtaaagag
    36541 gcatttataa acgtgaatac gccagaggag cttgcccgat ggcaggaaaa acgatgatac
    36601 cgttactcgc ctttgctgca tggagtggca ccggaaaaac aacgctgctg aaaaaattga
    36661 tcccggcatt atgcgccaga gggatccgtc cggggctgat taagcatacg caccatgata
    36721 tggatgttga taaaccgggc aaagatagct atgagctgcg caaggctggc gcggcgcaaa
    36781 ccatcgttgc cagccaacag cgatgggcct tgatgacgga aacaccagac gaagaagagc
    36841 tggatctaca ttttctcgca agtcgaatgg atacctcaaa gctggatttg attctggtcg
    36901 aagggtttaa gcatgaagag atcgcaaaga ttgtgctgtt tcgcgatgga gccggacatc
    36961 gaccggaaga attagtgata gacaggcatg ttattgctgt agccagtgat gtgccgctta
    37021 atcttgatgt cgcgttactg gatattaatg atgttgaggg gctggctgat tttgtggtgg
    37081 agtggatgca aaagcaggac ggataagcaa gagtaggctg gagcaaacgc gccagcgtcg
    37141 catccggcat ttattccgca ccaacgcaaa agagcccagt cttc
//
LOCUS       DAZKHK010000038        36346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKHK010000038 DAZKHK010000000
VERSION     DAZKHK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.038"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..585)
                     /locus_tag="ACHOS5_001914"
     CDS             complement(<1..585)
                     /locus_tag="ACHOS5_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9776733.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGF"
     gene            complement(656..2770)
                     /gene="fusA"
                     /locus_tag="ACHOS5_001915"
     CDS             complement(656..2770)
                     /gene="fusA"
                     /locus_tag="ACHOS5_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9776734.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2867..3337)
                     /gene="rpsG"
                     /locus_tag="ACHOS5_001916"
     CDS             complement(2867..3337)
                     /gene="rpsG"
                     /locus_tag="ACHOS5_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9776735.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3434..3808)
                     /gene="rpsL"
                     /locus_tag="ACHOS5_001917"
     CDS             complement(3434..3808)
                     /gene="rpsL"
                     /locus_tag="ACHOS5_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9776736.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3934..4221)
                     /gene="tusB"
                     /locus_tag="ACHOS5_001918"
     CDS             complement(3934..4221)
                     /gene="tusB"
                     /locus_tag="ACHOS5_001918"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP9776737.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIISIDYTDFVRLTVKHSSQMAW"
     gene            complement(4228..4587)
                     /gene="tusC"
                     /locus_tag="ACHOS5_001919"
     CDS             complement(4228..4587)
                     /gene="tusC"
                     /locus_tag="ACHOS5_001919"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP9776738.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4587..4973)
                     /gene="tusD"
                     /locus_tag="ACHOS5_001920"
     CDS             complement(4587..4973)
                     /gene="tusD"
                     /locus_tag="ACHOS5_001920"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP9776739.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4973..5695)
                     /gene="yheO"
                     /locus_tag="ACHOS5_001921"
     CDS             complement(4973..5695)
                     /gene="yheO"
                     /locus_tag="ACHOS5_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9776740.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5862..6674)
                     /gene="fkpA"
                     /locus_tag="ACHOS5_001922"
     CDS             complement(5862..6674)
                     /gene="fkpA"
                     /locus_tag="ACHOS5_001922"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9776741.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6895..7113
                     /gene="slyX"
                     /locus_tag="ACHOS5_001923"
     CDS             6895..7113
                     /gene="slyX"
                     /locus_tag="ACHOS5_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP9776742.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7162..7752)
                     /gene="slyD"
                     /locus_tag="ACHOS5_001924"
     CDS             complement(7162..7752)
                     /gene="slyD"
                     /locus_tag="ACHOS5_001924"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9776743.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7847..8047)
                     /gene="yheV"
                     /locus_tag="ACHOS5_001925"
     CDS             complement(7847..8047)
                     /gene="yheV"
                     /locus_tag="ACHOS5_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP9776744.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8057..9833)
                     /gene="kefB"
                     /locus_tag="ACHOS5_001926"
                     /pseudo
     CDS             complement(8057..9833)
                     /gene="kefB"
                     /locus_tag="ACHOS5_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9833..10384)
                     /gene="kefG"
                     /locus_tag="ACHOS5_001927"
     CDS             complement(9833..10384)
                     /gene="kefG"
                     /locus_tag="ACHOS5_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP9776745.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10515..12428
                     /gene="yheS"
                     /locus_tag="ACHOS5_001928"
     CDS             10515..12428
                     /gene="yheS"
                     /locus_tag="ACHOS5_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9776746.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQNRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12428..13450
                     /gene="yheT"
                     /locus_tag="ACHOS5_001929"
     CDS             12428..13450
                     /gene="yheT"
                     /locus_tag="ACHOS5_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9776747.1"
                     /translation="MAQITTTDANEFSSSAKFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13444..13662
                     /gene="yheU"
                     /locus_tag="ACHOS5_001930"
     CDS             13444..13662
                     /gene="yheU"
                     /locus_tag="ACHOS5_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP9776748.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13716..14585
                     /gene="prkB"
                     /locus_tag="ACHOS5_001931"
     CDS             13716..14585
                     /gene="prkB"
                     /locus_tag="ACHOS5_001931"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP9776749.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14640..15044)
                     /gene="yhfA"
                     /locus_tag="ACHOS5_001932"
     CDS             complement(14640..15044)
                     /gene="yhfA"
                     /locus_tag="ACHOS5_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9776750.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15346..15978
                     /gene="crp"
                     /locus_tag="ACHOS5_001933"
     CDS             15346..15978
                     /gene="crp"
                     /locus_tag="ACHOS5_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP9776751.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            16029..18119
                     /gene="yhfK"
                     /locus_tag="ACHOS5_001934"
     CDS             16029..18119
                     /gene="yhfK"
                     /locus_tag="ACHOS5_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP9776752.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVVVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVATRL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18186..19406)
                     /gene="argD"
                     /locus_tag="ACHOS5_001935"
                     /gene_synonym="dapC"
     CDS             complement(18186..19406)
                     /gene="argD"
                     /locus_tag="ACHOS5_001935"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP9776753.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPVSAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGAMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVVGA"
     gene            complement(19492..20055)
                     /gene="pabA"
                     /locus_tag="ACHOS5_001936"
     CDS             complement(19492..20055)
                     /gene="pabA"
                     /locus_tag="ACHOS5_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP9776754.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(20087..20689)
                     /gene="fic"
                     /locus_tag="ACHOS5_001937"
     CDS             complement(20087..20689)
                     /gene="fic"
                     /locus_tag="ACHOS5_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP9776755.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQVAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20679..20846)
                     /gene="yhfG"
                     /locus_tag="ACHOS5_001938"
     CDS             complement(20679..20846)
                     /gene="yhfG"
                     /locus_tag="ACHOS5_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP9776756.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEDLRRHYER"
     gene            complement(20951..21523)
                     /gene="ppiA"
                     /locus_tag="ACHOS5_001939"
                     /gene_synonym="rotA"
     CDS             complement(20951..21523)
                     /gene="ppiA"
                     /locus_tag="ACHOS5_001939"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP9776757.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21794..22975
                     /gene="tsgA"
                     /locus_tag="ACHOS5_001940"
     CDS             21794..22975
                     /gene="tsgA"
                     /locus_tag="ACHOS5_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP9776758.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23237..25780
                     /gene="nirB"
                     /locus_tag="ACHOS5_001941"
     CDS             23237..25780
                     /gene="nirB"
                     /locus_tag="ACHOS5_001941"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP9776759.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQSKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25777..26103
                     /gene="nirD"
                     /locus_tag="ACHOS5_001942"
     CDS             25777..26103
                     /gene="nirD"
                     /locus_tag="ACHOS5_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP9776760.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            26229..27035
                     /gene="nirC"
                     /locus_tag="ACHOS5_001943"
     CDS             26229..27035
                     /gene="nirC"
                     /locus_tag="ACHOS5_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP9776761.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            27054..28427
                     /gene="cysG"
                     /locus_tag="ACHOS5_001944"
     CDS             27054..28427
                     /gene="cysG"
                     /locus_tag="ACHOS5_001944"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP9776762.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28679..28846
                     /gene="yhfL"
                     /locus_tag="ACHOS5_001945"
     CDS             28679..28846
                     /gene="yhfL"
                     /locus_tag="ACHOS5_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP9776763.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(28954..30039)
                     /gene="yhfS"
                     /locus_tag="ACHOS5_001946"
     CDS             complement(28954..30039)
                     /gene="yhfS"
                     /locus_tag="ACHOS5_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9776764.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVIGRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVIHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETILRILRESIASI"
     gene            complement(30051..31355)
                     /gene="yhfT"
                     /locus_tag="ACHOS5_001947"
     CDS             complement(30051..31355)
                     /gene="yhfT"
                     /locus_tag="ACHOS5_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP9776765.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(31367..31720)
                     /gene="yhfU"
                     /locus_tag="ACHOS5_001948"
     CDS             complement(31367..31720)
                     /gene="yhfU"
                     /locus_tag="ACHOS5_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP9776766.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(31731..32609)
                     /gene="php"
                     /locus_tag="ACHOS5_001949"
     CDS             complement(31731..32609)
                     /gene="php"
                     /locus_tag="ACHOS5_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP9776767.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGVLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(32606..33832)
                     /gene="yhfW"
                     /locus_tag="ACHOS5_001950"
     CDS             complement(32606..33832)
                     /gene="yhfW"
                     /locus_tag="ACHOS5_001950"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9776768.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            complement(33833..34996)
                     /gene="yhfX"
                     /locus_tag="ACHOS5_001951"
     CDS             complement(33833..34996)
                     /gene="yhfX"
                     /locus_tag="ACHOS5_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP9776769.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVSDAVEQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHRGEPEIVGRYDSLGNPLEA"
     gene            complement(35080..35442)
                     /gene="yhfY"
                     /locus_tag="ACHOS5_001952"
     CDS             complement(35080..35442)
                     /gene="yhfY"
                     /locus_tag="ACHOS5_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9776770.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(35459..>36346)
                     /gene="yhfZ"
                     /locus_tag="ACHOS5_001953"
     CDS             complement(35459..>36346)
                     /gene="yhfZ"
                     /locus_tag="ACHOS5_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP9776771.1"
                     /translation="KKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAAL
                     KTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEGLASGLK
                     AQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALELGPHTY
                     VGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESLQRIVKG
                     DVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVILTRADDYPMQQLLRAVVDK
                     HALLAHQQRVVSGEQEPSY"
BASE COUNT         8830 a         9897 c         9042 g         8577 t
ORIGIN      
        1 gaagccagcc agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag
       61 agcagaacca cgaacgatcg gagtgtcgtc gcccgggaag tcgtactgag acagaagttc
      121 acgaacttcc atttcaacca gttccagcag ctcttcgtca tcaaccatgt cgcatttgtt
      181 caggaacacg atgatgtacg gaacgcctac ctgacgaccc agcaggatgt gctcacgagt
      241 ctgcggcatc gggccgtcag tcgcagcaac taccaggatc gcgccgtcca tctgagcagc
      301 accggtgatc atgtttttaa catagtcggc gtgccccggg cagtctacgt gcgcgtagtg
      361 acgggtcggg gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc
      421 ttccggcgcg ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag
      481 tacggtggtg attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc
      541 aacgttaacg tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac
      601 tatagtgctc tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt
      661 taccacgggc ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga
      721 attccatagt gtatgatgcg cgacctttgg tcagagaacg cagctgagtt gcatatccga
      781 acatttcaga cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac
      841 ctttgagcat accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttcag
      901 gagtttctac ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc
      961 cttctttaaa ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat
     1021 ggtaagaacc gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac
     1081 ctgctttcag ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta
     1141 caccaccttt aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg
     1201 ggtacatgtc gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt
     1261 taccttcaac atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac
     1321 ctacgttcgc ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca
     1381 gttcgcccat acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag
     1441 acgggtcttc tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg
     1501 ttttcggttc aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga
     1561 tcggcgcatc cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgattgcag
     1621 ctgcgatgtc gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa
     1681 cgatacgacc gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt
     1741 taaccacacc ggagtacaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga
     1801 ttttgaacgc cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt
     1861 ctttaccgtc gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat
     1921 caattaccgc atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta
     1981 ccaggatgat ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag
     2041 tcagttcttc accacccaga tatttttcca tcagctcttc agaagcttca gctgcggatt
     2101 cgatcaggtt ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt
     2161 attcgaaggt tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt
     2221 caacaacacc ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg
     2281 cgcccagacg ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca
     2341 ttttgttaac gaacgcaata cgcggaactt tatatttgtt tgcctgacgc catacggttt
     2401 cagactgcgg ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac
     2461 gcatggaacg ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga
     2521 tgcgatgcgg ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag
     2581 tgatggtaat accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat
     2641 gaacttcacc gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg
     2701 ttttaccggc gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg
     2761 tacgagccat ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg
     2821 gctgcttact ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt
     2881 gtgcgaacgc cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag
     2941 tacctttgtt ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat
     3001 caccgcgttt acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg
     3061 gacggacttc aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta
     3121 cagtcgggcg cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc
     3181 gctgagccag ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat
     3241 ctaccatcag gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca
     3301 gaattttacg ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca
     3361 ggattgtcca aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact
     3421 taacggagaa ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac
     3481 ggtctttaac gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga
     3541 ggtctttaac acgaccacca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt
     3601 caccaccgat gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac
     3661 gcagcgcgga gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt
     3721 tttgcgggca tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt
     3781 tgcgtaccag ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt
     3841 aaacacgtaa taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga
     3901 aaaggtgtca agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt
     3961 aaccgtaagt ctgacgaaat cagtatagtc aatggaaatg atgtcgttcg aaattcgacc
     4021 agtcaaaccg cgggcaataa ggtcttcgtt cagagcatag accttaatgg gggcattacg
     4081 cagactttca aggtagcggt tgccgtcaac tgcggcagtt acgccatctt gcaataatag
     4141 cagttcgtca ccttcactga gcagacgcag cagcgcagca aagtccgtca gccagggtga
     4201 acgatgtaat gtgtgcagca taacgcctca aaacctcaaa ataacatcgt agttggcgag
     4261 ttcgcggcgt aaggcatctg cttcgagcgg cgtggcttcg accacaaagg gtgtctgcgg
     4321 atctaaccca cgttcgcgca gtgaagccgc gcaaatccag cactgttcaa tatcgtacag
     4381 acccaacaat ttaaaagtgg caatgtaatc acgcgccagc actgcatcgg gcttttgtcc
     4441 tggcagcagc tgaaaaacgc catcagcaat aaagaagact gccagatcgt cagttaatgc
     4501 ggaagttgcc agtaaagcat ctaaaccttc ccggcctgca gttgtaccat gaggcgcagt
     4561 agaaaaaaca aacgcaattc gtttcatcag aactgtacca ccctgtcaca ggtcagcgag
     4621 gcttccgcca gcgcgccaag tccgcttaag gtaaatcccg gctgaaggtt tgacgaagcg
     4681 agccccagtc ttccggcctc cgtttcatca acaacgccac ggcgtaatgc tgccgctacg
     4741 cagatattca gcgccacacc atgttgcgca ttcaactgtt gccagccgcg cacaaggtca
     4801 aattcgtcgc ttgccggtga ggtcaattgg ttagcgttat agaccccttc ccgatagaaa
     4861 aaaacgctgc ttaactcatg gccttctgct atcagcgcct gcgcaaactg aaaagcacta
     4921 ctcgcctgtt gcgtaccgta tgctggcccg gtcaccacga tggcaaaacg cattacttat
     4981 cttgcccctg gaaatcaccg ctcttgaact ggcggatgta gagatagaca gtgtgtttgg
     5041 agatgttcag gcggtcagca acctggttga tcgcgtcttt aatatcgaag atccctttct
     5101 cgtagagatt tagcacgatc tgacgatttt tggcgttatt agaaacattg cgatcggcat
     5161 tcacttcttc gatggtgaac tccagcgttt gggtaaccag atcttcaaca gaagaggcaa
     5221 agttgacgct tgaaccgaca tccggagttt ccggcggcac aaaagtgctc ataatctgcg
     5281 agaagggaac atcaagattc atattgatgc acagcagacc aattacacgc tgttcgcggt
     5341 tacgaatcgc gatagtcagg gacttcatta atacgccgct tttggcgcga gtaaagtagc
     5401 atttagaaac gctgctatcc gctcccgtca tatcgtgcag catacgtagc gccaggtcag
     5461 taattggcga accaatcttc cggcctgtat gttcaccgtt agcaatgcga atggctgaac
     5521 attttagatc ctgcaaagag tgcaaaacga tttcacagtg ggagccaata agcatcgcta
     5581 acccgtccac caccgcttcg taggatttca gaatatcaaa atcggtctgg tcaaaaggac
     5641 gttgatccag taaatccaac tcactggttt cgttggttaa aagcgacctg gacatgaaaa
     5701 aaaacactcc ttttcaggag cctgtcgtta acttttcagg gcaggctcat taatgatgcg
     5761 ggtaactaaa ttaatacagc ggaggttccg ctttccagca ctaattatat ccggcctgta
     5821 ataaaaaaac cgccgcctgg tcaggcggcg gttcttagtg cttatttttt agcagagtct
     5881 gcggctttcg catcagcttc cggctttgca tcagccttcg gcgctggttt cacatccagc
     5941 agctctacgt caaacaccaa ggtagaattc ggtgggatcc ccggaacccc cgctttgccg
     6001 taagccagtt ctggtggaat aaccagtttg atcttaccgc ctttcttgat gttcttcaga
     6061 ccttctgtcc aacccgggat aacaccgtcc agacggaaag agagcggttc accacgagtg
     6121 taagagttgt cgaactcttt accgtcgatc agcgtacctt tgtagttcac gacgacagta
     6181 tcgctgtctt tcggtgcttc gcctttaccg gcttctacta cctgataaac cagaccagtg
     6241 gaagaggttt tcacaccttt ctctttggca aatttctcgc ggtactcttt accttttgct
     6301 tcgttatcag ccgcgtcttt ttccatcttc gcctgagcag aagacttcac gcgagcttcg
     6361 aatgcttgca gagtctgttc gatctcttgg tcggaaagtt tgctcttatc agcaaatgca
     6421 tcctgaacac cagcgatcag ctgatcttta tccagtttga tgcccagttt ttcttgttct
     6481 ttaagagagt tttccatgta acgacccagc gaagcaccca gtgcataagc tgatttctga
     6541 tcgtcatttt tgaacgctgc tttgctgtca gcagttgtag caggttttgc agcttcagca
     6601 gcaaaagtga ttggtgcatg cagggcaacg gccattgtgg tcgccagcag cgttacttta
     6661 aacagtgatt tcatccatat ctccaggatc ggggcatctc accccagggt taactattat
     6721 cagaagggta ctataaagcg ttgtcgaaca aatctacata cagacacgcc ctattatcat
     6781 ctattttcag actctttttg tttaaattag tttcgatgac cgcgaaatga gtgctgtctc
     6841 gggcagcaaa gttaagtaga atccgcggcg accattcgac aaaagaggtg aatcatgcag
     6901 gatttatcat tggaagcacg cctggcagag ctggagagcc gactggcttt tcaggaaatc
     6961 accattgaag aactgaacgt cacggtgacc gctcatgaaa tggaaatggc gaaactgcgc
     7021 gatcatctgc gtctgctgac cgagaagtta aaagccagcc agccgtcgaa catcgcgtcg
     7081 caggctgaag aaacgcctcc gccacattat taaggcgtaa aaaaagcggg gattccccgc
     7141 ttttttgtca ccttttcggt attagtggca accgcaaccg ccgttgcctt taccgccaca
     7201 gcagccttcg ccaccgtgtt catgaccgtg atcgtggcca tgaccaccgc agcaaccatc
     7261 gtggtcgtga tcgtggtggt gatcgtgcgc gccgtgaacg tgaccatgag ccagttcttc
     7321 ttcggtcgct tcgcgaatcg ccacaacttc aacgttgaat ttcaggttct gaccagccag
     7381 catgtggtta ccatcaacca ctacgtgatc gtcttcaacc gcagtgattt caaccggtac
     7441 cggaccctgg tcggtttcag ccaggaaacg catacctacc tgcagttcat caacgcccat
     7501 aaatacgtct ttaggaacgc gctgcaccag gttttcgtcg tactgaccgt aagcgtcgtt
     7561 cgcgccaaca gcgacatcaa atttgtcgcc aacttcatga ccttccagcg ccgtttccag
     7621 gccagagatc agggaaccgt gaccatgcag gtagtccagc ggcgcactca ccggagactc
     7681 atcaaccaac acaccgtctt ctgtacgtac ctgataggcc aggctgacca ccaggtcttt
     7741 tgctactttc atgatatctc ctgagcatgg gaagaatagt ggcgcagatt gtagcggaat
     7801 tctgcagccg tgtactcact agcttaaaaa aactcggcgc atatcgctag tccggatgaa
     7861 aaatcccgat cacttgctca tctttgcgga cgtgatcgcg ggcttctttg tctgcttctc
     7921 gcatctgatg tccacactta acacattcaa cgatatcaat attattttcg cgccacatcg
     7981 ccattgaatc ctgtgcctga caggccgggc attttgcgcc cgcaataaaa cgttttcgga
     8041 ttgccatctt taccctctac tcaaattcat cccagccgtc cagctggcgt cgttcttgtt
     8101 gcatttcacg ctggaaaatc tcttccagtt cacgtcgggc ttccctggcg cgagaaattt
     8161 gtacggtatc agcatgcatc gggattaact cccgcagcat tcgcatatcc aggcggcgaa
     8221 aatgcagttg cgcgcgctgc gcctgatgcg gatgcatgcc aagcgtgacc agcgtcttgc
     8281 gccccagctc taacgcactg gagaatgttt cacgggaaaa ctgcgtcacc cctgcctgta
     8341 ataactcatg cgcttccaca cgtccgcgcg ctcgcgcaag aatatgcaaa tgcggaaagt
     8401 gctgctggca tatttccacc agcttcatag tgtcttccgg ctcgttacag gtaatgacga
     8461 tagactcagc ggcctctgca cccgcagaac gtaaaagatc tacctgcgtg gcgtcgccgt
     8521 aataaacttt gtagccatat ttgcgcatca ggttaacggc actgatatcc cgctccagta
     8581 cagtaatgcg cattttattt gccatcagca aacgaccaat cacctgacca aaacgcccaa
     8641 agcccacgac aatgacctgc ggtttatcat cgttgaccca cggtttttca tcctcttctt
     8701 ccggtccgtt aaactggcgg gatagccatt tatctaccag cttcatcagc agcggcgtgg
     8761 tcatcatgga aagcgtcacc gtcaccagta acagcgccat ctggtcgcca tggaataagc
     8821 gttgtgaaga agcggtagaa aagagcacaa aggcaaactc accgccctga ctcaacacgc
     8881 cagcaaactg catccgctca gaactgcgca cgccatacaa tcgcgccagc agatacagca
     8941 cgagaatttt caccgccacc agcacaacca cacttatcac tacccacaac agatgggtat
     9001 aaagcacccc gaggttgagc gacatgccaa cagagataaa gaacaaaccg agcagcaagc
     9061 ctttgaaggg atcgatagcc gtttccagtt catgacgata ttcactttcc gccagcagca
     9121 cgcccgcaat aaacgtacca agcgccatcg acagccccag cgcatccata aacattgcgg
     9181 aacccaacac cagcagtagc gtcgcggcgg tgaacacttc ccgcacgcca gaagctgcaa
     9241 taaaacggaa taccggacgc agtaaatagc gtcccccaat cagcatgccg acaaacgcca
     9301 gcaccttcat gccgatcttc atccagtcga aatgttcgtc tgccgaaccc gccaacaacg
     9361 gcactaacgc cagtgccggg atcaccgcca gatcctgaaa cagcagtacc gagaaaccta
     9421 actggccgga ttcgctgcga ttcatccctt tctcacgcat caattgcaac gccattgcag
     9481 ttgaagacat cgcaaggcca atgccaccga ccaccgccgc ctgccaggcg aaatccgtca
     9541 gcatcaataa tcccgccagc aacgccgcgc ttaacagcac ctgtgccgcg cctacgccaa
     9601 aaatcgaacg ccgcagttgc caaagcttgg agggattcaa ctcaaggccg atgataaaca
     9661 tcaggaatac cacgccgagt tccgaaaagt ggaggatctc atcgacgtcg ctaataaacc
     9721 ccagccccca cgggccaatc gcaatccccg ctagcaaata tcccaacact gcaccaatac
     9781 ccagccgcga agagaaacag cactcctgcg agtaaaaaat cagaaccttc catcagcggc
     9841 ctcctggaga cagcggattt gccagccagt caccgtaggc tctggcgtgg ctcgccagct
     9901 cctgtgcgct ttgccgtctc gcccagtaaa taatgatggg gcttaaccaa tgcatccggc
     9961 acatgcccgc cgccagttca aaggggcgca gaacatcgct catcggatag cgattcaacg
    10021 cgtcataacg gtaagcactt tccggctccc cagtggtaat cacgctacgc cagtactttc
    10081 ccgccagttg gtttcccccc ggtccgctgg caaaaccacg gcttaatacc cggtccagcc
    10141 actctttcag tagcgccggg cagctatagg tataaagagg atgctgaaag acaatcacct
    10201 cgtgctcgcg cagtaatgcc tgctcacgag ggatatcaat aaaaaagtcg ggatagtgcg
    10261 cgtaaaggtc gtgcacggta acattgctga gctgcgtggc cggtttaagc agaacccggt
    10321 ttgccaccga gtcctgagat tccggatggg catacagcag caaaactttc gctggctgag
    10381 acatcatccc cctcccggta tggtttttgt gtatagtcgc tgttttgggc taccattgcg
    10441 cccggtgcgg cagctcgccc atacattaca ttatcataat gataagttaa catagtctga
    10501 acatacggcg ccttatgatt gttttttcct cgttacaaat tcgtcgcggc gtgcgcgtcc
    10561 tgctggataa tgccaccgcc accatcaacc ctgggcagaa agtcggcctg gtgggtaaaa
    10621 acggctgtgg taaatctacc ctgctggcat tgctgaaaaa tgaaatcagc gccgacggcg
    10681 gcagctacac ctttccggga agctggcaac tggcgtgggt gaatcaggaa acgccggcgt
    10741 taccgcaagc ggcgctggaa tatgtcattg acggcgaccg tgaatatcgt caactggaag
    10801 cgcagctaca cgacgccaac gaacgtaacg acgggcacgc catcgccact attcatggca
    10861 agctggatgc tattgacgca tggagtattc gctcccgtgc cgccagcctg ctgcacggcc
    10921 tcggtttcag caatgaacaa ctggagcgcc cggtaagtga tttctctggt ggctggcgta
    10981 tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga cttgctgctg ctcgacgaac
    11041 cgactaacca cctcgatctc gatgccgtta tctggctgga aaaatggctg aagagctatc
    11101 agggcacgct gatcctgatc tctcacgacc gcgacttcct cgatccgatc gttgataaaa
    11161 ttattcatat cgaacaacaa agcatgttcg agtacaccgg caactacagt tcgtttgaag
    11221 ttcagcgcgc cacccgtctg gcgcagcagc aagcgatgta cgaaagccag caggaacgcg
    11281 tggcgcatct gcaaagttat atcgaccgtt tccgtgccaa agccaccaaa gcgaagcagg
    11341 cccagagccg cattaagatg ctcgagcgta tggagctgat tgccccggcg cacgttgaca
    11401 acccgtttcg ttttagcttc cgcgcgccgg aaagcctgcc taatccgtta ttaaagatgg
    11461 aaaaagtcag cgcaggctat ggtgatcgca ttattctcga ctcgattaaa ctgaatctgg
    11521 tccccggctc gcgcattggt ctgctaggcc gcaacggcgc gggtaaatcg acattaatca
    11581 aactgttagc cggtgaactt gcgccagtca gcggtgaaat tggtctggcg aaagggatca
    11641 agctcggcta cttcgcccag catcaactgg aatacctgcg cgccgacgaa tcgcctattc
    11701 aacatctggc acgtttagcg ccgcaggagc tggagcaaaa actgcgtgac tacctcggcg
    11761 gctttggttt ccagggcgat aaagtaaccg aagaaacgcg ccgcttttcc ggtggggaaa
    11821 aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc gaatctgctg ctgctcgacg
    11881 aaccgactaa ccaccttgac ctcgacatgc gtcaggcact gactgaagca ttaatcgagt
    11941 tcgaaggcgc gctggttgtc gtttcgcacg accgtcattt gctgcgttcc accactgacg
    12001 atctctacct ggttcacgat cgtaaagtcg aaccgttcga cggcgatctg gaagattatc
    12061 aacagtggtt gagcgacgta caaaagcagg aaaaccagac cgacgaagcg ccaaaagaga
    12121 acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg ggaagcggag ctgcgtgcgc
    12181 aaacccagcc actgcgtaaa gagattgccc gtctggaaaa agagatggag aagctgaacg
    12241 cgcaactggc gcaggcggaa gagaaactcg gcgacagcga actgtacgat caaaaccgta
    12301 aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc caaatccggc ctggaagagt
    12361 gcgaaatggc atggctggaa gcccaggagc agcttgagca gatgctgctg gaaggccaaa
    12421 gcaactgatg gcgcagataa caacgaccga tgccaatgaa tttagcagca gtgctaaatt
    12481 cacccccatg cgcggcttta gcaattgtca tctacaaacc atgctgccgc gtctgttccg
    12541 tcgcaaggtg aaattcaccc cgtactggca gcggctggag ttgcccgacg gcgattttgt
    12601 cgatctcgcg tggagtgaag atcctgcaca ggcgcggcat aaaccgcgtt tagtggtatt
    12661 tcacgggctg gaaggcagtc tcaatagccc ttacgctcac ggtctggttg aggcggcgca
    12721 aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga tgcagcggtg aaccaaaccg
    12781 tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc agttggtttt tacgctggct
    12841 gcaacgcgag tttggtcatg cgccaacggc tgccgtcggc tattcgctcg gcggtaatat
    12901 gctggcctgt ttgctggcaa aagaaggcaa tgatctcccg attgatgcgg cagtgattgt
    12961 ctctgcgccg tttatgctgg aggcctgtag ttaccatatg gaaaagggct tttcccgcgt
    13021 ttatcagcgt tacttgctga acctgttaaa agccaatgcc gcgcgcaagc tggcagccta
    13081 ccccggcaca ctgccgatta atctcgcgca gttaaaatcg gtacgtcgca tccgtgaatt
    13141 tgacgatctc atcaccgcca gaattcacgg ctacgctgac gctatcgact attatcgtca
    13201 gtgtagcgcc atgccgatgc tcaatcggat agccaaaccg acactgatta ttcacgccaa
    13261 agacgatccg tttatggatc atcaggtgat cccgaaaccg gaaagtctcc ccccgcaggt
    13321 ggagtatcag ttgactgaac atggcggtca tgttggcttt attggcggta cattacttca
    13381 tccgcaaatg tggctggagt cacgcattcc tgactggtta acaacgtatc tggaggcgaa
    13441 atcatgttga ttccgtggca agacctctcc cccgaaacgc tggaaaattt aattgaaagc
    13501 tttgtgttac gtgaaggcac cgattatggt gaacatgagc gtacacttga acagaaagtc
    13561 gccgacgtca aacgccagct acagtgcgga gaagcggtgc tggtatggtc ggagctgcac
    13621 gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat aacctgcaac cgtcggcgga
    13681 cgagataata atgatagcta agtcatggag ttactatgtc tgccaaacat ccggtcattg
    13741 cggtaacagg atccagcggc gcggggacca ccaccaccag cctcgcgttt cgtaaaatat
    13801 tcgcgcagtt aaatctgcat gcagccgagg tggaaggcga cagttttcac cgttataccc
    13861 gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc cgggcggcat atcagctact
    13921 tcggccccga agcgaacgac ttcggccttc tggaacaaac cttcattgaa tacggtcaga
    13981 gcggcaaagg gaaatctcgc aaatatctgc atacctacga tgaagccgta ccgtggaatc
    14041 aggtaccggg gacattcacc ccctggcaat ctttaccgga acccactgat gtactgtttt
    14101 atgaaggttt acacggcggc gtagtcacgc cacagcataa cgttgcgcag catgtggact
    14161 tactggtcgg cgtggtgcct atcgttaacc ttgagtggat tcaaaaactg atccgtgaca
    14221 ccagcgagcg cgggcactca cgagaagcag tgatggactc ggtagtgcgt tcaatggaag
    14281 actatatcaa ctacatcaca ccgcagtttt cccgcaccca tcttaacttc cagcgcgttc
    14341 ccaccgtcga cacctcaaac ccgttcgcgg caaaaggtat cccgtcgctg gatgaaagct
    14401 ttgtggtgat ccattttcgt aatctggaag ggatcgattt cccctggctg ctggcgatgt
    14461 tgcaaggctc attcatttcc cacatcaata cgttagtggt gcctggcggc aaaatgggtc
    14521 tggcaatgga attaattatg ctgccgctgg tgcaacgatt gatggaagga aagaaaatcg
    14581 agtaacgctg ctattacgcc ggataaaata ctatccggct tcacaacggg atagttaaat
    14641 cacgcggcaa ccacttcata cgagtgagta atattcaccg ctttttccag catcagcgcc
    14701 actgaacaat atttctcagc agagagatca accgcacgcg caaccgctgc gtctttcaga
    14761 tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa acaggcgtgg tgcctcttcg
    14821 cgacgctcag aggtcaattt tacttcacaa tcgaccacat cctgacgccc tttttgcagg
    14881 atagaaacca catcgatggc actgcaacca cccgctgcca tcaacaccat ttccatcggg
    14941 cttggtgctt tatcgcctga gttgccgtcc attaaaatct ggtgaccaga ggcggattcg
    15001 cccagaaaag ttaacccttc gacccacttc actcgcgctt gcatttttgc tactccactg
    15061 cgtcaatttt cctgacagag tacgcgtact aaccaaatcg cgcaacggaa ggcgacctgg
    15121 gtcatgctga agcgagacac caggagacac aaagcgaaag ctatgctaaa acagtcagga
    15181 tgctacagta atatattgat gtactgcatg tatgcaaagg acgccacatt accgtgcagt
    15241 acagttgata gccccttccc aggtagcggg aagcatattt cggcaatcca gagacagcgg
    15301 cgttatctgg ctctggagaa agcttataac agaggataac cgcgcatggt gcttggcaaa
    15361 ccgcaaacag acccgactct cgaatggttc ttgtctcatt gccacattca taagtaccca
    15421 tccaagagca cgcttattca ccagggtgaa aaagcggaaa cgctgtacta catcgttaaa
    15481 ggctctgtgg cagtgctgat caaagacgaa gagggtaaag aaatgatcct ctcctatctg
    15541 aatcagggtg attttattgg cgaactgggc ctgtttgaag agggccagga acgtagcgca
    15601 tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt cgtacaaaaa atttcgccaa
    15661 ttgattcagg taaacccgga cattctgatg cgtctgtctg cacagatggc gcgtcgtctg
    15721 caagtcactt cagagaaagt gggcaacctg gcgttcctcg acgtgacggg ccgcattgca
    15781 cagactctgc tgaatctggc aaaacaaccg gatgctatga ctcacccgga cggtatgcaa
    15841 atcaaaatta cccgtcagga aatcggtcag attgtcggct gttctcgtga aaccgtggga
    15901 cgcattctga agatgctgga agatcagaac ctgatctccg cacacggtaa aaccatcgtc
    15961 gtttacggca ctcgttaatc ccgtcagagt ggcgcgttac ctggtagcgc gccattttgt
    16021 ttcccccgat gtggcgcaga ctgatttatc accccgatat caactatgca cttcgacaaa
    16081 cgctggtgct atgtttgccc gtggtcgttg ggttaatgct tggcgaatta cgattcggtc
    16141 tgctcttctc cctcgttcct gcctgttgca atattgcggg ccttgatacg cctcataaac
    16201 gttttttcaa acgcttaatc attggtgcgt cgctgtttgc cacctgtagc ttgctgacac
    16261 aggtgctact ggcaaaagat gttcccctgc cctttttgct gaccggatta acgctggtac
    16321 ttggcgtcac tgctgagctg gggccattgc acgcaaaatt gcttcccgca tcgctgctcg
    16381 ccgccatttt taccctcagt ctggcgggat acatgccggt ctgggaaccg ttgctcatct
    16441 atgcgctggg cactttgtgg tacggattgt ttaactggtt ttggttctgg atctggcgcg
    16501 aacaaccgct acgcgagtca ctaagtctgc tgtaccgtga actggcagat tactgtgaag
    16561 ccaaatacag cctgcttacc cagcacaccg accctgaaaa agcgctgccg ccgctgctgg
    16621 tgcgccagca aaaagcggtc gatttaatta cccagtgcta tcagcaaatg catatgcttt
    16681 ccgcgcaaaa taataccgat tacaagcgga tgctgcgtat tttccaggag gcgctggatt
    16741 tgcaggaaca tatttcggtc agtttgcatc agccggaaga ggtgcaaaag ctggtcgagc
    16801 gtagccatgc ggaagaagtt atccgctgga atgcgcaaac cgtcgccact cgcctgcgcg
    16861 tgctggctga tgacattctt taccatcgcc tgccaacgcg ttttacgatg gaaaagcaaa
    16921 ttggcgcact ggaaaaaatc gcccgccaac acccggataa tccggttggg caattctgct
    16981 actggcattt cagccgcatt gctcgtgtgc tgcgcaccca aaaaccgctc tatgcccgtg
    17041 acttactggc cgataaacag cggcgaatgc cattacttcc ggcgctgaaa agttatctgt
    17101 cactaaagtc tccagcgcta cgcaatgccg gacgactcag tgtgatgtta agcgttgcca
    17161 gcctgatggg caccgcgctt catctgccga agtcgtactg gatcctgatg acagtattgc
    17221 tggtgacgca aaatggctat ggcgcaaccc gtctgaggat tgtaaatcgc tccgtgggaa
    17281 ccgtggtcgg gttaatcatt gcaggcgtga cgctacactt taaaattccc gaaggttaca
    17341 ccctgacctt gatgctgatt accaccctcg ccagctacct gatattgcgc aaaaactacg
    17401 gctgggcgac ggtcggtttt actattaccg cagtgtatac cctgcaactg ttgtggctga
    17461 acggtgagca gtacatcctt ccgcgtctta tcgataccat tattggttgt ttaattgctt
    17521 tcggcggtac tgtctggctg tggccgcagt ggcagagcgg gttattgcgt aagaacgccc
    17581 atgacgcttt agaagcctat caggacgcga ttcgcttgat tctgagcgag gacccgcaac
    17641 ctacgccact ggcctggcag cgaatgcggg taaatcaggc gcataacact ctgtataact
    17701 cattgaatca ggcgatgcag gaaccggcgt ttaacagcca ttatctggca gatatgaaac
    17761 tgtgggtaac gcacagccag tttattgttg agcatattaa tgccatgacc acgctggcgc
    17821 gggaacaccg ggcattgcca ccggaactgg cacaagagta tttacagtct tgtgaaatcg
    17881 ccattcagcg ttgtcagcag cgactggagt atgacgaacc gggtagttct ggcgatgcca
    17941 atatcatgga tgcgccggag atgcagccgc acgaaggcgc ggcaggtacg ctggagcagc
    18001 atttacaacg ggttattggt catctgaaca ccatgcacac catttcgtcg atggcatggc
    18061 ggcagcgacc gcatcacggg atttggctga gtcgcaagtt gcgggattcg aaggcgtaat
    18121 acaggctaaa ccatcggatg aggtgttcac gccgtatccg acatcttttg cctgatgcgt
    18181 cttatttacg ccccaaccac cttcgccacc gcgtgggcga aacgatgcat cccttcatcg
    18241 atatccgcat cttccaccac cagcgacggc gcaaaacgca tcacatcagg cccggcattc
    18301 agcaccattg cgccttcctc tgcacccgca tacaggaaat cacgcgcccg acctttgtac
    18361 tgtggtttca gctctgcgcc aatcaacagc cccataccgc gaatatcgct aaacacatcg
    18421 tactgctgat cgatcttctg caaatggtca acaaaacgct ggcgtttcgc ctgaatacct
    18481 tccagcactt caggggtatt gatgatatca aacgctgcgc ccgctactgc acaggccaga
    18541 ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa acgcggaagc aatctccgcc
    18601 gtggtcagca tggcgctaac cgggaagccg ccacctagcg ctttcgcaga ggtcagaata
    18661 tccggcgtca cgccgtagtg catataagca aacagatcgc cggtccgccc catcccgcac
    18721 tgcacttcat caaacaccaa taatgcctga tgttgatcgc acagctcgcg caagccctgc
    18781 aaaaactctg gcgtcgctgc cgtcacgccg ccctcgccct gaatcggctc aaccaccacc
    18841 gcacaggtat ggtcatccat cacggctttt actgcgtgaa gatcgttaaa aggcacgtgg
    18901 atgatgtctg ccggttttgg cccaaagccg tcggaatatt ttggctgccc gcccaccgag
    18961 acggtaaaca gcgaacgacc atgaaaagcg ttatggaagg caataatttt ggttttgaac
    19021 gggctatgac gcacacaggc gtaatggcgt gccagtttaa aggcggtttc gttagcttcc
    19081 gtgccggagt tcataaacac cacgcgttcg gcaaacgttg cctcaatcag tttacggcca
    19141 agacgcagcg ccggctcatt ggtgaaaacg ttactaatat gccacagagt ttcgccctgg
    19201 gtttttaacg cgttcaccag cgcaggatgg caatggccca acgccgtaac tgcaatgcca
    19261 cccgcgaaat cgacatactc cttgccctgt tgatcccaga ttcggctgcc ctgacctttt
    19321 accggaataa actctgccgg agcataaatc ggcagaatca cttcatcgaa agtcgcgcgt
    19381 gtaattgctg tttgttcaat tgccatctca tgatcaccct gttacgcata aataaatatg
    19441 aaattataat cacaaaatat gcataaaaaa tcactgaatg gcaatcagaa atcagcgatg
    19501 cagaaaatta gccagcagtt gatgtccttg ttcgctaaga atactttctg gatggaattg
    19561 cacaccttcc agatcccact ggcgatggcg aatccccata atctcgcggg tttcgctcca
    19621 ggccgtcact tcaaagcact ccggtaatga gtcaggttcc accaccagcg aatggtagcg
    19681 tgtcacggtg agtggatttg ccagcccctt aaatacgccc acaccattat gtgtaatcgg
    19741 cgaggttttg ccgtgcatga cctttgcggc gcgcacaact ttgccgccaa atgcctgcgc
    19801 cattgcctga tgaccgaggc agacgccaag aatcggcaag cgtccggcat agtgacgaat
    19861 aacgtcaagg gagatcccgg cttcatctgg cgtacagggg ccgggtgaga tgacaatttt
    19921 ttgtggttta agggcgtcga tatccgccaa cgtcaacgca tcgttgcgct taaccaacac
    19981 atccgccccc agttcacaaa agtactggta gaggttccag gtaaaagaat cgtagttatc
    20041 aataagcagg atcatggcgg ctccggtacg aaagaaccgc gctattttac tcagattccc
    20101 cggcttcgct taccactttg ctaaatatca tctgcagcgc ggtgagatcc cccattgccc
    20161 cattctgatt tgcctgattc caggcctctt tttcgatacc ctgccagctc agttgatatc
    20221 cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc caaaccactt cccacccgga
    20281 agggatgcag cacgttgatt tcacaatagt aatgcgccag ccgctcgacg aacttcgctt
    20341 tctccaggcc aaccagataa ccttcttcct ccagatcctg catcagggca ttgccctctt
    20401 tttcgatata agcaaagtgg cagaacggcg tatcgccctg ataaatatca acttcacgca
    20461 gttgccccgc ccagtcgaaa atatcctgat acagctggcg atggatagtg cgcaaatgcg
    20521 gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc acgcagcgcc gtcatttcgt
    20581 aagcgacctg ttccagccgc tgctgctggc ggatgttcaa ccggttacgc atgatatcaa
    20641 ggcctggata aagatacgga tcgcgccctt cgccgaattt atcgctcata gtgcctcctc
    20701 agatcttcaa gacgcgctaa agcctctgcg gcagtaagag tgactaaagg catctcgacg
    20761 ccttcaagac ggcgactggc ctggaaatta cgattacgct gaagctccca gagacgggac
    20821 ttttgcttat cggtgagttt cttcacttga tgcctccctg aacgtgtcca tttgccacaa
    20881 gtataagcgg caaatccggg ttacgccggg agagcagaag cgcgggcaag attgcccgcg
    20941 cgagaaatca ttacggcagg actttagcgg aaaggataac taccggtttt gacggcacat
    21001 tctggtacgg accaacgtca tgagtcggca cctgggaaat cttatcggca acgtccatgc
    21061 ctttcaccac tttaccaaat accgcgtaac cgaaatcacg ctgaccgtgg tcaaggaagg
    21121 cgttatcggc aacgttgata aagaactggc tggtggcgct gtctttgtca gcggtacgcg
    21181 ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc agcttcattt ttgattggcg
    21241 ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc gccctgaatc ataaagccag
    21301 gaatgacgcg gtgaaaggtc gtgttgttat aaaagccact gttcacataa tcgacaaagt
    21361 tttgcacaga cactggcgct ttttgtttat ccagctccag ttcgatgtta ccagctgagg
    21421 ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc tgcgggagaa agagcagaaa
    21481 gagcgaaaac agcagccatc gccgccaggg tcgatttgaa catgagattt ccttaacaaa
    21541 cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc ttcaagaaca aggccatccc
    21601 tttacctaat tttacgtatc tgaaatatat gtaacctgag gataactttt tattccgtga
    21661 tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc aataaaacat ttaatcagat
    21721 cacaaaatcg cctaaaatcg cccgctcgcg gcatagcccg ctatgccatt ttatttttga
    21781 acgcaggcca atcatgacta acagcaatcg catcaagctc acatggatta gctttctttc
    21841 ctacgcactg accggtgcgt tggttattgt caccgggatg gtgatgggaa atatcgccga
    21901 ttatttcaat ctgcctgttt ccagcatgag taacaccttc accttcctca atgccggcat
    21961 tttaatctct atcttcctca acgcctggct gatggaaatc gtcccgttga aaacgcagtt
    22021 acgttttggc tttctcctga tggtgctggc ggttgccggt ttgatgttca gccacagcct
    22081 ggcgctgttc tcggcggcga tgttcattct cggggtggtc agcggcatca ccatgtcgat
    22141 tggtacattc ctggtaacac aaatgtatga aggacgtcag cgcggttcac gcctgttatt
    22201 taccgactcc ttcttcagta tggccgggat gattttccca atgatcgccg cgtttctgct
    22261 ggcacgtagc attgagtggt actgggttta tgcctgcatc gggctggtgt atgttgctat
    22321 ttttattctg accttcggct gtgaattccc ggcactgggc aaacatgcgc caaaaacaga
    22381 tgctccggta gagaaagaaa agtgggggat cggcgtactg tttctctctg ttgcggcgct
    22441 gtgctacatc ctcggtcagt taggttttat ctcctgggtg ccagaatatg ccaaaggtct
    22501 gggtatgagc ctgaatgacg ctggcacgct ggtaagtaac ttctggatgt cgtacatggt
    22561 cggcatgtgg gcgttcagct ttattcttcg cttctttgat ttgcagcgca ttctgaccgt
    22621 actggctggt ctggctgcga ttctgatgta cgtctttaac accggaacac cggcacatat
    22681 ggcgtggtca attctcgccc tgggcttctt ctccagcgcg atctatacca ccatcatcac
    22741 cctgggttca cagcagacca aagtaccgtc gccaaaactg gttaactttg tcctgacctg
    22801 cgggaccatc ggtactatgt tgacctttgt ggttaccggc ccgattgttg aacatagtgg
    22861 tccgcaggcg gcgctgctga cggcaaacgg tctgtacgct gtcgtctttg tgatgtgctt
    22921 cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc attaattgtt
    22981 catgccgggc ggcactatcg tcgtccggcc ttttcctctc ttactctgct acgtacatct
    23041 atttctataa atccgttcaa tttgtctgtt ttttgcacaa acatgaaatg tcagacaatt
    23101 ccgtgactta agaaaattta tacaaatcag taatataccc cttaaggagt atataaaggt
    23161 gaatttgatt tacatcaata agcggggttg ctgaatcgtt aaggtaggcg gtaatagaaa
    23221 agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg gtatggtcgg
    23281 ccatcgcttt atcgaagatc ttcttgataa atctgatgcg gccaactttg atattaccgt
    23341 tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac ctctcgtctt acttctctca
    23401 ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac acggcatcaa
    23461 agttctggtc ggcgaacgcg ctatcaccat caaccgtcag gagaaggtga ttcactccag
    23521 cgccggacgt accgtttttt atgacaagct gatcatggcg accggttcct acccgtggat
    23581 cccgccaatt aaaggttctg atactcagga ctgctttgtc tatcgcacca ttgaagacct
    23641 caacgccatt gaatcctgcg ctcgtcgcag caagcgcggt gccgttgttg gtggcggcct
    23701 gttaggtctg gaagctgctg gcgcgctgaa aaacttaggc attgaaaccc acgttatcga
    23761 atttgcccct atgctgatgg cggaacagct tgatcagatg ggcggcgagc agctgcgtcg
    23821 caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa aacactcttg agattgtgca
    23881 ggaaggtgtt gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac tggaagtcga
    23941 ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc agtgtggtct
    24001 ggacgttgct ccgcgtgggg gtatcgtcat aaatgattcc tgccagactt ccgatccgga
    24061 tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc tggtagcacc
    24121 aggctacaaa atggcgcagg tcgccgttga ccatattctt ggtagcgaaa acgcctttga
    24181 aggtgcagac cttagcgcca agctgaaact gctgggcgta gacgtaggcg gtattggtga
    24241 tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac ctcgacgaaa gcaaagagat
    24301 ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg ctcggtgcgg tactggtggg
    24361 cgataccagc gactacggca acctgctgca actggtgctg aacgctatcg agctgccgga
    24421 aaacccggat tccctgatcc tgccagcgca ctcgggtagc ggtaagccgt ctatcggtgt
    24481 tgataaactg ccggacagcg cgcaaatctg ctcctgcttc gacgtcacca aaggcgacct
    24541 gattgctgct atcaacaaag gctgccacac cgttgcggcg ctgaaagccg aaaccaaagc
    24601 cggtacgggc tgcggcggtt gtattccgct ggtcactcag gtactgaacg cggaactggc
    24661 gaaacagggt atcgaagtta acaacaacct gtgtgaacac tttgcttatt cgcgtcagga
    24721 actgttccat ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc tggcgaaaca
    24781 cggcaaaggt tacggttgtg aagtttgtaa accgaccgtc ggttcgctgc tggcctcctg
    24841 ctggaacgaa tacattctga agccggaaca tactccgctg caggattcta acgacaactt
    24901 cctcgcgaac atccagaaag acggcaccta ctcggtgatc ccgcgttctc cgggcggtga
    24961 aatcaccccg gaagggctga tggcagtggg tcgtatcgcg cgtgaattta atctctacac
    25021 caaaatcacc ggctctcagc gcctggcgat gtttggcgca cagaaagacg atctgccaga
    25081 gatctggcgt caactgattg aagccggctt cgaaaccggt catgcctatg cgaaagcact
    25141 gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc tacggcgttg gcgacagcgt
    25201 cggcctcggc gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc acaaaatgaa
    25261 gttcggtgtc tccggctgta cccgtgaatg ttcagaagct cagagtaaag acgtgggtat
    25321 tatcgccacc gaaaaaggct ggaacctgta cgtttgcggt aacggcggca tgaaaccgcg
    25381 tcatgcggat ctgctggcag cggatatcga tcgcgaaacg ctgatcaaat atctcgaccg
    25441 cttcatgatg ttctacatcc gtaccgccga caaactgacg cgtaccgcgc cgtggttaga
    25501 aaacctcgaa ggcggcatcg attacctgaa agcggtgatc attgacgaca aactggggct
    25561 gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt gtgagtggac
    25621 tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa cacttcatca acagcgacaa
    25681 gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg caacgccgta
    25741 tgaacgaatc ccggtaactc tggtggagga caacgcatga gccagtggaa agacatctgc
    25801 aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc tgttaggtga cgaacaagta
    25861 gcaattttcc gcccgtatca cagcgatcag gtgtttgcga tcagcaacat cgacccgttc
    25921 ttcgagtcca gcgtgctgtc acgcgggttg attgcggaac accagggcga gctgtgggtc
    25981 gccagtccgc tgaaaaagca gcgttttcgc ttaagcgacg gcttgtgcat ggaagacgaa
    26041 cagttttccg tcaagcatta cgacgcgcga gtgaaagacg gcgtggtaca gctgcgcggt
    26101 taatatttta acgggaggcg caatgcctcc cctttttgca tggtcctgta ataaccttcg
    26161 gtatattgca ggacattttt taaacttttt gttttatttt ttgtttttat tttttaaagg
    26221 ataatcaaat gtttacagac actattacta agtgtgcggc taacgctgcg cgcattgcac
    26281 gcctgtcggc aaataacccg ctcggctttt gggtcagctc cgccatggcg ggcgcgtatg
    26341 tgggtcttgg gatcatcctg attttcacgc tcggtaattt gctcgacccg tccgtacgtc
    26401 ctctggtgat gggcgcgacc tttggtatcg ccttaacgct ggtcattatt gctggttctg
    26461 aactgttcac cggacacacc atgttcctca cctttggagt gaaagcgggc actatcagcc
    26521 acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg taacctggtt ggttccgtct
    26581 ttgttgccat gctctatagc tggggcggcg gtagcctgct gccggtagat accagcatcg
    26641 ttcactccgt cgcgctggct aaaaccactg caccggcaat ggtactgttc ttcaaaggtg
    26701 cattgtgtaa ctggctggtt tgcctggcta tctggatggc gctgcgcact gaaggggcgg
    26761 cgaaatttat cgctatctgg tggtgtctgc tggcatttat cgcgtccggc tacgagcact
    26821 ccatcgctaa catgacgctg ttcgccctct cctggtttgg caaccacagc gaagcctaca
    26881 cgctggcggg tattggtcat aacctgctgt gggtgacgct gggtaatact ttatcaggtg
    26941 ccgtattcat gggattgggt tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg
    27001 acaaatttaa tcaaactgaa acagctgccg gttaattact aaggggtttt tacgtggatc
    27061 atttgcctat attttgccaa ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg
    27121 tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat
    27181 tagcgtttat tccacagttc accgcatggg cagatgcagg catgttaacc ctcgtcgaag
    27241 ggccatttga tgaaagcctt ctcgacacct gctggctggc gattgcagcg acagatgatg
    27301 acgcgcttaa ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtgg
    27361 tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat tattgaccgc tcgccgctca
    27421 tggtagcggt ctcctctggc ggcacctctc cggttctggc gcgcctgttg cgcgaaaaac
    27481 tcgaatcact gctgccgtta catctgggcc aggtagcgaa atacgccggg caattacgcg
    27541 ggcgagtgaa acaacagttc gccacgatgg gtgagcgtcg ccgtttctgg gagaaattgt
    27601 tcgttaacga ccgcctggcg cagtcgctgg ccaacaacga tcagaaagcc attactgaaa
    27661 ccaccgaaca attaatcaac gaaccgctcg accatcgcgg tgaagtggtg ctggttggcg
    27721 caggtccggg cgatgccggg ctgctgacac taaaaggact gcaacaaatt cagcaggcag
    27781 atgtggtggt ctacgaccgt ctggtttctg acgatattat gaatctggta cgccgcgatg
    27841 ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg cgtaccgcag gaagagatta
    27901 accagatcct gctgcgggaa gcgcaaaaag gtaaacgcgt ggtgcgtctg aaaggcggcg
    27961 atccgtttat tttcgggcgt ggcggagaag agctggaaac actgtgcaac gcgggtattc
    28021 cattctcggt ggttccgggt attaccgcag cttctggttg ctctgcctat tcgggtattc
    28081 cgctcacgca tcgcgattat gcccagagcg tacgcttaat tactggacac ttaaaaaccg
    28141 gtggcgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg ttttatatgg
    28201 ggctgaatca ggctgcaact attcagcaaa agctgattga atacggtatg cctggtgaaa
    28261 tgccggtggc aattgtcgaa aacggaacgg cagtcacgca gcgcgtgatt gacggtacgc
    28321 tcacgcagct gggcgaactg gcgcagcaaa tgaacagtcc atcgctaatt attattggtc
    28381 gggttgttgg cctgcgcgat aaattgaact ggttctctaa ccattaattt aaccagggcc
    28441 agagaagtct ggcctttttc gtttacaact ctctttatat attcagcaga taaaaacata
    28501 accccttaat aaatattgtc tgctaatggg ttattttcac cgttacagcg acttacaaaa
    28561 ttaaatcaat cgctcactga cgctaattga atattcctgt atcgttctta atgagcagtc
    28621 tgcttatatt catataatca atgaatgtta attaatatat aatacatagg gaatgtaaat
    28681 gaacaaattt attaaagttg cactggtagg cgcagtactg gctacgttaa ctgcatgtac
    28741 tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc acccggcaat
    28801 ttctatttct aaaatcattg gcggttgcgg tccgactgca cagtaatcca ttgacagaaa
    28861 tcggctaacg ccggtttaat tgcctgacgc cctcggagtt atccgagggc gtatttgttt
    28921 ctgcattctt ttcaaaaata tcgtttacaa cgattaaatg ctggcaatac tttcacgcaa
    28981 gatccgcaat atcgtctctt caccgctgcg atttgggtta atgcgaatcg cgcaatgttc
    29041 taactgcgga ttcgcctggc gaaacgttcc ggaaagtcga taaaagagcg gcgggatttc
    29101 atattttgac tctgcaccaa ccgggtaagg caaggcaccg agtttctgcg cctcttccag
    29161 tactctggcg gcaatcggct gatgaaactc cacaatcaac accttcgact gcgcattggc
    29221 aatcaccgcg cttttcactt ccgccactgc gccaccgtta agtaaagcca gcaaccgttc
    29281 agatactccc gcctgcaccg catgtatcac cggcgcaaac accagcccac gcaagacttc
    29341 cagcgcctgt gcaccctgaa tctggctgcc gccggaatag agcgtggcgc gaatccgacc
    29401 aataacatcg gcatcgccta ccaccgcacc aacgccctct ggcccaaaca gcttgaagca
    29461 ggaaaatgtc gacacattcg ccccgcattc gcagccgatt cgcgctacct tcatcaccgc
    29521 atagttgtca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca gcacgtctgc
    29581 cagcacatag cggtcctgcg gctgctggcg cgtatgctgc accagcgctg catcaggttg
    29641 ttgctcgtca acaacctgct tcagtgccga cagatcattg aaatcagccg taatgagcgt
    29701 tagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt catgcaccag
    29761 aagacgctgc cccgatttga gcaaagccgc cagtgccgca cggatcgcgc cagtacccgc
    29821 gccctgtacc agcgccgcag cctgtgcgtg aaatgcatca gccagcacct gttccacacg
    29881 ctgggtaatg cggggctgat ttagccccgg cgttaagccc aaatcaccgc aggtaagaaa
    29941 ctcgctgcca ggaaaatgac ggcaaatcgt gtccaccagc gcaaactgct tttgctgcgc
    30001 ctcaataagc gtcaggcttt gcagaggaaa cgtcttcata ggtgccttcc ttaagccgga
    30061 acgaacaggc caagccagta aagaacattc agcaggatgc cggtgatcat tactgctacg
    30121 accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa gtaaatggca
    30181 accgcgatag agaatccggt atagcccgcc attttgattg ccgcaaagat cgaaccgacc
    30241 agcaacgcca cttccatcag catattcatg gcgttacgga tgttatccga cgcattacgt
    30301 accgacgggt agcgccccaa ccatttgccg atcgaacgaa gcagcaagac ttccgccgaa
    30361 ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata gcccaccgca
    30421 taaacaaagg taaagcccgc aactgcatac acaccggtgg ctaacgcggt ggtggcaatc
    30481 aacggcacaa aacccagccc gcgcataaat tctgccagtg ccgcctggtt gattagtgtt
    30541 tgcgattgtt ccggcgttac gcctgcggag tacgctttct ccagtgtgaa gatcgacact
    30601 tcactaccgg cgaaaatctt catgctggca acggcagcaa tcaatgcacc cacgatggcg
    30661 atatacggta agtttttgat aatccgtgat gtacgctctt caaacaccga cataccgctg
    30721 gcatcgatat cattttcatc acgatggcgc aggtcatgag ttattgcgat ccccagcagc
    30781 atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa atagcgcact
    30841 accaccacac gagtcatcag taccaccacg gcggcgatca gactttgctt ccagccaaac
    30901 tggtagaaaa tcgccaccaa cgggaacagt gcaaaagctg aaaccaccgg cgagcttaat
    30961 tcccccaggc tacctaatac atccaccggt agcgcggtca gcagctggtt tactggcaac
    31021 agacaggtga ggatcaatat tccccagata gcaccaagac caaacgccat caggctgttt
    31081 atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt gagcagcccg
    31141 gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag accaaaagcg
    31201 atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag gatcgggcgg
    31261 ataccgtcat gaaaaacggc tgccgagcga tgcgccagaa gcgatgtcat acccgtcagg
    31321 cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat ttcagatggt
    31381 taatcagcat cggaatcgcg tgttcaacgt gctcaacgga aaggccaaac gccacttttc
    31441 cttcagcgat cattttggcg atatgttcgt ctttcgcttt aatgcccggc ttggcaatgg
    31501 tgcaactttt gttatagcca atcaccgcga tagcaatcga caaggccgcg cccgcaccgg
    31561 tattacacgc gccgatgtag taatccagtt gcccggattt cactttcatt gccgcttcca
    31621 tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg gtttttttaa
    31681 tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc ttattggaaa
    31741 aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa tcctgactgg
    31801 cgcaattgcg gaataaaagt ggtcaataaa aagtcatagc cataaccacc gttggctttt
    31861 aaatgagagc gccgcgtaat atccatcgac agcatgacgc gattcagcac cccacggtca
    31921 cgtagcgcat ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt cttgccgatg
    31981 gtgtcgaact gcacatacgc gccgagatcg atcatcttca aaatgttgtc gagattgtct
    32041 ttcagatcgc agtggccgac ggtgacgcgc gaaagatcaa ctccgtgggc ttgcaacaga
    32101 gccagttgct ccagccccat cgtgctgaac gacgtatgcg tggagatcgg gcgtccggtt
    32161 tggttatgtg ccagcgcggc ggcaataaac accttctctt ccagcgacgt aatcttccct
    32221 tcgctggagc cgatctccgc gataattccg gctttcaggt cagtgccatc gataccctgc
    32281 tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcgc cacatgttcc
    32341 gggaaaaacg cgtcctggta ataaccggta caggccacca cgttgatccc ggtttcgcgc
    32401 attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat ctcaatcaca
    32461 ttacgcacgc cccgggccat caggtcgttc atctcctggc aaatgaacgc atactgatca
    32521 aggcggcagt ccacgttgtt tttaaagccg gaaagatcaa tatgcagatg ctcatgggcg
    32581 agggtgtagc ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg aaggaggaaa
    32641 gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg gtagccccca
    32701 catcagaaag cgtggtgcgc acaccgagct gcgtagcgac cagtccttgc tgataaacca
    32761 gcactggcac cacttcgcgg gtatggtggc tgtgaccaat ggtcggatcg ttaccgtgat
    32821 ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg gcaaggttac
    32881 ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga ccagcgaggt
    32941 cggtttcctg gatattggta caaataaacg ccgtcggata ggtgttaaat tcgttgaggg
    33001 tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacgcca taaggattgc
    33061 tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca tacagttttt
    33121 gcggtacctg cactttttca tcgacaccgt agcccatatg tacgacctgg aaaccgttgt
    33181 cataggcgcc agaacgcggc gcattgatgc caataaagcg cccttctttg ctttctgcgg
    33241 catcgaggat gcactggctg tcggttaaca ggccaccaaa tgtaatgacc cgaccgacct
    33301 gtacctgctc acgcacgata cgacccattt tgattgcgtc gtcaaaagag atcacagaga
    33361 gattggcggt gatgttatag acctgaccta aatccgcctc gagattatcg ccaatcgcaa
    33421 ccgcctgatt gacccacaga aattgcagat catcgccacg acgctcaacc tgccagccag
    33481 cggaaactaa tgcctgctca acacggtcaa tcacatcacg aaagggcatc cgcagcggcg
    33541 gtaatgggcg cgtacctaaa atctcctgat gccccataaa ggtatcgcca ccttcatgtt
    33601 gcagctctgc cacgccccag gttgcggagt ctgacggctg catatcgcca ggcgcataac
    33661 ccaatgcgtt gattagcccc agcgtctcca gcgttggtag ctgcaaatgc ggcaactggc
    33721 tcaggatgtg accacaggta ttcgccccgg catcctgcgg acgcaccaac gtgacatctt
    33781 tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc atttacgcct
    33841 ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgcca cggtgaatac
    33901 cggacaccag caccacatcg ctacgggtga taaaaatctg cgtgcgaaaa cagagcacca
    33961 ctgcactgct taccggatgc tcgcctgcca gcggcagggt gtagtcgata ctgctgtcat
    34021 caacggcgtt gagatatgtt tcagtaattc tttgattttc tggcgtaaac accagcgcat
    34081 gttgcgcgtg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg ccacggaaat
    34141 gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg ccctgttgat
    34201 ttgccggaat agtacccgtc agcgcatgac cgggttcggt atgagtcaca ccgtattccg
    34261 ccagcaatgg cagcgacgtg cagctggtcg ctgaaggcgc gttcagttgc tcaatcgcaa
    34321 taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc ggtgtcggca
    34381 aaactttccc ggcagcctca tcccaaagca ggcaagggaa atgggtaagt cccgccaaat
    34441 gtagccccgg cagattctgg atttcagcaa ccacctcgtg cagcgaatgc tgtacaaaac
    34501 cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc agcacagact
    34561 gcacacgtcc ggccttcacc gctgccgcag aaacttcccg cgctttgtcg agagtaaaca
    34621 cggtgatgac gtcggtgccc tgttcaacgg cgtcagaaac ctgatgacaa gggatttgta
    34681 ccagatgccc ctgatgcgct accggcaaac cggcgcggcg catgactcgc gcctctttgt
    34741 aatccaccgc cacaatgccg ctgtagccca gagccagcag tttttctgcc agccacggat
    34801 tacgaccgaa ttgtttggtc atcagataca gttcaatgcc gtaaagccgc gccgtctcaa
    34861 tcagccgctt gccgttttcc agaacttgat ccacgtcgat cacccaactg tccggggcga
    34921 tcttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccggattc tggcgtttca
    34981 atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata ttcatttttt
    35041 tgaatattta ggttgtgcga cgtggattta tcgggagctt caaacctctt cgttagcagc
    35101 catccataat ccataaaggt tggccagcaa atagccttct tcacacgggt taacttccag
    35161 tgcaaattcc ttcaacaata cctgatgcaa ttgcacaacg gcttgccagt ggctggattg
    35221 cgccagttcc gccagcaatt cgtcatccag cggctctatt tcttcaccac ggcgactgcg
    35281 catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc gcaccggtag
    35341 atggcactct gtttccagta cattgacgac ctgcatcatg cctttgcaga tgtccttatc
    35401 aataacgcct gcatcgcaaa gcaggttgag tctggtttcc atatcagatg cctttcgttt
    35461 aataactggg ttcctgttcc ccactgacta ctcgctgttg atgagcaagc agggcatgtt
    35521 tatcaacaac tgcacgcagc agttgttgca ttgggtaatc atcggcacgc gtcaggatca
    35581 cggcttcggt ggcctgtaaa aatcgcggat cgtctgtgag cggcgtcgct tctaatccca
    35641 gcatagtcag ttcgttttcc gccactacgt tccagataac cgcgtcaaca tcgcctttaa
    35701 caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca ctatcgccaa
    35761 aaaagacatc ggtcatgatt ttctgatctg ccgaacggtt atccagcccc acgcgcttca
    35821 cgtttgcgga ctcgccttta cggcaaatca actggtgctc gccaacgtag gtatgcggtc
    35881 ccaactccag cgcgaggcat aatccttttt gcgtgagata actttccgcc gccagtcgcg
    35941 aaaccaccgc catgtcatac acgccattaa gtaaacactc cacgcgaata tccgcgccgc
    36001 gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg ctcgccaggc
    36061 cttcgtacaa acgggtatag ggcaagggca tcgcacatac cacgttgttg atatccacat
    36121 gagtcagcaa tgctttgtta tccatctcga ccagataact gccattgcgc ccacggcgtt
    36181 caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc aggccaaccg
    36241 atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg cacttttcac
    36301 ccaacaaata acgggccagt gtcgtgataa cgacgccttc tttttt
//
LOCUS       DAZKHK010000039        36294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKHK010000039 DAZKHK010000000
VERSION     DAZKHK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.039"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..230
                     /locus_tag="ACHOS5_001954"
     CDS             <1..230
                     /locus_tag="ACHOS5_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776772.1"
                     /translation="LDLFLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYH
                     SDNSGSGGGSSGGGFSGGGGSSGGGGASGRW"
     gene            complement(290..1588)
                     /gene="eno"
                     /locus_tag="ACHOS5_001955"
     CDS             complement(290..1588)
                     /gene="eno"
                     /locus_tag="ACHOS5_001955"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9776773.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1676..3313)
                     /gene="pyrG"
                     /locus_tag="ACHOS5_001956"
     CDS             complement(1676..3313)
                     /gene="pyrG"
                     /locus_tag="ACHOS5_001956"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP9776774.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARYARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3541..4332)
                     /gene="mazG"
                     /locus_tag="ACHOS5_001957"
     CDS             complement(3541..4332)
                     /gene="mazG"
                     /locus_tag="ACHOS5_001957"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP9776775.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4404..4739)
                     /gene="mazF"
                     /locus_tag="ACHOS5_001958"
     CDS             complement(4404..4739)
                     /gene="mazF"
                     /locus_tag="ACHOS5_001958"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP9776776.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4739..4987)
                     /gene="mazE"
                     /locus_tag="ACHOS5_001959"
     CDS             complement(4739..4987)
                     /gene="mazE"
                     /locus_tag="ACHOS5_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP9776777.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5065..7299)
                     /gene="relA"
                     /locus_tag="ACHOS5_001960"
     CDS             complement(5065..7299)
                     /gene="relA"
                     /locus_tag="ACHOS5_001960"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP9776778.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRDSVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7347..8648)
                     /gene="rlmD"
                     /locus_tag="ACHOS5_001961"
     CDS             complement(7347..8648)
                     /gene="rlmD"
                     /locus_tag="ACHOS5_001961"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9776779.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDCVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQLWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8705..11461
                     /gene="barA"
                     /locus_tag="ACHOS5_001962"
     CDS             8705..11461
                     /gene="barA"
                     /locus_tag="ACHOS5_001962"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP9776780.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11692..13032)
                     /gene="gudD"
                     /locus_tag="ACHOS5_001963"
     CDS             complement(11692..13032)
                     /gene="gudD"
                     /locus_tag="ACHOS5_001963"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP9776781.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13053..14393)
                     /locus_tag="ACHOS5_001964"
     CDS             complement(13053..14393)
                     /locus_tag="ACHOS5_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP9776782.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRTLKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14395..15747)
                     /gene="gudP"
                     /locus_tag="ACHOS5_001965"
     CDS             complement(14395..15747)
                     /gene="gudP"
                     /locus_tag="ACHOS5_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP9776783.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16182..16631)
                     /locus_tag="ACHOS5_001966"
     CDS             complement(16182..16631)
                     /locus_tag="ACHOS5_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9776784.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(16649..17431)
                     /gene="truC"
                     /locus_tag="ACHOS5_001967"
     CDS             complement(16649..17431)
                     /gene="truC"
                     /locus_tag="ACHOS5_001967"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP9776785.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17431..17760)
                     /locus_tag="ACHOS5_001968"
     CDS             complement(17431..17760)
                     /locus_tag="ACHOS5_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP9776786.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18382..18927)
                     /gene="syd"
                     /locus_tag="ACHOS5_001969"
     CDS             complement(18382..18927)
                     /gene="syd"
                     /locus_tag="ACHOS5_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP9776787.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18995..19843
                     /gene="queF"
                     /locus_tag="ACHOS5_001970"
     CDS             18995..19843
                     /gene="queF"
                     /locus_tag="ACHOS5_001970"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP9776788.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVCQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNNDFVPST
                     TRLVRQ"
     gene            19955..21319
                     /gene="ppnN"
                     /locus_tag="ACHOS5_001971"
     CDS             19955..21319
                     /gene="ppnN"
                     /locus_tag="ACHOS5_001971"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP9776789.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21876..23165
                     /gene="sdaC"
                     /locus_tag="ACHOS5_001972"
     CDS             21876..23165
                     /gene="sdaC"
                     /locus_tag="ACHOS5_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP9776790.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23223..24590
                     /gene="sdaB"
                     /locus_tag="ACHOS5_001973"
     CDS             23223..24590
                     /gene="sdaB"
                     /locus_tag="ACHOS5_001973"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP9776791.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24702..25457
                     /gene="xni"
                     /locus_tag="ACHOS5_001974"
     CDS             24702..25457
                     /gene="xni"
                     /locus_tag="ACHOS5_001974"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP9776792.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     EPHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25562..26710)
                     /gene="fucO"
                     /locus_tag="ACHOS5_001975"
     CDS             complement(25562..26710)
                     /gene="fucO"
                     /locus_tag="ACHOS5_001975"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9776793.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26738..27385)
                     /gene="fucA"
                     /locus_tag="ACHOS5_001976"
     CDS             complement(26738..27385)
                     /gene="fucA"
                     /locus_tag="ACHOS5_001976"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP9776794.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27932..29248
                     /gene="fucP"
                     /locus_tag="ACHOS5_001977"
     CDS             27932..29248
                     /gene="fucP"
                     /locus_tag="ACHOS5_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9776795.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            29281..31056
                     /gene="fucI"
                     /locus_tag="ACHOS5_001978"
     CDS             29281..31056
                     /gene="fucI"
                     /locus_tag="ACHOS5_001978"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP9776796.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPCL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31165..32583
                     /gene="fucK"
                     /locus_tag="ACHOS5_001979"
     CDS             31165..32583
                     /gene="fucK"
                     /locus_tag="ACHOS5_001979"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9776797.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISTQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32585..33007
                     /gene="fucU"
                     /locus_tag="ACHOS5_001980"
     CDS             32585..33007
                     /gene="fucU"
                     /locus_tag="ACHOS5_001980"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP9776798.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33065..33796
                     /gene="fucR"
                     /locus_tag="ACHOS5_001981"
     CDS             33065..33796
                     /gene="fucR"
                     /locus_tag="ACHOS5_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP9776799.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(33840..34940)
                     /gene="rlmM"
                     /locus_tag="ACHOS5_001982"
     CDS             complement(33840..34940)
                     /gene="rlmM"
                     /locus_tag="ACHOS5_001982"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP9776800.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34933..35328)
                     /locus_tag="ACHOS5_001983"
     CDS             complement(34933..35328)
                     /locus_tag="ACHOS5_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP9776801.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35347..36264)
                     /gene="gcvA"
                     /locus_tag="ACHOS5_001984"
     CDS             complement(35347..36264)
                     /gene="gcvA"
                     /locus_tag="ACHOS5_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP9776802.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         8884 a         9662 c         8748 g         9000 t
ORIGIN      
        1 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt ttcttctttt
       61 ttactttcac caccattatg gtctttacct gtttatgtgt acttcagaaa aaagcatctg
      121 gtagaagtta tcattcagac aacagcggtt ccggtggagg ctcaagtggc ggcggattta
      181 gcggcggggg cggttcttct ggcggtggcg gcgcgtccgg tcgctggtaa ctcaaccata
      241 aaaaacgcca gcccgaaggc tggcattttt aaatcagata aagtcagact tatgcctggc
      301 ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa
      361 tcagctggtt gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc
      421 ctgcagcagt accaacagcc aggtcagcga tggtagcgtc ttcagtttcg cctgaacggt
      481 gagagataac tgcagtgtag ccagcgtctt tcgccatctt gattgcagcc agagtttcgg
      541 tcagagaacc gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac
      601 cttctttcag gatcttggtg ttggttacga acaagtcgtc accaaccagc tggattttgt
      661 cgcccagaac tttggtctgg tatgcgaaac catcccagtc agattcgtcc agaccgtctt
      721 cgatggaaac gatcggatac tgtttggtca gttcttccag gaagtgagtg aattcttcag
      781 aggtgaatgc tttgttgcct tcgccagcca gaacgtattt accgtctttg tagaattcag
      841 aagctgcgca gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa
      901 cagcttcagc gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac
      961 cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga
     1021 aaacttcaga acccatacgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca
     1081 tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca
     1141 tcggaaccgg catagagtat ttgcccggag tgccgttcag ttcagcgatg tgctcgtaca
     1201 gcggcatacc tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg
     1261 cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa
     1321 tgccagcctg atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg
     1381 cagcaacagc tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt
     1441 ccagagcttc acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac
     1501 caccctccag atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac
     1561 gaccgatgat ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa
     1621 actccagaca aacaacgcgt acccagggta cgcgttgccg ttctaacttt tttacttact
     1681 tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac
     1741 catcacgtgg agtagaagta aactccggat ggaactggca agccacgaac cacggatgat
     1801 tcggaacttc gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac
     1861 ctgcatcttc aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct
     1921 caacaattgt cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact
     1981 gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt
     2041 tgccgttttc atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca
     2101 caaattccgt agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg
     2161 ccacctgcat acccaggcaa atgcccagat aaggaatatt gttctcacgc gcataacgcg
     2221 cggtcgtaat catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcat
     2281 ccagaccttt aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga
     2341 tgttgacgct gacacgattc ttcagcccac cgtgtttcag tgcttcgatc acggatttgt
     2401 atgcatccgg cagctcaatg tatttgccga ccataccaat ggtgacttca cttaccggat
     2461 tcgcttcttc gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc
     2521 tgaatcgttt acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat
     2581 agatggaatc gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg
     2641 caatcttcgc acgttcgttc gccggaacgg cgcgatctga acgacaaatc aggatatcag
     2701 gctggatacc gattgaaagc agctctttta cagagtgctg agtcggtttg gttttgactt
     2761 caccagacgc tgccatgtac ggcaccagcg tcaggtgcat aaacagggtg tgctcacggc
     2821 caatttcaac agccatctgg cgaatcgctt cgaggaacgg cagggattcg atatcaccta
     2881 cagtaccgcc gatttcgacc agtactacgt catgaccttc gccgccttcc agcacgcgct
     2941 ctttgatagc gttagtgatg tgcggaataa cctgtacggt tgcgccgaga tagtcaccgc
     3001 ggcgttcttt acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc
     3061 tcattttggt acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt
     3121 cttcagtaac gaacacttcc ccgtgttgga ttgggctcat agtaccggga tcgacgttga
     3181 tgtacggatc cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga
     3241 gggaggctgc ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat
     3301 agttcgttgt catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac
     3361 gggaaagcag tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg
     3421 ccagcgcact attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac
     3481 gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatcg cgcttgaccg cgtaattccc
     3541 ttagagatca atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc
     3601 aacacctgtc atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg
     3661 ctcgaatttt tcgttcgctt tttgcaatgc gatttccgct tttgtcccta aatgacgagc
     3721 cagattgacc gtggcaaaca gcaggtcacc catttcctcc tccagtttag cctggtcgac
     3781 aacagcctgc cgcgcctcgt acatcacctc gtcgatctct tcgtagactt tatctactac
     3841 cggaccaagc gtcgtccagt cgaaaccaac gttggcgcaa cgtttctgga ttttttgcgc
     3901 acgcattaaa gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg
     3961 cgcgcgctct tcggttttga tttgctccca acgggcaagc acttcactac tattttcggc
     4021 agaactgtca gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca
     4081 aatatcatta aagtcaaagc gcccttcttc ctgagccatt tgtgcgtaaa acaccacctg
     4141 gaacagcaga tcgcccagtt caccgcgcag atcgtcaaaa tcttcacggg cgatggcgtc
     4201 cagcacctcg taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt
     4261 atcccacggg cagccgtttt ccgggtcgcg caggcgctgc atgatggtga gcaaacggtc
     4321 gatttgattc attgaattgt cctgaaaatt gcgggtccgt caggtgtaaa cctgtgacca
     4381 gaatagaagt gagttagtaa cacctaccca atcagcacgt taattttggc tttaatgagt
     4441 tgtaattcct ctggggcaac tgttcctttc ttcgttgctc ctcttgcccg ccaggcgata
     4501 ctttttacct gatcagctaa cgctacgcca tcacgttcct gaccggatag aacaacttcg
     4561 aacggatatc cttttgattg cgttgtacaa ggaacacaca gacacatacc tgttttgttg
     4621 ttgtacatga acggactcag gacaacagcc ggacgatgtc cggcttgctc gctacctttt
     4681 gtcgggtcaa aatcaaccca aatcagatcg cccgtatcgg gtacgtatcg gcttaccatt
     4741 accagacttc cttatctttc ggctcccccc agtcgatatt ctcgtggagg ttttccggcg
     4801 tgatgtcgtt gaccagttca gcaagcgtaa atacgggctc tttacgcact ggctcaataa
     4861 ttaatttgcc atccaccagg tcaatcttca cttcatcatc aatattcaga ttgagcgcct
     4921 gcattaacgt agccgggatc cgcaccgccg gtgaatttcc ccaacgcttt acgctactgt
     4981 ggatcataac cctttcctca aaccgcgatc atatgtagat acagtatata tcaatctaca
     5041 ttgtagatac gagcaagttt cggcctaact cccgtgcaac cgacgcgcgt cgataacatc
     5101 cggtacctgg ttgagtttac ccagcacgcg acccagcact tgcaggttgt aaatctcaat
     5161 ggtcatgtcg atggtcgcca gttgctgttt ggtgtcgcta cggctggcaa cgccaagtac
     5221 gttcaccttc tcgttggcga gaatggtcgt gatatcacgt aacaacccac tacgatcatt
     5281 agccaccacg cggaccacca gcgaatatcc ggcggagtag ctctcgcccc ataccgcgtc
     5341 aacaatgcgt tctggcgcat gggagcgcag ttccgccaat tgttcgcaat cggcgcggtg
     5401 tactgaaata ccgcgcccct gggtaatgaa gccgacaatc tcatctccag gaatcggctg
     5461 gcagcagcgc gcgatgtggt gcatcaggtt accgacacct tcaaccacca cacgaccgtt
     5521 atctttactg cggttttgcg gcgtgtagct tttttgctga agctgtttca gcgcggcggc
     5581 gtcctgctct tcggcactcg gcttattaaa ttgcgattgc aggaagttca ccatctgatt
     5641 aagacggata tccccgccac caatcgccgc cagcaactcg tcgacatcat tgaagttgta
     5701 acgcggcagc agatgttttt ctgcttcttt caggctgatc cccagatgtt ccagctcgtc
     5761 gtcaaggatt tgccgcccag ccagaatgtt tttgtcacgg tcctgtttac ggaaccaggc
     5821 gtgaattttc gaacgcccac ggctggttgt gacgtaaccg aggtttgggt ttaaccagtc
     5881 acggctgggg ttcggctgtt tctgggtgat aatttcaatc tggtcgccca tctgcagctg
     5941 gtaggtgaac ggcacaatgc gcccgccaat ttttgccccg atgcagcggt gtccgacatc
     6001 actgtggatg tggtaagcga agtccagcgg cgttgatccc gcaggcaaat caacgacatc
     6061 acctttcggc gtaaagacgt acactcggtc gtcaaagacc tgactacgta cttcgtcgag
     6121 catttcgccg gaatcagcca tctcttcctg ccacgcaatc agtttacgca gccaggcaat
     6181 ccggtcttca tgtcccgaac gtgcgccgcc agcagccgcg ccctctttat atttccagtg
     6241 cgcagcaaca cccaactctg catcttcatg catctgtttg gtgcggattt ggatctcaac
     6301 ggttttgcca cccggcccca gaaccacggt atgaatagac tgataaccgt ttggtttcgg
     6361 gttagcgacg taatcgtcaa actcatccgg caggtggcga tagtgagtgt gcactatccc
     6421 cagtgcggca taacaatcct gtaaacgctc ggcgacaata cgtaccgcac gcacatcaaa
     6481 cagctcatcg aaggcgaggt tctttttctg cattttgcgc cagatactgt agatgtgttt
     6541 cggtcgacca tacacttcag ctttaacgcc ttcagctttc atctcagcgc gcagatggcc
     6601 gacaaactct tcgatatagt gttcgcggtc gagacggcgt tcatgcagca gttttgcgat
     6661 gcgtttgtat tcggtcgggt ggagataacg gaagcagtaa tcttccagct cccatttcag
     6721 ttgcccaatc ccaagacggt tcgccaacgg cgcgtagata ttggtgcact cttttgccgc
     6781 cagtacacgt tcatcttccg gcgcatcttt tacttcgcgc agatgagcaa tacgctccgc
     6841 cagtttgatg actacgcagc gaaaatcatc gaccatcgcc aataacatcc ggcgaacgtt
     6901 atcgacctgt tcggaggaaa cagaatcagt gtgcgtcgct ttcagctggc ggatcgccgc
     6961 catatcacgc acgccgtgaa taaggttaac gaccgactta ccgacgctat cacgcagcac
     7021 atcttcgctg actacgttgg catcggccag agggaacagc agcgccgccc gcagcgtgtc
     7081 aatgtccata cttaatgtcg agaggatctc caccatctca acaccacgcc acaataacag
     7141 actggcatcc ggatgcccct gcgtctgttg cagacaatac gcccaggttt cggctaagca
     7201 ctcacacgac ttctggctgg taatacccag acttgcgatc catttttccg gatcaaattc
     7261 accagcctta ttgatatgtg cacttcttac cgcaaccatc gtcctctcct ttagggacca
     7321 gacctgccga aatcggcaaa tcgtaactat ttaacgcgcg agaaaagtac catcgattcc
     7381 agatgtcccg tgtgtgggaa catatccagc atcgccagtc gcgcaatggt atatcctgct
     7441 tttaataacg cttcgctatc ccgagccagc gttgcagggt tacaggatac ataaactata
     7501 cgaataggtt ccagttttat aatttgctgc ataacacctg cggcacctgc tcgcgccggg
     7561 tccaacaaca ctttatcgaa gccgtttttc gcccacagct gctttgtgac atcttcttca
     7621 agattttcgt gataaaacgt cacattctgt aagccattaa gacgcgcatt ctgctggcct
     7681 ttttccacca gcgccggaac accttctaca cccaccacac tggcagcttg tgtcgccaat
     7741 ggcagtgtaa agttgcccat accgcagaac agatccagta cgcaatcttc aggttccaca
     7801 tccagccatt ccaacgcacg cgctaccatt ttctggttca cacccgcatt gacctgaata
     7861 aaatcgcgcg ggctaaaagt taagcgcaac ccatttgagt cataccaggg catctcacca
     7921 gagacggttt cgagtatctc actatcgggg gcgagataca gatccaggcc ttcagaatgc
     7981 gaaaagcatt ccagtttttc gcgatctgcc gaacttagcg gtgcggtatg gcgcaaaatc
     8041 attagcgtgc cgctggttgc ctgtaccagt tcaacatgac caagatggcg catagcttgt
     8101 aagctgccca ggcatgccct gactttgggc agcaatgctt caagttgggg cactaaaatg
     8161 gggcattgtt tcacgtcgac aatgtcactg gagcccgctt tgcgaaaccc catctgaagt
     8221 tgctgtgttt tcggtaagta gttcagactt aaacgcgcgc gacggcgata accccaggga
     8281 acatcggcga tcacttcaga gacttcgtgt ttcattaatc gggcgagtgc cgcacttttg
     8341 cttcgctgct gtaaatccac actggcgtgt tgttgctgac agccgccgca tacgccaaaa
     8401 tgaggacagc gtggcgtttc gcgttccggg ctatcgctta accgacgtac gactttagcg
     8461 cgggcatact gttttttatc ttcagtaaca gtaacttccg cgttttcctg cggcaataat
     8521 ccggggataa acaacgtttt gccgttatgt cgcgccacgc cctgaccaaa agagtcgagg
     8581 tcgttgactg aaacggttat gatctgacgc gtcgtcgtgc gtcgttttgc agagtagaat
     8641 tgcgccattg gcgagacttt ctcaatttaa cagtgtgacc ttaattgtcc cataacggaa
     8701 ctccatgacc aactacagcc tgcgcgcacg catgatgatt ctgatcctgg caccgaccgt
     8761 ccttattggt ttattgctga gtatcttttt cgtcgtgcat cgctataacg acttgcagcg
     8821 tcaactggaa gatgccggtg ccagcattat tgagccgctt gcagtttcta ctgaatatgg
     8881 catgagtctg caaaatcgcg aatctatcgg tcagttaata agcgtactgc atcgtcgcca
     8941 ttctgatatt gttcgcgcga tttcggttta tgatgaaaat aaccgactct ttgtcacctc
     9001 caattttcat cttgatccct catcaatgca gctcggcagc aacgtgccgt ttcctcgcca
     9061 gctcactgtc actcgtgacg gcgatattat gatcctccgc acgccgatta tttctgaaag
     9121 ttactccccc gacgaatcgc ccagtagcga tgccaaaaat agtcaaaata tgctgggata
     9181 tattgcgctg gagctggatc ttaaatcggt tcgcttgcag caatataaag agatctttat
     9241 ttccagcgtg atgatgctgt tttgtatcgg tattgcgctt atttttggct ggcgcttaat
     9301 gcgcgatgta accggtccga ttcgcaacat ggtgaatacc gtcgaccgca tccgtcgcgg
     9361 gcaactcgac agccgggtgg aaggatttat gctcggcgag ctggatatgc tgaaaaacgg
     9421 tatcaactcg atggcaatgt cgctggcggc ttatcacgaa gagatgcaac acaatatcga
     9481 ccaggcgacg tccgatctgc gtgagacgct ggagcaaatg gagattcaga acgttgagtt
     9541 ggatctggcg aaaaagcgcg cccaggaagc ggcgcgtatt aaatccgagt ttctggcaaa
     9601 tatgtcacac gagctgcgta caccactgaa tggtgttatt ggctttaccc gcctgacgct
     9661 gaaaacagaa ttaacaccaa cgcagcgcga tcacctgaat acgattgaac gttcggcaaa
     9721 taatttgctg gcaattatta atgatgttct cgacttctcg aaactggaag ccggtaagct
     9781 gattctggaa agtattccat tcccactacg cagcacgctg gatgaagtcg ttaccctgct
     9841 ggcacattct tctcacgata aagggctgga actgacgctc aatattaaaa gcgacgtgcc
     9901 tgataacgtg atcggcgacc cactacgatt acagcaaatc atcactaacc tggtagggaa
     9961 tgcaattaaa ttcaccgaga atggcaacat tgatattctg gtagaaaaac gtgcgctgag
    10021 taataccaaa gtgcaaattg aagtgcagat tcgggatacc ggcattggta ttcctgaacg
    10081 cgatcaatcg cgcttattcc aggccttcag acaggctgat gccagtattt cccgccgtca
    10141 tggtggcacc gggctggggc tggtgattac acaaaaactg gttaatgaaa tgggcggcga
    10201 tatttcgttc catagcctgc cgaatcgtgg ttcaactttc tggttccaca ttaatctcga
    10261 tctgaacccg aacattatta tcgaagggcc atccacccag tgcctcgctg gtaagcgcct
    10321 ggcctatgtc gaaccaaact ccgcagcagc acaatgcacg ctggatattt taagtgaaac
    10381 gccgctggaa gtggtttata gcccaacgtt ctccgcgctg cctcccgcgc attacgacat
    10441 gatgttatta ggcatagcgg tgaccttccg cgagccgcta acaatgcaac atgagcgatt
    10501 agcgaaagca gtatcgatga ccgatttcct gatgctggca cttccttgcc atgcacaagt
    10561 caatgctgaa aaacttaagc aagatggtat cggcgcgtgt ctgctgaaac cattaacacc
    10621 tacgcgcctg ttgcctgccc tgacggaatt ttgtcatcac aaacaaaaca cgcttttgcc
    10681 tgtaaccgat gaaagtaagc tggcaatgac agtcatggcg gttgatgaca accccgctaa
    10741 cctgaaactt atcggcgcat tgctggaaga tatggtgcaa catgtggaac tttgcgatag
    10801 cgggcatcag gcggttgaac gggcgaaaca gatgccgttc gatttaatct taatggatat
    10861 tcaaatgccg gacatggacg gcattcgggc atgtgagctc atccaccagc tcccgcatca
    10921 gcagcaaacg ccggttatcg cggtaacggc gcatgcgatg gccgggcaaa aagagaaact
    10981 gcttggcgcg gggatgagtg attatctggc caaaccgatc gaagaagagc gattgcataa
    11041 tttgctgttg cgctacaagc ctggcagcgg tatttcctct cgcgtcgtga cgcccgaagt
    11101 caacgaaatt gtggtcaacc cgaatgcgac cctcgactgg caactggcac tacgccaggc
    11161 agcaggaaaa accgatttag cgcgcgatat gctgcaaatg ttactcgatt tcctgcctga
    11221 agttcgcaac aaagttgagg aacagctggt tggagaaaac ccggaaggcc tggttgattt
    11281 gattcataaa ctgcatggca gttgcggcta tagcggtgta ccgcgtatga agaatctctg
    11341 ccaactgatc gaacaacagc tacgtagcgg tactaaagaa gaagatttag agccggagct
    11401 gctggaactg ttggacgaga tggataatgt agcgcgcgaa gccagcaaaa ttctcgggta
    11461 atggacgtct tgtcgagtag caaccggatt tttatgacgc tggtttcaga ttggtgacaa
    11521 gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc cggcctgcaa aaccaattaa
    11581 gttcaatgaa ttaaaaaatg atgtaaccct gatacgtagc gcaacaggca attttgcgtt
    11641 tggcatcagt ctcaaaccgg ctccagatag agccggtttt ggttttctgt cttaacgcac
    11701 catgcacgga cgcttgttat cgaacgtcca gccggggatc aggtactgca ttcccatcgc
    11761 atcgtcacgc gcgccaagcc cgtgtttctg atacagctcg tgcgctttca tcacttgatc
    11821 catatcgatt tctacaccca gccccggttt ttctggcacc tgaaccagac cgcctttgat
    11881 ctctaacggt tctttggtca ggcgctgatt accttcctgc cagatccagt gcgtatcaat
    11941 cgcggtgatt ttacccggtg cggcagcggc aacatgggta aacatcgcca gagaaatatc
    12001 gaagtggttg ttggagtgtg aaccccaggt caggccaaat tcatgacaca tttgcgccac
    12061 acgtaccgaa ccttgcatcg tccagaaatg cggatccgca agcgggatat caacggattg
    12121 caacgagagc gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg
    12181 caggccagtt gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc
    12241 accacacgga tcttctgcat aagccagcga acctttcagg tatgtaccga ttttaatcgc
    12301 ttcgttcagc gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaaacgttg
    12361 cgccagtgcc acaatagatt cggcttcttc ttccccggcc agtacaccgc ctttcagttt
    12421 gaaatcgttg aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg
    12481 cgtcatcgct tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg
    12541 atacggcagc ggtgtagctt tgcgattgcc gacgaagaac agataaccga gcatttcgac
    12601 ttcgctacgc tgttgaccat cgcccagcag cgatgccacg tttaccccca gatgctgccc
    12661 cagcagatcc agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc
    12721 aaatgtctgc aaaccgcgcc caccggcatc acgatcggca aaagtattac gcaccagcgt
    12781 cagaacgttt ttgtattcac ccagcgtttt acccaccacc agcggaatcg catcttccag
    12841 cgttttacgg attttctcgc cgccgggaat ttcccctacg ccagtgtgac cggaattatc
    12901 tttgataatc acaatattac gcgtgaagaa cggtgcatga gcaccactca gattcatcag
    12961 catactgtca tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa
    13021 ttgagaactc atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta
    13081 cggtcaaagg tccagccggg gatcaggtac tgcatcgggc ctgcgtcgtt acgcgcaccg
    13141 ccaggcagac gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg
    13201 cccagaccgg gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg
    13261 gtcagacgac aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc
    13321 ggtgccgccg cgcccacatg ggtaaacatc gccagagaga tatcaaagtg gttattagaa
    13381 tggcagcccc aggtcagccc ccagtcgtcg caaagttgtg ccacgcggac tgcaccggaa
    13441 agcgtccaga agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga
    13501 cccatttcgc gccagttggt ggcgatcatg ttggtcgcga cgggcaagcc ggtcgcccgt
    13561 cgaaactccg ccatcacttc acgaccggag aaaccctgct cagcgccgca cgggtcttcc
    13621 gcataggtaa gaacatcatt tagccctttg cacagagaaa tggcttcatc aagcagccag
    13681 gcaccattgg gatcaacggt aatccgcgca tcagggaagc gtttcttcaa tgtacgaaca
    13741 gtgtcgattt cttgctcgcc aggtaacacg ccacccttaa gtttgaaatc tttaaagcca
    13801 tagcgatcct gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga
    13861 tgccgcaact gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt
    13921 ttggtccgat caccgatata aaacagataa ccgagaacgg taatagcatc acgttgcttg
    13981 cctggcccta acagttcgca gaccggaaca ttcagcgcct gacctaacag gtcaagcaag
    14041 gcggcttcca gcgccgccac ggcattaacg cgcaattcaa aagtccaggc acctttgccg
    14101 aaggtatcaa aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg
    14161 cgcgcaactt cctggcccag caccatcgga atagccttga ccagtgtttg ataaatcacc
    14221 tctccgcccg gcgcttcacc aatgccggta tgcccggcat tatcggtgag taccacaata
    14281 ttgcgggtga aatatgcgtt atgtgcacca ccaatattaa gcaacatgct gtcatgtccg
    14341 gcgaccggaa tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg
    14401 ccccgcgaca ggtttcagct caatacgctt gatatcgccc accagcacca ggtagctgag
    14461 taccgcgatt aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt
    14521 gccaacgata taaccaattg cgattggcgt gacgatgcca gaaatgttac caaacatgtt
    14581 aaacaggcca ccggaaagac cgctgatctc ttttggcgca gtatcagcca ttacagccca
    14641 acccagcgca ccgatcccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca
    14701 ctcaacgttg acgtagttgc agaacaccat caccatcgac aacaacatgc ccatcacgat
    14761 cggtgtttta cgcgcaatgt tcagtgaacc cgtgcgacgc atcagccagt cggaaataat
    14821 cccacccagc acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc
    14881 tttcagaatc gacatcccgc gcgcctgcac cagataaacc gggaaccagg taatgaaaaa
    14941 gtaagtcagg gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc
    15001 gagcagctgt ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg
    15061 ctgatccata ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac
    15121 gcccggatgt tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatgcc
    15181 cagaccgccc ataaagaaga agacgtgtga ccagcccact tcatgagtca accagcccat
    15241 aatcggcgcg aagataaccg ttgcgaagta ttgggcggag ttaaaaatcg acaccgccgt
    15301 tcccctttcc tgcgccggaa accaggccgc aacaatgcgg ctgttgccag ggaaagaggg
    15361 cgcttcagca agcccgacca ggaagcgcag tgtaaacagg gcaacgataa tgccgaatcc
    15421 actaaagata tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca
    15481 gaagtagacg cgttttgaac caaaacgatc cagcaaccag ccaccaggga tctgcccgat
    15541 aacatacgcc catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat
    15601 atctttggcc atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga
    15661 tgtgacgata aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact
    15721 gctcgcagcc tgacttaaag aactcattgt tgcactcctg aaaattcgcg ttagccacgc
    15781 tcactctgga ctgcgacatc gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt
    15841 tttataggtc gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag
    15901 cgcgccatag cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt
    15961 ttggcattag ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag
    16021 gaagggttaa aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa
    16081 cgccagtaaa aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga
    16141 ttcagcaccg ctaaaacggc atttaccgct cgctgaacat atcaggacaa catcgtgccc
    16201 caatgttcaa cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc
    16261 aacagcattt cgccaacccg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg
    16321 ccgccattgc agaaattcac ataactactg tcgccgagcg caatcacgcc ataacgcaga
    16381 ttcggctgga agcccagact atctttgatt ccctgaaaga gtggcacaat gctatcagga
    16441 aggtcgccct gcccggtcgt ggacgtaacc accagaacat acttatcctg atagggcagc
    16501 cagtcgctta attcaggatc ttcaaatacc gttgctttgt ggccctgcgc ggtcagaatc
    16561 gcttccgctt cttcggccac taacagtgaa ttcccgtaca tggtgccgac aaaaatacca
    16621 atttccgcca taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg
    16681 gcgcactgaa ctcaaccctt tcattttcag gaagcagacc ccgccagcca aattgtgata
    16741 acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag
    16801 gatgcgtcaa tgacaactga ctggcatgca gcattaaccg ctgaaggcca aaatgctcag
    16861 caccgctgcg attctggcgt aaatcaccat gtttgctgtc gccaataatc gggtgccgca
    16921 aatgggcaag atggcgacga agctggtgct tacgtccggt tttcggctcc agttctacca
    16981 ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac
    17041 cgcgataatg cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat ttatcagcga
    17101 ttttgtccag ttcttccacc agtggataat ccagcaccgc ttcttccatc aaccagccgc
    17161 gcacaatcgc atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc
    17221 gtccggcctc gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat
    17281 gagcagtaaa aacatgctgg cctatctggt cacgcacggt ttgcatgacc actactttct
    17341 cgtcgcgatc cagccagctg cggtgaacca gccagccgga gggtttattt accgcaacca
    17401 gccattcatc ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc
    17461 cagtttctcc agctcagcca aaataagcgc gcgttgcgga tgatcagtcg ccagcgccat
    17521 ttcataatag ggcgcaacag caaaagcgcc cggtaacggc tggttgttat ctaacaaatc
    17581 gtgcattcgc gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa
    17641 gaacggttga gtactgttaa attgatgcgg caggggttca tcgtttcgcc agtgctgatg
    17701 ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat
    17761 aaagcaacct caataagaaa aactgccgcg aagaatagca ggaataaaaa aagggagcac
    17821 tgtattcaca gtgctcccgg ttcgtttcgc agcattccgg ctacttttgt tgctccctgc
    17881 tcatccttga caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt
    17941 cctgctcaca ccaccccgat gtgatacttc atcctgaagt gttcctggcc ttcctgaccc
    18001 accgaatcat cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg
    18061 cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctt
    18121 cagaagtgtc atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga
    18181 atcgtctttt tctcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca
    18241 tatgaaagtt aagaattaac aagcaacatg ttgtttttaa atgagaataa cgattcttat
    18301 ctttttaagt attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca
    18361 caaccagatg gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag
    18421 atttgaggct aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga
    18481 gagattacac accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg
    18541 cggcagtttt agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg
    18601 gaagtcgtct tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg
    18661 catatccccg gcaaactgag tcgtataaaa ggtatgaatc gtgggttgta tcacaatatc
    18721 aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta
    18781 tacggcatct tcggtagttg aaataatgca cggtgaagga acgccgtaca gttcctcact
    18841 taacggccag cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt
    18901 cagggcttgt gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt
    18961 gtcgtttagt ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc
    19021 acttgcgggc ctgacacttg gaaaatcaac cgattaccgg gatacctatg acgccagcct
    19081 actgcaaggc gtaccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct
    19141 gccttttcac ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg
    19201 tttgccgcag gtcgctgtcg gtcatgttga acttgattac accagcgtaa atctgattga
    19261 gtcgaagagt tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga
    19321 tgaggtgtgc cagacgctgg agcgcgactt aagcacttgc gcccagggta aggttagcgt
    19381 ggcgttatat cgtcttgatg aactggaagg ccagccgata ggtcatttca atggcacctg
    19441 cattgatgac caggatatca ctatcgataa ctatgaattc actactgact atctggagaa
    19501 tgccaccagc ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa
    19561 ctgcctgatc acccatcaac cagattgggg ttccatccaa attcagtatc gtggacgcca
    19621 aattgacaga gaaaaactgc tgcgttacct ggtctctttc cgtcatcaca acgagttcca
    19681 cgaacagtgc gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt
    19741 gagcgtttac gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa
    19801 taacgatttt gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg
    19861 cgtgctggat tcacgcagaa ggttgtgaaa ggtcagcagg cagggctatt gtaatcaaag
    19921 ggaatgacga tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg
    19981 ctccatggat atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga
    20041 cctctatcaa ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga
    20101 taacagcaaa gaattgctgt ctcgttttga aaatttcgat attaacgtcc tgcgccgtga
    20161 acgcggcgta aagctggaac tgataaatcc cccggaagag gcttttgtcg atgggcgaat
    20221 tattcgcgct ttgcaggcca acttgttcgc ggttctgcgt gacattctct tcgtttatgg
    20281 gcaaattcat aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac
    20341 taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg catgtcggtg aagcgccaaa
    20401 tatggtggtc tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg
    20461 cgtcgggaat cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc
    20521 gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag
    20581 tcgctttatt ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt
    20641 caacgaattg atcatcatgc cggatatcga aaaacgtctg gaagcgtttg tccgtatcgc
    20701 tcacggtatc attatcttcc ctggcggtgt gggtacagca gaagagttgc tgtatttgct
    20761 gggaatttta atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc
    20821 gaaagagagc gccgactact tccgcgtact ggacgagttt gtcgtgcata cgctgggtga
    20881 aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat
    20941 gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgcgac actggtgacg cttacagctt
    21001 taactggtca atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa
    21061 tatggctaat ctgaagcttt acccggatca acccgttgaa gtgctggctg ccgacctgcg
    21121 ccgtgcgttc tccggtattg tggcgggtaa tgtaaaagaa gtcggtattc gcgccattga
    21181 agagtttggt ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct
    21241 gctacagggt tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg
    21301 ctacgaaatc tgcacataat ctcccctccc ccggtgacgt ttgttgccgg gttttctcat
    21361 ttttggtcac ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc
    21421 acgcgacaaa tatcaccaca gttaatatgt catacaattt atgttgaaac gcaaacgttt
    21481 ccctattttc ataaaccata ttttattgcc atgaaaaaga aattatcgcc atgattgact
    21541 aaaagtattg attttttcag ttcaacctac atatattgcg cgccccgtaa gaagtcagat
    21601 gtcgtttaat gggtaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa
    21661 gcgttgtgat tatctatctt ccaagtagat tattgtattt gggatcaaga tcactgatag
    21721 atacataact tgtgtgtatc tttccgccct caaattatta cggtggaaaa tgattaagcc
    21781 atcgccgata gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa
    21841 aagctgaatt atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga
    21901 ttgcgtcgaa agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc
    21961 tttacggcac ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgct ggtgttggcg
    22021 gtatgatccc gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc
    22081 gcggcctgac tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg
    22141 tagaagaaca ctttggtatt ggcgcaggta aactgattac tctgctctac ttcttcgcta
    22201 tctacccgat cctgctggtt tatagcgtgg caatcaccaa tacagttgaa agcttcatgt
    22261 ctcaccagct gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg
    22321 gtatgatgac catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg
    22381 tattcccgtt tgttggcgta ctgatgctgc tggctctgta cctgatcccg cagtggaacg
    22441 gcgcagcact ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga
    22501 tgacactgtg gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct
    22561 cttctttcgc cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga
    22621 agatcctggc attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct
    22681 gcgtactgag cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc
    22741 tgtcttacct ggctaaccac tttaacgcgc cgatcatcgc gtggatggct ccgattatcg
    22801 cgattatcgc tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca
    22861 acggtatggt gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga
    22921 accgtatcac tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga
    22981 gcatcctggg tatgattgaa accctgggcg gcccaatcat cgcgatgatc ctgttcctga
    23041 tgccgatgta cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca
    23101 acgtattcgt tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca
    23161 gctaagtcct ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc
    23221 ctatgattag cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg
    23281 ttggaccaat gaaagcgggt aaacaattta ccgacgatct gattgcccgt aacctgctta
    23341 aagacgtgac ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc
    23401 accacacaga tatcgccata attatgggtc tggcgggtaa cctgccggat accgtggata
    23461 tcgattccat ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa
    23521 acggtcagca tgaagtggag ttcccggttg atcagtgcat gaacttccat gccgacaacc
    23581 tttctctgca tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca
    23641 gccagactta ttactctatc ggcggtggct ttattgttga tgaagaacat tttggtcagc
    23701 aggatagcgc accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac
    23761 attgtcagga aaccgggctg tcactctctg gcctgatgat gaaaaacgag ctggcgctgc
    23821 acagcaaaga agagctggaa cagcacctgg cgaacgtctg ggaagtcatg cgtggcggta
    23881 ttgagcgcgg tatttctacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg
    23941 ctgcggcact acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg
    24001 ttgttgactg gatcaacatg tttgcactgg cagtgaacga agagaatgct gctggcggtc
    24061 gtgtggtgac tgcgccgact aacggtgcgt gcgggattat tccggccgtg ctggcgtact
    24121 acgacaagtt tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca
    24181 gcgccattgg ttctctttat aagatgaacg cgtcgatttc tggtgccgaa gtcggctgcc
    24241 agggtgaagt cggcgtggcg tgctcaatgg cggcagctgg tctggcagaa ctgttaggcg
    24301 caagcccggc gcaggtatgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga
    24361 cgtgtgaccc ggttgccgga caggtccagg taccgtgcat cgagcgtaac gccattgcgg
    24421 cagtaaaagc ggtgaacgcc gcacgtatgg cactgcgccg taccagcgag ccgcgcgtct
    24481 gcctcgataa agttatcgaa actatgtacg aaacaggtaa agatatgaac gccaagtacc
    24541 gcgaaacctc tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca
    24601 gaggcctctt tttgcgaggc ctcttcccga tttctcatcc agccatagcc tgttctggca
    24661 tcgaatgtta cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta
    24721 ttgtcgatgc actgaatctt attcgtcgca ttcatgccgt tcagggttcg ccctgtgtcg
    24781 aaacctgcca gcacgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg
    24841 ccgtttttga tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca
    24901 aagcgggtcg accaccaatg ccggaagagt tgcacaacga gatgcctgca ttacgcgccg
    24961 cctttgagca acgcggcgtc ccgtgctggt cagccagcgg taacgaagcc gatgacttag
    25021 ccgccacgct ggcggtcaaa gtgacacagg ccgggcatca ggcaacaatt gtttcgacag
    25081 ataaaggcta ctgccagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac
    25141 gttggctgga tgcaccattt attgataaag aatttggcgt tcaaccgcag cagttacccg
    25201 attactgggg gctggcgggg atcagcagtt caaaggtgcc aggcgttgcg gggattggcc
    25261 ccaaaagcgc cacgcagttg ctggtcgagt ttcagagtct ggaagggata tatgagaatc
    25321 tggatgcggt tgccgaaaag tggcgcaaaa aattagaacc ccataaagag atggcgtttc
    25381 tgtgccgcga tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat
    25441 tgcggttggt acggtaacgg cgtgccggat gtaaacgatt atcaacacat tgaatttaat
    25501 gcacttatat gccggataag acgcggctag cgtcgcatcc ggcattatca catcagcgca
    25561 tttaccaggc ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc
    25621 caccggtaca aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta
    25681 caccaacatc acgtaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt
    25741 caacagcggc attacgcgcc tcttccaggc tcataccttc cactttcacg cccataacgc
    25801 gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg acatgcggta
    25861 acaggatggc atttgcaaca ccgtgtggcg tgttgtaaaa cgcgcccagt ggatgcgcca
    25921 taccatgcac caaccctaac ccaacattcg agaatcccat acccgcaaca tactgcccga
    25981 gcgccatttc ttctccggca tccttatcac cagcaaccga tcctcgcagc gccccagcaa
    26041 tgatttcaat cgctttaatg tgcagcgcat cggttagcgc ccacgcgcca cgggtaatat
    26101 acccctcaat agcatgagtg agcgcatcga cacccgtcgc ggctttcagc gctggaggca
    26161 taccatccat catgtcagcg tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc
    26221 aaacaaactt gcgccgtttt tcttcgtcag tgatcacgta gttaatggtc acttctgccg
    26281 cagtacctgc tgtggtgggg attgccagaa tcggtacact gggtttattg gtcggggaaa
    26341 gcccttccag gctacgcaca tcggcaaact ccggattgtt gctgataatg ccaatcgctt
    26401 tacacgtatc ctgtggagaa ccgccaccaa tagcgatcag gtaatccgcg ccgctattct
    26461 ggaatacacc gagcccttct ttgacgacag taattgttgg gttgggcact acgccgtcgt
    26521 aaatcgccca tgccagccct gcggcatcca tcttatcggt cactttagcc accacgccgc
    26581 attgcaccag cgttttatcg gtgacgatca gcgccttctg ataaccacgg cgtttcacct
    26641 catcggttaa agccccaaca gcaccccgac caaaccatgc cgtttcgttc agaatcattc
    26701 tgttagccat catccttctc cttgttgctt tacgaaatta ctcttcaatc cgtaacccgt
    26761 aggttttgaa tttctccagc actacggcaa tctcttcatc gctcagcact ggcaccgggt
    26821 ccgtaatcgc cagggtcgtc aggtaaagtt gtgccagcac ttcaacttca tgcgccagcc
    26881 ataacgcttt ctccagattc gcctcacaag cgataagccc atgatgttgt aacaaagttg
    26941 ccttacgatt tttgagagcc agcgcaacat gttcagaaag ttcgcgtgtt ccaaaggtcg
    27001 cataaggcgc gcaaggaata gaattaccgc cagccgccgc aatcatgtag tgaatagcgg
    27061 ggatcggtcg gttaagaatg gaaactgccg tacaatgaac ggcatgattg tgaacaaccg
    27121 cgttggcatc cggtctgctt tgataggccg ccatatggaa acgccactcg cttgagggga
    27181 gttttccttc ctcatgtttg ccgttgccat caataaagac aatatgcgac tccgtcagtt
    27241 tttcatatgg aatacctgta ggcgtaatca gcatcccatc ctgataacgt acactgacgt
    27301 tacccgctgt cccctggttc agccccaggc gagtcatttc caggcaagtg tcaataatct
    27361 gacgagcaag tttatttcgt tccattagct acctctctct gattcaaaac agggcaataa
    27421 tgttgttcct ttcacactat tgaattagcc gtttaattac ccaccatatt cttcctgatt
    27481 aaccagaaag aaattcacaa gcttatattt tgtgacctgg ttcaactaat cacagtaaat
    27541 aactgcaagt tctcttttta taactccatt aaaaatgacc gctcttaaaa atatttatca
    27601 aaacggtcat ttttctactc ctccctgcca ggaatgcccg ttttcggcac aaacaattaa
    27661 tacggtcatc tggtttgtgt tttttatgat ttattttctg aaacgggcat gaaatttcga
    27721 ttattaaagt gatggtagtc acataaagtc accttccggc taataagtgt gaccgccgtc
    27781 atattacaga gcgtttttta tttgaaaatg aatccatgag ttcatttcag acaggcaaat
    27841 attcactgat atgaagcccg aactcgctgg ttttgcactt ttgaaaacat aaccgattac
    27901 gtgcttaagc ttctgaacct aagaggatgc tatgggaaac acatcaatac aaacgcagag
    27961 ttaccgtgcg gtagataaag atgcagggca aagcagaagt tacattattc cattcgcgct
    28021 gctgtgctca ctgttttttc tttgggcggt agccaataac cttaacgaca ttttattgcc
    28081 tcaattccag caggctttta cgctgacaaa tttccaggct ggcctgatcc aatcggcctt
    28141 ttactttggt tatttcatta tcccaatccc tgccgggata ttgatgaaaa aactcagtta
    28201 taaagcaggg attattaccg gattgttttt atatgccttc ggcgctgcat tattctggcc
    28261 cgccgcagaa ataatgaact acaccttgtt tttagttggc ctatttatta ttgcagccgg
    28321 attaggttgt ctggaaactg ccgcaaaccc ttttgttacg gtattagggc cggaaagcag
    28381 tggtcacttc cgcttaaatc ttgcgcaaac atttaactca tttggcgcaa ttatcgcggt
    28441 tgtctttggg caaagtctta ttttgtctaa cgtgccacat caatcgcaag acgttctcga
    28501 taaaatgtct ccagagcaat tgagtgcgta taaacacagc ctggtattat cggtacagac
    28561 accttatatg atcatcgtgg ctatcgtgtt actggtcgcc ctgctgatca tgctgacgaa
    28621 attcccggca ttgcagagtg ataatcacag tgacgccaaa caaggatcgt tctccgcatc
    28681 gctttctcgc ctggcgcgta ttcgccactg gcgctgggca gtattagcgc aattctgcta
    28741 cgttggcgca caaacggcct gctggagcta tttaattcgc tacgctgtag aagaaattcc
    28801 aggtatgaca gcaggctttg ccgctaacta tttaaccgga accatggtgt gcttctttat
    28861 tggtcgtttc accggtacct ggctcatcag tcgcttcgca ccacacaaag tcctggcagc
    28921 ctacgcatta atcgctatgg cactgtgcct gatctcagcc ttcgctggcg gtcatgtggg
    28981 tttaatagcc ctgactttat gcagtgcctt tatgtcgatt cagtacccaa caatcttctc
    29041 gctgggcatt aagaatctcg gccaggacac caaatacggt tcgtccttca tcgttatgac
    29101 cattattggc ggcggtattg tcactccggt catggggttt gtcagtgacg cagcgggcaa
    29161 catccccact gccgaactga tcccggcact ctgcttcgca gtcatcttta tctttgcccg
    29221 tttccgttct caaacggcaa ctaactgaac atattttccg aataaagtga ggaatcagta
    29281 atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg ttattgacgg tcgtcgcatg
    29341 ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg cgaaagccac ggctgcactg
    29401 ctgaccgaga aactgcgcca tgcctgtgga gctgccgtcg agtgtgtcat ttccgatacc
    29461 tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa aattcagcag tcagaatgta
    29521 ggcctcacca ttacggtaac cccttgctgg tgctatggca gtgaaaccat cgatatggat
    29581 ccaacccgcc cgaaagccat ttggggcttt aacggcactg aacgtcccgg cgctgtttac
    29641 ctggcagcgg ctctggcagc tcacagccag aaaggcatcc cggcattctc catttacggt
    29701 catgacgttc aggatgccga tgacacatcg attcctgccg atgttgaaga aaaactgctg
    29761 cgctttgccc gcgccggttt agcagtcgcc agcatgaaag gtaaaagcta tctgtcgctg
    29821 ggcggcgttt cgatgggtat cgccggttcc atcgttgatc acaacttctt tgaatcctgg
    29881 ctgggaatga aagtccaggc ggtggatatg accgaactgc gtcgccgtat cgatcagaag
    29941 atttacgacg aagccgaact ggaaatggcg ctggcctggg ctgataaaaa cttccgctat
    30001 ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg agcaaagccg cgcagttctg
    30061 cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga tgcaaggcaa cagcaaactg
    30121 gccgatattg gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc aggcttccag
    30181 gggcaacgtc actggaccga tcaatatccc aatggcgata ccgctgaagc gatcctcaac
    30241 agttcatttg actggaatgg cgtgcgcaaa ccgtttgtcg tggcgaccga aaacgacagt
    30301 cttaacggcg tggcaatgtt aatgggccac cagctcaccg gcaccgctca ggtatttgcc
    30361 gatgtccgta cctactggtc accagaagca attgagcgtg taacggggca taaactggac
    30421 gggctggcag aacacggcat catccatttg atcaactccg gttctgctgc gctggacggc
    30481 tcctgtaaac aacgcgacag cgaaggtaaa ccgacgatga agccgcactg ggaaatctct
    30541 cagcaagagg ctgacgcttg cctcgccgct accgaatggt gcccggcgat ccacgaatac
    30601 ttccgtggcg gcggttactc ttcccgcttc cttaccgaag gcggcgtccc gttcaccatg
    30661 actcgtgtca acatcatcaa aggcctggga ccggtactgc aaatcgcgga aggctggagc
    30721 gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc aacctggcca
    30781 accacctggt ttgcaccgtg cctcaccggt aaagggccgt ttacggatgt gtactcggta
    30841 atggcgaact ggggcgctaa ccacggggtt ctgaccatcg gccacgttgg cgcagacttt
    30901 atcactcttg cctccatgct gcgtatcccg gtatgtatgc acaacgttga agagaccaaa
    30961 gtgtatcgtc cttctgcctg ggccgcgcac ggcatggata ttgaaggcca ggattaccgc
    31021 gcttgccaga actacggccc gttgtacaag cgttaataca tgactccctg atgtgatgcc
    31081 cggtcgctcc ggctaccggg cctgaacaag caagagtggt tagccggata agcaatgtta
    31141 tccggctata ttgcaggagc gattatgaaa caagaagtta tcctggtact cgactgtggc
    31201 gcgaccaatg tcagggccat cgcggttaat cggcagggaa aaattgttgc ccgcgcctca
    31261 acgcctaatg ccagcgatat cgcgatggaa aacaacacct ggcaccagtg gtctttagac
    31321 gccattttgc aacgctttgc tgattgctgt cggcaaatca atagtgaact gactgattgc
    31381 cacatccgcg gtatcgccgt caccaccttt ggtgtggatg gcgctctggt agataagcaa
    31441 ggcaatctgc tctatccgat cattagctgg aaatgtccgc gaacagccgc ggtaatggac
    31501 aatattgaac ggttgatctc cacacagcag ttgcaggcta tttctggagt cggagccttt
    31561 agtttcaata cgttatataa gttggtgtgg ttgaaagaaa atcatccaca actgctggaa
    31621 cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc accgtttaac cggcgaattc
    31681 actactgata ttacgatggc cggaaccagc cagatgctgg atatccagca acgcgatttc
    31741 agtccgcaaa ttttacaagc caccggtatt ccacgccgac tcttccctcg tctggtggaa
    31801 gcgggtgaac agattggtac gctacagaac agcgccgcag caatgctcgg cttacccgtt
    31861 ggcataccgg tgatttccgc aggtcacgat acccagttcg ccctttttgg cgctggtgcc
    31921 gaacaaaatg aacccgtgct ctcttccggt acatgggaaa ttttaatggt ccgcagcgcc
    31981 caggttgata cttcgctgtt aagtcagtac gccggttcta cctgcgaact ggatagccag
    32041 gcagggttgt ataacccagg tatgcaatgg ctggcatccg gcgtgctgga atgggtgaga
    32101 aaactgttct ggacggctga aactccctgg caaatattga ttgaagaagc tcgcctgatc
    32161 gcgcctggcg cagatggagt gaaaatgcag tgtgatttat tgtcgtgcca gaacgctggc
    32221 tggcaaggcg taacgcttaa taccacgcgg gggcatttct atcgcgcggc gctggaaggg
    32281 ttaaccacgc aattacagcg caatctacag atgctggaaa aaatcgggca ctttaaagcc
    32341 tctgaattat tgttagtcgg cggaggaagt cgcaacacat tgtggaatca gattaaagcc
    32401 aatatgcttg atattccggt aaaagttctc gacgatgccg aaacgaccgt cgcaggagct
    32461 gcgctgtttg gttggtatgg cgtaggggaa tttaacagcc cggaagaagc ccgtgcacag
    32521 attcattatc agtaccgtta tttctacccg caaactgaac ctgaatttat agaggaggtg
    32581 tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa ctattgaaag tgctggcaga
    32641 gatggggcat ggagatgaaa ttattttttc cgatgctcac tttcccgccc attcgatggg
    32701 accgcaggtg atccgcgctg atggcctgtt ggtgagcgac ttgctccagg cgattatccc
    32761 gttatttgag ctggacagtt atgcaccacc gctggtgatg atggcggcgg tagaaggtga
    32821 cactctcgat cctgaagtag aacgacgtta tcgtaatgcg ctttcactac aagccccctg
    32881 tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa aagcctttgc
    32941 gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa aaggggtaac
    33001 accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg atggggtgaa
    33061 aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc ataccagcct
    33121 gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc gtcgcgatct
    33181 caatgaatta cagacgcagg gtaaaattct gcgcaatcat gggcgcgcta aatatatcca
    33241 ccgtcaaaat caagacagtg gcgatccctt tcacatccgg ctgaaaagcc attatgcgca
    33301 taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg tgatagcctt
    33361 agacgccagt tcaacctgct ggtatctggc acgccagttg cctgacatca acatccaggt
    33421 cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca ttcaactgat
    33481 cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct cgctgatttc
    33541 ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga tcgatagcag
    33601 cggcgcactg tgggactcca atgccatcaa cgctgattac aaatcaatgt tattaaaacg
    33661 tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag gggaagcccg
    33721 catcgggcat ctggatgagg taacgcatat tatttctgat gagcgccagg ttacaacttc
    33781 tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg ccttgcctgt
    33841 catcgctcgt cgcgacgacc acccaccgca gcccagatac ggcggacgtg caccgtcact
    33901 tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc atgttcatca
    33961 agctgtgcct gaatatacgc cagattgtgt gacacttctt cgtagcgttt tttcatcggc
    34021 agtttgagat tgaagatagt ttcacggcac cagccattaa ccagccactg cgccattaat
    34081 gccgcaactt tcgccggttt ttcaaccata tcgcatacca tccaggagat attgctgcgt
    34141 gtcggacgga atttgaaacc gtcttcccgc agccacgtca cctgcccggt atccatcaga
    34201 ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt caccagttgg
    34261 taggtccagc cgcctgggca agcacctaaa tccaccgccc acatcccgtt cgccagacgt
    34321 tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt gagcgtggaa
    34381 cgactcggcg catctgccgg aaatttcagg cgcggaatgc ccatatagaa cggcgaatta
    34441 ttgctgctgt atgagtaacc agtatagcaa cagcctggtg caataaagaa tacatgcaca
    34501 accggacgct tcggcgtttc atagttcgcc agcacccccg catcacgcaa ggcagcgcgt
    34561 agcggcacgg tgaatttacg gcagaatttc agcaactctt tgctttcgtt ggtatcggca
    34621 acttcaacac gcagttcacc gcccttctct actacgccct gtaacatgcc aacaatgggg
    34681 gtaatacgat cttctggcgg caaatgctgc aaaagctccc cgactacaaa ccactggcgg
    34741 gcaaaaatta atgaactgaa cggcagctca cggattaact tatcgccatc atcaggttga
    34801 taacactcat aaatgacata acccgcattc tctttcacgc gggcaaaacc gaaaatttcc
    34861 cgctggccgg ctttatcggt aatttctgcg gcgcactctt tttcaaagcc cggacggcac
    34921 agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag caccaactaa
    34981 cattaacgcc cagcccgcaa ggaagctcac gccgccaacc ggtgtgacaa acgcccacaa
    35041 acgcagatgg gacagcgcca ggcaataaag gctgccgctg aacagcaccg tgcctaacgc
    35101 gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg ccaccgccag
    35161 acctaagatc gccagcgtat gaaacgcctg gtattcgagg ccggtctgga tccagcccat
    35221 ctcaacggcc cccatggttt tacttaacac atgcgcgcca aaagcgccca gagctacaaa
    35281 aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg tcgtacccta
    35341 cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg ctttcgccag
    35401 gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat gacaaaccag
    35461 ataaaaagcg tttttactga ccagaacatc attaaacggg caaacaagac gtccggcctc
    35521 gatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt ggatagccgc
    35581 ttgcagcacc atggcgctat ggctaaaaat tggcccttgc tgaacgttga tatgatttaa
    35641 ccccaactgt cgggtatatg tctgccagtc acggcgcgac gcatcatgta ataacgtatg
    35701 tttagccaga tcttccgggg tcttcaaggg tttttcgcca gtcagcagta gcggcgaaca
    35761 caccggcaat aaatattcgg cgtacagttt ttccacccgt agccccggcc agttgccccg
    35821 accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac gatcaaccgc
    35881 ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc gcggaaccaa
    35941 ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac gcccccttag cgctacgggc
    36001 ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat cgaggaaata
    36061 gctttgacct tcctcggtca gcaggagtga acgattacgg cggcggaaca gttttagccc
    36121 cagaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca caaaaagctc
    36181 ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc gtaaggcatt
    36241 tagcggtggt aatcgtttag acatggcttt taaactttga tgttaaatga attt
//
LOCUS       DAZKHK010000040        36173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKHK010000040 DAZKHK010000000
VERSION     DAZKHK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.040"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..755)
                     /gene="aqpZ"
                     /locus_tag="ACHOS5_001985"
     CDS             complement(60..755)
                     /gene="aqpZ"
                     /locus_tag="ACHOS5_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP9776803.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1181..2839
                     /gene="ybjD"
                     /locus_tag="ACHOS5_001986"
     CDS             1181..2839
                     /gene="ybjD"
                     /locus_tag="ACHOS5_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9776804.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2836..3792)
                     /gene="ybjX"
                     /locus_tag="ACHOS5_001987"
     CDS             complement(2836..3792)
                     /gene="ybjX"
                     /locus_tag="ACHOS5_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP9776805.1"
                     /translation="MNMSQLTERTFTPSESLNSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            3943..5058
                     /gene="macA"
                     /locus_tag="ACHOS5_001988"
     CDS             3943..5058
                     /gene="macA"
                     /locus_tag="ACHOS5_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP9776806.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5055..7001
                     /gene="macB"
                     /locus_tag="ACHOS5_001989"
     CDS             5055..7001
                     /gene="macB"
                     /locus_tag="ACHOS5_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP9776807.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPDWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7074..7298)
                     /gene="cspD"
                     /locus_tag="ACHOS5_001990"
     CDS             complement(7074..7298)
                     /gene="cspD"
                     /locus_tag="ACHOS5_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP9776808.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7448..7522
                     /gene="yljB"
                     /locus_tag="ACHOS5_001991"
     CDS             7448..7522
                     /gene="yljB"
                     /locus_tag="ACHOS5_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP9776809.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7621..7941
                     /gene="clpS"
                     /locus_tag="ACHOS5_001992"
     CDS             7621..7941
                     /gene="clpS"
                     /locus_tag="ACHOS5_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP9776810.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7972..10248
                     /gene="clpA"
                     /locus_tag="ACHOS5_001993"
     CDS             7972..10248
                     /gene="clpA"
                     /locus_tag="ACHOS5_001993"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP9776811.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10591..10678)
                     /locus_tag="ACHOS5_001994"
     tRNA            complement(10591..10678)
                     /locus_tag="ACHOS5_001994"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10642..10644),aa:Ser,seq:gga)
     gene            complement(10932..11150)
                     /gene="infA"
                     /locus_tag="ACHOS5_001995"
     CDS             complement(10932..11150)
                     /gene="infA"
                     /locus_tag="ACHOS5_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9776812.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11435..12139)
                     /gene="aat"
                     /locus_tag="ACHOS5_001996"
     CDS             complement(11435..12139)
                     /gene="aat"
                     /locus_tag="ACHOS5_001996"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP9776813.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12181..13902)
                     /gene="cydC"
                     /locus_tag="ACHOS5_001997"
     CDS             complement(12181..13902)
                     /gene="cydC"
                     /locus_tag="ACHOS5_001997"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP9776814.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGT
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13903..15669)
                     /gene="cydD"
                     /locus_tag="ACHOS5_001998"
     CDS             complement(13903..15669)
                     /gene="cydD"
                     /locus_tag="ACHOS5_001998"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP9776815.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15792..16757)
                     /gene="trxB"
                     /locus_tag="ACHOS5_001999"
     CDS             complement(15792..16757)
                     /gene="trxB"
                     /locus_tag="ACHOS5_001999"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9776816.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17301..17795
                     /gene="lrp"
                     /locus_tag="ACHOS5_002000"
     CDS             17301..17795
                     /gene="lrp"
                     /locus_tag="ACHOS5_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP9776817.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17930..22036
                     /gene="ftsK"
                     /locus_tag="ACHOS5_002001"
     CDS             17930..22036
                     /gene="ftsK"
                     /locus_tag="ACHOS5_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9776818.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPHQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPTRPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQPHD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGVGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22195..22806
                     /gene="lolA"
                     /locus_tag="ACHOS5_002002"
     CDS             22195..22806
                     /gene="lolA"
                     /locus_tag="ACHOS5_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP9776819.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22817..24160
                     /gene="rarA"
                     /locus_tag="ACHOS5_002003"
                     /gene_synonym="mgsA"
     CDS             22817..24160
                     /gene="rarA"
                     /locus_tag="ACHOS5_002003"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP9776820.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24251..25543
                     /gene="serS"
                     /locus_tag="ACHOS5_002004"
     CDS             24251..25543
                     /gene="serS"
                     /locus_tag="ACHOS5_002004"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9776821.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25782..28226
                     /gene="dmsA"
                     /locus_tag="ACHOS5_002005"
     CDS             25782..28226
                     /gene="dmsA"
                     /locus_tag="ACHOS5_002005"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP9776822.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATKMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGISNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28237..28854
                     /gene="dmsB"
                     /locus_tag="ACHOS5_002006"
     CDS             28237..28854
                     /gene="dmsB"
                     /locus_tag="ACHOS5_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP9776823.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28856..29719
                     /gene="dmsC"
                     /locus_tag="ACHOS5_002007"
     CDS             28856..29719
                     /gene="dmsC"
                     /locus_tag="ACHOS5_002007"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP9776824.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWGLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELANIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29755..30381)
                     /gene="ycaC"
                     /locus_tag="ACHOS5_002008"
     CDS             complement(29755..30381)
                     /gene="ycaC"
                     /locus_tag="ACHOS5_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP9776825.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30695..31843
                     /gene="ycaD"
                     /locus_tag="ACHOS5_002009"
     CDS             30695..31843
                     /gene="ycaD"
                     /locus_tag="ACHOS5_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776826.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTALTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(31940..32680)
                     /gene="pflA"
                     /locus_tag="ACHOS5_002010"
     CDS             complement(31940..32680)
                     /gene="pflA"
                     /locus_tag="ACHOS5_002010"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP9776827.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(32872..35154)
                     /gene="pflB"
                     /locus_tag="ACHOS5_002011"
     CDS             complement(32872..35154)
                     /gene="pflB"
                     /locus_tag="ACHOS5_002011"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9776828.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(35209..36066)
                     /gene="focA"
                     /locus_tag="ACHOS5_002012"
     CDS             complement(35209..36066)
                     /gene="focA"
                     /locus_tag="ACHOS5_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP9776829.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         8988 a         8893 c         9627 g         8665 t
ORIGIN      
        1 tcaacatata acaacatctt aaaaaaaggc ctgacattgc gccaggcctt ctgcgttaat
       61 taattacgct tttccagtag agtccggtaa atcagaccac cgataatacc gccgacaatt
      121 ggcaccaccc agaagaacca cagttgctct aatgcccagc cgccctggaa tatagcaacc
      181 gcagtactgc gcgccgggtt aacggaagtg ttagtcaccg gaatactgat taagtgaatg
      241 agggttaagg ccagaccaat ggcaattggc gcaaaaccag caggagcgaa tttgtcagtt
      301 gcgccgtgga tcaccagcag gaaacctgca ctcaatacca gttcaactac cagcgcggaa
      361 agcatggaat aaccgcctgg tgaatgctcg ccataaccgt tagaagcaaa accgctggct
      421 gccgcgtcaa aacccgtttt accactggca attaaataca gcagcgccgc cgcaacaata
      481 ccgccgacaa cctgggcaat tacatagcca acgacttctt ttgccgggaa acgtccgcca
      541 gcccataaac caatagtgac cgccgggtta aaatgaccac cagaaatatg accaacagca
      601 aaggccattg tcagaacggt cagaccgaac gccaacgcca cgccggcaaa accaatgcct
      661 aattccggga agcctgcggc cagtacggca ctaccacagc cgccaaaaac aagccagaaa
      721 gtaccaaaac attcagctgc taattttctg aacatatcca cctcaattaa aaattgaccc
      781 tgtgaaaaat atggtcgttt tatagggtcg tcgtaaaaaa gtgacgacgg aaatgatgcg
      841 cggctatttt aaaaacgaag gcaagtcatt caccagataa ataaatccag taaatttgat
      901 ttaaggcaac agcgggttgc cccatatagt aatttgtctg attgacagtg tagtgcaagc
      961 gaaagattta atcctttagg cgtaataaaa aataatttat catgctaatt atttgatttt
     1021 gttgtttttg caggcttatc agcaagaggg agtataacgc gattattcgc tcatttttca
     1081 gacatttgcc atgcttaaat gtgatgtcat cacgtattag catggccttt cccgttatac
     1141 tgccagcgta aaggataagt cacatatttc tggaggggat atgattcttg agcgcgttga
     1201 aattgtgggt tttcgcggta tcaaccgttt gtcgttgatg ctggaacaaa acaacgtcct
     1261 gattggggag aacgcgtggg gtaaatccag cttgctggac gccttaaccc tgctgctatc
     1321 gccagaatca gatctctacc attttgagcg cgatgatttc tggttcccgc cgggagatat
     1381 caacgggcga gaacatcatc tgcatattat tttgaccttc cgcgaatcgc tgccaggccg
     1441 acatcgggtt cgccgctatc ggccgctgga agcgtgctgg acgccatgca ccgatggtta
     1501 tcaccgtatt ttttatcgtc tggaagggga gagtgcggaa gacggcagcg tgatgacact
     1561 ccgcagtttt ctcgataaag acggacatcc gattgatgtt gaggatatta acgatcaggc
     1621 tcgtcatctg gtgcgtttaa tgccggtgct gcgcttgcgt gatgcccgtt ttatgcgccg
     1681 tattcgtaac ggcacggtgc caaatgtccc caatgtggaa gtcaccgcgc gccagctcga
     1741 tttcctcgcc cgtgaattat cctcacatcc gcaaaatctc tctgatgggc agattcgtca
     1801 gggactttcc gcaatggtac agctgcttga gcattatttc tctgagcagg gggccggaca
     1861 ggcgcgatat cgtttaatgc ggcggcgagc cagcaatgag caacgaagct ggcgctatct
     1921 ggatatcatc aaccggatga ttgaccgacc tggagggcgc tcgtatcggg ttattttgct
     1981 cggcctgttt gctactttat tgcaggcaaa aggcacattg cgactggata aagacgcccg
     2041 tccattgttg ctgatcgaag atccagaaac ccgtttgcac cccattatgc tttcagttgc
     2101 ctggcatctg ttgaaccttc tgccattgca gcgcatcgct actaccaact cgggagagtt
     2161 gctttcgtta acgccggtag agcatgtttg ccgactggta cgtgagtcct cgcgcgttgc
     2221 cgcctggcgt ctggggccga gtggtttgag taccgaagat agccgacgca tctcttttca
     2281 cattcgtttt aatcgtccgt catcgctgtt tgctcgctgc tggttgctgg tggaagggga
     2341 aacagaaacc tgggttatca acgaactggc gcgccagtgt ggacaccatt tcgatgccga
     2401 agggattaag gtcattgaat ttgcccagtc cgggctaaaa ccactggtta aatttgcccg
     2461 gcgaatgggg attgaatggc atgtactggt cgatggcgat gaagcaggga agaaatatgc
     2521 cgctacggta cgcagcctgt tgaataacga tcgggaagcc gaacgagaac atttaacggc
     2581 gttaccggcg ctggatatgg aacattttat gtatcgccag ggattttccg atgtgttcca
     2641 ccgcgtggcg caaatcccgg aaaatatacc gatgaatctg cgcaaagtta tctcgaaagc
     2701 gatccatcgc tcttccaaac ccgatcttgc cattgaagtg gcaatggagg ccggacgtcg
     2761 tggtgtggat tccgtaccga ctctgctgaa aaaaatgttc tcacgcgtgc tgtggctggc
     2821 gcgcggtcgg gccgactaac tacaaaacat cgtggccatt tgtggctgaa tagcgtcgag
     2881 catctcatag cgccgacggt attcagcccg ttttttactg gcgatttcgg caatctcttt
     2941 tcgtgctatc tgtgctggaa ggcggtaatg gcgttcagca tcacatacgc cgccaaccga
     3001 ttcccagaaa gcgttgtaat cggcatggat cttgccttct ttatcgcgat aacgcaggct
     3061 gcggtaaata tgcgtttcat tgctgacggc aataatctgc tctacctgca aacgttgggc
     3121 aaacagacag gccgcttcca tcacgaggcg tttgggaaat agcccgtggc aggctttcgt
     3181 cgcattctgg atttcctggt gtggaatttc ccattttgcg ccttgcagtc cgccaataaa
     3241 catcgtcctt ttcccctgat attcacacag ggtaaaagtg agctctgcca gaggaatacc
     3301 ttcgctgttg cggaacagga tggtgctgtc accttcttta tccattgaga tcatcatggt
     3361 cagctcaagc gtgaactgct cgccgttttt gccttccagc ttcgccagtt gcagcccggg
     3421 ggtattcaaa tataagctga attcttccgc cgacatacac ccgcggagta acgcataatg
     3481 gtaacgtaac gcctccagca attgcttacg gctaagattc gccgcaaggt aagggcgatg
     3541 tagacgtaca ggcagtcgcg gctggcgcgt taacaacaca ttgagattag gccagtggga
     3601 aagttcgttc atccactcaa cgcttaaacg cggcataatc aacgagcgca gcaaaaattt
     3661 ctggcgaaaa ctacggcgat gccagaattt acccggccga cactgtcctc gtgccagact
     3721 aagaaaaagt gacaggctgt tgagagattc agatggcgta aaggtccgtt cagttagctg
     3781 cgacatattc atgaaatcaa tggttataca tgacgtcgat ttaaccattg cgtatcttaa
     3841 ccaaacatca atagtgtgat tattaacgta aattttaggg ttttgttgat atttcgttga
     3901 agttaatgac ccggattggc atatggagta ttcagaatat ttatgaaaaa gcggaaaacc
     3961 gtgaagaagc gttacgttat tgcgatggtg atagtcatcg ccgggctgat tacgttatgg
     4021 agaattctta acgcacccgt gccgacttat cagacgctga ttgtgcgccc cggtgattta
     4081 cagcaaagcg tgctggcgac cggaaagctg gacgcgctgc gtaaggttga cgtgggcgcg
     4141 caggtcagcg gtcagttgaa aacgctgtcg gtggcgattg gcgataaagt aaaaaaagac
     4201 cagcttttag gggttattga tcctgaacag gctgaaaacc agatcaagga ggtggaagca
     4261 acgctgatgg agctacgcgc gcagcggcag caggcggaag cggagctgaa actggcgcgg
     4321 gtgacgtatt cccgtcagca acgtcttgca caaacgcaag cggtttcaca gcaggatctc
     4381 gataacgccg cgacggagat ggctgtgaaa caggcgcaaa ttggcaccat tgacgcgcaa
     4441 atcaagcgca atcaggcctc tctcgatacg gctaaaacca atctcgatta cacccgcatc
     4501 gttgccccga tggccgggga agtcacgcaa atcaccactc tgcaaggcca gacggtgatt
     4561 gccgcacaac aagcaccgaa cattctgacg ctggcagata tgagcaccat gctggtaaaa
     4621 gcgcaggttt ctgaagcgga tgtaatccac ctgaagccgg ggcaaaaagc ctggtttacg
     4681 gtgcttggcg atccactgac gcgctacgag gggcaaatca aggatgtgct accgacgccg
     4741 gaaaagatta acgacgctat tttctattac gcccgttttg aagtacccaa ccccaatggt
     4801 ttactgcggc tggatatgac cgcgcaagtg catattcagc tcaccgatgt gaaaaatgtg
     4861 ctgacgatcc ctctgtcggc gttaggcgat ccggttggcg ataatcgtta taaagtcaaa
     4921 ttgttgcgta atggtgaaac acgcgagcgt gaagtgacga ttggcgcacg taacgatacc
     4981 gatgttgaga ttgtcaaagg gctggaagcg ggcgatgaag tggtgattgg tgaggccaaa
     5041 ccaggagctg cgcaatgacg cccttgctcg aattaaagga tattcgtcgc agctatcctg
     5101 ccggtgatga gcaggttgag gtgctgaagg gcatcagcct cgatatttat gccggtgaga
     5161 tggtagcgat tgtcggcgct tcgggttctg gtaaatcgac cctgatgaat attctcggct
     5221 gcctggataa ggccaccagc ggcacctatc gcgtcgccgg tcaggatgtt gccacgctgg
     5281 acgccgatgc gctggcgcaa ctgcgccgcg agcatttcgg ctttattttc cagcgttacc
     5341 atttgctttc gcatttaacc gccgagcaaa acgttgaagt acccgccgtc tatgccggtc
     5401 ttgagcggaa acagcggctg cttcgcgccc aggagttact gcaacgactg gggctggaag
     5461 accgtacaga gtattacccg gcacagcttt cgggtggcca gcaacagcgc gtcagcatcg
     5521 cgcgggcatt gatgaacggc ggtcaggtaa ttcttgccga tgaaccgacc ggcgcactgg
     5581 acagccattc tggcgaagag gtgatggcga tcttgcatca gctgcgcgat cgcgggcata
     5641 cggtgattat cgtcacacac gatccgcagg ttgctgctca ggccgagcgg gtgatcgaaa
     5701 ttcgcgacgg cgaaattgtg cgcaatcctc ccgccgttga aaaagtgaat gctactggcg
     5761 gaacggaacc tgttgtcaac acggcgtctg gctggcggca gtttgtcagc ggttttaacg
     5821 aggcgctgac gatggcatgg cgggcgctgg cagcgaataa aatgcgtact ttactgacca
     5881 tgctggggat tattatcggt attgcgtcgg tggtttccat tgtcgtggtg ggcgacgccg
     5941 ccaaacaaat ggtgctggcg gatattcgtt ctattggtac gaatactatt gatgtctatc
     6001 ccgggaaaga ttttggcgat gacgatccgc aatatcagca ggcgctgaag tacgacgact
     6061 taatcgccat ccaaaaacaa ccgtgggttg cctctgccac gcccgccgtc tcgcaaaacc
     6121 tgcgcctgcg ttataacaat gttgatgtgg ctgccagtgc caatggcgtg agcggcgatt
     6181 attttaatgt ctatggcatg accttcagtg aagggaacac ctttaatcag gagcagctga
     6241 acggtcgtgc gcaggtcgtg gttctcgaca gtaatactcg ccgccagctt ttcccccata
     6301 aagcagatgt ggttggcgag gtgattctgg tcggcaatat gcccgccaga gtcattggtg
     6361 tggcggaaga aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc tggctgccat
     6421 acagcacgat gtccgggcga gttatggggc agtcgtggct gaactccatt actgtcaggg
     6481 tgaaagaagg ctttgacagc gctgaggcgg aacagcaact cacgcgttta ctttcactgc
     6541 gccacggtaa gaaggatttc tttacctgga acatggatgg cgtcttgaaa accgttgaaa
     6601 agaccacacg tactttacaa ctgtttctga cgctggttgc ggtgatttcg ctggtggtgg
     6661 gcggtattgg tgtaatgaat attatgctgg tgtcagtgac cgagcggacg cgggaaattg
     6721 gcattcgcat ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttt ttgatcgaag
     6781 ccgtactggt ttgcctggtt ggtggcgcgt tgggaataac actgtcactg ttaattgctt
     6841 tcaccttgca gcttttctta cccgactggg agattggctt ttcaccgttg gcactgctgc
     6901 tggcgtttct ctgctcgacg gtcaccggga ttttatttgg ctggttaccc gcacgaaatg
     6961 cggcacgact ggatccagta gatgctctgg cacgagagta atttttgaga taaaaatgcc
     7021 agccgatcgg gctggcattt tgcctttagg atgtacacaa tgagacagaa gagctatgcg
     7081 actgccgctt ctacttcgac gggcacaata acactggcgt gattgccttt tggcccctgg
     7141 tggacatcaa actgaacgga ttgtccagct tttagcgttc tgtaaccatc catctgaatg
     7201 gtggaataat gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa accaaaccct
     7261 ttggcattgt tgaaccactt gacagtaccc ttttccatgc ttcgacatcc ttcgcaaatc
     7321 ttatacaagt aagatggaat aaaccggggt cagagagggg gctgttcaaa acctcgccaa
     7381 ctctagaaat acaatttaga gaattagggc gagccgtcaa gcatttgaca ggggccaggg
     7441 ggcaggtatg aatcaaaaat ttgaagcagt taacgctatt gacaggaatg tgacagatgt
     7501 cgctgatgcc aacgatagat gatagttatc tatcatgtgg agtagattgg tcaggcaaat
     7561 aagctcttgt cagcggcagg gcgttctgcc gataaccgta accgaagatg ataactgaca
     7621 atgggtaaaa cgaacgactg gctggacttt gatcaactgg cggaagaaaa agttcgcgac
     7681 gcgctaaaac cgccatctat gtataaagtg atattagtca atgatgatta tactccgatg
     7741 gagtttgtta ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg tgcaacgcaa
     7801 ttgatgctcg ctgttcacta ccaggggaag gccatttgcg gagtctttac cgctgaggtt
     7861 gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg agaatgagca tccattgctg
     7921 tgtacgctag aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc tatgctcaat
     7981 caagaactgg aactcagttt aaatatggct ttcgccagag cgcgcgagca ccgtcatgag
     8041 tttatgaccg tcgagcactt gttactggcg ctgctcagta acccatctgc ccgggaggcg
     8101 ctggaagcgt gctctgtgga tttggttgcg ctccgtcagg aactggaagc ctttattgaa
     8161 caaaccacac ccgttctgcc tgccagtgaa gaggagcgtg acacacagcc gacgctgagt
     8221 tttcagcgag tactgcaacg tgcggtcttc catgtccagt cctccggtcg caatgaggtt
     8281 accggtgcaa acgttctggt cgctatcttt agcgaacagg agtcgcaggc ggcatatctg
     8341 ttgcgcaaac atgaagtcag ccgtctcgat gtggtgaact ttatctctca tggcacgcgt
     8401 aaagacgagc cgacacagtc ttctgatcct ggcagccagc caaacagcga agaacaagct
     8461 ggtggggagg aacgtatgga gaatttcacg acgaacctga atcagcttgc gcgagtgggc
     8521 ggaatcgacc cactgattgg tcgtgagaag gagctggagc gtgctattca ggttctctgc
     8581 cgtcgccgta aaaacaaccc gctgctggta ggggagtctg gtgtcggtaa aaccgcgatt
     8641 gcggaggggc ttgcatggcg aattgttcag ggcgatgtgc cggaagtgat ggctgactgt
     8701 acgatttact ctctcgatat cggttctctg ttagcgggca caaaatatcg cggcgacttt
     8761 gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcaag acactaacag catcctgttt
     8821 attgatgaga tccacaccat tatcggtgcg ggtgcagcgt ctggtggcca ggtcgatgcg
     8881 gctaacctga tcaaaccgtt gctctccagc ggtaaaatcc gcgtaattgg ttcgacaacc
     8941 tatcaggagt tcagcaacat ttttgagaaa gaccgtgctc tggcgcgtcg cttccagaaa
     9001 attgatatta ctgaaccgtc aatcgaagaa actgttcaaa tcatcaatgg cctgaaaccg
     9061 aagtatgaag cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc ggcggtagag
     9121 ctggcggtga aatacattaa cgatcgtcat ctgccggata aagcgattga tgttatcgac
     9181 gaagcgggcg ctcgcgcacg tctgatgccg gtaagcaaac gcaagaaaac cgttaatgtg
     9241 gcggatattg aatccgtggt ggcccgtatt gcacgcattc cagagaagag tgtttctcag
     9301 agtgaccgcg ataccctgaa aaacctcggc gatcgcctga aaatgctggt cttcggtcag
     9361 gataaagcca tcgaggcgct gactgaagcc attaagatgg cgcgtgcagg tttaggtcac
     9421 gaacataaac cggtcggttc gttcctgttt gccggcccta ctggggtcgg gaaaacagag
     9481 gtgacggtac agctttcgaa agcgttgggc attgagcttc tgcgctttga tatgtccgag
     9541 tatatggaac gccataccgt cagccgtctg attggtgcgc ctccgggata cgttggtttt
     9601 gatcagggcg gtttgctgac tgatgcggtc atcaagcatc cacatgcggt gctgctgctg
     9661 gacgaaatcg agaaagcgca cccggacgtg ttcaacatcc tcttgcaggt gatggacaac
     9721 ggtacactga ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt gctggtgatg
     9781 accactaacg cgggggtacg tgaaaccgag cgcaaatcca ttggtcttat ccaccaggat
     9841 aacagcaccg atgcgatgga agagatcaag aagatcttta caccggaatt ccgtaaccgt
     9901 ctcgacaaca ttatctggtt cgatcatctg tcaaccgacg tgatccatca ggtggtggat
     9961 aaattcatcg tcgagttgca ggttcagttg gatcagaaag gtgtttctct ggaagtgagc
    10021 caggaagcgc gtaactggct ggccgagaaa ggttacgacc gggcaatggg cgcacgtccg
    10081 atggcgcgtg tcatccagga caacctgaaa aaaccgctcg ccaacgaact gctgtttggt
    10141 tcgctggtgg acggcggtca ggtcaccgtc gcgctggata aagagaaaaa tgagctgact
    10201 tacggattcc agagtgcaca aaagcacaag gcggaagcag cgcattaatc tgattgtcag
    10261 gtaggttggt taagtccgtg atctcgtcag gggttacgga ctttttattt atggggggag
    10321 gaggttcaga cccttttttt aatgatgatg ctaagttatt gataattagt gctgcgggta
    10381 ggtaaggata aaaaagggtg gcagcaggag attgagatgg ttttgcttta ttaacaacgg
    10441 gctaaacgtg tagtatttga gtttactgcc gtacaggcag cttagaaatt cactggtaac
    10501 atactccacc cgcccaccat gttcactgcc gtacaggcag ataaaatgcg aaaaaaagcc
    10561 cgtactttcg tacgagctct tctttaaaga tggcggtgag ggggggattc gaacccccga
    10621 tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa
    10681 attgttttgt tgcctgacct catggggggc aacggggcgc tactataggg agttggaata
    10741 aaacggtcaa gaagaatttt aatgataatt attgtttgct catactgtaa acaagttgtg
    10801 cagtatatct acatcgagac aagttacgga cttatacttc taaagtactc catacatatc
    10861 acaaaataaa aaggccggct aaaccgacct tttactcatt ctttctcttc gcccatcagg
    10921 cggtaaaaca atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc
    10981 agttcaacag tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg
    11041 gagatgtgtg cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc
    11101 aacgtttcaa gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct
    11161 ggggtatcac taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa
    11221 agatttgcgc gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct
    11281 aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg
    11341 acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta accaactctg cgaccgcaat
    11401 tataacactc tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa aacaagcatc
    11461 gaggtaccca gaaattattc ggcaatcgtc cgaggcgcat ttgattgaga taattaaggt
    11521 aatcccggcg gggaatttcg caggcaccaa gcgatgctgt gtgatcgtta aggacctggc
    11581 agtcgataag tttaccgcca tgacggataa attcatcaca gaagaccaga agcgccgttt
    11641 tggacgcatt ttccatccgg ctgaacatgg actcgccaca aaatagtgtt ccctgggcca
    11701 cgccgtacat accgccgaca agctcatctt cacgccagac ttcaatggag tgggcatgac
    11761 cgagttcgtg aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt
    11821 cgcgatcgct ggcacagcct tcgatgacct gaccaaaagc gtaattcatc gtgacacgat
    11881 agggtgagcg tttatgaaat cgcttcatac tacggctgat atgcagtgat tctggccata
    11941 acaccgcgcg gggatcgggc gaccaccaga ggatggggtc gccaggagaa aaccacggga
    12001 aaataccacg ctgataagcc attaacaggc gcgcagggct aagatcgccc cccagtgcca
    12061 gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag
    12121 aaagctgaac caggcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc
    12181 ttacaaaccc tgcttaaact ggtaataacg cccctgtctg gcaagcagtt ctgcgtgagt
    12241 accttgctca ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc
    12301 gcgaagtcga tgggtgacca ttaacaccgt tttctcgcac atcatttctg caagcaattc
    12361 aaggatctga ctttcggttg tggcatctaa gccttctgta ggttcatcca gcaataccag
    12421 cggcgcatca tgtaacagcg cacgggcgat agccagacgg cgcagttcac caccggatag
    12481 ctggcgtccg ccttcaccta accaactgtt gagacctgca tcctcgagca gcttctccag
    12541 accaacgcga cgcaagatct ccgccagagc ctcatcacta ctaccaggcg aggcgagtaa
    12601 cagattatca cgcagcgtgg cgctaaacag atgcactcgc tggggaacaa cgctgatggt
    12661 ctgtcgtaga gccgcttcat tcaggctggc tatgggacta tcgttaagca aaatctcgcc
    12721 ctgttgcggg tcccatgcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt
    12781 tcgcccgaga atcgcaatat gttccccggc gtttacctga agataaatcc ctttaagtgc
    12841 ctgttgggat tgctccggat aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc
    12901 ggcaacacga gtttgggtat cagggaaggt gacttccggt ttttgatccg ttaaatcagt
    12961 gatccgtacg gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc
    13021 cagcgcttca aacgcggcta acgcgcagaa gacaaacagg gcaattaacg cgccgggttg
    13081 agcattgccg ccaacgccgc cggacgccat ccacagcatc aggatcaccg ctaacgcgcc
    13141 aatgagcagc attatcgctt gcgacaatgc ggtcagttca gattgacggc gttgcgcttc
    13201 cagccactga atttctgtat tctctaattg cgtgcgataa cgatcgctgg taccaaaaat
    13261 ggtcagctca gcttgccctt gcagccaggc cgtcagttgt tggcgatact gcccgcgaag
    13321 atgagtcaga ttttgcccgg tgctttttcc cgcacgataa aacagtggtg gcatcaggaa
    13381 aagcgtcagt aacataatgc cgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa
    13441 cccgattgtc accaccataa tcaccacaaa agcacctacc agcggcgaga taacgcgcag
    13501 gtaaagatga tcgagcgtat caacatccgc caccacgcgg ttgagtaatt cgccctgacg
    13561 atagcgcgcc agtccggcag gggagagggg cagcaatttg ctgaaggtgt aaatgcgcag
    13621 atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat aacgcccggc
    13681 agtacgggtg attgctgcgc cacgcacgcc cgcagcgggt agcatatagt tgaagctgta
    13741 cagtccggca acccccgcta ccgctgaggc ggagaggaac cagccggaaa gggtcaacag
    13801 accgatactg gcgagcagcg tgacaattgc cagcacgata ccaagactta acatccattt
    13861 atgacgttta tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg
    13921 atgggccagt aatgtggcga atgggccgcc agccacactt aattccgcgt aacgtccttg
    13981 ctcaataatc tgaccatcct gcattaccca aatgacatcc cagtcagcaa gatcttctaa
    14041 ctggtgggtg accattaacg ttgtctggcg cagagaggcg gcattcagcg cctccattac
    14101 gcgctgttca ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca
    14161 gggatttagt aacgcacggg ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc
    14221 agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg agcggtagaa actcactgac
    14281 ccaggcgtta tccagcgctg tttgtaactc ttgttcgctg gcatcaggtc gcgccagtag
    14341 tacgttatcc cgtaatgttg ctgccggtaa ttgtgggttt tgcccaaccc aggagagatg
    14401 tttacgccat gattccggtg ataaatcgcg taattctatc ccgttgattc gtagcgatcc
    14461 ctgatatgag agaaaaccag aaagcgcgtt caacagtgaa cttttgcctg aaccgctgcg
    14521 accaaccaac actgctcgtt ggcctgctgg caaagtaaaa ttcagcggtc cggccagcgt
    14581 tttaccttcc ggcgacgtga taaacagatc ctcggcttca atggtcaccg gatcggtcga
    14641 tgctaattcc gcttcaccgc gctgcggatg ggctagcggg gtttccataa acgttttcaa
    14701 actgtcagct gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgtaa
    14761 tggctggaaa aactctggcg caaggatcag ggccagaaaa cccgcagcca gcgtcacgcc
    14821 agtatcgtag tgaccaaaat ccagctcgcc gaggtaggaa aaaccaaagt agaccgccac
    14881 cagagcaatc gacagcgagg taaaaaattc gagaatgccg gaggataaaa acgccagccg
    14941 tagcacttcc attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc
    15001 agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg
    15061 cccacttaag cgagcaagag cgagaaagtt acgtcggtta gcatcggcag cccccattcc
    15121 aaccagtgcc ataaataacg gaattagcgg tgcagtgccc agcagaatga gcgccgcagc
    15181 ccagttagag gggaagatag ccaccacaat cagcagcggc accgacactg ccagcgccat
    15241 ttgcggcagg tagcgtgcat agtaatcatg catatcgtca atttgctcga gcaccagcgt
    15301 cgcccagctc cccgcaggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc
    15361 gagaacctga cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc
    15421 gcgtaaccag accacccatg cgcgcagtac aaaggtcaga aacagtaacg taaagggaag
    15481 cagcagggct tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa
    15541 ccaggcctgg gcaatgatca atatgccgct cacaaagccc agcagacgag aaatattcag
    15601 ccaacgttgg gagatgacgc tttgctgttt taaccagcgg gttaattctt tttgacggga
    15661 tttattcatt gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa
    15721 cggggaaaaa ataaaggcga cccatagtcg catggtgtcg ccttctttac ttttgttact
    15781 gatttgtaaa attattttgc gtcagctaaa ccgtcgaggt agcgttccgc atcaagagct
    15841 gccatgcagc ctgtaccagc agatgtaatg gcctggcgat aaatgtgatc catcacgtcg
    15901 cctgcggcaa agacgccagg gatgctggtc tgggtggcat taccatgaat acccgactgt
    15961 actttgatgt agccgttttc cagttccaac tgcccttcga aaatggcagt attcgggctg
    16021 tgaccgatag caacaaacag accggcaacg tcgagtgact cgatgttatc gctgttttgc
    16081 gtatcgcgca gacgaacgcc agtgacaccc atctgatcgc cggtcacttc ttccagcgta
    16141 cggttggtgt gcagaataat gttgccgttc tccactttat ccatcagacg cttaatgagg
    16201 attttttccg cacggaaacc atctcggcgg tgaatcagat gaacttccga agcgatgtta
    16261 gacagataca gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg
    16321 ttgcgataga agaaaccgtc gcaggttgca caagcagaaa ctccacggcc tttaaaggct
    16381 tcttcagagg gcaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg
    16441 caagtgtatt cgccgctatc gccagtcaga cggaacggac gattctgcaa atcaactttg
    16501 ttgatgtgat caaagatgat ttcagtttca aatttggtgg catgttcgtg catgcgctcc
    16561 attaataacg gaccggtcag atcgtttgga tcgccaggcc agttttccac ttccgtggtg
    16621 gtggtcagtt ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc
    16681 gccgcgtaga cagcagcggt gtatcccgcc gggcctgaac ccaggataag cagtttactg
    16741 tgtttggtcg tgcccatgag atccccatag ttgttggcag acaatggcag gattgtaggg
    16801 aatttacaga cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc
    16861 atcgataagc aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt
    16921 tataagccga ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact
    16981 aaaaatcgat gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa
    17041 aaaaaacagt attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta
    17101 gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc
    17161 aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct
    17221 atcgtgacgg gtagcgactc tgaacagtga tgtttcaggg tcagacagga gtagggaagg
    17281 aatacagaga gacaataata atggtagata gcaagaagcg ccctggcaaa gatctcgacc
    17341 gtatcgatcg taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc
    17401 tttctaaacg tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa
    17461 gacaagggtt tattcagggc tatacggcgc tgctgaaccc ccattatctg gatgcatcac
    17521 ttctggtatt cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca
    17581 ataccgctgt acaaaaactt gaagaaattc aggagtgtca tttagtatcc ggtgatttcg
    17641 actacctgtt gaaaacacgc gtgccggata tgtctgctta ccgtaagtta ctgggggaaa
    17701 ccctgctgcg tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca
    17761 agcagagtaa tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt
    17821 attttgatta cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt
    17881 gtccgtttta gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga
    17941 atacactgaa gacaaagaag tcacattgac aaagttaagt agcggccgcc gtcttctgga
    18001 ggcgttgctg atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt
    18061 taacccttcg gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg
    18121 tgggatgccc ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta
    18181 caccattccc gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga
    18241 cgaatacatt gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct
    18301 tacctcctgt ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg
    18361 cgtcattggc agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac
    18421 tattgcgctg ctctgcgttt gggcagcggg cctgacgctg ttcaccggtt ggtcatgggt
    18481 gaccattgct gaaaaactcg gcggctggat tttaaatatt cttaccttcg ccagtaatcg
    18541 tacccgtcgc gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga
    18601 agatgaaaat cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct
    18661 agcgcgtcgt aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagatgc
    18721 tgcgctgttc tccggtaagc ggatggatga tgaagaagag attacctaca ctgcacgcgg
    18781 tgtggctgct gaccctgacg acgtcctgtt ttcgggcaat cgtgcaacgc agccagaata
    18841 tgacgaatac gatccattat taaacggtgc gccaattacc gaacctgttg ctgtagcagc
    18901 tgctgctacc acggcgacac aaagctgggc tgcaccggtt gaacctgtga ctcagacgcc
    18961 gccagttgcc tctgttgatg ttccacctac gcaacctacg gtagcctggc agcctgtacc
    19021 ggggccgcaa acgggagaac cggttattgc acctgcaccg gaaggttacc cacaccagtc
    19081 acaatatgcg cagcctgcag tgcaatataa tgagccgctg caacaacctg tacagccaca
    19141 gcagccgtat tatgcgcctg cggctgaaca acctgtgcaa caaccttact acgctcctgc
    19201 ggcggaacaa cctgttcagc aaccgtatta cgctcctgcg gcggaacaac ctgttcagca
    19261 accgtattac gcccctgcgc cagaacaacc ggtggcaggt aacgcctggc aagccgaaga
    19321 gcagcaatcc acttttgctc cacagtctac ataccagacg gagcaaactt atcagcagcc
    19381 agctgctcag gagccgttgt accaacagcc gcaacccgtt gaacagcagc ctgttgtgga
    19441 gcctgaaccc gttgtagaag agacaaaacc cacgcgtccg ccgctttact attttgaaga
    19501 agtggaagag aagcgagccc gtgaacgtga acaacttgcg gcctggtatc aaccgattcc
    19561 agaaccggtt aaagaaccgg aaccgatcaa atcttcgctg aaagcacctt ctgttgcagc
    19621 agtacctcca gtagaagccg ctgccgctgt ttctccgctg gcatctggtg tgaaaaaagc
    19681 gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag
    19741 cggtggaccg cgtccgcagg tcaaagaggg gattggcccg cagttgccac gaccgaaacg
    19801 catccgcgtg ccaactcgtc gtgaactggc gtcttacggt attaagctgc cctcacagcg
    19861 tgcggcagaa gaaaaagccc gcgaagccca gcgtaatcag tacgattctg gcgatcagta
    19921 caacgatgat gaaatcgatg cgatgcagca ggatgaactg gcacgtcagt tcgcccagac
    19981 gcagcagcaa cgctatggcg aacagtatca acatgatgtg cccgtgaaca cagaagatgc
    20041 agatgctgcg gcagaggcag aactggctcg tcagtttgct caaactcaac aacaacgtta
    20101 ttccggcgaa caaccggctg gggcgaatcc gttctcgctg gatgattttg aattttcgcc
    20161 aatgaaagcg ttgctggatg atggtccaca cgaaccgctg tttacgccaa ttgttgaacc
    20221 tgtacagcag ccgcaacaac cggtagcacc gcagcagcaa tatcagcagc cgcaacaacc
    20281 ggtagcaccg cagccgcagt atcagcagcc gcaacaaccg gttgcaccgc agccgcagta
    20341 tcagcagccg caacaaccag ttgcgccaca gccgcaatat cagcagccgc aacaaccggt
    20401 tgcaccgcag cagcaatatc agcagccgca acagccggtt gcacagcagc cgcaatatca
    20461 gcagccgcaa cagccagttg cgccgcagcc gcatgatacc ctgcttcatc cgctgttgat
    20521 gcgcaatggt gacagccgtc cgttgcataa accgacgacg ccgctgcctt ctctggattt
    20581 gctgacacct ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg aacaaatggc
    20641 gcgcctggta gaagcgcgtc tggctgattt ccgtattaaa gccgatgttg tcaattactc
    20701 tccggggcca gttatcactc gctttgaatt gaacctggca ccgggcgtaa aagcagcgcg
    20761 catttctaac ttgtcacgtg accttgcccg ttcactttcg acggtggcgg tgcgtgtcgt
    20821 tgaagttatt cctggcaaac cctatgtagg tctggagtta ccgaataaaa aacgacaaac
    20881 cgtttatctg cgcgaagttt tggataacgc caaattccgc gataatccgt cgccattaac
    20941 cgtggtgctg ggtaaagata tcgccggtga gccggtggtt gccgatctgg cgaaaatgcc
    21001 gcacttgttg gttgcgggga ctaccggttc cggtaagtct gtcggggtga acgcgatgat
    21061 cctgagcatg ctttataaag cacagccaga agatgtgcgt ttcatcatga tcgacccgaa
    21121 aatgctggag ctttcggttt atgaaggcat tccgcatctg ttaacggaag tcgttaccga
    21181 tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac gagatggagc gtcgttataa
    21241 actgatgtct gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa ttgctgaagc
    21301 cgataggatg atgcgtccga ttccagaccc gtactggaag ccgggtgaca gtatggatgc
    21361 ccagcatccg gtgctgaaaa aagaaccgta cattgtggtg ttggtggacg aatttgccga
    21421 cctgatgatg acggtaggta aaaaagtgga agagctgata gcacgtctgg cgcaaaaagc
    21481 ccgtgccgcg ggtatccacc tcgtactggc aacgcagcgt ccatcggttg atgttattac
    21541 tggtctgatt aaagcgaata ttccgacccg tatcgccttt accgtatcca gtaagattga
    21601 ctcacgtacc attcttgatc aggctggtgc ggaatcactg ctgggtatgg gggatatgct
    21661 ctactctggg ccgaactcca cgttgccggt acgtgtccat ggtgcttttg ttcgcgatca
    21721 ggaagttcat gccgtggtgc aggactggaa agcgcgtggt cgcccacagt atgttgatgg
    21781 catcacctcc gacagtgaaa gcgaaggtgg cgtgggcggt ttcgatggcg ctgaagaact
    21841 ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag cgtcaatttc
    21901 tggcgtacag cgtcagttcc gcattggtta taaccgtgca gcgcgtatta tcgaacagat
    21961 ggaagcgcag gggattgtca gcgaacaggg gcacaacggt aatcgtgaag tgctggcccc
    22021 accgccattt gactaattaa tgcaacgtag accggataag acgcgatagc gtcgcatccg
    22081 gtattctatc aactgaaaat tcagtatttt cttctttcct caagctgatt attagcctgg
    22141 aatagagagt agagggaact cccgattggg agtgacgtaa tttgaggaat aatgatgaaa
    22201 aaaattgcca tcacctgtgc attactctca agcttagtag caagcagcgt ttgggctgat
    22261 gccgcaagcg atcttaaaag ccgcctggat aaagtcagca gcttccacgc cagcttcaca
    22321 cagaaagtga ctgacggtag cggcgcggcg gtgcaggaag gtcagggcga tctgtgggtg
    22381 aaacgtccaa acttattcaa ctggcatatg actcaacctg atgaaagcat tctggtttct
    22441 gacggtaaaa cactgtggtt ctataacccg ttcgttgagc aagctacggc aacctggctg
    22501 aaagatgcca ccggtaatac gccgtttatg ctgattgccc gcaaccagtc cagcgactgg
    22561 cagcagtaca atatcaaaca gaatggcgat gactttgttc tgacgccgaa agccagcaat
    22621 ggcaatctga agcagttcac cattaacgtg ggacgtgatg gcacaatcca tcagtttagc
    22681 gcggtggagc aggacgatca gcgtagcagt tatcaactga aatcccagca aaatggggct
    22741 gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg tcacggtaga tgatcaacgt
    22801 aagtagaggc acctgagtga gcaatctgtc gctcgatttt tcggataata cttttcaacc
    22861 tctggctgcg cgtatgcggc cagaaaattt agcacagtat atcggccagc aacatttgct
    22921 ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg catctgcatt ctatgatcct
    22981 ctgggggccg ccgggtaccg gcaaaacaac cctcgctgaa gtgattgccc gctatgcgaa
    23041 cgctgatgtg gaacgtattt ctgccgtcac ctctggtgtg aaagagattc gcgaggcgat
    23101 cgagcgcgct cggcaaaacc gcaatgcagg tcgccgcact attctttttg ttgacgaagt
    23161 tcaccgtttc aacaaaagcc agcaggatgc atttctgcca catattgaag acggcaccat
    23221 cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgag cttaattcgg cactgctttc
    23281 ccgtgcccgt gtctatctgt tgaaatccct gagtacagag gatattgagc aagtactaac
    23341 tcaggcgatg gaagacaaaa cccgtggcta tggtggtcag gatattgttc tgccagatga
    23401 aacacgacgc gccattgctg aactggtgaa tggcgacgcg cgccgggcgt taaatacgct
    23461 ggaaatgatg gcggatatgg ccgaagtcga tgatagcggt aagcgggtcc tgaagcctga
    23521 attactgacc gaaatcgccg gtgaacgtag cgcccgcttt gataacaaag gcgatcgctt
    23581 ttacgatctg atttccgcac tgcataagtc ggtacgtggt agcgcacccg atgcggcgct
    23641 ctactggtat gcgcgaatta ttaccgccgg tggcgatccg ttatatgtcg cgcgtcgttg
    23701 cctggcaatt gcttccgaag atgtcggcaa tgccgaccca cgggcgatgc aggtggcaat
    23761 tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa ggtgaacgcg ccattgctca
    23821 ggcgattgtt tacctggcct gtgcgccaaa aagcaacgct gtctacactg cgtttaaagc
    23881 cgcgctggcc gatgctcgcg aacgtccgga ttatgacgtg ccggttcatt tgcgtaatgc
    23941 gccgacgaaa ttaatgaagg aaatgggcta cgggcaggaa tatcgttacg ctcatgatga
    24001 agcaaacgct tatgctgccg gtgaggttta cttcccgccg gaaatagcac aaacacgcta
    24061 ttatttcccg acaaacaggg gccttgaagg caagattggc gaaaagctcg cctggctggc
    24121 tgaacaggat caaaatagcc ccataaaacg ctaccgttaa tgttatcgtt gcggtaatgt
    24181 tgttaatgta tccctgtggt cgcaggctgt ggccacatct cccatttaat tcgataagca
    24241 caggataagc atgctcgatc ccaatctgct gcgtaatgag ccagacgcag tcgctgaaaa
    24301 actggcacgc cggggcttta agctggatgt agataagctg ggcgctcttg aggagcgtcg
    24361 taaagtattg caggtcaaaa cggaaaacct gcaagcggag cgtaactccc gatcgaaatc
    24421 cattggccag gcgaaagcgc gcggggaaga tatcgagcct ttacgtctgg aagtgaacaa
    24481 actgggcgaa gagctggatg cagcaaaagc cgagctggat gctttacagg ctgaaattcg
    24541 cgatatcgcg ctaaccatcc ctaacctgcc tgcagatgaa gtgccggtag gtaaagacga
    24601 aaatgacaac gttgaagtca gtcgctgggg caccccgcgt gagtttgact ttgaagttcg
    24661 tgaccatgtg acgctgggtg aaatgcactc tggcctcgac tttgcagccg cagttaagtt
    24721 gactggttcc cgctttgtgg taatgaaagg gcagattgct cgcatgcacc gcgcactgtc
    24781 gcagtttatg ctggatctgc ataccgaaca gcatggctac agtgagaact atgttccgta
    24841 cctggttaac caggacacgc tgtacggtac gggtcagctg ccgaaatttg ctggcgatct
    24901 gttccatact cgtccgctgg aagaagaagc agacaccagt aactatgcgc tgatcccaac
    24961 ggcagaagtt ccgctgacca acctggtacg cggtgaaatc atcgatgaag atgatctgcc
    25021 aattaagatg accgcccaca ccccatgttt ccgttctgaa gctggttcat atggtcgtga
    25081 cactcgtggt ctgatccgta tgcaccagtt cgacaaagtt gaaatggtgc agatcgtgcg
    25141 cccggaagac tcaatggcgg cgctggaaga gatgaccggt catgcggaaa aagtcctgca
    25201 gctgctgggc ctgccgtacc gtaaaatcat cctttgtacc ggcgacatgg gctttggcgc
    25261 ttgcaaaact tacgacctgg aagtatggat cccggcacag aacacctacc gcgagatctc
    25321 ttcctgctcc aacgtttggg atttccaggc acgtcgtatg caggcacgtt gccgtagcaa
    25381 gtcggacaag aaaacccgtc tggttcatac cctgaacggt tctggtcttg ctgttggtcg
    25441 tacgctggtt gcagtaatgg aaaactatca gcaggctgat ggtcgtattg aagtaccaga
    25501 agttctgcgt ccgtatatga acggactgga atatattggc taatacccaa tttttctgaa
    25561 tctaaaaagc gcctgcgggc gctttttttg tctccctttg ataccgaaca ataattactc
    25621 ctcacttaca agtaatacta ctttcgagtg aaaatctacc tatctctttg attttcaaat
    25681 tattcgatgt atacaagcct atatagcgat ctgctataga aataattaca caatacggtt
    25741 tgttactgga atcaatcgtg agcaagcttg agtgagccat tatgaaaacg aaaatccctg
    25801 atgcggtatt ggctgctgag gtgagtcgcc gtggtttggt aaaaacgaca gcgatcggcg
    25861 gcctggcaat ggccagcagc gcattaacat taccttttag tcggattgcg cacgctgtcg
    25921 atagcgctat tccaacaaaa tcagacgaaa aggttatctg gagcgcctgt acagttaact
    25981 gtggtagtcg ctgcccgcta cgtatgcacg tagtggacgg tgaaatcaaa tatgtcgaaa
    26041 cggacaatac cggcgatgac aattacgacg gcctgcacca ggttcgcgcc tgtctgcgtg
    26101 ggcgttccat gcgtcgccgt gtctacaatc cggaccgcct gaaatatccg atgaaacgag
    26161 tcggggcgcg cggtgaaggc aaattcgagc gcattagctg ggaagaagcc tacgacatca
    26221 tcgcgaccaa aatgcagcgc ctgatcaaag agtacggcaa cgagtctatc tatctgaact
    26281 atggcaccgg tacgctgggc ggcaccatga cccgctcgtg gccgccggga aataccctgg
    26341 tcgcgcggct aatgaactgc tgcggcggtt atctgaacca ttatggcgac tactcctccg
    26401 cgcaaattgc ggaaggtctg aactatacct acggcggctg ggcagatggc aacagcccgt
    26461 cggatatcga aaacagtaaa ctggtagtgc tgtttggtaa taaccctggc gaaacgcgaa
    26521 tgagtggcgg tggggtgact tactatcttg aacaggcgcg ccagaaatct aatgctcgca
    26581 tgatcatcat cgatccgcgc tataccgaca ccggtgccgg gcgtgaagat gagtggatcc
    26641 ctattcgtcc gggaacagat gccgcactgg ttaacggtct ggcgtatgtc atgatcaccg
    26701 aaaacctggt agatcaggaa ttcctcgata aatattgcgt tggctacgac gagaaaaccc
    26761 tgccagccag tgcgccgaaa aatggtcact ataaagctta tattctgggt gaaggacccg
    26821 atggcgtggc taaaacaccg gaatgggcct cgcaaatcac cggtgttccg gcagacaaaa
    26881 tcatcaaact ggctcgtgaa atcggcagta ccaaaccggc gtttatcagc cagggatggg
    26941 gcccgcagcg tcatgctaac ggtgaaatcg caacccgtgc tatctcgatg ctggcgattc
    27001 tgaccggtaa cgttggtatt aacgggggga acagcggcgc gcgcgaaggt tcatacagct
    27061 taccgtttgt ccgtatgccg actctcgaaa acccgatcca gaccagcatt tctatgttta
    27121 tgtggaccga tgccattgaa cgtggcccgg aaatgacggc gctgcgtgat ggtgtgcgcg
    27181 ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt aactgcctga
    27241 ttaatcagca ttctgaaatc aaccgtaccc atgaaatcct tcaggatgat aagaagtgcg
    27301 agctgattgt ggttatcgac tgccacatga cctcatcggc gaaatatgct gacatcctgc
    27361 tgcctgactg caccgcttcc gaacagatgg actttgcact ggatgcatcc tgcgggaata
    27421 tgtcttacgt tattttcaat gatcaggtca ttaaaccgcg ctttgaatgt aagactatct
    27481 atgagatgac cagcgaactg gcaaaacgtc ttggcgtcga acaacagttt actgaaggtc
    27541 gtactcagga agagtggatg cggcatctgt atgcccagtc gcgggaagcg attcctgaac
    27601 tgccaacgtt tgaagagttc cgtaagcagg ggatctttaa aaagcgcgat ccgcaagggc
    27661 atcacgttgc ttacaaagcc ttccgtgaag atccgcaggc taatccattg accacgccat
    27721 cgggcaaaat tgagatctat tcacaggcgc tggctgacat tgccgctacc tgggaattgc
    27781 cagaaggtga tgtgatcgat ccactgccga tctatacgcc gggctttgaa agttatcagg
    27841 atccgctgaa caaacagtat ccgctgcagc ttacgggttt ccactataaa tctcgcgttc
    27901 actcaactta cggcaacgtt gatgtgctga aagcagcttg ccgtcaggaa atgtggatca
    27961 acccgcttga tgcgcaaaaa cgcggtatca gcaacggcga taaagtcagg atctttaatg
    28021 atcgtggtga ggttcatatt gaggcgaaag tgacgccacg aatgatgccg ggtgtggtcg
    28081 cactgggtga aggtgcctgg tatgacccgg atacaaaacg tgtcgataag ggcggttgta
    28141 ttaacgtact gaccactcaa cgtccgtctc ctcttgccaa ggggaatcca tcacatacga
    28201 accttgttca ggttgaaaag gtgtaaggag taaccgatga caacccagta tggatttttt
    28261 attgattcca gccgttgcac cggttgcaaa acctgcgagc tggcttgtaa agactacaaa
    28321 gatttgacgc cagaagtcag cttccgccgc atttatgaat atgctggtgg cgactggcag
    28381 gaagataacg gtgtctggca ccagaacgtg tttgcctact atctgtcgat ttcatgtaac
    28441 cactgcgaag atccggcttg tactaaagtc tgcccgagcg gtgcgatgca taaacgtgaa
    28501 gatggttttg ttgtggtcga tgaagatgta tgcattggct gccgttactg tcatatggct
    28561 tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc atatgaccaa atgcgatggt
    28621 tgttatgacc gtgttgcgga gggaaaaaaa ccgatctgcg tagagtcctg cccgctgcgg
    28681 gcgctggatt tcggccctat cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt
    28741 gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc caatgccaac
    28801 agccgcccaa ccggggatac cactggctat ctggcaaacc cgaaggaggt gtgagatggg
    28861 aagtggatgg catgaatggc cgctgatgat cttcacggtc ttcgggcaat gtgtagcggg
    28921 tggttttatc gttctggctt tggcgctgct caaaggcgac ctgcgagcag aagcccagca
    28981 gcgtgttatc gcctgcatgt tgggtttatg ggggctgatg ggcattggct ttatcgcctc
    29041 tatgcttcat cttggttcac cgatgcgcgc ttttaactcg ctcaatcggg taggggcttc
    29101 agcactcagt aacgaaattg ccagcggttc gatctttttt gctgtaggcg gcatcggctg
    29161 gttgctggca atgctgaaaa agctttcacc ggcattgcgt acgctttggc tggtggtgac
    29221 gatggttctt ggcgtagtct ttgtctggat gatggtgcgt gtgtataaca gcatcgatac
    29281 agtacccacc tggtacagca tctggacacc gatgggcttc ttcctgacga tgtttatggg
    29341 cggcccgtta ctgggttacc tgttgctgag tctggctggt gtcgatggct gggcgatgcg
    29401 tctgctgcca gccatttctg ttctggcact ggtagtgagt ggcgtgatgt cagtgatgca
    29461 gggcgcagag ctggcgaata ttcatagctc tgtgcagcag gccgcagcgc tggtgccgga
    29521 ctatggtgcg ctgatgtcct ggcggatcgt gcttttggcc gttgccctgt gcttgtggat
    29581 tgcaccacag ctaaaaggtt atcagcctgc ggttccgcta ctttctgtat cattcattct
    29641 gcttctggca ggggaattaa tcggtcgtgg cgtattctat ggtttgcata tgaccgtggg
    29701 gatggctgtc gcaagttaat cagtaacaac cggggtttcg gccccggttc gcttttattt
    29761 ctgcttcgtt aacgtgtcat aactggtcat caagttacga taatcaggaa tgtggttaga
    29821 gaacaacgtc gccagtcctt caatatcatt acgccagtcg cgatgcagct cgcacgccac
    29881 gccgaaccat gtcatcaatt gcgcaccagc ttgcgacatg cgatcccatg ccgaatgccg
    29941 ggtaatttca ttaaaggtcc ctgacgcgtc agtcacgaca aagacgtcaa acccttcttc
    30001 aatggctgaa agcgccggga atgccacgca aacttcggtt accacaccgg caataattaa
    30061 ctgtttttta cctgtcgctt tgacagcttt tacaaaatct tcgttatccc aggcgttaat
    30121 atttcccggg cgagcaatgt aaggtgcatc ggggaattgt gctttcagtt ctggaactaa
    30181 tgggccgtta ggaccggttt caaaactggt ggtaagaata gttggtaagt tgaaatactt
    30241 agccaggtca cccagcgcca gcacgttatt tttaaactta tcgggttcga tatctcgtac
    30301 aagggaaagt aaaccagcct ggtgatcaac aagcaaaacg gcagcatcat ttttatcaag
    30361 acgaacatac ggtttggtca tcctcttctc ctttcgagag tcagtggtac gtctaaatcg
    30421 gtcagacgtg atgcattgct ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat
    30481 gaaatttttc gactgaacga agcgtatgaa atttgtgtta gatcaataaa aacaatcaga
    30541 tgagattaat cgtctcttca gcattagcgt tatgaatgag taatataaat cgataaaacc
    30601 gccgctgcga tatgcgcgta ttttcaccca ttgacaatgt ttttggcggt ggcatgatgc
    30661 gcatgaaatt tgaacttcct cacggtttta attcatgtcc acgtataccc ggcctgtcat
    30721 gcttttgctg tctggcctgc ttttgttgac tctggcgatt gcggtgttaa atacactcgt
    30781 gccgctttgg ctcgcccagg aacacatgtc tacatggcag gtaggcgttg tcagctcatc
    30841 ctattttacc ggcaaccttg tcggtacatt gctgacaggg tatgtcatta aacgcattgg
    30901 ctttaaccgt agctattatc tggcctcctt catttttgcc gctggctgtg ccggccttgg
    30961 cctgatgatt ggtttctgga gttggttggc gtggcgtttt gtcgcgggcg tcggctgtgc
    31021 catgatttgg gtagtcgttg agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg
    31081 gcgtttgctt gctgcataca tgatggttta ttacgtggga acgtttttag gccagttact
    31141 ggtcagcaaa gtctcaaccg agttgatgag tgtattgccg tgggttacag ctttgacgtt
    31201 ggcaggaatc ttaccgctgt tgtttacgcg tgtcctgaat cagcaggctg aaaaccatga
    31261 ttcgacgtca attacgtcaa tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg
    31321 cattatctca ggaatcgtgc tgggatctct atatggcctg atgccgctgt acctcaatca
    31381 caaaggggtg agcaatgcca gcattggttt ctggatggcg gtactggtca gtgcgggtat
    31441 cctcgggcaa tggccgattg gacgtctggc ggataagttt ggtcgactgc tggtgttgcg
    31501 tgttcaggtc tttgtcgtca ttctcggcag tattgcgatg cttagccagg cggcgatggc
    31561 cccagcgtta ttcatcctcg gtgccgctgg ctttacgcta tatccggtgg cgatggcctg
    31621 ggcttgtgag aaagttgaac atcatcaact ggtagcgatg aaccaggcct tactgttgag
    31681 ctataccgtg ggaagcctgc ttggcccgtc atttaccgct atgctaatgc agaatttctc
    31741 cgataattta ttatttatca tgatcgccag cgtatcgttt atctatttgc tgatgctgct
    31801 tcgtaacgcc ggtcatacgc cgaaacccgt ggctcacgtg taaatgaatt caagcagagt
    31861 gtgaacttac tgtttcacac tctgcttttc tgtgcacaaa aaagcggcaa cttaataaag
    31921 ttgccgcttt acggggaaat tagaacatga ccttatgacc gtactgctca agaatgcctt
    31981 tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg tattcttcac
    32041 ccattgccac ccatttgtgt ttgcccagtt cgtggtaggg gaggagctcg attttctcaa
    32101 cgttgcccat atcacgggta aattcaccaa ggcgatgcgc tgaatcgtca tcgtcagacc
    32161 agcctgggac aacaacatag cggatccaca ccttcacatt tttgttcgcc agatatttag
    32221 cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc tcgtcgttca
    32281 tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca atcaccggat
    32341 cgtaacgacg aacaaaaccg ttggtgtcca gacaggtatg aatgccttct tttttgcagg
    32401 cgcggaacca gtcacgaaca aactcagctt gtaggattgc ctcaccgccg gatgcggtaa
    32461 cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc atcaaatctt
    32521 caacggtaac ttctttaccg ccatgcgtat cccaggtgtc gcggttatga caatacaggc
    32581 agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acccgggccg tctacggttc
    32641 cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt ctccagatgt
    32701 ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct gttttggcag
    32761 tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag gccccacttt
    32821 cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta attacataga
    32881 ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg agttgaaacg
    32941 tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt tttccatcgc
    33001 gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac cttcgatgga
    33061 tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac gaacttcgtc
    33121 gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat ctttagcgta
    33181 agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct ggtcacgacc
    33241 gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt ctggggtgtt
    33301 accagttttc ttaccataca caacgttaga agtgatggtc agaacagact gagtcgggat
    33361 agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta ccaggtcaac
    33421 agccaggtca tctacacgcg gatcgttgtt accaaactgc gggtattcgc cttcgatttc
    33481 gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat atttgattgc
    33541 agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg tgcggataac
    33601 gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt gcatgtagtg
    33661 gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt gatccatgcg
    33721 ctccatcact tcatcatagt tcaggacatc gcctttgatc ggttcagact tcggaccaac
    33781 ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg ttttcgccag
    33841 gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc ttacgcagca
    33901 agcaatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt tctcatactg
    33961 cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca gcggcagttt
    34021 ttcagaccac agaatggtca tgttcggttc cggagacgga cccatggtgt acagggtgtt
    34081 caggaaacgg aagctgtttt tggtaaccag ggtacgaccg tcgaggccca taccaccgat
    34141 agattcggtt gcccagatcg ggtcgccaga gaacagttca tcgtattccg gagtacgcag
    34201 gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg cttcttgttc
    34261 ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg tggaggtacg
    34321 accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc cgaagtaagt
    34381 ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc cgtatttcgc
    34441 agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt cgcgcagacg
    34501 gatagtctgt tccaggttta cgccgttttc cagatcagcc tgcagagagg tgaactgtgc
    34561 aagtttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt agtcaccgat
    34621 gatacgacca cggccataag catctggcag accggtcaga acaccggatt tacggcaacg
    34681 caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac ggtattcagt
    34741 gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg aaccttcgat
    34801 cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag tctgcagacc
    34861 aacgattttc tcaagctgct tgttgatgta accagcgtcg tgagaggtga tggtggaagc
    34921 aacagcggtg tcaaagtcaa ctggcgcgtg agtgcggttt tccagtttaa cgccttccat
    34981 tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg actcgtcacc
    35041 ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat tctgccagtc
    35101 acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg acatgtaaca
    35161 cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt aatggtgatc
    35221 gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac cgccgataat
    35281 attaccgatc gtaaccggaa tcaggttatc agtgatgaag ttcatcacgg tcaggtgaga
    35341 aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga agtcgcggat
    35401 tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac cgctggcaac
    35461 aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc ggccagaata
    35521 actcatccat actgccagac ataccatcag gtttgccagg ataccaagac agacggcctc
    35581 aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc cccattgacc
    35641 atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca gtgcgccgac
    35701 caggttgcca aaataaacat ttagccagtt tttcgccaac tgaccccagg tgatgcgccc
    35761 actcgccttg gcaacaacaa tcaacactgt ggaagtaaag agatcggctc cgcagacaac
    35821 acaaagaatc aaccccagag agaagcaaat gccgccaacc agttttgcca tgccgaaggg
    35881 cattgtacct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg agatgaaaac
    35941 accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt tatagacacc
    36001 tgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag ggttgtcagc
    36061 tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag cattatagat
    36121 gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg cat
//
LOCUS       DAZKHK010000041        35404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKHK010000041 DAZKHK010000000
VERSION     DAZKHK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.041"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            258..848
                     /locus_tag="ACHOS5_002013"
     CDS             258..848
                     /locus_tag="ACHOS5_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9776830.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            957..1697
                     /locus_tag="ACHOS5_002014"
     CDS             957..1697
                     /locus_tag="ACHOS5_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9776831.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1732..4320
                     /gene="htrE"
                     /locus_tag="ACHOS5_002015"
     CDS             1732..4320
                     /gene="htrE"
                     /locus_tag="ACHOS5_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFP9776832.1"
                     /translation="MTIKSTNHLTHIATFCALLYSNSALCAELVEYDHTFLMGKDASN
                     IDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFAVIEGKKNAQACITQKNLLQFHI
                     SSPDKNSEKAILLKRDDDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMKNYQN
                     YVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYAAFNGGINLGAWRLRASGNYNW
                     MTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRMLPPV
                     LASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVTIEEA
                     DGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNNYLTG
                     YTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWNKLFE
                     NTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVTVSIN
                     QLLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLNEDGQ
                     TDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTNRISY
                     SVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFVLHSG
                     GLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRIALDI
                     NDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADIYDEQ
                     NNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSIILNG
                     LRCQIQ"
     gene            4337..4903
                     /locus_tag="ACHOS5_002016"
     CDS             4337..4903
                     /locus_tag="ACHOS5_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9776833.1"
                     /translation="MIKITPHKITILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     GTNVTDNGKDKYTLVIPSMGMDKIANKTAQSEANFKLVANGCSSGISWIDTTLTGNQS
                     GSSPALIIPLASDTTSTTSYIGMGFKRKATSGDTFLKPNSAEYIRWSASEISTDGLEM
                     TVALRETSVGKGVPGKFRALATFNFSYQ"
     gene            4915..5523
                     /gene="yadL"
                     /locus_tag="ACHOS5_002017"
     CDS             4915..5523
                     /gene="yadL"
                     /locus_tag="ACHOS5_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP9776834.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGTTKISTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTTR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTLGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTNVTAGDFTAPATFTVTYN"
     gene            5550..6146
                     /locus_tag="ACHOS5_002018"
     CDS             5550..6146
                     /locus_tag="ACHOS5_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9776835.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNVSAENYHPGGKEFTITVQDCTLQGTGNVLNQLHINF
                     RALSGVMATGSSQIFANEDNAGAKNVGVVIFSIQDPANTFNVLSATGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGPLISHVLVDIYYE"
     gene            6172..7464
                     /locus_tag="ACHOS5_002019"
     CDS             6172..7464
                     /locus_tag="ACHOS5_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFP9776836.1"
                     /translation="MQHDNGVKMKTFFRYFLFLALCSCCYTASAGTDDNVGYIVGNNY
                     GVGPGDQKWRETGPNGDVTVKFRYGSGTNNLVFYKPTQLGPTGVSLKWAQLDSASGGG
                     FLYCNRSGSTSGGPMDIENKMVDSGKSYGGHKLFKTSVPGLYYTLAISNIWSTLTSTD
                     INPSGMYIGDSTSQSFNWRGESEQTLYWSCNNANSSNKYWAVGGVMQTLTIEFYTDTN
                     FNPTTNQRVTLSRTDNYLYSFKAYGAGSGINSHSYFLKIDFDLTDIILTNPTCFTAAL
                     SGPSVSGSTVKMGDYSPAQIKNGATAVPFDITLQNCIRVRNIETKLKSNKVGSVSKEL
                     LANTLTGNDAAKGVGVLIEGLKNTKSAQMVLKPNDATSIYKDYETENDTTGGIFPDNG
                     NAGTSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7576..8370
                     /gene="panB"
                     /locus_tag="ACHOS5_002020"
     CDS             7576..8370
                     /gene="panB"
                     /locus_tag="ACHOS5_002020"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9776837.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8382..9233
                     /gene="panC"
                     /locus_tag="ACHOS5_002021"
     CDS             8382..9233
                     /gene="panC"
                     /locus_tag="ACHOS5_002021"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9776838.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRSDDIQIRDADTLLEISENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(9315..9518)
                     /locus_tag="ACHOS5_002022"
     CDS             complement(9315..9518)
                     /locus_tag="ACHOS5_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9776839.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYKEGLQKVRLAQRLLSKGM
                     SREDVAEVTALSLAEIDKLINSN"
     gene            complement(9587..10519)
                     /locus_tag="ACHOS5_002023"
     CDS             complement(9587..10519)
                     /locus_tag="ACHOS5_002023"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9776840.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVVRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVRQEVIQEVRQEV
                     RQEYALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            10793..11173
                     /gene="panD"
                     /locus_tag="ACHOS5_002024"
     CDS             10793..11173
                     /gene="panD"
                     /locus_tag="ACHOS5_002024"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9776841.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(11177..12406)
                     /gene="yadE"
                     /locus_tag="ACHOS5_002025"
     CDS             complement(11177..12406)
                     /gene="yadE"
                     /locus_tag="ACHOS5_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9776842.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGVIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPMLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12470..12910)
                     /gene="yadI"
                     /locus_tag="ACHOS5_002026"
     CDS             complement(12470..12910)
                     /gene="yadI"
                     /locus_tag="ACHOS5_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9776843.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLTSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(13015..13785)
                     /gene="yadH"
                     /locus_tag="ACHOS5_002027"
     CDS             complement(13015..13785)
                     /gene="yadH"
                     /locus_tag="ACHOS5_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9776844.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13782..14708)
                     /gene="yadG"
                     /locus_tag="ACHOS5_002028"
     CDS             complement(13782..14708)
                     /gene="yadG"
                     /locus_tag="ACHOS5_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9776845.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            14817..15479
                     /gene="can"
                     /locus_tag="ACHOS5_002029"
     CDS             14817..15479
                     /gene="can"
                     /locus_tag="ACHOS5_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP9776846.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHIN
                     HK"
     gene            complement(15520..16056)
                     /gene="hpt"
                     /locus_tag="ACHOS5_002030"
     CDS             complement(15520..16056)
                     /gene="hpt"
                     /locus_tag="ACHOS5_002030"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9776847.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            16262..18652
                     /gene="gcd"
                     /locus_tag="ACHOS5_002031"
     CDS             16262..18652
                     /gene="gcd"
                     /locus_tag="ACHOS5_002031"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP9776848.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18699..20249)
                     /gene="cueO"
                     /locus_tag="ACHOS5_002032"
     CDS             complement(18699..20249)
                     /gene="cueO"
                     /locus_tag="ACHOS5_002032"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP9776849.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPSGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAITPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20415..20762
                     /gene="yacC"
                     /locus_tag="ACHOS5_002033"
     CDS             20415..20762
                     /gene="yacC"
                     /locus_tag="ACHOS5_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP9776850.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20868..21734
                     /gene="speE"
                     /locus_tag="ACHOS5_002034"
     CDS             20868..21734
                     /gene="speE"
                     /locus_tag="ACHOS5_002034"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP9776851.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21750..22544
                     /gene="speD"
                     /locus_tag="ACHOS5_002035"
     CDS             21750..22544
                     /gene="speD"
                     /locus_tag="ACHOS5_002035"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP9776852.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22582..22944)
                     /gene="yacL"
                     /locus_tag="ACHOS5_002036"
     CDS             complement(22582..22944)
                     /gene="yacL"
                     /locus_tag="ACHOS5_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP9776853.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVED
                     AARTLKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(23119..25716)
                     /gene="acnB"
                     /locus_tag="ACHOS5_002037"
     CDS             complement(23119..25716)
                     /gene="acnB"
                     /locus_tag="ACHOS5_002037"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP9776854.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            26071..27828
                     /gene="yacH"
                     /locus_tag="ACHOS5_002038"
     CDS             26071..27828
                     /gene="yacH"
                     /locus_tag="ACHOS5_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP9776855.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVIAIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRDGMPYHDQDMAKRFHQTDVKGGMSA
                     TPLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHNGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAAREKMQTNPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(28070..29494)
                     /gene="lpdA"
                     /locus_tag="ACHOS5_002039"
     CDS             complement(28070..29494)
                     /gene="lpdA"
                     /locus_tag="ACHOS5_002039"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9776856.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29702..31594)
                     /gene="aceF"
                     /locus_tag="ACHOS5_002040"
     CDS             complement(29702..31594)
                     /gene="aceF"
                     /locus_tag="ACHOS5_002040"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9776857.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMIFEVAGEAGAAAPAAKQEAAPAAAPAPATGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31609..34272)
                     /gene="aceE"
                     /locus_tag="ACHOS5_002041"
     CDS             complement(31609..34272)
                     /gene="aceE"
                     /locus_tag="ACHOS5_002041"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP9776858.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34433..35197)
                     /gene="pdhR"
                     /locus_tag="ACHOS5_002042"
     CDS             complement(34433..35197)
                     /gene="pdhR"
                     /locus_tag="ACHOS5_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP9776859.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8971 a         9199 c         8447 g         8787 t
ORIGIN      
        1 ccgaaattct aattccatac accactccct taatttttta aagttaattt tactaatttt
       61 catatttagt aaaattaaat tgcatatgtc atggcattat gcaaaatagc tgtcccaatg
      121 atgaatgaat gtaatactta gtaaaaaaca aacaaacatc attaacaaag taacgttcag
      181 ttacatggca ttgcatcaaa atctcatcat catgcaatgc tgttatctgc tttatggaat
      241 attactcaag gatttttatg tctaaaaaat taggttttgc gctgtccggt attatgttgg
      301 caatggctgc tggtactgca tttgctgcta acgatatgga cggcggtcaa ttaaatatta
      361 gcggtctggt cgttgataac acttgtgaag ctcgtgttga tggcggtaac aaagacggtc
      421 tgatcctgct gcaaaccgct actgttgctg aaattactga tggtgtactg gataccactg
      481 taggcgctaa agctaaacca ttcagcatta ctattgactg cagcaaagct aacccggctc
      541 cgggtactac tgcaaaaatg accttcggtt ctgttttctt tggtaacagc aaaggaacac
      601 tgaacaacga catgtctatt aacaccccgt ctgacggcgt aaatatcgct ctgcacaata
      661 ttgaaggttc tactattaaa caggttcagg tcaataatcc gggtgatgtt tacagcaaaa
      721 ctctggattc aacgtctaaa tctgcaacct acgattttaa agcatcctat gttcgtgcgg
      781 atgcaagcaa agcagctact gccggttatg taaaaaccaa ctcagcatat accattactt
      841 accagtaaat atcaaccgct taaattgatc gggtgaatat atacagggaa taacatcaaa
      901 tatttttact tttgataatt attccctgtt ttaattaatt ctgtaaggaa tggtttatgt
      961 tttttaatac taaacatacc gcagcattgt gcttagcgag ctgtatggct tttagctcat
     1021 cagctattgc agacatcgta atttcaggta cacgcgtagt ttataaaagc gaacaaaaaa
     1081 gcgtcaatgt acgtctggaa aataaaggca ataacccatt gctggttcag agttggttag
     1141 atactggaga tgataacgct gaaccaggca gtattactgt cccctttacc gctacgccgc
     1201 cagtatcacg tattgatgct aaacgtggcc aaacaatcaa actgatgtac accgccagca
     1261 gtgctctgcc aaaagacaga gaaagtgtgt tctggtttaa cgtactggaa gtacctccaa
     1321 aaccagatgc agcaaaagca gcaaaccaaa gcctgctaca actggcattt cgcacacgta
     1381 taaaactgtt ctatcgcccg gaaggattga gtggaatttc atctgatgca ccattggctc
     1441 tgaagtggtc ctgggcaaca tctgaaggca aagcagcatt acgcgttgat aaccctacgc
     1501 cctactatgt ctcttttagc agtggtgatt tagaagccaa cggcaaacgc tatcccctgg
     1561 atatgacaat gattgcgccg ttcagtaacg acgtcatcaa ggtaaatggg atgagtggca
     1621 gaacaagctc tgcaaaagtg cattttttcg ccattaatga ttttggtgga tctattgaag
     1681 gcaacgccaa cctttaatct ggcaaggata taacacagca ggaagcagaa cgtgactatt
     1741 aaatctacta atcaccttac ccacatcgcc acgttttgcg ctctgctgta tagcaatagt
     1801 gctttatgcg ctgaactcgt cgaatatgac catacatttc tgatgggaaa ggatgcttca
     1861 aatatcgacc ttagtcggta tacagaaggc aatcccacac tacctggcat ttacgatgtc
     1921 agtgtgtatg ttaacgatca acccatcatg agccaaagca ttgcttttgc tgttattgaa
     1981 ggtaaaaaga acgctcaggc ctgcattacg cagaaaaatt tattgcaatt tcatatcagt
     2041 tcaccagaca agaatagcga aaaagccatt ttgcttaagc gagacgacga tcttggtgat
     2101 tgcctcaatc tggctgaaat gatccctcaa tcatccattc gttacgatgt taacgatcaa
     2161 cgcctggata tcgacgtccc ccaggcatgg attatgaaga attatcaaaa ctatgttgat
     2221 ccctctttgt gggaaaatgg cattaatgca gccatgcttt cctacaacct caacggatat
     2281 cacagtgaaa gtccaggcag aacaaatgac agtatctacg cggcattcaa cggtgggata
     2341 aacttaggtg cctggcggtt acgtgcctcg ggtaactaca actggatgac caatgtccat
     2401 agcgattacg attttcagaa tcgctattta caacgcgatc ttgcgtcact acgttcacaa
     2461 ctggttattg gtgaatctta cacgactggc gagaccttcg attcagtcag cattcgcggt
     2521 attcgcttat acagtgacag tcgaatgctg cctccggtat tagccagttt tgcgccaatc
     2581 attcatggcg tagcgaatac caatgccaaa gtaaccgtta tgcaaaatgg ttataagatc
     2641 tatgaaacga cggtgccgcc aggagctttt gccattgacg atcttagccc gtcaggttat
     2701 ggcagcgatc tcattgtgac tatcgaagaa gctgatggca caaaacggac gttctcccag
     2761 ccattttcat ctgttgtgca aatgctgcgc cctggcgttg gtcgctggga tattagcgcc
     2821 ggtcaggttt taaaagacag tatccaggat gagcctaatt tatttcaagc cagctactac
     2881 tatggtttga ataactacct gacaggatat accggtattc agctaactga taataactac
     2941 accgccggat tgctggggct tggtatgaat acccccgtgg gggctttttc tgtcgatgtt
     3001 actcactcaa atgtcagcat tccggacgat aaaacctatc aaggacaaag ctaccgtatt
     3061 tcctggaaca agttatttga aaataccagt acatcattaa atatcgcggc ctatcgctac
     3121 tccacccagc attatctggg cctcaatgat gcactgacgc tgattgatga agtagaacat
     3181 cctgagcaag atcttgagcc taaatccatg cgcaattact cgcgcatgaa aaatcaggtc
     3241 acggtcagta tcaaccaact gctgaaattt gagaaaaagg attatggttc attttatctc
     3301 tcgggaagct ggtcagatta ctgggcctcc gggcaaaaca gtactaatta ctctattggt
     3361 tatagcaata gtgcgtcctg gggcagttac agtatcagtg ctcaacgttc gttgaatgaa
     3421 gatggtcaaa ccgacgatag catttatctc agcttcacaa ttccaataga aaatttactg
     3481 ggtacggaac accgcagctc cggcttccag agtattgata cacaattaaa cagcgacttt
     3541 aagggtaata accaactcaa tatcagcagt agtggttaca gcgataccaa tcgcatcagc
     3601 tacagcgtaa acacgggtta tatgatgaat aaatccagtg atgatttgag ttatatcggc
     3661 ggttatgcca gttatgaatc gccgtgggga acgctgtcag gttcagcctc agcaagcagt
     3721 gataatagtc gccaattctc actcaatact gatggtggat ttgtgttaca tagcggcggg
     3781 ctgacgttca gtaatgacag ttttagtgat tccgacactc tggctgtcat tcaggcgcca
     3841 ggagcaaaag gcgctcgtat taactacgga aacagcaccg tagaccgctg gggttatggt
     3901 gtgaccagcg ccctttcgcc ataccatgaa aatcgtatcg cactggacat taacgatctg
     3961 gaaaacgatg ttgaattgaa aagcaccagt actgtcgccg tcccgcgcca gggagccgtc
     4021 gtctttgctg attttgaaac agttcaggga caatcggcaa ttatgaacat cgtgcgaagc
     4081 gatggtaaaa atattccatt tgcagcggat atttatgatg aacaaaacaa tatcatcggc
     4141 aacgtaggtc agggtggtca ggcctttgtt cgcggtatcg ggcaggaagg aaatatacga
     4201 attacctgga ttgaagaggg caaaccggtc agttgttttg ctcattatca acaaaacacg
     4261 acatcagaaa aaatagcaca atctattatt ctgaacggac ttcgttgtca gatacagtaa
     4321 ccacaaggga cttccaatga taaaaatcac accacataaa ataacaatac tcatgggact
     4381 attgttatcg ccttcggtat ttgcaacgga tgttaatgtc gattttaccg ctaccgtgaa
     4441 agcaacaacc tgtaatatta cacttaccgg aactaatgtc actgataatg gaaaagataa
     4501 gtacacctta gttattccct cgatgggaat ggataaaata gccaataaaa cagcacaatc
     4561 tgaagccaac tttaaattgg tagccaatgg atgcagcagt ggaattagct ggattgatac
     4621 aaccctgaca ggaaatcaat caggaagttc acccgcgcta attatccccc tggccagtga
     4681 tacaacctcc accacaagct atatcggaat gggatttaaa aggaaggcga cttcaggcga
     4741 tacatttctg aaacctaata gcgcagaata tatacgttgg agtgcatcag aaattagcac
     4801 cgatggactt gagatgactg tcgcactgcg tgaaacaagt gtagggaaag gtgttccagg
     4861 caagtttcgc gcactggcaa cttttaactt cagctatcaa taacaaggat gacaatgatg
     4921 atattaaaaa aattatgtta tggattatcg ggtagtctat ttctggcttc atcactgtta
     4981 accatttcct cacatgcggc cactgacagt attggcctga ctgttactac tgacgtagaa
     5041 atgggtacct gtacatcaac gttgactgat gacggtacaa ctaaaatttc caccatcaat
     5101 ttcggtgatg tatatatttc tgaaattaat gccgaaacta aaattaaaac cttcaaatta
     5161 cagttcaaag actgtgcggg gattcccggg aaaaaggcaa aaataaaatt gactacacga
     5221 gcattatgtg aaggtaattc taataatggt cctggatttg ctaacgcatc aacggcaaca
     5281 gcaaaagcag cggcggtcgc cgttgaggtc tggagcacat ctacgctagg taagaacggc
     5341 gctaaacaat tcagttgtgt cacccccgca acagaagaag tcagtattgc taatgcaacg
     5401 ggcagcaatg ttgtcgatta cccaatgagt gcggtgttgg tggtcgcaaa agataaaact
     5461 gtcacaaatg tcactgcggg tgatttcact gccccggcaa cattcaccgt gacttataac
     5521 taacaggcag actgattcag gaactaaata tgcgtcccac tcaacgtaat ctgatgaaaa
     5581 aatcaattct gattctgtcg ttgctattca gcgccacttc ccctgtggtt gcaggacagg
     5641 atgttgacat tgttgcaaat ataaaaaata acacctgcca aagtggaatc agcaacaacg
     5701 gaaatattga tttaggtgtc gttggtgtgg gcgattttac cggaaacgtt tcagccgaaa
     5761 attatcaccc cggtggaaaa gagttcacta ttacagtaca ggactgcaca cttcagggca
     5821 cagggaatgt gttaaatcag ttacatatta attttcgcgc actcagtggc gtaatggcga
     5881 caggttccag tcaaatattc gctaatgaag acaacgccgg ggctaaaaat gttggggtag
     5941 ttatattttc tattcaggac ccggcaaata ccttcaacgt tcttagtgcc actggtagct
     6001 cacgttccgt ttatccggtc atgtcatcag cactaaataa ttcatcctgg aaatttagta
     6061 cccgaatgca aaaaatcgat ccagcactga atgtcacctc tggcccactg ataagccatg
     6121 tgttagtgga tatctactac gaataacaga caaagtgcta caagtattat cttgcagcat
     6181 gacaatggag taaaaatgaa gacattcttc agatactttc tttttttagc actatgctct
     6241 tgttgttata cagcaagcgc cggcaccgat gataacgtcg gctatattgt tgggaataat
     6301 tacggtgttg ggcctggaga ccaaaaatgg cgcgaaacag gccccaatgg agatgttacc
     6361 gtcaaattcc ggtatggttc aggcaccaat aatttagttt tttataaacc aacccaactt
     6421 ggcccaaccg gggtgtcatt aaaatgggcg cagttagatt ccgcttcagg tggaggtttc
     6481 ctttactgta acaggagtgg tagcacaagc ggcgggccaa tggatattga aaataaaatg
     6541 gttgattccg gcaaaagtta tggcggacat aaactcttta agacatctgt tccaggcctg
     6601 tattacacgt tagcaatatc aaacatttgg tcaacactta catccactga tatcaaccca
     6661 tcaggcatgt atattggcga ttctacgagc caatctttta actggcgtgg cgaaagcgag
     6721 caaacactat attggtcttg caacaacgcg aatagcagca acaaatactg ggccgttggt
     6781 ggcgttatgc aaacgctcac gattgaattt tataccgata ccaactttaa cccgacaacg
     6841 aatcaacggg taacattatc cagaacagat aattatctct actcattcaa agcatatggt
     6901 gctggcagtg gtatcaatag ccacagctat tttcttaaaa tagactttga cctgacagat
     6961 atcatattaa caaaccctac ctgttttact gcggcgctta gtggcccatc ggtaagtggt
     7021 tcaacggtta aaatgggtga ttatagccct gcacaaataa aaaatggcgc aacagcggtt
     7081 ccttttgata tcacgctaca gaactgtatt cgtgttcgca atatagaaac aaaactcaag
     7141 tcaaacaagg tgggtagtgt aagcaaagaa cttctggcta acacactcac aggaaatgac
     7201 gcagcaaaag gcgttggtgt actgattgaa ggtttaaaaa acactaaaag tgcacaaatg
     7261 gtactgaaac caaatgatgc aacgtctatt tataaagatt atgaaacaga aaatgacact
     7321 actgggggga ttttcccgga taatggcaac gctgggacct ctcaacctct tcatttccag
     7381 gctacattaa aacaagacgg taatattgct atagaaccgg gggattttaa agccaccagc
     7441 acttttcagg ttacctaccc ctgataacac tattgcaccg ccagacaacg gcggtgcgat
     7501 acggataaat cattgccccc accaatgtga ctgataaaat gcgagacagc acaacatcat
     7561 ttatcaggat acgctatgaa accaaccacg attgcttcat tgcagaaatg caagcaggac
     7621 aaaaaacgtt tcgcaaccat caccgcttac gactatagct tcgcaaaact gtttgccgaa
     7681 gaaggactta acgtcatgct ggtcggcgac tcactgggca tgacggttca ggggcatgac
     7741 tccacgctac cggttaccgt tgccgatatc gcctaccaca ctgctgccgt acgtcgcggt
     7801 gcgccaaact gcctgctgct ggctgatctg ccgtttatgg cgtatgccac accagaacaa
     7861 gcctttgaaa acgccgcgac tgtcatgcgt gctggggcca acatggtcaa aattgaaggc
     7921 ggtgagtggc tggtggaaac cgtacaaatg ctgaccgaac gtgccgttcc tgtttgtggt
     7981 cacttaggtt taaccccaca gtcagtgaat attttcggtg gctacaaagt ccaggggcgc
     8041 ggcgatgaag cgggcgatcg actgctcagc gatgcattag ccttagaagc tgctggggca
     8101 caactgctgg tactggagtg cgtgccggtt gaactggcaa aacgcattac cgaagcactg
     8161 gcgatcccgg ttatcggtat tggcgcaggc aacgtcactg acgggcaaat cctcgtgatg
     8221 cacgacgcgt tcggcattac cggcggtcat attcctaaat tcgccaaaaa tttcctcgcc
     8281 gaaacgggcg acatccgcgc ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt
     8341 tacccgggcg aagaacacag tttccattaa ggagtgacgt tgtgttaatt atcgaaaccc
     8401 tgccgctgct gcgtcagcaa atccgccgcc tgcgtatgga aggtaagcgc gtggcgctgg
     8461 tgcccaccat gggtaacctg cacgacggcc atatgaagct ggtcgacgaa gccaaagccc
     8521 gcgccgatgt ggtcgtcgtc agtattttcg ttaacccaat gcagtttgac cgcccggaag
     8581 atctggcacg ttatccacgg accttgcaag aggactgcga aaagctgaac aaacgtaaag
     8641 tggatttagt tttcgcgcct tcggtaaaag agatctaccc gaacggtact gaaacccaca
     8701 cctatgttga tgttcccggt ctttccacca tgctggaagg tgccagccgt ccgggacatt
     8761 ttcgcggcgt ttcgactatt gtcagcaagc tgttcaacct ggtccagcca gatatcgcct
     8821 gcttcggtga aaaagatttt cagcaactgg cgctgatccg caaaatggtt gctgatatgg
     8881 gctttgatat tgagattgtc ggtgtgccaa ttatgcgcgc caaagacggt ctggcgctga
     8941 gttcccgtaa cggctatctg acggcggaac aacgcaaaat tgcgcccggt ctgtacaagg
     9001 ttttaagttc gattgccgat aaattacagg ctggcgaacg ggatctcgat gaaattattg
     9061 ctattgccgg gcaagaactg aatgaaaaag gtttccgttc cgatgatatt cagattcgcg
     9121 atgccgacac cttgctggaa atctctgaaa acagcaaacg agcggtgatt ctggtagccg
     9181 cctggcttgg cgatgctcgc ctgatcgaca acaaactagt cgaactggcg taatgcttaa
     9241 ctggcgctac ggctgatggc gccagttatt cattgccacg ttatctgtag gcaattattc
     9301 atatcgtaga caacttagtt agagttaatt agcttatcaa tctctgcaag agataaagct
     9361 gtcacctctg caacgtcttc ccgagacatt cctttactca gaagacgctg ggccagtctt
     9421 accttctgga gtccctcttt atatcccttc tgaaatccct tctcttcaaa ccactgcgcc
     9481 aacgtctgcc tggtttctct ccgtgtcttt atttccatat gttcccttaa ttatctggtc
     9541 agttaacaat aacaattatc tggctataac tgataatgca gataacttag ctaatcagct
     9601 tatcaacctc agcaagaggt aaatttgcca tctctgcaac gtcttcccga gacatccctt
     9661 tactcagaaa acgcagggcg tattcctgtc ttacttcctg tctaacctct tgtattacct
     9721 cctgtcttac ctcctgtctt acctcctgcc tttcctcctg tcttcccttc tcttcaaacc
     9781 actgcgccag cgtcatcata gactcccctc ccgtttccct gtctctcaca acaccataaa
     9841 acaaatccgc ttgttcagta tgaccgcgtt gcagcatata gttttgcatg gcaactaact
     9901 gacttccgct agtgtaccct tcgtcaatca gcgtgaccag ttgctccagc aataacatta
     9961 agtcgcgctg gcgaatatgt ttttgcagca gttcgagaat cgcaatccgc cgatgttgca
    10021 tgatttcgtc atccggcgta atggtgatgt ccaccagcgg gaaaggactg ttatagacgc
    10081 gtcgcgccag ttccggtgag taaaacatat caaaccagca cattgatagc ggataaggtg
    10141 tggcctcgcc ctgataaaac agaattggca ccaccagcgg cagcttatcg tggtcagcct
    10201 ccagatgccg gtgcatggca gcaatagaat aacgcatcat gcgaaaggcc attttcttat
    10261 ccggcttgct ttgatgttca atcacaacat gcaaataacc ggggttgccc tgcatttgca
    10321 cggaataaag cacgtcagtg ctgtgccctt tcaggctttc ttcaataaaa ctccccgact
    10381 caagatgcag cgtgtcgagg tcacacagtt cgcgtagctc cgctggcaaa tggatatcca
    10441 gaaagtcgcg agccgtttcc gcatgcatta aaaattgttt aaacaccgca tcatgcggtg
    10501 tggtactcgg tgcatccatc ttccctgcct tccgtgtggt cttgcgtgaa ggcagtatcc
    10561 agtcatggtg tgccgtcgtc agccgcctct ttagtgttta ccgagcagcg ttcagataaa
    10621 tttcaattgg ttacaggcgt tacatggcac gcttcggcac ggtacgcaaa ctgctgctgc
    10681 aaatgccgtc agatgagtag acactaaaca aaaatcgggc aatactgcgt gagaatttcc
    10741 tcaaagcagg catcgcctgc ttcgttaacg acagggtaga aaggtagaag ttatgattcg
    10801 cacgatgctg cagggcaaac tccaccgcgt gaaagtgact catgcggacc tgcactatga
    10861 aggttcttgc gccattgatc aggattttct tgacgcagcc ggtattctcg aaaacgaagc
    10921 cattgatatc tggaatgtca ccaacggcaa gcgtttctcc acctatgcca tcgcggcaga
    10981 acgcggttcg agaattattt ctgttaacgg tgcggcggcc cactgcgcca gcgtcggcga
    11041 tattgtcatc atcgccagct tcgttaccat gccagatgaa gaagctcgca cctggcgacc
    11101 caacgtcgcc tattttgaag gcgacaacga aatgaaacgt accgcgaaag cgattccggt
    11161 tcaggttgcc tgattgttat ccctgcggct ggttactcac cagccgcgac atcgtctcca
    11221 gcgaatccgt tcttaagata taaagtcgtt ttagtaacaa cggattatcc cccggtttta
    11281 ctttgccttt catggtcgtc accgcgaggt gaaatccggc ttcttttgct gccttcacgg
    11341 ctttgtcatt aaatccgcca aatggataag aaagatacaa aacatgtgga ttaaattgcg
    11401 ccaaagcgcg gcgtgaacgt gcaaaatcaa acagaatatt gtgctcacta cggctcagca
    11461 atataggccg ccgataacca tctatccgat gcaaaaaatg agtatgtgac tggaaatcaa
    11521 atacatcgcg aatttcgtta agttcagaaa cgctcataaa ttgcagcgat tttgggttcc
    11581 atttctgcgg gtgacgtttg atgcgcgagg taacaataaa cgccgtcgcc ttcataccat
    11641 attgtttcaa cacaggatac gcatagcggc tcaccgactt gaggccatca tcaaaggtaa
    11701 ttaccaccgc tcgcgcaggg agattgatct tattcttcac gtaaccttcc agctgcgcca
    11761 tgctcagtgt cgcgtatccc ctgtcgcgca gccaggccat ctggttattg aaagcgcgta
    11821 ccgaggtggt cgtcgaagta tggcgaaaac gggtgttttc ttcgtcgcgc aaaatatggt
    11881 gataggttag catcggcagg ccattatcgg gttgggcatc cagcgcgctg atataggcca
    11941 atcgatcgcc aatacggatc tgataccagg tttgatttaa cctgtctttc agtttatgca
    12001 aaatcgggta gcgcaaattg tccgccagta ctccaaatgg cgcactgccc acactcggcg
    12061 cgttataaac cggcgtatct ttccaggtta ttaagttctg attactcagc ggcttgttga
    12121 ggtcacccaa accatcttca actttttgtc gcccctgaac cggctcgaga tggcctttat
    12181 cgataacccc tttaccaaag ccaaaattaa atacgtaata acttgcggca gtgggctcca
    12241 cggcaatgat ttgcccggcc cgaatattcc ctaccgtcac cattttgtcg ccaatttgcg
    12301 cccagaccgc ggcattttcg atggtttgca tataacgggc aggtaacgcg gcactgacac
    12361 ttgcggtaaa cagcatcagc aggagaataa cagcttgttt gtacatgaaa aaataaccga
    12421 gggtgaaaca acgcttttat tgtagcaaaa gcgtcatcag aatcgcgggt taatactcat
    12481 acaaattatg tttgaggacg aaaggcggat ttttttgttg ttggtgccaa agactactca
    12541 cctccggcgc acccagttcg acaatatgct cgcgaaacgc tgaactggtc atggtttcac
    12601 ggcaagccat catctgttca attaacggta acgtgacacc agaaatcact tcgcaacggg
    12661 agtgtttgtg gcttaataat gaagccacgc ggtacggtgg cgctccggct atatcggtta
    12721 aaaagataac gccctcacca gagtccgttt catgcaaggc atcgcacatc atgcggctga
    12781 gcatattaga ggttaatccg cgccagaaat tcacggcccg gcactgaaga agtgccccat
    12841 gttttttctc cagcgcatcc agtatctctt gcgcccggtc atcgtgacag gtaattaccc
    12901 aacctaacat cgccttcctc cttagccgaa aatcagtcta ccctgaccat tgttgataaa
    12961 gggtgatagc cgtcaaaact cctctccccc aaatccagag gagaggaaat agccttagct
    13021 acgcaaacca cgtccacgtt ggatcagcga ccaacagatc aaataaaacg ccacaataaa
    13081 gaccaccagt acgccaaagg tagtgaccag cggaacatca ttgataccga ggaagccgta
    13141 gcggaaacca ctgatcatat aaacgattgg gttcaggtgc gacagccctt gccagaacgg
    13201 cggcaacaaa gtcagggagt aaaacacccc gcccaaatac gtgagtggcg ttaacacaaa
    13261 ggttggcacc aggctgatgt catcgaacgt tttggcaaac acgccgttca gcaaacccgc
    13321 aagggagaat agcaccgcag tgagcaccag ggttaaggca acgaataccc acgaatgcac
    13381 ctgaaacggc acaaaaaaca gtgaaattgc cgtcaccaaa ataccaacaa acagaccacg
    13441 cgccacgcca ccaccgacat atccggcaat aatgacatga gtcggaaccg gcgctaccag
    13501 cagctcttca atattacgct ggaacttggc accaaaaaat gatgacgcga cgttagcgta
    13561 ggcgttggtg atcaccgaca tcatgatcag ccccggtacg atgaactgca tgtagctgaa
    13621 gccatgcata tcgccaatac gcgaaccaat caggttaccg aagataataa agtaaagggt
    13681 catggtgatg actggcggca ccagcgtctg cacccagata cgcataaagc gatggatctc
    13741 tttcgcccag atgcttttta gcgccaccca gtaaagatgc atcatgtgcg atctccttgt
    13801 ttttcattaa ccagtgaaac aaacagctct tccagacggt tagctttgtt acgcatactt
    13861 aatacctgaa tgccctgctc acttaactgc gtaaatacgc tgttgatgcc ctgctcacgc
    13921 agcacttcaa cttccagcgt ggcggtatct accagtcgat actgatagcc atcgagcttc
    13981 ggtaacggac ttttcggtgc gagatcgaga ataaaggttt ctgatttcag cttcgccagc
    14041 agcgccttca tcgaagtatt ttccaccaac tcaccgtgtt gaataatgcc gatattgcgg
    14101 cacagcattt ctgcttcttc caggtagtgt gtggtgagaa tgatggtggt gcctttgtcg
    14161 tttaaatcct tcaaaaagcc ccacattgag cggcgaagtt caatatccac gcctgcggtc
    14221 ggttcgtcga gaatcagtag tttaggttca tgcattaacg cacgggcaat cattaaacgg
    14281 cgcttcatcc cgccagataa catacgcgca cgttcgttgc gttttcccca tagatcgagt
    14341 tgtttaagat acttttcgct gcgaatgtac gcttctttgc gctccacacc gtagtaccct
    14401 gcctgattca ccacaatttg ctgcacggtt tcaaacgggt tgaagttaaa ttcctgtggc
    14461 accagaccca actgacgttt agcgtttacg acatctttct cgagatcgta accaaatacg
    14521 ctgacccgcc cggaggtttt atttaccaga gagctgataa taccgatagt ggtcgatttt
    14581 ccggccccgt tcggcccgag aagcgcataa aaatcacccg cttcgacctg caaatcgatt
    14641 ccacgaagcg cctgaacgcc gcctggatag gtttttttaa gctgttgaag ttccagtgca
    14701 atggtcataa atttttactt accttacgtt cttacacttt atctgtggtt taaatcgtcc
    14761 cggagttgcc ctatattagc caaacgtaat tatttggtta caggtcgtta acctccatga
    14821 aagacataga tacactcatc agcaacaatg cactatggtc aaaaatgctg gtggaagagg
    14881 atcccgggtt ttttgagaaa ctggcacaag cgcaaaaacc gcgctttcta tggattggat
    14941 gttccgacag tcgcgttcct gcagaacgtt taaccggtct tgagccgggc gaactctttg
    15001 ttcaccgtaa tgttgctaac ctggtcattc acaccgacct gaactgcctt tccgtggttc
    15061 agtatgcagt ggatgtactc gaagttgaac acattattat ctgtggccac tacggttgcg
    15121 gcggcgtaca agccgcagtt gaaaacccgg aactggggct tatcaacaac tggctgctgc
    15181 atatccgcga tatctggttc aaacatagct cattgctcgg cgaaatgccg caagagcgcc
    15241 gtctggatac cttgtgtgaa ctgaacgtca tggaacaggt gtataacctg ggccactcca
    15301 ccattatgca atcagcgtgg aaacgcgggc agaaagttac cattcacggc tgggcctacg
    15361 gcattcacga cggcttgctg cgtgatctgg atgtcacagc gactaaccgc gaaacccttg
    15421 agcaacgtta ccgtcacggg atctccaacc tcaagctgaa gcacatcaat cacaaataaa
    15481 ttcgccatgc cggatgcaac acatccggca acttcacact tactcgtcca gcagaatcac
    15541 tttgccgata tacggcagat gacggtaacg ctgtgcgtaa tcaatgccgt aacccaccac
    15601 aaactcatcc gggatcgaga aaccgataaa ttctaccggg acgttcactt cacgacggga
    15661 cggtttatcc agcagcgtac aaatcgccaa cgacttcggt tcgcgcaggc ttaagatctc
    15721 acgcactttc gacagtgtat tccccgagtc gatgatatct tcaacaatca gcacgtcctt
    15781 gccacggata tcttcatcca gatctttgag gattttcaca tcacgggtgg tggacatgcc
    15841 gctaccgtag ctggaggcgg tcataaagtc gacttcatga gatacctgaa cttcacggca
    15901 caggtccgcc ataaacataa atgagccacg cagcagaccc accagcacca tatcgctgcc
    15961 gctgtctttg taacgctcag taatctgacg acccagttcg gcgatacgcg ctttaatctc
    16021 cgcttcgggg atcattactt ctacagtatg tttcatatct ataaccatat gatttaaaaa
    16081 caaatcactt cgcacgggca ggtttcagca ccgcaacaaa gcgcaagaca cgcagtatac
    16141 cagtaaaact attcattcgc ggaatctgtt aataaagctc gaattgtgat gacgatcaca
    16201 catgttaaaa cctataatta attgctacta aaatattaat gaattgaaat ggtgtctctt
    16261 tatggcaatt aacaataccg gctcgcggcg attactcgtc acgctaacag ccctttttgc
    16321 agcgctttgc gggctgtatc tactcattgg cggaggctgg ctggtcgcga ttggcggctc
    16381 ctggtactac cctatcgctg gccttgtgat gctcggcgtc gcctggatgc tgtggcgcag
    16441 taaacgcgcc gcgctttggc tgtacgcagc cctgctgctc ggcactatga tttggggcgt
    16501 ctgggaagtt ggtttcgact tctgggcgct gactccgcgc agcgacattc tggtcttctt
    16561 cggcatctgg ctgatcctgc cgtttgtctg gcgtcgcctg gtcattcctg ccagcggcgc
    16621 agttgccgca ctggtggtcg cactgctgat tagcggtggt atcctgacct gggccggatt
    16681 taacgatccg caggaaatca gcggcacctt aagcgccgat accacgccag cagaagccat
    16741 ctcccccgtt gccgatcagg actggcctgc ctatggtcgc aatcaggaag gtcaacgctt
    16801 ttcaccactg aagcaaatta acgctgataa cgtccacaag ctgaaagaag cctgggtgtt
    16861 ccgtactggc gatgtgaagc agccgaacga tccgggcgaa atcaccaatg aagtgacgcc
    16921 aattaaagtg ggcgacaccc tttacctgtg taccgcgcat caacgtctgt ttgcgctgga
    16981 tgccgccagc ggtaaagaga aatggcatta cgatcctgag ctgaaaacca acgagtcctt
    17041 ccagcatgta acctgccgtg gcgtctctta tcatgaagcc aaagcagaaa ctgcttcgcc
    17101 ggaagtgatg gcggattgcc cgcgtcgtat cattcttccg gtcaatgatg gtcgtctgat
    17161 tgcaattaac gctgaaaacg gcaagctgtg cgaaaccttc gccaataaag gcgtgctcaa
    17221 tctgcaaagc aatatgccgg acaccaaacc gggtctgtat gaaccgactt cgccgccgat
    17281 tatcaccgat aaaaccatcg tgatggccgg ttcagtcacc gacaacttct caacccgcga
    17341 aacgtctggc gtgatccgtg gttttgatgt caacaccggg gagctgctgt gggcttttga
    17401 tccgggcgcg aaagatccga atgcgatccc gtctgacgaa cacaccttta cctttaactc
    17461 accaaactcg tgggcaccag cggcctatga cgcgaagctg gatctggtct atctgccgat
    17521 gggcgtcacc acgccagata tctggggtgg taaccgcaca ccggaacagg aacgttatgc
    17581 cagctcgatt ctggcgctga atgccactac cgggaaactg gcgtggagct atcagaccgt
    17641 tcaccacgac ctgtgggata tggatcttcc ggcacagccg acgctggcgg acatcaccgt
    17701 taatggtcag aaagtgccgg ttatttacgc tccggcgaaa accggcaaca tttttgtgct
    17761 cgatcgtcgt aatggtgaac tggtggttcc tgccccggaa aaaccggttc cccaaggtgc
    17821 tgctaaaggc gattacgtga cccctactca acccttctcg gaactgagct tccgtccgac
    17881 gaaagatttg agcggtgcgg atatgtgggg agccaccatg tttgaccagt tggtgtgccg
    17941 cgtgatgttc caccagatgc gctatgaagg cattttcacg ccgccatctg aacagggtac
    18001 gctggtcttc ccgggtaacc tggggatgtt cgaatggggc gggatttccg ttgatccgaa
    18061 tcgtgaagtg gcgattgcca acccaatggc actgccgttt gtttcgaaac tgatcccacg
    18121 cggtccaggc aacccgatgg aacagccgaa agatgccaaa ggcacgggta cggaatccgg
    18181 catccagccg cagtacggtg taccgtatgg tgtcacgctc aacccgttcc tctcaccgtt
    18241 tggtctgcca tgtaaacaac cagcatgggg ctatatctca gcattggatc tgaaaaccaa
    18301 tgaagtggtg tggaagaaac gtattggtac gccacaggac agtatgccat tcccgatgcc
    18361 tgtaccggtg ccgttcaata tgggtatgcc gatgcttggc gggccaatct ccacggcggg
    18421 taacgtgctg tttatcgccg ctacggcaga taactacctg cgcgcttaca acatgagcaa
    18481 cggtgaaaaa ctgtggcagg gtcgtttacc agcgggtggt caggctacgc caatgaccta
    18541 tgaagtgaat ggcaagcagt atgtggtgat ctccgcaggc ggtcacggtt catttggtac
    18601 gaagatgggc gactatattg tggcttatgc gttgccggat gatgtgaagt aaccataatg
    18661 cccggagaga tccgggcata tttccgaata cggtcttttt ataccgtaaa ccctaacatc
    18721 atccccgtat cttcatgctc cagcagatgg cagtgcgcca tataagcatg ttctttcggt
    18781 gcatcgtgat taaacttcac cagcacttcg ctgacattac cttctacctt aacggtatct
    18841 ttccagcctg cgcgatgcgt cgctggcggt ttgccatttt ctgacaagat acggaactgc
    18901 gtaccgtgga tatggaacgg atgcagcatc atgtcgccca cgccagagat aacccaacgt
    18961 tcgtactgcc ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc ctgaccgttg
    19021 attttgttgg catggtggaa atcaaacttc ccgctgtggt tcatatgatt catattgccg
    19081 tgccccatat ggcccatcat ctggctgtga tccatcccgg ccatcgcctg atcgccatat
    19141 ttctccatca gcatctgcat ccccatcata tcgagcattg ggtccataga aagttgcagc
    19201 ttgcgtaccg tcagcccttc cagcgaaggt aacgcaggca ggctacttaa tgtgtctggc
    19261 aaagcaccag aggcactaat ggcaatcggc tgaatccgca ttaccggatg cggcttgtca
    19321 aacggcgtaa tcgccatccc catctggctg accggcagcg tcaccaggtc aaagggtttg
    19381 ttgtcgttaa cctccaccag cacttcaaaa cgctcgccca tcagcactgg cagctcgttc
    19441 accttcaccg gttcaggtag cagaccgccg tcgctggcaa tcacataaag cgggcgattg
    19501 tcgctggtgg cgaaattgag cgagcgggca ttacagccat tgagcaaacg caggcgcagc
    19561 cagccacgcg gggcagcgtg ttgcgggtag attgcaccgt tggtcagcaa cgtatcgcca
    19621 aaccaaccca cagcggcggt catcacatcc agttgataat caatctgccc gtcagcgtta
    19681 aattttttat cctgaacaat caccgggaca tcatcaatac cccactgttt tggcagcatt
    19741 aatttcagga tctcgtcatc ttcaatcaca accaaaccag ccagccccat cgccacctgt
    19801 cgcccggtct tgccatgttg atgtggatgg aaccagcagg tagcggcagg ttgatcaacg
    19861 ttcaacgtca ccgagcgctt gccacctgac ggaataattc cctgcgggcc gccgtccact
    19921 tcacccggta cttccagccc gtgccagtgc aacgtcgtct cttccgtcag ttggttgtag
    19981 atatcaaccg ttaccgcttt gccacgctgt aatttcaccg ccggccccag cagattgccg
    20041 ttatagcccc aggtagttgc ggttttcccg ccaaaggtgg actgacctgc gccaatagtt
    20101 aactgaatgc gattacgggc atcggtcgtg agcaaatcag ggatcggtaa cgttgggcgt
    20161 tccgccgcaa atactgcgcg gctccacagc ggcaaggctg aagccacacc cagcgcgacg
    20221 gaatatttca agaaatcacg acgttgcata gttatttcct tattcttaag caggcgaaac
    20281 aaaatcaaac gttgagcata gtccttcccc ttacgggaag gtcaagccgc agacacaatt
    20341 taatcgacaa taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc ttctttaagc
    20401 ccctggtaga agcaatgaag acgtttttca gaacagtgtt attcggcagc ctgatggccg
    20461 tttgcgcaaa cagttacgcg ctcagcgagt ctgaagccga agatatggcc gatttaacgg
    20521 cagtttttgt ttttctgaag aacgattgtg gttaccagaa cttacctaac gggcaaattc
    20581 gtcgcgcgct ggtctttttc gcccagcaaa accagtggga tctcagtaat tacgacacct
    20641 tcgacatgaa agccctcggt gaagacagct accgcgatct cagcggcatt ggcattcccg
    20701 tcgctaaaaa gtgcaaagcc ctggcccgcg attccttaag cctgcttgcc tacgtcaaat
    20761 aatccctgat acctttttgt agaaatattg accgtgcatc cgcggtcaat gttagctatt
    20821 atgttgcgcc atttttttac gggtgttaac aaaggaggta tcaacccatg gccgaaaaaa
    20881 aacagtggca tgaaacgcta cacgaccagt ttgggcagta ctttgcggta gataacgttc
    20941 tgtatcatga aaagaccgat caccaggatc tgatcatttt tgagaacgcc gcatttggtc
    21001 gcgtaatggc gctggatggt gtagtacaaa ccaccgagcg tgacgagttt atctatcatg
    21061 agatgatgac ccatgttccg ctactggccc acggtcacgc gaaacatgtg ctgattatcg
    21121 gcggcggcga cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt gagtcaatca
    21181 cgatggtgga aatcgatgcg ggtgtcgtgt cgttctgccg tcagtatctt cccaaccata
    21241 acgccggtag ttacgacgat ccgcgcttta aactggtgat cgacgatggt gtcaatttcg
    21301 ttaatcaaac cagccagacc tttgatgtca ttatctccga ctgcaccgat cctatcggtc
    21361 ccggcgaaag tctgtttact tctgcatttt atgaaggctg caaacgttgc ctgaatcctg
    21421 gcggtatctt cgtcgcacaa aacggcgtct gctttttaca gcaggaagag gccatcgaca
    21481 gccatcgcaa actcagccat tacttcagcg atgttggctt ttatcaggcg gcgatcccga
    21541 cctattacgg cggcatcatg acctttgcat gggcgacaga taacgacgcc ttacgccatc
    21601 tctcaaccga aattattcag gcgcgttttc tcgcttctgg cctgaaatgc cgttattaca
    21661 atccggcagt ccatacggca gcttttgcct taccccagta tctgcaagat gcactggctt
    21721 cacagccgtc ctaaggagaa gataagaaat tgaaaaaact gaaactgcat ggctttaata
    21781 atctgaccaa aagtctgagt ttttgtattt acgatatctg ctacgccaaa actaccgaag
    21841 agcgcgacgg ttatattgct tatatcgatg aactctataa tgccaaccgt ctgaccgaaa
    21901 tcctgtcaga aacctgttcc attatcgggg ccaatattct taacatcgcc cgccaggatt
    21961 acgagccaca aggtgccagc gtcactattc tggtgagtga agaaccggtc gacccgaaac
    22021 ttatcgacaa aacagaacac cccggcccac tgccagaaac ggtcgttgcc catctcgata
    22081 aaagtcatat ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt ttatgtacct
    22141 tccgcgccga tattgaagtc tctacctgcg gcgtgatttc tccgctgaag gcgctgaatt
    22201 acctgatcca ccagcttgag tccgatattg tgactattga ttatcgcgtg cgcggtttta
    22261 cccgcgacat taacggtatg aagcacttta tcgaccatga gattaattcg attcagaact
    22321 ttatgtctga cgatatgaag gcgctgtatg acatggtgga tgtaaacgtc tatcaggaaa
    22381 atatcttcca taccaagatg ttgcttaaag agttcgacct taagcactac atgttccaca
    22441 ccaaaccgga agatttaacc gacagcgagc gccaggaaat taccgctgcg ctgtggaaag
    22501 aaatgcgcga gatttattac gggcgcaata tgcccgctgt ttaacgtctc aggcggagca
    22561 cggaggctcc gcctgattta cttacttctg ctgcacgaaa ttacggtaag ccgcaaccac
    22621 ctgaagaaaa tcctcaacgc cgcacagcga caagctctct tcatcgtagt agttcatccc
    22681 ctcttccatt tcatcgccag cgaattccag ttgattggcg cgaaccatca cctcttcacc
    22741 gtccatccac agagtgtatt catgccctgc ccgctgccag gatcgttcgc tgcctttgag
    22801 cgtgcgcgct gcgtcttcca cttcatcaag caaggccagg ttttctttca cctcttcatt
    22861 aaaccagtgc ccgaccactt catgccccat ggacatgcgc acctttacca ctccggtaat
    22921 atcgcgcaga aattcgtaat ccatagtgtg ttcctctgct cccggcaatg cgccgaaatc
    22981 gcattgcgcc attgatgtaa ttatcgcagc cttaccggag aaaaacagcg gaacggcgat
    23041 ggtttagaaa taaaaaatgc ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc
    23101 agcgcgtgcg cttacttatt aaaccgcagt ctggaaaatc accccgtccg ctttttcggt
    23161 gtactgagaa agctggtcga agttcagata acggtaagta tcaacggctg ttttatctac
    23221 ctgcgccacg taggtctggt actcttccgg cgtcggcagt ttgccaatca gcgccgcaac
    23281 agcagccagt tccgcagaag ccaggaagac attcgcgcca gtacccaaac ggttcgggaa
    23341 gttacgggta gaggtggaaa ccaccgtcgc accgtctgct acacgcgcct ggttacccat
    23401 acacagggaa cagccaggga tctcgatacg agcaccactc ttaccgaaga cgctgtagta
    23461 gccttcttcg gtcagttgcg cagcgtccat acgcgttggc ggtgccaccc acaggcgggt
    23521 cggcaactga cctttgtgcg catccagcag tttaccagca gcacggaagt gaccgatgtt
    23581 agtcatgcaa gaaccgataa atacttcgtc gatcttctcg ccctgtaccg cagaaagcgg
    23641 acgtgcgtca tccgggtcgt tcggagcaca gaggattggc tctttaatat ccgccagatc
    23701 gatgtcgatc actgccgcgt attccgcatc tgcatcggct tccagcagct caggattcgc
    23761 cagccatttt tccatgccct gaatacgacg ctccagggta cgacgatcgc cgtaaccttc
    23821 cgcgatcatc cacttcagca ggacgatgtt agagttcagg tattcaatga tcggttcttt
    23881 gttcagcttg atggtacaac cggcagcaga acgctcggca gacgcatcgg tcagctcaaa
    23941 tgcctgctca actttcagat ccggcagacc ttcaatttcc agaatgcggc cagagaagat
    24001 gtttttctta cctttcttct caacggtcag cagaccttgt ttgattgcat acagcggaat
    24061 ggcatgtacc agatcgcgca gggtgatacc cggctgcatt ttgcctttga agcgtaccag
    24121 aacggattct ggcatatcca gcggcattac gcctgttgcg gctgcaaatg ccaccagacc
    24181 agaacccgcc gggaaagaga taccgatcgg gaaacgggta tgggagtcac caccggtacc
    24241 gacggtatcc ggcagcagca tacggttcag ccaggagtgg attacgccgt cacccggacg
    24301 cagcgacaca ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc
    24361 aactggcttc ggatacgccg cggtgtggca gaaagactgc atcaccaggt cagccgagaa
    24421 gcccaggcac gccaggtctt tcagttcatc acgggtcatc gggccggtgg tatcctgaga
    24481 accaacagaa gtcattttcg gttcacagta cgcgcccgga cgaatgcctt tcacgccaca
    24541 ggcacggccc accattttct gcgccagcga gaagccacgg tcgctctcag cgacatcttt
    24601 cgcctgacgg aacacatcgc tgtgcggcag gccaagtgct tcacgcgctt tggtggtcag
    24661 gccacgcccg ataatcagcg gaatacggcc gccagcacgc acttcatcaa tcagcacgtc
    24721 ggttttcagt tcgaaggttg ccagcagttc gccggtttcg tggttacgca cttcaccttt
    24781 gtacgggtaa acgtcaatta cgtcgcccat gttcaggtta gagacgtcga cttcgattgg
    24841 cagtgcaccc gcatcttcca tcgtgttaaa aaagattggt gcaattttac cgccgaggca
    24901 caaaccaccg ccgcgtttgt tcggcacatg tggaatatca tcgcccataa accacagaac
    24961 ggagttcgtg gcggatttac gcgatgaacc cgtacccaca acgtcaccga cgtacgccag
    25021 cgggaaacct ttctgttgca aagcttcaat ctgtttgatc gggccaacaa caccaggctg
    25081 atctggctca ataccttcac gggcgttttt cagcatcgcc agcgcgtgca gtgggatatc
    25141 cgggcgtgac cacgcatctg gtgccggaga gaggtcatcg gtgttggttt cgccagtgac
    25201 tttgaagacg gtaacggtca gtttttcagc cagcgccggg cgattcagga accattcggc
    25261 atccgcccag gactgcatta cctgcttcgc atattcattg cctgctttgg ctttctcttc
    25321 tacgtcatag aagttatcga acatcagcag cgtgtgagaa agtgctttgg cagcgatcgg
    25381 tgccagtttg gcatcatcca gcgcgtcgat cagcggatga atgttgtaac caccctgcat
    25441 agtgcccagc agttcaatgg ctttttctgg agtcagcagc ggggatttag cttcgccttt
    25501 ggcgacagca gccaggaagc ctgctttgac ataggcggct tcatcgacgc cagggggaac
    25561 acggttggtt aacagatcta acaggaattc ttcttcgccc gcgggcgggt ttttcagcag
    25621 ctctacaagt gcggccattt ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc
    25681 acgctcagct acgtgcttac ggtattcttc tagcacgacg gttctcctcg ctctcattgt
    25741 catagtgcgg caggcgattc tcttcacgct cctgtgagac agcagtttgt agggtaaatg
    25801 cccagtaccg caacgggcag aatagcagga ttttgacgat gtgttaatct gtttacaaaa
    25861 aagcaacata aaaaagtggc tgaatcgtta aggatggtct agagattgtt tccctccgta
    25921 aaaacttcgt taattcgcat ggtgataatc accttcctca acgaatccca agattagtca
    25981 aaatttaaac taccgcctct ttatactcgg attcacagca cctgcgggtg gcagttcgcc
    26041 gaccattgcg atttccttga gatccgaatt atgaaaatga ctttgccgtt taaaccccat
    26101 gtgctggcgc taatttgcag tgccgggctt tgtgccgcct ctgccgggct atatataaaa
    26161 agccgcacag tggaagcgcc tgtggaagcg caatcgacac aacagactgc gcctgacatc
    26221 tccgcagtta cgcttcctgc aacggtttcc gtgcctcctg taacacctgc cgtcgtcaaa
    26281 tccgcattca gcactgctca aatagatcaa tgggttgcac ctgtcgcgct ataccccgac
    26341 gccctacttt cgcaggtgct gatggcatca acctatccga caaacgttgc gcaagcagta
    26401 caatggtcgc acgataaccc acttaaacaa ggcgatgctg ctattcaggc ggtatctgac
    26461 cagccgtggg acgccagcgt taaatcactg gtggcctttc cacaattgat ggcattgatg
    26521 ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca gccgcaagac
    26581 gtgatggact cggtacagcg attgcggcaa ctggcgcaac aaaccggctc gctgaagtca
    26641 tcaacggaac aaaagatcat cacgacaacg aaaaaagtcg taccggtaaa tcagcccgcc
    26701 aacgcccctg ccacacaatc aaataccgtt tctacgtcca gccccgtcgt cgcagaacct
    26761 gcaccgaccg taatagccat tgagccagcc aatccggatg tggtttatat tccgaactac
    26821 aacccaaacg tggtttacgg cagttgggcc aataccgctt atccgccagt ttatctgccg
    26881 ccgccagccg gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta tagcatgggc
    26941 gttgctacca catacgcact attcagcagc atcgactggg acgacgacga tcatgaccat
    27001 catcatcatg acgatgatga ttatcatcac cacgatggcg gtcatcgtga cggtaatgac
    27061 tggcaacaca acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt
    27121 gagcatctta ctgataagaa tatggcatgg cggcacaatc cgaattaccg cgatggtatg
    27181 ccttatcatg atcaggatat ggcaaagcgg tttcaccaaa ccgatgttaa aggcggaatg
    27241 agcgccacgc cgctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagccag
    27301 tttcagcaac gaacacacgc cgcccccgtc attacgcgag atacccaacg tcaggcagcg
    27361 gcacagcggt ttaatgaagc tgaacacaat gggagctatg acgacttccg cgacttcagc
    27421 cgtcgccagc cactgaccca gcaacaaaag gacgccgctc gtcagcgtta tcagtcggct
    27481 tctcctgagc agcgccaggc ggctcgcgag aaaatgcaga ctaacccaca gaaccagcag
    27541 cgaagagagg cggcacgtca gcgtattcag tccgcgtcgc ctgagcagcg ccaggcggtt
    27601 cgcgagaaaa tgcagactaa cccgcagaat caacagcaaa gagatgcagc acgtcagcgt
    27661 atccagtctg cctcgcctga gcagcgtcag gtttttcgag agaaagttca ggagagccgc
    27721 ccgcagcgtc taaacgacag taaccatact gcccgattga ataatgagca acggtcagcg
    27781 gtacgcgaac ggttgtctga gcgtggagca aggagactgg aaaggtaaat tgcaggcgta
    27841 aaaaagcggc gttgttagcc gcttaagact gatgacaaac atcgaatcgc ccgatgcgca
    27901 agcttatcgg gcctacacaa gccatgcaat atattgaatt tgcatgattt tgtaggtcgg
    27961 ataaggcgtt tacgccgcat ccggcatcaa ccacgagcac tttatcaaca atctgaagcg
    28021 gccagaatag ccgcttctta atttgccgga tgttccggca aacgaaaaat tacttcttct
    28081 tcgctttcgg gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca
    28141 cagactcgtg cagagtcggg tgcgcatgga tggtcagtgc gatgtcttca gcgtcacaac
    28201 ccatttcgat tgccaggccg atttcaccca gcaactcgcc gccgttagta ccgacaatcg
    28261 caccaccgat cacacggtga gattctttgt cgaaaattag cttggtcata ccgtctgcgc
    28321 agtcggaagc gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcgtagctga
    28381 tgcctttctc tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat
    28441 aggcgatgga cgggataact ttcggatcga agtaatgttt tttaccagcg ataacttcag
    28501 cggcaacgtg accttcgtga acacctttgt gtgccagcat tggctgaccg acgatatcgc
    28561 cgatagcaaa gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac
    28621 ggtcgtcaac ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac
    28681 caatcgctac cagcacggcg tcgtaacgct gcggttcagc gggtgctttt ttgccttcca
    28741 tcgtcacata aatgccgtct tctttcgctt caacggcggt aactttggtt tccagcatca
    28801 ggttgaattt cttgctgata cgcttggtaa agactttaac gatgtcttta tcagctgccg
    28861 ggatgacctg gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtatacgg
    28921 tccccatttc cagaccgatg ataccgccac ccattaccag caggcgttct ggtacttctt
    28981 tcagttccag cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca
    29041 gttggatcgg gcgagaaccc gctgcaatga tcgcgttgtc gaagttgatc acggttttgc
    29101 cgttctcacc ttcaacttcc agggtgttag ccccggtgaa tttacccaga ccgttgacca
    29161 ctttgacttt gcggcctttt gccataccag ccagaccacc ggtcagctga ttaatcactt
    29221 tctctttcca ggtacgaatc ttgtcgatat cggttttcgg ttcgccgaag acgataccgt
    29281 gttcagccag cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag
    29341 ggatacagcc gacgttcagg caaacaccgc caagagtgtt gtaacgttct acgattacgg
    29401 tttccagacc taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa
    29461 gtaccacgac ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg
    29521 cgggtctgac gtttttttta tgacgcccat tatccaccgg gtcgttctat ccgtcggtat
    29581 tttacaaaat tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc tattcgctaa
    29641 taatctcaat acattcatga gattaccaga aaaaagccgg ccgttgggcc ggctctttta
    29701 tttacatcac cagacggcga atgtcagaca gcgtgttgtt aatgatggta atgaaacggg
    29761 caccatcagc accgtcgatc acgcggtggt cgaaggagag agaaatcggc agcatcagac
    29821 gcggcacgaa ctctttacca ttccacaccg gctccatcgc ggacttggaa acgccgagga
    29881 tagccacttc cggcgcgttc acaatcggcg cgaagtgggt agtacccagg ccgccgatgc
    29941 tggagatggt gaagcaaccg ccctgcattt cgcccgctgt cagcttaccg tcacgcgctt
    30001 tcttagaaat agtcatcagc tcgcgagaca gctcgatgat gcctttcttg ttgacgtctt
    30061 tgaataccgg gacaaccaga ccgttcgggg tatccaccgc cacaccgatg ttgatgtatt
    30121 tcttcagggt cagacgctga ccgtcttccg acagcgaact gttgaagcga ggcatctgct
    30181 caagagctgc agcaacggct ttcatgatga agacaaccgg ggtgatcttc acatccagct
    30241 tacgtttcgc cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg gtgatatcgg
    30301 ttttgtcgaa gtgagtaaca tgcgggatca tcacccagtt acggctcagg ttcgcaccag
    30361 agattttctg gatgcggccc agttccactt cttcgatttc accaaacttg ctgaagtcca
    30421 ccttcggcca cggcagcatg ccagggatac caccgccagt tgccgccgga gctgcttctg
    30481 cacgtttgat agcttctttc acgtaagcct gaacgtcttc gcgcaggata cgacctttac
    30541 ggccggtgcc cttcactttc gccaggttaa cgccaaactc gcgtgccaga cggcggatca
    30601 gcggagtcgc gtgaacgtaa gcgtcatttt cagcaaattc agatttgcct tcggcttttg
    30661 cagccggagc tgctgccggg gcttcagctt ttgctgccgg agccggcgct gccgcttcct
    30721 gtttcgcagg agctgccgca ggcgctgcgc cttcaacttc gaagatcata atcagcgaac
    30781 cagttttcac tttatcgcca acgttgactt tcagttcctt cacgacgcct gcaaacggag
    30841 ccggaacttc catagaagct ttatcgcctt ctacggtgat cagtgactgt tcagcggcaa
    30901 ctttgtcgcc cactttcacc atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg
    30961 gaacgttaac ttctttcacg ccagttgctg gtgcaggggc cgctgccgga gccgcttcct
    31021 gtttagccgc cggagctgcc gcgcctgctt cacccgcgac ttcgaagatc ataatcagcg
    31081 agccggtaga cactttgtca cccacgttca ctttgatctc tttcacggtg cctgcaaacg
    31141 gagcaggaac ttccatagaa gccttgtcgc cttctacggt gatcagcgac tgttcagctt
    31201 caactttatc gccaactttc accaggattt cggtcacttc aacttcgtcg ctgccgatat
    31261 ccggaacgtt aacgtctttt gctgccgcag ccgctggtgc tgctgccgga gctgcttctt
    31321 tcttctcttc tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca
    31381 taatcagtgc gccggtctgg gttttatcgc caacagagac tttgatctct ttaacgatac
    31441 ccgcctgcgg agacggaact tccatagagg ctttgtcgcc ttctacggtg atcagcgact
    31501 gttcggcttc aactttgtcg cccactttga ccaggatctc ggtgatttca acttcatcag
    31561 ccccgatgtc cggtactttg atttcgatag ccattattct tttacctctt acgccagacg
    31621 cgggttaact ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt
    31681 atcgatttcg ccacgtttag ccagttcgcc cagcgccgca accacgacat aagaagcatc
    31741 aacttcaaag tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc catcagtacc
    31801 cagtacgcgg tagtcgtcag ccggtacgta agtacggacc tgctcagcga acagtttcat
    31861 atagtcggta gatgccactg ccggagcgtc gttcatcacc tgagcgatgt acggaacgcg
    31921 cggagtttcc agcgggtgca gcatgttcca gcgttcacaa tcctgaccat cacgcgccag
    31981 ttcggtgaag gatgtcacgc tataaacgtc agaacctacg ccgtaatctt tcgccaggat
    32041 ctcagctgct tcacggacgt gacgcaggat agaaccggag ccgagcagct gaactttacc
    32101 tttgctacct tcaatggttt cgagtttgta gataccttta cggatacctt cctcagcacc
    32161 ttccggcatt gccggcatgt ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt
    32221 ctcttgtttt tcaccgtaca tacgctccag accgtcatgc atgatgacag caacttcgta
    32281 agcgtaagcc gggtcgtaag agatacagtt cgggatagtc agcgactgaa tgtggctgtg
    32341 accatcttcg tgctgcagac cttcaccgtt cagggtggta cgaccggaag taccgccgat
    32401 caggaagcca cgcgcttgct ggtcgccagc cgcccagcac agatcgccga tacgctggaa
    32461 gccgaacatc gagtaataga tgtagaacgg aatcatcggc agattgttgg tgctgtaaga
    32521 agtcgccgct gccagccagg aacaacctgc gcccagctcg ttgatccctt cctgcagaat
    32581 ctgacctttc tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg gggtgtactg
    32641 ctgaccgttc gggctgtaaa taccaatctg acggaacaga ccttccatac cgaacgtacg
    32701 cgcttcgtcg gcgatgatcg gtaccagacg atctttgatc gacttgttct tcagcatcac
    32761 gttcagggca cgaacgaaag cgatagtggt agagatctct ttgctctgct cttccagcag
    32821 cgcgccgaag tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg gctgacggct
    32881 tggcagataa ccgtgcagtt tctgacgctg agcgtgcaga taggtatgct cttcagaacc
    32941 ttccgggaag gtgatgtacg gcagtttttc gatatctgca tcagacaccg gcacattgaa
    33001 acggtcgcgg atatgacgca cgccgtccat gttcattttc ttaacctggt gcgcgatgtt
    33061 tttaccttca gccgcgtcgc ccatgccata acctttaatg gtatgagcaa ggattaccgt
    33121 cgctttgcct ttggtttcct gcgctttctt gaatgcagcg tagattttct tcggatcgtg
    33181 accaccacgg ttcagtgccc agatctgctc gtcagtccag tctgcaacca gtgctgcggt
    33241 ttcaggatat ttaccgaaga agtgttcgcg aacatacgca ccatctttcg atttgaaggt
    33301 ctggtaatcg ccgtcaacgg tttcgttcat cagttggatc agtttaccgc tggtatcttt
    33361 acgcagcagt tcgtcccaac ggctacccca catcactttg atcacgttcc agccagcacc
    33421 ttcgaagatg ccttccagtt cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag
    33481 acgctgcagg ttacagttga taacgaagac cagattatcc agtttttcac gggtagcgat
    33541 ggtgatcgca cctttggatt ccggttcgtc catttcaccg tcgccgagga acgcgtaaac
    33601 ggtttgttta gaggtatctt tcaggccacg gtgttccaga tatttcagga atttagcctg
    33661 gtaaatagca ccaatcggac ccagacccat agatacggtc gggaactgcc agaattccgg
    33721 catcagtttc gggtgcggat aggaagagag gccattgccg tgaacttcct gacggaagtt
    33781 atccaactgc tcctgagtca gacgaccttc caggaaagca cgagcgtaca cgcccgggga
    33841 gatgtggccc tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa
    33901 gtggttaaag cacacatcat aaatggttgc ggaagactgg aaggacgcca tgtggccgcc
    33961 cagttcgagg tcttttttcg acgcacgcag caccgtcatg atggcgttcc agcggatagc
    34021 tgaacgaata cggcgttcca gttccagatt acccggatac tccggttgtt cttcaacggg
    34081 gatggtgttg atgtagttgc tgataccggt gcctgcagct acgttgactc cgcctttgcg
    34141 ggcttcagca agcagttggt cgatcagata ctgagcacgc tcaacacctt cttcacggat
    34201 gaccgattcg atcgcctgga gccagtcgcg agtttcgatc ggatccacgt catttgggaa
    34261 acgttctgac atgggttatt ccttatctat ctaataacgt tgagttttct ggaacctgtc
    34321 ccattgaact ctcgccggaa agctcaataa gacaggttct acgtttagtt gccgcgcttt
    34381 tatatgcgct tgatttacaa catcttctgg ataattttta ccagaaaaat cactaattct
    34441 ttcgttgctc cagacgacgc agagaacgct cacggcggct ctcttcacgg cttctgtcga
    34501 gcaaaatttc ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct tcttccggct
    34561 taccggccat aatcgcttca aatatgcggg tgcggtgact actcaccagc ggcagcatct
    34621 cgcgacgcga atagagcaat tcgaagttct ggcggacatt ctgagccaac atcggctcca
    34681 tacaccttag cagatgaagc agaaccacat tgtgggccgc ttcggtgacg gcaatctgat
    34741 actggagtac ggcatttgat tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg
    34801 cgtggtggag ttcacggatg cgttccttgt cttcatcggt actacgcagc gcggcgtaat
    34861 aagcggcgat accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt gattcaggat
    34921 ggtcggagag cagctccacc agcggatcgc tgaagctttg ccataggctg ctctggacaa
    34981 aagtgccgcc accctggcga cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct
    35041 cacgcaatga gggacgggag acgtcaaact gttttgccag ttcgcgttcc ggtgggagtt
    35101 tttcgcccgg gcggagagtg ccttcgagga tcaaaaactc cagttgctgc tcaatcacat
    35161 cggagagttt tggttggcgg attttgctgt aggccatgag ttcctgtctt aagccacttg
    35221 ccgaagtcaa ttggtcttac caatttcatg tctgtgacgc taaagtaaca aagtattcac
    35281 cttatgtcca tacaggtttt gattgaaatc atgaaactgt gcacatttta acaacttgac
    35341 atatataacg tttcaaagtt gtaactatgc acaaatgtag actttacgta ggaaaggttt
    35401 tttt
//
LOCUS       DAZKHK010000042        34835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKHK010000042 DAZKHK010000000
VERSION     DAZKHK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.042"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            426..1118
                     /gene="yieP"
                     /locus_tag="ACHOS5_002043"
     CDS             426..1118
                     /gene="yieP"
                     /locus_tag="ACHOS5_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9776860.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAAKVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            1141..2568
                     /gene="mdtD"
                     /locus_tag="ACHOS5_002044"
     CDS             1141..2568
                     /gene="mdtD"
                     /locus_tag="ACHOS5_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP9776861.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYAHKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(2534..3526)
                     /gene="rbsR"
                     /locus_tag="ACHOS5_002045"
     CDS             complement(2534..3526)
                     /gene="rbsR"
                     /locus_tag="ACHOS5_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP9776862.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3530..4459)
                     /gene="rbsK"
                     /locus_tag="ACHOS5_002046"
     CDS             complement(3530..4459)
                     /gene="rbsK"
                     /locus_tag="ACHOS5_002046"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9776863.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4585..5475)
                     /gene="rbsB"
                     /locus_tag="ACHOS5_002047"
     CDS             complement(4585..5475)
                     /gene="rbsB"
                     /locus_tag="ACHOS5_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP9776864.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKVGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5500..6465)
                     /gene="rbsC"
                     /locus_tag="ACHOS5_002048"
     CDS             complement(5500..6465)
                     /gene="rbsC"
                     /locus_tag="ACHOS5_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9776865.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6470..7975)
                     /gene="rbsA"
                     /locus_tag="ACHOS5_002049"
     CDS             complement(6470..7975)
                     /gene="rbsA"
                     /locus_tag="ACHOS5_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP9776866.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDIETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7983..8402)
                     /gene="rbsD"
                     /locus_tag="ACHOS5_002050"
     CDS             complement(7983..8402)
                     /gene="rbsD"
                     /locus_tag="ACHOS5_002050"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9776867.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSATRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQQHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8569..10437)
                     /gene="kup"
                     /locus_tag="ACHOS5_002051"
     CDS             complement(8569..10437)
                     /gene="kup"
                     /locus_tag="ACHOS5_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP9776868.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10660..12156
                     /gene="ravA"
                     /locus_tag="ACHOS5_002052"
     CDS             10660..12156
                     /gene="ravA"
                     /locus_tag="ACHOS5_002052"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP9776869.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            12150..13601
                     /gene="viaA"
                     /locus_tag="ACHOS5_002053"
     CDS             12150..13601
                     /gene="viaA"
                     /locus_tag="ACHOS5_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP9776870.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKMIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13606..14598)
                     /gene="asnA"
                     /locus_tag="ACHOS5_002054"
     CDS             complement(13606..14598)
                     /gene="asnA"
                     /locus_tag="ACHOS5_002054"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP9776871.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14750..15208
                     /gene="asnC"
                     /locus_tag="ACHOS5_002055"
     CDS             14750..15208
                     /gene="asnC"
                     /locus_tag="ACHOS5_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP9776872.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15298..15741
                     /gene="mioC"
                     /locus_tag="ACHOS5_002056"
     CDS             15298..15741
                     /gene="mioC"
                     /locus_tag="ACHOS5_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP9776873.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            16120..18009
                     /gene="mnmG"
                     /locus_tag="ACHOS5_002057"
     CDS             16120..18009
                     /gene="mnmG"
                     /locus_tag="ACHOS5_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9776874.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVEKDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLELEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            18073..18696
                     /gene="rsmG"
                     /locus_tag="ACHOS5_002058"
     CDS             18073..18696
                     /gene="rsmG"
                     /locus_tag="ACHOS5_002058"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9776875.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            19313..19693
                     /gene="atpI"
                     /locus_tag="ACHOS5_002059"
     CDS             19313..19693
                     /gene="atpI"
                     /locus_tag="ACHOS5_002059"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP9776876.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            19702..20517
                     /gene="atpB"
                     /locus_tag="ACHOS5_002060"
     CDS             19702..20517
                     /gene="atpB"
                     /locus_tag="ACHOS5_002060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9776877.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            20564..20803
                     /gene="atpE"
                     /locus_tag="ACHOS5_002061"
     CDS             20564..20803
                     /gene="atpE"
                     /locus_tag="ACHOS5_002061"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP9776878.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            20865..21335
                     /gene="atpF"
                     /locus_tag="ACHOS5_002062"
     CDS             20865..21335
                     /gene="atpF"
                     /locus_tag="ACHOS5_002062"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9776879.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            21350..21883
                     /gene="atpH"
                     /locus_tag="ACHOS5_002063"
     CDS             21350..21883
                     /gene="atpH"
                     /locus_tag="ACHOS5_002063"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9776880.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            21896..23437
                     /gene="atpA"
                     /locus_tag="ACHOS5_002064"
     CDS             21896..23437
                     /gene="atpA"
                     /locus_tag="ACHOS5_002064"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9776881.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            23488..24351
                     /gene="atpG"
                     /locus_tag="ACHOS5_002065"
     CDS             23488..24351
                     /gene="atpG"
                     /locus_tag="ACHOS5_002065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9776882.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            24378..25760
                     /gene="atpD"
                     /locus_tag="ACHOS5_002066"
     CDS             24378..25760
                     /gene="atpD"
                     /locus_tag="ACHOS5_002066"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9776883.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            25781..26200
                     /gene="atpC"
                     /locus_tag="ACHOS5_002067"
     CDS             25781..26200
                     /gene="atpC"
                     /locus_tag="ACHOS5_002067"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9776884.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            26553..27923
                     /gene="glmU"
                     /locus_tag="ACHOS5_002068"
     CDS             26553..27923
                     /gene="glmU"
                     /locus_tag="ACHOS5_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9776885.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGAGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPIKKK"
     gene            28084..29913
                     /gene="glmS"
                     /locus_tag="ACHOS5_002069"
     CDS             28084..29913
                     /gene="glmS"
                     /locus_tag="ACHOS5_002069"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9776886.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVGSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            30227..31267
                     /gene="pstS"
                     /locus_tag="ACHOS5_002070"
     CDS             30227..31267
                     /gene="pstS"
                     /locus_tag="ACHOS5_002070"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9776887.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAVGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            31354..32313
                     /gene="pstC"
                     /locus_tag="ACHOS5_002071"
     CDS             31354..32313
                     /gene="pstC"
                     /locus_tag="ACHOS5_002071"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP9776888.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            32313..33203
                     /gene="pstA"
                     /locus_tag="ACHOS5_002072"
     CDS             32313..33203
                     /gene="pstA"
                     /locus_tag="ACHOS5_002072"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9776889.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            33294..34067
                     /gene="pstB"
                     /locus_tag="ACHOS5_002073"
     CDS             33294..34067
                     /gene="pstB"
                     /locus_tag="ACHOS5_002073"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9776890.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            34082..34807
                     /gene="phoU"
                     /locus_tag="ACHOS5_002074"
     CDS             34082..34807
                     /gene="phoU"
                     /locus_tag="ACHOS5_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9776891.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
BASE COUNT         8552 a         8893 c         8993 g         8397 t
ORIGIN      
        1 gagacttggt attcattttt cgtcttgcga cgtcaagaat ccgtatcttc gagtgcccac
       61 acagattgtc tgataaattg ttaaagagca gtgccgcttc gctttttctc agcggcgcgg
      121 ggtgtgcata atacgctttc ccgctaaaga gtcaagcatt tatttcgctt tctctgccga
      181 agttctcagg agaaccccgc tgacccggcg gcgtgtttgc cgttgttccg tgtcagtggt
      241 ggcgcattat agggagttat tccggcctga caagaggaaa tttaaaataa ttttctgatc
      301 gcgcattttt tcagcaaaag ccccgttaat gtgccataaa acaagcaatt tgccgttttt
      361 gcaaccgtaa tcacacttcc tggtggcata ctaagaagga ataaaaagaa aaaggaagat
      421 tagtaatgcc tttaagcgca caacagttag ctgcacagaa aaacctttcg tatgttctgg
      481 ctgagaagct ggcgcagcgg atcttaaaag gtgaatatga acccggcacc attttgcctg
      541 gtgagattga gctgggcgag caatttggag tgagtcgtac agcggtacgc gaagcggtca
      601 aaacgttaac ggcaaaaggg atggttttac cgcgaccgcg aattggtacc cgagtcatgc
      661 cacaatcgaa ctggaatttt cttgatcagg aattgcttac ctggtggatg acagaagaga
      721 actttcatca ggtcatcgat cactttctgg ttatgcgtat ctgtctggag ccgcaagctt
      781 gcctactggc agcaaaggtt ggcaccgcgg aacagaaggc gcatctcaat acgttaatgg
      841 ccgaaatggc ggcattaaaa gagaactttc gccgtgaacg ctggatcgaa gtcgatatgg
      901 cctggcatga gcatatctat gaaatgagcg ccaatccgtt tttgacctca ttcgcctcgc
      961 tattccattc ggtttatcac acttacttca cgtcaattac cagcgacaca gtgataaagc
     1021 tggatctgca ccaggcgatt gtcgatgcga ttatccaaag cgatggcgac gcggcattta
     1081 aagcttgtca ggcgctgcta cgctcaccag ataagtgata acccgataac aggacattga
     1141 atgagcgata aaaagaagcg cagtatggcg ggtttgccgt ggatcgcggc gatggccttc
     1201 ttcatgcagg cacttgatgc cactattctg aataccgcct tacccgcaat cgctcatagc
     1261 cttaatcgtt ctcctctcgc gatgcaatca gccatcatca gttatacgct gacggtggcg
     1321 atgcttattc cggtaagcgg atggctagcc gatcgcttcg gtacgcgtcg catatttacc
     1381 cttgccgtga gtctgttcac gttgggttct ctggcctgcg cactttctaa ctcgctacca
     1441 cagctggttg tcttccgggt tattcagggg ataggcggcg caatgatgat gcctgttgct
     1501 cggctggcct tactgcgcgc ttatcctcgt aatgagcttc ttcctgtatt gaattttgtc
     1561 gccatgccgg gtctggtggg gccaatttta ggccccgttc ttggcggcgt gctggtcacc
     1621 tgggcaacct ggcactggat atttttaatc aatatcccca taggtattgc gggccttctt
     1681 tacgcgcaca aacatatgcc caatttcacc accgcacgac gcagattcga taccactggc
     1741 tttttgctgt ttggcctcag ccttgttctc ttctcaagcg gaatagagct attcggggaa
     1801 aagattgtcg ccagctggat tgccttgacg gtaattgtca ccagcatcgg gttactgctt
     1861 ctctatattc tccatgcgcg acgcacgcca aacccattaa tttcattaga tttatttaaa
     1921 acccgcactt tctcgatcgg tatcgtaggc aatattgcaa cccgtctggg gaccggctgt
     1981 gtaccgttcc ttatgccatt gatgttacag gtaggatttg ggtatcaggc gtttattgct
     2041 ggctgtatga tggcaccgac agcgttaggt tccattattg caaaatcgat ggttacccaa
     2101 gtcttacgtc gtctgggcta tcgccatacg ttagtgggga tcacggtgat tatagggcta
     2161 atgatcgctc agttctcttt gcaatcacca gcaatggcga tatggatgct gatcttgccg
     2221 ttgtttatat tagggatggc tatgtcgaca caatttaccg cgatgaatac catcacactt
     2281 gccgatctga ccgatgacaa cgccagcagc ggtaacagtg ttctggcggt cacgcagcaa
     2341 ctgtcgatca gtttaggcgt tgctgtaagt gcggcagtcc ttcgcgttta cgaagggatg
     2401 gaaggcacaa cgacagtcga acaattccac tatacgttta tcacgatggg cattattact
     2461 gttgcttcag cagcaatgtt catgctactg aaaacaaccg atggtaataa tttgatcaaa
     2521 agacagcgta aatctaagcc gaaccacgtt ccatcagaat cggagtaagt tgtaatcgtt
     2581 gttgctgtag ggtcggctgg gttatccgat ggatgagtac atcaatcgcc agctccccca
     2641 gttcatcttt cggttggtgt atagttgtta atggtggcgt cataaagctt gccagttcga
     2701 tatcgtcata gccaatcacc gcgatatcct gcggaacctg taactctgcc tgatataacg
     2761 cctggtaaac gccaacagcc atagcgtcat ttccggtaaa gacggcctga ggacgcagcg
     2821 gatgtgataa cagttggcgc atagcgtcaa acccgccgtt aaattcaaaa tcaccagtga
     2881 cttcatagcc atcaggaatg ttgagacccg cacgtttcat cgcagcccga taaccttcca
     2941 accgcaggcg cgccggagtt ttatccagcg gaccggtaat acaggcaata cgggtatgac
     3001 ctttatcgat cagatattgc gttgctaagt ctccgcccag caacgagtta tcctgaataa
     3061 gatcgctgtc gccatcgaac ggagcccagt ccatcatcac ggtaggcact gtcggataac
     3121 gttgcatgat ttcacgcgaa ggctgatgcg tttcggtgca cagtaacagc aagccatcaa
     3181 cgcgtttttg catcagcgtt tccagattac gattcatccg ctgttcatcg ccttcggtat
     3241 tgcaaaggac aagactataa ccgcgttcga agcagctgcg ttcaacgcca cgcaccagtt
     3301 ctgaatagaa aggattggta ctggcagtga tcaacatgcc aatggtatgt gtttgattga
     3361 gtttgaggct acgcgccaga gctgatggcg cgtaattgag ttctttaatc gccgcttcaa
     3421 ctttggctgt aatcgcttca ctgacgaagc gatctttatt gataacgtga gaaactgttg
     3481 aggtagaaac gcccgccagg cgggcaacat ctttcattgt agccaagcgt cacctctgcc
     3541 tgtctaaaaa tgcgtcgatc tcttcacgcc acggtacgga aggttgtgcg cctttacgtg
     3601 ttacggcaat cgcagcggct gcatgggcaa aacgaatcgc ctctggcaat ggtttttctt
     3661 ccagcaacgc cgtgattaac gcgccgttaa aggtatctcc ggcagcaata gtatcgacag
     3721 cctgcacccg gaatccagga acgcgctgac cttcaccatt cacgctagcc catacaccac
     3781 gacttcctaa agtaatcagt acagtacgga tacctttttc atgcagtacc tgcgccgcct
     3841 tcgctgcatc ttcatcattt tcaacacgaa taccggtgag cttttctgct tccgtttcgt
     3901 ttggcgtaat aatgtccacc agcgccagca gttcgtcagg aagttcgcga gccggagccg
     3961 ggttaagcgc aacgatagtc ttattttgat gggcgatttt cgccgctgcc atcacacttt
     4021 cgagtggtga ttccagctgc attaataatg ctgacgcgtt ggcaatacgc tcacgttgcg
     4081 cttccaccag cgccggggaa agggcagcat tagcgccggc atgaataccg atgacattct
     4141 caccttcgcc attaacaaaa atcagcgcca cacctgttga ttcgcctttg atcacgctga
     4201 ccggagaaat atcgatgtta tcagtggcga gctgctggcg aacgctctca ccaatgctgt
     4261 catcgccagt gcaggcaata aacgcgatat tcgcaccgct acgcccagca gccaccgcct
     4321 gattcgcgcc tttgccgcca aatgcaacct gatagtggtt accggttacg gtttcgcctg
     4381 gagtaggaaa agattgaaga ttaagaatgt ggtcagcatt aatgctgcca agaacaacga
     4441 ggctgcctgc gttttgcata ttcgggatgt ccataaaatg cgccaccgtg ttagggtggc
     4501 gcgtgccctg cttttcttta tattacacgt tcatctgtcg atgacgtatt tatgtcacca
     4561 tcaggtcata caacctgatt aaaactactg cttaacaacc agtttcagat caaccggata
     4621 cttagcctga actttctcgc ctttcagcac tttatctgcg gtttcgacgc ctttcgcgcc
     4681 aatctgatcg ggtagctgag cgatagtcgc tgctagtttg ccatcattca ccgctttttc
     4741 gccatccggt gtaccgtcaa atccgacgac catcacatcc gatttaccgg cagtttgcag
     4801 tgcgcgcagc gcgccaagcg ccatttcatc attctgcgcg aatacagcct gaacatccgg
     4861 atgagcggtc aacaggttct gcattacgtt caaacctttg gtgcgatcaa aatctgcagg
     4921 ctggctggca agaacattaa acttgtgagc agcaacggcc tgctggaaac cttcgccacg
     4981 ttcacgggct gcggatgtac cggcaatgcc ttgcagctcg ataactttgg caccttcacc
     5041 cactttcttc gcgatgtaat caccggcgat tttgccgccc agtacgttat cagatgcaat
     5101 gtggctcacc acttcacctt tcgttgcctg gcggtcaaga gtgataaccg ggatgttcgc
     5161 ctggttagcc atcttcacag cattacctac tgcgtcggag tcggtcgggt taatcagcag
     5221 aattttggtg ccgcggacgg ttaagtcctg cacgttcgcc agctctttcg ccgggttgtt
     5281 ctgggagtcc agcaccacca ggttatagcc aagtttatcc gcctctttct gcgcgccatc
     5341 tttcagcgat acaaagaacg ggttgttaag cgtggagacc accagcgcga tggtgtcttt
     5401 tgccatcgca ttcgcactga cggtggcgct tagcgcaaca gcggaaacca gggtagccag
     5461 ttttttcatg ttcatattca agatgtcctg tagtcgttat tactgctttt tgttgtctac
     5521 cagcaccgcc agcaaaatca ccaccgcttt gacgatcatc tggtaatagg aggaaacacc
     5581 taacaaattc aatccattat taaggaagcc aagaattaat gcgccgatca acgtcccaac
     5641 aatgcgacct tttccgcccg ccagactcgt accgcccaga accaccgcag caatagcatc
     5701 cagctcatag ccagtccccg ccgtgggttg tgcggaggag agacgcgcca cttcaatgat
     5761 cccggccagc gatgccagca gaccacaaag agaatagacg atgattttga ttttattgac
     5821 gttgatacca gaaagacgcg ttgccgcttc attgccgccc agcgcgtaga tgtaacgccc
     5881 cagacgcgtg tgatgcagca tgtaccaggc cgcgaggaag acaatcccca tgatccagac
     5941 tggcgtcggt acgcccagcg gacgaccaat accaaaccag ccaaacagat cggcgttctc
     6001 agtaaagccg gtattcactg ggctaccatt ggtataaacc atggtcacgc cgcgcagtaa
     6061 aagcatcata accagcgtag cgataaacgc ctggacgcga cctttcgcta caatcacccc
     6121 agttaccgca ccaattgcgg cacctaacgc gagagcggca gcgacagcca ccagcgcatt
     6181 gacttcaatg ccgacgatag atgcagcaac tgcgccggtc agcgccaaca gagaacctac
     6241 cgacaagtcg atacccgacg tcaggatcac cagcgtcatc ccgaccgcca taatggcgtt
     6301 caccgaggtt tgctgaagaa tattgaataa gttattgatg gtgaaaaagt tcgggcttaa
     6361 cgtcgagaca atcgcgatca gcaccagcag agcgataagc gatttctgct ccatcagcca
     6421 cgctttcgtg aaataacggc gaccagagac agtctgggtt gtcatttttt tactcctgat
     6481 tcacgcgatt aagcttgccc acagcggcag ccattaacac ttcctgggtg gcctgctcac
     6541 gagtaaattc cccgctgaga tgcccttcat gcatgacgat gatgcgatcg ctcatgccta
     6601 atacttctgg catctccgat gacaccagaa tgatgctcaa gccatcggct ttgaactggt
     6661 taatcagttg atagatctct tttttcgcgc cgacatctac gccacgggta ggctcatcga
     6721 ggatcaacac tttagggcgt gtcatcagac cacgggcaat cgctactttt tgctgattgc
     6781 caccggaaag cagaccaatt gcctgttcca tcgatggcgt tttcacatta aacagacgaa
     6841 tgaaatcact cacagcctgc tgttcatcgg catgcttcaa actgccgcca gcgcggctga
     6901 agtagcgcag cgcggtcagc gacatgttct cttttactga catgcccagc actaaaccgt
     6961 cacgtttacg gtcttcggag atataaacaa tgccgtttgc caggccatcc tgcggcgaac
     7021 gggtaacgac ttcatgccca tccagggtga cgtaaccgct tgtgcgcggc agtgcaccgt
     7081 agagcacttt catcagttcg gtacgcccag cgcccatcaa accagagacg ccaagaattt
     7141 cgcctttgcg taaagtaaaa gagacatcgt taacgccagg tccgcagaga ttatcgactt
     7201 tcaggcggat atctcccggc gctttgtcca ggtgcggata ttgatcttcc agcttgcggc
     7261 ccaccatcat ctcaatcaga gaatcttcgg tcagtgatgc cacttcgcgc tcagcaataa
     7321 attgtccgtc acgaaaaacg gtaacgtcat cgcaaatctc gaagatttct ttcatgcggt
     7381 gggagatata gacaataccg cggccttgcg atttcagctc gcggatgacg cggaacaagg
     7441 attcggtttc gatatcggtc agcgcatcgg tcggttcatc cataatgatg actttcgact
     7501 caaaactcag cactttggcg atttcaacca tttgctggtc accgatggaa agatcgccca
     7561 ccagcttgtc gcttttaaag cgcaggttaa gtttagccag caatttatcc gcttcggcat
     7621 acatggtttt ccagtcaatt ttgccaaagc gattaacaaa ctcacgaccg aggaaaatgt
     7681 tttcggcaat ggttaactgc gggatcaggt tcagttcctg gtggataatc ccaatcccgg
     7741 cttcctggga agatttcggc ccggtaaatg tcgtttcttt ccccaaccat aaaagcgtac
     7801 cggcatcgcg agtatagata ccagtaagca ctttcatcat ggtggattta cccgcgccgt
     7861 tttcgcccac cagcgccatc acgcggcccg gatagacatt taacgctgcg cccgagaggg
     7921 cttttacgcc cgggaaggct ttatcgatgc ctttaagctg aagtaatgct tccatgacgg
     7981 cctcagaacg tcacgccagc acagagaatg atattcgcat acggagaaca ttctccgctg
     8041 cgaattaccg cctgactttc tgcggtttgt tgtttgaatt gttcatgcgt ggtgtaacga
     8101 atctcaatgg tgtttccctg gtgttgttgc agctgctcaa ggtgagtgag caacgtttcg
     8161 tggagttgcg gattatgttg tttgatctct tccgcgataa tgacagcctc gacctgcatt
     8221 tcatttgtga cgacgcccag cacctgcata aaagaaggta caccctgggt taatgccata
     8281 tcgatacgcg ttgcactttt ggggatgggt aaaccagcat cacacaccac cagcgtatcg
     8341 gtatgtccca gtcgggagat caccgatgaa atatcagaat taagaacggt gccttttttc
     8401 atttttttct ccatcagcga aacgtttcgc tgacccgcag tttaatcctg actgagttgg
     8461 gaaaaccatc gtgacgttac ggaaatgtga tcaacctcga aacgttttac atggtgatta
     8521 accatgaaaa caaaaacgcc cccttatgga gaaaggaggc gtctggcgtt agatttcgac
     8581 ctgagtaccc agttcgataa ctctgtttgg cgggatttca aattgatccg gtgcccgcag
     8641 cgcattacgt tgcagcagca agtacagctt gccgcgcaga cgcaaatacc acgggcgttt
     8701 gccgaggatc aacgactcat gcgacataaa gaaggaggtt tccatcatcc ggcaacttaa
     8761 tccttccaga ccgcagcggt ggaaaacttc ttccacgttt ggcgtttctc gccaaccata
     8821 acttgccacc acgcgccaga aagtgggcga cagttgttca atctgtaccc gacggacgtt
     8881 atggacatat ggcgcgtctt cggtgcgcag agttaacaga atcacccgct catgcaatac
     8941 cttgttatgt ttcaggttat gcatcagcgc aaagggaatg acgttgattg cacgcgacat
     9001 atacaccgcg gtcccaggca cgcgaacggg cggtgatttc tccagcgaag caatcatcgc
     9061 ttccagagag ttaccatgtt catgcatccg ccgcagcaag cggaaacgct cgctcttcca
     9121 ggtggtcatc acgataaaca tcaccgtacc gaggctcaat ggcaaccagc cgccggagag
     9181 cagtttatcg aggttagcgg tgaacaaggg aatatcgaca caaaggaaag caatcaggat
     9241 cagcgcaaca aaatacttat tccagtgcca gttctgacgt gccacggtag tcgagagaat
     9301 agacgtcagc accatggttc cggtcaccgc aatcccgtac gccgccgcca ggttgctgga
     9361 gtgctcaaag ctgacaatca caatcacgac cgcgacatag agcatccagt tcacaaaggg
     9421 aatatagatt tgccctgact ccatttcgga agtgtgaata atgcgcatcg gcgacaaata
     9481 tcccagacgt accgcctgac gcgtcaatga gaagacgcca gagataaccg cctgcgaggc
     9541 aattaccgtc gccagtgcgg cgatgatcag cagcgggatc agcgcccagt ccggtgccaa
     9601 caggaagaac gggttcttaa tcgcttccgg attctttaac aacagcgctc cctggccgaa
     9661 gtaattaagg gttaaggaag gcaatacgac ggtgaaccac gccagacgaa tagggaactt
     9721 accaaagtgc cccatatcag catacagcgc ctcgaccccc gtaatcgaca gcaccactgc
     9781 ccctaatgca ataaaagaaa ccgttttgta ttcaaggaag aaatgcaccg cccacatcgg
     9841 attcagtgca tgcagcactt ccgggttagc aataatgcta cgtaacccca gccctgccag
     9901 aatcaaaaac caggtcagca tgatcggcgc aaacagctta ccgaccatag ccgtgccatg
     9961 tttttgaatc atgaataata atgtgagaac gataattgag aggggaacta tccaggtatc
    10021 cagctgcggg gcgacgattt ccagaccttc aatggcggac atcaccgaaa tagcgggagt
    10081 aatgacgact tcaccataga aaaagctgcc gccgattagc cccataatca ccagcattga
    10141 tgtggttcgc gccgacgtat tacgcccggc aagcgacatc aacgtcagga tccccccttc
    10201 accggcgtta tctgcccgca tcacgaaggt gagatattta atggaaacca caaagattag
    10261 cagccagaag atcagcgata aaaagccaaa cacggcatcg cgttcaacgc caaaaccaaa
    10321 ctggccggac aaacattcac gaagtgtata taacgggctg gtaccaatat cgccgtagac
    10381 aactccaatc gccgcgaggg taatcgcggg caatgattgc ttattatcag tgctcataga
    10441 ctagtctttc gttgaaatat gaaatgtgtg cttagtccct tggcccacaa aaaagcgcac
    10501 agtatgcacg attaacggca aaatcgtact cctaaatgcg gccacattaa cctggctcaa
    10561 agaaaaatac ccctcctttg agactatttt agccaccagc aagaaacgtc tatactcgca
    10621 atttacgcag aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta
    10681 gcggaaagaa tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac
    10741 gccatccgct tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggccca
    10801 ccaggtattg ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc
    10861 gcgtttgaat atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct
    10921 attcaggcgc taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa
    10981 gctgaaatcg tctttctgga tgagatctgg aaagcgggtc cggcaattct taataccctg
    11041 ctcaccgcca ttaacgagcg ccagttccgc aacggcgcac tcgtagaaaa aatcccgatg
    11101 cgtctgctgg tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta
    11161 tatgaccgca tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc
    11221 tccatgctga ccagtcaaca ggatgaaaat gacaatccgg ttcctgcctc cttgcagatc
    11281 acagatgaag aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat
    11341 gtatttgagc tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat
    11401 gtctcggatc gtcgctggaa aaaagcgatc cgcttattgc aggccagcgc cttttttagc
    11461 ggtcgcagtg ctgtcgcccc ggttgatctc attttgctga aagattgcct gtggtatgac
    11521 gcgcaaagcc tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcacgcctgg
    11581 caacagcaag ggatgttgac ccgcctgggc gcgattgtgc aacgtcacct gcaacttcag
    11641 cagcaacaaa gcgataaaac agccttaacg gtaattcgtc tgggcggcat ttttagccgt
    11701 cgtcagcagt atcaactccc tgttaacgtt actgcttcca ctctgactct gctgctgcaa
    11761 aaaccgttaa aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg
    11821 gaacagtggc tgagtaaagg tggtgagatt cgcggcaaac tgaacggtat cggctttgcc
    11881 cagaaactga atctggaagt cgatagcgcc caacatcttg ttgtacgcga tgtaagttta
    11941 caaggcagta cgctagcact tccaggttca tcggctgaag gtctgccagg tgaaataaaa
    12001 caacaactgg aagagcttga aagcgactgg cgcaagcaac acgctttatt cagcgaacag
    12061 caaaaatgtc tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat
    12121 gtcggcgcac agattcgcca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc
    12181 tggccgtcag cgaagaggga ttgatcgaag agatgatcat cgcgctgctg gcctcaccgc
    12241 agctggcagt cttctttgaa aaattcccac gactgaaggc ggcaatcact gatgatgttc
    12301 cccgctggcg tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca
    12361 ccgaagaggt gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc
    12421 agctaccaca aatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc
    12481 gccagttggt tgatgctaac agcacgatca cttcagcgtt acacacgctt tttctccagc
    12541 gctggcgttt aagcctgatc gtgcaagcaa cgacgttaaa tcagcagcta ttagaagaag
    12601 aacgcgaaca actgttgagt gaagttcagg aacgcatgac gctgagcggg caacttgaac
    12661 cgattctcgc agataacaat accgcagctg gtcgtctgtg ggatatgagc gccggtcagc
    12721 ttaaacgtgg cgactatcag ttgattgtga aatacggtga atttcttaac gaacagccgg
    12781 aactgaaacg tctggcagaa cagttggggc gttcccggga agccaaatca ataccgcgca
    12841 acgatgcgca gatggaaacc ttccgcacca tggtgcgcga accggcgaca gttcctgagc
    12901 aggttgatgg tctgcagcaa agcgatgata ttttacgtct cttgccgcca gaactggcga
    12961 cactagggat aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc
    13021 tcacctatcg cctgcatggt gagtcgtggc gtgaaaaaat gatcgaacgc ccggtggtgc
    13081 ataaagatta cgacgaacag ccgcgcggac cgtttattgt ctgcgtggat acttccggct
    13141 caatgggcgg ctttaatgaa cagtgtgcga aagcgttctg cctggccttg atgcgcatag
    13201 ccctcgctga aaaccggcgc tgctatatta tgctattttc caccgagatc gtccgttatg
    13261 agctttcagg cccacaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg
    13321 gcggcaccga tctggccagt tgttttcgcg ccattatgga acgcttgcaa agccgggaat
    13381 ggtttgatgc cgatgcggtg gtgatttctg attttatcgc ccagcggttg cctgacgacg
    13441 tgacgagcaa agtgaaagag ttgcagcggg tacatcagca tcgctttcat gccgtggcga
    13501 tgtcggcaca tggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata
    13561 ccgggatgcg aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg
    13621 gacgctctcg cgaacagctg ccggccatac accacactga acctggccga tatgtggcag
    13681 ttgcagcagc agcatggtca aacgagactg cccgataccg ccgccgatgg tctgcggcat
    13741 ttcaccgcgc agcagcgcct gatgccactc cagctgcagg cgatcttcgt caccggtcag
    13801 cgccagctga tgcttcagcg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa
    13861 cgcatcttcc agtaccgggt tccataccag aatatcgccg ttcagacccg catggcccag
    13921 ctctgacggg gtgctccagt catcataatc cggtgcgcgc acgtcgtggc gatgaccatc
    13981 actcaacttg ccgccaatcc cgacgaggaa taccgcgcca agatctttcg caatcgctcg
    14041 ctcacgccct ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa
    14101 gtggatctga tccggcagga acggtgccag gccaaactct tcgctaaccg cagcttcggt
    14161 tgctttaatt cccgcccaga tcgcctctac cgtgcttttc agagtcgaga attgacgctc
    14221 gccgtcgccc attacgcgtt cccagtccca ctggtctaca tagaccgagt gcaacgggga
    14281 aagacggtct tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct
    14341 gaagtcgtgt tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa
    14401 ctgggcatca ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt
    14461 atcctgcgtg ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag
    14521 acgttcttcc agttgacgag aaaagtgaga tttcacgaag ctaatttgac gttgtttggc
    14581 aatgtaagcg gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa
    14641 aaaccaacac acatgcaata atcattcaat aaaaatcctc acgtactttt gattcattaa
    14701 aaaagaaggc taaaatagaa tgaatcatca atccgcataa gaaaatccta tggaaaatta
    14761 tctgatcgac aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc
    14821 ttacgccgaa ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga
    14881 gaaaatgaag caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct
    14941 tggttatgac gtaggctgct ttatcggcat tatattaaag agcgccaaag actatccttc
    15001 cgcgctggca aagctggaaa gcctcgatga agtcactgaa gcctattaca caaccggcca
    15061 ctacagcatc tttataaaag tgatgtgccg ttcgatcgat gctctccagc atgtacttat
    15121 caacaagatc caaacaattg atgaaattca gtccaccgag acactgatcg tcctgcagaa
    15181 cccgatcatg cgtaccatta agccctgatc ggctttttta atcccatact tttccacagg
    15241 tagatcccaa cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg
    15301 gcagatatca ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac
    15361 ctggctgaaa agctggaaga ggcgggtttt accaccgaaa cgctgcacgg tccgctgttg
    15421 gaagatttat ctgcctcggg gatctggctg gttatcagct caacccacgg tgccggagat
    15481 attccggaca acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct
    15541 gcggtccgct ttggcgcaat cggtattggt agtcgtgaat atgacacctt ttgtggggct
    15601 atcgataaaa tcgaggcaga actcaaaaat tccggagcaa aacagacagg cgaaacactg
    15661 aagatcaaca ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg
    15721 tggattaatt tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca
    15781 ggaagcttgg atcaaccggt agttatccaa agaacaaccg ttgttcagtt tttgagttgt
    15841 gtataacccc tcattctgat cccagcttat actgtccagg atcaccgatc attcacagtt
    15901 aatgatcctt tccaggttgt tgatcttaaa agccggatcc ttgttatcca cagggcagtg
    15961 cgatcctaat aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat
    16021 acccaggatc ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct
    16081 tcaatccatt ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt
    16141 gacgtcatca tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc cgcggcgcgt
    16201 atgggtcaac agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc
    16261 aacccggcga tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc
    16321 ggtctgatgg cgaaagcgat cgatcaggcg ggtatccagt ttaggatact aaacgcaagc
    16381 aagggaccgg cggttcgcgc tacccgagct caggcagatc gtgtgctcta ccgtcaggcg
    16441 gtacgtacgg cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat
    16501 cttattgtcg aaaaagatcg cgtggtcggt gccgttaccc aaatgggact gaagttccgt
    16561 gccaaagctg tcgtgctcac cgttgggacg ttcctcgacg gtaaaattca tatcggtctg
    16621 gataattaca gcggtggccg tgctggtgat ccgccgtcca ttccgctttc tcgccgtttg
    16681 cgtgaactgc cgctgcgcgt tggtcgtctg aaaaccggga caccaccgcg tattgatgct
    16741 cgaaccatcg actttagcgt actggcgcaa cagcatggcg ataacccaat gccggtattc
    16801 tcgtttatgg gcaatgcgtc ccagcatccc cagcaggtgc cgtgttatat cactcatacc
    16861 aacgagaaaa cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtacgcaggg
    16921 gtgatcgaag gtgtcggccc acgctactgc ccgtcgatcg aagacaaagt catgcgcttc
    16981 gccgacagaa atcagcatca gatcttcctt gaactggaag ggctgacctc taacgaaatt
    17041 tatccgaacg gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctcc
    17101 atgcagggga tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc
    17161 ttcgatccac gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt
    17221 gctggtcaga ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggct
    17281 ggtcttaacg ctgcccgtct gtctgctgac aaagaaggtt gggctccggc gcgttctcag
    17341 gcatatctcg gcgtactggt tgatgacctg tgcactttag gaaccaaaga accgtatcgt
    17401 atgtttacct cgcgcgcaga atatcgtctg atgctgcgcg aagataatgc tgatctgcgt
    17461 ttgactgaaa tcggtcgcga actgggcctg gtggatgacg aacgttgggc gcgctttaac
    17521 gagaaacttg agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg
    17581 tcggcggaag ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc
    17641 agtggtgaag atctgctacg tcgtcctgaa atgacttatg aaaaattaac cacgctgaca
    17701 ccgtttgccc ctgcgttgac agatgaacag gctgcggaac aggttgagat tcaggttaaa
    17761 tacgaaggtt atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaat
    17821 accctgctac cagcgacgct ggattaccgc caggtatccg gtctttctaa cgaagtgatc
    17881 gccaaactta acgatcacaa accggcctct attggccagg cttcgcgtat ttctggcgtc
    17941 acgcctgcgg ctatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt
    18001 agcgcataac gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa
    18061 caggtaatca ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt
    18121 accgatcacc agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaataaa
    18181 gcgtacaacc tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat
    18241 agcattgtgg tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggaccg
    18301 ggactgcctg gcattccact ctctatcgtg cgtcctgaag ctcatttcac gttgttggat
    18361 agccttggta aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat
    18421 attgagccag tacagagcag ggtagaagag tttccgtcag agccgccatt tgatggcgta
    18481 attagccgcg cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt
    18541 gagcaaggcc gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg
    18601 cccgaagaat atcaggtcga atcagtggtt aaacttcagg ttccagccct ggatggcgaa
    18661 cgtcatctgg tggtgattaa agcaaataaa atttaatttt tatcaaaaaa atcataaaaa
    18721 attgaccggt tagactgtta acaacaacca ggttttctac tgatataact ggttacattt
    18781 aacgccacgt tcactctttt gtatcaacaa gataacgtgg ctttttttgg taagcagaaa
    18841 ataagtcata agtgaaaata tcagtctgct aaaaatcggc gctaagaatc atcattgact
    18901 gttaaaacat tattaaaaat gtcaatgggt ggtttttgtt atgtaaatgt catttattaa
    18961 aacagtatct gtttttagac tgaaatatca taaacttgca aaggcatcat ttgccaagta
    19021 aataaatatg ctgtgcgcga acatgcgcaa tatgtgatct gaagcacgct ttatcaccag
    19081 tgtttacgcg ttatttacag tttttcatga tcgaacaggg tttgcagaaa agtcgcaatt
    19141 gtatgcactg gaaaaatatt taaacattta ttcacctttt ggctacttat tgtttgaaat
    19201 cacgggggcg caccgtataa tttgaccgct ttttgatgct tgactctaag ccttaaagaa
    19261 agttttatac gacacgcggc atacctcgaa gggagcagga gtgaaaaacg tgatgtctgt
    19321 gtcgctcgtg agtcgaaacg ttgctcggaa gcttctgctc gttcagttac tggtggtgat
    19381 agcaagtgga ttgctgttca gcctcaaaga ccccttctgg ggcgtctctg caataagcgg
    19441 gggcctggca gtctttctgc ctaacgtttt gtttatgata tttgcctggc gtcaccaggc
    19501 gcatacacca gcgaaaggcc gggtggcctg gacattcgca tttggcgaag ctttcaaagt
    19561 tctggcgatg ttggtgttac tggtggtggc gttggcggtt ttaaaggcgg tattcttgcc
    19621 gctgatcgtt acgtgggttt tggtgctggt ggttcagata ctggcaccgg ctgtaattaa
    19681 caacaaaggg taaaaggcat catggcttca gaaaatatga cgccgcagga ttacatagga
    19741 caccacctga ataaccttca gctggacctg cgtacattct cgctggtgga tccacataac
    19801 cccccagcca ccttctggac aatcaatatt gactccatgt tcttctcggt ggtgctgggt
    19861 ctgttgttcc tggttttatt ccgtagtgta gccaaaaagg caaccagcgg cgtgccaggt
    19921 aagtttcaga ccgcgattga gctggtgatc ggctttgtta atggtagcgt gaaagacatg
    19981 taccatggca aaagcaagct gattgctccg ctggccctga cgatcttcgt ctgggtattc
    20041 ctgatgaacc tgatggattt actgcctatc gacctgctgc cgtacattgc tgaacatgta
    20101 ctgggtctgc ctgcactgcg tgtggttccg tctgctgacg tgaacgtaac gctgtctatg
    20161 gcactgggcg tatttatcct gattctgttc tacagcatca aaatgaaagg catcggcggc
    20221 ttcacgaaag agttgacgct gcagccgttc aatcactggg cgttcattcc tgtcaactta
    20281 atccttgaag gggtaagcct gctgtccaaa ccagtttcac tcggtttgcg actgttcggt
    20341 aacatgtatg ccggtgagct gattttcatt ctgattgctg gtctgttgcc gtggtggtca
    20401 cagtggatcc tgaatgtgcc gtgggccatt ttccacatcc tgatcattac gctgcaagcc
    20461 ttcatcttca tggttctgac gatcgtctat ctgtcgatgg cgtctgaaga acattaattt
    20521 accaacacta ctacgtttta actgaaacaa actggagact gtcatggaaa acctgaatat
    20581 ggatctgctg tacatggctg ccgctgtgat gatgggtctg gcggcaatcg gtgctgcgat
    20641 cggtatcggc atcctcgggg gtaaattcct ggaaggcgca gcgcgtcaac ctgatctgat
    20701 tcctctgctg cgtactcagt tctttatcgt tatgggtctg gtggatgcta tcccgatgat
    20761 cgctgtaggt ctgggtctgt acgtgatgtt cgctgtcgcg tagtaagcgt tgcttttatt
    20821 taaagagcaa tatcagaacg ttaactaaat agaggcattg tgctgtgaat cttaacgcaa
    20881 caatcctcgg ccaggccatc gcgtttgtcc tgttcgttct gttctgcatg aagtacgtat
    20941 ggccgccatt aatggcagcc atcgaaaaac gtcaaaaaga aattgctgac ggccttgctt
    21001 ccgcagaacg agcacataag gaccttgacc ttgcaaaggc cagcgcgacc gaccagctga
    21061 aaaaagcgaa agcggaagcc caggtaatca tcgagcaggc gaacaaacgc cgctcgcaga
    21121 ttctggacga agcgaaagct gaggcagaac aggaacgtac taaaatcgtg gcccaggcgc
    21181 aggcggaaat tgaagccgag cgtaaacgtg cccgtgaaga gctgcgtaag caagttgcta
    21241 tcctggctgt tgctggcgcc gagaagatca tcgaacgttc cgtggatgaa gctgctaaca
    21301 gcgacatcgt ggataaactt gtcgctgaac tgtaaggagg gaggggctga tgtctgaatt
    21361 tattacggta gctcgcccct acgccaaagc agcttttgac tttgccgtcg aacaccaaag
    21421 tgtagaacgc tggcaggaca tgctggcgtt tgccgccgag gtaaccaaaa acgaacaaat
    21481 ggcagagctt ctctctggcg cgcttgcgcc agaaacgctc gccgagtcgt ttatcgcagt
    21541 ttgtggtgag caactggacg aaaacggtca gaacctgatt cgggtaatgg ctgaaaatgg
    21601 tcgtcttaac gcgctcccgg atgttctgga gcagtttatt cacctgcgtg ccgtgagtga
    21661 ggctaccgct gaggtagacg tcatttccgc tgccgcactg agtgaacaac agctcgcgaa
    21721 aatttctgct gcgatggaaa aacgtctgtc acgcaaagtt aagctgaatt gcaaaatcga
    21781 taagtctgta atggcaggcg ttatcatccg agcgggtgat atggtcattg atggcagcgt
    21841 acgcggtcgt cttgagcgcc ttgcagacgt cttgcagtct taaggggact ggagcatgca
    21901 actgaattcc accgaaatca gcgaactgat caagcagcgc attgctcagt tcaatgttgt
    21961 gagtgaagct cacaacgaag gtactattgt ttctgtaagt gacggtgtta tccgcattca
    22021 cggcctggcc gattgtatgc agggtgaaat gatctccctg ccgggtaacc gttacgctat
    22081 cgcactgaac ctcgagcgcg actctgtagg tgcggttgtt atgggtccgt acgctgacct
    22141 tgccgaaggc atgaaagtta agtgtactgg ccgtatcctg gaagttccgg ttggccgtgg
    22201 cctgctgggc cgtgtggtta acactctggg tgcaccaatc gacggtaaag gtccgctgga
    22261 tcacgacggc ttctctgctg tagaagcaat cgctccgggc gttatcgaac gtcagtccgt
    22321 agatcagccg gtacagaccg gttataaagc cgttgactcc atgatcccaa tcggtcgtgg
    22381 tcagcgtgaa ttgatcatcg gtgaccgtca gaccggtaaa accgcactgg ctatcgatgc
    22441 catcatcaac cagcgcgatt ccggtatcaa atgtatctat gtcgctatcg gccagaaagc
    22501 gtccaccatt tctaacgtgg tacgtaaact ggaagagcac ggcgcactgg ctaacaccat
    22561 cgttgtggta gcaaccgcgt ctgaatccgc agcactgcaa tacctggcac cgtacgctgg
    22621 ttgcgcaatg ggtgaatact tccgtgaccg cggtgaagat gcgctgatca tttacgatga
    22681 cctgtctaaa caggctgttg cttaccgtca gatctccctg ctgctccgtc gtccgccagg
    22741 acgtgaagca ttcccaggcg acgtattcta cctccactct cgtctgctgg agcgtgctgc
    22801 gcgtgttaac gccgaatacg ttgaagcttt caccaaaggt gaagtgaaag ggaaaaccgg
    22861 ttctctgact gcgctgccga ttatcgaaac tcaggcgggt gacgtttctg cgttcgttcc
    22921 gaccaacgta atctccatta ccgatggtca gatcttcctg gaaaccaacc tgttcaacgc
    22981 cggtattcgt cctgcggtta acccgggtat ttccgtatcc cgtgttggtg gtgcagcaca
    23041 gaccaagatc atgaaaaaac tgtccggtgg tatccgtacc gctctggcac agtatcgtga
    23101 actggcagcg ttctctcagt ttgcatccga ccttgacgat gcaacacgta agcagcttga
    23161 ccacggtcag aaagtgaccg aactgctgaa gcagaaacag tatgcgccga tgtctgttgc
    23221 gcaacagtct ctggttctgt tcgcagcaga acgtggttac ctggcggatg ttgaactgtc
    23281 gaaaatcggc agcttcgaag ccgctctgct ggcttacgtc gaccgtgatc acgctccgtt
    23341 gatgcaagag atcaaccaga ccggtggcta caacgacgaa atcgaaggca agctgaaagg
    23401 catcctcgat tccttcaaag caacccaatc ctggtaacgt ctggcggctt gccttagggc
    23461 aggccgcaag gcattgagga gaagctcatg gccggcgcaa aagagatacg tagtaagatc
    23521 gcaagcgtcc agaacacgca aaagatcact aaagcgatgg agatggtcgc cgcttccaaa
    23581 atgcgtaaat cgcaggatcg catggcggcc agccgtcctt atgcagaaac catgcgcaaa
    23641 gtgattggtc accttgcaca cggtaatctg gaatataagc acccttacct ggaagaccgc
    23701 gacgttaaac gcgtgggcta cctggtggtg tcgaccgacc gtggtttgtg cggtggtttg
    23761 aacattaacc tgttcaaaaa actgctggcg gaaatgaaga cctggaccga caaaggcgtt
    23821 caatgcgacc tcgcaatgat cggctcgaaa ggcgtgtcgt tcttcaactc cgtgggcggc
    23881 aatgttgttg cccaggtcac cggcatgggg gataaccctt ccctgtccga actgatcggt
    23941 ccggtaaaag tgatgttgca ggcctacgac gaaggccgtc tggacaagct ttacattgtc
    24001 agcaacaaat ttattaacac catgtctcag gttccgacca tcagccagct gctgccgtta
    24061 ccggcatcag atgatgatga tctgaaacat aaatcctggg attacctgta cgaacccgat
    24121 ccgaaggcgt tgctggatac cctgctgcgt cgttatgtcg aatctcaggt ttatcagggc
    24181 gtggttgaaa acctggccag cgagcaggcc gcccgtatgg tggcgatgaa agccgcgacc
    24241 gacaatggcg gcagcctgat taaagagctg cagttggtat acaacaaagc tcgtcaggcc
    24301 agcattactc aggaactcac cgagatcgtc tcgggggccg ccgcggttta aacaggttat
    24361 ttcgtagagg atttaagatg gctactggaa agattgtcca ggtaatcggc gccgtagttg
    24421 acgtcgaatt ccctcaggat gccgtaccgc gcgtgtacga tgctcttgag gtgcaaaatg
    24481 gtaatgagcg tctggtgctg gaagttcagc agcagctcgg cggcggtatc gtgcgtacca
    24541 tcgcaatggg ttcctccgac ggtctgcgtc gcggtctgga tgtaaaagac ctcgaacacc
    24601 cgatcgaagt cccggtaggt aaagcgactc tgggccgtat catgaacgta ctgggtgaac
    24661 cggtcgacat gaaaggcgag atcggtgaag aagagcgttg ggcgattcac cgcgcagcac
    24721 cttcctacga agagctgtca aactctcagg aactgctgga aaccggtatc aaagttatcg
    24781 acctgatgtg tccgttcgct aagggcggta aagttggtct gttcggtggt gcgggtgtag
    24841 gtaaaaccgt aaacatgatg gagcttattc gtaacatcgc gatcgagcac tccggttact
    24901 ctgtgtttgc gggcgtaggt gaacgtactc gtgagggtaa cgacttctac cacgaaatga
    24961 ccgactccaa cgttatcgac aaagtatccc tggtgtatgg ccagatgaac gagccgccgg
    25021 gaaaccgtct gcgcgttgct ctgaccggtc tgaccatggc tgagaaattc cgtgacgaag
    25081 gtcgtgacgt tctgctgttc gttgacaaca tctatcgtta caccctggcc ggtacggaag
    25141 tatccgcact gctgggccgt atgccttcag cggtaggtta tcagccgacc ctggcggaag
    25201 agatgggcgt tctgcaggaa cgtatcacct ccaccaaaac cggttctatc acctccgtac
    25261 aggcagtata cgtacctgcg gatgacttga ctgacccgtc tccggcaacc acctttgcgc
    25321 accttgacgc aaccgtggta ctgagccgtc agatcgcgtc tctgggtatc tacccggccg
    25381 ttgacccgct ggactccacc agccgtcagc tggacccgct ggtggttggt caggaacact
    25441 acgacaccgc gcgtggcgtt cagtccatcc tgcaacgtta tcaggaactg aaagacatta
    25501 tcgccatcct gggtatggat gaactgtctg aagaagacaa actggtggta gcgcgtgctc
    25561 gtaagatcca gcgcttcctg tcccagccgt tcttcgtggc agaagtattc accggttctc
    25621 cgggtaaata cgtctccctg aaagacacca tccgtggctt taaaggcatc atggaaggcg
    25681 aatacgatca cctgccggag caggcgttct acatggtcgg ttccatcgaa gaagctgtgg
    25741 aaaaagccaa aaaactttaa cgccttaatc ggagggtgat atggcaatga cttaccacct
    25801 ggacgtcgtc agcgcagagc aacaaatgtt ctctggtctg gtcgagaaaa tccaggtaac
    25861 gggtagcgaa ggtgaactgg ggatctaccc tggccacgca ccgctgctca ccgccattaa
    25921 gcctggtatg attcgcatcg tgaaacagca cggtcacgaa gagtttatct atctgtctgg
    25981 cggcattctt gaagtgcagc ctggcaacgt gaccgttctg gccgacaccg caattcgcgg
    26041 ccaggatctc gacgaagcgc gagccatgga agcgaaacgt aaggctgaag agcacattag
    26101 cagctctcac ggcgacgtag attacgctca ggcgtctgcg gaactggcca aagcgatcgc
    26161 gcagctgcgc gttatcgagt tgaccaaaaa agcgatgtaa caccggcttg aaaagcacaa
    26221 aagccagtct ggcaacaggc tggctttttt tacgcgtatt gacccgtcct gaaaagcgtt
    26281 cacatagatc ctgatgatat aaaacacccc tgttttcccg tttattcatt gatcgaaata
    26341 agagcaaaaa catccacctg acgcttaaat gaaggtactg ccttaatttt ctgcagacaa
    26401 aaggcgtgac gatggtcgaa aatggcgctt tcgtcagcgg ggataatccg ttattgaaca
    26461 atttatcctc tgtccatttc acgatgaaaa aaatgtagtt ttttcaaggt gaagcggttt
    26521 aaattcgttc tcaaattaca gtcaggacgc gtatgttgaa taatgctatg agcgtagtga
    26581 tccttgccgc aggcaaaggc acgcgcatgt attccgatct tccgaaagtg ctgcataccc
    26641 ttgccgggaa agcgatggtt cagcatgtca ttgatgctgc gaatgaatta ggcgcagcgc
    26701 acgttcacct tgtgtatggt cacggcggcg atctgcttaa gcaggcgctg aaagacgaca
    26761 acctgaactg ggtgcttcag gcagaacaac tgggtacggg tcatgcgatg cagcaggccg
    26821 cacctttctt tgccgatgat gaagacattt taatgctcta cggcgacgtg ccgctgatct
    26881 ctgtcgaaac actccagcgt ctgcgtgatg ctaaaccgca gggtggcatt ggtctgctga
    26941 cggtaaaact ggatgatccg accggttatg gacgtatcac ccgtgaaaac ggcaaagtta
    27001 ccggcattgt tgagcacaaa gatgccaccg acgagcagcg tcagattcag gagatcaaca
    27061 ccggcattct gattgccaac ggcgcagata tgaaacgctg gctggcgaag ctgaccaaca
    27121 ataatgctca gggcgaatac tacatcaccg acattattgc gctggcgtat caggaaggac
    27181 gtgaaatcgt cgccgttcat ccgcaacgtt taagcgaagt agaaggcgtg aataaccgcc
    27241 tgcaactctc ccgactggag cgcgtttacc agtccgaaca ggctgaaaaa ctgctgttag
    27301 caggcgttat gctgcgcgat ccggcgcgtt ttgatctgcg cggtacgctt actcacgggc
    27361 gcgatgttga aattgatact aacgttatca tcgagggcaa cgtgactctc ggtcatcgcg
    27421 tgaaaatcgg cgccggttgc gtgattaaaa acagtgtgat tggcgatgat tgcgaaatta
    27481 gcccgtatac cgtcgtggaa gatgcgaatc tggcagcggc ctgtactatt ggcccgtttg
    27541 cccgtctgcg tcctggtgct gagttgctgg aaggtgcaca cgtcggtaac ttcgttgaga
    27601 tgaaaaaagc gcgtctgggt aaaggctcga aagctggtca tctgacttac ctgggcgatg
    27661 cggaaattgg cgataacgtt aacatcggcg cgggaaccat tacctgcaac tacgatggtg
    27721 cgaataaatt taagactatt atcggcgacg atgtgtttgt cggttccgac actcaactgg
    27781 tggccccggt aacagtaggc aaaggcgcga ccattgctgc gggtacaacc gtgacgcgta
    27841 atgtcggcga aaatgccctg gctatcagcc gtgtgccgca gactcaaaaa gaaggctggc
    27901 gtcgtccgat aaagaaaaag taattctggc cgggtaatcc ggtcatatgg gatgaggaga
    27961 taacataatc tcccgcccac aagcagtaac tataaaaata accccactct ctacaaggct
    28021 cggggcgccc gaaaaacggg catacaggtt gaccgacaac gatataaatc ggaatcaaaa
    28081 actatgtgtg gaattgttgg cgcgatcgcg caacgtgatg tagcagaaat ccttcttgaa
    28141 ggtttacgtc gtctggaata ccgcggatat gattctgccg gtctggccgt tgttgatgca
    28201 gaaggtcata tgacccgcct gcgtcgcctc ggtaaagtac agatgctggc tcaggcagcg
    28261 gaagaacatc ctctgcatgg cggcaccggt attgctcaca ctcgctgggc gacccatggt
    28321 gaaccttcag aagctaacgc gcatccgcat gtttctgaac acattgtggt ggtgcataac
    28381 ggcatcatcg aaaaccatga accgctgcgt gaagagttaa aagcgcgtgg ctataccttc
    28441 gtttctgaaa ccgacaccga agtgattgca catttggtga actgggagct gaaacaaggc
    28501 ggtactctgc gtgaggcagt tctgcgtgct atcccgcaac tgcgtggtgc gtacggtaca
    28561 gtgatcatgg actcccgtca tccggatacc ctgctggcgg cacgttctgg tagtccgctg
    28621 gtgattggcc tgggtatggg cgaaaacttt atcgcttctg accagctggc gctgttgcca
    28681 gtgactcgtc gttttatctt ccttgaagag ggcgatattg cggaaatcac tcgccgttcg
    28741 gtaaacatct tcgataaaac tggcgcggaa gtaaaacgtc aggatatcga atccaatctg
    28801 caatatgacg cgggcgataa aggtatttac cgccactaca tgcagaaaga gatctacgaa
    28861 cagccgaacg cgatcaaaaa cacccttacc gggcgcatca gccacggtca ggttgattta
    28921 agcgagctgg gaccgaacgc cgatgaactg ctgtcgaagg ttgagcatat tcagatcctc
    28981 gcctgtggta cttcttataa ctccggtatg gtttcccgct actggtttga atcgctggca
    29041 ggtattccgt gcgacgtcga aatcgcctct gaattccgct atcgcaaatc tgccgtgcgt
    29101 cgtaacagcc tgatgatcac cttgtcacag tctggcgaaa ccgcggatac cctggcgggc
    29161 ctgcgtctgt cgaaagagct gggttacctt ggttcgctgg caatctgtaa cgttccgggt
    29221 tcttctctgg tgcgcgaatc cgatctggcg ctgatgacca acgccggtac ggaaattggc
    29281 gtggcctcca ccaaagcatt caccacccag ttaactgtgc tgttgatgct ggtggcgaaa
    29341 ctgtctcgcc tgaaaggtct ggatgcctcc attgaacatg acatcgtgca tggtctgcag
    29401 gcgctgccga gccgtattga gcagatgctg tctcaggaca aacgcattga agcgctggca
    29461 gaagatttct ctgacaaaca tcacgcgctg ttcttgggcc gtggcgatca gtacccaatc
    29521 gcgctggaag gtgcattgaa gctgaaagag atctcttaca ttcacgctga agcctacgct
    29581 gctggcgaac tgaaacacgg tccgctggcg ctgatagatg ccgatatgcc ggttatcgtc
    29641 gttgcaccga acaacgaatt gctggaaaaa ctgaaatcca acattgaaga agttcgcgcg
    29701 cgtggcggtc agttgtatgt cttcgccgat caggatgctg gttttgtggg gagcgataac
    29761 atgcacatca tcgagatgcc gcatgtggaa gaggtgattg caccaatctt ctacaccgtt
    29821 ccgctgcaac tactggctta ccacgttgcg ctgatcaaag gcaccgacgt tgaccagccg
    29881 cgtaacctgg caaaatcggt aacggttgag taataaatgg atgccctgcg taagcggggc
    29941 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
    30001 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
    30061 aatatttgta attaactgaa tataaacgct tatttaaatc agactgaaga cattctctct
    30121 ctgtcataaa actgtcataa accttacata taactgtcac ctgtttgtcc tattttgctt
    30181 ctcgtagcca acaaacaatg ctttatgaat cctcccagga gacattatga aagttatgcg
    30241 taccaccgtc gcaactgttg tcgccgcgac cttatcgatg agcgctttct ctgtgtttgc
    30301 agaagcaagc ctgacaggtg caggtgcaac cttccctgcg ccggtgtatg ccaaatgggc
    30361 tgacacttac cagaaagaaa ccggtaataa agttaactac cagggtatcg gttcttctgg
    30421 tggtgtaaaa cagatcatcg ccaataccgt tgattttggt gcttctgacg cgccgctgtc
    30481 tgacgaaaaa ctggctcagg aaggcctgtt ccagttcccg accgtgattg gcggcgtggt
    30541 gctggcggtt aatattccag gtctgaagtc tggcgaactg gtgctggatg gtaaaaccct
    30601 cggcgacatc tacctgggca aaatcaagaa gtgggatgat gaagccatcg ccaaactgaa
    30661 tccgggtctg aaactgcctt cacagaacat tgctgtagta cgccgcgcag atggctccgg
    30721 gacttccttc gtcttcacca gctacctggc gaaagtgaac gaagagtgga aaaacaacgt
    30781 tggtactggc tctaccgtaa aatggccgat cggtctgggc ggtaaaggta acgacggtat
    30841 cgccgcgttc gttcagcgtc tgccgggtgc agttggttac gttgaatatg cttacgcgaa
    30901 gcagaacaac ctggcgtaca ccaaactgat ctccgctgat ggcaaaccgg tgagtccgac
    30961 cgaagaaaac ttcgctaatg cagcaaaagg cgcagactgg agcaaaacat tcgctcagga
    31021 tctgaccaac cagaaaggcg aagatgcatg gcctattacc tctaccacgt tcattctgat
    31081 ccataaagat cagaagaaac cagaacaagg cactgaagtg ctgaagttct tcgactgggc
    31141 atacaaaacc ggggctaaac aggcgaacga cctggattat gccagcctgc cggatagcgt
    31201 agttgaacag gttcgcgctg cgtggaagac caatattaaa gacagtagcg gcaagccgct
    31261 gtactaataa aactccaggc cgggtacggt gttttacgcc gcatccggca ttacaaaatg
    31321 actttgtaaa cgcgtttaac tgaagagtaa cttatggctg caaccaagcc tgcttttaac
    31381 ccaccgggta aaaagggcga cataattttc agcgtgctgg taaaactggc ggcgctgatt
    31441 gtgctattga tgttgggtgg cattattgtc tctctgatca tctcctcctg gccgagcatt
    31501 cagaaatttg gtctggcttt cctgtggacc aaagagtggg atgcgccgaa cgatatctac
    31561 ggggcgctgg tgccgatcta cggtacgctg gtgacctcgt ttatcgcgct gctgatcgcc
    31621 gtcccggtga gttttggtat cgccctgttc ctgaccgagc ttgcgcctgg ctggctgaaa
    31681 cgcccgctgg gtatcgccat tgagctgctg gctgccattc caagtatcgt ttacggcatg
    31741 tggggcttgt ttatcttcgc cccgctgttc gccgtttact tccaggagcc ggtcggcaat
    31801 atcatgtcga atatcccgat tgtcggcgcg ctgttctccg gcccggcctt tggtatcggt
    31861 atcctcgcgg caggcgtgat cctcgccatc atgattattc cgtacattgc agcggtaatg
    31921 cgcgatgtat tcgaacaaac cccggtgatg atgaaagagt cggcctacgg tattggctgc
    31981 accacctggg aagttatctg gcgtatcgtt cttccgttca ccaaaaatgg ggttatcggc
    32041 ggcattatgt tggggctggg gcgcgcgctc ggtgaaacca tggcggtgac ctttatcatc
    32101 ggtaacacct accaactcga cagcgcctcg ctgtatatgc cgggcaacag tatcacctct
    32161 gcgctggcca acgaatttgc ggaagcggaa tccggtctgc acgtcgctgc actgatggaa
    32221 ctgggcctga tcctgtttgt gattaccttc atcgtcctcg ccgcatcgaa gtttatgatt
    32281 atgcgcctgg ctaagaatga gggggcacgc taatggctat ggttgaaatg caaaccactg
    32341 cggcactggc tgaatcccgc cgcaaaatgc aggcgcgtcg ccgcctcaaa aaccgtattg
    32401 cgctgacgct ctcaatggcg acgatggcct tcggtctgtt ctggctgatc tggattttaa
    32461 tgtccaccat cactcgcggt atcgacggta tgtcgctggc gctgttcacc gaaatgacgc
    32521 caccacccaa tacggaaggc ggtggtctgg cgaacgccct ggcgggtagc ggcctgttaa
    32581 ttttgtgggc taccgtattc ggtacgccgc tgggcattat ggcggggatt tacctggctg
    32641 aatatggtcg taaatcctgg ctggcagaag tgattcgctt cattaacgac attctgctct
    32701 ctgcgccgtc gattgtggtt ggtctgtttg tttacaccat tgtggtggcg cagatggagc
    32761 acttctccgg ctgggcgggc gtgattgcgc tggcgctgtt gcaggtgccg attgttatcc
    32821 gcaccaccga gaacatgctg aaactggtgc cggacagcct gcgtgaagcg gcttatgcgc
    32881 tgggtacgcc gaagtggaag atgatcgcct cgatcacgct gaaagcgtcg gtgtccggga
    32941 tcatgaccgg tatcctgctg gcgattgccc gtattgctgg tgaaaccgca ccgctgctgt
    33001 ttactgcgct ctccaaccag ttctggagca cggacatgat gcagccgatc gccaacctgc
    33061 cggtgacgat ctttaagttt gcgatgagcc cgtttgcgga atggcagcaa ctggcctggg
    33121 ccggggtgtt gatcattacc ctgtgcgtac tgctgctgaa cattctggcg cgcgttgttt
    33181 ttgcgaagaa taaacacggt taacactttg ccggatgcgg cgtgaacgcc ttatccggcc
    33241 tacggtaagc ctgattagcg aagtgcatca ggcacgatga ggaaaagatt gcaatgagta
    33301 tggttgaaac tgccccgagt aaaattcagg ttcgtaattt gaacttctac tacggcaaat
    33361 tccatgccct gaaaaacatc aacctggata tcgctaaaaa ccaggttacg gcgtttatcg
    33421 ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt caacaaaatg tttgaactgt
    33481 acccggaaca gcgtgcggaa ggtgaaattc tgctcgatgg cgacaacatc ctgaccaact
    33541 ctcaggatat cgccctgctg cgtgcgaaag tgggcatggt gttccagaaa ccgacgccgt
    33601 tcccaatgtc catctacgac aacatcgctt ttggcgtgcg tctgtttgag aagctctccc
    33661 gtgctgacat ggacgagcgc gtgcagtggg cattgaccaa agccgcattg tggaacgaaa
    33721 ccaaagataa attgcaccag agcggttact ctctctctgg tggtcagcaa cagcgtttgt
    33781 gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct gctcgacgaa ccgtgttcgg
    33841 cactcgaccc catctctacc gggcgtattg aagagctgat caccgaactg aagcaggatt
    33901 acaccgtggt gatcgtcacc cacaacatgc agcaggctgc gcgttgttcc gaccacacgg
    33961 cgtttatgta cctgggcgaa ttgattgagt tcagcaacac ggacgatctg ttcaccaagc
    34021 cagcgaagaa acaaacagaa gactacatca ccggtcgtta cggttgattc aggagtgcgt
    34081 tatggacagt ctcaatctta ataaacatat ttccggccag ttcaacgccg aactggaaag
    34141 catccgcacg caggtgatga ccatgggcgg catggtggag cagcagcttt ctgatgcaat
    34201 caccgcgatg cataaccagg acagcgatct ggcgaagcgc gtcatcgaag gcgacaagaa
    34261 cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg cgcattatcg ccaaacgtca
    34321 gccgacggcg agcgacctgc gtctggttat ggtgatcagt aaaaccattg ccgagctgga
    34381 gcgtattggc gacgtggcgg acaaaatctg ccgtactgca ctggagaaat tctcccagca
    34441 gcatcagcca ttgctggtaa gtctggagtc gctgggccgt cataccatcc agatgctgca
    34501 cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa gcggtacgta tttatcgtga
    34561 agataaaaaa gtcgatcagg agtacgaagg tattgttcgt caactgatga cctacatgat
    34621 ggaagattcg cgtaccattc cgagcgtact taccgcgtta ttctgtgcgc gttctatcga
    34681 acgtattggc gaccgctgcc agaatatttg tgagtttatc ttctactacg tgaaggggca
    34741 ggatttccgt cacgtcggtg gtgacgagct cgataaactg ctggcagaaa aagatagcga
    34801 taaataattc accaagtaaa tcccaataat ttaat
//
LOCUS       DAZKHK010000043        34512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKHK010000043 DAZKHK010000000
VERSION     DAZKHK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.043"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            147..1007
                     /locus_tag="ACHOS5_002075"
     CDS             147..1007
                     /locus_tag="ACHOS5_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6708 domain-containing protein"
                     /protein_id="HFP9776892.1"
                     /translation="MRTHPHLIKQTIDPRLINEINKRTQSNSEKLSNSDKLTYINDNY
                     CEITRNYTSFYGMNMLSIVLFLPIVILCFASVLFFIYDITFNFERYLDTWRYRADSEL
                     MLHIAYCAIFFMSFVFAAILNYFIFAPRHYPIRFNRKTGKVYICDYILNDMSHKSTYT
                     FWWHYPFYKHTKPEYKEYDWEQIQGIAFFAQNRHSSYSTIRCIVYESLDSTNIIDTFN
                     LITTESAIDALHMTNHKLWLWINSFMNFDDEFLEKSINTAPGIWGREVNWPEDMNKKS
                     MLSSMDEYIL"
     gene            complement(1198..1488)
                     /gene="ilvN"
                     /locus_tag="ACHOS5_002076"
     CDS             complement(1198..1488)
                     /gene="ilvN"
                     /locus_tag="ACHOS5_002076"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9776893.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1492..3180)
                     /gene="ilvB"
                     /locus_tag="ACHOS5_002077"
     CDS             complement(1492..3180)
                     /gene="ilvB"
                     /locus_tag="ACHOS5_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP9776894.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(3286..3384)
                     /gene="ivbL"
                     /locus_tag="ACHOS5_002078"
     CDS             complement(3286..3384)
                     /gene="ivbL"
                     /locus_tag="ACHOS5_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP9776895.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            complement(3629..3703)
                     /gene="ysdE"
                     /locus_tag="ACHOS5_002079"
     CDS             complement(3629..3703)
                     /gene="ysdE"
                     /locus_tag="ACHOS5_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP9776896.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            3785..4969
                     /gene="emrD"
                     /locus_tag="ACHOS5_002080"
     CDS             3785..4969
                     /gene="emrD"
                     /locus_tag="ACHOS5_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP9776897.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4977..5474)
                     /gene="yidF"
                     /locus_tag="ACHOS5_002081"
     CDS             complement(4977..5474)
                     /gene="yidF"
                     /locus_tag="ACHOS5_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9776898.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5471..5833)
                     /gene="yidG"
                     /locus_tag="ACHOS5_002082"
     CDS             complement(5471..5833)
                     /gene="yidG"
                     /locus_tag="ACHOS5_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP9776899.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAVKHNWH
                     QAGMLFWISIGILAIVALILWHNTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(5823..6170)
                     /gene="yidH"
                     /locus_tag="ACHOS5_002083"
     CDS             complement(5823..6170)
                     /gene="yidH"
                     /locus_tag="ACHOS5_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP9776900.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(6230..7723)
                     /gene="yidJ"
                     /locus_tag="ACHOS5_002084"
     CDS             complement(6230..7723)
                     /gene="yidJ"
                     /locus_tag="ACHOS5_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9776901.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDQNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(7720..9435)
                     /gene="yidK"
                     /locus_tag="ACHOS5_002085"
     CDS             complement(7720..9435)
                     /gene="yidK"
                     /locus_tag="ACHOS5_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP9776902.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPIFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAVVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     MGILFAMIGVYAGLAEFGGYGTRWLAIISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            9602..10468
                     /gene="yidL"
                     /locus_tag="ACHOS5_002086"
     CDS             9602..10468
                     /gene="yidL"
                     /locus_tag="ACHOS5_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9776903.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHTVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(10558..12219)
                     /gene="yidE"
                     /locus_tag="ACHOS5_002087"
     CDS             complement(10558..12219)
                     /gene="yidE"
                     /locus_tag="ACHOS5_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP9776904.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(12416..12844)
                     /gene="ibpB"
                     /locus_tag="ACHOS5_002088"
     CDS             complement(12416..12844)
                     /gene="ibpB"
                     /locus_tag="ACHOS5_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP9776905.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(12956..13369)
                     /gene="ibpA"
                     /locus_tag="ACHOS5_002089"
     CDS             complement(12956..13369)
                     /gene="ibpA"
                     /locus_tag="ACHOS5_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP9776906.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(13363..13435)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            13675..14007
                     /gene="yidQ"
                     /locus_tag="ACHOS5_002090"
     CDS             13675..14007
                     /gene="yidQ"
                     /locus_tag="ACHOS5_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9776907.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEADAQETNNVI
                     PPAKMPAN"
     gene            complement(14011..15225)
                     /gene="yidR"
                     /locus_tag="ACHOS5_002091"
     CDS             complement(14011..15225)
                     /gene="yidR"
                     /locus_tag="ACHOS5_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP9776908.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHTKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSAKGEKVPELFIVELPQDEAGWKVAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHGNPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            15326..16426
                     /gene="cbrA"
                     /locus_tag="ACHOS5_002092"
     CDS             15326..16426
                     /gene="cbrA"
                     /locus_tag="ACHOS5_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP9776909.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYRRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(16387..17724)
                     /gene="dgoT"
                     /locus_tag="ACHOS5_002093"
     CDS             complement(16387..17724)
                     /gene="dgoT"
                     /locus_tag="ACHOS5_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776910.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17799..18947)
                     /gene="dgoD"
                     /locus_tag="ACHOS5_002094"
     CDS             complement(17799..18947)
                     /gene="dgoD"
                     /locus_tag="ACHOS5_002094"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP9776911.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(18944..19561)
                     /gene="dgoA"
                     /locus_tag="ACHOS5_002095"
     CDS             complement(18944..19561)
                     /gene="dgoA"
                     /locus_tag="ACHOS5_002095"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP9776912.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(19545..20423)
                     /gene="dgoK"
                     /locus_tag="ACHOS5_002096"
     CDS             complement(19545..20423)
                     /gene="dgoK"
                     /locus_tag="ACHOS5_002096"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP9776913.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENAADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(20420..21109)
                     /gene="dgoR"
                     /locus_tag="ACHOS5_002097"
     CDS             complement(20420..21109)
                     /gene="dgoR"
                     /locus_tag="ACHOS5_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP9776914.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            21388..22044
                     /locus_tag="ACHOS5_002098"
     CDS             21388..22044
                     /locus_tag="ACHOS5_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776915.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(22088..22900)
                     /gene="yidA"
                     /locus_tag="ACHOS5_002099"
     CDS             complement(22088..22900)
                     /gene="yidA"
                     /locus_tag="ACHOS5_002099"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9776916.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRITLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(23015..23413)
                     /gene="yidB"
                     /locus_tag="ACHOS5_002100"
     CDS             complement(23015..23413)
                     /gene="yidB"
                     /locus_tag="ACHOS5_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP9776917.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQAHNDLLSAGMELLKGKLFR"
     gene            23531..23632
                     /locus_tag="ACHOS5_002101"
                     /pseudo
     CDS             23531..23632
                     /locus_tag="ACHOS5_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23653..26067)
                     /gene="gyrB"
                     /locus_tag="ACHOS5_002102"
     CDS             complement(23653..26067)
                     /gene="gyrB"
                     /locus_tag="ACHOS5_002102"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9776918.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(26096..27169)
                     /gene="recF"
                     /locus_tag="ACHOS5_002103"
     CDS             complement(26096..27169)
                     /gene="recF"
                     /locus_tag="ACHOS5_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9776919.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(27169..28269)
                     /gene="dnaN"
                     /locus_tag="ACHOS5_002104"
     CDS             complement(27169..28269)
                     /gene="dnaN"
                     /locus_tag="ACHOS5_002104"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9776920.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(28274..29677)
                     /gene="dnaA"
                     /locus_tag="ACHOS5_002105"
     CDS             complement(28274..29677)
                     /gene="dnaA"
                     /locus_tag="ACHOS5_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9776921.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            29974..30054
                     /locus_tag="ACHOS5_002106"
     CDS             29974..30054
                     /locus_tag="ACHOS5_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9776922.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            30284..30424
                     /gene="rpmH"
                     /locus_tag="ACHOS5_002107"
     CDS             30284..30424
                     /gene="rpmH"
                     /locus_tag="ACHOS5_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9776923.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            30441..30800
                     /gene="rnpA"
                     /locus_tag="ACHOS5_002108"
     CDS             30441..30800
                     /gene="rnpA"
                     /locus_tag="ACHOS5_002108"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9776924.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            30764..31021
                     /gene="yidD"
                     /locus_tag="ACHOS5_002109"
     CDS             30764..31021
                     /gene="yidD"
                     /locus_tag="ACHOS5_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9776925.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            31024..32670
                     /gene="yidC"
                     /locus_tag="ACHOS5_002110"
     CDS             31024..32670
                     /gene="yidC"
                     /locus_tag="ACHOS5_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9776926.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            32776..34140
                     /gene="mnmE"
                     /locus_tag="ACHOS5_002111"
     CDS             32776..34140
                     /gene="mnmE"
                     /locus_tag="ACHOS5_002111"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9776927.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNNVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            34382..34456
                     /gene="tnaC"
                     /locus_tag="ACHOS5_002112"
     CDS             34382..34456
                     /gene="tnaC"
                     /locus_tag="ACHOS5_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP9776928.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
BASE COUNT         8391 a         9206 c         8666 g         8249 t
ORIGIN      
        1 actctctaaa taattcgcag acacagcaca tgcaacttga agtatgacaa gcatgcctgc
       61 ttcattatta cactttaata ataaaaccca ccgattaatg tacaaaaaaa tccctgcgtg
      121 atattgtctg taaaaaagga gcagatatgc gcactcaccc tcacttaata aaacaaacta
      181 ttgatccacg actcattaat gaaataaaca aaagaacaca aagcaacagt gagaagttat
      241 caaacagtga taaattaaca tatattaatg acaactactg tgagataacg cgaaactaca
      301 cgtcatttta tggtatgaac atgctgagca tcgttctttt cttacctatc gttattttat
      361 gttttgcctc tgttttattc tttatatatg acataacatt taacttcgaa cgatacttag
      421 atacgtggag atacagagca gactctgagt taatgctaca tattgcttac tgcgcaattt
      481 ttttcatgtc ttttgttttt gctgctattc taaattattt tatctttgct ccgcgacatt
      541 acccaatccg ttttaataga aaaacaggaa aggtctatat ttgtgattat attcttaatg
      601 atatgagtca taaatcaacc tatactttct ggtggcacta tccattctat aaacatacca
      661 agcctgaata taaagagtac gactgggaac aaattcaagg tatagctttt tttgcacaaa
      721 atcgccattc ctcatactcc actattcgct gtattgttta tgaatcatta gacagtacaa
      781 atattattga cacctttaac ctgataacga cagagagtgc tatagatgca ttgcatatga
      841 caaatcacaa attatggtta tggattaata gctttatgaa ttttgacgat gagtttttag
      901 agaaatctat caatactgct ccaggtattt ggggccgtga agtgaattgg cctgaagata
      961 tgaataagaa atcaatgttg tcttcaatgg acgaatatat actctgaata attcgagtta
     1021 cagaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc tccccccagt
     1081 cacttacgta tgtaagctcc tggggattca ctcgcttgtc gccttcctgc aactcgaatt
     1141 atttagagta tataagctcg cccggatgta aatatcccat ccaggcgaga aaatcactta
     1201 ctgaaaaaac accgcaatct tgttaaacat cgtcggatcg gactgattac gctgcacttt
     1261 caccacatct tccagcttgt ctatctggct tatcatctgc tccagacgct ggtcgtcatt
     1321 gaccagtagc cagatgtggc ttttgtcgct gtcctgaatc ggcaggcaga gaatgccttc
     1381 aacgttaaaa gcgcgacggg caaaaaggcc acaaacgtgg gtcattacac ccggatggtt
     1441 gcgaacggtg agttccagaa tcacgttatc atgagttgtg ttttgcatgg cttattcccc
     1501 caccatttca gtattcgccg cacctggcgg caccatcggg tacacttttt cttcggcatc
     1561 aatgcgcaca tggatcagcg ccgggccagg gcgattgatg atttcctgca atgcagcctg
     1621 cggatcggct tcgttattca aatcacaggt ttcgaggccg aatcctgcgg caatctgcat
     1681 aaagttgatt ttgcccggat aggtagcggc aaaaacgccc tgcttgtaga acagactctg
     1741 ttgctgatgc accagcccca gcgcttcgtt gttcatcaaa atgattttaa catccagctg
     1801 attttcactg gcggtcgcca tctcctgaat attcatcatc aggctgccgt cgccggagaa
     1861 gcacaacact ttgcggtccg ggttcgccag cgccgcgcca atcgccgcag gcaggccaaa
     1921 acccatcgtg cccagcccgc cggaggtcag ccactggcgt gggcgattga gcggataggc
     1981 ttgcgccgtc cacatctgat gctgaccaac atcagtggta ataatcgcgt tgtcatccac
     2041 gcaggcggca acggcattaa tcaggccgta atggcttaat ggatcgcacg ctttcgggat
     2101 tggacacggg aattcacgct gcaaatccgc caccaactgg tgccactctg cacgcggttg
     2161 cgcttccacc agcgggatca actgcgccag cacgtcatca acatccgcct gaattgccac
     2221 atgcggttgc ttgattttgc ccagctctgc gcggtcgata tcgacatgaa taattttggc
     2281 attcggacag aactgctcgg ttttgccaat cgcccggtca tcaaaacgcg caccgagtac
     2341 aatcagcaga tccgcctcct gcaaaatata gttggtgctg cgcacaccgt gcatccccag
     2401 catacccagc gacagcggat gcgcttttgg caacataccc agcgccatta aagtcatggt
     2461 ggtaggcagt tgcgctttct ccgccagttc acgcactcgc gcaggcgcgt tgatcacacc
     2521 accacccaga taaagcaccg ggcgtttggc ggcgttaatc atcgccgccg cgtcacgaat
     2581 actttcttcg ctaaaggcgg gtgcggcggc tttttctgcc atagcgggct gtgtttcaat
     2641 ctcaaaaact gccgtttgca catccttagg aatgtctatc cacaccgggc cagggcggcc
     2701 tgattgcgca atgcggaagg catcgctcat gacctgcggg agttcttcga tatgtctgac
     2761 cagatagttg tgtttggtga tggggataga gatgccgtag gtgtcgactt cctggaaggc
     2821 gtcggtgccg atcatcgagg cgggaacctg accagtgatg caaatcagcg ggatggagtc
     2881 cagcctcgca tcggcaatgg cggtcaccag gttagtcgca cccggtccgc tacaggccat
     2941 acagaccgcc ggtttaccgt cggtgcgcgc cattccctga gcgataaacc ccgcgccctg
     3001 ttcatggcga gccagaatat ggcggatttg cgtgctttgg cttaaggcat cgtaaacagg
     3061 caggatagaa ccgcccggaa tacccgtcac aatcttaatg ccctgctgtt ccaggaaatg
     3121 aacgataaat tctgcgccgg taaagcgctt acgcgtcgat gttgtgcccg aacttgccat
     3181 gctccagtcc ttttcttctg ggccgacttt ccgggaggag cttaaacgaa aaaccccgcc
     3241 cggtttgcgc cggcggggtt ttggaatcgt gtgttgttcc agtccctacg gcgcattgcc
     3301 gacgaccacc accacacgca cgacgaccac tgcggcggat ggcgcagttg gtagtagttt
     3361 tgcgttgaac atggaagtag tcattgggga ccttgttggt tttgtgttta acaatattta
     3421 tacaagcaca gctttacagg ggagacaatg gaaaattttt cttccggata aaaatacgtg
     3481 gcagatcaca ttttgaactg aaactcaggc aataaagcat catcacctga aagacggtca
     3541 gattgttgac agagtgcgcc ttattcatgt cgaatacagc gtaaacacct taccctacca
     3601 accgcaccat cactgtttcc ggcaacattt attgccgctt ttgtttacat attctgccgc
     3661 taaacaattc cccattcctg gcgtatatct ggctaacatt catcaatgtg atagattcct
     3721 ctcccgcatt tatgggaatg cgtagtgact tattctaatt atttttataa aagcatccgt
     3781 gataatgaaa aggcatagaa acgtcaattt gttattgatg ttggtattac tcgtggccgt
     3841 cggtcagatg gcgcaaacca tttatattcc agctattgcc gatatggcgc gcgatctcaa
     3901 tgtccgtgaa ggggcggtgc agagcgtaat gggcgcttat ctgctgactt acggtgtctc
     3961 acagttgttt tatggcccaa tttccgatcg cgtgggtcgc cgaccggtga tcctcgtcgg
     4021 aatgtccatt tttatgctgg caacgctggt cgcggtcacg acctccagtt tgatggtatt
     4081 gattgccgcc agcgcgatgc aggggatggg caccggcgtt ggcggcgtaa tggcgcgtac
     4141 tttgccgcgc gatttatatg aacggacaca gttgcgccac gctaacagcc tgttaaacat
     4201 ggggattctt gtcagtccac tgctcgcacc actaatcggc ggtctgctgg atacgatgtg
     4261 gaactggcgc gcctgttatc tctttttgtt ggtactttgt gccggtgtga ctttcagtat
     4321 ggcccgctgg atgccggaaa cgcgtccggt cgacgcaccg cgcacgcgcc tgcttaccag
     4381 ttataaaacg cttttcggta acagcggttt taactgttat ttgctgatgc tgattggcgg
     4441 tctggccggg attgccgcct ttgaagcctg ctccggcgtg ctgatgggcg cggtgttagg
     4501 gctgagcagt atgacggtca gtattttgtt tattctgccg attccggcgg cattttttgg
     4561 cgcatggttt gccggacgtc ccaataagcg cttctcaacg ttgatgtggc agtcggttat
     4621 ctgttgcctg ctggctggct tgctgatgtg gatccccgac tggtttggcg tgatgaatgt
     4681 ctggacgctg ctcattcccg ccgcgctgtt ctttttcggt gccgggatgc tgtttccact
     4741 ggctaccagc ggcgcgatgg agccgttccc cttcctggcg ggcaccgctg gcgcgctggt
     4801 cggcggtctg caaaacattg gttccggcgt gctggcgtcg ctctctgcga tgttgccgca
     4861 aaccggtcag ggcagcctag gattgttgat gaccttaatg ggattgttga tcgtgctgtg
     4921 ctggctaccg ctggcgacgc ggatgtcgca tcaggggcag cctgtttaag ctcacgtcac
     4981 cgcagcatcg tcatcagctc catgggagaa cgatgctgct ttatcagatc acgcatcacc
     5041 cgcatatgcg gcgcggagta agaataaaac gcctgatagc ccgcacaaag cacgttgtct
     5101 ggtttatctt cccgagcgtg cgggcacagc cgatgccagt cgcacgcctg gcagtcacgg
     5161 cgcggttgcg acttttcgct gccacaccat ttttgtaagg tggtgtcaaa aagttcaatc
     5221 gaaatacgac tgatatctgc ccgcacccaa cactcgaaca gggcaattaa aaatttcccc
     5281 cacagctctg ctgtcaccga agcggccgtc agatcgcccc ggtcatcaaa ttcaagatgg
     5341 ggaataagct gtactgagcg gatgcccagc tgtttgaaat tgtgataaaa atcggcaggt
     5401 tgcagagcat ctttatactc tacaaccagt ttatgcctgt cctcttcagc atcaatgacc
     5461 tgacttcctg tcatgcgact ctctcaataa atacgataag ttgatgtata tgcgttacag
     5521 caaacagtat tgcgagagat aacaccgcga gggagatcaa aaatttgtca cggactacgt
     5581 gaaattggga aaaatcgcta ttcgtgacat ccattaaatt gcgattacgg gtgttgtgcc
     5641 agaggatcag cgccacgatg gcgaggatgc caatcgaaat ccagaataac atgcccgcct
     5701 ggtgccagtt gtgtttgaca gccaacgcca tcaacgcgcc gtagcccagc atagtacgaa
     5761 accacgccag tgatgtgcgt tccggctgca gccctgggtc ggcaatgcgt ctggctttgc
     5821 ggctatccgg catacaacac cagtcccatg acgatcactg ccacgaccat caatattaaa
     5881 ctgatgatta ataagctgtt ggtatagggc aggtcctctt tcaggcgcat cgctttttca
     5941 ttgcgtaacc agcgcaagta gccatacatc gctaacccgc cggaaaacag acacaacaac
     6001 aacgccagca gttcgcggat gacgggcgtg gcgaaatctg gcgcaagttg atcaagccca
     6061 acccccgccg ccagaaatcc tagtgcagta cggatccacg ccagaaaagt acgctcatta
     6121 gccagcgaga agcggtaatc gggcgcttct ccgaggcggg aaattttcat tgttgctcct
     6181 tgtaaactgt tcctcgccga aaaattaggc caggaacagc aataatgcat cagaacttct
     6241 gttttttctc ttccaccttc accccttgtg tcggtaaacc ggtgtcatag tcgcgtacca
     6301 ctggagaata accatcctgt gggcgtggac gaaacgcccc catccagcgc ggttgtgcat
     6361 ctttgcgcca cggacgcagg ttccattgat aactgcggaa cggatcgcga attttgtcca
     6421 tgtaatccaa taaggcgtca tgcattttgc tgcgaacgtc ggcaaaatgg atatcatcga
     6481 tcaggttatg catttcattt tggtcattac ggcgatcgta aagttcatca ctggtgaaga
     6541 ggttgagcac cagtttaaag tcatccgtta cccagcaacg caccggaata aaaccgccaa
     6601 agctgtcatg ctcaatctcg tagcgattaa attccaccaa cacgccacgt ggttctttta
     6661 cggcaaggat attttccccc ggcagaatct ctggtttttc aatatctgcc agcgccatca
     6721 ttgtcggcag taagtcaata tgactgactg gcgtatcgac ctgtcgccgc tccccttgcg
     6781 gcgaacggat gatcagcgga atgcgggtga tgtcgtcata catcgccgct cctttactga
     6841 tcagcttatg tgcgcccatc atttcgccgt gatcggaggt ataaataacc cacgtatttt
     6901 cacgttgctc tggcgttaag gcattgatga cccgtccgat ttggtcatca acaaagtcat
     6961 tgcaggcaaa atagagcgga tggtgataaa gcccgtcatc accgactggc gatggcatcg
     7021 cctgcgccca taagcggtga tgttccggtt tgttcgccag gtcatcctct gctttctcac
     7081 ccagttcgta gtaaaaatca gtgtatttct ctaaatactc caccggacag gtgaacgggt
     7141 gatgcggctc atcatacgaa atcaccatca ggaaaggttc gtcggcgcgc gcgggctgtt
     7201 gcagaaaatc caccgcccga ttgctgatgc ggtgcgccca ggtgaaggtt tcgtcaatat
     7261 ggttcgcctg taaatcctca acgctgttta ggccattgcg ccacaggcta atctctttct
     7321 ccgtcagttc actaagatag ttcgcgccat cgaaccagta atcagcgtcc cactccggcg
     7381 gacactcgcc agtgccgaaa tagtcatgtc catcgagatg ccatttgccg atgtagcagg
     7441 tgtgatagcc cgcatcctta aagtagcgcc ccatggtgga aatgtttttg cccggcgcga
     7501 cgttgttggt ccacgggccg gactggttag cgtagatacc ggtaaacagc ccggcgcgcg
     7561 ccggtgtaca aaccggtgaa caggtgtagg cggaattaaa gcgaatacct tctgccgcca
     7621 gactatcaat attttgcgta ttcagcggtt taccgctata gcaaccgacc atattggtgg
     7681 cctgggtatc ggtcatgatg aacagaaaat tggggcgttt cataggctat ccttcgcatc
     7741 gggagtaaag gtgacggctg ggtcgtgacg atgccgccag ctgtcaaaaa tcaggtagac
     7801 aatcactacg gcggcgatga aataactgat tatcgctaac cagcgcgtac cgtagccacc
     7861 gaattcagcc agcccagcat agacgccaat catcgcgaac aggataccca ttgacgcaat
     7921 cttgacgttt ttccacggtt tcatgtccac cgcaaacgca tctttgaagg tgaacggcgt
     7981 ggcgcgcggt ttgataaaac cgatcaccag catcacgacc acgttgatgc agaacgtaca
     8041 ggccagcaca tagaggaaat ggaagtcgaa cttcaccaga tagttgatgg tgatgtagct
     8101 gattatgcca atccccatcg ccacttttgc cgccagcgcc ggaatgcgcg ggaagaaaaa
     8161 gcccataatg atgatggtaa ccagcggcac gttgtaaata ccgttgagct gtttcatcca
     8221 gctatacagc ccctgcggcg cgttggcgat ccacggcgcg accatcaccg aaacgacggc
     8281 gataaagaaa ccaaatttgc gcccgacggt gaccagctgt tgcggctcgg cattctggtt
     8341 aatgatgcgc cggtaaatac ccatactgaa taacgtactg gcgctattca gaaagccgtt
     8401 gaaggtactg atcaccgcac caaataacac cgcaccgaag aagcccacca gtggcaccgg
     8461 cagaacgtta ttgaccagcg tcgggtaggc catgtcggct ttcggtaaat cctgatacag
     8521 atgaaacgca atcaaccctg gcagcaccag taccagcggg tcgagcattt tcagcaccgc
     8581 cgttaacagc gccccctttt gcccttccgc caggcttttt gacgccagcg tgcgttgcac
     8641 gatgccctga tttgtacacc agtaaaaggt gttcaccaga atcaaaccgg taaatgccgc
     8701 gccaatcggc aagggatcgg tcgggccgcc gactgagttt aatttctcgg cgtgaacggt
     8761 ggtgagttgc tcaatgccct gcataaagct gcccttgccc atcgcgatca ggccaaatat
     8821 cggcaccatc aacccgccaa taaccagccc aataccgtta atggagtccg ccactgccat
     8881 tgcgcgcagt ccgccgatca ccgcatacaa aattcccgcc aaaccaagca aaatcaccag
     8941 taaccagata gccgcaccat cggaaagttg tagcgactca cccacatgaa acaggctgtt
     9001 gagcgccaac gcgccggagt agagaacaat cggcagaaag cagacgccgg tggcaatgag
     9061 gaagcagaag tcgatgataa tacgcgtcgt tttatcataa cgttcctcca gaaaatcggg
     9121 gatggtggca atgccgcgct tcagatagcg cggtaaaaag attagcgcga ggaagatcag
     9181 cgtcactgca gaggtgactt cccagcccat caccgacatg ccgcttttgt aggcctgccc
     9241 ggaaaggccg accagttgtt ccgtggaaag gttggttagc attaacgaag cggcaataac
     9301 cggcgctttt agtgaacgtc cggcaagaaa atagccttgt tgcgatccag tatctgtttt
     9361 gcggactttc caccaggtga tgaccgccac cagcagcgta aaaccgacaa aactcaagat
     9421 ttgtaacgaa ttcatctcat agcccttatt tatgtttatg tcccggacgc atgaatatag
     9481 ggagaggtaa aatgcgtcgg gtcaggcagt cagattctta aaatacaaac gtcatatccc
     9541 tgaacggatt gttttctgtt aagttcaggt tgtgagcata attcgccgga gggatattaa
     9601 aatgaatgga aaattgcaaa gttcggatgt aaaaaacgaa actccgtaca atattccctt
     9661 attgattaac gaaaatgtga tctccagcgg aatttctctg atatcgctct ggcataccta
     9721 cgccgacgag cattatcggg tgatctggcc gcgggacaag aagaaaccgc ttatcgccaa
     9781 ctcatgggtt gcggtgtata ccgtacaagg atgtgggaaa attcttttaa agaatgacga
     9841 acaaataact ctgcatggca actgtattat atttttaaag ccaatggata ttcactctta
     9901 tcactgtgaa ggtttggtct gggaacagta ctggatggaa tttaccccca ccagtatgat
     9961 ggatattccc gtcggtcagc aaagcgttat ttataatggc gaagtttata atcaggaact
    10021 caccgaagtt gctgagttaa ttacttcacc agaagcaata aaaaataatc tggcagtcgc
    10081 ttttctgacg aaaattattt atcagtggat ttgtcttatg cacacagtcg gtaaaaaaga
    10141 tccacaacgg cggcaaattg aaaaattaat tgccacttta catgccagtc tacaacaacg
    10201 ctggagcgta gctgatatgg ctgccacgat cccctgtagc gaggcctggt tgcgtcgtct
    10261 gtttttacgc tataccggca agacgccgaa agaatattac ctcgatgcgc gtctggatct
    10321 ggcgctatcg ctattaaagc aacaagggaa ctcggttggc gaagtcgctg atacgctcaa
    10381 cttcttcgac tcctttcatt tcagcaaagc ctttaagcat aaatttggtt atgcgccgtc
    10441 agctgtgctg aagaatacgg acaggtaata accagaatgg cgagtgtgta aatgactcga
    10501 tgatgttcag aaataccagg cggagtacca tttacggcga ctccaccggg aaaagagtta
    10561 accgatactc cagaagagca ccgccagtaa ttggggtgtg ataatgcgca ggaacatcac
    10621 taacggatag acagtggcgt aagagagcgc cgccgcaccg ctggttggat gaagattatt
    10681 agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc acatggtcag
    10741 gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg gaacggcggt
    10801 gatcagggca ccataaccaa tccagcttag cccttcgcca tccaccagtg tatggataaa
    10861 atcgccaccg gatttcaggc caaccaccga gagaaagagc acaatgccta actcgcgtag
    10921 cgccaggtta gcgcttggtg gcataaacca gtacagcttg ccgatactgc cgatacgccc
    10981 gaggatcaac gccataatca gcggaccgcc cgccagcccg agtttcaacg ccgccgggaa
    11041 cccaggcaca aagacgggaa tagaacctaa caatacgcca agcccgatac caataaacac
    11101 tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat tggcaacggc
    11161 atcaattgcg gacggacgcc ccaccaggtt gaggatatcg ccgaactgca ggctgatatc
    11221 gctacttgca actaattcaa caccagcacg gttcaggcgc gagataacaa cgtcatagcg
    11281 ttctttaaag tgcaggtcgc gaatacgttt accgagcacg ttttcattgg tgaccaccac
    11341 acgcgcgacg cgcaaatcag tgcctttcgt agacagcgag gtatcgacct cctgaccaat
    11401 caccagttgc gcattatgta aatccgcagg ctgccccacc agatgcagca aatcgcccag
    11461 ttgaataacg gtttctggcg aaggcacttt tagggtttct tctcgtttca atcgtgagca
    11521 aataacttta tcgccattga gaatcggcac atctttaatg gctaaattat gcaaattagg
    11581 attctcaact cggatgttaa tcgtcctgat tagcgcaccg ccgttggttc gcgtggactc
    11641 gtgctgctgg gcctcagtct caacattgac gcggaaaata acccgcagca tccacatagt
    11701 gaacaaaatg ccgcaaatcc caaatggata cgccatcgca tagctcatcc ccatttgatc
    11761 gaccatcgcc atcggtgtcc ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg
    11821 cgtattggtg accgcgccgg aaaaaatccc cagcactacc ggcagtggaa tatcaaacag
    11881 tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag caaacaggtt
    11941 gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctacct gaatcccgat
    12001 ggtataaacg aacaggatca ggccaaactc ctgaataaca tgcagcatat cgctacttaa
    12061 tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccgaaca gcacgccgcc
    12121 aatgcccaat ccaacaccgc gaaacttgac gttgccgata aacaacccga cgaccgccac
    12181 caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct cttgaaataa
    12241 cattttaatt gctacggatt atggctgaat cggtgaagca gatttgcctt atagcgcaca
    12301 ttatcacggg aaagaacgtg ccgaaatatc ttaaacaggc gaaatttaac gcattgctga
    12361 agaaaaaaga caaaaaaaag ccccaccaga atggcggggc aaagagaata gctagttagc
    12421 tatttaacgc gggacgttcg ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat
    12481 tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt tgcgccagag acttccatat
    12541 tttcagccag cgtaaagctc agactaaaag gctgattcat aagcccttga tgcagccatt
    12601 ttttctcttc ttttggctgc tccggcgtgc cttttacgct caggcgcgta ccttccagtt
    12661 gaatctctaa atcttcctga cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat
    12721 cgtcgctttt ctcaatgttg tacggcggga agctctggct ttcaccggcg ttttgcagtg
    12781 cgttggccag tttgtcaaaa ccgatccatt gacgcatcag tggggataaa tcgaagttac
    12841 gcatagtcat ttctccttct aagaagcgag taagtacctg caaatccgaa gattcgcata
    12901 tgctccctga cggcgagcat ggaaatgtca ggccgcgcca ggcggcctta gggaattagt
    12961 tgatttcgat acggcgcggt tttttcgctt ccggaatcac gcgttcgaga tcgatataca
    13021 gcagaccatt caccaggtta gcaccacgaa catgaatgtt ctcagctaac tggaatttac
    13081 gttcaaagtt gcgttcagcg atgccctggt acagataggt gcgctctttt tgttcgtcgg
    13141 cgtgagcacc tttcaccacc agcagattat cctgggcggt aatttccagt tcgctctcag
    13201 caaaaccagc cacagcgata gcaatgcgat aatggttttc gtctaccagt tcaacgttat
    13261 acggagggta gccgccatta ctctggctct ggttgttttc taagtggtta aacaaacggt
    13321 caaatccaat agcagaacgg taaagcgggg ataaatcaaa gttacgcata atcaatagct
    13381 cctgaaatca gcgagaatct aagaccttcc acaatggaca ggtcaggtag ccagaacacc
    13441 catcaggcgc gtcctcatcg gctacgatgt aaaaatgggt ctggaaatga acttttcaag
    13501 ccttatcagc ggactttttt ttgcagttta tggtctattg catagactga gggggcagca
    13561 cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa tgatgatgtt attttcagca
    13621 agggatcgct ataactcatc aggccaacac ccacgatcaa cagatgaatt tattatgata
    13681 agaaatgtgt tgttagcgtt catgatatgc agcggaatga cattactcgg aggatgctcc
    13741 agcgtgatgt cccataccgg ggggaaagaa ggaacgtatc ctggcacccg cgccagcgcc
    13801 accatgattg gcgacgatga aaccaactgg ggcacaaaat cgctggcgat actcgacatg
    13861 ccatttaccg cagtactgga cacaatattg ctaccgtggg atgtgttccg caaagacagc
    13921 tcagtgcgct cgcgcgttga aaaaagcgaa gccgacgcgc aagaaaccaa taacgtcatt
    13981 ccccccgcca aaatgcctgc aaactgagag ctagcggtca gtttcggtga tccacaactg
    14041 accgtcctcc atccacgcca accattgacc atccggcgag aaaaccacgg cgtccgcaga
    14101 aggtggattg ccgtggtttt cggttaaata ctcaacctcg ccgctttgcg catgggcaca
    14161 agcaatccga ttatccagca caaatcccag ccattcgcct gatggatgcc agttaaatgc
    14221 agactgaata tccgttttat tatgggttaa ctggcgtggt tcaccgccct gtggcgagat
    14281 aagccacagt tgcacaatgc cgttatcatc acgcattaaa aacgcgattt gcgtaccctg
    14341 cggattacag cgcacccagt ggcgcgggac gttgactaat cccggataag cccgatggtg
    14401 cgtaaaggtt aaacgtcgct gcacgacacc acgcggtggc gcaggcaggg ttgtttccgt
    14461 tccgcttaac ggcgcatcac ctgccacttt ccagccagct tcatcttgcg gcaactcgac
    14521 aataaacagc tccggcactt tctcgccctt tgccgaaagt gtgtcgccaa taaacgccag
    14581 cgcgtgattt cctacccatc cttcttcata agcacgattg atttcatcac tgccaggctg
    14641 tggcgtgggc gtggttttac ttaccagcac gcaccagtgg ctaccgctgt attcacgcgg
    14701 atgctgcttt tgtacgttga ccgggccaaa cggtgcagca acaccgacat ttcgcaaatc
    14761 cagcgccgga tcgagttcat gcattacatg gtcgttgtag gtaaagctca ccctttcacc
    14821 gttcgggcta aagacatgta catgactgcc gccgcgcagc gctcctggag tatatggcgc
    14881 ggtaatatcc attgcatcga gattgctcac cttgccgctt tcaacaatca ctccgcgccg
    14941 atgatgaaag tcgtaatacc atgtttcatc aggattttcc gggccgtgga tgaagacata
    15001 cttctctgac ttcgtatgaa cggtcaccac gccgacgtat gcgccctgtg acgcgcgata
    15061 gataacctcg acctcgccgg tatgaatatt cacacgctca atggtctcgc cggtaaacga
    15121 cgccccagaa ggccgcacgt caaacaccag ccactggctg tcgggcgtcc aggtattggt
    15181 attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgt ggtcctgata
    15241 caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat agtcttttgc
    15301 catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta ttggcctcgg
    15361 cccggcaggg tcggcgttgg cgcgaaagtt agcaggcaaa atgcaggtga tcgcgctgga
    15421 taaaaagcac cagcatggta ctgaaggttt cagcaaacct tgtggcggtc tgctggcacc
    15481 ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg tcatcgccaa
    15541 cccgcagatt ttcagcgtca aaaccgtcga cgtcgccgca tcgctcacgc gtaactacca
    15601 gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat cgctgatccc
    15661 cgccagcgtt gaggtttacc acgacagcct gtgtcgaaaa atctggcgtg aggatgataa
    15721 atggcatgtc atttttcgcg cagacggctg ggagcagcat attaccgccc gctatctggt
    15781 cggtgccgat ggtgccaact cgatggtgcg gcggcatctc tacccggatc atcagattcg
    15841 taaatatgtc gccatccagc agtggttcgc agagaaacat ccggtgccgt tctactcctg
    15901 catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag acggttattt
    15961 tatctttggc ggtgcctatc cgatgaaaga cggtcagacg cgtttcacga cgctaaaaga
    16021 gaaaatgagt gcctttcagt tccagtttgg taaggcggtg aaaagcgaaa aatgcacggt
    16081 gctgtttccc tcacgctggc aggattttgt ctgcggcaag gacaacgcct ttctgattgg
    16141 cgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg cgctggatag
    16201 cgcagagatt ctgcgttcgg tattactgaa gcagccagag aagctcaata ccgcttacag
    16261 gcgcgccact cgcaaactgc gtttaaaact cttcggcaag atagtaaaaa gccgatgcct
    16321 gaccgcaccg gctttaagaa agtggattat gcgcagtgga gtggcgcata ttccacagtt
    16381 gaaagattat ccaacgcgct tcacatcgcc caccagcagg atgtaagaga gcgcgccaat
    16441 caacgcgacg gcggagatat aaaccagtgc aggtgcgaaa ccgtaaccct gcgccaggta
    16501 ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcaaagttaa acacgccgcc
    16561 ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg taatcgaagc
    16621 aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg gatcgttggt
    16681 gtagttcgca cccataatgc aggtggagat cagcaagccg cagataatcg gcgttttacg
    16741 cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc cagagagcag
    16801 gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt tcagcgccgt
    16861 gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc acagtgtaga
    16921 agccaccgca aattgcccaa gatagacacc gatcagttta cgatggaaca ccagtttcca
    16981 gtctttggct gttaacggct gacgtgcctc ttttttcacc ggcgcatcac catccaccag
    17041 accgccacca tcacgaatgt aatccagttc agctttgctg atgcctttgg tcaggcgcgg
    17101 cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac cggtgacaat
    17161 gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag tcagaaacgc
    17221 cagaccgaca aactgaccag acgtataaaa accaacggca gaagcgcgtt catgttccgg
    17281 gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga aaataccggt
    17341 tatcgcgcgc agaccaatta atgacattaa tcccgtggca aagccctgga ataacgtcgc
    17401 cactgaccag ccaaatatcg caataaaata agtcacgcga gaacctacgc gatctaaaaa
    17461 ccaaccgccg gggatctgac atagtgtata aagccaggcg aaggccgaaa atacatagcc
    17521 catttccgct ttggtaatac cgaactcttc ctgaatatgg gcggaagcca cggccaggtt
    17581 agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg tcagataacg
    17641 ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc tccagatttt
    17701 gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat ttttgtattg
    17761 ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc tacgctgtta
    17821 tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa ctcaataact
    17881 ttggcttcgt cgatttccac gcctaagccc ggtttcgtta atggtttaaa gaagccgccg
    17941 accatgctga agtcttcttt atttttcaca aagtcgagta actccgcgcc tttgttgtaa
    18001 tgaatgccca tactttgttc ctgaagtacc gcgttatagg aaacaaagtc gatatgcagg
    18061 caagccgcca gtgcaatcgg tccgagcgga cagtgcggcg caagggtcac gtcataggct
    18121 tctgccattc cggcaatttt gtagcattcg gtaataccgc ctgcgtggga gagatccggt
    18181 tgcagaatcg aaataccgcc tgcctccagc acacgtttaa aatcgaagcg tgagaacatg
    18241 cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca gtttcgggta gtattcggct
    18301 tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag ttctttaatc
    18361 agcactttcg ccatcggcgc gctgacgcga ccgtggaaat caagaccaaa ctcaatctga
    18421 ttgccaaaag cttcacgaat ttgtgccacg gtgttgaccg ccgcatctac cgcgcgggag
    18481 ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa gccgatttcg
    18541 cgtagcgttt taatgccgtc gataacatcc gccggacgat cgccgccgac ccaactgtag
    18601 gctttaattt tgtcgcgaac caggccgccc atcagttgcc agaccggcgc attcagcact
    18661 ttacctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat caggattgga
    18721 ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat gcgtgacggg
    18781 tcctgaccaa tcaaatagtc actcagctcg tggactgccg cttccaccgt gcgagcgcgg
    18841 ccttcaatca cgggctcgcc ccaaccgacc acaccttcat cggtttcaat tttcaggaac
    18901 atccagcggg gaggtaaccg atacgtggta attttggtga ttttcattgc actgcctctc
    18961 gatacgcctt aacaaatgct gctgcctgct gcgcagtccg ttctacggac tgcccggcgc
    19021 gatagagatc gctgcctaag cccgcccctg cacaacctgc gtctatccac tgcgccaggt
    19081 tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat accgctttta
    19141 atgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc gcctgcgcgc
    19201 ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcaca gccggggcag acggtcatgc
    19261 cgtagcccac cgcacggcgg atcacttcac tatggatatt gggcgtaacg atgagctgac
    19321 agcccattct ggcgagcgca tcgacctgtt caggtttcag taccgtacct gcgccaatca
    19381 acgccttatc gccgtacgca tcaacgatgg cgggaatgct ctgctcccat tgaggggaat
    19441 tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca tgcgccagcg
    19501 cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt tgccactgca
    19561 tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgctaccgc cgccacgtcg
    19621 caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc ggcgacaagg
    19681 gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc gccaatcaac
    19741 aaaccagaga gaaattcact gacctgttcg cggggaagtg ttcccagcac atgcgaggcg
    19801 cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccacgctc aaggccagcc
    19861 gcgaaggcat cggcagcgtt ttcctgcggc ggcaaacctg cgccaatcaa tgagtgattt
    19921 aacagtaaat gatgtaattc accggtcatt acggtgcgaa aatcgttgat ttgctggcta
    19981 tcggcctgca cccatttgca atgcgtaccg ggcatgacat aaagagagga aggagccaaa
    20041 gcacgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt atcatcatga
    20101 gaaacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa ttgttcgcca
    20161 atagatgaaa aacgggcagg aacagataaa tacggtgcaa ctttccagcc gacattgctg
    20221 ccgaccattc ctgccattac cactggcgta ttctcttcac gccagtcggt cgtcacttct
    20281 gctaacaccg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg
    20341 ctgtccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga tccccagtca
    20401 attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag ctggcgatca
    20461 tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccttatcgca tcgaacagcg
    20521 ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag gttcgttcaa
    20581 aaaccgcccg ctgcagcgaa ctgatcgcaa tgctcagttg ctgtaacacc gggttatgta
    20641 ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct
    20701 gattgttagc aatcatctcg ttcagcgccg attcaatctg cgccagatcg ctggaggtcg
    20761 cgcgctctgc tgcccaacgg gcaatagccg gctctaccag gtttcgcact tcgctcattg
    20821 cactgataag ccgtgggtca tagtcgtttt ccagcaccca ttgcagtacg tcagtgtcga
    20881 ggtaattcca ctggttacgc ggagccacaa acgccccgcg ataacgtttc atttcaatca
    20941 gccgcttcgc catcagcgaa cgaaatacct cacggatgat gttgcgcgag gttgcaaact
    21001 cctcacagag ttctgcctca gccggaagcg gcgagcctgg cacgtatttg ccgtgaacga
    21061 tctgtttacc cagcgtaata acgatgcgat cggttttatt gagagtcatg gagagtcctt
    21121 gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc aattttgtag
    21181 taccagcgtg atgacgttcg cgtttgctgt gcgcctaatg tagtacaaac ttatatagtt
    21241 gtactacaat ttagatcaca aaaagaacaa cccataagaa aatgttctac gtcgggcaaa
    21301 aatctgaaaa gggagatcag gcgctaaact ggggggaaag aagagtatgc tttcaacggc
    21361 ttagtactct tgtttaaagg attaatcatg aagttgaatc ttaagggatt tttcaaggct
    21421 gccagtttgt gttctttggc atttgcgttg accggatgta tcacctgggg attggtttcc
    21481 aatactgcaa gccgtccaca tgccgagcaa tggcggtcag acacgattaa gggtttatcg
    21541 ctggcagaag atagtaacgg tacgaaaggc tatgtttttg ttggtgaatc gttggactat
    21601 ctgttgacca ccggcggcga tgaggtggtt aaaatgctga acgatccggc aatccatggt
    21661 gaaagaatca ccgtttctga taacgcgaaa tttattttaa gcagcagtaa taagaatttc
    21721 tctggtgcta ttacgctcta ttacgactgg aacaacgaag aagataaggc actggcaaca
    21781 caatatggtt ttatttgtga tacccggcgt tgtacatgga tgctggacgg tttgaaaggc
    21841 tcaatccacc agaaaaataa aaaagcggat tactcaaacg tgatggtttt ccatcaaccc
    21901 tttacggtgg gattttatga gtacaaagca acagatggcg tcccgcgcgg cctcgttaat
    21961 gcgctgctgc cagtcactct tacgctggat atagtaacgt caccactcca gttcctgatt
    22021 ttatgcacga ccagaaattg ttaatcgctg cgggcaaacg cattcatgcc gtttgcccgt
    22081 tcactgatta attcagcaca tacttctcaa tagcaaatgc cacgccatct tcaaggttgg
    22141 atttggtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    22201 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    22261 cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc
    22321 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtgtat ttttctttca
    22381 cttcctgcgg aatacgcgca atggcctggt cgaggatggc gggttcatca atcatcatca
    22441 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaatgg
    22501 tggcaacgaa agattcatgc accgtgtagt agctgatatc acgattggcg gtgtacagcg
    22561 tgatgcggtc cagcgcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    22621 ggtagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta
    22681 ccagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca
    22741 ggtaattgtg aacacctgca taagggcgac ccgtcgttag cacgacattc acgccacggg
    22801 cgcgagctgc ggcaatcgca tttttaacgg caggtgaaat ggtgtgatcg ggcagcagaa
    22861 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccca ggtagattgg
    22921 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    22981 gggatgcaac gcgcattgcg tgctcccctt ttgcttagcg gaaaagtttc cctttcagca
    23041 gttccatgcc tgcggaaagc agatcgttat gggcttgtgg tgacacttca ccttgcggtg
    23101 agagcgcatc aataatcttc ggcaattgtt ctgccagtaa actggaagct gtactggtat
    23161 ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc gattccagtt
    23221 gctcgccgct aactgattga ttgctctgtt gattactcag ccaggttgag agaatggccc
    23281 ctaagccgcc actttgcagt ttttccaaca gcacttgaat gccgccctgt tcctcaaccc
    23341 agctcaaaat ggcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacgtcat
    23401 caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatc
    23461 tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct ccgccctttt
    23521 cttatagctt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa aacatgcaaa
    23581 ttcaatatat tgcaggagtc tcgtaggcct gataagcgta gcgcatcagg cacgctcgca
    23641 ttgttaacgc cattaaatat cgatattcgc cgctttcagg gcgttctctt cgataaacgc
    23701 acggcgcggt tcaacggcgt cgcccataag cgtagtgaac aactggtcgg cagcaatcgc
    23761 atctttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg tttcccacag
    23821 ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc cgcgacggga
    23881 ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac gacgttcgcc
    23941 acgttcgata aatgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat
    24001 acgacgatat tcgccgccag tgataaattc gtgatccagc ggatagtcag tatccacacc
    24061 gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg tatggacatc
    24121 aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca gcgcgttcac
    24181 ccagcgggta acggtctgct catcagagag atcggcttcc gtcagcgtcg gctgatagat
    24241 aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca ttttctgcgt
    24301 cgcgttgtac tcagacacca gtttctctaa cgcttcgcca gccagcgccg gtgcactggc
    24361 gttggtgtgc agcgttgcgc catccagcgc gatagagatc tggtactgat ccatcgcttc
    24421 gtcgtcttta atgtactgtt cctgcttacc tttcttcact ttgtacagcg gcggctgagc
    24481 gatgtagacg tgaccacgtt caacgatttc cggcatctga cgatagaaga aggtcaacag
    24541 cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata
    24601 acgcagtttg tccgggttgt actcgtcacg accgatacca caaccaagcg cggtgataag
    24661 cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga cgttgaggat
    24721 tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc
    24781 gcccgcggag tccccttcca caaggtacag ttcggaaagc gccggatcgc gttcctggca
    24841 gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc gggtcatttc
    24901 acgcgcgcga cgcgcagctt cacgggcacg ggcagcatcg ataattttgc cgaccacgat
    24961 tttcgcgtcg gttgggtttt ccagcaggta ttccgccagc agttcgttca tctgctgttc
    25021 aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgtgagg agaatttcgg
    25081 gtccggcact ttcacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc
    25141 gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcgttca gagtacgggt
    25201 catcgccgca cggaaacctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt
    25261 aaagcagtag atgttttcct ggaagccatc gttccactgc aacgccactt cgacgccaat
    25321 accgtctttt tcggtggaga agtagaagat attcgggtgg atcggcgttt tgttcttgtt
    25381 cagatattca acgaacgcct tgatgccgcc ttcatagtgg aagtggtctt ctttgccgtc
    25441 gcgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg
    25501 tttcgccaga atgtcatatt cgaactcggt cacattggtg aaggtttcaa ggcttggcca
    25561 gaaacgcacc atagtgccgg ttttttcagt ctcgccggta accgccagcg gggcctgcgg
    25621 tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctggataa ccagctccag
    25681 tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt
    25741 ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc
    25801 cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccatcat cctgtacgga
    25861 gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc
    25921 gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt
    25981 gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat
    26041 actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat
    26101 ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa
    26161 cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg
    26221 caagcagccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac
    26281 accgccgccc gctttcacgg gtgaggaact ctccttgcgc cagacgtaag gcgcacatca
    26341 acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc
    26401 gtaagtccgc tttatgcggg ccgtgcgcgg tgtaggttag ctggcgatcg cgttcaaaat
    26461 tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg aaagagaaag
    26521 tcagagaaaa ctcagggaga aattgcttac aggtatcagc catatcggct gcgataccgg
    26581 cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt tctttatccc
    26641 acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc tgcttgagca
    26701 atcgcttgag attgctccag gcggtgaaaa atccgggttc gttgtgaaag catccccagt
    26761 cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta
    26821 tcagctgcat tggcatcagg tgcgccagct ccgcgacttt atgcccgtct gtaccgtcaa
    26881 tacggacttt gctgtcgcct tgtttgtctt tggttaaacc aatcgccgtc tcgcgctctt
    26941 cgccctgtaa tcgcccgtgg agaacaaacg cctcctgctc gtggcgaatg acgcgaccaa
    27001 tctgcaaact gcgaaacgcc cgaccgtggc cgagcgtata gatggcttcc agcacgctgg
    27061 ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataag gcgagatccg
    27121 cggtttcaat gttgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat
    27181 tggcatgaca acataagccg cgctctggct ggccgcatct tcaatctgca cgctggaaac
    27241 cgaatcggtc agcatcatgc ggacgttttc gcatttcagt gcgttcagaa catccagcac
    27301 atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc
    27361 ttccgcttct tcctgttctg ggttgttggc ggtgattttc agctggtttt cgctgacata
    27421 cagacgcacg ccgcggaatt tctcgttaga gagaattgcc gcacgggcaa acgcctgctt
    27481 gagcagatca cagccagctt ccagatgctt gtccgggttc ttcggcagaa cgcgacggta
    27541 atccgggaag cgaccatcca ccagtttcga ggtgaagata aagtcgccaa cgtgggcgcg
    27601 aatgttgttg ctgccaatct gcacgcgcag cggattgtcg ccgccgtcga gcatacgcat
    27661 cagttcaatc acgcctttac gcggcacgat caccgaatgg cttggcaaag attgaccaat
    27721 tggcattgaa cagactgcca gacggtggcc gtcggtcgcc acggtgcgca gttcttcacc
    27781 ttcggtttca aacagcatac cgtttaagta atagcgaacg tcctgatgcg ccatagaaaa
    27841 ctgggtcgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct
    27901 ctgccagtca tcgaggttcg ggaaatctgc cgctggcagg gtagacagcg aaaaacggct
    27961 acgcccggag cgcaccagca tccgctcacc ttccagctgc acggcaattt ccgcgccttc
    28021 aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg cccctggttc
    28081 gtgcggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag taccggtcag
    28141 cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg
    28201 accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt
    28261 aaatttcata ggtttacgat gacaatgttc tgattaaatt agaaaaatct tctttgatat
    28321 cgtggctctc ttcacgcaac tgctcgatct tccggcaggc atgaagcacc gtcgtgtggt
    28381 cacgaccacc aaaggcatcg ccaatctccg gaagactgtg gttagtcagc tctttcgcca
    28441 gcgccatcgc catctggcgc ggacgggcca ccgagcggga tcgacgcttg gaaaggagat
    28501 ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca
    28561 gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg
    28621 cccgtccggt aaagttggca ttggcaatga cgcggttcag cgcgccttcc aactcacgta
    28681 cgttagatcg tagacgcttg gcgataaaaa acgccacttc gcccggcaaa cgaatgtcgt
    28741 tttcgtcggc ctttttcatc aggatcgcca cgcgggtttc cagctctggc ggttcgatcg
    28801 ccacagtcag tccccagccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt
    28861 tcggatagcg atcggaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg
    28921 tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca
    28981 gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcaggg
    29041 ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct
    29101 tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc aaacccgtgc
    29161 cgccataaag gaacaacggg ttataagcac cgccagggtt atctgccacc tggcgagccg
    29221 ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt
    29281 ttacgttaga acgataggtc ggttctgccg gagccgggac gttatcccaa cccgagcgcg
    29341 tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc
    29401 tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct
    29461 gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt
    29521 cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca
    29581 gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca
    29641 atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag
    29701 tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgca
    29761 tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg
    29821 atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt
    29881 cccgatttat ccacaggatg atccagaact cgctaagtgt aaacgatcct gccgcgggcc
    29941 gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat
    30001 cgatctaata aagatccagg acgatccttg cgctttaccc gtcagcccgt ataatcctcc
    30061 acccggcgcg ccatgctggt tttcactggt gtgaggccgt acgttttccc cgcgaaaagg
    30121 tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggagtgtaca attattacaa
    30181 tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc
    30241 caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca
    30301 accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa
    30361 tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa
    30421 gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa
    30481 ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta
    30541 ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga
    30601 aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc
    30661 gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg
    30721 ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc
    30781 gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct
    30841 actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg
    30901 caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc
    30961 tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta
    31021 acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc
    31081 tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca
    31141 acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa
    31201 ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt
    31261 gagcaagccc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg
    31321 ctggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc
    31381 ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg
    31441 ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac
    31501 acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac
    31561 gtgcagaacg ctggcgagaa accgctggaa atctccacct ttggtcagtt gaagcaatcc
    31621 atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgt
    31681 ggtgcggcgt actccacgcc tgacgagaag tacgagaaat acaagttcga taccattgcc
    31741 gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcaatgct gcaacagtat
    31801 ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac cgctaatctg
    31861 ggtaacggca tcgccgctat cggctataaa tctcagccgg tactggttca gcctggtcag
    31921 actggcgcga tgaacagcac cctgtgggtt ggcccggaaa ttcaggacaa aatggcagct
    31981 gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat ctctcagcca
    32041 ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc
    32101 atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc
    32161 atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat
    32221 gacaaacagc gcatcagcca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg
    32281 ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg
    32341 ctgatgggtt ccgttgaact acgtcaggca ccgtttgcac tgtggatcca cgacctgtct
    32401 gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag
    32461 aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac ctttatgccg
    32521 gtcatcttca ctgtgttctt cctgtggttc ccgtcaggtc tggtgctgta ctatatcgtc
    32581 agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc
    32641 ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg
    32701 tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcacatc cgccacacaa
    32761 agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac gcctccggga
    32821 cgtggcggcg ttggcatcct gcgcatctcc ggcctcaaag cccgtgaagt tgccgaaacc
    32881 gtgctgggta aactgcctaa gccacgctac gccgattatc ttccgtttaa agacgccgat
    32941 ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc
    33001 gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct gctgttaaaa
    33061 cgcattctga ccattcccgg cctgcggatt gcccgccctg gtgagttttc cgaacgcgca
    33121 tttctgaacg ataaactcga cttagcccag gccgaggcga ttgcggatct tatcgacgcc
    33181 agctcggaac aggcggcccg ttcggcgcta aactcgctgc aaggcgcgtt ctccgcacgg
    33241 gtcaaccatc tggtggaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat
    33301 ttccccgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca gctcaataac
    33361 gtaattgccg atctcgacgc ggtacgcgcc gaagctcgtc agggtagttt gttgcgcgaa
    33421 gggatgaaag tggtgattgc cggacgtcct aacgccggta aatcgagcct gctgaacgcg
    33481 ctggcgggcc gtgaagcagc aatcgtaacc gatatcgccg gaaccacgcg tgacgtgctg
    33541 cgtgagcata tccacattga cggaatgccg ttgcatatca tcgataccgc cgggctacgt
    33601 gaagccagtg acgaagttga acgtattggt atcgagcgcg catggcagga aattgaacag
    33661 gcagaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga tccggcagag
    33721 atctggccgg aatttatcgc ccgtctgcca gcgaaactgc cgatcaccgt ggtgcgcaat
    33781 aaagccgata tcaccggcga aacgttgggg atgagcgaag tgaacggtca cgcgttaatt
    33841 cgtctttcgg cgcggactgg cgaaggcgta gatgtgctgc gtaaccatct caaacagagc
    33901 atgggctttg acaccaatat ggaaggcggc ttcctggcgc gtcgtcgtca cctacaggcg
    33961 ctggaacagg cggcagagca tttgcagcaa ggtaaagcgc aactgttagg tgcctgggcg
    34021 ggtgaactgc tggcggaaga gttgcgcctg gcgcagcaga acttaagcga gatcaccggg
    34081 gaatttactt cagacgacct actggggcgg attttctcca gtttctgtat cggtaagtaa
    34141 ccgcgcttgc gaagccgcat tctgactgtc agatgcggct tcgcttcatt gttagcactc
    34201 ctgttattcc tcaacccttt tttaaacatt aaaattctta cgtaatttat aatctttaaa
    34261 aaagatattt aatattgcgt cccgaacgat tgtgattcga ttcacattta aacaatttca
    34321 gaatagacaa attccttgag tattatactg tagcctcgtg tctttcgagg ataagtgcat
    34381 tatgaatatc ttacatatat gtgtgacctc aaaatggttt aatattgaca acaagattgt
    34441 cgatcaccgc ccttgatttg cccttctgta gccatcacca gagccaaacc gattagattc
    34501 aatgtgatct at
//
LOCUS       DAZKHK010000044        33860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKHK010000044 DAZKHK010000000
VERSION     DAZKHK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.044"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..898)
                     /locus_tag="ACHOS5_002113"
     CDS             complement(<1..898)
                     /locus_tag="ACHOS5_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9776929.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFASTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTRFV"
     gene            complement(976..2199)
                     /locus_tag="ACHOS5_002114"
     CDS             complement(976..2199)
                     /locus_tag="ACHOS5_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9776930.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2225..2614)
                     /locus_tag="ACHOS5_002115"
     CDS             complement(2225..2614)
                     /locus_tag="ACHOS5_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HFP9776931.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGGWRKYTGHFHTV"
     gene            complement(2631..3587)
                     /gene="arcC"
                     /locus_tag="ACHOS5_002116"
     CDS             complement(2631..3587)
                     /gene="arcC"
                     /locus_tag="ACHOS5_002116"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9776932.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISIAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3580..5004)
                     /locus_tag="ACHOS5_002117"
     CDS             complement(3580..5004)
                     /locus_tag="ACHOS5_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9776933.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPVDATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(5001..6560)
                     /gene="fdrA"
                     /locus_tag="ACHOS5_002118"
     CDS             complement(5001..6560)
                     /gene="fdrA"
                     /locus_tag="ACHOS5_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9776934.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALALAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNAQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(6655..7515)
                     /locus_tag="ACHOS5_002119"
     CDS             complement(6655..7515)
                     /locus_tag="ACHOS5_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9776935.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTQGLLRTPRFCVALENATLWQS
                     PFVGRMLTFEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7521..8189)
                     /locus_tag="ACHOS5_002120"
     CDS             complement(7521..8189)
                     /locus_tag="ACHOS5_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFP9776936.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWITDSKAIIA
                     GLEG"
     gene            complement(8446..9255)
                     /locus_tag="ACHOS5_002121"
     CDS             complement(8446..9255)
                     /locus_tag="ACHOS5_002121"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP9776937.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9517..10281
                     /gene="udp"
                     /locus_tag="ACHOS5_002122"
     CDS             9517..10281
                     /gene="udp"
                     /locus_tag="ACHOS5_002122"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP9776938.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(10316..11806)
                     /locus_tag="ACHOS5_002123"
     CDS             complement(10316..11806)
                     /locus_tag="ACHOS5_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HFP9776939.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(11819..12325)
                     /locus_tag="ACHOS5_002124"
     CDS             complement(11819..12325)
                     /locus_tag="ACHOS5_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HFP9776940.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVAMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(12342..13316)
                     /locus_tag="ACHOS5_002125"
     CDS             complement(12342..13316)
                     /locus_tag="ACHOS5_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HFP9776941.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASWPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPNGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRSDAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVNG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(13341..13970)
                     /locus_tag="ACHOS5_002126"
     CDS             complement(13341..13970)
                     /locus_tag="ACHOS5_002126"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP9776942.1"
                     /translation="MPFNTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYRPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(13960..14964)
                     /locus_tag="ACHOS5_002127"
     CDS             complement(13960..14964)
                     /locus_tag="ACHOS5_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP9776943.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQSAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEHGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(14988..15755)
                     /locus_tag="ACHOS5_002128"
     CDS             complement(14988..15755)
                     /locus_tag="ACHOS5_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFP9776944.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            15972..17399
                     /gene="rmuC"
                     /locus_tag="ACHOS5_002129"
     CDS             15972..17399
                     /gene="rmuC"
                     /locus_tag="ACHOS5_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP9776945.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQKIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17494..18249
                     /gene="ubiE"
                     /locus_tag="ACHOS5_002130"
     CDS             17494..18249
                     /gene="ubiE"
                     /locus_tag="ACHOS5_002130"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP9776946.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18263..18868
                     /gene="ubiJ"
                     /locus_tag="ACHOS5_002131"
     CDS             18263..18868
                     /gene="ubiJ"
                     /locus_tag="ACHOS5_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP9776947.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALVDLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQCYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            18865..20505
                     /gene="ubiB"
                     /locus_tag="ACHOS5_002132"
     CDS             18865..20505
                     /gene="ubiB"
                     /locus_tag="ACHOS5_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP9776948.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20584..20853
                     /gene="tatA"
                     /locus_tag="ACHOS5_002133"
     CDS             20584..20853
                     /gene="tatA"
                     /locus_tag="ACHOS5_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP9776949.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            20857..21372
                     /gene="tatB"
                     /locus_tag="ACHOS5_002134"
     CDS             20857..21372
                     /gene="tatB"
                     /locus_tag="ACHOS5_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP9776950.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETVHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21375..22151
                     /gene="tatC"
                     /locus_tag="ACHOS5_002135"
     CDS             21375..22151
                     /gene="tatC"
                     /locus_tag="ACHOS5_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP9776951.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22193..22975
                     /gene="tatD"
                     /locus_tag="ACHOS5_002136"
     CDS             22193..22975
                     /gene="tatD"
                     /locus_tag="ACHOS5_002136"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP9776952.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(22972..23460)
                     /gene="rfaH"
                     /locus_tag="ACHOS5_002137"
     CDS             complement(22972..23460)
                     /gene="rfaH"
                     /locus_tag="ACHOS5_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP9776953.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23627..25120
                     /gene="ubiD"
                     /locus_tag="ACHOS5_002138"
     CDS             23627..25120
                     /gene="ubiD"
                     /locus_tag="ACHOS5_002138"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP9776954.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25166..25867
                     /gene="fre"
                     /locus_tag="ACHOS5_002139"
     CDS             25166..25867
                     /gene="fre"
                     /locus_tag="ACHOS5_002139"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP9776955.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26139..27302)
                     /gene="fadA"
                     /locus_tag="ACHOS5_002140"
     CDS             complement(26139..27302)
                     /gene="fadA"
                     /locus_tag="ACHOS5_002140"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP9776956.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARDLGLKPRACVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27312..29501)
                     /gene="fadB"
                     /locus_tag="ACHOS5_002141"
     CDS             complement(27312..29501)
                     /gene="fadB"
                     /locus_tag="ACHOS5_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP9776957.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAIAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAAMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLNYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSHAKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29691..31022
                     /gene="pepQ"
                     /locus_tag="ACHOS5_002142"
     CDS             29691..31022
                     /gene="pepQ"
                     /locus_tag="ACHOS5_002142"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP9776958.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31022..31636
                     /locus_tag="ACHOS5_002143"
     CDS             31022..31636
                     /locus_tag="ACHOS5_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP9776959.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31675..33126
                     /gene="trkH"
                     /locus_tag="ACHOS5_002144"
     CDS             31675..33126
                     /gene="trkH"
                     /locus_tag="ACHOS5_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP9776960.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33138..33683
                     /gene="hemG"
                     /locus_tag="ACHOS5_002145"
     CDS             33138..33683
                     /gene="hemG"
                     /locus_tag="ACHOS5_002145"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP9776961.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8030 a         8681 c         9012 g         8137 t
ORIGIN      
        1 atacaaatcg tgtcattaat tgtgggtttc ttaaatattc tatgcaccac tgacaggctt
       61 tgcccatacc atcggaacga gcggcaagaa ataacttggt ggcgtgttta tgttcgcgca
      121 ttggcttttt aagcaattta ccgctgttca ataacggcaa tgccagatga tgtggaatat
      181 aaccaatacc gacgttttgt tctattaagg cgattgccgt agcgaagtca ggaacaaaaa
      241 tgggtttttg tccttccaac aaccaggctt gcatcggggg gaagttaacg gaggtatcgc
      301 ggatacaaac cgctggataa tgacgcaatt cactgttttc taatggatgc cgcttcgctg
      361 ccagtgggtg gagtgggctg acgacaaaat cccattccat ttgtccgacc ggttcgctaa
      421 tgatgccttc actgcttggt actgcatgag gagcaccgta gaccagatca gcacgcttac
      481 tgtacagcgc atcccagcag ccgttgtata cctcaagatc taccgtcagt gttgttgatg
      541 cgaattgccg ttcaaaatct cgaataaatg ccaccagcaa atcaccagga ataatattgt
      601 ttaccgcaat tgtcagttca cgctcaatac catcgtgaat cagcactgtg ttgcgcttta
      661 atgcttccag gtcgccaaga atagttttgc tatgttcgat gaagtaactt cccgctggcg
      721 ataattcaat atagcgtccc ttacgaatga acagttcaac gcccaggctc tcttccgctt
      781 tcttgatagt gtaactgatg gcggaaggga ctttatttaa ctgctcggct gcggtggtaa
      841 tgctctggtg tctggcgacc agatgaataa cacgaagcgt ttcgtctgag atgaacataa
      901 ggctccctgg tcagtggtaa agtggctgaa tctctccaga ttcagccgta atcattgcct
      961 gagtataccc aggaattagc ggtgttgact tgcccgactg agaatcgcat acgcgataca
     1021 tgccaccaga atcgagtcta ccgaagggat cgtcgtcagg gtgaataagc cttttacggt
     1081 cataaagcca accgctgcgc ctatgatcca tgctgcgaat gtcagcactt taatctgtga
     1141 ctcctgggct aacactgact cttgatagcc attacggcga taaaggaaaa agtcagcgat
     1201 ataaattccg gcaacgggtg gcgtggcgat acccaggaac aacagaaacg gaataaacca
     1261 cgccataata tccatactgc ctacgatggc ggaaaggata cccagcgcca cggtaatctg
     1321 ccatttggga aagcgggtaa gcagtgtgga aaccaccagc gtgccctgga acatattgcc
     1381 cgcgttaccg gtgacgcagg cgaaaatcaa caacagcgcc gcaggcagaa ccgcaccgaa
     1441 taccgccatc gcacccagta aagatccctg accactaatg gcgctcggcg ttgcacccgc
     1501 ccagtagagc agagggtagg caaccagaaa cgtcacgcct gcggcaatca gcgcatgttt
     1561 gcggttatgc acaaaactac cgaaatcagg cagtgtggca accagcacaa taatggtgcc
     1621 aacaacggtg gaaacggcga cacctgtatt catctccaca atcgccgctg gtactgccac
     1681 ttcggtatgg gttgccacat agaggatgta acacagcacc agcgcgataa ccggcacggc
     1741 aacctgggca atttttccca gaacggcaaa gccaaatgcc gtagaggcga caaaaatgac
     1801 acagccagcc gccactaaca gtgccagtgg aacttccagt ccatgttgag ccagtaagtc
     1861 atgtaccgaa tgaccaaaca tattggcggt aacggcaatc cagccgaaca gacttatcgc
     1921 catcaaaatg ttaatggcga tggccccttt ttcgccaaac gaatatttca caatgccata
     1981 tgttggcagg cgggcttttt ctcccacgct aatggtgatg gctgaaagta aggtcagaat
     2041 gacgccgcct aacaatacga cgctaattaa actgccgctg gaaagttgat tgccgaggct
     2101 tgaggatgac aacagcacag gtgtgacggc aatacccagc aaaatcatgg caatacgcaa
     2161 tcctgatgcc gtattcagat tctcttcttt tttacgcata gtatgttccc tttataaatg
     2221 cgcgttacac agtatgaaaa tgcccggtgt atttgcgcca gccgcccagt tctgtaattt
     2281 cccgttggcc ttcggtcagc cagttttctg ccagtacgcc cagcacttcg ctaccatcgg
     2341 caagtttgat tttgccaatt gccagcccag cgggttcgct cattaacaga gcggcaaaac
     2401 tggccagcgg taattgccaa atttctacgt cgacgtgtgt tcctccttca ttcacgcgaa
     2461 tcattcccgg atggcgatca ttaatactcc acaaccggta atgggcgtca gttcggtctt
     2521 cacggacaaa aataccgccc gctttttgca tatttgggtt gagttccagg ccgcgcatca
     2581 gcgttccgtt aacggctaaa agcgttgtag tcattgtgtt ctccagagaa ttaaagggtt
     2641 atccatgtac cgctctgatg cgccagggct gcttttatgg tttgcggtga agtaatcagg
     2701 ccgctggcct gtttgccttg ttgctggctg gcattgatga aatcaacaat ggcttccact
     2761 ttgggttgca tactgccgac accaaactga ccttcccgca acagcgtttg cgcttcggca
     2821 atgctgatag cgtcgagcca ctgttgttgt ggtgtaccaa agttaatcgc cactttttct
     2881 acaccggtgg ggatcaccag cagatctgcg cctaactgac tggcgagcag cgcggaggcc
     2941 agatctttat cgatcacggc ttctacacct ttcagttgtt gcccgtctcg caccaccgga
     3001 ataccgccgc cgccgcaggc aataaccaga tatccctggc gcaccaggtg agcgatggtg
     3061 tcgtgctcaa taatttccag tggtgcagga gagggaactg tacgccgcca accacgcccg
     3121 gcgtcctcca tcagcgtcca gccgagttgt tgttggcgtt gttgggccgt tgcttcatcg
     3181 agaaacgcgc caatcggttt actggggctg gcgaaagcat cgtcatgtgg gctgactcgc
     3241 gtttgtgtca ccagggcaat taccggtttg tttatgccac ggcgagcgag ttcgttatgc
     3301 agcgctttct ggaacatgta gccaattgca ccttgtgtat cacccacggc gtaatcaagt
     3361 ggaaccggag aaacttcgtt actggcgagt tcagagcggc gcagaataaa gcccacctgc
     3421 gggccattac cgtgggttag caccaggtcc catccggctt caaccatatc aacgatatgc
     3481 tgcacgcttt ccatcactgc aacatattga tcgggaatac tattgcgttg ctcgtcctga
     3541 atgagcgcat tgccgccaac cgcgacgaca gccagtggct taaccatgat ggttctcctt
     3601 tgcgacgatc tccgccagtg cacgtacagc ggcgacaaaa caggccatcg gcgcagtggt
     3661 gatcccggca ccaatttgcc cgacccccgc ttgtttgtgg gcgatacccg tattgatgac
     3721 cggtaaaatg ccgcgatcgg tcactttacg cgcatcaatc cctgctgcgg tgggggcaaa
     3781 attgagggct ggaagtgtaa atgcggggtt accgccgagg gtgatggctt gcatccgacg
     3841 gctgttattg gtggcatcga caggggtgcc tcccacaaat ttgacgatcg ccggagacga
     3901 cgccatcgcg aagccaccca cacctgcggt ttcagtaatt gcgctatcac ccaggtcagc
     3961 agcagcgtcg tcaacgccaa atccggggaa gaacagacct tcaacggcat tggcaggagc
     4021 ctggaaccag cgatcgccag taccggaaag ccgaatacca aaattcaccc cgttacgcgc
     4081 catcaccgta accattgaac tgaacggtac attggcggcg gcatccatca ttgctttaca
     4141 ggcagccatc gatagattga ggaagaagtg atcgttaccg gtgataaatg ccacgacgcg
     4201 ctggatatgt tcctgtggaa gagagcaggc cagcaatgcc gggagcaggc gcttaatcag
     4261 caaaccggta gcggcagcat tgcggttatg aacttcatcc cccatatgca gcgcctgcgc
     4321 cattaatggc ttgagttcca gctcgccgtg ttgtgcgatt gctgctttca ttgccggcgc
     4381 cagttcatcg cgcatccagg ccagacgatt gagaacttca tcgttattgg cgccaaaacg
     4441 gagaacctta cccagacctt cattgaaatt gctaaaagta cggtggccgt tggttttgtt
     4501 ttctaccacc cataacggca tcgacgggct gatgatgcct gccattgggc caaccgcgtg
     4561 ataatgatga caaggggcaa ggtcgatttc gcctgcgttt atttggtttt cagcgtcttg
     4621 ctggctggtg gcccaacctt cgtacagcat ggcaccaata atcgcccctt ttaccggacc
     4681 acacatctct tcccaggcaa tcggcgggcc agagtgcaaa atacgttttt gttcagccat
     4741 tgctggaatg gcctctttag cgagcatgac atccaccagc acgggctgtg cttcgagata
     4801 acgtgtcatg gcgatttggt tggcattttc taccagagga tgacgtagca acgatgccag
     4861 atccaatcca gcgtcaatat cgccttgtgc tggtggctgc cagttgagtg caattgcgct
     4921 tcccccggca tgttgaatat tgtcagcaaa actgtgtagt cccgcgttca caaccttcaa
     4981 aggctgatta aataaggtat tcatgcgttt agcccctttt tctgcaactg agtctgtgca
     5041 acggtgctgg cccatagcgc cgcctgtgca ttgctgctgg cgatgatgac ccctgcattt
     5101 tgtagtgcgc ttatctgctg actgcgtatt tgcggatcag cttccgttcc gcaaacgtgc
     5161 gcaatcagca acggtgcgtt attcatgtcg atgtgtgaaa gttggtccag taattcgctg
     5221 gcaggcgtcg tggaagcgcc ataaccaagt accaggtcga ataacacgac ggcagtgtgt
     5281 gagtcgttga gttcgttgag cagacgttga ttgcgcagag ttgggtcgat cattggatgc
     5341 ggtttgccac gggtgaaatc atcgtcgccc atatcgatca acgtgtgatc ttcgctttgc
     5401 cagatatttg ccagagcacg attacccgca actggcgtat tggaagctgc gctgaatccc
     5461 ttttgttggc agataagttg cgcttcgtag cagaaggttc ctccggcgaa aacaccgcgt
     5521 atcgcctggc gatgggctgg caggcgctgg caggcgtttt gtaacattgt gagatcgtcg
     5581 cagggtacat cggtttcgac agccggaagt ggctgagcat tcagtaacgc cacagcgatg
     5641 tcggcggcgc tggcaagtgt ggtggccgca gtgatattgg gacgtgccag atcatgcgga
     5701 ttcgccccga ggaaattcac cactacgggt tttccacaag cctcggcacg ctccagaata
     5761 gtccgggcaa cgatcggtga aggcggtttt gatataagca caatcacgcg agtttcatcg
     5821 tcttgcgcca gcgcatctaa agcgaagagc atcgatattc caccaatctc ttcactgaga
     5881 tcatgtccgc ctgttcccaa cgcctgagaa atacccgctc cgagctggtc gattcggcaa
     5941 gtcacttcct gtagcccggt gcctgacgcg ccaataacac caattgctcc gcgtttcacc
     6001 acattagcga agcctaacgg aatgccgtta acaatggcgg ttccacagtc aggtcccatt
     6061 acgatgcgct gacgttctcg ggcaagcgtt ttgatggatt tttcctgccc gatactgacg
     6121 ttatcgctga acatcatcac gttcattccg aggtttaacg ctttaattgc ttcggcggcg
     6181 gcgtagtctc ccggtacaga aatgagtgcc aggttggctt cgggttcggc ttccagtgcc
     6241 atttccaggc tgaccttttc cggtgtttta atgccactat ccgtttcatc atcgggtttg
     6301 ctggtcagac gttgctgggc cagtgccagt gcttcattgc aaatatcttc ttcacccatg
     6361 actgcaatca ctaaatcatt gggactggcg ttgatttcgt tacccagacc cgcatcgcgc
     6421 aattgttcga gattattggg cgttcccatg acgaccgagg cctgttgaat accgggcaac
     6481 ttgctgattt gcgcggaaat ctgcatcaga gaaactgagt cctgataaag attactgaaa
     6541 actttatatt taactttcat gctgttacgc ctgtttcatt ccgcccgata gcaaaattcg
     6601 ggcaaagcat ccccgtgtag ccccataaac gggctacggg agaaaaggtt gaaatcaatt
     6661 catggtgttc agggttcgaa agccatgcag cattcctgca aggcaatcta ctcctgacgt
     6721 tgcgccaaat tgcatgacct gttctgcaca ggatgcgatt gtcattgccg atgcgctccc
     6781 aaccagttgt gcgagtaact ggcaaatcgg ttctgaaaaa tgtccctgaa gcgcacgctc
     6841 cagataatga cgactgatat ccgtggtgtt gtgcgcatgt tgatcaattg cctgctgcaa
     6901 ccggtattga tgatcggcaa gtggtgcagg gagttgccat aaccagagcg ctgccagata
     6961 gcccaacaaa tagtcatctc catcgggcgt taagccttta ccaaagccga tcagttgtgg
     7021 cacctggcgt tccagcgcat ctggatgctc cataatcgct aatccctggt gcgtgagact
     7081 atccggcagg tgcaatgtgc tttgaacgtt atgttccaga caaaataaaa gccgttgcga
     7141 tgccatcgtt gggtagtgct ggaaaaccaa agggaatgcc tcaaatgtca gcattctccc
     7201 cacaaatggc gactgccaga gcgttgcgtt ttcgagcgcc acacaaaatc gaggtgtccg
     7261 cagtaagcct tgggttaact gaatgggatc tgaatgggca atctctcttc gccagtccca
     7321 acattcaggt agccagactc tggcagcatc agggagattc agatacgttt gcgcacacaa
     7381 cagagtgatg aatgttcccg cgtcagaata cagatttacc gcatgtttaa aagcgctatg
     7441 tagacgtaaa tcttgcgttt cattctgcgg gaaaagatat cccaacgacg atgcgcaaag
     7501 cgtaacctgt accatcggcg ttagccttcc agacctgcaa taatggcttt ggaatcagta
     7561 atccaaccga aaatggcacc ttgtgctttg atcatctcaa gggcgtattt ctggaattcc
     7621 gggaaatagg agccgacaca atcttccggg ataatgcatt catagccacg atcatttgct
     7681 tcgcgcacgg tggtggttac gcaaacttcg gttgttacac cacaaactat cagcgtcttg
     7741 ataccgtggt tttgcaaaat cagatgtaag tcggtttgat aaaaagcgcc tttgccaggc
     7801 ttatcaatca ccggttcgcc tgccaccggg tacagttcag gaatgatgtc atgacctgct
     7861 tcacctctga ccagaatgcg tcccatcggt cctggttcac caataaaggt ttttccgccg
     7921 cgcgtcagtt tggctggcgg gcagtcgctt aagtctgcac gatggccttc acgagtatga
     7981 ataaccataa ttcctttttg acgtgcggct gccagcacct ctgtacatgg tgcgatagcc
     8041 gtgcgtacca gtgaaacatc attgcccagt gcctcaccga agccccctgc ttcgacaaag
     8101 tcacgctgca tgtcgatcat gaccagtgca gttgtgcgcg gatcaaaagg taattcaaag
     8161 ggttgagcct gaaaaatgga ttgcgtcatg gtaaatctcc tgtctgatgt ttaaacgatc
     8221 cagttgttct gagaacgagt attcaggaga aaagcggcac aaaacaggaa aaaagtttgc
     8281 cgcacagcaa cgaaattttt gcattcagca ggtggtgtga tgacgaatgt gaagggagtc
     8341 aaagagtggc atacagcaac aaattttttg atgctgtatg gttttgagat taaaaaaatc
     8401 gttgaaattg tgtgagctta atcatcagat ttagcgacta tggcgttact ttttaacacc
     8461 atactgtgta aaccattcca acattcgctg ccagccgtct ttcgcagact cggcatgata
     8521 gctcgggcga taatcggcgt tgaacgcatg ccctgcatcc gggtacacga taatctctgc
     8581 tttcgcgttt gctgcccgca gcgcctggcg catcgtttcc acgctctctt gcggaatgct
     8641 gttatcctgg ccgccatata agccgagaac cggcgcgtta agatcggttg cgatatcaac
     8701 aggttgtttc ggtgaattca gcgacttatc gcccgtcagt ttgccgtacc acgccactgc
     8761 ggcttttagc tgtggattat gcgcggcata cagccaggtg atacgtccac cccagcagaa
     8821 tccggtgatc attaaacgat gaacatcgcc gccgttgcgg gaagcccaac tggcgacatg
     8881 atcgagatcg gccagcacct gcgagtcagg cacttttgct accagaccgc taagcaacgt
     8941 ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag gtgcgatagc
     9001 cagatacccc tccaacgcca gacggcgaca aatgtcgcgg atatgttcat gcacgccaaa
     9061 aatttcctgc actacaatga ccactggcag tgggccatcg ctttgctttg gtctggcatg
     9121 gtaagcaggc atgttatccc cttgtgaagg gatagaggtg aagcccgcca caatcgcgtc
     9181 gtccggagtc tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa atccagattg
     9241 ttgtgttgtt gccatggtat tctccgtacc cttataaaaa tgttgcgcaa tgttaactat
     9301 agtcagcatg caacaaatca cattgcctga atcggctcat cttttatgca gtcctgcaga
     9361 atgaagagtg atttatgtga tttgcatcac ttttggtggg taaatttatg caacacattt
     9421 tcgtcatggt gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt gtctttttgc
     9481 ttcttctgac taaaccgatt cacagaggag ttgtatatgt ccaagtctga tgtttttcat
     9541 ctcggcctca ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc tggcgacccg
     9601 gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg ttaagctggc atctcaccgc
     9661 gaattcacca cctggcgtgc agagctggat ggtaaacctg ttatcgtctg ctctaccggt
     9721 atcggcggcc cgtctacctc tattgctgtt gaagagctgg cacagctggg cattcgcacc
     9781 ttcctgcgta tcggtacaac gggcgctatt cagccgcata ttaatgtggg tgatgtcctg
     9841 gttaccacgg cgtctgttcg tctggatggc gcgagcctgc acttcgcacc gctggaattc
     9901 ccggctgtcg ctgatttcga atgtacgact gcgctggttg aagctgcgaa atccattggc
     9961 gcgacgactc acgttggcgt gacagcttct tctgatacct tctacccagg tcaggaacgt
    10021 tacgatactt actctggtcg cgtagttcgt cactttaaag gttctatgga agagtggcag
    10081 gcgatgggcg taatgaacta tgaaatggaa tctgcaaccc tgctgaccat gtgtgcaagt
    10141 cagggcctgc gtgccggtat ggtagcgggg gttatcgtta accgcaccca gcaagagatc
    10201 ccgaatgctg agacgatgaa acaaaccgaa agccatgcgg tgaaaatcgt ggtggaagcg
    10261 gcgcgtcgtc tgctgaaata aaaaaacgct ccccaacttc ggggagcgat atcagttatt
    10321 gacgccgtaa acgcattatt ttgcggatta ttggcgtcag tagcgtaaag gctgccagag
    10381 tcagcaatgc cagagtgatc ggtcgggtat acagaaagtc gagtgaccca ccagaaagcg
    10441 acagcgcacg gcgaaaatta ctttccgcca ttggccccat aatcaatgcc agcactacgg
    10501 gtgatgccgg atatcctcct ttctgcatga aatagcccac cacgcctgca atcagcataa
    10561 tgccaacatc aaaaaggcta ttgttcaacg cataagtccc gaccacacaa agtgcgagga
    10621 tcatcggtgt gagaatattg ccgggaattt tcaccacatt accgatgact ttcaaagaga
    10681 gcagcccaag cactagcagc atgaagtaac agaagatcat gccgacaaac agcgtataaa
    10741 ccatatcgcc gtggtcggta aacatcagcg ggcctggttg cattccctgt aacgttaacg
    10801 cgcccagcat gatggcggtt accgcatcgc cagggatccc cagcgtcagc ataggtaaca
    10861 atgcgccacc ggtgcagcca ttcgcgcctg cctcacagga ggccacagct ttaatttcac
    10921 ccttaccaaa gttttctggt gtcttactga aacgacgagt ttcgttatag gcaacaaaag
    10981 ctgcaatatc cgcccccgca ccggggatca tgccgataaa aacccctaat cctgatgagc
    11041 gcaaaatggt taatgcaatg cggcgtagtg tttgccaggg cagtaacaga ctgccgatag
    11101 ccaccttcgc gccctgacga atttgctggt tttgctccat agagcgaaat gcttctgaag
    11161 cagcaaataa accaatcatc accgggataa atggcacatt catcagctcc acaaatccac
    11221 ctgtaaaccg tggaaaaccg cccattggat caaggcctat cgttgcgatc agcaatccgc
    11281 caaccccggc aatcagccct ttaatcagtg actcaccgga aatactggca ataatgctca
    11341 gtccgaaggt tgccagggcg aaggactccg aggcactaaa tttcagcgcc caactggcaa
    11401 gtaccggaga gagaaacatc agcacgataa tgctgagcgt tccgccacta aaggaagaga
    11461 acgtggcggc actcaatgcc agccctgctt ttccctgttt cgtcagctca taaccatcaa
    11521 tagcggtggc tgcggcggca ggtgttccgg gaattttgag caaaattgcg gtgatagaac
    11581 caccgtagac gccaccaaaa aagacgccgc aaatcatcaa caatccagaa acaggctcca
    11641 tgccgtaggt gaaaggcagc aaaatcgcca cacccatggt cgctgtcaga ccaggcaaag
    11701 cgccaatcac aatacccagc atcaccccaa aagtggcaaa cagcagcgcc tgaggattat
    11761 tgagtaatgt cgaaaaaccc tgagttaaaa gttcactttc aaacattgct ggcccctgtt
    11821 attcgaaaaa tgtgccgaca ggcaatggca cctgtaacgc ttcgacaaac agcaaccaga
    11881 ggattgccgt taacagcagc gcaatacccg gtgtgagtac ccatcgcctg tagcccatca
    11941 acgtcataat gacgatgagg aaaacaggag tagtaatgag ataaccgcag tagctcatcg
    12001 ccacaatata aaacgcggtt gccgcaattc ctgttgctgt tttcctgaat gccggttttt
    12061 cctgtgcgtt ggcgcaagag gctggttttg cctgcaaaag atcgcgtatc accagcaaga
    12121 cagcgagcac aatcagggca caggcataga aggtagggaa ggcacttgca ccgggatccg
    12181 tttcaatggc agttgctgga aaatcagcgg cattaatcat caccgcgcca gcaaccacac
    12241 aaagtaacag cgccaccacc atcgttgatg aaaggcgctt gcggtgcggt acggcgagtt
    12301 gagcaacaga ctgttttgtg ttcatcaacc gtacctcagg tttatttttt caggcccagg
    12361 cgagtcagca actcgtcaaa gtacttttcc tgttcgctga tttgggtctg gaagctggca
    12421 gcgtcaagcc atgcatagtt gagattcaac ttttgcagtg catcctggaa tgcaggctct
    12481 tcagccgtct cttttgctgc ctttgccaga acatccacca catcctgcgg cgttttttgc
    12541 gacacaatca ggccgcgcca ggtgccgatg gagagatcaa cgcctttctc ttttaacgtc
    12601 gggacgtcag gcatggtttt cattcgctca tccgccatca ctaccagtgt cttcagcttg
    12661 ccgccattca catggttgat aacttctcct gggcttacgg aaaccgcttc aatatgcccg
    12721 cctaacaggc ctgtaatggc aggggctgcg ccgtcatacg ggacatgaga aaacttcgcg
    12781 cccgttttgt cttccagtgc agctgccgcc agatgccaga ttgctccggt gcctgagtta
    12841 ccaatgcgta cttttccggg attcgctttg gcgtaagtca taaattcttc atagctattc
    12901 cacggcgcat cagaacggac ggtgatagca gccggatccg cgttcagacg ggcaatgggt
    12961 ttaaagtcgc tggttttaaa acgcaccatt ccgaggctgg gaagggtggt cagttcaacc
    13021 gtgcctaaac caattttgta accgttaggg cgggcagcgg cgatttcact caggccgata
    13081 gcaccgcctc cgccaggttt attgataacc ccgatgctga cgggtaaatg gtttttggcg
    13141 gcatcagcaa aggcacgggc aaccagatcc gtaccgcctc cggcagcgta gggaacgact
    13201 aactcgatct gtttggttgg ccaggaggca gcctgtacag gcgcaatgct gagggcagcg
    13261 cctgcaagaa gtaccattgc agccattttt ttcggtaagg taagatgttt tatcattgct
    13321 tttatatcct tatttatcag ttagttgcta tttaaattgt tccatgcgtt cataaacagt
    13381 actgcatttt catagacttg ctctggcgtc atgccggggc gatatagcgc agaaccgagg
    13441 ccaaagccgt ttgcgccgtg gctcagataa cgcgccatgg tttccggtgt gataccgcca
    13501 accggtaaca tcggaacgga ttgtggaata accgcacgcc aggctttggt aacttctggc
    13561 gtaatttgtt cagccgggaa cagcttgatg gcattagcac catgttcaat ggcagcaaag
    13621 gcttcggtgg gcgtggcgac gccaggcgca caatacatac ccagcgcacg ggcgcgacgg
    13681 atcaccgcag tatcgcaatg cggcatcacg ataatctggc ccccagcctc tttaaccgca
    13741 accacctgct ctaccgaaag cacagttccg gctccgacca ggcagtcctt aggcagggcg
    13801 tcgcgcagta aactgatact ttgcaacgcc tccggcgagt tcataggaat ctcaatcagg
    13861 cggaatccgg cggcgtacag tttttcaccg atggcgacaa tctcatccgg tttcacgccg
    13921 cgtaaaatgg cgactaaccc ggttttttgc aaaagtgtat taaatggcat gggtacattc
    13981 cctggcgttg gtggacagta acccggcagc gtgggcgatt cgccataatc catgttcagt
    14041 ggcattagga atggcgtgta tctgtctgtc gggaaaaacg tggctgaatg cttgtgaata
    14101 gcggctgttc agatttgcgc taccgattaa ggcgatcggt gttgtggcgg gtaaatcacg
    14161 tagcaaactg gatgccagac cacataattc atggcctatc agtaatccgg aaaggtaatc
    14221 cccctgcgag gacgacgtaa gattactggt cagcaggcgc gaacgggtcg aaaacagcgt
    14281 cgataataat ccggtctgat gatgctcttg tgctgctgtc agcccgtggg tgaaggcatc
    14341 ccagtgcggt tcatctccag gatgcatggt ggcaccgaga atcgaatggc gggataaaac
    14401 atcaaataac tcgcccgtca taaaggttcg gaaatcggta atggtgttgt tttcaactac
    14461 tgcccatttc gcatgtgtgc cgggtaagcc tatcagcact ggcataccct gatggatcgc
    14521 gttttgcagg gccggttcca tcgagatagc accgaagatt tgtgtctctt cgccacgcat
    14581 cacttcgggc atttcacctt ctttaatcac gccaggaatg atgtgcagca ttgtctgctg
    14641 ctggtggata acaggcgtca actgcttcgc cagaccctcg agagtgaccg ggcaaggtaa
    14701 ataagctgct gattgccacc cttgcgcact gccgagcatg ccgcaagcca gtaccgggca
    14761 tggggtttgt gccagccagt cgccgcagac acgtaaaaac gtatcgtgga aagcgtcctc
    14821 acgtgttgct gcctgggagg gtaatttcat gatcccccag gggaattcac gtttttcttg
    14881 tggatttggt gattcgccaa aacgccatgc ccgaagcgat gacgttcccc agtccagggc
    14941 aataaatgag ggtgctcccg gcattagtcg tttcctttaa tcatggttta tttatctttg
    15001 cggacgtgct taccccagcc cagttcagcg gatatctctt ctgcacagga tttgacgtaa
    15061 ggtgccagtt cttccagacg cgccagcgac atatacgtgg tggtactggc gacagaaata
    15121 gccgccacaa tttcatcccg ggcgttgtaa accggcgctg caacgcaacg gatagtcggc
    15181 tcgttctctt ccagatcaaa agcaaaacca ctggcggcat aacgacgcat gttctgaata
    15241 aatgcgccca gttttgtttc atcaccacag gtcttaaaca gcgtgcgcca ctcttcttct
    15301 gttcggttga ggatcaatgc cttgccgatg ccggtgattg ccagtggcat gcgatggcct
    15361 gggcgtgagc gcatttccag cccgcgctga ctgttgatct tctccagata cagcacttcg
    15421 tcgccttcaa cgatccccag atgcacggtg tccagggtgt aacgggcaag gcgttgcaaa
    15481 acgggctggg caatttcgta cagcgaggtg ctttccagtg ctctggcacc aaattcgatc
    15541 agtttggcac ccagttggta gccctgcgtt gggacatggc gcaggtagcg ttgttccacc
    15601 agaacggtga ctaaacgatg ggttgtgctc cgggtcgtgc cggtgaactc gccgatactt
    15661 tttaggtcat ggcagccgtt ataaacagca ttaagaaccg ccagtccacg taataacgtt
    15721 tgtgcgcctg cggtggttga agaggtttct gccatacgtt tctcctgatg atattgctga
    15781 agtgttccac ataatgacgt ggggaaaagg tgatatatcc cacaatataa actccatctc
    15841 atattgtggg ataaaataat gaaaattaac gatcgttttg cgtagcgcct cgcaggaaac
    15901 ttctttcaaa ctggacgttt gtacagcaca attctatttt gtgcgggtaa gttgttgcgt
    15961 caggaggcgt tgtggatttc tcaatcatgg tttacgcagt tattgcattg gtgggtgtgg
    16021 caattggctg gctgtttgcc agttatcaac atgcgcagca aaaagccgag caattagctg
    16081 aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca acaaattacc caaagcgagc
    16141 actggcgcgc agagtgtgaa ttgctcaata acgaagtgcg cagcctgcaa agtattaaca
    16201 cctcgctgga ggccgatctg cgtgaagtga ccacgcggat ggaagccgca cagcaacacg
    16261 ctgacgataa aattcgccag atgatcaaca gcgaacagcg cctcagtgag caatttgaaa
    16321 accttgccaa ccgtattttt gaacacagta atcgtcgggt tgatgagcaa aatcgccaga
    16381 gtctgaacag tcttttgtcg ccgttgcgtg aacaactgga tggtttccgc cgtcaggtcc
    16441 aggacagctt tggtaaagaa gcccaagaac gtcataccct gactcacgaa atccgcaacc
    16501 tccagcaact caacgcccag atggcccagg aagcgatcaa cctgacgcgt gcgctgaaag
    16561 gcgacaataa aacccagggc aactggggcg aggtagtatt gacgcgggtg ctggaggctt
    16621 ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag catcgaaaat gacgcccgct
    16681 cgcggatgca gccggatgtc atcgtgcggt tgccgcaggg aaaagatgtg gtgatcgacg
    16741 ccaaaatgac gctggtcgcc tatgaacgct atttcaacgc cgaagatgac tacacccgcg
    16801 aaagcgcgct acaggaacat atcgcgtcgg tacgtaacca tatccgtttg ctgggacgca
    16861 aagattatca acagctgccg gggctgcgaa ctctggatta cgtgctgatg tttattcccg
    16921 ttgaacccgc ttttttactg gcgcttgacc gtcagccgga gctgatcacc gaagcgttga
    16981 aaaacaatat catgctggtt agcccgacta cgctgctggt ggcgctgcgc actatcgcca
    17041 acctgtggcg ttatgagcat caaagtcgta acgcccagaa aatcgccgat cgcgccagca
    17101 agctgtacga caaaatgcgc ttgtttgtcg atgacatgtc cgcgatcggt caaagtctcg
    17161 acaaagcgca ggataactat cgccaggcaa tgaaaaaact ctcttcaggt cgcggaaatg
    17221 tgctggcgca ggcagaagcg tttcgcggtt taggggtaga aattaaacgc gagattaatc
    17281 cggatttggc tgaacaggcg gtgagccagg atgaagagta tcgacttcgg tcggttccgg
    17341 agcagccgaa tgatgaagct tatcaacgcg atgatgaata taatcagcag tcgcgctagc
    17401 ccattggggg tagttaagcc gggtagaaat ctagggcatc gacgcccaat ctgttacact
    17461 tctggaacaa ttttttgatg agcaggcatt gagatggtgg ataagtcaca agaaacgacg
    17521 cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc ccacgttttc
    17581 cattccgtgg catcaaaata cgatgtcatg aatgatttga tgtcattcgg tattcatcgt
    17641 ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat
    17701 ctggctggtg gcaccggcga cctgacagcg aaattctctc gcctggtcgg agaaactggc
    17761 aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa tgggccgcga gaagctgcgt
    17821 aatatcggtg tgattggcaa cgttgagtat gttcaggcga acgctgaggc gctgccgttc
    17881 ccggataaca cctttgattg catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa
    17941 gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct gctggtactt
    18001 gagttctcga agccaattat cgagccgctg agcaaagcct atgatgcata ctccttccat
    18061 gtgctgccgc gtattggctc actggtcgcg aacgacgccg acagctaccg ttatctggca
    18121 gaatccatcc gtatgcatcc cgatcaggat accctgaaaa ccatgatgca ggatgccgga
    18181 ttcgaaagtg tcgactacta caatctgacg gcaggggttg tggcgctgca tcgtggttat
    18241 aagttctgac aggagaccgg aaatgccttt taaaccttta gtgacggcag gaattgaaag
    18301 tctgctcaac accttcctgt atcgctcacc cgcgctgaaa acggcccgct cgcgtctgtt
    18361 gggcaaagtg ctgcgcgtgg aggtaaaagg cttttcgacg tcattgattc tggtgttcag
    18421 cgaacgccag gtcgatgtac tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc
    18481 ttacgccagc gtgttgccga aacttcgcga tcgccagcag cttgccgcac tgattcgcag
    18541 tggtgagctg gaagtgcagg gtgatattca ggtggtgcaa aacttcgttg cgctggtaga
    18601 tctggcagag ttcgaccctg cggaactgct ggccccttat accggtgata tcgccgctga
    18661 aggtatcagc aaagccctgc gcggaggcgc aaagttcctg catcacggca ttaagcgcca
    18721 gcaatgttat gtggcggaag ccattactga agagtggcgt atggcacccg gtccgcttga
    18781 agtggcctgg tttgcggaag agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa
    18841 acggctggaa aaactggagg ctaaatgacg ccaggtgaag tacggcgcct atatttcatc
    18901 attcgcactt ttttaagcta cggacttgat gaactgatcc ccaaaatgcg tatcaccctg
    18961 ccgctacggc tatggcgata ctcattattc tggatgccaa atcggcataa agacaaacct
    19021 ttaggtgagc gactacgact ggccctgcaa gaactgggac cggtatggat caagttcggg
    19081 cagatgttat caacccgccg cgatcttttt ccgccgcata ttgccgatca gctggcgtta
    19141 ttgcaggaca aagtcgctcc gtttgatggc aagctggcga agcagcagat tgaagctgca
    19201 atgggcggct tgccggtaga agcgtggttt gacgattttg aaatcaagcc gctggcttct
    19261 gcttctatcg cccaggttca taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt
    19321 aaagttatcc gcccggatat tttgccggtc attaaagcgg atctgaaact tatttaccgt
    19381 ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg
    19441 gtgcgcgagt acgaaaagac cttgattgat gaactgaatt tgctgcggga atctgccaac
    19501 gccattcagc ttcgacgcaa ttttgaagac agcccgatgc tctacatccc ggaagtttac
    19561 cctgactatt gtagtgaagg gatgatggtg atggagcgta tttacggcat tccggtgtct
    19621 gatgttgcga cgctggagaa aaacggcacc aacatgaaat tgctggcgga acgcggcgtg
    19681 caggtgttct tcactcaggt ctttcgcgac agctttttcc atgctgatat gcaccctggc
    19741 aacatcttcg taagctatga acacccggaa aacccgaaat atatcggcat tgattgcggg
    19801 attgttggct cgctaaacaa agaagataaa cgctatctgg cggaaaactt tatcgccttc
    19861 tttaatcgcg actatcgcaa agtggcagag ctacacgtcg attctggttg ggtgccacca
    19921 gataccaacg ttgaagagtt cgaatttgcc attcgtacgg tctgtgaacc tatctttgag
    19981 aaaccgctgg ccgaaatttc gtttggacat gtactgttaa atctgtttaa tacggcgcgt
    20041 cgcttcaata tggaagtgca gccgcaactg gtgttactcc agaaaaccct gctctacgtc
    20101 gaaggggtag gacgccagct ttatccgcaa ctcgatttat ggaaaacggc gaagcctttc
    20161 ctggagtcgt ggattaaaga tcaggtcggt attcctgcgc tggtgagagc atttaaagaa
    20221 aaagcgccgt tctgggtcga aaaaatgcca gaactgcccg aactggttta cgacagtttg
    20281 cgccagggca agtatttaca gcatagtgtt gataagattg cccgcgagct tcagtcaaat
    20341 catgtacgtc agggacaatc gcgttatttt ctcggaattg gcgctacgtt agtattaagt
    20401 ggcacattct tgttggtcag ccgacctgaa tgggggttga tgcccggctg gttaatggca
    20461 ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt tcatcgctca
    20521 aggcgggccg tgtaacgtat aatgcggctt tgtttaatca tcatctacca cagaggaaca
    20581 tgtatgggtg gtatcagtat ttggcagtta ttgattattg ccgtcatcgt tgtactgctt
    20641 tttggcacca aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt
    20701 aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa ccagccagga tgctgatttt
    20761 actgcgaaaa ctatcgccga taagcaggcg gatacgaatc aggaacaggc taaaacagaa
    20821 gacgcgaagc gccacgataa agagcaggtg taatctgtgt ttgatatcgg ttttagcgaa
    20881 ctgctattgg tgttcatcat cggcctcgtc gttctggggc cgcaacgact gcctgtggcg
    20941 gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac
    21001 gaactgaccc aggagttaaa actccaggag tttcaggaca gtctgaaaaa ggttgaaaag
    21061 gcgagcctca ctaacctgac gcccgaactg aaagcgtcga tggatgaatt acgccaggct
    21121 gcggagtcga tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg
    21181 cacaccatcc ataacccggt ggtgaaagac aatgaaactg tgcatgaagg cgtaacgcct
    21241 gctgctgcac aaacgcaggc cagttcgccg gaacagaagc cagaaaccac gccagagccg
    21301 gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt
    21361 gataaaccgt aaacatgtct gtagaagata ctcaaccgct tatcacgcat ctgattgagc
    21421 tgcgtaagcg tctgctgaac tgcattatct cggtgatcgt gatattcctg tgtctggtct
    21481 atttcgccaa tgacatctat cacctggtat ccgcgccact gatcaagcag ttgccgcaag
    21541 gttcaacgat gatcgccacc gacgtggcct cgccgttctt tacgccgatc aagctgacct
    21601 ttatggtgtc gctgattctg tcagcgccgg tgattctcta tcaggtgtgg gcgtttatcg
    21661 ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc
    21721 tgctgtttta tatcggcatg gcgttcgcct actttgtggt ctttccgctg gcatttggct
    21781 tccttgccaa taccgcgccg gaaggggtac aggtatccac cgacattgcg agctatttaa
    21841 gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt tgaagtgccg gtggcaattg
    21901 tgttgctgtg ctggatgggg attacctcgc cagaagactt acgcaaaaaa cgcccgtatg
    21961 tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa
    22021 cgctgttggc gatccctatg tactgcctgt ttgaaatcgg tgtcttcttc tcacgctttt
    22081 acgttggtaa agggcgaaac cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa
    22141 ctgaagaata aattcaaccg cccgtcaggg cggttgtcat atggagtaca ggatgtttga
    22201 tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg
    22261 cgcttttgac gcgggagtta atgggctact catcaccggt accaatctgc gtgaaagcca
    22321 gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc
    22381 tcacgacagc agccagtggc aagctgtgac tgaagaagcg attattgagc tggccgcgca
    22441 gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc
    22501 ggaagagcag gaactcgctt ttgttgccca gctacgcatt gccgcagaat taaacatgcc
    22561 ggtatttatg cactgtcgcg atgcccacga gcggtttatg acattgctgg agccgtggct
    22621 ggataaactg cctggtgcgg ttcttcattg ctttaccggc acacgcgaag agatgcaggc
    22681 gtgcgtggcg cgtggaattt atatcggcat taccggttgg gtttgcgatg aacgacgcgg
    22741 gctggagctg cgggaattgt tgccgttgat tccggcggag aaattgctga tcgaaactga
    22801 tgcgccgtat ctgctccctc gcgatctcac gccaaagcca tcatcccggc gcaacgagcc
    22861 agcccatctg ccccatattt tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg
    22921 gctggctgcc accacggatg ctaatgtcaa aacactgttt gggattgcgt tttagagttt
    22981 gcggaactcg gtgttcttca cactgtgctt aatctcttta ttaataagat taagcaatag
    23041 catggagcga gcctcgccgt ctggctcggt gaaaatggcc tgaaaacctt cgaatgcgcc
    23101 ttcagtaata atcactttat caccaggata cggcgtcgat ggatcgacaa tatctttcgg
    23161 tttatacact gataactgat gaatcaccgc cgatgggact atcgctggtg acgcgccaaa
    23221 gcgcacgaag tggctgacac cgcgggtcgc gttgatagtc gtggtatgaa tcacttccgg
    23281 atcgaactcc acaaacaagt agttggggaa caatggctca ctgactgcag tacgtttgcc
    23341 acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa ttcacagcct gtctttcgag
    23401 gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac agtaaatacc aggattgcat
    23461 aatgactctt atccgtttaa tcggggcgca aggatagcaa aagctttacg ctaagttaat
    23521 tatattcccc ggtttgcgtt ataccgtcag agttcacgct aatttaacaa atttacagca
    23581 tcgcaaagat gaacgccgta taatgggcgc agattaagag gctacaatgg acgccatgaa
    23641 atataacgat ttacgcgact tcttgacgct gcttgaacag cagggtgagc taaaacgtat
    23701 cacgctcccg gtggatccgc acctggaaat cactgaaatt gctgaccgca ctttgcgtgc
    23761 cggtgggcct gcgctgttgt tcgaaaaccc taaaggctac tcaatgccgg tgctgtgcaa
    23821 cctgttcggt acgccaaagc gcgtggcgat gggcatgggg caggaagatg tttcggcgct
    23881 gcgtgaagtt ggtaaattat tggcgtttct gaaagagccg gagccgccaa aaggtttccg
    23941 tgacctgttt gataaactgc cgcagtttaa gcaagtattg aacatgccga caaagcgact
    24001 gcgtggtgca ccctgccaac aaaaaatcgt ctctggcgat gacgtcgatc tcaatcgcat
    24061 tcccattatg acctgctggc cggaagatgc cgcgccgctg attacctggg ggctgaccgt
    24121 gacgcgcggc ccgcataaag agcggcagaa tctgggcatt tatcgccagc agctgattgg
    24181 taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc ggcgcgttgg attatcagga
    24241 gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct gtggcgctgg gtgccgatcc
    24301 tgccacgatt ctcggtgcag tcacccccgt tccggatacg ctttcagagt atgcgtttgc
    24361 cggattgctg cgcggcacca agaccgaagt ggtgaagtgt atctccaatg accttgaagt
    24421 gcccgccagt gcggagattg tgctggaagg gtatatcgaa caaggcgaaa ctgcgccgga
    24481 agggccgtat ggcgaccaca ccggttacta taacgaagtc gatagtttcc cggtatttac
    24541 cgtgacgcat attacccagc gtgaagatgc gatttatcat tccacctata ccgggcgtcc
    24601 gccagatgaa cctgcggtac tgggtgtagc actgaacgaa gtgttcgtgc cgattctgca
    24661 aaaacagttc ccggaaattg tcgattttta cctgccgccg gaaggctgtt cttatcgtct
    24721 ggcggtagtg acgatcaaaa aacagtacgc cggacacgcg aagcgcgtca tgatgggcgt
    24781 ctggtcgttc ttacgccagt ttatgtacac taaatttgtg atcgtttgcg atgatgacgt
    24841 caacgcacgc gactggaacg atgtgatttg ggcgattacc acccgtatgg acccggcgag
    24901 ggatactgtt ctggtagaaa atacgcctat tgattatctg gattttgcct cgcctgtctc
    24961 cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa tggccgggtg aaacccagcg
    25021 tgaatgggga cgtcccatca aaaaagatcc agatgttgtc gcgcatattg acgccatttg
    25081 ggatgaactg gctattttta acaacggtaa aagcgcctga tgcgcgtttg ttttgcccta
    25141 tttatcgatc cgacagagaa agcgcatgac aaccttaagc tgtaaagtga cctcggtaga
    25201 agctatcacg gataccgtat atcgtgtccg catcgtgcca gacgcggcct tttcttttcg
    25261 tgctggtcag tatttgatgg tagtgatgga tgagcgcgac aaacgtccgt tctcaatggc
    25321 ttcgacgcca gatgaaaaag ggtttatcga gctgcatatt ggcgcttctg aaatcaacct
    25381 ttacgcgaaa gcagtcatgg accgcatcct caaagatcat caaatcgtgg tcgatattcc
    25441 ccacggagaa gcgtggctgc gcgatgatga agagcgtccg atgattttga ttgcgggcgg
    25501 caccgggttc tcttatgccc gctcgatttt gctgacagcg ttggcgcgta acccaaaccg
    25561 tgatatcacc atttactggg gcgggcgtga agagcagcat ctgtatgatc tctgcgagct
    25621 tgaggcgctt tcgttgaagc atcctggtct acaagtggtg ccggtggttg aacaaccgga
    25681 agcgggctgg cgtgggcgta ctggcaccgt gttaacggcg gtattgcagg atcatggtac
    25741 gctggcagag catgatatct atattgccgg acgttttgag atggcgaaaa ttgcccgtga
    25801 cctgttttgc agcgagcgta atgcgcggga agatcgcctg tttggcgatg cgtttgcatt
    25861 tatctgagat ataaaaaacc cgcccctgac aggcgggaag aacggcaact aaaactttat
    25921 acagtggcat tactctacgt tgcatccggc aaacagtgcc cgatgcaacg ctgccgcgtc
    25981 ttatcgggcc tacaaaagtt ctgaaccgtg ggccggataa ggcgttcacg ccgtatccgg
    26041 catacagtgc ccgatgcgac gctgccgcgt cttatcgggc ctacaaaagt tctgaaccgt
    26101 aggccggata aggcgttcac gccgcatccg gcaagttgtt aaacccgctc aaacaccgtt
    26161 gcaatgccct gacccagacc gatacacatc gtcgccagac caaactggac gtctttgtgt
    26221 tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg aacaacccag cggatgaccc
    26281 agcgcgatcg cgccaccgtt gaggttgatc ttctcgtcaa tctgctccat tagcccgaga
    26341 tctttaatac acggcaggat ctgcgcggca aaggcttcgt tcatctcgaa tacgccgata
    26401 tcgctggcag aaagccccgc ttttttcagc gccagcttcg aggccggaac cggaccataa
    26461 cccataatag atgggtcaca accaacgacc gccatcgaac gcacgcaggc gcgcggctta
    26521 agacctaagt cacgggcgcg gctttcactc atcaccagca tggcagctgc gccatcggaa
    26581 agcgcagaag atgtacccgc cgttaccgta ccgctgacgg gatcaaacgc cggacgcagt
    26641 gtggcgaggg cttccacggt ggtttccggg cgaatcactt cgtcgtaatt aaactgcttc
    26701 aatacgccgt cggcatcgtg accaccggtc gggatgattt catttttaaa tgcgcctgac
    26761 tgcgtggcgg cccacgcgcg ggcgtgtgac cgcgcggcaa aggcatcctg catttcacgg
    26821 ctgataccgt gcatacgcgc cagcatttct gccgttaagc ccatcatgcc cgccgctttg
    26881 gcgacattgc ggctcaggcc ggggtgaaaa tcgacaccgt ggctcattgg cacatggccc
    26941 atatgctcca cgccgccaac cagacatgcc tgcgcatcgc cagtcataat catccgtgct
    27001 gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat tgacggtcac cgccgggaca
    27061 gagtgcggca cttctgccaa cagcgccgcg ttacgggcga tgttaaaacc ctgctccagt
    27121 gtttgctgca cacaacccca gtaaatatcg tcgagggccg ctgcttccag cgccgggtta
    27181 cgcgccagca ggctacgcat taaatgagcg gagagatctt ccgcacgcac gtgacgaaaa
    27241 gcaccgccct tcgaacgacc catcggggtg cgaattgcat cgacaatgac aacctgttcc
    27301 attgtgactc cttaagccgt tttcaggtcg ccaaccggac gggctggctc aaccggggga
    27361 tagtacggtt cgttatgacg tgctttatta cgcagacctt ccggtacttc atacagcggg
    27421 ccgaggtgct gatattgctg tgccatatcg aggtattttg cgctgccgag ggtgtccagc
    27481 cagcggaacg cgccgccgtg gaacggaggg aagcccaggc cgtagaccag cgccatatcc
    27541 gcttctgccg gagtggcgat aatgccctct tccagacagc ggaccacttc gttgaccatc
    27601 gggatcatca tgcgggcgat aatctcttct tcgctgaaat cgcgctttgc gtggctgacc
    27661 tctgccagca ggtcgtcaac gacggcatct tcttctttct tcggcttacc tttgctgtct
    27721 tctttataac gccagaaacc gaggccgttc ttctgaccaa agcggttggc attaaacagc
    27781 gcgtcgatgg catcgcgata atctttctgc atccgctgcg ggaagcctgc tgccatgaca
    27841 gcctgggcgt gatgcgcggt gtcaatgccc acaacatcca gcagatatgc cggacccatc
    27901 ggccagccaa actgtttttc catcactttg tcgatcttgc ggaaatccgc gccgtcgcgc
    27961 agcaactggc tgaaaccggc gaaatagggg aacagtacac ggttgacgaa gaagccgggg
    28021 cagtcgttaa ccacaatcgg cgttttgccc atcttgctcg cccaggcgac gactttcgcg
    28081 atggtttcgt cggagctttt ctcgccgcga ataatttcta ctaacggcat tcggtggacc
    28141 gggttaaaga agtgcatccc gcagaagttt tccggacgtt ccagcgcgtt ggccagttcg
    28201 ctgataggaa tggttgaagt gttagacgcc agtacggtat ccgggcgtac tttctgctcg
    28261 gtttctgcca gcactgcttt tttcactttc gggttttcaa caaccgcttc taccacaaca
    28321 tccacgcggt caaacccggc gtagttgagc gttgggtgaa ttgtggagat cacgccagcc
    28381 agtttcagac catcgatctt gccgcgctca agctgcttgt tcagcagttt cgcggcttcg
    28441 gtcatgccga gggttaacga cttgtcgttg atatctttca tgacaaccgg cacgcctttc
    28501 cacgcagact ggtaggcgat gccgccgccc ataatgcctg cgcccagcac agcggcctgt
    28561 ttcggggttt caacatcttt ggtgagtttc ttcgctttac cttttacata ttgatcgtta
    28621 aggaaaatgc cgaccagtgc gcgggcttcg ttggtatgcg ccagcgggac aaaacttttg
    28681 ttttccaggt ttaaggcttc ttcacgacca aaacgggccg cagcttcaat ggtttttact
    28741 gcggtgatgg gggccggata atgtttcccc gccgtttgcg cgaccatccc tttagcgatg
    28801 gtgaagctca tggcggcttc aatcttgctc agttttagcg gttccagctt cggctgacgt
    28861 tttgctttcc agtcgaggtc gccgttaatg gcctggcgta aaatcgctat cgccccttca
    28921 accagttttt ctgctttgac tacgccatcc accagaccga ttttcagcgc ctgatccgcg
    28981 ccgacatctt taccggcggc aatgatttcc agcgcactgt cagcgcccag catacgtggc
    29041 atacgtacag aaccgccaaa gcccggcatg atgcccaatt tggtttccgg cagaccgatg
    29101 cgcagatccg gcgtcgccag acgataatcg gtcgccagca cgcattcgca gccaccgccc
    29161 agcgcatagc cgttgacagc agcaatggtc ggcaccggca gatcttccag gcgattaaac
    29221 acgctattgg caaagtgcag ccactgactt aactgttctt caggaacgag gaacagggac
    29281 aaaaattcgg tgatatcagc accgacgata aaggctgctt tgttcgaacg tagcagcagt
    29341 ccttttagat ctgattgctg ttccagcacg ccgatggcct cgccgaggct ggcgacggtc
    29401 gcagtgtcga gtttattaac tgaacctggg gcatcaaata ccagttcggc aatgccatct
    29461 tccagccagt caaggtacag ggtgtcgcct ttgtaaagca tgtcagtctc ctgaatccgc
    29521 aaggtgatct ggtcgtacca gatgcatcga agtgtgtatt ttatgttaaa aatatgcaaa
    29581 taaaatatta aggaaatgcc aatctgatca cgctcggcag aaatcacgct ctggatgaac
    29641 gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga agggtaaaaa atggaatcac
    29701 tggcctcgct ctataaaaat catatagcta ccttacagga acggactcgc gatgcgctgg
    29761 cgcgcttcaa gctggatgcg ttacttattc actccggcga actgttcaac gtttttctcg
    29821 acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa agcgtgggtg ccggtaactc
    29881 aggtgccaaa ctgctggctg ctggtggatg gcgtgaataa gccgaaactg tggttttatc
    29941 tgccggttga ttactggcac aacgtcgaac cgctgccgaa ctccttctgg actgaagatg
    30001 tagaagtgat cgcgctgccg aaagccgatg gcattggtag tctgttgcct gctgcgcgtg
    30061 gcaatatcgg ttatatcggt ccggtgccag aacgtgcgct gcaactgggt attgaggcca
    30121 gcaatatcaa cccgaaaggg gtgatcgact acctgcatta ctatcgctcc ttcaaaaccg
    30181 agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc ggtcaacggt catcgcgcgg
    30241 cagaagaagc gttccgttct ggcatgagcg agttcgatat caacattgcc tatctgaccg
    30301 cgaccggtca tcgtgatacc gacgtacctt atagcaacat tgtggcgctt aacgaacacg
    30361 ctgcggtgtt gcattacacc aaactggacc accaggcacc ggaagagatg cgcagcttcc
    30421 tgctggatgc cggggccgaa tataacggct atgcggctga tctgacccgt acctggtcgg
    30481 caaaaagcga caacgactac gcacagctgg tgaaagacgt aaatgatgag caactggcgc
    30541 tgatcgccac catgaaagca ggcgtcagct atgtggatta ccatatccag ttccatcagc
    30601 gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga tatgagtgaa gaagcgatgg
    30661 tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat cggccatccg ctgggcctgc
    30721 aggtgcatga cgtagccggt tttatgcagg atgatagcgg tacgcacctc gcggcaccgg
    30781 caaaatatcc gtacctgcgc tgcacccgta ttctccagcc gggcatggtg ttaaccatcg
    30841 aaccgggtat ctacttcatc gaatcgcttc tggcaccgtg gcgtgaaggg cagttcagca
    30901 aacacttcaa ctggcagaaa attgaagcac tgaaaccgtt cggcggcatt cgtatcgaag
    30961 acaacgtggt gattcacgaa aacaacgtgg aaaacatgac ccgggatctg aaactggcgt
    31021 gatggaaagc tggttaattc ctgcggcacc ggtcacggtc gttgaagaga tcaaaaagag
    31081 ccgcttcatt acgctgttgg cgcataccga tggcgttgag gcggcaaaag cgtttgttga
    31141 atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc gtggcgtggg tggcgggtgc
    31201 gccggatgat tctcaacagc tgggtttctc tgacgacggg gagccggcgg gaacggcagg
    31261 taaaccgatg ctcgcccaac taatgggcag cggcgtcggg gaaattaccg ctgtggtggt
    31321 gcgctactac ggcggcatat tgcttggcac cggtgggtta gtgaaagcgt atggcggcgg
    31381 cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag acaccattaa ccgaatatac
    31441 tttgcaatgt gaatatagtc agttaaccgg cattgaagcg ttgctggggc agtgtgacgg
    31501 caaaattatt aacagtgatt atcaggcatt cgttctgctg cgggtggcgc ttccggcggc
    31561 gaaagtggct gaattttccg caaagctggc ggattttagc cgtggttcat tgcaattgtt
    31621 agcgattgaa gaataatccc cacttcgttt tgcagaacta aggaagcggc agagatgcat
    31681 tttcgcgcca ttacccgaat cgttggacta ctggtcatct tattttcagg gaccatgatt
    31741 atccctgggc tggtagcact catctaccgg gatggagcgg gccgcgcttt tacccagacc
    31801 ttttttgtcg ccctcgccat tggctctatg ctttggtggc cgaaccgcaa agagaagggc
    31861 gaactgaaat cccgtgaggg gtttctgata gtggtgctgt tctggaccgt gctgggtagc
    31921 gtcggtgcgc tcccttttat cttctcggaa agcccgaacc tcacgattac cgatgcgttt
    31981 tttgaatctt tctctggcct gaccaccacg ggggccacta cgctggtggg gctggattcg
    32041 ctccctcatg ccatcctctt ttatcgccag atgctgcaat ggtttggcgg tatggggatc
    32101 atcgtgctgg cggttgcgat actgcctatc ctcggcgtgg gtgggatgca gctctatcgc
    32161 gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc cgcgaattgc ggaaacggcg
    32221 aaaaccctgt ggttgattta tgtcttgctg accgtcgcct gtgcgctggc gttgtggttt
    32281 gctggaatgg atgcctttga tgccatcggc catagctttg cgaccatcgc tattggcggc
    32341 ttctcgacac atgatgccag tatcggttat ttcgacagcc cgactattaa cactatcatt
    32401 gctatcttcc tgctgatctc tggctgtaac tacggtctgc acttttcact gttaagtggg
    32461 cgtagtctga aggtttattg gcgcgatccg gaatttcgca tgtttatcgg cgtacagttt
    32521 tcactggtgg ttatttgtac actcgtactg tggtttcata atgtctacag ttcggcgctg
    32581 atgacaatta accaggcgtt tttccaggtg gtgtcgatgg cgacaaccgc cgggtttaca
    32641 actgacagca ttgcccgctg gccgctcttt ttgccggtac tgcttttatg ttcagctttt
    32701 atcggcggtt gtgccgggtc aacgggcggt ggcctgaaag tgatccgcat cctgctgctg
    32761 tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc cgaacgccgt ctatagcatt
    32821 aagctgggga atcgcgcact gccggaacgt atcctcgaag ccgtttgggg atttttctcc
    32881 gcctatgcat tggtgtttat tgtcagtatg ctggcgatta tcgccacggg cgtggatgac
    32941 ttttctgcct ttgcctcggt tgttgcaaca ttgaataacc tggggccagg gcttggcgtg
    33001 gttgctgata actttaccag tatgaacccg gtggctaaat ggatcctgat tgccaacatg
    33061 ctgtttggtc gtctcgaggt ctttacattg ctggtgctct ttaccccgac tttctggcgt
    33121 gaatgatgga gtaatacgtg aaaacattaa ttcttttctc aacaagggac ggacaaacgc
    33181 gcgagattgc ctcctacctg gcttcggaac tgaaagaact ggggatccag gcggatgtcg
    33241 ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta tgaccgtgtg gttattggcg
    33301 cttctattcg ctatggtcac taccattctg cgttccagga atttgtcaaa aagcatgcga
    33361 cgcggttgaa ttcgatgccg agtgcctttt actccgtaaa tctggtggcg cgtaaaccgg
    33421 agaagcgtac tccacagacc aacagctacg cgcggaagtt tctgatgaac tcgcaatggc
    33481 gtcccgatcg ctgtgccgtc attgccgggg cgctgcgtta cccacgttat cgctggtacg
    33541 accgttttat gatcaagctg attatgaaga tgtcaggcgg tgaaacggat acgcgcaaag
    33601 aagttgtcta taccgattgg gagcaggtgg cgaatttcgc ccgagaaatc gcccatttaa
    33661 ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa tgcgcggtca gaaaattatt
    33721 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
    33781 caacggcaaa cacgccgccg ggtcagcggg attctcctga gaattccggt agagaaaagc
    33841 aaaaataaat gcttgactct
//
LOCUS       DAZKHK010000045        33190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKHK010000045 DAZKHK010000000
VERSION     DAZKHK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.045"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            254..367
                     /gene="yhiY"
                     /locus_tag="ACHOS5_002146"
     CDS             254..367
                     /gene="yhiY"
                     /locus_tag="ACHOS5_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP9776962.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
     gene            430..1497
                     /gene="yhiI"
                     /locus_tag="ACHOS5_002147"
     CDS             430..1497
                     /gene="yhiI"
                     /locus_tag="ACHOS5_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9776963.1"
                     /translation="MDKSKRHLAWWGVGALAVAAVVAWLLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPEDLVVRLPQ"
     gene            1494..4229
                     /gene="rbbA"
                     /locus_tag="ACHOS5_002148"
     CDS             1494..4229
                     /gene="rbbA"
                     /locus_tag="ACHOS5_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP9776964.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKTEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEEMRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4229..5353
                     /gene="yhhJ"
                     /locus_tag="ACHOS5_002149"
     CDS             4229..5353
                     /gene="yhhJ"
                     /locus_tag="ACHOS5_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9776965.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            5426..5698
                     /locus_tag="ACHOS5_002150"
     CDS             5426..5698
                     /locus_tag="ACHOS5_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9776966.1"
                     /translation="MRTQVQALRKKKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIK
                     EGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5695..6054
                     /locus_tag="ACHOS5_002151"
     CDS             5695..6054
                     /locus_tag="ACHOS5_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9776967.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6104..6964)
                     /locus_tag="ACHOS5_002152"
     CDS             complement(6104..6964)
                     /locus_tag="ACHOS5_002152"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFP9776968.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLIRKAS"
     gene            complement(6996..7265)
                     /locus_tag="ACHOS5_002153"
     CDS             complement(6996..7265)
                     /locus_tag="ACHOS5_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP9776969.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            complement(7255..8763)
                     /locus_tag="ACHOS5_002154"
     CDS             complement(7255..8763)
                     /locus_tag="ACHOS5_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9776970.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQSVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAVSLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWESWYQLKLQQEGVMPLHHREEHYVD"
     gene            complement(8756..10114)
                     /locus_tag="ACHOS5_002155"
     CDS             complement(8756..10114)
                     /locus_tag="ACHOS5_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9776971.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10191..10472)
                     /locus_tag="ACHOS5_002156"
     CDS             complement(10191..10472)
                     /locus_tag="ACHOS5_002156"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9776972.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(10469..10942)
                     /locus_tag="ACHOS5_002157"
     CDS             complement(10469..10942)
                     /locus_tag="ACHOS5_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9776973.1"
                     /translation="MQGIQFQENFIQRLPAGLSVEQIIHQLAQPLVTAELVVPDFADH
                     VLEREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVVFADMGGDSDPVPVR
                     VIFLLALGESNKQLNALGWIMEMIQDTPFMRALLTMETTEIHTAILNKMKERGEI"
     gene            complement(10967..11713)
                     /locus_tag="ACHOS5_002158"
     CDS             complement(10967..11713)
                     /locus_tag="ACHOS5_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HFP9776974.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(11912..12313)
                     /gene="nikR"
                     /locus_tag="ACHOS5_002159"
     CDS             complement(11912..12313)
                     /gene="nikR"
                     /locus_tag="ACHOS5_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP9776975.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(12319..13125)
                     /gene="nikE"
                     /locus_tag="ACHOS5_002160"
     CDS             complement(12319..13125)
                     /gene="nikE"
                     /locus_tag="ACHOS5_002160"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP9776976.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLMLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(13122..13886)
                     /gene="nikD"
                     /locus_tag="ACHOS5_002161"
     CDS             complement(13122..13886)
                     /gene="nikD"
                     /locus_tag="ACHOS5_002161"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP9776977.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(13886..14719)
                     /gene="nikC"
                     /locus_tag="ACHOS5_002162"
     CDS             complement(13886..14719)
                     /gene="nikC"
                     /locus_tag="ACHOS5_002162"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP9776978.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFIDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14716..15660)
                     /gene="nikB"
                     /locus_tag="ACHOS5_002163"
     CDS             complement(14716..15660)
                     /gene="nikB"
                     /locus_tag="ACHOS5_002163"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP9776979.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(15660..17234)
                     /gene="nikA"
                     /locus_tag="ACHOS5_002164"
     CDS             complement(15660..17234)
                     /gene="nikA"
                     /locus_tag="ACHOS5_002164"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9776980.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(17345..17932)
                     /gene="acpT"
                     /locus_tag="ACHOS5_002165"
     CDS             complement(17345..17932)
                     /gene="acpT"
                     /locus_tag="ACHOS5_002165"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP9776981.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYQSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(17987..19036)
                     /gene="yhhT"
                     /locus_tag="ACHOS5_002166"
     CDS             complement(17987..19036)
                     /gene="yhhT"
                     /locus_tag="ACHOS5_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9776982.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPRFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            19135..20385
                     /gene="yhhS"
                     /locus_tag="ACHOS5_002167"
     CDS             19135..20385
                     /gene="yhhS"
                     /locus_tag="ACHOS5_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9776983.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGVVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(20389..20946)
                     /gene="dcrB"
                     /locus_tag="ACHOS5_002168"
     CDS             complement(20389..20946)
                     /gene="dcrB"
                     /locus_tag="ACHOS5_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP9776984.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(21019..21684)
                     /gene="yhhQ"
                     /locus_tag="ACHOS5_002169"
     CDS             complement(21019..21684)
                     /gene="yhhQ"
                     /locus_tag="ACHOS5_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP9776985.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            21905..22150
                     /gene="tusA"
                     /locus_tag="ACHOS5_002170"
     CDS             21905..22150
                     /gene="tusA"
                     /locus_tag="ACHOS5_002170"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP9776986.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(22419..24617)
                     /gene="zntA"
                     /locus_tag="ACHOS5_002171"
     CDS             complement(22419..24617)
                     /gene="zntA"
                     /locus_tag="ACHOS5_002171"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP9776987.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSIAAIGALFIGATAEAAMVLLLFLIGERLEG
                     LAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(24691..25317)
                     /gene="yhhN"
                     /locus_tag="ACHOS5_002172"
     CDS             complement(24691..25317)
                     /gene="yhhN"
                     /locus_tag="ACHOS5_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP9776988.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            25458..25817
                     /gene="yhhM"
                     /locus_tag="ACHOS5_002173"
     CDS             25458..25817
                     /gene="yhhM"
                     /locus_tag="ACHOS5_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP9776989.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(25820..26089)
                     /gene="yhhL"
                     /locus_tag="ACHOS5_002174"
     CDS             complement(25820..26089)
                     /gene="yhhL"
                     /locus_tag="ACHOS5_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP9776990.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(26079..26675)
                     /gene="rsmD"
                     /locus_tag="ACHOS5_002175"
     CDS             complement(26079..26675)
                     /gene="rsmD"
                     /locus_tag="ACHOS5_002175"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP9776991.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            26825..28321
                     /gene="ftsY"
                     /locus_tag="ACHOS5_002176"
     CDS             26825..28321
                     /gene="ftsY"
                     /locus_tag="ACHOS5_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9776992.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            28324..28992
                     /gene="ftsE"
                     /locus_tag="ACHOS5_002177"
     CDS             28324..28992
                     /gene="ftsE"
                     /locus_tag="ACHOS5_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9776993.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            28985..30043
                     /gene="ftsX"
                     /locus_tag="ACHOS5_002178"
     CDS             28985..30043
                     /gene="ftsX"
                     /locus_tag="ACHOS5_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9776994.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            30288..31142
                     /gene="rpoH"
                     /locus_tag="ACHOS5_002179"
     CDS             30288..31142
                     /gene="rpoH"
                     /locus_tag="ACHOS5_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP9776995.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            31413..32516
                     /gene="livJ"
                     /locus_tag="ACHOS5_002180"
     CDS             31413..32516
                     /gene="livJ"
                     /locus_tag="ACHOS5_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP9776996.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGVIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32704..33087)
                     /gene="panM"
                     /locus_tag="ACHOS5_002181"
     CDS             complement(32704..33087)
                     /gene="panM"
                     /locus_tag="ACHOS5_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP9776997.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPDVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
BASE COUNT         7935 a         8634 c         9021 g         7600 t
ORIGIN      
        1 attcggtatt tattcgcaca tcaggcgata ataacgctat gtgatagtca tcaggtttcc
       61 ctgttacgga aacctgtgca acagaatctg tgaacctgta cagaatattt ttggaaaaaa
      121 caccagcgaa cggaagaaca tcgcgatgta tcacaagttg atggcaatca aatagacaac
      181 atgaatataa ctgttagtat ttaattgatt tcacttatga gtatgcgtgt ttcatttacg
      241 gccaggaatg ctgatgatga caacattact acccgttttc actaagcctt ccccattggc
      301 gctcaatgct ctgtgcgctg gtcgtatttg ccatttcctt cttatcccgg atggaagaat
      361 ccgctgaaca tcgttcggtg gcagttgtaa cacgcgtcga ttgcttacag ggattatggc
      421 ggatcgatta tggataagag taagcgccat ctggcgtggt ggggtgtcgg ggcactggcg
      481 gtggctgccg tcgtggcgtg gttgctgttg cgcccggcgg gtgtgccgga aggctttgct
      541 gtcagcaatg ggcgcattga agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt
      601 atcgacacca ttctggtgaa agaaggccag tttgttcgag aaggtgaagt gctggcgaag
      661 atggatactc gcgtgttgca ggaacagcga ctggaagcca tcgcgcaaat caaagaagca
      721 caaagtgccg ttgccgccgc gcaggctttg ctggagcaac gccaaagcga aactcgtgcc
      781 gcacagtcgc tggttaatca acgccaggca gaactggact ccgtagcaaa acgtcatacg
      841 cgttcccgct cactggcaca acggggggca atttctgcgc aacagctgga tgacgaccgc
      901 gccgccgctg agagcgcccg agctgcgctg gaatcggcaa aagctcaggt ttctgcttct
      961 aaagccgcta tagaggcggc acgcaccaat atcattcagg cgcaaactcg cgtcgaagcg
     1021 gcacaagcca ctgaacggcg cattgccgca gatatcgatg acagcgaact gaaagccccg
     1081 cgtgacggac gcgtgcagta tcgggttgcc gagccagggg aagtgctggc ggcaggcggt
     1141 cgggtgctga atatggtcga tctcagcgac gtctatatga ccttctttct gccaaccgaa
     1201 caggcgggca cgctgaaact tggcggtgaa gcccggctga tcctcgatgc cgcgccagat
     1261 ctgcgtattc cggcgaccat cagttttgtc gccagcgtcg cccagttcac gccaaaaact
     1321 gtcgaaacca gcgatgaacg gctgaaacta atgttccgcg tcaaagcgcg tatcccaccg
     1381 gaattactcc agcagcatct ggaatatgtc aaaacgggct tgccgggcgt ggcgtgggtg
     1441 cgggtgaatg aagaacttcc gtggcctgaa gacctggtgg tgaggttgcc gcaatgacgc
     1501 atctggaact ggttcccgtc ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg
     1561 gaaaaaccgt tgcgctgaac aatataactc tcgatattcc ggcccgctgc atggttgggc
     1621 tgattggtcc ggatggcgtc gggaagtcga gcttgttgtc actgatttcc ggtgcccgcg
     1681 tcattgagca gggcaatgtg atggtgctgg gcggcgatat gcgcgacccg aagcatcgcc
     1741 gtgacgtttg cccgcgcatc gcctggatgc cgcaagggct gggcaaaaac ctctaccaca
     1801 ccttgtcggt gtatgaaaac gtcgattttt tcgcccgcct gttcggtcat gacaaaacgg
     1861 agcgggaagt ccgaatcaat gagctgctga ccagcaccgg gttagcaccg tttcgcgatc
     1921 gtccggcggg aaaactctcc ggcggaatga agcaaaaact tgggctgtgc tgtgcgttaa
     1981 tccacgaccc ggaactgttg attcttgatg aaccaacaac gggggttgac ccgctttccc
     2041 gcgcccagtt ctgggatctg atcgacagca ttcgccagcg gcagagcaat atgagcgtgc
     2101 tggttgccac cgcctatatg gaagaggcgg aacgcttcga ctggctggta gcgatgaatg
     2161 ccggagaagt gctggcaact ggcagcgccg aagagatgcg acagcaaacg caaagcgcca
     2221 cgctggaaga agcatttatt aatctgttac cgcaagcgca acggcaggcg catcaggcgg
     2281 tagtgatccc accgtatcaa cctgaaaacg cagagattgc catcgaagcg cgcgatctga
     2341 ccatgcgttt tggttccttc gttgccgttg atcacgtcaa tttccgcatt ccacgcgggg
     2401 agatttttgg ttttcttggt tcgaacggct gtggtaaatc caccaccatg aaaatgctca
     2461 ccggactgct gcccgccagc gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa
     2521 aagatatcga tactcgtcgc cgggtgggct atatgtcgca ggcgttttcg ctctataacg
     2581 aactcaccgt gcggcaaaac ctggagttac atgctcgtct gtttcatatc ccggaagcgg
     2641 aaattcccgc gcgagtggct gaaatgagcg agcgttttaa gctcaacgac gtcgaagatg
     2701 tattgccgga gtcattgccg ctcggcattc gccagcggct atcgctggcg gtggcggtga
     2761 ttcatcgccc ggagatgcta atcctcgatg agccgacttc tggcgtcgat ccggtggcga
     2821 gggatatgtt ctggcagttg atggtggatc tctcgcgcca ggacaaagtg actatcttca
     2881 tctccaccca ctttatgaac gaagcggaac gttgtgaccg tatctcactg atgcacgccg
     2941 gaaaagtgct cgccagcggt acaccgccgg aactggttga gaaacgcgga gccgccagtc
     3001 tggaagaggc atttatcgct tatttgcagg aagcggcagg gcagagcaac gaagccgaag
     3061 cgccgcctgt ggtacatgac actacccacg cgccacgtca gggattcagc ctgcgccgtc
     3121 tgtttagcta cagccgccgc gaagcgctgg aactgcgacg cgatccggta cgttcgacgc
     3181 tggcgctgat gggaacggtg atcctgatgc tgataatggg ttatggcatc agtatggatg
     3241 tggaaaacct gcgctttgcg gtgcttgacc gcgatcaaac cgtcagtagc caggcgtgga
     3301 cgctcaatct ctccggttcc cgttacttta tcgaacagcc gccgctcacc agttatgacg
     3361 agcttgatcg tcggatgcgt gcgggcgata tcacggtggc aattgagatc cctcctaatt
     3421 tcgggcgcga tatcgcgcgt ggtacgcccg tggaactcgg tgtctggatc gacggtgcga
     3481 tgccgagccg cgccgaaacg gtaaaaggct acgtgcaggc catgcaccag agctggctac
     3541 aggatgtggc gagccgacag tccacacccg ccagccaaag cgggctgatg aatattgaga
     3601 cgcgctatcg ctataacccg gacgtaaaaa gcctgccggc gattgttccg gcggtgatcc
     3661 cgcttctgct gatgatgatc ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag
     3721 agctagggtc gattatcaac ctttacgtga cccccaccac ccgtagcgaa tttttacttg
     3781 gcaaacagct gccatacatc gcgctgggga tgctgaactt tttcctgctc tgcgccctgt
     3841 cggtgtttgt gtttggcgta ccgcataaag gcagtttcct gactctcacc ctggcggcgc
     3901 tgctgtatat catcatcgcc accggaatgg ggctgctgat ctccaccttt atgaaaagcc
     3961 agattgccgc cattttcggt acggcaatta tcacgttgat cccggcgaca cagttttccg
     4021 ggatgatcga tccggtagct tcgctggaag gtccaggacg ttggatcggc gaggtttacc
     4081 cgaccagtca ttttctgact atcgcccgcg gaacgttctc gaaagcgctg gatctgactg
     4141 atttgtggca actttttatc ccgttgctga tagccatacc gctggtgatg ggcttaagca
     4201 tcctgctgct gaaaaaacag gagggatgat gcgccattta cgcaatattt ttaatctggg
     4261 tatcaaagag ttgcgcagtc tgctcggtga taaagcgatg ctgacgctga ttgtcttctc
     4321 gtttacggtg tcggtgtatt cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc
     4381 gatcgccatt gccgatatgg atcaatcgca gttatcgaac cggatcgtta acagcttcta
     4441 tcgcccgtgg tttttgccac cggagatgat caccgccgat gagatggatg ccggactgga
     4501 cgccggacgt tataccttcg cgataaatat tccgcctaat tttcagcgtg atgtcctcgc
     4561 cggacgccag ccggatattc aggtgaacgt cgatgccacg cgcatgagcc aggcattcac
     4621 tggcaacggt tatatccaga atattatcaa cggtgaagtg aacagctttg tcgcgcgcta
     4681 ccgtgataac agcgaaccgt tggtatcgct ggaaacccgg atgcgcttta acccgaacct
     4741 cgatcccgcg tggtttggcg gggtgatggc gatcatcaac aacattacca tgctggcgat
     4801 tgtgttgacc ggatcggcac tgatccgcga gcgtgaacac ggcacggtgg aacacttact
     4861 ggtgatgccg ataacgccgt ttgagatcat gatggcaaag gtctggtcga tggggctggt
     4921 ggtgctggtg gtgtcgggat tatcgctggt gctgatggtg aaaggcgtgc tgggcgtacc
     4981 gatagaaggc tcgatcccgc tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac
     5041 gtcaatcggt atttttatgg ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt
     5101 gattctggtg ctgctgccgc tgcaaatgct ttccggtggt tccacgccgc gcgaaagtat
     5161 gccgcagatg gtgcaggaca ttatgctgac catgccgacg acacactttg ttagcctcgc
     5221 gcaggccatc ctctaccgtg gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct
     5281 gatagcgatt ggcggcgcgt ttttcaccat cgcactgctg cgattcagga agacgattgg
     5341 gacaatggcg taaggaaggg aaacttgact tcatattgtg atgtcatact attgatgtca
     5401 taaagtgaag tcataaggac atgccatgag aacccaggta caggctttac ggaagaaaaa
     5461 aaatacattg gatcaaatat ttaaatctcc tgttcctcaa gggattaagt ggtctgatat
     5521 agagtcactg gttaaagcat taggcggaga aattaaggaa ggaagaggtt cgcgttgtaa
     5581 gttcatacta aatatgagcg ttgcgtgttt ccatcggcct catccgtcgc cagataccga
     5641 taaaggcgct gtagaaagcg tgcgtgactg gttactaagt ataggggtaa aaccatgatc
     5701 aaactgaaga cgccaaactc tatggagatt gccgggcaac ctgctgttat cacttacgtg
     5761 ccagaactga atgcatttcg tggcaaattc ctgggcttgt caggttattg tgattttgtc
     5821 tctgacagca ttcaggggct gcaaaaagaa ggggagcttt ccctgcgaga gtatcttgag
     5881 gattgtaaag ctgcgggtat tgagccgtat gcgcgtacag aaaagattaa gacctttaca
     5941 ttacgctatc cagaatcgct gagtgaacgc cttaataatg cggctgcgca gcaacaagtc
     6001 tctgtaaata cttatattat tgaaacgcta aatgagcgtt tgaatcattt ataacttata
     6061 gcggcccaac aatttctgtt gcgccgctat atttttgtgg ctcttaagat gcctttctaa
     6121 tcaatcgatg cccctcattc atcatcagga ttttgctata cacatcttcc tcgacggcat
     6181 tttttgctga tgccatcacc cgtgccagat tcgcctcgtt gtgattattc acatacgctt
     6241 tgccgactgc ggtgataaac gctttgcgca gatcgctggc gatattcact ttagcgacgc
     6301 gataattaac gaaactgcgt aatatctccg gggcgatacc ggagccgccg tgaataacca
     6361 gcggcaccgg gctgacttcg gcaatgcgct tcagcagggg gatatcgatc cgcggtatat
     6421 cgtccaggcc atgaacgtta ccaatagaaa ctgccagcat gtcgcaaccg gtgcgttcga
     6481 caaaggtctt caccttttcc ggttccgttt tgcaatcggc ttcgctgacg tgatcatctt
     6541 ctttgccgag aatcgccccc agctccgctt caaccggcac gccataggat ttacagaaat
     6601 cgacagcttc ctgggtgaaa gcgatattct cttcaaaaga gaatgcggca ccgtcaatca
     6661 tgaccgaggt aaagcccgcg cgcaccgcct gtttaacatc ctccagggtt ttcccgtgat
     6721 ccagatgaag actgaccgga acatccattt tatcggcgtg acgtttcacg atttcatata
     6781 tgtaatcgta tcccgacagt tgtgcattgg tgggcgcaat ctgaatgaag ttgggtaatc
     6841 ccgaacgctc aatagcgttt aaaattgaaa ttgtcgtctc cagattggtg gtattaaacg
     6901 cgcccgctaa tacccgtttt tcctgaatgc ggtcgagcag tatccgtcca ttcactaatg
     6961 gcatattgaa aacctcatct tttaatatca gttaattatt cagcttcacc gaagcggtcg
     7021 cgaaataagg cggtgagagt ctgtatggcc tcgttttcat catcaccgtc gcagcgtatt
     7081 tccaccgttt ttccgccttt tattccggcg cgcataatat tcatcatact ggtggcatta
     7141 atatcattac cctcataatg cagcgtcact ttacagctat actttttggc ggctttcgcc
     7201 agacaggccg ccggacgtgc atgcaggcca gtactgtttc tgacctccac ggttttagtc
     7261 aacatagtgc tcctcccgat gatgtaatgg catcacgcct tcttgctgta atttcaactg
     7321 ataccagctt tcccatttgt gctgataacg ctgtaattga tgatatcttg cccgattcgg
     7381 taacaaacgt tgggctatgg taacttgcgg cggcggaata tccggtgccg cggttaataa
     7441 ggctccgaca gaaaccgcct cgctaaatgc aattgcttca accggacgct gtaaaatatc
     7501 tgcctttaat tgcagccaaa gggggttatg agtcgccgga ccgatcactt ttaacacgct
     7561 ggtgccaggc acattaaaac aggcaaagca gttggcaaat tccatggtta atcccagtga
     7621 gacgctggaa attaacatgt cgatatttaa cgtatcgcca agcccataaa ataatccgcg
     7681 ggtgtggcga tttttatagg gtgaacccga accgcgaaga tgcggaataa agacaggaat
     7741 gtcatcaagc gaccagttcc ccgccaggta atccgcatac ccacgattga gcgacacggc
     7801 gatctcctca tcggttagcc ggaacgtgtt acgcagccac tccagcgcga aaccgcccac
     7861 gggcagagaa gcaaacaggg tgaactggtt ggcaagcgag taacagccgt ttgccagctt
     7921 gtcacgtttg gcctgttcat ccagcgtcgg ttgtgtatcc agttgcagaa tgccttccgt
     7981 ggtgcccgtc gagttaagga tatcgcccgg catcatctgc aacgcccgcg cacccaccat
     8041 atggtcatgt ccggccagcg tcacgctgac cgattgcgag aacccaagcg ttttacagag
     8101 cgactcactg acccatccgg cgtgttcgcc tggctgcacc aatggcgcaa atgctgaaca
     8161 gggaacgtgt aacaacgccg ccgctttcgc tgaccattcg cctttgcgca cgtccaggca
     8221 cagtgtacgg ctggctaagg tgatatccgt tcgctgttcg ccagtcagcc gccagagcag
     8281 cacttccggt gcatgtagcc agcgcaggcc gcgacgattg cgcagcggat aatgctccag
     8341 caaccaacgc attttgaagg cagagtaatt gctgtgcagt ggtaacccgc aaatgtcata
     8401 aagtgccgca ctgtctgcct cgctaagcgt tgccagatac tcttcaccgc gacggtcata
     8461 ccatgccagc attggcgtca ggatctcgcc atgttcgtca agaaacaccc ctgactcgcc
     8521 aaaactggca atgcttatcc gcctgaccgg cagcggcgaa gcggcgttca gttgcgctat
     8581 cgcctggcgg acctcctgcc agagggcgtc gatatcgaaa tcgacatcgc cctgtgggga
     8641 gatctgcttt gccgtcacga atttatgcgc gcccaacgtc gttgcgtcca ggcaggaaaa
     8701 gcaggtgact ttgcaattgg tggtgccaat atcgataacg atagctgcgc tgttatcagg
     8761 cataaaccgt gctccgcgtc attcggaaaa tgccatacag taccagggcg ataaccggca
     8821 ccagaccaat ggcgatgata ttgccgttga agatttccag cagccagaag cggaacgggt
     8881 taccgccatc aaggaaactg gaaacctggc cgctggagcc ttcggcaaat ttgaagttaa
     8941 cgtctttcgc catgccggta ataaacggtg cgattttagt cgcaatccac aggtcggcaa
     9001 taatcaccgg gatcgccgcc agaacggtgc ggaaaatatt gccacggctg gctaaagcaa
     9061 tcatcgacgc cattaccgcc aggttggcaa ggtcaccaag cggcaggact tcattacctg
     9121 ggaggacaaa cgcgataaag accgagattg gcgtcagcag cagggcggtg gagataatcg
     9181 ccgggttacc tacggcaacg gcgatatcca ggccgatata gagatcgtca cggtcagggt
     9241 atttggcatt gagatacttt ttaatggctt cagacagcgg taataaacct tccatcagga
     9301 tgcgtaccat gcgtggcagg atgaacatca caccgccaat gctgatgcca agcagcaaga
     9361 cttttttgaa atcgtatccg gcaattacgc cgagcagaat gcccagaata gtgccaacca
     9421 tcatcggttc gccgaagatg ccaaactttt tctgtacggt ttccgggtcg atatgaatac
     9481 gattgaggcc agggatatgg tcgatgattt tgtcgaccaa cagaccgacc ggaaagaaca
     9541 ccaccgaaga gagcgtcggc aaagagatcc cttccaggcc aaagtatttt tgtaccagtg
     9601 gcgcagacca gtcggcgagt ttaagtacta ccaccgcagc aatcgccgct gccagcaaac
     9661 caaagaacag gctgccggtg ctgtaataaa ccagcgtacc ggcaagggca aaatgccagt
     9721 agttccacat atctacgtca acggtgcggg ttttatttaa cgccagcatc gccacgttaa
     9781 tcagaatagt caggggaata acaaacgggg caattggcga accccatgta atagcggaaa
     9841 gcggcggcca gcctaaatcc accaccggta aattaattcc ggtgcgttca accatcgcct
     9901 gggccgctgg cccgacattg ctggtaagaa tatcgaaaat ggcataaata ccgacaaaac
     9961 caatgccgac cgttaatgac gaacgtaagg ctttagcggg ttttacttta aaaaagagag
    10021 caagaataaa taacaccaac ggcaacatta ccgtgggacc aaatcccaat atatattgca
    10081 caatattata gagggtatgc gcgatatcgt tcataataaa cctctttttc aggcgagagc
    10141 gcacctcgcg ttagcgcctg atggagtggg ttgaaattaa ttattattaa ttacttttta
    10201 aggatcgaaa caatttgcgc cagtactttc tcttcgccaa taccggtaat aatcggcaag
    10261 ccgttaacca ccgggacgtc gagttcataa ggcactttag tcgttgagac taccagtgct
    10321 acgccgtcag tatttccagg taaagaggca acgttggttt gggaataatt gacgttaaga
    10381 ccgtgctctt tacaatattc catgactttt tctgttactg ccgtggatgt ggcaataccc
    10441 gtggcgcaga caaataaaat ggtttgactc atatttcacc tcgttctttc attttgttta
    10501 agattgctgt gtgtatttct gttgtttcca tcgtaagcag ggcgcgcata aagggcgtat
    10561 cctgaatcat ctccataatc cagcccaggg catttaattg tttattactt tcgcctaagg
    10621 cgagcaaaaa aatcaccctg acaggtacgg gatcagagtc gccgcccata tcggcaaaca
    10681 ctaccggttc cggcaggata ccgacggcaa ttgcattgtg cctgacatgt ttatggtccg
    10741 tgtgcggaat ggcgacacac ggtggttcgg taggcagtcc cgttgggtac gtcgcctcac
    10801 gttccagtac atgatcggca aaatcaggga ccaccagttc ggcggtaacc aacggttgcg
    10861 ctaactgatg gatgatctgt tcaacgctta accctgccgg aagtcgttga ataaaatttt
    10921 cctgaaactg tatgccttgc attttattcc tcttaaaaaa gtgtacttaa ctcacttccc
    10981 gccgttgcag cacggtgcca agatagtatt tatttccttt taaccagacg ttgccaaact
    11041 caacgggtaa tcctcgggag aaaaagacta actgctccag atgcaaaacg ggtgcatctt
    11101 cactgatatc taaaaaatga ccgcgttcat taccaattaa tcgcgcggca tagcgacttt
    11161 ccgagtaacg aatttttctt tgcgacaaac tttctattgt tggaaataaa ttgtgttgat
    11221 taaaatcgat ttccacgatg ccggggcata gctcaatatt gatacggttc tctatcagca
    11281 tcgctttttc atcaccaatt gaacgtaaac gctcaaggta aagaatatcc tgaccgggcg
    11341 ttattcttaa tttctctgcc acataacgat tagccggttc aatccgtgac gtaataactt
    11401 cagtggtaaa atgtattttc tggctttcca gcgattccgc gaatgacaat aatccttcac
    11461 ctaacggata tgccacgttc tcttttttga caaatgttcc cttcccctgg acctgtatca
    11521 acacgccttc ttttaccagt tgggcgaccg cttttttaac ggttccccgg ctcacttcca
    11581 gcatatccat aatctggtat tccgaaggaa tgcgatcgtc ttccaccaga tccccggtat
    11641 aaatactttc tcttatccac tccaccactt gttgatagag cggtatttgt gacgacttac
    11701 tcaatgattt catggcgcgc acctgaacat ttacagttgt ctatggacaa tcatggacaa
    11761 cttcaagcgt atagtaggtg aacaggcgaa aaagaggatc gaagcgtctc acattttagg
    11821 ggaccaaatc gtgatcaaaa aaatgtgacg tgagtcgata tttggcagaa gtctactgcg
    11881 ccgtcaggaa ataacggcgc gttgggaggt gttaatcttc cttcggcagg cactgcaaat
    11941 gcccgtgccg cacgccgcgc tgggcgatga cgtcatcggc aaaatgctgc acgtcgccca
    12001 tgtcaccttt caacacggcg atttccagac agtcgtcgtg attaatgtgc acatgcagag
    12061 tagcaaccga gagatcgtgg tgatgatgct gggtggagac aatacggctg gctaagtcgc
    12121 gtttttcgtg ttcatacaca tacgacagca ccgcgaaacc ttgtgtgccg tgttgttgag
    12181 tggcttcctg cgccagggcg ctacgcagaa tatcgcggat cgcctcggaa cggttgttat
    12241 aaccacgacg ctggctcagg ctgtccagcg tctccagtaa atcgtcatca agcgtgatgg
    12301 tgactcgttg catttgcgtt aaaccttttc tgtggtgcga cggcgcacgg ggaatgcggg
    12361 taataccgcg ttttgtagca cacgtccggc gtcagaggaa aaggttaatt tatctcccac
    12421 cacctgggtt tcgacgattt gtccgttgtc catcaccatc acccgctgac agaagcgttc
    12481 caccaggcgt aagtcgtggg tgatgaacag gcaggcggtg ccaaactgtt gttgcagctt
    12541 tttcagcagg cgaatgacgc ccgcttgtaa catgagatca aggttcgaaa ccgcttcatc
    12601 cagaatcagc agtttcggtt cgaccgccag cgcgcgtgcc aggcagacac gctggagctg
    12661 gccgccgctt aactgcggtg ggcgtttgtc gagcacgctg tcgtcgagat cgaccgcctt
    12721 cagcatttca ctggcgcgcg ccagttgttc ggcttttttc agcgatagca ggtggcgcat
    12781 cggttcacgc aggatctcgc gcacggtttt gcgcggattc acggcgctga tggagtcctg
    12841 aaacaccatt tgaatatcac ggcggaacgc tttacgttgc gcgcgattga gcttcgccaa
    12901 cggttcgccg cgccagctaa tatttccctg tgagggcgac tctaacccta ccagcagccg
    12961 cgcgagggta ctttttccgc agccgctgcg ccccagcagg gcgacggttt caccgctttt
    13021 cagggccagg gaaacgttat tcagcaccgc ctggtgttga tgtttgccgc taaatccacc
    13081 gtgcgcgtag tgatgggaaa ggtcggagac gttaagtaaa gtcatgatgc cagctccata
    13141 ccgtagaggg cgagatgagc ggaaaccagg ctgcgcgtta ccgcatgttt gggggcgtta
    13201 aacagcgttt ctacatcgcc ctgttcaaca atcttaccgt gagacattac cgccacatcg
    13261 tctgccagac gcgctaccac gcccatatca tgggtaacca gcagcattcc cggtgcttgt
    13321 ttttgcataa tgctttccag cagatcgagg atgcgcgcct gtgctaccac gtcgagatcg
    13381 gtggttggtt catcggcgat gataaacggt gattcgcaca gcactgccat cgcaatcatc
    13441 atgcgctgca acatgccgcc gctcatctcg aacgggtaga gcttcagcac gcgcgcggcg
    13501 ttttccagcc ccaccgcttc tatggcagcg gtaagcgtag cgtcatcggc gggtttcccc
    13561 agcgccaggc aggtttcacg cgcgtgggtg tgcatggtgt gcagcggatt aaaggcactg
    13621 cgcgggttct gcatgatggt ggcaattttg atgccgcgca gggcgcaggg agaaaccggt
    13681 tttccatcgg ccaaaatttc ccccgccgtc tggcgaacgc cagcaggcag aatgcccagc
    13741 gtcgcggcgc aggtcagcga cttcccgctg ccactaccgc cgactaacgc cagcacacgc
    13801 ccacgttgca gggttaacga tacgccgtgc accagcggct gcgcggcctg tagcgcgata
    13861 ttacggagtt caatctgttg tggcattagt gtgcgtgctc cgtcaccaga tgagggtcca
    13921 gatgatcgcg cagtgcgtca cccaccaggt taaaggccat cacgctgata aacagcgcca
    13981 gccccggcca gaacatttgc agcggctggg tccagatata ctgacgcgcg tcgttaatca
    14041 tcacgcccca ttcggcggtc ggcgcggtca cgccgagacc gaggaaggac atccccgcga
    14101 cgtgtagcat catatgaccg atatccagcg tcgccagcac cagcagcgag gggatcaccg
    14161 cacctgccag atgatcaata aacacccgca catggcccgc gccagaaagc cgtgatgcca
    14221 gcacaaactc gcgctggcgc agtgaaatca ccaggctacg caccatgcgt gcataccacg
    14281 cccagtgcga cagggcgatg gctataatca cattggtcaa cccggtgcca agcacgccga
    14341 ccataaagaa cgacagaatc gaggttggga aggtcataaa catatccgcg acgcgcatgg
    14401 tggcctgatc aacgcgcccg ccaatcagcc cggcgctgcc gccaataatc agccctaatg
    14461 tcagcaccaa cagcaggcag gccattaccg agccgagcga cacgcgggtc gctgccatca
    14521 gccgcgagaa aatatcgcgc cctaaatgat cggtgcccag ccagtgctgc gcatccggcg
    14581 agagcaggcg cgacggcaaa tcaatagcct gagggtcata cggcagccac cactggctgg
    14641 tgagcgcaat cagcgccagc agggcgataa tgatcagcgc caggcgaacc gaccagcggg
    14701 aggagaggaa aaagttcacg cgtgcgctcc ttcatgacga cgaatacgcg ggtccagcgc
    14761 ggcgttgagc aaatcgacaa tcaaattaca gaccacaaaa accaccacca tcatcagcgt
    14821 aaagcactgg atcaccggat agtcacggtt aaaaatcgcc gacaccgcat agcgcccgac
    14881 gcccggccag gcaaagatgt tttcgataat catcgtcccg ccaatcagtt cgccaatgtg
    14941 catccccacg gcagtgatca tcggcagcga ggcattgcgc agaatatggc ggcgttcggt
    15001 ctgtttgtcg ctcagaccac gcagtcgtgc ccaggtgacg tgacgctgac cggcgacgtc
    15061 cagcatactg gcgcgcagca aacgcgcgtt aatcgccagc gacataaagg cgatggatac
    15121 cgcaggcaaa atgatgtgct gccagccgcc gtaacccatc gcgggtagcc attgcagata
    15181 caccgaaaac gccatcacca gtaaaaacgc cagccagaag ttaggcatcg acacgccgag
    15241 aaacgcgatg aaacggacgg cgaaatcggg caagcgatcg cggtggcgcg ccgcccagat
    15301 accgagcggc acggaggtga gcagaattaa taccagcgcc gcacccgcca gttccagcgt
    15361 ggcgggcagg aaattcagca tatcgtccag caccgggcgt tggctggcga atgagatacc
    15421 aaagtcaaga tgcagcgcct tccacaacca ggtgccatac tggacgtaca gcggctgatc
    15481 cagccccagc atagtgcggg tagaggccag catctccggc gtcggcggca ggttagacag
    15541 acggagataa tcgagcgccg ggtcaccggt gccgaggcgc agcatcagaa aaatgatcac
    15601 cgaggcggcg agcaccatcg ggatcagcag cagaaagcgg cgtaatacat aacgcaacat
    15661 taaggtttca ccggtttaat ctgttcgaac gggatttcgg tggcgatcgg cgcgtagggg
    15721 atgttaccca gttccggttt tgaaaccacc atcattgaga tgtaactgat aggcagataa
    15781 accgcctcgt catgcagacg ggtcagaatg tcgcgataca gcgcctgacg ttgcgtttcg
    15841 tcatgggtcg ccagcacttc gccgatctct ttatcaatca gcggcttgtc ggctaaacct
    15901 tgctgtgcct ggaagtcagc gtgcgacggt acgcgcattg aactcaggaa ggcgtgtgga
    15961 tcgtatggcg cgccccaggt gcggtggaaa atcatgccaa aacgaccgtc gcgctgacga
    16021 gcatagatac tgctctcttc ttcgccaatc agcgagacat ctgcgccaat ctggcgcata
    16081 tcagcctgaa tgatttccgc catcgatttg cttaacgcat cggtgccgat gaacgacagt
    16141 tcaatgcgca gcggctgacc atttttctcg cggatgtctt tgcctgcagg cagcgtccaa
    16201 ccggcttttt ccagcaacgc tttcgctttc tgcggatcgt actggcgcgg tttcaggccg
    16261 aggttggcgt agggcacaga aggggcaaac agggtgtcgg cgacctgctg ggtaccatac
    16321 aacgcgttat caatcagcga ttttttgttc accgcgtaat taagagcttc acgtactgcc
    16381 agctcgttgg tgggggcttt ggcggtattg agcgccagca tcacggtttc gatcggctgt
    16441 gacagttgag tgtggtaagc cgggttctgg ctaaagcggg cgaaggtatc gagcggtaat
    16501 aacccttcgt ttccgtacag caggtcgata tcgccagttt caaacgccac cgcgcgggta
    16561 gtcgggtccg ggatcacgtt aaaggtgatc tttttaatcg ccggcttttc gccccagtag
    16621 ttttcgttac ggacgaagac atcgtactga ttcagtttcg attcctgtaa aacccacggc
    16681 ccggtgccaa tcggcgcttt aattccgttc atggtttcgt ggtttttaaa ctgcgagggt
    16741 gcgataaaac ggaaaggacg gggcagggcc agttcttgca ggaaaggata gtaggcgctt
    16801 ttcagggtga tttgcagctc gtttttgctg agtgctttga catcaacaat ctggtttgcc
    16861 agctccagcc aggcgtgacg ttgacggtta tcgagcactg cacggaagtt ttctgccgcc
    16921 gcctctgcat caaacggttc gccgttggag aacttcacgt catcacgcag ggtgaaggtc
    16981 caggttttac catcttctga atgagtccag ctttttgcca gccacgggat caccgaaccg
    17041 tctgcctgat atttcaccag cggttcataa accatgctct gggcgaacat ttggttaggc
    17101 gtgtaaaggt gcgggtttag tggcccgaca ttcaccggcc aggcggtggt gatttcatct
    17161 ggtgcagcgg catggacgat aaaagacgca caagccagca gcgcaaatag agtgcggcgg
    17221 agtgtagaga acatggtaac cccaatggat taaaatagat ggcggaaata agtatgacga
    17281 ttttaagtat tcgtcatact gattacctgt tctggatcaa tagatgggca gtcgggtggg
    17341 cggatcagtt aactgaatcg atccattgca cactgtcggc ggtgagcgta aagggggtag
    17401 gggtgcagat cgccaggctt aaattttcta gctgacaatg gctgactgac agcgaggact
    17461 gataggtgct gtctacgctg acgatttgcc aggcgcttcc gccacgctgt ttaacgatgg
    17521 cttctttgcg cgtccagatg cgccagaaca tttccagttg ctgatcagga tgcacggcgt
    17581 ccatctcggc atgttccccg aggctgaata cggcgttcgc cagccagcgc cagttggcgc
    17641 gcgggcgaat cacttcgata tcgcagccga cttcgccttc atcgctcaac agcagggcga
    17701 tatcgtcacc gctatggctt aagttgaacc atagcggcgt ttccggcgcg aatgcaggtt
    17761 tgccttgttc gccatagatg atctccggta gcggggaaag cgtgtgcgaa agcaatgcac
    17821 gccccgccag ccagcgttca cgtcgtggac cttgcggtgc ttgctcgcgt aaacccggtg
    17881 gcagaggagc tgcgcttaag gtcgaaactt tccccagaac tatccgatac atatcagggc
    17941 caacgtttaa tggaaaatga aagtgcgtat cgtaacactt gtcgcctcat cccggtaacc
    18001 gacttttcgg tctgcctggc cccagtaaaa tcgccagttt gctgccgcct ttggtggttt
    18061 ccatccagat tttacacacg ctggttaatg gcaccgaaag tagcatccct accgggccga
    18121 gcagccatcc ccaaattaac aatgaaagaa ataccaccat ggtggacatc cccaggcgat
    18181 ggcccatcat ccgtggttct aaaatattgc cgatgaccat atggaccact aaaaacaatg
    18241 cgccgaccag aatacattcg taaataccat taaatagcag cacctgaatc attggcggta
    18301 cggcggaaat taccgcaccg atattgggca cgtagttgag caaaaacgcc agtactgccc
    18361 acatcagcgc aaactgcacc cccatcagct ccagccccag ccagacgatt atgcctgtcc
    18421 ataaactgag tagcgtcttt aacgccagat aatgcgaaac gcctttaagt gcgcggtgta
    18481 gtccggcgat gtgaatctgt ggattattca acgcgaaacg cattttgtaa gggacgtggc
    18541 gcacttcaaa cagcataaaa actacggtca tcaccagcaa aagcacgctc gccattgccc
    18601 cggaaagccc ggtcattagc gccgtggtga aggtaaccac tttttccgag tccatccgct
    18661 gtagtattcg ctccggcgac atatgcaaat taagaaaagg caacatctcc tgcaatttaa
    18721 aaagtttgcg cgtcagctcc ttattaaacc tcggcagcat agagataaat tcgttaaacg
    18781 atgccgccag tacgccgacc agcgcggtga gtgcgatcag catcaccaca accacaatcg
    18841 taatggcaac ggggcgttgt accccccgac gaataaacca ggtgacgagc gggttgagaa
    18901 cgatggcaaa aaacagcgcc agtaatagct gcacaatgat atctgccgct gcgtgaatgc
    18961 ccgcgaggat cactaccagc gaggccaact tgagcagaat gtgcatgccc gttttatcgg
    19021 gttgaggggt ttccattggg gcttccttgt gactttttgt attaagtgta gcgggagtca
    19081 cgccagcaat attctgattc ttactgctga ttttcacgtc agcacatggt aaaaatgaaa
    19141 cactgttgta aaaatgtggt gatcctcatg cccgaacccg tagccgaacc cgcgctaaac
    19201 ggattgcgcc tgaatttgcg cattgtctcc attgtcatgt ttaacttcgc cagctacctt
    19261 accatcgggt tgccgctcgc tgtattaccg ggctatgtcc atgatgtaat gggatttagt
    19321 gcattctggg caggggtggt tatcagcctg caatattttg ccaccttgct gagccgtcct
    19381 catgccggac gttacgccga tttactggga cccaaaaaga ttgtcgtctt cggtttatgc
    19441 ggctgctttt tgagcggttt gggatatctg acggcaggat taaccgccag tctgcccgtc
    19501 atcagcctgt tattactgtg cctggggcgc gtaatccttg ggattggaca aagttttgcc
    19561 ggaacgggat cgaccctgtg gggtgttggc gtggttggtt cgctacatat cgggcgggtg
    19621 atttcgtgga acggtattgt cacttacggg gcgatggcga tgggtgcgcc gttaggtgtc
    19681 gtgttttatc actggggcgg attgcaggcg ttagcgttaa tcattatggg cgtggcgctg
    19741 gtggccattt tgttggcgat cccgcgtccg acggtaaaag ccagtaaagg caaaccgctg
    19801 ccgtttcgtg cggtgcttgg gcgcgtctgg ctgtacggta tggcgctggc actggcttcc
    19861 gccggatttg gcgttatcgc cacctttatc acgctgtttt atgacgctaa aggttgggac
    19921 ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg tcggtacgcg tttgttattc
    19981 cctaacggca ttaaccgtat cggcggctta aacgtggcga tgatttgctt tagcgttgag
    20041 ataatcggcc tgctactggt tggcgtggcg actatgccgt ggatggcgaa aatcggcgtc
    20101 ttactggcgg gggcggggtt ttcgctggtg ttccctgcat tgggcgtagt ggcggtaaaa
    20161 gcggttccgc agcaaaatca gggggcggcg ctggcaactt acaccgtatt tatggattta
    20221 tcgcttggcg tgaccggacc actggctggg ctggtgatga gttgggcggg cgttccggtg
    20281 atttatctgg cggcggcggg actggtcgca atcgcgttat tactgacgtg gcgattaaaa
    20341 aaacggcctc cggtggaaat acctgaggcc gcctcatcat cttaaaactt actgaataac
    20401 cagcgtatta atgatgtttt ctgcggtggt ctgtgctttt tgctgattgt cagcgggcag
    20461 cgtaatttgc atggttagca gttgattacc cacgttaccc agaataacgg aagagtacgc
    20521 cgtctggcct ttcgcggaga taatgctgtc taactgctgc attttgtgac ctttcagctc
    20581 aatggcttta ttggttacca cttgcagctg cggatcgcgg ctacgttgct gatcttccag
    20641 acgcttcgcc agcaccgcca gatcttcttt cggatcatcg cccatgatga cgatgactgc
    20701 tttctgcccg gtggcgtcgg accagacatg catgttattg gcctgcgttc ccagcttacc
    20761 gctctggtcg gtcatatccg ctggcagcga gaaacttaac ttgccatcaa gtaggttgac
    20821 gggattcccg gtcgcgttac tttctgcgac cgaaccctgc gccgtagcgt tagtgtcttt
    20881 atcatcacag gccgcaagcc ccataaccag caggccaatt ccgacatatt taaccagatt
    20941 gcgcattgac ttcttccttt cgataaacgg ccataacggc tcattcatcc atcttatcac
    21001 aactctgata acgaaccttt aactcgcctg caaagcgttg atttcggatt tatctgccag
    21061 tcttttcaac agcatattga gtagtacgcc atacattggc aggaagaaaa cgatactgat
    21121 taacactttg aaacagtaat cgaccagcgc gatttccatc cagtgttcag ccataaaggc
    21181 atccgggcta cgccagaagg caatgaagaa gaaggccagc gtgtcgctga cgttaccgaa
    21241 cagtgtggac gctgtcggtg ccagccacca gtgacgactc tgacgcaggc ggttaaaaac
    21301 gtgcacgtcg aggatctgcc ccagcgcgta ggccatgaaa ctggcggtgg cgatacgggc
    21361 gacaaacagg ttgaagtggg cgagcgcgcc gaatccttgc catgacccca tataaaacag
    21421 cgatgagatg acgtaggaga tcagcaatgc ggggatcatt accgcaaaga tgatgcgacg
    21481 agccagcggc gcgccaaaaa tacgcacggt caggtcagta gcgagaaaga taaacggaaa
    21541 gctaaacgcg ccccaggtgg tatgaaaacc aaaaatggag accggaagct gaaccagata
    21601 gttactggag gtgatcacca gcagatgaaa taacgataac cagaacaacg ccttatagcg
    21661 ttgcgtttgc gagaaaacgt tcatattgta cctttttgat taaccattgg ggtgagggaa
    21721 cccaatacgt acgacacgtc tgttacctta acataataac gtgctgaccc ttttttcgag
    21781 ccgccgcatg atactgcttt acgttgacaa tgcaatggtt aatttttacg caatcgttaa
    21841 cctggtttgc ttacggactc acagggcgta aactagcgcc gtttttttat gtgatgagaa
    21901 gaaaatgacc gatctctttt ccagccctga ccacacactc gacgcacttg gcctgcgctg
    21961 cccggaacca gtaatgatgg tgcgcaaaac agtgcgcaat atgcagccag gtgaaacgtt
    22021 gctgattatt gctgacgatc cggcgacgac ccgtgatatt cctgggtttt gtacctttat
    22081 ggaacacgaa ctggttgcta aagagacgga tggactgcct tatcgttatt tgattcgtaa
    22141 aggcggttga tgggggaaga tgggcttcga tgccgccttt tgccctcacc ctaaccctct
    22201 ccccagagga gcgaggggac cgatcgctct caatgttgct gcttgaaacc ggtttgtagg
    22261 ccggataagg cgttcaagct gcatctggcg atacggtgca caatgcctga tgcgacgctg
    22321 tcgcgtctta tcatgcctac gagttcgatg ctctgtgtag gccggataag gtgttcacgc
    22381 cgcatccggc attgttggac cgtagactga tagcctcttt atttcctgcg caataaccgt
    22441 aacgcattcg ccgtcaccag caccgtcgca cccgtatctg ccagcactgc cagccatagt
    22501 ccggtcatcc ctaacagcgt ggtgacgagg aagatccctt tcagccccag cgcaatggtg
    22561 atgttctggc ggatattggc gtgagtggcg cgtgccagtt caatcatttg caccaggccg
    22621 cgcaggtggt tatgggttaa tgctgcatct gcggtttcca gcgccacgtc tgtgccgcta
    22681 cccattgcaa tcccgatggc ggcagctttc atcgctggcg cgtcgttaat accatcaccg
    22741 accatcgcca gcggcgcatg ttgattcagc tcggtcaccg ctttgacttt atcttccggc
    22801 aagaggcccg ctttaaattc cagccccagt tccccggcga ttgccgctgc tgcgcgtgga
    22861 ttatcgccgg tgaggatcac ccctttgaca cccagcgcgt tcagttcact gatagcagtt
    22921 gcagcatcgg cgcgcagggt atcctgcaac gcaatgacac caagcacgtc atcattacgt
    22981 actaccagca ctaccgtttg cccggcgctt tccagttcat taatcaaacc agcaaatgca
    23041 tcagcaggat gttttccggc agcgcatatc aacacgcgct caccgttaac ctgcgcttca
    23101 atgccagacc cgaccagcgc ccgctgtgat tgggcggtgg gaatggcgag ttcagcaacc
    23161 tgtgcttcgc gcacaatggc ctgtgccagt ggatgcgtcg cgccttgctc gaccgccgcc
    23221 gccagtgtca gcagttcaga ttcactaata cccgttgccg gatgaatcgc ggtaacgcgc
    23281 ggtttaccga cggtcagcgt accggtttta tcaaacgcca cctgggtaac acgacccaac
    23341 tgctccagcg ccgcgccgcc tttaatcaac gccccacgac gcgctgctgc cgccagcccg
    23401 gaggtaatcg ccgcgggcgt ggagataact aaagcacacg ggcaaccaat cagcagcagc
    23461 gtcagccctt tataaatcca ctcctgccag ctggcggcaa acagcagcgg cggcaccagc
    23521 gttaccagca gggcgacggc cataatcgct ggcgtgtaaa tacggctgaa acggtcgata
    23581 aaccgctcaa taggtgcgcg gcgctcttcg gcttcttcaa tcagtttcag aatccggtca
    23641 atggcgctgg cacccggttc tgacagcact tccagcgtta ccagacggtc tacgctggtg
    23701 gcgcctgcag gaactttatc gcccgtcgcg cgctccacag gaatggattc tccggtcagg
    23761 gcactttcat caaaactggc aaacggtgag agcagtttac cgtcggcagg caaacgccca
    23821 cctgccgcaa cttcaatcac atcgccaggc cgcaggctgt ttattgccac ctcttcacgc
    23881 tcaccgttac gcagacgcgt ggcggtttct ggcttcagcg ccattaacgc gctaacgccc
    23941 tgacgcgcgc ggctggcggc caagccttcc agtcgttcac caatcaaaaa cagcagcaac
    24001 accatcgcag cttcagccgt tgcgccaata aacagtgcac caatagcggc tatgctcatt
    24061 aaggtttcaa tggcgaagta gctgccagat ttgatcagcc gtaatgcctg acgagcaatt
    24121 gggtacagcc caaccagcgt ggtcgcgata aacgccagtt gaccaaacgg atgattgaac
    24181 tgctccagcc cccaactgat tgccatcatc acgataagcg taatcagcgg cagattctct
    24241 ttcaggcgtg attcttgtgg ttcgtcggag gcctgttcat cgcgcaggga atagcccgct
    24301 ttttgcaccg cagattcaac ttgtgcccgg atgtcattgt cggcatccac caccagtttt
    24361 tcggtggcga acaacacctg tacctgattc acgcctgcaa gctggcgcac ggcattttcc
    24421 actttgcgcg cacaggcggc gcagtccatg ccgctgactt tccagctata gcgggtgccg
    24481 gagacgtttt cagagagagt tggcgtgctg gaacatgcgc cgtcgcagca acagtcgttg
    24541 gtgttctgta ccgtagttag cggtttgaac gcagcaaatt gaggggcttt cttgccgtga
    24601 ttgtcaggag tcgacatggc atcctccggt taagtttttt ctcattaacc gaaggataca
    24661 ctctggagtc gactccagag tcaagtttta tcagagatac agcgagcgga cgatgaggaa
    24721 gtgaccagca aagtagcagg ccgcggcaat ggcgttatcc gcacggaagc gtcggcgata
    24781 gtgactaccc agccagacaa agttactgat aaacagcaac gaagcgccga caaacgcaga
    24841 gagcgccgga gcggtcggac ggaagaacca cagttcacct gccagccaca ccatcaccag
    24901 cgtcatgccg ataaacgtgc agataggcca acgatactct tccagacgcg tccagataat
    24961 cgccagtaac agcgcgccca gaaccagcag caccaacggc agaggccaga agaaagagag
    25021 cgtcatctga ctggcgaaat agatggtgta cagcaggtgc gagaggaaaa acgcgccgat
    25081 ggcgtacatc agacgttgac gtggcaacag ggttagcgca tcgcccagca gtgaggcaca
    25141 cagccccgcc agcaccagat agctaatggc gtcgaacatc ggcgcttgcc aggccagcaa
    25201 cagcaggaga agaagggtta acggtttaaa cacccagcgt tgccaggttg gcccacgata
    25261 cgatgcatcc acagatagcc atgcggaaag acagacagcg ataaacgacc aaagcatctt
    25321 agttccttga attcgttatt ttttcgtaag cggaatgcct acggtcttgt ttccagttta
    25381 gtggcgaggg tggacggatg acaacaccat aactggcagg gtatgcttat ttccgcattt
    25441 tccgatgagg gataaagatg agtaaaccac ctcttttctt tattgttatc attggcttaa
    25501 tcatcgtcgc cgcatcgttt cgttttatgc aacaacgacg ggaaaaagct gataatgata
    25561 tggcaccgct ccagcaaaag ctggtggtgg tgagcaacaa gcgggaaaaa ccgattaacg
    25621 atcgccgttc gcgccagcag gaagtgactc cggcaggcac cagtatgcgc tatgaggcaa
    25681 gcttcaaacc gcaaagcgga ggaatggagc agacgtttcg cctcgacgcc cagcagtacc
    25741 acgccctgac agtgggtgat aaaggtacgc tgagctataa aggaacgcgc tttgtcagct
    25801 ttgtaggcga acaataaggt tattttttaa ctttaaattt cttctgccag accagcagtt
    25861 caaacacgcc gaaaaggaaa atccgcacct tttcggcggt ggtcatctgc gggccatctt
    25921 tcggtaaagt ggatttcaat agcgccagct gcataccatg catcagcacg gtaaaaatca
    25981 gcgccacgtt aacgaaaata ttcagcgggc gtgggaaggg atgcaccagg ttgaggatta
    26041 aaaatcccca aacacagagc attaacaaac gaccaatatt aatcagcatc actttctcct
    26101 tgtgcttcgc gttgatacag ccgataagcc acctgacccg ccactttttc ccgatgtaat
    26161 gaccagtttg ccggaacagt gggcagaccg ttttcgactt cgctttcgac ataaatcagg
    26221 gcttcgtcag ccagccagcc gttatcttcc agtaaattta tcgtctcttc taacaagcca
    26281 cggcggaacg gtggatcgac aaacacgata ttatgcggtg tgcctttttg cgccaggaat
    26341 gacatcgcgt tgctgttcac cacgcgtgca ttgcctgctt ttagtgtcgc cagattctta
    26401 attaactgct gagaaaccgc acgatccatc tcaatcaacg tggcccccgc agcgtagcgc
    26461 gataacgctt ccagccccag cgcgccgctc ccggcgaagc aatccagaca ttgggcgtca
    26521 acaatgaccg gagccagcca gttaaacaac gtttcgcgta cgcggtcggt ggtggggcgc
    26581 agacctggac tatcaggaac cgggagttta cggcctcgcc actgcccgcc aataatgcgg
    26641 atttggccgc tgccggaatg attcggtttt ttcatgataa attgctcaat ccgccagata
    26701 acacatactt gcaggcggtg atgtgaatta agttaagtga tagactattt catcattttt
    26761 ttagctgcta tgtacatagc gttaacgctg tgccatgaag caacagcgag gagtgtagtc
    26821 gcaaatggcg aaagaaaaaa aacgtggctt tttttcctgg ctgggctttg gtcaaaaaga
    26881 gcagaccccg gaaaaagaga cagaagttca gaatgaacaa ccagttgtag aagaaatcgt
    26941 tcaggcgcaa gagcctgtga aggcttctga acacgccgtt gaagagcagc cgcaggcgca
    27001 tactgaagcc gaggcggaaa cttttgctgc cgacgttgtg gaagttactg aacaggttgc
    27061 tgaaagtgaa aaagcgcagc ctgaagcgga agtcgttgca cagccggaac cggtcgtaga
    27121 agaaacgccg gagccagtgg ctatcgaacg tgaagaactg ccgttgccgg aagacgtcaa
    27181 cgccgaagcg gtttcgccag aagagtggca ggccgaagcg gaaaccgtag agattgtcga
    27241 agcggcggaa gaagaagcgg ctaaagaaga aatcaccgac gaagagccgg aggcacaggc
    27301 gctggctgcc gaagcggcag aagaggcggt aatggtggtt tctccggcag aagaagagca
    27361 gccggtggaa gaaatcgctc aggagcagga aaaaccgacc aaagaaggtt tcttcgcgcg
    27421 cctgaaacgc agcctgttaa aaaccaaaga aaatctcggt tccggattta tcagcctgtt
    27481 ccgcggtaaa aaaatcgacg atgatctgtt tgaagagctg gaagaacagc tgttgatcgc
    27541 cgatgtgggc gtggaaacca ctcgtaaaat tatcaccaat ctgacagaag gcgcatcccg
    27601 caagcagctt cgtgatgccg aggcgctcta tggcctgctg aaagaagaga tgggcgagat
    27661 tctggcgaaa gtcgatgagc cgctgaatgt tgaaggtaaa acgccgttcg tgatcctgat
    27721 ggtgggcgtc aacggtgtgg gtaaaaccac gacgattggt aagctggcgc gtcagtttga
    27781 gcagcagggt aaatcggtga tgctggcggc aggcgatact ttccgtgcgg cagcggttga
    27841 acagcttcag gtctggggcc agcgcaacaa tattccggtg attgcccagc ataccggtgc
    27901 ggattccgct tctgttatct tcgacgccat tcaggccgct aaagcgcgta acatcgacgt
    27961 cctgattgcc gataccgccg gacgcctgca aaacaaatcg cacctgatgg aagagttgaa
    28021 gaaaatcgtc cgcgtgatga agaaactcga cgttgaagct ccgcatgaag ttatgctgac
    28081 catcgatgcc agcaccgggc agaatgcggt aagccaggcc aaactgttcc acgaagccgt
    28141 tggcttaacc ggcatcacgc taacgaaact ggacggcacg gcgaaaggcg gggtaatttt
    28201 ctcggtggct gaccagtttg gtatccctat ccgctacatt ggtgtcggcg aacgtattga
    28261 ggatttgcgt ccgtttaagg cggacgactt tatagaggca ctttttgccc gagaggatta
    28321 acaatgattc gctttgaaca tgtcagcaag gcttatctcg gtgggagaca ggcgctgcag
    28381 ggcgttacgt tccatatgca gccgggtgag atggcgtttc tgaccggtca ttccggcgca
    28441 gggaaaagta ccctcctgaa gctgatctgt gggattgagc ggcccagcgc cgggaaaatc
    28501 tggtttagcg gccatgacat cacgcgtctg aaaaaccgtg aagttccgtt tctgcgccgc
    28561 cagattggca tgattttcca ggatcaccat ctactgatgg accgtactgt ctatgataac
    28621 gtggcgatcc cgctgattat cgccggtgcc agcggtgatg acattcgtcg ccgggtgtcg
    28681 gcggcgctgg ataaagtcgg gctactggac aaagcgaaga acttccctat tcagctttcc
    28741 ggcggtgaac aacagcgtgt tggcattgcc cgcgcggtgg tgaacaagcc cgcggtactg
    28801 ctggcggacg aaccgactgg taacctggac gacgcgctgt cggaaggcat tttacgtctg
    28861 tttgaagagt ttaaccgcgt tggagtaacc gtattgatgg caacgcacga catcaacctg
    28921 atttcgcgac gttcctatcg catgctcacc ctgagcgatg gtcacttgca tggaggcgtg
    28981 ggccatgaat aagcgcgatg caatcaatca cattcggcag tttggcgggc gtctggatcg
    29041 cttccgtaaa tcggtcggcg gctctggcga cggcggtcgt aacgcgccga aacgcgcgaa
    29101 atcctcgcca aaaccggtaa atcgcaaaac caacgttttc aacgaacagg tacgctatgc
    29161 cttccacggc gcattgcagg atctgaaaag caaaccgttc gccacgtttt taacggtgat
    29221 ggttatcgcc atctccctga cgctgcccag cgtctgttat atggtgtaca aaaacgttaa
    29281 ccaggcggcg acgcagtatt atccgtcacc gcaaatcacc gtttatctgc aaaaaacgct
    29341 ggacgatgac gccgctgcgg gcgtggtggc acagttgcag gccgagcaag gcgtagagaa
    29401 agtgaattat ctttctcgtg aagacgcact gggcgagttc cgtaactggt ctggttttgg
    29461 tggtgcgctg gatatgctgg aagaaaaccc gcttccggca gtagcggtgg taatcccgaa
    29521 actcgatttt caggggacgg aatcactgaa tacgctgcgt gatcgtatca cccagatcaa
    29581 cggcattgac gaagtgcgga tggatgacag ctggtttgcc cgtctggcgg cgttaaccgg
    29641 gctggtcggg cgcgtttcgg caatgatcgg cgtattgatg gtggcggcag tgttcctcgt
    29701 catcggtaat agtgtgcgcc tgagtatctt tgctcgccgt gattctatta acgtgcagaa
    29761 actgattggt gcgacggatg gtttcatcct gcgtccgttc ctgtacggtg gcgcattgct
    29821 gggattttct ggcgcattgt tgtcactaat tttgtcagaa attctggtgc tgcgtttgtc
    29881 atcggcggtt gcggaagtgg cacaggtttt cggaacgaag tttgatatca atggcttatc
    29941 gttcgatgaa tgcctgctat tgctgctggt atgctcgatg attggctggg tggcagcgtg
    30001 gcttgccacg gtacaacatt tacgccactt tacgcctgaa taataaaagc gtgttatact
    30061 ctttccctgc aatgggttcc gtagcaggga aagagacccc gttgtctctt cccggtattt
    30121 catctctatg tcacattttg tgcgtaattt attcacagcg ttgcattgaa cttgtggata
    30181 aaatcacggt ctgataaaac agtgaatgat aacctcgttg ctcttaagct ctggcacagt
    30241 tgttgctacc actgaagcgc cagaagatat cgattgagag gatttgaatg actgacaaaa
    30301 tgcaaagttt agctttagcc ccagttggca acctggattc ctacatccgg gcagctaacg
    30361 cgtggccgat gttgtcggct gacgaggagc gggcgctggc tgaaaagctg cattaccatg
    30421 gcgatctgga agcagctaaa acgctgatcc tgtctcacct gcggtttgtt gttcatattg
    30481 ctcgtaatta tgcgggctat ggcctgccac aggcggattt gattcaggaa ggtaacatcg
    30541 gcctgatgaa agcagtgcgc cgttttaacc cggaagtggg tgtgcgcctg gtctccttcg
    30601 ccgttcactg gatcaaagca gagatccacg aatacgttct gcgcaactgg cgtatcgtca
    30661 aagttgcgac caccaaagcg cagcgcaaac tgttcttcaa cctgcgtaaa accaagcagc
    30721 gtctgggttg gtttaatcag gatgaagtcg aaatggtggc ccgtgaactg ggcgtaacca
    30781 gcaaagacgt acgtgagatg gaatcgcgta tggcggcaca ggacatgacc tttgacctgt
    30841 cttccgacga cgattccgac agccagccga tggcaccggt gctctatctg caggataaat
    30901 catctaactt tgccgatggc attgaagatg ataactggga agagcaggcg gcaaaccgtc
    30961 tgaccgacgc gatgcaaggt ctggacgagc gtagccagga catcatccgc gcgcgctggc
    31021 tggacgaaga caacaagtcc acgttgcagg aactggctga ccgttacggc gtttccgctg
    31081 agcgtgtgcg ccagctggaa aagaacgcga tgaaaaaatt gcgtgctgcc attgaagcgt
    31141 aatttccgct attaagcaga gaaccctgga tgagagtccg gggtttttgt tttttgggcc
    31201 tctgtaataa tcaatttccc ctccggcaaa acgccaatcc ccactcagat tgttaataaa
    31261 ctgtcaaaat agctattcca atatcataaa aatcgggtat gttttagcag agtatgctgc
    31321 taaagcacgg gtagctacgt ataaaacgaa ataaagtgct gcacaacaac atcacaacac
    31381 acgtaataac cagaagagtg gggattctca ggatgaacat aaagggtaaa gcgttactgg
    31441 caggatgtat cgcgctggca ttcagcaata tggctctggc agaagatatt aaagtcgccg
    31501 tcgtaggcgc aatgtccggt ccggtggcgc agtacggtga ccaggagttt actggcgcag
    31561 agcaggcggt tgcggatatc aatgctaaag gtgttatcaa aggcaacaaa ctgcaaatcg
    31621 taaaatatga cgatgcctgt gacccgaaac aggcggttgc ggtggcgaac aaagtcgtta
    31681 acgacggcat taaatacgtg attggtcacc tctgctcttc atcaacgcag ccagcgtctg
    31741 acatctacga agacgaaggc attttaatga tcaccccagc ggcaaccgca ccagagctga
    31801 ccgcccgtgg ctatcagctg atcctgcgca ccaccggctt ggactccgac caggggccga
    31861 cggcggcgaa atatattctt gagaaagtga aaccgcaacg cattgctatc gttcatgaca
    31921 aacagcagta cggcgaaggt ctggcgcgtg cagtgcagga cggcctgaag aaaggcaatg
    31981 ctaacgtggt gttctttgat ggcatcaccg ccggggaaaa agatttctct accctggtgg
    32041 cgcgtctgaa gaaagagaat atcgacttcg tttactacgg tggttatcac ccggaaatgg
    32101 ggcaaatcct gcgtcaggca cgcgcggcag ggttgaaaac tcagtttatg gggccggaag
    32161 gtgtggctaa cgtttcgctg tctaacattg cgggcgaatc agcggaaggg ctgctggtga
    32221 ccaagccgaa gaactacgat caggttccgg cgaacaaacc cattgttgat gcgatcaaag
    32281 cgaaaaaaca ggacccaagc ggcgcgttcg tctggaccac ctacgccgcg ctgcaatctt
    32341 tgcaagcggg gctgaatcag tctgacgatc cggcggagat cgctaaatac ctgaaagcga
    32401 actcggttga tacggtgatg gggccgctta gctgggatga gaaaggcgat ctgaaaggct
    32461 ttgagttcgg cgtatttgac tggcacgcca acggcacggc gaccgatgcg aagtaatcat
    32521 taatcggcaa ctttgggttg ccgccaaatt gctaatatcg agtacgttgc ttcatgccgg
    32581 atgcggcgta aacgccttat ccggcctaca agattcaaag aaatcagtaa attgcagcac
    32641 acattgtagg cctgataagc gtagcgcatc aggcaatatc acttttgaaa tcaaacttga
    32701 cgattaacgc ttctcccagc caccctgttg tgccgtaaat cccagtgctc gcataaacgc
    32761 cgtcatcacg ccgcgatctt ccacgccagc atccgccatc caccaacatg aaacgtcagg
    32821 attgttacgc aaaacctctt ccagtagata ttgccccaca ccgcgacgtc gggtgacttc
    32881 ccgcacgcgt agagaatcca gtgccccctc ggtgccgctt aaggttaccc gcacggcagc
    32941 gagaaggcgc tcgttaaaac gcgcggcgta gatacggtgg ttatcgtcaa cctgtaaaga
    33001 ggaaggggaa tactccggcc agatcttttg caggtcaatc cggtcctggt cgctaaagtt
    33061 ttctaatcga atgatggtca gcttcatagg gaacccgtgt aaatcacaaa agtggaacca
    33121 gtgtagcgaa ataatttaat cggaggcttt ctctttttta attcttttgg caggtgatta
    33181 attttttaac
//
LOCUS       DAZKHK010000046        33120 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKHK010000046 DAZKHK010000000
VERSION     DAZKHK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.046"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..842
                     /gene="gatY"
                     /locus_tag="ACHOS5_002182"
     CDS             <1..842
                     /gene="gatY"
                     /locus_tag="ACHOS5_002182"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP9776998.1"
                     /translation="TKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAPVIIAG
                     TPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMIDASH
                     LPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQAREFAE
                     ATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTIK
                     LGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGCE
                     GRA"
     gene            871..2133
                     /gene="gatZ"
                     /locus_tag="ACHOS5_002183"
     CDS             871..2133
                     /gene="gatZ"
                     /locus_tag="ACHOS5_002183"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP9776999.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQVLAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSRCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIHYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2143..2595
                     /gene="gatA"
                     /locus_tag="ACHOS5_002184"
     CDS             2143..2595
                     /gene="gatA"
                     /locus_tag="ACHOS5_002184"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP9777000.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2626..2910
                     /gene="gatB"
                     /locus_tag="ACHOS5_002185"
     CDS             2626..2910
                     /gene="gatB"
                     /locus_tag="ACHOS5_002185"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9777001.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2914..4269
                     /locus_tag="ACHOS5_002186"
     CDS             2914..4269
                     /locus_tag="ACHOS5_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9777002.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYLGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIVIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            4317..5357
                     /gene="gatD"
                     /locus_tag="ACHOS5_002187"
     CDS             4317..5357
                     /gene="gatD"
                     /locus_tag="ACHOS5_002187"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9777003.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGLAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5463..6236
                     /locus_tag="ACHOS5_002188"
     CDS             5463..6236
                     /locus_tag="ACHOS5_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9777004.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6318..7217)
                     /gene="yegS"
                     /locus_tag="ACHOS5_002189"
     CDS             complement(6318..7217)
                     /gene="yegS"
                     /locus_tag="ACHOS5_002189"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP9777005.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            7633..7950
                     /gene="yegR"
                     /locus_tag="ACHOS5_002190"
     CDS             7633..7950
                     /gene="yegR"
                     /locus_tag="ACHOS5_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP9777006.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(8280..9641)
                     /gene="yegQ"
                     /locus_tag="ACHOS5_002191"
     CDS             complement(8280..9641)
                     /gene="yegQ"
                     /locus_tag="ACHOS5_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP9777007.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            10129..11343
                     /locus_tag="ACHOS5_002192"
     CDS             10129..11343
                     /locus_tag="ACHOS5_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HFP9777008.1"
                     /translation="MTGNIHQNYNARDVINAPEVKTAILQRSLFRQDNETIQKWLLNH
                     FYRWLISDFPMVQQINSLAEYSLFNKNDDVIPEWLVSKFNTASVSTATLYYIETDHQQ
                     ILTKERELVEFLSRKCGTRLESKLQRITCYVAFRMREEEHEKMLQRREKGWQPSEQNT
                     VKLILDVPDGKIVEFDASHSNLRREMAYESWHMQHCVGQFDDRKNLTGGYGEYYANQI
                     EQHKLRLFSLRDNNNIPHVTIALNVVGDSLEIDQIKGKQNRHPVKKYADDVLSLLQLL
                     SPQAVRHSDCEGMGIVYENTPEYQGWKYVTEVYETSFLLSVLHNNFHLLEHFTNPSVE
                     LQWLLLHSAPDKLHYLNAIDPIVATSAEMLFPGAEWHPQFAGQNISNISFEIESLTLQ
                     TSHYLPLSEVEK"
     gene            11343..12059
                     /locus_tag="ACHOS5_002193"
     CDS             11343..12059
                     /locus_tag="ACHOS5_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9777009.1"
                     /translation="MSLIIVLLVAIFIFIYRYKKNKPQIDAFIAKEKEKDLIKDKRRA
                     TKRRKWALAIGDIVARRNGLSVNGFTLDLKLTDDKRQQLALQVKQELGTETYQSNEDF
                     RQQIGVILQRWATGLGNSPHDFYEQLAAQGQVLDGLAFDCMRTAFLTRCIAGLGWCDE
                     NQAWIVLLLNAQRAQDCFASWEDYASAYVRARQKWLMIYDTPVATANRDLKEVTAWLK
                     DPSSNWKKLPWNEFKIFEPN"
     gene            12175..14177
                     /locus_tag="ACHOS5_002194"
                     /pseudo
     CDS             12175..14177
                     /locus_tag="ACHOS5_002194"
                     /inference="COORDINATES: protein motif:HMM:NF024624.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
     gene            14238..14843
                     /locus_tag="ACHOS5_002195"
     CDS             14238..14843
                     /locus_tag="ACHOS5_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777010.1"
                     /translation="MLMLKKGMMLVGLIFFSLPANAGCTLEETARKVIMGFQQEDAQL
                     INSLIDKKTGLYVLFHRGASMDVENLKQINFRQPVPEYFPWPSAGEHVPRDDEFNKKA
                     IPKFNCERGWNKQGYFISNSDVHHKVSRSLVFTQMFGSPQNTISDAQIAAARRMEYGA
                     VRVVAAPEESNDGLVFYLSQLYSYDSDWYLTVVDQIGDCGA"
     gene            complement(14968..15300)
                     /gene="yegP"
                     /locus_tag="ACHOS5_002196"
     CDS             complement(14968..15300)
                     /gene="yegP"
                     /locus_tag="ACHOS5_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP9777011.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            complement(15480..16202)
                     /gene="baeR"
                     /locus_tag="ACHOS5_002197"
     CDS             complement(15480..16202)
                     /gene="baeR"
                     /locus_tag="ACHOS5_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP9777012.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(16199..17602)
                     /gene="baeS"
                     /locus_tag="ACHOS5_002198"
     CDS             complement(16199..17602)
                     /gene="baeS"
                     /locus_tag="ACHOS5_002198"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP9777013.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPIDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            complement(17599..19014)
                     /gene="mdtD"
                     /locus_tag="ACHOS5_002199"
     CDS             complement(17599..19014)
                     /gene="mdtD"
                     /locus_tag="ACHOS5_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9777014.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGFSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDYLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSVQ"
     gene            complement(19015..22092)
                     /gene="mdtC"
                     /locus_tag="ACHOS5_002200"
     CDS             complement(19015..22092)
                     /gene="mdtC"
                     /locus_tag="ACHOS5_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP9777015.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVVLVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRVL
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            complement(22093..25215)
                     /gene="mdtB"
                     /locus_tag="ACHOS5_002201"
     CDS             complement(22093..25215)
                     /gene="mdtB"
                     /locus_tag="ACHOS5_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP9777016.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAITLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLSAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(25215..26462)
                     /gene="mdtA"
                     /locus_tag="ACHOS5_002202"
     CDS             complement(25215..26462)
                     /gene="mdtA"
                     /locus_tag="ACHOS5_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP9777017.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSVTPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKLVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     TQSATTPEEKATSREYAKKGARS"
     gene            26741..26797
                     /locus_tag="ACHOS5_002203"
     CDS             26741..26797
                     /locus_tag="ACHOS5_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9777018.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            27069..27125
                     /locus_tag="ACHOS5_002204"
     CDS             27069..27125
                     /locus_tag="ACHOS5_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9777019.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            27343..28002
                     /gene="yegL"
                     /locus_tag="ACHOS5_002205"
     CDS             27343..28002
                     /gene="yegL"
                     /locus_tag="ACHOS5_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9777020.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            27999..28760
                     /gene="pphC"
                     /locus_tag="ACHOS5_002206"
     CDS             27999..28760
                     /gene="pphC"
                     /locus_tag="ACHOS5_002206"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFP9777021.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALAMWLP"
     gene            28757..30697
                     /gene="yegI"
                     /locus_tag="ACHOS5_002207"
     CDS             28757..30697
                     /gene="yegI"
                     /locus_tag="ACHOS5_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP9777022.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALEMDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALVALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFLFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(30710..32062)
                     /gene="yegD"
                     /locus_tag="ACHOS5_002208"
     CDS             complement(30710..32062)
                     /gene="yegD"
                     /locus_tag="ACHOS5_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9777023.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAREKPDVIYLTGGSARSPLIKKALAEQLPGIPVAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            32196..33044
                     /gene="alkA"
                     /locus_tag="ACHOS5_002209"
     CDS             32196..33044
                     /gene="alkA"
                     /locus_tag="ACHOS5_002209"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP9777024.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LVVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
BASE COUNT         8266 a         8048 c         8597 g         8209 t
ORIGIN      
        1 cgacaaagca gatgctgaac aacgcgcagc gcggcggtta tgcggttccg gcattcaata
       61 ttcacaatct cgaaacgatg caagtggtgg tggaaaccgc tgccagcatg catgcgccgg
      121 tcatcatcgc cggaacgccc ggcacattta ctcatgccgg tacagaaaat ctgatggcgc
      181 tggtcagcgc gatggcgaag caatatcacc atccactggc aattcatctc gaccatcaca
      241 caaaatttga cgatatcgct cagaaggttc gttctggcgt gcgctcagtc atgattgacg
      301 cctcgcattt gccttttgcg caaaatattt cacgggtcaa agaggtggtg gatttttgcc
      361 atcgctttga tgtcagcgtc gaagcggagc tggggcaact tggcggccag gaagatgatg
      421 tgcaagtcaa tgaagccgat gctttttata ccaaccccgt tcaggcgcgt gaatttgccg
      481 aggcaaccgg gattgattcc ctggcggtcg ccatcggcac ggctcacggt atgtatgcca
      541 gagcaccggc gcttgatttt tctcgactgg agaacattcg ccagtgggtg aatttaccgc
      601 tggtgctgca tggcgcgtca ggattatcga ctaaggatat tcagcaaacc atcaaactgg
      661 ggatatgcaa aatcaacgtt gcaacagagc taaaaaatgc cttctcgcag gcgttaaaaa
      721 attacctgac cgagcacccc gaagcgaccg atccccgtga ttatttgcag tcggctaaat
      781 ctgcaatgcg cgatgtggtg agcaaagtga ttgccgattg tggctgcgag ggcagggcat
      841 aacgcacctg ccatttaaca aggaaaaaac atgaaaacgt taattgcccg gcataaagct
      901 ggtgaacata ttggcatatg ttcagtctgt tctgcacatc cgttggttat cgaagcggcg
      961 ctggcatttg atcgcaacag cacgcgtaaa gtgctgattg aagcaacgtc aaaccaggtc
     1021 aatcaatttg gcggttatac cggaatgaca ccggcagact ttcgcgaatt tgtttttgcg
     1081 attgccgata aagtcggatt tgctcgtgag cgtattattc tcggcggcga ccatctggga
     1141 ccaaactgct ggcagcaaga aaatgcggat gcggcgatgg aaaaatccgt cgagctggta
     1201 aaggcatatg ttcgtgccgg cttcagtaaa attcatcttg atgcgtcaat gtcctgcgct
     1261 gacgattcca taccgttagc gccagaaacg gttgctgaac gtgccgctgt gctttgtctg
     1321 gcggcggaaa gtgtggcgac tgattgccag cgtgagcaac tgaactatgt cattggcacc
     1381 gaagttccgg ttccgggcgg tgaggccagc gccattcagt cagtacacat cacccaggtt
     1441 gaagatgccg ccaatacttt acgcacgcat caaaaggcct ttattgcccg tggtctggca
     1501 gaggcgttaa cacgcgtgat tgccatcgtg gtgcagccgg gtgtggaatt tgaccatagc
     1561 aatattattc attatcaggc gcaagaagct caggtgttgg cgcaatggat agaaaaaacc
     1621 aaaatggttt atgaagcgca ttccaccgat taccagactc agacggccta tcgggaatta
     1681 gttcgcgatc attttgcaat tttgaaagtc ggtccggcat taacctttgc gttacgcgag
     1741 gcgatatttg cgctggcaca aattgagcag gaactcatcg ctccagaaaa tcgtagccgt
     1801 tgtctggcgg tgattgaaga agtgatgctc gacgaaccgc aatactggaa aaaatattat
     1861 cgcacgggtt ttaacgattc attgctggat attcattaca gcctgtcgga tcgtattcgt
     1921 tattactggc cgcatagccg gattaaaaat agcgtcgaaa cgatgatggt gaatctggaa
     1981 ggcgtggaca tcccactggg catgattagt cagtatcttc cgaaacaatt tgaacgcatt
     2041 cagtccgggg aattatcagc aataccgcat cagcttatta tggataaaat ttatgatgtt
     2101 ttgcgcgcct atcgctatgg ctgtgcggaa taaggacggt atatgactaa cctgtttgtt
     2161 cgtagcggaa tttcttttgt cgatcgtagc gaagttttaa cccatatcgg taatgagatg
     2221 ctcgcgaaag gtgtggttca tgatacctgg ccacaggcat taattgccag agaagcagaa
     2281 ttccctaccg ggataatgct tgagcagcac gccattgcaa taccgcattg tgaggcgatt
     2341 catgctaagt cgtcagccat ttatctgtta aggccaacaa ataaagttca ttttcagcaa
     2401 gcggatgatg ataacgacgt ggcggtatcg ttggttattg cgttgattgt ggaaaatccg
     2461 cagcagcaat tgaaactttt acgctgttta tttggcaagt tacaacagcc tgatatcgtc
     2521 gagacactaa tcactcttcc tgaaacccag ttaaaggaat acttcacaaa gtatgtttta
     2581 gattcagacg aataaatccc tctgtaacaa taaaaaggac tatttatgaa acgcaaaatt
     2641 attgtcgctt gcggaggcgc ggttgcgacc tctacgatgg cggcggaaga aattaaagag
     2701 ttgtgtcaga gtcataatat tcctgttgaa ttaatccagt gtcgggttaa tgaaatagaa
     2761 acctatatgg atggcgtgca tttgatatgc accactgcca gggtggatcg tagttttggc
     2821 gatattccgt tagttcacgg catgcctttt gtttctggtg tcggtatcga agcattacaa
     2881 aataaaattc tgactatctt acaggggtga cctatgtttt cagaagtcat gcgttatatt
     2941 ctcgacctcg gccctacggt gatgctgccg attgtcatca ttattttttc taaaatatta
     3001 ggcatgaagg caggcgattg ctttaaagcg ggtctgcata tcgggattgg ctttgttggc
     3061 attggccttg tgattggctt aatgctggat tccattggtc cggcggcgaa agcgatggcg
     3121 gaaaatttcg acctgaatct gcatgtggtc gatgtcggct ggccgggctc ttcacccatg
     3181 acctgggcgt cgcaaattgc gctggtggcg attccgattg cgattctggt taacgtggcg
     3241 atgttactga cccgtatgac gcgggtggta aatgttgata tctggaatat ctggcatatg
     3301 accttcaccg gcgcgttgct gcatctggca accggttcat ggatgatagg gatggcgggt
     3361 gtggttattc acgcggcgtt tgtttataaa ctcggcgact ggtttgcccg cgatacccga
     3421 aatttctttg agctggaagg cattgctatt ccgcacggta catcggcgta tctggggccg
     3481 atagcggtgc tggtggatgc tatcatcgag aaaatcccag gggttaaccg tattaaattt
     3541 agtgccgacg atatccagcg caaatttggt ccttttggcg agcccgtcac cgtgggtttt
     3601 gtgatgggac tgattatcgg catcctcgcg ggttacgatg tcaaaggtgt attgcagctg
     3661 gcggtaaaaa cggcggcagt tatgctgctg atgccacggg tgattaaacc catcatggat
     3721 ggtttaacgc ccatagctaa gcaggcccgt agtcgtttac aggcgaagtt cggcggtcag
     3781 gagttcctga ttggccttga tccggcgttg ctgctgggac atacggcggt ggtatcggca
     3841 agcctgattt ttattccact caccatttta attgctgttt gtgtgccggg taatcaggtg
     3901 ctgccgtttg gcgaccttgc caccatcggc ttctttgtgg cgatggcggt cgcggtgcat
     3961 cgtggaaatc tgttccgcac cttaatttcg ggtgtcatca ttatgagcat caccctgtgg
     4021 atcgcgacgc aaactattgg tttgcacacc caactggcgg ctaatgcggg cgcgttaaaa
     4081 gccgggggta tggtggcttc aatggatcag ggcggttctc ccattacctg gttactgatt
     4141 caggttttct ccccgcaaaa tattcccggt ttcattgtta tcggcgcaat ttatcttacc
     4201 ggtattttca tgacctggcg tagagcgcgt ggctttatta aacaagagaa agccgttctc
     4261 gcagaataat ttttacctga gggggaggtt atccccctta acaatcagga gtttttatga
     4321 aatcagtggt gaatgatact gatggtatcg tgcgcgtagc agaaagcgtc attcctgaaa
     4381 ttaaacatca ggatgaggtg cgggtaaaaa ttgccagctc gggcttatgt ggttccgatt
     4441 tacccaggat atttaaaaat ggtgcacatt attatccaat aacgttgggc catgaattta
     4501 gcggctatat tgatgcggtg ggatccggtg ttgatgattt acaccctggc gatgcggttg
     4561 cctgtgtgcc gttattaccc tgttttactt gtccagagtg tctgaaaggg ttttattccc
     4621 agtgcgcaaa atatgatttt attggctcgc ggcgtgatgg tggacttgct gaatatattg
     4681 tcgttaagcg aaaaaatgtc tttgctctac ccacggatat gcctattgag gatggggctt
     4741 ttattgagcc gattaccgtt ggcctgcatg cttttcattt agcgcaaggt tgtgagaata
     4801 aaaacgttat tattattggt gccggaacca ttggcctgct ggctattcag tgcgctgtcg
     4861 cgctgggagc aaagagtgtg acggctatcg acattagctc agaaaaactg gcactggcaa
     4921 aatctttcgg tgcgatgcaa acatttaaca gccgtgaaat gagcgcgccg caaatacagg
     4981 gcgttttacg cgagctgcgc tttaatcagc ttatcctcga gacggctggc gtcccgcaaa
     5041 ccgtcgaact ggcggtagag attgccggtc ctcatgccca actggcgctg gtgggcacgt
     5101 tgcatcagga tctgcattta acatctgcaa cgtttggcaa aatattgcgt aaagagctga
     5161 cggttatcgg cagttggatg aactactcca gcccttggcc ggggcaggag tgggaaacgg
     5221 cgagccggtt gctgacagaa cgtaagttaa gcctggagcc attaatcgct caccgtggaa
     5281 gctttgaaag cttcgcccag gcggtgcgtg acatcgctcg taatgccatg ccgggcaaag
     5341 tgttgctcat tccctgaaac cgcgggccag cgtgatgcag gcccggtatt gtgcaaaaca
     5401 gatcattcac caatggtccc ccttcgttta cactagccac aattgaatat gggtaaatga
     5461 cgatgaattc attcgagcga aggaataaga tcatccaatt agtgaatgaa cagggaaccg
     5521 tgcttgttca ggatctggcg ggagtatttg ctgcctcgga agcgacaatc cgtgccgatt
     5581 tgcgctttct cgaacaaaaa ggcgtggtta cgcgctttca tggcggtgcg gcgaaaataa
     5641 tgtccggtaa tagcgaaacc gagacccagg aagtcgggtt taaagagcga tttcagctcg
     5701 ccagcgcgcc gaaaaacaga atagcgcagg cggcagtcaa aatgatccac gaagggatga
     5761 ccgttattct cgacagcgga agtaccacaa tgcttatcgc cgaaggatta atgaccgcca
     5821 aaaatatcac ggtgattacc aacagtctcc cggcggcgtt tgccctttcc gaaaataaag
     5881 acattactct ggtcgtctgc ggtggcacgg tccgccataa aacgcgatct atgcatggat
     5941 ctattgctga acgttccctg caggatatta atgccgattt aatgtttgtc ggtgctgatg
     6001 gtattgatgc ggttaatggt attacgacct ttaatgaagg ttattccgtt agcggtgcga
     6061 tggtgacagc ggcgaataaa gtgattgccg ttctcgattc atcgaaattt aatcgtcgcg
     6121 gttttaatca ggtactgcca atcgaaaaaa ttgacattat tattaccgat gatgctgtgt
     6181 ctgaggtgga taaactggca ttgcagaaga cacgggtaaa attaattacg gtgtagtatt
     6241 gttctgtatg ccggataagg tgtttacacc gtatccggca ttacggattc tctctggttc
     6301 aacagagaat agtttgatta acgcaacaat ggacaatctg gtggtaatcg acaacgcaac
     6361 gccgccggaa gtatttcaat atggaagttt tgcccactca acggttcgcc atcaagatta
     6421 aaagtgattt cgtgtggggc ttgtatatca aaccacgacg aagctccttc gataatattc
     6481 gggttatctt cgtcagattt taaggttgat acgagagtcg gaataatttc atcgccggta
     6541 aaaatgcgca gttgcagcaa gccatcgtta attaatgcgt tcgggcacaa ttgttgaccg
     6601 ccaccggcct gacgaccgtt accaatacca atgaccaggg cgtcaccttg ccagtgaaag
     6661 ttttcaccgc ggatttcaca acggtccggt tgcagagtgt ccatgcgcat taagccatga
     6721 atgatgtaag agacgccacc cagcgcggct tttaattttt ccggcgtttc tgtggtaata
     6781 cgcgtcccaa acccgcctgt cgccatatta ataaaacagg tttgtttgtt gacctgcgcc
     6841 atatctatcg caatggcgtt accggcaatt gccagtttca gcgccttatc cagtgcctca
     6901 ggaatcccta cactggtggc aaaatcattg gcagttccta atggtaaaat tcccagcgcg
     6961 ggtatgtcat ccccctcaca ttgaatcaat gccgtagaaa cttcattaat ggtgccatcg
     7021 ccaccaccgg caatcactgt tgcgacacct aacttccggg cctcctctac aaatcgtgca
     7081 gcatcgcctt tctcccaggt gatccgcaca tggatcgtca ttccttcctc acgcaacagc
     7141 ataattgctt cgcgcaaggg tagattgtca gtacttttgc cattaagaat cagtaagctg
     7201 gcgggaaatt ctgccatgat cgtatgtgcc tttatgattg gtctgtagat agtgtagagc
     7261 agaaaagaaa aggtggagtc agaacaggat gaaagtcgga ggataaaaag tcagttcagg
     7321 tatggggatt cagtgcttac caccaggagc ttacaatgag gacagcgaat attaacggta
     7381 gccactgata aatttttaaa aaagagaata tacctaatat tcaactaaac agtggcatct
     7441 tcaatatact attcaaaata tattaaagtc ccccatgggg ttgcccggaa gggcctccat
     7501 gtctgtaatt cctacttatg taggaaatgc tgtacagaac atttattata atcctattca
     7561 attataataa tcatgccatt attatattta aacactagag aatgtcgttg gtaattaatg
     7621 ggggaaggta aggtgaaaaa gatagctgct atatcattaa ttagtgtttt tcttatgtct
     7681 ggttgtgctg tgcataatga tgagacaagt atcggtaaat ttggccttgc atataaaagt
     7741 aatattcagc gtaaactcga taaccaatac tacaccgaag ccgaagcttc tttagccagg
     7801 ggtagaatat ctggtgcaga aaatatagta aaaaatgatg caactcattt ctgtgttact
     7861 cagggcaaaa aaatgcagat agttgacctg aagacagaag gtgcaggatt acatggcgtc
     7921 gctcgtctga cattcaaatg tggagagtga gaatattttt tggtaagcgt caaacatgcg
     7981 cgttctggct gtgcgtaacc ggaacctgtg cgagcacgat gtcgataagt gaaaggcatc
     8041 gtgctatgaa ggaggattct atcgatgtgg tcaatggaag acggtgacca gggatagggc
     8101 ttatgcataa aaaataagcc cgtgtaaggg agattacaca ggctaaggag gtggttcctg
     8161 gtacagctag cattttatgg gttatgtttt tcagcgaaac ggatgataac cttaataaat
     8221 gcagctgtat gtgatcggtt tctaagaatt ttccatccgg gaaaaataat cgaaattaat
     8281 cacttaccgt gggggttacg cgtggtttcc ccggagaaat tacgcatcag cagcgcgtaa
     8341 tttagctcaa gatcctgcgg caccgggagc cacacagtat aaccatcgcc tggtgcgacc
     8401 ggcattgctt cacctttggc gttttccatg tgctcaaggg taaagttaat gttgccttgc
     8461 ggcgtcatca gctcaaggct gtcgccaacg gagaatttat ttttcaccgc taccgccgcg
     8521 agctccccct tgcgctcacc ggtaaactca ccaacaaact gctgacggtc agaaaccgaa
     8581 taaccgtatt cgtagttctg ataatcgtcg tgggtatgac gacgcaggaa accttcggta
     8641 tagccacgat gcgccagacc ttccagcgtt tccagcaggc tggtatcgaa cggtttgccc
     8701 gcagcggcgt catcgatagc tttacggtaa acctgcgcgg tgcgtgcgca atagtagaaa
     8761 gatttggtac ggccttcgat tttcagcgaa tgcacgccca ttttggtcag gcgttcaaca
     8821 tgggcgatgg cgcgcagatc tttcgagttc atgatgtaag tgccgtgctc atcttcaaac
     8881 gcggtcatat actcgcccgg acgctgggct tcttcgatca taaacacttt gtcggttggc
     8941 gcgccgatac ccagcgtcgg ctcaacattt tgcaccggaa tcggctcgta cttgtgtacg
     9001 atgttgccga cgtcatcttc tttcccttcc tggacgttat actcccagcg gcaggcgttg
     9061 gtgcaggtgc cctggttcgg gtcgcgcttg ttgatatagc cagagagcag gcagcgaccg
     9121 gagtaggcca tgcacagcgc gccgtgaacg aagatttcga tctccatatc cggcacctga
     9181 ttgcggatct cttcaatctc ttccagtgac agttcgcgag agaggatcac gcgggtcaga
     9241 cccatttgct gccagaattt caccgtcgcc cagtttacgg cgttagcctg taccgagagg
     9301 tggattggca tttccgggaa gtgctcacgc accagcataa tcagccctgg atcggacata
     9361 atcagcgcat ccggccccat ttccaccacc ggtttcaggt cacggataaa ggttttcagc
     9421 ttggcgttgt gcggtgcaat gttgaccacg acataaaact ttttccccag cgcgtgggct
     9481 tcattgatgc cgagctgaag attttcgtgg ttgaattcgt tgttgcgtac acgcagggag
     9541 taacgcggct ggcccgcata aacagcatct gcgccataag cgaaagcgta acgcatattt
     9601 ttcagcgttc ccgccgggga aaggagttcc ggtttaaaca taattctctc gttctgatga
     9661 caggtcagag ccgcttcacc tgatgaagcg gttaggggag tgcccccact ttaagggcgg
     9721 gcattgtagc gcaatcggcg ggtttagctc cagtgtaaat gtgaggggag aagtggcaga
     9781 gatgggagta tctacacgaa atcttcatgt agtgataaaa gaaaaagttg acgatactga
     9841 ctactttcct gaatgttcat cccctgataa ttatgaatgt atttgccgtc ctcttacagc
     9901 ctgaattatt gaggatgtaa aataatgtca tttatctcaa ttattcacta aaataaactg
     9961 caaatataag acataatttc caaagtttgt gacgctgcca ctattgagtt tacgttattt
    10021 tcttgagtgg cttacttgag tatgtacctg caaccatgct gatttagata aaaaatctat
    10081 agtcaacaat tattgctttg agtgatataa taaacacggg cggatgttat gactggaaat
    10141 attcaccaga attataatgc aagggatgta attaatgccc cggaagtaaa aacagcaatt
    10201 ttgcaaagaa gtctgtttcg ccaggataat gagaccatcc aaaaatggtt actcaaccat
    10261 ttttatcgat ggctaattag tgactttcca atggtgcaac aaattaactc gcttgcagaa
    10321 tattcactgt ttaataaaaa tgatgatgta atcccggaat ggctggtatc gaagtttaat
    10381 actgcctcag tatcaactgc gacactttat tatatcgaaa ccgatcatca acaaatatta
    10441 accaaagaac gggagttggt ggaatttctt tccaggaaat gtggtacccg tctggaatca
    10501 aaactacagc gcataacctg ctacgttgcg ttcagaatgc gtgaagaaga gcatgaaaag
    10561 atgcttcaac gacgcgagaa agggtggcag ccgagtgaac aaaatactgt aaagttaata
    10621 cttgatgtgc cagacggaaa aattgttgag tttgatgcca gtcattcgaa ccttcgtcgt
    10681 gaaatggctt atgaatcatg gcatatgcaa cattgcgtag gccagtttga tgatcgaaaa
    10741 aatcttaccg gtggttatgg tgagtattat gcaaatcaaa ttgagcaaca taaattacgg
    10801 ttattcagtt tgcgtgataa taataatatt cctcatgtta ctattgctct caatgttgtt
    10861 ggtgattcac ttgaaattga tcaaataaaa ggtaagcaaa atcgtcatcc agttaaaaaa
    10921 tatgcggacg atgttttgtc tcttttgcaa ctattatcac cacaagcagt tcgacatagt
    10981 gattgtgaag gtatgggtat tgtttatgaa aatacacctg aatatcaggg gtggaaatat
    11041 gtaacggaag tatacgaaac cagtttttta ctaagcgtac tacataataa ctttcattta
    11101 ttagaacatt ttacaaatcc ttcagtggag ttgcagtggt tactgttaca cagcgcacct
    11161 gataagttac attatcttaa tgctattgac cctatagttg ctacttctgc ggaaatgtta
    11221 ttccctggcg ctgagtggca tccgcaattt gcaggtcaaa atatcagcaa catttccttt
    11281 gagatagaga gtttgacatt acagacatct cattacttac ccctgtcaga ggttgaaaaa
    11341 taatgagtct tatcattgtt ctcctggtcg caatatttat ttttatctat agatacaaaa
    11401 aaaacaaacc acaaatcgat gcttttattg ctaaagaaaa agaaaaggat ctaatcaagg
    11461 acaaaagacg agcaacaaag agacgtaagt gggcattagc aattggtgat atagttgctc
    11521 gtcgtaatgg actgtcagta aatggcttca cgttagattt aaagttgact gatgataagc
    11581 gtcaacaact tgcactacag gttaaacaag agctaggaac agaaacttac caaagcaacg
    11641 aagatttccg tcaacaaatt ggcgttatat tacagcgctg ggctacaggg cttggtaatt
    11701 ctccacatga tttttacgaa caactggcag cacaaggcca ggttctggat gggctggcgt
    11761 ttgactgtat gcgcactgct tttttaactc gttgcatcgc cggtttaggc tggtgcgatg
    11821 aaaaccaggc ctggatagtg ttattactga atgctcaaag agcacaagat tgtttcgctt
    11881 catgggaaga ttatgccagt gcttatgttc gcgcccgaca aaaatggtta atgatatacg
    11941 atacccccgt cgccactgcg aaccgtgatt taaaggaagt cactgcatgg ttgaaagatc
    12001 catccagtaa ctggaaaaaa ttgccgtgga atgaatttaa aatttttgaa cctaattaaa
    12061 aggatatcta tggaacagga tgctcgttta ctcagtgcaa tgcaaaagat gtgtgaccaa
    12121 aaaatgccac ttaatactct tgagagaaaa tggcaaatcg caaatattcc ttatatgtta
    12181 caagaaaaac gttatcagga actagatgaa atttataacc aggcattaca ggagagtttc
    12241 acttctcgtc aggctgaaaa gcgctatttt ttatcctgga cccaaatgtg caactatttt
    12301 tatgatatga acacgctagt tgatgccgga acagaaggtt taagactcat taaaacctgg
    12361 cagcaagcaa gacctcattc tacacatgcc tggcttgccg aagcacaata ctggaatcac
    12421 cgtgcatggt tatatcgcag ttatggctgg gcaaatgata ccacacatgc aatgtggctt
    12481 tgtgctggtg catgtaatga acaaatggtc atcgctacac ttaaagcaat tgattgtgac
    12541 ccacgacaat ggatggctgc attacttacc agcaccaact caaaagtatt tggtcagcca
    12601 gcctggctgg cggcacacct taacggtgat agtgtcgcag gaataccatt aatgatcgca
    12661 ctgaaaaatt atcaccgccg ctccccgcaa gaagtgaagc gttgatggcg tattccggac
    12721 tttcgtttga gcatgcaata tgccccgttt tgcctcgccc caatatactt cctgagtatg
    12781 atgatgatgg tgggcaaaaa tattggcttt ctgtttgcct gactattttt cctcacactt
    12841 tttatccttt tgttgaatat ataccatttc gaatgccacg ctggggaggg tcgcataaag
    12901 aaatttccga attgctggat tcagtaacct gtaagcactt atctaccgaa gaacatgact
    12961 atatggatct cttactttgg tgggatgatt accgagatgt cagcattgag gacattgcgc
    13021 ccgaggagca acaatatgca atcgacttag cagagaatat tgctcagcac gcacaatttc
    13081 aggaatgtcg ccataatgcc ctagagtggt tacttgcctg ctataacaag cagaacgatc
    13141 acgataagtt gtggtgctgt atacagcgag cggtgatgga agacatgaag ctcaataatt
    13201 attacactgc gtacgcgata aaatttgcat taagttatta tccagattca ttctggatat
    13261 ataattttgt ctgccaaaat tcacagaata caacttatgc tacacctgtt atttataggg
    13321 ggtttttcca gcgagagggt attttgggtt ttgaaaaaga tgaagggcag ggcgatgctt
    13381 ggctggaaaa agctagtgac attaaataca atcataattg gcggtcggca ataaaagatt
    13441 tgagttggtt tgacctttct gattatttca ttccgcttgc gacaattggt aagcaaagaa
    13501 atattcctgc cgcattaaat ttagttgctc ttgaatattt ggataaggaa gacaatacaa
    13561 aattacctta tgaaccatct acagctttgg agtattttcg acgggcactt aaaatacttc
    13621 aagatgacct aaatttccat agctcggtat cttatccact ggtaaaaaat tatggttata
    13681 gcgaacatca acaagatcta caaaatattt attttagcat tgctatctgc taccaggcac
    13741 taaataaaca agaaatttct aaagaaacac gggctattta tgaaaaaaat ctccttgaca
    13801 atttatttct ggctcatgaa gctggacatg aaaaagcatg ggggcttttt ttacttaata
    13861 tctttgaggt gaaagaactc tcacttgctc atttgcacct ccaacaggta caagaagaag
    13921 cgaataaagg tacgctcgaa gcaatgatta ctttgtcgcg cctgtatggt aacaaagaag
    13981 atgaaaaact gtttaacatg aaactcagtg cgcgctggac acattttgcc gagtctttat
    14041 atccagataa cgaaattatt gcagattgcc tttatcatct ccatttctcc agcctgtgga
    14101 aacgctgtcg ttatgcctgg tatacctttc gtattccagc tagcgaatta cccggccagg
    14161 ttaacagtat ggtgtaaggt ttatcctgaa aattgttaaa tgtgtaatat gagccccatg
    14221 atgagaagat gcaaacaatg ctgatgttaa aaaaaggaat gatgctggtg gggctgatat
    14281 tcttttcttt acccgccaat gccgggtgta cgctggagga aacagccaga aaggtgatta
    14341 tgggctttca gcaagaagat gcccaattaa tcaactcgtt aattgataaa aaaacgggtt
    14401 tgtatgttct tttccaccgt ggtgcttcga tggatgttga aaatcttaag caaattaatt
    14461 tcaggcaacc tgttcccgag tatttcccat ggccctcggc aggggaacat gttccgcgag
    14521 atgacgaatt taataagaaa gctatcccga aatttaactg cgaacgtggc tggaataagc
    14581 aggggtattt tattagtaat agcgatgttc accataaagt cagccgtagt ttggtcttca
    14641 cgcagatgtt tggttcaccg caaaatacta tttctgatgc gcagattgcc gcagcgcgtc
    14701 gaatggaata cggggccgtg cgtgtggtcg ctgcgccaga agagagtaac gatggactgg
    14761 tattttattt aagtcaactt tacagttatg acagtgactg gtatctgacg gtggtggatc
    14821 agattggtga ttgtggggcg tgataaattc tgatactgaa acttatttac tcattatttt
    14881 aatgacggag gttaatctta attatcaata aataagcgtt attttgatat aaagccggac
    14941 gccaacaacc tacgcccggc atcgtaatta cgtattgtct ttcaccgtct ggcttgtgcc
    15001 atttacttta acggaggcaa ttccggtttc acgagattgc gcggtggcat acatctggct
    15061 ggagccgata atttgatgat tggcggcttt cagattgaaa tagaatttgc cattgcttgc
    15121 cgtttttttc tcatagcgtt cttcttgcgg gctgttgcta cgcaccgacg cgatgccctt
    15181 ttccgcagag gcttttgagg tatacagctc gctggtgagg atagtctcac cattgcccgc
    15241 ttttagcaca aaccggaact gattatcact gctcttactg agttcaaacc aaccagccat
    15301 aatgactcct tgattgaaat tgcgtatcga aaacagcatc taaaggtact gtttgccaat
    15361 taaagtatga gcgattttct ggtgcctgcc agggcggaga gataatgtca gtttgctgac
    15421 catgcgtggg ataatgttgc cggatctggt ctgaaggcca tctccggcag gtaaaaatgt
    15481 taaacgatgc ggcaggcgtc ggcttcccag cggtaaccga cgccgtaaac ggcgcggata
    15541 aatgactgtt cggcgtcgag agattccagc ttgcggcgca ggtttttaat gtggctgtcg
    15601 atggtacggt cggttaccac gcggtagtcg tcataaagat ggttcagcaa ctgctcgcgg
    15661 gagaacactt ttcctggttc atgagagagc gttttcagca gacgaaattc tgcaggcgtc
    15721 aggtcaagca ttttaccgcg ccaggaagcc tgaaaacgac cttcgtcgat aatcaacggg
    15781 ctttcagcat cctgttgctg caactcacgc tgcggtttgc aacggcgcaa aatggttttg
    15841 acgcgcgcta ccacttcccg tgggctgtac ggcttgcaga tatagtcatc tgcgccaatc
    15901 tccaacccca gcaggcggtc gatctcttcg atttttgccg tcaccatcac gatcggaatg
    15961 tcagaaaaac gacgaatttc ccggcacagc gtcaggccat cggtgccagg gagcatcaga
    16021 tccaacagga tcagatccgg tggtgtctgg cgcacatatg gcagtacctg atcgccgtgg
    16081 ctaatcaacg ttggcgcata gctcgcagca cgcagataat caatgagcaa ctgccccagc
    16141 ttcggttcat cttccacgat caaaatacgc ggtgtgtttt cgtcgattgg taactcggtc
    16201 atacttctct ctgtaaatcc cgttccagcg gtaactctac tgtaatgcta agcccgccaa
    16261 aaggcgaatg ggcggcaata atgcggccgt tatgtgcttc aacaatgttc aggcaaatcg
    16321 ccagccccag cccggaacca ccgctggcgc ggttgcggga accttcggtg cgataaaaac
    16381 gttcaaacaa tttttgtagc tgatcgtcac tgacacccgg cgcactgtcg gcaaaggtca
    16441 ggcgcaccgt tttgtcgtgc tgctcggcag agattttcag gctgccgccg ctgtcagtgt
    16501 agcgcaggct gttttccagt aagttattga acaactgcat taaacgatcg cgatcgccaa
    16561 atacggtaat actgtctggc agggaaaatt gcaatttcag gccacggctg gcgaatcgtt
    16621 cgcgaaatgc gcctcccgcc acttccagca gtgggatcaa atctatcggt gctttttgat
    16681 aggcgagagc gccttcatca gacatcgaca actgatggag atcgtcaacc agttttgtca
    16741 gtgttccgac ttctgcctgt aaagaagcca gcgtctccgg cgtgaattta cgcacaccat
    16801 cctgaatggc ttccagttcg ccgcgcagta ctgctaaagg cgtgcgcagt tcatgagaaa
    16861 tatcggccat aaaatcgcgg cgcatctgct ggtttttctc cagtgtgctg gcgagttgat
    16921 tgaaatcctc tgccagtctg cccaactcat cttcgctggt gggggctacg cgagtagtga
    16981 aatcacccgc tgccagtttg tgcgtgccat ccaccagtcg ttttaccgga gccagcaaac
    17041 cgcgcgccag tgggaatgtc gccagcgccg ctaataaggt agataaagcc acaatcagcc
    17101 aactggtttg ccgctgttgt ctgtcaaaat tgatatcagt attacgggtc agtcgttcaa
    17161 caggggaggc gatcaccgcg ccaacttccg caccattgac cataatgggc cgccgtgtgc
    17221 cgtcgggtgg aaccggtgct cgcgggccaa ccagcacttt attattttga tcaaccaccc
    17281 aaaattgcgt gcgccagccg tgtggtggca taccggggcc gggcttatct tcgttgttat
    17341 catgctccag tgaacgtagg atctgaaata caaagcgatc gttattacgc aggaagcgcc
    17401 agttcccgtg ctgggcgtac tgctcaccaa gcgcatcgcc gagcatttgc agccgttgtt
    17461 cattaccatg cttgatgtaa tcgataaagc cgcgctcaaa actgatacgt accgcccagt
    17521 gcatcgtgat caataagaca atgcaggtag cgaaaatcgc cagaaacagt ttgccggtaa
    17581 taccgggtcg ccagaacttc attgcacgct ccttttccgc cgtgaaatag ctacattttg
    17641 atgcgtatcg ctcggcactc tggcaaagac aaacgccgga agggcgatga taaacgccat
    17701 gctaagccag gtgtacataa agacggtttg tgtggtgccg ctgtcgacgc tgacatgctg
    17761 cgaaccaaaa agtcccagca acagcccggc gatggtgacg ccaatactca tcgacaattg
    17821 cataatcatc gacagcagac tgttgccgct gctcgccagg tagtccggga gatctttcag
    17881 cgtcagggtg ttcatggagg agaaacgcgt cgagttgacc attccttgta aaaacaggac
    17941 gaacggcaaa acgtagtacc agcccagcag agcggtagtc ataaacaaca gggtgaccag
    18001 cgacaggccc agcgtggtcg ccaccagtac ccgacgataa ccaaagcgat tcactacctg
    18061 taccacaatt cgcttcattc ccatgctgcc gagtaccatc gggatcatca tcagtccggc
    18121 atgaaacggc gagaaaccga ggccaatttg caggaaaacc ggtgtcataa agggcaacat
    18181 gccactgcca atacgtccgg caaagctccc cgccaggccc agcgaaaagg tacgagtgcg
    18241 gaacagtttc agactgaaca gggcgcggtt gttattttgg gcgtgcagca gataaagcac
    18301 cagtgctacc acgccaactg cgaccaggcc agcaatcgcc agcggcgaga aacctgtacc
    18361 tttactgccg tccagcgcca gggttaagac cgccatgcca accgccagca acagaaagcc
    18421 ggagagatca aagcgccgcg tctgcatggt gtagttcggc attaacatca gtgtggtgat
    18481 cgcgccaata atccccaccg gaatgttgat caaaaagatc cagtgccacg atgcgtactc
    18541 caccagcaga ccgccgagcg ccgggccaag cagcggcccg acctgaccgg gcagcgtgac
    18601 aaaggtcatc gccgccatat attgctcgcg cggtacgatt ttcatcaccg tcaacctgcc
    18661 gaccggcacc atcatcgcgc cgccaacgcc ctgtaacgcg cgtgccagca acagttcgtt
    18721 cagcgtgccg gaaagcgcgc aaaacagtga accgagagta aacagcacga tggcggtaaa
    18781 gaaaatattg cgcacgccga ctttgtccgc cagccagccg ctggcgggca gcatcaccgc
    18841 cacggtcagt acataagaga caatgaccat gtgcatatgc aacggacttt ccccgaggct
    18901 ttgcgccatt gagggaagcg cggtgttaac gatggtggtg tccagcgact gcataaagaa
    18961 gccgaaagcc acaatccaca attgccagcg ggtgctgtcg ggaagatctg tcatttactc
    19021 ggttaccgct tgtttaggtt tacgcgaaaa acgcagccgc agacggtcga aaaagagata
    19081 caccaccggc gtggtataca gcgtcagaag ctggctcatg accagaccgc cgacaatggt
    19141 gatccccaat ggttggcgca actccgagcc gtcaccgccc gacaatacca gcggtagcgc
    19201 accaaacagc gccgccaggg tagtcatcat aatcgggcga aaacgcagca gacaggcctg
    19261 gaaaatagct tcctgcggcg tcaggttacc gtgccgttgg gcttcaagcg caaaatcgac
    19321 catcataatg gcgtttttct tcacgatgcc gattaataac atgatcccta tcagcgcaat
    19381 taggctgaac ggggcattga acagctccag cgccaacagc gctccgacgc ccgccgaggg
    19441 tagggtggag agaatcgtca gtggatgcac ataactctcg taaaggatac ccagtacgat
    19501 atataccgtg gcgatggcgg cgataatcag gatcacctgt gagttcatcg tctcctggaa
    19561 cacttgcgct gttccggcaa aactgccgcg caccgtcgaa ggcacaccaa gctgggtcat
    19621 tgcgcgatcg atcgccgcac tggcgtccga gagcgatttc ccggtcggca ggttgaacga
    19681 aatggtcgag gccgccgata atccctgatg attcaccgac agtggagcgt tcgccggttg
    19741 ccatttagcg aaatacgaca gcgggatcgc ttttccttcg ttattgataa cgaacatttt
    19801 ttccagtgca ctgatgtcct gggtatagcg cggatcaact tccatcacca ctttgtactg
    19861 gttcatcggc tggtaaatgg tcgagatttg ccgctgaccg aaggcgttat ttaacaggct
    19921 gttggcggct tgcacgtcaa ttcccaatcg cgccatggtg tcgcgatcgt aaataaggtt
    19981 catctccgcg ccattatctt cctgatcgga gttaacatca gccaactctg gcaatgtcgc
    20041 cagttttttg cggattttcg gctcccattc gcgcagtgcc gccaggtcgt cggataacaa
    20101 cgtgtactgg taactggcgt tagcctgacg tccgccaacg cgaatatcct gtaccgccat
    20161 caggaacaga ttcgctcccg gttctttcgc cagtttttta cgcaagcgat cgataatctg
    20221 ttgggccgtt tcgctccgct cgccgcgtgg tttaagggtg ataaacatca ttccactgtt
    20281 cacccgtgaa ccgccggtaa agccggtgac attatccacc gccggatcgt cacggataat
    20341 tttcatgaag tcctgcaact taccgcgcat cgcctgaaac gaaatactct ggtcagcctg
    20401 tatcccgccc atcagtacgc cggtgtcctg ctccgggaag aaggttttcg ggatcgagat
    20461 atacagccag atattcagcg caatggtgcc aagcagcacc acgcccacca gacgggtatg
    20521 attgagcacc catttcagcg acttgccata gccttgttgc agggctacca acacgcgacc
    20581 aaagccacgc agtcgctttt gctcgcgcgg cttgctggct tttagcatcc agccgcacat
    20641 catcggcgtt aacgtcaggg aaaccagcaa cgaaatacca atggcgacag aaagcgtcac
    20701 ggcaaactcg cgtaacaggc gacccggcaa tccgcccatc aacagcagcg gcaggaacac
    20761 cgccaccagt gacagactca tcgacagcac cgtaaagccg acttcgcgag taccttgtag
    20821 cgcggcctgt agcggtttca tccccgcttc cagatgacgg gcaatgtttt ccagcaccac
    20881 gatggcgtca tccaccacaa agccagtggc gatggtgagc gccattaacg aaagattatt
    20941 gagactgaaa ccacacaggt acatcgccgc aaacgtacca atcagcgaaa ccggcaccgc
    21001 aacggcggga ataatggtgg cgcgacctga acgcaggaac aaaaacacca ccagaatcac
    21061 cagtacaact gagattacca gcgtctgctc aacctcttcc agcgacgcgc ggatagtagg
    21121 ggagcgatcc tgggcaattt gcagatcaat tgctgccggg atggtggatt gtaattccgg
    21181 cagacgggcg cggatgctgt ctaccgtctg gataatattg gcttccggca gcttgcggat
    21241 catcagcaaa atagccggtt tggcgttggt catcccggcg ttacgtacat cttgtacaga
    21301 gtcggtcacc gtcgccacat cgcccagacg aaccgcgccg ccgttgttgt aatgaataat
    21361 cagcggctga tattcagcgg cggttttcag ttcatcgtta gtctggatct gccagcggtg
    21421 agtaccatct tccagcgccc cctgcggttt acgcacgttg gcattgctga tggcagtgcg
    21481 tacgtcgtcc agcgagacgc cctgattaaa cagcgcctgc ggattcagcc cgacgcgtac
    21541 ggcgggcagc gagctgccgc caacatcgac atcaccgaca ccgtcgattt gcgaaatcgt
    21601 cggagccagc tgcgtcgagg cgaaatcgta cagttcaccc tgcgaataag tatcggatgt
    21661 cagcgtgagg atcataattg gcgcatccga cgggttcgct ttgcgatagg tcggacggct
    21721 gggcatcccg ctgggtagca gactttgcgc ggcgttgatc gccgcctgca catcgcgcgc
    21781 tgcgccgttg atatcccggt caaaatcaaa ctgcaaaata atgcgcgtgc tgccgagcga
    21841 actgctggag gtcatttcgc tgactccggc aatgcgccca agtgagcgct ccagcggcgt
    21901 ggcaacggaa gacgccattg tttctggtga cgcaccgggc agcgaggcgc tgaccatgat
    21961 caccggaaaa tcgacctgcg gcagcggggc gactggcagc atacggaaac ccagtatgcc
    22021 gcacagggta atggcgaccg acagtaaaat cgtcgccacc gggcggtaaa tgaagagggc
    22081 aaaaaacttc acttacgcct cctcttcatg acgggcaaag cggcttttgg tccacaatgc
    22141 cagacggtca aacagcagat aaatcaccgg cgtggtaaac agcgtcagca cctggctgac
    22201 aatcagaccg ccgaccatgc cgatacctaa cggacgacgc agttccgcgc cgaccccggt
    22261 actcaacatc agcggcagcg caccaagcag agccgccaga gtggtcatca ggatcggacg
    22321 aaaacgcaac agacaagcct ggtaaatagc ttcgcgcggc gacatgcctt gctcgcgctc
    22381 agcagccagc gcgaagtcga tcatcatgat ggcgttcttc ttcacgatac cgatcagcaa
    22441 aataatgccg ataatggcaa tcacatccag ttcgctaccg gcaatcatta acgccagcag
    22501 cgcgccgacc cctgcggtgg gtagcgtcga gagaatggtg atcgggtgaa taaagctctc
    22561 gtacagaatg ccgagcacga tatacatcgc caccaccgcc gcgacaatca gccagacagt
    22621 gctgcccaat gccgactgga aggcgagggt gctgccctgg aactgcgtgg tgatatccac
    22681 cggcagattc agcgtcttct ctgtgtccat aatcgcctgc accgcatcgc ccagcgaata
    22741 gttatccggc acgttaaagg agatagtcgt taccgggaac tgatccagat ggttgatgga
    22801 aagcggcgca aaacgctgct caattttggc aattgagctt aacggcacca cgccaccgtc
    22861 gctgctggtc aggcgaatgg tatccagcgc cgcgaggcct ggggtatttt cggtgttgtg
    22921 ttccagcacc acgcgatact ggttagcctg agtataaata gtggaaatca gtcgctgacc
    22981 aaacgcgttg tacagggcgt tatcgacatc cgccatgctg atccccaggc ggctggcgct
    23041 gtccctgtcg acattgacat acgccaccag ccctttgtcc tgccagtcgc tggagacatc
    23101 agaaagctgt ggcagctgct ggagtttttc catcaactgt ggcacccaag tactgagcgc
    23161 atccagtgac gtggcctgca aggtaaactg gtactgggtg cggctgacct gagtatcaat
    23221 agtcaggtcc tgcgttggtt gcaggaagag atcgacgccc ggcactttat ctaccgccgt
    23281 ttgcagacgg gcgatgactt tttgcacccg gtcatctcgt tcatccagtg gtttgagatt
    23341 gatctgtaaa cgcgcactgt tcagcgatgg gttagtgcca tcaacgccaa caaatgaggt
    23401 caaactttgc actgccggat cctgcaaaat cacgtccgcg acctggcgtt gccgctgggc
    23461 catattggca aaggagctgg attgcggtgc ctgcaaagtg ccctgaataa tgccgttgtc
    23521 ctgcaccggg aagaaacctt tcggaatgaa cacccacagc agcacgctaa gcagcagcgt
    23581 gctgagcgcc acgcttaagg tcagccacgg atgattcagc actttcgcca gtccacgacc
    23641 ataggcggcg attatcctgt cgaacatttt ctccgaggca cgggagaagc ggttctgttt
    23701 acgcagcgac tcctggctga gcatccgcgc gcacatcatc ggtgtcaggg tcagcgacac
    23761 caccgccgag atcaaaatcg cgactgccag ggtaatagca aattcgcgga acagtcgccc
    23821 gacgatatcg cccataaaca gcagggggat caacaccgca atcagcgaga aggtcagcga
    23881 gataatggta aagccgattt cacccgcgcc tttgagcgcc gccgccaacg gtttttcgcc
    23941 tttttcgata tagcgggaaa tattttcgat caccacgatg gcgtcatcga ccacgaatcc
    24001 ggtggcgata gttaacgcca tcaacgtcag gttattgatt gaaaaatcga gaaacaccat
    24061 gaccgcaaag gtgccaatta acgacagcgg tacagcaaca ccgggaatga tggtcgccgg
    24121 aatattgcgc aaaaacaggt agataatcat gactaccagc gcgatagcca tcatcagctc
    24181 aaactgggta tcgtcgaccg atgcgcggat attggtggta cggtcagaaa gtaccgtcac
    24241 cttcaccgat ttcggcagac tctcggtgag ctgtggtagc atctgccgga tgctgtcggc
    24301 ggtggagata atgttagcac cgggctggcg ctggacattc atcacaatgg cctgttcttt
    24361 gttagcccat gcgctgagcc agctgttttc tgctccttgc tctacagtag cgacatcgcc
    24421 cagacgaatt ggtgcgccgt tctggtaggc gatgattagc tggcgatact cttcggcgga
    24481 ttgcatctgg tcgttagcgg aaagcgtgat cgcgcgggaa gggccgtcga ggctaccttt
    24541 tgccgagtta acgttagcgc cggtaatagc ggtgcgtacg gtttcgctgg tcaggccgag
    24601 ggcggcaatc gcctgagcgt taagtttgac gcgaacagcc ggacgctggc cgccggaaag
    24661 cgtgaccagc cctacgccgg aaatctgcga gattttctgc gcgacgcggg tttccaccat
    24721 atcttccacc tgcgtcatcg gcatggcggt tgaggtgaca gcgagcgtca tgatcggtgg
    24781 atctgccggg ttcactttgc tgtaaaccgg cgggttaggc agatcgctcg gtagcaggtt
    24841 agtggcagcg ttaatcgcgg cctgcacttc ctgctcggcg acatccagcg gtaaggttag
    24901 ctggaactgc aaggtgataa ctgacgcgcc gccggaactt tgcgacgaca tttgtttcag
    24961 gccggacatc tgcccgaact ggcgttcgag cggcgcggta acggcagagg tcatgacatc
    25021 cgggctcgca cctggataaa gcgtgaccac ctgaatggtt ggatagtcca cttccggcag
    25081 cgccgaaacg ggcagggcgc gataaccgat aatcccggcg agtaagatcg ccaccatcag
    25141 cagcgtggtg gcgacaggac gcataataaa caggcgcgac gggccgcctg tgctgctcgg
    25201 aggtaacacc tgcatcagga gcgtgctcct tttttcgcgt attcgcggct ggtggctttc
    25261 tcttccggag tagtggcgct ctgggtttcc accacttcca ctttcgcccc ttcggtcagg
    25321 cgatcaattc cgtccgtcac tacgcggtcg cccgcagaaa tacctgcgcg gatcaccact
    25381 ttctgactgt cctgaatgcc cggtgtcacc agatgtttgc tgaccttgtt ttcgctattc
    25441 agcacccaga caaaatggcc ttcgttgccc atttgcagtg cggcggtggg gatcaccacg
    25501 gcgttttgtt cggtatcgac taacatgcgc gcgttaacaa actgattagg gaaaagcgca
    25561 tcatcctgat tattaaagcg tgctttcact ttaatcgtac cggtagtggc atcgatttgg
    25621 ttatcgagac ttaacagcgt gccttcactt aacttcttag agttggtgcg atcccaggct
    25681 tctaccacca gcggttttcc ggctttttgt gcctgtacaa cggtggcgat atcgctttcc
    25741 ggcagagtaa agaccagatc gataggatgt gtttgggtga tcaccacaat cccggtggtg
    25801 tcgccactgg agatttggtt accaacatca accagcttga gaccaacgcg accatcaact
    25861 ggtgcggtga tacggctcca gtcgagttgc aattgcgcgc tggcgacgct tgcttcatca
    25921 gccttaatgg taccttcggt ttcactgacc agcgcttgtt gggcatccag ttcctgacga
    25981 gatacgagat tggtttttgc cagttgttga taacgcgcca ggtcacggcg ggcgttggcg
    26041 agcgtggctt tatcttttgc cagttggccc tgggcttgtg ctaatgcaac tttgaactgg
    26101 cttgggtcaa tttctgccag taaatcgcct gctttgacct gctggccttc ctggaaatgt
    26161 aacgccatca gttgaccgtc cacgcggctg cgcaccgtaa cagtattagc ggcggtaatg
    26221 gtgccaagcc cggtgaggta acgcggaaca gcctgttcta ctgcggtcgc cgcctgaacc
    26281 ggagctaatg ggccggcacg cataccacgg cgaccacccg ctggcgattg ctgcgcttgt
    26341 ttcgtcgccc ctggggttac actctgggag tcattgcggc cttgccagaa ccagaatgcg
    26401 gcgatggcgg cgataaccac cacgattacg attacccaac gggatttata actgcctttc
    26461 atcgttaaga gtttctcttc ctgaaacgtt tcgcggaatg atactagtgt agccagccaa
    26521 taagggagaa aaatggagga attatgaaac actgtcggga atcgtctgaa tgacgggcac
    26581 atttgcgagc acgcatccag taataacaca tgaaactatt ttatctacgc gttagcgata
    26641 gactgcttgc atggcgaaag gagataagcc gacgatttca gcgggacgct gaaacggaaa
    26701 agcccctccc gaggaagggg ccttaaataa ggaaagggtc atgatgaaga tactcatcat
    26761 cgtggtgctc ttagtgatta gctacccagc atactaagac taccagggcg ggggaaaccc
    26821 cgctctaccc tcactcctga aagtatgcct tcacgataag attgtcaatc cgcaggcttt
    26881 gtagtctgcg atcctgccag caaatattct ttgcgagtcg ttacgcaata atcacacttg
    26941 aaactatttt atccgcaagt cagcgataga ctgcatacag ggcgaaagga ggtaagccga
    27001 agatttcagc gggacgctga aacgggaaag cccctcccga ggaaggggcc ttaaataagg
    27061 aaagggtcat gatgaagcta ctcatcatcg tggtgctctt agtcattagc tggccagctt
    27121 actaagacta ccagggcggg ggaaaccccg ctctaccctc actcctgaaa ttatgtcact
    27181 ccgcccttag tgtcaatcga cggctctccg ttcttgcgag gcgaatagca aaattttctg
    27241 cgccgccggg cctaattctg aaagtttctt cgtttgctct tatttcatta ttgcaatcgg
    27301 tatgataccg gacctcaata attctctaag aaaggatttc ttatgagcga acaaatcacg
    27361 tttgccacca gcgattttgc cagtaaccca gaaccacgtt gcccctgcat tttgctgctg
    27421 gatgtttctg gttctatgaa cggtagaccc attaatgaac ttaacgccgg attggttacc
    27481 tttcgcgatg agctgcttgc cgattcgctg gccttaaaaa gagtggaact cggcattgtg
    27541 acgttcggcc cggtgcatgt ggaacaaccc tttaccagcg ccgctaattt tttcccgccc
    27601 atcctgtttg cccagggcga tacaccaatg ggcgctgcca ttaccaaagc cctggatatg
    27661 gttgaggagc ggaaacgtga atatcgtgcc aatggtattt cctattatcg tccgtggatt
    27721 ttcctgatca ccgatggcgc accaaccgat gagtggcagg ctgccgccaa caaagtgttt
    27781 cagggggaag aagataagaa gtttgccttc tttaccatcg gtgttcaggg ggcggatatg
    27841 aagacgctgg cccaaatcag cgtccgtcag cctttgtctc tgcagggatt acaattccgt
    27901 gaactgttta gctggttatc cagttcgctg cgttcggttt cccgctccac gccgggaacg
    27961 gaagttgtgc tggaagcgcc aaaaggctgg acgtcagtgt gagctggcga ctggtctatg
    28021 catcagccgt tggaacgtcg catattagcg ctgatttgcc ttgccaggat gcctgccaga
    28081 tgcaggttgc ctggctaaat gaccagcagc cattattgtc ggtattcgtc gctgatggcg
    28141 caggcagcgt ctcgcagggg ggcgaaggtg cgatgctcgc cgtcaatgaa gcgatggctt
    28201 atatgtcgca aaaagtgcag ggcggggaat tggggcttaa tgatgtcctc gcaacggaca
    28261 ttgtgctgac cattcgtcag cgactttttg ccgaggcgga agccaaagaa ttagcggttc
    28321 gcgattttgc ctgcacgttt ttgggattga tttcatcagc taatggcacc ttgattatgc
    28381 agattggcga tggcggcgtg gtggtagatt tggggcacgg tttgcaactg ccgctcacgc
    28441 cgatggtggg tgaatatgcc aatatgacgc acttcattac cgatgaagat gccgtttcca
    28501 gactggaaac tttcaccagc actgagcgtg cgcataaagt tgcagcattt acggatggta
    28561 ttcagcgact ggcgttaaat atgctggata attctccgca tgtgcctttc tttaccccgt
    28621 ttttcaatgg actggcgtca gcaacgcagg aacaactcga tttattacct gaattgttaa
    28681 agcagttttt gtccagcccg gcggtaaacg agcgtacgga tgatgataaa acgttggcac
    28741 tggctatgtg gttaccatga aacccacttt atatactgct actggtgagt gcgttacgcc
    28801 aggccgtgaa ctgggcaaag gcggcgaagg tgcggtttat gatatcgagg agtttgtcga
    28861 tagcgtcgcc aagatttatc acacgccgcc acccgcctta aaacaggaca aacttgcctt
    28921 tatggctgcg acagctgacg cgcagttgtt gaattatgtc gcctggccgc aggcaacgct
    28981 tcacggtgga cgaggcggaa aagttatcgg ttttatgatg ccaaaagttt ctggtaaaga
    29041 accgattcat atgatctata gcccggcaca tcgtcgccag agttaccctc attgtgcgtg
    29101 ggattttcta ctctatgttg cgcgcaatat tgcttcatct tttgctactg ttcatgagca
    29161 cgggcacgtc gtgggtgacg taaaccagaa cagctttatg gtaggtcgcg acagcaaagt
    29221 ggtgttgatt gatagcgact cctttcagat taatgccaat ggtacactgc atttatgcga
    29281 agtcggcgtg tcgcatttta cgccgccaga gctgcaaacc atgccatcat ttgtcggctt
    29341 tgaacgcaca gaaaatcacg ataattttgg ccttgcgttg ctgatatttc acgtcttgtt
    29401 tggtgggcgg catccttatt ccggtgtacc gcttatctct gatgcgggta atgcgctgga
    29461 gatggatatt gcccatttcc gttatgccta cgcgtcagat aatcagcgac gtggtttaaa
    29521 accgccgcca cgatcgattc cgctgtcgat gttaccgggc gatgttgaag ccatgtttca
    29581 gcaggcattc acggaaagtg gcgtggcaac cgggcgtccg acggcaaaag cgtgggtagc
    29641 ggcacttgat ttactacgcc aacagttaaa gaaatgtacc gtttcggcaa tgcatgttta
    29701 ccccgctcat ttgactgact gcccgtggtg tgcgctggat aatcaaggcg ttatctattt
    29761 tattgatctc ggcgaagagg tcattaccac cggcggtgat tttgtgctgg cgagagtctg
    29821 ggcgatggtg atggcatcag tagcaccgcc agcattgcaa ctgccattac ccgatcattt
    29881 ccaaccgact ggcaggccgc ttccttcagg cctgttacgg cgcgaataca tcattctgat
    29941 tgagatcgca ctgtcagcgt tatcgctgtt gctttgcggc cttcaggcag aaccgcgtta
    30001 tattattttg attcctgtgc tggcggctat ctggattatt ggcagcctga caagcaaagc
    30061 gtacaaagca gaaatccagc aacgccgtga ggcgtttaat cgcgcgaaaa tggactatga
    30121 ccatttagtc agccagatcc agcagttggg cgggctggaa ggttttatcg ccaaacgggc
    30181 gatgctcgaa aaaatgaagg acgaaattct cgggttaccg gaagaagaga aacgcgctct
    30241 ggtagcactt cacgacaccg caagggaacg gcagaagcag aagtttctgg agggattttt
    30301 tattgatgtt gcctctattc ccggcgttgg ccctgcgcgt aaagcggcgt tacggtcctt
    30361 tggtattgaa acagcagcgg atgttacccg acggagcgtt aagcaagtga aaggttttgg
    30421 tgatcatctg acccaggcgg ttatcgactg gaaagcgagc tgtgaacgcc gttttttgtt
    30481 caggccgaac gaagcggtaa cgcctgcaga cagacaagcg gtaatggcaa aaatggccgc
    30541 caaacgacat cggctggaat cggcgctgac tgtcggtgcg acagagttgc agcgattccg
    30601 ccttcatgcg ccagcacgga ccatgccgtt gatggaaccg ctacgtcagg cggcagaaaa
    30661 actggctcag gcgcaggcag atttaagccg ttgctgagcc atttttcaat taacgaaaca
    30721 ccacttccgc ccagcgtgcc agcccggcgg tgacggagcc aaagtcatcg ccgcctgcaa
    30781 ccggaatgcc cggcaactgt tctgccagcg cttttttaat cagcggcgaa cgggcgctac
    30841 cgccggtcag gtagataacg tccggttttt cccgggcgtt atccagcgcc agttgcacct
    30901 gttccagaat ccgcgccagt ggctggctga gggcgctttc cagcccttgc tggctaatca
    30961 gcgtagccag ttcatcgctg ataaacggca gtgaggcgcg ggtttccgct acacttgaaa
    31021 gggcgatttt gctctcttct gcgctacgca ccaggcgata gcttaaacgc tgacgccaga
    31081 ctttctgtaa cagggccact ttttccggtt cacgggcatc gcgtaccaga tcgttaagca
    31141 gacgaccgtt agcactactg tagaaatcac tctgcgcagg aacgtcgttg atcgcaaccg
    31201 cattccacca cggcagaatc ggcagggcga tgcctttttc ggtttcgcca cccatgccca
    31261 gtaacggcat caggttctta aacgccagtg cgatatccag atcgttacca ccaatacggc
    31321 aaccactatg acccagcagg ctggcttcac gatcgagacg cgaacgccat tgcggcccca
    31381 tcagcagcaa tgaacagtca gtcgtaccac cgccgatatc cactaccagc actcgttttt
    31441 cttcctgcaa ggtggcttcg taatccagcc cagccgcgac cggctcgtac tggaatacca
    31501 catccttaaa cccggcacgc ttcgccgcgc gttccagaat cccttgcgcc tgggtgtttg
    31561 cttcatcacc gcccagcccc tggaagttga tcgggcgacc aatcaccgcc tgagtaattg
    31621 cctccggcag ctgcgcctgc gcctgttggc gaatgtgcaa catcattgcg cagaccagat
    31681 cctcaaacag cgctacctgc tgcggtttta agccgctggc accgaggaac gattttggtg
    31741 atttcacaaa ccacacttct tctggatcat caatgtactg tgccagtgag gaaagaccga
    31801 actgcacgct tttcgccgta acatcgatat cttcttcgcg gttataacga atcgcccgac
    31861 gcaacagcgc ctgcgtttca tcgtcgtctg ccggaacatc atgatggcgg tacagccatt
    31921 cgcttaccgc ttcacgcgtt ggcgcgcaaa gcattgaagg cagcagcgtg ctgtcgtttt
    31981 ccatttttag caaatgcggt ttaccgtcgc gcatgaccgc cactgaacag tttgctgtac
    32041 cgtaatcaaa accaataaac acgaaataat ccccatgccg gtgaagaagg ggcgtgactt
    32101 tagcgaaatg ttgccgtcgc gacaaccgga atatgaaagc aaagcgtaac gtctgaataa
    32161 cgtttatgct gaaagcggat taacaaggag atgcgatgta taccctgaac tggcagccgc
    32221 cgtatgactg gtcgtggatg ttgggatttc tcgccgcccg tgcggtgagc ggcgtggaaa
    32281 cggtcgcgga cagttattat gcccgtagtc tggcggtagg cgaatatcgc ggcgtggtga
    32341 ctgctattcc ggatatagcc cgccatactc tgcacataaa tttaagtgca ggtttagaac
    32401 ctgttgccgc tgagtgtctg gcgaaaatga gccgcctgtt tgatctgcaa tgtaacccgc
    32461 agattgttaa cggtgcgttg ggcaagttag gcgcggcgcg gccaggattg cgtttacctg
    32521 gttgtgttga cgcttttgag cagggcgtgc gggcgatttt aggccaactg gtgagcgtgg
    32581 cgatggcggc aaaattgacc tccagagtgg cacagcttta tggcgaacgg ctggatgatt
    32641 ttccggatta tgtctgcttc cccacgcctc agcggctggt ggtagccgac ccgcaggcat
    32701 taaaagcgtt aggtatgccg ttgaaacggg cagaggcgct gattcatctg gcaaatgcgg
    32761 cgctggaggg caccttacca atgacaatac cgggcgacgt agaacaagcg atgaaaacgc
    32821 tgcaaacctt tccgggtatc gggcgctgga cggcaaatta ttttgctttg cgtggctggc
    32881 aggcgaaaga tgtttttctg ccggatgatt atctgattaa acagcgattt ccgggaatga
    32941 cgccggcaca aatccgccgt tatgccgaac gctggaagcc ctggcgttct tatgcgctgt
    33001 tgcatatctg gtatacggaa ggctggcaac cagacgaagc atgacgttgg tcgccggatg
    33061 cggtgcgagc gccttatccg gcctggggaa ttagcgattt gtaggcctga taagacgcgt
//
LOCUS       DAZKHK010000047        33007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKHK010000047 DAZKHK010000000
VERSION     DAZKHK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.047"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..830
                     /gene="yfeN"
                     /locus_tag="ACHOS5_002210"
     CDS             66..830
                     /gene="yfeN"
                     /locus_tag="ACHOS5_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP9777025.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(869..1795)
                     /gene="yfeR"
                     /locus_tag="ACHOS5_002211"
     CDS             complement(869..1795)
                     /gene="yfeR"
                     /locus_tag="ACHOS5_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9777026.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRDQGNALMAGREGDPLYQI"
     gene            1885..2883
                     /gene="yfeH"
                     /locus_tag="ACHOS5_002212"
     CDS             1885..2883
                     /gene="yfeH"
                     /locus_tag="ACHOS5_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP9777027.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2880..3098)
                     /gene="ypeB"
                     /locus_tag="ACHOS5_002213"
     CDS             complement(2880..3098)
                     /gene="ypeB"
                     /locus_tag="ACHOS5_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP9777028.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGHRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKNEGLTQLVQPLKRPL"
     gene            complement(3100..5115)
                     /gene="ligA"
                     /locus_tag="ACHOS5_002214"
     CDS             complement(3100..5115)
                     /gene="ligA"
                     /locus_tag="ACHOS5_002214"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9777029.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(5186..6184)
                     /gene="zipA"
                     /locus_tag="ACHOS5_002215"
     CDS             complement(5186..6184)
                     /gene="zipA"
                     /locus_tag="ACHOS5_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP9777030.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6414..7175
                     /gene="cysZ"
                     /locus_tag="ACHOS5_002216"
     CDS             6414..7175
                     /gene="cysZ"
                     /locus_tag="ACHOS5_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP9777031.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7360..8331
                     /gene="cysK"
                     /locus_tag="ACHOS5_002217"
     CDS             7360..8331
                     /gene="cysK"
                     /locus_tag="ACHOS5_002217"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9777032.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8715..8972
                     /gene="ptsH"
                     /locus_tag="ACHOS5_002218"
     CDS             8715..8972
                     /gene="ptsH"
                     /locus_tag="ACHOS5_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP9777033.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            9017..10744
                     /gene="ptsI"
                     /locus_tag="ACHOS5_002219"
     CDS             9017..10744
                     /gene="ptsI"
                     /locus_tag="ACHOS5_002219"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP9777034.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10785..11294
                     /gene="crr"
                     /locus_tag="ACHOS5_002220"
     CDS             10785..11294
                     /gene="crr"
                     /locus_tag="ACHOS5_002220"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9777035.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11337..12188)
                     /gene="pdxK"
                     /locus_tag="ACHOS5_002221"
                     /gene_synonym="thiJ"
     CDS             complement(11337..12188)
                     /gene="pdxK"
                     /locus_tag="ACHOS5_002221"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP9777036.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12293..12661
                     /gene="yfeK"
                     /locus_tag="ACHOS5_002222"
     CDS             12293..12661
                     /gene="yfeK"
                     /locus_tag="ACHOS5_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP9777037.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12664..13575)
                     /gene="cysM"
                     /locus_tag="ACHOS5_002223"
     CDS             complement(12664..13575)
                     /gene="cysM"
                     /locus_tag="ACHOS5_002223"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP9777038.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13710..14807)
                     /gene="cysA"
                     /locus_tag="ACHOS5_002224"
     CDS             complement(13710..14807)
                     /gene="cysA"
                     /locus_tag="ACHOS5_002224"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP9777039.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14797..15672)
                     /gene="cysW"
                     /locus_tag="ACHOS5_002225"
     CDS             complement(14797..15672)
                     /gene="cysW"
                     /locus_tag="ACHOS5_002225"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP9777040.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWCL
                     ENQEKRAQQEEHHEH"
     gene            complement(15672..16505)
                     /gene="cysT"
                     /locus_tag="ACHOS5_002226"
     CDS             complement(15672..16505)
                     /gene="cysT"
                     /locus_tag="ACHOS5_002226"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP9777041.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16505..17521)
                     /gene="cysP"
                     /locus_tag="ACHOS5_002227"
     CDS             complement(16505..17521)
                     /gene="cysP"
                     /locus_tag="ACHOS5_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP9777042.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(17679..18470)
                     /gene="ucpA"
                     /locus_tag="ACHOS5_002228"
     CDS             complement(17679..18470)
                     /gene="ucpA"
                     /locus_tag="ACHOS5_002228"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP9777043.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            18750..19646
                     /gene="murQ"
                     /locus_tag="ACHOS5_002229"
     CDS             18750..19646
                     /gene="murQ"
                     /locus_tag="ACHOS5_002229"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9777044.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            19650..21074
                     /gene="murP"
                     /locus_tag="ACHOS5_002230"
     CDS             19650..21074
                     /gene="murP"
                     /locus_tag="ACHOS5_002230"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP9777045.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            21077..21163
                     /locus_tag="ACHOS5_002231"
                     /pseudo
     CDS             21077..21163
                     /locus_tag="ACHOS5_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21252..22151)
                     /gene="yfeX"
                     /locus_tag="ACHOS5_002232"
     CDS             complement(21252..22151)
                     /gene="yfeX"
                     /locus_tag="ACHOS5_002232"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP9777046.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22247..22822)
                     /gene="yfeY"
                     /locus_tag="ACHOS5_002233"
     CDS             complement(22247..22822)
                     /gene="yfeY"
                     /locus_tag="ACHOS5_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP9777047.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(22883..23332)
                     /gene="yfeZ"
                     /locus_tag="ACHOS5_002234"
     CDS             complement(22883..23332)
                     /gene="yfeZ"
                     /locus_tag="ACHOS5_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP9777048.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23319..23744)
                     /gene="ypeA"
                     /locus_tag="ACHOS5_002235"
     CDS             complement(23319..23744)
                     /gene="ypeA"
                     /locus_tag="ACHOS5_002235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP9777049.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDISL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            23958..24827
                     /gene="amiA"
                     /locus_tag="ACHOS5_002236"
     CDS             23958..24827
                     /gene="amiA"
                     /locus_tag="ACHOS5_002236"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP9777050.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            24831..25730
                     /gene="hemF"
                     /locus_tag="ACHOS5_002237"
     CDS             24831..25730
                     /gene="hemF"
                     /locus_tag="ACHOS5_002237"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP9777051.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(25736..26788)
                     /gene="eutR"
                     /locus_tag="ACHOS5_002238"
     CDS             complement(25736..26788)
                     /gene="eutR"
                     /locus_tag="ACHOS5_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP9777052.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(26834..27334)
                     /gene="eutK"
                     /locus_tag="ACHOS5_002239"
     CDS             complement(26834..27334)
                     /gene="eutK"
                     /locus_tag="ACHOS5_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP9777053.1"
                     /translation="MINALGLLEVDGMVAAIDATDAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27347..28006)
                     /gene="eutL"
                     /locus_tag="ACHOS5_002240"
     CDS             complement(27347..28006)
                     /gene="eutL"
                     /locus_tag="ACHOS5_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP9777054.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVANIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28016..28903)
                     /gene="eutC"
                     /locus_tag="ACHOS5_002241"
     CDS             complement(28016..28903)
                     /gene="eutC"
                     /locus_tag="ACHOS5_002241"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP9777055.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(28924..30285)
                     /gene="eutB"
                     /locus_tag="ACHOS5_002242"
     CDS             complement(28924..30285)
                     /gene="eutB"
                     /locus_tag="ACHOS5_002242"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP9777056.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30297..31700)
                     /gene="eutA"
                     /locus_tag="ACHOS5_002243"
     CDS             complement(30297..31700)
                     /gene="eutA"
                     /locus_tag="ACHOS5_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP9777057.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31697..32923)
                     /gene="eutH"
                     /locus_tag="ACHOS5_002244"
     CDS             complement(31697..32923)
                     /gene="eutH"
                     /locus_tag="ACHOS5_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP9777058.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGNVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEAEAQS"
BASE COUNT         7745 a         9081 c         8411 g         7770 t
ORIGIN      
        1 gccgctttta atcaaccttt ttattaagca atcaactaat aacgatcggc atagggaatt
       61 atgctatgaa aaaacatctt tttactctga cactctcttc tgtattggcg ataccagcag
      121 tatcccatgc agaatttaaa ggcggttttg ctgatatagg tattcattat cttgactgga
      181 ctagcagaac cactgagaaa tcgtcgacca aatcacacaa agatgatttt ggatatctgg
      241 aactggaagg cggcgctaac tttagttggg gagaaatgta cgggttcttc gactgggaaa
      301 acttttataa tgatcgtcat gataaaccgg gtagcgagca gcgttataca tttaaaaata
      361 ccaaccgtat ttacctgggt gacaccggat ttaatctcta cttgcatgcg tatggcacct
      421 acggttctgc gaatcgtgtg aatttccatg acgatatgtt cctttacggg atcggctaca
      481 atttcaccgg cagcggttgg tggttcaaac cgttctttgc aaaacgctat acagaccaaa
      541 cctattatac cggcgataac ggttatgtcg ccggttgggt tgcaggttac agctttatgc
      601 tgggcagtga gaaattcacc ctcaccaact ggaacgagta cgagtttgat cgtgatgcca
      661 cctacgcggc aggtaatggc ggcaaagaag gtctgaacgg tgccgtcgca ctttggtgga
      721 atgcaacttc acacatcacc acagggattc agtatcgtta tgcggatgac aaactgggcg
      781 aagattttta ccaggatgcg ataatctact ccatcaaatt taatttctga ttgattactg
      841 ccggagctcc cggcagtatg aattcactct atatctgata caacggatcc ccttcccgcc
      901 cagccattag cgcattgccc tggtctcgta ctacgtccca taacgcttgt gcggcggtgg
      961 aaagtgaacg gttcttcctg cgcaccagca tcaactggcg ttcaaccact ggcgtaatgc
     1021 gctttaccac cagtgggcta ccttcaggca atggtagcgc cagtgcggga agaatactga
     1081 tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg atgaccaatc tcctgcacaa
     1141 tattcgcctg aataccgttg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg
     1201 cgtaatcctg caacaccaat ttcgctccct gtaatgcctg ccagggtaca taatcttcca
     1261 cggccaaagc gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac
     1321 attgcagatc gcccactggg cctggatcga tgacgatgcc aaaatcgaca tctccctggc
     1381 gaatactttc catcacccac tgctgcggac gatcgtgcag gacaaactga atatctggat
     1441 agcggcgatg gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg
     1501 cagcgacccg aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc
     1561 tgttaagttc atccagcagt cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa
     1621 gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagctcct
     1681 tcacactgtg actcactgcc gactggctca ggccgatacg ctctcctgca cgactaaaac
     1741 ttttctcctg cgctactgtg acgaaaacct ttaattgctt taaagaataa ttcatctgtt
     1801 tttttcatga atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag
     1861 aaccatcgat catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta
     1921 accctgatca cagtggtgtt gctggcctct ttctttccgg ccagaggcga tttcgtcccc
     1981 ttctttgaaa atctgaccac cgcagcgata gccctgctgt tttttatgca cggcgcgaag
     2041 ttgtcgcgtg aggcgattat tgctggcggt ggccactggc gactgcattt gtgggtaatg
     2101 tgcagcacct tcgtgctgtt tccgattctg ggcgtgctgt ttgcctggtg gaaaccggta
     2161 aatgtcgacc cgatgctcta ctccggtttt ctctacttgt gcattctccc cgcgaccgtg
     2221 cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg ttgcggcggc ggtttgttct
     2281 gcgtcagcat ccagcctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg
     2341 aatgttcacg gtgcaggggg cagccttgag caggtcggta aaattatgct gcaactgttg
     2401 ctgccatttg tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgtaat
     2461 aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacggcattc
     2521 agcgaagccg tcgttaacgg tatctggcac aaagttggct ggggatcatt gctgtttatc
     2581 gtggtggtca gttgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc
     2641 cgactgggct tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt
     2701 ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg
     2761 gtgctgcccc tgatgatttt ccatcagatc caattgatgg tctgtgcggt gctggcgcgt
     2821 cgatacaaac gccagaccga acagttacag gcgcagcagg aaagcagcgc cgataaagct
     2881 taaagcggac gcttcagggg ctggaccagt tgcgtcagcc cctcgttttt aatcagcagc
     2941 gtgatttgca ttagctcacc gagcttccct gcagggaact catctttgcg ggcaaaccac
     3001 agcagatact cctccggcag gtcgattaag cgatgccctt tgtatttgcc aaacggcatt
     3061 atcgtattgg ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag
     3121 catttccgtt tcgtcgatga cttcaatgcc cagttcctgc gccttcgcca gtttagatcc
     3181 tgcagcttca cccgctatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc
     3241 ccccagttcg accagtcgag ctttagcgtc atcacgcgac atctggctta agctgcccgt
     3301 aagcaccacg gttttaccag caaacggact gtcaatctct tccgcattga taacgatcgg
     3361 ctcaggccag tgaacacctt ccgccaacag ctcgctgatg acattgcggt tgctttcttc
     3421 ggcaaagaag ttgtgaacgt gggaggcaac gacaattcca acatcaggca ccttttgcag
     3481 ctcttcaatc gacgcggctt ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc
     3541 tgcggtagcc tcgccgactt cacggatgcc aagtgcatag aggaagcggg caaaggtggt
     3601 ttctttcgct ttttccagcg cgttaaccac gttttgtgcc gatttcggcc ccatacgctc
     3661 cagtccggtc agtttgcctg cggtgagttt gaacagatcc gccggagtgt ggacatattc
     3721 tttttcaacc agttgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg
     3781 ggaaacaaag tgtttcagag actctttacg ctgcgcaccg caaatcaggc caccggtaca
     3841 gcgggcaacc gcttcacctt ccacacgctc gacgtcagaa ccacataccg gacaatgcgt
     3901 cgggaataca acctcacggg tatcttccgg gcgttcagaa agcacgacgt taaccacctg
     3961 cgggatcaca tcgcctgcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg
     4021 ttcgatttcg tcagcattat gcagtgtagc gttactcacc aacacgccag caacatggac
     4081 aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc
     4141 acgcacaaag gtcatctgtt cctgcgccgg gaatttaaac gctaccgccc agcgcggggc
     4201 acgcgcgaca aagccaagct gctcctgctg tgccagtgag ttgaccttaa tcaccacgcc
     4261 gtcgatatca aagcccagcg tcggacggtc ttcttctact ttgtggtaga acgccagcac
     4321 ttcttccgcc gattcacaaa gcgttacccg atcgctgacc ggcaaacccc actttttaaa
     4381 ttgcagtaaa cggccaagat gagtatccgg cagctcgcca ccttccagaa caccgacgcc
     4441 gtagcagaag aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga
     4501 accagctgcc gcattacgtg ggttagcaaa cactttcccg cccgtgcgtc gcgcatcttc
     4561 gttaatcttt tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc
     4621 cgggatattc tcaccgtgta gcttcagcgg aatggcgcga atagtacgca cattagacgt
     4681 gatatcttcc ccggtggtgc catcgccacg cgtcgcggca ctgactaaaa cgccattttc
     4741 atacagaata ctgacggcaa gaccatccag cttcagctca cagcaccagg tgactttctc
     4801 gttgcttttc agacggtcct gcactcgttt gttgaaggca agaaagcttt cttcatcaaa
     4861 aacgttatcc agcgacagca ttggcacttc atggcggatc tggctgaaag ccgccagcgg
     4921 cgcagcacct acacgttggg taggcgaatc aggcgtaatc agttctggat gtttggtttc
     4981 cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc
     5041 catcacatga taaagatatt catgatggcg aagcgtcgtt cgcagttctg tcagttgttg
     5101 ttcgattgat tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa
     5161 aaggagttaa tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt
     5221 cctggtactc gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcactacac
     5281 cgcccacttc atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct
     5341 cgtcaccgta agacggtacc tgcataaaga tagtgacacc cggagtagtg aaatccttca
     5401 tttcaggatc aaaggttccc ggtttcacca tattcgccag gctgaataac gccgggccgc
     5461 tgccatccgg gctaagatga cgatggtaaa tgttcatatc gccaaaaatg aagcccgctt
     5521 gttgaatgct gttaagaagc agttcaccgt ttaactcgct accgtgatgc gccgcgacgt
     5581 tcatgataat caccgcttct ttgcgcttcg gtttatccgt aactggagcc ggttccgcta
     5641 caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg aaaagcctgt tgtgccggtt
     5701 gcggtgctga atgcactggc tgcggcgctg acgcgacctg tggcgaaact ggctgctgca
     5761 acggctgttc aggctgcggc tgataggcag gctgctgcac gggttgctgc accggctgcg
     5821 ttggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg accggttgac
     5881 gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga cgagcagcct
     5941 catgctcctg ggcgttagcc ggggcatgat tcacgcggtg aacacgaacc tcaccaacgc
     6001 cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac ttcattcgtt
     6061 ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag aaaccatgta
     6121 ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc aaatcctgca
     6181 tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta ctcactaaga
     6241 gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac aaagatagat
     6301 gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc gcaattaccc
     6361 ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg attatggttt
     6421 catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc tggaagctcg
     6481 tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat attttgttga
     6541 tggggggcgc attctggtgg ctctttacgc aactcgatgt ctggatcccg actctcatga
     6601 gttatgttcc ggactggtta caatggctga gctatctgtt gtggcccctg gctgtcatct
     6661 ctgtgctgtt agtatttggt tatttctttt caacgattgc taactggatt gccgctccgt
     6721 tcaacggttt attggctgaa caactggaag caagactgac tggcgctaca ccgccagata
     6781 ccggaatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa tggcaaaaat
     6841 ttgcctggta tctgccgcgc gcaattgtat tgctaattct ttacttcatc cctggcattg
     6901 ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta gccatccagt
     6961 attgtgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc accgccctgc
     7021 gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt acgatgatcc
     7081 cgctgcttaa tctgttcatt atgcccgttg ccgtttgtgg cgcgacggcg atgtgggtcg
     7141 attgctatcg cgataaacac gccatgtggc ggtaacaatc taccggttat tttgtaaacc
     7201 gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat gtcattattt
     7261 cccttctgca tatagatatg ctaaatcctt acttccgcat attctctgag cgggtatgct
     7321 acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat ttttgaagat
     7381 aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg taacggacgc
     7441 attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg tatcggtgct
     7501 aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga actggttgaa
     7561 ccaaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg cggttacaaa
     7621 cttaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct gaaagcgtta
     7681 ggtgcaaacc tggtgctgac cgaaggtgct aaaggcatga aaggcgcaat ccaaaaagca
     7741 gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt cagcaatccg
     7801 gcaaaccctg aaattcatga aaaaaccacc ggtccggaga tctgggaaga taccgacggt
     7861 caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg cgtcagccgc
     7921 tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga gccaaccgat
     7981 tctccagtta tcgcccaggc gctggcaggt gaagagatta aacctggtcc gcataaaatt
     8041 cagggtattg gcgctggttt tatcccggct aaccttgatc tcaagctggt cgacaaagtc
     8101 attggcatca ccaatgaaga agccatttct accgcacgtc gtctgatgga agaagaaggc
     8161 attcttgctg gtatctcttc tggagcagct gttgctgcgg cgttgaaact acaagaagat
     8221 gaaagcttta ccaataagaa tattgtggtt attctgccgt catcgggtga gcgttattta
     8281 agcactgcat tgtttgccga tcttttcact gagaaagaac tgcaacagta atgccagctt
     8341 gtaaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc ttcaaacttt
     8401 cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac taattatttt
     8461 gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta tgatttggtt
     8521 caattcttcc tttagcggca taatgtttaa tgacgtacga aacgtcagcg gtcaacaccc
     8581 gccagcaatg gactgtatta cgctcttcgt gcgtcgcgtc tgttaaaaac tggcgctaac
     8641 aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact aaacctataa
     8701 gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac ggtctgcaca
     8761 cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa attactgtga
     8821 cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact ctgggcctga
     8881 ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa gcggttgaac
     8941 atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa aaatcagtca
     9001 caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg ctttcggtaa
     9061 agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt ctgccgacca
     9121 ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag cccagttgga
     9181 aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca tctttgaagg
     9241 gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc tgattaaaga
     9301 taagcacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg cttctgccct
     9361 ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg atatcggtaa
     9421 gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca ttcaggatga
     9481 agtcattctg gttgccgctg acctgacgcc gtccgaaaca gcacagctga acctgaagaa
     9541 ggtgctgggt ttcatcaccg acgcgggtgg tcgtacttcc cacacctcta tcatggcgcg
     9601 ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg tgaaaaatga
     9661 cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa ccaacgaagt
     9721 tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag agcttgctaa
     9781 actgaaggat ctaccggcta ttacgctgga cggtcaccag gtagaagtgt gcgctaacat
     9841 tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg ttggtctgta
     9901 tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag aacagtttgc
     9961 tgcttacaaa gcagtggctg aagcgtgtgg ctctcaggcg gttatcgttc gtaccatgga
    10021 catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga acccgttcct
    10081 cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg atcagctccg
    10141 cgctatcctg cgtgcctctg ctttcggtaa attgcgcatc atgttcccga tgatcatctc
    10201 tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg aactgcgcga
    10261 cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa caccggctgc
    10321 cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg gcaccaatga
    10381 tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac acctttacca
    10441 gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt ctcatgctga
    10501 aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta cacttctgtt
    10561 gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc gcattaagaa
    10621 gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg ctcttgctca
    10681 accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa aaacaatctg
    10741 ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt ttgttcgata
    10801 aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag atcattgctc
    10861 cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt gcggaaaaaa
    10921 tcgttggtga tggcattgct atcaaaccaa cgggtaacaa aatggtcgcg cctgtagacg
    10981 gcaccattgg taaaatcttt gaaaccaacc acgcgttctc tatcgaatct gatagcggcg
    11041 ttgagctgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa ggcttcaagc
    11101 gtattgctga agaaggtcag cgcgtgaaag ttggtgatac tgtcattgaa tttgatctgc
    11161 cgctgctgga agagaaagcc aagtcaaccc tgactccggt tgttatctcc aacatggacg
    11221 aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc ccggttatcc
    11281 gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc atttttttat
    11341 gcttcagcca gaggcggcaa aatcaattca tcgctctcat gctgctgggt atagcgcatc
    11401 acttccagta cgcgcaaccc cgcgcggtgt accgcatcgg ttaacgcctt ccctttcagc
    11461 aaaccactga taagctgagc acaaaacagg tcgccagtcc ctttcaggtc agttttaacc
    11521 cgggaatggg aaatgacatt cacgctgtcg gcactgacca ccaccacctg catctcctga
    11581 ttttcttcat tgccggaggc gctggtaatc accacccact ttaatgtgtc tgaaagcaga
    11641 ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat ctttaccggt caggatttcc
    11701 agctcaaaga tattgggggt aattccctgt gccagcggca gtaaatattg tcgatacgcc
    11761 tcgggaaggt caggtttgac ataaattccg ctatcaatat cgccaatcac cggatcgacc
    11821 atgatcaata ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg
    11881 atttgcgatg ccgttcccat atagcccgtg gttacagcac gaagttggcg cagcgcatca
    11941 cgctcctgaa gcgcacgcaa ataaccgcta aaccattcat ccggaatcgc accaccgtag
    12001 aaagtgtcat aatgcggcgt attgctcagc aataccgtcg gcacggcaaa gacattcagg
    12061 ccgttctgtt tgatagcagg caccgcaatg ctgttgccta cgctgccgta cactacctgc
    12121 gactgcacgg caacgatatc cgcctgcagt gccctgctct tatcgttaaa caacaacaaa
    12181 ctactcattt aattttttct ccttgccgat gatcctcatc gtattccagc cgaaacttta
    12241 cctgattctg gcagtcaaat cggctattac aaaacaagga taaggtaatt cagtgaagaa
    12301 aatcatttgt ctggtcgtta cgctgctaat gacactcccc gcttatgcga agttaacggc
    12361 ccatgaagaa gcccgtatca acgccatgct ggagggatta gcacagaaaa aggatttgat
    12421 atttgtgcgc aacggtgatg aacatacctg cgatgaagcg gtttctcatc tgcgcctgaa
    12481 gctgggcaat acccgtaatc gcattgatac agccgagcag tttattgaca aggtggcctc
    12541 gtcgtcatcg attaccggga agccgtatat cgtgaagatg cccggtaaga gcgatgagaa
    12601 cgcacagcct tttttacatg cgttaattgc gcagacggat aaaacggtgc ctgcgcaata
    12661 atcttaaatc cccgccccct ggctaaaatg ctcttcccca aacaccccgg tagaaaggta
    12721 gcgatcgcca cgatcgcaga tgatcgccac caccaccgcg ccagggttag cttttgccac
    12781 ccgcagtgct ccggcaaccg cgccgccgga gcttacgccg cagaaaatcc cttcccgcac
    12841 tgccagttcg cgcatggtgt tttccgcatc gcgctgatga atatccagca cctcatccac
    12901 cagagaagca ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa tgccgggaat
    12961 actgctgcct tcttccggct gcaggccaac aatggtcacc ggtttggatt gttcgcgcat
    13021 aaagcgtgac acgccggtga tagtgccggt tgttcccatg ctggagacaa aatgggtaat
    13081 acgcccgcca gtttgctgcc agatttccgg cccggtagtg gtgtaatggg cgtaagggtt
    13141 atcgggatta ttgaactgat cgagcaactt tccttcgccg cgattcgcca tttcaagcgc
    13201 cagatcgcgt gcgccttcca tgccctgttc tttggtgacc agaatcagtt ctgcaccgta
    13261 ggcacgcatc gccgcacggc gttcctggct catgttgtcg ggcatcagca atttcatgcg
    13321 atagccttta agcgccgcaa tcattgccag cgcaatgccg gtgttaccac tggtggcttc
    13381 gatcagcaca tcacccggtt taatctcgcc gcgcttttcc gcctcgacaa tcatcgaaag
    13441 cgccgcacgg tcttttaccg atcccgccgg gttattgcct tccagtttta accacacttc
    13501 actgccgtta tccggcgcca ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg
    13561 ttctaatgta ctcacgatct ctgtccatac gtggtgttgc ctgatgcgac gcttacgcgt
    13621 cttatcagac ctacaggtta caaaaccttg ccattaaaaa gcccggatcg cgggaagcgc
    13681 ctccgggcgt ttaacattca ctcaacctat caggcgcttt gtgcgagagc aagttcctca
    13741 tcgcgggttt caatacgctc atcgccgtta tacagccgcg catgttgcag accaacgtat
    13801 aaacgatcgc cacgctgcgg ggcatcgtcg ccatgcatca cgaccgtcag cggttcgttg
    13861 taccacccca gcggctgcac cactaattgg gtgtagtgac ctttcgggct ggcttccagt
    13921 acctgtacgg gtagcggcga atcgaggctg gtacggcggc tgatatccac ttcccacggg
    13981 cgcaggaaga gatccaccgg cccctgatac gcgggggtgt atcccagcgg ccagcgatgt
    14041 gcgccaacgt ggaactgccc gccgcgaatg gttccctgca ggcggtttac ctcgcccata
    14101 aactccagca caaaacgagt cgccggttca cgccatacct gatccggtgc gtcagcttgt
    14161 tcaatattgc cctgactcat caccactaca cgatcggcta cttcggtcgc ctcttcctgg
    14221 tcgtgggtca cgaacacgct ggtgaatttc agttcttcat gcagttgacg cagccagcga
    14281 cgcagctctt tacgcacctg cgcatccagc gcgccaaacg gttcatcaag cagcaaaatt
    14341 tgcggttcca cagccagcgc gcgtgcaagc gccacacgct gtttctggcc gccggaaagc
    14401 tgcgccggat aacgatccgc cagatgcgcg agctgcacca tctccagcaa ttttgtcact
    14461 ttcgctttga tcgccgccgc attcgggcgt tcacgccgtg gcaacaccgt caggccaaaa
    14521 gcgatattgt caaacaccgt catatggcga aacagcgcgt aatgctggaa cacgaaaccg
    14581 actttacggt cccgcgcgtg cagacggctc acgtcggtac cgtgaaagcg aatatgcccg
    14641 ctggtttgat gctccagccc ggcaataatg cgcagcagcg tggtttttcc ggaaccggac
    14701 ggccccagca acgcgaccat ctgaccagaa ggaatatcca gtgagatatc gttcagcacc
    14761 tgggtgcgac caaacgactt cttaatattg gcaatctcaa tgctcatgat gttcctcctg
    14821 ctgcgcgcgt ttttcctggt tctccaggca ccactgcaac atactcttta aaaacagggt
    14881 gataatcgcc atcagcgtca acagcgccgc ggcggtaaag gagccgacgg tgttgtagtc
    14941 ctgctccagc aattcaatct gtaacggcag cgaaagtgtt tcgccgcgaa tcgagccaga
    15001 aaccaccgac accgcgccaa actcgccaat cgcgcgggcg ttggtcagca ccacgccgta
    15061 aagcagcgcc cagcggatgt tcggcagcgt gacgcgacgg aacatctgcc agccggacgc
    15121 gccaagcaaa atcgccgctt cgtcttcctg gctgccctga cttaacatta ccggtaccag
    15181 ttcgcgcacc acaaacggac acgtcacgaa gatggtgacc agcaccatac ccggccagga
    15241 gaacataatt tgcagattat gttcatccag ccagccgccc agcgggccgt tagagccgta
    15301 gaacagcaga tacaccagac cagcaaccac cggcgacacg gcaaacggaa tgtccagcag
    15361 cgtcagcaat agctggcgtc cggggaagtt aaagcgcgtc actagccagg ccagcagaat
    15421 accgaacacc aggtttaccg gtacggcaat cagcgcgatc atcaccgtca gccagatggc
    15481 gtgcagcatg tccggatcgg ccagattttg taacaccggc atcagtcctt tgctgaacgc
    15541 ctgtacgaag atataaatca tcggtaccag caggatgaac gccgatacca gcatcccgat
    15601 gccaatcaga aaccatttgc cccagttaat cgggcgcgcg tcataacgct tcaattgtgt
    15661 cacttccgcc attaatgacc taccacacgc cgaccaaagc gactttgcag agtgttaatt
    15721 gagaacagca acagcagcga tgccgcgagg atcaccgagg caatcgcgct cgccgccggg
    15781 taatcaaact cctgtaagcg aacaaagatc atcagcgacg tcacttctgt tttccaggcg
    15841 atattcccgg cgataaaaat caccgcgcca aactcaccga ggctgcgggt aaacgacagc
    15901 gccacacccg ccaccagcgc cggagatagt tccggcagca ccactttgcg aaaactctgc
    15961 cagcgtgttg cgccgagcgt ttctgccgcc tcttcatatt ccgggcctaa ctcttccagc
    16021 accggctgca cggtgcgcac cacaaacgga atgctggtaa aggccatcgc caccgcaatc
    16081 cccagccagg tataggtgac tttgatatca aacttcgcca gccattcacc gtaaaaaccg
    16141 ttcacggaaa agagtgaggc cagcgttaaa ccggccactg ccgttggcag cgcaaagggt
    16201 aaatccatca gcgcatcaag caacgtgcgg ccagggaagc gataacgggt taagatccac
    16261 gccatcagca gaccgaaaac gccgttaaaa atcgatgcca caaacgccga cagcagcgtc
    16321 actttatagg ctgcgaccac ctgcgggttg gtgatcacct cccagtactg cgcccagctc
    16381 atctgggcca gttgcatcac cagcgcggag agcggcagca gcaaaatcag gcacacaaac
    16441 agcagactgg tgccgaggct taaggtaaag cccggcagca cgcgtcttga agaaactgca
    16501 aacattactt acgccccgcc gctaacagct tgtctaactc gccgccgctg gtgaagtggg
    16561 ttttcatcac ttccggccag gagccaaatt tatcttccac gcggaacagc tcggtctgcg
    16621 ggaatttatt tttcagtttg tccatgactt ccgggttatt tacgcggtag taatagtcgg
    16681 tgatgatggt ttgcgcctgc gggctgtaga gccagttcag gtaggctttt gccgcttttt
    16741 ccgtgccgtt ggcctgcaca tttttatcca cccacgccac cgggaattcc gccagaatgt
    16801 tggttttcgg aatcaccact tcaaagccct gcgcttcata ctgtttacgg atgttgttca
    16861 cttccgactc gaagctgatc agcacatcgc ccaggccgcg ctcggcaaaa gtggtggtcg
    16921 cgccacgacc gccagtatcg aacacttcaa cgtttttcag gaactgggtc ataaactgtt
    16981 cggttttggc tttatcacca ccgtcggctt tgtccgctgc gccccatgcc gcaaggtagg
    17041 tataacgcgc gttacccgac gttttcgggt tcgggaaaat cagcttcacg tcagagcgta
    17101 ccaggtcgtt ccagtcgtgg atattcttcg ggttgccctt acgcaccagg aagcccatgg
    17161 tggagtagaa cggcgagcta ttattcggca ggcgcgactg ccagtccgct gggatcagct
    17221 tgcctttatc gtgcaggatt tgtacgtcgg tcacctggtt ataagtgaca acgtcggctt
    17281 ttaaaccctg caaaattgcc agcgcctgtt ttgatgatcc ggcatgagat tgtttaatcg
    17341 tcagtttgtc gccaccgtta tcttttgccc attgttgctc aaacggcggg ttcagggcgg
    17401 caaacagctc gcgggagacg tcataagaac tgttcagcag ttccgttgcc tgtacatggc
    17461 ccgccagtag cagagaagcg accagcacga gtgagttctt tttcagtaag ttaacggcca
    17521 ttgcgcaccc ttataaattt aacgactttc taatagccat catatttata acggacgtga
    17581 aaggagtaac ggttttatat accgtttggt gatttggaag ttgaaaaggg aataagacga
    17641 aatcctcccc acaaatgcag ggaggaaaca gaggtgaatc agatgccgac gctaaccgtc
    17701 tccggcagtg tgctgccgcc atcaatcaca ttctgtgtac cggttaaata gctggattca
    17761 tccgatgcga ggaaggccgc cagttcgccg acttccagcg gatcggcgag gcgacgcatc
    17821 gggattgctt tcgccatttc agtcagcacc gattctggat cttccgggtt cgactggcgg
    17881 gcaatgcttt ccgccattgg cgtgcgaacg tatccagggc agatggcgtt aacgcgaata
    17941 ccagactgcg cgtactccac cgccagcgat ttagtcaggc caacaatcgc cgctttcgtt
    18001 aaggcgtacg ccgtttcgcc cgggtctgcc accatatcgc cagtgactga agacatcatc
    18061 acaatccggc catctttgcg ggcaatcatc tccggcagca ccgccttcgt gacgttccat
    18121 acgcctttta tattgatatc aatatggaaa tcgcgatctt catcgctcat atcaaggaaa
    18181 ctgcccagac gacaaacgcc tgcgttattc accagaatat caatgcgccc ttctttttcc
    18241 ttagcgcgct taatagctgc ggctaccgac gccgggtcac gcacatcggc gacaaccgcc
    18301 gtacagcgat gaccacggcc acacagttcg tccgccagct tttcgatctc aggggagata
    18361 tccagcaaga ttaggtttgc gccatgacgc gcaaaagttc tggcaattcc ttcgccaatt
    18421 ccctgcaatg cgcccgtaat cagtgctgtc ttgcccgtga gtttacccat tttaatgctc
    18481 tccttgttaa tacagagcgt tacactccat tccctctgaa aatacaggat aaacaaaata
    18541 ttgctgcgga tcagctcact aattcaggaa aatctcaatt tagtcgctgc tttatgcagc
    18601 acctcaaacg ttacgtcagc gcgcaatcat tttcagtgac atcacatgag taatatttgg
    18661 acttaatgat tcctttgtgt ctatcaaatc gggatatatg atcccattta tctgcaatta
    18721 tttgatcgca aatattaagg tcaccaccga tgcaacttga aaagatgatt accgaaggct
    18781 cgaacgccgc ctcggctgaa attgaccgcg tatcaacgct ggaaatgtgc cggattatca
    18841 acgatgaaga taaaaccgtg ccgcttgccg ttgagcgcgt actgccggat atcgccgcgg
    18901 cgatcgatgt tatccacgcc caggtaagcg gcggcggccg tctgatttat ctcggtgcgg
    18961 gaacatccgg tcgtctgggg attctggatg ccagcgaatg tccgccgacc tacggcgtga
    19021 aaccgggcct ggtggttggt ttgattgctg gcggcgaata tgccattcag cacgcggtgg
    19081 aaggcgcaga agatagccgg gaaggtggtg ttaatgatct gaaaaatatc ggcttaaccg
    19141 cgcaggatgt ggtggttggt attgcggcca gcggacgcac gccgtatgtg attgccgggc
    19201 tggaatacgc gcgccaactc ggctgccgta cagtgggcat ttcctgtaat ccggggagtg
    19261 ccgtttcatc caccgcagag tttgctatta cgccggtagt tggtgccgaa gttgttaccg
    19321 gttcttcgcg aatgaaagca ggcacggcgc agaaactggt gctcaacatg ctctccaccg
    19381 ggctgatgat taaatccggc aaagtgttcg gcaacctgat ggtcgatgtg gtcgccacca
    19441 acgaaaaact gcatgtgcgc caggtcaata tcgtcaaaaa cgctaccgga tgtaatgccg
    19501 aacaagcgga agcggcgtta attgcttgcg agcgcaactg taaaacggcc attgtgatgg
    19561 tactgaaaaa tctcgatgcc gatgaagcta aaaaatgcct ggatcaacac ggcggtttta
    19621 ttcgcaaggc tctggaaaag gaataaccca tggctaaaga gatcagcagt gaacttctga
    19681 acaccattct tacccgggtc ggcggaccgg gaaatatcgc cagttgtggt aactgtatga
    19741 cgcgcctgcg tctgggtgta catgacagtt cattggttga tcccgatatc aaaacgctgg
    19801 aaggcgtgaa gggcgtcatt ttgaccagcg atcaggtgca ggtggttttt gggccaggca
    19861 aagcgcatcg tgcagcaaaa gccatgagcg agctgctggg cgaggcaccg gtacaggatg
    19921 ccgcagaaat cgccgcccag aataaacgcc agttaaaagc cagacaaact tccggcgtgc
    19981 aacaatttct cgccaaattc gccaccatct tcacgccgct gatccccggt tttattgccg
    20041 ccggtctgct gctggggata gcgacgttaa ttgccacggt gatgcacgtt ccggcagatg
    20101 ctcagggcac gctacccgat gcgctgaatt ttatgaaggt gttcagcaaa ggcctgttca
    20161 ccttcctggt aattctggtg ggctataacg cggcccaggc atttggtggc acgggcgtaa
    20221 atggcgcaat tatcgctgcg ctctttttgc ttggttataa cccaactgca accacgggtt
    20281 actacgccgg atttcacgat ttctttggtc tgcccatcga tccgcgcggc aatattatcg
    20341 gcgtgttgat cgccgcgtgg gcctgcgccc gcatcgaagg catggtgcgc cgctttatgc
    20401 ccgacgatct cgacatgctg ctgacctcgt taatcaccct gctgatcacc gctacgcttg
    20461 cgtacctgat cattatgccg ctgggcggct ggctgtttga aggcatgtcg tggctgttta
    20521 tgcacctgaa cagtaatccg ctcggttgtg cggttttagc cgggctgttc ctgatcgccg
    20581 tggtatttgg cgtgcatcag ggctttattc ctgtttatct ggcgttaatg gacagccagg
    20641 gattcaacag cttatttccc atcctttcaa tggcaggcgc aggccaggtg ggcgcggcgc
    20701 tggcgctcta ctggcgggcg caaccgcaca gtgcattacg cagtcaggtc cgcggggcga
    20761 ttattcccgg tctgcttggc gttggcgaac cgctgattta cggtgtcacc ctgccccgca
    20821 tgaaaccatt tgttaccgcc tgtttaggcg gcgcggcggg cggtttgttt atcgggctga
    20881 tagcctggtg gggtctgccg atgggcttaa acagcgcctt tgggccgtct ggtctggtgg
    20941 cgctgccgct gatgacttcc gcgcaaggca tccttccggc aatggcggtt tacgctggcg
    21001 gtattctggt ggcatgggtt agtgggttta ttttcaccac actctttggc tgccgtaacg
    21061 tcaatctgga ctgattatga aacggacaat gctctatctt tctctgctgg ctattagctg
    21121 tagcgtcagc gccacaaaat accctgttct gacagaaagt agttgatcaa gtgtatgcgg
    21181 cgttaaagca gtaataaaaa ggccagtcag ttgtgttact gcctggcctt taatcaatga
    21241 atcagaaacg cttacagcgc catcaacttg tccagcgacg gcgcgaagta atagccgcca
    21301 gtgaccggtt tggtgaaacg cagcatcgca tcacgcttac catcggtatc gccaaacatg
    21361 ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg
    21421 tgagtgccac tggcggtgcc gtacggcagg ctctgacgaa caatcttcag ccctttgcca
    21481 tcttctttca gatcaacgcg ggtcaggtga gaggtttccg gacgttcgtc gccgtcaatc
    21541 tcttcgttgg cctctttggt gcgcccgatc atcatctctt gatcgtgaac gctcatccga
    21601 ttgagctgtt tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc gcccgcatcc
    21661 acgccgtctt tgataacagc cacttcgcgg cgcgtctctt cacccgccgg gttttctgta
    21721 ccgtcaacaa agccgctcag gtcacgctct tcaacccaac ggaagccgtg gatctcttct
    21781 ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct gagcgacaga gaagttcacg
    21841 tcgtgacgca gagaaagaat gtggatcaac acatcgaact gggtcgtcgg tgcaagacct
    21901 ttaccgtagc ccggaaaatc tttcagctct tcggccccaa cgccgccgct cagagcgcgc
    21961 caggtattgt taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca
    22021 aaagttgcca gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc aacttcccct
    22081 ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat gccactctga
    22141 acctgagaca ttgttcctcc tgaaaataat aatgccacgc aatccgtggc attgttgggc
    22201 gctattttac gtgttttttc gcggcggcgg tttgttcaga cgcaaattaa cgccgccaga
    22261 taattttact gactttccag tttttcaggg tatcgtccga aggcattaac ccttcagggc
    22321 cactccattc cccgctgaac tggtagctaa tatgttgact gccttcggct ttacattcga
    22381 cggcacgatt atcatcactg tcggcctttt ggcaattgcc aaatgctttg ctgtaaaggt
    22441 cactaaacgg tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta tccagcacat
    22501 caatgcggct gatcgtgcct tgatcaccgt taatcaccat cgccacgttg tcgcctttca
    22561 tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt cattccgctg cgcaggcgat
    22621 aatcgccatc aagcgcatca gcaatggctt gttcttgcag tggtgtggac gccgttaatt
    22681 cacccacgcc ctgctcgctc actttggtgg atgatccaaa ccagttccac gggttagcgg
    22741 ccgaccagtt aactgacgac atcgtggaac agccggtcag catcaacggc atagcgcata
    22801 acattaaacg cagcgatttc atgtcacttc ctttggttat tcaataacgt tgattggagt
    22861 gcaaattcac caaaaagtgc cgttattctt tttcttcatt aaaacaagcg cgtaaacgtc
    22921 gattggtcag cagccagatt aacgccacga tatccgccac taccagcgcc agaccgatac
    22981 cgctaacgga ttcaccgttc agccacagcc acggttgcca acaaagtaaa accacttgcg
    23041 ccaacagcaa cagaaaatag agcacatgcc agatacgggg aaatgaagcc cgccgaccac
    23101 tcagcaaaaa tgccagcacc gccggaatac cgggaatcaa ccccagccag aaattatcgt
    23161 gatcgggata aaacagattt agcagcgcag taccctgctc gcgcgacgct ccggcaatga
    23221 caaacagcac ccaggttcgc gcctgaagca atagcacaag ccagaagagc aagggtaaac
    23281 gcaggcgacc gtgcgcatca taatggacag gatgaaactc agtactcttc atcttcaatc
    23341 aaacgcttac ccagactcag cacgtcggcg tgttcatatc ccaggcgttc atacattccg
    23401 agcaccatgt cgttatcttc cggcacgttg atctgaattt tcggacagcc gcgagcaatc
    23461 agctttttct ccaaccgatt aagcaacgca ttagcaatcc cacgcccacg aaactcggga
    23521 tgcacgccaa gataataagc agacccgcga tgcccgtcat aaccgcccat caccgttccg
    23581 accacttcac cgttcacctc agcgaccaga aacaaactga tgtcatggtt catcttacgc
    23641 tcaatgtcca tttccggatc gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg
    23701 atgacctctt cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt cgttaccttt
    23761 ttgcgggtta aaaggctgat tatggcgtga acggtcgaat tagccaatat ctgacgaaaa
    23821 tcggttgaaa aagtggcata atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat
    23881 tctcaacagc aaaccgtcgt aacggattac gcgatacgat ataacatctg gaactttatt
    23941 attacaactc aggccgtatg agcactttta aaccactaaa aacactcact tcgcgccgcc
    24001 aggtgctgaa agccggtttg gctgccctga cgttgtcagg aatgtcgcaa gccattgcca
    24061 aagaagaacc gttaaaaacc agtaacggac acagcaagcc gaaagcaaaa aaaagcggcg
    24121 gcagacgtgt cgttgttctc gatcctggcc acggcgggat tgataccgga gcgatcggac
    24181 gcaacggttc gaaagaaaaa catgtggtgc tggcaattgc caagaatgtc cgttccatat
    24241 tgcgtaatca tggcattgat gcgcgtttaa cgcgttctgg cgatacgttt atcccactgt
    24301 acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct gtttatgtca attcatgccg
    24361 acggctttac caacccgaaa gctgccggtg cttcggtatt tgccctctct aaccgtgggg
    24421 caagtagcgc aatggcaaaa tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg
    24481 gtaaaaaagc gactgacaag gatcacctat tgcagcaagt gctgtttgat ctggtgcaaa
    24541 ccgatactat taaaaacagc ctgacgctcg gctcgcatat tctgaaaaag attaagccgg
    24601 tgcataaact gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt
    24661 cggttccgtc ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa gagcggctgt
    24721 taggcactgc agcgtttcgc cagaaaatcg ccacagcgat tgccgaaggc gtgatcagtt
    24781 atttccactg gtttgacaac cagaaagcac actcgagaaa gcggtaagtg atgaaacccg
    24841 acgcacacca ggttaaacag tttctgctca accttcagga tacgatttgt cagcagttga
    24901 gcgccgtcga tggcgcagaa tttgtcgaag atagctggca gcgcgaagct ggcggcggcg
    24961 ggcgtagtcg ggttttgcgt aatggtggtg ttttcgaaca ggcaggcgtc aacttttcac
    25021 atgtccacgg tgaggcgatg cctgcttccg ccaccgctca tcgtccggaa cttgccgggc
    25081 gcagtttcga ggcgatgggc gtttcgctgg tggtacatcc gcataacccg tatgtcccca
    25141 ccagccacgc gaatgtgcgc ttttttattg ccgaaaaacc gggtgcagag cccgtctggt
    25201 ggtttggcgg cggcttcgat ttaacccctt tctatggttt tgaagaagac gccattcact
    25261 ggcaccgcac cgcccgtgac ctgtgcctgc catttggcga agacgtttat ccgcgttaca
    25321 aaaagtggtg cgacgaatac ttctacctca aacatcgtaa cgaacagcgc ggcattggcg
    25381 ggctgttctt tgatgatctg aacacgccag atttcgacca ctgttttgcc tttatgcagg
    25441 cggtaggcaa aggctacacc gacgcttatt tgccaattgt agagcgacga aaagcgatgg
    25501 cctacggcga gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat gtcgagttca
    25561 atctggtctg ggatcgcggc acgctgtttg gcctgcaaac tggcgggcgc accgagtcga
    25621 tcctgatgtc aatgccgcca ctggtacgct gggagtatga ttatcagcca aaagatggca
    25681 gcccagaagc ggctttaagt gagtttatta aggtcaggga ttgggtgtaa ctccctcacc
    25741 cccactcccg catccgctga tgcagcgtca gtgacggctt ctcggcaaac agttgctgat
    25801 aatccgtggc aaattgccca agatgccaga atccccactg catggcggcg tcttttaccg
    25861 ttgtgctttg tgaccacgga cttatgagtt cgcggcgcac ggcgttcagg cgaatacgtt
    25921 tcagccacgc gttcggacca atgcccaaaa tggcgtgaaa tgcgttttgt aatgtgcggc
    25981 ggctgacgtg caattgattg cacaagtcca gcaccgtcac cggttcggac atgttttcca
    26041 gcacatattc acgggcgcgg gaaagcaacc gacggtaact ctgatgactg atgctttccg
    26101 ccgtcaccat tggctgcgct tcttccagca tcgcccccat cgccattagc aaattatccc
    26161 ccagcacttt acgcacagca ggctgatgaa gattttccgg gttctcgcaa aatgtcgcca
    26221 gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg ctctttcact tccaacgccg
    26281 actggctacg cagcatatgt aatacccgat ccggattatg caaaaagtta gcctgacggg
    26341 tgatgacatc ttcagaaagc actacgccaa ggatggtgta atcgtccggc gtacttaatt
    26401 caaactcggt tccaccaggg cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga
    26461 taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca gaacgaattc ggccagacca
    26521 ggcacgactg acgcagcgcc agaccggtgt attcacgaaa aacctgaata tcatcgagta
    26581 gaatttccgt aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa atctgctgcc
    26641 aggcggtaat tgtcaaagca tgttcataga cgtccgttgt ctgtcgttga tgaacattat
    26701 ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa gggttcatga taaagatggt
    26761 gcaaattggc tgtacgggtc tttttcatga tgttaatgcc gggagttgta ggacacccgg
    26821 cacctccgac aggttaatgg ggcttgagac ggtaacgact actgcgttta cgtaacgtcc
    26881 cggcagaaaa gagctgttcg agcgcatttc tggctttttc cagcggccag ccaaagtggg
    26941 cagccacttc tcctgccgtc attccctgac gtactgatgt taacagcgcc aacaactcgt
    27001 cagcagattc cgcgacgata actggcgcgt cgggttcctt tacgggttgc ttcggctggc
    27061 ggttaaagcc agtaaccagc cactgggtgt catcgtctgg ccgaccgatc tccttacggc
    27121 tgatgacacg cccggtacgc atcgcggcag cacaacctgc gtccagcgcc gcacgacacg
    27181 ccgccagatc gccttccacc accagcgtta ggcgaccagg gtcaagcact tcgtgactga
    27241 gcagacgaac gttagctgct ttgagcatgg catcagttgc atctatcgcg gcgaccatgc
    27301 cgtccacttc cagcaatccc agtgcattga tcatcggcaa cctccgttac gcacgctgga
    27361 ttggattacg cgcgatttcc agcactgcat cggtaaaggc gttacaggct gctttacacg
    27421 cggcctggct accggttaaa aatgctgccg agtagttggt ttcagacggt ggcgggacat
    27481 aggtcaccag ctgcacgtcg gcagatttca acgctgcatc aatgccgtag gtcgcttcca
    27541 gcggcggtgc caccagatac gccatcggat cgccaagcgt gatcccggcg gttgatgaga
    27601 gataagaacc ggtacgcgaa actacatgtg ccaggaaggc tgtgttttct gcgtcattag
    27661 cccactggaa agcagcgcca ttttcaatat ttgcaaccat cgcatccaga ccagcacgca
    27721 cttccgccgg gtttggtcca ccaagcataa tcagcacctc accggcagtc ggtgacgggc
    27781 cgtgtgcagc gcccgcatac agcgagcgac catataccac ttcgaccatc gcctgcttgg
    27841 tcgcttcgtc agccgcaatg taggtgacgt catctgaatc agcagaaatc agcccgaggc
    27901 tacgaatatg cggcggcaat ttcagttcac gtgcaaattc ggcgttaacg gaggcaatca
    27961 cccgcatggc ggttaccgac ggtcgaatca aatctaaagc tggcatgatg cctccttatc
    28021 gggtcatgtt gatgccggat gctttctgct ccagcatacg tttcgccaaa tccacaatga
    28081 cggctgcggc ttcaaccggc ggcgtgccgc cctggtgaat gttagagata caggtgcgat
    28141 cggcctcaac ggtggtcgcc atacgcggcg agtaaacggc gtagcaggag aggctttctg
    28201 actgccccaa ccccggacgt tcgcccacca gcaggatcac tactttcgcg ccgaggatct
    28261 caccgatctg atcttcaatc ttcacgcgac cataacgtac aaagaatggc gtaccgactt
    28321 tcagcccggc ttgcttcagg cccgccatca gcggcggcag gatctcttcg tagttcacgg
    28381 tgatcgcatc agttgacagg ccatcagaaa taacgacctg cacgtccgga ttagcaacac
    28441 actgtgcttt cagcgcttca acagcttctg cacacaggcg gcggcccata tccggacgag
    28501 tcaggtagag atttttgtcg ctgatttcgg agcggacttc cagcaagccc tgcgctttca
    28561 cccactcttc cggtacttct ttcagtacgg tatctttcga acgagagtga tcggccagga
    28621 aacgcagcaa cgcctgggta cgcggacgcg gaccggcacg accggtacaa acgcgggcca
    28681 cggtgctgcg acgcagttct gttaatacgt ccgcgcgatg tgggttttcc acaccaatcc
    28741 acgcttttgc ttccgcagaa cctaaatcca gcgcgcagct ttccgaggtc accggtgccg
    28801 cacagttggt ggtggcgcac tttgcttctg acggcgcggg ggccgtttgt cccattgacg
    28861 ccatcacact gcgtacaatt tcttcaatct gtttttgatc catgatgtgt tatctccgcg
    28921 tcatcagaag aacagtgacg gatcgcccgc ccgtttggtc aggcgaccgt ttgccataat
    28981 gcccatgctt tccagccagc gttcaaactc cggtgacggg cgcaggttga gtaactgacg
    29041 cacagtggca gtgtcgtgga atgcggtggt ctgatagttg agcatgatgt catcacccag
    29101 cggcatcccc atgatgtagt tgcagcctgc ggtggcgagc aggatcatca ggttttcgtt
    29161 gaggttctgg tcagcgtcag cgtggttggt gtagcagcag tcacagccca tagagatgcc
    29221 gctcagcttg cccataaagt gatcttctaa gcccgcacgg ataatctggc ggtcgttgta
    29281 gagatactcc ggcccaataa aaccgaccac ggtgttgacg ataaacggat cgtaatgacg
    29341 cgccagccca tagttacgcg cttccatcgt cacctggtct gcgccgaagt tagcgccagc
    29401 ggacagcgcc gaaccttgtc cggtttcgaa gtagaggcag ttttccccgg cgatacggtt
    29461 aaactctgca cccactgcgc gcgcttcgtc gagcatcgcc agctccacgc caaactcttt
    29521 cagccctttt tcgctgccgc aaatactctg gaaaatcagc ccgcccggtg cgccgcgacg
    29581 aatcgcttcg atctgggtgg tgacgtgcgc cagtacgcag ccctgagttg ggatgttgaa
    29641 tttgtcgatc acgccataga tggtatccaa cacgcggctt aagttttcca cgtcgtcagt
    29701 caccgggtta acgccgatca ccgcatcgcc caccccgaag gaaagccctt cgtagatttg
    29761 cgcggcgata ctttgcacat cgtcacgggt atcgtttggc tgcaagcggg cgctaaaggt
    29821 gcccggaata ccgatggtgg tattggcctt tttgatcacc ggcattttct tcgcgccgta
    29881 gatcaggtcc gcgttggagc aaatcttcgc taccgccgcg accacttccg aggtcagccc
    29941 tttgcgggta aaggcaatgt cgtccacgct ggtttcatcg ctcagcacat actcacgcag
    30001 ttcgctgatg ctccagtttt taatctggtt gtaggccgtt tcgttaacgt cgtcctgaat
    30061 cagccgcgtc acgcagtcat cttcataggc aatcaccgga ttattgcgga tgtccgctac
    30121 ggtcatttcc gacaacacct gctttgccgc cacgcgctcc tgtgagcttg ccgcagcaac
    30181 gcccgccagc acatcccccg aacgcagttc gttggcttta gccagcacct cttttacatc
    30241 cttaaactga tatacattgc cgaacaatgt ggtctttagt ttcataagtc gttccctcag
    30301 gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg atccgccaaa aagaggcgta
    30361 ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt caatgactgc caacgggagt
    30421 tgttgtagct gtgggcgcaa caacatgccc agagctttac caaagtcctg cccggccacc
    30481 accagcaggg gatgcggatt cggaaaacgc gcgacgaaat cgaccagcgc gttgatgacc
    30541 gtcagtaccg cggcgtaacg cacaggcagc gaggcgggaa gcgccagcac atacgcgtca
    30601 gttttggggc aaagatccag ttgaatcagc gcctgttgcc aggcactgac cagatccgtt
    30661 tcatcaatcg ggatcgccac cggcaaattg cgcagcggca gttgtacgcc ctccagccag
    30721 attgtgctgc cagagagcga aagggtatga gcacccgcgc caatcaccgt ggcgcgcacg
    30781 gtttgcgccg gaaactgcac gttcatctca cgcagacgcg gatggtcatg cagcgccgtt
    30841 gccagaagcg ggcctatatc ggcaaaacag aacgggtcgg cgggctggtg gcgataacat
    30901 tcgcccacgc cgccagaaag cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg
    30961 gtttgcatca atgcctgcgc gagcggcgag agcgttccgt caatcacttc gacaatcagc
    31021 tctgccatcc ggcgagtaac ctgcaccagc tgcgcgccgg tcagcgaacg ggcgtcagta
    31081 ccggcaccga agcactcatc cacaatcatc tgccccggtt tatgagcgta aaccacgcgc
    31141 ccctggctgt cggtttccag caggcgaccg ccgacgttga ggcaggcagt gccgctgatt
    31201 tttccggcat cgaacagggc gtagttcgcg gtgccaccgc cgatatcgat attcaggacc
    31261 cgacacagcc gttgttcaga aagggtttgc gccccggcac cgtgtccggc gatcacggat
    31321 tcaagatgcg gcccggcgct ggcgacgaca aaatcgccca gcgaccggga gagcgccatc
    31381 accgccgggc gagcgttgcg ggtttttgcg ctttcaccgg tgataatgat ggcaccagaa
    31441 tcaacgcttt ccggcgcaat gcccgcagcc tgatattgct cgaggattaa ggttttcagt
    31501 tccgcttctt ttaaaccacc ctgtttatcg acaggggtaa aaaacaccgg gctttgccag
    31561 ctaatttcgc gtttgatgaa ttcgtagcgc ggcacctgcg acaccgccgc acggttaacc
    31621 agctccaggt gggagaagat cacctgggtg gtggtggtgc cgatatcgat accgacgctc
    31681 agtagctggc gagtgttcac gattgcgcct ccgcttcagt tttagccgcg gtcgcgtctt
    31741 cttttggcac cagcatcatc gccacgccaa ttgccgttac gccgccgatc aatttgccga
    31801 caatcatcgg gaagatcatg gcgttcatgt tggcagcggc gaagcctaaa tggtcgccca
    31861 gggcgaaagc agcggaaacg gcgaaggcgc agttgatgac tttgccgcgg gtatccatct
    31921 gcttcatcat gccaaacatc ggaatgttgt tggcaagcgt tgccaccatg ccggctgccg
    31981 cgatattgtt catattcagc acgttaccga cactcatcag cggtttttca aaccagcgag
    32041 tcagcagcag taccatcgga tacgccccta acagaacgca ggagatcgag ccgataactt
    32101 caatggcgcg catcacttca ccgggtttat cgccaggggc cataaagata ggatcaagac
    32161 ccgggatcag ttcccagcca aggaggaatt tcactaccgc agcggcaaga ccgagggtga
    32221 tcaatgcaac gaggaatttg gcgaagatct ggaagccgtt gatcattttt tccgggatga
    32281 atttcagtcc cagcgccacc agcaccgcaa cgataagtac cgggatcatg ttcatcagga
    32341 tcagcgcaaa ggtgaattca acaggctggc cgttgatctg cacaccggag tacatcgcta
    32401 ccagaccacc agcgatacag ccaatcggaa tggtcactat gcccgccagc acgccgagcg
    32461 ccagataacg acggtcagaa ggttcgataa tgccgagcgc cactggaatg gaaaacacaa
    32521 tcgttggccc catcatcgac ccgagaatta acccagagta tagccacgct gctacgtcac
    32581 cgcccgccag ctctttggcg aggaagaagc cgcccatatc gcacgccagc agtgttccgg
    32641 cgaacattga cgggtttgcg ccgagcattt cgtaaaccgg gataatcacc ggtccgagaa
    32701 cgtgggccag taccggtgcc agtgcggtca taccgaccat cgccaggccc agagcgccca
    32761 ttgccataaa gccttcttcg aactgaccgc cggatccttc gatactttta ccgaacttac
    32821 cgaggaaacg agcagaaccg ccgaactgcg acaggatcct gtctacggca gctatcagca
    32881 taaagaacat catgatgtac atgatgattt cgttaattcc catcgccttt actccctgtt
    32941 agttgttatt tattggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
    33001 gaaggcc
//
LOCUS       DAZKHK010000048        32320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKHK010000048 DAZKHK010000000
VERSION     DAZKHK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.048"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..3025
                     /locus_tag="ACHOS5_002245"
     CDS             <1..3025
                     /locus_tag="ACHOS5_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFP9777059.1"
                     /translation="VFLSSGISPDYLQQYSSFELRLRKHLSLAEESLWQKCAQKLIAA
                     IPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGRKKLPSLEWLKIVATDEHILASLEKY
                     HEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPYAASDYCADVLRHINHPFALTLLI
                     RVAGHTKRCHDRMTKACAAFPHAALAALAELLVQKEENSWRIMLMTMLISQPTLAEQV
                     IPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIVVSPPWLSKKKKSPIPVLDLAPLN
                     LESICTITDTEAKEFQTHWDWEPHKPGEGAKDFLYSLGYRRWDFDTYKYIGASDSAID
                     AWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPMQKAIKLWEFLSKEPHTAIKPVML
                     YLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAPAVARAFNKLKTLRQDASSWLLKY
                     PQHAITGLLPAALGKTGEAQDNARAALRMLTENGHQPLLQEIARRYNQPEVTDAVNAL
                     LALDPLDNHPTKIPTLPTFYQPSLWTRPLLKANAQSLPDSALLHLGEMLRFPQEEALY
                     PGLLQVKDACTTDSLAEFAWDLFTAWQTAGAPSKESWAFTALGVLGNDDTARKLTPLI
                     RAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIAESR
                     ELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFVRDVSGSRLKDLPKP
                     NKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRWSPENFQLFLVEHPL
                     VRHLTRSLIWGVYSAENQLLACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLEISP
                     TDAAAFGQLFADYELLPPFRQLDRNSYALTEAERNASELTRWAGRKCPSGRVMGLANK
                     GWMRGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWEGKA
                     ESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            3035..6667
                     /gene="yehI"
                     /locus_tag="ACHOS5_002246"
     CDS             3035..6667
                     /gene="yehI"
                     /locus_tag="ACHOS5_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP9777060.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITENIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLSTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGLGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPAMWSRPRLIKNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIYDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDASQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDESIDLPADALIGIAHPLEMAAEMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESASNSLTRWEGKSASVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            6728..7044
                     /gene="yehK"
                     /locus_tag="ACHOS5_002247"
                     /pseudo
     CDS             6728..7044
                     /gene="yehK"
                     /locus_tag="ACHOS5_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
     gene            7676..8764
                     /gene="yehL"
                     /locus_tag="ACHOS5_002248"
     CDS             7676..8764
                     /gene="yehL"
                     /locus_tag="ACHOS5_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP9777061.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKKNDHAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8775..11054
                     /gene="yehM"
                     /locus_tag="ACHOS5_002249"
     CDS             8775..11054
                     /gene="yehM"
                     /locus_tag="ACHOS5_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFP9777062.1"
                     /translation="MSEPLIVGIRHHSPACARLAKSLIESQRPRYVLIEGPADFNDRI
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRDTRMDNSDTLWDHLFEDESQQTALPSALARYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMLQNNGDVLVVCGGWHAPALAKMWREYTQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWGWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGAIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLSLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWQLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPTQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALRMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11047..12183
                     /gene="yehP"
                     /locus_tag="ACHOS5_002250"
     CDS             11047..12183
                     /gene="yehP"
                     /locus_tag="ACHOS5_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9777063.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12180..14183
                     /gene="yehQ"
                     /locus_tag="ACHOS5_002251"
     CDS             12180..14183
                     /gene="yehQ"
                     /locus_tag="ACHOS5_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9777064.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GAIIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDSAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIISQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPINQVDNLFILPIAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPPTAHQLLMRCQALLIQLLHNGWRYQEQSAIGEAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            14308..14769
                     /gene="yehR"
                     /locus_tag="ACHOS5_002252"
     CDS             14308..14769
                     /gene="yehR"
                     /locus_tag="ACHOS5_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP9777065.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(14810..15280)
                     /gene="yehS"
                     /locus_tag="ACHOS5_002253"
     CDS             complement(14810..15280)
                     /gene="yehS"
                     /locus_tag="ACHOS5_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9777066.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(15327..16046)
                     /gene="btsR"
                     /locus_tag="ACHOS5_002254"
                     /gene_synonym="yehT"
     CDS             complement(15327..16046)
                     /gene="btsR"
                     /locus_tag="ACHOS5_002254"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP9777067.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(16043..17728)
                     /gene="btsS"
                     /locus_tag="ACHOS5_002255"
     CDS             complement(16043..17728)
                     /gene="btsS"
                     /locus_tag="ACHOS5_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP9777068.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGQVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            17950..18681
                     /gene="mlrA"
                     /locus_tag="ACHOS5_002256"
     CDS             17950..18681
                     /gene="mlrA"
                     /locus_tag="ACHOS5_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP9777069.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            18741..18848
                     /gene="yohO"
                     /locus_tag="ACHOS5_002257"
     CDS             18741..18848
                     /gene="yohO"
                     /locus_tag="ACHOS5_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP9777070.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(18829..19560)
                     /gene="yehW"
                     /locus_tag="ACHOS5_002258"
     CDS             complement(18829..19560)
                     /gene="yehW"
                     /locus_tag="ACHOS5_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP9777071.1"
                     /translation="MKMLRDQLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(19565..20491)
                     /gene="yehX"
                     /locus_tag="ACHOS5_002259"
     CDS             complement(19565..20491)
                     /gene="yehX"
                     /locus_tag="ACHOS5_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP9777072.1"
                     /translation="MIEFSHVSKLFGTQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGHSELGVRLLSLRSVADYVRREEWAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            complement(20484..21641)
                     /gene="yehY"
                     /locus_tag="ACHOS5_002260"
     CDS             complement(20484..21641)
                     /gene="yehY"
                     /locus_tag="ACHOS5_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP9777073.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(21648..22565)
                     /gene="osmF"
                     /locus_tag="ACHOS5_002261"
     CDS             complement(21648..22565)
                     /gene="osmF"
                     /locus_tag="ACHOS5_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP9777074.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLRPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(22755..25052)
                     /gene="bglX"
                     /locus_tag="ACHOS5_002262"
     CDS             complement(22755..25052)
                     /gene="bglX"
                     /locus_tag="ACHOS5_002262"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP9777075.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            25248..26963
                     /gene="dld"
                     /locus_tag="ACHOS5_002263"
     CDS             25248..26963
                     /gene="dld"
                     /locus_tag="ACHOS5_002263"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9777076.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(27002..27934)
                     /gene="pbpG"
                     /locus_tag="ACHOS5_002264"
     CDS             complement(27002..27934)
                     /gene="pbpG"
                     /locus_tag="ACHOS5_002264"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP9777077.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(28108..28695)
                     /gene="yohC"
                     /locus_tag="ACHOS5_002265"
     CDS             complement(28108..28695)
                     /gene="yohC"
                     /locus_tag="ACHOS5_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP9777078.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            28865..29443
                     /gene="yohD"
                     /locus_tag="ACHOS5_002266"
     CDS             28865..29443
                     /gene="yohD"
                     /locus_tag="ACHOS5_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9777079.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWSLIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(29563..30324)
                     /gene="yohF"
                     /locus_tag="ACHOS5_002267"
     CDS             complement(29563..30324)
                     /gene="yohF"
                     /locus_tag="ACHOS5_002267"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9777080.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(30377..31813)
                     /gene="mdtQ"
                     /locus_tag="ACHOS5_002268"
     CDS             complement(30377..31813)
                     /gene="mdtQ"
                     /locus_tag="ACHOS5_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP9777081.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCATMHETRQALSQQTPAAQVDTV
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQSAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLNTLTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVAQAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEAKIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            32037..32120
                     /gene="yohP"
                     /locus_tag="ACHOS5_002269"
     CDS             32037..32120
                     /gene="yohP"
                     /locus_tag="ACHOS5_002269"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP9777082.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         7901 a         8697 c         8163 g         7559 t
ORIGIN      
        1 tgttttcctg tcgtctggca tttcacctga ttacttgcag caatattcca gctttgaatt
       61 acgactacgt aaacatttat cactggcgga agagtctctc tggcaaaaat gtgcacaaaa
      121 acttattgcc gcaattccac atattccaga atggcggcaa ccattaattg ctttgttatt
      181 acccgaaaaa ccagaaattg cacatgaaat agcccagcgt ctactggggc gaaagaaatt
      241 accctcgctt gagtggctaa aaatagtggc gactgatgag cacattcttg cctcattaga
      301 aaaatatcac gagccatatg ccatttttga tgattactat tgtggtgcga tatggtcagc
      361 caccgtatta caggagcaag gtgttgcagc cctgccccga tttgctccct atgccgcaag
      421 tgactactgc gccgatgtgt tgcgtcatat caatcatccg ttcgcattaa cactgcttat
      481 acgtgtagcc gggcatacta aacgctgtca cgatcgaatg acgaaagcct gcgctgcgtt
      541 cccacacgca gcactggcag cactggcgga acttctagtg caaaaagaag agaatagttg
      601 gcgcattatg ctaatgacga tgcttatctc gcaaccgaca ctagcagaac aggtcattcc
      661 ctggctttcg acacccgcag ttgcagtgct gaaatcatgc cagcaacaac tgacacagcc
      721 ctcaaaccat gccagcgccg atctactgcc ggccatagta gtctccccac catggctttc
      781 gaaaaagaaa aaatcgccga ttccggtgct tgatttagca ccgctcaacc tcgaatccat
      841 atgcacaata accgacacag aagctaaaga gtttcaaact cattgggact gggaaccgca
      901 taagccaggt gaaggtgcta aagatttcct ttactcgctg gggtatcgcc gctgggattt
      961 tgacacatat aaatatatcg gcgcttctga ctcggcaatt gacgcatggg aacgtgagga
     1021 cttcgctaca ctcatccaaa tgtttaaagc ccaccatgca ccgtatcagg gggagtggca
     1081 tctcaactca cttccctttt tacctatgca aaaagcgatt aagctatggg aatttctcag
     1141 caaagagccg cataccgcga taaaaccagt catgctttat ttgcgactgg caggtatgag
     1201 tggttttcta cactcgtttt cacgctaccc tcaagaaggt tttgcggttg ctaattattt
     1261 tgccgcaacg gaactggcac ctgccgtcgc ccgcgccttc aacaaactca aaacattacg
     1321 acaagacgcc agtagctggc tgctgaaata cccgcaacac gccattaccg gcctgctgcc
     1381 tgcagcgctc ggcaaaaccg gtgaagcaca ggacaacgcc cgtgctgcct tgcgtatgct
     1441 tactgaaaac ggtcatcagc cattgctgca agaaatcgcc cggcgttata accagccgga
     1501 agtaaccgat gcggtgaacg ctctccttgc gctcgatccc ttagataatc acccgacaaa
     1561 aatccccact cttccgacct tttatcagcc atcgctctgg acgcgcccgt tattaaaagc
     1621 aaacgcccaa tcactgcctg atagcgcact cctccacctc ggtgaaatgc tccgcttccc
     1681 tcaggaagag gcactgtatc caggattatt gcaggtgaaa gatgcctgta ccaccgactc
     1741 actggctgaa tttgcctggg atctgtttac cgcctggcag accgctggcg cgccgtcgaa
     1801 agagagttgg gcgttcactg cgttaggcgt tctcggtaac gatgacaccg cccgcaaact
     1861 gacgccatta atccgtgcct ggcctggcga atctcagcat aaacgtgcca ccgttgggtt
     1921 ggatatcctc gccgctatcg gtagtgatat cgccctgatg cagcttaacg gcatcgccca
     1981 gaaactgaaa ttcaaagcat tacaggagcg ggcgaaagag aaaattgccg acattgccga
     2041 aagtcgcgaa ctgacggtag cggagcttga agatcggtta gcaccggatc tcggtctgga
     2101 tgataacggt tcgctgctgc tggattttgg cccacggcag ttcaccgtca gctttgatga
     2161 aaccttaaaa ccgtttgtgc gcgatgtttc cggcagccgc ctgaaagact tacctaaacc
     2221 caataaaagc gatgatgaat cacaggcgaa cgatgcggtt aaccgctaca aattgctgaa
     2281 aaaagatgcg cgtaccgtcg ccgcccagca ggtagcaagg ctggaatccg ccatgtgcct
     2341 gcgtcgccgc tggtcaccgg aaaactttca gctcttcctg gttgagcatc cgctggtccg
     2401 ccacttaacc cgcagtctga tttggggcgt ttatagcgcc gaaaaccagc tactggcttg
     2461 ctttcgcgta gcggaagata acagctacag caccgctgac gatgatcttt tcaccctgcc
     2521 ggaaggcgat atctctattg gtattcctca cgttctggaa atatcaccga cggatgctgc
     2581 cgccttcggg cagctttttg ccgactacga actgttacca ccgttccgcc agcttgatcg
     2641 taacagttac gcgctgacag aagccgagcg caatgccagc gaactgacac gctgggcagg
     2701 cagaaaatgc ccgagtggtc gggtaatggg gctggcaaat aaaggctgga tgcgtggtga
     2761 accgcaggac ggtggctgga tcggctggat gatcaaacct ctgggccgct ggtcgttaat
     2821 catggaaatc gatgaaggtt ttgcggtcgg catgtcgcca gccgaactca gtgccgagca
     2881 actcttaagc aagctgtggc tatgggaagg caaagcggaa agctatggct gggggagtaa
     2941 ttcaacacag gaagcgcagt tctccgtact cgatgccatc accgccagcg agctaattaa
     3001 cgatattgaa gccctgtttg aataaggaaa gagcatggac aaggaattac cgtggctggc
     3061 ggataacgcc caactggaac tgaaatataa aaaaggcaaa acaccactca gccatcgcaa
     3121 ttggccaggc gaaccggtac ctgttatcac tgaaaacatc attcagacac tgggtgatga
     3181 attgctacaa aaagcagaga agaaaaaaaa cattgtctgg cgttatgaaa acttttcact
     3241 ggagtggcag tccgccatca cgcaggccat caacttgatc ggcgaacaca aaccctcaat
     3301 cacggcccgg acaatggcgg cgctagcctg tatcgcgcaa aatgatagcc aacagttgct
     3361 cgacgaaatc gtccaacaag aggggctgga atatgcaact gaggtggtga ttgcacgcca
     3421 gtttattgta cggtgttatg agagtgatcc tctggtagta acattgcaat atcagaacga
     3481 ggactatggc tatggttatc gctcagaaac ctataacgaa ttcgatctcc gattgcgtaa
     3541 gcatctctct ctggcagagg aaagctgctg gcagcgttgc gccgacaaac tcattgccgc
     3601 actgccagga ataaccaaag ttcgccgccc ttttattgcg ctgaacctcc cggaaaaacc
     3661 cgaaatcgcc aacgagctgg taagccttga atgttcgcaa actcattttc gctcaaaaga
     3721 gtggttaaaa gttgttgctg atgaccctaa ggcggtgaaa gaactcgcac gttactggag
     3781 ccaggatata tttagcgatc gagaagccag ctacatgtcg catgaaaacc acttcggtta
     3841 cgcggcctgc gctgcccttt tgcgcgaaca aggactggca gccgttccgc gcctcgcgat
     3901 gtatgcacat aaagaagatt gcggcagtct gctggtacaa attaaccatc cgcaagtcat
     3961 ccgcaccttg ctactggtgg ctgataaaaa caaacccagc ctgcaacgtg tggctaaata
     4021 cagtaaaaac ttcccccatg cgacgctcgc cgcactggca gaactgctgg cgttaaaagc
     4081 cccccctgcg cgccctggtt atcccatcat cgaagaccaa aagctgcctg cacagcaaaa
     4141 agcacgtgat gaatactggc atacgctgtt acaaacgctg atggcatcgc agccacaact
     4201 ggcagaagag gtgatgccgt gcttaagtac acaagcccag gcagtggtga atggttattt
     4261 atcggcatcg ccaaaacttg ctttcgagag tactcatagc aacgacctgc cagaaatact
     4321 tgtttctcca ccgtggcgag gtaagaaaaa aacggcattg ctgcgccttg atttagtgcc
     4381 tcttgaatta gcgcccaaag cccgctggca accaggcgaa cgagagagac ttgccgccac
     4441 agagtctgcc cgttatttca gcacgggatc ttttacggaa cgcatggaac gaaaaagtgg
     4501 tcgagttgta ctacaggagc taggtcttgg ggatgatgta tggctgttcc ggaattatat
     4561 actgcccggc aaactggatg cggcgcgcaa atcattagtg ggtcagtggc attactcccc
     4621 gcgcagagta gaggaaatca acaatggctg gcactccacg gaagcaaaat cagccgaaca
     4681 ggcgcttcgt tccggcgacg ttgaagcgtt aattaacacc tgggaaaatg atagctactc
     4741 acgttatcgt ccggaaaaga gtgtctggaa cctgtattta ttggcacagt tgccgcgtga
     4801 gatggctttg actttctggc tgcgtatcaa tgagaaaaag catctgttcg cgggtgagga
     4861 ctattttctc agtatcctcg ggttggatac attacctggc ctgttattgg ctttttcaca
     4921 tcatccaaaa gaaacatttc cgttaatttt aaatttcggc gcaacagaac tggccctgcc
     4981 cgttgcccgc gtctggcgac gtttcgcggc gcagcgtgat ctggctcgcc agtggatttt
     5041 acaatggccg gaacatacgg ctactgcact tatccctctt gtctttacaa aacccagcga
     5101 taatagcgaa gctgcattac ttgccctgcg tttactgtat gaacaagggc atggcgaatt
     5161 actgcaaacc gtggcaaacc gctggcagcg tacagatgta tggcctgccc tggagcaatt
     5221 acttaaactg ggtccaatag aaatttaccc ggcacgtatt ccaaaagctc ctgatttctg
     5281 gcatccggca atgtggtcca ggccgcgcct tatcaagaat aatcagcctg ttaccgatga
     5341 cgctctggaa attatcggcg aaatgctgcg ctttacccag ggcggacgtt tttatggcgg
     5401 actggaacag ctgaaaacgt tttgccagcc gcaaacgctg gcagcttttg cctgggatct
     5461 tttcaccgcg tggcaacaag ctggagcccc cgcaaaagac aactgggcat ttctggcgtt
     5521 aagtctcttt ggtgacgaaa gcacggctcg ggatctgacg acacagatcc tcgcctggcc
     5581 acaagaaggc aaatctgccc gtgctgtcag tggcctgaac atccttaccc tgatgaataa
     5641 tgatatggcg ctgatacagc tgcaccatat atcccaacgg gcgaaatccc gctccttacg
     5701 tgataacgcg gcggaatttc ttcaggtggt cgcagaaaat cgcgggctaa gccaggaaga
     5761 gttagcggac agattagtcc caacactggg tcttgatgat ccgcaggcgt tgatttttga
     5821 ttttggtcca cggcaattta ccgttcgctt cgatgaaaac ctcaacccgg ttatctacga
     5881 tcaacaaaac gttcgccaga aaagcgttcc ccggttgcgc gccgatgacg atcaactgaa
     5941 agcccccgag gcactggccc gactaaaagg gctaaaaaaa gatgcctctc aggtgagcaa
     6001 aaacctgctc ccgcgtcttg aaactgccct gcgcaccacc cgacgctggt cgctggcaga
     6061 ttttcattct ctgtttgtta atcatccctt tacccgcctg gttacccagc gattaatatg
     6121 gggggtttat ccggcaaatg aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga
     6181 gggggagttc tgcaatgcac aagatgagtc cattgacctg cctgcagatg ctctgattgg
     6241 cattgcccac ccgttagaaa tggcagcaga aatgcgcagt gaatttgcac agctttttgc
     6301 cgattacgaa attatgccgc cttttcgcca gttggcgcgg cgcacggtgc tgctcacacc
     6361 tgacgagtca gccagtaaca gcctgactcg ctgggaaggt aaatccgcta gcgttggtca
     6421 acttatggga atgcgataca aaggctggga gtcaggctat gaggacgcat ttgtctatga
     6481 cttaggtgcg taccggctgg tccttaagtt ttcacccggc tttaaccact acaatgttga
     6541 tagcaaagcg ctaatgagct tccgttctct tcgggtttac cgtgacaata aatccgtcac
     6601 ttttgccgaa cttgatgtgt ttgatttgag tgaagcgtta agcgcacccg acgttatttt
     6661 ccattaaaac acagcgggca gtgatgcaac tgcccgttat caacacgacc taccaccggg
     6721 aaaagcgatg atcgtgcaaa aagagctggt tgctatttac gattatgagg ttcctgctcc
     6781 tgaagatccg ttttccttca gacttgagat ccataaattc tctgaattat ttacaggttc
     6841 cgtctatcga ctggaacgat tccggctaca tccaacattt catcaacgtg atcgagaaga
     6901 tgccgaccac taataaatga tgcgttgatt tatacaagag atgagtttat tgatgagcgg
     6961 aaattacgag gtgaatcaca tgaaactgta atagcaattt ttaattgtga actacagaat
     7021 atattcaacc aagaaataga ataatatact catcatactt caagtcgcag catatgcaac
     7081 ttgaagtatg gcgagtatag ctttattaaa gggggaaacg gtattccccc ttagatgcgt
     7141 ttcaatttgc ccgttacttc aacaaatcaa tcaaccgtaa cagagccact aatagctcca
     7201 gtacagcaat gagcacggtt aacttttgtg tcagcgtgtc catccgctct ccttattaag
     7261 gagcgcaaca ccagcacctt acccttacac tgcctgtcga tactggttta cgtatgttgg
     7321 accacgatgt tgcactccgg atcagggcac cacctgcagc accacccgtc cggtagccag
     7381 gacttgaaat aggttcgatc cggcgcaacg tcccaacggt ctggattata aatctcatta
     7441 tcttaattgc aacggggtcc agccgtggtt ataattcgtt cgttggaaca cgatgttgca
     7501 caggctgtgg tgttaggcct gaaaatagta aataaaagaa cctgcctcac cagcaggttt
     7561 ttttatttac tgtgatctgc tttccagata tttttcgctc aaacaactaa tgcgccaaac
     7621 atttattgcg cgtaaaatat cgtttatttc attaatacat ttcagggatg aatatatgtc
     7681 accgcagaat aatcatctgc agcgtccgcc tgccgctgtg ttatacgccg atgaactggc
     7741 aaaattaaaa aaaaatgatc acgcaccttg cccgcccggt tggcagttaa gtttgcctgc
     7801 ggcccgtgct tttatccttg gcgacgaagc gcaaaatatt agccgtaaag tggtgattag
     7861 tccctccgct gtcgaacgta tgttagtcac tcttgccacc gggcgcggtt tgatgttggt
     7921 gggggaacct ggcaccgcaa aatctctcct gtctgaatta ctggcaaccg ctattagcgg
     7981 cgatgccggg ttaaccatcc agggcggcgc atctactact gaagatcaaa tcaagtacgg
     8041 ctggaactac gccctgctta tcaaccatgg tccgtcaacg gaagcactgg tccctgcccc
     8101 gctttaccag ggaatgcgcg acggcaaaat cgtccgtttt gaagagataa cgcgtacacc
     8161 gctggaagta caagactgtc tgttaggtat gctttctgac agggtgatga cggtgccgga
     8221 actcaccggt gaagccagtc agctctatgc gcgggaaggc tttaatatta ttgccactgc
     8281 caatacccgc gatcgcggcg tcaatgaaat gagtgccgcg ctaaagcgtc gcttcgattt
     8341 tgaaactgtc tttccgatta tggatttcgc ccaggagctg gagcttgtcg ccagtgcttc
     8401 ggcgcgtttg ctggcccata gcggcattcc gcataaagtg cctgacgcgg tactggagtt
     8461 actggtccgc acctttcgcg atctgcgtgc caacggcgaa aagaaaacgt caatggatac
     8521 actgacagcg attatgtcca ccgctgaagc cgtgaacgtg gcccacgcgg tgggcgtccg
     8581 cgcctggttt ttagcgaatc gcgcaggtga gccggccgat ctggtggact gtattgccgg
     8641 aaccatcgtc aaagataacg aagaagatcg cgcgcgcctg cgccgttact tcgaacaacg
     8701 tgttgctaca cataaagaag ctcactggca ggcttattat caagctcgcc accgcctgcc
     8761 gtgaggaaag atgtatgagc gagccgttaa ttgtcggcat ccggcatcat agtccggcct
     8821 gcgcccggct ggcgaaatcg ttaatcgaaa gccagcggcc acgatacgta ttgattgaag
     8881 gcccggctga ttttaatgac cggatagacg aactgttttt agcccaccag cttccggtag
     8941 ctatttacag ttattgccag tatcaggacg gtgcggcccc cgggcgtggt gcctggacgc
     9001 catttgctga attttcgccg gagtggcagg cgctacaagc cgcacgtcgt attcaggcac
     9061 aaacttactt catcgatttg ccttgctggg cgcagagtga agaagaggac gattcgcctg
     9121 atacacaaga tgaaagccag accttactgc tgcgtgacac ccgcatggat aacagcgata
     9181 ccctgtggga tcacttattc gaagatgaaa gccagcaaac tgcattaccc tctgcactgg
     9241 cgcgctattt tgcccaactg cggggcgatt cccccggaga tgctctcaat cgtcagcgcg
     9301 aagcctttat ggcccgctgg attggatggg cgatgctgca aaacaatggc gacgtgttag
     9361 tcgtctgcgg cggctggcat gctccggcac tggcaaagat gtggcgcgaa tacacgcagg
     9421 aaattaacac gccggaattg ccctcgctgg cagatgccgt tacaggttgt tatctcaccc
     9481 cctacagtga aaagcgcctt gatgtgctgg caggatacct ttccggtatg cctgccccgg
     9541 tctggcaaaa ctggggctgg cagtggggcc tacagcaggc cggtgaacaa ctgctaaaaa
     9601 ctatccttac ccgtttgcgc cagcacaaat tgcccgcttc taccgctgat atggctgccg
     9661 cccatctgca tgcgatggcg ctggcacagt tgcgcggtca tacactaccg ttacgcactg
     9721 actggctgga tgccatagca ggatcgctga ttaaagaagc cctgaatgcg ccgttgccgt
     9781 ggagctatcg cggcgctatt catcccgata ccgatccgat tctgctaacg ttgatagaca
     9841 cattagcggg tgacggattc ggtaaacttg ccccttctac gccacaaccg cctctgccaa
     9901 aagatgtcac ctgcgaactg gaacgtaccg caatctctct tccggcggag cttaccttaa
     9961 atcgctttaa ccccaatgga ctagcgcaaa gtcaggtgtt acatcggctg gcaatactgg
    10021 agatcccagg gattgtacgc cagcagggaa gtacactgtc acttgcaggc aacggtgaag
    10081 aacgctggaa attaacccgc ccgcttagcc agcatgcggc attgattgag gccgcctgct
    10141 ttggtgcaac actccaggaa gccgcacgcc ataaattaga agccgatatg ctggacgcgg
    10201 gtggaatcgg cagtatcacc acatgtctta gccaggcggc gttagcgggt ctggcgtcct
    10261 tcagtcagca attactggaa caactcacat tattaatcgc ccaggaaaat cagtttgccg
    10321 aaatgggcca ggcgctggaa gtgctttacg ccttgtggca gctcgatgaa atcagcggta
    10381 tgcaaggcgc gcagatatta caaacgacgt tatgcgcggc tatcgatcgc acgctgtggc
    10441 tgtgtgaatc taacggcaga ccggatgaaa aggagtttca cgctcacctg catagctggc
    10501 aagcgctttg ccatatcctg cgcgatctac atagcggcgt taatttaccc ggcgtttctc
    10561 tttctgcggc ggtagcctta ctggagcgac gcagtcaggc gattcatgcc ccggcgctgg
    10621 atcgcggcgc ggctcttggc gcactaatgc gtctggaaca tcccaacgcc agtgccgaag
    10681 cggcgctgac gatgctggcg cagttatccc cgacacaatc cggcgaggcg ctgcacggtt
    10741 tgctggcgct ggcccgccat caactggcct gtcagccggc atttatcgct ggtttcagca
    10801 gtcatttaaa tcaactgagt gatgccgatt ttatcaatgc cctgcccgat ttacgcgcgg
    10861 cgatggcctg gctaccacca cgagaacgcg ggacgctggc gcatcaggtg cttgagcatt
    10921 atcaactggt gcaacttccc gtttcggcgc tgcgaatgcc gttgcattgt ccaccacaag
    10981 acattgcaca tcatcaacaa ctcgaacagc aggcactggc atcgctgcaa cactggggag
    11041 ttttccatgt ctgaactgaa cgatcttctg accacccgtg agctacaacg ctggcgatta
    11101 attcttggcg aagcggcaga aacgacgctt tgtgggctgg atgacaacgc ccggcagata
    11161 gaccacgcgc tggagtggct gtatgggcgc gatcctgaac ggctccagcg tggtgaacgt
    11221 tacggtggat taggtcgttc aaatctcacc acccctgagt ggatcaacag cattcacacg
    11281 ctgtttccgc aacaggtgat tgagcggctg gaaagcgatg ccgtgctgcg ctacggcatt
    11341 gaagatgtgg tgacgaatct cgacgtgctg gaacgtatgc agccttctga aagcctgcta
    11401 cgcgccgttt tgcacaccaa acatctgatg aatcccgaag tactggctgc cgcccgccgg
    11461 atagtgcacc aggttgttga agaaattatg gctcgactgg caaaggaagt tcgtcaggct
    11521 ttttctggag tacgcgatcg ccgccgtcgc tcatttattc cactggcgcg taatttcgat
    11581 ttcaaaagta cgctgcgtgc caacctgcaa cactggcacc cgcaacacgg caagttgtat
    11641 atcgaatccc cccgctttaa cagccgcatt aaacgccata gcgaacaatg gcaactggtc
    11701 ctactggttg atcaaagcgg atcgatggtt gattcggtga tccactccgc agtgatggcg
    11761 gcctgtttat ggcagttacc cggcattcgt acccatctgg tcgcttttga caccagcgtg
    11821 gttgatctca cggcagacgt tgccgatccg gtagagttat taatgaaagt acaactgggc
    11881 ggcgggacca atatcgccag tgccgtggag tatggtcggc aacttattga acaacctgca
    11941 aaaagtgtca ttattctcgt gagcgatttt tatgaaggtg ggtcatcatc attgctgacg
    12001 catcaggtga aaaagtgtgt ccagagcggc atcaaagtgc tggggctggc agcgctcgac
    12061 agcaccgcaa cgccttgcta tgaccgcgat atggcccagg cgctggttaa tgtcggcgca
    12121 caaatagccg caatgacacc gggcgagctg gcttcctggc ttgcggagaa tcttcagtca
    12181 tgaattcact acgtccggaa ttattagaac tgacaccaca ggcactgacg gcgttaagca
    12241 atgccggttt tgttaagcgc agccttaagg aactggaaaa tggcaacgtc ccggaaatca
    12301 gtcatgagaa cggcgctata atcgccacgt tcagtgacgg tgtccgtacc cagctggcga
    12361 acggccaggc actgaaagag gctcagtgca cctgcggggc cagcggcatg tgccgtcatc
    12421 gcgtgatgct ggtgttaagt tatcaacgac tttgtgccac cgcccagcct acggaaaaaa
    12481 aagaagaaga gtgggattcg gcaatctggc tgaaagaact ggctaccttg cccgatgcca
    12541 cccgcaaacg cgcgcaggcg ctggtcgcta aaggcatcac cattgagttg ttctgtgcgc
    12601 ctggcgaaat tccctctgcc cgattaccga tgagcgatgt gcgtttttat tcccgcagca
    12661 gcattcgctt cgcacgctgt gattgcattg aaggcacact ttgcgaacat gtcgtactgg
    12721 ctgtgcaggc cttcgtcgag gccaaaactc agcaagcgga atttactcat ttaatctggc
    12781 agatgcgcag tgaacacgtc acatcatctg atgatccgtt tgccagcgaa gaaggcaaaa
    12841 cgtgtcgtca atatgttcag caattaagcc aggcattatg gctgggcggg ataagtcaac
    12901 cgccaatcca ttacgaggcc gctttcagtc gcgcgcagca agcggcggaa cgctgcaact
    12961 ggcgatgggt gagtgaatcg ctacggcaat tgcgcgccag tgttgatgcc ttccacgccc
    13021 gtgccagcca ctaccatgcc ggagaatgct tacgtcagct tgcggcatta aacagtcgat
    13081 taaattgcgt acaagagatg gcgcgacgcg acagtatcgg tgaagttcct cccatgccgt
    13141 ggcgcacggt cgttggcgct ggtattgccg gagaagcaaa acttgatcat ctgcggctgg
    13201 tgtctttagg tatgcgttgc tggcaggata ttgagcagta tggtttacgc atctggttta
    13261 ccgatcccga cacaggaagt attttgcatc tttcgcgtag ttggcagaga agcgaacagg
    13321 aaaactcacc ggcagcgacg cgtcggctgt ttagttttca ggctggcgca ctggccggtg
    13381 ggcaaattat ttcacaagca gcaaaacgca gtgccgatgg cgagctgctg ttagctaccc
    13441 gcaaccgctt aagcagcgtt gtgccgctgt cgcctgatgc ctggcaaatg ttgagcgcac
    13501 cgttgcgcca gccgggcatt gtggctttgc gggaatattt acgccagcgt ccccccgcct
    13561 gcatacgacc aatcaaccag gtcgataact tatttattct gccgatagcg gagtgtattt
    13621 cgctcggttg ggatagcagc cgccagacgc tggatgcgca ggtaatcagc ggcgaagggg
    13681 aagataatgt gctgacgtta tcccttccgg catctgcctg ttctcctttt gccgttgaac
    13741 gcatggcggc gcttttgcaa caaacagacg accccgtgag tctggtttct ggctttgtca
    13801 gttttgtaga ggggcaattg acactggaac cacgggtgat gatgacaaaa acccgcgcct
    13861 gggcgctgga cgcagaaact gcgcctgtgg caccgctacc ttctgccagc gttctgcctg
    13921 tgccgcctac cgctcatcag ttgctgatgc gctgccaggc gttacttatt caactgctcc
    13981 ataacggctg gcgctatcag gaacagagtg ctattggtga ggcagagttg ctggcgaatg
    14041 acctcaccgc ggtgggtttt tatcggctgg cacatgtgtt gggacaattt cgtaatacag
    14101 aaagcgaggc acgggtagaa gcaatgaata acggtgtttt gctttgcgaa caattattcc
    14161 ccatgcttca gcaacaagga tgaaatagtg ctttttatta agagttctac tcctgttccg
    14221 gactgctcac gccacagtat taggcatatc ctatatagcc cctggtgaga gtcaccagtt
    14281 ccttgattaa ataaaatgga gttttacatg aaggctttca ataagctgtt ttcccttgtt
    14341 gttgcatctg ttctggtttt ctctcttgct ggctgcggtg acaaagaaga atcaaagaaa
    14401 ttcagcgcca atctgaacgg cactgaaatt gccattacct atgtctacaa aggtgacaag
    14461 gtgcttaagc aatcttctga aaccaaaatt caatttgcct ccattggtgc aaccaccaaa
    14521 gaagatgccg ccaggacact tgagccgtta agcgctaaat acaaaaacat cgcgggtgtt
    14581 gaagaaaaat taacctatac cgatacctac gcgcaggaaa acgtgactat tgatatggaa
    14641 aaagtggatt ttaaagccct gcagggtatt tcaggaatca acgtttctgc tgaagatgct
    14701 aaaaaaggta tcactatggc gcaaatggaa ctggtgatga aagccgctgg ttttaaagaa
    14761 gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc atacccgtct tagcttttct
    14821 tcacatgctg gcgcgctgcc agtccacgca gaaaataacg taaaaattga tcgccgcatt
    14881 cgcggaagtt tttatgatcc ggtgcacgca tcatcgccgt aatttccggc atcgaaacgc
    14941 ggaactgctg ttcggtgagg attgccagaa tgtcatcggt tttaagcgaa aacgcgatac
    15001 gtaatttttt cagcacgatg ttgttattaa tgcgacgttc cggctccagt gccggagcag
    15061 actcatcctt gccgcgtttt tcataaatca ggccattgag gaatgaagac aaaacaatgt
    15121 ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg atctgttccg
    15181 cggtggcttc gacattaccc agcgccagaa tacgcaccag gtcattatta ttggctttca
    15241 aaatgtagcg cacgctgcgc agaatatcgt tacttagcat gaggccttca ggtgttgatg
    15301 aggcaaaaag ccattttagc agtcttttac aggccaatcg cctcttttaa gcttttcaga
    15361 taacggcggc tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc ctggccgtta
    15421 tcttccagac ggatctcctg taaatgcgcg aggttaacca gatactgacg atggcagcgc
    15481 agtagtggtg tacgactttc cagggtacgt aatgtcagtt cggtaaagcc ctctttccct
    15541 tcgtgactgg taacgtagac accgctcatc cgactgctga caaatgccac atctttcatt
    15601 tgcagcaaat aaatccgact atgccccgta caagggataa atttcagcgc ctgttgattt
    15661 tccggtaaca acgaaacatc ctgcttgctg cgctcctgac gcaatcgcgc cagcgttttc
    15721 tccagtcgcg cttcatcaat tggcttcagc agataatcaa aggcatgttc ttcaaaggct
    15781 ttaatggcgt attcgtcaaa cgcggtgaga aaaacaatat acgggcgatg ttccgggtcg
    15841 agcatcccca ccatttccag accactgatg cgcggcatct ggatatcgag aaacagcaca
    15901 tccgggcgca gtttatgcac cgcgccgatc ccttctacgg cgtttgaaca ctctccaacg
    15961 atttcaatat cgctctgctc ctgcaaaaat acgcgcaggt tctcccgtgc aagcggttca
    16021 tcatcgacaa ttaagacttt aatcatgcct cgtccctcca tggtagtcgt aacgttattc
    16081 gggtgtaact atcaggctca caggcgacgc ttatcccata gtcatcgcca aaccgttcac
    16141 gtaaacgctt atccaccaga ttcatcccca gcccactggc attggttacc ggttgataca
    16201 aaccggcatt gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg gcgctgattg
    16261 ccacttgccc tgtatccagc agttgtgatg tcccatgttt aatggcgttt tccactatcg
    16321 gttgcagggt aaacgcgggc aattgctgct gggataattc ttgcggaata gcaatgttga
    16381 cctgcaaccg cgactggaag cgcgcctttt caatttgcag ataagcgttc acatgttcaa
    16441 tttcgtcggc gagagtaaca aactccgaag gccgctttaa gtttttgcgg aaaaaagtgg
    16501 aaagatactg caccagttgg ctggcctgtt cgctgtcgcg gcggatcacc gctttaatgg
    16561 tgttaagcgc attaaacaaa aaatggggat tcacctgggc gtgaagcagt ttgatctctg
    16621 actgggtgag catcgctttt tgccgctcat attgcccggc gaggatctgc gccgaaagca
    16681 gttgcgcaat cccctcgccc agcgtgcggt tgattgaact gaataaacgg tttttggcct
    16741 catacaattt gatggtgccc atcacccgct gattttcgcc acgcaacgga atgaccagcg
    16801 tcgaccccag tttgcattgc ggatgcaaag agcaacgata aggtacttcg ttgccatcag
    16861 cgtagaccac ttcaccggtt tcaatcgctt ttaaggtata agtcgaagaa atcggtttgc
    16921 cgggtaaatg gtggtcgtca ccaattccgg taaaggccag caatttctct cgatcggtaa
    16981 tcgcgactgc accaatatcc agctcctgat acagcacctg tgccactttc atgctgttca
    17041 cttcgttaaa cccctggcgc aaaatgcctt ccgtcgaggc cgccactttc agcgcagtgg
    17101 cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat atacgcataa
    17161 acaacgccgc gccgacggta ttggtgacca tcattggcgc agcaatatta ctcaccagac
    17221 gcaccgcatc ttcataaggt cgggcgatcg ccaggataat caacatttgc accatttcag
    17281 cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac tttatcagtg cgcccgcgac
    17341 ggatcaggat gctgtgtacc aggccaccga gcaatccttc aacgatggtc gagatcatgc
    17401 aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa cccgccggtc agaccaacca
    17461 gcccaccgac gaccggaccg ccgagtaagc cgcccattac cgcacctatc gcacgggtat
    17521 tggcaataga atcgtcaatg tgcaacccaa accaggtgcc catgatgcag aagatggaaa
    17581 agacgatgta gcagagaaat ttatgcggca gacgaaccgt gacctgcatt aacggtatga
    17641 ataatggcgt tttactcatt aaccacgcaa tgactaaaaa aacgcacatc tgctgaagca
    17701 gcagcaacac cagattaaaa tcgtacatac ccgcaaacca cacttccctt taaaacgcgt
    17761 aacatacatt gcttgcgttt aactttcttt gaactcttgc agaaaaatga gaattcgtga
    17821 gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc ctatagatgc acaaaaaacg
    17881 ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa accgggacct cgcgagcaag
    17941 ggtgagacga tggcgcttta cacaattggt gaagtggcgt tgctttgtga tattaaccct
    18001 gtcacgttac gcgcgtggca gaggcgttac ggattgctga aaccgcaacg gacagacggc
    18061 ggtcatcggc tgttcaacga tgccgatatt gaccggatcc gcgagatcaa acgctggatc
    18121 gacaacggcg tgcaggtcag caaagtaaaa atgctgctca gtaatgaaaa tgttgatgtg
    18181 cagaacggct ggcgcgatca gcaagaaaca ttactgactt acctgcaaag cggcaatctg
    18241 catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca gacactcacc
    18301 acacatctgt ttattcctct gcgccgacgg cttcagtgcc aacaaccgac tctccaggcg
    18361 ctgctggcga tcctcgacgg cgtactgatc aactacatct ctatttgcct ggcttcggcg
    18421 cgcaaaaaac agggtaaaga tgcactggtg gttggctgga atattcagga taccactcgt
    18481 ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt cctcgcccac
    18541 tcgctcaatc aactacgccc tgaactgttc gaaggccgta cattgctggt atggtgcggt
    18601 gataatcgaa cctccgccca acagcagcaa ctcaccagtt ggcaggaaca aggccatgat
    18661 attttcccac tcggcattta atgattcgtt aacaaatacg ctttactgta caatcctttc
    18721 gttaacataa ggagtgcatt atgcgcatag ctaaaattgg ggtcatcgcc ctgttcctgt
    18781 ttatggcgtt aggcggaatt ggtggcgtca tgctcgcagg ttataccttt attttgcgtg
    18841 ctggctgaac gcctgcacca gcctttcaaa caggcggtct gcgatgatcg ccgccagtgc
    18901 caccagtaac gctccctgga tcacatacgc ggtattaaat ccgctaagtc cgatgatgat
    18961 gggcgtaccc agcgtgttgg cccctaccgt tgaggcgatc gtcgccgtgc caatgttgat
    19021 aatcaccgaa gttcgtacac ccgccagaat caccggagct gccagcggta gctcgacctt
    19081 acgcagtcgc tgaccacgac tcattcccat acctttcgca acttctgtca cgctggcatc
    19141 aatcgctccc agcccggcaa gtgtcgcctg caggacgggc agcacgccgt aaaggatcaa
    19201 ggcgataatc gctggtttca gaccaaagcc gatcaccgga acggcaatcg ccagcaccgc
    19261 gacgggcgga aaagtctgcc caacggcggc aatagtttcc accagtgggc gaaattccgc
    19321 accccacggg cgagtgacag caattccggc accagtacca atgatcaccg caaacaaact
    19381 cgaaattccc accagccaga aatgagccag tgccagagct gcaaagcttt cctgctgata
    19441 aacgggtcgt ggcagttgtg ggaacaaggc agcaaacagc ggctggctgt aaggcagcca
    19501 gaaaatcagc gccacaaaca gagcaatcag ccagaacagc tgatcgcgca acatcttcat
    19561 acgattacgc ctcctccagc agatcctgaa aatgcagcgt gccgcaaggc tcgccctgcg
    19621 tgttcaccac cggcagcact tcacatcccc gcgcgacaaa cagggagagc gcatcgcgta
    19681 gcgtcatctc ttctgccagc gcctcacctt ctgcccattc ttcgcgacgc acgtaatccg
    19741 ccacgctacg taacgaaagc aggcggacac ccagttcact atgcccaaaa aactggcgga
    19801 caaaatcatt cgccggatga gtcagcatcg ccagaggatt gccctgctgc acgacttcac
    19861 cgtgatccat caataccaga tgttctgcca gccgtagcgc ctcatcaata tcatgagtga
    19921 ccagcacaat agtgcgcccc tgcaaacggt gaatgcgcgt catctcttgt tgcaacgcgc
    19981 cacgcgttac cgggtccagc gcgccgaatg gttcatccat cagcaagact tgcggatcgg
    20041 cagccagcgc gcgcgccact cccacacgtt gctgctgacc accggaaagc tgatgcggat
    20101 aacgctcacg caaatttggc tccagcccca gtagcgccat taattcgtcg atacgatcgt
    20161 cgatccgcgc ccgtgaccat ttttgtaatt gcggcacggt ggcaatattt tgcgccacgc
    20221 tccagtgggg aaacaggcca atagattgaa tggcatagcc catccggcgg cgcaactcca
    20281 gcactggcag cgagcgaatt tcttctccgg caaagcggat cacgccactg tcatgctcca
    20341 ccaggcggtt aatcattttc agagtggtgg atttaccgga gcctgatgta ccaatcagca
    20401 ccgaaaaact cccctcctga aaattgagat tgagatcgtt aacggctttt tgtgtgccga
    20461 acagtttgct gacatggcta aattcaatca ttacgtttca ccttcagcag tgcgataagc
    20521 aaatcgaaca gcgcgtcggt aagcaccgcc agaacaatta ccgggatcac ccccagcaac
    20581 actaaatcaa tggcactgct taacagcccc tggaaaacca gcgcaccaaa accgcctgcg
    20641 ccgattaacg ctgcaatcac cgccatgcca acggtttgca ccatcaccac ccgcaggctg
    20701 cgcagaaata ccggtaacgc cagcggcaac tgaacatgca ggaaacgctg cgccccgctc
    20761 atccccatcg ctctggcgct ctccagcaca tcgcgcggga tctggttcaa gccgactacc
    20821 acgccgcgca ccagcggcag caaggcatag agcaccagcg caatcagtgc gggtgtcatt
    20881 ccggttcctg ctatgccgag cgttcccagc cacggaaagg ccgtaaccag cgcggcaagc
    20941 ggcgcaatca gcaggccaaa gagcgctacc gaaggcacgg tctgaatgac attcagcaga
    21001 gaaaaaatcg ccccctgccg cgcggtggaa aagtagcacc agatgcccaa cggcacacca
    21061 atcactaacg caggcagcac cgcaccaaac agcaacgtca gatgttgcgc cagcgcgtcg
    21121 tcaaacacat cctgacggtt ggcgtattct ttcattagtg agagatcgtt aagcgtgccg
    21181 gagtacagca accacagcgg aatgatggca atctgcatat gcaacaacca gcgccacagc
    21241 ggatgcgtgg agattcggcg gatggcatcg ctacaggcca gcagtgccag cgccgcagcc
    21301 agccagaaac cacttccgag gctggtacgc gccagcgcac tgccattttg cgccagttgt
    21361 gtcgccgcct ttccagctcc ccacaccagc aatacgaaga cgaattgcgc cagaatgagt
    21421 gcaaaaatgc tgcctttttt agcgggaata aaacaggctg tcagccaggc gcaaccaacg
    21481 cccaccagca tccagagagt ttgcggccac agctgccaga gatgacgccc ctcacccgaa
    21541 actaaacgat taggtgcgta actgataaac ggcagcgccg ctgcgattgc cgtcagcaac
    21601 agcagcagcg ccagaacagg attaatacgg aaataagtca cgagaaatta cttcgtccac
    21661 cctttttgtt tcaggtagtc ggctgccact tttttggcat ccagtccttc cactgcaatg
    21721 ctggcattca gctgctgcaa tgttttttca tcgaggctgg cgaagactgg ccgtagccac
    21781 tgtgccattt gcggatactc cttcaacacc gattcacgca ccactggtgc aggcgcgtag
    21841 ataggttgca caccttgcgg atcgcttaag gtttgcagcc ccagcgccgc aaccgggccg
    21901 tcggtgccgt aagccattgc ggcattaaca ccagaagttt gctgggcagc ggctttgatc
    21961 gtcaccgccg tgtcaccacc ggccagtgac agcaactgat cctgacctag cttaaagcca
    22021 taggcttttt caaacgcggg taaggcatcg gcgcgttcga taaactctgc cgaggctgcc
    22081 agtttgaagt tgccaccctc tttcagataa cgactcagct cggcaagcga agtgagtttg
    22141 tttttctctg ccacatcctg acgcacggcg atggtccagg tgttatttgc aggcgcgggc
    22201 gtcagccaga ttaacttgtt ttgctctgca tcgagttttt tgactttctc atagccttgc
    22261 tgcgcgtttt tccatgccgt atcgttttca tctttaaaga aaaacgcgcc attgccagta
    22321 tattccggat agatatccag ctcgccagaa gtgatcgccc cgcgcactac gggagtcgtt
    22381 cccagttgca ctttatttac cgtaggaacg ccgtgacttt ccagcacctg caaaatgata
    22441 ttgccgagca gcgcgccttc cgtatcgatt tttgaaccaa ccttaacggg ggaagccgcc
    22501 tgtagcggca ggctcacggc tgccaacaaa accagtgaac ctgcccagac ctttgagagt
    22561 ggcatgatgc tttcctcatt cttttactgt tgttttcagc gaattaagag aaaagcatag
    22621 ttgataatga cagggttagc ctgtctggac ggattcagtt gcagaatcag ataaatactt
    22681 aaagggcata ttttgcccgg cgtttcttgc cggatgcggc gtaaacgcct tatccggcct
    22741 acaaacatcg aaaattacaa caactcaaac tcggctttct taacgcgtgc ggaatcagtg
    22801 ccgatgaaga cattgaactt gccaggctcg gcatcatatt tcatctgttg attccagaac
    22861 ttcagcgcct caatatcgat tgggaagctg acggtctgag tttcgcccgg tttcagggtg
    22921 attttctcaa agcctttaag ctgcttcacc gggcgactca tggaagccgt cacatcctgc
    22981 aagtacatct gtactaccgt ggccccttcg cgtttaccgg tgttcgtcac ctgcacgctg
    23041 gccgtcactt tgccgtcacg cttcatggtc ggcgcggaaa gtttcacatc agagacggtg
    23101 aaggtggtat agctcagacc atagccgaac ggataaagcg ccccgttagc ttcatcaaaa
    23161 taacgcgatg tatatttgtt cggcttgtcg gcattatacg gacgcccggt attcagatgg
    23221 ctgtagtaca ccgggatctg cccgacagaa cgcgggaagg acatcggcag cttgccggac
    23281 gggttgtaat cgccaaacaa tacatcggca attgcattcc ccccttccgt acccgcaaac
    23341 caggtttcca gaatcgcatc agcctgctga tcttctttca ccagcgccag cggacgccca
    23401 ttcatcagca ccagcaccag cggtttaccg gtggctttca gcgccgcaat caagtcacgt
    23461 tggctttgcg gaatagtgat atcggtacgg ctggaagcct cgtgcgccat cccctgagct
    23521 tcaccgacca cagccaccac cacatcagat tgtttcgcgg tctgcaccgc ttcatcaatc
    23581 atctcttgcg gcgaacgcgg atcgactttg accgcttctt catactgatt caggaaatcg
    23641 ataatgcctt tgtcactggt aacgttcgcc cctttggcat acagcacttt accgttttca
    23701 ccgacggcat ttttaatccc tgttagcaca gtaacagatt gatcggcgac acctgctgcc
    23761 gaccagcttc ccatcacgtc acgcttgctg tctgccagcg ggccaaccac cgcaatggtt
    23821 gcagattttt tcagcggcag cgtttcgaga cggtttttca gcaacaccag gctttcgcgc
    23881 gccacttcac gcgcttcttt acggtgcagg cggctttcgg cattggtatc caccgggtca
    23941 gactctttcg gccccaaatg gctgtatggg tcgttaaaca atcccatatc atatttaacg
    24001 ttcagtacat ggcgggcagc atcgtccagc tcttccatcg tcactttgcc ggatttgatc
    24061 aacccaggca gatacttcga gtaatactcg tcgctcatgc tcatgttgat tccggacttc
    24121 agcgccacgc gtaccgcatc ttccgggtct gccgccgtgc catgtttaat cagttctttg
    24181 attgcgccgt gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg cagaacatct
    24241 ttcagcagcc aggagtcgga ggtggctggc gtgccgttca gcgagttcag cgccaccatc
    24301 accgcaccgc tgcctgcatc cagccccgct ttgtacggcg gcatatagtc gttaaacagg
    24361 cgctgcggac tcatatcgac ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg
    24421 gcaaagtgtt tgacgctggt catcaccgag taacgatctg ccgggctttt accctgcatc
    24481 gcttccacca tggttttgcc cattattgag gtgagatacg tatcttcgcc aaaaccttcg
    24541 gaagcacgtc cccagcgcgg atcgcgcgag acatcgacca tcggtgccca ggtcatattc
    24601 aggccatcat ctgccgcttc ataagcagag acacgcccga ctgttttcac cgcatcgagg
    24661 ttaaaagacg aggccagacc gagactaatc gggaacaccg tgcgctgacc gtgcagcacg
    24721 tcgtaagcaa agaaaagagg aattttcagg cggcttaatt ccatcacctg atcctgcatg
    24781 gcgcggatat cctgacgggt tacagtgttg aaaatcgccc caacctgacc gtctttgatc
    24841 atctcgcgga tcgcctcttt cgggttatcc gggccgacgc tgattaagcg cagctgacca
    24901 attttctcgt caactgtcat tttcttaagc agttcggtga cgaacgcatc ccgcgcttcg
    24961 ggcgttaatg gatggttgcc gaataaatca tccgccagtg ctggctgcag ggccagactc
    25021 accgcgattc ctactgaaca tagccatttc atatggattt ttcttccttt gttgcccgac
    25081 gtggcagcga aaatggtgca aaaaccgtag tttgccataa gcatgatgga gagagaaaaa
    25141 gaatgctcag tttattgtct gaattttcaa aatattcact cgctgaattg tcatacaagg
    25201 cgctattcta gtttgtgata ttttttcgcc accacaagga gtggaaaatg tcttccatga
    25261 caacaactga taataaagcc tttttgaatg aacttgcccg tctggtcggt cattcacacc
    25321 tgctcaccga tcccgcaaaa acggcccgct atcgcaaggg cttccgttct ggtcagggcg
    25381 acgcgctggc tgtcgttttc cctggctcac tactggaatt gtggcgggtg ctgaaagcct
    25441 gcgtcaccgc tgacaaaatt attctgatgc aggcggccaa tacaggcctg accgaaggat
    25501 cgacgccaaa cggtaacgat tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg
    25561 acaagctgca cgttctcggc aagggcgaac aagtgctggc ctatcctggc accacgctct
    25621 attcactgga aaaagccctc aaaccgctgg gacgcgaacc gcactcagtg attggatcat
    25681 cgtgtatagg cgcatcggtc atcggcggta tttgtaacaa ctccggcggc tcgctggtgc
    25741 aacgtggccc ggcgtatacc gaaatgtcgt tattcgcgcg tataaatgaa gatggcaaac
    25801 tgacgctggt gaaccatctg gggattgatc tgggcgaaac gccggagcag atccttagca
    25861 agctggatga cgatcgcatc aaagatgacg atgtgcgcca cgatggtcgt cacgcccacg
    25921 attatgacta tgtccaccgc gttcgtgata ttgaagccga cacgcccgca cgttataacg
    25981 ctgatccgga tcggttattt gaatcttctg gttgcgcagg gaagctggcc gtctttgcgg
    26041 tacgtcttga taccttcgaa gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc
    26101 agccagaagt gctgaccgaa atccgccgtc atattctggc taatttcgaa aatctgccgg
    26161 ttgccgggga atatatgcac cgggatatct acgatattgc ggaaaaatac ggcaaagaca
    26221 ccttcctgat gattgataag ttaggcaccg acaagatgcc gttcttcttt aatctcaagg
    26281 gacgcaccga tgcgatgctg gagaaagtga aattcttccg tccgcatttt accgaccgtg
    26341 caatgcaaaa attcggtcac ctgttcccca gccatttacc gccgcgcatg aaaaactggc
    26401 gcgataaata cgagcatcat ctgctgttaa aaatggcggg cgatggcgtc ggtgaagcca
    26461 aatcgtggct ggtggattat ttcaaacagg ccgagggcga tttctttgtc tgtacgccgg
    26521 aggaaggcag caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca attcgttatc
    26581 aggctgtgca ttccgatgaa gtcgaagaca ttctggcgct ggatatcgct ctgcggcgta
    26641 acgataccga atggtatgag catttaccgc cggagatcga cagccaactg gtgcacaagc
    26701 tctattatgg ccattttatg tgctatgtct tccatcagga ttacatcgtg aaaaaaggcg
    26761 tggatgtgca tgcgttgaaa gaacagatgc tggaactgct tcagcagcgc ggcgcgcaat
    26821 accctgccga acataacgtc ggtcatttgt ataaagcacc ggagacgttg cagaagtttt
    26881 atcgcgagaa cgatccgacc aacagtatga atccggggat cggtaaaacc agtaagcgaa
    26941 aaaactggca ggaagtggag taataataac ggatggcaga gtatcgccat ccgtcattca
    27001 tttaatcgtt ctgtgccgtc tgccccgccg ccgccatttg ggcggctttt tgttttttat
    27061 agctcaacgc tgctgccgga acaggcatca ctttaccggt ttcaatccag gtacgcaggc
    27121 ggctggcatc ggcaaaatgg gtatatttgc caaacgcgtc catcactacc agcgccaccg
    27181 gtttattatt gataaccgtg cgcatcacca gacaatggcc cgccgcattg gtaaaaccgg
    27241 ttttggttaa ctgaatattc cagttatcac gatacaccag atggttagta ttgcggaacg
    27301 gcagcgtata cgtcgggtta gagaaggttg ccatatcttc ccgggtagta cttaactgcc
    27361 cgatcaacgg atattgtttg ctggcaataa gtagtttggt taagtcacgg gcggttgaaa
    27421 cgttatgcac cgacaatccg gttggttcaa caaagcgcgt gttgttcatt ccgagcgatt
    27481 tcgctttcgc attcatcgcc ttaataaagg ctttgtaacc accgggataa tggtgcgcaa
    27541 ggcttgccgc cgcgcggttt tctgaagaca tcagcgccag caacagcata tctttacggc
    27601 tgatttcgct gttcagtcgt acgcgcgaat agaccccttt catctccggc gtctggctga
    27661 tatccacttt tagtttttca tccagcggca gtcgtgcatc cagcacaacc atcgcggtca
    27721 ttaatttgct gatagacgca atcggacgca ccagatccgg gtggttcgaa tagatcactt
    27781 tgttggtatt cagatcaaca atcatcgcgc taccggaggc aatttccggt tgtgacgcgg
    27841 tggtagcggc tgccgttttc gcaacggcct gcggtgcaaa aggcacagcc agcatcaggg
    27901 ccaggctaaa taaagaaact cgaaatttcg gcatgatgag cattcagata gtggttcacg
    27961 cgcacgggtt gcgcaccgcc ggagtaagga tttactgagg ctagcgacgc catcataacg
    28021 agcaaaaagt gcgatcgtca aagaagaatc gtgaggaaat gctgcattgc tgacatttac
    28081 gccagcaatg cagcgcaaaa gaactttcta gaacaaacga taaccgtagc cccatagtat
    28141 aacggtaagg gcgagcagca cttccagcac cagcacgcct atcgccagtg tcgaactgga
    28201 gaagctaagt ccttcctctt tgttgatatt caggaagctc ggaataccga gatacagcag
    28261 gtagccggtg taaaacagcg ccaccgtgcc gaccagcgca cacaaccaga ccagcgggta
    28321 aagcgccacc agaccgctta aaaacagcgg agttgcaacg tagcccgcga agaccatgca
    28381 gtgcgcaagt gacggacgct gcggataatt acgcgccatc caccagatga cccgccccat
    28441 caccgcaacc ccagccagca taacgccgta aaacaagaca gccagcgcca gtccggtaaa
    28501 ccaggataac ttcaggatag tgccatcgcc aaaattccag ccaatctgtg tagtgccaat
    28561 gaaggcgcaa atcaccggaa tcgccgccat cagcaaaacg tggtgggtgt agtgatgaga
    28621 aatcgtttcg ttttcgcgat taatcacctg catttcacga tcgggatggg aaaacagtcc
    28681 ccagacatgg ctcataccgc ccccttgttg tgagttcatg aacctgacag ttcaagtata
    28741 agtcagcttg tgattatttt ttgttcgctc atgtaatttg caccgtctga tgtacgtcct
    28801 ttttcagcgt gaatgattca cagggtgtat gcttacaggc aagcaaaggg agacagactg
    28861 tcctatggat ctcaatacac tcatctcaca atatggttat gccgcgctgg tgatcggtag
    28921 cctggcggaa ggtgaaaccg tgactttgct gggaggcgtt gcggcacatc aggggctatt
    28981 aaagttcccg ctggtggtac tttctgtagc gcttggcggc atgattggcg accaggtgct
    29041 ttatctgtgc gggcggcggt ttggcggcaa gctgttacgc cgtttctcga aacatcagga
    29101 taaaattgag cgggcgcaga aacttatcca acgccatccg tatctgtttg tcattggtac
    29161 gcgttttatg tatggctttc gggtcattgg cccgacgctg attggtgcca gccagctgcc
    29221 accgaaaatc tttctgccgc tgaatattct cggcgcattt gcctggtcgc tgatttttac
    29281 cactatcggt tacgctggtg ggcaggtgat tgcgccgtgg ttgcacaatc tcgaccagca
    29341 tttgaagcac tgggtctggt tgattctggt cgtggttctg gtggtaggcg tgcgctggtg
    29401 gctgaaacgg cgcgggaaga aaaagccgga tcatcaggcg taaaactatt gccctggata
    29461 aggtgttcgc gccgcatccg acatccggtg caccatgcct gatgcgacgc tggcgtgtct
    29521 tttctggcct aacccaatga aataaccacg cacctcactg cgctattctg ggttgaactg
    29581 cggattcgcc aacataaagc cgccatccac tatcaacgac tgcccggtgg tgtaatttgc
    29641 gccctccgaa caaagccata ccaccaggct ggcaatctca tgcgttgtgc caaaacgtcg
    29701 caagggaatc gaaggctccg cgtcaggctt cacgtcgccg ccatccatgc cgttcattgg
    29761 cgtggcgatc gccccaggtg cgactgcgtt caccaaaatt ttatgcctga ccagctcaag
    29821 cgccattgct ttggttaacc cgccgagcgc atgtttagcg gctgtgtagg cgctggcatc
    29881 cggcagcggc gtatgttcat gtaccgatgt aatgttgatg atgcgaccgc cctgcccttg
    29941 cttcaccatc tggcgggcag caatttgcga gcataaaaat gcaccatcga catcaacggt
    30001 aaaaatcttg cgccactcat cgaaagccat atcaagaaac ggcgctttgg tcattgcgcc
    30061 cgcattattc accagcacat caatacgccc cagccgttga ataagtttct ccagcgcctg
    30121 cgcaccttct ggtagattgc cgagatccag ttgcacgatc tccgcacgta cgccgtggct
    30181 aactacctca cgcgcggtat cttttgcccc ttcttcatct gagtgccagg taatgccaat
    30241 atcaaacccc tgctgcgcca gcaataacgc gcactctttg ccgatccccg aatcggaggc
    30301 ggtaattatc gcgacctgtg ccatcgagtt ctccacttaa cgctgaataa acgttaagta
    30361 tagaaggcgc atatcatcag cgtttgtacc ccccgcccaa cgcaccagtg agttgaatgg
    30421 aggcatccag ccactgccct tgcaataaca ggccattggc ccgctcacgc agcgcgggga
    30481 ttttggcttc gctgacgcgg gaaccggcaa tgatgcccgc gttaaagcgc gcctgcgcaa
    30541 gacctactac acgcaaggca tcgcgctcaa tttgcgcctg atgctggttt ttctccgcca
    30601 gtgtctgaac ctgactggct gcctgcgcca cgtcattcac cgcttcaacc accgctttgt
    30661 tgtagctggc gatagacaag ttgctttcgg cttttgcgat atcgaggttg gcgttaagac
    30721 gaccactatc gaaaatgggt agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat
    30781 gacggaacag atcgcttaag tgcaacgcat cctgttgcag gaaggccatc aggttgatgt
    30841 caggataaaa tgccgctttt gccgcatcaa tagtgcttaa cgatgactca acgtaccagt
    30901 gcgccgcctg caaatctgcc cgccgggcca gtaaggagta ccccagttca tcaggaagct
    30961 ggcttgccac tttcggcaac gcgaccggtt taagcttcag tgactttgtc tggtgatttg
    31021 tgagtgtatt taaccgtgcc tcaataattt tcattttacc cgcgacatcg ttgagctgct
    31081 gccgggtttt gctggcatta atatcggttt ccacaccttc aactgaagaa gtaatcccgt
    31141 tctgatatag ctggcgatcg gtcgcgataa tggtgttctg ctctttttcg atttgctgca
    31201 agaccgtgtt taacgccgcc tgggtttgcc actcccagta caggcgggct acgctgccag
    31261 ccagcaattg gcgggtttgc tcgcgttccg ccgcccgtgc tttaaccgta cccaggcgag
    31321 cagtaacctc cgcccgattc tttccccaga tatcgagatg ccagcccgcc gttaagccaa
    31381 aagtaccgtt ggtgtaccac ggaccggtcg tacctgcggc cggatcgttc aaagcaaacg
    31441 gccccattaa cccttctgcc gacatttttt gccgctccat atccgcagaa aagtcgagct
    31501 gcggaccatc ctgagtggca attgccttcg cctgggcttc agccaactga atgcgctgtt
    31561 cagccacctg catatccggt gcgctctgta gtgcgttatt aattaaggag gtgagttgat
    31621 tatcgtgata ctccagccac cattggctgt ctggccaacc atttttcagc gccgtgggta
    31681 atacggtgtc aacttgtgca gcgggcgttt gctggcttaa cgcctggcgg gtttcatgca
    31741 tagtcgcaca cccggccagc atcagtaaca gcggaaaaca ggcgatggcg ggataaaaag
    31801 aatcacgatt catgggggaa taatcaggta agaaaaggtg cgcggagatt accgtgtgtt
    31861 gcgatatatt ttttagtttc gcgtggcaat acatcagtag caataaaacg acatatccag
    31921 aaaaatatac actaagtgaa tgatatcttc cgatttatct taatcgttta tggataacgg
    31981 caaagggctt cgttttttcc tatacttatt cagcactcac aaataaagga acgccaatga
    32041 aaattatact ctgggctgta ttgattattt tcctgattgg gctactggtg gtgactggcg
    32101 tatttaagat gatattttaa aaatcagata tggcatataa gttgtcaggt caggaactag
    32161 atattttgtg tacttgattt gcaaacagct ccggtgttgg tggcgtcaaa atcgctgaag
    32221 cgttccccga aggccggggc agcccacatg gatgtgggct gagggcgcgt cttacaagga
    32281 cgttacctcg cgcccgaccc ggtagccgaa ggggataagc
//
LOCUS       DAZKHK010000049        32229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKHK010000049 DAZKHK010000000
VERSION     DAZKHK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.049"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(62..1822)
                     /gene="ycaO"
                     /locus_tag="ACHOS5_002270"
     CDS             complement(62..1822)
                     /gene="ycaO"
                     /locus_tag="ACHOS5_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP9777083.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKISDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1952..2644
                     /locus_tag="ACHOS5_002271"
     CDS             1952..2644
                     /locus_tag="ACHOS5_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9777084.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2843..3931
                     /gene="serC"
                     /locus_tag="ACHOS5_002272"
     CDS             2843..3931
                     /gene="serC"
                     /locus_tag="ACHOS5_002272"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP9777085.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4002..5285
                     /gene="aroA"
                     /locus_tag="ACHOS5_002273"
     CDS             4002..5285
                     /gene="aroA"
                     /locus_tag="ACHOS5_002273"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9777086.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            5455..6219
                     /gene="ycaL"
                     /locus_tag="ACHOS5_002274"
     CDS             5455..6219
                     /gene="ycaL"
                     /locus_tag="ACHOS5_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP9777087.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6392..7075
                     /gene="cmk"
                     /locus_tag="ACHOS5_002275"
     CDS             6392..7075
                     /gene="cmk"
                     /locus_tag="ACHOS5_002275"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9777088.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7185..8858
                     /gene="rpsA"
                     /locus_tag="ACHOS5_002276"
     CDS             7185..8858
                     /gene="rpsA"
                     /locus_tag="ACHOS5_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9777089.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9018..9302
                     /gene="ihfB"
                     /locus_tag="ACHOS5_002277"
     CDS             9018..9302
                     /gene="ihfB"
                     /locus_tag="ACHOS5_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP9777090.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9508..11772
                     /locus_tag="ACHOS5_002278"
     CDS             9508..11772
                     /locus_tag="ACHOS5_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP9777091.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSLLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11809..13557
                     /gene="msbA"
                     /locus_tag="ACHOS5_002279"
     CDS             11809..13557
                     /gene="msbA"
                     /locus_tag="ACHOS5_002279"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP9777092.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13554..14540
                     /gene="lpxK"
                     /locus_tag="ACHOS5_002280"
     CDS             13554..14540
                     /gene="lpxK"
                     /locus_tag="ACHOS5_002280"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP9777093.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     SIPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGH"
     gene            14577..15809
                     /gene="ycaQ"
                     /locus_tag="ACHOS5_002281"
     CDS             14577..15809
                     /gene="ycaQ"
                     /locus_tag="ACHOS5_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP9777094.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVVRSPYLVLFSRLGDYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIALLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATYSAVLSPFDPVVWDRKRAEQLFDFSYRLECYIPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            15861..16043
                     /gene="ycaR"
                     /locus_tag="ACHOS5_002282"
     CDS             15861..16043
                     /gene="ycaR"
                     /locus_tag="ACHOS5_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP9777095.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16040..16786
                     /gene="kdsB"
                     /locus_tag="ACHOS5_002283"
     CDS             16040..16786
                     /gene="kdsB"
                     /locus_tag="ACHOS5_002283"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP9777096.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLKRVRAEMR"
     gene            16940..17833
                     /locus_tag="ACHOS5_002284"
     CDS             16940..17833
                     /locus_tag="ACHOS5_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP9777097.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWELLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17810..18589)
                     /gene="elyC"
                     /locus_tag="ACHOS5_002285"
     CDS             complement(17810..18589)
                     /gene="elyC"
                     /locus_tag="ACHOS5_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP9777098.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPIADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18725..19510
                     /gene="cmoM"
                     /locus_tag="ACHOS5_002286"
     CDS             18725..19510
                     /gene="cmoM"
                     /locus_tag="ACHOS5_002286"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP9777099.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVTDPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19507..20829
                     /gene="mukF"
                     /locus_tag="ACHOS5_002287"
     CDS             19507..20829
                     /gene="mukF"
                     /locus_tag="ACHOS5_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP9777100.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20810..21514
                     /gene="mukE"
                     /locus_tag="ACHOS5_002288"
     CDS             20810..21514
                     /gene="mukE"
                     /locus_tag="ACHOS5_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP9777101.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21514..25974
                     /gene="mukB"
                     /locus_tag="ACHOS5_002289"
     CDS             21514..25974
                     /gene="mukB"
                     /locus_tag="ACHOS5_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP9777102.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26235..28082
                     /gene="ldtD"
                     /locus_tag="ACHOS5_002290"
     CDS             26235..28082
                     /gene="ldtD"
                     /locus_tag="ACHOS5_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9777103.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMTRDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28263..28811
                     /locus_tag="ACHOS5_002291"
     CDS             28263..28811
                     /locus_tag="ACHOS5_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP9777104.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28838..29485
                     /gene="gloC"
                     /locus_tag="ACHOS5_002292"
     CDS             28838..29485
                     /gene="gloC"
                     /locus_tag="ACHOS5_002292"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP9777105.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFLDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29537..30727)
                     /gene="aspC"
                     /locus_tag="ACHOS5_002293"
     CDS             complement(29537..30727)
                     /gene="aspC"
                     /locus_tag="ACHOS5_002293"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP9777106.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30912..32000)
                     /gene="ompF"
                     /locus_tag="ACHOS5_002294"
     CDS             complement(30912..32000)
                     /gene="ompF"
                     /locus_tag="ACHOS5_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP9777107.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7881 a         7828 c         8646 g         7874 t
ORIGIN      
        1 aaacgccccg taatataacc cagacgaatc attaagccta cattgcgtag gctatttgat
       61 tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc ttcaacaacg
      121 actgatgtgc agcaaacgcg tgcagatcgc tatctactgg ttgcaagcca taaaacgccg
      181 cttcgccgct cattgccgca ctggcggctt ccacggcatc tgcgccgtac atgcgaacaa
      241 acgcattcag atattgcagc ggctggcgat cttcttcctg cgccagtaat aacaacgttt
      301 gcaggcagcg ataataattg gcgcgttccg ggctgaatac tgatgagtta aactccatcg
      361 tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc agcatggctt
      421 ttaattcacc gatacgcaga gtgtaccagc cgttatccga cccggtcgcc aggcccaaca
      481 gttcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg atgaggttca
      541 ggtaatcttc cttttcccac tcgctacctg gtagcgaaag aatcgtttca cgtaaatggc
      601 tgcccatact gttattcgcc agccacagat cttcagccgg ataaatatcg gacatgccag
      661 gtacgataat acggcaagcg tagacaccca gatgctcgta atcggcaata taaacttctt
      721 tatcttcctt tttgaagata gccatcagcg tggcgaactc ttcttccgtg gtgccggaga
      781 agctccagtc aacaaacgga taatcggcat cctgcttgaa caggtcccag gagattaaac
      841 cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct tcatcatcga
      901 aggttggtgg agtaaacaca tccagatctt tcaggccacg accttgcagc aactcggtca
      961 cggtacgttc cagtgcaacg ccaaaatcag gatgcgcacc gaatgaggca aagcaggtac
     1021 cgttagcagg attgaacagt accacgcaaa tcaccggata ctggccgcca agcgaaccgt
     1081 cataagcgaa gatcgggaaa ccttccgctt ccagcgtttc gatcgcttca actactgctg
     1141 gataacgtgc cagcacgtct gccgggattt ccggcaagct gatgctttca gcaataatgc
     1201 ggtttttcac gtaacgttcg aaaacttcgg acaacccctg aacgcgtgct tcgttacggg
     1261 tattgccagc agacataccg ttagaaacgt acaagttacc aatgatattc atcggaatat
     1321 aaatggtctg attgtcggac tggcgcgtaa acggtagacc gcaaatacca cgatcttcgt
     1381 taccggattg caggtcaatc agcatactac cggtcagttc attctccgga tcgtaaaatg
     1441 cgcgcagacg gtcatcgagc agcccttctg gtacatcgtc attttcggtc agtgggaacc
     1501 atttttcgtt gggataatgc acgaatgggc cgttggcgat ggtttcgccc agccagaagt
     1561 ccgcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagggca gaagccagcg
     1621 ccgctttttt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct ttgtcgcgaa
     1681 tatgtacaga ccagacatta ggcacgggat tcagccagga ggcctcttca atctgaaaac
     1741 cgaggtctga aattttttgc tggaagcgtg cgatggaatc ttccagagcg gcatctttgc
     1801 cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg cgcaataata
     1861 cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt agctatgctt
     1921 ttagcggatt aactacgata agagcataaa aatgaaggcg tttgatctcc accgtatggc
     1981 attcgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc tttatacctt
     2041 tgtactggtc tttttgttcc tcaaaatgac cggaagacgc ggtgtgcggc agatgtcgct
     2101 gtttgaagtg ttaatcattc tgacactggg ttcagcggcg ggagatgtgg cgttttatga
     2161 tgatgtgccg atggtcccgg tacttatcgt ctttattact ctggcgttgt tataccgcct
     2221 ggtaatgtgg ttgatggcgc acagtgaaaa actggaagat cttctggaag gcaagccggt
     2281 tgtcattatt gaagatggcg agctggcctg gtcgaaactc aataactcca acatgacgga
     2341 atttgagttc tttatggagc tacgattgcg cggtgtagag cagttggggc aggtacgtct
     2401 ggcgattctc gaaaccaacg ggcaaatcag tgtttatttc tttgaagatg acaaggtgaa
     2461 accggggtta cttattttac ccagtgactg tactcagcgt tacaaagtgg tgccggagtc
     2521 ggcggactat gcctgcatcc gttgcagtga aatcattcat atgaacgcgg gggaaaaaca
     2581 attatgtccg cgctgtgcaa atccagaatg gacgaaggca agtcgggcta aacgggtgac
     2641 ctgacagcca aaacatcggt tttttgaaaa taatccgaga gattcttttg tgtgacgcgt
     2701 gcctcatttt tgccctcaac ggttttactc attgcgatgc gtgtcactga atgataaaac
     2761 cgatagccac aggaataatg tattgcctgt ggtcgcaatt gattgaccgc gggttaatgg
     2821 caacgcaacg tggtgagggg aaatggctca aatcttcaat tttagttctg gtccggcaat
     2881 gctaccggca gaggtgctta aacaggctca acaggaactg cgcgactgga acggtcttgg
     2941 tacgtcggtg atggaagtga gtcaccgtgg caaagagttc attcaggttg cagaggaagc
     3001 cgagaaggat tttcgcgatc ttcttaacgt cccctccaac tacaaggtat tgttctgcca
     3061 tggcggtggt cgcggtcaat ttgctgctgt accgctgaat attcttggtg ataaaaccac
     3121 tgcagattat gttgatgcgg gttactgggc agcaagtgct atcaaagaag cgaaaaaata
     3181 ctgcacacca aatgtgtttg atgcgaaagt gaccgttgat ggtcttcgcg cagttaagcc
     3241 aatgagtgaa tggcaactct ctgataatgc tgcttatatg cattactgcc cgaacgaaac
     3301 cattgacggt atcgctatcg atgaaacacc agacttcggt aaagacgtgg tggtcgccgc
     3361 tgacttctct tcaaccattc tttcccgtcc gattgatgtc agccgttatg gtgtaattta
     3421 cgctggcgcg cagaaaaata tcggcccggc tggcctgaca atcgtcatcg ttcgtgaaga
     3481 tttgctgggc aaagcgaata tcgcctgccc atcgattctg gattactcca tcctcaacga
     3541 taacgattcc atgttcaaca cgccgccgac atttgcctgg tacttatctg gcctggtctt
     3601 caaatggctg aaagcgaacg gcggtgtggc tgcaatggat aagatcaatc agcaaaaagc
     3661 agaactgctg tatggggtga ttgataacag cgatttctac cgcaatgatg tggcgaaagc
     3721 taaccgttcg cggatgaacg tgccgttcca gttggcggat agcgcgcttg ataaattgtt
     3781 cctcgaagag tctttcgctg cgggcttaca tgcgctgaaa ggtcaccgag tggtcggcgg
     3841 aatgcgtgcg tctatttata acgccatgcc gctggaaggt gttaaagcac tgacagactt
     3901 catggttgag ttcgaacgtc gtcacggcta atgtcgaaat tttgcataat ccccacagcc
     3961 agcctgtggg gtttttattt ctgttgtaga gagttgagtt catggaatcc ctaacgttac
     4021 aacccatcgc tcgtgtcgat ggcactatta atctgcccgg ttccaagagc gtttctaacc
     4081 gcgctttatt gctggcggca ttagcacacg gcaaaacagt attaaccaat ctgctggata
     4141 gcgatgacgt gcgccatatg ctgaatgcat taacagcgtt aggggtaagc tatacgcttt
     4201 cagccgatcg tacgcgttgc gaaattatcg gtaacggtgg tccattacac gcagaaagtg
     4261 cccgggagtt gttcctcggt aacgccggaa cggcaatgcg tccgctggcg gcagcacttt
     4321 gcctgggtag caatgatatt gtactgaccg gtgaaccgcg aatgaaagaa cgcccgattg
     4381 gtcatctggt ggatgcgctg cgccagggcg gggcgaagat cacttacctg gaacaagaaa
     4441 attatccgcc attgcgttta cagggcggct ttactggcgg caacgttgat gttgatggct
     4501 ccgtttccag ccagttcctc accgcactgt taatgactgc gcctcttgcg ccggaagata
     4561 cggtgattcg tattaaaggc gatctggttt ctaaacctta tatcgatatc accctcaatc
     4621 tgatgaagac gtttggtgtt gaaattgaaa atcagcacta tcaacaattt gtcgtaaaag
     4681 gggggcagtc ttaccagtct ccgggcactt atttggttga aggcgatgca tcttcggctt
     4741 cttatttcct ggcagcagca gcaatcagag gcggcactgt aaaagtgacc ggcattggac
     4801 gcaacagtat gcagggtgat attcgctttg ctgatgttct ggaaaaaatg ggcgcgacca
     4861 tttgctgggg cgatgattat atttcctgca cgcgtggtga actgaacgcc attgatatgg
     4921 atatgaacca tattcccgat gcggcgatga ccattgccac cgcggcgtta tttgcaaaag
     4981 gcaccaccac gctgcgcaat atctataact ggcgtgttaa agagaccgat cgcctgtttg
     5041 cgatggcaac agaactgcgt aaagtcggtg cggaagtcga agaggggcat gatttcattc
     5101 gcattacgcc accggaaaaa ctgaaatttg ctgagatcgc cacttataat gatcaccgga
     5161 tggcgatgtg tttctcgctg gtcgcattat cagatacgcc tgtgacgatt cttgatccaa
     5221 aatgtaccgc taaaactttt ccagactatt tcgagcaatt ggcgcggata agtcaacccg
     5281 gctgaatgaa taacgggcga taacatagac aatcctttct tttcaaagac gtcggcacat
     5341 tgccggcgtt ttttttcgga ccttgtgggg tatttttatt aatggtagcg ttaattatca
     5401 atgtaacttg ttgatacata atgtttatat ataattaatc aacggatgat tcacatgaag
     5461 aatactaaat tattgctggc gattgcgacc tctgcagcct tactgacagg atgtcagaat
     5521 acgcacggta ttaataccga tttggctatc agctcaggtc tgaatgctta taaagccgca
     5581 acattaagcg atgctgatgc taaagcaatc gccaatcagg gctgtgtcga aatggacagc
     5641 ggcaataaag tggcaagcaa atccagcaag tacgggaaac gtctggcaaa aatcgccaaa
     5701 gcattgggta acaatattaa tggcacgcca gtcaactata aggtttatat gaccagcgac
     5761 gtcaacgcat gggcgatggc gaacggttgt gttcgtgtct acagtggcct gatggacatg
     5821 atgaacgaca acgaaattga aggcgttctg ggccatgaac tgggccacgt cgcgttgggt
     5881 cactcgctgg ctgaaatgaa agcttcttat gcgatcgtgg ccgcacgcga tgccatttca
     5941 gctaccagcg gtgtggcttc ccagctttcc cgttcacaat taggtgatat cgcagaaggc
     6001 gctatcaatg ctaaatactc ccgtgataaa gaatccgaag cagatgattt ctcttttgat
     6061 ctgttgaaga aacgtggcat cagtacccag gggctggttg gtagctttga aaaactggcc
     6121 agcctggatg gcggtcgcac ccagtccatg tttgattctc acccaccatc aacagagcgt
     6181 gcgcaacaca tccgtgatcg tatcgcctct ggtaagtaaa tcattgtcat ctttcgggct
     6241 ggtcttctgc cagcccgcta taattgcgca ataaatcccc atctgaatac agacaaaact
     6301 ggtttttgca cacaacgtta acgatttgtg gcgtcggcgc gtataatgcg cgcggttatg
     6361 ttaacggtac gcctgtttta aggagataaa gatgacggca attgccccgg ttattaccat
     6421 tgatggccca agcggtgcag ggaaaggcac cttgtgtaag gctatggcgg aagcgttgca
     6481 atggcatctg ctggattcgg gtgcaattta tcgcgtactg gcactagcgg cattacatca
     6541 ccatgttgat gttgcatcgg aagatgcgct ggtgccgctg gcatcccatc tggatgtacg
     6601 ttttgtgtca accaacggta atctggaagt gatcctcgaa ggggaagatg tcagcggcga
     6661 aattcgtact caggaagtgg cgaatgctgc ttcacaagtc gcggcattcc cacgcgttcg
     6721 tgaagcatta ttgcgtcgcc aacgcgcgtt tcgcgaatta ccaggcctga ttgccgatgg
     6781 ccgcgacatg ggaacggtgg tattccctga tgcgccagtg aaaattttcc ttgacgcctc
     6841 ctcggaagaa cgtgcgcatc gccgcatgct acagttgcag gagaagggct ttagtgttaa
     6901 ctttgagcgc cttttggccg agatcaaaga acgcgacgac cgcgatcgta accgagcggt
     6961 agcgccactg gttccggcag ccgatgcttt agtgttggat tccaccacct taagcattga
     7021 gcaagtgatt gaaaaagcgc tacaatacgc gcgccagaaa ttggctctcg cataagcgac
     7081 cgatttgcag tacccccgtt gcaatggaat gacagcgggt atgttaaaca accccatccg
     7141 gcatggagcc aggtggacgt taaatataaa cctgaagatt aaacatgact gaatcttttg
     7201 ctcaactctt tgaagagtcc ttaaaagaaa tcgaaacccg cccgggttct atcgttcgtg
     7261 gcgttgttgt tgctatcgac aaagacgtag tactggttga cgctggtctg aaatctgagt
     7321 ccgccatccc ggctgagcag ttcaaaaacg cccagggcga gctggaaatc caggtaggtg
     7381 acgaagttga cgttgctctg gacgcagtag aagacggctt cggtgaaact ctgctgtccc
     7441 gtgagaaagc taaacgtcac gaagcctgga tcacgctgga aaaagcttac gaagatgctg
     7501 aaactgttac cggtgttatc aacggcaaag ttaagggcgg cttcactgtt gagctgaacg
     7561 gtattcgtgc gttcctgcca ggttctctgg tagacgttcg tccggtgcgt gacactctgc
     7621 acctggaagg caaagagctt gaattcaaag taatcaagct ggatcagaag cgcaacaacg
     7681 ttgttgtttc tcgtcgtgcc gttatcgaat ccgaaaacag cgcagagcgc gatcagctgc
     7741 tggaaaacct gcaggaaggc atggaagtta aaggtatcgt taagaacctc actgactacg
     7801 gtgcattcgt tgatctgggc ggcgttgacg gcctgctgca catcactgac atggcctgga
     7861 aacgcgttaa gcatccgagc gaaatcgtca acgtgggcga cgaaatcact gttaaagtgc
     7921 tgaagttcga ccgcgaacgt acccgtgtat ccctgggcct gaaacagctg ggcgaagatc
     7981 cgtgggtagc tatcgctaaa cgttatccgg aaggtaccaa actgactggt cgcgtgacca
     8041 acctgaccga ctacggctgc ttcgttgaaa tcgaagaagg cgttgaaggc ctggtacacg
     8101 tttccgaaat ggattggacc aacaaaaaca tccacccgtc caaagttgtt aacgttggcg
     8161 atgtagtgga agttatggtt ctggatatcg acgaagaacg tcgtcgtatc tccctgggtc
     8221 tgaaacagtg caaagctaac ccgtggcagc agttcgcgga aacccacaac aagggcgacc
     8281 gtgttgaagg taaaatcaag tctatcactg acttcggtat cttcatcggc ctggacggcg
     8341 gcatcgacgg cctggttcac ctgtctgaca tctcctggaa cgttgcaggc gaagaagcag
     8401 ttcgtgaata caaaaaaggc gacgaaatcg ctgcagttgt tctgcaggtt gacgcagaac
     8461 gtgaacgtat ctccctgggc gttaaacagc tcgcagaaga tccgttcaac aactgggttg
     8521 ctctgaacaa gaaaggcgct atcgtaaccg gtaaagtaac tgcagttgac gctaaaggcg
     8581 caaccgtaga actggctgac ggcgttgaag gttacctgcg tgcttctgaa gcatcccgtg
     8641 accgcgttga agacgctacc ctggttctga gcgttggcga cgaagttgaa gctaaattca
     8701 ccggtgttga tcgtaaaaac cgcgcaatca gcctgtctgt tcgtgcgaaa gacgaagctg
     8761 acgagaaaga tgcaatcgca actgttaaca aacaggaaga tgcaaacttc tccaacaacg
     8821 caatggctga agctttcaaa gcagctaaag gcgagtaatt ctctgactct tcgggatttt
     8881 tattccgaag tttgttgagt ttacttgaca gattgcaggt ttcgtcctgt aatcaagcac
     8941 taagggcggc tacggccgcc cttaatcaat gcagcaacag cagccgctta atttgccttt
     9001 aaggaaccgg aggaatcatg accaagtcag aattgataga aagacttgcc acccagcaat
     9061 cgcacattcc cgccaagacg gttgaagatg cagtaaaaga gatgctggag catatggcct
     9121 cgactcttgc gcagggcgag cgtattgaaa tccgcggttt cggcagtttc tctttgcact
     9181 accgcgcacc acgtaccgga cgtaatccga agactggcga taaagtagaa ctggaaggaa
     9241 aatacgttcc tcactttaaa ccaggtaaag aactgcgcga tcgcgccaat atttacggtt
     9301 aagtttttac tcaaacttga acgcgagaaa agcacctgtc gggtgctttt ttcatttctc
     9361 taatctggaa ctggaagctg cctcgcagag ttttgagcag ttttcaccct atcgttaaat
     9421 tcttctgaac gtgcctcggg gaacgcaaaa ctcccaccag acaaccgttc aacaaagttg
     9481 cacactttct ataaacaggg agggtgcatg aaaataacga cagtcggcgt atgcataatt
     9541 tgcggaattt ttccgttgct gattttgccc caattgccag ggacagtaac ccttgcgttt
     9601 ctgactctct tcgcctgtgt actggcattt atccctgtta aaaccgtccg ttatatcgcg
     9661 ctgacgttgc tgtttttcgt ttggggcata ttagcagcaa agcaaatttt gtgggcagga
     9721 gaaaccttaa ctggcgcgac gcaggatgca attgttgaga tcattgctac tgacggcatg
     9781 accactcatt acggtcaaat tactcatcta caaggtcgac gtatattccc tgcgccaggc
     9841 ctcgtactgt atggcgaata tcttccgcaa gcggtttgtg ccggacaagt atggtcaatg
     9901 aaactcaaag ttcgtgcagt tcatggtcaa cttaatgatg gcggctttga tagccagcgt
     9961 tatgccattg cccagcatca accgctcacc ggccgttttc tgcaggcaag tgtcattgaa
    10021 ccgaattgta gcctgcgtgc acagtatctg gcgtcactac aaacaacgct gcaaccctat
    10081 atgtggaacg cggttattct tggattaggt atgggggaac ggttatccgt ccccaaagaa
    10141 atcaaaaata tcatgcgtga tactggaacg gcgcatttaa tggcgatatc aggattgcac
    10201 atcgcttttg cggcgttgct ggctgcagga ctcattcgcg gtgggcaagt ttttctgcct
    10261 gggtgctgga tccactggca aatgccatta attggtggaa tctgctgtgc tgctttttat
    10321 gcctggctta cgggaatgca acctcctgca ttgcgtaccg tggtggcgct tgccatttgg
    10381 ggaatgctta agttaagtgg acgacaatgg agtggctggg atgtatggat atgttgtctg
    10441 gcggcaattt tgctgatgga tcctgttgcc attctctcgc aaagtttatg gctatctgcc
    10501 gctgcggtcg cggcactgat tttttggtat cagtggtttc cctgtccgga gtggcagctg
    10561 ccgccggtat tgcgcgcggt tgtttccctc atccatctgc aactgggaat caccctcctg
    10621 cttatgcccg tgcaaattgt catatttcat ggcattagtc tgacctcgtt tattgcaaat
    10681 ctttttgcaa ttcccctggt gacatttatc acggttccgt tgattctcgc cgcgatggtt
    10741 gtgcatttaa gcgggccgtt tattctggaa caagggttat ggtttcttgc cgaccggtct
    10801 ttggctttac ttttctgggg gttaaagagt ttgccggaag ggtggatcaa cattgctgaa
    10861 cgttggcaat ggctaacatt ttccccatgg ttcttactgg tggtatggcg attaaacgcc
    10921 tggcgaacgt tgccagcaat gtgtgtggct gtaggcttgc tgatgtgctt gccgctgtgg
    10981 caaaaacctc gacctgacga gtggcaagtg tacatgcttg atgtcgggca agggctggca
    11041 atggtcattg cgcgaaacgg caaagcgatt ctctatgaca cgggactggc ctggcccgaa
    11101 ggggatagtg ggcaacaact gattatcccc tggctccact ggcataatct tgaaccggaa
    11161 ggcgttattt tgagtcatga acatctggat caccggggag ggctggactc aatattgcac
    11221 acatggccga tgttatggat ccgaagtccg ttaaactggg aacatcatca gccctgtgtg
    11281 cgtggcgaag cgtggcaatg gcaaggattg cgtttcagcg cgcactggcc tttacaaggt
    11341 agcaacgata aaggaaataa ccattcctgt gtggttaaga ttgatgacgg gacgaatagc
    11401 attcttctaa ccggtgatat tgaagccccc gctgaacaaa agatgctaag ccgttactgg
    11461 cagcaaatgc aggcaacatt acttcaggta cctcaccatg gcagtaatac ctcatcattg
    11521 ttgccattaa ttcagcgagt gaatggaaaa gtggcactcg catcggcatc gcgctataac
    11581 gcatggcgac tgccctcaag caaagttaaa catcgctatc aacaacaggg ttataagtgg
    11641 cttgataccc cacatcaggg tcaaataacg gtcaattttt cagcgcaagg ctggcgaatt
    11701 agcagcctca gagagcaaat tttacctcgt tggtatcatc agtggtttgg cgtgccagtg
    11761 gataacgggt agaatatgcg gctatttcaa caaatgctgg ttttttgaat gcataacgac
    11821 aaagatctct ctacgtggca gacgttccgc cgactgtggc caaccattgc gccttttaaa
    11881 gcgggtctga tcgtggcggg cgtagcgtta atcctcaacg cagccagcga taccttcatg
    11941 ttatcgctcc ttaagccact tcttgatgat ggctttggta aaacagatcg ctccgtgctg
    12001 atgtggatgc cgctggtggt gatcgggctg atgattttac gtggtatcac cagctatatc
    12061 tccagctact gtatctcctg ggtatcagga aaggtggtaa tgactatgcg tcgccgcctg
    12121 tttggtcaca tgatgggaat gccggtttca ttctttgaca aacagtcaac gggtacgctg
    12181 ttgtcacgta tcacctacga ttccgaacag gtcgcttctt cttcttccgg cgcactgatt
    12241 actgttgtgc gtgaaggtgc gtcgatcatc ggcctgttca tcatgatgtt ctattacagt
    12301 tggcaactgt cgatcatttt gattgtgctg gcaccgattg tttcgattgc gattcgcgtt
    12361 gtatcgaagc gttttcgcaa catcagtaaa aacatgcaga acaccatggg gcaggtgacc
    12421 accagcgccg aacaaatgct gaaaggccat aaagaagtat tgattttcgg tggtcaggaa
    12481 gtggaaacga aacgcttcga taaagtcagc aaccgaatgc gtcttcaggg gatgaaaatg
    12541 gtttcagcct cttccatctc tgatccgatc attcagctga tcgcctcttt ggcgctggcg
    12601 tttgttctgt atgcggcaag cttcccaagt gtcatggata gcctgactgc cggtacgatt
    12661 accgttgttt tctcttcaat gattgcactt atgcgtccgc tgaaatcgct gaccaacgtt
    12721 aacgcccagt tccagcgcgg tatggcggct tgtcagacgc tgtttaccat tctggacagt
    12781 gagcaggaga aagacgaagg taagcgcgtg atcgagcgtg cgactggcga cgtggagttt
    12841 cgcaatgtca cctttactta tccgggacgt gatgtgcccg cattgcgtaa catcaacctg
    12901 aaaattccgg cagggaagac ggttgctctg gttggacgat ctggttcagg taaatcaacc
    12961 atcgccagcc taatcacacg tttttacgat attgatgaag gcgaaatcct gatggatggt
    13021 cacgatctgc gcgagtatac cctggcgtcg ttacgtaacc aggttgctct ggtgtcgcag
    13081 aatgtccatc tgtttaacga tacggttgcc aacaacattg cttacgcgag gactgaacag
    13141 tacagccgtg agcaaattga agaagctgcg cgtatggcct acgccatgga cttcatcaat
    13201 aagatggata acggtctcga tacagtcatt ggtgaaaacg gcgtgttgct ctctggcggt
    13261 cagcgtcagc gtattgctat cgctcgagcc ttgttgcgtg atagcccgat actgattctg
    13321 gacgaagcta cctcggcgtt ggataccgaa tccgaacgtg cgattcaggc ggcactggat
    13381 gagttgcaga aaaaccgtac ctctctggtg attgcccacc gcttgtctac cattgaaaag
    13441 gcagacgaaa tcgtggttgt cgaggatggt gtcattgtgg aacgcggtac gcataacgat
    13501 ttgcttgagc accgtggcgt ttacgcgcaa cttcacaaaa tgcagtttgg ccaatgatcg
    13561 aaaaaatctg gtctggtgaa tcccctttat ggcggctatt gctgccactc tcctggttgt
    13621 atggcctggt gagtggcgcg atccgtcttt gctataaact aaaactgaag cgcgcctggc
    13681 gtgcctccat accggttgtc gtggttggta atctcaccgc aggcggcaac ggaaaaaccc
    13741 cggtcgttgt ctggctggtg gaacagctgc aacagcgcgg tattcgcgtg ggggttgtat
    13801 cgcggggata tggtggtaag gctgaatctt atccgctgtt attgtcggca gataccacca
    13861 cagcacaggc gggtgatgaa cctgtgttga tttatcaacg cactggcgca cctgttgcgg
    13921 tttctcccgt gcgttctgac gcggtaaaag ccattctggc gcaacaccct gatgtgcaga
    13981 tcatcgtaac cgacgatggt ttacagcatt atcgtctggc gcgtgatgtg gaaattgtcg
    14041 ttattgatgg tgtgcgtcgc tttggcaatg gctggtggtt gccggcgggg ccaatgcgtg
    14101 agcgagcggg gcgcttaaag tcggttgatg cggtaatcgt caacggcggt gtcccccgca
    14161 gcggtgaaat ccccatgcat ctgctgccgg gtcaggcggt gaatttacgt accggtacgc
    14221 gttgtgacgt tgctcagctt gaacatgtgg tggcgattgc ggggattggg catccgccgc
    14281 gcttttttgc cacgctgaag atgtgcggcg tacaaccgga aaaatgtgta ccgctggccg
    14341 atcatcagtc tttgaatcat gcggatgtca gcgcgttggt aagcgccgga caaacgctgg
    14401 taatgactga aaaagatgcg gtgaaatgcc gggcctttgc agaagaaaat tggtggtatt
    14461 tgcctgtaga cgcacagctt tcaggtgatg aaccagcgaa actgcttgcg caactaacct
    14521 cgctggcttc tggccactag ttacgccgcg gcagcgttcg attgatggag tcatgaatgt
    14581 cgctgccgca cctctccctt gctgatgcgc gtaatcttca ccttgccgcg caaggcctgt
    14641 taaacaaacc ccgccgtcga gcgtcgttgg aggatattcc ggcaacgatc tcccgcatgt
    14701 ccttgctgca aatcgatacc atcaatattg ttgtccgtag cccatatctg gtgcttttca
    14761 gccgtctggg agattatcct gcgcagtggc tggatgagtc tctggcgcgt ggagaattaa
    14821 tggaatactg ggcgcatgaa gcctgcttta tgccgcgtag cgattttcgt cttattcgcc
    14881 accgcatgct ggcacctgaa aaaatgggct ggaaatataa agacgcctgg atgcaggaac
    14941 atgcggcgga aattgcactg ttaattcagc atattcatga taaggggccg gtacgttcag
    15001 ccgattttga gcatcctcgt aaaggggcga gcggctggtg ggagtggaag ccgcataaac
    15061 gacatctgga aggtttattt actgccggaa aggtgatggt gattgaacgg cgcaacttcc
    15121 agcgcgttta tgatttaact caccgtgtca tgcctgactg ggatgatgag cgcgatctcg
    15181 tttcgcaagc agaagcagaa atcatcatgc tggataacag tgcgcgtagc ctgggaatat
    15241 ttcgcgaaca gtggctggca gattactacc ggctaaaacg tccggcactg gcggcgtggc
    15301 gtgaagcgag ggctgaacag cagcaaatca ttgctgtgca tgttgaaaaa ttgggtaatc
    15361 tttggctgca tgctgatttg ctgccgctac tcgagcgtgc gctcgccgga aagctcactg
    15421 caacgtacag cgcggtactt tcgccttttg atcctgttgt ctgggatcgc aaacgcgcgg
    15481 agcagctttt tgattttagc taccggctgg agtgctatat cccagcgccg aaacgccagt
    15541 atggctattt tgttctgccg ttattacatc gtgggcaatt agttgggcga atggatgcca
    15601 aaatgcatcg ccagacaggc atccttgaag ttatctctct gtggttacag gaaggcatta
    15661 aaccaacgac aacgctgcaa aaagggttac gtcaggcgat tactgatttc gctaactggc
    15721 agcaggcaac gcgggtgaca ttaggacgct gcccgcaagg cctctttact gattgccgcg
    15781 ccggctggga aatagacccc gtcgcataaa tgattatgat aagatttatg cattcataac
    15841 ccgatccatc tggaggaact atggatcatc gtctgcttga aatcattgcc tgcccggttt
    15901 gcaacggaaa actttggtat aaccaggaaa aacaagagct tatttgcaaa ctggacaacc
    15961 tggctttccc gctgcgcgat ggcattccag tgttgctgga aacggaagcc cgcgtgctga
    16021 ctgctgatga gagtaaatca tgagttttgt ggtcattatt cccgcgcgtt acgcgtccac
    16081 gcgtctgccc ggtaaaccat tggttgatat taacggcaaa cccatgattg ttcatgttct
    16141 tgaacgcgcg cgcgaatcag gtgccgagcg catcatcgtg gcaaccgatc atgaggatgt
    16201 tgcccgcgct gttgaagccg ctggcggcga agtatgtatg acgcgcgccg atcatcagtc
    16261 aggtacagaa cgattggcgg aagttgtcga aaaatgcgca ttcagcgacg acactgtgat
    16321 cgttaatgtg cagggtgatg aaccgatgat ccctgcgaca atcattcgtc aggttgctga
    16381 taacctcgct cagcgtcagg tgggtatggc gactctggcg gtgccaatcc acaatgcgga
    16441 agaagcgttt aacccgaatg cggtgaaagt ggttctcgac gccgaagggt acgcactgta
    16501 cttctctcgc gccaccattc cgtgggatcg tgatcgtttt gcaaaagacc ttgaaaccgt
    16561 aggcgataac ttcctgcgtc atcttggtat ttatggctac cgagcaggct ttatccgtcg
    16621 ttatgttacc tggcagccaa gtccgttaga acatatcgaa atgttagagc agcttcgtgt
    16681 tctgtggtac ggcgaaaaaa tccacgttgc tgtcgcccag gaagttcctg gcacaggtgt
    16741 ggatacccct gaagatctca agcgcgttcg cgccgaaatg cgctaatttc acttcacgac
    16801 actccagcca attttgggag gagtgtcgta ccgttacgat tttcctcaat ttttcttttc
    16861 aacaattgat ctcattcagg tgacatcttt tatattggcg ctcattatga aagcagtagc
    16921 ttttatgagg gtaatctgaa tggaacagct gcgtgccgaa ttaagccatt tactgggcga
    16981 aaaactcagt cgtattgagt gcgtcaatga aaaagcggat acggcgttgt gggctttgta
    17041 tgacagccag ggaaacccaa tgccgttaat ggcaagaagc tttagtacgc ccggaaaagc
    17101 ccgacaactg gcatggaaaa ccaccatgct ggcaagaagt gggactgtcc gtatgccgac
    17161 tatttatggt gtgatgacgc atgaagaaca ccccggcccc gatgtcctgc tactggagcg
    17221 gatgcgtggt gtttcggtgg aggcaccagc ccgaacacca gaacgctggg aactactcaa
    17281 agatcaaatc gttgaagcct tactggcctg gcaccgtcag gacagtcgcg gttgcgtcgg
    17341 cgcggtcgac aatactcagg aaaatttctg gccctcatgg taccggcaac atgttgaagt
    17401 gctatggacc acgctcaatc agttcaataa caccggtctg acgatgcagg ataagcggat
    17461 cctgtttcgt actcgcgaat gtctcccggc attatttgaa ggctttaacg acaattgtgt
    17521 gctgattcac ggtaacttct gtttacgcag catgctgaaa gattcgcgca gcgatcagtt
    17581 actggcgatg gtcgggccgg gactaatgct ttgggcaccg cgagaatacg aactgttccg
    17641 actaatggat aattctctgg cggaagattt gctctggagt tacctgcaac gcgcgccagt
    17701 ggcggagtca ttcatctggc ggcgttggtt gtatgtgtta tgggatgaag ttgcgcaact
    17761 ggttaatacc ggacgattta gtcggcgcaa cttcgatctg gcatcaaaat cactcttgcc
    17821 gtggctcgcc tgacgaacct tttagccact gccagatgcg gccaagcgtt tcatagccaa
    17881 cacgatcgct atgcatcaac caaaccggag aagggattgc ccgttcccac ggattaagcg
    17941 gcgagtcgat ggccaactga ttcgccgggg ctggcagcgg atttagccct tcctgctgaa
    18001 aaaagatcat cgcccgcggc agatgtgatg cggaggtcac cagcaggaaa ggggcgtcac
    18061 caatcgcctg tttcactgct gcagcttctt cttcggtatc ttttggcaaa tccagggtga
    18121 taatttgctc gcgcggcacg cccagcgatt gcgcaactct ggcacctact tcggctgtgc
    18181 tcaccgtatt ggttttagct acgcctcccg taaagatcag ttttgatccc ggattttcgc
    18241 gccataagcg aataccttca ttcaggcgtg gcagactgtt attgattaaa ttagagctcg
    18301 gcgcccactg cgggttccag gtataaccac ctccgagcac cacgatatag tccacttttt
    18361 gcgaattatt ccatgtcgga taagtgcttt cgatgggacg caacaggcgg tcggctatcg
    18421 gttgcaaact caataacaag agcgccagcc acccaatgct aataaaaatc ttcccggttt
    18481 tttgaaaacg gctaaaccac agcagcgcca ggccagcgcc aataatcaac aacatcagcg
    18541 gaagtggcag caacatattg ccaatcactt ttttcagtgt aaaaagcatc ctttctggtt
    18601 ccttttttga ccatatagca agggatcgcc agcgatatta caccagacgg gttcattctc
    18661 gccgtggctg tgacaaaata gcgtctttat cagaaaacgt cttagccaac cggtggagaa
    18721 agcaatgcag gatcgcaatt ttgatgatat tgcggaaaag ttttcccgta acatttacgg
    18781 caccaccaaa gggcagcttc gacaggctat tctgtggcag gatctcgatc gcgtgctggc
    18841 ggaaatgggc ccgcaaaaac tgcgtgtgct ggatgctggc ggcggagaag ggcagaccgc
    18901 aatcaaaatg gccgagcgcg ggcatcaggt cattttatgc gatctttctg cgcagatgat
    18961 cgaccgtgca aaacaggcgg cagaagcaaa aggtgtgagc gacaacatgc aatttataca
    19021 ttgcgccgct caggatgttg cttcgcattt ggaaacgccc gttgatctga tattgttcca
    19081 tgcggtgctc gagtgggtga ccgatccccg cagcgtatta cagaccctgt ggtcagtgtt
    19141 gcgtccaggc ggcgtgttgt cgttaatgtt ctacaatgcg catggtttgt tgatgcataa
    19201 catggtcgcc gggaattttg attacgtgca ggcgggaatg ccgaaaaaga aaaaacggac
    19261 gctttcgcca gattatccac gcgacccggc gcaggtttat ctgtggctgg aagaagctgg
    19321 ttggcaaatt atgggtaaga caggcgttcg cgtgtttcat gattatctgc gcgagaagca
    19381 ccagcagcgc gactgctatg aagcattact tgaattagaa acgcgttatt gccgtcagga
    19441 accgtatatt accctggggc gttatattca tgtcaccgcg cgcaaaccgc agagcaagga
    19501 taaagtatga gtgaattttc ccagacagtc cccgaactgg ttgcctgggc cagaaaaaat
    19561 gacttctcca tctcgctgcc ggtagaccga ctctcttttc tgctggcggt tgccacgctg
    19621 aacggcgagc gtctggatgg tgagatgagt gaaggcgagc tggtggatgc attccgccat
    19681 gtgagtgatg cgtttgagca aaccagcgaa accatcggcg tgcgcgccaa caacgcgatc
    19741 aacgacatgg tgcgtcaacg tctgctgaac cgctttacca gcgagcaggc ggaagggaac
    19801 gcaatttacc gtctgacgcc gctcggcatc ggcattactg actactacat ccgtcagcgc
    19861 gagttttcta cgctgcgcct ttctatgcag ttgtcgattg tggcgggtga gctcaaacgc
    19921 gcggcagatg ccgccgaaga gggcggtgat gaatttcact ggcaccgtaa tgtttatgcg
    19981 ccactgaaat attcggtagc agaaattttc gacagtatcg acctgacgca acgtctgatg
    20041 gacgaacagc agcagcaggt gaaggacgat atcgcccagt tgctgaacaa agactggcgg
    20101 gcggcgatct ccagctgtga attgttgctt tcagaaactt ccggcacgct gcgtgaattg
    20161 caggatacgc tggaagcggc aggtgataaa ttgcaggcta atctgttgcg cattcaggat
    20221 gcgacaatga ctcatgacga cctgcatttt gtcgatcgtc tggtattcga tctgcagagc
    20281 aaactcgacc gtattatcag ctggggccag caatccatcg atttgtggat tggctacgac
    20341 cgccacgtac ataaatttat tcgtaccgcg atcgatatgg ataaaaaccg cgtctttgct
    20401 cagcggttac gtcagtcggt acaaacctat tttgatgagc cgtgggcgct aacttatgcc
    20461 aatgccgatc gtctgctgga tatgcgtgac gaagagatgg tactgcgcga tgaagaagtg
    20521 actggggaac ttcctgagga tctggagtac gaagagttta acgagatccg cgaacagctg
    20581 gcggcgatca tcgaagaaca acttgccgtg tacaaaacca gacaagtgcc gctggatctt
    20641 ggtctggtgg tacgcgaata tctgtcacag tatccgcgtg cacgtcactt tgacgttgcg
    20701 cgcattgtta ttgatcaggc ggtacgtctt ggcgtagcgc aagcagattt caccggactg
    20761 ccagcgaaat ggcagccgat taatgattac ggagccaagg tacaggcgca tgtcatcgac
    20821 aaatattgaa caagtgatgc cggttaagct ggcgcaggcg ctggcgaatc cgttatttcc
    20881 ggcgctggat agcgccttac gttcaggccg ccatattggc ctcgacgaac tggataatca
    20941 tgcattcctg atggattttc aggaatatct ggaagagttt tacgcgcgtt ataacgttga
    21001 gcttattcgt gcaccggaag ggttcttcta tctacgccca cgttccacca cgctgatccc
    21061 tcgttccgtc ttgtcggaac tggatatgat ggtcgggaaa atcctctgtt atctctatct
    21121 cagcccggaa cggctggcga atgaggggat ttttactcaa caggaactgt acgacgaact
    21181 gctcaccctg gccgatgaag cgaaactgct gaaactggtg aacaaccgtt caaccggttc
    21241 agacgttgac cgtcagaagt tgcaggaaaa agtacgttct tcgctcaacc gtctgcgtcg
    21301 tttaggcatg gtgtggttta tgggccacga cagcagcaag ttccgcatta ccgaatcggt
    21361 gttccgcttc ggcgccgatg tgcgtgctgg cgacgatccc cgtgaagcac agcgtcgcct
    21421 gattcgtgat ggtgaagcaa tgccgattga aaatcatctg caactcaacg atgaaaccga
    21481 agagagtcag ccagatagcg gagaggaaga ataatgattg aacgcggtaa atttcgctca
    21541 ctgacgctga ttaactggaa cggctttttt gcccgaactt ttgaccttga cgagctggtc
    21601 acgacgcttt ctggcggtaa cggggcgggt aaatccacca ccatggcggc gttcgttacg
    21661 gcgcttatcc ccgacctgac cctgctgcat ttccgtaaca ctacggaagc cggggccacc
    21721 agcggttcgc gcgataaagg tctgcacggt aagctgaaag cgggtgtctg ttattcgatg
    21781 ctcgacacca ttaactcgcg ccaccagcgt gtagtggtcg gtgtgcgtct gcaacaggtt
    21841 gccggacgcg atcgtaaagt ggatatcaaa ccatttgcca ttcagggcct gccgatgtcg
    21901 gtgcagccga cacaactggt gaccgaaact ctgaacgaac gccaggcgcg cgtgctgccg
    21961 cttaacgagc tgaaagacaa gctcgaggcg atggaaggcg tgcagtttaa acagttcaac
    22021 tccattaccg attaccactc gctgatgttc gatctgggca tcatcgcgcg tcgtctgcgc
    22081 tcagcgtctg accgtagtaa attctatcgt ctgatcgaag cctcgctgta tggcgggatc
    22141 tccagcgcca ttactcgttc tctgcgcgac tacctgttgc cggaaaacag tggggtgcgg
    22201 aaagcgttcc aggacatgga agcggcgctg cgtgaaaacc gcatgacgct ggaagcgatt
    22261 cgtgtcaccc agtcggaccg tgacctgttt aagcatctga tcagcgaagc caccaactac
    22321 gtggcggcgg actacatgcg tcacgccaac gagcgccgtg ttcatctcga taaggccctg
    22381 gagtttcgtc gcgagctaca tacttcgcgt cagcaactgg cggctgagca gtacaaacac
    22441 gtcgatatgg cgcgagagct ggctgagcac aacggtgccg aaggcgatct ggaagcggat
    22501 tatcaggcgg ccagcgatca cctgaacctg gtgcaaaccg cactgcgtca gcaggagaag
    22561 atcgaacgct acgaagcgga tctcgatgag ctgcagatcc gtctggaaga gcaaaacgaa
    22621 gtggtggcag aagccatcga acgccaggaa gagaacgagg ctcgtgcgga agctgccgaa
    22681 ctggaagtgg acgagctgaa aagccagctt gctgactacc agcaggcgct ggacgtccag
    22741 caaacgcgcg cgatccagta taaccaggcg attgctgcgc ttaatcgtgc caaagaactg
    22801 tgccatctgc cggacttaac cgccgacagc gctgccgaat ggctggaaac cttccaggcg
    22861 aaagagctgg aagcgaccga aaaaatgctc tctcttgagc agaaaatgag catggcgcaa
    22921 accgcgcaca gccagtttga gcaggcttat cagctggtgg tggcaatcaa cggcccgctg
    22981 gcgcgtaacg aggcgtggga tgtcgctcgc gaactattgc gcgaaggggt cgatcagcgt
    23041 cacctggcag agcaggttca gccgttgcgg atgcgcttaa gcgaactgga acagcgtctg
    23101 cgtgagcagc aagaagctga gcgtctgctg gcagatttct gcaaacgtca gggcaagaat
    23161 tttgatattg atgaactgga agctctgcat caggagctgg aagcacgcat tgcctctctt
    23221 tccgatagcg tctctaacgc ccgagaagag cggatggcac tgcgccagga gcaggaacag
    23281 ctgcagtctc gcattcagag tttgatgcag cgtgcgccgg tttggctggc agcgcaaaac
    23341 agtctcaacc agttgagcga acagtgcggc gaagagttca cctccagcca ggatgtcacc
    23401 gaatttctgc aacagttgct ggagcgtgag cgcgaggcaa ttgttgaacg cgatgaagta
    23461 ggcgcgcgca aaaacgccgt cgatgaagag atcgaacgtt taagccagcc aggtggttct
    23521 gaagatcagc gtctgaacgc gctggcggag cgttttggtg gtgtgctgct gtcagaaatt
    23581 tatgacgacg ttagcctgga agatgcgccg tacttctcag cgctgtatgg cccgtcacgc
    23641 cacgccatcg tggtgccaga tctgtcacag gtaaccgaac acctggaagg cttgaccgat
    23701 tgcccggaag atctctatct gatcgaaggg gatccgcagt cattcgatga cagcgtgttc
    23761 agcgttgatg agctggaaaa agcggtggtg gtgaaaatcg ccgatcgtca gtggcgttat
    23821 tcacgtttcc cggaagtgcc gctgtttggt cgtgctgcgc gtgaaagccg tattgaaagc
    23881 ctccatgccg agcgtgaagt gctttccgaa cgcttcgcca cgctctcctt tgatgtacag
    23941 aaaacccagc gtctgcatca ggcgttcagc cgctttatcg gcagtcatct ggcggtggcg
    24001 tttgagtctg acccggaagc agaaatccgt caactgaaca gccgtcgcgt cgaactggaa
    24061 cgggcgttga gtaatcatga aaatgataac cagcagcagc gtattcagtt tgagcaggcg
    24121 aaagagggcg ttacggcgct gaaccgcatt ctgccgcgtc tcaacctgtt ggctgatgac
    24181 agcctggcgg atcgcgtcga tgaaatccgc gaacgtctgg atgaagccca ggaagccgcg
    24241 cgttttgttc agcagtttgg caatcaactg gcgaaactgg aaccgatcgt ttcggtattg
    24301 cagagcgacc cggaacagtt cgaacagtta aaagaagatt acgcgtactc tcagcagatg
    24361 cagcgcgatg cccgtcagca ggcgtttgcc ctgacggaag tggtgcagcg tcgtgcgcac
    24421 tttagctact ctgactcggc agaaatgctt agcggtaaca gcgatctcaa cgaaaaactg
    24481 cgtgaacgtc tggaacaggc ggaagcggag cgtacccgcg ctcgtgaagc gttgcgcgga
    24541 cacgcagcgc agttgaatca gtacaaccag gtgctggctt cgctgaaaag ttcgtatgac
    24601 accaaaaaag agctacttaa cgatctgcaa cgtgaattgc aggatatcgg cgtgcgtgct
    24661 gatagcgggg cagaagagcg ggcacgtatt cgccgtgacg agttgcacgc gcaactgagc
    24721 aataaccgtt cacgacgcaa tcaactggaa aaagcgctta ccttctgcga agcggagatg
    24781 gacaacctga cccgtaaact gcgcaagctg gagcgggatt actttgagat gcgcgagcag
    24841 gtagtgaccg ccaaagcggg ctggtgtgcg gtgatgcgca tggtgaaaga taatggcgtt
    24901 gagcgtcgct tacaccgtcg tgagctggct tatctctccg ctgatgattt gcgttccatg
    24961 tcggataagg cgttaggtgc gctgcgtctg gcggtggcag ataacgaaca tctgcgcgac
    25021 gtgctgcgga tgtcggaaga tccaaaacgt ccggaacgta aaattcagtt cttcgtggcg
    25081 gtttatcagc atctgcgtga acgtattcgt caggatatta ttcgtaccga tgatccggtg
    25141 gaagctatcg aacagatgga gatcgagctt agccgtctga ccgaagaatt aacctcccgt
    25201 gaacagaaac tggcgatcag ttcccgcagc gtggcgaaca tcattcgcaa aaccattcag
    25261 cgcgagcaga accgtatccg tatgctcaac caggggttgc agaacgtatc gtttggccag
    25321 gtgaacagcg tacgtctgaa cgtgaacgta cgtgaaacgc acgccatgct gctggatgtg
    25381 ctctctgaac agcacgagca gcatcaggat ctgtttaaca gcaaccgcct gaccttctca
    25441 gaagcgctgg cgaaactgta tcaacgtctt aacccgcaga ttgatatggg gcagcgcacg
    25501 ccgcagacca tcggtgaaga actgctggat taccgcaact atctggaaat ggaagttgag
    25561 gttaaccgtg gttccgatgg ctggttgcgt gcagagtctg gtgcattgtc gaccggtgag
    25621 gcgattggta ccggtatgtc gattctggtg atggtggtac aaagctggga agatgaatct
    25681 cgccgcctgc gcggtaaaga tatctctcct tgccgcctgc tgttcctcga tgaagcagcg
    25741 cgactggacg cccgttccat cgccacgctg tttgaattgt gtgaacgttt gcaaatgcag
    25801 ctcattatcg cagcgccgga aaatatcagc ccggagaaag gcaccaccta taaactggta
    25861 cgtaaagtct tccagaatac cgaacacgtt cacgtcgtcg gcctgcgagg atttgcgccg
    25921 caacttcctg aaacgcttcc aggaagtgac gaagcgcctt ctcaggcgag ttaaaattaa
    25981 ggcggcagca atgccgcctt ttctttttcc gaaaactccg tttctgcact aaaaaagtgg
    26041 cacattactg cgcgcttttc tttaaacttc tttacattag gttatgtaaa aacgtatcgg
    26101 cgtttatata ctgaagataa gcctgatgag taacaggctt gctcgtcata ctttcgtgag
    26161 tattggcgtt gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag tatgataaaa
    26221 acagggggca agggatgttg cttaatatga tgtgtggtcg tcggctgtcg gcaatcagtt
    26281 tgtgcctggc cgtaacattc gctccactgt tcaatgcgca ggccgatgag cctgaagtaa
    26341 tccctggcga cagcccggtg gctgtcagtg aacagggcga ggcactgccg caggcgcaag
    26401 ccacggcaat aatggcgggg atccagccat tgcctgaagg tgcggctgaa aaagcccgca
    26461 cgcaaatcga atctcaatta cccgcaggtt ataagccggt ttatcttaac cagcttcaat
    26521 tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct
    26581 tccagcaaca gctggcagag gtggcgattg ccggtttcca gccgcagttt aataaatggg
    26641 tagagttact gaccgatcct ggtgttaacg ggatgacacg cgacgtggtg ctctctgatg
    26701 cgatgatggg ctatctccat ttcattgcaa atattccggt caaaggcact cgctggctat
    26761 atagcagtaa accttatgcg cttgcaacgc cgccgctttc ggtgattaac caatggcagc
    26821 tggcgctgga taaaggtcaa ttgccgacgt ttgttgcagg actggcaccg cagcatccgc
    26881 aatatgcggc gatgcatgaa tcgttactgg ccttactcag tgacaccaaa ccgtggcctc
    26941 aactgaccgg taaagcaacg ttgcgcccag gacagtggag taacgacgta ccggcgttgc
    27001 gcgaaatatt gcaacgtaca ggcatgttgg acgggggacc gaaaattacg ctacctggcg
    27061 atgacacgcc aactgacgcg gtagtcagcc catccgctgt tactgttgaa acagcagaaa
    27121 ctaagctgat ggacaaacaa actacgtctc gtagtaaacc tgcgcctgcc gttcgcgccg
    27181 cctacgataa tgaactggtg gaagccgtta aacgttttca ggcatggcaa ggattgggag
    27241 cagatggtgc tattggcccg gcaacgcgtg actggttaaa cgtaacgccc gcccagcgtg
    27301 ctggcgtgtt ggcactcaac atccagcgat tgcgcttgct gccaacagag ctttctaccg
    27361 ggatcatggt taacattcca gcctattcgc tggtctacta tcagaacggc aatcaggtgc
    27421 tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg agcagtgccc
    27481 ttaacaacgt ggtggtaaac ccgccgtgga acgtcccacc aactctggca cgcaaagata
    27541 ttctgccgaa agtgcgcaat gatcctgggt atctcgaaag ccatggctat acggtgatgc
    27601 gcggctggaa cagcagagaa gcgattgacc catggcaggt tgactggtct acaatcacgg
    27661 cctcgaattt accgttccgc ttccagcagg ctccaggccc acggaactcg ctggggcgct
    27721 ataaattcaa tatgccgagt tcagaggcca tttatttgca tgacacgccg aaccacaatc
    27781 tgttcaagcg tgatacacgc gcattgagct caggctgtgt acgagtgaat aaagcttccg
    27841 atctggcgaa tatgctgttg caggatgcag gctggaatga caaacgtatt tctgatgcgc
    27901 tgaagcaggg cgatacgcgt tacgtcaata ttcggcagtc gattccggtg aatctctact
    27961 atctgacggc ctttgttggt gcagatggtc gtacccagta tcgtacagat atttacaatt
    28021 atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt
    28081 aaatgaagca gttcggggaa atcagttgtc gtaataatta gcatgatcgg gggcgattct
    28141 ctgcagcccc cgtcactgct ggggttgagt catcttgacg tctgctttac gggcggttaa
    28201 ggtgcctctt gtgcgccaga agtgcatata aacgataaca ttgacctgta gacttgatta
    28261 tcatggacaa attcgacgct aatcgccgca aactgctggc gcttggtggc gttgcactcg
    28321 gtgccgccat cctgccgacc ccggcgtttg caacactctc taccccacgc ccgcgcattt
    28381 tgacactcaa taatcttcat accggagagt caatcaaagc ggagtttttc gatggcagag
    28441 gctatattca ggaagaattg gcaaaactta accatttttt ccgcgattac cgcgcgaaca
    28501 aaataaagtc catcgaccca ggattattcg accagttgta tcgcctgcaa gggttgttag
    28561 gcacccgcaa accggtgcaa ctcatttccg gttatcgttc cattgatacc aacaatgaac
    28621 tacgcgcccg cagccgtgga gtagcgaaga aaagctatca cactaaaggc caggcgatgg
    28681 atttccacat tgaaggtatc gcgttaagca atattcgcaa agccgcgtta tctatgcgcg
    28741 caggtggtgt aggatattac ccacgtagta actttgtgca tattgatacc gggccagcac
    28801 ggcactggta gtaatcgctt aacgaaacag gggcagtatg aactatcgta ttattccggt
    28861 caccgcattc tcccagaact gttcattgat ctggtgtgaa caaacccgtc tggccgcact
    28921 ggtcgatcct ggcggcgatg cggaaaaaat caaacaggaa gttgatgcca gcggcctgac
    28981 actgatgcag atcctgctga ctcatggtca tctggaccac gttggcgcag cggcggaact
    29041 ggcgcaacat tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt tctggctgca
    29101 aggcttgcct gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc tgacgccaga
    29161 tcgttggctg aacgaaggcg ataccatcag catagggaat gtgactttac aggtgttaca
    29221 ttgccctggg catacgccgg gtcatgtcgt gtttttagat gatcgggcaa agctgctgat
    29281 ttctggcgac gttattttca aaggtggagt agggcgcagt gacttcccgc gtggcgatca
    29341 taatcaactg atttcttcaa tcaaagataa attgctgcca ctgggggatg acgtgacatt
    29401 tattccgggt cacggaccat tatccacact tggttatgag cgcctgcata atcccttcct
    29461 gcaagacgaa atgcccgtct ggtaaaaagg cgcataaaaa agcccgcttt caagcgggct
    29521 tcatcggttt taacgcttac agcactgcca caatcgcttc gcacagcggc gccatgttat
    29581 ctggtgtcat cccagccacg tttacgcgac cagaagcaac agcatatacg ccaaactctt
    29641 cgcgcagacg cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccattctgtt
    29701 tgatgataaa gctgaagtcg cgatttgcgc ctttttcctg cagcgtattg acgaacaact
    29761 gacgcatacg ctgaatacgc tggcgcatat cagtcagttc ttgttcccaa atcgcacgta
    29821 acgcatcgtt gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt
    29881 agttagcacg aatcgccgct ttcatttggc tgaatgcgcg ttcaacagtt tcactgtcag
    29941 cagcaaccag agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg
    30001 aactggcaac aatcagctcc ttatgcagag ccgcgaaagc gcgcagtcct tcagcatctt
    30061 cttccaggcc acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct
    30121 caacggagag ttgtgccagt gtctgccatt gttccagcgt agggtcgatg ccggttgggt
    30181 tatggcagca gccatggaac agcactacgt cgccagcctg ggcttcgttc aggctgttaa
    30241 tcagtgcatc gaagtcaaga gtgtgatttt ccgcatcata ataagcgtat tcacgaactt
    30301 ccagacctgc agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca
    30361 cacgcttaac gctggtattt tttgccagga aatcggcagc cacgcgtagt gcgccagtgc
    30421 cacccggagt ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa
    30481 acagcagttc ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt
    30541 tggtggtttc attttcgagc agatactgtt cagccttttt cacgctagtc agtaccgggg
    30601 ttttacccgt ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat
    30661 cggcacgaaa cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct
    30721 caaacatgac gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga
    30781 gatttacaaa attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga
    30841 ttgctctgga aggcataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa
    30901 gaggtgtgct attagaactg gtaaacgata cccacagcaa cggtgtcgtc agaacctacg
    30961 cccagtttgt tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt
    31021 ttgttgaaat agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata
    31081 ccttctacgt ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatca
    31141 aactggtatt gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt
    31201 gtaaatttat tagtgatcgg cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag
    31261 atgttgttcg cgtcatactt cagaccagta gcccactgtt cagctttttt gcctttgcca
    31321 agggaagact cttcctgcag gttggtacgg tcagcggcac cataagcacc aacgatacca
    31381 aagccttcgt attcgtagct gatagaaccg ccaacaccgt cgccgttaga gcggcgtgca
    31441 gtgtcacgct cgtttttacc caggtactga acagcgaagt tcagaccatc aaccagacca
    31501 aagaagttgg agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaaatcatcg
    31561 ctgtatgcag tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa
    31621 accacaccgt agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg
    31681 aatgccagac gcgttttgtt accagtttga gcgtcagcgc cctcagagtt gttaccctgg
    31741 aagttatatt cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta
    31801 aaaccaagac gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc
    31861 ttggagaaat aatgcagacc gacagcttta ccgtacagat ctactttgtt gccatctttg
    31921 ttatagattt ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc
    31981 agaatattgc gcttcatcat tatttattac cctcatggtt tttttatgac acctgccact
    32041 gccgtcaata agttctgtca ataaaaattt acggaactat tgatgagagt ttggtgtctt
    32101 tatgtgtctg caggcatctt tccattcaaa ctaacgtttc gctaccgtga aagtgctaca
    32161 aagataaaga tttggtttca aaaagaaaaa atatgtgaca aaaagtaaaa attaaggaac
    32221 tttgtgaac
//
LOCUS       DAZKHK010000050        31709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKHK010000050 DAZKHK010000000
VERSION     DAZKHK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.050"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..401
                     /gene="pduA"
                     /locus_tag="ACHOS5_002295"
     CDS             117..401
                     /gene="pduA"
                     /locus_tag="ACHOS5_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFP9777108.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            398..1210
                     /gene="pduB"
                     /locus_tag="ACHOS5_002296"
     CDS             398..1210
                     /gene="pduB"
                     /locus_tag="ACHOS5_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFP9777109.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1229..2893
                     /gene="pduC"
                     /locus_tag="ACHOS5_002297"
     CDS             1229..2893
                     /gene="pduC"
                     /locus_tag="ACHOS5_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFP9777110.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NSVVTELDGKPANQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNIKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDINDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2903..3571
                     /locus_tag="ACHOS5_002298"
     CDS             2903..3571
                     /locus_tag="ACHOS5_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFP9777111.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKSVSFRAPVSATVSSVPDTEN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3586..4104
                     /gene="pduE"
                     /locus_tag="ACHOS5_002299"
     CDS             3586..4104
                     /gene="pduE"
                     /locus_tag="ACHOS5_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFP9777112.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPSASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAQICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4114..5946
                     /locus_tag="ACHOS5_002300"
     CDS             4114..5946
                     /locus_tag="ACHOS5_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFP9777113.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTCPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5936..6286
                     /locus_tag="ACHOS5_002301"
     CDS             5936..6286
                     /locus_tag="ACHOS5_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFP9777114.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHARRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6306..6581
                     /gene="pduJ"
                     /locus_tag="ACHOS5_002302"
     CDS             6306..6581
                     /gene="pduJ"
                     /locus_tag="ACHOS5_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFP9777115.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6605..7027
                     /locus_tag="ACHOS5_002303"
     CDS             6605..7027
                     /locus_tag="ACHOS5_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFP9777116.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLHANKRGE"
     gene            7030..7662
                     /locus_tag="ACHOS5_002304"
     CDS             7030..7662
                     /locus_tag="ACHOS5_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFP9777117.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKLLMQPGQFAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IKGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7659..8150
                     /gene="pduM"
                     /locus_tag="ACHOS5_002305"
     CDS             7659..8150
                     /gene="pduM"
                     /locus_tag="ACHOS5_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFP9777118.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQEIVHQY
                     ASLQIRYVDLPLLRQLAGNETSDRAAIQIHEALAWGLHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8154..8429
                     /gene="pduN"
                     /locus_tag="ACHOS5_002306"
     CDS             8154..8429
                     /gene="pduN"
                     /locus_tag="ACHOS5_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFP9777119.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8441..9448
                     /gene="pduO"
                     /locus_tag="ACHOS5_002307"
     CDS             8441..9448
                     /gene="pduO"
                     /locus_tag="ACHOS5_002307"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFP9777120.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTIRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9445..10830
                     /gene="pduP"
                     /locus_tag="ACHOS5_002308"
     CDS             9445..10830
                     /gene="pduP"
                     /locus_tag="ACHOS5_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HFP9777121.1"
                     /translation="MNNSELETLIRNILREQLVPATPRMQRNAIFQTVDEAVCAAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESAKETGMGNKEDKYLKNKAALNNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKYVSLKLITTIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKVSVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVADYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSALLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10841..11953
                     /locus_tag="ACHOS5_002309"
     CDS             10841..11953
                     /locus_tag="ACHOS5_002309"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFP9777122.1"
                     /translation="MKTFSLQTRLYSGTGSLNILNRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQILRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDSEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     STRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11950..13293
                     /locus_tag="ACHOS5_002310"
     CDS             11950..13293
                     /locus_tag="ACHOS5_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9777123.1"
                     /translation="MNVELTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13296..13850
                     /gene="pduT"
                     /locus_tag="ACHOS5_002311"
     CDS             13296..13850
                     /gene="pduT"
                     /locus_tag="ACHOS5_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFP9777124.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13850..14200
                     /gene="pduU"
                     /locus_tag="ACHOS5_002312"
     CDS             13850..14200
                     /gene="pduU"
                     /locus_tag="ACHOS5_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFP9777125.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14205..14648
                     /locus_tag="ACHOS5_002313"
     CDS             14205..14648
                     /locus_tag="ACHOS5_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP9777126.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVKNNGVTELFALLHTEEGCR"
     gene            complement(14748..15077)
                     /gene="tmaR"
                     /locus_tag="ACHOS5_002314"
     CDS             complement(14748..15077)
                     /gene="tmaR"
                     /locus_tag="ACHOS5_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP9777127.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15249..16307)
                     /gene="yeeA"
                     /locus_tag="ACHOS5_002315"
     CDS             complement(15249..16307)
                     /gene="yeeA"
                     /locus_tag="ACHOS5_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9777128.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16505..16978)
                     /gene="sbmC"
                     /locus_tag="ACHOS5_002316"
     CDS             complement(16505..16978)
                     /gene="sbmC"
                     /locus_tag="ACHOS5_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP9777129.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17097..18263)
                     /gene="dacD"
                     /locus_tag="ACHOS5_002317"
     CDS             complement(17097..18263)
                     /gene="dacD"
                     /locus_tag="ACHOS5_002317"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP9777130.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            18472..19899
                     /gene="sbcB"
                     /locus_tag="ACHOS5_002318"
     CDS             18472..19899
                     /gene="sbcB"
                     /locus_tag="ACHOS5_002318"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP9777131.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSENVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19973..21331)
                     /gene="plaP"
                     /locus_tag="ACHOS5_002319"
     CDS             complement(19973..21331)
                     /gene="plaP"
                     /locus_tag="ACHOS5_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP9777132.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQIVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21321..21383)
                     /gene="yoeI"
                     /locus_tag="ACHOS5_002320"
     CDS             complement(21321..21383)
                     /gene="yoeI"
                     /locus_tag="ACHOS5_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP9777133.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21598..22527)
                     /gene="yeeY"
                     /locus_tag="ACHOS5_002321"
     CDS             complement(21598..22527)
                     /gene="yeeY"
                     /locus_tag="ACHOS5_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9777134.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22573..23397)
                     /gene="yeeZ"
                     /locus_tag="ACHOS5_002322"
     CDS             complement(22573..23397)
                     /gene="yeeZ"
                     /locus_tag="ACHOS5_002322"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9777135.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23480..23734)
                     /gene="yoeB"
                     /locus_tag="ACHOS5_002323"
     CDS             complement(23480..23734)
                     /gene="yoeB"
                     /locus_tag="ACHOS5_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP9777136.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23731..23982)
                     /gene="yefM"
                     /locus_tag="ACHOS5_002324"
     CDS             complement(23731..23982)
                     /gene="yefM"
                     /locus_tag="ACHOS5_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP9777137.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            24265..24315
                     /gene="hisL"
                     /locus_tag="ACHOS5_002325"
     CDS             24265..24315
                     /gene="hisL"
                     /locus_tag="ACHOS5_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP9777138.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    24291..24414
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            24461..25360
                     /gene="hisG"
                     /locus_tag="ACHOS5_002326"
     CDS             24461..25360
                     /gene="hisG"
                     /locus_tag="ACHOS5_002326"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP9777139.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25366..26670
                     /gene="hisD"
                     /locus_tag="ACHOS5_002327"
     CDS             25366..26670
                     /gene="hisD"
                     /locus_tag="ACHOS5_002327"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP9777140.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASENITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVVVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSTLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26667..27737
                     /gene="hisC"
                     /locus_tag="ACHOS5_002328"
     CDS             26667..27737
                     /gene="hisC"
                     /locus_tag="ACHOS5_002328"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP9777141.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            27737..28804
                     /gene="hisB"
                     /locus_tag="ACHOS5_002329"
     CDS             27737..28804
                     /gene="hisB"
                     /locus_tag="ACHOS5_002329"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP9777142.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28804..29394
                     /gene="hisH"
                     /locus_tag="ACHOS5_002330"
     CDS             28804..29394
                     /gene="hisH"
                     /locus_tag="ACHOS5_002330"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP9777143.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29394..30131
                     /gene="hisA"
                     /locus_tag="ACHOS5_002331"
     CDS             29394..30131
                     /gene="hisA"
                     /locus_tag="ACHOS5_002331"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP9777144.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVATLLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30113..30889
                     /gene="hisF"
                     /locus_tag="ACHOS5_002332"
     CDS             30113..30889
                     /gene="hisF"
                     /locus_tag="ACHOS5_002332"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP9777145.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30883..31494
                     /gene="hisIE"
                     /locus_tag="ACHOS5_002333"
     CDS             30883..31494
                     /gene="hisIE"
                     /locus_tag="ACHOS5_002333"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP9777146.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
BASE COUNT         8028 a         8233 c         8077 g         7371 t
ORIGIN      
        1 caattttatt atatttgttc tctattaatt tttctcatta gccgattaag tcagccattc
       61 tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag gtctttatgc
      121 aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata gaggccgcag
      181 acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata ggttccgggc
      241 tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca gacgcgggtg
      301 ccgcagctgc gcgtaacgtc ggcgaagtaa aagccgtaca cgttattccc cgcccacata
      361 ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg aactggtcga
      421 acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg ttctcccgaa
      481 aaccactcat caaatacgag agacggctat ggcagaaaaa agctgcacgt taacagaatt
      541 tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg atagcgccct
      601 gttggacgcg atgaaacttg aaaaacgcta tcgttccatt ggcattctgg gggcgcgtat
      661 tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca acactgaagt
      721 tattagcatt gaacttcctc gggatactaa aggcggtgcc ggacacggct cattgatcct
      781 cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagttgccc tgaaagaact
      841 cgatcggacc tttggtgatg tctatgccaa cgaagccggg catcttgaac tgcaatacac
      901 cgcccgcgcc agctatgcac tggaaaaagc gtttggcgct ccactgggac gggcttgcgg
      961 cgttattgtc ggcgctccgg ctgcggtggg tgttctgatg gccgacaccg cgctgaagtc
     1021 cgccaatgtg gatgtcgtgg catacagctc ccccgctcaa ggcaccagct acagcaacga
     1081 agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca tctccgcacg
     1141 cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg atcgcccttc
     1201 ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag cactggcaaa
     1261 acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct tcattgcgat
     1321 ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacagcgtcg ttaccgaact
     1381 ggatggaaaa cccgccaatc agtttgacct gatcgaccac tttatcgccc gctacggcat
     1441 caatctcgcg cgcgcagaag aagtcatcgc gatggattcg gtaaaacttg ccaatatgtt
     1501 gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa tgacgcctgc
     1561 aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg cgatgcagaa
     1621 aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacatca aagataaccc
     1681 ggtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg aacaggaaac
     1741 caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg tcggctcaca
     1801 ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg aactgaaact
     1861 cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta cagaacctgt
     1921 tttcaccgat ggcgatgata ccccgtggtc aaaagggttt ctcgcgtcat cctatgcttc
     1981 tcgcggctta aaaatgcgct ttacttccgg ctccggctca gaagtacaga tgggttacgc
     2041 ggaaggcaaa tcaatgcttt atctcgaggc tcgttgcatt tacatcacta aagccgcagg
     2101 cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcgg ctgtgccatc
     2161 aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc tggaatgcgc
     2221 ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac gtttcctgat
     2281 gcaatttctg ccaggtaccg actttatctc ttccggtttt tccgctgtgc cgaactacga
     2341 caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca acgtcattca
     2401 gcgtgacctg aaggttgatg gcgggctgcg accagtacgc gaagaagatg ttatcgcaat
     2461 ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac taccgccaat
     2521 taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcgaaagata tgcctgagcg
     2581 caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc gtaacagcct
     2641 ggaagtggtg aaagccctgg cgcaaggcgg ttttaccgat gtcgcccagg acatgctcaa
     2701 catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca tcgtcgatga
     2761 cggacaagtg ctctctgcgg tcaatgacat caatgattac gccggaccag ctacaggtta
     2821 ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac ttgatcccaa
     2881 cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc agattatcga
     2941 agacgtgctg gcagaaatgc aaaccagcga caagtccgtt tcatttcgcg cacctgtctc
     3001 tgcaactgtc tcttcagtgc cagataccga aaattttctg accgaaattg gcgaagccca
     3061 acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg gcctggcgca
     3121 aaccgtcaat atcatcggta ttccgcataa aaacattttg cgcgaagtga ttgccggtat
     3181 cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg atgtcgcatt
     3241 cgtagcagtc gaaggagatc gtcttagtgg ttcaggtatt gctatcggta ttcagtcgaa
     3301 aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg aacttttccc
     3361 acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg ccgcgcgtta
     3421 tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg cgcggccgaa
     3481 ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg tcactggcaa
     3541 aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa taccgatgca
     3601 attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga tgcagctcct
     3661 gtttctgcgg tccccagcgc gtcaatacag agcgccaaag tgacggatta cccgctggca
     3721 aataaacatc cggaatgggt taaaacagca accaataaaa cgctcgacga tttcactctg
     3781 gaaaatgtgc taagcgataa cgtcaccgcc caggatatgc gcattacccc tgaaacattg
     3841 cgtattcagg ccgcgattgc cagagatgct ggtcgtgatc gcctggcgat gaatttcgaa
     3901 cgcgccgctg agcttacctc agtaccggat gatcgcatcc tcgaaatcta caatgcgtta
     3961 cgcccttatc gttcgactaa gcaagaactt attgccattg ctgacgatct tgaacggcgt
     4021 tatcaggccc aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta tgtcgagcgc
     4081 aaaaaactga aaggcgacga ttaaggacga attatgcgat atatagcagg cattgatatc
     4141 ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga gttgtcgttc
     4201 gtgagtagcg ccctggcgga aaccaccgga attaaaggta cgttgcgtaa cgtccatggt
     4261 attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag cgatatttcg
     4321 cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga aaccatcact
     4381 gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc gggcggagtg
     4441 gggctgggcg taggactcac catcactccg caagagttat taacatgccc ggcggacaca
     4501 ccctacattc tggtggtgtc atcggcattt gattttgccg atatcgcaac gatgataaac
     4561 gcctccgtac gagccggata tcagctgact ggcgttattc tgcaacgcga cgatggcgta
     4621 ctggtcaata accgcctgga aattcctcta cctattgttg atgaagtcct ctacatcgat
     4681 cgtattccgc tcggcatgct ggctgcaatt gaagttgccg ttcccgggaa agttatcgaa
     4741 accctgtcta acccgtatgg gattgccacc gttttcgccc tcaatgctga agagactaaa
     4801 aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt agtgaaaacg
     4861 ccttctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact gctttctgcc
     4921 gggcgcacaa cgcgggttga tgtagctgct ggtgccgatg ccattatgaa agcggtcggt
     4981 gagtgcccaa aactggaaaa cgtgacggga gaacctggca ccaacatcgg cggaatgctg
     5041 gaacatgttc gccagactat ggctgaactg accaacaaac ccagcaacga aatttttatt
     5101 caggatttgc tggcgataga tacctctgtc cccgtcaacg taaccggtgg tctggcaggt
     5161 gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga ccgtctgcaa
     5221 atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca ggtcggaggc
     5281 gcagaagcag aagccgccat tcagggggcc ttgacgaccc ctggaacgac acgtccactg
     5341 gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca gaaaggggaa
     5401 atagttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat cgctcgtgag
     5461 ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt ggcaaaagta
     5521 gaaagcctgt tccacttacg ccatgaagat ggcagcgttc agttcttccc tactccccta
     5581 tcccctcatg tatttgcccg cgtttgtgtg gtgaaacctg acgaactggt gcctattccc
     5641 ggtgatttga cgctggaaaa agtacgcgct gtccgtcgca gtgcgaaaga gcgtgtcttc
     5701 gtaactaatg ccctgcgtgc gctgcgtcag gtaagccctg ccggaaatat tcgcgacatt
     5761 ccgttcgtcg ttctggtagg tggctcttcg ctggatttcg aagtaccgca attggtcacc
     5821 gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg tagcgaaggt
     5881 ccgcgcaatg cggtagctac tgggctcctc atcgcctggc acaaggagtc gattcatgga
     5941 aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta tcactacatg
     6001 ggatgaagtg cttcttggca ttgaagagga aggtattccc tttcgcattc aacacattcc
     6061 gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc tgttggttgg
     6121 cattgcctgc gatcaggaaa aactgattgt gcattacaaa aacttaccgg catcagcgcc
     6181 actgtttacg ttgatgtatc aacaagataa ccatgccagg cgcagcatcg gcactaacgc
     6241 tgcccgcctg gtaaaaggga ttcccttccg ggaactccac tcataatcca caggagaaac
     6301 gcagtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc gcaattgaag
     6361 ctgcagatgc catggttaaa tccgccaacg tgcaactggt tggctatgaa aagattggtt
     6421 cgggtctgat tacggtgatg gtccgtggcg atgttggcgc agtaaaagcc gccgttgatg
     6481 caggaagcgc cgccgcaagc gccgttggcg aagtgaaatc ctgccatgtc atcccgcgcc
     6541 cacacagtga tgttgaggct attttaccta aatcagcata accccattcg ctaaggagca
     6601 cagcgtgaag caatcactgg gattacttga agtcagtggt ctggcactcg ccatcagttg
     6661 cgcagatgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga agaccaatgg
     6721 ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg cggcgatcgc
     6781 gacgggagcc agtttcgccg gacagcgaaa tggcctcgtc gcacacaaag tgattgcccg
     6841 tccgggagac ggcattctgc cccacacaac tgaagcattg actgtgacgg ttgaaacaag
     6901 cgaaacacca gcaacaaccg tcatctcaga gattatcagt tgtaatttgt gtcttgaccc
     6961 ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgcacgcca ataagcgagg
     7021 ggagtaacag tggataaaca aaccttgcag gaaacagtga gccagattct ggatgagatg
     7081 cgcgagcgcc ctatccctct gggtgtttcc agtcgtcata ttcatctttc aacagcggat
     7141 tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agctgttaat gcaacctggc
     7201 caattcgctg ctgaacaggt tgttacgctg gagggaccaa aaggtaaatt gcacaacgtt
     7261 cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccgtac ggacgccaga
     7321 acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac tccagggatt
     7381 cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt cgcacaacgg
     7441 catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg cgataaagtg
     7501 gcggttgcca ttaaaggtga tgagcgacga ctgatattcg aaaacgtcgc tgttcgcgtc
     7561 tctccggata tgcgtcttga aatgcatatt gataccgatg aagccaacgc cgcaggggct
     7621 gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg ctgatgcaac
     7681 gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca ctcgtgctga
     7741 ctgtttttca actgcgagac gccagcgttc aagagatagt tcatcagtat gcatcattac
     7801 aaatccgata tgtcgatctg ccgctgctac gacaacttgc ggggaacgaa acgtcagatc
     7861 gagcagcaat tcagatacac gaagcgttag cctggggatt gcacattcaa ttgtcgttgc
     7921 agcgtcattt cctgaacgcc attgaattga agactctcgc ccgtctaccg ctaagctggt
     7981 gcgacgagca agggcaaccg atctaccttc atcgtgatcg acttttaagt tatgccgata
     8041 ttgcacagtt aagctcagga atactggtgt tacagcgaaa atgttgtgtg accgcactgg
     8101 ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa cctatgcatc
     8161 tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg atcgggaaaa
     8221 aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg ccattaaccg
     8281 gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta ctcctcagca
     8341 gtggttccag cgccagacat gtgttttccg gccctaacga agccatcgac ctggcagtgg
     8401 tcggcattgt cgatacgctt tcacgctaag gagttgaaag atggcaattt atacccgaac
     8461 aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa ctcatctacg
     8521 tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt gttattgcgc
     8581 caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga tattttggtt
     8641 tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg cagcagcgtt acatcggtac
     8701 ggaagaaatt gccgcgctgg aaaacgcgat cgatagcgca atgaacgccg tccccccggt
     8761 tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt tcgcccgcac
     8821 ggtggctcga cgggctgaaa gacgtctggt tgaactgaca acggaaacca ccatccggaa
     8881 tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg cccgcgtgga
     8941 agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt atcacgaagc
     9001 caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg atcttcacca
     9061 gctcattcgt agtgcggcaa tgcgcgcaga tgaactgcat attcccgttg tcatcagcat
     9121 tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc tgttggtcag
     9181 tagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg caacccacaa
     9241 acttgctgat accgtgcaac ccggcgcgcc gctttacggg ctcgaaagcc atatgcaagg
     9301 caaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat tacttggcgg
     9361 gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa gcgcaatggc
     9421 ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact gatccgcaat
     9481 attctgcgtg aacaacttgt ccccgcaaca ccacgaatgc aaagaaacgc tattttccag
     9541 actgttgatg aagcggtctg cgcagcacac caggcctttt tacgctttca acagtgcccg
     9601 cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc ccgccttacc
     9661 gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga taagtaccta
     9721 aagaacaaag cagcactcaa taatacgcca ggaattgaag atttaaccac cactgcacta
     9781 accggtgatg gtggcatggt tttgtttgaa tactccccgt ttggggttat tggctcggtc
     9841 acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat gctggcagca
     9901 ggtaatagcg tctacttcag tccacacccg ggagccaaat acgtttcgtt aaaactgatt
     9961 accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacctgat cgttaccgta
    10021 tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaagt cagcgttctg
    10081 gcgattaccg gcggcccggg gatcgtcgcc atgggaatga aaagtggcaa gaaagtgatt
    10141 ggtgccggag cgggtaaccc tccgtgtatc gtcgatgaaa cagcggatat tgtgaaagcc
    10201 gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat cgctgaaaaa
    10261 agcctgattg tcgtggcgtc agtggcagat tatttaattc aacagatgca aagtttcggc
    10321 gcacttcttc ttaaccatga acaaacggag aaattgcgtg caatttgcct gccagacggt
    10381 agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc agcgggcctc
    10441 cctttgcccg caaaagcgcc gcgcctactg attgctgttg ttgacgccga tgatagttgg
    10501 gttacgtgtg aacaactgat gccaatgctg cccattgtga aggtcaacga ttttgacagc
    10561 gctctcgccc tggcgttgaa agtggaagac ggcttgcacc acacggcaat tatgcactcg
    10621 cagaacgtct cacggctcaa ccttgccgcg cgcgttatgc aaacctcaat ctttgtcaaa
    10681 aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac ctttaccatt
    10741 gcaacaccga ccggagaggg tacaacgtca gcacggactt ttgcccgctc acgacgctgc
    10801 gtgttaacca gcggcttttc cattcgctag agaaacggaa atgaaaactt tttcactgca
    10861 gacgcgttta tacagcggaa cgggaagtct caacatactg aaccgtttca cgaataggca
    10921 catctggatt atttgcgacg tctttctggc acactcacca ctcattgaca cgttgcatca
    10981 ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg acccaactat
    11041 ccagaccgtg gtgaaaggca ttgcgcaaat gcagatttta cgcccggatg tggttatcgg
    11101 ttttggcggc ggttctgccc tggacgcagc aaaagcgatt gtctggttcg gtcgccagtg
    11161 tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtaccg gttccgaagt
    11221 gaccagtgct tgtgtcatca gcgattctga gaaagggatt aaatatcccc ttttcgataa
    11281 tgcactttat ccggatatcg cgatccttga tccatctttg gtcgtcagcg tgccaccggc
    11341 gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt atgtttctcc
    11401 ccgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg tttttcaata
    11461 cctgcccact gcagtgaaga aaggcgattg tctttccacc cgtggcaaaa tgcataatgc
    11521 atcgacgctt gcaggaatag cctttagcca ggccggactc ggtttaaacc atgcaattgc
    11581 tcatcaactc ggcggacagt ttcatctgcc gcacggcctg gcgaatgcgc tcctgctcac
    11641 ggcggtaatc cgctttaatg ctggcgatcc gcgcgcagcc aaacgctacg cccggctggc
    11701 gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga atgcacttat
    11761 tcaacatatt gaacagctta aaacaacctg tacgctgcca actcttgcca atgcgctgaa
    11821 agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcag cgctggcgga
    11881 tgcaacgttg cgtaccaacc cgcgtgcggc tgatgcctcc gcgattgcag aactgctcga
    11941 ggagttgtta tgaacgtcga gttaacctcc tgcgacgacc agaccatccg cgagcgagta
    12001 caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat taagctacaa
    12061 gcgcgtgttg acacgttcct ggtgaatgcg gcggaatgcg aaccaatgtt gaaagtggat
    12121 caacagttaa tggtgcagca agccagccgc ctgcttcgcg gtgttcacta tgctatgaaa
    12181 gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg ggcaataaac
    12241 gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga tgtttacccc
    12301 gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgtccc acccgcggca
    12361 ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa tattgccaga
    12421 gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ctattaatgg tgcagtggcg
    12481 aaaccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct ggcgctggcg
    12541 ggcggcgcaa cagtcgatgc gcctggtttt atcaatggcg ggccgatgat gggaagtttg
    12601 atcacatcgc tcgacacccc cgtcagcaaa accactggcg gattgctggt tttacccaac
    12661 tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc cgtcgcgaaa
    12721 actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt agtcggtcac
    12781 gaactggcac cccatttatt agtacgtgcg gttaactacc aacagctcgc tacgccacaa
    12841 ctgttactga cggcgctcac ctgttcggag tgcaacgtct gcgccagcgt cgcctgtccg
    12901 gtaggaattt caccaatgcg cataaaccgc ctgcttaagc aggaattacg cgcacaaaat
    12961 ctccgctacg aaggtgcctt aaatcctgct gatcccatgg caaattatcg actgattccc
    13021 gtaaaacggc tggtaaccag actgggccta atcccgtggt atcaggatgc gccattgtct
    13081 gaacaggtgc ctcagcccga aaaagtgacg ttattgttac gccagcacat cggtgccagc
    13141 gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg ggcaatgtgt aggtcaaatt
    13201 ccacacggca cattaggtgc cccgattcat gccagtattg atggcatggt cagcgatgtg
    13261 acagaaaacg cgattacgct tgtgagaggt taacgatgtc gaaagcggta ggtattttag
    13321 aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc catgctgaaa agcgcaaacg
    13381 ttgagttact ggtcagcaaa acgctatgtc ccggaaaatt tctgctgatg ctcggtggcg
    13441 atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc ccttgctggt gaaatgctgg
    13501 tcgacagtct tgtcctgccg aatattcatc ccagtctcct ttctgcaatc agcggactta
    13561 accatgttga acagcaccgg gctgtcggcg tagtcgaaac ctggagtgtc gccgcctgca
    13621 ttgacgctgc cgattgtgct gtcaaagctg cgaatgtcat tctcgttcgc gtacatatgg
    13681 cttttggcat tggtggtaaa tgctacatgg tggttgctgg cgatatttca gatgttaata
    13741 acgcggtctc tgtcgccagc gagcgtgcgg gggaaaaagg tttgttggtc tatcggtcgg
    13801 tgattgcccg tccacatgaa gccatgtggc gtcagatggt ggagggataa tgacaactca
    13861 gcacacaact gaacggatga ttcaggaata tgtccctggt aagcaggtca cgctggcaca
    13921 tgtcatcgcc aatcctggga aggatctgtt taagaaattg ggtttaccag acaccgtttc
    13981 tgctattggc attctgacga ttacccccag cgaagcatct atcatcgcat gcgatatcgc
    14041 cacaaaatca ggcgctgtag agatcggttt tcttgatcgt tttaccggtg ccgtagtgct
    14101 gactggtgat gtttctgcta ttgaatatgc acttagccag gttacccgta cgctgggtga
    14161 aatgatgcgc tttaccgcct gccccattac gcggacctga cgttatgcaa cgtatgatgc
    14221 ttatcggccc cagccagtgc ggaaaaacgt cactcacgca atgtctgcgt ggcgaaaata
    14281 tccattatca gaaaacgcag gcaatcgtct ggtccccgac caccattgat acaccaggcg
    14341 aatacctcga aaaccgcagc ctctacagtg cattgttggt cagtgcctgc gaagcagaca
    14401 ttatcgcgct ggtacttaat gcggacgcac cgtggtcacc tttttcgcca ggctttacct
    14461 cgtcaatgaa tcgcccggtc attggagtag taactaaagc agatttagcc agtatggaac
    14521 aaatatcttt ggtgaaatgt tggttacggg aagctggagc gcacaacgtg ttagtaacca
    14581 gtgcggttaa aaacaatgga gttacagaac tttttgcctt gctgcataca gaagaaggct
    14641 gtcgctaatt tcgataggtt ctcagatgag aatgatgacg gtgataacag ataagaatca
    14701 atgagcaatg agggttgccg ggcaaccctc attgaataaa acaggaatta cttcgcttcg
    14761 ccgtttttca tttcgcccat agctttcagc tttttggaga tatcgcggcg ttctttggag
    14821 agctcggcat ttttgatgat gtaatcgtca acgcgatctt catagtcacc tttcatgctg
    14881 gcgatgatgc cctggattgc ttcaacgctc atccctggct tgatgtaatc gctcaggttg
    14941 tccagcaaca ggacgcgctt ctggttgtca cggatctttt tctcaacgtc ctgaatttca
    15001 cgttgcagtt tgttcttacg acggaacaga cgaacaaatt ccagtacgtc ctggaatgaa
    15061 ggcttggtag tttccatttt tatacccctg ataatgtgag agtcggattc gtttaagcaa
    15121 ccgctattcg ttagggccaa cattactcat agctgccgca gatgacaatg cttttatccc
    15181 ttttcactat catacccttt atccttgctg aatcgaagca gcagcaaggt gattctgaag
    15241 ttcaggaatt atttgcgcaa ggcccggcaa atcagattcg atagcaactc aagctgatgt
    15301 gccgtttcca tgttcagcca cacataacca tagattggtg tttccacgac tttcaggtca
    15361 tggtggttat cgagcaactg acgcagctct tccacagcat cgttcaattt ttccgtattg
    15421 gcaaaaaccg gctgcggatt accttcgtac agcgcatgaa caaggctcag cagtatttgc
    15481 tgcatcatgt gctgggtatc acgaagtttt tgcgcgttca ataacacgaa atggctgggg
    15541 cgcgtggccc agtatgcatt gatttgcaac tccagcatac aaaccagatt gcgattaatg
    15601 gtctggatac cttcatatat cgatttggga atacgggttt ctttgctggc gggcgcaatc
    15661 agcccacgca ttttcacggc atcggtcagg agtttttgta gatggctttc cagacgtggg
    15721 cgttcgagta agttcggtga gaatgcagat tgatagaccc gattatactc ggtcagactt
    15781 ttcgccagtt gaatgcgcca gtggatgaat gcccgttgtg gccagatacc ggtaaacaac
    15841 attgccagta aagagccgag gatcacatcg ccgcttcgcc ataacgccgt atcaatttca
    15901 cctgtcgggg aacccacaac aattgccagc gtcaccccaa tcaataatcc ttgatacggt
    15961 ttcttgccca gcgccagcca accgcaaaga aacatcgccg ccgcgcacca gactaacatc
    16021 agcggtaacg aaattaactc cagttgcaga gcgataagac ctaaaatcga ccccaacacc
    16081 gtaccgccaa tacgttcgaa ggcacgaggg acaacgttcc cccagaacga gattggcccc
    16141 ataatcacca ccatggtgac cagcggccag gtgctttccg ggatagtaaa caggcggata
    16201 atgagaaaag tgagcaggaa tgccagcgcg acccgagtac catgcacaat gcggtaatgg
    16261 cggtataccc ggatttcaaa cgggcttaat gacttatcgg cacgcacgca gaaactccgt
    16321 aatgaagatt aaaagcgctc ataacacatc tgtcggccag aaggcgcgcc tctgagttat
    16381 ccgacggaca taccgggaac agtgtaattt cccggcagga gcgaaaaatt tgaaaggcgc
    16441 atcttactct tttcgctttc aaaaaaagag tggtcttcgc gttaacacac cgccctgaga
    16501 tgaattagtg atgttttggc tgcaccgcaa catacatttc gatatcccag tacccatctt
    16561 ccgcgccatt gttcaagtaa acctcgaagc agggctttgg taacatttca taagcactgt
    16621 cctgcaagag gctattaaag aactgatacc agggtttagc aaaatcatca ccgactacac
    16681 gagccaccgc caccgcatac tgaccacctg aaatttctgt cagaatgacg ccctcactgt
    16741 tttcggggag cgtaaagtta ttcggtaccg tcacgacggt gtcgcagcgt aatttttcgg
    16801 cgggtgtttc atctggattg tcgtaataga cagcaaccca ctccttcggc acaatatttt
    16861 tgctatctat ccacatcatc aactgctcaa aacctttctt taccgtctgc tcccacgggc
    16921 caacaagatg gaaacctgca acggtacgtt tatcttcctg cttaatctcg tagttcatga
    16981 cgcctccact aacactgttt ttatatacag tatagttgca atattaaaac cacaaggaat
    17041 gagtgttgat tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag
    17101 gccttatggt ggaaataatc actcaggcgg gaaaacatgc tgccttcccc gacagattcc
    17161 agggtaacca gcggccagtg cgccacctgt ttatcacggt cgtaaagttc aatttcccct
    17221 acccgctgat gggcgctaat tggcgcggtg agttctttac catcaagggt atatttggct
    17281 ttgatatgtg gaatttcggc tttcggtagc accatccaga actcttgttc cgttcccagg
    17341 gcgatatttt ctttatcacc ataccagatg cgttccgtac cgaccttttt cccacggtgc
    17401 aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc
    17461 tcacgacctt ttgcgctgtc agcacccatt accactgcaa tgaggcgacg ctgcccgtct
    17521 acagccgaag caataagatt aaacccggca ccagaagtat ggcccgtttt caggccgtca
    17581 acattcatgg ttttatccca caataacccg ttacggtttt gctgggtgat accgttccag
    17641 gtgaggcttt tctcactgta catatgataa aactcgggct cgccgtggat gatagcgcga
    17701 gaaagcacag ctaaatcata agccgagcta tgctggccag gtgcatccag accatgcact
    17761 gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca
    17821 aactgccgtt gcccaccggc aatatagtca gccagtgcaa cacaagcgtc atttccggaa
    17881 tccacaatta aaccacggct taaatcacgt accgatacgc gatcgccctc tttcaaaaac
    17941 atcagtgaag aaccgacaaa caccggatta tctttcgccc acgcatcacg ccccacggtg
    18001 acaatatcgt ccggcgtaat gcgatgacta tcgatagcgc gatccacgac atagcccgtc
    18061 atcagctttg tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgagaatc
    18121 tgaccggtgg tgtaatccat caatacccag gacccggcat gaatctctgg aggttgaggt
    18181 gaaaaaggaa tgttttccgc cgcaaaacca gacgataaat taaaaacgaa caaagaagca
    18241 gcaataataa gacggcgttt caacagcaaa ccctcaggtg tttcaaatag ctgttctttt
    18301 tacggaaata cttatgaact ggctggaata aagtgcaaga aaatgtgact actatctcat
    18361 ttttatctga catgatctgt tgccactcgc tgccaaattg tcgcgctaaa gctgattagc
    18421 acggtgatat ttgatactct ggcagacagc agaaataacg gatttaacct aatgatgaat
    18481 gacggtaagc aacaatctac ctttttgttt cacgattacg aaacctttgg aacgcacccc
    18541 gcgttagatc gccctgcaca gttcgcagcc attcgcactg ataacgaatt caatgtcatc
    18601 ggcgaacccg aagtctttta ctgcaagccc gcggatgact atttacccca gcctggagca
    18661 gtattaatta ccggtattac cccgcaggag gcacgggcga aaggagaaaa cgaagccgcg
    18721 tttgccgccc gtattcactc gctttttacc gtaccgaaga cctgtattct gggctacaac
    18781 aatgtgcgtt tcgacgacga agtcacacgc aacgtttttt atcgtaattt ctacgatcct
    18841 tacgcctgga gctggcagca tgataactcg cgctgggatt tactggatgt tatgcgtgcc
    18901 tgttatgccc tgcgcccgga aggaataaac tggcctgaaa atgatgacgg tctaccgagc
    18961 tttcgccttg agcatttaac caaagcgaat ggtattgaac atagcaacgc ccacgatgcg
    19021 atggctgatg tgtacgccac tattgcgatg gcgaaactgg taaaaacgcg tcagccacgc
    19081 ctgtttgatt atctctttac ccatcgtaat aaacacaaac tgatggcgtt gattgatgtt
    19141 ccgcagatga aacccctggt tcacgtttcc ggaatgtttg gggcatggcg cggcaatacc
    19201 agctgggtgg caccgctggc gtggcatcct gaaaatcgca atgccgtaat tatggtggat
    19261 ttggcaggag acatttcgcc attactggag ctggatagcg acacattgcg cgagcgttta
    19321 tataccgcaa aagccgatct tggcgataac gccgccgttc cggttaagct ggtgcatatc
    19381 aataaatgtc cggtactggc ccaggcgaat acgctacgcc cggaagatgc cgaccgactg
    19441 ggaattaatc gtcagcattg cctcgataac ctaaaaattc tgcgtgaaaa tccgcaagtg
    19501 cgtgaaaaag tggtggcgat attcgcggaa gccgaaccgt ttacgccttc agagaacgtg
    19561 gatgcacagc tttataatgg ctttttcagt gacgccgatc gtgcagcaat gaaaattgtg
    19621 ctggaaaccg agccgcgtaa tttaccggca ctggatatca cttttgttga taaacgaatt
    19681 gaaaaactgt tgttcaatta tcgagcgcgc aacttcccgg ggacgctgga ttatgccgag
    19741 cagcaacgct ggctggagca ccgccgccag gtcttcacgc cagagttttt gcagggctac
    19801 gctgatgaat tgcagatgct ggcacaacag tatgccgatg acaaagagaa agtggcgctg
    19861 ttaaaagcgc tttggcagta cgcggaagag atcgtctgaa acaaaaaagc cggagcgaca
    19921 aactccggct ttttgagatt taccggatga ttatgtcatc cggtttgtaa gattacgcta
    19981 cgtcttcgta ctgcggaacc gggttacgga aactcttggt tacgcaagcc aggtaaatca
    20041 gaccgatagc agcccagatc agacccagaa ccattgagct ttcttccagg ttaacccaca
    20101 gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag atactggaag tgatctttca
    20161 gcgttttgtt acgcttctca cggatccaga actgcgagat gaccgacagg ttaacgaagg
    20221 tgaacgccac caacgcacca aagttaatca gcgccgtagc cattaccagg tcgaagttga
    20281 ttgccagcag ggcaatcgcg ccaaccagga tgatattcat cgccggagta cgccatttcg
    20341 ggtgtacata accgaagaag cttttcggga acacgccgtc acgacccatt acgtacatca
    20401 gacgcgcaac gcctgcgtgc gccgccatac cggacgccag tacggtaatg gtggagaaga
    20461 tcagcgcgcc aacctggaac gctttacccg caacgtacag catgatttca ggctgtgacg
    20521 catccggatc tttaaaacga gagatatccg ggaagtacag ctgcaggaag taagttgcaa
    20581 agatgaagat catgccgccg atcagcgcgg tcaggaaaat cgcacgcggg atcacgcgct
    20641 ctgcatcttt ggtttcttcc gacaggttgc tgatgccgtc aaaaccggta aaggagaagc
    20701 acaggattgt cgccccggta atcatcggga taacatgcgc atcgccagac cagaacggac
    20761 gagtgctcgc cagcgtacca gcgccttcac cttcaaatac gccataaacc accatgccca
    20821 gaatcaccgc gatcagcact atctgcaaca cgacgattac agtgttgaag ttcgctacgg
    20881 atttcagact acgcaggtta aaggcggtca taaaggccac cagcgccacc acaaacatcc
    20941 acgatggaat ggaaggcacc agagcttcaa aatagatttt cgccagcaga atgttgatca
    21001 tcggcgcgaa cagatagtcg agcagagaag accaacccac cataaagccg acagtcggac
    21061 taatggattt ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg cgaaccagct
    21121 tcccgtagct cagagccgta aacaggatcg caatcaatgc gaacgcatag gctgtcggca
    21181 catgaccatc cgtgaggcct gaaacgatac caaatgtatc aaacagcgtc atcggctgca
    21241 tataggctag acccatcatt acaaccggaa ctaacgtaag cgttttacgc aattccacgc
    21301 gagaggtgtt tggagtaacg ttatgcgaca tggtcattct cctttacggt gataaccgtc
    21361 gcgtaagcaa aaaattgccc catttttttg gattcctcag cgacaacaac tgtcgatttt
    21421 tagtaaatat ctatccggta cgaagcccgg cctcttggta tgaatgattg gtttgaagca
    21481 aaaaataacc gacgctaatt aaacgtcggt ttttagtcat tttttgacag cggcgcattg
    21541 tgcctaaatg ggggggaaat gacaagagaa tgagaggctt gtcataataa tttttcttta
    21601 aatggctgat tttccgtcat cagattttgc ataaacaccg gcgatcgcac tatttgctaa
    21661 aatttcatct cgccaccagc cgctggcaag ccctgcggtc tgttcattcc agccaatcca
    21721 gacgggttca aagagagttt gggcgaccac tttcttctcg attaacgcgc cactatcgag
    21781 aaaacgttgc gccagatacc gtggtaaata gccgcagcca aggccgctaa tttgcagctc
    21841 cagcttggtt ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa gttgtgaggc
    21901 cgtagaagcg cctgcctgcg cagtgtcccc caccacaatc gcccgatatc gtttaatgat
    21961 acgacggttt aacggttctt cttcctgggc taacggatga tgaggcgcga ccgcaaagac
    22021 ttgttcaaga tcgcccaacc gcgagaaacc aaactcactg gaagatggtg gctcatgcat
    22081 cgcgccaacg ataatatccg ctcgcccctg agtcagggca tcccaggaac caccgagtac
    22141 gccattgata aacttcaggc gcgtcacgct atgatgttga taaaaagctt caatgagagg
    22201 cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc accagctcgt tttcccagcc
    22261 ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa cttcccgccc
    22321 tttctctaat aacatttttc cggtacgggt gaatttagcg cggtgtccgc tacgatcaag
    22381 caattgaata ttaaggtcgc tttccagctt gtgaacggtg taactcagag cggacggcgt
    22441 cttgtagagt ttggctgacg ccgccgcaaa actgccttct ttttctaagg catcgaggat
    22501 catcaacaca tccagcagtg gtttcataca cgcccccttg cagtgcatgc ggcgtcctgc
    22561 tggcgtgatc gtttactcca gcggcattac cagcggatcg ggatactggt attcaaatcc
    22621 cagttcatta caaatccgac tgccatcaat aatcttacct ttgccgctgt ccagactatt
    22681 tctgaactgc ggaggttcca gccccagtaa acgggccatc tgcggataga aaacattacg
    22741 cgcagggtga gctggcgcac atatattata gatgtgtccg cctttaggtg cctgtaacaa
    22801 cagagtgata gcgccaataa catcttctaa atggactaaa ttaacaccat gttcaccatc
    22861 aggggcggtt tttccggcaa agaagcgtcc gggatgacgt cccggtccga ccaggcccgc
    22921 aagacgcaga atatcgaccg aagtaccggg taaattgtgc agccagtctt cgagttcttc
    22981 taatactcgt ccactgttgg tcaccggatt acgcggggta gtttctttca ccgtgccttg
    23041 cgcgtcgcca tagacagatg ttgagctggt aaaaataatt cggggaatac gatgggccag
    23101 cgcgctatcc actaactctt gtaccgcttg taaatagaac tcatcgccgg ggccgctacg
    23161 acgtgccgga agcgtaatga ccagcgcatc ggcatccatc agggcatcca gatcgtcaga
    23221 atcgcaaact aactcaggct ccatgcgaag cagatagcta tcaatgccac tcattcgggc
    23281 cgcttcgaca ccatcttgtg tggttttact cccggtgact tgccagcctc gcgctgaaag
    23341 tgacatcgcc agcggcatac ctaaccaccc taacccgaca attgcgacct ttttcatccg
    23401 ttatctcctg atacacctta gatctataag gctacgctag cgtatcaaaa ctgacaattc
    23461 attctatgaa tgaatctgtt caataatgat aacgacatgc tgcaatgagc agtgaatcat
    23521 cagtaaccgc gtataccaga cggtgctcct ctgtaatgcg tcgggaccag aaacctgaca
    23581 aattatgttt caggggttct ggtttcccct taccttcaaa tggtgttctg cgtgtatctt
    23641 tgataagttc attgatcttt ttaacaattc gcttatctgt ttcctgccag tacagataat
    23701 catcccatga ttcctcagac cagattagtt tcactcaata atgtcctttt ccgttccttt
    23761 gcctgatttc aggctatcga ttgagtccat caatctccgg gcgttagcgg gggaacgcag
    23821 tagataagcc gtctcttcca gcgagttgta ttcttcgagt gacatcagaa cacaagcctc
    23881 tccattctga cgagtaatga ggatcggggc atgatcttca acggctttca tcattgttgc
    23941 cgacaaattc tgacgcgctt cgctgtagct aattgtacgc atgtaaatct cctcttttgt
    24001 acagttcatt gtacaatgat gggtgttaat taactattta ttaattagtt tgtagatcaa
    24061 gacattgtca gcgagacgaa aaatcagact ttgctctttt tggtgctgtc aagttagagg
    24121 acagtcctct tagccccctc ctttccccgc tcattcatta aacaaatcca tcgccataaa
    24181 atatataaaa aaccccttgc tttctaacgt gaaagtggtt taggttaaaa gacatcagtt
    24241 gaataaacat tcacagagac ttttatgaca cgcgttcaat ttaaacacca ccatcatcac
    24301 catcatcctg actagtcttt caggcgatgt gtgctggaag acattcagat cttccagtgg
    24361 tgcatgaacg catgagaaag cccccggaag atcaccttcc gggggctttt ttattgcgcg
    24421 attgataacg gttcagacag gtttaaagag gaataagaaa atgacagaca acactcgttt
    24481 acgcatagct atgcagaaat ccggccgttt aagtgatgac tcacgcgaat tactggcgcg
    24541 ctgcggcatt aaaattaatc ttcacaccca gcgcctgatc gcgatggcag aaaacatgcc
    24601 gattgatatt ctgcgcgtgc gtgacgatga cattcccggt ctggtaatgg acggtgtggt
    24661 agaccttggg attatcggcg aaaacgtgct ggaagaagag ttgcttaacc gccgcgccca
    24721 gggtgaagat ccacgctact ttaccctgcg tcgactggat ttcggcggct gccgcctttc
    24781 gctggcaacg ccggttgatg aagcctggga cggcccgctc tccttaaacg gtaaacgtat
    24841 cgccacctct tatcctcacc tgctcaagcg ttatctcgac cagaaaggta tctcttttaa
    24901 atcctgctta ctgaacggtt ctgtcgaagt tgcgccgcgc gccggactgg cggatgcgat
    24961 ttgcgatctg gtttccaccg gtgccacgct ggaagctaac ggcctgcgcg aagtcgaagt
    25021 tatctaccgc tcgaaagcct gcctgatcca gcgcgatggc gaaatggaag aatccaaaca
    25081 gcaactgatc gacaaactgc tgacccgtat tcagggtgtt atccaggcgc gcgaatcaaa
    25141 atacatcatg atgcacgcac cgaccgaacg tctggatgaa gtcatcgccc tgctgccagg
    25201 tgccgaacgc ccaaccattc tgccgctggc gggtgatcaa cagcgcgtag cgatgcacat
    25261 ggtgagtagc gaaaccctgt tctgggaaac gatggaaaaa ctgaaagcgc tgggtgccag
    25321 ttcaatcctg gtcctgccga ttgagaagat gatggagtga tcgctatgag ctttaacaca
    25381 atcattgact ggaatagctg tactgcggag caacaacgcc agctgttaat gcgcccggcg
    25441 atctccgctt ctgaaaacat tacccgcacc gttaacgata ttctcgataa cgtgaaaacg
    25501 cgtggcgatg aggccctgcg tgaatacagc gcgaagtttg ataaaaccac ggttaccgcg
    25561 ctgaaggtgt ctgctgagga gatcgccgcc gccagcgaac gcctgagcga cgagctaaaa
    25621 caggcgatgg cggtggcagt aaagaatatt gaaaccttcc acactgcgca aaaactgccg
    25681 ccggtagatg tagaaacgca gccaggcgtg cgttgccagc aggtcacgcg cccggtggct
    25741 tcagtgggtt tgtatattcc tggcggctcc gccccgctct tctcaacggt attaatgctg
    25801 gcaactccgg cgcgtattgc aggttgcaat aaagtggtgc tgtgctcacc gccgccgatt
    25861 gccgatgaga tcctttacgc ggcgcagctg tgcggcgtgc aggacgtgtt taacgtcggc
    25921 ggcgcacagg ccattgccgc gctggcgttt ggtacggaat ctgtgccgaa agtggacaaa
    25981 atcttcgggc cgggtaacgc ctttgtcacc gaagcaaaac gccaggtaag ccagcgtctg
    26041 gacggtgcgg cgatcgatat gcccgcaggt ccgtcggaag tggtggtgat tgctgacagc
    26101 ggcgctacgc cggatttcgt ggcttctgat ttgctttctc aggctgaaca cggcccggac
    26161 tcacaggtga ttttactgac ccccgacgcc gatatggcgc gtcgcgttgc cgaggccgtc
    26221 gaacgccaac tggcagaact gccgcgtgcc gaaaccgccc gccaggcact gaacgccagc
    26281 cgcctgatcg tgactaaaga tttagcgcag tgcgtggaga tctccaacca gtacggcccg
    26341 gagcacctga tcattcagac ccgcaacgcc cgcgaactgg tcgatggcat caccagcgcc
    26401 ggttcggtat ttcttggtga ctggtcaccg gaatccgcag gtgattacgc ctccggcacc
    26461 aaccacgttc taccgactta cggctacacc gccacctgtt ccagcctcgg gctggcagat
    26521 ttccagaagc gcatgaccgt acaggaactg tcgaaagaag gcttttccac gctggcttcg
    26581 accattgaaa cactggccgc cgccgagcgc ctgaccgccc acaaaaatgc cgttactttg
    26641 cgtgttaacg cccttaagga gcaagcatga gcaccgtgac tattaccgat ttagcgcgtg
    26701 aaaacgtccg caacctgacg ccgtatcagt cagcgcgtcg tctgggcggt aacggcgatg
    26761 tctggctgaa cgccaacgaa tatcccactg ccgtggagtt tcagctcact cagcaaacgc
    26821 tcaaccgcta tccggaatgc caaccgaaag tggtgattga aaattacgcg caatatgcag
    26881 gcgtaaaacc ggaacaggtg ctggtcagcc gtggcgcgga cgaaggcatt gaactgctga
    26941 ttcgcgcttt ttgcgaaccg ggtaaagacg ccatcctcta ctgcccgcca acgtacggca
    27001 tgtacagcgt cagcgctgaa actattggcg tcgagtgccg cacagtgccg acgctggaca
    27061 actggcaact ggacttacag ggcatttccg acaagctgga tggcgtaaaa gtggtctatg
    27121 tttgcagccc caacaacccc accgggcaac tgatcaaccc gcaggatttt cgcaccctgc
    27181 tggagttaac ccgcggtaag gcgattgtgg ttgccgatga agcctatatc gagttttgcc
    27241 cgcaggcatc gctggctggc tggctggcgg aatatccgca cctggctatt ttgcgcacac
    27301 tgtcgaaagc ttttgctctg gcggggcttc gttgcggatt tacgctggca aacgaagaag
    27361 tcatcaacct gctgatgaaa gtgatcgccc cctacccgct ctcgacgccg gttgccgaca
    27421 ttgccgcaca ggcgttaagc ccgcagggaa tcgtcgccat gcgcgaacgg gtggcgcaaa
    27481 ttattacaga acgcgaatac ctgattgccg cactgaaaga gatcccctgc gtggagcagg
    27541 ttttcgactc tgaaaccaac tacattctgg cgcgctttaa agcctccagt gcggtgttta
    27601 aatctttgtg ggatcagggc attatcttac gtgatcagaa taaacaaccc tctttaagcg
    27661 gctgcctgcg aattaccgtc ggaacccgtg aagaaagcca gcgcgtcatt gacgccttac
    27721 gtgcggagca agtttgatga gtcagaagta tctttttatc gatcgcgatg gaaccctgat
    27781 tagcgaaccg ccgagtgatt ttcaggtgga ccgttttgac aaactcgcct ttgaacctgg
    27841 cgtgatcccg gagctgctga agctgcaaaa agcgggatat aagctggtga tgatcactaa
    27901 tcaggatggt cttggaacac aaagtttccc gcaggcggat ttcgatggcc cgcacaacct
    27961 gatgatgcag atcttcacct cgcaaggcgt gcagtttgat gaagtgctga tttgcccaca
    28021 cctgcccgcc gatgagtgcg actgccgtaa gccgaaagta aaactggtgg agcgttatct
    28081 ggcagagcaa gcgatggatc gcgccaacag ttatgtgatt ggcgatcgcg cgaccgacat
    28141 tcaactggcg gaaaacatgg gcattaatgg tttacgctac gaccgtgaaa ccctgaactg
    28201 gccgatgatt ggcgagcaac tcacgaaacg cgatcgttat gcccacgtgg tgcgcaacac
    28261 caaagagacg cagattgacg ttcaggtgtg gctggatcgc gagggtggca gcaagattaa
    28321 taccggcgtt ggcttctttg atcacatgct ggatcagatt gccactcacg gcggtttccg
    28381 tatggaaatc aacgttaaag gcgacctcta tatcgacgat caccacactg tcgaagatac
    28441 cggtctggcg ctgggcgaag cgttaaaaat agcacttggc gacaaacgcg gtatttgccg
    28501 ctttggtttt gtactgccga tggacgaatg ccttgcccgc tgcgcgctgg atatctctgg
    28561 tcgcccgcac ctggaatata aggccgagtt tacctaccag cgcgtgggcg atctcagcac
    28621 cgaaatgatc gagcacttct tccgttcgct ctcatacact atgggcgtga ccctgcatct
    28681 gaaaaccaaa ggtaaaaacg atcaccaccg agtagagagc ctgttcaaag cttttggtcg
    28741 caccctgcgc caggccatcc gcgtggaagg cgataccctg ccctcgtcta aaggagtgct
    28801 gtaatgaacg tggtgatcct tgataccggc tgcgccaatt tgaactcggt gaagtctgcc
    28861 attgcgcgtc acggttatga acccaaagtt agccgtgacc cggacgtcgt gttgctggcc
    28921 gataaactgt ttttacccgg cgttggcacc gcgcaagcgg cgatggacca ggtgcgtgag
    28981 cgcgagttgt ttgatctcat caaagcctgt acccaaccgg tgctgggcat ctgcttaggg
    29041 atgcaactgc tggggcgacg cagcgaagag agcaacggcg tcgatttgct gggtatcatc
    29101 gacgaagacg taccgaaaat gaccgacttt ggtctgccac tgccgcatat gggctggaac
    29161 cgcgtttacc cgcaggcagg caaccgcctg tttcagggga ttgaagacgg tgcgtacttt
    29221 tactttgttc acagctacgc aatgccggtc aatccgtgga ccatcgccca gtgtaactac
    29281 ggcgaaccgt tcaccgcggc ggtacaaaaa gataacttct acggcgtgca gttccacccg
    29341 gagcgttctg gtgccgctgg cgctaagttg ctgaaaaact tcctggagat gtgatgatta
    29401 ttccggcatt agatttaatc gacggcaccg tggtgcgtct ccatcagggc gattacggca
    29461 aacaacgcga ttacggtaac gacccgctgc cacgcttaca ggattacgct gcgcaaggtg
    29521 ccgaagtgtt gcacctggtg gatctgaccg gggcaaaaga tccggctaag cgtcaaatcc
    29581 cgctgattaa aaccctggtc gcgggcgtta acgtcccggt gcaggttggc ggcggtgtgc
    29641 gtagtgaaga agacgtagcg acgttactgg aagcgggcgt tgcacgcgtg gtggttggct
    29701 ccaccgcggt gaaatcacca gaaatggtga aaggctggtt tgaacgtttt ggtgctgatg
    29761 ccttagtact ggcgctggat gtccgtattg acgagcaagg caacaagcag gtggcggtca
    29821 gcggctggca agagaactcg ggcgtttcac tggaacaact ggtggaaacc tatcttcccg
    29881 tcggcctgaa acatgtgcta tgtaccgata tctcgcgcga cggcacgctg gcaggttcta
    29941 acgtctcttt atatgaagaa gtgtgcgcca gatacccgca ggtggcattt cagtcctccg
    30001 gcggcattgg cgacattaac gatgttgctg ccctgcgagg tactggcgtg cgcggcgtaa
    30061 tagttggtcg ggcattactg gaaggtaaat tcaccgtgaa ggaggccatc gcatgctggc
    30121 aaaacgcata atcccttgtc tcgacgttcg tgatggtcag gtggtgaaag gcgtacagtt
    30181 tcgcaaccat gaaatcattg gcgatatcgt gccactggca aaacgctacg ctgaagaagg
    30241 tgcagacgaa ctggtgttct acgatatcac cgcttccagc gatggccgtg tggtagataa
    30301 aagctgggta tctcgcgtgg cggaagtcat cgacattcca ttctgcgtgg cgggcgggat
    30361 taaatcgctg gatgatgccg cgaaaattct ttcctttggc gcggataaaa tttccatcaa
    30421 ctctcctgcg ctggcggacc cgacgttaat tactcgcctg gcggatcgct ttggtgtgca
    30481 gtgtattgtg gtcggtattg atacctggta cgacgccgaa accggtaaat atcatgtgaa
    30541 tcaatatacc ggcgatgaaa gccgcacccg cgtcactcag tgggaaacac tcgactgggt
    30601 acaggaagtg caaaaacgtg gagccggaga aatcgtcctc aacatgatga atcaggacgg
    30661 cgtgcgtaac ggttacgacc tcgaacagct gaaaaaagtg cgtgaagttt gccacgtccc
    30721 gctgattgcc tccggtggcg caggcaccat ggaacacttt ctcgaagcct tccgcgatgc
    30781 cgacgttgac ggcgcgctgg cagcttccgt attccacaaa caaataataa atattggtga
    30841 attaaaagcg tacctggcaa cacagggcgt ggagatcagg atatgttaac agaacaacaa
    30901 cgtcgcgaac tggactggga aaaaaccgac ggacttatgc cggtgattgt gcaacacgcg
    30961 gtatccggcg aagtgctaat gctgggctat atgaacccgg aagctttaga caaaaccatc
    31021 gaaagcggca aagtcacctt cttctcgcgc actaaacaac ggctgtggac caaaggcgaa
    31081 acgtcgggca atttcctcaa cgtagtaaat attgcgccgg actgcgacaa cgacacgcta
    31141 ctggtactgg cgaatcctat cggaccaacc tgccacaaag gcaccagcag ctgcttcggc
    31201 gataccgctc accagtggct gttcctgtat caactggaac aactgctcgc ggaacgtaaa
    31261 tccgccgacc cggaaacctc ttacactgcc aaactgtatg ccagcggcac caaacgcatt
    31321 gcgcagaaag tgggcgaaga aggcgtggaa accgcactgg cagcaacggt acatgaccgc
    31381 tttgaactga ccaacgaggc gtctgatttg atgtatcacc tgctggtgct gttgcaggat
    31441 caggatctgg atttaacgac ggtaattgag aacctgcgta aacggcatca gtgagttgcg
    31501 ggctgagcag gtgtgatgtt gttgtcacac ccggagccat tgcccgatgc gcaagcttat
    31561 cgtgcctaca cattatctca acacacgtag gccggataag atgcgccatg aactgcaccc
    31621 caaatgttgg acataatctg acatctgaag gtgcagttta tgaaacattc atttgaagta
    31681 aaacttgccg ctgtaaatca ttatctggc
//
LOCUS       DAZKHK010000051        31666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKHK010000051 DAZKHK010000000
VERSION     DAZKHK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.051"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(61..2055)
                     /gene="cirA"
                     /locus_tag="ACHOS5_002334"
     CDS             complement(61..2055)
                     /gene="cirA"
                     /locus_tag="ACHOS5_002334"
                     /inference="COORDINATES: protein motif:HMM:NF007478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP9777147.1"
                     /translation="MFRFNPFIRHLLSTSIGASLFSFPVFTSAHSTLPDAAEDTLVVT
                     ASAVEQNLKDAPASISVISQDDLSRKPVHNLVDVLRDVPGVQITTDDNRKLVNIRGLG
                     GGYTLILVDGKRVSSRTAMFRNNDFDLSWIPVSTIERVEVVRGPMSSLYGADAMGGVI
                     NIITKKTGKEWSGVISGDMTLQEHRDRGDTYGGQFSAGGPLVENLLGIKVYGALSGRD
                     KDTGKQKSADGQETTRAEGFTTRNGGVDFTLTPLENHEFSAGYSFDRQDRESEGSSDK
                     NRMERQNYSVGHNGQWGLGNSELRFYSEKVNNLNTGNTHPITSWNEVIDGKYVFPFTD
                     YNHIVILGGDYRREKISDPVHLTGEKGSENSTSQYSLFAEDEWRLFAPLALTTGVRMD
                     KHENYGTNWSPRAYLVWNATDSVTVKGGWARAFKAPTLIQQSEAWLTNSCKGGCQIIG
                     SPDLKPETSESIEGGVYYSGQEGLLEGVEASVTVYRNEIDDMIANKRSKKLKEAMTLP
                     NFVGITDDGRPVFRYFNINKARIQGLESELKIPLGEQVKLSLTYTYTDGRDLSSGEDK
                     PLNDSPFHNANGRIDWTPVDDWSFYVSANYHGETRAQSSGTETQGGYTLWDAGASWKA
                     TKNITVRSGILNVMDKDLERNDYSYTVDGRRYFLSMDYRF"
     gene            complement(2743..2946)
                     /locus_tag="ACHOS5_002335"
     CDS             complement(2743..2946)
                     /locus_tag="ACHOS5_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777148.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(3098..3655)
                     /locus_tag="ACHOS5_002336"
     CDS             complement(3098..3655)
                     /locus_tag="ACHOS5_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP9777149.1"
                     /translation="MTEQKRPVLTLKRRTDVETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            complement(3691..4059)
                     /locus_tag="ACHOS5_002337"
     CDS             complement(3691..4059)
                     /locus_tag="ACHOS5_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HFP9777150.1"
                     /translation="MKKILLVAAITGMFVQGVWAKSDQQIRDEAKNNVITVSQVSGMT
                     EGTGVTLNGQIVKQLAAEEDHFELSDASGSIVIDVDDEVWRPLALKAGDKVQVVGEVD
                     ISDEGNRKATVDVLTLERLR"
     gene            complement(4142..4894)
                     /gene="traX"
                     /locus_tag="ACHOS5_002338"
     CDS             complement(4142..4894)
                     /gene="traX"
                     /locus_tag="ACHOS5_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HFP9777151.1"
                     /translation="MTTDNTNTTRNDSLAARTGTRLQSLLVWSPGQRDIIKKVALALV
                     LMVLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNQSRHAHIRQSAINRLWGWAVIA
                     QFAYYVAGFPWYEGNILFAFAVAAQMLKWCEYHSLVWSVSALTLLVLWFPLSSTSYGA
                     VGIILLMFCHRIYRTTHLSVRSMLALCLFPVVALVNLQDSVTAAVSGVLVTYMVVRMV
                     SGIRWPAARFWPADFFPVFYVCHLAVLGVMAL"
     gene            complement(4914..10184)
                     /gene="traI"
                     /locus_tag="ACHOS5_002339"
     CDS             complement(4914..10184)
                     /gene="traI"
                     /locus_tag="ACHOS5_002339"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFP9777152.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPEAGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GQREVTMTVLSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKIPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLESGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLESAISHQ
                     KSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEVREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLSTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGSGDRMRFTKSDRERGYVANSVWTVTVVSGDSVTLSDGQ
                     QTRVIRPGLEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPCAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAQEKPDQPDDKTEQVVRAIAGQEQDRASSSERETALPESVLREPQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(10184..12337)
                     /gene="traD"
                     /locus_tag="ACHOS5_002340"
     CDS             complement(10184..12337)
                     /gene="traD"
                     /locus_tag="ACHOS5_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP9777153.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDNILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDKKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLMAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKSAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQNPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(12393..13124)
                     /locus_tag="ACHOS5_002341"
     CDS             complement(12393..13124)
                     /locus_tag="ACHOS5_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777154.1"
                     /translation="MTSDNIAEYVGENNKFTERPVTEYPPMIQDNTAATKDYLYSNNI
                     DADLLVVIGGLIVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEDCC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAQEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFVQIQYSHESDWSEILRLTDIV
                     LEKVSSGRPAGGYHHRYTGYTWFVL"
     gene            complement(13292..13552)
                     /locus_tag="ACHOS5_002342"
     CDS             complement(13292..13552)
                     /locus_tag="ACHOS5_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777155.1"
                     /translation="MNRAESRLSQVLFLLGYDREKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            complement(13612..14343)
                     /gene="traT"
                     /locus_tag="ACHOS5_002343"
     CDS             complement(13612..14343)
                     /gene="traT"
                     /locus_tag="ACHOS5_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP9777156.1"
                     /translation="MKMKKLMIVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(14392..14871)
                     /locus_tag="ACHOS5_002344"
     CDS             complement(14392..14871)
                     /locus_tag="ACHOS5_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFP9777157.1"
                     /translation="MKHLAHIALITVIQLIACNLADWGNAETVFILCFIVLWQGLFIW
                     LFSQIRKKRHVSNEFKFSKGIWYILMPASSLLSPLLSLMIFTIGTLYELRRVCGCVSV
                     REWMQSQVNEKTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYDE"
     gene            complement(14893..17736)
                     /gene="traG"
                     /locus_tag="ACHOS5_002345"
     CDS             complement(14893..17736)
                     /gene="traG"
                     /locus_tag="ACHOS5_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFP9777158.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVRVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLHSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVAIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNKLTLSVLKGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLAATAGYISAMIPPLSWMMVRGLGAGFSSVYSHFASS
                     SITPTASAAGSVVDGNYSYGNMQTNNVNGFSWSTNSTTSFGQMTHQTGSGATATQTRD
                     GSMVMDASGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRST
                     RASAGMYGDAHAEWGIKPKILGVGGGAGVRGGGRAGIDWEDNDAHTASSNTQSSHNAR
                     HDIDARATQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTN
                     MTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRR
                     AMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQ
                     DSNIRNDVKQQVDNMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNK
                     YNEEKSAQEGMPGADSPQELMNRAKEYQDKYKQ"
     gene            complement(17733..19151)
                     /gene="traH"
                     /locus_tag="ACHOS5_002346"
     CDS             complement(17733..19151)
                     /gene="traH"
                     /locus_tag="ACHOS5_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFP9777159.1"
                     /translation="MNLFLSPTLKHKLCRNRGGIPALLLCSCLSFIPFTAVADVNSDM
                     NQFFNKLGFATNTTQAGVWQGQAAGYAYGGSLYARSQVKNIQLISMTLPDINAGCGGI
                     DAYLGSFSFINGEQLQRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMASDINS
                     MNLSSCQVAQGIVGGLFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVR
                     DKASDKDKERVLKNINIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPA
                     RTTDRDILRAIMEGGTAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQN
                     KAVSDTPLDDREKGFISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQE
                     LLQQARAMIATGNYPQTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMR
                     QQLSARMLSRYQNNYHFGGGTL"
     gene            complement(19539..20057)
                     /locus_tag="ACHOS5_002347"
     CDS             complement(19539..20057)
                     /locus_tag="ACHOS5_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777160.1"
                     /translation="MAGQQIFKRSVSCISLLSAVASGFYLLWSEWSAVKCQFDGMIFL
                     SFRNAGLFLLFFIILLIVVLFSVSMGVSGRYAVNPQIVTPGIIISLLLQCSAIMMVQA
                     WRWSPYALAGTFLLVQFWSGYLTGWVQRDLHEGRVKTGFRNTVSEFWLLIPLVLSGWG
                     ISWGYYTTLFTS"
     gene            complement(20073..20615)
                     /gene="trbB"
                     /locus_tag="ACHOS5_002348"
     CDS             complement(20073..20615)
                     /gene="trbB"
                     /locus_tag="ACHOS5_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFP9777161.1"
                     /translation="MFPVKSLSVVLLFLASCTQASTRAEIERLWHPEGMAARTTQPAA
                     DAQARQEKTATRWFRLSNGTQVNLADWKVVLFMQGRCPYCHQFDPVLKQLALQYGFSV
                     FPYTLDGQGDTAFPQALPAPPDVLKTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGNKVSQ"
     gene            complement(20602..20892)
                     /gene="traQ"
                     /locus_tag="ACHOS5_002349"
     CDS             complement(20602..20892)
                     /gene="traQ"
                     /locus_tag="ACHOS5_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFP9777162.1"
                     /translation="MISKQRFSLPRLDITGMWVFSLGVWFHIVARLVNSKPWMAYILA
                     QVISAVLVLWGAYRVLDTWIARVSQEEREALEVRQQLLLRERKDNDDEHVSR"
     gene            20974..21309
                     /locus_tag="ACHOS5_002350"
     CDS             20974..21309
                     /locus_tag="ACHOS5_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFP9777163.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSFPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYVYFCELFRSHWIAVWFILWSVVINLTIGFVIYDK
                     LAPVISLMK"
     gene            complement(21290..21634)
                     /gene="trbA"
                     /locus_tag="ACHOS5_002351"
     CDS             complement(21290..21634)
                     /gene="trbA"
                     /locus_tag="ACHOS5_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFP9777164.1"
                     /translation="MSEDSLNIVTGIVLLIFIIIVGYFFSERNDRKIFFLSSLVFLVI
                     NILCLYVLTARFWFLCGAVMNQCAVPVVSMAVGTMPVITKFDRFRRIFVCIMLSSAWS
                     GVMWFFIISLVK"
     gene            complement(21766..22131)
                     /locus_tag="ACHOS5_002352"
     CDS             complement(21766..22131)
                     /locus_tag="ACHOS5_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777165.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCTLSMM
                     MAEGIVIMKYELWHIPPALPGLAGVLSWLLLLLHTGYFSGISEGVHKRLWLAVAAVFS
                     GAGMIIFYWLLQPSETIMK"
     gene            complement(22145..22888)
                     /gene="traF"
                     /locus_tag="ACHOS5_002353"
     CDS             complement(22145..22888)
                     /gene="traF"
                     /locus_tag="ACHOS5_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFP9777166.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(22881..23138)
                     /locus_tag="ACHOS5_002354"
     CDS             complement(22881..23138)
                     /locus_tag="ACHOS5_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFP9777167.1"
                     /translation="MKVIFTSNRLVDFLIRLVITAIVISPVIIWSWDTVKETTAGGVL
                     VAAFVILYSGVLLFILYFCFSALTDLQKADERKSDERNEDE"
     gene            complement(23152..25056)
                     /gene="traN"
                     /locus_tag="ACHOS5_002355"
     CDS             complement(23152..25056)
                     /gene="traN"
                     /locus_tag="ACHOS5_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFP9777168.1"
                     /translation="MKEKINTFFRSLNVNDSGRPRNICAIVFFLSFSVFADSNSDYRA
                     GSDFARQIQGQGIGSIQSFNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWA
                     TSETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEF
                     TNYTCERDTMVEEYCSRTARITGAWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPV
                     AGTVESASLSAHAGLYFLNSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLT
                     GSGCTANGNCLPHGNGDRMVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLKGTECSEPGETKTGVTEGKPWSITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLVSRQCAFYSDEGSCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQ
                     IAGQLKQVGQ"
     gene            complement(25150..25530)
                     /locus_tag="ACHOS5_002356"
     CDS             complement(25150..25530)
                     /locus_tag="ACHOS5_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777169.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLLP
                     YIAFACPVKMAALLEQRLRKDAESAIPRLTWGWSVKGAFNSDLQLCFRLQPEGPHCWY
                     HRYCRRSLRADFRTLILLLKSLSE"
     gene            complement(25527..26165)
                     /gene="trbC"
                     /locus_tag="ACHOS5_002357"
     CDS             complement(25527..26165)
                     /gene="trbC"
                     /locus_tag="ACHOS5_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFP9777170.1"
                     /translation="MNKKIYILFALTAGLFLSAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHADNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALAVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKPQ"
     gene            complement(26394..26726)
                     /locus_tag="ACHOS5_002358"
     CDS             complement(26394..26726)
                     /locus_tag="ACHOS5_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777171.1"
                     /translation="MEKKLSSKYIHIYMSISCVFIRLTGKALKLFPFALVMLFTVLFY
                     FMPENVISDIISEWQRSDVSDRIRVIRLYLGTTFLCTWAAVFLECLCSDEFLPGKRLN
                     KNEEENNG"
     gene            complement(26753..27745)
                     /gene="traU"
                     /locus_tag="ACHOS5_002359"
     CDS             complement(26753..27745)
                     /gene="traU"
                     /locus_tag="ACHOS5_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFP9777172.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWNCIFP
                     LSLGSTKVSQGKVPDTINPSMPIQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGITIPELGKTGQGTAKKDDKQVNGAFYHVHWYKYPLTYWLSIITSAGCLMGGDMD
                     IGYISEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNSIGVDVAVCYEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(27742..28374)
                     /gene="traW"
                     /locus_tag="ACHOS5_002360"
     CDS             complement(27742..28374)
                     /gene="traW"
                     /locus_tag="ACHOS5_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFP9777173.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGISRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQIPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(28371..28757)
                     /gene="trbI"
                     /locus_tag="ACHOS5_002361"
     CDS             complement(28371..28757)
                     /gene="trbI"
                     /locus_tag="ACHOS5_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFP9777174.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEAQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(28754..31384)
                     /gene="traC"
                     /locus_tag="ACHOS5_002362"
     CDS             complement(28754..31384)
                     /gene="traC"
                     /locus_tag="ACHOS5_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFP9777175.1"
                     /translation="MSNNPLEVVTQAVNSLLTALKLPDESAQANQILGEMNFPQFSRL
                     LPYRDYNQESGLFMNDSTMGFMLEAIPINGANETIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRHLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFRQLKEAGVVQRAESFNVVNLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERIRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGRYFNSDKPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
BASE COUNT         7471 a         8960 c         7208 g         8027 t
ORIGIN      
        1 tcttgtttat tttcagaaca ttatcttata aaggctcctc ttggagcctt cggtttacta
       61 ttaaaaacga taatccattg acagaaaata tcttctcccg tcaacggtat aactgtaatc
      121 attccgttct aaatctttat ccataacatt taatatacct gaacggacag ttatattttt
      181 cgtagccttc caggaagctc cggcatccca taatgtatac cccccctgcg tttctgtgcc
      241 ggaagattgt gcccgggttt ccccatggta attagctgat acgtaaaaag accagtcatc
      301 cacaggagtc cagtcaattc ttccgtttgc attatgaaac ggactgtcat tcagtggctt
      361 atcttctccg ctactcaaat cccgtccatc tgtataggta taggtgagag acagtttcac
      421 ctgctctccc agcgggattt tcagttctga ttcaagcccc tgtatcctag ctttatttat
      481 attaaagtat ctgaaaacag gccgtccatc atccgttatc ccgacaaagt taggcagcgt
      541 cattgcctct ttcagtttct tactgcgttt gttcgcaatc atgtcatcaa tttcattacg
      601 atacacagtg acacttgctt ccacaccttc caggagtcct tcctgtccgc tgtaataaac
      661 accaccctca atactttcac ttgtttccgg tttcaggtcg gggctaccga tgatctgaca
      721 tccccctttg catgagttcg tcagccatgc ttcactttgc tgaatcaggg tcggagcttt
      781 aaatgctctg gcccatcctc cttttacagt aacagaatcg gttgcattcc atacaagata
      841 tgctcttgga ctccagttgg ttccataatt ttcgtgctta tccatacgaa cccctgttgt
      901 caaagccagg ggcgcgaaca aacgccattc gtcttctgca aaaagtgaat actgacttgt
      961 cgaattttct gaacctttct ctccggtaag gtggacagga tcacttattt tctccctgcg
     1021 ataatcacca cctaatatca cgatatgatt ataatcagtg aatgggaata catattttcc
     1081 atcaataacc tcattccatg aagttatcgg atgtgtatta cctgtattca gattatttac
     1141 tttttcactg taaaatcgga gttcactgtt ccctaatccc cattgtccat tatgaccaac
     1201 agaataattc tgtcgttcca tccggttttt atcactacta ccttcagact cccggtcctg
     1261 acggtcaaaa gaataccctg cgctgaactc gtgattctcc agaggagtaa gcgtaaagtc
     1321 aacgccacca ttacgggttg taaaaccttc agcacgtgtg gtttcctgtc catctgccga
     1381 tttttgtttt ccggtatctt tatcccggcc acttaaagct ccgtaaactt taattcctaa
     1441 aaggttttca accaaaggcc cacctgcact gaactgtccc ccataagtat cgccacggtc
     1501 tctgtgttcc tgtaacgtca tatcccccga gatgacacct gaccattcct tacctgtttt
     1561 tttagtaatg atattaatga cgccccccat cgcatccgca ccatacagtg atgacatagg
     1621 gccacgcacg acctctacac gttcaatagt ggaaacaggg atccagctca gatcaaagtc
     1681 attattccgg aacatagctg tgcgggaact gacccgttta ccatccacaa gaatgagggt
     1741 gtatcctccg cccagtcccc ttatatttac gagttttctg ttatcatcgg tggtaatctg
     1801 caccccgggc acatccctta aaacatccac cagattatgg actggcttac gggataaatc
     1861 atcctgacta ataacactga tactggcagg tgcgtctttc agattctgtt caacagcaga
     1921 tgctgtaact accagagtat cttcagcagc gtccggaagt gtcgaatgtg cagaagtgaa
     1981 cacaggaaat gaaaataacg atgcacctat gctggttgaa agaagatgac gaataaaagg
     2041 gttaaatctg aacatttctg attatatcca taatttgtat tcaatgttgc aagatttacc
     2101 cacactgact tccataaata tgatggagtt tgccccatct tatattaaga atattgatat
     2161 acttctttga ttaaaattta tttattgata tttttatgtc aataccattc ttctctactt
     2221 tactatacat actttcctgt tcgcaaggtc atgtattccc gttccccgtt cgtaccctat
     2281 cggcttcccc ctgctacata ctactttgtg atacagatta tatttttcac ccctgactat
     2341 taaacattcc tgctcttgct gataggataa ctgtgttata tcgcaggcta ccggatgtgg
     2401 cgctgaatat aacggtcata gataagtcat acatgccctg ctcaggggat tagcgataag
     2461 acggaccggg agagtcatct cccggttttt ctttatcctg ccatgaatac tgcccggctg
     2521 tctgtcagat tcaccggata accctgctgt tatgcattcc actgtcacct cctgcttctt
     2581 gaaattcatc acatttttta tccctgctgg cttacccggg atgtgaaata tgttaactct
     2641 gtgatgtccg tttgaaagac agggcaactc cttcttacac cactataata ataataacga
     2701 tctgccacct gatcccccgg agctccgcga cgggggttat ttttatcggc acttacaccg
     2761 gcatgcagat gccctcttca gggcaaaatc ccgccatgct tcacagggtg ccagccgggc
     2821 cgcattctgc caggcctcag cggcttccct gaaaaaacca cgaatctcct gacggacggc
     2881 atgacgcccc acgcgctgat agcgataaat accgctgtgc tcagataatc cactggatac
     2941 agccattttt tatcccccct cttgacccgg ctcccggatt atgtcagaaa cattctgaaa
     3001 catccctgaa cggttgggtg ttttctccat caaaactctg gatttctgtt ttcccgattt
     3061 tttctcgctg ttgccctctg tcatacagag ggctctttta ttccgccagg atcgcccgca
     3121 gctcatcctt gatccggttc tgtctgcgca ccttttccag gcgcgcctgc gcatactgtt
     3181 cttcttcctg tgtgatatgt tctgtcacat agccttccgt atcatagcga caggcgccgg
     3241 ctttcattgc gcacagataa ctttccgaac gggtgatggc tttcagcgcc cggctgagtt
     3301 ttttatgaga gagcgggata ttccgctgaa acacctcatc cagtaaaacc tcccggatac
     3361 cacaggcaag aagcctgggc gtgttaccgt caaacaatcc cggccaccag ggtttcagag
     3421 tgttcaccgc ctcatccagc gagggcagcg tcagataaat ggacagcgcc tgtctggcct
     3481 gtgctttctt cgccgccagc tctgcttccc gggcagcctt ctctgccagt ttctgctttt
     3541 tcaccttcca ttttggtggc gtggtgacat tgatgatggt tttccggctc cgggtcggtg
     3601 tttccacgtc ggttcttcgt ttcagtgtca gtactggccg tttctgctct gtcataactc
     3661 acctgccctc accggtatcc gttgttcata tcagcgaaga cgctcaagag tcagtacatc
     3721 caccgtggct ttccggtttc cctcatcaga aatatccact tctcccacaa cctgaacttt
     3781 atctccggct ttcagggcca gcgggcgcca tacttcatca tcgacatcaa tgacaatact
     3841 tcctgatgca tcactgagtt caaaatgatc ctcttcggct gccagttgct tcactatctg
     3901 accattcagc gtcacaccgg tcccctccgt catgccactc acctgagaaa cagtgatcac
     3961 attattttta gcctcatccc ggatttgctg atctgatttt gcccataccc cctgaacgaa
     4021 cattcccgta atggctgcca ccagtaatat ttttttcatc atctgctcct gttttttcct
     4081 gaatgcgttc tgatttaaac aaaaagactt tcacagttca tccgctttct tgcgcttcct
     4141 gctacagggc catcactccc agcacagcga gatgacagac ataaaatacc gggaaaaagt
     4201 ctgccggcca gaaacgggca gcaggccacc ggataccgga taccatcctg acgaccatgt
     4261 atgtcactaa cacaccactg acagcggcag tcacgctgtc ctgcaggtta acgagagcaa
     4321 cgaccgggaa aagacagagc gccagcatgc tccgtacaga aaggtgcgtc gtcctgtata
     4381 tcctgtgaca gaacatcagt aatatgatcc cgacagcacc gtaactggtg gaagataacg
     4441 ggaaccagag aaccagaagt gtaagtgcac tgactgacca taccagcgag tgatattcac
     4501 accatttcag catctgtgct gccaccgcaa aggcaaaaag gatattcccc tcataccagg
     4561 gaaagccggc aacgtaatac gcgaactggg caatcacagc ccatccccac agccggttaa
     4621 tggccgactg ccggatatgc gcatgacggg actgattcag tccccagacc agcgcaaaca
     4681 gcggaaacgc gccccgcccg gccagaaaca tccacgtctg ctccagatgc agaatgcggt
     4741 ttgcatggtc cagcaccatc agcaccagtg ccagtgccac ctttttgatg atatcccgct
     4801 gtccgggtga ccagaccagc aacgactgca gcctggtacc ggtccgggca gccagtgaat
     4861 cgttacgtgt cgtgttcgtg ttatccgttg tcatcagcct ttgtcttcct gtatcagtct
     4921 ccgcccaggg ttttctcttt ctgcaggtca cggaccatat cccgctccat ctgctgaagt
     4981 cgctcctgca gcagattttc ccgggcaacc tcccggacag cgtcctgcac ccgttgaggc
     5041 tcacgcagca cactctccgg cagcgcagtt tcccgttctg atgacgaagc cctgtcctgc
     5101 tcctgcccgg caatagccct gacaacctgc tctgttttat catccggctg gtcaggtttt
     5161 tcctgtgcca tttttctgac aatctcatcc gcacggcgtt ccgtttcacg acggatggcc
     5221 tcttcagcct gccgctgtgc cagcacctct gccgtgaccg gctcaccgtt tccggccccc
     5281 ggctggacgc tgttgtccgg gatatccccc cacacgcgac caccggtgat ggcacaggga
     5341 ttccacggac gaccttcacc ggcgattctc accaccacac cgctgtcggg gttatcacgg
     5401 gcaatccgga caccatcctg catattatcc gccagcaggc tctcgccgtt acggctgccc
     5461 tgcagggcca caaactgtgc atcttcactg cccttcaccc ggccttcacc actgaatccc
     5521 cgcagcccgt ttccgtcatc cgtggtcagt gggttcagcc agataccggc ggacttgccg
     5581 ttacggtcaa aagccggcag ggccacatac ggctgcggat atttgcgccc cggtgcaata
     5641 aaccgtgcag gactgtctcc cccggccagt cccgcctggc ggagaacagc acggccagct
     5701 gccacatccc gcagctcccg tgccgtactg aacagccgct ctgcattcat gacctcccgg
     5761 tccggtttcg gctccagcac atcgtgggca gttcctttct gtacggcatt gttgatggca
     5821 tccgtccagc cctgacggtt atcggtgtac acctgcacat gctgcttcat acgcgacagg
     5881 gccacgtagg ctgactcaaa gccggccatc agtttccggt taccttccgt gccttcaagc
     5941 gcgatggcaa aggtttcact cgccccctgc gcaccgtgag cggtgatggc ataggccagg
     6001 tcaatatgtt gctctgcctg ctccaggccg gggcgaatca cccgggtctg ctgtccgtcc
     6061 gacagcgtga cactgtcacc ggaaaccact gtcaccgtcc agacgctgtt ggccacatag
     6121 ccgcgctccc ggtcactctt cgtgaagcgc atccggtcac cgctccccac ccggatggtg
     6181 tccggagtgt acagtgtgac gccttcagcc accgcctccc ggggcgaaat cagccgcgtg
     6241 ttaccttccg catcctccag ggttatcagc ccgtcatcct tgctgatacc ggcaatccgg
     6301 tgatacacat tatccaccag ggcaagggca tccgggttat tctcccaggt ggagagacga
     6361 cgcagctctc cgtcacgtat attcgctgtg ctcaggacag gcaccatgac ctgctctttc
     6421 cccagctcac cggccttttc ccgtgcatca tgaatcatgc tgttcagtac gcgcctgtct
     6481 tcattcaggt gcgtaacaat cagcgtctgc tcccgtactt ccggtgtcct gccggtatag
     6541 tcgcggacaa tggcttcata cagtgtcatg gggacatccg ggaaagcctc gcctttcagc
     6601 atcgccttct gctgcgcttc tgccagtttc gcttcctggc tgtgactgaa ctccgtcacg
     6661 gagtgctccg gtgcccatgc accctccaga cgtggcacct gagacggttt cacactctca
     6721 agcccggaca gtgccctttc cacatcccgg ttaatcaggc tgtataccgc ctcccgcagt
     6781 tccggcgtct gacgcacaat ctctttcatg atggccacat cggcagcact gcgcgtctgc
     6841 tggagacgga aaggctgacc gggcgcgatg gcctgcagct ggtccgtgtc accgctggcc
     6901 acggcacgac caccaccggc cgcaatcagg gcgtatgccc gtgccatgtc ggtattgccc
     6961 accatagagc tctcatcgag caggaacagc gtgttgctga aatccggcgt ttctccgctg
     7021 cgctgctgca gctgcgtgtc atgcagaaaa gacgccagtg tctgcgcatc cacgccagcg
     7081 ctgcgcatct ccccgaccgc acggtgcgtg ggccccagcc ccacgacacg gggacgctca
     7141 ctctccggca gcatcttcac ggctgacatc accgcccgga actgtgtggt cttacccacg
     7201 ccggcataac cctgcaccac cgtgaaacgg tcggacgttt ccagtatcat gcgggtggcg
     7261 gcacgctgcc ccgacgtcag tgtctccatg agttcgccgg gtaccctctc catcagcggc
     7321 gtgaccgcct ccttaccttc gagaatatgg cgaagaatgc tcttttctgc ctcatacgac
     7381 gcacgggaaa ccagcaggcc tgtgccatag ccttttgcca catccacata cagtaaatca
     7441 ccgcgtttta tctgcgcatt gatttcccct cccagctcag taaaactggt tccttctgca
     7501 gcaaacgact tcgcctctgt cagcaggtcc accatgctga aggccaggtt tttactttcc
     7561 agcacaggaa gggccagatg aatggcctgc tgtgccggag tgtgaagtgc ggacttctga
     7621 tgactgatgg cactttccag caatgtctca ccggcacgcg cctttatctg ctcagaaacc
     7681 accgtaaagg aggggtgacg ggcaagtttt tccgcagtac gggtttcatc cagtgaggaa
     7741 tacagccgga catcacgacc gctgcgggcc agaccgttca gggtggcgtt gtccattgcc
     7801 atctgtgtga cggaggcaaa aaccttcgca ctgtcactga cggaatgccc gggcgtttcc
     7861 acccagccgc tctccagctt cagtgccgtg aacggtgaat cgctcacagg cagggtggcc
     7921 ggttcagccc gccccggcac aacaaccgtc atcgcatctt cactgacgga tgccacctgc
     7981 aggcggtcac cgccggagac gcggagtccg ggaattttcc ctgtcaccct cagacgctcg
     8041 ccgtctgcca ccggtatttt ttccggccgg aacaacgacc agctgctgtc cagggaggat
     8101 atacgcacca cctgcgtttc gccctgcgca tcccgcaggg tcaggctgtg actctgcgct
     8161 gtcacccggt cgataacata gcggtcatga ctgcgtgtct ccgggttcca ctgctccatc
     8221 accatccccg ggcggtacat atcccgcaga taacgactcc ggctgtccag ccagaccggt
     8281 gacagcaccg tcatggtcac ctccctctgc ccgagtacgc cctgcgtttt cagttcactg
     8341 cgaatggcct gtgtcagtat ggcctgttcc cgtactccgc tgacctgtgc cacgctctct
     8401 tctccggctt tcacgctggc cgcaaaatct ccggccagcc gggcatagcg gacattacgg
     8461 tccggttcac tgatgatggt tgccggtcgc tgctctcccc cctgccagcg atatatgttc
     8521 accccggcat ccttcatggc catcagcgca ctgcctgtac cggttcgctg cccgctgtcg
     8581 gttatcagga cctgtacgtt atgacgtgcg gcaccgtcca gcagggttaa cgtctctttc
     8641 agggagagtt tttcgccctg gtcgacgata acggtactgc ccggcgtgaa ggccatgcct
     8701 tccagcagct gacgacgtcc ggttatcagt tcaccggaca gccgttcatc ctgcttcagg
     8761 ttcatctgtg agcgacggtc agcggcaata atctgcacct cccgcccctg ctcccgggcc
     8821 atcatgacca gttcagccac ccgctcacgc tgcccggctg caccgccctg cccggacaca
     8881 atggccagcg acgggcgatc ctgtgccaga acactgaccg catcgctgta accggccgtc
     8941 cggggaacac ttttctccgg atgtacagtc acccggttct gtttcatgat gtcacgactg
     9001 agtgcccgga cggacagctc atcgagcaca tgaatcccgg acgtgaacag ccccttctca
     9061 cggtcgaggg ggataagctg ctcacggctg atggcctcat cgataccggc acgcgcccgc
     9121 tcaatcacac cggcttccgg cggcagtatg ccgaccgtcc tggccagcac gtccgtgtac
     9181 gtgaactgca cttttcgttc acttaatccg gcaatggcct gtgtcaccgc ctgctgcaca
     9241 tccggcccgt cctgtgaagc gggtccgggt gtctgcgtgc gggtctccgc ccgttggtct
     9301 gccgcgtcac gatacgcccg gatgtcgaac ccggtttcct tcagcgtctg catccattca
     9361 gccattctga cttcaggatc gacgtgctgt ttggattttc gcgtatccag tgccgccaca
     9421 tcccgggatt tcagcgaggc gtcttccccg acggcctccc ggatagcctg tgagcgtccg
     9481 gaaaaggcct ccaccggtac gcccggcatc tcccacatac cgtgcttacc caccacttca
     9541 gtttcatagc ccagcgcctc aacgcgctga cgtaactcac tctgatatat tttcccgaac
     9601 gcaatacggt tcgcgagcac gttctcggaa aaaccggttt tccccacttt atcactgctc
     9661 agtgtcttcc attcgccgtt atgctgcgtg acattagcca ccaccgcatg cgtgtgtaac
     9721 tgtgggtcct gatcgcgact ggtgtcgtgg ttaaagagtg ccatcaccag attaccggtc
     9781 agtaccgttt ctgactgtcc gtccgtcatc acccgtgtgg aagccagcgc ctccacctga
     9841 cggacagcaa aatccacggc ctgattatgt gcatcaatca ggcgcttatc gccacctaac
     9901 atggccatca tggagacact tttgggggcg gagaaggtca ggtcatagcc gggacgatgc
     9961 ttattactgc catcctgcat acggcttaaa tccgccccgt ccggcagctt cccctccaga
    10021 agacgggtaa agacatcctt atcaacactg ccctgcagcc caagctgttc agcccccttg
    10081 cccgcccagc gttcccccat gctacccagc acatagtaat tatccttgtc ggtgtaatag
    10141 tttccggcac tgcctgccga tttaacaacg ctgaaactca acatcagaaa tcatctcccg
    10201 gctcaacatc ctccccgcgc tcccggtgca cgttgatatt cacctcttca cggcgctgca
    10261 tctgctgctg gatatccgga ttctgctccc gttgccatgc ctcataaacg gccatatcca
    10321 ccacttcacc ggattcactg atcccgggtg gcagttgctg ttccatctct tcttccggtt
    10381 tcattttcag ctcctgctcg atcccccctg ccggaacatt cacacctgca tctgacttct
    10441 tatcgttgat ggcaggagac accggctgtt gcggctgttc agcctgagtc acgtcttctc
    10501 cggaaacaac ctccgggaca tccggttcga agagactggc catctgacga ccttctgctt
    10561 cccttgcggc aagtacggca ctcagacggt tctccatttc aggattgatg tcacgcggaa
    10621 taaactccgg agcaaccttc ggtcgtgcct gatatttcag ggagagtttt actgccggat
    10681 acggtccggg cagggtgaca taacaggtca gatccggcag agactgaatg tcggaataac
    10741 tgaccagcgt ctggcgctcc atatccttac cggtcgatac cccgtcacgt accggatcag
    10801 caccgtagga atactgctcg ctggctttca ggtgctcttt ctcaccaatt tcaccggcag
    10861 cgaactctgc aatcttatgg ctgggggaac ggaaaaaggc acgggtgttc atgacgtcaa
    10921 acagcgaggc tgccgatttc tcaccgtaga tatcttccag ctgggcatag gactggatac
    10981 cgaacacata acagccaccg aacttacggg cttccggcag gatctccacc aggtccggca
    11041 gtttgtgcag cgtgggtaac tcatcacaga agaaccacac acgacggtta cggttctccc
    11101 ccatcgccat caggccacga atggcgatgg acagccacat ggagatcacc ggtttcaggg
    11161 aagcatgggt gtcggcattc gacgaaataa acagccagcc gtttttctta tcttcacgga
    11221 caccccgcat ccagtcacga atggtgaagg actcaccatt atgctcaatc ccctgcaggt
    11281 aacggatggc tttcacgtag ttggtcagca cagcacggat ggaaatcgcc gttttctcga
    11341 ttttctcttc caccaggttg gccgccggtg agttacgcag gaaggtacgc agtttttcaa
    11401 ttttgatgga aagcagtgtg tccaccagtt tgctgtagct gcggttgggg tcattgcgca
    11461 tcaggtacgc agcttccgca aaaatggtgc gtcctgaacc ctgccagaac gggtcctctt
    11521 ttgtgcccat cgggatcagg gtatttgccg tattatcaaa atccggctgt gtcaggcact
    11581 ccttccacaa atcccaggcg gcacagcggg catccagcgg attcaggata ttatcgatgg
    11641 acgggtcata gtaactttta acaaattccc ctgatcggtc ataaatcacc accatatcac
    11701 cgcgctgacg ggcgtagttg gccagacgac ggataacttc cgacttaccg gccccgaccg
    11761 tgccgtgcag acagaagttc tggatttcag aatcccggat aatcggcaga tcgccaatcc
    11821 ggatatcgga gtccttgccg tcttttttca gcatcctggc aacctctttc ggattgtcag
    11881 tcagctgacg accacctgtg acttcatttt cactctgctg tttaccctga cgtcccagaa
    11941 tccaggagac aacaaagaag gtgatcaggc aaataaccag ggccacgaca gacgccagaa
    12001 cgaatgcaga ccatagctgc tcaccgcacc agatcatgta tttatcatgc agtacctgag
    12061 ccgcgttcat ccggaaggtt ttgccgtaat actggatctc atagaccggc tgggacctga
    12121 ttaaatcccg catgccttcc agcgtggtgc accaccagta aatacagccg ttcacaaacg
    12181 tctgccagct tatttttacc cataaaacca gaccaacgag gatccagaaa aaaataaaca
    12241 ggcagtaaag catgatattg gcgatctgac tgaacatgcg gatacgcatg gacgcaatct
    12301 gaccgccctg ggtcatatcc tttgcgttaa aactcatgat gaaactccgg gaaagtcagg
    12361 aacagagcag aaaagagtgg tgtggaatac attcacagaa caaaccatgt ataccctgta
    12421 taccggtggt gatatccgcc tgctggcctg ccggaagaaa ctttttccag cacgatatca
    12481 gtcagacgca atatttcaga ccagtcactc tcgtgtgaat actggatctg aacaaaaagg
    12541 cgtacccgct cacgataatc cagagccgtg cccggttcat aaaagtggcc actgtcagga
    12601 gaaacacggg acgttttatt gatgagatcg tatttatcgg catgagtctg tgacgacaga
    12661 gaccaggtaa tattttcttc ctgctcacca tcaatacggc tactcccggg cgtccaggca
    12721 atttcctgcg cactggtcac gatgatctgt ctggagactt ctgccagctt tttgggtgta
    12781 cagaataccg gggccagcac acacagggac aacaaattac agcagtcctc ttccggctta
    12841 agcggattgc tcccggattt ctcaggaaaa gatatttttg tcagtatttc agttaattcc
    12901 ttatgcctga gagaattaat aaagtacatg acaagcatca tgagaaacaa tataacaata
    12961 agccctccga taaccactaa aaggtcagca tcaatattat tgctgtacag atagtcttta
    13021 gtggccgctg tattatcctg tatcatcggg gggtattcag ttacaggcct ctcagtaaac
    13081 ttattattct caccaacata ttcagcgata ttgtctgatg tcataatgac tttctccgga
    13141 agcttccggc ttcgccggca acacttcacg gtcgcacaaa gaatttgcac aaccgttcag
    13201 tgttctcata tttatttcgg aaacgccggg aagtgacaaa atttattttg ttactttccg
    13261 gggttggaca acgagcaacg cgaggcgtca gctattttct tctgcgacgt ccggtcggat
    13321 attcactcac aggaagatat tctgttgcgc ttacgccgat ctcatatgca gagaacggtt
    13381 ccagtgctgt caggtatctc tggcaggcta tggccagttc agaatctccg gttgcatcag
    13441 caaaccggta aagttctttt aataactccc gacatacatg ctcaggatac ttttctctgt
    13501 catagcccag cagaaagagt acctgcgaga gacgggattc tgcacggttc atgtattcat
    13561 ccggggcagg aatatcggcc cggtacaatc tgtccgggcc ggatgggtaa tttacaggat
    13621 gttcgcgatg gatttagcca gctgatcttc cagtacaggc ttcgcttctt caaatttcag
    13681 gttaaccttg ttggcattag ataccacacg ggtctggtat ttatgttgat ttccggttga
    13741 tgtgctggtc tgcacttttg caccggaggt tccctgacgg agagctgcaa cattatcagt
    13801 tctgacttta gatgttgtcc gctctgcaat ctgcacatcc gtgatcatgg tgtagttgat
    13861 atcctccacc attgcatcgg cagccatacc aatcaaccct gtggcaatcc ctacacccag
    13921 cgccgcaccg gcagagctgg agttatatcc tgtgatgccg gtgcccaatg ctgcaccggc
    13981 aatggcacct tcataaccgc tgttcagcca ggtctgggac tcccgcaaat ccattttgtc
    14041 tgccttcagg acattggcct ggatccagta gtatgccttg tcaggtgaag tcacaacctg
    14101 gtagcctttg gctttaacgg catcactgat ttttgcctgc aatccgctca tatccttgtc
    14161 agatgtgttt ttgatttgca ggaaaacggt gcgttcactg gaaggctcca gccagatggt
    14221 ttcactcatc tgagttttga cttcaagatt acgcttcttg attgctgtgc tcatcgcacc
    14281 acaccctgaa aggaccagag tggaactgac cagggtaact atcatcaatt ttttcatttt
    14341 catcaatgtt ccttcatatt gaataaaaca ccataatata caaattaaga ttcactcatc
    14401 atatcgtttt gaactataac catatgaatt accaccgata tccacaccaa tacctgacat
    14461 aggtaaccct gttgcaggat tagtacgatg aaaatccata ttatcaaaat ccagatgcaa
    14521 atcttcatta gttttttcat ttacctgaga ttgcatccat tctcttacac ttacacaacc
    14581 acaaactcgc cttaattcgt atagtgtacc aatggtaaaa atcatcaggc ttaataacgg
    14641 cgagagtaag gaagatgctg gcatcagtat ataccagatg cctttactga atttaaactc
    14701 attcgacaca tgccgttttt ttcgtatttg cgaaaacaac catataaaca atccctgcca
    14761 tagtacaata aagcacagga taaaaactgt ttcagcgttc ccccaatcag ccagattaca
    14821 ggcaatgagc tgtatcacag ttatcagagc aatatgagct aaatgtttca ttatcatctc
    14881 ccaaacccag cattattgct tatacttatc ctgatactct tttgccctgt tcataagttc
    14941 ctgtgggctg tcagcaccgg gcattccttc ctgagctgat ttttcttcat tatatttatt
    15001 attctgagat aatgcctctg ttttatggtg attttgcagt tcagaatact gccctcttac
    15061 aatattttcc tcgccgtgga tgctgttctg agtatctccg atattgcccc gatattctgt
    15121 gaccatatta tcaacctgct gcttcacatc gttacggata ttactgtcct gcgttctttg
    15181 ctcaataata ccctgatgcc cctgatggtc tgcaataata tcctggctgc caccgcccga
    15241 aggtacgctc tccattcctt taccgatatc gccacgggat tcacgccagg cgttatcaac
    15301 accaggctgt acctgttcct gaacaaaaga ccaggccatt gcccgacgac gttctgcaat
    15361 ctccggtgaa ctggtgtttg tcagaatagc ttctgcatcc tgcggcgcgt gtttcatcac
    15421 atactgtgca aactgttgtg acaggtcttc actcatctgc ccactcatgc tttcagtacg
    15481 ggaggccatt tcagcatatt catggctgcg ggtcatattc gtcgtgtact ggtcgtaact
    15541 ctgtttcgct gagttcaggg ctgcagacag ctggtccacc ctggaatcgg cattattgtc
    15601 agtatggctg ccggattcac tgaccttgcg actggtaaag taatcgctgg cctctttaaa
    15661 gtcttgtgta gctctggcat cgatatcatg acgagcatta tggctcgact gagtattact
    15721 acttgccgta tgcgcatcat tatcttccca atcaatacca gcccgaccac cacctctgac
    15781 acctgctcct ccaccaacac caagaatttt aggtttaatc ccccattcag catgagcatc
    15841 tccatacatc ccagcagaag ctctcgtact tctggatgcc agctcctgag tcgcctgttc
    15901 attactgata ttatgcgcct tcgcatagct ttccaccgca ctgcgcatac ggctggccat
    15961 catggaatcc tgcgcgctca tcgtactgtc agcgccactg gtgactgaat cactgcttcc
    16021 ccggttagag ccaaactggc tgagcgtgtt ccaggcactg gcaatactgc tgctgaaacc
    16081 atgcagggca ctttccgcct tattcatcga ctcccgggcc atttcctgtt gcgctgctgc
    16141 aatctgacgc gttgcattaa tgccaaccgg caaacgggac attgccccac tggcgtccat
    16201 caccatactg ccgtcacggg tctgcgtggc ggttgctcca ctaccggtct ggtgcgtcat
    16261 ctgaccaaat gacgtggtgc tgttggtgct ccagctgaag ccgttcacgt tatttgtctg
    16321 catgttgccg taggagtaat taccgtcaac cacactgcct gccgcactgg cggttggcgt
    16381 gatggaagaa gacgcaaagt gactgtatac actggagaat ccggcaccaa ggcctcgtac
    16441 catcatccat gacaacggag gtatcatggc agagatatat ccggcagtgg ccgccaggtc
    16501 agagtatttc agctgtatct gagagagttc agagagcacc accggagccc cgttctgctt
    16561 cgcatagaat gtcatggcac tgttcagaat ggcatacagc aacggccagg tctggaacca
    16621 catcagggca aacacatagc ctttcaggac ggacagcgtc agtttgttga agaccgcagc
    16681 cagcaccagg agcgggaaaa taccaatagt aatgcccacc agaatggtct gcaccatcgg
    16741 caggtttcgc atcagcacat gaccaatggc tacatgcgcc agacgctgtt tctccatcga
    16801 tgaggtggtg gccagattca ccaggctggc cgtatcaccg ttacgggcag cattactggt
    16861 gataccctct ttcagggcat tcatggtgac gttctggcgc ataatctggc tggcagactg
    16921 gctggagccg tagaaatagc tgtaactgtc actcaccagt tgtctgaata acaggtcagg
    16981 gtccggtctg ccgccaaata tctgctgcac ataataatgc caggtcttgc ctccggtatt
    17041 cgtatcaaaa ttcagcttat ccttcagtcc gacggatgcg tccttacagg acacaaacgg
    17101 ctctgagctg tgcagaaaac tgtaattatt attcggcacc tgacgtaaag ggctgggctg
    17161 agaaaagatt atggtatacg ggtcagagga ggccatcagg tcttccagcg tgtatttgtg
    17221 gttcaggtaa atatcaccca gcacgcagtt ctggacgtag tcctgaaaca gattgatgat
    17281 ttccggattc cgggacagga aatcggtgct tttcacaatc aggttcgccc cgaacagcat
    17341 gcctgtcttg ctgtaggtgg cgctgtccgg ctgagcgaaa accatctcat agccggccac
    17401 cattgcatgc ccgatacggg ttgtcagtga aagtggcatc gccagtccga ccggcacatt
    17461 atccacccgg tgaaccctaa ccaggtcact gttatcaata atctgcaccg atgtgcggac
    17521 attaaccagc agactgataa gcacaaacac ggccacccag cccagcaaat ccatcacgtt
    17581 gtgccgctgt acccacatca cggccactgc gagaacagac aatgtgagcg caatcttttc
    17641 gatggaatcc cacgtccggg tccccataaa agcggcaatg gcgttcaggt tattgcgcaa
    17701 ccactcacca ccggcaatca catagacttc attcacagcg tgcctccccc gaagtgataa
    17761 ttgttctggt aacggctgag catgcgggcg gacaactgct ggcgcatgta gctcatctga
    17821 cggtcaacaa ccagcagtgc atcctgctgg acctttacct gcccctggaa aaccgcaatc
    17881 tgttgctgtg cctggttcag attctccagc acactgtcca gcacggtctg tggatagttc
    17941 ccggtggcaa tcatggcccg ggcctgctga agcagttcct gaatgtactg gagcatgatg
    18001 tcgtagccga tatagtccgt cagctggtaa accacgctgc tggacacacc gagcatctgc
    18061 gggtcaataa gatatttaaa gacggggacg gtggtgctgg aaatgaagcc tttttccctg
    18121 tcatccagcg gagtgtcact caccgcttta ttctgaatac tggtgagcag ttttgtaatc
    18181 tgtgatttca gggctttatc ccgggcgatg gtcacatttg catctgccac caccttcaga
    18241 cacttgtccg aatcgttgca gtgataaact tttgccgttc ccccctccat aatcgcccgg
    18301 aggatatcgc ggtcagtggt acgcgccggc aggggggtaa tctcgctgtc ttccccaaaa
    18361 atcagcgtac cggtgagcgt cataatgaac tctttcagct ctttgttacc atcaagcatc
    18421 ctgtttttgg acagcgagtc ccagatgata ttgatgttct tcagcacccg ttccttatct
    18481 ttgtcactgg ccttgtcacg aacagacgat gactgtcctc cgaccgtgca gccctgacgg
    18541 gacgcggccc agtcagcaaa aatattgctc tcaccggcaa tgtcctgaca gaccttctgc
    18601 tgtgacacct gagtctgagg gaataatcct ccgacaatcc cctgtgccac ctgacaggag
    18661 ctgaggttca tgctgttaat gtcgcttgcc atcttctgca ggaaatcttt tgccgttttg
    18721 atttccggca ccgttgtctg cagggcaagg tcaaaaaagt aacccatcgc gttactcata
    18781 atctgcttaa caaaacgctg cagctgctcg ccattaataa aactgaatga gccgagatag
    18841 gcatcgatgc caccacaccc tgcattgatg tccggcagtg tcatggagat aagctggata
    18901 tttttgacct ggctgcgggc atagagggag ccaccatagg catacccggc agcctgcccc
    18961 tgccagactc cggcctgggt ggtattggtg gcaaagccca gcttgttaaa gaactggttc
    19021 atgtcgctgt tgacgtctgc aactgcagta aagggtataa agctcaggca actgcataac
    19081 aataaagctg gaatgccacc tcgatttctg cataatttat gtttaagcgt cggggataaa
    19141 aacagattca taaacggtcc tctgtcagaa aacactgcgt ttattaaaat taattaaaag
    19201 ccatgaaatt attgttaaaa aagcatctga ttacctgaac aataaagaca ttcaggataa
    19261 tttacgaaac atattcagag atatattaac aggtatatca ctgatagctc tcattacagc
    19321 atccgcaata tggacaatta aggaacacgg cattaccggt tgttttctca ttatccctca
    19381 tgttcttttt tgtcctgttg tttttatatg gtgctgtttc tgtattcagt caacatcaga
    19441 aaagttattt tcgttaaaga agaaaagaaa ccgactttgt gttgcactct gcattctgtt
    19501 ttttcagttg accacaatct acagcatatt aaatataatc atgaagtaaa taacgtggtg
    19561 taatatcccc atgagatacc ccacccactt aataccagag gaataagtaa ccagaactct
    19621 gacacagtat tccggaagcc cgttttcacc cgcccctcat gtaaatccct ctgaacccat
    19681 ccggtcagat agccactcca gaactgaaca agaaggaacg tgcctgccag tgcataagga
    19741 gaccagcgcc atgcctgcac catcataatt gctgagcact gaagaaggag agaaattata
    19801 atccccggag taacaatctg agggtttact gcataacgcc ccgacacacc cattgagaca
    19861 gaaaaaagaa caacgattaa cagtataata aaaaacaaca aaaacaatcc ggcattcctg
    19921 aatgaaagaa aaatcatacc gtcgaactga catttcaccg cagaccactc gctccagaga
    19981 agataaaatc cagaagcaac agcactcagt aaagaaatac aacttacact tcgtttaaat
    20041 atctgctgac cagccataaa ttctccctga tgttattgcg aaaccttatt tccgtacatc
    20101 tgcagaacgg tatccatccg cgccataaaa cctgcagcat ccgttgcccc ctgtaaaagc
    20161 ggtaatgcct caagcgtatt gacgttaacc aggaaggtgg tcggtgtggc caccgggata
    20221 ttcgggaaga atgttttcag tacatccggt ggtgcaggta acgcctgcgg gaaggccgta
    20281 tcaccctgac catccagggt atagggaaat acggaaaatc cgtactgcag cgccagttgt
    20341 ttcagtaccg ggtcaaactg gtgacagtac ggacatcgcc cctgcatgaa cagcaccact
    20401 ttccagtcag caagattgac ctgagttccg ttgctgaggc ggaaccagcg ggttgctgtt
    20461 ttctcctgtc gcgcctgtgc atctgctgcc ggctgagttg tccgtgctgc cattccctcc
    20521 ggatgccaga gtcgctcgat ttccgcacgt gtggatgcct gtgtacagct ggccagaaaa
    20581 agcaggacaa cggacagcga tttaacggga aacatgttca tcatcattat ccttccgttc
    20641 tctcagtaac aactgctgtc tgacttccag tgcctcacgc tcttcctgac tgacacgggc
    20701 tatccacgta tcaagcacac gatatgctcc ccacaggacc agaacagccg aaataacctg
    20761 agccaggata tatgccatcc agggcttgct gttaaccaga cgggcaacaa tatggaacca
    20821 gacacccagg gaaaataccc acatccccgt aatatccagc cggggtaaag agaatctttg
    20881 tttacttatc atttgaatat cctgttgtgg tttacatgaa tttattcacg catataatct
    20941 gcttaacttt cgtgaaatta atcagtgtgt tatatgaaaa agttatcttt caaagaaaaa
    21001 ctggaaatag tgagaaacat cataagagag tcattatcag gtaatgcggc ttttgcttta
    21061 ctggtgtatt cattattgca ttccttcccc attaaaacct ttgctgattt atttgtgacg
    21121 atactgttat ttatagcaat tatcgctgtt gtgatatggc tatttctcat catctatgtt
    21181 tatttttgtg aattattcag aagtcactgg atagcagtct ggtttatcct ctggtctgtg
    21241 gtaatcaacc tcactattgg atttgtcatt tatgacaaat tagcaccagt catttcacta
    21301 atgaaataat aaaaaaccac attactcctg accacgctga tgacaacata atacagacaa
    21361 atattcttct gaacctgtcg aacttcgtga taaccggcat ggtaccgact gccattgaaa
    21421 caacgggcac ggcacactga ttcataaccg caccacaaag aaaccagaat ctggcagtca
    21481 gcacatataa acacaaaata ttaataacaa gaaaaaccag cgaactaagg aaaaatatct
    21541 ttctgtcatt acgctcagaa aagaaataac caacaataat gatgaatatt aacaaaacaa
    21601 tacctgtaac aatatttaaa ctgtcttcac tcataaatta aacccgcgtt ctaaaattcg
    21661 gtttaaaatc ttcagatacg ttcagaaact gtttagccag gtcgccctgt gaaaatttgt
    21721 aacatttatt ctctggttat atctctttct cgcattggcc attcattatt tcattatggt
    21781 ttctgatggc tggagtaacc agtagaatat aatcattccc gcaccagaaa acactgcggc
    21841 aacagcgagc cacaaacgct tatgcactcc ttctgatatg cctgaaaaat atcctgtatg
    21901 gagtagcagc agcaaccatg acaacactcc tgccagaccc ggcagggcag gagggatatg
    21961 ccacagttca tatttcataa taacaatacc ttctgccatc atcatggaca gggtacaaac
    22021 aacaaaacag tcccatgaaa aaaaacgcct gtcagcagca aggaaaatca ctgtcagtga
    22081 catcactgtc acctgggcca gcaataaagg ccataatgaa ataaaattca tttaaacgac
    22141 ctctttaaaa attcggttta aaatcttcag atacgttcag gaactgtttt gccaggtcat
    22201 cctgcgaaat aaaaccgtat gataacgggc gaacactgcc ctgtttcggg tctaccagca
    22261 tcatggccgg gaaatatttc acgccgaggc gctgcgcctg cccctggtca gtccgggaat
    22321 ccggcaacag cggattaatc acgccatcca cggaaacggg aataacactc agaccatacg
    22381 tatcccggaa gccattaatg acctgcgcca gctgcccgtc gatggggtcc tgcccccggt
    22441 aaaaaaacat gatgccgtag tgttcagcca gtttcgcaat agcctgtcgc tgctgcgcct
    22501 ggtctgctgc cagctggttc cgtaccgtgc cgttgtaatg gctgtactgc aggttatagt
    22561 ccagttcagg atgtgccagc atggcctttc tggcgctcat ggtgaaaagc ccggcctgct
    22621 gagtccagta attctgcagc cggaaatatt tcacaaaatt atccacgccg ggatacagaa
    22681 tggcttcgta cagcgcccgc tttgttgccg tctgcagcgc ggtcagtttc tgcataatat
    22741 ccggctcctg acgaggagcg gctggtactg gtttattttc tttttccttc ggatttattt
    22801 tctcgttata ccactgccag cctgcatctt tccctgacgc cggaaaaata aagcagcaga
    22861 gtagcagtgg cagtaatgct ttattcatct tcattccttt catcactttt tctttcatca
    22921 gctttctgca ggtcagtcag ggcagaaaag caaaaataca gaataaacag gagcacaccg
    22981 gagtaaagga taacgaaagc agcgacaagc accccgcctg ccgtggtttc ttttaccgta
    23041 tcccatgacc agataattac cggggaaatg acaattgctg taataacaag ccggataaga
    23101 aaatcaacaa gacgattcga cgtgaatatg actttcatta ttttcccgac cttactgccc
    23161 cacctgtttc agctgaccgg caatctgctc tttcactttt tcggtcagcg caccattatc
    23221 cgggattttc tggttattca tcaggtcttc gtagaagtta gtgaagtcca gctggtcgaa
    23281 cttaatacgc tgcagttcat ccacggttat tccccggcag tcaggacttt ttgcgctgcc
    23341 aaacccgata cgcaactgcc cgttacgccc ctgctgctgg actatctgcg ccagcttcga
    23401 atcaaactgg cagtaactgc gctttttctg cagacagaca cccagcactt tccgggagca
    23461 gaactcgccc acactcaccg tcagtttgtt gtcctttgcc tttcccagcg ccttttcttc
    23521 gctgttgcac ttcgccagac ccacatcctg accccagcca ccatccttac agcagttact
    23581 gaagccggct gcggccttac ggcagaattt cgcctcgccc gtgaaagccc tgacatccac
    23641 accattcagg gcggcaacat ccttacctgc ggcggcaagc gcagccagct gtgatactgc
    23701 ctcaccaaaa tcactgtttt ttccgctctg cgccttatcg cattcaccgt caaggcagaa
    23761 gacatcacca ccacagacca tcacttttcc tgatgttctg gactcgcagg agtacgtggc
    23821 atactcatgc agacagcttc cctcatcgga ataaaacgcg cactgacgtg agaccagcgt
    23881 acaggccgga ttatcgacat atttctgaca ggtgccatta tctgccgact gcgtgacata
    23941 tttatcccgg tatgcccagc atgcctgagt gatgctccag ggcttaccct ccgtcacacc
    24001 ggttttcgtc tctcccggct ctgaacactc agtgcctttc agcacgccat ccgctttatt
    24061 aaacggacaa ctctccaccc actccacccg tggaacccat tccttatcac ggaccttcat
    24121 cctgagtttc agcgtaaagg tggaagcacc actgacaagc gattcataga ccatcctgtc
    24181 accattcccg tgcgggaggc aattaccgtt tgcagtacag ccactaccgg tcagaacctg
    24241 cccctgtgtg acagaaaacc cggagggcac aggtatggtg aaagtcccgc tctgtcctgt
    24301 aaccagttgc acattaaagg ctgtgttcat aaagtcgtaa cgggagttaa ggaaatatag
    24361 ccctgcatga gccgacagcg aggcactttc cactgtcccg gcaacaggta cggtaacgct
    24421 gaacaccagt ttcagaccgt tcatactgaa ccggaactgt gagtgcggta tcgtcacctc
    24481 acgatattca tccgtgtatt tccaggctcc tgtgatcctg gctgtcctgg aacaatattc
    24541 ctccaccatc gtgtcacgtt cacaggtata gtttgtgaat tcactgcggt ttatctgctg
    24601 cgcactgcac tgctgtccgg tatttcccac gatactgtca gcccggttca ccacatccct
    24661 gccggtctga ataaacggcg catccggtga cagaatgtct ttcggcttgt tcatgaatga
    24721 ctctgtgatg gttttgccgg tttcactggt tgcccattgt gtggtgccgt tatttttcag
    24781 accgccatcc ccgccggcag tcaccccacc gtaatactgc gtctcatccg ggttcgcgtt
    24841 atacccgggg atactctcct gcggattaaa actctgaata ctgccaatac cctgcccctg
    24901 gatctgacgg gcaaaatcgg aacctgcccg gtaatcactg ttgctgtcgg caaatacaga
    24961 aaagctcagg aaaaaaacaa ttgcacaaat attgcgcggt cttccggagt cattaacatt
    25021 cagagacctg aaaaaggtat ttatcttttc tttcataacc tgatccatac tggagaatga
    25081 agtgatgtca tctcaacttc agaacggtgt tgattttggt gtatggagct tcctggcgta
    25141 ccgggattac tactcagata acgacttcag taacaatatc agcgtcctga agtctgcacg
    25201 cagtgaacga cgacagtacc ggtgatacca gcaatgcgga ccttccggct gaagccggaa
    25261 acagagctga aggtcactgt taaaagcccc cttcaccgac catccccatg tcagacgtgg
    25321 tattgctgat tcagcatcct tccgcagtcg ctgctcaaga agggcagcca tttttaccgg
    25381 acaggcaaag gcaatataag gcagcagctt tgtgaccggg aaaccatgtc cctgacagga
    25441 ggcatatgta cggaatccgt gaacgttcat cgcggccacc agtgcacgga tgtccgggtc
    25501 aatccggtaa tacccggcag gtaaactcat tgcggcttat ccccggtgcc gtccagcagt
    25561 ccctgagcca cctgccggca gtctcccgtg gcggccactt tttccagcgc ctgcctgacg
    25621 cgcagatttc cccggatgat gtcgtgcccc tggctgcaga acaccgccag ggccggcacg
    25681 ctgcggatac cgtactgtga aaacaacgtc gggtcgatct gaacaccatc ggtcgcgcca
    25741 tcttttacca gagacaacac ggcttcagcg gtagtcttca ggtcattgtt caccatgccc
    25801 cgcagtgtgg ccggaatacc gtagtgccgg gtttctccca gcatccgttt cagtccctct
    25861 tcgggaatgg agaacgatac aaaatacacg gccccctgtt gtggtttgtc tttctgagca
    25921 gcctgctgtt tacggaccag ttcatcgaga aactggttat cggcatgctg aaggggattc
    25981 gccctgacct gctgttctgc ccatgcctgc agttgtgtgt ccggcttttc acgcaactgc
    26041 tgactcagtg tttcctgctg cttcaggaac tggcggtttt cgggcgtatt cacgttttcc
    26101 gaagcatggg cagaaagaaa caatcctgct gtcagggcga ataaaatgta tatttttttg
    26161 ttcatagaat tctcctgcaa acagacagta attaatcgtc ataatgaggc gctcaataga
    26221 gcgagtctgg agatagctga cgccatgtta aacacaacga agatcgttga ttaacttctt
    26281 tctccattta tcttttttct gagactggca gcaattagat aaacgatacc ggatattcac
    26341 tctgttaaat cattaattca tttctgaccg atgcacataa aatgagtcag atattacccg
    26401 ttattctctt cttcattttt gtttagtctc tttcccggca gaaactcatc agagcagaga
    26461 cattccaaaa aaacagccgc ccaggtgcat aaaaaagttg ttcccagata tagcctgata
    26521 actctgatcc tgtctgacac atcacttctc tgccattcac tgataatgtc actgataaca
    26581 ttttcaggca taaaataaaa caacaccgtg aatagcatca ccagtgcaaa aggaaacaat
    26641 tttaatgctt tgcctgtcaa acgaatgaac acacaggata tactcatata aatatgaatg
    26701 tatttactgc ttaatttctt ttccatttaa aatattcctc tttttgttta agttacagaa
    26761 atacacagtt acgtttacgc cacatcaggt aaccgaagtt tttctttgtg ttgggcggat
    26821 tttttcccgt ctcccagcgc atcacactgc gtccgaacgg atggcactgt ccgctgtccg
    26881 ggtacatatt caccatctga taacgccagc gctctttggg aagtatgggc gacggatatt
    26941 cataacagac agccacatcc acgccgatgc tgttcattat cattccctga cggtggagct
    27001 taaacgccat ccgttcactg accagcaggg aggactgcaa cggactggat tcattgctca
    27061 cccagccatt aaacggatac atactgccct gcgaaccggc acaccagaac aggatatcca
    27121 gcggcttgtt aaaggcactg gcaatggcat ccgccgcaca ggctccctgt gctatcggat
    27181 ttgcaaagat gaccgcttcc gggttgagaa tggtggtcag actgctgtct gtccaggtag
    27241 ggtcaatttc tgagatgtac ccgatatcca tatcgccgcc catcagacat cctgctgagg
    27301 tgataatact cagccagtat gtcagcgggt atttgtacca gtgaacatgg taaaatgccc
    27361 cgttcacctg cttgtcgtct tttttggccg ttccctgccc ggttttcccc agctccggga
    27421 ttgttattcc aagatttacc atacatcccg gactgcgggt cacatccgtc agtgccatcg
    27481 gctcccagta accaatagcc agcccgatac gtttaaatac aggcggtggc gcaggacaga
    27541 actggatcgg cattgacggg tttattgtat ccggcacctt cccctgactc acttttgtgc
    27601 tgcccagcga cagtggaaaa atgcagttcc agcagatatc cgtgatcggg ttaacaaacc
    27661 gcccttcaca ggcagcgtcc gctgacgctg cgggaacatg accagtcagc agcaaaacca
    27721 cacacaacca catacaccgt ttcatttccc tccctccccg accggaataa actccacctt
    27781 caggaaacga tcgccactga cagcactcac ccgtgccggc acctgatcaa tgccgagacg
    27841 ctggcagatc acgccgttct gatcaaagta aacacgactg tccagagcct tctgcatctc
    27901 cgggatactg ccctgcacca ggataatttt gctttccagt gtgggaggta tctggcgttt
    27961 catccattca atctgctcag gatcatcacc gttgatgaaa tacagtgtct ggttaaaagg
    28021 cacatactgc agcgggttca tcacctcccc ctggcgggcg aagatacgac cttcgttatc
    28081 ccggatatcc gcagccagtc tgaccgacgg gtcaaacagg cggctgctgt atttctccgt
    28141 ccgactgata ccgggcacgg cagggggccg caggctgttg cggatcaccc gctccttaaa
    28201 cgcatccatt ttccggccca tctctccgga ctgctccaga gctgtcagac gctgcataat
    28261 caccgtcagc atgtccggct cctttaccgg ccacaaatcc ccccaggtgc caagatcagc
    28321 tgcagccaca ctctgccccc ataccagcag ggcaatcagc ccccggcatc tcatggttct
    28381 gccctcattc gccgagcaat atcctgctgg atttcacggg tgatatccgg tgccccctgt
    28441 accacggcag gcgacaccag aatgacagcg tggtgcttct gctgccacgt ctgcagactg
    28501 gcctccagtg ccgcgttaaa acggtcagcc agcgccttcg actgcgcttc tgacagtttt
    28561 ttctggctgg cgctgtcaaa aaacgcatcc actgtctgtt tcatgttgaa ggcgacggtt
    28621 accggcgtgt tcagtctgac aatgccgtaa ctgacggctg cgttcagtaa caccattgcc
    28681 aggcatcccg gcaccgtcca ccaccagtgg ctgcgtcgac gggctgtcac atcagtcgtt
    28741 ttttgcgttg tggtcatgca acactcctgt atttctcatg ttcttccagc caggcctcca
    28801 gcgaagccat ctccgggcct gacttcttcc acgccagctg ccacaccgcc tcatgaatgc
    28861 tcagcccctc tttacgtttc tgctgcacaa actcaaaatc cgggccgtca gagctgtaca
    28921 tggcacggct tagcgggtcc acaaacagac ggtgccagga ggaatggttt tccacctgca
    28981 gcaggaagga actgaaccac tggtctttgg ccgcaccaaa cttgccaatc atgtcacgct
    29041 gcagtggcag gaactggtcc gggaataact ggttatattt cgcgaactcc ttcgcactct
    29101 gcttgaggat aattttgtag gaggagttac cccatgcagc acgggccgca ctggaggcct
    29161 tgtcagagtc aaagtcgacg atgttctgtg taatggtgat ataggcaccg gtatgacggc
    29221 gggcagtacg gtagcctttt tgaataaact caccgacttt acgatttttg aagtccagca
    29281 ggcgccagcc ttcatcaatg acgttcagtt ttttgagagt acgcggcgtg cggtacatcc
    29341 tgttctcgat atagataatc agggagaaca tcaccgcaac cagcagtgac ggacggtctt
    29401 ccagtccacc cagctccaga acaaccattt tcgcatcatc ccgcagggac ggcttatcag
    29461 agttaaaata ccggccgtag gtgccgttgg cagtgtactg gtcaagcagc acaatcattt
    29521 catccaggcg gctgcggatg gtcggtgact cggcatactg ctcactgtca ctggcgtttt
    29581 tcaggaaatc caccacgtca tcaatacgtg ccctgttctc tttggccagc catgatgccc
    29641 tgaccgcctg cagcagcaga ccttcatgca cttcatccag gttaccgttg gggctggcca
    29701 tcaccgacag ctggtcacgg atacgctccg ctgactggtc aatatcggtg atgttagcaa
    29761 acgggttaaa gcgcagggtt tcaccgtcca ggtatacgcc ccccatgttt tcacacaggg
    29821 acttgtagcc atcccccatg tcgaacacca cggcaaagcc cccggagtcc agcacgctgc
    29881 ggataagtgg ctgtatcagc ccggttttac cggccccgga agtgccacag accgccatgt
    29941 tgtagttggt gttattcatc cccttaaaga agatatcgat aaacgccagc tggttacggt
    30001 aggtgggtgc cagcagacct gccggtgtca gggggttatc tgccactaac ggcatcaggt
    30061 tgaccacatt aaagctctct gcacgctgta ccacgccggc ctctttcagc tgtctgaaca
    30121 gccctttccc ggccataaag ggcaggcagg tcaggaaatt gcgcatgtgg ttaaagcgcg
    30181 gtgaaatcag ctcaaaaccg tttttacgga agctgttcag gatgtcctgc tccacttcca
    30241 gcgccgtttc attgttgtcc ttgcagaacg ctgtgatgtt gaggaagtag gacaccacgg
    30301 aggactgacc tgagcccagc cgctgacgca gttcccccca ctccttcgct tctttctcca
    30361 cggacggaaa ccatttggca taggaggtct tcgacttctt ctccaggtcc atgtacttca
    30421 ggttggcttc gctgtgggtt ttcacctggt cttccaccac cagggtcagc gtcaggatga
    30481 acgggcagga aatggacatt tccgggttca gcaggttgct gtagttgtcg gccatgttcc
    30541 acaggaaggc gatttccggg ttacgggcca gatggaaatt cagaatacgg gccgtactgt
    30601 tcctgccgtt ctcacgcagc cccagcgtca ggtaatccgc ccggaccttg aggtcaaaac
    30661 tgtcctccac acactgataa ttcagatcag aatacgggtc cagatgacgt cttttcgggt
    30721 acagggagtc cggattatgg ttaatcatct ccccgacaat atcgataaag gcctgtgcat
    30781 ccactgtctg tgtggtgatt tttgcgccgt gaaaagaggc acgaatgatt ttcaccagat
    30841 tttccatttc cagaatgtct gcccggcttt ttttctttga aggagaacag tacgagatga
    30901 acacccggta gtgacgcagg gtcagcggca gattcatccc ctccggcagc ggaaacagtg
    30961 tttctgcggc tttcatgtaa taggcccggg taatggcgtt aaaccgttct gcctgttcac
    31021 ctgaccagga gaactcacgc agcccgtatt caatcctgtc accaaccagc tgactggaca
    31081 tcagatggat acacagcgga ataccgcgcg gtagtttggt gcgcagcata tgatcgagcg
    31141 cctccacaat ggtctcattc gcaccattga tgggaatagc ttccagcata aagcccattg
    31201 tggagtcatt catgaacaga ccggattcct ggttgtaatc acggtatggc agcagacggc
    31261 tgaactgcgg gaagttcatc tcaccgagga tctgattagc ctgagcagat tcatccggta
    31321 atttaagtgc ggtcagcagt gaattgaccg cctgagtgac gacctcaagt gggttattac
    31381 tcatatgagt attccttagt acgttttaaa aaggaaaata tcagtctgtg gcgtggatat
    31441 atcacgcagt gccattctta aaaccacagc acaaataaga gatggaggct tttaacagga
    31501 aaccaggatt acaattaacg ttcctttgct gaatcagcat cacttaatgc ctgaagttgt
    31561 ttttcgcgta ttttattgcg ttcattttct cttatccgtc gcgcctcttc catttcattt
    31621 ctttttctgc attctgatgc aattttattt gccttaccaa gggtta
//
LOCUS       DAZKHK010000052        31575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKHK010000052 DAZKHK010000000
VERSION     DAZKHK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.052"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            107..2335
                     /gene="crfC"
                     /locus_tag="ACHOS5_002363"
     CDS             107..2335
                     /gene="crfC"
                     /locus_tag="ACHOS5_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP9777176.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKIPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISQLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSTNHFLRQQQDAVNAQLAALFQD
                     DSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQAATDEQSTRLGVVREIFSRWLNQPD
                     WGRGNEKFSTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2332..3210
                     /gene="rdcB"
                     /locus_tag="ACHOS5_002364"
     CDS             2332..3210
                     /gene="rdcB"
                     /locus_tag="ACHOS5_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP9777177.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            3474..4976
                     /gene="proP"
                     /locus_tag="ACHOS5_002365"
     CDS             3474..4976
                     /gene="proP"
                     /locus_tag="ACHOS5_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP9777178.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5088..5177
                     /gene="pmrR"
                     /locus_tag="ACHOS5_002366"
     CDS             5088..5177
                     /gene="pmrR"
                     /locus_tag="ACHOS5_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP9777179.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5153..6253)
                     /gene="pmrB"
                     /locus_tag="ACHOS5_002367"
     CDS             complement(5153..6253)
                     /gene="pmrB"
                     /locus_tag="ACHOS5_002367"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP9777180.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(6254..6922)
                     /gene="basR"
                     /locus_tag="ACHOS5_002368"
     CDS             complement(6254..6922)
                     /gene="basR"
                     /locus_tag="ACHOS5_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP9777181.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6919..8562)
                     /gene="eptA"
                     /locus_tag="ACHOS5_002369"
     CDS             complement(6919..8562)
                     /gene="eptA"
                     /locus_tag="ACHOS5_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP9777182.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNITALNLPGQCINGECYDEVLFHGLEEYINNLQSDGLIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8666..10003)
                     /gene="adiC"
                     /locus_tag="ACHOS5_002370"
     CDS             complement(8666..10003)
                     /gene="adiC"
                     /locus_tag="ACHOS5_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP9777183.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10140..10901)
                     /gene="adiY"
                     /locus_tag="ACHOS5_002371"
     CDS             complement(10140..10901)
                     /gene="adiY"
                     /locus_tag="ACHOS5_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP9777184.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKESISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11237..13504)
                     /gene="adiA"
                     /locus_tag="ACHOS5_002372"
     CDS             complement(11237..13504)
                     /gene="adiA"
                     /locus_tag="ACHOS5_002372"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP9777185.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13703..14635)
                     /gene="melR"
                     /locus_tag="ACHOS5_002373"
     CDS             complement(13703..14635)
                     /gene="melR"
                     /locus_tag="ACHOS5_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP9777186.1"
                     /translation="MFQERESSMNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPH
                     IMPTSHWHGQVEVNVPFDGDVEYLINNEKVSINQGHITLFWACTPHQLTDTGTCQSMA
                     IFNLPMHLFLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQL
                     AIDEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTI
                     NDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGF
                     RSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14894..16249
                     /gene="melA"
                     /locus_tag="ACHOS5_002374"
     CDS             14894..16249
                     /gene="melA"
                     /locus_tag="ACHOS5_002374"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9777187.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(16246..16896)
                     /gene="yjdF"
                     /locus_tag="ACHOS5_002375"
     CDS             complement(16246..16896)
                     /gene="yjdF"
                     /locus_tag="ACHOS5_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP9777188.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(17019..18665)
                     /gene="fumB"
                     /locus_tag="ACHOS5_002376"
     CDS             complement(17019..18665)
                     /gene="fumB"
                     /locus_tag="ACHOS5_002376"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9777189.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(18743..20083)
                     /gene="dcuB"
                     /locus_tag="ACHOS5_002377"
     CDS             complement(18743..20083)
                     /gene="dcuB"
                     /locus_tag="ACHOS5_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP9777190.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(20654..21373)
                     /gene="dcuR"
                     /locus_tag="ACHOS5_002378"
     CDS             complement(20654..21373)
                     /gene="dcuR"
                     /locus_tag="ACHOS5_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP9777191.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDSPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(21370..23001)
                     /gene="dcuS"
                     /locus_tag="ACHOS5_002379"
     CDS             complement(21370..23001)
                     /gene="dcuS"
                     /locus_tag="ACHOS5_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9777192.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRTTDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            23182..23412
                     /gene="yjdI"
                     /locus_tag="ACHOS5_002380"
     CDS             23182..23412
                     /gene="yjdI"
                     /locus_tag="ACHOS5_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP9777193.1"
                     /translation="MDRALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            23424..23696
                     /gene="yjdJ"
                     /locus_tag="ACHOS5_002381"
     CDS             23424..23696
                     /gene="yjdJ"
                     /locus_tag="ACHOS5_002381"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9777194.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            23925..24221
                     /gene="ghoS"
                     /locus_tag="ACHOS5_002382"
     CDS             23925..24221
                     /gene="ghoS"
                     /locus_tag="ACHOS5_002382"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9777195.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            24249..24422
                     /gene="ghoT"
                     /locus_tag="ACHOS5_002383"
     CDS             24249..24422
                     /gene="ghoT"
                     /locus_tag="ACHOS5_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP9777196.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(24541..26058)
                     /gene="lysS"
                     /locus_tag="ACHOS5_002384"
     CDS             complement(24541..26058)
                     /gene="lysS"
                     /locus_tag="ACHOS5_002384"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9777197.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMTDLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(26295..27752)
                     /gene="dtpC"
                     /locus_tag="ACHOS5_002385"
     CDS             complement(26295..27752)
                     /gene="dtpC"
                     /locus_tag="ACHOS5_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP9777198.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(27811..29958)
                     /gene="cadA"
                     /locus_tag="ACHOS5_002386"
     CDS             complement(27811..29958)
                     /gene="cadA"
                     /locus_tag="ACHOS5_002386"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP9777199.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(30038..31372)
                     /gene="cadB"
                     /locus_tag="ACHOS5_002387"
     CDS             complement(30038..31372)
                     /gene="cadB"
                     /locus_tag="ACHOS5_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP9777200.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         7970 a         7617 c         8020 g         7968 t
ORIGIN      
        1 taaatataaa attatttcct gcagtaatta taaaagccga aaacagaact caaaaacaat
       61 ctggctaaat aaaagtaata aaatttgctt taaggaagaa tattctatgt acacacagac
      121 cctgtatgag ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca
      181 gttactggaa aaaatacctc tctctgtacc cggagacgat gcgccacaac tggctttacc
      241 ctggagtcag cctaatatcg ccgaacgtca cgcgatgctg aataatgagt tgcgtaaaat
      301 ttcccagctg gaaatggtgc tggcgattgt cggtaccatg aaagcaggga aatcaaccac
      361 cattaatgcc attgttggta cggaggttct gcctaatcgc aatcgcccaa tgactgcgct
      421 gccaacgctt attcgccata cgcccgggca aaaagaaccg gtactgcatt tttcacatgt
      481 cgcgccaatc gaccatttaa ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa
      541 gcatctgacc gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggattga
      601 aaatggcatc gctttcgaaa aatattatct gggtgctcaa cctatttttc attgtctgaa
      661 aagtttgaat gatttggtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc
      721 ttacgccgcc attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct
      781 ggagagttat cccggtcagt tgacgttact ggataccccc gggccaaatg aagccgggca
      841 accgcatctg caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt
      901 gctggattat acgcaactga aatcgatctc cgatgaagag gtccgtgagg caattttggc
      961 ggtggggcaa tcggtgccgt tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa
     1021 cagtgacgac gccgaccagg tgcgggcact gatttccgga acgctgatga aaggctgtat
     1081 tacgccacag cagatatttc cggtgtcgtc gatgtggggc tacctggcga atcgggcgcg
     1141 ccatgagtta gccaacaacg gtaagttacc accgccagag caacaacgct gggtggaaga
     1201 ttttgcccat gccgcgctcg gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca
     1261 tattcgccat gctgccgatc agttgtggga agattcgctg ttcgcccagc caattcaggc
     1321 gttgcttcat gccgcttacg ctaacgcctc gttgtatgct ctgcgatctg ccgcgcataa
     1381 actgttgaat tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa
     1441 cgtcgcttgt gaacaattgc ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt
     1501 gcaactcaat caggcgcagg tgagcggcga gattaaacat gaaatcgagt tggccctgac
     1561 ctccaccaac cactttctgc gtcaacagca agatgcggtg aatgcgcagt tagccgcgtt
     1621 gtttcaggat gattcggagt cgttaagtga gatgcgtacc cgctgtgaga cactgttaca
     1681 gacggcgcag aacaccatca gtcgcgactt tacgctgcgc tttgccgagc ttgagtccac
     1741 cctttgccgg gtgttaaccg atgttattcg ccccattgag caacaagtca aaatggaatt
     1801 gagtgagtct gggtttcgtc ctgggtttca ttttcctgtt tttcacggcg tagttcccca
     1861 cttcaacacc cgccagctgt tcagtgaagt catttcgcgc caggcagcaa cggacgagca
     1921 gagcacgcgt ttaggcgttg tgcgtgagat tttttcgcgc tggttgaatc agcccgactg
     1981 gggacgggga aatgagaaat tctcgacaga gacggttgat tacagtgtgt tgcagcgagc
     2041 attaagcgca gaagtcgatc tttattgcca acaaatggct aaagttctgg cagagcaggt
     2101 cgatgaatct gttacggcag gcatgaatac ttttttcgct gagttcgctt catgtttgac
     2161 ggaattacag acgcgtttac gcgaaagtct ggctctgcgt caacaaaatg aatcggtggt
     2221 caggctgatg caacagcaat tgcagcagac tgtgatgact cacggctgga tttacaccga
     2281 cgctcagctg ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag
     2341 acgttactgg acggccccgg tcgcgtgctg gagtcggttc atccgcgctt tttagtggat
     2401 ctggcgcagg gcgatgatgc ccgccttcca caagcccatc agcagcagtt tcgtgaacga
     2461 ctgatgcagg aacttcttgc gcgcgtgcag cttcagacat ggacgaacgg cggcatgtta
     2521 aatgcgccgc ttagtctgcg tctgacattg gtggagaaac tggcgtcgat gctggatccc
     2581 ggtcatctgg cgctgacgca gatcgcgcag catctggcgc tgctgcaaaa aatggatcac
     2641 cgccagcact ctgctttccc ggagctcccc cagcaaattg tcgccttgta tgagtggttt
     2701 tcagcccgtt gtcgctggaa ggaaaaggcg ttaacgcaac gaggcctatt ggtgcaggcg
     2761 ggtgaacaga gcgagcaaat ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg
     2821 ttgcctgggc gctgctttat cgttctggag gagttgcgct ggggtgcatt tggcgatgcc
     2881 tgccgtctgg gaagcccgca agcggtggcg ttgttgctgg gtgatttgcg cgtgaaagcg
     2941 acacaacatt tggcagagag tatcaatgcg gccccgacca cgcgtcacta ttaccatcag
     3001 tggtttgcct cttcgaccgt ttcgacgggc ggggatcatg ctgatttttt aagttggctg
     3061 ggaaagtggt ctacggcaga taaacaaccc gtttgctggt cagtgaccca acgctggcga
     3121 actgtcgcac tggggatgcc acgactctgt tcagcgcagc gtctggcggg ggcaatggtc
     3181 gaggaaatct tctctgtaaa tttggtgtaa ataatcagtt acaccaatga gtcctaaacg
     3241 aaatgcatgt gtgaagttga tcacaaattt aaacactggt agggtaaaaa ggtcattaac
     3301 tgcccaattc aggcgtcaac tggtttgatt gtacattcct taaccggagg gtgtaagcaa
     3361 acccgctacg cttgttacag agattgcatc ctgcaattcc cgctcccctt ttgcggccgt
     3421 cgcgctgatt tttctggcgt ttgcggaaat gggccaactc tgcgaggaaa gctatgctga
     3481 aaaggaaaaa agtaaaaccg attacccttc gtgatgtcac cattattgat gacggtaaac
     3541 tgcgtaaagc cattaccgca gcatcactgg gtaatgcaat ggaatggttc gattttggtg
     3601 tttatggttt tgttgcttac gcattaggta aagttttttt cccgggggct gaccccagcg
     3661 tgcagatggt tgctgcactc gccactttct ccgttccctt tctgattcga ccgcttggcg
     3721 ggctcttctt tggtatgttg ggcgataaat atggtcgcca gaagatcctc gctatcacta
     3781 ttgtgattat gtcgatcagt acgttctgta ttggcttaat accgtcctac gacacgattg
     3841 gtatttgggc accgattctg ctgttgatct gtaagatggc acaaggtttc tcggtcggcg
     3901 gtgaatatac cggggcgtca atatttgttg cggaatactc ccctgaccgt aaacgtggct
     3961 ttatgggcag ctggctggac tttggttcta ttgccgggtt tgtgctgggt gctggcgtag
     4021 tggtgttaat ttcgaccatt gtcggcgaag agaacttcct cgattggggc tggcgtattc
     4081 cgttctttat cgctctgccg ttagggatta tcgggcttta cctgcgccat gcgctggaag
     4141 agactccggc gttccagcag catgtcgata aactggaaca gggcgaccgc gaaggtttgc
     4201 aggatggccc gaaagtctcg tttaaagaga ttgccaccaa acactggcgc agcctgttga
     4261 catgtattgg tctggtaatt gccaccaacg tgacttacta catgttgctg acctatatgc
     4321 cgagttattt gtcgcataac ctgcattact ccgaagacca cggggtgctg attattatcg
     4381 ccattatgat cggtatgctg tttgttcaac cggtgatggg cttgctgagt gaccgttttg
     4441 gccgtcgtcc gtttgtgctt cttggtagtg ttgcattgtt tgtgttggcg atcccggcgt
     4501 ttattctgat taacagtaac gtcatcggcc tgatttttgc cggcttattg atgctggcgg
     4561 tgatccttaa ctgctttacg ggcgttatgg cttctacctt gcctgcgatg ttcccgacgc
     4621 atatccgtta cagcgcgctg gcggcggcat ttaatatttc ggtgctggtt gccggtctga
     4681 cgccaacgct ggcggcctgg ctggtcgaaa gctcgcagaa tctgatgatg cctgcctatt
     4741 acctgatggt agtggcggtg gttggtttaa tcaccggcgt aaccatgaaa gagacggcga
     4801 atcgaccgtt gaaaggtgcg acaccagcgg cgtcagatat acaggaagcg aaggaaattc
     4861 tcgtcgagca ttacgataat atcgagcaga aaatcgatga tattgaccac gagattgccg
     4921 atttgcaggc gaaacgtacc cgcctggtgc agcaacatcc gcgaattgat gaataagctg
     4981 aaacggatgg cctgatgtga cgctgtctaa tcaggtcaat tgaactctta aggttcactt
     5041 aatctctgac gcgcatactc tcctccaggt taacggagga gagtgcaatg aaaaaccgtg
     5101 tttatgaaag tttaactacc gtgttcagcg tgctggtggt cagcagcttt ctttatatct
     5161 ggtttgccac gtactgatct tttttcagcc gtatccaggc ccgcgtgccg gaagtctctt
     5221 gccggttttg caggaaaaac tgcccgtgat gcaactgtgt aatgcggctg acaatactta
     5281 accccagacc aattccgcca taacggctgt ccattcgtac aaacgcttta ctcaactccc
     5341 cgcatttact ctcatcaata cccggccctt catcttcaac ggccatgacc gctccgccat
     5401 cttcttgcag cttaatcata atgttgctgc cttgtgggct gtaacgatgg gcgttttcta
     5461 ccaggtttcg caataacatc cgcagcaggg ttgcatcacc ctgaacggtg atgtcggcgg
     5521 cgctctctgg caatagcagg gtttgctggc gctggtcgag catggtactg agttcgtcat
     5581 acgaggggag aatgacatct tccagcagtt ttacatgttg ataattaccg gaagaaaatg
     5641 actgtccggc acgcgccagt tgcagcagct gggagacgct ctccatcatc tgatcaagtc
     5701 gtgccactaa cggtgctaca tcaatgtgat gcgttttcgc cagcagttcc agatgcaaac
     5761 gcacccccgc cagtggcgtt cgcagttcgt gcgcgacgtc agcggtaaac aacctttcgt
     5821 tatccagcgt gctggtcagg cgactgacca gatcgtttaa cgccgaaacc accgcatcga
     5881 tttcgagggt ggcgctatga atggcaatgg gcgttaagtt gtcggcggtg cgcgcttcca
     5941 gctctttttg cagctccgcc agcggtcggg tgatgcggcg taccgcctga tagcagataa
     6001 atagcgtcag gctaaccata aagacgccgg ggacaatcag gctggcgacc gcctcgcgga
     6061 tctcacgcat gatgtggcga tcgttgttgc gattgtcgcg tagcgcctgc tcaaacagct
     6121 gaatctgctc ggtactttca tgccatagcc agaagacgct gatcagctca aacaccaaca
     6181 aaatggcccc gatggtcaat atcagccgtt ggcgcagcga tattggtcgg cgcagaaaac
     6241 gcatcagatt caattagttt tcctcatttg cgaccagcat atagccaaat ccgcgcacgg
     6301 tgcggatacg ggctttgccc actttgtcgc gcaggttatg gatatgcact tccagggtgt
     6361 tggtcgaggg ctcgttgtcc cagttgtaga tatcgttgta gagaatttcc cgatgcaccg
     6421 ggctgcctgc tttaagcatc aggcgcgaca gcagagcata ttctttgggc gtcagaatca
     6481 gttcttctcc gctcatccat acctggcggc gacccatatt cagcgtcagg ttgccaacgt
     6541 tcagctcact ttcgccctga ttattatggc gtcgtagcag ggcgcggata cgggcgtgca
     6601 actcttccag cgcaaaaggc ttcaccagat aatcgtcggc cccgacatcc agcccggcga
     6661 ttttgtcggt aagggtatcg cgggcggtga gaattaacac cggcagagtg tattttttct
     6721 gccggatacg ggcaagaaaa tgcagcccat cttcatcggg taagcctaaa tccagtacca
     6781 ccaggctgta atgaccggcc tcaaggcttt gttccgccat ccgcgcggtg ctcacgccat
     6841 cgcacgcgta gccttcggtt tgcgccgcca gaatcaatcc ctgcaataac agcgtatcgt
     6901 cttcaacaat cagaattttc attcactcac tctcctgcaa gtttgcagaa tatcatccgc
     6961 agcctggtaa tacttcgtct caacgccagt caggcccaat aaggttgaga ataaattgtc
     7021 ttgtgaatag tgttgcgttt gcgcctgttt ttgcaggcag ttctggtcaa cctgataccg
     7081 tttttgataa tcctccgata gccacagcag catcggcacc tgtttttggc tatccggggc
     7141 gatggcataa ggcagaccgt gcagatagat gccattttca cctaacgatt caccgtggtc
     7201 agaaagataa accaggctgg tggtaaattt atcctgatgt tctttcagca gattaatcgc
     7261 tttatcaaca atatagtcga cgtaaaccag cgtgttgtcg taagtgttca ccagttgctc
     7321 ttgggtacag gtctggatct cgttagtgtc gcaggttggg gtaaatttcc tgaactgcgg
     7381 cggatagcgg ttgtaatagg tcgggccgtg gctgccgatg gtgtgtaata caatgaggcc
     7441 atcgctttgc agattattga tgtactcttc cagcccgtgg aacagcactt cgtcatagca
     7501 ttcgccgttg atgcactgac caggcaggtt cagcgcggtg atgttctggt gaggtacgcg
     7561 atcgcaaacg cctttacagc cgccatcgtt gtcattccac agcacgttga tgcccgctcg
     7621 ctgaatgata tccagcacgc cttcctggtg ctgtgccaac tcttctttgt agtgctcacg
     7681 cggcatatcc gagaacatgc acggtactga aactgccgtt gccgtgccgc aagaggcggt
     7741 attagggaaa tagaccacgt tatcttttgc cagacgagga ttggtttcgc gtggataacc
     7801 attcagcgag aaattctctg cgcgcgacgt ttctccgacg atcaaaatgg tcaggttttt
     7861 acgtttccca ttttgcatta atgggttgcg gtgagcatct tcaccaatgc gtatcagtgg
     7921 caggttggct gaccgctggt gagagtacca cgaccagcta gcaacaatgc tgttggacgg
     7981 gcttaaggat ttcaccagct ctttgttgtt gcgaaacagc gaggcatagt ctttgtagaa
     8041 cagtgcggca accagcagaa tcagcagcac agaaatcagg atgttggccc cgcggaaaag
     8101 aacgctgcgc aggcgtgagg tggtgggttt gattttgatc cagcaggcaa taatcgcggc
     8161 caacacgcca cttaacccca gcgttaataa cattcgtggc gtcatcaacg cataactttc
     8221 cgccggcgta gtatcaatga tattggtgat cattgaacgg tcgatgacga tgccataggt
     8281 catgatgaaa tattgtgcag ccgcaccgac cagaataaac aggcaggcga gtggacgatt
     8341 aagccataag aaagagccta gtgtcaggac aatattaatc acgctgaatg cgacgaccgg
     8401 catcgacaag aaaaccagca cgttatgcag tgaatccagc ggtaacacct gtagcacctg
     8461 tttaaaaaag gcgatattca ggcagactga gatataaaaa gtagcaagta ataaccagga
     8521 gaataaattt aaagagggtc tttttagtag gcgcttcaac atcacggtgt ttccatcgaa
     8581 caaagtgcgc atatgctcgc aaagcaaaat taagccaacc ttaagttctt aaggttggct
     8641 tttatgtttg cttgattaag gggaattaat ctttgcttat tggtgcatct aagggatacg
     8701 ggtttttatg cagccggttg taattcagcg catacatggc ggtgattacc atcagagtga
     8761 caaatgacca cataacctct ttcgcgccgg accctactac ggcccagatg cagtagagga
     8821 aggcaatggt agtaactgcc aggtatgccg ggcgtgcttt accaaagtga ccgtggccga
     8881 gcagtagtaa cgccgcacag gtgtaaagat atggcaccag tgtaaagatg accgacacgg
     8941 aagaaaccag accgaattct ttggtcgcgt ttggcgaaat gctactgagc tggaagatgg
     9001 tcatcaaaat accgacgata atcagccccg ccactggcgt gcccgcttta tttacacggg
     9061 caaaaatcgg tgggaacagt ccgtcatcgg cagcggcttt cgccgtttga cccgccagca
     9121 acgtccagcc gcccagtgaa cctaagcaac ccgcagctgc gcagaaggaa acaatggccc
     9181 cggcggtgtc acccaacgcc atccgtgcgg catcaccgaa tggcgaagca gaaacgcgca
     9241 gtgcggcatt agggatcatc cccataatcg cggtggtaga aagtacatag caaacggcag
     9301 caatcaatac cccgccaatg gtggcgatag ggacgttgcg tttcgggttt ttcaccacac
     9361 ctgcggcaac ggaggcactt tccacaccga tgaacgacca cagcgtaacg ttaagggtac
     9421 tttgaatcgc accgaaggtg cccagaccgc tgacgttcca tgccgccata taggtttcac
     9481 cacggaacca gaaccagcca aatacggcaa tcccgacgat ggggatcagc gccagcacgg
     9541 tagcaacggc ctgtacgcgg gtgatcattt tcggaccgac aatgttcagc aggacgaaga
     9601 tccacagcac cacgacgcag gtgatggtta ataccaatgg gtctttcaga atcgggaaga
     9661 agtaacttaa atatcctacg ccaatgacca ccatggcgat attgccgatc cagcaggcca
     9721 gccagtagag gacgttggtt tgataaccga gaaacgggcc aaagcagcgg cgggcgtaag
     9781 cgtaagaacc accaggactt gggtcgagga acgacatttt ggcgtatacc atcgagagcc
     9841 ccagcgcacc gataatcgtc accaaccatc cataaatggc aatcccgcca gtggaggcca
     9901 ggtttgcagg taacagaaaa acacctgacc ccataatatt ccccgacacc atcagggtga
     9961 cggggattaa gcccactttg tgagcatcag catccgaaga cataattaaa ctcctgcgaa
    10021 ggcgagcttc gtgacaataa attacgtcat atcatacgcc tgcattcgat cgttgaatta
    10081 ttatcggccg ggttatcgct gatgccgtta ttaatatagc tcttcgttaa ggttaaaaat
    10141 caggcgacag tgcgttctct gtgctgacta acataatgca gcggcgtcat accgtagaag
    10201 tctttaaaga cagaaataaa gtacgacgta ctgttgtagc cgcaggactg tgatacctga
    10261 gagatatttt taccgtccat cattaattca tttacggcat agcgcatgcg gcaggtggtg
    10321 attatttggc tataactggt gttttcgctt ttcagctttt tctttaacag acttgggcta
    10381 agacataaac aactggctac catactaaga ttccagtctt tgtggatatc gctttcaata
    10441 atttgataaa cgctgtcact tacacaatta cgtaacatat acatcattag tgaaaggaat
    10501 tttttactgt cgagaaaacg ggatagcaca gtgaataata atgcacgtgt tcgttccttt
    10561 tctgactcag tttcagcggg catcatgcta tgttgcgcgg caacgcgaaa gacgtctggc
    10621 gtcaggcatg gcagggtgag gatgggcgta gcgctacgtt gccatactgg tatttgcgag
    10681 agatctttat tcagaaaccg gaggtaatct ttgatgatgt cgtgactaat atgagcaacc
    10741 aaagtgttat taagagagga gacgtcgata atattatttt cacaatttaa taacgccatg
    10801 tggttagctt taaggctaat agattctttc ccattcaccc ttatccagac atctttttca
    10861 gtcaataaaa caatacaagg ttggtcgctg caaatcctca tacatgtact cctgagtgcg
    10921 aataaaagac cagttctcgc taaagcgaag cgataccggg aaatggttat ataattgtat
    10981 gatgaatata aacatgcata aaaataacga gttttactaa tattacaaat ttttatagta
    11041 atttctcatg tacatatttt tttacaggct aaaaaaagta taaataacag gcaaaaaaaa
    11101 gcgcggggtg ggaccgcgca aaaagcatga cattttttgt ttgcagcaat ttttatgaaa
    11161 acgttgccat acttaaaagg cctcaagcca ggcggcgtaa tgttatttaa caattacgcc
    11221 ttcagcggaa tagtggttac gctttcacac acataacgtg gtaaataccg tcaataattt
    11281 ctgtcccttc tgtttcgtgt tcaaatccag ggaaatgatg gtcccaggat tgcagcgagc
    11341 gtaaataact tacttgcgga ctgtttttat cgccgaagtt ttcaccagac agcagcatcg
    11401 ggattcctgg cggatacggg ataactgagt ttgccgcgat acgtcctggc agattttcaa
    11461 tggataccag ttcgacattg ttgtcgacaa tcgcgttgta cgcttcacgc ggggtgattt
    11521 ccgccaccgg taggccggaa taggcttcat tcaaccgtgc gccagggttg ttttctttca
    11581 gccaggcaaa catggtgtca cccagatcgt gaatccccat gttcgcgtaa gtgtcaggat
    11641 attgttcaac aagttccggc atcacctgcg ccagcggtgt gttggcatca tagtggcgtt
    11701 tgaaggagca aagggtgtta accagagtcc cccatttccc acgggtcacg cccatagaga
    11761 acaggaacat aatctggaag tcagtggtac gggtaggcac aatgccgtgg cgaccaagcc
    11821 aggctgtgac cagcgccgcc ggaacaccgg tttcttccag ttcaccatct tcacccattc
    11881 ccggagcaag gatgctgact ttaatcgggt cgagcatact ccagttatcc ggaatatctt
    11941 tgaagccgtg ccagctttcg cccggatgca tgacccagca gtcctgaacg gtggtcagca
    12001 gtttggttgg tgcgtcagca aagtcatagg ttttgccggt ttgtgggtcg gtgacgactt
    12061 ctttgttcca cggtttgaag aaccagctac cgtcagcggt gaactcttta tacagccgcg
    12121 ccatcgcctg acggaaatca accgcttcgt cgatcacttc ctgggtcagt gacaggccgc
    12181 tgttgccgtc catcatcgac actgccacgt cgttggatgc gcagatggca tacagcgggg
    12241 aggtggtggc atgcatcatg taggcctggt tgaagcggga gaagttaatc gccccacgac
    12301 cttcacgtac atgaatataa gaggcctggg acagcgcatt cagcagtttg tgggtggagt
    12361 gggtggcgaa aacggtagga ccgttgtgat cgccaggttc gccgcgcatg gcatagtgat
    12421 cggcatagat cgggttgaaa cgtgcatagc cgtaccaggc ttcgtcaaag tgcagacgat
    12481 cggaggtttt ttccagcaga tcctgtgctt ctttagcgtt ataacacacg ccgtcatagg
    12541 tgcagttggt caccacgcag taagacggtt tttgcccggc tttgtctttg gtcagcgggc
    12601 tttcactgat tttcttctgc aaggtttcag gttgcatttc ctgcggatag attggcccga
    12661 taatgccgta gcggttgcgg cttggcacca tgtagaccgg tttcgcgcct gtcagcatca
    12721 agccttgttc gatggattta tggcagttac ggtcaacgac cactacatcg ttatcggtca
    12781 tgcaagcctg catgatggtg cggttagagc cggaagtacc gacgactacc gaccaggagc
    12841 gatcggcacc aaatacgcgt gcggcatatc tttcgctttc gccaaatgcg ccagtatggt
    12901 caagcaaaga accgagggaa gtgcgttcga tgcccatgtc ggtgcggaac agattttcac
    12961 cataatagtc atggtagaaa cgtccggcgg gtgttttggt aaaaccaacg ccgccctggt
    13021 ggcctggcgc tgcccaggaa tattcatgga tgtcgctata tttcatcagc gcgctgaaca
    13081 gtggcggcaa tagctgctgg cggtagcggg tcatcgcggc aacggcgcgt ccggcgataa
    13141 agtcggcggt atcttccaga atccaggcga attcatcgac aagctccagc agatcccggt
    13201 ccattgcggc gagggctttt tcccgatcgc ccaacaggaa gaccggcacg ttttgttggc
    13261 gctcatgaag cttaccaatc aattgtctga cgttttgatg ttcgtccgga tgttccattt
    13321 gatagctgaa catcaggcag tcaatggctt cgtttgaaga gagaatggca aaaccatcat
    13381 caaaggaggt ggatttaatc acggtaacat tttgctggct taaagcatct gccagacgct
    13441 caacggcgtt accgacccag gtgtcttgat ggagaaactc gctttcaaca attaatactt
    13501 tcatcattgc ttacccggtt atgaaggaat cttcgttgcg ggcaagtatc ttccggcctt
    13561 ttcgggctgt aaagcgcgtg aaaaacaaac atttttgaaa tatttatttt tataaacaat
    13621 acgttatgtg attattttaa attattttca ctgcttatta tcgacggcta aactattttt
    13681 ttggctgata ctgatatcgt ctttagccgg gaaacgtctg gcggcgctgt tggctaagtt
    13741 tgcggtattg ttgcggcgac atgccgacat atttgccgaa cgtgctgtaa aaacggctac
    13801 tcgaacgaaa gcctgccgtc agggcaatat cgagaatact tttatcggta tcgctcagta
    13861 acgcgcgaac gtggttgatg cgcatcgcgg taatgtactg tttcatcgtc aattgcatga
    13921 cccgctggaa tatccccatt gcatagttgg cgttaagttt gacgtgctca gccacatcgt
    13981 tgatggtcag cgcctgatca tagttttcgg caataaagcc cagcatctgg ctgacataaa
    14041 attgcgcatg gcgcgagacg ctgtttttgt gtgtgcgcga ggttttattg accagaatcg
    14101 gttcccagcc agagaggcta aatcgcttga gcatcaggcc aatttcatca atggcgagct
    14161 ggcgaatttg ctcgttcgga ctgttcaatt cctgctgcca gcggcgcact tcaaacgggc
    14221 taagttgctg tgtcgccagt gatttgatca ccataccgtg agtgacgtgg ttaatcaggt
    14281 ctttatccag cggccaggag agaaacagat gcatcggcag attaaaaatc gccatgctct
    14341 gacaggttcc ggtatctgtt agttggtgcg gtgtacaggc ccagaacagc gtgatatgtc
    14401 cctgattgat actcactttt tcattgttga tcaggtattc cacatcgcca tcgaaaggca
    14461 cattcacttc gacctgacca tgccagtggc tggtgggcat gatatgtggt gcgcgaaact
    14521 caatttccat ccgctggtat tctgaataca gcgacagtgg gctacgggtc tgcttttcgt
    14581 cgctgctgca cataaacgta tctgtattca tggatgactc tctttcctgg aatatcagaa
    14641 ttatggcagg agtgagggag gttgactgcg aataggcgca cggttttatt cctctccctg
    14701 gaggtgcgag gagagcggat gagaagatta gggtcgatta ttcagctttt caggtaattt
    14761 attcccataa actcagatct attgatgtgc cacttaggat ctgagttttt gggaatgctc
    14821 aaccgggaaa ccggaggttt tctgcgatat cgcctgccat gatgaagtta ttcaagcaag
    14881 ccaggagatc tgcatgatgt ctgcacccaa aattacattt atcggcgctg gttcgacgat
    14941 tttcgttaaa aatattcttg gtgatgtgtt ccatcgcgag gcgctgaaaa cggcgcatat
    15001 tgccctgatg gacattgatc ccacccgcct ggaagagtcg cacattgtgg tgcgtaagct
    15061 gatggattca gcaggggcca gcggcaaaat cacctgccac acccaacaga aagaagcctt
    15121 acaggatgct gattttgtgg tggtggcatt tcagattggc ggttatgaac cttgcacggt
    15181 gactgatttc gaggtctgta agcggcatgg tctggaacaa accattgccg atacgctggg
    15241 gccgggcggt attatgcgcg cgctacgtac cattccgcac ctgtggcaaa tttgcgagga
    15301 catgacggaa gtctgccccg atgccaccat gctcaactac gttaatccaa tggcgatgaa
    15361 tacctgggcg atgtatgccc gctatccgca tatcaagcag gtagggctgt gccattcggt
    15421 gcagggaacg gcggaagagc tggcgcgtga cctcaatatc gacccggcta cgctgcgtta
    15481 ccgttgcgca ggtatcaacc atatggcgtt ttacctggaa ctggagcgca aaaccgccga
    15541 cggcagttac gtgaatctct acccggaact gctggcggct tatgaagcag ggcaggcacc
    15601 gaagccaaat attcatggta atactcgctg ccagaatatt gtgcgttacg aaatgttcaa
    15661 aaagctgggc tacttcgtca cagaatcgtc agaacatttt gctgagtaca caccgtggtt
    15721 tattaagcca ggtcgtgagg atttgattga gcgttataaa gtaccgctgg atgagtaccc
    15781 gaaacgctgc gtcgagcagc tggcgaactg gcataaagag ctggaggagt ataaaaacgc
    15841 ctcccggatt gatattaaac cgtcacggga atatgccagc acaatcatga acgctatctg
    15901 gactggcgag ccgagtgtga tttatggcaa tgtccgtaac gatggcttga ttgataacct
    15961 gccacaagga tgttgcgtgg aagtggcctg tctggttgat gctaatggca ttcagccgac
    16021 caaagccggt acgctacctt cgcatctggc cgccctgatg caaaccaaca tcaacgtaca
    16081 gacgctgctg acagaagcta ttcttacgga aaatcgcgac cgtgtttacc acgccgcgat
    16141 gatggacccg catactgccg ccgtgctggg cattgacgaa atatatgctc ttgttgacga
    16201 cctgattgcc gcccacggcg actggctgcc aggctggttg caccgttaag gggtaatcgc
    16261 ttccggcgct ctggtgatta agccaaagcg ccgtagctgg cggcaatgaa agcgggcgag
    16321 gaatatcacc gtcgttaatg cgcccagtag tgcgcagaac atatcggatt gcgtatccca
    16381 cgggtcgcct tgagtaccga gaaaatcatc tgcgccctgg cccattgcca gcgctgccca
    16441 ccactcgatt aactcataca tcgcgcttat cgccagcgcg acacaacaga ccagaaacgc
    16501 caccatttta cgcccgcgaa cgtacatccc gcgcacgagg atctctcgtg ccaccagtgc
    16561 aggcaccagc ccctggaaaa aatgccccag tttgtcatac ggattacggc tcaaccccag
    16621 ccattcctgc acctcaaaac caacggggac tttcgcgtag gtgtattgcc cgccgaccat
    16681 caggatgatg gcgtgaaaga aaatgagcgt atagagcagc ggcgttaacg ggtaacgttt
    16741 agcggtggca agcagtagcg gcacgacaat aatcaccggt gtcacttcca tcagccaggt
    16801 gagtttgtca tgggccgaaa tgccggtata aatcaggatt aacgttagcg ccagtgcgcc
    16861 ggtgttaaga attaacggct tgagtgtgcg ggtcatggtg agttcgcaag tcagggaaaa
    16921 tactgactat tgaccagtaa gcgtgaaatt acaatcgcaa aagaggtggc ccagggggat
    16981 cacctggcag catgctgcca ggcgctgggc cgaagaggtt acttagtgca gttcgcgcac
    17041 tgtttgttga cgatctgctg gaagaagtcg ttacctttgt catcgaccag gataaacgcc
    17101 gggaaatctt ctacttcgat tttccagata gcttccatac ccagctccgg ataagcgacg
    17161 cattccagat gcttgatgct ctgctgcgcc agtaccgccg ccggaccgcc gatgctaccg
    17221 aggtagaagc cgccgtgttt atggcacgcg tcggtaacct gctggctgcg gttacctttc
    17281 gccagcatga tcatgctgcc gccgtgggat tgcagcagat ccacgtagga gtccatacga
    17341 cctgcggtgg ttgggccaag tgaacctgat ggataaccgg caggcgtttt cgccggaccc
    17401 gcgtagtaga tcgggtgatc tttgatgtac tgcggcagtt ctttaccggc gtcaatcagc
    17461 tctttcagct tggcgtgtgc aatatcgcga cccacgataa tggtgccggt gagcgacaag
    17521 cgggtggata ccgggtattg cgaaagctgg gcaaggatct ctttcatcgg gcggttaagg
    17581 tcgactttca ctgcttcgcc ttcacctgcc tgacgcagtt cttctggaat gtactggcct
    17641 ggattgtgtt ccagtttttc gatccagata ccttcgcggt tgattttcgc tttaatgtta
    17701 cggtcagcgg agcaggagac tcccatgcct accgggcagg atgcgccgtg acgtggcaga
    17761 cggataacgc ggatgtcgtg cgcgaagtat ttaccgccaa actgcgcgcc aagaccgagt
    17821 ttctgggcct cttccagcag ttcctgttcc agctggatat cgcggaacgc ctgaccatgt
    17881 tcgttccctt ccgtcggcag ttcatcgtaa tagtgagcgc ttgctaactt gacggttttc
    17941 aggttggttt ccgcagacgt accgccaatc acaaacgcga tatggtacgg cgggcaggct
    18001 gcagtaccga gggtacgcat tttctcgacg aggaagtttt tcagtttgcc gggcgtcagc
    18061 agggctttgg tttcctggta gagatacgtt ttgttggcag agccgccgcc tttagcgaca
    18121 cagaggaatt tgtactcatc gccatctacc gcgtacaggt cgatttgcgc aggcaggttc
    18181 gtgccggtgt tgacctcttt atacatgtcc agcggcgcgt tctgtgaata gcgcaggtta
    18241 tcttcgatat aggtgttata gacgcctttc gacagcgctt cttcatcacc gccaccggtc
    18301 cacacgcgct gacctttttt accgacgatg atcgcggtgc cggtatcctg gcaggtcggc
    18361 agcacgcctt tagcggcgat ttcggagttt cttaagaatt gcagcgccac gtacttgtcg
    18421 ttttcgctgg cttctggatc gtgaagaata gccgcaacct gtttctggtg tgccgggcgg
    18481 agcatgaaag aagcgtcgtg aaaggcttgc tgcgccagca gggtcagggc ttctggttcc
    18541 actttcagga tggtttcgcc gtcgaagtcg gcaacgctaa cgtaatcgga agtgagtaga
    18601 tagtattcgg tattgtcttt ccccatcggg aaaggtgcct ggtagataaa gggtttgttt
    18661 gacatagctt ccagcctgta actctgtatt tgttattcga aaatggcgtg ccgcgtagtt
    18721 acgggcggca cgcaaagtgc atttataaga acccgtacat cgcggcgaag atccagccga
    18781 agacgcacga tacgctcaca ccaatcaacc ccggcagaat aaagctgtgg ttgatgacga
    18841 agcgaccgat gtgggtggtg ccggaacggt caaactgaat cgctgccaga tcgctcggat
    18901 aagtcggcag gatgtaataa ccgtagcaag ccggtgctga agccacgatg tatgccggat
    18961 caacgccgat cgccagcgcg accggaacaa tcgccgccag cgctgcagcc tgagagttta
    19021 caaacttgga aaccagcagc agaacaatgg cataggccca cggatactct ttcaccattt
    19081 cacccagtac gccctgaatt tcagacatat gcgcaccgaa catggtttca gccatccatg
    19141 cgataccgta caccgccacg atggcgatca taccggaacg gaagacttcg ttttttgaga
    19201 tagacgcggg attggttttg gtcaggataa taatcagcgc cccggtcagc agcataaaca
    19261 tctgaataac cagtaccatc gacagcggtt tgccgccgaa ggatggacgc aggtccgaat
    19321 cagcaccaag aagggcgact acagcgattg ccccgaggaa gatccacatt gccagccagt
    19381 tgcttttcgg cagtttttta tccagcagcg tcgcggtatc accataaaca tactcacggt
    19441 tttccggtac ggagatgaat ttctggaact cttcgtcttt atccagatct ttaccgcgga
    19501 accagctgaa gataccgatc gccaggatac cgattaacgt cgatggaatg gtgattgcca
    19561 gcaggtcgag gaactcaaga tggcgaccat caaaggtgac attacccagc atcgcaacca
    19621 gagatacgac cgcaaccgac accggactgg cgataatccc catctgtgca ccgatagaac
    19681 ttgccgccat cggacgttcc ggacggatgt tgttcttaat agcaacgtcg tagatgatcg
    19741 gcagaatggt gtaaaccaca tgacccgtac cgcaaagaat ggtcagcgta caggtcacaa
    19801 acggcgcgac aattgagaca tatttcgggt tgcgacgcag cagcttctcg gcaatttgca
    19861 gcatgacatc aagaccgccc gaagcttgca aggtcgccga tgccgccacc accgcaatga
    19921 taaccagcat gacatcaacc ggtggtttac ctggctgaag gtggaagacg aagaccagaa
    19981 tgaccagacc gataccgcct aataaaccca gcgcgatacc cccctttctg gcaccataaa
    20041 acagacatat cagtattatg ataagttgga tagtaaataa catgtgtgaa ccctcgcgat
    20101 aatcctattt aaatttttgc tgaatagatc acagtcacgt tctgttttgt atgaactgtt
    20161 ttcagagcct gactaaatat ccgtttggtc gttaccggct tgagcaaata cctcacagtg
    20221 aatattggct gattaaagca gcggcaaatt tatgcactgt gataagcggc tttttcagag
    20281 agaaagactc tctggcgatg ggtttatagt ttgtacagcg cagatgttaa tgttttgttt
    20341 gctgttatct tattgatatt tatcggtctg ttttttgttc tcagggcagg tatcattagg
    20401 tttatagatt aatctgatct acccatttgt gggtaaaaat acacataatg tgggtgacat
    20461 aatagttaat taacttttgt tagcgttttg aaattaaaaa caccgttcat ctgaagagat
    20521 attaattttt agcgatgatg gagggataat tatatttgat ctggcacaag ttttactgat
    20581 gaacgatgta acttgtgcca gggggatttt gcattacggt aataattatt acttacagat
    20641 aaccagcgat ccgttattgg caatattgtt tcagtagtga gtagtgttct gcctgaatac
    20701 ggtaacggta aaccggacgc cccgtgacgc cataatggat actggtgaac aatatgtggc
    20761 agttgaccag ccagataagg tatttacggc aggaaacacg cgagatgtta acctcgttag
    20821 ccagctcgtc ggttgaaaac tcatagtcct gatgcgcgtc aatccactgg cacagcgtgc
    20881 gtaacgtctg cggcgttaag ccttttggca agcgacgagg atcctgctcg ttggagctgc
    20941 tgccgtggat tagctgatca agctcggcct ggtcataata ctgatgtttt tccagcgcca
    21001 ttttcttttg ccgccagccg gtgagcgcct cttcaaagcg ggaagcctgg aagggtttga
    21061 tcaggtaatc cacaacaccg taatgcagcg aatctttaat ggttactgca tcggcagcgg
    21121 aggagatgac aatcacatca cttttgcaac gcgcgttatg caggacaggc agtaaatcga
    21181 gcccgttctc tttttgcata tagatatcta gcaatatcag gtcgataggc gaatcgctat
    21241 tgaagataat ctctttggct ttctccagcg tcgaagccgt tccacagcat tgaaagcccg
    21301 ggatttgtgc cacgtatcgg cgattcagct ccgcgaccat tgcgtcatca tcgataatta
    21361 atacattgat catctgttcg acctctcccc gtcccagggt atctggacaa aaaattgtgt
    21421 gaaaatcccg ggttccgatt ccacggcgat gctgccgccg agattttcta cctgttgttt
    21481 gacaagtgct aaaccgacgc ctcgctcgct tccttttgtc gagacacctt tgtcaaaaat
    21541 gtgatcgatt ttgtcgggtg cgatccccgg tccatcatcg ttaacttcgc agtgcagcca
    21601 gccgtgacgg tagtgcaatg ttacgctaat ttcgccaccg ggttccggcc ctaatgcctc
    21661 cagcgcgttt tctatcagat ttcccaacgt ggtaatcagc gtcgcgacct gatcctcact
    21721 accgctgtct ggcagctggc tttcactgtt taaaatcagc gtatggccta aatcggtcgt
    21781 gcggttaatc ttgctgatta aaaaaccagc gataaccgga gatttgatct tacccagcag
    21841 agagccaatc tcttcctgat agttattggc tgttttgaga atgtaatctt ccaactgctt
    21901 ataactcttc agatgcaata atccgagaat cacatgcaat ttattcataa attcgtggga
    21961 tcgttcacga agtgcgtcag catagttgac cagaccgtcg agtcgctgca tcagtttacg
    22021 tacttcagtt ttgtccctga aggttgaaat ggcaccgatg ataacgccat tactgcgcac
    22081 cggaacggtg ttgatcagta atagtcggtc cttaatcgta atctcttcgt cgcggcgtgg
    22141 ggttccgtcg cgtaacactt ccgagacatc taccacctgt gaccacgagt ggcttagcgt
    22201 cgacagtttc tcatcgtcct gcgacttacg gtaattcagc aattcttgtg cggcatcgtt
    22261 gatcagcgtg acctcgccgc gatcgtccac ggcaacgacg ccttctttga tggactgcaa
    22321 catggcctgg cgttgctcaa acagtgtgga gatttcgtag ggttccaggc cgaaaaggat
    22381 ttttttcagt acgttaacca gaatgcatgt gccgatcagt ccgaccagca tgccaaatag
    22441 taccgaccag ataatgctcc agcgactgtc attgatctgt tgggtcacgc ggcttaactc
    22501 aaggccgatc gccaccacgc caatttgttt atgattttca tcgtagatgg gggtaaatac
    22561 gcgtaaagcc tgcgccagaa aaccgcgatt gatagcgaca ttttcttcgc cattcagcgc
    22621 tttaaggatg tcatcacctt taaatggctg accaatacgc tgggcttcag gatgcgagta
    22681 gcgaagactg tgcatatcgg taacgacaat aaacagcaga tcgttgcgtt tgcgcacggc
    22741 ttccgcgatg gcctggattc cactctcctg cggttttttc tgcaagccct gacggatttc
    22801 cggcgagtcg gcgagggtac gtgctactgc cagagccttg ttagctagcc cgtctcgcgt
    22861 catatcactg atttgcgaga agtaaatcag atgcaccacc aatagcaccg agaacagtac
    22921 cgcgctgacc attaagatca cagtggtact caatttcatc ggacgtttgc gtaacatacg
    22981 gtagggcaat gaatgtctca tcagcttcct tgtgtgacaa atttcttaag cattatctct
    23041 gatgaggcgg gtaattcaaa gggagtaaga atgattggct atatagggga agagattctg
    23101 gcaacggaaa ctgccagtgc tgtatgaaga ttccggggct atgcttatag cgataatcat
    23161 actgatgaga gagggaaggt catggatcgg gcgctactgg acgggggtta tcgctgttat
    23221 accggcgaaa agatcgatgt ctatttcaat actgcgatat gtcagcattc tggcaattgc
    23281 gtacgtggca acggcaagtt atttaatctc aaacgaaagc cgtggatcat gccggatgaa
    23341 gtcgacgttg ccactgtggt taaagtgatt gatacgtgcc cgagcggcgc gctgaaatac
    23401 cgtcataaat aagcgagggt aaaatggaaa tacgcgaagg ccacaataaa ttttacatta
    23461 atgacgaaca aggcaagcaa atcgctgaaa ttgtctttgt gccgaccgga gagaatttag
    23521 cgattatcga acataccgat gtcgatgaaa gcctgaaagg gcaagggatt ggtaaacagc
    23581 tggttgcgaa agtcgtggaa aaaatgcgtc gggaaaaacg aaaaattatc ccactatgcc
    23641 catttgcgaa acacgaattt gataaaacac gggagtatga tgatattcgc agttgatggg
    23701 agagtacaga gtcacgatat ttttcatgct ctccgcggtg tgatgcagga gagcatctga
    23761 agggtagggg gatgcacaaa gaatggtcag agagagcgtt tttttgtccc aagtcatccc
    23821 ctttactgag caaaaaaaaa agaatatctc ctatatgaga atcatcgatc ggggttaata
    23881 agttttgcgt ccccagagtg ttcaatattg ataggagtca tattatggaa ggtaaaaaca
    23941 agttcaatac ttatgttgtt tcttttgatt atccatcatc ttattcctca gtgttcttaa
    24001 ggttaagatc attgatgtat gatatgaatt tctcctctat cgtggctgat gaatatggga
    24061 taccacgaca attgaatgaa aattccttcg caataacgac atcgttagcc gcaagtgaaa
    24121 tcgaagattt aatcaggctc aaatgcttag acttaccgga tattgatttt gacctcaaca
    24181 ttatgacagt tgatgactat ttccgtcagt tttacaagta gccaggaagg ataagaagaa
    24241 cataaaaaat ggcactattc tctaaaatat taatttttta tgtgattggt gtgaacatat
    24301 cctttgtcat tatctggttt atctcacatg agaaaacaca tattcgttta cttagtgcat
    24361 ttctggtcgg aataacctgg ccaatgagtc ttcctgtggc attacttttt tctctctttt
    24421 aggagctgta gttgagtgga cccgctggtt atcttgaaac gaagcgtctc tcctcaaaac
    24481 tggatttgcg gaatgacgaa cgaagcagtc gcctgactgc ttcgttcatt aaagtgaaat
    24541 ttatttctgt gggcgcatcg ccgggaagag aataacgtcg cgaatagtat gactgtttgt
    24601 aaacagcata atcattcggt cgataccaat acccagacca gcggttggcg gcagaccata
    24661 ttccagcgca gtcacgtaat cttcgtcata gaacatggct tcgtcgtcac ctgcagcttt
    24721 agcgttaacc tgttcctgga aacgttcagc ctgatcttct gcgtcgttta attcgctaaa
    24781 accattacca atttcacgac caccgatgaa gaattcaaaa cggtcggtga tttccgggtt
    24841 aacatcatta cggcgcgcca gcggggacac ttctgccgga tattccgtaa taaaggttgg
    24901 ctgaatcaga tgtgcttctg ccacttcatc aaagatctcc gtaacaatac gtcccagccc
    24961 ccagcttttc tctaccgtaa taccgataga ttcagctaat gctttagcag catcaaaatt
    25021 atccagatcc gtcatatcgg tttctggacg atattttttg attgcttcgc gcatggtgag
    25081 tttttcaaac ggtttgccga aatcaaacac atgctcgcca taagtgactt tagtggtacc
    25141 cagtacctct tgtgccaggg tgcggaacag tgactccgtc agttcaatca aatcgtggta
    25201 atccgcatat gccatgtaga gttccatcat tgtgaactca ggattatggc gaacagaaat
    25261 accttcatta cggaagttac ggttgatttc gaatacccgt tcaaaaccgc ctacaaccag
    25321 acgtttcaga tacagctccg gcgcgatacg caggtacata tctaaatcca gagcgttatg
    25381 atgggtaata aacgggcgag cagatgcccc acctggaatt acctgcatca tcggggtttc
    25441 tacttccata aagccgcgcg cgaccatgaa ctgacggata gcggccagaa tttttgaacg
    25501 gacaacaaac gtttgacggg atttatcgtt agcgatgagg tccagataac gttgacgata
    25561 acggacttcc tgatcctgca gaccatggaa tttatctggt aaaggacgta gtgctttagt
    25621 cagcaggcgc agctcagtac agtgaatgga aagctcaccc gtttgcgtct taaacagcgt
    25681 accgcgggca ccgataatgt cacccagatc ccatttttta aactgatcgt tataaacacc
    25741 ttctggcagg ctatctcttg caacgtacag ttgaatacgg ccaccgacat cctgcaacgt
    25801 tacaaaggag gctttcccca taatacgacg ggtcatcatt cggccagcaa ccgagacttc
    25861 aatgtttaag gattccagtt cctggttatc cttcgcatca aactcttcgt gcaactggtc
    25921 agaggtatgg tcgcggcgaa aatcattggg aaacgccaca ccttgctgac gtagtgccgc
    25981 cagtttttcg cggcgatttc tcagttcatc gttaaaatca atagcctcat tggctccccg
    26041 tgtttctcgt tcagacattt tggttcctct aaatccagct ttcaatttag cgtagataaa
    26101 gagacagatc ggtacactgt attatctgcc tcgactatta ataactcagt ttatcttatc
    26161 gaaactaacg cttacctcaa gttgatatca ctaataaata accatcaaaa tggtttaaat
    26221 gacttattaa tctcgattta ctagtgtgat aaactgaaag ttaatttatt cataaccaat
    26281 tgtttttacg acagttaatc gttgctctcc tgtatcatat tcgtcggcgt gctaaacaga
    26341 aaacgggtag caaaggccaa taccacgatg atcgcgacac aagccaacgt ccactctccc
    26401 atctgagaaa agaatcgctg gtaagcggca attgccatac cgctaatttg cgactctgtc
    26461 gtctgctgtg ccacgacgcc tgccagccag ttggcgactg cgcctgttgc cagcatataa
    26521 ataccggtta atacgccaga cattttcaga cgcgtgattt gcgcaatcgc caccgggtca
    26581 ataaagagtt cagcaaagcc cattagcgcc agcccggata tcatcacgcc cattgacgct
    26641 tgaccgccag ccgctgcatg tcgggcatca aatgccagca acataaagcc acaagccatc
    26701 agcagtaagc caaaggcaaa cttcagccag acgcgcaatg ctgaattgcc gcggctttct
    26761 ggcgacgcca gccaggccag aacaacccca gcgagcatca ccgcaatggc attcaccgac
    26821 tggaatagtg ctgtaggtac ttcaatattg aatgcctgac gattcacaaa gcgatcgata
    26881 aacaagctga tggtactgcc gccctgttgt gccagtaccc agaacaacgt cccgacaaac
    26941 atcaacaata caatttgcca aagagcacgg cggtgttcgg ggaatttgat catcatgcgg
    27001 gcaatgattt gtgcggcaat gaggcaaacg atcgccagca aatatccgga ccagtcgttc
    27061 tccagcagca gggtaaaaaa tacaggggct aaacagagca tcaccactaa ccagctccat
    27121 actggtaagg caaatttgac gcttgtgagc gcttttttat ccatactacg cgtggactgg
    27181 aaatgacgat gaccacttaa gaaaatcaac aaaccgataa acatgccgcc tcccgcaagg
    27241 gcaaagccaa catgccatcc ataccactga gcagccaggc cgcaggcgat gggcgcggcg
    27301 atagaaccga tattgcccgc agcatacagc agcgaaaaac cgccatcacg tcgatgatcg
    27361 ttctcgtcgt agagctcgcc aagcaaacag ctgatgtttg atttgaataa accgtagcca
    27421 caaataatga ttgccagcgc cagatacagg ctaaaggttg aatttgtatc aatgcccagc
    27481 accacatggc caagggtcat taacagcgcg ccggcaatca ctgcagtgcg gttgccgagc
    27541 aggcggtcgg caagccagcc gccgagaata ggggtaacgt aaaccagaga agcatatgcg
    27601 ctgaacaggc tgatggcatg gttatcatca aaaccaagct gatgggtgag atagagaatg
    27661 agtaaggcac gcatgccgta aaaactgaag tactcccaga tttggatcgc cacgatatag
    27721 tatatcgcgc gcggctgtga gggtgttttc atgtgttctc cttatgagca aaaaagggaa
    27781 gtggcaagcc acttcccttg tacgagctaa ttattttttg ctttcttctt tcaatacttt
    27841 aacggtatag cggccatcag cctgacggta tgcaccgtga atatcggttt caaagcccgg
    27901 atagtgagcg ccgatttcac acagcatctg caggaactcc agaaccggac ggctttcttc
    27961 ggtgatcatt tcacccggca ttaccagagg aactcccggc ggatacggaa ggatcatatt
    28021 ggcgttaata cgaccgacca tttcgtcgag gtaaacttct tcggtcatac cgtgcagctc
    28081 tttctggaac gcagcatacg gagtcattac catagtcggc agaacttcaa atgcgcgata
    28141 catcagatcc ggcagattgt ggtgaacaat cagtttgtgg atattctgag ccagttcctg
    28201 aatacgcatg ttttcataga attcaggatc ttcacgatac agagacggca gcatgttttt
    28261 cacacgcagg ttcagatcga acgcacgttt gaagtcagtc agagcacgca gcaggctcag
    28321 tgctttggtc ttatcgatac cgatgctgaa caggaacagc aggttatacg gaccggtttt
    28381 ctcaacaacg atgccatgtt cgtcgaggta tttcgccacg atgctggccg gaataccaaa
    28441 gtcgctcatg gtgccgtctt tttccatccc cggagtcagc agggtgactt tgatcgggtc
    28501 aagatacatg tgctcgttat cgatgttttt gaagccgtgc caggtgctgt cagaacgcag
    28561 cggccagcat tcagtcgtat cgatatgatc cggctgccat acatcaaaga accagccatc
    28621 agattccgtt ctcagacgtt tgatctcttt acggaatttg atcgcacgtt caatggaacc
    28681 attgatcaga cgtttacctg cattgccttt catcatcgcc gcagcggttt cagtggacgc
    28741 cacgataccg tagtgcggag aggtggtggt gtgcatcatg taggcttcgt taaaggtttc
    28801 ttcgtttacg tcacctttaa cgtggatcat ggaagcctga gagaacgccg ccagcagttt
    28861 gtgagtagac tgggtttcgt aaatcacttt cccttctaca cggccaccgc tcataccgca
    28921 tttaccttcg taaatcggtg agaagttggt gtaaggcacc cacgcggagt caaagtggat
    28981 ggatttcaca tccagtgttt tcttgatgaa gtcggtgttg tacagcagac catcataggt
    29041 agagttggta attacagcat gtaccggcca ggttgcgttt ggtgtttctt tcacgcgctt
    29101 agcaatggta gcgtgctgga attcactctg tgggatacca ccaagaatac cgtaagcgtt
    29161 acgggtcggg cggaaataga ttggcgtaac atcgctcatc atcatcaggt gggtcagcga
    29221 tttgtggcag ttacggtcaa tcagaatggt gctgcctgcc ggagcagagt acataccaac
    29281 aattttgttc gcagtggaag taccgttggt caccatgtag ctgcggtctg cgttaaagac
    29341 gcgagcgata tactgttctg cttctttgtg tggaccactg tgatccagca gagaacccaa
    29401 ttcagatact gaaatggaaa tatcagattt catggtgttc ggaccaaaga aatcatagaa
    29461 caggctacct accgggcttt tctggaatgc agtaccaccc atgtgaccag gagtacagaa
    29521 agtatattta ccttcacgaa catatttaaa cagtgctttg gtcagcggag gcagaatagt
    29581 gttgatatat tcgtcagtgg tctgcttgat cttgttagca atatcatcag cagcacccag
    29641 cgcatattca aagaagctaa tctgtaaacg caggtcattc aggcttacat cgagagtgga
    29701 atacgtatta gcgaacgcgt acaacggcag gttctcgttc attttgctaa tttcttcgca
    29761 cagctcgaga ttatatttat cccagtcaaa aataacgccg cacagacgcg cattgttttc
    29821 gatcagtttt aataagtcgt cacggtcgtt cgggtaaaca atctggaagt tcagacgttc
    29881 aagcgcgcga tgaagttcac ggatgggttc ttctttaaaa taaaccccca tgtgattcaa
    29941 tattgcaata acgttcatag tcatatctcc aggtaaaaaa ggcccctccc aacacatggg
    30001 acaaaatgaa aggaggagcc tcggaaaata cttttaatta atgtgcgtta gacgctgtgt
    30061 ggttatccat tgagtggctc tggcgctcgt gcattttgcg agcgtagaac atcaggataa
    30121 tcaggctgac gatgaaggta cctgccagct cgaaggagct tgcgcccatc agcgcgatga
    30181 agcagaacac gcaacccagt acagagcaga tcaggctgac aaagttgcgg atgttaacgc
    30241 cttcaaaacg aatcaggtca acgcaagagt agaagtacgg cagcatagtc agcagtactg
    30301 cgataccggt cagttcaccg aacaggtcag atgctttacc accggcagag ttcatcagag
    30361 tgatgaggat catcagggca gtcattttca ctgcagccag cagcagacct tttttcggaa
    30421 taccgttgct gtcgacttca ccataaactt tcgggaagtt accgtcgtta gcggcacgta
    30481 cacctgcctg gcctaccaac atcatccagg agcccagaga agtcagacac gcaaaggcgg
    30541 tgaatgcaga aaccagtggc gcagcccagt taccgaggat agttgaagca ctgattgcaa
    30601 acggagcacc ggaagccgcc attacagaag acggatacat accggaaagc acctgagtcg
    30661 cagcgatgta aacaatacct gctaaaccag tacccagcat ggttgccagc ggaacggtac
    30721 gtttcgggtt tttaaccata ccagtactta ctgctgcgga ttcaacaccc acgaaggccc
    30781 acaggcagag cagaatactt ttaatgatcg catgaccatc agtggtatcc gcagtattcc
    30841 agttagctgc ataagttgct gcatcaaacc aatgccagcc aacaatagca gtcatcacca
    30901 caggaataag aaccagcacc agaccaatag tggttaaacg gcttacccag gtaccgccga
    30961 gcatatttac aaaggtaaat acccagacga tagcaataca ggcgataccc gccggaacag
    31021 gatcatttaa tactgggaag aaggtggaaa gataagatac agcggtaata ccaattgcca
    31081 ggttaccaat ccagttagca tggtaataaa gaacacctgt ctgaaaacca aatgcagggg
    31141 aaatttctcc ggcatacgca attgggccac cttgttgcgg gttttttgtt gccagtcggg
    31201 catatacata tgccagcgac attgcaccaa taatagagat aatccaaccc cagatagcaa
    31261 taccaccgat acttgctagg ttcgcaggta ataatgcaat accgctcccc atcatattac
    31321 cggcaacaac accggtacag gcaaatagcc cgatcttctt ggcagaactc atgctcttct
    31381 cctaatttca tttttgaatt tggagtccgg gtcatgatgt ataactattt cctgaccaga
    31441 ccaaactggc gctaagatta ctcacgaaaa aaggattaat cctaaagatt aggtgaaata
    31501 acacaaaagt ttctgtaagt gagaacttga ggttttttat taacacatca ggatcgcaag
    31561 ttgatatcat gaaaa
//
LOCUS       DAZKHK010000053        31400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKHK010000053 DAZKHK010000000
VERSION     DAZKHK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.053"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..533
                     /gene="uspC"
                     /locus_tag="ACHOS5_002388"
     CDS             105..533
                     /gene="uspC"
                     /locus_tag="ACHOS5_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP9777201.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
     gene            complement(540..1964)
                     /gene="otsA"
                     /locus_tag="ACHOS5_002389"
     CDS             complement(540..1964)
                     /gene="otsA"
                     /locus_tag="ACHOS5_002389"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP9777202.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVTKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1939..2739)
                     /gene="otsB"
                     /locus_tag="ACHOS5_002390"
     CDS             complement(1939..2739)
                     /gene="otsB"
                     /locus_tag="ACHOS5_002390"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP9777203.1"
                     /translation="MTEPLTETPELSVKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2906..3892)
                     /gene="araH"
                     /locus_tag="ACHOS5_002391"
     CDS             complement(2906..3892)
                     /gene="araH"
                     /locus_tag="ACHOS5_002391"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP9777204.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3907..5421)
                     /gene="araG"
                     /locus_tag="ACHOS5_002392"
     CDS             complement(3907..5421)
                     /gene="araG"
                     /locus_tag="ACHOS5_002392"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP9777205.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5491..6480)
                     /gene="araF"
                     /locus_tag="ACHOS5_002393"
     CDS             complement(5491..6480)
                     /gene="araF"
                     /locus_tag="ACHOS5_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP9777206.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKASDIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7277..7780
                     /gene="ftnB"
                     /locus_tag="ACHOS5_002394"
     CDS             7277..7780
                     /gene="ftnB"
                     /locus_tag="ACHOS5_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP9777207.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7859..8110)
                     /gene="yecJ"
                     /locus_tag="ACHOS5_002395"
     CDS             complement(7859..8110)
                     /gene="yecJ"
                     /locus_tag="ACHOS5_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP9777208.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8225..8311)
                     /gene="azuC"
                     /locus_tag="ACHOS5_002396"
     CDS             complement(8225..8311)
                     /gene="azuC"
                     /locus_tag="ACHOS5_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP9777209.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8574..8897
                     /gene="yecR"
                     /locus_tag="ACHOS5_002397"
     CDS             8574..8897
                     /gene="yecR"
                     /locus_tag="ACHOS5_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP9777210.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9069..9566
                     /gene="ftnA"
                     /locus_tag="ACHOS5_002398"
     CDS             9069..9566
                     /gene="ftnA"
                     /locus_tag="ACHOS5_002398"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP9777211.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(9604..9843)
                     /gene="yecH"
                     /locus_tag="ACHOS5_002399"
     CDS             complement(9604..9843)
                     /gene="yecH"
                     /locus_tag="ACHOS5_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP9777212.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            10034..11245
                     /gene="tyrP"
                     /locus_tag="ACHOS5_002400"
     CDS             10034..11245
                     /gene="tyrP"
                     /locus_tag="ACHOS5_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP9777213.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11296..11961)
                     /gene="yecA"
                     /locus_tag="ACHOS5_002401"
     CDS             complement(11296..11961)
                     /gene="yecA"
                     /locus_tag="ACHOS5_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP9777214.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12157..12243)
                     /locus_tag="ACHOS5_002402"
     tRNA            complement(12157..12243)
                     /locus_tag="ACHOS5_002402"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12207..12209),aa:Leu,seq:taa)
     gene            complement(12256..12329)
                     /locus_tag="ACHOS5_002403"
     tRNA            complement(12256..12329)
                     /locus_tag="ACHOS5_002403"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12295..12297),aa:Cys,seq:gca)
     gene            complement(12383..12458)
                     /locus_tag="ACHOS5_002404"
     tRNA            complement(12383..12458)
                     /locus_tag="ACHOS5_002404"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12423..12425),aa:Gly,seq:gcc)
     gene            complement(12610..13158)
                     /gene="pgsA"
                     /locus_tag="ACHOS5_002405"
     CDS             complement(12610..13158)
                     /gene="pgsA"
                     /locus_tag="ACHOS5_002405"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9777215.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13215..15047)
                     /gene="uvrC"
                     /locus_tag="ACHOS5_002406"
     CDS             complement(13215..15047)
                     /gene="uvrC"
                     /locus_tag="ACHOS5_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9777216.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15044..15700)
                     /gene="uvrY"
                     /locus_tag="ACHOS5_002407"
     CDS             complement(15044..15700)
                     /gene="uvrY"
                     /locus_tag="ACHOS5_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP9777217.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            15996..16172
                     /gene="yecU"
                     /locus_tag="ACHOS5_002408"
     CDS             15996..16172
                     /gene="yecU"
                     /locus_tag="ACHOS5_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP9777218.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16159..16383
                     /gene="yecF"
                     /locus_tag="ACHOS5_002409"
     CDS             16159..16383
                     /gene="yecF"
                     /locus_tag="ACHOS5_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP9777219.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16451..17173)
                     /gene="sdiA"
                     /locus_tag="ACHOS5_002410"
     CDS             complement(16451..17173)
                     /gene="sdiA"
                     /locus_tag="ACHOS5_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP9777220.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(17403..18155)
                     /gene="tcyN"
                     /locus_tag="ACHOS5_002411"
     CDS             complement(17403..18155)
                     /gene="tcyN"
                     /locus_tag="ACHOS5_002411"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP9777221.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18152..18820)
                     /gene="tcyL"
                     /locus_tag="ACHOS5_002412"
     CDS             complement(18152..18820)
                     /gene="tcyL"
                     /locus_tag="ACHOS5_002412"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP9777222.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(18835..19821)
                     /gene="dcyD"
                     /locus_tag="ACHOS5_002413"
     CDS             complement(18835..19821)
                     /gene="dcyD"
                     /locus_tag="ACHOS5_002413"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP9777223.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(19926..20726)
                     /gene="tcyJ"
                     /locus_tag="ACHOS5_002414"
     CDS             complement(19926..20726)
                     /gene="tcyJ"
                     /locus_tag="ACHOS5_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9777224.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(20814..21365)
                     /gene="fliZ"
                     /locus_tag="ACHOS5_002415"
     CDS             complement(20814..21365)
                     /gene="fliZ"
                     /locus_tag="ACHOS5_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP9777225.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(21411..22130)
                     /gene="fliA"
                     /locus_tag="ACHOS5_002416"
     CDS             complement(21411..22130)
                     /gene="fliA"
                     /locus_tag="ACHOS5_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP9777226.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(22450..24237)
                     /locus_tag="ACHOS5_002417"
     CDS             complement(22450..24237)
                     /locus_tag="ACHOS5_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP9777227.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGSGTIANKAATISDLTAAKMDAATNTITTTNNALTASKALDQ
                     LKDGDTVTIKADAAQTATVYTYNASAGNFSFSNVSNNTSAKAGDVAASLLPPAGQTAS
                     GVYKAASGEVNFDVDANGKITIGGQEAYLTSDGNLTTNDAGGATAATLDGLFKKAGDG
                     QSIGFNKTASVTMGGTTYNFKTGADAGAATANAGVSFTDTASKETVLNKVATAKQGTA
                     VAANGDTSATITYKSGVQTYQAVFAAGDGTASAKYADNTDVSNATATYTDADGEMTTI
                     GSYTTKYSIDANNGKVTVDSGTGTGKYAPKVGAEVYVSANGTLTTDATSEGTVTKDPL
                     KALDEAISSIDKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMS
                     KAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            24503..25909
                     /gene="fliD"
                     /locus_tag="ACHOS5_002418"
     CDS             24503..25909
                     /gene="fliD"
                     /locus_tag="ACHOS5_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9777228.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFFATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTVIATSDSKLTIQQGGDKDPIAIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQNSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            25934..26344
                     /gene="fliS"
                     /locus_tag="ACHOS5_002419"
     CDS             25934..26344
                     /gene="fliS"
                     /locus_tag="ACHOS5_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9777229.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQPGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            26344..26709
                     /gene="fliT"
                     /locus_tag="ACHOS5_002420"
     CDS             26344..26709
                     /gene="fliT"
                     /locus_tag="ACHOS5_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP9777230.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLLLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            26787..28274
                     /gene="amyA"
                     /locus_tag="ACHOS5_002421"
     CDS             26787..28274
                     /gene="amyA"
                     /locus_tag="ACHOS5_002421"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9777231.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPTRAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(28308..28721)
                     /gene="yedD"
                     /locus_tag="ACHOS5_002422"
     CDS             complement(28308..28721)
                     /gene="yedD"
                     /locus_tag="ACHOS5_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP9777232.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            28908..30113
                     /gene="yedE"
                     /locus_tag="ACHOS5_002423"
     CDS             28908..30113
                     /gene="yedE"
                     /locus_tag="ACHOS5_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP9777233.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            30110..30343
                     /gene="yedF"
                     /locus_tag="ACHOS5_002424"
     CDS             30110..30343
                     /gene="yedF"
                     /locus_tag="ACHOS5_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP9777234.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            30452..31122
                     /gene="yedK"
                     /locus_tag="ACHOS5_002425"
                     /pseudo
     CDS             30452..31122
                     /gene="yedK"
                     /locus_tag="ACHOS5_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         7891 a         8022 c         7407 g         8080 t
ORIGIN      
        1 accagactaa tatttacctg tttaactgag ttgggattgc gtcgtttctc cattaggagt
       61 aaagctttaa tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata
      121 ttcttgtcgc cgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta
      181 tcgccaggcc agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt
      241 acaatcaatt agctgcgccg atgcttgaag atttacgcaa tgtgatgcag gaagaaacgc
      301 aaagctttct tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg
      361 cctacggtga gttaagcgaa catattctgg aagtttgtcg caaacatcat ttcgatttgg
      421 tgatttgcgg taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag
      481 tgattacctc aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgcacc
      541 tacgcaagct taggaaaggt agcaacttta tcgcgctgct ggctttctgc gcttcgcgga
      601 actatctgct ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg
      661 ataacgtcca gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca
      721 cgatccagcg cagctgcaac ttcgtcacga tcgtagggat taacaattaa cgccgacgtt
      781 aactcgtttg ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc
      841 tgagcggcaa catactcttt tgtcaccagg ttcatcccgt cacgcagtgg cgtcactaag
      901 cccacgtcag agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa
      961 taataaagcg gcgtccagcc taattgccca tatttaccat taattcgtcc ggcttcattt
     1021 tcgagctgat gacgaatatc ctgataggct tgcacatcac cacgagacgt tggcgctatc
     1081 tgggtataac gaattttacc gtgatgttgc ggatattttt ccagtaacgc ttcataggcg
     1141 agaaaacgct ccggcaaacc tttggaataa tccagtcgtt cgacagaaaa gatattttgt
     1201 acgtttttca gttctgcttt gagttgcgcc agttttggcg gcagtggacc ggcagcctgt
     1261 ttcgctattt ctttcggctc aatgccgatc gggtaaactt ccgttcgaaa cgctttgccg
     1321 caggccgtat ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aaggcaatcc
     1381 aggaacgcca gacgatcgtt ctccgtctgg aaacccagca aatcataatc acaaagctgt
     1441 tcaagcaagg tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata
     1501 tgcagaaaga aaccaatgcg attattcacg ccccgtttgc gtaattcatg cgcaaagggc
     1561 aacaggtgat aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta
     1621 tctgccagca acgcatttac gcgtagatag ccatcccagg caggacgctg aaattgcacc
     1681 agatcgagcc gataatgaaa agcgggccag agaacggcat tggagaattt gttgtagtat
     1741 tcgtcaaggt cctgttcgct gaggttaaaa gaggcccacg taatgttacc ctttttcacc
     1801 ttttttagcg gctgatcctc attccctgtt tcaccgctcc agccaaacca cagtccacct
     1861 gcggctttca gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg
     1921 tctggtggtg caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt
     1981 attttctctt ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg
     2041 cacgcccgcc agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc
     2101 gcccagccgg ttaacgactg cgaagccaga ttcatcggtc agatcatcgc ccagaaatac
     2161 gggcgttcgc ccgataaagg gagcttcctg cataaaggca gcaatcgcct cacctttact
     2221 ggtacctttc ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca
     2281 gatctgagta atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctggcg
     2341 ataatgcagc gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgggcgat
     2401 gaccgtatgc agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg
     2461 cgttttacca ttaatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata
     2521 aggttttgcc agtgcgtcaa gctccaccat tgagcgccct gaaatcaatg ccaatgcacc
     2581 atcacttgcg gttgccagta gctgaagtcc ttgcagaata gtgtcaggca cgacgacctg
     2641 atcgggatgc ggtttgattt ccgccagcgt tccatcgaga tcaaaaaacc aggcatattt
     2701 cacggatagt tcaggggttt cggttaacgg ttctgtcacc cggttctcct ccttctcttt
     2761 tcttattgtg ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg
     2821 gtcgccattt gcaacgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa
     2881 cgttaaattg ttgtggaaaa aagcatcaga cagtgcgttt tgctttttgc ttgtaacggt
     2941 cgaaaatcac cgctgccagc aggattaagc cgcgaactac gtactgtgcg aaaggagaaa
     3001 tattaagcag gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat
     3061 atgagatttt tccgatgccg cctttcagag aaacgccacc taaaacgcag gcggagatca
     3121 caatcagctc atagccaatc gacgtcattg gctgcccact ggtcatacgt gaagccagaa
     3181 taattccggc tatcgctgat accaggccag agagaacaaa gataataatt ttggtgcgaa
     3241 caaccggtac acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtattac
     3301 gaccaaaggt ggttttattc agcagcaagc caaagatgat caaacacgcg acggtgagcc
     3361 agattggcgc aggcagacca aaccagttgg cgtaaccaag ggcaaagaag ctttcatctt
     3421 cgataccgac cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg
     3481 ttgccagtgt cgtgatcagg gcatttattt tgagtttggc gataacaaag ccattgacca
     3541 gaccacagag aatgcccagc agcaaccccg ctgccacgcc aatccacagg ctttcagtca
     3601 gattaataac caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt
     3661 caaagtcacc ggaagcaagg cagaacaaca tgccacaagc caccatcccc gacatggaaa
     3721 ttgccaggcc caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac
     3781 aggcgataaa aagcaccgca aacaccacca gcatgccgta ctgatcccag atacgcccga
     3841 agctgaatga cgacttaggt gcgccagaac ccgatgtaga aacagaagac atcatactct
     3901 ccttactcag gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc
     3961 atctgcctgc tcgtgtaaca attcaccggc gatttcgcct tcccgcatca ccacaatccg
     4021 gtcggcaacg ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacaccctg
     4081 cgccgccagc gcataaatca cgttgtaaat ttcatgctta gcaccaacat caatgccacg
     4141 cgtaggttca tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc
     4201 tttttgctga tttccgcctg agagattcat gatcagttgc tcagcgcccg gcgttttgat
     4261 gttgagcgaa cgaatgtggt gatcggcatt gttttcttcc caaccgttgt tgattacaca
     4321 accgccaagc acatgtttac gtctggcact gatgttgata ttgtcgcgaa cagagtgcac
     4381 gggaataatg ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc
     4441 gtggctcggt ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat
     4501 ttgcgtcccg ccaaacatgc ctttcattaa ttcgctacgc cctgccccga ccagaccaaa
     4561 cagaccgaca atttctccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc
     4621 tttcacagca tcaagacgca gccgctcctc gccataacta cgcggttgcc agccgtagat
     4681 atcgccaatg tctcgcccga ccatcgcctg caccagtgcg tcgtggtcaa cctgctgcat
     4741 atcggtaaag gttttgacat aacgcccatc tttaaagacg gtgatggcat cgctgagggc
     4801 aaatatttct tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc
     4861 acgaataacg cggaaaagat tgtcgatttc gcgggcagag agggagctgg ttggctcatc
     4921 aaaggcgata attttggcgt tacgcgccag cgctttggcg atttcaacca tctgccactg
     4981 accaatggag agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaat
     5041 ttgtaaacct gcctcataat tcagcaatga acgattcaca atgccgcctt tatgcggcag
     5101 ctggccgaga tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta
     5161 aataatcgcc acccccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc
     5221 attaatcact acagaacccg tggttggcgc atagtttccg ctgaggattt ttaagagagt
     5281 tgattttcct gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa
     5341 actaatatcc gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag
     5401 atacggggta gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg
     5461 catcatgcag aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag
     5521 ttcttcttta aagttgtcac gcgtgatcag caccacgtcg gtaacttcgg tgaatttggt
     5581 cggttcaaca ccttttgcaa cccagttgta aagcatttcg ctggacttat aaccatgtac
     5641 gtccgggctt ggcagcaggg aaccgtagaa gccggttgcc tgtgccttag acagttcact
     5701 caccgcatcc acaccgttaa taccgatacc gatgatatcg ctcgctttaa agccctgacc
     5761 ttccgtcgcg cgtacgccgc ccagcacggt gctgtcgttc ataccgacaa tcagccaatg
     5821 tttaacttcc ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc
     5881 gttagatttg gtaggtacct gataaatttg tttttccggg aatcctgcag ctttcagcgc
     5941 atccatagac cccgtagtac ggcgacgagc cgtatccagt tcgttggagg taatcgccat
     6001 caccgcgctt tctttgacat cccagccacg tttctgcatc tctttataca gttcctggcc
     6061 ctgacgttcg ccaattttgg tcgcagccat catcaccagc ggaacggtat ccattggctt
     6121 acctttggcg ttaacaaact ggtcatccac ggcaatgact ttcatatcgt agccacgcgc
     6181 tttcgccacg atggcagagc cgagtttcgg gtccggagta caaataacga aacctttcgc
     6241 gccactggca gccaggctgt cgatagcgtt caatgttttt tcgccatccg gcacggcaat
     6301 cttaataact tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg
     6361 gaaccacggc tcttccggtt gcttcaccag gaaaccgagc ttcaggttct ccgccatagc
     6421 ggattgtgac ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat
     6481 ggttctctcc agctttagtg tcgttttgtg tagggcaaaa acgaatgaca ttcgttaaat
     6541 taatcggaaa acaaagcatt accttttaac taaaagataa gtgactgtgt tgacatagtt
     6601 ttagcgagaa attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa
     6661 cgtgcataaa tttagcggga aaagacataa ggaaaagcga atttgtcaga caaattatct
     6721 agtgcacagc agaataatcc ataagattag cctggaagtc cttgttgtct ttggtaccca
     6781 tgcgggatgt tttcttttta accagtcaat aggcctcatt acctggcgtt gagtttttgc
     6841 aatggtgtaa taaccgcaac ttaaagatgt ggaaaatgcg cgtcattcat ttcgccatta
     6901 attatcactg tactcattaa ttaacagaac acgtataatg agagccatat cgcaaaaatg
     6961 aaaaaacgtt ttataaaatc attgcttcat catgaattca aattcatcga ttaatatcaa
     7021 caagatacaa aaagcaccat cattaaaatt cattacagtt acattgattt catcaatgaa
     7081 atgtaaaaat atataaactt gatgatttaa gcattttctt atacccgttc agacgttatt
     7141 cttatttcag atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga
     7201 attattctgc aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga
     7261 tataaggata ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa
     7321 ccgcgagttt tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag
     7381 tctgaacggc accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat
     7441 gcgcatgttt aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt
     7501 tcccggtgaa aaactgaact ctctggaaga actgttccaa aaaacgatgg aagaatacga
     7561 gcaacgttca agcacgctgg cgcagttagc cgatgaagcg aaagaactga atgatgattc
     7621 aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag catgatggcc tgttactgca
     7681 aaccattctt gatgaagtgc gcagtgcgaa acttgctggt atgtgtcctg tgcagaccga
     7741 ccaacatgtt ctgaatgtcg tgtcgcatca gctgcattga tcatacatcg gcgccaatgc
     7801 attgcgccga tgaagttttt gagaaaccgc tgcctcattt ttttgaagca gcggtttttt
     7861 aatgggattc gccctgtggg gtaaacttga gttcaatcaa cgcgatggct ttttggattg
     7921 cccgcatggt gaccgggtct gcggcggcgg gatggttagt aaagtcgata ttcttcagtt
     7981 ggctggacat tttttcgcga acttcaacgg gcgcgatcac atcgagaaca tccagaattt
     8041 gtttgataac caactggcaa gcaaccacat cagaaaccag ttcctgatcg gcattcagcg
     8101 gctgggacat cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc ggcttcacat
     8161 cttcagcgat agtcacatcc accgtcatca gaacacaaaa aaacctgccg gagcaggttt
     8221 tttgttatcg gaacatattg cctggcggta cgtctttgaa cgtcttgcaa tagttattga
     8281 acatactttt caggatcttg cgcagtttca tcgcggtact ccgaccattt gttatacagg
     8341 tgttattgtc tttgcgttta taatatgaca accatcacaa aaatcaatct ttatgtgatg
     8401 caaatcacat aaatacccct ttaatgttat aaaaatgata atcaaaaagc agccccccaa
     8461 tttctgacac atacagatgg caagaaatag cgcctgccag gcgtcttttc cggccattgt
     8521 cgcagcgctg taacgcgtaa aatactgctt tcttttactc ttctggctgg accatgagac
     8581 ttctgattct gactctttcc ttaataacgc tgacaggatg tacggttact cgccaggccc
     8641 atgtgagcga agttgacgcg gcaacaggta ttgttcggtt ggtttatgat caggcttttt
     8701 tgcagcatgc ccgtaccgat cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc
     8761 aggcaggcta tacccacgct atcccctttg gtcagccagt aggcaactgt agcctctttg
     8821 cgggttcctt atgtttgaat acggaattca ctttgtcata tcagtgccat cattccgcct
     8881 tccctgtctt tctctaaacg ttttaatcag cgagggggat cttcgctgat taaataaata
     8941 gcggtaatgc gttttattcc cattcgtatt tttaataaat gatgtttata ttttaccttt
     9001 tgcaaataat aaaataacaa attatagtga cgctacagca acaaatataa cctttgtgga
     9061 gcactatcat gctgaaacca gaaatgattg aaaaacttaa tgagcagatg aacctggaac
     9121 tgtactcttc actgctttat cagcaaatga gcgcctggtg cagctatcat accttcgaag
     9181 gtgctgccgc gttcctgcgc cgtcacgccc aggaagagat gacgcatatg cagcgtctgt
     9241 ttgattatct gactgatacc ggcaacttac cgcgtattaa taccgttgaa tctccgtttg
     9301 ctgaatattc ctcacttgat gaattattcc aggaaaccta taaacacgaa caattaatca
     9361 cgcagaaaat taacgaactg gctcatgctg caatgaccaa tcaggactac ccaacattta
     9421 atttcctgca atggtatgtt tctgagcagc atgaagaaga gaaactgttc aaatcgatta
     9481 ttgataaatt aagcctggca ggcaaaagtg gcgaaggtct gtattttatc gacaaagaac
     9541 tctctaccct cgacacacaa aactaatgct ctccggcggc aggttttctg ccgccgtttc
     9601 aaatcagtga cggcaaatct tactctgatc ggttgaaaaa ccttcttctg aaggtataaa
     9661 tttgcctttt gctgccagaa acgccaccaa ctctcccgca gtcatccctt ctgccgagca
     9721 ggtgtgaaaa cgtgcctgtt caccaaaacg cgctttaatt gctgcttcca gactggcatg
     9781 cgtatattgc tcgcctgatt caatcatcat atttaacact tcatgaccgt gaatagagtc
     9841 catcgttcct cctcaaaaaa agcctagcgt agcgattgcc gcttatgaag actttgcgcc
     9901 agcgcagaac tgaacgcttt ttcttgtaca tttatattta caccatatgt aacgtcggtt
     9961 tgacgaatca gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag
    10021 gacagaagaa agcgtgaaaa acagaactct gggaagtgtt tttatcgtgg cgggaaccac
    10081 aattggcgca ggcatgctgg caatgccgct ggctgcggca ggtgttggtt ttagcgtcac
    10141 gttaatcttg ttaattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga
    10201 ggtgtaccag catgtcccgg cagataccgg tctgggcacg ctggcaaaac gctatctggg
    10261 acgctacggt cagtggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc
    10321 ggcatacatc agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat
    10381 ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg
    10441 tgtcggaact tcgctggtcg atttatttaa ccgttttctg ttcagcgcca aaattatttt
    10501 tttggtggtg atgctggtat tgctgctacc gcatattcac aaagtgaatc ttttaaccct
    10561 gccgttgcaa cagggactgg cactgtctgc aatcccggtg atttttacgt cgtttggttt
    10621 tcacggtagc gtgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg
    10681 ggtgtttata atcggtagtg cgatccccct ggtggcatat attttctggc agttggcgac
    10741 ccttggcagc attgattcaa caacctttat gggattgctg gctaatcatg ccggattaaa
    10801 cgggctgtta caggcgttac gcgaaatggt ggcctctccg catgttgagc tggcagtgca
    10861 tttatttgct gatttagccc tcgccacgtc cttcctcggc gtagcgttag gcttatttga
    10921 ttatctggca gatttgtttc agcgttcaaa taccgttggt ggacgattgc aaactggtgc
    10981 aattaccttt ctgccgccac tggcgtttgc actgttttat ccacgaggat ttgtgatggc
    11041 gctgggttac gccggtgtgg cactggcggt actggcattg attatccctt cgctgttgac
    11101 ctggcaaagc aggaagcaca atcctcaggc gggttaccgg gtgaaaggtg gtcgtccggc
    11161 gctggtggtg gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc
    11221 aggcttgtta ccagaagtgg ggtgattaga ttcttattgg gtgccagaat taacgctgac
    11281 acccaatgtg acctcttaat gcaggcagca ctgcttaaat ttcttaccac tgccacacgg
    11341 acaaggatcg ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc
    11401 ctgcggatgc gccatccagt atgcatgtaa atcaagcgcc gccagtcgaa tggcatctac
    11461 gctctcttca aacgcttctg gcgacatctt ctctacccgc tcgaagtttt cctcagtacc
    11521 gtgcagcgca atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga
    11581 aagtgccacg ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc
    11641 atcgacttct cgcaagccga ataacggctc aaactgctcc ggaaattcgt tcagacgctc
    11701 tgcggtatcg gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga
    11761 cgcccagcgc ggcacatagt cagccccacc ccacacggca accagccact gtgccggttc
    11821 aatctcttgc ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag
    11881 aatggcgtgg tcagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc
    11941 gtttaacggt cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc
    12001 ttacatgccg gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta
    12061 aggagggtgt aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca
    12121 ctgcttattg ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac
    12181 ccgcacagcg cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg
    12241 ggctcgggaa gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat
    12301 ccgctacata accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg
    12361 ctgccgccga tgtcttttaa actggagcgg gaaacgagac tcgaactcgc gaccccgacc
    12421 ttggcaaggt cgtgctctac caactgagct attcccgcat tcatcaagca atcaattaat
    12481 cacttgattt tattatcgtc tggcaatcag tgccgccgtt cgatgcgttg cattctactt
    12541 acctggcgcg atgagtcaac gatatttttc accacttttg atcgtttgct gaaaattgcg
    12601 ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg ctcaaatatt gcaacattga
    12661 ccacagagtc agtaccgcag ccacaaagaa aagtgcaata ccggcgtact caacccaaat
    12721 gttcggacgc cacagcagcc aggctaacgc caccatctgg gcagtggttt tcactttccc
    12781 aatccaggag acagccacgc tactgcgttt acccaactcc gccatccatt cgcgtagcgc
    12841 agaaataata atttcacggg cgatcatcgt tgctgccggt aaggtcaccc accagctgtg
    12901 gtaatgctcg gttaccagca ccatggcgat agccacgaga actttatctg cgacagggtc
    12961 aaggaaagca ccaaaccggg tactctggtt ccagcgacgt gccagaaaac catcgaacca
    13021 gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca aacggcgacc aggtgacagg
    13081 cagataaaag accaatacaa agaatgggat aaggatgaca cggaacagtg taagcaacgt
    13141 agggatatta aattgcataa tgacgggtaa ctatctgttg tcagtaagat tacccctatg
    13201 ttgctacaga gacatcaatg tttcaacgac cagaagatct tttctgccag accttgcgaa
    13261 atacccggca cttttgcaat ttcctcgacg ctggcgttac gtaaaccttg caaaccgccc
    13321 atatatttca acaacatttg ccgacgtttt ggcccgacgc cttcaatggt ttccagggaa
    13381 ctggtatttt tgaccttcgc ccgtttttta cggtgcccgc caatcgcgtg atcgtgtgat
    13441 tcatcgcgaa tatgctggat aacatgcagc gcgggagaat ctggcggcaa actaaatccc
    13501 tcaccttccg gctcaaagaa cagcgtttcc agcccagcct tacgatctgc tcctttggca
    13561 acaccaagta gcagcggatg atttttatcc catgagacat ccagttcggc gaaaacattt
    13621 ttcgcctgcg caagctggcc tttgccgccg tcaataagga tcacatccgg gatcttactg
    13681 tcgtcgatag ctttaccata acgccgacgc agcacctgat tcatcgccgc gtaatcatcg
    13741 cccggcgtga tgccagtaat gttatagcgc cgatactccg cacgcagcgg gccgttagca
    13801 tcaaacacca cacaggaagc gacggtttgt tcacccatgg tatggctgat gtcaaagcac
    13861 tccatccgct tcacttccgg caatttcaac acgctggcaa gcgcggtcag ccgctggtga
    13921 acggtagatt gctgcgaaag tttgctggtt aaggccgtcg ccgcattggt acgcgcgagt
    13981 ttcagataac gcgctctatc gccacgaggt ttggtttgaa cattaatctt gcgtcccgcc
    14041 agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa gattaaaatc gagcaggatc
    14101 tcacccggta aggtgcgcat ctggctgcct tgtaaataga actgacctac gaaggtttcc
    14161 accacctcgc tcagttccgt accgccaggc actttcggga aatagctgcg gctgccgagc
    14221 actttgccct gacgaatgaa caatacgtgg acacaagcca tacccgcatc gaacgccact
    14281 ccaataacgt caaggtcgtc gccggtattg gaaacaaact gtttttcggt gacacgtcgc
    14341 accgcctgaa tttggtcgcg aatccgcgcc gcttcttcaa actccagatt ctggctggca
    14401 gtttccatac gactaatgag ttgcgtaagc acctgatcat ctttgccaga caaaaacagg
    14461 cgcacatact cgacctgctg agcgtattct tcttcactca ccagtccttc aacgcacggc
    14521 cccagacagc gccctatctg atattgcaga cacggacgcg agcggttgcg ataaacactg
    14581 ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg ccagtgtttc acgtacggca
    14641 tagccattcg ggaacgggcc gaaatattca cctttggcat gcttcgctcc acgatgcatc
    14701 gccagacgcg ggtgggtatc gccactcagg aagataaaag gatatgattt atcatcgcgt
    14761 agcaaaacgt tgtaacgcgg ctgataaagt ttgatgtagt tgtgttccag taacagcgct
    14821 tcggtttctg tatgagtaac cgttacatca atttgctgga tctgggcgac cagcgcttcg
    14881 gttttgcgcg aagcgaggtt gctacggaaa tagctggaaa gccgtttttt caggtctttc
    14941 gctttgccga catagataac cgtaccacca gcatcgtaca tgcgataaac gcctggctgg
    15001 ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat cactcactga cttgataatg
    15061 tctccgcatt acacagacca tggcgaattg ccaggtgagt cagctcaaca tcgccatgaa
    15121 tgtttagttt actgaacata cgatagcggt agctgttcac cgttttcgga ctgagattga
    15181 gctgttctga gatctcattg accttctggc ccttggtgat catcagcata atctgcaatt
    15241 cacgttcaga caaactggca aatgggcttt ctgttttttc tggttcgatc tggcttaacg
    15301 ccatttgttg agcgatatca gaagcaatgt aacgctgccc tgaatagaca gaacgaatcg
    15361 cactcacgac ttcctgcgga gccgcgcctt tgctgaggta gcccgcagct ccggcctgca
    15421 tgactttcgc tggtaaaggg ttttctgtat ggacggtaag catgatgatt ttgacatcag
    15481 ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc aatgcccggc atactcatgt
    15541 ccattagcac cacgtcaacg gcatttgccc ggcaccattt aacggcgtct tcaccgcacg
    15601 atgcctcacc gacgactttt atacccttta tatcttccag aatgcgtcgt atccctgcgc
    15661 gcaccagttc gtggtcatca acaagtagaa cgttgatcaa aggaatatct ccagaaatag
    15721 ggataacgct actgatagtt agtcattcgt atattagcgg tttttattgc aactttgaaa
    15781 cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg tcaattcgat tgtccacagt
    15841 tatggtcacg cccgtcacca acggcttccg ggatcgtttc acagtaagcc cggcgatgaa
    15901 atgtgacaaa aattgacttt attcagcaaa aatgaaaatc agcccgctga atatgtaaca
    15961 ttaattaacc cgaacaatac gggcaaaaaa cattcatgat aaaaatattt attggtcatt
    16021 atattaacgt attttatagt actgttgata tcacgctcaa aaaacaacca ctgctatttt
    16081 tagcaaagct tatggtatac tccgccgcct taacattttt caccgcaaat tttcattgca
    16141 acatgacgag gaaaataaat gagtacgcct gatttttcta ctgccgagaa taatcaagaa
    16201 ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc tgatgctgca ggcgatggga
    16261 caagctgacg cgggccgcgt gatgttaaag atggaaaaac agcttgcgct gatcgaagac
    16321 gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc ctaccgtcag
    16381 taatataaaa ccggctgaga gcgtgccttt cagccggttt ttgcatctgg cacgcagaac
    16441 agaaaagaga tcaaattaag ccagtagcgg ccgcgtaaca ggcaacctgc gtcttattcg
    16501 gtgcattgat ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg
    16561 acaaaatcat cgctatctct gctgatgtct taccttccgc cgtccatttc agaatttctt
    16621 tttcgcgctt gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca
    16681 gagccatcag actttcgcgc accagtagct gcatttttaa ttgcagttca tcactaagaa
    16741 tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa gcccaacgcc cgatttggca
    16801 gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc gcgcgcagct tcccataacg
    16861 gctgtgcttc gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag
    16921 ggttcagcac cggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact
    16981 cagggtaatt ggtatagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac
    17041 ataacgaata gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct
    17101 cttctgcggc ctccatcctc tgaaaacgca acaacatcgt gcgaagccag ctgaaaaaat
    17161 ccgtatcctg catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat
    17221 tcaaactatc acataaataa gatttatata taatttatat tattcaggca atgaattact
    17281 tttgcaagcc atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt
    17341 ttatcacgtt tgaaatggcg tcccgccccg actggtgaat cgtcggggct gattttttct
    17401 tattattgca gcagaaactt ctccaggaac tggcgggtgc gaggctgctg aggatcagca
    17461 aataacgctt ttgctacccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg
    17521 tccgcaacat cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc
    17581 tgcgccagct gacgaatggt gttcaggact tcgcccacca gctctggatc cagcgccgac
    17641 gttggctcat caaacaaaat cacttcagga cgcattgcca gagcacgcgc aatcgcaacg
    17701 cgctgctgtt gaccgccaga caaacgacgt ggataactgg tttctttacc ggccagccca
    17761 actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg
    17821 atcaccggcc cttcaataat gttttccagc accgtacgat gcggaaacag attaaagttc
    17881 tggaagacaa acccgacgtg ctgacgcaac tggcgaatca gcgatttttg ctgacttaat
    17941 gaacgtgcag tatcaatagt gatatcgcca acggtgatgg ttcccgcttc cggttgttcc
    18001 agcagattta tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataatagcc
    18061 actacttcgc caggcttcac ctcaaggtcg ataccatgca gcaccgtctg accgtggaat
    18121 tttttcacca ggttcttaac ttcaatggca ctcatttggg ttccctctcc tggcgattaa
    18181 gttgattctc aaaatggttc tgcaacgtcg ataacacagt cgccatgatc cagtagatga
    18241 gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct
    18301 gacggaacag ctccggcacc tggattgtcg cggccagcga cgtatctttt accaggctga
    18361 taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac
    18421 gcatggtctg ccatggcgtc atgccaatac tggctgcagc ttcccactga cctttatcaa
    18481 tagaagaaat ggcggcgcgc agcgtttcgg cagcataggc ggcagtgttc agcgacagac
    18541 caatcattgc tgacggaatg ggatctaatt cgataccaaa ctgtggcagt ccgtaataga
    18601 tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat agagatataa aaacgcgcca
    18661 gccagcgcac gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagcagta
    18721 agccaaaaaa catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga
    18781 acggcaagga atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac
    18841 gtggggatga taggcgaaca gcgcaggcgc accgccggta tgaataaaca aaatcggccc
    18901 ttcatctttg aagcgtttct gactgatacc gtcaatcagc cccgccatcg cttttccggt
    18961 atacacagga tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc
    19021 ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca tcccagagta aaatttccgc
    19081 tgatgcggtc agctccagtt ctttcgcaat cgcctgttgg aggttaacca ctttcggcaa
    19141 ttgatcggca acagaacgcg acacagtcac gccaatcagt tcgctttcag gcatcaggtg
    19201 ttccagccca acagccagtc cggcgtgagt tccggcactg cccgacgcga ccaccaccga
    19261 cgaaatatta actgcccctt cacactgttg cgcaatttcc agcgcactct ccacataacc
    19321 cagcgcgccc aaagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc
    19381 ttcgactcgc gtcgccagct cttccagttg ggcattggga tcggtcagtg cgtcgcacat
    19441 ttcaatctgg gtattgaaca gatccagcaa caaacgattg ccgttggtta aatagttttc
    19501 tgcggttgtg ccaataggat tttccagcag cgccacgcag tgcagaccga gtttcgccgc
    19561 aactgcggca gtctggcgca catggttaga ctggatcgcc ccagcagtaa tcagcgtatc
    19621 ggcaccttca cgcagagcat ctgccgcgag aaattccagc ttacgtaatt tattgccgcc
    19681 cattgccatg ggagtgacgt catcccgttt gatgaaaatt tcccgtccaa gataatcaga
    19741 aaagcgcggc agatattcga gcggcgttgg cgcgccgata aactccagcc gtggaaaacg
    19801 ggttaaatta tgcagtggca taacagcctc cgatgtgtgt tgttgtgatt ttcttattat
    19861 gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa gcgccctttt ttgtcattat
    19921 gctgattatt tggtcacatc agcaccaaac catttttcgg aaagggcttg caaagtgcca
    19981 tctttttgca tttccgcaat tgcatcattc accgctttca gcaggtcttc atttccttta
    20041 cgcagcgcca cgccagactc ctgacgggag aatgcttcac cggttactgc cagcgtatcg
    20101 ttggttttct tcaccagatc cagcgccgcc agacgatcaa cgaggatcgc atcgatacgg
    20161 cctacgcgca gatcctgata tttggtcggg tcatcatcat aggtacgcac atcgacgccc
    20221 tgaacattct gacgcagcca ctcttcatag ttggtgccca gaccgacacc cacttttttg
    20281 cctttcagat catcagccgt tttaatggtg ccttcgttgc cttttttcac cagcgcctga
    20341 ataccagaaa tggtgtacgg ggtagagaaa tcgtattttt tcttgcgctc gtcagaaatg
    20401 gtgacctggt taatcaccac atcaatgcgt ttagagtcca gcgacgccag cataccgtcc
    20461 catttggtcg gttttagtga cgcctcaacg ccaagatgtt ttgccagctg ttgggcaaat
    20521 tccacttcaa aaccggttaa tttgccgtca tccccctgaa aactgaacgg cggataagtt
    20581 ccttccagcc ctaccagcag cgtgccgcgc tctttgactt tattaagcag accttcatct
    20641 gcaaaacttt taacgctcat acccgcaacc agcgccacgg ccatcacacc catcaatgcc
    20701 tgacgtccca gatgtgctaa tttcatattt accccgaatg ttgttatgtc tgtttgcagt
    20761 gtagagccat tgtttgtaaa cacaaaaaca actccgctac atcttattct tatttaatat
    20821 atatcagaag caggttggct ggaggatttc tgcacaagtc cggtacacgt ttgacgctga
    20881 tagtgttgat acttccgtaa cgcgatgcgg taattgttgg tgctggtgga gggcagccac
    20941 ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat cgagggaaat attttgctcg
    21001 tgtagatgat tccccaaacg gcgcagacga acgacatatt cacgcaccgt accagggctc
    21061 atttcagttt gctcaaaaag aaattgctta aagccgataa tatcgaaaaa atcactctgc
    21121 gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg cccatgattg ttgttccact
    21181 tgccagccat tttcatcaag cagcgtgtcc aggcgggaaa tctcgatttt attcactatt
    21241 ttgccgtcgc gaacgagggt aatgcgatcg agtaatttac ggcaatgcgc gcaatgggtc
    21301 tggctgtgtt taaaatcttt aagatagcgg cttaatggcc gtcttttcag gtgctgcacc
    21361 atcattaaga actcccggta gtcaaaatta aagtgcggca tttactgacg ttataactta
    21421 cccagtttag tgcgtaaccg tttaatagcc tggctgtgta actgactgac ccgcgattcc
    21481 ccgacctcca gcaccgcacc aatctctttg agattcagct cttcctggta atagagggtt
    21541 aataccagtt tttcgcgctc tggcaacgtt tcgatagctt ccatcacccg ctggcgcaga
    21601 ttactgtcca gcagttgttg tagcgggttt tctcgctgat gatcatcagt aactagttca
    21661 atgctatcgc cgtgctcttc gcgccactca tcgtaggaga agagctggct gttattggtg
    21721 tcgagcaaca tttggcgata gtcggcaata tcgatcccta aacgttccgc aacctcagtt
    21781 tccgtggcgt tgcggccaag ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc
    21841 gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc gaagttcatc cagcatagcg
    21901 ccacgtatac gctgcactgc gtaagttgta aatgccgttc cttgtagggc gtcatagcgt
    21961 tcgacggcat taagtaaccc aatgccgccc gcctgtagca gatcgtcaag ttccacgctc
    22021 gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc
    22081 cacagcgagt gtttatccat tacaccttca gcggtataga gtgaattcac gataaacagc
    22141 cctgcgttat atgagttatc ggcatgatta tccgtttctg cagggttttt aatcggacga
    22201 ttagtgggtg aaatgagggg ttatttgggg gttaccggta aattgcggac agaaaaaacc
    22261 ccgccgttgg cggggaagca cgttgctggc aaattaccat tcatgttgcc ggatgcggcg
    22321 taaacgcctt atccggccta caaaaatgtg caaattcaat aaattgcaat tccccttgta
    22381 ggcctgataa gcgcagcgca tcaggcaatt tggcgttgcc gtcagtctca gttaatcagg
    22441 ttacgacgat taaccctgca gcagagacag aacctgctgc ggtacctggt tggcttttgc
    22501 cagtacggag ttaccggcct gctggataat ctgcgctttc gacatgttgg acacttcggt
    22561 cgcatagtcg gcgtcctgaa tacgggactg cgcttcagac aggttggtag tggtgttgtt
    22621 caggttggtg acggcggaat ccaaacggtt ttggatagcc cccagggaag aacggaattt
    22681 gtcgatggag ctgatagctt catccagagc tttcagtgga tcttttgtta ctgtgccttc
    22741 gctagttgca tctgttgtta aagtaccatt agcactaaca tatacttcag ccccgacttt
    22801 cggcgcatat ttacccgtac cagttccaga atcaacagtt accttgccgt tgttagcatc
    22861 gattgaatac ttcgtggtgt atgaaccaat tgtagtcatt tcaccatcag catctgtgta
    22921 tgttgctgtt gcattagaaa cgtcagtatt atcggcatat tttgcgctag cagtaccgtc
    22981 acctgcggca aataccgcct gatacgtctg aacgccagat ttataggtaa ttgttgcgga
    23041 tgtatcaccg ttagctgcaa ctgctgtgcc ttgtttagct gtagccactt tatttaaaac
    23101 ggtttctttg ctagctgtat cagtgaacga tacccctgcg ttagcagttg cagcaccagc
    23161 atcagcaccc gttttaaagt tataagttgt tccccccatc gtgactgatg cagtcttatt
    23221 aaacccgatt gattgaccat caccagcttt cttgaataaa ccatcaagcg tagccgcagt
    23281 cgcaccacca gcatcgtttg tagttaagtt accatcacta gttaaatagg cttcctgtcc
    23341 tccgattgtg attttaccat tcgcatcaac atcaaagttc acttcaccgc ttgctgcttt
    23401 ataaacacca ctagcagttt gcccagccgg cgggagaagg ctagctgcta catcacctgc
    23461 ttttgctgaa gtattattcg atacattact gaatgagaag ttaccagcag atgcattgta
    23521 tgtatagacc gtggcagttt gagctgcatc tgctttgata gtaacagtgt caccatcttt
    23581 cagttgatca agggcctttg atgcagtcag cgcattattt gttgtagtta tagtattagt
    23641 tgcagcatcc attttcgctg ctgtcaggtc gctaatggtc gccgctttat tggctatcgt
    23701 accggaacca ttcacgttaa aaccattcag ccccagcgta tcagagtcaa ttttcttcag
    23761 atcaatagtg atagtctggc cgtcgttcgc accaacctga attttcatcg aaccgtcttt
    23821 tgccagtacg ttcacgccgt tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag
    23881 acgggatttg atttcgtcct gaatggagtc cagatccgaa tcagagttag tcccggtaga
    23941 agcctgaacc gtcagttcac gaatacgctg taagttgttg ttaatttcgg acagcgcgcc
    24001 ttcggtggtc tgcgcaacag aaataccatc gttggcgtta cgtgcagcct gagtcaggcc
    24061 tttaatgtta gaagtaaaac ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct
    24121 gttaatacgc aagccagaag acagacgctc gatagaactc gacagcgcag actggttctt
    24181 gttgatatta ttttgagtga tcagcgagag gctgttggta ttaatgactt gtgccatgat
    24241 tcgttatcct atattgcaag tcgttgatta cgttttgggt ttccacccgt cggctcaatc
    24301 gccgtcaacc ctgttatcgt ctgtcgtaaa acaaccttta gaattttttt cacaaacagc
    24361 cattttttgt tagtcgacga aatactcttt tctctgcccc ttattcccgc tattaaaaaa
    24421 aacaattaaa cgtaaacttt gcgcaattca ggccgataac cccggtattc gttttacgtg
    24481 tcgaaagata aaaggaaatc gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct
    24541 ggatttaagt tcaatccttg atagcctcac cgccgcgcaa aaagcgacgc taactcctat
    24601 ttcaaatcag caatcgtcgt ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc
    24661 gctgacgact ttccagaccg ccaatactgc attgtctaaa gccgatcttt ttttcgccac
    24721 cagcaccacc agcagcacca ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg
    24781 gaaatacacc atcagcgtca cccatctggc gcaggcgcaa accctgacaa cgcgcaccac
    24841 cagagacgat acgaaaacgg tgatcgccac cagcgacagt aaactcacca ttcaacaagg
    24901 cggcgacaaa gatccgattg ccattgatat cagcgcggct aactcgtcgt taagcgggat
    24961 tcgtgatgcc atcaacaacg caaaagcagg cgtaagcgca agcatcatta acgtgggtaa
    25021 cggtgaatat cgtctgtcag tcacatcaaa tgacaccggc cttgataatg cgatgacact
    25081 ctcggtcagc ggtgatgatg cgctacaaag ttttatgggc tatgacgcca gtgccagcag
    25141 caacggcatg gaggtctcgg tcgccgccca gaatgcacag ctgacggtca ataacgtcgc
    25201 cattgagaac agcagcaaca ccatcagcga cgcgctggaa aacatcaccc tgaacctgaa
    25261 cgatgtcacc acaggcaacc agacgctgac catcactcag gacacctcca aagcgcaaac
    25321 ggcgattaaa gactgggtga atgcctataa ctcgctaata gataccttca gcagcctgac
    25381 caaatacacc gccgtagatg cgggagctga tagccagaat tctagcaatg gcgcactgct
    25441 cggcgactcc acgctgcgga cgattcagac gcagttgaaa tcgatgctga gtaataccgt
    25501 cagttcttcc aactataaaa cgctggcgca gattggtatc acgaccgatc ccagcgatgg
    25561 caaactggaa ctggatgccg acaaactcac cgctgcattg aaaaaagatg ccagcggcgt
    25621 aggtgcatta attgttggcg atggtaaaaa aaccggcatc acgaccacca tcggcagtaa
    25681 cctgaccagt tggctttcga caacgggcat tattaaagcc gctaccgatg gcgttagtaa
    25741 gaccctgaat aagttaacta aagactacaa cgccgccagc gatcgcattg atgcgcaggt
    25801 cgcgcgctat aaagaacaat ttacccaact ggacgtttta atgacctcgt taaacagcac
    25861 cagcagctac ttaacgcagc agttcgaaaa caacagtaat tccaagtaat caatattcat
    25921 cgggagacag gtcatgtacg cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga
    25981 aagcgccgta atgagcgcca gccaacagca gttggtcacc atgctatttg atggagtgct
    26041 gagcgcactg gttagagcgc gcctgtttat gcaggacaac aatcagccag gcaaaggtgt
    26101 ctctttgtca aaagcgatca acatcattga gaacggactg cgggtgagtc ttgatgaaga
    26161 gagcaaagac gaattaaccc aaaacttaat tgctctttat agctatatgg tcaggcgttt
    26221 gctgcaagcc aatttacgca acgatgtctc cgcagtcgaa gaggtggaag cattaatgcg
    26281 taatattgcc gatgcctgga aagagtcttt actctcccct tctttgattc aggacccagt
    26341 ctgatgaaca atgcaccgca tttgtatttc gcctggcaac aactcgtcga aaaaagccag
    26401 ctcatgttac gcctggcaac ggaagaacaa tgggacgaac tcatcgccag cgaaatggcg
    26461 tatgtgaatg cggtgcagga gatcgcacac ctgactgaag aggttgcccc gtccactacg
    26521 atgcaggagc agctccgccc gatgctgctc ctgattctcg acaacgaaag caaggtaaag
    26581 cagttattac agattcggat ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa
    26641 aaatcggtgt taagtgccta tggcgatcag ggcggctttg tgcttgctcc gcaggataac
    26701 ctcttttgaa tcttaatgag ccgatggctc gcgaataatc cgattacggc tacgcttcta
    26761 atattcccct tgaatggagt cgaagaatgc gtaatcccac gctgttacaa tgttttcact
    26821 ggtattaccc ggaaggcggt aagctctggc ctgaactggc cgagcgcgca gacggtttta
    26881 atgatattgg tatcaatatg gtctggttgc cgcccgccta taaaggcgca tcgggcggct
    26941 attcggtcgg ctacgactcc tatgatttat ttgatctagg tgagtttgat cagaaaggca
    27001 gcatccctac taaatatggt gataaagcac aactgctggc cgccattgac gccctgaaac
    27061 gtaatgacat tgcggtgctg ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa
    27121 aagaagctat tcgcgtgcag cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa
    27181 tcattgagtg tgaaggctgg acgcgttaca ccttccccac tcgtgccggg caatactcgc
    27241 agtttatctg ggatttcaaa tgttttagcg gtatcgacca tatcgaaaac cctgatgaag
    27301 atggcatttt taaaattgtt aacgactata ccggagaagg ctggaacgat caggttgatg
    27361 atgaactcgg taatttcgat tatctgatgg gtgagaatat cgattttcgc aatcatgccg
    27421 tgacggaaga gattaaatac tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct
    27481 ttcgtcttga tgcggtcaaa catattccgg catggtttta taaagagtgg atcgaacacg
    27541 tacaggaagt tgcgccaaag ccgctgttta ttgtggctga atactggtcg catgaagtcg
    27601 ataaactgca aacgtatatt gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc
    27661 tgcagatgaa attccatgaa gcatcgcgca tgggtcgcga ctacgacatg acgcagatct
    27721 tcactgggac gctggtggaa gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg
    27781 acacccaacc gttgcaagcc ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt
    27841 atgccttaat tttgttgcgg gaaaatggcg ttccttcggt gttctatccg gacctctacg
    27901 gtgcacatta cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat atgccaataa
    27961 tcgaacagct tgatgagtta attctcgccc gtcagcgttt cgcccacggt gtacagacgt
    28021 tatttttcga ccatccgaac tgcattgcct ttagccgcag tggcaccgac gaatatcccg
    28081 gctgcgtggt ggtcatgtcg aacggagatg atggcgaaaa aaccattcat ctgggagaga
    28141 attacggcaa taaaacctgg cgtgattttc tggggaaccg gcaagagagc gtagtgacag
    28201 acgaaaacgg cgaagcgacc ttcttttgca acggcggcag cgttagcgtg tgggttatcg
    28261 aagaggtgat ttaaatccat ccccggcggc aagccgggga gatttcatta cggcagtggc
    28321 gtcggtaacg gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg atccacacgc
    28381 tccatcgtca taccatcgta ctcaatggtg ttaccgtcac gctcaacctc gtacagctca
    28441 cgttttacgg taacgttagt gagatcatca gacatcaatg tcaacttacc cggcacggca
    28501 atcacccgct gccactgacg gcaatccagg gtatcgccct ctttagtgac aatgaggctg
    28561 cctatcgctt ccggactgac caacgcacgt tgcggccctt tggtctgcca gtaacccgtc
    28621 agccagtccg gcgcgggggt tttcacgaca ttgttatagt tttcgacttc ggcacagccc
    28681 gccagcaaca tgagcgcacc tgcaattgcg agttttttca tcattcgtcc tgcatgagaa
    28741 gaaaagagga ttgtggcatt aaagccctga tgtcgccagc ctttccaggc tcagagagaa
    28801 atttgatcta accattattt ttgtgaatta ttaccccgaa aataaatttg catggtgtat
    28861 gattcgcgac tcttgtttca aaccttctga gttcagaggc tacattcatg tcatggcagc
    28921 aattcaaaca cgcctggttg attaaattct gggcacccat ccctgcggtc atcgcggcgg
    28981 gtattctctc cacttactat tttggcatca ctggcacctt ttgggcagtc acgggtgaat
    29041 ttacccgttg gggcggtcag cttttgcagc tgtttggcgt ccatgctgaa gagtggggtt
    29101 attttaaaat tatccatctg gaaggatcgc cattaacccg catcgacgga atgatgatcc
    29161 tcggtatgtt tggcggctgt tttgccgcag cgctgtgggc caacaatgtc aaactgcgca
    29221 tgccgcgcag ccgtatccgc attatgcagg ccatcattgg cggtattatc gccggttttg
    29281 gcgcacgtct ggcaatgggc tgtaatctgg cggcgttctt taccggaatt ccacagttct
    29341 cgctgcacgc ctggttcttt gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct
    29401 ttacactgct gcccatcttc cgtattcccg tgaaaatgca gaaagtttct gccgcctcac
    29461 cgctgacgca aaaaccggat caggcgcggc gtcgttttcg tctcgggatg ctggtctttt
    29521 tcggcatgct gggctgggcg ctgctgacgg cgatgaacca gcccaaactg gggctggcaa
    29581 tgctgtttgg cgtcggcttt ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg
    29641 cgttccgcga tatgtggatc accggacgta cccatatggc gaaagcaatc attatcggta
    29701 tggcggtaag tgccatcggg atcttcagtt acgtacagtt aggcgttgaa cccaaaatca
    29761 tgtgggcggg accaaacgcg gtaattggtg gtttactgtt tggttttggc atcgtgctgg
    29821 caggcggctg cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg cactactggt
    29881 gggtcggtct gggcaacgtg atcggctcaa cgattctggc gtattactgg gatgatttcg
    29941 ctccggcgct ggccaccgac tgggacaaaa tcaacctgct gaaaaccttt ggtccgatgg
    30001 gtggcctgct ggtgacatat ttgctgttgt ttgcagctct aatgttgatt atcggctggg
    30061 aaaaacgctt cttccgccgt gcggcaccgc agattgctaa ggagatcgca tgaaaaatat
    30121 cgttcctgat taccgtcttg atatggtggg tgaaccctgc ccttatccgg ctgtcgcaac
    30181 ccttgaggcg atgccgcagt taaaaaaagg ggagatcctg gaagtggtga gcgactgtcc
    30241 gcagtcgatc aataatattc cactggatgc gcgtaatcac ggctatacgg tgctggatat
    30301 tcagcaagac gggccgacca ttcgttattt aattcagaag taatccctca ttcccgctgt
    30361 ggcccacagc gggaacatcg ttacctctct atcacataga ttttacccgc cccctctcct
    30421 attcttttca gtgtgctctc aggaggtttc tatgtgtgga cgctttgccc aatcccaaac
    30481 gcgtgaagat tacctcgcgt ttctcgcgga agatattgaa cgcgatattc cctacgatcc
    30541 cgaacctatt ggcagataca acgtcgcgcc gggaaccaaa gtccttctgc tcagtgaacg
    30601 tgatgaacac cttcatctgg atccggtttt ctggggatat gctcccggat ggtgggataa
    30661 accgccgctg attaacgccc gcgtagaaac tgccgcaacc agtcgcatgt ttaaaccgct
    30721 ctggcaacat ggtcgggtaa tatgctttgc cgatggctgg tttgagtgga aaaaagaagg
    30781 cgacaaaaaa cagccttatt ttatctatcg cgctgatgga caacctgttt ttatagccgc
    30841 gataggtagc acaccgtttg agcgcggtga cgaagccgaa ggagttttaa ttgtcactgc
    30901 agcagcagac cagggcctgg tagatattca tgaccgccgc ccgctggtac tgtcgccaga
    30961 aactgcgcgg gaatggatgc ggcaagacat tggtggtaaa aagaagcctc agaaatcgct
    31021 accagaagct gtgtgccagc aaaccagttc atctggcacc ctgtgtcgcg cgcggtgggt
    31081 aatgttaaaa accagggcgc ggagttaatt caacctattt gataagcctg gagattatct
    31141 acacctggat ggacatcatt gtactatgcc ccgtacatgc cccatcacat cctcaacatc
    31201 aaagtattag agccttgcaa accattatgc gtctcagttt gtctcatcta tcttacgcaa
    31261 aaaaaataga actaatatca agcggcgcta tattgcgtat taaataaaac cacctcaatt
    31321 acattaataa atctaaacaa atatcatgca aataatttcc atggttattt ttaggctatt
    31381 taaattaaaa aaataaatta
//
LOCUS       DAZKHK010000054        31086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKHK010000054 DAZKHK010000000
VERSION     DAZKHK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.054"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(198..503)
                     /locus_tag="ACHOS5_002426"
     CDS             complement(198..503)
                     /locus_tag="ACHOS5_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9777235.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSERN"
     gene            complement(500..895)
                     /locus_tag="ACHOS5_002427"
     CDS             complement(500..895)
                     /locus_tag="ACHOS5_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9777236.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGADWHSVR
                     RCLVDVLACGDLYMSGKYGVFASEQVYRVWRKTPEKTTDQTLIRKLPDGEIRRYDRHQ
                     NIICSECRNSEVMQRVLAFCQGNFQEVME"
     gene            complement(911..1636)
                     /locus_tag="ACHOS5_002428"
     CDS             complement(911..1636)
                     /locus_tag="ACHOS5_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9777237.1"
                     /translation="MESVIDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVTETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHQDIVRQIVDSSSRIVSEK"
     gene            complement(1620..1841)
                     /locus_tag="ACHOS5_002429"
     CDS             complement(1620..1841)
                     /locus_tag="ACHOS5_002429"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9777238.1"
                     /translation="MSKLSEEECDVLEFLLTSGRNTARSVAETLQMSPATVVEILKRL
                     ETEGLVKQLNGFRSAQEERNTGGKNGKCD"
     gene            complement(1834..3939)
                     /locus_tag="ACHOS5_002430"
     CDS             complement(1834..3939)
                     /locus_tag="ACHOS5_002430"
                     /inference="COORDINATES: protein motif:HMM:NF018353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFP9777239.1"
                     /translation="MAEEKTQEYAYAFPVKNEATRKRLGFTHAFYWKSDYSQARVLLR
                     ANVAMLDAGFEPEDFKKPVRVNLPVVSELPAEGVFDTEFCRLYVPGGEDGKTMVYTRA
                     AEAVADREETNSASNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSR
                     EELQALHIALAEELPVVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGF
                     MTAFFEAYLDTDYTDRGLLTKEWCNGNRISRVENKKEAQEKVVEKSPVTGERPRRSEK
                     PTFRTINYEIAAALCPGADCHDLRPVMDSAKKLMADDDERWRAWSTALGVTEGIKNWD
                     RKTIFDMVSLAPSHIATGAAEARREWIENFLIVRGVRDPDWAEPLVCGSVPGHEDNLK
                     RVREAGKRMRKIENSHTTSQSDCDLRKTGAAEVDESKATSSAEIPDADKLLAASRGEY
                     VDGISDPNGQNWVPGIQTRETNAIVQDSACDTSQESEKANQNVETANHSEPDIVTEGP
                     ELQENEPEVQGTEPEAARENGCGGCGKSGGGHCPDCGAVMGDNTYSAVYGEAGEATGD
                     VAERGVVNVQHKVTADEVRKIMKAVKPSVHADTEESTEEMNGELAAPQTDDNDDRSPG
                     ATSEDATTNVFDDSEALLHEEDFRGGDEADVDVRALLDILRQQQVMLEEIRSTQARFT
                     GAVIAMLSSISGGEQVSHE"
     gene            complement(3945..4160)
                     /locus_tag="ACHOS5_002431"
     CDS             complement(3945..4160)
                     /locus_tag="ACHOS5_002431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777240.1"
                     /translation="MDKAQRDAAVAANRHKVSPEQVLIWCEQLKFIVDTANRNTRHNE
                     KSRALEPILAWIEQQKKQAMAEIRKRG"
     gene            complement(4162..4737)
                     /locus_tag="ACHOS5_002432"
     CDS             complement(4162..4737)
                     /locus_tag="ACHOS5_002432"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9777241.1"
                     /translation="MNDLMIDTETTGTSHHSGIISFAAAFFNPDTGDKGDSIYLRLDW
                     VSATRNGAVIDPETFCFWLEQSTETRLEAVRKDECVSERDGTYALINFIEQHADIRLV
                     RVWAKSPVFDCSLIRDMCDRSIGVNASAEMLWPFWNERDVRTVEGLATHLGIPLPYTR
                     KDVTHHALNDVMGQIRNVTTVFAALRGVTEV"
     gene            complement(4748..4903)
                     /locus_tag="ACHOS5_002433"
     CDS             complement(4748..4903)
                     /locus_tag="ACHOS5_002433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777242.1"
                     /translation="MKLFSLRKNKKTEKKKRTYPANNNVRLLRYYQKLQVLSCDPDKK
                     FSLDVWV"
     gene            complement(4999..5256)
                     /locus_tag="ACHOS5_002434"
     CDS             complement(4999..5256)
                     /locus_tag="ACHOS5_002434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777243.1"
                     /translation="MATIQIQKRPKFAIPQLSSDCRRRIHRAQMVTLHSYIQRIADNP
                     CAVDLCIPYLLSYIHDDIEAIDNELISLGLFDQAMGKKKRR"
     gene            complement(5700..5885)
                     /locus_tag="ACHOS5_002435"
     CDS             complement(5700..5885)
                     /locus_tag="ACHOS5_002435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777244.1"
                     /translation="MEKEKLKHFRKGLKDSQRVMAIAVKRLNDGRYESAEELMRGEAA
                     SLFKLADELSDVTEQQS"
     gene            complement(6129..6413)
                     /locus_tag="ACHOS5_002436"
     CDS             complement(6129..6413)
                     /locus_tag="ACHOS5_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP9777245.1"
                     /translation="MKDMLTPIEDRLVQYPVDGVTPAKFGELIGKSTNAVDLMIRNNK
                     LPYVEMRDPDKPNSRAEKIVYIPAYNEGLKKAYYNKPKQLAMAWLEWLGL"
     gene            6538..6846
                     /locus_tag="ACHOS5_002437"
     CDS             6538..6846
                     /locus_tag="ACHOS5_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9777246.1"
                     /translation="MMIYEKLKAMRKAEGLSQQNVCQLLDFSISTLKKYESGAIEPGC
                     AALMKYTTHPRFFKYSLWLMSDQTAEIAGQISPPLSLDGSCSSEGVQDSVTPVTKSRR
                     "
     gene            6931..7914
                     /locus_tag="ACHOS5_002438"
     CDS             6931..7914
                     /locus_tag="ACHOS5_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9777247.1"
                     /translation="MAIKKLDDGRYELDTRTGGRGSKRIRKIFNRKADAVAYERYMLG
                     KLANNEWNTAAKVDRRPLSEILNHWFLCHGQAMKNGEIEKRQLMKTITELNDPAVYEV
                     NKRMLVMHRSRRLASGVSASTINRDIYRLSGMFSALIKLDEFLGDNPVRGLPPLPEKN
                     PGMSFLSSAEINALLDTLKGDYRLVALLCLSTGGRWSEVSTLTPSQLTQQRVIFLETK
                     NGKKRIIPISEELETEVMENASASLFRVDYENFCRKLRKVKPDLPRGQATHILRHTFA
                     SHFMMNGGNIIALQQILGHATIQQTMAYAHLAPDYLQNAVMLNPLKGGIGK"
     gene            complement(8101..8601)
                     /gene="cpxP"
                     /locus_tag="ACHOS5_002439"
     CDS             complement(8101..8601)
                     /gene="cpxP"
                     /locus_tag="ACHOS5_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP9777248.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            8751..9449
                     /gene="cpxR"
                     /locus_tag="ACHOS5_002440"
     CDS             8751..9449
                     /gene="cpxR"
                     /locus_tag="ACHOS5_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP9777249.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            9446..10819
                     /gene="cpxA"
                     /locus_tag="ACHOS5_002441"
     CDS             9446..10819
                     /gene="cpxA"
                     /locus_tag="ACHOS5_002441"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP9777250.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(10925..11599)
                     /gene="yiiM"
                     /locus_tag="ACHOS5_002442"
     CDS             complement(10925..11599)
                     /gene="yiiM"
                     /locus_tag="ACHOS5_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP9777251.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(11748..12731)
                     /gene="kdgT"
                     /locus_tag="ACHOS5_002443"
     CDS             complement(11748..12731)
                     /gene="kdgT"
                     /locus_tag="ACHOS5_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP9777252.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(12991..13611)
                     /gene="sodA"
                     /locus_tag="ACHOS5_002444"
     CDS             complement(12991..13611)
                     /gene="sodA"
                     /locus_tag="ACHOS5_002444"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP9777253.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            13897..14931
                     /gene="rhaT"
                     /locus_tag="ACHOS5_002445"
     CDS             13897..14931
                     /gene="rhaT"
                     /locus_tag="ACHOS5_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP9777254.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(14928..15776)
                     /gene="rhaR"
                     /locus_tag="ACHOS5_002446"
                     /pseudo
     CDS             complement(14928..15776)
                     /gene="rhaR"
                     /locus_tag="ACHOS5_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(15850..16686)
                     /gene="rhaS"
                     /locus_tag="ACHOS5_002447"
     CDS             complement(15850..16686)
                     /gene="rhaS"
                     /locus_tag="ACHOS5_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP9777255.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            16974..18443
                     /gene="rhaB"
                     /locus_tag="ACHOS5_002448"
     CDS             16974..18443
                     /gene="rhaB"
                     /locus_tag="ACHOS5_002448"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9777256.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQFRSTRQTKELCA"
     gene            18440..19699
                     /gene="rhaA"
                     /locus_tag="ACHOS5_002449"
     CDS             18440..19699
                     /gene="rhaA"
                     /locus_tag="ACHOS5_002449"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9777257.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGLLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQITPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLESAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKEILSRRG"
     gene            20250..21074
                     /gene="rhaD"
                     /locus_tag="ACHOS5_002450"
     CDS             20250..21074
                     /gene="rhaD"
                     /locus_tag="ACHOS5_002450"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9777258.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            21084..21398
                     /gene="rhaM"
                     /locus_tag="ACHOS5_002451"
     CDS             21084..21398
                     /gene="rhaM"
                     /locus_tag="ACHOS5_002451"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9777259.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKAHNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(21439..22833)
                     /locus_tag="ACHOS5_002452"
     CDS             complement(21439..22833)
                     /locus_tag="ACHOS5_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9777260.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNTTPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
     gene            complement(23187..23875)
                     /locus_tag="ACHOS5_002453"
                     /pseudo
     CDS             complement(23187..23875)
                     /locus_tag="ACHOS5_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828052.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
     gene            complement(23963..24796)
                     /gene="fdhD"
                     /locus_tag="ACHOS5_002454"
     CDS             complement(23963..24796)
                     /gene="fdhD"
                     /locus_tag="ACHOS5_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP9777261.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            24990..28040
                     /gene="fdnG"
                     /locus_tag="ACHOS5_002455"
     CDS             24990..28040
                     /gene="fdnG"
                     /locus_tag="ACHOS5_002455"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:25575..25577,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9777262.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGIAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            28053..28955
                     /gene="fdoH"
                     /locus_tag="ACHOS5_002456"
     CDS             28053..28955
                     /gene="fdoH"
                     /locus_tag="ACHOS5_002456"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP9777263.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            28952..29587
                     /gene="fdoI"
                     /locus_tag="ACHOS5_002457"
     CDS             28952..29587
                     /gene="fdoI"
                     /locus_tag="ACHOS5_002457"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9777264.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            29584..30513
                     /gene="fdhE"
                     /locus_tag="ACHOS5_002458"
     CDS             29584..30513
                     /gene="fdhE"
                     /locus_tag="ACHOS5_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP9777265.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(30737..30955)
                     /gene="yiiE"
                     /locus_tag="ACHOS5_002459"
     CDS             complement(30737..30955)
                     /gene="yiiE"
                     /locus_tag="ACHOS5_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9777266.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRDEAVGVEELCKQHKE"
BASE COUNT         7558 a         8173 c         7559 g         7796 t
ORIGIN      
        1 aaacctccac cggctattcc cgtttcaata attattttga actttcgcat ttcactacca
       61 ccctgccggg cggtctcctg atgttctgag ggtgcaggaa tccctcctgt taaggattaa
      121 attttattta cagtgctgat tttaattatt cagttctgga ttatgctttc tctttcacct
      181 gccgtagttc ctggtaatta atttcgctca ctggttgcct cctggaaaat aaccgcatgt
      241 cccagtttct ccgccagtgc cagttctgcc ttagcgcctg ccgatcgctg ccagcctttc
      301 agcatataaa ccgcatccac acaacgaatc attgccatgc aaatatccat gtaatgtggc
      361 tgagtcagcc cgtccggaag tactgcggga tttaatacgg tatgcccttc ctgtttcagt
      421 gcttcttccg ccttgtgaaa cgcctcacgg ttgaaatttt catatcccgt catcggaccg
      481 gcgatataaa ttctcaccct cactccatca cctcctgaaa attcccctga cagaacgcca
      541 gtacacgctg catgacttcg ctgtttcggc actcactgca aattatgttc tgatgcctgt
      601 catagcggcg tatttctccg tctggtaact ttcgaatcaa tgtctggtca gttgttttct
      661 ccggtgtctt acgccatacg cgatacacct gttcagatgc aaaaacaccg tatttaccgg
      721 acatgtataa atcgccacaa gccagtacat ccacaaggca acgtctgact gaatgccagt
      781 ctgctcccgt cgctctctcc agttgtgata tcgtcatgcg ttcatttttg cgtaccagcc
      841 cgataattcg ggccttcagt tcttcacgct gttcgtgtgt aaaaggtttc gccataagcg
      901 cctccggcaa tcacttttcc gacacaatac gactggatga atcgacaatc tgtcgaacaa
      961 tatcctggtg cttgttcagc tcccgcagcg cggcgcagac acgctcccac ttctgaacct
     1021 gaccttttgc cctgcgcagc tcgcggttag ccacatgcag cgatggtaaa atcagaccat
     1081 ccggatgttt tctggtgaac gacggctgtg actgcactgt gaccgccaca ctttccgttt
     1141 ttatttcttc ctgtgtttcc gcttcccgga ccggtaacgc aacacctgct ggctgaggaa
     1201 aggctttacc atcggtttcc gttaccgatg cagcttccgg ctctgccggt aaatcagcgc
     1261 ccggtatgca gtaacgaaat ttaccgccct gattcacgcg aatcagacgc cctttgctga
     1321 ttgccatggc cagcgatgaa ttcgcccggc gggaggtaat cccgaacatc agtgccagct
     1381 catccgccgt ttgtgggcca tgttgttcaa tcgcctcagt cagcatttgc gctgtcactt
     1441 tcggtaccgg tgacaccggt tcactttcac cagcctgaat cagccaccac atcgaaccct
     1501 tgttatccgc ttcaccgcgg cgcttcagtt tccacagttc gttgaccgca tcttcacggc
     1561 tgattccaag gcgggccgcc actacctgtg aagaggctct tttcagtgct ttcagtgcat
     1621 caatcacact ttccattttt acctcctgta tttctctctt cctgcgcaga cctgaaacca
     1681 ttcagttgct ttaccagtcc ttctgtttcc agccgtttca atatttcaac caccgtcgcc
     1741 ggtgacatct gcaacgtctc agccacactt cgtgcggtat tgcgtcctga tgtcagcagg
     1801 aattccagta catcgcattc ttcttcactt aacttactca tggctgacct gctcccctcc
     1861 actgatagat gaaagcatgg caataaccgc ccctgtaaaa cgagcctgcg ttgagcggat
     1921 ttcttccagc attacctgct gctgacgcaa aatatccagc aacgctctga catccacatc
     1981 cgcctcatca ccacccctga aatcttcctc atgaagaagc gcttcactgt catcaaaaac
     2041 atttgtggtc gcgtcttctg atgtcgcccc tggtgaacgg tcatcattat catctgtctg
     2101 cggtgctgcc agctcaccgt tcatctcttc cgttgactct tcagtgtcag catgaacaga
     2161 gggtttaacc gctttcatta ttttcctgac ctcgtcagcg gtaactttgt gctgaacatt
     2221 cacaacgccc cgttccgcca catccccggt agcctctccc gcctctccat aaacggcact
     2281 gtatgtgtta tcccccataa ctgcaccgca atccgggcaa tgacctccac cggatttacc
     2341 acatccacca caaccatttt cccgtgctgc ctccggttcc gtgccttgca cttctggttc
     2401 attttcctgc aattccggcc cttccgtcac aatatctggt tcggaatggt tggcagtctc
     2461 cacattctgg tttgcttttt ccgactcctg gctcgtatca caggcagagt cctgaacaat
     2521 ggcgtttgtt tcccgcgtct gtattcccgg aacccaattc tgaccattag ggtcactgat
     2581 cccatcaaca tattcaccac gggaagcagc cagtaattta tcggcatccg gtatttcagc
     2641 agatgacgtc gctttgcttt cgtccacttc agccgctccc gtcttccgca agtcgcagtc
     2701 gctctgcgac gtggtgtgac tgttctcaat cttcctcata cgttttcctg cctcgcgaac
     2761 tcttttcaga ttatcttcat gcccaggcac agaaccacat acaagcggtt ctgcccagtc
     2821 tggatcacga acaccgcgca ctataagaaa gttttcaatc cactccctcc gcgcctctgc
     2881 tgcccctgtt gcgatatgtg acggcgcaag actgaccatg tcaaaaatgg tctttctgtc
     2941 ccagttttta atcccttccg taacaccaag ggctgtcgac catgcacgcc atctttcatc
     3001 atcgtcagcc atcagtttct tcgcggaatc catcaccgga cgtaaatcat ggcagtccgc
     3061 accaggacat aaagcagcag cgatttcata attaatcgtt ctgaaggttg gtttttcact
     3121 gcggcgcggt ctttcccctg tgactggcga tttctccaca actttctcct gcgcttcctt
     3181 tttattttca acgcgggata tgcggtttcc gttacaccac tcttttgtca gcaggcctcg
     3241 atcggtatag tcagtgtcta ggtatgcttc gaaaaaagca gtcatgaacc ccagactgga
     3301 attaacggga ttagggaaaa ctttgtcagt gtcgcgcacc agtttgtgaa ggtcgcgaat
     3361 ctccagcggg tcgagcagct ttgtgttgtg ggaaatagcc agggcagtaa caaccggcag
     3421 ttcttcagct agtgcgatat gtaatgcctg aagttcttcc cgtgaaacgt gcgttaccgg
     3481 tttttcgctg ccatgttgcg caagccagcg aatgggcagt tcctgaccgg aaatcggcag
     3541 gagcatatta tcctcaatct cagccatatc ttcgccgttg acgttagtgt tactggctga
     3601 gttggtttct tccctgtcag ccactgcttc tgcggcgcgg gtatacacca tcgttttacc
     3661 gtcttcacca cccggcacat acaggcgaca aaattccgta tcaaaaacac cttccgccgg
     3721 aagttcgctc actacaggaa gatttacccg cactggcttt ttaaagtcct ccggctcaaa
     3781 ccccgcatcc agcatggcaa catttgcccg aagcagaact ctcgcctgcg aataatcact
     3841 tttccagtaa aaagcatgag taaaccccag gcgctttctg gttgcttcgt tttttaccgg
     3901 aaacgcatat gcatattcct gagttttttc ttctgccatt tttatcaccc ccgttttctg
     3961 atttccgcca ttgcctgttt cttctgctgc tcaatccacg caaggatcgg ctcaagcgcc
     4021 ctgctctttt cgttgtgccg ggtattacgg ttggctgtgt ctacaatgaa cttcagttgt
     4081 tcacaccaga tcagcacctg ttccggcgag accttatgac gattcgcagc tacggctgca
     4141 tcacgttgtg ctttatccac atcacacctc cgtcacaccg cgaagtgctg caaataccgt
     4201 ggtcacattt cttatctgcc ccataacatc attaagggca tgatgggtga catcctttct
     4261 ggtgtaaggc aacgggatac caagatgcgt ggcaagccct tcaaccgttc tcacgtctct
     4321 ctcattccag aacggccaca gcatttctgc actggcattt accccaatac tcctgtcgca
     4381 catgtcacga atcagcgaac agtcaaacac tggtgacttc gcccataccc gcaccagacg
     4441 gatatcagca tgctgctcaa taaagttaat cagcgcatat gtgccatcac gctcagaaac
     4501 acattcatct ttccttactg cttccagtcg cgtttctgtc gattgctcca gccagaaaca
     4561 gaatgtctcc ggatcaatca ctgcgccatt gcgtgtcgca cttacccagt ccagtcggag
     4621 ataaatggaa tctcctttat ctccggtatc aggattaaaa aatgctgccg caaaggaaat
     4681 aatccccgaa tgatgggatg ttcctgtggt ttcagtatca atcatcagat cgttcatagt
     4741 tcccccatta cacccacaca tccaggctga actttttatc cggatcacaa cttaatacct
     4801 gtaatttctg gtaataacgc aacagacgga cgttattatt tgccggataa gtgcgtttct
     4861 ttttttccgt ttttttattt ttacgtaatg aaaacagctt catgtttcca cctcgtcaga
     4921 tatttaagat gtaagactcc cgccgcgtga gcggtgtgtt taatacttcc tgacaacgtt
     4981 aattaatagc gataaaaact atcgtcgttt tttctttccc atcgcctgat caaacagacc
     5041 aagagaaatt aactcattat ctatagcctc aatatcatcg tggatgtaag acaataagta
     5101 cgggatacaa agatcaactg cacagggatt atcagcgatg cgctgaatgt aagaatgcag
     5161 cgtaaccatc tgcgcacggt gtatgcggcg acgacagtca ctactgagtt gggggatcgc
     5221 gaattttggg cgcttttgga tttggattgt agccattgcg gcagcctccg ttgacatgac
     5281 gtactgctat cgcaggaggt ggtatgctca ctggcgatag cccagacggg gataccacta
     5341 ccggcgtcaa cggaaaccgg cccgaccgaa gtcggccccg cctgagctac catagacatg
     5401 gtggtgggtg ttatccagcc ccttccataa gaaaggtttg cgacggcacc agttggataa
     5461 caccgcacca taaaatggcg catcattgcg tatacggtta cgacaacaaa aaagacgctt
     5521 gcggcgtctg atgtcgccgt tgacaagtcc ggggtggtat gcccgactgc cagcactgtg
     5581 gcagcagtga aaaaatacca ccttgccaac acagtattca agctcttttt tatctgtctg
     5641 gtcattttgc acgtaacctc acgctttctt aatcacacga gaccgtaact ttctctggtt
     5701 caggattgtt gttctgttac atcgcttaac tcatctgcca gtttaaacag cgatgccgct
     5761 tctccgcgca taagttcttc tgctgattca taacgaccgt catttaatcg cttcaccgca
     5821 atagccatta cacgctgaga atcttttaat cctttacgga aatgtttcag tttttctttt
     5881 tccatatttt ccgcctcagt tgtttagtgt ttctgtcaaa caaaaaagag gaattccttt
     5941 tccatgattt atctcatcaa aaaatcatta tggtaaagtt atcatggtca gtctgataac
     6001 tcgccacacc aacatgatgt taacgacaac tgatttcttt tcactttaat gatgtgtttg
     6061 tgtctagatt taatattaaa agattcaccc acaccagcca ccagccacca gccaccagcc
     6121 accagttatt acaatccaag ccattccagc catgccattg ccagttgttt tggtttgttg
     6181 taataagcct tttttaaccc ttcgttataa gccgggatgt aaactatttt ttctgctctt
     6241 gaattcggct tatccggatc gcgcatttcc acatatggca gcttgttatt tctgatcatc
     6301 agatccacgg cattagttga tttacctatc aattcaccaa attttgctgg cgtgacgccg
     6361 tcaacaggat attgcaccag gcggtcttct attggagtaa gcatatcctt catatgttat
     6421 tctccgatgt tgcagactcc ttgttactcc tccttacgac ctgagacgag ccacacaatg
     6481 gagcctgata aggaacctct tttattcaaa tttagatcct tataaggaac ttgtcaaatg
     6541 atgatttatg aaaaattgaa agctatgagg aaagctgaag ggctttctca acaaaatgta
     6601 tgtcaacttc ttgatttttc tataagtaca ttaaaaaaat atgagtccgg cgcaattgag
     6661 cctggatgtg ccgcactcat gaaatacacc acccatcccc gattttttaa gtactcatta
     6721 tggctaatgt ccgatcaaac cgcagaaatc gcggggcaga tctccccgcc tctctctctt
     6781 gatggctcct gctcttcgga gggcgttcag gattctgtaa ctcccgtcac aaaatcacgc
     6841 cgctaaggcc ggaaaactgg ttagatctgt attttattta tgctgaatgc ctgtggtcag
     6901 aggcaggacg ctacatcgga gggcttcgct atggcgatta agaagctcga tgatggacgt
     6961 tatgaactgg atacaagaac gggcggacgt ggcagcaaac gcatccgcaa aatatttaac
     7021 aggaaagccg atgctgtcgc ctatgaaaga tatatgctcg gcaaactggc aaacaatgag
     7081 tggaatacag ctgcgaaggt ggatcgccgc ccactcagtg aaatcctgaa tcactggttt
     7141 ttatgtcatg gtcaggcaat gaaaaacggt gagatagaaa aacgacaact gatgaaaaca
     7201 atcacagaac tgaatgatcc tgctgtttat gaggtgaaca aaagaatgct tgttatgcac
     7261 cgaagccgtc gtctcgccag tggtgtaagt gcatcgacaa tcaaccggga tatataccgc
     7321 ttgtcaggga tgttcagcgc actaatcaag ctggatgaat tccttggtga caatccggtt
     7381 cgtggccttc cgccccttcc ggaaaaaaat cccggcatgt catttcttag tagcgctgag
     7441 attaatgccc tgcttgacac actgaaagga gattatcggc ttgtggcatt gctttgcctc
     7501 agtacaggtg ggcgatggag tgaagtgtcc acactaaccc cgtcccagtt gacacagcag
     7561 cgggtgattt ttctcgaaac caagaacgga aaaaaacgaa taatccctat atctgaagaa
     7621 ctggaaaccg aagtaatgga aaatgccagc gcttcgctgt tcagagtgga ctatgaaaac
     7681 ttctgcagaa aattacgcaa agttaaacct gatctgccac gaggacaggc cactcacata
     7741 cttcgccata catttgccag tcactttatg atgaacggag gtaatatcat tgctctgcaa
     7801 caaatcctgg ggcatgcaac aattcagcag acaatggctt acgctcatct tgctccagat
     7861 tatcttcaga atgcagtgat gctaaatccg ttaaaaggag ggattgggaa gtgagttttc
     7921 cgtccacaac ttaccacaca taccgctttc aggacgcctt gagactacct gaggcggaaa
     7981 tgtggacgtt tgtggacggt gataaatgct tgtttaacat gtaacttatt gaaaaaaaag
     8041 acccccacat cacgtggggg aagacaggga tggtgtctat ggcaaggaaa acagggttta
     8101 ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt tttgccattg
     8161 cgtcacgtcg cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa ccgcttgctg
     8221 ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa cctgacgagc
     8281 tatttgctcg ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat caaaattttc
     8341 tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag gctgttcgtg
     8401 ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta aacttatgcc
     8461 gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat gccagttatc
     8521 gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg aggccatgac
     8581 ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga ttcaacgata
     8641 gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac gtaaagtcat
     8701 ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca atgaataaaa
     8761 tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg ctcgagatgg
     8821 aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt ctggacgaca
     8881 gcattgattt acttttgctt gatgtaatga tgccgaagaa aaatggtatc gacacattaa
     8941 aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc ggcagtgaac
     9001 ttgatcgcgt tctcgggctt gagctgggcg cagatgacta tctcccaaaa ccgtttaatg
     9061 atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg agcgagcaac
     9121 agcaaaacaa cgacaacggt tcaccgacgc ttgaagttga tgccttagtg ctgaatccag
     9181 gccgtcagga agccagcttc gacgggcaaa cgctggagtt aaccggcact gagtttaccc
     9241 tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgaacat ttaagccagg
     9301 aagtgctggg caaacgcctg acgcctttcg accgcgctat cgatatgcac atttccaacc
     9361 tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc ttgcgtggtc
     9421 gcggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca tcttcgccat
     9481 cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca agctcgattc
     9541 acgccagatg accgagcttc tggatagcga acagcgtcag ggtctgatga ttgagcagca
     9601 tgtcgaagcg gagctggcga acgatccgcc caatgattta atgtggtggc ggcgtctgtt
     9661 ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga ccaccgaagg
     9721 ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta ttggtcaggc
     9781 cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg tcggtccgtt
     9841 ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg ccagcagttc
     9901 tcaatccgat ttcataaact tactgtttga ccgcccgcta ttactgctga ttgtcaccat
     9961 gctggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac cggcgcgtaa
    10021 gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc cggaactgga
    10081 agcggggcca caggaattcc ttgccgccgg tgccagtttt aaccagatgg tcaccgcgct
    10141 ggagcgcatg atgacctctc agcagcgtct actttctgat atctctcacg aactgcgcac
    10201 cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg gtgaaagcaa
    10261 ggaactggag cgtattgaaa ctgaagcgca acgtctggac agcatgatca acgatctgtt
    10321 ggtgatgtca cgtaatcagc agaaaaatgc gctggttagc gagaccatca aagccaacca
    10381 gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg gcaagtcgtt
    10441 gacggttaac ttcccacctg ggccgtggcc gctgtacggc aacccgaacg ccctggaaag
    10501 tgcgctggaa aacattgttc gtaatgctct gcgttattcc catacgaaga ttgaagtggg
    10561 ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc ctggcgttag
    10621 cccggaagat cgcgaacaga ttttccgtcc attctatcgg accgatgaag cacgcgatcg
    10681 tgaatctggc ggtacaggtt tggggctggc gattgtagaa accgccattc agcagcatcg
    10741 tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga tttggttgcc
    10801 gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt acgccgcatc
    10861 cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc ctacactccg
    10921 ggggttattt tccccacaat cggcgggaaa aatcttcgat cttgccgctc agtcggcgct
    10981 tttgcatcgt tcgcgtccag cttttcgata accctgcggc ggagagtaaa cgatgatact
    11041 gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg gtcacatcgc
    11101 tgacacggga aaccaactcc agtggcgcat cagcagatac caaccctggt gcaattacgc
    11161 tatacaacca ccccacttta cccgtgtttt gcatcaactg cgctatatcg ctgatatcaa
    11221 aatggtaatt gagtttatag caaggtgagc gtggctggct gacctgaatt aatgcctctc
    11281 cccagcggaa aatatcacca atataaacat tactttccgt cagaccgtcg gttgagagat
    11341 tttcgccaaa cgcaggcgca acaaacagct ccgcctgttc cggaaattcc cgcgcccagt
    11401 agagataatg ctcacgagga taatgacaca gcgctctgtc tggcccgccg tggatctttt
    11461 tctccgcctg ttcgtcacct tccagcccca ggtccgtcag catcaactca ccatcaacct
    11521 ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg cctgtgtata
    11581 catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt agaccatatt
    11641 tattttatgt caggcgtgct gttttcgcgg taagtatcca ggcagaaacg caattgaaaa
    11701 ccggacgaac gttagttcat ccggctgtgg ggattaagga agttggttta tttcaccgta
    11761 cctaaaattt cgattttcgc cgctctggct ttgactttac gtgaccagat agaggtaaga
    11821 attggcacca gaatcgaagt cacaatgacc gccgtcgcta ccagcgaagt tgctgccgga
    11881 gccataggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc taccgcggcc
    11941 cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat caatttatcg
    12001 gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag gatccccagc
    12061 aaacctgtct gggcaattac agtcaaatca atggtattgc ccagtgcgaa ggcaaagaat
    12121 ggaatcagcg tctgcaccgc tttgctaaaa aattctcgta actcagggtc aaggttcccc
    12181 agggcaaagc ccaccaggaa cggcaatact gcgccgacga aaacatgcgg ttcaaacgag
    12241 gcaatcccgg cagtgcccag aataatcatc gtcatgagcg gcccggactc caacgacatc
    12301 aacacaaatg ccccagcttc ttcttttgtg ccgtactgct gcatgatgga agcgtaaagt
    12361 ccgccgttgg tcatatccat cgccgccacc agcgccaacg ttgaaagtcc ggcaaaaaat
    12421 ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac cacccacgcg
    12481 acggcaattt tagttaccac cagcgtaccg gatttacgca gtaccgttcc cgtcgcgctt
    12541 aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac cgtaccggta
    12601 atcatcccgt tggtgaatga tccaaaatat ttccccgccc ccggcgagaa ggtgtggcac
    12661 agtgcgccaa ggaatagcgg gacgagcatc atcccccccg ggattttctc aatcgagcgt
    12721 tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt cattcctcaa
    12781 cggagcatca gtgttgatgg aaacgaagat agacattcat ttgatcaata aaagtgatca
    12841 aaaccacatc aattgaaacg ctgttttatt tttatcggat cattgttttt attttgaaat
    12901 tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctgc aatgcggcgt
    12961 aaacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcgaaac gtgccgctgc
    13021 ttcgtcccag ttcaccacgt tccagaactc tttaatgtag tccggacggc ggttctggaa
    13081 tttcagatag taagcatgtt cccacacatc caggcccaga atcgggaagc cggaagcgcc
    13141 agaaatggct tcgcccatca gtggagagtc ctggttagca gtagaaacca ccgccagttt
    13201 atcgcctttt tgcaccagcc atgcccagcc ggaaccaaag cgggaagctg ccgctttttc
    13261 aaattctgct ttgaaattat caacggaacc gaaatcacgt tcgatagccg ctttcaggtc
    13321 gccctgcagg gtggtgcctt ttttcagacc tttccagaac agactgtggt tagcgtgacc
    13381 accagcgtta ttgcgcagta cggttttctt gtctgctggc agctggtcca gtttggtgat
    13441 cagctcttca accggcaggt tggcaaattc tggcaggttt tccagcgccg cgttggcgtt
    13501 gttgacgtaa gtctgatggt gtttggtgtg gtggatttcc atggtctgct tatcgaagtg
    13561 cggttccagg gcatcgtaag catacggcag ggatggcagg gtataactca tattcatctc
    13621 cagtgttgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat tatagttaat
    13681 taaatgatat tgaaaatgat tatcaatgcc gtacttttca taagggtatg gttttgcagg
    13741 aaaatgccca agatgtgaag caaatcaccc acttaatgcc gtgattgcca gtaaatcggc
    13801 agcggcggca acaggcgaaa ggttaatcga cagctcaatt tttacactca tctcgtcgga
    13861 gatgtgacgc gacgaaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    13921 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    13981 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatc
    14041 ctgccgtggg ccatcagcgc cctgttacta ccgaatttct gggcgtatta cagctcgttt
    14101 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    14161 aactacggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    14221 attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgctg
    14281 attaataccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgta
    14341 gggattgtaa ctcgcgccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    14401 ttcaatctga aaaaagggct ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg
    14461 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcgct gggcgtcgat
    14521 cccctgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc aatcatcaac
    14581 ctcggcttct gcttcattcg tctggcaaaa gtgaaagatt tgtcgctaaa agccgacttc
    14641 tcgctggcaa aaccgctgat cattcacaat gtgctactct cagctctggg tgggttgatg
    14701 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    14761 tacatcagtt ggatgctgca tatgagcttc tatgtattgt gcggcggtat cgttgggctg
    14821 gtgctgaaag agtggaacaa tgcaggacgc cgtccggtaa cggtgttgag cctcggttgt
    14881 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    14941 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    15001 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    15061 tgctggagga gatattgcgc atgacacact ctgacctgtc gcagatattg attgatggtc
    15121 attccagtct gctgacgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    15181 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccagccgggt aatcagctta
    15241 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    15301 agcaacatca ccaactgccc gaacagcaac tcagccattt cgttagcaaa cgacacatgc
    15361 tgactacttt catgctcaag ctgaccgata acctgccgcg cctgcgccat ccccacgcta
    15421 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc tccctgccag
    15481 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    15541 acggaagcgt aggagtgttt atcgtcagca tgaatataaa agagatcgcc acgggtaatg
    15601 cgataagggc gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca
    15661 caaaaatcat gtgtatgttc ggcaaagaca tcttgcggat aacggtcagc cacagcgact
    15721 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactaatg cgccaccgtg
    15781 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    15841 agattcgcgt tattgcagaa aaccatcccg tccctgacga atatcacgcg gtgaccagtt
    15901 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccgc agcgataggc
    15961 gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag
    16021 gcggttcagg taacgctgag gcgtcagccc cgtttgctgc ttaagctggc gatgcagcgt
    16081 acgcaatgaa agagaaaatt gatccgccac agcgtcccaa ttcacctcat cggcaaaatg
    16141 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg
    16201 caaactgctt ttacgcagca agagcagtaa ttgcataaac agaatttcac gactggcggt
    16261 cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg
    16321 ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcct gctcttgtgg
    16381 cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagtacatt
    16441 ggtcagacac agattatcgg tatgttcata cagatgccga tcatgatcgc gtacgaaaca
    16501 gaccgtccca ccggtgatgg tatagggctg cccattaaat acatgaatac ccgtgccatg
    16561 ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag
    16621 ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac
    16681 ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcatga ggtcaggttc
    16741 ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt
    16801 gaattttcag aaaatgcggt gagcatcaca tcaccacaat gaagcaaatt gtgaacatca
    16861 tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtca
    16921 cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct
    16981 ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt
    17041 acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatggcctgc
    17101 atagccagaa cggttatgtc acctggaatg tggatagcct ggaaagtgcc attcgccttg
    17161 gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg
    17221 gcgtggattt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc
    17281 gcgatagccg caccaatggc ctaatggcgc aggcacaaca acaactcggc aaacgcgata
    17341 tttatcaacg tagcggcatc cagtttctgc ccttcaatac gatctatcag ctacgtgcac
    17401 tgacagagca gcaaccagaa cttattccac acattgctca cgctttgctg ataccggatt
    17461 actttagtta tcgcctgacc ggcaagatga actgggaata taccaacgcc acgaccacgc
    17521 aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg agcggtgcca
    17581 acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cataggtcac tggatttgcc
    17641 cgcagggtaa tgagattccg gtggtcgccg ttgccagcca tgataccgcc agcgcggtta
    17701 tcgcctcgcc gttaaacggt tcacgcgccg cttatctctc ttctggcacc tggtcattga
    17761 tgggcttcga aagccagacg ccatttacca atgacacggc gctggcagcc aatatcacca
    17821 acgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc ttatggctgc
    17881 ttcagcgagt gcttcaggag cggcaaatca acgatcttcc ggcgcttatc gccgcaacac
    17941 aggcacttcc ggcctgccgc ttcatcatca atcccaatga cgatcgcttt attaatcctg
    18001 acgagatgtg cagcgaaatt caggctgcgt gtcgggaaac ggcgcaaccg atcccggaaa
    18061 gtgatgctga actggcgcgc tgtattttcg acagtctggc gctgctgtat gccgatgtgt
    18121 tgcatgagct ggcgcagctg cgtggtgaag atttctcgca actgcatatt gtcggcggcg
    18181 gctgccagaa cacgctgctc aaccagctgt gtgccgatgc ctgcggtatt cgggtgatcg
    18241 cagggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg
    18301 aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac taccgcgaat ctgaccacct
    18361 ttacccctaa tcctgacagt gaaattgccc actatgtggc gcaatttcgc tccacacgac
    18421 agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca
    18481 gcgtttcgcg gcagtaggga ttgatgtcga ggaggcactg cgccaacttg atcgtttacc
    18541 cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttt
    18601 gctgaccggt gggattcagg ctactggtaa ttatccgggc aaagcgcgta atgccagtga
    18661 gctacgtgcc gatctggaac aggctatgcg gctgattcca gggccgaaac ggcttaattt
    18721 acatgccatc tatctggaat cagacacgcc agtctcgcgc gaccagatca caccagagca
    18781 cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc
    18841 ctgtttttcg catccgctaa gcgccgatgg ctttacgctt tcccatgccg acgaccgcat
    18901 tcgccagttc tggattgacc actgtaaagc cagccgccgt gtttcagcct attttggcga
    18961 gcaactcggc acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac
    19021 cgttgaccgt ctcgccccac gccagcgttt gctggcagca ctggatgaag tgatcagcga
    19081 gaagctaaac cctgcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc
    19141 ggagagctac acggttggct ccaatgagtt ttacctgggg tatgccacca gccgccagac
    19201 ggcgctgtgc ctggacgccg ggcatttcca cccgactgaa gtgatttccg acaagatttc
    19261 cgccgccatg ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg ttcgctggga
    19321 cagcgatcac gtagtgctgc tggatgatga aacccaggcg attgccagtg agattgttcg
    19381 tcacgatctg tttgaccggg tgcatatcgg cctcgacttc ttcgatgcct ctatcaaccg
    19441 cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctac gtgcgttact
    19501 ggaacctacc gctgaactac gcaagctaga atcggcgggc gattacactg cgcgtctggc
    19561 gctactggaa gagcagaaat cgttgccgtg gcaggcggta tgggaaatgt attgtcagcg
    19621 tcacgatacg ccagcgggta gcgaatggct ggagaacgtg cggacttatg agaaagaaat
    19681 tttgagtcgc cgcgggtaaa cactgccgga tgcggcacaa gtcttatccg gcctacgggt
    19741 cggcaatagt tgtaggcctg ataagacgcg acagcgtcgc atcaggcatt gattgccgga
    19801 tgcggcacaa gtgccttatc cggcctacgg gtcggcaata gttgtaggcc tgataagacg
    19861 cgacagcgtc gcatcaggca ttgattgccg gatgcggcac gagcgccata tccggcctac
    19921 gggtcggcaa tagttgtagg cctgataaga cgcgacagcg tcgcatcagg cattgattgc
    19981 cggatgcggc acgagtgcct tatccggcct acgggtcagc aacagttgta ggcctgataa
    20041 gacgcgacag cgtcgcatca ggcattgatt gccggatgcg gcacgagtgc cttatccggc
    20101 ctacgggtca gcaacagttg taggcctgat aagacgcgac agcgtcgcat caggcattga
    20161 ttgccggatg cggcacaagt gccttatccg gcctacgggt cgtacatccg gcaacaggca
    20221 cggacgtcca attaacagga acacagaaca tgcaaaacat aacccagtcc tggtttgtcc
    20281 agggaatgat caaagccacc accgatgcct ggctgaaagg ctgggacgag cgcaacggcg
    20341 gcaacctgac gctgcgcctg gatgacgccg atatcgcacc atatcacgac aattttcacc
    20401 aacaaccgcg ctatatcccg ctcagccagc ccatgccttt actggcaaat acaccgttta
    20461 ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct tgatcctgcg gctaacttag
    20521 gcatcgtaaa agtcgacagc gacggcgcgg gctaccacat tctttggggg ttaaccaacg
    20581 aagccgtccc cacttccgaa cttccggctc acttcctttc ccactgcgag cgcattaaag
    20641 ccaccaacgg caaagatcgg gtgatcatgc actgccacgc caccaacctg atcgctctca
    20701 cctacgtgct ggaaaacgac acagcggtct tcactcgcca actgtgggaa ggcagcaccg
    20761 agtgtctggt ggtattcccg gatggcgttg gcattctgcc gtggatggta cccggcacgg
    20821 acgaaatcgg ccaggcaact gcacaagaga tgcaaaaaca ttcgctggtg ttgtggccct
    20881 tccacggtgt gttcggttcc ggaccgacac tggatgaaac cttcggttta atcgacaccg
    20941 cagaaaaatc agcacaaata ttagtgaagg tttattcgat gggcggcatg aaacagacca
    21001 tcagccgtga agagttgata gcgctcggcc agcgtttcgg cgttacacca ctcgccagtg
    21061 cgctggcgct gtaaggagca aacatgatcc gcaaagcctt tgtcatgcag gtaaaccccg
    21121 acgcccacga agagtatcag cgtcggcata atcccatctg gccggaactg gaagccgtgc
    21181 tgaaatctca cggtgcgcat aactacgcca tctatctcga caaagcgcat aatctgctat
    21241 ttgccacggt agagattgaa tctgaagaac ggtggaatgc ggttgccagt acagatgttt
    21301 gccaacgttg gtggaaatat atgaccgacg ttatgcccgc taatgcggat aacagcccag
    21361 tcagtagcga actgcaagaa gtgttttacc tgccttaatg agttgcggtg ccggaaaata
    21421 tccagcaccg ccctttcatc agaagaagta tttaaagcga acgcgtgcgc cgtaacggtc
    21481 atcactatcg tcgccgtgta tcttatcgcc gtcgctatcc agaatggagt aatacgtccc
    21541 gagataaata ttaaagttct ccatatccat cacattagcg aactgataag acgcatgaat
    21601 ggtgtgaatg ttgtatgttc cttcatcatc aatccagcaa tcgttatcac aaaccacgcc
    21661 actaaattca tcaatcttat tatgtgcata gatataaccc agctcgaaac gtttccacag
    21721 cgcgttaatc cctgccgtga aatctttttc attattagca tctaaatagg cggtattaag
    21781 attaaccacg atgccatttt ccggatcggt tttcaggcca ttccaggtca tactcatgcc
    21841 ataaccggta cgatcggact gatcgacaaa attacccttg ctatcggtat aaccataagc
    21901 attatttacc acattcgctt ccatcgctgc ggaaacggtg aattcttccg tcggcgacca
    21961 ggcaattacc ggacgcagat aagcaacatt tttctgctgt tccatatcgc gtccatgata
    22021 attaccgtcg ttatataaag atgttccgtc ttccagtaac gtgtttaatt caaaatacca
    22081 gttatcgagt tgtttgctga cgaggaaatt accgccagcg ttagaacgtc cgcggccctc
    22141 tttcatcata tagatataac cgctgccatc gtcataaaga tcgttcgcag tattaccgga
    22201 atgttcaaca aaggtatcct gattcagtgg gaacatatcg taggcttcaa aacggcctac
    22261 tttgaccttc cagtcgtttt ctttgccaaa gaagaaaacc gcatcatcga tatttactga
    22321 gccgtgcata tcccccagcg gttgtgcgga gaacccagcg aaatagccat tatccagttt
    22381 tcgcatacca tcaaaaccta acagaatacg accattgaga ttccactgtt cattagtggg
    22441 atcgctattc acatcagcgt tggtcattgc cagcaggcca tgattacttt ccgcatccat
    22501 attaaattct acgtcaccgt agatttttaa atcgccatac cctgaaagca ggagattagg
    22561 cggggtggtg tttttcttct ctgactccgt cgttgattga ccgtttaccg gcgtgttgcc
    22621 ctgtgcggtt ttaatttcgc ggatctcggc ggcttgttgt gtctgaagct gtttaacctg
    22681 cccctctgct tttgctgccc gcatttctgc cgcttccagg cgttcttcca gcaatgccat
    22741 gcgttgttcc agcgttaaat gctgtgtttt tgccataccg gtactacatg ccaataataa
    22801 accgatggct aaagataatg tcgatttttt cattatttac gttccctgaa ataaaggaga
    22861 ccctacgctt tccaaaagga atacgtatat agagaaatat aaggtgtgca ctttcgaatt
    22921 tttaatccaa cttcagcagg ccgatggata gtatcccgcc gcataatatg agtaaatccg
    22981 ctgatttcct ggaacgtgag gaatataaca atagtgccgt ttttaactaa atatcgtaaa
    23041 aaatattaga tatgattcac gctgactcat aagccacaaa tataaataac gcttaaacaa
    23101 attatctgag acatttaaac attcacaaaa tcattaaata atgatacttt attcacaaaa
    23161 tttatccttc actctgcttt atattgttac gacttcgtga catatccgtg ctcatcacaa
    23221 acccgccccg caattacaat taactgaaat atttcatcga ccatttcgtt gtaatcgcat
    23281 aacgctcgtg gaaatgagtc ttcggccatc cagcttgagt ttgcgtcctg aagttgctct
    23341 ttatcccagt cagagtacgg gactttatct ctgatgcggc gaacgtattt tttagcctcg
    23401 agttgatact ggcctgccga attaataaac gagaaattca tgccgctttt atccgcttct
    23461 ttggcttgcg ccaccagcgt ttccagttca gacactttct tcattgccgc ttctgcatct
    23521 aacttatctt gcgatatcag gttattaatt tgtttcgctg aaatcatgac tgccagagag
    23581 tagtagtgga atgttttccc ttctctttct tcaatatttt tcaattccgt aagctggcgc
    23641 ttgtcattaa tttcctgaat cgccgcatga taagattctg ctacaggttc aaaggcttcc
    23701 agatttttaa gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg tctttataat
    23761 tttcctgggt gtaatattta tccatttcgt taatggtatt acccaacgct acggcagcat
    23821 caatataact caccgccacg ccatcaatag gttgcagcgc aggcgttaac gccactacac
    23881 gcgttttgtt tatgaaaaaa taacaaaatt cacgaaatga ataagtcctg taatttaata
    23941 tatatcgcag gacttatttt aattaattgc tcaaacgctg cggatgggta taaaccgttg
    24001 cccgacccgg tttacaaaag cccaccagcg tcagattaca gcgctcagcc acttctacag
    24061 caagtgtggt cgcggcagac accgcaaaca aaatctctac gccgcacatc gccgactttt
    24121 gcaccatttc ataactggca cggctggaaa ccagcaccgc accttgctgc cagctttccc
    24181 cttcttgtga ccggcggcct aacagtttgt ccagcgccac atggcgaccc acgtcttcat
    24241 gcccgccgac cagttcgcca gatggcaaca tccatgcagc ggcgtgagta caaccagtca
    24301 gttgccccac tggctggaaa tcattgagat gacgtaatgc atcatccagt ttgttgagat
    24361 caaacgtctg ggtgaacggc agcggctgca ccggtttacc gatatcgtta agctgttcta
    24421 caccgcatac gccacagccc gtacgtcccg ccagcgcccg gcggcgttcc ttcaacccca
    24481 taaagcggcg gctggaaagc tcaatttgta cttccaggcc attacaggaa ggaacgacat
    24541 ccatgccgaa gatatcgcgc gggctttcga taattccttc ggaaagcgaa aaaccgagcg
    24601 caaagtactc aaggtctttg ggcgacgcca tcatcaccac gtgcgaaatg ccgttgtaga
    24661 ccagcgcaac gggaacttct tccgccacct catctaaccg tgggtgttgt aaatcatcac
    24721 gccgccataa ctcaatttga cgaacacctg tgatatttgt cacattctcg atttcttttt
    24781 gctgtgtttt tttcactttc tattaaccag ataagaacac acacaccaac attatggtat
    24841 tctgttacaa atccttcctg gatggaggga aattgagcca attctggacc tttgcggccc
    24901 cttccgcaaa gaaaaataac tcccactccc tgcacacgca gcaagcgaat gtaaatggga
    24961 cgtgacaatg tcgaaacaag gagcaatcca tgcaggtcag cagaaggcag ttctttaaga
    25021 tctgcgctgg cggtatggca ggcaccacgg cagcggcact gggttttgca cccagcgtag
    25081 cactcgcgga aacccggcag tataaactgc tgcgcacccg cgaaacccgt aatacctgca
    25141 cctattgttc cgtaggctgt gggctgttga tgtacagcct cggtgacgga gcaaaaaacg
    25201 ccaaagcatc tatcttccat atcgaaggtg acccggatca cccggtcaac cgcggagcac
    25261 tttgtccgaa aggcgctggc ctggtggatt tcatccactc cgaaagccgc ctgaagtttc
    25321 cggaataccg tgcgccaggt tctgataaat ggcagcaaat cagttgggaa gaggcgtttg
    25381 atcgcatcgc caaactgatg aaagaagacc gcgatgcgaa ctacattgcg caaaacgccg
    25441 aaggcgtgac tgttaaccgc tggctctcca ccgggatgct gtgtgcttcc gcgtcgagca
    25501 acgaaaccgg ctatttaacg caaaaattct cccgcgcgct gggtatgctc gcggtcgaca
    25561 accaggcgcg tgtctgacac ggaccaacgg tagcaagtct tgctccaaca tttggtcgcg
    25621 gtgcgatgac caaccactgg gtcgacatca agaatgccaa cctcgtcgtg gtgatgggcg
    25681 gtaacgccgc tgaagctcac ccggtcgggt tccgctgggc gatggaagcc aaaattcaca
    25741 acggcgcgaa gctgattgtg atcgatcctc gctttacacg tacggcggcg gtggctgact
    25801 actatgcccc tatccgttcc ggtactgaca ttgctttcct gtcaggcgta ttgctgtacc
    25861 tgctgaacaa tgaaaaattc aaccgcgaat acaccgaagc ctataccaac gccagcctga
    25921 tcgtgcgtga ggattacggc tttgaagatg gcctgttcac cggctacgac gcggaaaaac
    25981 gcaagtacga taagtccacc tggacttacg aactggacga aaacggcttc gccaaacgcg
    26041 atactaccct gcaacatcca cgttgcgtgt ggaacttgct gaaacagcac gtttcccgct
    26101 acacgccaga tgtggttgaa aacatctgtg gtacgccaaa agacgcattc ctgaaagtct
    26161 gcgaatacat cgctgagacc agtgcccacg ataaaactgc ctcgttcctt tacgcgctcg
    26221 gctggacgca acactccatt ggtgcgcaaa acattcgtac gatggcgatg atccagttgc
    26281 tgctcggcaa tatggggatg gctggcggcg gcgttaacgc cctgcgcggt cactccaata
    26341 ttcaggggct gaccgacctg gggctactgt cgcagagtct gccaggttac atgacgttgc
    26401 caagcgagaa gcagaccgat ctgcaaactt acctcgctgc caacacgcca aaaccactgc
    26461 tggaaggcca ggtcaactac tggggcaact acccgaaatt cttcgtctca atgatgaagg
    26521 ccttctttgg tgataaagcg acggcggaaa atagctgggg ctttgactgg ttgccgaagt
    26581 gggataaagg ctacgacgtc ctgcaatact tcgagatgat gaaagagggc aaggtcaatg
    26641 gctatatctg ccagggcttt aaccctgttg cctcattccc gaacaaaaac aaagtgatcg
    26701 gctgtctgtc gaagctgaaa ttcctcgtga ctatcgaccc gctgaacact gaaacctcta
    26761 acttctggca gaaccacggt gagctgaacg aagttgactc gtcgaagatc cagaccgaag
    26821 tgttccgtct gccatcgacc tgcttcgcgg aagagaacgg ttcaatcgtt aactctggtc
    26881 gctggttgca atggcactgg aaaggtgcgg acgccccggg gattgcgctg actgatggcg
    26941 agatcctctc cggtatcttc ctgcgcttgc gcaagatgta tgccgaacag ggtggcgcga
    27001 acccggacca ggtgctgaac atgacctgga actacgccat tccgcatgag ccatcttcag
    27061 aagaagtggc aatggagagc aacggcaaag cgctggccga tattaccgac ccggcaaccg
    27121 gtgcggttat cgtcaagaaa ggccaacaac ttagctcgtt cgcccaactg cgcgatgacg
    27181 gtacaacctc ctgtggctgc tggattttcg ccggtagctg gacgccggaa ggcaaccaga
    27241 tggcgcgtcg tgataatgcc gatccgtctg gcctcggtaa cacgctgggc tgggcatggg
    27301 catggccgct taaccgccgc attctgtata accgcgcctc cgcagatccg cagggtaacc
    27361 cgtgggatcc gaagcgtcag ttgctgaaat gggacggcac taagtggacc ggctgggata
    27421 ttccggatta cagcgcagcg cctccgggca gcggcgtagg gccgtttatc atgcagcagg
    27481 aaggcatggg acgtctgttt gcccttgata agatggcgga aggtccgttc ccggaacact
    27541 acgagccgtt tgaaacgccg ctgggaacta acccgctgca tccaaacgtt atctcgaacc
    27601 cggctgcgcg aatctttaaa gacgacgccg aagcattggg taaagccgat aagttcccgt
    27661 atgtcggaac cacctatcgt ctgaccgagc acttccacta ctggaccaaa cacgcgctgt
    27721 tgaatgcgat tttgcaacca gagcagtttg tggaaatcgg ggagtcgctg gcgaataaac
    27781 ttggcattgc ccagggcgat actgtgaaag tctcctccaa ccgtggctat atcaaagcca
    27841 aagcggtagt gaccaaacgt attcgcacgc tgaaagcaaa cggcaaagat atcgatacca
    27901 tcggtattcc gattcactgg ggctatgaag gtattgcgaa aaaaggcttt attgccaata
    27961 cgttgacgcc attcgtcggt gatgcgaaca cgcagacgcc ggagtttaag tccttccttg
    28021 tgaatgtgga aaaggtgtaa cggagacgac atatggctta tcaatcgcaa gatatcattc
    28081 gtcgttccgc gactaacggt ctgacccccg cgcctcaggc gcgggacttc caggaagaag
    28141 tggcgaaact catcgacgtt accacctgta tcggctgtaa agcctgtcag gtggcgtgtt
    28201 cagagtggaa cgacattcgc gataccgtcg gcaataacat tggggtgtac gacaatccca
    28261 atgatttaag tgccaaatcg tggacggtga tgcgattctc ggaagtggag cagaacgaca
    28321 aactggaatg gctgatccgc aaagacggct gtatgcactg ttccgatcca ggctgcctga
    28381 aagcgtgccc ggcggaaggg gcaattattc agtatgccaa cggtatcgtc gacttccagt
    28441 ccgagcagtg catcggctgc ggttattgca ttgcgggctg tccgttcgac attccgcgcc
    28501 tcaacccgga agacaaccgc gtctacaaat gtacgctgtg cgttgaccgc gtggtggttg
    28561 ggcaagaacc ggcctgcgtg aagacctgcc caacgggcgc gattcacttc ggtacgaaag
    28621 agtcgatgaa aacgctggcg agcgagcgcg tggcggagct gaaaacccgt ggttacgaca
    28681 atgcgggttt gtacgacccg gcaggcgtcg gtggtacaca tgttatgtac gtgctgcacc
    28741 atgctgacaa gccaaatctg tatcatggct tgccggagaa cccggaaatc agcgaaaccg
    28801 tgaaattctg gaaaggcatc tggaaaccgc tcgcagctgt tggctttgcg gctaccttcg
    28861 cagccagtat cttccactac gtgggtgtcg gtccgaaccg tgcggatgag gaagagaata
    28921 atctgcacga agagaaagac gaggagcgca aatgaaacga cgtgacacca tcgtgcgcta
    28981 caccgcgccg gaacgtatca accactggat caccgccttc tgcttcatcc tggcggcggt
    29041 gagcgggctg ggctttttgt tcccgtcctt caactggttg atgcaaatca tgggcacacc
    29101 gcagctggcg cgcattctgc acccgtttgt cggcgtggtt atgtttgcct cgttcatcat
    29161 catgtttttc cgttactggc atcacaacct aatcaatcgg gatgatatct tttgggcgaa
    29221 gaatattcgt aagatcgtcg tcaacgagga agtaggtgac accgggcgtt ataacttcgg
    29281 tcagaaatgc gttttctggg cggcgattat tttcctggtc ctgttgctgg tgagcggcgt
    29341 gattatctgg cgtccttatt ttgcgcctgc tttctcaatc ccggtgatcc gattcgcgtt
    29401 aatgctgcat tcatttgccg cagtggcgtt aattgtggtt atcatggtgc atatctacgc
    29461 cgccctttgg gtgaaaggca cgattaccgc catggtggaa ggatgggtga ccagcgcatg
    29521 ggcaaagaaa catcacccgc gctggtaccg tgaggtccgc aagacaacgg aaaagaaagc
    29581 tgaatgagta ttcgcataat cccgcaagat gagctgggtt cgagcgagaa acgtacggcg
    29641 gatatgattc cgccgttatt gttccctcgg ctcaagaatt tatacaaccg ccgcgccgag
    29701 cgtctgcgcg agctggcaga aaataatccg ctgggtgatt acctgcgctt tgctgcgctt
    29761 atcgcccacg cccaggaagt ggtgctgtac gaccatccgc tggagatgga tctgaccacg
    29821 cgcattaaag aagccagcgc acaaggcaag cccccgctgg atattcacgt tctgccgcgt
    29881 gataagcact ggcaaaagct gctgatggcg ctgattgctg aactgaaacc tgaaatgagc
    29941 ggccccgcgc tagcagtgat tgagaatctg gagaaggcat cgactcagga gctggaagat
    30001 atggccagcg cactgtttgc ctctgatttc tcgtccgtca gcagcgataa agcgccgttt
    30061 atctgggctg cgctgtcgct ctactgggcg cagatggcta atctgatccc cggcaaagcc
    30121 cgcgctgaat acggcgaaca acgtcaatat tgcccggtat gtggctctat gccggtgtcc
    30181 agcatggtgc aaattggcac cactcagggt ctgcgttacc tgcactgcaa cctgtgtgaa
    30241 actgaatggc acgtagtacg cgtaaaatgc agcaactgtg aacagagcgg caaactgcat
    30301 tactggtcgc tggatgacga acaggccgcg attaaagccg aaagctgcga tgactgcggc
    30361 acttacctga agattcttta tcaggaaaaa gaaccgaaag ttgaagccgt ggcagatgac
    30421 ctcgcctctc tggtactgga cgcgcgaatg gagcaagaag gctatgcccg cagttccatc
    30481 aacccgttcc tgtttccggg tgaaggggag taatctctga cgttttccgg gtggcgctgg
    30541 tcgcccggat tacagctcat agggtgtgac gacacctgct attcccgcaa aatctctcgt
    30601 atttcgcgag gcgttttcca gatatgatgt aacagttgca gttattataa gttcaagtaa
    30661 aacaaccttg tattaatacc agaataaggt catctggttt tttgttttta tattcaagaa
    30721 tgaaaaaatc tttttgtcat tccttatgct gcttacatag ttcttcaaca ccaactgcct
    30781 cgtccctttg cctgccgcct tttgccttac gcaccggaaa ttgctgccgc tccagatatt
    30841 gctctaccgc ttcgcgtaaa attgcagatc gtgaaacctt acgccagccc ctgagtttat
    30901 tcagccgttt aattgcctct tctgataaat gaagaaaaac cgtattcatc gccattacca
    30961 ccaccttaca tatattactc attaatgtat gtgcgaaaat tacgccggaa agttaattaa
    31021 cgaaagcgga ttttttcata ttgttttgtt tttgcgagac gctttccaga aataataaga
    31081 acgttg
//
LOCUS       DAZKHK010000055        30881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKHK010000055 DAZKHK010000000
VERSION     DAZKHK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.055"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            138..680
                     /gene="zapC"
                     /locus_tag="ACHOS5_002460"
     CDS             138..680
                     /gene="zapC"
                     /locus_tag="ACHOS5_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP9777267.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(677..1786)
                     /gene="ycbX"
                     /locus_tag="ACHOS5_002461"
     CDS             complement(677..1786)
                     /gene="ycbX"
                     /locus_tag="ACHOS5_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP9777268.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKLAR"
     gene            1884..3992
                     /gene="rlmKL"
                     /locus_tag="ACHOS5_002462"
     CDS             1884..3992
                     /gene="rlmKL"
                     /locus_tag="ACHOS5_002462"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP9777269.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWTQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVNDVAQLANPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4004..5911
                     /gene="uup"
                     /locus_tag="ACHOS5_002463"
     CDS             4004..5911
                     /gene="uup"
                     /locus_tag="ACHOS5_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9777270.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELELLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6041..7294
                     /gene="pqiA"
                     /locus_tag="ACHOS5_002464"
     CDS             6041..7294
                     /gene="pqiA"
                     /locus_tag="ACHOS5_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP9777271.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPSVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7299..8939
                     /gene="pqiB"
                     /locus_tag="ACHOS5_002465"
     CDS             7299..8939
                     /gene="pqiB"
                     /locus_tag="ACHOS5_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP9777272.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKGKKDPEPKRAKQ"
     gene            8936..9499
                     /gene="pqiC"
                     /locus_tag="ACHOS5_002466"
     CDS             8936..9499
                     /gene="pqiC"
                     /locus_tag="ACHOS5_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP9777273.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9755..9922
                     /gene="rmf"
                     /locus_tag="ACHOS5_002467"
     CDS             9755..9922
                     /gene="rmf"
                     /locus_tag="ACHOS5_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP9777274.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(9992..10510)
                     /gene="fabA"
                     /locus_tag="ACHOS5_002468"
     CDS             complement(9992..10510)
                     /gene="fabA"
                     /locus_tag="ACHOS5_002468"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP9777275.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(10579..12339)
                     /gene="ycbZ"
                     /locus_tag="ACHOS5_002469"
     CDS             complement(10579..12339)
                     /gene="ycbZ"
                     /locus_tag="ACHOS5_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9777276.1"
                     /translation="MTITKLAWRDLVPDTDSYQDIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSTDDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12525..12977
                     /gene="matP"
                     /locus_tag="ACHOS5_002470"
     CDS             12525..12977
                     /gene="matP"
                     /locus_tag="ACHOS5_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP9777277.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13055..14095)
                     /gene="ompA"
                     /locus_tag="ACHOS5_002471"
     CDS             complement(13055..14095)
                     /gene="ompA"
                     /locus_tag="ACHOS5_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP9777278.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNFDGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14451..14960)
                     /gene="sulA"
                     /locus_tag="ACHOS5_002472"
     CDS             complement(14451..14960)
                     /gene="sulA"
                     /locus_tag="ACHOS5_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP9777279.1"
                     /translation="MYTSGYAHRSSSFSSTASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15179..15808
                     /gene="sxy"
                     /locus_tag="ACHOS5_002473"
     CDS             15179..15808
                     /gene="sxy"
                     /locus_tag="ACHOS5_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP9777280.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAPYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNIRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15771..17933)
                     /gene="yccS"
                     /locus_tag="ACHOS5_002474"
     CDS             complement(15771..17933)
                     /gene="yccS"
                     /locus_tag="ACHOS5_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP9777281.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(17943..18389)
                     /gene="yccF"
                     /locus_tag="ACHOS5_002475"
     CDS             complement(17943..18389)
                     /gene="yccF"
                     /locus_tag="ACHOS5_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9777282.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            18512..20566
                     /gene="helD"
                     /locus_tag="ACHOS5_002476"
     CDS             18512..20566
                     /gene="helD"
                     /locus_tag="ACHOS5_002476"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP9777283.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQAQAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMHERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20598..21056)
                     /gene="mgsA"
                     /locus_tag="ACHOS5_002477"
     CDS             complement(20598..21056)
                     /gene="mgsA"
                     /locus_tag="ACHOS5_002477"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9777284.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21152..21814)
                     /gene="yccT"
                     /locus_tag="ACHOS5_002478"
     CDS             complement(21152..21814)
                     /gene="yccT"
                     /locus_tag="ACHOS5_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP9777285.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            21987..22400
                     /gene="yccU"
                     /locus_tag="ACHOS5_002479"
     CDS             21987..22400
                     /gene="yccU"
                     /locus_tag="ACHOS5_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9777286.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22445..22762)
                     /gene="hspQ"
                     /locus_tag="ACHOS5_002480"
     CDS             complement(22445..22762)
                     /gene="hspQ"
                     /locus_tag="ACHOS5_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP9777287.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22820..24010)
                     /gene="rlmI"
                     /locus_tag="ACHOS5_002481"
     CDS             complement(22820..24010)
                     /gene="rlmI"
                     /locus_tag="ACHOS5_002481"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP9777288.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24105..24383
                     /gene="yccX"
                     /locus_tag="ACHOS5_002482"
     CDS             24105..24383
                     /gene="yccX"
                     /locus_tag="ACHOS5_002482"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9777289.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGKLTDFRIR"
     gene            complement(24380..24709)
                     /gene="tusE"
                     /locus_tag="ACHOS5_002483"
     CDS             complement(24380..24709)
                     /gene="tusE"
                     /locus_tag="ACHOS5_002483"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP9777290.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGITLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24800..25459)
                     /gene="yccA"
                     /locus_tag="ACHOS5_002484"
     CDS             complement(24800..25459)
                     /gene="yccA"
                     /locus_tag="ACHOS5_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP9777291.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25869..26888)
                     /locus_tag="ACHOS5_002485"
     CDS             complement(25869..26888)
                     /locus_tag="ACHOS5_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9777292.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26866..27108)
                     /locus_tag="ACHOS5_002486"
     CDS             complement(26866..27108)
                     /locus_tag="ACHOS5_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9777293.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27176..29614)
                     /locus_tag="ACHOS5_002487"
     CDS             complement(27176..29614)
                     /locus_tag="ACHOS5_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9777294.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRFGIKGGFYWSTEKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQEKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLD
                     VEAAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINDGLA
                     AECKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEV
                     NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQLETEQND
                     QKAEQNSQNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQET
                     FDEKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHEITS
                     TSRTCDHLMIDLETMGKNPNAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SAIWQKLIPSQADF"
     gene            complement(29707..29895)
                     /locus_tag="ACHOS5_002488"
     CDS             complement(29707..29895)
                     /locus_tag="ACHOS5_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9777295.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(29892..30080)
                     /gene="dicB"
                     /locus_tag="ACHOS5_002489"
     CDS             complement(29892..30080)
                     /gene="dicB"
                     /locus_tag="ACHOS5_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9777296.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(30566..30718)
                     /locus_tag="ACHOS5_002490"
     CDS             complement(30566..30718)
                     /locus_tag="ACHOS5_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9777297.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
BASE COUNT         7781 a         7470 c         7930 g         7700 t
ORIGIN      
        1 gctttatttt cagccctggt tgttttatat tcatcgctgt tgcttattta gacattttgt
       61 acttttattg ttgaggttat taagcgaagc gacaatggat tgtgtcgctg cggttttata
      121 aggttgggga gtggcagatg cgaattaaac cagacgataa ctggcgttgg tattacgatg
      181 aagagcacga tcgaatgatg ctcgatttag ccaatggtat gctatttcgc tcacgttttg
      241 cgcgcaaaat gttgactcca gatgcctttt ctcccgcagg tttttgcgtt gatgacgccg
      301 cgctctattt ctcttttgaa gaaaagtgcc gcgattttaa tttatctaaa gaacaaaaag
      361 ccgaactggt gctgaatgca ctggtagcaa ttcgttatct caaaccgcaa atgccgaaaa
      421 gctggcattt tgtatctcat ggtgaaatgt ggatgcccat gccgggcgat gcggcctacg
      481 tttggttgag cgacactcat gagcaagtga atttactggt cgttgaatct ggcgagaatg
      541 ccgcgctatg tctactggca caaccctgcg ttgttattgc gggtcgcgcg atgcagttgg
      601 gcgacgccat taaaatcatg aacgacaggc tgaaaccgca ggttaatgtt gacagcttca
      661 gcctcgaaca ggcagtctaa cgcgccagct taagcgcagt cttcggtaca caactacagc
      721 aaagaatagt gccatcatcg cctattgctg atttttttag cggcgtgact tcgccttcta
      781 aaagctgaac acggcaactt ccacaaatgc ccgcgcggca agaataaggg atacgaattc
      841 cctgattttc taattgctcc agcagcacct gttggttatt tccacgaaat gcctgtccct
      901 gccagtcaat atctacgttt gcgtccggtt gttgcgtgat gttggcagta tcatcagcgg
      961 cacctgcgcc gtaaatttta gccggagccg ttgccagaat ttccacctcg tcgccaacgc
     1021 gaatcacgcc gctattacgg gcaattaaat tctgaccaaa atcgacatcg ccgttatcct
     1081 gggcagtacg gaaagattgc agcgttttta atggttcgcc tgccggatgt ttttgccctt
     1141 tttctgggct gacggtggtg aaaatacagc ggctacaagg cttaaccaca tcaaacacga
     1201 catcaccaat gcgaatcact ttccagctat cttcttccca ggccgaagca ccggaaacca
     1261 ccagattggg gcggaattgc tccattttta cactggccgg acaacgttgt tggagatcac
     1321 gtaacgaggc ctcgttagca agaaggtaag gatagccatc agcaaatgac aggggtacag
     1381 tgttgtggcg tttcacgcgc cgggtcattt gtggccccac ccagcgtaat tgcacttcgc
     1441 gggagaaaaa tccacttagc catttgttga tcgcgtctgg cgcaattcgc gcggtaaaat
     1501 gtgtgcccca aacttcggtt ggtgcgtctt gtgtggcgaa atcagcaaaa cgaacatatg
     1561 cactactgcc atctggtgcg gtgagatgca agccatcatg cacgggtgaa ggagtaaacc
     1621 gtaccattag cggaaactgg cgagcggtaa taaacgtacc gtcaggttcc gtgatcataa
     1681 agatgcgatc gaaggccaga ccactgacat ctgccagggt atgtgtaaga ccaatgccgc
     1741 gcatcgattt aacaggatga ataaaaagcc gggttaatgt cgccacggtg cggtcctcaa
     1801 atgaaaataa gccctcaact ttatgacatg acgcgcttat tagctataat gcgcaacaat
     1861 tttcttagag taaaagtgac aatatgaatt ctctgtttgc cagtacggcc cgtgggctgg
     1921 aagagctgtt aaaaactgaa ctggaaaacc tgggggccgt tgaatgccag gtggttcagg
     1981 gtggggtcca tttcaagggc gacacacggc ttgtttacca gagcctgatg tggagccgcc
     2041 tggcctcgcg tattatgttg ccgctgggcg agtgtaaggt ttacagcgat ttagacctct
     2101 atctcggtgt tcaggcgatc aactggacag agatgtttaa tcctggcgcg accttcgctg
     2161 tccacttcag tggtttgaat gacaccatac gcaacagtca gtacggtgcg atgaaagtga
     2221 aagacgcgat cgtcgatgct ttcacgcgga aaaatctgcc gcgtccaaat gttgatcgcg
     2281 atgcgccgga tatccgcgtt aacgtctggc tgcataaaga aaccgccagt atcgcccttg
     2341 atctcagtgg tgatggttta catctgcgtg gctatcgcga tcgtgctggc attgcgccga
     2401 tcaaagaaac cctggcagcc gctattgtga tgcgatccgg ctggcagcca ggaacaccgc
     2461 tgctcgatcc aatgtgtggt tcaggtacgt tgctgattga agcggctatg ttggcgaccg
     2521 accgcgcacc gggcttgcac cgtgggcgtt ggggctttag cggctggacg cagcatgatg
     2581 aagctatctg gcaggaagtg aaagcggaag cgcaaactcg tgcccgtaaa ggcctggcgg
     2641 agtatagctc ccatttctac ggttcggaca gtgacgcacg ggtgattcaa cgtgcgcgca
     2701 ctaacgcccg tcttgcgggg attggtgaac tgatcacctt tgaggtgaac gatgtcgcgc
     2761 aactggccaa tccgctgccg aaagggccgt acggtacagt gttgagcaac ccgccatacg
     2821 gtgaacgtct ggacagcgaa ccggcgctga ttgcgctgca tagtctgctg ggccgaatca
     2881 tgaaaaacca gttcggtggc tggaatctct ctttgtttag tgcctcgccg gatctgctaa
     2941 gctgcctgca actgcgtgca gacaaacagt acaaggcgaa aaacggcccg ctggactgcg
     3001 tacagaaaaa ctaccatgtt gccgaaagca caccagacag caaaccggca atggcagcgg
     3061 aggactacgc caaccgtctg cgtaagaacc tcaaaaaatt cgagaagtgg gctcgccagg
     3121 aaggaattga atgttaccgc ctgtatgacg ccgatctgcc ggaatataac gttgctgttg
     3181 accgttatgc cgactgggtg gtggtgcagg agtatgcgcc gccaaaaact attgatgcgc
     3241 acaaagcgcg tcagcgtctg ttcgatatta tcgctgcaac catttcggta ctggggattg
     3301 cgccaaacaa actggtgctg aaaacccgtg aacgccagaa gggcaaaaac cagtaccaga
     3361 aactgggcga gaagggcgag tttctggaag ttaccgaata taacgctcac ttgtgggtga
     3421 acctgacgga ttacctcgat accggtctgt tcctcgatca ccgtatcgcc cgtcgtatgc
     3481 ttggtcagat gagcaaaggc aaagatttcc tcaacctgtt ctcttatacc ggcagcgcca
     3541 ccgtgcatgc gggattaggc ggtgcacgca gcaccaccac cgtggatatg tcgcgtactt
     3601 atctggagtg ggcagaacgc aacctgcgtc tgaatggctt aactggccgt gcgcatcgcc
     3661 tgattcaggc cgattgcctg gcgtggctgc gtgaggcaaa tgaacagttc gatctgatct
     3721 ttatcgatcc gccaaccttt tctaactcaa aacgaatgga agatgcgttt gatgttcagc
     3781 gcgatcatct ggcgctgatg aaagatttga aacgtctgct gcgtgcaggt gggacgatca
     3841 tgttctcgaa caacaaacgc ggtttccgta tggatctcga cggtctggcg aaactgggac
     3901 tgaaagcaca agaaattacg caaaaaacgc tctcccagga tttcgcccgt aaccgccaga
     3961 tccacaactg ctggctgatt accgcagcct gaaaggaata gtaatgtcat taatcagtat
     4021 gcatggcgca tggctgtcgt tcagcgacgc gccgcttctc gataacgcag aactgcatat
     4081 cgaagataac gaacgtgttt gtctggtggg gcgtaacggc gcaggcaaat cgacgttaat
     4141 gaagatcctc aaccgtgaac aggggctgga tgacggtcgc attatttatg agcaagattt
     4201 gattgtagct cgtctgcaac aggatccgcc gcgtaacgtt gagggtagcg tttatgattt
     4261 cgttgccgaa ggcattgaag aacaagcgga atacctgaaa cgctatcacg atatttcgcg
     4321 cctggtgatg aacgacccga gcgagaaaaa tctcaacgaa ctggcgaagg ttcaggaaca
     4381 gctggatcac cacaacctgt ggcagctgga aaaccgcatc aacgaagtgc tggcgcaact
     4441 aggattagat cctaatgttg cgctgtcgtc gctttccggc ggctggttgc gtaaagcggc
     4501 attaggacgc gcgctggtga gcaatccgcg cgtattgctg ctcgacgaac cgactaacca
     4561 cctggatatt gaaaccatcg actggctgga agggttcctc aaaaccttca atgggacaat
     4621 tattttcatt tcccacgacc gttcgtttat ccgcaatatg gcgacgcgca ttgttgatct
     4681 cgatcgcggc aagctggtga cctatccagg gaattacgac cagtacctgc tggaaaaaga
     4741 agaagccctg cgcgtggaag aattacaaaa tgccgagttc gatcgcaaac tggcgcagga
     4801 agaggtgtgg atccgccagg ggatcaaagc acgtcgtacc cgtaatgaag gccgcgtacg
     4861 cgccctgaaa gcaatgcgtc gtgagcgtgg tgaacgtcgc gaagtaatgg gtaccgcaaa
     4921 gatgcaggtg gaagaagcca gccgctccgg taagatcgtt ttcgaaatgg aggacgtttg
     4981 ctaccaggtt gacggtaagc aactggtgaa agatttttct gcccaggttc tacgcggcga
     5041 caaaattgcc ctgattggtc cgaatgggtg cggcaaaacc acgctgctga aactaatgct
     5101 cggtcagctt caagcagaca gcgggcgtat tcacgttggc accaaactgg aagtggctta
     5161 tttcgatcag caccgcgcgg aactggatcc cgataaaacg gtgatggata accttgccga
     5221 aggtaagcaa gaggtgatgg ttaacggcaa gccacgccac gtattgggct atttgcagga
     5281 cttcctgttc catccaaaac gggcgatgac gccggtacgt gcgctttctg gcggtgagcg
     5341 taaccgcttg ttgttggcgc gtttgttcct caaaccaagc aacttattga ttcttgacga
     5401 accgaccaac gatcttgatg tcgaaacgct ggaactgctg gaagaactga tcgacagcta
     5461 tcagggcacg gtattgctgg ttagccacga tcgtcagttt gtcgataaca ccgttacaga
     5521 atgctggatc ttcgaaggcg gcggtaaaat tggtcgttat gtcggcggtt atcatgatgc
     5581 ccgtggtcag caagaacagt atgtggcgct caaacagcct gcggtgaaaa aaagcgaaga
     5641 agccgccgcg ccaaaagcag aaactgtaaa acgcagcagt agcaaactaa gctataaatt
     5701 gcagcgtgaa ctggagctgc taccgcaatt gcttgaagat ctggaggcga agctggaagc
     5761 tttacagacg caagtggcgg atgcttcctt cttcagtcag ccgcatgagc agacgcaaaa
     5821 agtgctcgcc gatatggctg ctgcagagca ggagctggag caagcctttg aacgctggga
     5881 gtatcttgaa gcgttaaaaa atggtggctg atcgcaaaat agtcgattta aacagagact
     5941 gctgacaacg gtaaaagtgc ctgatgtact gggtttatca ggcatgaaaa ccaacacttt
     6001 gttagtcatc tgcaaaatag cgcatcatta aggagtacca atgtgcgaac atcatcatgc
     6061 cgcgaagcac atcctgtgct cgcagtgtga catgctggtg gcgttaccgc gccttgagca
     6121 tggtcagaaa gcggcatgtc cccggtgtgg cacaacgtta accgtggcgt gggatgcccc
     6181 tcggcagcgt cccaccgcct atgcgttggc tgcactgttc atgctgttgc tgtccaactt
     6241 gtttcctttt gtgaatatga acgttgcagg agttaccagt gaaattacat tactggaaat
     6301 tcccagcgtg cttttttctg aggactacgc cagcctcggc acctttttcc tgttgtttgt
     6361 gcaactggtt cccgcgtttt gtctgataac cattctgtta ctggtgaatc gcgcggaatt
     6421 accggtccgt ttaaaagagc aactggcacg ggtgcttttt caactcaaaa cctggggaat
     6481 ggcggagatt ttcctcgcgg gtgtgctggt cagtttcgtt aaactgatgg cttacggcag
     6541 cattggggta ggcagcagct ttctcccctg gtgtttattt tgtgtcctgc aactgcgcgc
     6601 ttttcagtgc gttgatcgtc gctggttatg ggacgacatc gccccgatgc cagaactgcg
     6661 ccagccgcta aaaccaggtg tcacggggat acgtcagggg ctgcgttcgt gctcctgttg
     6721 tacggcaatc cttcctgctg atgaacccgt gtgcccgcgt tgtagtacca aagggtacgt
     6781 tcggcgtaga aacagcctgc agtggacact cgcgctgctt gtaacgtcca tcatgctgta
     6841 tcttccggct aatattttgc ccatcatggt gacggattta ttaggctcga agatgccgtc
     6901 gacgattctc gctggggtca ttctgttatg gagcgaaggc tcttatcccg tcgctgcggt
     6961 gatttttctg gccagtatta tggtgccaac gttaaagatg atcgccattg cgtggctgtg
     7021 ttgggatgcc aaaggacacg gcaagcgcga cagtgaaaga atgcatttga tttatgaagt
     7081 tgttgagttt gtaggccgct ggtcgatgat tgacgttttc gttatcgcgg tgctctcggc
     7141 gctggtgcgt atgggaggtt taatgagtat ttatccggca atgggtgcat taatgtttgc
     7201 tttagtcgtc ataatgacaa tgttttctgc tatgacgttt gacccgcgtt tgtcgtggga
     7261 tcgtcaacct gaatcagagc atgaggagtc ctgacagtat ggaatctaat aatggggaag
     7321 ccaaaatcca gaaagtgaag aactggtctc ccgtgtggat atttcctatc gtcacggcgc
     7381 tcattggggc ctgggttctt ttttatcatt acagccatca gggaccggaa gtgaccctga
     7441 tcaccgcgaa tgcggaagga attgaaggtg gcaaaaccac cattaaaagc cgtagcgttg
     7501 acgtcggcgt ggttgaaagc gccacactgg ctgatgattt gacgcacgtt gaaatcaaag
     7561 cgcggctgaa ttccggtatg gaaaaattgc tgcataaaga taccgtcttt tgggtggtga
     7621 aaccgcagat tggtcgagaa gggattagcg gcctgggaac gctactgtct ggggtttata
     7681 tcgaactgca gccaggcgcg aaaggcagca aaatggataa atacgatttg ttggactcgc
     7741 caccgttggc cccgcctgat gcgaaaggta tccgtgtgct tctcgatagc aaaaaagccg
     7801 ggcagctctc gccaggagat ccggtgctgt tccgtggcta tcgggtaggt tcggttgaaa
     7861 ccagcacttt cgatacgcaa aaacgcaata tcagctatca actgttcatc aatgcacctt
     7921 atgaccgact ggtgaccagc aatgttcgtt tctggaaaga tagtggcatt gcggttgatc
     7981 tgacgtcagc gggaatgcgt gtggagatgg gctcattgac aacgctgctt agtggcggtg
     8041 tcagctttga tgtgccggaa ggtctggatt tagggcagcc agtggcaccg aaaacagctt
     8101 tcgttttgta tgatgatcag aagagcattc aggattcgtt gtacaccgat cacattgatt
     8161 atctgatgtt ctttaaagat tcggtacgcg gtctgcaacc gggagctccg gtagaattcc
     8221 ggggtattcg cctgggtacc gtaagcaaag tgccattctt tgcgccgaat atgcgtcaga
     8281 catttaacga tgattaccgt attccggtac tgattcgtat cgagccagag cggctgaaaa
     8341 tgcagcttgg cgaaaatgcg gatgttgttg agcaccttgg cgaattgttg aaacgtggtt
     8401 tacgcggatc gctgaaaacc ggaaacctgg tcactggcgc actgtatgtt gatctcgatt
     8461 tctatccaaa tacgcctgca ataaccggta ttcgtgaatt taatggttat cagattatcc
     8521 cgaccgtcag cggcggcctg gcgcaaatcc agcaacgact gatggaagcg ttggataaga
     8581 tcaacaaact gccactgaat ccgatgattg aacaggcaac cagtacgctt tctgaaagtc
     8641 agcgcacaat gaaaaacctg caaacgacgc tggatagcat gaacaagatc cttgccagcc
     8701 agtcgatgca gcagttaccg acggatatgc agtcaacgtt gcgtgaattg aatcgcagca
     8761 tgcagggctt ccagcctggt tccgcagcct acaacaagat ggtggcggat atgcagcgcc
     8821 ttgatcaggt gttgcgagaa ctgcaaccgg tgctgaaaac gctcaacgag aagagtaacg
     8881 cgctggtatt tgaagcgaag ggcaaaaaag atccagagcc gaagagggcg aaacaatgaa
     8941 aaagtggcta gtgacgattg cggcactgtg gctggccgga tgcagctccg gcgaaattaa
     9001 taaaaactat taccagttac ctgtggtgca gagcggtaca caaagcaccg ccagccaggg
     9061 taatcgcctg ttatgggttg agcaggtcgc tgttcctgac tatctggcgg ggaatggtgt
     9121 ggtttatcaa accagtgatg tgaagtatgt gattgccaac aacaacctgt gggccagccc
     9181 gttggatcaa cagttgcgca acaccctggt tgccaacctg agtacgcaac tacccggctg
     9241 ggtggttgcc tcccagcctc tgggaagcgc ccaggacacg ctcaatgtta ccgtcacgga
     9301 gtttaacggt cgctatgatg gcaaggtcat tgtcagtggt gagtggctgt tgaaccacca
     9361 gggacaactg atcaaacgtc cgttccgtct ggaaggagtg caaactcagg atggttacga
     9421 tgagatggtt aaagtgctgg ccagtgtctg gagtcaggaa gccgcttcta ttgcgcaaga
     9481 gataaagcgt ctaccttaat cataaagatt tgtaaatata accgtctccg gtatgttgcc
     9541 tgaggcggtt tttttgtttc taacgtgcgg aaaaagttgt tcctcttcac attttttgta
     9601 caaccgacat gcccgtgtag ctcacaaata tgacagtggc gtgaattttg cgcattgacg
     9661 gcagttatga ttcgcggtat tgcttaactg tgattgcata tttagtaatc actgttttct
     9721 tttccaccag aaaccagtat gagggaaacg aggcatgaag agacaaaaac gagatcgcct
     9781 ggaacgggca catcaacgtg gttatcaggc cggcatcgcc ggacgctcaa aagaaatgtg
     9841 tccctatcag acgctgaatc aaaggtcaca atggctggga ggctggcgag aagccatggc
     9901 ggacagggta gtaatggcct gattctgtct ctttaaagag aaacctccgc attgcggagg
     9961 tttcgccttt atatactctg tctgattaaa atcagaatgc agacgtatcc tggaacagac
    10021 ctactttcag gtcgttggcg gtatagatca aacgaccatc aaccagcact tcaccatcag
    10081 ccaggcccat aatcagacga cggttaacta tgcgtttaaa gtgaatacga taggtcactt
    10141 ttttcgccgt cggcagcacc tggcctgtga atttcacttc acctacgccc agcgcgcgac
    10201 ctttaccttc gccgcccagc cagccgaggt agaaccctac caactgccac attgcgtcca
    10261 ggcccaggca tcccggcata accggatcgc cgataaagtg gcatccgaag aaccacagat
    10321 ccggattgat atccagctct gcttcaacat accctttgtc gaagttacca cccgtttcgg
    10381 tcattttgac cacacggtcc atcatcagca tgttcggtgc tggcaattgc gggcctttag
    10441 caccaaacag ttcaccgcga ccagaggcaa gaaggtcttc ttttgtatag gattcgcgtt
    10501 tatctaccat gttctctgta agccttattt tattgaagca cgcaggatag ctaacacgtg
    10561 tacgctgaac aagtccgatc agttcggaat aaaccagttc agccaacgta atggccatgg
    10621 aaaacggtga cgtccttcct gttgcgatgc ttgtgcgata cgttcctgga tggtttgcat
    10681 cagcgtcgtt tggccttcgc catcccacac cagatttaat aataacggca gtgcgtcagt
    10741 cacatcgtct actgcccaga tggtgaattt gccttcttct accgctttca ccagttcact
    10801 atgaagactt aaatggcgaa cgttagcagt ggggataatg acaccttgtt tccccgttaa
    10861 ctcacgttgc tggcaaatag caaagaagcc ttcgattttc tcatttaaac caccgactgg
    10921 ctgggcgcga ccgaactgat cgactgaacc tgtgatagcg atactctgat tcaccggcac
    10981 atcggcgagg gcgcttatca gggcgcagag ttcagccatc gaggcactat cgccatccac
    11041 ttcactgtat gactgctcaa atgtcagcga tgctgagaag gggatctgtt gctcaagctg
    11101 tagttccgac atcaggaacg cttgcatgat catcatccct ttcgcatgga tattgccgcc
    11161 aagctccgct ttgcgttcga tgtcggtgaa ttcaccatcg ccaatatgca caacgcagct
    11221 aatgcgagaa ggttcgccaa aagcgcgtgg atgacccgga aattcaatga ccgaaagggc
    11281 gttaatttgc ccgatgcgtt cgccttcggt ttcaatcagg atttgctcct gaaggatctc
    11341 atcctgcatg cgttcagcga ggaaaccttc gcgccattca cgctgctgta gcattaagtt
    11401 tagctgctcg ccggagaagg tgtcgccatc acacagggag gcgacctctt gacactggcg
    11461 gaggatccac tgcgggctaa gcggaagtgt ttcttgttca ccagtgtagc gtgctgcttc
    11521 gcggataagt accggccagg catccgctcc cggtgctggt aagtgattat gtctggcggt
    11581 aaatgtcacc cagcgacacc actggcttac tgactccgca tcgacaatct gcagagtatc
    11641 ttcaaattcg ctataaatag cctgctctga aagctctggc tccatctcct ggaaatcagc
    11701 caatgattcg cgttcgccta ccagaatgac tttcagcttc aatggcatcg aaggcacaga
    11761 gacggggaga gggcgcgact catcaaacgc aacccagtca aaacgctcgc ggttaacgat
    11821 atttttcagc cgcatccaca gcagaggttg cgccagcagt gtacgtaaag agatgatgag
    11881 aataccgcca tttgcctgat gcaccagacc aggctgcagg gtaatatcgc cattaaactg
    11941 acgcaggcag ccaaagagtt gctctgcttc tacccagtcg gcagcgacaa cttgcgttaa
    12001 agtcgcaaaa ttatcatctg tactcactgc gtgacgtaag cggatggtgt ggccggaaac
    12061 ctcatagtga ccgcccacca gttggcctgc atcgctttgt agcgtacgcg cggcatcagc
    12121 aataagattc agatactcag actcttccgg ggccttcgcc agcataaaag aggaggatgc
    12181 tcgcgtatgc agcaactgct ccagcgcaaa ttgcagtcgc ggttgagtat cactgaataa
    12241 aggatcgttt tcgtcaatca aatgtggctg agcaaatata tcctgatagc tatcggtatc
    12301 aggaaccagg tcacgccatg caagtttcgt aatggtcaaa gttgatgttt tttagtctgt
    12361 tgtcaaagcc gcgattatac cgtaaccggc actgcagcac acgtagaaag caacgacaat
    12421 acgcctggca tgggcgttaa agctcacagg atggagattc ttttcttcac tggcctaaaa
    12481 agctgatatt ctgtaaagag ttacacggta acattgagat cgctatgaaa tatcaacaac
    12541 ttgaaaatct tgaaagcggt tggaaatgga agtatctggt gaagaagcac cgcgaagggg
    12601 agttaatcac ccgttacata gaagccagtg ccgcccagga agccgtcgat gagttgctct
    12661 cgctggaaaa tgaacccgtg ctggtaaatg gttggattga caagcatatg aatccggaac
    12721 tggtcaatcg catgaagcag accattcggg ccagacggaa gcgccatttt aatgcagaac
    12781 accagcatac gcgcaaaaaa tcgatcgatc tggaatttat cgtctggcaa cgtctggctg
    12841 gccttgcgca gcgtcgcggt aaaacgcttt ctgaaacgat tgttcagctg attgaagatg
    12901 cggaaaacaa agagaaatac gcgaataaaa tgtcttcttt gaagcaggat ctgcaggcat
    12961 tgctgggtaa ggaataactg tcaaaagtca gttcaattta ctaaaggcga aaaaaaaacc
    13021 ccgcagctgc ggggtttttc taccagacga gaacttaagc ctgcggctga gttacaacgt
    13081 ctttgatgcc tttaacttcg atctctacgc gacgatccgg agccaggcaa tcgatcagtg
    13141 cagcacgctg tttcacgttg tcacaggtgt tgccagtaac cgggttggat tcgcccatac
    13201 cacgtgcgga gattttgtct gccgggatac ctttagagat caggtaatca acaacggact
    13261 gagcacgacg ctcggacaga gcctggttat aagcgtcaga accgatgcgg tcagtgtaac
    13321 ccagaacaac tacggaaccg tctttcggat ccaggttgct cagctggctg tacagctgat
    13381 ccagagcagc ctgaccttcc ggtttcaggg ttgctttgtt gaaggtgaac agaacgtcag
    13441 acttcagagt gaagtgcttg gtctgtactt ccggtgccgg agctggagcc ggagcaacta
    13501 ctggagctgc ttcgccctga ccgaaacggt aggaaacacc caggctcagc atgccgttgt
    13561 ccggacgagt accgatggtg tgtgcgtcac cgatgttgtt ggtccactgg tattccagac
    13621 gggtagcgat ttcaggagtg atcgcatact caacaccgcc agcgaagacc ggagaaacgc
    13681 cggtatcgtg gtttttacca tcaaagttgg atttagtgtc tgcacgccat accataccac
    13741 ccagacgagt atatacgtcc agatcgtcag tgattgggta acccagttta gcggtcagct
    13801 gaacgccctg agctttatat gcaccgtttt caacgctgcc tttgtatggc atacgaccta
    13861 accagtcata acccatttca aagccaacat acgggttaac ctggtaacca ccaaaagcac
    13921 cagcgcccag ttggttttca tgggtcggac cattgttgtt gatgaaacca gtgtcatggt
    13981 actgggacca gcccagttta gcaccagtgt accaggtgtt atctttcgga gcggcctgcg
    14041 ctacggtagc gaaaccagcc agtgccactg caatcgcgat agctgtcttt ttcatttttt
    14101 gcgcctcgtt atcatccaaa atacgccatg aatatctcca acgagataac acggttaaat
    14161 ccttcaccgg ggatctgctc aatattaact ctaccgatat cttcggctta tgccgagcac
    14221 ccctggcgat gtaaagtcta caacgtagtt gaaaacttac aagtgtgaac tccgtcaggc
    14281 atatgaaaaa aaagtcttgt ataaggtacg tttaatcttt tttgtcagcg acaatttaca
    14341 gaagaaaatc gtggaaaccg cttcagacaa gcctccgcaa ggaaaattag tcacgactga
    14401 aagcattggc tgggcgataa aaaaagttcc aggattaatc ctaaatttac ttaatgatac
    14461 aaattagagt gaatttttag cccggaaagt tgtctcgttg cgtgagagga tgcgcttacc
    14521 ggacgcataa taaaccccat agcgttacct tcatttgccg catcaacaag ttcagcatgc
    14581 tcttcttcag tcaaatcatc tgccaaccaa ccgatcacca cgctgtaatt gcccgtgcgt
    14641 aaagcgcgaa ccattgactc cacggtgtgg caaggggaga gctggctaat ctgcattact
    14701 ttcgttaagg gtagcccaga tgcctgaacc cattcgcgac tcagtttttg ttgcggtgtt
    14761 aaccagagtt gccagcgcga ttgctgaccg agttgctgta acaatggcaa cagtagaagt
    14821 tgcgtcatca tgggctgatc ttcgcgatag acaacttcac tgataagccc ggctgtagtg
    14881 ttttccgtag agacacgcgc aattttactt gctgtggatg agaacgacga agaacgatgt
    14941 gcatagcctg aagtgtacat aatcaatcca gcccctgtga gttactgtat ggatgtacag
    15001 tacatccagt gacgacaaag atcaactcta ttttcggaaa gagcctcgca aattttgtcg
    15061 ttggtgacgg gaaaacataa attaatcttg ccccttaaga ataagttgcc tattttcgta
    15121 gttaacggat ccgttaatgt gaatcattct tttatgttat gattttaaaa ggaattttat
    15181 gaaaagcctc tcctataagc ggatctataa atcacaagaa tacctggcaa cgttgggcac
    15241 aattgaatac cgatcattgt ttggtagtta cagcctaacc gttgacgaca cggtgtttgc
    15301 gatggtttct gatggtgagt tgtatcttcg ggcttgtgag caaagtgcac cgtactgtgt
    15361 aaaacatccg cctgtctggc tgacatataa aaagtgtggc cgatccgtta ccctcaatta
    15421 ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag ctggtgcgtc tgtcgaaata
    15481 ttctctcgat gcagcgctga aagagaaaag cacgcgcaat attcgggaaa gactgaaaga
    15541 tttgcccaat atgtcttttc atctggaagc gattctcggg gaggtgggga ttaaggatgt
    15601 acgggcgtta cgtatacttg gggcaaaaat gtgttggttg cgactgcggc agcaaaacag
    15661 tctggtgaca gaaaagattc tgtttatgct tgaaggggcc attatcggca ttcatgaagc
    15721 tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg gctgactctc ttacgccgaa
    15781 acaggagttt cctgcggaac ttgagtaatc tggcgttgca gacgaccaat ctctggcagt
    15841 aatgcaatca ataacccaac ttgttgtacg accagaggtt ctttgctgtc tgcccgtggt
    15901 tcaagttgct gcatccgctg tttcaggcca gctaatgcct gattgacgcg ttcttcatca
    15961 gcaggttgat gatgtagcgc gtcatcaaca tagcaaacgg cgtcatcaag aaacgccaga
    16021 atttcaggat tagttaactg ctcccggtga gcaccgaggg ctgagatata gctggtaaac
    16081 gtatggttaa ggcacagcaa ccgaaacgcg gcttcgcgaa tttgcggggt aacgttcggt
    16141 tcgctggaca tatttgatac caccgacgcc agttcagcat cacggttgtg tgcatcgcgg
    16201 cgggcaatac gatacgccag acggttatca cgcccctgat ggtattgttc cagtatggca
    16261 tctagatacc ggcagttggc ctctgtggcg cgttcgagca ttcgcggcag attgcgaaac
    16321 ttccagtcag gccagatgta gctcactgcc gcccacgcaa tggcacaacc aatcagcgta
    16381 tcgattacgc gaggtaacgc cacttcaaaa ccttcaccca gcaagttgaa acacagtagc
    16441 accaaaagtg tgatgaacat cgttgcatgg gcgtattgca cgttacggaa ggcaaaaaag
    16501 agcacgccgg taataaccag cagcaccagc tgcccttcca gtgatggcac aaaccacagc
    16561 acaggaatac caattgcgat acctaccagc gtaccaataa tccttaactt cagccggtgg
    16621 cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg tcagcaagat ccaatacccg
    16681 tgatgcattc cggttatctg aatgatggcg tagccgaagc acaacaccag cgacattctt
    16741 accgcatgac ggaagagggc ggattccggc gtgaagtgac ggctaagacg cagccagata
    16801 tcactcaatc cgtgcgggct gtcatcagcg agctcatttt cgtcattatt atggggcagt
    16861 gcctgggcct gttctgattc aattgttgcc agttgggcat caatggcacg taaattgttc
    16921 agtaaaaatc ccagtgtttt gagtaaatca gcaggagcgc cgttatcgcg catccgctcc
    16981 agcgcagcat caatatgcgt aaaagcgcgc tcaaaatgcg gatcatgttg ataaggctga
    17041 cgcagcaaaa tacagcgtga cagttgctgg cacgcctggc cctgcatcga catcagccgc
    17101 tgaaaacgga acagcacgtc gctgtggcga aaatgttcac gcaatgtttg atattgaata
    17161 tgagaagagc tggcacgctc gtgaatatcc tgtgcgacaa agtaataatg cagtgtgcga
    17221 cgcgttcctc gttgaccacg atcgccacgt aagcgggtca gcagcgagag tttcgtctga
    17281 ttcaatgtcg ccatcagctg accgttggcg agagccaaat cgtacagcgg tgcctggctt
    17341 tcatcttcaa tatcaggatc aaacatgcgc gacttgagct caagataacg cgccagttgt
    17401 tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg ggaacagcag atgaccaata
    17461 agtgtcagga cgttgtacca gacggcaccg gccagaagat acatcggctg ctgataccag
    17521 tgctcataca gtgatgttcc caacatagtg taaatggcga tcagcaatgc accgaaggca
    17581 attgttgcat agcgttgacc cagaccgccg agcaaaatga agccgctggt agagagcgtt
    17641 aagccaatcg caaatagcca gggccaggga aacagcaatt ctactgaggc cgaggcgata
    17701 aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc ccgccagtcg gtcatcgaga
    17761 tcggtcagcg ccgctgccac catcccaagc gtcagcggaa tcgtcagttt tacatcaccc
    17821 agccaccacg gaaacgctgt ggttccacaa agcgcaataa aaatacgcgc gtaatacagc
    17881 caggcgctgt tccaggtata gcgtttgagc aaaggactta gcataaaggc catattagtg
    17941 gattattgga aacgacgacg tgcattggct tcgcgcgcag cttgcgctgt ttctaccgat
    18001 accacgcgac gaccgaccgg ccatagtgca atagcggcaa ttttaaagtt cgcaataccg
    18061 acaggaatgc caatgatgga aatacattgt gcgatgcccg ttgcaatgtg catcaggcat
    18121 aaccaccagc caaagaaaat cagccagaaa atattcaata ccgtaccgcc agtatttagc
    18181 agcacatttt tgccagccgg gttcagttca tcgacatgaa tagcttcatt gccataaggc
    18241 accagagaca gtttggtgat ctcccagcag gatcgtgtca gtggtaaggt aaaaatcagc
    18301 acaatactga ccagagtcgc caacagccag cccagagtgg tggcaaatcc gccaagcaca
    18361 aagttcagaa tgttcaaaac ggtacgcata aaacctcgct ttactgtggt tttcagtaat
    18421 gggcggcaat tgtaacgttt ttttgggctg gagcacgttt tctctgacgg ttacactgat
    18481 aagaaataat ttcgtgtgga tctacagagt catggaactg aaagcgacaa cgcttggaaa
    18541 acgtctggca cagcaccctt acgatcgggc ggtgatcctc aatgccggga ttaaagtctc
    18601 cggcgatcgc cacgaatacc ttattccttt caatcaatta ctggcgattc actgtaagcg
    18661 cggtctggta tggggcgaac tggagtttgt actgccggac gaaaaagtgg tgcgtctgca
    18721 cggcaccgaa tggggcgaga cgcagcgttt ttaccatcat cttgatgctc actggcggcg
    18781 gtggagtggc gagatgagcg aaattgcgtc tggcgtttta cgtcagcaac tggatttgat
    18841 tgccacgcgc accggagaaa ataaatggct gacgcgtgag caaacctctg gcgtgcagca
    18901 acaaatccgc caggctttgt cggcgttacc attgccggtt aaccgactgg aagaattcga
    18961 taactgccgt gaggcgtggc gtaaatgtca ggcctggttg aaggacattg aaggcgctcg
    19021 gttgcagcat aatcaggcgt ataccgaagc catgcttacc gagtatgcgg attttttccg
    19081 ccaggtcgag tcttcaccgc tgaatccggc gcaggcccag gcagtcgtta atggcgagca
    19141 ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg tcggtgctgg tggcccgtgc
    19201 tggctggtta ctggcgcgtg gtgaagcgtc tcctgagcaa attttattgc tggcgtttgg
    19261 tcgcaaagcc gctgaagaga tgcacgagcg tattcgagaa cgactacata ccgaagacat
    19321 taccgcacgc acgtttcatg cgctggcgct gcatattatt cagcagggca gcaaaaaagt
    19381 tccgatagtc agcaaactgg aaaatgatac cgctgcccgt catgaacttt ttattgctga
    19441 gtggcgcaag caatgcagcg aaaagaaagc gcaggcgaag ggctggcggc aatggctgac
    19501 ggaagaaatg cagtggtcag tgccagaagg taatttctgg gatgatgaaa aattacagcg
    19561 tcgtcttgcc tctcgcctcg atcgctgggt aagtctgatg cggatgcacg gtggtgcaca
    19621 ggcagaaatg attgccagtg cacccgaaga gattcgcgat ctgttcagta aacgtatcaa
    19681 gttgatggcc ccgttattaa aagcctggaa aggtgcgctg aaggcagaaa acgctgtcga
    19741 tttttcgggc cttattcacc aggcgattgt gattctggag aaaggtcgct ttatcagccc
    19801 gtggaagcat attctggttg atgaatttca ggatatctcg ccgcagcgtg cagcgttgtt
    19861 agcggcatta cgcaagcaaa atagtcaaac gacgttgttc gctgttggtg atgactggca
    19921 ggcgatttac cgattcagcg gtgcgcaaat gtcgcttacc acggctttcc atgaaaactt
    19981 tggtgaaggt gatcgctgtg atttagacac gacctaccgt tttaacagtc gtatcggtga
    20041 ggtggcaaac aggtttattc agcagaaccc aggccagctt aaaaagccgc taaacagctt
    20101 aaccaatgga gacaaaaaag ccgtcacgtt attggatgag ggtcaacttg acgctttgct
    20161 ggataaactc tctggttatg ccaaaccgga agagcgcatt ctgatcctgg cgcgttacca
    20221 tcacatgagg cctgccagcc tggaaaaagc agcaacacgc tggccgaagt tgcaaatcga
    20281 ctttatgacc attcatgcca gcaaagggca acaggcggat tacgtcatca tcgttggctt
    20341 gcaggagggg agtgatggtt ttccggctgc ggcgcgggag tcgattatgg aagaggcgct
    20401 actgccaccg gttgaggatt tcccggacgc tgaagaacgg cggttaatgt acgtggcgct
    20461 gacccgggca cgccatcggg tatgggcact gtttaacaaa gagaatccct ctccctttgt
    20521 ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa ccgtaagaaa caggtggcgt
    20581 ttgccacctg tgcaatatta cttcagacgg tccgcgagat aacgctgata atcggggatc
    20641 agaatatcga ccgcgtcgtt gaaatgcggc gactggataa tgaagtctgc cgttgccacg
    20701 ttggtggcaa ccggaatgtt ccataccgtc gccagacgca gcaaggcttt cacgtcagga
    20761 tcgtgcggaa cggcatttag tgggtcccag aagaaaatca atacatcaat tttcccttct
    20821 gagatcaatg cgccaacctg ctggtcaccc cccattgggc cactcaacat cgcgttgacg
    20881 ttcatgccgg ttgcacggga aatcaagtta ccggtagtgc ctgttgcata cagtacgtgt
    20941 tgttccagta acggttgatg tcgttccacc cagctcatca gcatttgttt gcagtgatcg
    21001 tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc gtgtcgtcag ttccataatg
    21061 tacatccgta gttaactttc ctacagatta ctgtaagcac ttatcgctgc aagagaaaga
    21121 ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggagga tggttgtttt tccgcccatt
    21181 gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa ccaatacttc agccgttgtt
    21241 ctgtgagcac ctgagactgc ggcggaatag catccagctc ggaaacaccc gaaagcagcg
    21301 tactgtcatc tgccatcatc gtggcaaatt gcggtagtga agcgcgtttc gcggatttgt
    21361 tatagcgttc aacctcgacc tcataatcaa tcgtttttgc agttgaggta atggcgagaa
    21421 tatccagctt taccggaatc ggcgtcgcat cgccatccaa caattcaagg cgcggcgcgg
    21481 catcaaaatg gttagcttcc cgctcattct ccaggcgagg caggcgaaaa ttgacctggt
    21541 tgatgagctg ggtattaaaa ctaaccacca gtggagggga gatatagagc cgttcttcac
    21601 tattggaaag atgaatcgtc ttctcaacgc gaaacactaa ctgatgcggt ccgttatcca
    21661 gttcaatgct gtccgcacca cgcagcagag agctggaaac cttcttaccg tcgagtacca
    21721 gaagatcgac atcggtagaa agccgcagcg tggtggcaaa aacggatacc ggcaaacata
    21781 atgcaatcaa ggtggtcacg atgccggttt tcatagatag ctcctgttaa aagaccgccc
    21841 gaataataat gtgtcaaaat ttttctttaa tcatacttac taacatatag acatatttcc
    21901 cgcttttgct ctcattcatt cgtattagct gcatggttgg catgtcggct ttgtcgtaca
    21961 ctttgttaaa cagtctggag gaaataatga aagaaaccga tattgcaggc attttaacgt
    22021 ccactcacac cattgcgctg gtgggggcaa gcgacaaacc cgatcgtccc agctatcgcg
    22081 tgatgaaata tctactcgac caggggtatc acgtcattcc tgtttcgcca aaagttgccg
    22141 gcaaaacact gctgggccag caggggtatg gcacgctggc agacgttccg gaaaaagtcg
    22201 atatggtcga tgttttccgg aattcagaag cggcgtgggg cgtagcgcag gaagcgatcg
    22261 ccatcggcgc gaaaaccctc tggatgcaac tgggggtgat taatgagcaa gcggcagtac
    22321 tggctcggga tgccggatta aatgtagtga tggatcgctg cccggctatt gagatccctc
    22381 gcctgggcct ggccaaataa aaaatccccg gaaggcaaaa accttccggg gatttgttca
    22441 gggattagtt acgcagacgc ggggcctgga gttgtttgcg gatggtctgc gccagctcat
    22501 ccatggatgg ctgttcagga tgttcatcct gcaattcgct acttagctgg gcttcagcca
    22561 ggtaggtatg aaccggtagg ccgttatcgt cctccatcac cacgtgatac cacggagcgg
    22621 cgcgaagctc gtcattcacc gccagctcat caggcgacgg ttcagagagc gaataaaccg
    22681 ggtcgatatc cacgaccact ccgagataac ctaacaggga atggcggacc tgctggccga
    22741 taccgaattt gctggcaatc atagtcacct cccgggaaat ctttaccttt gatataaggg
    22801 taaaattcca catttcaagt tacatgacgc gacaggcaaa ccctttcaga tatagccctt
    22861 ccggataggt agcgatcacc ggatgatcgg ctgcctgacg gaactgctct ataaattgta
    22921 catcacgacc ggcatcaatt gcggcatccg cgatgatttt ctgaaataaa tcgctggtca
    22981 tcagaccgga acaggagaaa gtcaggagaa taccgccttc attcagcaac tgaatcgcca
    23041 gcatgttgat gtctttataa ccacgacacg cgcccatcaa ctggctttta ttctcaacaa
    23101 acttcggcgg gtccatcacg ataacgtcaa atttttcacc gcgatcgcga taggtacgca
    23161 gcaatttaaa gacatcatca cggacaaact cagccttgct cagatccagt ttgttcagct
    23221 caacgttctg ccgtgcaata tccagggctt cctgggaggt atcaacgctg acaacctggc
    23281 tgcaaccgcc catcagtgcc gatacggcga aaccaccggt ataggagaaa cagttcagta
    23341 cgcgtttatt ttcaacgtag cggcgggtag ccaggcggct atcacgctga tccaggtagt
    23401 agcccgtttt gtgtccgtgt tgaatatcca ccagcagctt catgccgtgt tcttcaatcg
    23461 gcagcagggc aggcggtagc tctccggtaa cggggccctg ggtcagttcc atcccttctt
    23521 ttttacgtac cgcaacgtcg ctgcgatcgt aaatcgcaca ttccgggtac agcgtttgca
    23581 gggcactaat taatgctgcg cgctgatatt ctgcgccagc actcagcaat tgcagcacca
    23641 gaaaattacc gaaacgatcg atagtaatac ccggcaggcc atcagattca ccggcgatta
    23701 aacgatagct gtcgaggcca tctttttgcg ccagccagtc acgccatttt tgtgcttgtt
    23761 gcaaacggcg ggaaaaaaaa gcaatgtcga tagactcaga cgggtcaaac gtccagacgc
    23821 gcgcccggat ttgcgaagct ggcgaataag cgccgcgtgc taaccatttt ccctgatgat
    23881 caacaatatc gatggtttca ccgaggctgg ctttaccttc catgcgggca acggccccgg
    23941 aaaagaccca cggatggcga cgaagtaatg atttttcgcg ccctttggct aacactaaac
    24001 gtacactcat aatttacttt tctgtcgatg caaagaaatg ggtgtcattg tccggagttt
    24061 tccatggatt tgcaacgcgt ccattaagga taaggggagc agcaatgtcg aaagtctgca
    24121 taattgcctg ggtttacggg cgagttcagg gcgttggatt tcgctatacc acgcagtacg
    24181 aagcgaaaaa actgggacta accgggtacg ccaaaaatct tgacgatggc agcgtggaag
    24241 tggttgcctg cggtgaagaa gggcaggtag aaaaattaat gcagtggtta aagagtggcg
    24301 gcccgcgttc tgcgcgtgta gaacgggtgc tcagcgagcc gcatcatccc tcggggaaat
    24361 taaccgattt tcgtatccgt taaatacatt ttaccggttt tggcaggcca gcaattttgg
    24421 tggcttgctt agccggacct ttcggaaaca gtcggtataa atagcggctg ttaccttttt
    24481 cttcgccaaa tttattcgcc atcgctttta ccagcatacg aatcgccgga gaagtattga
    24541 attccagata gaaatcacgc acaaaacgca ccacttccca gtgttctggc gacagcgtaa
    24601 tcccttcgtt ctctgcaatc accaccgcca gcggctcact ccactggctg ctttctttga
    24661 gatagccttc ggtatccgtt tctatctctt taccttcgaa gatcagcata attattacta
    24721 ccttaatcag actggcggca gtgtaacaaa aaacaaagcc ccgcataaag cgaggctatg
    24781 aaagtgttag cgggtaagat taatcgcggc tggcgaagcc cagaatgctc agcaggctga
    24841 cgaagatgtt gtacagcgaa acatacaggc taaccgtggc acgaatatag ttcgtttcac
    24901 cgccatgaat gatgttgctg gtttcaaaca aaatagcgcc agaggagatc agaatgaaga
    24961 ccgcgctgat cgccagatgc agagcaggca gttgcaggaa gatattagca accataccaa
    25021 tcagcaccac cacaataccc gccatcagca taccgccgag gaacgacata tctttgcggg
    25081 tggtcagcac atatgcagag cagcagaaga acactaacgc cgttccgccc agtgccatag
    25141 cgattacgtc acccattccg gcagacagat aggtattcag aattggtccg aggatataac
    25201 ccagaaaacc ggtaaaggcg aatgcggaga taattccggt cggcttattc gccgttttat
    25261 aggtcaggaa catcaaacca tacataccca ccagcgtcag aatcagaccc ggagatggca
    25321 gcatcagcac cgtactggca gttgcggtaa tcgccgaaaa ggccagcgtc aggctcagca
    25381 gaaaataggt attacgcagc accttatggg tgctaagcag tgatgtacgg tcatgtgaag
    25441 aactaacaat acgatccatt gagtcactct ctatgacaga tgtaattaat taagcagcat
    25501 aatgataatg cgtaagggca cccagaagtt ttacccatct ttacgcattt gatctggaac
    25561 aggtttaaca gcggattatc aggtcattaa gcaatgatat aacgctctga gaatttcgac
    25621 aggtaaaaga aaaaggggtt agcatttagc taacccctta tcttatttgg cggaagcgca
    25681 gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccgaag aaaaacaaat
    25741 aaaaatcagt gtaatacgtg aagctgctga aaatttaatc aaatttaaca ccaagaaaaa
    25801 tcaaggtgtt aggaagttgc tcatagctag tataccaagt atttttgcca gaaaattttc
    25861 tgtattgcct acttgctaac cacgagcggc agatccagtg ttggtgtgat ttttgtttta
    25921 cgatcgtaaa tcaacacctg attttctgtt ttatgcccgc tgaaaatttg tttgtcgcga
    25981 ctgctgcctt cgtaatcaga gatccccttg gcttttatat cgtgaaaatt gcacccgaac
    26041 ggaacgccaa ctttttgctc ggctgagcgt ttagcctgat tccaccagtt gttcagcgtt
    26101 ttagctatga ctttcccgcc tttggttgta ttgatcacat attcgcatgt acaggaagat
    26161 acatttcggg ctaactggat cgctgtacgt aatcgcggtg accattcctt gatttgtttg
    26221 gtgccggttt tgttttgctc aatgtagatc cctttatcca taatatcctg ccatttcaac
    26281 tcaagcacat caccgagcct tgccgcacag agataggaaa tctccattgc aatgcgtaac
    26341 tgtggaattg cttccgcata tatcgctgca tactgttcat cggtgatgta aacagtgcgg
    26401 gctttaagag agaattttct gactcctttg catgggttat tcttcacgta tccacgctca
    26461 tatccccacc cgtatactcg actcaggctt gccagttcat gatttgcctg ggtcttgctc
    26521 tcaagccctc gtttatccat gaaaattctt acctgctcag ttttgacatt atcagcaagc
    26581 acttttccga ataccatcag caacgccttc tgatgttgtc gataatcttt ttgggttcgg
    26641 ggggccagtt ctgtaaatgc aggggagtcc ataaacatgt gccataattt agctacggtc
    26701 attatgttgt ggagttttgc tttttccagt tcataatttt gccagacttt agctacgctg
    26761 gttttccgca ctcttcctag ccctatagtt cttgtacttc cttctggttt ccacacgtaa
    26821 ctgtaaccat tcgatctaac ccgcggtggc agtacattat cttttttatt ttttcttggt
    26881 cttcccattg ttcagcgcct caaaatcggg ttcagcagaa accagttcag atgtttttgg
    26941 catcgttgtc agtccgtgtg gaatatccct gcgtagaact attggttcgt ttttaggacc
    27001 gattacaaat gggatgccgt gcagtcttaa ctggtgttgc tgttttgtgt atcgctcgta
    27061 tttcgtgatc tctttaatct ctgctggaga tagagttaat tcgtacatgt ggtcacgtgc
    27121 ctttacagca tgaccgccgc cactatagct ggtgggcggc gatatgggtt gaacattaaa
    27181 aatcagcctg actcgggatc agtttttgcc agatagctga aacgtatttt gcctgataac
    27241 gggcgtcatc aagtgcatta tggcgctcac cttcgaatgg aatagccgtt ctggcatcga
    27301 agtctatggc tttccccagc tcaacgattg tgcgtacatc gcgatcgttg tagtaacgcc
    27361 acgggcaggg gatcccctgc cgttcgtatg aacggcgcaa aatcgtgttg tcgaagttgg
    27421 ctccatttcc ccagacctga acaaaaaatt caccggagtt ttcgtcgata aattcccgca
    27481 gttgtaacag tgcatcatct aacgggattt catcggtcat aatggcagat tgcgcttcgc
    27541 gtgattgctt aagccaccat ttaatggtgt cccgatcaat gactccgcca gcagtttcca
    27601 gatcgatagt cttactaaat tccggtccca tatctccggt ttgcggatcg aaaaatattg
    27661 cacctattga gatgatcggg gcattaggat tttttcccat ggtttcaagg tcgatcatta
    27721 gatggtcaca cgtcctgctg gtggatgtga tttcgtgatg accgttcacc gcaattaagg
    27781 gatctgccgc ttcgccagtt tcactatcgc tggtgtgatg ctgatcgtta ccagcattct
    27841 cattgttcgg atgttcagcg ccttctattt cctccggatc atcttcctga acttcagcct
    27901 gatttttttc atcgaatgtt tcctggtatg ttgcgtcgcc catcaccgcg ccacagtcag
    27961 gacagttgcc gccaccgctc tgaccgcagg cggtgcagac tttttcctct tcctgttgcg
    28021 ctactggttc ggattgtttc gtttctggct cgtcttgtaa cgcattttgg ctgttttgtt
    28081 ctgctttctg gtcgttctgt tccgtttcta gctggttctg gttcacagaa tcgcaggttt
    28141 caatcccctt aacccatttc gaatcattcg ggtcgctaat cccttcaaca aactcaccac
    28201 gagaagcggc aagcaatttg tcggcatctg gtcgactgat gtctgctgcc tccataattt
    28261 tatttacttc ttcagcggta acttttacct gtgacttgtc atccagcggc tgcgggtcct
    28321 gatgatgttc aactgtatcc gctgccattg tttcagccgt tgcctgttca tctgccattg
    28381 cgccagatgg ttgcgggttt tcttcatcat ttttttcttc ttctgttttg cactcagcag
    28441 ccagtccgtc gttaatttct tccaggatat ctttttccgg tgtatgccgg gcagccatga
    28501 gcgtttcagc tgtgggattc tcgtgatcag tttctgtcag gttggcattg atatacccct
    28561 gaaggcgtcc cgggtagtga taaaactcag ggtgggcgct ccggataagt gcaaaaatag
    28621 ctgcgcggga atagtccagg ataccagggg ttgcgcgaag tgctgcggac cattccctga
    28681 acggactttc ttttttcagg acgatttctt ttgcgcgacg ataaacgctc acgggtattt
    28741 cataaatatt aaaatccatc ggaagcgtgg ctgctgcagc ctccacatcc agagtatcga
    28801 gggtgtgtac taagtcagga ttacgatcgg ttttatttcc gccaccggca tttgcaccgg
    28861 aagccgtgcg ggtgatgcga gaaacacgat ttcctttcat ccactctttt gtcagcagac
    28921 ctcgatcggt atagtcagcg tccaggtatg cttcgaaaaa agcagtcatt aaccccagac
    28981 tggaattaac gggattaggg aaaactttgt ctgtgtcgcg taccagttta tgaaggtcgc
    29041 gaatctccag cgggtcgagc agctttgtgt tgtgggaaat agccagggca gtaacagccg
    29101 gcagttcttc agctcgtgcg atatgtaatg cctgaagttc ttcccgtgaa acgtgcgtta
    29161 ccggtttttc gctgccatgt tgcgcaagcc agcgaatggg tagttcctga ccggaaattg
    29221 gcaggagcat attatcctca atctcagcca tgtcttcgcc attgacgttg gtattgtcag
    29281 tgctggctgg tttttcctga actgaggggg aaggcgctat aaatatcatt gtgatgccat
    29341 cttccccgcc tttttcatag cggttgcaga attcagtatc aaacacgcct tccggtggaa
    29401 ggtcattcac aacggggaaa tggacgcgga cgggtttttt gaaatcatca gcgtcgtagc
    29461 ctgcctcgtc cattgcaaca acgcaccgcg agattgctac agataatttc ttttctgtac
    29521 tccagtaaaa acccccttta atcccaaagc gttttctgac cttgtcattt tttgcttcgc
    29581 aatatagcgc aaattcttct ttatcagtgc tcattgataa acctcataac tattttaagg
    29641 ttgtacgaac ccctgccatt gctggcatgc ttaatcaacg ggtatggcgt taatatggct
    29701 ggtggattat ccagccggta tttcgttatt caggtacagc gatacttttt ttaacgggag
    29761 gcattcgcca gaaatttttt gctcgtctct tgcccggagg catgattctt tactggcata
    29821 aattccggta atcacattct gtggctcacc cgttataaga aaaacagtca ttaccagtgc
    29881 aaatgcagaa gtcactgctg ccccccgaaa ataccaagtt taagaagggc aattctggag
    29941 agtatggaat tatcattgag aagataaggc tcatattttc tcatcttaat ggcatcttca
    30001 gtaaactccc ggttactgag cagaacacca atatcaaaac acccttcaga cgtattaacg
    30061 tttggtaata acgtttccat tatcgtgtcc tcaacaatga attttgtgat gtggtgcctg
    30121 gtgcctccag gtgacgttaa ccagttaaca attaacgccg gaatacagaa ggatgcccgt
    30181 tacgccccgt aaaagaccac tttacggatt taactgttcc gcgtgcgcat agccgcattc
    30241 accgcatcac aaaattcact ttaaaaagga tggcagagca gtcatggagt cgaactgatg
    30301 ccaccaaaga ctacacatgg atattgtggc ggggctgtca cttaagcgta tggtcagcct
    30361 gacaacccgg tgtcctcaag gggaaggaat aaccccgcca tacttaccgc cgcgccattt
    30421 cgcggattgc cacaaccgga agcgcacggt cgaattaaat ttaacgacaa agtacagaga
    30481 gacgagcttc tccgtgcgct ttcgtgttat gccctgactt ttcagggtaa attaaccagt
    30541 ggcaacctgt ttttactggt gctaatcagt tagtgtttct ggctaaccag cgacgcgcgc
    30601 cagcttcggt tttaaacttt ttacttttgg tatacgtcat cgcggtaaac gtgccgtcct
    30661 ggttagggaa cacaccgcat accagagatt cgttgttgcc aagattgagc gtatccatgt
    30721 tgacctcatt tccccttaac gccggggtag cggaacaaaa aacctgctgc atagttaaac
    30781 gtaatccccg ccgtcatgtt catacgcctc aggctggcta cttaacccct gaccactgcc
    30841 gggtaactcg aagtattgcc ctgcgttctg tgggtcgggg t
//
LOCUS       DAZKHK010000056        30807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKHK010000056 DAZKHK010000000
VERSION     DAZKHK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.056"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(480..743)
                     /gene="stxB2b"
                     /locus_tag="ACHOS5_002491"
     CDS             complement(480..743)
                     /gene="stxB2b"
                     /locus_tag="ACHOS5_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP9777298.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            complement(756..1715)
                     /gene="stxA2b"
                     /locus_tag="ACHOS5_002492"
     CDS             complement(756..1715)
                     /gene="stxA2b"
                     /locus_tag="ACHOS5_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP9777299.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            complement(1807..1883)
                     /locus_tag="ACHOS5_002493"
     tRNA            complement(1807..1883)
                     /locus_tag="ACHOS5_002493"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1847..1849),aa:Arg,seq:tct)
     gene            complement(1897..1973)
                     /locus_tag="ACHOS5_002494"
     tRNA            complement(1897..1973)
                     /locus_tag="ACHOS5_002494"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1937..1939),aa:Arg,seq:tcg)
     gene            complement(1981..2056)
                     /locus_tag="ACHOS5_002495"
     tRNA            complement(1981..2056)
                     /locus_tag="ACHOS5_002495"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2021..2023),aa:Ile,seq:cat)
     gene            complement(2097..2222)
                     /locus_tag="ACHOS5_002496"
                     /pseudo
     CDS             complement(2097..2222)
                     /locus_tag="ACHOS5_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            complement(2498..2932)
                     /locus_tag="ACHOS5_002497"
     CDS             complement(2498..2932)
                     /locus_tag="ACHOS5_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9777300.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(2925..3119)
                     /locus_tag="ACHOS5_002498"
     CDS             complement(2925..3119)
                     /locus_tag="ACHOS5_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP9777301.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(3116..3679)
                     /locus_tag="ACHOS5_002499"
     CDS             complement(3116..3679)
                     /locus_tag="ACHOS5_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9777302.1"
                     /translation="MQQRKPKKCKVCGTPFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRNLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHNRHRW
                     TVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            complement(3687..4136)
                     /locus_tag="ACHOS5_002500"
     CDS             complement(3687..4136)
                     /locus_tag="ACHOS5_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFP9777303.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNSPELNNRLLSFF"
     gene            complement(4136..5107)
                     /locus_tag="ACHOS5_002501"
     CDS             complement(4136..5107)
                     /locus_tag="ACHOS5_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP9777304.1"
                     /translation="MQTKKAAVGHWGRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMELLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRKLVMDKFSRLSCLRGTTGEAYLQGRGILQLPTESVRFCDRQIARGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSRNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            complement(5097..6617)
                     /locus_tag="ACHOS5_002502"
     CDS             complement(5097..6617)
                     /locus_tag="ACHOS5_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP9777305.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDRDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVRNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYSREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAD"
     gene            complement(6611..6874)
                     /locus_tag="ACHOS5_002503"
     CDS             complement(6611..6874)
                     /locus_tag="ACHOS5_002503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777306.1"
                     /translation="MHVARDVTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSV
                     MKLRELARRGTEGFVLLRMPKKLTSYSDAGIEIIPANGKGASC"
     gene            complement(7186..7464)
                     /locus_tag="ACHOS5_002504"
     CDS             complement(7186..7464)
                     /locus_tag="ACHOS5_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777307.1"
                     /translation="MPDQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFIPIHDGG"
     gene            complement(7511..7750)
                     /locus_tag="ACHOS5_002505"
     CDS             complement(7511..7750)
                     /locus_tag="ACHOS5_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9777308.1"
                     /translation="MTLDEYLRQSSIRQADFAAIVGVSQGFVSGVITGRYMPQGRKAI
                     EWAKATDWNVTPHELNPHDYPNMEDGIPLHVRQTA"
     gene            7856..8575
                     /locus_tag="ACHOS5_002506"
     CDS             7856..8575
                     /locus_tag="ACHOS5_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9777309.1"
                     /translation="MTKKITITDKDIQNAERLRKIWDEKRKQLSLSQEKAADILGFKT
                     QGAVSQLLNAKIALNTENTLKFAALLQVPAEEINPDLSDLLRSIRTHTPGNRRNLFEK
                     YYEYPLLSCVQAGAFSMDDFSYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGK
                     PSFPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIEC
                     NENCQFVGKVIKSQWNDETFD"
     gene            8697..8867
                     /locus_tag="ACHOS5_002507"
     CDS             8697..8867
                     /locus_tag="ACHOS5_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777310.1"
                     /translation="MNKDKSLTQQVEEILSKQKVSEQGKMIQSLKNMGLINKPVFTLA
                     YGPDITTCQMHQ"
     gene            complement(8982..9326)
                     /locus_tag="ACHOS5_002508"
     CDS             complement(8982..9326)
                     /locus_tag="ACHOS5_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9777311.1"
                     /translation="MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELE
                     QEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNK
                     ALEKEVNHHSGA"
     gene            complement(9323..9571)
                     /locus_tag="ACHOS5_002509"
     CDS             complement(9323..9571)
                     /locus_tag="ACHOS5_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9777312.1"
                     /translation="MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRF
                     FNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGVL"
     gene            10418..10969
                     /locus_tag="ACHOS5_002510"
     CDS             10418..10969
                     /locus_tag="ACHOS5_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777313.1"
                     /translation="MMRKILRTTLIATILFSPIVQAEWYTVTDDDLFSDGHTALMVGK
                     KTSNPEEIDNNMIALNCTPRSLTFSLVEKDIEEETSAPIYSFPIDMAIKIDKNSIIRL
                     NATLSRRNSMLTQAITERTDTEMKKIKIILRQLRDAKHRVRVGIRRQDITREDAMTYS
                     FSFNVLGSTGAVNKFITACGIKL"
     gene            11236..11874
                     /locus_tag="ACHOS5_002511"
     CDS             11236..11874
                     /locus_tag="ACHOS5_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFP9777314.1"
                     /translation="MNSQLIPVFNGTIDNETTLFCNARDLHAFLEVGKDFSTWIRIRI
                     SEYEFTENQDFILLPKTGEQRKGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRQSLLPAPMNINYPLSWFSDNHPYATMNYVDRKVISLDASVLFDMPSPTMR
                     ILNELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRFGFNVNL"
     gene            11945..12157
                     /locus_tag="ACHOS5_002512"
     CDS             11945..12157
                     /locus_tag="ACHOS5_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP9777315.1"
                     /translation="MLKPHYGTALVSREEVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            12169..12450
                     /locus_tag="ACHOS5_002513"
     CDS             12169..12450
                     /locus_tag="ACHOS5_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9777316.1"
                     /translation="MLNQEININVKSVITPAKTIMGEVFMDDKIIAYFVVLPDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            12471..12752
                     /gene="gamL"
                     /locus_tag="ACHOS5_002514"
     CDS             12471..12752
                     /gene="gamL"
                     /locus_tag="ACHOS5_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFP9777317.1"
                     /translation="MNAYLTCDRIEERRWANQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            12769..13719
                     /locus_tag="ACHOS5_002515"
     CDS             12769..13719
                     /locus_tag="ACHOS5_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFP9777318.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARGNLSSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKTWDDLLPLCSKTFRRTIASHEY
                     LSQEEAVKTLDFVKKKAARNKATAEAKIHATTENNSEAVS"
     gene            13716..14405
                     /locus_tag="ACHOS5_002516"
     CDS             13716..14405
                     /locus_tag="ACHOS5_002516"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9777319.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVSE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGLDAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGVNN"
     gene            14405..14992
                     /locus_tag="ACHOS5_002517"
     CDS             14405..14992
                     /locus_tag="ACHOS5_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFP9777320.1"
                     /translation="MDEVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADGYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRRKNVNPNHTDDPGYWQYGSDSF"
     gene            15067..15414
                     /locus_tag="ACHOS5_002518"
     CDS             15067..15414
                     /locus_tag="ACHOS5_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777321.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            15478..16299
                     /locus_tag="ACHOS5_002519"
     CDS             15478..16299
                     /locus_tag="ACHOS5_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP9777322.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADEHSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKRQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAIRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLGNRE
                     FTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEEEDILTFIGTFSV"
     gene            16376..16819
                     /locus_tag="ACHOS5_002520"
     CDS             16376..16819
                     /locus_tag="ACHOS5_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777323.1"
                     /translation="MQFKDLPEDIQKIAADTLKAHLSVLDLTKEPKANLENISRNVRN
                     VFVGLYAYDNEKHEEHIQNGCINKCQQNIKLPVADKTEQKTDVLVFWNTVEAIARSTM
                     LEFNNINHSGIDTHQSSHREGFSILLTLLQEQGEYPQPTSNDTEQ"
     gene            16927..17805
                     /locus_tag="ACHOS5_002521"
     CDS             16927..17805
                     /locus_tag="ACHOS5_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFP9777324.1"
                     /translation="MSTHHVVSFSGGRTSAYLVHLMEQRRKAGENVHYILMDTGCEHP
                     LTYRFIREVVKFWDIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGQGNYITWLGIRADEPRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGLACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDGIARMYADSDYSNLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            17802..18005
                     /locus_tag="ACHOS5_002522"
     CDS             17802..18005
                     /locus_tag="ACHOS5_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777325.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
     gene            17998..18237
                     /locus_tag="ACHOS5_002523"
     CDS             17998..18237
                     /locus_tag="ACHOS5_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777326.1"
                     /translation="MFKQNEKSIAQIAEYIPRTCRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNCHTRESFFSCIAAQINKGGNQ"
     gene            18234..18869
                     /locus_tag="ACHOS5_002524"
     CDS             18234..18869
                     /locus_tag="ACHOS5_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9777327.1"
                     /translation="MSKIDYQVLRDVSERAITAMTHLSILPGDDDLLSEKKLKELGID
                     IDAIHAFKIMAGPETVLSLLDERDALNERMAELEANLAELAEDQQKAIESIKQADAAV
                     KLAHEKFSALAAENAVMKKFCKDAAFDADYEAELGMERGGFSDALNDIETPATDAFLA
                     EVRAQAFNDLCSVFVKDATVVGLDDGDIVTVKEAKDALLHCAEQLRKGGNQ"
     gene            18866..19060
                     /locus_tag="ACHOS5_002525"
                     /pseudo
     CDS             18866..19060
                     /locus_tag="ACHOS5_002525"
                     /inference="COORDINATES: protein motif:HMM:NF025303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            19382..19567
                     /locus_tag="ACHOS5_002526"
                     /pseudo
     CDS             19382..19567
                     /locus_tag="ACHOS5_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            19572..19760
                     /locus_tag="ACHOS5_002527"
     CDS             19572..19760
                     /locus_tag="ACHOS5_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777328.1"
                     /translation="MNRTIIVTTVISVPANAPHQDIDDFVESKFGQDKDMKPDNLCYG
                     DAAEIISHHWEYGSHYMN"
     gene            19848..20066
                     /locus_tag="ACHOS5_002528"
     CDS             19848..20066
                     /locus_tag="ACHOS5_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9777329.1"
                     /translation="MVTLFRKKYPRKSRATEFLFLILFIVLMIPISPLLLLWFIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKEN"
     gene            20068..20355
                     /locus_tag="ACHOS5_002529"
     CDS             20068..20355
                     /locus_tag="ACHOS5_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777330.1"
                     /translation="MATLQELIDLTPEQEKAWNRLVKAVKDFRAAGGKFYSVLDTLSA
                     YNGEHVASIDNDKGYHTASVYMPSIDAPGLTSWADDWHGITLKDGVEVDKD"
     gene            20359..20490
                     /locus_tag="ACHOS5_002530"
                     /pseudo
     CDS             20359..20490
                     /locus_tag="ACHOS5_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20503..20982
                     /locus_tag="ACHOS5_002531"
     CDS             20503..20982
                     /locus_tag="ACHOS5_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9777331.1"
                     /translation="MGIELVKYGCNTFICPDNSMEPGNVPLYIGLPRIDPASQTAKLS
                     FQEWLSEQKEKIDVDCGCVSIETLTHWMKSAYEAGNSPVTPDSWISCSDRMPEKGQNV
                     LISVNFDSSLVEPLICSAHYTGSTFRRGDATIKPGNGIEQATHWMPLPEPPQEVNRG"
     gene            20975..21259
                     /locus_tag="ACHOS5_002532"
     CDS             20975..21259
                     /locus_tag="ACHOS5_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9777332.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYEGYEIKTITHTHFGDKPVKVFAIKVNIGSD"
     gene            21308..22048
                     /locus_tag="ACHOS5_002533"
     CDS             21308..22048
                     /locus_tag="ACHOS5_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777333.1"
                     /translation="MINNIANQSSFPAATIDSQMLLKMVNEARKLCSEKPVRNNDFIA
                     RVKDELEGETYEIFVGQKNGAEIEVITMTFKQALRVAARESKAVRRSLVDKLESMQQQ
                     LQQKTTTKKSPDGLEEFRKARALKMTVDTMKDLFDFLPNLAPEAKQVVAASLVNPVVG
                     ANVIPLPMINEHYYSASEVGAKLKISANKVGRIANTYMLKTEQYGKWFIDKSPYSDKQ
                     VESFRYNNRGVRKIEEILDAENKAEFGT"
     gene            complement(22091..22396)
                     /locus_tag="ACHOS5_002534"
     CDS             complement(22091..22396)
                     /locus_tag="ACHOS5_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9777334.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NTTISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(22486..22758)
                     /locus_tag="ACHOS5_002535"
     CDS             complement(22486..22758)
                     /locus_tag="ACHOS5_002535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777335.1"
                     /translation="MSLAAPIRDPIMNIIARFFYLIIFCMATSGCTTTTKINRGDEKE
                     QYIIACGAATPWGLCYDKANALCKNGYKDILKEQGFNRKELTIECK"
     gene            complement(22812..23069)
                     /locus_tag="ACHOS5_002536"
     CDS             complement(22812..23069)
                     /locus_tag="ACHOS5_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9777336.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAMEAWLIHSAAPPLDAIRENLGKGHSISQVRSEIRARK"
     gene            23394..24071
                     /locus_tag="ACHOS5_002537"
     CDS             23394..24071
                     /locus_tag="ACHOS5_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP9777337.1"
                     /translation="MTTQVSVETLSTITYKQIPVITTELLAHLYGTEAIRIRQNHHEN
                     KGRFIEEKHFFKLEGETLREFKHRVAFNYSVKIARNVRSLILWTERGAARHAKMLETD
                     RAWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLD
                     VSVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSR
                     KGFSLSL"
     gene            24127..24558
                     /locus_tag="ACHOS5_002538"
     CDS             24127..24558
                     /locus_tag="ACHOS5_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP9777338.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            24555..25181
                     /locus_tag="ACHOS5_002539"
     CDS             24555..25181
                     /locus_tag="ACHOS5_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFP9777339.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            25141..25353
                     /locus_tag="ACHOS5_002540"
     CDS             25141..25353
                     /locus_tag="ACHOS5_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9777340.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPTV"
     gene            25389..25769
                     /locus_tag="ACHOS5_002541"
     CDS             25389..25769
                     /locus_tag="ACHOS5_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9777341.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIASVQPVQPVQPVQPVQHNIIGDLLRKSRKEARRAGRKQK
                     RWEGACKALQELNKYRDLINELSE"
     gene            25913..26146
                     /locus_tag="ACHOS5_002542"
     CDS             25913..26146
                     /locus_tag="ACHOS5_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777342.1"
                     /translation="MKITFESYGLTASVVISSSIFESRKHQHIVDAVKLKAPEVTVTT
                     HGLFRIRTTLTTTHLSACRVYDMALKEYNQCSS"
     gene            26134..26385
                     /locus_tag="ACHOS5_002543"
     CDS             26134..26385
                     /locus_tag="ACHOS5_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9777343.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            26446..26628
                     /locus_tag="ACHOS5_002544"
     CDS             26446..26628
                     /locus_tag="ACHOS5_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9777344.1"
                     /translation="MEINLMTEKEVSELLQKRRTALYNLRKKHGFPDPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(26612..27781)
                     /locus_tag="ACHOS5_002545"
     CDS             complement(26612..27781)
                     /locus_tag="ACHOS5_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9777345.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPIFTQ
                     VEPYIERLLNIGFDVLFPGQEIDKPIDRSSANLYMKKLRAKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            27977..28051
                     /locus_tag="ACHOS5_002546"
     tRNA            27977..28051
                     /locus_tag="ACHOS5_002546"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28010..28012,aa:Arg,seq:cct)
     gene            28259..28411
                     /locus_tag="ACHOS5_002547"
     CDS             28259..28411
                     /locus_tag="ACHOS5_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777346.1"
                     /translation="MLSLMNSQARDTRDRSLNYDNALEISEPCIRSTFERLYQNMNEA
                     FGLGDY"
     gene            complement(28491..29738)
                     /locus_tag="ACHOS5_002548"
     CDS             complement(28491..29738)
                     /locus_tag="ACHOS5_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP9777347.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDQEHQCVAADVGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(29807..30724)
                     /locus_tag="ACHOS5_002549"
     CDS             complement(29807..30724)
                     /locus_tag="ACHOS5_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP9777348.1"
                     /translation="MPAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSAVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDREICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRQI
                     IDLAQRCGALAVTAKGAMTALPCRQELEW"
BASE COUNT         8767 a         7464 c         6917 g         7659 t
ORIGIN      
        1 tgagtcagat aaggggcggg ctgaagtccg gaagttacag gacaatggca gaaggaagac
       61 tacagcccga aatacgaaaa aggccgcgca gttgcgcagc cttatgaatc ctggttaaaa
      121 tccattcgat tataaaaatg tatatctcat gctattgccc gaacccactc gggctttttt
      181 ttgcccacaa gaaagcccct ccggagaggg gctggtgtat gcgctatgta ccgttcatgc
      241 atggtgccgg gtgcctcccg gtgagttcag tatcagcacc tgaacccgca cagaaaggat
      301 agaggtaaaa cagaaacacc tgtgacgctg atatgcccct ccgctcaggg ggattcacca
      361 tgcaggattt ttttaacaaa ttctcagtcg ggcagacaac cttcaactga ctgaattgtg
      421 acattgatta cactgtaact acattgctgc acaccacgaa accagcaatg cttctgatat
      481 taattaaact gcacttcagc aaatcctgaa cctgacgcac aggtatttga tttgattgtt
      541 accgtcattc ctgttaactg tgcgctttgc agtagcggtt gcagattcca gcggttagtc
      601 cagtactctt tcccggccac ttttactgtg aatgtatcat tctcattata cttagagaac
      661 tcaattttac cttttgcaca atcagctgcc attgcattaa cagaaacaaa agcaaataaa
      721 gccgctacaa atatcttctt catatttaac tcctgttatt ctccggatgt atttaaagag
      781 tgagccctgc gattcagaaa agcagctgcg gtattacttt cccataacgt attgtttatc
      841 cttataactg gcctgtcgcc agttatctga cattctggtt gtatctcttc attcacggcg
      901 cgaacggaac gcgccccctg atgatggcaa ttcagtataa ccgccactgt gcccagtatc
      961 gctgatatat tattaaagga tattcgcccc actctgacac caccctctcc ccgaaactcc
     1021 ggaagcacat tgctgattct cccccagttc agtgtgaggt ccacttcttc cggtgtcatc
     1081 gtataaacag gagcagtttc agacagtgcc agacgaaatt ctctctgtat ttgcctgaac
     1141 cgtaaggctt ctgctgtgac agtgacaaaa cgcagaactg ctctggatgc atctctggtc
     1201 atggcatttc cactaaactc cattaacgcc agatatgatg aaaccagtga gtgacgactg
     1261 atttgcattc cggaacgttc cagcgctgcg acacgctgca gagtggtata actgctgtcc
     1321 gttgtcatgg aaacagttgt cacaccgggc actgatatat gtgcaaaatc tgaaaacctg
     1381 tagaaagtat ttgttgccgt attaacgaat ccggccacat ataaattatt ttgctcaata
     1441 atcagacgaa gatggtcaaa acgcgcctga tagacatcaa gccctcgtat atccacagca
     1501 aaataactgc ccggtggggt gtggttaata acagacaccg atgtggtccc ctgagatata
     1561 tgttcaagtg gggtcgatat ttctgtccgt atactattta acgaagagac ataactttgt
     1621 tgggtcgaaa agtctatcgt aaattcccgg gaataggata ccgaagaaaa gcccagtaac
     1681 aggcacagta cccattttaa caatatacac ttcatataca ggtattcctt ttggctgaag
     1741 taatcagcac cagacccggc gcagatataa aaaaggcccg caaaagcgag ccagaaaaaa
     1801 taagtatggc gcgttgtact ggattcgaac cagtgaccga ttgcttagaa ggcaattgct
     1861 ctgtccggct gagctaacaa cgcagtgtac agataatgga ccgccatcga ggactcgaac
     1921 cccgcgcaac cagcttcgaa ggctggcgct ctgtcccgat gagctaatgg cggtatgtga
     1981 tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct cgctctcacc
     2041 actgagctaa agggccgggc gcaggataat aacggtacgt aactaatcct gcaatatcat
     2101 ccgttctgac tggctaaatc ctgaacttcc ctgaccgtct gctcaaaacg ttcagtctcc
     2161 agctcaacgc cagttgcacg acgccccagc gccattgctg ctttgacgct acggacataa
     2221 aaaagccagc cactggggga ggctggcaaa ctcgtagagc aaaatgctgt tacgcaaact
     2281 tcgttacagg gttatcctgc aatacttaaa atatacaata tttagaaaac taatagtgcc
     2341 atatgcgatt tttaagattt tgttattaat tgcggtcgca ccttcctttc tgtgtacttt
     2401 ccgtataact aacaggattc cggatacaaa aaaacccgcg catcggcggg ttaagcagcg
     2461 tagcaatgta accactctta tcatgatatg cagattttta cgatcgtaaa ctattttttc
     2521 gctgataaaa tacagaggtt ctccctcccg gcaattcacg ctcaacatac cgatccatct
     2581 caagcctcac tcccagcatc atcagcatgc cttcaacaat cccctccgct ttgtgaaggc
     2641 gtttacctat acaggtgtca gagcacccat gtttccgtgc cagcgccatg aacgtctccc
     2701 ccaacacgta gtaatcaacc agcaagtcat gcagatcgcg attgttccgg taaaggcggg
     2761 ctatgcaccc gcatatcacc atcgcatcat cgtcacagca ctgtggacgt gattttactt
     2821 tttcggggat cagtccctta aatccggcag caatgggcga ccatgtaaca tcctcatagt
     2881 tatttgccgc ccatgcaccc cagcgctcaa gaacctgccg gatatcacgc atcagtatct
     2941 ttaccccatc cgcggtgaac cataagaaca ccgttgacga tagcgtgtct tttcccttct
     3001 ttatcgccaa tgtattttct gactgtggca cgattgcagt tcagtattcg ggctacctct
     3061 gtctgatttc catatgcctc aacgagcatg tcaggaatgg tttttactgt gaacgtcatg
     3121 cggcctcact tctgctgttt cgcaggtctt taagtttctg ctgatacttc gccttgatcg
     3181 ccctgcattc ttcgacagtc cagcgatggc ggttatggtt cgattcgatt tcgtctactg
     3241 cttcctgccc gatgcggtta atcagttcga cgcgatacgg aacgagattt ccgcttttgt
     3301 actggttaca caccacgcat tgcttgtgga tattgcgttc atcaaatctg agttggggag
     3361 ccgcagctgt tgtacggtaa tgacctgcat cccactgagc agaatcgaat gtcccgcatg
     3421 aaacgcacgg caaattacgg tctcgttccc tgatataagc attcacggcc tgctgtgcct
     3481 gcttaatcca gtaactgcgg ggctgtaagt cccttcttcg cgctttaagt ttgtctgcct
     3541 gttcttttgc agaggctatg gccttttctt ttctgaccag ttcaagtgcg cactgaccgc
     3601 aacaaacttt ctgatacgag cgaaacggca caaacggggt tccgcacact ttgcattttt
     3661 taggtttacg ttgctgcatc atcacctcag aaaaacgaca gcaggcggtt attcagttca
     3721 gggctgtttg agcgcccaaa cacatgcttt aacgcggcat taatcattgc gttgtagcaa
     3781 cgttcaaact catcctgatc catattcgcg taactcaggc tttttgcccg atatctcacc
     3841 tcgcctctta tggttgtgac cacatcatag aacccggcaa gaattgtcag atttttacga
     3901 aactcatcaa actgcgtcgc ttcatcagag aattcgtatc cagcatgttc agcgcaccag
     3961 tactgaaagc agaaattaag aaaagcgaac atcttccggt gaaaagatgg gttacgggta
     4021 agttttgctt ccagtgtata cagctcaccg tttttaaatt ttgccagtcg gggtaaatcg
     4081 cgctcatacg ccggtacaaa tacgccatta gccgccttga tcatctctat ttccatcagg
     4141 cagcctccgc tttgaatttg cgcgtcagca catgttcacg ggcttcacat ccctgaataa
     4201 gcatgtcgtt aaaatccggc aagtcaggcc agcgaatact gaccttctca acgtcattac
     4261 gactcatgag attcttatgc ccacatttaa aggcagccgc cagacctgcg ccgtgcgcat
     4321 cattgtcagc aaatataatc aggtgattaa cgcctggtgg cgcaataaac ttctccataa
     4381 aaccggagtt cattgttgac cacacattgc agcggaatat ttgacggcat gaaagcgccg
     4441 tttcgatacc ctcggctatc cccagagtgg acgacaccgg atacaggcgt attgccaccg
     4501 atttggcatg ctgcaaacca ggcaactcct gtagcgcggt cattttttta gccgcctcta
     4561 cattcgcctt gcgatcacca tccagcaacg tacgatgcag atagcaaaga gaacctttgt
     4621 catctgttgc aatggcgtaa attgcctgat attcgcgccc tctggcgatc tgacggtcac
     4681 agaaacgcac ggattcggtc ggtaactgga gtattcccct cccctgcagg tacgcctcac
     4741 ctgttgtgcc ccgcagacat gaaagtctgg agaacttatc catgaccagc ttccgctttc
     4801 tgcttatctc tgttacaggc tggctgactt tctccctctt ccaggtattt ccgatcagcc
     4861 gatcgatttc atcacacaac gtcacccagg gcttacccgt ggcaagttgc aacaactcca
     4921 tcccgtcccc gtgaccacag acgcaaatcc acgaaccgga gccatcttta tcatcacagc
     4981 gaaatttacc tctggcacca catatcgggc agggtccgtt gtaatgtttc acaccagtaa
     5041 caggaggcat gccgtaatat tcaaaaattc gcccccaatg cccgaccgct gccttcttag
     5101 tctgcatgct ggcctcctgt catggctttc ttgcgccctt tggcataggc aatggtcttt
     5161 gacttgatga agttgtacac ttcaactgag gtttgcagcg gcgcattact gaatcctttg
     5221 ggccaaaccc cgaacttctc cttgtaggta tgagcacacc atccgtcact caggggttta
     5281 cccgtcgcgt tgcggtaatt ctgatacccc ttgatctcac tccaccaacg ctgcttttcc
     5341 tcgcggctgt attcgcgttt ccctttgttg acgcgtgaaa gcttacgatc gcgatctgtc
     5401 gccacgtcat caccaccaag aggacgaaat ccacatttgg gacacatatg cacgccagca
     5461 ggcttcatga agtggcattt ggggcattcc ctgggaagtt tctcagcctt aacctcactc
     5521 ccgccagcag acgctttcat tccgtcattt tttcccggaa gttcgtcata ctcgatatcc
     5581 tcggggaagc ccagccgatg aaccgtaccg gaatggtcga aaatcaatgc gcgtttttta
     5641 ccagacgcag tacgtaacgc tctcccgatg cactgcaacc agcggatttc tgatttggtc
     5701 ggacgggcgt agattaggca gcgaacatcg ctgtcaaatc ccgcaaccag tacgccaacg
     5761 ttgacaatta ttttggtcgc accctcttca aaacggcgaa tgatgtcctg ccgttcatca
     5821 tggggggtat ctgccgtcat cacctctgcg ccaatgccag actgtaaaaa ctcacgggta
     5881 acaaagttcg catgagccac gttcacgcag aagcaaatcg tcggtaaatc ctcgccattc
     5941 tcaagccagt tgcgaacgat atcgccaacc agatccgaac tccccatgat ggatgcgagc
     6001 tgctcctcgt tgtaatcccg tccaaatacg gtgttactgg tttttacgcc agccagatca
     6061 ggcattgagg gggcaaaaaa ctcataatcg ctcagatcac cacgctgaat aagttcacga
     6121 atcgttgtag gcttgatcag gcattcgtag tacttcccca tccaggctgc gaacggggtg
     6181 ccggaaagtc caagaaccct gatgtctctg tcccggataa cctcaagtaa cgcacgacgc
     6241 ttcatgtgtg cttcatcgat gatcagcaga tcgatgttgt caggaaactc acgacggatc
     6301 agcgtgtcgg cactggcaat ctgaatcagt cgtgacgggt catagttagg gtgatcacgc
     6361 catacaaaac tgatttcttc ccacggcaaa ccgtattcgg tgaaacgttc cgctgtctgg
     6421 ttcagcagga tggtgtacgg acagacaaac atcactcgca taccacgttc aaccattccg
     6481 gcggtgacga atgctgcaag accagttttg ccagagccag tgggtgcata catcaggtac
     6541 gtgcgatgct gtttccactg gtgccgcagc tggtttaaac cgcgctcctg agcaaagttt
     6601 ggggtgattt tcagcatgac gcccccttgc cattcgccgg gatgatctcg atcccagcat
     6661 cgctataact ggtcagtttc tttggcattc gaagcaacac aaagccttca gtgccacgac
     6721 gtgccaattc acgcaatttc atgactgagc ggggtcgcgg agagctacga tcgatctcaa
     6781 tagccacact cccaccagtt tttgctgtcg cgagaatatc aacacggaaa tttctgccat
     6841 cgattcttac tgtgacatct cttgccacat gcattccctg tgactcaagg atcgttacaa
     6901 catcagccag aaagctgtaa cggtcctctg ttctcagtgg tacagtgctc agtgtacgga
     6961 tcagtgtcga ttttattgct gctgacatag tttatcactc aatagttagc ccgataatta
     7021 tttcaccaga aactatatcg cgcttataca gtgatctacc taacctatgt acctgccttc
     7081 tggcctaggc caagggatac aggccttacc aacgactcca cccccttacc ccctcctcgc
     7141 ttcctttcag gaaattaatt gcacatgaaa ttatttctag cgcaattaac cgccatcatg
     7201 tatcgggata aacttccgta ccgctactgg cgaaagcatc cagccacggc tggctctcgc
     7261 gtacttctgg acatacaccc gtaaccgggt gttggcgctc cgtcttgccc tgttgccctt
     7321 cctgaacgat acaggctcct tgtcccactc ttcctggtac actctcgcgt actctgctgc
     7381 aatttttacc ctggtggtcg ggtctagctg taacaattgc tcctgtatcc agtcccgatc
     7441 agcatcacag tactgatcgg gcataaccgt tttgatttgc ctgttcactc acacctccga
     7501 tggagggtta tcaggctgtt tgccttacat gaagcggaat accatcttcc atgtttgggt
     7561 agtcatgcgg gtttagttca tggggagtta cattccagtc tgtagctttt gcccattcaa
     7621 tggctttccg cccctgaggc atgtatctcc ctgtaataac cccactgaca aatccctgag
     7681 aaactccaac aatcgccgca aaatcggctt gtctgataga gctttgtctc aagtactcgt
     7741 caagagtcat agagatcctc ccatttcaat ttttcgatat tagcttacct gatttaaatg
     7801 tcaataggct agctatttga taaatatttg tgttgctaat aaaatcggag gcactatgac
     7861 gaagaaaatt acgattactg acaaagacat ccagaatgct gaacgactgc gcaaaatctg
     7921 ggatgaaaaa cggaaacagc tatccctcag ccaggaaaaa gccgcagata tcctcggctt
     7981 caaaacgcag ggggctgtta gtcagttatt gaacgcaaaa attgcgttaa atactgaaaa
     8041 tacccttaag tttgcggctt tattacaggt tcctgcggaa gaaattaatc cagatctgag
     8101 tgatttgtta cgcagtattc gtacgcacac accgggaaac aggcgtaacc tgttcgagaa
     8161 atattacgaa tatccattac tctcctgcgt gcaggctggc gcattctcaa tggatgattt
     8221 ttcgtacacc gcaaaggatg cgattaagtg gatctccacc accacaaaag ccagtgacag
     8281 gtcgttctgg ctggaggtca aggggcattc aatgaccgcg ccacagggag gtaaacccag
     8341 cttccctgaa ggtatgctga tacttgttga ccctgaacgg gaaatcgagg atggcgattt
     8401 ttgcgtggcc cgaatgaacg gcgatgaatt caccttcaaa cggttcattc gtgagagcgg
     8461 taaagcgtac ctggagccac tcaacccacg attcgacatg attgagtgta acgaaaactg
     8521 ccagtttgtc ggaaaggtca taaagtcgca gtggaatgat gagacttttg attgaactca
     8581 caggatgata tcaataacct tgcagggagg ctattttatt tcctctttca gcgaaaaatc
     8641 tccgagaacc ctcgcccaat gcgggcgggg agcagtcgca atcaagtgag ccataaatga
     8701 acaaagacaa atccttgaca caacaagtgg aagaaatact tagcaaacaa aaagttagcg
     8761 agcaaggaaa aatgattcaa tcattaaaga acatgggctt gatcaacaag cctgttttta
     8821 ctttggctta tggaccagat attacaacct gccaaatgca ccaataacaa aacccggcct
     8881 tggtgccggg ttttgtttgc ctcctgctcg ccccaccatt taccagtgcg ccgtaaattc
     8941 cccattcttc ggcggtgagg atgtcaaaga cgaagggaac attaagcccc tgagtgatgg
     9001 ttgacttctt tttccagcgc cttattaaca aaggcgttca gtgataaatc ttcttccatc
     9061 gccatttcag cgacccgacg atgcagttct ggatcaagtc tgacgttaaa cacaccttta
     9121 aacggggtat caggctcctt tccatcctcc acacaagact gtaaatacag ctcaaccgat
     9181 gtcttaaact cctgttccag ttcagctaat gtagaagcct cataagttac caggtctcga
     9241 ataaacgcca gttttccgta caggatatta ttttcaaaat ctggttctac tgtacctaaa
     9301 tatcctttat attttagatg cttcataata ccccagcctc tttcagattc tgtttaatcg
     9361 ctttcagcgt tccaccttta atataacttt ctggatgtgg gcgatgcatt aatatggtgt
     9421 ggttgatttc agcattgaaa aaccgcactc ttgagccctg catttccttt ttgacatatc
     9481 ccagagagga aaacaaaacg accagctcat cccattcaaa cgttttttta ctgtttaaaa
     9541 acttttccag tagcttatct gctttcccca tacccacata atctcatctt cgctacattg
     9601 caactaatta tagttacagg cagtgttttt gtcaaaagcc accaagcccg ctgggttctc
     9661 tttgactgtt cgctgctttt cctggtgtta tccgggggct tttccctcac gccaaatctc
     9721 acacagagta accggcctca gcgtcggttt ttctttgcgc atcctccata actcaccacc
     9781 tgcattatcc tcgcttatat ttttttacat aaataaatta atctaaaaat caatcacata
     9841 gattaaaatt aaatcaaatt attagcctta ctattgacta tgataagttg agcttattat
     9901 attttaccca agccagaaac atcgcgccct tacacagggc tacataaatc agtcgtacgg
     9961 cgcgacttaa cccgccgcaa aatgctcttt aacaatctgg agctttacag cgtcaatgac
    10021 ctgtttagac ccctacacgt aaacgtgctg tatcatcggg tgcgatccgg tcgatgagag
    10081 agtatccccg cgcgagagcg agaacggcgt gagaacgggc aacactggca ggaagttggc
    10141 gctgaccaat acagggaatg ttttggggtg ctgagagtgc caccaaagcg ttgcgtgaaa
    10201 tagcgatgcc gtcccaaccc tgtagcaaaa gtaatgattg gataccataa gaccaatcac
    10261 aatcattcta cgattaccta tgccgcacaa agttattttt tcaacacaat gttcaagttt
    10321 tcaactgact tctttatact accgcgtagg tagtgctatc aaatagacta tgtaagcact
    10381 tcatgaagga tgtcggtcga actgaacggg aaacttaatg atgcgaaaaa tactcaggac
    10441 aactcttatc gctacaattt tgttttcacc aatagtacag gcggagtggt atacagtaac
    10501 agacgacgat ttgttctctg atggtcacac ggcgctgatg gtaggaaaaa aaacatcaaa
    10561 tcctgaagag attgataata acatgattgc tcttaattgt acgccaagat ccctaacatt
    10621 ctcattagtc gaaaaggaca tagaagaaga aacatcggcc ccaatttatt cattcccaat
    10681 agatatggca ataaaaattg acaaaaatag tataataagg ctcaacgcaa cactttcaag
    10741 aagaaactca atgctaacac aagctatcac tgaacgtact gatactgaaa tgaagaaaat
    10801 taaaattata ctcaggcaac tcagagatgc taaacataga gtgagggtcg ggattcgacg
    10861 ccaagacatc acacgagaag atgccatgac atattctttc agctttaatg tattaggctc
    10921 cactggtgct gttaataaat ttatcactgc atgcggaata aagctgtaaa catgtttctg
    10981 ccaaagcaac cttgacaaat ccacatcgac ggggatatat tccacctcat ggtgctgaac
    11041 acaccttgca aagcggagac cgcacccgtc agtcatgcgg cttttttatg tccatttttc
    11101 agatatggtc gggtagcgcg tataccgaaa aacagccgaa aggttaagga tacgggccga
    11161 ctttgcacgg tgttcaagta cctggccgcc ctgctgaaca cagggctatc tgaacaaatg
    11221 caaaggacat aaaatatgaa ctctcaactc atccccgtat tcaacggcac tatcgacaac
    11281 gaaactaccc tgttctgtaa tgcccgtgat ttacacgcgt ttctcgaagt cggaaaagac
    11341 ttttccacgt ggatacgaat ccgcatctcc gagtatgaat ttacagaaaa ccaagatttt
    11401 attttgctcc ccaaaacggg ggagcaaaga aaaggtagag gcggtcacaa ccgcaaggac
    11461 taccacctca ctctcgatac agccaaagag cttgcgatgg ttgaacgtaa cgaaaaaggc
    11521 cgccagatac gccgatactt catcgagtgc gaaaagaaac ttcgccagag ccttttacct
    11581 gcaccaatga acatcaacta ccctctctcg tggttctcag ataaccatcc ctacgccacg
    11641 atgaactatg ttgatcgcaa agttatcagc cttgatgctt ccgtgctctt cgatatgcca
    11701 agcccaacta tgcgcatcct caatgagcta cacagcaagg gctataacgt tgacgccgct
    11761 gtcgccgaat ttaacgcctt caaacatctg acggaagaaa tgcgccgtaa attgctggat
    11821 atttcccgca catcggaaaa gtcttcccgc tttggtttca acgttaatct ttaattaacc
    11881 ccatccccga tcacatatcg gggattaaat taaatatctg gattaattaa ccggaggatt
    11941 tgtcatgctc aaacctcact acggaaccgc attagtttct cgcgaggaag ttaaacccgg
    12001 cactgcaatt ctttacaacg ggcgttatta catggcgtca gctaacgtta ataatgcact
    12061 ttacgcacat tcactgattg aaaaaattcg cattatctca gatgcaatag aagtttacct
    12121 gaacaataaa ggccaaccgt taatctcacc agcctgaaag gaaatatcat gctcaatcaa
    12181 gaaattaata tcaatgtaaa aagcgtcata acgccagcga aaacgataat gggagaagtt
    12241 tttatggatg ataaaatcat cgcctatttt gtcgtcctgc ctgatgaggc tatttctgtt
    12301 attgatacgg aaggcaatgt tatgtttatc gcggaacatc cagaagacat cgcattacag
    12361 gccgctgcat atttcttcgc taaggagcaa gaggaagaat gtaactgccc tgtctgtcag
    12421 ctttcccgac aaattaattt aatgcattaa ccggaatcag gagttccgcc atgaacgcat
    12481 acctcacctg cgaccgcata gaggagcgtc gctgggctaa tcagcaaatt caggatgaaa
    12541 aggataaatg gattgatgat cgggcgcagg agcttatcag catgttcccc gataaaccat
    12601 tgcttatgag cagccttttt ttacccaaag aagcccaact ggcactcact ggcgaaaaag
    12661 ctgaagaggc gtacaacgat tatatctcag cgatcgccta tgctcgggcg gaagaagaat
    12721 gggagagaaa attctccccc tgtcctttct gattttcagg accaaaaaat gttcgatatc
    12781 gttgaatttg ttaagcagca ggagcgcttt ttctgcgagg cattaactga accaacgctg
    12841 acatgggcga aggaaagtca gtttgcaatt cagcaattcc agaaaaatgc ctttctggct
    12901 gacacagcac ggggaaatct gtccagcgca cagaacgcta tcatcaatgt tgccgctata
    12961 ggcataaccc tgaacccggc cagcaagctg gcgtatctgg tcccacgaaa aaaggctgta
    13021 tgcctggata tcagttatat ggggcttctg catctggcac aggtcacagg agccattcag
    13081 tgggggcaat gcaaacttgt ttacgagaag gacatttacg agtccaacgg tattgactgc
    13141 gcccccacgc acaaatacaa cccattcgta gacaggggcg cacgcattgg cggttattgt
    13201 gtcgtaaaaa catccgaagg cgactatctg accgaagaga tgagcaacag ggaaatcgag
    13261 gtcatcaggg cgtgcagcaa agccggaaat aacggaggaa gtagcccgtg ggatagcttc
    13321 cccgacgaaa tggccagaaa agccattgtt aagcgcgcca gcaaatactg gccccgtcgc
    13381 gatcgcctgg atacagctat cgactacctg aacactcagg ccggtgaagg tatcatcctg
    13441 aatgctgatc atatccctga gcgtgacgtc actcccgcat cagatgagat tatcaatgag
    13501 atcactcagg caatcaccga aattaacaag acatgggatg acctgcttcc cttatgttcc
    13561 aaaacattcc gtcgcacgat tgcatcacat gaatatctca gtcaggaaga agctgtcaaa
    13621 acgcttgatt ttgtcaaaaa gaaagctgcc agaaacaagg ccacggcgga agcgaaaatt
    13681 cacgccacca cggaaaataa cagcgaggcc gtgtcatgac accagaaatt attctccagc
    13741 gaactggcgt ggatattacc agcctcgacc agggcgatga tggatggcac aagctgagac
    13801 tcggtgttat cactgcttca gaagttcaca atgtgatagc aaagccacgt tccggcagca
    13861 aatggcctga tacaaaaatg tcatacttcc acaccctgct ggctgaagtt tgtactggcg
    13921 tggcaccgga agttaacgct aagtcgctcg catggggaaa gcaatacgaa gatgatgccc
    13981 gtgccctctt cgaatttatc gcggatgtta ccgtctcgga aacgccaata atttttcgtg
    14041 acgaaagcat gcgcaccgcc tgctctcccg acggtttatg cagcgacggt aacggtcttg
    14101 agcttaaatg cccttttact tcccgcgact tcatgaaatt ccggcttggc ggccttgacg
    14161 ctatcaagcc tgcttacatg gcccaggtgc aattcagcat gtgggttaca gacaaggacg
    14221 cctggtactt cgccaactac gacccgcgca tgaagcgtga aggcctgcat tatgtcgtgg
    14281 tcgaacggga tgaaaagtac atggcgagtt ttgatgagat ggtgccggag tttatcgaca
    14341 aaatggacga agcactggca gaaattggtt ttgtatttgg agaacaatgg ggggttaata
    14401 actaatggat gaagtgattt ttacttataa cgaagaatca gcactgaccg ccggacaagg
    14461 tggttttatt accgaaacgg gtgcgcatat cattaacatc accgaagcag aactcaagca
    14521 atcagaaaaa ggtgcccgat tcattgagtt ttctggagaa tccgacgacg gacggaaaat
    14581 ccagtatctc agtgtttgtg ttcagaagaa tgacggcact gaaaataaat ttggggcgag
    14641 cattattcac gccatgatgg gatgcacagg aattgggcag ttaacgcagc atatggtttc
    14701 cgtcagtaaa tatgttgcac ctgagtttca cggaaagaaa atcggactcg tcctccagaa
    14761 agtattaacc acaaacagaa agactggcgc agacggttac cagatggaaa tccgcattcc
    14821 attcattgca gaaacagggc agacactgaa agaaaaagct gaaggaaaga aaccagaaac
    14881 ggtagcaaat atggtttcca ctctcaaaga caaagacaat cgcagaaaaa acgtaaaccc
    14941 gaatcacacg gacgacccag gttactggca gtacggcagc gatagttttt aattcacaaa
    15001 caaaaccagg ctttaaatgc agtgaacaac tgaagcctta acacacctct acacggagat
    15061 attaatatga accagcatca aactgatgtt aatgttttca ttaacgacct cgacggcggg
    15121 gtatttgtta acaaacttgg tgcggtatta agtgaagttg cctttggcgt aaacagcaca
    15181 aacaaaaaag gaaaggtatg tgttgaattc gaattatctt cacttgatga aaatcgcgta
    15241 tcagtttccc ataaactaaa attcacacgc ccgacaatgc gtggtagtaa atcagaagaa
    15301 gacacaacta acaccccgat gtttgtaaat aaaggtggtg agcttacttt gttccagaaa
    15361 gaccagggac agcttttcga taaacagggt caacatgacg ctgttttacg ctgaataatc
    15421 cccgccttaa aacgctcgcg cattatccct aaatccataa attaaaggta aatatacatg
    15481 tctcagttag atagcaatgc cattaaagaa attgtaaaac tcaccactac tgctttttct
    15541 ggtgaaaatt tgccgcttac ggaatgccct gttgcgttat tgccggacaa tgtaaatatc
    15601 gaaagtctgg aacgattcat gacagaacgt tttcgtttcc gtggagtcat gaccacaacc
    15661 agtattgatg actttgttga atacagcaaa gggtatgccg atgagcattc ccgctgcttc
    15721 attaacgcgg aaacgatgaa agcagtcact gtgttcaata tcggcactct ggagcaacct
    15781 ggacacgctg acaacaaggc actgctggaa ctgaaagcca catcaccata tcgcgcatta
    15841 cgtgacgtgg acggcaaaag acaactccag aaatctctcg ctgaatggct ggaagactgg
    15901 gccgacttcc tcacagccta tgacagcgac ggtaatgtgc tggatatcaa acaggctatc
    15961 tctgctatcc gtcgtcttac catcgacgca aaacgcagtg ctgaatatga agagcaaaac
    16021 ttcagcggta gccgctcagt catggagtcc gtggaggcta aaagcaaaga aatcatgcct
    16081 gccactttcc gctttgaatg catcccatac gagggcctgg gcaacaggga attcacgctg
    16141 cgattaagca ttctgacaag cgaacaacct gttctggtgc tacgtatcgt gcgtgttgaa
    16201 gctgcggaag aagaaattgc caaagagttc cgtgacctgc tgaaagagcg tttcgaagaa
    16261 gaagatatct taaccttcat cggcacgttc tcagtgtgat gaaatctgtg acagggacgt
    16321 cacgacatat cgctcatatt tcacgagaaa atacacagtt cctggagggt atactatgca
    16381 atttaaagat ttaccagagg atattcagaa aatagctgcg gatacgctta aagcccattt
    16441 atcggtgctt gatttaacaa aggaaccaaa ggcgaatctg gaaaatatat cccgtaacgt
    16501 gcgaaatgtt tttgtcgggc tgtatgctta tgacaatgaa aagcacgagg aacatattca
    16561 aaatggttgc attaataaat gtcaacaaaa tatcaaactt cctgttgctg ataaaacaga
    16621 gcagaaaaca gatgtattag tattctggaa taccgttgag gctatcgcca ggagcacgat
    16681 gttggagttt aacaacatca atcattccgg gatcgacact catcaatcat cacatcgtga
    16741 ggggttttcc attcttctaa ctctgttaca agagcaaggc gaatatcctc agccgacatc
    16801 aaacgacact gagcaatagt ccattctcgt acgctgcaac gtgaggtcgt accccggaca
    16861 cgggtaaaca tccggcaatt ccagcttacc acctatttaa ccccctcccc gtaacgagac
    16921 gaagaaatga gcactcatca tgttgtgagt ttttcaggcg gtcggacatc ggcgtacctc
    16981 gtccacctta tggagcaacg cagaaaagct ggtgaaaacg ttcattacat actcatggat
    17041 acgggatgtg aacacccgct gacgtaccgg tttattcggg aggttgtgaa gttctgggat
    17101 attccgctaa ccgtgttgca ggtcgatata aatccagagc ttgggcagcc aaatggttat
    17161 acggaatggg aaccaaagga tattcagacg cgaatgccgg tgcttaaacc gtttatggac
    17221 atggtaaaaa aatatggcac gccatacatc ggcggcgcgt tctgcactga cagattaaaa
    17281 ctcaccccct tcacaaaata ctgcgatgac catttcggac aagggaacta catcacgtgg
    17341 ctgggtattc gtgcagacga accccgtagg ctgaaaccga aaccgggcgt ccggtatctt
    17401 gccgaactgt cagattttga taagtcggat gttatccggt ggtggcgaaa acaacctttt
    17461 gatttgcaaa tcccggagca cctcgggaac tgtgttttct gcatcaaaaa gtcaacgcaa
    17521 aagctggggc ttgcatgtaa agacgaacct ggtctgatgc gggtttttaa tgaactggtt
    17581 acaggcaaac acgtcaggga tggtcatcgc agaacaggta aagacgttat gtaccgtggt
    17641 cacctgacgc ttgacgggat tgccaggatg tatgccgaca gcgactacag caatttgtat
    17701 caagcgatgg tgctggccaa gcggtttgat actggttcgt gttccgaatc atgtgaaatc
    17761 tggggtgacc aattggagtt gaaattcgaa gaggtagtgg catgacaatc gtaaaaaccc
    17821 ataccggcat tgtgatcacc aaagacggcc cgcaggtaaa aaaacttcac cagacagagc
    17881 ggatgtgggt cgtcggcaaa aacgagtttt accacaaaga aaccggacgc cgccactttg
    17941 cagaaaatac gcgccgcaga ctgctgatcg acacaatcaa gcctgtcgag gtgaagcatg
    18001 tttaaacaga acgaaaaatc tatcgctcaa attgctgagt atatcccgcg tacgtgccgg
    18061 ggtatgcagt tgcaggaagc caaagcgcgc ctggagaaaa aaattgcgct ctatatcgat
    18121 gacggctgtg atgctgccgt tcttaacgcg gctttcgcac cagctcttaa ctgtcatacg
    18181 cgggaatctt ttttttcgtg catcgcagcg cagatcaaca aaggaggcaa ccagtgagca
    18241 aaattgacta tcaggtattg cgtgacgtgt cggaacgcgc aattacagcg atgacacatc
    18301 tgtcaatatt accaggtgat gatgatttat taagcgaaaa gaagctcaag gagcttggta
    18361 ttgatattga tgcgattcac gcctttaaaa ttatggccgg gccagaaacc gtgctgtcac
    18421 tactggatga acgagatgca ttaaacgaac gcatggccga actggaggct aatttagcgg
    18481 agctggccga agaccaacag aaagcgattg agtcaattaa gcaggctgat gcggctgtta
    18541 agttggcaca cgagaagttt tcggcgctgg cggcggagaa tgcggtgatg aagaagttct
    18601 gcaaagacgc tgcattcgat gccgattacg aagcagagct aggtatggag cgtggtggat
    18661 tcagtgatgc gcttaacgat atcgaaaccc cagcaaccga cgctttcctg gctgaagtgc
    18721 gggcgcaggc gtttaatgac ctttgctcgg tgttcgttaa ggacgcgacg gttgtcgggc
    18781 tggacgatgg cgacatcgtt acggtgaaag aagcgaagga cgccctgctg cattgtgcgg
    18841 aacagcttcg caaaggaggc aaccagtgag caagattgac tatcaggcac tgcgtgatgc
    18901 ggcagtagca attgaaacag tagcaacgcc tcaaaaattg ctagcatttc gtatgaaagt
    18961 cacacctcag gttgtgctgg cactgctgga tgaacgggaa agaaaccagc aatacattaa
    19021 acagcgcgac caggagaacg aggagattgc gctaacggta ggaaagctgc gtgttgagct
    19081 tgaggaagta aaacaacacg ctgaaaaatt atccgaaacc aaggctgttc gtaaccaatg
    19141 gcggccagat atttgcccaa taaccggacg tacatttttc atgtggattg agcatccaac
    19201 attgggaaat gtgccgacat atggtggccc attagatagt tacaccattc caacaaagga
    19261 cggtgacggt gagttttcat gtgagcatta cgatcatgat tttggcggtt gggtagaaag
    19321 cgaatgtctt gggttatatc tgattgatga tagagaacaa tgcagggtct acgaactgga
    19381 ggaacgcgtt aaagagctgg aaacgcggga agttcatttg ccgactcgct acggccttcg
    19441 atatggacat ccgataaatg atgatgagcg ccacgtcatg atacctaaag aaaatggctg
    19501 ctggctttat ctggctgact tagaacatgc actacgtgtt gctggcattc gcatcaaagg
    19561 agggtgagaa agtgaatcga acaataatag tgacaactgt aatttcagtc cctgccaatg
    19621 ccccgcatca ggatattgat gattttgttg aaagtaaatt cgggcaagac aaagatatga
    19681 aaccggataa tttatgttat ggcgatgctg ccgaaataat tagccatcat tgggagtatg
    19741 gttcgcacta catgaattaa ttacatcata ttattaattt aatccttaac cggagggatt
    19801 tctgcaccct caaaacatcg ggaggccgcc cgaaagggcg gtaatgaatg gtcacattat
    19861 ttaggaaaaa atatccgcga aaaagtagag cgacagaatt tttgtttctc attctgttta
    19921 tcgtgttgat gataccgata tccccgctat tactgctctg gtttatcggg aaaataattg
    19981 aaccagttat tgagttgtat aacgacgtgg tatgggcgtc gttcaacaca ctgcacaata
    20041 aaattaaccc atataaggaa aactgaaatg gcaactttgc aggaattaat cgacctgacg
    20101 ccagaacagg aaaaagcgtg gaatcgtctt gtgaaggctg taaaggattt cagggcagcc
    20161 ggaggaaagt tttatagtgt cctagacacg ctgagcgcat acaacggcga gcacgttgcc
    20221 agcattgata acgataaggg ctaccacact gcaagcgtct atatgcctag cattgatgcg
    20281 cctgggttaa ccagttgggc tgatgattgg cacggcatca cgctgaaaga tggcgttgaa
    20341 gtggataagg actaacacat gacaacgttc accgacaaag aaatgattaa agaaatcaaa
    20401 gagcgaatcg gcagcctgga cgtgcgagat aatattgagc gccgtgctta tgaaattgcg
    20461 ttgactgcat tgaccactga accattcgcc actatcgaca cagtgggaat tgagctcgtt
    20521 aaatatggtt gtaacacgtt tatttgtccc gacaattcga tggagccggg aaatgtacca
    20581 ctatatatcg gcctgccacg aattgatcca gcaagccaga ctgccaagct gtcattccag
    20641 gaatggttgt cagaacaaaa agaaaaaata gacgttgatt gcggatgtgt aagcatcgaa
    20701 acgcttacgc actggatgaa atccgcttat gaggctggta actctccggt aactccggat
    20761 agttggataa gctgtagtga tcgaatgcct gaaaagggcc agaacgtgct tatttcggtg
    20821 aatttcgata gctctctggt tgaaccgcta atatgctccg cacactatac cggaagcacc
    20881 tttcggcgcg gagatgcaac gattaagccg ggtaatggta ttgagcaagc aactcactgg
    20941 atgccgctac cagaaccgcc gcaggaggtt aaccgtggct aacctgcaac ttgccgtcaa
    21001 aggtgaatac ttcgatgcca tgattcgcgg ggagaaaacg gaagagtatc gcctgtgtaa
    21061 tgactactgg aataagcgaa ttatgttccg ggaatatgac cgtctgatta tcacaaaggg
    21121 atatccgaag cgcgacgatt ccagccgcag aattgacgtc ccgtatgaag ggtatgaaat
    21181 caagacaatc acacatacac acttcggaga taaaccggta aaggtgttcg cgataaaggt
    21241 gaatatcggc agtgattgac ttcctcgcac tcgcggggat ttcttttatc tgaaaaggac
    21301 agaatacatg attaacaata ttgcaaatca gtcttcgttt ccggctgcaa caatcgacag
    21361 ccagatgctg ttgaaaatgg tcaatgaggc acggaaatta tgtagtgaaa agcccgttcg
    21421 caataatgat ttcattgcca gagttaagga tgagcttgaa ggagagacct acgaaatttt
    21481 cgtaggtcag aaaaacggcg cagaaataga agttataaca atgactttca agcaagccct
    21541 gcgagtagcc gcgcgtgagt cgaaggccgt tcgtcgttcg ctggtcgata aactggaatc
    21601 aatgcaacag cagttgcaac aaaaaaccac cacgaagaaa tcaccggatg gccttgaaga
    21661 attccgtaag gcacgcgcat tgaaaatgac cgtcgacaca atgaaggacc tcttcgactt
    21721 cctccctaac cttgcaccgg aagctaaaca ggtcgttgcc gccagcctgg ttaatcccgt
    21781 tgtaggcgct aacgtaatcc cgctaccgat gatcaatgaa cattactatt cggcgtcaga
    21841 agttggagca aaactcaaaa tatccgctaa caaggttggt cgcattgcca atacctacat
    21901 gctcaaaaca gaacaatacg ggaaatggtt catcgacaaa tccccgtatt cggataagca
    21961 ggtagaaagc tttcgataca ataatcgggg agtacgaaag attgaagaaa tcctggacgc
    22021 ggagaacaaa gcagagttcg gaacctgata tcctccactc tccgacgccg ccagaatgac
    22081 ggcgcggtgg ttaagcatga ggcgaaattt ctgccgggcg gtattttgtt gagagatgag
    22141 atacgtcaac ttctctttcc gctttttcaa ggctccatgc cgtctggaga cgaatccatg
    22201 aagacggagt tccacccagg acagcagcga gacgaacagc catttcagga gatattgtcg
    22261 tattacctga aaccagtcgc tgaacagtgg aaggcgcgac atcaagcgct cttgccagtt
    22321 ccctgattcc aatattctga tcttccagaa tatcagcaat aatttctccg ggatgagggg
    22381 ggttaaacat cgccattagt ggtaatcctc gtagttaaac gaagcggctc tggagttttt
    22441 tccctgtagc acgggcgaat tttttcaacg ttgcaaatga tggccttatt tacactcaat
    22501 ggttaattct ttcctgttaa atccctgttc ctttaaaatg tctttatatc catttttaca
    22561 tagggcattg gctttatcat agcatagtcc ccatggtgta gcggcaccac aggcaatgat
    22621 gtactgttcc ttttcatctc cgcgatttat tttcgtagtg gtagtgcatc ctgatgttgc
    22681 catgcagaaa attatcaaat aaaagaacct tgctatgatg ttcattatcg gatctctaat
    22741 aggtgctgca agtgacacta ttcccatacc tggagcagtt tgttagtgtg gcacttcata
    22801 tgccatgcag attactttcg ggcgcgaatc tcagagcgaa cctgtgaaat ggagtgccct
    22861 ttgcctaggt tctcacgaat agcgtcgagt ggcggcgcgg ctgagtgtat taaccacgct
    22921 tccattgctt tgtcgcgttc attcagtgca cgaagccctt cacgaataac ttcgctttct
    22981 gaagcgtatg caccggaagc cacacgggcg cgcaccatgt cagccatttc gttggttaat
    23041 gtaatgctga attgttgggt tgtacgcatg ttaaacctca ctgagtagga tagaacacta
    23101 ttcgatgata gcacgccact tgttgacgac aacacatatc cgggattata ttcttcgcac
    23161 ggtcgagttg accgtcggga ttcgaacccc ggatagaaac cgcgacagag acacgccgcg
    23221 agcgtgtttt ttattgtcgt atgcacgcgc acatctgaat tatggtgggg cgtatagggg
    23281 agctgaaaag ctcgccggtt ggtttcccgg tagttcgaac cctgtacgtc tcaccacccg
    23341 atgattcgaa cctaacggtg gtgatagttt agaaaccact cgagggcgtc ataatgacaa
    23401 ctcaagtttc tgttgaaaca ctctccacga ttacttacaa gcagatcccc gttatcacta
    23461 ccgaactttt ggcgcacctt tacggcacag aagctattcg tattcgccag aatcaccacg
    23521 aaaacaaagg tcgtttcatt gaggaaaaac acttcttcaa acttgaaggt gaaactttac
    23581 gtgagttcaa gcacagagta gcttttaact actctgtgaa aatcgcccgt aacgttcgct
    23641 ccctcatcct ctggacagaa cgcggcgcag cccgtcacgc aaaaatgctc gaaaccgatc
    23701 gggcgtggga agtgttcgaa aaactggaag actgctattt cagtcaaaaa atcccagaac
    23761 aacttcagct tccagaatca acgctatcta tcaactaccc tctctcgtgg ttttcagagc
    23821 accaccccta ctccatgatg agctatgttg atcgaaaaac tcttaacctg gacgtttctg
    23881 tactcttcga catgccaagc ccaactatgc gcatcctcaa cgagctacac agcaaaggct
    23941 ataacgttga cgccgctgtc gccgaattta acgccttcaa gcatctgacg gaagaaatgc
    24001 gccgtactct acaggatatt tcaagattgt cagatcgaaa ttcccgaaaa ggcttctcgt
    24061 taagcctgta acttccccta ccatccccga catcccgtcg gggttttcat atctggagac
    24121 aaaaatttga aacagatcgc tttctacagg cgttctggaa ggcccggtgc cttccgtggg
    24181 ttaaaagaac gcgtcacctg gatgattcag tctcgtggtc gtcctgttac tggcagtgaa
    24241 atagcagaga aatttggtgt ttcactttgc gagttcaaca aggtagctcg cggcctgaca
    24301 aagggcagta aggttgtgaa aataaaggct tcagaaccat tcaccacaga caccggaatc
    24361 gttgatcgct ttttctccct cgaatcaaat cctcgtcgtg acacacctcg ctcacgcaat
    24421 gccgttcctc cattcagtcg cagaagccgt gaacacgcag caaaaaactg tcgcgaggaa
    24481 tacgtgcaaa aggccgaacg ccgtcgccga ctgattaaag caggacttta cattgatgag
    24541 tttgaaaacg cgctatgacg aaaaaataca ccctcattta cgcagatcca ccatggacat
    24601 tccgcgacaa agcaaccgat ggtcaacgcg gtgcaagttt taaatatccg gtcatgagtc
    24661 ttctggatat ctgccgcctc ccggtatggg aactggcagc cgataattgc ctgttggcta
    24721 tgtggtgggt gcctacacaa ccacttgaag cattgaaggt tgtagaagcg tggggctttc
    24781 gtctggtgac gatgaaagga ttaacctgga acaaatgcgg gaaaagacag accgacaagc
    24841 tggtcatggg tatgggtagc accactcgcg ctaacagcga agactgcctt tttgcagtga
    24901 aaggaaatct gcccgaacgc attaacgccg gaataatcca gtcattcact gcaccgcgcc
    24961 ttgatcactc ccgcaagccg gatatggctc gagaaaaact tgtgcaactt cttggcgatg
    25021 ttccccggat agaactgttc gcccgccaca cctcgcatgg atttgatgta tggggtaacc
    25081 aatgcggcac accatccatt gagatggttc cgggtattgt taaatttctg gagaaaacca
    25141 atgagcgaaa aaacgacgtt gacaaaggca tcaccagttg aattaaggca gtgtctggaa
    25201 atcgcaaatc aacttgccag aagtggaata cgatttgttc caatcccgat tacagcagat
    25261 gcagaacttc atctgtttgg tgaaattctt tcccgaaagc tggatgaact ggaaaagctg
    25321 gtagaagaag ctgacacctc accaaccgta taacagcccc accgacatta aaatatcagg
    25381 agaaaaaaat gaacgcagtg ctcacagaat tgaacaaatt aggaaaagca tcagccgaaa
    25441 gtatttctaa aggtctcaat attgatttga atgacgttat tgacactcta tggaagttaa
    25501 aaaaccaggg ggtagtaact gtaaaaaatg gcatctggca ggcagttgca agggaagtgg
    25561 acaaaaaacc aaatatcgcc tcagtgcagc cagtgcagcc agtgcagcca gtgcagccag
    25621 tgcagcacaa cattataggt gacctgctac gtaaatcacg gaaagaagcg cgtcgcgccg
    25681 ggcggaaaca gaaacgatgg gagggtgcat gtaaggcgct gcaagaactg aataaatacc
    25741 gtgacttgat caacgaattg tcagagtgag cgaatcacag agagaatatt ttgagaaaca
    25801 acccgcgtac attaatattg tttttccttt ctctaatgac gggcggtatc ctgttattcg
    25861 caggaacaac attatttatt tttgttgccc ggctaactgc gaggataatg acatgaaaat
    25921 cacatttgaa agttacggtt taacagcaag tgtggttatc tccagctcta tattcgaaag
    25981 ccgcaaacat cagcatatcg ttgatgcagt gaaactcaaa gccccggaag ttaccgtcac
    26041 aacacatgga cttttcagga tacgaacgac gttgactaca acacacctct cggcatgccg
    26101 tgtttatgat atggcactca aggaatacaa ccagtgttcg tcctgatcca gcgcgggcag
    26161 tctttcgttg atgccaacaa ctatccggta gaaatatgca aggtaactct gactcaggtg
    26221 atctaccgaa ggctcgacgg cagaaccaga gccacttcaa ttggtgcatt taatgaagaa
    26281 tttgagcgaa tagagcacaa cgaactgcat atgattaaag cggaaattga gaaggaaaag
    26341 catattgcca gccttcgaaa aatgcgccgt acatcaatca actaacaacc gccttcgggc
    26401 ggtttattat gctgttgaaa aacatgtaga aaaggagagc atactatgga aatcaactta
    26461 atgaccgaaa aagaagtttc tgagttgtta caaaaaaggc gcacagcttt gtataatttg
    26521 cgaaaaaaac acggattccc tgatccagta cttactcatc cggcgcgata tagccgtcag
    26581 gccgttgaac aatggcttaa agccggagga attaaccgag ctgtttagcg tgccagaaaa
    26641 ttttatcagc atacaactca tacgcatctt tctgttccga caaccagtcg tgtttattat
    26701 acacggccat cactcctccc agttcatgcc ccagcatttt ttcagtgacg tggggcataa
    26761 ttccttcccc agataaattc gttaccagag aacgcctaaa atcatgcgtt ctccattcag
    26821 gaatatcaat tttcgccctg agttttttca tgtacaaatt agcagacgaa cggtcaatag
    26881 gtttatctat ttcctgccca gggaacagca catcaaatcc gatattaagc aatcgctcaa
    26941 tataaggctc aacctgagta aatatcggac gtcggataat attacccatc tttgaatgct
    27001 ctttaggggt tgtccagacc agatcttcca tgttgaactc actggctgtc gcgagacgta
    27061 gctcagaaag tctggccccc catagcaata gcaattgatg taacaccttg ttggaagtaa
    27121 ctacttttga gttctccagc gccagccaaa tttttgccag ttccgtatag gtaagtacac
    27181 gactccctac atctggtttt ttcccaatat tcttaacact aagcttgagt aattcgcatg
    27241 atgggattaa ctggcggctg atacaccagt taataactga acgcagttgt aacaacaaaa
    27301 cacgagcctt tttcttgttg aggctttctt gtttgtcaaa gaaacgagcc catgccgata
    27361 ctgggatact ggcaactggc gagccttcaa actgtgtgta catagtgttg tacacgacgg
    27421 atctgtaaag cgtctgtgta ttgggtttca gatcagtaac atacttatcc caccactgat
    27481 ccaggcattc ttttaatgtc agttcgccgt cactgggcgc aaaataattt ttagggttaa
    27541 ctcccttcat gtacaatgcg cgcatctcac cgacgataac acgcgcgtcc ttcagtgaag
    27601 tagacggata gcgcccgaca gtaagacgta ctggcttacc attccagcga taacggtact
    27661 gaaacgtgat agttcctgct ggagtaatcc gcacacttaa cccgtcacca tcggtgattt
    27721 cagcagggcc agagtagggt tttccgtaga ggcctcgtag tttggtgtcg ctcagtgcca
    27781 atgctttata tcctgtacac aagtttataa tgtattctgt actcaatatg tacgcattag
    27841 caagtgaaca aaacactttt caacgcaaaa gaagataatc aaccatgaac aaaaacgaac
    27901 ttacaccttg attttaaaac cataaagacg gtattatcag tcatatggtg aacaaccaaa
    27961 atcaatacgc aacaatgtcc tcttagttaa atggatataa cgagcccctc ctaagggcta
    28021 attgcaggtt cgattcctgc aggggacacc agataccctt caaacgatat ctaccttcac
    28081 cccgtaaaag ataagtttgg cagcacattt gccctatcta ctcatttttc ctgcaacagg
    28141 ttgaaatctc aacacggtca gaaaacgctg atgactaaac agccctgggc tgggcgatgt
    28201 aaccatcaca cacaatcctg atcgcgaaat atggcgtgac ttgatacttc actccacaat
    28261 gctttccttg atgaattcgc aggcccgtga tacacgggac aggtcgctga attacgacaa
    28321 tgccctggaa atcagcgagc cgtgtatccg gagtacattt gagcgactgt accagaacat
    28381 gaatgaggcg tttggattag gcgattatta gcagggctaa gcattttggt attattattt
    28441 tccggttgag ggatatagag ctatcgacaa caaccggaaa aagtttacgt ttatattgct
    28501 gaaggtactg gcgtttccat cactatttgc tcacgttttt tactcaggaa gaaaatgcca
    28561 aatagcaaca tcaggcagac aatacccgaa attgcgaaaa aaaccgtctg gtagcctgcg
    28621 tggtcaaaga gtatcccagt cggcgttgaa agcagcacaa tccccaacga actggcaatt
    28681 tgaaaaccaa tcagaaagat cgtcgacgac aggcgcttat caaaatttgc cacgctgtat
    28741 ttgaagacgg atatgacaca aagtggaacc tcaatggcat gtaacagctt cactaatgaa
    28801 ataatccagg ggttaacgaa tagcgcgcag gaaaggatac gcaacgccat aatcacaaca
    28861 ccgataagta atgcattttt tggccctacc cgattcacaa agaaaggaat aatcgccatg
    28921 cacagcgctt cgagtaccac ctggaatgag ttgagataac catacaggcg cgttcctaca
    28981 tcgtgtgatt cgaataaacc tgcataaaag acaggaaaga gttgttgatc aaaaatgtta
    29041 tagaaagacc acgtccccac aataaatatg acgaaaaccc agaagtttcg atccttgaaa
    29101 actgcgataa aatcctcttt ttttacccct cccacatccg ccgctacgca ctggtgttcc
    29161 tgatctttaa aacgcatgtt gatcatcata aatacagcgc caaatagaga gaccaaccag
    29221 aagttgatat ggggactgat actaaaaaat atgccagcaa agaatgcgcc aatagcatag
    29281 ccaaaagatc cccaggcgcg cgctgttcca tattcgaaat gaaaatttcg cgccattttt
    29341 tcggtgaagc tgtcaagcaa accgcatccc gccagatacc ccagaccaaa aaagagcgca
    29401 cccagaatta aacctacaga aaaattgctt tgcagtaacg gttcataaac gtaaatcata
    29461 aacggtccgg tcaagaccaa gatgaaactc atacaccaga tgagcggttt cttcagaccg
    29521 agtttatcct gaacgatgcc gtagaacatc ataaatagaa tgctggtaaa ctggttgacc
    29581 gaataaagtg tacctaattc cgtccctgtc aaccctagat gtcctttcag ccaaatagcg
    29641 tataatgacc accacagcga ccaggaaata aaaaagagaa atgagtaact ggatgcaaaa
    29701 cgatagtacg catttctgaa tggaatattc agtgccataa ttacctgcct gtcgttaaaa
    29761 aattcacgtc ctatttagag ataagagcga cttcgccgtt tacttctcac cattccagtt
    29821 cttgtcgaca tggcagcgct gtcatcgccc ctttcgccgt tactgcaagc gctccgcaac
    29881 gttgagcgag atcgataatt tgtcgcattt ctctttcatc tgtagataat cccgtagagg
    29941 acagacctgt gagtaacccg gcaacgaacg catctcccgc ccccgtgcta tcgacacaat
    30001 tcacagacat tccagcaaaa tggtgaactt gtcctcgata acagaccacc accccttctg
    30061 cacctttagt caccaacagc atggcgatct catattcttt tgccagcgcg catatctccc
    30121 gatcgttctg tgtttttcca ctgataagtc gccattcttc ttccgagagc ttgacgacat
    30181 ccgccagttg tagcgcctgc cgcaaacaca agcggagcaa atgctcgtct tgccatagat
    30241 cttcacgaat attgggatcg aagctgacaa aacctccggc atgccggatc gccgtcatcg
    30301 ctgtaaatgc gctggtacgc gaaggctcag cagacaacgc aattgaacag agatgtaacc
    30361 attcgccatg tcgccagcag ggcaagtctg tcgtctctaa aaaaagatcg gcactggggc
    30421 ggaccataaa cgtaaatgaa cgttcccctt gatcgttcag atcgacaagc accgcggatg
    30481 tccggtgcca ttcatcttgc ttcagatacg tgatatcgac tccctcagtt agcagcgttc
    30541 tttgcattaa cgcaccaaaa ggatcatccc ccacccgacc tataaaccca cttattccgc
    30601 ctaatctggc gattcccacc gcaacgttag ctggcgcacc gccaggacaa ggcagtagcc
    30661 gcccgtctga ttctggcaag agatctacga ccgcatcccc taaaacccat actttggctg
    30721 gcattttttt cccttaaatt catctgactt acgcatagtg ataaacctct ttttcgcaaa
    30781 atcgtcatgg atttactaaa acatgca
//
LOCUS       DAZKHK010000057        30468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKHK010000057 DAZKHK010000000
VERSION     DAZKHK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.057"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..448
                     /locus_tag="ACHOS5_002550"
     CDS             2..448
                     /locus_tag="ACHOS5_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFP9777349.1"
                     /translation="MAVCQRLITEKSYLSQEEIRRDLQNHGFDSISQSTVSRLLKLLG
                     VIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFILIHTVAGYGRAVARI
                     LDFHALPEILGVIAGSNIVWVAPRVVKRTALVHKQINYLLKLNIYS"
     gene            538..672
                     /gene="pyrL"
                     /locus_tag="ACHOS5_002551"
     CDS             538..672
                     /gene="pyrL"
                     /locus_tag="ACHOS5_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP9777350.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            676..1611
                     /gene="pyrB"
                     /locus_tag="ACHOS5_002552"
     CDS             676..1611
                     /gene="pyrB"
                     /locus_tag="ACHOS5_002552"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP9777351.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            1624..2085
                     /gene="pyrI"
                     /locus_tag="ACHOS5_002553"
     CDS             1624..2085
                     /gene="pyrI"
                     /locus_tag="ACHOS5_002553"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP9777352.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            2158..2544
                     /gene="ridA"
                     /locus_tag="ACHOS5_002554"
     CDS             2158..2544
                     /gene="ridA"
                     /locus_tag="ACHOS5_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP9777353.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(2750..5446)
                     /gene="mgtA"
                     /locus_tag="ACHOS5_002555"
     CDS             complement(2750..5446)
                     /gene="mgtA"
                     /locus_tag="ACHOS5_002555"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9777354.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(5587..5640)
                     /gene="mgtL"
                     /locus_tag="ACHOS5_002556"
     CDS             complement(5587..5640)
                     /gene="mgtL"
                     /locus_tag="ACHOS5_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP9777355.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            5825..6772
                     /gene="treR"
                     /locus_tag="ACHOS5_002557"
     CDS             5825..6772
                     /gene="treR"
                     /locus_tag="ACHOS5_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP9777356.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKTHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            6891..8312
                     /gene="treB"
                     /locus_tag="ACHOS5_002558"
     CDS             6891..8312
                     /gene="treB"
                     /locus_tag="ACHOS5_002558"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP9777357.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     CHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            8362..10017
                     /gene="treC"
                     /locus_tag="ACHOS5_002559"
     CDS             8362..10017
                     /gene="treC"
                     /locus_tag="ACHOS5_002559"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9777358.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLYK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFCHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLTILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCTMTLRPFEAVWWLQK"
     gene            10411..12549
                     /gene="nrdD"
                     /locus_tag="ACHOS5_002560"
     CDS             10411..12549
                     /gene="nrdD"
                     /locus_tag="ACHOS5_002560"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9777359.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            12707..13171
                     /gene="nrdG"
                     /locus_tag="ACHOS5_002561"
     CDS             12707..13171
                     /gene="nrdG"
                     /locus_tag="ACHOS5_002561"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP9777360.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(13216..13602)
                     /gene="cybC"
                     /locus_tag="ACHOS5_002562"
     CDS             complement(13216..13602)
                     /gene="cybC"
                     /locus_tag="ACHOS5_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9777361.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(13785..15137)
                     /gene="pmbA"
                     /locus_tag="ACHOS5_002563"
     CDS             complement(13785..15137)
                     /gene="pmbA"
                     /locus_tag="ACHOS5_002563"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP9777362.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGKVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            15231..15782
                     /gene="yjgA"
                     /locus_tag="ACHOS5_002564"
     CDS             15231..15782
                     /gene="yjgA"
                     /locus_tag="ACHOS5_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP9777363.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(15940..17313)
                     /gene="mpl"
                     /locus_tag="ACHOS5_002565"
     CDS             complement(15940..17313)
                     /gene="mpl"
                     /locus_tag="ACHOS5_002565"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP9777364.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            17489..18487
                     /gene="fbp"
                     /locus_tag="ACHOS5_002566"
     CDS             17489..18487
                     /gene="fbp"
                     /locus_tag="ACHOS5_002566"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP9777365.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(18520..19515)
                     /gene="yjfF"
                     /locus_tag="ACHOS5_002567"
     CDS             complement(18520..19515)
                     /gene="yjfF"
                     /locus_tag="ACHOS5_002567"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP9777366.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(19502..20524)
                     /gene="ytfT"
                     /locus_tag="ACHOS5_002568"
     CDS             complement(19502..20524)
                     /gene="ytfT"
                     /locus_tag="ACHOS5_002568"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP9777367.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(20538..22040)
                     /gene="ytfR"
                     /locus_tag="ACHOS5_002569"
     CDS             complement(20538..22040)
                     /gene="ytfR"
                     /locus_tag="ACHOS5_002569"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP9777368.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEIEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(22180..23136)
                     /gene="ytfQ"
                     /locus_tag="ACHOS5_002570"
     CDS             complement(22180..23136)
                     /gene="ytfQ"
                     /locus_tag="ACHOS5_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP9777369.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            23446..23976
                     /gene="ppa"
                     /locus_tag="ACHOS5_002571"
     CDS             23446..23976
                     /gene="ppa"
                     /locus_tag="ACHOS5_002571"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP9777370.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(24433..25428)
                     /locus_tag="ACHOS5_002572"
     CDS             complement(24433..25428)
                     /locus_tag="ACHOS5_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9777371.1"
                     /translation="MSIKKLADGKYCVDVRPAGSEGRRFRRRFPTRGEAAMYERHVLT
                     HYHDKDWVEKPIERKALSDLLELWWVYHGKNHRYGAMNRVRIEAVLRDMQELGINRSD
                     HLTRKNIIRYRLELMNRGIKQSTVNRYCAMMSGFFEKLIDVEEFVGDNPFHEIRKLTI
                     NQPEMAYLAHDDIPRLTALLSGDNLKAVLLSLATGGRWGEVANIKGEHIIGGKVIFME
                     TKNGSRRVIPIAKDLESLIKVKATGRLMNPSYAIVRSAIRTVRPDLPVGQSVHVLRHT
                     FATHFMINGGNIITLQRILGHSTIQQTMTYAHFAPDYLQDAVRFNPVAELSRFCP"
     gene            complement(25495..25794)
                     /locus_tag="ACHOS5_002573"
     CDS             complement(25495..25794)
                     /locus_tag="ACHOS5_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9777372.1"
                     /translation="MSTNVGEKIRIMRKSEMLTQEKMAEITGLTVAALRQYEQGRNEP
                     NLESTKKLLKSPVFRKYRDWFLFDEVEPKAGQVVPALAHIGRSESESPHSGKKTG"
     gene            25902..26327
                     /locus_tag="ACHOS5_002574"
     CDS             25902..26327
                     /locus_tag="ACHOS5_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP9777373.1"
                     /translation="MQEVTQNAPESPAETQCDQLTDAPETAHQKTGRKAPERQEIRLS
                     QNPSHLLSKEGFAMYIGKTVDAIVSMAKAGKLPAVYMADPLKPGGNAELYINRKAWDE
                     ACDRLTENAPQEWHEWENRLFLFKPAAGSRNKRISKKAA"
     gene            26640..26978
                     /locus_tag="ACHOS5_002575"
     CDS             26640..26978
                     /locus_tag="ACHOS5_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP9777374.1"
                     /translation="MTQRRNFRTGTERHAARFSTSASRSNTHYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRR"
     gene            26998..27240
                     /locus_tag="ACHOS5_002576"
     CDS             26998..27240
                     /locus_tag="ACHOS5_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP9777375.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFTEVAA"
     gene            27237..27362
                     /locus_tag="ACHOS5_002577"
     CDS             27237..27362
                     /locus_tag="ACHOS5_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777376.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            27442..27720
                     /locus_tag="ACHOS5_002578"
     CDS             27442..27720
                     /locus_tag="ACHOS5_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777377.1"
                     /translation="MSTNTPTEINKLFTDPAHIELTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            27732..27983
                     /locus_tag="ACHOS5_002579"
     CDS             27732..27983
                     /locus_tag="ACHOS5_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777378.1"
                     /translation="MNSIFTATRRTLLTHFTDAAGREFMVESYLITTTTPCPSDADYL
                     YIHLADGTQITAITSTVREVMTIRGAWKSETQAHGELRP"
     gene            27980..28192
                     /locus_tag="ACHOS5_002580"
     CDS             27980..28192
                     /locus_tag="ACHOS5_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777379.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            28219..28773
                     /locus_tag="ACHOS5_002581"
     CDS             28219..28773
                     /locus_tag="ACHOS5_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9777380.1"
                     /translation="MRLTPMGIPGKAPAHVKAWTQQEDRLLISLYQEHNTHQMAERIQ
                     RTRKAVMHRIRFLRNRGLIGRKRKAPLSTEAITFLIKNRHVKTAQEMALEVGCSECTV
                     RYTLRKRGYNLKKCGENHHCARYSDHLVELVTELRDIHNMTFCMIAEHINSTMQMHIN
                     DDAAFHLYHRRTATDAVLCELLPD"
     gene            28783..29163
                     /locus_tag="ACHOS5_002582"
                     /pseudo
     CDS             28783..29163
                     /locus_tag="ACHOS5_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            29094..29321
                     /locus_tag="ACHOS5_002583"
     CDS             29094..29321
                     /locus_tag="ACHOS5_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9777381.1"
                     /translation="MANRKQQRARAERLHIRTEINRRLHRAASVSFIMQSNALHRLND
                     GIAAGYYAAVFSYLAEDLAELQDLINQQHHH"
     gene            29390..29800
                     /locus_tag="ACHOS5_002584"
     CDS             29390..29800
                     /locus_tag="ACHOS5_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777382.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     HVTTYGDFSFTVDDRYRVDVKGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSVAV
                     KGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            29797..>30468
                     /locus_tag="ACHOS5_002585"
     CDS             29797..>30468
                     /locus_tag="ACHOS5_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9777383.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTDEGKRINSHLHHDEERRIRLEKLARHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSAALERTCDEVIEKTG
                     RGDDDL"
BASE COUNT         7699 a         8174 c         7574 g         7021 t
ORIGIN      
        1 actggcggtc tgtcagcgcc tgattaccga aaaaagttat ctttcacagg aggaaatccg
       61 ccgcgatttg caaaaccatg gctttgacag tatcagtcag tcaaccgttt cacgactact
      121 gaaattgctg ggagtcataa aaattagaaa tacaaaagga caaaaaattt attcggtgaa
      181 tcctcaatta cttcccacac ctgacgcagg tcgatccgtc gctgaaatgg tcctgagtgt
      241 cgagcataat ggtgaattta tccttatcca taccgtcgcc ggatatggtc gggcagtagc
      301 gcgaatcctt gatttccatg ctttgccaga aattcttggc gtgattgctg gcagcaatat
      361 cgtttgggtc gcaccacgtg tggtaaagcg tacagccctg gtgcacaagc aaattaatta
      421 cttattaaaa ttgaatatat attcatgata accgtttgcc ttgctttata actgcatcat
      481 cttcttgatc ctctctcaga gctgagtata atgccggaca atttgccggg aggatgtatg
      541 gttcagtgtg ttcgacattt tgtcttaccg cgtctgaaaa aagacgctgg cctgccgttt
      601 ttcttcccgt tgatcaccca ttcccagccc ctcaatcgag gggctttttt ttgcccaggc
      661 gtcaggagat aaaagatggc taatccgcta tatcagaaac atatcatttc cataaacgac
      721 cttagtcgcg atgaccttaa tctggtgctg gcgacagcgg cgaaactgaa agcgaacccg
      781 caaccagagc tgttgaagca caaagttatt gccagctgtt tcttcgaagc ctctacccgc
      841 acccgcctct ctttcgaaac ttccatgcac cgcctgggtg ccagcgtggt gggcttctcc
      901 gacagcgcca atacatcact gggtaaaaag ggcgaaacgc tggccgatac catttcggtt
      961 atcagcactt acgtcgatgc gatagtgatg cgtcatccgc aggaaggtgc agcgcgcctg
     1021 gccaccgagt tttccggcaa tgtaccggta ctgaatgccg gtgatggctc caaccaacat
     1081 ccgacgcaaa ccttgctgga cttattcacc attcaggaaa cccaggggcg tctggacaat
     1141 ctccacgtcg caatggttgg tgacctgaaa tatggccgca ccgttcactc cctgacccag
     1201 gcgctagcga agttcgacgg caaccgtttt tactttatcg cgccggatgc gctggcaatg
     1261 ccgcaataca ttctggatat gctcgatgaa aaagggatcg cctggagtct gcacagctct
     1321 attgaagaag tgatggcgga agtagacatc ctgtacatga cccgcgtgca aaaagagcgt
     1381 ctggacccgt ccgagtacgc caacgtgaaa gcgcagtttg ttcttcgcgc cagcgatctc
     1441 cacaacgcca aagccaatat gaaagtgctg catccgctgc cgcgtgttga tgagattgcg
     1501 acggatgtgg ataaaacgcc tcacgcctgg tacttccagc aagcaggcaa cgggattttc
     1561 gctcgccagg cgttactggc actggttctg aatcgcgatc tggtactgta aggggaaata
     1621 gagatgacac acgataataa attgcaggtt gaagctatta aacgcggcac ggtaattgac
     1681 catatccccg cccagatcgg ttttaagctg ttgagtctgt tcaagttgac cgaaacagat
     1741 cagcgcatca ccattggtct gaatttgcct tctggcgaga tgggccgcaa agatttgatc
     1801 aaaatcgaaa ataccttttt aagtgaggat caggttgatc agctggcgtt atatgcaccg
     1861 caagccacgg ttaaccgtat cgacaactat gaagtggttg gcaaatcgcg ccccagtttg
     1921 ccagagcgca tcgacaatgt gctggtctgc cctaacagca actgtatcag ccatgccgaa
     1981 ccggtttcat ccagctttgc cgtgcgaaaa cgcgccaatg atatcgcgct caaatgcaaa
     2041 tactgtgaaa aagagttttc tcataatgtg gtgctggcca attaattgcg gttggtaata
     2101 aaagtctggc tccctataat gagccagact ttttaccgct gtaataaagg agaaatcatg
     2161 agcaaaacta tcgcgacgga aaatgcaccg gcagctatcg gtccttacgt acagggcgtt
     2221 gatctgggca atatgatcat cacctccggt cagatcccgg taaatccgaa aacgggcgaa
     2281 gtcccggcag acgtcgctgc acaggcgcgt cagtcgctgg ataacgtaaa agcgatcgtc
     2341 gaagccgctg gcctgaaagt gggcgacatc gttaaaacta ccgtgtttgt gaaagatctg
     2401 aacgacttcg caaccgttaa tgctacctac gaagccttct tcaccgaaca caacgccacc
     2461 ttcccggcac gttcttgcgt tgaagtagcc cgtctgccga aagacgtgaa gattgagatc
     2521 gaagcgatcg ctgttcgtcg ctaatcttga tggaaatccg ggctatcatg cccggattaa
     2581 gtctgatgac aaaagcaaaa tcgcctgatg cgctacgctt atcaggccta cgtgattcct
     2641 gcaatttatt gaatttgtag accggattag gcggttacgc cgcatccggc atgaacaaag
     2701 ctcactttgt ctgcaatctg attccgggcc accatgccca gtttattctt tactgccaac
     2761 cgtaacgacg gctatagaaa cctttcacca actgggttaa cgtcatatac cctgccagaa
     2821 tcgcaaccag ccacgggaaa tagcttaacg gcagcgcctg cagttgcaga taactggcca
     2881 gcggtgaaaa cggcaatgcg atcccgacaa tcatcacgat cacggtcatg atcattaacg
     2941 gccacgatgc acagctctga ataaacggca cacggcgggt gcggatcata tgcacaatca
     3001 gcgtttgcga cagtaagccc accacaaacc agcccgactg gaacagcgtt tgcgtttccg
     3061 gcgtgttggc atggaatacc caccacatca ggcaaaacgt cagaatatcg aaaatcgagc
     3121 tgatcggtcc gaagaagacc ataaagcgcc ccagatccgc cggattccaa cgctgcggct
     3181 tttgaatttg ctcgtcgtcg acgttatcaa atgggatcgc cacctgtgac acatcgtaca
     3241 gcagattttg aataagcaag tgtagcggca acatcggcag gaagggcaag aaagcactcg
     3301 ccaccagcac gctgaacaca ttaccgaagt tagagctcgc cgtcattttg atgtatttca
     3361 gcatgttggc gaaagtgcga cgaccctcaa taaccccctc ttccagcacc atcaggcttt
     3421 tttccagtag gatgatatca gccgcttcac gggcaatatc taccgcgccg tccacagaaa
     3481 tgccgatatc cgcagcgcgt aaggcgggcg catcattaat accatcgccc ataaagccaa
     3541 ccacatgccc ttcgcgcttc agcaacgtta cgatgcgttc tttatgcatc ggcgtcagac
     3601 gagcaaacag cgtggtgcgc tgtgcgagat ttgccagttc gtcgtcagat agcgtttcaa
     3661 tatcactacc aatgaccacc tctcccgcgt ccagccccac ttcatggcac actttcgctg
     3721 caactaactc actgtcgcca gtaaggattt ttacggtaat tccactcgct tttaatgcct
     3781 tcagtgccgg agcggttgtc tcttttggcg gatcaaggaa agcaatatat ccttcgagga
     3841 tcaggtcgga ttcatccgcc cgctggtaat ctccttcacg cgctggcagg tatttcgtcg
     3901 ccaccgcaac cacgcgcagc ccctgacgat tcagcgtatc agtaacccgc ttaatcttac
     3961 gcagcatggt gtcatcgagc ggtacaatct cgccattgtg acgcacttgc gaacacacat
     4021 tgaggatttc ctgcaatgcg cctttgcaaa ccaactgatg gtgttcggta ttttctgcca
     4081 caaccaccga catccgacga cgctcgaaat caaatggaat ctcatcaatt ttttgccaac
     4141 ggctggccag cgagcgcgct gactcttcat ccgtaccttc gagcaccgcg gtatcaagca
     4201 ggtttttaag cccggtctgg taatggctgt tcaaccacgc gctatgcagc acgcgttcgc
     4261 tagttttacc ggagatatcg gtatgattct ccagcacaat tttatcctgt gtcagggtgc
     4321 cggttttatc agtgcacaga atatccattg cgccaaagtt ctgaatggca tccaaatgtt
     4381 tgacgatcac tttctgtttc gacaacttga ctgcgccacg cgccagcgtc gaggtaacaa
     4441 tcatcggcaa catttctggc gttaagccta ccgcgaccga aagcgcaaac agtgcagctt
     4501 cccaccagtc gcctttagtg taaccattga taagcaacac caccggagcc atcaccagca
     4561 taaagcgaat cagcagcata ctgacgcggc taatcccttg ctgaaaggca ttcggctcgc
     4621 tttcctgctc actaacacga cccgccagtt gaccaaacca ggtattggca cctgtggcaa
     4681 tcaccattgc ttgtgccgtg ccgctcacca cggtggtgcc cataaaacac agggtgtcgc
     4741 actccagcgg attgctgtgc tccggctggc gagtggtagc ggctttttct acgggcagag
     4801 actcaccggt taacgacgcc tgagcgacaa acagatcccg cgcctgcaag atacgtaaat
     4861 ctgccgggat catatccccc gccgccagtt ttataatatc gccgggcacc agttggtcta
     4921 tcgggatttc cagccagcca ttttcacctt tatcgttaat tacgcgcagt accgtcgcgg
     4981 tattactgac catcgctttc agggcatctg ccgctttggt ggaacgagct tcctgaataa
     5041 agttcagcaa cgtagaaata gcgaccatta gcgcgataac acctgcggca aataaatctt
     5101 ccgtggcgta agaaatagca ccgagaatgg taagcaaaat attaaagggg ttgcgatagc
     5161 agacccataa atgtacccac cacggcgacg gttgttgtgc gggtaattta ttttcaccat
     5221 gttgttcgcg ggcagattcc acttccgcca gatttaaccc ttccggatgg gtgtcgaacg
     5281 ttttccacaa ttcttcttcg ggcatcaccg ccattttcag gcaatgcgca cttaaagaag
     5341 gcgggaccgc cgcattcact gtctgctgcg cgccaggcaa tggatcacga tgaaccagac
     5401 gggaaggtaa atggcgaatg aaccgggtaa aaatttcttt aaacataagg agtccctccg
     5461 cactgtctga aacagtacag aaatgtcttc aggcgtggcg aagccttacc tgaaggtaat
     5521 aaagctgagc agggacgtta aaacgtcacg gccttacgca tccggtaacg ccgtgaaaag
     5581 acgggcttac cggaaaagtt ttaatctccg tcgagggaga ggcgtgggat caggttccat
     5641 ataacctccg gtaagtgaaa aaagcgccgc aaataataag gagaatccaa taatattcgt
     5701 agcatttatg gcgatataat acctgctggc aactaaacca acgataaacc agactttacc
     5761 cattgctgaa tgcacaggta aagttaggct caaataatta aacaacacgt tatgggacaa
     5821 caggatgcaa aatcggctga ccatcaaaga catcgcgcgc ttaagcggcg tggggaaatc
     5881 tacagtttcc cgggtgctga ataacgaaag cggcgtgagc cagcgcacgc gcgagcgtgt
     5941 tgaagcagtg atgaatcagc atggattttc cccttcccgc tccgcgcgcg ctatgcgtgg
     6001 gcaaagcgat aaagtggtcg ccatcattgt tacccgtctg gattcgttgt cagaaaatct
     6061 cgccgttcaa accatgctgc cagcgttcta tgaacaaggt tacgatccaa tcatgatgga
     6121 aagtcagttt tccccacaat tagttgccga acatttgggg gtgctgaaac ggcgtaatat
     6181 cgacggcgta gtgctgttcg gttttaccgg cataacagaa gaaatgttag cccactggca
     6241 gtcatcgctg gttctgctgg cgcgtgacgc aaaaggcttt gcttcggtct gttatgacga
     6301 cgaaggggca atcaagatcc tgatgcaacg gctgtatgac cagggacatc gtaatatcag
     6361 ctatctcggc gtgccgcata gtgacgtgac gaccggtaag cgacgtcacg aagcctacct
     6421 ggcgttctgc aaaacgcata aattgcatcc cgttgccgct ctgccagggc ttgctatgaa
     6481 gcaaggctat gagaacgtag caaaagtgat tacgcctgaa actaccgcgt tactgtgcgc
     6541 aaccgacacg ctggcacttg gcgcaagtaa atacctgcaa gagcaacgca tcgacacctt
     6601 gcaactggcg agcgtcggta atacaccgtt aatgaaattc ctccatccag agatcgtcac
     6661 cgtagatccc ggttatgccg aagctggacg ccaggcggcc tgccagttga tcgcgcaggt
     6721 aaccgggcgc agcgaaccgc aacaaatcat catccccgcc accctgtcct gatcgtttcc
     6781 tgaacgataa attgtgatct tcgctgcgtt tcgggaacgt tcccgttttt aaatttttcc
     6841 gcgcaatata ttctgcagcc aaccaaaaat gtcatctgcc atggggcttt atgatgagca
     6901 aaataaacca aacggatatc gatcggttga ttgaactggt cggcgggcgc ggcaatattg
     6961 cgacggtgag ccactgtatt actcgcctgc gctttgtcct caaccaaccg gccaatgcca
     7021 gaccgaaaga aattgagcaa ctccccatgg tgaaaggctg tttcaccaat gccgggcaat
     7081 ttcaggtggt gattggcacc aacgtgggtg attactatca agcactgatt gcgtcaaccg
     7141 gacaggcgca ggttgataaa gagcaggtaa aaaaagccgc ccggcagaat atgaaatgtc
     7201 atgagcagtt gatctctcat ttcgcggaga tcttcttccc gttgctgccc gcgttgatta
     7261 gtggtggttt gatcctcggt tttcgcaatg tgatcggcga tttgcccatg agcaacggtc
     7321 agacgctggc gcaaatgtac ccttccctgc aaacgatcta cgattttctg tggttgatcg
     7381 gtgaagcgat cttcttctac ctgccggtcg ggatttgctg gtcagcggtg aaaaaaatgg
     7441 gcggtacacc gatcctcggt atcgtgcttg gcgtaacgct ggtttctcca cagctgatga
     7501 acgcttatct gctcgggcag cagttgccgg aagtatggga ctttggcatg ttcagcatcg
     7561 ccaaagtggg ctatcaggcg caggtcatcc cggcactgtt agccggactg gcgctgggcg
     7621 ttattgaaac tcgtcttaag cgcatcgtgc cggattacct ctatctggtg gtggtgcccg
     7681 tctgttcgct gatcctcgcg gtgttcctcg cccatgcgct gattggtccg tttggtcgca
     7741 tgattggcga tggcgttgcc tttgcggtac gtcacctgat gaccggcagc tttgctccga
     7801 ttggcgcggc attgtttggc ttcctgtacg ccccgctggt gatcaccggc gtacaccaga
     7861 ccacgcttgc tattgatttg cagatgattc aaagcatggg tggcacgcca gtgtggccgc
     7921 tgattgcact gtcgaatatc gctcagggct ccgccgtgat aggtattatc atttccagcc
     7981 gcaagcacaa tgaacgcgag atctccgtgc ctgccgctat ctccgcctgg cttggagtca
     8041 ctgaacctgc tatgtacggc atcaacctga aatatcgttt cccgatgctg tgcgcgatga
     8101 ttggttctgg tctggcagga ttgctatgcg gcctgaacgg cgttatggcg aatggcatcg
     8161 gcgtaggcgg cctgccgggc atcctctcca ttcagccgag ctactggcag gtgtttgcgc
     8221 tggcaatggc tatcgccatc atcatcccga ttgtactcac ctcgtttatc tatcagcgga
     8281 aataccgcct gggcacgctg gacattgttt aattttcttc ggggcgcaat tgcgccccct
     8341 cgcatttgca ggaataacgt aatgactcat cttccccact ggtggcaaaa cggcgttatc
     8401 taccagattt atccaaagag ttttcaggac accacgggta gcggcaccgg cgatttacgt
     8461 ggcgttatcc aacgcctgga ctatctgtat aaactgggcg ttgatgccat ctggctaacc
     8521 cccttttatg tctctcccca ggtcgataac ggttacgacg tagcgaatta tacggcgatt
     8581 gatcccacct atggcacgct ggacgatttt gacgaactgg tgacacaggc aaaatcgcgt
     8641 gggattcgta tcattctcga tatggtgttt aaccatacct ctacccaaca tgcctggttt
     8701 cgcgaggcgc tgaacaaaga aagcccttac cgccagtttt atatctggcg cgatggagaa
     8761 ccagaaacgc caccgaacaa ctggcgttca aaattcggtg gtagtgcgtg gcgctggcat
     8821 gcagaaagcg aacagtacta tttgcatctc tttgcaccag aacaggcgga tctcaactgg
     8881 gagaatccag cggtacgcgc agagctgaaa aaagtctgtg agttctgggc cgatcgtggg
     8941 gtcgacgggt tgcgcctgga tgtggtgaat ctgatctcca aagatccgcg ttaccctgaa
     9001 gacctggatg gcgacgggcg tcgcttctac accgacgggc cacgagcaca cgagtttttg
     9061 cacgagatga accgcgatgt gtttacgcca cgcgggttaa tgaccgtagg tgaaatgtcc
     9121 tccaccagcc ttgagcattg ccagcgatac gcggcactga caggcagtga attgtcgatg
     9181 acctttaatt ttcatcacct gaaggtcgat tatcccggtg gtgaaaaatg gacgctggct
     9241 aaacctgact ttgtggcgtt gaaaacattg ttctgccact ggcaacaagg aatgcacaac
     9301 gtggcatgga atgccttgtt ctggtgtaac cacgatcagc cgcgcattgt ttctcgcttt
     9361 ggtgatgaag gtgaataccg cgtgcctgcg gcaaaaatgc tggcgatggt gctgcatggc
     9421 atgcagggaa cgccgtatat ctaccagggc gaagagattg gcatgaccaa cccacatttc
     9481 acgcgcatta ctgactatcg cgacgtggag agcctcaata tgtttgccga gctgcgcaac
     9541 gatgggcgtg atgccgacga gttattgaca atcctcgcca gtaaatcccg tgacaacagt
     9601 cgcacaccca tgcaatggac caacggcgat aatgccgggt ttacggctgg cgaaccgtgg
     9661 attggcctgg gcgataacta tcaggaaatc aacgtagaag cagcgctggc cgatgagtcc
     9721 tcggtgtttt acacctacca aaagttaatc gcactgcgta agcaagaagc cgtcctgaca
     9781 tggggcgatt accaggatct gctgccaaac agccctgtat tgtggtgcta tcgccgtgag
     9841 tggaaggggc aaaccttgct ggtcattgcc aaccttagcc gtgagaccca accctggcag
     9901 ccagggaaaa tgctcggcaa ctggcagctt gtgatgcata actacgaaga agcctcacca
     9961 caaccctgta ccatgacttt acggcctttt gaggctgtct ggtggttaca gaagtaaatc
    10021 ttcgctaagc cccggtaatg cctgtcattc cggggttttg cctgtcagta tgttgttttt
    10081 gttgatttta taatcaaaaa attcattaaa aaatttacat atcgctgtag cgcccgtcat
    10141 ccgtacgctc tgctttttac tttgagctac atcaaaaaaa gttcaaacat ccttgatgca
    10201 aagcactata tatagacttt aaaatgcgtc ccaacccaat atgttgtatt aatcaactat
    10261 aattgctact acagctcccc acgaaaaagg tgcggcgttg tggataagcg gatggcgatt
    10321 gcggaaagca ccggaaaacg aaacgaaaaa accggaaaac gcctttccca atttctgtgg
    10381 ataacctgtt cttaaaaata tggagcgatc atgacaccgc atgtgatgaa acgagacggc
    10441 tgcaaagtgc cgtttaaatc agagcgcatc aaagaagcga ttctgcgtgc agctaaagca
    10501 gcggaagtcg atgatgccga ttattgcgcc actgttgctg cggttgtcag cgagcagatg
    10561 cagggccgca accaggtgga tatcaatgag atccagaccg cagttgaaaa ccagctgatg
    10621 tcggggccat acaaacaact ggctcgtgct tacatcgagt accgtcacga tcgcgacatt
    10681 gaacgtgaaa aacgcggtcg cctgaaccag gagatccgtg gtctggtcga gcagaccaac
    10741 tcctcgttac tcaacgaaaa cgccaacaaa gacagcaagg tgattccaac ccagcgcgac
    10801 ctgctggccg ggatcgtggc taaacactat gcacgccagc acctgctgcc gcgtgacgta
    10861 gtgcaggcac atgaacgtgg tgatattcac tatcacgatc tcgattactc accgttcttc
    10921 ccgatgttca actgcatgtt gatcgacctg aaaggcatgc tgacccaggg ctttaaaatg
    10981 gggaacgccg agattgaacc gccgaaatcg atctctacgg caaccgcggt aactgcgcag
    11041 attattgctc aggtcgccag ccatatttat ggcggcacca ccattaaccg tatcgatgaa
    11101 gtgctggcac cgtttgtcac cgccagctac aacaaacatc gcaagaccgc agaagagtgg
    11161 aacatcccgg acgccgaagg ctacgctaac tctcgtacca tcaaagagtg ctacgatgcc
    11221 ttccagtcgc tggagtacga agtaaacacc ctgcacaccg ccaacggtca aacaccgttt
    11281 gtgacctttg gttttggtct gggcaccagt tgggagtcgc gcctgattca ggaatctatc
    11341 ctgcgtaacc gtatcgccgg cctcggtaaa aaccgtaaaa cggcggtgtt cccgaaactg
    11401 gtgtttgcga ttcgcgatgg cctgaaccat aaaaaaggcg atccgaacta cgacatcaaa
    11461 cagctggcgc tggagtgcgc aagcaagcgc atgtatccgg atatccttaa ctacgatcag
    11521 gtagtgaaag tcaccggttc gtttaaaacc ccgatgggct gccgcagctt cctcggcgtg
    11581 tgggaaaatg aaaacggcga gcagatccac gatggtcgta acaacctcgg cgtgatcagc
    11641 ctgaacctgc cgcgtattgc tctggaagca aaaggcgatg aagccacctt ctggaagctg
    11701 ctggatgaac gtctggtgtt ggcacgtaag gcgctgatga cccgtatcgc tcgtctcgaa
    11761 ggcgtgaaag cgcgcgtagc cccgatcctc tatatggaag gcgcttgtgg cgtacgtctg
    11821 aatgctgacg acgacgtttc tgaaatcttc aaaaacggtc gtgcgtctat ttcgctgggt
    11881 tacatcggca tccacgaaac cattaacgcg ctgttcggcg gcgagcatgt ttacgacaac
    11941 gagcagctgc gcgcgaaagg tattgcgatt gttgagcgtc tgcgtcaggc ggtggatcag
    12001 tggaaagaag aaactggcta cggtttcagc ctctacagta caccgagtga gaacctgtgc
    12061 gaccgcttct gccgtctcga taccgctgag tttggcgtgg tgccgggcgt gaccgataaa
    12121 ggttactaca ccaacagttt ccacctcgat gtggagaaga aggtgaaccc gtacgacaag
    12181 atcgactttg aagcgcctta cccgccactg gcgaacggtg gcttcatttg ctatggcgag
    12241 tatccaaaca ttcagcacaa cctgaaggcg ctggaagatg tctgggatta cagctatcag
    12301 catgtaccgt attacggcac caacacaccg atcgatgaat gctacgagtg tggctttacc
    12361 ggcgaatttg agtgcaccag caaaggcttc acttgtccga aatgtggtaa ccatgacacc
    12421 tcccgtgtgt cggtgactcg ccgcgtgtgc ggatatttag gtagcccgga tgcacgtccg
    12481 tttaacgctg gtaagcagga agaagttaag cgccgcgtta aacatttggg aaatgggcag
    12541 ataggttaat cttcactctg tgttaagcaa atgccggatg cggcgtaaac gccttatccg
    12601 gcctacattt ggcaacggct gtaggcctga taagacgcga cagcgtcgca tcaggcgttg
    12661 attaccggat gcagcctaca cgcggactga taagccaggt gaaataatga actatcatca
    12721 gtactatcct gtcgacatcg tcaacggccc cggcactcgc tgcaccctgt ttgtctccgg
    12781 gtgtgttcat gaatgccccg gttgctataa caaaagcacc tggcgggtaa attccggtca
    12841 gccatttacc aaagcaatgg aagaccagat cattaacgat ctgaatgaca ctcgcatcaa
    12901 acgccagggt atttccctct ccggcggcga tccgctgcat ccgcaaaacg tgccggatat
    12961 tctgaaactg gtacaacgca tccgcgccga gtgtccgggt aaagacatct gggtgtggac
    13021 aggctataaa ctcgacgaac tcaacgctgc gcaaatgcag gttgttgatt tgattaacgt
    13081 gctggtcgac ggcaaatttg tgcaggattt aaaagaccct tccctgatct ggcgcggcag
    13141 cagcaatcag gtggtgcatc atttgcgatg ataagttccc tgttacctga gtgcaggcaa
    13201 cagggaaata aagaattaac gatacttctg gtgataggcg ttgcgggtcg ttttcagttg
    13261 ctctgcggca gcctgcgctt cttttacttt accttcattt gccagcttca gcgcgtcgtc
    13321 aatctgaccg accagaatgt cgaaaccgtg gcggaagtct ttcatttccg ggctgtccgg
    13381 tgatttgcct tcaagcttcg gcggcgtcgc tttttgcgca tccagcgccg cagcgcgcat
    13441 cttcgttaac gcgtctttga cttgcgccgc gttatcggct ttttcaacca cttttaaatt
    13501 gtcgttgagg gtttccatat tgtcttccag atcagcggca aacgacgccg aactgaatac
    13561 caatgaggag actgcaagaa tagctaacag gcttttacgc attgctcact tcctttttta
    13621 tttacacttc atccttcaca ctgcctcatc gacggctacg ctcattcgcc cgagtcacgt
    13681 actacgagta cgctcccctg ctctcattca ctttccgtct cgatgcagtg cgaatgattt
    13741 tgtgtaattt tttagtttat agcgcggcag gtcgcgccag ttttttactg cccggcgatt
    13801 ttcatctccg gcaacagcac agaaccacac tgtatattac tgcgtgtttc aatatcgtta
    13861 ccgacggtga caatattgcg ccacatatct tttaaattac cagcgatggt gatttcgctc
    13921 accggatact gaatctcgcc attctctacc cagaagcccg ctgcaccacg ggaataatca
    13981 ccggtaatgg cactcacgcc ctggcccatc agctccgtca ccaccagccc ggtgcccatc
    14041 tctttgagca tttgttcgaa gcttagccct tgtccggcaa tccgccagtt gtgaataccg
    14101 cccgcatgtc cggtgctttt cagccccagt ttccgcgccg aatagctggt cagcagccac
    14161 tgagtcagga tgccatcttt gacgatatcg cggcgctcgg tgcgcacacc ttcgctgtcg
    14221 aatggtgtag atgccagccc tttcagcaga tgcggatgtt cttcaatagt cagccagtcc
    14281 ggcagaattt gtttacccag cgaatccagc aagaaggtcg atttacggta gaccgcacca
    14341 cccgctatcg cccccaccaa atgaccaaac aaccctgttg ccacttcatt ggcaaaaatc
    14401 accggcgctt tcatggtaga aagtttacgc ggtgacaggc gcgataaggt acggcgagca
    14461 cagtcggccc caacccactc tggcgtttgc agatcgctca tcgcacgccc aatggtgtag
    14521 gcataatcgc gctccatatc gccattttct tcggcgatta cacagctgga gagcgaatga
    14581 cgcgttgagc agtaaccctg caacatgccg tggctgttgc caaaaacttt gacaccgtag
    14641 tggctgttaa agctaccgcc ttcggtattg gtgatgcgtt tgtccgcctg caatgccgcc
    14701 tgttccgcgc gggccgccag ttcaatggct tcatccggag aaacgtccgc agggtggaac
    14761 aagtcaaggt ccggcgcgtc aaaagccagc agttctttgt ctgccacgcc tgcatacgga
    14821 tctggcgagg tataacgggc aatatccagc gccgcctgta cggtgcgggc aatggcctgc
    14881 gggcttaaat cggtagacga tgcgctacct ttacggttct ggtgataaac ggtgatcccc
    14941 agcgcgccat cgctattgaa ttcaacattc tccactttac cataacgcgt gcttacgcta
    15001 atgccggtgg tcttgctgac ggcaacttct gcaccgtccg atttgcctga ggccaactcc
    15061 agcgcagtcg aaactgcttc ttccagaatc ttgcgctgcg cttcaacttg agagattact
    15121 ttcattgcaa gtgccataat gtagagagtt tctctgaagt ctaacagaga accgtttttc
    15181 agtgcgcatc ttaactggta acattagcct ctttttttaa ggagcctgag atgactaagc
    15241 agcccgaaga ctggctcgac gacgttcccg gtgatgacat cgaagacgaa gacgatgaaa
    15301 ttatctgggt cagtaaaagt gaaattaaac gtgatgccga ggagctaaaa cgccttggcg
    15361 cggaaattgt tgatctgggg aaaaacgcgc tggataagat cccgttagat gcggatctgc
    15421 gcgcggctat tgagctcgcc cagcgtatta agatggaagg tcgtcgccgc cagttgcagc
    15481 tcatcggtaa gatgctgcgc cagcgcgatg ttgagccgat ccgtcaggcg ctggataagc
    15541 tgaaaaaccg tcacaaccag caggtggtgc tgttccataa actggaaaac ctgcgcgatc
    15601 gtctgatcga ccagggtgat gacgccatcg ctgaagtatt gaatctgtgg cccgatgccg
    15661 atcgtcagca actgcgaact ctgatccgca acgcgaagaa agagaaagaa gggaataagc
    15721 cgccgaaatc cgcgcgccag attttccagt atctgcgtga gttagcggaa aacgaagggt
    15781 aattttcgtt gttgatgtaa gtatggtttg ccggatacgg tgcacctgta ggcctgataa
    15841 gacgcatcaa gcgtcgcatc aggcactatg ctcaaatgcc ggatgcggcg tgaacgcctt
    15901 atccggccta cacacactct atcaggctga ggctgtgaat tactgcgcgg cttccgcttt
    15961 cttcgctaac ccatccagca gtttctgatg aattccacca aaaccgccgt tgctcatcac
    16021 cagaatatgg tcgccaggct gagcggtttt caccaccata tctgccagcg tatccacatc
    16081 gccgctccag tgtgcaggct gaacgcaggc ttctgccact tctgccacct gccacggaat
    16141 atgtgccggt tgcagcagga agacttcatc ggcacgacct aatgaaggtg ccagatcgtc
    16201 tttgcagatc cccattttca tggtgtttga gcgcggttcc agcacggcaa taatgcgcgc
    16261 cgtaccacca actttgccac gcagcgccgc cagcgttgcc agaatcgccg tcgggtgatg
    16321 ggcaaaatcg tcatataccg tgacgccatt cgcttcacca cgcaactcca gacggcgacg
    16381 agcgttaata aacgaaccca gcgcgttagc ggcatctgcc ggcgctacac caacatggcg
    16441 agccgccgca atcgccatca ggccattgtg catattatgt tcgcctacca gcgaccattt
    16501 cacttcgccc actttttcgc catccagcaa gacttcccat tcagaagcgt cagtggtcag
    16561 ctttttcgcc tgccagtgac cttgctcgcc caccagctcc tgctcgctcc aacagcccat
    16621 cgccatggtc tgtttcaggt tgatgtcgtt ttccggccag ataatacgcc cctgccccgg
    16681 aacgatacgc accagatggt ggaactgttt ctggatcgct ttcaggtcgt caaagatatc
    16741 ggcgtgatcg aactcaaggt tgttgaggat cagcgtacgc gggcagtaat ggacaaattt
    16801 agagcgtttg tcgaagaagg cgcagtcgta ctcatccgct tcgataacaa agaagtcgct
    16861 ttcgcccaga cgcgccgaaa cctcaaagtt ccccggcaca ccgccgatca caaatcccgg
    16921 tttgtaaccg cattgttcca gaatccaggt cgccattccc gccgtggtgg ttttgccgtg
    16981 tgtaccggca acggccagca cccagcggtc gcgcagcaca aaatcgtgca gccactgcgg
    17041 acctgacata taagggatgt ttttttccag taccgcttcc acacacggat ttccacgggt
    17101 catggcgtta ccaataatca ccagatccgg ctgtggatcg agctggctgg catcgtaacc
    17161 ctgaatcagt tcaatgcctt gcttctcaag taaggtgctc atcggcggat acacattggc
    17221 gtccgaaccc gttacttcat ggcctaactg gcgcgccagc atcgccagac cgcccataaa
    17281 cgtgccacaa attcctaaaa tatgaatgcg catacgtcac tatccttttt taatctggcg
    17341 ctcattttac gcatatgtcg ggcaagtgag aaacgcattt caggataatc cgtaatttgc
    17401 tggcgcgatt cacctgagcg caacattggg attatttgtt aagattgttg cggtcgcttt
    17461 actccataaa cattgcaggg aaagttttat gaaaacgtta ggtgaattta ttgtcgaaaa
    17521 gcagcacgag ttttctcatg ctaccggtga gctcactgct ttgctgtcgg caataaaact
    17581 gggcgccaag attatccatc gcgatatcaa caaagcagga ctggttgata tcctgggtgc
    17641 cagcggtgct gagaacgtgc agggcgaggt tcagcagaaa ctcgacctgt tcgctaatga
    17701 gaaactgaaa gccgcactga aagcacgcga tatcgttgcg ggcattgcct ctgaagaaga
    17761 agatgagatt gtcgtctttg aaggctgtga acacgcaaaa tacgtggtgc tgatggaccc
    17821 actggatggc tcgtccaaca tcgatgttaa cgtctctgtc ggtaccattt tctccatcta
    17881 ccgccgcgtt acgcctgttg gcacgccggt aacggaagaa gatttcctcc agcctggtaa
    17941 caaacaggtt gcggcaggtt acgtggtata cggctcctct actatgctgg tttacaccac
    18001 cggatgcggt gttcacgcct ttacttatga tccttcgctg ggtgttttct gcctgtgcca
    18061 ggaacggatg cgcttcccgg agaaaggcaa aacctactcc atcaacgaag gaaactacat
    18121 taagtttccg aacggggtga agaagtacat taaattctgc caggaagaag ataaatccac
    18181 caaccgcccg tatacctcac gttatatcgg ttcactggtc gcggatttcc accgtaacct
    18241 gctgaaaggc ggtatttatc tctacccaag caccgccagc cacccggacg gtaaactacg
    18301 tttactgtat gagtgcaacc cgatggcatt cctggcggaa caagcgggcg gtaaagcgag
    18361 cgatggcaaa gagcgtattc tggatattat cccggaaacc ctgcaccagc gccgttcgtt
    18421 ctttgtcggc aacgaccata tggttgaaga tgtcgaacgc tttatccgtg agttcccgga
    18481 cgcgtaagct cctgcgcctc tccacacgga gaggcgtttt tatcgctgcg caatgttgac
    18541 tcttgtcact ggcgaactct gacgattctc ccacagcacc gttaatccac gctgtaatgc
    18601 tataaaaata aacaacaaaa taccgatggc gattttcgtc caccaggaac tcagcgtgcc
    18661 atcaaagttt atgtaagtct gaatcagtcc ctgaatcgcc acgccaaaaa gcgtccctaa
    18721 taccgttcca acgccaccac tcaaaagcgt accgccaatt accactgagg cgatagcatc
    18781 cagttccaca cctacgcccg ccagtgcata tccggcctgg gtataaatcg agaagacaat
    18841 ccccgccagc gttgccagtc cggtggagag catataaatg cgaatagtgg tgctgcgagt
    18901 ggaaatcccc atcaggtttg ccgacgttgc gttaccgcca atggcgtata cctgattacc
    18961 aaaacgggta cgatgcgcga ggaatatgcc gataaccacc accgccagca tcagcagtcc
    19021 catcgcactt aagcgaccac cgccagggat tttccacgca aggcttgaga gcgtgtcata
    19081 aacaggatgg tttatcggga tcgactcttc cgaaacgaga tagctgacgc cacgcaaaaa
    19141 gaacattccc gcaagggtaa tgataaacgc cgggatcttc agggcgtcaa tcagaagccc
    19201 cataaatgcg ccgaaggcac agcccatcac caacaccagc ggaaacgcca gcaacggcga
    19261 gaggccgaaa tcgccaatca cttttgccag aaacacgccg gtaaaggcga tcaccgaacc
    19321 gacggagaga tcaatcccac cggagaggat cacaaaggtc ataccaacgg cgattatccc
    19381 aagaaaggca ttatcggtca ggatattgca gatcactctt gtggaagcaa aaccgggaaa
    19441 ctgggtcaga cagtagatat aacccaacac aaagacgccg atggtgatca tcagtggcaa
    19501 attacgtttt atcacggtta cgtacccctt taatcagact gataaagcgt tgcgactgga
    19561 caatcagcac gcaaagcacc accaccgctt tcaccacctg gttcatctcc ggcggaaagc
    19621 cagaaagcaa aattccggtg ttcattccct gaataatcag cgcccccacc accgaaagca
    19681 gcaggttaaa gcgcccgccc atcagcgatc cgccgccaat caccactgcg agaatggcgt
    19741 ccagctccag ccataatccg gcattgttgg catcggcacc gcgaatatcc gccgccacga
    19801 taatgcccgc aatcgccgca catagcccgc tcaacacgta ggtgagcatg acgatgatcc
    19861 gcgtgtttac cccggcattt tttgccgccc gaatgttgat accaacggct tcgataaaca
    19921 tccccagtgc cgttttgcgg gtcaacagcc agaacaggag aagcgtcagc accgcaataa
    19981 tgactggcgt tggcaggaac aacagcgatc cactgccgaa ccaggagaga tccggtgagt
    20041 taaatgtgac gatctgtccg gaggtgatca gttgcgccac gccgcgcccg gcgaccatca
    20101 ggatcagggt ggcgacaaac ggctgaattt tgaggatcgc taccagtatg ccgttccaca
    20161 atcccgccag gatgccagtg cccagggcgc ttaacaaaac aattggcagg ctgaatcccg
    20221 cgaccgtcat cgcagccgtt gtggctccgg cgatagccat caccgcccct acggagagat
    20281 caatcccacc agtggcgata accagcgtca tgccaatcgc cagtagcgca acgggggccg
    20341 cacggttaag aatgtcgata gggctaccga acaaacgccc atcctgaagc accacctgcc
    20401 agaaatgcgg ggccaccagg ctatcaacca gcagcaccag caacagtgcc gccagctgcg
    20461 gcattcctgt tggccagcga aaacgccttt tcggcggcgt agtgtccggg agagattgag
    20521 gcatcacact attctcctta cgccgcaatg gcgttcatga tcgccggaac ggaaagctct
    20581 gccagcggga tctccgctac ctgtttgcga tcgcgcatga taatcacccg gtcggcatag
    20641 cccaccagct cttccagctc ggaggagatc accagcagcg ccagcccatc ggcgcacagc
    20701 gtttcaatca ggcggatgat ctcggcgtgg gcaccaacat caattccgcg cgttggctcg
    20761 tcgagaatca gaaattgcgg tcgggtcagt agccaacgtg aaagcaacac tttttgctga
    20821 ttgccgccgg agagaaattc aatcggttgt tcggtggaag gtgtgcgaat gccaagctgg
    20881 cggataaagc gttcggcaat ctcttgctgc tctttgcggg aaataggtcg taaccagccg
    20941 cgctgggcct ggagagcaag gatgatattt tcccgcaccg aggcggcagc gatgatgcca
    21001 tcggttttcc tgtcttccgg gcagaagcca atgccaagaa ccgaagcctg atgtggcgag
    21061 cgtagcgttt gcagtttgcc tttgatcaac gccgtgccgc tatcagcagg tttgataccg
    21121 aagatcactt cggcggtttc ggtacgtcct gatcccagca agcctgccag accgacgatc
    21181 tcgccggggc gtacttcgag atcaaacggc gcgatcgttc cttttttgcc gtaattcttg
    21241 aacgcggcaa caggtttgtc gctcagcaat gttctcccgg cacgctgaag cgcgtgggtg
    21301 tccagttcac gccccagcat catttttacc agttcgatct gcggtagctc gcgcgtttcc
    21361 cgacagccta cgaaactgcc gttacgtaag acggtgatcc gatcgctaac ctgataaacc
    21421 tgatcgagaa aatgagtgac gaagatcaga ctgacgccgc gatcgcgaag ttgacgcatc
    21481 aggccaaaca gtaactctac ttcctgagtg tcgagactgg cggtgggttc atcgaggatc
    21541 agcactttgg cggagagatc gatagcccgg caaatggcga cgatttgctg catcgcgact
    21601 gaaaagcggt tgagcggttc gcgcacgtcg agggagaaac cgtaagatgc catcagttcg
    21661 gtggcgcgct tttctatctc tttacggcgt agaaggccga aacgtttggg ttcgcgacct
    21721 atgaatagat tatcagcgac cgacatattg ggtagcaggt tgacttcctg atagacggtg
    21781 ccgatgccga gttgttgtgc gtgggcggtg tttttcggtg agatagcctg gccttccagc
    21841 cagatggtgc cgcgatcggc gtggtataca ccggttaatg ctttgatgag cgtcgatttt
    21901 cccgccccgt tttcaccgag cagcgccatg atttcaccac ggcgcaggct gaaatcaacg
    21961 ttgtctaacg ctttgacacc ggggaaaaat ttacttaatc cttcggtgcg gaggatttcc
    22021 tggtgttggt cggtcgtcat ggtttccccc ggtgtaaagc gtgctcccag taggcatgat
    22081 aagacgcggt aagcgtcgca tcaggcttca gcgcaccacc gccggatgcg gcgcgagcgc
    22141 cttatccggc ctacgaatcg gcatcaggca tagcaaccct caatacccca tatttttctt
    22201 cttctctaac tcttcttttg cggtatcagg cagataaagg gtggatttgg tcagggttag
    22261 cttttcaggc atggtgccgt cttttttata tttctccagc gcatcgaaag caggacctgc
    22321 catgttcggc gtcagttcaa cgctggcgtt cgcttcgcca tcaatcatcg ctttgtagat
    22381 gtccggcacg ccgtcgatag agcctgtcag gatatctttg cccggtttca ggcccgcttc
    22441 tttaattgcc tgaattgcac caatcaccat gtcatcgtta tgggcgtaaa ccatgcagat
    22501 gtttttgccg ttgttttccg ctttgataaa gctctccatc acttctttgc ctttgctgcg
    22561 ggtgaagtca cctgactgcg agcggataat tttgatattt ggcgcattct taatggcttc
    22621 ggcaaagcct ttcttacggt caatggcgac gctggcccca acggtgccct gcagctccac
    22681 cacgttgcat ggtttgccat tcacttcttt taccagccag tcaccaatca acttgccttc
    22741 gaggatgttg tcggcggtga cagtggtcat atagagagat ttgtctttca catcgatgga
    22801 acggtcaagc aagaagaccg ggatttcggc atctttcgcc tcttttaata ccggttccca
    22861 acccgtcgcc accaccggag cgataaagat cgcatccacc ccttgtgcga cgaaggaacg
    22921 taccgcttta atctggtttt cctgcttttg ctgaccatcg gcaattttca gtgtgatacc
    22981 gcgtttttcg gcttcacttt tcgccacatt ggtttctgcg gcgcgccagc cggattccga
    23041 tccgacctgc gaaaatccta cggttaatgg agcggccaac gccatagacg acatggctgc
    23101 cgagactgca gagactacaa gtaagcgttt ccacataggg ttgtcctcgt cggggtaatt
    23161 attattggtg aaaagatgtt cgcgaaaaaa ctatagacaa ttcgttatgt aacggattgc
    23221 gttacatcac acttcaaaaa agtaaataaa acgttaatca caagtttgta atcgctttca
    23281 tctcactatg aaaaatgcgg ctacggttat ggattttcct gctctgtata ccgtcttaaa
    23341 actggcgaaa aaggaaaatg aagacgaaaa caagcgaaga cattcggcgc gagttggcta
    23401 taatactcgg cacttgtttg ccacatattt ttaaaggaaa cagacatgag cttactcaac
    23461 gtccctgcgg gtaaagatct gccggaagac atctacgttg ttattgagat cccggctaac
    23521 gcagatccaa tcaaatacga aatcgacaaa gagagcggcg cactgttcgt tgaccgcttc
    23581 atgtccaccg cgatgttcta tccgtgcaac tacggttaca tcaaccacac tctgtctctg
    23641 gacggtgacc cggttgacgt actggtcccg actccgtacc cgctgcagcc gggttctgtg
    23701 atccgctgcc gtccggttgg cgtactgaaa atgaccgacg aagccggtga agatgcgaaa
    23761 ctgattgctg ttccgcacac caagctgagc aaagaatacg atcacattaa agacgttaac
    23821 gatctgccag agctgctgaa agcgcaaatc gctcacttct tcgagcacta caaagacctc
    23881 gaaaaaggca agtgggttaa agttgaaggt tgggaaaacg cagaagccgc taaagctgaa
    23941 atcgttgctt ccttcgagcg cgccaagaat aaataagttc ttctggcgca ataaccctga
    24001 acaccgggct tcggttagta agggtttttt atgcccgcaa tttataaact ctctactcca
    24061 ctattattat tttcagcagt tgaaaggctt gaacggtaag aacaagctaa gctcgaccac
    24121 cattttgctg ttcatagcca cttgctggaa gttagccggc ctcactcata ctcaccgttc
    24181 aaagcgtgcc cccgttttac ccctgcacat ttgttaacgc cagcagagtt ttttatgcga
    24241 ttgcagatat ggaaagcggc ccggagagaa aagcaaaaga tgggaaagtt acaaaaaaaa
    24301 tcgcgagata catcgcataa aacaacgcca cccgaaggtg gcataagggg gtagcatgtg
    24361 ccgcactacc ccttgatata tcaaatagtt acaagttcaa agtggttcga aatcgtgcca
    24421 tttagtcccc gtttatgggc aaaatctgga caactccgca accggattga accttaccgc
    24481 gtcctggaga taatcaggtg caaagtgcgc ataggtcatt gtttgctgaa ttgtgctgtg
    24541 ccctaaaata cgctgaaggg taataatgtt ccctccattt atcatgaaat gagttgcgaa
    24601 tgtgtgccgt aaaacatgca cagactgacc gactggtaaa tcaggccgca cagtccttat
    24661 tgcacttctg actatcgcat aactggggtt cattaatctt ccagtcgctt tgactttaat
    24721 cagagattca agatctttag ctataggaat aacccgccgc gatccattct ttgtctccat
    24781 gaaaataacc ttcccaccaa ttatgtgttc acctttaata ttggcaactt caccccagcg
    24841 cccgcctgta gccaggctta acaatacagc cttaagatta tcgccagata aaagtgcggt
    24901 tagcctggga atatcatcat gtgcaagata agccatttcc ggctgattaa ttgttagctt
    24961 gcgtatctcg tgaaacgggt tatcacccac aaactcttca acatcaatca gcttttcaaa
    25021 gaaaccgctc atcattgcac aataacgatt taccgttgac tgcttaattc cgcgattcat
    25081 taattcaagg cgatatctga taatattttt tcgggttaag tggtcacttc tgttaatacc
    25141 aagctcttgc atatcacgaa gaaccgcttc aattctgacc cgattcatcg cgccataacg
    25201 atgattctta ccgtggtaca cccaccacag ttccagaaga tcgcttaacg ctttacgttc
    25261 aataggtttt tcgacccagt ccttatcgtg ataatgcgtc agaacatggc gttcatacat
    25321 cgccgcttca ccacgggtag gaaatcgccg ccgaaatctt cttccttcag atccagcagg
    25381 ccgcacgtcc acacagtatt tgccatcagc gagcttctta atcgacataa ggaagccccc
    25441 caatgaaaac atcatcttga tcacaatttt gagaaataaa ggccagatgg accgttaacc
    25501 agtcttcttg ccggagtggg gtgattccga ttcgcttcgc ccaatgtgtg cgagagccgg
    25561 taccacctgc ccggcctttg gttcgacctc atcgaacaaa aaccagtcac ggtatttacg
    25621 gaagacggga gacttgagca gcttttttgt ggattcaaga tttggctcat ttctcccctg
    25681 ttcatattga cgcagggccg ctaccgtgag ccctgtaatt tcagccattt tttcttgtgt
    25741 aagcatttca gacttacgca tgattctaat tttttcacct acgttagttg acatagtgag
    25801 ttaactctct tagtttatac atcaacaaac acacctagag acaatctagg cggctctaac
    25861 tggtgctaaa tagaaatagc accaataacg gaggatacca gatgcaagaa gtcactcaaa
    25921 acgcgcctga atcgcctgcc gaaacacagt gcgatcagtt gacagatgcg ccagaaaccg
    25981 cacaccaaaa gaccgggcgc aaagcccctg aacgccagga aatcaggctt tcacaaaacc
    26041 cttctcatct cctgtcaaaa gaagggtttg cgatgtatat cggcaagacc gttgacgcca
    26101 tcgtgagcat ggcaaaagca gggaaattac ccgctgtgta catggcggac cctctcaagc
    26161 ctggcggaaa cgccgaactc tacatcaacc ggaaagcgtg ggatgaagcc tgcgatcgac
    26221 tcactgaaaa cgcaccgcaa gaatggcacg aatgggaaaa ccgacttttt ttatttaagc
    26281 cagcagctgg ttcacggaat aaacgcatca gtaaaaaagc tgcgtgatat agaactggca
    26341 atcattggag gggcaagatg attaaacaca cggaaatcag acaaagaata aaaataagcg
    26401 acgacgcttc agtaataatc tgtaatcaga acttagatgc cattgttcgg gctggcaacg
    26461 atttgtattt ccattacccg acccattgca tcgttgtgac atttgaaaca gaagagtcct
    26521 taaaaaatgc aatggagcag acaagatttc agaatgaaac agtaataaga aaagacaact
    26581 gggcataaat gccaatacac cgttatttca attcttttaa cttaagcggg ttttccgata
    26641 tgacacagcg acgcaacttc cgcactggca cagaacgcca cgcagcccgt ttctcaacca
    26701 gtgcatcacg cagcaacaca cactacagcc tgcaggaaac gcacgccaca cctgacgggc
    26761 atctggttaa acaaaccggt aaacacacct ggctgattaa tgatgccgga attgtcattc
    26821 accggggcgc acgcaacccg attaccggaa atacctgtta ctccctgact cgcggagatg
    26881 agcagttcgg gcaggatttc accctgcacg aagcattaag aacggcagac cggttaatca
    26941 gcggtcgcag ttttcagcac ccggaacatc gccgctgata aataaaagga ttcagccatg
    27001 atcgaagcct acaaaatact tattaataat gcgttgcagc gttatcactt cgaactcgca
    27061 aaccggggtg taaccaggga cgaaggactt cgtaactcac tgcggggcgc aattatggga
    27121 ctgtataact gcgcactggg cagcgatgac cgcgaagcac tggaagaatt aaaagttatt
    27181 gtggccgaac tgtttgaagg tgaaatcgtt gagccttatt tcacagaggt agcagcatga
    27241 gcgtatttct ttcatgggtg attctgatta tttccgtgct tatggcgatt ggcattatgc
    27301 gaattatcga agcgctgaaa aatatcgaat ctttatttac tggcgaagaa cgccgtaaat
    27361 aaataaccaa ccggacagaa aacattaaaa ccatccgcat ctgcggaggg cttcactcaa
    27421 tccagaaacg gagaaacagc tatgagcacc aatacaccta cagaaataaa caaactattt
    27481 accgacccgg cgcatattga attgacgcaa aaaacgctga ctgaaataac caatgtactg
    27541 gatgaaagaa tatcagaaat agataaagac aagcattttg ctacttacat ggccctgcaa
    27601 atgcaaagca tggcaatggt gacagcaaaa cagaccatca gtgaactcta tatgaaaaac
    27661 ctgcgcctcg agcgcgaact ggcagaactc cgggcacagt ccggccagtt ttcagcgtga
    27721 ggggcaggac gatgaacagc atatttaccg ccacaagacg aaccctgctg acgcatttca
    27781 ccgatgccgc aggccgcgag ttcatggtgg aaagttattt gatcacaacg accacgccgt
    27841 gcccgtcaga cgctgattat ctctacatcc atctggctga cggcacacaa atcaccgcga
    27901 ttaccagtac cgttcgcgaa gtaatgacca tcagaggggc atggaaatca gaaacacaag
    27961 cccacggaga gctgcggcca tgatcagcaa gtacaccacc aacgcacgta aacccggtaa
    28021 agacgcacgc caccacatcg ccgtactccg caatctgtta ctggaggaaa tcgacgactt
    28081 cacggcaacg ctgagcggtc cgggcgcacc agtaacacca ggtgagatcc accgggacct
    28141 taaacgccgt attgaaaacg ccattgatta caccctgaac ccgaccgaat aaccggaaca
    28201 gtaacggagg ttatcgcgat gagacttaca cctatgggaa taccgggcaa agccccggca
    28261 catgtgaagg cgtggacaca gcaggaagat cggttactca tctcgcttta tcaggaacac
    28321 aacacacatc agatggctga acgaattcag cgaacccgta aagcagttat gcaccggatt
    28381 cgttttttac gtaatcgtgg gctgattggc cgcaagagaa aagcaccact gagtacagaa
    28441 gccataacct ttcttattaa aaaccgtcat gtaaaaaccg cacaggaaat ggctctggaa
    28501 gtgggatgca gtgagtgcac agtgaggtac acactgcgta aacggggtta caacctgaaa
    28561 aaatgcggtg agaaccacca ttgcgccaga tacagcgatc atctggttga gctggtcacg
    28621 gagctacgcg acatacacaa catgacgttc tgcatgatcg ctgaacacat caacagcacg
    28681 atgcagatgc acattaacga cgatgcggcg tttcatctct accaccgccg caccgcaact
    28741 gacgcagtgc tctgtgaact gttaccggac tgagtgatca tcatgcacac acaaaaaaac
    28801 cgcttgccag cgttcgcctg gtcgggttac agttttcccg cacctcataa aacgggtgcc
    28861 gggattgcaa cccccgctga ctacacaagc gcacaaccgc gccagcggtt tttttgtgcg
    28921 taccgcatcg tcacatcttt ttcgcaccga aattatggtg gggcgtacag ggccgacttc
    28981 ggtcgggccg gggtcttgtg tagccggtgg ttgcaaccct gtacgtctca ccacccagaa
    29041 ttgcaacctc tggatggtga gttttcaaaa cttactacac aagaggccac accatggcaa
    29101 accgcaagca acagcgcgcc cgcgctgagc gtcttcacat ccggactgaa atcaaccgca
    29161 gactccatcg ggctgcatct gtctccttca tcatgcagtc aaacgcatta catcgactta
    29221 acgacggtat cgctgccggg tattacgcgg cagtcttcag ctatctggcc gaagacctgg
    29281 cagaactgca ggaccttatc aaccagcaac accaccacta aacaacatcc tgaagccatc
    29341 ccgcacccgt gcgcgggagg cttccgcacg tctgttaccg gaggttctca tgaagagcgt
    29401 taagggactt tcagatattc aggatttttt taactggatg tgggcagtgg atccccggct
    29461 ggcttcccgc ctgaacgact ggcacgacta ttaccgccgc gcctgtaaac aggccgccag
    29521 acacgtcacc acatacgggg atttctcctt caccgttgac gaccgttacc gcgtggacgt
    29581 gaaaggtgat ggttttggtt tatttcacat caacggaacc gacagcaccg ccatcagttg
    29641 gtcgtcgtca gagatgacgc ttgttctgga aatgacaaac cacagcgtgg ccgtgaaagg
    29701 tatatacacc gcagaagact ttatcggtga ataccagcgc cttctcgtgc tgtaccgcgc
    29761 ggcgtttcag gcggcgactg ccggggagtg cgacgcatga gcgaccataa cgcacaactg
    29821 gcgtacccgt ggaacgcccc gctaccggtt atcgatcctg aaacgttcgg cagagcgcaa
    29881 ccaaccgcat tacgccaggc cattcagagc tacatcaacg aagacattcg cattgatgca
    29941 agactggatg aagagaccgt cgacttcctg acaaacacag acgaaggcaa gcgaattaat
    30001 tcgcatcttc accacgacga agagcgtcgg atcaggctgg aaaaactggc gcggcacaaa
    30061 cgcgaaaatc cacccacgct ggtatctgaa gccatggcag agctgcgcgc cctgccgccg
    30121 tttcttgccg gtccgctcat ccgtgacctg gcccgcctga gccgcaaaca ggagagcgca
    30181 cgccgggaag ggcttaagaa taacagcgca tatgcggcgg ataacttcgt tcgtcacggg
    30241 ctgcgtgccc gcctgaaacg tatcgcccgt gtgaatgaac gctttgccac cccggccttt
    30301 aaggccaccg ccgcgcgcga acggctggat gaactgctta tgctgccgca gctgagccgt
    30361 gacgaaatcc agcgccttgc caccctgacc gccaccgcgt tttccgccgc actggagcgc
    30421 acatgtgatg aggtcatcga aaagaccggc aggggtgacg atgacctg
//
LOCUS       DAZKHK010000058        30302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKHK010000058 DAZKHK010000000
VERSION     DAZKHK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.058"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..604
                     /locus_tag="ACHOS5_002586"
     CDS             <1..604
                     /locus_tag="ACHOS5_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9777384.1"
                     /translation="EKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPE
                     GDYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGR
                     DALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERI
                     HATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            922..1437
                     /gene="ehxC"
                     /locus_tag="ACHOS5_002587"
                     /gene_synonym="hlyC"
     CDS             922..1437
                     /gene="ehxC"
                     /locus_tag="ACHOS5_002587"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP9777385.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNMEL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            1439..4432
                     /gene="ehxA"
                     /locus_tag="ACHOS5_002588"
     CDS             1439..4432
                     /gene="ehxA"
                     /locus_tag="ACHOS5_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP9777386.1"
                     /translation="MTVNKIKNIFNNAASTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVIGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQSFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGILEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSNLIQFVEKDNKHYQARIISELGDKDDIVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGVDNHLVEVI
                     TDKDGREIKADKIPHNNERSGYIKASNIASEKNIVNITSVADDINKIISSVSGFDSGD
                     ERLASLYNLSLHQNNTHSTTLTTTV"
     gene            4482..6602
                     /gene="ehxB"
                     /locus_tag="ACHOS5_002589"
                     /gene_synonym="hlyB"
     CDS             4482..6602
                     /gene="ehxB"
                     /locus_tag="ACHOS5_002589"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP9777387.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELTKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PIEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            6606..8045
                     /gene="ehxD"
                     /locus_tag="ACHOS5_002590"
     CDS             6606..8045
                     /gene="ehxD"
                     /locus_tag="ACHOS5_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP9777388.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGVFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKFPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
     gene            8172..8333
                     /locus_tag="ACHOS5_002591"
     CDS             8172..8333
                     /locus_tag="ACHOS5_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9777389.1"
                     /translation="MDIIKLLNKVIIYQQQNEQSYPAQEHLLLQLCMRVTKKLTDNIN
                     SSLKGGWYK"
     gene            8344..8712
                     /locus_tag="ACHOS5_002592"
     CDS             8344..8712
                     /locus_tag="ACHOS5_002592"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9777390.1"
                     /translation="MVLALLSSADNFFLSPTELSEKLDISRTNITRVFDSLEKFGFIR
                     RMESKEDRRSKNIYLTPDGDLLLQKITRIYGRYLKKYGATLQMIKSRYLSNQQKDVIS
                     KYFSVILHVIMYINPEFLFL"
     gene            8795..9016
                     /locus_tag="ACHOS5_002593"
     CDS             8795..9016
                     /locus_tag="ACHOS5_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFP9777391.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSAFNCHLHLSIDRSLASWYYPKND
                     KTALHPLHYWLHEGSEQKKNPFPIWFYPR"
     gene            9137..9877
                     /locus_tag="ACHOS5_002594"
     CDS             9137..9877
                     /locus_tag="ACHOS5_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9777392.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRVRRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRCEPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(10120..11097)
                     /locus_tag="ACHOS5_002595"
     CDS             complement(10120..11097)
                     /locus_tag="ACHOS5_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9777393.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENQLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(11870..12145)
                     /locus_tag="ACHOS5_002596"
                     /pseudo
     CDS             complement(11870..12145)
                     /locus_tag="ACHOS5_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12174..13277
                     /locus_tag="ACHOS5_002597"
                     /pseudo
     CDS             12174..13277
                     /locus_tag="ACHOS5_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
     gene            complement(13299..13634)
                     /locus_tag="ACHOS5_002598"
     CDS             complement(13299..13634)
                     /locus_tag="ACHOS5_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1A immunity protein"
                     /protein_id="HFP9777394.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            13763..14109
                     /locus_tag="ACHOS5_002599"
                     /pseudo
     CDS             13763..14109
                     /locus_tag="ACHOS5_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            complement(14129..14719)
                     /locus_tag="ACHOS5_002600"
     CDS             complement(14129..14719)
                     /locus_tag="ACHOS5_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777395.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAS"
     gene            complement(14719..14976)
                     /locus_tag="ACHOS5_002601"
     CDS             complement(14719..14976)
                     /locus_tag="ACHOS5_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777396.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            15544..15957
                     /locus_tag="ACHOS5_002602"
     CDS             15544..15957
                     /locus_tag="ACHOS5_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777397.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLNGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVRTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            15959..16738
                     /locus_tag="ACHOS5_002603"
     CDS             15959..16738
                     /locus_tag="ACHOS5_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9777398.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            16918..17562
                     /locus_tag="ACHOS5_002604"
     CDS             16918..17562
                     /locus_tag="ACHOS5_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9777399.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            17649..17957
                     /locus_tag="ACHOS5_002605"
     CDS             17649..17957
                     /locus_tag="ACHOS5_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFP9777400.1"
                     /translation="MSKVKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEVV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            18371..19351
                     /locus_tag="ACHOS5_002606"
     CDS             18371..19351
                     /locus_tag="ACHOS5_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP9777401.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            19344..19760
                     /locus_tag="ACHOS5_002607"
     CDS             19344..19760
                     /locus_tag="ACHOS5_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP9777402.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(19762..21036)
                     /locus_tag="ACHOS5_002608"
     CDS             complement(19762..21036)
                     /locus_tag="ACHOS5_002608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP9777403.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEIWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(21036..21473)
                     /gene="umuD"
                     /locus_tag="ACHOS5_002609"
     CDS             complement(21036..21473)
                     /gene="umuD"
                     /locus_tag="ACHOS5_002609"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9777404.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(21470..21718)
                     /locus_tag="ACHOS5_002610"
     CDS             complement(21470..21718)
                     /locus_tag="ACHOS5_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9777405.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERIMSLLEEIWQDDSWLPAA"
     gene            22134..23036
                     /locus_tag="ACHOS5_002611"
     CDS             22134..23036
                     /locus_tag="ACHOS5_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9777406.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWKGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            23033..23343
                     /locus_tag="ACHOS5_002612"
                     /pseudo
     CDS             23033..23343
                     /locus_tag="ACHOS5_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23419..24102
                     /locus_tag="ACHOS5_002613"
     CDS             23419..24102
                     /locus_tag="ACHOS5_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP9777407.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWNRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            24103..24324
                     /locus_tag="ACHOS5_002614"
     CDS             24103..24324
                     /locus_tag="ACHOS5_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777408.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            24284..24770
                     /locus_tag="ACHOS5_002615"
                     /pseudo
     CDS             24284..24770
                     /locus_tag="ACHOS5_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            24815..25585
                     /locus_tag="ACHOS5_002616"
     CDS             24815..25585
                     /locus_tag="ACHOS5_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777409.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDVSRLATLLISRHDGVLPPVLASAMQKLTGTVAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            25999..26424
                     /locus_tag="ACHOS5_002617"
     CDS             25999..26424
                     /locus_tag="ACHOS5_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9777410.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            26471..26890
                     /locus_tag="ACHOS5_002618"
     CDS             26471..26890
                     /locus_tag="ACHOS5_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP9777411.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            26890..27081
                     /locus_tag="ACHOS5_002619"
     CDS             26890..27081
                     /locus_tag="ACHOS5_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777412.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(27060..27531)
                     /locus_tag="ACHOS5_002620"
                     /pseudo
     CDS             complement(27060..27531)
                     /locus_tag="ACHOS5_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27618..27824
                     /locus_tag="ACHOS5_002621"
     CDS             27618..27824
                     /locus_tag="ACHOS5_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9777413.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKRVKFIP
                     QGIQQQVPAKRQNRGSRNSTGDVL"
     gene            28046..28276
                     /locus_tag="ACHOS5_002622"
     CDS             28046..28276
                     /locus_tag="ACHOS5_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777414.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            28328..29689
                     /locus_tag="ACHOS5_002623"
     CDS             28328..29689
                     /locus_tag="ACHOS5_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP9777415.1"
                     /translation="MTLSAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPEQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAKSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTANYSFLGYSGTMKVTPDRITDYKAPSAEDAAVARQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            29735..30298
                     /locus_tag="ACHOS5_002624"
     CDS             29735..30298
                     /locus_tag="ACHOS5_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP9777416.1"
                     /translation="MHSQLKERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRHVAPGAMCDAVEINSGLVRYLRDNFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTRVPTMIIRLRA"
BASE COUNT         7904 a         6750 c         7769 g         7879 t
ORIGIN      
        1 agaaaagtta cgtcagataa aggcgggtat gaataaggac cagatttatc agctggcggg
       61 gagaccacac tttgctgaag ggatgtctgg tgtaagggaa tgggattacc tgttcaactt
      121 taccggaccg gagggggatt atacctgcca gtacaagata ctgtttgacc ggaacatgga
      181 tgcccgctct ttctactgga agccggaaaa ctgtctgccg gaggcagaga cagcagaata
      241 cagggagtca gtccccggtg atatattatt tgattttgat tcatggactc tgacagagag
      301 ggggcgtgat gcgctttccc gtgtggcaga gaagtttcag gcgggtcatg ttgtcggggt
      361 gaagatagaa ggatttacgg accatcttgg cgggcaggcg tataaccggc tcctgtcaga
      421 gaaacgggcg gaatcagtca ggggatggtt tattgcccgg ggattcaggg ctgaaaggat
      481 acatgcaacg ggccgtggat atgatgcacc agtggtgttc tgcccggatg tggagggcga
      541 agcactgaag gaatgtctgc gccccaaccg cagggttgaa ataaccggac agagcgttcg
      601 ttaatatgca gtgtagcccc ggaatgtccg gggctttttt attaagaggt gggttttact
      661 ttgctgcatc ctgtttgata tttctttgat gagaagtttg tcgcagggtg attcatctga
      721 atgttataat ataacttatc tgggtgtgca taagaataca tggctaatgc attactgttt
      781 ctaatgttgt tttttttgta ggagggattc tccttgcggt agctttttgt ttttagatgc
      841 ttattgctta aaggatataa tttttatgct tttatattga tttctttaca tttactgagt
      901 tttcttattt atttaaatat tatgaaatct aatgcttttg acgtcatggg gaaggttgca
      961 tggctatggg cctgttctcc tctgcataaa aaatggccat tgtctgtttt tgctattaat
     1021 gtcatacctg ctattcagac aaatcaattt gctctcttaa taaaggatga gcttcctgta
     1081 gctttttgta gctgggccag cttagatctg gagtgtgagg taaaatatat aaatgatgta
     1141 acatctttgt atgcaaaaga ctggttgtcg ggagaaagaa agtggtttat tgactggata
     1201 gctccatttg gacacaacat ggagttatat aaacacatga ggaaaaagta tccttatgag
     1261 ttattcagag caataaggtt agatgagtac agtaaaacag ggaaaatagc tgaatttcat
     1321 ggagggggga tagataaaaa actggcatca aaaatattca ggcaatacca tcatgaactt
     1381 atgtctgaag taaaaaacag acaagatttt aattttaata ttgaaaaaga aaactaatat
     1441 gacagtaaat aaaataaaga acattttcaa caatgcggca tcgactacaa aatcagcatt
     1501 caatacagca tcatcaagcg tacgttccgc tggaaaaaaa ctcatattat taatacctga
     1561 taattatgaa gctcagggcg tgggtattaa tgagttggtc aaagctgctg atgatcttgg
     1621 aatagaaata caccgtactg aacgagatga tacagcgatt gcaaaccagt tttttggtac
     1681 agcagaaaaa gttataggat taactgaacg tggtgttgca atattcgcac cacaacttga
     1741 caaacttctg cagaagtatc agaaagttgg gagtaaaata ggaggaaccg ctgaaaatgt
     1801 aggtaataat ctgggaaaag ccggaacagt tctctcagca ctacagagtt ttacggggat
     1861 tgctttatca ggcatggctc ttgatgaatt gctgagaaaa caacgggaag gagaggatat
     1921 aagtcagaat gatattgcca aaagtagtat tgaacttatt aatcagcttg tagatacagt
     1981 atcaagtata aacagtaccg ttgattcatt ttctgagcag cttaaccagc ttggctcatt
     2041 tttatccagt aaacctcgct taagttctgt tggtggaaaa ttacaaaatt taccggacct
     2101 gggccccctg ggggatgggc tggatgttgt ctctggaatt ctttctgctg tatcagcaag
     2161 ctttattctg ggaaacagtg acgcacatac aggaacaaaa gctgcagcgg gtatcgaact
     2221 gacaactcag gttcttggaa atgttggtaa agctgtttcg caatatattc tggctcagag
     2281 aatggcacag gggttatcga caacagctgc aagtgcgggt ctgatcacat cggctgttat
     2341 gctggctatc agtcctcttt ctttcctggc tattgcagat aaatttgagc gagctaagca
     2401 gcttgaatca tattctgaac gatttaaaaa attgaattat gaaggggatg ctttactcgc
     2461 agcctttcat aaagaaaccg gagctataga tgcagccctg acaacaataa atactgtcct
     2521 gagttctgta tctgcgggag ttagtgccgc ctccagtgca tccctcatag gggccccgat
     2581 aagcatgctg gtgagtgcat taaccggtac gatatctggc attctggaag catcaaaaca
     2641 ggctatgttt gagcacgttg cagataaatt cgctgcccgg atcaatgaat gggaaaagga
     2701 gcatggcaaa aattattttg agaatggcta tgacgcaaga catgctgcgt ttttagaaga
     2761 ttctctgtct ttgcttgctg atttttctcg tcagcatgca gtagaaagag ctgtcgcaat
     2821 aacccagcaa cattgggatg agaagatcgg tgaacttgca ggtataaccc gtaatgctga
     2881 tcgcagtcag agtggtaagg catatattaa ttatctggag aatggaggga ttttagaggc
     2941 tcaaccgaag gagtttacac aacaagtttt tgatcctcaa aaagggacta tagacctttc
     3001 aacaggtaat gtatcaagtg ttttgacatt tgtaacgcca acatttaccc caggagaaga
     3061 agtcagagaa agaaaacaga gtggtaaata tgaatatatg acatctctta ttgtaaatgg
     3121 taaggataca tggtctgtaa aaggcataaa aaatcataaa ggtgtatatg attattcaaa
     3181 tttgattcag tttgttgaaa aggataacaa acactatcag gcgagaataa tttctgagct
     3241 cggagataaa gacgatatag tttattctgg ggcaggctca tcagaagtat ttgctggtga
     3301 aggtcatgat accgtatctt ataataagac ggatgttggt aaactaacaa ttgatgcaac
     3361 aggagcatca aaaccgggtg aatatatagt ttcaaaaaat atgtatggtg acgtgaaggt
     3421 attgcaggaa gtcgttaagg aacaggaggt gtcagtaggg aagcgaacag agaaaataca
     3481 atatcgtgat tttgaattca gaaccggtgg aattccttat gatgtgatag ataatcttca
     3541 ttctgttgaa gaactcattg gcggaaaaca tgatgatgaa ttcaaaggcg gtaagtttaa
     3601 tgatatattc catggcgcag atgggaacga ttatatcgaa ggtaattatg gtaatgatcg
     3661 actatacggc gatgatgggg atgattatat atccggagga cagggagacg accagttatt
     3721 tggtggtagt ggaaacgata aattgagtgg aggggatggt aataattatc tgacaggagg
     3781 aagcggtaat gatgagcttc aggcacacgg agcttataat attctgtcag gtggtactgg
     3841 tgatgataaa ctttatggtg gtggtggtat tgaccttctg gatggagggg aaggtaatga
     3901 ctatctgaat ggtggttttg gtaatgatat ttatgtttat aggcaaaact atggtcatca
     3961 tacaattgca gatgaaggag gtaaaggaga tcgtctgcac ttatctgata ttagctttga
     4021 tgatatcgca tttaagagag ttggaaatga tcttatcatg aataaagcca ttaatggtgt
     4081 gctttcattt aatgagtcaa atgatgtcaa tgggataaca tttaaaaact ggtttgcgaa
     4141 agatgcctca ggagtagata atcatcttgt tgaggttata acagataaag atggtcgtga
     4201 gataaaagct gataagatac ctcataataa tgaacggtca ggttatataa aagccagtaa
     4261 tatagcatct gaaaaaaaca ttgttaatat caccagtgtt gccgatgata ttaacaagat
     4321 tatttcttca gtttcagggt tcgattcagg tgatgaacga ttagcatctt tatataattt
     4381 atccttacat caaaacaaca cacactcaac aactttaacg acaactgtct gagactaaaa
     4441 gcggaggtga tggccattcc aggaggaatg gtcatcgtta tataatgagt aaatgtagtt
     4501 ctcataatag tctgtatgca ctgatattgc ttgcacaata tcataatata actgtcaatg
     4561 ctgaaactat aaggcatcag tataataccc acacacaaga ttttggggtg actgaatggt
     4621 tactggctgc gaaatctatt ggcttaaaag caaaatatgt agaaaaacat ttttccagat
     4681 tgtcaataat ttctttacct gcgttgatat ggcgggatga cggtaagcat tatatattat
     4741 ctcgtgttac taaagattca tcacgctatc ttgtttatga tccagaacag catcagtcac
     4801 taacttttag tcgggatgag ttcgaaaaac tgtatcaggg aaaagtcatt ctggttacgt
     4861 caagagcaac agtagtcgga gagttaacta aatttgattt ttcctggttt atcccctctg
     4921 ttgtgaaata caggaggatt ttacttgagg tgttaactgt ttctgctttt attcagtttc
     4981 ttgcgttaat aacacctctt ttttttcagg ttgtaatgga taaggtttta gttcaccggg
     5041 ggttttcaac gttaaatatt atcacaatag catttattat agtgatactt tttgaagtga
     5101 tattaaccgg agccagaact tatattttct ctcatactac aagtcgtatt gacgtcgaac
     5161 tgggtgctaa gttatttaga catttgcttg cattgcctgt ttcatatttt gaaaatcgca
     5221 gggtcggaga gaccgttgcc agagtaaggg aactggagca aattcgtaat tttttaaccg
     5281 gacaagcgtt gacatcagtt cttgatctgt ttttttctgt aatatttttt tgtgtcatgt
     5341 ggtattacag ccctcaatta acactggtta tattattgtc actaccttgt tatgttatat
     5401 ggtcattgtt tatatcaccc ttattacgtc gacgtcttga tgataagttt ctcaggaatg
     5461 cagaaaatca agcttttctt gtcgaaacgg taacagcaat aaatacaatt aaatccatgg
     5521 cagtatcgcc acagatgatt gctacatggg ataaacaact ggccgggtat gttgcttcca
     5581 gtttcagggt aaatctggtt gcaatgacag ggcagcaggg gatacagctg atacagaaaa
     5641 gtgtaatggt aattagtcta tggatgggag cacatcttgt catatcggga gagataagca
     5701 tcgggcagtt aatcgcgttt aatatgcttg caggtcaggt cattgctcct gttatcagac
     5761 tggctcatct ttggcaggat tttcagcagg ttggtatttc tgttgagcgc cttggggatg
     5821 tattaaatac accgatagag aaaaaatcag gcagaaatat actgcctgaa attcagggtg
     5881 atatcgaatt taaaaatgtc agatttcggt attcttctga cggtaatgtt attttgaata
     5941 atattaattt atacatatca aagggggatg ttatcggtat agttggccgt tctggctcag
     6001 gaaaaagtac attaacaaaa ctgcttcaac gcttttatat accagagacc ggacagattt
     6061 taattgatgg gcatgattta tcacttgcag atccagaatg gttacgacgc cagattggtg
     6121 ttgtattgca ggaaaatata ctattaaatc gtagtattat tgataatatt acattagctt
     6181 ctccggctgt atctatggaa caggctattg aggcagccag acttgcaggt gcccatgatt
     6241 ttattacaga actaaaagaa gggtacaata ctattgttgg agaacagggc gttggtcttt
     6301 caggagggca gcgccaacgg atcgcaatag cccgggctct tgttacaaat cctcgaattc
     6361 ttatttttga tgaagcaacc agtgctcttg attatgagtc ggaaaatata ataatgaaaa
     6421 atatgtcaag aatatgtaag aacagaaccg taattattat tgcacacagg ttgtcaactg
     6481 taaaaaatgc aaacagaata attgttatgg ataatggatt tatttctgaa gatggcacac
     6541 ataaagagct tatctccaaa aaagacagtt tatatgcata tttatatcag ttgcaggcat
     6601 aacgtatgcg cttttacatg aaaggtttat gggatttgat ttgccgttat aagacggttt
     6661 tttctgatgt ctggaaaatt cgtcacacac ttgatgctcc tgtgagagaa aaagatgagt
     6721 atgcatttct tcctgctcac cttgaactca ttgaaacacc agtatccaga cgttctcatt
     6781 ttgttatatg gagtatttta ttatttgtaa ttatatctct tctcttatct gttcttggga
     6841 aagttgaagt ggtctcagta gcaaatggta agtttactca tagtggaaga agcaaagaaa
     6901 taaaaccgat agagaatgcg attgtagaaa aaataatggt gaaagatggc gtttttgtaa
     6961 aaaaaaatga tccattagtt gaattgacgg tgcctggtgt tgaatctgat attttaaaat
     7021 cagaagcatc tttgttgtat gaaaaaacag aacaatatcg ctacgcaatc ctttctgaat
     7081 caatacaacg gaatgagctt cctgaaataa ggataacaga ttttcctggc ggagaagata
     7141 atgcaggagg tgaacatttt cagagggtta gctcattaat aaaagagcaa ttcatgacat
     7201 ggcagaacag aaaaaatcaa aagcagctca cattaaataa aaaaatagtt gaacgggatg
     7261 cagcgcttgc tcgtgttagc ctgtatgagc atcaggtatc acaggaagga agaaaactta
     7321 atgattttaa gtatttgttg aataaaaaag ctgtttctca acattcagtt atggagcagg
     7381 aaaatagcta tattcaggca aaaaatgaac atgcagtctg gcttgcacag gtttctcaac
     7441 ttgaaaaaga aatagaactt gtgcgggaag aactggcact ggagacgaat atcttcagaa
     7501 gtgaaattat cgagaagcac agaaaatcaa cagataacat tgtgttactg gagcatgagc
     7561 ttgaaaaaaa caggcagaga aaagcatcat cttttattaa agctcctgtg agtggtactg
     7621 ttcaggagtt aaatatacat acagaaggtg gtgttgtcac aacagcagaa acgctgatga
     7681 ttattgtccc tgataatgat attctcgaag taacagcatc tgtacttaac aaggatatcg
     7741 gttttataca acctggacag gaggtcgtta ttaaagtaga tgcatatcca tatacacgtc
     7801 atggttacct tacagggaaa gtaaaaaata taactgcaga ttctgtttct gttccggata
     7861 caggacttgt atttaacgtg attatatctg ttgatcggaa tgatatacag ggagaaagaa
     7921 aaaaatttcc tgttacagct ggaatgactg ttatggcaga aataaaaaca ggagtgcgta
     7981 gtgttatcag ctatcttctt agcccattaa aggaaactat taatgaaagt ttacgtgaac
     8041 gttagtatat aaataacata ttggcttttt atatggtata gatttctgaa ataatcttgt
     8101 aaatactatc gttattattt aaacaagcca tggttgaggt ttattttttt aaattttaaa
     8161 aagttaatgg gatggatatt attaaattac ttaataaagt tattatctat caacaacaga
     8221 atgagcaatc atatccagct caggagcatc tccttttaca actttgtatg cgtgttacca
     8281 aaaaacttac tgataatatt aattcatccc tgaagggggg atggtataaa tgatacaaag
     8341 cttatggttc tggccctctt atccagtgct gataattttt tcttatctcc aactgaactc
     8401 agtgaaaaat tagatatttc cagaacaaat atcaccagag tttttgattc tctagaaaaa
     8461 tttggtttta ttaggcgtat ggaaagtaag gaagatcgac gtagtaagaa tatttacctg
     8521 actccagatg gtgatttgtt attacagaag attacaagga tatatggacg ctatctaaaa
     8581 aaatatggag caaccttaca gatgataaag tcaagatatt taagtaatca gcaaaaagat
     8641 gttatctcaa aatatttctc agtaatatta catgtaatca tgtatataaa tcctgagttc
     8701 ttatttttat aatttattat tctgcgattg tgtaggggga tagatattgg ttattccccc
     8761 tcttttttct ggtaaggaaa atcagagtta cgctatgtgg attttgaacg tatttcaggt
     8821 gcataaatct gtcagaatct acatagcgca tttttcagct ttcaattgtc acttacatct
     8881 atccattgat agatcattag cgtcctggta ttaccccaaa aacgataaaa cagccctcca
     8941 cccgcttcat tactggcttc atgaaggatc tgagcaaaaa aagaatcctt ttccaatttg
     9001 gttttacccg cgataatgtg ggataagcaa acaaaaggat ctgcgcactg cgatttattg
     9061 tcgtggaagg atccgggcgt tcaggtcaga atacctgtat ccataaacag tgcgtgtgct
     9121 acgtgaaaaa taactcatga acaatgtcat tcccctgcag aattcaccag aacgcgtttc
     9181 cctgttaccc attgcgccgg gggtggattt tgcaacagcg ctctccctga gaagaatggc
     9241 cacttccacg ggggccacac cggcctacct gctggccccg gaagtgagtg cccttctctt
     9301 ctatatgccg gatcagcgtc accatatgct gttcgccacc ctctggaata ccggaatgcg
     9361 tattggcgaa gcccggatgc tgacgccgga atcatttgat ctggatggtg taagaccgtt
     9421 tgtgcggatc ctgtccgaaa aagtgcgtgt gcgacgcgga cgcccgccaa aagatgaggt
     9481 ccgcctggtt ccgctgacag atataagcta tgtcaggcag atggaaagct ggatgatcac
     9541 cacccggccc cgtcgttgtg aaccattatg ggccgtgacc gacgaaacca tgcgcaactg
     9601 gctgaagcag gctgtcaggc gggccgaagc tgacggagta cacttttcga ttccggtcac
     9661 accacacacc ttccggcaca gctatatcat gcacatgctt tatcaccgcc agccccggaa
     9721 agtcattcag gcactggctg gtcacaggga tccacgttct atggaggttt ataccagagt
     9781 ttttgcgctg gatatggctg ccacgctggc agtgcctttc acaggtgacg ggcgcgatgc
     9841 tgcagagatc ctgcgtacac tgcctcccct gaagtgacag catacgctct tccttatata
     9901 agcggaggta agcagaacag gcataacata agctatagca ggtagataaa ctgtagtgag
     9961 taagccgtgt tttatgtaag gaacaggcag atggcagctc agccacagac agtgcagcag
    10021 gaaactgaat ataaactaca gtgagcacat cactcactgc acctgttaat ataaactgta
    10081 gtcagtaaag agtctctctt atctgactac agtttatgtt cagcgggatt tgaagagttt
    10141 ttccaggtca tccagcgtga tgcccagttt ttcaagcagg gcaagcttct ccgccatctc
    10201 cggactgatt ttttccgcag gtgaaggtgg caactgattt tccttactct catcattcgg
    10261 cgcttttaac cggggacgcc ggtagtgaat acagaagaat tttgtccgcc cccgctggat
    10321 ctccgtgtaa tcaagatatc caatctcgcg caactgctcc attgcccgtc tgaccgtctg
    10381 gttctgggaa aatacaggag acttcagatt gaggcgtgca cgcagccgcg ccagcgatat
    10441 cggtgccgga tcccggggca ggctctctat aaaggtgtaa agtgcttggg cggactcccg
    10501 tcgcttcagg gcattaatcg ccttaagctg aagaaggacc tttctgtcaa actggtacag
    10561 ttcaaaaagg cggggatcag cctgtaactg aacaatatcc cgttcagtat cgtagtaggc
    10621 tgactgtacc agatgggtga tgtattcccg agtgtgcttc tcatcggtgc gggaaaacga
    10681 gatcacggta ccggcaatgc gcttcaggga agggctgatg cgctcacgca gcctgcggga
    10741 tgactggctt gaaggtatac cacacagttt tgcaaactca acaaaaggca gttcaacttt
    10801 gtcaccaatt acgttatggc gggcaaagga atgaatgatc cccacccagg tcttgaaatc
    10861 gttatccata tccagccggg ggccggtgat ctcaacctta tcgaatcctt ctgcacgggc
    10921 cagagaaaga cgcgtcagtt cttccgtggc atcggtgcgt gacagtgtat tttttttact
    10981 gttcttcagt gacttaaggg tcggtacaaa gacgcccaga cgcatcagcg ccacaggttg
    11041 tacggtgttg ttattgtttg gtgtcagtgt caccagctca ccggacgact tatccacctc
    11101 gccgaacagt tttttgatgt ctgaattttc gttttccaga aagcctcctg cctgtggaca
    11161 gtaaaagaat aaacagcaaa tcgtgtgtca gagccccgct aactgactga agaaaaacct
    11221 gcagcctgta tttaacaact gagtctggcg tactgaacat agtttatgtc attaacttac
    11281 tccagtatat ggattactga atacagctta tcattagcat gttacagttt atcagtaaca
    11341 tgccacagct tatgtgatcg tggtcccagc ccatgagtga caaggtttgc agagatatgg
    11401 ggatctttaa tggatccttt gttgatcatt catggatctg tttactggat cgaacctctg
    11461 gatatgtgga taaatcatcc caggggaaac agcatacaca accgcttata agggcttcag
    11521 ggcgtacctg ctcactgtaa ctacagatac attgcgcttc ctcgccggtt cgcgggcttc
    11581 gcccggtcac cggtctgcgg cgagcactgt tttttctgaa gtctgtcttt acaggtaatt
    11641 tttgacggaa tctgcagtac ttcctgagcg tacggtcata tccgaagtac acttcatatg
    11701 tgtgtttcaa tgccagggat tgtgtataca aactacaggc aaagtcaggt tatcaccggt
    11761 ggtaggctgc tccgatggaa aaattgtgaa ctgattgaca ccatgcctga taactctgtc
    11821 gtgttgtcag cgccagtgat ataagacggt caaacatcgt ggcgttgaca catacagaga
    11881 gccggatgct gtttgctgat gctgattctc ttcgtatatc tccccgcgag gcaaggtcgt
    11941 taattgaaca ggctgaaaaa cggcagaagg atgcgcagaa cgcagacaag aaggccgctg
    12001 atatgcttgc tgaatacgag cgcagaaaag gtattctgaa tacgcggttg tcagagctgg
    12061 aaaaaaaggg cggggcagcc cttgccgttc ttgatgcaca acaggcccgt ctgctcgagc
    12121 agcagacacg gaatgacagg gccatttcag aggcccggaa taaactcagt tcggtgacgg
    12181 aatcgcttaa cacggcccgt aatgcattaa ccagagctga acaacagctg atgtaacaga
    12241 aaaacacgcc tgacggcaaa acgatagttt cccctgaaaa attcccgggg cgttcatcaa
    12301 caaatcattc tattgttgtg agcggtgatc cgagatttgc cggtgtgata aaaatcacaa
    12361 ccagcgcagt catcgataac cgtgcaaacc tgaattatct tctgactcat tccggtctgg
    12421 attataaacg caatattctg aatgaccgga atccggtggt gacagaggat gtggaaggtg
    12481 acaagaaaat ttataatgct gaagttgctg aatgggataa gttacggcaa cgattgcttg
    12541 atgccagaaa taaaatcacc tctgctgaat ctgcggtaaa ttcggcgaga aataacctca
    12601 gtgccagaac aaatgagcaa aagcatgcaa atgacgctct taatgccctg ttgaaggaaa
    12661 aagagaatat ccgtaaccag cttgccggca tcaatcagaa gatagcggaa gagaaaagaa
    12721 aacaggatga actgaaggca acgaaagacg caattaattt cacaacagag ttcctgaaat
    12781 cagtttcaga aaaatatggt gcaaaagctg agcagttagc cagagagatg gccgggcagg
    12841 ctaaagggaa gaaaatacgt aatgttgaag aggcattaaa aacgtatgaa aagtaccggg
    12901 ctgacattaa caaaaaaatt aatgcaaaag atcgtgcagc gattgccgca gcccttgagt
    12961 ctgtgaagct gtctgatata tcgtctaatc tgaacagatt cagtcgggga ctgggatatg
    13021 caggaaaatt tacaagtctt gctgactgga tcactgagtt tggtaaggct gtccggacag
    13081 agaactggcg tcctcttttt gttaaaacag aagccattat agcaggcaat gccgcaacgg
    13141 ctcttgttgc actggtcttc agtattctta ccggaagcgc tttaggcatt atcgggtatg
    13201 gtttactgat ggctgtcacc ggtgcgctga ttgatgaatc gcttgtggaa aaagcgaata
    13261 agttctgggg tatttaaata aaatataaga caggctgtct atcttacaga cagccttttt
    13321 ataataacag aaagaatata tatcatactt aacggaagag ataatatgaa aacagtaccc
    13381 gtataaacag caagtaatcc catttttcca ggtgtatcag caaaaaaacc gctgttccag
    13441 aaatcaggcc gggtaaattt cagagctgta tcctcaatat accattttgc aaccggatac
    13501 aaaaccattc cgcagagaga aatacaccag aacagtaatc tgtatttaaa atcataatcc
    13561 catgacatat acagcatata tccccccgtc acccatcccc accacatatt attaaaataa
    13621 tattttctgt tcatctgcct gttttccttt cttttgcact tttgtcagtg tactgatgca
    13681 tgacaacata accacagcat gataataata atcaataaca ataagctgtg tcacgtttac
    13741 atcatggaac ggaggtgctc ctgtgacttt tgagcagaaa aaagcccggg ccattgccct
    13801 gatggacagc aaaaagatgt ggcggagcaa ttagcccctc ctcttctgcg tattctctgg
    13861 cggctgggta tccggcttcc gccattaccc tttatgccgt tctggcaggt cacgctgttg
    13921 atggggagct tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg
    13981 tcaggaatgg tggcaggtga ggccattatt ataagtatca cggggggttt ttggttcgga
    14041 ctgctcatgg cctctttcca ctggtggcgc aggaaagtta accggctgcc accatgggat
    14101 gatgtgtaaa ccgtgcacag gcgtgttctt acgaagcggc actttcattt atctgtacgc
    14161 gaacctctat tcctgcgtca tgacaggcct gcagccactg cgccacttcc agcggatcgc
    14221 cctcccggcg taccaccctg ccttctttat tccataactg cagacaggtg ctgccgtcga
    14281 gacgcaccac aaaatcccca cggcaggcct gatacgttat gccgttcagc tccaccgcca
    14341 gatcagtggc agaagtgccg tctgcgggca gacctgctgc cgggaccagc ggcagtacga
    14401 cgacttctgc cgcacgctgt tctgcccgga ggcggtcctg ttctgccagc agcagaggct
    14461 gtgccacggc ccggccggcg tccgtcagct cgacagccag ctgcagattc ggggcgcgca
    14521 gggtacgaag ccagccggca tcctccagac gacggcagga ggcgcgcagg tttggcccgt
    14581 agaccggggc ctccccgctt cgttccagca cccgttccag gtcccgcgtc agcacgggtc
    14641 ccggccgttt ttcatcaagg gcagccagca caatcagcac tctccgctgc agtggcgacg
    14701 gtcggcggct ctgggtcatt acctgtcatc ctccggccag atgaggtttc tgaggcgctg
    14761 aaagtcggcg tcaatgagct cctgtctctt tatggcattc attgaggcat agccatttat
    14821 ggccagttca tgccagaaga tcagcacggc tccggctttg gctgactcgc agagataaat
    14881 agcgccctgg ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc gggtcagttg
    14941 ttcatagctg agtgaaaacg tcatttcctg atccatcctg cacgctccat ctgataatgt
    15001 tctgacaggt aaaaataata tcctgcatga tttgtgtgat cctgatttct gataatatca
    15061 gatatggttt tatcagcaat gatgaaaatc atatcagata tgattttatg tgtgtttttt
    15121 attatcatat tatttgcgtc ttttctgccg tgcagatccg gtttccggcg gttacggatc
    15181 ttcctccccc tccgcacagc gccacaccct gtaagcacaa catgtagtgc tgaattcagc
    15241 tgctcttgac actatatgtt gtgtcatctc cctgacctgt gatgcgccgc gcaggggcgg
    15301 aaaacagcga tatgattatt tccccagcgt ggtacacttc cggaaagttg tgatattccg
    15361 gaaagtcgga tctgacggaa acggctctcc ggtaatttaa cggcgtggtt atatggatgc
    15421 ttgttatcat ggtgatgatg ataacggcat gatgttatca gacggcgtga cggtaagggc
    15481 agtgatgatg gatgacgtta tcgcatgacc gtccctgccc ggaaaagaaa aaaggagtca
    15541 cccatgtttt ttattgagaa tgaaggtcag gctgtcgcca gaacggatta ctggcagtct
    15601 gtacaggcgc aggccggata tgtctacctc agctggaatg ccggcgcagc caggctgctt
    15661 gtcccggatg cggcaaaaca tttactcagg gagatgcggg gggctgagta cgtcatcatc
    15721 agtaagggaa cgctgaatgg ccgcgatgcg ctggaactgg tatttgaaga cggcagcgat
    15781 gcgccgtttg tgatccacat gctgagtgag cagtgcgatc gcctgctccc cgaaaacaac
    15841 cagggagggg gttttgttgt caccgtccgg acgcgtggcg gtaaccagct ccgttatccg
    15901 ggaaagtacc gtgttgtgga aaacctgccc gacgtttccc cgtggagtga acactgatat
    15961 gcagcacctg ccggcaccga tccaccatgc ccgggatgcc gctcagcttc ctgttgccat
    16021 cgattatccg gcagcgctgg cactccgcca gatgtcgatg gttcatgatg aactgccaaa
    16081 atacctgctg gcccctgaag tgagcgccct gctccattac gtcccggatc tgcgccgcaa
    16141 aatgctgctg gccacactgt ggaacaccgg tgcgcgcatt aatgaagcac tggcgctgac
    16201 gcggggggat ttttcgcttg cgcctccgta tccgtttgtg cagcttgcga ccctgaaaca
    16261 acggaccgaa aaagccgcca ggacggcagg aagaatgccc gccggtcagc agactcaccg
    16321 gctggttccg ctctccgacg cctggtacgt cagccagctg cagacgatgg tggccacact
    16381 gaaaatcccc atggaacggc gtaataaacg aacaggcagg acagagaaag cgcggatctg
    16441 ggaagtgacg gacagaacgg tcaggacctg gataggggag gcggttgccg ccgctgccgc
    16501 tgacggtgtg acgttctctg tcccggtcac gccacatacg ttccgccatt cctatgcgat
    16561 gcatatgctg tatgccggta taccgctgaa ggtcctgcag agtctgatgg ggcataagtc
    16621 catcagctca acggaggtct acacgaaggt gtttgcactg gatgtggctg cacggcaccg
    16681 ggtgcagttt tcgatgcctg agtccgatgc ggtgtctatg ctcaaaagaa ttccataagc
    16741 tctgactttt ttaaatccct ttcggatatc tcatggatat attatcggta tttcatattc
    16801 tgttgacatg caggaccggt atagagtaaa aaaacatcta ttgagtacct cttggatatc
    16861 cgaaaggtgt cttatggggt ctttatggat attacatgtg gattctggag atacactatg
    16921 cctgttattg ctaatacgca tcccaaaggt ggtgtgggta agacaacttc atccgtaaac
    16981 attgttggtg aaatgaagtc tgatacagtt gatctggata ctcataccgg gctttctatc
    17041 attctggggc tgagacctga aggaaaagaa atttctgtga aagtacccaa aacagtggat
    17101 gagttaatcg aaattatgac tccctacaag aacagcgata agacgttact tattgactgt
    17161 gggggatttg actcagatct tacccgtact gcgattgcct ttgcagactg cgttattgtc
    17221 ccttcaaaag actccctgac tgaacgtatt ggtttgatgc attttgatgg agtactggac
    17281 gaaatcagct ctatcatggg gaccgatata actgctcacc tgtatctctg taaagttaat
    17341 cctaacaaga agaaattccc taagcttgat gcaatcctgc catcgttcaa acatctgaag
    17401 ctgatgaaga gccgcatttc tgctcgtgca gaatttgagg atgtcattga gactgggatg
    17461 ggaatcactg agtctgttca tgggcgctat tcggcaggag gtaaggaagt tattgccctg
    17521 atcgaagaaa tcaatcatct gattgaaaat aacaaacagt aaggtgtgtc tattgggtat
    17581 ccatatagta tcctttgggc ttttaatgga tatcttttaa gtatctaaca aacatacttg
    17641 gttatctcat gagcaaagtg aaatttagtg cacaggctga aaaactgaaa aacctggtgg
    17701 attctgccag attacaaact tcttcttctg ccgtaacagc aacagccccg tctcagccaa
    17761 atgaaggtgc agaggtagtt tcaggccgtc ccgtggctgc caggacgaag gctaagaatc
    17821 ttaaggccat accgctgtcg tattttgaag ctcatgcgga acttaaagcg accagtaaaa
    17881 catctctgga ttttagctca tatattattg aggctataag agagaagctt gaacgtgatg
    17941 gtgcaatagg tcaataaact atcggatacc ttaagggtat ctgatgtgtg tcttttaaat
    18001 gcgttatgga gattcttcac gtacctaaag agtccccatt agacatcttt ctgtttatgg
    18061 tgacatgatt cgtcaccgct ttcctttcct ctcagtttca ccatgtcatt ccttttccct
    18121 taacctgaac catgatgtca aagatgtttt tatattgcat ttatgtatta catataacct
    18181 tcataatgtt tttgtatcta tgatttcctc tggtcttaaa gttgtgtgta caatgcacat
    18241 gcaatttatc gtgtttttat gttgcatttc tcctttcaga aagaccattt tttcattata
    18301 attaaatata cattgtatgt atgaagcatg tttgtttttt ctatttgttt atgagcaacg
    18361 gagactggtt atgaaaatct ttattgatga tggttctacc aacattaaac ttgcctggtt
    18421 ggaggatggt gacgtgaaaa cgttaatcag ccctaacagc tttaaaccgg aatggtcttt
    18481 tagtttactg gatgatgctg ccccggctaa ttacgagatc gacggcgaga agttttcatt
    18541 tgatccatta agtgctgatg ctgtcgtaac gactgaaaca cgctatcagt acagtgatgt
    18601 gaatgttgtg gccattcagc atgcgctaca gcagaccggc ctgaaagcgc agcctgttga
    18661 tgtcatcgtc acattgccga tctctgaata ccttgacgcc aacaatcaga aaaataagca
    18721 aaacattgaa agaaagaaaa aaaatgtgat gcgtgaagta cgggtgcagg ggagtgatgc
    18781 atttgttatt cgcagtgttt ccgttcttcc tgaaagtatt cccgctggtt tcagtgttct
    18841 tgcggggctt gaggatgatg aatccctttt gatcgtagat ctcggtggta caactcttga
    18901 tgtgtcgcat gttcgtagca aaatgacagg gattacaaaa acctggtgtg atccgaatat
    18961 cggcgtgtcc ctgattacat ccggggttaa ggaacagatg gctgtacatg ctaatacaag
    19021 agtgagctca ttccaggctg acaatatcat tgtgcaccgt aatgaacctg actacctttc
    19081 ccgtcgcatt tataatgctg aacagcgtga gtctatcatt aatgttatca atgaacggca
    19141 gaagttgctt attaaacgag ttaatgatgt catcagccgt ttcaccgact atacacatgt
    19201 aatgtgtgtt ggtggtgggg ctgaaattgt ggccgaggct gtgaagaacc tgactaaggt
    19261 tcctgatgag cgcttttatc tgagctcatc cccgcagttt gatttagtca tgggaatgat
    19321 aaaaatgaaa ggtggtgtga ctaatgagtg atgaaaataa atccagacgc tgctcatttg
    19381 aacttttccc ggatgaacgg acaggagata agatagcaga tgagctcatt gcaaatgaaa
    19441 aactgaagga gcggggacgt ttcatgcgcg cgatgttagt tactggtgcg gcgtttgcag
    19501 ctatagataa acgtcttcct ttgctgatat cggagttact gacagaaaat acgactctgg
    19561 atgatattaa taaggtgatt tccagcgtga taccaggtgc tttttcggtt gaaaagaaat
    19621 tgcttgaatt actggaaaaa caatctggtc tccatacgtc tgtggattgc agtactcctc
    19681 ttacagagca gagtctgtcg agaaatgacg gtgaagacca gaccagaaga aacgctgaaa
    19741 atatgttcgg agatgactga actaacccag cctggccagg ggaatatctc gccatttagt
    19801 tgtataacac tgactcaaca tttcccgttt catttgccat tcaggggcgg ttcctcgccc
    19861 cgcaaaccac actttccctt ttcccgactg gttcagttca tcgagtgttt tcattaactt
    19921 ttcactgttt ttacggggct ggatttcatc aaataatccc ggctgcgcta tacccgatgg
    19981 tgtgaaatca gccagcatga ctcctgcctt catatagcgg tacccttcac gccagacatg
    20041 gtttaaggct ctgcatgcgg cggcaataat gtcccggctg tcctgtgtgg gcaatggaag
    20101 cttttccaca gcggcattgc tgtaacaggg ttcttttact gcaaaggggg atgtccgtac
    20161 aaatgtcgtc acctgccggc aatactgacg ctccccacgt agtttctctg cggcccgctc
    20221 tgcatactga acaacagcct ggtgcatggc atctttgtct gtgattcgtt caccaaaact
    20281 gcgactacag acaatctgct gttttgccgg tggtgcttct tccagggata tgcaggactc
    20341 gccgttgagt tcgcgtaccg tacgctcaag aatgacgctg aagtttttcc ggatgaatgc
    20401 cgtgttagcc tgcgccagct gcagtgctgt gttaataccc agcgcattca gcttttccgt
    20461 cagtctgcgt cctactcccc agatctcacc aactggctgc agtcccagta gcttcaagat
    20521 ccgattacgg ttttctgccg tcagcgcgac cacaccggaa aactgtggcc attgctttgt
    20581 tgcccactgt gcacttttag ccagcgtttt tgtaggcgca atgcccaccc ccatggtgag
    20641 tcctgtccag ctctttacct gttccctgag ctgatgacca aaaaactccg gagagatgca
    20701 atgatttatc ccccgcaaat caataaacat ttcatcaatt gagtagggct caactgcggg
    20761 agaaagcgac tccagaacag ccatgacccg ttggctcatg ctgtggtaca gcgcataatt
    20821 gctggaaaat acatgtattt tcttctccag gcgcatttgt ctcacctgaa accagggctg
    20881 ccccattctg atgccaaggg cttttgcctc cgggctgcgc gcgatcacac agccatcgtt
    20941 attgctgagt acgatgaccg gttcgttgcg aaggtccggg cggaaaactt tttcacatga
    21001 ggcgtagaaa ctgttgatat cagccagtgc aaacatcagc gtaactcccg cgtcctgtgt
    21061 atcacgtgag tgacaacacc aaaaatacag atgttttccg gatacagtgt gcggaattcc
    21121 gggctgtctg aaaccggctc cagtgccggg cgtgggcgca acagcagtcg tttgacggtg
    21181 aactcaccgt cgatctcagc gataacgatg tccccgtgtt gtggtttttc ggccctgtcc
    21241 actaccagca gatcaccatt ctgcacgcca gcctggttca tcgattcacc gctggcgcgc
    21301 agaaagaagg tggctgcagg tctgctgatg caatagctgt tcagatccag ttcctgctca
    21361 gcataatcag tggcaggcga aggaaaaccg gcctggcaac gatcggcaaa caacggtcgc
    21421 acgtaactgt catcgcctga agggtcagcc ggacggtgat aaacggtact catgctgcag
    21481 gcagccagct gtcgtcctgc cagatttctt ccagcagaga catgatacgt tctttatcct
    21541 tgtcgttgcg ggctcctgtg acagatacag acggggcgct gccctggtct atacgcagcc
    21601 agatttccgg gtagtgtggt ttcagacgtt gttcgatttc attctgtagc gcctgaagtg
    21661 tgcctgattt gaggtttttt gtgctctggc ggtcgaaaag aatttcaatg cggatcatgt
    21721 tctcatctcc cttttctatt gctgtatgta tatacagcat tctaataact gaatccacac
    21781 agtcaaatgt cattattgtg actaaaaaac gctcaattat ctgagggggt ggctgtatgc
    21841 ctgctgtgga ctgctgtgtc cagcttatcc acagcatttt gcgcacggtt ctgtgaataa
    21901 aatacctggt tacccaggcc gtgccggcac gttaaccggg ctgcatccga tgcaagtgtg
    21961 tcgctgtcgc cggcctcctc acccggtcac gtttcgtcgt tcctcctcca cgcgctgcgg
    22021 cttcggggcc gcacctgcat tcgtatgcgg tcgcccggtt acaggtgcgg cacggcctga
    22081 tggaggccgc atgtgagagg agaattccca tgccaaactg gtgctcaaat cgtatgcatt
    22141 tttctggtga accggcacag attgctgaga ttaaacgact ggccagcggt gcagtcacac
    22201 cgctttatcg ccgcgccaca aatgaaggta ttcagctgtt tctggccgga agtgccggac
    22261 ttctgcagac cactgaagat gtgcggtttg aaccatgccc cggactgacg gctgccggac
    22321 gtggcgttgt atcgccggag aatatcgcgt tcacccgctg gctgacacac ctgcaggacg
    22381 gtgtactgct ggatgagcaa aactgcctga tgctgcatga actctggctg cagagcggta
    22441 ctggacagcg tcgctggaaa ggattaccgg acgatgtcag ggataccatc accgcacttt
    22501 tcaccgcaaa aagaggtgac tggtgtggct tctggagtaa cgaggatgta tcggtgtggt
    22561 ggaaccgtct gtgtgacaac gtactgccgg aaaaaaccat gccgtttgac ctgctgacgg
    22621 ttctgccgac ccgcctggat gttgaagtga atggctttaa cggtggtgtt ctgaacggtg
    22681 ttccttctgc atatcactgg tatacggaac ggtatggcgt gaagtggcct gtggggtatg
    22741 aggtgaatat cagtagtcag ggagacaact tcattcaggt ggatttcgac acgccgtggt
    22801 gtcagccgga aagcgacgtt attgcagaat taagccgccg tttcagctgc acgctggagc
    22861 actggtatgc cgaacagggc tgtgatttct gtggctggca gttgtatgag cgcggagagc
    22921 tcgttgatgt gctgtggggg gaactggaat ggtcttcccc gacagatgac gatgagctgc
    22981 cggaagtcac cggacctgcg tggatagtcg acaatgtggc gcattatggc ggatgaagta
    23041 tgacggagac gggcgggcaa ccgcccgttt cttttccggt aaaggatgtc gctgtgctcc
    23101 tctttttact gcgccggctg acgcggcgcg gcataaacgc tgcctgtggt cagtgtcccg
    23161 cgtccggcgg gcacgggacg ggcgctttta ccgctcccgg ctggcgtcgg tgacagtgta
    23221 cgccagcctg tcgccctttt ctgatgaacg cccttcaagc cgctttcgcg gcataacctt
    23281 gccgtcaaaa agacgacggc aaggtattcc acggtcggcc tgccccgtta ccggacgcgg
    23341 tgaacagccc acaggcagcg gggaacgggc accacagggg ggccctcccg gagccctctg
    23401 taagagaagg agttttgtat gtcccgtttt gtccttggta actgcattga tgttatggcc
    23461 cgtattcctg ataacgccat tgatttcatc cttaccgatc ctccatatct cgtcggtttc
    23521 cgtgaccgtc aggggcgcac cattgctggt gacgttaatg atgactggct gcaacctgcc
    23581 agcaatgaaa tgtaccgcgt actcaaaaaa gatgcgctga tggtgagttt ctatggctgg
    23641 aaccgtattg accgctttat ggcagcctgg aaacgcgccg gattcagcgt ggcagggcac
    23701 cttgtgttca ccaaaaacta cacatccaaa tctgcgtatg tcgcgtaccg ccacgaatgc
    23761 gcttacatcc tggcaaaggg cagtccggcc cggccccgta acccgctgcc ggatgtgctg
    23821 ggctggaaat attcgggcaa ccgccatcac ccgacggaaa agcccgttac cagcctgcaa
    23881 ccactgattg agagcttcac acatccgaac gcgattgtgc tggatccgtt tgcaggcagc
    23941 ggctcaacct gcgtcgccgc cctccagtcc ggacgccggt atatcggtat cgagctgctt
    24001 gagcagtatc accgtgccgg acagcaacgt ctggctgccg tacagcgggc catgcagcag
    24061 ggggccgcaa atgatgactg gtttatgccg gaggctgcgt aaatgaacta tgccggacat
    24121 gaaaaacttc gcgccgaagt ggcggaggtg gccaatgcca tgtgcgacct gcgtacaacc
    24181 atgaatgaga tggagcggcg gtacagcttt aatgctgaca ccctgccgga acgtctggtg
    24241 cgtcagacgt tgtttcgcgc aaaccgcctc ctgatggagg catataccga aattctggaa
    24301 ctggattcct gcttcaaaga ttgaggggag agagtatgta cggaacatgc gaaatactct
    24361 gccgggagct ggcagcaaag tatccgggag acacgccgct gatgctggtt atctggtctc
    24421 cggaagagat tcaggccctt gctgacggaa tggatatttc cctgtccgat catgaaatca
    24481 ggaccgtcct ggcgcgcctg gaggacatcc cggaagacca gcggactgag tccggtattt
    24541 cttcgggtgc ggctatggag attatcagca acgtgagtga aaaccgccag gtgaccgtcc
    24601 ctgctgaact gctggcgtcc ctgattcaga ccgctgaaca ggcattgtgg aagcgtgaat
    24661 gggccgcccg tgataacggc ctcgtcgtcc cggaatgcgt cacccgccgt caggcggtgg
    24721 ttaatcaggc ccgcgccctg ctgaaaaaca acacacacga aaacgactga tgttatcgcc
    24781 gctttcgggc ggcgattcag ggagaccatt aatcatgaac aaaacgctga atgcactggt
    24841 ttgtcgccac gcccgtaacc tgctgctggc gcagggctgg ccggaagaga cggatattga
    24901 acaactgaat ccgcactatc cgggctggat cagcatttat gtgcgactgg atgtatcccg
    24961 gctggcgacg ttacttatca gccgccatga tggcgtgctg ccgccggtcc tggcgtctgc
    25021 catgcaaaaa ctgaccggaa ccgtggcgga acttgtactg tccggcagcc agtggcaggc
    25081 gctgccggta cttccggcag acggaacgca ggtgtctttc ccgtatgccg gtgaatggct
    25141 gacggaggaa gaaatccggg cagttcttga tgcggtgcgc aatgcagtac gaagcatctg
    25201 ttaccaggtg gcagacgatg cgcggcgtat ccgggcggcg ctgaccacca ccggtcagac
    25261 gttgctgacc cgccagacgc gccgctttcg tctggttgtg aaggaaagcg attacccctg
    25321 ctggcttgat gaagatgacg aaaatctgcc cgtggtgctc gatgccattc tgaaccgtgg
    25381 cgcccgtttt tcctctgtgg aaatgtacct ggtctgtgag tgtgtggagc atatcctggc
    25441 cagtggtctg gtctgtgatg tgctgcgtat accggatgaa ccgtcccgtc gctggtttga
    25501 cagggatatt ctgcgggagg ttgttctgga ggcacgggat gaaatccgta gcatggcgga
    25561 tgcactggcg aaaatccgga aatgatgacg ctggcgggga aaaatctccg ccttttcctg
    25621 ccggtccccg gaaagagcaa aaggatgtcg ccgcaggctc cgctttaccc ggccatgcag
    25681 ggcatggcct tgtgggtttt cagttatgtg gccgccgtgt cgtgtgcggg ctgtgccgtg
    25741 cctccatctt agccgggctg gcggggatgc aagggtaaaa tgcacgcggc ggtcacccgg
    25801 tccctccttc ccgtctgccg tgattttccg gtcgttcacc cgctcagatt tcgcggtctc
    25861 accctccaac tcccgccagc cgtgtgcgtc aggctgcggc ttcccttgcg cccttgcatc
    25921 cccgccttat cgcccggctt ttctggaggc acggcaccgc ccggtgtcgc ggaatatgtg
    25981 aacaacggag gaaatcccat gcagtatgcg aaacctgtca ctctgaacgt tgaagagtgc
    26041 gaccgccttt cctttctgcc ttacctgttt ggcaacgatt ttctgtatgc cgaggcgtat
    26101 gtgtacgcgc tggcgcaaaa aatgatgccg gaatatcagg gaggcttctg gcatttcatc
    26161 cgcctgccgg atggtggcgg ttacatgatg ccggatggcg accgttttca tctggtcaac
    26221 ggtgaaaact ggtttgaccg cactgtgagt gctgatgccg caggtatcat cctgacttcc
    26281 cttgtgatta accgccagtt gtggctgtac cacgacagcg gggatgcggg actgacacac
    26341 ctttaccgga tgcgtgatgc gcagttgtgg agccacattg aatttcatcc cgaatgtaac
    26401 gcgatttacg cagcactgga ctgattaacg gacggggcgg cgatgccgcc cctgtaaagg
    26461 agacgagaac atgtactgta ctgttaaaga aattatccgc gaagtgctga atacagacgt
    26521 gccggacagt gaatgtgttt ttgccgtggt gctgacccgc ggggatgtgc gccacatagc
    26581 ccgggactgg agtctgacag acgatgagct ggaaaccgtc atgcagcggc tggacgatgc
    26641 ttttgtatat ggcgcatgtg acagagttgt ttccgatatt gtcaatgaac tgatggagga
    26701 aaagcgcgta aaccgtcttg taacagtccc tgcggtgctg ctggaaaaag tgatggtgat
    26761 ggctggtagt gaaatttatc gcctccatgc tgtcgggagc gagaacgggg gcgacggtga
    26821 cgcatttgtt agggaagaac gtgagattat gcgggtaatg cgtcaggcgc tggacgggga
    26881 gaacgggtaa tgaatatcag cacggaaacc cgcgaaattc tgcgcaatta caaagccgtg
    26941 attaatgcgc ggcgtcgtga aatggggcag aaaccgctca ccaccgcgca gattgttgat
    27001 gaaatctgtg attttgtggc gaatcagcag gcggttttcc tcggtggtca ctatatcctt
    27061 cagggcagca gaaacaggtg atacagggca ggcggtggag gccgcctgtc agcccgcgcg
    27121 aaaacccctg gcaggctggc gcctgccgga agggaagcgg gccacgctgg cgcgtcgccc
    27181 gttacccttc ccgtttgtga tgtgtccgga atgttttttc ctgatgttat gtttctgtgg
    27241 ttactgttct tcttcttcct cttctttccg ttcctctcct gtttatggct ttcccgggcg
    27301 tccccctggc ggggcgggct gtcgtgaacg gagccagcgg agctgtctcc gccctggcgg
    27361 gcttccatcc ctgacgcaaa aaaccgggtt tgcagcgacc ggaacggtcg cgccaccctt
    27421 gtcgcgccgt ggcctgcgct ccttacatcc cggcaaaagg ccgccgggat gtaaggagca
    27481 acgccgtttc gcaaaccgcg cttcacgtcg ttgcagcggg tccctgctca ttgcctgccg
    27541 ggtgccacac acccgtatga gtccctgtcg ccgggggcaa agacagccac aaccgtctcc
    27601 gccagtccag gggtgaaatg tacatcccac aaaacttttt gcccaaaagc aggcaaaaac
    27661 ttttgaggtc tgcccccgga cagtcgtttc cggccgtggc agtgatgccc cttgcggcga
    27721 aggttctcaa acgggtgaag ttcataccac agggaataca gcagcaggtt cccgcgaaac
    27781 gtcaaaaccg cggctcccga aactcaacgg gtgatgttct ttaaaaatca ggatttattt
    27841 aaggctccgg caatacgcat ttattcgctc tgtcagcatc cggctggcag gtgatgagaa
    27901 cgcaggaaca gaaggaggaa tacaccggaa aacgtcagat gcagatatga aaaaagcagg
    27961 tcagggacct gcttaatcca cgccgaagag actgtgcaac cagtctttaa ggcactgtta
    28021 ccaacctgtc ataaggaatc catttatgac tgtaagcatt gtttcacctt ccgcagcggc
    28081 tgtaaagccc cgtcgtcatc cccgtttcag acgcgaagac atccccgcgc cggaaattga
    28141 ccccgtgctg aaagccttcg ggcgacacat tgcccggagt tttcgccggg ggcgtggtgt
    28201 acacatcccc gccatgaaaa acacggcctt cgggcaggtg ctccgcacac tcgaactgaa
    28261 acgcgctttt aactgatgag gtgagccgcc ccgcgagggg cggcaacaga cagaaggaga
    28321 acattttatg acattatcag caatcaccat gaccgccccg gaagccgcca gcccggtgca
    28381 gatgtaccgc gcgacctact caccggatga taacaaactt cgcctgtatg ccgcgtcacg
    28441 cctcgacccg gagacgtata aaaaagtgca tgatgccggt ttccgctggg caccagaaca
    28501 ggcgctgttt gtcgcgccag cctggacacc gggccgggaa gacgtgctcc tctcacttgc
    28561 cggagagatt gaggatgaag acagcacgct cgctgaacgt caggaagcac gggcggagcg
    28621 gtttaccgga tacagcggaa agcgggccag tgaatccgca caggcacttg atgaagtgga
    28681 aagactggcc gcgatgatcc cgcccggtca gcccattctt gtggggcatc acagcgaacg
    28741 ccgcgcccgt cgtgatgcgc agcgtattga aaacggcatg aaacgtgccg tgatgctctt
    28801 tgaacgtgcg gaatactggg aagaacgggc gcggtcggca ctgcttcacg cgaagtataa
    28861 agaacgtccg gatgttcgct ggcgtcgtat caaaaaaatc gaagctgatt tgcgcaaggc
    28921 tgaaaagacc atcgcaaagt cgcagaaata tctgacgatg tggcgggctg aatcgctgga
    28981 tctgaatatg gcaaaactca tcagcagtca tgaccatatc agcgcctgtt tcccgctgga
    29041 tacgtatccg cgcccggcag aaaaaagcca gtatgaagga agtcgatcgt tatggtcggc
    29101 cctggatgat gacatcatca ccacggagca ggcccgcgaa attgcgatcc gctgccatga
    29161 acggcagatc cagcatcagc aacggtgggt taaccactat cagaaccgcc tgatttatga
    29221 gcgtgccatg ctggacgaaa gcggcggcgt ggttacccgg acacaggatt ttgaaccggg
    29281 cgggcaggtt ttcagccggg gcgagtggct gaccatcatc cgcgtgaaca aaagcaacgg
    29341 ggcggtgagt tcagtcacaa cggcgaatta cagttttctc gggtacagcg gcacgatgaa
    29401 agtgacgccc gatcgcatca cggactacaa agcaccatcg gcagaagatg ccgccgtcgc
    29461 cagacaggcc gcgaagcgtc caccggttgt caactatccg ggagaaggtt tccgggaaat
    29521 gacaaaggca cagtgggccg ccctgccccg ggactgtaag gccgtgcgca gtgtggcaga
    29581 agcagaagac cacggggcat accgctaccg ccgcacaatg gacaataatt tccgtctggt
    29641 taatgtgtat atcaccgaca tgaaaatcac ggaaatcccg cagaaataag gtatatcccc
    29701 ccgggaaatc cccggggatg acggagataa tgacatgcac agtcagttaa aagaacgtat
    29761 ccggctgatg cgcgcaaggc tggataacgc cgcgccggtt gctgaaatcc gggctgaatc
    29821 tcagcttttt gtgactcctg ccccggtctg tgatcgcctg gtgacgctgg cggagatcag
    29881 caaccgtgat cacattctgg aaccctctgc cggcaccggg gccatactgc gggcgattcg
    29941 tcatgtcgcg ccgggggcca tgtgtgatgc ggtggagatt aacagcgggc tggtccggta
    30001 tctgcgggat aattttaacg gtgtcagggt tcagtgtggc gacttcatgg aatggcagcc
    30061 ggtgcagtat tacagccgga ttatcatgaa tccgccgttc agtcacgggc aggatatccg
    30121 gcatatcctg cgggcttttt ccctgttgcg tccgggtggt gtgctggttg ccgtctgtct
    30181 gaacgggccg cgccagcagg agaagctgtt accgttttct gacgtccgcg aggagctgcc
    30241 acgcggcacg tttgcttata cccgtgttcc gacgatgatt attcgtctgc gggcctgatg
    30301 ac
//
LOCUS       DAZKHK010000059        30129 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKHK010000059 DAZKHK010000000
VERSION     DAZKHK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.059"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHOS5_002625"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHOS5_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9777417.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHOS5_002626"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHOS5_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9777418.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHOS5_002627"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHOS5_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9777419.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOS5_002628"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOS5_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9777420.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOS5_002629"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOS5_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9777421.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2978..3475
                     /gene="rplJ"
                     /locus_tag="ACHOS5_002630"
     CDS             2978..3475
                     /gene="rplJ"
                     /locus_tag="ACHOS5_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9777422.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3542..3907
                     /gene="rplL"
                     /locus_tag="ACHOS5_002631"
     CDS             3542..3907
                     /gene="rplL"
                     /locus_tag="ACHOS5_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9777423.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4227..8255
                     /gene="rpoB"
                     /locus_tag="ACHOS5_002632"
     CDS             4227..8255
                     /gene="rpoB"
                     /locus_tag="ACHOS5_002632"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9777424.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8332..12555
                     /gene="rpoC"
                     /locus_tag="ACHOS5_002633"
     CDS             8332..12555
                     /gene="rpoC"
                     /locus_tag="ACHOS5_002633"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9777425.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12796..13929)
                     /gene="thiH"
                     /locus_tag="ACHOS5_002634"
     CDS             complement(12796..13929)
                     /gene="thiH"
                     /locus_tag="ACHOS5_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP9777426.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVVEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(13926..14696)
                     /gene="thiG"
                     /locus_tag="ACHOS5_002635"
     CDS             complement(13926..14696)
                     /gene="thiG"
                     /locus_tag="ACHOS5_002635"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP9777427.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTVEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEKLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASE"
     gene            complement(14698..14898)
                     /gene="thiS"
                     /locus_tag="ACHOS5_002636"
     CDS             complement(14698..14898)
                     /gene="thiS"
                     /locus_tag="ACHOS5_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP9777428.1"
                     /translation="MQILFNDQPMQCVAGLTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14882..15637)
                     /gene="thiF"
                     /locus_tag="ACHOS5_002637"
     CDS             complement(14882..15637)
                     /gene="thiF"
                     /locus_tag="ACHOS5_002637"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP9777429.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGEALKDVVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGCNADPV"
     gene            complement(15630..16265)
                     /gene="thiE"
                     /locus_tag="ACHOS5_002638"
     CDS             complement(15630..16265)
                     /gene="thiE"
                     /locus_tag="ACHOS5_002638"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP9777430.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16265..18160)
                     /gene="thiC"
                     /locus_tag="ACHOS5_002639"
     CDS             complement(16265..18160)
                     /gene="thiC"
                     /locus_tag="ACHOS5_002639"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP9777431.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITATQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGHRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18241..18341)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18393..18869)
                     /gene="rsd"
                     /locus_tag="ACHOS5_002640"
     CDS             complement(18393..18869)
                     /gene="rsd"
                     /locus_tag="ACHOS5_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP9777432.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            18964..19737
                     /gene="nudC"
                     /locus_tag="ACHOS5_002641"
     CDS             18964..19737
                     /gene="nudC"
                     /locus_tag="ACHOS5_002641"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP9777433.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEAHWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19777..20841
                     /gene="hemE"
                     /locus_tag="ACHOS5_002642"
     CDS             19777..20841
                     /gene="hemE"
                     /locus_tag="ACHOS5_002642"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP9777434.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20851..21522
                     /gene="nfi"
                     /locus_tag="ACHOS5_002643"
     CDS             20851..21522
                     /gene="nfi"
                     /locus_tag="ACHOS5_002643"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP9777435.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTIPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21565..22155
                     /gene="yjaG"
                     /locus_tag="ACHOS5_002644"
     CDS             21565..22155
                     /gene="yjaG"
                     /locus_tag="ACHOS5_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP9777436.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22342..22614
                     /gene="hupA"
                     /locus_tag="ACHOS5_002645"
     CDS             22342..22614
                     /gene="hupA"
                     /locus_tag="ACHOS5_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP9777437.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22627..23322
                     /gene="yjaH"
                     /locus_tag="ACHOS5_002646"
     CDS             22627..23322
                     /gene="yjaH"
                     /locus_tag="ACHOS5_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP9777438.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23324..23743)
                     /gene="zraP"
                     /locus_tag="ACHOS5_002647"
     CDS             complement(23324..23743)
                     /gene="zraP"
                     /locus_tag="ACHOS5_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP9777439.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINALAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGDCGGGGHMGMGHW"
     gene            23981..25357
                     /gene="zraS"
                     /locus_tag="ACHOS5_002648"
     CDS             23981..25357
                     /gene="zraS"
                     /locus_tag="ACHOS5_002648"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP9777440.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMMGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERASAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25354..26679
                     /gene="zraR"
                     /locus_tag="ACHOS5_002649"
     CDS             25354..26679
                     /gene="zraR"
                     /locus_tag="ACHOS5_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP9777441.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVAQANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLANHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26676..27965)
                     /gene="purD"
                     /locus_tag="ACHOS5_002650"
     CDS             complement(26676..27965)
                     /gene="purD"
                     /locus_tag="ACHOS5_002650"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9777442.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27977..29566)
                     /gene="purH"
                     /locus_tag="ACHOS5_002651"
     CDS             complement(27977..29566)
                     /gene="purH"
                     /locus_tag="ACHOS5_002651"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9777443.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DANEGSLLLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGHSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7058 a         7812 c         8294 g         6965 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggtgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggtc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa tacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact atctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagagttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgttgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggcgttgttg aagaagttga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga cccctgtaga gctggacttc agccaggttg
     1381 aaaaagctta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgcgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcactg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgctctgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 gggtgcaaat gctgaagctg ctaaagctgc aggtgctgaa ctggtaggta tggaagatct
     2281 ggctgaacag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggcccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 gattcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtagactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctctacca ctatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtatgctaac gaaagttagc agctaaaaag
     2761 atttgttcgt tggagcctgg cctatccagg cctccgtcga agaccgcagg agtttcgcaa
     2821 gaaacttaat cccctgcgta gacggtgaca gaacgctaag attattcttt tatattctgg
     2881 cttgtttctg ctcaccgtaa ttaagacgct ctctccgttt ggaggagtga agtgagttcc
     2941 agagattttc tctggcaaac atccaggagc aaagctaatg gctttaaatc ttcaagacaa
     3001 acaagcgatt gttgctgaag tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc
     3061 ggattcccgt ggcgtaactg tagataaaat gactgaactg cgtaaagcag gtcgcgaagc
     3121 tggcgtatac atgcgtgttg ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc
     3181 gttcgagtgc ctgaaagacg cgtttgttgg tccgaccctg attgcatact ctatggaaca
     3241 cccgggcgct gctgctcgtc tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt
     3301 caaagccgct gcctttgaag gtgagctgat cccggcgtct cagatcgacc gcctggcaac
     3361 tctgccgacc tacgaagaag caattgcacg cctgatggca accatgaaag aagcttcggc
     3421 tggcaaactg gttcgtactc tggctgctgt acgcgatgcg aaagaagctg cttaatcgca
     3481 gttatctttt taacgcattc gcttacgtat aaacttattc tgatattcag gaacaattta
     3541 aatgtctatc actaaagatc aaatcattga agcagttgca gctatgtctg taatggacgt
     3601 tgtagaactg atctctgcaa tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc
     3661 tgtagctgct ggcccggttg aagctgctga agaaaaaact gaattcgacg taattctgaa
     3721 agctgctggc gctaacaaag ttgctgttat caaagcagta cgtggcgcaa ctggcctggg
     3781 tctgaaagaa gctaaagacc tggttgaatc tgcaccggct gctctgaaag aaggcgtgag
     3841 caaagacgac gcagaagcac tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt
     3901 taaataagcc aacccttccg gttgcagcct gagaaatcag gctgatggct ggtgactttt
     3961 tagtcaccag cctttttgcg ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg
     4021 ctgtgccttt caatgcttgt ttctatcgac gacttaatat actgcgacag gacgcccgtt
     4081 ctgtgtaaat cgcaatgaaa tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt
     4141 ccattttccg gtcaacaaaa tagtgttgca caaactgtcc gctcaatgga cagatgggtc
     4201 gacttgtcag cgagctgagg aaccctatgg tttactccta taccgagaaa aaacgtattc
     4261 gtaaggattt tggtaaacgt ccacaagttc tggatgtacc ttatctcctt tctatccagc
     4321 ttgactcgtt tcagaaattt atcgagcaag atcctgaagg gcagtatggt ctggaagctg
     4381 ctttccgttc cgtattcccg attcagagct acagcggtaa ttccgagctg caatacgtca
     4441 gctaccgcct tggcgaaccg gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct
     4501 attccgcacc gctgcgcgtt aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag
     4561 gcaccgtaaa agacattaaa gaacaagaag tctacatggg cgaaattccg ctcatgacgg
     4621 acaacggtac ctttgttatc aacggtactg agcgtgttat cgtttcccag ttgcaccgta
     4681 gtccgggggt cttttttgac tccgacaaag gtaaaaccca ctcttcgggt aaggtgctgt
     4741 ataacgcgcg catcatccct taccgtggtt cctggctgga cttcgaattc gatccgaagg
     4801 acaacctgtt cgtacgtatc gaccgtcgcc gtaaactacc tgcgaccatc attctgcgtg
     4861 ccctgaacta caccacagag cagatcctcg acctgttctt tgaaaaagtt atctttgaaa
     4921 tccgtgataa caagctgcag atggaactgg tgccggaacg cctgcgtggt gaaaccgcat
     4981 cttttgacat cgaagctaac ggtaaagtgt acgtagaaaa aggccgccgc attactgcgc
     5041 gccatattcg ccagctggaa aaagacgacg tcaagctgat cgaagtcccg gttgagtaca
     5101 tcgcaggtaa agtggttgct aaagactaca ttgatgagtc taccggcgag ctgatctgcg
     5161 cagcgaacat ggagctgagc ctggatctgc tggctaagct gagccagtct ggtcataagc
     5221 gtatcgaaac gctgttcacc aacgatctgg atcacggccc atatatctct gaaaccttac
     5281 gtgtcgaccc aactaacgac cgtctgagcg cactggtaga aatctaccgt atgatgcgcc
     5341 ctggcgagcc gccgactcgt gaagctgctg aaagcctgtt cgagaacctg ttcttctccg
     5401 aagaccgcta tgacttgtct gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg
     5461 aagaaatcga aggttccggt atcctgagca aagacgacat cattgatgtt atgaaaaagc
     5521 tcattgatat ccgtaacggt aaaggcgaag tcgatgatat cgaccacctc ggcaaccgtc
     5581 gtatccgttc cgttggcgaa atggcggaaa accagttccg cgttggcctg gtacgtgtag
     5641 agcgtgcggt gaaagagcgt ctgtctctgg gcgatctgga taccctgatg cctcaggata
     5701 tgatcaacgc caagccgatt tccgcagcag tgaaagagtt cttcggttcc agccagctgt
     5761 ctcagtttat ggaccagaac aacccgctgt ctgagattac gcacaaacgt cgtatctccg
     5821 cactcggccc aggtggtctg acccgtgaac gtgcaggctt cgaagttcga gacgtacacc
     5881 cgactcacta cggtcgcgta tgtccaatcg aaacccctga aggtccgaac attggtctga
     5941 tcaactccct gtctgtatac gcgcagacta acgaatacgg cttccttgag actccgtatc
     6001 gtaaagtgac cgacggtgtt gtaactgacg aaattcacta cctgtctgct atcgaagaag
     6061 gcaactacgt tatcgcccag gcgaactcca acctggatga agaaggccac ttcgtagaag
     6121 acctggtaac ttgccgtagc aaaggcgaat ccagcttgtt cagccgtgac caggttgact
     6181 acatggacgt atccacccag caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg
     6241 aacacgatga cgccaaccgt gcattgatgg gtgcgaacat gcaacgtcag gccgttccga
     6301 ctctgcgcgc tgataagccg ctggttggta cgggtatgga acgtgctgtt gccgttgact
     6361 ccggtgtaac tgcggttgct aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta
     6421 tcgttatcaa agttaacgaa gacgagatgt atccgggtga agcaggtatc gacatctaca
     6481 acctgaccaa atacacccgt tctaaccaga acacctgtat caaccagatg ccgtgtgtgt
     6541 ctctgggtga accggtagaa cgtggcgacg tgctggcaga cggtccgtcc accgacctcg
     6601 gtgaactggc gcttggtcag aacatgcgcg tagcgttcat gccgtggaat ggttacaact
     6661 tcgaagactc catcctcgta tccgagcgtg ttgttcagga agaccgtttc accaccatcc
     6721 acattcagga actggcgtgt gtgtcccgtg acaccaagct ggggccagaa gagattaccg
     6781 ctgacatccc gaacgtgggt gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt
     6841 acattggtgc ggaagtaacc ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg
     6901 aaacccagct gaccccagaa gaaaaactgc tgcgtgcgat cttcggtgag aaagcgtctg
     6961 acgttaaaga ctcttctctg cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc
     7021 aggtctttac tcgcgatggc gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc
     7081 agctcaaaca ggcgaagaaa gacctgtctg aagaactgca gatcctcgaa gcgggtctgt
     7141 tcagccgtat ccgtgctgtg ctggtagccg gtggcgttga agctgagaag ctcgacaaac
     7201 tgccgcgcga tcgctggctg gagctgggcc tgaccgacga agagaaacaa aatcagctgg
     7261 aacagctggc tgagcagtat gacgaactga aacacgagtt cgagaagaaa ctcgaagcga
     7321 aacgccgcaa aatcacccag ggcgacgatc tggcaccggg cgtgctgaag attgttaaag
     7381 tatatctggc ggttaaacgc cgtatccagc ctggtgacaa gatggcaggt cgtcacggta
     7441 acaagggtgt aatttctaag atcaacccga tcgaagatat gccttacgat gaaaacggta
     7501 cgccggtaga catcgtactg aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga
     7561 tcctcgaaac ccacctgggt atggctgcga aaggtatcgg cgacaagatc aacgccatgc
     7621 tgaaacagca gcaagaagtc gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg
     7681 gcgctgacgt tcgtcagaaa gttgacctga gtaccttcag cgatgaagaa gttatgcgtc
     7741 tggctgaaaa cctgcgcaaa ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaaag
     7801 aagcagaaat taaagagctg ctgaaacttg gcgacctgcc gacttctggt cagatccgcc
     7861 tgtacgacgg tcgcactggt gaacagttcg aacgtccggt aaccgttggt tacatgtaca
     7921 tgctgaaact gaaccacctg gtcgacgaca agatgcacgc gcgttccacc ggttcttaca
     7981 gcctggttac tcagcagccg ctgggtggta aggcacagtt cggtggtcag cgtttcgggg
     8041 agatggaagt gtgggcgctg gaagcatatg gcgcagcata caccctgcag gaaatgctca
     8101 ccgttaagtc tgatgacgtg aacggtcgta ctaagatgta taaaaacatc gtggacggca
     8161 accatcagat ggagccgggc atgccagaat ccttcaacgt attgttgaaa gagattcgtt
     8221 cgctgggtat caacatcgaa ctggaagacg agtaattctc gctcaaacag gtcactgctg
     8281 tcggggtaaa acccggcagc ggattgtgct aactccgacg ggagcaaatc cgtgaaagat
     8341 ttattaaagt ttctgaaagc gcagactaaa accgaagagt ttgatgcgat caaaattgct
     8401 ctggcttcgc cagacatgat ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc
     8461 atcaactacc gtacgttcaa accagaacgt gacggccttt tctgcgcccg tatctttggg
     8521 ccggtaaaag attacgagtg cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc
     8581 atctgtgaga agtgcggcgt tgaagtgacc cagactaaag tacgccgtga gcgtatgggc
     8641 cacatcgaac tggcttcccc gactgcgcac atctggttcc tgaaatcgct gccgtcccgt
     8701 atcggcctgc tgctcgatat gccgctgcgc gatatcgaac gcgtactgta cttcgaatcc
     8761 tatgtggtta tcgaaggcgg tatgaccaac ctggaacgtc agcagatcct gactgaagag
     8821 cagtatctgg acgcgctgga agagttcggt gacgaattcg acgcgaagat gggggcggaa
     8881 gcaatccagg ccctgctgaa gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa
     8941 gagctgaacg aaaccaactc cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg
     9001 ctggaagcgt tcgttcagtc tggtaacaaa ccagagtgga tgatcctgac cgttctgccg
     9061 gtactgccgc cagatctgcg tccgctggtt ccgctggatg gtggtcgttt cgcgacttct
     9121 gacctgaacg atctgtatcg tcgcgtcatt aaccgtaaca accgtctgaa acgactgctg
     9181 gacctggctg cgccggatat catcgtacgt aacgaaaaac gtatgctgca ggaagcggta
     9241 gacgccctgc tggataacgg tcgtcgcggt cgtgcaatca ccggttctaa caagcgtcct
     9301 ctgaaatctt tggccgacat gatcaaaggt aaacagggtc gtttccgtca gaacctgctc
     9361 ggtaagcgtg ttgactactc cggtcgttct gtaatcaccg taggtccata cctgcgtctg
     9421 catcagtgcg gtctgccgaa gaaaatggcg ctggagctgt tcaaaccgtt catctacggc
     9481 aagctggaac tgcgtggtct tgctaccacc atcaaagctg cgaagaaaat ggttgagcgc
     9541 gaagaagctg tcgtttggga tatcctggac gaagttatcc gcgaacaccc ggtactgctg
     9601 aaccgtgcac cgactctgca ccgtctgggt atccaggcat ttgaaccggt actgatcgaa
     9661 ggtaaagcta tccaactgca cccgctggtt tgtgcggcat ataacgccga cttcgatggt
     9721 gaccagatgg ctgttcacgt accgctgacg ctggaagccc agctggaagc gcgtgcgctg
     9781 atgatgtcta ccaacaacat cctgtccccg gcgaacggcg aaccaatcat cgttccgtct
     9841 caggacgttg tactgggtct gtactacatg acccgtgact gtgttaacgc caaaggcgaa
     9901 ggcatggtgc tgactggccc gaaagaagca gaacgtctgt atcgctctgg tctggcttct
     9961 ctgcatgcgc gcgttaaagt gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta
    10021 gtagcgaaaa ccagcctgaa agacacgact gttggccgtg ccattctgtg gatgattgta
    10081 ccgaaaggtc tgccttacac catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa
    10141 atgctgaaca cctgctaccg cattctcggt ctgaaaccga ccgttatttt tgcggaccag
    10201 atcatgtata ccggctttgc atatgcagcg cgttctggtg catctgttgg tatcgatgac
    10261 atggtcatcc cggagaagaa acacgaaatc atctccgagg cagaagcaga agttgctgaa
    10321 attcaggagc agttccagtc tggtctggta actgcgggcg aacgctacaa caaagttatc
    10381 gatatctggg ctgcggcgaa cgatcgtgta tccaaagcga tgatggataa cctgcaaact
    10441 gaaaccgtta ttaaccgtga cggtcaggaa gagaagcagg tttccttcaa cagcatctac
    10501 atgatggccg actccggtgc gcgtggttct gcggcacaga ttcgtcagct tgctggtatg
    10561 cgtggtctga tggcgaagcc ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc
    10621 cgtgaaggtc tgaacgtact ccagtacttc atctccaccc acggtgctcg taaaggtctg
    10681 gcggataccg cactgaaaac ggcgaactcc ggttacctga ctcgtcgtct ggttgacgtg
    10741 gcgcaggacc tggtggttac cgaagacgat tgtggtaccc atgaaggtat catgatgact
    10801 ccggttatcg agggtggtga cgttaaagag ccgctgcgcg atcgcgtatt gggtcgtgta
    10861 actgctgaag acgttctgaa gccgggtact gctgatatcc tcgttccgcg caacacgctg
    10921 ctgcacgaac agtggtgtga cctgctggaa gagaactctg tcgacgcggt taaagtacgt
    10981 tctgttgtat cttgtgacac cgactttggt gtatgtgcgc actgctacgg tcgtgacctg
    11041 gcgcgtggcc acatcatcaa caaaggtgaa gcaatcggtg ttatcgcggc acagtccatc
    11101 ggtgaaccgg gtacacagct gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt
    11161 gcggctgctg aatccagcat ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg
    11221 aagtcggttg tgaactccag cggtaaactg gttatcactt cccgtaacac cgaactgaaa
    11281 ctgatcgacg aattcggtcg taccaaagaa agctacaaag taccttacgg tgcggtactg
    11341 gcgaaaggcg atggcgaaca ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac
    11401 accatgccgg ttatcaccga agtaagcggt tttgtacgct ttactgacat gatcgacggc
    11461 cagaccatta ctcgtcagac cgacgaattg acaggtctgt cttcgctggt ggttctggat
    11521 tccgcagaac gtaccgcagg tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct
    11581 cagggtaacg acgttctgat cccgggtacc gatatgcctg cgcagtactt cctgccgggt
    11641 aaagcgattg ttcagctgga agatggcgta cagatcagct ctggtgacac cctggcgcgt
    11701 attccgcagg aatccggcgg taccaaggac atcaccggtg gtctgccgcg cgttgcggac
    11761 ctgttcgaag cacgtcgtcc gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt
    11821 tccttcggta aagaaaccaa aggtaaacgt cgtctggtta tcaccccggt agacggtagc
    11881 gatccgtacg aagagatgat tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt
    11941 gtagaacgtg gtgacgtaat ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg
    12001 cgtggtgttc atgctgtgac tcgttacatc gttaacgaag tacaggacgt ataccgtctg
    12061 cagggcgtta agattaacga taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa
    12121 gctaccatcg ttaacgcggg cagctccgac ttcctggaag gtgaacaggt tgaatactct
    12181 cgcgtcaaga tcgcaaaccg cgaactggaa gcgaacggca aagtaggtgc aacttactcc
    12241 cgcgatctgc tgggtatcac caaagcgtct ctggcaaccg agtccttcat ctccgcggca
    12301 tcgttccagg agaccactcg cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa
    12361 ctgcgcggcc tgaaagagaa cgttatcgtg ggtcgtctga tcccggcagg taccggttat
    12421 gcgtaccacc aggatcgtat gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag
    12481 gtgactgcag aagacgcatc tgccagcctg gcagaactgc tgaacgcagg tctgggcggt
    12541 tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
    12601 ttatgggtgt tattcagagg gcaaagtctt cacggaattt ttaagaaagg cagggcgaaa
    12661 cgaggaagaa gcttttcgcc ctggacaaca ttcctgttga cgggactacc ataaaaaaac
    12721 acaacgcttt caacaacctt gcgtttttca tcttccggag ctgctcggaa taaatttacg
    12781 ttcttacatt ctgtctcatg gcctttgcga tgggcgtccc agatagctgt cccagtcttt
    12841 ccataccggc tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc
    12901 gtcgtgcggt gagaactgtt ccagctcggg gtggttgtcg gcatagccac ctggctgcgt
    12961 tttcgaaaaa gcgctgacgt tattaattgc cagcggaatt acgcgatcgc gaaaccacgg
    13021 tgattcccgc gtggagagtg acagttcaat ctccggtgca agcagccgga aagcgcagat
    13081 ggcttgcact aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacatgg
    13141 acgcaggcgt gggaaggaga cagagtagcg gctttgccag taatgctgtt gcagccatag
    13201 caaatgttcg acaaccatat agcagtcaac tcgccagctg tcggaaaggc cgattagcgc
    13261 gccgaggcct atcttatcaa tccccgcacg ccctagccga tccggcgttt ccagccgcca
    13321 gaagaagtcc tgttttttac ctttcagatg atgatgggca taagtcgcct catgatatgt
    13381 ctcctgataa accattactc catcgaggcc aagctgtttt aactcggcat attccgcctc
    13441 cgccagcggt tgcacttcca tctgtagtga agagaactgt tcacgcaggg cagggagatg
    13501 acgacgaaag taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg
    13561 ttcaaagccc atctcccgta tagcggcgct ttctctggca atatccgctt catccagcgt
    13621 tttgcgcttg atgcggttgc tcatagaaaa tccgcaataa gtacagtcgt tagcgcaaag
    13681 attggaaaga taaagcggga cgtagaagct gacgacgttg ccaaatcgct gacgagtcag
    13741 acgttgcgct cgctgggcca gtggttccag atagccactg gcggcgggtg atagcaatgc
    13801 catcatgtcg tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt
    13861 tttgccgttg atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa
    13921 ggttttcatt ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga
    13981 ctgcggctgc ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat
    14041 gccttcgcca tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg
    14101 tcggccccca tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca
    14161 accaccggaa cggtggcctg ctggataata atctccagca tggcgcgggt ttccagtccc
    14221 tgattcgagc caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga
    14281 cgtttacaca ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc
    14341 agcttttcgg cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg
    14401 tgaatctcta attttaacca gtttgtgcct aacgcttcac gagccagatg ggcggcgaaa
    14461 atggcttctt ccactgtttt cgccccggat gtatttggca gcagggtcac acccgccgcg
    14521 ataagcggtt cgaggatagc gtcgttgtgc tggcgcaagt tgacgcgttt catcgccagt
    14581 gtcaccagtt ggctgccgca agcgcggatc gcctccatca tcagttgtga agaggcgaat
    14641 ttccctgtgc cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca
    14701 accccctgca ataacttgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgc
    14761 ccattgttcc cgcgggacga tttgctgatt aatcgccagt gccgcacctg cttgttgttg
    14821 atcgagttgc tccagcagtt cgtgaacagt tagtccggcg acacactgca tcggttgatc
    14881 gttaaacagg atctgcattg catcctccac ataccgggca accactggcg cggcgcaaag
    14941 ccaggctgcg ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggcgtct
    15001 ctataccgct taataactta atcgcttcca gtgcctgtaa agtgcccata accccgacca
    15061 ccgggccaac cacgcccgcc gtgcggcagt tgcgttctgg ttcctggtta tctggccaca
    15121 ggcagcggta acatccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc
    15181 cgaccgcgct ggcggtgata agcggcgtgt tgagtgctac gcaggtggca ttaatctcct
    15241 ggcgagtcgc catattgtca gtacagtcga gcaccacatc ggcttgtgca accacatctt
    15301 ttaacgcctc acccgtcaac cgttgttgta atgccatcag ttgaatgtcg ggattcaact
    15361 gtgtcagtcg ctgttggctg acctgtgatt tcgggcgatc gatatcttca gtggtaaaga
    15421 ggatttgtcg ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc
    15481 cgacgccagc ccccgccagg tatagcgcag caggtgtacc cagcccgcca agaccaataa
    15541 tcagcacctg gctgtcgagc agtttttgct gcccgtccag agcgatatcg tcgagcagga
    15601 tttggcggct ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc
    15661 aactgtgccg ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac gacggcgata
    15721 ctgccgacac ccgttgctat caccgcaggc gcgcgcgcca gactgatacc gccaatcgcc
    15781 acggtgggat aatccgccag gcgctcaaca tgccgtgcca gctgttccag tccctgcggt
    15841 gcagaaggca tctgtttggt ttgtgtcgga aacacatgtc ccagtgcgat ataagaagga
    15901 cgtgccgcca gcgcgacgtc gatctccata tcgtcatggg tcgaaacgcc cagccgcagg
    15961 cctgccgcgc ggatggcgct gagatcggtg gcttgcaaat cttcctgccc cagatggacg
    16021 ccatacgcct gatgcttaat cgccagccgc cagtaatcgt tgataaacaa tcgcgcgtta
    16081 tagcggcggc ccagcgcaat tgccgccacc acatcggctt ctacctcttc atcgcgctga
    16141 tctttgatgc gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt
    16201 acgctgtcaa ccaccgggta cagtcctaaa cgaaagggta caggaggaaa ctcaggctga
    16261 tacatcacgc ttcctcctta cgcaggtaga tttctccgcc tctggcgcgg aagttctccg
    16321 acatatccgc cattcccact tcaatggttt gcgcggcggc gtaatcacgc acttcctggc
    16381 tgattttcat cgagcagaat ttcggtccac acatggagca aaaatgggcg actttaccgg
    16441 actcttgcgg cagggtttca tcgtgataag cgcgggcagt aaacgggtcg agggccagat
    16501 taaactggtc ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt
    16561 gcgcgcccgg atgccctttc gccaggtcgg cggcgtgagc ggcaatctta taggtgataa
    16621 gcccttgttt aacatcttct ttatttggca ggcccagatg ctctttcggc gttacgtagc
    16681 agagcatcgc gcagccaaac cagccaatca tcgccgcacc aatccctgac gtgaagtggt
    16741 catagcccgg cgcgatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc
    16801 agtgctctaa ctcctcggtc atattgcggc gaatcatctg catcggcacg tggcctgggc
    16861 cttcaatcat cacctgcacg tcatattccc aggcgatttt ggtcagttcg cccagcgtat
    16921 gcagctcggc aaactgtgct tcatcgttgg cgtcctgaat agaaccgggg cgcaggccgt
    16981 cgcccagcga cagcgaaacg tcataagcag cacagatttc acaaatttcg cggaagtgtt
    17041 gatagaggaa attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc
    17101 gagagacgat accggtcagg cgtttcgcgg tcatcggcac gtagcgcagc agcacccccg
    17161 catggatagt gaagtaatcc acaccttgct cggcctgttc cagcagggtg tcgcggaacg
    17221 cttcccaggt aagatcttcg gcgatcccgt tcaccttctc cagtgcctgg tagatcggca
    17281 ctgtaccgat cggcaccggg ctgttacgca aaatccactc gcgggtttcg tgaatatagc
    17341 gaccagtgga aagatccatc acggtatccg ctccccagcg cgtggaccat accagttttt
    17401 ccacttcttc ttcgatggaa gaggtgaccg ctgagttgcc gatattggcg ttaactttta
    17461 ccaggaagtt acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga
    17521 taatcgcacg tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggaa
    17581 gacgtgcgcc aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct
    17641 cgcgacccat attctcgcga atggcgatga attccatttc gggcgtgatg atacccttgc
    17701 gggcgtagtg cagttgtgtg acgcggtggc ctgctttggc gcgttttggc gttaatacgc
    17761 cgctaaaacg cagctcgtcg aggccatcat ctgccagccg cgcttttgtg taatcggaac
    17821 tgcgcacggt aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg
    17881 ccagcccttg ctgcacgtta atggcgatct gcggatcgcc atacggaccg gaggtgtcgt
    17941 agaccggaat cgcttcgttt tcttcgtact gcggttgttc tttgctaccg ccgattagcg
    18001 tcgggctaag ctggatctca cgcatcggca cgcgcacgcc gggttgtgtg gcagtgatgt
    18061 aaatgcgttt tgagttggga aaggcggagc cttccagggt gtcgataaaa tgttgggcct
    18121 gggcgcgttg ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa
    18181 ggacgtggct tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat
    18241 agcagattaa ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga
    18301 ataaacggtc tcagccagtt aaggcactcc gacaagaaat tagccccaca aacggggcgt
    18361 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatatttgac gcgggcggcg
    18421 tcaagcacca gtagaatcag cttatcttcc agcacaaagc gcgcttccag tgcctcgccg
    18481 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccaga
    18541 ctggaatcgt agtcatccat aatctgttga gtattggctt cgagttgtgg ccaaatcttc
    18601 gcggcgcgtg cgagttgccc attgccttcc agcttatgaa gaatgcgctc ataaatactg
    18661 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    18721 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    18781 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    18841 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    18901 gctctgtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    18961 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19021 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19081 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt agtacaactg
    19141 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19201 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19261 tactgcgggc atgaaatgta tccaagcaaa accgaatggg cgatgctgtg cagccattgc
    19321 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19381 tcgatcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19441 ttcgtcgaag tgggtgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19501 ggaattaaag ttaaaaactt gcgttacgtg acctcccaac cgtggccgtt tcctcagtcg
    19561 ttaatgaccg cgtttatggc ggaatatgac agcggcgaga tcgtgatcga cccgaaagaa
    19621 ttgcttgagg cgcactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    19681 gtagcgcgcc gtctgataga agatacagtg gcgatgtgtc gggcagagta tgagtgatga
    19741 tacactgacc gcctgacgca ctaaggaaca gctaaaatga ccgaacttaa aaacgatcgt
    19801 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    19861 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    19921 ctgtgcaaaa atgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctatccg
    19981 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cagacgcgat gggtttaggg
    20041 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgat
    20101 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20161 cgtaccattc gtcgcgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20221 tggacgctgg cgacttacat ggttgaaggc ggtagcagca aagccttcac cgtgatcaaa
    20281 aagatgatgt atgccgatcc gcaggcgctg cacgcgctgc tcgataaact ggcgaaaagc
    20341 gtcacgttgt atctgaatgc gcagattaaa gccggtgctc aggcagtcat gattttcgac
    20401 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgcttta ttacatgcat
    20461 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    20521 accaaaggtg gcggacagtg gctggaagcg atggcagaaa ccggttgcga tgcgctgggc
    20581 ctcgactgga caacggacat cgccgatgcg cgccgtcgcg tgggcaataa agtcgcgctg
    20641 cagggtaata tggacccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagtg
    20701 gcgtctatac ttgccggttt cggtcacggc gaaggccatg tctttaacct tggtcacggc
    20761 attcatcagg atgtgccgcc agaacatgca ggcgtgtttg tggaggcagt gcatcgcttg
    20821 tctgaacagt atcaccgcta aggagcgatt atggatctcg cgtcattacg cgctcaacaa
    20881 attgaactgg cttcttctgt gatccgcgag gatcgactcg ataaagatcc gccggatctg
    20941 atcgccggag ccgatgtcgg atttgagcag ggcggcgaag tgacgcgagc ggcgatggtg
    21001 ctgctgaaat acccctcgct tgagctggtg gagtataaag tggcccgcat cgccaccacc
    21061 atcccttaca ttcctggttt tctctccttt cgtgaatatc ctgcgctgct ggcagcgtgg
    21121 gagatgctgt cacaaaagcc ggatttagtg tttgtcgatg gtcatgggat ctcgcatcct
    21181 cgccgtcttg gcgtcgccag ccattttggt ttgctggtgg atgtgccgac cattggcgta
    21241 gcgaaaaaac ggctctgcgg taaattcgaa ccgctctcca gcgaaccggg cgcgctggcc
    21301 ccgctgatgg ataaaggcga gcagctggcc tgggtctggc gcagcaaagc gcgctgtaac
    21361 ccgttgttta tcgctaccgg ccatcgggtc agcgtggaca gcgcgctggc gtgggtacaa
    21421 cgctgcatga aaggctatcg tctgccggag ccaacgcgct gggcggacgc ggtggcctcg
    21481 gaacgtccgg cgttcgtgcg ctatacagca aatcagccct aattcaggta aactgcggat
    21541 aatttccgta tttgagaact caacatgtta caaaacccaa ttcatctgcg tctggagcgc
    21601 ctggaaagct ggcagcacgt cacttttatg gcctgcttat gcgaacgcat gtaccctaat
    21661 tacgccgtat tctgtcagca aactggtttt ggtgatgggc agatttaccg ccgcattctc
    21721 gatctcattt gggaaacgct gaccgtcaaa gacgcaaaag taaatttcga cagccaactg
    21781 gagaaatttg aagaagcgat tccttcagcc gacgattttg atctgtacgg cgtttatccg
    21841 gcaatcgatg catgcgtggc gttaagtgaa ctggtccatt cgcgtttaag tggcgaaacg
    21901 ctcgaacacg cggtggaagt gagtaagact tccatcacga ccgtggcgat gctggaaatg
    21961 actcaggctg gtcgcgaaat gagcgatgaa gagctcaaag aaaacccagc tgtagagcaa
    22021 gaatgggaca ttcagtggga aatattccga cttttagctg agtgcgaaga acgcgatatc
    22081 gagctgataa aaggccttag ggcagacctg cgtgaggcgg gtgagagcaa tattggtata
    22141 atttttcagc aataagacca gaaaacgtga tttaacgcct gatttgtcgt acctggagtc
    22201 ttccctttcg ccccccgtct ggtctacatt tggggggcga aaaaaagtgg ctatcggtgc
    22261 gtgtatgcag gagagtgcta ttctggcatt tccgtcgcac tcgatgctta gcaagcgata
    22321 aacacattgt aaggataact tatgaacaag actcaactga ttgatgtaat tgcagagaaa
    22381 gcagaactgt ccaaaaccca ggctaaagct gctctggagt ccactctggc tgcaattact
    22441 gagtctctga aagaaggcga tgctgtacaa ctggttggtt tcggtacctt caaagtgaac
    22501 caccgcgctg agcgtactgg ccgcaacccg cagaccggta aagaaatcaa aattgccgca
    22561 gctaacgtac cggcatttgt ttctggcaag gcactgaaag acgcagttaa gtaagattgc
    22621 gtggcagtga acagttttaa cgaaggggtg gtttcacccc ttttgtcttt ctggcgtcga
    22681 tcattgatgc tggcaggcgc tctgtttctc actgcctgta gtcacaactc ttcacttcct
    22741 ccctttaccg ccagtggatt tgctgaagac cagggcgcgg tacgcatctg gcgaaaagac
    22801 agcggcgata atgtgcatct gcttgccgtg tttagcccgt ggcgcagtgg cgataccacc
    22861 acgcgagagt atcgctggca gggcgataac cttacgctca tcaatatcaa tgtttacagc
    22921 aaaccgccgg tgaacattcg cgcgcgtttt gacgatcgcg gcgatctgag ctttatgcaa
    22981 cgtgaatccg atggggaaaa gcagcagctt tctaacgacc aaatcgattt ataccgttat
    23041 cgtgctgatc agatccgcca gattagcgat gccttacgtc aggggagagt cgtgctgcgc
    23101 caggggcgct ggcatgcgat ggaacagacc gtgaccacct gcgaagggca aaccattaaa
    23161 cctgatttag attcgcaggc gatagcgcat atcgagcgcc gccagagccg ctcttctgtt
    23221 gatgtcagcg tggcatggct ggaagcgccc gaaggttcgc aattactgtt ggtggcaaac
    23281 tctgatttct gtcgctggca acccaacgag aaaacgttct gatttaccag tggcccatac
    23341 ccatatgacc gccaccaccg cagtcgccgt aacccattcc ggtaccgcgc ggaatacccg
    23401 cttcagccat cgcaatatct cgtttcaccc gtaactcatc taacgactga cgcaaattct
    23461 ccatctcttt ggcgagcgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg
    23521 cattgtattc ataacgcttt gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa
    23581 agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt
    23641 gctgccacat accatgtccg ccgtgagcaa atgccgatgt cgatcccatc gccattgctg
    23701 aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat cctccagtgg
    23761 ttgtcttact tcgggtattg catcttccgt gccaacggcg aaacgctgat atgacgggta
    23821 atctggcatg ataaacgagt aaaaatgact cgcctgttgt gggtagcgag tcatttttac
    23881 tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg
    23941 atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc
    24001 taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt
    24061 ggggctgttt gcggtgacag tgattcgcga ttatgggcgc gagactgccg ccgccagaca
    24121 aacgctgctg gaaaaaggca gtgtacttat ccgtgctctc gaatccggct cgcgcgtcgg
    24181 catggggatg cgcatgcatc atgcgcagca gcaggcatta ctggaagaaa tggccgggca
    24241 gcctggtgta cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa
    24301 ctccggcatg atgggaaaac agctttattc cccgcaggaa atgcagcagt tacatccggg
    24361 agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta
    24421 tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata tgcagcagta
    24481 tgccgcgaca ccacaagcaa tttttatcgc tttcgacgcc agtaacattg tgagtgctga
    24541 agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg tcttgctggc
    24601 aagcgtgttg tcattcttct ggtatcgccg atatctgcgc tcgcggcagc tattgcagga
    24661 tgaaatgaag cgcaaagaga agctggtggc actggggcat ctggcggcag gcgttgccca
    24721 cgaaatccgt aacccacttt cctcgattaa agggctggcg aaatactttg ccgaacgcgc
    24781 gtcagcaggg ggagaagcgc atcaattggc gcaggtgatg gcgaaagaag ccgaccgttt
    24841 aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc
    24901 ggtggagctc aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag
    24961 ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc
    25021 ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca
    25081 gcatggcgtg attagcgtga cggccagcga aagcggcgcg ggcgtgaaaa tcagcgttac
    25141 cgacagcggt aagggaattg cggcggatca gcttgaagcc atcttcaccc cgtacttcac
    25201 caccaaagcc gaaggcaccg gactggggct ggcggtcgtg cataatattg ttgaacaaca
    25261 cggtggtaca attcaggtcg caagccagga gggaaaaggc gcaacgttca ccctttggct
    25321 tccggtcaat attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg
    25381 gtggtggatg atgacattag ccactgcact attttgcagg ctttgctgcg cggctggggc
    25441 tataacgtcg cgcaggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt
    25501 tttgatcttg tgctttgcga tgtgcgaatg gcagagatgg acggcatcgc cacgctcaaa
    25561 gagatcaaag cgttaaatcc ggcaattccg gtactgatta tgaccgcgta ctccagcgtc
    25621 gagacggcgg tagaggcgtt gaaaaccggg gcgctggatt atctcatcaa gccgctggat
    25681 ttcgataacc tacaggcgac gctggaaaag gcgctcgccc atacacacag tgttgacgct
    25741 gaaacgcctg cggtgtctgc cagccagttc ggtatggtcg gtaaaagccc ggcgatgcaa
    25801 cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacggtgct gatccacggc
    25861 gattccggca ccggtaaaga gctggtcgcc agggcgattc acgccagtag cgcacgtagc
    25921 gaaaaaccgc tggtaacgct caactgcgcg gcgctcaacg aatccttact ggagtctgaa
    25981 ttgttcggtc acgaaaaagg ggcgtttacc ggggccgaca aacggcggga ggggcgcttt
    26041 gttgaggcgg acggcggcac gttgtttctc gatgaaattg gcgatatctc gccgatgatg
    26101 caggtgcgtc tgctacgtgc gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag
    26161 actatttcgg ttgatgtccg gctgattgcg gcgactcatc gcgatcttgc cgcagaggtg
    26221 aatgccgggc gttttcgcca ggatctctac tatcgtctga acgtggtggc gattgaagtt
    26281 ccgtcgctgc gtcagcggcg ggaagatatt cctctgctgg caaaccattt tcttcagcgc
    26341 tttgccgagc gtaatcgcaa ggcggtaaaa ggttttacgc cccaggcgat ggatctgctg
    26401 attcactacg actggccggg aaatattcgt gaactggaaa acgcggtgga acgggcggtg
    26461 gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgccagtacg
    26521 ccgatcccgc tggtacaaag tcaggatatt cagccgctgg tggaagtcga aaaagaggtg
    26581 attctggcgg cgctggagaa aacgggcggc aacaaaaccg aagccgcccg ccagttaggg
    26641 atcacgcgca aaacgctatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagcg
    26701 cgccagccga tatctttccg gcagaagcag tcgtcccaat ggatatcggt cattaaggca
    26761 taagcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtaacgca cagtacgcgc
    26821 ccgccgctgg tcactacctg ctcgtcatcc gctagtttcg tgcccgcgtg gaacactttg
    26881 ccgtctgcca cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc
    26941 ggatatccac ccgcagccat caccacgccg agagaagcgc gttcatccca ctcggacgtt
    27001 ttctcgtcca gcttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg
    27061 cgcagcataa tcggctgggt ttccggatcg ccaaagcggc agttaaattc aataaccttc
    27121 ggattgccct gtttgtcgat catcaggccc gcgtagagaa aaccagtgta ggtgttgcct
    27181 tccgccgcca tgcctttcac ggttggccag atgatacgtt ccatcgtgcg ctgatgaacc
    27241 tcatcggtca ctactggcgc aggggagtaa gcgcccatcc cgccggtgtt cggcccggta
    27301 tctttatcgc ctacgcgttt gtgatcctgg ctggtggcca tcggcagcac atgctcgccg
    27361 tccaccatca cgataaagct cgcttcttcg ccgtcgagga actcttcgat aacaatgcga
    27421 tgacccgcgt cgccaaacgc gttgcccgcc agcatgtcgc gcacggcggc ttcagcttct
    27481 tccagcgtca tcgccacgat aacgcctttc ccggcggcca gaccgtccgc tttaatgacg
    27541 attggcgcgc ctttctcacg cagatacgcc agcgcaggtt ctacctcggt gaagttctgg
    27601 tattccgctg tgggaatgtt atggcgggcc agaaagtctt tggtgaatgc tttagaacct
    27661 tccagttgtg cagcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggta
    27721 tcgactacgc ctttcaccag cggcgcttcc gggccgacga tggttagatc taccttttcg
    27781 ttttgtgcga aatccagcag cgccgggata tcggttacgc cgatagcgac gttctgtagc
    27841 gcgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtttc aaccagcggc
    27901 gactgagccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact
    27961 ttcatctatt gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc attgcaatac
    28021 cgtgctcgtc ggcggcggca atcacttcgt catcacggat agaaccgcca ggctggatta
    28081 cgcaggtcac gcccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt
    28141 cagaagccat cgaggaacct ttcacttcca gaccttcatc ggcagcttta ataccggcga
    28201 tttttgcgga gtacacgcgg ctcatctggc ccgcgccaat gccgatggtc atattgtttt
    28261 tggcatagac aatggcgttg gatttcacga atttcgccac cttccagcag aacagcgcat
    28321 cacgcaactc ctgttcgctc ggctggcgtt tggtgacgac gcgcagttct tccgcaccga
    28381 ccatgcccag gtcgcgatcc tgaaccagga ggccgccgtt cacgcgtttg aaatcaaggc
    28441 ccggaacacg ctcgccccac tgaccgcagg tcagaacgcg tacgttctgt ttggcggcgg
    28501 tgattttcag agcttcttcg ctggcggacg gcgcaataat cacttcgaca aactgacgag
    28561 aaatgatggc ctgtgcggtt tccgcatcca gctcgcggtt aaaggcgata atgccgccga
    28621 acgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaatggccga
    28681 tagccacgcc gcaagggttg gcgtgcttca caatcacaca tgccggctcg gcgaactctt
    28741 tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gctttaccct
    28801 gaacctgggt tgcggtagca acggaggctt ctttcacatt ctcttctata tagaaggcag
    28861 cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca
    28921 gcgtgcgtgg gaagcgaccg gcggcttctt tgctttcacc gtggtaagcc ggaaccatgc
    28981 tgccgaagta gttggcaatc atgctgtcgt aggcggcggt atgttcaaag gctttgatag
    29041 cgagatcgaa acgggttgca agcagaagcg atccttcgtt ggcatccatc tctttaatga
    29101 tggcgtcgta gtcgctgctc ttcaccacga tggcgacatc tttatggttc ttggcggcgg
    29161 agcgcaccat cgttgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcagc
    29221 cttcacgggc aacggtctgg gcgaacggat acaggttaac aaccaccata tcgataggct
    29281 ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga attccaccat
    29341 gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat
    29401 cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggcagtg ccccctgtag
    29461 acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt
    29521 cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg
    29581 atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct
    29641 aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga
    29701 atttttctct ttcaatggtg atcacaattt tgacagtggc taccgtggga aaaatacaga
    29761 aattacattg atgattgtgg ataactctgt gtgtaaaaag gtataaagcg gggttttgct
    29821 ggggaatgca gcagtcagtc atttttctgc aatttttcta ttgcggcctg cggagaactc
    29881 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    29941 ggttgaagag aaaaatcctg aagttcaggg ttgactctga aagaggaaag cgtaatatac
    30001 gccacctcgc gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga
    30061 caatctgtgt gggcactcga agatacggat tcttgacgtc gcaagacgaa aaatgaatac
    30121 caagtctca
//
LOCUS       DAZKHK010000060        29432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKHK010000060 DAZKHK010000000
VERSION     DAZKHK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.060"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            232..567
                     /gene="yjdM"
                     /locus_tag="ACHOS5_002652"
     CDS             232..567
                     /gene="yjdM"
                     /locus_tag="ACHOS5_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9777444.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            727..1170
                     /gene="yjdN"
                     /locus_tag="ACHOS5_002653"
     CDS             727..1170
                     /gene="yjdN"
                     /locus_tag="ACHOS5_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP9777445.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            1303..2091
                     /gene="phnC"
                     /locus_tag="ACHOS5_002654"
     CDS             1303..2091
                     /gene="phnC"
                     /locus_tag="ACHOS5_002654"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9777446.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2116..3132
                     /gene="phnD"
                     /locus_tag="ACHOS5_002655"
     CDS             2116..3132
                     /gene="phnD"
                     /locus_tag="ACHOS5_002655"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9777447.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3238..4017
                     /gene="phnE"
                     /locus_tag="ACHOS5_002656"
     CDS             3238..4017
                     /gene="phnE"
                     /locus_tag="ACHOS5_002656"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9777448.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4038..4763
                     /gene="phnF"
                     /locus_tag="ACHOS5_002657"
     CDS             4038..4763
                     /gene="phnF"
                     /locus_tag="ACHOS5_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP9777449.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNLSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4764..5216
                     /gene="phnG"
                     /locus_tag="ACHOS5_002658"
     CDS             4764..5216
                     /gene="phnG"
                     /locus_tag="ACHOS5_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP9777450.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLITPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5285..5797
                     /gene="phnH"
                     /locus_tag="ACHOS5_002659"
     CDS             5285..5797
                     /gene="phnH"
                     /locus_tag="ACHOS5_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP9777451.1"
                     /translation="MSEPGVIVALHQLKRGWQPLNIATTSVLLTLADNDTPVWLAAPL
                     SNDIVNQSLRFHTNAPLVNQPKQATFAVTDEAISSEQLNALSTGTAVAPEAGATLILQ
                     VTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHELTERPHPFPLGIDLILTCGERLLA
                     IPRTTHVEVC"
     gene            5797..6861
                     /gene="phnI"
                     /locus_tag="ACHOS5_002660"
     CDS             5797..6861
                     /gene="phnI"
                     /locus_tag="ACHOS5_002660"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP9777452.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6854..7699
                     /gene="phnJ"
                     /locus_tag="ACHOS5_002661"
     CDS             6854..7699
                     /gene="phnJ"
                     /locus_tag="ACHOS5_002661"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP9777453.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRHFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGKRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            7696..8454
                     /gene="phnK"
                     /locus_tag="ACHOS5_002662"
     CDS             7696..8454
                     /gene="phnK"
                     /locus_tag="ACHOS5_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP9777454.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8565..9245
                     /gene="phnL"
                     /locus_tag="ACHOS5_002663"
     CDS             8565..9245
                     /gene="phnL"
                     /locus_tag="ACHOS5_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP9777455.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLNTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9242..10378
                     /gene="phnM"
                     /locus_tag="ACHOS5_002664"
     CDS             9242..10378
                     /gene="phnM"
                     /locus_tag="ACHOS5_002664"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP9777456.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10378..10935
                     /gene="phnN"
                     /locus_tag="ACHOS5_002665"
     CDS             10378..10935
                     /gene="phnN"
                     /locus_tag="ACHOS5_002665"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP9777457.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDTLLSLIHQKEKHHACL"
     gene            10922..11356
                     /gene="phnO"
                     /locus_tag="ACHOS5_002666"
     CDS             10922..11356
                     /gene="phnO"
                     /locus_tag="ACHOS5_002666"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP9777458.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11358..12116
                     /gene="phnP"
                     /locus_tag="ACHOS5_002667"
     CDS             11358..12116
                     /gene="phnP"
                     /locus_tag="ACHOS5_002667"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP9777459.1"
                     /translation="MSLTFTLTGTGGAQGVPAWGCDCVACARARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12144..12473)
                     /gene="yjdP"
                     /locus_tag="ACHOS5_002668"
     CDS             complement(12144..12473)
                     /gene="yjdP"
                     /locus_tag="ACHOS5_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP9777460.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12542..12991)
                     /gene="rpiB"
                     /locus_tag="ACHOS5_002669"
                     /gene_synonym="alsI"
     CDS             complement(12542..12991)
                     /gene="rpiB"
                     /locus_tag="ACHOS5_002669"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFP9777461.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERDVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQQRN"
     gene            13350..14240
                     /gene="alsR"
                     /locus_tag="ACHOS5_002670"
     CDS             13350..14240
                     /gene="alsR"
                     /locus_tag="ACHOS5_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFP9777462.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14299..15234
                     /gene="alsB"
                     /locus_tag="ACHOS5_002671"
     CDS             14299..15234
                     /gene="alsB"
                     /locus_tag="ACHOS5_002671"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFP9777463.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANARKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15362..16894
                     /gene="alsA"
                     /locus_tag="ACHOS5_002672"
     CDS             15362..16894
                     /gene="alsA"
                     /locus_tag="ACHOS5_002672"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFP9777464.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            16873..17853
                     /gene="alsC"
                     /locus_tag="ACHOS5_002673"
     CDS             16873..17853
                     /gene="alsC"
                     /locus_tag="ACHOS5_002673"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFP9777465.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17864..18559
                     /gene="alsE"
                     /locus_tag="ACHOS5_002674"
     CDS             17864..18559
                     /gene="alsE"
                     /locus_tag="ACHOS5_002674"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFP9777466.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18543..19472
                     /gene="alsK"
                     /locus_tag="ACHOS5_002675"
     CDS             18543..19472
                     /gene="alsK"
                     /locus_tag="ACHOS5_002675"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFP9777467.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLYCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNSGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLLQSCAKVP"
     gene            19745..21730
                     /gene="yjcS"
                     /locus_tag="ACHOS5_002676"
     CDS             19745..21730
                     /gene="yjcS"
                     /locus_tag="ACHOS5_002676"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP9777468.1"
                     /translation="MNNSRLFRLSRIVIAFTAASSMMINTAYATDEVKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIIAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSTKA
                     AGKNISLNFNMSNGDNLNLMLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            21938..22213
                     /gene="ytcA"
                     /locus_tag="ACHOS5_002677"
     CDS             21938..22213
                     /gene="ytcA"
                     /locus_tag="ACHOS5_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP9777469.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            22232..23263
                     /gene="mdtN"
                     /locus_tag="ACHOS5_002678"
     CDS             22232..23263
                     /gene="mdtN"
                     /locus_tag="ACHOS5_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP9777470.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            23263..25314
                     /gene="mdtO"
                     /locus_tag="ACHOS5_002679"
     CDS             23263..25314
                     /gene="mdtO"
                     /locus_tag="ACHOS5_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP9777471.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEAVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINNLQHSITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLHIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGV
                     ATALSHSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            25311..26777
                     /gene="mdtP"
                     /locus_tag="ACHOS5_002680"
     CDS             25311..26777
                     /gene="mdtP"
                     /locus_tag="ACHOS5_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP9777472.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHPLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            26975..29122
                     /gene="fdhF"
                     /locus_tag="ACHOS5_002681"
     CDS             26975..29122
                     /gene="fdhF"
                     /locus_tag="ACHOS5_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:27392..27394,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFP9777473.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
BASE COUNT         6999 a         7993 c         7873 g         6567 t
ORIGIN      
        1 aacatcatgt gaattaaaaa aagaacgagt agagcattaa ccttatctta aataataaat
       61 ggaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
      121 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
      181 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
      241 ccacactgcc caaaatgcaa ctccgaatac acctacgaag ataacggcat gtacatctgc
      301 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
      361 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
      421 ctgaaagtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
      481 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
      541 aagtctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaggccgg ataaggcgtt
      601 tacgccgcat ccggcaacgg tggcgactgc ctgatgcgac gctgaccgcg tcttatcagg
      661 cttccctctc atctgtataa atttcgaact acacttaacg ggcttctctt aactgaggtc
      721 accatcatgc cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc
      781 tattatcaac gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgccg
      841 aaatcagcgc aggacagcgc cgaaaactgc ccttccggaa tgcaatttcc cgataccgcc
      901 atcgcgcatg ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgccatgccg
      961 tcaggaaaag ccagctactc cggctttacg ctggtgctcg attctcaaca ggtggaagaa
     1021 ggaaaacgct ggtttgataa ccttgccgcc aacggaaaaa tcgaaatggc ctggcaggaa
     1081 acgttctggg cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt
     1141 aatgtcatca aacaacagcc aacgcaataa cccgccggga ggcccgccct cccgcactgt
     1201 catcgaattc ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa
     1261 taatccgcca cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt
     1321 gtcgagaagc tcgccaaaac cttcaatcag catcaggcgc tgcacgcggt tgatctgaac
     1381 attcatcatg gtgaaatggt ggctctgctt gggccgtcgg gttccggaaa atccaccctt
     1441 ttacgtcact taagcggttt gattaccggc gataaatctg tcggtagcca tatcgagctg
     1501 ctgggccgca ctgtccagcg cgaaggccgt ctggcccgcg atatccgcaa aagccgcgcc
     1561 cataccggct acatctttca acaattcaac ctggtgaatc gcctgagcgt actggagaac
     1621 gtgctgattg gcgcgctcgg cagcacgcca ttctggcgaa cctgttttag ctacttcacc
     1681 cgcgagcaga aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcactttgcc
     1741 catcaacgcg tttccacact ctccggcggc cagcaacagc gtgtggcgat tgcccgcgcg
     1801 ctgatgcagc aggcgaaggt cattctcgct gacgaaccca tcgcctcgct ggacccggaa
     1861 tcggcacgca tcgtgatgga caccctgcgc gacatcaacc agaacgacgg cattaccgtg
     1921 gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg
     1981 cgccaggggc acgtcttcta cgacggctgc agccaacagt ttgataacga acgttttgac
     2041 catctctacc gcagcattaa ccgcgtcgaa gagaacgcga aagctgcctg acatccccat
     2101 cattgaggaa aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc
     2161 agcctcagca ccctgttaaa tccggcatac gccgaagagc aggaaaaggc gctgaatttc
     2221 ggcattattt caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gttcttacag
     2281 gatatggaga agaagctggg cgtaaaggtc aacgccttct ttgccccgga ctacgcgggc
     2341 attatccagg ggatgcgctt caataaagtg gatatcgcct ggtacggcaa cctgtcggcg
     2401 atggaagcgg tggatcgcgc caacggtcag gtcttcgccc agacggtcgc ggcggatgga
     2461 tcgccgggtt actggagcgt gttgatcgtc aacaaagata gcccgatcaa caacctgaac
     2521 gatctgctgg cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctctacctcc
     2581 ggcttcctcg tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttt
     2641 aagcgcaccg tcaacgccgg gcatgaaacc aacgccctcg ccgtcgctaa caagcaagtg
     2701 gatgtcgcca ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag
     2761 ctgaaagaac tgaaggtgat ctggaaatca ccgctgatcc ctggcgatcc gatcgtctgg
     2821 cgcaaaaatc tttccgaaac cactaaagac aagatctacg acttctttat gaattacggc
     2881 aaaacgccgg aagagaaagc ggtgctggag cgcctgggct gggcgccgtt ccgcgcctcc
     2941 agcgacttgc aactggtgcc gattcgccag ctcgcactgt ttaaagagat gcaaagcgtg
     3001 aaggacaaca aagggctgaa cgaacaggac aagctggcaa aaaccaccgc cattcaggcg
     3061 caactggatg atttggaccg cctgaacaac gcgttaagcg caatgagttc ggtgagtaaa
     3121 gcggtgcagt aaatcgtagg tcggataaga cgccccggcg tcgcatccga caatgtgcag
     3181 acgttgatgc cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg
     3241 caaaccatca ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccgtt
     3301 gttatcgccg tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt
     3361 aaagatggcg gcaacatggc gacgttcgcc gccgacttct tcccgcccga tttcagccag
     3421 tggcaggatt acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg
     3481 ctggcggtgg ttctctccat ccccttcggc ctgatgagcg ctgaaaacct ggtaccgtgg
     3541 tgggtctacc aacctgtacg ccgcctgatg gacgcctgcc gtgccattaa cgaaatggtc
     3601 ttcgccatgc tgttcgtggt cgccgtcggc ctcggcccgt tcgctggcgt gctggcgctg
     3661 tttatccaca ccaccggcgt actttccaaa ctgctttccg aagcggtaga agcgattgaa
     3721 cccggcccgg tggagggcat tcgcgccacc ggtgccaaca agctggaaga gatcctttac
     3781 ggcgtactac cgcaggtcat gccgctgctg atctcctact ctctctatcg cttcgaatcc
     3841 aacgtccgct cggcgaccgt cgtcggtatg gttggtgcgg gcggaattgg cgtcacgctg
     3901 tgggaagcga ttcgcggttt ccagttccag caaacctgcg ccctgatggt gctgattatc
     3961 gtcacggtca gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgatta
     4021 gcgaggcatt gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc
     4081 aggagatagc cgcaaaactt gagcaggagc ttcgtcaaca ctaccgctgc ggcgactatc
     4141 ttcccgccga gcagcaactg gcggcgcgct ttgaggtgaa ccgccacacc ctgcgccgcg
     4201 ccatcgacca actggtggaa aaaggctggg tacagcgccg tcagggcgtc ggcgtactgg
     4261 tgctgatgcg cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc
     4321 tggaccaggg cagccatccc accagcgaaa aattgctatc ggtgctgcgc cccgcctccg
     4381 gtcacgtcgc tgacgcgctg gggataaccg agggggagaa cgtcatccac ctgcgcaccc
     4441 tgcgtcgggt caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc
     4501 tctggccgac gctgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa
     4561 cggggattac gctacgacgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag
     4621 agtgccagcg tcttgaaatc ccgaatctgt cgccgctgct gtgcgtgcgc acccttaacc
     4681 accgtgacgg tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga
     4741 ttgaattcac tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt
     4801 ccgtgctggc gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca
     4861 acgccgatta tgaggtgatc cgcgccgctg aaactggcct ggtgcagatt caggcgcgaa
     4921 tgggcggcac cggcgaacgc ttttttgctg gcgacgccac gctcacccgc gccgccgtgc
     4981 gtctgactga cggcacgctc ggctatagct gggtgctggg gcgcgacaaa cagcacgccg
     5041 aacgttgtgc gctgattgat gcgctgatgc agcaaaaccg ccactttcaa aacttatcag
     5101 aaacccttat taccccgctg gacgctgacc gtatggcacg cattgccgcg cgtcaggccg
     5161 aagtgaacgc cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct
     5221 ggaaaccgct tttatgcttc ccgtgtagga tgcccagcac agttttcgtc gcctgttaaa
     5281 ggccatgagc gagccgggcg tgattgtcgc cctgcatcag ctcaagcgcg gctggcagcc
     5341 gctgaatatc gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg
     5401 gcttgccgca ccattgagca acgatatcgt caatcagagc ctgcgttttc ataccaacgc
     5461 gccgctggtt aatcagccga aacaggcgac cttcgcggta acagatgagg cgatctccag
     5521 cgagcagctc aacgcccttt ccaccggcac tgccgttgca ccagaagcgg gcgcaacgct
     5581 gattttacag gtcaccagcc tgagcggtgg gcgtatgttg cgcctcaccg gcgcaggtat
     5641 tgccgaagaa cggatgatcg ccccgcaact gccaaagtgc attcttcatg aacttaccga
     5701 gcgcccgcac ccgttcccgc tcggcatcga cctgatcctg acctgcggcg agcgcctgct
     5761 ggctattccg cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc
     5821 gaaaaggcga tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgatactgat
     5881 ttgcctgaac tgagcgtcgc ccagattgaa cagcagctta acctcgcggt agatcgcgtg
     5941 atgaccgaag gcggtattgc cgaccgcgag ctggccgcgc tggcgctgaa acaggccagc
     6001 ggcgataacg tcgaagcgat ttttctgttg cgcgcctacc gcaccacgct ggcgaagctg
     6061 gcggtcagtg agccgctcga caccaccggg atgcgtctcg aacggcgtat ttcagcaatt
     6121 tataaagaca ttcccggcgg ccaactgctt ggccccacct acgactacac ccatcgcctg
     6181 ctcgatttca ccctgctggc aaacggcgaa acgccaacgc tgaccaccgc tgacagcgaa
     6241 cagcaaccgt cgccacacgt cttcagcctg ctggcgcgtc aggggctggc gaagtttgaa
     6301 gaagatagcg gcgcacaacc ggacgacatc acccgcacgc cgccggttta tccctgttcg
     6361 cgttcttccc gtctacaaca attaatgcgc ggtgacgaag gctatttgtt ggcgctggcc
     6421 tactccaccc aacgcggtta cgggcgcaat cacccgttcg caggcgaaat tcgcagtggc
     6481 tatatcgata tatttattgt gccggaagag ctgggatttg cggtgaacgt cggcgaattg
     6541 ttgatgaccg agtgtgaaat ggtcaacggt tttatcgacc cgccggatga accaccgcat
     6601 tttacacgcg gctatgggct ggtgttcggc atgagcgagc gcaaagcaat ggcgatggcg
     6661 ctggttgacc gtgcgttgca ggcaccggaa tacggcgagc acgcaacagg tccggcacag
     6721 gatgaagagt tcgtgctggc ccatgccgac aacgtcgaag ccgcaggctt tgtttcgcat
     6781 ctcaaactcc cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa
     6841 caggagcaga accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac
     6901 caaacgcatg atccgccgcg ccatcttaaa agcggtggca atccccggtt atcaggtgcc
     6961 gtttggcgga cgcgagatgc cgatgcccta cggctgggga accggcggca ttcagctcac
     7021 cgccagcgtg attggcgaaa gcgacgtgtt gaaggtgatt gaccagggcg cagacgacac
     7081 caccaacgcc gtgtcgattc gtcacttctt taagcgcgtg accggggtga acaccaccga
     7141 gcgcacggac gatgcgacac ttatccagac gcgtcaccgc atacccgaaa cgccgctgac
     7201 cgaagatcag atagttatct tccaggtgcc gatccccgaa ccgctgcgct ttattgagcc
     7261 gcgtgagacg gaaacgcgca ccatgcacgc gctggaggag tacggcgtga tgcaggtgaa
     7321 actgtatgaa gatatcgccc gcttcggtca tatcgccacc acctacgcct atccggtgaa
     7381 ggtgaacggg cgctacgtaa tggacccgtc gccgatcccg aaattcgata acccgaaaat
     7441 ggacatgatg cccgccctgc aactgtttgg cgcggggcgc gagaagcgca tctatgccgt
     7501 gccaccgttt acccgcgtgg aaagcctcga tttcgatgat catccgttca ccgtccagca
     7561 gtgggatgaa ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct
     7621 ggatgacgcc ggaaaacgca tgtttgtctg ctccgatacc gattattgcc gccaacagaa
     7681 cgaggccaaa agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttat
     7741 gccccgggca aaggcttcag cgatgtctct tttgatttat ggccaggaga agttctgggc
     7801 attgttgggg agtccggttc cgggaagacc acgctactga aatcaatttc cgcccgcctg
     7861 acaccacagc aaggggaaat tcgctacgag aaccgttcgc tgtacgcgat gagcgaggcc
     7921 gaccgccgtc gcctgctgcg caccgaatgg ggtgtggtgc atcagcatcc gcttgacggc
     7981 ctgcgccgcc aggtgtcggc aggcggcaat atcggcgaac ggttgatggc aaccggggcg
     8041 cgtcattacg gcgatattcg tgccaccgcg cagaagtggc tggaagaggt ggagattccc
     8101 gccaaccgga tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag
     8161 attgcccgca acctggtgac gcatccgaag ctggtgttta tggacgagcc aaccggcggg
     8221 ctggatgtat cggtacaggc gcgcctgctc gatctgctgc gcggtctggt ggtggagctg
     8281 aacctggcgg tagtgattgt tacccacgat ctgggcgtcg cccgcctgct ggcggaccgt
     8341 ttgctggtga tgaaacaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac
     8401 gaccctcacc atccatatac ccagttgctg gtgtcatcgg ttttgcagaa ttgagctgat
     8461 gccggatgcg gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa
     8521 gacgcatcag cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa
     8581 acgtcagtaa aaccttcatc ctgcaccagc aaaacggcgt gcgcctgcct gtattacaag
     8641 gcgcctcact caccgtcaac gccggcgaat gcgtggtact ccacggccat tccggcagtg
     8701 gaaaatcaac tctgctacgc tcgctgtatg ccaactatct acccgacgaa gggcaaatcc
     8761 agatcaaaca cggtgatgaa tgggtcgacc tggtcaccgc gccagcgcgc aaagtggtgg
     8821 agatccgcaa aaccactgtc ggctgggtaa gccagtttct tcgcgtcatc cctcgtattt
     8881 cggcactgga agtggtgatg cagccgctgc tcaataccgg cgtaccgcgt gaagcctgcg
     8941 ccgctaaagc cgcccgatta ctgacgcgcc tgaacgtgcc tgaacgcctg tggcaccttg
     9001 cgccatcaac cttttccggc ggcgaacagc agcgtgtcaa catcgcccgt ggctttatcg
     9061 tcgactaccc cattctgctg ctcgacgaac ctaccgcctc gctggacgct aaaaacagcg
     9121 ccgccgtagt ggaactgatc cgcgaagcca aagcccgcgg cgcggccatc gtcggtatct
     9181 tccatgacga aaccgtgcgc gagcaagtcg ccgaccgcct gcacccaatg ggagtctctt
     9241 catgattatc aataacgtca aactggtgct ggaaaacgag gtggtacacg gttctctgga
     9301 gatgcaggac ggcgaaatcc gcgcctttgc cgaaagccag agtcacctgt cagaagcaat
     9361 ggacggcgaa ggcggctggc tgctaccggg gctgattgag ctgcataccg ataatctgga
     9421 taaattcttc accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca
     9481 cgacgcgctg atggtggcga gcggcatcac caccgtgctg gacgccgtgg cgattggtga
     9541 tgtacgcgac ggcggcgatc ggctggagaa tctggagaag atgatcaacg ccatcgaaga
     9601 gacgcagaaa cgcggcgtca accgtgccga gcaccgtctg cacctacgct gcgaactgcc
     9661 gcatcacacc acgctgccgc tgtttgaaaa actggtacag cgcgaaccgg taacgctggt
     9721 gtcgctgatg gatcactcac cgggccagcg ccagttcgcc aaccgcgaaa aatatcgtga
     9781 atattatcag ggcaaatact cgctcaccga tgcgcagatg caacagtacg aagaggagca
     9841 actggcgctc gccgcacgct ggtcacagcc gaatcgcgaa actatcgccg caatgtgccg
     9901 cgcgcgacac attgccctcg ccagccacga tgacgccacg cacgcccacg ttgccgaatc
     9961 ccaccagctt ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cagaagcctc
    10021 gcgcaagcat ggcatgaacg tgctgatggg cgcgccgaat atcgtgcgcg gcggttcgca
    10081 ctccggcaac gtggcggcca gtgaactggc acagcttggc ctgctggata tcctctcttc
    10141 cgactactac cccgccagcc tgctggatgc ggcgtttcgc gtggccgatg accagagcaa
    10201 ccgctttacg ctgccgcagg cggtgaggct ggtgaccaaa aacccggccc aggcactgaa
    10261 tcttcaggat cgcggggtga ttggcgaggg caaacgcgcc gacctggtgc tggcgcatcg
    10321 caagggcaat cacattcata ttgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg
    10381 acgggaaaac tgatttggtt aatgggggcc tctggctccg gaaaagacag tctgttgacg
    10441 gaactccgcc agcgggaaca aactcagcta ctggtagcgc atcgctacat cacgcgcgcc
    10501 gccagcgccg gaagtgaaaa ccatatcgcc ctgagcgagc aggagttttt tacccgcgcg
    10561 gggcagaacc ttctggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc
    10621 gagattgacc tctggctgca cgctggattt gacgtggtgg tcaacggctc acgcgcccat
    10681 ctgccgcagg cgcgggcgcg ctatcaatcg gcgctgctgc ccgtttgttt acaggtttcg
    10741 ccagagatcc tgcgccagcg cctggaaaac cgtggtcgtg aaaatgccag tgaaattaac
    10801 gcccggctgg cgcgcgctgc ccgctatact cctcaggatt gtcttacgct caataatgac
    10861 ggcagcctgc gccagtcggt cgacacgctg ctgtcgctga ttcatcagaa ggagaaacac
    10921 catgcctgct tgtgaacttc gcccggcaac gcagtacgac accgacgcgg tttacgcgct
    10981 gatttgtgag ctaaaacaag cggagtttga ccaccaggcg tttcgcgtgg gctttaacgc
    11041 caacctgcgt gacccgaata tgcgctacca tctggcgctg ttggatggcg aggttgtcgg
    11101 catgatcggc ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca
    11161 ggaactggtg gtgatgccgc aggcgcgcgg tctgaacgtc ggcagtaaat tactggcatg
    11221 ggcagaagaa gaagcccgcc aggctggggc cgaaatgacc gaactttcca ccaacgtgaa
    11281 gcgccacgac gcgcaccgtt tctatctgcg cgaaggctac gaacagagcc atttccgctt
    11341 cacgaaggcg ctgtaatatg agcctgacct tcacgctcac cggcaccggc ggcgcacagg
    11401 gcgttccggc atggggctgt gattgcgtgg cctgcgccag agcgcgacgc tcaccgcagt
    11461 atcgccgcca gccatgcagc ggcgtagtga agtttaacga ggcgatcacc ctgatcgacg
    11521 ccgggcttca cgatctcgcc gatcgctggt ctcccggatc gttccagcag tttttactga
    11581 cgcactacca tatggatcac gtccaggggc tgttcccgct gcgctggggc gtgggcgaca
    11641 caatcccggt ctacggtcca ccggatgagc agggctgcga cgatctgttt aaacatccgg
    11701 gcctgcttga tttcagccac acggtggagc cgtttgtggt gtttgatttg caggggttgc
    11761 aggtcacgcc gctgccgctt aaccattcga aactcacttt cggctatcta ctggaaacgg
    11821 cacatagccg ggtggcgtgg ctgtctgaca ctgcaggcct gccggaaaaa acgctgaaat
    11881 ttttacgcaa taatcagcca caggtaatgg tgattgattg cagccacccg ccgcgcgagg
    11941 atgcaccgcg taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct
    12001 cgccgcgggt gattctgacc catatcagcc atcagtttga tgcgtggctg atggaaaacg
    12061 cactgccgtc ggggtttgag gcgggatttg atgggatgga gattgaggtg gagtgaggga
    12121 aaatacccgc gccggcccct acctcaacgc ccatactcat cttccattct ccgctcttca
    12181 tcttccagtt gccgacgctc ctgatcaagc tggcgctggc gatcgtccag ctgcctgcgg
    12241 cgatcttcaa actggcggcg gcggtcatca tattgtctgc gccgatcgtc gctcacttca
    12301 cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc cacggtcagg gttataagcg
    12361 tcgttaatcg cctgctgaat attgccaatg gtgtcgtcga taatatcggc ctgggccgga
    12421 acggtggaca gcgtgagcag ggtgaataaa agaaatagcg gaaagcgttt cattagccaa
    12481 cctcaaaaag aaactctatc cacattagtc attactcatc catgcaagta gtggatgaat
    12541 ctcaattcct ttgctgctct attgccgtaa tcgcctccac gcgttgttga tgacgaccgc
    12601 cttcgtactg tgcgcccagc cacgcatcga caatcatttt tgccagttcg aggccaacca
    12661 ctcgtgaacc aaaagccagc acgttggtgt cgttatgctg ccgcgaaagt tgcgcggaat
    12721 aaggttcgct acaaacgacc gcgcgaattc cggcaaactt gttcgccgct atcgaaatac
    12781 cgacgccagt gccacaaatc aaaatcccgc catcagcctc tccgccagca acggccagtg
    12841 cgacttggct ggcgtaatgc ggataatcag tacgctctga cgaccaggtt cctttatcaa
    12901 tcacttcaac gtcacgctca actaaatgtg ccactatttc atgttttaaa atgaaaccga
    12961 catgatcaca gccaaatgca atctttttca ttcatcttcc ccgcccgcgc aaaacttcac
    13021 aatcaaaaaa catttgtgat gtaaatgaat taaaaacacc caaaatcgat gaaatccgcc
    13081 ttcccgatct gttttacaga gttacatctg gcagcgcaat agcgttaaag acactcacta
    13141 tccagcaaca tctatcatct aataaaccag gaaaacaaat aacatcatgt ttttaaagtg
    13201 attaaatgaa acgaaatttt aagccactct ccattattca caacaaaaca aactttataa
    13261 attttatttt tttgtgaagt tgccagcatc ttttctgttc ttgctgtggt gatatagtgg
    13321 cgtcttcaat tcaaggacaa gagaacgtga tgagccagtc agagtttgat tcagcgcttc
    13381 cgaacggtat cgggttagcg ccttacctgc gaatgaagca ggaaggaatg acagaaaacg
    13441 aaagccgcat cgtggagtgg ttactcaaac ccggtaacct gagttgtgca cccgcaatta
    13501 aagatgtcgc agaagctctg gcggtatctg aagcgatgat cgttaaggta tcaaagctgc
    13561 tggggtttag cggctttcgt aacttacgca gtgcgctgga agattatttt tctcagtcag
    13621 aacaagtatt gccttccgag ttggcttttg atgaagcgcc gcaggatgtg gtgaataagg
    13681 tatttaacat cactttacgc accattatgg aaggtcagtc gatcgtcaac gttgatgaga
    13741 tccaccgtgc cgcccgcttt ttctatcagg ccagacagcg ggatttgtac ggtgccggag
    13801 gatcaaatgc tatctgtgct gatgtacagc acaagttttt gcgcattggc gtacgctgcc
    13861 aggcctatcc ggacgctcac atcatgatga tgtctgcttc gttgttacag gaaggagatg
    13921 tggtgctggt ggtgactcat tccgggcgaa ccagtgatgt aaaagcggct gtagaactgg
    13981 caaaaaagaa cggggcaaag attatttgta taacgcatag ctatcattca ccaatagcga
    14041 agctggccga ttatattatt tgctcaccgg ccccggaaac accgttatta ggtcgtaatg
    14101 cctcggcaag aatattacaa ctgactttgc tggacgcgtt ttttgtctct gtcgcccagc
    14161 tcaatattga acaagctaat attaatatgc aaaaaaccgg cgcaattgtt gatttcttct
    14221 caccaggcgc gctgaaataa gaaaaaggta attccgccat cgcggagtta cctctcccct
    14281 taccgtgaga acatgattat gaataaatat ctgaaatatt tcagcggcac actcgtgggc
    14341 ttaatgttgt caaccagcgc ttttgctgcc gccgaatatg ccgtcgtatt gaaaacactc
    14401 tccaatccat tttgggtaga tatgaaaaaa ggcattgaag atgaagcaaa aacgctgggc
    14461 gtcagcgttg atatttttgc ctctccttca gaaggcgatt ttcaatctca attgcagtta
    14521 tttgaagatc tcagtaataa aaattacaaa ggtatcgcct tcgcaccatt atcctcagtg
    14581 aacctggtca tgcctgtcgc ccgcgcatgg aaaaaaggca tttatctggt caatctcgat
    14641 gaaaaaatcg acatggataa tctgaaaaaa gcaggcggta atgtggaagg ttttgtcacc
    14701 accgataatg ttgccgtcgg ggcgaaaggc gcgtcattca ttattgacaa gctgggtgcc
    14761 gaagggggtg aagtcgcaat cattgagggt aaagccggta acgcctccgg tgaagcgcgt
    14821 cgtaatggtg ccaccgaagc cttcaaaaaa gcaagccaga tcaagcttgt cgccagccag
    14881 cctgccgact gggaccgtat taaagcactg gatgtcgcca ctaacgtgtt gcaacgtaat
    14941 ccgaatatta aagcgatcta ttgcgcgaat gacacgatgg caatgggtgt tgctcaggct
    15001 gtcgcaaacg ccagaaaaac gggaaaagtg ctggtcgtcg gtacagatgg cattccggaa
    15061 gcccgcaaaa tggtggaagc cggacaaatg accgcgacgg ttgcccagaa cccggcggat
    15121 atcggtgcaa cgggtctgaa gctgatggtt gacgctgaga aatccggcaa ggttatcccg
    15181 ctggataaag caccggaatt taaactggtc gattcaatcc tggtcactca ataaaactga
    15241 atgatgccgg atgatgatgc taataaagag tcttatccgg ccaacaaaat aacctgaacg
    15301 tagacctgat aagtgtagcc catcaggcaa taatacgata tttcgtgcag agaggttatt
    15361 tatggccacg ccatatatat cgatggcggg gatcggcaag tcctttggtc cggttcacgc
    15421 attaaagtcg gttaatttaa cggtttatcc tggtgaaata catgcattac taggagaaaa
    15481 tggcgcgggt aaatccacgc taatgaaagt gttatccggg atacatgagc cgaccaaagg
    15541 caccattacc attaataaca ttaactataa caaactggat cataaattag cggcacaact
    15601 cggtatcggg attatttatc aggaactcag cgttattgat gaattaaccg tactggaaaa
    15661 tttatatatt ggtcgtcatc tgacgaaaaa aatctgtggc gtcaatatta tcgactggcg
    15721 agaaatgcgt gtccgcgccg ccatgatgtt attacgcgtg ggcttgaaag ttgatttaga
    15781 tgagaaagtg gcgaacttat ctatcagcca caagcagatg ctggaaattg ccaaaacgct
    15841 gatgctcgat gccaaagtca tcatcatgga tgaacccacc tcctcactca ccaataaaga
    15901 ggtggactat ctgtttctga tcatgaatca gttgcgtaag gagggtacgg ccatcgtcta
    15961 tatctcgcat aaattggcgg aaattcgccg tatttgcgac cgctatacgg tgatgaaaga
    16021 cggcagcagc gtttgcagcg gcatggtgag cgatgtgtca aatgacgata tcgtccgtct
    16081 gatggtaggc cgcgaattgc aaaaccgttt taacgcgatg aaggagaatg tcagcaacct
    16141 tgcgcacgat acggtttttg aggtgcgcaa cgtcaccagc cgtgacagaa aaaaggtccg
    16201 ggatatctca tttagcgtct gccggggaga aatattaggc tttgccgggc tggtcggttc
    16261 cggacgtaca gagctgatga actgcctgtt tggcgttgat aaacgcgctg gcggagaaat
    16321 ccgtcttaat ggcaaagata tctctccacg ttctcccctg gatgccgtga aaaaagggat
    16381 ggcttacatc actgaaagcc gccgggataa cggctttttc cccaactttt ctatcgctca
    16441 gaacatggct atcagccgca gtctgaaaga cggcggctat aaaggcgcga tggggttgtt
    16501 tcatgaagtt gacgagcaac gtaccgctga aaatcaacgc gaactgctgg cgctgaaatg
    16561 tcattcggta aaccagaaca tcactgaact ctccggcggt aaccagcaga aagtcctgat
    16621 ctccaaatgg ctgtgctgtc gcccggaagt gatcattttc gatgaaccta cccgtggcat
    16681 cgatgttggc gcgaaagccg aaatttacaa agtgatgcgc caactggcgg acgacggaaa
    16741 agtcatcctg atggtgtcat ctgaactacc tgaaattatc accgtctgcg accgcatcgc
    16801 cgtgttctgc gaaggacgac tgacgcaaat cctgaccaat cgcgatgaca tgagcgaaga
    16861 ggagattatg gcatgggctt taccacaaga gtaaaaagcg aagcgagcga gaagaaaccg
    16921 ttcaactttg cgctgttctg ggataaatac ggcacctttt ttatcctggc gatcatcgtc
    16981 gccatttttg gttcgctgtc gccagaatat tttctgacca ccaataatat tacccagatt
    17041 tttgtgcaaa gctccgtgac ggtactgatc ggcatggggg aattcttcgc tatcctggtc
    17101 gccggtatcg acctttcggt tggcgcgatt ctggcgcttt ccggtatggt gaccgctaaa
    17161 ctgatgttgg caggtattga tccgtttctc gcggcgctaa ttggcggtgt actggttggc
    17221 ggcgcactgg gggcgatcaa cggctgcctg gtcaactgga cgggactaca cccgttcatt
    17281 atcacccttg gcaccaacgc tattttccgt gggatcacgc tggtgatctc cgatgccaac
    17341 tcggtatacg gcttctcatt tgacttcgtg aacttctttg ccgccagcgt aattgggata
    17401 cctgtccccg ttatcttctc gctaattgtc gcgctcatcc tttggtttct gacaacgcgt
    17461 atgcggctcg gacgcaacat ctacgcactg ggcggcaaca aaaactcggc gttctattcc
    17521 gggattgacg tgaaattcca catcctggtg gtgtttatca tctccggtgt ttgtgcaggt
    17581 ctggcaggcg tcgtctcaac tgcacgactc ggtgccgcag aaccgcttgc cggtatgggt
    17641 tttgaaacct atgccattgc cagcgccatc attggcggca ccagtttctt cggcggcaag
    17701 gggcgcattt tctctgtggt gattggcggg ttgatcatcg gcaccatcaa caacggtctg
    17761 aatattttgc aggtacaaac ctattaccaa ctggtggtga tgggcggatt aattatcgcg
    17821 gctgtcgccc ttgaccgtct tatcagtaag taaggaattg aacatgaaaa tctccccctc
    17881 gttaatgtgt atggatctgc tgaaatttaa agaacagatc gaatttatcg acagccatgc
    17941 cgattacttt cacatcgata tcatggacgg ccactttgtg ccgaatctga cgctctcacc
    18001 gttcttcgta agccaggtaa aaaaactggc gagtaaaccg ctcgactgcc atctgatggt
    18061 aacgcgtccg caggattaca ttgctcaact ggcacgtgcg ggagctgatt tcatcacttt
    18121 gcatccggaa actatcaacg gtcaggcatt ccgcctgatt gatgaaatcc gccgtcatgg
    18181 catgaaagtg gggctgattc ttaatccgga aacgccggtt gaggccatga aatactatat
    18241 ccataaggct gataaaatta cggtcatgac tgtcgatccc ggttttgccg gacaaccctt
    18301 cattcctgaa atgctggata aagttgccga actgaaggca tggcgtgaac gagaaggtct
    18361 ggagtacgaa attgaggtgg acggttcctg caaccaggca acttacgaaa aactaatggc
    18421 ggcaggggcg gatgtcttta tcgtcggcac ttccggcctg tttaatcatg cggaaaatat
    18481 cgacgaagca tggagaatta tgactgcgca gattctggct gcaaaaagcg aggtacagcc
    18541 tcatgcaaaa acagcataac gtcgtagcgg gcgtggatat gggggcaacg catatccgct
    18601 tttgtctgcg gacagcagaa ggtgaaacgc tatactgcga aaaaaagcgg accgcagaag
    18661 ttattgctcc cgacctggtg tcgggtatcg gcgaaatgat tgacgagcaa ctcaggcgct
    18721 ttaacgctcg ctgtcatggt ctggtgatgg gatttccggc gctggtcagt aaagataaac
    18781 gcaccattat ttctacgcct aacctgccgt taacagcggc agatttatat gatctcgccg
    18841 ataagctcga aaatacgctg aattgtccgg ttgagttttc ccgcgacgtt aacctgcaac
    18901 tctcctggga cgtagtagaa aaccgcctta cgcaacaact ggttctggca gcctatctcg
    18961 gtacggggat ggggttcgca gtgtggatga acggtgcgcc gtggacgggt gcacacggtg
    19021 tggcaggcga actgggccat atccccctgg gagatatgac ccaacactgc gcgtgtggca
    19081 attctgggtg cctggaaacc aattgctctg gaatggcgct aagacgctgg tacgaacaac
    19141 agccccgaaa ttacccattg agcgatcttt tcgtccatgc ggaaaacgcc cctttcgtcc
    19201 agagtctgct tgaaaacgcg gcacgagcca ttgccaccag cattaatctg ttcgatcccg
    19261 atgcggtgat tctgggtggt ggcgtgatgg atatgcccgc cttcccacgc gagactctca
    19321 ttgccatgac ccaaaagtac ctgcgccgtc cactgccgta tcaggtcgtg cgctttattg
    19381 ccgcctcatc ttctgacttt aatggcgctc agggtgcagc aatattggcg catcaacgtt
    19441 ttttgctaca gtcctgtgct aaagtcccat gagtaaagcg ttggcacagt agcgcaatat
    19501 cccgctgtgc caacgacagg tacaatatat ataataatta cataaaacag cccatattat
    19561 tcccagcaat gatagttcta ctgtaatttt ccctcttcag cacaaatgcc taataaaatg
    19621 gctcgttccg ctcacacttc cacttattaa agtcgcaata tcagaattat gataacgcca
    19681 gctttaaatg acgtattttg catggattac atatttacga cataaatgat aaatggagat
    19741 ttatatgaat aactctcggt tattccgttt gagcaggatt gttattgcgt tcactgccgc
    19801 cagcagcatg atgataaata ccgcttacgc aacagatgaa gtgaaagccg ccactcaata
    19861 tacccaacag gttaatcaga attacgccaa atcattaccg tttagcgatc gtcaggattt
    19921 tgacgatgct cagcgtggat ttatcgctcc gctgctggat gaaggtattc tgcgtgatgc
    19981 gaacggtaaa atttactacc gcgcgaatga ttacaaattt gatattaatg ccgctgcgcc
    20041 ggaaaccgta aaccccagcc tgtggcgtca gtcgcagatc aacggcattt caggcctgtt
    20101 caaagtcacc gataaaatgt atcaggtgcg cggccaggat atctctaaca ttacgttcgt
    20161 tgagggcgag aaaggcatta ttgttattga cccactggtg acgccacctg ccgcaaaagc
    20221 cgcacttgac ctttacttcc agcatcgccc gcaaaaaccc attattgccg ttatctatac
    20281 ccacagccac gccgaccact atggcggcgt gaaaggcatc atctctgaag ccgatgttaa
    20341 atccggcaaa gttcaggtga ttgctccagc tggctttatg gacgaagcca tcagcgaaaa
    20401 cgtgctggcg ggtaacatca tgagccgccg tgcgctctac tcttacggtc tgttactgcc
    20461 gcacaacgcg caaggcaatg tgggtaatgg ccttggcgtg acgctggcaa cgggcgaccc
    20521 gagcattatt gcaccgacta aaactatcgt cagaactggc gagaagatga ttatcgacgg
    20581 cctggagttt gacttcctga tgaccccagg tagcgaagcg ccagccgaaa tgcacttcta
    20641 tattccggcc ctgaaagccc tgtgtaccgc cgagaacgcc acgcataccc tgcacaactt
    20701 ctacactctg cgcggcgcga aaacccgcga caccagcaag tggaccgagt atctgaacga
    20761 aacgttggat atgtggggta acgacgcgga agtactgttt atgccgcaca cctggccggt
    20821 ctggggcaat aagcatatca atgattatat tggtaaatat cgcgatacta tcaagtacat
    20881 tcacgaccag accctgcacc tggcgaacca gggctacacc atgaatgaaa tcggcgacat
    20941 gattaagctg ccgcctgcgc ttgccaataa ctgggccagc cgcggttatt acggttctgt
    21001 cagccacaac gcccgcgcgg tgtataactt ctatcttggc tattacgacg gtaacccggc
    21061 taacctgcat ccgtatggtc aggtggagat gggtaaacgt tacgtgcagg cgctgggcgg
    21121 ttctgcccgc gtcatcaacc tggcgcaaga agcgaacaag caaggtgatt atcgctggtc
    21181 ggcagaactg ctgaaacagg tgattgccgc caacccgggt gaccaggtcg cgaagaatct
    21241 gcaagcgaat aactttgaac agctgggcta tcaggccgag tccgccacat ggcgcggttt
    21301 ctacctgacc ggcgcgaaag agctgcgcga aggggtgcat aagttcagcc acggcaccac
    21361 cggttccccg gacaccattc gtgggatgtc ggtcgaaatg ctgttcgact ttatggccgt
    21421 tcgcctcgat agcacgaaag ccgcaggtaa aaatatcagc ctgaacttca atatgagcaa
    21481 cggcgataac ctcaacctga tgctgaacga tagcgtgctt aactaccgta aaacgctgca
    21541 atcgcaagcc gacgcctctt tctacattag ccgtgaagat ctgcacgccg tgctgaccgg
    21601 acaggctaaa atggccgatc tggtgaaagc gaagaaagcc aaaattattg gcaatggcgc
    21661 gaaactggaa gaaattatcg cctgtctgga taatttcgat ttgtgggtga atatcgtaac
    21721 cccaaattaa tgtcactcgc ttttgggggc gctgaagccc ccaaagcatt tgctttaaaa
    21781 atatatacag ccaattaata aataaaatat ataagttatg aatgcggtgg ctgaataaag
    21841 ctatttttat tcatcgagaa aatagccata ctgacgcctt caatggatat tgatattcac
    21901 tacagatatt gttcgcagca gacaggatgt atttaatatg ccaacagttc tctctcgcat
    21961 ggcgatgcaa ctcaaaaaaa cagcctggat aattcccgtc ttcatggttt cgggatgctc
    22021 attatctccg gcaatcccgg tgatcggcgc ttattatccc ggctggtttt tctgcgccat
    22081 tgccagcctt attttgacgc tcatcacgag gcgaattatt cagcggacaa atatcaatct
    22141 ggcatttgtc ggaattattt ataccgccct ttttgctctc tacgccatgc tgttctggct
    22201 ggcatttttc taatctattt gagacgcgct catggaaagt acgccgaaaa aagctcctcg
    22261 cagtaaattc cctgctctgt tagtggttgc gttggcgctg gttgcccttg ttttcgttat
    22321 ctggcgcgta gacagtgcgc catcaactaa tgacgcttac gcgtcagcag ataccattga
    22381 tgtggtgccg gaagtcagcg gtcgcattgt tgaactggcg gtcaccgaca accaagcggt
    22441 gaaacagggc gatttgctgt tccgcattga cccgcgcccg tacgaagcca atctggcgaa
    22501 agctgaagcc tccctcgcgg cgctggataa gcaaattatg ctcacccagc gtagcgttga
    22561 cgcgcaacag tttggtgccg actcggttaa tgccacggta gaaaaagccc gtgccgccgc
    22621 gaaacaagcc agcgatacgt tacgccgtac cgaaccgcta ctgcgagaag gttttgtttc
    22681 tgcggaagag gttgaccgtg caagaaccgc acagcgtgcg gcagaagcgg accttaatgc
    22741 cgtattgttg caggcgcagt cagccgccag cgccgtcagc ggcgtggatg cgctggtggc
    22801 gcagcgtgcg gcagtggaag cggatattgc cctgaccaaa ctgcatctgg aaatggcgac
    22861 cgttcgcgcg ccgtttgatg gccgggtcat ttccctcaaa acctccgtcg ggcagtttgc
    22921 atctgcgatg cgccctattt tcactctaat cgacactcgt cactggtacg tgattgccaa
    22981 cttccgcgaa actgatctga aaaatattcg ctcgggtaca ctcgcaacca ttcgcctgat
    23041 gagcgacagc ggcaaaacct tcgagggtaa agtggattcg attggctacg gcgtgctacc
    23101 ggatgacggc ggcctggtgc tgggcggcct gccgaaagtg tcacggtcta ttaactgggt
    23161 ccgcgttgcc cagcgttttc cggtcaaaat catggttgat aaacctgacc cggaaatgtt
    23221 ccgcatcggc gcttcggcag tcgctaatct tgagccgcaa taatgagcgc gctcaactcc
    23281 ctgccattac cggtggtcag gctgctggcg ttctttcatg aagagttaag cgagcggcga
    23341 ccagggcgcg tgccacagac gatgcaactc tgggtaggtt gcctgctggt gattctgatc
    23401 tcgatgactt ttgagatccc ttttgtggcg ttatcactgg cagtgctgtt ttacggtatt
    23461 cagtcgaacg cgttttacac caaatttgtc gcgatcttgt ttgtggttgc cacggtgctg
    23521 gagatcggca gcctgttttt gatctacaaa tggtcatatg gcgaaccgtt gatccgattg
    23581 atcatcgccg ggccgatcct gatgggctgc atgtttttga tgcgcaccca tcggctggga
    23641 ctggtctttt tcgccgtcgc cattgtcgct atttacgggc aaaccttccc cgctatgctc
    23701 gactatccgg aagcggtcgt gcgcttaacg ctgtggtgta tcgttgttgg cctctatcca
    23761 accttgctga tgacgttaat cggcgtgctg tggtttccca gtcgtgccat tacgcagatg
    23821 catcaggcgc ttaatgatcg gcttgatgat gccattagcc acctgacgga cagcctcgca
    23881 ccgctacccg aaacgcggat tgaaagagag gcgctggcgc tgcaaaaact caatgtcttt
    23941 tgcctcgcgg acgatgccaa ctggcgaacc cagagcgcat ggtggcaaag ctgcgtggca
    24001 acggtaacct acatttactc gacgctgaat cgctacgatc ccacctcttt tgctgattct
    24061 caggcaatta ttgaattccg acaaaaatta gcttcagaaa tcaacaatct gcagcattcc
    24121 attaccgaag gtcagtgctg gcaaagcgac tggcggatca gtgaaagtga agcgatggcg
    24181 gcacgggaat gtaacctgga gaatatctgc cagacgctgt tacagcttgg tcagatggac
    24241 ccgaatacgc cgccaacgcc cgcagccaaa ccgccatcaa tggtcgccga tgcttttacc
    24301 aatccagact atatgcgcta cgcggtaaaa acgctgctcg cctgtttgat ctgttacacc
    24361 ttctacagcg gcgtggactg ggaaggcatt catacctgta tgctgacctg cgtgatcgtc
    24421 gctaatccaa atgtcggttc gtcgtaccag aagatggtgc tgcgttttgg tggggccttt
    24481 tgcggcgcga ttctggcgct gttattcacg ctactggtca tgccctggct ggacaatatt
    24541 gtcgaattgc tgtttgtgct ggcaccgatt ttcctgttgg gcgcatggat tgccaccagc
    24601 tctgaacgct cttcttatat cggcacacag atggtggtca ccttcgcgct cgccacgctc
    24661 gaaaacgttt ttggcccggt gtacgacctg gtggaaattc gcgatcgcgc tctgggtatc
    24721 atcattggta ccgtggtgtc ggcggtgatt tacacctttg tctggcctga aagcgaagcg
    24781 cgcacgctgc cgcaaaaact ggctggcgcg ctgggtatgt tgagtaaagt aatgcggatc
    24841 ccacgccagc aggaagtcac ggctctgcgc acttatctgc acattcgcat aggtctgcat
    24901 gcggcgttta atgcctgtga agagatgtgc caacgcgtgg tgctggagcg tcaactggac
    24961 agcgaagaac gcgcattact gattgaacgt tcgcaaacgg ttattcgtca gggccgcgat
    25021 attcttcacg cctgggatgc cacctggaac tcggcgcagg cgctggataa cgcactacag
    25081 ccggacagag caggtcagtt tgccgacgcc ctggagaaat acgccgccgg tgtggctacc
    25141 gcactcagtc attctcctca aataacgctt gaagaaacac ccgcctcgca ggccatcctg
    25201 ccgaccttat taaaacagga gcaacacgtc tgccagcttt tcgcccgctt gccagactgg
    25261 acagccccgg cattaacgcc cgccacggaa caggcacaag gagccacgca atgatcaatc
    25321 gtcaactttc acgcctgctg ttgtgcagca ttctcggcag cacgacgctg atttccggct
    25381 gtgccctggt acgtaaggat tctgcgcctc atcaacagct caaaccggaa caaatcaaac
    25441 tggccgatga tattcatctt gccagctccg gctggccgca ggcgcagtgg tggaaacaac
    25501 tcaatgaccc gcagctggat gcgctgatcc aacggacgct aagtggttca caccccctcg
    25561 ccgaagcgaa actgcgggaa gaaaaagcgc agtcccaggc cgatttgtta gatgccggtt
    25621 cacaattaca ggtcgcagcg ttagggatgc tcaaccgcca acgcgtctcg gcgaacggct
    25681 ttttaagccc ttatgcgatg gatgcgccag cactgggtat ggacgggccg tactatacgg
    25741 aagccacagt cggtttgttt gccggactgg atctcgattt gtggggtgtg catcgctcag
    25801 cggttgctgc cgccattggc gcgcataatg ccgcgctggc agaaaccgca gcagtagagc
    25861 tatcgctgac cacgggcgta gcgcagcttt attacagtat gcaggccagc tatcagatgc
    25921 tcgatctgtt agaacaaact cgcgatgtga ttgattacgc ggtgaaagcg caccagagta
    25981 aagtggcgca cggtctggaa gcgcaagtgc ctttccacgg cgcgcgggca cagattctgg
    26041 cggtcgataa acaaattgct gccgtcaaag ggcaaatcac tgaaacgcga gaatctctgc
    26101 gtgcattgat tggcgcggga gccagcgata tgccggagat caaaccggtg gcattaccac
    26161 gagtacagac cggcattccg gcaacgctct cttatgagtt gctcgccaga cgcccggatc
    26221 tgcaagccat gcgctggtat gttcaggcgt cattagatca ggtggattcc gcgcgggcac
    26281 tgttctaccc gagctttgat atcaaagcgt ttttcggtct ggactccatc cacctggata
    26341 ccttattcaa aaaaaccagt cgccagttca acttcatccc gggtctgaaa ttgccgctgt
    26401 ttgacggtgg acggttgaat gccaatctcg aaggcactcg cgccgccagc aacatgatga
    26461 ttgaacgtta caaccagtca gtactgaacg cggtgcgtga cgttgccgtc aacggcacgc
    26521 gtctgcaaac gctcaacgac gagcgagaga tgcaggctga acgcgtagaa gcaacgcgct
    26581 tcacccagcg cgcagccgag gccgcctatc agcgcggctt aaccagccgc ttacaggcca
    26641 ccgaagcccg gttgccagtg cttgccgagg agatgtcatt actgatgctg gacagccgcc
    26701 gggtgatcca aagcattcag ttgatgaaat cgctgggcgg cgggtatcag gcggctcccg
    26761 tcgtcgagaa aaaataaaat gtctaccgcg tgatggctgt cacgcggtat ttcgtttcgt
    26821 cacgtcaaaa ctgacgacag cctgtttttc gtcagagttt tgaataaata gtgcccgtaa
    26881 tatcagggaa tgaccccaaa taaaatgtgg cataaaagat gcatactgta gtcgagagcg
    26941 cgtatgcgtg atttgattaa ctggagcgag accgatgaaa aaagtcgtca cggtttgccc
    27001 ctattgcgca tcaggttgca aaatcaacct ggtcgtcgat aacggcaaaa tcgtccgggc
    27061 ggaggcagcg caggggaaaa ccaaccaggg taccctgtgt ctgaagggtt attatggctg
    27121 ggacttcatt aacgataccc agatcctgac cccgcgcctg aaaaccccca tgatccgtcg
    27181 ccagcgtggc ggcaaactcg aacctgtttc ctgggatgag gcactgaatt acgttgccga
    27241 gcgcctgagc gccatcaaag agaagtacgg tccggatgcc atccagacga ccggctcctc
    27301 gcgcggtacg ggtaacgaaa ccaactatgt aatgcaaaaa tttgcgcgcg ccgttattgg
    27361 taccaataac gttgactgct gcgctcgtgt ctgacacggc ccatcggttg caggtctgca
    27421 ccaatcggtc ggtaatggcg caatgagcaa tgctattaac gaaattgata ataccgattt
    27481 agtgttcgtt ttcgggtata acccggcgga ttcccaccca atcgtggcga atcacgtaat
    27541 taacgctaaa cgtaacgggg cgaaaattat cgtctgcgat ccgcgtaaaa ttgaaaccgc
    27601 gcgcattgct gacatgcaca ttgcattgaa aaacggctcg aacatcgcgc tgttgaatgc
    27661 gatgggccat gtcattattg aagaaaatct gtacgacaaa gcgttcgtcg cttcccgtac
    27721 agaaggcttt gaagagtatc gtaaaatcgt tgaaggctac acgccggagt cggttgaaga
    27781 tatcaccggc gtcagcgcca gtgagattcg tcaggcggcg cggatgtatg cccaggcaga
    27841 aagcgccgcc atcctgtggg gcatgggggt aacccagttc tatcagggcg tggaaaccgt
    27901 gcgttctctg accagcctcg cgatgctgac cggtaacctc ggtaagccac atgcgggtgt
    27961 aaacccggtt cgtggtcaga acaacgtgca gggtgcctgc gatatgggcg cgctgccgga
    28021 tacgtatccg ggctatcagt acgtgaaaga tccggctaac cgcgagaaat tcgccaaagc
    28081 ctggggcgtg gaaagcctgc ctgctcatac cggctatcgc atcagcgagc tgccgcaccg
    28141 cgtggcgcat ggtgaagtga gagcggcgta cattatgggc gaagatccgc tgcaaactga
    28201 cgcggaactg tcggcagtac gtaaagcctt tgaagatctg gaactggtta tcgttcagga
    28261 catctttatg accaaaaccg cgtcggcggc ggatgtcatt ttaccgtcaa cgtcgtgggg
    28321 cgagcatgaa ggcgtgttta ctgcggctga ccgtggcttc cagcgtttct tcaaggcggt
    28381 tgagccgaag tgggatctga aaacggactg gcaaatcatc agtgaaattg ccactcgtat
    28441 gggttatccg atgcactaca acaacaccca ggagatctgg gacgagttgc gtcatctgtg
    28501 cccggatttc tacggtgcga cttacgagaa aatgggcgaa ctgggcttca ttcagtggcc
    28561 ttgccgcgat acttcagatg ccgatcaggg gacttcttat ctgtttaaag agaagtttga
    28621 taccccgaac ggtctggcgc agttcttcac ctgcgactgg gtagcgccaa tcgacaaact
    28681 caccgacgag tacccgatgg tactgtcaac ggtgcgtgaa gttggtcact actcttgccg
    28741 ttcgatgacc ggtaactgtg cggcactggc ggcgctggct gatgaacctg gctacgcaca
    28801 aatcaatacc gaagacgcca aacgtctggg tattgaagat gaggcattgg tttgggtgca
    28861 ctcgcgtaaa ggcaaaatta tcacccgtgc gcaggtcagc gatcgtccga acaaaggggc
    28921 gatttacatg acctaccagt ggtggattgg tgcctgtaac gagctggtga ccgaaaactt
    28981 aagcccgatt acgaaaacgc cggaatacaa atactgcgcc gtgcgcgtcg agccgatcgc
    29041 cgatcagcgc gccgccgagc agtacgtgat tgacgagtac aacaagttga aaactcgcct
    29101 gcgcgaagcg gcactggcgt aataccgctt aaactcacgt taaaagcggc gatattaccc
    29161 aacgcctttg tatcaatccg gaaacgactc tctgtttccg gattttttta tacatacctg
    29221 cgcagtcaaa ccagcccatc ctggttatta gcgacgtctt ctcggttgtt cttctattcc
    29281 agtcacgatt gcaaatgaga attatcttta ctcaattctc atcgcggcct aacgtaataa
    29341 tactttacat ttgataaaca aaatttcggt aaggtgagaa atcatcgacc gtttaactcg
    29401 ttctattaac gggatatttt ggcgcgatta at
//
LOCUS       DAZKHK010000061        29260 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKHK010000061 DAZKHK010000000
VERSION     DAZKHK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.061"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(109..696)
                     /gene="mog"
                     /locus_tag="ACHOS5_002682"
     CDS             complement(109..696)
                     /gene="mog"
                     /locus_tag="ACHOS5_002682"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP9777474.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAAEVVAAFRPKSARREVSE"
     gene            complement(811..1764)
                     /gene="tal"
                     /locus_tag="ACHOS5_002683"
     CDS             complement(811..1764)
                     /gene="tal"
                     /locus_tag="ACHOS5_002683"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9777475.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            2043..3473
                     /gene="yaaJ"
                     /locus_tag="ACHOS5_002684"
     CDS             2043..3473
                     /gene="yaaJ"
                     /locus_tag="ACHOS5_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP9777476.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYAIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSFPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            3543..4319
                     /gene="yaaA"
                     /locus_tag="ACHOS5_002685"
     CDS             3543..4319
                     /gene="yaaA"
                     /locus_tag="ACHOS5_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP9777477.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(4472..4768)
                     /gene="yaaX"
                     /locus_tag="ACHOS5_002686"
     CDS             complement(4472..4768)
                     /gene="yaaX"
                     /locus_tag="ACHOS5_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP9777478.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPSPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(4982..6268)
                     /gene="thrC"
                     /locus_tag="ACHOS5_002687"
     CDS             complement(4982..6268)
                     /gene="thrC"
                     /locus_tag="ACHOS5_002687"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP9777479.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(6269..7201)
                     /gene="thrB"
                     /locus_tag="ACHOS5_002688"
     CDS             complement(6269..7201)
                     /gene="thrB"
                     /locus_tag="ACHOS5_002688"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP9777480.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(7203..9665)
                     /gene="thrA"
                     /locus_tag="ACHOS5_002689"
     CDS             complement(7203..9665)
                     /gene="thrA"
                     /locus_tag="ACHOS5_002689"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP9777481.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(9691..9810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(9746..9811)
                     /gene="thrL"
                     /locus_tag="ACHOS5_002690"
     CDS             complement(9746..9811)
                     /gene="thrL"
                     /locus_tag="ACHOS5_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP9777482.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(10025..10711)
                     /gene="yjtD"
                     /locus_tag="ACHOS5_002691"
     CDS             complement(10025..10711)
                     /gene="yjtD"
                     /locus_tag="ACHOS5_002691"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9777483.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(11111..11251)
                     /gene="yjjY"
                     /locus_tag="ACHOS5_002692"
     CDS             complement(11111..11251)
                     /gene="yjjY"
                     /locus_tag="ACHOS5_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP9777484.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            11347..12063
                     /gene="arcA"
                     /locus_tag="ACHOS5_002693"
     CDS             11347..12063
                     /gene="arcA"
                     /locus_tag="ACHOS5_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP9777485.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLISPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(12123..13475)
                     /gene="creD"
                     /locus_tag="ACHOS5_002694"
     CDS             complement(12123..13475)
                     /gene="creD"
                     /locus_tag="ACHOS5_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP9777486.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRYS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(13533..14957)
                     /gene="creC"
                     /locus_tag="ACHOS5_002695"
     CDS             complement(13533..14957)
                     /gene="creC"
                     /locus_tag="ACHOS5_002695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP9777487.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVASEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(14957..15646)
                     /gene="creB"
                     /locus_tag="ACHOS5_002696"
     CDS             complement(14957..15646)
                     /gene="creB"
                     /locus_tag="ACHOS5_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP9777488.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(15659..16132)
                     /gene="creA"
                     /locus_tag="ACHOS5_002697"
     CDS             complement(15659..16132)
                     /gene="creA"
                     /locus_tag="ACHOS5_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP9777489.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            16343..17212
                     /gene="robA"
                     /locus_tag="ACHOS5_002698"
     CDS             16343..17212
                     /gene="robA"
                     /locus_tag="ACHOS5_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP9777490.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(17209..17856)
                     /gene="gpmB"
                     /locus_tag="ACHOS5_002699"
     CDS             complement(17209..17856)
                     /gene="gpmB"
                     /locus_tag="ACHOS5_002699"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP9777491.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            17908..18435
                     /gene="yjjX"
                     /locus_tag="ACHOS5_002700"
     CDS             17908..18435
                     /gene="yjjX"
                     /locus_tag="ACHOS5_002700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP9777492.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(18508..18834)
                     /gene="trpR"
                     /locus_tag="ACHOS5_002701"
     CDS             complement(18508..18834)
                     /gene="trpR"
                     /locus_tag="ACHOS5_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP9777493.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(18924..20861)
                     /gene="sltY"
                     /locus_tag="ACHOS5_002702"
     CDS             complement(18924..20861)
                     /gene="sltY"
                     /locus_tag="ACHOS5_002702"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP9777494.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            21068..22735
                     /gene="ettA"
                     /locus_tag="ACHOS5_002703"
     CDS             21068..22735
                     /gene="ettA"
                     /locus_tag="ACHOS5_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP9777495.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(22791..23075)
                     /gene="nadS"
                     /locus_tag="ACHOS5_002704"
     CDS             complement(22791..23075)
                     /gene="nadS"
                     /locus_tag="ACHOS5_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9777496.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(23077..23409)
                     /locus_tag="ACHOS5_002705"
     CDS             complement(23077..23409)
                     /locus_tag="ACHOS5_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9777497.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(23501..24733)
                     /gene="nadR"
                     /locus_tag="ACHOS5_002706"
     CDS             complement(23501..24733)
                     /gene="nadR"
                     /locus_tag="ACHOS5_002706"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP9777498.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(24754..26136)
                     /gene="radA"
                     /locus_tag="ACHOS5_002707"
     CDS             complement(24754..26136)
                     /gene="radA"
                     /locus_tag="ACHOS5_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9777499.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(26185..27153)
                     /gene="serB"
                     /locus_tag="ACHOS5_002708"
     CDS             complement(26185..27153)
                     /gene="serB"
                     /locus_tag="ACHOS5_002708"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP9777500.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            27259..27903
                     /gene="ytjB"
                     /locus_tag="ACHOS5_002709"
     CDS             27259..27903
                     /gene="ytjB"
                     /locus_tag="ACHOS5_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP9777501.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            27931..28947
                     /gene="lplA"
                     /locus_tag="ACHOS5_002710"
     CDS             27931..28947
                     /gene="lplA"
                     /locus_tag="ACHOS5_002710"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP9777502.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISLDKTPDLPNFTETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            28979..29242
                     /locus_tag="ACHOS5_002711"
     CDS             28979..29242
                     /locus_tag="ACHOS5_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9777503.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
BASE COUNT         6968 a         7969 c         7233 g         7090 t
ORIGIN      
        1 tcgtgcaaat tcaatatatt gcaagaagat cgtaggcctg ataagcgtag cgcatcaggc
       61 agttttgcgt ttgtcatcag tctgatcccc ccgcctgggg ggattttttt attcgctaac
      121 ttcgcgtctt gcactcttcg gtctgaatgc tgcaaccact tccgctgccg tttcaacgta
      181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gcaccacaac
      241 gttaccctca gcgtccttca caccttccag cgtctcttta atagacttcg gttgaccggg
      301 taagttaagg atcagcgcct gtttgcgaat cacccccacc tgacgcgaaa ggatcgcagt
      361 tggtacaaaa tgcaggctga tctggcgcat ctgttcacca aagcctggca tctcgcggtc
      421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
      481 ggtgagcacc agatggcaac tcatttcatc caccagctca cacaacgttt gctcgatgat
      541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
      601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
      661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
      721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggtttcaga agaggtagcg
      781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
      841 tttttcctgg tcaacagcaa acttacggat accttccgcc agtttatcta ctgccattgg
      901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
      961 ttcaccagtg taagacagtt tacgttcgat agccccttcg ctctccgcca gctctttcag
     1021 cagtgccggt gcgatggtca gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     1081 acggaagctt gcgcccataa ccacggtttc ataaccatgc tctttgtagt actggtagat
     1141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcggtatt
     1201 cgctttgtac cagtcaagaa tacggccaac aaacggcgag atcaggaaca cgcccgcttc
     1261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     1321 tttttccagc tgttccgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     1381 acgatcgttg ctaatacctg catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     1441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     1501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     1561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     1621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     1681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     1741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     1801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     1861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     1921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     1981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     2041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     2101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     2161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     2221 catttcagtc attgtgtacc agtcttgcag cgcgcgtggg tagcggcaac ctggccggcg
     2281 ttgcgctggc aattaccgcc ggtggacctg gagccgtctt ctggatgtgg gttgccgcat
     2341 ttatcggcat ggcgacctcg tttgccgaat gttccctcgc acagctttat aaagaacgtg
     2401 atgccaatgg gcagtttcgt ggcggaccgg catggtatat ggcgcgcggg ctggggatgc
     2461 gctggatggg cgttctgttc gccgtctttt tgctcatcgc ctacgccata attttcagcg
     2521 gagttcaggc gaacgccgtt gcgcgagctc tgagtttttc ttttgatttt cccccgctgg
     2581 taacaggtat tattctcgct gtctttgctc tgctggcgat cactcgcggt cttcatggcg
     2641 tcgcccggct catgcagggg tttgtcccgt tgatggcgat aatctgggta ctgaccagcc
     2701 tggtgatttg cgtaatgaat atcgggcaac ttccccacgt catttggtct atttttgaga
     2761 gtgcttttgg ctggcaggaa gctgcaggcg gcgcggcggg atatacctta agccaggcga
     2821 ttactaacgg ttttcagcgc agtatgtttt ccaatgaggc gggaatgggg tcgacgccaa
     2881 acgcggcagc ggcagctgcg tcctggcctc cgcatccggc agcgcaagga attgtccaga
     2941 tgattggcat ttttatcgac accctggtca tctgcacggc aagcgccatg ctgatattac
     3001 tggcgggtaa cggcacaacc tacatgccgc tggaaggtat tcagcttatc cagaaggcga
     3061 tgcgggtgct aatgggttcc tggggtgcgg agtttgttac cctcgtggtt attctgtttg
     3121 ccttcagctc catcgttgcc aactacattt atgccgaaaa caatctcttc tttttacgcc
     3181 tgaacaaccc taaagcgatc tggtgtctgc ggatctgcac cttcgcaacg gtcatcggcg
     3241 gcaccttgct aagttttccg ctgatgtggc aactggcaga tatcattatg gcctgcatgg
     3301 ctattaccaa tttgaccgcc atattactgc tctcgcctgt ggttcatacc attgccagtg
     3361 attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg
     3421 agatcggtcg ccagctttct ccagacgcgt gggatgatgt ttcgcaggag taatcacaac
     3481 tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg caaggactgg
     3541 atatgctgat tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca
     3601 cgcgctatac gctgccggag ctgttagaca actcccagca gctgatccat gaagcgcgga
     3661 aactgacgcc cccgcagatt agctccctga tgcgcatcag cgacaaactg gcgggtatca
     3721 acgccgctcg ctttcatgac tggcagccag atttcacacc ggagaatgcc cgccaggcaa
     3781 ttctggcgtt taaaggtgat gtctacactg gcttgcaggc cgaaaccttc agcgaagacg
     3841 atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc gtactgcgcc
     3901 cgctcgattt aatgcagcct tatcgtctgg aaatggggat acgtcttgag aatgcccgag
     3961 ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac gaggcgctcg
     4021 cagcacaagg cgataatgta gtgataaatc tggcgtcaga tgagtatttt aaatcggtga
     4081 agccgaagaa attgaatgcc gagattatca agccagtgtt cctcgatgag aagaacggca
     4141 aatttaagat aatcagcttc tacgctaaga aagcacgcgg tctgatgagt cgtttcatta
     4201 ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa ggttacttct
     4261 ttgatgaagc ttcctccagc aatggcgaac tggtgtttaa acgctacgaa caacgttaag
     4321 tttgcagtaa atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcatgcagat
     4381 tcaatatatt gcagaattaa cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct
     4441 gaatgccgga ttgttacccg gcatttgtca tttagcggtg atgtttgcct ggaccatgac
     4501 cgccgtgatg atcatgagga gctttcttat ggtggcgcgg cgatggcggc ggtccatgtg
     4561 ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg tcgcgccagt
     4621 gaccgccatc ccagtaatag ccacgattat cacgatcgcc tatctgtaat tttactgacg
     4681 ggactagcgt aatttccgca gcctgcgctg ccatgggagc gaccagaacc agggaaagtg
     4741 cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc ccgttatggg
     4801 tcgatatccg gagattacgc gatgagaacg gcagttgtta ttctccgcaa tcctaatctc
     4861 taagttaccg catttattga gaatttctcc tttttccctg tcattttcct ccatattttc
     4921 ttcataaaaa agccgggctg cataaaagca aacccggcct gattgagata atgaatagat
     4981 gttactgatg attcatcatc aatttacgca acgcagcaaa atcggcgggc aggttatgcg
     5041 aaagcaaggg taaatcagca cgttctgcca gctcttttgg cagatccaac gtttcaccga
     5101 gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat
     5161 attcgcctgg attcaactgg tcacgcagcg cacgataagc tacggcagcg tgcggctccg
     5221 aggtatagcc cagttctttt aactcacgca ttgtctgttg cgtggtttca tcatccacgg
     5281 ctgcataccc cagctctttc agttgccaga ttttgcggcg gaacaactct tccacacgcg
     5341 gccagttgtt tggctggcta acatccatcg cattggataa cgtcgcctga gtcgctttgg
     5401 gtgaccactg accgtcgtgc aggaaacgtg gtacggtatc gttcacgttg gtcgcagcaa
     5461 taaaacgctt caccggcaga ccgagtgact tcgccagcag gcctgccgtt aaatcaccga
     5521 agtttccact cggtaccgag acaaccaact ggttacgtgc ttcttgcggc aactgtgcga
     5581 cagcctcaaa gtaataacaa atctgcgcca gcaatcgact gatgttgatg gagttagcag
     5641 aattcagccc cagcgccact ttcagttctt catcatcaaa cgcctgcttc accagcgcct
     5701 gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat attgccgccc aatgtacaga
     5761 acagtttttc ttgcagtgga ctgattttgc ctcgtggata gaggataacc actttcacat
     5821 tcggtaaacc gtagaaagca tgagccactg ccgctccagt atcaccggat gtcgcggtca
     5881 gaatggtcac tggcttatcg cccgcaatat gggtcagcat ttgtgccata aagcgaccgc
     5941 cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg acatcgcttt
     6001 caacattggc gaccggagcc gggaaggcaa acgccgcacg tacgcgctct tccaggattt
     6061 cctgcgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg gtgacaaaat
     6121 ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa
     6181 acagcccctg atttttgcct aacccctggg ttacggcttg cgcaaagctg acctgctcat
     6241 tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg cccgccgtat
     6301 ccagctggca aatatgaaca aaaccttcct gattttgcag gtagttctta cccaaccagt
     6361 cggcaacgcg ctgggcggta tccggcttgt cacacagagc gaacaaggtc gggccggagc
     6421 cggagatacc gctcgctacc gcgccgattt ccgcaaccgc ctgccgcgcc tgccggaagc
     6481 caggcaataa ccgttcacgg tagggttcag cgataacatc tttcatcagc ttcgcggcaa
     6541 gctcaggctg acgggaatag caggcgtgaa tgaagcctgc caaatgtcgc ccgtgcgcaa
     6601 tgcaatcctg gcggcgatac tgcgccggta aaatagccct ggcttctgcc gtcgagactt
     6661 taatccccgg atacgccagc acccacagcc actcatcaaa cccaggcact tgctggctga
     6721 tgatgtcgtt ttcttcgatc atcaactgca taccaccaag aaaacacggt gccacgttgt
     6781 cgtaatgaat gctgccggag atacgccctt ccagctcgcc catcaaagcc agcaaacgag
     6841 tgtcattaag cggcttgcca cagtgttcat tcatcgccat cagcgccgcg accaccgaac
     6901 aggcgctgga gcctaagccc gaaccgatcg gcatattctt ttccagagtc atcgccactg
     6961 gaatttgctt gccaagctcc tggcaaaaac gctcccagca ctgataaacg atattttccc
     7021 gtggctctga cggcagctta tcggcaaagc gtccgaggtt gttgagactg aatgtctctg
     7081 ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacaggtgtc accgccgccc
     7141 cgagcacatc aaacccgacg ctcatattgg cactggaagc cggggcataa actttaacca
     7201 tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac accggcagct
     7261 gtaacgtcat tgcccgcacc atatccgcgc agtaccaacg gcagcggctg ataatagtgg
     7321 ctatagaagg ccaaggcgtt ttcgccattt ttcactttga acagcggatc attaccatcc
     7381 acttcggcaa tcttcacgcg gcagacgcca tcttcatcaa tattgccaac atagcgcaaa
     7441 acttttcctt catcacgggc cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac
     7501 agattcgcca taaaagcggc aacatcaccc tcggcgttaa actctgcggg cagcacaggt
     7561 tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag aatcaatagt
     7621 ttacgcgcca catccatacc agaaagatca tctcgcgggt ccggttcggt ataacccatt
     7681 tcccgcgcca gcgtggtcgc ctcggagaaa ctcatgcctt cgtctaactt gccgaagata
     7741 taagaaagcg aaccagaaag aatgccggag aacttcatca attcatcacc tgcattgagc
     7801 agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc atagaggaat
     7861 ttacgccgcg atttttccgc cgcatgacgc aacagatggt agtaatccat cgacgaggtg
     7921 ttagcctttt tgttcggcgt aacaacgtgg aaaccttcgc gcaggaagtc ggcatattga
     7981 tccgccactg cctggctgga agtacagtca acaatgaccg ggttcagcag atgatattct
     8041 ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgcgc cagttcttcc
     8101 tgccagtttt ccagattaag gccatgtaca ttggtgagca gtgccttcga gttagcaaca
     8161 ccgcagacac gtaagtcgat atgtttattc ttcaaccagc tttgctgacg cttcagttgc
     8221 tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat aacctgatcg
     8281 gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc gttattgacc
     8341 acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt gatattggcg
     8401 cgggccagcg cggcaaagaa tttcgccgag atcccacgta aggtgcgcat accatcacct
     8461 accaccgaga taatggccag ccgttccgtc accgccaacg gctccagtaa gccttctttc
     8521 agttccaggt agaactcttc ctgcattgcc cgttcagctc gcacacagtc gctttgcgga
     8581 acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg
     8641 gcgcgtgaca tcgctgcaaa gacgcgcgcc gccatgccaa ccatcccttt catccccggg
     8701 ccggaaacgc tgaacattgc catgttattc agattggaaa tgcccttgac cggtaattcg
     8761 tcttcatcac ggctggcacc aatgagcgta cctggtgctt gaggatttcc ggtattttta
     8821 atcaggcaag ggatctggaa ttgggcgatg ggggtaatgg tgcgggggtg aagaacttta
     8881 gcgccgaagt aagaaagctc catcgcttcc tgataggaca tcgacttcaa caacctcgca
     8941 tcgggcacct gacgcggatc gcaggtataa acaccgtcaa catccgtcca gatctcgcaa
     9001 caatcggcgc gtaaacaggc cgccagcacc gcagcggagt agtcggaacc gttgcgtccc
     9061 agaaccacca gctcgccttt ttcattaccg gcagtgaaac cagccatcag caccatgtgg
     9121 tcagccggaa tgcggcttgc cgcaatacgg cgggtggatt cagcaatatc aacggtagat
     9181 tcgaggtaat gccccactgc aagcagtttt tcgaccggat cgataacggt aacgttgtga
     9241 ccacgcgctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc acggcaaatc
     9301 agcgcagcgt tgatgctatc cgggcactgc cccaacaaac tgatgccatg caggacatgt
     9361 tttatttggg caaattcctg gtcgacgaaa gttttcaatt gtgccagcgg aaatcccggc
     9421 tgggcggcgg cgagtcccgt cagaagttcg gcaaaaatac gttcggcatc gctgatattg
     9481 ggtaaagcat cctggccgct aatggttttt tcaatcatcg ctaccagatg gttggtgatt
     9541 ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct ttccagaata
     9601 tcggcaaccc gcagaaaacg ttctgcattt gccactgatg taccgccgaa cttcaacact
     9661 cgcatggttg ttacctcgtt acctttggtc gaaaaaaaag cccgcactgt caggtgcggg
     9721 cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg
     9781 tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct
     9841 gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta
     9901 atttactcac ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca
     9961 cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg
    10021 ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt
    10081 ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc gaccagtttt atgtcatctg
    10141 ccactgccag agtcgtcagc aatgtcatgg ctcgttcgcg taaagcttgc agttgatgtt
    10201 ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat
    10261 agaccatcac tgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag
    10321 taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca
    10381 ccagagcggc atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg
    10441 gcgtagcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcaaca gtgaaatcga
    10501 catcgtgtaa cgattcagct agtgtcttga aaactttaat attatcaata atatcaccag
    10561 atccatgtgc gacccaacgg gcggctggct ccaggtgtgc ctgactatca acaatccgca
    10621 gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg
    10681 cgggtgcaac cagaataatc gttatacgca tattgccact cttcttgacc aaataaccgc
    10741 gaacagggtg atcattgtca acttattacg cgttgcgaat ttacaaattc ttaacgtaag
    10801 tcgcaggaaa agccctttgc ttagcttaaa aaaggctaaa ctatttcctg actgtactaa
    10861 cgattgaatt gttaaaaaat gctacatatc cttctgttta cttaggataa ttttataaaa
    10921 aataaatctc gataattgga ttcaccaggt ttattagttg tatgatgcaa ctagttggat
    10981 tattaaaata atgtgacgaa agctagcatt tagatacgat catttcatca aactgttaac
    11041 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtcctgcccc
    11101 tcttagcctg ttatattgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
    11161 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
    11221 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
    11281 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
    11341 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
    11401 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
    11461 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
    11521 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
    11581 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
    11641 gactacatca ccaaaccgtt caacccgcgt gaactgacta ttcgtgcacg caacctgctg
    11701 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
    11761 ttcaatggtt gggaactgga catcaacagc cgttcgttga tcagccctga tggcgagcag
    11821 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
    11881 attcagtccc gtgctgaact gctgaagaaa atgactggcc gtgagctgaa accgcacgac
    11941 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
    12001 ccggaaatca tcgccaccat tcacggtgaa ggttatcgct tctgcggtga tctggaagat
    12061 taatcggctt taccaccgtc aataaaaacg gcgcttttta gcgccgtttt tatttttcta
    12121 tcttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    12181 ggcagtgaaa acgcatacca gtcgatatta cgggtcacaa acatcatgcc ggccagcgcc
    12241 accaccagta cactggttcc caacaacagc gcgctatcgg cagagttgag cagtccccac
    12301 atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattacg ccagcctttc
    12361 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    12421 gccacagtaa aaccggtatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    12481 gacagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    12541 acaaagaatg ccataaaagt cagcgcaatc agcagaatgg cgtacttagt cgcccggtcc
    12601 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg gaagttttcc
    12661 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctactt
    12721 tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggtag aaaatcacct
    12781 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctataacg cccgccaggc
    12841 accacagaaa gatcgccggt accgcttaaa ttcagcgcca cattcagctt caggttctgc
    12901 ttgcgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    12961 gtgccgggtt caatggtcag cgccgtaccg ttaacttcag gcgctttcac cacaccaata
    13021 ccacgcgcat ccccgacgct aatcacaata aatggcttgc ctaaggtgat atttggcgcg
    13081 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    13141 acctgaccgg tatagatgcc tatcttgcgt tcttccacat tctgattgcc atcaaccatc
    13201 aatgactcag gtaaccaaaa atgaataaaa cttcgtttcc gctccacggt tttatcgtct
    13261 tcctgcaccg tataaagctc ggtcacagga atagcgatga gcggcccaac gagtttttgc
    13321 ggcccgctgg tactttggcg aatcacatct tccacatcgc tacggtaatc ggcacgttcg
    13381 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacaag
    13441 gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc aatgacagca
    13501 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
    13561 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    13621 gcgacctcac tgacaaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    13681 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatccc actaccggta
    13741 tccagcacct taagggtgac gtattcctga tccacttcgg cgcttagcgt tatgcaaccg
    13801 ctctcgggag taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg ctccagtaac
    13861 gccggttcag aagcaacgtt aacctcagta ggcataacat gcaaagtgat gtttttttct
    13921 gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac atcaacagca
    13981 gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagt
    14041 gcctgcattc gcgcattttg cgtcagaatg ttgtcggtaa aacgagccac cacttccggc
    14101 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    14161 tcatgagtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    14221 atactttcca gcgcctgcgc gagtttacgc aactcgctac taccgagatc ggggagagga
    14281 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gcctggcaat agagcggttg
    14341 atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccaacaaaat ggcgctggcc
    14401 cataaaattc gccgctcgct acgcttaatg accggagcca tcgccgcgtt cggtttgcct
    14461 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcaca
    14521 gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg accacgcaac
    14581 gttagccaga cgtcattcca gcgcgaataa tcctgtccaa cggctttatt tgccgaatcg
    14641 aacaatactt tgccctgcgc atcggtcata tagacatgat attcgttgcg cactttgtta
    14701 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
    14761 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
    14821 gcggtgtcga ttaacgtccc ctcggttgct cttcgcacgc ccggtttaac ttctttgaca
    14881 aaaatagcca gaacgaacca ggccgccacc gccaccagta aaaaatagcc cagcaacaac
    14941 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
    15001 tcggtgaaag atcggggttg atggcgcgca gcttggcacg cagcgtttta atgtgggtat
    15061 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatc agttgctggc
    15121 gggaccagac gcggcccggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    15181 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc aggttcattc aattcaaaat
    15241 gaccaatacg gataacggga gacggcgtcg agaacttctt cacccgacgc agtaaggtgc
    15301 gcactctggc gcacacttcg cgaggtgaaa acggtttagc cacgtagtcg tcagcgccaa
    15361 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    15421 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
    15481 caacatcgag aatcatgaca tcgggaacct gctggcgagc tttatccagc actggcaagc
    15541 ctcgctcaaa gacctcgacg tcaaaacctt cctgttgcaa catgtagacc agcgtatcgg
    15601 ctatcccttg ctcgtcttcg actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    15661 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    15721 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt agcgtcataa aagcgcacga
    15781 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    15841 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgttgacaa gaaatggccg
    15901 catcggaggt atcttctgcc aggcccaatc cccctttaat accaccggtt ttcgcccggc
    15961 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttcaaca acaattttgt
    16021 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    16081 atggccccag cattatcagg cttaaagaca ggattaaatg cttgtatttc atatcgttac
    16141 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    16201 caaaatctca atacttttat ttccgtcaag acctaaaaca tactcgacta agaaaaaaac
    16261 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    16321 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    16381 gctggaaggt catctggatc atcccctgtc gctcgacaat gtagcggcga aagcaggtta
    16441 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    16501 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    16561 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgctttcaa
    16621 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    16681 tattcgcccg ccgctacgtc tgggtgaatt cactatgcca gagcacaaat ttgtcactct
    16741 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    16801 tgatttccgc catgaaatgc gctaccagtt ctggcacgat tttctcggca actcgccaac
    16861 cattccacca gtgctttacg gtttgaacga aacacgtccg agccaggata aagacgacga
    16921 acaagaggtg ttctacacca ccgcgttagc tcaggatcag gcagatggct atgtactgac
    16981 tgggcatccg gtgatgttgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    17041 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgcttaacct
    17101 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagatg ccaaagcggg
    17161 agatcgccca attaatctgc gctgtgaact gctgattccg atccgtcgtt aacgctgcag
    17221 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa ccacccagcc
    17281 agacgccagc cacaagcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    17341 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccaggc atcccagtgc
    17401 aataccatgg ctcaccagta gcggtcggct tccctgcggt aagtcccggc aagattccag
    17461 tgccgcatta acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    17521 aacggtgcca ttgaccagtt gccgacgcca gttctcttct tcttcggtga gtgaatcgat
    17581 atttcttgtt tccagtacac ccatatttaa ttcacgcagg cgagaatcaa agatgatgtc
    17641 acagccgcag gcctgggcga tgatttccgc cgtgcgccgg gtccgtccta aatcgctact
    17701 gatgatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    17761 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    17821 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    17881 acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac caccaatccc
    17941 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    18001 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
    18061 cgcgctggcg cacgaaatag ggtagccaat gcccgccgtt tacttcctga ggctgatttt
    18121 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgatagaa
    18181 aacgccagcc agcgcggcga ggcgcgttcg gctactttgc cgttgcctgc ggtgattctg
    18241 gaaaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    18301 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcacc
    18361 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcataatgc cgtgtatcag
    18421 ccattgcagg cctgattagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
    18481 gcgccatgtc ttatcaggcc tacaaaatca atcgcttttc agcaacacgt cttccagcca
    18541 ctggcgcagc tcgacgggcg cggctttcag actattagat ccacgcgtaa tcgtcgcgat
    18601 gcccgcgccg agttcatttt ttaactcacg ctggctcatt tcgccgcgca acagctcttc
    18661 gacaatacgc acgcgagtcc ccaacgcttc gcgctcatct ggcgtcagca tcaggtttaa
    18721 caacggtaaa tggagatcgt tttggtaggc attcttaagc aggtcgacaa aacgtaacca
    18781 ctcctggtga cgctgttctg ccatcgctgc tgaatagggt gattgttggg ccatagtatg
    18841 tcgccattgt tagcggggga agcaaaatgc ctcccccggt tgtactcgct aaggagtacg
    18901 atagcatatc ataaacgtgt ggatcagtaa cgacgtcccc attccgtggc gctcatcaac
    18961 gtcggtttat cccccatgaa atagcggtag taagcgtcat aagccagcac gtttttcaca
    19021 taaccgcgcg tctcggagaa tggaatactc tcgacaaatg ccactgcgtc gatacgccct
    19081 gcgctgttgc caagccaggt ccgcacccgc cctggtccgg cgttataagc tgctgaggag
    19141 aaaatacggt tattgccaaa ctgctgataa acatattgca ggtaactggt gccaatgttg
    19201 atgttcgttt ccggatccag caattgccca gggctgctat aaccgggaat agagaacatc
    19261 ttcaccgtat gggtcgcggt accaggcata atctgcatca ggccgctggc ccctaccggt
    19321 gatttcactt ttggattcca ggcactctcc tgacgggcaa tcgccatcgc atagctttgc
    19381 gggatctcct taccactggt gtagcgtttg aaaagatcgt tgtaagccag cgggaatcgc
    19441 tcttccagat gatcccacag cttcccggcg atcgttgcct gaacgctaag atcccaccat
    19501 tggttattga aagcataccg agccagttga gcctgctctg tttttgactt gctcttcacc
    19561 agattggccc actcgctacg cgcggtgtta tcgagattcc agtacatcaa ctcgcgcacg
    19621 cgcgccatct ccggcccctg agtcagggcg ctgtcaacat tctgcggcgc tttatcaatc
    19681 ttcagctcat actcttcgcc gatgcgttgt gcagcaacca tcgggtagaa accacgctgt
    19741 tgcatgagtt gatgcaaaat ctcttttgct tcagcttcac gtccgcgttc cagcaataaa
    19801 tccgcctgcc agtaacgcca ttcatctttc tctttcgctt ccataggcag acgcgccagc
    19861 caggtattca ggccgcgacg atcgccggtg ccaagcgcca ttcgcacacg gcgttcaata
    19921 agcgaagtag attgcgagcg cataatcgcg tcatcgcgcc atttcgcctg ctcgtcggtg
    19981 acatcgttgc ccatcaaacg ccaggcgaca atatcgcgca gctcctgaat ctgatcttca
    20041 ttaagctgct gtgcctgagc aagcgacggg atcatcagcc gtgcattttc agcatcctgc
    20101 cgcgccacac tggcaaacgc caccgccgcc atttgacggg taaaatcggt cgcgccggtt
    20161 gtacgcgcga aggtcagtac cgtattaggg ttgttcgcca gtgaaatgat tgccgaagcg
    20221 atagtctggt aatcggcagg catctgccct gccagcactg ttaccaggcc ggtgttaccc
    20281 gctttcatcg ccagacggat acgctctaaa tacgccagcg gatcttgttt acctgacgca
    20341 cgccagacgc taaataactt gtcacaggcg ttaggctggc tcttgccggt tagccacagc
    20401 tctttcgccc cttgccaggc ttcttcactc tgcccggtgt tccatttcgc atagtagtaa
    20461 ttacattgcg cttcggtagt tccgggcttt tccgggctaa aggctaacaa gccacgccag
    20521 tcttcacgcc gcgccagttc attgacgaaa cgagattgca gcgtgcgagc gggaggaagc
    20581 gtggggttag cgcgaacaaa gttagtgacc gtcaccgccg gttgattcat caggtcatca
    20641 gtgatctggc ggtattccag gtagggataa agtgggtaat ctttcagtcc aggcatcatt
    20701 tgttcgacca catccatttg tcgattatcc caggcctgct taatttgcgc gtaacggcta
    20761 cgctgctcat ccagtgagtc ggctcgcgcc acgctactga ccgtcagcag acagacaccg
    20821 acagccaaca gccgccaggt aacgtgtttg gctttttcca caagcgcatc ctctaagtgt
    20881 aaatacatca atgcagcgtc atgccgcgta atagatttat gctaaccagt cattgccgtt
    20941 tacgccacgt tacggacact tttttacttt tacggggtga tcgactggct gggattagac
    21001 agggaaacag gctacacttc gcctttgaaa actcatcatc acgataaaca tagaggcgaa
    21061 gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa
    21121 acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct
    21181 gggtctgaac ggcgcgggta agtccaccct gctgcgcatt atggcgggca ttgataaaga
    21241 catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttatctgc cgcaggaacc
    21301 gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt ctgaagtggt
    21361 taacgccctg aaacgcctgg atgaagtgta tgcgctgtat gccgatccgg atgccgattt
    21421 tgacaagctg gccgctgagc aaggccgtct ggaagagatc attcaggctc acgacggtca
    21481 caacctgaac gttcagctgg agcgtgcggc ggatgcgctg cgtctgccgg actgggacgc
    21541 gaaaatcgct aacctttccg gtggtgagcg tcgtcgcgtg gcgttgtgcc gcctgctgct
    21601 ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg cggaatccgt
    21661 agcgtggctg gaacgcttcc tgcacgactt cgaaggcacc gtggtggcga ttacccacga
    21721 ccgttacttc ctcgataacg ttgcgggctg gatcctcgaa cttgaccgcg gtgaaggtat
    21781 tccgtgggaa ggtaactact cctcctggct ggagcagaaa gatcagcgtc tggcgcagga
    21841 agcttcacaa gaagcagcgc gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca
    21901 gggaactaaa ggccgtcagt caaaaggtaa agcacgtctg gcgcgctttg aagaactcaa
    21961 cagcaccgaa tatcagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg
    22021 tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctatggtg atcgtctgct
    22081 gattgactcc ctgagcttct cgatcccgaa aggagcgatc gtcgggatca tcggtccgaa
    22141 cggtgcgggt aaatcgacgc tgttccgtat gatctctggt caggaacagc cggacagcgg
    22201 caccatcact ttgggtgaaa cggtgaaact ggcgtcggtt gatcagttcc gtgactcaat
    22261 ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatca tgaagatcgg
    22321 caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag gggttgatca
    22381 gggtaaacgc gttggcgaac tctccggcgg tgagcgcggt cgtctgcatc tggcgaagct
    22441 gctgcaggtt ggcggcaaca tgctgctgct cgacgaaccg accaacgacc tggatatcga
    22501 aaccctgcgc gcactggaaa acgccctgct ggagttcccg ggctgcgcga tggttatctc
    22561 gcacgaccgt tggttcctcg accgtatcgc cacccacatt ctggattacc aggatgaagg
    22621 taaagttgag ttcttcgaag gtaactttac cgagtacgaa gagtacaaga aacgcacgct
    22681 gggcgcagac gcgctggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt
    22741 aaaatgccgg atgcggcgca ccagcatcgc atccggtatt agttaaatga ttaatgggaa
    22801 gcaagttccc ggaaaaaggc aggattttct ttaattgtgg caagtacttt tgcagccaat
    22861 cccgtaggat ttcttcgctt cgattcccag cttttaatgg tatccacact ggttcccaga
    22921 gcttttgcca tctcactttg agaaacgttg agttgctcac gaatagcttt aacatccgca
    22981 atttcgtagc gggtcacccg tgcaggttct tttaaacctt gtttaatctc gacagcctct
    23041 tccagagagg ttttcaactc atcaaaaaaa ctcattttat atctcgcctt tgagcagttt
    23101 cgtcaacttc tttagttgag ctttttccgt atctgtcaga ctatctttgg catttttcgg
    23161 ataggccata atcaaataaa taatctcttc cgtcgccaga aagtaaatta ttctgacact
    23221 gccacttttt ccctgtgaac ccgcagccat tcggacttta cgcaatccac cagtctgctg
    23281 aatgagatca cctttatcgg gagttccgat gagttctttt tgtaattcct ttaattcatc
    23341 atcggttgca atctgcttga tctgacgcgt aaacattggc gtctctataa atattatcgc
    23401 cttacccatt ccccctccct ggcaggtaca ttgtacacct aaatggagta ctaagtacac
    23461 ttttaaatca gtaatcccac tcaacatctt ataaagatga ttatctttgc tcccccatca
    23521 tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc tcttcaaccc
    23581 gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg aactctttgc
    23641 gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg ttgttctcca
    23701 gcaggatcac cagatcgaaa cggtattcat caatcaacgc ctgcacgaac ggatgctcac
    23761 gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtcg ataaacgcca
    23821 ctttattggc atatttcact gcaaaatcaa tatattgcgc gtgtcccagc gcgattttat
    23881 cgtagtcgga atactgcaac gcaatctcat caccgccaag gtgtgaaaag acataatcgc
    23941 gaccatattc ccatgcactg gtagtgttga agatattggc aagtttgttt actagggtgg
    24001 atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaagaac ggcttaactt
    24061 ccgtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca ccgctgatac
    24121 tcataaaggt acgcttcgga tcgaccagca ccgtctcgat ccccagatgt tccatatact
    24181 gcggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctgaatgcct ttttcggcca
    24241 taaacttttt gatgccgttg ctccacacat cccagccatg tggatacggt tccatgccct
    24301 cttcgttaaa agcatgaatg cggatatttt tttgatactt aaaggtctgc aataaccagc
    24361 gcagacgatc cggcacggtt ggctgctgcg acatggcgct gtcttcgaac aatgcgcggt
    24421 cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta caggcgcgct
    24481 ggataaggta gatatgtcca gtatgcagcg ggtaaaactt accgaatacg acaccgatcg
    24541 ttttcttctg ccggggaaat tcaagtccca aataacggtg caatgcctcc agcttttgtg
    24601 cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg gtcataccgc
    24661 tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat
    24721 caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca cgctaagcgc
    24781 gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt tcggcacgtt
    24841 agctgccgga acaattgccc ggcgaaaacc gtgtttcgcc gcttctgaga ttcgttcctg
    24901 gccgctgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca ccaccagatc
    24961 ctgcggtagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg ctaaatcggc
    25021 actggtttcc gtcaccttca cgccaccgac cacgttaaca aacacatcct gatcggccat
    25081 ttgtagacca ccgtgacggt gaagcaccgc cagcaggatt gccagacggt tttgctccag
    25141 cccgactgcc acgcggcgtg gattcgccat catcgagtga tcgacgagcg cctgaatctc
    25201 caccagcagc ggacgcgttc cttcccacac taccatcact gagctaccgg aggtcacttc
    25261 atcgccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc cctgctcggt
    25321 cattgcgaag acacccagct cattcaccgc gccgaagcgg tttttatggc tgcgtaaggt
    25381 gcgaaaacgt gagtcggcat caccatccaa aagcaccgaa cagtcgatac agtgttccag
    25441 cactttaggg ccagccagcg agccatcttt ggttacgtgc ccgaccatga caatcgccac
    25501 accgcgcgtt ttggcgaagc gcgtcagata agccgccgtt tcacgcacct gtgccacgct
    25561 gccaggcgat gattgtacat ccgccatatg catcacctgg atggagtcaa ttaccatcag
    25621 cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt ccgacagcat
    25681 attaagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct gttgcagcga
    25741 ctcttcgccg gtgacataca gcgttttcat ctgctgcgcc agtttgcaca gcgtttgcaa
    25801 cagcagcgtg gatttcccgg cacctgggtt accgccaatc agaatggcac ttcccggcac
    25861 cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc
    25921 ttcaaggctg atgtcggaaa gtttctggac ttttgccact ccggcgctac cggcatagcc
    25981 actgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct cggtgatggt
    26041 gttccaggca tgacaggcgc tgcactgccc ctgccagcgc ggataatcgg ccccgcattc
    26101 attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta atgctgtgcc
    26161 gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga ggatgcagaa
    26221 tacacccatc aggtcagcgt gacggatggt gacttccgtt ttttcattca cttttggctt
    26281 ggcatggtag gcaatcccca gccctgccgc tttgatcatc ggcaggtcat tggctccatc
    26341 gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc gagtcagagt
    26401 tttcgctttg tactgcgcgt caacgatgtc gccgatcaca ttgccggtaa atttaccgtc
    26461 catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc gcaggtattc
    26521 agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg tttccagctt
    26581 gagtaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct gttgcagaat
    26641 gttcgcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg taaaatcgag
    26701 ttcgccgcgc atcgcccgtt cggttacctc cgccaccctc tcgcccgttc cggccagttt
    26761 ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca tcaccagcaa
    26821 acccggcgtg cgcaggtgcg ggattttccc cagcggcgcg acatccagct gcgcttcgtg
    26881 ggccaggcgc gtagcccgcg cggtgagtga acctgccaga cgaatcacct gataatcttc
    26941 cacgcaccag gcggcaacaa tcaccatcgc cgcgcccagt ttgctctggt attgggtcag
    27001 acgttgttta tccagtccac gaccatacag cagccagccg ctacgacctg cgtggtaatc
    27061 cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata aagagacatc
    27121 ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat cgttcgaagc
    27181 agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat taagtctcaa
    27241 attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctgca tcgggcagtg
    27301 attgtcctgt tctgtcttgc cttgttagtg gcgctgatgc agggagcgtc atggtttagt
    27361 caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac cctggcgcgg
    27421 caggtgacgc taaacgttgc accgctgatg cgtaccgact caccggatga aaaacgcatt
    27481 caggcgatcc tcgatcagtt aacggatgaa agccgcattc tcgacgcagg cgtatatgat
    27541 gagcaaggcg atcttatcgc ccgttctggc gaaagcgtcg acgtgcgcga ccgactggcg
    27601 ctcgacggca aaaaagcggg cggctatttt aaccagcaga ttgtcgaacc cattgcgggt
    27661 aaaaacggac cgctcggcta tctgcgcctg acgctcgaca cccatacgct cgccaccgaa
    27721 gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct ctcgctggca
    27781 atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg gcagcaatcg
    27841 cccttcctgt taaccgccag taaaccggtg ccggaagagg aagaaagcga gaaaaaagag
    27901 tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct catctctgac
    27961 tcttacgacc cgtggtttaa cctggcggta gaggagtgta tttttcgcca gatgccggct
    28021 acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtgattgg tcgcgcgcag
    28081 aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg cctggcgcgg
    28141 cgcagtagcg gtggcggcgc agtgttccac gatctcggca atacctgttt tacctttatg
    28201 gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa tgcgctgaac
    28261 gcgctcggcg tcagcgccga agcttccggg cgtaacgatc tggtggtgaa aaccgccgaa
    28321 ggcgatcgca aagtctcagg ctcggcctat cgcgaaacca aagatcgtgg cttccaccac
    28381 ggcactttgc tgctcaattc cgaccttagc cgcctggcaa actatctcaa tccggataaa
    28441 aagaaactgg cggcgaaagg cattacctca gtgcgttccc gcgtgaccaa tctgaccgaa
    28501 ttgctaccgg ggatcaccca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc
    28561 cattatggcg agcgcgtgga agcggaaatc atctccctgg acaaaacgcc agacttgcca
    28621 aacttcaccg aaacctttgc ccgccagagt agctgggaat ggaacttcgg tcaggctccg
    28681 gcattctcgc atctgctgga tgaacgcttt acctggggcg gcgtggaact gcatttcgac
    28741 gttgaaaaag gccatatcac ccgcgcccag gtgtttaccg acagcctcaa ccccgcgccg
    28801 ctggaagccc tcgccgggcg actgcaaggc tgtctgtacc gcgcggatat gctgcaacag
    28861 gagtgcgaag cgctgctggt tgacttcccg gaacaggaaa aagagctacg ggagttgtca
    28921 gcgtggatgg caggggcggt aaggtaactc gctgattaaa atatccaggg aaggaaatat
    28981 gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc agggtatatc
    29041 ctggctaacc gccgtgcgta tcaggcataa actatcactt gctgaagtaa gtaaaatgtt
    29101 aggtatctcc attaattctc taaaacagat cgaaaagacg gaacggctta gttcaaacat
    29161 caaaagcaaa atggcaggaa tttatggttg tccaccagag ttgctgatat gcccttcctg
    29221 gatgactgct gaacataaat gaatagattc gtgaatagat
//
LOCUS       DAZKHK010000062        28752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKHK010000062 DAZKHK010000000
VERSION     DAZKHK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.062"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(21..1145)
                     /locus_tag="ACHOS5_002712"
     CDS             complement(21..1145)
                     /locus_tag="ACHOS5_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFP9777504.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTYAIEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKKYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHHDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1278..2831
                     /locus_tag="ACHOS5_002713"
     CDS             1278..2831
                     /locus_tag="ACHOS5_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9777505.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3213..4103
                     /gene="metF"
                     /locus_tag="ACHOS5_002714"
     CDS             3213..4103
                     /gene="metF"
                     /locus_tag="ACHOS5_002714"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP9777506.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRII
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4432..6612
                     /gene="katG"
                     /locus_tag="ACHOS5_002715"
     CDS             4432..6612
                     /gene="katG"
                     /locus_tag="ACHOS5_002715"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP9777507.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6706..7611
                     /gene="yijE"
                     /locus_tag="ACHOS5_002716"
     CDS             6706..7611
                     /gene="yijE"
                     /locus_tag="ACHOS5_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP9777508.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7638..8255)
                     /locus_tag="ACHOS5_002717"
     CDS             complement(7638..8255)
                     /locus_tag="ACHOS5_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP9777509.1"
                     /translation="MKASLVLFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8531..9634)
                     /gene="gldA"
                     /locus_tag="ACHOS5_002718"
     CDS             complement(8531..9634)
                     /gene="gldA"
                     /locus_tag="ACHOS5_002718"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP9777510.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9645..10307)
                     /gene="fsa"
                     /locus_tag="ACHOS5_002719"
     CDS             complement(9645..10307)
                     /gene="fsa"
                     /locus_tag="ACHOS5_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9777511.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10319..12820)
                     /gene="ptsP"
                     /locus_tag="ACHOS5_002720"
     CDS             complement(10319..12820)
                     /gene="ptsP"
                     /locus_tag="ACHOS5_002720"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9777512.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAYQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIAALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13129..14208
                     /locus_tag="ACHOS5_002721"
     CDS             13129..14208
                     /locus_tag="ACHOS5_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP9777513.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            14223..14543
                     /locus_tag="ACHOS5_002722"
     CDS             14223..14543
                     /locus_tag="ACHOS5_002722"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9777514.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14594..16891
                     /locus_tag="ACHOS5_002723"
     CDS             14594..16891
                     /locus_tag="ACHOS5_002723"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9777515.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQATLLLLEASQKHILRYAELAETMAASCT
                     DGPRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16857..17735
                     /locus_tag="ACHOS5_002724"
     CDS             16857..17735
                     /locus_tag="ACHOS5_002724"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP9777516.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17737..18078
                     /locus_tag="ACHOS5_002725"
     CDS             17737..18078
                     /locus_tag="ACHOS5_002725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9777517.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18065..18916)
                     /locus_tag="ACHOS5_002726"
     CDS             complement(18065..18916)
                     /locus_tag="ACHOS5_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9777518.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19142..20875)
                     /locus_tag="ACHOS5_002727"
     CDS             complement(19142..20875)
                     /locus_tag="ACHOS5_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9777519.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21058..23709)
                     /gene="ppc"
                     /locus_tag="ACHOS5_002728"
     CDS             complement(21058..23709)
                     /gene="ppc"
                     /locus_tag="ACHOS5_002728"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP9777520.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24011..25162)
                     /gene="argE"
                     /locus_tag="ACHOS5_002729"
     CDS             complement(24011..25162)
                     /gene="argE"
                     /locus_tag="ACHOS5_002729"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP9777521.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25316..26320
                     /gene="argC"
                     /locus_tag="ACHOS5_002730"
     CDS             25316..26320
                     /gene="argC"
                     /locus_tag="ACHOS5_002730"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP9777522.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26328..27104
                     /gene="argB"
                     /locus_tag="ACHOS5_002731"
     CDS             26328..27104
                     /gene="argB"
                     /locus_tag="ACHOS5_002731"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP9777523.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTNEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27165..28538
                     /gene="argH"
                     /locus_tag="ACHOS5_002732"
     CDS             27165..28538
                     /gene="argH"
                     /locus_tag="ACHOS5_002732"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP9777524.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         6684 a         7235 c         7863 g         6970 t
ORIGIN      
        1 tcgtttgtcg agtgtattga ctaacaagat ggagtgagcg attcattgta ttttaacgcc
       61 agtgaggtca gcatttgcgt tacgcgacct gcggataata acgcgctttg ggtaaagtcg
      121 tgttggtctg tcagccatgt gataaaacaa cgactggttg ccagagaaac atttgcgccc
      181 ctttctaagc aggctcccgt gagtaggcag accatcttgc agttattccc ctgctcccag
      241 cgctgccact gattttgcca ccaaccatgg tatgtcgttc ccttaaaatg ccagggcgta
      301 tcagcgttac tgtcactgat ttcttcttct acgaaccaaa gttcaccttg ttgttggcgg
      361 aactgatctg cccaggctgt aataattgcg cctcttagaa aacattcgtg tgaaataata
      421 tgcgttttga catctggata gcggattttc agtattctga aatgcccgtg tgccgtgata
      481 accgggcgta gtatggctcc cttaccgttg cgcttgagta cagcactgcc atactgaatt
      541 tcatcgaact ggctccggcg caagaattgc gcttcacgca tatctacctt ctgcacgata
      601 tcgtgatggt gcatagttgg ttctgtgttg cccttgtaac tgtaccgcag actgtctcct
      661 gtctgatgtt gcgtataatt cgtggagaga tgaagaatgt tgccacgagt ggcgtcaaga
      721 ctgactaaaa catacagttt ttgtactgcg ctggcccctt gaaagggtat gaacagttga
      781 acggtttcaa tggcctgggg atgcgtaatt tctttctgat attttttagg cgtccagact
      841 tttttgcagt tacggcattg aatgcgttgc gaaccctgtg gattgtgacc ataacaaatc
      901 atgtctgtgc cataacatac tgggcagaaa tggcctttct caattgcata agtagacaag
      961 tgaactgaca accagtcacg aaattgttgc tcgtcaaaca acggcgggta actgccacat
     1021 gcccgacagt gcagtgccgg ataacccagt ttatagtctg gccaactgta atcttccgat
     1081 gaggccaatc ccaaatttcg gcaaccgaac gttttacagg cattaatcgt aaacagagtc
     1141 gacatatatc accaacgatt tcacaaggtc gttgattttg tccggcaact atcccctgcg
     1201 gatgtgatta ctctcacata aataagtctt tcagaacgta atgtagcaat cctttatctc
     1261 actgaaaggg attgtttatg aaaattaaaa tacttgctgc gggtatcgca ttaacactgc
     1321 cgttctgggc ttgcgctaaa gatgtcacta ttatttatac caacgacctc catgcgcatg
     1381 tagaacctta taaagtgccg tggatcgctg acggtaaacg ggatattggc ggctgggcaa
     1441 atatcactac gctggttaag caagaaaaag caaaaaataa agcgacatgg ttttttgatg
     1501 cgggcgacta ttttaccggg ccatatatca gcagcctgac caaaggtaag gctattatcg
     1561 atattatgaa tactatgccg tttgatgccg tcacaatagg caaccatgaa tttgaccacg
     1621 gctgggacaa cacattatta cagttgagcc aggcaaaatt ccctattgta cagggcaata
     1681 ttttttatca gaacagcagt aaatcattct gggataagcc ctataccatc atcgaaaaag
     1741 acggtgtgaa aattggcgtt ataggtttgc acggagtatt tgccttcaat gatacggtat
     1801 ctgctgccac gcgcgtgggt attgaagcgc gcgatgaaat taaatggcta caacgttata
     1861 tcgatgaact caaaggcaag gttgatttga ccgtcgccct gatccacgaa ggtgttccgg
     1921 cccgccagtc cagtatggga ggcacagatg tgcgtcgcgc gttggataaa gatattcaga
     1981 cggcaagtca ggtgaaaggg ctggatattt tgatcaccgg acatgcacat gtggggacgc
     2041 cggaaccgat taaagtcggc aatacgctca tcctctccac tgacagcggc gggattgatg
     2101 tgggcaaact ggttctcgac tacaaagaga agccgcataa ttttacggtg aaaaacttcg
     2161 agcttaaaac catttacgct gacgagtgga agcccgatcc gcaaacgaaa caggtgatcg
     2221 acggctggaa taaaaagctt gatgaagtgg tgcaacaaac ggtggcgcaa tcgccggttg
     2281 aattaaaacg tgcctatggt gaatcggctt ccctcgggaa cctggcggct gacgccttac
     2341 tggcagcagc gggtaaaaat acccaactgg ctttaacaaa ctctggtggt atccgcaatg
     2401 agatccccgc aggggcgatc acgatgggcg gcgtcatcag taccttcccg ttccccaacg
     2461 aactggtgac gatggaactc acgggtaaac aattacgcag tctgatggaa cacggcgcaa
     2521 gtttaagcaa tggcgtttta caggtatcga aaggcctgga aatgaagtac gacagcagca
     2581 agccggttgg tcagcgggta atcacactga ctctgaatgg caaacccatt gaagatgcga
     2641 cggtttatca cattgccact cagagtttcc ttgctgatgg tggagatggt tttaccgcct
     2701 ttaccgaagg gaaagcgcgt aacacaacgg gcggttatta cgtttatcac gcggtggttg
     2761 attacttcaa agcgggcaac accattacgg atgaacagct caacggtatg cgcgtgaagg
     2821 atatcaaata aaagtgcgca ctgaattgac tcaactgttt gttacccgtt gattgcgcag
     2881 atcctgtatt cggcctacgc gagggcgcag gccgaatcag atgccttaac atcaccatcc
     2941 ggtggcagga cgttgttttt cgaattagcc caggtgtcgc tgtgttgtca gaatatgagt
     3001 caaaacgtgc gaggtggatt gcgctataga gaaatctttt atcgcctcta attaagatga
     3061 gtttttttca tcttccctga tttttcctca ccatcattgg ttatttttcg gttgacgccc
     3121 ttcggctttt ccttcatctt tacatctgga cgtctaaacg gatagatgtg cacaacacaa
     3181 catataacga taagcgattg atgaggtaag gtatgagctt ttttcacgcc agccagcggg
     3241 atgccctgaa tcagagtctg gcagaagtcc aggggcagat taacgtttcg ttcgaatttt
     3301 tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat cgccttagca
     3361 gcctgaaacc gaagtttgta tcggtgacct atggcgcaaa ctccggcgag cgcgaccgta
     3421 cgcacagcat tattaaaggc attaaagatc gcaccggcct ggaagcggca ccgcatctta
     3481 cctgcattga tgcgacgcct gccgagctgc ggatcattgc acgcgactac tggaataacg
     3541 gtattcgcca tatcgtggcg ctgcgtggcg acctgccgcc gggaagtggt aagccagaaa
     3601 tgtatgcttc tgacctggtg acgctgttaa aagaagtggc agatttcgat atctccgtgg
     3661 cggcgtatcc ggaagtacat ccagaagcga aaagtgccca ggccgattta ctcaacctga
     3721 gacgaaaagt ggatgccgga gccaaccgcg ccattactca gttcttcttc gatgtcgaaa
     3781 gctacctgcg ttttcgcgat cgctgcgtct cggctggcat tgatgtggaa atcattccgg
     3841 gcattttgcc ggtatctaac tttaaacagg cgaagaaatt tgccgacatg accaacgtgc
     3901 gtattccggc gtggatggca caaatgttcg acggcctgga tgacgatgct gaaacccgca
     3961 agctggtggg cgcgaatatt gccatggata tggtgaagat tttaagccgt gaaggagtga
     4021 aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt tgccatacac
     4081 tgggtgttcg acctggttta taaatagtgt gccttttgtg aaaatcacac agtgatcaca
     4141 aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata aggttatcag
     4201 ccttgttttc tccctcatta cttgaaggat acaaatctaa aacccttttt tataaagcat
     4261 ttatcctaaa tcggacataa tcaaaaagtc ttaattacga tcaatttgat ctacatctct
     4321 ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt
     4381 ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg
     4441 tcagacgata tccataacac gacagccacc ggcaaatgcc cgttccatca gggcggtcac
     4501 gaccagagcg caggtggggg cacaaccacg cgcgactggt ggccaaatca actccgtgtt
     4561 gacctgttaa accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc
     4621 aaagaattca gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca
     4681 gaatctcaac cgtggtggcc agccgactgg ggcagctacg ccggtctgtt tattcgtatg
     4741 gcctggcacg gcgcgggtac ttaccgttca atcgacggtc gcggcggcgc gggtcgtggt
     4801 cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt
     4861 cgcctgttgt ggccaatcaa acagaaatat ggccagaaaa tctcctgggc cgacctgttt
     4921 atcctcgcgg gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc
     4981 ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg
     5041 ctgactcacc gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc
     5101 ctgatttacg ttaacccgga aggccctgat cacagcggcg aaccactttc ggcagcagca
     5161 gctatccgcg cgaccttcgg caacatgggc atgaatgacg aagaaaccgt ggcgctgatt
     5221 gcgggtggtc atacgctggg taaaacccac ggtgccggtc cgacatcaaa cgtaggtcct
     5281 gatccagaag cagcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc
     5341 ggcgttggcg cagatgccat tacctctggt ctggaagtgg tctggaccca gacgccgacc
     5401 cagtggagca actatttctt cgagaacctg ttcaagtatg agtgggtaca gacccgcagc
     5461 ccggctggcg caatccagtt tgaagcggta gacgcaccgg aaattatccc ggatccgttt
     5521 gatccgtcga agaaacgtaa accgacgatg ctggtgaccg acctgacgct gcgttttgat
     5581 cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc
     5641 tttgctcgtg cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgttacatc
     5701 gggccggaag taccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac
     5761 aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct
     5821 gttagtgagc tggtatcggt ggcctgggcg tctgcttcta ccttccgtgg tggcgacaaa
     5881 cgtggtggtg ccaacggtgc gcgtctggca ttaatgccgc agcgtgactg ggatgtgaac
     5941 gccgcagccg ttcgtgctct gccagttctg gagaaaattc agaaagagtc tggcaaagct
     6001 tcgctggcgg atatcatcgt gctggcaggt gtggttggcg ttgagaaagc cgcaagcgcc
     6061 gcaggtttga gcattcatgt accgtttgcg ccgggccgcg ttgatgcgcg tcaggatcag
     6121 actgacattg agatgttcga actgctggag ccaattgctg acggtttccg taactatcgc
     6181 gctcgtctgg acgtttccac caccgagtca ctgttgattg ataaagccca gcaactgacg
     6241 ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gcgtactggg tgccaacttc
     6301 gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa tgacttcttc
     6361 gtgaacttgc tggatatgcg ttatgagtgg aaagcgaccg acgaatcgaa agagctgttc
     6421 gaaggccgtg accgcgaaac tggcgaagtg aaatacacag ccagccgtgc ggatctggtc
     6481 tttggttcta actctgtcct gcgtgcggtg gcggaagtct acgccagcag cgatgcccac
     6541 gagaagtttg ttaaagactt cgtggcagcg tgggtgaaag tgatgaacct cgaccgtttc
     6601 gacctgctgt aatctgacct tcttcagcga ctgcctttca tgcagtcgct gaagtttctt
     6661 taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga
     6721 aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc
     6781 tggattttca tgaagcaagt caccagctac atcggtgcct tcgacttcac cgccttacgc
     6841 tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg cggaatgcgc
     6901 ccgacgccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggc
     6961 ctggcacagt gggcgctggt cagcggcgga gcggggaagg tggcaatcct gagctatacc
     7021 atgccgttct gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg
     7081 caatatttcg cgatactgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa
     7141 ctcgatttct cttcgatgaa aagtgccatg ctggcaatcc tctccggtgt gagttggggg
     7201 gcgagcgcga ttgttgctaa acgtctgtat gcccgccatc cgcgcgtgga tttattgtcg
     7261 ctaacatcct ggcagatgct gtatgcggcg ctggtgatga gcgtggtcgc tttactggtg
     7321 ccgcaacgtg aaattgactg gcaacccacc gtgttctggg cgctggccta cagtgcgatt
     7381 ctggcgacag cgctggcgtg gagcttatgg ttgtttgtat tgaaaaactt gcctgccagt
     7441 attgccagct taagcacact ggccgttccc gtttgcggcg tactcttttc ctggtggctg
     7501 ctcggtgaga atccgggggc agttgaaggt agcggtattg tgctgattgt gctggcactg
     7561 gcgctggtga gccgtaagaa aaaagaagcc gtcagtgtaa aaaggatctg aattttttct
     7621 tcatgtgggg agatctctta tttagcaaaa taccgatagt gccccaccat ccgccagcta
     7681 aacagtacat cttcttcctg cgcacctgcg ccaatgttat gtatcaccag cggcgtaccg
     7741 tcgcgggcga agccatctga caccacccca atatgtgcca gcccgttatc taatcgccag
     7801 gagacaatat cgcccgcttg atagtcactg gggttcttgc tggtggggcg tgttttatcg
     7861 tggcgggtaa accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg
     7921 cgctttaact gccatttttg cgggtacgca gcaaaattct tcgccatatc ttcgtgcacc
     7981 agtttctgca aatcgacctt ctggctgcgc aatgcacgga tcaccacatc ggaacatacg
     8041 ccgcgttctt gcgggacatc accgccagga taggtaagct gcacatacgc cggatcgtaa
     8101 aatagcgtgc tgccgatttg ctgtctggcg ccgtctgcga tggcaagatt ggtgttagcc
     8161 tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtaact ggtaaatacc
     8221 gtcagaagac tgaataacac cagtgaagct ttcatctgtt tcgtccgtga aaataagagt
     8281 cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca gaagcggata
     8341 aaaaaacacc cgaacaaggt ggaagtttca gattactggc acagcgtgcc ggatgcggcg
     8401 tgaacgcctt atccggccta caaaagtacg taaatttact atattgcaga gattgcgtag
     8461 gcctgataag cgtagcgcat caggcaattt tgcgttcaaa ctcccggaca agccgggagt
     8521 ttggagtagg ttattcccac tcttgcagga aacgctgacc atactggtcg gctaccagca
     8581 gtgcggcgta aacctgatct ggcgtcgcgc cgccaggcat gttgtggatg gtttcacctt
     8641 ctgcacatgc cgcttctgcc acaattcgca ttttcgccgg gacatcttct ttaatatcca
     8701 gttgagcgag agttattggc aaacctaccg catggctaag cgcagctacg gtttcgattt
     8761 cctccaccgg cgcgttttcc agcaccagct gcgtcagcgt accgaatgct actttttcac
     8821 catgataata gtgatgcgcg tccgggatag cggtcaggcc gttatgtacc gcgtgtgccg
     8881 ccgccagccc gccgctttca aaaccaacgc cactcaaata ggtgttcgct tcaatcacgc
     8941 gctccagcgc cggagtcact acatgctgtt cggcggcaag catcgctttt tcgccttctt
     9001 ccagcagggt gttgtagcac agttcagcca gtgccagcgc tgcctgagtg cacttgccac
     9061 ccgccatggt ggtcgcgccg ctacgggaac aggcacgcgc ttcaaaccag gttgccagcg
     9121 catcgccaat acccgccgct aacagacgcg cgggcgcgcc agcgacgatt ttggtgtcga
     9181 caatgaccat attcgggtta tttggcaaca gcagatagcg gtcaaactca ccctcatcgg
     9241 tgtagataac agacaatgcg ctgcacggtg cgtcggtaga agcgatagtc ggtgcgatcg
     9301 ctaccggaac acccatgaaa tgtgccagtg ctttggcagt atcgagggtt tttccgccac
     9361 cgataccgag aattgcgcca cactgcgcag tctccgcgat gccacgcaga cggtcgatct
     9421 cattttgcga acattcaccg ccaaacggcg caatttctac taccagtcca gcatctttaa
     9481 agcttttctc gacagtggat tgagcaaaac ctaaaacaaa tttgtcaccc accactaacc
     9541 agcgttccgc cagtggcttc aggtactcgc ccagacgatt aattacatct gcgccctgga
     9601 tgtatttacc tggtgattga ataatgcggt ccataattac tcctttagag atgagtagtg
     9661 ccaaatgcgg cattccagtc gtgctcgaac ttctctatag ctgactctac cgcaggggtg
     9721 ttaagcattt gttgcgctac atctaagggc agtgtgatgg attcacatcc agccagcaaa
     9781 caatccagcg cctgacgtgg tgttttaaag ctggcagcca gcaccatgct ttctggcgca
     9841 tgcatttcca gtaacgcttg caactcctgt acagtacgaa tgccgtcacc gccctgggca
     9901 tctacgcggt taacgtatgg agcaacgtat ttggctccag ccagcgccgc cagtaatcct
     9961 tgcgcggcgc tgtacactgc ggttcccagc gtagtaatgc cttctttctt cagcatttta
    10021 attgctgcca gaccttcaga ggttaccgga attttcacca caatgcccgg aatagcgtcg
    10081 cgcaggcgtt tcgcttcttt caccataccc tgcgcgtcgc ggctcatggt ctgagcaaac
    10141 agaattccct catcaccgat cgctttttga aggcgcggca gcacttccca gatggactcc
    10201 ttgctggcag cgataatgct cgggttagtt gtcaccccgg caatcgggaa tatgcgtgcc
    10261 agacgttcga cttctgcgac gttagcggtg tccagataca gttccatgat gtttcctttt
    10321 acagttccag ttcatgttgc agcaggctgg cgatagcgtc ggcagaggcg gcgttaacca
    10381 gcgcattacg gaattcttcg tgcatgatgc ggcgagcgag gcgcgagaaa atgcgcatgt
    10441 gctgatcgcc cgcggcgtgt ttgttcaggg ttaacataat gatgaattgc gcttcatcat
    10501 cgccccagcg caccggcgct tgcaaacgcg ctacgctgat ggtggattgc tcaatgtgtt
    10561 ctgatttgct gtggggaatg gcaaaactaa agcccagacc ggtagagaaa acggcctcgc
    10621 gcgcccacaa gtcggcttcc agtttacgcg gatagcgaca gcggcccgcc agcagcaggt
    10681 tatcggtcat gcctttgagc acttcttctt tgctgcgcca gtcgctttcc agtgtgatgc
    10741 actcggcggt gaccagcggt gcgtcttgtt gggtcatgcg gaattgcgcc agcaggtgtt
    10801 ccacttccag cgaagtacgg caggccattg cctggttgag caacttgcgg cactcacggc
    10861 tatcaagttg cgccatccgc gctttcgccg ccggaattga tggtgcgctc atgctgagtt
    10921 catccagccc taaaccgacc agcaacggca gcacggaacc tttcgctccc agttcaccgc
    10981 acagaccaat ccatttgccc tggcgatgca ccgcctgcac ggcgtaatcg agcgcccgca
    11041 agaaggccgg attcaggctg ttgtagtgac gagtcacctt agcgttatcg cgatccactg
    11101 ccagtagata ttgcgtcagg tcattactac caatactaaa gaaatcaatc tcttcgcagc
    11161 attgatcgat gatgaacatc accgacggca cttccagcat gatgccgagc tggatcttct
    11221 catcaaacgg aatgtgttcg ttacgcagtt gctgtttggc ttccgccagt ttttctttca
    11281 cccataagat ctcttccatt gaggagatca tcgggatcat gattttcagg ctgccgtgag
    11341 cagaggcgcg caggatcgac cgtagttgtg tggtaaacaa cgacgcgtat tcttcataaa
    11401 tacgcaccgc gcgataaccg aggaacgggt tagcttctgc cggaatgttc agataatcaa
    11461 cgggtttatc accgccaatg tccatagtgc gcacaataat gctgcgtccg ttggcagact
    11521 ccagcgcctg acaaaaaatg ttgtacagct cgctttcgcc cggtgcactg gtgcgatcca
    11581 tatagagcat ttcagtgcgg aacaaaccaa cgccttccgc accattgccg aatgcggcct
    11641 gcgcttccac ggagtgagcg atgttagcgg caatttcaat gcggataccg tcagcggtac
    11701 gcgcttgctg ggtcagccag acacgctgtt gctcacgcag agcgtcctgt acgcgggctt
    11761 cttgctgata ataacgggcc acggattccc ctggctcaac cacaatcgcc cctgcgttgc
    11821 cgtcgatata aatcgtctga tgctgccacg gagtaagggc agcaatatcc acacctacca
    11881 gcgtcggaat gttgaacgaa cgggcaagga tcacagtatg tgaggtggtg ccgccacttt
    11941 tcagtaacag acctttgaga tgatttttat ccagttcgag gaactggctg ggggtcagtt
    12001 catccgccat acaaatggcg ggctgcgtca gtttgcccgg tgccgggaag cgttgctcac
    12061 cgtagatttg ctggagtaac tggaagcaga cgtcgcgtac gtccagggca cgttcttgca
    12121 ggtagctgct gctggaacgg gcgaactctt cgcaaaagtg attcgcactg gcaacaatcg
    12181 cttcggcgca gcttaatccg gcgctgacac ctgccagtaa atgttcgcgc agggaagtat
    12241 cgccagccag ggatcggtgc gcttccagaa tcgcgctggt agcaccgtcg ctatccagca
    12301 gacgaaactc tatgtttttc agtaccagcg tcagaccgtt ttccagtgcg gattgttcgg
    12361 cgtcaacgcc tttggctgcg ggaagattac ccagcgcatt gagatctaaa gaagagatcg
    12421 gcgtcagaat gccgcctgcg ctaccactgc acacggtgcg agcacggata atttgcggat
    12481 tcagattggt cagtgaaacc ggcagtggtt ccagttcgtc agattttact tccgccagcg
    12541 gtgcgtcgca gtgggggaat tcatcgcgca gccattggct taaacgctgg tacgcttcct
    12601 gttcgtcggc cccggaaatc agtaactggc agttatcgcc caccagcgta tcggtgccaa
    12661 tcagcgcaag ggcgcttttg gcgttgccct tgcggtcagt gcgcaggtta tgccactcaa
    12721 tttgtgatga aaaagtatta cacagcgttt caacgtggct tgccggacgc gcatgcacgc
    12781 cgttaggtag ctcacaaata aattccacaa tcagggccat tgcctttctc ccggtacggt
    12841 tttctgttta caggataaga gcgcacggca acggcctgcc atgtgacaaa tctgccaaaa
    12901 gctggacaaa tgtaatgtaa ccgtcaattt gcgacgcgtc tcacaagacg ccgttttgcg
    12961 gcatgcttcc ggattatcgc aagttatgag gcggatcgca tttttgtact gatattacaa
    13021 aagtccagta aatggccttt ttatccactg tttgctccgc ctgcgattgc ctattgtttc
    13081 ttccatcaaa aatatggaca tgggctttcg caagcccggg agcagcttat gaatgagttg
    13141 gtgcagatcc tgaaaaatac ccgtcagcat ttaatgacgg gcgtttcaca catgattccc
    13201 ttcgtggtat cgggcggtat tttgctggcg gtttccgtca tgttgtatgg caaaggcgca
    13261 gtgccggatg ccgtagccga tccgaatctg aaaaaactgt ttgatatcgg cgttgcaggt
    13321 ttgacgctga tggtgccttt cctcgccgct tacatcggtt attccattgc agaacgttct
    13381 gcgctggctc catgcgctat cggtgcctgg gttggtaaca gctttggcgc gggcttcttt
    13441 ggtgcgctga tcgccgggat tatcggcggc atcgtggtgc attacctgaa gaaaattccg
    13501 gtgcataaag ttctgcgctc ggtgatgcca atcttcatca ttccaatcgt cggcacactg
    13561 attaccgctg gcgtcatgat gtgggggctg ggcgagccgg tgggggcgtt gaccaacagc
    13621 ctgacccagt ggcttcaggg gatgcagcag ggcagcattg ttatgctggc ggtgatcatg
    13681 ggtctgatgc tggcgttcga tatgggcggc ccggttaaca aagtggccta tgccttcatg
    13741 ctgatttgcg ttgctcaggg tgtttatacc gtggtggcta ttgctgccgt ggggatttgt
    13801 gttccaccgc tggggatggg gctggcgacg ctgattggtc gtaaaaattt ctccgcagaa
    13861 gagcgcgaaa ccggcaaggc ggcactggtg atgggctgtg ttggcgttac tgaaggggcg
    13921 attcctttcg ccgctgccga tccgctgcgt gtcattcctt ccatcatggt cggttcagtt
    13981 tgtggtgcgg taactgcggc gctggtcggt gcgcagtgct atgcaggctg gggtggtctg
    14041 attgtgctgc cggtggttga aggtaagttg ggttacatcg cggcagtggc tgtcggggcg
    14101 gttgtgacgg cagtctgcgt gaacgtgctg aaaagtctgg cgcgtaaaaa tgggtcttca
    14161 actgaagaga aggaagacga cctggatctg gattttgaaa ttaattaatt gaggaacgag
    14221 ccatgacgaa aattatcgca gtaaccgcat gtccttcagg tgttgcccat acttatatgg
    14281 cggcagaggc gctggaaagc gccgcgaaag cgaaaggctg ggaagtgaaa gtagaaaccc
    14341 aggggtcgat tggtctggaa aatgaactga ctgcggaaga cgtggcaagc gccgatatgg
    14401 tgattttgac caaagatatc ggcatcaaat ttgaagagcg ttttgctggc aagactatcg
    14461 tgcgtgtcaa catcagcgac gcggtaaagc gtgctgacgc cattatgagc aaaattgaag
    14521 ctcacctggc acaaaccgct taagttctta tcccgcccgc aagggcgggt tcgctttccc
    14581 acaggagttc ctcatgacga atcgtatctc tcgcctcaaa actgcactgt ttgccaatac
    14641 ccgtgaaatc tcgctggagc gggcgttgct ttataccgcc agccatcggc aaaccgaagg
    14701 ggagccggtg ataatgcgtc gggcgaaagc aacagcgtat atccttgaac acgttgaaat
    14761 ttcgattcgt gatgaagaac tgattgccgg taaccgcacc gtaaaaccac gcgccgggat
    14821 tatgtcgccg gaaatggacc cttactggct gctgaaagaa ctggatcaat tcccgacgcg
    14881 tccgcaggac cgctttgcta tcagcgaaga agataaacgt atctaccgcg aagagttgtt
    14941 cccgtactgg gaaaaacgtt cgatgaaaga tttcatcaac gggcagatga cggatgaagt
    15001 aaaagccgcg accagcacgc agattttcag catcaaccag acggataaag gccaggggca
    15061 cattattatt gattacccgc gcctgctgaa tcacgggctg ggggagctgg tggcacagat
    15121 gcagcaacat tgtcagcaac agccggagaa tcacttttat caggcaacgc tgttactgct
    15181 ggaagcctcg cagaaacata ttttgcgtta cgccgaactg gcggaaacga tggcggcaag
    15241 ctgcactgat ggcccgcgtc gcgaagaact gctgaccatt gcggagatct cccgccataa
    15301 cgccgaacat aagccgcaga cgttctggca ggcgtgccag ttattctggt acatgaacat
    15361 cattctgcaa tacgaatcca acgccagttc gctatcgttg gggcgcttcg accagtatat
    15421 gttgccgttc tatcaggcat cattaaccca gggcgaagat ccggcattcc tgaaagaact
    15481 gctcgaatct ttatgggtga aatgcaacga catcgtgctg ttgcgctcca ccagtagcgc
    15541 gcgttatttc gcaggtttcc ctaccggcta taccgcactg ctcggcgggt taaccgagaa
    15601 cggacgtagc gcggtgaacg tgctttcgtt cctttgcctt gacgcttatc aaagcgtgca
    15661 attaccgcaa ccgaacctcg gcgtgcgcac taacgccttg atcgacacgc cgttcctgat
    15721 gaaaaccgca gaaaccattc gcctcggcac cggtattccg caaatcttta acgatgaagt
    15781 ggtggtgcca gcgttcctca accgtggcgt ttcgctggaa gatgcgcgcg actattccgt
    15841 agtgggctgt gtggaattat ctattcccgg cagaacctac ggcttgcatg acatcgcgat
    15901 gtttaacctg ctgaaagtga tggaaatctg cctgcatgaa aatgaaggca acgctgcgct
    15961 gacttatgaa ggtttactgg aacagatccg cgccaagatc agccactaca tcaccctgat
    16021 ggttgagggc agcaatattt gtgatatcgg ccatcgcgac tgggcacctg taccgctact
    16081 ctcgtctttt atcagcgatt gtctggaaaa aggccgcgat attactgacg gcggcgcgcg
    16141 ttataacttc tccggcgtac aggggatcgg tatcgccaac ctgagcgatt ctctccatgc
    16201 gttgaaaggg atgatttttg atcaacagcg tttaagtttt gacgaattgc tgtcggtatt
    16261 aaaagccaac ttcgcaacgc cagaaggcga aaaagtccgc gctcgcttaa ttaaccgctt
    16321 cgagaaatac ggtaacgata tcgacgaggt ggataacatc agcgccgaac tgttacgcca
    16381 ctactgcaaa gaagtggaaa aataccagaa cccgcgcggc ggctacttca cgccaggatc
    16441 gtataccgtt tctgctcacg tcccgttggg atcggtggtt ggcgcgacgc cagacggtcg
    16501 ttttgccgga gaacagctgg cagacggcgg cttgtcaccc atgctgggcc aggacgcaca
    16561 agggccaaca gcggtactga agtcagtcag taagctcgat aatacgctgc tgtctaacgg
    16621 tacgttgctg aacgtgaaat tcactccggc gaccctggaa ggtgaagcgg gattacgcaa
    16681 actggccgac ttcttacggg cgtttaccca gcttaagttg cagcatattc agtttaacgt
    16741 ggtgaacgcc gacacgttgc gggaagcgca acagcgccca caagattatg ccgggctggt
    16801 ggtgcgcgtt gccggataca gcgccttctt tgtcgaactg tcgaaggaga tccaggatga
    16861 catcatccgc cggacagcgc atcagctgta acgttgtgga aacgcgccgc aatgatgtgg
    16921 cgcgcatttt caacattcag cgttattcac tgaatgacgg cgagggcatt cgtacggtgg
    16981 tcttttttaa aggctgtcca catctttgcc cgtggtgtgc taatccggag tcgatctccg
    17041 gcaaaatcca gactgtacgc agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg
    17101 atgcggatga atgcccctcc ggtgcgtttg aacggattgg tcgcgatatc agccttgacg
    17161 ctctggaacg ggaagtgatg aaagatgaca ttttctttcg cacgtccggc ggtggcgtca
    17221 cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc gacccgtttt ttacagcgac
    17281 tgcggctatg gggtgtgtca tgcgccatcg aaactgccgg agacgcgcca gccagcaagc
    17341 tgttaccgct ggcgaaattg tgcgatgaag tgttgttcga tttaaaaatt atggatgcgg
    17401 ctcaggctcg ggatgtggtg aagatgaacc tgcaacgcgt gctggagaat ctgcgtttgc
    17461 tggtgagtga gggtgtcaac gtgatcccgc gtttaccgtt gatccctggt ttcacgctca
    17521 gccgggagaa tatgcagcag gcgctggacg tgctgatccc gctgaatatc aggcagatcc
    17581 atctgttacc gtttcatcag tacggcgagc cgaaataccg cctgctgggg aaaacatggt
    17641 cgatgaaaga ggttgctgcg ccgtcgtcag ccgatgtggc aacgatgcgt gaaatggcag
    17701 aacgggccgg atttcaggtt accgtgggag gttaaaatgg catatctggt ggcagtaacc
    17761 gcctgcgtaa gcggcgtggc gcatacttat atggcggcgg aacggctgga aaagttgtgc
    17821 cagttagaga agtggggcgt tagcattgaa actcagggcg cgttgggaac ggagaatcgt
    17881 ttagcggacg aggatatccg ccgggcggat gttgcgctgt taattacgga tatcgagctt
    17941 gccggtgctg agcgatttga acattgtcgc tacgtgcaat gcagcatcta cgctttcctg
    18001 cgtgagccgc agagggtgat gagcgcagtg cgcaaagtgc tttctgcgcc gcagcaaacc
    18061 catcttattc tggagtagtc ggtttttctg tcagttggct gtggtactgc cgacgatact
    18121 ccgacggcga gcgttcggta tttttacgaa acagacggca aaagtagttg ctgtcgacaa
    18181 agccgcaggc gtgcgccact tcttttacct tcaggtcata gcctttcaat aatgtcttag
    18241 cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc gttttttgaa
    18301 acaggtgcga gaggtaattt ggcgaaatat agaacgcctg tgcgacagat tcgcgggtaa
    18361 gtgcggaggc atagcgttcg tcgatataat cacgaatagc ttcgaataga gcctggctgc
    18421 gtgaggcggt ctgtatttgg ctgccgagca aatcgcggca gtggctaagc aggctggtga
    18481 cgataagtct ggccgtttgc tgctcctgcg actgcatttg catttcattg agcgtttgta
    18541 gcagaaaaga accaatgcgc gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat
    18601 attgtttgcc atcccattgc acaacgctga agccgagttg ctgtttgcca aacagcacgc
    18661 taaaggtggt ggcgggggct tgccattgtg gaaaattcca gccaccagca gggacgtaca
    18721 gcacatcgcc gggaactaac gctgctccag cgccggtatc aataaactca ccttccagca
    18781 caatttccag ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg ccgttgctgc
    18841 tggcaaagta aatctggcgc agggacagcg ggccgttgat caggcgggag agcagatagc
    18901 tgacgtcgtg atacatgtta ttcccttagg tatgtagacg ggaaaacagt aactggcatt
    18961 atgaaaaaag gccacctgaa aagtgtggcc tgatgggagt gagatgtaaa cattgcggca
    19021 gtttcagtcg acttgtattg ccggatgcgg cgtaaacgcc ttatccggcc taccgccgcg
    19081 atctgtaggc cggataagac gcgtcaagcg tcgcatccgg caaataacat ctggctgata
    19141 attactgatt acccacctga tcgccatacg gcagagtgtc gtaatcgatc agtgcgtttt
    19201 tcttatacgg gttaccaatc cagcgggtag tttctttgaa ctgcggattc accactgaac
    19261 gggttggatc gtaaccgtcg taagataagc ccgccaaatc tgaccaggtg tggatcagtt
    19321 ccgccaggct gtatttacga tcaacatcct gtgagaaatc acgcggatga gtcgcttgcc
    19381 atttttctga cgtccacagc aggaacggaa tggtgtacat atgacgcgtc gggttgtctt
    19441 cattacgccc ctgagttttg tgcggcggcg tgtcgtaaac ctcttcaccg tggtcagaga
    19501 aataaaccag gaaaccgttc ggatcggtcg ctttaaagtc tttaatcagg ctggcaacca
    19561 cgtgatcgtt gtacaggtta gcattgtcat aatcattata tgactccagc tcttctgcgt
    19621 ttaatcccgg tggaacatgc tctgtattac cgtcaaattt gccctgatct tccggataac
    19681 ggtacttgta cttgatatgc gtacccagca gatgaacgat gatcagtttc ttcggcgcag
    19741 ggtccttcag cacttcctgg aacggcttca gcacgttggt gtcgtattca cgcgcactct
    19801 gcgtacgttg ctggttcatg tagtactgct tgtcggtctg gcgcgaaaat accgtcagca
    19861 tggtattgcg ggcggtcatc gtctgctggt tggtgatcca gaaggtttta taacccgcct
    19921 gtttcatcat gttcatcagc gacggctgcg tcagatacag atccgggttc ttttcattgg
    19981 caaaggtcag cgcctgttgc aggatttcaa tggtgtacgg acgagacgta accacgttat
    20041 taaacacggt cagattcgga tcggttttat gcagcgcatc cagctccggc gtggtttcac
    20101 gcggataacc gtacagactc atgcgtccac gctgggtcga ttcgccaatt accagcacta
    20161 aggtgcgcgg ttcgttaccc gattcatctt tgaaattagc cagcggcggc aaggcattat
    20221 tttcattcag tagctttgtt agcgagttta gttgctgacg atactgataa tagccggtca
    20281 ggaattgcca cggtgcggca ggctccatac gcgatgccag gttatccaac gttttctcaa
    20341 acggcttgtt cttgataaac gtattcatgg cgatcggatg cagaatcaag ccataaagca
    20401 gggcaaaaga gacaacataa cgccacggct ttggaatata gaccgggcgc aggcgtgtcc
    20461 acagcagaac tgccaccgcc gtataggcca gcgcgataag cacaattttc aggctgaaat
    20521 actggcttaa atactcgctg gcttcgttag tgttggtttc gaacatcaca aacagaacgc
    20581 tctgcgagaa ctcctgaccg tagatgacgt agtagcacag cgccgccaga gaggccgccc
    20641 atagcaccac gccgatcact gcggcaataa ttttaatccg cttcggaaag aggaataccg
    20701 ggatcaacca cagcgaactg aataacagcg agtcgcgaat gccgttagtg ccactataac
    20761 cactgatgta aataatggcc tgtagcagag tagagaaaaa ccaaaagtag agcagtgccc
    20821 aacccagggc tttccagcta aaaaggggtt tagcctggac ttctgtggaa tgcatagtaa
    20881 gaacctgtct tgaaaaaaat atcgccgaat gtaacgacaa ttccttaagg atatctgaag
    20941 ggatatccag attttgaata aaatgcagac agaaatatat tgaaaacgag ggtgttagaa
    21001 cagaagtatt tcagaaaacc ctcgcgcaaa agcacgaggg tttgcaggag aggaagatta
    21061 gccggtatta cgcatacctg ccgcaatccc ggcaatagtg accattaacg cctgctcgac
    21121 gcgaggatcc ggttcctggc cttctttttc tgcctggcgg gagcggtgca gcaactcggc
    21181 ctgcaatacg ttcagcgggt cggtgtaaat attccgtagc tgaatagact ctgcaatcca
    21241 cggcagatcg gccatcaggt gagaatcgtt ggcaatcgcc agcaccactt tgatgtcttc
    21301 ttcttgcaga ttgcgtaact ctttacctaa cggccacagt gctttgtcga ccaggcgttg
    21361 gtcatagtat tccgccagcc acaggtctgc tttggcgaag accatctcca gcatgccgag
    21421 acgcgtcgag aagaatggcc aatcgcggca catggcttcc agctcgctct gtttgccatc
    21481 ttcgaccact ttttgcagcg ccgtacctgc acccagccag gcggggagca tcaggcggtt
    21541 ttgcgtccag gcgaaaatcc acggaatagc gcgcagtgac tcgacgccgc cggttgggcg
    21601 acgtttcgcc ggacgtgaac ccaacggcag tttacccaat tcttgttccg gcgtagcgga
    21661 gcggaagtaa ggcacaaaat ctttgttttc acgtacgtag ccgcggtaga catcgcaaga
    21721 gataaccgaa agctcatcca taatgcgacg ccagctctct ttcggctctg gcggtggcag
    21781 caggttggct tccagaatcg ccccggtata cagcgaaagg ctgctaacgg tgatttcagg
    21841 cagaccgtac ttgaagcgga tcatctcgcc ctgttcggtc acgcgcaggc cgcctttcag
    21901 gcttcctggt ggttgtgaca gcagcgccgc atgggcaggt gcgccgccgc gaccaatgga
    21961 accgccgcga ccgtggaaca acgttagctc aatacccgct ttttcgcagg ttttgattaa
    22021 tgcatcctgt gcctgatatt gcgcccagga agctgccatc acgcccgcat cttttgctga
    22081 gtcggaatag ccaatcatca ccatctgttt gccctgaatc aggccgcgat accagtcgat
    22141 attcagcaac tgggtcatga catcgttggc gttgttcagg tcatcgaggg tttcaaacag
    22201 cggagcaacc ggcatcgcaa acccgatacc cgcttctttc agcagcaggt ggacagccag
    22261 cacgtcggac ggcgttttcg ccatcgagat cacgtaggcg gcaatggagc cttgcggcgc
    22321 ttcggcaatc acctggcagg tatcgagcac ttcacgcgtt tcggcgcttg gttgccagtt
    22381 gcgtggtaga agcggacgtt tggagttcag ttcgcggatc aggaacgcct gtttgtcggc
    22441 ttctgaccag ctttcataat cgccgatacc gaggtagcgg gtcagttcgc ctaatgcttc
    22501 ggtatgacgc gtgctctcct gacggatatc aatacgcacc agcggtacgc cgaaacattt
    22561 cacgcggcgc agggtgtcga gcagatcgcc attggcgata atacccatgc cacacgcctg
    22621 aagtgactgg tagcaagcgt agagcggttc ccacagttct tcgttttgtg tcagcagacc
    22681 ttctggtttt ggcaattctt cgcctttcag gcgcgcttcc agccatgcct gtgtcgccat
    22741 caggcgagaa cgcaggtttt tcatcagata gcgatacggt tctgcggcac cctcttcgcc
    22801 aaccagcgcc agcagttcag gggtggcttc aaccatcgac agttcagaaa ccagaacctg
    22861 aatatctttc aggaacaaat cggtggcttt ccagcggctg agtagcagga cgtggcgggt
    22921 gatatcggca gtgacgttcg ggttgccgtc gcggtcaccg cccatccacg aggtaaaacg
    22981 gaccggaaca aattcgacgg gcagtttgta gccgaggttc tcttccagtt gttcgttcag
    23041 ttcgcgcagg taattcggta cgccttgcca caggctgttt tctactacgg caaagcccca
    23101 tttggcttca tctaccgggc ttggacgcag cttacggatt tcatctgtat gccatgactg
    23161 ggcgatcaac tggcgcaggc ggcgcatcag ctggttatgt tcgtagtcgg cgatatcttt
    23221 gttatcgagc tgttttaagc aggcgttcac ttccaccatt ttgtggatca gtgtacgacg
    23281 ggtaatttcg gttgggtgag ccgtgaggac cagttccagc gacagcgatt ccactgcttt
    23341 ttttatggtg tcttcgctca gttccggctg gtttttcagt ttgcgcaggg tgcgggcgat
    23401 cacttccggg ttgctggcag cttcgccttt cggcgaaatg ctgtggtatt gctcggcggt
    23461 gttggccagg ttcagaaact gactaaacgc acgcgcaacg ggcagcagct cgtcgttcga
    23521 caaattttgt aaggtggtga gcaactcctg gcggttagca tcattgccag cgcgtgaaga
    23581 tttggacaac ttacggatag tttctacgcg ttcaagaatg tgttctccca acgcatcctt
    23641 gatggtttct cccagcactt tgccgagcat actgacatta ctacgcaatg cggaatattg
    23701 ttcgttcata ttaccccaga caccccatct tatcgtttga tagccctgta tccttcacgt
    23761 cgcattggcg cgaatatgct cgggctttgc ttttcgtcgt cttttataaa gccacgtaaa
    23821 agcggtgacg tcaaatgctg cgaaatcgct tcagcaaacg aataaatagc aggaatttac
    23881 gccattaaat tcacgacgct ttaaataagc gtaacttatg ggaatgttaa aaaatcgcct
    23941 caattaacac caaaggtgta ggtcggataa gatgcgtaag catcgcatcc gacattgttt
    24001 ggcctacgtt ttaatgccag caaaaatggt gaatcacctg ggtgatcagt tcgcgggtgg
    24061 gcttgataaa cctcgtctcc agatattcat caggctgatg agcctgatta attgagccag
    24121 gccccagaac cagcgtcggg cataacgttt gaataaacgg cgcttcggta cagtaattca
    24181 ccacttcggt ctttgctccg agcaatttct caaccacttc aaccagttga tgattcggtg
    24241 ggcattcata gccagggatc ggcggatgca gctcgtcgac cgtcagacga cccggccagc
    24301 gttcgctcac tggagccaat gcatcgttga gcaaaccgtt aagttcattg agtgtcatgc
    24361 ctggcagcgg acgaatatcc atatgcaact cacaacaagc gcaaatacgg ttagatgcat
    24421 cgccgccgtg gatatgcccg aggttgagcg tagggtatgg cacggtaaac gcgtcgtagt
    24481 gataacgttc tttcaggtta tcgcgcaatt gcaaaatatg cccgatggcg tcgtgcatca
    24541 gttcgatagc gttaactccg cgtgctggat cgctggagtg gcccgactgg ccctgaatac
    24601 ggatggcgtt agagatatga cctttatgcg cgcgtaccgg ttgcagcgac gttggctcgc
    24661 caatgatggc gcaatccggg cgcagggcgg tagtttcggc aaaataacgc gctccggcca
    24721 tactggtttc ttcatcggca gtcgccagaa tgtagagcgg ttttgcaagc ttcgtgacgt
    24781 cgacatcgcg tagcgcatca aggataaacg caaaaaagcc tttcatgtcg gcggtgccta
    24841 agccgtaaag cttgccgtca tgctccgtca acgtaaatgg atcgcgcgtc cagcgtccgt
    24901 catcaaatgg caccgtatcg gtatgccccg ccagcaacaa accgccagcc ccctgtccgc
    24961 aactggccag catattaaat ttgttgcgag ttcctggcac aggctgtact tccacattga
    25021 agcctaaatc cttaaaccag tccgccagca gagtgattaa atctgcattg ctttgatcga
    25081 gtgcctcttc cgtggcgctt attgaaggtg tggcaatcag agcacggtaa atctcgataa
    25141 atggcggtaa tttgtttttc attgttgaca cacctctggt catgatagta tcaatattca
    25201 tgcagtattt atgaataaaa atacactaac gttgagcgta ataaaaccca ccagccgtaa
    25261 ggtgaatgtt ttacgtttaa cctggcaacc agacataaga aggtgaatag ccccgatgtt
    25321 gaatacgctg attgtgggtg ccagcggcta cgctggcgca gagctagtga cctatgtaaa
    25381 tcgccatccg catatgaaca taaccgcttt gactgtttca gcgcaaagca atgatgcggg
    25441 aaagttaatc tccgatttgc atccgcagct aaaaggcatc gttgatctgc cgttgcagcc
    25501 gatgtcggat atcagcgagt ttagcccagg ggtggacgtg gtgtttctcg ccaccgccca
    25561 tgaagtcagc cacgatttag cgccgcaatt tcttgaagca ggctgtgtgg tgttcgacct
    25621 ttccggcgcg tttcgtgtta acgatgtcgc cttctatgaa aaatattacg gctttaccca
    25681 tcaatacccg gaactgctgg aacaggcagc ctacggtctg gcggagtggt gcggtaataa
    25741 attaaaagaa gcgaacttga ttgccgtgcc aggctgttat ccgacggcag cacagctggc
    25801 gctgaaaccg ttgattgatg ccgatcttct tgacctcaat cagtggccgg tgatcaacgc
    25861 caccagcggc gtgagcggtg caggacgtaa ggcagcgatt tcaaatagtt tttgtgaagt
    25921 tagcctgcaa ccgtatggcg tctttaccca tcgccatcaa ccagagatcg ccacacacct
    25981 cggtgctgac gttatcttca ccccgcatct gggcaatttc ccgcgcggca ttctcgaaac
    26041 cattacctgc cgcctgaaac cgggtgtgag ccaggtgcaa gtggcgcaag cgttacagca
    26101 ggcgtatgcc cataaaccac tggtgcggct gtatgacaaa ggcgttccgg cgctgaaaaa
    26161 tgtcgttggg ctgccatttt gcgatatcgg gtttgccgtt cagggcgagc atctgattat
    26221 tgtggcgacc gaagacaact tactgaaagg cgcggcggca caagcggtac agtgcgccaa
    26281 tattcgtttc ggctatgcgg aaacgcagtc tcttatttaa gggtgcaatg atgaatccat
    26341 taattatcaa actgggcggc gtactgctgg atagcgaaga ggcgctggaa cgtctgttta
    26401 gcgccctggt gaattatcgc gagtcacatc agcgtccgct ggtgattgtc cacggtggcg
    26461 gttgcgtggt ggatgagctg atgaaagggc tgaatctgcc ggtgaaaaag aaaaatggcc
    26521 tgcgggtgac gcctgcggat cagatagaca ttatcaccgg agcactggcg ggaacggcaa
    26581 ataaaaccct gctggcatgg gcgaagaaac atcagattgc ggccgtaggt ttgtttctcg
    26641 gtgacggcga cagcgtcaaa gtgacccagc ttgatgaaga gttaggccat gttggactgg
    26701 cgcagccagg ttcgcctaag cttatcaaca gcttgctgga gaacggttat ctgccggtgg
    26761 tcagctccat tggcgtaaca aacgaagggc aactgatgaa cgtcaatgcc gaccaggcgg
    26821 cgacggcgct ggcggcaacg ctgggcgcgg atctgatttt gctctctgac gtcagcggca
    26881 ttctcgacgg caaagggcaa cgtattgccg aaatgaccgc cgcgaaagca gaacaactga
    26941 ttgagcaggg cattattact gacggcatga ttgtgaaagt gaacgcggcg ctggatgctg
    27001 cgcgcacgtt gggccgtccg gtagatatcg cctcctggcg tcatgcggaa cagcttccgg
    27061 cactgtttaa cggtatgccg atgggtacgc ggattttagc ttaagttttg ttggccggag
    27121 gcgcagcttt ccggcattga atttcaaaat aaggaaacag agttatggca ctttggggcg
    27181 ggcgttttac ccaggcagca gatcaacggt tcaaacaatt caacgactca ctgcgctttg
    27241 attaccgtct ggcggagcag gatattgttg gctctgtggc ctggtccaaa gccctggtaa
    27301 cggtcggcgt gttaaccgca gaagagcagg cgcaactgga agaggcgctg aacgtgctgc
    27361 tggaagatgt tcgcgccagg ccacaacaaa tccttgaaag cgacgccgaa gatatccata
    27421 gctgggtgga aggcaaactg atcgacaaag tgggtcagtt aggcaaaaaa ctgcataccg
    27481 ggcgtagccg taatgatcag gtagcaactg acctgaaact gtggtgcaaa gataccgtta
    27541 gcgagttgct gacggctaac cggcagttac aatccgctct ggtcgagacc gcgcagaaca
    27601 atcaggacgc ggtgatgccg ggttacactc acctgcaacg cgcccagccg gtgacgttcg
    27661 ctcactggtg cctggcctac gttgagatgc tggcgcgtga tgaaagccgt ttgcaggacg
    27721 cgcttaagcg tctggatgtc agcccgctag gctgtggcgc actggcagga acggcctatg
    27781 aaatcgaccg tgaacagtta gcaggctggc tgggctttgc ttcggcgacc cgtaacagtc
    27841 tcgacagcgt ttctgaccgt gaccatgtgt tggaactgct ttctgctgcc gctatcggca
    27901 tggtgcatct gtcgcgtttt gctgaagatc tgattttctt taacaccggc gaagcggggt
    27961 ttgtggagct ttctgaccgt gtgacttccg gttcatcatt aatgccgcag aagaaaaacc
    28021 cggatgcgct ggagctgatt cgcggtaaat gcggtcgggt gcagggggcg ttaaccggca
    28081 tgatgatgac gctgaaaggt ttgccgctgg cttacaacaa agatatgcag gaagacaaag
    28141 aaggtctgtt cgacgcgctc gatacctggc tggactgcct gcatatggcg gcgctggtgc
    28201 tggacggcat tcaggtgaaa cgtccacgtt gccaggaagc ggctcagcag ggttacgcca
    28261 acgccaccga actggcggat tacctggtgg cgaaaggcgt acctttccgc gaggcgcacc
    28321 atattgtggg tgaagcggtg gtggaagcca ttcgtcaggg caaaccgctg gaagatctgc
    28381 cactcagtga gttgcagaaa ttcagtcagg tgattggcga agatgtctat ccgattctgt
    28441 cgctgcaatc gtgcctcgac aagcgtgcgg caaaaggcgg cgtctcaccg cagcaggtgg
    28501 cgcaggcgat tgcttttgcg caggctcggt tagagtaatt tgtttatatt gctcccctga
    28561 aaatggggag cttaccacgc ctataaagta gcgacactac gtaggcggac tgcctcaggc
    28621 agaaatttgt cagtattctg gtgaccttaa atttaacgcc caaccagaac cattatttgt
    28681 cacattcctt tttattcttg cgtcttcttt accttcactc tccccataaa aattatattt
    28741 tcaatacatc aa
//
LOCUS       DAZKHK010000063        28235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKHK010000063 DAZKHK010000000
VERSION     DAZKHK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.063"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..456)
                     /gene="tssE"
                     /locus_tag="ACHOS5_002733"
     CDS             complement(7..456)
                     /gene="tssE"
                     /locus_tag="ACHOS5_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9777525.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSEEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTSILQGMPGTAHQLMCVLSDVLLKYEPRIKRVDVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            complement(459..995)
                     /gene="tssJ"
                     /locus_tag="ACHOS5_002734"
     CDS             complement(459..995)
                     /gene="tssJ"
                     /locus_tag="ACHOS5_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9777526.1"
                     /translation="MAIIAGKTGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWLQPGGSVAVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(976..2076)
                     /gene="tssG"
                     /locus_tag="ACHOS5_002735"
     CDS             complement(976..2076)
                     /gene="tssG"
                     /locus_tag="ACHOS5_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9777527.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYTGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYPGSRLDVRLQLCVKRSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTISLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(2031..3794)
                     /gene="tssF"
                     /locus_tag="ACHOS5_002736"
     CDS             complement(2031..3794)
                     /gene="tssF"
                     /locus_tag="ACHOS5_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9777528.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KIAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVENVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENVSLPAGISGFDLE
                     VVLSQPWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVTGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEVDRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVRNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(3802..4494)
                     /locus_tag="ACHOS5_002737"
     CDS             complement(3802..4494)
                     /locus_tag="ACHOS5_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP9777529.1"
                     /translation="MSVMHYLQDKTFQDIVKNLVLPLLSGIIIPLVKWFVQHYGYAPN
                     IRKYRFEKIPVSEKESILARIDKLTKEPTTKNTLVRIKYCYEQMGIYLPIWCCNKLIC
                     FISDRNVSSVDNRLHCFLKYSFVGIFSDGKFTVNTRRVHKGYRMIAVFAVFSLCVQFT
                     GGIFTTMPFLSGGNTVLFMLFSLVYFIMIFLTVIFTCNLINEIRLAVQFGRLFEAWLK
                     SERESPEQLALF"
     gene            complement(4496..6094)
                     /gene="tssA"
                     /locus_tag="ACHOS5_002738"
     CDS             complement(4496..6094)
                     /gene="tssA"
                     /locus_tag="ACHOS5_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9777530.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADEVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLAELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNPGSS"
     gene            complement(6094..9483)
                     /locus_tag="ACHOS5_002739"
     CDS             complement(6094..9483)
                     /locus_tag="ACHOS5_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP9777531.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDI
                     RHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
                     NADWLAALRRLRYRPVDGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLTQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPAIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVTVPESRLHGPLATTFGPVLALMDNQNNSADLLNLQTYLTRITQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLAAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNTRWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLRREGNRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPEMVFELSGTSAFTGNDEDAGDSVEETD"
     gene            complement(9476..10624)
                     /locus_tag="ACHOS5_002740"
     CDS             complement(9476..10624)
                     /locus_tag="ACHOS5_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP9777532.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGTLLFIIRASGY
                     VKVLNDFSIWWLALTPSGCWFFLFCLRHWQWSNQMDEHLFLKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLMLVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(10628..10894)
                     /locus_tag="ACHOS5_002741"
     CDS             complement(10628..10894)
                     /locus_tag="ACHOS5_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP9777533.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(10926..11603)
                     /gene="tli1"
                     /locus_tag="ACHOS5_002742"
     CDS             complement(10926..11603)
                     /gene="tli1"
                     /locus_tag="ACHOS5_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFP9777534.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDTTRPSQVSVGRWNEHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKSDFTYGYDEDIKEFIKDK
                     TYPYGNW"
     gene            complement(11623..13305)
                     /gene="tle1"
                     /locus_tag="ACHOS5_002743"
     CDS             complement(11623..13305)
                     /gene="tle1"
                     /locus_tag="ACHOS5_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFP9777535.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(13302..15821)
                     /locus_tag="ACHOS5_002744"
     CDS             complement(13302..15821)
                     /locus_tag="ACHOS5_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9777536.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEVVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKVTGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLELALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(15900..16127)
                     /locus_tag="ACHOS5_002745"
     CDS             complement(15900..16127)
                     /locus_tag="ACHOS5_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777537.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            complement(16124..16765)
                     /locus_tag="ACHOS5_002746"
     CDS             complement(16124..16765)
                     /locus_tag="ACHOS5_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777538.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(16752..19439)
                     /gene="tssH"
                     /locus_tag="ACHOS5_002747"
                     /gene_synonym="clpV"
     CDS             complement(16752..19439)
                     /gene="tssH"
                     /locus_tag="ACHOS5_002747"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9777539.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSHSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVRVDEPDDETACLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENELMTARDDLEQQYARECE
                     LTGELLESRSDISRQSETHHLQQALHDIQQNQPLLSVDVDVRTVAGVVADRTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALESIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLSEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRTIVEMKLA
                     QVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYL
                     AARQHPTYLSLTWNDEDGIVIAFDGINSDYPQESIYGQC"
     gene            complement(19608..20099)
                     /gene="hcp"
                     /locus_tag="ACHOS5_002748"
     CDS             complement(19608..20099)
                     /gene="hcp"
                     /locus_tag="ACHOS5_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFP9777540.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            complement(20134..21834)
                     /locus_tag="ACHOS5_002749"
     CDS             complement(20134..21834)
                     /locus_tag="ACHOS5_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9777541.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVVAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLQTCDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNIWQQQLTSLTALPPSAGVSSQVLPLPDILLSGIPRHRGLSHWHRLCRQTGLIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSELKTTALQRLQADSRLLNDWQ
                     QHGVPQRYGLGLYQGMWLIPPVERATGEWVAPLPPRPVIQKVTQTPKTVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQVDACRLPDITRAP
                     "
     gene            complement(21837..22490)
                     /gene="tssL"
                     /locus_tag="ACHOS5_002750"
     CDS             complement(21837..22490)
                     /gene="tssL"
                     /locus_tag="ACHOS5_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP9777542.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQENSDTMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTGQG"
     gene            complement(22487..23824)
                     /gene="tssK"
                     /locus_tag="ACHOS5_002751"
     CDS             complement(22487..23824)
                     /gene="tssK"
                     /locus_tag="ACHOS5_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9777543.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSDRSLVDIVLA
                     LPLLNANGGNLDDGRESGRPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSI
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQKGVMWEGAL
                     HDARLREGADFWLSVRSSMPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            complement(23840..25384)
                     /gene="tssC"
                     /locus_tag="ACHOS5_002752"
     CDS             complement(23840..25384)
                     /gene="tssC"
                     /locus_tag="ACHOS5_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9777544.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNNWIRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            complement(25405..25902)
                     /gene="tssB"
                     /locus_tag="ACHOS5_002753"
     CDS             complement(25405..25902)
                     /gene="tssB"
                     /locus_tag="ACHOS5_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9777545.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGSEESISLTFRNMNDFT
                     PEQIARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            26401..26610
                     /locus_tag="ACHOS5_002754"
     CDS             26401..26610
                     /locus_tag="ACHOS5_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9777546.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            complement(26630..26863)
                     /locus_tag="ACHOS5_002755"
     CDS             complement(26630..26863)
                     /locus_tag="ACHOS5_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777547.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(26952..27575)
                     /locus_tag="ACHOS5_002756"
     CDS             complement(26952..27575)
                     /locus_tag="ACHOS5_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9777548.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            complement(27664..28173)
                     /locus_tag="ACHOS5_002757"
     CDS             complement(27664..28173)
                     /locus_tag="ACHOS5_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777549.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT         7090 a         8048 c         6491 g         6606 t
ORIGIN      
        1 cataaatcac cccctgaaag tcatcacatt atccacaaac tgctgttgct tcagatatcg
       61 cagtaacacc ctgccctccg gcataaatgt tgttccgtaa cgaaccagtc ctgcgtcttt
      121 aagttctgca tcaatgacat aatgcaactc accaggctga gtctgctcct gcatggtcac
      181 gtcaacacgc ttaatacgtg gctcatactt cagcagaaca tctgaaagca cacacatcag
      241 ttgatgcgcc gttcccggca ttccctgcag aatgcttgtc atatccggca gaccataatc
      301 cggaagatgt ttaaggcttc cggcccgggt attcagaata cgctgcatgt tatcgagcac
      361 cgaaagaata acctgctctt cctcactgac ctggtttaac tccagcccgc cagcgaaatt
      421 gccatagaga atttcataaa gggaaggacg cggcatcact atttatcctt caccggtaac
      481 agcgtcaggg tgttaccgga tacttcaata agacggggcg tgtccggctc aagctcatcc
      541 cggctcagca ccacccgcca ggtatttttc ttccggtcag gctccagaaa cattgctgcc
      601 acaccggtaa atttcgccgc atcatccaga ggcatatcca cggccactga accacctggc
      661 tgcagccata catctttctg tgcaatgata tcgcctgcca gaatttcact gtcccccgta
      721 aaaagcgcct gataatccgc gctgtcaaaa ttccggttgt ctttaagctg ataaatccgc
      781 accacaaccg acaggggaat accggcagca ctggtgttaa tggcctccct ggcacgaata
      841 tcaaggtgaa gtgtttttat ctgtcggtaa aacagggatt tcgtggcaga taccgtaccg
      901 tctgccactc tttgcgtcag gccacaccct gataacgaca gggaaaacaa cgcaataatc
      961 agaccgtaac cagtcttacc agcgataatc gccattttcc tgagcctcct tacgttgata
     1021 atgttcctga acccgctgat aacgccccag actgatggtc attacgcgtg ccgctgaagt
     1081 cgttatcttt gcctgtgttc tcattacagc cgtgcgcccc agttgcggac taccggctgc
     1141 cggtcggcaa ctgagccgga catccggcaa cagtgaacgc ttaacacaaa gttgcagacg
     1201 tacatccaga cggcttcccg gataaacgtg caacaacgcc agcaaatcag aatacagctg
     1261 cccgcccggt aaccagccct gtacctcgct gcccgtctgt gttgaaagct gtaataacac
     1321 ctgtccgttc acatccgtag catgatcgcc catcaccggg cgactcttca gactgaccgg
     1381 atgatgaaca cgcatggcca gcggtgtttt cagcgggatc cgtcgtcggt catgatgcca
     1441 gacacgggcc tgtgttccgg gagccagcag cgttaccagt gatgtcagcc cttcagctgt
     1501 tcttccgggt aacaacatca ggggcaataa cgccagaaaa cgcgataccg gcgtggcaat
     1561 attctgtgca catccgggga tccccagtct ggccagaccg agcagatact gggatgtttt
     1621 atcctgcccc cccgcctcaa aggtggccgg gtacgaatat ttgcgccaga tacggtaata
     1681 ctgggtgatc agacgatgac tgaatatatc gagaaaatcc gctgcggctt catggccttc
     1741 ccgctgctgt gcaatatcac tgatatagtg agccggcaac ggagacgtga ccccgtaaag
     1801 tcccataaac gtcacatgta cagtgggtgg cagatgggat tcttcaggaa ttacagcatc
     1861 ttttatttca ctcgcaggaa accccattcc cgtgtatgga caaaagcgaa ccgcttcctg
     1921 acgggcctgc cagccactgc caatgggagg ctgatctggc tggctttgtt ccagtaactg
     1981 acagaagcga taaaaattaa tatacggaag ctgtttccgg taacgggtaa ttaaccgggc
     2041 aggcgcggat tgtgattttc tttccaccgg atgcattttc cttcaggctg aacaatcagt
     2101 gtgagctgat tgaactgatt catatcggcg tacagggcca ggaaacggtt gagcatttcg
     2161 ccaaacaggt gaatatctcc ctctccggca aacccgttgc catcaagagt tacctccaca
     2221 tcaagccccc ggagcagaaa acccttttca aaacgctgaa tacgatgatg ctgtacagcc
     2281 agaatggcat caagccgacg gtgattaagt tcatcgtccc gccagttata aagcgccagc
     2341 gtgccacgca gcacttccgc actgctcagc atgttaagaa atcctgtccc cagatgactc
     2401 atcacccgcc agtgaaaacg atcttctgtc ggcggataaa ccggtagcgt gggtttacaa
     2461 agattacgta cactgaccgg ggtctgcagt acctgttcac accgatcaag cagcgtactc
     2521 tgcaacgcac gacggggaag ctgaccattg gtacctgtaa tacgtaatga aagcgtttcc
     2581 cgctccggca tccgatccac ctcccaccgc tgaccgccaa ggataagcca tgtgtcatac
     2641 atccccgtga caccccgctt cacccgggta tgataataac gttcgggtgc atcatggcgc
     2701 agcattcccc cacggtgacg gaagctggta aaaggcacgt actctgcact gccggttcgc
     2761 ccggagcccg tcaccgcatc cactgaatat atctccgtat acccatcctg cagacgcttc
     2821 gggcgcagca ggtattcgct ctccagccca ttgatgatga gtggatcggc ttcaagcgta
     2881 aaaaggttaa tcaccggcac acaatgcaga cacagggcat cgtctgtcac aggtaaatct
     2941 gccggccagg gctgactcag aaccacctca agatcaaatc cggaaatacc tgccggcaga
     3001 gaaacattct ccagtccgtt cagatgaaca aacataaact tttcccgaaa ggtgaaatat
     3061 tccagcaata actgataccc actgaaagca ctgtcacctt tcggccacag ccgatcttct
     3121 tctgcaaaac cgccgggtga aaaccatccg tcaatgcgga tccgctcatc attccccggc
     3181 agcctcagat acagcgcagc cagacgcttt gtcatcatca gatgaagggt gctgctgacc
     3241 ggggcttctg ctgccagata cagactcaga cgatgcaaat cgacctgaga ccagtcagcc
     3301 agttcactgc aggcaaaacc tattttcagc actgagcgcc cgtccggctc cgtcgtcata
     3361 acgacattct ccactgccag tggattcagt ggtatggccc gggtggtacg gtaacgacag
     3421 actgtatttc cggggccaac agggcgggaa gtcacttcca gtccggctgg tacggtttca
     3481 gctattttca tgacggataa ccggggggtc agtgccacca ccgacaggga cgggatagta
     3541 cgcaggtaat gaggccagag catactgacc agactttcag taagctccgg caaatcatcg
     3601 tcgattttct ggcgcagtcg tcccatggaa aaggcaaaac cttcaaacag gcgttccacg
     3661 cagggatcag gcgtacccgc tttatcgaga tccagcatcg ctgcccgatc gggatgagcc
     3721 tgtgcaaacg cctttccggc ttcacgcaga taacgcattt cagcgtcaaa ataacgcagg
     3781 gttaaatcgt ccattatctt ctcagaataa agctaactgt tccggagact cgcgctctga
     3841 cttcagccat gcctcaaaca atcgcccgaa ctgaacggca agccggattt cattaatcag
     3901 attacatgtg aatataactg tcagaaatat catgataaaa taaacgaggg aaaataacat
     3961 aaataaaact gtattacccc ccgacagaaa tggcatggtt gtaaatatcc ctcccgtaaa
     4021 ctgaacacat aaagaaaaca ccgcaaacac tgctatcatc cggtaacctt tatgcacacg
     4081 acgagtatta acagtaaatt tgccatctga aaatataccg acaaaagaat atttaagaaa
     4141 acaatgcagt ctgttatcca ctgaactgac atttctgtcg gatataaaac aaatcagctt
     4201 attacaacac catatcggaa gatatatccc catctgttca taacagtatt ttattcttac
     4261 cagtgtattt ttcgttgttg gctcttttgt cagtttgtca atacgggcaa gaatggactc
     4321 cttttcagat acaggaattt tctcaaatct gtatttcctg atattcggcg catagccata
     4381 atgctgcaca aaccatttga caagaggaat aataatgccc gacagaagcg gtaacaccag
     4441 atttttaact atatcctgaa atgttttgtc ctgaagataa tgcatgacag acatctcatg
     4501 agctccccgg attgcacaac acagcagctc tggcagcatc aatggcgatg agcccggcaa
     4561 gaagatgttc catatcaggc tgcagacgca ccctgtcact ttcagttttt gcggacttca
     4621 tacgtaacag tttcaggcgg cgggctttta cctcaaatac cagttcaggt tcccattgtg
     4681 acagggtcag ccgggttgca cgttcatcca gctccgcaag cagatgcagg gcaaggtcat
     4741 tttttccagt ctgttcggca acacgggcca tcaacaggcg cagtagccag ttgctgcggt
     4801 cagactgaat accgggacgt gcctgtaacc agcttaaagc ggcttctgtt ccttcattat
     4861 ccgcaatctg cagggcttcc ggctcaagag ataaaatgtc gttatctccg ggggctgccg
     4921 ttatcgttac agacggttct tccgcccagc gttcaccaca ctccatcacc tgctgctgta
     4981 tccagcttag cgtcacttca tcggcaaacg gcatgccgtc gttaaaagcc agtgtctcaa
     5041 gccccggcag gcgcagaagc aatcctttca ggtcatactg aataatggca gcataatttt
     5101 cttttccggt ctgcaacagc gcctgatgaa tataccactg caggtccagc cacagatgac
     5161 tggctccccg ggcaaacata tcgtcacact gttcaagcag tgacaaccag ttctgctgca
     5221 gatacaaccg tttcagtgac gcacgccgat ccggccccgg tggggcaata cgggtccgtc
     5281 catcagcaga taacggcggt aactgatgca gcgtgtcatg ccggacgctt ttcatcagtc
     5341 gatgagccgc cagccagccc tcaggctggt cacgcagata ttttgccaga acacgggcct
     5401 gtgacagcag ttcctgtccg gaggtgatgg cagataatgc catgacgctg ccggatgtta
     5461 ccgtcggtgc cggcgcggca gcctcctgag gcaccactgc atcaacacca ccggccttca
     5521 tcaggcgatt ttcaagtgcc tgatataacc cgttcagcac cggggcggaa gcctcatccg
     5581 taaaggtctg ttctgccagc cacagcgccc cggcaatgcg cagggtatcc gccttcacca
     5641 cctccggata cagagacaga ctgtccagta tgcgggcgct gcataaccac tccagcgccg
     5701 ccttcctggc ccgtgaccgt tgtggatgca ggtgctctcc aaaacgctgc aacaaaccgg
     5761 tcagcagctc cagaccgtcg gccagacctt tttcaccatc ctgccgcaga cgggcccaga
     5821 tataccaggt cgccacccgg atatctttgg ttctggtggt cagtaatttt tctgcaagct
     5881 ggctgacaat tccggcatcg gctccggaga gtttattgac ctcttcacgc atctgctgaa
     5941 aatcatcatc gtaccccgca tcatcacctg cgggattatc tggcgtcacg ggagcaagcc
     6001 atttatccca cagcgcaata cgcacctgag ccggttcagc cacatcccgg ggtgtcgtct
     6061 ggcatgcact gaggagcgaa tgaatggaag ccatcagtca gtctcctcca cggaatcccc
     6121 ggcatcttcg tcattgcctg taaacgctga cgtaccggag agttcgaaaa ccatttccgg
     6181 cagaacaaaa ttgcggagtt tcagcaatac aagcggccct tcccccgctt cagttcgcag
     6241 tgtgtaattc agcatacgac cgtcctgcgc ctgccagctc aggctccagc cactggcaac
     6301 gcccggtgcg gctttccgtc gggccatctc aagcagacgt atcagccccc agcttcccgg
     6361 caggtctgca tactgacggg taccggcctg agtgcttacc cagcttaaac tggcgccagg
     6421 tgcttccgta tcagccggcc aggtaaatcg cttccatacc ggcatctgat taacataaat
     6481 cagtttctga ttatcggtta tcagcgtcgt ctgcatcaca ccggcagcag ttcccgggcg
     6541 cagctcaaaa tgcagccccg cgttacctgt ggtgaacgcc acatctgcta tttcactcag
     6601 ggtattgatg gctttcagaa atgcagggtt gaatgtcagc ccccgggtat taatggtatc
     6661 cggtacccag cgattgcctt ccctgcgcaa cacaccactc aggttattct gcagaaaacg
     6721 ggcaatacgt cccgtgtccg tattcaggta ttttgccagc aggggaagtg aagcatcgct
     6781 gctgacattt ttaaacggat aacgcccgga aaatgcatta ttccagccat ccaccaccgc
     6841 agtacgccac cgggtattca gactttctgc cgcgggtgta agaacctgct gccacgcctg
     6901 ctccatcggg cgaacaaaga ccgtctgtcc gaagccatac cactcctgtc cgagtccggc
     6961 agcggccagg ctgccgtaat cccgggtatc agttaaatca accgatttcc cctgcaatac
     7021 tgtctgtgcc agcatctgca tcatggcctg cgggtcagaa gagccggcaa tctgttgcag
     7081 acgcagacga acctgtgtaa tccgggtcag atacgtctgc agattcagca gatccgcgct
     7141 gttgttctga ttatccatca gtgcaagcac cggcccgaaa gtcgtcgcca gaggaccgtg
     7201 aagccggctt tccggtactg tcactggctg tttttcctgt gaaagcaggt ttctggcaga
     7261 tttaaccagt gaatcagtaa ccgcttcccg gggctgacca gtacgcccct gaaccgccag
     7321 cgtattcatc agtgcaacca ggggggactg gcgcacatca gccatcagcg tcagttgctc
     7381 tgtcacatca gacagcgtct gagctttacg caagtgcaga ctgttcagaa aattcagcca
     7441 ggcattacca aaatcagcaa aatagcgcgt tgtcaggcgc tgtctgagtg cttccggcga
     7501 taccggtgac ggtgccttct gcctgccatc cgtcagtacc cagtccattt cttcacgacg
     7561 ctcattaatc acggtgtcta tcgccggcaa tacggcctct tcccaggcct ggcgggtgaa
     7621 cataccggga accacttcat ccgtaaaaaa gaggcgggag acatcagtat caccggtcat
     7681 atccgtcaga cgcatatccg caaactgatg tgccacccgt gccagcattt tctgatacag
     7741 catggcatcg ctgttctgtg tccccagatg gcgaagcagc agagttctgc tctggctgac
     7801 cagctcttca tcagccgtca gcttccactg aggatgagta ataatcccgg aggcataata
     7861 cgccagcaac gtcgggccat tagcctgcca gaaaacggca ctcacaccgt cacgctgtgg
     7921 ccattcccgc atgagcgtac gactgaacca ggcgggctcc atatgttgcg gttgcgtcag
     7981 catcagatac agtctgagct ggtcatatgc ggcttttgcc attttccccc gctgcgggct
     8041 gtccggagga agacgaataa atgtcctgag ctgctgctca agatgagcag cagcggcatc
     8101 acgcagtaaa agatgtgcat tttgtgcata aaggggcatt acgaccgcga gcaggtctgc
     8161 attctgattc ataccggccc gcaggtacca tggcgccccg tgctcctccc ggtactgaag
     8221 gcgggccagg gatttctgca gattcagcaa cgcctgcaga cgttctgcag gagacagttg
     8281 ctggttctga gccggacgaa tctgttcctg cacttcagca accagactcc ggtttgccag
     8341 aaaggaaacc accattcctg ttccctgagc catcatcagt atggccgcca taacggccag
     8401 acttctgcgc cagttatgac ctgtgcggga aggctgtagc cggacaggta attgctgcac
     8461 tgtctccggc aacgcctccc agcggttatc cattcgccag cgatgtctga cacttcgttc
     8521 accgccgaca gttgccggac tgaaaacaat acccgccagc aacaactgac ggtatggacg
     8581 gagtaatacg gataatggct ccaccacagc atcaatattt cgtctgaatc gctctgccag
     8641 cgacaacaaa aaataatacc gaggggcaca gcatatctgc tgtatcccct gagcaacaag
     8701 cccggggagc atggcctgta gttgtgcttt cagtttgtca gaactacatt ctgccggcag
     8761 aaggcatccc acgggctgag taatacgccc tcgctcatcc ggactctcct gcagtgacca
     8821 gacatacagc ggcagacgcc agccaaggcg ctcacaacat gaactgaccg cacgggaaac
     8881 cctgtccagg gcatcttctg tcagtggtgc ggataatgtt tcactcagtc cggaagttac
     8941 ccagacaatg ccatcaacag gccggtaacg cagacggcgt aacgcagcca gccagtctgc
     9001 gttttccggc tgcgaggggt ctcctcccca gagaagcaat gtcccctgct cttcctgcca
     9061 gaactgttct gtcaggcccg gggtcagcaa ctcaacctca gaggcagtac ctgttatcaa
     9121 cagaatgcgt attttacgtc cccagaatct gccatactgg cgccgcagag tatgacgaat
     9181 atcctgaaca gtaacatagc gcggtggcgt ctgagccagt cgctcttcat cgtcaggtaa
     9241 cctttgctcc ttccgggccc gacgtcgata tgtcagttcc tgaacatatt ttttcatcac
     9301 aggcaggtaa cagacaaaga gcaaccccag actcagcaat atcagcagta gtatttgatc
     9361 ccggcgtgtt tctgccccca gaaatgctcc atatcgggtt atcagccacc agagcgcagc
     9421 cacaccaata aatacaatcc ccggtcgtcc aaagcgacca gacagacagg ccagtttatt
     9481 cattctggtt tcctgatgta acagtactga taaaatgttc ttctccggat gagaataaag
     9541 tcaggagaga atcattacca agacagacca gatcggctgc cagagcagta agcagccacg
     9601 gagcagcagg tcccgggata ccgcaccatt tttccagtaa catacgtccg gacggttccc
     9661 atccggcacc atactggttt ccaatagtca tcagcggtgc agcaatcttt tcccagtgat
     9721 aacagtcccc cagtacacga gcggtacgac aggcagctgt ctgggtttca aggaaaagtg
     9781 tgaattcatc attaaacttg ttgatatcca gcgacatcgg gcgtagcaga cgggctggat
     9841 gcggcaggtt atatttttgc gcaacatcat cgctggtcaa caaaagtgca gccagtccgt
     9901 cagagtatgc attcccccca tttagctgta ttaccagcat caaatccaca gtcagcactg
     9961 gttgtttcag acgctcatca acccacccca tggaaaagtc gggagtaacc tcgatattgt
    10021 caggaacgac gcgttgggga aataacgctt cccatgctga aacaaaagca gatctgattt
    10081 cagaatcagg ctgatcggtt atcagcgtca catttacagg gagagcggca ggtaactgac
    10141 aaaatttatc cgttatctcc cttagcaaga cacgtaaaga ggcttcaacc ttatgcccac
    10201 tgtcagaaag ataatctatt ttctttacaa ggccgtactg cagaggcaat tcatcacaaa
    10261 gagtagctac cgttatttta tcaggcaaat aaacacaact ggcagtaata acaagatacc
    10321 tttcggccca ggattcccac tgtttctgag catactcccc ctctttttta agaaacaaat
    10381 gttcatccat ctggttgctc cactgccagt gtcgcaggca aaacaggaaa aaccagcacc
    10441 ccgaaggagt caatgcaagc caccagatac tgaaatcatt taatactttc acatatcctg
    10501 atgcccggat aataaacaac agagtaccaa caacagccat aaatgcccct gtcataaacc
    10561 acaacatcat tgatggttta tgaggtaatt ctgttgataa agattttgtt ctgctccatc
    10621 ccatggttta tcctattgca cattccggtg cactggatat aaccttacac ccacacagac
    10681 atttacatcc atcaatcaca acagctcttc catcagatat ccattcagga gagccctcca
    10741 caataggatt ggtaccatgt ccggaaacag ggcaactaac catatctcca acaagagcta
    10801 cttttttacc atcgacaatc atggttgaag atgccgaaat aactttacca ccatgtgtgg
    10861 tattatcacc aagcaataca aaacctttag acataaaact ctcaaaaata aattttcact
    10921 ttgagtcacc agttaccgta aggataagtt ttatctttga taaattcctt aatatcttca
    10981 tcatatccat atgtgaaatc acttttagta acccccttac acatattcat tttatcaccc
    11041 gatactgtag taattttagc atccaactga ggtaccaccg gattaccgcg atctcccagc
    11101 cagactctta ccttcccttc aggtgccaga ccaataatca tcgttgaacg ataataggtt
    11161 tcgttccagc ggttgtctgt gtaaggcgta accatctgct cccaggttcc ttttttaaaa
    11221 aaaatcagag tttcataact ctttttatca attatcgaat cccagcaaaa acgcaacatc
    11281 agaggtaatg ctttcccctt gttataatac gactcagtcg cgcccagatg ctcattccag
    11341 cgccctacac tgacctgact cggtcttgtg gtatcaagat actgataagt cgtcttataa
    11401 ccttccccgt ccaccatcaa tgcctgagtg gccagcgcag gcagagcctt tggataaata
    11461 aagagaaatt tccattcatc atagggtaat tttttataac cccattcagt cttatccgaa
    11521 tcagcatact gtgagctaac agaattacac ccagttaaca atgccaataa tggtgcgata
    11581 aaaatgattt tatctctggt catgtattgt catcctgtag cgttaaacac cagacatcat
    11641 aactttagta ttttttactg cgttatcatt caatcgctgc tgatcatcca tgtaccatac
    11701 agaacgaatc cagcgatcat ttggtttatt cggaaaaacg aacgccttat tacctttagg
    11761 accaaagcga tggataaaaa tctctcccgt tgtaggatcc acccataaat taccatcaga
    11821 ctcaatattc cagttggcag aacaatgggt gtattttccg ataatataca actcttcctc
    11881 agtaaaagcc tgaggctctt gccccaaacg taccgctttt ccctgagcaa tggctttatc
    11941 aacaaaagga attaattcag gagataaatt catatctgga tttttggggt caattggagc
    12001 aaacaatacc cctgcttcct gagcagcatc cagcatcaca cgcaggcaaa cattagccca
    12061 gtcattaggt actgccatac gctcaaaaaa tactgcggct cccacctgct tttgtatcac
    12121 gccagcccga cgttgattgc tgttgttcac acctacggaa cgaattttgg tggttagttt
    12181 tccatgaggc aaaatatgtt ttaaggctgg cagagtcatc aacatttttc tggcttgctc
    12241 tgcattacgg tatactcgcg tatctgtttc tctggtatcg tcactgacaa tttcaaatgc
    12301 cgggcaactc agaaacagac tttcattttc ctgcagaggt gaacctaccg gattgtaacc
    12361 acctccaata tctgaatgcg cccctggtaa agccagttcg ggccacatac ctttaatact
    12421 gttcaggctg aaattatatc ggtattcatt cattgcagta atctggaaaa ctttcttcgc
    12481 gatggaagga cgcaattcca gctttactcc cgggttgcta cgtccattaa tatcgaagaa
    12541 attactgatt ccaccaatag ctgcgacagt atcaaaaagc cccagaaaac ggacttcacc
    12601 cgaaggttta ccatcataaa aatcgcctct gagcccttta gcaatagctc tggctatagc
    12661 aggatcctgt tccataaccc gattagcaaa gtgccttgcc gccgctgctc cccggctaaa
    12721 accaaaaaca tcaaactgga tttttgcgat acaaaaatca ggatttaaat tgaaccccat
    12781 aaattctgat aacgcttggg tgattcgttc cattgcctcg tcggttttcg tcaccacacc
    12841 ttcaaatata tccagaatac tggtgcctaa tcccatgcca atgactgaat ctgcttcacc
    12901 agccgcagtc cctatcccgg aaatgtaagt ctgaatttgt gcttcaacct gactttccaa
    12961 caaagtctga tctgctacgt aaagtgaatt tagccagtgg atattggaat aatatccacg
    13021 ataactgcca ttcgagatac tgttcttgtt aatttcttca agaaagtcct cacagctttg
    13081 tgcatctttt tgatttaccc cctgtctggc tgcactacac tcattaaatt tcatcagacg
    13141 agatgctgta ttctcccgat tgtttccggt accatcgaaa aatataccaa tggttaaagt
    13201 cacaccccgc gccggttttt tctttgcaac aacaactttc cagtcattat gaatactggt
    13261 tttatgggtg gcatctgtta catcatatcc ctgatatttt gtcattctgt ttctccatga
    13321 atttttacct tcccaaactc aacttttact actgacggaa tggaggtgtt gactggcgct
    13381 gttttaccat tttcatcggt gatcccctgt agaatatcac cttcagccgt tgttaatcga
    13441 taaggcgtcg aaggttgcgg cactcccgct tcatcggtaa gaataaaacc gccaccaaac
    13501 cctcgcggca tttcaacaga cgcaatatca agacttgttg gtcccatctt ctgcatattc
    13561 gtgcttttta acaggatctg tcccgggcat cccagctcaa tatttccacc gcttagtttg
    13621 atataagctc caccacatgc cagtatcagt tcctgattag cagaaacagt cactttgccc
    13681 tcagaacttt gaatatcaag attctgttgg gaactcattg acaaatcgtc actttgagcc
    13741 tgaatattta cccgcccttt tgctgctaac agttgcatcc cctggtttct gctcaacagg
    13801 ctaacagcac cttctgctac aactgtaaat gactgcccgg cactaatatc agtgttatct
    13861 cccgacatta tgcccacgct ttcactaccc gaagcaattc gtaacgcccg tgcactaacc
    13921 atgccaatcc catcaggagc atgcaacagc attcccggcg cagtgagatc tgttagcgat
    13981 gaattcaacc gttgctgaca acttatatct ccctgagtag catttgcagc agttgcggcc
    14041 ttcgccatac ttcgggccag agataatgcc agctccagtt ggcggatagc ttcattcata
    14101 tccagcatct ttccttgtgc ctgaggctgc atatctgcac tgataaatat tccttttcct
    14161 ccccggatac ttatccagtc atcagtcctc agctcagcgc cttctcccct gagtttccgg
    14221 gaggcattaa cgttatgacc aagattaagt tgtgtcttac cgccaaactc ggtactgatc
    14281 ttgatatgct cctcaccgcg tttgtcctcc atccgcaact tgttatttgc cggcgtacgg
    14341 ataacattgc gcgtaccgtt cttatcggta acgtgatcaa catgacgtga atcatgcagg
    14401 gcatgcgcga tataggggcg atccggatca ccttcctcaa aggcaatggc cacttccgtt
    14461 ccctgaatca gcggaaaatg aaaaccataa gcatcaccac tgtagggttt ggccagacgt
    14521 accggcatgc tttcctgtcc cggattttta tcatcacggt cagcatcaaa tttcacccgg
    14581 tacatacctg atgcatcctg ccaggcgtag atatcccctt cttttgcact ggtcacacgg
    14641 gccgtcatgg taccggtaac tttcggacgc ggcagtaaag gaggacgcca gcagataact
    14701 tcactgtaag gaacgccctt cagtacaacc tgcagggcat ccttgcgact tgcagaaaaa
    14761 ccgacacagg taagcaacac cggattgcgc agcagcgcag gcaacgtcgg cggaagactg
    14821 tcggtgatag tcagaacctg agcaggtgcc agcgtggcgt ctgtactgtt gccggttatc
    14881 cgcacctgct ccgccagata acgttcatga ccaaggcggg cataaaaatt ggccgtctcc
    14941 ggcaccgggt cgattttatc cccccgttcc agatgacgga aatggtagtg gtacacctca
    15001 ccatagttaa ttccgtcacc atcaccatgc gtcatgtctg ccggggcgga ctgcaacaga
    15061 tattgtgctt cacgatggtt ataagccttt gtggtgacac tggcctctgc cacattgtgc
    15121 gtcacattca gcccccacac tgaatccgct ctgttatcat tcattccgga cggactgttt
    15181 atcgccagcg tcttatcaaa cgtcagcgca gcctggctgt caccaaaatg cactacttcc
    15241 gtctgcgtgt ccggttgcag ggtgaaaaaa taaaaaatcc ccacttcagc cagcaaacgc
    15301 tgaataaatt gcaggtcact ttcctgatac tggttaattt gttcacgctt cggatacgtt
    15361 tttttgagac ggaactcaaa ctcccagcct ttcaggccgt gctcctggag aacctctgtc
    15421 accacctccg gcaccgattt gttaacgaaa aatcggtgag aacgatgctg cctgtccagt
    15481 agtttaataa atggttccag tatcacctgg tattttgcct ggtcggcgga gcccgatata
    15541 cgacgaaaat ctgtaatgac accatgtact actttctggc tcacctgctc cttaagccct
    15601 cccgtaccca ttgtaaatgt ggctgattta cgcagcagca gcccggcatc caggtcgttg
    15661 tcagtactgg taaaattgat gatgtaataa taggtctgac tcatctcctc cctgccgctg
    15721 aaatgctcaa catccagtgg tgacgggcag gagggaataa ttaaatggta acgattcagg
    15781 gaagcagaga tagtttttga aagtgtatct gtcagactca tattgttaac tccatgtctg
    15841 taccggtatt gaactgcaat tatataatga tgaccgttaa gctttatttt cagagagaat
    15901 tactgataat catcccattc atcgtaaata tatgcatatg caaatcgata catcagataa
    15961 ccgacaaaaa ataacgcaac agagagccct ccccacagat atttaaattc agcagatgag
    16021 agaaaaaaga accagactgt aaatacaata cccgacgcac ctaaccccat acctgcgcca
    16081 cggataaatc gggaaagcat tataagtaat gttcttttta tcttcataaa ccaccgaaaa
    16141 aatattcgca tatccggtca aactcagccg aatttcggtc tgcgacaata caggctcgtt
    16201 caaacgggcg cacaatatct tcaacaagaa agtacaaaag atccaaatcc ccctgagctt
    16261 tcagcttatc atgcaacacc ggatttctgt ctttcagaaa acgggatgtg taaatcgcac
    16321 gcgatgtctc tgcgccaaca gataataaca acccggcaaa actccctgca aaaaaagcag
    16381 ccagccctct ggataccaga acctctccca gtttaacccc ggtcaattca gcaaatgcca
    16441 ttctacctgc cagatctcct gaaacgttct tcacaatgga gcctgttttt tccatattga
    16501 tccgggatac aaagtcttca agacaaatac tgataatttt cccaataata tcacggctga
    16561 aaatgccttt ttctaccagt ctggccattc tgaatttgtc attctggttt tcccgcctat
    16621 gcccggtatc tataaaatca tatcccatat aaccgagact gactggcaaa gatatgagtc
    16681 ccttcccgac ggcccccaga aacccatccc cattaatatt tacggcctcc agcatatccc
    16741 gggcaatttc tttagcactg tccatatatg ctttcctgcg gataatcgct attgattcca
    16801 tcaaacgcta tcacaatacc atcctcatca ttccacgtca gagacaggta cgtcggatgc
    16861 tgtctggcag caagatagct gagcaactgc cggctcagta ccggcagcag ttgctggttc
    16921 agcagactct ccacattccg ggcgcccgta tccggcagca ggcaggcgga tgtcagggca
    16981 tcgtacacac gttcatcaac cgatgtgctc agcccataat ggcagtgcag gcgttcgcag
    17041 acctgtgcaa gcttcatctc cacaatggtg cgcatggcag actgcgtcag cggacggtaa
    17101 atcacggtcc ggaaacgggc aagcagtgct ggctggaagt ggtcgcgcag cagtggacgg
    17161 ataagctcat gcagctccga ttccgttgtc tccggctttt cgctaagttg ctgcatcaga
    17221 aggtcactgc ccagattgga ggtcatcaga atgaccgtat tacggaagtc aatttcacgc
    17281 ccctcaccgt cgcgcataaa gccccggtca aacacctggt agaacagatt cagcacatcc
    17341 cggtgggcct tttccacttc atccagcagc accacgctgt aagggcgttt acgcacagcc
    17401 tccgtcagaa taccgccctg accgtatccc acgtacccgg gcggtgaccc cttaagctgg
    17461 gaaaccgtat ggggctcctg gtattccgac agattgatgg taatcagtga tttttcgccg
    17521 ccgtacatca cgtctgccag cgccagtgcc gtttcggttt ttcccacacc actgggaccg
    17581 accagcagga acaccccctg ggggccgtta ccggatgtca gacccgtttt cgccgcacgc
    17641 agacgctgcg caatggattc cagtgccacg tcctgtccga ccacccgtct gccgatatcc
    17701 ttttccagat gcagcagttc cgtctgttcg tccttcatca gggaagataa cggcaccccg
    17761 gtccggtccg cgaccacacc ggcaacggtg cgtacgtcaa catccacact gagcaggggc
    17821 tgattctgct gaatgtcgtg cagtgcctgc tgcaggtgat gtgtctcact ctggcgggaa
    17881 atatcgctgc ggctttcaag taactcaccg gtgagttcgc actcccgggc gtactgctgc
    17941 tccaggtcat cacgtgctgt catcagctcg ttctcttcct gctcaatggc agacagacgt
    18001 tcgccgtgat tctgatgacc aacggcaata tcttccagca gtgcctgttt ttccataccg
    18061 agggaagcga gctgtgaacg gatacgggtc agctgttccg gcaccgtgtc gaggctcata
    18121 cgcacacggg cagcagcagt atcgagcagg tcaacagcct tgtccggtaa ctggcgttct
    18181 gtcagataac ggcgcgacag cgtgacggca gcacgtaccg cctcatcggt gatatgcacg
    18241 ttatgatgtt ccgcataacg ggatttcagg gagcgcagca tcagacaggc cgtctcatca
    18301 tccggctcgt ccactctgac catctgaaaa cgccgctcca gagccgcgtc acgttccagg
    18361 tattgtttgt actcagacca ggtggtggcg gcaatggtac gcagttcacc ccgcgccagc
    18421 gcaggcttca gcaggttggc cgcatccgcg ccgccggcct gattaccggc cccgataagg
    18481 gtatgggctt catctataaa cagcagtaca ggagccggtg actgctgcac ggcatcaatc
    18541 acgtttttca ggcgctgttc aaattcaccc ttcacgccgg caccggcctg cagcaggccg
    18601 aggtcaaggg tgcgcagggt gacgggtctg agtgattccg gtacgttacc ttccgcaata
    18661 cgcagggcga gcccttccac cagagccgtt ttccccacac cagggtcccc caccagaatc
    18721 gggttgttct tgcggcggcg ggagagaata tccaccatct gacggatttc cgtatcgcgt
    18781 cccgataccg ggtcaatttt cccctctctc gcgctggccg tgatgtcccg ggtgaattta
    18841 tccagagcac tctgtaacac aggcggcagg tcacctgacg gtatacccgc cgtatccgca
    18901 cccacgggac gcccgataaa ggtcacattg tctccctgag gcagcacagg ctctgcatcc
    18961 tgtaatctct ccggacactc atcagactgc gcatccagca gcggggacag acgctgcaac
    19021 tggctatgac tcagtgtcag cagcggccac agcccgtcac agcgaaccag gtgcggattt
    19081 tccgtcaggg cacccagcag atgcacgctg cggatatgtt cttctccctg taatgaggcc
    19141 accatccagg cctgtttcag cagggcattc agggacggcg caagctgcgg gcggctgcgt
    19201 acgctacggg gctggttatc cagccagccg agcagcgact gccatatcgc gtccatatcc
    19261 cagtcataac gccggcccag cacggtaagg tctccttccc cctgctccag cagtttcagt
    19321 aaccagtgct ccggggtgat ttctgcatgg gcccgggtct ggcaaaggga ggctgcgcct
    19381 tccagtgcac gggcacagta atgattaaga cgacgtaaca gggctgccgg attttccatg
    19441 ttattcactc actgtcagga gaccgtcagg cacgctaccg cccttcgccg taacccgggc
    19501 gcatcaggtc tgtgttcagg actgagaatt ctttcttcag cacccgccaa cagatactga
    19561 agaaaaaaga aaaatgacgg acaggatacc ctgcccgtcc gggagaatta cgcagtggta
    19621 cgttcattcc acgcgtcaga atgaatgatg ttaccgtcct tataagtcca ggtgattttc
    19681 tcgtagcgca gctccacctg ctcaaggtgg ttatgcttct cgtaggcggg attcttgatg
    19741 tcgtgcatga ccgggttaac cttcaccacc ttcacgttct caagcttcgt attgaaatac
    19801 tccacttcct gaccggcatc attgattctg taccacttaa actcagcaga cttcagggtc
    19861 tgccccgtgg tcaccgcctt gtaaaggtag gggctggaag aatcaatttc cttggtaaac
    19921 aggaacggcg tgtgaatacg ggtaccggtc agcttaccgg tattgttatc cgtcggaatg
    19981 taaaggcagt gctcctgagc caccacttca atactgccct cacggtcctt cacatccacg
    20041 gagcctttaa tatccgctcc gccatcatcc tgcagccaca gataaacagg aattgccata
    20101 aagttactcc ctctcgttgt cttacgcgcc ctgttacggc gcccgggtga tgtccggcag
    20161 acgacaggca tcgacctgcg gtaccagact gatttcaaca cggcggttac gcgctcgccc
    20221 ttccgccgtc tcattcggag ccacgggacg actctcacca tatccctgta cggcaaaaca
    20281 gctttgaggg atatcgcccg tatcccgcat ccagtcgcga acggcctctg cgcgcttcag
    20341 tgacagggcc tggttgcgct gcgggtcacc cgtattgtcc gtgtgaccac tgaccacaat
    20401 cagccagccc gcttttgcct taatatccac cagggcattc accagccatt ttgtggcgcc
    20461 cggtttcagc gtccatttac cggcatcaaa cagcgccagg ctgttaaggc gaaccgtttt
    20521 cggcgtctga gtaactttct gtatcaccgg acggggaggc agtggtgcaa cccactcacc
    20581 agtcgctcgc tcaaccggcg gtatcagcca cattccctga tacaatccca gtccgtaacg
    20641 ctgcggcaca ccatgttgct gccagtcgtt cagtaaccgg ctgtcagcct gaagacgttg
    20701 cagtgcagtt gttttcaatt cagatgaata tccgcgcatc cggtgccagg ccgtcaggtg
    20761 ttcccccaca ctgcgaacca gtcgctggtt attcataaaa gacgccagca tagccagcag
    20821 aagaaaaaca ccgacaatca accccgtctg gcgacaaagt ctgtgccagt gactgagtcc
    20881 tcgatgacgg gggataccgg acagcagaat atccggcaac ggcagtacct gtgatgacac
    20941 gccggcagac ggtggcagtg cagtgaggga cgtcagttgt tgttgccaga tattaccggg
    21001 tcgggcagca accgacgtca gacagaatcc gacggcaacc ggccggaaat aaaagtgctc
    21061 ctgccggcaa atatctgtca gggcatcagt aacccggttg taccaggaca acatggcatt
    21121 cagccataac agttcactga cgcaccggag cctggacgct gcatcacacg tctgcagcca
    21181 ttcctctgcc ggaatggctc cggcttctgt caggcgtata tgcattcccg acaggtccgg
    21241 tgtcagtgta aaccagtgtg cagagtgctt atttaaggat gtgttttgtg gtgacggccc
    21301 tgaaaccatc aatgtcacca gcaccggcgg catggtattc agccactgcc ggcactgcat
    21361 gactgcacgc tgccagttcc gcaaccacga caggaaagct tcatctgagg tatgctgctc
    21421 cggtaaaagt cccagcatga ccacgacctg gccacacagg gccggacggc tgtacgacaa
    21481 ccgctctgcc agtattctca tctgctccgg cgtatccaca cgaagatacc atcccgatgc
    21541 tgtttcccgg tatgcctgac cgggttcgaa ccacgcatct gagtttccgg caacaagcag
    21601 cacatgcccc tgaaaatact caggcggcag accgtcatcg ccggcattct ccgggatatc
    21661 acgacgggaa gacagacaac gccactgata ccacagcaca agccccgcca ccacgactga
    21721 aaccagggtc agtattatct ggtttactgc agacagtggc aggaatcccg aaatcagcca
    21781 cagtgtcagg aacagactca gtattgtcag ccagagacgg gaacgtacgt gcatggttat
    21841 ccctgcccgg taagccgtgc cacctggtct gcgagcacag aagaaagcac acaccagaga
    21901 ccagccagcg tcataatacc cgccacccat gacaaccagt acatcgttct gccagcacgg
    21961 taccccggtg cacgcaccac caccggcgca tcctgtgcga aagtaaatgc aggaacacgc
    22021 gctgtcagtg cgcgtattac atcttcgcga cgctcatcat cctgcgaccg gtactgaccg
    22081 gtaaaaccga gctgaagcgt acggcagagg catgtcagta ccgccacatc cggcgctggt
    22141 aatttcagtt gctcacgaat acgctcccag agctcttcac cggcattgag cgtaccaaaa
    22201 aaatgagcct gcagcgggtc ctgctgccag gtgtgccagc catcgtctgt tttttcgcgg
    22261 ttcagcacac tctcgtccag cagggcacag aaggcataca acatggtgtc actgttttcc
    22321 tgactgaatc ctgcttcggc cagctcttca cgcgcctgtt tcaccagctg acaggcacgg
    22381 cgatacaggg ccttaccatc ctcaaccggt tgtccgctgc gcagctggca gaccatcagc
    22441 cagccgggat aaaaaatctg ttcagcccgg gaaataactg gtttattcat gtccgcagca
    22501 ccgcaaaaag ttccagtttc acatctccca gcgatgccgg ggtgtagaaa gtacaacgcc
    22561 ccgcgtccag catcgcccgg gccgcatccg tacttaaatc cagcgcgaaa tactggtttt
    22621 ccagccggag cggaatggcc gccggtacat gcgtaaccgg acggataatg accccgctca
    22681 gcgccacatt caccacctca gacacatcat cggggctccc ggctttgcat agctgcggaa
    22741 acttcgtctg cagttcatgc ccgggcatgg aagagcgcac tgaaagccag aaatccgctc
    22801 cttcgcgcag gcgcgcatcg tgtaatgccc cctcccacat cacacctttt tgtttcagtt
    22861 caatgaagac cacacgggaa ggcaggcttg cctccagcag ctggttcagc aaagaaagca
    22921 aaggtgggaa aacattttca ggtgtttcat gacggtatgc cggaaccgca tccacattat
    22981 gctcaagcga gaatgtgagc agggagcctg ccagccgggc aagctccctg tacaacagtt
    23041 ccgggtggcg gtaaggcatg tcaagcaact ctttcagaac aggctccgca ctgttcaggg
    23101 cattcagcaa ccagaacaac gacacgtccg ccactgcaaa atccgcaaga cgggcattgt
    23161 tctcccgacg tatggacatc agtcgctggc gacgcgcctg caggtgatga agcaactccg
    23221 caagctcagt catcagcgaa ggactggcag acagcgtcag cagaggggga ataaaacgcg
    23281 ggtccctgca ccactgtccc tgtgcatcac gaacaagacg ggtaacggga caggtcagcc
    23341 aggcagcatt ttcctgatga gccatgcgca gagtcaggtt atgccgcaat accgccactt
    23401 cgctttgctc atgcccggcc agctcctgca cattcaccct ttctgatttc cagcgacgcg
    23461 gacgcccgct ctcacggcca tcatcaaggt tgccaccgtt agcattcagt aacggtagcg
    23521 ccagcacaat atccaccagg gaacgatcag aaaccgtcga taaatcacag accggaggca
    23581 gattatccgc acgttccgta tcaataagcg ttccgtccgg gaaacgaaca atcagacggg
    23641 tggcattcaa ccgtgaaagc ggtaacagag agtcatcaaa ctctgcagcc accacgcccc
    23701 agggatgcgc cagccccatt cgggcaacgc tgtctgccag atgaacatcc caggcagcct
    23761 gctgctgaaa ctgctggggc atcagaaaag ccccgtcttc ccacagtgga cgataaatct
    23821 tcatttcccg acgcctgcct tacgcttttg cctttggcat ctgcgaaacc agtgacagac
    23881 tgacgtccat accttccacc tggaaatgtg gtacagcata gagtttcacg cggaaaaatc
    23941 ccgggttgtc ttcgatgtct tccaccacca cttttccgtc acgcagcgga tgcgaggcct
    24001 gcaactcatc acccggatcg gtcatctccg tcaccagacc ccggatccag ttattcagct
    24061 ccagctcaag cagacggcga tctttggtgg taccgatatt ttcccgctga atcatcttca
    24121 ggtaatgcgc aatgcgggac agcaggaaga tatagggaag acgggcatta atacggctgt
    24181 tcgccgttgc atccggcgta tcgtacagtg ccggtttctg ggtggagtta gcggagaaga
    24241 agcaggcata atcccggttc ttgtagtaag agagcggaat aaagcccaga ttagcgaatt
    24301 caaactcccg agtttccgga atcatcacct ctgacggaat tttgacctga ttaccggttc
    24361 ccaaatcata cagatggatc ggcaaatcct gcaccgcacc accggcctga ggaccgcgga
    24421 tctgaacaca ccagccgttg gtgacaaaac tgcgcaccat attggccgca aaagcaaaag
    24481 acgcatttgt ccagagatac ttatgatgat ccggaccttt cacctcttca acatagttaa
    24541 agctgcggac aggtacggta tcaggaccat acggcaggcg tcccagaaca cgtggcatca
    24601 ccagaccaat atagcgggca tcatccgttt cacggaaagc gttccacttg atgtactcgg
    24661 cacgctcaaa gtgattgcca atatccttaa tggcggccac ctcttccatc gtatccttcc
    24721 ggaagaaagc cgggccaact gaaccaataa atggcatatg cgcggacgca gccacccggg
    24781 aaatattttt cagcagcgcc acatcctgcg cagacgcatc aaactcccag gcagaaatca
    24841 gggctgcaat gggctcacca cccggcgtat cgtactccgc cacataagtc tgcagataaa
    24901 gaccactctg aataatttcc ggcgcatcct caaaatcctg gcgcagatcg tctttggaca
    24961 gatccagcag ctcaattttc acattacggc ggaagtccgt tttatccacc agagacttca
    25021 cgccccgcca cagggattcc actttctgaa attccggatg atgaagcacg gcatccagct
    25081 gacggctgat ctgataatca agctctgcaa tatgatggtc aatcagcgcc ttatcgagtt
    25141 tctccaccgg tgctcccgct ttcgccaggc attccataaa cacctgcatc cctgcggtca
    25201 gacgttcatc tgctgatgcc tctgacatgg cctgcggatc ctgccagata tccagcgcac
    25261 tcagggctga taccggattc agattaattt tttcaaacag ggaagcatat acgctgtcgc
    25321 cggaatgcgt ttcagaaacc gcatttcccg ccacattatt ttctttttgt acagacatca
    25381 gcatgctctc tcttaatccg ttaatcacac ctgttccggc accagtgcac tcagttcgtc
    25441 acgtaaggcc tgactcagtt cgggatcccg cagaattttt tccagttctt tacggaaagt
    25501 ggcgttatcc agcagattcg ccttcagatc ccgcagaagg ctgcgcatgg ccagcatggc
    25561 tttaagacgg ggaatttgtc gggcgatctg ctctggcgtg aagtcgttca tattgcggaa
    25621 agtcaggctg atgctttctt cagagccatc accggcaagc gtgttctcca cattcaggcg
    25681 tactgacggc gagatatcag ccagaacagc attaaagtta tttttattaa catccatttt
    25741 tatgcgctct gacagcgggc gctcttcctg tccgttactg aaatcaccgg caaccagcag
    25801 tttgagcgga agctccactt tcttctgccc accacctgta tgtaggtcta atttcagatt
    25861 cacccttgcc ttcgggatct cattctgaaa cgaactgctc atgacgttct cctgtgaaac
    25921 ctgcatccca gggagcccat cgccacagaa ggcctcccct tgtgcactga tttaatttca
    25981 cccattatag gacgaagatc tgcaaattct caatatgaca gacatatcga tcgttttaat
    26041 gccaataacc agatctttat tctgatacat atcgcaaaat cagcatttat caaaacttat
    26101 atccaggagt aatacaaaaa tgcagaaaat tattccggtt agtgcttttg atacagatgg
    26161 ttataccgaa tgattaccag caatgacatt cacgaggagt gatgattcgg cagcatttaa
    26221 gggcatatca gaaaaagaga aggacatgaa agaggagagc aataaaagac acggaaaata
    26281 aagaagagag agaaaatatt ttttaaaaac agaaaattaa caacgcacat caggaaacgt
    26341 cgtctcctga ttcagtgata ccacatgaaa atttagtgaa cggttaccgt tctgaagtct
    26401 atgtcagaag atattccatt accaaaaatc aatcagcgat acagagacga tcatggcgcg
    26461 cttgtcaccg tgaccagtgt tgaggaaacc cgggtggtgt ttatgcggga cggctatccg
    26521 catccctgca tgcggccgat gtacaacttc ctgggaaaat tcaaacccga accccggaaa
    26581 gaaccccctg ccggcaatca cactgcctga tggcagcata atccacttat taattctcat
    26641 ccccggagac aagcccagaa aaccactccg ggtgttcact gtatgccgtg ttcagcgcat
    26701 cccaggaaac gttgatataa aagctgtcct gcaatttgcg ggtaacaatt tctgccaccc
    26761 ggttacggtt atccgcctgt ccggcataac ctctctctgc cagccactca ttcagttcat
    26821 gttccggaga cagattgata atatcgtaat cggaccttgc cattcccgtt tccttagcac
    26881 atgttttcag gctcggggat aaagaatgtc gtactgctgc tgccagagca atattccccc
    26941 tgatggtcga atcagagata gtaggccggt actttgcggg ttgttcccgt cagattatca
    27001 tgcacctccg cggtcgttgt caccaccggc attccgctct ttctcaacct caggacatca
    27061 gccgcaatac gctgactgcc gaaccagaac aggccgtcca gtgcagtcac cagcaacccc
    27121 gcctccagcg catgcttcag ccgttcacgg ggcgctttca cttcccgggc aatctgctgg
    27181 tatggcgatg atgtgttttc attcccaatc acccggcgaa tacgatgaga cagatgatac
    27241 cggtatgtat ccggcacacc ggaaaggctg gccttcaggc tgtacacgca gccaaagcgt
    27301 ttatcgttga acaccacatt tttctggctg atatcccatt cttcacgcag cgcggcaatc
    27361 agttgtggtg tccgggtaag caacaagcga aaaggcagtt caaaactggt gacgtaatcc
    27421 acattcagca gtgcaatacg cagccgttct tccggcccgg cctctgtctg ccggcactcc
    27481 tccagaacat cctgccactg ctggtgaagg cgtggaagtt cattcagttc agtaaaactg
    27541 tatttatccg caagatagtc aattcgtgta cgcatactga aaagaattcc gtataaagat
    27601 tcagctggca aaactttatc agtctgtaaa aactaacgga agagtcgata tttctcccgt
    27661 ccatcaccgg atgattgttg caataccccg tggcatcaga gactgaacag ccgtttttaa
    27721 cgccacgtat tgccctgatg aaccaggccg ggcaacccga aaacatcttt ccacccggca
    27781 ttgtccgcca gcacttatca ccgaccaggt ctgttgcagt aaatccacca gttgcgcaca
    27841 ttcttcatca gccatgacgc cgactcgcgg atggcaaata ctcgtctggc tgagcagcaa
    27901 cagacatcgt atctgtgctc cttcatgttg ttcccgcaac cagcgtaata ccaccggcgc
    27961 tgatgggctg accgtgtgat tatgctccgt aatacgttcg tgtacccgtc ggtgaaagga
    28021 actacaaaat gtctgaatct gttgaccata gattccttct gccgaataga gcgtgaagtg
    28081 aaatgccaac agatttggcc agtggtccac cgcctgctga acaaaacgcc ggatttcccc
    28141 cggctctgaa attaaggctt cggttatttg cacgaaatgt tccttttcaa cccctgttga
    28201 attttgtcaa gccggtgcag acacagtaaa gcgcg
//
LOCUS       DAZKHK010000064        28152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKHK010000064 DAZKHK010000000
VERSION     DAZKHK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.064"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..351
                     /gene="yhcN"
                     /locus_tag="ACHOS5_002758"
     CDS             88..351
                     /gene="yhcN"
                     /locus_tag="ACHOS5_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP9777550.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(406..678)
                     /gene="yhcO"
                     /locus_tag="ACHOS5_002759"
     CDS             complement(406..678)
                     /gene="yhcO"
                     /locus_tag="ACHOS5_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP9777551.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(770..2737)
                     /gene="aaeB"
                     /locus_tag="ACHOS5_002760"
     CDS             complement(770..2737)
                     /gene="aaeB"
                     /locus_tag="ACHOS5_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP9777552.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(2743..3675)
                     /gene="aaeA"
                     /locus_tag="ACHOS5_002761"
     CDS             complement(2743..3675)
                     /gene="aaeA"
                     /locus_tag="ACHOS5_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP9777553.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLTKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3683..3886)
                     /gene="aaeX"
                     /locus_tag="ACHOS5_002762"
     CDS             complement(3683..3886)
                     /gene="aaeX"
                     /locus_tag="ACHOS5_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP9777554.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4069..4998
                     /gene="aaeR"
                     /locus_tag="ACHOS5_002763"
     CDS             4069..4998
                     /gene="aaeR"
                     /locus_tag="ACHOS5_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP9777555.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5126..6571)
                     /gene="tldD"
                     /locus_tag="ACHOS5_002764"
     CDS             complement(5126..6571)
                     /gene="tldD"
                     /locus_tag="ACHOS5_002764"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP9777556.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6727..10527)
                     /gene="yhdP"
                     /locus_tag="ACHOS5_002765"
     CDS             complement(6727..10527)
                     /gene="yhdP"
                     /locus_tag="ACHOS5_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP9777557.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGFLPAAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFARLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10595..12064)
                     /gene="rng"
                     /locus_tag="ACHOS5_002766"
     CDS             complement(10595..12064)
                     /gene="rng"
                     /locus_tag="ACHOS5_002766"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP9777558.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12054..12647)
                     /gene="yhdE"
                     /locus_tag="ACHOS5_002767"
     CDS             complement(12054..12647)
                     /gene="yhdE"
                     /locus_tag="ACHOS5_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP9777559.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12656..13144)
                     /gene="mreD"
                     /locus_tag="ACHOS5_002768"
     CDS             complement(12656..13144)
                     /gene="mreD"
                     /locus_tag="ACHOS5_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9777560.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13144..14247)
                     /gene="mreC"
                     /locus_tag="ACHOS5_002769"
     CDS             complement(13144..14247)
                     /gene="mreC"
                     /locus_tag="ACHOS5_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9777561.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARASGGQ"
     gene            complement(14313..15356)
                     /gene="mreB"
                     /locus_tag="ACHOS5_002770"
     CDS             complement(14313..15356)
                     /gene="mreB"
                     /locus_tag="ACHOS5_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP9777562.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15661..17601)
                     /gene="csrD"
                     /locus_tag="ACHOS5_002771"
     CDS             complement(15661..17601)
                     /gene="csrD"
                     /locus_tag="ACHOS5_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP9777563.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17753..18727
                     /gene="yhdH"
                     /locus_tag="ACHOS5_002772"
     CDS             17753..18727
                     /gene="yhdH"
                     /locus_tag="ACHOS5_002772"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP9777564.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTSMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18913..19300
                     /locus_tag="ACHOS5_002773"
                     /pseudo
     CDS             18913..19300
                     /locus_tag="ACHOS5_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19705..20175
                     /gene="accB"
                     /locus_tag="ACHOS5_002774"
     CDS             19705..20175
                     /gene="accB"
                     /locus_tag="ACHOS5_002774"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9777565.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20186..21535
                     /gene="accC"
                     /locus_tag="ACHOS5_002775"
     CDS             20186..21535
                     /gene="accC"
                     /locus_tag="ACHOS5_002775"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9777566.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(21627..22673)
                     /locus_tag="ACHOS5_002776"
     CDS             complement(21627..22673)
                     /locus_tag="ACHOS5_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9777567.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSITPRTL
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(22670..23632)
                     /locus_tag="ACHOS5_002777"
     CDS             complement(22670..23632)
                     /locus_tag="ACHOS5_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9777568.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            complement(23654..24643)
                     /locus_tag="ACHOS5_002778"
     CDS             complement(23654..24643)
                     /locus_tag="ACHOS5_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9777569.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(24644..26143)
                     /locus_tag="ACHOS5_002779"
     CDS             complement(24644..26143)
                     /locus_tag="ACHOS5_002779"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9777570.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVIRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(26204..27094)
                     /locus_tag="ACHOS5_002780"
     CDS             complement(26204..27094)
                     /locus_tag="ACHOS5_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9777571.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(27130..27984)
                     /locus_tag="ACHOS5_002781"
     CDS             complement(27130..27984)
                     /locus_tag="ACHOS5_002781"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFP9777572.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQLVEIPLVLHGASGLSEQDV
                     QLCIRHGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         7075 a         7536 c         6690 g         6851 t
ORIGIN      
        1 ttagttcaat aattagtgta tacttgattt tgtgatatgg gtcacgaaac aaaggcccag
       61 ctaaaagatt atgtcgaggt aaaaatcatg aaaatcaaaa ccactgttgc tgcattaagc
      121 gtactttctg ttctctcttt cggtgcattc gccgccgact ccattgatgc tgcacaagca
      181 caaaatcgtg aagcaatcgg gaccgtatcc gtaagtggtg tggcgtcttc gccaatggat
      241 atgcgtgaaa tgctgaataa aaaagcggaa gagaaaggcg caacggccta ccagattact
      301 gaagctcgta gtggtgacac ctggcacgct acggctgaac tgtacaaata aaccctcatc
      361 gtcttgtccg acgatattgc ccccggttcg ggggcttttt ttgcgctaat gacgaacatt
      421 aaaacgcaaa tgcccttcca gctcttcctc tgcctcatca aacagcaata ttaacgcgcc
      481 aaaacggcga cgcgttttct ctcccagatg aacaaattca atctcaagtg gtagcggcag
      541 gacatcgttc attaacacat cccatagtga gtcgagatcg cgtaccttat ctttcgccag
      601 accaaaggtt tggctaaagt cacgataaaa atcctcctga ctctcaatct catcaaaatc
      661 aaaggtataa atattcatct gttgccaccg tcacgtttcg cggcgagttc tgccgttcat
      721 cgctaagtat agccatgaaa aaaccgacgc ttcgggcgcc ggttttgact taactatcgg
      781 tcaacgcatg ttgatactta tggagcatac ccgtaagccg atgtactggt tccgtcacct
      841 gcggtggcgc ttgccagatg cgtagctttt cctggtagat ttccagttct tccagcaact
      901 ggccaaagta ccgacgacgt ttatcatcgc tacgggcaga tatcacatgg tctgctgtgc
      961 ggcgcatttg ccggtgaaac gccgataaat cctcgttaac cggaatcggc gcatcacgca
     1021 ggcgctggtg cgcgataatc atcgtcagcg ccaggcgaaa tttcggcaaa tcccccggga
     1081 acttattcat cagcaaaaac agctgctgat aaagtgccgg gaggtgattc tctttacgac
     1141 gtgccacatt ggtagtcatc gcggaaacag cggcagaaac aaactgatta agcagtacac
     1201 gtccagtcct gtcgcgcgat ttatcccgca ccagcaaaat aacggtgaac gcgagcacac
     1261 agccgacgat ttgccctaat gcgctgtcga gaaactgact gaaatggaaa gtcatcgggt
     1321 tatccagcac gataatgttt atggtgctgg ccaacgcccc catcgagccc aatctccgtt
     1381 tctgtacttc tataccgagg aagaatccca gcactgccag gctaatacac agcagcaaca
     1441 tgctctgttg ggtattaggg ataatcacca aaaagtagag cagccctaac ggtagcgctg
     1501 ccagcgtccc gtagataaag tcgatcgcca ccatgcgtgg attcggcaaa cgcattgcca
     1561 gtgacgtcac taccgcaatc atcaccattg caccactgcc ggaagtccag cccgtccaca
     1621 gccagaaaag cgtgcccaga atgcaagaaa gtgtggttcg ccagaagtta accattgcat
     1681 gatgacgttc ggctgactct acttttactt ccggttcgcc ttgcaggatc tcttcttcgg
     1741 tggcgttgat ttttgtgtta ctgataacgc cgcgcttgag aagctgataa cgcgttgccg
     1801 ccgcgaccca gctataaatg gtgacaggcg tttcccgttc cccggtccag gcgatcactc
     1861 tccgcaggcg tttgagctgc ttgtggacgt cctgcgcagt ttctaccggt gtgtcaaaaa
     1921 attcgcggaa agtatcagtg atcaattccg ggcgcgtatt ctgaataaga taagtttcgc
     1981 aggattgggt aatcagcgtc agcgatagcg tattgatcgc ttttaaacgt cgattggccc
     2041 gcgcccagcg ggaagattcc atattcaggt tgctgcgcat accttgtaac gccgtagtac
     2101 gtcgcaccag atcgccccag gctttatcga caacttcacc atcgccatgc ttgatacaga
     2161 gttgcattaa ttgatattgc gcaaccagca aactttccag ctctcgatcc acttcttgtt
     2221 tgatcgatcg cggagaaaag agcaaatccg ccataattgc acacacaata ccgataacga
     2281 tctcgctaca acgttcgacg gcaaactgcg gcgtaagcaa tggttccggc tgaatagtga
     2341 tcacaatgat cagcgcggta taaccggcca gcccccacgc atacgagttt tctattcgta
     2401 ccagcgagga tatccaggta caaaaaccgg cccagataca gcacaccaga atcatcaata
     2461 atggtgctcg gatcatcgca atgatgatta ccagtccggc aatacagcca ataaatgtgc
     2521 cgatgatgcg caaaaagcca cgatagcgaa tagcgccaga atacggttca cctcccgcag
     2581 caaaggccgg accggcggca acaatcgccg ctgtcagtac cgcccagcgt ggcgtttcca
     2641 gctggaagtg aaagccaaca aacagcgcca gtacaatggc ggtcgccagt tttaccgcaa
     2701 agcgaatatg ttgattagca atggagaaaa tacccatcgt gattaaccaa actcacgcag
     2761 gcgatgagcc attttacgga agaacgaatc ctgactttcg tcgcgatctt gtttgccagt
     2821 gactaccacc gtagcagtgg tgcccgcagg ccagatgttc tcttgctggt tgtcgagacg
     2881 aatacgtacc ggaacacgtt gcgccaaacg tacccattca aggttagagt cgatggtcgc
     2941 catccctttg tcgtcacgcg tgctgctggc attggtgacc cctgcggcaa cactatcaac
     3001 agtccctttc agcactttgt tactgccaag cggcgtgatc tccgcacgat acccagggcg
     3061 caccccttcc agcttagttt cttccatata ggccagtaca tagaaggagt tctgtttcac
     3121 cagcgcaacc gccgttgatc ctcgagtaat aaactcaccg gtatagacgt tgaggttggt
     3181 cacccagcca tctgctggcg cgcggatgac tgtacgttca agatccagtt ttgccagatc
     3241 gcgggtcgcc tgcgctttcg ttaactgatg cagaactgtt tgtagtacgt tgttggcctg
     3301 gtcgatctct tcgcgagaca tcgcctgcac accgagacgg ttacgacgtc cggcctcctg
     3361 gcgtttctcc tgcgccagta cctgataata agcaacatcg gcttgcgctt cctcaagcgc
     3421 cttttgataa cgcggctggt cgatggtgaa cagtacctgt ccttttttca ccagctggtt
     3481 atcatgaaca ttcacctggg taatgagtcc agaaacgtcc ggcgcgatcg caacgacgtc
     3541 agcgctaaag cgcgcgtcac gcgtccacgg ggattcggtg taatagaccc aggcattaaa
     3601 aattgcgatg aaggccagaa tgactaatac gaccgtgatg gccgtacggg agaattttct
     3661 tattagtgtt ttcacttcaa cctcaaacga acagtcgcga tatcaaataa aacaagcagc
     3721 aatagagcgc ggtgttgaac aacgccggat gccagacaaa gtcgtagata cctgttggca
     3781 caagtacccg gcgcaccagc cagaaaatcg ccagtgataa aagcaattcg aaaaatatcg
     3841 gtgggaagga cagcccaaac accacgataa cgggaaacag actcatgttg accttggttg
     3901 tatagagaga gcaggcgtta ttattttcag catctgtcgc cgcagagaag ggcatggaaa
     3961 gccgggcgag agcaacattg ctgtagattg atatttaata tattaacgta actgttatgc
     4021 tgttatctat attatgtgat ctaaatcact tttaagtcag agtgaataat ggaacgacta
     4081 aaacgcatgt cggtgtttgc caaagtagtt gaatttggct cttttaccgc cgccgccaga
     4141 cagctacaga tgagcgtttc gtccatcagt cagacggtgt caaaactgga agatgagttg
     4201 caggtaaagc tgttaaaccg tagcacacgc agcattggcc tgaccgaagc cggtagaatt
     4261 tactaccagg gctgccgtcg gatgctccat gaagtgcagg atgttcatga gcaactgtat
     4321 gccttcaata acacccccat cgggacgctg cgcattggct gttcttcaac tatggcacaa
     4381 aatgttctcg ccgggctgac ggcaaaaatg ctgaaagaat acccaggttt gagcgtcaat
     4441 ctggttaccg gaattcctgc ccccgacctg attgccgacg gtctggatgt ggtgatccgc
     4501 gtcggcgcgt tgcaagattc cagcctgttt tcccgccgtc tgggcgcgat gccaatggtg
     4561 gtgtgcgccg caaaaagcta tctcacacaa tacggcatac cagaaaaacc cgccgattta
     4621 agtagtcact catggcttga atacagcgtg cgacccgaca atgaatttga actgatcgca
     4681 ccggaaggga tctcgactcg cctgatcccg caagggagat ttgtgactaa tgatccgatg
     4741 acgttggtgc gctggctgac tgcaggcgct gggatcgcct acgtgccgtt gatgtgggtg
     4801 atcaacgaga tcaatcgcgg ggaactggag atcctgctgc cacgttacca gtcagaccca
     4861 cgcccggttt atgcgttata taccgaaaaa gataagctgc cgctgaaggt acaagtcgtg
     4921 atcaactcgc taacggatta ttttgttgag gtcggtaaat tgtttcagga gatgcacggg
     4981 cgcgggaaag agaagtaatt aatattgtac tgttacgttg tacaaacctg tgccaacggg
     5041 ttcccctcac cctaacccca gaggggcgag gggaccgttc gtacacggag aaggattaat
     5101 tatccttctg aaaatactga aattattacg cagtaccgcc aacagtcagg ttatcaactt
     5161 tcagcgttgg ctggcccacg ccaaccggca aactttgccc ttctttaccg cagacaccca
     5221 cgccgttatc cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg gtttcgatac
     5281 cggaaccaat caacgttgcg cctttcaccg gcttagttac tttaccgttt tcaataagat
     5341 acgcttctga ggtggagaaa acgaatttgc cggaggtgat atccacctgg ccgccaccaa
     5401 agttcggtgc atagataccg tactcaacgg attcaataat ttcctgcggg gtcgatttac
     5461 ccggcagcat ataggtgttg gtcatacgcg gcatgggcag atgagcgtag gattcacggc
     5521 gaccgttgcc agtcggcgtc atccccatca aacgcgcgtt gagtttatcc tgcatgtagc
     5581 ctttcagaat gccgttctca atcagcacgt tgtactggcc tggcgtacct tcgtcatcaa
     5641 tcgccaccga accacggcga tcgaccatcg tgccatcatc aaccacggta cacagttctg
     5701 aagccaccaa ctccccgacc tgtccactaa atactgaagt gccacggcgg ttgaagtcgc
     5761 cttccagacc gtgacccacc gcttcatgca acagcacgcc cggccaacct gcgccaagta
     5821 ctaccggcat ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc gccatacgca
     5881 ctgcttcttt tgcccacgca tccgcacgga cttcgccgtc gagatcagca aggaagaact
     5941 cataaccaaa acgaccgccg ccaccactgg caccgcgttc gcgtttgcca tcttcttcga
     6001 ccaaaacgct cacggaaaga cgcaccagcg gacgaacatc cgccgctagt gtgccgtcgg
     6061 tggccgcaac cagaattaat tcatagacac cactgaggct ggcagtcact tcctgtacgc
     6121 gcttgtccgc ttcgcgggca accttatcga cgcgacgcag aatatccaac ttctcttcac
     6181 ggctcatgct ttgcagcgga tctaccgagg tatataacgg gctatgctct accgcgccca
     6241 gcgtctgtac tttgccatca ccactatcac ggacgatggt gcgcgccgct tgtgcactct
     6301 gttccagcgc cagcaggctg atttggtcag cataagcaaa tccggttttt tcaccactga
     6361 ttgcacgcac gccaacgccc tgatcgatgt tgtaagaacc atctttaata atacggtctt
     6421 ctaaaaccca ggattcgtga tagctcgact gaaaatagag atcgccataa tcaaggcgac
     6481 gttcggccag ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg ttcgccgcta
     6541 gcaattgttc acttaccagg ttcagactca tcgtttttgc tactcgttag ttactgcagt
     6601 agaggatttt ttacggctgc cggatgcggc gtgaacgccc tgtccggcct acggttatgt
     6661 tctggctcgt tggcctttgg caacgattat catatgagat tggggcaatt acgcgccctc
     6721 gtcaaatcat tgcgcttttt ctttgcgcgg ttgtcgcaac acttcgttga tttgcggatc
     6781 gtccagcgga cccgaaatgt gatagcgcaa aatggagact ttgctccaca gcggtcccag
     6841 cactttactg gcggcaaaca ctgccgcgcc aacaatgggg ttaaccgcaa aggccgcagc
     6901 cacgccgacc gtcgcagaaa tctctggtgc gacaaccgct tccatattca ggtcgcgacg
     6961 taccagattt accgaccctt tcatagcgat gtccgcttcc aggccatcca ccagcgtgtc
     7021 gtcggtgtgc ataacgccgt ctttaatcca cgcggtgctg cgaatggagt caaaatagaa
     7081 cccttcgcca aaagtgtctc tgaaatcaaa acgcagctta cgcatcaggg catctacgct
     7141 caataagcgc agcaattgcc cggcatgtcc ggtattgatt tcggtaattt cgcctttacc
     7201 cagttgagta tgaatgatgc cattcaacgt cgcctcatct ggctgccagg gtgctttgcg
     7261 ccagtgtaaa tcgtaatcca cattaaatga cgactggcgt atgggcgtcg tgacaccaaa
     7321 aaattctgcg gcggcatcaa ttttctgccc gcgcagtttt cctttcagcg aggttcgttc
     7381 attccccgga ttgttaaccc attcaccatc ggcagtgagc cgcgcaaaac cagtatcaat
     7441 cagtccatta gtcagcgtta acgtatcgcc agaaatggtg atatcactgt caatgcgacc
     7501 gaatttttgc ccccagaacc agcactctgc gcatcgtatt tgtgcgtctg gccagccacg
     7561 gaagttaatg cgttccgttg tcgggaaagg tgatgactgt gttgaatcac cacgagtttt
     7621 cgccacgctt gggttgtaat aaagatattt gatattcgcc agccacggtg cgttattacg
     7681 catcgccagc gtggcgttga tttcacgccc ttgcgcctca accagggtgc catttgccgt
     7741 cggttgcgaa acaatactca ggttattcca ttgctgattt cccagcgaca acataggcgt
     7801 acgtaacgtt atgtgttgtg ggaaactcgc tgcaccaccg acactctccg ccgcgccttt
     7861 ctggaacagg gccagccact cggcaccatt catcggcggc atattgagtt caacgccact
     7921 ttgttccggc aacggcggga gcgttttact gtctgccgcc caaatagcac gatcgagcgt
     7981 cagcttttga ccgagcaacc agcggctatt gaaatggtta tccgcaccag cctgtccggt
     8041 taattcaaag ctgttgagat tgccatcaac cttaacgttt accggtagtg gttcacccgc
     8101 aggtttggct aacggtgaag gtaagtgact gctcacattc ttcaaatcgc cgttcagctc
     8161 tacgttatac gtcgcgccag catgataagg cagctcaatg cccactttac catcccacgc
     8221 cacgctgcca cttaatgctt cgttcaccgc tgcaggcaga aagccggttt tcgctggttg
     8281 ccagttaccg ttgagattca ccgcgacctg gtaggctttt gccccttctt tggtggaaaa
     8341 atccacgttc aacggctgat taaaccagct tgctgtcagt ggttcacttt gcagatcgcc
     8401 attgataaag ctgaatttac cgctcaaatt tttcagggtg ctgtcgagtg gtttgataaa
     8461 cagactgtta ttacgcagcg tcacttcacc tttcgcggtt accaactcgc cgttcagcgg
     8521 gatatcaaga tgtaagcgag cattcacatc gccgtcgagc tggagttctt gcagggtcgc
     8581 acccagggaa tctttcagcg gtgtctcatc aaagtaaggg ccaacggctt tacccggacc
     8641 tttaatgtca gcgtcaatca gcagtttttc ttttgagtag tcagggatca ctgcggtgag
     8701 attactcgcg cgcacgccgc ccagattaac gccatcggtt ttcatccata aaccgtcgtt
     8761 aataaagtcc agttcaatat caaggttagt taatgcaggc cagtccggct ggaaggcaaa
     8821 cttcgcgttg cgcagcggca ccagcacttc aaactgacct tcgttgtgtt tataggggaa
     8881 gagttgcgga ttgccaccat aaaccagcgt cgcgttatcc gcttcaccgc cctgaatcgc
     8941 gccacttaag taatcaacca ggtctttacc catcaagttt tccgggaaat agcgccaggc
     9001 ttgtgaacca tcatcggtac tgatgccagc cagaataccc agccagggtt catcgttagc
     9061 aggttgcagg taacgaaaac cgccgcgcgc atggacggct ttggctttaa cgtcaatatt
     9121 acgcccatcc agctggaaac ctttgtcatt gttcagccag cttatcgttg cctggccgtc
     9181 ggcgatttcc agtggcgcac ggaataccgt ttcgtaaggc atctttgcct gcttcatcga
     9241 cgcagtaagc aaaccatttt ctacgctgcc ggaaagcgtc ccggagaagt gttccgcacc
     9301 cggtaataat ttccattgct tccaggccag atcgctccac gatgcctgaa aacgggtctt
     9361 gtctgccgcc tgaagcggga tatccagcgc cagagtgtta atcttgccgc tcggttgtgt
     9421 ggagcgccaa acatcaccca gtgcaggtga aagtttcgcg accagcgggc gtacgccctc
     9481 caggcctgcc agctccagat tactggcacg aatccggagt tcgtcactgc gtttattgtc
     9541 tttgccgcca acgtcctgtt ccggtatcca ggccagcgtc aatgctccgc tcggccaggg
     9601 tttgccgtcc atcgtgatcc gtgtatcggg aatagagaac tgccatcccg gattttcacg
     9661 cgtaatatgc gcagtcagat tatccaccga cagcgtatgc gtttccttct cgcccaacca
     9721 gctggcaccg ccctgtttca gccagacgtc accgccggtt acatcgcctt tgtcgatcgt
     9781 catccagcct tcaagggaga actgtgccgt ttccagcgca atattgtcct gcatccattt
     9841 accgagccac ggcttcaggt cgatgtcatc cgcctggagc cagacgcgac cattgcttaa
     9901 caacccctca tcatcgcgca aatccatacg cacctgcatc acgccgtgct gtccggtaag
     9961 gctggagagg cttaccaggc cttccgcacg gtgtcgacgt ggatcgttca gccaggtgag
    10021 ctgtgggatc gccagctcgg cgcgctgacc ggatggcgtc aggaaactga cttcactgtc
    10081 gcggagatcg aaatggtcaa attgacgaag aaacagatcg ctgatgtgac tggcttccag
    10141 gctatcatta ccaccgccgc tggtgatagg agtgttggtg cgaaaacgca gctgccagaa
    10201 agtgaggtcg cgaaactgcc agcgcatatg taacaggctc tgccagacat ccagcgccag
    10261 agtaacgcgt ttaaccgaaa attcgccgcc atcttttagt tctgcacgga tgtcgtgcgc
    10321 ttcaagcgtc ggaccaaaat tctgccagct ggctgagagc tgactggctt ctaccggcat
    10381 gccagtcgcg gattctattt tgttgaggat ttccggacgc caggcgtcaa gatgcggtaa
    10441 agcaatacgc aggccgctaa ccagcagggc agcgatcaca acgagcgcgg ctccagtaag
    10501 cagtaatatc cccggcaatc gcctcacccg tcactccttg tctgctaaaa atgtgactca
    10561 aaaaaccttt gccggatggc ggcccagcat ctgtttacat cattacgacg tcaaactgct
    10621 cctggttata gagcggttca atttgtactt taacctgttt accaacgaaa atttccactt
    10681 ccgccagcga gtgtgactct tcgcctttca aggcttcagc tactgccgga gaagcataga
    10741 ccaggaaacg gtcggaatca taagcatggt ggacacgaac aatctcgcgc atgatttcat
    10801 agcataccgt ttccacggtt ttcaccgttc cgcgaccgtg gcaggttggg cattcgttac
    10861 atagtacgtg ctcaatgctt tcgcgggtgc gtttacgcgt catctccacc agccccagcg
    10921 ccgaaaaacc attaacgctg gttttcaccc ggtctttgct caacgcctgc tccagcgaat
    10981 gcaacacgcg gcgacggtga tcttcattat tcatgtcgat gaaatcaata atgataatcc
    11041 cgcccagatt gcgcaaccgt aactggcgag cgatagcctg cgtcgcttca atattggtat
    11101 tgaaaatggt gtcgtccaga ttgcgatgac cgacaaacgc cccggtattg atgtccacgg
    11161 tggtcatcgc ttcggtctgg tcgataatca gataaccacc ggatttcagt tctactttgc
    11221 gttccagcgc tcgctgaatt tcgttttcga catcaaagag atcgaaaatc ggctggcgtc
    11281 cggtgtaatg ctccagcttg ctggtcatct cgggaatgta ctccgaggta aactccagca
    11341 atgcttcgta agtcaggcgt gagtcaacgc gaatgcggtc gagttcggcg tcggcgaaat
    11401 cacgcagaac acgctgcgcc agcgccagtt cgccgtacag ctgataacgg gtctgcgggc
    11461 gttttttacg ctccataact ttggtccaga cgcgtttcag ataagcggca tcggaggcca
    11521 gttcagcctc gccaacccct tcagctgcgg tacggatgat aaacccgccc tgctcgtcgc
    11581 aatactctgc gaccactttt ttcaggcgtt cacgttctga ttcgctttca attcgttggg
    11641 ataccccaac gtgagaagcc cctggcataa acaccagata gcgagaaggc agcgtgatat
    11701 cggtggtcag gcgcgcacct ttagtgccaa gcggatcttt caccacctgc accatcagat
    11761 cttgcccctg acggaccagt tccgagatgt cgcgcaccgt gaattgcttt tgttcttcac
    11821 ccgccacaca ttcagtgtgc ggcatgatgt cagatgcatg aagaaacgcg gctttatcca
    11881 gcccaatatc tacaaaagcc gcctgcatac ccggaagtac acgacttaca cgacccttgt
    11941 agatattgcc tactattccg cgtcgcgcct cacgttcaat atgaatttcc tgcagaatac
    12001 cgccatcaat atacgccact cgagtttccg aaggcgttac gtttaccaac aattcagccg
    12061 tcatgtttat cccttttctc acgcagtgcg ttaaaattac ttaataattc atacgtttca
    12121 accagcggta agccgactac ggcgtgaaag ctgccattta tcttcctgac aaaacagcca
    12181 cccagcccct gaataccgta tgcacctgct ttatctaacg gttcgccgct ggcgacatag
    12241 cccgcgatgt cttcgtctgt taacgttctg aaagtcacat cggtgaccac caggcaatcg
    12301 agaatgtgct ggctgtcggc caacgccact gccgtcatca cctgatgggt ctgacccgat
    12361 aatttgcgca acatctgcgc cgcatgctct gcgtcgcgcg gtttctcgag cacttctccg
    12421 ttcaggataa cgatagtatc cgcacccagc accgggagat cctgcgccgt ttgcgcgaca
    12481 cccgcctgtg ctttctcgcg cgccagacgc acaacatact gctgcgcgct ctcctgcggc
    12541 tgacgctgct cctcaatgcc cgtaacaata cgttcaaagg tcacgccaag ttgcgcaagt
    12601 aactcctgac gacgcggaga accggaagct aaatacagag aagtcataga aacctttatt
    12661 gcactgcaaa ctgctgacgg actttacgca tcagcaagaa aatccacggc cagagaacgc
    12721 cattgactac actactccag aacacttccg gtctgaaaga gacgttaatc actaaaaact
    12781 ctgcccagaa aacaataata tccaccacca gcgaaagcaa catgacgacc agcgcctgct
    12841 gccataatgc gaggttgcgg aaaagctggt atttcagcgc caccaggtaa gcaatgatgc
    12901 tcatcgccaa tacgcgtacg ccaagcgtcg agccgctgat cagatccagt atggcaccca
    12961 tcacaaaacc tgtgcccaca tttacgcgat ggggcaaggc caggatccaa tacaacaaga
    13021 tgagtaacac ccagtttggc cggaaaacaa tcaggttatc cggccagggc atgatctgca
    13081 gcaacagcgc aatgaggaaa gagagccaga ttacccagcg tccctggcta cgatagctcg
    13141 ccactattgt cctcccgacg cacgcgcagg cggttgagca ccactttgcg gcggcgtagc
    13201 cctttgtgga gagcgattag ctgcgggctg tgtgggcgca gcaggcgcag taactgcatt
    13261 tcctgtcgcc ggttgctgcg gagtcggctg agtgatcccc gttgccggtt caggtaactt
    13321 tggccccatc gcgtctggcg aaggcaatac ctgcggcatc atctgcatca gacgttcatt
    13381 ggcaacacga tgcacctctt ccggcgtcat cgggttagcg ccgttacgat ccgcgcccca
    13441 cagcagcagc agataacgca aacgttgcag tcctgcagtc ggacgcgcct gaatcacagt
    13501 ataagcgcgc tgggtatcga gttttacgga agagacaacc gcgaccggat agccttccgg
    13561 gaaacgaccg cccagaccgg aagtcaccag cacatcacca acacgaatat ccgtattcgc
    13621 tggcagatgc tcaagctgca aatcatccgt acaaccgtta ccggctgcaa ttacgcggat
    13681 atcgttgcgc agcacctgga ttggcagcgc gtgggtcgca tcacaaatca gcagcacgcg
    13741 gctggtcagt ttagcaacgg ccaccacctg accaacaaca cctttgtcgc tgatgacagg
    13801 ctggccttca tacacgccat taacactacc tttgtcgata acgacttgat cgctataagg
    13861 atcgttaacc gtggagataa cctgagtcac cattttctgc tcgtcctgac gcagcggaga
    13921 acccagcagt tcgcgcagac gcgcgttctc ctgtttgtat tgtccaagca tcagcagttc
    13981 actgtttttc agcagcagtt cctgacgtaa cgcccggttt tcaagttcta gttggtcacg
    14041 cgaggccagc gtctgcgata cgccatccag caattcacga ggagcattgg aaacaaagta
    14101 gaaaggactg acggcggtat ccatataagt acggatttga ctgaacgtcc ccaggcggct
    14161 gtcggcaata ataatgccga gcgccaccag caccgccaga ataaggcgaa tctgtagcga
    14221 cgggccacgg ctaaaaattg gcttcataag ttatgcgtat tctcgtatca gaccaggcag
    14281 ggtaaacaga cacttcccct gcctgcatcc gattactctt cgctgaacag gtcgccgccg
    14341 tgcatgtcga tcatttccag cgctttgccg ccaccgcgcg ccacacaggt cagcgggtct
    14401 tcagcaacaa cgactggaat gccggtttct tccattaaca aacggtcaag gttacgcagc
    14461 agtgcgccac caccggtgag caccatgccg cgctcggaga tatcggaagc cagttccggc
    14521 gggcactgtt ccagtgcaac cattaccgcg ctcacaatac cggtcagcgg ttcctgcagt
    14581 gcttcgagga tttcgttgga gttcagggta aaaccgcgtg gaacaccttc tgcaaggtta
    14641 cggccacgaa cttcgatttc acggacttca tcgcccgggt aagccgaacc gatttcgtgc
    14701 ttgatacgtt ctgcggtggc ttcaccgatc agagaaccgt aattacgacg tacatagttg
    14761 atgatagctt cgtcgaaacg gtcaccacca atgcgcacgg aagaggagta aaccacgccg
    14821 ttcaaggaga taacagcaac ttcagtggta ccaccaccga tatcaaccac catagaaccg
    14881 gtcgcttcag aaaccggcag gccagcacca attgcggcag ccatcggttc ttcaatcagg
    14941 aagacttcac gggcaccagc gccctgcgcg gattcacgaa ttgcgcggcg ttcaacctgg
    15001 gtcgcgccaa ccggcacaca aaccagaacg cgcgggcttg gacgcataaa gctgttgctg
    15061 tgcacttgtt tgatgaagtg ctggagcatt ttttcagtca cgaagaagtc ggcgataacg
    15121 ccgtctttca ttgggcgaat ggcagcaata ttgcccggcg tacggcccag catctgcttc
    15181 gcgtcatgac ctactgcagc tacgcttttc ggtgaaccgg cacgatcctg acgaatggcc
    15241 accacggaag gctcattcaa tacgatgcct tgtcctttta cataaatgag ggtattcgca
    15301 gtacccaggt caatggacaa gtcattggaa aacatgccac gaaatttttt caacatacta
    15361 agggataatc ctgaaagctg gggcggaaaa gaaaatccgc ttactttacc aaccacacgc
    15421 agcagcgaca aggcgcaaaa atcatctgct acggtgaaaa ttagtgcagt tcgtttcctt
    15481 tgttacaaat ctctgcctga gtccagaaag gcttaatgca tcagcagcat tcctcgcctg
    15541 tttgcaaccg cgtaaggtca ttcatctgca tatgtgctgc aacaatctgg cgagcagaca
    15601 agcacactcc catgagacgc agcgcgcgtt attctacgtg aaaacggatt aaacggcagg
    15661 ttaaaccgag tatctttgtg aatatttttt cacgttagta tcaagtggct gtgaggacgc
    15721 gaaaaaatcc ccttgcccac ctgtaacacc gcgctgaatc agggtctgcc actcgcttcg
    15781 cgaacgtacg ccggtggcgt aaacctgggt gctggtcccg gagcaggctt ccaccaggct
    15841 ttgaaccagc agctggttct ccgttcgctt ctcaatgttt ctgactagcc ccggatggag
    15901 cttgagtaac tcaacattaa gttctttgat ccaactggtg cttaccagcg tcaaaccagc
    15961 ctggttgacg gctacccgta cccctaaagc attcactaaa cgaataacag gttgtaagcg
    16021 actgatatgt tgaccaacat cggcctctgc aagttcaata attatgcgtc ttcgttgcga
    16081 tttttcacat tgcattaacg tatcgcgcag ccaacgctga aaacgcgggc gaatcagcga
    16141 ctcaacggta acctgaatcg ccagattttc ctctggccag taacgcaata gtggaataag
    16201 acggctgatt tgcaggcggt catactcttc cgataaacca aactgcaaga ccatcggcat
    16261 atactctgcc gagctaacct cttcattacc atcgaagatg cggcacatga gttcacgatg
    16321 atgaacctgg ccttcgcgag taaccgccgg tttttgataa aggcgcgggc cgccgcgact
    16381 gagcatttgc tcgataagcg tacgccagcg aacattaccg cgtccttttt caggcaacga
    16441 gtcatcgtaa atagcccagc tattgccgcc ctgcaatccc gcattacgcg tggcagactc
    16501 tgcatgttcc attacctgct cggtatcctg accactacgc caggcacaga taccaatgtg
    16561 gatcatatcg tcgcgatcga gcattttatt gttcggcaag gtatcgacgg ctttgattaa
    16621 ctgactggcg atgctctctg cctcttttaa cgtccggtgc ggtaacagcg cagcaaaatc
    16681 actgcggtgg taacgcgcca gcagtgcgcc agggtagcgc atcataaatg tcgatagcag
    16741 attcgtcaga gagaagaact gttcttcaac ctggctgtgc ccccaggtat cactcaacat
    16801 attgaaatcc ggcagacgaa tcatcatcac gatcccgtgg gtaccaactt tctcttgatc
    16861 ttccagtaac gttgctaact gattatcgaa aaagagtcgg ttattgaggc cggttttcat
    16921 gtcctgggcg gcataagagc ggatcagcgt atcaagacgg ctgtgttgtt cgcgtgcgtt
    16981 ctgaatttca caaagcagcg tatccagcgc actgctggtt ctgggcggcc attcatagat
    17041 ggttcccaac acattagagc cacgctcacc gtttaagata cgagtagccc gggtttccag
    17101 caattcttgc ccggcaagtt gccgttgtaa ccagcgtacc gccaggaaga gcataagaat
    17161 gataaagcca atcgcccccg tgagcggcgc ggtagtcatt aaagaatgga aatagttgcc
    17221 catcggatcc tgataaacca gacgcagcga catccccgga tgctttatca acggaacgct
    17281 cagttcacga aacagatcgt tggtgccaac cggacgataa ctaccatttc tggccagggt
    17341 ataaacctgc ttatcaccat ggagcaggtc tacacgaacg atatctgccg acatcattaa
    17401 ttcggtaatt tgtggcctta atgtgctgaa gtcattcgac actaggtggg tatcaatcgc
    17461 cgtcgccacc gcctgaacac gatgactaaa cttatactga atggcgttgt agaaacttag
    17521 cgaacagccc agcaaagtca caaaaattgt taacccggtg agcagcgtga caaaggccga
    17581 aaatttcgtc gttaatctca tccttgtgtt aactccgata gtgaggaagc gggcatacta
    17641 gcaaatcaga tttatctcgc aatttattgc gcttcatcgg ctttgctttt ccattagcga
    17701 gtatagtctt cagaaattat tttccaatcc atcatgcaca tgaggaccac ttatgcaggc
    17761 gttactttta gaacagcagg acggcaaaac tctcgcatca gtacagactc tggacgaaag
    17821 tcgcctgccg gaaggcgatg tcacggttga tgttcactgg tcgagcctga actacaaaga
    17881 tgcgctggcg attaccggta agggaaaaat catccgtaat tttccgatga ttcctgggat
    17941 cgattttgcc ggaactgtac gcaccagcga agatccgcgt tttcatgccg gtcaggaggt
    18001 gttactcact ggctggggcg ttggtgaaaa ccactggggt gggctggcgg aacaggcgcg
    18061 agtgaaaggt gactggctgg ttgccatgcc gcaagggctg gacgcgcgta aagcaatgat
    18121 tatcggtact gccggtttta cctccatgct gtgtgtgatg gcgctggaag atgccggtgt
    18181 tcgcccgcag gatggggaga ttgtcgtgac gggtgccagt ggtggcgtcg gcagtaccgc
    18241 cgtggcgctg ctgcataagt tgggttatca ggtcgttgct gtttccggtc gcgaaagtac
    18301 ccatgaatat ctgaaaagtt taggtgccag ccgtattctt cctcgcgatg agtttgctga
    18361 atcccgtcct ctggaaaaac aagtctgggc tggggcaatt gacaccgttg gcgacaaagt
    18421 gctggcaaaa gtgctggcgc aaatgaatta cggcggctgc gtggcggcct gtggtctggc
    18481 gggtggtttt actctgccaa ccacggtcat gccatttatt ctgcgtaatg tccgtttgca
    18541 aggggtggat tcagtaatga cgccaccagc acgccgcgca caagcctggc agcgactggt
    18601 cgccgattta ccggaatcat tctataccca ggcggcaaaa gagatatctc tggcagaggc
    18661 accgaagttt gccgaggcca tcattaataa ccagatccag ggtcgcacgc tggtgaaggt
    18721 taactaacca tttagcaggg aataataaga gagggaactc attttgaaat cattattcaa
    18781 tcggttaacg ggaaaagcgg ttagccggac agctttcgtc gaacatcttg gtcatgaagt
    18841 tgtacaacat catccaaact ggaaagtcat gatttcgact gaccataaat tgatgcgcat
    18901 tgatgctcca ccaaacagcc attattgata cgcctccgtc gcctgttagg tttatggtgt
    18961 tttgccttgg cgacgctacg cttaatctgt tactgatttc tggtaccatg gggggaataa
    19021 tctgatttta tttgattgca aattaaccac ttaaacctat tgaatgcgga acattgtcag
    19081 tcggttaatt ttgcgtgctt tagcattcac atctttccgg acgatgcagt gaaaattggg
    19141 caatccccag caaccgctgc gtaatgtcgt ctatcttgtc gcgatcctgg catccctaca
    19201 ttatttgtgg tctgtgaaga ttatctcatt gcagcccccc atcttcgcag ggctggcttt
    19261 acagcttttc accttacgtt ataagaagtt ccgtcgatga tggcgctaat ttcgtgaatt
    19321 gtgcggcttg ttgcaaatta cacggtgttg aaggttattt acatgttagc tgttgattat
    19381 cttccctgat aagaccagta tttagctgcc aattgctacg aaatcgttat aatgtgcgac
    19441 ctcgtcctcc ctgacgcagt ttttgcgctg cggaaaaggt gacattggcg caacgaaggt
    19501 atattttgtt ttttgccgga ggatagcagc agatcgctgc acaatgtccg tcaagtctaa
    19561 cattgacact ctggggcaaa atagaccggc gtcccggcct gctggaattt atcgctatgc
    19621 atacagctgt cggggcatac gctttacaga cggcggtgaa acgcctgtca caatcacact
    19681 aaacaaagag tacggaaccc actcatggat attcgtaaga ttaaaaaact gatcgagctg
    19741 gttgaagaat caggcatctc cgaactggaa atttctgaag gcgaagagtc agtacgcatt
    19801 agccgtgcag ctcctgccgc aagtttccct gtgatgcaac aagcttacgc tgcacctatg
    19861 atgcagcaac cagctcaatc taacgcagcc gctccggcga ccgttccttc catggaagcg
    19921 ccagcagcag cggaaatcag tggtcacatc gtacgttccc cgatggttgg tactttctac
    19981 cgcaccccaa gcccggacgc aaaagcgttc atcgaagtgg gtcagaaagt caacgtgggc
    20041 gataccctgt gcatcgttga agccatgaaa atgatgaacc agatcgaagc ggacaaatcc
    20101 ggtaccgtga aagcaattct ggtcgaaagt ggacaaccgg tagaatttga cgagccgctg
    20161 gtcgtcatcg agtaacgagg cgaacatgct ggataaaatt gttattgcca accgcggcga
    20221 gattgcattg cgtattcttc gtgcctgtaa agaactgggc atcaagactg tcgctgtgca
    20281 ctccagtgcg gatcgcgatc taaaacacgt attactggca gatgaaacgg tctgtatcgg
    20341 ccctgctccg tcagtaaaaa gttatctgaa catcccggca atcatcagcg ccgctgaaat
    20401 caccggcgca gtagcaatcc atccgggtta cggcttcctc tccgagaacg ccaattttgc
    20461 cgagcaggtt gaacgctccg gctttatctt catcggcccg aaagcagaaa ccattcgcct
    20521 gatgggcgac aaagtatccg caatcgcggc gatgaaaaaa gcaggcgttc cttgcgtacc
    20581 gggttctgac ggcccgctgg gcgacgatat ggataaaaac cgcgccattg ctaaacgcat
    20641 tggttatccg gtaattatca aagcctccgg cggcggcggc ggtcgcggta tgcgcgtagt
    20701 gcgcggcgac gctgaactgg cacaatccat ctccatgacc cgtgcggaag cgaaagctgc
    20761 tttcagcaac gatatggttt acatggagaa atacctggaa aatcctcgcc acgtcgagat
    20821 tcaggtactg gctgacggtc agggcaacgc tatctatctg gcggaacgtg actgctccat
    20881 gcagcgccgc caccagaaag tggtcgaaga agcaccggct ccgggcatta ccccggaact
    20941 gcgtcgctac atcggcgaac gttgcgctaa agcgtgtgtt gatatcggct atcgcggtgc
    21001 aggtactttc gagttcctgt tcgaaaacgg cgagttctat ttcatcgaaa tgaacacccg
    21061 tattcaggta gaacacccgg ttactgaaat gatcaccggc gttgacctga tcaaagaaca
    21121 gctgcgtatc gctgccggtc aaccgctgtc gatcaagcaa gaagaagttc acgttcgcgg
    21181 ccatgcggtg gaatgccgta tcaacgccga agatccgaac accttcctgc caagtccggg
    21241 caaaatcacc cgtttccacg cacctggtgg ttttggcgta cgttgggagt ctcatatcta
    21301 cgcgggctac accgtaccgc cgtactatga ctcaatgatc ggtaagctga tttgctacgg
    21361 tgaaaaccgt gacgtggcga ttgctcgcat gaagaatgcg ctgcaggagc tgatcatcga
    21421 cggtatcaaa accaacgttg atctgcagat ccgcatcatg aatgacgaga acttccagca
    21481 tggtggcact aacatccact atctggagaa aaaactcggt cttcaggaaa aataatttgt
    21541 gacgcgcggt gttttcatcg cgcttagcaa agaacgtaat caacgccggg cctgttaatg
    21601 ctacccggcg tttttattgc cgttaactaa cggtagcctg caatttccgc tttcaacgcg
    21661 ctaatttcag catcgccatc aacaattccc agctcaaagc agtatttctt tgtttcccct
    21721 ggctcaataa agggcagacg cccctgcgct cgttctttat cacgaccgtc aggaaaacta
    21781 ttactgacct ccagtcccat aacatattca cccgcgccta aatttttcca ctggataaag
    21841 ttgggtagct gcatggtgtc gaatttctcg taaattcccc accccagttc ggcgttcacc
    21901 agcgcaactt tactcatccc ctgactgtcg ctattcaacg tgagatagta aacctgctcg
    21961 tcaaaaccgg gctgtggggt acaaatctcc agatgactgg ttaacccttc cgccgcatgc
    22021 agagttcgtg gcgttatgct tttagccgac agcaagattt ctgcctgtgg cgataataaa
    22081 ggccagccgt aattaaagtg ataaagaatt tgcagcggct ggcgggtgaa accatgatta
    22141 gtcaccttgt cttcaatgcg cattacattt tcgccataac ggcagcgaat ggtcctttcc
    22201 agcgtcagat tttcaccgaa caatcgtgtt tcccgtactt tgccattgat gacaaattca
    22261 atgctgtcgt cggtccgttc tgtgcgatac cccacctctt ctgctggagt cgcagccaaa
    22321 cgcccgtgta accccagcgt ttctccttca tcctcacagg gcgtgccaat gtagctaagt
    22381 ccgcaggtag tcagtaaccc cgcgtaaaag ctacgtaaaa acccgttgcc ctgagactca
    22441 aaaaaagcgg gggcgacaac accgttgggg gtgataaaag agagcgcagt gtccttgtag
    22501 ctggcccatg caatatccag cgcgcgatct tttaccaccg taaatgccag tcccgcacca
    22561 gtacggacat caaacgcttc tgtacccttt gctctgccac tacgtaactc atagtgttta
    22621 attccggcaa cctgcgagag atcgccaatc cacgaccaaa tttcgttttt cattgtgctg
    22681 cttcccgaat ggacatgtca tggcgctgga tgaacgtttc gatttccata aggcgagacg
    22741 ttccctccat tgggccacgt cgggttaccg ccagtgcacc gcaggcgttg gcatattgta
    22801 atgcccgatg ggcgtcaaac cctagctggc ggcaggcaat ccacgcgcca ccaaagcagt
    22861 ctcctgcacc ggtcgggtca acttcttcca ccggatagct ctcaacatga aactgctcat
    22921 tcgcggagta gtaactggct ccttgattgc cgcgcttcac aatcacctct ttaacacctt
    22981 cttcaagaaa accagcgatt gcacgctccg gcgtcgagtg tggcgacaag agcaacactt
    23041 ccccctcact gggcatatag atatccgtta actcaagaac aaagtggagg gcatcgcgca
    23101 tttctggaat atcaagcatt tctttacgaa tattcgggtc gaaagaaata acgccaccat
    23161 ttgctttaac gatggttact gcttttttta ctgcgtctac catatggaat gaaaacaggg
    23221 atgaacccat aatatgaaaa tgggtacaat ccttcagaat attttcatca acatgctgtg
    23281 ccgataattt accgcaagcg gcattcttga tattaaaaat aaaatcgcga tcgccagagt
    23341 tatgataagt aacaaaggca ctaccggtgg cttccagagg taagactgaa atccccctga
    23401 tgtccacacc gtcagcagcc agacgatgta tattaatatc accaaagcca tcattcccca
    23461 cgcagctaat gatgccgcac gggacgccaa gacgtgtcac ctggtcaata aaaatagcgg
    23521 gcgcaccact gggataaggg ccgttccaga tacctggttg atcaaatggc tggccgattt
    23581 tactcgccat aatttcaacc agaatttctc caattgtgaa gaccttactc atatgattaa
    23641 ttccttgaaa tgattagctg cgttttcttt tatttcttac gtcaaaataa acggctacaa
    23701 gaatgactaa tcctttaacc accatctggt aaaaataagg aacattcatt aagttcatgc
    23761 cattattaat aacgccgatg gttaatgcgc cgatcaccgt ccccaccacg gttccatagc
    23821 cgccgttcag gctggtgcct cccagtacag ctgcggcaat agcatccagt tcgtagctca
    23881 acgcggaatt cggttgcccg gaatagaggc gtgaggcaag gatcatcccg ctgatacctg
    23941 ccagcacggc tgtaatcata aagactttaa ttttcaagcg ggtgacattt atccctgaat
    24001 aaattgctgc ttctttatta ccaccagtga tataaatttt tcgtccaaag gttgttttag
    24061 taagaatgaa gtggtttatc agcaataaga tgattaatat ccatatagga ataggtatat
    24121 agagaaattc tccattcccc agcgccagaa aggcatcatc tttaatcatg atgggcatac
    24181 catcggtcac tatataagcg ataccacgga agatccccat agtagcaacg gtgacaataa
    24241 acgaagggat ccccgctata gccgtcaagc caccatttat catccccaga gccagtgctg
    24301 caataatgga taatgggacc aataaaacaa atggaattcc ccatgaagcc ccaagcgcag
    24361 caaatgtgcc aatcaatgca atggttgaac cgaccgataa atcaatatcg ccaagcaaaa
    24421 gcacataagt cattccatag gcggtaattg caattatcga aacctgaaga ataatattag
    24481 taatgttatt ggcggtaaaa aaactgtcat taataaaact gaacagaatg attaacacca
    24541 ccattccact gaggatacca ccatattgcc tgaagacttt tttaaaatta tttgcaatgg
    24601 gaatttccga aggtttcgga ctggcaagag ttttctctgt catttaacgt actcctgttg
    24661 cagcggtcat tacctgtgtc tgcgttaaat tttcacatga ataaatatcg gcaatcgttt
    24721 tattacgtat taccaccaga cgatcgctaa gtgccatgac ttctggcagt tcagaggaca
    24781 ccagaataat tgctgtccct gccgcagcca gttgacgtat cacgttatag atttcaaatt
    24841 ttgcgccgac atccactccg cgcgttggct catcgagcaa taaaatacgc ggagttttct
    24901 ccagccactt cgataacacg attttctgtt gattaccgcc gctcagtgct cccactgcct
    24961 gatgaacaga actcacttta gtgttcattc tggcaattga ttgcagcgtc ttcgcctctt
    25021 cttgtcggtg attgatgagt cccatccgcg aagcgttata ttgaaacgcc acatggtgcg
    25081 tgttcatatt gacgtcgtgc atcagcacta acccttcttc cttgcggttt tcggtcacaa
    25141 aagcaatgcc atgatcaata gcctgtttgg gattagcgat cctgacttcc ttaccatcaa
    25201 tcaacacagt cccgtgatac gaaacgaggc caaatagcgc cttcatcaca tcggaacgcc
    25261 cggcccccac caggccaaaa aatccgagga cttcccccgg acgcacagcg aaactgacct
    25321 ctttaaaaag cgaaggatgg gaaagatttt ttacttccag ttttgcagga acattttcat
    25381 ccggtttttc cccaagacgt gctggccaaa tatttttcat ttcccgacca accatttggg
    25441 caatcaagtg ctggatatca ctgtttttct tttcatgcgt agcgatatac tcaccgtcgc
    25501 ggatcaccga aatatcatct gaaatccgca taatttcatc catgcgatga gagatataaa
    25561 taaccgcaca gcctcgctgc tttaattttt caataatgtt aaacaggatc tccgcttcat
    25621 tatcacttag cgaagatgtc ggttcatcca taatcacgac atccgctgca tagcttaagg
    25681 ctttggcaat ttctaccagt tgcctatgtg ccatcgacat attgccgacc atttccagag
    25741 ggtctagtgg gatgcccagt tccgcgagga ggttctggca atctgcgagc atttttttat
    25801 cgttaatgat gccaaaacgt cgcggttcat tattggcata aatattttcc gccacggtca
    25861 tattattact caggctcagt tcctgaaaaa taatcgcaat ccctaactgg cgagaatgct
    25921 gcggattatt aatggtgatg gtttcaccac gtagtgttat cgttccctgg gttggcggat
    25981 aaacaccggc cagtattttc attaatgtcg atttaccggc accattctcg cctaataaag
    26041 tatgcacccg ccctttacgt aaagagagtg taatatcttt taatgcctga acatgaccaa
    26101 atgtcttcgc aatatgcgaa acatcaagaa taacatccgt cataatgacc tcggtgtcac
    26161 aacaaaacgc caataacgtt ttgttgtgag gtgaagatat ttactattta cctgtataaa
    26221 cgccgggttg taccgggatc attttttcta ccggtttacc attgaggtga tcaacagcgg
    26281 catcaatggc tttagccccc atttgatcgg gatattgccg aatcacggcc gcaaacgttt
    26341 tttcactgtc aacggcatca cgcgcttctt tcatgccgtc aaaaccaatg attttgatgt
    26401 tgtcgttatg cgcagatttt gctgcgataa ccgctgctag cgccgcatca tcaccaaagc
    26461 caaacagacc attcaggttg gcgtttgcct gcataatgtt ttgcgcagtc gttaacgctt
    26521 cagcccgcgt aataccaggc tgttcagcaa caattttaat atccggtgta tcgcttaacc
    26581 ctttcttaaa accatcaacg cgcgcaacca ccgattgtag tgccggataa gaaataatgg
    26641 ctacagtacc tttaccatgc aaaagtttac ccattaactt gcctgcttca acgcctcccg
    26701 catagttatc ggtcgcaaca tgcgatacga ccggaacgcc ttccgcagcg acgtcgacgg
    26761 tgataacggg aataccggcc ttatcggctt taattatcgc tgcctggacg cctttcgaat
    26821 cgaccggaga gataataatg gcatccactt ttttagtaat aaaatcctca acatcagaaa
    26881 gttgtttgtt taaatcctga ttggcaatag agacacttaa attgacattg tctttttttg
    26941 cctcttcttt catggcattc gcaagatcaa tatagaaagg atgttgttgt gttaataatg
    27001 acgcaccaat tttatactct tttgccaacg aactttgcga gaacaatgcc aggctcacca
    27061 acatagctga accaagtaat ttaatatttt tcataaacaa tcctcattat ttttgtaggg
    27121 tacaacgtct taaatttttc cttcactacc acagacgcca ataatacgag ttacgacttc
    27181 tttcatggcg gcctttgcgg gctgcatata gtggcgagga tcatttgccg caggattgtt
    27241 gtagaaatat ccttttaacg catcggcaaa cgcaattttc aattctgtcg cgacatttac
    27301 tttacaaata ccgtggcgaa tacagagctg aacgtcctgt tccgataggc ctgaagctcc
    27361 atgcaacacc agcggaattt ctaccagctg gcgaatttgt tccagccgca caaaatccag
    27421 acgtggttca tgcgcatata aaccatgtgc agtgcctatt gctaccgcca gagagtcaat
    27481 ttgggtacgt tgaacaaact ctgcagctgc ggctggtgag gtataagccg aatctttttc
    27541 atccaccagc agatcgtcct cctggccgcc cagacgcccc agttcagcct cgacgctgac
    27601 atcgaagcga tggcaaaagg tggtaacttc ttgcactcgc gcaatgttgt cttcaaatgg
    27661 caaatgggag ccatcaatca tgacagaacg gatccctgcg cgaaccttat taaaaatgtc
    27721 atcactctct tcatgatggt cgagatgcag gactaacggc aggttgtact cgcgagccag
    27781 cgattgtact aacgaaatta aacttcccac gccaccataa cgatatgtac cgggtgttcc
    27841 cgccagcata acgggagagc gaagttgtgc agcgctctca acaactacct gtaaagtttc
    27901 aaggttatga atgttgaaag cagggacggc atatccctct gactgggctt ttttcagcat
    27961 gtttttgctt gaaataatat acatcgcagc tcctgttatt tgtcgttcgc cttacaaaat
    28021 gaagaaagag aatcttcact ttcattttga aagcaaacta gcacagcaaa agcagcaaaa
    28081 agcgtgatgc atgtcgaaca tcatcaaaat aacttacaaa aaataactta atttattttt
    28141 cactttgcaa gt
//
LOCUS       DAZKHK010000065        28128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKHK010000065 DAZKHK010000000
VERSION     DAZKHK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.065"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            101..871
                     /gene="fixA"
                     /locus_tag="ACHOS5_002782"
     CDS             101..871
                     /gene="fixA"
                     /locus_tag="ACHOS5_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP9777573.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            886..1827
                     /gene="fixB"
                     /locus_tag="ACHOS5_002783"
     CDS             886..1827
                     /gene="fixB"
                     /locus_tag="ACHOS5_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9777574.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            1878..3164
                     /gene="fixC"
                     /locus_tag="ACHOS5_002784"
     CDS             1878..3164
                     /gene="fixC"
                     /locus_tag="ACHOS5_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9777575.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            3161..3448
                     /gene="fixX"
                     /locus_tag="ACHOS5_002785"
     CDS             3161..3448
                     /gene="fixX"
                     /locus_tag="ACHOS5_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP9777576.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            3507..4838
                     /gene="yaaU"
                     /locus_tag="ACHOS5_002786"
     CDS             3507..4838
                     /gene="yaaU"
                     /locus_tag="ACHOS5_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9777577.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            4946..5476
                     /gene="kefF"
                     /locus_tag="ACHOS5_002787"
     CDS             4946..5476
                     /gene="kefF"
                     /locus_tag="ACHOS5_002787"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP9777578.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            5469..7331
                     /gene="kefC"
                     /locus_tag="ACHOS5_002788"
     CDS             5469..7331
                     /gene="kefC"
                     /locus_tag="ACHOS5_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP9777579.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVADAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            7523..8002
                     /gene="folA"
                     /locus_tag="ACHOS5_002789"
     CDS             7523..8002
                     /gene="folA"
                     /locus_tag="ACHOS5_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP9777580.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            8088..8321
                     /locus_tag="ACHOS5_002790"
     CDS             8088..8321
                     /locus_tag="ACHOS5_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP9777581.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            8324..8638
                     /locus_tag="ACHOS5_002791"
     CDS             8324..8638
                     /locus_tag="ACHOS5_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFP9777582.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIVGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(8635..9483)
                     /gene="apaH"
                     /locus_tag="ACHOS5_002792"
     CDS             complement(8635..9483)
                     /gene="apaH"
                     /locus_tag="ACHOS5_002792"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP9777583.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(9490..9867)
                     /gene="apaG"
                     /locus_tag="ACHOS5_002793"
     CDS             complement(9490..9867)
                     /gene="apaG"
                     /locus_tag="ACHOS5_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP9777584.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            complement(9870..10691)
                     /gene="rsmA"
                     /locus_tag="ACHOS5_002794"
     CDS             complement(9870..10691)
                     /gene="rsmA"
                     /locus_tag="ACHOS5_002794"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9777585.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            complement(10688..11677)
                     /gene="pdxA"
                     /locus_tag="ACHOS5_002795"
     CDS             complement(10688..11677)
                     /gene="pdxA"
                     /locus_tag="ACHOS5_002795"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP9777586.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            complement(11677..12963)
                     /gene="surA"
                     /locus_tag="ACHOS5_002796"
     CDS             complement(11677..12963)
                     /gene="surA"
                     /locus_tag="ACHOS5_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP9777587.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            complement(13016..15370)
                     /gene="lptD"
                     /locus_tag="ACHOS5_002797"
     CDS             complement(13016..15370)
                     /gene="lptD"
                     /locus_tag="ACHOS5_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP9777588.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNIATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            15625..16440
                     /gene="djlA"
                     /locus_tag="ACHOS5_002798"
     CDS             15625..16440
                     /gene="djlA"
                     /locus_tag="ACHOS5_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP9777589.1"
                     /translation="MQYWGKIIGVTVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            complement(16558..17217)
                     /gene="rluA"
                     /locus_tag="ACHOS5_002799"
     CDS             complement(16558..17217)
                     /gene="rluA"
                     /locus_tag="ACHOS5_002799"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP9777590.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(17229..20135)
                     /gene="rapA"
                     /locus_tag="ACHOS5_002800"
     CDS             complement(17229..20135)
                     /gene="rapA"
                     /locus_tag="ACHOS5_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP9777591.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAMDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTSSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(20300..22651)
                     /gene="polB"
                     /locus_tag="ACHOS5_002801"
     CDS             complement(20300..22651)
                     /gene="polB"
                     /locus_tag="ACHOS5_002801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP9777592.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASTLDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALAIYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDTQL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(22726..23421)
                     /gene="araD"
                     /locus_tag="ACHOS5_002802"
     CDS             complement(22726..23421)
                     /gene="araD"
                     /locus_tag="ACHOS5_002802"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9777593.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(23706..25208)
                     /gene="araA"
                     /locus_tag="ACHOS5_002803"
     CDS             complement(23706..25208)
                     /gene="araA"
                     /locus_tag="ACHOS5_002803"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9777594.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(25219..26919)
                     /gene="araB"
                     /locus_tag="ACHOS5_002804"
     CDS             complement(25219..26919)
                     /gene="araB"
                     /locus_tag="ACHOS5_002804"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP9777595.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESIVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQNVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            27258..28103
                     /gene="araC"
                     /locus_tag="ACHOS5_002805"
     CDS             27258..28103
                     /gene="araC"
                     /locus_tag="ACHOS5_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP9777596.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT         6223 a         7349 c         7807 g         6749 t
ORIGIN      
        1 tattggtgat taaagtttta tttccaaatt aaagggcgtg atatctgtaa ttaacaccac
       61 cgatatgaac gacgtttcct tcatgatttc tggagatgca atgaagatta ttacttgcta
      121 taagtgcgtg cctgatgaac aggatattgc ggtcaataat gctgatggtt cgttagactt
      181 cagcaaagcc gatgccaaaa taagccaata cgatctcaac gctattgaag cggcttgcca
      241 gctaaagcaa caggcagcag aggcgcaggt gacggcctta agtgtgggcg gtaaagccct
      301 gaccaacgcc aaagggcgta aagatgtgct atcgcgcggt ccggatgaac tgattgtggt
      361 gattgatgac cagttcgagc aggcactgcc gcaacaaacg gcgagcgtac ttgctgcagc
      421 cgctcagaaa gcaggctttg atttgatcct ctgtggcgat ggttcttccg acctttatgc
      481 ccagcaggtt ggtttgctgg taggcgaaat ccttaatatt ccggcagtta acggcgtcag
      541 caaaattatc tccctgactg cagataccct caccgttgag cgcgaactgg aagatgaaac
      601 agaaacctta agcattccgc tgcctgcggt tgttgctgtt tccactgata tcaactcccc
      661 acaaattcct tcgatgaaag ccattctcgg cgcggcgaaa aagccagtcc aggtatggtc
      721 ggcggcggat atcggtttta acgcagaggc agcctggtca gaacaacagg ttgctgcgcc
      781 gaaacagcgc gagcgtcagc gcatcgtgat tgaaggcgac ggcgaagaac agatcgccgc
      841 atttgctgaa aatcttcgca aagtcattta attacagggg atgctatgaa cacgttttct
      901 caagtctggg tattcagcga taccccttct cgtctgccgg aactgatgaa cggtgcgcag
      961 gctttagcta atcaaatcaa cacgttcgtc ctcaatgatg ccgacggcgc acaggcaatc
     1021 cagctcggcg ctaatcatgt ctggaaatta aacggcaaac cggacgagcg gatgatcgaa
     1081 gattacgccg gtgtcatggc tgacactatt cgccagcacg gcgcagacgg tctggtgctg
     1141 ttgccaaaca cccgtcgcgg caaattactg gcggcgaaac tgggttaccg ccttaacgcg
     1201 gcggtgtcta acgatgccag cgctgttagc gtgcaggacg gtaaagcgac agtgaaacac
     1261 atggtttacg gcggtctggc gattggcgaa gaacgcattg ccacgccgta tgcggtactg
     1321 accatcagca gcggcacgtt cgatgtggct cagccagacg cgtcacgcac tggcgaaacg
     1381 cacaccgtgg agtggcaggc tccggctgtg gcgattaccc gcacggcaac ccaggcgcgt
     1441 cagagcaaca gcgtcgatct cgacaaagcc cgcctggtgg tcagcgttgg tcgcggtatt
     1501 ggcagcaaag agaacattgc gctggcagaa cagctttgca aggcgatagg tgcggagttg
     1561 gcctgttctc gtccggtggc ggaaaacgaa aaatggatgg agcacgaacg ctatgtcggt
     1621 atctccaacc tgatgctgaa acctgaactg tacctggcgg tggggatctc cgggcagatc
     1681 cagcacatgg ttggcgctaa tgcgtcgcaa accattttcg ccatcaacaa agataaaaat
     1741 gcgccgatct tccagtacgc ggattacggc attgttggcg acgccgtgaa gatccttccg
     1801 gcactgaccg cagctttagc gcgttgatcc actctggcag ggctgcattt tggccctgcc
     1861 gctgacaggg agctcttatg tccgaagata tctttgacgc catcatcgtc ggtgcagggc
     1921 ttgccggttc ggttgccgca ctggtgctcg cccgcgaagg tgcgcaagtg ttagttatcg
     1981 agcgaggcaa ttccgctggt gccaagaacg tcaccggcgg gcgtctctat gcccacagtc
     2041 tggaacacat tattcctggt ttcgctgact ccgcccccgt agaacgcctg atcacccatg
     2101 aaaaacttgc gtttatgacg gagaagtcag cgatgactat ggactactgc aatggcgacg
     2161 aaacctcgcc atcccagcgt tcttactccg ttttgcgcag taaatttgat gcctggctga
     2221 tggagcaggc cgaagaagcg ggcgcgcagt taattaccgg gatccgcgtc gataacctcg
     2281 tacagcgcga tggcaaagtc gtcggtgtag aagccgatgg cgatgtgatt gaagcgaaaa
     2341 cggtgatcct tgctgatggg gtgaattcca tccttgccga aaagctgggg atggcaaaac
     2401 gcgttaaacc gacggatgtg gcggttggcg taaaggaact gatcgagtta ccgaagtcgg
     2461 ttatcgaaga ccgttttcag ttgcagggta atcagggcgc ggcttgcctg tttgcgggat
     2521 cacccaccga tggcctgatg ggcggtggct tcctttatac caatgaaaat accctgtcgc
     2581 tggggctggt ttgtggtttg catcatctgc atgacgcaaa aaaatcggtg ccgcaaatgc
     2641 tggaagattt caaacaacat ccggccgttg caccgctgat cgcgggcggc aagctggtgg
     2701 aatattccgc tcatgtagtg ccggaagcag gtatcaacat gctgccggag ttggttggtg
     2761 acggcgtatt gattgccggt gatgctgccg gaatgtgtat gaacctcggt tttaccattc
     2821 gcggtatgga tctggcgatt gccgccgggg aagcagcagc aaaaactgtg ctttcagcga
     2881 tgaaaagcga cgatttcagt aagcaaaaac tcgcggaata tcgtcaacat cttgagagtg
     2941 gcccgctgcg cgatatgcgc atgtaccaga aactaccggc gttccttgat aacccacgca
     3001 tgtttagcgg ctacccggaa ctggcggtgg gcgtggcgcg tgacctgttc accattgacg
     3061 gcagtgcgcc ggaactgatg cgcaagaaaa tcctccgcca cggcaagaaa gtgggcttca
     3121 tcaatctgat caaggatggc atgaaaggag tgaccgtttt atgacttctc ccgtcaatgt
     3181 ggacgtcaaa ctgggcgtca ataaattcaa tgtcgatgaa gagcatccgc acattgttgt
     3241 gaaggccgat gcagataaac aggccctgga gctgctggtg aaagcgtgcc ccgcaggtct
     3301 gtacaagaag caggatgacg gcagtgtgcg cttcgattac gccggatgtc tggagtgcgg
     3361 cacctgtcgc attctggggc tggggtcggc gctggaacag tgggaatacc cgcgcggcac
     3421 ctttggtgtg gagttccgtt acggctgatg ttggtttgat acgtaatgcc gtactgactc
     3481 tcattgcaaa aaaacaggaa taacccatgc aaccgtccag aaactttgat gatcttaaat
     3541 tctcctctat tcaccgccgc attttgctgt ggggaagcgg tggtccgttt ctggatggtt
     3601 atgtactggt aatgattggc gtggcgctgg agcaactgac tccggcgctg aaactggacg
     3661 ctgactggat tggcttgctg ggcgcgggaa cgctcgccgg gctgttcgtt ggcacatcgc
     3721 tgtttggcta tatctccgat aaagtcggac ggcgcaaaat gttcctcatt gatatcatcg
     3781 ccatcggcgt gatatcggtg gcgacgatgt ttgtctcatc ccccgtcgaa ctgttggtga
     3841 tgcgggtact tatcggcatt gttatcggtg cggattatcc catcgccacc tcaatgatca
     3901 ccgagttctc cagtacccgt cagcgggcgt tttccatcag ctttatcgcc gcgatgtggt
     3961 atgtcggcgc gacctgcgcc gatttggtcg gctattggct ttatgatgtg gaaggcggct
     4021 ggcgctggat gctgggtagc gcagcgattc cctgtttatt gattttgatt ggtcgattcg
     4081 aactgcctga atctccccgc tggttgttac gcaaagggcg agtaaaagag tgcgaagaga
     4141 tgatgataaa actgtttggc gaaccggtgg ctttcgagga agagcagccg cagcaaaccc
     4201 gttttcgcga tctgtttaat cgccgccatt ttccttttgt tctgtttgtt gccgccatct
     4261 ggacctgcca ggtgatccca atgttcgcca tttacacctt tggcccgcaa atcgttggtt
     4321 tgttaggatt gggggttggc aaaaacgcgg cgctggggaa cgtggtgatt agtctgttct
     4381 ttatgctcgg ctgtattccg ccgatgctgt ggctaaacac tgccggacgg cgtccattgt
     4441 tgattggcag ctttgccatg atgacgctgg cgctggcggt tttggggctg atcccggata
     4501 tggggatctg gctggtagtg atggcctttg cggtgtatgc ctttttctct ggtgggccgg
     4561 gtaatttgca gtggctctat cctaatgaac tcttcccgac ggatatccgc gcctctgccg
     4621 tgggcgtgat tatgtcctta agccgtattg gcactattgt atcgacctgg gcactgccga
     4681 tctttatcaa taattacggt atcagtaaca cgatgctaat gggggcgggt atctcgctgt
     4741 ttggcttgtt gatttccgta gcgtttgccc cggagactcg agggatgtca ctggcgcaga
     4801 ccagcaatat gacgatccgc gggcagagaa tggggtaatt tgttcagatt tctctctttt
     4861 ctgaatcaat attattgact ataagctgcg tgaatatatg actacacttt gtgggaaaac
     4921 aaaggcgtaa tcacgcgggc tacctatgat tcttataatt tatgcgcatc catatccgca
     4981 tcattcccat gcgaataaac ggatgcttga acaggcaagg acgctggaag gcgtcgaaat
     5041 tcgctctctt tatcaactct atcctgactt caatatcgac attgccgccg aacaggaggc
     5101 gctgtctcgt gccgatctga tcgtctggca gcacccgatg cagtggtaca gcattcctcc
     5161 gctcctcaaa ctttggatcg ataaagtttt ctcgcacgga tgggcttacg gtcacggcgg
     5221 cacggcgctg cacggcaaac atttgctgtg ggcggtgacg accggcggcg gggaaagcca
     5281 ttttgagatt ggtgcgcatc cgggctttga tgtgctgtcg cagccgctac aggcgacggc
     5341 aatctactgc gggctgaact ggctgccacc gtttgccatg cactgcacct ttatttgtga
     5401 cgacgaaacc ctcgaagggc aggcgcggca ctataagcaa cgtctgctgg aatggcagga
     5461 ggcacatcat ggatagccat acgcttattc aggcgctgat ttatctcggt tcggcggcgc
     5521 tgattgttcc cattgcggta cgtctcggtc tgggatcggt tcttggctac ctgatcgccg
     5581 gctgcatcat tggcccgtgg gggctgcgac tggtggccga tgccgaatct attctgcact
     5641 ttgcagagat tggcgtggtg ctgatgctgt ttattatcgg cctcgaactc gatccacaaa
     5701 ggctgtggaa gctgcgtgcg gcagtgttcg gcggtggcgc attgcagatg gtgatttgcg
     5761 gcggcctgct ggggctgttt tgtatgttac ttgggctgcg ctggcaggtc gcagaattga
     5821 tcggcatgac gctggcgctc tcatcaacgg cgattgccat gcaggcgatg aatgaacgta
     5881 atctgatggt gacgcagatg ggccgcagtg cctttgcggt gctgctgttc caggatatcg
     5941 cggcgatccc gctggtggcg atgatcccgc tactggcagc gagcagtgcc tcgacgacga
     6001 tgggcgcatt tgctctctcg gcgttaaaag tggcgggtgc gctggtgctg gtggtattgc
     6061 tggggcgcta tgtcacgcgt ccggcgctgc gttttgttgc ccgctctggc ttgcgggaag
     6121 tgtttagtgc cgtggcgtta ttcctcgtgt ttggctttgg tttgctgctg gaagaggtcg
     6181 gcttgtcgat ggcgatgggc gcgtttctgg cgggcgtact gctggcaagc tcggaatacc
     6241 gtcatgcgct ggaaagcgat atcgaaccat ttaaaggttt gctgctgggg ctgtttttca
     6301 tcggcgttgg catgtcgata gactttggca cgctgcttga aaatccgttg cgcattgtta
     6361 ttttgctgct cggcttcctc atcatcaaaa tcgccatgct gtggctgatt gcccgaccgt
     6421 tgcaggtgcc aaataaacag cgtcgctggt ttgcggtgtt attaggacag ggcagtgagt
     6481 ttgcctttgt agtatttggt gcggcgcaga tggcgaatgt gctggagcca gagtgggcga
     6541 aatcgctgac cctggcggtg gcactgtcga tggcggcaac gccgattctg ctggtgatcc
     6601 tcaatcgcct tgagcaatct tctactgaag aggcgcgtga agccgatgag atcgacgaag
     6661 aacagccgcg ggtaatcatt gccggattcg ggcgttttgg gcagataacc ggacgtttac
     6721 tgctctccag cggggtgaaa atggtggtgc tcgatcacga cccggaccat atcgaaacgc
     6781 tgcgtaaatt cggtatgaaa gtgttttatg gcgatgccac gcggatggat ttactggaat
     6841 ctgccggagc ggcgaaagcg gaagtgctga ttaacgccat cgacgatccg caaaccaacc
     6901 tgcaactgac ggagatggtg aaagaacatt tcccacattt gcagattatt gcccgcgccc
     6961 gcgatgtcga ccactacatt cgtttgcgtc aggcaggcgt tgaaaagccg gagcgtgaaa
     7021 ccttcgaagg tgcgctgaaa accgggcgtc tggcactgga aagtttaggt ctggggccgt
     7081 atgaagcgcg agaacgtgcc gatgtgttcc gccgctttaa tattcagatg gtggaagaga
     7141 tggcaatggt tgagaacgac accaaagccc gtgcggcggt ctataaacgc accagcgcga
     7201 tgttaagtga gatcattacc gaagaccgtg aacatctgtc attaattcaa cgacatggct
     7261 ggcagggaac cgaagaaggg aaacatactg gcaatatggc ggatgaaccg gaaacgaagc
     7321 cctcatccta ataaagagtg acgtaaatca cgctttacag ctaactgttt gtttttgttt
     7381 cattgtaatg cggcgagtcc agggagagag cgtggactcg ccagcagaat ataaaatttt
     7441 cctcaacatc atcctcgcac cagtcgacga cggtttacgc tttacgtata gtggcgacaa
     7501 ttttttttat cgggaaatct caatgatcag tctgattgcg gcgttagcgg tagatcgcgt
     7561 tatcggcatg gaaaacgcca tgccgtggaa cctgcctgcc gatctcgcct ggtttaaacg
     7621 caacacttta aataaacccg tgattatggg ccgccatacc tgggaatcca tcggtcgtcc
     7681 gttgccagga cgcaaaaata ttatcctcag cagtcaaccg ggtacggacg atcgcgtaac
     7741 gtgggtgaag tcggtggatg aagccattgc ggcgtgtggt gacgtaccag aaatcatggt
     7801 gattggcggc ggtcgcgttt atgagcagtt cctgccgaaa gcgcaaaaac tgtatctgac
     7861 gcatatcgac gcggaagtgg aaggcgacac ccatttcccg gattacgagc cggatgactg
     7921 ggaatcggta ttcagtgaat tccacgacgc cgatgcgcag aactctcaca gctattgctt
     7981 tgagattctg gagcggcggt aatttcgtat attagaattc cggcatcact gatacgtgca
     8041 gatatccgtg tatactagac gtataaattg tacaggagca cgagatcatg actgcaaaac
     8101 gtaccacaca aagtgtgacc gtcaccgtcg accgtgagtt agtcaatcgc gctcgtgatg
     8161 caggcttaaa tatgagcgcc acccttacgg ttgcgctcaa tgctgaactt aaaaaacatg
     8221 cagcaacacg ttggcgtgaa gagaacgcag aagctatcgc tgcgttaaat caattggctg
     8281 atgaaaccgg atgtttctct gatgagtacc gcagcttcta gcgatgcaat ttacggtata
     8341 ccgcagtcgc ggcaggaacg ccgcgtttcc ctttgttatt gatgttacca gcgacattgt
     8401 tggtgagatt aatcgccgta tcgttattcc attaacacct attgaacgat ttagccgtat
     8461 tcgcccacca gaacggctta acccaatcct tttactggta gacggcaaag aatatgtgct
     8521 catgacgcat gagactgcaa ctgttccagt taacgcgctg ggaacgaaat tttgcgatgc
     8581 cagcgcgcac cgaacgttga ttaaaggagc attagatttt atgctcgacg ggatttaaga
     8641 cgctaccgcc tctccctcac ccatatcttt atgccggttc gacggctgga caaaatactg
     8701 tttatcttcc cagcgcaggc aggttaatgt accaccccag cagcagccgg tatccagcgc
     8761 gtaaatacct tccggcgtac ctttgccttc cagcgatgcc cagtgaccaa aggcgatgtt
     8821 gtactcttca gcgacggggc caggaatcgc aaaccacggt ttcagtgggg caggggcctc
     8881 ttccggcgat tctttgctgt acatatccag ttgaccgttc gggaagcaaa aacgcatacg
     8941 ggtaaaggcg ttggtgataa aacgcagtct tcccagcccc cgcaattccg gtgaccagtt
     9001 atttggcata tcgccgtaca tggcatcaag gaagaaggga taggagtcac tcgacagcac
     9061 cgcttctaca tcgcgcgcgc actctttggc ggtctgcaga tcccactgcg gcgtgatccc
     9121 ggcgtgggcc atcaccaact ttttctcttc gtcgatttgc agcaaaggct ggcgacgcag
     9181 ccagttaagc agctcgtcgg catccggcgc ttccagcagc ggtgtcaggc gatctttcgg
     9241 tttattgcgg ctgatcccgg caaataccgc cagcagatgc agatcgtgat tacccagcac
     9301 cagacgtacg ctgtcgccta aggatttcac atagcgcaga acatccagcg aacccgggcc
     9361 gcgcgcgacc agatcgcccg tcagccagag ggtatctttc ccaggggtaa attctacttt
     9421 atgcagcaat gcgatcagtt catcgtaaca accatgaacg tcgccaataa ggtatgtcgc
     9481 catattcttt taatgaatga gtgtgggaac ggcgagtcgg aatacgggaa tgtcgatgct
     9541 gaaagggacg ccattttcat cgatcatttc gtagtgaccc tgcatggtgc ccagcggggt
     9601 ttcaatgatt gcaccgctgg tgtactggta ctcttcgcca ggcgcgataa gtggctggac
     9661 gccaaccact ccttcgccct ggacttcggt ttcacggcca ttgccattgg tgatcagcca
     9721 gtaacgcccc aacaactgca ctggcgctcg ccccagattg cgtatggtta cggtataagc
     9781 aaaaacgtaa cgttcattat caggtgaaga ttgggcctca atgtagacgc tttgaacctg
     9841 aatacacact cggggcgaat tgatcatcgt taactctcct gcaaaggcgc gttctccgcc
     9901 agatagttcg ccatctggca atattgcgcg acagagatat tttccgctcg catcgccggg
     9961 tcgatcccca ttcccgttaa cacctcgacg ctaaacaggt tgccgaggct gttacgaatg
    10021 gttttacgac gctggttaaa ggcttcagtg gtgatgcggc tcaacacgcg aacatcttta
    10081 accgggtgag gcatcgttgc atgaggaacc aggcgcacga cggcggaatc cactttgggt
    10141 ggtggtgtaa aggctgacgg cggtacttcc agcaccggga tcacgttgca atagtattgc
    10201 gccatgacgc ttaatcgacc atacgccttg ctgttcggtc ctgcaaccag acgattcacc
    10261 acttcttttt gcaacataaa gtgcatgtcg gcaatggcat cagtatagct aaacagatgg
    10321 aacatcaacg gcgtggagat gttataaggc aggttgccga aaacacgcag cggctggccc
    10381 attttcgcgg ccagttcgcc aaagttaaag gtcatcgcat cctgctgata aatcgtcagt
    10441 ttcgggccta agaatggatg cgtttgcaga cgtgccgcca gatcgcggtc aagttcgatg
    10501 accgtcaact ggtccagacg ttcgccgacc ggttcggtca atgccgccag accggggccg
    10561 atttcgacca tcgcctggcc cttctgcggg ttaatggcag aaacaatgct gtcgatcacg
    10621 aactgatcgt tgagaaagtt ttgcccgaag cgtttacggg ctaagtggcc ctggtggact
    10681 cgattattca ttgggtgtta acaatcattt tgatggcgag attaagcgcc gtaataaaac
    10741 tgccgacatc ggctttacca cgtcccgcca gttcaagcgc ggtgccgtgg tccactgatg
    10801 tgcgaataaa gggcaggccc agcgtaatgt tcacaccgcg cccgaagccc tggtatttta
    10861 gcacgggaag accctgatcg tggtacatcg ccagcacggc gtcggcgtta tcgagatatt
    10921 tcggctgaaa cagggtatcg gcaggcagcg gcccgttgag tttcatcccc tgttcccgca
    10981 gctcgttgag taccggaata atggtgtcta tctcttccgt acccatatga ccgccttcgc
    11041 ccgcgtgcgg attcagcccg cagaccagaa tgcgcggttc ggcaatacca aatttggtcc
    11101 gcaaatcgtg atgcaaaata gcaatcactt cgtgcaaaag ggctggggtg atagcatctg
    11161 cgatatcgcg cagcggtaaa tgcgtcgttg ccagcgccac gcgaagttct tcggtcgcca
    11221 gcatcatcac cacttttttc gcctgcgaac gctcttcgaa aaactcggta tgaccggtaa
    11281 acggaatgcc tgcgtcgtta atgacgcctt tatgcacggg gcctgtgatc agcgcagcaa
    11341 attcaccgtt cagacagcca tcacacgctc gcgccagcgt ttccaccaca tagtgcccat
    11401 tttcaaccgc taactgcccc gcagtgacag attcacgtag cgcgacagga agtagcgtta
    11461 atgtgcccgc agtttgcggt tgtgcagggg agttggggga ataagggcgg agggtgagcg
    11521 gcaaaccgag catcgctgcc cggtcggtaa ggagagtggc atcggcacaa acaaccagtt
    11581 cgaccggcca ctcacgctgt gcaagctgga caattaagtc cgggccaatc ccggcgggct
    11641 cgccgggagt gatcacaaca cgttgggttt taaccattag ttgctcagga ttttaacgta
    11701 ggcgctggca cgttgttcct gcatccagct tgctgcttct tccgagaact tacggttcat
    11761 cagcatgcgg tatgcacgat ctttctgtgc cgcgtcggtt ttatcgacat tacgggtatc
    11821 cagcagttcg attaaatgcc agccgaatga agagtgaacc ggtgcactca tttgaccttt
    11881 gttcaggcgg gtcagggcgt cacggaaggc cggatcgaaa atatctggtg tagcccagcc
    11941 gagatcaccg ccctggttag cagagcctgg atcctgagag aactctttcg ctgcggcagc
    12001 aaaagtcgtt ttaccactct tgatatcggc agcaatctgt tccagtttca cgcgggcctg
    12061 ttcgtcagtc atgatcggcg acggtttcag cagaatatgg cgagcatgaa cttcggtcac
    12121 cgagatattt ttgctttcgc cgcgcaggtc gttaactttc agaatatgga agccaacgcc
    12181 ggaacgaatc gggccaacaa tgtcgccttt cttcgcggtg cttaatgcct gggcgaagat
    12241 ccccggcaac tcctgaatac ggccccagcc catctgtccg ccgttcagcg cctgctggtc
    12301 ggcagaatga gcaatcgcca gcttaccgaa atcagcgccg ttacgcgcct gatcgacaat
    12361 cgcgcgcgcc tggctttccg cttcgttcac ctgatcagag gtcgggtttt ccggcagtgg
    12421 gatcaggatg tggctcaggt tcagctcagt gctggcgtcg ttttggttac ccacttgctg
    12481 cgccagggat tcgacttcct gcggcaggat ggtgatgcga cgacgcacct cgttgttacg
    12541 cacttcagag ataatcatct ctttgcggat ctggttacga taggtgttgt agttcagacc
    12601 atcgtaagcc agacggctgc gcatctgatc cagcgtcatg ttgttctgtt tcgcgatgtt
    12661 agcaatcgcc tgatccaact gctcatcgga gattttcact cccattttct gccccatctg
    12721 caggatgatt tgatccatga tcaaacgttc catgatttgg tggcgcagcg tcgcgtcatc
    12781 aggaagttgc tgccttgcct gagcagcgtt cagttttacc gactgcatta atccatcaac
    12841 gtcgctttcc agcacgacgc cgttattgac gacggctgcg actttatcga ctacctgggg
    12901 ggcagcgaaa ctggtattcg cgatcatggc gataccgaga agcagcgttt tccagttctt
    12961 catacttttt ccatttcaat taaccgcact gcggattacg tggtaaatca acaaatcaca
    13021 aagtgttttg atacggcaga atgttcgaac gcagcatctc ttgcgtaccc agaccgtagt
    13081 tggagctcag gccgcgaagt tcgatgttaa agccgattgc gttgtcatat accgcatgtt
    13141 gtttatcgtt atcccaaccg ttcagcttcc gctcgtaacc gacgcgaatc gcatagcagc
    13201 aggagctgta ttgcacacct aacatagagt cggcttgctt gttcgcattg gtgtcgtagt
    13261 agtaggcccc aacaatggac caacgatcgg caattggcca gctggcgacg gcacctacct
    13321 gcgaaatacc attcttatat tgctcagcag tggaatagta cttaggcagc gttgcctgaa
    13381 tatattccgg gctggcgtaa cggtaattca gctgtaccag acggtcttca tcccgacggt
    13441 attcaatgct ggagttactg gtcgctatgt tatccagacg tgtatcgtac tgaatcccgc
    13501 cacgcaatcc ccaacgatca gagatgcgcc agtaagtatc gcctgcccat accagtgagc
    13561 ccgttttgtc gtcattctcc catgttatgt tgtcatcgcc agtgcgagac tccgtgaaat
    13621 agtagatttg accaacggaa atattaaaac gttcaacggc agcatcatca tatatgcgag
    13681 atgtgacacc ggtcgtcacc tggttagcgg aggcaatacg gtcaagaccg ccgtaagtcc
    13741 ggtcccggaa caggccggag tagtcagatt gcagcagcga cgagtcgtag ttatagattt
    13801 tgctctgatc gcgatatggc acatacagat actgcgcgcg tggttccagt gtctgggtat
    13861 agcccggcgc cagcatttcc atatcacgct cgaagaccat tctaccgtct accttgaact
    13921 gcggcattac acggttagcg gactctgcca gcctggtcgt gtttctggag ttataccagt
    13981 caagattggt ttgctgataa tgggttgcca gcaacttcgc ttcagtattg atgctgcccc
    14041 agttattaga gagcggcaga ttgatggtcg gttccaggtg aacacgagtc gcctctggca
    14101 tgtcatctct ggtgttaaca aagtgcaccg cctggccgta aattcgcgta tcgaaaggac
    14161 cgacatcgtt ttgatagtag ttaacgtcta actgcggctc tgccgagtag ctactggtgt
    14221 tctgctcgct gaagacctgg aactgcttgg ttgaaacggt ggcattgaag ttttgcaccg
    14281 catagccaac gctgaatttt tgcgttgcgt agccgtcggt actggaaccg tatttgttat
    14341 cgaagtcatt gaagtagcta ggatcgctga ccttggtgta gtcgacgttg aaacgccaca
    14401 cctgatccat gaccccggag tggttccagt agaacaacca acgacgtgaa ctgtcatcgt
    14461 tcgggtgttc atctttatag actttatctg aaggcagata gtccagttcc atcaagccag
    14521 cgcccgcctg ggagaggtag cggaattcgt tctcccacat gatgttgcca cgacgatgca
    14581 tataatgcgg cgtgatggtg gcatccatat ttggcgcgat gttccagtaa tatggcaggt
    14641 agaactcaaa gtagttggtg gtggtgtact tggcgttcgg gatcaagaaa ccagagcgac
    14701 gtttgtcacc caccggcaac tgcaaatacg ggctataaaa gatcggtacc ggacccacct
    14761 taaagcgggc gttccagatc tccgcaactt gttcttcgcg gtcatgaata atttcgctac
    14821 ctaccacgct ccaggtgtca gaacccggca gacaggaggt aaagctaccg ttatccagaa
    14881 tggtatagcg gttttcgcca cgttgtttca tcaggtccgc tttaccgcga ccctggcgac
    14941 ctaccatctg gtaatcacct tcccagacgt tggtatcttt ggtgttcaga ttcgcccagc
    15001 ctttcggccc tttgaggatc acctggttat cgtcgtaatg gacattaccg agcgcatcaa
    15061 cggtacgcac cggctccggt tgtcctggtg cctctttttg atggagctgc acttcgtcgg
    15121 cctgcagacg gctgttaccc tgcatgatat ccacgctgcc agtaaacacg gcgtcatccg
    15181 ggtagtcccc tttcgcgtgg tcagcattga tagtcacggg taagtcattg gtatcgccct
    15241 gtaccagagg acggtcatag cttggcacgc ccaacatgca ctgtgaggcg aggtcggctg
    15301 ccagtccctg ttgactataa agggcggtgg caatcatggt ggccaggaga gtggggatac
    15361 gttttttcat acgttgattt tattgttcca tcatcggtaa cgttgcgcgt gacaaacggt
    15421 cagagactaa cgtactcgtc atctctacgc tagtgttaat cctgtccgaa tagcgtctgt
    15481 ggtgttaggc acggcattga atgacaggta tgataatgca aattataggc gatgtctcac
    15541 aatagaccgc agccagaaat gggtaaaagc gcctttatat tgtgggagat agccctgata
    15601 tccgtgtgtc gatttgggga atatatgcag tattggggaa aaatcattgg cgtgaccgtg
    15661 gccttaataa tgggcggcgg cttttggggc gtagtattgg gcctgttaat tggccatatg
    15721 tttgataaag cccgtagccg taaaatggcg tggttcgcca accagcgtga gcgtcaggcg
    15781 ctgttttttg ccaccacttt tgaagtgatg gggcatttaa ccaaatccaa aggtcgcgtc
    15841 acggaagctg atattcatat cgccagccag ttgatggacc gaatgaacct tcatggcgct
    15901 tcccgtactg cggcgcaaaa tgcgttccgg gtgggaaaat cagacaatta cccgctgcgc
    15961 gaaaagatgc gccagtttcg cagtgtctgc tttggtcgtt ttgacttaat tcgtatgttt
    16021 ctggagatcc agattcaggc ggcgtttgct gatggttcac tgcacccgaa tgaacgggcg
    16081 gtgctgtatg tcattgctga agaattaggg atctcccgcg ctcagtttga ccagtttttg
    16141 cgcatgatgc agggcggtgc acagtttggt ggcggttatc agcagcaatc tggcggtggt
    16201 aactggcagc aagcgcagcg tggcccgacg ctggaagatg cctgtaatgt gctgggcgtg
    16261 aagccgacgg atgatgcgac caccatcaaa cgtgcctacc gtaagctgat gagtgaacac
    16321 catcccgata agttggtggc gaaaggtttg ccgccagaga tgatggagat ggcgaagcag
    16381 aaagcgcagg aaattcagca ggcctatgag ctgataaagc agcagaaagg gtttaaataa
    16441 gtttcaacgt gtaggcctga taagacgcgg cccgcgtcgc atcaggcaat cggcgcaaaa
    16501 ttgccggatg cggcgtgaac gccttctccg gcctaccgaa cttcgccagc gtgactatta
    16561 aaaatccgct ggtgctttaa acgtcatact attgccatac gccggatggg taatcgtcag
    16621 catctctgca tgtagcaaca aacgtggtgc cattgctctc gcttctggtg atgcataaaa
    16681 acgatcgccg agaatcggat gacccagcgc cagcatatgc acacgcagct gatgcgaacg
    16741 cccggtaatc ggttttaaca ccactcttgc cgtgttatcc gccgcatatt ccaccacttc
    16801 atactccgtc tgcgcaggtt tacctgtttc gtaacagact ttctgtttcg ggcggtttgg
    16861 ccagtcgcaa atcagcggca gatccaccag accttccgca ggggatggat gcccccagac
    16921 gcgggccaca tactgttttt tcggctcgcg ctcgcggaac tggcgtttta actcccgctc
    16981 cgcggctttg gtcagcgcca ctacaatcac gccgctggta gccatatcca gacgatgcac
    17041 cgattctgcc tgcggataat cacgctgaat gcgcgtcatc acgctgtctt tgtgctcttc
    17101 cagacgaccc ggcacagaca acaaaccgct cggcttgttg accaccataa tatggtcatc
    17161 ctgatacagg ataaccaacc agggttcctg cggtggattg tagttttcca tccccatctt
    17221 cggctccgtt actgatgcgt tacaacgatc aaacgcaggg catccagacg ccagcctgcc
    17281 tgatccaggc tttccattac ctgctgacgg ttgctctcaa tggcggtcag ttcgtcgtca
    17341 cgaatgttcg ggttcactgc acgcagagct tccagacgag acagctcggc agacagtttt
    17401 tcgtcggctt cgttacgcgc tgcatcaatc aatgcacggg cagatttctc gatctgcgct
    17461 tcccccagct gaaggatagc gtgaacatcc tgctgcaccg cgttaaccag cttgctgctg
    17521 gtgtgacggt taaccgcgtt aagctggcgg ttaaaggttt caaactctac ctgcgccgcc
    17581 aggttgttgc cgtttttatc cagcagcata cgtaccggcg tcggtggcag gaagcggttg
    17641 agctgcaact gcttcggagc ctgggcttca accacataaa tcagttccac caacagcgta
    17701 cctaccggca acgctttgtt tttcaacagt gaaatcgtgc tgctaccggt atcgccagaa
    17761 aggatcagat ccagaccgtt gcggatcagc ggatgctccc aggtaataaa ctgtgcatct
    17821 tcacgcgcca gcgccacttc acggtcgaag gtgatggtga tgccatcttc cgacaggcca
    17881 gggaagtccg gcaccagcat atgatcggac ggcgtcagca cgatcatgtt gtcgccgcga
    17941 tcgtcctgat tgataccgat aatatcgaac aggttcatgg cgaaggcgat caggttggta
    18001 tcgtcatcct gctcttcaat gctttctgcc agtgcctggg ctttttcgcc accgttggaa
    18061 tggatttcca gcaggcggtc acgaccttgt tccagctgtg ctttcagcgc ttcatgttgc
    18121 tcgcggcagt ttttgatcag atcgtcaaag ccttcggtct catccggcga ggccagatag
    18181 ttaatcaaat cgttgtacac gctatcgtag atagtgcgtc cggtcgggca ggtgtgctcg
    18241 aatgcatcca gaccttcgtg ataccagcgc accagcaccg actgagccgt tttttccaga
    18301 taaggcacat ggatctgaat atcgtgcgcc tgaccgatac gatccagacg accaatacgc
    18361 tgctccagca gatccgggtt gaatggcagg tcaaacatca ccatgtggct ggcgaactgg
    18421 aagttacgtc cttcagaacc gatttccgag cacagcagta cctgtgcgcc ggtgtcttct
    18481 tcggcaaacc aggcggcagc gcggtcacgt tcgataatcg acatgccttc gtggaacact
    18541 gcggcgcgaa taccttcacg ttcgcgcagt acctgctcca gttgcagcgc agtggccgct
    18601 ttggcgcaga tcaccagcac tttctgagag cgatggctgg tcaggtagcc catcagccat
    18661 tcaacgcgcg gatcgaagtt ccaccaggtg gcgttatcgc cttcaaattc ctgataaata
    18721 cgctccgggt agagcatatc gcgagcgcga tcttccgcac ttttacgtgc gcccataatg
    18781 ccggagactt taatagcggt ctgatactgc gtcggcagtg gcagcttaat agtatgcagc
    18841 tcgcgtttcg ggaagccttt cacaccgtta cgggtgttac ggaacagcac gcggctggtg
    18901 ccgtggcgat ccatcagcat cgaaaccagt tcctgacggg cgctctgggc atcttcgctg
    18961 tcgctgtttg ctgcttgcaa cagcggctcg atatcctgct cgccgatcat ctcaccgagc
    19021 atgttcagtt cgtcattgct gagtttgtta cctgccagca gcatggcaac ggcgtccgca
    19081 accggacgat aatttttctg ctcttcaacg aattgcgcaa agtcgtggaa acggttcggg
    19141 tccagcagac gcagacgggc gaagtggctt tccatcccca gctgttccgg ggttgcggtc
    19201 agcagcagaa cgcccggcac gtgctctgcc agttgttcaa tggcctgata ttcacggctc
    19261 ggcgcatctt cgctccacac caggtgatgc gcttcatcga ccaccagtag atcccactcg
    19321 gcttcacaga gatgttccag acgctgtttg ctacgacggg caaaatccag cgagcaaatc
    19381 accagctgct cggtatcgaa cgggttgtaa gcatcgtgct gagcttcggc ataacgctca
    19441 tcatcaaaca gagcaaagcg caggttgaaa cggcgcagca tttctaccag ccactgatgc
    19501 tgtaaggttt ccgggacgat aatcagcaca cgttcagcag cgccagagag cagttgctga
    19561 tgcaggatca tcccggcttc aatggttttc cctaaaccca cttcgtcagc caacaggacg
    19621 cgcggcgcgt ggcggcgacc aacatcatga gcgatgttga gctggtgcgg gatcaggctg
    19681 gtacgctgac cgcgcaggcc gctgtacggc atacggaact gttcgctgga atacttacgc
    19741 gcgcgataac gcagcgcaaa gcggtccata cggtcaatct gcccggcaaa cagacggtcc
    19801 tgcggtttgc tgaacaccag tttgctatca aggaaaactt cacgcagggc cacgccggac
    19861 tcttcagtat caaggcgagt accgatatag gtcagcaagc cattttcttc ttttacttct
    19921 tcgacttgca tctgccagcc gtcatggctg gtaatggtat caccagggtt gaacatcacg
    19981 cgggtcacgg gggaatcact gcgtgcgtac agacggtttt caccagtaga tgggaaaagt
    20041 aaagtgacag ttcgcgcatc catcgcgaca acggttccaa gtcccaattc gctttctgta
    20101 tcgctgatcc agcgttgacc aagtgtaaaa ggcatatgtg ttcggctcta tatctttaat
    20161 tgcaggcaat aaccacccgc taccgtgctt atgaggtagt ggtgttattc aggtccagga
    20221 atggaaaggg cgctatggta ctggatggca aagcattcgt cacgcatcaa aatggtatct
    20281 ggcgaactct tttttttgct caaaatagcc caagttgccc ggtcataagt gtagcaaaat
    20341 tatcctcaat aaaagggagt attccctccg ccacgggttg tagctggcgg gtcagatagt
    20401 gttcgtaatc cagtggtgaa cgttggtagt ccagcggctc cgggccgttg gtggtccata
    20461 cgtacttaat ggtgccgcgg ttctgatatt gcaaggggcg accgcgctta tggttttctt
    20521 catcggcaag gcgagcggcg cgtacatgag gcggaacatt acgctgatat tcgctcagcg
    20581 gacggcgaag gcgtttacgg taaaccagct gcgtgtccag ttcgcccgcc atcagcttgt
    20641 cgatggtttc gcgcacgtat tcctgatatg gctcgttgcg gaagatacgc aggtacagct
    20701 cctgctgaaa ctgctgggcc agcggcgtcc agtcggtgcg cacggtttcc agccctttaa
    20761 ataccatccg ctgtttgtcg ccctcctgaa tcagtccggc gtagcgcttt ttgctgccgg
    20821 tatcggcccc gcgaatggtt ggcatcagaa aacggcagaa atgggtttca tactccagtt
    20881 ccagtgcgct ggtcagccgt tgtttttgca gcgtttccgc ccaccaggcg ttaacgtgct
    20941 gcaccagtgc acgaccgatt ttcgctgctt cttcttccga atgtgcacct ttcagccaga
    21001 caaacgttga gtcggtatcg ccgtagataa catcatagcc ctgcgcttca atcaacgttt
    21061 tggtttgccg catgatctga tgaccacgca tggtgatcga cgatgccagg cgtggatcga
    21121 agaaacggca ggcggtggtg ccgagcacac cataaaaggc attcataatg atcttcagcg
    21181 cctgcgaaag cggcttatta ccctgacgtt tggcttcatc gcgcccgtgc cagatgttag
    21241 tcacaatctc cggcaggcaa tgtttctccc gcgagaacca ggcatcgaga aaaccttcgg
    21301 tactgtgctc tggatcaggc tgcgccatgc cttccaccag cccgacggga tcaatcagaa
    21361 aggtgcggat gatcgacggg tacaggcttt tgtagtccag caccagcacc gaatcataaa
    21421 gcccaggtcg cgaatccatc acgtagccgc cagggctggc gtgtggcggc acttcgccga
    21481 gattaggcgc gacataacca gcgcgatgca tacggggaaa atagagatga ccaaacgctg
    21541 ccaccgaacc gccgtgtcga tctaccggca ggccgttcac cgtcgcccgt tcgagcaaaa
    21601 acggcatgat ttcagttttg tggaagatct gcgtcaccag ctcgcaatct ttcaggttat
    21661 aaattgccag cgcaggctta tcttcggcga aacggcggtc aatttcgtcc attcgatccc
    21721 acgggttatc gatagatttt ccttcgccta ataactcctg agcgacggtt tccagcgaga
    21781 atgaagagaa attccagaac gcggatttca gcgcctcgat accgtcgata atcagtcgcc
    21841 ctttggcctg agcaaaaaag acgccgtttt taaagccgtg ctcgcgccac tccagctcgc
    21901 tgttatcacg tcccagacgc agcggaatac ggtaacgctc ggcatgtttt tgcagcattc
    21961 gcagatcgaa ctgcaccacg ttccaaccga tgatcacatc aggatcatga gtcgcaaacc
    22021 aggcgttaag tttttccagt agcagcgggc ggctggcgac gtattccagt tcaaaatcga
    22081 gcgtggaggc gtcgccattc tctggcccca gcatataaac gatacgctgc ccgcaacctt
    22141 ccaggccgat gcagtacagc tcaccgtggc gggtggtttc aatatcaata gaaacccact
    22201 tgagcggcgg acgatagtcg ggatgcggtt tcagacgggc attaacgata gcgccattgc
    22261 gtatatcacc ctcgacccac accggtgagg tgataaaccg ctccatcaga tagcgttctg
    22321 gcgggcgcac atcggcctcg tagacggtga cgccaccttc acgcaggcgc ttttcgtaat
    22381 tcatcaactg gcgatgggcg cgacagtaaa ggccatatac cggctggcgg tgaaaatctt
    22441 ttaacgccag cggcgtcagg cgaaagcctt gttcaccctg caaaatatgc tgagcgcggg
    22501 gaacctgatc ggcgggaata aatgccacgg actcttgcgg cgcaagcgta acctgcaacg
    22561 gcccgttgtc cgttgccagc cagaaggaga cctctgttcc ttgcggggtg tcccgccagt
    22621 gtcgggttaa gataaaacct gcctgcgcca cgctgaaaat ccaataaaaa accaggcttg
    22681 attatagcct ggtttcgttt gattggctgt ggttttatac agtcattact gcccgtaata
    22741 tgccttcgcg ccatgcttac gcagatagtg tttatccagc agcgtttgct gcatatccgg
    22801 taactgcggc gctaactgac ggcagaatat ccccatataa gcgacttctt ccagcacgat
    22861 ggcgttatgc accgcatctt cggcgttttt tccccatgca aatgggccgt gagaatggac
    22921 cagcacgccg ggcatttgcg ctgcatcgat accctgtttt tcgaaggttt ctacgatgac
    22981 gttaccggtt tcccactcat attcaccgtt gatttctgcg tcggtcattt tgcgggtgca
    23041 gggaatggga ccgtagaaat agtcggcgtg ggtggtaccg gttgccggaa ttgactggcc
    23101 cgcctgcgcc cagatggtgg cgtggcgcga gtgtgtgtgc acaatgccgc caatagacgg
    23161 gaatgcctga tagagcagtc ggtgagttgg cgtgtcggag gagggctttt tcgtaccttc
    23221 aaccacttca ccggtttcga tgctgaccac gaccatatcg tcagcggtca tgatgctgta
    23281 gtcgacgccg gaaggtttga tcaccaggac gccgcgcccg cgatcaacgg cgctgacatt
    23341 gccccaggtg agcgtgacca ggttgtgttt tggcagcgcc aggttggctt ctaatacctg
    23401 gcgtttgaga tcttctaaca tgttgactcc ttcgtgccgg atgcgctttg cttatccggc
    23461 ctacaaaatc gcagcgtgta ggcctgataa gacgcgtcgg cgtcgcatca ggcggtgaat
    23521 gccggatgcg ctttgcttat ccggcctaca aatccggaac gcgtaggcct gataagacgc
    23581 gccagcgccg catcaggcgg tgaatgccgg atgcgctttg cttatccggc ctacaaatcc
    23641 ggaacgcgta ggcctgataa gacgcgtcag cgtcgcatca ggcgttacat acctgatgcg
    23701 gctacttagc gacgaaatcc gtaatacact tcgttccagc gcagcgcgtc tttaaacgct
    23761 ggaaggcggg tatcgttatc aatcactgtg atttcaatgt cgtgcatctc ggcgaactgg
    23821 cgcatatcgt tgaggttcag cgcatggctg aagacggtat ggtgcgcgcc accagcaagg
    23881 atccacgctt cggaagcggt tggtagatcc ggttgcgctt tccacagcgc attcgccacc
    23941 ggcagtttcg gcagggagtg cggtgttttc accgtgtcga tgcagttaac cagcagacgg
    24001 taacgatcgc cgagatcaat caggctggcg acaatggctg gaccggtttg agtgttgaag
    24061 atcaggcggg caggatcgtc tttaccgcca ataccgagat gctgaacgtc gaggatcggt
    24121 ttctcttcca cagcgatcga cggacagact tccagcatat gggagccgag taccaggtca
    24181 ttacctttct cgaagtgata ggtgtagtcc tccataaagg aggtgccgcc ctgcagaccg
    24241 gttgacatca ccttcatgat gcgaagcagg gcggcagttt tccagtcgcc ttcgcccgca
    24301 aagccgtagc cctgctgcat cagacgctgt acggccagac ccggaagctg tttcaggccg
    24361 tgcaaatctt caaaggtagt agtgaaagcg tggaagccac cttgttccag gaaacgcttc
    24421 atccccagct caatacgcgc cgcttccagc acgttctgtc gtttttcgcc gtggatttgt
    24481 gtggcaggtg tcatggtgta gcagctttcg tactcatcga ccagcgcgtt aacatcgccg
    24541 tcgctgatgg agttcaccac ctgcaccaga tcgccaaccg cccaggtatt gacggagaaa
    24601 ccaaacttga tctgtgcggc aactttatcg ccatcggtga ccgctacttc acgcatgtta
    24661 tcgccaaaac ggcagacttt cagatgacgg gtatcctgtt tagagaccgc ctgacgcatc
    24721 caggagccaa tacgctcatg tgcttgttta tcctgccagt gaccggtaac aacggcatgt
    24781 tgctgacgca tacgcgcgcc gatgaagccg aactcgcgac cgccatgcgc ggtctggttc
    24841 aggttcataa agtccatgtc gatgctgtcc cacggcagcg ccgcgttgaa ctgggtgtgg
    24901 aattgcagca acggtttgtt gagcatggtc aggccgttga tccacatttt ggccggggag
    24961 aaagtgtgca gccacactac cagcccggcg caacgatcgt cgtaattcgc gtcgcggcaa
    25021 atagcggtga tttcatccgg cgtggtgccc agcggtttca gcaccagttt gcagggcaac
    25081 ttcgcttctg tattcagtgc attaacaacg tgttccgcat gttgcgtcac ctggcgcaga
    25141 gtctccgggc cgtaaagatg ttggctgcca attacaaacc acacttcata attatcaaaa
    25201 atcgtcattg tcgtgtcctt atagagtcgg aacggcctgg gcagcctgtg ccggggcgga
    25261 agttggaaga tagtgttgtt cggcgctcat cgcccattgc tgatagcggc gataaagctg
    25321 ttcaaagcgt tgtgcctgtt cgctgcgcgg ttgcagggtt ttctctaccg cactggccat
    25381 tttttgctgg gctgatggga tgtctgcgtg cactttcgcg gcgacagcag caaaaatcgc
    25441 cgcaccgagc gcacagcact ggtcagaggc aacaatttgc agcgggcgat tcagcacgtc
    25501 gcagcaggcc tgcataatga cctggttttt ccgcgcgatg ccgcccagcg ccatcacgtt
    25561 attgacggcg atcccctgat cggtgaagca ctccataatt gcgcgtgcgc caaaggcggt
    25621 ggcggcaatt aaaccgccga acagcagcgg agcgtcggtg gcgaggttca gatcggtaat
    25681 cactcctttc aggcgttggt tagcgtttgg cgtgcggcga ccgttaaacc agtcgagcac
    25741 caccggcagg tgatccagag acggattttt ggcccatgct tcggtcagcg ccggaagcag
    25801 ttgtttctga ctggcgttga tttgctcttt cagttccgga tgctgggcgg caagctgttc
    25861 cagcggccag ctgagtacgc gtccaaacca ggcgtagata tccccaaacg ccgattggcc
    25921 tgcttccaga ccgataaatc caggcaccac gctgccatca acctgaccgc aaataccttt
    25981 cactgcccgc tcgccaacgt tctgtttgtc ggcaatcaga atgtcacagg tggaagtacc
    26041 gataactttt accagtgcgt taggctgtgc gcctgcgcca actgcgccca tatggcagtc
    26101 aaacgcgccg ccggaaataa ctatgctttc aggcaggccg agacgctgcg cccattccgg
    26161 gcataaggtg cccaccggaa tatcggcagt ccaggtatca gtgaacagcg gggaaggcaa
    26221 atggcgattg aggattgggt ccagctcatc aaagaaactg gctggcggca ggccgcccca
    26281 gctttcgtgc cacagcgatt tatgtccggc gctgcaacgt ccgcgacgaa tatcctgcgg
    26341 gcgcgtggtg ccactgagca gggcgggcac ccagtcgcac agctcaatcc atgatgcggc
    26401 agattgtgcc acggcgttgt cctggcgagt tacatgcagg atttttgccc agaaccattc
    26461 gctggaataa ataccgccaa tatagcggga gtagtcaacg ttgcccggcg tgtgacataa
    26521 gcgggtaatc tcttccgctt cttcaaccgc ggtgtggtct ttccacaata cgaacatcgc
    26581 gttcgggttt tcggcaaact ccgggcgcag cgccagcacg ttaccgtcgg cgtcaatcgg
    26641 tgcgggcgtc gagccggttg tgtcaacgcc aatcccgacc acagctgcgc gctgttcgac
    26701 gctaagctct gcaagcacag ttttcagcgc cgcttccatt gactcaatgt agtcacgcgg
    26761 atgatgacgg aactggttat tcggggcatc acaaaattgc ccgttctgcc aacggggata
    26821 ccactctacg ctggtggcga tctcttcacc ggtggcacat tccaccgcca aagctcgcac
    26881 agaatcactg ccaaaatcga ggccaattgc aatcgccatc gtttcactcc atccaaaaaa
    26941 acgggtatgg agaaacagta gagagttgcg aaaaaaaacg tcaggctgga tccgctaatc
    27001 ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa tcaatgtgga
    27061 cttttctgcc gtgattatag acacttttgt tacgcgtttt tgtcatggct ttggtcccgc
    27121 tttgttacag aatgctttta ataagcgggg ttaccggttg ggttagcgag aagagccagt
    27181 aaaagacgca gtgacggcaa tgtctgatgc aatatggaca attggtttct tctctgaatg
    27241 gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt
    27301 ttaacgccca tctggtggcg ggtttaacgc cgattgaggc caacggttat ctcgattttt
    27361 ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt cgcggtcagg
    27421 gggtggtgaa aaatcaggga cgagaatttg tctgccgacc gggtgatatt ttgctgttcc
    27481 cgcctggaga gattcatcac tacggtcgtc atccggaggc tcacgaatgg tatcaccagt
    27541 gggtttactt tcgtccgcgc gcctactggc atgaatggct taactggccg tcgatatttg
    27601 ccaataccgg tttctttcgc ccggatgaag cgcaccagcc gcatttcagc gacctgtttg
    27661 ggcaaatcat taatgccgga caaggggaag ggcgctattc ggaattgctg gcgataaatc
    27721 tgcttgagca attgttactg cggcgcatgg aagcgattaa cgagtcgctc catccaccga
    27781 tggataatcg ggtacgcgag gcttgtcagt acatcagcga tcacctggca gacagtaatt
    27841 ttgatatcgc cagcgtcgca cagcatgtct gcctgtcgcc gtcgcgtctg tcgcatttgt
    27901 tccgccagca gttagggatt agcgtattaa gctggcgcga ggaccagcgc atcagtcagg
    27961 cgaagctgct tttgagcacc acccggatgc ctatcgccac cgtcggtcgc aatgtcggtt
    28021 ttgatgatca actctatttc tcgcgagtat ttaaaaaatg caccggggcg agtccgagcg
    28081 agtttagggc aggttgtgaa taataagaaa gaaggggggg aaccccct
//
LOCUS       DAZKHK010000066        28088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKHK010000066 DAZKHK010000000
VERSION     DAZKHK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.066"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHOS5_002806"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHOS5_002806"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9777597.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQCYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHOS5_002807"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHOS5_002807"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP9777598.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACHOS5_002808"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACHOS5_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9777599.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACHOS5_002809"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACHOS5_002809"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP9777600.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACHOS5_002810"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACHOS5_002810"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP9777601.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACHOS5_002811"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACHOS5_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP9777602.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /locus_tag="ACHOS5_002812"
     CDS             complement(8377..9201)
                     /locus_tag="ACHOS5_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP9777603.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACHOS5_002813"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACHOS5_002813"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9777604.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGREADLRTLVDSAHQRGIRILFDIVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYISSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGVGKQTTLSLKQGYGFVRDHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACHOS5_002814"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACHOS5_002814"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP9777605.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13134..13607)
                     /locus_tag="ACHOS5_002815"
     CDS             complement(13134..13607)
                     /locus_tag="ACHOS5_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9777606.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="ACHOS5_002816"
     CDS             complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="ACHOS5_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP9777607.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14758..15756
                     /gene="yiaK"
                     /locus_tag="ACHOS5_002817"
     CDS             14758..15756
                     /gene="yiaK"
                     /locus_tag="ACHOS5_002817"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP9777608.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15768..16232
                     /locus_tag="ACHOS5_002818"
     CDS             15768..16232
                     /locus_tag="ACHOS5_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP9777609.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            16255..17175
                     /locus_tag="ACHOS5_002819"
     CDS             16255..17175
                     /locus_tag="ACHOS5_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HFP9777610.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINSLQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
     gene            17289..17756
                     /gene="yiaM"
                     /locus_tag="ACHOS5_002820"
     CDS             17289..17756
                     /gene="yiaM"
                     /locus_tag="ACHOS5_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP9777611.1"
                     /translation="MKRILEGILAIIIAILSCIIFINIILRYGFHTSILSIDELSRLL
                     FVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEK
                     TAQDWSNLSPILGVPIGLMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            17759..19036
                     /gene="yiaN"
                     /locus_tag="ACHOS5_002821"
     CDS             17759..19036
                     /gene="yiaN"
                     /locus_tag="ACHOS5_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP9777612.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            19049..20035
                     /gene="yiaO"
                     /locus_tag="ACHOS5_002822"
     CDS             19049..20035
                     /gene="yiaO"
                     /locus_tag="ACHOS5_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP9777613.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            20039..21535
                     /gene="lyxK"
                     /locus_tag="ACHOS5_002823"
     CDS             20039..21535
                     /gene="lyxK"
                     /locus_tag="ACHOS5_002823"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP9777614.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGIPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGIYHNFSE
                     AQRDLQHPVRTLLPNMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            21532..22194
                     /gene="ulaD"
                     /locus_tag="ACHOS5_002824"
     CDS             21532..22194
                     /gene="ulaD"
                     /locus_tag="ACHOS5_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9777615.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTRLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRSLAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            22187..23047
                     /locus_tag="ACHOS5_002825"
     CDS             22187..23047
                     /locus_tag="ACHOS5_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9777616.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTK
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            23041..23736
                     /gene="araD"
                     /locus_tag="ACHOS5_002826"
     CDS             23041..23736
                     /gene="araD"
                     /locus_tag="ACHOS5_002826"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9777617.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AKIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(23783..25321)
                     /gene="aldB"
                     /locus_tag="ACHOS5_002827"
     CDS             complement(23783..25321)
                     /gene="aldB"
                     /locus_tag="ACHOS5_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP9777618.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(25429..26724)
                     /locus_tag="ACHOS5_002828"
     CDS             complement(25429..26724)
                     /locus_tag="ACHOS5_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFP9777619.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(26854..28005)
                     /gene="yiaY"
                     /locus_tag="ACHOS5_002829"
     CDS             complement(26854..28005)
                     /gene="yiaY"
                     /locus_tag="ACHOS5_002829"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP9777620.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         6932 a         7233 c         7052 g         6871 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttctcgt cgtggctgat aagcggcata acactgcgca tctggtagat
      121 gcgactgttc taacggtagt tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcgccaccc gtgcggtaat
      241 ccagttgctg accgctgatg tccgccagca tttgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccctttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcgg
      541 tgattaaagc agggacattg ctcaggccgg ttaatttcgc ggcccaatcc agacacgaag
      601 ccgcactcag cattacagac attaaatgcc aacgttgcgg taacgcatgg caaaagctat
      661 gtacggcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaacc gcaccagctg
      781 cattgtcgcc accgcctgcg ataactggca ccgccgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcgtataa tgcgggcatc tggtcacgag
      901 ataagtggca agcctgcagc atgagatcgc tccagtcacg ctttgcgaca tccagccaca
      961 tagtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttatcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcacccgcg
     1141 attgcggaac tcgcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgtaacacc cgttgctgag catccagtaa ggttgcaccg tgcatctgtc
     1261 cggcaatacc taatgcttta acgtcttgca gagaatgctg aacgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgcag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac tgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcctaatt cactattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccaacgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcta tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaata ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcagca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtgccc tggaaaccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagctgttca cgctcctggc gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc accccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccaactga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcagttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catatccgcc ccgttccagc agaaggtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatggg atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aagcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgattaacc
     3481 ggggtgtcga tgttcttgtc attattcctt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga ccgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataatg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc gggcaaatga aagtattaaa accttatgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggaggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatcttgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacattgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggag ccaaaagcgg ataccacact gaataatggc ctgaaagatg
     4141 ttccctcccg cctgctgaca ccgatcgatg tgaataaaaa caacatcaaa gatacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcagg
     4261 ggcgtgattt ctctccatgc cgcgttaatt aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaaaaata ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 cagcttgcgg ttgaatgctg gcgaaatcgt ctcactctgt ggggaaaatg ggtcaggtaa
     4441 atcaacgctg atgaaagtgc tgtgtggtat ttatccccat ggttcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccattatt caccaggaat tggcgctggt gaaagaactg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc ataatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 aggacttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgttt
     4801 gttaattctc gatgagccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggtcaac acattggtac
     4981 gcgtgatgct gctggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg ttaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtatcgc cggactcgtt ggtgccggac gtaccgagac
     5221 tattcagtgc ctgtttggtg tgtggcccgg acaatgggaa gggaaaattt atattgacgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aaaaaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaatcc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcaacaaaa agcgatcctc gctcgctgtc tattacttaa
     5581 cccgcgcatt ctcattcttg atgaacccac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgtgtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attagccgta ccgacatccg
     5881 gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccaa accgcaatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggctg ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggctaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggagcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg tggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag caccggcttc atcattggcg cgcttggctt aatggctttt gttggctggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg tgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cggcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gctattaacg
     6721 gattaatggt agccatcgcc ggattaatcc tcagttctcg actcggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg tgtgattggc ggcaccagtc
     6841 tggcaggcgg cgtcgggagc gtggctggag cggtaatggg ggcattcatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg taacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acaaatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gtggctcgta tcaccttgcc gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggtaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagattg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgga ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattaccgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actcgatgcg gtgggcatat cacgttccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcgcatcagg taactcagca caaccacctg atgcgagcaa aagccttatc
     8341 cgactgacaa gtcggataag gctcgaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta
     8461 gcccttcaac ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 tgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttgaggttag taacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtacccc ttcactttac ccggcgcatt
     8641 agtacaacgt cctttcatgc atttcatgcc gaacagattg ttgttgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgct accatactgg tggggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatgagcc agttacgttc cgcagtaatg gccgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgga gaaacgcttt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 attttttaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgagtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcgc gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccgcc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggtttcccgc
     9601 ctttagcgaa caggggacgg gaacatttgt cagccacgca cagttgccca aaggtacgcg
     9661 tccactaacg ctaaattttg accaacagtg ctggcagcct gcggatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcaggga
     9781 cggcgaatat acgctgcaac tagacacccg ctccggtaca ccaacattga tgatttccat
     9841 ccagaacgcc gtcgaaccgg tagcaagcct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcaca ctggatgtca gcgccacttt tccggaggga gccgccgtcc gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgccaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc tctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcagaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ctgggcgtca atgctttatg
    10261 gataagtgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggccgcgaa gccgatctac ggacgctggt tgatagcgcg catcagcgcg gtattcgtat
    10441 tctctttgat atcgtgatga accacactgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgagctgaaa aaaacgctgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg
    10681 cgattacgac aatcctggat tcgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaactaccg cttctggtct gccagtgttc tataaaaaca aaacggatac
    10801 ccacgctaaa gtcatcgacg gctttacccc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgctgca cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcagag aaattgcagg atttcaacgt gttgagctac atctcgtcgc atgatacccg
    11221 cctgttccgt gaagggggcg acaaagcagc agagttatta ttattagcgc caggcgcggt
    11281 acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctaccggtt ctgatccgct
    11341 gcaaggtacg cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccagcgt
    11401 cgcgcactgg caaaaaatca gccagttccg cgcccgccat cccgcaattg gcgtgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgatcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcattgcagg ataaagcccc
    11641 gctcactctt caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattatga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggctgggat atcgaaccac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatcta catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggtattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 cgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 gctacctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ccgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 gattcataaa ccggaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcag ctctaccagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttcccg ggactggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg tgtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacatga
    13021 tttctgcaat atattgaatt tggtagaatt tgtaggccgg ataaggcgtt ttagccgcat
    13081 ccggcataaa caaagcgcac tatccctgcg ttgagaaacg ccggaaaaac gtttcaaaca
    13141 ggctgctgcc gtagccgatg tcgctgcact ttctcgacat ccatgcactg caacgccttt
    13201 gtcgggcagg cttcaacaca ggccggcccc gtcgcccgat gccagcacag atcgcactta
    13261 atcgccctcg cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc
    13321 atacagcttt tacagccaat gcaacgtgat tgttcaacga aaatatgccc atgttcgcgg
    13381 cttatcgcgt caacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct
    13441 actgccgtgg tccagctgtg gtctttaata acacgaatac gggaaataaa ctcgtctggt
    13501 gacaacgcag cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta
    13561 cggcaaccga tacatttcgt cgcatccgca ataataaacc ggttcatgat cactcctttg
    13621 cactaaaaca gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat
    13681 gttaaagttt tggcacttgt ccaaagggtt atgtaatcgc gccctggtcg tccctgacag
    13741 taaatcccag ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt
    13801 tctctcccac ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc
    13861 gcccatgaat atcaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc
    13921 tgtccatcgc cgctccactt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct
    13981 cggtaatggt attgcgggtt aacggctgga tctcatgctg atggctttcc cagtatgact
    14041 tcacgtagtc cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg
    14101 gcatatgctg gcctatatag gcacgggttc gcagcatccc ggttgtgggt tccaacttat
    14161 aaatcaaaat agcgtgatcg tcttcgcggc tggagaagtt aatagtttca ccagtggcga
    14221 tgttcagagc ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gatagcgcct
    14281 tctgcccgac ggcaataaat ttggtggtca ggcgataact ccctgcggcg ggcgcggtgg
    14341 tcacatagcc gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac
    14401 cagccagctc cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa
    14461 tcagcatcaa cccacgaaac agactctggc ttccggctgg gcgctctttt tcctgcgcca
    14521 tctcgttctc tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg
    14581 caaagtgtgc cgtagttcac gatctcgaca gataatttat aaccacttga tttttaagtt
    14641 ttttgaaatt catcaatcag attgctttgt taaaaagcga tcgatatatt tgaaatcaag
    14701 tttcgtatat tgaaatttta agccaaaaaa gcgatcaaaa aaacaaggaa gcctgggatg
    14761 aaagtgacat ttgagcagtt aaaagcagcc tttaatcggg tcttaatttc acgcggcgtt
    14821 gacagcgaaa cggctgacgc ctgtgcagag atgttcgccc gcaccaccga atcaggcgtt
    14881 tattctcacg gcgttaatcg tttccctcgt ttcattcaac aactggaaaa cggcgatatc
    14941 attcctgatg cccaacccaa acgtataacc agcctcggcg caattgaaca gtgggacgcc
    15001 cagcgttcga tcggtaacct gacagcgaaa aagatgatgg atcgcgccat tgaactggct
    15061 gccgatcacg gtattggtct ggtggcacta cgtaatgcca accactggat gcgcggcggc
    15121 agctacggct ggcaggcggc ggaaaaaggc tatattggca tttgctggac caactccatc
    15181 gccgtaatgc caccgtgggg cgcaaaagag tgtcgcatcg gcaccaaccc gctgatcgtc
    15241 gccattcctt ccacgccgat caccatggtc gatatgtcga tgtcgatgtt ctcttacggc
    15301 atgttagaag ttaatcgcct ggctggtcgt cagctcccgg tagatggtgg ctttgatgat
    15361 gagggcaatt tgaccaaaga acctggcgtt atcgagaaaa atcgccgcat tttgccgatg
    15421 ggctactgga aaggttcggg catgtcgatt gtgctggata tgatcgctac cctcctttct
    15481 gacggcgcat cggttgccga agtcaccgag gacaacagcg acgaatacgg catttcacaa
    15541 atttttattg ccattgaagt ggacaagctt atcgacggtc ccacccgcga tgccaaactg
    15601 caacgcatta tggattacgt taccagtgcc gagcgtgctg acgaaaacca ggctattcgt
    15661 ttacccggcc acgaatttac taccctgctg gcagaaaacc gccgtaacgg tattaccgtt
    15721 gatgacagcg tgtgggcaaa aatccaggcg ttataaggag ataagccatg atttttggac
    15781 atatcgcgca gcctaatccg tgccgtttgc ccgccgccat tgaaaaggcg ctcgattttc
    15841 tgcgcaccac cgacttcaac gccctggaac cgggcgttgt cgaaatcgac ggcaagaata
    15901 tttatgcgca aattatcgat ttaaccacac gcgaggcggt agaaaaccgt ccggaagtgc
    15961 atcgtcggta tatcgatatt cagtttctgg cgtggggtga agagaaaatc ggcattgcta
    16021 ttgatacggg aaataataaa gtcagcgaat cactattaga gcagcgcgat attatttttt
    16081 atcacgatag tgaacatgaa tcatttatag aaatgattcc gggcagctac gctattttct
    16141 tcccgcagga tgttcatcgc ccggcatgta ataaaaatgc cactacggca atacgcaaga
    16201 tcgtggttaa agtggccctc gctgctttat aaaaaatgat tactggagca cataatgaaa
    16261 acgaataatg ccggttatat tatcggcgcg tatccctgtg caccttcttt tcaccaaaga
    16321 agtgaagagg aagagaagga tttctggcga caattatctg agactccaga cattttgggg
    16381 ctggaacaac cttgcctgga aaattttcac cctcttggcg atcaatggtt actccgtcat
    16441 acgccagaga gctggaaatt tgtcgtcacc gcagtgatgg aaaccatgcg tcgccgcagc
    16501 gaaaacagcg ggtttggtct cgcctccagt gatgaagaac agcgccaggc ctgcgtggcg
    16561 tactatcgcc acctgttcaa taaaatcaac tcattgcagg ctaataaagt gctcgcactg
    16621 gagctccagg ctgcaccgtt agcaacgaat cctaatgtta tgcaagcgac ggacgctttt
    16681 gcccgctcat tgaaagaaat cgccagttgg gactggccgt gcaagctggt gctggaacac
    16741 tgcgacgcca tgacatcttc ttccccccga aaaggctttt tgcctttaga aaatgtgctg
    16801 gaagttatta ccgattatga catcagcatc tgtatcaact gggcgcgctc tgccattgaa
    16861 gggcgcaata caacgctgcc gctgacccac acccaaatgg cgaaacaagc cggaaaactc
    16921 ggcgcgttaa tgttctctgg tacgacgcta aacggggcat acggcgaatg gcaggattta
    16981 cacgcaccgt ttgcaccatt ctgcgcagaa agcctgatga ccacagatca tgttcgcgag
    17041 ttatttaatg tagcagaatc gtcaacattg cattttgctg ggatcaagct actggaaata
    17101 aatgcgacgg ctgatgtgca ccatcgcatc gaaatattac gcaacggcat tcactctctg
    17161 aatgaatctc gttaaacaga accatttata tatcactttg aatatctaat tagccgtttt
    17221 ctgataatta ttacgaatat taacaacatt agcgcataaa aaatattgca taaccagaag
    17281 gactcgttat gaaacggata ctcgaaggca ttcttgcgat catcatcgca atactgtctt
    17341 gtattatttt tattaatatc attcttcgat atggattcca cacaagtata ttatcgattg
    17401 atgaattatc ccgactttta tttgtctggt taacctttat tggcgcaatc gttgcttata
    17461 tggacaacag ccatgtgcag gttacttttt tagtcgaaaa actctcaccg gctaatcaac
    17521 aacgtgtttc gctgctaacc catacgctca ttttattgct gtgtctgggg ctggcctggg
    17581 gcgctataga aaaaacagcg caagactgga gcaacttatc gcccattctt ggcgtcccca
    17641 ttggcttaat gtacgccgcc gctatcccca ccagtctgat tatcgcattg ctggaattgc
    17701 gtcacttata ccggcagttc acaaataccc ccagccgaaa ccaacaagga gcctgacgat
    17761 gacaatcctt atatttattg tcagtttgtt aggggctatc gcaattggcg ttcccatcgc
    17821 ctgggcatta ttactgtgcg gcatttcatt gatgttctgg atggatatct ttgatgtgca
    17881 aattctggca caaacgttgg taaatggcgc agacagcttc tccctgttag ccatcccctt
    17941 ctttgtactg gcgggtgaga tcatgaacgc gggcggcctg tcgcaacgta tcgtcgactt
    18001 gccaatgaaa ctggtaggcc acaggccagg cggtctgggt tacgtgggtg tgctggcagc
    18061 gatgatcatg gcaagcctgt ccggttctgc ggttgctgat accgcagctg tcgcggcgct
    18121 gctggtgccg atgatgcgcc aggccaacta tccggtaaac cgcgccgccg ggcttatcgg
    18181 ttccgggggg attattgcgg ccattatccc gccttccata ccactcatta tcttcggcgt
    18241 ctccagcgga ctctccatca gcaaactgtt tatggcgggc atcgcaccgg gcatcatgat
    18301 gggcgtcacg ctgatggtta cctggtggtg gcaggcaaaa cgactgaatt taccttgtca
    18361 gccgaaagcc tcactgcgag aagtctggca atccttagtt tccggtattt gggcgctctt
    18421 tttaccgata attattattg gcggtttccg ttcggggtta tttacaccga ccgaagcagg
    18481 cgcggttgcc gccttctacg ctctgttcgt ctcggtggtg gtctatcgcg agatgacctt
    18541 ctccacgctt tatcatgtgc tgattaacgc ggcaaaaacc acatccgtgg tgatgttcct
    18601 ggtggcctct gctgcggtat ctgcatggct tattacgatc gccgaattac ccatgatggt
    18661 atcggagcta ttacaaccgt tggttgattc gcctcgctta ttattcatcg tcattatgct
    18721 cgctattatg gtgatcagta cggtcatgga cttaacgcct accgtattaa ttcttacacc
    18781 agtattaatg ccattagtga aagaagcggg aatagatccg gtctatttcg gcatcatgtt
    18841 tattatcaac tgttcaatat cactgattac gcctccagtc ggtaatgttc ttaacgtggt
    18901 atgcggcgtg gcgaaactta aatttgacga tgcggtaaaa ggcgttgcgc cttacgtcat
    18961 ggttttattc atgttgttag cattatttat ttttataccg gagctcatta ccgctcctct
    19021 taaatggatg agttaaaagg aaatcataat gaaattacgt tctgtaacct acgctttatt
    19081 aattgccgga gtggcgacgt tcagcacatc ttctctggcg gcacaatcat tacgtttcgg
    19141 ttatgaaacc tcacaaaccg attcacaaca tattgcggcg aaaaaattta atgaattact
    19201 gcaggaaaaa accaaaggcg aactgaaatt aaaactgttc ccggatagca cccttggtaa
    19261 tgcgcaggcg atgatcagcg gcgtgcgtgg cggcactatt gatatggaga tgtctggctc
    19321 taacaacttt acgggactct cgcccgtcat caacctgctc gatgtcccgt tcctgttccg
    19381 cgataccgct cacgcgcata aaacgctcga cggcaaagtc ggtgatgacc tgaaagtctc
    19441 ccttgaaggt aaggggctga aagtgctggc ttactgggaa aacggctggc gcgatgtcac
    19501 caactcgcgt gcaccggtca aaacgcctgc cgacctgaaa ggactaaaaa tccgcaccaa
    19561 caatagcccg atgaatatcg ccgcatttaa agtttttggc gctaacccga tcccgatgcc
    19621 gtttgccgaa gtctataccg ggctggaaac ccgcactatc gacgctcagg aacacccgat
    19681 caacgtcgtc tggtcagcaa aattttatga agtgcagaag tacctttctc tgacccatca
    19741 cgcctattcc ccgcttctgg tggtgatcaa caaagcgaag tttgatggct taaccccgga
    19801 gttccaacag gcactgatct catctgcgca ggaagcgggt agctatcagc gcaaactggt
    19861 cgccgaagat cagcaaaaaa tcatcgacgg tatgaaagaa gcgggcgtgg aagtcatcac
    19921 cgatctcgac cgcaaagcct ttagcgatgc gctgggtact caggtccgtg acatgtttgt
    19981 taaagatgtt ccccagggtg ctgacctgct gaaagccgtg gatgaggtgc aataaaccat
    20041 gacgcaatac tggctggggt tagattgtgg cggtagctgg ctgaaagccg ggctgtatga
    20101 ccgagaaggc cgggaggcgg gcgtgcagcg cctgccgctg tgcgcattaa gtccacagcc
    20161 aggctgggca gagcgcgaca tggcagaact gtggcaatgt tgcactgctg tcattcgcgc
    20221 cctgcttacc cattctggcg tcagcgggga acaaattgtc ggtatcggca tctccgcgca
    20281 gggaaagggc ttatttctac ttgataaaaa cgacaaaccg ctcggtaacg ccattttgtc
    20341 ctcggatcgc cgggcgatgg aaatcgttcg tcgctggcag gaagatggca tcccggaaaa
    20401 actctatccg ctgacccgac aaaccttatg gaccgggcat ccggtgtcgc tgttacgctg
    20461 gctgaaagag cacgaaccag aacgctacgc gcaaattggc tgcgtgatga tgacgcacga
    20521 ctacctgcgc tggtgtttaa ccggcgtcaa aggttgcgaa gagagcaata tttctgagtc
    20581 caatctctac aacatgagtc gcggggaata tgacccgtgt cttaccgact ggctggggat
    20641 tgctgaaatc aatcacgccc tgccgcctgt tgtcggatct gccgaaatct gcggggaaat
    20701 caccgctcag acagccgtac tgaccggtct gaaagcgggt atccccgttg tcggcggcct
    20761 gtttgatgtg gtttccaccg cactctgcgc cggactcgaa gacgaattca ccctcaatgc
    20821 ggtgatgggg acctgggcgg tgaccagcgg cataacccac ggtttacgtg acggtgaagc
    20881 gcatccgtat gtctatggtc gctacgttaa cgatggtcaa tttatcgttc acgaagccag
    20941 ccccacctct tccggcaatc tcgaatggtt taccgcacag tggggagaaa tctcatttgc
    21001 tgagattaat caggccgttg ccagtttgcc gaaagccggt ggcgatctct ttttcctgcc
    21061 gttcctgtac ggcagcaacg ccggactcga gatgaccagt ggtttctacg ggatgcaggc
    21121 cattcacacc cgcgcgcacc tgttgcaggc catctatgaa ggggtggtgt tcagccatat
    21181 gacccatctc aaccggatgc gcgaacgttt tactgatgtg cacaccctgc gcgtcactgg
    21241 cggcccggcg cactccgatg tctggatgca aatgctggcg gacgtcagcg gtctgcgtat
    21301 cgagctgccg caggtggaag aaaccggctg ttttggcgcg gcacttgccg cccgcgtcgg
    21361 caccgggatt tatcacaact tcagcgaagc ccaacgtgac ttacagcacc cggtacgcac
    21421 cctgctgccg aatatggccg cgcatcagct ttaccaacaa aaataccaac gctatcaaca
    21481 tctcattgcc gcacttcagg gttatcacgc ccgcatcaag gagcacacat tatgagtcga
    21541 ccacttctgc aactggccct cgaccactca tcacttgaag ccgcgcagcg cgacgtgacg
    21601 cggttaaaag acagcgtcga tattgtcgaa gcaggcacca ttctctgttt aaacgaaggg
    21661 cttggcgccg tgaaagcctt gcgcgaacag tgccctgaca aaatcatcgt tgctgactgg
    21721 aaggtcgccg acgcgggtga aacgctcgcg caacaggcat ttggcgcagg cgctaactgg
    21781 atgaccatca tctgcgccgc gccgctcgcc acggtagaaa aaggccacgc gatggcacag
    21841 aactgcggtg gtgaaattca gatcgagcta ttcggtaact ggacgctgga cgatgcccgt
    21901 gactggcatc gtattggcgt gcggcaggcc atttatcatc gtggtcgtga cgcgcaggcc
    21961 agcgggcaac agtggggcga ggccgatctg gcacgcatga aggcgctttc cgatatcggc
    22021 cttgagcttt ccattaccgg cgggattacc cctgctgacc tgccgctgtt taaagagatc
    22081 cgcgtgaaag cgtttattgc cggacggtcg ctggcaggcg cagcaaatcc ggcgcaagtg
    22141 gccgctgatt ttcatgcgca aatcgactcc atctggggag gcaagcgtgc gtaatcatcc
    22201 gttaggcatt tatgaaaaag cgctggcgaa agatctctcc tggccggagc ggctggtgct
    22261 ggcaaaaagc tgtggttttg attttgtcga aatgtcggtg gatgaaaccg acgaacggtt
    22321 gtcacgtctt gactggagcg ccgcacaacg tgcttcgctg gtcactgcga tgatcgaaac
    22381 gggtgttgct attccgtcaa tgtgcctgtc cgcacatcgt cgttttcctt ttggtagccg
    22441 ggacgacgcc gtgcgccagc gggcgcggga gattatgagt aaagcgattc gcctggcgcg
    22501 cgatctcggc attcgtacta ttcaactggc aggttacgac gtctattacg aggatcatga
    22561 cgaaggcacc cagcaacggt ttgctgaagg gctggcgtgg gcggttgaac aggcagcggc
    22621 atcacaagtc atgctggcgg tggagattat ggacaccgcg tttatgaact ctatcagcaa
    22681 atggaaaaag tgggacgaaa tgcttgcgtc accgtggttc accgtttacc cggacgtagg
    22741 caacctcagc gcctggggca atgatgttcc cgccgaactg aaactgggca tcgaccgtat
    22801 cgccgccatc cacctgaaag acactcaacc cgttaccaaa catagccccg gacagttccg
    22861 cgatgtgccg tttggcgaag gctgtgtcga ttttgttggc atcttcaaaa cgctgcatga
    22921 gctgaactat cgcggttctt tcctgattga gatgtggacc gaaaaagcca aagagccggt
    22981 gctggagatt attcaggcgc ggcgctggat tgaagcgcgt atgcaggagg ccggatttat
    23041 atgttagagc aactgaaagc tgacgtgctg gcggcaaacc tggcgcttcc cgttcatcat
    23101 ctggtgacgt tcacctgggg caatgtcagc gcgatagacg aaacgcggca atggatggtg
    23161 attaagcctt ccggcgttga gtatgaggta atgaccgccg acgatatggt ggtggttgag
    23221 atagccagcg gcaaggtggt ggaaggcagc aaaaagccct cttccgacac gccaacgcat
    23281 ctggcgcttt accgtcgctt tgcggagatt ggcggcatag tgcataccca ctcgcgccac
    23341 gccaccatct ggtcgcaggc cggacttgat ctccccgcct ggggcactac tcacgccgat
    23401 tatttttacg gagccatccc ctgcacgcgc ctgatgaccg cagaagagat caacggcgag
    23461 tatgaatacc agaccggcga agtgatcatt aaaaccttcg aagagcgtgg gttgaacccg
    23521 gcaaaaatcc cggcggtact ggtgcattct cacggcccgt tcgcctgggg aaaaaatgcc
    23581 gccgatgcgg tgcataacgc cgtggtactg gaagagtgcg cctatatggg tctgttctcg
    23641 cgccagcttg cgccacagct ccctgcgatg caaaacgaac tgctggataa gcactacctg
    23701 cgtaagcatg gggccaatgc ctattacggg cagtagccat acctgatagt gcccggcaat
    23761 acacgccggg cagcctttca catcagaaca gccccaacgg tttatccgag tagctcacca
    23821 gcaggcactt ggtttgctgg taatgctcaa gcatcatttt gtgggtttcg cggccaatgc
    23881 ctgattgttt gtagccgcca aacgccgcat gtgccgggta agcgtgataa cagttggtcc
    23941 acacgcgccc ggcctgaatg ccgcgcccca tcttataggc cagattaccg ttgcggctcc
    24001 agacgcccgc gcccaggcca tattgcgtat cgttcgccag ctccagcgct tcttccatcg
    24061 ttttgaaggt ggttaccgcc agcaccgggc caaaaatctc ctcctggaac acgcgcatat
    24121 tgttctgacc aaacaaaatc gtcggttcga ggtagtagcc gtctttcagt tcgccttcca
    24181 gcagcttgcg ccgcccgccg gtgagcacat cagcgccctc ttttttaccg atatcaatgt
    24241 agttgaggat ggtttccagt tgcccgtgag aaacctgcgc gcccatttgc gtcacgctgt
    24301 cgagcgggtt accgctgcga atgctttcga cacgacggat ggcgcgttcc ataaagcgtt
    24361 cgtagataga ctcctgcact aaagcacggc ttggacaggt gcaaacttcg ccctggttaa
    24421 aggcaaacag tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct tcatccatca
    24481 catcagcaaa gaagatattt ggcgatttgc cgcccagctc cagcgtcacc ggaataatgt
    24541 tttgcgtggc gtattgcata atttgttgac ccacttccgt tgagccggta aacgccactt
    24601 tggcgatgcg tttcgaggtc gccagatatt cgccaatttc cccacctgcg ccgttgacca
    24661 cgttcaccac ccctggcggc agtaaatcac cgacgatttc cattagcagc agtacagaaa
    24721 gcggagtaag acgtgcgggt ttcagcacca cacagttgcc cgccgccaga gcgggagcca
    24781 ttttccagct cgccatcagc agcgggaagt tccacgggat aatctgcccc accacgccta
    24841 acggttcatg gaaatgatag gccacggttt cgctatccac ttcactgatc ccaccttcct
    24901 gcgcccgaat acacgaggcg aaatagcgaa aatgatcaat cgccagcggc acgtcggcag
    24961 cactggtttc gcgaatgggt ttgccgttat cccaggtttc tgccgtcgct aaaagttcaa
    25021 ggttttgttc cattctatcg gcaatcttaa acagaatcgc cgcgcgatcc tgcaccgagg
    25081 tgtgcgccca tttatctttc actttatgcg cagcatccag cgccagatcg atgtctcgtt
    25141 tgcccgaaga cgccacttcg cacagtagct gcccggtcac cggcgtcagg ttctggtaat
    25201 actcgccatc ggcaggcgct acccattcgc caccgataaa gttgtcgtag cgggctttta
    25261 atttgagggg gaaaccatac tcgccgggct taatctgtgc tgaaggggga ttattggtca
    25321 tgatcgtctc cttgcggtgt gaggtataac aagggtagac gtgactggcg aaatcttcgc
    25381 cagtcggtaa cagctttacg acggctatca cgaatttacg ggcaaaagct acagcgggta
    25441 gtgtgcgacg tacactgctt gctggttatt aattcttttc tcttgcgcga atccttcttt
    25501 aacaattgtc cgtaaatcag ctcgagccgt attatcagag acgcccaagc tcgcgctaat
    25561 ttcagcaact gtgtactctt tccccggatt ggcgagcatc acattcagga gcgttacctg
    25621 gcgcggattc aggcgtttaa gcacaccaga gctaaacagc acactatcga gttttgcgct
    25681 tcgttgtgta atttcatcga tatgctgctg ccagttttgc agagcacgtt taatgacacc
    25741 cgcctgatat tcaaggaaat aagtcagatc cataccatct gattcagtgt actggtagct
    25801 ggcggcatat ttgaccggtg cagcatgaag caaacggctg atggaaatat atttaaagac
    25861 atcgtagccc gatttcagca tataccagta aaacagcgcc cgactggtgc gcccgttgcc
    25921 atcccgaaac ggatgttcat gggccagcat aaaatgcaga atgcaggcac ggaccaacgg
    25981 atgaatatac ccttcatggc tgtttaacca ctggcaaact ttttccagcc gttccggtaa
    26041 caatgccgca gcgggcggtt gatgcactat attgccatca tagtcggcga tcacaacatc
    26101 atctgtttca cgaaattctc ccggacgata tttcgcgtca ttgattccac ccataccaat
    26161 ggcatgtaac tggcgaataa gcgcaggtgt cagcggttct gcgagtaagt gcggaatttc
    26221 cgccatcagc cgggcatttc ccgcaatcat atgctcgtct tcagtgcgtg gcgaccgccc
    26281 tgtttccagc atttttctcg ccaccagcgt tgtagtattc gcaccttcca gctgggagct
    26341 ggtgatcggc tcttccaggc gtaactggct taactcggct ccggccccgc gtaaccgttc
    26401 agtctgatct ttcagcgcca gcgcggtagc acgtttgtcg acaagctcgc acgcttctgc
    26461 aatcaccggg gtaatgttga atccggcctg ttcacccgct tcattgcaat agttaattcg
    26521 ttggatcgcc gcgttgcgcg ccagacgggt aagcgtccac gccacatcga cgttttctcc
    26581 ttttttgatg cggtactgca actcatcaaa aggcagataa cgtccttttt catcggtaac
    26641 ccggttaaag cgcaggtggg ccaggagttc ctcacccgat aagtgtgata aaggaataag
    26701 tccgggaggt gatttgatag ccatgtgctt atctcaaaaa agcgcaatat tgagatagtg
    26761 tatagcagga aagagaaact atctcaaaaa aagcaagttt tgagatagtt gaaaaagcgg
    26821 ctgacaactc gccagccgtt gtggaaatga tgattacatc gctgcgcgat aaatcgccac
    26881 aatttcttcg tgagttgcct ggatcgggtt agtaaagccg caggcatctt tcagggcatt
    26941 agtcgccaga accgcgaaat cttcttcttt cacgttcagg tcgcgtaggc ctgccgggat
    27001 atccactttc ttcgccagtt cacggatggc gttaatgcag gcttcagcac cttccgcgtc
    27061 gtttttacct gtcacgttca cgcccattgc agcggcacaa tcacgcagac gtgcagcggc
    27121 gactttgctg ttgaatacct gaacatgcgg cagcaaaacg gcgttacata caccgtgtgg
    27181 caggttgtag aaaccgccca gttggtgcgc catcgcatga acataaccca gagaagcatt
    27241 attgaacgcc ataccggcga ggaactgggc ataagccatt gcttcacgcg ctttcgcatt
    27301 actgccatct tcaacggcta acggcaggtt ttcggcaatc atggtcacgg ctttcagtgc
    27361 acaagcgtca gtgatcggcg tggcggcaat agaaacatac gcttcgatag cgtgcgttaa
    27421 ggcatccata cccgttgcgg cggtcagtga cttcggcata ccaatcatca gagaggagtc
    27481 attgacagaa agcagcggag ttacatgttt atccacaatc gccattttga tgtgacgcgc
    27541 ttcgtcagtg atgatgcaga aacgggtcat ttcagaggcc gtacccgccg tggtgttgat
    27601 ggcgatcatc ggcagctgcg gttttgcaga gcggtcaacg ccttcataat cgcggatgtc
    27661 accgccgttg gctgcaacca gcgcaatacc ttttgcgcag tcgtgtggag aaccaccgcc
    27721 taaagaaatc acgctatcgc aattattttc tttaagtaat ttcaaacctg cggcgacgtt
    27781 ttccgtggtg gggttaggtt gggtgccatc ataaataacg ctaaaaatat tgcgttcttc
    27841 cagtgctttt tgcacatcac ccgccatacc caatttcgtt aacatattgt cggtgacaat
    27901 taaggtacgg gtaaatccat aatctgccat catattcatt gcatcattca atgaatcagc
    27961 accaataaca ttcacggaag gaataaaaaa ggttgaagct gccataacac actcctgaaa
    28021 gtgtaaaaaa gggcgggcag cttactgagg attttcattt gaaaatatga tcaacattac
    28081 cgtttatt
//
LOCUS       DAZKHK010000067        28016 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKHK010000067 DAZKHK010000000
VERSION     DAZKHK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.067"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..701
                     /gene="bcsQ"
                     /locus_tag="ACHOS5_002830"
     CDS             <1..701
                     /gene="bcsQ"
                     /locus_tag="ACHOS5_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP9777621.1"
                     /translation="TAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAM
                     LDGQDWCDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQW
                     ILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGSQ
                     VQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEFRSDALAAEEILTLANWCL
                     LNYSGLKTPVGSAS"
     gene            698..3316
                     /gene="bcsA"
                     /locus_tag="ACHOS5_002831"
     CDS             698..3316
                     /gene="bcsA"
                     /locus_tag="ACHOS5_002831"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP9777622.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3327..5666
                     /gene="bcsB"
                     /locus_tag="ACHOS5_002832"
     CDS             3327..5666
                     /gene="bcsB"
                     /locus_tag="ACHOS5_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP9777623.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5673..6779
                     /gene="bcsZ"
                     /locus_tag="ACHOS5_002833"
     CDS             5673..6779
                     /gene="bcsZ"
                     /locus_tag="ACHOS5_002833"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP9777624.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTQRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6761..10234
                     /gene="bcsC"
                     /locus_tag="ACHOS5_002834"
     CDS             6761..10234
                     /gene="bcsC"
                     /locus_tag="ACHOS5_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP9777625.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPHAQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGVKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDDKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDNGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10316..12304
                     /gene="hmsP"
                     /locus_tag="ACHOS5_002835"
     CDS             10316..12304
                     /gene="hmsP"
                     /locus_tag="ACHOS5_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP9777626.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12487..13773
                     /gene="dctA"
                     /locus_tag="ACHOS5_002836"
     CDS             12487..13773
                     /gene="dctA"
                     /locus_tag="ACHOS5_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP9777627.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13993..15489
                     /gene="yhjJ"
                     /locus_tag="ACHOS5_002837"
     CDS             13993..15489
                     /gene="yhjJ"
                     /locus_tag="ACHOS5_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9777628.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15585..16514)
                     /gene="kdgK"
                     /locus_tag="ACHOS5_002838"
     CDS             complement(15585..16514)
                     /gene="kdgK"
                     /locus_tag="ACHOS5_002838"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP9777629.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16746..17513
                     /gene="pdeH"
                     /locus_tag="ACHOS5_002839"
     CDS             16746..17513
                     /gene="pdeH"
                     /locus_tag="ACHOS5_002839"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP9777630.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17583..19643
                     /gene="yhjG"
                     /locus_tag="ACHOS5_002840"
     CDS             17583..19643
                     /gene="yhjG"
                     /locus_tag="ACHOS5_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9777631.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRKLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQVFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19684..21006)
                     /gene="yhjE"
                     /locus_tag="ACHOS5_002841"
     CDS             complement(19684..21006)
                     /gene="yhjE"
                     /locus_tag="ACHOS5_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9777632.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFAVMVPLAGLLADAFGRRKSMVVITTLIILFALF
                     AFNPLLGSGNPALVFVFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21399..22412)
                     /gene="yhjD"
                     /locus_tag="ACHOS5_002842"
     CDS             complement(21399..22412)
                     /gene="yhjD"
                     /locus_tag="ACHOS5_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP9777633.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22461..23360)
                     /gene="rcdB"
                     /locus_tag="ACHOS5_002843"
     CDS             complement(22461..23360)
                     /gene="rcdB"
                     /locus_tag="ACHOS5_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9777634.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LAMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23864..24466
                     /gene="yhjB"
                     /locus_tag="ACHOS5_002844"
     CDS             23864..24466
                     /gene="yhjB"
                     /locus_tag="ACHOS5_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9777635.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYRLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24517..26166)
                     /gene="treF"
                     /locus_tag="ACHOS5_002845"
     CDS             complement(24517..26166)
                     /gene="treF"
                     /locus_tag="ACHOS5_002845"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9777636.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26571..27968
                     /gene="ccp"
                     /locus_tag="ACHOS5_002846"
     CDS             26571..27968
                     /gene="ccp"
                     /locus_tag="ACHOS5_002846"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP9777637.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         6634 a         7085 c         7729 g         6568 t
ORIGIN      
        1 tcaccgccgc attagcctgg tcattacaaa tgttgggaga aaatgtcctg gtggtcgatg
       61 cctgcccgga caacttgttg cgcctgtcat ttaacgttga ttttacccac cgtcagggtt
      121 gggccagagc tatgctggat ggccaggact ggtgtgacgc cgggttacgc tatacctcgc
      181 agctcgattt gctgcctttt ggtcagttat ccattgaaga acaagaaaat ccacagcact
      241 ggcaaacccg gctgagcgat atttgctccg gcttacagca actaaaagcc agcgggcgtt
      301 accagtggat tttaattgac ttaccgcgtg atgcctcgca gataacccac caactgctga
      361 gtttgtgcga tcactcgctg gcaatcgtca atgtggatgc caactgccat atccgactgc
      421 atcagcaagc gctgccggat ggtgcacata ttttgattaa tgacttccgt attggcagcc
      481 aggttcagga cgatatttac cagctttggt tgcaaagcca gcgccgattg ctgccgatgc
      541 tcattcatcg tgatgaagcg atggctgaat gtctggcagc taagcaacca gtcggtgaat
      601 ttcgcagtga tgcgctggcg gctgaagaaa tactgacgct ggctaactgg tgcctgttaa
      661 actattccgg gctgaaaacg ccagtcggga gtgcatcatg agtatcctga cccggtggtt
      721 gcttatcccg ccggtcaacg cacggcttat cgggcgttat cgcgattatc gtcgtcacgg
      781 cgcgtcggct ttcagcgcga cgctcggctg tttctggatg atcctggcct ggatttttat
      841 tccgctggag cacccgcgct ggcagcgtat tcgcgcagaa cataaaaacc tgtatccgca
      901 tatcaacgcc tcgcgtccgc gtccgctgga cccggtccgt tatcttattc aaacatgctg
      961 gttactgatc ggtgcatcgc gcaaagaaac gccgaaaccg cgcaggcggg cattttcagg
     1021 tctacaaaat attcgtggac gttaccatca atggatgaac gagctgcctg agcgcgttag
     1081 ccataaaaca cagcatcttg atgagaaaaa agagctcggt catttgagtg ccggggcgcg
     1141 gcggttgatc cttggtatca tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac
     1201 tcagccgttt aacccgctag cgcagtttat cttcctgatg ctgctgtggg gggtagcgct
     1261 gatcgtacgg cggatgccgg ggcgcttctc ggcgctgatg ctgattgtgc tgtcgctgac
     1321 cgtttcttgc cgttatatct ggtggcgtta cacctcgacg ttaaactggg acgatccggt
     1381 cagcctggtg tgcgggctta ttctgctgtt cgccgaaacg tacgcgtgga ttgtgctggt
     1441 gctcggctat ttccaggtcg tatggcctct gaatcgtcag ccggtgccat tgccgaaaga
     1501 tatgtcgctg tggccgtcgg tggatatctt tgtcccgact tacaacgaag atctcaacgt
     1561 ggtgaaaaat accatttacg cttcgctggg tatcgactgg ccgaaagaca agctgaacat
     1621 ctggatcctc gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt
     1681 gaaatatatc gcccgtacca ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct
     1741 gaaatatgcc aaaggcgagt tcgtgtcgat attcgactgc gaccacgttc ccacacgttc
     1801 gttcctgcaa atgaccatgg gctggttcct gaaagagaaa cagctggcga tgatgcagac
     1861 gccgcaccac ttcttctcgc cggatccgtt tgaacgcaac ctggggcgtt tccgtaaaac
     1921 accgaacgaa ggcacgctgt tctatggtct ggtgcaggac ggcaatgata tgtgggatgc
     1981 cactttcttc tgcggctcat gtgcggtgat tcgccgtaag ccgctggatg agattggcgg
     2041 tattgctgtc gaaacggtaa ccgaagatgc gcacacttct ctgcgtctgc accgtcgtgg
     2101 ctacacctcc gcgtatatgc gtattccgca ggcagcgggg ctggcgaccg aaagtctctc
     2161 ggcgcatatc ggtcagcgta ttcgctgggc gcgcgggatg gtgcaaatct tccgtctcga
     2221 taacccgctc accggtaaag ggctgaagtt tgctcagcga ctgtgctatg tcaacgccat
     2281 gttccacttc ttgtcgggca ttccacggct gatcttcctg actgcgccgc tggcgttcct
     2341 gctgtttcat gcctacatca tctacgcgcc agcgttgatg atcgccctgt ttgtgctacc
     2401 gcatatgatc catgccagcc tgactaactc gaagatccag ggcaaatatc gccactcttt
     2461 ctggagtgaa atctacgaaa cggtgctggc gtggtatatc gcaccaccga cgctggtggc
     2521 gctgattaac ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga
     2581 agagtacgtc gactgggtga tctcgcggcc ctacatcttc cttgttctgc tcaacctggt
     2641 gggcgtagcg gttgggatct ggcgctactt ctatggtccg ccaaccgaga tgctcaccgt
     2701 ggtcgtcagt atggtgtggg tattctacaa cctgattatt ctcggcggcg cagtcgcagt
     2761 atcggtagaa agcaaacagg tacgccgatc gcaccgcgtg gagatgacga tgcccgcagc
     2821 aattgcccgc gaagatggtc acctcttctc gtgtaccgtt caggatttct ccgacggtgg
     2881 tttggggatc aagatcaacg gtcaggcgca gattctggaa gggcagaaag tgaatctgtt
     2941 gcttaaacgc ggtcagcagg aatacgtctt cccggctcag gtggcgcgcg tgatgggtaa
     3001 tgaagttggg ctgaaattaa tgccgctcac tacccagcaa catatcgatt ttgtgcagtg
     3061 tacgtttgcc cgtgcggata catgggcgct ctggcaggac agctacccgg aagataagcc
     3121 gctggaaagt ctgctggata ttctgaagct cggcttccgt ggctaccgcc atctggcgga
     3181 atttgcgcct tcttcggtga agggcatatt ccgtgtgctg acttctctgg tttcctgggt
     3241 tgtatcgttt attccgcgcc gcccggagcg gagcgaaacg gcacaaccat cggatcaggc
     3301 tttggctcaa caatgatgat aacgcgatga aaagaaaaat attctggatt tgtgcagtgg
     3361 ctctggggat gagtgcgttc ccctctttca tgacgcaggc gacgccagca acgcaaccac
     3421 tgatcaatgc tgagccagct gtagccgccc aggcggagca aaatccgcag gtggggcaag
     3481 tgatgccggg cgtgcagggc gcagatgcgc cagtcgtggc gcagaacggt ccttcccgtg
     3541 atgtgaagct gacctttgcg caaattgcgc cgccgccggg cagcatggtg ctacgtggca
     3601 ttaacccgaa cggcagcatt gagtttggta tgcgcagcga tgaagtggtg acgaaggcga
     3661 tgctcaacct cgaatacacg ccatcgccat cattactgcc tgtccagtcg cagttaaagg
     3721 tttatctcaa tgatgaactg atgggcgtgc tgccagtgac caaagaacag ttgggtaaaa
     3781 aaacgctggc acaaatgccc attaacccgc tgtttattac tgatttcaac cgtgtgcggc
     3841 tggagtttgt cggccattat caggacgtgt gcgaaaaccc ggccagcacc acgctttggc
     3901 tggatgttgg gcggagcagt ggactggatc tgacctatca gaccctgaat gtgaagaatg
     3961 acctgtcaca cttcccggtg ccattctttg acccgcgtga taaccgcacc aacaccttgc
     4021 cgatggtctt tgcgggtgcg ccggatgttg agctgcaaca agcatctgcc attgtcgcct
     4081 cgtggtttgg ttcgcgttct ggctggcgtg ggcagaactt cccggtgctc tataaccaac
     4141 tgccggatcg caatgccatt gtctttgcca ccaatgacaa acgcccggac ttcctgcgcg
     4201 atcatccggc ggtaaaagcc ccggtgattg agatgattaa ccatccgcag aatccttacg
     4261 tcaaactgct ggtggtgttt ggtcgtgacg acaaagactt gttgcaggca gcgaaaggta
     4321 tcgctcaggg taacattctg ttccgtggtg aaagtgtggt agtgaatgaa gtgaaaccgc
     4381 tgctaccacg taagccgtac gatgcgccga actgggtgcg taccgatcgt ccggtcacct
     4441 ttggtgaact gaaaacctat gaagaacaat tacaatccag cggtcttgag ccagcagcga
     4501 ttaacgtttc gctaaacctg ccgccggatc tctacctgat gcgcagtacc ggcattgata
     4561 tggatattaa ttaccgctac accatgccgc cggtgaaaga cagttcgcgg atggatatca
     4621 gcctgaataa ccagttcctg caatccttca acctgagcag caaacaggag gcgaaccgcc
     4681 tgctgctgcg gattccggta ttacaaggtt tgctggatgg caaaacggat gtctctattc
     4741 cggcgctgaa actgggcgcg accaaccagc tgcggttcga ctttgagtat atgaacccga
     4801 tgccgggcgg ttcggtggat aactgcatta ccttccagcc ggtgcagaat catgtggtga
     4861 ttggtgacga ctccaccatc gacttctcga agtattacca cttcatcccg atgccggatc
     4921 tacgcgcctt tgctaacgcg ggcttcccgt tcagccggat ggcggatttg tcgcaaacca
     4981 tcaccgtgat gccgaaaacg ccaaacgaag cacagatgga aaccttgctg aataccgttg
     5041 gctttatcgg tgcgcagacg ggcttcccgg cgattaacct gacggtgacc gatgatggca
     5101 gcaccattca gggcaaagat gccgacatca tgatcgtcgg tggtatcccg gacaaactga
     5161 aagacgataa gcagatcgac ctgttggtgc aggctaccga aagctgggtg aaaacgccga
     5221 tgcgccagac cccgttcccc ggtattgtgc cggacgagag cgatcgcgcg gcagaaaccc
     5281 agtcaacgct gacctcttcc ggtgcgatgg cggcggtgat tggcttccag tcgccgtata
     5341 acgaccagcg cagcgtgatt gcgctgctgg cagatagccc acgcggttat gaaatgctta
     5401 acgatgcggt gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc
     5461 gcgagtccgg tatcaacagc ctacgtgttg gcgatgttta ttacgtaggt catctgccgt
     5521 ggttcgagcg cttgtggtat gcgctggcaa accatccgat tctgctggcg gtgctggcgg
     5581 ctatcagtgt gatattgctg gcgtgggtac tgtggcgtct gctgcgtatt attagtcgtc
     5641 gtcgtcttaa cccggataac gagtaattga agatgaatgt gttgcgtagt ggactcgtga
     5701 cgatgctgct gctggctgcc tttagtgttc aggcagcctg tacctggcct gcctgggagc
     5761 agtttaaaaa ggattacatc agtcaggaag ggcgcgtcat cgaccccagc gacgcgcgca
     5821 aaatcaccac ctccgaaggg caaagttacg gcatgttctt tgccctggcg gctaacgacc
     5881 gtgcagcttt cgataatctt ctcgactgga cgcagaacaa tctcgctcag ggttctttaa
     5941 aagaacattt gcccgcctgg ctgtggggca agaaagagaa cagtaagtgg gaagtgctgg
     6001 acagcaattc ggcctccgat ggtgatgtct ggatggcctg gtcgttgctg gaggcggggc
     6061 gtttgtggaa agagcagcgt tataccgaca tcggcagcgc attgctaaaa cgtatcgcgc
     6121 gggaggaagt ggtgacggtg cctgggctgg gctccatgtt gttaccgggc aaagtgggtt
     6181 ttgctgagga taacagctgg cgttttaacc ccagctacct gccgccgacg ctggcgcagt
     6241 atttcacccg ctttggcgcg ccgtggacca cgcagcgcga aaccaatcaa cgtttattgc
     6301 tggaaaccgc cccgaaaggc ttttcgccag actgggtgcg ctatgagaaa gacaaaggct
     6361 ggcagctaaa agccgaaaaa acattgatca gcagctacga cgctatccgc gtttacatgt
     6421 gggtaggcat gatgcctgac agcgatccgc aaaaagcgcg gatgctcaac cggtttaaac
     6481 cgatggcgac attcactgag aaaaacggtt atccgccgga aaaagtggat gtggctacgg
     6541 ggaaagcgca gggtaaagga ccggtcggtt tttctgccgc catgctgccc tttttacaaa
     6601 accgcgatgc gcaggccgtt cagcgccagc gcgtggccga taactttccc ggcagcgatg
     6661 cctattacaa ctatgtgctg accctgtttg gacaaggctg ggatcaacac cgtttccgct
     6721 tctcgacaaa aggtgagtta ttacctgact ggggccagga atgcgcaaat tcacactaaa
     6781 catattcacg ctttccctcg gtctggccgt catgccgatg gtcgaggcag caccaaccgc
     6841 tcagcaacag ttgctggagc aagttcggtt aggcgaagcg acccatcgtg aagacctggt
     6901 gcaacagtcg ttgtatcggc tggaacttat tgatccgaat aacccggatg tgattgctgc
     6961 ccgttttcgt tctctgttac gtcagggcga tattgatggc gcacaaaaac agctcgatcg
     7021 gctgtcgcag ttagcgccga gttcaaatgc gtataaatcg tcacggacta cgatgctgct
     7081 ttccacgtcg gatggtcgtc aggcactgca acaggcaaga ttgcaggcga cgactggtca
     7141 tgcggaagaa gctgtggcga gttacaacaa actgttcaac ggtgcgccgc cggaaggtga
     7201 cattgctgtc gagtactgga gtacggtggc gaaaattccg gcccgccgtg gcgaagcgat
     7261 taatcagcta aaacgcatta atgcggatac gccgggcaac acgggcctgc aaaacaatct
     7321 ggcgctatta ctgtttagta gcgatcgccg tgacgaaggt tttgccgtcc tggaacagat
     7381 ggcgaaatcg aacgccgggc gcgaaggggc ctctaaaatc tggtacgggc agattaaaga
     7441 catgcctgtc agcgatgcca gcgttcaggc actgaaaaaa tatctctcga tctttagcga
     7501 tggcgatagc gtagctgctg cacaatcgca actggcagaa cagcaaaaac agctggccga
     7561 ccctgctttc cgcgctcgtg cgcaaggttt agcggcggtg gactctggca tggcgggtaa
     7621 agccattccc gaactacaac aggcggtgcg ggcgaacccg aaagacagtg aggctctggg
     7681 ggcgctgggc caggcgtatt ctcagaaagg cgatcgcgct aatgcggtgg cgaatctgga
     7741 aaaagccctc gcgctggatc cgcacaacag caataacgac aaatggaaca gtctgctgaa
     7801 agtaaaccgc tactggctgg cgatccagca gggcgatgcc gcgctgaaag ccaataatcc
     7861 tgaccgggca gaacggctgt tccagcaggc acgtaatgtc gataacaccg acagctatgc
     7921 agtgcttggg ctgggcgatg tggcgatggc gcgcaaagat tatcccgccg ccgaacgcta
     7981 ttaccagcag accttgcgta tggacagcgg caacactaac gccgtgcgcg ggctggcgaa
     8041 tatttaccgc cagcagtcgc cagaaaaagc cgaagcgtat atcgcctcgc tctctgccag
     8101 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
     8161 ggcagaggca ctggaaaacc aggggaaatg ggcgcaggcg gcagcacttc agcggcaacg
     8221 actggcgctg gaccccggca gtgtatggat tacttaccga ctttcgcagg atctctggca
     8281 agccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
     8341 cgacccggag caggtttacg cttacgggct gtacctctcc ggtcatgatc aggacagagc
     8401 ggcgctggcg catatcaaca gcctgccgca cgcgcagtgg aacagcaata ttcaggagct
     8461 ggttaatcga ctgcaaaacg atcaggtgct ggaaaccgct aatcgcctgc gagaaaacgg
     8521 taaagaggca gaagcggaag cgatgctgcg ccagcaaccg ccttcctcgc gtattgatct
     8581 gacgctggct gactgggcgc agcagcgacg tgattacacc gccgcccgcg ctgcatatca
     8641 gaatgttctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
     8701 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccactga
     8761 taacgcctcg ctgaacacac agcggcgcgt ggcgctggca caggcgcagc ttggcgatac
     8821 cgcagcggcg cagcagacat ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
     8881 gatggaaagc gcgatggtac tgcgtgatgg tgtgaagttt gaagcgcagg cgggcgatcc
     8941 aacgcaggcg ctggaaacct acaaagacgc catggttgca tccggtgtga ccacgacgcg
     9001 tccgcaggat aacgacacct ttacccggct gacccgtaac gacgataaag atgactggct
     9061 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
     9121 tctcgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
     9181 caccactatg ttgcaggtgg acgcgcccta ttctgacggg cggatgttct ttcgcagtga
     9241 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
     9301 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
     9361 cagcgtggcg gtcggctggc gaaatgacgt ctggagctgg gatatcggta ccacgccgat
     9421 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
     9481 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
     9541 gcaaaaagac tctccgagca ataccgggaa aaaatggggc ggcgtgcgtg ccgacggcgt
     9601 ggggctaagt ctgagctacg ataacggtga agcaaacggc gtctgggcat cgcttagcgg
     9661 tgatcagtta accggtaaaa atgtcgaaga taactggcgt gtgcgctgga tgacgggcta
     9721 ttactataag gtcatcaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
     9781 ctggcattac gacaaagacc tgagtggcta ctcactcgga cagggcggtt actacagtcc
     9841 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
     9901 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
     9961 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
    10021 tggcggcagc agtcagggct ttggctacac ggcgcgggca ttacttgaac gacgtgttac
    10081 ttccaactgg tttgtcggca cggcaattga tatccagcag gcaaaagatt acgcacccag
    10141 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    10201 accgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    10261 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    10321 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    10381 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    10441 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt ctgccatttt
    10501 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg ccggcgtggt
    10561 cagccgtgcc gatgtagtgc tgcccaacca gttccaggcg ctgcgtaaaa gttttattcc
    10621 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggtgc aaatctcgct
    10681 gggcgtttac tcgctggaac gtccggcaaa cccgcagcca attgcctatc tggtgttaca
    10741 ggcggattcc ttccgcatgt ataagttcgt gatgagcacc ctctcaacgt tagtgaccat
    10801 ttacttactt ttgtcgttaa tattgacggt ggcgattagc tggtgcatta accgcctgat
    10861 tttgcatcca ttacgcaata ttgctcgcga acttaacgcc atcccacccc aggagcttgt
    10921 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    10981 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    11041 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    11101 ggtggtggcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    11161 tactgcgggt gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    11221 actcaaatcg gtactgtcgc cccgcatgat cctcgcgcag attagcggtt atgactttgc
    11281 tgtcattgcc aacggtgtac aggaaccgtg gcacgctatc acattaggtc agcaagtgct
    11341 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    11401 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    11461 tgcggcattt accgctcgcc ataaaggcaa aaatcagatt cagttctttg atccgcagca
    11521 gatggaagcc gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    11581 tcatcaattt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    11641 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc ccgatggctt
    11701 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    11761 gtcctgtcga ttgcttgctg cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    11821 cctctcagcg ctgcaactga tgcatccaaa tatggtggcg gatatgctgg aactgttaac
    11881 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    11941 cgatcctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    12001 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    12061 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggtttgccgg aagatagcag
    12121 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    12181 cgtggagact gaagcacaac gcgactggct ggcaaaagcg ggcgttggta ttgcccaggg
    12241 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    12301 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    12361 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    12421 tgttatttta aggccgcagg taccccataa ccttacaaga cctgtggttt tactaaagga
    12481 caccctatga aaacctctct gtttaaaagc ctttactttc aggtcctgac agcgatagcc
    12541 attggtattc tccttggcca tttctaccct gaaataggcg agcaaatgaa accgcttggc
    12601 gacggcttcg ttaagctcat taagatgatc atcgctcctg tcatcttttg taccgtcgta
    12661 acgggcattg cgggcatgga aagcatgaag gcggtcggtc gtaccggcgc agtcgcactg
    12721 ctttactttg aaattgtcag taccatcgcg ctgattattg gtcttatcat cgttaacgtc
    12781 gtgcagcctg gtgccggaat gaacgtcgat ccggcaacgc ttgatgcgaa agcggtagcg
    12841 gtttacgccg atcaggcgaa agaccagggc attgtcgcct tcattatgga tgtcatcccg
    12901 gcgagcgtca ttggcgcatt tgccagcggt aacattctgc aggtactgct gtttgccgta
    12961 ctgtttggtt ttgcgctcca ccgtctgggc agcaaaggcc aactgatttt taacgtgatc
    13021 gaaagtttct cgcaggtcat tttcggcatc atcaatatga tcatgcgtct ggcacctatt
    13081 ggtgcgttcg gggcaatggc gtttaccatc ggtaaatacg gcgtcggcac actggtgcaa
    13141 ctggggcagc tgattatctg cttctacatc acctgtatcc tgtttgtggt gctggtactg
    13201 ggttcaatcg ctaaagcgac tggtttcagt atcttcaaat ttatccgtta catccgtgaa
    13261 gaactgctga ttgtactggg gacttcatct tccgagtcgg cgctgccgcg tatgctcgac
    13321 aagatggaga aactcggctg ccgcaaatcg gtggtggggc tggtcatccc gacaggctac
    13381 tcgtttaatc ttgatggcac atcgatatac ctgacaatgg cggcggtgtt tatcgcccag
    13441 gccactaaca gtcagatgga tatcgtccac caaatcacgc tgttaatcgt gttgctgctt
    13501 tcttctaaag gggcggcagg ggtaacgggt agtggtttta tcgtgctggc ggcgacgctc
    13561 tctgcggtgg gccatttgcc ggtagcgggt ctggcgctga tcctcggtat cgaccgcttt
    13621 atgtcagaag ctcgtgcgct gactaacctg gtcggtaacg gcgtagcgac cattgtcgtt
    13681 gctaagtggg tgaaagaact ggaccacaaa aaactggacg atgtgctgaa taatcgtgcg
    13741 ccggatggca aaacgcacga attatcctct taatctcacc acttatgccc gcactccctt
    13801 ccccgagtgc gggtgtttgc gcataattga cccctgtgcc tgaacttacc acctgggtga
    13861 gttcaggttt atttcacttc ttccgtgaca ttttcatgtt cttgcggtct aacacgaagt
    13921 gtttttaacg tcatttagac tgaccgacaa cgggcagttt tttattacca ggatagttga
    13981 tcaggggttc acatgcaggg cacaaaaatt cgacttttag cgggcggttt gctgatgatg
    14041 gccactgctg gctatgtgca ggcagatgcg ctccagcctg atccagcatg gcaacagggg
    14101 acgctttcca acggtttaca gtggcaagtg ctgactacgc cccaacgtcc cagcgatcgt
    14161 gttgaaattc gcctgctggt taataccggt tcgctcgccg aaagtacaca acagagcggt
    14221 tacagtcacg ccatccctcg tattgcgcta acgcaaagcg gtggccttga cgcagcgcag
    14281 gcgcgttcat tgtggcagca ggggatcgac cctaaacgcc cgatgccgcc ggtaattgtc
    14341 tcttatgaca ccacgctgtt taatctgagt ttgcccaata accgtaacga cttgctgaaa
    14401 gaggcgctct cttatctggc aaatgccact ggcaaactga ctatcacgcc agaaaccatc
    14461 aaccatgcgt tgcaaagtca ggacatggtg gcaacctggc ctgccgatac taaagagggc
    14521 tggtggcgtt atcgtctgaa aggatcaacc ttgttaggtc acgatcctgc cgatccgctg
    14581 aaacaacccg ttgaagcgga aaagattaaa gatttctatc agaaatggta caccccggat
    14641 gcaatgacgc tgctggtggt gggaaacgtg gatgcgcgct cggtcgtcga ccaaatcaat
    14701 aaaacgtttg gcgaactgaa aggcaaacgt gaaacaccgg ctccggtgcc gacgctttct
    14761 ccgctgcgtg cggaagcggt gagtattatg accgacgcgg tgcgtcagga ccggctatct
    14821 atcatgtggg atacgccgtg gcagccgatt cgtgaatcag ccgcactgct gcgctactgg
    14881 cgtgcggacc tggcccgtga ggcgctgttc tggcatgttc agcaagcgtt aagcgccagt
    14941 aacagtaaag acatcggtct tggatttgac tgtcgtgtgc tgtatctgcg tgcgcagtgt
    15001 gccatcaaca tcgaatcacc aaacgacaag ctgaacagca accttaatct ggtggcgcgt
    15061 gaactggcga aggttcgcga taaaggtctg ccggaagaag agttcaatgc gttagtggcg
    15121 caaaagaaac tggagctgca gaaactgttt gccgcctatg cgcgagctga taccgatatt
    15181 ctgatggggc agcggatgcg ttcgttgcaa aatcaggtcg tcgatatcgc gccggagcaa
    15241 tatcagaaac tgcgtcagga tttccttaac agcctgacgg tggagatgtt aaatcaggat
    15301 ctgcgtcagc agttgtcgaa tgatatggcg ttaatactgc tgcagccgaa aggcgagccg
    15361 gaatttaaca tgaaagcgtt gcaggcggcc tgggatcaaa tcatggcccc atcgactgcg
    15421 gctgccgcca catctgtcgc cacggatgac gtacatcctg aagtgacgga tattccacct
    15481 gtacagtaat gcctgatggt gatgagttag cacaaccatc aggtatttat ggatgagctg
    15541 gatagagggg taacactcta tccagccttt tgcatatgct gcttttacgc tggcatcgct
    15601 tcacgcggga taatcgcgcc gcgatactga ataacggtac ttgcggtcag gtgcccgcgt
    15661 ttcgctgcgt cttccgcact gccgcccgtc aaacgtaccg ccagataacc ggcactgaaa
    15721 gagtcgccag ctgcggtggt atcgatcact ttttctttcg gcagttttac cgccggaaca
    15781 tccactaacc cttcgccagc aatggacacc aggcaagaat ccgccccgcg tttcaccacc
    15841 acttctttca cgcccgcatt atgggtgcgc gcaatgacgt cttccaccgg ctgttgaccc
    15901 cacagcgcat cttcatcgtc cagcgtcagg aaggcgatat ccgtgcattc cagcatttgt
    15961 tggtacacct gctgtgtctc ttctttgctg gcccacaggc gcggacgata gttattgtcg
    16021 aaaatcactt ttccgccgtt ggcgcggcat tcgcgcagca gggaaagcag cttttcgcgg
    16081 ctggtcgggc ttaagatcgc caggctaatc ccgctcaggt agagataatc gaaattcgcc
    16141 agctcttcgc aaatcgccgc ggattgctca ctctccagcc agaatttggc ggcggcttcg
    16201 ttacgccagt agtagaacgt acgttcgccg gtgctgtcgg tttcaatgta gtaaaggccc
    16261 ggcagacggt tttccatccg ttgggtcagg gaagtatcaa cgttctcgcc gcgccaggcg
    16321 tccagcatct gctggctaaa actgtccgtt cccagcgccg ttacgtaatg aacggttaat
    16381 gccgcaggat cgacctgacg ggcgatatag acggaagtgt tcagggtatc gccgccgaaa
    16441 ccgcgcttaa cgtccgcgcc tttctcggaa agctcaatca tgcattcgcc aatcacggca
    16501 atctttttgg acatagtcgt gaacctgatc tgtaaaaaat tagcgttagt ttgcgctgtg
    16561 gtggtttgct ggtcaaccat attaaaacag tgttccatta tttttttgag ccagaacgta
    16621 ttcctgaaag attccgttgt ggagtgggga aatttacccc ggccaggcgg taaagttctg
    16681 cccttgcgcg ccgataatct ttgtcgagtc cgggcagcat cacttttaaa cacaggacat
    16741 ctttgatgat aaggcaggtt atccagcgaa taagcaaccc tgaagcaagc atcgagagct
    16801 tgcaggaacg gcgtttttgg ttgcagtgtg agcgtgctta cacctggcag ccgatctatc
    16861 aaacatgcgg gcggttaatg gccgtggagc tattaacggt ggtcacgcat cccttgaacc
    16921 cttcgcaacg cctgccgccg gatcgctatt ttactgaaat caccgttagc catcggatgg
    16981 aggttgtgaa agagcagatt gatttgctgg cgcaaaaagc cgacttcttt gtagagcacg
    17041 gcctgctggc atcggtcaat attgatggcc ctacgctcat cgccctgcgt cagcaaccaa
    17101 aaatcctgcg ccagattgag gatcttccct ggctgcgttt cgaactggtg gaacacatcc
    17161 gcctgccgaa agattcaacc tttgcctcga tgtgtgaatt tggcccgctg tggctggatg
    17221 attttggtac cgggatggca aatttctctg cactaagtga agtgcgttat gactacatca
    17281 aaatcgcgcg agaactgttt gtgatgctgc gtcagtcgcc ggaaggacgc acactctttt
    17341 ctcagctttt acatctaatg aatcgctatt gtcgcggggt gattgtcgag ggcgtagaaa
    17401 caccggaaga gtggcgtgat gttcagaact cgcccgcatt cgccgcacaa ggctggtttc
    17461 tttcacgccc ggcaccgata gaaacgctga atacggcggt tctggcgcta taagctgcct
    17521 cattttccgc ctggctggac tatctttagg actggcacag gaagaatatg aggaagcgaa
    17581 cgatgagcaa ggcaggcaaa ataaccgctg cgatttcagg ggctttcttg ttgttgattg
    17641 tcgtggcgat cattttgatt gcaacttttg actggaatcg actcaaaccg accatcaacc
    17701 agaaagtctc tgcggagttg aatcgtccgt tcgctatccg tggcgatctg ggcgtggtgt
    17761 gggagcggca aaaacaagaa actggctggc gcagctgggt gccgtggccc catgtacacg
    17821 cggaagacat cattcttggc aatccaccgg atattcccga agtcacaatg gtgcatttgc
    17881 cgcgagtaga agcgacgctg gccccgctgg cgctgctgac caaaacggtc tggttgccgt
    17941 ggatcaagct cgaaaagccc gacgcgcgcc tgattcgcct ctccgaaaag aacaataact
    18001 ggacgtttaa tctcgccaac gatgacaaca aagacgcgaa tgcaaagccg tcggcgtggt
    18061 cgtttcagct ggataatatt cttttcgatc aagggcggat cgccattgat gacaaagtaa
    18121 gcaaagcgga tctggaaatt tttgtcgatc cgttaggcaa gccgctgccg ttcagcgaag
    18181 ttaccggatc gaaaggtaaa gcggataaag aaaaggtggg cgattacgtt tttggcctga
    18241 aggcgcaggg acgttataac ggtgaaccgc tcactggtac gggaaaaata ggcggtatgc
    18301 tggcgctgcg tggcgaagga acgccgtttc cggtacaggc tgatttccgc tctggtaaca
    18361 cccgtgttgc ttttgatggc gttgtgaatg acccaatgaa gatggggggt gtcgatttac
    18421 ggcttaaatt ttctggcgat tcgctgggtg atctctatga actgacgggc gttctgctgc
    18481 ccgatacacc gccgtttgaa acggatggtc ggctggtagc gaaaatcgac actgaaaaat
    18541 cgtcggtctt tgattatcgc ggttttaatg ggcgcattgg cgatagcgat atccacggtt
    18601 ctctgatcta taccaccggc aagccacgac caaaactgga aggtgatgtc gagtcgcggc
    18661 aattgcggct ggcggacctg ggaccgttga ttggcgttga ttccgggaaa ggggcagaaa
    18721 agtcgaaacg gtctgaacag aagaagggcg aaaaaagcgt tcagcctgcg ggcaaagtgc
    18781 tgccttatga ccgcttcgaa accgataaat gggacgttat ggatgccgat gttcgcttca
    18841 aagggcggcg cattgagcat ggcagcagcc tgccgattag cgatctttct actcatatca
    18901 tcctcaaaaa tgctgacctg cgcctgcaac cgctgaaatt tggcatagcg ggcggcagca
    18961 ttgcggcgaa tattcatctg gaaggcgata aaaagccgat gcaggggcgg gcagatattc
    19021 aggctcgtcg actgaaactg aaagaactga tgccggatgt ggaactgatg cagaagacgc
    19081 tgggggaaat gaacggtgac gcggaactac gcggtagcgg taactcggtg gcggcgcttt
    19141 taggcaacag taatggcaac ctgaaactgt tgatgaatga cgggctggtg agccgcaaac
    19201 tgatggagat tgtcgggctg aatgttggta actacattgt cggtgcgata tttggtgacg
    19261 atgaggtgcg ggtgaactgc gcggcagcga atctggatat tgccaacggt gtggcgcgtc
    19321 cgcaggtttt cgcttttgat accgagaatg cgttgatcaa tgttaccggc acggcaagtt
    19381 ttgcttcgga acagctggat ttaactatcg atccagaaag taaagggatt cgcattatca
    19441 cgctgcgttc gccgctgtat gtgcgtggga cgtttaaaaa tccgcaggct ggggtgaaag
    19501 ccgggccgtt gattgcccgt ggtgctgttg cggcggcact ggcaacgctg gttacgccag
    19561 cggcagcatt gctggcactg atctcacctt ccgaagggga ggctaatcag tgccggacaa
    19621 ttttgtcgca gatgaagaag tgatgataat tgccggatgt ctgacatccg gcagttaaaa
    19681 cgactacaac gactggtgcc gcgtctcatg cgttaacagc agggcaatta acgtcagtcc
    19741 ggccatcgcc gccagatata accccaccgc gcctaaccca tagttagtct gcaaccaggc
    19801 cgcgatatac ggcgcaacag acgccccgag aatcgacgat acgttatagg agaacgatgc
    19861 tccggtataa cggacttccg tcgggaacag ctccggcagc agcgccccca tcggaccaaa
    19921 ggtcaggccc atcaaactca agcccagcag caggaaaaca aacaccaatg ctggattacc
    19981 agaacccaac agtgggttaa aggcaaacag ggcaaacaga atgataagcg tagtgatcac
    20041 caccatactt ttacggcgac caaaagcatc tgccagcaga cccgccaacg gaaccatcac
    20101 tgcaaaaccg ataaccgcca tcatcaacat ccacagtact tcgttacgcg gtaaaccaag
    20161 gccaacaggc gccgctgcgg tactaaaggt catagagtag accgtcatga tgtaaaacag
    20221 cgtataggtt gccagcataa tgaacgtacc cagtacggtt acgcgaacat gtttggtcag
    20281 cagcgtaccc agcgggatct tcacctgttt tttcgcttta gcgactttct caaacaccgg
    20341 cgactcatgc agcgacacgc gaacatacag gccgataatg accagcaccg ccgagaagat
    20401 aaatggcaca cgccagcccc agctcataaa ctgctcgtcg gtcagtagcc aggaaagcag
    20461 caagaaagtg ccgttggcaa agaagaagcc aatcggtgcg cccaactgcg ggaaggagcc
    20521 gtacaatgca cgtttgcgcg gtggggcgtt ttcagtcgcc agcagcgccg cgccgcccca
    20581 ttcaccacct aagcccagac cctgaccaaa tcgagccagc gccagcagca gcggggcgaa
    20641 aataccaatc gtggcatagc ctggcagcag accaatcacc acggtcgaaa tccccatcgt
    20701 tagcaacgag gcgaccagcg tcgctttacg cccaacgcga tcgccaaaat gaccaaaaac
    20761 ggcagagcca atggggcgcg cgacgaaggc gatggcgaag gtggcgagcg actgtagcgt
    20821 tgctgccgtt ggatcgccct gcgggaagaa gatatgcgga aacacaataa cggccgcagt
    20881 ggcgtaaatg taaaagtcga agaactcaat ggccgtgcca atgagagagg cgacaaggac
    20941 tttattacgc gagttgatcg gcgtgtattc ttgctcgtgg tcgagtgttg tggctgttgc
    21001 ttgcataatc ttttcttatt ttttggctaa cgaatagcca tattacgcac agcaaaagtg
    21061 atatttcaat gtgtaacaaa tcgcggaaat ggtcaaaaat catgcaaaaa ggggataatg
    21121 tttaggccag tgatttttct tctatgaaat gcttcacata attttataaa tggtggatta
    21181 aactggtttt aggttaaata aaagttacca actggattta tatgctgggg ttatgagttg
    21241 gaatgaaatt gtttgctcct gaatagatgg ctaaagaggc tctgggaaag agtttcaaga
    21301 ggttatgtgc gcattatagc cggatgcgat gctggcgcat cttatccggc ctacggggag
    21361 ccaggccgaa taaattcgta cgccgcatcc ggcctatttt aaggctgcgt tttccccggc
    21421 attcgcgggt cgtctttata ttcggcggtg gcaatccacg ccgcgcaaaa cagcgtcaaa
    21481 cgggcgaaga agtagaaaaa tgccatcagc cccagcacgg aaccaaatgc tgcgccagag
    21541 ggtgatttca tcaacgatgg cagggtgtag gtcatcacga ttttaatcac ttcaaaacca
    21601 atagcagcca gaaatgttcc gcggatcagg gcttttttgc gcggacggtg gcgcggcaac
    21661 cgccagaaga tccagaagaa aagcagatag ttggcgaaga tggaaatcgc caggccaatc
    21721 aaccgccatg tcggtttcag ccactcaatg ctgttcaggt gcagggcgct aataatcatt
    21781 tgctgcgcag aaccggcaac cgaggtgata gaaagcgtca caatcagcgc aatcaacaaa
    21841 ccaatcagcg aaataaaatc acgcagatat tttacccaga acttctcctg atcttgcggc
    21901 gagcgttccc agacatcgcg tgactgggcg cgaatcgctt cacgcaggtt acccatccag
    21961 ttgatgccgg aataaagcgc cactgccaga ccgacaagcc ctacagtcgt acgctgctga
    22021 acggcggtgt tgatggtgtt tttcaacgtg gcggctagcg tcggatcgct aatgttttgc
    22081 agaattttgt cgaagatatc ctgtagcaac atcggatggg aggccagcac aaagccccct
    22141 gcggcaaacg acaccatcaa aatggggatc atcgacaaaa atgagaaata ggtgatagcc
    22201 gccccaaact ggttacccag ccgatcgtta aagcgttctg tcgcgcgaat caggtgcgca
    22261 ataaccggtt ggcgttggac tttctcggca gtggtggtga cggtttccag cgcctggcta
    22321 actttgccgc ctttctcgct attatccaac tgcttaatcg gctcgtgctc cagatcctgg
    22381 gtgggacgtt tgatctcgtt ttcctgcgtc atccgctctt ttgtccttct gtcgtggtta
    22441 ttcttaaaaa gtatagtcgg tcagtccagg tgctctttca ttacgccagc cagccactgc
    22501 ataaacaggt ttacacgccg ggaaagctcc cgacgctgcg gataaaccag tgaaagggac
    22561 agcggctcgg cacggtagcc aggcaacact tcaataagcc gcccggcacg cagggcttcg
    22621 cgcacggcaa tgcgcgggat ctgaataatc cccagaccgg caagaccggc ggcgagatag
    22681 ttttcgctac tgtttaccgt caacatgccg ccagacttaa accactggac gccattaacg
    22741 ctggcaacct caaaacctaa cggatgtaca cccaggtgcg gtgtataacg cactattgcg
    22801 tgtgaagtca gatcatcggg gctttgcgga taaccaaagc gcgtcagata gtgtggactg
    22861 gcacagttga ccatggccag tttgccgaga gaacgggcga taacgccatc ttccggtaat
    22921 gcgccagtgc gtatcacgca atcaaaacca tcatgaagaa tatctaccgg acggtcatgg
    22981 ctactcagtt ccagctcaat tcccggatgc agatagagaa attccgacaa gcgcggtagt
    23041 aacaggcttt tcgcgattcc tgacgggatg tcgatgcgta atttaccgct gatactggtg
    23101 gcatcctgtt gaaacagacc gtccagttcg ctgagattac tcaacacatc ttttgctcgt
    23161 tgataatagg tcatgccttc tggcgtgagt ttgacccgtc gcgtggtgcg ctggagaagc
    23221 tgggtgccaa gttgatgttc cagtgcctgt atctggcgcg aaacacttcc ctttggcaaa
    23281 gcaaagaaat ccgctgcgcg ggaaaaactt tccagctccg cgactttgat gaacaactgc
    23341 attgcgtgaa ttttatccat ctctttgacc gattgttgtt cacaacgaaa cagtagcgcg
    23401 catatcgcat tgtttattgt tttttctaac atctaatgcc gggtttcaca atgttgtcat
    23461 gatgaatgtg gcgatttctc agcaggaaaa gggaggaata gctttatgag aaaggtgaat
    23521 tcccttaaac agagcgtatt tttcagcgat tccctttaaa gtgaatagaa gatgtattgg
    23581 ctatttgtgc ttatttttat ctcattgccg tttgttattt ttgtttatgg tcgcggaatt
    23641 tcatttttga ttgcatgcgt actaactacc gatataagga aatcctcaaa aatgttttaa
    23701 atagtgccct cgacatgtaa tttcaggcgt ttagctgtaa ctattttcta cgcgtcatct
    23761 ctgtagaatg gccaccgcca aatggcgaat tcttattttt tctcgcgggt aatacccttc
    23821 cactacttaa gtctttttaa cgtattcagg tggagattca gttatgcaaa tagtcatgtt
    23881 tgacaggcag tcaatattta ttcatggaat gaaaatcagt ttacagcatc gtattccagg
    23941 agtgagtatt cagggggcca gtcaggcaga cgagttatgg caaaagctgg aaagttaccc
    24001 tgaagcctta gtgatgctcg atggcgatca ggatggtgag ttttgctaca ggttgctgca
    24061 aaaaaccgtg gtgcaatttc ctgaggttaa ggtgttaatt acggcgacgg attgcaacaa
    24121 acggtggtta caggaagtta ttcattttaa tgtgctggcc attgtgcctc gtgattcaac
    24181 cgtcgagaca tttgcgttgg cggtcaatag tgcggcgatg gggatgatgt ttctgcctgg
    24241 agactggcga acgacgccgg aaaaggacat taaagatctc aaatcgttaa gcgcccgcca
    24301 gcgggagatt ttaaccatgt tagcggcggg cgaatcaaat aaagagatcg gcagggcgct
    24361 gaatatcagt accggaacgg taaaagcgca tctggaatca ctgtatcgcc gtctggaagt
    24421 caaaaatcgc acccaggcgg caatgatgtt aaatatctcc tcctgaagag gataatttta
    24481 cagccagcag gaagttagcg gctggctgta aaaatattat ggttcgccgt acaaaccaat
    24541 taaacggcgt accacaccgt tagtccagcc aaacccatcc tgcaacggat actcgccacc
    24601 gccgccttcg cgaggaacac catcggcaat atggtatttc tcgatcagtt tgtgctgttc
    24661 cagatagaac tgattcaccg tcttcagcca gttgcgcgcg atttcatcac ccagaaggtc
    24721 atcgccgtac atcttaaatc cctgaattgc catccattgt aacggtgccc agccgttggg
    24781 tttatcccac tgttcaccgg tttcgtactc gcttgccaga atcccgccag gtgtcagtaa
    24841 ccggctgcgc acggcgtttg ccagacgatc ggcctgttca tggttcgcca taccgacata
    24901 gagcggcaca atggcggcag cggaaaacag cgccagttgc tcgcgtcgcc agtcgtaatc
    24961 gcggtagatg ccgttttcat catcccagag gtaacggttt accgcatcgc gacgggcact
    25021 ggccttctgg cggaacagtg cttctgtctc tttctcgcct ttcagcgccg agatgttggc
    25081 gatggcgctc tccagtttaa acaggaaggc attcagatcg atggggatga actgggtggt
    25141 acgaatgctc gccagacgac cagtatcacg cagccaacgg gaagagtaat cccaaccgga
    25201 ggccgccccc gcacgtaaat cgcggtacac ctcgttaggc gggcgaccag aatgtttcgc
    25261 ggtttcaacg tcctcaagcc aggattcgtc acgcggcgtg tcgcgatcgt cccagtaacg
    25321 gttgagcagc gagccgtccg gcatccgcac aacatggcga taggcctgat tagggatcaa
    25381 cgattctgca ccgtccatcc agaaggcata ttccattttc aggtggtcga gatagcggcg
    25441 cgcgccgcgt acaccatctt cttcaaacaa ctccaccatc agcgcaaaaa ccggtggttg
    25501 cgagcggctc aaataatagg tgcggttgcc gttggggatg tggccgtagt tttcgatcat
    25561 ccaggcgaag ttatcggcca tgcatttcag caaatcttcc cgaccacttt ccgccagccc
    25621 cagcatggtg aaataggaat cccaatagta ggtttcgcta aaacggccgc ccgggacaat
    25681 atatgactgc ggcagcgcca gcagagaaga ccacggaatg tgatcctgtg gttcacgcgt
    25741 tagcaccggc cacagctggt cgatatgctc tttcagggaa ttttgcgggt ccgatacata
    25801 ctcgctggag tagacctccg gcagccagaa gtggttttca acaaacttgc gcaagtcaaa
    25861 atcacgatga cggcgcactt tacggtagcg gattaaaata tccagcgggt ccattttggg
    25921 tgcgcagtcg ggaaaggttt tactgtcggg gaaaattttc gccgactgaa catgctcgaa
    25981 cagttcgaga tagcgatcgg caggcgtcag cgcatcagag gcgggcagcc cctcaatcat
    26041 ctcgggttcc ggttctgcct cgatcatctc atccagtttt aattcatacg ggtccagctc
    26101 atagcagaga tcgacctcga tcatcagttc gtctggatta gggttttgaa ttttctgatt
    26161 gagcataagg atccgacctc cgaaattcgt cgctaattat cgggcttttc tttaagggta
    26221 gtcattcgct ccgcctggtg ggcgacaaag cgtgcggtgg atcacgttta ttaacctgcc
    26281 aaaaaatata acgcactaat atcaatggaa tttataaaaa tacggatttc gtgttgtttt
    26341 tcattttcag aacattcaat cctgctctcg ccgcaatcat gatatgccat tcctgatctg
    26401 ggtaataggc actggctatc ttattgatag tttatattca tgtaattgat tgattcctat
    26461 caaaaagcat atctccctgt tttttatgat ggctttgtct cgcagagacg tttgcagcat
    26521 tctggaaaat tttctgggtc acaataactc accaccgtga gggaagaata atgaaaatgg
    26581 tctcacgtat taccgcgatc ggcctggctg gcgtcgcgat ttgctattta gggttatctg
    26641 gttatgtgtg gtaccacgat aataaacgca gtaaacaggc cgatgttcag gcatctgctg
    26701 tcagtgaaaa taataaggtt ttaggctttc tccgtgaaaa aggatgcgac tattgccata
    26761 caccttcggc agaattaccc gcctattatt atattcccgg cgcgaaacag ttgatggatt
    26821 acgacataaa gcttggatat aaatctttta acctcgaggc cgtgcgtgcg gctctgctgg
    26881 ctaataaacc cgtttcgcaa agcgatctga ataagattga atgggtgatg cagtatgaaa
    26941 ctatgccgcc aacgcgttat accgcgctac actgggcggg taaggtgagt gatgaagagc
    27001 gggcggaaat actggcctgg attgcaaaac agcgcgcgga atattacgcc agcaatgata
    27061 ctgctccgga gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg cctaccgatg
    27121 cgcaaaaagt ggcgttgggt tttgcgctgt atcacgatcc ccgtttatcg gctgatagca
    27181 ccatttcatg cgctcattgc catgcgttga atgcgggggg cgtcgatggc agaaaaacat
    27241 cgattggtgt tggtggcgca gttgggccga ttaacgcgcc gacggtattt aactcagtat
    27301 ttaacgttga gcagttctgg gatggtcgtg cggcaacatt gcaggatcag gctggtggac
    27361 cgccgttgaa cccgattgaa atggcgtcga aatcctggga cgaaattatt gctaagctgg
    27421 aaaaagatcc gcagcttaaa gcgcagttcc tcgaagtcta tccgcaaggt ttcagtggcg
    27481 aaaatattac tgatgccatt gctgaatttg agaaaacatt aattacgccg gattccccat
    27541 ttgataaatg gttgcgtgga gatgagaatg ctctgacggc gcaacagaaa aaaggctatc
    27601 aattatttaa agataataaa tgtgcaactt gtcatggtgg tattattctc ggcggacgtt
    27661 cctttgaacc gttggggctg aaaaaagact ttaactttgg tgaaattacg gcggcggata
    27721 ttggtcgtat gaatgttact aaagaagagc gtgataaatt gcgtcagaaa gtacccggtt
    27781 tacgtaacgt tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg acgctggacg
    27841 gggcggtgaa actgatgctg cgctatcagg taggcaaaga gctgccgcag gaagatgtgg
    27901 atgatatcgt agctttcctg cacagtctga acggggttta cacgccgtat atgcaggata
    27961 aacaataatt aatttgatcg cccgaacagg agtgtttggg cgatttttat tacgat
//
LOCUS       DAZKHK010000068        28002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKHK010000068 DAZKHK010000000
VERSION     DAZKHK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.068"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            27..929
                     /gene="ybdO"
                     /locus_tag="ACHOS5_002847"
     CDS             27..929
                     /gene="ybdO"
                     /locus_tag="ACHOS5_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9777638.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FMNRKVSFRSSSLMTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            1076..2296
                     /gene="ybdN"
                     /locus_tag="ACHOS5_002848"
     CDS             1076..2296
                     /gene="ybdN"
                     /locus_tag="ACHOS5_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9777639.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESFNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLDSMPEKTAEHYRNKIAIYLHW
                     YQKKGVEVPQTQHGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2269..2898
                     /gene="ybdM"
                     /locus_tag="ACHOS5_002849"
     CDS             2269..2898
                     /gene="ybdM"
                     /locus_tag="ACHOS5_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9777640.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(2899..4059)
                     /gene="ybdL"
                     /locus_tag="ACHOS5_002850"
     CDS             complement(2899..4059)
                     /gene="ybdL"
                     /locus_tag="ACHOS5_002850"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP9777641.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFSALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLHAEPEHYLALPDFYRQKRDILVNALSESRLEILACEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4168..5256
                     /gene="hcxA"
                     /locus_tag="ACHOS5_002851"
     CDS             4168..5256
                     /gene="hcxA"
                     /locus_tag="ACHOS5_002851"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP9777642.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5266..5463)
                     /gene="ybdD"
                     /locus_tag="ACHOS5_002852"
     CDS             complement(5266..5463)
                     /gene="ybdD"
                     /locus_tag="ACHOS5_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9777643.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5576..7681)
                     /gene="cstA"
                     /locus_tag="ACHOS5_002853"
     CDS             complement(5576..7681)
                     /gene="cstA"
                     /locus_tag="ACHOS5_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP9777644.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7862..8275)
                     /gene="entH"
                     /locus_tag="ACHOS5_002854"
     CDS             complement(7862..8275)
                     /gene="entH"
                     /locus_tag="ACHOS5_002854"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP9777645.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8278..9024)
                     /gene="entA"
                     /locus_tag="ACHOS5_002855"
     CDS             complement(8278..9024)
                     /gene="entA"
                     /locus_tag="ACHOS5_002855"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP9777646.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9024..9881)
                     /gene="entB"
                     /locus_tag="ACHOS5_002856"
     CDS             complement(9024..9881)
                     /gene="entB"
                     /locus_tag="ACHOS5_002856"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP9777647.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9895..11505)
                     /gene="entE"
                     /locus_tag="ACHOS5_002857"
     CDS             complement(9895..11505)
                     /gene="entE"
                     /locus_tag="ACHOS5_002857"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP9777648.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIVVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(11515..12690)
                     /gene="entC"
                     /locus_tag="ACHOS5_002858"
     CDS             complement(11515..12690)
                     /gene="entC"
                     /locus_tag="ACHOS5_002858"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP9777649.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12879..13835
                     /gene="fepB"
                     /locus_tag="ACHOS5_002859"
     CDS             12879..13835
                     /gene="fepB"
                     /locus_tag="ACHOS5_002859"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9777650.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(13839..15089)
                     /gene="entS"
                     /locus_tag="ACHOS5_002860"
     CDS             complement(13839..15089)
                     /gene="entS"
                     /locus_tag="ACHOS5_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP9777651.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15200..16204
                     /gene="fepD"
                     /locus_tag="ACHOS5_002861"
     CDS             15200..16204
                     /gene="fepD"
                     /locus_tag="ACHOS5_002861"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP9777652.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLTTPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16201..17193
                     /gene="fepG"
                     /locus_tag="ACHOS5_002862"
     CDS             16201..17193
                     /gene="fepG"
                     /locus_tag="ACHOS5_002862"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP9777653.1"
                     /translation="MIYVSRRLIITCLLLLIACVMASVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17190..18005
                     /gene="fepC"
                     /locus_tag="ACHOS5_002863"
     CDS             17190..18005
                     /gene="fepC"
                     /locus_tag="ACHOS5_002863"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9777654.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTAKI"
     gene            complement(18002..19135)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOS5_002864"
     CDS             complement(18002..19135)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOS5_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP9777655.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLDEYLRSSPYVMDQLKEAKIDPLDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKTNINDVNFTPFKYQLRPSLPVKKD
                     GQGKAIIVILSALVGGMVACGGVLLRHAMASRKQDAMMADHLV"
     gene            complement(19382..23263)
                     /gene="entF"
                     /locus_tag="ACHOS5_002865"
     CDS             complement(19382..23263)
                     /gene="entF"
                     /locus_tag="ACHOS5_002865"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP9777656.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTNGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPSLLCGDVDIMLPDEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADTRYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDIPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPSERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMTPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFKEIGPLINTQINN"
     gene            complement(23260..23478)
                     /gene="ybdZ"
                     /locus_tag="ACHOS5_002866"
     CDS             complement(23260..23478)
                     /gene="ybdZ"
                     /locus_tag="ACHOS5_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP9777657.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23481..24683)
                     /gene="fes"
                     /locus_tag="ACHOS5_002867"
     CDS             complement(23481..24683)
                     /gene="fes"
                     /locus_tag="ACHOS5_002867"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP9777658.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLNTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24926..27166
                     /gene="fepA"
                     /locus_tag="ACHOS5_002868"
     CDS             24926..27166
                     /gene="fepA"
                     /locus_tag="ACHOS5_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP9777659.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            27341..27961
                     /gene="entD"
                     /locus_tag="ACHOS5_002869"
     CDS             27341..27961
                     /gene="entD"
                     /locus_tag="ACHOS5_002869"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP9777660.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTARELTDNIITPAEHKRLADCGLAFPLALTLAFSAKESAFKASEI
                     QTDADFLDYQIISRNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT         6764 a         7738 c         7175 g         6325 t
ORIGIN      
        1 gaagagacaa tacaacggag tatgttatgg ctaatcttta tgatctaaaa aaatttgacc
       61 tcaatttact ggtcatattt gaatgtattt atcaacatct cagcattagt aaagcggcag
      121 aatcattata tataacgccc tctgctgtta gtcagtcgtt acaacgttta cgtgcgcaat
      181 ttaatgaccc gttattcatt cgctcaggga aaggtatcgc gccgactaca acagggttaa
      241 acctgcatca ccatcttgag aaaaatctca gaggccttga acaaacgatc aatatcgtta
      301 ataagtctga actaaagaaa aattttatta tctacggtcc tcagcttatt tcttgtacta
      361 ataacaacat gctaatccgc tgtcttagac aagatacttc aatcgatata gaatgccatg
      421 acatactgct ttcagctgaa aatgccgaag aactgttggt tcaacgcaaa actgacctgg
      481 caattacgct gcaaccagta attagtcgct ctgtcatctg catgccgtta cacactattc
      541 gcaattccct tatatgtagc aacaaacatc ctcgtattac tgataccagt aactatgaac
      601 aaattatcgc cgaagaattc accctattga tatctaaatc agcagggatt gacgaaattc
      661 aaatggacat agatgaacgt tttatgaacc gtaaagtttc atttcgcagt tcttctttga
      721 tgactataat taatagtatt tctgttactg acttattagg cattgtgccc accgaactgt
      781 acgatttaca tcgtgatttt ttgaagttaa aagaaataaa actggaacaa cctcttcctg
      841 cggttaaact ttatatttcc tataacaagg cttcgttgaa caaccttgtc ttctcccgat
      901 tcatagatcg tttaaatgat agcttctgat tcattaccac tgacaaataa tatattacgc
      961 atcaattaat tcattgtgtg aatataaaat aatgtgctct atcttgcttc gtttatccta
     1021 taattagcgg attcatctca cgaatcaccc cgtaacgttc agagagcgtt tttagatgtc
     1081 tatttataaa atcccgcttc cactgaatat tctggaagct gcaaaagaac gtattacctg
     1141 gacacttaat accctacccc gtatctgtgt ttctttttcg ggtggtaaag attccggatt
     1201 aatgctgcat ctgaccgccg aaattgcccg acaaatgggc aaaaaaatct gcgttttgtt
     1261 tatcgactgg gaagcgcagt tctcatgcac tattaactat gttcagtcat tacgtgagtt
     1321 ttacgccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc
     1381 cctttctcaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca
     1441 gcccccgcaa gatgcgataa ccgatcctga ctttttctcc ttttaccagc ctggcatgac
     1501 cttcgaacaa tttgtgcgtg agtttgctga atggttttca caaaaacgtc cggcggcgat
     1561 gatgatcggc atccgtgcag atgagtcctt caaccgtttt gtcgccatcg ccagtttaaa
     1621 taaacaacgt tttgccgatg ataagccctg gactactgcc gcaccaggcg gtcatagctg
     1681 gtacatttac cccatctacg actggaaagt ggctgatatc tggacctggt atgctaacca
     1741 tcaacagctc tgtaacccac tgtataatat aatgtatcag gcaggcgtcc ctctgcgtca
     1801 tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt
     1861 tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg
     1921 catttacgcc ggacatgaca atcattttta tggtcatcgg aaaatcctca aaccagagca
     1981 tttagactgg caagagtatg cattattgct gcttgatagc atgccggaga aaaccgctga
     2041 gcattaccgc aacaaaattg ccatttactt gcattggtat cagaaaaaag gtgtcgaggt
     2101 gccacagacc cagcacgggg acattggcgc gaaagatatc ccctcctggc ggcgaatatg
     2161 caaagtttta ctcaataacg attactggtg ccgggcatta tcatttagtc ctacgaaagc
     2221 gaagaactat cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact
     2281 atgcaacaac gattaacgca ggatttaacc caatttctcg ccagcttgcc ggaagatgat
     2341 cgcatcaaag cgattaatga aatccgcatg gcgatccatc aggttagccc ctttcgtgaa
     2401 gagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac
     2461 ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgaaaa aatccattga aatagatggt
     2521 tttacccaac ccattgtggt cacgcatacg gataaaaatg ccctggaaat cgtggatggt
     2581 tttcaccgcc atgaaattgg taaaggctca tcatcgttaa aattacgctt aaaaggttat
     2641 ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat
     2701 aatcgcgctc gtggacgtca ccaaatcact gccatgtcag aaatcgtccg agaactcagc
     2761 cagttgggat gggacgataa taaaattggc aaagagctgg gaatggacag cgacgaagtg
     2821 ttgcgcctga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca attttcccgc
     2881 gcctggacgg ttaaataact acagctggcg caggcgttct gctgctgcca gcaacgtcga
     2941 ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac
     3001 cgacagcgga atcgccgcta cgccgtgctc ccgcgtcagc cactggcaaa actcaacatc
     3061 atccagcgta gatacggcgc tgtaatccac cagcaaaaag taagtacctt cgcacgctaa
     3121 gatctccagc cgactctcac ttaaggcatt caccagaata tcgcgcttct ggcgataaaa
     3181 gtccggtaac gcaagatagt gctcaggttc tgcatgtagc atatcggcaa tcgccagttg
     3241 cgccggggta ttcaccgaaa aggtcagata ctgatgcact ttgcggatct cggcgctgat
     3301 gggcgcaggt gcaacgcaat agcccacttt ccagccggtc atatgatagg tcttgccaaa
     3361 cgatgacacc gccaccgctc gctcacgcaa ttgcggatgc gccagcacgc tggcatggcc
     3421 ctgttgtgaa aaattgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc
     3481 ggcgatcgcc tgccacagag cggcgaaatc agtctgctgc cagacagttg cactggggtt
     3541 atgcggggtg ttgaggatca ccagtcgggt gcgctcgctt aacaatgcgg aaaattcctg
     3601 ccagtcaacg cgaaaatgcg gcggttgcag tgcaatacgt ttcactattc cccccgaaag
     3661 tgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt cgtcgccatt
     3721 gcgcaccaat gcggttatcg ccgcgtataa cgcctccgtc gcccctgccg tcacggtgat
     3781 attgctgtcg acatccggtt gatagccata cagacgttcc gttttctgag caatcgcctc
     3841 gcgcaaggcc tgcacgccgg tcatgggtgc gtattggttt gccccctggg caacgtggta
     3901 cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt gcgacaggtt
     3961 aatcgcctgg tgttgctgcg ccagcgcgct catccgggtg aaaatagtgg tgccaagttg
     4021 tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac ctgacgaagt
     4081 tgcgtgttga aggcactata acacgatgct agtatttggc aatcaagacg tttagatgtc
     4141 taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt ggtcgtcggc
     4201 ccagctaact acttttcaca tccaggaagt ttcaatcacc tgcacgattt tttcactgat
     4261 gaacaacttt ctcgcgcggt gtggatctac ggtgaacgcg ccattgctgc ggcgcaaaca
     4321 aaacttccgc cagcgtttga actgccaggg gcaaagcata ttttgtttcg cggccattgc
     4381 agcgaaagcg atgtacaaca actggcggct gagtccggtg acgatcgcag cgtggtgatt
     4441 ggcgtcggcg gcggcgcact actcgacacc gcgaaagccc tcgcccgccg tctcggtctg
     4501 ccgtttgttg ccgttccgac gatcgccgct acctgcgccg cctggacacc gctctccgtc
     4561 tggtataacg atgccggaca agcgctgcat tatgagattt tcgacgacgc caattttatg
     4621 gtgctggtgg aaccggagat tatcctcaat gcgccacaag aatatctact ggcggggatc
     4681 ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc agaaacgttg
     4741 ccgctaaccg tgcggctggg aatcaataat gcgcaggcca ttcgcgacgt cttgttaaac
     4801 agtagcgaac aggcgcttgc cgatcagcaa aatcagcaat taacgcaatc attttgcgat
     4861 gtggtggatg ccattattgc tggtggtggg atggtcggtg gtctgggtga tcgttttacg
     4921 cgtgtggcgg cggcccatgc cgtgcataac ggtctgaccg ttttgccgca aaccgagaag
     4981 tttctccacg gcaccaaagt cgcctacgga attctggtgc aaagcgcctt gctgggtcag
     5041 gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc gactacgctg
     5101 gcggagctgg aagtggatat caataatcag gcggagatcg acaaagtgat tgcccacacc
     5161 ttacgtccgg tggaatccat tcattacctg ccggtcacgc tgacaccaga tacgttgcgt
     5221 gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgta catatttagc agcagcgcgc
     5281 gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct cgtaggtcat
     5341 cggcgtttga tcgggatggt taacccgcat atgttcgaca tagttgtcgt aatcaggcat
     5401 accaatcatc agtttcgccg cctgacctaa atattttccg gcttttgcca gtgaatcaaa
     5461 catctaaacc tccggtaata caaatgccct cctgaattaa gagggcttaa ttgctatcaa
     5521 cacgctcggg ctgagagtaa ggggaacgct cagcacatcc cccctcactc tgactttagt
     5581 gcgcgccttt cgcctgcgcc acgatctcct cgacattttc cggcattggc tcatacggcg
     5641 tttctttcgc cgttggcttc ggctctttca atgccgccag tgccgtctta atcgagaaca
     5701 gcgccagaac caccacgacc accataaaga agatggttaa cccggcatcc agacggttgt
     5761 tgaataccag ttgcgccagt tgtgactcag tatactgcga cggaatattg ccgctgtcga
     5821 tcattgcctg gaacttgtta gcaatcgcca ggaagccgac ttttgcatcc gggctaaacg
     5881 ctttctgcca gccagcggtc agggtacaaa tcagcagcca ggccgttggt accagcgcca
     5941 cccaggcgta acgttgacgt ttcattttga acagcaccac agcacagagc atcagcgcca
     6001 tccctgccag catctggttg gcaataccaa acagcggcca caaggtgtta ataccgccca
     6061 gcggatcgac cacgccctga tgcaggaagt agccccacgc cagcacgcac agcgccgttg
     6121 ccagcaggtt agcaggcagt gaatcggtcc gtttcaggcc cggagacacc acgcccagca
     6181 gatcctgcaa cataaagcgc gcagcacgcg tacccgcatc caccgccgta agaataaaca
     6241 gtgcttcaaa cagaatggcg aagtgatacc agaacgccac atccatcatg ccgcccagcg
     6301 caccgtgcag aatgtaggcc atccccaccg ccagggtcgg cgcaccgccc gcacgggaaa
     6361 tgatcgactg ttcacccact tcgctggcaa tctggtttag cgtatccggg gtaatcgcaa
     6421 agccccagct actcaccacc tgcgcggcag aagcaaccac atccgccgtc cctgccggag
     6481 ccagcacagc catcgggctg ttcatggcga agtacacacc cggatcgatg atacaggcgg
     6541 aaaccagcgc cataatcgcc acgaaggatt ccattaacat cccaccgtaa ccgataaagc
     6601 acgcctgccc ttcgttcgcc agcatcttcg gtgtagtgcc ggaagagatc agtgcatgga
     6661 agccagacac cgcgccacag gcgatggtga taaacaggaa cgggaacagg ttaccggtcc
     6721 ataccgggcc agtgccatcg acaaatttag ttagcgcagg catggtcagc gtcgggcgca
     6781 taatcaaaat gcccaccgcc agaccgacga tagtcccgat tttcaggaag gttgagaggt
     6841 agtcacgcgg agccagcagc aaccacaccg gcagcaccgc cgccacaaaa ccgtaaccca
     6901 ccagcatcca ggtcagctgc acgccggtaa agtcaaagta cggtgcccag gtcggacttt
     6961 ctgccaccca gccgccagag ataatggcga aaatcaggaa caccaggccg atgaccgaca
     7021 cttcgccaat acgccccgga cgcagatagc gcaggtagat ccccataaac agcgccagcg
     7081 gaatggtgaa cgcaacggta tacgttcccc acgggctatg agtcagggct ttaaccacga
     7141 tcatcgccag cacggcaagg ataatgacca tgatcataaa gcaggccacc agcgctatca
     7201 ccccggcggt tggccccatc tcttctttga ccagctcacc cagcgagcga ccgtcacggc
     7261 gcgtagaaac aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc accccggcga
     7321 gcagccagat catccctggc aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg
     7381 gacctgctcc ggcaatggcc gcaaaatggt gaccgaacag cacttttttg tccgtcggca
     7441 catagtccag cccgtcgtta tgacgcaccg ctggcgtcat acgcgtcggg tcaaccgcca
     7501 gcacattttt ggcgatatac agcccataaa aacggtaagc gatcagatag atacagaccg
     7561 acgccaccac aatccacagc gcgttgatct gttccccacg atttaaagca atgtatccca
     7621 gagcaaatgc tcccattaca gagagcactg tccagacgag gtatttccct gatttgttca
     7681 tagttgttat ccgtgtgcgt gtccatagag atgttacatt ttgtttctat aacatctttg
     7741 tacaatttaa caacacagca accatgtaaa tcggagttga aaccaatatt taacttaaca
     7801 atgttaactc gatcactccg ttaaccgaat tttcctgcaa tctcatttta ttctgtcggg
     7861 ttcatcccaa aaccgctgtg cccagccgac aggtgcagca acgccgcccc tgttcatcga
     7921 aaacgacgat ttcccagctc tgattttgtc gtccaagatg cagcggttgg cagacaccgc
     7981 gcacctttcc ctcagacacc gggcgatgat gtgtggcgtt aagttccgtg cccaccacac
     8041 actgtccgtc gcgggtcatc ataaatccgg ccatcgatcc cagcgtttcc gccagcgccg
     8101 ccgacgcgcc gccatgcagc aggccaaacg gttgatgagt acgggtatca accggcattt
     8161 cggcttccag cacatcatcg cccagacggg tatacacaat tcccagatgc gccaccattg
     8221 tgttatcgct ggtggcgttc agctcgtcga gcgttaaatg gcgtttccag atcatgctta
     8281 tgcccccagc gttgagccgc catcgaccac aatatcctgt agggtgatat ggctggcgag
     8341 atcagaggcg aggaacaaaa tcgtattggc aatctcctgc ggacgggcga ttttccctag
     8401 cggaatgccg agtttaaact gctcgccaaa gccgcgaata cgctgctctt cggcgtcatc
     8461 gctcacccac agcgtgcgtt gcatatcggt gtcggtggag cccggcgaaa ccacattaca
     8521 gcgcacgccg ctacccgcca gttccagccc gacgctcaac gccaggcttt tcagcgccgc
     8581 tttcgacgcg ccataagcac tcataccaat gcgcggcgtg tgtgcggcgt cggacgcaac
     8641 agtaacaatc gccccgcccc gctgacggcg aaactggttc atggtctgct gaaacaggtt
     8701 aaacgcaccg ccgacgttga ccgcaaaagt ctgctgccag tcctctttac tgagctgatc
     8761 ggtcgcgccc atgcgcaaaa ttcccgccgc attgaccagc acgtccagtc gctccgtttc
     8821 agctaacagt cgctgacata cctgcgccac ctgcgcagca tcggcaacat ccattacttc
     8881 ggtcgcaaaa ggatattgct cctgagtaaa cgcttgatca aaacctgtaa ctttcgcccc
     8941 cgcctcaaca aatgccagcg ccgtggcata gccgatacct ttccccgcgc cagttaccca
     9001 gacattttta ccgctgaaat ccattatttc acctcgcggg agagtagctt ccaccaggcg
     9061 tcgatggtcg ggtttttcgc cagcatgaca aagtcgatat cgccatgcac tttgcgccag
     9121 cgcgccgcca gcgccatcat ccgcaccgaa tccagaccgt agtcgatcag gttgtcgtca
     9181 tcgaacggtt catcggactc gtccagcaac ggcaggataa cctcacgcag cgccgctttg
     9241 ctggcgggga caggtgctgg cagtaattct tcagtcatca ccacccggcc agaccgtccg
     9301 gccacatatt tcagcgacat caaatgctcg tcacggctga aatcggccag cgcatccgcc
     9361 accataaacg gtttaatatc gcgcataaat gcgtccgttg cggtggtcat acagccaatg
     9421 tgggcatata ccccggtaat aatcagctgg ttacgtccac tctctttgag catttgctcc
     9481 agcggggaac gatgaaacgc gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct
     9541 ggcgtcaggc gatccaccac cttttgctgt tccggcgaac gggtcaggcc tggcccccac
     9601 atatcattca acagcgcccg atcttcatcg ctctgctctt tcggctgggc ggtgtaataa
     9661 accgggatat tgtgctgttt gcagtagtcg cgtagcgcag caatattcgc aatcacctgc
     9721 tccatcatcg ggcagttctc accccagaag ctgacaaaat agtcctgcat atcatggatt
     9781 aacaacgcgg cacgttgcgg ttcaaaggcc cagtcgactt tattctgcgg aatatcatga
     9841 ggctccggca gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc
     9901 tgatgtgcgt gacgccagcc actgacgtaa ttgtttttta tcgactttcc cgaccgccgt
     9961 cagcggaagt gaatctacac actccacgcg atccggtaat ttaaattcgg caataccctg
    10021 ttcacgcagg aaacgacgca cctgcaccgc gcgcagcggc tcttttacca ccagataagc
    10081 acagcttttt tcgcccatca gctcatcttc catgctcacc agtgcggcgt agatcaccgc
    10141 cggatggcgc agcagcaggt tttcgatctc ttcggcagcg atcttctcgc cgccacggtt
    10201 aatctgatct ttctcgcgcc cctgcacagt gatgtaaccc tctggatcaa tagagatcag
    10261 atcgccggaa cagtaaaaac cgttggcatc aaaggcgctg gcattgtgct gcgggctttt
    10321 gtaatagcca cggaaggtgt acggcccgcg cgtcatcagg cgtccaactt ccccttgcgg
    10381 cagtggattt ccttcggcat cggcaaccca tacttcatcg tccgggcaca ttgggtaacc
    10441 ctgggtatgg ataattttct ccgcactatc atcaagacgg gtgtagttca ccagcccttc
    10501 cgccatgcca aacacctgct gcaactgaca gccaatctca gcgggaatac gcgccgcaag
    10561 ggtggcagaa agacgggcgc cgccgacctg taacagtttc agcgaggcaa gctgcgcccg
    10621 gctttcgcct tcagccagtg cctgcaacca caggctgact gctggcggca ccagcgcggt
    10681 gacgttcacc tgatgttttt caatcaatgg gaagcaaagc gtggcgctgg ggtcggcagc
    10741 cagaacgaca gtgccgccag cgagaaatac ccccaacgat cccggtgaac tcatggcgta
    10801 gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaact gacaaatctc
    10861 gacgctacga cgcacgctgt agtagtagtc gttatgagtg cgcgggatca gtttcggtgt
    10921 gccggtggtg ccgccggaaa gctgaaaata ggccacttca tcggcaggtg acggcgtggc
    10981 agtaaaatcc tcagccggat ggttaatcgc atcctgcaag ttatgctcgc cgctgtcgtt
    11041 gagcagctgc accacgcgaa tggaagaatg ctctgcaaca aatgtgttga ggaaatcatc
    11101 cccgctaaat agcgcatgtt ggcgatcggc aatcagtaac gccggttcaa tctggctggc
    11161 ataggcgttc agttcactac gctgatggct gaacagcgcc agcaccggcg caacgcccag
    11221 tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg ttacccagtt gtaccagcgc
    11281 ggtttcacca ggtttaatgc cctgacggcg caaactacag gcgaggttat ccgccgcctg
    11341 attcagctcc cgataactca actgccgctc gccgtcgata accacgatgc tgtcactcgc
    11401 agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt tttcccgata
    11461 gcgacgggca aactcttccg gccagcgggt gaatggaatg ctcatcctcg ctccttaatg
    11521 caatccaaaa acgttcaaca tggtagaaag tttgacgccc gtttcgcgcc actcacccaa
    11581 cggggaggac gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat tttcccgcag
    11641 cttcgcgcag cggatggtca ccacccattc accgttacct tcgctgtcac accaacccac
    11701 aatgccgcca aacagttcgc ggtcgaacgg ctccagttca gcaataacct gagtcgcggc
    11761 ctgatgcggg aaaccgctca gcgcgggggt cggatgcagc agacaagcca aagtcagtgc
    11821 gttttcttgc gaattcgctt taccttcaaa gggagttgcg agatgccaca gcgtcggcgt
    11881 ggtaatcaat tgtggagagg agggaacgtg taactcacta ctgcgtttgc gcagtacctc
    11941 tttcatcgcc tgagtcacca gttcatgttc atggcgatct ttttctgacg ccagcagacg
    12001 attacccgct tcgcgatcga gcacttcatc cggctgacga cgcgcggaac cggctaacgg
    12061 aatggagcta aaacgctcgc cgtctttacg tagcagcagt tccgggctgg cccccagcag
    12121 aacgccacca tcagccaacg gaacatggaa gttgtaacta accgggtttt gcgcaatcaa
    12181 ccgttccagc aatacgccac tatcaatggc ggcgtcagta gtgatatcaa tcaaccgtga
    12241 caacaccact ttgtcgacct gcggcgtggc ggtaagtgcg gcagcacggg caaccatctg
    12301 ttcaaacgtg gtttgttccg gaatggcctg acgttccacc acgttcagcg actggctgcg
    12361 ggtgaaacgg cgtgcggaag cttgtttttc ctgacgggag aacgactgcc aggattcggg
    12421 aatatacagc gacgaaggct gacgtggatc gaagggaatc gccccgacca tcaccggatt
    12481 tttgatgccc tgcgctttgg catcggcaaa caacgcggcg agtttttgct ggaagggact
    12541 gtcgggcgaa tccccgttca cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt
    12601 aaaactgcgg tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg
    12661 ctgtacttcc tcagccagtg acgtatccat atcatcctcc acaaaatgat aaaggcttta
    12721 ataatgatta tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa
    12781 ctcttgaggt aacgcaatct gcaactcggg gttgcatctg gttgtggtta caatgaaaat
    12841 gagaagcatt aacttcatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    12901 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    12961 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    13021 gtattgtttc caccagcgtc accctgactg gctcactgct ggcgattgat gctccggtga
    13081 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcgga tgaccagggc tttttacgcc
    13141 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagtg
    13201 cagaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggcgatt
    13261 cggcgctggc gctgtacgat cagctttcta ccatcgcccc aacattaatc atcaattacg
    13321 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    13381 aagcggcaga gcggattgca cagtttgata agcaactggc ggcggcgaaa gagcaaatca
    13441 aattaccgcc gcagccggtc actgccatcg tctataccgc cgctgcacac agtgccaatc
    13501 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    13561 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    13621 ttggtggaga gaatctggct gcagggttaa atggcgagtc actattcctg ttcgccggtg
    13681 atcagaaaga cgccgatgct atttatgcta atccgctgct cgcgcacctg cctgcagtac
    13741 aaaacaagca ggtttatgcg ctgggaaccg agacgttccg cctggattac tacagcgcca
    13801 tgcaagtgct ggatagactt aaggcgctgt tttaagcatt aactgtcgga cgctgtcacc
    13861 tgcggcggcg tctggcgaaa acgtcgcaac tccactagta ccagcagcaa caacacgccg
    13921 ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat catcgcgccc
    13981 aaaccgccca gcagcgccgc gcctatggca tcgcccgtca cgttttgtgc cgtccacaaa
    14041 ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt atattgcagc
    14101 aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc taaaatccac
    14161 atcggcatca ggccaaacaa accaatggcg aggaacgatc ccagcgtgga aagcagcatc
    14221 aataaccctg gtcgcgcgct atgtgccagc ttaccgctgg ttaacgcgcc aatagccgcg
    14281 ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg ccagttgtca
    14341 gccagcgccg gatacaggac ccgcaccgcg ctcgccatcg tcaataaacc ccccagcaac
    14401 gcaatccctc ccaccagcgg gctggcgagt agaaaacgaa atcctgccag taatgatttc
    14461 aacggatgct cacgcggctg cggcggtggt ggcaacgccg gaaggcttaa caacggtagc
    14521 aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggctacgcc gccggtcgcc
    14581 agcagtaaac cgccaatcat gggcgaaatc accgatccca gacgcacagt caacatggtg
    14641 atcgccccgg cctgcattag gttttcacgc cctaccagtg caggtgtcgc cgccagtagc
    14701 gccgtaacgc caagtgatgc gaaaaaacca tcccataaac caagtaaata gattgccagc
    14761 aaagacggct ccggcagcag cgcattcagg cacagtccaa tgaagccaat gccacaggtg
    14821 ccacgcgcca gcaaaatcac ttttttacgc tcatagcgat ccgccagcac accgccgacc
    14881 atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac ctgccaggta
    14941 gaatgtgtca tcatctggat ctgcaccggc accgcgacgc cgagcaaacc cagagacaca
    15001 attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt taacaggctg
    15061 aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat attttaaata
    15121 cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga taattaattt
    15181 cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc
    15241 cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg gagcaaaatc
    15301 actccccgct tccgtagtgc tggaggcgct ctccggcacc tgccagagcg ccgactgcac
    15361 catcgtgctc gacgcacgac tgccgcgcac ccttgccggt ttactggcag gcggtgcgct
    15421 tggccttgcc ggggcgttaa tgcaaaccct cacccgaaac ccacttgccg atcctggttt
    15481 gcttggcgtg aacgccggtg ccagttttgc cattgtgctg ggcgcggcgc tgtttggtta
    15541 ctcttccgcg caggagcaac tggcgatggc cttcgccggg gcgctggtgg cctcattgat
    15601 tgttgccttt accggcagtc agggcggcgg gcagttaagt ccggtgcgtt taacccttgc
    15661 aggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc
    15721 cgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc gcaatctaca
    15781 taccttaaaa gtggtgctga tcccggtgct taccgctgga gcaactgcgc tattactgag
    15841 ccgcgcgctg aacagtttaa gccttggcag tgacaccgcg acggcgctgg gcagtcgcgt
    15901 agcgcgcaca cagttgattg gtctgctggc aattaccgtg ctttgtggta gtgcgacggc
    15961 ggtagttggc ccgattgcct ttattggcct gatgatgccg cacatggcgc gctggctggt
    16021 aggtgccgat catcgctggt cgctgcccgt cacgctactc actacccctg ccctgctgct
    16081 gtttgccgat atcattgggc gggtgattgt tcccggcgaa ctgcgcgttt ctgtagtcag
    16141 tgcctttatt ggtgcaccag tgctgatctt ccttgtacga cgtaaaacgc gaggtggcgc
    16201 atgatttacg tctctcgtcg attaattatc acctgtctgc tgttgctaat agcctgtgtg
    16261 atggcaagcg tgtggggatt acgcagcggt gccgtcacgc tggaaacttc gcaggtattt
    16321 gccgcgctga tgggtgacgc accgcgcagt atgacgatgg tggtcaccga atggcgttta
    16381 ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg cgcgattttt
    16441 cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa caccggggcg
    16501 tggagcggcg tgctggtggc gatggtgctg tttggtcagg acctgacggc tatcgcgctg
    16561 gcagcaatgg tgggcggcat tgtcacttcg ctgttggtct ggctgctcgc ctggcgtaac
    16621 ggtatcgaca cctttcggtt gattattatc ggtatcggtg ttcgcgccat gctggtggcc
    16681 tttaatacct ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg
    16741 aatgccggat cgctcaatgg cctgacgtgg gcaaaaacct cgccttccgc acccatcatt
    16801 atattaatgt taattgccgc cgccttactg gtgcggcgaa tgcgcttgct ggaaatgggc
    16861 gatgataccg catgtgcgct gggcgtcagc gtcgaacgtt cgcgactgtt aatgatgctg
    16921 gtcgccgtag tgcttaccgc agctgccact gccctggcgg ggccaatttc ctttattgct
    16981 ttagtcgcgc cgcacattgc ccgacgcatt agtggcaccg ctcgctgggg gctaacccag
    17041 gcggcgctgt gcggggcgct gttactgctg gtggccgatc tctgcgccca acaactgttt
    17101 atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc
    17161 gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgcggcg
    17221 aacagttaac cctcggatat ggcaaatata ccgttgcgga aaatctgact gtagaaatac
    17281 ctgatggtca cttcacggca attatcgggc caaatggctg cggtaaatcc acgttactgc
    17341 gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat ggcgagcaca
    17401 ttcaacatta cgccagtaaa gaggttgcac gccggattgg tctgttggcg caaaatgcta
    17461 ccacaccggg cgatatcacc gtgcaggagc tggtggcgcg tgggcgttac ccgcatcaac
    17521 cgctgtttac ccgctggcgc aaagaagatg aagacgcggt aacaaaagcg atgcaggcca
    17581 cgggaataac tcatctggcg gatcaaagcg tggacaccct ttctggcgga caacgccagc
    17641 gagcgtggat cgcgatggtg ctggcccagg aaacggcaat tatgctgctc gatgaaccca
    17701 cgacctggct ggatatcagt catcagattg atttgctgga actgttaagc gaactgaacc
    17761 gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg
    17821 ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagca ccgaaggaga
    17881 ttgtcactgc tgaactgatc gagcgcattt atggtctgcg ctgcatgatt attgacgatc
    17941 cggttgccgg aacgccgctc gtggtgccgc tcggacgaac ggcaccttca accgcaaaga
    18001 tttaaactaa gtggtctgcc atcatggcat cctgttttct ggatgccatc gcatgacgca
    18061 acaacacacc accacaagcc accatcccgc cgaccaacgc agaaaggatc acgataatcg
    18121 ccttaccctg accgtctttt tttacgggta atgacggacg caactgatat ttaaacggtg
    18181 taaaattcac atcgttgata tttgtttttg ttaattgctc gactagatac tgacgattac
    18241 gtaattcacc gttcaattcc gcaacgtcag tgactgcttt ttcaatttcc agtttgcgtt
    18301 caataccgtc tgcaccgaga gaaatagaga aatcggggtc atctttaacg gcctgaccat
    18361 tactgtatac gggcttttta attcctgccg cgttggcaat gtcaagtgaa taattgaggc
    18421 gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc agtttttctt
    18481 tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac tctttcacca
    18541 ccaacgcaga gatataatcg atatacccgg ccaacacttt ctgtgcctct tcactggtgg
    18601 gtgcggtgaa acttagcgtc caggaagtat acaatgcaga ctcatctttt ttcttactgg
    18661 cattgtcatc aaccgctttc attttttcgc tcaatgcaac aatggcgcga tgcagatcca
    18721 gtgggtcgat tttcgcttct tttaattgat ccatcacata aggtgatgaa cgcaggtact
    18781 catcaagcaa gctaacagac tgaaatttct tgataaacag gttaaatgct tctgtacgat
    18841 caattttgat atccagatcc agcacacgaa gcttagtgaa tgttttctcc agctcttgcc
    18901 actgaacagg ttctggaggc gtgacaaccg ccgcgctagt ccatttttgc ggcaggatga
    18961 aagagatcag caagcctgcg caggcaaacg caaaaacgac cgccatgacc gttttttttg
    19021 cccgccataa aacctcgatt agattaagta aatcaatttc attattactg ggcgacgcca
    19081 gaggatactc gggaaaatga gcttcacttc cctgtttaat attcagtgat gacatgtagt
    19141 gaaacctgag gttttagtcc gtaaagcaca acgatgtaca tagcattcta acaggaactg
    19201 agatttatcc gaaagcactc tttcactatt attattctca aaatgaataa caaacttagc
    19261 aaccccagca atgacgctgc tctcagaaaa ataaataaat tttaagaata aaaaaaccat
    19321 aatcatttag tcggattatg gttttacatg aggttatggc aagttcttta ctaacgtacg
    19381 gttagttatt aatttgtgta tttattagtg gtcctatttc tttaaaatac tccggggaaa
    19441 taatatccac atgcgcacaa tcctgacgat agatatccag ctcagcaatc cacggtgccc
    19501 aggcctgttc aggagtcata ccttcctgaa gcgtgcgttc ggcaacaaat agcgtcgctt
    19561 taccatcaaa cggtacgcta tgagctgtcg tcagcaggcg cacagcatca gcgtagttgc
    19621 cttcaatggt ggtaaacaac tccgttgagg tacttccctg ctgtgccgcc aggaaggctt
    19681 cgcgctcgcg gttaatctcc gccagcactt ccggatccag accattggct tctttttcct
    19741 gccagttttg cgtttctggc ggccaggtat ccagcaagcc aagaaatgcc acctgttcgc
    19801 cacgggcacg cagtcgcgcc gcaatgccct gcgccagcgt accgccaagt gaataaccca
    19861 gcaggtaata tgggccgtgc ggttgttgtt caagtaaggt tgccagatgc gcttcgcaga
    19921 cttcatccag gtttgccgcc gtctgcatgg ggccatgagg gcgcggcgac tgaatgccga
    19981 taatcgacca ttgtggatcg agataacgcg agagcacgct gaactgccag gcaaaaccgg
    20041 atgccggatg gaaacaaaac agcgtcgggc cattaccttc acgcaacggc agaatggttt
    20101 cgaatcccat gcgctgggtg ctgtcttctt caccatcaat aatcgttgcc agtttggcga
    20161 cagttgacgc gaccatcacc tgccccggcg tcacctgacg agcaaactgt cgacttaact
    20221 gcgctgccag tttcattgcc agtagcgaat gaccgccaag cgcaaagaaa tcagcatcgg
    20281 catcctgcac gtcacaaccc agcaacgacg agaatgccgc ggcgataatc gtttcactgc
    20341 ccgctttcgg cacacgcccc ggcgtttgtg ccttcagttc aggcaacggt aaggctttgc
    20401 gatccagctt gccgttggcg ctaagtggta actgtggaag ttgcagcaga accaccggta
    20461 ccatatgtgg tggcaatgtt tctcgaagct gcgcctgtaa tgcgctggta tccaacggca
    20521 gacctgattg cgacaccaga tagcccacca actgacgcgc atcaccacca gtggctgccg
    20581 cctggttaat cacacaggcg tgggtaacgg cttgttcgac atccggcagc gcctgcatca
    20641 cgcgatcaat ttcgcccagt tcgatacgct gcccgcgaat ttttagctga tcgtcactgc
    20701 gcccgaggta ctccactgcg ccgttatcca gccagcgagc cacatcgccg gtacggtaca
    20761 tccgttcact tggggcaaaa ggatcggcaa taaagcggct ggcggtcaga tcgggtcgtc
    20821 caagataccc ctgcgccagt tgaataccgg tgagatagag atctcccgcc acacccggcg
    20881 gcaccggatg catcatcgca tcgagaatgc gcaggcccgt attccacacc ggataaccaa
    20941 tcggcacact gctgccgcgc acctctgcca gttcctcgcc aaaagccgga taccaactga
    21001 catctaccgc cgcttccgtc gggccatata gattatgcaa cggcgcgccg gttaactgtt
    21061 gccattcgcg gcataaatca gccggtaagg cttcaccact acagaaaacc tgtttcaacg
    21121 tcgcgcaact ctggcgagcg gtttgcggcg tcagcgatgc aacaaatgcc gccagcatcg
    21181 acggcacaaa gtgcgtggtc gttacgccat attcggcaaa gaattgctgc atagcgagcg
    21241 ggtcgcggtg cgcttccggt tcagccatca ccagttttgc cccggcaata aatggccaga
    21301 aaaactccca caccgagaca tcaaaactgc acggcgtttt ttgggcaacg acatcttcgc
    21361 ctgtaagcgg gtaatggttt tgcatccaaa gcaggcgatt aacgatagcc gtctgcccga
    21421 ccattacccc ttttggtctg ccggtggaac cggaggtgaa gatgatataa gccgtgtggt
    21481 gcggttgtga aagctgcagc ggcgcactgc cctgcggtgt aagcggggcg ttatagcaaa
    21541 ggctggttaa attagggata tcgctaaagc gcggcagttg atcgtcagtg gtgattaaca
    21601 gcgacggacg cgcatcttcc agcatcattt tcaggcgatc gtccggatag ccggtatcca
    21661 acggtaacca ggccgcacca gcttcaacta tcgcatgtag cgccagggtc aaaaagaccg
    21721 agcgcggtag cgccaccgcc acgctgtcgc ctggtttaac gccgcgctca cgcagcagat
    21781 tcgccagcgc caccacctgc tcgcgcattt cccgatagct gaactggtaa cgcgtatctg
    21841 ccagcgccgg agcatccggt gtttttgccg cttgttctgc caccagcgcg ctcagcgtgg
    21901 tttctggaat ctcaacctga gtggcgttga tttgcgccag ctgcgcatac tcatctggca
    21961 gcataatatc gacatcgccg cacaacagcg acggatccgc ggcgaactgc gcaatcagca
    22021 ttttcaggcg ttcagcgtgt tgaattaacg ttggctcatc gtaacgctgt ttattggcga
    22081 ggatctcaat actcaaatca ccgtgttcat ccgggaacag ggccagttca aggtcattaa
    22141 ccggaccggt tgccagggta tgggtttgcg cctgaacacc aggaatatcc agttggtaat
    22201 caaatacctt gatattgagt accggaccga acagcggttc ctctcctgcc gctcgcccgc
    22261 tgtcacggac aatttgttcg gcatcgtaac gttgatggcg acgcattttt ttcagttgag
    22321 ccgccagtcg ggttgccagt tctggtagcg tttcttgcgc cgcaatgtga atacctaatg
    22381 gcaaaacgtt gagcacgggt ccggtagccg tcagcgccgc cgagcccagt cgacgcataa
    22441 agataaatcc ggcagcgtag tccatgcggt tgcacaatcg ccccagccac aaggctgcca
    22501 acgcaagtgc taaatcggta cgctgcacac ctgaaagttg cgtagccagc tggcggaatt
    22561 ccccgttggt aaattccagt ttcaggcgca aaatatctgc cgaggcgctg cgccccggta
    22621 aaggtgccgg agaaagtgac gcgggcggcg gcaattgacg acgctgttcc gcccagaatg
    22681 ccgcatcacg ctgccaggct tcgctttcgc ggtattgctg gtactcttcc acgacatcag
    22741 cgaaaggcgt aaacggcgaa gcaggcgttg gttcgccacg cagccatgcg cagtaaatat
    22801 tggcgatctg gcgggtaatg gccgggaaac tgaagccatc gaccagcaaa tggtgataac
    22861 gctgatacca gtaccagcga ttatccgcca cctgtatcaa ctgatgaaag accagtggtt
    22921 taccgctatc gacgcgcaga tcttgttgca aatccgcctg cattaatgcc cgcgcagtac
    22981 cgtgcggatc aatattggtt cgcaggtcga taatttctgg cagttcgaac gtcagcgcat
    23041 catcgaccca ctgccagact tcgccgttat cttccgtaaa acgcatccgc agcgtatctg
    23101 cttgcgctaa tccggcaacc accgcgcggg ccagtagtgg cgcatcaacc tctccggtta
    23161 actcaacgta atgcgccacg ctccaggcgg agggtaaatc cgacagtttt tctgccatcc
    23221 agatgccggg ctgtgcggcg accaaaggta aatgctggct cattgtgcct cctgcaactg
    23281 ggtgaaattc gccggtgtca gagtacgcca gtgggcttcc agccactgct ggcaggacgc
    23341 ctgtgactgc ggctgacaca caacgtccca gcctgccggt aaggcgcatt gttgcggcca
    23401 cagactgaat tgcccctgcg cattgcgcaa aatgtaaaac gctccctgtg gatcatcgaa
    23461 gggattactg aatgccatat tcaactcctg tcatggaaaa gtggttgcca gaggtcgatt
    23521 agcccctgca tcaagccacc gcgccaacaa agcgcatcat gtccgccgtc aacctgacgc
    23581 cagaaaatgg attcttttag ggggtgtaat tgcgcataca gcgcctgatt ggcctgcatg
    23641 atcatcggct cgcgaacacc cgcttccagc acaatgcgca gaccttcggc gctaacttca
    23701 ccagtattta gctgttcaag taacatgccc tcttggtgcc cgccccgatg cggccaccag
    23761 tacgagcctg actggcttaa tacacagcca aagcgttcag gccagtgcag tccggcatac
    23821 agcgcggaaa gcccaccaaa actctgtccg gcgaccacgg tgcgatcggc gcgatcgcta
    23881 aaaggggcaa tagctttcac ctggggtaat aactcttgct gtactgccag ccagaaatcc
    23941 gcattacacg gcagttcgtg ggcgcggtgc gtggtgtcga tagcgtcgat caacacatac
    24001 acggcgggcg gaagttgccg acgatgggtc agcgaagtca gcgcaggcca gacgggcata
    24061 ctctgcgccc aaaattcgcc atcaagcaaa actgccagcg ggcgttcttc tgctgttgca
    24121 tcgccggtgg taaaaatcca tacacggcgt gaatttttca accgttcact tttccagata
    24181 atttctttgg caggcgtttc tggcgcttgc ggacaatccc atcccggttg cagaggcgct
    24241 tgcggcattt cgagtgcaga aacagcgtgc cctcgcccgc ctttccagct ttgtggattc
    24301 agcggatcgg ctatcgcctg gggtaatagt tttcgccagc cttcgcgtaa ttcgaggcga
    24361 tcgggggatg gtgcagaaaa aatgtcatcg cgttcagtag gaataaagca gtagctgccg
    24421 cgccagttgg cattgagttg tgtcgtccac tgccaaacat cggttcctgc aattcgctgc
    24481 atcgaccggg gctggctgtt ctgatggtga tcggtcacgc cagtgatata gacccatacg
    24541 cgctttatcg tcgagtattc ttcagaaccc tggggatcac gccaccagaa agtgacctca
    24601 aacatttcgt cattcagacg ttgccattcc gggccatgtt tcgactgcca ccagctctca
    24661 cttcctactt ttaacgccgt cacaccataa ccccatgttt actgtgcaat tttttattga
    24721 ttgcagaaat atattgataa tattattgat aactatttgc atttgcaata gcgtaatggc
    24781 gcgccgtggg aagcgcggac attaattaat cagctgcact gcgtgtcttt caggatcaaa
    24841 ggttttcgcg gtagcgggat gcgtcgtgtt gatggcgacc atgcccgaca gttgcaattc
    24901 gtggcaaaaa tgcaggaata aaacaatgaa caagaagatt cattccctgg ccttgttggt
    24961 caatctgggg atttatgggg tagcacaggc acaagagccg accgatactc ctgtttcaca
    25021 tgacgatacc attgtcgtta ccgccgccga gcagaacttg caggcgcctg gcgtttcgac
    25081 cattaccgca gatgaaatcc gcaaaaaccc ggttgcccgc gatgtatcgg agatcattcg
    25141 taccatgcct ggcgttaacc tgaccggtaa ttccaccagt ggtcagcgtg gtaataaccg
    25201 ccagattgat attcgcggca tgggtccgga aaacacgctg attttgattg acggcaagcc
    25261 ggtaagcagc cgtaactctg tgcgtcaggg ctggcgtggc gagcgcgata cccgtggtga
    25321 tacctcctgg gtgccgcctg aaatgattga acgtattgaa gttctgcgtg gtccggcagc
    25381 tgcgcgttat ggcaacggcg cggcgggcgg cgtggttaac atcattacca aaaaaggcag
    25441 tggtgagtgg cacggctcct gggatgctta tttcaatgcg ccagaacata aagaggaagg
    25501 tgccaccaaa cgtaccaact tcagcctgac cggtccgctg ggcgacgaat tcagcttccg
    25561 cttgtatggc aacctcgaca aaacccaggc tgacgcgtgg gatatcaacc agggccatca
    25621 gtccgcgcgt gccggaacgt atgccacgac gttaccagcc gggcgcgaag gggtgatcaa
    25681 caaagatatt aatggcgtgg tgcgctggga cttcgcgcct ctgcagtcac tcgaactgga
    25741 agcgggctac agccgccagg gtaacctgta tgcgggtgat acgcagaaca ccaactctga
    25801 cgcttacact cgatcgaaat atggcgatga aaccaaccgc ctgtatcgcc agaactactc
    25861 gctgacctgg aacggtggct gggataacgg cgtgaccacc agcaactggg tgcagtacga
    25921 acacacccgt aactcgcgta ttccggaagg tctggcgggc ggtaccgaag ggaaatttaa
    25981 cgaaaaagcg gcacaggatt tcgtcgatat cgatcttgat gacgtgatgc tgcacagcga
    26041 agttaacctg ccgattgatt tcctcgtaaa ccaaacgctg acgctgggta cagagtggaa
    26101 tcagcaacgg atgaaggact taagttccaa cacccaggcg ctgaccggaa cgaataccgg
    26161 cggtgctatt gatggtgtga gtgccaccga ccgtagcccg tattcaaaag cagaaatttt
    26221 ctcgctgttt gccgaaaaca atatggagct gactgacagc accatcgtaa cgccggggct
    26281 gcgtttcgat catcacagta ttgtcggcaa taactggagc ccggcgctga acatatcgca
    26341 aggtttaggc gatgacttca cgctgaaaat gggcatcgcc cgcgcctata aagcgccgag
    26401 cctgtaccag actaacccaa actacattct ctacagtaaa ggtcagggct gctatgccag
    26461 cgcgggcggc tgctatctgc aaggtaatga tgacctgaaa gcagaaacca gcatcaacaa
    26521 ggagattggt ctggagttca aacgcgacgg ctggctggcg ggcgtgacct ggttccgtaa
    26581 cgattatcgc aataagattg aagcgggcta tgtggctgta gggcaaaacg cagtcggcac
    26641 cgatctctat cagtgggata acgtgccgaa agcggtggtt gaaggtctgg aaggatcgtt
    26701 aaacgtaccg gttagcgaaa cggtgatgtg gaccaataac atcacttata tgctgaagag
    26761 tgaaaacaaa accacgggcg accgtttgtc gatcatcccg gaatatacgt tgaactcaac
    26821 gctgagctgg caggcacggg aagatttgtc gatgcaaacg accttcacct ggtacggcaa
    26881 acagcagccg aagaagtaca actataaagg tcagccagcg gttggaccgg aaaccaaaga
    26941 aatcagtccg tacagcattg ttggcctgag cgcgacctgg gatgtgacca agaatgtcag
    27001 tctgaccggc ggcgtggaca acctgttcga caaacgtttg tggcgtgcgg gtaatgccca
    27061 gaccacgggc gatctggcag gagccaacta tatcgcaggt gccggggcgt atacctataa
    27121 cgagccggga cgtacgtggt atatgagcat taatactcac ttctgaagct aacgtcagat
    27181 tgttgacaaa gtgcgcgtag ttcatgccgg atgcggcgtg aacgccttat ccggcctaca
    27241 aaaatcttgc caattcaata tattgcagga ccacgtaggc ctgataagcg tagcgcatca
    27301 ggcagttttg cgtttgtcat cattctctaa tatggttgac atgaaaacta cgcatacctc
    27361 cctccccttt gcaggacata cgctgcattt tgttgagttt gatccggcga gttttcgcga
    27421 gcaggattta ctctggctgc cgcactacgc acaacttcaa cacgctggac gtaaacgtaa
    27481 aacagagcat ttagccgggc ggatcgctgc aatttatgcg ctgcgggaat atggctataa
    27541 atgtgtgcct gcaatcggcg agctacgcca acctgtgtgg cctgcggggg tatacggcag
    27601 cattagtcac tgtgggacta cggcattagc cgtggtttcc cgtcaaccaa ttggcattga
    27661 tattgaagag attttctctg cacaaaccgc aagagaattg acagacaaca ttattacacc
    27721 agcggaacac aagcgacttg cagactgcgg tttagccttt cctctggcgc tgacactggc
    27781 attttccgcc aaagagagcg catttaaggc aagtgaaatc caaactgatg cagattttct
    27841 ggactatcag ataattagcc ggaataaaca acaggtcata attcatcgtg agaatgagat
    27901 gtttgctgtg cactggcaga taaaagaaaa aatagtcata acgctgtgcc aacacgatta
    27961 attgacaaca tctggtacga ttcgcccgca gccatcactg ac
//
LOCUS       DAZKHK010000069        27805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKHK010000069 DAZKHK010000000
VERSION     DAZKHK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.069"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            195..1991
                     /gene="narX"
                     /locus_tag="ACHOS5_002870"
     CDS             195..1991
                     /gene="narX"
                     /locus_tag="ACHOS5_002870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP9777661.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANTSGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            1984..2634
                     /gene="narL"
                     /locus_tag="ACHOS5_002871"
     CDS             1984..2634
                     /gene="narL"
                     /locus_tag="ACHOS5_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP9777662.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(2635..4029)
                     /gene="ychO"
                     /locus_tag="ACHOS5_002872"
                     /gene_synonym="iatA"
     CDS             complement(2635..4029)
                     /gene="ychO"
                     /locus_tag="ACHOS5_002872"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP9777663.1"
                     /translation="MSRFIPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVVESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            4214..4567
                     /gene="ychN"
                     /locus_tag="ACHOS5_002873"
     CDS             4214..4567
                     /gene="ychN"
                     /locus_tag="ACHOS5_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP9777664.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(4611..5306)
                     /gene="chaC"
                     /locus_tag="ACHOS5_002874"
     CDS             complement(4611..5306)
                     /gene="chaC"
                     /locus_tag="ACHOS5_002874"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP9777665.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNELLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(5464..5694)
                     /gene="chaB"
                     /locus_tag="ACHOS5_002875"
     CDS             complement(5464..5694)
                     /gene="chaB"
                     /locus_tag="ACHOS5_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP9777666.1"
                     /translation="MPYKTKSDLLESVKHVLPSHVQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            5964..7064
                     /gene="chaA"
                     /locus_tag="ACHOS5_002876"
     CDS             5964..7064
                     /gene="chaA"
                     /locus_tag="ACHOS5_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP9777667.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFV"
     gene            7470..7577
                     /locus_tag="ACHOS5_002877"
     CDS             7470..7577
                     /locus_tag="ACHOS5_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9777668.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(7725..8579)
                     /gene="kdsA"
                     /locus_tag="ACHOS5_002878"
     CDS             complement(7725..8579)
                     /gene="kdsA"
                     /locus_tag="ACHOS5_002878"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP9777669.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(8615..9424)
                     /gene="sirB1"
                     /locus_tag="ACHOS5_002879"
     CDS             complement(8615..9424)
                     /gene="sirB1"
                     /locus_tag="ACHOS5_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP9777670.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(9428..9820)
                     /gene="sirB2"
                     /locus_tag="ACHOS5_002880"
     CDS             complement(9428..9820)
                     /gene="sirB2"
                     /locus_tag="ACHOS5_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP9777671.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(9817..10650)
                     /gene="prmC"
                     /locus_tag="ACHOS5_002881"
     CDS             complement(9817..10650)
                     /gene="prmC"
                     /locus_tag="ACHOS5_002881"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9777672.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIFAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(10650..11732)
                     /gene="prfA"
                     /locus_tag="ACHOS5_002882"
     CDS             complement(10650..11732)
                     /gene="prfA"
                     /locus_tag="ACHOS5_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9777673.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQYVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(11774..13030)
                     /gene="hemA"
                     /locus_tag="ACHOS5_002883"
     CDS             complement(11774..13030)
                     /gene="hemA"
                     /locus_tag="ACHOS5_002883"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP9777674.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQIFGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            13244..13867
                     /gene="lolB"
                     /locus_tag="ACHOS5_002884"
     CDS             13244..13867
                     /gene="lolB"
                     /locus_tag="ACHOS5_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP9777675.1"
                     /translation="MPLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            13867..14718
                     /gene="ispE"
                     /locus_tag="ACHOS5_002885"
     CDS             13867..14718
                     /gene="ispE"
                     /locus_tag="ACHOS5_002885"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9777676.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            14869..15816
                     /gene="prs"
                     /locus_tag="ACHOS5_002886"
     CDS             14869..15816
                     /gene="prs"
                     /locus_tag="ACHOS5_002886"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9777677.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            15941..17620
                     /gene="dauA"
                     /locus_tag="ACHOS5_002887"
     CDS             15941..17620
                     /gene="dauA"
                     /locus_tag="ACHOS5_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP9777678.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(17675..17953)
                     /gene="ychH"
                     /locus_tag="ACHOS5_002888"
     CDS             complement(17675..17953)
                     /gene="ychH"
                     /locus_tag="ACHOS5_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP9777679.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAIFWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            18231..18815
                     /gene="pth"
                     /locus_tag="ACHOS5_002889"
     CDS             18231..18815
                     /gene="pth"
                     /locus_tag="ACHOS5_002889"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9777680.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            18932..20023
                     /gene="ychF"
                     /locus_tag="ACHOS5_002890"
     CDS             18932..20023
                     /gene="ychF"
                     /locus_tag="ACHOS5_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9777681.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFIIYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            20215..20391
                     /locus_tag="ACHOS5_002891"
     CDS             20215..20391
                     /locus_tag="ACHOS5_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777682.1"
                     /translation="MKKTFIFSLLAIVFASLLSACVPHHHHRHNDGPRGPAASGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(20429..22348)
                     /gene="dhaR"
                     /locus_tag="ACHOS5_002892"
     CDS             complement(20429..22348)
                     /gene="dhaR"
                     /locus_tag="ACHOS5_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP9777683.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSTLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDVSQFKRRG"
     gene            22576..23646
                     /gene="dhaK"
                     /locus_tag="ACHOS5_002893"
     CDS             22576..23646
                     /gene="dhaK"
                     /locus_tag="ACHOS5_002893"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9777684.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPLSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            23657..24289
                     /gene="dhaL"
                     /locus_tag="ACHOS5_002894"
     CDS             23657..24289
                     /gene="dhaL"
                     /locus_tag="ACHOS5_002894"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9777685.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            24300..25718
                     /gene="dhaM"
                     /locus_tag="ACHOS5_002895"
     CDS             24300..25718
                     /gene="dhaM"
                     /locus_tag="ACHOS5_002895"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9777686.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFNAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPDSINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETCGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            26037..27734
                     /gene="treA"
                     /locus_tag="ACHOS5_002896"
     CDS             26037..27734
                     /gene="treA"
                     /locus_tag="ACHOS5_002896"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9777687.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSATGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQKEAEPTP"
BASE COUNT         6834 a         7193 c         6916 g         6862 t
ORIGIN      
        1 tatgagattt ttcacggtgc tgtaaaatcc ctacccttac cgatgtaaag cgactaacca
       61 cacggcaaat aaggagtaac tctttccggg tatgggtata cttcagccaa tagccgagaa
      121 tactgccatt ccagaatgta tcgtcacatt cattaaggtt attgctcatt taaagcctga
      181 aggaagaggt ttacatgctt aaacgttgtc tctctccgct caccctggtt aatcaggttg
      241 cgcttattgt gttgctttct actgctattg gactggcagg gatggcggtt tctggctggc
      301 tggtgcaagg cgttcagggc agcgcccatg cgatcaacaa agcgggatcg ctgcgcatgc
      361 aaagttaccg tctgttggca gcagtgccat taagcgagaa agacaagccc ttaattaaag
      421 agatggaaca aacagcattt agcgccgagt tgactcgagc agcagaacga gacgggcaac
      481 tggcgcaatt acagggttta caagattact ggcgtaatga actgatccct gcactgatac
      541 gtgcacaaaa ccgcgaaacg gtgtcagcgg atgtcagcca gtttgttgcc gggcttgatc
      601 agctggtatc tggttttgat cgcaccacgg aaatgcgcat tgagacagtg gtgctggtcc
      661 atcgggtaat ggcggtattt atggcacttt tactggtatt cactattatc tggttgcggg
      721 cgagattgct acaaccgtgg cggcaactgc tggcaatggc gagtgccgtc agccatcgcg
      781 attttaccca acgcgcaaac accagcgggc gcaacgaaat ggcgatgctt ggaactgcgt
      841 tgaacaatat gtctgcagaa ctggccgaaa gttatgccgt acttgagcag cgggttcagg
      901 agaaaaccgc cgggctggag cataaaaatc agatcctctc ttttttatgg caggctaacc
      961 gacgtttgca ttcccgcgcc ccgctgtgtg aacgcctgtc acctgtactc aacggattac
     1021 agaatttaac cctgctacgt gatatcgaac tgcgggtgta tgacactgat gatgaagaga
     1081 atcatcagga gtttacctgc cagccagata tgacttgtga tgataaaggc tgccagctct
     1141 gcccgcgcgg cgtattaccc gttggcgatc gcggcacaac cctgaagtgg cggctggctg
     1201 acactcatac gcagtacggt attttgctgg cgaccctgcc gcaggggcgt catcttagcc
     1261 atgatcaaca acaactggtg gataccctgg ttgaacaact caccgccacg ctggcgctgg
     1321 atcgccatca ggaacgtcag caacagttga tcgtgatgga agagcgtgcc accattgcgc
     1381 gcgaactgca tgattctatt gcccaatctc tctcttgcat gaagatgcag gtgagttgct
     1441 tacagatgca gggcgatgcg ctgccagaaa gcagccgcga actgttaagt cagatccgta
     1501 acgaactgaa tgcatcctgg gtgcagttgc gtgaattgct caccacattc cgtttgcagc
     1561 tcaccgagcc tggattacgt ccggcgctgg aggcgagttg cgaagagtac agcgccaaat
     1621 ttggcttccc ggtgaagctg gattatcaat tgccgcctcg tctggtgcct tcgcatcagg
     1681 caatccactt gttgcaaatt gcccgtgagg cattaagtaa cgccctcaaa cattcgcaag
     1741 cgagtgaggt cgtggtgacg gtggcgcaaa acgataatca ggtcaaactg accgtccagg
     1801 ataacggctg cggcgtgcct gaaaatgcca tccgcagcaa tcactacggc atgataataa
     1861 tgcgcgaccg tgcgcaaagt ttacgaggcg attgccgcgt ccgccgtcgt gaatcaggtg
     1921 gcaccgaagt ggtggtcacc tttattcccg aaaaaacttt cacagacgtc caaggagata
     1981 cccatgagta atcaggaacc ggctactatc ctgctgatcg acgatcaccc gatgttgcga
     2041 actggcgtaa aacagcttat cagtatggca ccagatatca ccgtggttgg cgaagcgagt
     2101 aatggcgaac agggtattga actggcggag tctcttgatc ccgatctgat cctgttagat
     2161 ctcaatatgc ccggcatgaa cggtctggaa acgctggata aactgcgcga aaagtccctc
     2221 tccgggcgca ttgtggtatt cagcgtctct aaccatgaag aagatgtggt caccgcactg
     2281 aaacgcggcg cggatggcta tctgttaaaa gatatggaac cggaagatct gctgaaagcg
     2341 ttacatcagg cagctgctgg cgaaatggta ttaagcgaag cattaacgcc tgttctggcc
     2401 gccagcttgc gcgctaaccg tgccactact gagcgcgatg ttaaccagtt aaccccacgc
     2461 gagcgcgata ttctcaagct aattgcccag ggtttgccga acaagatgat tgcccgccgc
     2521 ctggacatca ccgaaagcac agtaaaagtt cacgtcaagc acatgctgaa gaaaatgaag
     2581 ctaaagtctc gcgtggaagc tgcggtatgg gtgcatcagg agcgcatttt ctgattacgg
     2641 ttcccagcgc agttcgtcgt ttgacaatgc gtcgaacggt tcgacaagcg ttagcgtgat
     2701 ctcattggag gagacacgct gcccctggtt atcttccacc accactgaca atcgccagtg
     2761 attgcttgcg ccttccccgt tttgccagtc aggcatgatc agcgtccagc cctcctcact
     2821 gttggcttgt gcgcccggcg tcaaacttaa tatctgcgta tcgccctgcc aaatcagttg
     2881 ccgaataccg taacgactgc ggatttgtaa tttcagcggc actgtttcgc caggttttag
     2941 atcccacggc ggtgtcgcca gaaacaccgt taacgttttt cgctgtcggt actcaagagt
     3001 cggcagatta tttcgctgcg gattgtcata gcgactacca cgtaacgact gactttcgac
     3061 aacctcgccc gcagaaagtt gttttttgag cggtacacca aagcggtagt taagattcag
     3121 cccgaggtta ttttgattct cgccactttc accctgttta tgctgggccg tcacagtgac
     3181 taagggcaca ggggtgtaat ttaatcccag actcaacgcg acgggattgt gataacccgt
     3241 accagagtta aacaaatcga cacgatcacc aaaatactgt tctacgctga cactggtatt
     3301 gaggtgttga tagaacggca tgcgcatccg ggctgtcagg tcgtacccgc gcgccatccg
     3361 ttgttcctgc gtggctgtct gttcatgcca tgcagcaaac ggctgataaa agtttgccga
     3421 tagtcgcaaa tattcgcccc acgcttcagc gccaaagccc gctcgctgaa gattttcgtc
     3481 cagcaagttg tcataaaaag tgttataacc caccagccag ctgccgcgcg cccagcgttg
     3541 cccaacgccc acattgctca ccagcccatc atcctgctga gtaagaccaa gctggctcca
     3601 ggtgagataa cgatcattat cttgtaacgg cacaaaccag cttccacgac tgccggtgaa
     3661 atgtccttcg ttatccactt tgacgtcaac actggcattt ccccacggtg atagccagga
     3721 ctctacgtgc tgattaacct gttggctaag cgcgtcgcgg acctttccca atgcgaaagc
     3781 ttttgcctgc tcgccagtat caagcccgtt atcattcata ctggtttcgc cgaaatcttt
     3841 cacaatttca gcaaagtgtt tttccccatc atgattttca ggtgccatgc ctaaatccgg
     3901 cagaccatca ttgttattat caaagggatt tgccgctttt tgctcgaagg tagattgtgc
     3961 gttagctgta ccgcctgccg ccagcaagag taaataaaac ggaataatgc gaggaatgaa
     4021 acggctcaaa acgtcggtaa cttccggtaa aaatgaaaaa ccatagcctt aaagaataac
     4081 gattactggc accttttgtc gtattttctc gcgctttcag gaaaatcctt aaaaatgtga
     4141 ccgattggtc agacgctgca gaagcagtaa gataggagat gagtgtctaa ttccctgaat
     4201 acaggagtaa ataatgcaaa aaatcgtgat cgttgccaat ggcgcacctt acgggagcga
     4261 atccttgttt aacagcttgc ggctggccat tgcgttacga gagcaggaga acaatctgga
     4321 tctgcgtctg ttcctgatgt ctgatgcggt aacagccggg ttgcgcgggc aaaaaccagg
     4381 ggaaggctac aacattcagc aaatgctgga gatccttacc gctcagaatg taccggtgaa
     4441 attgtgcaaa acctgtaccg acgggcgcgg gattagtaca cttcctctga ttgatggggt
     4501 ggaaatcggt actcttgttg aactggcgca atggacgctg tcagccgata aagtgctcac
     4561 attttaatgt ttgagggggc gtcggacgcc ccactatgag gaaagtttac tcaggctaat
     4621 cccggccgta acacaccatc cggataattc tccgccagca gtttttttac cgataccagc
     4681 aattcattca gcccatcgtc ctgcattccc agtttgataa gctcctgttc cagtgaaaac
     4741 aggtattgtg cattggttcc cagcggaccg ctcgccgccg caatcaacgg cgctatgacc
     4801 tgagcgcgag tgtcagattc atattctgga tgtcgcgggt ccataataaa cacaatggcg
     4861 tttactgtgc gtccatcatc aagatcaagc tgacaccagg ttggcagata acagccggta
     4921 atcatctcac gcttccacag cagggttagt tcctgctcca gcgtctcttc tggcagtcga
     4981 taggcgacgc ctgtggtgcg tccgccctct ttcagtgcaa gcattcgtcc cggctggtgc
     5041 gcagtcccgc gcccggcggt cagacgcagg caaaacgcgc gatgccatcc aaccagtgta
     5101 ccggtgcacg actcggtaaa ctccagtgcc ggattccaca tcaacgatcc ataaccgaag
     5161 atccacaccg gtccctcatc aggtcgacaa gccagcgtcg ccgccagcga agccgcccgt
     5221 tgttctgctg accataagag tgattcttca atggcaccaa atgccgtttt acaatcggca
     5281 ttcatcaaga aatcacgcgt tatcacctta cacctccgcc actgcatact tccctgcgac
     5341 aactttttgg tgtcttccgg acatttttac tgctcatttt gaaaccacta acgggacaat
     5401 atgcaatcca cggaccactt gcaaggcaac accgcacgaa tagctttaac taagctaccg
     5461 gttttacgat tttttatgcc atttatcatc atcccctttg gcatattcat gcttcacagc
     5521 agcccaggcc actttatgcg ctgtttcttc gcgactggcg tcatcacgcc gatcttcttt
     5581 atctttatat tgatcccatg cgctgttgaa cgcttcttta tagatatcct ggacatgaga
     5641 cggaagcacg tgctttacgc tttccagcag atcgcttttc gttttatacg gcatcgttga
     5701 ctccttttac aaagttgaac attaagtgtg gtcaacaata tctactcttg ccaaaacata
     5761 agaattttca tgtcagactc ttcttaagaa gtgctaaaac aaacattgtc taaacgtatt
     5821 acttattttt atgcccaaat gagtaaaaaa tcgacagtgg ggtaaaaatt cgtaaaatgt
     5881 taccgatatc cagacacttt ctgttatttt tcctacctat tttttacatt ttcgataaat
     5941 tcatctgcaa agggataacc ataatgtcaa atgctcaaga ggcggtaaaa acccgccaca
     6001 aggagacttc gcttattttc ccggttctgg cgctggtagt gctgttcctg tggggaagca
     6061 gccagacact accagtggtc attgccatca atcttcttgc gcttattggt attttaagta
     6121 gcgcctttag tgttgtccgt catgcggacg tattagccca tcgcctggga gaaccttacg
     6181 gttcgcttat tcttagcctt tcagtggtta ttcttgaagt cagtttgatt tcagctttaa
     6241 tggcaaccgg cgacgccgcg ccaacgctaa tgcgtgatac gctctattca atcattatga
     6301 ttgttacagg tgggctggtt ggcttttcat tattgttggg cggacgtaag tttgccaccc
     6361 aatatatgaa tctgtttggt atcaagcagt atttaattgc gctgttcccc ctggcgataa
     6421 tcgtactggt atttccaatg gctctgcctg cggcgaattt ttcaacaggt caggcgttac
     6481 tggtagcatt aatttctgcg gcaatgtatg gcgtattttt gctgatccag accaaaacac
     6541 atcaaagttt gtttgtctac gagcacgaag atgacagtga tgatgacgac ccgcaccacg
     6601 gtaaaccgtc tgcccatagc agcctgtggc atgccatctg gttgattgtc catctgattg
     6661 ccgttattgc ggtgaccaaa atgaacgcca gcccgctgga gacattgctc gacagcatga
     6721 atgcccctgt cgcctttact ggcttcctgg tggctctgtt gattctgtcg ccggaaggtt
     6781 taggtgcgtt aaaagcagtg ttgaacaacc aggtccagcg cgcgatgaat ctgttctttg
     6841 gttcagtgtt agcaaccatt tcgcttaccg tacctgtcgt cacgctaatt gcctttatga
     6901 cgggtaacga attgcagttt gcacttggtg cgccagaaat ggtggtgatg gtggcctctt
     6961 tagtgctgtg ccatatctcc ttctccaccg gacgaactaa cgtgctcaat ggcgcagcgc
     7021 atctggcact gtttgccgcc tatttgatga cgatatttgt ctgacattta tatctcctcc
     7081 gatgacggaa atcgggggag ataatataaa ttcctgctta ttgtatttct tcatactacc
     7141 tggacaggtc gttaatcaga attgcgagcc tgtaccacaa cctgcacgaa accactgcaa
     7201 cagctgttga aaagccggaa tatactcttc aatgcaaaca tttcagggat gaagtgaaga
     7261 tgaattgcga cgtagtatcc acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg
     7321 gggctttatc cctggtggca ttggttgctg gaaagagaaa acccccgcac gttgagaggt
     7381 aaaacctgac aacagaacgg gggctaatct tgaaccatca acaattcaag aatagccgca
     7441 aaatgttgtc attacaacaa ggcggctata tgacgctcgc gcagtttgcc atgattttct
     7501 ggcacgacct ggcagcaccg atcctggcgg gaattattac cgcagcgatt gtcggctggt
     7561 ggcgtaaccg gaagtaaggt gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc
     7621 gaaaccggcc gcaggggtaa cagcctgcgg ccttttttgt atccggcacc cataaaaaaa
     7681 gtcttaacgc agaacgctaa gactttattt ttcaaacaaa aagattactt gctggtatcc
     7741 agttcttcga aacctttcac cagatcatca atcgctttca tttgcttgag gaacggttcc
     7801 agtttagcca gcggcagcgc ggatggacca tcacatttcg catgttccgg atccggatgc
     7861 gcttcaataa acagccccgc cagacctacc gccataccgg ctcttgccag ctcagccacc
     7921 tgagcacggc gaccgccgga agctgcgcca aatggatcgc gacattgcag tgcgtgggta
     7981 acgtcgaaaa tcaccggcga attaccagac actttcttca tgatgctgaa gcccagcata
     8041 tcaacaacca ggttgtcata gccgaagtta gcaccgcgat cgcaaagaat cactttttcg
     8101 ttgccgcctt ctttgaattt atcaacgata ttacccatct gtcccgggct gacaaactgc
     8161 ggtttcttga cgttaattac cgcaccggtt ttcgccatgg cttcaaccag gtcagtctgg
     8221 cgagcaagaa acgccggcaa ctgaatcaca tctacaacat cagcaacggg ctgtgcctgg
     8281 cttggttcgt gaacgtcggt gataattttc acgccaaaag tctgcttcaa ctcctggaag
     8341 attttcatcc cttcttccag gcccggtcca cgataagagt ggatggagga gcggttggct
     8401 ttgtcaaaag aggctttgaa cacgtaaggg atacccagtt tctgggtcac ggttacgtag
     8461 tgctcgcaaa tgcgcatcgc cagatcgcga gattccagca cgttcatacc gccaaacagt
     8521 acgaacggca ggtcatttgc tacgttgatg tcgccaatgc taaccacttt ttgtttcata
     8581 ggatcgcctt aatcttgagt aaaatgtcga ttaattaatg cagcacaata tgtttatgcg
     8641 cgatgttatt tatttgcgca cggatcattt cgctgatcgg gtcttccgga cactgttcaa
     8701 cgaaataact taaatcgttc aacgcaacgt gttcgcaatc cagttgcgca taaatcaacc
     8761 cgcgatcgcg aatttcatag ggatcttccg ggttgaattg taataaagct tcgctggtgc
     8821 gtaacgccag ctccatctga ttttcttcca tcaacgaagc tttgagtgta tccagcaatt
     8881 tgcggattac ctcaatgtta tcagcttcat caaggtcttc ataaaacagt tccgccgacg
     8941 ggctgatatt tccctttaac cacacatcca gcatatgttc gcttaacgat tcaccgttaa
     9001 aaggattaat cagccaaatt tcgccatccg gacattcaat gcgcaatatc agctgcgtag
     9061 ggaaaatcac cggcagcagc ggcaaatcga gacgattcgc gacccataat aaaaccgcac
     9121 ctaatgatac cgcactgccc tgtcgattct ttaacacctg gtccagccat aatgcatcgg
     9181 aaagacgata aacgccgcgt gaggctttaa acccccattc gccgtaaaac agcgcgatta
     9241 atttttctag ctgctcttct aatggcagaa gctggctaat ttcttccttc gccagactaa
     9301 cgagacgctc cagttcgtcg taaacatctt gcgagggaaa atcgcggcgg attgcttcgc
     9361 aagccaggat catgccttcg cacaatggcg ctttattaaa ttcgaaatca gctaacgatc
     9421 tcatgactta ccccagtaac ggtacttttg tggtggcgag tttaatgatg atgtacagca
     9481 ccaccaacgc cagcgggaag gcaataatgc gcgcctgctg actgtgcata cgacgataat
     9541 cgagtgcaat aaaacccaaa acaatataaa tgataactcc aaacagcttt tcagtcagcc
     9601 actgtgcctg cccggaaaat ggctggatgt gcgctttagc catcaacgca atgccgctta
     9661 acaataacag cgtatcgaca accggcggca caatgcgcgt ccagcgagca aatgcctgtg
     9721 gatgcttctg ataacgtagc cagaaacgta aggtcaatag cccaacagaa agcgcgatac
     9781 taatgagatg aacgctaagc aacgtagaaa aacttgtcat tgataatagc ggccgagcgt
     9841 tacgcgctcg ttatccccat agtcacggca ggtttcgacg tcatgatacc ccgcgaagat
     9901 aaatgcctgt cgcaccgctt caccctgctg ccagccatgc tccagaagca gaaagccgcc
     9961 ggataccagc gcgttacgcg actgttcgat gatatgcacg atgtctgcca ttccactgtc
    10021 tgccgcaacc agcgcagtga gcggctcaaa gcggacatcg ccttgttgaa gatgtgggtc
    10081 ctgctcgtca atatacggcg gattgctgac aatcatcgca aactgctgcc cggctagcgc
    10141 gctaaaccag tcgctttgca gaatgtggat atttttgata gccagatgct gggcattacg
    10201 ttgcgccaga gagacagcat ctggcatacg atctacagcg gtaatttcgc agtccggacg
    10261 ctcgctggcc agcgccagcg caatcgcccc ggtacccgtc ccgagatcga gaatacggca
    10321 aggttgttca ggcaaccgcg ccagtgcctg ctccaccaga cactccgtat ccgggcgcgg
    10381 aattaaggtc gctggcgaaa caaataacgg caacgaccag aattctcgca ccccggttaa
    10441 atgagcaatg ggttcaccat cgcgacgacg cgtcagtagc gcatcaagtt gctgacattg
    10501 ttcgtcagtc agttgcgttt caccaaaggc gagaataaaa gtacgccctc tgccggtaac
    10561 atgctccagc aggatttcag catcacgccg cgggctttcg ctcgcctgaa gttggcttat
    10621 tgcttcacgt aaccagtgtt gatattccat tattcctgct cggacaacgc cgccagttgg
    10681 tcggcctgat gttcctggat aatcggttca atcagcatat ccagcttacc ttccatcact
    10741 tcatccaggc ggtagagcgt caggttgatg cggtgatcgg taacgcgccc ctgcgggaag
    10801 ttgtaagtac ggttacggtc gctgcgatcg ccactcccca gcaggttacg acgggtagac
    10861 gcttcggcct gttgccgttt tgccatttca gcagcgtgga tgcgagcacc gagtacagaa
    10921 agtgctttag ctttgttttt atgttgtgaa cgttcgtcct gacattcaac aacaatcccg
    10981 gtcggcaagt gagtaatacg aattgccgaa tcggtggtgt taacgtactg accacccgcc
    11041 cctgacgagc ggaaagtatc aatgcgcaaa tctgctgggt tgatgtccgg cagttctgca
    11101 tcaggcagtt ctggcattac cgcaacggta caagcagaag tatgaatacg accctgcgat
    11161 tccgtagcag gaacacgctg cacgcgatga ccgccagatt cgaatttcaa acgaccatat
    11221 acaccatcac cgctaatttt ggcgatgatc tctttataac caccatgttc accctcgctg
    11281 gcgctcatga tttctacacg ccagcggcgg gcttcggcgt aacggctgta catacggaac
    11341 agatcgccag cgaacagcgc cgcttcgtcg ccgccggttc cggcacggac ttcgaggaag
    11401 gcgttacgtt cgtcatcagg atcttttggt agtaacagaa cctgtaattg ctgttccagt
    11461 tgctcgcttt tttctttagc ttcgcgcagt tcatcctgcg ccatctcacg catttcagga
    11521 tcgtcgagca tcatctgtgc ggtttcgata tcttcctgaa cctgttgcca gtcggtaaaa
    11581 cagcgcgaaa catcacttaa ctgcgcatat tcacgagata atgcgcgaaa acgttcctgg
    11641 tcagcgatgg tttgcgcatc acccagcagc gcctgaactt cttcatggcg ttcatgcagg
    11701 gcttccagtt tggcaacgat agaaggcttc ataggcgtaa atgcaccctg taaaaaaaga
    11761 aaatgatgta ctgctactcc agcccgaggc tgtcgcgcag aatattcagg cgttcgttat
    11821 ccccgtcacg ggcagcctgt tgaagtgatt ttgttggtgc atggatcagg cggttagtca
    11881 gtttccatgc cagatcctgc ataatggttt gcgcatcacc gccctgttca agggccgcta
    11941 acgctttagc agttaactca tcacgaacct gctctgcctg gctacgataa tcgcgaatag
    12001 tttcgctggc gctttgtgct cgaagccatg ccataaattc actggcctcc tgggcgacga
    12061 tagtttccgc ctccacagca gcagctttcc gttgcgccag gttgtgcgaa ataatgcttt
    12121 gcagatcgtc aacgctataa agataagcat tcgccagttt accaacttcc ggctcaacat
    12181 cgcgcggaac ggcaatatcc accagcagca ttggttgatt gcgacggctt tttaatgcgc
    12241 gctccaccat gcctttaccg ataatcggca acgggctggc ggtggaactg atgatgatat
    12301 cggcttcgcg cagacgttcg tcgatatcgc tcagggcaat cacttccgcg ccaacctcat
    12361 ctgccagaat ttgggcacgt tcgcgagtgc gattggcgat aatcatcttc tgtactttgt
    12421 gttcgcgcag atgacgcgcc accagctcga tggtttcgcc cgcgccaacc agcagtaccg
    12481 tcaccgtaga gagcgattca aagatctgcc gcgccagcgt acaagccgca aaagcgacag
    12541 acacagcact ggcaccgata tctgtttcag tgcgaacgcg tttcgccaca gagaaagatt
    12601 tctgaaacat gcgttccagt tcgctggcct tcatatgacc tttttgcgaa tcggcaaacg
    12661 cttttttcac ctgaccgaag atctgcggct cgccaaggac gagagaatca agcccactgg
    12721 caacgcgcat taaatgactg accgcatcgt tatcctgatg ccagtagagg ctattacgca
    12781 gatcgtcttc gttgagatta tggtaatcac acaaccagcg aatgagcgcc tcttgcaggt
    12841 catcccgttc ttcaacgctg agataaagtt ctgtgcggtt acacgtcgac agcaccacac
    12901 cgccctgcac catcggctgc gcgagcagac tgtcaagcgc ctgatcgagc ttatccggcg
    12961 aaaacgatac acgttctcgc agcgatacag gtgccgtttt atggttgata ccgagtgcta
    13021 aaagggtcat gtctgcggga aataatacca acgttgatag ggttagtctg cttgcatcat
    13081 acaggatgcg taagatcaat aaaagagagc gccccctttt ggagtaattg ccgaaagccg
    13141 ttagattctg gcaattaaga caacgtgaac atagacgatg gctaacggta acgctagcat
    13201 taagggttat aactgcaacg tatctcaagg acttgtcatt attatgcccc tgcccgattt
    13261 tcgctttatc cgcctgctac cgctagcagc cctcgtgctc actgcctgtt ctgttaccac
    13321 acccaaaggc ccaggcaaaa gcccggattc gccacaatgg cgtcagcacc agcaagacgt
    13381 gcgcaatctt aatcagtatc aaactcgcgg cgcgttcgct tatatttctg accaacaaaa
    13441 agtgtacgcc cgtttcttct ggcagcaaac tggccaggat cgctaccgtc tgctgctgac
    13501 taacccattg ggcagcacgg aactggagct gaatgctcaa ccgggtaacg tgcagttagt
    13561 cgacaataaa ggtcagcgtt atacctccga tgacgccgaa gagatgattg gcaaattgac
    13621 cggaatgcca attccgctca acagcttgcg ccagtggatt ttaggtttac cgggtgatgc
    13681 gactgactac aaacttgacg accagtaccg cctgagtgaa atcacctaca gccagaatgg
    13741 caaaaactgg aaagttgttt acggtggtta tgacacaaaa actcaacctg cgatgccagc
    13801 caatatggaa ctcaccgacg gtggtcaacg catcaagtta aaaatggata actggatagt
    13861 gaaataatgc ggacacagtg gccctctccg gcaaaactta atctgttttt atacattacc
    13921 ggtcagcgtg cggatggtta ccacacgctg caaacgctgt ttcagtttct tgattacggc
    13981 gacaccatca gcattgagct tcgtgacgat ggggatattc gtctgttaac gcccgttgaa
    14041 ggcgtggaac atgaagataa cctgatcgtt cgcgcagcga ggttgttgat gaaaactgcg
    14101 gcagacagcg ggcgtctttc gacgggaagc ggtgcgaata tcagcattga caagcgtttg
    14161 ccgatgggcg gcggtctcgg cggtggttca tccaatgccg cgacggttct ggtggcgtta
    14221 aatcatctct ggcaatgcgg actaagcatg gatgagctgg cggaaatggg gctgacgctg
    14281 ggcgcagatg ttcctgtctt tgtccgtggt catgccgcct ttgccgaagg tgttggcgaa
    14341 atattaacgc cggtagaccc gccagagaaa tggtatctgg tcgcacaccc tggcgtgagt
    14401 attccgacgc cggtgatttt taaagatcct gaactcccgc gcaatacgcc aaaaaggtca
    14461 atagaaacgt tgctaaaatg tgaattcagc aatgattgcg aggttatcgc aagaaaacgt
    14521 tttcgcgagg ttgatgcggt gctttcctgg ctgttagaat acgccccgtc gcgcctgact
    14581 gggacagggg cctgtgtctt tgctgaattt gatacagagt ctgaagcccg ccaggtgcta
    14641 gagcaagccc cggaatggct caatggcttt gtggcgaaag gcgttaatct ttccccattg
    14701 cacagagcca tgctttaagc cgggcaagct gagtttcggt gacaacgtca ccttgttcca
    14761 gacgttgcat cgcgctcttt aatacaccgc ctggaaagaa tcatgcctgg cccgcacagt
    14821 tttcggcaga ttctttccac caatggacgc atgcctgagg ttcttctcgt gcctgatatg
    14881 aagctttttg ctggtaacgc caccccggaa ctagcacaac gtattgccaa ccgcctgtac
    14941 acttcactcg gcgacgccgc tgtaggtcgc tttagcgacg gcgaagtcag tgtacaaatt
    15001 aatgaaaatg tacgcggtgg tgatattttc atcatccagt ccacttgtgc ccctactaac
    15061 gacaacctga tggaattagt cgttatggtt gatgccctgc gtcgtgcttc cgcaggtcgt
    15121 atcaccgctg ttatccccta ctttggctat gcgcgccagg accgtcgcgt ccgttccgct
    15181 cgtgtaccaa tcactgcgaa agtggttgca gacttcctct ccagcgtcgg tgttgaccgt
    15241 gtgctgacag tggatctgca cgctgaacag attcagggtt tcttcgacgt tccggttgat
    15301 aacgtatttg gtagcccgat cctgctggaa gacatgctgc agctgaatct ggataaccca
    15361 attgtggttt ctccggacat cggcggcgtt gtgcgtgccc gcgctatcgc taagctgctg
    15421 aacgataccg atatggcaat catcgacaaa cgtcgtccgc gtgcgaacgt ttcccaggtg
    15481 atgcatatca tcggtgacgt tgcaggtcgt gactgcgtac tggtcgatga tatgatcgac
    15541 actggcggta cgctgtgtaa agctgctgaa gctctgaaag aacgtggtgc taaacgtgta
    15601 tttgcgtacg cgactcaccc gatcttctct ggcaacgcgg cgaacaacct gcgtaactct
    15661 gtaattgatg aagtcgttgt ctgcgatacc attccgctga gcgatgaaat caaatcactg
    15721 ccgaacgtgc gtactctgac cctgtcaggt atgctggccg aagcgattcg tcgtatcagc
    15781 aacgaagaat cgatctctgc catgttcgaa cactaatcga acccggctca aagacccgct
    15841 gcggcgggtt tttttgtctg taatatccat ttgtatgacc tatgcctcct tcacctgcca
    15901 tttagttgac agatgatgcg ctcacggatg aaacattatt gtgaacaaaa tattttcctc
    15961 acatgtgatg cctttccgcg ctctgatcga cgcttgctgg aaagaaaaat atactgccgc
    16021 acggtttacc cgtgacctga ttgccgggat aaccgtcggg attattgcta tcccgctggc
    16081 gatggcgttg gctattggta gtggtgtggc accccagtac ggtttatata ccgcagctgt
    16141 tgcggggatt gtcattgctc tgacgggagg gtcacgcttt agcgtttccg gtccgactgc
    16201 ggcatttgtg gtaattctct atcccgtttc gcaacagttt ggactggcag gactgctggt
    16261 tgcgaccttg ctgtcgggga tctttttgat tctgatgggg ctggcacgct ttggtcgcct
    16321 gattgagtat attccggttt ccgtcacctt aggtttcacc tcgggtatcg ggatcaccat
    16381 cggtaccatg cagattaaag attttctcgg tctgcaaatg gcccatgtcc cggaacatta
    16441 tctacaaaaa gtcggcgcat tatttatggc gctgccgacc attaatgtgg gtgatgcagc
    16501 cattggcatt gtgacgcttg gcattctggt tttctggccg cgtctgggca tacgtttacc
    16561 cggccacctt ccggccttgc tggctggttg cgcggtgatg gggattgtta acctgctcgg
    16621 cggacatgtt gctaccatcg gttcgcaatt ccactacgtc ctggccgatg gttctcaggg
    16681 taacggtatt ccgcaactgc tgccgcaact ggtgctgccg tgggatctgc ctaattcaga
    16741 attcacgcta acctgggatt ctattcgcac cctgctgcct gcggcattct caatggcaat
    16801 gctcggcgca atcgaatctc tgctctgcgc cgtggtgctg gatggtatga ccgggacgaa
    16861 acacaaggcg aacagcgaac tggttggaca gggactgggg aatattatcg ctccgttctt
    16921 tggtggtatt accgctacag ctgccatcgc gcgttctgcc gctaacgtcc gtgccggggc
    16981 aacttcccct atctcggcgg tgatccactc tattctggtt attcttgccc tgctggtact
    17041 ggcaccgctg ctctcctggc tgccgctttc cgccatggca gccctgctgt tgatggtggc
    17101 gtggaacatg agtgaagcgc acaaagtggt cgacttgctg cgtcatgcac cgaaagatga
    17161 catcatcgtc atgctgctgt gcatgtcgct gaccgtgctg ttcgatatgg ttattgccat
    17221 cagcgtgggg atcgtgctgg catcgctgct gtttatgcgt cgtatcgcac gtatgactcg
    17281 cctggcaccg gtagtcgtag acgttccaga cgatgtcctg gttctgcgcg ttattggccc
    17341 gctgtttttt gctgccgcag aaggcttatt cacggacctg gagtcacgtc ttgaaggcaa
    17401 acggattgtg attctgaagt gggatgccgt tccggtactt gatgctggtg gtcttgatgc
    17461 gttccagcgt tttgtgaagc gtctgcccga aggatgtgaa ctgcgcgtgt gcaacctgga
    17521 attccagcca ctgcgcacta tggctcgcgc aggcattcaa ccgatcccgg gacgcctcgc
    17581 attcttcccg aatcgtcgcg cggcgatggc ggatttataa gtacgagatt gaccagtcag
    17641 cgcaaactga ctggtcagca aactgcatta tttgttagct atgacggcga ttgtcgctac
    17701 ggcggcaacg tttgtcatag tggcgaaccc accagtaacg gtcgcacacc tgttcatgcc
    17761 cgccaacacg ggcacccgcc agccagaaaa tcgcaccgac gaaaatactt agcacagcac
    17821 catgtgcgaa atactggggc atattaaact gtggtaactg gttgaggatt gaatacccca
    17881 cgccgaccac cattaccacc agacccaacc ccatgagcac gttaccgagt aacgaagcgt
    17941 ttttgcgttt catatgtcac ctccggaact ttctgggttg taacagggaa tacccctctt
    18001 ccttatgtgt aaagtataga caacaccctg taggttatgt gcgggtgtga tcacaattac
    18061 aacccttatt tcaacaaaac tttacaaata aacgcctgaa caccttgact ttctaatagt
    18121 ccactcaggc aataattgca ttatctgcat ttgatacgca gttttttgtc aagcggggcc
    18181 gcaaccagta aactacgcgc cagttatgta cacactcagg acaaaaaaac gtgacgatta
    18241 aattgattgt cggcctggcg aaccccggtg ctgaatacgc cgcaacgcga cataatgctg
    18301 gtgcctggtt cgttgactta ctggcagagc gattgcgcgc tccgctgcgc gaagaggcta
    18361 aattctttgg ttatacttcg cgagtcaccc ttggaggcga agatgtccgc ctgttagtcc
    18421 cgactacatt tatgaatctc agcggcaaag ccgttgcggc gatggccagt tttttccgca
    18481 ttaatccgga cgaaattctg gtggcccacg acgaactgga tctgcctcct ggcgtcgcca
    18541 aatttaagtt aggtggcggt catggtggtc acaatggact gaaagacatc atcagtaaat
    18601 tgggtaataa ccctaacttt caccgtttac gcatcggaat cggtcatccg ggcgataaaa
    18661 ataaagttgt cggttttgtg ttaggcaaac cgcctgttag tgaacagaag ttaattgatg
    18721 aagccatcga cgaagcggcg cgttgtactg aaatgtggtt tacagatggc ttgaccaaag
    18781 caacgaaccg attgcacgcc tttaaagcgc aataagtcgt tgtctgcggc atttttgccg
    18841 agtgccgtgt ataataggca aagttatttc catttctgca atctgttagc aataacaggt
    18901 tgattattaa gatattaagg tgatttaaat catgggattc aaatgcggta tcgtcggttt
    18961 gcccaacgtc gggaaatcta ccctgttcaa cgcgctgacc aaagccggta ttgaagcggc
    19021 caactttcca ttctgcacca tcgagccgaa cactggcgtc gtaccaatgc ctgaccctcg
    19081 cctggatcaa ctggctgaaa tcgtaaaacc gcagcgtaca cttcccacga ccatggaatt
    19141 tgtcgatatc gccggtctgg taaaaggcgc atcgaaaggc gaaggtctgg gtaaccagtt
    19201 cctgaccaac atccgtgaaa ccgaagcgat cggtcacgtt gttcgctgct ttgaaaatga
    19261 caacatcatt cacgtttccg gcaaagttaa cccggcggat gatattgaag ttatcaacac
    19321 cgaactggcg ctggcggatc tcgacacctg tgaacgtgcg attcatcgcg tacagaagaa
    19381 agccaaaggt ggcgataaag acgcgaaagc cgagctggcg gtactggaaa aatgcctgcc
    19441 ccagttggaa aacgcaggta tgctgcgtgc gctggattta agcgctgaag agaaagcggc
    19501 tgttcgttac ctgagcttcc tgacgctaaa accaacaatg tacatcgcca acgtcaacga
    19561 agacggtttt gaaaacaacc cgtatcttga ccaggtgcgt gaaatcgcag cgaaagaagg
    19621 ttctgttgta gttccggttt gtgctgctgt tgaagcagac attgccgaac tggacgacga
    19681 agagcgtgac gagtttatgc aggagcttgg gctggaagag ccgggcctga accgtgtgat
    19741 ccgtgccggt tataagctgc tgaacctgca aacttacttc accgctgggg tgaaagaagt
    19801 gcgtgcatgg accattccgg ttggtgcaac cgcgccgcag gcagcgggca aaatccatac
    19861 tgattttgaa aaaggcttta tccgtgcaca aaccatctcg tttgaagatt tcatcattta
    19921 caaaggtgaa caaggcgcga aggaagcagg caaaatgcgt gcagaaggta aagactacat
    19981 cgttaaagat ggcgatgtga tgaacttcct gttcaacgtc taattccgct aatactactc
    20041 tggcggttaa taacgagcca ttatgtgagt tgcacgtaat ggctttttta cgcctgctac
    20101 gcgtcttctt tcaattctga gttaccaaaa ctcacaatca gactcctgac aatattaaca
    20161 atgcagcttt tatgtgataa caatcacagt gcattaaccg ttcaagagtt tgtcatgaaa
    20221 aaaacattta tcttcagcct gttagcaatt gtcttcgcga gcctgttaag tgcttgtgtt
    20281 cctcaccatc atcaccgcca caacgatggc ccgcgtggtc ctgcggcttc cggtaagcca
    20341 atgccacctt caaatggtgg gccaaatcat ggcagtcatg gtcgctatta attagcaagt
    20401 catacgcggg agagtgcata ctctcccgct atccacggcg cttaaactga ctcacatcaa
    20461 tgccatgttg cttcattttc cgccacagcg tggtgcggcc gattccaagt aaagccgaca
    20521 tttcctgaat gcgaccgcct gtgacctggg ctgcgttaat aattgcctct ttttcaactt
    20581 ccgcaaatga cagactggtg gaaaggcggg tggcgctgac atcatctgtc gcctgctcgg
    20641 taaacagatg ttccggcaaa tcactgacgc gaatgcgccc attatcacta ctcagagcaa
    20701 gattctcgat gacgctgtaa agttcaaaat cgttgccagg ccatgcacag gaaaccaggc
    20761 gagcgagggc atcgtcatca attttcagcc gcgtagagaa gcgtttttca aggctgcgta
    20821 atttgttatt caccagcgca ggaatgctgc cacgccgcat acgcagtggc gggatggtaa
    20881 tttcaaatgc atgcagcgcg taatacagct ggcgactaaa acgattttgt tccaccagca
    20941 ttgcgaggtc cgcggtcgtt gtggcaatca ctttgacatc aatgggtatt aaacgccgcg
    21001 catccagtcg cgtgataaca ccctgcttga taacctgaag caaagcagac tgtaactcca
    21061 ccgccagata ttcaatcttt tcaagaaaca gcgtgccgcc gtgcgccagt tccagccgac
    21121 tcagacggcc attttcatta tccgtgcgat cgccaccaat aaattcttcc gccagcgccg
    21181 catcaccata taactcacaa ttgacggcga tataaggccc cgctgcacgc tcgctttcat
    21241 tatgaattgc ctgacttagc agtgccttgc ccacgccctc ttctccacaa agcaggacag
    21301 gaaagctact gcgtgccgcc tggcgaccaa aatgaatcaa gcggcgggtt tgcggatcgt
    21361 cctgtggcat atgagcgaag gtatggctga cttttcctaa ttgactggtc attaactgcc
    21421 gcatctgttc cacaggatgg agcaataaaa taaagctggt tccctgcgtt tctattatcg
    21481 gtttaagggt tatcaccgca tcaataaact gatgctgact ttcaaaggtg gcttctacgt
    21541 gtttgagcgg atgtgcctgt tttattgctt gttgcaatac ggcgggtaac gttaagagtt
    21601 cagtgattgc ccttccctga cttgccgtcg cgtcaaggcg caagacccgc gccgcctggg
    21661 cattgataaa ttgcaaatta ccctgctcgt cccagctaat cacgccatca tccatacttt
    21721 ctaacagggc attaagttga tttaaatgac ggttagtttc agcgagcaaa ctgtccgtca
    21781 gcagtaaatt tccgacctcg cgtgcgattg ccaacgtcaa cggcaaatca gctgcggtag
    21841 tttgctcaac cggacacgcc agcgctattg ttcccgtcaa tcggcccttg ctgtcaaaca
    21901 acggcgttgc gcaaaaggcc cagttccaga gtgcctgttt gaaatgttga tcggccatcg
    21961 ttttcacggc ctgaccagag atagccgcta acgatagcgc acaagtacca ataattccct
    22021 cggcgcaata cgtgccgtca ttgaatccca gtgtacttag ttgctgcaag gtttgcggat
    22081 cgccattacg gctgagaatg caggcggttt catcgaggat aagcaacgca cactctcgcg
    22141 gtgccatata ttcccaggca tcttccagcg cagcctgccc gagcgtcagc atcgctttct
    22201 tacgccgata aatagaagca aatgtcacgc cctgggcctg gtgtggtacg ttccatgtct
    22261 cccgtttcat cagcttattg catctctccc aggaggttgc aattaaggga gatatgcccc
    22321 gaccatcgtt gttaaaagcg ccactcatat cccgcatccc ttatgacaaa aggtgttccg
    22381 gctcgcaatt ttaagtatta atacacgtaa aagtttcaat atggaacatt cattaaagca
    22441 tatgtttcac tatgaaacct aatttgggct aattttcttt tcccttgcct gatgcacaat
    22501 ggattcatcg gcagtcagtg gtcgccgtgt cgttgaacat catccatgcc ctaccgtaat
    22561 tgctggagca aaataatgaa aaaattgatc aatgatgtgc aagacgtact ggacgaacaa
    22621 ctggcaggac tagcgaaggc gcatccatcg ctgacactgc atcaggatcc ggtgtatgtc
    22681 acccgagctg atgcccccgt tgcagggaaa gtcgccctgc tgtcgggtgg cggcagcgga
    22741 cacgagccga tgcactgtgg ctatatcggt caggggatgc tttcgggagc ctgtccgggc
    22801 gaaattttca cctcaccgac gcccgataaa atctttgaat gcgccatgca aattgatggc
    22861 ggcgaaggtg tactgttgat tatcaaaaat tacaccggcg atattcttaa cttcgaaaca
    22921 gcaaccgagt tactgcacga tagcggcgta aaagtgacca ctgtggtcat tgatgacgac
    22981 gttgcagtaa aagacagtct ttataccgcc gggcggcgcg gcgttgccaa caccgtatta
    23041 attgaaaaac tcgtaggtgc agcggcggag cgtggcgact cactggacgc ctgtgcggaa
    23101 ctggggcgta agttgaataa tcaaggccat tcaataggta tcgctctcgg tgcctgtacc
    23161 gttcctgccg cgggcaaacc ttcttttacc ctggcggata atgagatgga gtttggcgtc
    23221 ggcattcatg gtgagccagg tattgaccgc cgccccttat cttcccttga tcaaaccgtc
    23281 gatgaaatgt tcgacaccct gctggaaaat ggctcatacc atcgcacttt gcgtttctgg
    23341 gattatcaac aaggcagctg gcaggaagaa ccacaaacca aacaaccgct ccagtctggc
    23401 gatcgggtga ttgcgctggt taacaatctt ggcgcaactc cgctttctga gctgtacggc
    23461 gtctataacc gcctgaccac acgttgccag caagcgggat tgactatcga acgtaattta
    23521 attggcgcgt actgcacctc actggatatg accggtttct caatcacctt actgaaagtt
    23581 gatgacgaaa cgctggcact ctgggacgcc cctgtccaca ccccggccct taactggggt
    23641 aaataaggag aaagcaatgt cactgagcag aactcaaatt gttaactggc tcactcgttg
    23701 tggcgatatt ttcagcaccg agagcgagta tcttactgga ctggatcgcg agattggcga
    23761 cgctgaccac gggctaaata tgaatcgagg ctttagcaaa gtggtggaaa aactccctgc
    23821 tatcgcagat aaagatatcg gtttcattct caagaatacc ggcatgacgc tactttccag
    23881 cgtcggaggt gccagtggtc cgctgttcgg taccttcttt atccgcgccg cacagacaac
    23941 ccaggcacgg caaagcctga cgctggaaga gctttatcag atgttccgcg atggcgcgga
    24001 cggcgtaatc agtcgcggga aagccgaacc tggcgataaa accatgtgtg atgtgtgggt
    24061 gccggtggtg gaatcgttac gtcagtcctg cgagcaaaat ctccctgttc cggcggcgct
    24121 cgacgctgcc agtagcatcg ccgaatccgc tgcacaaagt acgattacga tgcaagcccg
    24181 caaaggccgc gccagttatc tcggtgaacg cagtattggt caccaggatc ccggcgcgac
    24241 ctcggtgatg tttatgatgc aaatgttggc gttagccgca aaagagtaag gaattggtga
    24301 tggtaaacct ggtcatagtt tcacatagca gccgactggg agaaggtgtc ggtgaattag
    24361 cccgtcagat gttaatgagt gataattgta aaatcgccat tgccgcggga attgacgatc
    24421 cacaaaatcc cattggtacc gatgccgtca aagtgatgga ggccatcgaa tctgttgctg
    24481 atgccgacca tgtgctggtc atgatggata tgggtagcgc attattgagt gctgaaactg
    24541 cgctggaatt gctggctccc gagatcgccg caaaagtacg tttgtgtgct gcgccgttgg
    24601 tcgaaggtac actggcagca acggtcagcg cggcgtcggg ggcggatatc gacaaagtca
    24661 tctttaacgc catgcatgcg ctggaagcca aacgtgaaca actgggttta ccgtcctccg
    24721 acactgaaat ctctgacaca tgtcctccgt acgatgaaga agcccgttct ctgtcggtgg
    24781 tcataaaaaa ccgtaacggc ctgcatgtac gtccggcctc ccggctggtt tataccttat
    24841 cgacatttaa tgccgatatg ttgctggaga aaaacggcaa atgcgttaca ccggatagta
    24901 ttaaccagat tgcgttacta caagttcgct ataacgatac gctgcgcctg attgcgaaag
    24961 gaccagaagc tgaagaggca ctgatcgctt tccgtcagct ggctgaagat aactttggtg
    25021 aaacggagga agttgctcca cctactctgc gtcccgttcc gcctgtttcg ggtaaagcct
    25081 tttattatca acctgtttta tgtacggtac aggcaaaatc aaccctgacc gtggaagaag
    25141 aacaagaacg attacgccag gccattgact tcacgttatt agatctgatg acgttaacag
    25201 cgaaagcaga aacctgcggg cttgacgata ttgccgcaat cttttctggt caccatacac
    25261 tgttagatga tccggaactg caggcggcgg caagcgaact ccttcagcat gaacattgca
    25321 cggcggaata tgcctggcag caagttctta aagaacttag ccagcaatac caacagctgg
    25381 atgatgaata tctacaggct cgctatatcg atgtggacga tcttctgcat cgcaccctgg
    25441 tccacctgac ccaaacgaaa gaagaactcc cgcagtttaa ctcgccaact attctactgg
    25501 cggagaacat ttatccctcc acagtactgc aactggatcc agcggttgta aaaggtatct
    25561 gccttagcgc cggaagtccg ctatcccaca gtgccctaat cgcccgtgaa ctgggaattg
    25621 gctggatttg ccagcagggt gagaaactgt atgcgataca accagaagaa acgctaacgc
    25681 tggacgttaa aacgcaacgt ttcaaccgtc agggttaaac caccgttcgg atggcatcat
    25741 tctgatgtca tccggcaatt tactcagcac tgatgttttt aactacctgt tatatttttc
    25801 accaaattat gatgattttt cttcgctaat cgcaaaccca acgtcgctga ctgactaata
    25861 aacagatgtt attttatcac tctatttcgc cgttatttat ccctttaatt cccttttcta
    25921 aaatgtctgt cagttcgcag aatgtgattt cgatcatgca gctagtgcga tcctgaacta
    25981 aggttttctg atacttgaat accgttttat tcggtttcgc caaaggagaa tgactgatga
    26041 aatcccccgc accttctcgc ccgcaaaaaa tggcgttaat tccagcctgc atctttttgt
    26101 gtttcgctgc gctatcggtg caggcagaag aaacatcggt aacaccacaa ccgcctgata
    26161 ttttattagg gccgctgttt aatgatgtgc aaaacgccaa actttttccg gaccaaaaaa
    26221 cctttgccga tgccgtgccg aacagcgatc cgctgatgat ccttgctgat tatcggatgc
    26281 agcaaaacca gagcggattt gatctacgcc atttcgttaa cgtcaatttc accctgccga
    26341 aagagggcga gaaatatgtt ccgccagagg ggcaatcact gcgcgaacat attgacggac
    26401 tttggccggt attaacgcgt tctaccgaaa acaccgaaaa atgggattct ctattaccgc
    26461 tgccaaaacc ttatgtcgtg cccggcggac gttttcgcga ggtatattac tgggacagtt
    26521 acttcaccat gttaggactt gccgaaagcg gtcactggga taaagtcgcg gatatggtag
    26581 ccaattttgc tcatgaaata gacacttacg gtcatattcc caacggcaac cgcagttact
    26641 atttaagccg ctcgcagccg cccttctttg ccctgatggt agagttactg gcgcagcatg
    26701 aaggcgacgc cgcgttgaaa caatacctgc cgcaaatgca aaaagaatat gcttactgga
    26761 tggacggtgt tgaaaacctg caagccggac aacaggaaaa acgcgttgta aaacttcagg
    26821 atggtaccct tctcaaccgc tactgggacg atcgcgatac gccacgacca gagtcatggg
    26881 tggaagatat tgccactgcc aaaagcaatc cgaatcgacc tgccactgaa atttaccgcg
    26941 acctgcgctc tgccgctgcg tctggctggg atttcagctc acgctggatg gacaatccgc
    27001 agcagttaaa taccttacgc accaccagca tcgtaccggt cgatctgaac agcctgatgt
    27061 ttaaaatgga aaaaatcctc gcccgcgcca gcaaagctgc cggagataac gcgatggcaa
    27121 accagtacga aacgctggcg aatgcccgtc aaaaagggat cgaaaaatac ctgtggaacg
    27181 atcaacaagg ctggtatgcc gattacgacc tgaaaagtca taaagtgcgc aatcagttaa
    27241 ccgcggccgc cctgttcccg ctgtacgtca atgcggcagc gaaagatcgc gccagcaaaa
    27301 tggcgacggc gacgaaaaca catctgctgc aacccggcgg cctgaacacc acgtcggtga
    27361 aaagtggaca acaatgggat gcgccaaacg gctgggcacc gttgcagtgg gtcgcgacag
    27421 aaggattaca aaactacggg caaaaagagg tggcgatgga cattagctgg cacttcctga
    27481 ccaatgttca gcacacctat gaccgggaga aaaagctggt ggaaaaatat gatgtcagcg
    27541 ccaccggaac agggggcggc ggtggcgaat atccattaca ggatggcttt ggctggacca
    27601 atggcgtgac gctgaaaatg ctggatttga tctgcccgaa agagcaaccg tgtgacaatg
    27661 ttccggcgac gcgtccactc agtgagtcaa caacacagcc ggtaaaacaa aaagaggcgg
    27721 aacctacgcc ttaactttgc tgtatatcga gcaatgccat tatgtgcata aaattggcat
    27781 tgctcttttt tcgccacgaa atgcc
//
LOCUS       DAZKHK010000070        27293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKHK010000070 DAZKHK010000000
VERSION     DAZKHK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.070"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..321
                     /locus_tag="ACHOS5_002897"
     CDS             136..321
                     /locus_tag="ACHOS5_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777688.1"
                     /translation="MSRRYSLATITLVILFCGRPHCRKISVRQYYQKRVIFYPTTLQR
                     TAFKKCLFLIRDIIISS"
     gene            282..401
                     /locus_tag="ACHOS5_002898"
     CDS             282..401
                     /locus_tag="ACHOS5_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9777689.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDEAPGLVPAD"
     gene            436..778
                     /locus_tag="ACHOS5_002899"
                     /pseudo
     CDS             436..778
                     /locus_tag="ACHOS5_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1152..1418)
                     /gene="minE"
                     /locus_tag="ACHOS5_002900"
     CDS             complement(1152..1418)
                     /gene="minE"
                     /locus_tag="ACHOS5_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP9777690.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1422..2234)
                     /gene="minD"
                     /locus_tag="ACHOS5_002901"
     CDS             complement(1422..2234)
                     /gene="minD"
                     /locus_tag="ACHOS5_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP9777691.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2258..2953)
                     /gene="minC"
                     /locus_tag="ACHOS5_002902"
     CDS             complement(2258..2953)
                     /gene="minC"
                     /locus_tag="ACHOS5_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP9777692.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3473..3841
                     /gene="ycgJ"
                     /locus_tag="ACHOS5_002903"
     CDS             3473..3841
                     /gene="ycgJ"
                     /locus_tag="ACHOS5_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP9777693.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3944..4345)
                     /gene="pliG"
                     /locus_tag="ACHOS5_002904"
     CDS             complement(3944..4345)
                     /gene="pliG"
                     /locus_tag="ACHOS5_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP9777694.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4587..4880
                     /locus_tag="ACHOS5_002905"
     CDS             4587..4880
                     /locus_tag="ACHOS5_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP9777695.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4952..5611
                     /gene="ycgM"
                     /locus_tag="ACHOS5_002906"
     CDS             4952..5611
                     /gene="ycgM"
                     /locus_tag="ACHOS5_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9777696.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5703..6149
                     /gene="ycgN"
                     /locus_tag="ACHOS5_002907"
     CDS             5703..6149
                     /gene="ycgN"
                     /locus_tag="ACHOS5_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP9777697.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6356..6682)
                     /gene="hlyE"
                     /locus_tag="ACHOS5_002908"
                     /pseudo
     CDS             complement(6356..6682)
                     /gene="hlyE"
                     /locus_tag="ACHOS5_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            6942..7361
                     /gene="umuD"
                     /locus_tag="ACHOS5_002909"
     CDS             6942..7361
                     /gene="umuD"
                     /locus_tag="ACHOS5_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9777698.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIIIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7361..8629
                     /gene="umuC"
                     /locus_tag="ACHOS5_002910"
     CDS             7361..8629
                     /gene="umuC"
                     /locus_tag="ACHOS5_002910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP9777699.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANYAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8674..9204)
                     /gene="dsbB"
                     /locus_tag="ACHOS5_002911"
     CDS             complement(8674..9204)
                     /gene="dsbB"
                     /locus_tag="ACHOS5_002911"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP9777700.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9350..10891)
                     /gene="nhaB"
                     /locus_tag="ACHOS5_002912"
     CDS             complement(9350..10891)
                     /gene="nhaB"
                     /locus_tag="ACHOS5_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP9777701.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            11113..11832
                     /gene="fadR"
                     /locus_tag="ACHOS5_002913"
     CDS             11113..11832
                     /gene="fadR"
                     /locus_tag="ACHOS5_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP9777702.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11883..13415)
                     /gene="ycgB"
                     /locus_tag="ACHOS5_002914"
     CDS             complement(11883..13415)
                     /gene="ycgB"
                     /locus_tag="ACHOS5_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP9777703.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13745..15043
                     /gene="dadA"
                     /locus_tag="ACHOS5_002915"
     CDS             13745..15043
                     /gene="dadA"
                     /locus_tag="ACHOS5_002915"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP9777704.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKVLRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     HFNTPVDQLLCDGEQIYGVKCGDEVVKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            15053..16123
                     /gene="dadX"
                     /locus_tag="ACHOS5_002916"
     CDS             15053..16123
                     /gene="dadX"
                     /locus_tag="ACHOS5_002916"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP9777705.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSAIGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(16180..17916)
                     /gene="cvrA"
                     /locus_tag="ACHOS5_002917"
     CDS             complement(16180..17916)
                     /gene="cvrA"
                     /locus_tag="ACHOS5_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP9777706.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(18011..18925)
                     /gene="ldcA"
                     /locus_tag="ACHOS5_002918"
     CDS             complement(18011..18925)
                     /gene="ldcA"
                     /locus_tag="ACHOS5_002918"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP9777707.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLSVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            19025..19636
                     /gene="emtA"
                     /locus_tag="ACHOS5_002919"
     CDS             19025..19636
                     /gene="emtA"
                     /locus_tag="ACHOS5_002919"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP9777708.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(19638..20372)
                     /gene="ycgR"
                     /locus_tag="ACHOS5_002920"
     CDS             complement(19638..20372)
                     /gene="ycgR"
                     /locus_tag="ACHOS5_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9777709.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            20573..20827
                     /gene="ymgE"
                     /locus_tag="ACHOS5_002921"
     CDS             20573..20827
                     /gene="ymgE"
                     /locus_tag="ACHOS5_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9777710.1"
                     /translation="MGIIAWIIFGLIAGVIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20877..22847)
                     /locus_tag="ACHOS5_002922"
     CDS             complement(20877..22847)
                     /locus_tag="ACHOS5_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9777711.1"
                     /translation="MRLKKRYLCTVLSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQISGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYHDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22873..23727)
                     /gene="modD"
                     /locus_tag="ACHOS5_002923"
     CDS             complement(22873..23727)
                     /gene="modD"
                     /locus_tag="ACHOS5_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFP9777712.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLISAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(23724..24536)
                     /locus_tag="ACHOS5_002924"
     CDS             complement(23724..24536)
                     /locus_tag="ACHOS5_002924"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9777713.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVLQETLR"
     gene            complement(24546..25304)
                     /locus_tag="ACHOS5_002925"
     CDS             complement(24546..25304)
                     /locus_tag="ACHOS5_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9777714.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(25301..26281)
                     /locus_tag="ACHOS5_002926"
     CDS             complement(25301..26281)
                     /locus_tag="ACHOS5_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9777715.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(26281..>27293)
                     /locus_tag="ACHOS5_002927"
     CDS             complement(26281..>27293)
                     /locus_tag="ACHOS5_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFP9777716.1"
                     /translation="RRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITRIVSA
                     GAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSLEGLM
                     ALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLGEEHR
                     AAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLGLHNV
                     AQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRILFLSG
                     LPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADYQKLV
                     AS"
BASE COUNT         6848 a         6674 c         7040 g         6731 t
ORIGIN      
        1 ggaatgagaa taatctcaaa tgagaaataa ctcattgatt gacaatattt ttattcaaga
       61 agtgtcattg gctgttaata caatgttgta aaggtaagat aatctgattt atcaatatta
      121 ttgtgtgatt gttatgtgag cagaagatat tcattagcaa cgattacatt agtcatttta
      181 ttttgtggac ggcctcattg tcgaaaaata agcgtacgac agtattatca gaaaagagtg
      241 attttttatc caacaacact tcagcgcact gcgtttaaaa aatgcctctt tcttatacgg
      301 gatatcatca tttcatcatg atgtctttga tgagcggtga acacaataca tttgcgctgt
      361 ctcttcagga tgaagcccct ggtctggtgc ctgcggactg atgttgcagc agagctaagg
      421 tcacttagac gctactatca tttatccaat gcatggaatc gaaatcagtc gatacccgca
      481 gtatatatcg tgaactgggc gcaacgctga gttacaacat gcgtctgggg aacggtatgg
      541 aaattgaacc ctggctgaag gcggctatgc gcaaagaatt tgtcgatgat aaccgggtga
      601 aggtgaatag tgacggtaat tttgtcaatg atttgtcggg cagacgtgga atataccagg
      661 caggtattaa agcctcattc agcagtacgt taagcgggca tctcgggggg ggtataaccg
      721 tggtgccggt gtggagtccc cgtggaatgc ggtggccggt gtgaactggt cgttctgacc
      781 atcaacgatt aaactgcgtt tcggcgctgt ttttgtttac cgggtgctaa aagggaaaat
      841 tctggggcaa aaaaaaagcc cgtcagttac ggcgggaaac ctcatcctat gggagaacaa
      901 tgaataatga aattgcgggg ttatcatctc ccagaatacc catgctaacg ataaggttat
      961 ttactcaacc aggcgtaaac attttgttct gtgcgtggga acagccttaa ggtgtaaagg
     1021 gggaggggga aatagcaatg aatagtatca gcaagaatac tcgccgcttt accacaacgt
     1081 ggatgagagg gatgaaaaac tcaaggcaga gatgactctg ccttgaagat aaatgcgctt
     1141 ttacagcggg cttatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata
     1201 gaaatatcgc catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtaca
     1261 tatttacaaa tgacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca
     1321 tcgctgcgac ggcgttcagc aacaataatc tgcaaccgtt cttttgcaat gttggctgtg
     1381 tttttcttcc gcgagagaaa gaaatcgagt aatgccataa tttatcctcc gaacaagcgt
     1441 ttgaggaagc ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga
     1501 cgttctacgg tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt
     1561 tcaccctggt tagaggcgcg caatactgat tgatcctctg ggatcacgcc gacgagtttg
     1621 atgcgcagga tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct
     1681 gggttatagc gcgttaacag caggtgctct ttaatcggct cttcgccatt ttctgcgcgg
     1741 cgtgatttcg acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc
     1801 gggttggtgg taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca
     1861 atccctgccg gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga
     1921 actttggcga ccccttcacg agtgagggca tctttatcgc gagtttgcga tgccggaaga
     1981 atatagagat tttcagtacg tttatcttta attaacgcct ggtttagcgt tgcatcgccc
     2041 tgaatgacgt tgacgaaatc gtaaacgacc cggcgttcac aacccataat caggtcgaga
     2101 ttacgcaggc cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca
     2161 gtggcgatgg ccgcgctgga ggttgtctta ccaacacccc ctttgcccga agtaacaaca
     2221 ataatgcgtg ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca
     2281 aagcgttttc gactaactgc agtcgtgccg ctttgccata aaattctgct gggatttgat
     2341 cactcagcca gtattcacct gcgatggaca ccagttccgc catcaggttc gtacaaaata
     2401 tttgcgtttc ccggtcacca cttgcccctg ccagcgcacg accgcgcatc atgccataga
     2461 catgaatgtt accatcggca atcaattcgg cccctgcgct aacgtggctt gtaacaatca
     2521 gatcacattg tggagcataa atacgctgac cggaacgcac tggggtatca attaaacgcg
     2581 tttttgtgac cggcgttgta ttttgcgctg gagcctgcgg tgcgggagct ggacgcggcg
     2641 ctttttcctt tccttccgtc aggataggca gccccatctt ttcaatttcg gctttaagtt
     2701 gcgcatcttt gcagccactt acgccaataa cccgcaaacc agttgccgaa accgccttat
     2761 gcatcgccga ccagtttacc gggtcttcca gtgcactgac gttgagtaca acgggggcat
     2821 gttttaaaaa tgcgggggcc tgagcgattt tgtcttccag cgcctgatga ataaccttag
     2881 gttctgcctc atgcagatga accacagata aagtgaagct actgccttta agctcgattg
     2941 gcgtgtttga catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat
     3001 gttccgaaga ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca
     3061 tttattcaga gtaaaagtct attctgtgat aaatggcgct gattcatagc ttaaaaaata
     3121 cccttgtcaa tcaacccatt gccgtcgtac ttttgattgt tcttatttac gcttcctttt
     3181 tccgcaccct aatcgtgaca gcatcttcag acctgtttaa atatccagtg tttaggcgag
     3241 tgtatttttt caccacgcca ggctttctta actttccatt ttttgcagca tcattaaaga
     3301 acagataccc gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag
     3361 acgtctgata cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga
     3421 ttgatgatga aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat
     3481 gatgcgtatt ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc
     3541 tggaaatgac gctggcggac ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat
     3601 ttgtgttaac tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc
     3661 cgctgaaaac ttacaatcag tacaaggtta cgatcccagc gaatttacat tctctaacgg
     3721 tgtattttgc gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtgaatgg
     3781 taagcggagt ggaaaaatca atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg
     3841 atgtcttgtt tacattatgt tctattcgtc attaacctct taagcgacag agaggagtgg
     3901 ggcgttaacg tttatgacgg ggagagtccc cgtcggttga tatttactta atttgaatat
     3961 cgacgttata ttttttagtt ttgtttttac gggcgtcgtt tcgggtttgc agcactcgca
     4021 gctcatattt gccagaagca gggagtgagt attgtccgtg actatcgagt tctggggaat
     4081 atctggataa atcaacagaa tcatcgattc ctggcccgaa gaggtaagta tcagcgcctt
     4141 cattagagat actcacgtgc actttctgac cttttttggc atagaaggta tatgtatcgt
     4201 aatcgtatcc ctttatttcg ccggagtact gagcgctgct atggcctttt ctgaactcaa
     4261 cattgacatt cttacccgca gcaaagctcg tggaggttag cagtgcaagc aacaatacag
     4321 ctttactgat gcccttaatt ttcattggta gtctcctgtt cagaatgagt acgatgaact
     4381 gttataatat aacaatcctt cacgggagaa ttccagtggc tttcctgtgt tcggttaaat
     4441 cccggcgcgg catttttgct ggggggatta cttataaagg gggcatggac tgaacaaacc
     4501 gtagaaatct acagctggct ggcttgtggc gcgggtttca ttgatgttag cttatgccaa
     4561 aacctggcat cttaaagagt aaaagtatgt tttgtgtgat ttatcgaagc agcaagcgtg
     4621 accagaccta tttatatgtc gaaaaaaaag acgatttttc gcgtgttcct gaggaactga
     4681 tgaaaggttt tggtcagcct cagttagcga tgattcttcc gctggatggg cggaagaaac
     4741 tggtcaatgc cgatattgaa aaggttaaac aggcattaac cgagcaaggt tactatttgc
     4801 agttaccgcc accacccgaa gatttgctga agcaacatct ttccgtcatg gggcagaaaa
     4861 cagacgacac gaacaaataa ccgatatccg gcggtggcat tatctttgtc ggcgcgggtt
     4921 ttcatatcca cgataaggtg aggggaacgt tatgtatcaa catcacaact ggcaaggtgc
     4981 gctgctggat tatccggtga gtaaagtagt ctgtgttggc agtaactatg ccaaacatat
     5041 taaagagatg ggcagcgcag tacccgaaga gccagtgctg tttattaaac cagaaacggc
     5101 actgtgcgat ctgcggcagc cattggcgat cccatccgat ttcggttcag ttcatcatga
     5161 agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag gctacggaag agcatgtccg
     5221 caaagccatt gccggttacg gcgtggcgct cgatctgaca ttgcgtgatg ttcagggaaa
     5281 aatgaagaaa gccgggcagc cgtgggaaaa ggccaaagcg tttgataact cttgtccgct
     5341 ttccgggttt attcccgcgg cggagtttac cggcgacccg caaaatacaa cgctgggcct
     5401 gagcgtaaac ggcgagcaac gccagcaagg tacgactgcg gacatgatcc ataaaatcgt
     5461 tccgctgatc gcttatatga gcaagttttt taccctcaag gccggtgacg ttgtgctgac
     5521 aggcacgcct gatggtgtcg gcccgttgca aagcggtgat gagctgacag tcactttcga
     5581 tgggcattct ttgacaactc gcgttttgta atactttttg ccgcctgaaa gcggcggcaa
     5641 aacttgcatc gctgtgccag agtggttata aggtgcgttt taacgtaatg gcggaacacc
     5701 tgatgagcga tgtacctttc tggcaaagta aaaccctgga cgaaatgagc gatgcggaat
     5761 gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca taaactgatg gatgaagaca
     5821 ccgacgaaat ctacttcact aacgtcgcct gtcgccagct caatattaaa acctgtcaat
     5881 gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg cattaaatta acccgtgaaa
     5941 atctgccaac attcgaatgg ctgccaatga cctgcgctta tcgtttgctg gcggaaggta
     6001 aaggtttacc tgcgtggcat ccgctactta ctggttcgaa agcggcaatg catggtgaac
     6061 gtatctctgt gcgtcatatc gcagtaaaag aatcagaagt gattgactgg caggatcata
     6121 tcttaaataa acctgactgg gcgcagtgat aaatttaata gatgttgatg ggggctttct
     6181 taattcacgt ctggatgctc gccatttcgt agccctgttt ttgaaagcgc tctgtcataa
     6241 tgagagttac tcgatacctt attgtcaaca ggtaactctc aatctttaaa aaacgtctgt
     6301 atttttaaat gtgataaact ttataccttg agtacataga gagaataatc gcttatcaga
     6361 cttcagatac ctcaaagagt gtcttcttac cgtgtctttt ctgatactca ttacaggtgt
     6421 taattatttt tttggccgct tcttttagta aagaaagcat taaatcatca taatcaacgt
     6481 agaatctggt tgtttcagtt tccgttttta tctccccgat ggcggctatt tcggtagtta
     6541 atttcaattt ggcggcatcg atatctttat tcgcttgttt aaccgtgtta gacagggcgg
     6601 taaagaaact ctgcacagat tttaacttgt tcttcaattc tggaatcagt ttcccttcaa
     6661 ctacgcccgc agcaacagaa tacagtttct tgatgccgtc atccagtacc ttaatgagaa
     6721 tggctttctg ggcgaatgct tcgattctat tttaaaccag gtgatatttt tcctttctgc
     6781 tggatggacg ctgtcgccag aaggccgcgt gagcacagga taagagaacg aaaaatcagc
     6841 agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg acaagaacag
     6901 actactgtat ataaaaacag tataacttca ggcagattat tatgttgttt atcaagcctg
     6961 cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag tgtggctttc
     7021 cttcaccggc cgcagattac gttgaacagc gcatcgatct gaatcaactg ttgatccagc
     7081 atcccagcgc gacttatttc gtcaaagcaa gtggtgattc tatgattgat ggtgggatta
     7141 gtgacggtga tttactgatt gtcgatagcg ctattaccgc cagccatggt gatattatca
     7201 tcgctgctgt tgacggcgag tttacggtga aaaaattgca actacgcccg acggtacagc
     7261 ttattcctat gaacagtgcg tactcgccca ttaccatcag tagcgaagat acgctggatg
     7321 tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgccc tctgtgatgt
     7381 aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg gtaaaccggt
     7441 ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg caaaggcgct
     7501 gggcgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc gctgtggcgt
     7561 ggtttgcttt agcagcaatt atgagcttta cgcagacatg agtaatcggg tgatgtcgac
     7621 gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat tctgcgatct
     7681 gacaggtgtg cataattgtc gcgatctgac tgattttggc agagaaattc gcgcaacggt
     7741 gctacaacgt acccatctta ctgttggtgt ggggatcgcc cagaccaaaa cgctggctaa
     7801 gcttgccaat tatgcggcaa aaaaatggca gcggcagacg ggcggggtgg tggatttatc
     7861 aaatctggaa cgccagcgta aattaatgtc tgctcttccc gtggatgaag tctgggggat
     7921 tggacggcgg atcagcaaaa aactggacgc gatggggatc aaaaccgttc tcgatttggc
     7981 ggattcagat atccggttta tccgtaaaca ttttaatgtc gtgctcgaaa gaacggtgcg
     8041 tgaactgcgc ggtgaaccct gtttgcaact ggaagagttt gcaccgacga agcaggaaat
     8101 tatctgttcc cgctcgtttg gtgaacgcat cacggattat ccgtcgatgc ggcaggccat
     8161 ttgtagttac gctgcccggg cggcggaaaa acttcgcagc gagcatcaat attgtcggtt
     8221 tatctccacg tttattaaga cgtcaccatt tgcgttcaat gaaccttatt acggcaatag
     8281 cgcatcggta aaactgctga cgcccactca ggacagcagg gatatcatta acgccgctac
     8341 gcgatctctg gatgccatct ggcaagcggg ccatcgctac caaaaagcgg gcgtgatgct
     8401 gggggatttc ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca acgcaccgcg
     8461 ccccgggagt gagcaattga tggcggtaat ggatacgctg aatgctaaag agggcagagg
     8521 aacactctat tttgccgggc aggggatcca gcaacaatgg cagatgaagc gtgccatgct
     8581 ttcaccatgt tatacaacgc gaagttctga tttactgcgg gtcaaataaa catagcggca
     8641 ggaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacaggt cacgtttttt
     8701 cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag cgataaaaat
     8761 accgagcagc cactgcggca tttccagacc taaaaattcc cactgacgct cggcgcaatc
     8821 gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt ccgggaaacg
     8881 agccataaaa tcacaggtcg caaacggcga aggatagagc tgaagcatgg tgtgctcgta
     8941 agttaactgc acaccgcgga acgcactata caaccagata accatcgcta cataacgcag
     9001 cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga ataacgcgca
     9061 gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga accacagcgc
     9121 cgtcagttcc agtgccagag cagtaaacgc catcaacagc catgcgcccc ggccttgtga
     9181 acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga gtgcgcagtt
     9241 taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca attggacaat
     9301 tatcgggcga aaaggcaaac cgggcattta tatgcccggt aagttgttat caaagcgttg
     9361 ctatccagcc catttgcata aaccattcgg ttacaggagc aagcgtaaac tcgacgcaga
     9421 gcagcccgac gagtgtcagg acgagggtgt aaggcagggc catccacacc atgcggccat
     9481 aagagagacg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac gcagcctgac
     9541 cgttcggcgt agcgacggag ggcagattgg taccggtatt aatggcgacc gccagcagct
     9601 catattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg ttgatataaa
     9661 tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa atatagaaca
     9721 acgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc gaaaacagtt
     9781 gttggtcgat aatcaccgcg actactgaga aaaacaccgt caacagtgcg gtgaatggca
     9841 gagattcggt gaaggcttta ccgatagcat gttcatcggt aacaccggtc aatgatgtcg
     9901 ccagaataat gaccgacaaa ccaatcaaac cgacttctgc cagatgcagt gccagcgcag
     9961 tcaccagcca gacgccgata atcgcctgca caatcagacg gattttatcc tgacgggtac
    10021 gctggttgcg gctttgatcg tcaaactgtt gcagcacttc gcggactttc tccggcagcg
    10081 tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac aggccacaaa
    10141 tcagaaccgg gacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa tgccagccag
    10201 ccgctttagc gatgatcagg ttttgtggtt cgcccaccat ggtcatcacg ccgcctaatg
    10261 cggtgccgac accggcatgc atcatcagac tacgcagaaa gccacggaac tgttccagaa
    10321 ccactttgta atgcttgtcg atatgactat cgtcttgcag gtcggtatct tcagtacggg
    10381 aagaggctac gcggtgataa ataccataaa aaccgactgc aacgctgatc accaccgcca
    10441 cgacggttaa ggcatcgagg aacgcggaga ggaacgccgc agccacgcaa aaagagagcg
    10501 acagcagcat tttggagcga atacttaaca gcaaacgggt aaatatgaac agcaacagct
    10561 gtttcataaa atagataccc gccaccataa acatcagtaa cagcaagact tcaagatttg
    10621 ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatact gcttcgatag
    10681 ccaacaaacc accggggagc agcgggtagc atttcagggc catagccaga gtgaaaataa
    10741 attccgctac cagcaaccag ccagcgacga aagggctgat gaggaaaatt aacggattta
    10801 cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc aaaaaattgc
    10861 gccatagtgc gcggccccag gatatctcca tgatggtttc ccttacctta gaaataatca
    10921 atgatgtttt tatgtttaaa cgcaaagctt aacggtcaga caggagtgag gcaagtcttg
    10981 atagtcaagg ggaaagagat gcagaaaatg aagccttgat cccttttttc ttctttttgt
    11041 cttctatcag cgtagttagc cctctggtat gatgagtcca actttgtttt gctgtgttat
    11101 ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga agagtacatt
    11161 attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc agaacgtgaa
    11221 ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca acgtctggca
    11281 cgagatggct ggttgaccat tcaacacggc aagccgacga aggtgaataa tttctgggaa
    11341 acctctggtt taaatatcct tgaaacactg gcgcgactgg accacgaaag tgtgccgcag
    11401 cttattgata atttgctgtc ggtgcgtacc aatatttcaa ctatttttat tcgcaccgcg
    11461 tttcgtcaac atcccgataa agcgcaggaa gtgctggcaa ccgctaatga agtggccgat
    11521 cacgccgacg cctttgccga gctggattac aacattttcc gcggcctggc gtttgcttcc
    11581 ggcaacccga tttacggtct gatccttaac gggatgaaag ggctgtatac gcgcattggt
    11641 cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta tcacaaactg
    11701 tcggcgttgt gcagtgaagg cgcacacgat caggtatatg aaacagtgcg tcgctatggg
    11761 catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga tttagccatt
    11821 cagggacgat aatcccttcc gtttaaaaag caaacccctc aaacgagggg ttttcattgt
    11881 ttttacagat ttcccattct cggcgggcaa cgttccagca actcgacgct gccgtcttcg
    11941 ttttgctgtt ccagcatcac atcaaatccc cacaggcgat gcacatgctt cagcacttct
    12001 ttgcgccccc gatccagcgg tgcgcgatta tgtggaatgt aacgcagtgt cagcgaacgg
    12061 tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact taagttatat
    12121 tgcgacgata accggttacg gatttcccga taaccttctt cattatgaat agcggaaatc
    12181 tccagataat tatgccgatc atcatccagc acggtgaaga agcggaaatc acgcatcact
    12241 ttcggtgaca ggaactggct gataaaactc tcatctttga aatcacgcat cgcgaaatgc
    12301 agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc ttcttccgtt
    12361 ggtgactgac aaatccgttt aatatcctgg aacatggcaa acccgagggc atacggattg
    12421 atgccgctgt accacgggct gttatagggc ggctggaaga ccacattggt gtggctgtgc
    12481 aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg gttaaggatg
    12541 gtgtagtgcc agaaggtcgc ccagccttcg ttcatcacct gagtctgttt ttgcggataa
    12601 aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga ttccagcagt
    12661 ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg atagcggcgc
    12721 gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag catattgacc
    12781 tgactttgca gatactcttc gcgactcttc tgccgggctt tctcttcttg cagcgagatt
    12841 ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca cgagtccaga
    12901 agccgttcta cttcatcaac gccataacgt tcttcgcact cggtaatata tttacgggca
    12961 aaaatcagat aatcgacaat cgaactggcg tcggtccagc tacggaataa gtaattgttt
    13021 ttgaagaaag agttatgccc atagcaggca tgagccatca ccagcgcttg catggtaatg
    13081 gtgttctctt ccatcaggta agcgatacac gggttagagt taatgacgat ttcataggcc
    13141 agtccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt accgaatgac
    13201 cagtgcggat agttaattgg catgccgacg ctggagtagg catccatcat ctgttctgag
    13261 gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc cacccggtct
    13321 atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc gctcaaacgt
    13381 gtggtgtcct tattcataga atcgatcgtc gccatacgcg cacctcattg ttgtcggcgc
    13441 tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg cgtcgccgga
    13501 gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt ctgaacggaa
    13561 ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat attgccgcag
    13621 agtcagggag atgtgagcca gctcaccata aaaaagctgt atgttgaata atattttcaa
    13681 ctgagttatc aagatgtaat tggattatta ttcttttact gtatctaccg ttatcggagt
    13741 ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg cctggtactt
    13801 aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggagccg ccctggaaac
    13861 cagtgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg cggcaccagg
    13921 tgtgccttta aaagcgatta aatggatgtt ccagcgccat gcgccgctgg cggttcgtct
    13981 cgacggtacg cagttccagt tgaaatggat gtggcaaatg ttacgtaact gcgacaccag
    14041 ccactacatg gaaaacaaag ggcggatggt acgtctggcg gaatacagcc gtgattgcct
    14101 gaaagtattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg ggacgctgca
    14161 actgttccgt actgaacaac agtatgaaaa cgcgacccgc gatatcgccg tgctggaaga
    14221 tgctggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg agcccgcgct
    14281 ggcagaagtg gcgcacaaac tgacgggcgg cctgcagtta cccaatgatg aaaccggaga
    14341 ctgccagcta tttacccaga atctggcgcg gatggcggag caggcggggg ttaaattcca
    14401 ctttaatacg cccgttgacc aactgctttg tgacggcgag caaatctacg gcgtgaagtg
    14461 tggcgatgaa gtggttaagg ctgatgcgta tgtgatggcg tttggttctt actcgacggc
    14521 gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact cgctgactat
    14581 tccgattgcg caggaagatg gtgcgccggt atccactatt cttgatgaaa cctacaaaat
    14641 cgccattacc cgtttcgata accgcattcg tgtcggcgga atggcggaaa ttgttggttt
    14701 taacaccgag ctgttgcaac cgcgtcgtga aacgctggag atggtggttc gcgatctcta
    14761 tccacgcggc ggtcatgttg agcaggcgac tttctggact ggcctgcgcc cgatgacgcc
    14821 agacggcacg ccggttgtcg ggcgtacacg ctttaaaaat ctgtggctga ataccggtca
    14881 cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg atctgctctc
    14941 ggggcgcacg ccagcgatcc cgtatgaaga tctaagcgta gcgcgctaca gccgtggatt
    15001 tacgccatca cgcccgggcc atttacatgg cgcacacagc taaggaaacg agatgacccg
    15061 tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca ttgtccgcca
    15121 ggccgcgccg tacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg ggcacggtat
    15181 tgagcgtatc tggagcgcga tcggggctac cgatggcttt gcattgctta acctggaaga
    15241 ggcaataacg ttacgtgagc gcggctggaa aggaccgatc ctgatgctgg aaggattttt
    15301 ccatgctcag gatctggaga tttatgacca gcaccgcctg accacctgcg tacacagcaa
    15361 ctggcagctc aaagcactgc aaaatgcgcg gctaaaagca ccgttggata tttatcttaa
    15421 agtgaacagt ggaatgaatc ggttgggctt ccagcccgat cgcgtgctta ccgtctggca
    15481 gcagttgcgg gcgatggcga atgttggcga aatgaccctg atgtcacatt ttgccgaagc
    15541 ggaacatcct gatggaattt ccggcgcgat ggcgcgtatt gagcaggcgg cggaagggct
    15601 ggagtgtcgg cgttcgttgt ccaattcggc ggcgactctg tggcacccgg aagcgcattt
    15661 tgactgggtt cggcctggca ttattttgta tggcgcttcg ccgtccggtc agtggcgtga
    15721 tatcgccaat accggattac gtccggtgat gacgctaagc agtgagatta ttggtgtcca
    15781 gacgctaaaa gcgggcgagc gtgtgggcta cggcggtcgc tatactgcgc gcgatgaaca
    15841 gcgaatcggc attgtcgccg caggatacgc cgacggttat ccgcgccacg cgcctaccgg
    15901 tacccctgtt ttagtggacg gcgttcgcac catgacggtg gggacagtct cgatggatat
    15961 gctggcggtc gatttaacgc cttgcccgca agcgggtatt ggtacgccag ttgagctgtg
    16021 gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtag gctatgagtt
    16081 gatgtgcgcg ctggcgttac gcgtcccggt tgtgacggtg taacttgtta tcgctggatg
    16141 cgaccagcgt cgtatccagc gttataaaac acattaaact taagattcgg cttcttcctc
    16201 cgctacccga acaccaatct tcaacacttc attatcttct ttctcggcca ccgtccagat
    16261 catcccggca aactctacct ggtcaccgac aaccggtgct gcgcctaaca actgctggac
    16321 gatttcaccc agcgtttgct gcttatcgcg atattctcgc ccgtcttcca gaccatatat
    16381 cagcgccaca tcagcatatt tggcgctggc ttcgagaatg aagtcaccaa agaagcgttg
    16441 atccagcgcg accggcggcg actggctgaa cagtttaccg agcgccggga gatcgcgttc
    16501 ccgaccaatt acacacaaca catcgccttc gcgcagtcgg gtgctgccgg tgggatgaag
    16561 caactggtta tcacgaaaca gtgccgcaat acgcgtctct tttggcatat gcaaatcacg
    16621 cagtgccgcg cctacgcacc atttatcggc actcaattga taaacaaact gctcccacgg
    16681 attttccgga tgaatatcca ggccaacgcg tgacaccgga cgtcccactg gcggaacgac
    16741 cactttggct tttttcgccg cccacgagag tgatgttccc tgcaatagca gtgaaaccag
    16801 gaccacaaag aaggcgacat taaagaacag tcgcgcattc tccagccccg ccatcatcgg
    16861 gaacacagcc agaataatcg gcaccgcgcc gcgtaatcct acccagctga taaacacgcg
    16921 ctcacgcaga ttgaagccac ggaacggtag caatccggca aataccgaaa gaggacgggc
    16981 gaagaaaatc atccatgcgg acaaaatgag cgccggaatg gcaataggca gcagatcgct
    17041 tgggttaacc aatagcccca gcaccaggaa catggcgatt tgcgccagcc aggcgaggcc
    17101 gtcgaaattt tgcaggatgc cgtagcggtt gcgaatcggc cgattaccca gcagaaaacc
    17161 gcacagataa acagccagaa taccgctgcc ttccagcgcc gtggttaaag caaaaattaa
    17221 aataccgccg cttaatgcca gcaatggata taatccggcg ggcagggcga tgcgattaat
    17281 catttgcagc agtaaataac caccgccaag cccgatgaca atcccgaggc caaattgctg
    17341 cagaatatcg accacgaaca tccagctgac actgctttca tgctgctgga tcatcgcaat
    17401 cagggtaatc gtcagaaaga ccgccattgg atcattactg ccggattcaa tttccagcgt
    17461 cgagccaaca cgttcgttaa gccccttacc acccagcaga gaaaagaccg ctgcagcatc
    17521 ggtggagccg acgatagcgc cgattaataa tccttcaatc aaatcaagat taaacagcca
    17581 cgccgccatc atgccggtta aaccagaggt gataagcacg cccagcgtcg ccagcgacag
    17641 tgccggtcct aacgccacac gaaaggaact ggcctgagtg cgcatcccgc cgtcgagcaa
    17701 aataatcgcc agcgccaggt tacttaccat gtaggcgaag ggataattat caaacgggat
    17761 accgccgacg ccatcaaccc ccgccagcat gccgatcgcc agaaaaataa ccagaatggg
    17821 aatgccaaga cgggaagaaa atgaactaag taaaatactg ctggtgacaa ggatggatcc
    17881 taagatgaaa aggctaatta ttgttgtggc atccaacggt cggttactcc tgattacgct
    17941 gtctcttata taaaccctac catattagcg gcagagacag cgttttactt agccctgaag
    18001 cgtgattttt ttacatttta agaacaggat gaccgctaat cgtcaactga gtgccttcct
    18061 gggtgttatt cagaatggca tgtgcgccca acggcagcgt aaccgtgcgt tgttcatgac
    18121 caaaatcgag gccggtaatg agtggaatcg acaggcggga acgcaaaaac gcgtacactg
    18181 actccaggtt gtaacctgcg tcataatcat taggcgtgct gccgctaaag ctaccgagaa
    18241 taatcgcctt ctgacgtggc aaaattccag catgatagag ctgcaacagc atacgttcga
    18301 cgcggaaagg gtgctcgtta atatcttcca gcaccagaat accgttctca atttttggca
    18361 tccacggtgt accaatcagt gaaatcagca tcgcaagatt gcctccccac agcgtgcctt
    18421 cggtctgaca tgtcgggcct tccccttgcc attcaatggt gaacgtttta ttgcgtaacg
    18481 ccagccagaa gtggtgctcg gtaaaggcat ttagttcatc cgcgccaaag tttgctacca
    18541 gcatggggcc gctaaaggtg atgacattgc cttgcgccag aagaccgcac tgaatggcgg
    18601 taaaatcgct atgtccgcaa atgagtaacg ggtcatgttg ttggcgagcc accagtgcct
    18661 gccagtcaat atctgccagt aaacgactgg caccgtaacc gccgcgtaca gacagcacga
    18721 tggtgttggg ggtagtcagt ctggcaagag aattgagatc ttccagacgc tccgtttccg
    18781 tacccgcaaa acgctcacaa cgacgggcaa taacctcgac gttattgacc tgatgccccg
    18841 cgtcggtaag gcgctggata ccacgcagcg cggcgtgctg tttaatgcag taacccgatg
    18901 gggcaattaa gtgaaacaga gacatggcaa ttccttgctg acaacagaaa cgaaatgtat
    18961 atcatgccgc ttaggtgtgc cgttgtcacc tcaacggcga ttccaggcta taaggataga
    19021 agaagtgaaa ttgagatggt ttgccttttt gattgtgtta ttagcgggtt gttcatcaaa
    19081 gcatgactat acgaacccgc cgtggaacgc gaaagttccg gtgcaacgtg cgatgcagtg
    19141 gatgccaata agccagaaag ccggtgcagc ctggggcgtc gatccacaat tgatcacggc
    19201 gattatcgct atcgaatcgg gtggtaatcc gaacgcggtg agtaaatcga atgccattgg
    19261 tttgatgcag ataaaagctt caacctccgg acgtgatgta tatcgtcgta tgggctggag
    19321 cggtgagccg acgaccagcg agctgaaaaa tccggagcgt aatatttcaa tgggggcggc
    19381 ttacctgaat attctggaaa ccggcccgct ggcaggcatt gaagatccga aggtactgca
    19441 atatgcgctg gtggtgtcat acgctaacgg ggcaggtgcg ctgctacgga ctttctcgtc
    19501 agatcggaaa aaggcgatca gcaaaatcaa cgatctggat gctgatgagt tcctcgatca
    19561 cgtagcgcga aatcatcctg cgccgcaggc tccgcgctat atctacaaac ttgagcaggc
    19621 actggacgcg atgtaaatca gtcgcgcaca ttgtccgctt ttttccgggc ttctcgctcg
    19681 agagagaaaa taatccgctg taattgccgc tccaccgtcg ggctgacgtt aagaaaacgg
    19741 aagctcagac ggggagtggt gatggtttca tttttgccat caatcacttt gcgctcgctg
    19801 atggagatta actgggcgtc aaagtgaaaa acaccccatt gccccatgtt gacttcaatc
    19861 tgagcgaagc gcatgccttc gtgtaattcg gcaggctttg ctgtttccag taatgcgccc
    19921 atgccgccta acgacaaatc atacaggcgg aaacgtaacg tactgttatc cgccagtttg
    19981 gtctggcaaa aataaggcgg atggagtggg gcggtgatgc ggaaatatcg gcgtcgttgt
    20041 acaaaccata aggtgggagg cggtacggta ataaatgccg gaagctgcaa gtattcactc
    20101 tgctgtagtt gttcaacagt aaactcgact ttcgcaccct gagtttcggc ggtaatggta
    20161 atgtgctgcg cctttagcac ggcgatgttg tcttcggcct ggctgccgaa atccagcacc
    20221 agtttatccg gagttattgc cagtattttg ctgatcagct gcccgccatt ccagctgata
    20281 cgcaaaggaa ttgcggcttt gtgcaaatcg cgtaacacgc ccaggacggc taacggattt
    20341 tgtttcagga actgctcatg gtaatgactc acgcggaaaa actcctgact gacaaactgt
    20401 ctttggttta tcggtcgcag ttaacaaaac ttaatacaaa tgcgcgaata ttttttacat
    20461 gttgttctta aatcagccga catacgccca gcatcgatcc cgcccttgcc tatacttaga
    20521 gcgttgacgt aagcatttct tgcgtccaat tcatcgaaac gagggcctga acatgggaat
    20581 tattgcctgg attatttttg gcctgatagc aggcgttatc gccaagctaa tcatgccggg
    20641 gcgtgatggt ggtggatttt tcctgacctg tattcttggg atagtcggtg cggtggtcgg
    20701 cggctggctg gcgaccatgt ttggcattgg cggctccatc agcggtttta atttgcacag
    20761 cttcctggtg gcggtggtgg gagctatcct ggtgctgggc gtattccgcc tcctgcgaag
    20821 agaataagat tttcataagg cggatagcga tacagatgcc gctatccgct ttcacatcag
    20881 aacgtatact cgatacctgc ccagaaattg cgaccttctt caataaaccc ttcactgtag
    20941 gcatatttgg tatcaaacag gttattgacc gacgcattga cgctgaagcc atgacctaag
    21001 gtgtaatcgg ctcgaatgtg agtcaccgca aaaccggcgg ctttttgtga accgtcactg
    21061 ttgctgtagc tggaggaacg cgcctcttcc gacagcgtta cgcttaacgg ctcccacggt
    21121 ttgagagtca tccatgcggt cattgtctgc gttggcagat cggttatctt gccgatgtct
    21181 ttgcgtttgg catcagcgtg gatcagggcg tagctcaatc ctacatccag tatattgctg
    21241 attttcccct taataccggc atccagaccg ctgtaatcca ccgtgccgct gttctgattt
    21301 tgaatggtat cggcatcgat attgtgcgag aggatggcat cactcacccg gttatagtaa
    21361 acgctgacct caaatcccca gtcgtgcgtg aaggcaccat tccaggttaa atccaccccg
    21421 cgcgcgcgtt ccggcttgag ctgcgggtta acaatcgcta tctggttata cgcaggtttg
    21481 gacgtggtat agcgttcttt cagcgtcgga aagcgtgtgc ggtcatagta cgaaagcgcc
    21541 agcgtgtctt cattggcaaa gtggtatttc cccatcacct gccagttaaa agctgactga
    21601 ttgttgtcgt cataatgggt gatactgcca tctttctcat gttttttcgc ttctacgcta
    21661 tcgcgccagt catagctgat tccagccacg acatcgacat tatcggcagc agcccattga
    21721 tattcactgg cgagcgacca ggtacgatct tcatagcgat cgtaagcggc gtgcggcgca
    21781 cctttttccc ggtgtacgtc atctttccag ttaacggcaa acgacagcag atcgttttca
    21841 cgcacatcgg ctgccagttg taacccggca ccgtcgctgt aatcggaata atggctgtag
    21901 ctgccttttt tatttttcaa atcagccagc gagttgtaca tcattagcgt attttcaaag
    21961 gtgtcgtgat acagccgact tttcagggta aaacgatcgt ttagttgggt cgttccctga
    22021 taataaaaac tttctttgtc atactctggc cactgccagt agcgtgattt ctgaccacta
    22081 tttccactgt atggcgggtt atctttttca ccatcctgct taatgtaagt caatgtgtat
    22141 tcatcgtttt cgcgtggtgt aaaaccgagc ttcacaatgc cgcgtttatc atcagccgat
    22201 gaattaatca tcttgccgtg tttgcctgca atatcattat ttacaccatg cggcaggccg
    22261 agaaaatcct gctttagctg gctaccgctg atttgcaaat accccagatc gctgctggcg
    22321 gcaaatgaag catgcatatc gtaggcattg tcctggctac ggctccatcc ctggcgatat
    22381 cccagacttg cttccagagg ttttgttggc ttctgggtgg tgatattaat ggctccgccc
    22441 atctgattag gcccctgaag cagcgacgaa taccctttgg aaacttcaac tgcccccaaa
    22501 ttgttggtca gaatccgcgc cagatcgagg ttgccgtcat agggaacata aatgggcaca
    22561 ccgtcgaaat agaccggcac ctgacgacta tcaaagccac gaactttgac ctgttcttcg
    22621 ttgcggctac ctgacttttg cagcaccacg ccggggacga cacttaatgc ctgagcgaca
    22681 ttctgtttat ccagggcctt catggtgggt tgatcgagaa cggtggtcgt cgttgatgat
    22741 accggactgg accatacggt cagcgtgtcg ctctcctgag ccgctacggc ctgctgggta
    22801 aaggcaagtg ataatactgt gcagagataa cgttttttga gtctcataga ataaattccg
    22861 tttaacaaag aattaaatag aggctgcggg ttgcagactg actttaatgt cagcaggtgc
    22921 cgcgtaataa ggcgcggagg taataaaaag gcgaatgccg cagtcgaggt aatttttgag
    22981 tgttgtcaga ttaattccgc cggtcagagc cagcgtgcag tggggagcca gcgacggtgc
    23041 tatttgcgca atttcagttg cctgttgcgg actaaatttg tcgagctgaa gtacgtctgg
    23101 ttgcgcgcgt aacgcggcga ttgcctcttt cggcgtgtcg gcttcaacaa ctattttctt
    23161 ctcgggtgcg tggcgacgta actgattgat tgcgcctgac cagtcctgat tgtcatgaag
    23221 aaaatggcgg tggttggcaa acaataatat ggtttccgca catccggcac gatgaatcag
    23281 tcctccggca gccagaattg cctgcgaggc cagtaagcgg gtgcccggaa ttgcttttcg
    23341 ggtgcaggcg atattgccat cagggtaacg ttcacgaagt aacgccagca tttgagcgag
    23401 ataatcagaa acaccgcaac tccactccag cacattctgg actgccttcc atccctgatg
    23461 aagtgctgcg gcattacctt gcgcactgat tagccgctga cccgcgttcg cttgtgaacc
    23521 gtcgctgacc gcgtcatcaa tagttaatcc cagcgtagtt aacatcttac acgcgacaga
    23581 aattccgctg acgcagccgc cctgacggag aaaaaactct atatagccat gctggtgtcc
    23641 aatatttaat gctcgcgtgg tcaggtcacc gccctggata tcttccagca gtaacgcatc
    23701 gatttgtgcc tgagaaagga aaatcatcgc agtgtctcct gcagtacgct atcccaccag
    23761 atgagcgccc agtcgcggct ggcaggggcg agggcacgag catcttgttg ttgataccag
    23821 cgatataacc gctcacgctc atcgtcgtta agcgcgccca gactccagct gacgttctgc
    23881 tcaaaacgtt cccaggtgct gttatcctgt tggcaatttt gtccacggat aaaatcaacg
    23941 tgagcataaa ttcccatctg atacaggacg ttgagtgcaa agatatagtt gggtaattcg
    24001 atcacttccc gaccagccgc ccgctgaatg gctggagaga cgaacgaggt gctgaccaga
    24061 tgagtcgtat agacgcgaag ccgtgcctga ttattcagtt tgctcattgc ctggcgcata
    24121 tcagccacca gagtggagcg ggaagccacg gcaatatcgc agcgtggtag gtcactccag
    24181 tcctcttccc aggcgcgctg gatccagtgg acattgtctg cctttagcgc ggcggcgcga
    24241 cgggcggcaa cgttcagcat cccctgactg taatcaacgc cataaatcgt cgtgagtttg
    24301 tccgccagcg ccagcgatac ggtacccggt ccgcagccca tatcgaataa ggtttgcgcg
    24361 ccttgcaggt caatttttga ggtcagttgt tgcaggtagc tgtccgtagg cgaggcgcaa
    24421 ttttccgcca ttttttccgc ccgttgatcc cagtgatccg gcgttttttc tgtgcgatgg
    24481 gccagcctga gttgctgaag gtaaaggtct gcaaaatcaa tatcgtcaat gagcatgatt
    24541 tttccttagt gcttaattgc agagaggtga tggtgaatct ggtcggcaga aacccggtac
    24601 agggcagcca gttttttcgt tgttaactgt tcggtcggta agccttgtgt tactcgtcca
    24661 tctggctcta cctggataat gctgtcggca atggcgttgg catggagcgg atgatgagtc
    24721 gacattaaca ttgtcatgcc gttatttttg agctgcgcca gcgtgtccag tagttggatc
    24781 tgatgaccga aatcgaggct ggaggcaggc tcatccagta ataacaagcg cggttgctgg
    24841 acgagtgcac gggcgattaa agccagctga cgttcaccgc cgctaagcgt attccagcgt
    24901 cgtgtcgcca gatgcaaaat gcccagcttt tccagttgct ccgttgcttt caggcgttct
    24961 tgcttaccgg gaacggaaaa agcgcctatg gtgggggcca ggcccatcaa caccatatcc
    25021 agcacactaa aagcaaaaat gccgtcgtgc gcctgcggca cccaggcaat agcctgcgcg
    25081 cgctggcgat ggctgagtgt ctgcacaggg aggctatcca gtaagatctc gccttttaat
    25141 aacggtataa cgccaagaat ggagcgcatg agtgtggttt tgccgcaacc attggctcct
    25201 aacagacaac aaattgttcc ttgcgacagg gtgaaggaca cgtcgcgtag caccggatgg
    25261 cgggagtaac ccagagacgc atgacggacg gcaagcaaag tcactggcga cctccgcgta
    25321 acaacaaaaa caggaaaaac ggtgcaccaa caaaagcggt cagaatgcca agtgggattt
    25381 ctgttgttcc aatgctgcgt gccagcgtat cggtaagcag gagcagaatc gcgccagtac
    25441 acattgccat gggcaacagc tgctgatggt tatgaccggt caacaagcgg gcaatgtgcg
    25501 gaacgaccag gccgacccaa ccaattatgc ccgctattgc taccgtgctg gcggtgatga
    25561 gtgtggcgca gacaatcaat ccaaagcgca gacgtgtcac gttcagtccc agcgaacggg
    25621 cttcgtcgtc ggaaagcgtg agtaaattca tgcgccagcg gagaaaaaac aggggcaagg
    25681 agcctatgag aatgatcggt gcagcatagc agagatcgcg taaggtcacg gtggacagcc
    25741 cccccagcaa ccagaaggta attgacggta actgggtata aggatcggcc agcgttttta
    25801 ttagcgatat ccccgctccg cacaacgttc ctaacgcaat gcctaccagc accagggtta
    25861 agatcggatc gtggcgggtc acccgacgcg taatcaacca gacgcccgcg accaccatca
    25921 gcccgccgca aaatgcgtac agctggatgt aaacaatgga taatccccac aggattgccg
    25981 cgcaggcacc aagtccggca ccggcggaga cgcctaaaat atccggtgac actaagggat
    26041 tacggaacat tccctgatag gtggttccgg cccctgccag cgccgcgccc agaaacagcg
    26101 cagcaagaat acgcggcacg cggatttgcc agaagacaat ttgttcttgc acaggaacat
    26161 tttcttgacg aagaatgagc gccagcagtt gctgcatatc gagatgataa gccccactta
    26221 cggcggcaaa agtgatactg atagctgcca gcagcaaaat acatccgttt acggttctca
    26281 ttagctcgcc accaattttt gatagtcggc gtcactgagt gaacaatgcc agaacagttg
    26341 ggcgtaatgc tgcatgtcac ttttaaactg gcgattgatg gcgggatcca gccaggcgtg
    26401 aagtctgcgt aatcccagaa gacggttgat tcccggcggg gcatccagcc agccaaaggg
    26461 caggccactt aaaaataaga tgcgttgttc cgcaaccgct ttcacgccct gccagagagg
    26521 atcacgacga ataaaatctg cagtaacggc ctcctgaacc agaataatat ccggctgcca
    26581 gcgcaggaga ttttccatgg aaacctgtgt cagaccgtgg cgatcggcta tttgcgcgac
    26641 gttatgcaaa cctaataatt ccgccgcctc ggtatgcaac gaaccctgta agcccgtttc
    26701 cagccctcga ggaccgcgcg cagcataaaa gctgaggtta gcggcgggtg aggtggcgaa
    26761 tgcctgagct tcaccaacga agcggctggc gagattggct tgttctgcgg cgcggtgctc
    26821 ttcgcctaac gttttgccaa gcgttgttaa ctgttctgct gattgttcca gtttcccgtt
    26881 aagcaataac cagggtattt gtgtctgttc gctaacctgc cgtgcctggg agatccaggt
    26941 ttcatccgta ttgccgcaat caacgaccaa atcgggatgt aacgccataa gcccttccag
    27001 cgagagtgtg ctggcgcgcc cggcgagtcg tcccagcctg gggaactggc gaatgtgctc
    27061 tggcaatgga attaatgcct gacgggcaaa atcaaaagag gaaaaaccga ccattttttc
    27121 tggcgcgaca gccagcaata gtaaatcggc gggcgcgcct gcgctgacga tgcgtgtaat
    27181 gttttgggct tcgggaagcg tcagtgaggc ccagaatttg actgattgtg caaaggacgg
    27241 cagatgatgc aggaacaatg tcgacgcaag aatctgtgta aacctccgtc tgg
//
LOCUS       DAZKHK010000071        27073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKHK010000071 DAZKHK010000000
VERSION     DAZKHK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.071"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..4557
                     /gene="sslE"
                     /locus_tag="ACHOS5_002928"
     CDS             <1..4557
                     /gene="sslE"
                     /locus_tag="ACHOS5_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFP9777717.1"
                     /translation="NKKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPSVDSGSGTL
                     PEVKPDPTPTPEPTPEPTPDPEPTPDPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRVT
                     GATCNGESSDGFTFTPGNTVSCVVGSTTIATFNTQSEAARSLRAVDKVSFSLEDAQEL
                     ANSENKKTNAISLVTSSDSCPADAEQLCLTFSSVVDRARFEKLYKQIDLATDNFSKLV
                     NEEVENNAATDKAPSTHTSTVVPVTTEGTKPDLNASFVSANAEQFYQYQPTEIILSEG
                     QLVDSLGNGVAGVDYYTNSGRGVTDENGKFSFSWGETISFGIDTFELGSVRGNKSTIA
                     LTELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGA
                     TLDEGDQNVVLPNEFIEQFKTGQAKEIDTAICAKTNGCNEARWFSLTTRNVNDGQIQG
                     VINKLWGVDTNYQSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNY
                     WLAFGEKRAWDKNELAYITEAPSIVQPENVTRDTATFNLPFISLGQVGDGKLMVIGNP
                     HYNSILRCPNGYSWGGGVNSKGECTLSGDSDDMKHFMQNVLRYLSNDIWQPNTKSIMT
                     VGTNLENVYFKKAGQVLGNSAPFAFHEDFTGITVKQLTSYGDLNPEEIPLLILNGFEY
                     VTQWSGDPYAVPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASSF
                     VRLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRATGIWVYERYPLVEGELPYTIDEKT
                     KEVIWKYQIDNKPDKKPKLEVASWQEEVDGEQVTQYAFIDVAKHPTDESLKAAKDKIF
                     AAFPGLKECKDPDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAAD
                     LGTNIQRLYQHELYFRTKGSKGERLNSVDLERLYQNMSVWLWNDTKYRYEEGKEDELG
                     FKTFTEFLNCYANDAYDGGTKCSAELKKSLVDNNMIYGESSGKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYDLKANGEVKFT
                     VPYGGLIYIKGDSKDDVSANFTFTGVVKAPFYKDGEWKNALNSPAPLGELESDAFVYT
                     TPKKNLEASNFTGGVAEFAKDLDTFASSMNDFYGRNDEDGKHRMFTYKNLTGHKHRFT
                     NDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLNVPGATE
                     VANNVLALYMQDRYLGKMNRVADDITVAPEYLDESNGQAWARGGAGDRLLMYAQLKEW
                     AEKNFDIKKWYPDGKLPAFYSEREGMKGWNLFQLMHRKARGDDVGNSTFGGKNYCAES
                     NGNAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGAAEMSFEGGVSQSAYNTLAS
                     LKLPKPEQGPETINKVTEYSMPAE"
     gene            4713..5522
                     /gene="pppA"
                     /locus_tag="ACHOS5_002929"
     CDS             4713..5522
                     /gene="pppA"
                     /locus_tag="ACHOS5_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFP9777718.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGETPSTQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            5588..5998
                     /gene="gspS2"
                     /locus_tag="ACHOS5_002930"
     CDS             5588..5998
                     /gene="gspS2"
                     /locus_tag="ACHOS5_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFP9777719.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            6016..6975
                     /gene="gspC"
                     /locus_tag="ACHOS5_002931"
     CDS             6016..6975
                     /gene="gspC"
                     /locus_tag="ACHOS5_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9777720.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSSSVNKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAASDEAKQAVAEPVVSAPV
                     ELPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            7005..9065
                     /gene="gspD"
                     /locus_tag="ACHOS5_002932"
     CDS             7005..9065
                     /gene="gspD"
                     /locus_tag="ACHOS5_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP9777721.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLM
                     QFANGTQIPIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            9065..10558
                     /gene="gspE"
                     /locus_tag="ACHOS5_002933"
     CDS             9065..10558
                     /gene="gspE"
                     /locus_tag="ACHOS5_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9777722.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSLELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCS
                     DCRQAAPATDEEKRLMGISDTHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            10558..11781
                     /gene="gspF"
                     /locus_tag="ACHOS5_002934"
     CDS             10558..11781
                     /gene="gspF"
                     /locus_tag="ACHOS5_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9777723.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGLLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDALQASGMYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALADLRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            11798..12253
                     /gene="gspG"
                     /locus_tag="ACHOS5_002935"
     CDS             11798..12253
                     /gene="gspG"
                     /locus_tag="ACHOS5_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9777724.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12257..12820
                     /gene="gspH"
                     /locus_tag="ACHOS5_002936"
     CDS             12257..12820
                     /gene="gspH"
                     /locus_tag="ACHOS5_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9777725.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRYAQFKDRAVIEGKTLGVLIDPPGYQFMQRRHGQWLPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPVTPFTLRFYA
                     SAQNACWAVKLAHDGALSLNQCDEGMP"
     gene            12817..13188
                     /gene="gspI"
                     /locus_tag="ACHOS5_002937"
     CDS             12817..13188
                     /gene="gspI"
                     /locus_tag="ACHOS5_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9777726.1"
                     /translation="MKRGFTLLEVILALAIFALAAMAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNVLLQAVDIEVSLH
                     DDFSPVIQSRRAWFSAVGGQQ"
     gene            13185..13790
                     /gene="gspJ"
                     /locus_tag="ACHOS5_002938"
     CDS             13185..13790
                     /gene="gspJ"
                     /locus_tag="ACHOS5_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9777727.1"
                     /translation="MRRTRTGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQASWSSGQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            13787..14764
                     /gene="gspK"
                     /locus_tag="ACHOS5_002939"
     CDS             13787..14764
                     /gene="gspK"
                     /locus_tag="ACHOS5_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9777728.1"
                     /translation="MITSPPKRGMALVVVLVLLAVIMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14761..15939
                     /gene="gspL"
                     /locus_tag="ACHOS5_002940"
     CDS             14761..15939
                     /gene="gspL"
                     /locus_tag="ACHOS5_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9777729.1"
                     /translation="MSSMLEIFFPLCAADPIRWQRRTPDVEHGIWPDVADEYLQQWLQ
                     TDTIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDIPLSLTVVGIAPEELSAWAGADAERLTVTALPAIITYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            15941..16477
                     /gene="yghD"
                     /locus_tag="ACHOS5_002941"
     CDS             15941..16477
                     /gene="yghD"
                     /locus_tag="ACHOS5_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFP9777730.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSAAPDIIAARKSGTTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            16764..17651
                     /locus_tag="ACHOS5_002942"
     CDS             16764..17651
                     /locus_tag="ACHOS5_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP9777731.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATDSVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTIHSLACELLPEW
                     RAFFDALLENGGKSLWAEN"
     gene            17984..18193
                     /locus_tag="ACHOS5_002943"
     CDS             17984..18193
                     /locus_tag="ACHOS5_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777732.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            18429..19331
                     /locus_tag="ACHOS5_002944"
     CDS             18429..19331
                     /locus_tag="ACHOS5_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777733.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYTERCAQ
                     GMSLSPVHSARQCPVQPTDSAETE"
     gene            19679..21046
                     /gene="dnaB-PI"
                     /locus_tag="ACHOS5_002945"
     CDS             19679..21046
                     /gene="dnaB-PI"
                     /locus_tag="ACHOS5_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFP9777734.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            21046..22779
                     /locus_tag="ACHOS5_002946"
     CDS             21046..22779
                     /locus_tag="ACHOS5_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP9777735.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGEPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTEREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDDAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVRRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            22772..23497
                     /locus_tag="ACHOS5_002947"
     CDS             22772..23497
                     /locus_tag="ACHOS5_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFP9777736.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            23494..24102
                     /locus_tag="ACHOS5_002948"
     CDS             23494..24102
                     /locus_tag="ACHOS5_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFP9777737.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            24117..24536
                     /locus_tag="ACHOS5_002949"
     CDS             24117..24536
                     /locus_tag="ACHOS5_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFP9777738.1"
                     /translation="MAEINFQVLRENDAGELTGVATAGRPVARHFDDGLTLEVNRTCT
                     TGERNANSALYGAVWRAAKDMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAE
                     SSVALKSTRDLVGSGGVPCVLREIRRMSTTVIHIKGE"
     gene            24540..25007
                     /locus_tag="ACHOS5_002950"
     CDS             24540..25007
                     /locus_tag="ACHOS5_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777739.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            25025..26269
                     /locus_tag="ACHOS5_002951"
     CDS             25025..26269
                     /locus_tag="ACHOS5_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFP9777740.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQLVTEDRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSETEPSEKSEGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            26381..26860
                     /locus_tag="ACHOS5_002952"
     CDS             26381..26860
                     /locus_tag="ACHOS5_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9777741.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMFLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
BASE COUNT         6454 a         6607 c         7924 g         6088 t
ORIGIN      
        1 aataagaaat ttaaatataa gaaatcgctt ttagcggcta ttttaagcgc aaccctgtta
       61 gccggttgtg atggtggtgg ttcaggatcg tcctccgata cgccgtctgt agattctgga
      121 tcagggactt tgccggaagt gaaacccgat ccaacaccaa ccccggagcc gacacctgag
      181 ccgacgccgg acccagaacc tacgccggat ccaacacctg atcctgagcc gacaccagaa
      241 ccggagccag aacctgttcc tacgaaaacg ggttatctga ccctgggcgg aagccagcgg
      301 gtaactggtg ctacctgtaa tggtgaatcc agcgatggct ttacctttac gccaggcaat
      361 accgtgagtt gtgtggtggg cagtacgacc attgcaacat tcaacaccca gtcagaagct
      421 gcgcgtagcc tgcgtgcggt tgacaaagtg tcgtttagcc tggaggacgc gcaggagctg
      481 gcgaattctg aaaataagaa aaccaacgcc atctctctgg tgacgtccag cgacagttgc
      541 cccgcagatg cagaacagct ttgtcttact ttctcgtcag tggttgatcg cgcgcgattt
      601 gaaaaactgt ataagcaaat tgatctggca acagacaatt tcagcaagct ggtcaatgaa
      661 gaggtggaaa acaatgctgc gactgataaa gcgccgtcca cccatacctc aacggtagtg
      721 ccagtcacga cagagggaac aaaaccggat ctgaacgcgt ccttcgtgtc ggctaacgcg
      781 gaacagtttt atcagtatca acccactgaa atcattcttt ccgaaggcca actggtggat
      841 agcctgggga acggtgttgc tggcgttgac tactacacca attcaggccg tggcgtaact
      901 gacgaaaacg gtaaattctc ctttagctgg ggcgaaacca tctcctttgg tatcgatacc
      961 tttgaactgg gctcagtacg tggcaataag tcgaccattg cgctgactga attgggtgat
     1021 gaagttcgcg gggcaaatat cgatcagctc attcatcgtt attcgacgac tggtcaaaat
     1081 aatactcgtg ttgttccgga cgatgtacgc aaggtctttg ccgaatatcc caacgtgatc
     1141 aacgagataa tcaatctttc gttatccaac ggtgcgacgc tggatgaagg cgatcaaaac
     1201 gttgtgctgc ctaacgaatt tatcgagcag tttaagacgg gtcaggccaa agagatcgat
     1261 accgcgattt gtgcgaaaac caacggttgc aacgaggctc gctggttctc gctgacaacg
     1321 cgcaatgtta atgacggcca gattcagggc gttatcaaca agctgtgggg cgtggatacg
     1381 aactatcagt ctgtcagcaa gttccacgtc ttccatgact ctaccaactt ctatggcagc
     1441 accggtaacg cgcgcggtca ggcggtggtg aatatctcca acgcggcatt cccgattctg
     1501 atggcgcgta atgataaaaa ctactggctg gccttcggtg aaaaacgcgc ctgggataaa
     1561 aatgagctgg cgtacattac ggaagcgcct tccattgtgc agccagagaa cgttacgcgc
     1621 gataccgcga ccttcaacct gccgtttatt tcgctggggc aagtcggcga cggcaaactg
     1681 atggttatcg gtaacccgca ctacaacagc attttgcgtt gccctaatgg ttacagttgg
     1741 ggcggtggtg ttaatagtaa aggtgagtgt acgctcagcg gtgattctga tgacatgaag
     1801 cactttatgc agaacgtact gcgctacttg tcaaatgaca tctggcagcc aaataccaag
     1861 agcatcatga ctgttggcac caacctggag aacgtttatt tcaaaaaagc gggccaggta
     1921 ttgggaaata gtgcaccatt tgctttccat gaggatttca ctggtatcac ggttaaacag
     1981 ttgaccagct atggcgatct gaatccggaa gagattccgt tgctgatcct caacggcttt
     2041 gaatatgtga ctcagtggtc tggcgatccc tatgctgtgc ctctgcgtgc agataccagc
     2101 aaaccgaagc tgacccagca ggatgtgacc gatctgatcg cctatctgaa caaaggtggc
     2161 tcggtgctga tcatggaaaa cgtgatgagc aatcttaagg aagagagcgc gtccagtttt
     2221 gtgcgtctgc tggatgccgc gggtctgtca atggctctga acaaatcggt ggtgaacaac
     2281 gatccgcaag ggtatccgga tcgcgttcgt cagcgtcgag cgactggcat ttgggtttat
     2341 gaacgttatc cgctggtaga aggtgaactg ccgtacacca ttgatgaaaa aacgaaagaa
     2401 gttatctgga aatatcagat tgataacaaa ccagataaga aaccgaaact ggaagttgcc
     2461 agctggcaag aagaggttga tggtgaacaa gtaactcaat acgcgtttat tgatgttgct
     2521 aaacatccaa ctgatgagtc tctgaaagcc gcaaaagata agatctttgc agcgttcccg
     2581 gggctgaaag agtgtaagga tcctgactac cactacgagg tcaactgtct ggaatatcgt
     2641 cctggcacgg gggttccggt tactggtggc atgtatgttc cacagtatac gcaactaagc
     2701 cttaatgccg acactgcgaa agcgatggtg caggcggcgg atttaggcac caacattcag
     2761 cgcctgtatc agcatgagct ttatttccgt accaaaggca gtaaaggtga gcgtctgaac
     2821 agtgttgatc tggaacgtct gtaccagaac atgtcggtct ggctgtggaa cgatacgaaa
     2881 tatcgttacg aagagggcaa ggaagatgag ctgggcttta aaacgttcac cgagttcctg
     2941 aactgctacg ccaacgatgc ttacgatggg ggcaccaagt gctccgcgga actgaagaaa
     3001 tcgctggtcg ataacaacat gatctacggt gaaagtagcg gcaaagcggg catgatgaac
     3061 ccgagctatc cgctcaacta tatggaaaaa ccgctgacgc gcctgatgct gggccgttcc
     3121 tggtgggatc tgaacattaa ggttgatgtg gagaagtatc cgggagcagt atcggaagag
     3181 ggacagaacg ttactgaaac catcagcctg tactcgaatc cgaccaaatg gtttgcgggt
     3241 aacatgcagt ctactggtct gtgggctccg gctcagaaag aggtcaccat taagtccaat
     3301 gcgaacgttc ctgtgaccgt caccgtggcg ctggctgacg acctgaccgg acgtgagaag
     3361 catgaagttg cgctgaaccg tccgccaaga gtgactaaaa cctatgatct gaaagccaat
     3421 ggtgaggtga agtttacggt tccttacggt ggtctgattt atatcaaggg cgacagtaag
     3481 gatgatgttt ctgctaactt cacctttacc ggtgtagtaa aagcgccgtt ctataaagac
     3541 ggcgaatgga aaaacgctct gaactcccct gcgccgctgg gcgagctgga atcagacgct
     3601 ttcgtttata ccacgccgaa gaagaacctt gaggccagca atttcactgg tggtgtagca
     3661 gaattcgcta aagatctgga tacctttgcc agctcgatga atgacttcta cggtcgtaat
     3721 gatgaagacg gtaagcaccg gatgtttacc tataaaaact tgacggggca caagcatcgt
     3781 ttcaccaacg atgtgcagat ctccatcggt gatgcgcact cgggttatcc ggtaatgaac
     3841 agcagcttct cgacgaacag caccacgctg ccgacgacgc cgctgaacga ctggctgatt
     3901 tggcacgaag tcggtcataa cgctgcagaa acaccgctga acgtaccggg tgcaactgaa
     3961 gtggcgaaca acgtgctggc gctgtacatg caggatcgct atctcggtaa gatgaaccgt
     4021 gtcgctgacg acattaccgt cgcgccggaa tatctggacg agagcaacgg tcaggcctgg
     4081 gcgcgcggcg gtgcgggtga ccgtctgctg atgtacgcac agctgaaaga gtgggcagag
     4141 aaaaactttg atatcaagaa gtggtatcca gatggcaagc tgcctgcgtt ctacagcgag
     4201 cgtgaaggga tgaaaggctg gaacctgttc cagttgatgc atcgtaaagc acgcggcgat
     4261 gatgttggta acagcacctt tggtggcaag aattactgtg ctgaatccaa cggtaacgct
     4321 gccgacacgc tgatgctgtg tgcctcctgg gtcgctcaga cggatctttc ggagttcttt
     4381 aagaaatgga atccgggtgc gaatgcttac cagctaccgg gagcggcaga gatgagcttc
     4441 gagggcggtg tgagccagtc ggcttacaac acgctcgcgt cgctcaagct gccgaaaccg
     4501 gaacaggggc cggaaaccat taataaggtt accgaatatt cgatgcctgc tgaataacct
     4561 ggataaggcg ttcacgccgc atccggcatg atataaggcg catcgggtca acacatatgc
     4621 ccgatgcgtc agtttatcgg gcctggagga aagccacatg gcatacaccc gcaggcccgc
     4681 atccggcaag ttacaacaaa caacctttaa ccatgctttt tgatgttttt cagcaatacc
     4741 ccgcggcgat gcccatactg gcaaccgtcg gaggattgat catcggcagt tttttgaatg
     4801 tggtgatttg gcgttacccc atcatgctgc gccaacaaat ggcggagttt catggtgaaa
     4861 cgccgagtac gcagtcaaaa ataagccttg cgctaccgcg ttcgcactgt ccgcattgcc
     4921 agcagactat ccgcgttcgt gacaatattc cgctgctctc ctggctgatg ctcaaagggc
     4981 gctgccgcga ctgtcaggcg aaaatcagca agcgttatcc gctggttgag ttattgacag
     5041 cactcgcttt tttgctggcg agtctggtgt ggccagaaag tggatggggg ctggcggtga
     5101 tgatattatc cgcgtggctg attgccgcga gcatcatcga cctcgacaac caatggctgc
     5161 ccgatgtttt tactcagggc gtattgtgga caggactgat tgcggcatgg gcgcagcaga
     5221 gtccgttaac gctacaagac gcagtcaccg gcgttctggt ggggtttatc actttttact
     5281 ccctgcgctg gatagccgga atagttctgc gtaaagaagc attaggcatg ggcgatgtat
     5341 tactcttcgc tgcgttaggt ggatgggtgg ggccgttgtc gttacccaat gtggctttaa
     5401 tcgcatcatg ctgcggtctg atatatgccg ttattacaaa aagaggatca accacactgc
     5461 cttttggacc gtgtttaagt ctgggcggta tagcaacact ttatctacag gcattgtttt
     5521 aatgataacc acgtcattat caaagtgaca ttttaactct tattaataac cttagagatt
     5581 atttaccatg tcgataaaac agatgccagg gagggtatta atatcgctat tgttgagcgt
     5641 tacaggatta ttaagtggct gcgccagcca taatgaaaac gccagtttac tggcgaaaaa
     5701 acaggcgcaa aatatcagcc aaaacctgcc gattaaatct gcgggatata ccttagtgct
     5761 ggcgcaaagt agtggcacga cggtaaaaat gaccattatc agcgaatcgg gtactcagac
     5821 cacgcagaca cctgacgcct ttttaaccag ctatcaacga caaatgtgcg ctgacccaac
     5881 ggtgaaatta atgatcaccg agggaattaa ttacagcata acgattaatg atacacgtac
     5941 aggtaaccag tatcagcgga aactggatcg taccacctgt ggaatagtca aagcataacg
     6001 tcgggtagat ataaattggc gcgggttgtt tttcgtgacg cacgaattta tctcattcaa
     6061 tggctgacta aaattcgtca cattcttagc cagagacaat ctcttaatac agacaaagag
     6121 catctgcgaa aaatggtgcg cgggatgttc tggctcatgc tgcttattat ttctgcaaaa
     6181 atggcgtatt cactctggcg ctatttctcc ttttctgcgg aatatacggc ggtttcctca
     6241 tcggtgaata aaccgctccg tgcggatgca aaaccgttcg ataaaaatga cgtgcaatta
     6301 gtcagccaac aaaactggtt tggcaaatat caacccgtcg ccgcaccggt aaaacaacct
     6361 gaatctgcgc ctgtggcaga aacgcgtctt aatgtggtgc tgcgtgggat cgcctttggt
     6421 gccagacccg gcgcggtgat tgaagaaggc ggcaaacagc aggtctattt gcagggtgaa
     6481 cggcttggct ctcacaacgc agtgattgag gaaatcaacc gcgaccatgt gatgctgcgc
     6541 tatcagggga aaatggaacg tctgagtctg gcagaggagg aacgcccccc tgtggcggtg
     6601 accagcaaaa aagccgccag tgacgaagca aagcaagctg ttgctgaacc tgtcgtcagt
     6661 gcgccagtgg agctcccggc tgccgtgcgt caggcactgg cgaaagatcc gcagaaaatt
     6721 tttaactata tccagcttac gcctgtgcgt aaggagggga ttgtcggtta tgcagtgaaa
     6781 ccgggggcag atcgttctct gttcgatgcc agcggtttta aggaaggcga tatcgccatt
     6841 gcgctaaatc agcaggattt cactgatcca cgagcaatga ttgctctgat gcggcagtta
     6901 ccttcaatgg attccattca acttacggtt ttacgcaagg gtgcgcgcta cgacatttcc
     6961 atcgcgctgc gctaaccgca tttaatccag gagaatcatt catcgtgttt tggcgtgata
     7021 tgacgttgtc ggtctggcgt aagaagacaa ctggcctcaa aacaaaaaag cgtttactgc
     7081 cgctggtgct ggcagcggca ttatgcagtt caccggtctg ggcggaagaa gccactttca
     7141 ccgctaattt taaagatact gacctgaaat cgttcatcga aaccgtcggc gctaacctta
     7201 ataaaaccat cattatgggg ccgggcgttc aggggaaagt gagtattcgc actatgactc
     7261 cgctcaatga acgccagtat taccagttat tccttaacct gctggaagca caggggtatg
     7321 cggtcgtacc gatggaaaac gacgtgctga aggtggtgaa atcaagcgcc gcgaaagtcg
     7381 agccgctgcc gctggtcggt gaaggcagcg acaactacgc gggcgatgaa atggtcacca
     7441 aagtcgtgcc ggtacgcaat gtgtcggtac gcgagctggc accgattctg cgccagatga
     7501 ttgacagcgc aggctcaggc aacgttgtta attacgatcc ctccaacgtg attatgctta
     7561 ccggacgcgc ctccgttgtg gagcgcctga cggaagtgat ccagcgcgtg gatcacgcgg
     7621 gtaatcgcac cgaagaggtg atcccgctgg ataacgcctc agcctcggaa attgcccgcg
     7681 tgctggaaag cctgaccaaa aacagcggcg agaaccagcc agcaacgctg aaatctcaaa
     7741 ttgtcgccga tgaacgcacc aacagcgtga ttgtcagtgg tgaccccgcc acgcgagaca
     7801 aaatgcgccg tctgatccgt cggctggatt cagaaatgga gcgcagcggc aacagccagg
     7861 tgttctatct caaatacagc aaagccgaag atctggtcga tgtgctgaag caggtcagcg
     7921 gtacgctcac ggcggctaaa gaagaggcgg aaggcacggt tggtagcggg cgtgaggttg
     7981 tctccatcgc cgccagcaaa cacagtaatg ccctgattgt tactgcgccg caggacatta
     8041 tgcagtcgct gcaaagcgtg attgaacaac tggatattcg ccgtgctcag gtgcatgtcg
     8101 aggcgttaat cgtggaagtt gccgaaggca gcaatatcaa cttcggcgtg cagtgggggt
     8161 caaaagatgc cggattaatg cagtttgcca acggtacgca aatccctatt ggtacgctgg
     8221 gggcagcgat ttctgcagcc aagccgcaga aaggttccac ggtgatcagc gaaaacggtg
     8281 ctaccaccat aaatccggat actaacggcg atctctccac gctcgctcag cttctttctg
     8341 gctttagcgg tacggcggtt ggcgtggtga aaggcgactg gatggcgctg gtacaggcgg
     8401 tcaaaaacga ctccagctcg aacgtgctct ccacgccgag tatcaccacg ctggacaacc
     8461 aggaagcctt cttcatggtg ggccaggacg ttccggtatt aaccggctct accgttggct
     8521 ccaataacag caatcctttc aatacggtag agaggaaaaa agtcggcatc atgctgaaag
     8581 tcacgccgca gattaacgaa ggtaacgcgg tacagatggt gattgagcag gaagtctcga
     8641 aagtggaagg gcagaccagc ctcgatgtcg tgtttggcga gcgcaaactg aaaaccaccg
     8701 tgctggctaa cgatggtgag ctgatcgtgc ttggcggtct gatggacgac caggcgggag
     8761 aaagcgtggc gaaagtgccg ctgctgggtg atatcccgtt gattggtaac ctgtttaaat
     8821 cgacggcgga taaaaaagaa aaacgtaacc tgatggtgtt tatccgcccg accattttgc
     8881 gtgacggtat ggcggcagac ggcgtgtcgc agcgcaaata taactacatg cgcgctgagc
     8941 aaatctaccg cgatgagcaa ggcttaagcc tgatgccgca caccgcgcag ccgatattgc
     9001 cagcgcaaaa tcaggcctta ccgccggaag tacgcgcgtt cctcaatgcc gggagaacgc
     9061 gttaatggtg cctgtagcac aggaaaccac cgctaacacc gtgcgtctgc cctacagttt
     9121 cagccgtcgg tttagcctgg tggcatggtg cgaagcgtcg ctggagatcc tccatgttca
     9181 tccgttgtcg ctctctgttt tgcaggagct acaacggggg ctgaacgcgc cctttacgct
     9241 gcggcaaatc gacgaggccg aatttgaaca gcggctgaat gcggtctggc agcgggactc
     9301 ttccgaagct cgccagctga tggaagatct cggttccgcc gaggactttt ttaccctcgc
     9361 tgaagaactg ccggaaacgg aagatctgct ggaaagtgac gacgatgcgc cgatcatcaa
     9421 actgatcaac gccatgctgg cagaggcaat caaagaaggc gcttcggata tccacattga
     9481 gacgtttgaa aagagtctgg tgatccgttt tcgtgttgac ggcacattac atgaaatgtt
     9541 gcgccccggt cgtaaactgg cctcgctact ggtatcgcgt atcaaggtga tggcgcgact
     9601 ggatatcgcc gaaaagcgcg taccgcagga tggccgtatt gcgctgctgc tgggcggtcg
     9661 ggcgattgac gtccgtgtat caaccatgcc ttccgcctgg ggggaacggg tggtgctgcg
     9721 actgctggac aaaaaccagg ctcgcctgac gctggagcgt ctgggtttaa gtctcgaact
     9781 gactgcgcag ttgcgccagc tgttacacaa accgcacggc atttttctgg tgacggggcc
     9841 gaccggttcc ggcaaaagca ccacgctgta cgctggattg caggagctga acaaccactc
     9901 gcgtaacatt ctcacggttg aagaccctat cgaatacatg attgaaggga tcggtcagac
     9961 gcaggttaac acccgcgtcg gcatgacatt cgcccgtggc ctgcgcgcaa ttttgcgtca
    10021 ggacccggat gtggtgatgg tcggtgaaat ccgcgatacc gaaaccgcag aaatcgctgt
    10081 tcaggcttca ctgaccggac acctggtact ttccacgctg cataccaaca cagcggtggg
    10141 ggcgatcaca cgtttgcagg atatgggcgt ggagcctttc ctgctctctt ccagtctgac
    10201 gggcgtgatg gcgcagcgac tggtccgcac gctgtgctcc gactgccgtc aggccgcgcc
    10261 tgccaccgac gaagaaaaac gtcttatggg gatctccgat acgcatgccg tcacgctgta
    10321 ccatccgcag ggctgccctg cctgtaatca caaaggtttt cgcggacgta ctgccatcca
    10381 tgaactgatt gtggtggatg ccacattgcg tgatttgatc caccgtcagg ccggggaact
    10441 ggagctggaa cgttatgtcc ggcaacactc tgcgggtatc cgcagtaacg gcattgagaa
    10501 agtgctcgcc ggagaaacct ctctcgatga agttctgcgg gtaaccatgg aggcgtaatg
    10561 gcactgtttt actatcaggc gctggagcgt aatggtcgca aaaccaaagg catgattgag
    10621 gcggattccg cgcgtcatgc ccgtcaattg ttacgcggta aagacctcat tcccgtgcac
    10681 attgaagccc ggatgaatgc atcggcaggg ggattgttgc agcgtcggcg gcacgcacat
    10741 cgtcgcgtgg cggcggcaga tctggcgctg ttcacgcgcc aactggcaac gctggtgcag
    10801 gcagcaatgc cgctggaaac ctgcttacag gcggtcagtg agcaaagcga aaaactgcat
    10861 gtaaaaagcc tcggaatggc gctgcgcagc cggattcagg aaggttatac cctgtcggac
    10921 agcctgcgcg aacatccccg cgtctttgac tccctgtttt gttcgatggt cgctgccgga
    10981 gaaaaatccg gacatctcga cgtggtgctc aatcgcctgg cggattacac cgaacagcgg
    11041 cagcgtctga aatcacgcct gctgcaggcc atgctctatc cgctggttct gctggtggtg
    11101 gcaacgggcg tagtcactat tttgctgacg gcagtggtgc cgaaaattat cgaacagttt
    11161 gatcatctcg gacacgcgct acccgcctcc acccgaatgc tcatcgctat gagcgacgcg
    11221 ttacaggcca gcggcatgta ctggctggcg ggtttgctgg ggcttctggt gctggggcaa
    11281 cggttactca aaaatcctgc gatgcgcctg cgctgggata aaaccttgct gcgcctgccc
    11341 gtgacggggc gtgttgcgcg cggactgaat acggcgcgtt tttcccgcac gttaagcatc
    11401 ctcaccgcca gcagtgttcc gctgttggaa ggcattcaga ccgccgccgc cgtgtcggca
    11461 aatcgttatg tcgagcaaca actgctgctg gcggcagatc gcgtccgcga aggaagcagc
    11521 ctgcgcgccg cgctggcgga tctgcgcctg tttccaccga tgatgctgta catgatcgcc
    11581 tccggcgaac agagcggcga gctggaaacc atgcttgaac aggccgcggt caaccaggaa
    11641 cgggaatttg atacacaggt ggggctggcg ttagggctgt ttgagccggc gctggtggtg
    11701 atgatggcgg gtgtggtgct gtttatcgtc atcgccatcc tcgaaccgat gctgcaactg
    11761 aacaatatgg ttggaatgta atttacggag ttatcacatg aattcgttat cccgcacaca
    11821 aaaaccacgg gcaggtttta ccctgctgga agtgatggtg gtgattgtta ttcttggcgt
    11881 cctggcaagt ctggtggtgc ctaacctgct gggcaacaaa gagaaagccg atcggcaaaa
    11941 agccatcagc gatatcgtgg cgctggagaa tgcgctggat atgtaccgac tggataacgg
    12001 gcgttatccg actaccgaac aggggctgga ggcgctgatc cagcaaccgg ccaatatggc
    12061 ggattcccgt aactaccgta ccggtggata cattaaacga ctgccaaagg atccgtgggg
    12121 caatgattat cagtatctca gcccgggtga aaaagggctg tttgatgttt ataccctggg
    12181 ggcagatggt caggaaaatg gggagggcgc tggcgcagat atcggtaact ggaatttgca
    12241 ggagtttcag taatcagtgc ctgaacgcgg attcactctt ctggaaatca tgctggtgat
    12301 tttccttatc ggccttgcca gtgcgggcgt ggtacagacg tttgcgaccg cttcagagcc
    12361 gcctgcgaaa aaagcggcgc aggattttct gactcgctat gcgcagttta aggacagggc
    12421 tgtgatcgaa gggaaaacac tcggtgtgct aatcgaccca ccaggttacc agtttatgca
    12481 gcgtcgtcac ggacagtggt tgcccgtttc ctcgacccgg ttatcggcac aggttacggt
    12541 gccgaaacag gtgcagatgc tgttacaacc cggaagtgat atctggcaga aggaatatgc
    12601 gctggagctg cagcgtcgcc gcctgacgct gcatgatatt gaactggagc tgcaaaaaga
    12661 ggcgaaaaag aagacaccgc agatccgttt ttcgcctttt gaacctgtca cgccgtttac
    12721 actgcgtttt tacgcgtcgg cacaaaacgc gtgttgggcg gtaaagctgg cgcacgatgg
    12781 cgcgttatct ctcaatcaat gtgatgaggg gatgccatga agcgtggatt taccttgctg
    12841 gaagtaatac tcgcgctggc gatttttgcg ttggccgcca tggcggtgtt acagattgcc
    12901 agcggtgcgc tgagtaatca gcaaatcctt gaggagaaaa cggtggcggg ctgggtagct
    12961 gaaaaccaga ccgcgctgct ttacctgatg acccgtgaac aacgagcggt caggcaccag
    13021 ggcgagagtg atatggcagg aagtcgctgg tactggcgaa cgatccctct gaataccggt
    13081 aacgtgctgc tccaggcggt ggatattgaa gtcagtcttc atgacgactt ttcgccagtg
    13141 attcagtcac gacgcgcctg gtttagcgcc gtgggaggcc aacagtgaga aggactcgca
    13201 ctggtttcac gttactggaa atgctggtgg caatagccat ttttgcctca ctggcactga
    13261 tggcgcagca ggtgacaaac ggcgttacac gcgtgaatag cgccgtcgcc ggacacgatc
    13321 aaaaactaaa cctcatgcag caaacgatga gttttttgaa ccacgatctg acccaaatga
    13381 tgccgcgtcc ggtaagaggc gatcagggcc agcgtgaacc agcgttactg gcgggcgctg
    13441 gcgtgctggc gtctgagagt gaagggatgc gttttgtacg tggcggcgtg gttaatccgt
    13501 tgatgcgtct gccgcgcagt aatctgctca ccgtcggtta ccgcattcat ggtggttacc
    13561 ttgaacggtt agcctggccg ctgaccgatg cagcgggcag cgtgaagcca acaacgcaaa
    13621 aattaattcc ggcggattcg ctccgtttgc agttctacga cggcacgcgc tggcaggcga
    13681 gttggtcgtc agggcaggcg atccctgtgg cggtgcgcat gactctgcat tcgccgcaat
    13741 ggggcgagat tgaacgcatc tggttgttac gcgggccgca attatcatga tcacctcacc
    13801 accaaaacgc ggaatggcac tggtcgtggt gctggtattg ctggcggtca taatgctggt
    13861 aaccatcacg ctttccgggc ggatgcagca acaacttggg cgaacgcgca gccagcagga
    13921 gtaccagcag gcgctgtggt acagcgccag tgcagaaagc ctggcgctga gcgcgctcag
    13981 tctgagtctg aaaaatgaaa agcgtgtgca tctggcacaa ccgtgggctt ctggcccgcg
    14041 ttttttccca ctgccgcagg ggcaaattgc cgtcactctg cgtgacgcac aggcctgctt
    14101 taacctgaat gccctcgctc agccgacgac ggcgtcgcgt ccgctcgcgg tacaacaact
    14161 gattgccctg atctcgcgcc tcgatgtgcc tgcttatcgg gccgaactga tagccgaaag
    14221 cctgtgggag tttattgacg aagaccgcag cgtgcagacg cgtctgggtc gtgaagacag
    14281 cgagtatctc gcccgctcgg tgccgttcta cgccgctaat caaccgctgg ctgatatcag
    14341 cgagatgcgc gtggtgcagg gaatggacgc cgggctttat caaaaactga aaccgttggt
    14401 ctgtgcgctg ccgatggccc gccagcaaat caacatcaat acattagatg tcacgcaaag
    14461 tgtgattctt gaggcgctgt tcgacccgtg gttaagccct gttcaggcgc gagcattatt
    14521 acaacaacgt ccggcgaagg gctgggaaga tgtcgatcag tttcttgcac agccgctact
    14581 cgctgacgtc gatgagcgta caaaaaaaca gctaaaaacc gtcctgagcg tggacagcaa
    14641 ctacttctgg ctgcgttcag atatcaccgt gaatgagatt gaactgacga tgaattcgtt
    14701 aattgtccgc atgggcccac aacacttttc tgttctctgg catcagacag gagaaagtga
    14761 gtgagttcca tgcttgagat ttttttcccg ctttgcgccg ctgatcccat ccgttggcag
    14821 cgccgtacac ccgacgtgga gcacggcatc tggcctgacg tcgccgacga atatctccag
    14881 caatggctgc aaacagacac aattcgactc tatattcccg gcgaatggat cagcgtctgg
    14941 caggttgaac tgcctgatgt gcctcgcaag cagataccga cgattctgcc cgccttgctg
    15001 gaagaagagc tgaaccagga tatcgatgaa ctgcatttcg ccccgttgaa aatcgaccag
    15061 caactggcaa ccgtagctgt gattcaccag cagcatatgc gcaacattgc gcagtggttg
    15121 caggcaaacg gcatcacccg cgctaccgtc gcgccggact ggatgtctat tccttgtggg
    15181 tttatggctg gcgatgcgca acgggttatc tgccgcatcg atgaatgccg gggatggagc
    15241 gccgggcggg cgctggctcc ggtcatgttc cgcgcacagc tcaatgagca ggatataccg
    15301 ctttcactaa ccgtggtcgg cattgcaccg gaagaactgt ctgcatgggc tggtgcggac
    15361 gccgaacgcc tgaccgttac ggctctgcca gccattatca cttatggcga accggaaggg
    15421 aacctgctaa cagggccgtg gcagcctcgt gtcagctacc gaaaacagtg ggcgcgctgg
    15481 cgggtgatga ttctgccgat attgctgatt ctggttgcgc tggtagtgga acggggcgtg
    15541 acgttatgga gcgtcagcga acaggtggcg caaagccgca cccaggcgga gaaacagttc
    15601 ttaacgctat tcccggagca gaagcggatt gtgaatttac gctctcaggt gacgatggcg
    15661 ctgaaaaaat atcgcccaca ggccgacgat acccggctgc tcgcagaatt gtcagcgatc
    15721 gccagtaccc tgaaatcagc gtcactttcc gacatcgaaa tgcgtggttt cacctttgat
    15781 caaaaacgcc agacgcttca ccttcagcta cgggctgcga actttgccag cttcgacaaa
    15841 ctgcgtagcg cactggcggc agattatgtt gtgcaacagg acgcgttaca gaaagagggt
    15901 gatgcggttt ccggcggcgt aacgttgcgg aggaaataac atgttacgcg ataaatttat
    15961 tcactatttt cagcaatggc gtgaacgcca gttaagccgt ggcgaacact ggctgacaca
    16021 acacctggcg gggcgttctc cgcgtgaaaa aggcatgtta ctggcagcgg tagtgttcct
    16081 gtttagcgcc ggatattacg ttctcatctg gcagccgttg agcgaacgga ttgagcaaca
    16141 ggagacgatg ttgcagcagc tggtggcgat gaacgcgcga ctgaagagcg ccgcgccgga
    16201 tattattgcg gcgcgaaaat ccggcacgac aacgccagca caggtatcgc gggtcatcag
    16261 cgacagtgct tcggcgcatt cagtggtcat taagcgaata gccgagcgcg gggagaatat
    16321 ccaggtctgg atagatcctg tggtatttaa tgatctgctg aactggttaa acgcgctgga
    16381 tgaaaaatat gcgctgcggg tgacacaaat tgatgtcagt gctgctgaga agcctgggat
    16441 ggtgaatgtg cagcggctgg agtttggacg ggggtaaagt atcaggagat taatatcaat
    16501 aaaattactc tcgttaacgt ggcgagagta attcccccag actttataag ctaatttttt
    16561 tcgtgaaggt tattgtcaaa tccaaaagca aaaacccgcc ttgtgggcgg gttctttaga
    16621 atagtggtgc ccggactcgg aatcgaacca aggacacggg gattttcaat ccccgtgttg
    16681 cgagtattca ggtagtgaag tgaatattct ggctggtttc cgtagtgtaa gtgattggtt
    16741 gcatacttta aagagtgtgc tatatgaaaa ttttaccggt tatatccact aaaggtggcg
    16801 aaggaaaatc aacccagtct gctaatcttg ccggttttct ggcagatgcc ggcctccgta
    16861 ctctgctgat agacggagac catgcacagc ccacggcaag caatatcttt ccgctgacgt
    16921 atgaagcgcc ggccggtctt tttgaattac tgatgcgcac ggctgacctc agtcatcctg
    16981 acaacatcat ttcccgtacg gctgtcaggg ggcttgacct catcgtctcc aacgaccccc
    17041 atgagcaact gaaaacggcc atgcttcatg cgccggatgg tcgccttcgt ctgcgtaatg
    17101 tgcttcagca tccgcttttt cagacctatg acgtcattgt ggtggattca cagggcgcac
    17161 gttctgtcat gctggaaatg attgtgctgt ccgccacgga ctctgtggtg gggatggtga
    17221 atccggttct gccggatgtg cgtgagttca tccgcggcac ggtcaatgtc atggaaaacc
    17281 ttatgcccta ccgtgaactg ggtattcccc tgccgaaagt ccgcaccctg gttaactgca
    17341 tggattatac ggcgctggcc agacagacac tggcagagct gacaggcatt atcaacgccg
    17401 gtcgctacag caaagtgctg cctgaaggtg cggtcaccct tctttcaaca cagatttacg
    17461 acctgaatat ttataaacag gggcacgcag ccggtcagcc ggtgcatcgt ctggaaaaag
    17521 tgtctgcccg ttgcagcgat tctgcgctgg tcaccataca cagtctggcc tgtgaactgt
    17581 tgccggagtg gcgtgcgttt tttgatgctc tgctggaaaa cggagggaaa agcctgtggg
    17641 cagaaaactg atatggcgtg ccgtggcttc agcacccgga cacgaggaag tgcatgtgtt
    17701 tgttgatgca tctgaccgcg cagaagcgaa aaagcatttg tatcagcgtc tggcggagga
    17761 atggcgttgt tcagtgaacc ggataacttt ccacaatatg tggaatgaaa acgaattacg
    17821 ggaaatgtca acaggtcccc gggagcctgc aggactcccg ctgcttgaat ccggaggggg
    17881 taacggcggg gttttttatg accatgagcc gttgctcctt attgcctccc ggcgtctccg
    17941 cgcatcactc gaaactgcac tcatcgtact gaagaagtgg acggtgtgcg tgggctgtgg
    18001 ttattccggg gtacgggatg gtgccggtaa gaatgctctg gtctgggtga aggtgaatcc
    18061 tgagagtggt ctggggctgt gttccggttg tgactccttt cttttgcagg aacgccagcg
    18121 tcgtcagcgg catgaccagc ttttttcatg tggtctttcc tgcaaacagc gaaagacgga
    18181 gggggcggta tgacagtctt tgtaatgcat atcccggtcg gtacagttat gagcgcaaag
    18241 atgctgtccg taccttctga ccagggtcgc caaacttacc ggtctgaaaa cgtacagaca
    18301 gttaccggga ttttattccc gacaactgtt cgtccggcac ccttttccct tttttcatat
    18361 agccaggttt ccggttctct gcggggaagt gacgcacctg gcgggctgag agatatggag
    18421 gtgaaagtat gtcttcctca gtgaaatatt ctccggttcc ggcggtctta acccgccctg
    18481 aattactggc tgaactgaac ctgctgatga cccggattgc cagtgggtgt cgtgtggggc
    18541 ttccggcgtc aggtcgtccg gtcattgcgg tgcacggtgg ttatgtttca tacagttctc
    18601 cacgcaccgg acatgttttt caggacggtt cccgcccgga gacagtgatg accgtcagta
    18661 accgggaacg gatgtgcgaa tggttcagtc aggtctggct tccgctgcat ctggctgccc
    18721 ttgaaggcga tgagaacgct tttttcagtg ctgaggtcaa catggtgcgc cgggagctgc
    18781 agagcgttcc ggggaattca gtgaactgca tgtgctgggc aagccttgct gcactggcat
    18841 ttgatgctga agagcgggtg gaacggcaga aaggcaataa tgagaacatc gcaacatatg
    18901 atgagagcat cgcaacatat gcattccgtg ctgcctgcac tgtgagtcgc tgtctgagcc
    18961 ggaatgcttc cggaggtatt ccgcgctcat accctgaatg gaaagcgatt ttgtttcccc
    19021 gtgatggtgc tgacattttc cccgacgggt ggctggaaaa tatgacccgt cgggctgaac
    19081 gtgtttttac gagtgcgaaa cgtcagtgtg gaatgctctc agccagtcag gcagcgcttt
    19141 tttctcaaca aacacgtcag tgtgccagtg agcaatgcac cggccgtatt caggccggcc
    19201 ggcatcattc agggagccac cctgacgttt cagtttatac agaacgctgt gcacaaggaa
    19261 tgtcgctttc accggtccat tcggcccgac agtgtccagt tcaacccact gattctgctg
    19321 aaacggagta aaaggatgag ccgattcaat acagttcagg ggaaaggggt tacagagccc
    19381 gtctttttcc gtcatgacct ttccgttact cagcagccag aactgcaggg tgtatttcac
    19441 ttcagattca atgccggacg gcagttgttt ttctgtattc ataaactttt tcctttaaaa
    19501 caacttgtta tgaatgaacg aatgctgtcc gtaccttcag accgggggcg gcaaactcac
    19561 cggtctgaaa acgtacagac agttaccggg attttattcc cgacagctgt acgtccggca
    19621 ccctttttcc ttttttcacg tagccaggtt tccggctgtc agagggggag ggggcgccat
    19681 ggctgaaatg tctgttctct gcgcatacga agcagagcag gccgttctgg gcggtctgat
    19741 gctggagaat gaacgctggg atgaagtggt gctgatgctg agtcctgacg atttttatgt
    19801 ggctcagcac cgggctattt tccgtgccat gtctgaactg gcggtgaatg gccagccact
    19861 ggacctgatt acactgtcag agcacctgga acatcagggc aggctggacg tactgggcgg
    19921 ctttgcttat ctggccgagc tcagcaagaa cacaccttct gcggccaaca tcatggccta
    19981 tgcaggcatt gtggtggaga aaagcgttct gcgtcagtta caggccaccg gaaacaccct
    20041 gattgctgat gtggcggcac cgaacgccac atcacgggat gtgctggatg ctgcagagcg
    20101 gcgtctgttt gcacttgcgc agaacaggac gctccgggaa cgcacggaag cctctctttc
    20161 atccgcgctt gataccgttc tgcagcaact ggaaagtgtg ctcggctccg gtggtgttac
    20221 cggtaccccc acgggatttg actgtctgga tgaaatgacc tgtggcctgc agcctggtga
    20281 cctggttctg ctggctgccc gtccgtcaat ggggaaaacc tctctggcgc tatccatgtg
    20341 tctcagcgcc gcactgcggc gaacggaaga gagcgttctg gtattcagca ttgagatgcc
    20401 gaaggagcaa ctgattctgc gcatgctttc catgctgggg cgtgtggaac tgaatcattt
    20461 gcgcagtggc aacatggatg atgaggactg gctgagggtg tcatctgcgg tcggtatggc
    20521 gcttggtgac ggaggaatgg gctctctggg tgagcgcata ataattgatg actgctgtga
    20581 gcagacgcct tcatcactgc gtgcaggcgc ccgtcgttat acacgtctgt acggtaaacc
    20641 ctccctgatt atggtggatt atctgcaact gattcgtgcg ccggagctgg aaaaccgcac
    20701 acaggaaatt gctgaaattt cccgttccct gaaagcgctg gggaaggagc tgggctgccc
    20761 ggtgctggcc ctctcccagc ttaaccggca ggtggagcag cgtgctgata aacgccccaa
    20821 taacggtgac ctgcgtgatt ccggggcgct ggagcaggat gctgacctga ttatgtttat
    20881 ttaccgcgac gaggtctata accccgactc actggcaaag ggggaggcag aaatcattgt
    20941 cagcaagcag cgtcaggggc ccacgggtac ggtacgggtc aggttcgacg gtcgttacac
    21001 actgtttacg ccctctgaga atgacgggcc gggaggatat gcgtgatggc acataaacca
    21061 ctggatctcg gggcggctat tatgcagccc ggtcgtcaga ccactggtgc cggcaatgtg
    21121 gtggcaatgg gggaaacgcc catggttctg acgctggacc agttacgtcc gaatccggat
    21181 aacccgcgca ccacgcgcaa tccgcgttat gacgatatca aaaattccat tcatgcccgc
    21241 gggctggata cggtgcccaa agtcacccgt atgccggaca gtgagccgga tgtgtatatc
    21301 ttcagtgacg ggggaaacac ccgttaccag atactgacgg aactctggaa agaaacaggg
    21361 gagccgcgtt tttaccgtat tcatgtgctg tttaaaccct ggccgggtcg gctccagtgt
    21421 gtgattggtc atctggctga aaatgaagtt cgcggtgaac tgacttttat cgagaaggcg
    21481 cagggggtcc acaaggcaag acaaatccat gaggaacaac tgggcagaac agtgtctctt
    21541 cgtgaactgg ctgatttgtt aacccatgaa gggttgccga tacataactc cacgatcagc
    21601 agaatggaag atgccctgaa gtatctgtac ccctggatcc ccgacctgat ggagtcagga
    21661 ctggggcgtc cacaggttac agcactcctc gcacttcgtc aggacgcaga acgtgtatgg
    21721 gggcagtttg ctgttgcagc cgatacggat gcggaatttg accgcgtgtt cggggaaagc
    21781 tgccggaagt ttaattcgcc ggaactgtgg tcactggaga tgttccgcga tgagcttatc
    21841 ggagatttgt tgcaggccct gccacatccc tccctggact atgaccggtg gttgctggaa
    21901 ctggatccga aagagcgaaa ccgtcgtcat cattttggtg agccggaacc ggtggtggtg
    21961 cctccttcag tccgtggcag cagtacggta caacccgggg caccggtatc cgggatccgg
    22021 tctgaagtac cggagtgtca accggatacg gatacagagg cgatgccgga aagcacagtc
    22081 tccatattcc ccggtactga gcgcgagcaa catggtatta cccggccact gaaagccgtt
    22141 tctgatactg acacagagcg cactgaaaaa tgcagtcgac cttctcagga agagagcgca
    22201 ggcagggggg acagtggggc gtcttctgct gaaaatgccc tgcgtcctga gatacagccg
    22261 gatatgtacg gtggtaagcc tgtcttcagt ggtgatgtta tggaagatga tgctgccggg
    22321 gcagtactgg catcagttat gacaggagat actgccccgg gaaccactga tgcacttccc
    22381 gcgtcggtga cgcactttgc tgtgaggcgg gatgatatcg aacatctgca gaatgaagcg
    22441 ttccgtctgg cctgggaact ggctgaatca gccggttgtg ctgaagagat tgccatggac
    22501 agggaaagcg acctgtctgc cggtttcggg gcagcagtgg agaaatgttc tcctgtagtg
    22561 gcgtttctgg cgggtctggc cggtgatgca ccgcggacag tcctccccgt gagtctgaaa
    22621 gacctgctta cagggggcag tgcaccggaa gcgtggccac tgctggatga cgaccatgcc
    22681 gtgaagctgc tgcgtcttct gagtgtgctg cgccgcctgc gagccctgca gcgcagtgta
    22741 ttacccgacg acgatgaggg ggatgagaat gatgagtgat atgtcgcatc aggaacgtct
    22801 gatcaggcgg gtcaggaaac tgctggagct gagccgtaat aacagcaatg cccatgaggc
    22861 cgggctggcg cttgcaaggg cgcaacagct gatggcgaaa cacggtatca cggagcagga
    22921 tgccggactt tcttcggtgc agacctcttc atcacagggg gcaccttctg atgctgaaaa
    22981 agttccggcg tggatgtttc gtctggtcat ggtggtgaca cagacatttg gctgccgggc
    23041 gtattacgac tggcgggcgg tgcccggagg ccggtatcgc cggtgtgtga cgttttacgg
    23101 tttcagtgaa cgtcctcagg tggcggctta tgcgtttgat gttctgtgcc gtcagctaag
    23161 ggaagcaacg gatgcgtatc ttcgtacaca gagccggaga ctgaaactga gcacccgtcg
    23221 tgcccgggcg gctcagtttc gtgatggctg ggttcagggg attcgtcagg tggtgatgcc
    23281 attcagtgtg acagaagagg aaagccggct gatgcgtctc tggatggagc agcaggatat
    23341 ggtgacggtg gactcccgtg agatgaaagc ctgtcgcggc ggtgacctgg cccgctggca
    23401 ggggtatgag gccgggaaaa atgcctcact gcatcatggt gtgggggaga gtgcccgaca
    23461 ggtggtgccg ggtctgatga tacgggaggc gtcatgaata acagcttgtc tcaggcaacc
    23521 aacggtctgc tgatgcaact ggtgatggac ctcaagagcg gctacctgcg tcgctgcgaa
    23581 tctctggggc tggcccggga ggaaatgcag atgctgcagg gactgaccat tgaggaaatt
    23641 cattacctgt caaactctga agtgtcggtt atccggctgg acattaatca tgaaaacctg
    23701 ctgcgtatgc tgcagcaggc ccggacggag cagaaacgtc tgcagcgcat tgaccgggca
    23761 ctggcgctgg gaggctccat tgaactgatg gcgttttact ttggactttc cagtgtggat
    23821 gtggcggcgc gccgtcgcat tgccggtatt gatgttcgcc cgggacgtgg catcacactg
    23881 agtgatgagg aaagtgcaga gctgtggcgt ttatggcaga agagcggcat cacggatgtg
    23941 gagagtgcgg acggactgga tgtcatgatg ctggcagctg agcaaatgaa tatcccgctg
    24001 acggcggtat ggcatgccgt gaggggatgg catcagggaa ctgcggggtt taatgatgta
    24061 tcggccagtc gccggaaact gaaccgggaa aaagcagaat gacaaggaca aatgacatgg
    24121 ccgaaattaa ctttcaggta ctgcgggaaa atgatgcggg tgaattgaca ggagtggcga
    24181 cagctggcag acctgttgcc cgccactttg acgatgggct gacactggag gtcaatcgta
    24241 cctgtactac tggtgaacgt aacgctaaca gcgcacttta tggcgctgtc tggagggcgg
    24301 caaaagatat gggctatcgc cgttgcatta catacaccag ggctgatgaa tccggagcct
    24361 ctcttcgggc agctggtttt gttcgtgtga aagaacttcc ttcaggaaaa agctgggcgg
    24421 aatcaagtgt cgccctgaag agtacacgcg atctggtcgg aagtggtggt gttccttgtg
    24481 tgctccggga aatcaggaga atgagcacta ctgtcattca catcaaaggg gaatgagata
    24541 tggctattgc agagagaacg ggtatctcct cgcgcggcga tacttacgaa atctggttgg
    24601 gagcaaactg ttatcactac cgctctgatg gttatgcagt gcttctgaaa gaaggcagga
    24661 atcgtgctta tccatgtcgg gtgacgagtg aaacgattaa atctgcgctg gcagcaattg
    24721 ttgaattgca acagaaatta actgcggcag agaagaaaaa ggagtgtgag aaccacgggg
    24781 ttcttttgcc agctgagatg ttaattcgct gtctggaaga tcaaacaggg ggacggtgga
    24841 tccgtgcaca tgaaccagta atgctgatga aacgattaat cagtaacctg aaaaaagcca
    24901 gaccagaatg taccgttcct gatgaagcgc tggcatatct ccggcgtaac tgtctggtga
    24961 atatgggcga tatacccgtt accagcagac ggatgtctga cgtataacaa aaaggaaaag
    25021 tgtaatgaca ttacccgtag acagcctgat tgcgtacacg ctggaaaaaa tgcaagagcg
    25081 gatagtgcaa aaaggccgac aacaggacgg aaaaatacaa agcggactgc tgtttaccgg
    25141 aaatgtgcat gatgccattc cgcgtcgact gcttctggat acgcgacttt ctccgctgga
    25201 taagatggcg tggatgatga ttcggctgta tgcacagaat aatgaaggtg cagttttccc
    25261 cacatatgac gaactgcaac tgcagttggc ttccccgggg aaaggaaaag cctccaggga
    25321 gactgtcagc cgggtgctgc tgatgctgcg gatcacgggc tggctgagtc tgtgcaaacg
    25381 ggtgcgtgat gagaaagggc gggtacgggg caatatttat gcccagcacg atgaaccggt
    25441 gacgtgccgg gatgctgaaa tactggatcc acgttatctg gatatggtgg cagatgcctg
    25501 tatgagccgg aaccggacca taagtcagac ggcccgggat attctgtgcg aaataaaaaa
    25561 tgatccggga atgcgtcatt atcacagcca tatcagtcaa ctggaagccc ggatagggag
    25621 tgcccagaca ccgggacaga tggcggcccg tcagctggtg actgaggaca gaatgcagcc
    25681 gggttcggaa accgaaccgg gggtagaaac acagagttcg gtattgaaaa tgctgagttc
    25741 ggattccgaa ctcagtgaaa aacaggggca aaaaatactg agttcggaaa ccgaaccatc
    25801 agaaaaatca gaaggttatc acagagttcg gaaaccgaac cattacgtac gtaatatcac
    25861 acacagtgtg attaaaaaca cgtacgtatt gcctgaagcg ctgacagggc aaatcaggga
    25921 ggaggaggcc attatgctga ccagtcagtt gcaggcgctg ccggaagaac tggcgcaggg
    25981 ggttctggaa agtctgtcag ggatgctggg cagccactcg ctgcagaatc ctgtgggctg
    26041 gttgcttgca gtgctgaagc gagcccggga gggaaatttt tatccgccga agcagaaaca
    26101 acgtgttgtt gtgccagcct ccgtactggc aggagaaagg gggcgagcta ctgcgcaatc
    26161 agtttcatcg gggaaacgta ctgaaccgat ttcagcagaa cgtctgagtg gcattgtggc
    26221 tggactacgg gcctcgctgc gcagatgttc tgcgatgaga tctgagtgat tgcaatacgg
    26281 gctatgttcg cgggaacaaa aatcaaccag tgccgccagc gactggttaa aaagaaaaca
    26341 gaatcgggaa ctggtcaggt gaaacaaggt ggtaaatagc atgcagaaat acaaacagga
    26401 aagtctgagt gcagccatga aagaaagtgg cctgttgatc gctaaaattt gtgcctgcgt
    26461 gattgccgga gtggtggtta tctgggggct ggtgacaggc acagagaccc tgctcactgc
    26521 gctgaaaaca atgcacgaac agtacgataa cagtatgtgt tatatcgtat gtgccacagg
    26581 gtggtttttc ctggtgttaa gtaactggct gtgggtcaca tacagtcttt ataaagcgcg
    26641 tacaggcagt acagtcggag ggggcctgca atttatgttc ctcctgagtc tgctggcaga
    26701 atgcttttca ttttttgtga ttttctggtc attgtctcat ctggatataa ccgacattca
    26761 gttgctcgtg atggggataa cgacattaat gctgtttttg tctctgaacg ttaaggtagt
    26821 agtcatcatt aaccatgttg ccagtggcaa cagtgattaa aaaatgagcg gtgttgccag
    26881 tggcaacaga ggttaaaaaa tgagcagtgc tgccagtggt aacggtgatt aaaaaatgag
    26941 cggtgttgcc agtggcagca gagattaaaa aatgagcagt gctgccagtg gcagcagaga
    27001 ttaaaaaatg agcagcgctg ccagtggcag aagaggttaa aaagcgaaca gtgctgccag
    27061 tggcagaaga ggt
//
LOCUS       DAZKHK010000072        26706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKHK010000072 DAZKHK010000000
VERSION     DAZKHK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.072"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            128..1825
                     /gene="ypdA"
                     /locus_tag="ACHOS5_002953"
     CDS             128..1825
                     /gene="ypdA"
                     /locus_tag="ACHOS5_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP9777742.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1840..2574
                     /gene="ypdB"
                     /locus_tag="ACHOS5_002954"
     CDS             1840..2574
                     /gene="ypdB"
                     /locus_tag="ACHOS5_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP9777743.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            2587..3444
                     /gene="ypdC"
                     /locus_tag="ACHOS5_002955"
     CDS             2587..3444
                     /gene="ypdC"
                     /locus_tag="ACHOS5_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9777744.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPTSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(3447..5942)
                     /gene="ptsP"
                     /locus_tag="ACHOS5_002956"
     CDS             complement(3447..5942)
                     /gene="ptsP"
                     /locus_tag="ACHOS5_002956"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9777745.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFIDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(5967..7004)
                     /gene="ypdE"
                     /locus_tag="ACHOS5_002957"
     CDS             complement(5967..7004)
                     /gene="ypdE"
                     /locus_tag="ACHOS5_002957"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9777746.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDSDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRFT
                     RETVVQLTDFR"
     gene            complement(7004..8089)
                     /gene="ypdF"
                     /locus_tag="ACHOS5_002958"
     CDS             complement(7004..8089)
                     /gene="ypdF"
                     /locus_tag="ACHOS5_002958"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9777747.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQFELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEYIRRFIQAGMSE
                     CEIAAELEWFMRRQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQAGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(8104..9351)
                     /gene="fryC"
                     /locus_tag="ACHOS5_002959"
     CDS             complement(8104..9351)
                     /gene="fryC"
                     /locus_tag="ACHOS5_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9777748.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSIMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(9373..9699)
                     /gene="fryB"
                     /locus_tag="ACHOS5_002960"
     CDS             complement(9373..9699)
                     /gene="fryB"
                     /locus_tag="ACHOS5_002960"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9777749.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9918..10883)
                     /gene="glk"
                     /locus_tag="ACHOS5_002961"
     CDS             complement(9918..10883)
                     /gene="glk"
                     /locus_tag="ACHOS5_002961"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP9777750.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEENKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11087..12343
                     /gene="yfeO"
                     /locus_tag="ACHOS5_002962"
     CDS             11087..12343
                     /gene="yfeO"
                     /locus_tag="ACHOS5_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP9777751.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIITVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            12458..12784
                     /gene="ypeC"
                     /locus_tag="ACHOS5_002963"
     CDS             12458..12784
                     /gene="ypeC"
                     /locus_tag="ACHOS5_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP9777752.1"
                     /translation="MFRSLFLAAALMAFTPLAANASEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDSGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12925..14163)
                     /gene="mntH"
                     /locus_tag="ACHOS5_002964"
     CDS             complement(12925..14163)
                     /gene="mntH"
                     /locus_tag="ACHOS5_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9777753.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            14499..15701
                     /gene="nupC"
                     /locus_tag="ACHOS5_002965"
     CDS             14499..15701
                     /gene="nupC"
                     /locus_tag="ACHOS5_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP9777754.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15751..17952)
                     /gene="pdeA"
                     /locus_tag="ACHOS5_002966"
     CDS             complement(15751..17952)
                     /gene="pdeA"
                     /locus_tag="ACHOS5_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9777755.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQ
                     ENEKLYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDG
                     NQETLQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDL
                     LLYAQPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKW
                     LATHPCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNA
                     ESSMYNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAM
                     IVRSITDLAKAKSLSVVAEFVETPQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(18159..18234)
                     /locus_tag="ACHOS5_002967"
     tRNA            complement(18159..18234)
                     /locus_tag="ACHOS5_002967"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18199..18201),aa:Ala,seq:ggc)
     gene            complement(18274..18349)
                     /locus_tag="ACHOS5_002968"
     tRNA            complement(18274..18349)
                     /locus_tag="ACHOS5_002968"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18314..18316),aa:Ala,seq:ggc)
     gene            18570..18929
                     /gene="yfeC"
                     /locus_tag="ACHOS5_002969"
     CDS             18570..18929
                     /gene="yfeC"
                     /locus_tag="ACHOS5_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9777756.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18931..19323
                     /gene="yfeD"
                     /locus_tag="ACHOS5_002970"
     CDS             18931..19323
                     /gene="yfeD"
                     /locus_tag="ACHOS5_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9777757.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(19363..20571)
                     /locus_tag="ACHOS5_002971"
     CDS             complement(19363..20571)
                     /locus_tag="ACHOS5_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9777758.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLRDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGIVKNVTVCQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILNQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQICGYTANADVNGARNILAAGHAVLACGEMVQSCRPLKQ
                     EPTEMIQATA"
     gene            20579..21006
                     /gene="tnpA"
                     /locus_tag="ACHOS5_002972"
                     /pseudo
     CDS             20579..21006
                     /gene="tnpA"
                     /locus_tag="ACHOS5_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(21121..22536)
                     /gene="gltX"
                     /locus_tag="ACHOS5_002973"
     CDS             complement(21121..22536)
                     /gene="gltX"
                     /locus_tag="ACHOS5_002973"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9777759.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLNLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            22795..22870
                     /locus_tag="ACHOS5_002974"
     tRNA            22795..22870
                     /locus_tag="ACHOS5_002974"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22828..22830,aa:Val,seq:tac)
     gene            22915..22990
                     /locus_tag="ACHOS5_002975"
     tRNA            22915..22990
                     /locus_tag="ACHOS5_002975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22948..22950,aa:Val,seq:tac)
     gene            23037..23112
                     /locus_tag="ACHOS5_002976"
     tRNA            23037..23112
                     /locus_tag="ACHOS5_002976"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23070..23072,aa:Val,seq:tac)
     gene            23117..23192
                     /locus_tag="ACHOS5_002977"
     tRNA            23117..23192
                     /locus_tag="ACHOS5_002977"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23150..23152,aa:Lys,seq:ttt)
     gene            complement(23303..24187)
                     /gene="xapR"
                     /locus_tag="ACHOS5_002978"
     CDS             complement(23303..24187)
                     /gene="xapR"
                     /locus_tag="ACHOS5_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP9777760.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(24228..24386)
                     /locus_tag="ACHOS5_002979"
     CDS             complement(24228..24386)
                     /locus_tag="ACHOS5_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFP9777761.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(24439..25695)
                     /gene="xapB"
                     /locus_tag="ACHOS5_002980"
     CDS             complement(24439..25695)
                     /gene="xapB"
                     /locus_tag="ACHOS5_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP9777762.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(25756..26589)
                     /gene="xapA"
                     /locus_tag="ACHOS5_002981"
     CDS             complement(25756..26589)
                     /gene="xapA"
                     /locus_tag="ACHOS5_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP9777763.1"
                     /translation="MYQAHFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVQGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
BASE COUNT         6729 a         7119 c         6486 g         6372 t
ORIGIN      
        1 cattgctaat acaccattta tctttcaatc cccttttaaa attgtggttt ttcctcaaaa
       61 acggcctgct ggtcatcagc cttcaggtta cctatcatag aggtttaatc cttattcaga
      121 gtcacccgtg cacgaaatat tcaacatgct gctggcggtc ttcgatcggg ccgcgttaat
      181 gcttatctgc ctgttttttc tcatccgtat ccgcctgttt cgcgaactgt tgcacaagtc
      241 ggcacactca ccgaaagagc tgctcgccgt taccgccatt ttctcgctgt tcgccctgtt
      301 cagcacctgg tccggcgttc ccgtagaagg ctcgctggtg aacgtacgta ttatcgcggt
      361 gatgtcaggc ggaattctgt ttggcccgtg ggtgggcatc attaccggcg tgattgcggg
      421 tattcaccgt tatttaattg acatcggcgg cgtaacggcg atcccctgct ttatcaccag
      481 cattctggcg ggttgtatat cgggctggat caacctgaaa atccccaaag cacagcgctg
      541 gcgcgtcggt attctcggcg gcatgttgtg tgaaacgttg accatgattc tggtgattgt
      601 ctgggcacca actaccgcat tggggatcga tatcgtctct aaaatcggga ttccaatgat
      661 cctcggcagc gtctgtatcg gctttattgt gttgctggta caaagtgttg agggagaaaa
      721 agaggccagc gccgcgcggc aggccaagct ggcgctggat atcgccaaca aaacgctgcc
      781 gctgtttcgc catgtcaata gcgagtcatt acgcaaagtc tgcgaaatta tccgcgatga
      841 cattcacgcc gatgcggtgg cgatgactaa taccgatcat gtgctggcct acgttggcgt
      901 gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc gccaggcgat
      961 gaattatgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac cagagattca
     1021 ttccatgctg gtgatcccat tgtgggagaa aggcgttgtg accggaacgc tgaaaattta
     1081 ctactgccac gcgcatcaga taacctcgtc attacaggaa atggcggtcg gtctgtcaca
     1141 aatcatctcc acgcaactgg aggtgtcacg cgccgagcag ctgcgtgaaa tggcaaataa
     1201 ggcagagctt cgcgccctgc aaagcaaaat taatccccat ttcctgttta acgctttgaa
     1261 cgctatttca tcgtcaatcc gcctgaatcc ggatactgct cgccagctga tctttaatct
     1321 gtcgcgttat ctgcgctata acattgaatt aaaagatgat gagcaaatcg atatcaaaaa
     1381 agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg gcgacaagct
     1441 gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc tgctgatcca
     1501 gccgttggtg gaaaacgcca ttgtccacgg tatccagcct tgcaaaggta aaggcgttgt
     1561 caccatcagc gttgcagagt gcggtaatcg ggtacgtatt gcggtgcgag ataccgggca
     1621 cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaac gaaatgccgg gcaataaaat
     1681 tggcctgctg aatgtccatc atcgcgtgaa gttattgtat ggcgaggggc tgcatatccg
     1741 ccgcctggaa ccaggaacgg aaattgcgtt ttacattcct aaccaacgca ccccagtcgc
     1801 ctcacaggct acgttattgc tttgagccgg agtgatattg tgaaagtcat cattgttgaa
     1861 gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag ccagatggag
     1921 attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca taaccgcgtc
     1981 gacgccattt ttctggatat caatattccg tcgctggatg gtgtgttgct ggcacaaaac
     2041 atcagccagt tcgcccataa accgtttatt gtgttcatca ccgcgtggaa agaacatgcg
     2101 gtagaagcgt ttgaactgga agcgtttgac tacattctca aaccgtatca ggagtcacgc
     2161 attaccggga tgctgcaaaa actggaagcg gcctggcaac aacagcagac cagcagtacg
     2221 cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga tgagcgaatc
     2281 atcgtcacgc ccattaacga tatctattac gccgaagcgc atgagaaaat gacctttgtc
     2341 tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgagttttg cagcaagctg
     2401 ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa caaaatccgc
     2461 gagatcgaac cgtggtttaa taacacctat attctgcggc tgaaagatct ggattttgaa
     2521 gtgccggtca gccgcagcaa agttaaagaa ttccgccagt taatgcatct ttaatcggga
     2581 actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga tctgttcagc
     2641 ggcctggtgc ttaacccgca actactgggg cgcgtctggt ttgccagcca gcctacctca
     2701 ttgccagtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct acgcggcgaa
     2761 tacggcaatc tgctggaagc aaagcagcaa cgtatggtgg aaggagaaat gctgtttatt
     2821 ccggcacgcg cggctaattt accggtcaac aacaaaccgg tgatgctgtt aagcctggtg
     2881 ttcgcaccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc gttgttacat
     2941 cctgctcgcc agatccagct tcccagcctg caacgcggtg aaggtgaagc gatgcttacc
     3001 gccctcaccc atcttagccg ttcgccactg gagcagaata tcattcagcc gctggtgtta
     3061 agtttgctgc atctttgccg taacgtggtg aatatgccgc cgggcaattc gcaaccgcgc
     3121 ggcgattttc tctatcacag catttgtaac tggattcagg ataattatgc ccagccgctc
     3181 acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc aaaactgttt
     3241 gctcagcatg gaacaatgcg ttttatcgag tatgttcgct gggtacgaat ggcaaaagcg
     3301 aggatgattt tgcaaaaata tcatctgtcg attcatgaag tggcgcagcg ttgcgggttt
     3361 ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttggtctgac gccgggagag
     3421 tatagcgcac gttttcaggg ctaacgtcag aaggttaatt ccgtttccag cagcgtcaga
     3481 atactttgtg catcttgcgc ggcaaacagc gactggcgga agtttttatt caccagtttg
     3541 cgcgccagct gcgagaagac tttcacatgg ttcatccctt cgttagcacc cagcgtcagc
     3601 atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt caccaaccgg
     3661 gcaatgctga tactggaatg acggatccac tgtgatttgg tgtgcgggat cgccacgcca
     3721 aaaccaacgc cagtagtaac aatctcttcc cgctgccaga catcttcttc cagctcgaac
     3781 ggatgttcag tacgcccgtt aacaccgagg ttaccgcaca ggaactggat cgcctgctct
     3841 ttattactaa aggactgatc aataaagata ttctctagtg ccagcagtgg gcgaacgtct
     3901 tcttccggcg caaacgcggt aagtaacgct tcaatttcct gcgcgctgcg gcactcgcac
     3961 gcctgacgcg ccagttcccg acacgcctcg ctatccagtt gacgaagctg gcttttcact
     4021 gccggaatac gcgggctact catgcttagc tcatccaggc ccagccctag cagtagcggc
     4081 agataacgac tttcaccgcc cagttcaccg caaataccta cccatttgcc gcgctgatgc
     4141 gcagcggtaa caatctgctg caacatgcgc aggaacgatg gcgtaatcgg gttatacagc
     4201 ggcgatacgc gcgggttatt acgatcgact gcatacagat actgggtcat atcgttagaa
     4261 ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca caccgacggc
     4321 acttccacca tgatacccag cgtaatcgtc tctgcatgac gcaggccatc gcgcttaagc
     4381 tcaacgatcg ctttttggag ctcacccttc acccataaga tctgatcgag actgtggacc
     4441 attgggatca tcaactgggc gttgccgaaa ctggcagcgc gcaaaatcgc ccgcaattga
     4501 gtacggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc gaggaacggg
     4561 ttctcttcct ggggaatgtt gaggtaagga atgcttttat cgccaccgat gtccatcgtg
     4621 cggaagataa tcggcttgtc gcccgccgcc agtagcacct gctggtacgc ttcaaactgt
     4681 tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg aaacagccca
     4741 acgccttccg ctccgttggc aaacgcgcct ggggcttcca gagctgtgcc aatattcgcc
     4801 gcaatatcaa tacgcttttt atcacgggaa taggccagct gcgcggcagc ttgcgcctgt
     4861 tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct caccgcgtca
     4921 ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg cccggcataa
     4981 cgggcaatcg catccagcgg caggccgctc agtaccggga tcgccgaagc gcgggccaga
     5041 atcagggtat gcgaggtgcg cccggttttc tccagaatca tgcccgcaag attttttaaa
     5101 tcgaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt cggtttttca
     5161 agcaccagat tattgcgcgg ttttagttcc ggccaggtga tatgcagcaa ctgttcgctg
     5221 atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc agaaagtttg
     5281 gcacaaacct gctccatatt gctgatgatc gccgccccca gcccctgatg ctgttctgcc
     5341 atcaggtgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa atgggcgctg
     5401 aggatagttt tgctttcgcc gtcacgctca cgcaattgct ggttcagttg ctcggcaagc
     5461 gttgccagac tgtgctccag tagggtggaa tcttgcgcac tcgcagggat cacccgataa
     5521 ctgtcgaggc tgtcgctctg taacagggtc agtgtaccca cgccaacgcc gcttgccagc
     5581 acgttgccgt acagtaaatc cgggttcagg cggcttaatg aacgcggcag cggatgcgcc
     5641 gtcagttctg ccagcgtcgg ctggacgcta tcgctgtcga taaagcgcac ctggatatac
     5701 tcttccagca ctcgccgcgc ctgctcttca tcgctaccgc taatgttcag gctgcaactg
     5761 tcattaaata aagtgccagt gccaatcagc gccagcgagc ttttcgcatc ggcctttgcg
     5821 ttctggcgat ggttgatgaa ggtgatttcg ctttgccact ggctgcactg ctctttaagt
     5881 tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg aatcgttaac
     5941 ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac aaccgtctca
     6001 cgcgtaaaac gttgaataag ggcagataaa agttgctcca tctgcaaaat gtcacggcag
     6061 tcggcaatcg atgccgcgca atgtccgtgg cgggttgctg gccccatcac cactgtgggt
     6121 acgccagtgc cggttaaatg caccgccccg ccgtccgttc cgccgttgct gaacatatct
     6181 gactgcaacg gtacgccaat ttctgccgcc acggtttcga tccacgcggt aagttttggc
     6241 ggcgcaatca gtgacttgtc gcttaacacc agcatcggcc cgttaccaat ctggcgatgg
     6301 ttagctgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaagcac aatggcgaca
     6361 tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta atcccacctc ttcgctggaa
     6421 cttgccacca gccacacttc cgcaggcaat tcggcgtcgt gcagctcacg cagtaacgtc
     6481 accagcagat agcaaccgag gcggtcatca aaggctttcc ccatcactcg ctggtgaggg
     6541 agaacctgaa aagtggtatc gaaggtgacg cgatcgcctg gacgaattcc tgcctgcatc
     6601 acttcgtcac aggagcgcgc gccaatgtcc acgcgcatgg cgctgacgtc attcccctgc
     6661 cggtcgctgt caagcaggcc tggaattttg cactcttcac gggtagtgat acgcaccgac
     6721 tgaagctggc gggcagccat gcgtacattg ccaaccggca acacatcgat cgccccttcg
     6781 cgggagatgc tgcgcaccat aaatcccact tcgtccatat gcgcacagat catcaccttc
     6841 ggacctgtcg actcattgag tcggatcagc accgatccca ggccgtcaaa acggacctct
     6901 ttttgcaggc gatccgcttc ttccagcagg atctgccgta cttcctgttc cgaggaggcg
     6961 atcgcatctg cctcgctcaa cgcttttaat aacgataaat ccattatgcc tctcccgtga
     7021 gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcggggtg accagcacaa
     7081 catcttcgat gcgcacgccc ccttgccctg gcaaataaat ccctggctcc acggtcagta
     7141 acatacctgc ctgtagcgtc gtggtgtccc gcggtgaaaa acgcggttct tcatggactt
     7201 caataccgat agcatgaccg gtgttatggc cgaaaaactt gccataaccg gcctcggtaa
     7261 tcacgcggcg cgcggcatcg tcaacctgct ggcagcgcac gccggggcga attacagaga
     7321 ttgccgcgag ctgtgcctgc aggacaatct gatagacgtt aaacagcggg tgagattcgg
     7381 cgctcacccc ttcgccattc accagcaagg tgcgcgtcat atcagagcag tagccctgat
     7441 acagcgcacc gaaatcgaga gtgacaaact cgcccgctgc aacaatcttg tcgctggctt
     7501 taccgtgcgg cagcgcccca cgccagccac tcgcgacaat ggtatcgaaa gaggcttttt
     7561 ctgcgccctg ccggcgcata aaccactcca gttcagcggc tatctcgcat tcgctcatcc
     7621 ccgcctgaat aaagcggcga atatactctg cgccacgatc ggcaatcccg caggcaaggc
     7681 ggattttctc cacctcttct ggcgttttga tttgccgcag cacatccggc gtggcgctaa
     7741 ccagtttcgc attgagttcg aactgccagc ggtgcgcggt ttcccaactc acctgctggc
     7801 cttcaaaacc gagcgtttgc aactgctcat cggcaatgat ttgtctgacg atggtggcaa
     7861 gcgtgtgcat cgcgtcgagc aaatgcagct ggtaaccttg cgtgcgggct tctacatccg
     7921 cgtaatagcg cgaatccacc agaatgtgcg cactttcacg gctaatcacc acataacctg
     7981 atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga agcaccgcat
     8041 ccagttgttg cgccttaagc cagtcgcgca gcgaagcgag taatgtcatc aggattgtcc
     8101 ttcttacagg ctatcgatta acaatttgcc tttacggaac atcatcagac gcaggaacac
     8161 caccatcagc gcagttatga ctgcccccag cgcgatcccc gccatgtaga cgccgaggtt
     8221 agtgaccagc ggccatgccc agatagcgga ctccgggaac cattgcactg cgcccagcca
     8281 gactgcggcg gttgagccga caatcgcgcc aaccatatac gacggaatgg cggtgatagg
     8341 gctttccagt gcaaacggaa tcgcaccctc actgatcccc ataaaggcaa ggaacatcgc
     8401 ggtcttaccc tgcggataaa gctgggcgtt gaacaagcgt ttgccagtta agcgacgatc
     8461 gataatggtc gccagaccca gaccaatcgg cggaataacg atagcgatag aacgtgcagt
     8521 gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgccg ctttgttgat
     8581 cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc attgcgtaca tcagcgcgcc
     8641 cttctcacct gcagcggtca gcacggtacg gataccgccg ttgatccagc caccaaaggg
     8701 ggtgatgacg tagtacatcg ccagcatgac aaaaatggcg gaaagaatcg gtagcaaaaa
     8761 tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca tccatttcac
     8821 aaggtaaccg gcaacgatag aaataatcag cgcaccaatg aaggtggacg gcaccggcga
     8881 agaggtcgcc cattgcataa tgctgggatc gaagttcagc agttgcgtcg gctgggtaga
     8941 catcaagccg ccgataaaac cagccgggaa cgccagtttg ccaccgatag agttcgccac
     9001 aaaagcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg attgcgacag
     9061 ccaggcaaat ttaagtaagg agaggtcgaa accagagaat ttcccgctat taagagcatc
     9121 catgatgccg atatcagcag gaattttaag ccagctataa gcaatcagct gactgaaagc
     9181 gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg acatcacatg
     9241 ctgcgggagt tcaccccaga agctggattt agaggcctgc ggattcttaa ctgccgctgc
     9301 cgcaccggat gcgccatgca caacggttgc actgcgtttt ttaatggcca taacaattat
     9361 ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt tgatgattcc
     9421 cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg attcgaaacg
     9481 ttcgttatct tccggggtaa ctgccacgga gtggatgatg atggtcgctt cggcgatatc
     9541 ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg gtttcaattt tcacttcata
     9601 accggcttct accgccgctt cttccagcgc ctgagcggcc ataaaagtgt gagccaggcc
     9661 cattgggcag gcacataagg caatcagttt cttactcatc gttaattcct cgttcagtga
     9721 tagttgacga gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga caaataatgc
     9781 atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc ttgtgagggg
     9841 gacacaaaga aaatttaatt aactgtatga tttaaaagat tatcgggaga gttacctccc
     9901 gatataacag gaaggattta gagaatgtga cctaaggtct ggcgtaaatg tgcaccggaa
     9961 ccgagaaggc ccggattgtc atggacgatg agatacaccg gaatatcatg gacatattct
    10021 ttaaagcgcc ctttatcttc aaatgcggcg cggaaaccag aggctttgaa gaactcaagg
    10081 aagcgcggca cgataccacc cgcaataaac acgccgccaa atgtcccgag attgagcgcc
    10141 agattgccgc caaaacggcc cataatgacg caaaacagcg acaacgcgcg gcggcaatcg
    10201 gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt ttctggcagg
    10261 cggttgtcgg ctttaacaat tgcgcgatac aaattcacca gtccagggcc agaaagcacg
    10321 cgttccgccg aaacatgacc aatttccgca cgcaatattt cgaggataat gccctcttct
    10381 tcactattcg gcgcaaaatc aacgtgaccg ccttctcctg gcaagcttac ccaacgctta
    10441 tcgacatgga ccagatgcgc aaccccaagc cctgttccgg caccgtaaac cgcaataggc
    10501 ttaccttcta ccggttctgc gccaccaaac tgaatcagat gctctttttt cagcatcggg
    10561 atcgccatcg atacagcagt aaaatcgtta ataatttcca gatggctaaa accgagattc
    10621 tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac ccagtcaccg
    10681 gtaattgggc aagcgatggc aatacagccg tctttcacct cgaccttatt ttcttcaaga
    10741 taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt cttagcctgc
    10801 gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc gcccacatca
    10861 ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa taattctttc
    10921 tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct ccctgtaaat
    10981 atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg atgccttttt
    11041 gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc tccatccgcg
    11101 agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg cgtccagtct
    11161 tattctgatt atggtgatga aaatcgcctc ggtattacag aatttgctct ggcaacgact
    11221 gccgggaact ctggggatag cccaggattc acctctctgg atcatcggtg tattaacgct
    11281 aacgggtatt gcggtggggt tggttatccg tttcagccag ggtcatgccg gaccagatcc
    11341 cgcctgtgaa ccgcttatcg gcgcaccggt tccgccctct gcgctaccag gacttatcgt
    11401 agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc cgatcataac
    11461 cgtcaatatc gcccttgccg tcgccattgg cgctcgtctg ttaccgcgcg tcaaccgaat
    11521 ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggca cacctgttgc
    11581 ggcggcattg atattttcgc aaaccttaaa tggcagtaat gaagttccgc tatgggatcg
    11641 tctctttgcg ccgttaatgg cggcagcagc tggtgcactt actaccggat tatttttcca
    11701 tcctcatttt tcactgccca ttgcgcatta cggacagatg gaaatgaccg atattctcag
    11761 cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat ggtgcttacc
    11821 acggttgcac gcgatgatgc atcaaatgaa aaatccggtg ttcgtgctgg gtattggcgg
    11881 atttattctc ggtattctgg gggttattgg tggaccggtt tcgctgttta aagggctgga
    11941 tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact ttttgctggc
    12001 ggtgataaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg gtgggcgaat
    12061 cttcccggca gtgtttgtcg gcgtggcatt agggttgatg cttcatgagc acgttcccgc
    12121 cgttccagcg gcaataaccg tttcctgcgc tatactcggc atcgtgctgg tggtaacacg
    12181 cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca cattgctacc
    12241 gctgctctgt atcgtcatgc tcccggcatg gctgttatta gcaggtaagc cgatgatgat
    12301 ggtcaatcgt ccgaagcaac agccacccca cgataacgtt tagcaaaaaa tgctctcctt
    12361 tatgatttaa gagttatggc gatgatacgg cgctttacct ccccgtaata ttgcgttaac
    12421 aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac tgtttctggc
    12481 ggccgccctg atggcattta ccccgcttgc ggcaaacgca agtgaaatca ccctactgcc
    12541 atcaatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg acggtggtca
    12601 ctggcgcgac cgtgactact ggcaccgcaa ttatgaatgg cgtaaaaacc gctggtggcg
    12661 tcatgatagt ggctaccatc gtggctggga taagcgtaaa gcgtatgagc gtggctatcg
    12721 tgaaggctgg cgcgatcgtg acgatcatcg tggaaaaggc cgcggacatg gacaccgcca
    12781 ttaaacgcat ccgtcaatgg agcacaatgc ctgatgcgac gctagcgcgt cttatcaggc
    12841 ctacaaacca gctctttaac gcgtagggcg gataaagcat ttacgccgca tccgccaatg
    12901 gtgccaaatg cgacgctcat tcaactacaa tcccagtgcc gttcctacca gtaaccagat
    12961 attcagcgcc actaccagca ccacaatcac ccagcctgtc tgttttacgc gtttactgtt
    13021 caccagatcg cccatcaact tgctgtcact ggtgaaaatc aacagtggaa ccagcgccag
    13081 ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg gatctaatcc
    13141 catcagaatg acgataaatg acggcagcat ggtgactgta cgacgcaccc acagcgggat
    13201 atgaaagcga atgaagccct gcatcactac ctgccccgcc agcgtcccca ccaccgttga
    13261 agacagtccg gcagcaacca ggcttaatcc aaagaccgtt gccgcagcat ggcttaacag
    13321 cggttgcagc gtcagataag cctcatcaag atcggcaaca ccagtatgac cagaaaagtg
    13381 gaacgccgcc gcagctgtag ccatcatcgc cagattgaca aaaccggcaa tcgtcatggc
    13441 gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc catgtaaatg
    13501 ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc ctaacacgcc
    13561 cgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc ctttacccag
    13621 ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg caacaaacaa
    13681 cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca gcattaaaat
    13741 aaggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac caagaatgag
    13801 tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg caataatttc
    13861 tgcctgaacc caatagaacc acacgacggg acgcgggtag tgatcgcgaa tctgctccgc
    13921 cagattttta ccggtggcaa tccccagttt ggccgagagg atttgaatca gcatcgccat
    13981 caggttagcc caaacgacaa cccacaataa ctgatagccg aagctggcac ccgcctgaat
    14041 attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg caggtcccat
    14101 taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc gatagttcgt
    14161 catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctacgttaa atgagaatga
    14221 ttatcaaatt catttaaatg ggttgtgatg atttctctga tagaccagga ttatgactac
    14281 gaaaagatag atgactcgaa tgttatggtt atgtttaata ttagcacatt tacataacct
    14341 tccgcttcca tacacaacat agcagaaatg tatgacagat cactattttt gaagcttgtc
    14401 acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt acatagaatg
    14461 tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc cttcattttg
    14521 tactggcact tgccgttgtt gcgattctcg cactgcttgt aagcagcgac cgcaaaaaaa
    14581 ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg tggttcttcc
    14641 tgaactccga cgttggtctg ggcttcgtga aaggcttctc cgaaatgttc gaaaaactgc
    14701 tcggttttgc caacgaaggg actaacttcg tctttggtag catgaatgat caaggcctgg
    14761 cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcgctg atcggtattc
    14821 tccagcatat tcgcgtattg ccggtgatta tccgcgcaat tggtttcctg ctctccaaag
    14881 tcaacggcat gggcaaactg gaatccttta acgccgtcag ctccctgatt ctgggtcagt
    14941 ctgaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgcaat cgtatgtaca
    15001 ccatggcagc aacggcgatg tccaccgtgt cgatgtccat cgttggtgca tacatgacca
    15061 tgctggagcc gaaatacgtc gttgcggcac tggtactgaa catgttcagc acctttatcg
    15121 tgctgtcgct gatcaaccct taccgtgttg atgccagtga agaaaacatt cagatgtcca
    15181 acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg gcaggtttca
    15241 aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc gccgcgctga
    15301 acgcactgtt tgccaccgtg actggctggt ttggctacag catctccttc cagggcatcc
    15361 tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt gaagcactgc
    15421 aagtgggcag tatcatggcg accaaactgg tttccaacga gttcgttgcg atgatggatc
    15481 tgcagaaaat tgcttccacg ctctctccgc gtgcggaagg catcatctct gtgttcctgg
    15541 tttccttcgc taacttctct tcaatcggga ttatcgcggg tgcggttaaa ggcctgaatg
    15601 aagagcaagg taacgtggtt tctcgcttcg gtctgaaact ggtttacggc tctaccctgg
    15661 tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta agaccataca taaaaaagcc
    15721 ggggataatt ccccggcttt tttacgcctg ttaatcagct aatggctgcg gacgaccaat
    15781 taaatatcct tgcagatatt gcaccccaag tttatgcaat agcgcctgct gctgtggcgt
    15841 ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg taattgatct
    15901 cacaatcatc gcatccagcg tgttcgtgac gatatctttc acaaagacgc catcaatttt
    15961 gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatatccgg tgccaaagtc
    16021 atcaattgca atccggaaac caaacttatg cagctgctcg atgttgtaca tgctgctttc
    16081 tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg gggagatgta
    16141 ataacgctta aacagacgaa taatccgccc ggcaatattc ttttgcagca gcgtgagcgg
    16201 cattaaattg actgaaaagc gtggaccttt tttgtcgcaa gggtgtgttg ccagccactt
    16261 caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact gagcaataag
    16321 gggcagaaac ttatccgggg tcataatgcc gccgtcatac ttcagtcgcg cgaggatctc
    16381 atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca aatcaccctg
    16441 atctaacgcc gtgcgaatgg tattcagcaa tagcacctgt ttagtggttt gcccggaaac
    16501 catctcctct ctgctatcca gcgccagcac atgatgatgt gcgcaggatt gctccgccag
    16561 ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa aacgccccca
    16621 ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc gactattcag
    16681 gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca ctaacagcag
    16741 ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca gcagcgtacg
    16801 gtaaattgag cggatacagt gaacgcgcat cattaagccg taatgacgac tcataaactc
    16861 aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg gcgcttgctc
    16921 cggcgcttgc tccaacgcac gaaagttggg tagaagcgtt aacggatcgg tcagcgcctg
    16981 caaatgccag cggcggttaa gccattcact acgatgataa atacgcacca tatagagcag
    17041 gcaaacacta aaggaaatca gtactgcgag aataaatgcc agcgaatatt cggttagtac
    17101 cccttgcaaa aagttctggt tgtaattcag aaggcaaagc gttgaaacag cccaggtgag
    17161 atttaaaaac ggatagcgaa gcttaccgac accgagggta aagatgataa agaaaacagg
    17221 taccaggtaa ccggcaataa agtcattttc atacggtgtg cacatcagaa gtagcagcac
    17281 gctaagagct gccagccagc ttaaggtaaa tgcgcgtttc tctttgctca acgacggagc
    17341 gatatcccta cgccacaata tctgtgcaaa gtggggactt acaatcatgc gggtgagata
    17401 gtaaaaaagc atgttgtaaa tcagcactgc ggtgaaaagg cttagcaaat caacgaccgt
    17461 gaaaatggca tccgcatcgc cgaaaaaggt agatatcttt agcggaaaat caaagaaatt
    17521 tcccacaaga tacatgctgc atttgatgcc aatcggcgcc accaaaccaa gccagaacag
    17581 acgttgccag acgtatcggc tggtcaatcc ataacgccaa cgcgtcccca attgccagcg
    17641 taatattgca caggcacaca ggactgcaaa cgcctggctg ccgatcatga cggcggtctg
    17701 taaaaaagtg agatgaaggt tccacagatt ggtgcaaaac atcccgcata aaatgggaac
    17761 gacaccacgc cagccaaaga taaacaagat tgacagcatt acgcacagtg gcatccacgc
    17821 cagaaagata taattggaat ggataacagc aagtggcgaa attaaacggg atagctgaat
    17881 aagtaccacg gtgagcgtaa acgctagtgt gaatatcttg atatttttta tcaggttatg
    17941 ctccacaaac atgagtacac tactctcact gatcggggtt ttcaggcaca caaataataa
    18001 gtttattcgc atcgcgaagc aaacaggggc cagcctgttg ctgttttttc agtcagtgat
    18061 gcattcacca cacggcactg ccaggatata gactgtatta caaaagcggc aaaaggcaga
    18121 gacaaaaaac ccccgctttg cagcgagggt ttgaaatttg gtggagctaa gcgggatcga
    18181 accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccacgagt
    18241 tgtttacgta ccaaatttgc tgggtgcaaa atttggtgga gctaagcggg atcgaaccgc
    18301 tgacctcttg catgccatgc aagcgctctc ccagctgagc tatagcccca tcacgtaaag
    18361 cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa cggcaaattg
    18421 ccacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg
    18481 cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa tgaactacta
    18541 atagacccac atacattcag ggaattgtta tgttcaagga gaggatgacg ccagatgaac
    18601 ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc aaggaaggct
    18661 ggacaacgtc accaaaacca ggcgtccagg gtggcaaagc tcgactggtt cacgtcaatg
    18721 aacaggttcg tgaatatatt cgcaatgctg aacgtccaga aggtcaggga gaagcgccag
    18781 ccctttctgg tgatgcgccg cttgaagtgt tgctggtgac actggcgaaa gagatgacgc
    18841 cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc ggattgttac
    18901 aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaataa aatgaccacc
    18961 gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg gatcagagaa
    19021 aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag acttattctg
    19081 gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa cacaccgatg
    19141 cttctggaag ccgaagaacc ccttgccgaa tacgcaccgg gtattcgcac tcctgcttat
    19201 cggcaaatca tcagcgcaat agacaacatg actcacagtg agcaagaaaa agttgcgcaa
    19261 tttttgtcac gcgaaggaat tcgtaatttt cttgctcgtc tcgacataga tgaatcagct
    19321 tgacatcctc cacgccctga atgacgagga tccctgctac gttcaggctg tcgcctgaat
    19381 catttcggtg ggttcctgct tcaacgggcg gcatgactgc accatctctc cacaggcaag
    19441 aacggcgtgc cccgccgcta aaatgttacg agcgccgttt acatcggcgt tcgctgtata
    19501 tccacatatc tggcatctga atttactttg tgacaggcga ttttctttcg ctgtatgacc
    19561 acagcacgcg caacgctggc ttgtgtacgc tggcggaaca gcaagcacct gaccgccacg
    19621 ccagagctgc ttgtactcaa gctggcggcg catttcatac cagccctgat tcagtatcga
    19681 acggtttaaa cctgattttg cccggacatt gcgccccggc tggctgaccg tacccgctgc
    19741 tgactttgac atgttgctga ccttcaaatc ctcaatgact atcattgcgt ggtttttgct
    19801 gacggtcgtt gtgactttgt gaaggtagtc cctgcggata tttgcgatac gggaatgcag
    19861 tcgctgtatt ttgcgtttct gcttctgcca gttattgctg aatttgacct tgcggcttaa
    19921 ctgtcgctga agtctcgcca gcgtcttctg gtttttctga aaactgttta caggcccaaa
    19981 gactgtgcca tctgacagcg tggcgagttt agccacgcca gcatccagcc cgatcattga
    20041 cgctgaaggg tgaaccggag ttgatacttc actttctgtc tgaatactga tgtaccactt
    20101 accgcaggac tggcagacag tgacattttt cacaataccc gtgacctgac ggctgttccg
    20161 gtagcgcatc cagcccagtt tcggcagaaa aatacggctg ttttcctgat cgagcttaac
    20221 gccctgcggg tagcggaatg catcattctg tccccgcttt ttgaatcggg gaaaagcagc
    20281 ccgcttctgg aagaagtttt tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg
    20341 ctgtgagggc gaatctctaa gccattgcgt ttcagtggca tttttccact caaccagcca
    20401 ggaagccatt ttcccgtaag ggatgtattt attaccgacc tcatgattct cattctgacg
    20461 tgccagagca cgattgaaaa cgaaacgaca tgcgcctgcg aagcgcctca tttcacgctc
    20521 ctgttgacca ccgggtctta actggaattt aaatgcctgt agtcgtttca caggtcaaat
    20581 tatattttgg tctgtgaaaa aagaaaccga tatccggcgt ggaaggcatt gtgttttcct
    20641 gatgcatgtt cacctggtct ttgtcccaaa taccgacgcc agatttttga ccatgacgcg
    20701 acagaaaaac tacgcactta ctattcaaat gtatgtgctg attttgaagc tgaacgggtt
    20761 gaaatggatg gcgaaccaga tcacttccat ttgttaatta actatcctcc caaactggcg
    20821 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggtgactgcg acgagatcga
    20881 ccagatattg cagtcaggta ttactacaaa ggcattttgt ggagtcctgg ctattttgcc
    20941 agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
    21001 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggacgggg ttttacggcg
    21061 caccggataa ataaaaaacg gcaggggtat ctctcctgcc gtttatcttt tatgcgctaa
    21121 ttactgctga ttttcgcgtt cagcaataaa agccagcgct ttgttgatac gctcgatact
    21181 gcgggtctta ccgattgcgt gaacggtaac atccagcgcc ggagactgcc ccgcaccggt
    21241 tacggcgaca cgcagcggca taccaacttt acccataccc acttccagct catccgccgt
    21301 cgcctgaata gcgtgatgaa cgttttcagc ggtccagtca gtaatcgcag tcagtttgtc
    21361 acgaaccact tccagcggct gacgcgctac cggacgcaga tgttttttcg cggcgtcggc
    21421 atcgaactca gcaaaatctt cgtagaaata acggcagctc tgtgccatct ctttcagcgt
    21481 cttgcagcgt tcgcccaaca gtttcaccag atcagccagc tgcgggccgt tacgggtatc
    21541 gatgttttcc tgctcaatgt gccactgtaa gtgagtagca acatactccg gcggcaacgc
    21601 gttaatgtag tgatggttca gccacagcag cttgtcggtg ttgaacgcac tggcagattt
    21661 gctgacggca ttcagagtga agtatttaat catctcttca cgagtgaaga tttcctgatc
    21721 gccgtgggac cagcccagac gcaccagata gttcagcagt gcttctggca gataaccatc
    21781 atcacgatac tgcattacgc tgaccgcccc gtgacgtttg gacagttttt taccgtcatc
    21841 gccgttgatc atagaaacgt gcgcgtaaac cggtaccggc gctttcagag ccttaagaat
    21901 gttaatctgg cgcggtgtgt tgttgatatg gtcttcgcca cggataacgt gggtgatttc
    21961 catatcccag tcatcgacaa ccacacagaa gttataggtt ggggaaccat cggtacggcg
    22021 gataatcaga tcatccagct cctggttgct gaactcgatc ggaccacgga tctgatcgtc
    22081 aaaaacaaca gaaccttcct gcgggttagc aaaacgtacg acacacggct catcatcagc
    22141 gtgatgctca tgactgtggc ggcagcgacc gtcataacgc ggcttctcac ctttcgccat
    22201 ttgctcttcg cgcagcgctt ccaggcgctc tttagagcaa tagcatttat aagctgtgcc
    22261 ctcttccagc atctgatcga tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta
    22321 cggaccttca tcccactcca ggttcagcca gttcatgcca tccataatgg cttcgatagc
    22381 ttccggcgtg gaacgctcaa gatcggtgtc ttcaatacgc agcacgaact caccgccgtg
    22441 gttacgtgca aaaagccagg agtaaagagc agtacgcgcg ccgccaacgt gcagatagcc
    22501 tgttgggctt ggcgcgaagc gagttttgat tttcatgaaa tggccttacg tttagaaaga
    22561 tgccgacaac cggcaaatcc tggaaaaatt gagtgggcga tattctatca ctcccgcctg
    22621 attcctcaat gtagttcggg ttgcaacctt acgcattatt gttttcgttg acaaattgcg
    22681 caacacggtc tgttttgcga tcatcttgct taaatttacg acgaacgaac aatttcttta
    22741 gaaaatgcgt tgactcattt tgaactctcc ctataatgcg actccacaca gcgggggtga
    22801 ttagctcatc tgggagagca cctcccttac aaggaggggg tcggcggttc gatcccgtca
    22861 tcacccacca actactttat gtagtctccg ccgtgtagca agaaattgag aagtgggtga
    22921 ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc gatcccgtca
    22981 tcacccacca ctttctcgcc agctagattt cttgtaacaa tgtgaagtac cgaagtgggt
    23041 gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt tcgatcccgt
    23101 catcacccac cacttcgggt cgttagctca gttggtagag cagttgactt ttaatcaatt
    23161 ggtcgcaggt tcgaatcctg cacgacccac caatgtaaaa aagcgcccta aaggcgcttt
    23221 tttgctatct gcaatttgcg aaattgcctg atgcgcttcg cttatcagac caactatgtc
    23281 cggcagttcc cgtgcctctc atctactgcg ccaacgccgc cagcagttta accagcgcgg
    23341 gcgtcacctg ctgcgtttca taaacaatat ataagtctgc cgggatgcgt tctttgagcg
    23401 gacggaatat gacaccgggc cagttcatct gcgcgtagct gtccgctatc aatgtgatac
    23461 caatacccat actgaccatg gcgagtaccg tttgcggttc attaacttcg cgaataacaa
    23521 ccggtgaaaa tcccacctgc tggcaaactc gctgcaaaaa atcccagtca gtgtacacgg
    23581 gcggcattgt aacgaaatac tcgtcacgta gcgcttccag cgggacggtg gaaaatgatg
    23641 agagatgatg ctcttcaggc atcgccacca gaaacgccga ttcatgcaac cgtaagctgg
    23701 taaaaccagt cggtggttct gtcgccattc gccagatccc ggcatcaagt tcgcggcgtt
    23761 ccagcaaggc catttgcatc gcaggcatct tttcgcgaaa cagaacttca acgttaggat
    23821 tttctctaag gaatcgccgc ataaccgggc gcatccgtcc ccacattgcc gttcccacaa
    23881 ccccgagttc aatccgccct gcttctcccc gacctatttg ttcaacccga gccaatacat
    23941 tatttgcatt caccagcaat cgacgcgatt cttccatcaa gattttgccc gcgtgtgtca
    24001 gtacgacgct gcgcgaatgg cgaataaaaa gctgcgtgcc gagttgattt tccagctctt
    24061 taatatgaat actgagcgga ggctgagaca tatttaaacg cgctgctgcg cgaccaaaat
    24121 gcaactcttc cgctacggca agaaaataac ggagcaactt aagatctgtt ctatatacgc
    24181 gttccataat taagtgctcc atataaaaaa tatatcgtat taaattatca tggcataaag
    24241 tatttcacca ccagatatcc gacatacata aacacgatgg ataatatacc cggcaataca
    24301 tttggaacag gaataggtaa tcgcacgatt gtaaacaaag caccaatgcc aaaaccaacg
    24361 ccagaagaaa ataaaatatc tttcactaat taacctttat cataaaagca gccccaagga
    24421 acagagctgc gaatcctttt aatgtgtaac tgaacgatgc gttatctttt cagggtcatg
    24481 gttatattta aacccaaaga aaaatatcac tgcgaggaaa agagcatacc ctgcaaacat
    24541 cagccaaatg gtatgccaat cttttacgcc atccaccgag aagtaatcca ctgccatacc
    24601 gctcagaatc gagccaaccc atgcgccgac gccatttacc atcgtcataa aaaggccctg
    24661 ggcgctggca cgaatgctgg aatcaacttc ctgttcgaca aagaccgaac cagaaatatt
    24721 gaagaaatcg aatgcacaac cgtaaacaat catcgacagc aacagcaaaa taaacccggt
    24781 tggtgatgga tcgccatagg caaagaagcc aaagcgcagc gtccacgcca ccatactcat
    24841 cagcatgacg gttttaatac cgaaacgctt caaaaagaac ggtatcgtca gaatgaaacc
    24901 gacttcagcc atctgtgaaa ctgacagtaa aatggacggg tatttcacca cgaagctatc
    24961 agcaaactca ggattacggg cgaaatcatg caggaacggg ttgccaaaaa cgttggtaat
    25021 ttgcagcacc gcacccagca tcatggcgaa gaggaaaaag atagccatgc gtggattttt
    25081 aaacagcacg aaggcatcca gacccagctt gctggcaagc gatgtagtcg cttttttctc
    25141 cgcaaccgga atcttcggca aagtcagcgc ataagccgac agcagcaatg acgcaccgga
    25201 cgcgatatac agttgcagac tactcaattc cagatgcagc aggcttactg cccacatggc
    25261 gacaatgaac cccaccgtac caaaaacgcg aatgggcggg aaagcggtca ccgggtcaag
    25321 ccctgcctgg gcaagacagg aataagagac gctgttcgat aacgcaatag tcggcataaa
    25381 cgccatcgca ttgactaaca tcacccaaaa catcatatcc ggatcagtta cggatgccgc
    25441 ataaaaaagt acgcccgcac acaccaggtg acacagcatg tatgcacgtt ctgcacgcag
    25501 ccatttgtct gcgatgatcc ccattatacc aggcataata atcgcggcga tccctttgga
    25561 actgtaaacc atgccaacat tagcgccggt gaaatgaagg gtattaatca tgtaagaacc
    25621 gagggtaacc agccagctcc cccagataaa atattgcaaa aaggacatta cctttaagcg
    25681 caaagcgatg ctcataatcg tttccttgtc gcatcatatg gcctcactca gtaaggccgt
    25741 ttattgttgt tttactcagg caattttgcg cagaaagccg caaataagat taataaagtt
    25801 ttgctttgag agttccgctg ctgccagcgt ttgggcatga gaaagcttca cgtcactcag
    25861 accttccgcc atgttggtaa tcgcagagac cgcaacgact ttaagttcgc aatggcgagc
    25921 tgaaataacc tcaggcacca cagacatacc aacaacatcc ccaccaataa tttgcatcat
    25981 gcgaatttcc gccgcagtct cgaaattcgg ccccggatac gagacgaaca cgccctccgt
    26041 cagagggaac ccctcttctt tcgccacttt ttgtaacagt gcgcggtatt ccgcatcgta
    26101 ggcattcgcc agcgagaaga agcgctcacc aaaacgttca tcattaagac ccaccatcgg
    26161 cgttcccggc atggtgttga tgtgatcttt caatgcgacc agactgccgg cccccacttc
    26221 aggacgcagt gagccagccg cattggtgca gaacagcaac tcgcagccca gcaacttaaa
    26281 ggtacggatt gcatccgtca tgatggtcat cccacgacct tcgtagaaat gtccgcgacc
    26341 tttcatgcac gccactggca ccccctggag atacccctgc accagctcac ccgcatgacc
    26401 gtgtacggta cttaccggga acccaggcag cttttcgtag gaaattgcga ccgcgttctc
    26461 aatctgatcg gccagcgcgc ccagcccgga acctaaaata aaggccactc gtggcgtgaa
    26521 atcaggttta taagtcttga taatatctac gcaatacagt gggttatgag aaaaatgagc
    26581 ctgatacata tatatccttt ttttgtaggg tggaatctaa cgccttttta taacgagatt
    26641 gccccacctc ataccaatac acttttttca tgtatccgat agttattcga tatcgatata
    26701 tttata
//
LOCUS       DAZKHK010000073        26664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKHK010000073 DAZKHK010000000
VERSION     DAZKHK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.073"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(73..1476)
                     /locus_tag="ACHOS5_002982"
     CDS             complement(73..1476)
                     /locus_tag="ACHOS5_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFP9777764.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAVAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     MAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            complement(1533..2537)
                     /gene="argF"
                     /locus_tag="ACHOS5_002983"
     CDS             complement(1533..2537)
                     /gene="argF"
                     /locus_tag="ACHOS5_002983"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9777765.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDEALVTQCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            complement(2548..3492)
                     /gene="arcC"
                     /locus_tag="ACHOS5_002984"
     CDS             complement(2548..3492)
                     /gene="arcC"
                     /locus_tag="ACHOS5_002984"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9777766.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKGWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPITTLDPFL"
     gene            complement(3503..4723)
                     /gene="arcA"
                     /locus_tag="ACHOS5_002985"
     CDS             complement(3503..4723)
                     /gene="arcA"
                     /locus_tag="ACHOS5_002985"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFP9777767.1"
                     /translation="MEKHYVGSEIGQLCSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGVEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDVNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
     gene            5401..5853
                     /locus_tag="ACHOS5_002986"
     CDS             5401..5853
                     /locus_tag="ACHOS5_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP9777768.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELRRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKA"
     gene            complement(5898..6902)
                     /gene="argF"
                     /locus_tag="ACHOS5_002987"
     CDS             complement(5898..6902)
                     /gene="argF"
                     /locus_tag="ACHOS5_002987"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9777769.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            7064..7480
                     /gene="rraB"
                     /locus_tag="ACHOS5_002988"
     CDS             7064..7480
                     /gene="rraB"
                     /locus_tag="ACHOS5_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP9777770.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            complement(7657..8160)
                     /gene="yjgM"
                     /locus_tag="ACHOS5_002989"
     CDS             complement(7657..8160)
                     /gene="yjgM"
                     /locus_tag="ACHOS5_002989"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9777771.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            8353..9540
                     /locus_tag="ACHOS5_002990"
     CDS             8353..9540
                     /locus_tag="ACHOS5_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP9777772.1"
                     /translation="MNDVNIGKDNSRHSFVFTGKGGEYFLICLVNFLLTIITLGIYGP
                     WALIKCRRYIYQHVTLKGQPFSYKGTGGAIFVSMLLIVVVYLLSISCFAGQHFALGLF
                     LFALLICGIPCMAVKSLQYQANMTSLNDIRFGFNCSMMRAWWVMLGLPVLLALVFWFA
                     LYLIAQVTTSIGGLFFNLVALSLLSAIGLGVVHGITYSKWMPLLGNNATFGIHKFSIQ
                     VNVKECIKGCMLAILTMVPFIIVIGIMIAPVFQQLMMMTMLGRSDAGSEFVLQYYPQI
                     MASYFLYFVAILVFASYLYVTLRNLFLNNLTLANGTIRFHSSVTAIGMLLRMLAVLMG
                     SSITCGLAYPWLKMWMVSWIANNTHVQGDLDSLELTNDDKPQDSGSLMWISRSIMPYV
                     PFI"
     gene            complement(9587..12442)
                     /gene="valS"
                     /locus_tag="ACHOS5_002991"
     CDS             complement(9587..12442)
                     /gene="valS"
                     /locus_tag="ACHOS5_002991"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9777773.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12442..12885)
                     /gene="holC"
                     /locus_tag="ACHOS5_002992"
     CDS             complement(12442..12885)
                     /gene="holC"
                     /locus_tag="ACHOS5_002992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP9777774.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(13143..14654)
                     /gene="pepA"
                     /locus_tag="ACHOS5_002993"
     CDS             complement(13143..14654)
                     /gene="pepA"
                     /locus_tag="ACHOS5_002993"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP9777775.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            14808..14858
                     /gene="ytgA"
                     /locus_tag="ACHOS5_002994"
     CDS             14808..14858
                     /gene="ytgA"
                     /locus_tag="ACHOS5_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP9777776.1"
                     /translation="MSIIILATISTTQENK"
     gene            14921..16021
                     /gene="lptF"
                     /locus_tag="ACHOS5_002995"
     CDS             14921..16021
                     /gene="lptF"
                     /locus_tag="ACHOS5_002995"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9777777.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            16021..17103
                     /gene="lptG"
                     /locus_tag="ACHOS5_002996"
     CDS             16021..17103
                     /gene="lptG"
                     /locus_tag="ACHOS5_002996"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9777778.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(17264..18766)
                     /gene="yjgR"
                     /locus_tag="ACHOS5_002997"
     CDS             complement(17264..18766)
                     /gene="yjgR"
                     /locus_tag="ACHOS5_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP9777779.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGTIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPRLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQNNPAVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(18844..19842)
                     /gene="idnR"
                     /locus_tag="ACHOS5_002998"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(18844..19842)
                     /gene="idnR"
                     /locus_tag="ACHOS5_002998"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFP9777780.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTIIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERKLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRTAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(19909..21228)
                     /gene="idnT"
                     /locus_tag="ACHOS5_002999"
     CDS             complement(19909..21228)
                     /gene="idnT"
                     /locus_tag="ACHOS5_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFP9777781.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(21293..22057)
                     /gene="idnO"
                     /locus_tag="ACHOS5_003000"
     CDS             complement(21293..22057)
                     /gene="idnO"
                     /locus_tag="ACHOS5_003000"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9777782.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVKKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(22081..23112)
                     /gene="idnD"
                     /locus_tag="ACHOS5_003001"
     CDS             complement(22081..23112)
                     /gene="idnD"
                     /locus_tag="ACHOS5_003001"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFP9777783.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSKLHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            23329..23892
                     /gene="idnK"
                     /locus_tag="ACHOS5_003002"
     CDS             23329..23892
                     /gene="idnK"
                     /locus_tag="ACHOS5_003002"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9777784.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQDDEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(23896..24915)
                     /gene="ahr"
                     /locus_tag="ACHOS5_003003"
                     /pseudo
     CDS             complement(23896..24915)
                     /gene="ahr"
                     /locus_tag="ACHOS5_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
     gene            25111..25195
                     /locus_tag="ACHOS5_003004"
     tRNA            25111..25195
                     /locus_tag="ACHOS5_003004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25145..25147,aa:Leu,seq:caa)
     gene            25382..26644
                     /locus_tag="ACHOS5_003005"
     CDS             25382..26644
                     /locus_tag="ACHOS5_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9777785.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARKLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHSTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEISGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWATELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFCGSHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
BASE COUNT         6611 a         6719 c         6560 g         6774 t
ORIGIN      
        1 tttataatgt gagagtggtt catttatcac tacaccagat ttgaaggccg ctttgataag
       61 cggcctgtcg cgttaaatca tcgcggcgat actcagcaca atcatattca acatcgtgag
      121 aatcagcagt agcggtgcta cccatctcag ataacggacc cagggtacac gggcaatcgc
      181 cagaccgccc atcactaccg cagaggttgg cgtaatcaaa ttaaccaccc cggaagcaga
      241 ctgataagcg gtcaccacca gatcgcgttg tacatgagca aaatcagcga gtggagccat
      301 tattggcatc gttaacactg caaggccgga agatgaggga accagaaaag agagcaatac
      361 ttccagccaa taggtcacgt tgatgaaaac agtggtggaa agccctgaaa ccagattctc
      421 agcactgtgc agaattgtgt gcgtaatcat gccgttatcc atcacgacca caataccgcg
      481 ggcgatgccg ataatcagtg ccacaccaag gagatcccgc gcgccgtcaa taaaggtact
      541 ggtgaaggtt tcttcgccca tgcgggcaat gacacccacg atgaccgcaa ctgccagaaa
      601 gacagcagag atttccgcca tccaccaacc cagtaccgct acgccgtaaa tcataaaggc
      661 aaaggatgca gcaaaaagta tcaggatcgc tttgcgcgtg ggggtaaatt ccagcatcgt
      721 ttctgaacga tttcccagaa aatgcgcctg gttttcagcc atcttatccg ccacgataga
      781 gctttccgga ctgttgcgaa ctttgcgggc ataacgcata acccagtaaa cacaaatcac
      841 gtagcctact aacagcaaaa taacacgcag caaaatgccc tgagtaaagg ggatccctgc
      901 ggcgttggca gcaatgacgg tagcaaatgg gttaatggtt gagcccagcg tgccaatgcc
      961 cgatcccaat aacactgtgg cggcagcgac cagaggatca aagcgggcag ccatcatgac
     1021 gggcaccagc aacgtgtaga aggggagtga ttcttccgcc atcccataaa tagtaccgcc
     1081 agcagcgaag agcgccatca ggatcggaat catccactct tcccgaccat taaggcgagt
     1141 tgtcactcgt tcgataccgg catctattgc cccggtttta tttactactc ccaaaaatcc
     1201 accgatgatc aggacaaaca gcgcgacatc aatcgctccc gcagtgtagg tttgatggtt
     1261 gtacaagccg tcgatgggcg ctaacaggac agccgtaata ccctgtggtt gtgcctcaac
     1321 aggtgcgtaa gtgcctgcaa caggaacttc ctttcccagt gcggcatttg tcaccatctg
     1381 atattttccc gcaggtacaa tccaggtcat tgccgcaact aacgcaatca gcacgaacaa
     1441 gatggtataa gcggtgggaa atttgaattt acccataatg tgatctccat aatcacgggc
     1501 atacgccact cgtcgacgca tgcccgtcag ggttagtcgc cgagagttgc taccatcacc
     1561 gctttaatgg tgtgcatccg gttttctgct tcatcgaaaa caatagagtg ggccgattca
     1621 aaaacctctt cagtaacttc cagtcctttg aggccgtagg ccatctcgat ctcgcgtcct
     1681 actgtggtgt gctcattatg gaaggcgggc aggcagtgca taaatttaac ttcagggtta
     1741 cgcgtggcag taatcacctg ttgattgatc tgataaggtg ccatcagact cactcgttct
     1801 gcccaggctt cttttggctc tcccatcgaa acccaaacat ccgtatagag aaaatcaacg
     1861 tcgtatacgc cttcttccac atcctctgtc agggtgattc tcgccccagt cacgctggcg
     1921 atctcacggc attgtgttac cagcgcttca tctggccaga aagatttagg tgccaccaga
     1981 cggatatcca tccccatttt tgcagcacca accatcagcg agttccccat gttatttcgg
     2041 gcatcaccaa gatacgcaaa gctcagctct ggcagcgttt tgcctggcgc atgttccagc
     2101 atggtcatta aatccgcgag gatctgtgtc gggtgaaatt cattggtcag tccgttccat
     2161 accggaacac cagcgaactc acctaattct tcaacgatat tctgaccata accgcgatac
     2221 tcaataccgt catacatgcg tccgagtacg cgagcggtat ctttcattga ttctttatgg
     2281 ccgatttgtg agccgctggg gccgatgtaa gtcacctgcg caccttgatc aaaggccgcg
     2341 acttcaaagg cacagcgggt acgggtagag gatttttcaa aaatcaacgc aatgttttta
     2401 ccgaccagcg tttgtttttc gtttccagat ttcttggcgg ctttaagatt gatagccaaa
     2461 tcaatcaggt attgaatctc tgctggggtg taatcaagca gcttaagaaa attacggttt
     2521 ttcagtgaag tagccatggg gatgtcctta taaaaatgga tcaagcgtgg ttatgggggt
     2581 atcaaggcga ataagagtgc ctttgtctcc ggcgagaatt tccggaccat ctgcaagaga
     2641 gccgatccct gcaatgcccc ggcactgact aacaaacttt gcgcaggcgg tgactttcgg
     2701 ccccatagac ccggcatcaa actgcatttc gttgagtaat tccggcgtga cctgggccag
     2761 cgggcgttgg gttggcttgc cccagtcgag atatactgcg tccgcatcag tgaggatcaa
     2821 caaggcgtca gcatggatct gacttgccag tagcgcggct gaaagatctt tgtcgataac
     2881 cgcttcaatc ccatggtatc catcggcttt ttcaaccacc ggaacaccac cgccaccatt
     2941 acagatcacc agatgatcgt gggcgatcag tgtctggatg gcatcccgct cgacgatgcg
     3001 tttaggttgt ggagaaggta cgacccggcg gaaagagtga ccatctgctt tgaaaaccca
     3061 gcctttttct gcctgtagta cctgagtctg agcgtggtca taaattgggc cgatgtactt
     3121 agttgggtta ctgaaggcgg gatcgttggc atccacttca acctgcgtca gcagcacgct
     3181 gatttcacgt tgtggtagtt gatttttcag cgcctgttgt agcatgtagc cgatcatccc
     3241 ctggctttcg gcccccagaa tgtcgagtgg atagggagcg acatgggcat aggcgctatt
     3301 ttgaagtgcc agaagtccaa cttgtggacc attcccgtga accagtacaa cccgccagtg
     3361 ttgagtcagt tgggcgatag ttttagccgc aagatcgata tttttacgtt ggatttctgc
     3421 ttcgagtggt tcgcctcgtt taagcagggc attaccgccg agagcgataa ccagtgttgg
     3481 tttgttttcc atgatctctt ccttagatac cgtcacgatg caaagggcaa ctcatgcaac
     3541 gtgcgccgcc acgtccgcgg cctaattcat caccggggat gggtaaaacg gtgatgcctg
     3601 ctttgtcata tttttcgtta gtccagatgt tgcgctcgta gcccactacc actcccggcc
     3661 gaagcgtgag cacattgtta gcgtcattcc actgttcgcg ttctgcctca aaggcatcac
     3721 cccccgtggt gattaaacgt acctggtcaa taccgagggc tttttcgata gcatggagga
     3781 gtgtgctttc ttgagtacgt ttcaggccgc cgtgaccgtc aggtgttagc gtccagcaat
     3841 taacatccgg gcgcacaact tcgggataaa ccgagaaggt gtcgatatcg atatgagtca
     3901 taacggtgtc aagatgcata caggagcggt gtttaggcag ctcaacagcg attacccgtt
     3961 cagcctgacg atgtttaaac aatgcctgag caagaaactc gactccttgt ggcgtggtgc
     4021 gttcagacat gccgatcaac acggcccccc gaccaatcac cagcacatca ccaccttcta
     4081 acgtggcgtg gtcataatta atattctcgt cgccgaaata tttaataaat tcgccgccag
     4141 caaattgtgg atgccagcga taaatagcgc gtagattatt ggtttcacgt tggcgagcag
     4201 gttttgccat tggattaatc gatacaccgt tataaatcca gcatgaggta tcacgcgtaa
     4261 ataaatgatt cggtaacggc ttcataatga aatcattaat atcgtgggta tcaaccacca
     4321 tatttttaat tgaggcggga atttcactat aagtcagccc accacttaaa tgacgagcaa
     4381 ggtcccggtg tgacatctct gcaagccagg tgcggacgtc agtagcaaac gtagggccga
     4441 gtcggtaatc tgatatttgt gtttccagta accagctttt ggcttccggg atatccaatg
     4501 tttgtgttag taaatcagtg agtaacagaa cttctatccc ttgctggcgc agcgtattgg
     4561 cgaaaatatc atgctcttca cctgcccgct caacggacaa tacatcgtca aataacaact
     4621 cctggcaatt ggatggtgtt agacgtttca gactgagatt tgggcgatgc aacataacgc
     4681 tgcatagttg tccaatttca gaaccaacat aatgcttttc catcattatt cctttaacac
     4741 tttttcaaaa ataaagagga gatggctgca aacaaatgaa atgcttgtag tcaggtctgt
     4801 tcagtaattt tcagatgcaa tgattcagtt tatttcttcc agaaaatgtg acggctttca
     4861 caccggcggt gatattaata atcgaagttg ataatatttg atgcggttct gataaatgat
     4921 aaagaaacaa aatacgataa tgcattaata cgcaaatata cgcagagtta ttcttcgttg
     4981 ttaattgatt gttttatttt tcaggaaatg cctttaagta taatgcattg atatttatgt
     5041 atattttaat attcattgca tatctatgcg aaaggataag gtgattattt ttcacagcac
     5101 tattttcacg ggataactat gacagcacgg gcattaaaag ctaagaaaat gaactgtgat
     5161 ctgactgaga gttttctttt aacatatgca ataacaagat attaggacca aaatacatag
     5221 ctaagttata agatttatgc agcaaaactg catacaaaat tactcctgct taacatcagt
     5281 acaacatctc cagcaataaa catgcgcaag ggcaaaacct gatgattttt ctatggtgag
     5341 aggtggcttt ttttcgtata aggaaagaag tgaaacattg ttttaataaa ggaagatatt
     5401 atgattgttg gcaacatcca tcacttacaa tcctggctgc cggaagaact tcgcgaagca
     5461 attgagtaca tcaaatcaca tgtcagcgat gagacagcaa aaggtaagca cgctattgac
     5521 ggcgaccgcc tgttttatct gatttctgag gatacgactg aaccaggtga actgcgtcgt
     5581 gctgagtatc atgctcgtta tctcgatatc cagattgtat taaaagggca ggaagggatg
     5641 acatttagca cgcaaccagc aggcgtacca gagactgact ggctggcaga taaagatatt
     5701 gcttttatcg ggcaggggat cgatgaaaaa acagtgattc ttaatgaagg ggattttgtc
     5761 gttttctatc ctggcgaagt tcacaaaccg ctttgtgcgg tgggagcccc cgctcaggta
     5821 cggaaagcag tagttaagct attaaaagcg taagtgatca ctgccggggg cgccctgtgc
     5881 cgccgcccgg caaaacgtta ttttgctaat gtagcgacca taacagcttt tatggtgtgc
     5941 atccggtttt ctgcctggtc aaagacaata cttgctggag attcaaagac ttcatcggtg
     6001 acttccattc caccatacag accgaattct gcggccattt ttttacccag cgtggtttgc
     6061 tcatcatgaa atgcaggcag acagtggagg aacttaacct gcgaattacc ggtcagggcc
     6121 atcattttgc tattcacctg atagtcacgc agcagggcaa tacgctccgc ccatttctct
     6181 ttgggctcac ccatcgatac ccagacatcg gtatagataa agtctgcgcc tttaacccca
     6241 gaggcgatat cttccgtcaa ggtgatttta cccccgtgtt tttgtgccag agcactgcat
     6301 tcggcgacca gcgcagcttc cggccagcat gcctgtggtg cgaccagacg caagtccaga
     6361 ccggtaagcg ccgcagcttc gagcatcgaa ttgcccatgt tgttacgcgc gtcacctgca
     6421 tagaccagcg tcatttcgtt gaatgctttg ccgggcaaat gctcctgcat ggtgaggaga
     6481 tccgccagca gctgcgtggg gtggaactca ttggtcaggc cgttccatac tggcacgcca
     6541 gcgtattccg ccagtgtttc gacaatctcc tgaccatagc cgcgatactg aataccgtca
     6601 tacatgcgac caagcacgcg ggcagtgtct ttaatcgact ctttgtgacc aatctggctg
     6661 ccgcttgggc cgagataagt aacgcgagca ccctggtcat atgcggcaac ttcgaaagag
     6721 catcgggtac gggtcgagtc tttttcgaag atgagcgcga tgtttttgcc agtgagtctg
     6781 gcttcttctt taccgctttt cttatcggct ttcagcttcg cggctaactg caacaagctg
     6841 ttgagttcag ctggcgtgaa atcgagtaat ttcaggaaat gcttatgata aaacccggac
     6901 atagatccct cctgtggcta acgccttaat gaattaaaat tcaatttata tgtatgatta
     6961 ttcatttgca agtctaaagc ataaatcttt gtcacaaagg tggaggcaat gtcagtggtg
     7021 tgtgacaata agagtatcgg caggacatta agaggaataa gccatggcaa acccggaaca
     7081 actggaagaa cagcgtgaag aaacacgttt gattattgaa gaattactgg aagatggcag
     7141 cgatccggac gcgctgtaca ccatcgaaca ccatctttcc gcagacgatc tggaaaccct
     7201 ggaaaaagca gcagttgaag cgtttaaact cggttacgaa gtgaccgatc cagaagagct
     7261 ggaagttgaa gatggtgaca tcgtgatttg ctgcgacatc ctcagcgagt gtgcgttgaa
     7321 tgccgacctg atcgatgccc aggttgaaca actgatgacg ctggcggaga aatttgacgt
     7381 tgagtacgat gggtggggca cttactttga agatcctaac ggcgaagacg gcgacgatga
     7441 agattttgtc gatgaagacg atgacggtat tcgtcactaa ttaagcgttg acgacaaagg
     7501 ctgcgttacc tgatgcgcta cgcatatcag gcccaggtag ttcctgcaac ctggtgaatt
     7561 tgtagcccgg ataaggcatt tacgtcacat ccagcatact caaaaagcac gtggttaatg
     7621 ttcgaatgcc cgcgagtcta agctcgcggg catttttcaa agtttgcgta gcatccgtac
     7681 ttcacaatcg acatgacccg tgcagccaag cgcatagtcg atatgctcaa agcccaaatg
     7741 ctcataaagc gcaatggctt cctttaaaaa agcggtcgtt tccagatagc agcgtttgaa
     7801 acccatttct cgcgcctgct ccattgccat taaggccagt ttttttgcca gccctttgcc
     7861 gcggatagcc ggaagaaaat acatcttttg cagttcgcaa atgtctgact cactcccggt
     7921 taatggcgca atcccaccgc cgccgaccac ttcaccctcg tactcaacca cccaatatgc
     7981 gtggccaggc tgactataca cttgatacag ctcgtcaagg ttcggatcgg cgacggtgta
     8041 gcctttatca gcggtaagac cgtattcggc ggatacctga cgaatgacgt gggcgatagc
     8101 aggattatcg gcgacggtaa gtttgcgcaa acggagtgcg ggagaagcga caacattcat
     8161 agcatagctc aatttcaaat gtatgattta tagctcactt taataccacc ctcgcttagg
     8221 tggtgcaaga agaagataaa tcagaaaata ttacgcatat ctctctgaga taaagcgtat
     8281 tctaggcgaa ccagtatcaa ttgactggaa ttattatcgg tatgaatgga atttatttta
     8341 gggatatagt tcatgaatga tgtaaacatc gggaaagata attctcggca ctcgtttgtt
     8401 ttcacaggca aaggtggtga gtattttctt atttgtcttg tgaatttttt actgacaatt
     8461 attactctgg gaatatatgg accgtgggca ttgataaaat gccgtcgtta tatttatcaa
     8521 catgtgacac tcaaggggca accatttagt tataagggaa caggtggtgc catttttgtt
     8581 agcatgttac ttattgtggt ggtttatctt ctcagtattt cttgttttgc aggtcaacac
     8641 tttgcgctcg gtttattcct gtttgctctc ttgatctgcg gtattccttg tatggcagtg
     8701 aagagtctgc agtatcaggc gaacatgacc tcactgaatg acattcgctt tggctttaac
     8761 tgctcaatga tgcgtgcatg gtgggttatg ctaggactgc ctgttctgtt ggcgttagtt
     8821 ttttggttcg cactgtatct tattgcgcaa gtgacgacat cgataggagg cttattcttt
     8881 aacttagtcg cattaagttt gctttctgcc atcgggctag gtgttgtcca tggtataacc
     8941 tatagtaaat ggatgccatt gctgggtaat aatgcaactt ttggcattca taagttttct
     9001 attcaggtaa acgtaaaaga atgcattaaa ggttgcatgc tcgcaatatt aaccatggtt
     9061 cctttcatca ttgtgattgg tattatgatc gcaccagtat tccagcagtt aatgatgatg
     9121 accatgttgg gtaggtctga tgctggcagt gagtttgtat tacaatatta cccgcagatt
     9181 atggcttctt atttcctgta ttttgtcgcg atccttgttt ttgccagtta tctctatgtc
     9241 acgttacgca atctgttttt gaataatctg acgctggcaa atgggacaat tcgttttcat
     9301 tcatctgtta ccgcgatcgg tatgcttctg cgcatgttgg cggtattaat ggggtcctca
     9361 attacctgtg gtctggctta tccctggctg aaaatgtgga tggttagctg gattgctaac
     9421 aatacccatg tccagggcga ccttgattcg ctggagttaa ccaatgatga caaaccgcag
     9481 gatagcggtt cgttaatgtg gatatctcgc agtattatgc cgtatgttcc gtttatctaa
     9541 ataaaaaggc cggagcacgc tccggccttc gttttcacta tgagtattac agcgcggcga
     9601 taacagcctg ctgctcaatc agtttcgctt tcgcttccgc ataaccttcc agcttctcac
     9661 gctctttcgc gatgaccgct tccggtgcgc gcgctacaaa gccttcgttc gccagtttgt
     9721 tctcgatgcg gctaatttca ccttcaatct tcgccacttc tttcgccaga cgtgccagct
     9781 catcttcttt gttgatgagg cctgccatcg ggatcagcag ttctgcgccg tcgacgatct
     9841 ttgtaacgga aaccggacct ttatcatcag caggcagcac ggtgatgctt tccagacgcg
     9901 ccagcgtttg caggaagccg cggttttcat ttacgcgacg ttctgcatcc gcgctgcaac
     9961 cacgcagcag cagctccagc ggtttacccg gtgcgatgtt catttctgca cggatgttac
    10021 gtacagcaac gatcgcctgt ttcagccact cggtgtcggc cagtgcggct tcatcaacct
    10081 gagacgcatc gtactgcggg aacggctgca gcatgatggt gtcggcagtg ataccgcaaa
    10141 gtactttcac acgctgccag atggtttcgg tgatgaacgg aatgatcgga tgcgcgaggc
    10201 gcagcagacc ttccagtaca gtcaccagcg tatggcgagt accgcgcagt tcggcttctg
    10261 ttccaccgtt cattaccggc ttggtcagct ccagatacca gtcacagaac tggttccagg
    10321 tgaattcata cagaatgcct gcggcgatat cgaagcggaa gctgtccagc gcttcgcggt
    10381 acgctttgat ggtctggttg aactccgcca gaatccagcg atccgccagc gacagcgtca
    10441 tttcaccgcc gttgaagccg caatcctgac cttccgtgtt catcagcaca aaacggctgg
    10501 cgttccacag cttgttacag aagttacggt agccttccag acgcttcata tcccagttga
    10561 tgtcacgacc ggtagaagcc agcgccgcca gggtgaagcg cagcgcgtca gtaccgtgcg
    10621 gctcaatgcc gttcgggaac tgcttctcgg tgcgcttacg gattttgtcc gccagctgcg
    10681 gctgcatcat attgccggta cgtttttcca gcagttccgg cagcgaaata ccgtcaacca
    10741 tatccagtgg gtcaatcacg ttacctttgg atttggacat cttctggcct tcgtcatcac
    10801 gaatcaggcc ggtcatgtaa acggtgtgga acggcacctg cggtttgcca ttttcatctt
    10861 tgatgaagtg catggtcatc atgatcatgc gggcaatcca gaagaagatg atgtcgaagc
    10921 cggataccat cacgctggtt gggtggaact ggcgcagtgc gtcggtgttt tccggccagc
    10981 cgagcgtgga gaaggtccac agcgcggagg agaaccaggt gtcgagaacg tcttcatcct
    11041 gacgcagcgc aacatcagca ccgaggttat tttctttacg cacttcgtct tcgttgcggc
    11101 caacataaac attacccgct tcgtcatacc atgccgggat acggtgaccc caccatagct
    11161 gacgagagat acaccagtcc tgaatatcgc gcatccagga gaaatacatg ttttcgtact
    11221 gcttcggtac gaactgaatg tcgccgttct caaccgcttc aaccgctggt ttcgccagta
    11281 catcggcacg aacgtaccac tggtcggtca gcatcggttc gataaccacg ccgccacggt
    11341 cgccgtaagg aacggtcagg tcgtgcggtt taatttcttc cagcaggccg agcgcgtcaa
    11401 ccgcagcaac gacggcttta cgtgcagcaa aacgctccag tttctggaac tctgccggga
    11461 tttcgctgga ataaacgtca gattcgttac ctttggtatc gaacacctgg gcgctttcac
    11521 ggatatcgcc gtcaaaggtc aggatgttga tcatcggcag ggcgtgacgt ttaccgactt
    11581 catagtcgtt aaagtcgtgc gctggggtga tcttcacgca gccagtgcct ttttccatgt
    11641 cggcgtgttc gtcgccaacg atcggaatac gacggttaac cagtggcaga atgacatatt
    11701 tgccaatcag atctttataa cgcggatctt ccgggttaac ggccacgcca gtatcgccca
    11761 gcagagtttc cggacgggta gtcgcaacca ccagataatc tttaccgtct gcggttttcg
    11821 cgccgtcagc cagcggatag cggatgtgcc acatcgaacc tttcgattcg cggttttcca
    11881 cttccaggtc ggagatagcg gtgcgcagtt tcgggtccca gtttaccagg cgtttgccac
    11941 ggtaaatcag gtcttcttta tacagacgaa cgaaaacttc tttcaccgca ttggacaggc
    12001 cttcgtccat ggtgaagcgt tcacgctccc agtcgacgga gttgccgaga cggcgcatct
    12061 gacgggtaat ggtgccgccg gattccgctt tccattccca gattttgtcg atgaaagctt
    12121 cgcggccgta gtcgtgacgg gttttacctt cttctgcggc aatcttgcgc tcaacgacca
    12181 tctgggtagc gatcccggcg tggtcagtac cgacctgcca cagggtgttt ttgccctgca
    12241 tgcgctgata gcggatcatg gtatccatga tggtttgctg gaaggcgtga cccatatgca
    12301 aactgccggt gacgttcggc ggcgggatca tgatgcagaa actttcctgg ctttcatcgc
    12361 cattaggctt aaagtagccc tgcttttccc agtgctcgta aagcggctgt tcgatatctt
    12421 gtgggttata tgtcttttcc attatttcca ggttgccgta ttcaggttga aaccagccac
    12481 gcggtaggct ttatagcgtt cgcgcgccag ttgtttcaga gaatcttcat aaggaacgaa
    12541 gtctaccact tctgtgaaag cggtggcaaa atctgcaaag cttgttcgca gactaatcaa
    12601 tatatcgcgc gggctgctgc tgcgcttttg cggccaggcg atctccaccg gcgcaccgcc
    12661 gcgcggtcct tctcccgcta aattatgcgg aacaaagctt tctgccggac gcgcccacag
    12721 ggcttcatcc agtcggtaag cctgcttttc atcttcacag gcgatgagta cgcgcttacc
    12781 actgcgccaa cgttctgcgg caatttcaca caccaactgc tcaacggcgc ttaagccatc
    12841 gacggtggtg tcattgtcca gaaggtagaa cgttgcgttt ttcatatatg gggcttcttg
    12901 tggtggattt aaatacgcat caggtataca ccgcagcgcc tgatgcgacg ctgccgcgtc
    12961 ttatcaggcc tacaagttaa gtgctgtgtg taggtcggat aaggcgttca cgccgcatcc
    13021 gacaataaca gccttgcctg atgcgacgct gtcgcgtctc atcaggccta cgagttcagt
    13081 gctgtgtagg tcggataagg cgttcacgcc gcatccgaca ataacaacac agcctgatgc
    13141 aattactctt cgccgttaaa cccggcgcgg tttagcagga actgtgccag caacgccacc
    13201 gggcgaccgg ttgcgccttt cgccttacca gaacgccagg cagtaccggc gatatccagg
    13261 tgcgcccagt tatacttacg ggtaaagcgc gacaggaagc aacctgcggt aatcgcccca
    13321 ccaggacgac cgccaatgtt cgccatatcg gcaaaattgg actccagctg ttcctgatac
    13381 tcgtcaccca gcggcaaacg ccatgcgcgg tcaccggatt gttcagatgc ggcaatcagt
    13441 tcatgggcca gcggattgtg gttcgccatc agaccggtaa tgtgatggcc tagcgcgata
    13501 acgcaggcac cggtcagcgt cgccacgtca atcaccgctt ccggctcaaa ccgctcaacg
    13561 taagttaata cgtcgcacag taccaggcgg ccttcagcgt cggtattcag cacttcaacg
    13621 gtttgaccgg acatggtggt taacacatcg cccggacgat aggcacgacc gccaggcatg
    13681 ttttcgcagc ctgccagcac gccgataacg ttaatcggca gttgcagctc cgcgaccatc
    13741 cgcatcacac cgtaaaccgc tgccgcaccg cacatatcgt acttcatctc atccatgcct
    13801 tctgaaggct tgatcgagat accgccggag tcgaaggtta aacctttacc caccagcacg
    13861 attgggcgtg catcttccga cgcgttgcct ttgtactcaa tcaccgacat cagcgattcg
    13921 ttttgtgaac cctgaccgac cgccagataa gagtgcattc ccagctcttt catctgctgt
    13981 tcgccgataa cgcgggtgat gacattcttg ctgtagctgt cagccagctg gcgcgcctga
    14041 gaggcgagat aagcggcgtt acagatattc ggcggcatat taccgagatc tttcgctgct
    14101 tttatcccgg cggcaatcgc cagaccgtgc tggatcgcgc gctcaccgct ggtcagttca
    14161 cggcgggtcg gcacgttgaa caccatctta cgcagcgggc gacgcggttc gctcttattc
    14221 gttttcaact gatcgaaact gtagagcgtc tcttttgctg tctcgacagc ctgacgcact
    14281 ttccagtagt tgttacggcc tttaacgtgc agttcagtca gaaagcagac cgcttccatc
    14341 gagccagtat cattcagcgt attaatggtt ttctgaataa cctgcttgta ctgacgctca
    14401 tccagctcac gttctttgcc gcaaccaata aggagaattc gctcggaaag tacattcgga
    14461 acatggtgca gcaacagtgt ctgccccggt tttccttcca gttcgccccg acgtagcagg
    14521 gcgctgatgt acccatcgct gattttatcg agctgttctg caatcggaga aaggcgacgt
    14581 ggttcgaaga cgcccacgac gatgcaggca ctccgctgtt tctccgggct accgcttttt
    14641 acactaaact ccatgcacta cgctcctgaa tcttaaagac aacggcggtg gctacagata
    14701 gaattgcaag ctttcgtaac tcatgtccgc tgttgcgatg acttcgtgtt aatcttaacg
    14761 ttattacggc attggcacgt cagaacaaaa tctgagaggt gaatccgttg agtataatta
    14821 tcttagcgac gatttcgacg actcaagaga ataaatgacg tttaagccat gaaacaagct
    14881 aaaatcctgc aaaagacgag tttttacggg cgtatttaaa gtgataatca taagatatct
    14941 ggtgcgggag acgctcaaaa gccagctggc gatactcttc atcttgcttt tgatcttctt
    15001 ctgtcaaaag ttagtgagga tcctcggcgc agcggttgac ggcgatattc cggcgaatct
    15061 ggtgctctcc cttctcgggt tgggcgtgcc ggaaatggcg cagcttatcc tgccattaag
    15121 cctgttcctc gggctgctga tgacgctggg caaactgtat accgaaagtg aaattacggt
    15181 aatgcatgcc tgcggcctga gcaaagcggt tctggtgaaa gcggcaatga tccttgcggt
    15241 attcacggca atcgtcgcgg cggttaacgt gatgtgggcg ggaccgtggt catcgcgtca
    15301 tcaggatgaa gtgttagcag aagcgaaagc gaaccctggc atggcggcgc tggcgcaagg
    15361 gcaattccag caagcgacta atggcagctc ggtgctgttc atcgaaagcg ttgacggcag
    15421 cgatttcaaa gatgtgttcc tcgcgcaaat tcgaccaaaa ggtaatgcac gtccttctgt
    15481 ggtggtggcc gattccggac atttaaccca gctgcgcgac ggctcccagg tcgtcactct
    15541 caaccaggga acgcgcttcg aaggcactgc actgttacgt gatttccgca ttacggattt
    15601 ccaggattat caggcgatca ttggtcacca ggcggtggcg ctcgacccga acgataccga
    15661 ccagatggac atgcgtacat tgtggaacac tgacaccgat cgtgcccgcg cagaactgaa
    15721 ctggcgtatc acgttggtat tcaccgtgtt tatgatggca cttatggtcg taccgttgag
    15781 cgtggttaac ccgcgtcagg gacgcgtact gtcgatgctg ccagccatgc tgctgtacct
    15841 acttttcttc ctgatccaga cctccctgaa atcgaacggc ggtaaaggta agctggaccc
    15901 gacgctgtgg atgtggaccg ttaacctgat ttatctggct ttggcgattg ttctcaacct
    15961 ttgggacacc gtgccggtcc gccgcctgcg cgccagtttt tcgcgtaaag gagcggtgtg
    16021 atgcaacctt ttggcgtact tgaccgctat atcggtaaaa ctattttcac caccatcatg
    16081 atgacgctgt tcatgctggt gtcgctgtcg ggcattatca agtttgtcga tcagctgaaa
    16141 aaagccgggc aggggagtta cgacgcgtta ggcgcaggaa tgtatacctt gctgagcgtg
    16201 ccgaaagatg tgcaaatctt cttcccgatg gcggctctgc ttggggcgtt gcttggtctt
    16261 gggatgctgg cgcagcgcag cgaactggtg gtgatgcagg cttctggttt cacccgtatg
    16321 caggtggcgc tgtcggtgat gaaaaccgcc attccgctgg tcttactgac gatggcgatt
    16381 ggtgaatggg tcgcgccgca gggcgagcag atggcgcgta actaccgtgc gcaggcgatg
    16441 tacggcggct cgttgctctc tacccagcaa ggtttatggg cgaaagatgg caacaacttc
    16501 gtctacattg agcgggttaa aggtgacgaa gagttaggtg gcatcagcat ttatgccttt
    16561 aacgagaatc gtcgtctgca atccgtacgc tatgccgcta ctgcgaagtt tgacccggaa
    16621 cataaagtct ggcgtctgtc gcaggttgat gaatctgatc tgaccaatcc gaaacagatt
    16681 accggttcgc agacggtgag cggcacctgg aaaaccaacc tcacgcctga caaactgggc
    16741 gtggtggcgc tggacccgga tgcactctct atcagcggtt tgcacaacta tgtgaagtat
    16801 ctgaagtcga gcggtcagga tgccggacgt tatcagctca acatgtggag caaaatcttc
    16861 cagccgctat ctgtggcggt gatgatgctg atggcgctgt cgttcatctt tggcccactg
    16921 cgtagcgtac cgatgggcgt gcgtgtggtc accggtatta gcttcggttt tgtcttttac
    16981 gtactggacc agatcttcgg tccgctgacg ttggtttatg gcatcccgcc gatcatcggc
    17041 gcactgttgc caagcgccag cttcttctta atcagcctgt ggctgttaat gagaaaatcg
    17101 taaccgaatg aacgcataaa gggagcgaaa gctcccttta ttattgttag caaagtgtgc
    17161 ttcgttcatt cctgaaaaat aattaaaatt caatatattg ctgtggggtg ggcatgataa
    17221 gacgcgccag cgtcgcatca ggcaaagaca aatctcgcct gacttacctt cttctccccc
    17281 ccagcaaact ccccaacatc ccgcgtacaa tctgattcgt cacctggcga gcggcgcttt
    17341 tggccattgt ttgcaccaca ccatctttct tcccgccgcg tggtccggta gtgccaaaca
    17401 aaatatcctt caatccacca agaatgccgt catccaccgc cacctctttc cctttcacgg
    17461 caggattatt ttgctgctcg gtgcttgcct gtacgccttt ttgcaacatc tcataggcgg
    17521 attcccggtc cacgtcatcc tcgtacttgc catacaccgg agagtgatta atcaagccat
    17581 tacgctcatc ttccgtcacc ggtcccatcc gcgaacaagg cgcaatcacc atcgcccgtt
    17641 ccaccacaga aggacttcct tttgcatcga gaaacgagat taacgcctcg ccggtcccca
    17701 gttcctggat tgccttttcg gtatcaaatg ccggattggc ccgcatggtt tgcgccgctg
    17761 ccttcactgc tttctgatct tttggcgtaa aagcccgcaa agcgtgctga acgcgattac
    17821 cgagctgccc gagcacatta tccggaatat cagacgggtt ttgcgaaacg aaccagacgc
    17881 ccacgccttt tgagcgaata agtcttatca cctgctcaat cttatccagc agtacctgcg
    17941 gtgcatcgtt aaacagcaga tgtgcttcgt cgaagaaaaa caccagtctt ggtttctcca
    18001 gatcgcccgc ttccggcaat tgttcataca actctgaaag catccacagc aggctggcgg
    18061 cgtacagttt cggcatctga taaagtttct cggcgctgag gatattgata acgcctttac
    18121 cgttggcatc ggtgcgcatc cagtctttga tatccagcat cggctcacca aagaagtgtg
    18181 ctgcgccttg ctgttccagc gacaacagcc cgcgctggat ggtaccaacc gatgcgctac
    18241 tgatatttcc gtactgattc tggaaagatt tggcgttatc gccgatgtac tgggtaatcg
    18301 cccgcaaatc tttaaagtcg agcagcaaca atccctgatc gtcagcaata cggaagatga
    18361 tattcagcac gccagattgc acatcgttga gattcaacag ccgcgccagc aacagcggcc
    18421 ccaggtctga aaccgtcgcc cgcaccggat ggcctttctc gccaaagata tcccacacca
    18481 ccaccggatt ggcatgcggt tgccaatcat tgacgccgat atttttaagc cttgcgagca
    18541 gtttttccga cgccgttcct gcctgcgcga taccggtcag atcgcctttc acatcagcca
    18601 taaacaccgg cacgccgatt tccgacaatg actctgccag tttttgcagc gtaacggttt
    18661 tacctgtccc cgttgcgcca gtaatcagtc cgtgacggtt agccattccc ggcagtaaaa
    18721 aaagttctgt gtccggcgtg cgggcaatta acaggggttc gctcatgaga tttcctccag
    18781 tttcctgcct ggagtatagg caacgtccgg caaagagaat acgtgtaatg aagagaaaat
    18841 aacctaaagc gtgttgccgt gataaatctg atacccgagg tcaacagtgt tgtggttgtg
    18901 atcgttattt ttaattttgc tcagcaacat ttgggcagcc gtccgaccaa tatcaaaacg
    18961 gggagtaatt acgctggcga ggcttgggat catttgcctg ccaatctcca gcccatgaaa
    19021 acccgcaatg gaaatttgtt ctggcacggc gagttttcgc tcgcggcaca gtagaagcgc
    19081 acccatcgca atatcgtcat tggtgcaaaa tacaccgtct aaatccgggt ttgcgctaag
    19141 ggcatcacgc attaactgca ttcctaaatg aatggatgag atggcgcgtg gattcatgcg
    19201 taatggggaa agattatgca gcatcatcgc atcgcaatac ccctgataac gctgttcatc
    19261 gcgggtgtca tctttggaac cgagatacag aattttgtgc ctgactcgct tctccagcat
    19321 ggtacacacc atgtcaaaag cggcctgccg attatcaaaa ccgacctcca tatccagccg
    19381 ttctccctgt acatccatca attccacgac cggaatggtg gctgaacgca gaaatttcac
    19441 cgtcctgatg gtgtgatatt tttccgaaag tataatcccg tcaatgttat aagagagcag
    19501 gttaatcacc gactcttctt cggaatcgcg atcgtaattg taattagcaa taatggtctg
    19561 ataattatgc acagaggtga cggattctat tcccgcgaga atgtcagcga agagttgatt
    19621 ttgaaatgac ggaatcaaga taccgagggt ataactttgt gcattcaaca acatgccggg
    19681 agcgcgatta gggatataat tgatctcttc cataattttg gcgatgcgct cgcctgtttc
    19741 ctttgccacc tttttcggcg agcggatata acgactcacg gtcatttttg ttacgccagc
    19801 cagcgtagcg atatcctgta aagaaattct gtgattgcgc ataacttctc ccaacgtcgg
    19861 gggaatcttc aatcttcccc cgaaactaca tcacaatatt ttattctttc agtgcaatac
    19921 ggcgttaatg gctaacacgc ccagcaaacc cataatagaa ataagggttt ccatcaccgt
    19981 ccaggtacgc aacgtttcac caaccgtcag attaaaatac cctttaaata gccagaagcc
    20041 agggtcgttt acgtgtgacg cgatcacgct gcccgcaccg gttgccagta ccattaaagc
    20101 gggatcggca tgggtaacgt tgataatcgg caacaccaca cccgcggtgg taatggccgc
    20161 gacggtggca gagcccagag cgatacgcaa cagcgccgcg acagtccagc acatcaataa
    20221 cggcgaaagt gtagttccgg tcattaagtg tgaaatatac tgcccgacac cgctatctac
    20281 taatacctgc ttaaacgcgc cgccgccagc gataataaac acaatcatcg cgatagcgcc
    20341 tatagaatcc ccaatgatat ccatgatttg ctcgatggtg cgtccattac gtcggcccag
    20401 tgtgaaaatc gcaataacaa tggcaataaa cagcgcaacg gcagggttac cgacaaattc
    20461 aaagaagagg cgcacggtgt tagttttcgg taacgtaatt tcacaaacgg cggcgatagc
    20521 catcaggatg accgggatca cggcagcgaa aatactgttc cagaaggagg gcatctcctc
    20581 ttcgctaaac agatgaggat taaataagcc ttccggtggt gctttctcaa agcgagttag
    20641 cagtttagaa aacagcggtc ctgcgacaat aactgtcgga atggtaatga taaatccata
    20701 cagtaaagtc gttccgagat tagcctcaaa gatagtcgcg atggcagtag gccctggatg
    20761 tggcggcaga aaacagtggg ttacagagag cgctgctacc attggtacgc caacatacaa
    20821 caggggtaat cctgatgatg ctacgatggt aaataccaac ggcaacagca ggacaaaacc
    20881 cacttcaaaa aacatggcga ggcccacaac cagaccggtg atcactagcg cccattgcac
    20941 gcgtttttta ccaaaagtag caatcagcgt agtggcgata cgttgtgccg cacccgtatc
    21001 agaaatcagc ttgcctaaca tggccccgaa accgaggatc attgccagcc cgccgagcgt
    21061 gctgccgata ccattttgta tagagtgcag gacggcctgt gcatccatcc cttcggcaaa
    21121 tccgacgacg gcagctacca gaacgagggc aataaagccg ttaactttaa agccgatcat
    21181 caggataaga agcagcgcga cgcctgccgc aataatgatt aatggcatag tctttatctc
    21241 ttattagtgc cagaaaagca gcgctttcgc gctgcacgta gggtagtagg tgttaaacag
    21301 ccactaacat gccgccatca acaaacaaca ggtggccgtt tacgaaatca gaggcttttg
    21361 aagaaaggaa caccgcagca ccaatcagtt cctgcggatc tccccagcgt gcggcggggg
    21421 tccgtttgca caaccaggcg gtgaaggctt cgtcctcaac cagtgcttta gtcatttctg
    21481 ttttgaaata gcccggcgca ataccgttga cctgaatatt gtggcgcgcc agctcgacgc
    21541 acatgccgcg ggtgagcatt tttaccgccc ctttcgatgc ggcataaggg gtgatggtgt
    21601 cacgtcccag ttcgctttgc atcgagcaaa tattaataac tttacctgcc ttgcgttcaa
    21661 ccatgtgacg agttaccgct tgcgacacca ggaacacggc ggtctggttt actgcgatca
    21721 catcattcca ctcttgttca gggaactcag taaaaggatg acggcgctgg ataccggcgt
    21781 tattcaccag cacatcaatg gggccgatgt ccttttcgat atgttcaacg gcggcatcaa
    21841 tttcatgttt atgagtaaca ttaaaaggtg cggcaacggc ctgaataccc tcctggtgga
    21901 gtttttttac agcaagttct gcgcgttcgg cagtaatatc attaataatt atttgtgcgc
    21961 catatttacc caggccggtt gccagtaaaa agccaatgcc ctgtgctgaa ccggtaatca
    22021 agatattttt tcctgccagt gaaaatagat cgttcattgt attattcctt tatttatctc
    22081 ttagaaaaca agctggactt ttgctgcctg ggttttatca ccggcgaaac gtagcgcctc
    22141 ttccaggtca gtgaagggat attcagcact cagtaaaggc agtggattga taacgccatt
    22201 cgccagccat gacactgcgg tattaaattc gctggtaaaa cggaaagagc ctttgagtga
    22261 aatctcctta ccaatcaatg tcatcattgg gaattctgcc atcgcgcctc ccatccctac
    22321 ctgcaccatt acgccgcgtg cacgagtgac ctccagacag gtattcactg atgaaggatg
    22381 accggacact tcaaagctga catcgaaata gcctttttcc gctttccagt gatccatgtc
    22441 gtcgttttgt gggtttacga gcacatccgc ccccatctct ttgcccagtg aaagggaacg
    22501 gggactcaca tcggcacaga caatttccgc ggcccccagt gttttcacag cactgacaat
    22561 caggcagcca atgggcccaa caccagaaat aaatactcgc ttgccctgta actcgccggc
    22621 ctgatgtgcg gcatgaatcg cgacggctaa aggttcggca aaagccataa ccttttcgtc
    22681 agctttggcc ggataaggga cacattgcga cgtttcgacc attttataac gggtaaagcc
    22741 accatcaaca tgagggaaat acatggcact gccaaaaaaa cgcatctctg tacactgatt
    22801 ctcgttatgt tcaatgcagt atttgcagtg accgcacggt ttagacggat taatggctac
    22861 cgtttgccct tcatgtaatt ttgatgagtc gctatgaata actttaccga taacttcatg
    22921 acctaacacc atcggtgcct ttatcatgaa attacctact tttccttcct gataataatg
    22981 taaatcggaa ccgcaaattc cacctcgggt tatttgtact aatgttccat tattattcca
    23041 atctatcgtc tgctcggtaa cggcaacagt tttcttgccc gcaacaacgc aggactgtgt
    23101 tttcacttgc ataaattcct cactggtcag gtagacacct cggaagcatt taagcggttt
    23161 taattgtcat tatttgtgat gaagatcacg ttagaaaatt gttacattac gatgttacgc
    23221 ataacgtgat gtgccttgta attcttatca gcagaaataa aaaaacgtga aattattatg
    23281 ccaccaggcg tagtatcgca gcaggtaaga tgattcagga gattttaaat ggcgggtgaa
    23341 agctttattt tgatgggcgt ttcagggagt ggtaaaacat taattggtag caaggttgcc
    23401 gcgttattat ctgctaaatt tattgatggt gacgatcttc atccagccaa aaatatagat
    23461 aaaatgtcgc agggtattcc attatctgat gaagatcgac ttccctggct ggaacgctta
    23521 aatgatgctt catacagtct ttataaaaag aatgaaacag gatttattgt ctgttcatca
    23581 ttaaaaaaac agtatcgtga tattttacgc aagggtagcc cccatgttca tttcctctgg
    23641 ttagatggcg actatgaaac tattctcgcg cgaatgcagc gtcgggctgg gcattttatg
    23701 ccggtagcgt tactaaaaag tcagtttgag gcactggagc gtccacaaga cgatgaacag
    23761 gatattgtgc gcattgatat caaccatgat attgccaatg tcaccgaaca atgtcggcag
    23821 gctgtgctgg cgatacgaca aaacagaata tgtgcgaaag agggcagcgc ctcagatcag
    23881 cgctgcgaat gattttcaaa aatcggcttt caacaccacg cggtaacgcg ccttaccgtc
    23941 gcgcacatgc tggatggcgt cgttaatttt cgacatcggg aacagttcgg tagtcggcgc
    24001 aaccttgctg cgggcggcaa agcgcatcag cttacgcaac tcataaggtg taccggtagc
    24061 agagccagag acactgcgat cgcccgcaat cagcgtaaag gccggaaccg gcaacggcgt
    24121 gagaaccgca ccgacggtgt ggaaattacc gccatacgtc agcgcctcaa aataaggcta
    24181 ccagtcgagg ctgacgttca cagtattgat aatgagatca aactgccccg ccagagtttt
    24241 cagtgcctgc ggatcgcggc tattcaccac tttatcggca cccatcgcca gtacttcctg
    24301 ctctttcgcc ggatttgaac taaaggctgt cacctcgcat cccattgcgt gcagaagttt
    24361 tatagcgata tgtcccagcc cgccaatacc aattacccca acgcggctgg tagcagtgat
    24421 atggtgcatc aacagtggtt taaagaccgt gataccacca cacagcagcg gcccggcgga
    24481 ctctatatcg atattttccg gcagtgggat cacccattgc cagtctgcac gcaacttctc
    24541 ggcaaaaccg ccgcgattca taattgttgg caccgcacct tgctcgcagt tgatctgatt
    24601 accgctaata caggcatcgc aatgcccgca gctacgcgcc gtccagccaa tcccgacacg
    24661 ctgaccgacc tgcaaacctt tatcctgcgc ggcgctcccg agcgccacca cgcgaccaat
    24721 cacctcatgc ccggcaacca gcggatattg tgaaaatccc cattcgttat cgatcatcga
    24781 cagatcggaa tggcagatcc cgcagtaatc cacctgcact tcaacatctt gtggcttcag
    24841 ttcaccggga tcgtactcat aaacttccag ttcgccgcct gcttcttttg cggcatagct
    24901 ttttatcatc gacatttttt ggtccttctc tagtgttgtc tgggaagtgt agagcatggc
    24961 agggcggggt gcctggagtg tgacaaaggt tacacatcgc tgtatgcaat gctgaaaatt
    25021 tcagcactta gcgaggtgcg agcaagctgg cgcttgcatg gtggcgtgcg acaggtataa
    25081 tccacaacgt tttccgcata cctcttcagt gccgaagtgg cgaaatcggt agacgcagtt
    25141 gattcaaaat caaccgtaga aatacgtgcc ggttcgagtc cggccttcgg caccaaaagt
    25201 atgtaaatag acctcaactg aggtcttttt ttatgtctga aatccagtat ttatctacct
    25261 ttcccgctat attaactctc tcaaggtcaa cccatatcaa cgtacatcta ccaacacatg
    25321 ttggtacaga tgatggtatt tccggttcga taatgcttgt accaacaggg agggaatacg
    25381 catggcatta acagatatca aagtcagaac agccaagcca acagataagc aatataagct
    25441 gactgatggt ggcggtatgc atctgcttgt ccatccaaat ggttctaagt actggcgttt
    25501 gcagtaccgt tatgagggaa agcaaaagat gttggcactt ggggtttatc ctgaaatcac
    25561 actagcggat gccagagtac gccgtgacga ggcgcgtaag ctgcttgcga atggagtcga
    25621 tccgggagac aaaaagaaaa atgataaggt tgaacagagt aaagcacgaa cctttaaaga
    25681 agtcgcgatc gagtggcata gcaccaataa aaagtggtct gaagatcacg cccatcgtgt
    25741 gctaaaaagt ctggaagata atctttttgc agcgcttggt gaacgtaata tcgctgagtt
    25801 aaaaactcga gatttattag cacccattaa ggccgtggag atctctggac gtcttgaagt
    25861 ggcagctcgt cttcaacagc gcactacagc catcatgcgc tatgcagtac aaagtgggtt
    25921 aattgattat aacccggcac aagagatggc tggggcggtt gcttcctgta atcgacaaca
    25981 tcgtccggcg cttgaattaa agcgcatccc tgagttgctt acaaaaatag atagctatac
    26041 tggtaggccg ctaacccgat gggcgacaga actcactttg ctgatcttta ttcggtccag
    26101 tgagctgcgt tttgctcgct ggtcagagat cgatttcgaa gcgtctatat ggactatccc
    26161 accggagcgg gagcctattc ctggagtgaa gcattcccat agaggctcaa aaatgcgtac
    26221 aacgcatcta gtgcctcttt caacgcaagc ccttgcaatt ttaaagcaga taaaacagtt
    26281 ttgtgggtct catgacttga tatttattgg tgatcacgat tcgcacaagc ctatgagtga
    26341 aaatacggta aatagtgcgt tacgggtcat ggggtatgat acaaaagttg aggtttgtgg
    26401 tcatggcttc cgcacaatgg cctgtagttc attgatcgaa tcaggtttgt ggtctcgtga
    26461 tgctgttgaa cgtcagatga gccacatgga gcgaaattca gtgagggccg cgtatatcca
    26521 taaagcagaa catctggaag aacggcgact gatgctacag tggtgggcag attttctgga
    26581 tgcgaacaga gaacaatgta tcagcccgtt tgaatatgca aagattaaca atccattaaa
    26641 atagtaatca tcccgggctg gcag
//
LOCUS       DAZKHK010000074        26519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKHK010000074 DAZKHK010000000
VERSION     DAZKHK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.074"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..418
                     /gene="hdeA"
                     /locus_tag="ACHOS5_003006"
     CDS             86..418
                     /gene="hdeA"
                     /locus_tag="ACHOS5_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP9777786.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            534..860
                     /gene="hdeB"
                     /locus_tag="ACHOS5_003007"
     CDS             534..860
                     /gene="hdeB"
                     /locus_tag="ACHOS5_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP9777787.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            924..1571
                     /gene="yhiD"
                     /locus_tag="ACHOS5_003008"
     CDS             924..1571
                     /gene="yhiD"
                     /locus_tag="ACHOS5_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9777788.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1623..2393)
                     /locus_tag="ACHOS5_003009"
     CDS             complement(1623..2393)
                     /locus_tag="ACHOS5_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFP9777789.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(2390..3382)
                     /gene="shuU"
                     /locus_tag="ACHOS5_003010"
                     /gene_synonym="chuU"
     CDS             complement(2390..3382)
                     /gene="shuU"
                     /locus_tag="ACHOS5_003010"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFP9777790.1"
                     /translation="MLKDSLSSARIFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3431..4054)
                     /gene="chuY"
                     /locus_tag="ACHOS5_003011"
     CDS             complement(3431..4054)
                     /gene="chuY"
                     /locus_tag="ACHOS5_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFP9777791.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHANAAMKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGITRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(4054..4548)
                     /gene="hutX"
                     /locus_tag="ACHOS5_003012"
     CDS             complement(4054..4548)
                     /gene="hutX"
                     /locus_tag="ACHOS5_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFP9777792.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(4561..5898)
                     /gene="hutW"
                     /locus_tag="ACHOS5_003013"
     CDS             complement(4561..5898)
                     /gene="hutW"
                     /locus_tag="ACHOS5_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFP9777793.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(5918..6832)
                     /gene="chuT"
                     /locus_tag="ACHOS5_003014"
     CDS             complement(5918..6832)
                     /gene="chuT"
                     /locus_tag="ACHOS5_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HFP9777794.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRVQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7516..9498
                     /gene="chuA"
                     /locus_tag="ACHOS5_003015"
                     /gene_synonym="shuA"
     CDS             7516..9498
                     /gene="chuA"
                     /locus_tag="ACHOS5_003015"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFP9777795.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9547..10575
                     /gene="chuS"
                     /locus_tag="ACHOS5_003016"
     CDS             9547..10575
                     /gene="chuS"
                     /locus_tag="ACHOS5_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFP9777796.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MCGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(10632..11162)
                     /gene="dctR"
                     /locus_tag="ACHOS5_003017"
     CDS             complement(10632..11162)
                     /gene="dctR"
                     /locus_tag="ACHOS5_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9777797.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(11315..11881)
                     /gene="slp"
                     /locus_tag="ACHOS5_003018"
     CDS             complement(11315..11881)
                     /gene="slp"
                     /locus_tag="ACHOS5_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP9777798.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGTFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12071..12451)
                     /locus_tag="ACHOS5_003019"
                     /pseudo
     CDS             complement(12071..12451)
                     /locus_tag="ACHOS5_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13067..13492)
                     /gene="arsC"
                     /locus_tag="ACHOS5_003020"
     CDS             complement(13067..13492)
                     /gene="arsC"
                     /locus_tag="ACHOS5_003020"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP9777799.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEKLGLAEDKFTHDQLIDFMLQHPILINRPIVVTPQG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            13827..13910
                     /gene="dinQ"
                     /locus_tag="ACHOS5_003021"
     CDS             13827..13910
                     /gene="dinQ"
                     /locus_tag="ACHOS5_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP9777800.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(13964..15316)
                     /gene="gorA"
                     /locus_tag="ACHOS5_003022"
     CDS             complement(13964..15316)
                     /gene="gorA"
                     /locus_tag="ACHOS5_003022"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9777801.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDKTVAIHPTAAEEFVTMR
                     "
     gene            complement(15388..16230)
                     /gene="rlmJ"
                     /locus_tag="ACHOS5_003023"
     CDS             complement(15388..16230)
                     /gene="rlmJ"
                     /locus_tag="ACHOS5_003023"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP9777802.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            16433..18475
                     /gene="prlC"
                     /locus_tag="ACHOS5_003024"
     CDS             16433..18475
                     /gene="prlC"
                     /locus_tag="ACHOS5_003024"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP9777803.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENDELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            18483..19235
                     /gene="rsmJ"
                     /locus_tag="ACHOS5_003025"
     CDS             18483..19235
                     /gene="rsmJ"
                     /locus_tag="ACHOS5_003025"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP9777804.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(19284..20753)
                     /gene="dtpB"
                     /locus_tag="ACHOS5_003026"
     CDS             complement(19284..20753)
                     /gene="dtpB"
                     /locus_tag="ACHOS5_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP9777805.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            20919..20956
                     /locus_tag="ACHOS5_003027"
                     /pseudo
     CDS             20919..20956
                     /locus_tag="ACHOS5_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="frameshifted; too short; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21070..21504)
                     /gene="uspA"
                     /locus_tag="ACHOS5_003028"
     CDS             complement(21070..21504)
                     /gene="uspA"
                     /locus_tag="ACHOS5_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP9777806.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            21895..22230
                     /gene="uspB"
                     /locus_tag="ACHOS5_003029"
     CDS             21895..22230
                     /gene="uspB"
                     /locus_tag="ACHOS5_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP9777807.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(22301..23800)
                     /gene="pitA"
                     /locus_tag="ACHOS5_003030"
     CDS             complement(22301..23800)
                     /gene="pitA"
                     /locus_tag="ACHOS5_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP9777808.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            24032..25225
                     /gene="yhiN"
                     /locus_tag="ACHOS5_003031"
     CDS             24032..25225
                     /gene="yhiN"
                     /locus_tag="ACHOS5_003031"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9777809.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(25312..26364)
                     /gene="yhiM"
                     /locus_tag="ACHOS5_003032"
     CDS             complement(25312..26364)
                     /gene="yhiM"
                     /locus_tag="ACHOS5_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFP9777810.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGYGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         6845 a         6385 c         6732 g         6557 t
ORIGIN      
        1 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
       61 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
      121 gcttcttctg ccagtagtga gcaatgcagc ggatgcacaa aaagcagctg ataacaaaaa
      181 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
      241 tgcagttggt tttgctgaag cactgaacaa caaagataaa ccagaagatg cggttttaga
      301 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaaa
      361 agccagcttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
      421 ccgggaatgc gttacatcgt atttccttgc atattgaaca ggccggaata tcttctttaa
      481 aagcagctat tcctcctgtt tgtatataat ctctatattg aatgggttac aaaatgaaca
      541 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
      601 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
      661 ttgatctgaa tccaaaagca atgacaccag ttgcgtggtg gatgctgcat gaagaaacag
      721 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
      781 tgatcgaata ctgcaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
      841 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
      901 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgactt atacttgcgg
      961 taattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     1021 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     1081 ttgctgacgt gctgtcttta ggtcacgtcg gactggaccc cagccgtatt gctgctcagg
     1141 ttgtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     1201 taggtctgac gacggcagcg gatatctggg tgaccgccgc cattggtatg gttattggca
     1261 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgctggtg ctggaagtct
     1321 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
     1381 acggtaatac ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa acggatttag
     1441 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
     1501 cgacgtcgat agaagatctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
     1561 caattagcta atctgtatag ttatttattc gcaggagtac cagtgcggta ctcctgttgg
     1621 ttttaatgat cgaggacgat cagtggtgaa tggttggcag gatctttaag tacggtaata
     1681 tccgcgccgt agagcattgt cagcgcctgc tgtgtcaata cgtcctgcgg cttaccattt
     1741 gcgataacct ttcctttttg cattaataca acacggtctg cataacgcgc ggcaagattt
     1801 agatcgtgca atacgcagca aacattgaac tgccgttcgt gaactaactg gcgtaataaa
     1861 cgaaacaagt gctgctgatg gtgaatatcc agcgccgaag ttggttcatc aagaaataac
     1921 catttcggtg agggagtagg ttcccagagt tgcactagca ggcgcgctaa ttgcacgcgc
     1981 tgttgttcac caccggataa ttgccgatag tcgcggtttg ctaacgcctg acagtcacat
     2041 aacgccatta tttgtgctgt ttcattatcc tgattaccag tacgatgcgg atgccgcccc
     2101 atctggataa cttcttgtac gctgaatgga aacgccatgt ggctattttg acgcatcacg
     2161 gcccggtgtt tcgccagttc agtaatcgac cattcattta atggcttatt aaacaaccga
     2221 cattcgccgc tatcaggttg aagatagccg gttagctggc gaagcagcgt agattttccc
     2281 gcaccattag ggccgaggat ggcaacaatt tccccaccgg gaaaagtcag cgagacatta
     2341 tcagtcagac gccgcccctg aaggctataa accagattct gggcgctgat catgtgatcc
     2401 tccggttgcg cagaatcaac cacataaaat aagggccacc aagcaggctg gttaataatc
     2461 ccacgggcat ttctgctggc tgtaccagcg tgcgggcaag cgtgtctgcc atcagcaata
     2521 aacaggctcc ggcgagggcg gaacaaggga ttagccagcg gtgatttgcc ccggtagtca
     2581 tgcgaatcag atgcgggatc accaggccaa taaagccgat aataccgctt accgatacgg
     2641 cagcaccaac gagcagtgag ctcactaaca gtaattgctg gcgtttacgc ttcacgttca
     2701 cgccgaggta gtgggcttct tcatcaccga gctgcagtaa attcagcgtt ccggcgagac
     2761 aagttgttgc gataatggcc ggtaggatga atgaactggc aaccaataac gtcggccatt
     2821 gcgcctgtcc aagattgccc atcatccaca atgtgagttg tcgtagctgc tgctcatcgc
     2881 cgatatagct caggatgccg atcgctgcgc cgcacagggc attgatggcg ataccggcga
     2941 gtaataattg catcatgctg ccatcgcgat ggcgctgcgt gatgagaaaa atgatggtgc
     3001 agaccactaa acttccggca atggcaaaca ccatttgctc atagagcacc agcaccactg
     3061 ggaaggagaa gggcagcaca atagcaacgc caaccattaa tgcagaaccg ctactgacgc
     3121 caagtaatcc agggtcagcc attgggttgc gaaaaagccc ctgcataatt acccctgcgg
     3181 ttgccaacgc tgcacctacc agcaccgcca gcaaaacccg tggtagacga atattgagcc
     3241 agatatcgcg gtattcctca tcaaaaaggg catcaaaact gatctttaac gctccctgac
     3301 tggcaccaaa cagcaccaga gctagcaata atagtgatag ccccataaag atcctggcgg
     3361 aagagagtga atccttgagc acgcaaaact ccgttacgac attacggcgc ggacataccg
     3421 cgccggggga tcacgccggt ttcaggtccg gttcaatcag actgtagacc tgctggttaa
     3481 gtgctggcgc atttgccagt tcatggatat gtgcggcgac atcagcacgg ttaacaaaac
     3541 catgacactc ctgattttgt atccgttgtg ctttgcccgt tgccgcacca tcaagcagac
     3601 cgccagggcg gagaatggca taatcaagct ggctggtttg caaccagctt tccgccagcg
     3661 ttttttcccg taccgcctga ccaaacgcag cctttgcccg ctctgataaa aacggccagc
     3721 tatcgccgca tcccagtgac gtcaccagaa tcatgcgtgt gatgcctgct ttctcggcct
     3781 cgtcaatcac tgttctgtgt gccagataat cctgagctcc gcccattgtt gagatgataa
     3841 gcgcatccgg gccagctgcg cggcaagcag ccgcgactac gctggcatca caggcgtcgc
     3901 ctgtaaaaac ctgtacgcct tgttgcgcca gtttcatggc ggcattggca tgacgaatga
     3961 cagcaacgac cggacgttgt tccgccagcg ccagttcaag cgttctggca cccacacctt
     4021 ttccgcctgc gccaaagagt agccacggtg tcatcaggcg tgttccttta acgatgctgc
     4081 cagagcatgg aaggcgctga cttgttcgct caggagctga cgatgatcat ccctaccgag
     4141 gaagattttc agcattgcgc tgccttcctt attgaaaaag agaatagagg cggtgtccat
     4201 gcccataaat ttacgttcga tcaaggcaat atgcgtgcag ttttctgctt tgatatgccc
     4261 ggacatgccg tgctttccgc gtaggttaaa atagccgtgg cgatggaaac ccgaaggcag
     4321 ttcgccgcta aattcgagta ttacatcggt agtatgtact aacgtggtga cgttgcccca
     4381 ttcgcagacg gtatcccaga cagtatcgaa tttatcacct gacacaacag tcggagaggg
     4441 cagatttttc actacctcca gcagcgtggt gttgtattgt tcggcgatcg cctccagcgt
     4501 accgtctggc tccgttttca aaaattcctg taacgagaca tggctcataa atcactcctg
     4561 ttatggtttt tcaagcgcaa ttgctggcgc attaagtacc tgaatcagtt cattaagaga
     4621 ctgcaaaatg ttgcttgccc agaaacgacc ttcattggtc aaccgcagac acgttgacgc
     4681 atcgcggctt aagccttttt ggtgccactg ggccagtaac ggcgcgagtt tttcggcatg
     4741 tggcgtaagt tcgtccagcg gtacacgcgc cgtttcaatg cccgactgca aggtatgacg
     4801 ccattgcgca ttacgttcgg cgttacgcat gatcatcatc agcggttttt tgcctgccgc
     4861 gacggattca tgccaggtct gaagattgcg ttcgttcatc caggagtacc cattaatcga
     4921 accgccggct ccggaaccaa aggccagaca atcggcacct tgtttaatca gcaggttata
     4981 gagattgcgt tcgcgtgtgg tacggcccca gtggctgtta ctgatgcagc gccaaccggc
     5041 atcgtccata aaatcacatc cttgcaggta aagatcgcga cgttctgccg gagagggcac
     5101 ggtagtacgc ccattttcca cggctttgcc cagcggtgta ttgggcagga cattgagcgc
     5161 atagagatcg acgccgtcga gaccgatatc gcgggcaata gccagatctt ctccccaggt
     5221 ttgcgcatcc tgacccggca gaccaaacag caggtcacag accaccgcgg cacggtcgcg
     5281 tttgaccagg ctttccataa acgcaatagc ggttgggcca tccgaggtgc gggccatttt
     5341 cttgcggatt ttgctgttaa acgactgaat accaattgag aagcggtttg ctcctgcatc
     5401 aaggcaagca tcgattcgct cagcgtcaaa attcagtacc cggccttcga tagtgatttc
     5461 acaatcaggg gccagtggca gcttttctcg taatgtggtg atgattctgg ccaaatcgtg
     5521 tgctgaaagc gcggaaggcg taccgccacc gaaatagacc gcgtggatgg gggcagactg
     5581 atgcaatacg ctgtctgctt ccatttctat ttcacgaatc agggcgtcgg tataatgagc
     5641 acacgcatcg tcattaaaac ggttctgata aaaaccacaa aacgtgcagt gtgtcgcaca
     5701 aaaaggaatg tgtaggtaaa ccagtcgctt acgtggtgat accgtttgat tgatcatctc
     5761 ttgccaggtc tgtgccagtt gctctttggc aaccggaatg gccccacgaa acggcatcat
     5821 ggcgcgcctg tctttaaagg gctgatcacc gtctaaagca aaatgtggcg tgagatccag
     5881 ggtgttattt gtgttcattc gtctgattta aatccattta ttgtggccaa agctgctggt
     5941 gtaaagactc aacgacatca gcaatacgcg gacccattcc cagaatcagg ttttgatcaa
     6001 cggtgataat gcgctggttt ttccatgcgg cagtgtgggt aattccggca atagaacgta
     6061 gccgattaat atcgccatcg accatttgtg aggtgacgac aatcacttca gggttagccg
     6121 caatcagcga ttccgcgctg tagcttttgt actgctggtg ggtagcgacg ttttctgctc
     6181 cggcaagcga aagaatggcg tccgcgacgc tgcctttacc ggcaacctgt ggcgcacttc
     6241 cgcctgcgga gagaataaac atggctttaa ccggggcttt tttggccgcc acgttttgct
     6301 gtactcgctc caggcgttgg ttgatctgtg tcaccagcgc ctcgccttgt tcaggaacct
     6361 gtaacgtttt tgccagttgg cgaatgttgg cgtacatctg ctcaagggtg gctggtacac
     6421 gcggcagagt gacgacgttg actttttgca cccgcagttg gtcgagcaca atttgcggtc
     6481 ctgcatcctg ccaggtaatt acgctatccg ggcgaagtga caaaatgcct tcgctgctga
     6541 gttgtttcca gtaaccaata tgcggcagtt tggcggtttc tggtggataa gatgtcgttt
     6601 catcgacacc aaccacgcgt ttgccagcgc ccatcgcgta gattaactcc gtcagcgatc
     6661 ctcctgcgac cacgatacgt tcggcagccg ttacgctaaa agtccagcta agagccagta
     6721 atgccgtaaa caattttttt cgttttacca tggtcttcgc ggcagagacc acggctgaaa
     6781 tacataacgt tagtggggaa aaaagaaacg gcctggtgat gatccttggc ataatgtgag
     6841 aactcttatc aaattgagaa tgatcatgat tatcatttga gttatatatg aaatacaacc
     6901 gtcgattcaa tacgcacggc gttacaagcg tatttatttt tgatttttat caagatgctt
     6961 cgtcagctaa tgttcttgtt accgattaaa tttcagcgcg ggaaaaagct gagaacccgg
     7021 agatatcgcg gcttgcagag caaagagaag agaaaattat cactgttaac gacaacgttt
     7081 tgtgatgagg ttgtgggggg atagccataa acacaggatg gttttctgga tgctggatag
     7141 atttcataac cccctgttta ttgtgttaat ggcggttaaa aaaagcttga tcataaaaaa
     7201 ttgatcgata ataattctca ttatcacgtt tgagtttaac aaaatatgac atcatggata
     7261 acaaaatcat atcttttgta agggaaaacg cacagcactc ttcacttcag acgtggcgta
     7321 ctactttgcc tgacaaagag ggcgtgcttt tgctgaatta tcagaaatat tcggcaattt
     7381 tacgggatat atacgctaat agcttcccgt ggtgatatct aatcatttga attgtaaata
     7441 aatttatttt tcgccagcca ccccggtatt aacccaggca gccagcattt tttattttcc
     7501 tcatggagaa tcgctatgtc acgtccgcaa tttacctcgt tgcgtttgag tttgttggct
     7561 ttggctgttt ctgccacctt gccaacgttt gcttttgcta ctgaaaccat gaccgttacg
     7621 gcaacgggga atgcacgtag ttccttcgaa gcgcctatga tggtcagcgt tatcgacact
     7681 tccgctcctg aaaatcaaac tgctacttca gccactgatt tgctgcgtca tgttcctgga
     7741 attactcttg atggtaccgg acgaaccaac ggtcaggatg taaatatgcg tggctatgat
     7801 catcgcggcg tgctggttct tgtcgatggt gttcgccagg gaacggatac cggacacctg
     7861 aatggcactt ttctcgatcc ggcgctgatc aagcgtgttg agattgttcg cggaccttca
     7921 gcattactgt atggcagtgg cgcgctgggt ggagtgatct cctacgatac ggtcgatgca
     7981 aaagatttat tgcaggaagg acaaagcagt ggttttcgtg tctttggtac tggcggcacg
     8041 ggggaccata gcctgggatt aggcgcgagc gcgtttgggc gaactgaaaa tctggatggt
     8101 attgtggcct ggtccagtcg cgatcggggt gatttacgcc agagcaatgg tgaaaccgcg
     8161 ccgaatgacg agtccattaa taacatgctg gcgaaaggga cctggcaaat tgattcagcc
     8221 cagtctctga gcggtttagt gcgttactac aacaacgacg cgcgtgaacc aaaaaatccg
     8281 cagaccgttg aagcttctga tagcagcaac ccgatggtcg atcgttcaac aattcaacgc
     8341 gatgcgcagc tttcttataa actcgccccg cagggtaacg actggttaaa tgcagatgca
     8401 aaaatttact ggtcggaagt ccgtattaat gcgcaaaaca cggggagttc cggcgagtat
     8461 cgtgaacaga taacaaaagg agcaaggctg gagaaccgtt ccactctatt tgccgacagt
     8521 ttcgcttctc acttactgac atatggcggt gagtattatc gtcaggaaca acatccgggt
     8581 ggcgcgacga cgggcttccc gcaagcaaaa atcgatttta gctctggctg gctacaagat
     8641 gagatcacct tacgcgatct gccgattacc ctgcttggcg gaacccgcta tgacagttat
     8701 cgcggtagca gcgacggcta taaagatgtt gatgccgaca aatggtcatc tcgtgcgggg
     8761 atgactatca acccgaccaa ctggctgatg ttatttggct catatgctca ggcattccgc
     8821 gccccgacga tgggcgaaat gtataacgat tctaaacact tctcgattgg tcgcttctat
     8881 accaactatt gggtgccaaa cccgaactta cgtccggaaa ctaacgaaac tcaggagtac
     8941 ggttttgggc tgcgttttga tgacctgatg ttgtccaatg atgctctgga atttaaagcc
     9001 agctactttg ataccaaagc gaaagattat atctccacga ccgtcgattt cgcggcggcg
     9061 acaactatgt cgtataacgt cccgaacgcc aaaatctggg gctgggatgt gatgactaaa
     9121 tataccactg atctgtttag ccttgatgtg gcctataacc gtacccgcgg caaagacacc
     9181 gataccgggg aatatatctc cagcattaac ccggataccg ttaccagtac cctgaatatt
     9241 ccgatcgctc acagtggctt ctctgttggt tgggtcggta cgtttgccga tcgctcaaca
     9301 catatcagca gcagctacag caaacaacct ggctatggtg tgaatgattt ctacgtcagt
     9361 tatcaagggc agcaggcgct caaaggcatg accactactc tggtattggg caacgccttc
     9421 gataaagagt actggtcgcc gcaaggcatc ccacaggatg gtcgtaacgg aaaaattttc
     9481 gtgagttatc aatggtaatc atctgccccg atatttcggg gcatttatct ggaaggaaga
     9541 gagacaatga accactacac acgctggctt gagttaaaag aacaaaatcc cggaaagtac
     9601 gcgcgtgaca tcgcaggttt aatgaatatc agcgaagcag aactggcatt tgcacgcgtc
     9661 acgcacgatg cgtggcggat gtgcggcgat atccgtgaaa ttctggcggc gctcgaaagt
     9721 gttggcgaga ccaaatgtat ttgccgtaat gaatacgcag tccatgaaca agttggtgcg
     9781 ttcacaaacc agcatttgaa cggacatgcc ggattgatcc tcaatccgcg cgcgctggat
     9841 ttacgtctgt ttctcaatca atgggccagt gttttccaca tcaaagaaaa cacggctcgt
     9901 ggcgaacgcc agagtattca gttctttgat catcagggcg atgcattact aaaagtttat
     9961 gccaccgaca ataccgatat ggcggcatgg agtgagcttc tggcacggtt tatcaccgat
    10021 gagaatacgc cgcttgagtt aaaagccgtt gatgcgccag ttgttcaaac gcgagccgat
    10081 gccagtgtgg tcgagcaaga gtggcgggcg atgaccgacg ttcatcagtt ttttacgttg
    10141 ctcaagcgcc acaacctgac gcgccaacag gcgttcaatc tggtggcaga tgatttggcc
    10201 tgcaaagtat ccaacagtgc gttggcgcaa attcttgaat ctgcacagca ggatggcaat
    10261 gaaatcatgg tgtttgttgg caaccgtggc tgcgtacaga ttttcaccgg tgtggtagaa
    10321 aaagtggtgc caatgaaagg ttggctgaat attttcaacc cgacgtttac ccttcatcta
    10381 ttagaagaga gcattgctga agcctgggtt acccgtaaac cgaccagcga tggccacgta
    10441 accagcctgg aattgtttgc ccatgatggt acgcagatag cgcaacttta tggtcaacgt
    10501 acagaaggtg aacaggagca agcgcaatgg cgtaagcaaa ttgcttcgtt gataccggaa
    10561 ggcgttactg cataacgtat aattaacggg aggtctggct gttgctgggc ctctttatat
    10621 tgttcaatcg ttcacaccag ataattaata tgctgatggc gtaccagctc attgatccgc
    10681 ttaagattga ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt
    10741 ttcagtttct ccagaatatc ttccgttgtc atttgattcg caatacagct cagaacctga
    10801 tgctgcttgc gcgagaggga cacatccgct tcaggcacag agcagtaact gccattgatg
    10861 agtgcgacaa agtcgatgat ggcgatagtt cgcggaacaa aggtcaccgg tactttcccc
    10921 aggcagcgtt taatattaaa gggggagaaa ataattacat ggacaggttt taatcgttca
    10981 atttgagtga gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag
    11041 gcattctgat gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct
    11101 ttactcagaa tgtttttcat cgcggtgaaa aacatcgtat ccctggtaat tataagaaac
    11161 ataaccgact cctggcacgg acattaactt tataataagt ttgccaaagg taattaacag
    11221 gacagggctt ataataatag ttagcttact gtgtattaat ttgatgtaat gcatgatgca
    11281 gatgctgaga aagcatctgc atctttcggt ggtgttattt gaccagctca ggtgttacct
    11341 gactcaccgc attggtgtag taaggagccc cccagcccgg ttccggccag aatgtaccat
    11401 agccgtaatc ccacaagtta taggtggtat taaccacttc tctcaaatgc cacacctgga
    11461 tgccctgcat attcacttcc aggaagttat acgggacttt attgataaag ccaggttgtt
    11521 caccctgaat ggtaccgagg atggtaacaa agtgattacg atagttcact ggatcaagga
    11581 agccgctttg tctggcgagc agtcggccct gatagttggc ttcaatatcg ggcttcgcat
    11641 agctatccag cggtaataca gcgatttcta acaacgtatc cgttttacca ttgataacgt
    11701 tgataacctt cccgccaaag cgcgcttgtt gaccaacata taaccccggc tggttatgaa
    11761 cagcaacaaa acttttttga atatcaggtt ggttattgcc tttgatattt tgcggaattg
    11821 aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt gtcatgttca
    11881 tgttactatc cttatcaaca aatattaaaa ccataataga tatttcttta tagttttcat
    11941 ctgattctga ggtgatatgc ctgatagtaa cgtaaacctg tctgatgttt atatctcttc
    12001 tgtttagttg agctcagtta attttggggc gtggcatgag tatccattgt tccataatct
    12061 atattcagag gtttttaatc ttttcgtgtc taatgttatt tatctgatta agtattgatt
    12121 gtgtcttttg cggaagtggt gtcgagctta tcgtttcaat gttattaata agacttacaa
    12181 gttgagcctc aactttgagt agtctgcatt tgtatatgtg gtgttcgtat ggcacgcatt
    12241 aatttgttgg cgttgttgtt gcgctaaatc aaattgttca gaggtgtatc cattctccag
    12301 agaatgggga attaatgggt tgttgttctt cccggttatc acaaggccac ttcggtgtgt
    12361 ctggcgtttt aacattatcg gtatttgttg tgcagttata aatatcacca ttatatgtat
    12421 ttattacacc tggggtaaag tcaattgaca taacattcct tattcatcat ttgatttatg
    12481 ctaagatttt taaattggat aattagaaca ggaatggatt ttcttctaca tacagtaaat
    12541 ataatgcatc atatcttcct gatataatta actttgactt ccattttatt tccatctggt
    12601 aatatttgag tgcaaaaaag gcaataatag tatcgcaaaa gaaaacgtta acaactatcg
    12661 aaaatgctcg ttttaaaaag ttatgatgtt ctctgatagt gtgtgaagtg atatttttgt
    12721 gatgttttag atatgcaata ataaaatggg ttaacgtatg ttgagattat ttattgtgta
    12781 aaccactgat agatcataat ccagtacatc tttacagtgc tgcactgtat cgctgataac
    12841 taagcagctc actgatacac catgctggac gcgataattg gaatctcacg cagggagtac
    12901 tgtgtaaata gcaaaattgc ctgatgcgct acgactgtca ggcctacaaa taacatacaa
    12961 ttcatcgaat tatcataacg ttgtaggtcg gataagacgc gccagcgtct tatccgacat
    13021 aaataaagcg catttttcta acaacctgtg aggggatata gccgctttat ttcaggcgat
    13081 tacccgcctc atcaactact ttctcgccat cttccttggt gaacgcgcct ttttgcgcat
    13141 ctggcagaat ttccagcacg acttctgaag ggcggcacag gcgagttccc tgtggcgtca
    13201 ccacaatcgg gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aactgatcgt
    13261 gagtaaattt atcttctgca aggcccagtt tctcatacgg ctcgacgttt ttacgcagca
    13321 acgcgcgtac ggtaatcccc atatcggcaa tgagttttac cagctcatcg cgcgtcggtg
    13381 gtgtttccag ataatagata atggtcggtt ctgtaccgct gttgcggatc atctccagcg
    13441 tattacgcga cgtaccgcag gccgggttgt gataaatggt aatgttgctc atatcagtat
    13501 ctcattacga tgtgaaagag aggtgtagcg ccagcgccac cagcgttaca aaccaaagcg
    13561 gacaagtcaa aaacacatat ggtttaacga atatgtaagt gtagagaaag gtgtaggtgt
    13621 gaattaagcg ttgggcattc atttgaaagg aggtctgaat caggacgtaa aaaaaccgac
    13681 ttcgcgtcgg tttttttact gttcagccct gagttggtgg ctctggctgg agtgagatag
    13741 cccacatcta acaatgagac atgcgccgtg acaggcaatg gatgagtaag cggatgcatt
    13801 ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt cttggggcgt
    13861 taattgcgct gctggaactg atccgctttc tgcttcagct tctgaactga tagcggaaac
    13921 gtagctaagg gctaagagca cactactctt agccctttaa catttaacgc attgtcacga
    13981 actcttctgc cgctgtcggg tgaatggcaa ccgttttgtc gaagtctttt ttggttgcac
    14041 ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaaa ccaatgccgt
    14101 gaataccgac aatcttctct tccggtccaa cgcataccag tttcatgcgg cacggctggc
    14161 ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact ttcacctgat
    14221 cgtcgccgta ctgctcgcgc gcctgcggct ccgttaaacc aacagtacca atcggcggat
    14281 ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc ttgttattaa
    14341 acaggcgttc agagagacgg cgacctgctg caacagccac cggtgtcagt tccactgcac
    14401 ccgtgttatc gcccaccgcg taaatacctt caacgttggt gttttgatat ttatcgacga
    14461 cgatatagcc tttttcgttg gttttaacgc cagcggcttc caggttgatg ttgtcattgg
    14521 caggctcgcg accaatcgcc caaattaggc aatccaccgt ttcactgcga ccatcttcca
    14581 gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg gcgttggtgt
    14641 gcaactgcgg gccttcggcg ttcatcactt caaccagcgt ttcagagatc atcgggtcga
    14701 agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt cttcgcaccg aggccgttaa
    14761 tcacgcccgc cagttcaacg gcgatgtaac ccgcgccaac aaccgctaca cgctctggca
    14821 aagccggaag ggcgaagaag ccatcagaat caataccgta ttccacgcct ggaatatccg
    14881 ggtggctcgg acgaccaccg gtggcgatca gaatatgatc ggccgtgatg gtttcgccgt
    14941 tgacttcaat ggttttggca tcaacgaagc gggcaaagcc tttgattaca tcaacgttat
    15001 ttttaccgag cacgttctca taggaagtat gaatacggtc gatataggcg gtacggctgg
    15061 cgatcaacgt ttcccagttg aatttattga tagtggtatc aaaaccataa tccgggccgt
    15121 acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc ggcacacagc
    15181 caacatttac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat ttctggccgt
    15241 acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg gcgatgtaat
    15301 catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg tagcgcgaga
    15361 cgtaataggt gccagcaatg gctgcaatta ctctggcacg atccagctta cggtggcgtg
    15421 cccggtgcct gccggaacca gtttgctgtg cagccacggc agcacgttat tcatctgctg
    15481 ttccagcttc cacggcgggt taatcacaat catgccagaa gcggtcatgc cacggcgatc
    15541 gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg tcgcttccag
    15601 gtcgtggatc atgcgcttaa tttgctggcg cagcaccact ggataccaca atgcgtaagt
    15661 gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct gatagtcggt
    15721 tttcatttca tacggcgggt cgataaggat taaaccacgg cgggaaaccg gcggcagctt
    15781 ggccttaagc tgctggaaac cgtcggcttt ttcgacgcgc gcacggctgt ctttctgaaa
    15841 ttcagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca ggctgtcctg
    15901 ttcacgcagt agctggcgag caatcaacgg cgaaccgggg taataacgca actgcccgct
    15961 acggttgaag tgttttacca cattgatgta cgcctccagc tccgcaggca aatcgtcctg
    16021 ctgccagata cgggcgatgc cttcgagata ttcgccggta cgctcggcat gttcgctgcc
    16081 taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt tatctttctc
    16141 tttcagcgac tcaatgatca ggctctgaac ggtatgttta aggacgtcgg cgtggttgcc
    16201 agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa gtaaaaatcg
    16261 gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga ggccgccgcc
    16321 gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct acaaacatta
    16381 tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc taatgacgaa
    16441 tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg aacatgtcgt
    16501 tccagccgtg actaaggcgc tgaacgactg ccgcgaaaat gtggagcgcg tagtagcgca
    16561 aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg acgatgtgct
    16621 ggggcgtatc ttctccccgg tcagccatct gaactcggtg aaaaatagcc cggaactgcg
    16681 tgaagcgtac gaacaaaccc tgccgctgct gtcagaatac agcacctggg tagggcaaca
    16741 tgaagggctg tataaggcat atcgcgacct gcgcgatggc gatcattacg ccacgctgaa
    16801 cacggcgcag aaaaaagcgg ttgataacgc actgcgcgac ttcgaactct ctggcatcgg
    16861 tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt ctgaactggg
    16921 caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac tcgttaccga
    16981 cgaagcggag ctggcgggga tgccagaaag cgcgctggct gcggcaaaag cccaggctga
    17041 agcgaaagag ctggaaggtt atttgctgac gctggatatc ccaagctacc tgccggtaat
    17101 gacctactgc gacaaccagg ccctgcgtga agagatgtat cgcgcttata gcactcgtgc
    17161 ttccgatcaa ggtccgaacg ccggtaagtg ggacaacagc aaggtgatgg aagagatcct
    17221 cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct tcaaatcact
    17281 tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg atctggcaaa
    17341 acgcgcgcgc ccgcaaggcg aaaaagagct ggcgcaactg cgcgccttcg ccaaagccga
    17401 atttggcgtc gatgagttgc aaccgtggga tatcgcttac tacagcgaaa aacagaaaca
    17461 gcacctctac agcatcagcg atgaacaact gcgtccgtac ttcccggaaa acaaagcggt
    17521 taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag agcgtaaaga
    17581 tgttgatgtc tggcatccgg atgtacgttt cttcgagctg tatgacgaga acgacgaact
    17641 gcgcggcagc ttctacctcg acctgtatgc ccgtgaaaac aaacgcggcg gggcgtggat
    17701 ggatgactgc gtaggccaga tgcgtaaagc cgacggttcg ctgcaaaaac cggtcgcgta
    17761 tctgacctgc aacttcaacc gcccggtaaa tggtaaaccg gcgctgttta cccatgacga
    17821 agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga cccgcattga
    17881 aaccgctggt gtttccggga tcagtggggt gccgtgggat gcggtcgaac tgccgagcca
    17941 gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctccg gtcactatga
    18001 aaccggcgag ccgctgccga aggagctgct ggataaaatg ctggcggcga agaactacca
    18061 ggcggcgctg tttattctgc gccagctgga gttcggcctg ttcgatttcc gcctccatgc
    18121 agagttccgt ccggatcagg gggcgaaaat cctcgaaact ctggcagaaa tcaagaaact
    18181 ggttgccgtg gtaccgtctc cgtcatgggg ccgtttcccg catgctttca gccacatttt
    18241 tgccggtggt tatgcggcag gttactacag ctacctgtgg gccgatgtgc tggcggcaga
    18301 tgccttctcg cgttttgagg aagagggcat tttcaaccgt gaaactgggc aatcgttcct
    18361 cgacaacatt ctgagccgtg gcggttcaga agagccgatg gagctgttca aacgcttccg
    18421 tggtcgtgaa ccacagctgg atgcgatgct ggagcattac ggcattaagg gctgaacatt
    18481 cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta tctgttctgg
    18541 cggctcgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg ttaacgccgg
    18601 agcacctgga actgcgcaag cgtgatgagc cgaagctggg cggcatcttt gttgattttg
    18661 ttggcggggc gatggcgcac cgacgcaaat tcggcggtgg tcgcggagag gcggtggcga
    18721 aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc gcaggattag
    18781 ggcgcgatgc ctttgtgctg gcttcggtcg gctgccgcgt gcggatgctg gagcgtaatc
    18841 cagtggttgc cgcgctgctc gacgatggcc tggcgcgtgg ttatgcggat gcggaaatcg
    18901 gcggctggtt acaggaacgt ttgcagttga ttcacgcctc cagtctgacg gcgctgagcg
    18961 atatcacccc gcgtccgcag gtagtttatc ttgacccaat gttcccgcat aagcagaaaa
    19021 gcgcgctggt gaaaaaagag atgcgcgtgt ttcagtcgct ggtggggccg gatcttgatg
    19081 ccgatggatt gttggagcct gcgcggttgc tggcaaccaa acgcgtggtg gtgaagcgtc
    19141 cggactatgc gccgccgctg gcgaatgtcg ccacgccaaa cgcggtagtc actaaagggc
    19201 atcgctttga tatttacgcc ggaacgccgg tgtaaaagta aagcggggta gcgataacgc
    19261 taccccgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat gcgtttcagc
    19321 cacggcacca tcaatagcat cactactgca acgcccagcg taaccaggcc aatcttacca
    19381 aacacgttgg tatagacggg caacgtctca agtggatcgg taatgttgtc tggtactgcg
    19441 gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag gaaccacatc
    19501 ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc caggccaagg
    19561 gcgctaataa acagttcacc taagctctgg aataagtaca ccagcacgat aaaccatggc
    19621 gatgtcagcc cctgggcatc cgcaaaccac attccagccg ctgcagccgt caaaaagccc
    19681 agtgagcaca taaacatgcc gagagtaaac ttcatcggca tcgagagatc tttgcctttg
    19741 ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac ccagaacggg
    19801 ttaagcgcct ggaagctgac cgggttgatg gaaaagccga gaatttcatg atgcacgttg
    19861 ttgatggcaa agaagttcag cgacgttggc atctgggcgt agagaatgta aaacaccacc
    19921 gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt atccagcttg
    19981 aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag caccagattg
    20041 gcgacttcta cgttgtgcat cagccaggcg cagacgaaga tcatcaccac gctgccaagt
    20101 aatacgtaca acagtttact gaagctcatc ggccggaagt cgggttcaga accaatgtct
    20161 ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc tgcaccgcac
    20221 aggttgtagg tgactgaata gccgaattta tcagcgatca caggggccag cgataacgct
    20281 atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc aagccgcgga
    20341 tctttcggcg ggtagcactt cgaaagcaag ctggctgggt tagctttaaa caggccgtta
    20401 ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag cgacatgccg
    20461 gtcatgaagt agccaatcgc cagcacaagt gctccgagaa caatggtgcg tttggttccc
    20521 agcaggtggt cgccaacata gccaccaatg gaaatgaggc catagaccag cgcagcaaaa
    20581 gcaccaaaag tgacaaaagc ctgctcttgc gagaacccaa gctgtttaac gaagaaaacc
    20641 gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac aaaaaagatc
    20701 atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt catatttatt
    20761 aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc gaaaaagttt
    20821 acatctgtca gagattctta atattgatgc ggcgaataga ttaatacact attacaacag
    20881 aaaataacca gctgatcaca ctaatgatag aaaatatgat ggtgttaaat gttgtattgg
    20941 caggggtttt gattgagtac gcagaccgca ggccagataa ggcgtttacg ctgcatcagg
    21001 taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg
    21061 gagggaagat tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt
    21121 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
    21181 aaccaccaga tccatatcgt atttcttgat tgcatcgacc agtacctggc ccagatcgcc
    21241 gctgccgctc agggtttcag tgattgggta gcctgcattg gttgaaagct cggtcagcgc
    21301 atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat cgataagccc
    21361 ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg
    21421 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga ggtcgaccgc
    21481 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
    21541 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
    21601 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
    21661 cgtcaatccg ccttgcttac tcgttagata aacaaatttt cgacatgaat atattgatag
    21721 tggttaacct tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg
    21781 atgttggtca ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat
    21841 cgggagcggt agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata
    21901 agcaccgtcg cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat
    21961 ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa
    22021 tatgttgatg gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc
    22081 gtttggtata tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc
    22141 tgtgagcggg tgcgtcggca gtttattctg accagcgcat tgtgtggtct ggtggtggtc
    22201 agcctgattg cattgatgat ttggcattaa gcaattcagc aataatgacg cataaaaaaa
    22261 gcgggccagt aaactgaccc gctcgttttg gtgcgtacaa ttacaggaac tgcaaggaga
    22321 gccagtacag cccgccggaa agcagtaccg cagccggaag ggtaaacacc caggccatca
    22381 gaatactggt tacggtttta cgctgtaagc cgccgccatc taccaccatc gtccccgcga
    22441 cagaagagga gagtacgtga gtagtggaaa ccggcatccc ggtataactt gccaggccga
    22501 tagacactgc cgccgtcatc tgggcagaca tcccctgagc gtaggtcatg cctttcttac
    22561 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
    22621 acgccagcgc gaccgccatg atgatccaca ccggcgcata ctcgatggtg ctaagcatgt
    22681 cggacttcag tttcttcaac aggcgctgat cgtcagcact cacaccaggc atcttcacca
    22741 ctttgtcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    22801 gcgacagctt gtcgtagctt tccacatcgg tagtcaacat gcctttcagg cggttgagcg
    22861 cgttaatggt attcgacgga tggcaatgga actccgcggg ttgcgttgcg ccagcttccg
    22921 gagccggtac taactgatca gcaccggtcg cttgtttcag cagcgcagga tgctgctcaa
    22981 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    23041 tcatgttcac cacgaagcct gctggtgcga caccaatcaa taccaacata accagaccaa
    23101 tgcctttctg accatcgttc gcgccatgcg aaaacgccac gccgatagcg gaaaggatca
    23161 gcgcaatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    23221 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    23281 tcagaccgcc agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac
    23341 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    23401 ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa
    23461 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    23521 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    23581 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaataccgcc gccataacca
    23641 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    23701 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    23761 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    23821 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    23881 tttttttgat ttgcctcctg tatggattac attcaaaaaa taattatctt atataattca
    23941 ggcaaatact tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat
    24001 tcacgctcgt tttttcttag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg
    24061 cgctggtgcg gcaggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    24121 gctgatcgat aatggtaaaa aaccagggcg caaaatcctc atgtctggcg gtgggcgctg
    24181 caactttacc aacctttatg tcgaaccggg cgcttatctg agccagaatc cgcatttttg
    24241 taagtctgca ctcgcgcgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    24301 catcgcctgg cacgagaaaa cgttagggca gctcttctgc gatgactccg cgcagcagat
    24361 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgtg gccttcagat tgcgtagcga
    24421 agtgctgagc gtggcgaagg atgatacagg cttcacgctt gaactgaacg gcatgactgt
    24481 cggttgcgaa aagctggtca ttgcgaccgg tggactgtca atgccggggc tgggcgcatc
    24541 gccgtttggt tataagattg ccgaacaatt cggcctcaac gtgctgccga cccgtgcggg
    24601 tctggtgcca ttcactctgc ataaaccgtt gcttgaagag ttacaggtgc tggcgggcgt
    24661 ggcggtgcct tccgtgatta ccgctgaaaa cggtaccgtt ttccgtgaga acttactctt
    24721 cacccaccgc ggcttatctg gaccggcggt gttgcagatt tccagctact ggcaaccggg
    24781 tgaatttgtc agcatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    24841 gcgtaatgca catccgaatc aaagcctgaa aaacacgctg gcggttcatc tgccgaagcg
    24901 gttggttgaa cggttacaac aactcgggca aatcccggat gtctcgctaa aacagctcaa
    24961 cgtgcgtgac caacagacac tgattagcac attgactgac tggcgcgtac aacccaacgg
    25021 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactctc
    25081 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga
    25141 cgtcaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg catgggcttg
    25201 tgcgcaggat ttggtggcag aataagaggc aaaatgcgct ctcgatacca gagggattaa
    25261 cctgaataaa accagataag gcgtccaacg ccttatctga agcctttttt attatttttt
    25321 cgctgaaccc gcttctaata tagaaacgat ggaaaacagt gtaaaacaaa ctgcacctaa
    25381 gccaaccaga actcgcgaag gaataaagta agccatatcg gtttgcgcaa tttcagccag
    25441 gaacgcagca aggaacagac aaaacagaca ggtaaatacc ggaatcatgg gtattctgtt
    25501 ggctaacgaa caggtgcgtc gccataccag ggctaacaac cacactttgg agaaaatact
    25561 gtagcagatc atccccaggc aaatgagaat aatgccgggg gccaggcggg cgcttgcatc
    25621 tgaactgatt aatacgtaga taccaaaaat aatggtaagt gagccaagca aaataaccca
    25681 atagcaccac agccaatgtt ctttcactga gaacgtattc cgcgtttgat ggacaatagt
    25741 ggcgacaagg ccaataagac aagcgcaaat tgccgttaac cccattagta cgtgacctgc
    25801 aacataatgt ggtgtgatat ctgcactacg taacagtatg actgaccaaa tgaaaccaaa
    25861 gagagtaagc agaacgggaa cggcaattaa gcaattaccg attgttgaag aataggcctg
    25921 caatggcgta ccgtcactct tactgcccga ggcgtttttg gggatcaaca gaaaatgtcc
    25981 ggaagatgcc gctacggttg atacacaagc ggcaatcatg ccaacgccga aaataacatg
    26041 accggcgaca aattcatctg ccatcacatt attgcttgcc aacagtgacc atccccaaat
    26101 catagttgta gcagaaccgg cataaccaat aacaggaaat aacctgttgt agaatttatt
    26161 catgccgtga gttaactgag atataataat aaatgcggta gtgaacaatg ccagacatat
    26221 cgcagaaaga gaaatcagaa catgccctgc aacgaaatat tcactttgcc cataaccggt
    26281 taatacaaac tcacctaatg cgatgcatat tatacccatc agtaaaggaa ttaatttaaa
    26341 caaccatccg atatatatat tcattttaaa ccccatatat attttctgct acacttcgaa
    26401 aaacatatga ttcgtctttt cagttagtta aatatgtcta taagatgcca tcaattaaaa
    26461 aaagtaactt cactataatc ggatttagcg ttatggggat caacttttag cggtgagtt
//
LOCUS       DAZKHK010000075        26466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKHK010000075 DAZKHK010000000
VERSION     DAZKHK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.075"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            298..405
                     /locus_tag="ACHOS5_003033"
                     /pseudo
     CDS             298..405
                     /locus_tag="ACHOS5_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(649..984)
                     /locus_tag="ACHOS5_003034"
                     /pseudo
     CDS             complement(649..984)
                     /locus_tag="ACHOS5_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            1034..1609
                     /locus_tag="ACHOS5_003035"
                     /pseudo
     CDS             1034..1609
                     /locus_tag="ACHOS5_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1999..3321)
                     /gene="hipA"
                     /locus_tag="ACHOS5_003036"
     CDS             complement(1999..3321)
                     /gene="hipA"
                     /locus_tag="ACHOS5_003036"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP9777811.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVKRFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     QIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            complement(3321..3587)
                     /gene="hipB"
                     /locus_tag="ACHOS5_003037"
     CDS             complement(3321..3587)
                     /gene="hipB"
                     /locus_tag="ACHOS5_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP9777812.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEAAEQQDLEW"
     gene            3895..4653
                     /gene="tam"
                     /locus_tag="ACHOS5_003038"
     CDS             3895..4653
                     /gene="tam"
                     /locus_tag="ACHOS5_003038"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP9777813.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQISLAFPRLFIVARRTE"
     gene            complement(4657..5571)
                     /gene="yneE"
                     /locus_tag="ACHOS5_003039"
     CDS             complement(4657..5571)
                     /gene="yneE"
                     /locus_tag="ACHOS5_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP9777814.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(5768..7219)
                     /gene="uxaB"
                     /locus_tag="ACHOS5_003040"
     CDS             complement(5768..7219)
                     /gene="uxaB"
                     /locus_tag="ACHOS5_003040"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9777815.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEYTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(7446..8866)
                     /locus_tag="ACHOS5_003041"
                     /pseudo
     CDS             complement(7446..8866)
                     /locus_tag="ACHOS5_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(9005..9364)
                     /gene="yneG"
                     /locus_tag="ACHOS5_003042"
     CDS             complement(9005..9364)
                     /gene="yneG"
                     /locus_tag="ACHOS5_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP9777816.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(9364..10290)
                     /gene="glsB"
                     /locus_tag="ACHOS5_003043"
     CDS             complement(9364..10290)
                     /gene="glsB"
                     /locus_tag="ACHOS5_003043"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP9777817.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(10354..11742)
                     /gene="sad"
                     /locus_tag="ACHOS5_003044"
     CDS             complement(10354..11742)
                     /gene="sad"
                     /locus_tag="ACHOS5_003044"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP9777818.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWASVDDIENALQLAAAGFRD
                     WREANIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRN"
     gene            11843..12724
                     /gene="yneJ"
                     /locus_tag="ACHOS5_003045"
     CDS             11843..12724
                     /gene="yneJ"
                     /locus_tag="ACHOS5_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9777819.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            12802..12921
                     /gene="yneK"
                     /locus_tag="ACHOS5_003046"
     CDS             12802..12921
                     /gene="yneK"
                     /locus_tag="ACHOS5_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFP9777820.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            13063..14253
                     /gene="ydeA"
                     /locus_tag="ACHOS5_003047"
     CDS             13063..14253
                     /gene="ydeA"
                     /locus_tag="ACHOS5_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP9777821.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(14278..14943)
                     /gene="marC"
                     /locus_tag="ACHOS5_003048"
     CDS             complement(14278..14943)
                     /gene="marC"
                     /locus_tag="ACHOS5_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP9777822.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            15155..15589
                     /gene="marR"
                     /locus_tag="ACHOS5_003049"
     CDS             15155..15589
                     /gene="marR"
                     /locus_tag="ACHOS5_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP9777823.1"
                     /translation="MKNTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            15610..15993
                     /gene="marA"
                     /locus_tag="ACHOS5_003050"
     CDS             15610..15993
                     /gene="marA"
                     /locus_tag="ACHOS5_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP9777824.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            16025..16243
                     /gene="marB"
                     /locus_tag="ACHOS5_003051"
     CDS             16025..16243
                     /gene="marB"
                     /locus_tag="ACHOS5_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP9777825.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(16300..17739)
                     /locus_tag="ACHOS5_003052"
     CDS             complement(16300..17739)
                     /locus_tag="ACHOS5_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9777826.1"
                     /translation="MSGFKEDFLWGGAAAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAGMGFKCFRTSIAWTRIFPQGDEQES
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIHFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDLNEREQIMYQAVHYEL
                     VASALAVQTGKLINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPDDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFSSHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(17763..19436)
                     /locus_tag="ACHOS5_003053"
     CDS             complement(17763..19436)
                     /locus_tag="ACHOS5_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9777827.1"
                     /translation="MNIKTLNVSLLSISIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKFRLSTLQKSITENKYQSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQIAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGRTANAVVTYDGNVGEQYNDDWFG
                     DSANENIMQFSDIYLTTRGFLPFAREADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(19491..19802)
                     /locus_tag="ACHOS5_003054"
     CDS             complement(19491..19802)
                     /locus_tag="ACHOS5_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9777828.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATKLMASS
                     ESACLQAHKIQTALISQDEGCGKIEVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(19830..21152)
                     /locus_tag="ACHOS5_003055"
     CDS             complement(19830..21152)
                     /locus_tag="ACHOS5_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9777829.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNVAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(21267..21578)
                     /locus_tag="ACHOS5_003056"
     CDS             complement(21267..21578)
                     /locus_tag="ACHOS5_003056"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9777830.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHATAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKAEIEVVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            21777..22475
                     /locus_tag="ACHOS5_003057"
     CDS             21777..22475
                     /locus_tag="ACHOS5_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9777831.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(22520..23419)
                     /gene="eamA"
                     /locus_tag="ACHOS5_003058"
     CDS             complement(22520..23419)
                     /gene="eamA"
                     /locus_tag="ACHOS5_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP9777832.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            23614..24801
                     /gene="ydeE"
                     /locus_tag="ACHOS5_003059"
     CDS             23614..24801
                     /gene="ydeE"
                     /locus_tag="ACHOS5_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP9777833.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            24928..25023
                     /gene="mgtS"
                     /locus_tag="ACHOS5_003060"
     CDS             24928..25023
                     /gene="mgtS"
                     /locus_tag="ACHOS5_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP9777834.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            25023..25127
                     /gene="mgtT"
                     /locus_tag="ACHOS5_003061"
     CDS             25023..25127
                     /gene="mgtT"
                     /locus_tag="ACHOS5_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP9777835.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(25242..26132)
                     /gene="dgcZ"
                     /locus_tag="ACHOS5_003062"
     CDS             complement(25242..26132)
                     /gene="dgcZ"
                     /locus_tag="ACHOS5_003062"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP9777836.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTVRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTCDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(26386..>26466)
                     /locus_tag="ACHOS5_003063"
     CDS             complement(26386..>26466)
                     /locus_tag="ACHOS5_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
                     /protein_id="HFP9777837.1"
                     /translation="DQMINISGSLDKKSAPAVVRVTQLQK"
BASE COUNT         7118 a         6497 c         5885 g         6966 t
ORIGIN      
        1 attacatttc acgaagaatg ataatatctt taaattacaa ggacacctat tcacaacaag
       61 tttattgctg cacttgttgt agagagtatt tgtaaaaaca ttttatgtcg ctaaataaca
      121 tgacgatttc attatgcatg ttatttataa acagcgacta aacagaatat tattgcgaac
      181 aagggaagtg tcaggaaagg tcaagaggta ctaatagttt atatggctat aaacattagc
      241 tatggttaat atttgcctgg tgaaactaac accaaattgc tgtaactctc tttgaatcat
      301 aaactgaagg atgatgcagc acagttcctc gtggactatc ctggctacta catgaccaaa
      361 cgcggatatc atcctcgagc agtcaactct ggccatcttt ttactcagct atgaggacct
      421 gatcgtcgac tttgctatct gcatgatgaa tctcctcagc agcagatgca gtatttaccg
      481 aaatgaattc catgaataat gcagtaatta gtagtgcttt catatctgat acctgtttta
      541 aacgatatat ttgaaagttg acgaatatcg gaaatagctt cctccttctt gatctgtaaa
      601 tatacagttc taagctgtca taagactgac tgtcatatga caacgttgtt agatttttga
      661 atttttcgaa tacagattca ggtcgttaat aacgcactca ggttcttcag taaggggcat
      721 attgtctata tcgaaaaccc catcattttt ttgtggatta tcattccgca gctctgcaaa
      781 cagaggagtg agatcctgct gtgcttctcc tcgctctgcg accagttcga acgttttatt
      841 ttttgccgca tcgttgctca gagctgttac aagaacctgg gcgatttgct cccgggagat
      901 aacaccatct tccggcgttc ccgcatggcg tctgtccccc tgaagcatga cgattctgtg
      961 ctcatcatca ttgttgtaat caaacccctt catgaatgcg ctttgaaggg aatgcttcta
     1021 caaggaaatc tcaatgatta aagaaaactt caacgaactg caaatctttc ttgtggtggc
     1081 aagagagcga agttttacca aagcagcaag caaactcggc gtctctcagt ctgcactcag
     1141 ccatgcaatg aaggcactgg aggaaaggtt aaatatccgt ctcttaaccc gaactacccg
     1201 tagcgtgacc cccacggaag caggggagag aataattgcc tgtcttgagc cacgtataga
     1261 tgagcttgag cgcgagctgg aatcacttat ccaactgaat ggcgcgccat ccgggaatat
     1321 tcgtttatca gccggggaac atgctgcacg cagtctggtc tggccgaagt taaaatcgtt
     1381 cctcagagaa tatcccgaaa tcaatgtcga actggtggtg gataacggtt ttgtcgatat
     1441 tgttgacggt cgttttgatg ccgggatccg gctgggtgaa agtgtggata aggatatgat
     1501 tgcagtgcga attgggccgg atatgcggat ggccatggtg ggatcgccgg cgtattttgc
     1561 tgcaaaccct gcgcctgaaa cgcctcacga actacaaaat catcgctgca tcaataacgt
     1621 aacgtggata aacctgcgaa ttatagccag ggctacgagt actactggaa aacccacgca
     1681 cacccaaaca tattcagaag cgtgagggat atagtgccat caaagcgctg gctgctgtaa
     1741 acaacgcaat tatccaactc atggtctatg gtgtgccatc actgaacaga ggttgaattt
     1801 ttctaccgaa acgggaggga agtcagaaat cacaacgtat tgatttatct gtgtgtggat
     1861 gtgaatggtg aagtaaagga ccttcacagg taatttaaca taatatacat tatgcgcacc
     1921 aacataaacc aagggagaat ccagtcgttg gcggttcatg attgttctgc tcattaacaa
     1981 cgaccaaaac cccatatctt acttaatacc gtattctcgg cttaaccgac cgtgcaacct
     2041 caacacattg gtttcaactg ccgtcaccac gttctccgga aaatctgtcg gtaatgaagt
     2101 cttcacgtta tccagtgctg ccggaatcat tctggcaaag tcactcagga tttcatgcat
     2161 ctgcacttcc gggaatttca gcacctttgc tgtcgccaga aaatgtcgcg gataaatttt
     2221 atcgattgcc gtttttttgc ctttggatgc gttaagcccc attgccagtt tgagatcgct
     2281 gatgtgtatt cccgtaccgc caaggaccgg aaatgccgaa atgatgtcat aaaatggtgt
     2341 gagccgataa ctgccgccag cctgaataaa tacggagaag ttttttgcat ggccatctgt
     2401 tgcgccaatc aaccactgga agacctgaaa cttcataaaa tcatagcgat ctttcagcgc
     2461 ctcgctggac cccatcaaaa aagccataat ttgcgcgata ccggggcctc catctgattc
     2521 atatttcacc gatgaaggta aaccgaatgt ctggcacata tcctcctgtg gcaagcgaag
     2581 taaaaccgtt cgctcagtat tccaacgcct gtcaaaacgt ttgaccgcta acgcgcgcac
     2641 ccttcccgct ttaatgattt ctgcgtccgg aacattcaac ccgagttctt tcgccagcag
     2701 caaacagtaa tactcattat caacgctttg gctaagatcg agcgtcgcat tgggctgtct
     2761 gatttcgcca atcggtaatt tgataatgtg cgtcgttggc gttattccct tcggaatgca
     2821 ccagtcattg cctattctga gcagcgcagt cttctcctgt gcgccagcaa ccgagatgcg
     2881 aaagtcattt tcttctctaa tcatgcctaa cgggatatct gctttataag ccgttaatac
     2941 ttcttcaagt ctggcttcag taagcttttc ccatgccatt atcggacacg ttacggtttc
     3001 atcttcgggg attaacgtca cggcaccaac gctatctcgc cctatttctg acaataaatc
     3061 aaacggttgt ctggatttag catgataacg tttaacgatc cggtcacgta caatcgggct
     3121 atcgggtaac aggttatcga agaagttaaa tacggcatca gaggtgatat tccccctctg
     3181 caatggcagc gaaagtgaca acggtctggc ataacggctg gctaaccact caggtgcata
     3241 cttaaaggtg tgcgcgccgt tggctaactt cgttaactcg cctacccgct ggttgttcat
     3301 ccaagtgact agtttaggca ttaccactcc aaatcttgct gttctgctgc ttctggcgag
     3361 gcatttttcg cgtcgcatag cgtcattgag agttcaagcg actgtaaaat cttaaaaaat
     3421 gtcgtgagcg aggtattgtc aggattgttt tcgaaattgg aaattgtcgc ctgcttaatg
     3481 ccgatttttt tcgccaactc gctctgcgtc cagccgtttt gctggcgaac cagtttcatt
     3541 gcattcgcca attgcgttgg gctatagatc ttctgaaagc tcatcatgtc atacgtccac
     3601 agcaaattta tccgcttaag gggatattat cagttttatc ctttagtgag gataaagtca
     3661 atctggaaac acatcttatc cgctctacgg gataaacgct gcgatatccg cgatcgcgga
     3721 taaaaaggag gatttaaaaa agcacatcct aatgcggtgg cttctttgac taccggaatc
     3781 tgtaatctta atgacctggc cgcgtaaaaa acgtctgttc aatggggtga tgccgtgagg
     3841 cgaatttatc agttttatct acaattgggg taacgcgctg acgggagtaa aaaaatgtct
     3901 gactggaacc cctctttata tctgcacttt gccgctgaac gatcgcgtcc ggcggtggag
     3961 ctgcttgcca gggtgccgct ggagaatatc gaatacattg ccgatcttgg ctgtggccca
     4021 ggtaacagca ccgcccttct acaccaacgt tggcctgctg ccaggataac gggcatcgac
     4081 tcatctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcct gtttgtggaa
     4141 gcagatatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctcg
     4201 ctgcaatggc tgcccgacca ctacgaattg tttcctcatc tggtttcgtt actcagtccg
     4261 ctgggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     4321 cgcgaagttg cctgggaaca aaactacccg gatcgcgggc gtgagccgct ggctggcgtt
     4381 catgcttact acgatattct aagtgaagcc ggatgtgagg tcgatatctg gcgaaccact
     4441 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     4501 cgtccgtggt tacaggatct gaccgagagc gaacagcagc attttcttac gcgctaccat
     4561 cagatgctgg aagagcaata tcccctccag gagaacggac agatatcgct ggcatttccg
     4621 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatggcgatc
     4681 gggaagaatt ttcgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     4741 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaagg gatcttccag
     4801 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     4861 cgtcatgtaa tgcaggtcca cgaccagcgc aaacggcagc atgatacaaa acagatacac
     4921 ggtgcgatgc aaaatcaggg tgtaggcaaa gggaattggc gtataggcaa tgcgctcgca
     4981 tcctgccagg accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     5041 tgaaagttgt ccattgcgtc gctgaaccgc caaccactcc cccattatta acaagatgcg
     5101 gttagctgga gagttagaag ccagtacacg ctgaagatct tcagtcttga gataatgagc
     5161 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     5221 ttgcagccgg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctcgcg
     5281 cagtaacgac cgcgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     5341 ggcgtaaccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     5401 gaacggtgcg agggtgaatt taatgcccaa atgcgtgtac cagggcagca tgaaaataac
     5461 agcgatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgaacc
     5521 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     5581 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacactactt
     5641 acaaattacc tacaagctta caacattaac aggtgctgca tgtggcacct gctaatgata
     5701 aaggcatata gaatgttgta actaatatag cgaaataaga aacccggtcg aaaccgggtt
     5761 cagagtatta gcacaacggg cgtacagctt cgcgcatccc tttttcgaga atcgcatcca
     5821 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
     5881 ctttttctgc caacacaatc gctaccagtt cctgggtccc gataacacgg tcacgatact
     5941 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
     6001 ctccattacg ctcaccgcga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
     6061 ccggaagtgt gccgtttgcc ttctgccctg ccagcagctg cggcaggatg cgtgtgcgga
     6121 acttggtcat accgttgagc gcgatagaca gcagttgatg cttgatgtac gggttacgga
     6181 aacgtccggt gactgcactg gcgaaagact ccagctcatc gcgtggcaaa tccagtaccg
     6241 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
     6301 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
     6361 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaacg atcagcacgt
     6421 tgagcggata tttgtccaga cgaagttcag tcgctaagga tttcggcccc tgaatcacaa
     6481 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg gtagcccagt tcttcttcca
     6541 gtttagccac ttcatcacgt ggataaccgg taacgatacg gtccaccagc gtagaacaga
     6601 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     6661 agcgcagcac cagttcacgc aacgcgtcgc cattatagtc aatcaactca cacgggatga
     6721 tgatccaacc tttatccagc gcaccgttga aatggctaaa acgttcgaac agcagacggg
     6781 tcagttttgc cggataactt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     6841 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgagccagtt
     6901 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcaggc
     6961 gcgcgtcgct gaccgcttca cccttctcat tcaggccacg gataatggtg gtataaagac
     7021 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     7081 cagaattcag atcggtgtac tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     7141 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     7201 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt
     7261 ggtacgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     7321 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaacagg
     7381 ccttttcttg gatcataaag tggtagaaca cattgcattt aattcgctct gagtaaataa
     7441 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     7501 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaagtc atcggtcagc
     7561 gttcggtacg atgcgcaacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     7621 tgttgccagg tgaacggttg aagttctacg cctttacgga ttttttccgc cattagcagg
     7681 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc cattcgtgcc
     7741 accagcccct tatcaccgac aatcttctga atatgctggg caaacacgct taacacttta
     7801 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     7861 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg
     7921 gaccgtgagt aaacctgggt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     7981 agcgaattga tcgctgccac gctaaaggaa accataattg ggcatatcgc catcgtggca
     8041 ataccgagac gtgcggagaa catttgcgga atggagaacg gcgaaccgac cgagatatca
     8101 atcaccgaat ttgccaccag tacgacttcc acagcaccgg tgacaaaggt caacagacat
     8161 gttacctgcg gcgtatagcg cactgcacac cagattaatg cagggagcgg aaacgccaga
     8221 ctccccgctc cgccaatgac taccgaggct ataaccgaca caataagcgc gatagcaggc
     8281 atcatctgct ctgctttaaa gcgcggcagc actccaggaa ttgccaacgt cagcatacaa
     8341 ggcacgatca acacgcccgt tgagaattgc tcactaaacc agtcggcaag caaaggccag
     8401 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     8461 gcaagcagac agtaattaaa tagccgtaag gcacttactg gctcatactt attttttccc
     8521 agacgcttat cccgtgcgac cagtataagg cgacggtaac aataaacatc atattggaga
     8581 aattgatagc cagtgaaacc agcccccatt cggtggttat ggcatcataa accagcatcg
     8641 ccacataact gatcgcatag taatgcagtc gattaagcca gacatagcgg gcaaacaccc
     8701 ccgccattac gccattcagc ggccagaaaa gagaaagttc gtgaaccagt cgtaactctg
     8761 caccaataaa atagaacagc gtggttagca caaaaatagc gatgctatta cgtagcagat
     8821 gaccttcctg gaacaaacgg aaagttgaaa tgggctgtac atgcattaat tatctgtcca
     8881 tacaattgcg ggtggcaatt atctcaacgc aataagtaaa ttcttagaaa ggatagcatg
     8941 ttcgctgaca acggcggcaa ctaaagatac ctaatgaatg ccattttatc gatttccgta
     9001 attatcatgg ctgattcatc tgtataacca gccagtggta gattaccgtc acaatataac
     9061 gttgttgctc ctcacttaac gcttccccct gaggaatgtt atgccactta gccagacaac
     9121 cgcgacaaca ggtcgcggtg gcgtgttgag cgataaaaac cggatgcccg cgcatggggg
     9181 tttgcttacc gtcgttagcc ggtaatgccg gagcaagacg tttagcgaca aaatccgccg
     9241 catgttgttc aatgactggc gcgcctttct ccagacaata ctgacgctct ttcacaccca
     9301 aacgaaagcg agagcgaaat tttgaacggg ataaacgcgc gaagagtgga tcgagagact
     9361 gcattaataa actgaacgcc ctaattgttt cgtcaattgt tcaaggactg caatgcccgc
     9421 aagcgagtta ccggcatcat ccagttccgg actccagaca gcgatggcca tttcatgcgg
     9481 aacaatcgcc acaataccgc caccaacgcc agattttgcc ggtagtccca cccgccaggc
     9541 aaactccccc gcgttctggt acataccgct ggtcgccatc agcgcgttaa tttgccgcgc
     9601 ctgcattggc gtcaccacag gttcatcaat atgaatagct ttcccctgat tcgccagaaa
     9661 gacaaacgtc cgggccagct ctacacagct cattttcaga gcgcagtaat gaaagtagtt
     9721 ttgcagaacg gttgtcacgt catgatggaa attgccaaac gacttcatca gccaggcgat
     9781 agccgcattt cgcgcggaat gttcaaattc ggaacgcgct accaccgtat cgtaggaaat
     9841 atcagacaca ccacttaagc cgcgcacgac ttccagcata cgttgccgag gagcgcttaa
     9901 tcgcccttgc aacatatcgc agaccaccag cgcaccggca ttaataaacg gattacgcgg
     9961 aataccctgc tccatttcca gttgcactaa agaattgaac ggtgatccag acggatcttt
    10021 gccgacgcgt tgccagattt cctcttcgga gtaatgacgc atggcgacaa cgagactcag
    10081 cactttggaa atagactgaa tggaaaaacg ttcttgcgcg tctccggcct gaaaaagctg
    10141 tccgtcaacg gtacagatag caatccccaa tcgggaaccg tctaccgtag ccagcgccgg
    10201 aatataatcc gcgactttac cctgaccaat gagcggccgc acttgccgca agatgttttc
    10261 taaaattgca ttatccatgg cgactgccac tttctactcc tggaccgcag gtctgaaaag
    10321 acctgcgagt atatcagagc tgaatatgta gcgtcagttc cggtctttcc acaccgtctg
    10381 gatattacag aattcgtgta agccaaaatg ggaaagctca cgcccaaagc cactcttctt
    10441 cacgccacca aaggcgactc gcgcatcgct ggcacaataa ccattgataa acaccccacc
    10501 gcattccaga cgtgccgcca tctgtctggc ctgtgtttcg tcggtggtga aaatggtcgc
    10561 tgaaaggccg aactcactat cattagccag ttccagcgca tgttctgcat ctttcgcaat
    10621 ggtgattgcc gcaacggggc caaacatttc ttcccgaaac gcggtcattt ctggggtaac
    10681 attcgccaga accgttggcg gatagtagtt acctgcccca gccatctttt ccccgcccag
    10741 taacaaacgc gcaccctgcg ccagtgtttt ttccacctga tgatgcagct catcacgtaa
    10801 atcaaaacga gccattggtc cgagagcgtt ctcttcatca cggggatcgc ccattttcaa
    10861 ggctgccgca gctgccacaa aacgttcggt aaatgccgaa gcaattccct cttcgatgat
    10921 aaagcgtttt gccgctgcac aaacctgtcc ggtattctga taacgtccgg ctaccgccgc
    10981 tttcaccgcc agttccagat cggcatcgtt aagcacaata aacggatccg aaccccccag
    11041 ttccagtacg cattttttca gtgccgctcc agcctgtgcg ccaatagccg ctccagcacg
    11101 aacacttccg gtcactgtga cagcagcaat gcgcgagtct ttaatcatca gactgacacc
    11161 gtcgttgtcg gcattcagcc agccatatac gccttgtggg atacccgcat ctttaaacac
    11221 ctgggcaatg agctgtgcac agcccatcac attcggcgca tgtttaagta agtagccgtt
    11281 accagcaaga atgatgggaa cagcgccacg catcacctgc cataacggaa aattccacgg
    11341 cataatcgcc agaatcgtcc ccaacggtcg atactcaata accgcctgct gattttccac
    11401 cagcgtaggt tctgccttca gcattgctgg accatgttct gcataccagt cacacaaatt
    11461 cgccgatttc gccacttcag cgcgcgcctg gttgattggt ttgcccattt cacgggtgat
    11521 catttgcgcc atttcttcgc tacgggcgcg cagagcctta ccgatatcac gcagtttttc
    11581 agcacgataa tctatatttg cctcgcgcca gtcgcgaaag cctgctgctg ccagctgaag
    11641 cgcgttttcg atatcgtcaa cactagccca cggcagcaca gaaagttgtt caccggtggc
    11701 aggatttatc gaaattgcat gagttgccgg agtaatggtc atcggggtat ctcctttatg
    11761 agtcatggta tgaagatacg cagatttact cttgctttaa aatgaataat attaagccac
    11821 ttattcacga atcgagaatg ctatggatct gacccaactg gagatgttca acgcggttgc
    11881 cgaagctggc agcattaccc aggctgcagc aaaagtgcat cgcgtgccgt cgaatctcac
    11941 tacccgtcta cgccagctgg aaacagaact gggggttgat ctgtttattc gcgagaatca
    12001 gcgtttacgt ctctcgcctg ccgggcataa ctttttacgc tatagccaac aaattctcac
    12061 gttagtggat gaagcgcgga gcgttgtcgc tggcgatgag ccgcaaggtt tattttctct
    12121 gggatcgctg gaaagcaccg ctgcagtgcg cattccggcc acgctggcgg aatttaaccg
    12181 tcgttatccc aaaattcagt tttcactttc caccggccct tccggcacta tgctggaagg
    12241 tgtgctggag ggaaaactga atgcggcgtt tattgatgga cccattaacc atactgccat
    12301 cgacgggata ccggtatacc gcgaggaact aatgatcgtc acgccacaag gacatgcgcc
    12361 agtaacccgt gccagtcagg tgaatggcag taacatttat gccttccgcg ccaattgttc
    12421 gtatcgtcgc cacttcgaaa gctggtttca tgctgacggt gccgctccgg gaactattca
    12481 cgagatggag tcttatcacg ggatgttggc ctgtgtggtc gcaggagcag gcattgcgct
    12541 tattccacgc tctatgctgg aaagtatgcc ggggcatcac caggttgaag cgtggccgtt
    12601 agctgagcaa tggcgttggt taacaacctg gctggtctgg cgtcgtggtg cgaaaacgcg
    12661 tccgcttgag gcatttattc aactgctgga tgcgcctgac tcggcaaaac agggttacca
    12721 atgagctatt tttgatagtt catgcggata atatgctcta tatagtgatg ttccgatgac
    12781 ttatgactat atggggcaaa tatggttacg ccagtaagca tcagcaatta catatctctt
    12841 cctgatgatt ttcccgttcg taatattgca cctcaagtaa aagaagaaca ggcccccttc
    12901 ctgatcgtca ttaaaatgtg atgttaacaa ctctctgatg cgtgacagaa caaccgtcag
    12961 agagatggaa atgcgatcca gatcacaaat gcattgtatt cacatcatta accgttttaa
    13021 gatcatttca tcactttttc gcaactcacc cgataatctg ttatgacaac aaacactgtt
    13081 tcccgcaaag tagcgtggct acgggtcgtt acgctggcag tcgccgcctt tatcttcaat
    13141 accaccgaat ttgtccctgt tggcctgctc tctgacattg cgcatagttt tcacatgcaa
    13201 accgctcagg tcggcatcat gttgacaatt tatgcatggg tagtggcgct aatgtcattg
    13261 ccttttatgt taatgaccag ccaggttgaa cgccgcaaat tactgatctg cctgtttgtg
    13321 gtgtttattg ccagccacgt actgtcgttt ttatcgtgga gttttaccgt tctggtgatc
    13381 agtcgcattg gtgtggcttt tgcacatgcg attttctggt cgattacggc gtctctggcg
    13441 atccgtatgg ctccagccgg gaagcgagca caggcattga gtttaattgc caccggtaca
    13501 gcactggcaa tggtattagg tttacctctc gggcgcattg tggggcagta ttttggctgg
    13561 cgaatgacct tcttcgcgat tggtatcggg gcgcttatta cccttttgtg cctgattaag
    13621 ttacttccct tgctgcccag tgagcattcc ggttcattga aaagcctccc gctattgttc
    13681 cgccgcccgg cattgatgag catttatttg ttaactgtgg tggttgtcac cgcccattac
    13741 acggcataca gctatatcga gccttttgta caaaacattg cgggattcag cgccaacttt
    13801 gccacggcat tactgttatt actcggtggt gcgggcatta ttggcagcgt gattttcggt
    13861 aaactgggta atcagtatgc gtctgcgttg gtgagtacag cgattgcgct attgctggtg
    13921 tgcctggcac tgctgctacc tgcggcgaac agtgaaatac acctcggggt gctgagtatt
    13981 ttctggggga tcgcgatgat gatcatcggg cttggtatgc aggttaaagt gctggcgctg
    14041 gcaccagatg ccaccgacgt cgcgatggcg ctattctccg ggatatttaa tattggaatc
    14101 ggcgcgggtg cgttggtagg taatcaggtg agtctgcact ggtcaatgtc gatgattggt
    14161 tatgtgggcg cggtgcctgc ttttgctgcg ttaatatggt caatcatcat ttttcgccgc
    14221 tggccagtga cactcgaaga acagacgcaa tagttgaaag gcccattcgg gcctttttta
    14281 atgatacgtt ttaatgattt ccaggatgcc gttaataata aactgcacgc ccatacatac
    14341 cagcaggaat cccatcagac gggagatcgc ttcaataccc cccttgccca ccagccgcat
    14401 aattgcgcca gagctgcgta gacttcccca caaaataacc gccaccagga aaaagatcag
    14461 cggcggcgca accatcagta cccaatcagc gaaggttgaa ctctgacgca ctgtggaggc
    14521 cgagctaata atcatcgcaa tggttcccgg accggcggta cttggcattg ccagtggtac
    14581 aaaagcaata ttggcactgg gttcatcttc cagctcttcc gacttgcttt tcgcttccgg
    14641 tgaatcaatc gctttctgtt gcggaaagag catccgaaaa ccgataaacg cgacgattaa
    14701 gccgcctgca attcgcagac cgggaatcga aatgccaaat gtatccatca ccagttgccc
    14761 ggcgtaatac gccaccatca tgatggcaaa tacgtacacc gaggccatca acgactgacg
    14821 attacgttcg gcactgttca tgttgcctgc caggccaaga aataacgcga cagttgttaa
    14881 tgggttagct aacggcagta acaccaccag ccccaggcct attgctttaa acaaatctaa
    14941 cattggtggt tgttatcctg tgtatctggt ttatcagcga aaagtataag gggtaaacaa
    15001 ggataaagtg tcactcttta gctaaccttg catcgcattg accaaaactt gaaccgattt
    15061 agcaaaacgt ggcatcggtc aattcattca tttgacttat acttgcctgg gcaatattat
    15121 cccctgcaac taattacttg ccagggcaac taatgtgaaa aataccagcg atctgttcaa
    15181 tgaaattatt ccattgggtc gcttaatcca tatggttaat cagaagaaag atcgcctgct
    15241 taacgagtat ctgtctccgc tggatattac cgcggcacag tttaaggtgc tctgctctat
    15301 ccgctgcgcg gcgtgtatta ctccggttga actgaaaaag gtattgtcgg tcgacctggg
    15361 ggcgctgacc cgtatgctgg atcgcctggt ctgtaaaggc tgggtggaaa ggttgccgaa
    15421 cccgaatgac aagcgcggcg tactggtaaa acttaccacc agcggcgcgg caatatgtga
    15481 acaatgccat caattagttg gccaggacct gcatcaagaa ttaacaaaaa acctgacggc
    15541 ggacgaagtg gcaacacttg agcatttgct taagaaagtc ctgccgtaaa caaaaaaaga
    15601 ggtatgacga tgtccagacg caatactgac gctattacca ttcatagcat tttggactgg
    15661 atcgaggaca acctggaatc gccactgtca ctggagaaag tgtcagagcg ttcgggttac
    15721 tccaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt aggccaatac
    15781 atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa cgagccgata
    15841 ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg aaccttcaaa
    15901 aattactttg atgttccgcc acataaatac cggatgacca atatgcaggg tgaatcacgc
    15961 tttttacatc cattaaatca ttacaacaac tagttgataa cgtgacaacg tcactgaggc
    16021 aatcatgaaa ccacttttat ccgcaatagc aactgcgctt attctctttt ctgcgcaggg
    16081 cgttgcggaa caaaccacgc agccggttgt tacttcctgt ggcaatgtcg tggttgttcc
    16141 cacatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca gtgataagtc
    16201 ggatgcgctc ggcgtgccct attataatca acacgctatg tagttattgt caggctctga
    16261 tcacagagcc tgacatttcc gtaatatctt cctgaagtct catatatttt cgccattgtt
    16321 ggcgatgaga tcccgatacc agtaaaacga tgctttacgt atccgttcca gcgttccctt
    16381 cccttcgttg tctttatcga cataaatcat tccgtagcgt tttttcattt ctcctgttcc
    16441 ggcagaaacc aggtcaatgc aaccccacgg ggtgtagcca attaagtcaa caccatcttc
    16501 aactacggct tttttcattt cccgaatatg ggaggaaaag taatcaatgc gatagtgatc
    16561 gttcaccgtg ccgtcagctt gtctctggtc aaccgcacca aatccatttt cgacaataaa
    16621 cagcggcaac tggaaatgat cccagaacca gttgagtgaa taacgtagcc ctgccggatc
    16681 aatttgccat ccccattcgg atgtatcgat ataagggttg ctgaccaaat ctcgtggttc
    16741 aacataatcc agttgcggat tatctggcga aaattgcgtc gtaaaagaca tgtaatagct
    16801 aaagccgata aagtcgacac aacctctggc aagaatcgcg ttatcatctg gtgtgatatc
    16861 gaggttgaat cctttccttg caaagtaatt cagcatatgt tgcggataat atccacgagc
    16921 atgaacatca gtaaaccagt aacgacgatg catcgctttc gtggccatca tcatatcgtt
    16981 gggtgcacac gtcagaggat agatggggca catagcaatc atacagccga tattaaattc
    17041 aggattgatc agttttccag tctgtaccgc cagggcactg gcaactaact cataatgtac
    17101 cgcctggtac attatttgtt cgcgctcatt gagatcttcc tctggcgaat acaagatacc
    17161 ggaattagta aatggacaca ggctttcgct gaaattcacc tgattattga tttcgttaaa
    17221 cgtcatccag tactttactt tttctttata gcgcgtgaag accgttgatg cgaagtggat
    17281 aaaaaagtcg atcagtttac ggtttcgcca gccaccatat tttgtcacca gatgataagg
    17341 catctcaaaa tgcgaaagcg tcaccacagg ttccattccc tgcttcaggc attcatcgaa
    17401 gagatcatca taaaattgta aaccctcttc attcgactcc tgttcgtcac cttgcggaaa
    17461 gattcgtgtc caggcaatgg aagttcgaaa gcatttgaat cccatcccgg caaataactg
    17521 aatatctgtt ttatagcgat gataaaaatc aattgcttca tgattgggat aattaagtcc
    17581 gtcgataacg ccttctgtca cttcacgcgg caccccgtga gcgccagcag tcattacatc
    17641 agcgatactg atgccttttc ctccttcatt ccagccacct tccaattgat gtgcggctgc
    17701 cgcaccgccc cataaaaaat cttctttaaa tcctgacata cacaactcct taactaaata
    17761 gattaccacc agacttccgc ctggatgccg gccataaact cgtctttgct gttctcatta
    17821 aacttaaaat tcgataattc gttatccaga atattgatat acgtcccgta gaagcgaatt
    17881 tctggtcgtg aacctaaaat actttcaccc actttcaatg catgccagag tgtcgtttta
    17941 taagccgatt catttagcgt taccccctgc tgagttttgt tctgttgctt aaaccagcct
    18001 aattcaagcc ccgtctgatt ccatgtattc cagatccagg ccggtcttat tacggtgcga
    18061 atactgtcaa aatcactatg agcgccactt tcataactat aaacatcttc gccatgagaa
    18121 tagacaagcg cgttagccat aataatattg tcagtcagat acatctcgcc ctgagagatt
    18181 aaacgccagg cgatcccatt ggtatggtca ccatagtaat agcgaccatg cgccatcgta
    18241 ttactggcat ctgagaaact ggcaaaactg ctggcatagg aattattggc aacctgtaac
    18301 gtaaattcat taaacgtatc gcgttgcaag ttttgccgta aaacagaagt cagcatccag
    18361 ctatctttca tttcaaaata actgtcgtca ttttcattat cttgttgttg atccgtttta
    18421 ttaggtgcgg aatatttagc cattaatgac aacgtcgcat catcccataa cgggatattg
    18481 cgataacgaa catccacaga attggtattc atctgactgg tacgcgtaaa atcacgagag
    18541 taaacatcga catcatctcg gcttaaggac atatcaaaca gccctacacc aagcgcccag
    18601 ttttcaatcc ccacgcccgc ggcaacatcc gtggttaagg ttttccagtc cagcatttgg
    18661 atctcatatt gcgggagttt atgtttgccg acccagaagt ctgcctctcg tgcgaagggt
    18721 aaaaaacctc gcgttgtcag gtaaatatca ctgaactgca tgatattttc attggcactg
    18781 tcaccaaacc agtcatcgtt atactgctca cctacgtttc catcataggt aacgaccgca
    18841 tttgccgttc taccgtcctg attataaaca cgctgattta aggtcaggtc aaaccaacca
    18901 ctcatctcgt taccaaaacg tcccagagaa cccgctgcat aagtttgtgg tgagccgtga
    18961 ttaccggtgc cccagcctga gcggaagtac ccctgatagc tgaatccaat atcatccttt
    19021 atatatttac tgatatcttt cagggtcacg ctttcgatag tggttttatc gcctttactt
    19081 tcaataacgg caatttgctg cgatccattt atagtatgtg ctgcggcaga cgagttctgt
    19141 tcaccgtttt catttttgat gttatcagca acgggagatg tcgctgatat ttcggcaagc
    19201 gaggccgatt gatatttatt ttctgtgatg cttttttgta atgtcgaaag tcggaattta
    19261 tatactccca gttcattctg tgttgctttc agctcttgtt tattttgcga caattcattt
    19321 tcaagcaatt caaggcgctg ctctatggtt aattttgttg ccatcgcgtt caacggaaac
    19381 aatgctgtaa taatagaaat agacaaaagg ctgacgttaa gcgtctttat attcataact
    19441 tatccttaag agtgtagtaa tttcctccag atatcgcact ggagaaaaaa tcaggcatgg
    19501 agctcttttc ttaatgaaat aatttccctg gctaaatcct ggcataatat tgcattcatc
    19561 aaatgatcct gtgcatgtat cagaattaaa ttaacttcta ttttgccaca gccttcatcc
    19621 tgactaatta acgcggtctg aatcttatgc gcctgaagac aggcagattc agagctggcc
    19681 attagtttag ttgcgccctg ccagtctttt tgcccggcac aacgtatggc ttccatcgca
    19741 tcagaacgag cctgtcccgc gttgatcagt aactccataa caagttcttc atctgcaaac
    19801 atcacaatct ccttcctggg taaatggaat tatccgttag ttgtcatgtt attgactttg
    19861 ttggcggcga taacaaaagg taaatagatc attgtcgcca cacctaaaca taaaaaacca
    19921 accagtaatg ccagccagtt tcccccggtt ccgaggaaag caattaaagg tccgggcgta
    19981 gtccagggca cggcataagc aaccggtgga ataatttcca tcgaaacaaa gaagtaacca
    20041 atggcacagt taacaatggg aaccataata aacggcacca tcatgatggg gttaagtaca
    20101 ataggaaggc cgaacattat cggttcattg atattgaaaa taccggggat aaatgacatt
    20161 tttgccagat cacggtattc cgcacgctta gaagcgataa aaatagccaa caataacccc
    20221 aacgtcatac ctgaaccgcc gcagttggcg aatgcatcat taatagaagt ccaggtaatc
    20281 ggatatggcg cgccccaggt agtgccgtgt tgagcggccc aggagagatt ctccagatta
    20341 gcctcagaaa aaacggtttc tcgaattgcc gacgttgtat taggtccgtg gatccccagc
    20401 acccagagga aattacccac agcccccagg ataattaccg caaagatatt cgttcccata
    20461 tgtttgagcg gcgtctggat taatgtgtaa atgagatcat ttaatcctgc cggggatatc
    20521 aatgtcaggc aataattaag tgcggaaaag aacaccatga caaaaaatat tggcaataaa
    20581 actttaaatg aacgcgctac cgcaggaggt acagctgccg gcatcgttat ggtgattttg
    20641 gggtttcgag ctaagcgaca aaatatttca ctggtgatca atgcaacgat aacagcaaca
    20701 aacaacccct gcgcgccgag atattttgtc gtcaggaatg cctgcccatc tatcatctgg
    20761 tatggggtat aaacaataaa aaatgctcct attgccgtta atccacataa aagatcatct
    20821 tgctcatagc taatcgccac attcctggcg accaggaaag caatcataat tgacatgata
    20881 tttacagaac cattcattac cggagtaaaa atagcttgtg ctttttcaag gttggggaaa
    20941 atgctgttca gatgcagtaa tccggcaata aaaccgtcgg gcgataatat ggcaaagtta
    21001 attaatataa ttaatgagct tgccatgata attggaaacg taaaaacgaa tccatctctc
    21061 accgctgcaa catgtttttg tgagtttaac ttgatagcaa ctggaacaag aaaacgttcc
    21121 attccacgtt cgaatgatgc cattaatccc atttttttat acctgttgtt ctggatattt
    21181 atacatccca ataaagcatg tatgccatat ccattttatg atgtgtgaaa agttagttca
    21241 ggtaatagtt taataatatt agattcttac tcttgcagta gtttcatcgc aaattcaaga
    21301 actgcctgtc cgttcattgt gccatagtct ttcatctcga taacggctac aggcattttc
    21361 ccctgtgcaa cgacttcaat ctctgctttc tgaaagcgaa cttgcggacc aagtaaaaca
    21421 acgtcaactt cgttattttt aattttccct ttagcctctg caatcgccaa tgcggaaata
    21481 ttaacttcaa gtgaaatagc ggtagcatga tcaatcatac gtttaaccag catactggtt
    21541 gacatgcccg cagcgcaaac taacaatatc tttttcatat ttttccttac tggtatataa
    21601 cagactacat tgtgtaaata tcatatccca cagtagctac acgtcctggt acattgatca
    21661 cagtaataca gcaataaagt tgttcaacaa cagggtgata attaaacgac tcggccatac
    21721 gataaactaa tcaggtctca actccttgtt ttcaatagct cttaaaaggt gcaaaagtga
    21781 tttttcaaaa gattgcccgc ttacttaaat ccgagatcaa tggcaattca tggcatgttg
    21841 gtgatttgtt gccgtcagaa gcggaactcg ctgttcgtta taatgtttcg cgtaataccc
    21901 tgcgtaaggc attatccctg ctggaaggcg aaggaattat tcacagaaag catggttcag
    21961 gaacatacat tcaaaaaaag aattttgttg cacacattga tcacatgaac agtttcagtg
    22021 aaattgcaca taaaagcggc aaagaggcag gaagtcagat tatgaaattt gaagtgcagg
    22081 atgcttctcc tactattgcg acagagctga atttagtgac tggtgagcag gtttattaca
    22141 taaagagact gcgattcatt gaggataatg cagcacaatt agaagaaacg tggatgtcag
    22201 tggcacgttt tcctgattta accgtatcgc atatgcaaaa atccaaattt tcgtatattg
    22261 agaacgaatg cgggatcaaa atcattggca cctttgaaac tttctccccg acttttccta
    22321 ccccagaaat cgccagtatt ttacggatca gcccacggga tcccatactt aaaattcaga
    22381 cccaggctgt ggatagtaac tctattccgc tggattattc gttactttac agcaatattt
    22441 tcgagttcca ggtaaagtac tttttcccgc gataaaagca gacgcacaga gtgctggcat
    22501 cacattctgt gcgtatggtt tagcctctga cctttaccgc tttacgccac cgcaagccaa
    22561 atacattgat atacagcccg gtcataatga gcaccgcacc gaaaaattgc agacccgtta
    22621 agcgttcatc caacaatagt gccgcacttg ccagtccaac tacaggcacc agtaacgata
    22681 acggtgcaac ccgccaggtt tcatagcgtc ccagtaacgt cccccagatc ccataaccaa
    22741 caattgtcgc cacaaacgcc agatacatca gagacaagat ggtagtcata tcgatagtaa
    22801 ccagactgtg aatcatggtt gcggaaccat cgagaatcag cgaggcaaca aagaagggaa
    22861 tgattgggat taatgcgctc cagattacca gcgacatcac cgccggacgc gttgcgtgcg
    22921 acatgatctt tttattgaag atgttgccac acgcccaact aaatgctgcc gccagggtca
    22981 acataaagcc gagcatcgcc acatgctgac cgttcagact atcttcgatt aacaccagta
    23041 cgccaaaaat cgctaaggcg atccccgcca attgtttgcc atgcagtcgc tcaccgaaag
    23101 taaacgcgcc aagcacgata gtaaaaaacg cctgtgcctg taacaccagc gaagccagtc
    23161 cagcaggcat accaaagtta atggcgcaga acagaaaagc aaactgcgca aaactgatgg
    23221 ttaatccata ccccagcagc aaattcagtg gtactttcgg tcgtgcgaca aaaaagatgg
    23281 ccgggaaagc gaccagcata aagcgcaaac cggccagcat cagcggtggc atgttatgaa
    23341 gccccacttt gatgaccaca aaatttagcc cccatacaac cactaccagt agcgccaaca
    23401 ccccatcttt tcgcgacatt ctaccgcctc tgaatttcat cttttgtaag caatcaactt
    23461 agctgaattt acttttcttt aacagttgat tcgttagtca ccgtttacga cggcattaat
    23521 gcgcaaataa gtcgctatac ttcggatttt tgccatgcta ttcctttaca tctctaaaac
    23581 aaaacataac gaaacgcact gccggacaga caaatgaact tatccctacg acgctctacc
    23641 agcgcccttc ttgcctcgtc gttgttatta accatcggac gcggcgctac gctgccgttt
    23701 atgaccattt acttgagtcg ccagtacagc ctgagtgtcg atctaatcgg ttatgcgatg
    23761 acaattgcgc tcactattgg cgtcgttttt agcctcggtt ttggtatcct ggcggataag
    23821 ttcgacaaga aacgctatat gttactggca attaccgcct tcgctagcgg ttttattgcc
    23881 attcctttag tgaataacgt gacgctggtg gtgctctttt ttgcccttat taactgcgcc
    23941 tactccgttt ttgctaccgt gctgaaagcc tggtttgccg acaatctttc gtccaccagc
    24001 aaaacgaaga ttttctcaat caactacacc atgctcaaca ttggctggac catcggtccg
    24061 ccactcggca cgctgttggt gatgcagagc atcaatctgc ccttctggct ggcagctatc
    24121 tgttccgcgt tccccatgct tttcattcaa atttgggtaa agcgcagcga gaaaatcatc
    24181 gccacggaaa caggcagtgt ctggtcgccg aaagttttat tacaagataa agcactgttg
    24241 tggtttacct gctctggctt tctggcttct tttgttagcg gcgcatttgc ttcatgcatt
    24301 tcacaatatg tgatggtgat tgctgatggt gattttgccg aaaaggtggt cgcggttgtt
    24361 cttccggtga atgctgccat ggtggttacg ttgcaatatt ccgtgggccg ccgccttaac
    24421 ccggctaaca tccgcgcgct gatgacagca ggcaccctct gtttcgtcat cgggctggtc
    24481 ggttttattt tttccggcaa cagcctgcta ttgtggggta tgtcagctgc ggtatttact
    24541 gtcggtgaaa tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca
    24601 ggaatgaaag ccagctattt ttccgcccag tctttaggct ggcttggtgc tgcgattaac
    24661 ccattagtga gtggcatagt gcttaccagt ctgccgcctt tctcgctgtt tatcatctta
    24721 gcgttagtga tcgttgttgc gtgggtgctg atgttaaaag gaattcgtgc aagaccgtgg
    24781 gggcagcccg cgctttgttg atttaagtcg aacacaataa gaatttaatt cagccttcgt
    24841 ttaggttacc tctgctaata tctttctcat tgagatgaaa attaaggtaa gcgaggaaac
    24901 acaccacacc ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta
    24961 ctgggaataa ttttattttc tgggtttctg gccgcgtatt tcagccacaa atgggatgac
    25021 taatgaacgg agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc
    25081 ctgattttta tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga
    25141 tagatttgtg ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg
    25201 acacagtagc atcagttttc tcaatgaatg ttaaacggag ctcaaactcg gttaatcaca
    25261 ttttgttcgt caataaacat gcagcgattc cttccggttt gcttaccctc atacattgcc
    25321 ctgtccgctc ttccaatgac cacatccaga ggctcttcag gaaatgcacg actcacacca
    25381 gcagtcacgg taatgttgat atgcccttca gaatgtgtga tggcatggtt atcgactaac
    25441 tggcaaattc tgacacctgc acgacatgct tcttcatcat tagtcgcttt gacaataatg
    25501 ataaattctt cgcccccgta gcgataaacc gtttcgtaat cgcacgtcca actggctaag
    25561 taagttgcga gggtgcgtaa tactacatcg ccgattaaat gcccgtaggt atcattaacc
    25621 aatttaaatc ggtctatatc caacaacatt aaataaagat tcagaggttc agcattgcgt
    25681 aactgatgat cgaaggattc atcaagaacc cgacgacccg gcaatcccgt cagaacatcc
    25741 atattgctac ggaccgtaag caaataaatt ttgtaatcgg ttaatgccgc agtaaaagaa
    25801 agcaaccctt cctgaaaagc gtcgaaatgc gcgtcctgcc agtgattttc aacaatagcc
    25861 agcattaatt cccgaccaca gttatgcata tgttgatggg cagaatccat tagccgaacg
    25921 taaggtaatt catcgttatc gagtggcccc agatgatcaa tccaccgacc aaactggcac
    25981 agtccataag aatggttatc cgttatttct ggcttactgg catctctcgc gaccacgctg
    26041 tgaaacatac ttaccagcca ctggtagtgg gcatcgatag ccttattgag atttaacaag
    26101 atggcatcaa tttccgttgt cttcttgatc attgccactc ctttttcaca gttccttgtg
    26161 cgcgctattc taacgagaga aaagcaaaat tacgtcaata ttttcataga aatccggagt
    26221 tatgagcatc tctgagataa cattgtgatt taaaacaaaa tcagcagata aaaaagtatt
    26281 taattctata aattaactct gcattatcgc aattaaaagg atgacaaata gcataaccca
    26341 ataccctaat ggcccagtag ttcaggccat caggctaatt tatttttatt tctgcaactg
    26401 agtgacccga acgacggccg gcgcgctttt cttatccaga ctgccgctaa tgttgatcat
    26461 ctggtc
//
LOCUS       DAZKHK010000076        26454 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKHK010000076 DAZKHK010000000
VERSION     DAZKHK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.076"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            18..1487
                     /gene="gspA"
                     /locus_tag="ACHOS5_003064"
     CDS             18..1487
                     /gene="gspA"
                     /locus_tag="ACHOS5_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFP9777838.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTRVFT
                     AKQWRMIYRVLGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTAPLPVPAWLIPVTPV
                     AKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     SDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1489..1908
                     /gene="gspB"
                     /locus_tag="ACHOS5_003065"
     CDS             1489..1908
                     /gene="gspB"
                     /locus_tag="ACHOS5_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFP9777839.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2146..2457
                     /gene="rpsJ"
                     /locus_tag="ACHOS5_003066"
     CDS             2146..2457
                     /gene="rpsJ"
                     /locus_tag="ACHOS5_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9777840.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2490..3119
                     /gene="rplC"
                     /locus_tag="ACHOS5_003067"
     CDS             2490..3119
                     /gene="rplC"
                     /locus_tag="ACHOS5_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9777841.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3130..3735
                     /gene="rplD"
                     /locus_tag="ACHOS5_003068"
     CDS             3130..3735
                     /gene="rplD"
                     /locus_tag="ACHOS5_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9777842.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3732..4034
                     /gene="rplW"
                     /locus_tag="ACHOS5_003069"
     CDS             3732..4034
                     /gene="rplW"
                     /locus_tag="ACHOS5_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9777843.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4052..4873
                     /gene="rplB"
                     /locus_tag="ACHOS5_003070"
     CDS             4052..4873
                     /gene="rplB"
                     /locus_tag="ACHOS5_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9777844.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4890..5168
                     /gene="rpsS"
                     /locus_tag="ACHOS5_003071"
     CDS             4890..5168
                     /gene="rpsS"
                     /locus_tag="ACHOS5_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9777845.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5183..5515
                     /gene="rplV"
                     /locus_tag="ACHOS5_003072"
     CDS             5183..5515
                     /gene="rplV"
                     /locus_tag="ACHOS5_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9777846.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5533..6234
                     /gene="rpsC"
                     /locus_tag="ACHOS5_003073"
     CDS             5533..6234
                     /gene="rpsC"
                     /locus_tag="ACHOS5_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9777847.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6247..6657
                     /gene="rplP"
                     /locus_tag="ACHOS5_003074"
     CDS             6247..6657
                     /gene="rplP"
                     /locus_tag="ACHOS5_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9777848.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6657..6848
                     /gene="rpmC"
                     /locus_tag="ACHOS5_003075"
     CDS             6657..6848
                     /gene="rpmC"
                     /locus_tag="ACHOS5_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9777849.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6848..7102
                     /gene="rpsQ"
                     /locus_tag="ACHOS5_003076"
     CDS             6848..7102
                     /gene="rpsQ"
                     /locus_tag="ACHOS5_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9777850.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7267..7638
                     /gene="rplN"
                     /locus_tag="ACHOS5_003077"
     CDS             7267..7638
                     /gene="rplN"
                     /locus_tag="ACHOS5_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9777851.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7649..7963
                     /gene="rplX"
                     /locus_tag="ACHOS5_003078"
     CDS             7649..7963
                     /gene="rplX"
                     /locus_tag="ACHOS5_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9777852.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7978..8517
                     /gene="rplE"
                     /locus_tag="ACHOS5_003079"
     CDS             7978..8517
                     /gene="rplE"
                     /locus_tag="ACHOS5_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9777853.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8532..8837
                     /gene="rpsN"
                     /locus_tag="ACHOS5_003080"
     CDS             8532..8837
                     /gene="rpsN"
                     /locus_tag="ACHOS5_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9777854.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8871..9263
                     /gene="rpsH"
                     /locus_tag="ACHOS5_003081"
     CDS             8871..9263
                     /gene="rpsH"
                     /locus_tag="ACHOS5_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9777855.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9276..9809
                     /gene="rplF"
                     /locus_tag="ACHOS5_003082"
     CDS             9276..9809
                     /gene="rplF"
                     /locus_tag="ACHOS5_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9777856.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9819..10172
                     /gene="rplR"
                     /locus_tag="ACHOS5_003083"
     CDS             9819..10172
                     /gene="rplR"
                     /locus_tag="ACHOS5_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9777857.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10187..10690
                     /gene="rpsE"
                     /locus_tag="ACHOS5_003084"
     CDS             10187..10690
                     /gene="rpsE"
                     /locus_tag="ACHOS5_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9777858.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10694..10873
                     /gene="rpmD"
                     /locus_tag="ACHOS5_003085"
     CDS             10694..10873
                     /gene="rpmD"
                     /locus_tag="ACHOS5_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9777859.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10877..11311
                     /gene="rplO"
                     /locus_tag="ACHOS5_003086"
     CDS             10877..11311
                     /gene="rplO"
                     /locus_tag="ACHOS5_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9777860.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11319..12650
                     /gene="secY"
                     /locus_tag="ACHOS5_003087"
     CDS             11319..12650
                     /gene="secY"
                     /locus_tag="ACHOS5_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9777861.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12682..12798
                     /gene="rpmJ"
                     /locus_tag="ACHOS5_003088"
     CDS             12682..12798
                     /gene="rpmJ"
                     /locus_tag="ACHOS5_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9777862.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12945..13301
                     /gene="rpsM"
                     /locus_tag="ACHOS5_003089"
     CDS             12945..13301
                     /gene="rpsM"
                     /locus_tag="ACHOS5_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9777863.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13318..13707
                     /gene="rpsK"
                     /locus_tag="ACHOS5_003090"
     CDS             13318..13707
                     /gene="rpsK"
                     /locus_tag="ACHOS5_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9777864.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13741..14361
                     /gene="rpsD"
                     /locus_tag="ACHOS5_003091"
     CDS             13741..14361
                     /gene="rpsD"
                     /locus_tag="ACHOS5_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9777865.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14387..15376
                     /gene="rpoA"
                     /locus_tag="ACHOS5_003092"
     CDS             14387..15376
                     /gene="rpoA"
                     /locus_tag="ACHOS5_003092"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9777866.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15417..15800
                     /gene="rplQ"
                     /locus_tag="ACHOS5_003093"
     CDS             15417..15800
                     /gene="rplQ"
                     /locus_tag="ACHOS5_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9777867.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15907..16275
                     /gene="yhdN"
                     /locus_tag="ACHOS5_003094"
     CDS             15907..16275
                     /gene="yhdN"
                     /locus_tag="ACHOS5_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP9777868.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16286..16711
                     /gene="zntR"
                     /locus_tag="ACHOS5_003095"
     CDS             16286..16711
                     /gene="zntR"
                     /locus_tag="ACHOS5_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP9777869.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16767..16985
                     /gene="arfA"
                     /locus_tag="ACHOS5_003096"
     CDS             16767..16985
                     /gene="arfA"
                     /locus_tag="ACHOS5_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP9777870.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(16982..17395)
                     /gene="mscL"
                     /locus_tag="ACHOS5_003097"
     CDS             complement(16982..17395)
                     /gene="mscL"
                     /locus_tag="ACHOS5_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9777871.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17525..18901)
                     /gene="trkA"
                     /locus_tag="ACHOS5_003098"
     CDS             complement(17525..18901)
                     /gene="trkA"
                     /locus_tag="ACHOS5_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP9777872.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18923..20212)
                     /gene="rsmB"
                     /locus_tag="ACHOS5_003099"
     CDS             complement(18923..20212)
                     /gene="rsmB"
                     /locus_tag="ACHOS5_003099"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9777873.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWSHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20258..21205)
                     /gene="fmt"
                     /locus_tag="ACHOS5_003100"
     CDS             complement(20258..21205)
                     /gene="fmt"
                     /locus_tag="ACHOS5_003100"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9777874.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(21220..21729)
                     /gene="def"
                     /locus_tag="ACHOS5_003101"
     CDS             complement(21220..21729)
                     /gene="def"
                     /locus_tag="ACHOS5_003101"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9777875.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21859..22983
                     /gene="dprA"
                     /locus_tag="ACHOS5_003102"
     CDS             21859..22983
                     /gene="dprA"
                     /locus_tag="ACHOS5_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP9777876.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22955..23428
                     /gene="smg"
                     /locus_tag="ACHOS5_003103"
     CDS             22955..23428
                     /gene="smg"
                     /locus_tag="ACHOS5_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP9777877.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23457..23999
                     /gene="yrdD"
                     /locus_tag="ACHOS5_003104"
     CDS             23457..23999
                     /gene="yrdD"
                     /locus_tag="ACHOS5_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP9777878.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            24004..24576
                     /gene="tsaC"
                     /locus_tag="ACHOS5_003105"
     CDS             24004..24576
                     /gene="tsaC"
                     /locus_tag="ACHOS5_003105"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP9777879.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANCEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24581..25399
                     /gene="aroE"
                     /locus_tag="ACHOS5_003106"
     CDS             24581..25399
                     /gene="aroE"
                     /locus_tag="ACHOS5_003106"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9777880.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRLLLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25396..25653
                     /gene="yrdB"
                     /locus_tag="ACHOS5_003107"
     CDS             25396..25653
                     /gene="yrdB"
                     /locus_tag="ACHOS5_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP9777881.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQNEDDQGWVWLP"
     gene            complement(25629..26183)
                     /gene="yrdA"
                     /locus_tag="ACHOS5_003108"
     CDS             complement(25629..26183)
                     /gene="yrdA"
                     /locus_tag="ACHOS5_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9777882.1"
                     /translation="MSEVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
BASE COUNT         6753 a         6237 c         6904 g         6560 t
ORIGIN      
        1 ttaaaacagg aagttctatg tctacgagaa gagaagtcat tctttcctgg ttgcgtgaga
       61 aacgtcagac ctggcgtctg tgctatttgt tgggtgaggc tggaagtgga aaaacctggc
      121 tggcgcagca actacaaaaa gataaacatc gccgtgtgat tactttaagc ctcgttgttt
      181 cctggcaagg taaggccgca tggatcgtta ccgacgataa tgcggctgaa cagggctgcc
      241 gtgacagcgc ctggacgcga gatgagatgg cgggtcaatt actgcatgca cttcatcgaa
      301 ctgatagtcg ctgccctctt attattattg aaaatgctca cctgaatcac cgccgcatac
      361 ttgatgatct ccaacgcgcg atatctctta ttcctgacgg tcaatttttg ctgatcggca
      421 gacccgatcg aaaagtcgaa cgtgatttta aaaaacaggg cattgaactt gtctcgacag
      481 gtcgtctgac ggagcacgag cttaaggcaa gcatccttga aggtcagaat attgaccagc
      541 ccgatctcct tttaaccgcc agagttctga aacggatagc tttattatgt cggggagatc
      601 gcagaaagct ggcgcttgcc ggcgaaacaa ttagcttatt acagcaagcc gagcagaccc
      661 gcgttttcac tgcaaaacaa tggcgaatga tttaccgcgt attaggtgat aaccgccccc
      721 ggaaaatgca gcttgccgtg gtgatgagtg gaaccattat cgcacttacc tgtggttggc
      781 tactgctgtc ctctttcaca gctccgttac ccgttcctgc gtggctgata cccgtaacgc
      841 cagtagctaa acaagatatg actaaggata ttgcccatgt ggtgatgcgc gatagtgagg
      901 cgctaagcgt gttatacggc gtatgggggt acgaggtccc agccgattcg gcttggtgcg
      961 atcaggcagt aagagcgggg ctggtatgta aaagtgggaa tgcttcttta caaacattgg
     1021 tggatcagaa tttgccctgg atcgcatcgc ttaaagtcgg ggacaaaaaa ttgcctgttg
     1081 tggtcgttcg cgtcggtgat gctaccgttg atgtactcgt agggcagcaa acctggacgc
     1141 tgacacataa atggtttgag ttggtatgga cgggtgatta tcttctgttg tggaaaatgt
     1201 cgccggaagg ggagagtacc attacgcgtg atagcagcga ggaagagatt ctctggctgg
     1261 aaacgatgtt aaaccgcgca ttgcatattt cgacagaatc ttcggcagag tggcgtccct
     1321 tgttggtaga aaaaataaag caatttcaaa aaagccatca cttgaaaagt gacggtgttg
     1381 ttggcttttc aacgttagta catctctggc aggtggcggg ggagagtgcc tacttatatc
     1441 gggatgaagc aaatatttcc ccagaaacaa cagtgaaggg gaaatgacat gtttgaattc
     1501 tatatcgccg cgcgtgagca gaaagaaaca ggtcaccctg gtatcttctc ccgacaaaaa
     1561 cacagcacca taatatatgt catttgttta ttgctgattt gcctgtggtt tgcaggaatg
     1621 gtgttggttg gtgggtatgc ccggcagcta tgggcacttt ggatagtaaa agcagaagtc
     1681 actgtagatg ctgaaacgcc cgcttttaaa caatcaacac aacactattt tttcaaaaaa
     1741 cagccactcc ccgtcgtcga atccgtcgag gaggaagatg aaccaggcgt cgcggttgag
     1801 aatgccccct cttcttctga agacgaagaa aatactgtag aagaaagcga cgagaaagcc
     1861 ggtttaagag agcgtgtcaa aaatgcgctg aacgaacttg agagataacc caaaggttgt
     1921 ttactttact agcaatacgc ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt
     1981 cgtagttgac agcaggttca atctgaacct cagttagccg aatttggcta cctaacaatg
     2041 ctcccaatcg gggagctacg taagaacggt tacactctcc catcaatcgt aatgggtctg
     2101 aggagtaatc attttcgttt ataaaataat tggagctctg gtctcatgca gaaccaaaga
     2161 atccgtatcc gcctgaaagc gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc
     2221 gtcgagactg ctaagcgcac tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc
     2281 aaagagcgct tcactgttct gatctccccg cacgtcaaca aagacgcgcg cgatcagtac
     2341 gaaatccgta ctcacttgcg tctggttgac atcgttgagc caaccgagaa aaccgttgat
     2401 gctctgatgc gtctggatct ggctgccggt gtagacgtgc agatcagcct gggttaatca
     2461 ggtcattgag cgattgagag gttgaaacaa tgattggttt agtcggtaaa aaagtgggta
     2521 tgacccgtat cttcacagaa gacggcgttt ctatcccagt aaccgtaatc gaagttgaag
     2581 caaaccgcgt tactcaggtt aaagacctgg ctaacgatgg ctaccgtgct attcaggtga
     2641 ccaccggtgc taaaaaagct aaccgtgtga ccaagcctga agctggccac ttcgctaaag
     2701 ctggcgtaga agctggccgt ggtctgtggg aattccgcct ggctgaaggc gaagagttca
     2761 ctgtaggtca gagcattagc gttgaactgt ttgctgacgt taaaaaagtt gacgtaactg
     2821 gcacctctaa aggtaaaggt ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg
     2881 acgctactca cggtaactcc ttgtctcacc gcgttccggg ttctatcggt cagaaccaga
     2941 ctccgggcaa agtgttcaaa ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa
     3001 ccgttcagag ccttgacgta gtacgcgttg acgctgagcg caacctgctg ctggttaaag
     3061 gcgctgtccc gggtgcaacc ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag
     3121 gagatagcaa tggaattagt attgaaagac gcgcagagcg cgctgactgt ttccgaaact
     3181 accttcggtc gtgatttcaa cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct
     3241 ggtgctcgtc agggtactcg tgctcagaag actcgtgctg aagtaactgg ttccggtaaa
     3301 aaaccgtggc gccagaaagg caccggccgt gcgcgttctg gttctattaa gagcccgatc
     3361 tggcgttctg gtggcgtgac ctttgctgct cgtccgcagg accacagtca aaaagttaac
     3421 aagaagatgt accgcggcgc gctgaaaagc atcctgtccg aactggtacg tcaggatcgt
     3481 ctgatcgttg tcgagaagtt ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa
     3541 ctgaaagaca tggctctgga agatgtgctg atcatcaccg gtgagctgga cgagaacctg
     3601 ttcctggctg cgcgcaacct gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg
     3661 gttagcctga tcgccttcga caaagtcgta atgactgctg atgctgttaa gcaagttgag
     3721 gagatgctgg catgattcgt gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt
     3781 ctgaaaaagc gtctactgcg atggaaaaat ccaacaccat cgtactcaaa gttgctaaag
     3841 acgcgaccaa agcagaaatc aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag
     3901 tcgttaacac cctggtagtt aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc
     3961 gtagcgactg gaaaaaagct tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg
     4021 gcggcgctga gtaagtcgga ggagtaatac aatggcagtt gttaaatgta aaccgacatc
     4081 tccgggtcgt cgccacgtag ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt
     4141 tgctccgttg ctggaaaaaa acagcaaatc cggtggtcgt aacaacaatg gccgtatcac
     4201 cactcgtcat atcggtggtg gccacaagca ggcttaccgt attgttgact tcaaacgcaa
     4261 caaagacggt atcccggcag ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa
     4321 catcgcgctg gttctgtaca aagacggtga acgccgttac atcctggccc ctaaaggcct
     4381 gaaagctggc gaccagattc agtctggcgt tgatgctgca atcaaaccag gtaacaccct
     4441 gccgatgcgc aacatcccgg ttggttctac tgttcataac gtagaaatga aaccaggtaa
     4501 aggcggtcag ctggcacgtt ccgctggtac ttacgttcag atcgttgctc gtgatggtgc
     4561 ttatgtcacc ctgcgtctgc gttctggtga aatgcgtaaa gtagaagcag actgccgtgc
     4621 aactctgggc gaagttggca atgctgagca tatgctgcgc gttctgggta aagcaggtgc
     4681 tgcacgctgg cgtggtgttc gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca
     4741 cccacatggt ggtggtgaag gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt
     4801 tcagaccaaa ggtaagaaga cccgcagcaa caagcgtact gataaattca tcgtacgtcg
     4861 ccgtagcaaa taattttaga ggataagcca tgccacgttc tctcaagaaa ggtcctttta
     4921 ttgacctgca cttgctgaag aaggtagaga aagcggtgga aagcggagac aagaagcccc
     4981 tgcgcacttg gtcccgtcgt tcaacgatct ttcctaacat gatcggtttg accatcgctg
     5041 tccataatgg tcgtcagcac gttccggtat ttgtaaccga cgaaatggtc ggtcacaaac
     5101 tgggtgaatt cgcaccgact cgtacttatc gcggccacgc tgctgataaa aaagcgaaga
     5161 agaaataagg taggaggaag agatggaaac tatcgctaaa catcgccatg ctcgttcttc
     5221 tgctcagaag gttcgccttg ttgctgacct gattcgcggt aagaaagtgt cgcaggctct
     5281 ggatattttg acctacacca acaagaaagc ggctgtactg gtcaagaaag ttctggaatc
     5341 tgccattgct aacgctgaac acaacgatgg cgctgacatt gacgatctga aagttacgaa
     5401 aattttcgta gacgaaggcc cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc
     5461 agatcgcatc ctgaagcgca ccagccacat cactgtggtt gtgtccgatc gctgagactc
     5521 tggagactag caatgggtca gaaagtacat cctaatggta ttcgcctggg tattgtaaaa
     5581 ccatggaact ctacctggtt tgcgaacacc aaagaattcg ctgacaacct ggacagcgat
     5641 tttaaagtac gtcagtacct gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt
     5701 atcgagcgtc cggctaagag catccgtgta accattcaca ctgctcgccc gggtatcgtt
     5761 atcggtaaaa aaggtgaaga cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc
     5821 gttcctgcac agatcaacat cgccgaagtt cgtaagcctg aactggacgc aaaactggtt
     5881 gctgacagca tcacttctca gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt
     5941 gctgtacaga acgcaatgcg tctgggcgct aaaggtatta aagttgaagt tagcggccgt
     6001 ctgggcggcg cggaaatcgc acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac
     6061 actctgcgtg ctgacatcga ctacaacacc tctgaagcgc acaccactta cggtgtaatc
     6121 ggcgttaaag tgtggatctt caaaggcgag atcctgggtg gtatggctgc tgttgaacaa
     6181 ccggaaaaac cggctgctca gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg
     6241 tcgctgatgt tacaaccaaa gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc
     6301 ggtctggcgc agggtacgga tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt
     6361 ggtcgtctga ctgcacgtca gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag
     6421 cgtcaaggta agatctggat ccgtgtgttc ccggacaaac cgatcactga aaagccgctg
     6481 gcagtgcgta tgggtaaagg taaaggtaac gtggagtatt gggttgcctt gattcagccg
     6541 ggtaaagtcc tgtatgaaat ggacggtgtt ccggaagagc tggcccgtga agcattcaag
     6601 ctggcagcag cgaaactgcc gattaaaacc acctttgtaa ctaagacggt gatgtaatga
     6661 aagcaaaaga gctgcgtgag aagagcgttg aagagctgaa caccgagctg ctgaacctgc
     6721 tgcgtgagca gttcaacctg cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc
     6781 tgttgaagca agtgcgtcgc gatgtcgcac gcgttaagac tttactgaac gagaaggcgg
     6841 gtgcgtaatg accgataaaa tccgtactct gcaaggtcgc gttgttagcg acaaaatgga
     6901 gaaatccatt gttgttgcta tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat
     6961 caagcgtacg accaaactgc acgtacatga cgagaacaac gaatgcggta tcggtgacgt
     7021 ggttgaaatc cgcgaatgcc gtccgctgtc caagactaaa tcctggacgc tggttcgcgt
     7081 tgtagagaaa gcggttctgt aatacagtac actctctcga tacgaataaa cggctcagaa
     7141 atgagccgtt tattttttct acccatatcc ttgaagcggt gttataatgc cgcgccctcg
     7201 atatggggat ttttaacgac ctgattttcg ggtctcagta gtagttgaca ttagcggagc
     7261 actaaaatga tccaagaaca gactatgctg aacgtcgccg acaactccgg tgcacgtcgc
     7321 gtaatgtgta tcaaggttct gggtggctcg caccgtcgct acgcaggcgt aggcgacatc
     7381 atcaagatca ccatcaaaga agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg
     7441 aaggcggtag tggtgcgcac caagaagggt gttcgtcgcc cggacggttc tgtcattcgc
     7501 ttcgatggta atgcttgtgt tcttctgaac aacaacagcg agcagcctat cggtacgcgt
     7561 atttttgggc cggtaactcg tgagcttcgt agtgagaagt tcatgaaaat tatctctctg
     7621 gcaccagaag tactctaagg agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt
     7681 tatcgtgtta accggtaaag ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc
     7741 cggcaaggtc attgttgaag gtatcaacct ggttaagaaa catcagaagc cggttccggc
     7801 cctgaaccaa ccgggtggca tcgttgaaaa agaagccgct attcaggttt ccaacgtagc
     7861 aatcttcaat gcgacaaccg gcaaggctga ccgtgtaggc tttagattcg aagacggtaa
     7921 aaaagtccgt ttcttcaagt ctaacagcga aactatcaag taatttggag tagtacgatg
     7981 gcgaaactgc atgattacta caaagacgaa gtagttaaaa aactcatgac tgagtttaac
     8041 tacaattctg tcatgcaagt ccctcgggtc gagaagatca ccctgaacat gggtgttggt
     8101 gaagcgatcg ctgacaaaaa actgctggat aacgcagcag cagacctggc agcaatctcc
     8161 ggtcaaaaac cgctgatcac caaagcacgc aaatctgttg caggcttcaa aatccgtcag
     8221 ggctatccga tcggctgtaa agtaactctg cgtggcgaac gcatgtggga gttctttgag
     8281 cgcctgatca ctattgctgt acctcgtatc cgtgacttcc gtggcctgtc cgctaagtct
     8341 ttcgacggtc gtggtaacta cagcatgggt gtccgtgagc agatcatctt cccagaaatc
     8401 gactacgata aagtcgaccg cgttcgtggt ttggatatta ccattaccac tactgcgaaa
     8461 tctgacgaag aaggccgcgc tctgctggct gcctttgact tcccgttccg caagtaaggt
     8521 agggttacta aatggctaag caatcaatga aagcacgcga agtaaaacgc gtagctttag
     8581 ctgataaata cttcgcgaaa cgcgctgaac tgaaagcgat catctctgat gtgaacgctt
     8641 ccgacgaaga tcgttggaac gctgttctca agctgcagac tctgccgcgt gattccagcc
     8701 cgtctcgtca gcgtaaccgc tgccgtcaaa caggtcgtcc gcatggtttc ctgcggaagt
     8761 tcgggttgag ccgtattaag gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga
     8821 aaaaggctag ctggtaattg tcaccaattg aatcacggga gtaaagacag atgagcatgc
     8881 aagatccgat cgcggatatg ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg
     8941 cggtcaccat gccttcctcc aagctgaaag tggcaatcgc caacgtgctg aaggaagaag
     9001 gttttattga agattttaaa gttgaaggcg acaccaagcc tgaactggaa cttactctga
     9061 agtatttcca gggcaaagct gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc
     9121 gcatctataa acgtaaagat gagctgccga aagttatggc gggtctgggt atcgcagttg
     9181 tttctacctc taaaggtgtt atgactgatc gtgcagcgcg ccaggctggt cttggtggcg
     9241 aaattatctg ctacgtagcc taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg
     9301 tcgttgttcc tgccggcgtt gacgtaaaaa tcaacggtca ggttattacg atcaaaggta
     9361 aaaacggcga gctgactcgt actctcaacg atgctgttga agttaaacat gcagataata
     9421 ccctgacctt cggtccgcgt gatggttacg cagacggttg ggcacaggct ggtaccgcgc
     9481 gtgccctgct gaactcaatg gttatcggtg ttaccgaagg cttcactaag aagctgcagc
     9541 tggttggtgt aggttaccgt gcagcggtta aaggcaatgt gattaacctg tctctgggtt
     9601 tctctcatcc tgttgaccat cagctgcctg cgggtatcac tgctgaatgt ccgactcaga
     9661 ctgaaatcgt gctgaaaggc gctgataagc aggtgatcgg ccaggttgca gcggatctgc
     9721 gtgcctaccg tcgtcctgag ccttacaaag gcaagggtgt tcgttacgcc gacgaagtcg
     9781 tgcgtaccaa agaggctaag aagaagtaag gtaacactat ggataagaaa tctgctcgta
     9841 tccgtcgtgc gacccgcgca cgccgcaagc tccaggagct gggcgcaact cgcctggtgg
     9901 tacatcgtac cccgcgtcac atttacgcac aggtaattgc accgaacggt tctgaagttc
     9961 tggtagctgc ttctactgta gaaaaagcta tcgctgaaca actgaagtac accggtaaca
    10021 aagacgcggc tgcagctgtg ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca
    10081 aagatgtatc ctttgaccgt tccgggttcc aatatcatgg tcgtgtccag gcactggcag
    10141 atgctgcccg tgaagctggc cttcagttct aaggtagagg tgtaagatgg ctcacatcga
    10201 aaaacaagct ggcgaactgc aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt
    10261 taaaggtggt cgtattttct ccttcacagc tctgactgta gttggcgatg gtaacggtcg
    10321 cgttggtttt ggttacggta aagcgcgtga agttccagca gcgatccaga aagcgatgga
    10381 aaaagcccgt cgcaatatga ttaacgtcgc gctgaataac ggcactctgc aacaccctgt
    10441 taaaggtgtt cacacgggtt ctcgcgtatt catgcagccg gcttccgaag gtaccggtat
    10501 catcgccggt ggtgcaatgc gcgccgttct ggaagtcgct ggggttcata acgttctggc
    10561 taaagcgtat ggttccacca acccgattaa cgtggttcgt gcaactatcg atggcctgga
    10621 aaatatgaat tctccagaaa tggtcgctgc caagcgtggt aaatccgttg aagaaattct
    10681 ggggaaataa accatggcaa agactattaa aattactcaa acccgcagtg caatcggtcg
    10741 tctgccgaaa cacaaggcaa cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt
    10801 agagcgcgag gatactcctg ctattcgcgg tatgatcaac gcggtttcct tcatggttaa
    10861 agttgaggag taagagatgc gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc
    10921 gggtaaacgc ctgggtcgtg gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca
    10981 caaaggtcag aaatctcgtt ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat
    11041 gcctctgtac cgtcgtctgc cgaaattcgg cttcacttct cgtaaagcag cgattacagc
    11101 cgaagttcgt ctgtctgact tggctaaagt agaaggcggt gttgtagacc tgaacacgct
    11161 gaaagcggct aacattatcg gtatccagat cgagttcgcg aaagtgatct tggctggcga
    11221 agtaacgact ccggtaactg ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat
    11281 cgaagctgct ggcggtaaaa tcgaggaata agtagcagat ggctaaacaa ccgggattag
    11341 attttcaaag tgccaaaggt ggcttaggcg agctgaaacg cagactgctg tttgttatcg
    11401 gtgcgctgat tgtgttccgt attggctctt ttattccgat ccctggtatt gatgccgctg
    11461 tacttgccaa actgcttgag caacagcgag gcaccatcat tgagatgttt aacatgttct
    11521 ctggtggtgc tctcagccgt gcttctatct ttgctctggg gatcatgccg tatatttcgg
    11581 cgtcgatcat tatccagtta ctgacggtgg ttcatccaac gttggcagaa attaagaaag
    11641 aaggggagtc tggtcgtcgt aagatcagcc agtacacccg ctacggtact ctggtgctgg
    11701 caatattcca gtcgatcggt attgctactg gtctgccgaa tatgcctggt atgcaaggcc
    11761 tggtgattaa cccgggcttt gcattctact tcaccgctgt tgtaagtctg gtcacaggaa
    11821 ccatgttcct gatgtggttg ggcgaacaga tcactgaacg aggtatcggc aacggtattt
    11881 caatcattat cttcgccggt attgtcgcgg gactcccgcc agccattgcc catactatcg
    11941 agcaagcgcg tcaaggcgac ctgcacttcc tcgtgttgct gttggttgca gtattagtat
    12001 ttgcagtgac gttctttgtt gtatttgttg agcgtggtca acgccgcatt gtggtaaact
    12061 acgcgaaacg tcagcaaggt cgtcgtgtct atgctgcaca gagcacacat ttaccgctga
    12121 aagtgaatat ggcgggggta atcccggcaa tcttcgcttc cagtattatt ctgttcccgg
    12181 cgaccatcgc gtcatggttc gggggcggta ctggttggaa ctggctgaca acaatttcgc
    12241 tgtatttgca gcctgggcaa ccgctttatg tgttactcta tgcgtctgca atcatcttct
    12301 tctgtttctt ctacacggcg ttggttttca acccgcgtga aacagcagat aacctgaaga
    12361 agtccggtgc atttgtacca ggaattcgtc cgggagagca aacggcgaag tatatcgata
    12421 aagtaatgac ccgcctgacc ttggttggtg cgctgtacat tacctttatc tgcctgatcc
    12481 cggagttcat gcgtgatgca atgaaagtac cgttctactt cggtgggacc tcactgctta
    12541 tcgttgttgt cgtgattatg gactttatgg ctcaagtgca aactctgatg atgtccagtc
    12601 agtatgagtc tgcattgaag aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc
    12661 gagaagttac ggagagtaaa aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac
    12721 tgcaaaatcg ttaagcgtga tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat
    12781 aaacagcgcc aaggctgatt ttttcgcata tttttcttgc aaagttgggt tgagctggct
    12841 agattagcca gccaatcttc tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg
    12901 gcttttcagc atggaacgta catattaaat agtaggagtg catagtggcc cgtatagcag
    12961 gcattaacat tcctgatcat aagcatgccg taatcgcatt aacttcgatt tatggcgtcg
    13021 gcaagacccg ttctaaagcc atcctggctg cagcgggtat cgctgaagat gttaagatca
    13081 gtgagctgtc tgaaggacaa atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg
    13141 aaggtgatct gcgccgtgaa atcagcatga gcatcaagcg cctgatggat cttggttgct
    13201 atcgcggttt gcgtcatcgt cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg
    13261 cacgtacccg taagggtccg cgcaaaccga tcaagaaata atcggggtga ttgaataatg
    13321 gcaaaggcac caattcgtgc acgtaaacgt gtaagaaaac aagtctctga cggcgtggct
    13381 catatccatg cttctttcaa caacaccatc gtgactatca ctgatcgtca gggtaacgcg
    13441 ttgggttggg caacagccgg tggttccggt ttccgtggtt ctcgcaaatc cactccgttt
    13501 gcagcacagg ttgcagcaga gcgttgcgct gacgccgtga aagaatacgg catcaagaat
    13561 ctggaagtta tggttaaagg tccgggtcca ggccgcgaat ctactattcg tgctctgaac
    13621 gccgcaggtt tccgcatcac taacattact gatgtgactc cgatccctca taacggttgt
    13681 cgtccgccga aaaaacgtcg cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa
    13741 atggcaagat atttgggtcc taagctcaag ctgagccgtc gtgagggcac cgacttattc
    13801 cttaagtctg gcgttcgcgc gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag
    13861 cacggtgcgc gtaaaccgcg tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa
    13921 gttcgccgta tctatggtgt gctggagcgt cagttccgta actactacaa agaagcagca
    13981 cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc tggaaggtcg tctggacaac
    14041 gttgtatacc gtatgggctt cggtgccact cgtgcagaag cacgtcagct ggttagccat
    14101 aaagcaatta tggtaaacgg tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat
    14161 gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag
    14221 ctggctgagc agcgtgaaaa gccaacctgg ctggaagttg atgctggcaa gatggaaggt
    14281 acgtttaagc gtaagccgga gcgttctgat ctgtctgcgg acattaacga acacctgatc
    14341 gtcgagcttt actccaagta aagcttagta ccaaagagag gacacaatgc agggttctgt
    14401 gacagagttt ctaaaaccgc gcctggttga tatcgagcaa gtgagttcga cgcacgccaa
    14461 ggtgaccctt gagcctttag agcgtggctt tggccatact ctgggtaacg cactgcgccg
    14521 tattctgctc tcatcgatgc cgggttgcgc ggtgaccgag gttgagattg atggtgtact
    14581 acatgagtac agcaccaaag aaggcgttca ggaagatatc ctggaaatcc tgctcaacct
    14641 gaaagggctg gcggtgagag ttcagggcaa agatgaagtt attcttacct tgaataaatc
    14701 tggcattggc cctgtgactg cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa
    14761 gccgcagcac gtgatctgcc acctgaccga tgagaacgcg tctattagca tgcgtatcaa
    14821 agttcagcgc ggtcgtggtt atgtgccggc ttctacccga attcattcgg aagaagatga
    14881 gcgcccaatc ggccgtctgc tggtcgacgc atgctacagc cctgtggagc gtattgccta
    14941 caatgttgaa gcagcgcgtg tagaacagcg taccgacctg gacaagctgg tcatcgaaat
    15001 ggaaaccaac ggcacaatcg atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc
    15061 tgaacaactg gaagctttcg ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga
    15121 gaaaccagag ttcgatccga tcctgctgcg ccctgttgac gatctggaat tgactgtccg
    15181 ctctgctaac tgccttaaag cagaagctat ccactatatc ggtgatctgg tacagcgtac
    15241 cgaggttgag ctccttaaaa cgcctaacct tggtaaaaaa tctcttactg agattaaaga
    15301 cgtgctggct tcccgtggac tgtctctggg catgcgcctg gaaaactggc caccggcaag
    15361 catcgctgac gagtaaccgg atcacaggtt aaggttttac tgagaaggat aaggtcatgc
    15421 gccatcgtaa gagtggtcgt caactgaacc gcaacagcag ccatcgccag gctatgttcc
    15481 gcaatatggc aggttcactg gttcgtcatg aaatcatcaa gacgactctg cctaaagcga
    15541 aagagctgcg ccgcgtagtt gagccgctga ttactcttgc caagactgat agcgttgcta
    15601 atcgtcgtct ggcattcgcc cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg
    15661 aactgggccc gcgtttcgcg agccgtgccg gtggttacac tcgtattctg aagtgtggct
    15721 tccgtgcagg cgacaatgcg ccgatggctt acatcgagct ggttgatcgt tcagagaaag
    15781 cagaagctgc tgcagagtaa tctgaagcaa cgtaaaaaaa cccgccccgg cgggtttttt
    15841 tatacccgta gcatccccac ttatctacaa tagctgtact ctttttgttc atcccctgga
    15901 gtatttatgt ggttacttga ccagtgggca gagcgtcata tagcagaagc gcaagcgaaa
    15961 ggtgagtttg ataacctggt aggtagcggc gaaccattga tactggatga tgattctcat
    16021 gtgccaccgg aattacgtgc ggggtatcgc ttgctgaaga atgctggttg cttaccgcca
    16081 gaacttgagc ataggaggga agcaatacag cttctggata ttctcaaagg tatccgtcat
    16141 gatgatccgc aatatcaaga ggttagtcgt cgattgtcat tactggaatt gaagctgcga
    16201 caagctggat tgagtaccga ttttttacgc ggcgattatg ctgacaagtt gttgaacaaa
    16261 atcaacgata actagtggag tatgtatgta tcgcattggt gagctggcaa aaatggcgga
    16321 agtaacaccc gacacgattc gttattacga aaagcagcag atgatggagc atgaagtacg
    16381 caccgaaggt gggtttcgcc tgtataccga aagcgatctc cagcgattga aatttatccg
    16441 ccatgccaga caactaggtt tcagtctgga gtcgatccgc gagttgctgt cgatccgtat
    16501 cgatcctgaa caccatacct gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga
    16561 agtcgaagct cgtatagccg agttgcagag tatgcagcgt tccttgcaac gccttaatga
    16621 tacctgttgt gggaccgccc atagcagtgt ttattgctcg attcttgaag ctcttgaaca
    16681 aggggcgagt ggcgttaaga gtggctgttg attttttgca ctggcaggat tacactcgcg
    16741 ccgttaaata accaattgga gtttttatga gccgatatca gcatactaaa gggcagataa
    16801 aggataatgc gatagaagca ttactacatg atcccttatt ccgacagcga gtagagaaaa
    16861 ataagaaagg gaaaggcagt tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg
    16921 aggccagtgg caagaaagta aatcactttt ttaccactgg ccttctgctt tcagacgctt
    16981 gttaagagcg gttattctgc tctttcagca aatcacgaat ttctgtcagt aatacttctt
    17041 ctttagttgg tgcaggtgtt gctgctgctg gttcttcttt tttccgattc agtttgttga
    17101 ttagcttaat cgccataaag atggcaaagg ccactatcag aaaatcaaag acgttttgaa
    17161 tgaagacacc gtaatgcatc acaacagcag ggatatcccc ctgcgcatcg cgtagcgtga
    17221 cagcaaactg tttgaaatcg atcccgccaa ttaataaccc cagaggtggc ataatgatat
    17281 cggcaaccag tgaagataca atcttcccga atgccgcacc gataatgaca cccaccgcca
    17341 aatccaccac gttcccgcgc atcgcaaatt cgcgaaattc tttaataatg ctcatgttat
    17401 tctccctatg aagccgacaa tcataagtct aacaaatgtt aagctatttt cctgccagga
    17461 gtgaatttaa ttaatccctg ataatcaaag ggaaataata aggcgtcatt agacgcctta
    17521 ttaattacaa gaagaaagga cttggctgga agagtctttc gacgtcggta ataaactttt
    17581 tatctgtgag gaacataatt acgtgatcgc cttgctcaat gcgcagattg tcattggcaa
    17641 tcatcacatc gtttccacgt accaccgctc caataatcgt tcctggcggt agcttgattt
    17701 cgtcaatgac tctgccgaca acgcgtgagg tgctttcatc accgtgagca acggcttcaa
    17761 tagcttctgc tacgccgcgg cgtaatgagg aaacaccaac aatatctgct tttcgcacat
    17821 ggctaagcaa cgcagaaata gttgcttgtt gtggtgaaat cgcaatatcg atcacgctcc
    17881 cctgaaccag atccacataa gcgcgacgtt ggatcaatac catcaccttt ttcgccccca
    17941 tacgtttggc aagcatggcg gacatgatat tggcctcgtc atcgttggtg acggcaataa
    18001 acagatcaac ttgatcgata tgttcttcgg ccagcagttc ttgatccgac gcatcaccaa
    18061 aaaagacgat cgtattctgc aacttttccg ccagttcggc agcgcgctgc tgattacgtt
    18121 cgatgagttt aacgctgtaa tctttttcca gacgacgcgc cagccctgca ccgatattac
    18181 cgccaccaac cagcatgatc cgcttatacg gtttttccag tcgctgtaat tcactcatca
    18241 ccgcgcggat atgctgtgaa gcggcaataa agaacacttc atcaccagct tcaacaatgg
    18301 tcgaaccttg cggacgaatg gggcgatcgt ggcggaaaat tgctgccaca cgagtatcga
    18361 tatgtggcat atgttcgcgc atggtcgaaa gtgcattacc aatcagcggg ccgccataat
    18421 aggctttaac cacagccagg ctgactttac cctcagcgaa gttcaccacc tgcaatgcgc
    18481 cgggatactc aatcagtcgg taaatattat cgataaccaa ctgctctggt gcgatcagat
    18541 gatcaatcgg tacagcatct gaatgaaata gcttatcggc atcgcgcacg tagtctggtg
    18601 agcggatacg agcgatgcga ttaggggtgt tgaaaagtga gtaggctacc tggcaggcaa
    18661 ccatattggt ttcatctgaa ctggttacag caaccagcat atcggcgtcg tcggcacctg
    18721 cctcccgcaa tacgcgtgga tgagagccat gcccctgcac gactcgcagg tcaaatttat
    18781 cctgtaaggt ccgcagacgc tcaccgttgg tatcgaccac agtaatatcg ttgttctcgc
    18841 caaccaggtt ttccgccagt gtgccgccaa cctggccggc acccagaatg ataattttca
    18901 tcagtcgcga cccgttatct catcactttt tgattagctt agcgtaaaag aagccgtcgc
    18961 cctcttcggc accaggcaga ttttgcttac ccggttgctc tggtgttcct gtttcgcaaa
    19021 gttcggcatc agcggtacgt tgcaaaaagg ctttaatctg caggctattc tcttccggta
    19081 acaccgaaca ggtggcatag accagggttc ctccggattt taaatgcgac cagatggcgt
    19141 cgagaatttc agactgcaac tgcgcgagtt ctgggatatc gcgatcgcgg cgtaaccatt
    19201 taatatccgg atggcgacga attacaccag ttgccgaaca aggtgcatct aataaaatgc
    19261 gatcgaactg ttgctcacca caccattggg aagggtaacg gccatcacct tgttttacgg
    19321 ttgccttcat accaaggcgt tttaaattgt cgtaaacgcg agagaggcgc tgctcatcaa
    19381 tatcgaccgc cagaacttgc gcctctggag caacctcaag aatatgcgtt gttttaccgc
    19441 ctggggcggc gcaaagatcc agaatgtgtt caccattttg tggagcaagc caggtcatgc
    19501 aaccttgtgc tgaagcatcc tgaacggtaa cccatccttc ttcaaaaccg ggtagcgcat
    19561 gaacaggtgc aggtgtttcc agctgtacag catcagggta atccgcatgc gggaaacctt
    19621 tcattcctgc ttcatccagc aatgcaagcc agctatcgcg ggaatgatgc gtacgattaa
    19681 cgcgcagcca cattggcgga cgctggttat tggcttcgac gatggattgc cactgctcgg
    19741 gatacgcctt ttgcagacgc ttcagcaacc aggaaggatg cagataacgt gcatcactgg
    19801 cattaaactc ggctaataac tcatcttgct gacgctggaa ctgacgtaat acaccgttaa
    19861 tcaatccctt aagttgcggg cgcttaattg caactgcgcc ttcaaccgtt tcagccagcg
    19921 cggcatgagg tggaatgcgg gtataaagca gttgatacaa accaaccata atcaggtaat
    19981 gaacagtacg ctgtttgcct gtcatcggac gggccattag cttattaatc agccagtcta
    20041 actgtgagag cgtacgcagt acgccaaagc acaactcttg aagaagtgct ttgtctttat
    20101 cagaaacttt ttgctggagc ggtggcagaa tgttgcttaa tgattgccct tgctcgacga
    20161 cttgttcaac ggcctgggcc gccatgctac gtaaattacg ttgttttttc ataagtataa
    20221 aaatgcccgg caagaccggg cttagaagag tggactatca ggccagacgg ttgcccggaa
    20281 caaaccattc ccgacgagaa ttcaggaggt cttgtgcgct catcgctttc ttacccgcag
    20341 gttgcaacga gagcagattc aggatgccat cacctgtcgc aacctgaatg ccttgtttat
    20401 tggcttcaag gattgttcct ggcgcagcct ttgttgtcgt atcgatgaca gatgctttcc
    20461 agactttaac cggctgccct tcaatttcca gccagctcat tggccatgga ttgaaagcgc
    20521 gaatgcaacg ttcaagctgt gctgccgaaa gtgaccagtc aatacgcgct tcttctttac
    20581 tcaacttttc ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc gccgtgccgt
    20641 ctgccagttg tttcaacgta gtaataagcc cttgtgggcc aagctctgcc agtttgtcgt
    20701 acagcgtacc actggtatct tctgcagtaa tcgggcagga gagcttataa agcatgtcac
    20761 cggtgtctaa accaacatcc atttgcataa tggtcacacc agtttctgca tcacccgccc
    20821 atagtgagcg ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa ccatgaacgt
    20881 tgatacagcc aagacgcggc atctccagca cagctttagg cagaattaaa ccataggcga
    20941 cgacgaccat aacatcagcc tgcagatcgg cgaccagttg ctggttttct tgtggacgca
    21001 gggaaacagg ttgaaaaacg ggcagacctt tgtcctcagc cagaacttta accgggctgg
    21061 gcatcagttt tttaccgcgt cctgccggtc ggtctggctg ggtaaacacg ccaacgatgt
    21121 tatgaccaga agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt gtacccgcaa
    21181 aaataatacg tagtgattct gacacgttaa ttcttatcct taagcccggg ctttcagacg
    21241 atccagtttt tcaactttct gacgaatacg ttgttgtttc agcggtgaca gataatccat
    21301 aaacagtttg ccgaccaggt gatccatctc atgctgaata cagatggcta acagaccgtc
    21361 tgcttccagt tcaaatggtt taccgtcgcg gtccagggcg cgaattttaa ctttctctgc
    21421 gcgtggcact aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa tgcctgtttc
    21481 gccgcttttt tctaaaagct ccggattgat taacaccagc cgttcgtcac ggttttccga
    21541 aacatcaatt acaatgatac gttgatggat atcaacctgg gttgccgcca ggccaatacc
    21601 ttcttctgcg tacatcgtct cgaacatatc atcgacgata cgctgaattt ctgcattcac
    21661 ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta acacttgcaa
    21721 aactgacata aatctccaga gatgtgttca ggagttagaa agattatttc ttctattcta
    21781 gacaaatccc cctctgattg acagcatcac tgaccaatcg caaagattgc taaggctgct
    21841 tatggcaggg agatagggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc
    21901 agcttgtatg gcgatgatat ggtccgtata gctcactggc tggcaaaaca gtcgcatatt
    21961 gatgcggttg tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca
    22021 tttccgcgaa agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta
    22081 atccctgcgg acagcgaatt ttatcctcct caacttctgg cgacgacaga ttaccccggc
    22141 gcactgtttg ttgaaggaga actgcacgcg ttgcattcat ttcagcttgc cgtagtgggg
    22201 agtcgggcgc attcatggta tggcgagcga tggggacgat tattttgcga aactctggcg
    22261 acgcgtggag tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggcgcataaa
    22321 gcggccttac aggtaaatgg cgttagcatt gctgtattgg ggaatggact taataccatt
    22381 catccccgcc gccatgctcc actggctgcc agtctacttg agcaaggtgg tgctctcgtc
    22441 tcggaatttc ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt
    22501 atcagtggcc taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg
    22561 gtgacagcac gttgtgcgct tgagcagggg cgtgaagttt ttgctttgcc aggtccaata
    22621 gggaatccgg gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg
    22681 gaaccggaag aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct
    22741 gaaaattcat tttattcacc agatcaggaa gacgtggcat tgccatttcc tgagctcctg
    22801 gctaacgtag gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct
    22861 gtgccagagg tagttactca actactcgaa ctggagttag caggatggat cgcagctgta
    22921 cccggcggct atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt
    22981 tgaaacctat attcacacag aagctgagtt gcgtgtggat caagacaaac ttgaacagga
    23041 tcttaccgac gcaggatttg atcgagaaga tatctacaat gccctgcttt ggctggaaaa
    23101 gcttgcggat tatcaggaag gactggcaga accgatgcaa ctggcctctg atccactctc
    23161 catgcgtatt tatacccctg aagagtgtga aaggctggat gccagctgtc gtggttttct
    23221 gcttttcctt gagcaaattc aggtgcttaa ccttgaaacc cgtgaaatgg tgatagagcg
    23281 agtgctcgcg ctggataccg cagagttcga cctggaagac ctgaaatggg taatcctgat
    23341 ggtgttgttc aatattccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt
    23401 tgaagtgaat gaaggtatgc tgcattaatc atttctttaa ttcagcataa gtagttatgg
    23461 cgaaatcagc actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac
    23521 tagttattcg atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg
    23581 actacgtccg tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc
    23641 aggtttgccc tgcatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta
    23701 ttggttgcag taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa
    23761 cagcaattac atgcccccaa tgccggacgg gccatctggt tcagcgccgc tcccgttatg
    23821 gcaaaacatt tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac
    23881 ccatagctgg agaatgccct gagtgccatt atccgctact catcgaaaag aaaaccgcgc
    23941 agggtgtaaa acacttttgt gccagtaaac aatgtggaaa gccgatttcg gcggaataat
    24001 aacgtgaata ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa
    24061 gaacgtgtca tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc
    24121 gaaacagcag tgatgagact gttggagtta aaacggcgtc cggttgataa ggggctgatt
    24181 ttaatcgcgg caaattgcga gcagcttaaa ccctatattg atgacaccat gctgactgac
    24241 gcgcagcgtg aaaccatttt ttcccgctgg ccaggtcctg tcacctttgt ctttcccgcg
    24301 cctgcgacaa caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc
    24361 gaccatccgt tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt
    24421 gccaacttga gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc
    24481 gctgcgttcc cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc
    24541 gatgccctga cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt
    24601 tggtaatccg atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact
    24661 gaatattgaa catccctatg ggcgtgtgtt ggcacccatc aatgatttca tcaacacact
    24721 gaacactttc tttagtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga
    24781 ggcttttgcc agagcggatg agcttactga acgggcagcg ttggctggtg ctgttaatac
    24841 cctcaagcgg ttagaagatg gacgcttgct gggtgacaat accgatggta taggtttgtt
    24901 aagcgatctg gaacgtctgt cttttatccg tcctggttta cgtcttctgc tcatcggcgc
    24961 tggtggcgca tctcgcggcg tactactgcc actcctttcc ctggactgtg cggtgacaat
    25021 cactaatcgg acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag
    25081 tattcacgcg ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc
    25141 aacatccagt ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg
    25201 catttattgc tatgatatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga
    25261 gcagcgaggc tcaaagcgta ctgctgatgg tttaggaatg ctggtggcac aagcggctca
    25321 tgccttcctt ctttggcatg gcgttctgcc tgatgtagaa ccggtcatca aactattgca
    25381 acaggagttg tcagcgtgaa tcaggccatc cagtttccgg atagggaaga gtgggacgag
    25441 aataaaaaat gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgtgcgatc
    25501 tctggcgaga gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt
    25561 tttcgtcagc atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaaat
    25621 gaagatgatc aaggttgggt gtggttaccc tgatccatat attcgtcctt ccatttcacg
    25681 tagttatttg cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt
    25741 ttgacggggc taccgagata cagatatccg ctctccagcc gtttattttg tgggaccagg
    25801 ctacctgcac caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc
    25861 atcccaacca aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca
    25921 gtgacatctt cgccaatggt taatgggttg ccggctgggt tgtacgagga tttatgagtg
    25981 acatgcaaca tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca
    26041 tctccacgaa tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca
    26101 atcacgacgc tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg
    26161 tatgggcgta aaacctcaga catactcacc tcaacaataa atgatttact aatgactttg
    26221 ggtacattat tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg
    26281 aatgcagcaa aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa agtacttgtg
    26341 caaaaaattg ggaaccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca
    26401 agatagtcgt aacaacaaag agaaaaatcc tgaaattcag ggttgactct gaaa
//
LOCUS       DAZKHK010000077        26291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKHK010000077 DAZKHK010000000
VERSION     DAZKHK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.077"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            268..939
                     /locus_tag="ACHOS5_003109"
     CDS             268..939
                     /locus_tag="ACHOS5_003109"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP9777883.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            949..2145
                     /locus_tag="ACHOS5_003110"
     CDS             949..2145
                     /locus_tag="ACHOS5_003110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9777884.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2180..2704
                     /locus_tag="ACHOS5_003111"
     CDS             2180..2704
                     /locus_tag="ACHOS5_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9777885.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGANCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPDGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2706..3479
                     /locus_tag="ACHOS5_003112"
     CDS             2706..3479
                     /locus_tag="ACHOS5_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP9777886.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHSNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3666..3941
                     /gene="frmR"
                     /locus_tag="ACHOS5_003113"
     CDS             3666..3941
                     /gene="frmR"
                     /locus_tag="ACHOS5_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP9777887.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3976..5085
                     /gene="frmA"
                     /locus_tag="ACHOS5_003114"
     CDS             3976..5085
                     /gene="frmA"
                     /locus_tag="ACHOS5_003114"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP9777888.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5178..6011
                     /gene="fghA"
                     /locus_tag="ACHOS5_003115"
     CDS             5178..6011
                     /gene="fghA"
                     /locus_tag="ACHOS5_003115"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9777889.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHIADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYASVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6137..6676)
                     /locus_tag="ACHOS5_003116"
     CDS             complement(6137..6676)
                     /locus_tag="ACHOS5_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP9777890.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDACSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            6743..6841
                     /locus_tag="ACHOS5_003117"
                     /pseudo
     CDS             6743..6841
                     /locus_tag="ACHOS5_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
     gene            6878..7960
                     /gene="lacI"
                     /locus_tag="ACHOS5_003118"
     CDS             6878..7960
                     /gene="lacI"
                     /locus_tag="ACHOS5_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP9777891.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQACHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPITEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            8083..11157
                     /gene="lacZ"
                     /locus_tag="ACHOS5_003119"
     CDS             8083..11157
                     /gene="lacZ"
                     /locus_tag="ACHOS5_003119"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP9777892.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRIIEVTSEYLFRHSDNELLHWSVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSGTDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKVAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVINVDVAVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            11209..12462
                     /gene="lacY"
                     /locus_tag="ACHOS5_003120"
     CDS             11209..12462
                     /gene="lacY"
                     /locus_tag="ACHOS5_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP9777893.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            12528..13139
                     /gene="lacA"
                     /locus_tag="ACHOS5_003121"
     CDS             12528..13139
                     /gene="lacA"
                     /locus_tag="ACHOS5_003121"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP9777894.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     DKRESLIKEMFATVGENAWVEPPVYFSYGSNIYIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVATGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(13260..14543)
                     /gene="codA"
                     /locus_tag="ACHOS5_003122"
     CDS             complement(13260..14543)
                     /gene="codA"
                     /locus_tag="ACHOS5_003122"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP9777895.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHFHDGKISAIDTQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITRHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(14533..15792)
                     /gene="codB"
                     /locus_tag="ACHOS5_003123"
     CDS             complement(14533..15792)
                     /gene="codB"
                     /locus_tag="ACHOS5_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9777896.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(16117..18003)
                     /gene="prpE"
                     /locus_tag="ACHOS5_003124"
     CDS             complement(16117..18003)
                     /gene="prpE"
                     /locus_tag="ACHOS5_003124"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP9777897.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPGALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHLQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(18043..19494)
                     /gene="prpD"
                     /locus_tag="ACHOS5_003125"
     CDS             complement(18043..19494)
                     /gene="prpD"
                     /locus_tag="ACHOS5_003125"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP9777898.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(19528..20697)
                     /gene="prpC"
                     /locus_tag="ACHOS5_003126"
     CDS             complement(19528..20697)
                     /gene="prpC"
                     /locus_tag="ACHOS5_003126"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP9777899.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEVQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(20742..21632)
                     /gene="prpB"
                     /locus_tag="ACHOS5_003127"
     CDS             complement(20742..21632)
                     /gene="prpB"
                     /locus_tag="ACHOS5_003127"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP9777900.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARNQAK"
     gene            21871..23457
                     /gene="prpR"
                     /locus_tag="ACHOS5_003128"
     CDS             21871..23457
                     /gene="prpR"
                     /locus_tag="ACHOS5_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP9777901.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLASERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKMSLAALSVPFSAALRQGLETCQIVLLLYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(23558..23833)
                     /gene="yahO"
                     /locus_tag="ACHOS5_003129"
     CDS             complement(23558..23833)
                     /gene="yahO"
                     /locus_tag="ACHOS5_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP9777902.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            23980..24654
                     /locus_tag="ACHOS5_003130"
     CDS             23980..24654
                     /locus_tag="ACHOS5_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP9777903.1"
                     /translation="MMKLVHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCGEIFSLIRIVGGAYLLWFAW
                     CSSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLHAETPT
                     WARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASWVIGAIIGVFALRLIY
                     EGVTQR"
     gene            complement(24671..24914)
                     /locus_tag="ACHOS5_003131"
                     /pseudo
     CDS             complement(24671..24914)
                     /locus_tag="ACHOS5_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25327..26142)
                     /gene="yahL"
                     /locus_tag="ACHOS5_003132"
     CDS             complement(25327..26142)
                     /gene="yahL"
                     /locus_tag="ACHOS5_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP9777904.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVRLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT         6240 a         6794 c         6733 g         6524 t
ORIGIN      
        1 tttttaatat gcaattaatt gacaaactaa atcacattta aatacaatat ataaaacaaa
       61 ataaccatac aaaccaaaca cacaaacact taacatatga actacataaa agattacaca
      121 ctcttatccc cgtacctgac agccccttgt ttttcagcta tcagctattt ttaacactta
      181 aaaattagca tctgtctgcg tcagaggcta cgtctctgcg ggcgtcgttg tgcttgtagt
      241 ataataaata aaatggagtg tcataaagtg gataaggttt tagattcagc gatcctttct
      301 tcggcaaata aaagaaaggg tatccttgcc attggcgcgc atcctgacga tatagaatta
      361 ggctgtggcg catcgctagc tcgccttgcg caaaaaggaa tttatattgc taccgtggtg
      421 atgactaccg gtaattctgg cgtagatgga ataatcgatc gccacgaaga atcacgcaac
      481 gctctaaaga tattagggtg ccaccaaact attcatctta attttgctga cacccgcgct
      541 catttacagc tcaatgatat gatttctgcc ctggaaaaca tcataaaaaa tcaaattccc
      601 tctgatgttg aaattatacg tgtatatacc atgcatgatg ctgaccgcca tcaggatcat
      661 cttgcagtct atcaagcctc aatggttgcc tgtcgcgcta ttccgcaaat tctcggctat
      721 gaaacaccaa gtacctggct ttcatttatg cctcaggttt ttgaatccgt gaaagaagaa
      781 tatttttctc ttaaactgac tgcgttaaaa aaacataaaa gtcagagcca gcgagattat
      841 atgcgcccgg agcgtctgcg tgcagttgct caatttcgcg ggcaacaggt caatagcgat
      901 ctgggtgaag gttttgttat tcataaaatg attctttgag caaattgcat gaaaacctgg
      961 atctttatat gtatggccgt agcaatattg ctatggtttc tgagtacgtt aaggcgtaag
     1021 cccagccaaa agaaaggctg tattgacgcc attatacctg cctataacga aggcccgtgt
     1081 ctggcgcagt cactggataa tctgctgcgt aacccttatt tttgccgggt aatttgcgtt
     1141 aacgacggct ccacggacaa taccgaagcg gtcatggcgg aagtcaaacg caaatggggc
     1201 gaccgctttg ttgccgtcac gcaaaaaaat accggtaaag gtggtgcgct gatgaatggc
     1261 ctcaattacg ccacctgcga tcaggttttt ttaagtgatg ccgacaccta tgttccgccc
     1321 gatcaagacg gaatgggcta tatgctggcg gaaattgagc gtggcgctga tgccgtaggc
     1381 ggcattccct ctactgcgtt gaaaggcgcg ggtctgttac cacacatccg cgcgaccgta
     1441 aagttgccga tgattgttat gaagcgcacg ctacagcagc tcctgggcgg cgcaccgttt
     1501 atcatcagcg gtgcctgcgg gatgttccgt actgatgtat tgcgtaagtt cggtttctcg
     1561 gatcgtacta aagtcgaaga ccttgatctc acctggacgt tggtggcaaa cggctaccgt
     1621 attcggcagg cgaatcgctg catcgtatac ccacaggaat gtaacagtcc gcgtgaggag
     1681 tggcgccgct ggcggcgttg gattgtgggc tacgcggtct gtatgcgcct gcataaaaga
     1741 cttttattta gccgcttcgg tatcttcagt atatttccta tgctgttggt tgtgctttat
     1801 ggcgttggta tttatctcac tacctggttt aatgaattca tcaccaccgg gccacatggt
     1861 gtggtgttgg caatgttccc gcttatctgg gtcggcgtag tttgtgttat tggtgcattt
     1921 agcgcctggt ttcatcgttg ctggttgttg gtgcctttag caccgctttc cgttgtgtat
     1981 gtattattag cttatgccat ctggattatt tatggactta ttgccttttt tactggacgc
     2041 gaacctcagc gcgacaaacc cacccgctat tccgcactgg tggaagcgtc aaccgcttat
     2101 tcccaccctt ctgtcacagg aactgaaaaa ctatctgaag cttaatgtta aagagaaaaa
     2161 tattcagatt gcagaccagg tgattattga tgaaagtgcc ggtgaggttg ttatcggcgc
     2221 gaatacccgt atttgccacg gtgccgttat tcagggtccg gtagtgattg gcgcaaactg
     2281 cctgataggt aattatgcgt ttattcgtcc tggcacaata atcagcaatg gcgtaaaaat
     2341 tggttttgcc accgaaatta aaaatgcggt tattgaagcg gaagcaacga ttggtccgca
     2401 atgctttatt gccgactcgg tagttgcaaa ccaggcatat ttgggcgcac aagtacgaac
     2461 cagtaatcat cgtctggatg aacagcccgt gtctgttcga actccagatg gaattatcgc
     2521 taccggatgc gataaattag gttgttatat cgggcagcgt tcacgccttg gcgtacaagt
     2581 tattattttg cctgggcgaa ttatttcccc gaacacgcaa cttggcccgc gcgtgattgt
     2641 agaacgtaat ttacctaccg gaacttattc actccgacaa gaacttatcc gtacaggaga
     2701 ttaaaatgat aaaacggacg ttattagcgg cggcaatttt tagcgcatta cccgtttatg
     2761 ccgggttaac ttccattacc gcgggctacg attttaccga ttattctggc gatcatagca
     2821 accgtaattt agcgtatgct gaactggtgg cgaaagttga aaacgcaacg ctgcttttta
     2881 atctttccca ggggcgtcgt gattatgaaa ctgaacattt caatgccact cgcggccagg
     2941 gtgcagtctg gtacaaatgg aataactggc tgacaacccg aacgggtatt gcctttgcgg
     3001 ataatacgcc ggtctttgcc cgccaggatt ttcgtcagga tattaacctg gccctgttgc
     3061 caaaaacgct tttcacgacc ggttatcgct acactaaata ttacgatgat gtcgaagtcg
     3121 atgcctggca aggtggcata tcactctata ctggcccggt gatcaccagc tatcgctata
     3181 cccattatga ctccagcgat gcaggtggta gttacagcaa tatgatttcc gtgcgtctga
     3241 atgacccgcg cggcactggt tacacgcaac tatggctaag ccgcggaaca ggtgcttaca
     3301 cctacgactg gacgccagaa acacgctacg gcagcatgaa gagcatcagt ttgcaacgta
     3361 ttcaaccgct gactgagcaa cttaatctcg ggttgacggc aggtaaagtg tggtacgaca
     3421 ccccaaccga tgattataac ggtctgcaac ttgcagccca tctgacctgg aaattctgat
     3481 tccttctgcc gcccgctatc cggggcggcc ttccctgccg attagccccc cccctttcct
     3541 ctttgttttc cgaccacatt caccggataa attttattct ccagtgttat atactatagg
     3601 ggggtatgca ttgacatata gcataccccc ctatagtata ttgcgtgcag atgatgaggt
     3661 gcgaaatgcc cagtactccg gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc
     3721 gggggcagat tgatgctctg gaacggtcgc tggagggtga tgccgaatgc cgtgccatac
     3781 tccaacagat cgctgccgtt cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa
     3841 gccatatccg ggaaacgttt gaccgaaatg actgctacag ccgcgaagtc agccaatccg
     3901 ttgacgacac tattgaactg gttcgtgcct atcttaaata gctgaatcta ttaccatatt
     3961 gaggaagagc gagagatgaa atcacgtgct gccgttgcat ttgctcccgg taaaccgctg
     4021 gaaatcgttg aaattgacgt tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc
     4081 cataccggtg tttgccatac cgacgcattt accctctccg gcgatgaccc ggaaggtgtc
     4141 ttcccggtgg ttctcggtca cgaaggggcc ggggttgtag ttgaagtcgg tgaaggggta
     4201 accagcgtca aacctggcga ccatgtgatc ccgctttaca ccgcagagtg cggcgagtgt
     4261 gagttctgtc gttctggcaa aactaacctg tgtgtcgcgg ttcgcgaaac gcagggcaaa
     4321 ggcttgatgc cagacggcac cacccgtttt tcttacaacg ggcagccgct ttatcactac
     4381 atgggatgct caacattcag tgaatacacc gtggtagcgg aagtgtctct ggccaaaatt
     4441 aatccagaag cgaaccatga acacgtctgc ctgctgggct gtggcgtgac caccggtatt
     4501 ggcgcggtac acaacacagc taaagtccag ccaggtgatt ctgttgccgt gtttggtctt
     4561 ggcgcgattg gtctggcagt ggttcagggc gcgcgtcagg cgaaagcggg acggattatc
     4621 gctatcgata ccaacccgaa gaaattcgaa ctggcgcgtc gcttcggtgc caccgactgc
     4681 attaacccga atgactacga caaaccaatt aaagatgttc tgctggatat caacaaatgg
     4741 ggtatcgacc atacctttga atgcatcggt aacgtcaacg tgatgcgtgc agcgctggaa
     4801 agcgcgcacc gcggctgggg tcagtcggtg atcatcgggg tagcaggatc tggtcaggaa
     4861 atctccaccc gtccattcca gttggtcacc ggtcgcgtat ggaaaggttc cgcgtttggc
     4921 ggcgtgaaag gtcgttccca gttaccgggt atggttgaag atgcgatgaa aggtgatatc
     4981 gatctggaac cgtttgtcac gcataccatg agcctggatg aaattaatga cgccttcgac
     5041 ctgatgcatg aaggtaaatc cattcgaacc gtaattcgtt actgatttcc cgctggttta
     5101 ccccgtccag ttcagacggg gttcttaata ctctccccgg gcagccgtcc gggggattaa
     5161 ccgtgagata atgactgatg gaactcattg aaaaacatgc cagctttggc ggctggcaaa
     5221 atgtgtatcg gcattattcc caatcactga aatgtgaaat gaatgtcggc gtctatctcc
     5281 caccaaaagc cgagaatgaa aaattgccgg tgttgtactg gctttcaggc ctgacctgca
     5341 acgagcagaa tttcattact aaatcgggga tgcagcgtta cgcggctgag cacaacatta
     5401 ttgttgttgc gccggacacc agtccgcgag gcagtcatat cgcagatgct gaccgttacg
     5461 atctcgggca aggtgccggg ttttacctga acgcgacgca agcgccgtgg aatgaacatt
     5521 acaaaatgta tgactatatc cgcaacgagc tgccgaattt agtgatgcat cattttccag
     5581 caacggccag aaagtctatc tcaggtcatt ctatgggcgg gctgggcgcg ctggtgctgg
     5641 cgttacgtaa cccagatgaa tatgccagcg tctcggcgtt ttcgcccatt gtctccccat
     5701 cgcaagtacc gtggggacag caagcctttg ccgcatatct tggtgaaaat aaagatgcct
     5761 ggttggatta cgacccggtg agtcttattt cacaaggtca acgcgttgcg gaaatcatgg
     5821 ttgatcaggg gttgagtgat gatttttacg cagaacaact gcggactcca aatcttgaaa
     5881 agatctgcca ggagatgaat atcaagacgt taatccgtta tcacgagggt tatgatcaca
     5941 gctattattt tgtctccagt tttattggcg agcatattgc gtaccacgcc aataaactga
     6001 atatgcgttg agcatctgcg cacggctgcc ggatgcggcg tgaacgcctt atccggccta
     6061 cacttcgccc gtaaaccgta ggcctgataa gacgcgtaag cgtcgcatca ggcatcactg
     6121 cacggcttat ctatgtttac cacatcaaat catcaggcac tttgaagtcg gcatacggat
     6181 cgtcttcatc ctgctcttcg gcgctgagcg cgctgtgcaa cacaatactg caggcatcac
     6241 gctgagcaat tttatcggca acgctggcgg gaataatggc gtattcacct tcgctattgt
     6301 tatcaaccaa caagcgggca atcgccagac gaccattaat caactgcgtt tgcgtgagct
     6361 tatcaacaaa aatcttctta atcagattgc cgtcagtgaa gttaaaacca atatcgccat
     6421 tggcaatggt aattcggttc atttcaatga gctgcttcac ctgggcttta tattctttcg
     6481 ccaacgccgc ttgtttttgt tgctcgctaa gctgtttatc acgctcaagc tgtgcctttt
     6541 tattttcttc taccgccgcc cgagcttcac gcgcctgaac acgcgatttt tttgccgttc
     6601 tctcgacctt cgccgctttt ttgctggtga ctagtcccgc tttgagcaac tgctcttgta
     6661 aggtaagttt tgccatcttt gtttccaaac ccgctgaata atttttagcg agtatacctg
     6721 taaacgccgt aggtgtattg gcgtgggttt tctctcacct gtgacctggc gtcagcattt
     6781 taaatctcac ttcggcgtca gcctcgccga atggcgcaaa acctttcgcg gtatggcatg
     6841 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     6901 tgtcgcggag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggcctg
     6961 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     7021 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     7081 ttccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     7141 tcaattgggt gccagcgtgg tggtgtcgat ggttgaacga agcggcgtcg aagcctgtaa
     7201 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     7261 ggatgaccag gatgccattg ctgtggaagc tgcctgcgct aatgttccgg cgttatttct
     7321 tgatgtctct gaccagactc ccatcaacag tattattttc tcccatgaag acggtacgcg
     7381 actgggcgtg gagcacctgg tcgcattggg tcaccagcaa atcgcgctgt tagcaggccc
     7441 attaagttct gtctcggcgc gtctgcgtct ggcaggctgg cataaatatc tcacacgcaa
     7501 tcaaattcag ccgataacag aacgggaagg cgactggagt gccatgtccg gttttcaaca
     7561 aaccatgcaa atgctaaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     7621 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     7681 ctcggtagtg ggatacgacg ataccgaaga cagctcgtgt tatatcccgc cgttaaccac
     7741 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     7801 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     7861 cacccttccg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7921 gctggcacga caagtttccc gactggaaag cgggcagtga gcgcaacaca attaatgtga
     7981 gtcagctcac tcattaggca ccccagtctt tacactctat gtgtccggct cgtatgttgt
     8041 gtgaaattgt gagcggataa caatttcaca caggatacag ctatgactat gattacggat
     8101 tctctggccg tcgtattaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8161 cgccttgcgg cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8221 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     8281 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgacgccga tactgtcgtc
     8341 gtcccctcaa actggcagat gcacggttac gatgcgccta tctacaccaa cgtgacctat
     8401 cccattacgg tcaatccgcc gtttgttccc gcggagaatc cgacaggttg ttactcgctc
     8461 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
     8521 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     8581 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
     8641 gtgatggtgc tgcgctggag tgacggcagt tatctagaag atcaggatat gtggcggatg
     8701 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ccacgcaaat cagcgatttc
     8761 caagttacca ctcgctttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     8821 atgtacggcg agctgcgcga tgaactgcgg gtgacggttt ctttgtggca gggtgaaacg
     8881 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggcggttat
     8941 gccgatcgcg tcacgctacg cctgaacgtt gaaaatccgg aactgtggag cgccgaaatc
     9001 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
     9061 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     9121 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     9181 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     9241 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
     9301 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     9361 ctgaccgatg atccgcgctg gctacccgcg atgagcgaac gcgtaacgcg gatggtgcag
     9421 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
     9481 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccttcccg cccggtacag
     9541 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     9601 gtggatgaag accagccctt cccggctgtg ccaaaatggt ccatcaaaaa atggctttcg
     9661 ctgcctggag aaatgcgccc gctgatcctt tgcgaatatg cccacgcgat gggtaacagt
     9721 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     9781 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
     9841 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
     9901 ctggtctttg ccgaccgcac gccgcatccg gcgctgacgg aagcaaaaca ccaacagcag
     9961 tatttccagt tccgtttgtc cgggcgaatc atcgaagtga ccagcgaata cctgttccgt
    10021 catagcgata acgaactcct gcactggtcg gtggcgctgg atggcaagcc gctggcaagc
    10081 ggtgaagtgc ctctggatgt tggcccgcaa ggtaagcagt tgattgaact gcctgaactg
    10141 ccgcagccgg agagcgccgg acaactctgg ctaacggtac gcgtagtgca accaaacgcg
    10201 accgcatggt cagaagccgg acacatcagc gcctggcagc aatggcgtct ggcggaaaac
    10261 ctcagcgtga cactcccctc cgcgtcccac gccatccctc aactgaccac cagcggaacg
    10321 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt
    10381 tcacagatgt ggattggcga tgaaaaacaa ctgctgaccc cgctgcgcga tcagttcacc
    10441 cgtgcgccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
    10501 tgggtcgaac gctggaaggt ggcgggccat taccaggccg aagcggcgtt gttgcagtgc
    10561 acggcagata cacttgccga cgcggtgctg attacgaccg cccacgcgtg gcagcatcag
    10621 gggaaaacct tatttatcag ccggaaaacc taccggattg atgggcacgg tgagatggtc
    10681 atcaatgtgg atgttgcggt ggcaagcgat acaccgcatc cggcgcggat tggcctgacc
    10741 tgccagctgg cgcaggtcgc agagcgggta aactggctcg gcctggggcc gcaagaaaac
    10801 tatcccgacc gccttactgc agcctgtttt gaccgctggg atctgccatt gtcagacatg
    10861 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
    10921 tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagccaacaa
    10981 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
    11041 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
    11101 gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
    11161 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
    11221 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
    11281 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
    11341 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
    11401 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
    11461 gtgatgtttg cgccgttctt tatttttatc ttcggaccac tgttacaata caacatttta
    11521 gtaggatcga ttgttggtgg tatttatctt ggcttttgtt ttaacgccgg tgcgccagca
    11581 gtagaggcat ttatcgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg
    11641 atgtttggct gtgttggctg ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc
    11701 aataatcagt ttgttttctg gctgggttct ggctgtgcac tcatcctcgc cgttttactc
    11761 tttttcgcca aaacggatgc gccctcttca gccacggttg ccaatgcggt aggtgccaac
    11821 cattcggcat ttagccttaa gctggcgctg gaactgttca gacagccaaa actgtggttt
    11881 ttgtcactgt atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct
    11941 aatttcttta cttcgttttt tgctaccggt gaacagggta cgcgggtatt tggctacgtc
    12001 acgacaatgg gcgaattact taatgcctcg attatgttct ttgcgcccct gatcattaat
    12061 cgcatcggtg ggaaaaatgc cctgctgctg gctggcacta ttatgtctgt acgtattatt
    12121 ggctcatcgt tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt
    12181 gaagtaccgt tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt
    12241 ttttcagcga cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt
    12301 atgtctgtac tggcgggcaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg
    12361 ctgggtctgg tggcgctggg cttcacctta atttccgtgt tcacgcttag cggccccggc
    12421 ccgctttctc tactgcgtcg tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc
    12481 ggcgcgagcg ccttatccga ccaacatatc agggcggaat gatcgcaatg aacatgtcga
    12541 tgacagaaag aataaaagca ggcaagctat ttaccgatat gtgcgaaggc ttacctgaaa
    12601 aaagacttcg tggaaaaacg ttaatgtatg agtttaatca ctcgcatcca tcagaagttg
    12661 ataaaagaga aagcctgatt aaagaaatgt ttgccacggt aggggaaaac gcctgggtag
    12721 aaccccccgt ctatttctct tacggttcca acatctatat aggccgcaat ttttatgcaa
    12781 atttcaattt aaccattgtc gacgactaca cggtaacgat cggtgataac gtactgattg
    12841 cacccaacgt tactctttcc gttacgggac acccggtaca ccatgaattg agaaaaaacg
    12901 gagagatgta ctcttttccg ataacgattg gcaataacgt ctggatcgga agtcatgtgg
    12961 ttattaatcc aggcgtcacc atcggggata attctgttat tggcgcgggt agtatcgtca
    13021 caaaagacat tccaccaaac gtcgtggcta ctggcgttcc ttgtcgggtt attcgcgaaa
    13081 taaacgaccg ggataagcaa tattatttca aagattttaa agttgagtct tcagtttaaa
    13141 taagcaaaat tacctgatgc gctccgctta tcaggcctac aatttcagtg atctacgtag
    13201 gccggataag gtgttcacgc cgcatccggc ataaactacg ctcgcggtag cccagccgat
    13261 taccgtttgt aatcgatggc ttctggctgc tccagatata cggtggtttg tgccggttgt
    13321 gtgctggcaa tcaccttacc gccacgtacc gaataacgta ccggaacctg acggcgcaac
    13381 gcatcaaacc cattttcagc cggcaggata atcaggttgg cgctgtttcc ggcggcaatg
    13441 ccgtaatcct gcaaattcaa cgtcctggcg ctatggcggg tgattaaatt caggccgtcg
    13501 ttaatctgcc cgtagcccat cagttggcaa acatgcagcc ccatatgcag cacttgcagc
    13561 atattcgccg ttcccagcgg ataccacgga tcgaagacat catcatgacc aaagcagacg
    13621 ttaatgccgg actccagcat ctctttaacg cgcgtgatgc cgcgacgttt tggataggta
    13681 tcgaaacgtc cttgcagatg gatattgacc agcgggttgg cgacaaagtt aataccggac
    13741 attttcagca agcggaacag gcgtgaggta tacgccccgt tataggagtg cattgccgtg
    13801 gtgtggctgg cggtgactcg cgcgcccatg ccttcacggt gcgccagggc agcaacggtt
    13861 tcaacaaagc gcgactgttc gtcatcaatc tcatcacagt gaacatcgat gagacggtcg
    13921 tatttttgcg ccagggcgaa ggttttatgc agcgactcca cgccatattc acgggtaaat
    13981 tcaaaatgcg gaatcgcccc aacaacgtct gcccctaagc gtaacgcctc ttccagcaac
    14041 gcttcaccat tgggatacga caaaatccct tcctgcggga aggcgacgat ttgcagatca
    14101 atccacggcg caacttcctg tttcacttcc agcattgctt tcagcgcagt cagcgttgcg
    14161 tccgaaacat cgacatgggt acgcacatgc tgaatgccgt tggcaatctg ccatttcagc
    14221 gtttgccatg cgcgttgttt cacatcgtca tgagttaata acgctttgcg ctcggcccag
    14281 cgttcaatgc cttcaaacag cgtgccggac tgattccagt tcggttgtcc ggcggtttgc
    14341 gtggtgtcca ggtgaatatg cggctccaca aacggcggta aaactaaacc ttgttcggca
    14401 tccaggctgt tttcagttac gggcatcaca ccagattgcg tatcaatggc gctgattttt
    14461 ccgtcgtgaa aatgaatctg ccacagcccc tctttgccgg gtaaccgggc gttaataatt
    14521 gtttgtaaag cgttattcga cactgttagc ctccacatgc gtcattgctg ctgtcgtttt
    14581 acgattcaaa atcgggttaa ggatcagata gctcagcgcg ccacctaata ccgcgttgac
    14641 cggaacaatt cccggtaacc agtggcctgc ggcaatcccc agcgcgaccg ccagaatcgc
    14701 cacccaattg acactcatca tacgcgtggt cgcaaagtgc tcatagcggc gacggttcat
    14761 cagataatca gcgatgatca cgccgcccac cggaggaata gctgccgaaa ggaaggtcaa
    14821 ccagccgaca aaattgttat acagccataa tgcgcagacc gtaccgataa taccgttaat
    14881 caccgaaagg gttttgctcg acataccggt aatgttggcg aaacctaaac ccgacgcata
    14941 gagcgcgtta tcgttggtgg tccagatatt cagccccagc accacaatcg caggcagcag
    15001 aaggccctga gcaatcatca catcagagat atccgccatc cccagtgccg ccgcgcccgc
    15061 tgcaccgaaa ataaacatca acgagttgcc gaggaaaaag gccaccatcg ccaccagcac
    15121 cgccagtttg gcattacgac caaaccggac aaagtcagcg gtgagcgtgc ccgcactgat
    15181 aaatgacccc acaaccagcg ccagcgcgac attgaaatct aacggttgtg cgggaacgac
    15241 cgcttttaat gcgtccaggc cgcccatgcc gttaacggcc aaccacacgg aataaccgcc
    15301 aagacaggcg atagccggaa ccgcaatcac cgaaagaact gtcagcgccg aaatgccaaa
    15361 gaagacggtg acggtcatca gtaaaccgga gacggcaatc agcaaattaa tatccagccc
    15421 ggttgccttg cccaccggaa tagcaaacat cgccacgcca acgccaaacc agccaacttg
    15481 ggtgccgccc agcagcagtg aaggcaacca tgagccttta acaccaaacg agaagcgagc
    15541 aagaagatgg gtggtcaggc cggtttttgc gccaatgtaa ccaagaaatg aagtgtaaat
    15601 accgaggaga agattaccga tgagaactgc gaggaagaaa tcatgatagc taagaccggt
    15661 tccgagagtg ccgccggtcc acatactggc ggaaaagaag gttaatccca gcatgacgaa
    15721 cgtcaatgcc aatacccctt tccgcgccga ctgcgggact ggcccctggc taaagttgtt
    15781 atcttgcgac acgaaattcc ccctttttgc tgtttgaaac ccaaaaaatc cgccgcattc
    15841 tattcatctg aagataaaaa gcaatcgttt tcgtaggggg atatattttt tatatgaatg
    15901 agtgcaaaaa ggcgggggaa acttatctgt gtaaaaacag tgggtaggca tgataagacg
    15961 cgtcagcgtc gcatcaggca tctgcgcatg gtgtcggatg cggcgcgagc gccttatccg
    16021 acctacggtt caggcctgta ggcatgataa gacgcgtcag cgtcgcatca ggcatctgcg
    16081 cacggtgtcg gatgcggcgc gagcgcctta tccgacctac tcttccatcg cctggcggat
    16141 ctgatccaac gacgccggat catcaatggt cgtcagatcc ccaggatcgc gtccttcgca
    16201 aatcgcctgg atcgtgcggc gcagcatttt tccggatcgc gtttttggca attgcgagac
    16261 aaaccagacg tgcgccgggc ggccaaagtt gccaatctgg ctgtctacca gcgccataat
    16321 tgccttctct tgcaagtgcg ccacctcacg gtcttccaga ctgtcgctct ctttcggaat
    16381 gacaaacgcc accgccacct gccctttcag cgcatctttc accccaacca ccgccacttc
    16441 ggcaacgccc ggatgactgg agatactctc ttcaatctca cgcgtaccca gccgatgtcc
    16501 ggcaacgtta atcacatcgt cagtgcgccc gagaataaag tgataaccgt cagcatcgcg
    16561 gatgccccag tcaaaagtgg cgtacaccgg acgggaaaac agcgaccagt acgtcttcac
    16621 aaagcggtcg tcgtcgcccc agatggtctg aatacagcct ggcggcaatg gcccctccac
    16681 taccagcatc cctttctcat tgacgccaca cggttcgccg gtgacttcat tgagcaactg
    16741 cacgttatag ccatacatcg gcacgccggg gcttcccaga cgcgtcggcc tgtcatccag
    16801 accgcgagca atcgccataa tcggccagcc ggattcggtc tgccagtagt tgtcgatgac
    16861 cggcacatcc agcgtattgc tcacccaact ggcggtcggc tcatccagcg gttctccagc
    16921 cagatagagc acttccagcg acgaaagatc gtgtttgcga atttcagcgg tagggaattt
    16981 tttcagcacg cgaatggcgg tcggcgctga gaacatgcgg ctaacctgat atttctcgac
    17041 aattttccac cacacgccgc agtccggcca ggtcggcaat ccttcgtaaa cgatagtcgc
    17101 catccccgcc agcagcggcg cgtaaacgat atacgaatgc cctaccaccc agccgatatc
    17161 cgaagcacaa aagaacacgc cgcccgcttt gccgccaaaa atggtgtcca tcgaggtcgc
    17221 cagcgccacc gcatatccgc cgacgtcacg ctgcacgcct ttaggtttac cggtcgtgcc
    17281 ggaagtgtag agaatgcagg aggtttcgtt ggattccagc catgccaccg gcacccgcgc
    17341 gccgatgtgt tgatggcgca acgacgcgaa atcgacatcc cgcccgctaa cgcgcgccat
    17401 tttcgccagc ccgcgatcca ccagtaaaac gtgacgcggc tgatgctgtg cctgacttat
    17461 cgcatcgtcg agcaattttt tatacggaat gattttaccg ccgcgcgccc cggcatcagc
    17521 cgagacaatc agcaccggtt tagcgtcatc aattcgcgcc gccacgctgt gcgaggcaaa
    17581 accaccaaac accaccgagt gaatagcgcc aatgcgcgcg caggccagca gggtaatatg
    17641 cgcttcggca atcatcggca tatacaccag cacccgatcg ccgcgctgca cacccagcga
    17701 gcgcagcatt gacgccaccg cgttcacttc gtcatgcagt tggcgaaagg taaaggtacg
    17761 ctcttcctct gtttccgaag agacggcaat caatgccagc gcccctggct gtttctccag
    17821 ccagcgatcg atagcgttgt gacacaagtt ggttcggcct tcacaaaacc aacgggcaaa
    17881 cggcgggttg ctgtggtcaa gcgtttgcgt aaagggcgtc tgccagtcaa tacgccgggc
    17941 ctgctcggcc cagaactgct ccggttcgtt aatcgaacgc tgataaaatt cgctaaaaga
    18001 cataatgctc tcctgttgaa cttacgcctt accgccgttt acttaaatga cgtacaggtc
    18061 gagatactca ttgaccggca tctgttccag gcgagttctg tcgagagaaa cctccagaat
    18121 gcgctgctgc tggcgagtcg ggaactggcg cgcgagattg attttgaatt tatcgaccag
    18181 cttcggaatg ccatcctgac ggcggcgagc atgaccaatg gggtactcca ccaccacttc
    18241 ttcaaagcgc gtgccgtcgg tgaactcaag ggttatggca ttggcgatgg cgcgtttttc
    18301 cgggtcgtgg tagtcagcgg taaacgccgg atcttcaaag caattgatct tctcgcgcag
    18361 ggcgtcgatg cgtttatctt gcgcaacgtt gtcctcgtaa tctgccgccg ttaagcgccc
    18421 gaacagcagc gggatcgcca ccatgtactg aatgcagtgg tcgcggtcag ccgggttatt
    18481 gagcggccct tttttgtcga tgatgcgaat acaggcttcg tgggtgcgaa tggtcacttt
    18541 ttcgatatcc gccgccgttt tgcctgctgc ctgcatctgt tcatagagcg tcatcgctgc
    18601 ttcaactgcc gtctgggagt ggaactccgc cgggaaggag attttgaaca gcacattttc
    18661 catcacgtaa gaaccatacg ggcgctggaa gcggaacgat tcgcctttaa aggagacgtc
    18721 gtagaagccc cacacaggcg cagtcagggc tgacggataa cccatttcgc cggttttcgc
    18781 catcagtgcc agacgtaccg cgcgggaagt agcatcgccc gccgcccagg atttacgcgt
    18841 gccggtgttc ggcgcatggc gataggtgcg cagcgactga ccgtccaccc acgccagtga
    18901 aacggcgttg agaatttcct cgcgggtcag gccgagcatt tcggcgacca cggcggtgga
    18961 agccactttc actaacagaa cgtggtcgag accaacgcgg ttaaaggagt tttccagcgc
    19021 gatgcagccc tgaatttcat gggctttgat cattccggtc agcacctgtt tcatggtcaa
    19081 cggcgctttg ccgctggcga ccgcgttgcg cgaaagccag tccgccgttg ccagaatgcc
    19141 gccgaggttg tcggaaggat gcccccactc cgccgccagc caggtatcgt tgaaatcgag
    19201 ccaacggatc atcgcgccga tgttaaatgc cgcctggacg gggtcgagct ggaactgagt
    19261 tccggggacg cgcacgccgt taggtacgac ggtgccgggg acaattggcc ccagcagttt
    19321 tttacaggct gggtattcga gagcttcaag accgcagccg agcgtgtcga gcaggcagta
    19381 atgtgcggtg tcgtaggcta ctttggagct gatttcgtag ttcatcacgt aatcgacgat
    19441 atcaacgatt tcacgatcaa attccgggcg gatgttgttg atttgagctg acattgggta
    19501 cgtttcctta ttgttattcg tagaggttta ctggcgctta tccagcgcga caaactggcg
    19561 gtcttccggt ccaacataat tggcggaagg acggataatt ttgttgtcct gacgttgttc
    19621 gataatgtgc gccgcccagc cggtgacgcg ggcgataaca aacagtgggg tgaacatctc
    19681 ggtgggaacg cccatcatgt tgtaggaaac agcggagaac cagtcgagat tggggaacat
    19741 ctttttgctc tcccacatca ccgtttccag gcgatcggcg atgttgtaca tcttcagcga
    19801 cccgccttcc tgcgagagct gcttcgccac acgtttgatc acctgatgac gcgggtcggc
    19861 gatggtataa accggatgac caaaaccaat gaccacttct ttgttttcca cccgcttgcg
    19921 gatatcggct tcggcttcgt ccggcgtttc gtagcgttgc tggacctcca gcgacacttc
    19981 attcgccccg ccgtgcttcg gcccgcgcag tgcaccaatc gcgccaataa tcgcggaata
    20041 catatcagag ccggtgcccg caatcacccg gctggtaaag gtggaggcgt taaactcgtg
    20101 ttcggcgtac agcaccagcg aaatatgcat cgccttttcc cagctttgcg acggcttttc
    20161 gccatgcagc agatgcagga agtgaccgcc gatggagtcg tcatcggttt ccggctgaat
    20221 gcgttcgccg ttgtggctgt agtgatacca gtagagaaga atcgagctaa gtgacgccag
    20281 cagtttgtcg gcaatatccc gcgcgccaga gacggtatgc ccctcttttt ctggcagcgt
    20341 gcagccgagc gcggaaacgc cggtgcgcat aacatccatc gggtgtgacg ccgccggtaa
    20401 ggcttccagc acggtacgca cgttagccgg taaaccgcgc agggctttca gtttcgtttt
    20461 gtaggcggcg agttcgtcac gggtcggcag tttgccgtgg atcagcagat gcgctacttc
    20521 ttcaaattcg caatgttccg ccagatcaag aatatcgtaa ccacggtaat gcaagtcatt
    20581 gccactttta cccacggtgc agagcgccgt attgcccgcc ggaacgccag aaagtgctac
    20641 cgattttttc ggtttaatga catgagtact gttttgcagg atcgttgtgt cgctcatatt
    20701 gtcctcgtca tttgtagggt cgagatattt ttcgttgtgt tttatttcgc ctggttacgg
    20761 gcaaacaggt cgtcgagctt ctcttcgtac tggtagtagt tgatgctttc gtacagctcg
    20821 ttgcgggtct gcatggtgtc gatgacgctt ttctgcgtgc cttcctgacg caggacgttg
    20881 tagacatgtt cagcggcgcg gttcatggca cggaacgctg aaagcgggta cagcgccatt
    20941 gcaacatggg cactgcgtaa ttcatcggta gtaaacagcg gcgtggcacc aaattcggta
    21001 atgttagcga ggatcggcac ctgcaccgca tcggcaaact ggcggtacat ggcgagttcg
    21061 gtaatcgcct ccgggaacaa catctcggca cccgcttcaa cataggcctg cgcacgctcg
    21121 atcgcggcgt ccagcccctc taccgccagc gcatcggtgc gcgccatgat cacaaaatca
    21181 ggatcggttt tcgcatccac cgccgcgcgg atccgatcca ccatctcctc tttcgagacg
    21241 atcgctttgt tcgggcgatg accgcagcgt ttcgcaccaa cctgatcttc aatatgcaat
    21301 cctgccgcac cggctttaat catcgatttc acggtgcgcg ccacgttaaa agcagaagaa
    21361 ccaaaaccga tatccgcatc caccagcagc ggcagcgaac aaacgtcggt gatacggcga
    21421 atatctgtca gcacgtcatc aagagtagaa ataccgagat cgggcagccc cagcgaccct
    21481 gccgccacgc caccgccgga gagataaatt gcctgatatc cggcacgctg cgccaacagc
    21541 gcatggttgg cgttaatggt gccaacaatc tgcaatggat tttctttaga cagtgcagcg
    21601 cgaaacgctt tacctggaga gtgtagagac atagcccatc ctttgttatc aacttgttat
    21661 ttgcgttgat aaagacaaag caaggggtgt gccacaattg actgaataaa attaaatgtt
    21721 taatttcaat ttgttagaga taaagtcatg aagtaatccg caaaaatgcg tttcagttaa
    21781 cgtttcaggc aatgtttcac cgcgtttcat tgcaacaatt atgaaacaag gctaaaccca
    21841 atattcggtt tcttaacttt gcggtgcgct atggcacatc caccacggct taatgacgac
    21901 aaaccggtta tctggacggt atcggtaacg cgcctgttcg agctgtttcg cgatatcagt
    21961 ctcgagtttg atcacctggc gaatatcacc cctattcagc ttggctttga aaaagcggtg
    22021 acctacatcc gcaagaaact ggcaagcgaa cgctgtgacg ccatcatcgc tgctggctct
    22081 aacggcgcgt atctgaaaag ccgcctgtca gtgccagtta ttttgattaa accgagcggc
    22141 tacgatgtgt tacaggcact ggcaaaagcc ggaaaactca cctcttctat cggcgttgtc
    22201 acttatcagg aaactattcc ggcactggtg gcgtttcaaa aaacctttaa tttgcgcctc
    22261 gatcaacgta gctacattac cgaggaagac gcacgcgggc agattaacga gctaaaagct
    22321 aacggcaccg aagcggtggt cggtgcgggg ctgattaccg atctggcaga agaagccgga
    22381 atgaccggaa tttttatcta ttccgccgcc accgtgcgcc aggcattcag cgatgcgctg
    22441 gatatgacgc gcatgtcgtt acgccataac actcacgatg ccacccgtaa cgccctgcgt
    22501 actcgttacg tgctgggcga tatgctcggt caatcaccac agatggaaca ggtgcggcag
    22561 actattttgc tgtatgcccg ctccagtgca gcggtgttga ttgaggggga aacggggacg
    22621 ggcaaagagc tggcggccca ggcgattcat cgggaatatt ttgcccgcca cgatgcgcga
    22681 cagggcaaaa agtcgcatcc gtttgttgcc gtcaactgcg gggcgattgc cgaatcgttg
    22741 ctggaagcag agctgtttgg ctatgaggaa ggggcgttca ccggctcgcg acgcggaggt
    22801 cgcgccgggc tgttcgaaat cgcccacggt ggcacgctgt ttctggatga gattggcgaa
    22861 atgccgctac ctttgcagac tcgcctgtta cgggtgctgg aagaaaaaga ggtcacccgc
    22921 gtcggcgggc atcagcctgt tccggtagat gtgcgggtca ttagcgccac tcactgcaat
    22981 ctggaagaag atatgcagca agggcagttt cgccgtgacc tgttttatcg gctgagtatt
    23041 ttacgcctgc aactgccgcc gctacgcgag cgggtggcgg atattctgcc gctggcggaa
    23101 agctttttga aaatgtctct ggcggcactc tctgtccctt tttctgccgc attacgtcag
    23161 ggattagaga cctgtcagat tgtattattg ctctacgact ggccgggcaa tattcgtgaa
    23221 ctgcgcaata tgatggaacg actggcgcta tttttaagtg tggaaccgac gccggattta
    23281 acgccgcaat ttttgcagct gctactgccg gaactggcgc gcgagtcggc gaagactccc
    23341 attccaggct tgctgacagc acaacaggca ctggagaaat ttaatggcga taagacagca
    23401 gcggcgaatt atttaggtat cagccggacg acgttctggc ggcggctgaa aagctgaact
    23461 gccgaatgca gcgcgaacgc tttatccggc ataaatttcg atagatgatg atcaccgata
    23521 aacttacgca tcggggatgt ttacatagga ttcagaatta cttcttctta taaatatttg
    23581 ccgtgccgtg gatcttattg tcagtttgac cagaggtcag taccaacaca tcagcccctt
    23641 tttcatcggc ttttttgatc aaatcttctt tcgcatctgc ggtcgacatt tcattactgg
    23701 ttgaaatatc acctattttt tcatattgcg atgcaacctt ttcaaattcc gctttggtca
    23761 ttaattctgc ggcatagaca ttggtaacgg caaacgctaa cgcaccgact aacattttag
    23821 agattatttt catattacgg ctccagagtt gttatcacac tatcacccac ccacattatt
    23881 gggttgatta ataattagtt cagtcagggc ataaacgcca gtaaccggaa ataagacact
    23941 ttgtgatctc tctcgcaccc gattattttt ggtagaaata tgatgaagtt agttcactta
    24001 tttatggatg aaatcactat ggatcctttg cacgccgttt acctgaccgt aggattgttc
    24061 gtgattactt tttttaatcc gggagccaat ctctttgtgg tagtacaaac cagcctggct
    24121 tccggtcgac gcgcaggggt gttgaccggg ctgggcgtgg cgctgggcga tgcattttat
    24181 tccgggttgg gtttgtttgg tcttgcaacg ctaattacgc agtgtgggga gattttttcg
    24241 cttatcagaa tcgtcggcgg cgcttatctc ttatggtttg cgtggtgtag tagcatgcgc
    24301 cgccagtcaa caccgcaaat gagcacacta caacaaccga ttagcgcacc ctggtatgtc
    24361 tttttccgcc gtggattaat taccgatctc tctaacccgc aaaccgtttt attttttatc
    24421 agtattttct cagtaacatt acatgccgaa acaccaacat gggcacgatt aatggcctgg
    24481 gcggggattg tgctcgcatc aattatctgg cgagtctttc ttagtcaggc gttttctttg
    24541 cccgcagtgc gtcgtgctta tgggcgtatg caacgcgttg ccagttgggt tattggtgca
    24601 attattggtg tattcgcgct acgcctgatt tacgaagggg tgacgcagcg gtgagcagat
    24661 aatttactct ctacgtcatc aacctgattt gccgaatccc aaccatcggc gatgatagcc
    24721 cccgtaatgc gtggtcctta tttccgataa gtttacttga gtctttagct tcccatttca
    24781 gccactctcc cctgtgtggg ctttttcatt ggtctgaccc gtcctgaata gccaaacaat
    24841 ggcaatgttc cactttgcag ggatgataat aaggggatct gattcagaaa agttttgaaa
    24901 agttggaccg ccatcgaggc ctcgaacccc gtaccataca acaatcctgg ttgtcgtctg
    24961 ctcttcctga tgagctaatg gcggtttttg atatgagcct tacagcatat tttctgtcag
    25021 gcttgagtgc gatgcgtaag atacagaaaa gaccccacac aaacatgatg gaattatgat
    25081 ttttttaata tgaatgtcaa cccacacatt cacaaaacat ctgcatgaga tcatttttta
    25141 tacaccgtat agctgcgaaa ccactgctga tgctttttta agcgaagtgt cgccgatcac
    25201 aacaaaatat cggcgaaaaa ggcggtggaa tgccatttgg catacggata tacagatgaa
    25261 atgatcacct gctttgcgat tcactctctg gtggaataac aactcgcagg tgaaaccctt
    25321 tctgaactaa agagtgtagc ggattttaac gtgcttattt acaggataat atctgtcacc
    25381 taattcctgc actctattcg atatccattc gtttaatcgc gcatcatctc tgtatgtttt
    25441 tacgtccaga acagagagca ataacctcac cagcgattca ggaaaaacag ggtagatatt
    25501 ttttaagtgt aataataatc catgcgcaag cgcctgctta atgggcgcgt cggtctccgg
    25561 gatagacgac atttcgcgaa gccatttttc cacatgcata cactctttcc ctttagggaa
    25621 aagtctcagc aaaggcatat ggtgttcaag aagaatacat ttgcaatctg ttcgcactga
    25681 atcgataaac tgcactaatt tcttaagccc ggtaaagcgt ccagcctgca catttgcttt
    25741 aatatcaaga aacgcatcat cctggtcata tacccaaact gtcccttttt gattttcaag
    25801 tatgcgaaaa acaaacttaa ccgggtgatc agggtaatcc atccagtcat aaaatccagg
    25861 gaaatcatta aactgcacat ttatggtgag tgcgttatcg tctgaccact cacagtctcg
    25921 cacggctgcc ataagatgaa tgagtttgtc atcatcgact aaatcatcac gacaatcata
    25981 aggataatca taatattgtt taatcaaaac cttgaatata tcgacacagg aaaccagaga
    26041 gcttttgata tcctccggta actggttgtt tttaaccgta ttaagtatgc tttgttgcgt
    26101 atatggcatc aaattattgt gcggagcttt taatgatatc atcgcgatct ctcttaaata
    26161 aaaataaacg tcgcagataa taaagccctg attaaaccag gcactatcag aaatcgctca
    26221 taactaacga gtgaagtatt gtgtactgga gggagttata ttaacaattt gaatttacgt
    26281 tataggtttt a
//
LOCUS       DAZKHK010000078        25968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKHK010000078 DAZKHK010000000
VERSION     DAZKHK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.078"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            304..1443
                     /gene="wza"
                     /locus_tag="ACHOS5_003133"
     CDS             304..1443
                     /gene="wza"
                     /locus_tag="ACHOS5_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP9777905.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1449..1892
                     /gene="wzb"
                     /locus_tag="ACHOS5_003134"
     CDS             1449..1892
                     /gene="wzb"
                     /locus_tag="ACHOS5_003134"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP9777906.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFTAVYTLLERSARQWAQALNAEQV"
     gene            1895..4057
                     /gene="wzc"
                     /locus_tag="ACHOS5_003135"
     CDS             1895..4057
                     /gene="wzc"
                     /locus_tag="ACHOS5_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP9777907.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRNRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4150..4989
                     /gene="wcaA"
                     /locus_tag="ACHOS5_003136"
     CDS             4150..4989
                     /gene="wcaA"
                     /locus_tag="ACHOS5_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP9777908.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            4992..5480
                     /gene="wcaB"
                     /locus_tag="ACHOS5_003137"
     CDS             4992..5480
                     /gene="wcaB"
                     /locus_tag="ACHOS5_003137"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP9777909.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRAAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVVGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5477..6694
                     /gene="wcaC"
                     /locus_tag="ACHOS5_003138"
     CDS             5477..6694
                     /gene="wcaC"
                     /locus_tag="ACHOS5_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP9777910.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNNLYGPGRCRIIN
                     NGIDMATEAILADLPPVCETQGKPKIAVVAHDLRYDGKTKQQLVREMMALSDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6669..7886
                     /gene="wcaD"
                     /locus_tag="ACHOS5_003139"
     CDS             6669..7886
                     /gene="wcaD"
                     /locus_tag="ACHOS5_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP9777911.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            7897..8643
                     /gene="wcaE"
                     /locus_tag="ACHOS5_003140"
     CDS             7897..8643
                     /gene="wcaE"
                     /locus_tag="ACHOS5_003140"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP9777912.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLKGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8659..9207
                     /gene="wcaF"
                     /locus_tag="ACHOS5_003141"
     CDS             8659..9207
                     /gene="wcaF"
                     /locus_tag="ACHOS5_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP9777913.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            9234..10355
                     /gene="gmd"
                     /locus_tag="ACHOS5_003142"
     CDS             9234..10355
                     /gene="gmd"
                     /locus_tag="ACHOS5_003142"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9777914.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10358..11323
                     /gene="fcl"
                     /locus_tag="ACHOS5_003143"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10358..11323
                     /gene="fcl"
                     /locus_tag="ACHOS5_003143"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP9777915.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11326..11805
                     /gene="gmm"
                     /locus_tag="ACHOS5_003144"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11326..11805
                     /gene="gmm"
                     /locus_tag="ACHOS5_003144"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9777916.1"
                     /translation="MFLRQEDFSTVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTEFTTHY
                     VVLGFRIRVAEDELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLSEKRAGVPGL"
     gene            11802..13025
                     /gene="wcaI"
                     /locus_tag="ACHOS5_003145"
     CDS             11802..13025
                     /gene="wcaI"
                     /locus_tag="ACHOS5_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP9777917.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAASSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13028..14464
                     /gene="cpsB"
                     /locus_tag="ACHOS5_003146"
     CDS             13028..14464
                     /gene="cpsB"
                     /locus_tag="ACHOS5_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP9777918.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14657..16027
                     /gene="cpsG"
                     /locus_tag="ACHOS5_003147"
                     /gene_synonym="manB"
     CDS             14657..16027
                     /gene="cpsG"
                     /locus_tag="ACHOS5_003147"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9777919.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVKRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFNALGAPVEFIKVHNMPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16082..17476
                     /gene="wcaJ"
                     /locus_tag="ACHOS5_003148"
     CDS             16082..17476
                     /gene="wcaJ"
                     /locus_tag="ACHOS5_003148"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP9777920.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17478..18956
                     /gene="wzxC"
                     /locus_tag="ACHOS5_003149"
     CDS             17478..18956
                     /gene="wzxC"
                     /locus_tag="ACHOS5_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP9777921.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDTI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGILLA
                     VQIAAGVLAFVVMIVLSRHSLVVEVKRQFCRSEKMKMLLRAG"
     gene            19617..20897
                     /gene="wcaK"
                     /locus_tag="ACHOS5_003150"
     CDS             19617..20897
                     /gene="wcaK"
                     /locus_tag="ACHOS5_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP9777922.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILSPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKKHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSMLERIGEAK"
     gene            20894..22114
                     /gene="wcaL"
                     /locus_tag="ACHOS5_003151"
     CDS             20894..22114
                     /gene="wcaL"
                     /locus_tag="ACHOS5_003151"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP9777923.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22125..23519
                     /gene="wcaM"
                     /locus_tag="ACHOS5_003152"
     CDS             22125..23519
                     /gene="wcaM"
                     /locus_tag="ACHOS5_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP9777924.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGASITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQTVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPVFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIAMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23677..24672
                     /locus_tag="ACHOS5_003153"
     CDS             23677..24672
                     /locus_tag="ACHOS5_003153"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP9777925.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPKITQ
                     IGDVRDQQVLDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPHENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24915..25808
                     /gene="galF"
                     /locus_tag="ACHOS5_003154"
     CDS             24915..25808
                     /gene="galF"
                     /locus_tag="ACHOS5_003154"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP9777926.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGVKFRKGIEKLLSE"
BASE COUNT         6365 a         6477 c         7087 g         6039 t
ORIGIN      
        1 caccttatat tcggaagtta atttctgcaa taaccctttc tcatcaacga ctgtatgcag
       61 agagggtttg aaaaattaaa ctaaatcagt gtattggtag ctaaaaagcc aggggcggta
      121 gcgtgtctgg atgcctgaaa gaccagtctg aattattctg tcaatagcct gcggatgaaa
      181 tcaatttttt taggactgat gccagttaaa tttttattca attaactgca aggtaaatgt
      241 gcatggcgac attgctatta atagtgcaca ggataattac tctgccaaag tgataaataa
      301 acaatgatga aatccaaaat gaaattgatg ccattattgg tgtcagtaac cttgataagc
      361 ggttgcacag tacttccggg cagcaatatg tcgacgatgg ggaaagacgt catcaaacag
      421 caggacgctg atttcgacct cgacaaaatg gtgaatgttt atccgttaac cccgcgcctg
      481 attgatcaat tacgcccacg acccaacgta gcgcgcccca atatgacgct ggaaagtgag
      541 atcgcgaatt accagtatcg cgtcgggccg ggggacgttc ttaatgtcac cgtctgggat
      601 cacccggaac tcaccacgcc agccggtcag taccgcagct ccagcgacac cggcaactgg
      661 gtacagcctg acggcaccat gttctacccg tatatcggca aggttcacgt cgtggggaaa
      721 acgctcgctg aaatccgcag tgatattacc gggcgcttag cgacgtacat cgctgacccg
      781 caggtggacg ttaatatcgc cgccttccgc tcgcaaaaag cctatatctc cggtcaggtg
      841 aataaatccg gtcaacaggc cattaccaac gtgcctctga ccattctcga cgctatcaac
      901 gccgcaggtg gcctgaccga caccgctgac tggcgcaacg tggtgctaac acacaatggt
      961 cgtgaagagc gcatttcttt gcaggcgctg atgcaaaacg gcgacctcaa tcagaaccga
     1021 ctgctttacc ccggcgatat tctttacgtg ccacgtaatg atgatctgaa agtgtttgtg
     1081 atgggtgaag tgaagaaaca gagcaccctg aaaatggact ttagcggcat gaccctgact
     1141 gaagccctgg gcaatgctga aggcatcgac atgaccacct ccaacgccag cggcatcttt
     1201 gtcattcgtc cgctgaaagg cgagagcggg cgtaacggca agattgccaa tatctaccag
     1261 ctggatatgt ccgatgcgac gtcgctggta atggcgacag aattccgcct gcaaccttat
     1321 gacgtggtgt atgtcaccac cgccccggta tcccgctgga accgtctgat caatcagttg
     1381 ctgccaacta ttagcggtgt ccgttacatg acggatacag ccagcgacat tcataactgg
     1441 taatcgtcat gtttaacaac atcttagttg tctgtgtcgg caatatttgc cgttccccga
     1501 cggcggaacg cttactgcaa cgttatcacc cggagctaaa agtggagtcc gctggactcg
     1561 gcgcgctggt cggtaagggc gctgatccta ccgctatcag cgtcgccgca gaacatcaac
     1621 tgtccctgga aggtcattgc gcacggcaaa tcagccgccg tctgtgtcgc aactacgacc
     1681 tgattctgac catggaaaag cgccatatcg aacgcttatg cgagatggca ccagagatgc
     1741 gcggcaaagt gatgctgttt ggtcactggg ataacgaatg tgaaatcccc gatccgtatc
     1801 gcaaaagccg ggaaacgttt acagcggtgt acacattact tgaacggtct gcccgccagt
     1861 gggcgcaggc attgaacgca gagcaggtat aagaatgaca gaaaaagtaa aacaacatgc
     1921 cgctccggta acgggcagtg atgaaatcga tattggtcgc ctggtcggca ccgtcattga
     1981 agcacgctgg tgggtgattg gcattaccgc cgtattcgcc ctctgtgccg tggtttacac
     2041 cttcttcgct acgccgattt atagcgccga cgcactggta caaatcgagc aaagcagcgg
     2101 caattcgctg gtgcaggata tcggatcggc attagccaac aaaccgcctg catcggacgc
     2161 cgagatccag ttgattcgtt cgcgcctggt gcttggtaaa acggtggacg atctcgacct
     2221 tgatattgcg gtgagcaaaa acacgttccc gattttcggt gcgggctggg agcgcctgat
     2281 gggacgccag aacgagacgg tgaaagtgac cacctttaac cgaccgaagg agatggcgga
     2341 tcaggtgttt acgcttaatg tgctggacaa caaaaactac accctgagca gcgatggcgg
     2401 ctttagcgcc cgtgggcaag cgggccagat gctgaaaaaa gaaggcgtca cgctgatggt
     2461 tgaagccatt cacgccagcc cgggcagtga gtttaccgtc accaaatact ccacgctggg
     2521 gatgatcaac caactgcaaa acagcctgac ggtaacggag aacggcaaag acgcaggcgt
     2581 tttgagcctg acttataccg gtgaagatcg cgaacagatc cgcgacattc ttaacagcat
     2641 cgcccgtaac tatcaggaac aaaatattga gcgcaaatcg gcggaagcgt cgaaaagcct
     2701 cgctttcctc gcgcaacagt taccggaagt acgtaaccgc cttgatgttg ccgaaaacaa
     2761 actgaatgcc ttccgtcagg ataaagattc agttgatctg ccgctggaag cgaaagcggt
     2821 gctcgattcg atggtgaaca tcgacgcgca gttgaacgaa cttaccttta aagaagcgga
     2881 aatctccaag ctgtacacca aagttcaccc ggcgtaccgc acgctgctgg agaaacggca
     2941 ggcgctggaa gacgaaaaag ccaaacttaa tggtcgcgta acggcgatgc cgaaaaccca
     3001 gcaggaaatt gtgcgtctga cccgcgatgt cgagtctggt cagcaggtct atatgcagct
     3061 gctgaataaa gagcaggagc tgaaaatcac cgaagccagc accgtcggcg atgtgcgcat
     3121 tgttgacccg gcaatcaccc agccaggtgt actgaaaccg aagaaggggc tgattatcct
     3181 cggggcgatt atccttggcc tgatgctctc tatcgtgggt gtgctgctgc gctcgttgtt
     3241 taatcgcggt atcgaaagcc cgcaggtgct ggaagaacac ggtatcagcg tctatgccag
     3301 catcccgctg tcggagtggc agaaagcgcg cgatagcgtc aaaaccatca aagggattaa
     3361 acgctataaa cagagccagc tactggcggt ggggaatcca accgatctgg cgattgaagc
     3421 catccgcagt ctgcgtacca gtctgcactt cgcgatgatg caggcacaga acaatgtgtt
     3481 gatgatgacc ggggttagcc cgtcaatcgg taaaaccttt gtctgcgcca acctggcggc
     3541 ggtgattagc cagaccaata aacgggtgtt gttgatcgac tgcgatatgc gcaaaggcta
     3601 cacccacgag ctgttgggca ctaataacgt taatggcctg tcggaaattc tgattggaca
     3661 gggcgatatt actaccgctg ctaaaccgac ctctattgcc aaatttgacc tgatcccgcg
     3721 cggtcaggta ccgccaaatc cttctgaact gttgatgagc gaacgctttg ccgaactggt
     3781 gaactgggcg agtaaaaact acgacctggt gttgattgat acgccgccga ttctggcagt
     3841 gaccgatgcg gcaattgttg gtcgtcatgt cggaaccacg ttaatggtgg cgcgttatgc
     3901 ggtcaacaca ttgaaagaag tggaaaccag tctgagccgc tttgagcaaa acggtattcc
     3961 ggtgaaaggg gtgattctga actccatctt ccgccgcgcc agcgcatatc aggattatgg
     4021 ctattacgaa tacgaatata agtcggatgc gaaataacga cgcctgcatt cgcaccgccc
     4081 cgtaggccgg ataaggcgtt cacgccgcat ccggcaagca aaccagctca taagccggga
     4141 gaacaaccta tgaaagacaa tccgctgatc tcaatctata tgccgacctg gaatcgccaa
     4201 caactggcga ttcgggcgat aaaatcggtc ctgcgccagg actataacaa ctgggagatg
     4261 atcatcgtgg atgattgctc cacctcctgg gaacaactac aacagtacgt caccgccctc
     4321 aacgatccgc gtatcactta cattcacaac gacattaaca gcggggcgtg cgcggtacgt
     4381 aaccaggcga ttatgctggc gcaaggggaa tacatcaccg ggatcgatga cgacgatgaa
     4441 tggataccca accgcctgag cgtcttcctc gcccataaac agcaactggt cacacacgcc
     4501 tttttgtacg ctaacgacta cgtttgccag ggcgaagtct attcccaacc ggcaagcttg
     4561 ccgctgtatc ccaaatcgcc gtattcacgc cgcctgttct acaaacgcaa tatcattggc
     4621 aatcaggtct ttacctgggc gtggcgtttc aaagagtgct tgttcgatac cgaactgaaa
     4681 gcggcacagg attacgacat cttcctgcgg atggtggtgg agtacggcga accgtggaaa
     4741 gtggaagagg cgacgcagat cctgcatatc aatcacggcg aaatgcagat cacctcgtca
     4801 ccgaaaaaat tctccggata cttccacttt taccgcaagc acaaagacaa attcgaccgc
     4861 gccagcaaaa aatatcagct gtttaccctc taccagatcc gcaataaacg catgacctgg
     4921 cgcactttac tgacgctgct gtcggtgcgt aacggcaaac gtctggctga tggtattcgg
     4981 gggcgctaaa gatgctggaa gatctgcgcg ccaacagctg gagtttacgc ccgtgctgca
     5041 tggttcttgc ctatcgtgcc gctcattttt gttcggtgtg gcgcaaaaag aacgtcctca
     5101 acaatctttg ggcggccccg ctgctggtgc tgtatcgcat tatcaccgaa tgctttttcg
     5161 gttatgaaat ccaggccgcc gcgaccattg gccgacgctt tactatccat cacggttacg
     5221 ccgtggtgat caataaaaac gtggtcgtgg gggatgattt caccattcgc cacggcgtca
     5281 ctatcggcaa tcgtggtgcc gacaacatgg catgtccaca cattggcaac ggcgtcgaac
     5341 tcggtgccaa cgtcattatt cttggtgata tcacgcttgg gaacaacgtc accgtgggcg
     5401 cgggcagcgt ggtgctcgac tcggtcccgg acaacgcgct ggtggtggga gaaaaagcgc
     5461 gagtgaaggt aattaaatga atattctgca atttaatgtg cgactggcgg aaggcggggc
     5521 ggcaggtgtg gcgttagatc tccaccagcg tgcgctgcaa caggggctgg catcacattt
     5581 tgtctacggc tacggcaaag gcggcaaaga gagcgtcagc catcagaact atccgcaggt
     5641 catcaaacat acgccgcgga tgaccgcgat ggcgaacatt gcgttgtttc gtctgtttaa
     5701 tcgcgatctg ttcggcaatt tcaatgagtt atatcgcacc attactcgca caccgggtcc
     5761 ggtggtcctg cattttcatg tgctgcacag ctactggttg aatcttaaga gcgtggtgcg
     5821 cttttgcgaa aaagtgaaaa accataaacc ggatatcact ctggtctgga cgttacacga
     5881 tcactggagc gttaccggac gctgcgcctt taccgacggt tgcgaaggtt ggaaaacggg
     5941 ctgccagaaa tgcccgacct taaataatta tccgccggtg aagattgatc gcgcacacca
     6001 actggtggcg ggcaaacggc agttattccg cgagatgctg gcactgggct gtcagtttat
     6061 ttcccccagc cagcatgtgg ctgacgcttt caataacctg tacggtccag ggcgttgccg
     6121 gattatcaat aatggcattg atatggcaac cgaagcgatt ctggcggact tgcctccggt
     6181 ttgcgaaacc cagggcaagc cgaaaatcgc agtggtggcg catgatctgc gttacgacgg
     6241 caaaactaag cagcaactgg tgcgcgagat gatggcgctg agcgacaaaa tcgaactgca
     6301 tactttcggt aagttctcgc cgttcaccgc tggtaacgtt gttaatcacg gctttgaaac
     6361 tgacaagcgc aagttgatga gcgcgctcaa ccagatggat gcgctggtgt tcagttctcg
     6421 cgtcgataac tacccgctga ttttgtgtga ggcgttatcg attggcgtgc cggtgattgc
     6481 cacccatagc gacgcggcgc gggaagtgct gcaaaaatcc ggcggtaaaa ccgtcagcga
     6541 agaagaggtg ctgcaactgg tgcagttaag caaaccggaa atcgcacaag cgatatttgg
     6601 taccacgctg gctgagttca gccagcgcag ccgcgccgcc tacagtggac aacagatgct
     6661 ggaggagtat gtcaacttct atcagaatct gtagctacct gctgctgccg ctgatctacc
     6721 tgctggttaa cgtcaaaatc gcccagcttg gcgaaagttt ccccatcacc atcgtcactt
     6781 ttttacctgt cttgttactg ctgtttttag agcgcatcag cgttaaaaaa ttgatgattg
     6841 ccttagggat tggcgcgggg ctcacggcgt ttaactacct gtttggtcag tcgttggatg
     6901 ccggtaaata cgtcacttca accatgctgt ttgtctatat tgtgatcatt attggcatgg
     6961 tgtggagtat tcgctttaaa acgatttcgc cccacaacca ccgcaagata ttacgctttt
     7021 tttatctggt ggtcgggttg gtggtggcgc tggcggcggt ggagatggcg caaattatcc
     7081 tcaccggtgg cagcagtatt atggagtcga tttcgaaata tctgatttac agcaacagct
     7141 atgtgctgaa tttcatcaaa ttcggcggca agcgcacgac agcactttat ttcgaaccgg
     7201 catttttcgc tctggcatta atctcaattt ggctcagcat caaacagttt ggtatcaaaa
     7261 cccctaaaac cgatgctatg attctcgcag ggataatatt atccggatcg ttttcagggg
     7321 ttatgacctt tatcctgttt tatttgctgg agtgggcatt tcaatatctg aataaagagg
     7381 cgattaagaa aaagttaccg ttagcattga tttctctggc tgtattcctg gttggtgtgg
     7441 taattgcatt tccttatatt tccacccgtc tgggcgattt aggaacggaa ggatcgtcat
     7501 cgtattatcg tattgtcggt ccgctggtga tggtcggtta ttctttgacc catattgacg
     7561 gtgtagtcag atttggctca ctttatgaat atgtcgcatc attcggaata tttaacggtg
     7621 cggatgtcgg aaaaaccata gacaatggtt tgtatctgct gattatttat ttttcctggt
     7681 tcgcggtgtt tttatcactg tggtacatgg ggaaagtgat aaaaatgatg atcaacgctt
     7741 ttggtgataa ccgcaatttt cgcgtgcaat tatatctttt tactccggta tcgctgtttt
     7801 ttaccggttc gatatttagc ccggaatatg catttttaat cgtctgtccg tttattttgc
     7861 gaaaagcgtt aaatattacg aggtaagaat aagaacatgt tgcttagcat aatcactgtc
     7921 gcgtttcgta acctcgcagg gatagtcaaa acacatgcct cgctggcgca tctggcgcag
     7981 gcggacgata tcagcttcga atggattgtt gtcgatggcg gttccaacga cggtacccgt
     8041 gagtacctgg aaaatctcaa gggtatctat aacctacgct ttgtcagcga gccagataac
     8101 ggtatctacg acgccatgaa caagggcatt gcgatggcac aaggcaagtt cgcgttgttt
     8161 ctcaactcgg gcgatatttt tcatcaggat gccgcaaatt ttgtccgtaa gttaaaagtg
     8221 caaaaagata acgtgatgat caccggcgat gcgctgctgg attttggcga cgggcataag
     8281 attaaacgta gcgccaaacc ggggtggtat atttatcaca gcctgcccgc cagtcatcag
     8341 gcgatatttt ttccggtatc cggcttgaaa aaatggcgtt atgacctgga atataaagtt
     8401 tcttccgact acgcgctggc agccaaaatg tataaagccg gttatgcatt taaaaaactc
     8461 aatggcctgg tgtctgaatt ttctatgggt ggagtatcta ccaccaataa tatggaatta
     8521 tgtgctgacg ccaaaaaagt ccaacggcag atattacatg tgcctggctt ctgggctgaa
     8581 ttatcctggc atttacgcca acgtacgacc tcaaagacga aagccttata taacaaaagc
     8641 tgaatataag ggaaaacgat gcaggattta agtggattct cggtgccgaa agggttccgg
     8701 ggtggcaacg ctattaaagt gcaattgtgg tgggcagtac aggcaacaat atttgcctgg
     8761 tcgccacaag tattgtatcg ctggcgggca tttttattac gtttattcgg cgcaaaaata
     8821 ggaaaaaacg tagtcattcg tccgtcagta aaaattacct atccgtggaa attaacgtta
     8881 ggtgattacg cgtgggtcgg cgatgaggtc aatttatata ccctcggtga aataaccatt
     8941 ggcgcacatt cggtgatatc gcaaaaaagt tatttatgca ctggtagcca cgaccatgca
     9001 agtcaacatt tcaccattaa cgccacgcct attgtgattg gcgagaaatg ctggctggca
     9061 accgatgtct ttgttgcccc tggcgtcacg atcggcgacg gcaccgtcgt gggtgcgcga
     9121 agcagtgttt ttaaatcgct tccggcaaat gtggtttgcc gggggaaccc cgcagtggtg
     9181 atacgcaaac gcgttgaaac tgaataaatt caaaaaatac agaggaataa gacatgtcaa
     9241 aagtcgctct catcaccggt gtaaccggac aagacggttc ttacctggca gagtttctgc
     9301 tggaaaaagg ttacgaggtg catggtatta agcgtcgtgc atcgtcattc aacaccgagc
     9361 gcgtggatca catttatcag gatccgcaca cctgcaaccc gaaattccat ctgcattatg
     9421 gcgacctgag tgatacctcc aacctgacgc gcattttgcg tgaagtgcag ccggatgaag
     9481 tgtacaacct gggcgcaatg agccacgttg cggtttcttt tgagtcaccg gaatataccg
     9541 cagacgtcga tgcgatgggt acgctgcgcc tgctcgaggc gatccgcttc ctcggtctgg
     9601 aaaagaaaac ccgtttctat caggcttcta cttctgaact gtacggtctg gtgcaggaaa
     9661 ttccgcagaa agagaccacg ccgttctacc cgcgatctcc gtatgcggtt gccaaactgt
     9721 acgcctactg gatcaccgtt aactaccgcg aatcctacgg catgtacgcc tgtaacggta
     9781 ttctcttcaa ccatgaatcc ccgcgccgtg gcgagacttt cgttacccgc aaaatcaccc
     9841 gcgcaatcgc caacatcgcc caggggctgg agtcgtgcct gtatctcggc aatatggatt
     9901 ccctgcgtga ctggggccat gccaaagact acgtaaaaat gcagtggatg atgctgcaac
     9961 aggaacaacc ggaagatttc gttattgcca ccggcgttca gtactccgtg cgtcagttcg
    10021 tggaaatggc ggcggcacag ctgggtatca aactgcgctt tgaaggcacg ggtgttgaag
    10081 agaagggcat tgtggtttcc gtcaccgggc atgacgcgcc gggcgttaaa ccgggtgatg
    10141 tgattatcgc cgttgacccg cgctacttcc gtccggctga agttgaaacg ctgctcggtg
    10201 acccgaccaa agcgcacgaa aaactgggct ggaaaccgga aatcaccctc agagagatgg
    10261 tgtctgaaat ggtggctaat gaccttgaag cggcgaaaaa acactctctg ctgaaatctc
    10321 acggctacga cgtggcgatc gcgctggagt cataagcatg agtaaacaac gcatttttat
    10381 cgctggccat cgcgggatgg tcggttccgc catcaggcgg cagctcgaac agcgcggtga
    10441 tgtggagctg gtattacgca cccgcgacga gctgaacctg ctggacagcc gcgcggtgca
    10501 tgatttcttt gccagcgaaa gcattgacca ggtctatctg gcggcggcga aagtgggcgg
    10561 tattgttgcc aacaacacgt atccggcaga tttcatctac cagaacatga tgattgagag
    10621 caacatcatt cacgccgcgc atcagaacga cgtgaacaaa ctgctgtttc tcggatcgtc
    10681 ctgcatctac ccgaaactgg caaaacagcc gatggcagaa agcgagttgt tgcagggcac
    10741 gctggagccg accaacgagc cttatgccat tgccaaaatc gccgggatca aactgtgtga
    10801 atcttacaac cgccagtacg gacgggatta ccgctcagtc atgccaacca acctgtacgg
    10861 gccacacgac aacttccacc cgagtaattc gcatgtgatc ccagcattgc tgcgtcgctt
    10921 ccacgaggcg acggcacaga atgcgccgga cgtggtggtg tggggcagcg gtacgccgat
    10981 gcgcgaattt ctgcacgttg atgatatggc agcggcgagc attcatgtca tggagctggc
    11041 gcacgaagtc tggctggaaa acacccagcc gatgttgtcg cacattaacg tcggcacggg
    11101 cgttgactgc actatccgcg agctggcgca aaccatcgcc aaagtggttg gttacaaagg
    11161 tcgggtggtt tttgatgcca gtaaaccgga tggtacgccg cgcaaactgc tggatgtgac
    11221 gcggttgcat cagcttggct ggtatcacga aatctcactg gaagcggggc ttgccagcac
    11281 ttaccagtgg ttccttgaga atcaagaccg ctttcggggg taatgatgtt tttacgtcag
    11341 gaagactttt ccacggtagt gcgctccaca ccgcttgtct ctctcgactt tattgttgag
    11401 aacagtcgcg gcgagtttct gcttggcaaa agaaccaacc gcccggcgca gggttactgg
    11461 tttgtgccgg gagggcgcgt gcagaaagac gaaacgctgg aagccgcatt tgagcggctg
    11521 acgatggcgg aactggggct gcgtctgccg ataacagcag gccagtttta cggtgtctgg
    11581 cagcactttt atgacgataa cttctccggc acggaattca ccactcacta tgtggtgctc
    11641 ggttttcgca tcagagtcgc ggaagatgag ctgttactgc cggatgagca gcatgacgat
    11701 taccgctggc tgacgccgga cgcgttgctc gccagtgaca atgttcacgc taacagccgc
    11761 gcctattttc tctctgagaa gcgtgccgga gtacccggat tatgaaaata ctggtctacg
    11821 gcattaacta ctcgccggag ttaaccggca tcggcaaata caccggcgag atggtggaat
    11881 ggctggcggc acaaggtcat gaggtgcggg ttattaccgc accgccttac tacccgcagt
    11941 ggcaggtggg cgagaactat tccgcctggc ggtacaaacg ggaagagggg gccgccacgg
    12001 tgtggcgctg cccgctgtac gtgccaaaac agccgagcac cctgaaacgc ctgttgcatc
    12061 tcggcagttt tgccgccagc agtttctttc cgctgatggc gcaacgtcgc tggaagccgg
    12121 atcgcattat cggcgtggtg ccaacgctgt tttgcacgcc aggaatgcgc ctgctggcga
    12181 aactgtctgg tgcgcgcacg gtgctccata ttcaggatta cgaagtggac gccatgctgg
    12241 ggctgggcct tgccggaaaa ggtaaaggcg gcaaagtggc acagctggca acggcgttcg
    12301 aacgtagcgg actgcataac gtcgataacg tctccacgat ttcgcgctcg atgatgaata
    12361 aagccatcga aaaaggcgtg gcggcggaaa acgtcatctt cttccccaat tggtcggaaa
    12421 tcgcccgttt tcaacatgtt gcagacgccg atgttgacgc ccttcgtaac cagcttggcc
    12481 tgccggataa caaaaaaatc attctttact ccggcaatat tggtgaaaag cagggactgg
    12541 aaaacgttat tgaagctgcc gatcgcctgc gcgatgaacc gctgattttt gccattgtcg
    12601 ggcagggcgg cggcaaagcg cggctggaaa aaatggcgca gcagcgtgga ctgcgcaaca
    12661 tgcaattttt tccgctgcaa tcgtatgacg ctttacccgc actgctgaag atgggggatt
    12721 gccatctggt ggtgcaaaaa cgcggcgcgg cagatgccgt attgccgtcg aaactgacca
    12781 atattctggc ggtaggcggt aacgcggtga ttaccgcaga agcccacacc gaactggggc
    12841 agctttgcga aacctttccg ggcattgcgg tttgcgtaga gccggaatcg gtcgaggcgc
    12901 tggtggcggg gatccgtcag gcgctcctgc tgcccaaaca caacacggtg gcacgtgaat
    12961 atgccgaacg cacgctcgat aaagagaacg tgttacgtca atttataaat gatattcggg
    13021 gataattatg gcgcagtcga aactctatcc agttgtgatg gcaggtggct ccggtagccg
    13081 cttatggccg ctttcccgcg tactttatcc caagcagttt ttatgcctga aaggcgatct
    13141 caccatgctg caaaccacca tctgccgcct gaacggcgtg gagtgcgaaa gcccggtggt
    13201 gatttgcaat gagcagcacc gctttattgt cgcggaacag ctgcgtcaac tgaacaaact
    13261 caccgagaac attattctcg aaccggcagg gcgtaacact gcacctgcca ttgcgctggc
    13321 ggcgctggcg gcaaaacgtc atagcccgga gagcgacccg ttaatgctgg tattggcggc
    13381 ggatcatgtg attgccgacg aagatgcgtt ccgcgccgcc gtgcgtaacg ccatgccgta
    13441 tgctgaagcg ggcaagctgg tgaccttcgg cattgtgccg gatctacctg aaaccggata
    13501 tggctatatt cgtcgcggtg aagtgtctgc gggtgagcag gatacggtgg cctttgaagt
    13561 ggcgcagttt gtcgaaaaac cgaatctgga aaccgctcag gcctatgtgg caagcggcga
    13621 atattactgg aacagcggta tgttcctgtt ccgtgccgga cgctatctcg aagaactgaa
    13681 aaaatatcgc ccggatattc tcgatgcctg tgaaaaagcg atgagcgccg tcgatccgga
    13741 tctcgatttt attcgcgtgg atgaagaagc gtttctggcc tgcccggaag agtcggtaga
    13801 ttacgcggtc atggaacgca cggcagatgc cgttgtggtg ccaatggatg cgggctggag
    13861 cgatgtcggt tcctggtctt cattatggga gatcagcgct cacaccgccg agggcaacgt
    13921 ttgccatggc gatgtgatta atcacaaaac tgaaaacagc tatgtgtatg ccgaatctgg
    13981 cctggtcacc accgtcgggg tgaaagattt ggtggtagtg cagaccaaag atgcagtgct
    14041 gattgccgac cgtaacgcgg tgcaggatgt gaaaaaagtg gtcgagcaga tcaaagctga
    14101 tggtcgccat gagcatcggg tgcatcgcga agtgtatcgt ccgtggggca aatatgactc
    14161 tatcgacgcg ggcgaccgct accaggtgaa acgcatcacc gtgaagccgg gcgaagggtt
    14221 gtcggtacag atgcatcatc accgcgcgga acactgggtg gttgtcgcgg gaacggcaaa
    14281 agtcactatc gacggtgata tcaaactgct tggtgaaaac gagtccattt atattccgct
    14341 gggggcgacg cattgcctgg aaaacccggg gaaaattccg ctcgatttaa ttgaagtgcg
    14401 ctccggctct tatctcgaag aggatgatgt ggtgcgcttc gcggatcgct acggacgggt
    14461 gtaaacgtcg catcaggcgc tgtttgtcgg atgcggcgtg aacgccttat ccgacctacg
    14521 gttcgggttt gtaggcctga taagacgcgg cagcgtcgca tcaggcaatg aatgcgaaac
    14581 cgcgatgtaa ataacgacaa aaacaaaatt ggccgtttcg gtcagggcca actattgcct
    14641 gaaaaagggt aacgacatga aaaaattaac ctgctttaaa gcctacgata ttcgcgggaa
    14701 attaggcgaa gaactgaatg aagatattgc ctggcgtatt gggcgtgcct atggcgaatt
    14761 tctcaaaccg aaaaccattg tgttaggcgg tgatgtccgc ctcaccagcg aaaccttaaa
    14821 actggcgctg gcgaaaggtt tacaggatgc gggcgtcgat gtgctggata ttggcatgtc
    14881 cggcaccgaa gagatctatt ttgccacgtt ccatctcggc gtggatggcg gcatcgaagt
    14941 taccgccagc cataacccga tggattacaa cggcatgaag ctggtgcgcg agggggctcg
    15001 cccgatcagc ggtgataccg gactgcgcga cgtcaagcgt ctggcagaag ccaacgactt
    15061 cccccccgtc gatgaaacaa aacgcggtcg ctatcagcaa atcaatctgc gtgacgctta
    15121 cgttgatcac ctgttcggtt atatcaacgt caaaaacctc acgccgctca agctggtgat
    15181 taactccggg aatggcgcgg cgggtccggt ggtggacgcc atcgaagccc gctttaacgc
    15241 cctcggcgca ccggtggaat tcatcaaagt gcacaacatg ccggacggca atttccccaa
    15301 cggtattcct aacccgctgc tgccggaatg ccgcgacgac acccgtaatg cggtcatcaa
    15361 acacggcgcg gatatgggca ttgcctttga tggcgatttt gaccgctgtt tcctgtttga
    15421 cgaaaaaggg cagtttatcg agggctacta cattgtcggt ctgctggcag aagcgttcct
    15481 cgaaaaaaat cccggcgcga agatcatcca cgatccgcgt ctctcctgga acaccgttga
    15541 tgtggtgacg gccgcgggcg gcacgccggt gatgtcgaaa accggacacg cctttattaa
    15601 agaacgtatg cgcaaggaag acgccatcta cggtggcgaa atgagcgccc accattactt
    15661 ccgtgatttc gcttactgcg acagcggcat gatcccgtgg ctgctggtcg ccgaactggt
    15721 gtgcctgaaa gggaaaacgc tgggcgaact ggtgcgcgac cggatggcag cgtttccggc
    15781 aagcggtgag atcaacagca aactggcgca ccccgttgag gcgattaacc gcgtcgaaca
    15841 gcattttagc cgcgaggcgc tggcggtgga tcgcaccgat ggcatcagca tgacctttgc
    15901 cgactggcgc tttaacctgc gcacctccaa caccgaaccg gtggtgcgcc tgaatgtgga
    15961 atcgcgcggt gacgtgccgc tgatggaagc gcgaacgcga actctgctga cgttgctgaa
    16021 cgagtaatgt cggatcttcc cttaccccac tgcgggtaag gggctaataa caggaacaac
    16081 gatgacaaat ctaaaaaagc gcgagcgagc gaaaaccaat gcatcgttaa tctctatggt
    16141 gcaacgcttt tcagatatca ccatcatgtt tgccggacta tggctggttt gcgaagtcag
    16201 cggactgtca ttcctctaca tgcacctgtt ggtggcgctg attacgctgg tggtgttcca
    16261 gatgctgggc ggcatcaccg atttttatcg ctcatggcgc ggtgttcggg cagcgacaga
    16321 atttgccctg ctgctacaaa actggacctt aagcgtgatt ttcagcgccg ggctggtggc
    16381 gttcaacaat gatttcgaca cgcaactgaa aatctggctg gcgtggtatg ggctgaccag
    16441 tatcggactg gtggtttgcc gttcgtgtat tcgcattggg gcgggctggc tgcgtaatca
    16501 tggctataac aagcgcatgg tcgcggtggc gggggattta gccgccggac aaatgctgat
    16561 ggagagcttc cgtaatcagc cgtggttagg gtttgaagtg gtgggcgttt accacgaccc
    16621 gaaaccgggt ggcgtttcta acgactgggc gggcaatctg caacagctgg tcgaggatgc
    16681 gaaagcgggc aagattcata acgtctatat cgcgatgcaa atgtgcgacg gcgcacgcgt
    16741 gaaaaaactg gtccatcaat tggcggacac cacctgttcg gtgctgctga tccccgatgt
    16801 ctttaccttc aacattctcc attcccgtat cgaagagatg aacggcgtac cggtggtgcc
    16861 gctgtatgac acgccgcttt ccggggttaa ccgcctgctc aaacgtgcgg aagatattgt
    16921 gctggcgacg cttatcctgc tgctgatctc cccggtgctg tgctgcattg cgctggcggt
    16981 gaaactgagc tcaccggggc cggttatttt ccgccagact cgctacggca tggacggcaa
    17041 gccgatcaaa gtgtggaagt tccgttccat gaaagtgatg gagaacgaca aagtggtgac
    17101 ccaggcgacg cagaacgatc cgcgcgtcac caaagtgggg aactttctgc gccgcacctc
    17161 gctggatgaa ttgccgcagt ttatcaatgt gctgaccggc gggatgtcga ttgtcgggcc
    17221 acgtccgcac gcggtggcgc ataacgaaca gtatcgccag ctcattgaag gctacatgct
    17281 gcgccataaa gtgaaaccgg gcattaccgg ctgggcgcag attaacggct ggcgcggcga
    17341 aaccgacaca ctggagaaaa tggaaaaacg cgtcgagttc gaccttgagt acatccgtga
    17401 atggagcgtc tggttcgata tcaaaatcgt tttcctgacg gtattcaagg gcttcgttaa
    17461 caaagcggca tattgatatg aacttacgtg aaaaaaccat cagcggcgcg aagtggtcgg
    17521 cgattgccac ggtgatcatc atcggcctcg ggctggtgca gatgaccgtg ctggcgcgga
    17581 ttatcgacaa ccaccagttc ggtttgctta ccgtgtcgct ggtgatcatc gcgctggcag
    17641 atacgctttc tgacttcggc atcgctaact cgattattca gcgaaaagaa attagccacc
    17701 ttgaactcac cacgctgtac tggctgaacg tcgggctggg gatcgtggtg tgcgtggcgg
    17761 tgtttttgtt gagtgatacc attggcgacg tgctgaataa cccggacctg gcaccgttga
    17821 ttaaaacatt gtcgctggcg tttgtggtga tcccccacgg gcagcagttc cgcgcgttga
    17881 tgcagaagga gctggagttc aacaaaatcg gcatgatcga aaccagtgcg gtgctggcgg
    17941 gcttcacttt tacggtggtt agcgcccatt tctggccgct ggcgatgacc gcgatcctcg
    18001 gttatctggt caatagtgcg gtaagaacgc tgctgtttgg ctactttggc cgcaaaattt
    18061 atcgccccgg tctgcatttc tcgctggcgt cggtggcacc gaacttacgt tttggtgcct
    18121 ggctgacggc ggacagcatc atcaactatc tcaataccaa cctttcaaca ctcgtgctgg
    18181 cgcgtattct cggcgcgggc gtggcagggg gatacaacct ggcgtacaac gtggccgttg
    18241 tgccgccgat gaaactgaac cccatcatca cccgcgtgtt gtttccggca tttgccaaaa
    18301 ttcaggacga caccgaaaag ctgcgcgtca acttctacaa gctgctgtcg gtggtgggga
    18361 ttatcaactt tccggcgctg ctggggctaa tggtggtgtc gaataacttt gtaccgctgg
    18421 tatttggcga gaagtggaac agcattattc cggtgctaca gctgctgtgt gtggtggggc
    18481 tactgcgctc cgtgggtaac ccgattggtt cgctgctgat ggcgaaagcg cgggtcgata
    18541 tcagctttaa attcaacgta ttcaaaacat ttctgtttat tccggcgatt gttattggtg
    18601 ggcaaatggc gggcgcaatc ggcgtcacgc ttggcttcct gctggtgcag attatcaaca
    18661 ccattctgag ctatttcgtg atgattaaac cggtgctcgg ttccagttat cgccagtaca
    18721 tcctgagttt gtggctgccg ttttatctct cgctgccgac gctggtggtc agttatgcgc
    18781 tgggcattgt gctgaaaggg caactggcgc tggggatact gctggcggtg caaatagccg
    18841 caggcgtgct ggcgtttgtg gtgatgattg tgctgtcgcg ccattcgctg gtggtggaag
    18901 tgaagcgtca gttttgtcgc agcgaaaaaa tgaaaatgct tttacgggcg gggtgaatgg
    18961 ttgttttcgg ttccggcaac ggttgccgga tgcggcgtaa aacgccttat ccgtcctggg
    19021 gggctgggct tggatcttgt aggcctgata agacgcgcca gcgtcgcatc aggcactgaa
    19081 tgccggatgc ggcgtgaacg ccttatccgt cctacggctc tgtgctcggg tcttgtaggc
    19141 ctgataagag gcgtcagcgt cgcatcaggc actgaatgcc ggatgcggcg taaaacgcct
    19201 tatccggcct acggttctgt gctcggatct tgtaggcctg ataagacgcg tcagcgtcgc
    19261 atcaggcact gaatgccgga tgcggcgtga acgccttatc cgtcctacgg ttctgtgctc
    19321 ggatcttgta ggcctgataa gacgcgtcag cgtcgcatca ggcactgaat gccggatgcg
    19381 gcgtgaacgc cttatccgtc ctacggttct gggcttggat cttgtaggcc tgataagacg
    19441 cgtcagcgtc gcatcaggca ctgaatgccg gatgcggcgt gaacgcctta tccggcctac
    19501 ggttctgtgc tcggatcttg taggtctgat aagacgcgtt agcgtcgcat caggcaaaaa
    19561 acggcaacga acacaacaaa cacagcctca cctggcatca aatttatgag gaaaaaatga
    19621 aattacttat tctgggcaac cacacttgcg gcaaccgcgg cgacagcgcc atcttgcgcg
    19681 gcttacttga tgccatcaat atcctcagtc cacatgccga agtggacgtg atgagccgct
    19741 atccggtcag ttcttcctgg ctgctcaacc gcccggtaat gggcgacccg ctgttcctgc
    19801 aaatgaaaaa acacaacagc gcggcgggcg ttgttgggcg cgttaaaaaa gtcctccgtc
    19861 gccgctacca gcaccaggta ttgctctcac gcgtcaccga cactggcaag ctgcgtaata
    19921 tcgccatcgc ccagggattc actgacttcg tgcgactgct gtcaggttac gacgccatta
    19981 ttcaggtcgg cggatcgttt tttgtcgatc tctacggcgt gccgcagttt gaacatgcgc
    20041 tttgcacttt catggcgaaa aagccgctgt ttatgattgg tcacagcgtc ggccccttcc
    20101 aggatgagca atttaaccaa ctggcgaact atgtctttgg tcactgcgac gcgctgatcc
    20161 tgcgcgaatc ggtcagcctt gatttgatga aacgcagcaa tatcaccacc gcaaaagttg
    20221 aacatggcgt cgataccgcg tggctggtcg atcaccacac agaagacttc accgccagct
    20281 atgccgttca acactggctg gacgttgccg cacaacagaa aacggtggcc attaccctgc
    20341 gcgaactggc accgttcgac aaacgtctcg gcaccactca gcaagcgtat gaaaaagcct
    20401 ttgccggggt ggtcaatcgc attctcgacg aagggtatca ggtgattgcg ctctccacct
    20461 gtacgggcat cgacagctat aacaaagacg accgcatggt ggcgctcaat ttgcgccagc
    20521 acatcagcga tcctgcccgt taccacgtgg tgatggatga actcaacgat ctggaaatgg
    20581 gcaaaattct gggggcctgt gaactcaccg ttggtacgcg cctgcactct gccattatct
    20641 cgatgaactt tgccactccg gcgattgcca tcaactacga acataaatcc gccgggatca
    20701 tgcaacaact ggggctacca gaaatggcga ttgatatccg tcatttatta gacggcagcc
    20761 tgcaagcgat ggttgcggat accttaggcc agcttccggc actgaacacg cgacttaacg
    20821 aagccgtcag tcgcgaacgt cagacgggaa tgcagatggt gaaatctatg cttgaacgca
    20881 tcggggaggc gaaatgaagg tcggcttctt tttactgaaa tttccgttgt cgtcggaaac
    20941 cttcgtcctc aatcaaatta ccgcgtttat tgatatggga tttgaggtgg agattgtcgc
    21001 gctgcaaaaa ggcgacacac aaaacaccca tgcggcatgg acgaaataca accttgccgc
    21061 cagaacccgc tggttacagg acgaacctac gggcaaagtg gcgaaactgc gccaccgcgc
    21121 cagccagacc ttacgcggca ttcatcgtaa aaatacttgg caggcgctca acctcaaacg
    21181 ctatggcgcc gagtcgcgga acctgatttt gtctgccatt tgcggtcagg tcgcaacacc
    21241 gtttcatgcc gatgtcttta tcgctcactt tggtccggca ggggtaaccg cagcaaaact
    21301 ccgggaactg ggtgtcattc gcggcaaaat tgccaccatt ttccacggca ttgatatttc
    21361 cagtcgggaa gtgctcaacc actacactcc tgaatatcag caactgttcc gccgtggcga
    21421 cctgatgcta ccgatcagcg atctgtgggc cggaaggctg caaaaaatgg gctgcccgag
    21481 ggaaaaaatt gccgtatcgc gcatgggcgt ggacatgacg cgctttagcc cgcgtccggt
    21541 gaaagcgccc gcaacgccgc tggaaatcat ttccgtcgca cgtttaaccg agaaaaaagg
    21601 cctgcatgtg gcgattgaag cctgtcggca gttgaaagag cagggtatgg catttcgcta
    21661 tcgcatcctc ggcattggcc cgtgggaacg acgcctgcgc accctcatcg aacaatatca
    21721 actggaagat gtggtagaga tgccgggctt taaaccgagc cacgaagtga aagcgatgct
    21781 agacgacgcg gatgtcttcc tgttgccatc ggttacaggt gcggatggcg atatggaagg
    21841 tattccggtg gcgctgatgg aagcgatggc ggtcggcatt ccggtggttt ctactctgca
    21901 tagcggaata cctgaactgg tggaggccga taaatccggc tggctggtgc ctgagaacga
    21961 tgctcgcgca ctggcgcaac gactggcggc gtttagccaa ctggacaccg acgaactggc
    22021 tccggttgtc aaacgtgcgc gcgaaaaagt cgaacacgat tttaaccagc aggtgatcaa
    22081 tcgagaactc gccagcttgc tgcaggcttt atagaggaga acgtatgcca tttaaaaaac
    22141 tctcccgacg caccttcctg acggcaagct ccgcgcttgc cttccttcat acgcctttcg
    22201 cccgtgcact tcctgcccga caaagcgtta acattaacga ctacaatcca cacgactgga
    22261 ttgcttcatt taaacaagcc ttcagcgaag ggcaaacagt cgtcgtgcct gccggattgg
    22321 tttgtgacaa tatcaacacc ggcatcttca ttcctcctgg caaaacgtta cacatccttg
    22381 gaagcctgcg cggcaacggc agagggcgtt ttgtcttaca ggatggcagc caggtgacag
    22441 gggaggaggg cggcagtatg cataacatca ccctggatgt gcgtggctct gactgcacca
    22501 tcaaagggct ggcgatgagc ggctttggcc cggtgacgca gatctatatc ggcggcaaaa
    22561 acaaacgggt catgcgcaac ctgaccatcg ataacctgac cgttagccac gctaattacg
    22621 ccattttgcg ccagggattt cataaccaga ttatcggtgc cagcatcacc aactgtaagt
    22681 tcagcgactt acagggcgac gccattgaat ggaacgtggc aattaacgac agtgatattt
    22741 tgatatctga ccatgtcatc gagcgcatca actgtactaa cggcaaaatc aactggggca
    22801 tcggcatagg ccttgcggga agtacttatg ataataacta cccggaagac cagacagtga
    22861 aaaactttgt cgtggcgaat atcacgggat cggattgtcg gcagttgatc catgttgaaa
    22921 atggtaaaca ttttgttatt cgtaatatca aagcccgcaa tatcacgccg gttttcagta
    22981 agaaagcagg cattgataac gccacagtcg ctatttacgg ttgtgacaat ttcgtgattg
    23041 ataatattga aatgattaat agtgccggga tgttaatcgg ctatggggta attaaaggca
    23101 aatatctctc gataccgcag aatttccggg tgaataatat tcaactggat aacacccacc
    23161 ttgcttataa attgcgcggc atccaaatct ctgccgggaa tgctgtttcc tttgtggcac
    23221 tgactaacat tgcgatgaag cgtgcgtcat tggagctgca caacaagccg caacaccttt
    23281 ttatgcgcaa tatcaatgtg atgcaggaat cctcagttgg acccgcattg agcatgaact
    23341 tcgacatgcg caaagacgtt cgcggcgtct ttatggcgaa aaaagaaaca ctgctgtctc
    23401 ttgcaaatgt tcatgcggtg aatgaaaaag ggcaaagctc cgtcgatatc gacagggtta
    23461 atcaccatat tgttaatgtg gaaaagatta actttagatt gccggaacgg agggagtaga
    23521 tttgcgacca ttcataaact tactggatat aatgttctaa aagattataa tcctcacact
    23581 aactgttaaa gctggggctg ggcagcattg actactcagg gaaggtactc atctttatct
    23641 tgtgtttctt acgtaacaac cagactaaag tgatttatga acgataacgt tttgctcata
    23701 ggagcttccg gattcgtagg aacccgacta cttgaaacgg caattgctga ctttaatatc
    23761 aagaacctgg acaaacagca gagccacttt tatcccaaaa tcacacagat tggcgatgtt
    23821 cgtgatcaac aggtactcga ccaggcgtta gctggttttg acactgttgt actactggca
    23881 gcggaacacc gcgatgacgt cagccctact tctctctatt atgatgtcaa cgttcagggt
    23941 acccgcaatg tgctggcggc catggaaaaa aatggcgtta aaaatatcat ctttaccagt
    24001 tccgttgctg tttatggttt gaacaaacac aaccctcacg aaaaccatcc acacgatcct
    24061 ttcaaccact acggcaaaag taagtggcag gcagaggaag tgctgcgtga atggtataac
    24121 aaagcaccaa cagaacgttc attaaccatc atccgtccta ccgttatttt cggtgaacgc
    24181 aatcgcggta acgtctataa cttgctgaaa cagatcgctg gcggcaagtt tatgatggtg
    24241 ggcgcaggga ctaactataa gtccatggct tatgttggaa acattgttga gtttatcaag
    24301 tacaaactga agaatgttgc cgcaggttat gaggtttata actacgttga taagccagac
    24361 ctgaacatga accagttggt tgctgaagtt gaacaaagcc tgaacaaaaa gatcccttct
    24421 atgcacttgc cttacccact aggaatgctg ggtggatatt gctttgatat cctgagcaaa
    24481 attacgggca aaaaatacgc tgtcagctca gtgcgcgtga aaaaattctg cgcaacaaca
    24541 cagtttgacg caacgaaagt gcattcttca ggttttgtgg caccgtatac gctgtcgcaa
    24601 ggtctggatc gaacactgca gtatgaattc gttcatgcca aaaaagacga cataacgttt
    24661 gtttctgagt aatgcagacg agtcagagaa aaaaggacca ggttggacta cgattattcg
    24721 gaaagaggta ctgattaaat tatcagtatg cggaattccg agtaatccga gtaacccaga
    24781 tgctgatggt gtgtagtgtt atcgcaaggt ttaatcaaag ttatgcggaa ctaagctttg
    24841 gtatatagcc ataccgtttg gctaacctat ggcaataatt tacgcactat cagccactta
    24901 gaggtaggaa aataatgacg aatttaaaag cagttatacc ggtagcgggt cttgggatgc
    24961 atatgttgcc tgccactaag gcgataccca aagagatgct accgatcgtc gacaagccaa
    25021 tgattcagta cattgttgac gagattgtgg ctgcagggat caaagaaatc ctcctggtaa
    25081 ctcatgcgtc caagaacgcg gtcgaaaacc acttcgacac ctcttatgaa ttagaatctc
    25141 tccttgaaca gcgcgtgaag cgtcaactgc tggcggaagt acaatctatc tgtccgccgg
    25201 gcgtgaccat tatgaacgtg cgtcagggtg aacctttagg tttgggccac tccattttgt
    25261 gtgcacgccc cgccattggc gacaacccat ttgtcgtggt gctgccggac gttgttatcg
    25321 atgatgccag cgccgacccg ctgcgctaca accttgctgc catgattgcg cgcttcaatg
    25381 aaacgggccg tagccaggtg ctggcaaaac gtatgccggg tgacctctct gaatactccg
    25441 tcattcagac caaagaacca ctggatcgtg aaggtaaagt cagtcgcatt gttgaattta
    25501 tcgaaaaacc ggatcagcca caaacgctgg actcagacat catggccgtt ggtcgctatg
    25561 tgctttctgc cgatatttgg ccggaacttg aacgcactca acctggtgca tgggggcgta
    25621 ttcagctgac tgatgccatt gctgaactgg cgaaaaaaca gtccgttgat gcaatgctga
    25681 tgactggtga cagctacgac tgcggtaaaa aaatgggcta tatgcaggcg tttgtgaagt
    25741 atggactacg caatctgaaa gaaggggtga agttccgtaa aggtattgag aaactgttaa
    25801 gcgaataatg aaaatctgac cggatgtaac ggttgataag aaaattataa cggcggtgaa
    25861 gattcctgtg gagagtaatt tgttgcgatt accactgtcg ttattcaact ataagcataa
    25921 aaaagttaaa tataatttag ggattttaag tatatggaaa cgcctttg
//
LOCUS       DAZKHK010000079        25680 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKHK010000079 DAZKHK010000000
VERSION     DAZKHK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.079"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(84..191)
                     /gene="yniD"
                     /locus_tag="ACHOS5_003155"
     CDS             complement(84..191)
                     /gene="yniD"
                     /locus_tag="ACHOS5_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP9777927.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(362..1390)
                     /locus_tag="ACHOS5_003156"
                     /pseudo
     CDS             complement(362..1390)
                     /locus_tag="ACHOS5_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            1913..3841
                     /gene="thrS"
                     /locus_tag="ACHOS5_003157"
     CDS             1913..3841
                     /gene="thrS"
                     /locus_tag="ACHOS5_003157"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9777928.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3845..4387
                     /gene="infC"
                     /locus_tag="ACHOS5_003158"
     CDS             3845..4387
                     /gene="infC"
                     /locus_tag="ACHOS5_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9777929.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4484..4681
                     /gene="rpmI"
                     /locus_tag="ACHOS5_003159"
     CDS             4484..4681
                     /gene="rpmI"
                     /locus_tag="ACHOS5_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9777930.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4734..5090
                     /gene="rplT"
                     /locus_tag="ACHOS5_003160"
     CDS             4734..5090
                     /gene="rplT"
                     /locus_tag="ACHOS5_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9777931.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5207..5331
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5213..5257
                     /gene="pheM"
                     /locus_tag="ACHOS5_003161"
     CDS             5213..5257
                     /gene="pheM"
                     /locus_tag="ACHOS5_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP9777932.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5541..6524
                     /gene="pheS"
                     /locus_tag="ACHOS5_003162"
     CDS             5541..6524
                     /gene="pheS"
                     /locus_tag="ACHOS5_003162"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9777933.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6539..8926
                     /gene="pheT"
                     /locus_tag="ACHOS5_003163"
     CDS             6539..8926
                     /gene="pheT"
                     /locus_tag="ACHOS5_003163"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9777934.1"
                     /translation="MKFSELWLREWVNPAIDSDGLASQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8931..9230
                     /gene="ihfA"
                     /locus_tag="ACHOS5_003164"
     CDS             8931..9230
                     /gene="ihfA"
                     /locus_tag="ACHOS5_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP9777935.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9331..10311
                     /gene="btuC"
                     /locus_tag="ACHOS5_003165"
     CDS             9331..10311
                     /gene="btuC"
                     /locus_tag="ACHOS5_003165"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP9777936.1"
                     /translation="MLTLARQQQRQNFRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10373..10924
                     /gene="btuE"
                     /locus_tag="ACHOS5_003166"
     CDS             10373..10924
                     /gene="btuE"
                     /locus_tag="ACHOS5_003166"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP9777937.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            10924..11673
                     /gene="btuD"
                     /locus_tag="ACHOS5_003167"
     CDS             10924..11673
                     /gene="btuD"
                     /locus_tag="ACHOS5_003167"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP9777938.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKK
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            11751..12215
                     /gene="nlpC"
                     /locus_tag="ACHOS5_003168"
     CDS             11751..12215
                     /gene="nlpC"
                     /locus_tag="ACHOS5_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9777939.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12462..13175
                     /gene="ydiV"
                     /locus_tag="ACHOS5_003169"
     CDS             12462..13175
                     /gene="ydiV"
                     /locus_tag="ACHOS5_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9777940.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRENFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13238..14674
                     /gene="selO"
                     /locus_tag="ACHOS5_003170"
     CDS             13238..14674
                     /gene="selO"
                     /locus_tag="ACHOS5_003170"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP9777941.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLDDEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRVRLQQDEVTDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(14678..14869)
                     /gene="hemP"
                     /locus_tag="ACHOS5_003171"
     CDS             complement(14678..14869)
                     /gene="hemP"
                     /locus_tag="ACHOS5_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP9777942.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15001..16047)
                     /gene="aroH"
                     /locus_tag="ACHOS5_003172"
     CDS             complement(15001..16047)
                     /gene="aroH"
                     /locus_tag="ACHOS5_003172"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP9777943.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQPRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIAGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16204..17037)
                     /gene="ppsR"
                     /locus_tag="ACHOS5_003173"
     CDS             complement(16204..17037)
                     /gene="ppsR"
                     /locus_tag="ACHOS5_003173"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP9777944.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17370..19748
                     /gene="ppsA"
                     /locus_tag="ACHOS5_003174"
     CDS             17370..19748
                     /gene="ppsA"
                     /locus_tag="ACHOS5_003174"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP9777945.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPLEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(19805..21451)
                     /gene="fadK"
                     /locus_tag="ACHOS5_003175"
     CDS             complement(19805..21451)
                     /gene="fadK"
                     /locus_tag="ACHOS5_003175"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP9777946.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITVHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21508..21801)
                     /gene="ydiT"
                     /locus_tag="ACHOS5_003176"
     CDS             complement(21508..21801)
                     /gene="ydiT"
                     /locus_tag="ACHOS5_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9777947.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(21798..23087)
                     /gene="ydiS"
                     /locus_tag="ACHOS5_003177"
     CDS             complement(21798..23087)
                     /gene="ydiS"
                     /locus_tag="ACHOS5_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9777948.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGSAASAPIERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLSDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23142..24080)
                     /gene="ydiR"
                     /locus_tag="ACHOS5_003178"
     CDS             complement(23142..24080)
                     /gene="ydiR"
                     /locus_tag="ACHOS5_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP9777949.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATTVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24100..24864)
                     /gene="ydiQ"
                     /locus_tag="ACHOS5_003179"
     CDS             complement(24100..24864)
                     /gene="ydiQ"
                     /locus_tag="ACHOS5_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP9777950.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEVA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGELLQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TDIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            25180..>25680
                     /locus_tag="ACHOS5_003180"
     CDS             25180..>25680
                     /locus_tag="ACHOS5_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9777951.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GVEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         6384 a         6137 c         6707 g         6452 t
ORIGIN      
        1 gacaacctta tcgttgggtt acagcatgta aaattatctg ccggaccgtg ttatgcggtc
       61 tggctgtttt cgtttgtcac tgctcagccc caaatcattg ctgcccaacg tagcaacaac
      121 atagcgccac agattgccag tagcaccacc accatcttcc agtgtttttt atcaaagcgt
      181 ttagtcggca taccagtgtc ctgcatatct cttgtgcgtg ataacggagt gtaacaaaag
      241 ggacaggcat caagggattt tttgttatta cagtcgtcgg gtggcgttgt acacctggtc
      301 actgtttctg cattttatga aaaaaattat gtatcgatat acaaatatca taaatagcgg
      361 gttataaata cgtctttagt tcatcagcac taaaactcag gctttggctg attacattta
      421 tagcagcata atatgtttct acagttttat gatgattatg atgaatggct atatgaacag
      481 ctgacatgtt gtcatgattt ttagcggcca acagtgtaaa taactgacgt tggctaaaag
      541 aatatttttt caaaagtacc caacgttgat atataagata acacgacatc tttacttact
      601 gacaaagtat gattgtctat atataaaatc gcggacatat gactgacatt gtttgccatc
      661 attcgacaga agaaaatatc taaagcaaag ccaaaatcct gactcgcgat aacgcgactc
      721 cttgcaagag gatggcgaag taatgctctg aattccgcgt cccggactaa gggaacatgc
      781 tgctgtatgg attcaatgga ggagtaatgt ttaatcaggt tgactttgcc ttttccattt
      841 ctcaatgaat caagggcgta ttttaatgac agatggcgac aaaaaatagt gtcagaagga
      901 gattgcaaaa aaatgcacta tcattgagat ttactgttgg ctgtatggtt ttttctctat
      961 gaattttttt ggacagtttc atgcaatggt tacgtttttc ggcagttaac tgttcctgtt
     1021 caatataatc cagtatgctt cgcaaatggt tagctttaat cggcgtggtt tccaggaata
     1081 aggaaatgat ttctttttta tagtgtaatg taatgagtgt attttctgca tggatgggtg
     1141 aagtggcaaa gttaatgata aaaagggcag tggaataaca attttgtgaa atttttcgac
     1201 agcttgcggt aaattcctga gtaacacagt atgacagcat cgaacttaaa taagaggtat
     1261 caagtagggt gatgagctgg cgcgcaaatt gattaatagg ctctgatgaa ggcgttagta
     1321 tgtcgctatc gatgtgcatt ctaagaggag actcataatg agttctgata atgatatcgt
     1381 ttttactcat aatctattcg ccttatatat gtctggagat tttgaggatg atgattgatt
     1441 attatcgcca gaacgaaatg cgcacgaaaa attgctataa gaattgtgtc acttttatca
     1501 ttgcgatatg actacttcgt cattaatctc atgatcgttt aaatactacc gccaaatcac
     1561 gcaacgaact ttgctggatt cagtttatgc tgctgtaaat ccgctcgtag taaacctttc
     1621 agacactctg tgatgttatc agtggttctt taagcgtttt gctggtgtac tcactacaaa
     1681 cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt tttagtgtta
     1741 acatcgttca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt acctgattgc
     1801 ggaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat aaacaaattt
     1861 ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata aaatgcctgt
     1921 tataactctt cctgatggca gccaacgcca ttacgaccac gctgtaagcc ccatggatgt
     1981 tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg ttaatggcga
     2041 actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca ttactgccaa
     2101 agacgaagaa ggtctggaga tcattcgtca ctcctgtgcg cacctgttag ggcacgcgat
     2161 taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca acggttttta
     2221 ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg
     2281 gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca gctggcatga
     2341 agcgcgtgaa actttcgcca accgtgggga gagctacaaa gtctccattc ttgacgaaaa
     2401 catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg atatgtgccg
     2461 cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga aaacggcagg
     2521 ggcttactgg cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg gtacggcgtg
     2581 ggcagacaaa aaagcactta acgcttacct gcagcgcctg gaagaagccg cgaaacgcga
     2641 ccaccgtaaa atcggtaaac aactcgacct gtaccatatg caggaagaag cgccgggtat
     2701 ggtattctgg cacaacgacg gctggacgat cttccgtgaa ctggaagtgt ttgttcgttc
     2761 taaactgaaa gagtaccagt atcaggaagt taaaggtccg ttcatgatgg accgtgtcct
     2821 gtgggaaaaa accggtcact gggacaacta caaagatgca atgttcacca cgtcttctga
     2881 gaaccgtgaa tactgcatta agccgatgaa ctgcccgggt cacgtacaaa ttttcaacca
     2941 ggggctgaag tcgtatcgcg atttgccgct gcgtatggcc gagtttggca gctgccaccg
     3001 taacgagcca tcaggttcgc tgcatggcct gatgcgtgta cgtggtttta cccaggatga
     3061 cgcgcatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat gtatccgttt
     3121 agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac tctccactcg
     3181 tcctgaaaaa cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt
     3241 tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg ctttctacgg
     3301 tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt gcggtacagt
     3361 acagctggac ttctccttgc cgtctcgtct gagcgcctcc tatgtgggcg aagacaacga
     3421 gcgtaaggta ccggtaatga ttcaccgcgc aattctgggg tcgatggaac gtttcatcgg
     3481 tatcctgacc gaagaattcg ctggtttctt cccgacctgg cttgcgccgg ttcaggttgt
     3541 tatcatgaat attaccgatt cacagtctga atacgttaac gaattgacgc aaaaactatc
     3601 aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct ttaaaatccg
     3661 cgagcacact ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag aggtggaatc
     3721 aggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg acgtaaatga
     3781 agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat tggaggaata
     3841 aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca atggcgaaat
     3901 tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta ttgtgagtct
     3961 gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga tcagccctaa
     4021 cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg aaaagagcaa
     4081 gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa ttaaattccg
     4141 tcctggtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc gctttctcga
     4201 agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca
     4261 aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg cagtggtcga
     4321 atccttccca acgaagatcg aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa
     4381 acagtaaggc cttcaagtag cactgtctgt ggagcctttg ggcttcacag gcattgttcg
     4441 cctttgtttc gtttattaac aatgcgaagt ggaagttatt aaaatgccaa aaattaagac
     4501 cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca
     4561 cgctaacctg cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc
     4621 gaaagccatg gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata
     4681 agccgttaac gtttttaact ttttaattag aatatagata caggagagca catatggctc
     4741 gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa caagctaaag
     4801 gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg
     4861 gtcagtatgc ttaccgcgac cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg
     4921 cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta cagcaaattc atcaatggcc
     4981 tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc tgatatcgca gtattcgaca
     5041 aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc tctggcataa gccagttgaa
     5101 agagggagct agtctccctc ttttcgtttc aacgccatca aaacattgac ttttatcgcc
     5161 gtagcctttt caataaaggt cttttgaaga gtaccaaaag gtaacgcaag caatgaatgc
     5221 tgctattttc cgcttctttt tttactttag cacctgactc caggaggcta gcgcgtgaga
     5281 agagaaacgg aaaacagcgc ctgaaagcct cccagtggag gctttttttg tatgcgcgtt
     5341 ttgaaaaata aaaagcctgt ctcatcaggc gtaattgtcg cagccagtct ggacacggcc
     5401 agcgcggaga aaccggagcg tacttaagta cgtgaggatt tcgagcactg cccgggacca
     5461 aaatggcaag taaaatagcc tgatgggatc ggctctaagt ccaacgaacc agtgtcacca
     5521 ctgacacaat gaggaaaaac atgtcacatc tcgcagaact ggttgccagt gcgaaggcgg
     5581 ccattagcca ggcgtcagat gttgccgcgt tagataatgt gcgcgtcgaa tatttgggta
     5641 aaaaagggca cttaaccctt cagatgacga ccctgcgtga gctgccgcca gaagagcgtc
     5701 cggcagctgg tgcggttatc aacgaagcga aagagcaggt tcagcaggcg ctgaatgcgc
     5761 gtaaagcgga actggaaagc gctgcactga atgcgcgtct ggcggcggaa acgattgatg
     5821 tctccctgcc gggtcgtcgc attgaaaacg gcgggctgca tccggtgacc cgtaccatcg
     5881 accgtatcga aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg ccggaaatcg
     5941 aagacgatta tcataacttc gatgctctga acattcctgg tcaccacccg gcgcgcgctg
     6001 accacgacac tttttggttt gacgctaccc gcctgctgcg tacccagacc tctggcgtac
     6061 agatccgcac catgaaagcc cagcagccac cgattcgtat catcgcgcct ggccgcgttt
     6121 atcgtaacga ctacgaccag actcacacgc cgatgttcca tcagatggaa ggtctgattg
     6181 ttgataccaa catcagcttt accaacctga aaggcacgct gcacgacttc ctgcgtaact
     6241 tctttgagga agatttgcag atccgcttcc gtccttccta cttcccgttt accgaacctt
     6301 ctgcagaagt ggacgtcatg gggaaaaacg gtaaatggct ggaagtactg ggctgcggga
     6361 tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc ggaagtttac tctggtttcg
     6421 ccttcgggat ggggatggag cgtctgacca tgttgcgtta cggcgtcacc gacctgcgtt
     6481 cattcttcga aaacgatctg cgtttcctca aacagtttaa ataaggcagg aatagattat
     6541 gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg gcgattgata gcgacgggct
     6601 ggcaagtcaa atcactatgg cgggcctgga agttgatggt gtagaaccgg ttgccggtag
     6661 cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg cagcatccga acgctgacaa
     6721 actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg ctggacatcg tctgcggtgc
     6781 gccaaactgc cgtcagggcc tgcgtgtggc ggtagcgacc attggtgctg ttctgccggg
     6841 tgatttcaaa attaaagccg cgaaactgcg tggcgaaccg tctgaaggga tgctgtgctc
     6901 cttctctgag ctgggaattt ctgacgatca taacggcatt attgagctgc ctgcggatgc
     6961 gccaattggc accgacattc gtgaatacct gaaactcgat gacaacacca tcgaaatcag
     7021 cgtgacacca aaccgtgccg actgcttagg tatcattggt gttgcgcgcg acgttgccgt
     7081 gttgaaccag ctgccgctgg ttgaaccgga aatcgttccg gttggtgcga ccatcgacga
     7141 cacgctgccg attgcagtag aagcgccgga tgcctgcccg cgctatcttg gccgtgtggt
     7201 aaaaggcatt aacgttaaag cgccaactcc actgtggatg aaagaaaaac tgcgtcgttg
     7261 cgggatccgt tctatcgatg cagttgttga cgtcaccaac tatgtgctgc tcgaactggg
     7321 ccagccgatg cacgctttcg ataaagatcg cattgaaggc ggcattgtgg tgcggatggc
     7381 aaaagagggt gaaacgctgg tattgctcga tggtactgaa gcgaagctga atgctgacac
     7441 cctggtcatc gccgaccaca acaaggcgct ggcgatgggc ggcatctttg gtggcgaaca
     7501 ctctggcgtg aatgacgaaa cgcaaaacgt cctgctggaa tgcgctttct tcagcccgct
     7561 gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact gatgcgtctc accgttatga
     7621 gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa cgtgcgaccc gtctgctgat
     7681 cgacatctgc ggtggtgaag cgggtccggt aattgatatc actaacgaag caacgctgcc
     7741 gaagcgtgca accatcactt tacgtcgtag caaactggat cgcctgatcg gccatcatat
     7801 tgctgatgag caggtaacag acattctgcg tcgtctcggc tgcgaagtga ccgaaggcaa
     7861 agacgagtgg caggcagttg cgccgagctg gcgtttcgac atggagattg aagaagatct
     7921 ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc ccggatgagc cggtacaggc
     7981 aagcctgatt atggggactc accgtgaagc tgacctgtcg ctcaagcgcg taaaaacgct
     8041 gctcaacgac aaaggctatc aggaagtgat cacctacagc ttcgttgatc cgaaagtgca
     8101 gcagatgatc catccaggcg ttgaagcctt actgctgcca agcccgatct ctgttgaaat
     8161 gtcagcaatg cgtctttctc tgtggaccgg cctgctggca accgtggtgt acaaccagaa
     8221 ccgtcagcag aatcgtgtac gcattttcga aagcggtctg cgtttcgtgc cagatactca
     8281 ggcaccgttg ggtattcgtc aggatctgat gttagccggt gtgatttgcg gtaaccgtta
     8341 cgaagagcac tggaacctgg caaaagagac cgttgatttc tatgatttga aaggcgatct
     8401 tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt gagttccgtg cagaagcgaa
     8461 tccggcactg catccggggc aatccgcagc gatttatctg aaaggtgaac gtattggttt
     8521 tgttggggtt gttcatcctg aactggaacg taaactggat cttaacggtc gcactctggt
     8581 gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg cctcaggcgc gcgagatttc
     8641 tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc gcagaaaacg ttcccgcagc
     8701 ggatatttta tccgaatgta agaaagttgg cgtaaatcag gtagttggcg taaacttatt
     8761 tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag agcctcgcca taagcctgat
     8821 cctgcaagat accagccgta cactcgaaga agaggagatt gccgctaccg tcgccaaatg
     8881 tgtagaggca ttaaaagagc gattccaggc atcattgagg gattgaacct atggcgctta
     8941 caaaagctga aatgtcagaa tatctgtttg ataagcttgg gcttagcaag cgggatgcca
     9001 aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc tctggaaaac ggcgaacagg
     9061 tgaaactctc tggttttggt aacttcgatc tgcgtgataa gaatcaacgc ccgggacgta
     9121 acccgaaaac gggcgaggat attcccatta cagcacggcg cgtggtgacc ttcagacccg
     9181 ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa agacgagtaa tctgatctaa
     9241 ctaaaaaggc cgctctgcgg ccttttttct tttcactgtc gaagagtcac cgtaaaatca
     9301 acgccatgac acttcagcag aacggatacc atgctgacac ttgcccgcca acaacagcga
     9361 caaaattttc gctggttatt atgcctgtca gttttgatgc tgctggcgct tctcttaagc
     9421 ctttgcgccg gtgaactatg gattttgcca ggtgactggt tttctcctcg tggcgaactg
     9481 ttcgtctggc aaattcgcct gccacgtacg ctggctgtat tgctggttgg tgcggcgctg
     9541 gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc ctctggcaga acctggacta
     9601 cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg cggtattgct tgggcaaggg
     9661 caactcccca actgggcgct agggctgtgt gcgattgctg gcgcgcttat catcacttta
     9721 atactcttac gtttcgctcg tcgtcatctt tcgaccagtc ggttattgct ggctggcgtt
     9781 gcattaggga ttatctgtag cgcactaatg acgtgggcta tctacttttc cacctctgtt
     9841 gatttacgtc agctgatgta ctggatgatg ggcggttttg gcggcgtaga ctggcggcaa
     9901 agctggctga tgctggcatt gatccccgtg ttgttgtgga tctgttgtca gtccaggccg
     9961 atgaatatgt tagcacttgg cgagatctcg gcgcggcaac tgggtttacc cctgtggttc
    10021 tggcgcaatg tgctggtggc agcgaccggc tggatggttg gcgtcagtgt ggcgctggcg
    10081 ggtgctatcg gctttattgg tctggtgatc ccacatattc tccggctgtg tggtttaacc
    10141 gatcatcgcg tattacttcc cggctgcgcg ctggcagggg cgagcgcatt gctgctggcc
    10201 gatattgtag cgcgcctggc attagctgcc gcagagctgc caattggcgt ggtcaccgca
    10261 acgttgggtg cgccggtgtt tatctggtta ttgttaaaag caggacgtta gccgcaaaag
    10321 acggtctatg attaaaagct aagattttta ctgaccacac ccaggagaaa cgatgcaaga
    10381 ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc tggagaagta
    10441 cgccggtaat gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata
    10501 tgagcagttg gagaatattc agaaagcctg ggctgatcgt ggttttgtgg tgctgggatt
    10561 cccgtgcaac cagtttctgg agcaagaacc gggtagcgat gaagagatta aaacttactg
    10621 caccaccaca tggggggtga cgttcccgat gttcagtaag attgaagtta atggcgaagg
    10681 acgccatccg ctgtatcaaa aattgattgc cgcagcgccg actgcagtcg cgccggaaga
    10741 gagcggattc tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc ctgatgatat
    10801 tttatggaat tttgagaaat ttttagttgg cagggacgga aaggtcatcc agcgtttttc
    10861 cccggatatg acgccggaag atcccattgt gatggaaagc attaaactgg cgttggcaaa
    10921 ataatgtcta ttgtgatgca gttacaggat gttgcggaat ccacccgcct ggggccgctt
    10981 tctggcgagg ttcgggctgg ggagatcctg cacctggtgg ggccgaatgg cgcgggtaag
    11041 agtaccttac tggcgcgaat ggccggaatg accagcggta agggaagcat tcagttcgcg
    11101 gggcaaccac tggaagcatg gtctgcaaca aagctcgcgc tgcatcgcgc ctatctttca
    11161 caacagcaga cgccgccgtt tgcaatgccg gtctggcact acctgacact gcatcagcac
    11221 gataaaaagc gtaccgaact actgaatgat gtcgcagggg cgctggctct tgatgacaaa
    11281 ctcggacgta gcaccaatca actttccggc ggtgaatggc aacgcgtacg tcttgctgcg
    11341 gtggtgctgc aaatcacacc acaagccaat cccgcaggcc aattgctgct tcttgatgag
    11401 ccgatgaaca gtcttgatgt tgcgcaacaa agtgccttag acaaaattct gagcgcgctg
    11461 tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccacac attgcgtcat
    11521 gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg cagagaagag
    11581 gtgctcacgc cggcaaatct ggcgcaggcc tatgggatga attttcgccg tctggatatc
    11641 gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcttga aaatcgggta
    11701 attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga atgcgtttct
    11761 gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc
    11821 caaatgccag actttctgat tcgattaccg ttattgccgg tttgaacgac cagctacaaa
    11881 gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg
    11941 gatttgtggt tgtgacgatg cgcgatcgtt tcgatttgca gctgccccgt gaaaccaaac
    12001 aacaagcctc tattggcacg caaattgata aagacgagtt gctgccaggt gacttggtct
    12061 ttttcaaaac gggttccgga caaaatggtt tgcatgtagg tatttacgat accaacaacc
    12121 aatttatcca cgcctctacc agcaagggag tgatgcgttc ctcacttgat aatgtctatt
    12181 ggcagaaaaa tttctggcag gcgagacgaa tctagtatgt cgaaaaatgg acggcgatga
    12241 tacatcgccg tccatttact cctgaataca gtagtatttt gcagacaaaa attccgtaaa
    12301 tggattcttc gtttataatc aggataatct aaaaaaagga cccctgaaaa agtggttaac
    12361 atggaacaaa tgaaagttac tccttctgta attgttatgt cttatggcct catccctttt
    12421 agccggatac tgaaaaacat ccttcgagag ggacggttac catgaagatt tttttggaga
    12481 atctttatca ttctgattgc tatttcctgc cgataagaga taaccagcaa gaccttgtcg
    12541 gcgttgagtt aattacccat ttctccagtg aagatggtac ggttcgtatc ccgacgagtc
    12601 gggttatagc gcaacttacc gaggagcaac attggcaatt attttctgaa cagttggaat
    12661 tactaaaatc atgccaacac ttttttattc aacataaact ctttgcctgg cttaatttaa
    12721 cgccacaagt ggctacatta ttattagaca gggaaaattt cgcaggcgaa ctattaaaat
    12781 atccgtttat tgaactgttg attaatgaga attacccgca tttcaatgaa ggaaaagata
    12841 atcgcgatct tttatcctta tcgcaaatgt atccccttgt gttaggtaat ttgggtgcag
    12901 gtaatagcac aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg ctggataaaa
    12961 gttttattca gcaacaaatc acgcatcgtt ccttcgaacc gtttattcgt gcgatccagg
    13021 cgcaaatttc cccttgttgc aattgcatca tcgcgggggg cattgatacg ccagaaatat
    13081 tggcacagat tatccccttt gatttccatg ctctccaggg atgcctgtgg cctgcggttc
    13141 ctataaatca gataacgacg ttggttcagc gataaccctc ctgtttgctg gtgtttagga
    13201 cgagagtaac cgtctacact atcaaacagg aggatctatg accctgtctt ttattacccg
    13261 ctggcgcgat gaattgccag aaacctatac agcactttcc cctacgcctt taaataatgc
    13321 ccggctgatt tggcataata ccgaactggc taacacgctg agtattccat cgtcgctgtt
    13381 taaaaatggc gcaggtgtct ggggcggcga aaacctactg cctggtatgt caccacttgc
    13441 gcaggtttac agtggtcatc agtttggcgt ctgggcgggc caactgggtg atgggcgcgg
    13501 cattttactc ggcgaacaac tgcttgctga tggcactaca atggactggc atctgaaagg
    13561 tgctggcctg acgccttatt cgcgaatggg ggatggacgg gcggttttac gttcgacgat
    13621 acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga cccgcgcttt
    13681 aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtggagtcag gcgcgatgct
    13741 gatgcgtgtg gcaccaagtc atctgcgctt tggtcatttc gaacattttt actatcgccg
    13801 cgagccggaa aaggttcgtc agttagctga ctttgccatt cgtcattact ggtcacatct
    13861 tgatgatgag gaggacaaat accgtctctg gtttaccgat gttgtcgcac gtaccgcctc
    13921 gttaattgcc caatggcaga cggtcggctt tgctcatgga gtgatgaata ccgacaacat
    13981 gtcgctgctg gggctgacgc ttgattacgg gccgtttggt tttcttgatg attacgaacc
    14041 cggttttatt tgtaatcact cggatcatca agggcgttac agctttgata atcaacctgc
    14101 cgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg ttgccgttga
    14161 tgccctgaat gaggccctgg acagctatca gcaggttttg ttgacgcatt atggacaacg
    14221 gatgcggcag aaactgggct tcatgacgga gcaaaaagag gataacgcgc tactgaatga
    14281 attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacattcc gcatgctgag
    14341 tctgaccgag cagcacagcg cggcgtcacc gctacgtgat gagtttattg atcgtgcggc
    14401 atttgatgac tggtttgccc gttatcgggt acgtttgcaa caagacgagg ttaccgatag
    14461 tgagcgtcag cagctgatgc aaagcgttaa cccggctctg gtgttgcgca actggttggc
    14521 gcaacgggcg attgaagcgg cagaaaaggg tgatatgacg gaattgcacc gcctgcatga
    14581 ggcgttgcga aatcctttca gcgacagaga tgatgactat gtcagtcgtc cacccgactg
    14641 gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttggtcaac agcagcttgc
    14701 cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc agtttgccat
    14761 ccggacctaa cagtgtctgg ctggtaattc gccggatggg ctgcggggaa gcggtcttgt
    14821 gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata aaaattaata
    14881 ataatttata taacaatgat tcttattatc aatattatgg cggaccagag caagcagtaa
    14941 tttgttgaga agacaacaag ttgcgcagaa atgcccatcc cgtcaggaat gggcacgcat
    15001 tcagaagcga gtatctaccg cagaggcgag tttttcgacc aggcgttcgg tatcttccca
    15061 gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc cagcgatttt
    15121 ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatcgccg tagagccatt
    15181 gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct gcttctggca
    15241 gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc
    15301 gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccaccac gcataataat
    15361 gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt tgtctggcga
    15421 gaggaacata tggctggcgc gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc
    15481 atcggtacca tttttaaaac ccaccggaca ggagagtgcc gaagccattt cgcggtggat
    15541 ctgactttcg gtagtacgtg cgccaatcgc accccaactg attaaatcag caataaactg
    15601 accggtcacc atatcgagga actcagtcgc ggtagggacg cccagctcat ttacctgtaa
    15661 aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctaccgt ttaaatctgg
    15721 atcggagatg agtcctttcc agccgacaac cgttcgtggt ttttcaaaat aggtgcgcat
    15781 tacgatttcc agccgtggct ggtactggtt gcgtagcgac tgcagacggg tggcgtactc
    15841 catcgcagcg gtgagatcgt ggatcgagca ggggccaata atgaccaaca gtcgcttatc
    15901 ttcaccattc agtatttttt caattctacg gcgggagtcg gtgacatggg tggcgacgcc
    15961 aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct caatacgcgc
    16021 agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc ttctacggca
    16081 ggactaccgg gaagtgatgt gcatcacgat aaaccaatcg cagctgattt caagtgcgag
    16141 gtgtgtcaca cctcgccggg tggttagcat gataacaaaa aagtaagcta atgcactagt
    16201 tctctagtac attcggcggc taaggcccat gatatcgagg atcttggtgg caatctcttc
    16261 taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca acgcttccac
    16321 ttccgcgact tccatcctgc actgacgaag cgaggcatag cgactgttct cccgacgttc
    16381 ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt tgtgctgaag
    16441 tggtttgagc gacgcgggta gcaccagatt atccatatcg tcggcaataa aggggtaatt
    16501 tgccgcgcgg ataccaaatt gcattgccag atacagactg gtgggggttt taccacagcg
    16561 agaaacaccg agcaggatca cctgagcctg gtccagattg cgcaacgaaa taccgtcatc
    16621 gtgggcaagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga gattattagg
    16681 gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct cttgttgtag
    16741 cggggcaacc agcgcctgaa cgatatcctg gcaaaagcct tcactttgca agatgatagc
    16801 gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacacctg tctggtgata
    16861 aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga caaacggcag
    16921 cgtaatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta atacctccgc
    16981 agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat tatccatttt
    17041 gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg cacagaagcg
    17101 tagaacgtta tgtctggttt ataaaatgaa acttaaattt tattttttat gaaaacagca
    17161 tttcattttt atggtttcgt ttataccgat ggtttatgtg gaaattgtcg aagagagcag
    17221 atttgcgcaa cgctgggatc agtcttaaaa agtaaaaaaa tatatttgct tgaacgattc
    17281 accgtttttt tcatccggtt aaatatgcaa agataaatgc gcagaaatgt gtttctcaaa
    17341 ccgttcattt atcacaaaag gattgttcga tgtccaacaa tggctcgtca ccgctggtgc
    17401 tttggtataa ccaactcggc atgaatgatg tagacagggt tgggggcaaa aatgcctccc
    17461 tgggtgaaat gattactaac ctttccggaa tgggtgtttc cgttccgaat ggtttcgcca
    17521 caaccgccga cgcgtttaac cagtttctgg accaaagcgg cgtaaaccag cgcatttatg
    17581 aactgctgga taaaacggat attgacgatg ttacccagct tgcgaaagcg ggcgcgcaaa
    17641 tccgccagtg gattatcgac actcccttcc agcctgagct ggaaaacgcc atccgcgaag
    17701 cctatgcaca gctttccgcc gatgacgaaa acgcttcgtt tgcggtgcgt tcctccgcta
    17761 ctgcagaaga tatgccggac gcttcttttg ccggtcagca ggaaactttc ctcaacgttc
    17821 agggttttga cgccgttctc gtggcagtga aacatgtatt tgcttctctg tttaacgatc
    17881 gcgccatctc ttatcgtgtg caccagggtt acgatcaccg tggcgtggcg ctctccgccg
    17941 gtgttcagcg gatggtgcgc tccgacctcg catcttctgg cgtgatgttc tccattgata
    18001 ccgaatccgg ctttgaccag gtggtgttta tcacttccgc atggggcctt ggtgaaatgg
    18061 tcgtgcaggg tgcggttaac ccggatgagt tttatgtgca taaaccgaca cttgcggcga
    18121 atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa aatccgcatg gtttacgcgc
    18181 cgacccagga gcacggcaag caggttaaaa tcgaagacgt accgctggaa cagcgtgaca
    18241 tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa acaggccgta caaattgaga
    18301 aacactacgg tcgcccgatg gatattgagt gggcgaaaga tggccacacc ggcaaactgt
    18361 tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg tcaggtcatg gagcgttata
    18421 cgctgcattc acagggtaag attatcgccg aaggccgtgc tatcggtcat cgcatcggcg
    18481 cgggtccggt gaaagtcatc catgacatca gcgaaatgaa ccgcatcgaa cctggcgacg
    18541 tgctggttac tgacatgacc gacccggact gggaaccgat catgaagaaa gcatctgcca
    18601 tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat catcgctcgt gaactcggca
    18661 ttccggcggt agtgggctgt ggtgatgcaa cagaacggat gaaagacggc gagaacgtca
    18721 ctgtttcttg tgccgaaggt gataccggtt acgtctatgc ggagttgctg gaatttagcg
    18781 tgaaaagctc cagcgtagaa acgatgccgg acctgccgtt gaaggtgatg atgaacgtcg
    18841 gtaacccgga ccgtgctttc gacttcgcct gcctgccgaa cgaaggcgtg ggccttgcgc
    18901 gtctggaatt tatcatcaac cgtatgattg gcgtccaccc acgcgcactg cttgagtttg
    18961 acgatcagga accgcagttg caaaacgaaa tccgcgagat gatgaaaggt tttgattctc
    19021 cgcgtgaatt ttacgttggt cgtctgactg aagggatcgc gacgctgggt gccgcgtttt
    19081 atccgaagcg cgtcattgtc cgtctttctg attttaaatc gaacgaatat gccaatctgg
    19141 tcggtggtga gcgttacgag ccagatgaag agaacccgat gctcggcttc cgtggcgcgg
    19201 gccgctatgt ttccgacagc ttccgcgact gcttcgcgct ggagtgtgaa gcagtgaaac
    19261 gtgtgcgcaa cgacatgggg ctgactaacg ttgagatcat gatcccgttc gtgcgtaccg
    19321 ttgatcaggc gaaagcggtg gttgaggaac tggcgcgtca ggggctgaaa cgtggcgaga
    19381 acgggctgaa aatcatcatg atgtgtgaaa ttccgtccaa cgccttgctg gcagagcagt
    19441 tcctggaata tttcgacggc ttctcaattg gctcaaacga catgacacag ctggcgctcg
    19501 gtctggaccg tgactccggc gtggtgtctg aactgttcga tgagcgcaac gatgcggtga
    19561 aagcactgct gtcgatggcg attcgtgccg cgaagaaaca gggcaaatat gtcgggattt
    19621 gcggtcaggg tccatccgac cacgaagact ttgccgcatg gttgatggaa gaggggatcg
    19681 atagcctgtc tctgaacccg gacaccgtgg tgcaaacctg gttaagcctg gctgaactga
    19741 agaaataaaa taaatacccg gcggcgtttt gtcgccgggt ttatgtgatc cccgaagatg
    19801 aaacttattc aatctcttca cagacatcct gagttaatcg ccgcataata tctttcctta
    19861 acaaaaattt ttgtatttta cctgagacag tgcgcggtag tttttcgatt accacgatat
    19921 gttcaggata tttatatttc gcgacccgtt tacggctaaa aaaagccacc acctcttcca
    19981 gcgataatga atgatgcggt gctttcagca cgacataagc gcatgaacgt tcacctaagc
    20041 gttcatcggg cattgcaacc acacaagcat cgtgaatttt aggatgctgc aataaaatat
    20101 cttccacttc acggctgcta atattttcgc cgccgcggac aataatatct ttcttgcgcc
    20161 cggttatttt tatatagcca gcctcatcca tgcggcagag atcgccgctg taataccagc
    20221 cttcttcatc cagcgcacgg gcggttaatt caggttcatc aaaatacccc ataaacacat
    20281 tggggccacg cgaggcttct tcaccttcgc aacctggcgg taaagtcttg cgtgcgtcat
    20341 cgaccacttt aatctctaca cctgcggcag cgtaaccgtc ggtgtgcata aagcgcggca
    20401 aaggatcgtc gagattcacc accgcatgcg gcgaactttc tgtggaacca taaacactta
    20461 ataatttaat gccgcgctgc tggcattcac gcgccacttt tttggggatt gtggtaccgc
    20521 cgcaaagaaa gaaacgcagc gctgaaaggt ctgcgggctg tttctctagt aaattcaaaa
    20581 gatcatagac aaacggcgtt gcgccgagca tacaggtgca acgctgctgc tcaagcagcg
    20641 cgagacacgc agcaggagtg aaaatatcta acaacacgct gcgagcacca atcaaaaaag
    20701 gtgctgttac gccatgcaga aagcccgttg cgtgaccaag tggcgcaggc atcataaaga
    20761 catcctgcca ggtcagattt agccgcgcgc aataagcccg ctcactggcg agaatattgt
    20821 tatgcgttag catcacaccc tttggcagac cctcggttcc ggaggtaaat agcacagcgg
    20881 ctaattcatc gccgtggacc gttatcgccg tggtcagagg agtattgtcg gcgagaatct
    20941 gacttaatga gagggaagag gtggcgggag ccagtttgtc cacgccgaca atttgttgta
    21001 gttgtggaag ctgattttgc agcggcagga ttaaatctac cggacgcgtt tgtttaaaca
    21061 acgtcggtgc aaagaacatt tttgcctgac acttattgag cacccacacc agttctgctt
    21121 cccgccagga aggtaacagc ggcacggaaa ccgcaccggt tttcaggcag gcaagataga
    21181 taacggtaaa ttcacaccag cctggcagtt gaaatgcgat gcgatcgcct gattcaatac
    21241 ccttcgctaa catccagttt gccagacagc tcgcggcgtg atcgagcgcg ctataggtgt
    21301 acgttgcacc atgattgtcg accacggcaa ttttgtctgg catcgcacga gcggtctgct
    21361 gccagtaatc ggccagcgaa gcatcgcccc ataacccttg ctgacgatac gccgcacgac
    21421 gttgttcgtt gaacgttaat gtcactttca ttttggactc tcgaaacaga acatcaggcc
    21481 cgagatgcgg gcctgtggga tgcatgatta gccgtagcga aattcaatac cgaaggtgcc
    21541 tgcgggatat tgccactgtt cgagaatagt gttgccgcac agcacccgac aggtgccgca
    21601 ctccagacaa ccggcggaat cgaaatgaat gtttcctgcg tcatcctgct tataaagtcc
    21661 ggcagggcaa gctttcatta atttacggaa ttcattgata tcgggatttg ccgccaaaat
    21721 gatatgcggg tggccctcat caacatggaa tttattgacg cctaatttga tgtcgacgtt
    21781 aaccgtagcg ttctggctca tagcgcggtt gctcccttaa tgccatcttt cagcaagttg
    21841 atcagcccaa ttttcttcgc gtgtcccatg atcattttgc gtaccggctg gtttggtttg
    21901 ccgtcaatgg tgaacatatc gttcatgatg tcggcgacca ttcgtgggta ttggctaaac
    21961 aggcgcgggt tttccatcag cgccgggatc ttgcggaaat gctgcatatc gcgcataaca
    22021 caactttgtt ccagctcacg tttgtattgc gccagactgc tggcggagaa atccgcgcgt
    22081 tctttggcgg cgatcaccgt tgtggcggca gcctgagctg atgcaatggc taaatccatg
    22141 ccgcgaactg taaaacccaa attcaggcag aagcctgcgg cgtcaccaac gatcatcacg
    22201 ccatcgttaa ccagctgcgg caccattgcc agaccgccct ccggcaccat atgcgcggaa
    22261 tattcaagca gtttgccgcc gctaatcagc gggcgaatgg cggggtgttg tttaaaatct
    22321 tccagcattt gcggcacgct tttttgcgca tgggcgatat cactcaatcc acaaaccagc
    22381 cccaacgata cggaatcttt gttggtatag aggaatccgc cgcccatcag gccgtcagaa
    22441 ggggaaccgg caaacagcca ggcggcacct tcctcgcccg taacattaaa gcgatcgttg
    22501 atctgttctg gtgtcaggcc tataacctct ttaacaccaa cagcgtaatg atgcggattg
    22561 gaagcggaaa ccattcccag cgagcggcca agcatcgagt taacgccatc agccagaatc
    22621 accacattcg cttcgagaat atcatccccg gcctggacgc cagtgacctt gtttccttca
    22681 cgaaccagcg catcgacgcg tacgcccggg ataaactgcg cgcccgcctg ctcggcttgt
    22741 tccatcaacc acgggtccag acgattacgc aatacggtat aagacgcgtg ttgcggaaca
    22801 tctggttgct cgcggtgaaa atcgagggta acggcgcttt cttcggttaa gaaggagatt
    22861 ttctcgcgtg tgaccttgcg ttctatcggc gctgatgctg cagagcctgg aataattgct
    22921 tcgagtgtgt gggcataaag acgcccgccg gtcatgtttt tacatccggc actgtcgccg
    22981 cgttctatca ccaggacatc cagcccggct cgcgccatga ccagtgcggc aacgctaccg
    23041 gcaacgcccg caccgaccac aatggcatca aatttgtcat ccgacatgcg ttatgctccc
    23101 ttcccgctgc gtttacagcg ggagatgtgg ttgaaaggga attagcgact caactggcta
    23161 atcagggcag ggacgacttt gtagatatcg cccaccagac cgtagtcggc atagttgaag
    23221 attggcgcat ttttatcttt attaatggcg acaatcactt ttgcgccgtt gccaccaacc
    23281 atatgctgga tctgcccgga gatcccaagc gtcaggtaga gatcggattt cagcaacacg
    23341 ccggagacac cgatataacg ttcacgctcc atccagttct cgccttcggc aattggacgt
    23401 gaacagccga cttcagcatt cagcaccgcc gccagttcgt ggaccatttt caggtcatcc
    23461 tgcgccgcca gaccacgacc gacgccaacc acacgttttg ctttgctcag gtccacgctg
    23521 cttgccgctt tcgcacggcg ttcgcgacag agaatttcat gacgcggggc aacataaggt
    23581 accgtttctg tcgggcactg atgagagata tcactggtgc acggctcctg aacaccgggt
    23641 gcaagggtaa tgatcgccag cgggctgttg atcttttcct gagcgaacgc taacccgcca
    23701 tacatccggt gttcggcgca aatgtgacca tcgacaatat ccaccgtcgt ggcatcgttc
    23761 accagcgccg cattcagttg cacacttaac cgtgcggcca gagctttacc acgtttcgtc
    23821 gcggccaaca gcaacatagc ggggtgttta tctttcagca gggcagcaat gctttcggcg
    23881 taattttcag tacgttgcag cgcgtcgttt tgctcaagaa cataaatgca ttttggccca
    23941 taaggcataa ctgcctgcgc ctgctcggta ttttgtacaa tggcatacac ctgttggccc
    24001 cattgctgag cgccgccaaa cagttcagca taacgttcag gattatcgct aaagacccag
    24061 acgctgttta attgactcat cgtttcttct ccgtaggctt cagttcaggg cttttttcag
    24121 atgttccgcc agctcggcaa tggcctccgg cgaatcgtta tcgagaatga tgtgcttacg
    24181 ttctgtttgc ggcggtacgc gaatgtcggt cagttcagca agtggtgcgc tctggctcca
    24241 gccaatatca cttgcctgcc actgatttac cggtttttta cccgcgccga ggatggcttt
    24301 catcgaagga atacgtggta cgttaatatc ggaggtgacg cagagcacgg ctggaacaga
    24361 gagttcaata acttcaacat catcttcaag cgtgcgttca atcaccagtg tattgccctg
    24421 acgctgaatg gcactcactg cattaatcac cggaagttgc agaagttctc cgaccagcaa
    24481 gccaacctgc tgggcataaa ggtcgccgga accttcacca aagatcagta aatcgaagcc
    24541 gatcttttca actgctgtcg ccagcgcctt tgcggtatcg agaggcagtg catgctcaag
    24601 ttgtgcatcc tgcaccatat acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac
    24661 tttcgagttc tgcaacaaag agccgccgac ggtcagcgcg gctatctcat cgtcatctgt
    24721 tgcaagctgg cttgcaactt caatggcatt gagatcgaac tggctgattt tggcgtcggc
    24781 attgtcgaaa ttcagggtgt attctggagt gacaacaatg tcctgttctt caggcaccag
    24841 cttaaagcag gttattattt tcatgatttc tcctgtgagt cgcatacgga accatctccg
    24901 atggaaggta aaaatggcct gaaaatagtg tgcgatagcg gtgcggggtt ttcgacggag
    24961 caagctgttt tccttgccaa caatcttgca aaaaaaatgc gatcgctaaa acaaaagtgc
    25021 aatttgcggc aatatagtta ttccatgacc gtgcaatctt gcattcagtg taaataatct
    25081 tgtttagctc tcgctaaacg cggttgtata accgcaatac acgccgtatt ttatccggca
    25141 tatattgttt ttgtttgctc atggaatagg gaggttgtca tgtatcaacg ctgttttgat
    25201 aatgccagtg aaacgctgtt tgtcgccggt aaaacgccac ggctttcacg tttcgcattt
    25261 agcgatgatc caaaatggga gtctggacat cacgttcatg acaatgaaac cgagctgatt
    25321 tacgtcaaga aaggggtagc aaggtttacc atcgattctt cgttatatgt cgcgcatgcc
    25381 gatgacattg tggtgataga acgtggcagg ctgcatgcgg tggcctctga cgttaacgat
    25441 ccggcaacga cgtgtacctg tgcgctgtac ggctttcagt ttcagggggt tgaggaaaat
    25501 cagctactgc aaccgcattc ttgcccggta attgccgcag ggcagggaaa agaagtcatt
    25561 aaaaccttat ttaatgagct aagtgtgatt ttgccgcaaa gtaaaaatag ccaaacatct
    25621 tcgttatggg acgcatttgc ctatacgtta gcaattcttt actacgaaaa ctttaaaaat
//
LOCUS       DAZKHK010000080        25074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKHK010000080 DAZKHK010000000
VERSION     DAZKHK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.080"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            405..686
                     /gene="drpB"
                     /locus_tag="ACHOS5_003181"
     CDS             405..686
                     /gene="drpB"
                     /locus_tag="ACHOS5_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP9777952.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            726..1421
                     /gene="yedJ"
                     /locus_tag="ACHOS5_003182"
     CDS             726..1421
                     /gene="yedJ"
                     /locus_tag="ACHOS5_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP9777953.1"
                     /translation="MELQHWQAQFDNWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVTLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            1488..2906
                     /gene="dcm"
                     /locus_tag="ACHOS5_003183"
     CDS             1488..2906
                     /gene="dcm"
                     /locus_tag="ACHOS5_003183"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP9777954.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2887..3357
                     /gene="vsr"
                     /locus_tag="ACHOS5_003184"
     CDS             2887..3357
                     /gene="vsr"
                     /locus_tag="ACHOS5_003184"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP9777955.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGRKKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3346..4266)
                     /gene="yedA"
                     /locus_tag="ACHOS5_003185"
     CDS             complement(3346..4266)
                     /gene="yedA"
                     /locus_tag="ACHOS5_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP9777956.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4439..5356
                     /gene="yedI"
                     /locus_tag="ACHOS5_003186"
     CDS             4439..5356
                     /gene="yedI"
                     /locus_tag="ACHOS5_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP9777957.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5435..5617
                     /gene="yodC"
                     /locus_tag="ACHOS5_003187"
     CDS             5435..5617
                     /gene="yodC"
                     /locus_tag="ACHOS5_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP9777958.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            5788..7482
                     /gene="dgcQ"
                     /locus_tag="ACHOS5_003188"
     CDS             5788..7482
                     /gene="dgcQ"
                     /locus_tag="ACHOS5_003188"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP9777959.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTSMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKACPLAKLCHTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLHAQDIAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7479..8294)
                     /gene="yedP"
                     /locus_tag="ACHOS5_003189"
     CDS             complement(7479..8294)
                     /gene="yedP"
                     /locus_tag="ACHOS5_003189"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP9777960.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8590..8817)
                     /gene="yodD"
                     /locus_tag="ACHOS5_003190"
     CDS             complement(8590..8817)
                     /gene="yodD"
                     /locus_tag="ACHOS5_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP9777961.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8980..9168
                     /gene="dsrB"
                     /locus_tag="ACHOS5_003191"
     CDS             8980..9168
                     /gene="dsrB"
                     /locus_tag="ACHOS5_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP9777962.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9212..9835)
                     /gene="rcsA"
                     /locus_tag="ACHOS5_003192"
     CDS             complement(9212..9835)
                     /gene="rcsA"
                     /locus_tag="ACHOS5_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9777963.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKDTTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10125..10910)
                     /gene="fliR"
                     /locus_tag="ACHOS5_003193"
     CDS             complement(10125..10910)
                     /gene="fliR"
                     /locus_tag="ACHOS5_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9777964.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            complement(10919..11188)
                     /gene="fliQ"
                     /locus_tag="ACHOS5_003194"
     CDS             complement(10919..11188)
                     /gene="fliQ"
                     /locus_tag="ACHOS5_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP9777965.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11198..11935)
                     /gene="fliP"
                     /locus_tag="ACHOS5_003195"
     CDS             complement(11198..11935)
                     /gene="fliP"
                     /locus_tag="ACHOS5_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9777966.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(11935..12300)
                     /gene="fliO"
                     /locus_tag="ACHOS5_003196"
     CDS             complement(11935..12300)
                     /gene="fliO"
                     /locus_tag="ACHOS5_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP9777967.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEV
                     PQTDFQSVMKNLLKRGGRS"
     gene            complement(12303..12716)
                     /gene="fliN"
                     /locus_tag="ACHOS5_003197"
     CDS             complement(12303..12716)
                     /gene="fliN"
                     /locus_tag="ACHOS5_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP9777968.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(12713..13717)
                     /gene="fliM"
                     /locus_tag="ACHOS5_003198"
     CDS             complement(12713..13717)
                     /gene="fliM"
                     /locus_tag="ACHOS5_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP9777969.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(13722..14186)
                     /gene="fliL"
                     /locus_tag="ACHOS5_003199"
     CDS             complement(13722..14186)
                     /gene="fliL"
                     /locus_tag="ACHOS5_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP9777970.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14291..15418)
                     /gene="fliK"
                     /locus_tag="ACHOS5_003200"
     CDS             complement(14291..15418)
                     /gene="fliK"
                     /locus_tag="ACHOS5_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9777971.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFGGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15415..15858)
                     /gene="fliJ"
                     /locus_tag="ACHOS5_003201"
     CDS             complement(15415..15858)
                     /gene="fliJ"
                     /locus_tag="ACHOS5_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9777972.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(15877..17250)
                     /gene="fliI"
                     /locus_tag="ACHOS5_003202"
     CDS             complement(15877..17250)
                     /gene="fliI"
                     /locus_tag="ACHOS5_003202"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9777973.1"
                     /translation="MTTRLTRWLTTLDTFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLESYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(17250..17936)
                     /gene="fliH"
                     /locus_tag="ACHOS5_003203"
     CDS             complement(17250..17936)
                     /gene="fliH"
                     /locus_tag="ACHOS5_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9777974.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(17929..18924)
                     /gene="fliG"
                     /locus_tag="ACHOS5_003204"
     CDS             complement(17929..18924)
                     /gene="fliG"
                     /locus_tag="ACHOS5_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9777975.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(18917..20575)
                     /gene="fliF"
                     /locus_tag="ACHOS5_003205"
     CDS             complement(18917..20575)
                     /gene="fliF"
                     /locus_tag="ACHOS5_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP9777976.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            20790..21104
                     /gene="fliE"
                     /locus_tag="ACHOS5_003206"
     CDS             20790..21104
                     /gene="fliE"
                     /locus_tag="ACHOS5_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9777977.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            21449..21781
                     /gene="emrE"
                     /locus_tag="ACHOS5_003207"
     CDS             21449..21781
                     /gene="emrE"
                     /locus_tag="ACHOS5_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP9777978.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            21950..22501
                     /gene="ybcL"
                     /locus_tag="ACHOS5_003208"
     CDS             21950..22501
                     /gene="ybcL"
                     /locus_tag="ACHOS5_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFP9777979.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            22511..23308
                     /gene="ybcM"
                     /locus_tag="ACHOS5_003209"
     CDS             22511..23308
                     /gene="ybcM"
                     /locus_tag="ACHOS5_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9777980.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNKQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(23810..24892)
                     /locus_tag="ACHOS5_003210"
     CDS             complement(23810..24892)
                     /locus_tag="ACHOS5_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFP9777981.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT         6162 a         6218 c         6427 g         6267 t
ORIGIN      
        1 tagggagtat caatgattgt tgcgctaaac ggctggtgtg atctcgcgca cattaatgaa
       61 ggcataaaaa aaagccggca tcatgccggc ccaaaaaata taaacaagac gataaaaggc
      121 taataacgga ggcatgatga cacagaaaaa taaaaaggta taattgtaat cagtaccaat
      181 atattgattg aatgaattaa taaagccgaa atcaaagata aataacatcg ttcaaatcga
      241 tttttcctgt attcaccgcc gttgcgcaaa atttatctat ttgttcaaaa aatgattgag
      301 tcttgactgg ctcatccaat gtggaaaaat gtgactttta tcacataata gttctaagtc
      361 tgaattttcc gggttatctc aaaatggaat acggttcgac aaagatggaa gagagactct
      421 ctcgcagccc tggcggcaaa ttggctttgt gggctttcta tacatggtgt ggctatttcg
      481 tctgggcaat ggcgcgatac atctgggtga tgagccggat ccccgatgcc cccgtgtcgg
      541 gatttgaaag tgatttagga tctaccgcgg ggaagtggtt aggagcattg gtcggatttc
      601 tgtttatggc cctggttggt gccttgttag gcggcattgc atggtacacc cgacctcgtc
      661 ccgcacgttg cagacgctat gaataaaaac tgctaaggtt gcgactcttt ggcaacggag
      721 cgcgtatgga gttacaacac tggcaggcac agttcgataa ctggttgaaa aaccatcatc
      781 aacatcagga cgcggcgcat gacttgtgtc attttcgccg tgtctgggct acggcacaga
      841 aactggcggc agatgacgat gtcgatatgc tggtgatttt aaccgcctgt tattttcacg
      901 atatcgtgac cctggcgaaa aatcatccgc aacggcagcg ttcttcaatc ctggcggcag
      961 aagagacgcg tcgcctgctc cgtgaagagt tcgcgcaatt tccggcggag aaaattgagg
     1021 ccgtttgtca tgccattgcc gctcacagtt tcagcgcgca aatcgccccc ttaacaacgg
     1081 aagctaaaat cgtccaggat gcggatcggc tggaagccct gggggcgatt ggcctggcgc
     1141 gcgtatttgc cgtttcaggg gcattgggct tggcgctgtt tgatggcgaa gatccgttcg
     1201 cacagcatcg cccgcttgat gataaacgct acgcgctgga tcatttccag acaaagttac
     1261 tgaaattacc gcaaaccatg caaaccgcaa gaggcaagca gctggcacag cacaacgcgc
     1321 aatttttagt cgagtttatg gcgaagctcg gtgccgaact ggcgggggag aatgaaggta
     1381 tcgatcacaa ggtgatagat gcgttttcac ctgctggctg agcgcgtggc cctaaacggc
     1441 agtaattatg ttaacctgtc ggccatctca gatggccggt taaatctatg caggaaaata
     1501 tatcagtaac cgattcatac agcaccggga atgccgcaca ggcaatgctg gagaaactgc
     1561 tgcaaattta tgatgttaaa acgctggtgg cgcagcttaa tggtgtgggt gagaatcact
     1621 ggagcgcggc aattttaaaa cgtgcgctgg cgaatgactc tgcatggcac cgtttaagtg
     1681 agaaagagtt tgcccatctg caaacgttgt tacccaaacc accggaacat catccgcatt
     1741 atgcgtttcg ctttatcgat ctatttgccg gaattggcgg catccgtcgc ggttttgaat
     1801 cgattggcgg acagtgcgtg tttaccagcg aatggaacaa acatgcggta cgcacttata
     1861 aagccaacca ttattgcgac ccggcgacgc atcattttaa tgaagatatc cgcgacatca
     1921 ccctcagcca taaagaaggc gtgagtgatg aggcggcggc ggaacatatt cgtcaacaca
     1981 ttcctgaaca cgatgtatta ctggcgggtt ttccttgtca gccattttcg ctggctggcg
     2041 tatcgaaaaa gaactcgctc gggcgggcgc acggttttgc ctgcgatacc cagggcacgc
     2101 tgttctttga tgtggtgcgc attatcgacg cgcgtcgtcc ggcgatgttt gtgctcgaaa
     2161 acgtcaaaaa cctgaagagt cacgataagg gaaaaacgtt ccgcatcatc atgcagacgc
     2221 tggacgaact gggctatgac gtggctgatg cagaagataa cgggccggac gatccgaaaa
     2281 tcatcgatgg taaacatttc ctgccgcagc accgtgaacg catcgtgctg gtgggttttc
     2341 gtcgcgatct gaatctgaaa gccgatttta ctctgcgtga tatcagcgaa tgtttccctg
     2401 cacagcgagt gacgctggcg cagctgctgg acccgatggt tgaggcgaaa tatatcctga
     2461 cgccggtgct gtggaagtac ctctatcgat atgcgaaaaa acatcaggcg cgcggtaacg
     2521 gcttcggtta tggaatggtt tatccgaaca atccgcaaag cgtcacccgt acgctgtctg
     2581 ctcgttatta caaagatggc gcggaaattt taatcgaccg cggctgggat atggccacgg
     2641 gtgagaaaga ctttgacgat ccgctgaatc agcaacatcg tccacgtcgg ttaacacctc
     2701 gtgaatgcgc gcgcttaatg ggttttgaag cgccgggaga agcgaaattc cgcattccgg
     2761 tttcggacac tcaggcctat cgccagttcg gtaactcggt ggtcgtgccg gtctttgccg
     2821 cggtggcaaa actgcttgag ccaaaaatca aacaggcggt ggcgttgcgt cagcaagagg
     2881 cacaacatgg tcgacgttca cgataaggcc acccgcagca aaaatatgcg cgcgattgcc
     2941 acgcgtgata cggcgattga gaagcgcctc gccagtttgt taaccgggca gggccttgcg
     3001 tttcgcgttc aggacgccag tctgcccgga cgcccggatt ttgtcgttga tgaatatcgc
     3061 tgcgtgatat ttacccacgg ctgcttctgg catcatcatc actgctatct gtttaaagtg
     3121 cctgcgaccc gaaccgagtt ctggctggag aagataggta aaaatgttga acgcgatcgc
     3181 cgcgatatca gtcgcttgca ggagctcggc tggcgcgtac tgattgtctg ggagtgtgcg
     3241 ttacgtgggc gcaagaagct gacggatgcg gcgcttaccg agcgtctgga agagtggatc
     3301 tgcggcgaag gtgccagcgc gcagatcgac acgcagggga ttcatttact cgcttgatgc
     3361 atcctgaata actggcgcga caacgggttt ggccgggaag agatattttc ccaacgtgac
     3421 cagtaccacc gcgaaaacaa ttacgccgag cgccagccat tcaattttcg acagcgtttc
     3481 gccacccagt cccgtaccca gcaagaccgc gaccaccggg ttaacgtagg cgtagctggt
     3541 ggcgagagcc ggactgacat tacggattaa atacatataa gcgttaatgg cgataatcga
     3601 accaaacagc gccagatagc cgaccgcaag gaagcctgac agggaaggga gcgccgtcag
     3661 tttttcaccc gcaatcatcg atgcgatcat taacaccacg cctgccgcca gcatctcaat
     3721 cgcacccgcc atcatcccta caggtaaggt aatgcgcgag ccataaactg agccaaacgc
     3781 ccagctaatc gagccgatta aaatcagaat cgcgccccac ggattgccgc ttaaatttcc
     3841 accgctattg agcatgatga ttccggcaag cccaatggcg atacccaccc attccagttt
     3901 gcgcgtttta atgccaaaca ggcgactgaa gcacagggta aagaggggca cggttgcaac
     3961 cactacggcg gcgatgccgg aaggaacatt ttgatgttcg gcaaccgtca ccatgccatt
     4021 accgacagcc agcaataaca ggccaatcag cgcggcattg agcagcggac gtagcggggg
     4081 aagtttgtgt ccgcgcagta gcaaaaatgc cagcaataaa ataccggctg ccaggaatcg
     4141 aacgcccgcc atcattaacg gcggccagct ttccacgcca atccgaatga caaaataggt
     4201 tgagccccaa atgatataca acgcaaacag cgcgccaaaa agcggtaaca actggcggaa
     4261 acgcataatc cctcacggcg gaaataaaaa ggtggttcat agtaaacgtg aaaatcattc
     4321 tgctggcgag agatataatt gcacttgatt gttaaataaa tgttgacctg tgaagcaagt
     4381 atcagagctg ccgtttttgc ttcatactta cacccttcaa caataaaaat gagagggaat
     4441 gcttttggcc gggagtagtt tactgacgtt gctcgatgat atcgccacac tgctggacga
     4501 tatctccgtg atgggcaaac tggcggcgaa gaaaaccgcc ggtgtattag gggatgattt
     4561 atcgctcaat gcgcaacaag tttcaggcgt gcgggccaac cgggaacttc ccgtggtctg
     4621 gggcgtggcg aaaggatcgc tgatcaataa agtgattctg gtgccgctgg cgttgatcat
     4681 cagtgcgttt atcccgtggg cgattacgcc actgttgatg attggtggcg cgtttctctg
     4741 ctttgaaggg gtagagaaag tgctgcatat gctggaggcg cgtaaacata aagaagatcc
     4801 agcgcagagc cagcagcgtc tggagaaact ggcggcgcag gatccgctga agtttgaaaa
     4861 ggacaaaatt aaaggggcga ttcgtaccga ttttattttg tctgcggaaa tcgtcgccat
     4921 tacgctgggg attgtggcag aggcaccgtt gcttaatcag gtgctggtgc tttcaggcat
     4981 cgcgctggta gtgaccgtgg gcgtctacgg tctggtaggg gttatcgtta agattgatga
     5041 cctggggtat tggctggcgg aaaaatccag cgcgctgatg caggcattag gtaaagggtt
     5101 attgattatc gcgccctggc tgatgaaagc attatcgatt gtcggcacgc tggcgatgtt
     5161 cctcgtcggc ggcgggattg tggtacatgg tattgcgccg ctgcatcacg ctattgaaca
     5221 tatcgccggg cagcaaagtg cagtggtggc gatgatatta ccgactgttt taaatctgat
     5281 tcttggattt atcatcggcg gcatcgtggt gctgggagtg aaagcagtag cgaaaatgcg
     5341 cggtcaggca cattaatact cgcactggct attatgcaaa attcgccatc ttcaactaag
     5401 gtgaacaagt ttcactcgaa aaaaggaggc gagtatgagc tttatggtta gtgaggaagt
     5461 tacggttaaa gagggcggcc cgcggatgat tgtcactgga tactccagcg gtatggttga
     5521 gtgtcgttgg tatgacggtt acggggtcaa gcgggaagct tttcatgaaa ccgatcttgt
     5581 tccgggggag gggagtcgtt ctgcggaaga agtttgaaag ggtatcatta acaccaggcc
     5641 ggatcacatc cggcctggtg tcccaatgcc tgcaatgaat gatagtcgcg tcttatcatg
     5701 ccaccattat aaaattatcg ctgttttgcg gtacgctatc gccgggttat gccagaatca
     5761 taaaaaagca ggttgggagt cgtcagggtg cagcacgaga caaaaatgga aaaccagagc
     5821 tggttgaaaa aactcgcacg ccgcctgggg cccggtcatg tcgttaatct ctgctttatc
     5881 gtggtattgc ttttttccac cttgctcacc tggcgtgaag tggtggtgct ggaagatgcc
     5941 tatatctcca gccagcgtaa tcatctggaa aacgtagcca acgcgctcga taagcatttg
     6001 cagtataacg tcgacaaact gatctttttg cgtaatggca tgcgcgaagc tctcgtggcg
     6061 ccactggatt tcacctctct gcgtaatgcc gtaaccgagt tcgaacagca tcgcgacgag
     6121 cacgcctggc aaatcgaact caaccgacgg cgcaccctgc cagtcaatgg cgtatcggat
     6181 gcattagtca gcgaggggaa tctcctgtct cgcgaaaatg aaagcctcga caatgaaatt
     6241 accgctgcac tggaagttgg ttacttgctg cgactggcgc acaactcctc gtcgatggtt
     6301 gaacaggcgg tgtatgtctc gcgtgctgga ttttacgttt cgacgctgcc gaccttgttt
     6361 acgcgcaatg taccaacgcg ttattacggc tatgtcaccc aaccctggtt tatcggccat
     6421 tcgcaacgag aaaatcgtca ccgcgcggta cgctggttca cttcgcaacc ggaacacgcc
     6481 agcaatactg aaccgcaggt taccgtcagt gttccggtag acagtaataa ctactggtat
     6541 ggcgtgctgg ggatgagtat tcccgtgcgt actatgcagc aatttttaag aaacgccatc
     6601 gataaaaacc tcgatggtga gtatcagctc tatgacagta agctgagatt tttgacctct
     6661 tccaatcctg accatccaac agggaatatt tttgatcctc gtgaactggc cttgctggcg
     6721 caggcaatgg aacatgacac gcggggcggc attcgtatgg acagtcgcta tgttagctgg
     6781 gaacgtctgg accatttcga cggtgtgctg gtgcgcgtcc atacgctaag cgaaggcgtg
     6841 cgcggcgatt tcggcagtat cagcattgca ttaaccctgc tgtgggcgct ctttaccagc
     6901 atgttactcc tctcctggta tgtgattcgc cggatggtca gcaacatgta tgttctgcaa
     6961 agctcgttgc agtggcaggc gtggcacgac accttaacgc gtttatataa ccgtggcgca
     7021 ttgttcgaaa aagcctgtcc gctcgctaaa ttgtgtcaca cgcaccaaca tcctttttct
     7081 gtcattcagg tcgatcttga ccattttaaa gcgattaatg accgctttgg tcatcaggcg
     7141 ggcgaccgtg ttctttctca tgctgccgga ttaattagca gttccttaca tgcgcaggac
     7201 attgccgggc gggtcggtgg tgaggagttt tgtgtgattc tgccaggcgc gaatctgacg
     7261 caggctgcgg aggtcgcaga acgtattcgc ctgaagttaa atgaaaaaga gatgctgatc
     7321 gccaagagta caacgatacg catcagtgcc tcgctggggg taagtagcag cgaggaaacc
     7381 ggtgattatg attttgaaca actccagtca ttggccgacc gtcggcttta cctcgcaaaa
     7441 caggccgggc gtaatcgggt atttgcgagc gataacgctt aacgggcgga gaaaaaatgg
     7501 tccagccctt cacgccagcc ttccggtcct tcacgctgcg ttcgccagac gcgggccgga
     7561 tcctcatcat gcagatggac cccttcacgg tttagccctt tcacaatcac cgcgtaatcc
     7621 attacctcca gtaaaggcgc atcgtttggc ccatcgccca ggccaagtgt ggttgggcgt
     7681 ctgcctgaca attgttgata ggtcgcgata atccagttgg cagcctgatc ttttccggca
     7741 gaggcatcca gtacgtgcca gaagcgcgca ccctgcataa actgcaagcc cagttcgttc
     7801 agacgagcga taaattgtgc catacgctcg tcactgtcgc gccagattag cgttaccgac
     7861 gcctcatgca gctgcgtcag cgccgcctgg ctacggctta atcccgtcca ttcggcaatg
     7921 gttgcatcgt cgacatcatc aaaagtcgtg aatttaaaat gttctttctc gcgtagcgta
     7981 tttaaaacct ggcagatttc gccatggcta atacctgaga tgatgcgtgg aaaaccgtct
     8041 atatcctgcc attgttcagc aagctggatc actgcgccat tctctgcaat cagcggtaag
     8101 ccttgtagcc ccaacatttt ttgcaagtac agcatttccg ctgaagtttt actgctacag
     8161 agaatgacgg gaatattggc ttcgtgtaaa cggctgagcc agggggctgc cggttgccag
     8221 tcataactat gactgtccag cagggtgcca tcaagatcgc taaaaaccag tagtggttgt
     8281 tgaattgaaa acatgggcgc agtactcccc ttaccaggat gattctcaaa acagcacaaa
     8341 taatcaaaga tgattttttc gggagcgatc ccgttgacac cagagccacc aaggggtgac
     8401 gcgcgtcaca tttctattca taagtagcgt taatcattca tagcgcgaat attttcttgt
     8461 cagcgaaaaa aattgcagat aaggtgatga acacatcaga tttcctggtg taacgaattt
     8521 tttaagtgct tcttgcttaa gcaagtttca tcccgacccc ctcagggtcg ggattttttt
     8581 attgtgcatt caacgattca cttcagagac gctgaagtca tgaatatcca gttcgaaggc
     8641 atccgccaat tcacgccatg tatgatattc acgtccatcg acgctaacgt gttcagaatc
     8701 gttctggctg cgatgaactt cgctttcgct aatttcattg atcgccgcca gcagggcatc
     8761 gacatcgacg ctgacctcac gttttgcggt atcgctgtac tcttttgcgg ttttcatcat
     8821 ctttcctcgc aaccgttact ctgttgttgc cgccagtcgt taagtccgcc tggcatctct
     8881 taagtataga ccgtcgagaa aatcagcact cgcgcgcgcc tggcgctgcc agaaaaacga
     8941 aattgttcta cactggcaca aagccacagg aggaaaacga tgaaggtgaa tgatcgggta
     9001 acagtcaaaa cggatggcgg tccgcgtcgt cctggcgtgg tactggcagt tgaggagttt
     9061 agtgaaggca caatgtacct ggtttcgctg gaagactacc cgctcggcat ctggttcttt
     9121 aatgaagcag ggcatcagga cggtatcttt gttgagaaag cagagtaatc ttattcagcc
     9181 tgactggtgg gaaaccacca gtcagaatgt gttagcgcat gttgacaaaa ataccattag
     9241 tcacattatc cgtcagtcgg acgacatggt agataacctg tttattatgc gttttgatct
     9301 tacgtttaat attaccttta tgcgatgaaa cggtcttggc tttgatattc atttggtcgg
     9361 agatttgaat ggttccctga cctgccatcc acattcgcaa catactcgat tcggttcggc
     9421 tcaatgataa agtcggcata tttaaaaacg aggttatcgt tgtatctttt ttcagaatat
     9481 cgccaaggat atcgtcgaga gattccggtt taatcgattt agagctgatc aataaatttt
     9541 ttctgaccaa tagatattca tcaaaatgaa cattggcaat tgccataaaa acgataaata
     9601 acgtattggg atgttgatta atgatgtgct tgatgcgctg actgttagaa gcatcgtgga
     9661 tgaaacagtc ctcattaata aacaccactg aagggcgctg tgaatcacaa gctatggcaa
     9721 ggtcatcaac ggtttcaatg tcgttgattt ctcttttttt aacccctcta ctcaacagat
     9781 acccggttaa acctagtcgg gtgtaactac ataaatccat aataatcgtt gacatggcat
     9841 accctcactc aatgcgtaac gataattccc cttacctgaa tatttcatca tgactaaacg
     9901 gaacaacatg ggtcacctaa tgcgccactc tcgcaatttt tcaggcggac ttactatccc
     9961 gtaaagtgtt gtataatttg cctggaattg tcttaaagta aagtaaatgt tgcgatatgt
    10021 gagtgagctt aaaacaaata tttcgctaca ggagtatcct ggaagatgtt cgcagatatt
    10081 atttttcgga taatccttag ggtagcatga taaacgttac ggaattatat taatggtaat
    10141 tcactaataa tatcagccag caaattaaaa atttcactga ataaatgttc gcaaaaaggc
    10201 gcaattaacg gcattaatgc cgccattaaa gagatgccga cagtcagagt taatggaaat
    10261 ccaataacaa aaatggataa ttgcggggcc atacgattca gtaaacctaa tgccagattc
    10321 agggtcagca acagagtaat gagcggtaac gccagcatca gcccgttaag gaaaatcaaa
    10381 ctccctgctt tggtaagtgc cagaaacgca ttgctgttca acggttcacc accaatcggc
    10441 agggtgtgaa aggtatcgac cagcagtgaa atcaaccata aatgaccgtt aaatgtcagg
    10501 aacagcagta atgccagcat atccatgatg cgcgctaaaa cgggcatatt gagatggctg
    10561 ccaggatcga caaatgtcgc aaatgacagc cccatttgca gaccgataat ttcgccagcg
    10621 gttcgcacag cggcaaaggc aaattgcatg gtaaaaccaa gcgcaatgcc gatcaggatc
    10681 tgctgcacgg ccagccacag agcaaagaac gaaaaaacag gaacatcgtt ggcaggtaat
    10741 gatggggcaa tggcgaacgt gatcatcatt gccagaccca gttttacccg tttcggcacg
    10801 ctgcgttcgc tcagaattgg cgcggtagaa atgagcgcca gcacgcgcag taacggccag
    10861 aagtacaggc ttaaccagga aagccattga tcgcttgtca cctgcatcat tgtacgggct
    10921 acccgatgat atacggcagg ttagtgaaca aggtgcggac gtaatccagc aacagattga
    10981 gcatccacgg tccggcaata ataatggcga taaatacggc gatgattttc ggaataaacg
    11041 acagcgtcat ttcgttaatc tgcgtggcgg cctgcaaaat actgatgata aggcccgtga
    11101 ccagcgccac cagcaacagc ggggctgcca gtgccagcgc gactttcatc gcttcagttc
    11161 ccatcatcat gaccgattca ggtgtcattt tgcctctcta gctgtaaaag ctctgcgcca
    11221 gcgaaccgac cagcaattgc cagccatcca ccagtacaaa cagcatcagt ttaaagggca
    11281 gagcaattgt ggctggggga accatcatca tccccaatgc catcaacacg ctggcaatca
    11341 ccaggtcgat aatcaaaaaa gggatgaaaa tcgtgaagcc tatctgaaat gcggttttca
    11401 actcgctggt cacgtaggcc gggagcaaaa tgcgcatcgg cacagcttca ggtccctgca
    11461 acgggccggt attcgccagt ctggcaaaca accctaaatc tgcctcacgg gtctgacgca
    11521 gcataaactc acgcagcggc tgcgcccctt tttccagcgc ctcctgcatt gatattttct
    11581 cttcgctgaa tggctggtac gcatcaacat aaattttgtc gatcaccggc gacataataa
    11641 aaaaggtcaa aaacagtgcc agccccagca atacctggtt aggtggcgcg gagggcgttc
    11701 ccagcgcgtt acgcaataaa ccaaaaacaa taatgatgcg ggtgaaactg gtcatcatca
    11761 gtaaaatcgc tggaatgaac gtcaacgagg tgatgaacac cagcgtctgc accgggagcg
    11821 accagctttg tccaccgcca ggtagcggct ggctggtgat acccggcagt tgcgcgaagg
    11881 cgaggggcgt aatcagccag agaaggaccg gtgcgacaga aaacaaacga cgcatcagga
    11941 tctcccgcca cgcttaagca aatttttcat gaccgactga aaatcggttt gcggtacctc
    12001 ttccgttggt gctgaagggg gaagtttatg cagcagattg atttgccctg cggtaacgcc
    12061 gagcaccagc cgtgcatctt ccacatcgac caccacgacc cgttcacgcg cgcccagcga
    12121 agcactggcg ctaattttca gaccgttaac gccagtgcgt ttaggggcaa atcccagccg
    12181 ttttaccagc caggcagcag cgaggatcag ggcaataatg gcgatgagtg cgccgctcac
    12241 ctgcagcagt ggctcagcag aaaccggcgc ggaagattgc acagtagcat ggttattcat
    12301 cactaacggc tcaggcggcg catccgctcg gacggggtaa tgatatcggt gatccgcacg
    12361 ccatatttat cggcaacaac caccacttcg ccctgggcga ttaaataacc gttgatcaga
    12421 atatccagcg gttcgcccgc cagaccgtcc agcgccacga ctgacccttg cgtcagacgc
    12481 aacagctctt tgatggtcat ccgcgtacgg cccagctcga cggtcagctt gaccggaata
    12541 tccataatca ggtcgatatc ctgcaacgtt ccgctgacat caccaccgcc aaattgctgg
    12601 aacaccgcgt cggcagcgct ttttccgctg gttgattttt gctcgctcaa cgcttcagcc
    12661 cacagatcgt ccattgcgcc gttgttgtca tcggccggat tattcatgtc actcatttgg
    12721 gctgttcctc gttcagagaa tttaaaatcg ggttaatcaa atgttctatc cgtaacgcat
    12781 actgaccgtt gagggtgccg tactgactgg tcagtaccgg aacgccgtca acatgggcga
    12841 tgatgcgatc gggtttttct atcggcagga cgtcgccggg tttcagtttt aaaatctgcg
    12901 acaggcgtag cgagatatcg gcaaagttgg ccaccagttc cagctgtgaa tgctgcacct
    12961 ggcgcaccag gttatcgcgc cagttctgat cttcattacg cgagttttcc agcggcgggt
    13021 taaccaacaa ttcccgtagc ggctcgatca tgctgaatgg caggcagata ttaaattcgc
    13081 cggtcaggtt gccaatctcc acatggaacg gtgtgttaac cacgatgtca tttggtgagg
    13141 tggtgatatt ggtaaatttc acctgcattt ccgaacgcac gtactcaact tccagcggat
    13201 taatcgcctt ccaggcatcg ctatagcctt caagcgccag ctttaacatg cggttgatga
    13261 cgcgctgttc tgtatgggta aactcgcgac cttccacttt ggtcgggaag cgtccatcgc
    13321 cgccaaacag gttatccacg gcgataaaca ccaggctcgg cgagaacacc accagccctg
    13381 tgccgcgcag cggtttaagg tggataaggt tcaggttggt cggcaccggc aggttgcggg
    13441 caaattcatg gtacggctga atgcggatgg ccccgacggt aatgtccggg ctacggcgca
    13501 gcaggttgaa cagccccata cgaaaatggc gggcaaagcg ctcattaatg atttccagcg
    13561 cctgcaaacg ttcgcgcaca acccgtcgtt gggtattcgg atcgtacgga cgaatgtcac
    13621 tttcgccgct aacactggct gtcggttcgt ctttgacttc gctgtcacca ttcaacagcg
    13681 catcaatttc agcttgagaa agaatactat cgcccatgtc gttatcgcag aataaaagcg
    13741 gtatacagca cgtcggtgac atcctgtttc ggttgcccgg caacaagcgg ggtggaaagt
    13801 gtggttttaa tctcggcaat caggtttttc ttgccttctt ctgtcgccag tacggcagca
    13861 tcctgacgcg aaaacagtaa cagcaagcga ctacggactt ccggcaaata ctcactcagc
    13921 cgcgagcggg ttgcttcatc tttcagtcgc agggttatgc cgatataaag cacgcgatcc
    13981 gcatcgccca aattgaccgt aaaggtatcc agagcataga agaccgggga gggcacgacg
    14041 cgttgctgcg ctttgtcgtc agcggcaacc tgatgcgaat gccagtagct gtaacctgcg
    14101 ctggcacagg ccgcgagggt aatgaatacc agaatcggga tccaaagcga tcgcttgctt
    14161 ttcttgctta tcgcgtaatc agtcatgtgt tgcggtcttc ctgtgtcgct actgcttatc
    14221 ggctaattat cctgcgtctt gagcgcaaca aagcggggaa aagacgcgga ttacggtgct
    14281 acctctgacg ttaggcgaaa atatcaacgc cgctgttacc tgttacacgc ccttgtaaag
    14341 agacgggaac cggaagcgta tcgtcgtctt cccccgccag aggttcatgg tttgctgtgc
    14401 gttggctttg ctgttgctgg gaagcggcct gctgctgacc accaaagctt tcgccactga
    14461 tgttgctttg ccctaactga atgccacttt cggccagctg cgtacgcagt accggcagcg
    14521 ctgcttccag ggcggcgcgt acgtgctgat gcggtgaaat catctggatc tgcgcctggt
    14581 tatcatccac tttgagggaa atttgcactt cacctaaatc ctgtgggtgc agacgcaact
    14641 ctgcactttg ttgcccctgg cgggtgaaca gcgaaatatg ctggcttaat gattgttgcc
    14701 attcgtgaga acccagcggt gcactcaaaa caggcgcggc gacggtgggc agtggctgtg
    14761 tctggtgtgg agtgattagc gggctggcgt cagcggtcac cggcgaaggc gtgctgatga
    14821 cttccgcttt actctgggct tctgctacca gcggtgttaa tggctgagct ggtgtgccgg
    14881 gggcgtcatc aggctgtgct gtagtgagtt gcgcagaagt cagttttgtg aagagcgttg
    14941 gtttctctgc cggtaacacg gttgacggtg catcagtcac tttgggcgta ttgtcaaaac
    15001 ccggcaacat cgcaaaaagg gcgctcagac ttgcggtgac gtcttcatta aggtcatccg
    15061 ctttttcgtc ttttgtcgta tttttgtcag ccaccgcagc caacgtcatc gtttgagcgg
    15121 tggttaacgg tgttgatgtg gattgttcgt cgttgatgac aggaagtgtt tcatccacag
    15181 gaatcagtaa atccgcttgt tgcgcatcgg aaacaatatc gctgaccagc ggttcgcctt
    15241 ttgtcgtggg cttatctgtt gccaccagca actggggggc cgctttgtcg gttgttgtct
    15301 cgcctgctaa tgcttcgctc aacaacgcga gaaaatcttg tgcagcatcg ctggctttgc
    15361 cgccaggcaa tgtggtggtg tcaacgtcgg cggtaatcaa gggcgctaag cgaatcattc
    15421 aggtttcctc atggcggcgc gctgggcgaa ctcatccatc tttttctgat cgaggcggtt
    15481 ttctgcaagc agtgccgccg tggattgccg ttcctgcagt gtctgccagg cctgcaaacg
    15541 ttgtttcttt tctcgccagc tgttcagggc aatgtcaact ttctgcgtcc actgattaag
    15601 ttgctggcga tgctgagtaa tggctttttc cagcgtctgg ataaactgct gatagttgat
    15661 ccagcggttg ctagtcatcc cggcactcat atcgctattg aggttattgc gatattcatt
    15721 ctgataatcg atcagcattt tgagctgttc ttccgcctgc tgacatccgc gacgcatttc
    15781 acccagcagg cgagcggcat cctctacctc tttttctgcc agatctttca gggtcgccag
    15841 cgcaccatgt tccgccatct gccgttatct cctgggttat gacactgtcg ggaaaatacg
    15901 ctccagcccc tggagagacg cttcccagtc cgcgcgttca aaaatgcctt gttgcaaata
    15961 gctctccagc tgcggccaca gggcgatggc tttgtcgagc atcggatcgc tgcctttggc
    16021 atacgcgccg acgctaacca gatcgcggtt acgctgaaaa ctcgacaaca gctgtttgaa
    16081 ggtgcgcact cgcgcgtaat gttgctcact gatcaacgcc gtcattgcgc gactgatcga
    16141 cgcttcaata tcgatagccg gatagtgccc ggcttccgcc agtcggcgag acagcacaat
    16201 gtgaccgtcg aggattgccc gcgcggagtc agcaatgggg tcctgctggt catcgccttc
    16261 ggtgagcacg gtataaaacg cggtaatcga gccgccgccg ctaataccat ttccggcacg
    16321 ctcgaccagt gccggtaatt tggcaaacac cgacggtgga taacctttag tggcaggtgg
    16381 ttcgccaatc gccagcgcaa tttcacgctg ggccatcgcg tagcgggtga gggagtccat
    16441 aatcagcaac acatgctgac cacgatcgcg aaaatcttcg gcaatgcgcg tggcataggc
    16501 ggcaccctgc attcgcagga gcggagaaac atccgccgga gcggcaatca ccactgagcg
    16561 tgcacgtcct tcggcaccga ggatattctc aataaaatct tttacttcgc gcccacgttc
    16621 accaatcaaa cccacgacaa tgacatcggc gcgggtgtaa cgtgccatca tccccagcag
    16681 cacacttttc ccaacgccgg acccggcaaa cagccccata cgctgcccgc gtccgacggt
    16741 aagcagggca ttgattgggc gcacgccggt gtccagcaca tgttcaatcg gtgtacgttg
    16801 caacgggtta aatggcgggg taatcagcgc accggtttcc gtcgtatcgg ggaagggcag
    16861 gccatcgagc ggtttaccgc taccgtccag aacacgacct aataacgccg gaccgagcgg
    16921 caactgcttg ccgctttgca gcccttctgc cgaaatgttt ttggcatata cacgcgcgcc
    16981 gggcaggaca ccttccactt cctccagcgg cattaaaaac agccgttgac cgttaaagcc
    17041 aacgacttcg ctttctactt cgtgcgtttc gctgccgttc tggcgctcaa tgacacaggt
    17101 tgcgccgagc ggcaattgta atccggtggc ttccagcacc agcccggtag cgcgggttaa
    17161 tcgcccgtag cgacgtaccg caggcaactg cgccattttg gcttcaaagg tatccagcgt
    17221 ggttagccag cgagtcaggc gcgtggtcat tacaccactc ctggtgctgc cagacggcag
    17281 agttcttgcc agcgggtggc gacactggcg tcgagatcgc cttcatcggc ggagacttta
    17341 cagccgccag gatggagggt gggatcgccc cgcaagcgcc agccatgcaa acttaaggtt
    17401 gcgccgagca tatcatccac acgttgcaga tcatccgggt gcacgcgcag ctgtggttta
    17461 ccgctgaata acggttcttg ctgcaacaac tgttggatct gtttgatcag tgccgagtta
    17521 tccactgttg gcgtctgacc gatcacctga cgtgccgcct ccagcgccat ctgcatcagg
    17581 cgcgacgcta tcacactatc aagtgcatca agggtagttt gaaattcgct gaccagttgc
    17641 tgcatccggg catgaattgg cgcttgttga gacttcgcct ctgccagacc ttgctccagc
    17701 ccctgggcca gtccttcctg atagccctgt tcgtgacctt gctggcgacc ttcggcaata
    17761 cccgcctgat aaccctgctc gtgggcctgc atttgcagtt gcgccagttg ctgctcaagg
    17821 ctgggttcgg cctcttcaat aatggtttct tccgactcga ccattggcac aaactctgcc
    17881 ggtggtggcg cgagatcgtc cggcgtccag gttttccacg gcagattatc agacataggt
    17941 atcctcgccg ctgccaatca ccatctcgcc agtttcggca agacggcgca caatcagcag
    18001 aatcgctttc tgttcgtttt ccacctgcga cagacgcacc ggaccacggt tggcgagatc
    18061 gtcgcgcaga atatcggcgg cacgctgcga catattgcgc aggaatttct cgcgcagtgg
    18121 ctgctcggcc cctttcagcg cgatcagcag cgattcggaa tccacttcct gcaacagacg
    18181 ctgaatgctg cgatcatcga catccaccag attctcgaac aggaacatct cgtcgatgat
    18241 tttctgcgcc agctcgccgt cgaattcacg cacggcggta ataacggctt cttcctgctg
    18301 cgttttcatc aggttgataa tttcggctgc cgttctcacg ccgcccattt tgctgcgctt
    18361 gagattctga ccgtcgagca agccattcag tacttcggtc agttccgcca gcgcggctgg
    18421 ctgcacgccg ccaaaggtgg cgatacgcaa catcacgtcg tggcgcagac gttcatcgaa
    18481 caacgccaga atatcggcgg cctgggcgcg cttcagatgc accagaatgg tggcgataat
    18541 ttgcggatgc tcatcgcgaa tcagatcggc ggcgctctgc ggctccataa agttgagcgt
    18601 ttcaataccg ctggcggtat cgcgggtttc gagaatatct tccagcaggc tggcggcacg
    18661 ttcttcaccc agagctttga ccaataccga acgcagataa tcgttggcgt tgatattcag
    18721 tgcagcaaac tgttcagctt cttgctcaaa ctccgccagc acatcggtta gctgcttgtt
    18781 ggagatctgc gtgacgttcg ccattgcagc gctcagggtt tgcacttcgc gctgggagag
    18841 gtgcttgaac acctctgccg cccggtcttc gccaatggtc atcagcagga tgacgctttt
    18901 atcggtgcct gtcaggttac tcatgatcgt tatttatcca ctggcgaatg accagcgcca
    18961 ccacgcgcgg atcgttatca gacatttcac ggatacgctg gctcatgact tctgccccca
    19021 gtcgttggtt agcgcgccgt tgttggagtt gttcgtcttt gctcagacgg acttccaccg
    19081 catcttccac ttcctcgcgg gcctgcgcct gttgctgcac agtcttcacc gcctcggcgc
    19141 gacgtgttag ctgcgggcgt accgctttcc gccacagcaa ccacgccacc agcagtacca
    19201 gcaaccagcg accggcagcc agcaactgat cgataaacac ttgctgttgc cagaacggca
    19261 gtgctccgcc gctttcgtca ctgctattga acggcgagtt aacaacattg agcgaatcac
    19321 cgcgtttttc agaaaagccc atcgcctcgc gggtcagagc ttcaatttgc ttcatctgtt
    19381 cgttgctgag aggcaatggt ttaccgtctg gcaaggtttt gtaattcacc actaccgcga
    19441 ctgacagacg ttgcacatcg cccacgttca ttttggtatg acgaatggtg cgatcgactt
    19501 cgtagttact ggtttcattc cgctgtgtgc tacgcggccc actattgctg gtggtgctcg
    19561 cctgctgctg gcggttattt tgatttgccg gaggcgtgct gattggcgcg ttattcgcag
    19621 gtgccggttg attcgacaac gctcccggta cgccgcccgg ataaccggaa ccgctttgct
    19681 cgctctcatt aagctggcgt gaacgaagcg ccgcatgaga ttcatcaccg ttagggcgat
    19741 actgttcttc cgtttgttct ttactggcga agtccagttg cgccgtgacc tgggcgtgaa
    19801 tattaccgtt accaacaata ggcgacagga tcgcttcaat acgccgctga atacggcctt
    19861 cgacatcgct ggcatatttc aactgagcgt cattaagatc gcgcccgctg gtattggact
    19921 gggttaacag atgcccgccc tgatccacca gcgtgacgtt tccaggcggc agaccagcaa
    19981 ccgcgctgga aaccagatgt acaatagcgc taatttgccc ttcatcgagt gcgcggccgg
    20041 gtaacagatt taccgtcacc gatgcagaag gggatttttg ttcacggacg aataaagacg
    20101 gtttcggcat tgccagatgt acgcgcgccc cttttaccgg gccgatagtt tcgatggtac
    20161 gagaaagctc gccttccagc gcccgctgat aattcacctg ttcgctgaac tggctgatac
    20221 caaacttttc ctgatcgagc agttcgaaac cgaccgcgcc gcctttgggc aaaccttgtt
    20281 gcgccaggcg caggcgcagt tcgtgaactt tatctgccgg aacctcaata gcgccgctgg
    20341 cttcgctgaa gcggtaagga atattcattt gcgtcagttg gctgacaatt gcgccaccat
    20401 cctgatcgga aagattgctg aataatgtgc ggtagtcggg ggctttcgcc cacaggatca
    20461 gtgcgaccat gaccgccact gccgcggaac cggtaacaat caatggaatt ttcggattcg
    20521 cacgcaggcg attaagccac tcaagagatt ttgtctgggc tgcagtcgca ttcatcgcgc
    20581 acctcgtggc tgattatgga cggcgttatt ggcaaagtgg aacaagactc actcccgggt
    20641 cagcaaactc gaaaaattga cgctcccatt atttgtgctt tcgttttttt ctatccgcca
    20701 ataaacccgt ttttttgttg ctatttagcg cctttgtctt attgacttac tggtaggctt
    20761 tgttaccaga aattatccgg gagacgaaaa tgtcagcgat acaggggatt gaaggggtta
    20821 tcagccagtt acaggctacg gcgatgagtg cgcgtgcgca ggaatcactg ccgcaaccga
    20881 ccattagttt tgccgggcaa ctgcacgccg cgctcgatcg cataagcgat acacaaacag
    20941 tcgcccgcac gcaggcagaa aaattcaccc tcggtgaacc cggcgtggcg ctaaacgatg
    21001 tgatgaccga tatgcaaaaa gcctcagttt ctatgcaaat ggggattcag gtgcgtaata
    21061 agctggtggc ggcgtatcag gaagtgatgt ccatgcaggt gtaggttgtg taacctgttg
    21121 actatgcttg tttaattata tccgatccca cagcgtgggg catggatggg gcaaactcac
    21181 tcaatttctg gtttaaaatg gatacctgat cctggttgtt ttctgccatc cacgatccgt
    21241 aaacgcgata acatgttttg tcttcgcttt gctcccagga agagtgaact cattcgttag
    21301 cctggggttc cttctgatca ttattgttcc tgcctttatc tcttgccacg ccagagcccc
    21361 ctggatacac atacaccagg atgaaaacgg tgagttgtgt tcaaactgta gtacaattct
    21421 ctccagtatg aacaggaaag aatatgctat gaacccttat atttatcttg gtggcgcaat
    21481 acttgcagag gtcattggta caaccttaat gaaattttca gaggggttta cacggttatg
    21541 gccctctgtt ggcacaatta tttgctattg tgcatcattc tggttattag ctcagacgct
    21601 ggcttatata cctacaggga ttgcttatgc tatctggtca ggagtcggta tcgttctgat
    21661 tagtttgctt tcctggggat tttttggtca acgactggac ctgccagcta tcataggcat
    21721 gatgttgatt tgtgccggtg tgttagtcat taacttgttg tcacgaagtg caccgcatta
    21781 aaaaataatt atccttgaga taacaggaat ctgatttgtg catattatgg ctaatgttcg
    21841 gggcaccttt ataattatga ttgcatttgc gcgatcattg cgcctttatg cataatgaga
    21901 tcaaggcaca tcaaataaaa taacgatagg tcacaacaat gaggttttta tgaaaaaact
    21961 tatcgtttca agtgtattgg cattcataac attttctgcg caggccgcag catttcaggt
    22021 cactagtaat gaaataaaaa caggagagca acttacaacg tctcatgtat tttctggatt
    22081 tgggtgtgaa gggggtaata catcgccctc attaacctgg tctggtgctc ctgaaggcac
    22141 caaaagcttt gccgtaactg tgtatgatcc agatgctcct acaggcagtg gatggtggca
    22201 ctggactgtt gctaatattc cagcaactgt aacatatttg cccactgatg caggaagacg
    22261 tgatggtaca aaacttccga ctggtgctgt tcaaggccga aatgattttg gttatgcagg
    22321 atttggtgga gcatgtcctc ccaaaggaga taaaccccat cattaccagt ttaaagtatg
    22381 ggctctaaaa actgataaga ttcctgtgga ttctaattcc agcggagcgt tagttggtta
    22441 tatgcttaat gccaataaaa ttgcaaccgc tgagataaca ccagtttatg agataaagta
    22501 gggtaagagt atgctggcaa aagataagtc taacctaaag attgaagaaa tacggatgca
    22561 taaacatcat gagattcaca gggttaagcc tcttatgcca gctttatgtc gtatccgtca
    22621 gggaaaaaaa gttatcaatt gggagacgca tagtctaact gttgataata agcagataat
    22681 attatttccc tgtggatatg aattttatat tgcgaattat ccagaagcag gtctttatct
    22741 tgcagaaatg ctttattatc ccattgatct aattgaaaag tttcaaaaat tttatgcgat
    22801 aactgatcaa attcgtaaca cgacaggttt ctgcttacct cagaaccccg agttaatata
    22861 ttgttgggag caactaaaaa catctatttt ccgaggcttc tcaacgcaaa ttcaggaaca
    22921 cttagcaatg ggcgttctac tttcattagg agcacatcat gttaattgtt tacttttatc
    22981 agatagtaaa caatcattaa taagtcgttg ttataaccta atgctatccg aacctggaac
    23041 aaaatggaca gcaaacaagg tagcgagata tctctacatt tctgtttcca cattgcatcg
    23101 ccgtctggca agcgagggaa taagttttca aagtatattg gacgacgtga ggttaaataa
    23161 tgcgttgtct gctatacaaa cgacagtaaa acccatcagc gagattgcca gggaaaatgg
    23221 ttacaagtgt ccttctcgtt ttactgaaag gttccataat cgttttaaga taacaccaag
    23281 agagctaaga aaagcgtcca gagagtaaaa gtattttatg aaggagcaac agcatcgatt
    23341 tttattttgg aaaataaata cggcatactc aacctattgt atcctgaaca atccggtgac
    23401 ttctgcgcta atcggggacg tttataagca catataattg atactatctt cctccatcac
    23461 aatttcagaa atgcacgtgt gatttttttg catccggcgt ttggactatg tggagctcaa
    23521 gagtgggaaa gttacaagag gttgagggga taattaaagg gatacttatg acattagata
    23581 tcaggttagg tacggatatc gttgttatta aatctagttg atacgttaat tgttcactgg
    23641 gtattattaa aaatggggcg ttgaaacgcc ccagaaaata aagggtaata tataacagaa
    23701 ggtttatata gtcagaagca agatagtgct tctaaggaaa gtggcttgag ggcgccactt
    23761 atatgttggg gaggcaaagc ctcccacaac atatctttga gtaatcagat tagaactggt
    23821 aaaccaatcc tacagcaacg atatcatcgg tagcaacgcc agatgctttc gtgaaatcgc
    23881 tcttatcaat caggttgatt ttgtaatcaa caaaagtgga catatttttg ttgaagtaat
    23941 aggttgcacc tacatcaata tattcaacca ggtcctgatc accccacgca cccaagtctt
    24001 ttcctttaga ttgcaggtaa gcaacggacg gacgcagacc gaagtcgaac tgatattgtg
    24061 caactacttc gaagttttgt gctttgttgg caatatggtt attaccaaaa acagtcatgt
    24121 tctgggtttc agaataggtg gtagccagat agatgttgtt cgcatcatat ttcagaccag
    24181 ctgcccatac ttcagcattt tgaccagatg cattcaggct gttgttaccg tagataacct
    24241 gatcattagt gcggtcagat ttagcatagg ttgcacctac accgaatcct tcatactcat
    24301 aagtagtgga gaaaccgaaa ccatcaccgt tagcttcagt tacgtcagtg cggtcatttt
    24361 taccctgata ctgagcagca aagttcagac catcaaccag accaaagaag tcgttgttac
    24421 gataggttgc aacacctgtg gtgcgaccag tcatgaacac atctgtttgg gtccaggtat
    24481 cgccaccgaa ttctggcaga acgtcggtcc atgcaccgat atcgtatgct acaccgtagt
    24541 tacggccata atcgatggag ccgtagtcac cgaatttcag gccagcgaag gcaagacggg
    24601 ttttatcttt ggaagaacct tgagattcag cacggttgcc tttgaattca tattcccact
    24661 gaccgaaacc agtcagttga tcgttgattt gagtttcgcc tttgaagccc agacgaacat
    24721 aagttgtgtc gccatcatct gcatcgttag aagagaagta gtgcttagca ttaacttttc
    24781 cgtacagatc cagcttgtta ctgtctttat tgtaaatctc tgctgcctga gcagacatcg
    24841 ccatcagtac tgatgcagct acagcagaaa ttgccactgt taattttttc atcgtgagcc
    24901 cttttatttg aactattatt taaaaatgat gtcactgcac tacaaatatt catctaatca
    24961 atgagattat ttcaagatgt aagttttagt ttctcattta aattgtgaag tagatcccta
    25021 tttttatctg aactgtttct attgcacaca atgcatgact attttgtgaa cgaa
//
LOCUS       DAZKHK010000081        24864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKHK010000081 DAZKHK010000000
VERSION     DAZKHK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.081"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..2675)
                     /gene="clpB"
                     /locus_tag="ACHOS5_003211"
     CDS             complement(102..2675)
                     /gene="clpB"
                     /locus_tag="ACHOS5_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9777982.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2805..3536)
                     /gene="yfiH"
                     /locus_tag="ACHOS5_003212"
     CDS             complement(2805..3536)
                     /gene="yfiH"
                     /locus_tag="ACHOS5_003212"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP9777983.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEIAAAHAGWRGLCSGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3533..4513)
                     /gene="rluD"
                     /locus_tag="ACHOS5_003213"
     CDS             complement(3533..4513)
                     /gene="rluD"
                     /locus_tag="ACHOS5_003213"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP9777984.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            4648..5385
                     /gene="bamD"
                     /locus_tag="ACHOS5_003214"
     CDS             4648..5385
                     /gene="bamD"
                     /locus_tag="ACHOS5_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP9777985.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5655..5996
                     /gene="raiA"
                     /locus_tag="ACHOS5_003215"
     CDS             5655..5996
                     /gene="raiA"
                     /locus_tag="ACHOS5_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9777986.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6099..6224
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6100..6147
                     /gene="pheL"
                     /locus_tag="ACHOS5_003216"
     CDS             6100..6147
                     /gene="pheL"
                     /locus_tag="ACHOS5_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP9777987.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6246..7406
                     /gene="pheA"
                     /locus_tag="ACHOS5_003217"
     CDS             6246..7406
                     /gene="pheA"
                     /locus_tag="ACHOS5_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP9777988.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSAINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7449..8570)
                     /gene="tyrA"
                     /locus_tag="ACHOS5_003218"
     CDS             complement(7449..8570)
                     /gene="tyrA"
                     /locus_tag="ACHOS5_003218"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP9777989.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLALSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8581..9651)
                     /gene="aroF"
                     /locus_tag="ACHOS5_003219"
     CDS             complement(8581..9651)
                     /gene="aroF"
                     /locus_tag="ACHOS5_003219"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP9777990.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9861..10226
                     /gene="yfiL"
                     /locus_tag="ACHOS5_003220"
     CDS             9861..10226
                     /gene="yfiL"
                     /locus_tag="ACHOS5_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP9777991.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10376..10894
                     /gene="yfiR"
                     /locus_tag="ACHOS5_003221"
     CDS             10376..10894
                     /gene="yfiR"
                     /locus_tag="ACHOS5_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP9777992.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10884..12110
                     /gene="dgcN"
                     /locus_tag="ACHOS5_003222"
     CDS             10884..12110
                     /gene="dgcN"
                     /locus_tag="ACHOS5_003222"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP9777993.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNSSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLTEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12126..12608
                     /gene="yfiB"
                     /locus_tag="ACHOS5_003223"
     CDS             12126..12608
                     /gene="yfiB"
                     /locus_tag="ACHOS5_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9777994.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVISAP
                     "
     gene            complement(12685..13032)
                     /gene="rplS"
                     /locus_tag="ACHOS5_003224"
     CDS             complement(12685..13032)
                     /gene="rplS"
                     /locus_tag="ACHOS5_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9777995.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13074..13841)
                     /gene="trmD"
                     /locus_tag="ACHOS5_003225"
     CDS             complement(13074..13841)
                     /gene="trmD"
                     /locus_tag="ACHOS5_003225"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9777996.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13872..14420)
                     /gene="rimM"
                     /locus_tag="ACHOS5_003226"
     CDS             complement(13872..14420)
                     /gene="rimM"
                     /locus_tag="ACHOS5_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9777997.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14439..14687)
                     /gene="rpsP"
                     /locus_tag="ACHOS5_003227"
     CDS             complement(14439..14687)
                     /gene="rpsP"
                     /locus_tag="ACHOS5_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9777998.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14824..16185)
                     /gene="ffh"
                     /locus_tag="ACHOS5_003228"
     CDS             complement(14824..16185)
                     /gene="ffh"
                     /locus_tag="ACHOS5_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9777999.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16351..17142
                     /gene="ypjD"
                     /locus_tag="ACHOS5_003229"
     CDS             16351..17142
                     /gene="ypjD"
                     /locus_tag="ACHOS5_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP9778000.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17163..18449
                     /gene="yfjD"
                     /locus_tag="ACHOS5_003230"
     CDS             17163..18449
                     /gene="yfjD"
                     /locus_tag="ACHOS5_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP9778001.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18504..19097)
                     /gene="grpE"
                     /locus_tag="ACHOS5_003231"
     CDS             complement(18504..19097)
                     /gene="grpE"
                     /locus_tag="ACHOS5_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9778002.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNILGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19220..20098
                     /gene="nadK"
                     /locus_tag="ACHOS5_003232"
     CDS             19220..20098
                     /gene="nadK"
                     /locus_tag="ACHOS5_003232"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP9778003.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20184..21845
                     /gene="recN"
                     /locus_tag="ACHOS5_003233"
     CDS             20184..21845
                     /gene="recN"
                     /locus_tag="ACHOS5_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9778004.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQLHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            21994..22335
                     /gene="bamE"
                     /locus_tag="ACHOS5_003234"
     CDS             21994..22335
                     /gene="bamE"
                     /locus_tag="ACHOS5_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9778005.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22397..22687)
                     /gene="yfjF"
                     /locus_tag="ACHOS5_003235"
     CDS             complement(22397..22687)
                     /gene="yfjF"
                     /locus_tag="ACHOS5_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP9778006.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22677..23114)
                     /gene="ratA"
                     /locus_tag="ACHOS5_003236"
     CDS             complement(22677..23114)
                     /gene="ratA"
                     /locus_tag="ACHOS5_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP9778007.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23285..23767
                     /gene="smpB"
                     /locus_tag="ACHOS5_003237"
     CDS             23285..23767
                     /gene="smpB"
                     /locus_tag="ACHOS5_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9778008.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVSDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            23982..24344
                     /gene="ssrA"
                     /locus_tag="ACHOS5_003238"
     tmRNA           23982..24344
                     /gene="ssrA"
                     /locus_tag="ACHOS5_003238"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24607..24855
                     /locus_tag="ACHOS5_003239"
     CDS             24607..24855
                     /locus_tag="ACHOS5_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9778009.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
BASE COUNT         6373 a         6497 c         5823 g         6171 t
ORIGIN      
        1 aaaactttat tgttcgctca tttttcaggc aaaacatctt taatatagtc ttttccgtct
       61 aacttataga caaaaacgag ccccgaaggc tcgttttatc attactggac ggcgacgatc
      121 cggtcttcat taacttccag gcgaataact ttacccggaa ccaattcacc agacagtatt
      181 tgctgtgcca gcgggttttc gatctgctgc tggatagcac gcttcagagg acgtgcacca
      241 tagaccggat cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt
      301 tcataaccac gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgac
      361 gcaatgtgtt gttcacccag cggatggaag acgacgactt catcgatacg gttaatgaat
      421 tccggacgga agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc
      481 agttcaccaa aacgttcctg aatcagatca gaaccgaggt tagaggtcat aatgacgacc
      541 gtattacgga agtcgaccgt tctcccctgc ccgtcagtca gacgcccatc atccagtacc
      601 tgcaacagaa tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg
      661 gaatacggac gacgacgcac cgcttcggtc agatagccac cttcttcata accaacatat
      721 cccggaggcg caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatca
      781 atacggacca tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctct
      841 gttttcccca cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc
      901 gccagccccg cacggctacg acgaatagcg ttagataccg catcaaccgc ttcgttctga
      961 ccaataacgc gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc
     1021 atcatgcgag aaaccggaat tcccgtccaa cgcgccagca cttcagcaat ttcggcatcg
     1081 gtcactttat tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt
     1141 tgcttttcca gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtcaccc
     1201 acacggcgag cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc
     1261 gtaccagaaa gcgaggcttt ctctgctttc cactcttctt ctaactcgga gtactgacgt
     1321 tctttgtcgc tcagttcttc gttgagcata tccaagcgtt ttttactggc ttcatcagac
     1381 tctttcatta acgcctgttg ttccagtttg agctggatga tacgacgatc aagtcggtcg
     1441 agttcttctg gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg
     1501 tcgatggctt tatccggcag ttgacggtca gcaatgtagc gatgagacaa cgtcgctgct
     1561 gcaacaattg ccgggtcagt aatttgcaca tggtggtgca gctcgtaacg ttctttcagg
     1621 ccacgcagaa tagcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa
     1681 cgacgttcca gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca
     1741 cctacgcagt gcaattcacc acgcgccagt gcaggtttca gcatgtttcc ggcgtccatt
     1801 gcgccatcgg ctttaccagc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg
     1861 ttgccttcct gtttagcaag atcgttaagt acgcctttta aacgttcttc aaactcaccg
     1921 cgatatttcg ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc
     1981 aacccttcgg gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt
     2041 ttaccgacgc cgggttcacc aatcaatacc gggttatttt tagtacgacg ttgcagcacc
     2101 tgaatggtac ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccttgttcg
     2161 gctcgttcgg taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct
     2221 tgatcgttca cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg
     2281 gtcgcccctg ctgctttcag gatgtcagcc agcgtgccgc gagactcaag tgccgccaga
     2341 acgaacagtt ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga
     2401 ttaagaacgc gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc
     2461 ggtaaacgat ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg
     2521 gatgttaata aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga
     2581 agtggttcga tgaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca
     2641 agctggaatt tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt
     2701 tgctactgga gattaaatga ggtcatccct caattattca aggttattga ccagattaat
     2761 gtgaaaagaa aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg
     2821 aaacttgcca tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc
     2881 gtatatgtac aacggtcgcc gccgaaaatt tgttcaacac caacgctcgc cagacgctgc
     2941 cgggcaagct gataaatatc tgccagatac ttatcaccat gctgaatgaa agctgtactc
     3001 gctttggcgt ctactgccat aaacgcctcg cgaacctccg cccccacttc aaacgcgcgt
     3061 ggaccaattg ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa
     3121 accgtctctt ccagcacgcc tgagcacagt ccacgccagc cagcatgagc ggcggcgatt
     3181 tccgttcccg ctcgattgca aaacagcacg gggaggcagt cggcagtcat cactgcgcaa
     3241 accgtgcccg gcgtgttgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca
     3301 gtaagcttaa gcacatcttt gccgtgtacc tgctcaagcc agaccggttt tgaaggcaaa
     3361 ttgcccgcag caaaaagtcg cttgcgattc tcttcaacgt gatccgggtt atcgccacaa
     3421 tgagcaccga ggttgagcga atcatacggg ggcaagctca cgccgccgat acgagtggag
     3481 ctacaggccg caacaccttt tggcagcggc cactgcggga caatcagctt actcataacc
     3541 agtccacttc atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca
     3601 tatcttctgg aataggcgca tgccattcca tttcgatacc ggagatcggg tgataaagac
     3661 gcagcatggt tgcatgaagc gcctggcggt caaacttacg cagcgtggag ataaacgctt
     3721 ccgaagcacc ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat
     3781 gagtgatatg ggccatatgc acgcggatct ggtgcgtacg tccggtttcc agacgcaacc
     3841 gcagacgcgt gtgcacacgg aagtgttcca tgatgcgata gtgagtgacc gccggtttac
     3901 ccatcggatg caccgccatg tgagtacgtt tggtcgggtg gcggctgatt ggttcatcta
     3961 ctgtaccgcc agcggtcata tgaccaattg ccaccgcttc atactcacga gtaatttcac
     4021 gccgttgcaa agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca
     4081 ggccggtggt gtctttatcc agacgatgga cgatgcccgc acgcggtaca tcggcaatgg
     4141 gcggataata atgaagcaac gcattcagta ccgtaccatc cgggttaccc gcgccaggat
     4201 gtaccaccag gtcgcgtggt ttattaatga cgatgatatc ttcatcttca tagacgatat
     4261 ccagcgggat atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcca
     4321 cctgctcgcc acccaatact ttttctttcg gcttatcaca aactttgccg ttaaccagca
     4381 cgcgctggtc gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg
     4441 ccaaagcctg atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta
     4501 ctcgttgtgc catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt
     4561 taatatagtg tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta
     4621 ttacattttg aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc
     4681 acactaagcc tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg
     4741 ccaaatgaaa tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca
     4801 ataacgcaac tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg
     4861 cagctggatc tcatctacgc ctactataaa aacgccgatt tgccgttagc acaggctgcc
     4921 atcgatcgtt ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg
     4981 cgtggcctga ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac
     5041 cgtagcgatc gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg
     5101 cgcggctatc cgaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa
     5161 gatcgtctgg cgaaatatga atactccgtt gccgagtatt atacagaacg tggtgcatgg
     5221 gttgctgtcg ttaaccgcgt agaaggcatg ttgcgcgact atccggatac ccaggctacg
     5281 cgtgatgcgc tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct
     5341 gaaaaagtag cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaaca
     5401 caaaacggca gccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt
     5461 tagtcactat cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac
     5521 acctcttcct gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag
     5581 atttccatca cacattttga catcaggaac gttatgctga attcaccaag acgggaagac
     5641 aagaggtaaa atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca
     5701 tccgccaaca tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta
     5761 atccacatat cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata
     5821 cacctaacgg cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg
     5881 aattgatcaa caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc
     5941 gtgccgcaac atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct
     6001 ttatattgag tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac
     6061 agtacgggta ctgtactaaa gtcacttaag gaaacaaata tgaaacacac accgtttttc
     6121 ttcgctttct tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc
     6181 gaagacgaac aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca
     6241 acactatgac atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg
     6301 aaaaattatt agcattactg gcagagcgac gcgaactggc cgtcgaggtg ggaaaagcca
     6361 aactgctctc gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat
     6421 taattacgct cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc
     6481 tcatcattga agattccgta ttaactcagc aggctttgct ccagcaacat ctcaataaaa
     6541 ttaatccgca ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg
     6601 cggcgcgcca gtatgctgct cgtcactttg agcaattcat tgaaagtggc tgcgccaaat
     6661 ttgccgatat ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg
     6721 aaaataccag ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagtttgt
     6781 cgattgttgg cgagatgacg ttaactatcg atcattgttt gttggtctcc ggcactactg
     6841 atttatccgc catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat
     6901 tccttaatcg ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg
     6961 aaaaggttgc acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca
     7021 ctttgtacgg tttgcaggta ctggagcgta ttgaagcgaa tcagcgacaa aacttcaccc
     7081 gatttgtggt gttggcacgt aaagccatta acgtttctga ccaggttccg gcgaaaacga
     7141 cgttgttaat ggcgacggga caacaagctg gtgcactggt tgaagcgttg ctggtactgc
     7201 gcaaccacag tctaattatg acccgtctgg aatcacgtcc gattcacggt aatccgtggg
     7261 aagagatgtt ttatctggat attcaggcca atcttgaatc agcggaaatg caaaaagcat
     7321 tgaaagagtt aggggaaatc acccgttcga tgaaggtatt gggctgttac ccaagtgaga
     7381 acgtagtgcc tgttgatcca acctgatgaa aaagtgccgg atgatgtgaa tcatccggca
     7441 ctggattatt actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga
     7501 aaacgctgtg cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc
     7561 tgcttatcgc cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg
     7621 attaacgcca gattacgctc tgacgacata ataatgtcgg cataaagctg cggatcctga
     7681 gcaaacagtc gcccaaccat cgccagttca aggcggtaaa tcggcgaaga gagcgccaga
     7741 agttgctcaa gctgaacatt ttcttctgcc aggtgcagcc cataagcaaa agtagcaaag
     7801 tggcgcagag cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc
     7861 agccgagcgc cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt
     7921 ccatcacacc agaccacaac ttgctttgcc aggctaccgc tgtccggacc gaacatcggg
     7981 tgtagcccca gcaccggacc atcatgcgcc gccagcatgg cctgtaatgg cccatttttc
     8041 actgatgcca gatcgaccag aatacaatct ttcggtaaag gcggtaattt gccgataact
     8101 tgctcagtaa cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca
     8161 gccgctcgat cccagtcatg ttgctccaga atccgcacct gataacccga gagtgccagc
     8221 atcttctcga acagacgtcc catctgaccg ccaccaccga cgataaccac cggacgcagt
     8281 gacggacaaa gcgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc
     8341 cgacgcaaaa catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacgc
     8401 cgcgaggcca gcatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt
     8461 ttcacctcgc caacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct
     8521 ttatcgactt catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc
     8581 ttaagccacg cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc
     8641 atcggtcatt tcccaactaa tgcaggcatc ggttacggat acaccgtatt tcatttcact
     8701 gcgcggttgc tcggaagact gattgccctc gtggatatta ctttcgatca tcagaccaat
     8761 aattgagcga ttgccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg
     8821 gcgataatct ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc
     8881 cgcctgttcc atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt
     8941 accaccgcgc aggatcacat gcccgtccgg attcccctga gtttgtagca acgccacctg
     9001 ccctgcctgg ttaatgccaa caaaacggtg cggctgggcg gcggcgcgca tagcgttaat
     9061 tgctgttgcc agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga
     9121 ggccatttca cggtgtgttt gcgattccgt tgtacgagca ccaattgctg accagctaaa
     9181 cagatcgccc aggtattgcg ggctattagg atctaacgct tccgtcgcca gtggcagtcc
     9241 catattcacc agctcaagca gcaatttacg cgcgatctgc agcccggctt ctacatcaaa
     9301 agagccatcc atatggggat cgttaatcaa ccctttccaa ccgacagtgg tacggggttt
     9361 ttcaaaatag acgcgcatta ccagatagag gctatcgctg acctctgcgg caagggcttt
     9421 aaatcgacga gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac
     9481 taccaacaga cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc
     9541 aatctgggct tcctgttgca ggcttaatgg aaaagcggcc ttcagttgtt ccggagtcat
     9601 taaaacttgt tcgtcggtaa tatgtacgtt attcagcgcg tctttttgca tgatggcgat
     9661 cctgtttatg ctcgtttgcg atagttgatc ctcagcgagg atgacgtaac gataacacat
     9721 aaagtaaagt tttcaatcca tatttcgtac atttttcttt acaaaagcaa tttattcgcg
     9781 cttttaaccc ttgtttatgt ataaataaat ttacaccacc tttgaaaaca atccgctatg
     9841 ctttgaaaaa ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg
     9901 ttagcggatg tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt
     9961 atgatgttgg tatggaagat gcgatatcgg gcaacgccat aaaagatgac gatgcattta
    10021 gcgattcaca ggcggatcgc ggtctatacc ttaaaggata tgccgaagga caaaagaaaa
    10081 cttgccagac cgattttact tatgcccgag gactttccgg taaaagcttt cctgccagct
    10141 gcaacaatgt tgaaaatgcc agccaactgc atgaagtatg gcaaaaaggg gctgatgaaa
    10201 atgccagcac aatacgatta aattaattaa acccccacaa ataaattaaa catgagattc
    10261 tactcattgt gttttatttc tcacattgat gacgaccgca tgaaagaatg atattttaat
    10321 gagcattctg aaaaaatatc gatgcatttc gagcgaagat ggtgaggatc cctgaatgcg
    10381 tttttctcac cgactgttct tactgctgat tctcctgctg acaggagcac caatactcgc
    10441 tcaggaacct tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta
    10501 cacccgctgg cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacattt
    10561 tagtaacgcc ctacaagaga atggtacgac atctttgcct tatctacctg tcatcattca
    10621 cactcaacaa gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc
    10681 gacattccag atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga
    10741 acaaaatacc gagtgcatta ttggtagcgc cttttgtcta atcatccaca ataatgacgt
    10801 cagatttgcc gtaaacctgg atgccttatc gcgtagcggt gtaaaggtca acccggatgt
    10861 cttaatgctc gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca
    10921 cgtttaaaag agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga
    10981 tctggttgct gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc
    11041 tggcactgac agcagcaaca atgacttaca gtctggaagc agcggtcgtt tttgccgatg
    11101 gccctgcagc aactgaaaca ctggcagcgc tggggcagca aggccaattt tcaactgcag
    11161 aagtgcgtga taagcagcaa aatattctgg catcctggca ttacacccgt aaggatccag
    11221 gcgatacttt cagtaatttc ataagccact ggctcttccc tgcccccatc attcagccga
    11281 ttcgtcacaa tggtgaaacc attggcgaag ttcgcttaac cgctcgcgac agttcaatca
    11341 gccatttcat ctggttttcg ctcgctgtcc tgaccgcctg cattctgcta gcgtcaggca
    11401 tcgcaattac cctcacccgc catttgcaca atggtctggt tgaagcgtta aaaaacatca
    11461 ccgatgtcgt acatgatgtg cgttccaacc gcaatttttc ccgacgagtt tcggaagaac
    11521 gtatcgcaga gtttcaccgc ttcgctctcg acttcaacag tctgctggat gaaatggaag
    11581 agtggcagct tcgtttacag gccaaaaatg cgcagcttct acgtaccgca ctacatgacc
    11641 cattaaccgg gcttgctaac cgcgcagcgt ttcgtagcgg tatcaacacg ttgatgaaca
    11701 gttccgatgc ccgaaaaacg tcggcgttac tatttcttga tggcgataat ttcaaatata
    11761 tcaatgatac ctggggtcat gcgacgggcg atagagtctt gattgaaata gcaaaacggt
    11821 taactgaatt tggcgggctg cgacataaag cataccgcct gggcggcgat gaattcgcta
    11881 tggtgctcta tgatgtacaa tcggaatctg aagtgcagca gatatgctca gcactgacac
    11941 aaatttttaa tctcccgttt gatcttcata atggccatca gactaccatg acattaagca
    12001 ttggttacgc gatgaccatt gagcacgcct ctgcggaaaa tttacaagag cttgccgatc
    12061 acaatatgta tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa caaggatata
    12121 tcgatatgat aaagcacctg gtagcacccc tgattttcac ctcgctaata cttactggct
    12181 gccagtcccc tcagggaaag tttactcctg agcaagtcgc cgctatgcaa tcttatggat
    12241 ttactgaatc cgccggcgac tggtcgctgg gcttatcaga tgccattctg ttcgcaaaaa
    12301 atgactacaa attgctcccg gaaagccagc aacaaatcca aaccatggca gccaaattgg
    12361 cctcgacagg gctaacacat gcccgtatgg atggacacac cgacaactat ggtgaagaca
    12421 gttacaacga agtcttatca ttaaaacggg cgaatgtcgt ggccgatgca tgggctatgg
    12481 gtggacaaat tccgcgcagc aatctcacca cacagggttt aggaaaaaaa tatcccatag
    12541 ccagtaacaa gaccgcccag ggccgcgccg agaaccgccg cgtcgctgtg gtgattagtg
    12601 caccgtaaac gcaattttga tagataaaaa aagggccagc caattggcca gcccttctta
    12661 acaggatgtc gcttaagcga aatcttagtt aagacgctct ttgatacgag cagccttacc
    12721 agtacgctca cgcaggtagt acagtttagc tttacgaaca gcaccacgac gtttgacaga
    12781 aatgctgtca actaccggag agtgagtctg gaagacacgc tcaacgcctt cgccgttgga
    12841 aattttacga acagtgaatg cagagtgcag accgcggtta cgaatagcga taaccacgcc
    12901 ctcgaatgcc tgcagacgtt ttttggaacc ttcaacaacc catactttca cttccacggt
    12961 atcacccgga cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa gttgcttaat
    13021 aatgttgctc ataatttaat ctcttatcct gggtaaactg atatctcggg ggcttacgcc
    13081 atcccatcat gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc cagcaacctt
    13141 gcttgctctt cagtcagagc caggttttcc agaagttcag gtcttctaag ccaggtacgg
    13201 cccagcgact gtttcaaacg ccagcgacgt atctcggcat ggttgcccga cagtaacact
    13261 ggcggaactt ccatcccttc taacacctca ggccgcgtat agtgcgggca atccagcaat
    13321 ccttcagcaa aggaatcttc cgttgccgag gcctcatgtc ccagtactcc cggaataaac
    13381 cgggaaacgg agtcaatcag cgtcattgct ggtaactcac caccactgag aacgtaatcg
    13441 ccgattgacc attcttcatc aatttcggtt tggatcacgc gctcatctat accttcgtag
    13501 cgaccgcaca ccagaatcaa tttttgattc gttgccagct cgctgacgcc cgcttgatca
    13561 agcttgcgtc cctgtggtga cagataaatc acctttgcgc cttcacccgc cgcggctttt
    13621 gctgcatgaa tggcgtcccg caagggttgc accatcatta acatccccgg tccgccgccg
    13681 taaggacgat cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg actccagctc
    13741 tggatgctca gcaggccatt tttaactgcc cggccagtta ccccgtaatc ggtaattgcg
    13801 cggaacattt caggaaacag gctaattatg ccaatccaca tagcgccgtc ttttaccgtt
    13861 tatccggtgg tttaaaaacc aggatcccaa tctacttcga ttgaacgagt agtgagatcg
    13921 actttcttga taacctgccc atcgaggaac ggtacgagac gttccttgat accaaacgca
    13981 tctttcaggt ttgccttaat gacgagaacg tcattagatc cggtttccat catatcgacg
    14041 actttaccga gatcgtagcc ttcagtggtt actacctggc agcccatcag gtctttccag
    14101 tagtagtcac cctcttcaag ctgaggcaac tgcgatgaat ccacgacaat ttcacaattc
    14161 gtcagcaggt tcgccgcatc acgatcgtca acgcctttca gcttgatgat catgtcctga
    14221 ttgtggtgct tccagctttc cagctggact tgctgccact gacccgcctt ctggataaac
    14281 cagggctgat agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac tctgagccac
    14341 ccacgaatac cgtaagacga acccattttt cccaaaacga tgggatcaac aggtgcttgc
    14401 gcggtgagtt gtttgctcat catgaccacc gtgacagatt aagctgcttt gtttacttct
    14461 ttgatcagcg cagcaacgcg atcagaaata gttgcgccct ggccaaccca gtgagcgatg
    14521 cgatccagat ccaggcgagt gccttcttct ttttcgctag cgattgggtt gaagaaacca
    14581 acgcgctcga tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac aacctggtag
    14641 aacggacgct ttttagcgcc gtgacgtgct aaacgaatag ttaccataac atcctcttgt
    14701 gtgaataaaa caaccggacc ccatcgagga acggagtccg gtgtcatatt aaaagcccga
    14761 aaattttact gatttctgga gaaaatgcaa tcagcagtta ataactctgt cgcataaggt
    14821 catttagcga ccaggaaatc ccggcggcat catccctttc atgcttctca tcatctttgc
    14881 cattccgccc ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact gtttcagaag
    14941 acggttaacg tcctgcacct gcataccgca accggcagcg atacggcgtt tacgcgaacc
    15001 tttgatgatt tctggcttag cgcgctcttt catcgtcatc gagttgatga tggcttccat
    15061 acgcaccagc actttatcgt ccatctgcga cttgacgtta tccgggatct gccccatgcc
    15121 cggcagtttg cccatcaggc tggccatgcc gcccatattt ttcatctggc gcagttgctc
    15181 aaggaagtca ttgagatcga agccgtcgcc ttttttcagc ttgctggcta atttctcagc
    15241 ttgcgcgcgg tcaactttgc tttcgatatc ttcgatcagc gacagtacgt cgcccatgcc
    15301 gagaatacgg gaagcgatgc ggtccggatg gaacggctcc agcgcctcgg ttttctcgcc
    15361 aacaccgagg aacttgatcg gtttgcccgt aatgtgacga atagagagcg ccgcaccgcc
    15421 acgagcatcg ccgtcgactt tagtcagtac cacaccggtc agcggtaacg cttcattgaa
    15481 tgcttttgcc gtattggccg catcctgacc agtcatggca tcaaccacaa acagggtttc
    15541 aaccggatta atcgacgcat ggacttgttt gatctcgtcc atcatcgctt cgtcaacgtg
    15601 cagacgaccg gctgtatcca ccagcagcac gtcgtagaat ttcagtttgg cttctttcag
    15661 cgccgcgtta acgatatcta ccggcttctg accaacatca gaagggaaga aatcaacgcc
    15721 cacctgctct gccagcgtct caagctgttt gattgccgcc ggacgataaa cgtcggcaga
    15781 aaccaccagc actttcttct tgtgcttctc gcgcaggaac ttaccgagtt taccaacgct
    15841 ggttgtttta ccggcacctt gcaggcccgc catcagcacg accgcaggcg gttgcgcagc
    15901 caggttcagg gtctggttct cttcgcccat cgccgcaacc agttcgttac ggacaatttt
    15961 gacgaactcc tgccccggcg tcaggctctt attaacttca tgaccaaccg ctttctcttt
    16021 tacgcgattg ataaactcac gcactaccgg cagagcgacg tccgcctcca gcagcgccat
    16081 gcgcacttcg cgcagcgtat cttttacgtt gtcttcagtg aggcgtccac ggccactgat
    16141 attgcgcagc gtgcgcgaca aacgatcggt taaattatca aacattgtct ctcgcctggg
    16201 gtggaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa
    16261 ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc
    16321 tttcttattg ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc
    16381 gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg
    16441 cggcgcatgg ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa
    16501 gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca
    16561 ttggtcagtt tgatgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg
    16621 ttgctgctac ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg
    16681 cccaatgaat acatcaccca tctggaagca acgcctggga tgctggtgca cattggctta
    16741 tcgctctttt cctatgccac gctgattatc gccgccctgt acgcgctgca actggcgtgg
    16801 attgattacc aactgaagaa caagaagctg gcgtttaacc aggaaatgcc gccattgatg
    16861 agtatcgagc gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg
    16921 ctttgcactg gcctgttcta catgcacaac ttatttagca tggaaaatat cgacaaggcc
    16981 gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttatcatgaa
    17041 ggatggcgtg gacgccgcgt cgtctggttt aacgttgcgg gtgcagtcat tctgacactg
    17101 gcctacttcg gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc
    17161 ccctggaaca catttctact actacgttga tcattattct gatcatcatg gtggtcattt
    17221 cagcctattt ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtctgcgac
    17281 atatggcgaa acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag
    17341 accgcctgat tagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc
    17401 taggcactat tgttgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg
    17461 tgctgacttt tgtcgtgctg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt
    17521 acccggaaaa agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga
    17581 tgccgctggt ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca
    17641 aaaccgatat cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcacg
    17701 aatcgcgctc acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg
    17761 aaaaaatgac cgttgatgac atcatggtgc cgcgcagtga aattatcggt attgatatca
    17821 acgatgactg gaaatcgatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc
    17881 tctaccgtga ttcgctggac gacgccatca gtatgctgcg tgtgcgtgaa gcctggcgac
    17941 tcatgtcgga gaaaaaagag ttcaccaaag aaaccatgct acgcgccgcg gacgagatct
    18001 attttgtccc ggaaggtacg ccgctcagca cgcagttggt gaagtttcag cgcaacaaaa
    18061 agaaagtcgg cctggtcgtc aacgagtatg gagacattca gggactggtg acggtcgaag
    18121 atattctcga agagattgtc ggcgatttca ccacgtcgat gtcgccaaca cttgccgaag
    18181 aggtcacgcc gcaaaacgac gggtcggtga ttatcgatgg caccgccaac gtgcgagaaa
    18241 ttaacaaagc ctttaactgg catctaccgg aagatgatgc tcgtactgtt aacggcgtca
    18301 ttctggaagc gctggaggag atcccggtag caggcacccg cgtgcgtata ggcgagtacg
    18361 atatcgatat tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtaa
    18421 aaccgctgcg cgagagtgtg gcggagtaac gaaaacggcc cggcattcgc atgctgggcc
    18481 gtgatttatt acgaaagcag aaattaagct tttgctttcg ctacagtaac catcgccgca
    18541 cgaatcgtac gaccgttcag cgtataacct ttctgcataa tgcccagtat gttacctggc
    18601 gcaacgtcat cagattccac cattgcgatg gcctgatgca catttgggtc cagtgggacg
    18661 ttggtttcgg cgatcacttc aacgccaaac ttacgcacaa catccagcat cgacttcagc
    18721 gtcagctcaa taccttcaac catcgcggac atatccgggt tagctttatc agccacttcc
    18781 agcgcacgat ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg
    18841 aatttgtggg ctttttcgat atccagttca gtacgacgac gcaggttttc catttcggct
    18901 tttacgcgca aaatgccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga
    18961 ttcgcaattt tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc
    19021 tcaatctctt cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc
    19081 tgttctttac tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc
    19141 ttattatggg gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg
    19201 caacaaggac ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc
    19261 acggcacccc actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg
    19321 ttacgaggtc atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac
    19381 tggcacgctc gcggagattg ggcaacaggc tgatctcgcg gttgtcgttg gcggcgacgg
    19441 taatatgctg ggcgcggcgc gcacgctcgc ccgctacgat attaaagtta ttggaatcaa
    19501 ccgtggcaac ctgggtttcc tgactgacct tgatcccgat aacgcccagc aacaattagc
    19561 cgatgtgctg gaaggccact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg
    19621 tcagcaagat tgccagaaac gcatcagcac ggcgattaac gaagtggtac ttcatcctgg
    19681 caaagtggcg catatgattg agttcgaagt gtatatcgac gagatctttg cgttttcgca
    19741 gcgttccgat ggactgatta tttcgacgcc aacaggctcc accgcatatt ccctctctgc
    19801 gggcggtcct attctgacac cctctctgga tgcgattacc ctggtgccca tgttcccgca
    19861 tacgttgtca gcacgaccac tggtcataaa cagcagcagc acgatccgtc tacgtttttc
    19921 gcatcgccgt aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga
    19981 aggtgaagat gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga
    20041 ttacagttat ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt
    20101 ttacgccagc ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg
    20161 gtttttcata caggaaaaca actatgttgg cacaactgac catcagcaac tttgctatcg
    20221 ttcgtgagct tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggcg
    20281 cgggtaaatc tattgcaata gatgccctcg gcctttgtct cggtggtcgc gctgaagccg
    20341 acatggtgcg taccggcgct gctcgtgccg acctgtgcgc ccgtttttct ctgaaagata
    20401 cgccagcggc cctgcgttgg ctggaagaaa accagcttga agacgggcat gaatgtttgc
    20461 ttcgtcgcgt aatcagcagc gatggtcgct cccgtggttt catcaacggt acagctgttc
    20521 ctctgtcaca actgcgcgaa ctgggtcagt tgctgattca gctccatggt cagcacgctc
    20581 atcagttact caccaaaccc gagcaccaaa aattcctgct tgatggctat gccaatgaaa
    20641 cctctcagct ccaggaaatg accgcacgtt atcatttgtg gcatcaaagc tgccgtgacc
    20701 tcgcgcatca tcaacagtta agtcaggaac gcgccgctcg tgcggagctg ctgcaatacc
    20761 aattaaaaga acttaacgaa tttaatccgc agcctggaga gtttgagcaa atcgacgaag
    20821 agtacaaacg tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat
    20881 taatggctga cggtgaagac gcaaacctgc aaagtcaact ttacacggct aaacaactgg
    20941 tgagcgaatt gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagagg
    21001 ctaccatcca gattgctgaa gccagtgatg aactgcgcca ctactgcgat cgtctggatc
    21061 tcgatcccaa ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac
    21121 gtaaacatca cgtcagccct gaggcattgc cacagtatta ccagtcacta ctggaagaac
    21181 agcagcaact ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac
    21241 atcatcagca ggcactggaa acggcgcgcg cgttacacca gcaacgccag cattatgcaa
    21301 atgagttggc acagctgatc accgatagta tgcatgcact ctctatgcca catgggcagt
    21361 ttacgatcga tgttaaattt gacgagcatc acctgggcgc tgacggcgct gatcgtattg
    21421 agtttcgggt aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg
    21481 gtggtgagtt gtcccgcatc gcattggcaa tccaggtcat taccgcgcgt aaaatggaaa
    21541 ccccggcact gatttttgat gaagtggatg tagggattag cggcccaaca gcggcagttg
    21601 tcggcaaact gctacgtcaa cttggcgaat caactcaggt gatgtgtgtt acccacctgc
    21661 cacaagtcgc gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga
    21721 tgacagaaac gcatatgcaa tccctggata aaaaagcgcg gttacaagag ttggcgcgcc
    21781 tgcttggtgg cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcgg
    21841 cgtaaacttt tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa
    21901 gcaaaaggtt ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg
    21961 tactgctcgg gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca
    22021 gcagcagtac tattgatgtt gaccgcaggc tgttccactc tggagcgagt ggtttaccgt
    22081 cctgacatca accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc
    22141 atgacgcaac aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt
    22201 acaaatacct ggttctatgt cttccgccag caacctggtc atgaaggtgt aacgcagcaa
    22261 acgctgacgc tgacctttaa cagtagcggt gtgttgacca acattgataa caaacctgcg
    22321 ctgagtggta actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc
    22381 accttttttc tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt
    22441 tcggatcggc aatgagagga cgataaatct ccacccgatc gccatcatgc acaatatcgc
    22501 tgagttttgc cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac
    22561 gcaattccag caaaccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcaacg
    22621 tcacgcgctg caggtactgc ttctcaggta gcgcataagc cacctcaacg gcaattttac
    22681 ctggcactgt agacctcttt tgcacgaaca gtaaaagcct gcaccatatt agctgccagc
    22741 tctttaaaca cacgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga
    22801 tgaaactcaa tacggcacgc gtcctggctc aacggcgtaa acttccaacc gccgatcaat
    22861 ttcttaaacg gtccatcaac cagactcatg agaatacttt gattactggt caactggttg
    22921 cgggttgtaa acgttttgct gatcccagcc ttagacacat ctaccgcagc agtcatctgc
    22981 ccaggagtgg actccagaat ccgacttccg gtacaacccg gcaaaaactg aggataagac
    23041 tgaacgtcat tcactaactg atacatttgc tccgcgctgt agggcaccag tgcggtccga
    23101 ctaatctgag gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct
    23161 gctgtaaaaa aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc
    23221 ttgtcccccg caggattgat atggggtgtt ttcgatttca gattaccgat gattcacgac
    23281 gcttatgacg aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg
    23341 cgcccgtcac gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg
    23401 ggaggttaaa tccctgcgcg caggaaaagc caatatcagc gacagctatg tccttctgcg
    23461 cgacggagag gcatttctgt ttggcgctaa catcacgcca atggctgtgg cctccacgca
    23521 tgtggtgtcc gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc
    23581 attgtacggt cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa
    23641 aaatgcctgg tgcaaagtga aaatcggcgt cgccaaaggt aagaaacagc acgataaacg
    23701 ttcagatatc aaagaacgcg aatggcaggt ggataaagcg cgtatcatga aaaatgccca
    23761 ccgttaaacc tatataagcg atactaatca aatcctcacc gcgcctccct atccggtggc
    23821 gcgaatgaac atcttatcga ctgtcaaatc tgacacagat gtagccatcc cattgcttaa
    23881 tcgaaaaaaa atcatactat gcaggcgcta attccaggat gataacgcct tttgggctag
    23941 tcatgctgct cataaatctg gtatacttac cgttacacat tggggctgat tctggattcg
    24001 acgggatttg cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca
    24061 aaaaatagtc gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc
    24121 tctctcccta gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc
    24181 gcgtggaagc cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg
    24241 tgtgtccgtc cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga
    24301 tgtaggaatt tcggacgcgg gttcaactcc cgccagctcc accaaatata acaggattac
    24361 agctagactt taacagtaaa aacaagaaat tacagacatt gaatagactg taggtagacc
    24421 tgaaaaagac aaaaatatac acgcaatatg cacgtgcatt ttaaaaaacc ccagatttct
    24481 ccgtctgggt tttttctatt tgtaaaaaag tgaaacagat tacattacaa taatgacgct
    24541 cttgacactg tttatgcata cagtaaaaaa ttattcctgt acataaccac agtgacagga
    24601 ggtgttatgc gtattgagat ctgtatcgct aaagaaaaaa tctgcaaaat gccaaaaggc
    24661 tctgttcctg ccctgcaaca ggaaatgcta cgacgtgtcg gtaagcgtta tgatgatgta
    24721 gatgtgattg tgaaatctgc cagtaatgat ggactcacaa ttttaagagc cgtggataaa
    24781 gaatccgcgc aagaatttgt acaggaaacg ctgcaggaag tctgggaaac agcggatgac
    24841 tggtttatcc ggtgacatca gcac
//
LOCUS       DAZKHK010000082        24192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKHK010000082 DAZKHK010000000
VERSION     DAZKHK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.082"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            174..2417
                     /gene="pdeF"
                     /locus_tag="ACHOS5_003240"
     CDS             174..2417
                     /gene="pdeF"
                     /locus_tag="ACHOS5_003240"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP9778010.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2456..3997)
                     /gene="ppx"
                     /locus_tag="ACHOS5_003241"
     CDS             complement(2456..3997)
                     /gene="ppx"
                     /locus_tag="ACHOS5_003241"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP9778011.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFHGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4002..6068)
                     /gene="ppk1"
                     /locus_tag="ACHOS5_003242"
     CDS             complement(4002..6068)
                     /gene="ppk1"
                     /locus_tag="ACHOS5_003242"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP9778012.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6239..6877)
                     /gene="purN"
                     /locus_tag="ACHOS5_003243"
     CDS             complement(6239..6877)
                     /gene="purN"
                     /locus_tag="ACHOS5_003243"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9778013.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6877..7914)
                     /gene="purM"
                     /locus_tag="ACHOS5_003244"
     CDS             complement(6877..7914)
                     /gene="purM"
                     /locus_tag="ACHOS5_003244"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9778014.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8239..8865
                     /gene="upp"
                     /locus_tag="ACHOS5_003245"
     CDS             8239..8865
                     /gene="upp"
                     /locus_tag="ACHOS5_003245"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9778015.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8951..10240
                     /gene="uraA"
                     /locus_tag="ACHOS5_003246"
     CDS             8951..10240
                     /gene="uraA"
                     /locus_tag="ACHOS5_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP9778016.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10335..11036
                     /gene="hda"
                     /locus_tag="ACHOS5_003247"
     CDS             10335..11036
                     /gene="hda"
                     /locus_tag="ACHOS5_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP9778017.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11174..11533)
                     /gene="arsC"
                     /locus_tag="ACHOS5_003248"
     CDS             complement(11174..11533)
                     /gene="arsC"
                     /locus_tag="ACHOS5_003248"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP9778018.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIMG"
     gene            complement(11554..13017)
                     /gene="bepA"
                     /locus_tag="ACHOS5_003249"
     CDS             complement(11554..13017)
                     /gene="bepA"
                     /locus_tag="ACHOS5_003249"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP9778019.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPIVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13230..14291
                     /gene="yfgO"
                     /locus_tag="ACHOS5_003250"
     CDS             13230..14291
                     /gene="yfgO"
                     /locus_tag="ACHOS5_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9778020.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14390..14860)
                     /gene="bcp"
                     /locus_tag="ACHOS5_003251"
     CDS             complement(14390..14860)
                     /gene="bcp"
                     /locus_tag="ACHOS5_003251"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP9778021.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14860..15432)
                     /gene="gcvR"
                     /locus_tag="ACHOS5_003252"
     CDS             complement(14860..15432)
                     /gene="gcvR"
                     /locus_tag="ACHOS5_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP9778022.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15578..16456
                     /gene="dapA"
                     /locus_tag="ACHOS5_003253"
     CDS             15578..16456
                     /gene="dapA"
                     /locus_tag="ACHOS5_003253"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9778023.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16473..17507
                     /gene="bamC"
                     /locus_tag="ACHOS5_003254"
     CDS             16473..17507
                     /gene="bamC"
                     /locus_tag="ACHOS5_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP9778024.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17720..18433
                     /gene="purC"
                     /locus_tag="ACHOS5_003255"
     CDS             17720..18433
                     /gene="purC"
                     /locus_tag="ACHOS5_003255"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9778025.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18601..19464
                     /gene="ypfJ"
                     /locus_tag="ACHOS5_003256"
     CDS             18601..19464
                     /gene="ypfJ"
                     /locus_tag="ACHOS5_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP9778026.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWEHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19479..21494
                     /gene="tmcA"
                     /locus_tag="ACHOS5_003257"
     CDS             19479..21494
                     /gene="tmcA"
                     /locus_tag="ACHOS5_003257"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP9778027.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARQGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFAQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQRPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVRTERLRDRITQWQFFH"
     gene            21568..22266
                     /gene="ypfH"
                     /locus_tag="ACHOS5_003258"
     CDS             21568..22266
                     /gene="ypfH"
                     /locus_tag="ACHOS5_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9778028.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGRWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22347..22547)
                     /gene="ypfN"
                     /locus_tag="ACHOS5_003259"
     CDS             complement(22347..22547)
                     /gene="ypfN"
                     /locus_tag="ACHOS5_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP9778029.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22575..23702)
                     /gene="dapE"
                     /locus_tag="ACHOS5_003260"
     CDS             complement(22575..23702)
                     /gene="dapE"
                     /locus_tag="ACHOS5_003260"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP9778030.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFINELVAIE
                     WDQGNDFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23706..24062)
                     /gene="yffB"
                     /locus_tag="ACHOS5_003261"
     CDS             complement(23706..24062)
                     /gene="yffB"
                     /locus_tag="ACHOS5_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP9778031.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         5665 a         6021 c         6344 g         6162 t
ORIGIN      
        1 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
       61 tgacacttag aatcattgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
      121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
      181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
      241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgaag
      301 tttttctctt ttatctgcca ctggcactga tgatcagcat gatgatgttt ttcagctggg
      361 cggcattgcc agggatcgcc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
      421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
      481 acagggtttt tactccccgg cgtaacaacg tttcacatgg tgatagccgt ttaatttccc
      541 agcgactatt ctggcagatt gtgtttcctg caacgctgtt tctgatactt ttccagtttg
      601 ctgcgtttgt tggattactg gcgagcagag aaaatctggt cggcgtcatg ccctttaacc
      661 tcgggacctt aatcaattat caggccttgc tggtgggtaa tctgattggt gtcccgctgt
      721 gctacttcat cattcgggtg gtgcgaaatc cgttttattt acgtagctat tattcgcaat
      781 taaaacagca ggttgatgcc aaagttacca aaaaagagtt cgcaatctgg ctactggcat
      841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcacg atttttagca
      901 ccaattacac cttgtcatta ttgctgcccc tgatgatgtg gggagcgatg cgctatggtt
      961 ataagctgat ttcattgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
     1021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
     1081 tggtattctc ttttattgtc aattatatgg ctgtactggc aacccgtcag cgagcggtag
     1141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
     1201 ccctgaatcg cgcgttacgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
     1261 gcatggaaat gctggttaag aattatggca tcatgctgcg gattcaatac aagcaaaaac
     1321 tttctcactg gctgtcacca ttgttggaac cgggtgaaga tgtttatcag ctttcgggta
     1381 acgatctcgc gctgcggctg aatacagaat cgcaccagga gcgcattacc gcactggata
     1441 gccatctcaa gcaatttcgt ttcttttggg atggaatgcc gatgcaaccg cagattggcg
     1501 tcagttactg ctatgtgcgc tcgccagtga atcatatcta cctgctgctg ggagagctaa
     1561 atacggtggc cgaactttcc atcgtgacca acgccccgga aaatatgcag cgtcgcggag
     1621 caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcgg ctacagcggg
     1681 cgctggaaca caaccatttt ttcctgatgg cccagccgat taccggtatg cgtggtgatg
     1741 tttaccatga aattcttctg cgcatgaagg gtgagaatga tgagctgatc agccccgata
     1801 gcttcttacc ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg tgggtcattg
     1861 agcatacgct gcaatttatg gctgaaaaca gagcgaagat gcccgctcac cgttttgcta
     1921 ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc tgttgaagtc agtcagctgc
     1981 tggctaaata tcagattgaa gcgtggcaac ttatttttga agtcaccgaa agtaatgctc
     2041 tgaccaatgt taagcaggcg caaatcacct tgcagcatct tcaggaatta ggctgccaga
     2101 ttgcgattga tgatttcggc accggctacg ccagctatgc gcggcttaaa aatgtgaatg
     2161 ccgatctgct taaaattgac ggcagtttta tccgcaatat tgtatcaaat agtctggatt
     2221 atcagatagt ggcgtcgatt tgccacctag cgcgaatgaa gaaaatgcgg gtagtggcag
     2281 agtacgttga aaacgaagag atccgcgagg cggtgctctc tttggggatc gattatatgc
     2341 agggttatct tattggtaag ccgcaaccgt taattgatac gctgaatgaa atcgaaccca
     2401 ttcgcgaaag tgcctgaata atgcgggccg acatttctcg tcggcccgca aagcattaag
     2461 cggcgatttc tggtgtactt tcttcttcaa ttttcaaccg ccagccagcc acgccttccc
     2521 agtattcttg ctccttttcc agatcaagca gtaccagcgc attctgacta aaccagtcat
     2581 gcgggaaacg cagtgtccag tgactgtcat cagtaatcag cgtcaatgtt ggcggtgtgg
     2641 tggttgcctg acgttgatta ttgagtaata cgccaaggcg caatagctga atcagtggca
     2701 ggaactgttt cttcttaaac aaggtaaagc gcggcagatc gtcgagttta atcgctttac
     2761 ggtgatagcg caccagtgtc gccatcatca gctgctgttc ctgattaaaa cccggcaagt
     2821 cgctgttttg cagaatataa gcggagtggc gatgcaaacc gctgtggttg atattcaacc
     2881 cgacctcatg cagcatggcg gcccatcgca gtagcgcctc cagttgtgga tgcgccagct
     2941 tcggttgctg ttcccgccac tgttcgtaca tttgcatagt ggtatccagc acccgtcggg
     3001 cctgttcgct gtcgatgtga tactggttgg cgaggctgct ggcagtgcga ctacgcacat
     3061 cctgatgacg gaaacgtcct tccatttcat acagtacgcc ttcgcgtaac gccccgtcag
     3121 aaaggcgcag ttcacggatg gctaaagcat caaacacacc gcataaaatc gccagtccag
     3181 gaacgaagac tgttttccgc tcttcggaaa gacctggtaa actcagcgat gcgaaattac
     3241 ggtgccgtaa aacttctttt accagttttt ccagacgttc cggggtaatt atcccgtctt
     3301 tctcgcccat ttccattaac acttcatggg cggcttttat ggtaccggaa gcgcccattg
     3361 ccacgttcca gccctgaatt cgaaattgcc aggttaacgt ttccagtttt tgtgctgccg
     3421 ccatgcgagc gcgctgaaaa ttctctttat tgatgacccc gccatgaaaa taaagctggg
     3481 caaagctgac acaacccatc cggcggcttt caacgagaat cggttcgaaa ttttcaccaa
     3541 ttaccagttc tgtagaaccg ccgccaatat caataaccag tttgcgacct ttttccggtt
     3601 gggtatgttc cacgcccata aaaatcagac gggcttcttc attaccggaa ataatttcga
     3661 tcgggtaggg aatgactttt tccgcgcgtt tcagaaagtc agtggcgttc agcgcctgac
     3721 gcagcgtatg ggtaccaact atacagacgc tggcaggaga aaacccctgt agcctttcgg
     3781 caaatagcga cagacagttc aaaccgcgcg tcattgcctc ttcactcaac atattatctg
     3841 gccccagccc gtccgccaga tgcacccgct gtttcaggcg gccaataatc tgcatggcac
     3901 catctaccac acgggctatg accatgtgaa aactgtttga accaagatcg accgcagcaa
     3961 actcctgcgg acgaggggat ttatcgtgta ttggcatagg gttattcaga ttgttcgagt
     4021 gatttgatgt agtcgtaaat cgccaactgc gcccgtactt tgcggcgatt gccgcgggga
     4081 acgtagcgat tactgagttc tttatcgata taacgtgctt tgaccgtatc gctgaacaat
     4141 atgtcgatga tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg cgttgccact
     4201 tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta gaccttttta
     4261 tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa ggtaacggtc aacaatactg
     4321 atggcacgaa tgttgtcgct aatgccttcc agattgggga tcagagaaca cattccgcgc
     4381 accagcagat taaccggtac gccggagctg gaggccgcat acagacgatc aaccaggcct
     4441 ttatcgacaa ggttatttag cttcagggtg ataccactgg gcagcccttg ctgcgcgttg
     4501 gcgatctcgc ggtccaccat ttcatacaac agacggcgag agttttgcgg cgacaccatt
     4561 aaataatcaa atgtcaccgg acggtatggg ttttcaataa agttgaacac ccgccgtact
     4621 tcgttagtga tgcgcgcatc ggcggtcagc aacgaatagt cagtataaag acgcgcggtt
     4681 ttttcgttaa agttcccggt cccgatgtgt gcgtaacgca ccacttcacc gttttcttta
     4741 cgtgaaatca ggaacagttt ggcgtgaatt ttcagtcccg gcgcagagaa gataacgtgc
     4801 acgccagctt cggtcaggcg cttcgcccag tgaatgttgg cttcttcgtc gaaacgcgcc
     4861 tgtaactcaa ccaccacggt gactttctta ccgttgtgtg cggcgtggat catcgagtcg
     4921 atgatgcgtg aatctttcgc cacgcggtaa atgttaattt taatcgccag tacgctcggg
     4981 tcgaacgacg cctgacgcag cagttccagc acatgctcaa aggtgtgata aggataatag
     5041 agcaacacat cgcgttcgcg aatggcatca aaaccattgc ggaactgggc tttatcaaac
     5101 caaatatggc gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc gacattgggg
     5161 aaattaataa agtctttaaa attatgataa cgaccgccgg ggacgatgga gtcgtagcgg
     5221 gaaatagtca gtttttcgcg taacacttca accagtgcat tgggcatatc gcgctgataa
     5281 acaaaacgca ccggctcagc agttaaacgc tgcttgagac tggaagacat caactccatc
     5341 agactggctt ccatctcatg cactaaatcg tattcggcat cgcgggtcat cttcattgaa
     5401 taggcattca gtgcgtcata atcaaagaag cctttgaaaa tatcatcaag gcagtaacgc
     5461 agaatgttat ccagaagaat catcggcttg cgacgacgcg gcgcttctgg cggtaaattc
     5521 acaaagcgcg gcactttatc tgatgggatc tccagcagcg cgtaacggat ggtatcgcca
     5581 cggataattt ccaccgccag ataggtgtaa tcatctttca ggaactgcac taagtcagtg
     5641 tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat actgcttaaa ataatgacgc
     5701 agccagtttt gttgattgac ggagagctgg cgttcattaa tcaggaagat ctggttgcgc
     5761 gccatttcca gcagtagctc gttgtagagg ccgtcgaatt cctgatcggc tttcagcaca
     5821 cgggactgaa ttttgcccag taaatggcgg gaatgagagt tagagccttg ttcttcgcta
     5881 ataatgatgc gtcgcttcag ttcagcgaag cggactttat agaactcatc aaggttattg
     5941 gaatagatcc ccaggaaacg catcctttca atcagcgggt tagatttgtc cgccgcttcc
     6001 tgaagcacgc gttcattgaa cgataaccaa ctgagctctt tttcgatgta tagcttttcc
     6061 tgacccatta ccacttttac tccgttttat tcacgggaca ctgcctggat attatggcga
     6121 gcaatattgg cagatgtcca actatgccag aaaaggggga aaaaaactgc tggagcttgc
     6181 gatttacagg tgaattgaaa aacgccagcg gcagagctgg cgctttaact acggggcatt
     6241 actcgtcggc agcgtagccc tgcggcggca gacgttgacc atccagccac gcggcgtttt
     6301 cgtgcatttt cagacgacca tcggcaaacc agctaatcac cagtggataa atagcgtgtt
     6361 cctgggtttg cacgcgggcg gtgatgtcat cttccgtatc accagcaaat accgggactt
     6421 tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt gacgaaatgc accgatgtac
     6481 cgtgctcctc atcgccattt tccagcgcct gacgatgggt gtgtaatccg ggatatttcg
     6541 gcagcagaga agggtgaatg ttcagcaaac gcccggcata gtgggagaca aacgccgggc
     6601 tgagaatgcg cataaaacca gccagcacga ccacatcggg tgcgtacatg tcgatttcat
     6661 gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc gctggtgatg agcgtatgcg
     6721 ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta ttgctgaaaa
     6781 ctgcccgtac ggtgccttta attttgttgg ttttgcaggc gtcaataatt gcctgcaaat
     6841 tacttccgtt gccggaaata agcaccacaa tattcattat tcgataacca cgcgttgttc
     6901 ggaatcagag gctttgatga taccgatttt ccacgcgttt tcaccgttgg cattgagcag
     6961 ggcgagggct ttgtccactt ccggagcagg cagggcaata atcataccga cgccgcagtt
     7021 gaaggtgcga tacatttcat ggcgctcaac gttacctgcc gtttgcagcc agttgaacac
     7081 ttccggccac tgccaggaag attcatcaat cactgcctgg gtattatctg gcaatacgcg
     7141 cggaatgttt tcccagaagc cgccgccggt caggtgcgca atggcatgca catcgacctt
     7201 ttcaatcaac tccagcactg acttcacgta aatgcgggtc ggtgccagca gatgatcggc
     7261 taatggctta ccatcaagtt cggtggtttg cggatcacaa ccgctgactt caagaatttt
     7321 gcgcaccagc gaatagccat tcgagtgcgg gccgctagag ccaagtgcaa tcagcacatc
     7381 gccgtcgctg actttagatc cgtcgatgat ttctgatttt tctaccacgc caacgcagaa
     7441 accagcgaca tcgtaatctt caccgtgata catccccggc atttccgccg tttcgccacc
     7501 caccagtgaa cagcccgatt gcagacaacc ttccgcaatg ccgctgatca ccgctgaagc
     7561 ggtatcaaca tccagttttc cggttgcgta atagtcgagg aaaaacagcg gttccgcacc
     7621 ttgcaccacc aggtcattaa cgcacatggc gaccagatca ataccaatgg tgtcgtgacg
     7681 ttttaagtcc atcgccagac gcagcttggt acctacgccg tcagtgccgg aaaccagcac
     7741 aggttcacga tatttttgcg gcaatgcaca cagcgcaccg aagccgccca gaccgcccat
     7801 cacttccgga cgacgcgttt tcttcactac gcctttgatt cttccaacca gagcattacc
     7861 cgcgtcaata tcaacaccgg catctttgta gctaagagag gttttatcgg tcactgcttg
     7921 ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta acagggaaag
     7981 caaacgtttg cgagactgct ttacacaacc tttttgcacg tcttttcccc aggcgcgcgg
     8041 cgaaagaata cttgtaccag ggcaaaggat cgttttcgga tggaataatc ttctttcata
     8101 accatttgaa tataaaataa ctttatctca aaccgttatc gttttgacta aagtcaacga
     8161 aaataatatt gccgccttga agaaaggagg tataatccgt cgattttttt tgtggctgcc
     8221 cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac acccactcgt caaacacaag
     8281 ctgggactga tgcgtgagca agatatcagc accaagcgct ttcgcgaact cgcttccgaa
     8341 gtgggtagcc tgctgactta cgaagcgacc gccgacctcg aaacggaaaa agtcactatc
     8401 gaaggctgga acggcccggt agaaatcgac cagatcaaag gtaagaaaat taccgttgtg
     8461 ccaattctgc gtgcgggtct tgggatgatg gacggtgtgc tggaaaacgt tccgagtgcg
     8521 cgcatcagtg tcgtcggtat gtaccgtaat gaagaaacgc tggagccggt accgtacttc
     8581 cagaaactgg tttctaacat cgatgagcgt atggcgctga tcgttgaccc aatgctggca
     8641 accggtggtt ccgttatcgc gaccatcgac ctgctgaaaa aagcgggctg cagcagcatc
     8701 aaagttctgg tgctggtagc tgcaccagaa ggtatcgctg cgctggaaaa agcgcacccg
     8761 gacgtcgaac tgtatactgc atcgattgac cagggactga acgagcacgg atacattatt
     8821 ccgggcctcg gcgatgccgg tgacaaaatc tttggtacga aataaagaat aaaaataatt
     8881 aaagccgact ttaagagtcg gctttttttt gagtaaagcg cctataacac ataatacaga
     8941 ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa agaccgccac ttttacagac
     9001 aatcccgctt agtttgcaac atttgttcgc catgtttggt gcaaccgtcc tggtgcccgt
     9061 cttatttcat attaacccgg cgactgtact gttatttaac ggtataggaa cactgctgta
     9121 tctcttcatc tgtaaaggga aaattccggc ttatcttggt tccagctttg cctttatttc
     9181 accggtattg ttactgttgc cgttagggta tgaagtcgcg ctgggcggct ttattatgtg
     9241 cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa gcggggaccg gctggctgga
     9301 cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc gtcatcggtc tggagctggc
     9361 gggcgtagct gccggtatgg cgggtttact cccggctgaa gggcaaacgc cagactccaa
     9421 aaccatcatc atctctatta ccaccctggc ggtcacggtt ttaggttccg tgctgtttcg
     9481 tggtttcctg gcaattatcc cgattttaat tggcgtgctg gtggggtacg cgctctcttt
     9541 cgcaatggga attgtcgata ccacgccgat tattaatgct cactggtttg cgctgccgac
     9601 cctctatacg ccgcgcttcg agtggtttgc cattctgact attctgccag cggcgttagt
     9661 ggttattgcc gaacacgtag ggcacctggt cgtaacggct aatatcgtca aaaaagatct
     9721 gctgcgcgat ccaggtctgc accgttcgat gtttgctaac ggcttgtcga ccgtgatctc
     9781 cggcttcttt ggctccacgc caaacaccac atacggagaa aacattggcg tgatggcgat
     9841 cacccgtgtt tacagtacct gggttatcgg cggtgcggca attttcgcta tcctgctttc
     9901 ctgcgtcggt aaactggctg cagctatcca gatgattcca ttgccggtga tgggcggcgt
     9961 ttcgctgctg ctttacggtg tcatcggtgc ttccggtatt cgtgtgttga tcgaatcgaa
    10021 agtggattac aacaaagcac agaacctgat cctgacttcc gttattttga tcatcggcgt
    10081 cagtggcgcg aaggtaaaca ttggcgcggc ggagctgaaa ggtatggcgc tggcgaccat
    10141 cgtcggtatc ggcttaagtt tgatcttcaa actgattagt atgctgcgcc ctgaagaagt
    10201 ggtgctggac gcagaagacg cggatataac agacaaataa tgtaccgacc gggcagtgtt
    10261 gctgcccggt tcaaacatca tgggattctg tggtaaactt ctcgcgattt tgtgaaatcc
    10321 tggttgaggt atctctgaac acaccggcac agctctcttt gccactttat cttcctgacg
    10381 acgaaacctt tgcaagtttc tggccggggg ataactcctc tttactggcc gcgctgcaaa
    10441 acgtgctgcg tcaggaacat agcggttaca tctatctctg ggcacgcgaa ggcgcggggc
    10501 gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca gcgtggcgat gcggtgggct
    10561 atgtcccgct ggataaacgc acctggtttg ttccggaagt gctcgacggt atggagcatt
    10621 tgtcgctggt ctgtattgac aacattgagt gtattgccgg cgatgagttg tgggagatgg
    10681 cgattttcga tctctacaat cgaattctgg aatcgggcaa aacacggttg ttgatcaccg
    10741 gcgatcgtcc accgcggcag ttgaatctgg gattaccgga tctcgcgtcg cgtctcgact
    10801 gggggcagat ctacaaattg cagccacttt ctgatgaaga taagttgcag gcgctacagt
    10861 tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt ggggcgtttc ttgctgaagc
    10921 ggctcgacag agaaatgcgc acgttgttta tgacgctgga tcagctggat cgtgcgtcga
    10981 ttaccgcgca acgcaagctg accattcctt ttgtgaaaga aattctgaag ttgtagataa
    11041 ggtgtttatt gtcggatgcg gcgcgaacgc cttatccgac ctacgcgaag ttaatccata
    11101 gtattcgtag gccggataag acgcggcaag cgtcgcatcc ggcaatgtgc ttcaactagc
    11161 gatggagcac atcttaaccc atgatttcca gtacctgctc cggtggacga ccaatccgtg
    11221 ctttgccatt cgcgaccaca atcgggcgtt ccattagctt cggattatcc accatcgcct
    11281 gaatcagcgc ctcttcgcta agtgaactgt cagccaggtt cagttcttta taaagatcct
    11341 ctttttggcg catcaattca cgagcgctat tcatccccag cattttcagc aaatcacgca
    11401 gcgttgccgc atcggcgggt gtctcaaggt agagcaccac ttctggctcc acgccgtttt
    11461 ctttcagcag gttcagcgtt tcccggctct ttgaacagcg tgggttatgg tagattttta
    11521 cctgtttggt cattgttctt cctttaatgc gaattacatc ttggtataag gcttaaagcg
    11581 ttcctgcagc tggcgcaact ggtcgatgcg cgcatcgtaa cgcgcttgtt gcaggctgcc
    11641 taatttcacc tgagaactgg cgctactcaa caaggaaata gcctgatcga gtcgcccggc
    11701 gagcgcataa ccttctgctc gcgcagccag ttcctgatcg cggttattta gtgcggcttc
    11761 cgcctgtgcc agtaaatccc agccgttgct gtcatcttta ttgttaaaag tgtagcgatt
    11821 cagaatattg gccgcttcct gtggttgccc cccttgcaga taagcgttcg ccaggttgag
    11881 ctgcaacacc ggattggtgc gcaaatcgcg ggcatttttc aggcgattga tagcgtcatt
    11941 ggctttgttt tgcccaagat cgatatcggt agccagatcg agataccatg cgttgccagg
    12001 ttctgccgcc agtaacggtt gcagcgtttt acgcgcttcg tcgtatttat tggcttccat
    12061 cgcctgtaga gcacgaccat attgcgccgc tcgttgctga cgaacgtttc ctttcgccca
    12121 ttcatccagc aaatcactgg tgagctggtt acgtccggaa ttatacatcc ccagtgtgcg
    12181 cactttcgcc agatagaaat cttctgacga ttgcaccact atcgggcgca tctgattggc
    12241 acggttacgg gcatcagcca gacggctttc cggtagtggg tgagtgagca gaatttctgg
    12301 tgggcgcgag gagtaacgcg cctgatcgag taatttttcg aggaaggtcg gcatcgcctg
    12361 cggatcgaat cccgagcgtt gcagcacctg aataccaatg cggtccgctt cctgttcatt
    12421 ttgctgggtg aaactgatca tcccctggcg cgttcccgcc agtgtaccgg tcagcgccgc
    12481 catccccgcc tgcggactgg ccatcgccag taaaatagaa cctaacgcgc cgacccaggt
    12541 cagcggcgcg ctgcgctgct gatcttccat cgctcgcgcc aggtgacgtt gggtgacgtg
    12601 ggagatttcg tgcgccataa ctgaagccag ttgactttcg ttatcggaat aacggaacag
    12661 ggcagagtgc agcaccacgt tgccgccaaa gaaagcaaag gcgttaattt cgtcgttgtt
    12721 gatcagaaag aaatgaaacg gtgtcttaac cgaattggca tgcgaaacca gacgcattcc
    12781 tagcgaatta atgtattgcg ttaacagcgg atcattaatt aacggcgcgc tgccgcgtaa
    12841 ctggcggaca taatagtcgc ccatctgcat ttcctgacca atggaaagcg tgcttcctgc
    12901 ggaggttccc atatccggca aggtgtctgc gctgtcggca aacgccgggg ctacctgacc
    12961 aatggtcata gcagcaatga gggttgcaac caggtttttt ttcaactgcc tgaacataac
    13021 ctctatcctg tatttctgga gatagtcatt tgaccgatac tgtgaaatat cgttcccacc
    13081 gcgccgcctt tgagtgtaac ggcgaagatt agcccactca aatgacttct cagtttcctg
    13141 aatccgtcat aaaaagcgaa gtcttttttg tgacatttcg atacaattgc ggatcgcaat
    13201 cccgctcttg aggttcaggg aaggtctgta tgctcgaaat gttgatgcaa tggtatcgcc
    13261 gccgttttag cgacccggaa gcgattgcct tgctggttat tttggttgct ggttttggca
    13321 ttatcttttt ctttagcggc ctactcgcgc cgttgctggt ggctattgtg ctggcctatt
    13381 tgctggaatg gccgactgtc cgcctgcagt ctattggctg ttcccgccct tgggcgacat
    13441 ctatcgtgct gatcctgttt gtgggcatcc tgctactgat ggcgttcgtg gttctgccta
    13501 ttgcctggca gcagggcatc tacttaatcc gcgatatgcc agggatgctt aataaacttt
    13561 ctgattttgc tgccacgtta ccgcgccgct atccggcatt aatggatgcg gggattattg
    13621 atgcaatggc tgaaaatatg cgtagccgga tgctgaccat gggcgattcg gtggtgaaaa
    13681 tttccctcgc ctcgttggtc ggtttgctga ccattgccgt ctatctggtg ctggtgccat
    13741 tgatggtctt cttcctgctg aaagacaaag agcagatgct gaacgccgtt cgccgggtgc
    13801 tgccgcgcaa ccgtggactg gcaggacagg tgtggaagga gatgaatcaa caaatcacca
    13861 actatatccg cggcaaagtg ctggagatga tcgtggtagg gatcgccacc tggctggggt
    13921 tcttgctctt tgggctgaac tattcgctgc tgctggcggt gctggtcggc ttctcggtac
    13981 ttattccgta cattggcgca tttgtggtga ccattccggt ggttggcgtg gcgctattcc
    14041 agtttggtgc tggcacggaa ttctggagct gcttcgcggt gtatctgatt attcaggcgc
    14101 tggacggcaa cctgttagta ccggtgttgt tttccgaagc ggttaacctg catcctctgg
    14161 tgattatttt atcggtggtg atcttcggtg gtttgtgggg attctggggc gtattcttcg
    14221 ctattccatt ggcgacgctg atcaaagccg tgattcacgc ctggcctgat gggcaaatcg
    14281 cgcaagactg accttcagcg aaaaaatttg aaacgagtgt tgcagggcag tgagaagatt
    14341 tgagatgtgc tggccgcaag cgcagccagc acggaatgga gcaaagtaat caggcgtgtt
    14401 ctttcagcca gttcagcaca acgtcgtggt gattgctggt tttgaaatcg tcaaaaacat
    14461 gttcgatttt gccatcagcg tcaatcagga agctgatgcg atgaatacca tcgtaggttt
    14521 tgcccatgaa ggacttttca ccccagacgc cgaattgttc gcacacctgg tggtcctcat
    14581 cagacaggag cgtaaagtta agcagctctt tttccgcaaa acgggagagt ttttcgggtt
    14641 tatcggtgct gatacccagc acatcaacgc ccgctttttt caactcatcc atgttatcgc
    14701 gtaagccgca ggcctgtacg gtacagccgg gggtcatggc tttcgggtag aaataaacca
    14761 gaacacgctg tccctggaag tcggtcaaat taacttgttc tccgtcttga tccggcaagc
    14821 taaatttcgg tgcgatatca ccggctttca gtggattcat tacttaactc catcctgttc
    14881 atcatgttgg gaataattga cgacgttaat actgccttgt gcattgagtt ctgtacatag
    14941 ggctttgaac gcttgctcaa tatttgctgc gtccgcagat gcggggctgt gggcagttat
    15001 ctgaatatgc aactgcgcag ccctttcatt ttcagcaggt tgcgtgcgcg acaccagctc
    15061 cgcaatgttc atatgatgcg cgtcgaaaag tgctgtgaag cgttcaatta aatgcgggga
    15121 gtctgccaca tcgacctgaa cccagacaga tgctggcatt ggcggacgcg gacgcgccgt
    15181 cgtgcgcttc atcacgatta aaagatccag ctcggcacct ttcaacggta acgttgattc
    15241 aatcagagta atggcattcc atgaaccgga aagcagcatg ataaacgtga actcttctcc
    15301 cagcatcgcc aggcgactgt cttcaatatt acagccgcaa ctactgacat gacgggtgat
    15361 ggtgttcact attccagggc gatcggcacc caacgcagtg atcaccagat aatgttgcga
    15421 tgacagtgtc aaactggtta ttccttcaag gggtgagttg ttcttaagga aagcataaaa
    15481 aaaacatgca tacaacaatc agaacggttc tgtctgcttg cttttaatgc cataccaaac
    15541 gtaccattga gacacttgtt tgcacagagg atggcccatg ttcacgggaa gtattgtcgc
    15601 gattgttact ccgatggatg aaaaaggtaa tgtctgtcgg gctagcttga aaaaactgat
    15661 tgattatcat gtcgccagcg gtacttcggc gatcgtttct gttggcacca ctggcgagtc
    15721 cgctacctta aatcatgacg aacatgctga tgtggtgatg atgacgctgg agctggctga
    15781 cgggcgcatt ccggtgattg ccgggaccgg tgctaacgct actgcggaag ccattagcct
    15841 gacgcagcgc ttcaatgaca gcggtatcgt cggctgcctg acggtaaccc cttactacaa
    15901 tcgtccgtcg caagaaggtt tgtatcagca tttcaaagcc atcgctgaac atactgacct
    15961 gccgcaaatt ctgtataatg tgccgtcccg tactggctgc gatctgctcc cggaaacggt
    16021 gggccgtctg gcgaaagtaa aaaatattat cggaatcaaa gaggcaacag ggaacttaac
    16081 gcgtgtaaac cagatcaaag agctggtttc agatgatttt gttctgctga gcggcgatga
    16141 tgcgagcgcg ctggacttca tgcaattagg cggtcatggg gttatttccg ttacggctaa
    16201 cgtcgcagcg cgtgatatgg cccagatgtg caaactggca gcagaagggc attttgccga
    16261 ggcacgcgtt attaatcagc gtctgatgcc attacacaac aaactatttg tcgaacccaa
    16321 tccaatcccg gtgaaatggg catgtaagga actgggtctt gtggcgaccg atacgctgcg
    16381 cctgccaatg acaccaatca ccgacagtgg tcgtgagacg gtcagagcgg cgcttaagca
    16441 tgccggtttg ctgtaaagtt tagggagatt tgatggctta ctctgttcaa aagtcgcgcc
    16501 tggcaaaggt tgcgggtgtt tcgcttgttt tattactcgc tgcctgtagt tctgactcac
    16561 gctataagcg tcaggtcagt ggtgatgaag cctacctgga agcggcaccg cttgcggagc
    16621 ttcatgcccc ggctggaatg atcttgccgg tgacctccgg cgattatgca atcccggtaa
    16681 ccaacggtag tggtgccgtc ggtaaggcgc tggacattcg tccaccagcc cagccgctgg
    16741 cactggtttc tggcgcgcgt acccagttca cgggcgatac cgcttcattg ctggtggaaa
    16801 atggtcgtgg caataccctg tggccgcagg tggttagcgt gctgcaggcg aaaaactaca
    16861 ccatcaccca acgtgatgat gctggtcaga cactgaccac cgattgggta caatggaacc
    16921 gtctggacga agacgagcag tatcgtggtc gttatcaaat ctctgttaag ccgcagggtt
    16981 atcagcaggc ggttacggtt aaactgctga acctggaaca ggcgggcaaa ccggttgcag
    17041 acgcggcttc catgcagcgt tacagcacgg agatgatgaa cgttatttcc gccggtctgg
    17101 ataaatctgc cactgacgcc gcgaacgctg cgcaaaatcg tgcctccacc actatggacg
    17161 tacaaagtgc agctgatgac accggtttac caatgctggt cgtacgcggg ccgttcaatg
    17221 tggtttggca acgtctgcca gctgcgctgg aaaaagtggg catgaaagtg actgacagca
    17281 cccgttcgca gggcaacatg gccgtaactt ataagccgct gtctgacagc gaatggcacg
    17341 aactgggcgc gagcgatcca ggtctggcat ccggtgacta taaactgcag gttggcgatt
    17401 tagataaccg cagcagccta cagttcatcg atccgaaagg tcatactctg actcagagtc
    17461 agaacgacgc gctggtagct gtcttccagg ctgcgtttag caagtaaaaa tacagggctg
    17521 gagtcatcca gccctttttt ctgatatgat acgcaaacgt gtgcgtcggc aggaaaacga
    17581 gattttagcg gtaattcaca cgaaatttgt ttgtcggacg tcgttcggat aaagcgttca
    17641 cgccgcatcc gacaaaacat ccggcacacc agacagcaaa agattttaaa acgttaattc
    17701 acacccagga gtgataaaga tgcaaaagca agctgagttg tatcgtggta aagcgaaaac
    17761 cgtatacagc acggaaaacc cggacctgtt ggtgctcgaa ttccgcaatg atacgtcagc
    17821 aggggatggc gcgcgcattg agcagtttga tcgcaaaggt atggtgaaca acaagttcaa
    17881 ctacttcatt atgagcaaac tggctgaagc gggtatcccg actcaaatgg agcgtctgct
    17941 ctccgatacc gaatgtctgg tgaaaaagct ggatatggtg ccggttgagt gtgtcgtgcg
    18001 taaccgtgct gctggctctc tggtgaaacg tcttggaatc gaagaaggta ttgagctgaa
    18061 cccgccgctg ttcgatctgt tcctgaaaaa cgacgccatg cacgatccga tggtcaacga
    18121 atcttactgc gaaacctttg gctgggtgag caaagagaac ctggcgcgta tgaaagagct
    18181 gacctacaaa gcgaacgacg tgctgaaaaa actgttcgat gatgctggtc tgattctggt
    18241 cgacttcaag ctggagtttg gtctgtacaa aggcgaagtg gttctgggcg atgagttctc
    18301 cccggacggc agccgcctgt gggacaaaga aacgctggag aaaatggata aagaccgttt
    18361 ccgccagagc ctcggtggcc tgatcgaagc ctatgaagcc gttgcccgcc gcctgggtgt
    18421 acagctggac tgatttttct attcatcatc ttgccgtgct actggcacgg caagacaacc
    18481 gctctcataa gatgtgcatt gaacgctcat tcctccgcca tttcatcccg ctttcctgtg
    18541 gtaatcttcc tctgaaccac ctacgatcat ttctataatg aatataatgt tgaggtatct
    18601 atgcgttggc aagggcgacg tgaaagtgac aatgttgaag acaggcgcaa cagctctggt
    18661 ggtccgtcta tgggcggtcc cggttttcgc ctgccaagcg gtaaaggcgg gctgatttta
    18721 ctgatagtcg tgctggttgc aggctactat ggcgttgatt taaccgggtt gatgaccgga
    18781 cagccggttt cccaacagca atcaacgcgg tcaattagcc ctaatgaaga cgaagccgca
    18841 aaattcacct cggtgattct ggcaaccacg gaagacacct ggggacagca attcgagaag
    18901 atgggtaaaa cctatcagca accgaagctg gtcatgtacc gtggaatgac gcgtaccggc
    18961 tgcggggcgg gccagtccat aatggggcca ttctattgcc cggcggatgg cacagtttat
    19021 atcgatctct ccttctatga tgacatgaaa gacaaacttg gcgcggatgg cgattttgcc
    19081 caggggtacg ttatcgctca tgaagtcggt catcatgtgc agaaactgct aggcatcgag
    19141 ccgaaagttc gtcaactgca acaaaacgcg acgcaggcgg aagtaaaccg cttatctgtg
    19201 cgtatggaac tccaggccga ctgttttgcc ggtgtatggg agcatagtat gcagcagcaa
    19261 ggtgttctgg aaaccggcga tctggaagag gcgctgaacg cggcgcaggc catcggcgat
    19321 gaccgtttac aacagcaaag tcaggggcga gtagtaccag acagtttcac tcatggcact
    19381 tctcagcaac gctatagctg gtttaaacgt ggtttcgaca gcggcgatcc ggcacaatgc
    19441 aatacttttg gtaaaagcat ttaacttccg gggcagggat ggctgaactg actgcgcttc
    19501 acacattaac agcgcaaatg aaacgtgaag ggatccgccg cttgctggtg ttgagcgggg
    19561 aagagggttg gtgttttgat catgcgctta agttgcgtga tgccttacct ggcgactggc
    19621 tgtggatttc gccgcagcca gatgctgaaa accactgttc accctcggcg ctacaaactt
    19681 tacttgggcg cgagttccgg catgcggtat tcgacgcccg ccaaggcttt gatgccgctg
    19741 cctttgcggc acttagcgga acgttgaaag cgggaagctg gctggtgttg ttactccctg
    19801 tatgggatga gtgggaaaac caacctgatg ccgactcgct gcgctggagt gattgccctg
    19861 accctattgc gacgccgcat tttgcccagc atttcaaacg cgtacttacg gcgaataacg
    19921 acgctatcct ctggcggcaa aaccagccgt tctcgttggc gcattttact ccccgtactg
    19981 actggcaccc cgctaccggc gcaccacaac cagaacaaca gcaactctta cagcagctac
    20041 tgaccatgcc gccgggcgtg gcagcggtaa ctgctgcgcg tgggcgcggt aagtcggcgt
    20101 tggcagggca actcatttct cgtattgcgg gcagtgcgat tgtcaccgcg cccgcaaaag
    20161 cggcaacgga tgtactggca caatttgcgg gtgagaagtt tcgctttatt gcaccggatg
    20221 ccttgttagc cagcgatgag caagccgact ggctggtggt cgatgaagcc gcagctatac
    20281 ctgcgccgtt gttgtatcaa ctggtatcgc gttttcctcg aacgttgtta accactacgg
    20341 tgcagggcta cgaaggcacc ggacgtggtt ttttgctgaa attttgcgct cgctttccgc
    20401 atttacaccg ttttgaactg caacggccga tccgctgggc gcagggatgc ccgctggaaa
    20461 aaatggttag tgaggcactg gtgtttgacg atgaaaactt cacccataca ccacaaggca
    20521 atatcgttat ttccgcattt gaacagacgt tatggcgaag cgagccagaa acgccgttaa
    20581 aggtttatca gttattgtct ggtgcgcact accggacttc gccgctggat ttacgccgga
    20641 tgatggatgc accagggcaa cattttttac aggcggctgg cgaaaacgag attgccgggg
    20701 cgctgtggct ggtggatgag ggcggattat ctcaacaact cagtcaggcg gtatgggcag
    20761 gttttcgtcg cccgcggggt aatctggtgg cccagtcgct ggcggcgcac ggcagcaatc
    20821 cactggcggc gacattgcgc ggaaggcggg tcagccgaat agcagttcat ccggctcgtc
    20881 agcgggaagg cacaggtcag caactgattg ctggtgcttt gcaatatacg caggacctcg
    20941 actatctttc ggtgagtttt ggttacaccg gggaattatg gcgtttctgg caacgctgcg
    21001 gttttgtgct ggtgcgaatg ggtaatcatc gtgaagccag cagcggttgc tatacggcga
    21061 tggcactgtt accgatgagt gatgcgggta aacagctggc tgaacgtgag cattaccgtt
    21121 tacgtcgcga tgcgcaagct ctcgcgcagt ggaatggcga aatgcttcct gttgatccac
    21181 taaacgatgc cgtcctttct gacgacgact ggcttgaact ggccggtttt gctttcgctc
    21241 atcgtccgct attaacgtcg ttaggttgct taatgcgtct gttacaaacc agcgaactgg
    21301 cattaccggc gctgcgtggg cgtttacaga aaaacgccag tgacgcgcag ttatgtacca
    21361 cacttaaact ttcaggccgt aagctgttac tggtccgtca acgcgaagaa gccgcgcagg
    21421 cgctattcgc acttgatgat gttcgcactg agcgtctgcg cgatcgcata acgcaatggc
    21481 aattttttca ctgactcctt caattttctc ccatggtcat tgtgagtcag cggcgtaaaa
    21541 ttaatgacat cagttaagga gttcgccatg aaacatgacc attttgttgt tcaaagcccg
    21601 gataaacctg cgcaacagtt gttactgctt ttccacggtg tcggggataa cccggtggca
    21661 atgggggaaa taggcagatg gtttgcgccg ctgtttcctg atgcgctggt ggtgagtgtc
    21721 ggaggcgcgg agccgagcgg taatccggcg gggcgtcagt ggttttcggt gcagggtatt
    21781 acggaagata atcgccaggc gcgtgtggat gccatcatgc cgacgtttat tgaaacggtg
    21841 cgctactggc agaaacagag cggagtaggg gctaacgcca cggcgctaat cggtttctca
    21901 caaggcgcga ttatggcgct ggagagcatt aaagccgaac cgggccttgc ttcgcgcgtc
    21961 attgccttca acggacgcta cgcgagcctg ccggaaaccg cgtctacagc cacgacaatt
    22021 cacttgattc atggcggtga agatccggtt atcgatctgg cgcatgcggt tgcggcgcag
    22081 gaagcgttaa tcagtgccgg tggcgatgtg acgctggata tcgtggagga tttaggccat
    22141 gcgatcgaca atcgcagtat gcaattagcc ctggatcact tgcgttatac cattccgaaa
    22201 cattatttcg acgaagcgct aagcggcggt aagcctggcg atgatgatgt gattgaaatg
    22261 atgtaataat agccgggtag gtctgataag actgtatcag gcatcatggc cggatgcggc
    22321 gttaacgcct tatccggcca cttagcttac ttcttcggtt gatccttttt cggccagtcg
    22381 tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg gaagctccgg cttgttggcg
    22441 agaaatttct tatggtcgac gcgcttgagg tctttaatca cgttcagcag gacgcctacc
    22501 aaaaagacaa tcaccagaat ccaccaatat ttagccagcc agtccatgct tatttcctct
    22561 tgcagaacca ctcatcaggc gacgagctgt tccatgatac gttgatacat acgggcaagt
    22621 agctgcaggt cggcagcgtt cacacattca ttaattttat gaatcgtggc attgaccggc
    22681 ccgagttcca ccacctgcgc ccccatgcgg gcaataaagc gcccgtcgga cgttccgcct
    22741 gtggtcagta gctgcggttt aatttcatta tagtgctcaa ccgcgttaac gaccgcatcc
    22801 accagtttac cgcgcgcggt caaaaatggc tgcccggaaa gccaccaatc caccgtatag
    22861 cgcagttgat gtttttcaag cagggcaagc acctgcgctt tgatcatctc atccgtcagt
    22921 tcggtgctaa agcggaagtt aaactgcaca aacagttcac ctggaataac gttgttactg
    22981 cccgttcccg cctgaatatt ggcaatctgc atactggtcg ccgggaagaa gtcattaccc
    23041 tgatcccact caatagccac taattcatta atgaaaggtg ctgcgcgatg taccggattg
    23101 tcagccagat gtgggtaggc aacatgcccc tgaacgccat gaatggtaag gttgcaggtt
    23161 aacgatccgc gacgaccatt tttcaccaca tcacctacca cttcgatact cgacggttcg
    23221 ccgaccaggc agtaatcgag acgctcatta cgtgccatta acgcttcgac gacttttacc
    23281 gtaccgttgt gggcactagc ttcttcatca gaggtgatca gaaatgccag tcgccctgta
    23341 tggttgggat gttgtgcgac aaagcgttct gccgctacca ccatcgccgc cagcgagcct
    23401 ttcatatctg ccgcaccgcg cccgaataac atgccatcac gaatggtggg ttcaaacggc
    23461 ggattgatcc aacggtcggc gtcgccaggc ggcaccacgt cggtatgccc ggcaaaggct
    23521 aacgtttcac cctgcccacg ccatgcccaa aaattctgcg tgtcggcaaa gtccatgcgt
    23581 tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca aagcctggca tcctgcgtca
    23641 tcaggactca gggaagggcg gcgaataagc tgttgtgtca gctcaataac cgggcacgac
    23701 atagactaca cctcatggaa aaattgctga taactggatt cactgaaacc cagcagcata
    23761 ggcttaccag gcgcgcagag caatggacgt ttgataattg caggcatttc agtcattaat
    23821 gtcgccgcag aggccgcatc ggtgatttta ttgcgggtgg tttcgtccag tttacgccag
    23881 gttgtaccac gggtgttgag taacgcttcc cagcctaatt cgttgataaa accgttcaat
    23941 aattcgctgt ccaatccatc gacgcggtaa tcatgaaaac gatagtcgat gttattagct
    24001 tccagccaac ggcgagcctt cttaatggtg tcacaatttt tgatgccgta aagtgtaacc
    24061 ataatgaatc ctttttcgat ttatgagaat aaatactcat ttaaaggtga aaacattttg
    24121 cctgcaaatg attatcagga aatataacat tatcgaagta ttcaaattaa caaccattaa
    24181 ataacattaa ac
//
LOCUS       DAZKHK010000083        23899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKHK010000083 DAZKHK010000000
VERSION     DAZKHK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.083"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            197..1828
                     /gene="oppA"
                     /locus_tag="ACHOS5_003262"
     CDS             197..1828
                     /gene="oppA"
                     /locus_tag="ACHOS5_003262"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP9778032.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            1914..2834
                     /gene="oppB"
                     /locus_tag="ACHOS5_003263"
     CDS             1914..2834
                     /gene="oppB"
                     /locus_tag="ACHOS5_003263"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP9778033.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2849..3757
                     /gene="oppC"
                     /locus_tag="ACHOS5_003264"
     CDS             2849..3757
                     /gene="oppC"
                     /locus_tag="ACHOS5_003264"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP9778034.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLV
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3769..4782
                     /gene="oppD"
                     /locus_tag="ACHOS5_003265"
     CDS             3769..4782
                     /gene="oppD"
                     /locus_tag="ACHOS5_003265"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP9778035.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4779..5783
                     /gene="oppF"
                     /locus_tag="ACHOS5_003266"
     CDS             4779..5783
                     /gene="oppF"
                     /locus_tag="ACHOS5_003266"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP9778036.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5836..6165)
                     /gene="yciU"
                     /locus_tag="ACHOS5_003267"
     CDS             complement(5836..6165)
                     /gene="yciU"
                     /locus_tag="ACHOS5_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP9778037.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6200..7660)
                     /gene="cls"
                     /locus_tag="ACHOS5_003268"
     CDS             complement(6200..7660)
                     /gene="cls"
                     /locus_tag="ACHOS5_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9778038.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7803..7976
                     /gene="yciY"
                     /locus_tag="ACHOS5_003269"
     CDS             7803..7976
                     /gene="yciY"
                     /locus_tag="ACHOS5_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP9778039.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8031..9284)
                     /gene="kch"
                     /locus_tag="ACHOS5_003270"
     CDS             complement(8031..9284)
                     /gene="kch"
                     /locus_tag="ACHOS5_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP9778040.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(9584..9880)
                     /gene="yciI"
                     /locus_tag="ACHOS5_003271"
     CDS             complement(9584..9880)
                     /gene="yciI"
                     /locus_tag="ACHOS5_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP9778041.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10104..10820
                     /gene="tonB"
                     /locus_tag="ACHOS5_003272"
     CDS             10104..10820
                     /gene="tonB"
                     /locus_tag="ACHOS5_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP9778042.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(10860..11258)
                     /gene="yciA"
                     /locus_tag="ACHOS5_003273"
     CDS             complement(10860..11258)
                     /gene="yciA"
                     /locus_tag="ACHOS5_003273"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP9778043.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11364..11903)
                     /gene="yciB"
                     /locus_tag="ACHOS5_003274"
     CDS             complement(11364..11903)
                     /gene="yciB"
                     /locus_tag="ACHOS5_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP9778044.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(11933..12676)
                     /gene="yciC"
                     /locus_tag="ACHOS5_003275"
     CDS             complement(11933..12676)
                     /gene="yciC"
                     /locus_tag="ACHOS5_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP9778045.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12704..12793)
                     /locus_tag="ACHOS5_003276"
                     /pseudo
     CDS             complement(12704..12793)
                     /locus_tag="ACHOS5_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13031..13684
                     /gene="ompW"
                     /locus_tag="ACHOS5_003277"
     CDS             13031..13684
                     /gene="ompW"
                     /locus_tag="ACHOS5_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP9778046.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKVGTATTGDIATVHHLPP
                     TLMAQWYFGDSGSKFRPYIGAGINYTTFFNNDFNDTGEGLGLSDLSLKDSWGVAGQVG
                     LDYLINRDWLLNMSVWYMDIDTEVKYKSNGAVLPAGRYSDNVRLDPWVFMFSAGYRF"
     gene            complement(13735..14541)
                     /gene="trpA"
                     /locus_tag="ACHOS5_003278"
     CDS             complement(13735..14541)
                     /gene="trpA"
                     /locus_tag="ACHOS5_003278"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP9778047.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEVLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRR"
     gene            complement(14541..15734)
                     /gene="trpB"
                     /locus_tag="ACHOS5_003279"
     CDS             complement(14541..15734)
                     /gene="trpB"
                     /locus_tag="ACHOS5_003279"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP9778048.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(15746..17107)
                     /gene="trpCF"
                     /locus_tag="ACHOS5_003280"
     CDS             complement(15746..17107)
                     /gene="trpCF"
                     /locus_tag="ACHOS5_003280"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP9778049.1"
                     /translation="MMQTVLAKIVADKAIWVETRKEQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVADKAKVLSLAAVQL
                     HGNEDQLYIDNLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(17108..18703)
                     /gene="trpD"
                     /locus_tag="ACHOS5_003281"
     CDS             complement(17108..18703)
                     /gene="trpD"
                     /locus_tag="ACHOS5_003281"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP9778050.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(18703..20265)
                     /gene="trpE"
                     /locus_tag="ACHOS5_003282"
     CDS             complement(18703..20265)
                     /gene="trpE"
                     /locus_tag="ACHOS5_003282"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP9778051.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFASNEDEKQRLTARLAELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(20287..20380)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(20357..20401)
                     /gene="trpL"
                     /locus_tag="ACHOS5_003283"
     CDS             complement(20357..20401)
                     /gene="trpL"
                     /locus_tag="ACHOS5_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP9778052.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            20539..21420
                     /gene="rnm"
                     /locus_tag="ACHOS5_003284"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             20539..21420
                     /gene="rnm"
                     /locus_tag="ACHOS5_003284"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP9778053.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            21417..22037
                     /gene="yciO"
                     /locus_tag="ACHOS5_003285"
     CDS             21417..22037
                     /gene="yciO"
                     /locus_tag="ACHOS5_003285"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9778054.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRHLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            22065..>23899
                     /gene="yciQ"
                     /locus_tag="ACHOS5_003286"
     CDS             22065..>23899
                     /gene="yciQ"
                     /locus_tag="ACHOS5_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFP9778055.1"
                     /translation="MAGIYRCILLLIVGLSFSSLSYAKNTEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMLPNGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNNWPF
                     EIYKTRFSLQLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTIL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVFPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLKDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEELFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGI
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAICAPEITRNGVLFSLQMHLSLGANTSLLGR"
BASE COUNT         6018 a         5879 c         6072 g         5930 t
ORIGIN      
        1 tctcgacagg ggagacacag tacgaatcga cataaggtga tcgtctgaat caccagaata
       61 aataaagtcg gtgatagtaa tacgtaacga taaagtaacc tgacagcaga aagtctccga
      121 gcctgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta attataatga
      181 gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag ctggcgtttt
      241 ggctgcgctt atggcaggga atgtcgcgct ggcagctgat gtacccgcag gtgtcacact
      301 ggcggaaaaa caaacgctgg tacgtaacaa tggttcagaa gttcagtcat tagatccgca
      361 caaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag gcttactggt
      421 cagcgatctt gacggtcatc cggcacctgg cgtcgctgaa tcatgggata ataaagacgc
      481 gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca cgccagttac
      541 agcacaagac tttgtgtata gctggcaacg ttctgttgat ccaaacactg cttctccgta
      601 tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg aagggaaaaa
      661 accgattacc gatctcggcg tgaaagctat tgatgatcac acattagaag tcaccttaag
      721 tgaacctgtt ccgtacttct ataaattact tgttcaccca tcaacttcac cggtgccaaa
      781 agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatcg tcaccaacgg
      841 tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac gcagcccgac
      901 ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta ttgcttctga
      961 agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacttata acaacatgcc
     1021 gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg ttgatccata
     1081 cctgtgcact tactattacg aaattaacaa ccagaaaccg ccatttaatg acgtgcgtgt
     1141 gcgtaccgca ctgaaattgg gtatggaccg cgatatcatt gttaataaag tgaaagcgca
     1201 gggcgacatg cccgcctatg gttacactcc accatatact gatggcgcaa aattgactca
     1261 gccggaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa aactgctggc
     1321 tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgttgtata acacctccga
     1381 tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca ttggtgtaaa
     1441 cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc agggcacttt
     1501 tgatgtggcc cgtgcaggct ggtgtgcaga ctacaacgaa ccaacttcct tcctgaacac
     1561 catgctatcg aatagctcaa tgaataccgc gcattataag agcccggcct ttgacagcat
     1621 tatggcggaa acgctgaaag tgactgacga ggcgcagcgt acagctctgt acactaaagc
     1681 agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg tgaatgcgcg
     1741 tttggtgaaa ccgtgggttg gtggctatac cggcaaagat ccgctggata atacctacac
     1801 ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggtgt gtcctgctcc
     1861 acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg gcaatgttaa
     1921 aatttattct acgtcgctgt ctggaagcga ttccgacgct atttattctt attactattt
     1981 cgttctttat gatgcgcctc gctccgggca gtccttttac cggcgaacgt actttaccac
     2041 cggaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc atgacacagt
     2101 atttcagcta cctgaaacaa ctggcgcatg gcgatttcgg tccatcgttt aaatataaag
     2161 attattcggt caatgacctg gttgcatcca gttttcccgt ttctgccaaa ctgggagccg
     2221 cagcattttt tcttgcggta atactgggtg ttagtgctgg cgttattgcc gcattaaagc
     2281 aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt gttatcccca
     2341 gttttgtggt tgcgccatta ttagtcatga tatttgcgat cattttgcat tggctgccgg
     2401 gcggtggctg gaatggtgga gcgcttaaat tcatgatatt gccaatggtg gcgttatcac
     2461 tcgcctatat cgccagtatt gcgcgtatta cccgtggctc tatgattgag gttttacact
     2521 ccaactttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc attttacgcc
     2581 acgcattaaa acctgctctg ttacccgtgc tctcctatat ggggcccgca tttgtcggca
     2641 ttattaccgg ttcgatggtc atcgaaacca tttatggttt accggggatt gggcaattgt
     2701 tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagtctgacc attctggttg
     2761 gcgcgttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt atcgacccga
     2821 aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca gcgagacgct
     2881 ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg acgcacgtcg
     2941 acgttttatg cataaccgtg cggcggttgc cagtctggta gtgctggtgc tgatcgcgtt
     3001 atttgtaatt ctggcaccga tgctttcgca gtttgcctat gacgatactg actgggcgat
     3061 gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact catccggtcg
     3121 cgacctgctt gtgcgcgttg cgattggcgg gcgtatctca ctcatggtcg gtgttgctgc
     3181 ggcactggtg gcagtggtcg tggggacact ttatggttcg ctttccggtt atctgggcgg
     3241 taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc cattcatgtt
     3301 cttcgtcatt ttgctggtga cctttttcgg tcaaaacatc ctgctgattt tcgtggcgat
     3361 tggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc tgagtctgaa
     3421 gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacgccgg gcattgttat
     3481 tcgccacatt gtgccgaacg tactcggtgt ggtggtggtc tatgcgtcgc ttctggtgcc
     3541 cagcatgatc ctctttgaat ctttccttag cttcctggga ttaggtacgc aagagccgtt
     3601 aagcagctgg ggtgcattgc tgagtgatgg cgcgaactcg atggaagtct ctccatggtt
     3661 actgttgttc cctgcgggat tcctcgtggt aacgctgttt tgtttcaact ttatcggcga
     3721 tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat gagcgtaatt
     3781 gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa cgtgaaagat
     3841 ttgcgtgtca cctttagtac cccggatggc gacgtcacgg cggtcaatga tttgaatttt
     3901 tcgctacgtg ccggagaaac gctgggcatc gtaggtgagt ctggttcggg caaatcgcaa
     3961 accgcatttg cgttgatggg cctgttggct gccaacggac gtattggcgg atcggcaacc
     4021 ttcaatgggc gtgaaatcct caatttgcca gagcgtgaac tcaataaact gcgagctgaa
     4081 caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat gcgcgtcggt
     4141 gagcagttga tggaagtgct gatgctgcat aagaacatga gcaaagctga ggcgtttgaa
     4201 gaatcggtgc ggatgctcga tgcggtaaaa atgccggaag cgcgtaaacg catgaaaatg
     4261 tacccgcacg aattttctgg cggcatgcgt cagcgagtca tgattgcgat ggcattgcta
     4321 tgtcgaccta agctgctgat tgcggatgaa cccactactg cgctggacgt caccgtacag
     4381 gcgcagatca tgacgctatt gaatgaactg aagcgggaat ttaataccgc catcattatg
     4441 attacccacg atcttggcgt ggtggcgggg atctgcgaca aagtgctggt aatgtacgcc
     4501 gggcgcacga tggaatatgg caacgcgcgc gatgtctttt atcaacccgt acatccttat
     4561 tctatcggtt tgctcaacgc ggtgccgcgt ctcgatgcgg aaggtgaaac aatgttgact
     4621 attccgggta atccgccaaa cctgctgcga ttaccgaaag gttgcccgtt ccagccacgt
     4681 tgtccgcatg cgatggaaat ttgtagtagc gcaccgccgc tggaagagtt tacgcctggc
     4741 cgtctgcgtg cttgctttaa accggtggag gaactgttat gaatgctgta accgaaggaa
     4801 gaaaagtcct cctcgaaatc gccgatctga aagtgcactt tgaaatcaaa gatggcaaac
     4861 agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtaact cttcgcctgt
     4921 atgaagggga aacattaggt gtggtagggg aatcgggatg cggtaagtcc acctttgctc
     4981 gcgccatcat tggtttggtc aaggcgaccg acggtcatgt tgcctggtta ggtaaagagt
     5041 tgctgggcat gaagcccgat gaatggcgtg ccgtacgcag tgatattcag atgattttcc
     5101 aggatccgtt ggcatcgctg aacccgcgta tgaccatcgg cgagatcatc gctgaaccac
     5161 tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg aaggcgatga
     5221 tgctgaaagt cggtttgttg cctaacctga ttaaccgcta tccgcatgag ttctccggtg
     5281 ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag ctgattatct
     5341 gtgatgagcc ggtgtcggcg ctggacgtgt caattcaggc gcaggtggtc aacctgctcc
     5401 agcagttgca acgtgagatg ggattgtcat taatttttat cgctcacgac ctggccgtgg
     5461 taaaacacat ttccgatcgt gtgctggtga tgtatctcgg ccatgcggta gaactgggga
     5521 cctatgatga ggtctaccac aatccgctac atccttacac cagggcattg atgtcggcag
     5581 tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg gaaggggaat
     5641 taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgt ccgattgccg
     5701 gtccggagtg cgccaaaaca cgtcctgtgc tggaagggag tttcagacac gccgtttctt
     5761 gcctgaaagt cgatccgctt taaaagcaat aagggctgac agttgtcagc ccttattcac
     5821 gccaaaagcg attatttatt cccgccagat gatatggcaa agtttgtgat ctttttcgcg
     5881 gcataacaga atgcgcgcga aaacatcgtt gatttcacca tcttcactgt ccgccagacc
     5941 aatcaccact tcggcaaaaa agtccgggtt caaatcgaaa tcaacatgtt cctgccagtc
     6001 ttccgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat tgaacagcag
     6061 aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt aggcctgctc
     6121 aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca ctacctgttt
     6181 aacctctgtt ggcgacgttt tacagcaacg ggctgaagaa gtaaaacagt cgctcggcga
     6241 cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacga gaacgcgaaa
     6301 tataatcgtc ctgaacggcg gcgaggtctg caccaaaacc tttatcgtcg attgccaggg
     6361 taatctcgaa atttagccac agactacgca tatcaaggtt aacggtgcca accagactta
     6421 gttcaccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac tgataaattt
     6481 taaccccagc agccagcagt tctgtaaaga atgcgcgact ggcccagccg accagcatcg
     6541 agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc gccgtgcaaa
     6601 tcgcatgaag taaatcatcg cttggcacaa aatagggcgt tgtcatgatc aaatattcac
     6661 gagccgaata agcagcagtc aataatgcct ggtgaatgag atcttccgga aagccggggc
     6721 cagaagcaat tgtgtgaata gtgtgaccgc tggcctgctc aaacggcata atattgacat
     6781 ctggtggcgg cggcagaata cgtttcccgg tttcaatctc ccagtcgcag gaataaataa
     6841 tccccatcgc ggtggcgatg gggccttcca tacgcgccat cagatcaatc cattgcccta
     6901 cgcccgcatc ttgtttgaag tagcgagggt cgaccatatt catgctgccg gtgtacgcga
     6961 tgtaattatc aatcatgatc atcttgcgat gttggcgcag gtccatacgg cgtaaaaaca
     7021 cacgcatcag attgaccttt aaggcttcga caacttcaat accagcatta cgcattagct
     7081 cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc aatcggcaat
     7141 gaataccgcg ccgtgcagcg gccattaatg attcagccac ctggtccgcc atgccgccgg
     7201 gctgccagat ataaaacacc atctcaatat tatggcgcgc gagctggatg tcgcggatta
     7261 acgcctgcat cacatcatct gactcggtca tcagttgtag ctgattccct ttgaccccag
     7321 cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg ctattttctt
     7381 cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg gaaggccaca
     7441 tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg gcaagatagg
     7501 caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc gcggagggaa
     7561 ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac cagtatccca
     7621 gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa tccttttgaa
     7681 agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac gcgggaaaag
     7741 cactggtcag cgtcaggggc gggggtataa tgactattct gttgttgcat agagtagtta
     7801 acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag gctagccgac
     7861 gtaaatcgcg ttggcttgag gggcagtcgc gccgaaatat gcgtatccac agcatcagga
     7921 agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat atctaaatgt
     7981 actgggcacc attgcggtgc ccaataagtt tactcttccg tctttaaact ctatttttgc
     8041 gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa aatgccatga
     8101 ccggagttca acaacattga aacgagcata tcattattaa tctcttcacc atttaaaact
     8161 cgcgccagaa tttcgctgcc aaacagttgt ggtgagagaa tgatatccgg atgtaccatc
     8221 ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac atcactgctc
     8281 atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact cagcgccaga
     8341 atggcccggc aacgatcgat tcccgctttc tttaacactg aactgtcatt actgtcaccg
     8401 gggataacat cggcgttatc gcctaagcgt tgctcaagtt gcttgatatc atcttcaggc
     8461 aagttgctga taaccgttac gttttgtccg cgttgattca gttgcagaat cgtattgatg
     8521 gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt atgattgttt
     8581 ccttttacaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga ggtcatagat
     8641 gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg tgctgattca
     8701 gaaacaggga caatatcgcc atagccgacg gttgacatgg tttctatcga aaaatagaac
     8761 gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag cgcaccgtaa
     8821 gtggagtaaa acagtaacgt cgtgaaacta ataaatgcga aaattgtccc ggctgctgcg
     8881 ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa aatgcaaaat
     8941 ccaatactaa atttcagcca gggataaaaa tgcagagtgt aaattagcgc tatcaacaac
     9001 aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat ggagttcagg
     9061 acaagaaata cgccgagcat aaatagtggg gcgtttgcca gcaaggagag atccagctct
     9121 gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata gattgtttta
     9181 aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg tagggtcacc
     9241 cataactttg ttgctgtttg tttgaatgta gtccagtgac tcactgatac ctctgtaacc
     9301 acaattttgt ttaattatct caacgtgcac attttctgaa catacatgca gcgcgtgaat
     9361 gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag tgagcaactc
     9421 tgcgtcagga aagggcattt tcctgaatat gttcacatca tataaagaaa tacagataaa
     9481 gttattatct gcttgtggtg gtgaatgcac tgaccgacct ataaggaaag gccaaacaag
     9541 aacacggttg caaaaaccgt gcccttaaat attgaatctc tattcagaac actttcttaa
     9601 atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca gcatcggccc
     9661 aggcctgagc tgcttccagc gactcaaatt cagcaataac ggttgagcca gtaaatcccg
     9721 cagcccctgg atcgttactg tctaccgctg gcattggacc ggcggtcaac aaacgacctt
     9781 catcatgcag taactgtaaa cgtgctaaat gtgccggacg aacggaaagg cgtttttcga
     9841 ggctatcagc tttgtcttga gcgtaaataa catacaacac aggcacaact ccttgttcgg
     9901 gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt
     9961 tgttaagacc atgcataaag taaggtcaat tacgccaaaa atgacatttt cactgatcct
    10021 gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgggg cctggttttt
    10081 ctactgaaat gattatgact tcaatgaccc ttgatttacc tcgccgcttc ccctggccga
    10141 cgttactttc ggtctgcatt catggtgctg ttgtggcggg tttgctctat acctcggtac
    10201 atcaggttat tgaactacct gcgcctgcgc agccgatttc tgtcacgatg gttgcgccag
    10261 cggatctcga acctccacaa gccgttcagc caccaccgga gccggtggta gagccagaac
    10321 cggaacctga gccgatccct gaaccgccaa aagaagctcc ggtggtcatt gaaaagccga
    10381 agccgaaacc taagccaaaa ccgaagccgg tgaaaaaggt ggagcagcca aaacgcgatg
    10441 tcaaacccgt agagtcgcgt ccggcatcac cgtttgaaaa tacggcaccc gcgcgcccga
    10501 catcaagtac ggcaacggct gcaaccagca agccggttac cagtgtggct tcaggaccac
    10561 gcgcattaag ccgtaatcag ccgcagtatc cggcacgagc acaggcattg cgcattgaag
    10621 ggcaggttaa agttaaattt gatgtcacgc cagatggtcg cgtggataac gtacaaatcc
    10681 tctcagccaa gcctgcaaac atgtttgagc gtgaggtgaa aaatgcgatg cgcagatggc
    10741 gttatgagcc gggtaagcca ggcagtggga ttgtggtgaa tatcctgttt aaaattaacg
    10801 gcactaccga aattcagtaa gcataaagtc aaaagcctcc gaccggaggc ttttgattat
    10861 tactcaacgg gtaaggcgcg aggttttcct tcgggatcaa ccgcgacata cttaaataat
    10921 gcttctgttg ctttatagcg ttgcccgatt ggttcagacg ccactttttt cacccacact
    10981 tcaatattaa tgctgactga tgtcgtccct ttctggacac agcgtgcata gcagcacacc
    11041 acatcgccga ccgcaaccgg ccgtaagaaa gtcattcctt caacccgcac ggtcactaca
    11101 cgaccatggg caatttcttt cgccagaata gcgccgccaa tatccatttg tgacattaac
    11161 caaccaccaa agatgtcacc attggcattg gtatcggcgg gcatggctaa agtacgtaaa
    11221 acaagatcgc cctgagggac gttatgtgtt gtagacatgg taaaaccgac ttaaaaggaa
    11281 aatcacaggc gcgatgctac tatgattttc ctgaggagaa cagaggagat acagcgccag
    11341 cccctgaagg actggcagtc tggttaggat ttatcttcct gcggcatgtg gcggtagata
    11401 tagataccgc ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa gactttaaag
    11461 ttgacccaaa tattttgcgg cagccagaat gcgatgtaga tgtttgccag accgcaaagg
    11521 ataaagaaaa cagcccaggc cagattcagc ttcgaccata ccgattgcgg cagggtgagt
    11581 tctttaccca gcatccgctg aattagcggc tttttcatca cccattggct gactaacagg
    11641 gcacccgcaa acagggcata aatgacagta accttccatt taataaactc atcattgtgg
    11701 aagaacagcg ttaagccacc gaagacgacc accagaacaa aagtgatcag ggccatcttc
    11761 tcaaccttac gaaagcgaac ccagctatat ataagcacaa tcgccgtggc gacgatgagc
    11821 gccgcagtag ccgcatagat gtcataaatc ttgtaaaacg cgaaaaagac aaccagcggt
    11881 aaaaaatcaa gaaactgctt cattttacga ttccgtaatc aaatgcttaa ggttattggc
    11941 gaatcaacat atacaggcgg aacagataga tgagcaatac ggctgaaatc aggttgctca
    12001 aggtgttcgc cagtacggca ccaatttccg gggttaatgc ggcaaaagaa gaggcaaaaa
    12061 gcagcaacag tgtttttgcc agcaaccagc tcagtactgc gggtgccacc agacgcatat
    12121 tcgcccatgt cagccgcata ctgctacgca tcgaggcaaa aacgcccatt ttgtcctgta
    12181 ccagcatcac tggagccaga gccagtaaaa tggccatgat aatccccgga acgaccacca
    12241 gcatgatgcc aatctgtact aaaagggtag tcagaaaaat cagaataaat aactttggca
    12301 atatcggcgc actggcacca atagctcgca gcgcactgac tctctgaccc gcagacacca
    12361 gctggataat taatattacg cccccggcga gaatggcgtt accgattaat cctgaaaaag
    12421 tggacgccgc tgaagcctgc agcaaaattt gttgctgttc cggtgacata ttctgaacca
    12481 ggtcgaacaa cccactactg ccgctaacgg gtacgccgtc attgagctgc gcaagctgtg
    12541 catcactggg tgagaaaaca tgccctaaca ccactgtgat aaacgcacat agcaacgata
    12601 ccaacagaat ggtcataaat tgattacgga agaaatttcc ggtgtcacgg tatacggact
    12661 gcgccgtgat agacatgcac tctccttgag ttttatgcag gtgttaatta gcgggcaatt
    12721 gtaccctggt taagccccgg gtggcagcat caggtcttgt atggaaaagc atatctttgt
    12781 aaagcggagg taattgctgg ccttcaggcg tgggtaaacc gctgcaagtg gcgagcgagt
    12841 taatatttgc gtagcgaaaa tataaaaatt gatttaaatc acattaacct ggattttcaa
    12901 tgcaacttct aaattaatcc agatcaataa agggtgaatt accatatgta atgtgatctg
    12961 tgtaggatca tttgttaccc caatgtgggt atattcgtca cgtttttata accataacga
    13021 cggagcggat atgaaaaagt taacagtggc ggctttggca gtagcaactc ttctctctgg
    13081 cagtgccttt gcgcatgaag ccggcgaatt ttttatgcgt gcaggttctg caaccgtacg
    13141 tccaacagaa ggtgctggtg gtacgttagg aagtctgggt ggattcagcg tgaccaataa
    13201 cactcaactg ggcctgacgt ttacttatat ggcgaccgac aacattggtg tggaattact
    13261 ggcagcaacg ccgtttcgtc ataaagttgg cacagccact accggggata ttgcaactgt
    13321 ccaccattta ccgccaacac tgatggcaca atggtatttt ggcgattcag gcagtaaatt
    13381 ccgtccttac attggtgcgg gtattaacta caccaccttt tttaataatg acttcaatga
    13441 caccggtgaa ggtcttggtt tatctgacct tagtctgaag gattcatggg gcgtggcggg
    13501 gcaagtaggt ctggattatc tcatcaaccg tgactggttg ctgaatatgt cggtctggta
    13561 catggatatc gataccgaag tgaaatataa atccaacggc gcagtgctgc ctgcagggcg
    13621 ttattctgac aacgtacgcc ttgacccgtg ggtatttatg ttctctgcgg gttaccgctt
    13681 ctaatttttt accatcaata aaccgccaga tgttttctgg cggtttgtct caaattaacg
    13741 tcgtgtagcc gctttcatcg gttgtacaaa agctttcagt gccgccagca ttttctctgg
    13801 ctcattaata tgttgctcga tgattttaac gatggccgaa ccagaaatcg cgcccgcagc
    13861 tcctgcatca atcgctgctt ttacctgatc cggggcggaa ataccaaatc cctgcaatgg
    13921 aggcgcagcg ttgtactctt tcagcttcgc aaccagatga ttgaggggta acgcggcgcg
    13981 gttttctgcg ccggtcacgc ccgctcgcga cagcaaatag gtgtaaccac gaccgtaaga
    14041 ggctatctgg cgcagcaaat catcgtcggc attaggcggg caaataaaga taggtgcgac
    14101 attatgacgc aacgcggcct ggcggaaggg cgcggactct tccacgggca catcggcaac
    14161 cagcaccgaa tcgacgccga ctttctcgca ctcggcataa aactcatcaa tgcctttgtt
    14221 aaacaccagg ttggcataca tcaaaaggcc gatgggaatg gtcgggtgct tctggcgaat
    14281 gagtgccagc acctcaaagc actgcgccgg ggttactccc gccgcaaaag cacgcagtgt
    14341 ggcgttttga atcgtcgggc catccgccag tgggtcggag aaggggatgc ctaattccag
    14401 cgcgtcagca ccggcttcaa ttagcgtatc gataattttc aacgactgct caatgcccgg
    14461 atcaccgagg gtgacgaaag gaacgaatgc gccttctttg cgctccttca actgggcaaa
    14521 cagagattcg tagcgttcca tcagatttcc cctcgtgctt tcaaaatatc gtgaacggtg
    14581 aagatgtctt tatcgccgcg accggaaagg ttaaccacca gtagctgctc tttttccgga
    14641 ttttcgcgca tcattttcag cgcatgggcc agggcgtggg aggattccag cgccgggatg
    14701 atcccttcat gcaggcaaag cgttttaaag gcttccaggg cttcatcgtc ggtaatagac
    14761 acgtaatcag cgcgtccagt gctgttgaga tacgcatgtt gcgggccgac ggacgggaaa
    14821 tccagcccgg cagaaatgga gtaagactct tcaatctgcc cgtcttcggt ttgcatcatc
    14881 ggcgctttca taccgaaata gatgcccacg cgaccatgtt ttaacggtgc gccgtgctcg
    14941 ccagtttcga taccgtggcc gccaggctcc acaccaatca ggccgacgtc ggtttcgttg
    15001 atgaaatctg caaacatgcc gatggcattc gaaccgccgc caacacaggc gataaccgca
    15061 tccggcaggc gaccttctct ttccagaatc tgcgctttcg tttcttcgcc aatcatccgc
    15121 tgaaactcac gcacaatggt cgggtaagga tgcgggccag ctgcggtacc cagcatatag
    15181 tgcgcggttt cataactgcc ggaccagtcg cgtagcgcct cattacaggc atctttcagc
    15241 gtcgcggaac cgctatgtac cgggatcact tccgcaccca ttaagcgcat acgaaaaacg
    15301 ttgggcgact ggcgttcaac gtctttggca cccatataaa tacggcattt caggccgagc
    15361 agggcgctgg caagggccga cgccacgcca tgctgaccgg caccggtttc ggcaataatt
    15421 tcagttttac ccatccgctt cgccagtaaa gcctgaccga gcacctggtt agttttatgc
    15481 gcgccgccgt gcagcaaatc ttcgcgcttc agatacagcg tggtgttcgt cccggctgta
    15541 atgttctggc atttggtcag cgcggttgga cgcccggcat agtttttcag caggtcgttg
    15601 aactgagcct gaaattcagg atctttttgc gcgctgacaa aagcttcttc cagctggcgc
    15661 agagcaggca tcaggatttg tggcacgtac atgccgccaa actcaccaaa ataggggtta
    15721 agtaatgttg tcattgttcc tttccttaat atgcgcgcag cgtctggaaa accgaggcca
    15781 aaagacgtgc gtctttgata cccggttgcg actctacagc agaattaaaa tcaagcccgg
    15841 cgcagccggt ttgtgccgct tccacgcagt tatctgcgcc taagcccccc gccagcagaa
    15901 cgttgccaag cgattgacca tttaatagtg accagtcgaa acgttgtccg ctcccgccct
    15961 gaccgttgtc gaatacatat ttatcgatgt gctgaaaatc gcgcgcggga agagtttcac
    16021 cgacacttaa agccttccag atggcgacgt gtgctggcag agcctcacgc agattgtcga
    16081 tatacagctg atcttcatta ccatgcagtt gcactgccgc cagcgataac accttagctt
    16141 tgtccgccac atcggcaata tcgtgattgc ggaacacgcc aacatactgc aacggtgccg
    16201 cagccatcac ttcctgcgcc tgttcaacgt tgacgcaacg cggtgatgtc gcaacaaaaa
    16261 tcaacccacc gtaaatcgcg cccgcgtcat aagctgcttt agcatcttgc ccacgtgtca
    16321 ggccacatac tttattctca cccagcaaca cccgacgcac ggcggcgttc aaatcgtcat
    16381 gggccatcaa cgccgaacca atcagaaagc cgttagcgaa gtggcttaac tcgcgcacct
    16441 gagcgtaagt attgatgccg gattcgctga ttaccgtcac gttgtgcccc agtttcggtg
    16501 caagctcgcg ggtacggttg agatcaatcg acaaatcgcg cagatcgcgg ttgttgatgc
    16561 caacgacctt tgcccccaat gcaatggcgc gctccagttc ctcttcatta ctgacttcgg
    16621 tcagcacacc catctccaga ctgtgggcga cggctgcaag ctggcgatat tgttcgtcat
    16681 ccagtactga aagcattaat aagcaggcat cggcctggta atagcgcgcc agatagatct
    16741 ggtaaggatc gataatgaag tctttacata aaatcggctg cggggcgatt tggctgacga
    16801 tggggaggaa atcaaagctc ccctgaaaat atttctcatc agtcagcacc gaaattgccg
    16861 aagcgtaatg tttataaatg gcggcaatgc gtgccggatc gaaatcatca cggatcacgc
    16921 cttttgacgg cgacgctttt ttacactcca gaataaacgc cgtgcgtgcg ccctgaagtg
    16981 catcataaaa atgtcgcgtg ctcggctgaa cctcattctg aaaactggcc agcggttgct
    17041 gctctttgcg ggtttctacc caaatcgcct tgtctgcgac gattttcgct aaaacggttt
    17101 gcatcattta ccctcgtgcc gccagtgcgg tgactctgtc gtaagcggaa ccactgcgca
    17161 gtacctcaag aacggtttgc gcattggctt gcagatcttc atggccatgc aggcgcatta
    17221 acatggcgac attcgccgcg acggctgctt catgggcggc gtcgccttta ccttgtaaca
    17281 agcgtgttaa aatgtcacga ttttcttccg gtgttccgcc tgccagttgc tcctggtggt
    17341 agggtgtcag gccaaagtct tctgcggtga gctgatagct tttaatttcg ccgtcatgca
    17401 gttcggcaac gattgtcggc gcgtgtaatg aaacttcatc catcccgccg ctgtgcacca
    17461 ccgccgcgcg ttgatacccc agcacgcgca aggtttcggc aatcggcagc accagttccg
    17521 gactataaac accaattaac gccagcggcg gatgcgccgg gttaatcaat ggccccagca
    17581 cattgaacag tgtgcgggtt tttagttgct ggcgaaccgg cattgcatgg cggaatccgg
    17641 tgtgatattt cggtgcaaag aggaaacaga cgcctaactc atccagcgcc tggcgcgatt
    17701 tatcggcgtt catatcaaga ttaataccga acgccgccag cagatccgac gagccggatt
    17761 tactggagac gctacggttg ccgtgtttcg ccactttcag cccgcaggcc gcggcgacaa
    17821 acgcactggc ggtagaaata ttgatgctat tactgccgtc accgccggtg ccgacgatat
    17881 cggcaaacag ataatccggg cgcgggaacg gcgcggcgtt ttccagtagc gcggttgctg
    17941 ctccggcgat ttcattcggg tgctcgccgc gaattttcat gctcaccagc gctgccgcca
    18001 gttgttccgg tttcagttcg ccacgcacca ccgctgaaaa tagctggtgg ctttcctgct
    18061 ggctaagggt ctgcgcctga tacagttttt ccagaatcgg ttgcagcgtg ttggttggct
    18121 ctagtttctg ctgcgcccag gccagcgttt gttccagcag gcgagcgccc tgggtagtaa
    18181 gaatggattc cggatggaac tggaatccac aaacgcgatc cgcatcatgg cgcaccgcca
    18241 tcaccatgcc attaaaatgg gcgttgatgg ttaaaccggc cggaatatta ctgccaacca
    18301 gcgagtgata acgcgccact ggcagcgggt ttgttaatcc ggcaaacatc gcctgaccgt
    18361 catgttcaat gctcgacgct ttaccgtgaa gaatttcgcc cgcctgaccg acatagcccc
    18421 cgtaagcttc gacaatcgcc tgatgaccga ggcaaatgcc aataattggc agcttgccgc
    18481 gcaggcgggt gaggagttcc ggcatacaac cggcttcgct cggcacaccg gggccaggag
    18541 agagcatcag caccggattg ctcatcgtcg ccaggcgttc aattaaggtc tgcgccggaa
    18601 tatggttgcg gtaaatcacc acgttatgac cattgctgcg caactgatct gccaggttgt
    18661 acgtaaaaga gtcgatatta tcgagcagca gaatgtcagc cattagaacg tctcctgtgc
    18721 atgatgcgcg gtggcaatag cgcgcagtac agcgcgggct ttattacggg tttcgtctgc
    18781 ttccgactgc ggaacagaat caaggactac gccagcaccg gcttgcacgg tggcgatacc
    18841 gttttccacc agcgccgagc ggatcacaat gcaggtgtcg agatcgccat gcgcggtaaa
    18901 ataacctacc gcgccgccgt agctgccgcg tcgacgacct tctgcttcgg caattaactg
    18961 catagcgcgt actttcggtg caccgcttaa cgtccccata ttcatacagg cgcggtaagc
    19021 gtgcagggcg tcgagatcgt ggcgcagctc accaacaacg cgggagacca ggtgcatcac
    19081 gtaagagtaa cggtcaactt tggtgagatc ggcgacgtag cggctgccgg gtgtgcaaat
    19141 gcgagccagg tcattacggg cgagatccac cagcatcaga tgttcagaaa gctctttatg
    19201 gtcggtacgc atttcgagtt cgatgcggct gtcgaggtct ctgtccagtg aaccatcggc
    19261 acgacgaccg cgtggacgtg ttccggcaat cgggtaaatc tcaatctggc ggctggtggc
    19321 gtcatacttg agcgaacttt ccggcgacgc gccaaacagg gtgaaatcat tatcctgcat
    19381 aaaaaacatg tacgggctgg gattactctt tttcagcacg taataggccg ccagcggtga
    19441 cgggcagggc agagagaaac ggcgagatgg caccacctgg aaaatttctc cggcgcgaat
    19501 cgctttttgc aacaaacgca ctacgccacc gaactcttca tcgctctggt tacattcaca
    19561 acgcatatgc ggcacggaaa ccaccggcag cggcggcgcg gcttcggtta gctgttggcg
    19621 aagttcagca aggcgagcag tgagacgttg tttttcatct tcattcgaag caaacaggct
    19681 ggcctgaata cgggtgcttt ttttctgatg gtcaatcacc atcagcgttt cagcgagata
    19741 aaaacagaaa tcagggcagc tattttccgc tgccagttgt ggtaaatctt caaatcccgc
    19801 cacaaggtca taagagaaca ggccgccgaa gaacattgct tctcgttctt ccttcggtac
    19861 attcaacaga ttctgcaata aacggaaagc gtcaaaaacc gaaagggagc ataagcgggc
    19921 atcttcatcc agcagtggac tgacaggggg gaagcgcagc acgcggcagt ttggtgattg
    19981 ttcattttcc acacccgcag gcaaggcgtt atccagtagt gtcaacaggg cttctccatt
    20041 gccggaaagc gcctggattg tgacagtgtc acttaatgct gtaatgcgca gcgcactgtc
    20101 taccagcagc aggcttttta aatcatcttt gctgtcgata tctgcggatt ccagcagcag
    20161 cgttgccgga cgatccccac acaactggtg aaaaagcgca gtcgggttgt cgcgataagc
    20221 gcctttgcag gttagcagtt cgagagtcgg tttttgtgtt tgcattgtta ttctctaatt
    20281 ttgttcaaaa aaaagcccgc tcattaggcg ggctgggtat ctgattgctt tacgcatggt
    20341 gaatacactg cccgtttcag gaagtgcgcc accaaccttt cagtacgaaa attgctttca
    20401 ttgtcgatac cctttttacg tgaacttgcg tactagttaa ctagttcgat gatcaattgt
    20461 caacagctca tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat
    20521 ccagaacggg agtgcgcctt gagcgacacg aattacgcag tgatttacga cctgcacagc
    20581 cataccacag cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag
    20641 atgcgcgtcg gcaccctggc gatcaccgac catgacacca cagctgcaat tgcaccagca
    20701 agagaggaaa tttcacgttc gggactggcg ctgaatctta ttcctggcgt ggaaatttcc
    20761 acggtctggg aaaatcatga aattcatatt gtcgggctga atattgatat tactcatccg
    20821 ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca atcagcgggc gcagctgatt
    20881 gccgaaagac ttgaaaaagc gcaaatacct ggggcactgg aaggcgcgca acgactggcg
    20941 cagggcggtg cggtgacgcg cggtcatttt gcgcgtttcc ttgtggagtg cggaaaagcc
    21001 agttcaatgg cggatgtctt taaaaagtat ctggcgcgcg ggaaaaccgg gtacgttccg
    21061 ccacagtggt gtacaataga gcaatctatt gatgtcattc atcattctgg cggtaaggcg
    21121 gtgctagctc atcctgggcg gtacaatctt agtgctaaat ggctgaaaag attggttgcg
    21181 cattttgccg aacaccacgg cgacgcgatg gaagtcgcgc agtgccagca atcgcccaat
    21241 gaacgcaccc aactggcgac acttgcgcgt cagcatcatt tatgggcatc acaaggatct
    21301 gattttcatc agccatgccc gtggatcgaa ctgggtcgta aactctggct gcccgcaggc
    21361 gttgaaggcg tctggcagct atgggaacag ccgcagaaca ccacagagag ggaattatga
    21421 gccagttttt ttatattcat cctgataacc cgcagcaacg tctgatcaac caggcggtgg
    21481 agatcgtgcg taaaggcggg gtgattgttt atccaactga ttccggctat gcgctcggct
    21541 gtaaaattga agataaaaac gcgatggagc gtatttgtcg tattcgccat ctgccggacg
    21601 gtcacaactt taccctgatg tgtcgcgatc tttctgagct ctcgacctat tcattcgtgg
    21661 ataacgttgc gtttcgtttg atgaaaaaca acacgccggg caactacacc ttcatcctga
    21721 aagggacgaa ggaggtgcca cgccgtctgt tgcaggaaaa acgcaaaacc atcggtatgc
    21781 gtgtgccttc taacccgatt gcgcaggcgt tgcttgaagc attgggcgaa ccgatgcttt
    21841 ccacttcgct aatgctgcca ggcagcgaat ttaccgaatc cgatccggaa gaaattaaag
    21901 accgtctgga aaaacaggta gatttgatta ttcacggcgg ttatctcggg cagaaaccaa
    21961 caacggttat tgatctcacc gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg
    22021 tgaagccttt cttataacaa attgcgttcc acggatgggt gaatatggca gggatatatc
    22081 gctgcatttt gctattgata gttgggcttt ctttctcttc gttaagttat gcgaaaaaca
    22141 cggagatgcc ttcttatgaa gaagggatct cgctctttga tgttgaagcc actctgcaac
    22201 cggatggggt gctcgacatc aaagagaaca ttcattttca ggcgcgaaat cagcagatta
    22261 agcacggatt ttatcgtgat ttaccacgac tatggatgct gcctaatggg gacgccgcac
    22321 tgctgaacta tcatattgtt ggcgtcaccc gtgatggtat tcctgaaccc tggcatcttg
    22381 actggcatat cgggttaatg agtattgtcg tgggcgataa acaacgtttc ttgcctcaag
    22441 gcgactatca ttatcaaatt cattatcagg ttaaaaatgc tttcctgcgt gagggggatt
    22501 ctgatctgct aatctggaac gtgactggta ataactggcc gtttgaaatc tataagaccc
    22561 gattttctct ccagttgcct gatattgcgg gtaatccatt tagcgaaatt gatctcttta
    22621 ctggagaaga gggcgacaca tatcgaaatg gccgcatcct tgaggacgga agaattgaat
    22681 cccgcgatcc gttttatcgt gaagacttca cgatcctcta ccgctggcct cacgctttac
    22741 ttggtaatgc cccggcacca caaacgacga atattttcag ccatcttctt ttaccctcta
    22801 cgtcatcgtt gttaatttgg tttccgtgtc tgttcctggc gtgtggatgg ttatatctct
    22861 ggaagcgcag gccgcaattt acgccggtag atgtgattga aaccgatgtc tttccgccag
    22921 attacacgcc cggcatgtta cgtctcgatg cgaaactggt ttacgacgat aaaggttttt
    22981 gtgccgatat cgtaaatctg attgtgaaag gcaaaattca tctgaaagat cagtatgaca
    23041 agaaccagca aatcctgatt tgtgttaatg aaggcgcgac cagaaataat gaggtattac
    23101 tgcccgcaga gcagttatta ctggaagagt tatttcgtaa aggcgataag gtcgttctta
    23161 cggggagacg caacagagtc ttacgcaggg catttttacg gatgcagaaa ttttatctgc
    23221 cgcgtaaaaa gtcttcgttt taccggcctg atacgttttt gcaatggggc ggaatggcga
    23281 tattggcggt cattctctac ggtaacctga gtcccgtagg ttgggcagga atgagtctgg
    23341 ttggcgatat gtttattatg atctgctggc ttcttccttt tttattttgt tcccttgagc
    23401 ttttgtttgc ccgcgatgat gacaagcctt gtgttaatcg tgtaatcatc actttgtttt
    23461 taccgctgat ttgttcaggc gtggcctttt attctctcta tatcaatgtc ggagatgtat
    23521 tcttttactg gtatatgcca gcgggttatt ttagcgctgt tttcctgacc ggttatctca
    23581 ctggcatagg gtatattttt ctgccaaagt ttacccaaac tgggcagcaa cgttacgccc
    23641 acggtgaagc tatcgttaac tatcttgcgc gtaaagaggc ggcaacacac agtgggcggc
    23701 ggcggaaagg ggaaacacgg aaactggatt acgcgttgtt aggttgggct atatcggcaa
    23761 atttggggag ggaatgggcg ttacgcatcg ccccttcgct ttcttcggcg atttgcgctc
    23821 cagagattac ccgtaacggc gttttgttct cattacagat gcatctgagt ctgggggcca
    23881 ataccagttt gttggggcg
//
LOCUS       DAZKHK010000084        23795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKHK010000084 DAZKHK010000000
VERSION     DAZKHK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.084"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            380..1597
                     /gene="alaA"
                     /locus_tag="ACHOS5_003287"
     CDS             380..1597
                     /gene="alaA"
                     /locus_tag="ACHOS5_003287"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP9778056.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1681..2280
                     /gene="yfbR"
                     /locus_tag="ACHOS5_003288"
     CDS             1681..2280
                     /gene="yfbR"
                     /locus_tag="ACHOS5_003288"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9778057.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2339..4171)
                     /locus_tag="ACHOS5_003289"
     CDS             complement(2339..4171)
                     /locus_tag="ACHOS5_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9778058.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4258..4908)
                     /gene="hxpA"
                     /locus_tag="ACHOS5_003290"
     CDS             complement(4258..4908)
                     /gene="hxpA"
                     /locus_tag="ACHOS5_003290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP9778059.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4919..5413)
                     /locus_tag="ACHOS5_003291"
     CDS             complement(4919..5413)
                     /locus_tag="ACHOS5_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9778060.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5496..5951)
                     /gene="yfbV"
                     /locus_tag="ACHOS5_003292"
     CDS             complement(5496..5951)
                     /gene="yfbV"
                     /locus_tag="ACHOS5_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP9778061.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6289..7491
                     /gene="ackA"
                     /locus_tag="ACHOS5_003293"
     CDS             6289..7491
                     /gene="ackA"
                     /locus_tag="ACHOS5_003293"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP9778062.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7566..9710
                     /gene="pta"
                     /locus_tag="ACHOS5_003294"
     CDS             7566..9710
                     /gene="pta"
                     /locus_tag="ACHOS5_003294"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9778063.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9900..11420
                     /gene="yfcC"
                     /locus_tag="ACHOS5_003295"
     CDS             9900..11420
                     /gene="yfcC"
                     /locus_tag="ACHOS5_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9778064.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11453..11995)
                     /gene="yfcD"
                     /locus_tag="ACHOS5_003296"
     CDS             complement(11453..11995)
                     /gene="yfcD"
                     /locus_tag="ACHOS5_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP9778065.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12053..12604)
                     /gene="yfcE"
                     /locus_tag="ACHOS5_003297"
     CDS             complement(12053..12604)
                     /gene="yfcE"
                     /locus_tag="ACHOS5_003297"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP9778066.1"
                     /translation="MKLMFASDIHGSLPATERVLELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12660..13304)
                     /gene="yfcF"
                     /locus_tag="ACHOS5_003298"
     CDS             complement(12660..13304)
                     /gene="yfcF"
                     /locus_tag="ACHOS5_003298"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP9778067.1"
                     /translation="MSKTAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSSE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13440..14087
                     /gene="yfcG"
                     /locus_tag="ACHOS5_003299"
     CDS             13440..14087
                     /gene="yfcG"
                     /locus_tag="ACHOS5_003299"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP9778068.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            14144..14506
                     /gene="folX"
                     /locus_tag="ACHOS5_003300"
     CDS             14144..14506
                     /gene="folX"
                     /locus_tag="ACHOS5_003300"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP9778069.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14527..15420
                     /locus_tag="ACHOS5_003301"
     CDS             14527..15420
                     /locus_tag="ACHOS5_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP9778070.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQNDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHTLGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15468..16358)
                     /gene="rpnB"
                     /locus_tag="ACHOS5_003302"
     CDS             complement(15468..16358)
                     /gene="rpnB"
                     /locus_tag="ACHOS5_003302"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP9778071.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDARRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMMEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(16554..17327)
                     /gene="hisP"
                     /locus_tag="ACHOS5_003303"
     CDS             complement(16554..17327)
                     /gene="hisP"
                     /locus_tag="ACHOS5_003303"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP9778072.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17335..18051)
                     /locus_tag="ACHOS5_003304"
     CDS             complement(17335..18051)
                     /locus_tag="ACHOS5_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9778073.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18048..18734)
                     /gene="hisQ"
                     /locus_tag="ACHOS5_003305"
     CDS             complement(18048..18734)
                     /gene="hisQ"
                     /locus_tag="ACHOS5_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP9778074.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18824..19606)
                     /gene="hisJ"
                     /locus_tag="ACHOS5_003306"
     CDS             complement(18824..19606)
                     /gene="hisJ"
                     /locus_tag="ACHOS5_003306"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP9778075.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19827..20609)
                     /gene="argT"
                     /locus_tag="ACHOS5_003307"
     CDS             complement(19827..20609)
                     /gene="argT"
                     /locus_tag="ACHOS5_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP9778076.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDVIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALNELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20875..21444)
                     /gene="ubiX"
                     /locus_tag="ACHOS5_003308"
     CDS             complement(20875..21444)
                     /gene="ubiX"
                     /locus_tag="ACHOS5_003308"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP9778077.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(21539..23056)
                     /gene="purF"
                     /locus_tag="ACHOS5_003309"
     CDS             complement(21539..23056)
                     /gene="purF"
                     /locus_tag="ACHOS5_003309"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9778078.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQSYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23093..23581)
                     /gene="cvpA"
                     /locus_tag="ACHOS5_003310"
     CDS             complement(23093..23581)
                     /gene="cvpA"
                     /locus_tag="ACHOS5_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP9778079.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
BASE COUNT         6010 a         6291 c         5785 g         5709 t
ORIGIN      
        1 cttaacaata gtttcacgcc ttgcccccat tgtgacacct cggaaatcat cacgctgtta
       61 aactctccgt aatgcggaaa tatcatcatc gcgattcagt agtgaattct taaacgaggc
      121 attttcacac aattatctta cagataaaaa aaccacactt acaattaaga atcagaacaa
      181 ttcactacat aacattactt attaagcata tgcacctcat tattttgcca ttattaagtt
      241 attaacagca caatcgagcc ttcccctctg gcaaaatctt attctgcaga ccttcaaaac
      301 accgtcctgg gggagtacat tgttctaagc tgacttccac ggcagggagt ggcgataaca
      361 gcaaaaaagg tcaagattca tgtcccccat tgaaaaatcc agcaaattag agaatgtctg
      421 ttatgacatc cgtggtccgg tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa
      481 ggtactgaaa ctgaacatcg gcaaccctgc cccgttcggt tttgacgcgc cagatgaaat
      541 tctcgttgac gtgatacgca acctgcctac cgctcaaggg tattgcgatt ccaaaggtct
      601 ttactccgcg cgtaaagcca tcatgcagca ctaccaggct cgtggcatgc gtgatgttac
      661 cgtggaagat atttacatcg gcaatggtgt atcggagctt atcgttcagg caatgcaggc
      721 attgctgaac agcggggacg aaatgttggt tcctgcacct gattacccac tatggaccgc
      781 agcggtttcg ctctccagcg gtaaagcggt gcattatctt tgcgatgaat cctctgactg
      841 gttcccagac ctcgatgata ttcgcgctaa aattacgcct cgtacgcgtg ggatcgttat
      901 tatcaaccca aataacccaa ccggcgcggt atattccaaa gagcttttaa tggagattgt
      961 ggagattgca cgtcagcata atctcattat cttcgccgat gaaatttatg acaaaattct
     1021 ctacgacgac gctgagcatc actccattgc gccgctggca cctgacctgc tgaccattac
     1081 ctttaacgga ctgtcgaaaa cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt
     1141 gaacgggccg aaaaaacacg ccaaaggtta catcgaaggt ctggaaatgc tggcttcaat
     1201 gcgcctgtgt gctaacgttc ctgcgcaaca cgccattcag accgcgctgg gtggttatca
     1261 gagcatcagt gaatttatta cccctggagg tcgtctttat gagcagcgta accgcgcgtg
     1321 ggaattgatc aacgatattc cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat
     1381 gttcccgaaa atcgacgcca aacgctttaa cattcacgac gatcagaaaa tggtgctgga
     1441 tttcctgttg caggaaaaag tcctgttggt gcaagggacg gcattcaact ggccgtggcc
     1501 ggatcacttc cgcattgtca cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa
     1561 gttcgcgcgt ttcctttctg gttatcatca gctgtaatct taatttcact gccggagatt
     1621 gcatccggca gtgttatccc gccacaatga cctgatgatg tcatcatacg taaggtcact
     1681 atgaaacaga gccatttttt tgcccatctc tcccgcctga aactcattaa ccgctggccg
     1741 ctaatgcgca acgtgcggac ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc
     1801 gcccatgcgc tggcagctat caaaaatcgt aaatttggcg gtaatgtcaa cgccgaacgt
     1861 atcgctttac tggcgatgta ccacgatgct tcagaagtgc tcaccggaga tctccctact
     1921 ccggtgaaat acttcaattc gcaaatcgct caggaataca aggctattga aaaaatcgct
     1981 cagcaaaaac tggtcgatat ggttccggaa gagctgcagg atatctttgc gccgttaatt
     2041 gacgagcatg catatagcga tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt
     2101 gcatatctga aatgcctgga agaacttgcg gccggaaata atgaattctt gctggcaaaa
     2161 acgcgactgg aagcgacgct tgaagcgcgc cgcagccagg agatggacta cttcatggaa
     2221 gtatttgttc ccagcttcca tctttcgctc gatgagatta gccaggattc accgctgtaa
     2281 gcagccggag tctgcgtcgc atcaggcaat aagcaccgga tgcgacatca ggctcttgtc
     2341 aaaacggaaa cagcatcggg atcatcacca cacaaaccgc catcacgata atggtgaacg
     2401 gtacccccaa cttaacaaag tcactgaagc tgtaattccc cggacctaaa accagtgtgt
     2461 taacaggtga agatatcggt gtcataaagg cggcggatgc tgccatcgcc acgaccatcg
     2521 caaatggata aggcgacacc cccatcgttt tggcagcagc cagcgcaatc ggagccatca
     2581 acaccgccgt cgcggtatta gaaataaata gcccaataac cgccgacaag acaaacaaac
     2641 aacccagcat catatgtggc ccgtaaccgc cgccaatgtc catcagccct ttcaccgcca
     2701 gcgcgacacc tcccgttttc tgtaatgcca cagcaaatgg catcatccca acgatcaaaa
     2761 taatgctcgg ccagtgaatg gatttatagg cgctttcggc atcgatacag cggaatttcc
     2821 ccatcagcag gcaggcgatg atagcggcaa caggattagg aatttcatcg gtcagcatta
     2881 acgccaccat tagcaccaga cagaaaatcg catggggtgc ctggctgtgc gcgggcgatg
     2941 cttcactcac ctcttccggt aagttcagcg ctacgaagtc gcggccctgt ttggccagca
     3001 taccgatcag tttccagtta ccaacaacca gaatgatatc gcccagcagc agaggctcat
     3061 ccgccagcga accttccagc gccacaccat tgcgctttag ccccaccaca ttcaatccgt
     3121 agcgggtacg aaaaccaatt tcgcgcaccg atttaccaat aagttctgac tcaggaatta
     3181 atgaaatctc tgccatgccc acatcaaggg cctggtcaga aaaatactcg ccgcgcagta
     3241 ccatcggctc cagcaattgc tcactacaaa attgccggag atcgacatca gccgcagaca
     3301 tatcaataag caaaacgtca cgcgcgcgaa attcagaaac cccattaaca ttcacgataa
     3361 cgcgacgaaa acgccgccag cgttcaacac cgatgacgtt agcaccataa cgttcacgta
     3421 atttgagatc gtccagccgt tgaccaatca ttggcgatcc ggggcgaata gccagacgtc
     3481 gcgcacgccc ggtcagtcga tattcacgga taagatcgcg aaaggttcgg cgcgtccagc
     3541 cttcgcgctg cggggtctgg gtatcccctt tcagcatgaa acgcatcact aacatataca
     3601 aaatacccag caccagcacg accaggccaa taggtgttac gctaaagaaa ctgaagccgt
     3661 gatagccttc acgcagcaat tcactgttga ctaccaggtt cggcggcgtc gccaccagcg
     3721 tcatcatgcc gcttatcagc ccggcaaaac ttaacggcat catcagacgc gacggcgacg
     3781 tttgcatacg catggcaacg cttaacacca cgggaataaa gatagcaaca acgccggttg
     3841 aactcataaa cgcgccaagc cccgcgacgg tcagcatcaa caaaaccaac attttgattt
     3901 cactgttgcc cgccactttg accagccatg tccccattac agtggcaaca ccggtacgga
     3961 ccaaaccatc gccaataatg aacaaggcgg caatcaggac aacgttagga tcggaaaagc
     4021 cggaaaatac ttcggggacc gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca
     4081 aagcgaccgc atccatacgc actctgcccg tcgcaaacaa gacgatggca acggccagta
     4141 atgaaagaac ccaaatcaat tcaccgttca caacttatcc ttgttaattg agggggataa
     4201 cttgattctg ccataaaaaa gccccgacga gacggggcta aatcatgatc aggtgtttca
     4261 ctgaataata acatcgccat ttggctgttt ggtcacagta atttgctcca gactgtggag
     4321 gactaaatcg acttcattca ggcgcggggt atctgccggg gcattaaccg caataacatg
     4381 gcaacccgcc gccagacctg aaagcacgcc agcgggagca tcttccacca caacacactc
     4441 ctgcggcgca agccccagca gctgcgcgcc taacagatac gcatcaggtt ctggttttcc
     4501 gcgcttcacc cgctcagcgg ttacaaacac ctctggcgcg ggaagccccg ctattttatg
     4561 acgcgctcgc gctaccggca tggagccaga agtcacaata gcccacggaa tacctgcttt
     4621 attcaaatga ttgagtaagg cgattgcccc cggaagcgcg gtaatgcctt cggtttccgt
     4681 ggcctcaatg tgctccagac gcgtaaactc ggcggcaatg tctgcctcgg atttgcccgc
     4741 cataaaatgg cgcagagagg tgatcgcctg tttaccgtga atgaaagcca gcacctcttc
     4801 cggcgataac ccatgacgtc tggcccagtt gctccacgcc cgttctaccg caggcaggga
     4861 atccaccagc gttccatcaa gatcaaacag aaaacctttg caccgcacgc gggcctcctc
     4921 aggcgttaat aatttgatta atttcgttgg cgctcaaatg gtactgacgc gggcaggcat
     4981 gccagacatt aagcatgcgc tgatattttt cccacattgg cgtctgggcg ttaaaaccgt
     5041 gagttccagc gtcaaaatgg gtatagcgcc cttccacatt aaccataaag cggacataac
     5101 cgaggtaacg tgcttcagtg gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga
     5161 tggattgctg gtcctgcaaa ttagaccagg aaacatgcaa cgcatgatac atctccataa
     5221 tgtcgatgat agtgcggcag gtttcttctt tcagctcgcc aaactcgcga tccagttcac
     5281 gcatctgtaa tccataacca cgctcaatga ttgtttgcag gcgacggtaa cgttcagcat
     5341 ttgccggatc gagcatagtc atcatcttgt actggttaga caaaataaga cgttgcgcgt
     5401 tggtcatttc cattattgac tcctgtatca ctctactacg gtgaaaaaaa agaaggctga
     5461 gtatgccttc ttttatatgc gtaatcaggg gtcaattaca aatcatcaag gaaagtttta
     5521 tccagttgtt tgaaggcacg cttaagcgtg tcagctaatg cctggtaatc aggcttgcct
     5581 tcaacgggtg ccaacacctg tccagactcc tgcaatttac cgcgaacttc ataaaaccag
     5641 ttgaggattg caggaggtaa tggcgttaca gaacgcttgc ccagccacca caatccctgc
     5701 atgggtaaac ttaaggcgaa cagcgcagtg gcaactgccg gcccaagctg accgcccagg
     5761 gcaatctgcc agcagagagt aaatacggcg atcggcggca taaaacggat cgcataacgc
     5821 gtcatcttga taacgcgatt ttcgacaaag actggggcaa ggcgtttttc cagcggccac
     5881 gtctttgagt aatgctgtcc ccggcgaaac aagctaaaaa aattaacaga acgattatcc
     5941 ggcgttgaca tgcttcacct caacttcaca tataaagatt caaaaatttg tgcaaattca
     6001 caactcagcg ggacaacgtt caaaacattt tgtcttccat acccactatc aggtatcctt
     6061 tagcagcctg aaggcctaag tagtacatat tcattgagtc gtcaaattca tatacattat
     6121 gccattggct gaaaattacg caaaatggca tagactcaag atatttcttc catcatgcaa
     6181 aaaaaatttg cagtgcatga tgttaatcat aaatgtcggt gtcatcatgc gctacgctct
     6241 atggctccct gacgtttttt tagccacgta tcaattatag gtacttccat gtcgagtaag
     6301 ttagtactgg ttctgaactg cggtagttct tcactgaaat ttgccatcat cgatgcagta
     6361 aatggtgaag agtacctttc tggtttagcc gaatgtttcc acctgcccga agcacgtatc
     6421 aaatggaaaa tggacggcaa taaacaggaa gcggctttag gtgcaggcgc cgctcacagc
     6481 gaagcgctca actttatcgt taatactatt ctggcacaaa aaccagaact gtctgcgcag
     6541 ctgactgcta tcggtcaccg tatcgtacac ggcggcgaaa agtataccag ctccgtagtg
     6601 atcgatgagt ctgttattca gggtatcaaa gatgcagctt cttttgcacc gctgcacaac
     6661 ccggctcacc tgatcggtat cgaagaagct ctgaaatctt tcccacagtt gaaagacaaa
     6721 aacgttgctg tatttgacac cgcgttccac cagactatgc cggaagagtc ttacctctac
     6781 gccctgccgt acaacctgta caaagagcac ggcatccgtc gttacggcgc gcacggcacc
     6841 agccacttct atgtaaccca ggaagcggca aaaatgctga acaaaccggt agaagaactg
     6901 aacatcatca cctgccacct gggcaacggt ggttccgttt ctgctatccg caacggtaaa
     6961 tgcgttgaca cctctatggg cctgaccccg ctggaaggtc tggtcatggg tacccgttct
     7021 ggtgatatcg atccggcgat catcttccac ctgcacgaca ccctgggcat gagcgttgac
     7081 gcaatcaaca aactgctgac caaagagtct ggcctgctgg gtctgaccga agtgaccagc
     7141 gactgccgct atgttgaaga caactacgcg acgaaagaag acgcgaagcg cgcaatggac
     7201 gtttactgcc accgcctggc caaatacatc ggtgcctaca ctgcgctgat ggatggtcgt
     7261 ctggacgctg ttgtattcac cggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg
     7321 tctctgggca aactgggcgt gctgggcttt gaagttgatc atgaacgcaa cctggctgca
     7381 cgtttcggca aatctggttt catcaacaaa gaaggtaccc gtcctgcggt ggttatccca
     7441 accaacgaag aactggttat cgcgcaagac gcgagccgcc tgactgcctg atttcacacc
     7501 gccagctcag ctggcggtgc tgttttgtaa cccgccaaat cggcggtaac gaaagaggat
     7561 aaaccgtgtc ccgtattatt atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg
     7621 tcagccttgg cgtgatccgt gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac
     7681 ctatcgctca gccgcgtacc ggtggcgatg cgcccgatca gactacgact atcgtgcgtg
     7741 cgaactcttc caccacgacg gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc
     7801 tttccagcaa tcagaaagat gtgctgatgg aagagatcat cgcgaactac cacgctaaca
     7861 ccaaagacgc tgaagtcgtt ctggtggaag gtctggtccc gacacgtaag caccagtttg
     7921 cccagtctct gaactacgaa atcgccaaaa cgctgaacgc agaaatcgtc ttcgttatgt
     7981 ctcagggcac tgatactccg gaacagttga aagagcgtat cgaactgact cgcaacagct
     8041 tcggcggtgc aaaaaacacc aatattaccg gcgttatcgt taacaaactg aacgctccgg
     8101 ttgatgagca gggtcgtacc cgtccggatc tgtccgagat ttttgacgac tccaccaaag
     8161 caaaagtgaa caacgttgat ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg
     8221 gcgctgtgcc gtggagcttt gacctgatcg cgactcgtgc gatcgatatg gctcgccacc
     8281 tgaatgcgac catcatcaac gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt
     8341 tctgcgcacg cagcattccg cacatgctgg agcacttccg tgccggttct ctgctggtga
     8401 cttccgcaga ccgccctgac gtgctggttg ccgcttgcct ggctgccatg aacggcgtag
     8461 aaatcggtgc cctgctgctg actggcggct acgaaatgga cgcgcgcatt tctaaactgt
     8521 gcgaacgtgc tttcgctacc ggcctgccgg tatttatggt gaacaccaac acctggcaga
     8581 cttctctgag cctgcagagc ttcaacctgg aagttccggt tgacgatcac gagcgtatcg
     8641 agaaagttca ggaatacgtt gctaactaca tcaacgctga ctggatcgat tctctgactg
     8701 ccacttctga gcgcagccgt cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac
     8761 ttgcgcgcaa agcgggcaaa cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta
     8821 aagcagccgc tatctgtgct gaacgtggta tcgcaacttg cgtactgctg ggtaatccag
     8881 cagagatcaa ccgtgttgca gcctctcagg gtgtagaact gggtgcaggc attgaaatcg
     8941 ttgatccaga agtggttcgc gaaaactatg ttggtcgtct ggtcgaactg cgtaagaaca
     9001 aaggcatgac cgaaaccgtt gcccgcgaac agttggaaga caacgtggtt ctcggtacgc
     9061 tgatgctgga acaagatgaa gttgatggtc tggtttccgg tgctgttcac accaccgcaa
     9121 acaccatccg tccgccgctg cagctgatca aaactgcacc gggcagctcc ctggtatctt
     9181 ccgtgttctt catgctgttg ccggaacagg tttacgttta cggtgactgt gcgatcaacc
     9241 cggatccgac cgcagaacag ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg
     9301 ccttcggtat cgaaccgcgc gttgctatgc tctcctactc caccggtact tctggtgctg
     9361 gtagcgacgt agaaaaagtt cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgatc
     9421 tgatgatcga cggtccgctg cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca
     9481 aagcaccgaa ctctccggtt gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca
     9541 ccggtaacac cacctacaaa gcggtacagc gttctgctga cctgatctct atcggaccga
     9601 tgctgcaggg tatgcgcaag ccggttaacg acctgtcccg tggcgcactg gttgatgata
     9661 tcgtctacac catcgcgctg actgcgattc agtctgcaca gcagcagtaa tctcgtcatc
     9721 atccgcagct ttgcgctgcg gatatctgaa ccggaaatag tcactatttc cggtttttta
     9781 ttctcttaat ttgcattaat cctttctgat tatcttgctt aactgcgctg catcaatgaa
     9841 ttgcgccatc tcactttgca tacttaccac tttgttttgt gcaagggaat atttgcgcta
     9901 tgtccgcaat cactgaatct aaaccaacaa gaagatgggc aatgcccgat acgttggtga
     9961 ttatcttttt tgttgccatt ttaaccagcc ttgccacctg ggtagttccg gtgggcatgt
    10021 ttgacagtca ggaagtgcag tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag
    10081 atccacactc atttcgcatt ctgactaacg aagcaggcga acctgagtat caccgcgtac
    10141 agctgttcac gacgggcgat gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa
    10201 cctcaggatc gaaatacggg acagccgttg gcatcatcat gtttatgctg gtgattggcg
    10261 gcgcgtttgg cattgtgatg cgtacaggaa ccattgataa cggtatcctg gcgcttattc
    10321 gccatacccg cggaaatgaa attctcttta ttcctgcgct gtttattctg ttttcacttg
    10381 gcggcgcaat atttggtatg ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc
    10441 taatggtccg gctgggctat gacagtatta ccaccgtcct tgtgacctat attgccacgc
    10501 aaatcggttt tgccagttcg tggatgaacc cgttttgtgt ggttgttgct cagggtattg
    10561 ccggcgttcc ggtgctttct ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc
    10621 tgattggcct gatctttacc atggtgtacg cctcacgagt gaaaaagaat cctcttctgt
    10681 cacgcgtgca tgagtccgac cgcttctttc gtgaaaagca ggctgatgtt gaacaacgtc
    10741 cgtttacctt tggtgactgg ctggtattga ttgtcctgac cgccgtaatg gtctgggtta
    10801 tttggggcgt gatcgttaat gcctggttta ttcctgaaat tgccagccag ttcttcacca
    10861 tgggtctggt gattggcatc atcggcgttg ttttccgcct taacggcatg acggttaata
    10921 ccatggcttc atcctttacc gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg
    10981 gtttcgccaa agggattttg ctgctggtcg gtaatggtga agcgggtgat gccagcgtgt
    11041 taaataccat cctcaacagc attgccaatg ccattagcgg tctggataac gcagtcgcgg
    11101 cctggtttat gttgctcttc caggcggtat ttaatttctt cgtgacgtcc ggttctggtc
    11161 aggcggcgtt aaccatgccg ttactggcac cgcttggcga tctggtcggt gttaaccgtc
    11221 aggttaccgt gctggctttc cagtttggcg atggcttcag ccacatcatt tacccaacct
    11281 cagcttcgtt aatggcaacg ctcggtgttt gccgggtgga cttccgtaac tggctgaagg
    11341 tgggtgcgac cctgcttgga ctgctgttta ttatgtccag cgtcgtggtg atcggcgctc
    11401 agttgatggg ctaccactaa aaatgttaag agccgcaatt gcggctcttt tttcattctg
    11461 ccgtttcagt ctctacggct tcatttttgg cattgcgctt catccacaac gccagcgctt
    11521 tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc
    11581 aaacttcact gacttcatct tcctgtagag cgaagggacc gtgagagacg cagctgaaca
    11641 acgcgcccca gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg
    11701 ggaccccggc aatgcccaac tcttcttccg cttcgcgacg cgcggattcc agcagttgct
    11761 catcggcctg gactacaccg cctgcggtcg catctaacat gccgggtaaa aagtcttttg
    11821 tctcggtacg acgctggacc agaattttgc ccatgccatc atgcacgacg atataagttg
    11881 cacgatgacg cagacactgt gcccgcattt gttcccggct ggcttgtgca atgacttcgt
    11941 tctcttcatt gacaatatcc acccattcag tacttgccaa acgacgctgt tccattatca
    12001 ggaaaccttc tttttctggc gctcttacgg cgcattttga gttgtgggta aattacggat
    12061 taatcgcgac ctgcgcaatg atactttgat cattgagtgc gattacacta agtacgtcat
    12121 tatccagcat gccataactc gccggattac cgcctttcgg aatactcacc gagccggggt
    12181 tgaagtggaa aatctccccc cgctgttctg ccactggcag atgggtatga ccgtacacca
    12241 gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc aaaaagatga ccatgcgtca
    12301 aaaacagacg ttgtttttcc agtaatacct gttgccacgg cgcggttatc gggaaatgca
    12361 gcagcatttg atccacttcg ctgtcgcagt tgccgcgcac agcgataacc ttatgtgcca
    12421 cttcattaag ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa gcattacgcg
    12481 ggccatgatt caacacgtcg ccaaggatca ccagccactg ggcaccgctt tggacaaaca
    12541 actccagaac acgttccgtc gccggtaacg acccatgaat gtccgatgca aacatcagtt
    12601 tcatcactca ctcctcgtcg aaaaaacgta agctatgata ctggattctg tcgttgctat
    12661 cagccagatt gcttcgccga aagtgcaata aaacgctgga cagacgctcg ctgccactgg
    12721 aatgtcgcat aatccaccag gcgttccggc acctcatcgc catgtagtac caggcggtta
    12781 atcattagcg ccagatcagt atcagcaatg caccattcac caaataaatt cggctgaccc
    12841 agtgctaaca aatgttctgc catcgcgaac agtttctctg cactggcttt tccctcggcc
    12901 gttagtggcg cttttttcgc ccccgcaaag acaacatccg tcgggcgttc ttcgcggatg
    12961 ggcatcagat cgctgcgcaa ccaagcctga atctgtcgtg cacgcgcacg attttctaaa
    13021 tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag atactccgca
    13081 atggcagaag attcactcaa ctcaaaatca tcgatttgta ataacggcac acggcgtgtc
    13141 tgaccgtaac cttgccacgt cggctgcaaa tgttcactgc tgtcgaggtc gatggtcttg
    13201 atatgaaacg acaggccttt ctcctgcaac gccacccagg cggataacac ataaggggag
    13261 aaaaaatggg catctgacca aagcgtgata gcggttttac tcattatgtc ctcggcaata
    13321 aagggggctt gctatcaaac tatagagcct ttttgtcact gtcacgtgac gaatactcac
    13381 gcaattcgcc gcgctttcta tacttgtggt gttcagatga ttagacaacc ggagctgcaa
    13441 tgatcgatct ctattttgcc ccgacaccca atggtcacaa aattacgctg tttctcgaag
    13501 aagcagggct ggattatcgc ttaataaagg tagacctggg gaaaggaggt cagtttcgcc
    13561 cggaattttt gcgcatttcg cctaacaaca aaattccggc aattgttgat cattctccag
    13621 ccgatggcgg cgaaccgcta agcctctttg agtctggtgc cattttgttg tatctggctg
    13681 agaaaacggg actctttttg agtcatgaaa cgcgtgaacg cgccgtcaca ttacagtggt
    13741 tattctggca ggtaggcgga ctggggccga tgcttgggca aaatcatcat tttaatcacg
    13801 cagcccccca aaccattcct tacgctattg aacgttatca ggttgaaact cagcgtctgt
    13861 accatgtact gaacaagcgg ctggaaaatt cgccctggct gggaggcgag aactacagca
    13921 ttgcggatat tgcctgctgg ccgtgggtta atgcctggac tcgccagcga attgacctcg
    13981 caatgtatcc ggcagtcaag aactggcatg agcggatccg ttcgcgccct gccaccgggc
    14041 aggcactgct aaaagcacaa cacggtgatg agcgttcgga tagttaacag aaacaggttc
    14101 tcgtgtatta tttcattcta agtaaaacaa cggagaacct gcaatggcac aacctgccgc
    14161 tattattcgt ataaagaacc ttcgtttgcg tacgtttatc ggaattaagg aagaagaaat
    14221 taacaaccgt caggatattg ttatcaatgt gactatccac taccccgccg ataaagcgcg
    14281 cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa acattattca
    14341 gcatgtagag aataaccgtt tctctttgct ggaaaaatta actcaggatg tgctcgatat
    14401 cgcacgtgaa catcactggg tgacgtatgc tgaagtggag atcgataaac tgcacgcgct
    14461 gcgctacgcc gattcggtat ccatgacctt aagctggcag cgttaaccgc catatcggga
    14521 ggctgcatga acatagtgat caccggaggg acgggattaa ttggtcgcca tttgattcca
    14581 cgtttgctgg acctgggcca tcaaatcacg gtagtgacgc gtaacccgca gaaagccagt
    14641 tccgttctcg gccctcgggt gacactatgg caagggcttg ccgaacaaag caacctcaac
    14701 ggcgttgatg cggtaatcaa cctggccgga gaaccgattg ctgataaacg ctggactcac
    14761 gagcaaaaag agcgtctctg ccaaagccgc tggaatatca cgcaaaaact ggtcgatttg
    14821 attaatgcca gcgacacgcc gccgtcggta ctcatttccg gctcggcaac gggctattat
    14881 ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga atttacccat
    14941 aaactctgcg cccgctggga agagattgcc tgtcgggcgc aaaatgacaa aacgcgagtg
    15001 tgcctgctgc gtaccggcgt agtgctggca ccagacggtg gtatcctcgg taaaatgctg
    15061 ccgccatttc gttttggcct gggcgggccg attggctctg gtcggcagta tctggcctgg
    15121 attcatattg atgatatggt caacggtatt ctctggctgc tggataacga gctgcgcggg
    15181 ccatttaata tggtttcgcc ctacccggtg cgcaatgaac aatttgccca tacactgggt
    15241 catgcgctgc atcgcccggc cattttgcgc gtccctgcga ccgccattcg gctgttaatg
    15301 ggcgaatctt cagttctggt attaggcggt caacgcgcgc tgccaaaaaa gctggaagaa
    15361 gcgggttttg cgtttcgctg gtacgattta gaagaggcgc tggcggatgt cgttcgctga
    15421 tggggtttac tgcaaacatc cgccagttaa ctcccggtgt tacagggtta gtggctttgc
    15481 gcgataagat cgtctggtga aagtcgggtc agcgtcagga tgacttcgtg gtcaaatcct
    15541 ttttccatca tctcctgagc aatgcgcagg gcttcttcgt gtttgccctg cattgcgcct
    15601 tcttcacgta atctgtcagc aatggtcatc agtttctcct tttcttgtgg tgcgcgttcc
    15661 gctatctcac caataaatgc acggaaacgc cgggcatccc ccgtttgtaa tacgtaatta
    15721 aacagggctt ttagctgtct gtcattagtg ttccctgtaa ctagcagcga aacaatttgg
    15781 tcgactaatc ccaacagatc gcgctgacga atatgtttct gaattaactc caacagcgcc
    15841 attttgcgat gttgcataat ctcgtcatcc ggcaccacgg taatatccac cagcggaaaa
    15901 gccgatgaat aaattttgcg ggcaatcgca ggctcggcaa attcatcaag ccagcagagt
    15961 gaatatggat aagggcttct gcaaccatga taaaacagca tcgggatcac taatggaagc
    16021 tctttataac ccgcatcaag atggttttgc atcgccgcaa tggaatagcg catcatgcga
    16081 aaagccatta attcctctgg cttactttgg tgctctatca ccacataaat ataacccgct
    16141 ccctcctgcg ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca
    16201 ataaaactgt ttggttccag tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg
    16261 ggaagatgaa tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat
    16321 accgcatcat gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat
    16381 ttaagatgac gcgacaataa ccacgattta cacccaatgc agcaggcagt ttttatcttt
    16441 ggacagcgaa ttcagaacta tttttagcgg actgcaactt ttacatttca gcggaagcgg
    16501 actcgcaaat tccccatgaa atactccggc agcgttaacc accggagagg aaattatttc
    16561 agcgaacctt taaggaactg ttgcagacga gggctttgcg ggttaccgaa taactgctcc
    16621 ggcgcccctt cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatga
    16681 cgagcaaagc ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc
    16741 tgcataatac gcaacacttc gcctaccagt tcaggatcga gcgctgaagt aggttcatca
    16801 aacagtaaaa cttccggttc catcgctagc gcccgcgcga tagaaacacg ctgttgctga
    16861 ccaccggaca ggtgcaccgg gtatttcccc tgcgcacgtt cgtctatccc gacttttgcc
    16921 agatatttca ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc
    16981 gcttccatga cgttttccag caccgtcata tggctccaga gattgaagtg ctggaatacc
    17041 atcgtcaggc gtgtgcgcaa taagcgcagc tgatttttat cggcgacttt gagttgaccg
    17101 tctttatcgc gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcgctcggt
    17161 ttttcgagga agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg
    17221 atgcttatga catctccggc attcgcttgc aatgagaccc ctttcagcac ttcgtgttcg
    17281 ccgtagcgtt tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg
    17341 cgttgaagaa ggtttcacat gctgcaacca gcgtttttca gctctacgaa agaggctgat
    17401 caggacataa gagatgatta aatagagcac cgcggcaatg ccaaaagcgg taaaaggttg
    17461 atatgtggcg gcgttaatat cgcgggcaat tttcagcaga tccggcaccg tggcagtaaa
    17521 tgccagcgca gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat
    17581 acgcagcgca gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc
    17641 tctggcggct tcaatttccc catgcggcac tgaacggatt gccccggcaa aaatctcggt
    17701 agtgtaagcg caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcgaaa
    17761 aaaggcgtta aggaactcag ttcccttaac aatctccagc gtgtacatgc cggaatagaa
    17821 caccagcaac tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg
    17881 aaactggatg tatttattac ttgagacgcg accaatcgcc agaaacagcg ccagaactcc
    17941 gcctatcact accgacaaaa tcagcagcca cagggtgatt gctacgccag taaagcgata
    18001 accgtcagtc cacagcagtg gtttccagta ttcatgtaag atttcgatca caggtcagcc
    18061 ctcttcacac ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg
    18121 gtgaaaacaa gataaatcac gccgcagacg atagcgaaat agaacggttc ccaggtactt
    18181 ttgcctgcca gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg
    18241 gtagatttga ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc
    18301 gccggaaaca tgatccgccg aaacacttgc ccacgagtaa aaccgaacgc cgtcgccgcc
    18361 tctatatgtc ctttcggcac tgccataaac gcgccacgaa acgtttcggt aaaataagca
    18421 ccgtagataa aaccgagagt gataatacct gcgaccatcg gatcaatatc aatctgcccg
    18481 acgcccatcg cctccgtcac cgtgtttagc gcaatctgca aaccgtagaa aatcagcagc
    18541 atcaatacta aatccggcac gcctcgaatc agcgtggtgt acccttcgaa aataaggccc
    18601 gaaagccgat tttgtgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc
    18661 actacagagc tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa
    18721 aacccataca acatactgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc
    18781 tccggatacc cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat
    18841 caaaatcgaa gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa
    18901 aggctttgtt cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc
    18961 ctacgccaaa cagtttttca tctttaacag acgggccacc gaatttgtaa tctttaccga
    19021 cgggttgttt gaggaaacct tcgctggctg cgacctcatc ctggaacgcg gcatcaatac
    19081 gtccggcagt caggtcagaa taaatattgt cctgcccctg ataagaaacg atttcaatgc
    19141 cttttggtgc ccagtgttcg ttaccaaatg tctcctgggt ggtgccctgc aatacgccta
    19201 cccgtttgcc tttcagcgat tcgactgtcg gctgaatgtc ggaatttttc gccaccacca
    19261 gacgagaatc ggcagcgtac agtttgtcgg tgaaggctat ttcttgctga cgtttttccg
    19321 taatggaaag cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg
    19381 catccagtgg gttttcgaca aacgtacatt gcgtattgat gcgtttgcac aactccttcg
    19441 ccagatcgat atcgaagcca accagttcac cttgtgaatt ttttgattca aatggcgcat
    19501 aggtcggatc ggtaccgatg cggatgtttt gcggaatcgc agcaaatgcc gcagttgcgc
    19561 tggagaaggc cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg
    19621 acagtctttt tatcgggaat atttacaggt ttgttacact tatcgtgcca taaaattgac
    19681 catcaaggca aaatatcccg cccctccaga cgtattattt ttcattaaaa atgcttaagt
    19741 atgcatttca atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc
    19801 atcaggtaca gcttccctgc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt
    19861 ttcgccatct tgtcgtaggt gccgtcctga cgcagctcgt taagcgcctt attgaaggcc
    19921 gccgtcagtt cagcatcatc tttacgcagc cctacaccgg tgccgtcacc gaagtatttt
    19981 ttgtctttca ctgatgggcc tgcaaaggcg aaatctttac cggcgggttg cttaaggaaa
    20041 ccttcgctgg cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg
    20101 gaatagacca aatcctggtt ggcataggcc accacatcca cgcctttgct acgccaggtc
    20161 tcgttagcgt atgcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt
    20221 gaatccagcg ttggctgaat cggtgaacct ttggcggcaa tcaaacgaga atctgcggcg
    20281 tacagcttgt cagagaaggc tatctcctgc tgacgtttat cggtaatgga gagcgacgaa
    20341 ataatgacgt cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca
    20401 acccaggtac atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag
    20461 ccaacaaagt cacctttggc atctttcgat gagaacggtg cgtaggtggt atcggttccg
    20521 atacgtaccg tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaac
    20581 aaagacagag cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta
    20641 tgttgtgtat tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac
    20701 gacagcagta acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctaaagt
    20761 tgaagatagc aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat
    20821 gtcgaggatt tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc
    20881 gccctgccag cgggcaaaga gatcttcagg aagggttacc gcaaactggt caagaacacg
    20941 attaaccgtc tgatttatca catcatcaag ggattgtggg cgatgataaa acgccggaac
    21001 gggaggcata atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa
    21061 gtgcagtggc gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc
    21121 tgccgcacgg gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat
    21181 tgaacagggt aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tactggcggc
    21241 aatatcgcgc gcatcatggg tgacatcggc taatgcctgc acttcgcgta gggaaaaatc
    21301 cgtttcgaga gataaagtct ggcgggctgc ctgactcatc accagatgcg tttcgatatc
    21361 tgtgacatcg cgcagaactt gtaataaacg cacgccataa atcgcgccac tggcaccgct
    21421 gatgcccaca atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg
    21481 caggatttcg acgggagttg caagtcaggg tgccagaccg gcaccctgag cgaaggtgtt
    21541 atccttcgtt atgcatttcg agattttcca cttcgttctg acgttgcacg gctttggcat
    21601 cgtcattacg taaagtatca agaaaatcga gatagctctg atcaacatct ttggtgacat
    21661 agacaccgtt gaacaccgag cattcaaact gctggatatc cgggttttca gcgcgaacgg
    21721 cttcgatcag atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa
    21781 tttcatctac ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcgataccgt
    21841 aaacgttcgg gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg
    21901 cttcgcgtgc catctcgata atctgctcag acgtggtgcc acggacgata gagtcgtcga
    21961 ccagcaggac gtttttatcg cggaactcgg cgcggttggc gttcagttta cggcgcacgg
    22021 acttacgacg cagctgctga cccggcatga tgaaggtgcg gccaacataa cggtttttaa
    22081 cgaagccctg gcggtacggt ttgcccagaa tacgcgcaat ttccagcgcg atatcacacg
    22141 aggtttccgg aatagggatc accacgtcga tatccagatc ttcccattcg cgggcaattt
    22201 tttcgcccag tttggtgccc atattcacgc gcgcgctgta aacggaaatt ttgtcgatga
    22261 acgagtccgg gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt
    22321 cagcacactg acgggtaaat aactgccctt cttcagtgat gtaaatcgct tcgcccggcg
    22381 cgacgtcacg caggaaatca aagcccagcg tatcgagcgc aacgctttcg gaagcgacca
    22441 tatattctgt gcggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt
    22501 taggatcgcg gaaagcaacc ataccgtggc cgatgatcat cgccacacag gcatacgcac
    22561 cgcggattaa gcggtttgta gcagcgatgg cggcgaaaat gttgtcggct tccagcgggt
    22621 agtggcggaa gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag
    22681 tggtgttgat gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg
    22741 tcagattgcc gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg
    22801 cttcagaggc gctggagctg ccagccgtgg ggtaacgcac atgaccaatg cccatattgc
    22861 cctgcaaacg ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttcc
    22921 gcaaacggaa gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat
    22981 gctgaagcac cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac
    23041 cgacaatacc gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc
    23101 tgggcaagaa acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga
    23161 actgcgggat cagctgtgat ttgctccagt cttcgctttt cgacaccccg gtaaaggagt
    23221 cgagaaagaa gagaatggca gcaacaatca acacaccgcg caacgcaccg aaacagacgc
    23281 ccaacacccg atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt
    23341 tcacgatagc accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc
    23401 gaaccagttc gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg
    23461 caacaaagaa agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgcgga
    23521 tcaggctaac cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca
    23581 tatgtgtccc acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag
    23641 aaaacgtttg cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat
    23701 tcaacgccat cgacttttta tgcctttgcg gcatcgggca atgcgtgtcg gatgccgcat
    23761 gaacgcctta tccgacctac agttctgctc cagca
//
LOCUS       DAZKHK010000085        23787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKHK010000085 DAZKHK010000000
VERSION     DAZKHK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.085"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(355..2025)
                     /gene="betA"
                     /locus_tag="ACHOS5_003311"
     CDS             complement(355..2025)
                     /gene="betA"
                     /locus_tag="ACHOS5_003311"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP9778080.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAAEKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGIGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2039..3511)
                     /gene="betB"
                     /locus_tag="ACHOS5_003312"
     CDS             complement(2039..3511)
                     /gene="betB"
                     /locus_tag="ACHOS5_003312"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP9778081.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGCQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADITMM
                     ANFFSSGQVCTNGTRVFVPTKYKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3525..4112)
                     /gene="betI"
                     /locus_tag="ACHOS5_003313"
     CDS             complement(3525..4112)
                     /gene="betI"
                     /locus_tag="ACHOS5_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP9778082.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAERRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKALDKPLAHSLTRHFITQHLPTD"
     gene            4241..6274
                     /gene="betT"
                     /locus_tag="ACHOS5_003314"
     CDS             4241..6274
                     /gene="betT"
                     /locus_tag="ACHOS5_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP9778083.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDS"
     gene            7152..8240
                     /gene="pdeL"
                     /locus_tag="ACHOS5_003315"
     CDS             7152..8240
                     /gene="pdeL"
                     /locus_tag="ACHOS5_003315"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HFP9778084.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(8282..9214)
                     /gene="yahB"
                     /locus_tag="ACHOS5_003316"
     CDS             complement(8282..9214)
                     /gene="yahB"
                     /locus_tag="ACHOS5_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9778085.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLITRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9306..9803)
                     /gene="yahC"
                     /locus_tag="ACHOS5_003317"
     CDS             complement(9306..9803)
                     /gene="yahC"
                     /locus_tag="ACHOS5_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9778086.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLAPVSGISGVWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10061..10666
                     /gene="yahD"
                     /locus_tag="ACHOS5_003318"
     CDS             10061..10666
                     /gene="yahD"
                     /locus_tag="ACHOS5_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9778087.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGANINKQDHTCLNPFLMSCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            10706..11569
                     /gene="yahE"
                     /locus_tag="ACHOS5_003319"
     CDS             10706..11569
                     /gene="yahE"
                     /locus_tag="ACHOS5_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9778088.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITVGQHHHIEMSHCQRWLSPTLQMTAL
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     AVAVASMMGLGIGLTPSADDYLTGLVLVLFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            11559..13106
                     /gene="fdrA"
                     /locus_tag="ACHOS5_003320"
     CDS             11559..13106
                     /gene="fdrA"
                     /locus_tag="ACHOS5_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9778089.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            13106..14524
                     /gene="yahG"
                     /locus_tag="ACHOS5_003321"
     CDS             13106..14524
                     /gene="yahG"
                     /locus_tag="ACHOS5_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9778090.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKITAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAIFGWCERYGV"
     gene            14548..14832
                     /locus_tag="ACHOS5_003322"
                     /pseudo
     CDS             14548..14832
                     /locus_tag="ACHOS5_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14864..15814
                     /gene="yahI"
                     /locus_tag="ACHOS5_003323"
     CDS             14864..15814
                     /gene="yahI"
                     /locus_tag="ACHOS5_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP9778091.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPIKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            15824..17206
                     /gene="yahJ"
                     /locus_tag="ACHOS5_003324"
     CDS             15824..17206
                     /gene="yahJ"
                     /locus_tag="ACHOS5_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9778092.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGPSVPLAHAGVAGTLNCEAN
                     NTMKITDPYYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEALMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            17506..17916
                     /locus_tag="ACHOS5_003325"
     CDS             17506..17916
                     /locus_tag="ACHOS5_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9778093.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            18170..19153
                     /locus_tag="ACHOS5_003326"
     CDS             18170..19153
                     /locus_tag="ACHOS5_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9778094.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMENLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            19202..20686
                     /locus_tag="ACHOS5_003327"
     CDS             19202..20686
                     /locus_tag="ACHOS5_003327"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9778095.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLSFLMTGQEFEYQERELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQLVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     AVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            20679..21650
                     /locus_tag="ACHOS5_003328"
     CDS             20679..21650
                     /locus_tag="ACHOS5_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9778096.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            21647..22603
                     /locus_tag="ACHOS5_003329"
     CDS             21647..22603
                     /locus_tag="ACHOS5_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9778097.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGVNILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFTAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            22690..23739
                     /gene="yahK"
                     /locus_tag="ACHOS5_003330"
     CDS             22690..23739
                     /gene="yahK"
                     /locus_tag="ACHOS5_003330"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP9778098.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHDIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         5604 a         5848 c         6455 g         5880 t
ORIGIN      
        1 aataaattat acgaatcgtg tttaaagctc catgtggttt tggtctgact tatttttgta
       61 ctcgttacaa gaaccctttt cgtatatgct aaccgtgagc gggaatgaac aggtaaccat
      121 agccaggaac attagcggcg taattcttaa gctgagtggt atttcatctg tagtttatct
      181 gtgcattcat agcaccttca tcagccagat tgagcaagtc agcttagaat ttgctggcga
      241 gattgtcacg atgaagcatg agagttacct cagtgtttta tataaggatt cggtcctcat
      301 agtagaacgg taactctcaa tctgataggt tcctgagtca gttcacatca cgactcattt
      361 tttcgctctc accggcatcc catttgccac aaaatatccc gccgtgctcc tcggcaacgc
      421 ttccttcccg cgaatcatat ccgctatttt ctcgccaatc ataatcgtcg tggcgttcaa
      481 attgccggtg ataatttgcg gcataattga cgcatccacc acgcgcaggc cttccagccc
      541 gtgtacgcgg ccttcgccgt caaccacagc catctcgtca taacccattt tgcaggtacc
      601 gcacggatga aacgcggttt cggcgtggtt acgcacgaac tcatcgagct gctcatccgt
      661 ctggcattcg acaccagggc tgatttcgcg gccacggtat tgatccagcg cgggttgatg
      721 catgatctcg cgggtgatgc gaattgcgtc gcggaactcc tgccagtcct gctcgtgcga
      781 catgtagtta aacagaattc ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg
      841 cccacggctt ggcgagcgca tcgatccgac atggcactgg aagccgtgct ctttcactgc
      901 gttcgagccg ttatagttaa tcgccactgg caggaaatgg tactgaatat tcggccacgc
      961 aaattcctca cggctgcgaa taaatccgcc tgcttcaaag tggttgctgg caccaatgcc
     1021 ggtgccgcca aacagccact ccgcaccgat tctcggctga ttccaccact gcagagcagg
     1081 gtagagggaa accggttctt tgcactcgta ttgcagatac atctccaggt gatcctgaag
     1141 attttcgccg acgccgggta aatcatgcac cagcggaata tcaaactccg ccagcagttc
     1201 agcgttgccg acgccggagc gttgcaggat ctgcggtgaa gcaatcgcgc ctgcacataa
     1261 cagcacttct ttttccgccg ccgcgcgggt cggaatggtg ctgtcgcctt ccagccactc
     1321 gacgcccacc gcgcgtttgc cgtcaaaaat gatgtgatcg gtcatggcgt gagttcgaat
     1381 agtcaggttc gggcgcgatt tggcctgatc gagataaccc cgcgcggtgc tggcgcgacg
     1441 gccctgtggc gtgacggtgc ggtccatcgg gccgaaacct tcctgctgat acccgttaag
     1501 gtcgtccgtg cgcggataac cggcctgcac gcccgcctca atcattgctt caaacagcgg
     1561 attgacacca ggttttgagg tggtcacgct taccgggcca tcaccgccgt ggtagtcgtt
     1621 ctcgcccaca tcgcgcgtct cggccttgcg gtagtagggc aggcagtcga ggtagctcca
     1681 gttctccaga ccgggttctt tcgcccagtt atcgagatcc agtgcgttac cacggatgta
     1741 acacatgccg ttgatcagtg atgatccacc aagccctttg ccgcgtccgc actccatgcg
     1801 acggttgttc ataaacggtt cgggttccgt ctcataggcc cagttatagc gtttgccctg
     1861 tagcgggaac gccagcgcgg cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc
     1921 gcctgcttca agcagcagca cggttgtgtt cggatcttca gtcagacggg tagcgagaac
     1981 gttgccggct gagccggcac caataatgat gtagtcaaat tgcaaataaa cctcctggtt
     2041 agaatatgga ctggaattta gccatctcaa cctggatgga cttcacctgg gtgtaactct
     2101 ggagcgtcat cacgccgttc tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca
     2161 tctctgccgg ggattcgccc caggtgttga tccagcaaat acccgcttcc agctgatgaa
     2221 tgacgcgatg cgcacggttc aggtccgccg tcacgatacc cgccgccaga ccgtagtcgg
     2281 tatcgttagc gcggcgaatg acttcgtctt ccgactcgta ggtcagaatg gacatcaccg
     2341 gcccgaaaat ctcttctcgc acaatggtca tatcgtcgct gcaatcggtg aacacggtcg
     2401 gtgcaaccca tgcgccgtta tcgaagctat cccccttcag tacattgccg ccgcacagta
     2461 cgcgcgcgcc ttcctctttg cctttggcga tatagcgcag cacgttatcg cgatgcggga
     2521 agctgaccag cgggccgaag ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct
     2581 caacgcgcgc cagaatcttt tgttcaaatg cggctttgta tttcgtcgga acgaagacgc
     2641 gagtgccgtt ggtacacacc tgaccggagc tgaagaaatt cgccatcatg gtgatatcgg
     2701 cggcgagatc gagatccgca tcgtcgaaaa cgatcagcgg tgatttaccg cccagctcca
     2761 tggtcacttc tttcagggag gaagctgccg agttagccat cacttttttg ccgctggcga
     2821 caccgccggt aaatgacact ttggcaatgc ccggatgatc ggtcagatat tgcccggtct
     2881 ccgcgcccac gcccggcaac acgttaaata caccgtccgg caggcccgct tcgctgtaaa
     2941 tttcagccag ctttaacgcg gtaagcgggg tgacttcgct cggtttgaag atcatcgcgt
     3001 tacccgccgc cagcgcgggg gcggatttcc acaaagcaat ctggatcggg tagttccatg
     3061 cgccaatccc cgccaccaca cccagcggtt cgcggcgggt ataaacaaat gacgtctcac
     3121 gcaacgggat ctggcagcct tccagcgccg ggatcagccc ggcatagtac tccagcacgt
     3181 ccgcaccggt aacgatatcg acggttgagg tttccgaata tgcttttccg gtgtcgaggg
     3241 tttccagttt tgcgagttcg tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa
     3301 tacgcgagcg ttccatagcg gtcatcgccg cccagatttt ttgcccttgt tgggcactct
     3361 tcacggcgcg atcgacatcc tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt
     3421 tggccgggtt aatggtctcg aaggtgcgac cgctggtggc ggaggtataa ccaccatgta
     3481 tataaagctg ctgttctgcc attcgggaca tcacgtttcc tcggttaatc ggtgggtaaa
     3541 tgctgagtaa taaagtgacg ggtcagcgag tgtgcgagag gtttatccag cgctttgccg
     3601 ctcagtgccg cgcgcagcca taatccgtca attagcgcgg caaggccgta gcccgcttcc
     3661 tgtgcctgtt ggcgcggtaa ctcgcggcga aattcgctca ccagattcga caataagcgg
     3721 cggctactga cctgctgcaa gcgatagagc atcggctgat gcatactgct ggcccagaag
     3781 gccagccagg ctttcatcgc cgcactgctc acctgggttt catcgaagtt tccgccaaca
     3841 atcgcctgta atcgccgctc tgcactgccc tgcggaagtg catgtaatcg attcaaaacc
     3901 gcgtcacgca gctgactggt gatatcgcgc atggttgctt ccagcagacc atttttgtcc
     3961 ctgaaatagt ggctgatgat ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc
     4021 gttgcatcgt gcatgcccac ttcatttatt gcttccagcg tggcgtcgat cagttgtctg
     4081 cgccggatcg actgcattcc caatttgggc attttcgcca ctccattcat cagcgttgtt
     4141 tatctattaa agcggttatt gattggacgt tcaatataaa atgtgtctta attgttacta
     4201 atttgatttt aaatggtaac aataacagtg gggatactgg atgacagacc tttcacacag
     4261 cagggaaaag gacaaaatca atccggtggt gttttacact tccgccggac tgattttgtt
     4321 gttttccctg acaacgatcc tgtttcgcga cttctcggcc ctgtggattg gccgcacgct
     4381 ggactgggtt tctaaaacct tcggttggta ctatctgctg gcggcaacgc tctatattgt
     4441 ctttgtggtc tgtatcgctt gttcgcgttt tggttcggtg aagctcgggc cagaacaatc
     4501 aaaaccggaa ttcagtctgc tgagttgggc ggcgatgctg tttgctgccg ggatcggtat
     4561 cgacctgatg ttcttctccg tagccgaacc ggtaacgcag tatatgcagc cgccggaagg
     4621 cgcgggacag acgattgagg ccgcgcgtca ggcgatggtc tggacgctgt ttcactacgg
     4681 cttaaccggc tggtcgatgt atgcgctgat gggcatggcg ctcggatact ttagctatcg
     4741 ttataatttg ccgctcacta tccgctcggc gctgtacccg atcttcggta aacggattaa
     4801 cgggccgata ggtcactcag tggatattgc tgcggtgatc ggcaccatct tcggtattgc
     4861 cactacgctc ggtatcggtg tggtacagct taactatggc ttgagcgtgc tgtttgatat
     4921 tcccgattcg atggcggcga aagcggcact gatcgccttg tcggtgatca tcgccactat
     4981 ctcggtgaca tccggtgttg ataagggcat tcgcgtgtta tcggaactca acgttgcgct
     5041 ggcgctggga ttgattctgt tcgtgttgtt tatgggcgac acctcgttcc tgcttaatgc
     5101 actggtgctg aatgttggcg actatgtgaa tcgctttatg ggcatgacgc tcaacagttt
     5161 tgccttcgac cgtccggttg agtggatgaa taactggacg ctcttcttct gggcatggtg
     5221 ggtggcatgg tcgccgtttg tcggcttgtt cctggcgcgt atctcgcgtg ggcgtaccat
     5281 tcgccagttc gtgctgggca cgttgattat tccgtttacc ttcacgctgt tatggctctc
     5341 ggtgttcggc aatagcgcgc tgtatgaaat catccacggc ggcgcggcat ttgccgagga
     5401 agcgatggtc catccggagc gcggcttcta cagcctgctg gcgcagtatc cggcgtttac
     5461 ctttagcgcc tccgtcgcca ccattactgg cctgctgttt tatgtgacct cggcggactc
     5521 cggggcgctg gtgctgggga atttcacctc gcagcttaaa gatatcaaca gcgacgcccc
     5581 cggctggctg cgcgtcttct ggtcggtggc gattggtctg ctgacgcttg gcatgctgat
     5641 gaccaacggg atatccgcgc tgcaaaacac cacggtaatc atggggctgc cgttcagctt
     5701 tgtgattttc ttcgtgatgg cggggttgta taaatctctg aaggtagaag attaccgccg
     5761 tgaaagtgcc aaccgtgata ccgcgccgcg accgctgggg cttcaggatc gcctgagctg
     5821 gaaaaaacgt ctctcgcgcc tgatgaatta tccgggcacg cgttacacta aacagatgat
     5881 ggagacggtc tgttacccgg caatggaaga agtggcgcaa gagctgcggt tgcgcggcgc
     5941 gtacgtggag ctaaaaaacc tgccgccgga agagggacaa cagttggggc atctggattt
     6001 gttggtgcat atgggcgaag agcagaactt tgtctatcag atttggccgc agcaatattc
     6061 agtgccgggc tttacctacc gcgcacgtag cgggaaatcg acctactacc ggctggaaac
     6121 cttcctgtta gaaggcagcc agggcaacga cctgatggac tacagcaaag agcaggtgat
     6181 caccgatatt ctcgaccagt acgagcggca ccttaacttt atccatctcc atcgtgaagc
     6241 gccgggccat agcgtgatgt tcccggactc gtgattgtta ttgcataaaa ccgcgccatg
     6301 tctgcatatg gcgcggtttc tcttacctct tgatacgtta tatctgtgta cagttaagcc
     6361 cttagtatcg attgatgatt gccaggcaac agataataaa aaagaaaaga actattgaag
     6421 cccaaaacct acgtttggac tgttgtgaat gttcaataag cttagtctta tttaatgtaa
     6481 atattggtgc taatttgaaa tgacgcttta ttcatgggga acgcgtagcg caaatcaact
     6541 aaaatcaatg taaccgtttg ttgtttaagg tttaattgtc tgtgtctgat attttattga
     6601 ttgttttaaa tattgttttt attgtcattg ttataatgtt ggctatctat tgctgaatgg
     6661 attcagtctt aaaaagcagg ttgttaaggg ataggcatag agtaatgacg tgtatgcata
     6721 accaacatct ttactcatta tgtcattgaa tgttgacgct atgtgtttat gaggttgagg
     6781 tattttcagt tgatctggat tgttaaattc atataatgcg cctttactca tgaatgggcg
     6841 tcaatacgta gtgggaaatt ataaatatcg aaatagtcca actacttctt tattaccaaa
     6901 aatgagtatc tgaattttaa tattgcattc ttgcgtgatt atctcctgag tttgacttgt
     6961 gattaccttt ttaaggtatt tagcgtaact gtttttgagc gagcatcaga ggtaaagata
     7021 atcttcttga tagtgatgtg ggatgttata cgtatggcat cgctaatgtt tatggttacc
     7081 ccttatgtgt gctcaggaat ccacaggtaa tcactcatac tgaacagcga taaaagataa
     7141 aaggtgtgtt catgaattca tgtgattttc gtgtttttct gcaagagttc ggtacaacgg
     7201 ttcatttgtc attgcctggt agcgtatccg agaaagaacg actgctactc aagctgctga
     7261 tgcagggaat gtctgtaaca gaaatatcac agtacagaaa tcgcagtgca aagaccattt
     7321 cacatcaaaa gaaacagctc tttgagaaac tggggattca gagcgatatt actttctggc
     7381 gggatatttt ctttcagtac aatccggaga tcatatccgc cacgggtaat aatagtcaca
     7441 aatatattaa tgataatcac tatcaccata tcgtcacgcc tgaagccatc agtctggcgt
     7501 tggaaaacca cgagtttaaa ccgtggatcc aaccggtttt ctgcgcgcag actggggtac
     7561 tgacaggctg tgaagtgctt gtccgctggg aacatccaca aacgggaatg atcccaccgg
     7621 atcagtttat tcctctggcg gagtcatccg gtcttattgt cataatgact cgccagctga
     7681 tgaaacagac tgcggatatt ctgatgccgg taaaacattt gctgccggac aatttccata
     7741 ttggtatcaa cgtctcggcg ggctgttttt tggctgcggg atttgaaaaa gagtgtctga
     7801 acctggttaa gaaattaggt aacgataaaa tcaagctggt tcttgagctg acggaacgta
     7861 atcctattcc ggtaacgcca gaagccagag cgatatttga cagccttcat cagcacaata
     7921 ttacctttgc gctggatgac tttggtacgg gttatgcgac ctatcgttac ttgcaggcgt
     7981 tcccggtcga ttttattaaa attgacaagt cgtttgtgca aatggcgagt gtcgacgaaa
     8041 tctccggtca tattgtggac aatattgtcg aactggcgcg taagcctggt ttgagtatcg
     8101 tggcggaagg ggtagaaacc caggagcagg cggatttaat gatcggtaaa ggcgttcact
     8161 ttttacaggg ctatttgtac tctccgccag taccgggtaa taaatttatc tctgaatggg
     8221 taatgaaagc aggtggttga tgtaaaccgc tattcacagc gcatcgggag gttggcagcg
     8281 attaattctc ccgatgcagt aaatcctgat agatccccgt taatatgcca tttggcgcaa
     8341 attgtttttt aatccactgc gtcacctgac cggtggcaga gtgctgggtt gctagtaaca
     8401 tgcgtgaatc ctggcgcggg ttatggattt gtcgggtaat cagcaaggat tgcgccatcg
     8461 cctcacggac catgtaatcc ggcaaaaaac caattccttc acctaaaatc tggcactgac
     8521 atttggtgtt gaaatctggc accagaattg actcctgccc atgcagcaac cagcccactt
     8581 ttttattaat cgtatgcgcg gtgtcctcca ccatgatatt agggtagaga cgcagctggc
     8641 tttcggcgat cggctccggc acaaaggcta atgggtgatc cggggcgatg gcaaaagccc
     8701 agcgaatcgc gccaatctcc gtgtaatcaa tgccgccgcc gtccagcagc gtgtcgggcg
     8761 cgccgatggc gatatttgcc tgattattaa taatcgcatc ccagacgccg ttatacactt
     8821 cggtcgtcac ggtaatctgg caggtaggaa actgcttttt cagcacctgt aataagcgcg
     8881 cggtgtgttt gggcgtataa agcagctgat taatacaaat gcgcacacgc gcttctatgc
     8941 cctgcgaaat agtatcgata ctgcgtttaa tggcgtgaaa atcattcagc aggtcggtag
     9001 ctttgcggaa aaaataacgc ccggactccg ttaactcaat gctgcgggta ctgcgagtga
     9061 acagcaccac atccagcccc gtctccatac gtttaatggt gtagctaatg gcggaagtgg
     9121 ttaaacccaa ctcttctgcg gctttactga agctgccaaa acgcgcagcg gtagtaaatg
     9181 ccaacagatt ctcttcggta aaaattgagt tcattaaacc attcccgcca ttatctaata
     9241 tgaatttctc caggcataac tgtagaggta tcaattaaag atgggtaatc atttttgaat
     9301 aacttttaat acccgtcgcg tttagtcgtc gcttcgctta atttttgtcc gccatattct
     9361 gaaattatcc ccacgcagtt acccgccaga aaacccagta atgtcaccgt ccagttcatc
     9421 ccgctggcaa aaaatagcgt catgccgaga aagcccccgg gaataaaaga cagtaaccag
     9481 aaacgccctt gccagaccac caccgccgag aagggtacag tcgtaattac gctcgcccag
     9541 accccggaaa tcccactcac cggggccagc caaccggcag ccagcgcggc gaaaaacgcc
     9601 cacaccatcc cggaataatt aaccgccagg cttctgataa accccgattt accgccgcct
     9661 gcggcataaa aactgcaaaa ggcgacgaag ccaatggttc ctaataattc ccatccctga
     9721 gagagcccga catgtgaaga cactaactgc caaagccctg cacaaatacc gacggtgaca
     9781 cccgttgccg tgagtccgtt catcttgttc ccctctggtt agacatggac gaaagctact
     9841 cttacatgtg cagactatag agaaataaaa cctgattgag tgaaagggta tcgggtcaaa
     9901 gaaacaaata ttgcactacc gcacacggcg aaaagattgt tgaacatcat tcaacaaaaa
     9961 ggcgaaatac ggcgaattaa atttaagcgg gttcttttat cagattatcg ttgctattct
    10021 caattcatcg aaaacagcca caaaaaccag gtgaatgtga atgactataa aaaatctacc
    10081 tgcagactat ttattggctg cgcaacaggg tgatatcgat aaagtaaaaa cctgccttgc
    10141 gctgggtgtc gatattaata cctgcgatcg tcaggggaaa acggcaatta cgctggcaag
    10201 tttatatcag caatatgctt gcgttcaggc attaattgac gccggagcga atattaataa
    10261 acaagatcat acctgtttaa atcctttttt aatgagctgt ctgaacgatg atttaacgct
    10321 actacgaatt attttaccgg ctaaacccga tcttaattgc gtaacccgtt ttggcggtgt
    10381 cggcctgacg cctgcctgtg aaaaaggcca tttaagtatt gtaaaagagc ttttggcaaa
    10441 tacggagatt aacgttaacc agactaacca tgttggctgg acaccgttgc tggaagcgat
    10501 tgtgcttaac gatggcggga ttaaacagca ggcgattgtc cagttattgc tggaacacgg
    10561 tgccagcccg catctgaccg ataaatatgg caaaacgccg ctggaactgg cgcgggaacg
    10621 gggctttgaa gagattgcgc agttgctgat cgccgcaggt gcataaaccg ggaggcttgt
    10681 tatcaacaca ccagaaaaac ggtgtgtgtg ggcgctaact gcggatgctg attttctggc
    10741 gcagcggggg caaggacagg ttgaacaggt ctttgccaga gcggtaaata tcgcactccc
    10801 ggctcgccag cagttgctga cgctgctttg tgaagagtac gacaatgcgc ctaacagttg
    10861 tcggttggca ctcactcact ttaatggtct gttccggcat ggtgataagg ttcagtttga
    10921 cgatcaaggc attacggttg gtcaacatca tcatatagag atgagtcatt gccagcgttg
    10981 gctgtcccca accttgcaaa tgaccgctct gaattttcac cttatcgcct ggcaacagtg
    11041 gcacgacatt attcatcagc acctggggga aaatgaaacc ctgtttaatt atcgcggcga
    11101 taatccgttt tatcaggcgt taaataaaga actacatatt aaacgacggg cagttattca
    11161 ggccgtaaac gataaacaaa atatcgccgt agcggtcgcc agtatgatgg gcttagggat
    11221 tggccttacg ccatcagccg atgattatct gaccggactg gtacttgttt tatttatttc
    11281 cggacatccg gcggaaaaat acaaagagga attttatctc ggtttgcaac gcggcagaaa
    11341 taataccacg ttattaagtg ccatcacgct ggaagccgca ttacaacaac gctgccggga
    11401 aaatattcat cgttttattc acaacattat ttatgacatc cctgggaacg caactcaggc
    11461 aatagaaaaa attaaacata ttggctccag ttccggctgc gacatgctgt atggcatggc
    11521 cgatggttgt gcgctgagcc aaacttacgg agggaattat gtcagttaaa atagtcatta
    11581 aaccgaatac ctattttgat tctgtctcgc tgatgtctat ctccacgcgt gcaaataaac
    11641 tcgacggcgt cgagcaggca tttgtggcga tggcgaccga aatgaacaaa ggcgtgttga
    11701 agaatttagg actgctgacg ccggagctgg agcaggcgaa aaacggcgac ctgatgattg
    11761 tcatcaatgg taaatcgggt gtggacaacg agcagttgct ggcggagatt gaagaactgt
    11821 tcaacaccaa agcgcaaagc ggctcgcacg aggcgcgtta cggcactatt gccagcgcca
    11881 aaaagcatat cccggaaagt aacctggcag tgatttcggt caacggtctg tttgccgctc
    11941 gcgaagcgcg tcaggcgctg caaaatgacc tcaacgtgat gctgttttct gataatgtct
    12001 cagttgaaga tgaactggcg ctcaaacagt tggcccatga aaaagggctg ctgatgatgg
    12061 ggccagactg cggcacggcg attatcaacg gcgcggcgct ctgctttggt aacgccgtgc
    12121 gtcgcggcaa catcggtatt gttggcgcat ctggcaccgg cagccaggaa ttgagcgtcc
    12181 gtatccatga atttggtggc ggcgtttcac aactgatcgg caccggcggg cgcgacctga
    12241 gcgagaaaat cggtggcctg atgatgctcg atgccatcgg gatgctggaa aacgatccgc
    12301 aaactgaaat cattgtgctt atctccaaac cgcctgcgcc tgcggtggcc cgcaaagtgc
    12361 tggaacgcgc acgcgcctgc cgcaagccag tggtggcctg cttcctcggt cgtggcgaaa
    12421 cgccagtgga tgagcagggg ctacagtttg ctcgcggcag caaagaggcg gcattgaaag
    12481 cggtgatgct ctccggcgtg aaacaagaac atctcgacct gcatacgctt aaccagccgt
    12541 tgattgcgga tgtgcgggcg cgtctgcaac cgcagcagaa atacattcgt ggtctgttct
    12601 gcggcggcac gctgtgtgac gaaaccctgt tcgcggtgat ggaaaaacat ggcgatgtct
    12661 acagcaatat ccagcccgat cctgaattcc gcctgcaaga tatcaaccgc agcatcaaac
    12721 acaccttcct cgactttggc gatgatgact tcaccaacgg caagccgcat ccaatgattg
    12781 acccgaccaa ccgtatcagc cgcttgatcg aagaagcgcg cgatccagaa gtggcggtga
    12841 tcgtgatgga ttttgtgctc ggatttggat cgcatgaaga tccggtcggt tccaccatcg
    12901 aggcgatcaa agaagcgaaa gcgatcgccg ctgccgaagg acgcgagttg atcattctcg
    12961 cctatgtgct gggcaccgat ctcgatacgc catcattaga acaacaaagc cagatgctgc
    13021 ttgatgccgg agtgattctg gcgagcagca gcaccaatac cggattgctg gcgcgtgaat
    13081 ttatctgcaa aggggaggaa gcctgatgag ccagtcactg tttagccaac cgttgaacgt
    13141 tattaacgtc ggtatcgcca tgtttagcga tgatctgaaa aagcagcatg tagaagtgac
    13201 ccaactcgac tggacgccgc cggggcaggg caatatgcag gtggtgcagg cactggacaa
    13261 cattgccgat tcgccgctgg cggacaaaat caccgccgct aaccagcagg cgctggagcg
    13321 cattatccag tcgcatccgg tgctgattgg ttttgatcag gcgattaacg tggtgccggg
    13381 catgacgccg aaaaccattc ttcacgccgg gccgccggtc acctgggaaa aaatgtgcgg
    13441 cgcgatgaaa ggcgcggtca ccggagcgct ggtgtttgaa ggactggcga aagatctcga
    13501 cgaggcggct gagctggcgg cttccgggga gatcaccttc tcgccgtgcc acgaacacga
    13561 ctgtgtggga tcgatggcgg gcgtcacctc ggcctcgatg tttatgcaca tcgtgaaaaa
    13621 caaaacctat ggcaacatcg cttataccaa catgagcgag cagatggcga agattttgcg
    13681 tatgggcgct aacgaccaga gcgtgatcga ccgcctcaac tggatgcgcg atgtgcaggg
    13741 acccatgctg cgcgacgcga tgaaaattat cggcgaaatc gatctgcgct taatgctggc
    13801 gcaggcgctg catatgggcg atgaatgcca taaccgcaat aacgccggga cgacgctgct
    13861 gattcaggcg ctgacgccgg ggattattca ggcaggttat tccgtcgagc aacagcgcga
    13921 agtgtttgag tttgtcgcca gcagcgacta cttctccggc ccgacgtgga tggcgatgtg
    13981 taaagcggcg atggatgcgg cgcacggcat cgaatacagc accgtggtga ccaccatggc
    14041 gcgtaacggc gtcgagttcg gcctgcgggt cagcggcctg ccggggcagt ggtttaccgg
    14101 cccggcgcag caggtcatcg gcccgatgtt tgccggttat aagccggaag attcagggct
    14161 ggatattggc gacagcgcca tcaccgaaac ctacggtatt ggcggatttg cgatggcgac
    14221 cgcgcccgcg atcgtcgcgc tggtgggggg cacggtggaa gaggctatcg atttctcccg
    14281 tcagatgcgc gaaatcactc tcggcgaaaa ccccaacgtc accattccgc tactggggtt
    14341 tatgggcgtg ccatcggcaa ttgacatcac ccgcgtgggt agcagcggca ttctgccggt
    14401 gatcaacacc gccattgccc ataaagatgc gggcgtcggc atgattggcg cgggcattgt
    14461 gcatccaccc ttcgcctgct tcgagaaagc catttttggc tggtgcgaac gttacggcgt
    14521 ctgatctgta aagtgcgcgt tgtttctgcc ggatgcggcg tgaacgcctt atccggccta
    14581 cgaatggcgc ggaaatctgt aggcctgata agcgtggcgc ttcaggcatt ggccaccaca
    14641 gccggatgcg gcgtgaacgc cttatccggc ctacgagtgg cgcaagaatc tgtaggcctg
    14701 ataagcgtag cgcatcaggc attggtcacc acagccggat gcggcgtgaa cgccttatcc
    14761 ggcctacgaa tggcacggga atctgtaggc ctgataagcg tagcgcatca ggcagtctgg
    14821 cgttggtcat aaccccatca ccctctgtcg cggacataac aacatgaaag aacttgtggt
    14881 cgttgccatt ggtggcaaca gcattatcaa agataacgcc agccagtcga ttgagcatca
    14941 ggcggaggcg gtgaaagccg tggccgatac ggtgctggaa atgctggctt ccgattacga
    15001 cattgtgctg acccatggca acgggccgca ggtcgggctg gatttacgcc gcgcggagat
    15061 tgcccacgag cgcgaagggc tgcccttaac gccgctggcg aactgtgtgg cggatacgca
    15121 aggtggcatt ggctatctga tccagcaggc gctgaacaac cgactggcgc gtcacggcga
    15181 gaaaaaagcc gtcaccgtgg tgactcaggt agaagtggat aaaaacgatc cggggtttgc
    15241 ccatcccatc aaacccattg gcgcattctt tagtgaaagc cagcgtgacg aactacaaaa
    15301 ggcaaaccct gactggcgtt ttgttgaaga tgccggacgg ggctatcgcc gcgtggtcgc
    15361 ctcgccggaa ccgaaacgta ttgtcgaagc acctgccatt aaggcgctga tccaacaagg
    15421 ctttgtggtg attggcgcgg gcggcggcgg aattccggta gtgcgtactg acgcggggga
    15481 ttaccaaagc gtggacgcgg ttatcgacaa agatctctct accgcgctac tggctcgcga
    15541 aattcacgcc gacattctgg tgatcaccac tggcgtcgaa aaagtgtgta ttcactttgg
    15601 caaaccgcag cagcaggcgc tggatcgcgt agatattgcc accatgaccc gctatatgca
    15661 ggaagggcat ttcccacctg gcagcatgtt gccaaaaatc atcgccagcc tgacgttcct
    15721 ggaacaaggc ggcaaagaag tgattatcac cacgccggaa tgcctgcctg cggcgctacg
    15781 cggcgaaacg ggcacccata ttattaaaac ctaaggacgt aagatgaaag aaaacaatag
    15841 ccgccgtgaa tttctgagcc agagcggtaa gatggtcacc gccgccgcgc tgtttggtcc
    15901 ctcagtgccg ctcgcccatg cgggggtagc tggcacccta aactgcgaag cgaacaacac
    15961 catgaaaatc actgacccgt attactatct cgataacgtg ctgctggaaa ccggttttga
    16021 ctacgaaaat ggcgtggcgg tacagacccg cacggcgcgc cagaccgtgg agattcagga
    16081 cggtaaaatt gttgccctgc gcgagaacaa gcagcatccg gacgccacgc tgccgcacta
    16141 tgacgctggc ggtaagctga tgctgcccac cacccgcgac atgcatattc atctcgacaa
    16201 aaccttctac ggcgggccgt ggcgctcgct caatcgtccg gcaggcacca ctatccagga
    16261 catgatcaaa ctcgagcaga aaatgctgcc ggaactgcaa ccgtacacgc aggaacgggc
    16321 ggaaaaactg atcgatttat tgcagtcgaa aggcaccacc attgcccgca gccactgcaa
    16381 tatcgaaccg gtttccggcc tgaaaaatct gcaaaatttg caggcggtgc tggcgcgacg
    16441 tcaggcgggc ttcgagtgtg aaattgtcgc cttcccgcag cacggtttgc tgctgtcgaa
    16501 atcggaagcc ttaatgcgcg aagcgatgca ggcgggggcg cattacgtcg gcgggctgga
    16561 cccgaccagt gttgatggcg cgatggaaaa atccctcgac accatgttcc agattgcgct
    16621 ggactacgac aaaggcgtcg atattcacct gcacgaaacc actccgtcgg gcgtggcagc
    16681 catcaattat atggttgaaa cggtagagaa aacgccacag ctgaagggca agctgaccat
    16741 cagtcacgcc tttgcgttgg caacgctcaa cgaacaacag gtagatgaac tggcgcaccg
    16801 gatggcggcg cagcaaattt ctatcgcctc gacggtgccg attggcacgc tgcatatgcc
    16861 gctcaaacag ttgcacgaca aaggcgtaaa agtcatgacc ggcaccgaca gcgttatcga
    16921 ccactggtct ccctacggcc tgggcgacat gctggaaaaa gccaatctct acgcgcagct
    16981 ctatattcgt cctaacgaac agaatttgtc ccgttcgctg tttttagcca ctggcgatgt
    17041 attgccgctc aacgaaaaag gcgagcgcgt gtggcccaaa gcgcaggatg acgccagctt
    17101 tgtgctggtg gacgcctcct gttccgccga ggcggtggcg cgtatctcgc cgagaaccgc
    17161 aacgttccat aaagggcaac tggtgtgggg gagtgtggca ggttgatacg aacttgatat
    17221 atctgcaacg gtgaatcgta agagatatta agccccaggg agtggggcga cactggtttg
    17281 ttaatgaatg atcctgaact ttataatctc caggcataat ttccaggtga ttatttgaga
    17341 gaaatacaca aaagtgagtt ttaacaaagg tatttatcag gccataaatg tgacctgctt
    17401 cggataaaaa agattaaaca tagaaaaacc aatgatactc ttgctgatgg ttatcaacag
    17461 taattaagat gtgatttgtt tttccgtaat cgcgaaaata ttctcatgac cataaaagaa
    17521 aaattctcac aaaaatatcc ccatgcttcc ttttgtactt ttggcgattc agcagcgttg
    17581 gccgatcacc tcgcaacgtt gattgcgact ggtgttaaaa cagcttcctg tggttcgctg
    17641 gcgggatgta ttgaagacaa cgcatttccg atgattgggg agtataagat tgtagaaaat
    17701 agccgtggtg agcctgtctg tgttatcaga gtgattggcc tgcatttact gcgcttttct
    17761 gatgtcaccg cggagcttgc ccgtaaggaa ggtgaaggtg acctcagtct tgaatattgg
    17821 cgtaatgaac accgtcgttt tttccaggca gaaggatgct attcacctga aatggacgtt
    17881 atctttgaag aatatgccct tattgacgtt gtgtagataa ctatttttct acgcgaaata
    17941 agctcacaaa cataaagtat cgctaatttt gtttattttg ctatttttta cgtgatgatt
    18001 gtcacactta ttgttactgc ccgttttgtc catatttata accatatttt taatagctgc
    18061 ctgaaatagg gctgtttatt ctgcataaaa atattcgaaa attcttattc tctccagcgc
    18121 gctaatttgg gcgaacactt cctgactacc ctgcaatgag gctgaaatga tgaataaacg
    18181 ttttgttatc aatatggtgt cttcgctgtt attaggcgct gcgctaattt ctgcgccgct
    18241 ccaggcagcg gaaaaagtcg tggtgaatat ctctaaagtc gatggtatgc cgtggtttaa
    18301 tcgcatgggc gaaggtgtgg ttgaggcggg taaagccttt ggtgttaatg cgtcacaggt
    18361 ggggccttca agtactgatg ctccgcaaca ggttaaaatc attgaggatt taatcgcccg
    18421 taaggttaat gcaattacaa ttgtaccaaa cgatgccaac gtgctggaac cggtattcaa
    18481 aaaagcgcga gatgccggaa ttgtggttct gaccaacgaa tctcccggtc agccaagtgc
    18541 taactgggat atcgaaatca ttgataacga aaaatttgcc gctgaatatg ttgagcatat
    18601 ggcgaaacgc atggggggca agggcggcta tgtgatttac gtggggagtt tgaccgtgcc
    18661 gcagcataac ttatgggctg acttgctggt gaaataccag aaagagcatt acccggatat
    18721 gcatgaagtt acccgccgca tgccggttgc cgagagtgtg gatgactcac gtcgtaccac
    18781 actggacctg atgaaaacct atcctgactt gaaagcggtt gtctcctttg gttcaaacgg
    18841 tccgattggc gcgggacgtg cagtgaaaga gaaacgggcg aaaaataaag tcgcagtcta
    18901 tggcatgatg atcccttcac aggcggcatc actgattaaa agtggtgata tcaccgaagg
    18961 gattacttac gatccggcct ctgcaggtta cgcactcgcg gccgtcgcca gcacgttgct
    19021 gaagggtgaa gagatcaaac ctggattaga aatggagaat ctcggtaagg ccgacgtaga
    19081 tatggataag cgaattatcc gtttccataa ggtattactg gtcaacaaag acaatatcga
    19141 ctcactttat taatccattt cttcggggcg aaagccccga ttcttattgc caggggatgc
    19201 tatggaaacc ttcctttccc ttcgtcatat caacaaaacg tttcatgcca cgcgagcgct
    19261 gcgcgatgtt tctctcgact ttatgtcggg tgaggtgcac tgccttgccg ggcaaaacgg
    19321 ctgtggtaaa tcgacgttga ttaaaattat gtccggtgtt tatcgcccgg atgagggggc
    19381 ggaaattacg cttggtggga aaaactggtc aaagctaaca cccgccgctt cggtggcgca
    19441 ggggattcag gtgatttatc aggacctctc tttatttcct aacctgagtg tctgggagaa
    19501 tatcgccgtg aatcactatc accacggtct gtttgttaac cgacgtcgtc tgcgtgaggt
    19561 ggcgcaggcg gcaatgacca gtattaacgt cacattgcca ctggatacgc tcgtttctga
    19621 gctttcaatt gcccgctgtc agctggtggc aatttgccgt gcactggcgc aggacgcacg
    19681 gttgatcgtg atggatgaac caaccgcttc gctgacccat caggaagtgc aagggttatt
    19741 acaggtggtg catcaattgc gggaacgcgg gatctgcgtg gtcttcgtca gtcatcgtct
    19801 ggaagaggtc atggaagttt ccgaccgtat ttcagtgttg aaagatggtg agctggtcgg
    19861 gacctttccg gccgcagaga tgaccacaaa acaactcagt tttctgatga ccggccagga
    19921 gtttgaatat caggagcgag agttgtggca gggaaaatcc agcaccccgg tgctggaggt
    19981 gcgtaattta agtcgtcatg gggaatatct aaatattaac ttgcgggtgg aagcgggcga
    20041 agtggtatcg attgttggcc tgcttggtgc ggggcgcacg gaattatgtc tgagcctgtt
    20101 tggtatgacc cgaccggatg ctggcgagat cctcatcaac ggccagctgg ttacactgca
    20161 tagtaaccag gacgccatcc gccacggtat tggttatgtt tcggaagatc gcatgtcgcg
    20221 cggtttggtg atggcgcaat ctattgaaga caacatcatt agcactgttt ttcacaaggt
    20281 aaaagatcgc ttcggctttc tgagtgaagc taaagtgcgt gacctggtgg acaggctaat
    20341 caaggcattg accatcaaag tctctgatcc tcatctcgcg gtgaatacgc tttctggtgg
    20401 taacgcgcag cgagtatcga tcgccaaatg gctggcgatt gggccacgac tgttgattct
    20461 cgatagcccg acagtcggtg ttgatatcgc caacaaagca ggaatatacg gcattatcag
    20521 cgatctggcg gctcatggca ttgcggtatt gatgatctgc gatgaaattg aagaagcgtg
    20581 gtatcaaagc caccgtattt tggtaatgca aaaaggtcag atcacccata gcttcctgcc
    20641 tgacagcagt tctcaggcca ggattgcgga ggtggtcaat ggctgaactg aaaaaacgcc
    20701 atgaattctg gctggcatta ctgattgtgg tgctgtttgt tggccttgcc tggcgcagcg
    20761 acgagtttct gacattcggt aatttgtacg atctcgccaa taactatgcc atgttgacta
    20821 ttctcgcctg tggtttgttt gtggtgttga tttccggtgg aattgatatt tcgtttccag
    20881 caatgaccat cattgcgcaa tacggcatgg tgctgttgct acaaaaaatt ggtggtaact
    20941 ttgctgtcgc gtttgcactg gcgggcggca tcggcatttt acttggctta attaacgcct
    21001 tactggttaa tcgcctacgg gtgccttcta tcatcatcac tatctcgacg ctgaatattt
    21061 tttatggcct gctgttatgg ttgagtaaag gtgtgtggct gtacgacttt ccgccgtggt
    21121 ttgagcaggg ggtaatgttg ttcaagtaca ccgatgctga tggctatgac tatggccttg
    21181 gtctgccgct gatcgccatg attacggtgg tgctgctgac agcgtttatc atgaatttca
    21241 ccagtgtagg gcgcaaaatt tatgcccttg gcgggaaccg tgaatccgcc agtcgcatcg
    21301 gctttagcgt gctgaaactg caacttttcg tctacggcta tatgggattg atgtctggtg
    21361 ctgcgggtgt agtgcagtcg tggacggtga tgactgtcgc ccccgattct cttctgggtt
    21421 atgagctgac agtactggct gcggtggtgc ttggcggcac tagtttgctc ggcgggcgcg
    21481 gcacgttaac cggtactttg ctcggcgtgg tgttgttggc agtgatgcaa aacgggctaa
    21541 atttattggg agtctcgtct tactggcaaa cattgatcac cggcatcatc atcgttgcca
    21601 gcattagcgc cacggcgtgg agtcagcatc agaaccggag tctgctatga aaaaatcgtg
    21661 gcgtaataac gttgagtttt acttgatcgg cctgctggtg ctaactgttg ccgccttcag
    21721 cattactatg ccggagattt tctggtcgat aagtaatttc cagtccgtcg cttcgcaaat
    21781 gcccgtgctg ggcattctgg cgttggctat ggcggtgacc atgctttgcg gtggcattaa
    21841 cttgtcaatt attgccactg ctaacgcctg ttcactggtg atggcgtggg tggcgactca
    21901 gtatccaccg ggtattgcta cggttgtcgc tacgctgctg gcgggcgctg gcgcggcggt
    21961 gattatcggt ttatgtaacg gcgtattgat tgctggtatt cgagtgtcgc caattcttgc
    22021 aacacttggg atgatgacgt tgctcaaagg ggtaaatatt ctggtgacag gcggtagcgc
    22081 cattgccaat tatccctcat gggtgctgtg gttgaatcat gcacagtggt tcggtattcc
    22141 gctaccgatg tggttgttta ccgcagtggc gctggggctg tggatattgc tggagaaaac
    22201 gccgctcggt aaagccattt atctgattgg ttctaacgaa cgagcaacac tttactccgg
    22261 tattaacact cgtcgggtgc tgatatgggt gtatgtcatt tccgccttgc tttgtgcggt
    22321 agcggcattt ttgatgatgt cgaaactcaa ctcggctaag gcctcttatg gcgaatcgta
    22381 cttgctggtg tcaatcctcg cggcagtact cggcggggtt aatccggacg gcggcagcgg
    22441 gcggataatt ggtatggtgc tggcgctgtt tcttctgcaa attattgaaa gcggcttcaa
    22501 tattctcggt ataagcccct acctgacgat ggccttatgg ggaacgttgc tgctctgttt
    22561 cattcaggct cgcggcatgt tggggctgga tcgggtggtt taaatattgg ctatattcaa
    22621 tggacgcgtt ttgccgcgat gacatatcag gcatcgccaa atacacatag ctaatcagga
    22681 gtaaacacaa tgaagatcaa agctgttggt gcatattccg ctaaacaacc gctggaaccg
    22741 atggatatca cccggcgtga accgggaccg catgatgtca aaatcgaaat cgcttactgt
    22801 ggcgtctgcc attccgatat ccaccaggtc cgttccgagt gggcggggac ggtttacccc
    22861 tgtgtgccgg gtcatgaaat tgtggggcgt gtggtagccg ttggtgatca ggtagaaaaa
    22921 catgcgccgg gcgatctggt cggtgtcggc tgcattgtcg acagttgtaa acattgcgaa
    22981 gagtgtgaag acgggctgga aaactactgt gatcacatga ccggcaccta taactcgccg
    23041 acgccggacg aaccgggcca tactctgggc ggctactcac aacagatcgt cgttcatgag
    23101 cgatatgttc tgcgtattcg tcacccgcaa gagcagctgg cggcggtggc acctttgttg
    23161 tgtgcaggga tcaccacgta ttcgccgcta cgtcactggc aggccgggcc gggaaaaaaa
    23221 gtgggcgtgg tcggcatcgg cggtctggga catatgggga ttaagctggc ccacgcgatg
    23281 ggggcgcatg tggtggcatt taccacttct gaggcaaaac gcgaagcggc aaaagccctg
    23341 ggggccgatg aagttgttaa ctcacgcaat gccgatgaga tggcggctca tctcaagagt
    23401 ttcgatttca ttttgaatac agtagctgcg ccacataatc tcgacgattt taccaccttg
    23461 ctgaagcgtg atggcaccat gacgctggtt ggtgcgcctg cgacaccgca taaatcaccg
    23521 gaagttttca acctgatcat gaaacgccgt gcgatagccg gctctatgat tggcggcatt
    23581 ccagaaacac aggagatgct cgatttttgc gccgaacatg acatcgtggc tgatatagag
    23641 atgattcggg ccgatcaaat taatgaagcc tatgagcgaa tgctgcgagg tgatgtgaaa
    23701 tatcgttttg ttatcgataa tcgcacacta acagactgaa acaattaata aatatcctgt
    23761 ggtttaacat attaacttcg ctctcca
//
LOCUS       DAZKHK010000086        22679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKHK010000086 DAZKHK010000000
VERSION     DAZKHK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.086"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..3224
                     /locus_tag="ACHOS5_003331"
     CDS             <1..3224
                     /locus_tag="ACHOS5_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9778099.1"
                     /translation="LTDAVNGLGDDSLLWNKTAGAFSAAHGTDATSKITNVKAGDLTA
                     GSTDAVNGSQLKTTNDNVSTNTTNIATNTTNITNLTDAVNGLGDDSLLWNKTAGAFSA
                     AHGTDATSKITNVKAGDLTAGSTDAVNGSQLKTTNDNVTTNTTNIATNTTNITNLTDS
                     VGDLKDDSLLWNKAAGAFSAAHGTEATSKITNLLAGKISSNSTDAINGSQLYGVADSF
                     TSYLGGGADISDTGVLSGPTYTIGGTDYTNVGDALAAINTSFSTSLGDALLWDATAGK
                     FSAKHGINNAPSVITDVANGAVSSTSSDAINGSQLYGVSDYIADALGGNAVVNTDGSI
                     TTPTYAIAGGSYNNVGDALEAIDTTLDDALLWDTTANGGNGAFSAAHGKDKTASVITN
                     VANGAVSATSRDAINGSQLYSTNKYIADALGGDAEVNADGSITAPTYTIANTDYNNVG
                     EALDALDNNALLWDATANNGEGAYSASHDGKASNITNVADGNIGEDSTDAINGSQLNA
                     TNMMIQQNSEIINQLAGDTSKNYIGDNGAGINYVRTNDSGLAFNDASAAGIGSTAVGY
                     NTVAKGDSSVAIGQNSYSKVETSIALGSESVSSRVIVKGSRNTSVSEEGVVIGYDTTD
                     GELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAE
                     DSLAVGEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGN
                     GSQTTRGAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKV
                     TDAQVSQNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQG
                     KDSVAIGSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGVNATDAVNV
                     SQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQ
                     SVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTY
                     NGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3636..5291
                     /gene="lldP"
                     /locus_tag="ACHOS5_003332"
     CDS             3636..5291
                     /gene="lldP"
                     /locus_tag="ACHOS5_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9778100.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            5291..6067
                     /gene="lldR"
                     /locus_tag="ACHOS5_003333"
     CDS             5291..6067
                     /gene="lldR"
                     /locus_tag="ACHOS5_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP9778101.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            6064..7254
                     /gene="lldD"
                     /locus_tag="ACHOS5_003334"
     CDS             6064..7254
                     /gene="lldD"
                     /locus_tag="ACHOS5_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP9778102.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQVLGKELPAALAPMAK
                     GNAA"
     gene            7302..7775
                     /gene="trmL"
                     /locus_tag="ACHOS5_003335"
     CDS             7302..7775
                     /gene="trmL"
                     /locus_tag="ACHOS5_003335"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9778103.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7830..8651)
                     /gene="cysE"
                     /locus_tag="ACHOS5_003336"
     CDS             complement(7830..8651)
                     /gene="cysE"
                     /locus_tag="ACHOS5_003336"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9778104.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8731..9750)
                     /gene="gpsA"
                     /locus_tag="ACHOS5_003337"
     CDS             complement(8731..9750)
                     /gene="gpsA"
                     /locus_tag="ACHOS5_003337"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9778105.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9750..10217)
                     /gene="secB"
                     /locus_tag="ACHOS5_003338"
     CDS             complement(9750..10217)
                     /gene="secB"
                     /locus_tag="ACHOS5_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP9778106.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10280..10531)
                     /gene="grxC"
                     /locus_tag="ACHOS5_003339"
     CDS             complement(10280..10531)
                     /gene="grxC"
                     /locus_tag="ACHOS5_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP9778107.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10672..11103)
                     /locus_tag="ACHOS5_003340"
     CDS             complement(10672..11103)
                     /locus_tag="ACHOS5_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9778108.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11348..12892
                     /gene="gpmM"
                     /locus_tag="ACHOS5_003341"
                     /gene_synonym="pgmI"
     CDS             11348..12892
                     /gene="gpmM"
                     /locus_tag="ACHOS5_003341"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP9778109.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFTALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12926..14185
                     /gene="envC"
                     /locus_tag="ACHOS5_003342"
     CDS             12926..14185
                     /gene="envC"
                     /locus_tag="ACHOS5_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP9778110.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            14189..15148
                     /locus_tag="ACHOS5_003343"
     CDS             14189..15148
                     /locus_tag="ACHOS5_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP9778111.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPENTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(15135..16169)
                     /gene="waaH"
                     /locus_tag="ACHOS5_003344"
     CDS             complement(15135..16169)
                     /gene="waaH"
                     /locus_tag="ACHOS5_003344"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP9778112.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16408..17433)
                     /gene="tdh"
                     /locus_tag="ACHOS5_003345"
     CDS             complement(16408..17433)
                     /gene="tdh"
                     /locus_tag="ACHOS5_003345"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9778113.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMTELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17443..18639)
                     /gene="kbl"
                     /locus_tag="ACHOS5_003346"
     CDS             complement(17443..18639)
                     /gene="kbl"
                     /locus_tag="ACHOS5_003346"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9778114.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18853..19785
                     /gene="rfaD"
                     /locus_tag="ACHOS5_003347"
     CDS             18853..19785
                     /gene="rfaD"
                     /locus_tag="ACHOS5_003347"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP9778115.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19795..20841
                     /gene="rfaF"
                     /locus_tag="ACHOS5_003348"
     CDS             19795..20841
                     /gene="rfaF"
                     /locus_tag="ACHOS5_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP9778116.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20845..21804
                     /gene="rfaC"
                     /locus_tag="ACHOS5_003349"
     CDS             20845..21804
                     /gene="rfaC"
                     /locus_tag="ACHOS5_003349"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP9778117.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPLKSLNNLEAAAVYKEITLI"
     gene            complement(21852..>22679)
                     /locus_tag="ACHOS5_003350"
     CDS             complement(21852..>22679)
                     /locus_tag="ACHOS5_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFP9778118.1"
                     /translation="QGIYLHADRIPLSAMNGNIGQMGAATIAAYVITFTTLYSSIVFI
                     KMQSKYKWFLFYANFALSFAAVMMTGTRAAIFTFPLMIMVILFLQHRDQKVFLFKGLS
                     GVFILLLACGLIFNKEIDRRINSLKADVISYATKNNSQSSVGARFAMVNAGIKGSPDG
                     FNWQSLEQRAEKIKALSAENNIYSGALLFLDVHMHNEIVEALSTKGKIGLLVLIMFYV
                     AMIYYCIREKKYILLVFPASIMLFGISDVITHAKPIPASWIVCLFLSISFLSKKEPQ"
BASE COUNT         5650 a         5942 c         5832 g         5255 t
ORIGIN      
        1 acctgactga cgctgttaac ggtctcggtg acgactccct gctgtggaac aaaacagctg
       61 gcgcattcag cgccgctcac ggcactgacg ccaccagcaa gatcaccaat gtcaaagccg
      121 gtgacctgac agctggcagc actgacgccg ttaacggctc ccagctcaaa accaccaacg
      181 ataacgtgtc gaccaacacc accaacatcg ccactaacac caccaacatc accaacctga
      241 ctgacgctgt taacggtctc ggtgacgact ccctgctgtg gaacaaaaca gctggcgcat
      301 tcagcgctgc tcacggcact gacgccacca gcaagatcac caatgtcaaa gccggtgacc
      361 tgacagctgg cagcactgat gccgttaacg gctctcagct caaaaccacc aacgacaacg
      421 tgacgaccaa caccaccaac atcgccacta acaccaccaa catcaccaac ctgacggatt
      481 ccgttggcga ccttaaggac gattctctgc tgtggaacaa agcggctggc gcattcagcg
      541 ccgcgcacgg taccgaagct accagcaaga tcaccaactt actggctggc aagatatctt
      601 ctaacagcac tgatgccatt aatggctcac aactttatgg cgtagcggat tcatttacgt
      661 catatcttgg tggtggtgct gatatcagcg atacgggtgt attaagtggg ccaacctaca
      721 ctattggtgg tactgactac actaacgtcg gtgatgctct ggcagccatt aacacatcat
      781 ttagcacatc actcggcgac gccctacttt gggatgcaac cgcaggcaaa ttcagcgcca
      841 aacacggcat taataatgct cccagtgtaa tcactgatgt tgcaaacggt gcagtctcgt
      901 ccaccagcag cgacgccatt aacggttcac aactttatgg tgttagtgac tatattgccg
      961 atgctctggg cgggaatgct gtggtgaaca ctgacggcag tatcactaca ccaacttatg
     1021 ccatcgctgg cggcagttac aacaacgtcg gtgacgcgct ggaagcgatc gataccacgc
     1081 tggatgatgc tctgctgtgg gatacaacag ccaatggcgg taacggtgca tttagcgccg
     1141 ctcacggaaa agataaaact gccagtgtaa tcactaacgt cgctaacggt gcagtctctg
     1201 ccaccagtag agacgccatt aatggctcgc agctctatag caccaataag tacattgctg
     1261 atgcactggg tggtgatgca gaagtcaacg ctgacggttc tatcactgcg ccgacttaca
     1321 ccattgcaaa taccgattac aacaacgtcg gtgaagccct ggatgcgctc gataataacg
     1381 cgctgctgtg ggatgcgacc gccaataacg gcgaaggtgc ttacagcgcc agtcatgacg
     1441 gcaaggccag caatatcaca aacgttgctg atggtaacat tggtgaagat agcaccgacg
     1501 ctatcaacgg ttctcagttg aatgcaacca acatgatgat ccagcagaac agcgaaatca
     1561 tcaatcagct tgccggtgac accagtaaaa actacatcgg agataacggt gcaggtatta
     1621 actatgtacg taccaacgac agcggcttag cgttcaacga tgccagcgca gcaggtattg
     1681 gctctaccgc tgtggggtat aacactgttg ccaaaggcga tagcagcgtg gccatcggtc
     1741 agaacagcta cagcaaagtt gagacgagta ttgcgctggg tagcgaatcc gtctccagcc
     1801 gtgtgattgt taaaggttct cgtaacacca gcgtatcaga agaaggagtt gtgattggtt
     1861 atgacaccac ggatggcgaa ctgcttggcg cgttgtcaat tggtgatgat ggtaaatatc
     1921 gtcaaatcat caacgtcgcg gatggttctg aagcccatga cgctgttacg gttcgccagt
     1981 tgcagaacgc cattggtgca gtcgcaacca caccaaccaa atactatcac gccaactcaa
     2041 cggctgaaga ctcactggca gtcggtgaag actcgctggc aatgggcgcg aaaaccatcg
     2101 ttaatggtaa tgcgggtatt ggtatcggcc tgaacacgct ggttctggct gatgcgatca
     2161 acggtattgc tatcggttct aacgcacgcg caaatcatgc cgacagcatt gcaatgggta
     2221 atggttctca gactacccgt ggtgcgcaga ccaactacac tgcctacaac atggatgcac
     2281 cgcagaactc tgtgggtgaa ttctccgtag gtagcgaaga cggtcaacgt cagatcacca
     2341 acgtcgcagc aggttcggcg gataccgatg cggttaacgt gggtcagttg aaagtaacgg
     2401 acgcgcaggt ttcccagaat acccagagca ttactaacct gaacactcag gtcactaatc
     2461 tggatactcg cgtgaccaat atcgaaaatg gcattggcga tattgtaacc accggtagca
     2521 ctaagtactt caagaccaac accgatggcg cagacgccaa cgcgcagggt aaagacagtg
     2581 ttgcgattgg ttctggttcc attgctgccg ctgataacag cgtcgcgctg ggcacgggtt
     2641 ccgtagcaga cgaagaaaat acgatctctg tgggttcttc taccaaccag cgccgtatca
     2701 ccaacgttgc tgccggtgtt aatgccaccg atgcggttaa cgtttcgcaa ctgaagtctt
     2761 ctgaagcagg cggcgttcgc tacgacacca aagctgatgg ctctatcgac tacagcaaca
     2821 tcactctcgg tggtggtaac ggcggtacga ctcgcatcag caacgtttct gctggcgtga
     2881 ataacaacga cgcggtgaac tatgcgcagt tgaagcaaag tgtgcaggaa acgaagcaat
     2941 acaccgatca gcgcatggtt gagatggata acaaactgtc caaaactgaa agcaagctga
     3001 gtggtggtat cgcttctgca atggcaatga ccggtctgcc gcaggcttac acgccgggag
     3061 ccagcatggc ctctattggt ggtggtactt acaacggtga atcggctgtt gctttaggtg
     3121 tgtcgatggt gagcgccaat ggtcgttggg tctacaaatt acaaggtagt accaatagcc
     3181 agggtgaata ctccgccgca ctcggtgccg gtattcagtg gtaatgatcc attaacgaat
     3241 gggtaaacgc cacattgcct gatgcgctac gcttatcagg cctacggggt gattcagcac
     3301 taccctgtag gccggataag gcgttcacgc cgcatccggc aagataaggc gcaccagatc
     3361 aacaatctga gggcaattct ctgatgggaa ttgccctttt ctttattacc tctctcccag
     3421 aattggccct accaattctt cgcttatctg acctctggtt cacaattccc caattaaaac
     3481 tcacatcaat gttgccaaca cataacattt agttaaccat tcattgtcat tacccctaca
     3541 caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag caacagactc
     3601 attacacggc gtgtgtggac tgcaggagac ctgcaatgaa tctctggcaa caaaactacg
     3661 atcccgccgg gaatatctgg ctttccagcc tgatagcatc gcttcccatc ctgtttttct
     3721 tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg acggtggcaa
     3781 tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg ctggcctcgg
     3841 tggtttatgg cttcttctac gggttgtggc ccatcgcgtg gatcattatt gcagcggtgt
     3901 tcgtctataa gatctcggtg aaaaccgggc agtttgacat catccgctcg tctattcttt
     3961 cgataacccc tgaccagcgc ctgcaaatgc tgatcgtcgg tttctgtttc ggcgctttcc
     4021 ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg ctggtcggtc
     4081 tgggctttaa accactgtac gccgccgggc tgtgcctgat tgttaacacc gcgccagtgg
     4141 catttggtgc gatgggtatt ccgattctgg tcgccgggca ggtaacaggc atcgacagct
     4201 ttgagattgg tcagatggtg gggcgtcagc tgccgtttat gaccattatc gtgctgttct
     4261 ggatcatggc gattatggac ggctggcgcg gtatcaaaga gacgtggcct gcggtcgtgg
     4321 ttgcgggcgg gtcgtttgcc attgctcaat acctcagctc taactttatt gggccggaac
     4381 tgccagacat tatctcttcg ctggtatcgc tgctctgtct gacactgttc ctcaaacgct
     4441 ggcaaccagt gcgcgtattc cgcttcggtg acttaggggc gtcacaggtt gatatgacgc
     4501 tggctcacac aggttacacg gcaggtcagg tgctgcgcgc ctggacaccg ttcctgttcc
     4561 tgaccgccac cgtaacgctg tggagtatcc cgccgtttaa agccctgttc gcttcaggcg
     4621 gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa ctggttgccc
     4681 gtatgccgcc agtggtcagc gaagcgacag cgtatgccgc cgtgtttaag tttgactggt
     4741 tctctgccac cggtaccgcc attctgtttg ctgccttgct ctcgattgtc tggctgaaga
     4801 tgaaaccatc tgacgctatc agcaccttcg gcagcacgct gaaagaactg gctctgccta
     4861 tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc ggtttgtcat
     4921 caacactggc gctggcactg gcgcacaccg gtcatgcatt caccttcttc tcgccgttcc
     4981 tcggctggct tggggtattc ctgaccgggt cggatacgtc atctaatgct ttgtttgccg
     5041 ctttgcaggc taccgccgca caacaaattg gcgtttctga cctgttgctg gttgcagcca
     5101 acaccaccgg tggtgtcacc ggtaagatga tctccccgca atctatcgct atcgcctgtg
     5161 cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc aaacacagcc
     5221 tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcctatgtc ttaacgtgga
     5281 tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg atcgtgtgcg
     5341 ggcgctgatt gatgaaaaaa acctggaagc gggtatgaag ttgcccgctg aacgtcaact
     5401 ggcgatgcaa ctcggcgtgt cgagaaattc actgcgcgag gcgctggcga aactggtaag
     5461 cgaaggtgtg ctgctcagtc gacgtggcgg cgggacgttt attcgctggc gtcatgacac
     5521 atggtcggag caaaacatcg tccagccgct gaaaacgctg atggccgatg atccggatta
     5581 cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat ggcatgcagc
     5641 aatgcgtgcc acgcctggcg aaaaagaaaa gattcagctt tgttttgaag caacgctcag
     5701 cgaagacccg gatctcgcct cacaagcgga cgttcgtttt catctggcga ttgccgaagc
     5761 ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc tgcaatcctc
     5821 tgtgaagcat agccgccagc ggatgtacct ggtacccccg gttttttcac agctgaccga
     5881 acaacatcag gcggtcattg acgccatttt tgccggtgat gctgacgggg cgcgtaaagc
     5941 aatgatggcg caccttagct ttgttcacac caccatgaaa cgattcgatg aagatcaggc
     6001 tcgccatgca cgcattaccc gcctgcctgg tgaccataat gagcattcga gggagaaaaa
     6061 cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat tctgccgccg
     6121 ttcctgttcc actatatgga tgggggggca tattctgaat acacgctgcg ccgcaacgtg
     6181 gaagatttgt cagaagtggc gctgcgccag cgtattctga aaaacatgtc tgacttaagc
     6241 ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tggcgctagg tccggtaggt
     6301 ttgtgtggca tgtatgcgcg acgcggcgaa gttcaggctg ccaaagcagc agatgcgcat
     6361 ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt ggctccggct
     6421 atcaaacgtc cgatgtggtt ccagctttat gtgctgcgcg atcgcggctt tatgcgtaac
     6481 gccctggagc gagcaaaagc cgcgggttgt tcgacgctgg ttttcaccgt ggatatgcca
     6541 acgccgggag cgcgttatcg tgatgcgcat tctgggatga gcggcccgaa cgcggcaatg
     6601 cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg cctgaacggt
     6661 cgtccgcatg atttaggtaa tatctcggct tacctcggca aaccaaccgg actggaagat
     6721 tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga ccttgagtgg
     6781 atccgcgact tctgggatgg cccgatggta atcaaaggga tacttgatcc ggaagatgcg
     6841 cgggatgcag tacgctttgg cgctgacgga attgtggttt ctaaccacgg cggtcgtcag
     6901 ttggacggcg tgctctcttc tgcacgtgcc ctgcctgcta ttgcagatgc cgtgaaaggt
     6961 gatatcgcca ttctggcaga tagcgggatt cgtaacgggc tggacgttgt gcgtatgatc
     7021 gcgctcggtg ctgataccgt actgctgggc cgcgctttcc tgtatgcgct ggcaacagcg
     7081 ggccaggcgg gtgtagctaa tctgctaaat ctgatcgaaa aagagatgaa agtggcgatg
     7141 acgctgactg gcgcgaaatc gattagcgaa attacgcaag attcgctggt gcaggtgctg
     7201 ggtaaagagt tgccggcggc attggctccg atggcgaaag ggaatgcagc ttaagggtta
     7261 gacgaatatc tgctatcctg cccccgcact aagggggcag tatgctaaac atcgtacttt
     7321 acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct aataccggct
     7381 ttcgtctgca tatcatcgaa ccgatgggat ttgcctggga cgataagcgc ctgcgccgcg
     7441 cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat cgcgcgtttc
     7501 tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg cctgctcaca
     7561 gcgccgtaag ctatcaggat ggcgactatc taatgtttgg cccggaaacg cgcggcctgc
     7621 cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgtattcca atggtgccgg
     7681 acagccgcag catgaacctg tccaatgcgg tgtcggtggt ggtgtatgaa gcctggcgcc
     7741 agctggggta tccgggagcg gtgttgagag attaagatgc ggtaggccgg ataaggcgtt
     7801 cacgccgcat ccggcacgat cacagaatgt cagatcccat caccatactc aaacgtatgg
     7861 ttaatgccgt tgaaatgctg atccatatcc attgacggtt tatcgctatc cggcttgccg
     7921 acaatgcgtg ccggaacgcc agcggcagta gtgtgcggcg gcaccggttg cagcaccacg
     7981 gaacctgcgc caatcttcgc gccgcgccca acttcaatat tgccgagaat tttcgcgccc
     8041 gcgccaatca tcacgccttc acgaattttc gggtgacggt caccaccaga tttacccgta
     8101 ccgccaagcg tcacagattg cagaatcgat acgtcgtttt caatcaccgc cgtttcacca
     8161 acgacgatgc ctgtcgcgtg gtcgagcatg ataccgcgac caatttttgc tgccgggtgt
     8221 atatcaacct ggaacgttac agagacctgg ttttgtagaa aaatagccag cgcgcgacgg
     8281 ccctgattcc acaaccagtg accgatgcga tacgcctgca aagcatgaaa acccttcaga
     8341 tatagcaacg gggttgagta tttatccacc gccgggtcgc gggtacgcac tgcctgaata
     8401 tcacaggccg cagaagcgat catttccggg tcggcggcgt aggcttcttc caccacttca
     8461 cggatagcaa tagccggcat aatgggcgat gacagcttgt tcgccagcat gtagctcagt
     8521 gcactgccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc cagcattggc
     8581 tcacagtccg ccagcgttct ggcttcagct ttaatattgt tccagacaat ttccagttct
     8641 tcacacgaca ttgcttactc cacacgataa gataatgacc gacccgttct gcgcgggccg
     8701 ggtcatagtg gtaacaaagg ttccctgggt ttagtggctg ctgcgctcgt ccttgcgtgc
     8761 acgacctagt aaagtcaatg ctgcctcgcg cgcgtttttt ccgcaatata atacttgata
     8821 aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc ggacttcttt
     8881 cgtattgcgg tagccttcca ccacctgacc aattttctcc tgcgcgcttt gtacatccat
     8941 accctgaccg agcatcatgc caaaacggcg gttacgtgac tggttatcag tacaggtaag
     9001 caccagatcg cccagccccg ccatgcccat aaaggtggca ggatcggcac ccagcgccgc
     9061 gccaagacgc gacatttcag ccagtccacg ggtgatcagc gccgtacgcg cattcgcgcc
     9121 aaaaccgata ccgtcggaca tccccgcgcc aatggcaatg acgtttttca ccgcaccgcc
     9181 aagctgcaca ccgatgaaat ccgggttgct gtaaacgcgg aaacttttgc cacagtgcag
     9241 caattgctgg aggtcatcgg caaaggtctg gtccgtcgag gccagcgaaa tggccgtcgg
     9301 taaacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca gcggaatttg
     9361 atcgcctaag gcctcacgcg ccacgtcctg taacagacgt ccggtttctg cttccagccc
     9421 tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct ggcgcagcac
     9481 ttcaccaaag acatggctgg gtacgacgac aagaatatta cggctggctg ccagcgcagt
     9541 ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga gaaacgcggc
     9601 gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc agaggacaac
     9661 ctcgtggcca tttcttgcca gggtgatggc aagagcggtg ccgtacgagc cggcaccgat
     9721 cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc agtaccttcg
     9781 ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg cgcaaggttc
     9841 agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc atacgggaac
     9901 agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc gatacccgcg
     9961 atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc gcccagggag
    10021 gccgttacgg taacacgcag taccacttcg taaacgtcat ctgccagttg agtagatgcc
    10081 gtatccagat caagtttaac ttctggttgc caatctttct ggaaaacgtg cggcgcgttc
    10141 ggcgcttcga aagaaatatc cttggtataa atacgttgga tctggaaagt catttcggtg
    10201 ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata ctgcgcaatg
    10261 taatgccaac gttcacacgt tatttcagca ggggatccag tccaccacgt gcatccaatg
    10321 catacaagtc atcacagcca ccaatgtgct gtgcgtcaat aaaaatctgt ggaaccgtgg
    10381 tgcgaccact gcgtttgatc atctcttcac gcttagcggc attgccatcg atcggcaact
    10441 cctgaaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa tacgggcagg
    10501 tttctttggt atagatttca acattggcca taaagtagct cctgtttttt accctgaaat
    10561 ttcatgttgc agggcggcag caaaatggag tgtccccaga agcttatatc aataagtcgc
    10621 tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa attatttgcc
    10681 gcgcaccaaa ggcaagtttt cgccagccca gccagcgacg ccttctttca gtacgaatac
    10741 ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc
    10801 atctaccacg ataaccggtt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt
    10861 gatttcgctc ggcaacaggt tgatagaacc tgcgatatgg cctttacgga agtcatcacg
    10921 ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg
    10981 agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag
    11041 taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg
    11101 catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga gtatacctgc
    11161 gcgttgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg gcgctacgaa
    11221 aaaaaagcgt aaatctggca tctgcttgcc gcgagcggtc gtatattttg atcttcagag
    11281 gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt
    11341 tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg gctatggcta
    11401 tcgcgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa tggatgcact
    11461 gtgggccaat cgtccgcata ccctgatcga cgcttccggt ttggaagtcg gtctgcctga
    11521 ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta
    11581 tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg ctaatccggt
    11641 gttgactggt gcagtagata aagcgaaaaa cgcaggcaaa gcagtacaca ttatgggtct
    11701 gctctccgca ggtggtgtac acagccacga agatcacatc atggcgatgg tagaactggc
    11761 agctgaacgc ggcgcagaaa aaatctacct acacgcattc cttgacggtc gcgacactcc
    11821 gccgcgcagt gctgaatcct cgctgaaaaa attcgaagag aaatttaccg cgctgggtaa
    11881 aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata accgttggga
    11941 tcgcgttgaa aaagcttatg acctgctgac tctggcgcaa ggtgagttcc aggccgatac
    12001 cgccgttgcc ggtttgcagg ctgcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc
    12061 gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg atgcactgat
    12121 tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg taaatgccga
    12181 ttttgacggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata
    12241 cgctgccgac atcaaaaccg cagtggctta cccacccgct tccctggtta acaccttcgg
    12301 cgaatggatg gcgaaaaacg acaaaactca gctgcgtatt tccgaaaccg aaaaatatgc
    12361 ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat
    12421 tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga gctccgcaga
    12481 gctgaccgaa aaactggttg cggcaatcaa gagcggcaaa tacgacacca ttatttgtaa
    12541 ctatccgaac ggcgacatgg taggtcacac cggagtgatg gaagcggcgg ttaaagcggt
    12601 tgaagcgctg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca
    12661 actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg caacgggtca
    12721 ggcacacaca gcacacacca acctgccagt tccgctgatt tacgttggtg ataagaacgt
    12781 gaaagcggtt gaaggcggca aactttctga catcgcgccg accatgttgt cgctgatggg
    12841 tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat aatccctccc
    12901 catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca ggtttgcaat
    12961 caggcccatc atctacgcca gcgttctgag cgctggcgta ttgttgtgcg ccttttccgc
    13021 ccacgcggat gagcgtgacc aactcaaatc cattcaggcc gatatcgccg cgaaagagcg
    13081 cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga aaaagcagga
    13141 agaagcgatc tctgaagcca ctcgcaaact gcgcgaaacg caaaatacgc tcaatcaact
    13201 gaataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc
    13261 ccaggagcgc agcctcgccg cgcaactgga tgccgcgttt cgtcagggtg aacataccgg
    13321 tattcagctg attctcagcg gtgaagaaag ccagcgtggg cagcggttac aggcttattt
    13381 cggctatctc aaccaggcgc gacaagaaac cattgctcag ttgaaacaaa cgcgtgaaga
    13441 agtcgctatg cagcgtgccg aactggaaga gaaacaaagc gagcaacaaa cgcttttata
    13501 tgagcagcgc gcccaacagg cgaagctgac ccaggcgttg agcgagcgta aaaagacgct
    13561 ggcagggctg gagtcttcca tccagcaagg tcagcaacag ttgagcgagc tgcgcgccaa
    13621 cgaatcccgt ctgcgtaaca gcattgcccg tgcggaagcc gcggcgaaag cgcgtgctga
    13681 acgtgaagcg cgcgaagccc aggcggttcg cgaccgccag aaagaagcga cgcgcaaagg
    13741 caccacctac aagccgaccg aaagcgaaaa atcgctgatg tcccgtaccg gtggtctggg
    13801 cgcaccgcgc ggtcaggcat tctggccagt tcgcgggccg acgctgcatc gctatggcga
    13861 acagctacag ggtgaattac gctggaaagg gatggtgatt ggtgcctctg aaggtactga
    13921 agttaaagcg attgccgacg gtcgggtgat tctggctgac tggctgcaag gctacggtct
    13981 ggtggtggtg gttgagcatg gtaaaggcga catgagtctt tacggctata atcagagcgc
    14041 actggtgagc gttggttcgc aggttcgcgc gggccagcca attgcactgg tgggcagcag
    14101 tggcggtcag ggtcggcctt cactctattt cgaaattcgc cgccagggtc aggcggtcaa
    14161 tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg ttcttgcatt
    14221 tgccgctctg ttggcgctct cctcccccgt aattgctggc aaacttgcca tcgtcattga
    14281 tgattttggg tatcgcccgc acaacgaaaa ccaggtgctg gcgatgcctt ccgctatctc
    14341 cgttgcggta ttacccgatt caccgcacgc cagagagatg gcgaccaaag cacataacag
    14401 cggtcacgaa gtgttgattc atctaccgat ggcgccactg agtaaacagc cgctggagaa
    14461 aaatacgcta cgcccggaga tgagcagcga cgaaattgag cgcattattc gtagtgcggt
    14521 caataacgtg ccctatgccg tggggatcaa caaccacatg ggcagcaaga tgacctctaa
    14581 cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tacaatcttt acttcctcga
    14641 cagcgtaacc atcggtaata cccaggcgat gcgcgccgcg caaggcactg gcgtgaaggt
    14701 gatcaaacgg aaggtgttcc tcgacgattc gcagaacgaa gcggacatcc gtgtgcaatt
    14761 taatcgcgca attgatctgg cgcgtcgcaa cggttcaacc attgccattg ggcatcctca
    14821 cccttcaacg gtacgcgtgc tacaacagat ggtttataac ctgccgccag acattacgct
    14881 ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acgtctacac cgccggaaaa
    14941 cactgtgccc gacacaccgc gtaatccatt ccgtggcgtg aagctgtgca aaccgaagaa
    15001 gccgctggaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca
    15061 gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac agcccgaagc
    15121 ggcaaaactt aacgctagcg caaattaaag gcactacgcg taatgcgatg gtgcgaccgc
    15181 gttttgtcgc gccattgcca taagcggaaa gaccacagta acgcctggta acccaccttc
    15241 acgctgcgca cattagtaat caggcgctta tacataccgg aagtaaatat ctcagcaatc
    15301 atccgttggc gggtaataat atccggctct ttgcgcaccg catggcaaac acgcaatgct
    15361 tcgtaagtta tttgctgatg aaattccgga taaatcgtaa ttttgtcggc ataatttcga
    15421 tttaatttct ccagcaggcg ggtaatctta atatagtgac gttgataatt aaggttttta
    15481 ttcccttgtc tatgcaaccg actcactgac gtattatgca gataatattt atataatgat
    15541 tgctcggtat atcgcgcacg cagcgcgtta aacataaatt ctgttgtcca gacaatatcc
    15601 tgatgatgta atccggcaat aaatttaatg ttatttttaa caataacatc gcggcgataa
    15661 acccccatcc agaccacgtg tgtccaacgg cgggaagaaa gccccatccg cagccagtcc
    15721 ggaccagtta atacaccggt tgagcgaagg cgatcggtag ggatggattg ccaggtttca
    15781 cccgtttcac gaaaacacca atcagcgttg cactgcgcca cgtcgaggtc gtcctctaac
    15841 gccatggtca tcagcgtttc gtacatggtg ggatacacct catcgtcagc atcgacaaaa
    15901 gcgacatatt ttcccgttgc cacttcaatc ccacgattac gcgccaccga tgcgccagca
    15961 ttcgcctgat gcaacaaacg aacgtgcgga tagttttctg cgtaatgctt tgctatttca
    16021 acagaattat ccgttgaacc atcgttaata ataatgattt ccagagcagt ccatgtttgc
    16081 gtaattaaag attccataca agtgcggaaa tcatcgcccg cattatataa cggaataata
    16141 acactaagtt tattggtgct gttcatcata aattaccgac gacctctgaa gttatcctga
    16201 cgttttacag gctttgtatt aagaaactat taagcctgtg agatattatt tttaccggtg
    16261 agagagtcac ttcagggtag agagataatt agagcagcta tataagagat cagcgcaatt
    16321 acgatatctt gattacactg acaggatttt acatatttaa cggattatcc tcaggcaaaa
    16381 gggctggaat accagccctt gttcgtgtta atcccagctc agaataactt tcccggactg
    16441 gcccgaacgc atggcatcaa agcccttctg gaaatcatcg atagagaaac gatgggtaat
    16501 gatcggcgag agatcgaggc cagactgaat cagcgccgcc atcttgtacc aggtttcaaa
    16561 catctcacga ccgtaaatac ctttaatgaa caagccttta aagattactt tggtccagtc
    16621 gatagacata tcggacggtg gaatacccag catcgcaata cgaccgccgt ggttcatggt
    16681 gtcgagcatg gtacgaaacg ctggcggcgc accggacatt tccagaccga catcaaagcc
    16741 ttcggtcatg cccagttctg tcatcacatc attaaggttt tctttagcga cgttaaccgc
    16801 acgggtgata cccattttgc gtgccagctc aaggcggtat tcgttaacat cagtgatcac
    16861 cacattgcgt gcaccaacgt gtttcgccac cgccgcagcc ataataccta tcggacctgc
    16921 accagagacc agcacatcct cgccaaccag atcgaacgac agcgcggtat gcacagcgtt
    16981 accgaaggga tcaaaaatgg aagccaggtc gtcggagata ttgtccggga ttttgaaggc
    17041 gttgaacgcc ggaatcacca gatactcggc gaagcagccc ggacggttaa caccgacgcc
    17101 gatcgtgttg cggcacagat gggtacgacc gccacgacag ttgcgacagt gaccgcaggt
    17161 gatatggcct tcgccagaaa cgcgatcgcc aatcttgaag cctttcactt cctgaccaat
    17221 acctaccact tcaccgacat attcatggcc cacgaccatc ggcaccggga tggttttttg
    17281 cgaccactca tcccagttat agatgtgaac gtcagtcccg cagatggctg ttttacggat
    17341 tttaatcagc agatcgttat gcccgagttc cggtacagga acgtcggtca tccagatgcc
    17401 ctcttccgct ttcagtttgg ataacgcttt catctcacac cctcaggcga taacgcccag
    17461 ttgtttacca atacgcgtga atgcttctac tgcacgcgta atttgctcag gggtatgcgc
    17521 cgcagacatc tgggtacgaa tacgcgcctg acctttagga acgaccggat agaagaaacc
    17581 ggtgacgtaa atgccctctt tttgcagctc acgggcaaat ttctgcgcca ctaccgcatc
    17641 gccaagcatg acgggaataa tggcgtgatc ggctcccgcc agggtaaagc ccgccgccga
    17701 catttgctca cggaactgac gcgcgttcgc ccacagacgg tcacgcagct cgctacccgc
    17761 ttcgaccatc tccagcactt tgatggatgc ggcgacaatc gctggtgcca gcgagttgga
    17821 gaacaggtac ggacgagaac gctggcgcag ccactcaacc acttctttgc gcgccgcggt
    17881 ataaccacca gaagccccgc ccaacgcttt acccaacgta ccggtaatga tgtctacacg
    17941 tcccatcaca tcgcagtatt catgggaacc acgaccattt tcaccgacaa aaccgaccgc
    18001 gtgggagtcg tctaccatca ccagggcatc gtatttatct gccagatcgc aaacgccctt
    18061 caggttggca atcacaccgt ccatcgagaa cacaccatcg gtggcaatca gcacatgacg
    18121 cgcaccggct tcacgcgctt ctttcagacg tgcttccagc tcctgcatat cgttgttggc
    18181 ataacgatag cgtttggctt tacacagacg cacaccatca ataatagacg cgtggttcag
    18241 tgcgtcggag ataatggcgt cctccgcgct cagcagcgtt tcaaacaggc cgccgttagc
    18301 atcaaagcag gaagagtaga gaatcgcatc ttccatcccc agaaacgccg ccagtttttg
    18361 ttcaagctct ttatggctgt cctgagtgcc gcaaataaaa cgcaccgaag ccatgccgaa
    18421 accgtgagaa tccattcccg cctttgccgc cgcaatcaga tccggatgat tcgccagtcc
    18481 gagatagttg ttggcacaaa agttaatgac gtggcttcca tcagccacag tgatatctgc
    18541 ttgctgcgca gacgtaataa tgcgctcttc tttaaacaac ccttccgccc gtgcggtttc
    18601 cagatcgttg gttaactgct gataaaattc tccacgcatt gcgattctcc agacagggca
    18661 aattccagca catattaccc aaacttatag gtcgcgacga gatgacgcgt taacacttct
    18721 gcaaaattca ggataaataa cgtccagccc cctcactttt ggtgaatggc tgaagccaac
    18781 gtcatgtagt gatatgatag aagtattcgt gtctgagatt gtctctgact ccataattcg
    18841 aaggttacag ttatgatcat cgttaccggc ggcgcgggct ttatcggaag caacatcgtt
    18901 aaagccctga atgataaagg catcaccgat attctggtgg tggacaacct gaaagacggc
    18961 accaagtttg tgaacctggt ggatctggat atcgcggact atatggataa ggaagacttc
    19021 ctgatccaga ttatggctgg cgaagagttc ggcgatgtcg aagcgatttt ccacgaaggc
    19081 gcgtgctctt ccaccaccga gtgggacggc aagtatatga tggataacaa ctatcaatac
    19141 tccaaagagc tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta tgcctcttcc
    19201 gcagccactt acggtggacg cacctccgac tttattgaat cccgcgaata tgaaaaaccg
    19261 ttgaatgtct acggttactc aaaattcctg tttgatgaat atgttcgcca aatcctgcct
    19321 gaagcgaact cgcagattgt tggcttccgt tatttcaacg tttatggacc tcgtgaaggc
    19381 cataaaggca gtatggcgag cgtcgctttc catctcaaca cccagcttaa taacggtgaa
    19441 tcaccgaagc tgttcgaagg tagcgagaac tttaaacgtg acttcgtcta tgtgggcgac
    19501 gtggcagatg taaacctgtg gttcctggaa aatggcgttt ccggcatctt caacctcggc
    19561 accggtcgtg cggaatcctt ccaggcagta gcagatgcta cgcttgctta tcacaagaaa
    19621 ggccaaatag aatacattcc gttcccggat aaactgaaag gccgctacca ggcgttcact
    19681 caggcagatc tgacgaatct gcgcgcggcg ggttacgata aaccgttcaa aaccgttgcc
    19741 gaaggcgtaa cggaatacat ggcctggctg aatcgtgacg cataagagct ctgcatgaaa
    19801 atactggtga tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag tctctatcgc
    19861 acgctccagg cgcgctatcc ccaggcgata atcgacgtga tggcaccggc atggtgccgt
    19921 ccattattat cgcggatgcc ggaagttaac gaagcgatcc ctatgcctct cggtcacgga
    19981 gcgctggaaa tcggcgaacg ccgcaaactg ggtcatagtc tgcgtgaaaa gcgctacgac
    20041 cgcgcctacg tcttacctaa ctccttcaaa tccgcattag tgcctttctt cgcaggtatt
    20101 cctcatcgca ccggctggcg cggcgagatg cgctacggtt tactcaacga tgtacgcgtg
    20161 ctcgataaag aagcctggcc gctaatggtg gaacgctatg tcgcgctggc ctatgacaaa
    20221 ggtattatgc gtaccgcaca agatctgccg cagccattgt tatggccgca gttgcaggtg
    20281 agcgaaggtg aaaaatcata tacctgtaat caattttcgc tttcatcaga acgtccgatg
    20341 attggttttt gcccgggtgc ggagtttggt ccggcaaaac gctggccaca ctaccactat
    20401 gcggagctgg caaagcagct gattgatgaa ggttatcagg tggttctgtt tggctcggcg
    20461 aaagatcatg aggcgggcaa tgagattctt gccgctttga atacagagca gcaggcatgg
    20521 tgtcggaatc tggcgggaga aacacagctt gatcaagcgg ttatcctgat tgcagcctgt
    20581 aaagccattg ttactaacga ttctggtctg atgcacgttg cggcggcgct caatcgtccg
    20641 ctggttgccc tgtatggacc aagcagcccg gacttcacac caccgctatc ccataaagca
    20701 cgcgtgatcc gcctgattac cggctatcac aaagtgcgta aaggtgacgc tgcggagggt
    20761 tatcaccaga gcttgatcga cattactccc cagcgcgtac tggaagaact caacgcgcta
    20821 ttgttacaag aggaagcctg acggatgcgg gttctgatcg ttaaaacatc gtcgatgggc
    20881 gatgttctcc acacgctgcc cgcactcact gatgcccagc aggcaatccc agggattaag
    20941 tttgactggg tggtggaaga agggttcgca cagattcctt cctggcacgc tgccgttgag
    21001 cgagttattc ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc tcccataaaa
    21061 gctgaacgca aagcgtttcg tgaagcgcta caagcaaaga actatgacgc agttatcgac
    21121 gcccaggggc tggtaaaaag cgcggcgctg gtgacgcgtc tggcgcatgg cgtaaagcat
    21181 ggaatggact ggcaaaccgc tcgcgaaccg ttagccagcc tgttttacaa tcgtaaacat
    21241 catattgcaa aacagcagca cgccgtagaa cgcacccgcg aactgtttgc caaaagtctg
    21301 ggctatagca aaccacaaac ccagggcgat tatgctatcg cacagcattt cctgacgaac
    21361 ctgcctacag atgctggcga atatgccgta tttcttcatg caacaacccg agatgataaa
    21421 cactggccgg aagaacactg gcgagaattg attggtttac tggctgattc aggaatacgg
    21481 attaaacttc cgtggggcgc gccgcatgag gaagaacggg cgaaacgact ggcggaagga
    21541 tttgcttatg ttgaagtatt gccgaagatg agtctggaag gcgttgcccg cgtactggct
    21601 ggggctaaat ttgtagtgtc ggtggatacg gggttaagcc atttaacggc ggcactggat
    21661 agacccaata tcacggttta tggacctacc gatccgggat taattggtgg gtatgggaag
    21721 aatcagatgg tttgtagggc tccgctaaag agtttaaata atttagaagc agcggctgtt
    21781 tataaagaaa ttacattgat ttaataaata gtaagtgggc attataccca cttacttaat
    21841 caacattttt attattgtgg ttctttttta ctcaaaaacg atatagacag gaagaggcac
    21901 acaatccacg atgccggtat tggtttagca tgcgtaatga catcactaat gccaaataac
    21961 ataatagaag cagggaaaac caaaagtata tattttttct cccggataca gtagtagatc
    22021 attgcaacat agaacataat aagaacaaga agaccaattt tccctttcgt ggaaagagct
    22081 tcaacaattt cattatgcat atgtacatca aggaataata aagctccact ataaatatta
    22141 ttttctgcac ttaatgcttt tatcttttca gcacgctgct caagagattg ccaattaaaa
    22201 ccatcaggac ttcctttaat tcctgcatta accattgcaa aacgtgctcc gaccgaggat
    22261 tgactgttat tttttgtagc atatgaaata acatcagctt ttaatgaatt aattcgccta
    22321 tcaatttcct tattaaatat caaaccacaa gcaagtagca atataaaaac accactcaat
    22381 cctttaaata aaaacacttt ttgatcacga tgctgaagaa ataatatgac cataatcata
    22441 agtgggaatg tgaaaattgc cgcacgcgtt cctgtcatca ttactgctgc aaagcttaaa
    22501 gcaaagttag cataaaatag aaaccattta tatttcgatt gcattttgat gaatacaatt
    22561 gaagaatata aagtagtgaa tgtaatcaca taagccgcaa tagtcgctgc ccccatctga
    22621 ccaatattcc cattcatggc ggagagggga atgcggtctg catgcaaata tatcccttg
//
LOCUS       DAZKHK010000087        22282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKHK010000087 DAZKHK010000000
VERSION     DAZKHK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.087"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..952
                     /gene="tehA"
                     /locus_tag="ACHOS5_003351"
     CDS             <1..952
                     /gene="tehA"
                     /locus_tag="ACHOS5_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP9778119.1"
                     /translation="GIVLGTIGMGFAWRYASQVWQVSHWLGDGLVILAMIIWGLLTGA
                     FITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLAVCLFSFGVVVQ
                     LAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTDAGLVFLGAGVF
                     SWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGGEGDTLAKMLFG
                     YGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSDNGFFHTLAVPL
                     FIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            949..1542
                     /gene="tehB"
                     /locus_tag="ACHOS5_003352"
     CDS             949..1542
                     /gene="tehB"
                     /locus_tag="ACHOS5_003352"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP9778120.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            1845..2513
                     /gene="ydcL"
                     /locus_tag="ACHOS5_003353"
     CDS             1845..2513
                     /gene="ydcL"
                     /locus_tag="ACHOS5_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP9778121.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2548..3759)
                     /gene="ydcO"
                     /locus_tag="ACHOS5_003354"
     CDS             complement(2548..3759)
                     /gene="ydcO"
                     /locus_tag="ACHOS5_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP9778122.1"
                     /translation="MLRYLYVITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LHEAIGVFIVTNVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLAWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYIPPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAIAGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCY
                     VVLNLIANRNR"
     gene            3812..4348
                     /gene="sutR"
                     /locus_tag="ACHOS5_003355"
     CDS             3812..4348
                     /gene="sutR"
                     /locus_tag="ACHOS5_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP9778123.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSSTPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            4421..6382
                     /gene="rlhA"
                     /locus_tag="ACHOS5_003356"
     CDS             4421..6382
                     /gene="rlhA"
                     /locus_tag="ACHOS5_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP9778124.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSVTHTLDGQFDEANNA
                     EKALNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(6474..6704)
                     /gene="yncJ"
                     /locus_tag="ACHOS5_003357"
     CDS             complement(6474..6704)
                     /gene="yncJ"
                     /locus_tag="ACHOS5_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HFP9778125.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            6883..7119
                     /gene="hicB"
                     /locus_tag="ACHOS5_003358"
                     /pseudo
     CDS             6883..7119
                     /gene="hicB"
                     /locus_tag="ACHOS5_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            7198..8604
                     /gene="ydcR"
                     /locus_tag="ACHOS5_003359"
     CDS             7198..8604
                     /gene="ydcR"
                     /locus_tag="ACHOS5_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9778126.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNVELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDHHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            8849..9994
                     /gene="ydcS"
                     /locus_tag="ACHOS5_003360"
     CDS             8849..9994
                     /gene="ydcS"
                     /locus_tag="ACHOS5_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP9778127.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHNLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10012..11025
                     /gene="ydcT"
                     /locus_tag="ACHOS5_003361"
     CDS             10012..11025
                     /gene="ydcT"
                     /locus_tag="ACHOS5_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9778128.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            11026..11967
                     /gene="ydcU"
                     /locus_tag="ACHOS5_003362"
     CDS             11026..11967
                     /gene="ydcU"
                     /locus_tag="ACHOS5_003362"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9778129.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            11957..12751
                     /gene="ydcV"
                     /locus_tag="ACHOS5_003363"
     CDS             11957..12751
                     /gene="ydcV"
                     /locus_tag="ACHOS5_003363"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9778130.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDSVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            12773..14197
                     /gene="patD"
                     /locus_tag="ACHOS5_003364"
     CDS             12773..14197
                     /gene="patD"
                     /locus_tag="ACHOS5_003364"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP9778131.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(14294..14389)
                     /gene="yncL"
                     /locus_tag="ACHOS5_003365"
     CDS             complement(14294..14389)
                     /gene="yncL"
                     /locus_tag="ACHOS5_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP9778132.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            14584..14757
                     /gene="ortT"
                     /locus_tag="ACHOS5_003366"
     CDS             14584..14757
                     /gene="ortT"
                     /locus_tag="ACHOS5_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP9778133.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            14843..15076
                     /gene="ydcY"
                     /locus_tag="ACHOS5_003367"
     CDS             14843..15076
                     /gene="ydcY"
                     /locus_tag="ACHOS5_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP9778134.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(15077..15526)
                     /gene="ydcZ"
                     /locus_tag="ACHOS5_003368"
     CDS             complement(15077..15526)
                     /gene="ydcZ"
                     /locus_tag="ACHOS5_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9778135.1"
                     /translation="MSQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(15523..16041)
                     /gene="mddA"
                     /locus_tag="ACHOS5_003369"
     CDS             complement(15523..16041)
                     /gene="mddA"
                     /locus_tag="ACHOS5_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP9778136.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            16222..16290
                     /locus_tag="ACHOS5_003370"
                     /pseudo
     CDS             16222..16290
                     /locus_tag="ACHOS5_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16290..16445
                     /locus_tag="ACHOS5_003371"
                     /pseudo
     CDS             16290..16445
                     /locus_tag="ACHOS5_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(16522..18624)
                     /gene="pqqU"
                     /locus_tag="ACHOS5_003372"
     CDS             complement(16522..18624)
                     /gene="pqqU"
                     /locus_tag="ACHOS5_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP9778137.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GINSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLTLFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMAFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            18866..19927
                     /gene="yncE"
                     /locus_tag="ACHOS5_003373"
     CDS             18866..19927
                     /gene="yncE"
                     /locus_tag="ACHOS5_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP9778138.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20040..21539)
                     /gene="ansP"
                     /locus_tag="ACHOS5_003374"
     CDS             complement(20040..21539)
                     /gene="ansP"
                     /locus_tag="ACHOS5_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP9778139.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVFAIVTFLVVGTVFLGSGQPLDGNATGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPVIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
BASE COUNT         5631 a         5485 c         5710 g         5456 t
ORIGIN      
        1 tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg
       61 gcaggtcagc cactggttgg gggatgggct ggtgattctg gcgatgatca tctggggatt
      121 attgactggc gcatttatta cccgactcat acgctttccg catagcgtgc tggcagaagt
      181 gcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc
      241 gattggtttt gttccgtggt ttcgcccact ggcggtgtgc ctgttcagtt ttggtgtcgt
      301 ggtgcaactg gcttatgccg cttggcaaac tgcgggatta tggcgcggat ctcaccctga
      361 agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat
      421 ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt
      481 ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg gagaattacc
      541 cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc
      601 ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt tcggttatgg
      661 actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa
      721 tgcatcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct
      781 ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt ttatctttac
      841 caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt
      901 gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt
      961 gatgaaaact attttactga taaatatgaa ttaacccgca cacactctga agtgctggaa
     1021 gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaat
     1081 agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt
     1141 atcgccaacg tcgagcgcat taagtccatt gaaaatctgg ataatttaca cacccgggtc
     1201 gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc gactgtggtg
     1261 ctgatgttcc ttgaggctaa aaccatccca gggctgattg ccaatatgca acgttgcact
     1321 aaacctggcg gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc
     1381 gtcggcttcc cgtttgcctt caaagagggg gaattacgtc gatattacga aggctgggag
     1441 atggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt
     1501 attaaactgc gtttcgccac gatgctggca cgtaaaaaat gatccggtaa gaacaaagcg
     1561 cgtgaaaatt ccccacgctg agatagttta ctgttcttct tttcggtaag catatttttt
     1621 atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagccgctt aacttactga
     1681 aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt cgtctgaaaa
     1741 ggggctaaaa aatttatttt caccaacact ttttttgcca caacacgaag cggcgctttt
     1801 tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt accacatcat
     1861 ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca
     1921 cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa gaaacaacct
     1981 cggcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa agcaaatatg
     2041 acagcatcgt ctggaaccca atcacttatt atccggtacc gaaaccttct acccaggtag
     2101 ggcagaaagt tctggataaa attttgaact ataccaacac cgaaatgaaa gaagcgatag
     2161 cgcagcgtaa accactggtt accaccgctg ggccgcgtag tctgattttc cgtggggcca
     2221 ttaccggtgt agataccagt aaagaagggc tgcaattcta tgaagtggtt cctgttgcat
     2281 tagtggttgc ggggacgcaa atggctacag gccaccgtac catggatact cgcttgtatt
     2341 ttgaaggtga gctgattgat gcagcgacta ataaaccggt tatcaaggtc gttcgtcagg
     2401 gcgaaggtaa agacctgaat aacgaaagta cgccaatggc tttcgaaaat attaaacaag
     2461 ttattgatga catggcgacc gatgccacca tgtttgacgt taacaaaaag tagttccaga
     2521 cgcgccatcg ttagatggcg ctttttatta tcggtttctg ttagcgatta aattcaacac
     2581 cacgtaacaa acgcctccgg caattaatcc ccaaaacgca gaaccaatcc cgaacagcgt
     2641 caatccactt gccgttacca gaaatgccac caccgccgcg tcgcgctcac gctcattatg
     2701 cagcgcctga tacaaactgc cgccaatggt actcaacagc gcaagacctg ccagcatctg
     2761 gatccaactt acgggcaggg cagccatcat cccggcaatg gcactaccaa acagacctgc
     2821 gatcaaataa aaaataccag caacggcagc ggccagccaa cgttgatctt tatccggatg
     2881 cgcttccggg ctttggcaaa tagccgcggt gattgccgca ataccgacgg aataaacgcc
     2941 gaaaggggaa aaaaacagtg ccagcaatcc agtaaacaca atcaacggcg aaacaggagc
     3001 cgaatacccg gctgctttca ttgctgcgat acccggtgcg ttttgcgatg ccatcgtcac
     3061 cagaaaaagg gggagtgcaa cgctcagact gtgagcaaac gaaaaatcag ggggaatata
     3121 agtggggaga acgggtttaa agacaacatc agttgtgaca acgtcacctt gcgcgataac
     3181 gatcacgatc ccaataatca tcgcggcaat taccgcatag cgcggcgcaa cggccctggt
     3241 tgccagccat gccagcaaca tactgccaca caatgtaaat tgaccatcca gactggcaaa
     3301 cgcctgtaaa ccaaaacgta ataaaatccc ggcaagcatt gccgccgcaa gcgagtgcgg
     3361 aataatgcgc atcagacgag caaagagtcc cgttatgccg cagaggacta ttaacacgtt
     3421 ggtgacaata aaaacgccga tggcttcgtg aagtgttagt ccctgtaatc cggtgaccaa
     3481 caaagccgca ccaggcgttg accatgcggt gagaacaggt acacgatacc ataatgtcag
     3541 agccagcgta ctgacgccca ttgccagccc cagcgccgtc atccagccag agatttgtgc
     3601 agtggtggct ccggcgacaa tcgctgcttg ccagattatt gccgctgaac tggcgtagcc
     3661 aattaatacc gccagaaacc ccgccagtag cgtgggtgga ggaatagaaa acagacgcat
     3721 agtcacctcg tgcgttataa cgtacaggta ccgtagcatt tgtccgttat aacgcacaag
     3781 tgataaactt ccgttttgcc ggaggagtcg catggaaaat ctcgctcgct ttttatccac
     3841 cacacttaaa caactacgtc agcagcgcgg ctggagtctt tcgcgactgg cagaagcgac
     3901 gggcgtttct aaagcgatgt tagggcagat tgagcgtaat gaatccagcc cgacggtcgc
     3961 gacattatgg aaaattgcca ccgggttgaa cgtgccattt tcaacattta tttctccgcc
     4021 gcagtcctct acgccttcag tttatgatcc acaacggcag gcgatggtga tcacttcgct
     4081 gtttccttat gatccacaac tctgctttga acacttctcg attcagatgg catccggtgc
     4141 aataagcgaa tcgacgcccc atgaaaaggg ggtaatcgaa catgtcgtcg tcattgatgg
     4201 acaacttgat ctgtgtgttg atggtgaatg gcagagcctt aattgtgggg aaggcgttcg
     4261 atttgctgca gacgtcacgc atgtctatcg taatggtggg gagcaaaccg tacattttca
     4321 ttccctcatc cattacccgc gcagttaagc gggaaaacta tttcgcaacg tcgcgcttgt
     4381 ggctaaaata gccgccattt ttcagctact ggataagaaa gtgaccgtat cttctcatcg
     4441 tcttgaactg ttaagcccgg cacgcgatgc cgccattgcc cgcgaagcta ttttgcacgg
     4501 tgccgatgct gtttatatcg gcggccctgg ttttggtgcc cgtcataatg ccagtaatag
     4561 cctgaaagat attgccgagt tggtgccgtt tgcccatcgt tatggtgcaa aaattttcgt
     4621 cacgcttaac accattttgc atgatgatga gctggaaccc gcgcaacggc tgattactga
     4681 cctctaccag accggtgtcg atgcgctgat tgttcaggat atggggattc tggaacttga
     4741 tattccgccg attgaactgc acgccagtac gcagtgcgat attcgtacag tagaaaaagc
     4801 gaagttcctc tctgatgttg gcttcacgca gattgtgctg gcgcgagagc tgaatcttga
     4861 gcagatccgc gcgattcacc aggccacgga cgcgaccatt gaattcttta ttcatggcgc
     4921 attgtgcgtg gcctattcgg gtcagtgcta catttctcat gcgcaaacag ggcgtagcgc
     4981 caaccgtggc gattgctcgc aggcgtgccg tttgccatac acattgaaag acgatcaggg
     5041 gcgggtggtt tcctatgaaa aacatctgct gtcgatgaaa gataacgatc agactgccaa
     5101 cctcggcgcg ctgattgatg ctggcgtacg ctccttcaag attgaagggc gttacaaaga
     5161 tatgagctac gtgaagaata tcaccgccca ttatcgtcag atgctggatg ccattattga
     5221 agaacgtggc gatctggcgc gcgcttcatc aggtcgtact gaacatttct ttgttccatc
     5281 gacggaaaag actttccacc gtggtagcac agattatttt gtgaatgccc gtaaaggcga
     5341 tattggcgcg ttcgattcgc cgaaatttat cggcctgccg gtgggcgaag tattgaaagt
     5401 agcgaaagat catattgatg ttgccgttac cgagccactg gcaaatggcg atggcctgaa
     5461 cgtgatgatt aaacgtgaag tcgtcggttt tcgtgccaat acggtagaga aaaccggaga
     5521 aaatcagtac cgcgtctggc caaatgaaat gccagcagat ttgcacaaaa ttcgcccgca
     5581 tcacccacta aaccgaaatc ttgatcataa ctggcagcag gcactgacaa aaacctccag
     5641 cgaacgccgg gtggcggtag acattgaact gggcggctgg caggaacaac tgattctgac
     5701 cctgaccagt gaagagggcg tcagcgtcac gcatacgctg gatggacaat tcgacgaagc
     5761 taataacgcc gaaaaagcgc ttaataacct gaaagatggt ctggctaagc tggggcaaac
     5821 catctattac gcccgcgatg tgcaaattaa cctgcctggt gcgctgtttg taccaaacag
     5881 tctgttaaac cagttccgcc gtgaagccgc cgatatgctt gatgctgcgc gtcttgccag
     5941 ttaccagcgc ggcagccgta aaccggttgc tgatcctgcg ccggtttatc cgcaaacgca
     6001 tctgagtttc ctcgcgaacg tatacaacca gaaagcgcgt gaattttatc atcgctatgg
     6061 tgtgcaactg attgacgcgg cgtatgaggc acatgaagag aagggcgaag tcccggtgat
     6121 gatcaccaag cattgtctgc gctttgcctt caatctgtgc ccgaaacagg cgaaaggcaa
     6181 tatcaaaagc tggaaggcga cgccaatgca actggttaac ggcgatgaag tattaacgct
     6241 aaagtttgat tgccgcccat gcgagatgca cgtcattggc aaaatcaaaa atcacatact
     6301 gaaaatgccg ttaccgggaa gcgtagtggc atccgtaagt ccggatgatc tgctgaaaac
     6361 attgccgaag cgaaaagggt aaaacgccag ttttcgggtt acttaccact catcaaatct
     6421 gcatgatatt gcctgccggg taaggcgtca tgccgcatcc ggcaacaaat gactcaacgc
     6481 tgtcccgctt ctggtttgcg cgatttttgc cagtaaaaat gctcgcgcaa accttccgcc
     6541 gactcttccg ccacagcacg caattcatcg ctgtccgctt catgacgcag ctgatgatcc
     6601 acattcttta cccacataaa ttcatgtcct ttgtgccctg ccatgagttg tcctgagaac
     6661 agtgcacacg ttaataagac aaccgataac gccttcgaaa acatcccgcc acctttttct
     6721 aaccttttgc cgctatgatg ccgatcgttt cttgaggtta ttattcagtt ttgcaaatta
     6781 gagcaaagaa attctggaat cttccttcct gattttgcat tgcattctgc cgttgcggcg
     6841 atttagtgct attttcgagc acattacaca cggaggtaaa cttcgccatt aaatagtcac
     6901 gatcacttta ttgaagtgcc tttaagtatc gcctctaaag tattgctgtt aaatgctttt
     6961 ttacagtcag aaatcactca gcaagagtta gccagacgaa ttggcaaacc taaacaggag
     7021 attactcgct tatttaactt gcaccatgcg acaaaaatcg acgccgtcca gcttgcggct
     7081 aaggcgcttg gcaaagagtt atcgctggtg atggtttgat aacagttaat gaaaagttgt
     7141 catttttaac aacggatata gactgccgaa tcatctgcac ataattacga ttcgataatg
     7201 aaaaaatacc agcagcttgc agaacaatta cgcgagcaga ttgcgtcggg tatctggcaa
     7261 cccggcgatc gtttgccttc gttgcgtgac caggtggcgc tttctggcat gagctttatg
     7321 accgtgagcc atgcctatca gttgctcgaa agtcagggat atattatcgc gcgaccgcaa
     7381 tcgggttatt acgttgcgcc acaggcaata aaaatgccga aagcgccagt cattccagtc
     7441 acacgagatg aagcagtcga tatcaacacc tatatttttg atatgttgca ggccagtcgt
     7501 gatccgtcgg tcgttccgtt tgcctcggcc tttcccgacc cgcgactttt ccccctccaa
     7561 caactcaacc gctcgctggc gcaggtaagc aaaaccgcca cggcgatgag cgtgattgaa
     7621 aacttaccgc caggaaacgt agaactgcgt caggctattg ctcgtcgcta tgccttacag
     7681 ggcatcacca tttctcctga tgaaattgtc attaccgccg gggcgttaga ggcattaaac
     7741 ctcagtttgc aagcggtaac tgaaccgggc gattgggtga tagtagagaa tccttgtttc
     7801 tacggcgcgt tgcaggcgct ggagcggtta cggctgaagg cgttatcggt ggcgacggac
     7861 gttaaagaag ggatcgatct tcaggcgctg gaactggcgt tgcaggagta tccggtgaaa
     7921 gcgtgctggc tgatgactaa tagccaaaat ccactcggat ttaccttaac gccgcagaaa
     7981 aaagcacaac tggtggcgtt gctcaatcag tacaacgtaa cgctgattga agatgacgtt
     8041 tacagcgaac tttattttgg acgggaaaaa ccgctgcctg cgaaagcgtg ggatcaccac
     8101 gatggtgttt tgcattgctc ttcgttttcg aaatgtctgg tgcctggttt tcgtattggt
     8161 tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct tgcagttgat gagtacgctt
     8221 tccaccagct caccgatgca gcttgcgctg gtggattacc tttctactcg ccgatacgat
     8281 gctcatcttc gtcgcctgcg tcgccagctt gcggaacgta aacaacgcgc ctggcaggct
     8341 ttgctgcgtt atctgcctgc ggaagtcaaa attcatcaca atgacagcgg ttattttctc
     8401 tggctggagc tccccgagcc gttagatgca ggagaattaa gcctggtggc actgacgcat
     8461 catatcagta ttgcgccggg taatatgttt tctaccggtg aaaactggtc acgttttttt
     8521 cgttttaata ccgcgtggca gtggggagaa cgtgaagaac aggcggtaaa acaattaggc
     8581 aaacttattc aagaacggct gtaatagcgt ttaatttaat tcctctcagg ttgggtaata
     8641 tgaatttcga atagcagtca tatatcctaa ctccttgtct atactccaga agataacctt
     8701 acagacggca taatgcgcgg tagctcacaa cctgattaaa ttttctcagg gccgaaggtg
     8761 tgcctgcaag ccgccgtcta tggttaaaca aggagatatt tttacggcac ggcggctgaa
     8821 caattaatta cgacaggagt aagaccttat gagcaagaca tttgcccgca gcagcctgtg
     8881 tgcgctcagc atgacaataa tgaccgctca cgccgccgaa ccgcctacca atttagagaa
     8941 tccggaaggg cgactggata ttatcgcctg gccgggatac atcgaacgcg gacaaactga
     9001 taaacagtac gactgggtaa cgcaattcga aaaagagaca ggctgcgcgg tgaatgttaa
     9061 aaccgccgcg acttccgatg aaatggtcag tctgatggcc aaagggggtt acgatctggt
     9121 tacggcatcc ggcgatgcct cgctgcgttt gattatgggc aaacgcgtgc agccaattaa
     9181 taccgccttg attcccaact ggaaagcgct cgatccgcgc gtggttaaag gcgactggtt
     9241 taacgttggt ggcaaagttt acggcacacc ttaccaatgg gggccgaacc tgctgatgta
     9301 caacactaaa accttcccga cgccgccgga tagctggcaa gtggtttttg ttgagcagaa
     9361 tctgccggac ggcaagagca ataaaggccg ggtacaggct tatgatggcc ctatctacat
     9421 tgcggatgcc gcgttgttcg tcaaagccac gcagccacag ttgggcatca gtgatccgta
     9481 tcaactcacc gaagaacagt atcaggcggt gctgaaagtg ctgcgcgatc aacataattt
     9541 gatccatcgc tactggcatg acactaccgt gcaaatgagc gatttcaaaa acgagggtgt
     9601 ggttgcttcc agtgcatggc cctatcaggc caacgccctg aaagccgaag gccagcctgt
     9661 cgctaccgtt ttcccgaagg agggcgttac cggttgggct gacaccacca tgctacatag
     9721 cgaagcgaaa catccggttt gcgcctacaa atggatgaac tggtcattaa ccccaaaagt
     9781 gcagggcgat gtggcggcct ggtttggctc gttaccagta gtgccgcaag ggtgtaaagc
     9841 cagtccgtta ttaggcgaga aaggttgtga aacaaacggt tttaactatt tcgataaaat
     9901 cgccttctgg aaaacgccta tagcagaagg gggcaagttt gttccctaca gtcgctggac
     9961 gcaggattac attgccatta tgggcggtcg ctaacttcgc tggggtgttt tatgacgtac
    10021 gcagtggagt ttgacaacgt ctcacggttg tacggtgacg tgcgcgcagt agatggcgtc
    10081 agtattgcga taaaagatgg tgagttcttc tctatgctgg ggccgtccgg ctccggcaaa
    10141 accacttgcc tgcgcctgat tgctggattc gaacagcttt ccggcggggc tatctctatt
    10201 tttggtacgc ccgccagcaa tctgccaccg tgggagcggg acgtgaatac tgtcttccag
    10261 gactacgcgc tatttccgca tatgtcgatt cttgacaacg tagcctacgg actgatggtc
    10321 aaaggcgtga ataaaaagca gcggcatgca atggcgcaag aggcgcttga gaaagtggcg
    10381 ttggggtttg tacagcaacg taaaccgtca caactttctg gtggtcagcg ccagcgagtc
    10441 gccatcgcca gagcactggt gaacgaaccg cgtgtgttgt tgctggatga accgctcggc
    10501 gcgctggatc tcaaattgcg agagcaaatg cagcttgagc tgaaaaagct gcaacagtct
    10561 ctcggtatca cttttatctt cgttacccac gatcagggcg aagcgttatc gatgtccgat
    10621 cgggtggcgg ttttcaataa tggacgcatt gaacaggtcg attctccgcg cgatctctat
    10681 atgcgcccgc gcacgccgtt tgttgcggga ttcgttggga catcgaatgt ttttgatgga
    10741 ctgatggcag agaaactttg tggcatgacg ggaagcttcg cactacgacc ggaacatatc
    10801 cgcctcaaca cccctggcga aatgcaggct aatggcacga tccaggcggt gcaatatcag
    10861 ggcgcagcga cccgttttga actgacactg agcggcggtg aaaaactgct ggtgagtcag
    10921 gccaatatga caggcgaaga actgcctgcc acgctcacgc ccggacaaca ggtgatggtt
    10981 tcctggtcgc gtgatgtgat ggtggcgctg gttgaggaga ggtgaatggc gatgaacgta
    11041 ttgcaatcac cttcacgtcc aggtctgggt aaggtgtccg gtttcttctg gcgtaatccg
    11101 gggctggggc tgtttttgct gctgcttggc ccgctaatgt ggtttggcat tgtctatttc
    11161 ggctcgctgc tgacgctgtt atggcagggc ttttatacct ttgacgattt caccatgtcg
    11221 gtaacgccgg aactgacgct ggcgaatatc cgtgcgctgt ttaatccggc gaattacgac
    11281 atcattgtcc gcacgctgac catggctgtg gcggtgacta tcgccagcgc cattctggct
    11341 tttccaatgg cgtggtatat ggcgcgctat accagcggaa aaatgaaagc gtttttttat
    11401 attgcggtaa tgttgccgat gtgggcgagc tacattgtta aagcctatgc ctggacgtta
    11461 ttgctggcaa aagatggcgt ggcgcagtgg tttttacaac atctggggct ggaaccactg
    11521 ctgactgcgt tcctcacgct accggcggtg ggcggcaata cgctgtcaac ttccgggctg
    11581 gggcgctttc tggtgtttct ctatatctgg ttgccgttca tgatcctccc ggtgcaggcg
    11641 gcgcttgagc gtttgccgcc gtcattgttg caggcgtcgg ctgatcttgg cgcacgtcca
    11701 cgacaaacct ttcgctatgt ggtgctgccg ctggcaatcc cgggtattgc cgctggctct
    11761 atctttacct tctcactcac gctgggcgat tttattgtcc cgcagctggt tggccctcca
    11821 ggatatttta tcggcaatat ggtttattcc cagcaggggg cgattggcaa tatgccgatg
    11881 gcggcggcat tcaccctggt gccgattgtt ctcatcgcac tctatctggc gtttgtgaaa
    11941 cgtctgggag cgttcgatgc actctgaacg cgcaccgttt ttcctcaaac tggcggcctg
    12001 gggcggcgtg gttttcctac attttcccat cctgattatc gctgcctatg cgtttaacac
    12061 tgaagatgcg gcgtttagtt ttccaccgca aggcctgacg ctgcgctggt ttagcgtggc
    12121 agcacagcgt agtgatatcc ttgattccgt gacactgtca cttaaagtgg cggcgctggc
    12181 gacattaatc gcgctggtgt tagggacgct ggcagctgcc gcgctgtggc gacgagactt
    12241 tttcggcaaa aacgccattt cgctgttact gttgctgccc attgcgctgc cgggcattgt
    12301 cactggtctg gcgctattaa ccgcctttaa aaccatcaat ctggagccag gatttttcac
    12361 catcgtggtc ggtcatgcga ctttttgcgt agtggtggtg tttaacaatg tcatcgcccg
    12421 ttttcgccgc acctcgtgga gtctggttga ggcgtcaatg gatcttgggg ccaatggctg
    12481 gcaaaccttc cgctacgtag tgttgccgaa tctcagttcg gcgttactgg ctggaggaat
    12541 gctggcgttt gccttgtcgt tcgatgaaat catcgtgacg acttttacgg caggtcatga
    12601 acgaacgtta ccgttgtggt tgctcaatca gcttgggcga ccgcgtgatg taccggtaac
    12661 caacgtggtg gcactgctgg ttatgttggt aacaaccttg ccgatcctgg gggcctggtg
    12721 gctaacccgc gaaggcgaca atggtcaata accactgata caggaatatg ctatgcaaca
    12781 taagttactg attaacggag aactggttag cggcgaaggg gaaaaacagc ctgtctataa
    12841 tccggcaacg ggggacgttt tactggagat tgccgaggca tccgcagagc aggtcaatgc
    12901 tgctgttcgc gcggcagatg cagcatttgc cgaatggggg caaaccacgc caaaagcgcg
    12961 tgcggaatgc ctgctgaaac tggctgatgt tatcgaagaa aatggtcagg tttttgccga
    13021 actggagtcc cgtaattgtg gcaaaccgct gcatagtgcg ttcaatgatg aaattccggc
    13081 gattgtcgat gtctttcgct ttttcgccgg tgcggcgcgc tgcctgaatg gtctggcggc
    13141 aggtgaatat cttgaaggtc atacttcgat gatccgtcgc gatccgttgg gggtcgtggc
    13201 ttctatcgca ccgtggaatt atccgctgat gatggctgcg tggaaactgg ctccggcgct
    13261 ggcggcaggg aactgcgtag tgcttaaacc atcagaaatt accccgctga ccgcgttgaa
    13321 gttggcagag ctggcgaaag atatcttccc ggctggcgtg attaacgtgc tgtttggcag
    13381 aggcaaaacg gtgggagatc cgctgaccgg acatcccaaa gtgcggatgg tgtcgctgac
    13441 gggttctatc gccactgggg aacacatcat cagccatacc gcgccgtcca ttaagcgtac
    13501 tcatatggaa cttggtggca aagcgccagt gattgttttt gatgatgcgg atattgaagc
    13561 agtggtcgaa ggtgtacgta cttttggcta ttacaatgcc ggacaggatt gtactgcggc
    13621 ttgtcggatc tacgcgcaaa aaggcattta cgatacgctg gtggaaaaac tcggtgctgc
    13681 ggtggcaacg ttaaaatcgg gttcgccgga tgacgagtct acggagcttg gacctttaag
    13741 ctcgctggcg catctcgaac gcgtcagcaa ggcggtagaa gaggcgaaag cgacagggca
    13801 catcaaagtg atcactggcg gtgaaaagcg caagggtaat ggctattact atgcgccgac
    13861 gctgctggct ggcgcattac aggacgatgc catcgtgcaa aaagaggtat ttggtccggt
    13921 agtgagtgtt acgctcttcg acaacgaaga acaggtggtg aactgggcga atgacagtca
    13981 gtacggactt gcatcttcgg tatggacgaa ggatgtgggc agggcgcatc gcgtcagcgc
    14041 gcggctgcaa tatggttgta cctgggtcaa tacccatttc atgctggtaa gtgaaatgcc
    14101 gcacggtggg cagaaacttt ctggttacgg caaggatatg tcactttatg ggctggagga
    14161 ttacaccgtc gtccgccacg tcatggttaa acattaagga taatattgca gatcgtaaga
    14221 gtatgagatg atcttgcgta ctgtcagagc actgatttct ctgacagtac gtgctgcgtg
    14281 caaaatcaca acatcaaata aaccagccaa atctcataga gataagagta aacaaaccaa
    14341 cagcagcaaa gacatttatc agtactacag ttctactgga aacattcatt tttgccacct
    14401 tttatcattg cccctatgtg tatagtacgg gttaagaaaa tccgcaataa tactcattct
    14461 gaagatagat aagggcaagt tgctgtttga tgattttttc tctaactggt gaaaaaactt
    14521 gatgcacgtc aaaaaatgac gcatatttgc gcgcgtttta ttcatctggc tggacgcccg
    14581 tacatgtctc tctatcaaca tatgcttgtt ttttatgcgg ttatggcagc aatcgcattt
    14641 cttatcacct ggtttctttc tcacgataag aaacgcatcc gtttcttaag cgcctttctg
    14701 gtgggggcaa catggccgat gagtttcccg gtggcgctgt tgttttctct gttttaaatc
    14761 ttatgcactg gcttgacggc accacgcaga cttatatcat ttggatgaat cgataaattt
    14821 cacaagtggc taaggagaaa gtatgtcgca tctggatgaa gtcatcgcgc gcgtggatgc
    14881 cgcgattgaa gagagcgtca ttgcccatat gaacgaatta ttaatcgccc tgagcgatga
    14941 cgcggagtta agtcgggaag atcgctacac ccagcagcag cgtttgcgca ccgctattgc
    15001 tcatcacggt cgcaaacata aagaagatat ggaagcgcgc cacgaacagt taaccaaagg
    15061 cggtaccatc ctctgattaa aatgagcgtc tggccaccag ccaggcgcca accacaagta
    15121 aaattgcgcc acaaattggg ccgatcaacg cccgaagtgg tacgccatgg ctgcggaaga
    15181 tatccagcac cagaccgcca atcagctggc tggcgaccag tactgcaata gttgtcgccg
    15241 ctcccacatt ctgatatccg ctgatactgg cgaagacaaa aaacgatccc agcaagccag
    15301 gtataagtgt ccaccagcgc acgctggaca ccagttcgcc aaaccccgcc atgccttgtt
    15361 taaaccataa aatagaaaca aagagcacaa tccccaccag tgagttcagc aacatggcga
    15421 tgagaatggt agaggaggtc tgggtgatgc gcaccatcag cgtgttctgg actaccagac
    15481 caatcccggc tgcaatcaaa aaggcaaggg taagtgactg actcatccaa tcgcgtccgg
    15541 ttcagtgcgc tcgtcgagtt gcaactgcat aaatgtcaga tccagccagc gcccaaattt
    15601 agtgcctacc tgcggcattt gcgcggtgac gacaaatccc agcgactggt gcagatgcag
    15661 cgaggcctga ttttgcgatt cgatcccggc gaccatgaca tgcttcccgc aatcccgcgc
    15721 ttcatcaatc aatcggctta acaatttacg ccccagacct ttcccctgat gatcgggatg
    15781 gacataaacc gaatgttcca cggtatggcg aaaaccatca aaactacgcc agtcgccaaa
    15841 cgaggcatat cccgtcacta cgccatcttc ttcactcacc agcaccggat aacctgctat
    15901 ggtccgcgct tcaaaccagg caatgcggtt atccgcatcc accgtttggt cattccagat
    15961 agccgccgta tacaacacgg cgtggttata aatttccgca attgcagcac agtcggcttt
    16021 gcgggcaaaa cggatggaca tgtctggcct cgataaaata atgatgatga tgttatcaat
    16081 actatcgaaa acagcccgaa gcaacggatt cctctatgaa attattgcgt gttgcagagg
    16141 ggatgtgacg gctgcaaaca aaaccaatcc tgtgagcctg ctactattga tggattcatg
    16201 ccaaaagcac aggagagcat tatggggcaa caaacgcagc gtaaccgatg ttgggttctg
    16261 gcctcgcgtc cgcatggcgc gccggttcct tacgtctggt ttcggtaaat gcgctctttg
    16321 tcgcaccagc gcaagcattt acagttccgg aagtgggaaa acgtcaatta aatgaaatca
    16381 atcggatccg ctacgaactg gaattaatgg cagttgctct ggctgtcgaa aacctcacta
    16441 tgcaaaaaaa cgttgctatg ttgtgccagc aatacagccg ataaataaaa tacgcccggc
    16501 aaataccggg cttaaagata attactcaaa tcgccacgca atatttatgc cgacaccata
    16561 atttcgtccg ggcgcaggtt cgtaatatcg cccgtttgac tcattgacaa tgacagaacc
    16621 aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacgg ttaaattgtg
    16681 gtaattgtat ttatacccgg tgaataagcc gacgagtgta taagaaggcg ctttggccgt
    16741 attttcatca tctgccataa tatcgcccat ataacgagct tccgtgcctg cataccaacc
    16801 atcttccggt atataaccta tcgacgcaaa ggccatatta cgggcgatcc ctggcatccg
    16861 gttaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat caagccaggt
    16921 ccaggacgct tttacgcgaa aatctcccgc gaaacgttga tcccatgcca gttcagcgcc
    16981 ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac tgctatcgac
    17041 aacaatttca tcatcggtgt cggtctggaa caatgtgaga ctaagcagcc catcaccaat
    17101 acgcgtttta ctgccgatct caattgtatc gttggtggag ggttttaaac caaagttcat
    17161 accgctttgc ccatcagcgc gataagacag ttcattaatc gtcggcgttt caaaaccgcg
    17221 cccggctgcc agatagacat tccaggcatc ggtcattgca tattttaacg atccggctgg
    17281 tagccatttg tgataactgg catcaccgct gtcgtcgccg ttacccggag taacgtaatg
    17341 atcattggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg aaagtttgtc
    17401 ggtcagttgc cactgggttt gtaaataggg atcgacgttc cacatcaaat tgcgttcgtc
    17461 gcggcgcaac tcaccttttt gcccgtattc cggcacgccg cgattcaggc ggaagttatt
    17521 gtaacccttg cggttttcac tcatgttttc gtagttcagg ccggttgtga acgtgactgg
    17581 aacgcccaat tcaccacggt gtgtccagcg gctgtttatc ccctggtaat ggcgttgcag
    17641 ggtaatcacg ccgcccgcat gtgacgggtt aagttgcggg gccatgggga ttgactggta
    17701 ctgggtcgtt tctcgctctc cggcatacat cattacactc atatcatctt gcgcgctcag
    17761 gctacgttca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg tgtcgtactg
    17821 ttctgcacgg ggcgcttgtt gtggattcgc cttccattct gcttcggtta gcccacctgg
    17881 gtcatctgct ttgatatcca cactattgaa aatcaggctt aatttgctgg cgtcatcaat
    17941 gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccgctatggt cacgatagcc
    18001 gtgggtcgta aaacgtgtgg ttgagacggt gtaatcgaca tcgccgggtt gtgtgccgtc
    18061 gcccgttgcg cccgtagctt tcagcccata gcgccagctg ccaaaactgc cgtagtaact
    18121 actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca ttacaccgcc
    18181 agacgcgttg ccatacagag cagagaaggg gccacgcagc acttccacat tttgcacact
    18241 gcttaaatcg atgttagatg tttgcccttg tccgtcgggc atggtagcgg gaataccgtc
    18301 cacatacagg cgaataccgc gaattccgta agtggagcgg gagccaaatc cgcgaatcga
    18361 cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgtaaaccag gcactccggt
    18421 cagtgattca gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc catccaccac
    18481 gctcacggct gctggggtat ccagttctga aaccacctgc ggtgcggcac tgacaatcat
    18541 ggtctgttca tcagcggcaa aaacaacggg ggaaaggaca agcagtgcgg gcaaaacggt
    18601 ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa aacaccgtca
    18661 tggtaatgaa attgtaaatt tatggaaaac gaaacggcgc aatacgttaa gtaattgaga
    18721 aaattgtagt cgtaacggta agaaatgctc cacatttgag aaaataatga ttaccattcc
    18781 catttataac aagggcgtaa cgatgattac gcttagcgaa gcattgtgaa gcagcaaaaa
    18841 tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat cgcgcctgcg
    18901 tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc aggccgcaga
    18961 agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata gccagcaaga
    19021 aaacgcgctg tggcttgcca cttcgcaaag ccgcaaactg gataaaggcg gcgtggttta
    19081 tcgtcttgat ccggttactc tggaagtgac gcaggcgatc cataacgatc tcaagccgtt
    19141 tggtgccacc atcaataaca cgactcagac gttgtggttt ggtaacaccg taaatagtgc
    19201 ggtcacggcg atagatgcca aaacgggcga agtgaaaggt cgtctggtgc tggatgatcg
    19261 taagcgcacg gaagaggtac gcccgttgca accacgtgag ctggtagctg atgatgccac
    19321 gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg ttgatggcga
    19381 gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg gtctggcgct
    19441 ggatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga ttaccatcga
    19501 caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca aagagcactt
    19561 ctttatcaac atcagccttg ataccaccaa tcagcgtgca tttatcaccg attctaaagc
    19621 ggcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg ttgcggcacc
    19681 ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtga cgcatcgtca
    19741 ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtggtgaaaa cgttcgatac
    19801 gccgactcat ccgaacagcc tggcgctgtc tgccgatggc aaaacgctgt atgtcagtgt
    19861 gaaacaaaaa tccactaaac agcaggaagc tacccagccg gacgatgtga ttcgtattgc
    19921 gctgtaatag atatggcgag ggtgaaaacg gagagacttg cttgactttt atcacgccgt
    19981 atccggcacc ttcgtcagta gtctgaattt gcccggcacg tagccgggca attttgcaat
    20041 tagctcgccg tttcaggctt aaacacaatt tcctgttttt cttcatcttc ctcgacgact
    20101 ggcgcagtgc tgtgaatttc agcaacgcgt ttgcgcacac caaaccaacc tataaccagc
    20161 agaataccga taaccggcag cgccgcgatg gtataagtcc cgttcgggta atcgaacgcc
    20221 atcagcacca ggacactcag taaaaacagt aatgtcagcc aggaagtgaa gggcgcgcca
    20281 ggcagtttaa aactgacatc cgctgctttg ccttctttaa tcgctttacg caggcgcatc
    20341 tggcaaacga tgataaacgc ccatgaagcg atgattccca gcgacgcgaa gttcaacaca
    20401 atctcaaata cgcgtgacgg caccagatag ttgaggaata cgccgacgac atacacaacc
    20461 agtgtcgcca gaatcccggc atacggcaca tgctgacgac tcattttcgc cataaaactc
    20521 ggtgcggaac cgcccatcgc catcgagcgc agaatacgcc cggtgcagta cagacctgaa
    20581 ttcaggctgg agagggcagc ggtcaacacc acaatgttca taatgctgcc gatgtacggc
    20641 acacccagtt tagagaaaaa cgtcacgaac ggactttgcc ccgcctgata cgcgctccac
    20701 ggcaataaca taaccagcaa caccacggag ccgacgtaaa acagaccaat acgccaaatc
    20761 acactgttaa tcgctttagg caccatggtc tgcggatctt tacattctcc ggccgctgta
    20821 cccaccattt caatggaggc aaaagcaaac actacgccct gaatcaacac cagcgcaggc
    20881 agcaaaccgt gggggaaaaa gccgccgtta tcggtaatta aatgaaaacc agtggcgttg
    20941 ccatccagcg gctgaccact accgaggaac actgtaccca cgaccaaaaa ggttacaatg
    21001 gcgaacactt taataagcgc aaaccagaac tccatctccg caaaccattt cacaccgatc
    21061 atattcatgg tgccaacgat ggtcagtgca gcgagcgcaa agacccactg cggcacgccg
    21121 ccaaacgcgc cccagtaatg catatacaga gcgacggcgg taatatcaac aatccccgtc
    21181 atcgcccagt tgatgaagta catccagcca gcaacataag cggccttctc acccaaaaac
    21241 tcacgcgcat aagagacaaa actgccactg gaagggcggt gtaacaccag ctcacccaat
    21301 gcacgcagaa taaaaaacga aaacaggccg cagatcaaat agaccagtgc cagtgcgggc
    21361 cccgccattt gcagtcgcgc ccccgcgcct aaaaacaaac ccgtaccaat cgcgccgcca
    21421 atggcgatca tctgtacctg gcgattgccc atcgctttgt gatacccctc ttcgtgggca
    21481 ttaagccagc gacgtttcgc ggcgtgttga tctgaagtgt cggtgttgtg tttactcatt
    21541 gctctccctg attgctttaa tgaaaaagtc atataagttg ccatgaacaa tgtttattca
    21601 tcctggcgat gacaagattc accattattc tttatgggta taactgtcgg cgcagaatcc
    21661 taccttttat catgtaacga tgcaaaataa tcccgctata aatatgtggg gataaaggcg
    21721 gctggcgaca aaatagcaac ctgatgaaat tatgacggaa acaaccacta ttggtttgat
    21781 gatacctgac tgcagtccta ttcttgcgcc actcgtgcgt cgctctgaac gccagtggtt
    21841 gcagtggaca ctggcgtggt tggctcaatg aatagtgagg ggctagcaca tgatgggttt
    21901 caggggtaat acgccaaata ccaatacaat tgctgattaa atgagccaat tgtcgacgtt
    21961 acagaaaagg ctaaaaaatg taagaggcat gtagggtgat aaacgcgcta ttgcccaaca
    22021 ggagtttctg gaattagtcc tgttccatgg gcattacaac tattaagaca ttacttcaat
    22081 ataattaatg tgtatatcat tctcgatatt aatttatgaa gtgaatttaa tatattccga
    22141 attcttaatt tacattacta atgtatgggg cataacgtta agatggctat gtgcagatta
    22201 agatatcagg gtggggcggt agcgtttaca gatactaatt aattgatggg aattatgtta
    22261 accatgcaat catttgcatc ga
//
LOCUS       DAZKHK010000088        22213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKHK010000088 DAZKHK010000000
VERSION     DAZKHK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.088"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(112..2541)
                     /gene="torZ"
                     /locus_tag="ACHOS5_003375"
     CDS             complement(112..2541)
                     /gene="torZ"
                     /locus_tag="ACHOS5_003375"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP9778140.1"
                     /translation="MTLTRREFIKHSGIAAGTLVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADISSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPTVQA"
     gene            complement(2566..3666)
                     /gene="torY"
                     /locus_tag="ACHOS5_003376"
     CDS             complement(2566..3666)
                     /gene="torY"
                     /locus_tag="ACHOS5_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP9778141.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVTPGTD
                     LTVVDASGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALIDPALADRKPLWQYAEKLDGTYCAGCHAPIAADHYTVNTWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(4054..4800)
                     /gene="cutC"
                     /locus_tag="ACHOS5_003377"
     CDS             complement(4054..4800)
                     /gene="cutC"
                     /locus_tag="ACHOS5_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9778142.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLKNLTELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(4814..5380)
                     /gene="yecM"
                     /locus_tag="ACHOS5_003378"
     CDS             complement(4814..5380)
                     /gene="yecM"
                     /locus_tag="ACHOS5_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9778143.1"
                     /translation="MANWQSIDELQDIATDLPRFIHALDELSHRLGLDISPLTADHIS
                     LRCHQNATAERWRRGFEQCSELLSENMINGRPICLFKLHEPLRVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            5596..7329
                     /gene="argS"
                     /locus_tag="ACHOS5_003379"
     CDS             5596..7329
                     /gene="argS"
                     /locus_tag="ACHOS5_003379"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9778144.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(7382..7774)
                     /gene="flhe"
                     /locus_tag="ACHOS5_003380"
     CDS             complement(7382..7774)
                     /gene="flhe"
                     /locus_tag="ACHOS5_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP9778145.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWHYQLIGPTPAGLRVRLCSQSRCVELEEQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(7774..9852)
                     /gene="flhA"
                     /locus_tag="ACHOS5_003381"
     CDS             complement(7774..9852)
                     /gene="flhA"
                     /locus_tag="ACHOS5_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9778146.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEAKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(9845..10993)
                     /gene="flhB"
                     /locus_tag="ACHOS5_003382"
     CDS             complement(9845..10993)
                     /gene="flhB"
                     /locus_tag="ACHOS5_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP9778147.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPLTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(11202..11846)
                     /gene="cheZ"
                     /locus_tag="ACHOS5_003383"
     CDS             complement(11202..11846)
                     /gene="cheZ"
                     /locus_tag="ACHOS5_003383"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP9778148.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(11857..12246)
                     /gene="cheY"
                     /locus_tag="ACHOS5_003384"
     CDS             complement(11857..12246)
                     /gene="cheY"
                     /locus_tag="ACHOS5_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP9778149.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGLDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(12261..13310)
                     /gene="cheB"
                     /locus_tag="ACHOS5_003385"
     CDS             complement(12261..13310)
                     /gene="cheB"
                     /locus_tag="ACHOS5_003385"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP9778150.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(13313..14173)
                     /gene="cheR"
                     /locus_tag="ACHOS5_003386"
     CDS             complement(13313..14173)
                     /gene="cheR"
                     /locus_tag="ACHOS5_003386"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP9778151.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLAETLSTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQKILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(14288..14418)
                     /locus_tag="ACHOS5_003387"
                     /pseudo
     CDS             complement(14288..14418)
                     /locus_tag="ACHOS5_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(14464..16125)
                     /gene="tar"
                     /locus_tag="ACHOS5_003388"
     CDS             complement(14464..16125)
                     /gene="tar"
                     /locus_tag="ACHOS5_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP9778152.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIITDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(16270..16773)
                     /gene="cheW"
                     /locus_tag="ACHOS5_003389"
     CDS             complement(16270..16773)
                     /gene="cheW"
                     /locus_tag="ACHOS5_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP9778153.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(16794..18758)
                     /gene="cheA"
                     /locus_tag="ACHOS5_003390"
     CDS             complement(16794..18758)
                     /gene="cheA"
                     /locus_tag="ACHOS5_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP9778154.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPDDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPMLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(18763..19689)
                     /gene="motB"
                     /locus_tag="ACHOS5_003391"
     CDS             complement(18763..19689)
                     /gene="motB"
                     /locus_tag="ACHOS5_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP9778155.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEEPEVAPQVSVPTMPSAEPR"
     gene            complement(19686..20573)
                     /gene="motA"
                     /locus_tag="ACHOS5_003392"
     CDS             complement(19686..20573)
                     /gene="motA"
                     /locus_tag="ACHOS5_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9778156.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(20700..21278)
                     /gene="flhC"
                     /locus_tag="ACHOS5_003393"
     CDS             complement(20700..21278)
                     /gene="flhC"
                     /locus_tag="ACHOS5_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP9778157.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSLNPADIIPQLLDEQRVQAV"
     gene            complement(21281..21631)
                     /gene="flhD"
                     /locus_tag="ACHOS5_003394"
     CDS             complement(21281..21631)
                     /gene="flhD"
                     /locus_tag="ACHOS5_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP9778158.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         5309 a         6102 c         5365 g         5437 t
ORIGIN      
        1 tcttgctaat tcaatatatt gcaggggcta tgtaggcctg ataagcatag cgcatcaggc
       61 agttttgcgt ttgcataacc tcagcgcccg tttccgggcg cttttcacgt cttatgcctg
      121 aacagttggc tgatcaaacg ccgttaactt cagcgcattg cccgtatatt tttcgatata
      181 caccagcgca gagttaccgg cacaggcatt cgccagctgc gagctggaga tatccgccgt
      241 taacacgttc gcactaccgt ttttacacaa gccattttcc agatccggcc atgcaccttc
      301 atgcacgcat accacgcctt ttttgatccc gtcagtcacc acagcgcctg tcaggatctg
      361 accgcgcttg ttccagacac gcaccagatc gccattcgca ataccaaagc gagcagcatc
      421 ttcggtgtga atagtgattg gttcacgatc ggcaaccgca tattttttac gcagttccgc
      481 atagttaagc tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt
      541 ctcgtcggcg gtacccttcc actcatcagg cgcaagccag gttgggtgtg ccgggcaatc
      601 cttatagcca aatttttcca gcgttctgga gtaaatctca attttgccgc ttggtgtacc
      661 cagcgcattt ttcaccggat cggcgcggaa atcaccataa cgaatgtact gttcgttctt
      721 ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag aaggcattaa acatcggcat
      781 agtcacgcgt tgcgcacggg cacctttctg agcggcatca tagaaaaatt tcagccacgc
      841 catttcatct ttaccttcgg tatagatctc ttttccgcca ggtttgagta attcagcaag
      901 atcggcaaac acgtcaaaat cgttgcgcgc ttcaaattgc gcagcgacag cctgcttcat
      961 cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc aggtcattgc gctcaaacga
     1021 tgtggtgatc ggtaatacga tatctgcgtg tttggctgct gcggtccagt agcattcaga
     1081 aacgacgatc atctccggtt tctgccatgc tttaatcaga cgattagtat cctggtgatg
     1141 ggtaaagtta ccgccacccg cccaccagat cattttgata tttgggtacg tttgctcttt
     1201 accgttatgc tgatatttgc cgccaggatt ttccagcgca tcgacgatac gggcaacagg
     1261 aatcgccgtc attccgccat catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga
     1321 catttctggt agcacgccgc caacacgcgt cgggttgcca ccgttggagt aatgataaga
     1381 gaaaccaaac ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag
     1441 catccagtgt ttctgttcac catattgctg gcgctgaatt ccccagcctg ccatcagcat
     1501 tgtgcgattt gccgccatca gttcagcgag tttgactatc tgcgcttccg gtacgccagt
     1561 aatttccgcc gcccaggccg cgctcttcgg cgtattatcg ctcttacctg tcagatactc
     1621 ttcaaactgc ggatacccgg tagtgtattt ttcaaggaac actttatcgt gtttgccttg
     1681 tgtcatcagg gtatgcgcaa tccctaacat cagtgccaca tcggtgccca tattcggtgc
     1741 gatccaggtg gcattatcgc caaagaattc gatggtttcg gagcggatag gatcaatggc
     1801 aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt gctcatcggt
     1861 actgctccag gcaattttga gcgtgttcag cgggttcatc ccccacagca ccaccacctg
     1921 gctgttttcc agaataagcg gccaggaggt ctgctgttca tacacctcta cagaaccgac
     1981 cacatgcggc atgatcacct gtgccgctcc ggttgaataa tcgccgctat gcccggaata
     2041 accgcccgcc aggttcatat aacgttgcag taaggtttgc gctttatgca acacgccaga
     2101 agaacgccag ccataagatc cggcaaaaat ggccgatggt ccgttagctt tgcgaatgcg
     2161 atcgtgttgt tcatgaatca gttttaatgc ttgttcccag ctcacctgta cataggtatc
     2221 ttcgccacga cctttcgccg gttgcagtgg attatcgaga tagctttttc tcaccatcgg
     2281 atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta aggagttcgg
     2341 tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg ccgtccttca cttctacgtt
     2401 catcgctccc caacggcccg cggtgagaat tttgccgccc ttttcttctg cccatgcggg
     2461 tagcggtgca gccgatgtca ccaccagcgt ccctgccgca ataccgctat gtttaataaa
     2521 ttcacgtctt gttaatgtca taacttcctc cctgatcaac gaggatcact gtttctcggt
     2581 aatatctttg gcgttgtact ggaagtaccg cgtcaaaatg tccagttcgt tttcgctcat
     2641 gctggttcgc gcccccattc ctttggcaat ggacggccat gtattgacgg tgtaatggtc
     2701 ggcggcaata ggggcatggc agccagcgca ataggtaccg tcaagttttt cagcgtattg
     2761 ccatagcggt ttacggtccg ctaatgcggg atcgataagc gccccctgca aagacgcctg
     2821 acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtta gcgccttgat
     2881 accttcttca cttaatgtgg cgagcgccag ccgttggcct gccgccaggt agagcgtgtt
     2941 ttcactgccc tgcatttgat aaccctgcaa ccgaacaatt ggctgtttgc cactggcatc
     3001 aacgacggta agatcggtac caggagtcac ggtagccagt tcgcctatgc gagaagtttt
     3061 gaaaggataa atatgtgcgc cattagtaac tgaagtagcg gcctgacttt ccagctcatg
     3121 cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt tatggcaatc
     3181 gatacaggtt tcgccgtcct tttgcgcttt attatgcatt ttctgcgcag attcactttg
     3241 cgaggcaata tccatggcat caaaagaatg gcaactacgg caggttgcag agtcagttgc
     3301 ttttaattct ttccatactg tttcggccat ttcctggcga tgagcttcga acttatcgtc
     3361 actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc
     3421 aaataaataa tccatccctg actttggaat atggcaatcg gcacattccg cacgtatccc
     3481 tttctggttt gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg
     3541 gcaggaaagg caaaatgcgg tatccgacgt tttatgtaag gctttttgcg ccagcaataa
     3601 cccgccgcca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc
     3661 tcgcatagat tcctctaatt aaattccagg acaccgatac cattgtataa ataaatacac
     3721 aacgaattga ctacttgctt aattcggcac aacactgcct gaaataatcg ataaagaata
     3781 tgatttatta caatgtaatc attaattgct aaggaataac ccagttgtta tttagaatat
     3841 atatttgtca gttttggcaa tttaaatcac ataatgaatg tgaatatata aaatattttt
     3901 agcacaacgc tatgcgcact gtcacacgct caacagatca cggcaatcgt tcgtttttta
     3961 tactgctcag ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc
     4021 atcatattgg gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat
     4081 tcctttcatt tcagcaaccg ccgccccgtc tacgacataa cgcgaatact cgtccgcgtg
     4141 tgcatctgat gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc
     4201 tcccgcggag ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttccgc
     4261 acggactcct gctccggcca taatgattgg agcatcacga tgggcaataa gttccataat
     4321 ttttgataaa ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc
     4381 caattccgta agattcttga gtgtatttaa agggttagcg cacatatcga aggcgcgatg
     4441 aaaagtcact gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac
     4501 attcccgtca acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggactgt
     4561 gcgcacatct tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg
     4621 tgggcggata atcggatgca ctgggatcgt cacccgctgg cgcacggatt tcagtacccc
     4681 caacgacggc gttaagcccc cctcttttgg agctgcgcat aattcaactc tgtcagcgcc
     4741 gttttgctgc gccgttagcg cgcattccat gctgtaacag caaatttcca gtaatgccat
     4801 ttttgctcct tagttacgcc gactgctcgc tggcaacgat ctcttcaatg gaccacggat
     4861 gaaacttaat ggttgtttta ccatcggtga ccgccagcgt cggattcggt aatcgttcat
     4921 gctcaccttt tggcgaactg gttttaactg agatccccgg caggctgagt ccttcatcag
     4981 aaagcaatgc cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa
     5041 tatgttccca accttcgtgc gggtaacgtt tttccccggg ccacggtaac tcaacaatcg
     5101 aaaactgcca gtgagcaacc cgcagcggct catgcaattt aaacaggcaa atcggtctac
     5161 cattgatcat attttctgac aggagttcgc tgcactgttc gaatccgcga cgccagcgtt
     5221 cagcggtggc gttttgatgg caacgcaagg aaatgtgatc ggcagtcagc ggggagatat
     5281 ccagccccag acggtgggaa agttcgtcta atgcgtggat aaatcgcggt aaatccgttg
     5341 caatatcctg caactcgtcg atagattgcc agtttgccat aatcactctt cgtctttcag
     5401 taaaagcgtt aatttaccct gttgccctgc gccaaccaac cgctgatttc gcgccgctcc
     5461 tgatgcaata gtgaaaacgg caatacgccc cgcgcacgtt gctgacgaaa acagccattt
     5521 gcagtatact cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca
     5581 atgtaaggta ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg
     5641 attgcggcag gcgcgcctgc ggattgcgaa ccgcaggttc gtcagtcagc aaaagttcag
     5701 ttcggcgact atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg
     5761 cgacaattag cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt
     5821 gagatcgccg gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat
     5881 gttcagcagg cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acaaaccatt
     5941 gtggttgact actctgcgcc aaacgtggcg aaagagatgc atgtcggtca cctgcgctct
     6001 accattattg gtgacgcagc agttcgtact ctggagttcc tcggtcacaa agtgattcgc
     6061 gcaaaccacg tcggcgactg gggcactcag ttcggtatgc tgattgcgtg gctggaaaag
     6121 cagcagcagg aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat
     6181 gcgaaaaagc attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta
     6241 aaactgcaaa gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc
     6301 atgacgcaga accagatcac ctacgatcgt ctgaacgtga cgctgacccg tgatgacgtg
     6361 atgggtgaaa gcctctacaa cccaatgctg ccgggtatcg tggcggacct gaaagccaaa
     6421 ggtctggcag tagaaagtga aggcgcgacc gttgtattcc ttgatgagtt caaaaacaag
     6481 gaaggcgaac cgatgggcgt gatcattcag aagaaagatg gtggctatct ctacaccacc
     6541 actgatatcg cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat
     6601 tacatcgatt cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagcg
     6661 ggctatgtac cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggcaaa
     6721 gatggtaaac cgttcaaaac ccgcgctggc ggtacggtga aactggccga tctgctggat
     6781 gaagccctgg agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagccgac
     6841 gagctggaaa aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc
     6901 aaaaaccgca ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat
     6961 accgcgccat acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa
     7021 attaacgaag agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa
     7081 ctggcagctc gcctgctgca gtttgaagaa accctcactg tggttgcccg tgaaggcacg
     7141 ccgcatgtga tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag
     7201 cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca
     7261 caactgacgg cgaagacgct gaagctgggt ctggatacgc tgggtattga gacagtagag
     7321 cgtatgtaat cgcgttatac ggcaacagcc gatgtcattg cagacatcgg cttttttatc
     7381 attaacgata atttacaatc acttcattcc gttgaatctt tagcggcgga attaatcgcc
     7441 caccgccagg cacttcccag ataaagcgca acggttctgc cgccggtata ccggaaaagg
     7501 ccacggtggt tccactctgc tcctctaatt cgacacaacg agattgtgaa cacaagcgaa
     7561 cccgcagtcc tgcaggtgtc ggaccgataa gctgataatg ccacgccacc agcgtcatca
     7621 atcctgaagc aggttgtcgc gtagaaagag gcgctgacga catcgattca ccgcgatgat
     7681 ttagcgtaac gcccacgcta ctcgcctgcc acatcccctc cccggcggct tgcaccagca
     7741 gcggaaacaa taatattgct aataaggctc tcattatttg ccgccaatcg tcgccgtcat
     7801 acggatatgt cggttatctg atagttccag attcgacagc accactaact gcggcaagct
     7861 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca gtaccggcgg
     7921 cgcacccaac atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
     7981 cagccctggc tccagtcccc ccccgccctg caatgcctgt agcaacaaac gttccagcgg
     8041 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
     8101 ccgtcccaac gctacgcgca cgacggcggt taattcatgc ggatcgcttt ggatgggcgc
     8161 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggcactt tttcatcgag
     8221 cagattttgc agcactttat gcagtgtggt aagcgtgacg acgccaggaa cgagatcttc
     8281 cgtcagcttt ggcatctcct gggcgacgcg atccagcagc tgttgcgcct cctgacgacc
     8341 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcca ccaccgtgct
     8401 ggcctcaacc acggtgtacc cctgaatctg cgcctgctct ttaagcgcac tttcaatcca
     8461 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     8521 ggttccaggg ttaatcgcca accaacgccc cggataagca tcaccactgc ctatctccac
     8581 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgtac
     8641 cactggcggc agaaacccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     8701 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     8761 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     8821 tgccattttt accggttttg cttcggcagg cgctttttgt tcacgtccgc ggatccacca
     8881 cgccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     8941 caggccgagt aaaccgagca cggcggcgct taataacata acgctcggat tactgaaaag
     9001 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgtgtaa cgatgacgcc
     9061 cgcggcggta gaaatcacca gtgccgggat ttgtgccacc agaccgtcgc caatggtcaa
     9121 tagcgtataa ctttccgccg cgtgccccat gctcatgcca tgttgcagca caccgaccag
     9181 caatccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat cgccgcgaac
     9241 aaacttactt gccccgtcca tcgagccgta aaaatcggct tcctgagtca cttcggagcg
     9301 gcgttttttc gcctcatctt caccaatcaa tccggcatta aggtcggcgt caatcgccat
     9361 ctgcttaccc ggcataccat cgagaacaaa gcgcgcgccc acttccgcaa tacgcccggc
     9421 acctttggtg ataaccataa agttgatgat cacgagaatg acgaacacca cgataccgat
     9481 agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accacctttc cagccgccgc
     9541 cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa gtgccagacg
     9601 caacagcgtg gtaaacaaca ggatggtcgg aaacgcagca aactcgagcg tgcgctgggt
     9661 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acagcaggtc
     9721 gagtatgaat gcgggcagtg gcagtaccat catcgacaag atcaacagga tcaaaatcgg
     9781 tccggcaagg atctgccatt gtgtcgattt caggtttgca ggcaggcgca gcatcgcggc
     9841 cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc accggaagat
     9901 gagtaggttg tacagggcgt tgtccaccag ccagacgcca gcgtttcagt tgccagaccc
     9961 aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga ccgatctccg
    10021 catgtcgata cagcgcacgc gccagcggcg gcgcttcaag cgtcgggacg ttattttcag
    10081 cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc actttcggtg
    10141 cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg ttattgacaa
    10201 tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcggccgct cgctgcatct
    10261 gacggatccg ccctttaaca tgggggtcgc cttcgctttg tttgaactca tcacgaatat
    10321 cctgccgtga cattcgcagc tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc
    10381 ccaccattgg aatgacacca agcaccacca gcagcgcgca tagccctacc agatccatcg
    10441 cattacccat ggcggtaagc ggagactcgg ccattaagcg catcatctgc ggccagtgat
    10501 gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt gccttaagca
    10561 actccgcgcc agtctgtgcc gagaacatcc gtttgatgcc cggtagcggg ttgagtttgg
    10621 aaaacttcgg ctgcaaggat tttccgctaa ataccagccc ccccagcata accggagaaa
    10681 taatcgccac cagcaccacg ccgctaatca gcggtagcag cgccagcatg gcttctctga
    10741 tcagcagaat aatctgcccg aggatcagat tcgggtcatt gataatactg tgatcaaaat
    10801 gcagccctgc ggagagcatg cccgacaatc gacgggccag cgacacaccg ccaaaccaga
    10861 taacactaac gcccaccagc aaaatcagca gtgaagtcag ttcacgggaa cgcgggattt
    10921 gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc
    10981 tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtaaatgat gccagagcgc
    11041 aaagtgttca atggtttgag taaggggcaa aacaggcggg atttagggct tttgccacag
    11101 cacatcaggc atggtgacat gtttgtcggg tacggcgtaa acgccttatc caaccttgaa
    11161 gatctgatgt gacgcggcca cgtcacatca ggcaatacaa atcaaaatcc aagactatcc
    11221 aacaaatcgt ccacctgatc ctgactggct accacaccgg ctttgctggt atcgacctga
    11281 ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt
    11341 tccaacagca ccatcagcaa ctgacgttcg atctcctgaa tgacatccat catccgctta
    11401 atgacctgtc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg
    11461 ttagtaaagc tggtatgtgc ggggacatct gccagaaatt gccgcgtgtc tgttaccagt
    11521 tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac
    11581 gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc
    11641 gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg
    11701 gggatagctt ccgctgcttc ggcaatggcc tgatccagcc ccagttcccg caaactgtcg
    11761 cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca
    11821 gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat
    11881 tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggt ttcaccacat agccactggc
    11941 ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca ccattaacac
    12001 tggcaatgcc gacatcgcgc catccgcacg aatcgttttc agcaactcca ggccatccat
    12061 attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag
    12121 agcgtcgagg ccatcttccg cttcctcaac attattgaat cccagctctt tcagcaggtt
    12181 acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc
    12241 cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat ttttgccagc
    12301 atttgctgac ttacctggct aagatcgacc acttcgcaga caccacccat attgatggcc
    12361 tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt ccatgccccc
    12421 gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccatgcccgt caggatcacc
    12481 ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgatggc
    12541 cgatggcgat taaccgccgg gccatcgtga attttgattt gataattcgc gccgctacgc
    12601 gccagctcca tatgccgatc gcccggcgcg atataggcat gtcccggcaa gacgcgctct
    12661 ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag
    12721 cgggtgaaac cgggcggcat atgctgggta attaacagtg ccggactgga aagcggcaac
    12781 ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt
    12841 ttttcagaac tcaacaacgg ccccgccttc agcgttgtcg gtgccgacaa tggcttatgt
    12901 gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc
    12961 agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctatcgc ccccaactcc
    13021 agcgcgcgca gcgtgacttc tgaccctttg ccggtcaggg aagaaaccat cacaacgggc
    13081 attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca
    13141 acatccagcg tcaatacatc gggattgaat ttcttaatca aatcacgcgc aaccagcgga
    13201 tcaggggcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc
    13261 tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct
    13321 tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt
    13381 tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca
    13441 aaatcttctg ctgggtagtt tgatcgaagt agatcatgac gttacgacag aagatcgcat
    13501 caaacggccc cggcacggtg tactgtttcg ccagtaaatt cagcggggca aaatcaacat
    13561 agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga
    13621 aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc
    13681 ttctggcttt ttccagcact tcggtgtcga tatcgctggc aaacactttc cagcgtccgg
    13741 gcacggtgct caatgtttca gccagcgtca tcgcaatgct gtacggctct tcgccggtcg
    13801 aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga tccgcgagca
    13861 gaggaaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa
    13921 acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcaggtaa tgaccgaaat
    13981 ccgccagtcc cagcgcacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat
    14041 ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg
    14101 cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca
    14161 gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact
    14221 gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc acacgcgacg
    14281 aaagatggtc ggcctgattc agtcgttgca aatcgtcgcg aaatgcccaa aagacatgcc
    14341 gttactgcca atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat
    14401 tcgaatacga ttaaacatca acgctcccca tcaggcggca atgaccgctt tagtaaatac
    14461 tcgtcaaaat gtttcccagt ttggatcttg ttcagcaatt cgcagtcgtg gctgtgccgg
    14521 tggttgctca ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc
    14581 cagacggaac gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc
    14641 ggcggcagct gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga
    14701 aaccgccaat gcgacttgat cgatgccacg gctctgttca tccgatgctg atgcaatctc
    14761 gcccataatg tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccagc
    14821 gctttcgacc agcaccgaac cggtatcaac gcgcgagacg gagtcttcaa tgagggcttt
    14881 gatctctttt gccgcctgtg cactgcgact ggcaagatta cgcacttcac ccgccaccac
    14941 ggcaaaacca cggccctgtt cacccgctcg cgcggcttca acagcggcat tcagcgcgag
    15001 gatattagtc tggaaggcaa taccatcgat aacgctgata atgtcggcaa ttttcttcga
    15061 actatcggcg atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg
    15121 ggcggtgtcg gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg
    15181 cttcactgtc gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg
    15241 ctgttcagta cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata
    15301 gatggcatct gaaccttcgc gcacatgagt gacggtgtca gtcaaagagc gttgcatatg
    15361 tgaaacactc tgcgccaggt cgcccatttc actgcgcccg tcaatggtca gggtattcgc
    15421 caggttacca ccggcaattt cgcgaatatg cgcaataatt tttgccagcg gtgtaagcaa
    15481 catacggcga atgccgtacc acgccaccag cagaatcaat accaccacca gcgcgataac
    15541 cgccagttgc cactgggcaa atcggtaatc atctgcgttg tcagtgatga tatcgcgata
    15601 cagtttttca ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc
    15661 ctgggttggc tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc
    15721 agttaacgct gtgtgatagt ttttatattt ttcatcaata ttacgactgg tagcgaccat
    15781 ttcaggtaac ggtgccatgc ttttgaattt tttataatga gtcgctgcct gggccaatgt
    15841 tttcctggcg ctatcgagca attcaacttt ggcattactt tgctgattgg atgaatccat
    15901 catcatccgc accgctgaac gactcaggtt aatgcgcgtt tgcagcatta aatcccatgt
    15961 tgacgtcagc tcgccctgct gttcccgtaa ttgattggaa accacaaagc tcttctggct
    16021 atggtgaagg gaagaaaaaa acagactgcc ggaaataagc tgtaacagtg cgaatacccc
    16081 cagcaccatt accaacagcg tgactacgcg gatacggtta atcataaggc accttcctga
    16141 aaacaagttg atctcgttat cggcaaggaa gggggaaact ttattgctga tgccacccgc
    16201 cgcgaaactg aaataaaaaa cccgatgcgc aggtcatcgg gttcattgca attgaggaaa
    16261 tcgggagaat tacgccactt ctgacgccgc gctatctaac agcgccatct cttcgctgtt
    16321 cagcagtttt tcgatgttca ccagaatcaa catgcggtcg ccgagtgcgc ccagtcctgt
    16381 gagatattcg gttgaaagcg tcacggcaaa ttccggtgcc ggacgaattt gctccgccgt
    16441 caatgaaagc acgtctgaga cgccgtcaac cacgatgccg accacccgct gtccgagatt
    16501 caggacgata actaccgtgt tgtcgttata gtccacatcc acctggctga acttaattcg
    16561 taagtcaaca atcggcacaa taacgccgcg cagattcgtg acgcctttga taaacgctgg
    16621 cgtgttcgca atccgtgtca cctgatcgta gccacggatc tcctgcactt tcagaatatc
    16681 aataccgtac tcttcatcac caagggtaaa taccagaaat tcctggcctg acggctcgct
    16741 ggccagcttt gttacattcg tcataccggt catattgtta cctttttact cattcaggcg
    16801 gcggtgttcg ccatacgttg ttcgcggttt atcgcctgca aggcggagac atcaacaatc
    16861 agtgccacgc tgccgtcgcc aagaatggtc gcagcagaaa tgccggggac tttgcgatag
    16921 ttactttcaa ggtttttaac tacaacctga tgttgaccaa ttaattgatc caccagcaag
    16981 gcgtagcggc gaccgccact ttgtaagatc accacaattc cctgggtagc ttcggttttc
    17041 gcgcccgcga cgttgaacac tttccacagt tcgacgatgg gcagatattc accccgcact
    17101 tccagcaccc gctcgccgcc ggccagtgga tggagatcgg cttcacgggg ttgcagtgat
    17161 tccataacag cattcagcgg cagaatgaaa acttcatccg caacgcgtac ggacatgccg
    17221 tcgaggatgg ccagcgtcag cggcagtaaa atgcggatcg tcgtgccagt accctgcttc
    17281 gactggattt cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg
    17341 acgccgcgcc cggagacgtc ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc
    17401 agcatcgcga cttcgtcgtc gctcatgttt tcgctgacgg tcagaccttg cgaggccgct
    17461 tttgccagaa ttcgttcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg
    17521 ttgccgccct gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcagcg
    17581 agccgttttt ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc
    17641 gggtcgataa tgcgttcaat caggctcttg tcgagttcag tagaactgcc caccagcgtc
    17701 agttctacct gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta
    17761 aagacatatt ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg
    17821 ttacgttgta actgccccat gctggtaatc aaatcaccat gattaaccgg gtccagttcg
    17881 ctggaacgct gggcaagcat ggactgggtg atgaccagct caccgacgag gttaattaat
    17941 tgatcaacct tttctaccgc tacacggatg ctggtggatt cactgctgcg cgtcgttttt
    18001 tcccgctcca cgcgaccggt tggcgcttgt tcggctgcca gtttaagcat tggtggggtg
    18061 gatatttttg gcgagacgtc tactgtttca aaggtaatct gatcggcttc aatcacaaaa
    18121 cagagtaccg cagtgatgtc atcttcggcg atgtcgtccg gtaatattgc cgagagagaa
    18181 tcagcccctt tcaccacgtc agttaacgtt gtcagatgcc ccaactcttc ttccagcagg
    18241 tcgacttcgc cggccttcag gcgcgaaagg ataattcgtc gcggtgactg actacgactc
    18301 tgctcatctt gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt
    18361 tcgcctttcg cttctaatgc cagttgacgc aaggcctggc agatataatc gaagctggca
    18421 gcatccggct cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt
    18481 tccaaaaaca gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg
    18541 agcaggtttt ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa ggttcctgcc
    18601 cctcctttga tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct
    18661 tccggctgca aaaccagcag atgctgctcc atatcagcca acagttcgtc cgcttcatca
    18721 aaaaatgtct gataaaaatc gcttatatcc atgctcacgc tgtcacctcg gttcggctga
    18781 tggcattgtg ggaacactga cctgtggtgc aacctcaggt tcttccaggg cgcttactgg
    18841 ctcattctgg ctttcggcgt tttcatgcaa aatggcctgt tcggcgtgtt tgttcagtac
    18901 cagcaggctg atgcgacggt tgacagcatc atcaggaccg cgatcgctta agcgcatcgt
    18961 tgccgccatg ccgacgacac gtaacacttt gccgccattc aacccgccaa ccatcagttc
    19021 gcggcgggat gcattggccc gatcggcaga aagctcccag ttgctatatc ctttctcacc
    19081 actggcgtaa gggaaatcat cggtatgacc cgaaaggcta atacggttgg gaataccatt
    19141 cagaacgggc gcgatggcgc gcagaatgtc gcgcatatag ggttcgacgt cggcactgcc
    19201 ggttctgaac atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac
    19261 cagatcgatt ttgagatgtg gacgtaacgc ccgcagtttc ggatcggact ctatcaattg
    19321 gtcgagatcg ccacgcaatt tccgcaatcg actttgctcc atgcgttttt tcagctcttc
    19381 gatgttcggc tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg
    19441 aattgggctt tcactattag aaatgcgatc gccgcccgta accgctgtcg ccagcggagt
    19501 ccggaagtac tccgcaatct gaatcagctc ttttggactg gagatagaga tcagccacat
    19561 caccagaaaa aaggccatca ttgcggtcat aaagtcggca taagcaatct tccacgatcc
    19621 atgtgctgcc ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg
    19681 attcttcatg cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc
    19741 tcttccagct caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca
    19801 acggcgatag gcggtgcgta accgttcaga tttgaaagca gagtgacttt gacgcactgc
    19861 atcattttgc tggtttcggc gcttttctga cgtaaaacac tcgctaatgg ggaaataaat
    19921 ccgtaagcca ataaaatacc gaggaaagtc cccaccatcg catgagcgat aagcgccccc
    19981 agctcggcgg caggacgatc ggctgaacct aacgcgtgaa cgacccccat tacagccgca
    20041 acaataccaa acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct
    20101 gcctcgcttt cgtgcgtctc aatctcttca tccatcagag cttcgatttc gaaggtgttc
    20161 atgtgaccgc tgataatcag gcgcagataa tcgacgataa attcaagcat gacgctatcc
    20221 gcaaggatgc gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcgcgt
    20281 tccagcgaaa acatccccat ctgccgcgat ttcgccatca accgataaag cagagccagc
    20341 agatccatat acattgcttt ggtgtatttg gagcgacgaa acagcaacgg cagcgccttc
    20401 agcgtgcctt taatcgcttt gccattattg ccgacgataa acgacccaat ccctgcaccg
    20461 gcaataatca ccagttcagc gggttgatag agtgctccaa ggcttccacc ggtcatcaaa
    20521 taaccgccga aaactgtacc gagaacaacc aggtaaccta ataagataag cacgacatca
    20581 tccttccact gttgaccatg acaggatgtt cagtcgtcag gcgttaacac gcgatagggg
    20641 caaaaaaaag cagcggtacg tcgttaccgc tgctggaatg tttcgccaca ccgtatcagt
    20701 taaacagcct gtactctctg ttcatccagc agttgtggga taatatcggc aggattcagg
    20761 gaaagtttac gtctttttac tgcccgggat ggcggctgac ataagctgca ggcaaagctg
    20821 ccaacaggct ggtgagcatg ggtaataaaa ttgccgccgc agcagttgca gctggaaagt
    20881 tgcagtaatc cactttcaac aaaccgcacc aatgtccagg cacgggttaa tgccagcagc
    20941 ggtccttctt ctgcttgcgg gcactgttca aggtataaac ggtaggcttt gatcaccgca
    21001 tcgacgccat tacacaagcc agttttcagt aaaaactgcc atgcattaca gaacatcgaa
    21061 gcatgaacgt tttgttccca ggtcataaac cagtcggttg agaatggcag catgcctttc
    21121 ggcggtgggc ttccgcgcag ttctttatag agttttatca gacgtccgcg acttaactgt
    21181 gtttcgcttt ccagcatctg caaacgagcg cccagggtga tcaattccat tgccagctga
    21241 atatcccgcg cttcctgaac aatgcttttt tcactcatga tcaggccctt ttcttgcgca
    21301 gcgcttcttc aggctgatta acatcattca gcaagcgtgt tgagagcatg atgccggtat
    21361 gaatttgctg gagatcgtca acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc
    21421 tgtcaaaacg gaagtgacaa accagttgat tggtttctgc cagcttaacc atttgcggaa
    21481 gagtcagtgc cgctaacgtt gtcgccattt cttcatttat gccgagacga aacatagcgg
    21541 acgctttgtc ctgaacaatc agacgctgtg caagtagtaa atatgacaag ttgatgtcat
    21601 aaatgtgttt cagcaactcg gaggtatgca ttattcccac ccagaataac caactttatt
    21661 tttatgcggt ctcaccgcac cccgtgatgt cgccgggaag ccccggtaaa aaataattag
    21721 cattagaata gttgcgataa gctgcaataa gcagacccac ctttttggtt taataagtcc
    21781 ttacaaatag aagggggtct ttacacttat ctaagatttt tcctaaatcg acgcaactgt
    21841 actcgtcact acacgcacat acaacggagg ggggctgcga ttttcaataa tgtgtgatgc
    21901 agatcacaca aaacgctcaa ttacttaaca taaatgtata agtcatactt ttgttttggg
    21961 tgtatttcaa tctgttaaaa aatttttcgc tacactggca agcaaaaatg aaacaggaat
    22021 aatcgaaatg ggatgttgcg cacagttaaa ataactcacc gtaaataatc atctgctata
    22081 aataatcgct ttaatgcaat accagataag ccttttttaa acaaacactt accgcacaac
    22141 aaactaatta actacaaaga aaggcaacaa tcaacctaat gttaatataa tgttttacat
    22201 atattaacca ctt
//
LOCUS       DAZKHK010000089        21395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKHK010000089 DAZKHK010000000
VERSION     DAZKHK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.089"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..663
                     /locus_tag="ACHOS5_003395"
     CDS             112..663
                     /locus_tag="ACHOS5_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFP9778159.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGSAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            complement(841..1833)
                     /gene="rpoS"
                     /locus_tag="ACHOS5_003396"
     CDS             complement(841..1833)
                     /gene="rpoS"
                     /locus_tag="ACHOS5_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP9778160.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1896..2990)
                     /gene="nlpD"
                     /locus_tag="ACHOS5_003397"
     CDS             complement(1896..2990)
                     /gene="nlpD"
                     /locus_tag="ACHOS5_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP9778161.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3175..3801)
                     /gene="pcm"
                     /locus_tag="ACHOS5_003398"
     CDS             complement(3175..3801)
                     /gene="pcm"
                     /locus_tag="ACHOS5_003398"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP9778162.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3795..4556)
                     /gene="surE"
                     /locus_tag="ACHOS5_003399"
     CDS             complement(3795..4556)
                     /gene="surE"
                     /locus_tag="ACHOS5_003399"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP9778163.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4537..5586)
                     /gene="truD"
                     /locus_tag="ACHOS5_003400"
     CDS             complement(4537..5586)
                     /gene="truD"
                     /locus_tag="ACHOS5_003400"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP9778164.1"
                     /translation="MIEFDNLTYLHGKPQSTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSASRSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5583..6062)
                     /gene="ispF"
                     /locus_tag="ACHOS5_003401"
     CDS             complement(5583..6062)
                     /gene="ispF"
                     /locus_tag="ACHOS5_003401"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP9778165.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PRMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(6062..6772)
                     /gene="ispD"
                     /locus_tag="ACHOS5_003402"
     CDS             complement(6062..6772)
                     /gene="ispD"
                     /locus_tag="ACHOS5_003402"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9778166.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6791..7102)
                     /gene="ftsB"
                     /locus_tag="ACHOS5_003403"
     CDS             complement(6791..7102)
                     /gene="ftsB"
                     /locus_tag="ACHOS5_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP9778167.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7295..7618)
                     /locus_tag="ACHOS5_003404"
     CDS             complement(7295..7618)
                     /locus_tag="ACHOS5_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP9778168.1"
                     /translation="MRNSHNITLTNNDGLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7668..8273)
                     /gene="cysC"
                     /locus_tag="ACHOS5_003405"
     CDS             complement(7668..8273)
                     /gene="cysC"
                     /locus_tag="ACHOS5_003405"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP9778169.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8273..9700)
                     /gene="cysN"
                     /locus_tag="ACHOS5_003406"
     CDS             complement(8273..9700)
                     /gene="cysN"
                     /locus_tag="ACHOS5_003406"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP9778170.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9702..10610)
                     /gene="cysD"
                     /locus_tag="ACHOS5_003407"
     CDS             complement(9702..10610)
                     /gene="cysD"
                     /locus_tag="ACHOS5_003407"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP9778171.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10862..11899
                     /gene="iap"
                     /locus_tag="ACHOS5_003408"
     CDS             10862..11899
                     /gene="iap"
                     /locus_tag="ACHOS5_003408"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP9778172.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   11981..12741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=12042..12070
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(12838..13131)
                     /gene="cas2e"
                     /locus_tag="ACHOS5_003409"
     CDS             complement(12838..13131)
                     /gene="cas2e"
                     /locus_tag="ACHOS5_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP9778173.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(13128..14051)
                     /gene="cas1e"
                     /locus_tag="ACHOS5_003410"
     CDS             complement(13128..14051)
                     /gene="cas1e"
                     /locus_tag="ACHOS5_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP9778174.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGHGG"
     gene            complement(14048..14698)
                     /gene="cas6e"
                     /locus_tag="ACHOS5_003411"
     CDS             complement(14048..14698)
                     /gene="cas6e"
                     /locus_tag="ACHOS5_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP9778175.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTRQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(14680..15426)
                     /gene="cas5e"
                     /locus_tag="ACHOS5_003412"
     CDS             complement(14680..15426)
                     /gene="cas5e"
                     /locus_tag="ACHOS5_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP9778176.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLGVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSAPD
                     LLSAIISRRDYYTDAWWMVAVATTPDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYQQYQDSFRELKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            complement(15437..16492)
                     /gene="cas7e"
                     /locus_tag="ACHOS5_003413"
     CDS             complement(15437..16492)
                     /gene="cas7e"
                     /locus_tag="ACHOS5_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP9778177.1"
                     /translation="MTTFIQLHLLTVYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(16507..17043)
                     /gene="casB"
                     /locus_tag="ACHOS5_003414"
                     /gene_synonym="cse2"
     CDS             complement(16507..17043)
                     /gene="casB"
                     /locus_tag="ACHOS5_003414"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP9778178.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTMVNDVCLSEGF
                     RSLLMQTHTLWKIESQEWRFTALALVAAVAANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDELLRQLRRAVKLLNGSVNLISLADDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(17040..18602)
                     /gene="casA"
                     /locus_tag="ACHOS5_003415"
                     /gene_synonym="cse1"
     CDS             complement(17040..18602)
                     /gene="casA"
                     /locus_tag="ACHOS5_003415"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP9778179.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSSSFM
                     QDFEALTGDKVPVASLLPEIPGSQTTKFNKDHFIKWGVTEYLCPHCLALALFSLQLNA
                     PAGGKGYRTGLRGGGPITTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPKKFDD
                     LIFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICDEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(18700..>21395)
                     /gene="cas3"
                     /locus_tag="ACHOS5_003416"
     CDS             complement(18700..>21395)
                     /gene="cas3"
                     /locus_tag="ACHOS5_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP9778180.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVA
                     AMGYLMVKSNCFGLADYFRQLGFADTEQAAQFFAWLLCWHDIGKFARSFQQLYLHPQL
                     KVPEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLKWVMEMWMPMTTGH
                     HGRPPERMDELDNFLPEDKGAARDFLLAIKSLFPLIEIPTFWDDDEGVELIKQLSWYI
                     SATVVLADWTGSSTRFFPRVAQTMDIEDYWQKTLVQAQNALTVFPPKAETAPFTGINT
                     LFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAYRLMSAGKAQGL
                     FFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDSFNESLWSGDLVGS
                     EEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGIEGQQE
                     APFLEKDDYPWLTHVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGK
                     CIAWIRNSVDDAIKIYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKSCS
                     LQRSSQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINSQLKRDGK
                     DERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLRQQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDYLP
                     EKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     EAFRQWCIEQRQDPEMANVILVTDDESCGYSATEGLIGKVG"
BASE COUNT         5132 a         5853 c         5208 g         5202 t
ORIGIN      
        1 tcaaaaaata tgttcatcat ttgatggttt aaataatatc tgcaaggcat aatactccac
       61 atctttaaaa cctgcctcaa aaaatcattc attagtaaaa aggatattta tatgcaaaac
      121 atagctgtgg tttattattc aggatatggt cacactaaac tggttgctga aaaagtggca
      181 caaggcgccc atgcagattt aatttcgatt gataacgaag gaaatatcac tgagcaggag
      241 tgggaaaaac tggacgcagc cgatgccatt atcttcggtg ctcccaccta tatgggcagt
      301 gcgccatggc aattcaagaa atttgctgat gccagttcca aagcgtggtt tacacgtaaa
      361 tggcaggata aagtcttcgg gggtttcacc aacagcgcca gcctgaatgg cgataaacaa
      421 gttacgctga tctatctgca aacgctggct tcacagcatg gcggtctgtg ggtgagttta
      481 ggtcaggctc ctgccaatgt gcttgcgtca accagggaag atgtaaacaa tctgggcggt
      541 tcagtcggtt tgctggtgca atctccagcc gatgcgggtg cagaccaaat ccctacgggc
      601 gatctggata cagccgtgaa atatggtgaa cgcgtggcga caattactgc gcgcttaaaa
      661 tgatgattgc cgaatgtgac gctgacgcgt ctatccggtc ttctacatct aaaaagtgcc
      721 gtctcctggt cagtcagata aagttttaac gctgtacccg ataacagttc aacatgtacc
      781 atgtaccaaa agaaaaaaag ccagcctcgc ttgagactgg cctttctgac agatgcttac
      841 ttactcgcgg aacagcgctt cgatattcag cccctgcgtt tgcaggattt cgcgcaaacg
      901 gcgcaggcct tcaacctgaa tctggcgaac acgttcacgg gtgaggccaa tttcacgacc
      961 tacatcttcc agtgttgccg cttcgtaccc cagcaaaccg aatcgacgtg ccagtacttc
     1021 acgctgtttg gcgttcagct cgaacagcca tttgacgatg ctctgcttca tatcgtcatc
     1081 ttgcgtggta tcttccggac cattctcttt ttcatcggcc aggatgtcca gcaacgcttt
     1141 ttcggaatca ccacccagcg gggtgtctac cgaggtaatg cgctcgttaa gacgaagcat
     1201 acggctgacg tcatcaactg gcttatccag ttgctctgcg atctcttccg cacttggttc
     1261 gtggtccagc ttatgggaca actcacgtgc ggttcgcagg taaacgttca gctcctttac
     1321 gatgtgaatc ggcaaacgaa tagtacgggt ttggttcata atcgcccgtt cgatcgtctg
     1381 gcgaatccac caggttgcgt atgttgagaa gcggaaacca cgttccgggt caaacttctc
     1441 taccgcgcgg atcagcccca ggttgccctc ttcgatcagg tccagcaacg ccagaccacg
     1501 attgccataa cggcgggcaa tttttaccac cagacgcaag ttactctcga tcatccggcg
     1561 gcgagaagcg acatctccac gcagtgcgcg acgcgcaaaa taaacttctt cttcggccgt
     1621 taacagtggt gaataaccaa tctcaccaag gtaaagctga gtcgcgtcca gcacacgctg
     1681 tgtggctccc tgcgataaca gttcctcttc ggccaaatcg ttatcactgg gttcctcttc
     1741 tactaaggcc ttttcgtcaa aaacctcaac tccgttctca tcaaattccg catcttcatt
     1801 taaatcatga actttcagcg tattctgact cataaggtgg ctcctacccg tgatcccttg
     1861 acggaacatt caagcaaaag cctggttccg ccgatttatc gctgcggcaa ataacgcagc
     1921 gggtttacgg atttcccctt gtaacgaatt tcaaaatgca agcgtgttga actggttccg
     1981 gtgctaccca tggttgctat tttttgcccc gccttcactt cttgttgttc ccggaccagc
     2041 attgtgtcgt tatgggcgta ggcactcagg taatcatcat tatgtttgat gataatcaga
     2101 ttaccgtagc cgcgcagcgc gttaccggca taaacaacgc ggccatctgc ggtcgcgata
     2161 attgcctgtc ctttgctgcc tgcgatatca atccccttgt tgcccccctc agaagcgcca
     2221 aaggtttcga tcactttgcc ctcagtcggc cagcgccagg tggagatagg cgtactggtt
     2281 gatgtactgc tgacagtcgg ctcggttgtg cttgctgttg gtaccgttac aggcgctgtg
     2341 accgtggtcg cagttggctt gttgttcggc aacattttgt tagcactctg ttcacccgaa
     2401 gactcagaat acgtaattgt cggttgcgac gcaacagcaa cggtggaatt ttgtgcaggc
     2461 ttgatcacaa ctccttgctc tgctgcgtcg gcctgggtaa tggcatttcc gcctgtgatt
     2521 ggcgtaccgg aagcattacc cacctgtaag gtctgaccga cgttcagcgc gtatggtgcc
     2581 tgaatattgt tgcgctgagc aaggtcacgg aaatcgttgc cagtaatcca ggcgatatag
     2641 aaaagcgtgt cgcctttttt cacggtgtat gtactgccgc tataactgcc tttcggaatg
     2701 ttcccatact gacggttgta gacgattcgt ccgttttcca tctgtaccgg ctgctgagct
     2761 actggctgca ctggctggat ttgcggttgt tgagtagcct gaatttgtgg ctgctgtacc
     2821 ggctgaattt gcggttgctg tgctgtagac gtcgttccca ttttcggcgg cggcgtaatc
     2881 aacataccag aattggtatt tgcaggcgca ttgccattaa cggagctgac cggtgccggt
     2941 ggatttgaag tgtcagaaca gcctgccagc catagcgaaa ccagtgacaa tgccgcaatg
     3001 cggcgaacgg tgaattttgg gcttcccgcg ctcatttatc ccccaggaaa aattggttaa
     3061 taaccagtga cataattacc gtgcaaggca ccatactgaa cactggaaaa gatgttcacg
     3121 atacgctgac ttgcggcaaa ataaccagga aaaatccagg tatttcctca cgttttaagc
     3181 cagctcaccc ttcactaaag ggacaaagcg cacggcctcc acggtatcga taataaattc
     3241 gcctccccga cgacgcaccc gtttcaaata ctggtgctcc tcccctacgg gtaagacgag
     3301 aatcccgcct tcgtccagtt gcgtcattag cgcagttgga atttcaggcg gtgccgccgt
     3361 aacaatgata gcgtcaaacg gcgcgcgtgc ctgccaacct tgccatccat cgccatgacg
     3421 ggttgaaaca ttatgtaaat caagattttt caggcggcga cgcgcctgcc actgcaagcc
     3481 tttaatccgt tcaaccgagc aaacatgctg gacaagatgc gccaggattg cagtttgata
     3541 tcccgaacca gtgccaattt ccagcacccg cgactgcggc gtcagctcga gtaattcggt
     3601 cattcgcgcc accatatacg gctgcgaaat tgtctgcccc tgacctatcg gcaaggcgat
     3661 attgtcccag gctttttgtt caaacgcttc atcaacgaat ttttcacgcg gcacggcggc
     3721 aagtgcattc agcacctgct catcctgaat accttgcgca cgtaattgat ccagaagtgc
     3781 ttgtacgcgt ctgcttacca ttgcgtgcca actcccacgc tgtttaacca gtctgaaacc
     3841 acatcttgcg cgctatgcgc agttaaatcc acatgcagcg gcgtgatgga aacatagccc
     3901 tcatctaccg cagcaaaatc ggtccccgga ccggcatcac atttaccgcc cggcgggcca
     3961 atccagtaca gcgtattgcc gcgcggatct tgctgcggga tcacctgatc tgccggatgt
     4021 cgtgtaccgc agcgcgtcac gcgaatacct ttgatttgat ccaatggtaa atccggaacg
     4081 ttaatattaa gaatacgccc ggtgcgcagc ggctctttac acagtgcgcg caaaattgag
     4141 caggttaccg ccgcggcagt gtcgtaatgt ttatgcccgt caagcgagac ggcaagcgcc
     4201 ggaaaaccta aatgacggcc ttccatcgcg gccgctaccg taccggaata aataacatca
     4261 tcccccagat tcggcccggc gttaattccg gacacaacaa tgtccgggcg cgggcgcatc
     4321 agagcattta cgccaagata gacgcaatcg gtcggggttc ccatttgcac agcaatatca
     4381 ccattttcaa aggtaaacgt gcgcagggag gattccagtg tcagagaatt agaagcgccg
     4441 ctgcggttac gatcgggggc gaccacctga acgtcagcaa actcacgcaa ggctttcgcc
     4501 agcgtttgta taccgggtgc atgtaccccg tcatcattac tcagcaatat gcgcataatc
     4561 acctgttgtg ttgataagtt ccctgacaac gctggttgca aaacttcccg ccggaagcca
     4621 gaaacggatt tctacagtga cgtcatccca ccagttccag cttaattgtt gcggatacag
     4681 cagcatcgct ctgcgcgcgg cttcaacttt ttcgcgcacc agtaaggttt gtaattcagt
     4741 ttcttcggcg acagctgctt gttcgaatgc cagcgcttca cgctgagttc cccattcgcc
     4801 actgcccggc aatgcggcgg ttatcatcag ctctttatcg ttgacgcgac gctgtaattc
     4861 cgccagttct tcggtggttg caacaaacca gctaccacgt ccggctaatt gtagcgcatc
     4921 gccgtcaaca acttgattaa cgtctgcttt tttgaggcgc tcagcaacaa tctgattaaa
     4981 caacgcactg cgggatgccg acaaccaaaa actccgttta ttgcgatcgc gcaccggagt
     5041 attggtttgc gcccagcgca gcgccccctg caagttgcta ccgccaatcc caaaacgttg
     5101 ggcaccgaag tagttcggta caccttttac gcaaatatcg atcagacgtt gttcaacgtc
     5161 atcgcgattg ctcacttcgc gtagtaccaa ggtaaaggcg ttacctttca gcgcccctaa
     5221 acgcagcttg cgcttgtgcc gcgcatactc cagcacctgg cagccttcca gttgaaaggc
     5281 gctcagatcg ggcatctctt tgcccggcac gcgagcgcat aaccactgtt ccgtaacagc
     5341 atgtttatct ttttgcccag cgaagctgac ttcacgggca tgaattttca ggaatttcgc
     5401 cagtgcatcc gccacaaaac gggtattgca gccgtttttg aggattctaa ccagaatatg
     5461 ctcaccttca ccatcaggct caaagcccaa atcttccacc accacaaagt cttccggatt
     5521 ggctttcagc agcccggtgc tttgcggttt accgtggagg taagtgagat tatcaaactc
     5581 aatcattttg ttgccttaat gagtagcgcc accgcttcac aggcaatccc ttccccacgt
     5641 ccggtaaatc ccagtttttc cgtagtagtg gctttcacgt taacatcatc catatggcag
     5701 ccgagatctt cggcaataaa cacgcgcatt tgtggaatgt gcggcaacat cctcggtgcc
     5761 tgagcgatga tagtgacatc gacgttgccg agggcataac ctttcgcctg aatacgacgc
     5821 caggcttcgc gcaacagctc gcggctgtcg gcacctttaa atgccggatc ggtatccggg
     5881 aacagcttgc ctatatctcc cagcgccgcc gcgccaagca atgcatcggt caacgcatgg
     5941 agcgccacgt cgccatcaga atgcgccagc agcccttttt cgtaaggaat gcgtacgcca
     6001 ccaatgataa ttgggccttc accgccaaag gcgtgtacgt caaaaccgtg tccaattcgc
     6061 attatgtatt ctcctgatgg atggttcggg tgaggtaaaa ttcggccagc gccaaatcct
     6121 ccgggcgcgt taccttgatg ttatccgcgc ggccttcaac aagttgtgga tgaaaaccgc
     6181 aatattccag cgccgaggcc tcatcggtaa tggtagcgcc ttcatttaga gcgcgcgtca
     6241 ggcagtcatg taacagctca cgagggaaaa attgcggcgt cagcgcgtgc cataagccgt
     6301 tgcgatcaac ggtatgagca atggcatttt tgcccggttc ggcacgtttc atcgtatcgc
     6361 gcaccggcgc agcgaggatc cctcccgtgc ggctggtttc gctcaacgcc aacaatcgcg
     6421 cgaggtcatc ctgatgcaga caaggacgag cggcgtcatg caccaatacc cactgcgcgt
     6481 cgccagcggc tttcagacct gccagcacgg aatcggcacg ctcttcacca ccatcaacaa
     6541 ccgtgattcg tggatgattc gccagaggaa gttgtgcaaa gcggctatcg ccaggactta
     6601 tggcaatgac gacacgtttc accctgggat gcgccagcag cgcatgcacc gagtgttcaa
     6661 gaatggtttg attaccgatt gagagatatt gcttaggaca ttccgtttgc attcggcggc
     6721 caaatccggc cgccggaacc acggcgcaaa catccaaatg agtggttgcc atgttaattc
     6781 ccgggctgat ttatcgattg ttttgccccg cagactgtgc gcgctttgac gcgtcaggca
     6841 ccagacgata aaacgtttcg cccggcctgg tcatgctgag ttcattacgc gcacgctctt
     6901 cgagcgcctc ctggccgcca ttgagatcgt caatttcggc aaaaagctga tcgtttcgcg
     6961 ctttaagttt cgcgtttgta gcttgctgtg ccgccacatc attattgacg cgagtatagt
     7021 catgtatacc gttcttaccg aaccacagcg aatactgtag ccagaccaga atagccagca
     7081 acagcagcgt tagtttaccc atcctgcccc ctgaaaaacg gcatcttcat cccatgcatc
     7141 cgaagacgac tctacatcct ctgttgggga taccgcgaca acgcgggcaa atgtaccaca
     7201 tttgtccatt gttacgtata cccagggcgt gcagaacata atctcattat tagttacgga
     7261 ttgaattatg aacagaatag agaggaaaga acaattaacc cagtagccac ataaacagcg
     7321 caccaaacat aatgccaacc gtcgccagag tgaaaacaat actgtagcgt agctttccgt
     7381 ccatcaatga atgcagtgca atccccacca ccactgcgac gggcatcagc gccagaaaga
     7441 aaggccaggt gtagataaaa aagagcagcg tgttagagcc ataaatcaac atcggaattg
     7501 ccaacgcgca cagccacgat aaaaaaccaa ccacagcgcc tggcagcgac catgtggtgt
     7561 cttcaacctc agtaaggccg tcgttatttg ttaatgtaat gttgtggctg ttacgcatat
     7621 ctgatcctgt tactttgacg aaccgggcat gaaaacccgg tggtgtctca ggatctgata
     7681 atatcgttct gtctcaacag atctaataat tgctgtacca aatttgttac taattgttca
     7741 ccattgagat gaatttctgc cgattcaggc gcttcgtaaa cggaatctat tcccgtaaag
     7801 ttgcgcagtt caccggcacg cgctttctta tataaacctt ttggatcgcg ggcttcgcaa
     7861 atcgccagcg gcgtatcgac aaacacttcg ataaagcgcc cttctcctac gcgttcgcga
     7921 accatctggc gttcggcgcg gtgtggcgag ataaatgcgg tcagcaccac cagtccggct
     7981 tcaaccatca aattcgccac ttcaccgacg cgacggatat tctctttacg gtcggcatcg
     8041 ctaaaaccga gatcgctgca taatccgtgg cgaacgttgt cgccatccag cagatacgta
     8101 ctgacgccaa gtttatgtaa cgcctcctcc agcgcccccg cgacggttga tttaccggaa
     8161 ccagagaggc cggtaaacca cagcacaaca ccacgatgac cgtggtgctg ttcgcgttgt
     8221 tgcggagtta ctgggtggct atgccagacg acgttttcgt catgcagagc cattatttat
     8281 ctcccagtaa atcgcgcgca ccccaatgcg ggaaatggcg gcgaaccagg gcattcaatt
     8341 ccagttcgaa tgcactgaat tcagatggcg cagcagttgc ctggctaact ggttcgtgca
     8401 ccataccggc acctacggtc acattactca ggcgatcgat aaaaatcagc ccaccggtaa
     8461 ccgggttttg ctgataacga tctaacacca gtggctcgtc aaaggtgaga tctaccaggc
     8521 cgatgccgtt cagcggcagg ttttcaactt cgcgttgggt aaggttattg atatcaacct
     8581 gataacgaat gccatcaaca cgagcacgcg tcttcttacc ggcaattttg atgtcataac
     8641 tctggcccgg ggaaagcggc tgttccgcca tccataccac atccaccgac gcgctctgca
     8701 cagccggtaa cgcttcgtct gccgccagca gcagatcgcc acggctgatg tcgatctcat
     8761 ccgtcagcac cagggtgatc gcttctccgg caaaggcttc ttcgcgatcg ccatcaaaag
     8821 tcacgatccg cgcgacgttt gattccacac cagagggcag cactttgaca cgttgcccga
     8881 cttccacgcg accggatgcc agcgttccgg cgtagccacg aaaatcgaga ttcggacggt
     8941 taacgtactg caccgggaag cgcattggct gagcatccac cactcgctgg atctccacgg
     9001 tttccagcac ttctagcagt gtcggaccgc tgtaccacgg catactttca ctttgcgaag
     9061 ccacgttgtc gccttccagt gcggagagcg gcacaaagcg gatatccaga ttacccggca
     9121 gctgcccggc aaaagtcaga taatcttcac gaatacgggt gaacgtctct tcactgtaat
     9181 ccaccagatc cattttgttg atcgccacga ccagatgttt gatccccaac agtgtggaga
     9241 taaaactgtg acgacgggtt tgatcgagca cgcctttacg ggcatcgatc agtaagatcg
     9301 ccagttcaca tgtcgatgcg ccagtcgcca tattgcgggt gtactgctcg tgccctgggg
     9361 tgtcggcgat aataaattta cgcttctcgg tagagaaata gcggtaggcc acatcaatag
     9421 tgatgccctg ttcgcgctca gcttgcaggc cgtccaccag cagtgccaga tccagctttt
     9481 cgccctgggt gccgtgacgc ttactgtcgt tatgcagcga tgagagctga tcttcgtaga
     9541 tttggcgggt atcgtgcagc aggcgaccaa tcagggtact tttgccgtca tcgacgctac
     9601 cacaggtcag aaaacgcagc aggcttttat gttgttgcgc aatcatccag gcttcgacgc
     9661 cgccttcatt ggcgatttgt tgtgcaagtg cggtgttcat cttaaaaata cccctgacgt
     9721 tttttcagct ccatagaccc cgcctggtcg cggtcaatta cgcggccctg acgttcactg
     9781 gtggtggaaa ccagcatctc ttcgatgatc tccggcagtg tttgcgcatt tgactccacc
     9841 gcgccggtca gcggccaaca gcccagtgtg cggaaacgca ccatccgttt tttaatcact
     9901 tcgcccggtt gcaggtcgat acggttgtca tcaatcatca tcaacatacc gtcgcgttcc
     9961 agaaccggac gttccgcagc aagatacagc ggaacaatgt cgatattttc cagccagatg
    10021 tattgccaga tatcctgctc ggtccagtta gaaagcggga agacgcggat gctttcgcct
    10081 ttgttaattt gcccgttgta gttgtgccac agctccgggc gctgattttt cggatcccag
    10141 cgatggaagc ggtcacggaa agagtagatt cgctctttag cgcgggattt ctcttcgtca
    10201 cggcgcgcac caccgaaggc ggcatcaaaa ccgtatttgt tcagcgcctg tttcaggcct
    10261 tcagttttca taatatcggt atgtttcgcg ctgccgtgta cgaatggatt aatccccatc
    10321 gccacgcctt ccgggttttt atgcaccagc agctcgcagc cgtaggcttt cgccgtacga
    10381 tcgcggaact catacatctc gcggaatttc cagccggtat cgacatgcag caacgggaaa
    10441 ggcagcgtac ctggataaaa cgccttgcgc gccagatgca gcatgacgct ggaatcttta
    10501 ccgatagagt agagcatcac cggatttgag aattctgcag ccacctcgcg aataatgtgg
    10561 atgctttccg cctccagttg ccgcaggtgg gtaagtcgtt tttgatccat aaccgttcct
    10621 ttgcaatacc gctattttct tgccatcaga tgtttcgact atagggagcg taagagaacg
    10681 aatgaaatta ccaattagaa tgagtagttc cttaacggaa taacgatttg gcaaagctaa
    10741 tatcaaaaag tgcttaaggc accggatttc gggcgtttag gaagatttga aattgtttta
    10801 gcgcagcggc agtttcatac tatggcggta aaaaaatttg catggtattt aaggactcac
    10861 tatgttttcc gcattgcgcc accgtaccgc tgccctggcg ctcggcgtat gctttattct
    10921 ccccgtacac gcctcgtcac ctaaacctgg cgattttgct aatacccagg cacgacatat
    10981 tgctactttc tttccgggac gcatgaccgg aactcctgca gaaatgttat ctgccgatta
    11041 tattcgccaa cagtttcagc aaatgggtta tcgcagtgat attcggacat ttaatagtcg
    11101 gtatatttat accgcccgcg ataatcgcaa gagctggcat aacgtgacgg gaagtacggt
    11161 gattgccgct catgaaggca aagcgccgca gcagatcatc attatggcgc atctggatac
    11221 ttacgccccg ctgagcgatg ctgacgccga tgccaatctc ggcgggctaa cgttacaagg
    11281 aatggatgat aacgccgcag gtttaggtgt catgctggaa ctggctgaac gcctgaaaaa
    11341 tacgcctacc gagtatggta ttcgatttgt ggcgaccagc ggcgaagagg aaggaaaatt
    11401 aggcgctgag aatttactca agcggatgag tgacaccgaa aagaaaaata cgctgctggt
    11461 gattaatctc gataacttaa ttgttggcga taagctgtat ttcaacagtg gcgtaaaaac
    11521 acctgaagca gtaaggaaat taacgcgcga ccgggcgctg gcaattgccc gcagccatgg
    11581 aattgccgcc accacaaatc caggtttgaa taaaaactat ccgaaaggca ccgggtgttg
    11641 taatgacgca gaaatattcg acaaagccgg tattgctgta ctttcggtgg aagcgactaa
    11701 ctggaatctt ggcaataagg atggttatca gcaacgcgca aaaacagccg cattccctgc
    11761 gggaaatagc tggcatgacg taagactgga taatcaacag catattgata aggcacttcc
    11821 tggaagaata gaacgtcgct gccgtgatgt tatgcggata atgctaccgc tggtgaagga
    11881 gctggcgaag gcgtcttgat gggtatgaaa atgggagctg ggagttctac cgcaggggcg
    11941 gggaactcca agtgatatcc atcatcgcat ccagtgcgtc cggtttatcc ccgctgacgc
    12001 ggggaacaca catgaatgtc ggttcagacc gtgtttttac ccggtttatc cccgctggcg
    12061 cggggaacac atatcagcgt tatcaaccgc gatttcagtt tccggtttat ccccgctggc
    12121 gcggggaaca caatttattc agccaggtga ccagctcgcc ggacggttta tccccgctgg
    12181 cgcggggaac actacgtacc agcgatttct gtctgaagtt gaggcggttt atccccgctg
    12241 gcgcggggaa cacctggaga tagagcgata tatcccggtc gctggcggtt tatccccgct
    12301 ggcgcgggga acactcaaca accgggcggc cccagccacc aacgcccggt ttatccccgc
    12361 tggcgcgggg aacacttacg tccgcacaca aggatgcgga aaaaccacgg tttatccccg
    12421 ctggcgcggg gaacacgaaa gttttgtaat tcagcgtcat cgttttaccg gtttatcccc
    12481 gctggcgcgg ggaacaccac taccagtgat ataaccggcg tatcccgcgc ggtttatccc
    12541 cgctggcgcg gggaacacca aaacgcctcc cacggtgcac ggaaatactt cggtttatcc
    12601 ccgctggcgc ggggaacaca gcgataactg ctgatttagg tgtattttcg gcggtttatc
    12661 cccgctggcg cggggaacac gaaattcgcg ccgcatatac tgataactac ggcggtttat
    12721 ccccgctggc gcggggaaca cactaagcat acataactgt ttttaaacaa atttattccg
    12781 catcaacaat ctaccaacta aattaaaaca tttccttatt tttaaagaac ggataactta
    12841 ttgattatca acaggaagaa aagaaaccaa acgcaaccca tccaaatcca ccggaatacg
    12901 tctattttca ccccaggtct gaaattcaaa acccgactcg gtattggtcg cccaggccat
    12961 taccacattt ccgcaacctg ccagttgaga gatttgctgc cagatcatct cccgaatacg
    13021 tttggatgta tctccgacat aaacaccggc acgcacttca agtagccaga ttgcgagccg
    13081 tccacgtaag cgcggaggga cattttctgt aacaacgacg accatactca tccgccgtgc
    13141 ccccggtgac cgctatcacc cagcgtttca ggttcaggga tggcaggcgg taacatatcc
    13201 ggcgcaggtt gtggtggttc aatttcacct gcggcaagca cttcctcgat taacggaatt
    13261 aatttgcccg ttaacttact gctacgaaaa atatcacgac aagccaacct gacttcttta
    13321 tctggttctg cgggttgcct cgctgctatt tcaaatgcct ttggcacaac cgaatcaaat
    13381 ttaatgatat cggctatgtc ataaacaaat gaaagcggtt tgccactatg gataaatcca
    13441 atagcgggcg catatcccgc ggctaatact gccgcttcag aaataccgta cagacatgat
    13501 gtggcagcac tgatgcagcg attcacaaca tcgccttttt cccagtcttt aggatcgtat
    13561 ttgcgaccat tccatttcac accatattgt ttcgccagta atgcatacgt ctggcgaacg
    13621 cgggatccct caattccacg tagctgctcc actgaacggc gagctggcgg tggctcacga
    13681 aaacgtaatt catacatttt gcgcaccacc ttcagacgta gatcttccgt taaagccagc
    13741 tttgcctggt agagtaactt atcagcacgc gccccaccgg gttgtccgga agagtagacg
    13801 cgaacacccg cttcaccgac ccagaccagt agcgtcccca ccgtggcggc cagatgcacc
    13861 gccgcgtggg aaactctcgt tcccggttcg agcataatgc aggcgaccga tcccaccgga
    13921 atgtgcgtgc ggatcccggt tttgtcgatc agcacgaaag cgccgtccag tacgtcgatt
    13981 tgaccgtact ggaggaagat catggaagtg cgatctttta atgggatcgg gctcagcggt
    14041 acaaacgtca cgcctctgct ccgggtttga tcagcatcag accacaaccg aacgcccggc
    14101 ttttgccata cccctgactg agacgttgca gaaataaccc aggatcggtg accgtaagca
    14161 tccccgtata atccacgcta ctgaactgga tcagttgccg ggagttttcc cgccgcagtt
    14221 gctgttgtcg gtaggcatca accgaggtat ccagtagtgt aaaaccactt ctttccccct
    14281 gcgctgccag ccagtccagc gccgcctgtt gttgatgcaa ccagacatca cttccttccg
    14341 cctgtcccct cacctgccgt ttcgcctcca tcagcagatc gtggcgcttg cccgctttac
    14401 agattgttgg attcgcccgc aggttgaaac aaagttgttg tctggtacgc agctcgggca
    14461 caaatgaccg gcattcgatg gtgaacgttt cactttccgc cgggcgttcc tgcgacagga
    14521 caaaaaagcg aaacgccccc tggagttctt cccgacgata aagaaattgc ctttctttgc
    14581 cgccaggaaa aaggtcccac agccactgat gcattacata ttcaccgcga tccaccaaat
    14641 gcagcaactg cgcaggcgaa agctggccgg tatgtaaggt tattcttgag aggtacatgg
    14701 ttcctccttg ctgagccacg gcccctgatt gatggtgcgt tccccaaaca gccactgctg
    14761 acgatttaaa ggaacatccc ggcgacgtaa tattttgctc gcgaccaggc cgtcgtgttc
    14821 cccttcccac cagcattcat cctgaagttt cgggagtgag actttcagtt cgcggaaact
    14881 atcctgatac tgttggtatg cgttacgtaa gacatcagac gcgttgcctt cgagcagtaa
    14941 cggcgcaagt ggtaacgcca gcggatgact ttttcgcccc agataaagcg gaaaaaccgg
    15001 atgacgtaaa ccgtcctgca actgttcaag gctgtaaggc gcatcggggg ttgttgccac
    15061 cgccaccatc caccaggcat cggtgtagta gtcgcgccgg gagataatcg cgctcagaag
    15121 atcaggggcg ctcaactctt cgcgacggct gaaataacgc gctttacgca cctcttttgg
    15181 catctggacc gtgtgataat cccgtgccca gcgcgggtta cggctggcgc aaacccccag
    15241 tgaatagtgg cggttaaacg cgtttaatcg ttcggtatca tcacgccgaa tccctacccc
    15301 ggcagccagc agccccagca atgctgagcg cgaaggcagt tcatgggtat gacgcacttc
    15361 gccgggggca tcgacgcccc aggatgccat tggcccatga agctgaaaaa tcaaatattg
    15421 gctcattagc cgccccttac gcgcagataa agtccagcac gtccttcatg cttccctgtt
    15481 tgttcatcac gtcaaagctt gcgcattcgg tcttctgttc atagaccgta ttcatatttt
    15541 cgcgaagcgt tgtaatacgc tgcaccgcca catctaactg gcgggtgcca ttaatgggtt
    15601 catagaaagc cgccgccaga gagcgtggtt gttcagtgcc tttttctgcc agcgcccagg
    15661 aggcgtaggc acggctggca aagctgttct gtttgccggt tggggagact ttaagtgcgg
    15721 cttccgtaaa ggcgcgcaag gtctgattag ctaacgcttc gtcaccgccg aggttttcga
    15781 ccagcagatc tttatcgatg cagatatagg tgtagaacag cgcagaaccg aatccggttt
    15841 caccaagatg cccggcaccg gcatcttcag aagcctggcg caaatcatca acggcggtga
    15901 aaaaatcatc ttcgacaatc gtttcactga caccaaatgc atgcgcgacc tggcaggcgg
    15961 cttcaacatt aaattcgggt ttattcgcca gcatacgacc aaacatagcg atatctaccg
    16021 ccatgcgatc tttacgtaac aaagcgagat cttcctcttt tggcgcgcgc ttttcttcgg
    16081 ccagttgatg ggccagcgct tttacggcgt caaattctgc cgggctgata tggactagct
    16141 gttcagtttc ggcgttagtg agcggatctt ttggtttttt gtcgttttta gctttcccaa
    16201 gataatccgc aatttttgcc gcccattcga tggctttttt ctcttcgatg cctttctcaa
    16261 tcaggatagt tgccgcctca cgcgcaatac gcccactgcg aataccaata tggcccgcca
    16321 gtgcctgttc aaaaagtgca gaagtgcgcc acgcacgttt cagactttgt gaggaaacgc
    16381 gcagacgcgt tgctccaccc agtaccacag tttttggcgc accggtgtca tcacggttaa
    16441 ggttggcagc agggtaaacg gttaacaaat gaagctgaat aaatgtcgtc atgagatagt
    16501 cctttattaa ttttgttcgt tatcggcgtc gccagcctgg taatattcca gcgcccagcg
    16561 gatacggata aattctgttg gccgctgctg gcggcggtga tgattgagca gatcgtcgct
    16621 ctcctggcac cagcggaaaa tatcgtctgc caaagagata agattgacgg aaccatttaa
    16681 taatttgacc gcgcgacgta actggcgcag taattcatcc ggcgttttga ctgccgacag
    16741 gcgagtaaaa cgcccctcag acattaccgc cgccaactgc gcggcaaaag gctgccgttc
    16801 atcaatggct tttacattgg cggcaacggc ggcaaccagc gccagtgcgg taaaacgcca
    16861 ctcctgtgat tcaattttcc acaacgtgtg ggtctgcatc agcagagaac gaaacccttc
    16921 tgacagacaa acatcattaa ccattgtgct acgccgcaga ctggcacgat tgccgcgttt
    16981 ttcctgcaac tcttcgtgcc atttacgcag cgtggctttg tgctcctctt ttacaatact
    17041 cattcagcag cctcctgctt tttctccctg gcggctttag cactttgctt ctccgccgat
    17101 gttgtaaaat atttcttgcg cgcggtcatg acgcgtttca aatcaacggg ctcataagga
    17161 ttagtgaata cacgctcgtc aaaatcctga cgcgcgaata accaaatttc cttttgccat
    17221 ttgccgagta attcatccgc atcctgacct tcttcaattt ggcgcactaa cctcaggaag
    17281 cgatgctgag ttttgttcca gaagtcgata tccacaaaac tgaaatcacc ccttgcacct
    17341 tttggatcgg agaaccatgc ttctttcaat gcactccgta acagactcag aatccgtgaa
    17401 gccgtttgcg cagccagccg cagcttcggt atctggcctt cttttttatt gagcagtagc
    17461 gggaaatggt gttcgtacca acagcgcgct ttcatgttgt caaaatcata accaaatccc
    17521 cataggccca cttttgcctg tttcagactg ctggcattaa agagtttcac caccagtgca
    17581 ggaagttccg tattgttttc tgatttaccc gtttcgataa ggcctaacca gtcgcgccag
    17641 attaaaccgc ccggttgtgg tttaacggag taaaactcgc caccctcttt aagtggtaca
    17701 cggtaaggcg ttaagggatg ctgccacatg gcataattgg caccataatt tttggtcgtc
    17761 atcaggctca ggagcgcgtc gttctgctcg tcgcaaatat cgcagttgcc gactgtcgtg
    17821 gtattaaaat caatacgtat acgccgcggc attccccagt acgcctggag tttattgacc
    17881 tgatcatggg ttaccaccgc accggccagt tcgctggtac gcgtcgggcc tagccagggg
    17941 aaaatcagat cgtcaaattt tttgggtagc ggtaagtcgg cttcatcctg cggcatcacg
    18001 ttgagccaca gtttgcgcca caagggtgtt tgttgattgc cctgatattc ctgtaattca
    18061 atgagagtcg ttatcggccc accgccgcgt aaaccggtgc gatagccttt gccgcccgcg
    18121 ggtgcattca actgtaaaga gaacaaggcc aatgctaagc aatgggggca cagatattcc
    18181 gttacacccc acttgataaa atggtcttta ttaaacttcg tcgtttgaga gccgggaatc
    18241 tcaggcagta gcgaagcgac cggaacttta tctcccgtga gcgcctcaaa atcctgcata
    18301 aatgaagatg aatcaggccc aaactggaaa gcatgttcta aagacagcaa tgcttctcgt
    18361 agcttttcag cttccagtcc gtcttcccag atatcatccc aacgacgatg atcttttggc
    18421 gcgaagctgg tttgtagtag tcccagcaaa aactgccagg ccgcaccttg taaatctgct
    18481 cgcggtgcgg cgatatcgac aacattttca tcagccagat cgactggtgc cagtttgcct
    18541 gtcgttccgt ctttaaaacg aacgggcaac cacggggttg tcagaagtga aaacgagttc
    18601 atacgcatcc tctcgtaaaa aagaccattc caggtcggtt gtccatcctt ccatcctttt
    18661 ctgtatatca actattcaga gagtcctgtc gcagacatct caaccaacct tgccaatcaa
    18721 tccttccgta gccgaatacc cgcaactttc gtcatcggta actaaaatca cgtttgccat
    18781 ctccggatct tgccgctgtt caatgcacca ctgcctgaac gcttcccctt ccagtaaaga
    18841 aaactcatcc cgatgttttt tccaccagct tcgacgcact ctgacaacgc tcatttccca
    18901 tgcgtgagca ccggtggcat aaggcttcac cacaccggca atacaggtag ccagccacag
    18961 ggaaacagat tcctcagcca gacgtgtcga cagcttttcc ggaaggtaat cgttgatatt
    19021 ggcggcatag ccaggcttga agttcaggac aaacttttta gccattgcgc gatcgcagta
    19081 atatttgccc acttgctcct gctcgctgcg ggcaaatcct tccggcatta ccacgtcctc
    19141 accgtagact gattcaataa gaaggcgggc tgcgtgtggc atttgaatag cgccttgctg
    19201 acgcagtaca cgctgcgtca gccagattcg tccatgatcg ggatagacat atgcactgtt
    19261 acgcatggca ctgccgaacc attcgtcacc aggagcgtcg tcccagacgg gggccagaat
    19321 cagcaattca ggaggggaac gctcgtcttt tccgtcacgc tttaactgac tattaatatc
    19381 gcggatatgc ctctgtaatc gtcctgctcg ctgaatcagc aaatcaatgg gggcaaggtc
    19441 ggaaatcatt tcgtccaggt caatatccac actttgttca attacttgtg tacaaacaat
    19501 gacttgcgat gatctctgta aactacaact tttaccgaag cgtgccagcg tttccgtttc
    19561 aattcgctgg cgatcgctaa aagcaaagcg gctatgaaac agagaaaggc tggaagcagg
    19621 aatgacgccg cgggcaagca actgacgata aatcttaata gcgtcatcga cagaattccg
    19681 gatccaggcg atgcatttcc cctgacttac tgccgattcg atacgcgcaa tacattcttg
    19741 ttcgctatga agccaaccca cgctcacgct acgctcaacg tctttgcgcg tcgctacccg
    19801 gtgtgaatgc acatcggatt tcgtaacgtg cgtcagccag gggtaatcat ccttttccag
    19861 gaacggagct tcttgctggc cctctatacc acgcgcaaag gcagcaacga gtttgtcgcg
    19921 ctgctgttga gataacgtgg cagaaagcaa aatgacgctg tttccgccac gcgcctgccg
    19981 ctcgatcagc ccttcaagaa tgcatgacat gtaagcgtca caggcgtgga tctcatcagc
    20041 cagcaggatt ttattactca gccccagaag ccgcagatta ttatgtttga acggcatcac
    20101 cgccatcatc gcctgatcca aagtgccgac gccaatttca gccagcaacg ccttcttgtt
    20161 actgtcggca aaccaggccg cacatccctg gctgaaagct tgttcatccg gttcttctga
    20221 cccgactaaa tcaccggacc agagtgattc attaaagctg tccattaatg tgcgggcact
    20281 gtgtgccagc accaggctgg ggcgggactc tggcgaatag aaagcaagcc aggttttgac
    20341 caacctatcg tacatggcat tagccgttgc cattgttggc aggccaaaaa acaaaccctg
    20401 tgctttcccc gcagacatca acctgtacgc caggataagc gccgcttctg ttttacctgc
    20461 gccggttaca tcctccagaa taaataactg tggtcccggc tggctgatat ccagatccag
    20521 caccttttgc tgtagcggtg tcggattctc aataaaagga aacagcgtat taattccggt
    20581 gaaaggcgcg gtttctgctt ttggaggaaa gacggttaag gcgttttgag cctgaactaa
    20641 agttttctgc cagtaatctt caatatccat tgtctgtgcg acgcgtggaa aaaatcgcgt
    20701 tgacgatccc gtccagtctg caagtacgac ggttgcagag atataccagg aaagttgttt
    20761 tataagttca acgccctcgt catcatccca gaatgtgggg atctctatga gcggaaacag
    20821 tgacttgatt gcaaggagaa aatctcgtgc ggcacctttg tcttcaggca gaaaattatc
    20881 cagttcatcc atacgctcag ggggccgacc atgatgcccg gtagtcatgg gcatccacat
    20941 ctccatcacc catttaagtt tgcgcggaga gagtgaagat gaaggaagca actcctgaca
    21001 ttcacttaaa taatggttcc acaaccagta gcccagcgtt gaatgagaga tcttttcgta
    21061 attctttctt gcgccttccg ggaccttgag ttgtgggtgc aggtaaagtt gctgaaaaga
    21121 gcgggcaaat tttccaatat cgtgccagca cagcagccag gcgaaaaatt gtgctgcctg
    21181 ctccgtgtca gcgaaaccta attgacgaaa atagtcagcc agtccgaagc aattgctttt
    21241 aaccattaaa tagcccatcg cggctacatc cagcgaatgc cagcaaagaa ggtgatagtc
    21301 gtcgcccccg tctttctcgc cgcgtcgggt ttttccccag aaatcaaaaa aagtcacaat
    21361 atttttatcc ttcagtaaac ttaaaggata tttac
//
LOCUS       DAZKHK010000090        21328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKHK010000090 DAZKHK010000000
VERSION     DAZKHK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.090"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..368
                     /locus_tag="ACHOS5_003417"
     CDS             <1..368
                     /locus_tag="ACHOS5_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9778181.1"
                     /translation="KDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRISPI
                     NYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKN
                     CFAEQEILSEFPQPVSLAG"
     gene            complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHOS5_003418"
     CDS             complement(411..1562)
                     /gene="ydiO"
                     /locus_tag="ACHOS5_003418"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP9778182.1"
                     /translation="MDFSLTEEQELLLTSIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNSISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLKTGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHOS5_003419"
     CDS             complement(1576..3171)
                     /gene="ydiF"
                     /locus_tag="ACHOS5_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP9778183.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3318..4076)
                     /gene="aroD"
                     /locus_tag="ACHOS5_003420"
     CDS             complement(3318..4076)
                     /gene="aroD"
                     /locus_tag="ACHOS5_003420"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9778184.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKQASAPGQISVNDLRTVLTILHQA"
     gene            complement(4107..4973)
                     /gene="ydiB"
                     /locus_tag="ACHOS5_003421"
     CDS             complement(4107..4973)
                     /gene="ydiB"
                     /locus_tag="ACHOS5_003421"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP9778185.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4985..6250)
                     /gene="ydiN"
                     /locus_tag="ACHOS5_003422"
     CDS             complement(4985..6250)
                     /gene="ydiN"
                     /locus_tag="ACHOS5_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9778186.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(6477..7691)
                     /gene="ydiM"
                     /locus_tag="ACHOS5_003423"
     CDS             complement(6477..7691)
                     /gene="ydiM"
                     /locus_tag="ACHOS5_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9778187.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(7791..8147)
                     /gene="ydiL"
                     /locus_tag="ACHOS5_003424"
     CDS             complement(7791..8147)
                     /gene="ydiL"
                     /locus_tag="ACHOS5_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP9778188.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8283..8390)
                     /gene="rprA"
                     /locus_tag="ACHOS5_003425"
     ncRNA           complement(8283..8390)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOS5_003425"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8576..9688)
                     /gene="ydiK"
                     /locus_tag="ACHOS5_003426"
     CDS             complement(8576..9688)
                     /gene="ydiK"
                     /locus_tag="ACHOS5_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP9778189.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            9902..12958
                     /gene="ydiJ"
                     /locus_tag="ACHOS5_003427"
     CDS             9902..12958
                     /gene="ydiJ"
                     /locus_tag="ACHOS5_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP9778190.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTIIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMIDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKALHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            12955..13365
                     /gene="menI"
                     /locus_tag="ACHOS5_003428"
     CDS             12955..13365
                     /gene="menI"
                     /locus_tag="ACHOS5_003428"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP9778191.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13465..13653
                     /gene="ydiH"
                     /locus_tag="ACHOS5_003429"
     CDS             13465..13653
                     /gene="ydiH"
                     /locus_tag="ACHOS5_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP9778192.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14202..14570
                     /gene="sufA"
                     /locus_tag="ACHOS5_003430"
     CDS             14202..14570
                     /gene="sufA"
                     /locus_tag="ACHOS5_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP9778193.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14579..16066
                     /gene="sufB"
                     /locus_tag="ACHOS5_003431"
     CDS             14579..16066
                     /gene="sufB"
                     /locus_tag="ACHOS5_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9778194.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYRKKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16076..16822
                     /gene="sufC"
                     /locus_tag="ACHOS5_003432"
     CDS             16076..16822
                     /gene="sufC"
                     /locus_tag="ACHOS5_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9778195.1"
                     /translation="MLSIKGLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16797..18068
                     /gene="sufD"
                     /locus_tag="ACHOS5_003433"
     CDS             16797..18068
                     /gene="sufD"
                     /locus_tag="ACHOS5_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9778196.1"
                     /translation="MAGLPNSSNALQQWNHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEIAPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVLARIGQRLPGGAR"
     gene            18065..19285
                     /gene="sufS"
                     /locus_tag="ACHOS5_003434"
     CDS             18065..19285
                     /gene="sufS"
                     /locus_tag="ACHOS5_003434"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP9778197.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19298..19714
                     /gene="sufE"
                     /locus_tag="ACHOS5_003435"
     CDS             19298..19714
                     /gene="sufE"
                     /locus_tag="ACHOS5_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP9778198.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19863..20867
                     /gene="ldtE"
                     /locus_tag="ACHOS5_003436"
     CDS             19863..20867
                     /gene="ldtE"
                     /locus_tag="ACHOS5_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP9778199.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(20930..21166)
                     /gene="lpp"
                     /locus_tag="ACHOS5_003437"
     CDS             complement(20930..21166)
                     /gene="lpp"
                     /locus_tag="ACHOS5_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP9778200.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5552 a         5168 c         5384 g         5224 t
ORIGIN      
        1 taaaagatat tcttttctat ctgaataata actatcgcga aaaaatcact ttagagcagt
       61 tatcgaaaaa atttcgtgcc agcgtcagtt atatctgcca tgaatttacc aaagagtatc
      121 gtatttcccc tattaactat gttattcaac ggcgtatgac ggaagcgaaa tggtcactca
      181 ctaatactga attatcacag gcagagatct cctggcgtgt gggttatgaa aatgtcgatc
      241 actttgccaa actgtttttg cgccatgtcg gctgttcgcc cagcgattac cgcaggcaat
      301 ttaaaaactg ttttgcggaa caagaaatcc tatctgaatt tcctcaacca gtaagtcttg
      361 ccggataaat ctggcggcgc agtttgttaa gaagctgcgc cgcctgcaga ttatttgttc
      421 tgataatctt tcaggatctg ccgacctgct acgtaaatca taatttcgtc tgtaccgccg
      481 ccgatacgtt cacaacggac atcacgccag aagcgggaga cgcgcgcctc atcggtatag
      541 cccagaccgc ccatgatttg aatcgcatca tcaatgactt ccattgcggt acgtgcgcag
      601 tacagttttg ccagcgccgc gctggtgcgc agtgactgat gctgatcggc ttgccatgcc
      661 actttcaaca ccatgttgcg catgttgtcg atcttaatcg ccatcagcgc cagtttttcc
      721 tggatcatct ggttatgacc aatgggctta ccaaaagcga tacgctggtt ggcatagcgg
      781 gcggcatctt cgaatgcgca ttcggcaaag ccggtgctgc gcgcggcgtt gatcaggcgc
      841 tccatctcaa agttgtacat cacattgagg aaacccattc cttcttcgcc caccatgtcg
      901 ctctcttcaa cttccacgtt gtcgagatag acttcgcagg tgctgagcat atgccagccg
      961 attttatgca gtggattgat tttaatgccg ggtttactgg agtcgaccca ccacagagta
     1021 aaggcctttt tcggatcttt cggttgcgga tcgcgcgcca acaccagcat atatgggtac
     1081 tctttcgcac cggtaataaa ggttttctgt ccgttgatgt aaaccttgcc gtttttacgc
     1141 gtgtaagtgg tagtggcact gttgttgtct gagcctgcgc ctggctccgt caatgccagg
     1201 gcataggcgg gatcacccgt tttcagggtg ctctctgccg ttttacgtag ctgctctgca
     1261 gaaccgaaac ggcgcatact gtgaatacat tgaccgttgg taatcaaaaa tgccggagca
     1321 ccgcattttg acacttccat cagcgccagc atttgggtga cgtaatccgc agggatacca
     1381 ccaaattctt ccggcacgcc aagcatggaa atactgttat ccgccagtgc ccgcataaac
     1441 tcacgcgggt atgtcccgtt ttgatcgcag gtgcggaaat actcttccgg aaagttagtc
     1501 gtaatcagtt cgcgaatact ggtcagcagc agttcttgtt cttcagttaa agaaaaatcc
     1561 atcattatac tcctgttaat gagccgcttc aggcaggaca aaacccatcg ccgcatcgat
     1621 aaataatctt tcgtccatca gtttcagttc tggcgaaatc actggggtga aatccatttt
     1681 gtcgagaata tctttttgta aatcgacgcc aggagcgatt tcgattaaat gcaggccgtc
     1741 ttcttttagc gtgaatacgg cgcgctcagt gatataacga acatccagcc ctcgctcgag
     1801 agcgattttt ccgctgaaag ttatttccgg tagttcccga ataaatttct tcacccgtcc
     1861 ttcctggacg atatttaatt tgccgtcggt aatttctgtt tttaaactgc ccgcagttaa
     1921 tgtgccgcag aacacgattt tcttcgaagt ggcactgata tcaataaatc caccggtgcc
     1981 catgatttta ccattgaatt tatgcacgcc gacgttaccg tgctggtcga cttcagcaaa
     2041 actcaaataa caaacatcca gaccaccacc gtgataaaaa tcaaactggg acgtcatatc
     2101 cagaatggcg cgggtattca cgttcgcgcc aaaggcgatc ccctgtgaag taataccgcc
     2161 aatcggacct gtttctaccg tcagaataaa gtcatcagca caaccttctt cccgggcgac
     2221 caggccaatg ccgtcagcaa taccgacgcc gacattcccc actgcgcctt tgcgcatttc
     2281 gaataatgca cgccgtgcaa ctaatttacg ttggtttagg ggcaggctaa gtttggtgct
     2341 gtcatcaaga gtgaagtcac cagaaataaa gcggttaacc ggcgcaccgc catacagttg
     2401 ggtttgatcc ggatcgacca ccacaatatc caccagataa cccggaatac ggacagattt
     2461 aggatgcagc gtggctttct taaccatttt ctgcacctgc atcatcacaa taccgccgtt
     2521 attgtgcacc gcctgggcaa taaccaatgc gtcgagatac atcacctcat cttcaaaagt
     2581 ggcgtagcct tcgctgtcgc aggtggtagc gcgaatgaag gcgatatctg gcgctatcgc
     2641 tttgtaatag agatattctt tgttatcaaa ctcgaccagt ttaatcaggt cttctttagt
     2701 gacttcattc agtttgccgc cttgctggcg tggatcgaca aatgttccga tgccaatatc
     2761 actaataata ccaggctggt gggctgcggc ggcgcgtaag gtttgtgtaa gtacaccttg
     2821 tggatagtta taagcaataa ttttattttg ttctgcgaga tcagaaatac gcggcgattg
     2881 tccccagtga ccgcataatg cccatttcac cagaccttct tgcgccagcg gactaatacc
     2941 acggtcggcg cgatcgccaa gccctgttgg actaataatc gataaattac gtggtgtttg
     3001 agtctgttta tatttatcag caagagcagt aattaacgtg gtggcttcca gaataccgcc
     3061 gccagcgcct aacacacaaa gtgttgcttc gtcgggaata taattcaccg cttcctgtgc
     3121 cgacaggacc ggcactcgtc cattaatacg aggtggtttt acaggtttca ttgttaattc
     3181 ctgattcagg tgtgattttc cctacgccag atggagaatt agcgcacaga gactcacgaa
     3241 atatttttat taaatacggt taaaaatact ttttttagtt cggcgggaga gggtattccc
     3301 gccgaaaata ttattgctta tgcctgatgt aaaatagtta ataccgtacg caaatcattt
     3361 accgagattt gccctggcgc agaggcttgt tttaccgcac caaaagttgc cgccgaccca
     3421 aatacttcac cagccagacg agaaattacg ccagtttttg ccatcgacat cgtgatgatt
     3481 ggacgatcgg catactgctc ctgcatctcc agggtcgcgg caagcaacgt cagcacatcg
     3541 ctggtacttt gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga ctgcattttg
     3601 cgcagacggg caatgatttc ttcggcttcc ggcgttttat ggaagtcatg gttggacatg
     3661 acaactttca catcatgcgc gtgggcgtag gcgacggttt ctttaacctg atcatcgccg
     3721 gtaaataact ccagatcgat catatcaacc aggccgctgt cgatggctgc acgattgagt
     3781 gcaatataag cctcggtgga aatcgcctgc tcgccgcctt ctttggcact gcggaaggta
     3841 aacagcagcg gtttttctgg catggtttca cggagaattt ttgctgccgc catgacagac
     3901 tccacattgg agaggtcggc atagtggtcc acacgccatt ccagaatatc aaagtccgct
     3961 tcacgatagg cgagagcttc ggatttcacg ctggcgatat ctttcgccat cagcgagacg
     4021 atgattttag gtgcgcccgt accaatgacg agatctttta cagttacggt tttcattttt
     4081 taccctttct gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg tttaacatat
     4141 tccagaggga aatctttgcc agtccataac gtgaactgtt cagccccttg ccacaacaac
     4201 atgccgtatc catcaatcgt tttgcaaccc gcttgttgcg cctgctgcaa taacttcgtc
     4261 atatgcgggt tatacacgca ttcagtgacc agaagtcccg gatgtaacag actgatatca
     4321 ttaaccaatg attcattctc aaggggtttc atacccactt tggtgccatt ggttaaaatg
     4381 tcggcggaag ccagggcttc tgcaaaggct tgctgatcgg cgagatcggt gactgtcacg
     4441 acgcaatcgg tgttttcgtt aacccgctgc gcgaaggcga gggctttatc gaagaactca
     4501 tcccgacggt taaagagttt aatttctttt aaaccttcaa ttgccccctg cgcgccaatt
     4561 gccgttgagg cacctccggc ccctaacagc accatcgttt tgcctttgat atcaaaaccg
     4621 ctctctttaa tggcgcgaat atggcccgtg ccgtcggtgt tatagccacg cagatagcca
     4681 tcatcattta cgatggtgtt gatggcaccc accagtttgg ctgctggcgt taattcatca
     4741 acatattcac acgccagttg tttgttcggc attgatacac cagttccgcg cattttgagg
     4801 gcttttaatc cttcaattgc tccgggaaaa ctatcgttat ccacttcaaa ggccatatag
     4861 gtaaatggca atcccgcttt ttctaaggct ttattctgca tttcgggcga taaactgtgg
     4921 cggatgggat aggccatcaa cccaatcaat tcgtattttg cggtaacatc cataattaac
     4981 tcctttaacc tctatgctta attgtgttta accgggtgct aaatttatgc tcgccaaacc
     5041 gcacatcatt ttcaggaata atgaaaacgc ggtaatagcg gataaaaaca ataattgcgg
     5101 taatcagggc cagcagcgcg aaagtaaaat cgagaacaat gatatattgc aggccgatgt
     5161 tcgacagata accggtaatc agcggaataa caaagttagc cagtccaccc atcatcatat
     5221 atatactggt gactttggct ttgcttttgg ggaaaaactc tgacatgacc gaaacgccga
     5281 gctgtaaaat gccgccagct gctgagaaac cgataacaaa ggctccggca ttacacacca
     5341 atggagaagg gtacaggtag ataatggcag ctgttattgt tgccagtgca gagttaaata
     5401 cattagccca gatgggccgg accatttttt tcagcagtgc ggcaaaaata aagacacaga
     5461 ccagcgaacc catactgtaa taagagatgg tttttaatgc ctcagcttct gacataccag
     5521 caaaagccat cgcatatttg ggcatccaca ccacaatcac ataaaaggtc gagaatgcgg
     5581 ctacaccgaa cagtactgat gaaacacctt ccagccagac taacggtttg ctgttcattt
     5641 gcggtaattc attagctacg ctggcgtcca ccaactggct ggggaatttg cttttcaaca
     5701 gcatcagcgt gatcagtaca aacagaatac ccggaataat cagcccatag ccgtaccaga
     5761 tgttattgag caacatatag ctcaccagca ttgggtagaa catttgcccg aatgacacca
     5821 tcgctttaac cagtatgacc gccgaaccag aggctttcgg aaagcattcc ataagcgcgg
     5881 ggtagccacc cgtatccagc gctgagttag cgatacctac gcacactgcc agacagtagg
     5941 cgagagttaa attcgggcaa gcaggaatac caaagaagaa tagcagatac attattactg
     6001 ccattaatat caccgcccga cgaccaaact tatcggagat cactccgaag aataaaatac
     6061 tgatcaatcg ccccaatccg ataccggaaa ttaaataggc aatgcccgcg ttgtcagtgg
     6121 aaaacttttc tgccaaagat gtcatgtttt gggcaagcgt aataacacta atgccgtgca
     6181 ggaaatagct gaagtaaata caaagaacag ccaggataaa tggcgtgctg aaagccttat
     6241 tttgagacat tttcacagct ccttgctgaa agataatttc gttaccgtac gtattgttct
     6301 ccgacggttt acataattac tatactgaag atgattttcc ctgggtacat ggcataaagt
     6361 ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt tccttagtta
     6421 gcgcagaaag agttatttat ccgtaagatc ttgcataacc tgaaaagaat attgcattac
     6481 ccacccggag cgatattttc ctttagcgtg tgatgccgcg tttctttgcg actgcgtaag
     6541 ccaataaaca gtgccagtat aaagccgata gcagcgatgg cggtatcaaa ccacataata
     6601 tcggcaatac ttctttgcga aagatgagcc gtaatcaacg gaatggtaaa tgttgcaatg
     6661 ctgcccgcac tgtaatagat ccccgttgct tttcctttag cgtagggaaa acgttccgcc
     6721 attaacgtca ggccaatttg tacaacaccg cctgcggagg taaaaccgat gacgaaagca
     6781 aatattatca ccacataaaa tgtgggatgc aggcagacgg tgagcagggc gataaatgag
     6841 ataaaggtgt acagcatcag taatgttgtt gggcgaacgg tattacgaat gagtggagca
     6901 gtaataaata cacacagcag cgaacccacg gtgtagatac tgagtaattt gatcgacatg
     6961 gtgtatgaca tgccagcgac aaattgtccg tattgtgcca gccactggct aaccagataa
     7021 aacgttgcca ttgagatata gccatatagg gaataactgg ctaaatcgat aattgaacag
     7081 cgatgttccg tagagctggt ggttttcttt atgacaggta agtgacgacc cggatggggt
     7141 gggaacgtac aacggtataa aaacagagcg ttaataaaca taatgcctgc ggcaatcata
     7201 aaggaccaac cgaaccacag ttcagcccac actaacaggc tgataattag cggtaataaa
     7261 aactgtccgc tggaaacaaa tgctttaatt aaaatattgg ctgtacccgg tgagcgtgga
     7321 aaagcttcca tcaaactggg ataggtgcct gcatcgagaa aactgtttgc cattcccgcc
     7381 agaaagccaa aaacataagc gataatgatg ttattggtgt gcaggatgcc aaaaaagaag
     7441 gccatatagc agcacatccc gagcatgata aaagggcggc gaccaaagcg atcggataat
     7501 aatcctgcaa aaagcaagac acttaatcga ccaatgccca gtgatgagat aactatcgag
     7561 acgcccgcgg cattagtctg ccaaagcgtc tccagcgagg ccatattcag gctcatcaaa
     7621 atgacgccca taccatgcac caggtaatta aaatacaacc caagtgcggt agggtaatag
     7681 ggatttttca taggttctac ttagcaaagg tgctttaaaa agtgtagcga tgttgtgtca
     7741 gtatccgttc attattgttg taacaacgac aattcctttc tgtggagaaa ttaacaaagc
     7801 cgctccagat cgtgtgcata taattcagcg gctaccgatt gatagatctt ccagtcaata
     7861 aaattgccat cagggtagac ttgttgaaac gcggttaagt cggggaaaaa acgcatggtg
     7921 ttgttgccga tgcggttatt tattttctct atgatggcat gaagatgttt ttttcgttgc
     7981 tgacgcatag ccaacagttg ctcgactaca caatcaggaa tggcgcattt gccattttcc
     8041 cactgacgcc agctttcact gtcaccggtc tgggcaatcc aggtcgcgca ttcatcaata
     8101 gtcatggcaa aaatatggcg aagtgcctgg agttcataag cgttcatcgt gtaatggctg
     8161 catgaaataa acaataaaat ataaataaca ttatcagcct gctgacggct tgaagagagt
     8221 cacagtatct tgtgcaacat tattggaaag agtgaggggc gaggtagcga agcagaaaaa
     8281 tgttaaaaaa aagcccatcg tgggagatgg gcaaagacta cacacagcaa ttcgttgttt
     8341 cactcagggg atttccatgc ttataaatca atatgttgat ttataaccgt gagctaatag
     8401 taggcatacg gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa actgtaaaga
     8461 atttgcgaca ggtactacca gctgaatagc ggtaagcagg aaaatatggg gaagagcaag
     8521 caaatatcaa ttagcagggg ttaacaatca ggcggcagag ccgcctgatg agaaattact
     8581 tattcgattt ctcgatttcg ccaagttctt cgagaatttc ttccggttgg tccgtcggcg
     8641 gtgggacttc ttccacccac gcggcaaaca gacgccagga aacggctaac agaaccggac
     8701 caataaacag accgatcatc ccgaaagcaa tcaaaccacc aataacgcca gagaggatca
     8761 ggatcagcgg taaatcggca cccatgcgaa ttaacattgg gcggatgacg ttatcgagcg
     8821 tgccaaccac cccgctccaa actaacaata ccgttcccca ggtggtatcg ccagtccagt
     8881 agagccagat aatcgccgga atcagtaccg gcaacggtcc aagctggaca aggcaggaga
     8941 ggatcattaa caccgttagc aaagttgcat aaggtacgcc ggatacggcg aggccgatac
     9001 cgccaagcac tgcctgtact aacgccgtca ccaccacacc cagcgccacc gcgcggatag
     9061 cctgtgccgc cagcagcacg gcggcatctc cgcgaacgcc tgccagacgg gttgcaaaat
     9121 ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag cagggcgctg aagagaagca
     9181 tcagcgcaca atgcaccata aagcgcccga tatgcgccgc ctgcccaacg aaccaggtgg
     9241 tggtggtgcc aatataagga cggactttcg ccatgatcgc cgtccccccc atatccagca
     9301 aattgtgcca gcctgcatat agcttcgcgc caatcaccgg aatggtatta agccacgcca
     9361 gatcgggtaa cgtcatgtca ccactggaaa tggctttaat tagcgggccg ctgccgtcga
     9421 cgatactgtt aaccagcaaa gcaataggga tgataaacac catcactaat aacagcgtca
     9481 tcaccagaac ggcgagagag cggcgaccaa acatgatctt ttgcaaacgt aacaataccg
     9541 gccaggtggc gataaccact gtaccggccc atgcaaagcc gagaataaag ggttgtacaa
     9601 tccacagaca tgccacaatc atgatggcta aaaacagcac cgaaagcaga atttgtgcga
     9661 catccctggg ctgacgaaca tttaccataa ctacttttca ccttttttgt gcgtcaaatc
     9721 gtcgacgcga cgtgaacacg cgaaactcac ctgcataatg atgagcaatt tcagcggttt
     9781 ttaacaggtc gattctgccc ttaattctga tgggcgtgtt agaaaaaaat gtggtacaac
     9841 aactcaggca acacgcaaac gattaatctc tcatcacaga ccgcaggaaa gggtcaatat
     9901 aatgattcca cagatttccc aggcacccgg cgtcgttcaa ctggtgctta attttttgca
     9961 agagctggag caacagggtt ttaccggcga tacggcgaca agttatgccg atcgtctgac
    10021 aatgtcgact gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc
    10081 aaccgcagat gtagcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgttgat
    10141 ctttaccccc cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat
    10201 tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt
    10261 gcgcgttgag gccggggtga ttaaagacca actcaatcag tacctgaaac cctttggcta
    10321 cttttttgcg ccagaacttt cgaccagcaa ccgggcaacg ctcggcggga tgatcaatac
    10381 cgatgcatcc ggtcagggat cgctggtcta tggcaaaacg tcagatcacg tacttggcgt
    10441 acgcgcggtg ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgaactggc
    10501 ggaaacgctg ggtaaatcca ataccataat cgggcgaatt tataacacgg tttatcaacg
    10561 ttgccgtcag caacgccagt taatcatcga caacttcccc aagcttaacc gctttcttac
    10621 cggttacgat ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat
    10681 tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcgactgg atattacgcg
    10741 cttgcctaaa gtacgccgtc tggtgaacgt caaatatgac tcttttgact ccgcgctgcg
    10801 taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt
    10861 gctcaacctt gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt
    10921 gcctgataaa gagatgctcg ggctgaacat tgtggaattt gccggtgatg atgaggcact
    10981 gattgacgag cgagttaatg cactctgtgt gcggcttgat gagctgattg ccagccaaca
    11041 ggcaggagtg atcggctggc aggtgtgccg cgagctggcg ggtgttgaac gtatctatgc
    11101 gatgcgcaaa aaagccgttg gtctgcttgg caatgccaaa ggtgctgcta agccaattcc
    11161 gtttgctgag gatacctgcg taccgccgga acacctggcg gattatattg ctgaatttcg
    11221 cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt
    11281 cttgcacgtc cgtccagcac tggatatgtg cgatccgcaa caggagattt tgatgaagca
    11341 aatctctgat gatgtagtgg cgcttaccgc gaaatacggt ggtttgttgt ggggcgaaca
    11401 cggcaaaggt tttcgcgccg aatacagccc ggcgtttttt ggtgaggaac tttttgcaga
    11461 actgcgtaaa gtgaaagcgg cgtttgatcc gcataaccga ctcaaccccg ggaagatttg
    11521 cccgccagaa ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt
    11581 cgatcgacag atccccattg cggtacgcca gcagtggcgt ggtgcaatgg agtgtaacgg
    11641 caacggttta tgcttcaact ttgatgctcg tagtccgatg tgtccgtcga tgaagatcac
    11701 ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgctt
    11761 gttggcggat cgcggcgttg acccactcaa actggaacaa gaactgcctg aatccggcgt
    11821 cagtttgcgg acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata
    11881 tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcttgttc
    11941 gactcagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgtttcc tgcaactcta
    12001 tcacacccgt tatttacgcc cgctgcgcga ccacctcgtc gctacggttg agagttacgc
    12061 accgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg
    12121 caaactctcg gaaaaacata tcggcatgat tgatttgccg ctgctgtcgg tcccctcgct
    12181 acaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaagctct
    12241 caatgcagag cagaaagcgc gcacggtgct ggtggtgcag gaccccttta ccagctatta
    12301 cgatgctcaa gtggtggcgg attttgtccg tctggtcgaa aaattaggtt tccagcccgt
    12361 gttactgcca ttttcgccaa atggaaaagc cctgcatatc aaaggttttc ttaatcgttt
    12421 tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc tgggtatgcc
    12481 aatggtaggc gtcgacccgg cgctggtact ttgttatcgc gatgaatata aactggctct
    12541 gggcgaggag cgtggcgagt ttaacgtctt actggcgaat gaatggctgg caagcgcact
    12601 tgagtcacag ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac
    12661 cgaagttacc gccttgccgg gcgcgccagc acaatgggcc gcgatatttg cccgttttgg
    12721 cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga
    12781 agcgaaaaac cataaaaatt cgctcgggat ctatgagtta tcctggcatc aggcgatgca
    12841 gcgactgccg cgtaatcgct gtctggcgac cggatattcc tgccgtagcc aggtaaaacg
    12901 ggttgaaggc acaggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata
    12961 tggaaacgaa aaatcaccct ggaagcactg aatgctatgg gggaaggaaa catggtgggg
    13021 ttgctggata ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta
    13081 gactcgcgga caaagcagcc tttcgggttg ctgcatggag gtgcatccgt ggtactggcc
    13141 gaaagtatcg gttccgttgc cggttattta tgtaccgagg gtgagcaaaa agtggttggt
    13201 ctggaaatca atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg cggcgtatgc
    13261 aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa
    13321 gggcgtttgt gctgttcgtc acgattgacg accgctattt tgtgacgttg tcaccagaaa
    13381 agtgtgacgc atattgcttt tggttattat ctgcacagta aacggtggta tactgtgcag
    13441 gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca actggccatt
    13501 gaatttttac gccgtgacca aagcaatctt tcacctgccc aatatctcaa gcgtctgaaa
    13561 caattagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa
    13621 atctacttcg cctggcgtct gggcgtgcat tgatcctgat ttaatgtcgc gagttcgcca
    13681 aatcggcctg gattaatctt cgtccaggtt cgagggcctt tttaatacag cctatctacc
    13741 cgcctgataa cgttcaaaaa taatacaaat cgcagtttgt gttaaaacag tgggttagct
    13801 ttatgagatt atccttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag
    13861 cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt
    13921 ctgaacgctg ctctttttta cctatccctt aaatagtcat tttcaatgca actttttctt
    13981 tttcatcaca gccttaatgc tccttagata ataatcatta tctaacaatg agatacctaa
    14041 ttcttagcgt gcctgttaac ccacaaatca gggtctatgc ttattaaaag caggtaaaaa
    14101 gcatgtttta cgctaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa
    14161 catgctgtta tacgctgaaa gcgatgaagt gaggtaaatc gatggacatg cattcaggaa
    14221 cctttaatcc acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac
    14281 acatccgcga actggtggca aagcagccgg gtatgatcgg cgtgcgctta ggcgtgaagc
    14341 aaacaggctg cgcgggcttt ggctatgtgc tcgatagtgt tagcgagccg gacaaagacg
    14401 atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc gctgcaagcg atgcctttta
    14461 ttgatggcac ggaagtcgat ttcgttcgcg aaggacttaa tcagatattc aaatttcaca
    14521 accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt tggggtatag gcggtactat
    14581 gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta
    14641 taaagaagga ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga
    14701 ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct
    14761 caacgcctat cacgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga
    14821 caagctgaat tatcaggatt acagctacta ctcggcacca tcgtgcggta attgtgacga
    14881 caactgcgcg tctgaacccg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa
    14941 agaggtggag gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc
    15001 agtggatgcc attttcgact ctgtttcggt tgccactact tatcgcaaaa aactggcgga
    15061 gcagggaatt attttttgtt cctttggcga ggcgatccac gatcacccgg aactggtgcg
    15121 taaatatctc ggcaccgtgg tgccggggaa tgacaacttc tttgccgcac ttaatgcggc
    15181 ggtagcctct gacggtacgt ttatttatgt gcctaaaggc gtgcgttgcc cgatggaact
    15241 ttccacctat tttcgcatta acgcggaaaa aaccgggcag tttgagcgca ccattctggt
    15301 ggccgacgaa gacagctacg ttagctacat tgaaggctgt tccgctccgg tgcgtgacag
    15361 ctatcagtta cacgcggcgg tggtggaagt catcatccat aaaaacgccg aggtgaaata
    15421 ttccacggta caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt
    15481 caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac
    15541 cgggtcagcg attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg
    15601 tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat
    15661 gatccacatc ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca
    15721 tagtcagaac agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa
    15781 tttcactcag tgcgactcaa tgttgattgg cgctaattgt ggggcgcata ccttcccgta
    15841 tgtcgaatgt cgcaataaca gcgctcagct ggagcacgag gcaacgacat cacgtattgg
    15901 tgaagatcaa ctgttttact gcctgcaacg cgggatcagc gaagaagatg ccatctcgat
    15961 gattgttaac ggtttttgca aagacgtgtt ctcggaactg ccgctggaat ttgccgttga
    16021 agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt
    16081 aagtattaaa ggtttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct
    16141 cgacgttcgt cccggcgaag ttcacgccat tatggggccc aatggttcgg gcaaaagtac
    16201 cttatcggca acgcttgccg ggcgagaaga ttatgaagtg acgagcggca cggttgagtt
    16261 taaaggcaaa gatttgcttg cgctgtcgcc ggaagatcgt gcaggtgaag gcatctttat
    16321 ggccttccag tatccggtgg aaattcccgg cgtcagtaac cagtttttcc tgcaaacggc
    16381 acttaatgcg gtgcgcagct atcgcggcca ggaaacgctc gaccgctttg attttcagga
    16441 tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt
    16501 aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct
    16561 ggaaccggag ttatgcattc ttgatgagtc agactccggg ctggatattg acgcattaaa
    16621 agtggttgcc gatggcgtga actcgctgcg tgacggcaag cgttcattca tcattgttac
    16681 gcactaccaa cgcattctcg actacatcaa gcctgattac gtccatgtgc tgtatcaggg
    16741 acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg
    16801 ctggcttacc gaacagcagt aacgcactgc aacagtggaa tcatttgttt gaagctgaag
    16861 gggcgaaacg ctccccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc
    16921 cgacacgtaa acatgaaaac tggaaatata cgccgctgga cgggctgacc aatagccagt
    16981 ttgtcagcat tgcgggggag atagccccac agcagcgtga tgccttagcg ttaacgttag
    17041 acgccgtgcg gctggtgttt gtcgatggac gttacgtgcc cgcactgagc gatgcaactg
    17101 aaggcagcgg atatgaagtg agcattaacg acgaccgtca agggttaccc gacgctattc
    17161 aggcggaagt gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg
    17221 tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg
    17281 caggtgaaga ggtgaatact gcccattacc ggcatcatct ggatctggcg gaaggtgcag
    17341 aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc tcgtcacttc accggcgcac
    17401 ggttcactat caacgtcgca gcgaacgccc atttgcagca tatcaagctg gcgtttgaaa
    17461 acccgctcag tcaccacttt gctcataacg atttgttgct ggctgatgat gccaccgcat
    17521 ttagccacag tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca
    17581 atggcgaaaa cagcacggtg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt
    17641 gtgatactcg tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca
    17701 aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc
    17761 acgccatcaa aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg
    17821 aagtggatac taaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg
    17881 cgacggtggg gcgtattgat gacgaacaga tgttttatct gcgctcgcgc gggatcaatc
    17941 agcaagatgc ccagcagatg atcatctacg cctttgctgc cgaactgacg gaagcactgt
    18001 gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg tcaacgactg ccaggaggtg
    18061 caagatgact ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt ctcgtgaggt
    18121 aaacggtttg ccgctggctt atctcgacag cgccgccagt gcgcagaaac cgagccaggt
    18181 gattgatgcc gaggctgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca
    18241 taccttaagc gcccaggcga ccgagaaaat ggagaacgtg cgcaagcgtg cttcgctgtt
    18301 tattaatgcc cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa
    18361 tctggtcgcc aatagctggg gcaacagcaa tgtgcgagcg ggcgataaca tcatcatcag
    18421 tcagatggag caccacgcta acattgtccc ctggcagatg ctttgtgcac gcgttggcgc
    18481 agagctgcgt gtgatcccgc tcaaccccga cggtactctg caactggaga cgctgcctac
    18541 gctgtttgat gagaaaactc gtctgctggc aattactcat gtctccaacg tgcttgggac
    18601 agaaaatccg ctggcggaga tgatcacact tgcgcatcaa catggcgcaa aagtgctggt
    18661 ggatggcgct caggcggtga tgcatcatcc ggtggatgtt caggcgctgg attgcgattt
    18721 ttacgtgttc tccgggcata aactgtatgg ccctaccgga attggcattc tttatgtcaa
    18781 agaagccttg ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt
    18841 cagcctgagt gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc
    18901 caataccgga ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct
    18961 taataacata gccgagtatg aacagaatct gatgcattac gcgttatcac agctggaatc
    19021 tgtaccggat ctcactctct atggtcccca gaacagactt ggcgttattg cttttaatct
    19081 tggtaaacac cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg
    19141 taccggacat cactgcgcaa tgccattaat ggcctattac aacgtccctg cgatgtgtcg
    19201 ggcatcgctg gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca
    19261 acgtattcac cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga
    19321 aaaattgctg cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat
    19381 tgagttgggt cagcgtctgc cagaattacg tgacgaagac aaaagcccgc aaaatagcat
    19441 ccagggctgc cagagtcagg tgtggattgt gatgcgtcaa aatgcgcagg gaattattga
    19501 attacagggc gacagcgatg cggccattgt aaaagggctt attgcggtcg tctttattct
    19561 ttacgatcaa atgacgccgc aggatattgt caatttcgat gtgcgcccgt ggtttgaaaa
    19621 aatggcgctc acccaacatc tcaccccatc acgctcacaa ggtctggaag cgatgattcg
    19681 cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt
    19741 ggctcacatt gccgggtggt caatgtttac actggcctgc acaggagtgt tttggtttca
    19801 ggtgaacata agcgccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta
    19861 caatgaaacg cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg
    19921 ctgcctgggc ggttgattat ccgctaccac caaccggcag tcgactggtt gggcaaaatc
    19981 aaacgtatac ggtgcaagaa ggggataaaa accttcaggc catcgcccga cgttttgata
    20041 ctgcggcaat gttgatcctt gaagccaata acactatcgc cccggtgcca aaacccggta
    20101 cgacgataac tattccttca caactattat tacctgatgc gccgcgtcag gggattattg
    20161 taaaccttgc agagctgcgc ctttataatt atccgccggg agaaaatatc gtgcaggttt
    20221 atccgatagg tattggtttg caggggctgg aaacgccggt gatggaaacg cgtgtggggc
    20281 agaaaatccc taacccaacc tggacgccta cggcgggcat tcgtcaacgt tcgctggaac
    20341 gtggcattaa attaccacca gtcgttcctg ccgggcccaa taacccacta ggacgttacg
    20401 cactgcgcct cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca
    20461 gcgtcggttt gcgcgtcagt tcgggctgta ttcgcatgaa tgcaccggat attaaagctt
    20521 tattctccag cgtgcggacg ggaacgccgg tgaaagtgat caacgagccg gtgaaatatt
    20581 ccgtggaacc taacgggatg cgttatgttg aagtacatcg accactatcg gcagaagaac
    20641 agcagaacgt tcagacaatg ccgtacacgc tgccagcagg ctttacgcaa tttaaagaca
    20701 ataaggctgt agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg
    20761 ttgcggtgag cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc
    20821 agaatgggga accagagcaa gggaatatgt tacgcgcgac gcagtagcgg taaatagcag
    20881 gcaaaaaaat ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt
    20941 atttagtagc catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct
    21001 gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag
    21061 tctgaacgtc agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag
    21121 tagaacccag gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct
    21181 agattgagtt aatctccatg tagcgttaca agtattacac aaagtttttt atgttgagaa
    21241 tatttttttg atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct
    21301 gggttttaga taaaaaaatt gagagaaa
//
LOCUS       DAZKHK010000091        21169 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKHK010000091 DAZKHK010000000
VERSION     DAZKHK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.091"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(6..827)
                     /gene="dapB"
                     /locus_tag="ACHOS5_003438"
     CDS             complement(6..827)
                     /gene="dapB"
                     /locus_tag="ACHOS5_003438"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9778201.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(988..1353)
                     /locus_tag="ACHOS5_003439"
     CDS             complement(988..1353)
                     /locus_tag="ACHOS5_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9778202.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1376..2290)
                     /gene="rihC"
                     /locus_tag="ACHOS5_003440"
     CDS             complement(1376..2290)
                     /gene="rihC"
                     /locus_tag="ACHOS5_003440"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP9778203.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFTPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLEVP
                     AFLAIRDALLRAPEPVTLVAIGPLTNIALLLSQCPECKPYICRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2356..3306)
                     /gene="ispH"
                     /locus_tag="ACHOS5_003441"
                     /gene_synonym="lytB"
     CDS             complement(2356..3306)
                     /gene="ispH"
                     /locus_tag="ACHOS5_003441"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP9778204.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDATDIQEEWVKEAKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3308..3757)
                     /gene="fkpB"
                     /locus_tag="ACHOS5_003442"
     CDS             complement(3308..3757)
                     /gene="fkpB"
                     /locus_tag="ACHOS5_003442"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9778205.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3865..4359)
                     /gene="lspA"
                     /locus_tag="ACHOS5_003443"
     CDS             complement(3865..4359)
                     /gene="lspA"
                     /locus_tag="ACHOS5_003443"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9778206.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4359..7175)
                     /gene="ileS"
                     /locus_tag="ACHOS5_003444"
     CDS             complement(4359..7175)
                     /gene="ileS"
                     /locus_tag="ACHOS5_003444"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9778207.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDTFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7218..8159)
                     /gene="ribF"
                     /locus_tag="ACHOS5_003445"
     CDS             complement(7218..8159)
                     /gene="ribF"
                     /locus_tag="ACHOS5_003445"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP9778208.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8167..8385)
                     /locus_tag="ACHOS5_003446"
     CDS             complement(8167..8385)
                     /locus_tag="ACHOS5_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP9778209.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8488..8751
                     /gene="rpsT"
                     /locus_tag="ACHOS5_003447"
     CDS             8488..8751
                     /gene="rpsT"
                     /locus_tag="ACHOS5_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9778210.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8847..9752)
                     /gene="nhaR"
                     /locus_tag="ACHOS5_003448"
     CDS             complement(8847..9752)
                     /gene="nhaR"
                     /locus_tag="ACHOS5_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP9778211.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9812..10978)
                     /gene="nhaA"
                     /locus_tag="ACHOS5_003449"
     CDS             complement(9812..10978)
                     /gene="nhaA"
                     /locus_tag="ACHOS5_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP9778212.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            complement(11213..12472)
                     /locus_tag="ACHOS5_003450"
     CDS             complement(11213..12472)
                     /locus_tag="ACHOS5_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFP9778213.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            complement(12601..14094)
                     /locus_tag="ACHOS5_003451"
     CDS             complement(12601..14094)
                     /locus_tag="ACHOS5_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9778214.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAMGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKKPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            complement(14115..14876)
                     /locus_tag="ACHOS5_003452"
     CDS             complement(14115..14876)
                     /locus_tag="ACHOS5_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP9778215.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            15491..15643
                     /gene="mokC"
                     /locus_tag="ACHOS5_003453"
     CDS             15491..15643
                     /gene="mokC"
                     /locus_tag="ACHOS5_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP9778216.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(15748..16878)
                     /gene="dnaJ"
                     /locus_tag="ACHOS5_003454"
     CDS             complement(15748..16878)
                     /gene="dnaJ"
                     /locus_tag="ACHOS5_003454"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9778217.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(16967..18883)
                     /gene="dnaK"
                     /locus_tag="ACHOS5_003455"
     CDS             complement(16967..18883)
                     /gene="dnaK"
                     /locus_tag="ACHOS5_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9778218.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            19255..19659
                     /locus_tag="ACHOS5_003456"
     CDS             19255..19659
                     /locus_tag="ACHOS5_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP9778219.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            19685..20398
                     /gene="msyB"
                     /locus_tag="ACHOS5_003457"
     CDS             19685..20398
                     /gene="msyB"
                     /locus_tag="ACHOS5_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9778220.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            20547..21113
                     /gene="satP"
                     /locus_tag="ACHOS5_003458"
     CDS             20547..21113
                     /gene="satP"
                     /locus_tag="ACHOS5_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP9778221.1"
                     /translation="MGNTKLANPAPLGLIGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
BASE COUNT         5360 a         5579 c         4936 g         5294 t
ORIGIN      
        1 tgtggttaca gattattgag atcaagcaca tctcgcatat caaaaagacc actttcctta
       61 ccactcaacc acaaagccga tcttaccgca ccgttagcaa atgtcatgcg gctggatgcc
      121 ttatgggtga tctccagacg ctcgccaata tcggcaaaca tcgcggtatg ttcgccaacg
      181 atgtcacctg cacgcacggt ggcaaaacca atggtgccag gcacacgttc accggtatgg
      241 ccttcacgac tgtagaccgc gcaatctttc agatctttat caagagcgtg ggcgatcgcc
      301 tctcccattg ccagtgcggt gcctgacggc gcatcaactt tatgtctatg atgtgcttca
      361 ataatttcga tatcggtgta gtcccccatc actttggctg ctttctccag cagtttaagc
      421 atgacgttaa cgccaacgct aaaattggcg gcaaagacaa tcgcaatatc ggcggcggcg
      481 tcacgaatcg cctgtttacc ggcttcgtca aaccccgtag tgccgatcac catccctttg
      541 ccatgctggc gacaaaaagc gagatggttc agcgtacctt ccggacgggt aaaatcgata
      601 aacacatcaa aatcatcttt tatcgcatca agactacttt gcacagtaac gcctgttttc
      661 ccggctccgg ccagttcgcc cgcatcgctg cccagtaaag aagatccttc gcgctccagc
      721 gccgcgccca gctgcacgcc ttctaatgcc agcgccgcct gaatcaactg gcggcccata
      781 cggcccccgg ctcccgcgat ggcaacgcgg atgtttgcat catgcatagc tattctcttt
      841 tgttaatttg catagaccgt tttcagagta accagcgcat cgctacgccg ccagccaaaa
      901 acgctgataa ttagaaatta atgataaaca gggagggata tcagtgaaag gcatgacgca
      961 gaaagtgatg cgtcataccc tctgtcatta tgcctggtct tgtgactttg acggcaggca
     1021 gcacagtata agcagaatta cgcccgtaac agggatcaac acgacaatgg taacccacca
     1081 ccctgaatat ccgatatcgt gcatccttct gacggctaac gctatcgccg gtacggcaaa
     1141 aatcaacgtt aaaataaatg acgcgggcag cgcaaaaaaa atgaaaacac catagtcatt
     1201 gttaattgtt accgctaacc accaacataa aagcggaagc agacagataa cgccactacc
     1261 aatcataaac gaccagaact cgaagcgatt ggaaacacct tcataattaa agatgttttt
     1321 ccacccttcc agatacgctt ctccatacgt catagaacac ttccttgttt tattattaga
     1381 gcgccagggc cagcacctca gccacccatt gctggaagcc tttcacatcc agatccagcg
     1441 ccacctgtac attggctggt ttgcccaggc aaccgtcgat atcaacaacc gtcgtgcctg
     1501 aggtaaattc accctgagtt tctaccgcga caaaacacgg tttgagagtg aacaattccg
     1561 ggcgcaccag ccaggcgata gcgcaaagat cgtgcatacg tagtccactt tgcatactgc
     1621 cgctacggta gtggctaaac agggcgtgta gcatttttcc ggtgcgattc aattccggca
     1681 aagtagcgag atagtcaggg gttaatattg cctgattggt gacatccaga ccgcacatga
     1741 cgatttcgat gccgctacgg aagacacagg ctgccgcttc tggatcggca gcgatattaa
     1801 actcggcgtt tggtgtgcag ttgccgcgac cagcagaacc gcccatgatc accagacggc
     1861 aaatatacgg cttacattcc gggcattgtg aaagtaacag cgcaatattg gttaacgggc
     1921 cgatggctac cagagtaaca ggctctggcg cacgcagcag ggcatcccga atcgccagaa
     1981 acgccggtac ctcgagcggc tttcggttgt gctcaacaaa gtcgtagcca gccattcccg
     2041 attcgccgtg cacagatgcc gcatcgcgcg gcgcacgaac cagtggtacg gcagcgccct
     2101 gggcaagcgg aatatccaca ttccagaaat gcagcagttg cagggcgttg cgggtcgttt
     2161 tctctaccga aacattaccc gcgacagtgg tcatcagttg cagatcgagt tcgggggtaa
     2221 aaatcgcggc ggcaatggca acggcatcgt caatgccggg gtctgtatcg aggaagatag
     2281 gtaaacgcat gttttctcca taaaaaatgc cggtaacaag accggcattt tcgcataact
     2341 taggctgcta atgacttaat cgacttcacg aatatcgaca cgcagctctt tcggcacttc
     2401 gaaaacaatg ttttcttcac gaccttccag tggaatggct tcgccaccgc caagctgctg
     2461 taaacgtgcg actacgttct gtaccagaat atccggagct gatgcgccag cagttacgcc
     2521 gacgcatttc gcctctttca cccactcttc ctggatatct gtcgcatcgt caatcaaaaa
     2581 cgcgcgcttg cccatacgct gggccagttc cgccagacgg ttggagttgg aggagttttt
     2641 cgaaccaacc accagtacta cttccgcctg ctctgccagg gcgcgtaccg cttcctgacg
     2701 gttagtcgtg gcgtagcaga tgtcatcttt gcgcggaccg acaattttcg ggaagcgttt
     2761 acgcagcgcg tcgatcacat cagacgtgtc atccaccgac agcgtggttt gggtcataaa
     2821 ggagagcttc tcttcgtttt tgaccgtcag tttccacaca tcgtctggcg attcgaccag
     2881 atacattccc ccttccgggt tgctgtactg gcccatcgtc ccttccactt ccgggtgccc
     2941 ggcgtgaccg atgagaatag attcttcacc acgacggctg gcgcgggcga cttccatatg
     3001 cactttggtc accagcggac aggtcgcgtc aaacaccgtc aaatcgcggc ttttcgcttc
     3061 gttacgtact gcctgagaaa caccgtgtgc ggagaaaatc aggatcgcgc cgtccggtac
     3121 ttcgctaatc tgctcaataa agatagcccc acgctcgcgc aggctatcga ccacgtagcg
     3181 gttatgcacc acttcgtgac ggacatatat cggtgcgccg taaatggcca gcgcgttttc
     3241 aacaatgctg atagcgcggt ctaccccggc acaaaaacca cgcgggttgg ccaacaggat
     3301 ctgcatgtta cgcctccagt gccggatcga tttccagcac ttcaatatca aaatgaacgg
     3361 tctgcccggc cagcggatgg ttgaaatcaa cggtaatgga gtcgccgtta atttcgcgga
     3421 tcacgccagg catctcactg ccatccattg cggtaaaaag catgattgcg ccaatttctg
     3481 gctcgcctgc atccataaat tcacggcggg agaagtactg aatcaggtcc ggtgacggca
     3541 cgccaaacgc cgcatctggc tccagcgaga aggtggtttt atcgcccact ttcagcccca
     3601 gcaggtgttg ctccagccct tcagaaagag aagcatcacc caggcggaac agcgccggtt
     3661 taccgttgtt gcgggtagac tcagcagtgg tgccatcgtc gagttttagc gtgaagtgca
     3721 ccaggacggc gctattgctc tgtacagatt cagacatgca ggtttgctct tatattttga
     3781 ggatttttgc cggatgcgac ctacgatttc gcagcaatct gtaggccgga taagatgcgt
     3841 cagcatcgca tccggcaggg tttattattg ttttttcgct ttagaaggta aaaaaccttc
     3901 cagcacaatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga aggtggcgaa
     3961 gtgccagtcg ccgacataga agtcgatcat atcgacaacg aagccgtgcc acaggcggtc
     4021 gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt ttagcttctg
     4081 cgtggccttc gagcgataca tcatcactgc caggatcacg ctaataccaa tcgcaatacc
     4141 ggcaaaaaac caacgctgcc agccgccgct atcggccagg aaactaaacg ctgcgccata
     4201 gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc ccagagcaaa
     4261 gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta ccaccagcca
     4321 cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt acgtttttca
     4381 ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc cgccaccttg
     4441 ccgacatcct gggtgtagtg ccagcagcga gggcacttct caccttcggc tttactcaac
     4501 gcgactttca gccctttgag gacttcgctc tgctgggcat cagcaggtgc gtcgttatag
     4561 tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc cagcgcggtc
     4621 agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag cgagccgccc
     4681 actttcttat cggcacgcgc ttgctcaatg actttgttca cttcgccacg cactttcaac
     4741 agctcgtccc agaacgtatc gttcattgct tcactgtctg ccagaccaaa caggccttcg
     4801 taccactcgc cggtgaagac gtatttttca cgttcgcccg gcaggtaacc ccacacttca
     4861 tcagcggtga aggagaggat tggtgccatc cagcgaacca gcgcttctgc gatgtgatac
     4921 agcgctgtct ggcagctacg acgcgccacg ctgtccgctt tggcggtata ctgacggtct
     4981 ttgatgatgt cgaggtagaa ggaccccatc tcaacggagc agaagcgcat cagacgctgt
     5041 accacttcgt ggaaatcgta tgcttcgtac gctttgagga tgtcttcctg tgccgctttc
     5101 gcacaaccta cggcccagcg atccagtacc accatctctt ccggtttcac catatctttt
     5161 gccggatcaa aaccgttcag gtttgccagc aggaagcgcg cggtgttacg gatacgacga
     5221 tagctgtcgg cagcacgttt caggatctcg tcagaaacgg ccatttcgcc agtgtagtcg
     5281 gttgatgcca cccacagacg cagaatatcc gcacccagtt tattcatcac atcctgcggc
     5341 gaaacggtgt taccgatgga tttagacatc ttgcgaccct gaccatccac ggtaaagccg
     5401 tgagtcagta cctgacgata tggcgctttg cccttcatcg cggtagagat catcagggaa
     5461 gacatgaacc agccacggtg ttggtcagaa ccttccagat acatgtccgc tgcgtgaccg
     5521 gcaaattccg gacgcacatc aacaacggaa gagtgggtag atccggagtc aaaccataca
     5581 tccagcgtat ccggtacttt cacatactgg tcagcttcgt cgccgaggat ctctttcgca
     5641 tcgagatccc accacgcctg aatgccgtca acttcaacgc gttttgccac ttcttccatc
     5701 agttcgagag tacgcggatg cagttcttct gtgtctttgt gcacgaacag tgacatcggc
     5761 acgccccagg tacgctgacg agagatacac cagtcaggac ggttagcaac catcgactcg
     5821 atacgcgcct ggccccagtc agggatccac tgcacgcctt tgatctcttt cagtgactgc
     5881 gcacgcagac ctttctgatc catgctgacg aaccactgcg gcgtcgcgcg gaagatgatc
     5941 ggcgttttat gacgccagca gcacggatag ctgtgttgca ttttctcaac gtgcaacagt
     6001 gcgccttttt cctgcaacaa cgcaatgaca atatcgttcg ctttgaagac gttaacgcca
     6061 tccagagtcg ggtaagtacc cggcagataa gttccgtccg ggccaaccgg gttagcggtt
     6121 tccagaccat atttttgacc gatcacatag tcgtccggac cgtggcctgg cgcggtatga
     6181 acggcaccgg taccggcatc cagcgtaacg tggtcgccga gaattgccgg aacatcgaag
     6241 tccataaacg gatgggtaaa gcgcaacagt tccagctcgg cacctttcac cgtgccaaga
     6301 atggtgtaat cgctaacgcc gatacgctgc attacgcttt caaccagatc tttcgcgagg
     6361 atcacggcct gaccgtcgat ttgcaccagc gcataatcaa aatcaggtgc aatggagatt
     6421 gcgcggttag caggcagcgt ccacggcgtg gtggtccaga tcaccagcga gattgggccg
     6481 ttaacgttgc ttacggcaaa ttttgctttc agtgcatcct gatcgaccgc ctggaaagca
     6541 acgtcgatgg acggagaagt tttgtcgtaa tactcaactt ccgcttccgc cagcgcagaa
     6601 cggcagtcaa cgcaccagtg taccggcttc gcgcctttgt gcaggtgacc gttgccgatg
     6661 attttgccca gcgcgcggat gatattggct tcagttttga agtccatggt caggtacggg
     6721 tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc gtcaacctgg
     6781 gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtaaattt ctcacccggc
     6841 ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc ccagccaggc
     6901 acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc tttcagaatc
     6961 ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc atcatgcaga
     7021 atgaaggttt ttttgccttt tttagctgca cgaatgatgc cgtacagatc atcatcagtc
     7081 caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg gaaccctgtt
     7141 tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc gtatttcggt
     7201 ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg ggcggttaat
     7261 tcatcacgcg caatctgcgc tttcagttcg tccagcgatg caaatcgctg ctcattgcgt
     7321 atttttttac gcagcactac ttgtatatgg cgaccataaa ggtccattgc aacatctaac
     7381 aaatgcactt ccagttgctg gcgaataccg gcaaccgttg ggcgtgttcc gatgtttgca
     7441 acgccgggta acggcttttc gccaaggccc aacacttcta ccgcataaac ccctttcacc
     7501 ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt gcgccctaat
     7561 tcatcaccgt ggactacacg cccggagata gcaaacgggt gccccagtaa actttctgcc
     7621 agagccagat tgtcatccgc aagcgcctga cgcacggcgg tgctgctgat acgcacgcca
     7681 ccttcgcaaa aagtttgcgt gctggtgata tcgaagccgt attccatgcc cgctttctgt
     7741 aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcgtcacc tacggcaaga
     7801 aattttaccc gcaagtgctt caccagaaga tcactgatga agttttgcgc ggttaacgcc
     7861 gcaaaacgcc tgtcaaaacg cacgcacagc acgtaatcaa caccacactc tgcaagataa
     7921 cgcagttttt cccgcagccg ggtgagccgt gccggggctt tatcagtagc aaacagttcc
     7981 agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg cccttcttcc
     8041 tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt accaatagtc
     8101 agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat cagcttcatg
     8161 tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca aggttaaccg
     8221 gcgattgagt accgaaatca gaaagaagtg gcttttcatt gctatggcgc aaatcatggg
     8281 aagaaactga ccgcctgctg caatttttct cgcggaaaag ctgtattcac accccgcaag
     8341 ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg gcgcttattt
     8401 gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt tgaattgtcc
     8461 atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa gaagcgcgcc
     8521 attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat gcgtactttc
     8581 atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca gaaagcattt
     8641 aacgaaatgc aaccaatcgt ggaccgtcag gctgctaaag gtctgatcca caaaaacaaa
     8701 gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta attgcctgtt
     8761 gttgtcagct ttgtaaaaaa acccgctctg gcgggttttt ttatatccat ctgcaggcca
     8821 aaattcggcc tgcaaagtac aaatgtttat tttgaagctg gagtaaacag cgcagaatag
     8881 tctgtattgc agatacgctg tactgcaggg tgctgaatca tcctttcggc aaaaatcgcg
     8941 tggtactctt ccatcacgtt ctcaacacgg cctatctcca caaccgagtc atcgttatag
     9001 aaatcattag cgtaaagcga aggtgcaacg aaaatagcgt tatgcgtcgc cccaaaggct
     9061 ttcatcaacg cagcatcatc aaactcaccc aaaatttcga cgttcaagcc ctgggagtta
     9121 aaccagttta atagtttacg ccccaacatt gagcgacgcc ccggaataag taaacgacgc
     9181 tcttcaagac aggcaggaaa cggcttttct ggtagtgggt tagtgcacca gaaactgaca
     9241 ccacattcgc caattttcat ggaaaacagc ccttcctgct gagtggaatc gatcggacag
     9301 tcagagatga tcatatccag tttatgctga ctcaactgct ctaaaagcat ctcgtgcgtc
     9361 gattcgaaac agcgtagatg gatctgctcg tcttccacaa ctgcggcatc cagaacactg
     9421 ctgaccagac gtttggaaag tgcatctgcc acaccaacat caaacaataa gttggactct
     9481 ttgcgatagt tgacgatatc cagcatttcc tggcttaagg tgaacatttt atcggcatag
     9541 cgatagacca gttcccccag ttcgctgggt tccagaccac gtcctttacg cttaaatagt
     9601 ttcccttgca ggcgctcttc cagcgcccgg atctgcccgg taatggtttg tggtgttaaa
     9661 taaagcgcct ccgctgcgcc aaccacagaa ccttctttgt agacatgcca gaagtaatac
     9721 aagtggttgt aattgatatg agacatgctc atttctctcc ctgataacaa tgaaaaggga
     9781 gccgtttatg gctccccagt acatcgtcct gtcaaactga tggacgtaaa cgaacgcgta
     9841 accagctata tccaattacc gccgaagaaa ttgaaccgac aaggatacct aattttgccc
     9901 agttaatcag ttctggatct acgctaccaa atgccaggct ggcaataaag atagacatag
     9961 taaaaccgat accgcacagg ataccaaccg ccataatttg ctggtaagtc gttccctctg
    10021 gcagatgtgc caatttcaaa cgcagcgcca accagcagaa cagactaata cccagtggct
    10081 tgccaatcag caaaccggcg atgatcccta atggcagaat ggaggtcagg ccatccagcg
    10141 tcacgccttg cagagatacc cccgcattgg caaacgcaaa caacggcaaa atcagatatg
    10201 ccacccacgg atgcaaaaca tgctccagac gtttagccgg agagcgccca tgcttctctt
    10261 ttaacggaat aaagaagccg acaatgacgc cagccagcgt tgcatggaca cctgatttca
    10321 acaccgctgt ccacagtacc acgccaacca gaatataaac gcccgtgcgg cgtacaccac
    10381 acagattcaa taccgcgagt accgcaattg ctacagccgc gacgccaaga gaggccatcg
    10441 ataagtcatt agtgtagaac aatgcgatga taatgatggc cccaagatcg tcgataatag
    10501 ccagagccat caaaaagatc ttcagcgcta acggaacacg acttcccaac agcgccagca
    10561 caccaagtgc aaaggcaatg tcagtagccg ccgggatcgc ccacccttcg cgggtaatcg
    10621 gatcggcata gttaaaggcc agatagagca atgccggcac aatcatcccg ccgattgcgg
    10681 caataacagg aaatgccgcc tggcgcagac tggccagcga accttgcatc agctcgcgtt
    10741 taacttccag accaaccaac aggaaaaata ccgccatcag agcgtcattg atccatagca
    10801 gcatgttctt gttgatctca agtgtcccaa cccggagctg aaccggcgtc tcaagaaagt
    10861 cgtgatacca tccactggtt gcaccgctgt tggccataat catcgctaat acagcggcaa
    10921 taatgagaat tatgcctccc gaggcatcac tgctaaagaa tcgatgcaga tgtttcactt
    10981 tttatttctc ttcaggtgaa tagatcgttt tcacgatttt accccgcacg aatcatcgtt
    11041 aaaatcagat tatagtgtag gaatagctcg ctttttacga tctattaaaa gaattgcgcc
    11101 atcagcgaga gatgagtttt tgatgcccga aagcggcggc tgatgacctt aaaccccatt
    11161 aatcatagtc taatgtcaag atttctgaag aaattattcg gaaaagtaat aattaaattg
    11221 ttccatgctc tagtttttta ttccacagat gcttgaggat atctaatgta gaaacgacat
    11281 tcgcaggaga cattcggttc aatgatataa ggaaaggatg gttctccaca aagtggataa
    11341 aacgcgtcgc actgttcaat gttgcatgcc gcatctcgcg gactttctta ctgccagtga
    11401 gattgcatac agccattgaa taattgcggt tagaaaatgc acgccagaca ttatttcgta
    11461 ccccaccact aaggaataat cgcggtggaa aatcaatggt ggcgggtgcc acaccgcgct
    11521 ctttgacaaa ggaaatatgg cagcgacctt gatgaatatg ctgcaaatta gccatcaggc
    11581 agccgcccaa tataaagcat tcgtgatcaa ggtaatgctg aacgttgttg gaatgctcat
    11641 cggtcagtat tctgaccagt tcaaaatgac gatatgccgg aagaaccggt tttaccaaca
    11701 ttcccggatc gttttgatgt aaaactgccg ggccgtaatc aatatcaaac aaggtatccc
    11761 gccgcatagt aagtaactct ttgcgctgga catgatgtgc cagcgtccct ggagacatac
    11821 gctcttcata gttcgatgtg tattgataat gttgctctac ggcagatggg gagtaattgg
    11881 ttgagatggc aaccaccctg ccgctttgtt cttctgcggt aacaagagca taaagagagt
    11941 tattgctacc attatacttg acgcgaaaag cgcgggtact cagggtaata tcagaagctg
    12001 ggaaacactg tcgactttct ttatagtttg cttcacgcgc taattttaac agttcacggc
    12061 taagtccagt gctgttaaga agtaatgact ttctgatatc cgcaagtgcc tccccttgct
    12121 caatcatcaa tgctaactgg gctcctcgtg gtgtggtaat tacatgttcc agagtgataa
    12181 atgttttctc acaatgatgg caatacattc ttctctggcc ttgtgcagaa tatccatatt
    12241 ttttgaggga cgtgcctccg caagttgaac attggcaaat caaacctctc caggagtgat
    12301 tcacaatatt acgataaata ttaagcgact gttcagatat cactggaaac aagtaaccac
    12361 attcacggca taaaatattt ttaccctgtg cgacataatc ctggctattc agcaacccca
    12421 ggtttttaca tcctggtgtt ttgcaacaat caacgtttac gttggtaaac atatccactc
    12481 cctgcgaata gccgtatctt gatattgttc aacgcgaaaa gaacctgatt caatgcgtag
    12541 cactgaatca ggcgggcata gccctaaatg gcatacatta catttattat gattatcagg
    12601 ttagccccag gtcaccgctt ctggtttatc acctctggct ttcattagag aatcattatc
    12661 aatatcagtt ttatatttta aaaacttacc atacatctgt ttttcaatat ctggtttttt
    12721 accgatcaga ttaagtgttt cataacgatc agatttcaga ttgtagagat atttcggttt
    12781 attattgcga tcgataatca tcttccagtc accatcacgg atcgcccatt catcggttgg
    12841 atcatcctgg aatggcatat caatcccgaa aattaatggc ttttcgcgtt tcaatgcttt
    12901 ttgctcaaga acaggaacca gcgattcacc atcgaaagta cggtctgtag gtaatttgaa
    12961 gttcatcatt ttcgctaaag taggcatcca gtccagacca taaacgggtg tatctgaaac
    13021 cattccctgt ggtagatgtt taccatattt aataatggct ggaacacgaa ttccgccttc
    13081 ccaaaggtta tccttgcgac cgcgtaatcc atccgtttcc cctgccaaat tcagctcata
    13141 cactttgcgc gcttcacgcg ttaccggacc gttatcactg gtaaaaataa cgattgtgtt
    13201 atcttcttca cccatcgctt tgattttatc cagcactttt ccaacctgtg catccagata
    13261 gctgatattg gcataatatt cccccacacc acgccagggt ttgtctgccc agtcgccata
    13321 aaataaatca ggatgctgct tctgatacgc gctcatatat tgtgagtaca tatcgaggta
    13381 ttttttgggc gaagccaggg ggctatgcac ttcggtaaaa gcaacataga ggaagaaagg
    13441 cttgctgtcc tttttgttat ccagccagtt gacgacttcc gaactgacat actcaccgct
    13501 cattttatca gctcgtggag tgggttgccc gttacgtagc cagcctgtcg ggtaaaccat
    13561 gccataacgc gggcgttctt tagcgttatc cagcgtggcg tcggtaacga agcccgccgt
    13621 attaaccagt gagtaatcaa agcccatatc ttgtgcttgt ggctgatcgg tgcgatcgcc
    13681 gcctgcattc agatgcagct tacccatcat tgccgtgtcg tacccttgcg ctttgagtag
    13741 attagcaatc gtgagttcgt tacgccctaa ggcaacatct ttgcctgaag gaatccatga
    13801 gcgaattcca gtacgaaatg gcatccggcc ggttaatagc cctgcgcgtg aaggtgaact
    13861 taaaggagcg ggggcatagt agtcagtaaa tttgacccct tcctgggcaa gcctgtcgat
    13921 attaggtgtt ttaacgatct gatgaccata tgttgctaaa tcgccataac ctaaatcatc
    13981 cgccataata atgactaaat taggttgttt agcctctgcg gctaaggcag gactaaaagc
    14041 attccctgtg caaactgcaa ggccgatcaa actggccatt aacgttttct gcataattat
    14101 tccctgataa aaaattagaa atcataacca agcatataaa tcatttgatc gccaaagcca
    14161 tcgtagccca gcttattatc gaaataacgc cacgtcacac tggctttcca gcgaggatag
    14221 aacttccagg caatagttaa accgccattt aacccgttcc ggccaaattg ctgctccgtg
    14281 taagcatcgt tcctgtccag ttctatttca ttccagttag ataaaacaaa tttttcgtca
    14341 aataacgtaa atggtaatac tgctgtccag ccaacaacat aaccattcca accattcgtt
    14401 tgaccatatt tagctgatac gtagtcacca gattgattat gcaatccaat atacggttta
    14461 aaaaagcccc actcgccgtt ccagccgagg taaccgaatc cataaaacat gtccagatcg
    14521 tcaccccatg agttatccca ttggccataa attttgccaa agaatgtcat atcagagtca
    14581 aagagtctga catgcgtcat tgctgaaacc gtatgatttc tccctgctac agcatgattg
    14641 agtacattac tttcataaaa tgagtaaaat tctgcttctt taaaatttac cccaaaatcc
    14701 gcagtcactt tccatacttc accacggtga gtgtttacaa atccactatt ccagtctgca
    14761 taacccgcat ttattgaacc aaaggttcct ttatattcta atgaagaata tgcaggaaaa
    14821 tgatagatag ctgttatcag ggtacacgca ataagaagtc gtcttttggc agacatataa
    14881 tatgttctcc attcattgtt tttatgattc cataggaatt aatacaccag acaattcatt
    14941 tttattaaga atgaccaggc cagtatattt aaagatgact atcgaagcgt gatgaagatc
    15001 gcagattact tttgcgaacg ggcggaggga tatcgtgttt cagaaaatgt gtctgtttta
    15061 aacatcaccc tttgatttat aggcattttt catgatgaac ttagcattgg ctgctgaacg
    15121 tatttctttg tctctctata gtattacagt aacctttaat aattaatttc ctttagccat
    15181 tcacttttga tagaggaaaa cacctttttt tgtgaatccc tggatatatt taagttaaat
    15241 aaattgatgt attgaaatat tattccgcaa aaatgcgagg cgtcttcaga aaaagaattg
    15301 ataaaacaga agtgaattaa aagcgcagta taatagaggc gggtgcttga ggctgtctgt
    15361 ctcaggcatt ggctgaacgg cagatagaga aaagccccga gtgatatttt accatcaacc
    15421 cgaggccccc tatatgcaga acacatgtag agtgcctctt actgaccgaa aggtcaagga
    15481 gaagagagca atgaagcagc ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac
    15541 cgccgtagtg gcggcgctgg taacgagaaa agacctctgt gaggttcaca tccgaactgg
    15601 ccagacggag gttgctgttt tcacggctta cgaatccgag taagagcaac ggcggggagt
    15661 gatccccgcc actttacagg tgctcgcata tcttcaacgc accctggttt ttaccctggc
    15721 ctgcccacgg gcaggctttt ggggaggtta gcgagtcagg tcgtcaaaaa acttcttcac
    15781 gccatcaaag aagctctttg aacgcgggct gttgtgctcg ccggttgggc caccaaagct
    15841 ttcttgcagc tcttgcagca gctgtttctg cttctcgttc aaacctaccg gtgtttcgac
    15901 aacaacgcgg catagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc
    15961 gcgcatacgg aacagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc
    16021 atcaagggtc ggtacttcga tttcaccacc cagcgccgcc atagcgaagt tgatcgggac
    16081 ttcgcaatac aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg
    16141 aacgtacaga tcgcctgccg gtgcgccgtg ttcacccgct tcaccttcgc ccgcaagacg
    16201 gatgcggtct ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc
    16261 aacacgacca tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg
    16321 acagtgtgga caggtctgct gcacggcaaa gaaaccctgg cgcatctgca cctggccaga
    16381 accatgacag gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca
    16441 aacgtcacac tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc
    16501 ttcgagggtg agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc
    16561 acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc
    16621 gccgccaaaa ccgccgccgc ccatgccgcc ttgctcaaac gcagcatgac catactgatc
    16681 gtatgccgca cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa
    16741 tttcgcctcg gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg
    16801 tttgtaggcc tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta
    16861 ataatcttgc ttagccatct tttttgattt gcccctagat gaatgcacgg gcggagagga
    16921 aattcccctt cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt
    16981 tgacttcttc aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat
    17041 cagcaccggc agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg
    17101 aaacctgtgc cagttcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag
    17161 cagtttccag tgcagtcagc gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt
    17221 cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag
    17281 tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca
    17341 ttttctggat ttcatcttcg ttcagaccag aagaagcctt gatagtgatc ttctgctctt
    17401 taccgctgtt tttgtctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg
    17461 taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac
    17521 ccagagattt gttatcagcc gcacgtttac gttcaccctg cagcacatgg atggttaccg
    17581 cagactggtt gtcttcagcg gtagagaaca cctggctgtg cttggtcggg atagtggtgt
    17641 ttttcgcgat cagcgtggtc atcacaccgc ccatggtttc gatacccaga gacagcgggg
    17701 taacgtccag cagcagtacg tcttttacgt caccagtcag aacaccaccc tgaacagcag
    17761 caccgatggc tacagcttcg tccgggttaa catctttacg cggctcttta ccaaagaatt
    17821 cagcaacttt cttctgaacc attggcatac gagtctgacc accaacgaga ataacgtcgt
    17881 cgatatcaga tacggacagg ccagcgtcct gcagtgcaac tttcagcggc tcaatggaac
    17941 ggtttaccag atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt
    18001 gtttcggacc ggtcgcgtct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcag
    18061 aagacagttc gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat
    18121 cgttgcgcag gtcaatgccc tgatctttct tgaattcttc aaccagatag ttgatcagac
    18181 ggctgtcgaa gtcttcacca cccaggtggg tatcaccgtt ggttgccaga acttcgaagg
    18241 ttttttcgcc gtcaacctcg tcgatttcga taatggaaat atcgaaagta ccaccaccca
    18301 ggtcataaac cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag
    18361 ctgcggtcgg ttcgttgatg atacgtttta cttccagacc agcgatacgg cctgcgtctt
    18421 tggttgcctg acgctgagca tcgttaaagt atgccggtac ggtaataaca gcttcagtta
    18481 ccggttcacc caggtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaga
    18541 tctgcggcgg tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag
    18601 caataatttt gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc
    18661 ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt
    18721 tagccggctg accaaccaga gtttcaccat cctgggtata ggcaatgata gaaggcgtgg
    18781 tgcgatcgcc ttcggcgttc tccagtacac gaggagtggt gccatccata atcgctacac
    18841 aagagttggt agtacccagg tcgataccaa ttattttacc catctaaacg tctccactat
    18901 atattcggtc atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg
    18961 cccaatttca tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac
    19021 ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat
    19081 atggcaggac aagacgtgaa actgacaaca attttattca tataaagaat gaatcgcagc
    19141 caaggcattc atcaaaaaat tttaataaaa agaaaatatt acgtacctga aacttctctt
    19201 tatcagcagt aaactagttt catcccctta cccctcccca ccaagagaat ccttatgaaa
    19261 tccgttatta cgatttccgc cagcctggcg attagcctga tgctgtgctg cacggcgcag
    19321 gcaaacgacc ataaaatcct cggcgtcatt gcaatgccgc gaaacgaaac caacgatctg
    19381 gcgctgaaac ttcctgtttg tcgcattgtt aaacgcatac agctctcagc cgaccatggc
    19441 gatttgcagt taagcggtgc atcgatttat ttcaaagcca cccgaagcgc cagtcagacc
    19501 ctgaatattc cttcagaaat aaaagaaggg caaaccactg actggatcaa cattaacagc
    19561 gataacgaca acaaacgctg cgtctcaaaa atcacctttt ccggtcatac agtgaactca
    19621 tcggatatgg ccacgctgaa aattatcggc gacgactaac gccagaatat agaaaccaca
    19681 aaaaatgaat gttaattacc tgaatgattc agatctggat tttctccagc attgtagtga
    19741 ggaacagttg gcaaatttcg cccggctgct cacccataat gaaaaaggca aaactcgcct
    19801 ctccagcgta ctgatgcgca acgaactgtt taaatcgatg gaaggacatc ccgagcgaca
    19861 ccgccgcaac tggcagctga ttgccggaga attacagcat tttggtggcg atagtatcgc
    19921 caacaaactg cgcggacacg gtaaattgta tcgcgccatt ttgctcgatg tttcaaagcg
    19981 attgaagctg aaagccgaca aagagatgtc tacgtttgaa attgagcaac agttactgga
    20041 acaatttctg cgtaatacct ggaagaaaat ggacgaggaa cataagcagg agtttctgca
    20101 cgcggtcgat gccagggtga atgagctgga agagctgctg ccgctgctga tgaaagacaa
    20161 attattggca aaaggtgtgt cgcatttgct ttccagccaa ttgacccgca ttttacgcac
    20221 ccacgcagca atgagcgtgc ttgggcatgg tttgctgcgc ggcgcgggtc tgggaggccc
    20281 tgtgggcgca gcattaaatg gggttaaagc ggtcagcggc agtgcctatc gcgtgacaat
    20341 tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt agcgccactc aggtctgata
    20401 ccggaaaccg gggaaaattt tcattttccc ctcaaaagat cgtagacact gccccactgg
    20461 ctgattatta tgccgcgccc tgaaaacact acagttattc agggaaatta tttcaccatt
    20521 cattcgatga tgatttttga ggaattatgg gcaacactaa gttggctaat ccggcaccgc
    20581 tgggcctgat aggcttcggc atgaccacca ttctgcttaa cctgcacaac gtgggttatt
    20641 ttgctctgga cggtattatt cttgccatgg gcattttcta cggcggcatc gcgcaaattt
    20701 ttgccggtct gctggagtac aaaaaaggca acactttcgg tttaaccgca ttcacctctt
    20761 acggttcttt ctggctgacg ctggttgcaa ttctgctgat gccgaaactg gggctgaccg
    20821 atgcgccaaa tgcccagttc cttggtgtct acctgggtct gtggggcata tttacgctgt
    20881 ttatgttctt cggcacgctg aaaggcgcac gcgttctgca attcgttttc tttagcctga
    20941 ccgtgctgtt tgccctgctg gcgatcggta acattgccgg taacgccgcg atcatccact
    21001 ttgccggctg gattggtctt atctgcggtg ccagcgcaat ctatctggcg atgggtgaag
    21061 tgctgaacga gcagtttggt cgcaccgttc tgccgattgg tgagtctcac taattgttaa
    21121 gctccccccg ctcgaaggga taagattgtt aacaacgtgc gctttgttt
//
LOCUS       DAZKHK010000092        21084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKHK010000092 DAZKHK010000000
VERSION     DAZKHK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.092"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..189
                     /locus_tag="ACHOS5_003459"
                     /pseudo
     CDS             1..189
                     /locus_tag="ACHOS5_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            404..1522
                     /locus_tag="ACHOS5_003460"
     CDS             404..1522
                     /locus_tag="ACHOS5_003460"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP9778222.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERSFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            1588..1836
                     /locus_tag="ACHOS5_003461"
     CDS             1588..1836
                     /locus_tag="ACHOS5_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP9778223.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1901..2269
                     /locus_tag="ACHOS5_003462"
     CDS             1901..2269
                     /locus_tag="ACHOS5_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9778224.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2363..3016
                     /gene="nfsB"
                     /locus_tag="ACHOS5_003463"
     CDS             2363..3016
                     /gene="nfsB"
                     /locus_tag="ACHOS5_003463"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP9778225.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3124..4371
                     /gene="ybdG"
                     /locus_tag="ACHOS5_003464"
     CDS             3124..4371
                     /gene="ybdG"
                     /locus_tag="ACHOS5_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP9778226.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4528..5910)
                     /gene="pheP"
                     /locus_tag="ACHOS5_003465"
     CDS             complement(4528..5910)
                     /gene="pheP"
                     /locus_tag="ACHOS5_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP9778227.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(6012..9155)
                     /gene="cusA"
                     /locus_tag="ACHOS5_003466"
     CDS             complement(6012..9155)
                     /gene="cusA"
                     /locus_tag="ACHOS5_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP9778228.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVIIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9167..10390)
                     /gene="cusB"
                     /locus_tag="ACHOS5_003467"
     CDS             complement(9167..10390)
                     /gene="cusB"
                     /locus_tag="ACHOS5_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP9778229.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTCFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10406..10738)
                     /gene="cusF"
                     /locus_tag="ACHOS5_003468"
     CDS             complement(10406..10738)
                     /gene="cusF"
                     /locus_tag="ACHOS5_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP9778230.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10762..12144)
                     /gene="cusC"
                     /locus_tag="ACHOS5_003469"
     CDS             complement(10762..12144)
                     /gene="cusC"
                     /locus_tag="ACHOS5_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP9778231.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12301..12984
                     /gene="cusR"
                     /locus_tag="ACHOS5_003470"
     CDS             12301..12984
                     /gene="cusR"
                     /locus_tag="ACHOS5_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP9778232.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12974..14416
                     /gene="cusS"
                     /locus_tag="ACHOS5_003471"
     CDS             12974..14416
                     /gene="cusS"
                     /locus_tag="ACHOS5_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP9778233.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TEETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDVRGTKFVIILPA"
     gene            14566..16803
                     /gene="nrfB"
                     /locus_tag="ACHOS5_003472"
     CDS             14566..16803
                     /gene="nrfB"
                     /locus_tag="ACHOS5_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP9778234.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            16790..19762
                     /gene="nfrA"
                     /locus_tag="ACHOS5_003473"
     CDS             16790..19762
                     /gene="nfrA"
                     /locus_tag="ACHOS5_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP9778235.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFAHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYGA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            19763..20653
                     /locus_tag="ACHOS5_003474"
     CDS             19763..20653
                     /locus_tag="ACHOS5_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP9778236.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
BASE COUNT         5072 a         5676 c         5304 g         5032 t
ORIGIN      
        1 ggtttggagt ggcagcccca gggttgatag aaatatctcc tggggcttca tctttccaga
       61 cctcagagta aacctgaaac cggaaagctt caggcacccg ccgttatctt acccttcctt
      121 agcaaaatag ctgtttattt atacagtaaa tcctgtttcg gacttctcac tcctcattta
      181 atcaactgaa gctgaacccg tttttttctg catgcctcaa tcgtcaaaat tcttcgccgc
      241 cagccagctc tgccataact tgtcacattg caaaagtagc caaattgctt cagtcacaat
      301 cagttctcca tcaccaaaaa aagaaacatt ccagaacttc cgcatctcac ccagccgctc
      361 ccacttctat actgaataga aaacgccaac ataagagaaa cctatgccat tacccgattt
      421 tcatgtttct gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc
      481 gggctatgac ttaagccagg actcatcaat gctgattgac acggttaaaa atcagatcac
      541 ggccggagag gtaaagcacg atataaccga aagtatgctg gagctggcga cggatgtttg
      601 ccgtgatatc aaccaggctg ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc
      661 agcggcagac catcatctgg aaatttgcgg cggtggcacg catccgtttc agaaatggca
      721 gcgtcaggag gtatgcgata acgaacgcta tcaacgaacg ctggaaaact ttggctatct
      781 catccagcag gcgaccgttt ttggtcagca tgtccatgtt ggctgcgcca gtggcgatga
      841 cgccatttat ttgctgcacg gcttgtcacg gtttgtgccg cactttatcg ccctttccgc
      901 cgcgtcgcca tatatgcagg gaacggatac gcgttttgcc tcctcacgac cgaatatttt
      961 ttccgccttt cccgataatg gcccaatgcc gtgggtcagt aactggcaac aatttgaagc
     1021 cctgtttcgc tgcctgagtt acaccacgat gatcgacagc attaaagatc tgcactggga
     1081 tattcgcccc agcccccatt ttggtacggt ggaagttcgg gtgatggata ccccgttaac
     1141 ccttagccac gcggtaaata tggcgggatt aattcaggcc accgcccact ggttactgac
     1201 ggaacgctcg tttaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca
     1261 ggcctgtcgc tatgggctgg aaggcgtcat taccgatccg catactggcg atcgtcggtc
     1321 actaacggaa gccaccttgc gtttactgga aaaaatcgcc ccttctgcac ataaaattgg
     1381 cgcatcgagc gcaattgaag cactgcaccg tcaggtcgtc agtggtctga atgaagcgca
     1441 gctaatgcgt gatttcgtcg ccgatggtgg ctcgctaatt gggctggtga aaaagcattg
     1501 tgagatctgg gccggagaat aagtcgggaa ttgccttaca gaagaatatc cctgacaatg
     1561 gtgttttaac ttacatttaa caatgttatg aaacaccctt tagaaacctt gaccaccgca
     1621 gcaggcattt tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg
     1681 ctgacgctgg cacaaaaact ggtgaccacg ttccatctga tggatcttag ccagctttac
     1741 actttattgt tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctacgc
     1801 tttatctggc gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc
     1861 ggcatacttt gcttattctc cttcgcctaa aggaacattt atggataagc aatcactgca
     1921 cgaaacggcg aaacgcctgg ctcttgagtt accctttgtc gagctttgct ggccttttgg
     1981 cccggagttc gatgttttta aaattggcgg caagattttt atgctgtcgt cggagctgcg
     2041 cggcgtcccc tttatcaatc tgaagtccga tccacaaaaa tccctgttaa atcagcagat
     2101 atacccgagc attaagccag ggtatcacat gaataaaaag cactggattt cagtgtatcc
     2161 cggcgaggaa atctccgaag cgttacttcg cgatctgatc aacgattcgt ggaatctggt
     2221 ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct
     2281 atagcgcatt tttctcgctt accatttttc gttgaacctt gtaatctgct ggcacgcaaa
     2341 attactttca catggagtct ttatggatat catctctgtc gccttaaagc gtcattccac
     2401 taaggcattt gatgccagca aaaaactaac cccggaacag gccgagcaga tcaaaactct
     2461 cctgcaatac agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac
     2521 ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccgat aattacgtgt tcaacgagcg
     2581 taaaatgctt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc
     2641 ctggctgaag ctggttgttg accaggaaga tgccgatggc cgctttgcca cgccggaagc
     2701 gaaagccgcg aacgataaag gtcgcaagtt cttcgccgat atgcaccgta aagatctgca
     2761 tgatgatgca gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctggg
     2821 cgtggcggcg ctgggtctgg acgcggtgcc catcgaaggt tttgacgccg ccatcctcga
     2881 tgaagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtgggtca
     2941 tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacattac
     3001 cttaaccgaa gtgtaattct cccttgccgg gcatctgccc ggctatttcc cctcagattc
     3061 tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac
     3121 gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat tgatcacacc
     3181 acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattttt
     3241 ttgcactggg tggtactgcg gaccttcgaa aaacgcgcca tcgccagttc acggctatgg
     3301 ttgcaaatca ttacccagaa taaactcttc catcgtttag cttttaccct gcaggggatt
     3361 atcgtcaata ttcaggcggt attctggcta caaaaaggca ccgaagcggc agatattctg
     3421 actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg
     3481 gatgtgattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg
     3541 atttttcagg ggattaaact gatcggcgcg attctggtcg gcattttgat gatctcgctg
     3601 ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgcta
     3661 atgttggtat ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac
     3721 gatatgctga aactgggtga ctggctggag atgccgaaat acggcgcgga tggcgcggtg
     3781 atcgatattg ggttaaccac cgtcaaagtt cgtaactggg acaataccat taccactatt
     3841 cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc
     3901 ggacgacgta ttaaacgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa
     3961 gacgaaatgc aacgtctgaa taaagcgcat ttgttaaagc cttatttaac cagccgccat
     4021 caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc
     4081 cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg gaatcatccg
     4141 cggattcgca aagatatgac cttaatggta cgccaactgg ctcctggtga taacggttta
     4201 ccgcttgaga tctatgcgtt taccaacacc gtggtgtggc tggaatatga aagcattcag
     4261 gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag
     4321 tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa
     4381 ccgctctcat cgaatggatg agagcggttt cggatggttg acatcgtttt gtcggatgcg
     4441 gcgtgaacgc cttatccgac ctacagaatc gttacaaatt gtaggcccga taagcttgcg
     4501 catcgggcaa atcagcctca catcagctta gttcactttc ctacgcaatg ttttaaacgc
     4561 cacaaacagg aatacaatcc acaccggcag cagaatcgct gacaagcgca tatcatccat
     4621 cgtgcacatc agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg
     4681 atagagcagc gccttaaatt gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg
     4741 cagatgcgcc aggcagatca taatccagtt caacagcagc gttgctacca ccagcgccat
     4801 cagcagacca aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat
     4861 cgctccggaa agcatcagcg agttaatcgg cacgccgcga cggctgacgc gagtcaaaaa
     4921 ctttggcgca ttgccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc
     4981 gctgttatac accgacagcg atgccaccag aatgacgaag ttcagcgcag aagccaccac
     5041 attgctgtcg agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac
     5101 ccacgggtag agcgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata
     5161 caccacctga ttcactgctt tagggatgct tttttccgga tcgcgcgctt cagccgcagt
     5221 aatcccaatc agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag
     5281 tccattccag ccggtggcaa agaaaccgcc gtagcgccag aggttatcga tactggcttt
     5341 ctcaccgccg tgcccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc
     5401 cagcacttta atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag
     5461 gttaacggcg ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg
     5521 gaaccagtac tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa
     5581 cattacccag tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc
     5641 aaagtgggca aatgaaccgg ataccggctc ttcgaccacc atctcgccga gctggcgcat
     5701 aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat
     5761 ctgaatcgcc gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat
     5821 cagttgaata tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt
     5881 atcttccgat acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag
     5941 gggcaccttt taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacggg
     6001 gtatcctgct tttatttccg tacccgatgt cggtgcagcc acatcagctt atacgccgcc
     6061 gggataataa acagcgacag caaaggcgcg gtgatcatgc cgccaatcat cggcgcggca
     6121 atccggctca tcacctctga ccctgctcct gttccccaca gaatcggcag cagaccagcg
     6181 ataatcaccg ccaccgtcat cgcttttggg cgcacgcgca gtaccgcgcc gtgatataac
     6241 gcctcatcca gcttctgctc gctgaatgtt tgcggattat tcaacgacgg ctcggcctct
     6301 atggcgtgac gtaaatacat cagcatcacc acgccaaatt cggcggcgac cccggcaagg
     6361 gcaataaaac cggtgcccgt cgccacggaa agatgaaagc ccatccacca cagcaaccag
     6421 atgccgccca ccagcgcaaa cggtacgctg ctgataatca gcaacgcttc gcccacccga
     6481 cggaacgcca gatacaacag cacgaagatg atcatcagcg tcatcggcac catcagtttc
     6541 agcttatggt tggcgcgctc cagtagctcg aactgcccgg agaatgccac gctggtgccc
     6601 ggttttaact gcactttttc agctatcgct ttttgcagat cgtgaaccac cgacaccatg
     6661 tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc gcgcattctc ggttttcagc
     6721 atcgacggtc cggtagagac tttaacgtcg gccacgtctg ccagggtgat ttgctgcttc
     6781 atcggcgtca ggatcggcaa ctggcgcagt gcctgcgggc tatcgcgcca gctttgcgga
     6841 taacgcagat taattggata acgagcaatc ccttccaccg tttcgccaac catcgccccg
     6901 cccaccgcag aagtcacaaa caactgcaca tccgccaccg tcataccgta acgcgcggct
     6961 ttttcacggt taatctcaac gttgatatag cgcccacctt ccagacgctc agcaagcgca
     7021 gaagccacgc ctggcaccgt tcgcgccact tcttcaattt gctccgccat cgtgtcgata
     7081 tccgccagca cagtgccgga aactttaatg ccgatagggc ttttaatgcc agtcgagagc
     7141 atatcgatac ggttacgaat tggcggcacc cacagattcg ccagccccgg cagacgcacg
     7201 gtgttatcca gttcctcaat gattttgtcc atcgtcatgc caggccgcca ctgatcctgc
     7261 ggcttaagct ggatagtcgt ttctaccatc tccagtggag cagaatcggt ggcggtttcc
     7321 gctttcccgg ttttgccaaa tacccgcgcc acttcaggta cgctcataat cagcttgtcg
     7381 gttttttgca gcatactcgc cgcctccgct gcggaaatcc ccggcagcgt cgatggcata
     7441 tacaacaagt cgccttcatt gatctgcggt aaaaattccc cgccaacttt attgagcggc
     7501 cagagaaccg tcagcaccga aagcgctgcc accaacagcg tggttttcgg ccagtgcagt
     7561 actttcagca acagcggatg ataaacacga atcaaaaagc gattgagcgg gttactgctt
     7621 tctggcggaa ttttgccacg gatccagtag cccatcagga tcgggatcac tacgatcgcc
     7681 agcagcgccg cgcccgccat cgcataggtt ttggtgaacg ccagcgggcc aaacagacgc
     7741 ccctcctgcc cttccagggt gaaaatcggg ataaacgaca gcgtgataat cagcagactg
     7801 ataaacagcg ccggccccac ttcaacagat gcatcggtga tcacctgcca gcgcgtttta
     7861 ttatccagcg tggcgtcagg atgctggtgc tgccactctt ccagccgttt atgcgcattc
     7921 tcgatcatga cgatagcggc atcgaccatc gccccgacgg caatcgcaat gccacccaac
     7981 gacataatat tggcgttcag tccctgaaag tgcatgacaa taaaagcaat acacaacccc
     8041 agcggcaacg aaataatcgc caccagcgcc gagcgcacat gccagagaaa cagcgcacag
     8101 actaccgcca ccacaataaa ctcttccagc aacttgccgc tgaggttgtc aatggcgcgg
     8161 tcaatgagct gactgcgatc gtatgttgta actatctcca cgccttccgg cagactgctt
     8221 ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt cacgggcgtt tttgccggaa
     8281 cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt tcagttcggc aatgccccgg
     8341 cgcatctccg ggccaacctg gaccttcgca acatcacgca gataaacggg cacgccattt
     8401 tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg tttgcagata gccgctggcg
     8461 cgcaccatat attccgcttc cgccagttcg atcgacgaac cgcccgcttc ctggtttgaa
     8521 gcatccagcg cgctttttac ttcggcgaga ctgatgccat actgcgccag gcgctgagga
     8581 tcgataatca cctgatactc tttcaccaca ccgcccaccg acgccacttc cgcaacgtca
     8641 gggatggttt ttagctcata tttgagaaac cagtcctgca atgagcgtaa atcggccaga
     8701 tcgtgcttac cgctgcgatc caccagtgca tattcataga tccagccaac acccgtggca
     8761 tctggcccca gctcggcgct gactcccgca ggcagcttac cctgtacctg gttgaggtac
     8821 tccagcaccc gcgagcgcgc ccagtacgga tcggtgccat cttcgaaaat gacatacaca
     8881 taagagtcgc caaactgcga gaaaccgcgc acagtcttcg cgccaggcac cgacaacatg
     8941 gtggtggtta gcggataagt cacctgattt tcaacgattt gcggtgcctg accgggatag
     9001 ctggttttaa taatcacctg cacatcggaa agatccggca gtgcatccac tggcgtgtta
     9061 atgatagtcc aggtgcccca gatactcaga aacaacgcgc ccatcagcac cagaaaacgg
     9121 ttcgccaccg agcgacgaat aatccattca atcattggct attccctcaa tgcgcatggg
     9181 tagcactttc agagcgcatc cgctccagtg cgccagaaat attggcttca gaatcaatca
     9241 ggaacaggcc gctggaaacc accttttcac cttccgccag accagagcgt aacgcggtga
     9301 cgccttgcga tgcctggaaa acggcaacgc gtttcggtac aaagcgcccg tcagcatcaa
     9361 cggtaatcac ccgctgttcg ttgccggtat caatcagcgc ttgtgacgga atgagcagca
     9421 tcggttcgct ggcggtattg agttgcaacc aggcgttcat gcccggtttt agcgcctcgt
     9481 cggcgttgtc gacttccaga cgcagttgca gcgtgcgggt cgcagcatcc acgccaggta
     9541 gcagcgtcca tttgcggatg gtgagtgttt tatcaggtcg cgccggaacg gtgagcgtaa
     9601 actgcgaggc atctttcacc agccaggcga tggactccgg gatcgcagca gtgacccaca
     9661 ccgggtccat accctgaatt ttcgcgacca cgttatcttt ggcgatattc attcccgcgc
     9721 gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt gagcgtaaag caagtctgga
     9781 ttttttgcgt ggcgatcaga cggcgaatat cagcctccgg cattcccgcc agtcgcagtc
     9841 gctcaagaat gccttcagtc tgggtcgccg taccgccggt ttcgcgcagc agtaaatact
     9901 cactctgcgc ttccacccag tcgggaatgg tcaggtcgag aagcggcgcg cccttttgca
     9961 ctttatcgcc cacggtaagc ggatacacct tgtcgataaa cccggcggcg cgggcctgca
    10021 caatggcata ctgatactcg ttgtaactga cattcgccgg gaaactctgg gaaaaagtca
    10081 gcggcccgcg cgtgacggtc gccgttttca cccccagatt ctgcgtctga gtcgggtcaa
    10141 tgcgcacacc agacgcagaa ctctcttcat cggcatattt cggcaccaga tccatatcca
    10201 taaacggcga tttccctggt ttatcgaacc gcgtattggg atacatcggg tcgtaccaga
    10261 ataagacttt acgttctgcg gtcgacgttt tttctgcggg cggttcctcc tttgcaaacc
    10321 aggtaaaacc tgccgcagaa ataataccgc ccgcgatcat gctgccgata ataagcgcga
    10381 tttttttcat ctcattaaac ccggattact ggctgacttt aatatcctgt aataaagaaa
    10441 ggttgccctg ttggacaaaa ttaaacgcta ctttatcgcc ggttttaatt tcgctcattt
    10501 tcgtctgcgg ggtgatggta aagcgcatag tcatctccgg ccagttcacg gcagcaatcg
    10561 gatcgtgatg gatggtgatt tttttgcttt ccagatcaac accttttacc acgccagtgg
    10621 cgctaataac ctgtggttgt gcttcgctca tggtttcatg atgatgttcg ttagcctggg
    10681 cattaaagcc aataacggta aacagactaa acattgcgac ttgcagtgct tttttcattt
    10741 attctctcct ggagttaaaa attattgctg ccaaccgccc cctaaggcgg tatataaaga
    10801 aatttcgtta acctgacggg cataattcag gtcaagtaaa gtttgtcggg ttgcaaataa
    10861 agaacgctcg gcatccagca cttccagata acttaccgcg ccgtgctgat ataatgcccg
    10921 cgcccgttgc aaagtaattt gcagcgacgc aagataacgc tgctgggcgc tgatttgatc
    10981 gttcaggctt tgacgtaatg ccagcgcatc tgccacttct ttaaaggcgt tctggatttt
    11041 ctgttcataa ttcaccaccg actgctgctg gcgaatttcg gcgatatcca gattggcctg
    11101 gttgcgtcca gcattaaaaa tggggatctc aattttagga ataaaattcc acatcccgct
    11161 gctggcatta aataatgacg acagatcgct gctggcggtt gatattccgc tggtcaggct
    11221 gatagacggg aaaaatgccg cacgcgcagc accaatattg gcattagccg ccattaacgc
    11281 gtgttcagct tccataatat cagggcgctg caataagatt tgcgacgaca agcccgccgg
    11341 taatttaacg ctttgcaggc tgtcgctgtt tactgtctgc gcttgcggca gcttgccgta
    11401 gcttcccaat aacagttgca atgcattatt tgcctgcgcc agttccccct gacgtttagc
    11461 gatgtcgctg cgggtacttt ctatcacccc gcgagcctgt tccagcgcca gaacattgct
    11521 gctaccggtc aacagttgtt tttcgacaaa cgcatatgac tgctgataat tacgcagcgt
    11581 ttcttcggct atttgcaatt gcgcatacgc cagttgctga ttgaaatagc tttgcgcgac
    11641 attagaaacc agcaaaatat gcaccgcgcg ctgagcttcc tcagtggcta aataattttg
    11701 gcgctcggct tcgctcatgt tctttaagcg cccgaaaaaa tcgagatcaa agctggcgtt
    11761 aaggccagtc gagaactccc gcgtcgtggc tgaatcgcct ttaagattgc cgctccagct
    11821 gccgctgccc tcgccattga gctgtgggta gcggtcggca tcggtcagac gatattgcgc
    11881 ccgcgcttcc tgcactttca gcgccgccat gcgcaaatcc cggttattca ccaacgcctc
    11941 gctaatcagc gtcttcacct gattatcaac aaaaaaggtg cgccagcccg cgttctggta
    12001 gttatctgct gcgttaacca ggccgttctg gctgagtgag aactgctgcg gcacgggcat
    12061 tgccggacgc tgataatccg gtgccagtga acaaccggtt agcgcaaggg ccacacaaaa
    12121 tggcagaagt ttacaaggag acataggctc ataatttctg gtgattttat gctgccaact
    12181 ttactcgcca ggctctgatt ttccggtgac aggaaaatga caaaattgtc attttgccaa
    12241 taagcgattg ccatctgatc ccgctactct agaattgccc gggcaacatg cggaggaaat
    12301 atgaaactgt tgattgtcga agatgaaaag aaaaccggag aatacttgac caaagggtta
    12361 accgaagccg gttttgtggt cgatttggcc gacaacgggc tgaatggcta ccatctggcg
    12421 atgaccggtg attatgatct gataatcctc gatattatgc tgccggacgt gaacggctgg
    12481 gatatcgtgc gcatgttgcg ttccgccaat aaggggatgc cgattctgtt gcttaccgcg
    12541 cttggcacca ttgaacatcg cgtcaagggg ctggagttgg gggcggatga ctatctggtg
    12601 aagccgttcg cttttgctga actgctggcg cgggtgcgca ctctactgcg gcgcggggcg
    12661 gcggtgatta tcgaaagtca gtttcaggtt gccgatctga tggtcgatct cgtcagccgc
    12721 aaagtcaccc gcagcggcac gcgcatcact ctgaccagta aagaatttac tctgctggag
    12781 tttttcctcc gccatcaggg cgaagtgctg ccccgctcgc ttatcgcctc gcaggtatgg
    12841 gacatgaatt ttgacagcga taccaacgcc attgatgtgg cggtgaagcg gctgcgcggc
    12901 aaaatcgaca acgactttga gccaaagttg attcagaccg tgcgcggcgt gggttacatg
    12961 ctagaggtgc cggatggtca gtaagccatt tcagcgcccg ttttcgctgg caacccgcct
    13021 gacctttttt atcagcctcg ccaccatcgc ggcgtttttc gcctttgcat ggatcatgat
    13081 ccactcggta aaagtgcatt ttgccgagca ggatattaat gatttaaagg agattagcgc
    13141 cacccttgaa cgggtactta atcaccctga cgaaacgcaa gcccgacgct taatgacgct
    13201 ggaagatatc gtcagtggtt attccaatgt gttgatctcc ctggcagata gccacggtaa
    13261 aacggtgtat cactcccccg gtgcgccgga tatccgcgag tttacgcgtg acgccatacc
    13321 cgataaagac gcgcagggcg gcgaggtgta tctcctttcc ggcccgacga tgatgatgcc
    13381 agggcacggt cacgggcata tggaacacag caactggcgg atgattaact tgccggttgg
    13441 cccgctggtt gacggcaaac cgatttatac gctctacatc gcgctttcga tcgattttca
    13501 tcttcattac atcaatgatt tgatgaataa acttattatg accgcatcgg taatcagcat
    13561 cctgatcgtc tttatcgtgc tgttggcggt acataaaggt cacgcgccga tccgcagcgt
    13621 cagccgtcaa atccagaata tcacctcgaa agatctcgac gttcgccttg acccgcagac
    13681 cgtgcccatt gagctggaac agctggtact gtcgttcaac catatgatcg agcgtattga
    13741 ggatgtcttt acccgccagt ccaatttctc agcggatatc gcccacgaaa ttcgcacacc
    13801 tattactaat ctcattacgc aaacggaaat cgccctcagc cagtcgcgca gccagaagga
    13861 gctggaagat gtgctctact ctaatctcga agagctgacg cgaatggcga aaatggtcag
    13921 cgatatgctg tttctcgctc aggccgataa caaccagcta atccccgaaa agaaaatgct
    13981 caacctggcg gatgaagtcg gcaaagtgtt cgattttttc gaggcgttag cagaagatcg
    14041 cggcgtggag ctgcggtttg ttggcgacga atgtcaggtt gcgggcgatc cgctgatgct
    14101 gcgtcgggcg ttaagcaatc tgctttctaa tgccctgcgt tatacgccaa ccgaagagac
    14161 aattgttgtg cgctgccaga cggtcgatca cctggtgcaa gttaccgtcg aaaaccccgg
    14221 tacgcccatt gcgcccgaac acttaccgcg attgtttgac cgtttctacc gcgttgatcc
    14281 ttcccgccag cgaaaaggcg aaggtagcgg cattggcctg gcgatagtga aatcgattgt
    14341 tgtcgctcat aaaggcacag ttgcagtaac gtcagatgtg cgggggacaa agtttgttat
    14401 catattaccc gcttaatcct tcagcaaacg gcaactttta taaccagtgt aaaaataaca
    14461 tgccgcaata gtaataccta ttaatgataa aaagctgtca caattcataa aaaaccttaa
    14521 tatacgccac cctaaacata accagcgtta atgtaaggtt tttgtgtgga ctggctcctt
    14581 gatgtttttg ctacctggct ctacggctta aaagtaatcg cgataacgtt agcggtcatt
    14641 atgttcatca gcgggctgga cgattttttt attgatgtcg tctactgggt acgccgcatt
    14701 aaacgcaagt tgagtgttta tcgccgctac ccgcgaatga gttaccgcga actgtataaa
    14761 ccagatgaaa aaccgttagc gattatggtt ccggcgtgga atgaaacggg cgtcatcggc
    14821 aatatggccg agctggcggc gaccacgctc gactacgaaa actatcatat ctttgttggc
    14881 acctacccca acgaccccga tactcagcgt gatgttgacg aagtgtgcgc tcgcttcccg
    14941 aacgtgcata aggtagtctg cgcgcgtcct ggccccacca gcaaagccga ctgtctgaac
    15001 aacgtgctgg acgccatcac ccaatttgaa cgtagcgcca atttcgcttt tgctggtttt
    15061 attctgcatg acgccgaaga tgtgatttca ccgatggaat tgcgtctgtt caactatctg
    15121 gtcgagcgta aagatctgat tcagatccca gtgtatccgt tcgaacgcga atggacgcac
    15181 ttcaccagca tgacctacat tgatgagttt tcagaactgc atggcaaaga tgttccggtg
    15241 cgtgaagccc tcgccggaca ggtgcccagc gcaggcgtcg gcacctgttt cagccgccgc
    15301 gccgtgaccg cactgttagc tgacggtgac ggtattgctt tcgacgtgca gagtctgact
    15361 gaagattacg acattggctt ccgcctgaaa gagaaaggta tgacggaaat ttttgtccgt
    15421 tttccggtgg tggacgaagc caaagaacgc gagcagcgta aatttttaca gcatgcacgg
    15481 acgtcaaaca tgatctgcgt gcgcgaatat ttccccgata ccttttcgac tgcggttcgg
    15541 caaaaatctc gctggatcat cggcattgtt ttccagggct ttaaaaccca caaatggacc
    15601 tccagcctga cgctgaacta ctttctctgg cgcgaccgca aaggggcaat cagtaacttt
    15661 gtcagcttcc tcgcgatgct ggtgatgatc cagcttttgc tgttgctggc gtatgaaagt
    15721 ttgtggcccg atgcctggca tttcctttct attttcagcg gcagcgcatg gttaatgact
    15781 ctgctgtggc taaactttgg cttgatgatt aaccgcatcg tgcagcgggt gattttcgtt
    15841 actggctact acggcctgac gcaggggctg ctatctgtcc tgcgtctttt ctggggcaac
    15901 ctgattaact tcatggccaa ctggcgcgcg ttaaaacagg tacttcaaca cggcgatcca
    15961 cgtcgcgtcg cgtgggataa aacaacgcat gacttcccca gcgtgactgg cgatacccgc
    16021 tcgttgcgtc cgttaggtca aatcctgctg gaaaatcagg tcatcactga agaacaactc
    16081 gatacagcac tgcgtaatcg cgtcgaaggt ctacgcctgg gcggttcaat gctgatgcag
    16141 gggctgatta gcgccgagca actggcacag gcgctggcag agcaaaacgg cgtggcgtgg
    16201 gaatccatcg atgcctggca gatcccttcc tcgctgattg ccgaaatgcc ggcctccgtg
    16261 gcgctgcatt atgcggtact gccgctgcgt ctggacaatg atgagttaat tgtcggcagt
    16321 gaagatggta ttgacccggt ttcgctggcg gccctgacgc gtaaagtcgg acgcaaagtg
    16381 cgttacgtca ttgttctgcg gggacaaatt gtcaccggat tacgccactg gtatgcacgc
    16441 cgacgcggtc acgatccgcg ggcaatgttg tacaatgcag ttcagcatca gtggctcacg
    16501 gaacagcagg ccggtgaaat ctggcggcaa tatgtgccgc atcagttcct gttcgcagaa
    16561 atactgacca cgctcggtca tattaatcgt tcagcaatta acgtgttgtt attgcgccat
    16621 gaacgcagtt ctctgccgct cggcaagttt ttggtcaccg aaggcgttat cagccaggaa
    16681 acgttggatc gcgtcctgac aattcaacgc gaattacaag tttcgatgca atcactatta
    16741 ctcaaagcag gtttaaacac tgaacaggtt gcacaactgg agtccgaaaa tgaaggagaa
    16801 taaccttaat cgcgtcatcg gatggtctgg tttactgctg acatctttat tgagtaccag
    16861 cgcactcgca gacaatatcg gcaccagcgc agaagagctg gggctgagcg attatcgcca
    16921 ttttgttatt tatccccgtc tcgataaggc gctgaaggca cagaaaaata acgacgaagc
    16981 aaccgccatc cgcgaatttg aatatataca ccagcaggtg ccggataata ttccgctgac
    17041 tttatacctt gcggaagcct atcgccattt tgctcatgat gaccgggcgc ggctgttgct
    17101 tgaggatcaa ctgaaacgtc accctggaga tgatcggctt gagcgcagtc tggcggctat
    17161 tcctgttgaa gtgaaaagcg ttacgactgt tgaagaactg cttgcccagc aaaaagcgtg
    17221 cgatgctgcg ccgaccctgc gttgtcgcag tgaagtcggg cagaatgccc tgcggctggc
    17281 acaattacct gtcgccagag cgcaactgaa cgatgcgacg tttgctgcat cgccggaagg
    17341 aaaaacgctg cgaaccgatc tgctgcaacg cgcaatctac ctgaaacaat ggtcccaggc
    17401 agatacgcta tacaatgaag cacgccagca gaacacatta agcgcggcag aacgccgtca
    17461 gtggtttgac gtgcttcttg ccgggcagtt ggacgatcgg atcctggcgc tgcaatcaca
    17521 ggggatcttc accgatcctc agtcatatat tacttacgcg accgcgctgg cttatcgtgg
    17581 cgaaaaagca cgcctccagc attatctcat tgaaaatacg ccgctattta ccacggacgc
    17641 acaagagaaa agttggctct atctgttatc taaatacagc ggcaaccccg ttcaggcgtt
    17701 ggcgaattat acggtacagt ttgccgacaa ccgccagtat gttgttggcg cgacgctacc
    17761 ggtgctgtta aaagaaggtc agtacggcgc agcgcaaaaa ctgctcgcca ccctccccgc
    17821 caatgaaatg cttgaggagc gttataccgt tagcgtagcg acccataaca aggctgaagc
    17881 tctgcgtctg gcacgattgc tgtatcagca agaaccggca aatcttaccc gcctggatca
    17941 actgacctgg caactgatgc agaacgaaca gtcacgcgaa gctgccgatt tattgctgca
    18001 acgctatcct ttccagggcg atgcgcgtgt cagccagact ttaatggcgc gactggcgtc
    18061 tctgctggaa agtcatcctt acctggcaac gccggcgaag gtggcgattt tatcgaaacc
    18121 cttaccgctg gcggagcaac gtcagtggca aagtcagttg ccgggtattg cagataattg
    18181 cccggcaata gttcgcttgc tgggcgatat gtcgccttcc tacgatgccg ccgcctggaa
    18241 ccgtctggca aagtgttatc gggacacgct acccggtgtg gcgttgtatg catggcttca
    18301 ggccgaacaa cgacaaccgg acgcctggca acatcgtgcg gtagcctatc aggcgtatca
    18361 ggttgaggac tacgccaccg cactggcggc ctggcagaaa atcagtcttc acgacatgag
    18421 caatgaggat ctgcttgctg ctgccaatac cgcccaggcg gcaggaaatg gtgcggctcg
    18481 cgatcgctgg cttcagcagg cagaacaacg tgggctggga aacaatgccc tctactggtg
    18541 gctgcatgcg caacgttaca ttcctggtca gccggaactc gcactgaacg atctcacgcg
    18601 ctcaatcaat attgcgcctt ctgccaacgc ttacgttgcg cgggcgacaa tttatcgcca
    18661 acgtcataat gtcccggcgg cggtgagtga tttgcgcgcc gcgctggaac tggaaccgaa
    18721 taatagcaac acccaggcag cgctcggtta cgccttgtgg gatagcggtg atatcgcaca
    18781 gtcgcgggaa atgctcgaac aggcgcataa agggctaccg gacgatccgg cacttatccg
    18841 acaactggcc tacgtgaacc agcgtctgga tgacatgcct gcgacgcagc actacgcccg
    18901 gctggtgatt gatgacattg ataatcaggc gctgataacc ccactgaccc cagagcaaaa
    18961 tcagcaacgc ttcaatttcc gccgtctgca tgaggaggtc ggtcgccgct ggacgttcag
    19021 cttcgattct tctatcggct tgcgttccgg cgcaatgagt accgctaaca ataatgtcgg
    19081 cggcgcagcg ccagggaaaa gctatcgtag ctacgggcaa ctggaagccg agtaccgcct
    19141 cggacgcaat atgctgctgg aaggcgacct gctctcagtt tatagccgcg tctttgccga
    19201 taccggtgaa aacggggtga tgatgccggt gaaaaatccg atgtccggca ccggtctgcg
    19261 ctggaagccg ctgcgcgatc agatcttttt cctcgccgtc gaacagcagt tgccgctgaa
    19321 cggacaaaat ggcgcatccg ataccatgct gcgcgccagc gcctcattct ttaatggcgg
    19381 caaatacagc gacgaatggc acccgaacgg ttcaggctgg tttgcccaaa acctgtacct
    19441 cgatgcggcg caatatatcc gccaggatat tcaggcgtgg acggcagatt atcgcgtaag
    19501 ctggcatcag aaggtggcga acggacagac tattgagcct tacgctcacg ttcaggacaa
    19561 cggttatcgt gataaaggca ctcagggcgc gcagcttggc ggtgtcgggg tccgctggaa
    19621 tatctggacc ggcgagacgc actacgacgc ctggccgcac aaagtcagtc tcggcgtcga
    19681 gtatcaacat acctttaagg cgattaatca acgtaacgga gagcgcaaca acgcgtttct
    19741 caccattgga gtgcactggt aaatgcgtaa gttcattttc gtattgctga cactgctttt
    19801 ggtcagccct ttttcctttg cgatgaaagg tattatctgg caaccacaaa accgtgatag
    19861 tcaggttacc gatacccagt ggcaggggct gatgagtcag ttacgtttgc aaggcttcga
    19921 taccctggtt ttgcaatgga cccgttacgg cgatgcattc acccagccag aacagcgcgc
    19981 gttattgttt aagcgtgcta cagctgcgca acaggctggc ctgaagctga ttgtcgggct
    20041 gaacgccgat ccggaatttt ttatgcacca gaaacagtcg tccgcagcgc tggaaagcta
    20101 tcttaatcgc ctgctggctg ccgatctcca gcaagccaga ttatggagcg ccgtgcctgg
    20161 cgtaacgccg gatggctggt acatcagcgc ggaaattgac gacctgaact ggcgcagcga
    20221 agccgcccgt cagcctttgc taacatggtt aaacaacgcg cagcggttga ttagcgatgt
    20281 ttcagcaaaa ccggtttata tcagtagttt tttcgccgga aacatgtcgc cagatggcta
    20341 tcgccaactg ctggaacaag ttaaagcaac cggtgttaat gtctgggtac aggatggcag
    20401 cggcgtggat aaactgaccg ctgaacagcg tgaacgttat ctacaggcca gcgccgattg
    20461 ccaaagttcc gccccagcaa gcggcattgt ttatgaactg tttgtcgctg gtaaaggtaa
    20521 aacgtttaca gcgaaaccga aaccggatgc agagatcgcc tcgctgttag cgaaacgttc
    20581 ctcttgcggt aaagacacgc tctatttctc tctgcgctat ttgcccgtcg cgcagagtat
    20641 ccttgagtat taatgattaa agggagcaac ttagtttgct cctttccttt tcactttcct
    20701 aatcaaaatc ctggcaagat gaattgttta gcagaatttc tgttttcatt ttattcgtct
    20761 gacgtgttcc aattatttag caaaaccgcg acaacaaagt gatggtgtaa tttgtaaaag
    20821 ggaacaaata aatggcgagg aaatggctcg ccctgcagga ttcgaacctg tagccacgac
    20881 ttagaaactc caggaacgac attttaaatc aacaaattac cgcgccatct ctgcgttcac
    20941 acgttccacc acctcaaaat atgtaaaacc ttgcaagcca ttgcgaggcc ttatgtgtct
    21001 cagttttgtt cctctttttt atactaaaaa acacagcaat tgaggataaa cctcatgcta
    21061 ttatcgctaa tatgcatcta aagg
//
LOCUS       DAZKHK010000093        20953 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKHK010000093 DAZKHK010000000
VERSION     DAZKHK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.093"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..500)
                     /locus_tag="ACHOS5_003475"
     CDS             complement(<1..500)
                     /locus_tag="ACHOS5_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9778237.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAR
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGR"
     gene            complement(497..2893)
                     /locus_tag="ACHOS5_003476"
     CDS             complement(497..2893)
                     /locus_tag="ACHOS5_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP9778238.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QANITSLLADIRKIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRTG
                     KRKEQQMLEEEDAEQRRSRSGLWGKISQWSGINNPGWEDYRKRDFDPENPEITLNNRR
                     EAIEYIEEIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            complement(3111..3545)
                     /locus_tag="ACHOS5_003477"
     CDS             complement(3111..3545)
                     /locus_tag="ACHOS5_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778239.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTALPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
     gene            complement(3767..4867)
                     /locus_tag="ACHOS5_003478"
     CDS             complement(3767..4867)
                     /locus_tag="ACHOS5_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hsdR"
                     /protein_id="HFP9778240.1"
                     /translation="MFDVDDTAPDTPAPRELSQDVQALINNGLDFLDKAREELEASKP
                     KFSVVSFWTAVEILLKVPLAHEHWSLVCSQKKPIKKQAYLAGDFQSVTYEETRERLKD
                     VLERPLDRETDSAFDKVRKHRNRVVHFYHPTFTEAEQRQILKEQADAWFALNRLLRDE
                     WKVIFGVKHNWKLAFGETRLIRGNKFYAEVRFKQVKPELEQLSEKNIQIGNCNECHQH
                     ATVTGTETTGNENRKLEVTRCKVCTSAVRQITLVCPDCEIPQLLPEGDSDFECEHCDY
                     TSDRYKLLDEELFHSVDEQLLSVFPAGCTNCMTPESVCKFGDGYLCTQCFIYYTELHV
                     CGCCGHLSDSVPELSHIRGCEFCDGDQRYFDD"
     gene            5266..5868
                     /locus_tag="ACHOS5_003479"
     CDS             5266..5868
                     /locus_tag="ACHOS5_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9778241.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVSALIKL
                     GNLRSKDYLFRSRVGTNQHISTRQYNRIFHGWVAKLGLEDSLYSTHSLRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
     gene            6096..6834
                     /locus_tag="ACHOS5_003480"
                     /pseudo
     CDS             6096..6834
                     /locus_tag="ACHOS5_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ETEC_3214 family protein"
     gene            complement(6952..7761)
                     /locus_tag="ACHOS5_003481"
     CDS             complement(6952..7761)
                     /locus_tag="ACHOS5_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778242.1"
                     /translation="MSLPVPFGAIVTVVKKCWSTLTQIMLIKTYQLSHERWAVRHRLG
                     ASWHSLGNYLEYSLRFAQLTDKEPRCSRIAFRSKEERLQQVELVFEAIGSGLRYQETL
                     CIHDVNNSPIILTLSNIPTLDVLILDDGFAFTVEQYQLKYCTIKLMTGESISIDNSPK
                     YSLMQNWCLNDTWKRRWGIIWNCNAIECARRRIAEYWKWGFCLPLVGRPPLYSPSRKG
                     IHLFEAKPLRWFMTRPWCLNIQFWTAIWSGLFILSDENVLQWRWKEPPQQD"
     gene            8244..10409
                     /locus_tag="ACHOS5_003482"
     CDS             8244..10409
                     /locus_tag="ACHOS5_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9778243.1"
                     /translation="MKTQITFAALLPALASFIPLHAHASSTSEDEMIVTGNTAADTTD
                     SAAGAGFKTNDIDVGPLGTKSWIETPYSSTTVTKEMIENQQAQSVSEMLKYSPSTQMQ
                     ARGGMDVGRPQSRGMQGSVVANSRLDGLNIVSTTAFPVEMLERMDVLNSLTGALYGPA
                     SPAGQFNFVAKRPTEETLRKVTLGYQSRSAFTGHADLGGHFDENKRFGYRVNLLDQEG
                     EGNVDDSTLRRKLVSVALDWNIQPGTQLQLDASHYEFIQKGYVGSFNYGPNVKLPSAP
                     NPKDKNLALSTAGNDLTTDTISTRLIHYFNDDWSMNAGVGWQQADRAMRSVSSKILNN
                     QGDISRSMKDSTAAGRFRVLSNTAGLNGHIDTGSIGHDLSLSTTGYVWSLYSAKGTGS
                     SYSWGTTNMYHPDAIDEQGDGKIRTGGPRYRSSVNTQQSVTLGDTVTFTPQWSAMFYL
                     SQSWLQTKNYDKHGNQTNQVDENGLSPNAALMYKITPNTMAYVSYADSLEQGGTAPTD
                     ESVKNAGQTLNPYRSKQYEVGLKSDIGEMNLGAALFRLERPFAYLDTDNVYKEQGNQV
                     NNGLELTAAGNVWQGLNIYSGVTFLDPKLKDTANASTSNKQVVGVPKVQANLLAEYSL
                     PSIPEWVYSANVHYTGKRAANDTNTSYASSYTTWDLGTRYTTKVSNVPTTFRVVVNNV
                     FDKHYWASIFPSGTDGDNGSPSAFIGGGREVRASVTFDF"
     gene            10420..11409
                     /locus_tag="ACHOS5_003483"
     CDS             10420..11409
                     /locus_tag="ACHOS5_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9778244.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            11428..12486
                     /locus_tag="ACHOS5_003484"
     CDS             11428..12486
                     /locus_tag="ACHOS5_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP9778245.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            12483..13250
                     /locus_tag="ACHOS5_003485"
     CDS             12483..13250
                     /locus_tag="ACHOS5_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9778246.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPALLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            13304..13561
                     /locus_tag="ACHOS5_003486"
     CDS             13304..13561
                     /locus_tag="ACHOS5_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778247.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(14086..14300)
                     /locus_tag="ACHOS5_003487"
                     /pseudo
     CDS             complement(14086..14300)
                     /locus_tag="ACHOS5_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388349.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15479..15631)
                     /locus_tag="ACHOS5_003488"
                     /pseudo
     CDS             complement(15479..15631)
                     /locus_tag="ACHOS5_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            15991..16191
                     /locus_tag="ACHOS5_003489"
     CDS             15991..16191
                     /locus_tag="ACHOS5_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778248.1"
                     /translation="MAQYDDDEHALQVSMQGRGYHHFRILPHLPETTGISYGYRQPLC
                     GMPGARTDPSDPSVLRSASDGR"
     gene            complement(16094..16702)
                     /locus_tag="ACHOS5_003490"
     CDS             complement(16094..16702)
                     /locus_tag="ACHOS5_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778249.1"
                     /translation="MKMKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFQN
                     LTPGWVSFNGEKPKIAIVPRSLHRLIYGSDKRATPPLDDDLVVNLCTSEHLLVHHPML
                     EGILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTENLK
                     RKSEKELGEWIIDRQKQNAALTDLMDQYVLLAYRTTVDGNRN"
     gene            complement(16790..16996)
                     /locus_tag="ACHOS5_003491"
     CDS             complement(16790..16996)
                     /locus_tag="ACHOS5_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9778250.1"
                     /translation="MTTPVSLMDDQMVDMTFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            complement(17380..18171)
                     /locus_tag="ACHOS5_003492"
     CDS             complement(17380..18171)
                     /locus_tag="ACHOS5_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778251.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVIDALLSATAPVL
                     NDINCYYQLAGKLQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTKRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRRIKDICEYQMQLSGEKAKYACFIYIERTIYNHD
                     NPSGGTPVKAAVGNAVILLAKDVIYDEYFFDLRQSFFVSVKDLMASGAKGIPETQKYP
                     DVYCWIPLFSINSGVVITPVYKIDPRKPVTVKKPDQITVVCNYRE"
     gene            19237..19386
                     /locus_tag="ACHOS5_003493"
     CDS             19237..19386
                     /locus_tag="ACHOS5_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP9778252.1"
                     /translation="MEKVISHARYKLTPEEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            19618..20217
                     /locus_tag="ACHOS5_003494"
     CDS             19618..20217
                     /locus_tag="ACHOS5_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9778253.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTGGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYALSYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLGSTLPETYSLV"
     gene            20589..>20953
                     /locus_tag="ACHOS5_003495"
     CDS             20589..>20953
                     /locus_tag="ACHOS5_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9778254.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASRHCRQC
                     HHDYYGERYCTHCHTGHFS"
BASE COUNT         5136 a         5140 c         5103 g         5574 t
ORIGIN      
        1 tatcgcccgg cgctccacca tgagaatatc tgctcaagat gtaactgcgc ccgctgaacc
       61 tggtcaacac ggtggagttt ttcttcaaga tgattcagtt tatggtgaac ctgatcggcc
      121 aggatgagta actgctcccg tgtatctgcg gaataatgcg ccagcctggc agtatcactg
      181 accagtgaac tgaccctgtc gtttacccgg gtaatcgcgt agtttgtggt ggcaagtgac
      241 gttgtcagct ccgtcagcca gcgcagggac gcctcaacgc cctgtgcaag cgaagcattg
      301 atggcattct gccgtgcagc gccttcgccg gtcagtccct cttttaaacg ccggaagaac
      361 gtgcgctctc tggccgtgcg gatatgctcc tgtgcaacat cgattccgtt tgcgaaatca
      421 accacaaatt tttcaggaag acaggtgagt acctgacata cccggttatc agcaatttgt
      481 tgaataaatg gtgaagtcac agcattgtct ccagctcttc cctcagcgcc ttactgtcag
      541 taatgagctc aggaaccctt tcatttaatg cctgaagtct cccggtaagc cgttcctgtg
      601 cctgctgatc gcgtttatga tcttcagagc tctgaatcag cgtgttacgc agttgttcaa
      661 ctttctccct gaatgcgcag aaaaaggcat cgatttcctg gcggacgggc tggttaatat
      721 cctgctcaat ggatgcctgc agctccccga aatatgcccg ggtcagtgac ataacttcct
      781 ctctgacctt gttgatattg atcacgctgc gactcacatc ctcgtcatag ttttcccagc
      841 ccaggtcact ggtgccaaac catcgacaaa cggttcccca tacgccactc tgccgacggc
      901 gacgggtgac ggtctccgtt ctttcctcca taagcgcact cagatttgtt tttaccgcga
      961 gccgggtctg taactgactg actgccggaa aactgatttt tacggtaaag ccctcatcct
     1021 ccaggcgggc attgatgcga ccggcaatgt tttctgtggc gtacagggcc gtgccacgaa
     1081 aacctgtttc aatcccgctg acgattttct ccagctcagg ccggaactgt gcttcagcct
     1141 cacggtgcag gctggtcaca gttgactcaa tttcctctat atattcaatt gcttccctgc
     1201 gattatttag tgttatctcg gggttttccg ggtcaaagtc tcgtttcctg taatcttccc
     1261 agcccgggtt gttgataccg gaccattgag aaatctttcc ccataatccg gaccgggacc
     1321 ttcgttgttc tgcgtcttct tcctccaaca tttgctgttc tttgcgttta ccagttctga
     1381 aatagtcatt cagctcctct tccagtgagg tgcagacccc cgtcagtaat tcccgggttc
     1441 tggcatcgat ctcctccatg gccattctga catcagcggt cacctgctcc tgacagtccg
     1501 cgattttccg gatatccgca agcaggctgg tgatattcgc ctgcagtgac tgaatgcttt
     1561 gctgcagccc ctgatggcgt aatgacagat attcgctgac attttctgca ttctgcatca
     1621 gttttgcggc agcagaatca accgccagtg ccgccgctct ggagtgtgaa ctcagtatga
     1681 cttccgttat cagttgatcg ataagtgagc cttcccacag gtctgtcgcc ccttcagttg
     1741 ccagttcgct gtctttacag acgtaatctt ttttcatgcg accgaaggct tccctgacaa
     1801 aatcatcgac ccaggcttca tcaacaggaa gggttccgtt tttccgtaac tcatgaagcg
     1861 cacggttagc caggtatgcc tggcgtgagg atccgggata aacgcgggag gcgggcagca
     1921 catcgctgtt cagcattgcc ggaactttct ggcgtacggc atctgcccca tcgccattgc
     1981 ggtctttctc atcaaattta ttgaccagta caaacaggcg gccggctgat acgtcagcaa
     2041 tggcattaag ctctttacgg acgtcttcat ccgcttttga gttcatctgg gtgtaatcca
     2101 tcacagccag aaccgcagag gctttctgca gttgatcccg catcatcact tccatctgcg
     2161 gttgtccggc ctcattaggg ccgggggtat ccagcagtgt gagtgttccc tggcatgcat
     2221 ccatccccac cagatggctg aattccacgt cgatcaccgg cagtttctgc acttctgcgt
     2281 attcatcaaa aggaaattca gtccccattg ccgcagcaag acgaaccaga tcgtttaacg
     2341 atgccagtcc ggtaaagatt tcctcctccc cgtggtattc atttttgagc cagccatcat
     2401 ccgtcagaat atccagcagt tcgcgggtat ccccggtttg ctgcagggtc tgtgcgacct
     2461 gctgtcctgc cggggtggcg agtttttcct gcagtgtgat taataaattg cggacaggct
     2521 gaatatgttc cagatggaga accggctcag tttttccggg aacgtggcgg atgagcgtcg
     2581 gtacagaggt catggggcgg ttacggttag gcagaatttc ctgccccaca atggcgttga
     2641 tggtggttga tttgcctgct ttcatcgtgc cgacaacagc cagcaccatc tccctgcggg
     2701 cggttttgat ttgttcgccc tccagctcct caattctttt cagtgctttc tgtttatcaa
     2761 aaagcagtct gttttcgttc ttactgtcag ataacacatc tggctcctca accatttgcc
     2821 ggagcagatt aatgttcagt tgcaaaagtc ggtcggcttc atcacaaagc agggcgatgt
     2881 tcttttcgtg cattatttaa ccttcctgag acgcattttc atgacaacca cggaggaggt
     2941 catttactac aaacgatcgt ttataacgat cgatatgata gtaacgatcg atgtattcta
     3001 ttaacttggg aagcacaacg caaggggaca ccccgggtat ggctgcgttt gcatagtgtt
     3061 ttcatggagt taaaaatctg taacaacagc ctggtacatc accaccgggt tcagaagaaa
     3121 atccagttcc ataccgcgcg ggcaacagag accaccgtgt cacgaactgc gcgcagggcc
     3181 gtacggacaa cagaggcaac acagacactc tccgccgtgt caaatatccg gtccaccgca
     3241 ccggtaaact gttcacgggt ctgagtgcgg acagactccg tgcgcagttc gtcctgcagg
     3301 cgtgtcatca gagggatggc ggcatgtccg ggaagcgctg tcatgagtgc actgaccggg
     3361 gtgacctccc cctgaaacag tgcattacag agtgaggatt taccggcccc gcttttaccc
     3421 ataatgccag gcacgggttc gtggctggtg agtttgcgca gatgttccag gatgtgacgg
     3481 gaaagtgagt aaggcaggga ggagagcggt ttttcaattg cctcaatggc atcagacgga
     3541 ttcatacatg tattcccggt gaaaaaagac aaaaccccgc gcaccggaaa ggtggcgggg
     3601 ggattcacgg agataatatc tgcctgaaat tatttggaat gcaggtatat gtggctgatt
     3661 tgtatcagct aatgagtaca ggtctgaatc taatctgaca gtccgctctg tgccaggagc
     3721 ggactgtcca acccatccct gaagagccta gattatgtat ggtaattcag tcatcaaaat
     3781 aacgttggtc accatcacaa aattcacatc ctcggatatg gctcagttca ggaacgctat
     3841 cgctcaggtg accgcaacaa ccacatacat gaagctcggt gtaatagatg aaacactgag
     3901 tgcaaagata gccgtctcca aatttacata cgctttcagg agtcatgcaa ttggtacatc
     3961 cagcaggaaa tacggagagt agctgttcat ccacactgtg gaacaactcc tcatccagca
     4021 gtttatacct atccgaagtg tagtcgcagt gttcgcattc aaaatcacta tctccctcag
     4081 gtagcaattg aggtatttcg cagtctggac aaactagggt aatttgcctt acagcactgg
     4141 tgcaaacctt acaccgcgta acttcaagct tacggttttc attaccggtc gtttcagtcc
     4201 ctgttaccgt cgcatgctga tggcactcat tgcagttacc gatttgaata ttcttttcag
     4261 agagctgctc gagttcaggc ttaacctgtt taaaccgcac ctcggcataa aatttgtttc
     4321 ccctaatcag acgagtttca ccgaaagcca gcttccaatt atgtttgaca ccgaagataa
     4381 ctttccattc atcccttaat aaccggttca gggcaaacca ggcgtcagct tgttctttga
     4441 gtatttgacg ttgttcagcc tcggtgaaag tggggtggta gaaatgaact acgcggttgc
     4501 ggtgctttct gaccttatca aaagcactgt ccgtttctct gtctaggggt ctttcaagta
     4561 cgtccttaag cctttcacgg gtttcttcgt aggtcaccga ctgaaaatca ccggctaggt
     4621 aagcctgctt ttttattggc tttttctggc tgcagaccaa cgaccagtgt tcgtgcgcca
     4681 gcggaacttt cagtaaaatc tcgaccgcag tccagaaact gacgacggag aacttcggct
     4741 tcgaggcctc caactcttca cgggctttat ccagaaaatc cagaccattg ttgatcagcg
     4801 cctgcacatc ctgactgagt tcacgggggg cgggagtatc gggtgcagta tcgtcaacat
     4861 cgaacatcat tatctccttt aatcagcttc caccacgcag ttgctacagc gcgttagttg
     4921 tatccagtgt aaactggact tggtagctgg cagccacatc gagcgcaaaa aaccgtaaat
     4981 taacttctgt agactcgtac ttttcgtgac ccatgcgtac ccgcaggatc ttcgggtgca
     5041 cgtgaccata aagcaggtgc ctaccaaagc tgtggcgatt cagatcccta aactgatggg
     5101 gtcccaaaaa gtatgccata gcaagtgtaa gtatccctaa aatctgcact attctctttt
     5161 tataaatcct ctgacatgtg aattagattg ccctaaagtg agcttccgcg cctcgctcat
     5221 aaggacaacc atactcaaat ctcccacatt gcaggagatt tgagtatgaa cacgtcaccg
     5281 tggaacaaag accgtatcat aggccaaaaa agaccacttc agatatctca tatctggggt
     5341 atccgaatcc gacttgaact ggaaggtaaa acacgcgatt tagctctgtt caacatggcc
     5401 ttggacagta agcttcgagg ctgtgatcta gtcaaactca aagtatctga tgttgcatat
     5461 ggtagctctg tttcaagcag agctacggtt ctacaacaga aaaccggcag ccctgtccaa
     5521 tttgagataa ccaaagggac aagagaagct gtttctgcat tgataaagct tggcaattta
     5581 cgcagtaaag actatttgtt ccgatctcgg gtcggtacta accaacacat atcaacccgg
     5641 caatacaacc gaatttttca tgggtgggta gcaaaacttg gtctcgaaga ttcgctttac
     5701 agtacacatt ccttgagaag aacaaaacct tacctgattt acaagaaaac taagaatctc
     5761 agggtgatcc aacttctgtt aggccataag aaactggaaa gcacagtccg ttatctgggc
     5821 attgaagtcg atgatgcgtt agagatctct gaatcgattg aagtctaagg ttgccagggc
     5881 tgcaacagca gtcctgtgcc agatgcggac gttgctctat tcatgtaaga ttaatagaat
     5941 gtctttcgat aaatttttga cttaatctta aataagaatg acttttttgc atgtcagcgt
     6001 tttactttgc attcagaaat aaatagaatt ttgaaaaaat aagagagaaa tcatgataca
     6061 ctccaaaaaa attaaaaaac aaaaagagag gaactatgtc acatccccct aaaaaactta
     6121 aaaaatcatt aattactgtt tttttgagtg gtattcttgc tgttcttgga tattttgttg
     6181 cattgaacgg agtgtttgat ttatatcaac atatcgcaga taattttttg caaaaaaaag
     6241 cagaggataa tcttcatgtg atttacaccg gagcgtcaat aaagtacatc gagtcaattt
     6301 ttggccctcc cgtgaaggaa gaacattctg aggatgggga gggttcatga gtatatttac
     6361 tctttcaaaa agttctatct gcaagtggtt tatgataata ataacacagt gatactctat
     6421 tccgtaacca gcaaggatga gaattttcat cctgaagtac catatctgag aaaaaccctt
     6481 ggacataagt tcaaagattt tggaagtgat attgattatt tacagtcgag ctattcttcg
     6541 aagttctatc aatatgaaga agggcattat ttaggaaatc ctggcaacta tagaaatttt
     6601 taccttgcct ataaccctgc tggagttgat tattttgagt tagatccact tccagatctc
     6661 tctaatgacg acaaatcgcc tccaaataaa aatgatcttg aaaaattcag aaataataat
     6721 gctccaaata catttggggt tggcgatatc cacggaggtc ctgaggggct tgagttaagc
     6781 tttggacttg ggattgatta ttacgatgcg agagatattc cagataaaga ttagtgatca
     6841 tgtatcatac catttttata tcgtttcaaa ttaggtagtc acaattagac tagttaatct
     6901 aaccagcact gaggtccgct cctcgctcag agcggacggc cttcgtgaaa gttagtcttg
     6961 ttgtggtggc tctttccagc gccattgcaa gacattctca tcgctgagaa taaacagtcc
     7021 tgaccaaatt gccgtccaaa actggatatt cagacaccat gggcgtgtca taaaccagcg
     7081 taacggtttt gcttcaaaca gatgaatacc ttttcgcgat ggtgaatata acggggggcg
     7141 tcccactaac ggcagacaaa agccccattt ccaatattca gcaatacgcc ttcgggcaca
     7201 ttcaattgca ttacagttcc agatgatacc ccaacgccgt ttccatgtat cattgagaca
     7261 ccagttttgc attagcgaat atttgggcga attgtcaata gatatacttt caccagtcat
     7321 taattttatc gtgcaatatt ttaactggta ctgttcaacc gtaaatgcga atccatcatc
     7381 tagaataagt acatcaagtg taggaatatt tgaaagcgtt aatattatcg gtgagttatt
     7441 tacatcatga atgcatagtg tttcttggta gcgtaagcca ctgccaatgg cttcaaaaac
     7501 aagttcaact tgttgcagtc gttcctcttt gcttctgaaa gcaatacgtg aacaacgagg
     7561 ttctttatct gtcaactgag caaatcgtag actgtactcc aaataattcc ctaaggaatg
     7621 ccagctcgcc cctagcctgt gtctcactgc ccagcgctca tgtgagagtt gataagtttt
     7681 tatcagcatt atctgggtaa gagtagacca gcatttcttc actactgtaa caattgcacc
     7741 aaaaggaaca ggaagtgaca ttactaaacc cctacaaata tcagataatt aggttttata
     7801 tcatttcagt cagtgtaatc aaatgataat caggattggg gctaatcttg aacgccacta
     7861 aagatcggca acaatgggga tccaatgttt cgttagtgat ttttcgcatt agcagataca
     7921 tgcttacttc cgctcctcgc tctcagcgga ccttcagctc agtgatatcg tccgctctgt
     7981 gccaaaggcg gacgctcaag tagatactgt cctgacagta tgcggtaatc agagttgtta
     8041 tatattactt gcgtccaaac cagctttctg acaacccggc cgctcctgct ttcaacaacg
     8101 cgccgccata cttttatgct gcaaagagcg gagacagaag gttagcgtaa tttgtaaata
     8161 acttacattc acgtgtgatc tggatcataa ttttgcctat gcaccgatcg taatataaat
     8221 atttacataa cgatcagtga ttcatgaaaa ctcaaataac tttcgctgcg cttttgccag
     8281 cattagcgtc tttcataccg cttcatgctc atgcctcgtc tacttctgaa gatgaaatga
     8341 ttgtcacggg caacaccgcc gccgacacca ccgattctgc cgccggtgcc ggtttcaaaa
     8401 cgaacgatat agatgtcggc ccgctgggaa cgaaatcctg gatcgaaaca ccatattcca
     8461 gcaccactgt aactaaagag atgattgaaa atcagcaggc gcaaagcgtc agcgagatgc
     8521 tgaaatactc tcccagtacg caaatgcagg cgcgcggtgg aatggatgtc gggcgtccgc
     8581 aaagtcgggg gatgcagggc agcgtggtgg ccaacagccg tctggacggg ctgaatatcg
     8641 tttcaacaac cgcgtttccg gtggaaatgc ttgagcgcat ggatgtgctt aacagtttga
     8701 ccggcgcgct gtacggcccg gcgagcccag cagggcagtt taatttcgtg gcgaagcgcc
     8761 caaccgaaga gacgctgcgt aaagtgacgc tgggctatca aagccgcagt gcgtttaccg
     8821 gccatgccga tctgggtggc cattttgatg aaaacaaacg gtttggctat cgcgtgaacc
     8881 tgcttgatca ggaaggggaa ggcaatgtgg atgacagcac gctgcgtcgc aaactcgttt
     8941 ccgttgcgct cgactggaat attcagccgg gcactcagct acagctcgac gccagccatt
     9001 acgaatttat ccagaaaggc tatgtcggta gctttaacta tgggccgaac gtcaaactgc
     9061 cgtctgcgcc gaatccgaag gacaaaaatc tggcgctcag cactgcgggc aacgacctca
     9121 ctaccgatac catcagcact cgcctgatcc actactttaa cgacgactgg tccatgaacg
     9181 ctggcgtggg ctggcagcag gctgaccgcg cgatgcgtag tgtttccagt aaaatactca
     9241 acaatcaggg cgatatctct cgttcgatga aggattccac cgctgccgga cgttttcgcg
     9301 tcctgagcaa caccgccggg ctgaatggtc atattgatac cggctctatc ggccacgatc
     9361 tgtcactttc taccacggga tatgtctggt cgctttatag tgccaaagga acaggttcca
     9421 gctatagctg gggtacaaca aatatgtatc acccggatgc gatagatgag cagggcgatg
     9481 gcaaaatccg caccggcggg ccgcgatacc gctccagcgt aaatactcag cagagcgtta
     9541 cgctcggcga tacggtgaca tttacgccgc agtggtcggc aatgttctat ctcagccaga
     9601 gctggctgca gactaaaaac tacgataagc acggtaatca aacgaaccag gttgatgaaa
     9661 atggtttaag tccgaacgcc gcgctgatgt ataaaattac ccctaacaca atggcctacg
     9721 ttagctatgc cgattcgctg gagcagggcg gtaccgcacc gacggatgag agcgtaaaaa
     9781 atgccggtca aacgctaaac ccgtatcgca gcaagcagta tgaagtgggg ctaaaatcgg
     9841 acatcggcga gatgaatcta ggcgccgcgc tgttccgact ggaacgtccg tttgcctatc
     9901 ttgatacgga taacgtgtat aaagagcagg gtaaccaggt taacaacggc cttgagttaa
     9961 ccgctgccgg gaatgtgtgg caggggctga atatttacag cggcgtgacc ttcctcgacc
    10021 cgaaactgaa agatacggcg aatgcctcaa ccagcaataa acaggttgtc ggcgtgccga
    10081 aagtgcaggc caatctgttg gcggaataca gtttgccgtc cataccggaa tgggtttaca
    10141 gcgctaacgt ccattatacg ggcaaacgcg cggcgaacga taccaacacc tcttacgcca
    10201 gcagctatac cacatgggat ttgggaacgc gttacaccac gaaagtgagc aacgtcccaa
    10261 ccactttccg cgtggtggta aacaacgtgt ttgataaaca ttactgggct tctatcttcc
    10321 catcgggtac cgatggcgat aacggttccc caagtgcgtt tatcggcggc ggccgcgaag
    10381 tgcgtgcatc cgtcaccttc gatttctgat ggataaatga tgaaactctc ccgtttattt
    10441 ccgctgctgg cgctgctgct ggcggccagc ctgcacgcag aaaatacgca caaggaggtg
    10501 cgtatcgcgt caccgtggcc ggcgcaaaat accattattg ccatgctggg gtacggcgat
    10561 aacattgtcg gaacctcgat gattgcaaaa aggatccccc tgtttcgcca gagcctgccg
    10621 cgcatagaga aagtcgcagc agttagcgta aacagcgggc atgaaatcaa tcccgaacag
    10681 attatcgcgc tgggggtcga tatgcttttt gtaccgcaga atatggtagt gcctcaacag
    10741 gcgttgctga aacaggctgg ggtgcaggtg ctggcgtttg aggcgaattc actgcgggcg
    10801 ttaacgcagc gcgtacagca aacggcagcg gtgctggggc ccgatgcgca gcaaaaagcg
    10861 ctcgcctatc aacgctactt cgatcgcaac gtggccctgg tcaccgggcg cctgaaagat
    10921 cttcctgcgt cacagcgcgt gtcgctctat cacagtatgg gcaatccgct gaccaccacc
    10981 ggtagaccct cgctaaacca ggactggatt gacctggccg gcggcaaaaa tatcgctgaa
    11041 aattggtttg gcgagcatca acaaaatcgt tcgggtgaag tcgcgctgga gaaaattgtc
    11101 actgccaatc cggcggttat catcgcgatg aacaagcgcg acgcggatgc cattctgagt
    11161 tctccgcagt gggcaagcgt ggacgcggtc attcatcatc gcgtttacgt taatccgaaa
    11221 ggaatgttct ggtggtgccg tgaaaccagc gaagaggcgc tgcaattttt gtggctggcg
    11281 aaaacgcttt atcctgcgcg atttgctgat gtggatatac gcaaagagac gcgtgagttt
    11341 tatcggcagt tctttggcct gacgttaagc gatgcgcaga tgagcgatgt gctcaatccg
    11401 ccgcgctaac ggaggctgtg aaaaaccatg actgtattgg aaagtacgct gtcggtcgag
    11461 aaccgctatc gccagctttt tcgccagcgt ctggtaatac tggcggttat ttttatcgct
    11521 atcattgctt cgctgctgct cgatttcacc ctcggcccct cagggctccc tttacattcg
    11581 ctgataaaga ctttgatgca tccgtccggg gcgactaacg gcatgcgggt tatcgtttgg
    11641 gacatccgtt tgccctatgc gctgatggcg ctcctggtag gaatggcgct gggtttggcc
    11701 ggggcagaga tgcaaactat tctgaataac tccctcgcca gcccgtttac gcttggcgtt
    11761 tcgtcggcgg cggcctttgg cgcagcgctg gcgattgttc tgggcatcgg tattcccggc
    11821 gttcccgaaa gcgggtttat accggcaaac gcttttcttt ttgcgctcct gtccgccctg
    11881 ctgctcgata gtctgacccg ctggacgcgc gtgccgacgt cgggcgttgt gctgtttggg
    11941 attgcgctgg tgtttacctt caatgcgctg gtgtccatta tgcagtttgt cgccgatgaa
    12001 gatactttgc agggcctggt gttctggacg atgggcagcc tggctcgcgc atcatgggag
    12061 aaactggcgg tgctggcggc ggcgatggcg attgttttac cctggtcgct gcgtcgtgcc
    12121 tggcagctta ccgcgctacg cctcggcgaa gagcgggcga tgagttttgg cattgatgtt
    12181 cgccgtttgc gcctgggttc cctgctgcgc atcagcctgc tggcggccct ttcggttgcg
    12241 tttgtggggc caattggctt tattggcctg gttgcaccgc atatttcccg cctgttgctg
    12301 ggagaagatc atcgctttta tttgcccgga agcatcctta tcggtgggct ggtgctatcg
    12361 ctggcctcgg tagcgtcgaa gaacatcatt cccggtgtca ttttaccggt aggaattgtt
    12421 acctcgctgg taggcgttcc gttctttctg agcattgtaa tgcgtcatcg agggagtatg
    12481 tcatgagcgg gcttacgatt aatgcgctgt gcgccggtta cggcaaacgg cggattattg
    12541 agcatctgtc gatttctacg ctgccgcgcg gcgaagtcac tgtactgctg gggcctaatg
    12601 gctgcggtaa atcaacgcta ctgcgcgccc tggccgggct aaatcgcgcc agtggcgaag
    12661 cctggctgaa tgaagagaat ctcttatcgc tgccgtttgc tcgtcgggct gaaaaagttg
    12721 tgttcctgcc gcagtccctg ccgcagggcg tgcatttaca ggtgctggag tcggtggttg
    12781 tcgcccagcg cgcctccggc gccgggcaaa atcaggcgca ggctatagcg ctgctcgaag
    12841 agctaggcat cgcacatctg gcaatgaact acctcgatag cctgtccggc ggtcagaagc
    12901 agctggtggg gcttgcacag tcgcttattc gccgccccgc attattattg ctggatgaac
    12961 cactgagcgc gctggatctc aattatcagt tccatgtgat ggatgttgta tcgcgcgaaa
    13021 cgcgaagacg caatatggtt acgctggtcg tcttacacga tatcaatatc gcactgcggc
    13081 atgccgccca ggtcatcatg ctgaaagagg ggaaacttat cgacagcggc gacccgcaaa
    13141 cggtgatcca tgcagagagc cttgcgcagg tatacggcgt acgcgggaga gttgaacgtt
    13201 gtgctcaggg aagatcgatg gtgatagtgg atggtgcaat cgaaaaatag tcggattaac
    13261 gtggttgctt cagcctcagg taaagatcaa taggaagtct gtaatgcaac atatagaccg
    13321 ccttaatgtc attaaagcac ttgtgcttct agaagatgag cagattgttc gttttaacat
    13381 cgctgcgaac gataacgcct cgcagatcca catgctcgtt gacggtctgg gcgtcgggct
    13441 gacacatgag cctgtcgctg ccggaatgat ttcgcaccgg acagcttgtc accgttctag
    13501 gcaacatgac agacctgcgc attcctattt ctattatcta tccgcctacc aagttgctta
    13561 atgtcaggct aaggcccttt aatgactgga tttcagaccg attttctgga caaccgagcg
    13621 ggtaaatcgc agtacactga cagctagaca ataaagctcc ggttcatgtt tgaaccatgt
    13681 gtagtgaatg tccgctgttc gctcacaacg gaccttcagc tcagccgttc cggccgcttc
    13741 atgccatgaa tgtacgcagc cagtcagaat attaaggtgc ggatttcagt tcacttgcca
    13801 gcccagtgat acaggacagt aatgaccaac aatttggtat tgatacaaca agctgcggcc
    13861 ccggttcgtg ggggagatga aacgaaggta gctgaagtta ctgaaatgaa gaattcagag
    13921 agactgaatg atatgcctca cctttagtga atagtgtaaa acgggagact ctcacccggg
    13981 attatccact catgctcaat gtataatttt cgtgcccctc acaggtaaga gccggataca
    14041 ttcgttgtgt atgctgcaga attggtaacc ggggccctgc agtctttaac tccgagtctt
    14101 gtgctgctca tttgccacaa aatcctggta cgccagctca atcatttcat tcaacagttt
    14161 ttttgggggg caccacgact tcgggcaata ctttcaggct gtttgtggat ttttccggac
    14221 agtttcagcg gaacggaacc atcagcacgt tcatcacgcc ttttttgttg ctcccgggca
    14281 cttttcagtt catccacaat atagctgctc ttctcgcatt tttgccgatt ttaaaaatgc
    14341 gaacaaaaca tccgtacatg atgtacagat taaactgcca gggactggtg tcagaaagcc
    14401 tccggttagc ctgctctttc cggaatgtgc cggttatgaa aaaccggaaa ttcagtctct
    14461 gttgtaagat gtaccctctg atactacgtt ttcgttttct ctgtgtattt aactcactgt
    14521 atttattgtc tttttaagcc tgactgatat tctccggtca tagtgtgctg gatagacatc
    14581 ccgtaacctt ctcattttta tataaaaata cctgcactgt cttcagcgca tctgtctcct
    14641 ttaagtaaaa aatgttttca cgtattgctg taaattcatt ttaaatcagt atgttatcct
    14701 gatattgctg tctcctggtt tgatgcgtaa tcgttctgac tttctggatg attctggtgc
    14761 ttccgggtgc attctcctct gcacatgcaa cagttcgtca tcgccacgtg cccaaaaagc
    14821 atataaaaac gaatcgaatc tgatcgtttt ttcaatcgtt tttcacagga atttcgtagg
    14881 gtgttctccg ctatagcctc catatttgct gtctttttct tcataaatcg tatgcaatat
    14941 aaccagtctg tcaagacatc ttccctgctg ttgaggtgac gatctcattt ctggtctttt
    15001 ccgggagaga gacggtcaga tgagcgagcc ggatttactg ccagaggtac tccccgcggc
    15061 agaaaatagt gctcctgttt ccgcaatccg ggatcggcaa cgtcccatgg ttaatgccac
    15121 ttgcagcgtc aggcactgca ggtttacgtc gtgatctgat ttcttctgca cttatcccga
    15181 tcacggaagt tccccctgcg cagacatcgg ttcctgccga catttcacaa taccgccagg
    15241 ctgtcatgca gtaacacccg caatgcggtc tgcggtttta agcgcactct cctgttttca
    15301 gtgcaccgat aagcgttata gaaagtctgg ggtgagttac tgaatgtctg ttaagtggtg
    15361 ccgtgggtgg agactgctta cttttccaca gcaactgcag tcattcagga tgctcaggag
    15421 agaatatgtc aacgccacga gccgggccat tttcatgcca gccagtatat tttctacact
    15481 gatgcagtct gattttcacc attccatcct cactgattgt cgtgttgttt tgggtcatca
    15541 ccacagcttt ccgtttctct tttaatcggt aacgctctcc tttgatattc agcgtggttg
    15601 agtgatgcag cagtcaatcc aggagcgctg ttgtccggca accaccatat caacttcttc
    15661 ccggcgtgcc ggcgctggta tccaggccgg gtttcgaagc gaagcgttcc tgacggactc
    15721 attgacatcc gggccggatg tagtcatgca gcatggaacg tccgcccgta tacactgacg
    15781 gcgtccggcc gtgtacagag atcggtaaaa ttcgttacta caactgttcc tgaatatctg
    15841 acgaagaaac attcagccat aacatccggc cgtgtacatc tcttttgcgg tgcagggagt
    15901 gctgtcgcca tgtacttatt gtcaccaggg tgaggcatgt accgcagcat gccagctcag
    15961 caatgacagg atgcccttaa gacatccgtc atggctcagt atgatgacga cgaacacgct
    16021 ctgcaggtga gcatgcaggg ccgggggtat catcatttcc gtattttgcc gcaccttccg
    16081 gaaacaaccg gcatcagtta cggttaccgt caaccgttgt gcggtatgcc aggagcacgt
    16141 actgatccat cagatccgtc agtgctgcgt tctgcttctg acggtcgatg atccattccc
    16201 ccagctcttt ttctgatttc cgtttcaggt tctccgtcca gtcgtcaagg ctgtttccgg
    16261 tcattaaatc aagccagcaa agccagacca gtttgaggcg caactccgtg ccaccaaact
    16321 gacggaagag gctgatcagt ttatttgcca gagaacgctg cctcagacgt tcacattcag
    16381 acagcagaat cccttccagc atcggatgat gaaccagcag atgttcgctg gtgcataagt
    16441 tcaccaccag atcatcatcc aggggcggcg tggcccgttt gtcagagcca taaatcagcc
    16501 ggtgcagact ccggggtacg atggcaatct tcggtttttc accattgaat gacacccacc
    16561 cgggcgtcag attctggaag tagtggatat tcggatgaca cacgccccag gatacggcgt
    16621 gccgggtgaa gccggtcagc catttctggt agctttcgtg gagcagctcg taacgggtct
    16681 gatactgtgg tttcattttc atattcctca cgggggataa caggcagcag agtgccgccg
    16741 gcaggccgga gattgcgtct gctgtgggcg aagggccagt ggttaaaaat tacggacggg
    16801 actgtgcaat acgcgcctgc agccaggctt ccacttcact tttcagccag cgggagctgc
    16861 ggccgagttt gatgggtgcc ggaaaggccc catccttgat gagcttgtaa aaccacttat
    16921 cggtcaggcc ggtcagctga gtgataaacg tcatatcgac catctggtca tccatcagcg
    16981 aaactggggt ggtcataaaa atctcctccg gatgttgtta atctcagccg gcgttgatga
    17041 tgaaaacaac gcaccacctt caccggcata acaaccgggg agaaacgtaa aaaatcagta
    17101 ggggaatatt cctgccagga gagaaactgt tgtgtacagg caatacaggt tgtctgtttt
    17161 gtcagtgaag ccggtataaa cggaagatga ttttgtgttg ccggaatatg ggatggcgtt
    17221 ttgccgtgac gggatcagta ttttcagtga tgattattac tgtgcagggg agtaccggag
    17281 ataaaccacg ccctgtacct gccagagcgg aggtttgtat ttactgaacg gaagataaag
    17341 cgaggtaaac actgttgcgg tgagcggaga agactgagat tattctctgt aattacaaac
    17401 aacagtgatc tgatccggtt tttttaccgt tacgggtttc cggggatcaa ttttatagac
    17461 cggggtaatg accacgccgg aatttatgga gaaaagcggg atccagcaat aaacgtccgg
    17521 atatttctgt gtctccggaa taccttttgc gccagaggcc atcagatctt taacgctcac
    17581 gaaaaatgac tgccgcagat caaaaaagta ctcgtcatat atcacgtctt ttgccagcag
    17641 gatgactgca ttaccaacag ccgcttttac aggtgtccca ccggatggat tatcgtgatt
    17701 gtatattgtt ctttcaatat aaataaaaca ggcatatttt gccttctccc cggacagttg
    17761 catctggtat tcgcaaatgt ctttgatacg ccggctttca acccgtccaa tcatcttatc
    17821 ccggcgatag tgtcttttat gtttcagcgc gtacagaaca ccatttgaac tgtcaaactc
    17881 caggattctt ttggtgaaaa gtttgttttc ccagatctcc agacaatact tctctgcact
    17941 gacgacattg cgtttgtgag gaagatcccg ttgatacact ccatttaata agcgtgactg
    18001 cagtttgcct gcaagttgat aataacagtt aatatcgtta aggacaggtg ctgtggcgct
    18061 gagtaatgcg tcgattacct tagagcgcga aagtttgtgg gtatgacagt actcatccac
    18121 aattcgcagc gtctcatcac tggtcatatt gaaagttata cgtgttgtca tgatgaaatt
    18181 ttctgttaat catactggtt gatatgcata acatttcata aaaaatactt ttaattcata
    18241 taattgattt tttgactggt tttatcctcg tgtggtggaa agtagtgtta ctgccactgt
    18301 agtcagcata actctatcac acagaaccac cgaaaatgaa gcattgctca ggggggaagc
    18361 tgccactcgt acgagttttc tgagcaatga taaccagtat ttttcgtttt gggcgatttg
    18421 tttatcgtta cagctaaaaa cagttcatgt accattaaaa aataataaag gttaattagt
    18481 attatttata gaagtatgtt tatgtaattc taccgtgtaa atgaactctg tcattgtacc
    18541 ttgtaattct atcctgtaat tctacctcgt aaatacacct tgtaaccaag tcctgcagaa
    18601 gtctgtctca tcgaacaata ttcctgtgca gcgatattct ctgtagtagt acttgtaact
    18661 ctgcccttaa ctctctcttc agtaatgtag tcccgccaca cctgaatata aatctgtaat
    18721 gttcacacac tgactgtaac gtgctttgtt aagtaatgtc tgctattttt ttctgttatt
    18781 tttcctgttg ttttctgtat tttgttgtat ggtgtaataa ctattctgaa cccgtcatca
    18841 cctttatagc taagagtatg acttcgccag tgttgatact gtggataacg gtgattaagg
    18901 aattactgtt aatgatctgt tcctgcagct cacctgatga gttttggtag taatacgtaa
    18961 aagagtatgc ctcatgtcat taaaaagtct tccggagtgt tgtttaaccc tgtacctcta
    19021 ttactcatgt agtacgttgc ggaaagctac gttaattgta atatctggtt atggcgttat
    19081 gccttcagta ttaaaaacat cttttaccgg agaaacaggg aagtggaaag gctttcggga
    19141 taacgtcagg ccattatgac gatattacgt taatcacagt acaagataac gagagggtaa
    19201 tggaatttta aaccagagta gttccactgt tgttcactgg aaaaagtgat ttcccacgcg
    19261 cgttataagc ttactccaga ggagatggaa gccttcaact ctgcggtcga tcaccggctg
    19321 gcagaactga ccatgaacaa actttacgat cgtgttcccg cttcagtctg gaaatatgtt
    19381 acctgataaa tgtttctgat gaagtacagt cgtcacaatg acatcacttc agatattatc
    19441 tgcgctgtga ttgactgtca cgtatgccgt tctctgtatg gttctgatac ccgggtgtgg
    19501 tcccactttc tctgtggcgc ccggggagtt gtgaacctgc acgtcttcgt gtgataacgt
    19561 tttcttcaaa aattacgcca gcgcttcttc ctgtgttaca gaacacgagg aagaaacatg
    19621 aagacttaca acaccaatcc cggttacgaa atggatcccc agctcctgac acactttaac
    19681 cagcatctgg attcattatt cggggtatat tcgaaactgt taccgttcag aatggatttt
    19741 gcttaccgaa aaaacaccct cagctacagg tgtgcctgca ggtacgccat gtgtgcggag
    19801 atactgcgac tgattaacga agtgggagag aaactggtcg ggtatgcctg ggtaatggaa
    19861 tatacggaac gtaaagggct tcacattcat tttgtgggct atctgaacgg tcagagccac
    19921 aggagttcgt atctggtatc cagattgatg ggagatatct ggaggcgggt taccgggggg
    19981 gatggttact accactggtg ccggtttaat aaaaattatc cggtaaacat taaccacgtc
    20041 attcactatt cagatcacaa agcggtgaat gccttacgct atgccctcag ctacctggcc
    20101 aaacgagaac agaaagaatg tggcatcatc ctgaaatgca gcgggcttcc tgaaaaaagt
    20161 aaccggggtc gaccgcgact gggcagcacc ctgccggaga cttattctct ggtgtaagac
    20221 cctgaacgac aactacgggc tggcgctaat tgttacggtc ggttccggag gccacttcag
    20281 ggactctgcg cattttttac gacatggcta ttccgtatga accatacgga gatttaacca
    20341 tggcacattc tttaccattc cttccggcaa ccgatacagc gcatcccccg atgtccatcc
    20401 ctgcctactg acagtttttt acgccggtgg gccattgtgt gacaaccaca gtgctgtaac
    20461 agcaaccagc ctgctttacc caaactccag tccagatttt aactgaaccg ccattcaccc
    20521 cctgaccaca ctgcccgtac cgggacagct gttgcctgcg tgtcgtctgg cggctgagga
    20581 atatgacgat gtacgcaaaa tcctttattg catttgatgg caacgggcgt ctgacgggtg
    20641 cccgtaccgc acagactgca ccttacgatc gctacacttg ccacctttgc ggcagtgcgc
    20701 tcagatacca cccgcaatac gacactgagc gtccctggtt tgaacacact gacgacgggc
    20761 tgacggagca cggtcagcag tgcccttatg tcagaccgga acgcagggag atacaactga
    20821 ttaagcgtct gcagcaattc gtaccggatg ccttacccgt ggtgcgtaaa gccagccggc
    20881 actgcagaca atgtcatcac gattattatg gggagcggta ctgcacgcac tgccataccg
    20941 ggcacttcag tga
//
LOCUS       DAZKHK010000094        20944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKHK010000094 DAZKHK010000000
VERSION     DAZKHK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.094"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..644
                     /gene="fimA"
                     /locus_tag="ACHOS5_003496"
     CDS             81..644
                     /gene="fimA"
                     /locus_tag="ACHOS5_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9778255.1"
                     /translation="MKLKHVGIIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLVLDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            1006..1716
                     /locus_tag="ACHOS5_003497"
     CDS             1006..1716
                     /locus_tag="ACHOS5_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9778256.1"
                     /translation="MQTTRTPYSVSFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTHLAMAPEEAPA
                     MLRFRRSGSKFTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAVTGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1758..4409
                     /locus_tag="ACHOS5_003498"
     CDS             1758..4409
                     /locus_tag="ACHOS5_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9778257.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTFALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMASRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSADKHVPDNSACTPLQDRLADASSEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSTPKFVQASLMHGLKGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADDTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTSEQTLFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATGYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            4423..4953
                     /gene="ydeS"
                     /locus_tag="ACHOS5_003499"
     CDS             4423..4953
                     /gene="ydeS"
                     /locus_tag="ACHOS5_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778258.1"
                     /translation="MKYSNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            4966..5469
                     /gene="ydeR"
                     /locus_tag="ACHOS5_003500"
     CDS             4966..5469
                     /gene="ydeR"
                     /locus_tag="ACHOS5_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778259.1"
                     /translation="MKRLHKRFLLATFCTLFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPIETSTVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5529..6443
                     /gene="ydeQ"
                     /locus_tag="ACHOS5_003501"
     CDS             5529..6443
                     /gene="ydeQ"
                     /locus_tag="ACHOS5_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778260.1"
                     /translation="MGKTFSIKVLLGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKTLATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGAVQSVIGVTFIYE"
     gene            6782..9061
                     /gene="ydeP"
                     /locus_tag="ACHOS5_003502"
     CDS             6782..9061
                     /gene="ydeP"
                     /locus_tag="ACHOS5_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP9778261.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLCERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            9309..9506
                     /gene="safA"
                     /locus_tag="ACHOS5_003503"
     CDS             9309..9506
                     /gene="safA"
                     /locus_tag="ACHOS5_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP9778262.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            9582..10343
                     /gene="ydeO"
                     /locus_tag="ACHOS5_003504"
     CDS             9582..10343
                     /gene="ydeO"
                     /locus_tag="ACHOS5_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP9778263.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNKMSALAI"
     gene            10745..12427
                     /gene="ydeN"
                     /locus_tag="ACHOS5_003505"
     CDS             10745..12427
                     /gene="ydeN"
                     /locus_tag="ACHOS5_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9778264.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYDSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSEAK"
     gene            12479..13636
                     /gene="ydeM"
                     /locus_tag="ACHOS5_003506"
     CDS             12479..13636
                     /gene="ydeM"
                     /locus_tag="ACHOS5_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9778265.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(13602..13727)
                     /locus_tag="ACHOS5_003507"
     CDS             complement(13602..13727)
                     /locus_tag="ACHOS5_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778266.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            13927..15612
                     /gene="yddA"
                     /locus_tag="ACHOS5_003508"
     CDS             13927..15612
                     /gene="yddA"
                     /locus_tag="ACHOS5_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9778267.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSENFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYMSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            15650..18022
                     /gene="yddB"
                     /locus_tag="ACHOS5_003509"
     CDS             15650..18022
                     /gene="yddB"
                     /locus_tag="ACHOS5_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9778268.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFTVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            18079..20862
                     /gene="pqqL"
                     /locus_tag="ACHOS5_003510"
     CDS             18079..20862
                     /gene="pqqL"
                     /locus_tag="ACHOS5_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9778269.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVSDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVR
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSSMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLPQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYNSRTPVNLTTRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTTVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         6083 a         4556 c         4610 g         5695 t
ORIGIN      
        1 cttgttttat aagcaataac agaataactt aattgttaac tctctttatt cagctactta
       61 aatataaatt ttggagaatt atgaaactca aacatgtcgg tattattgtc gtttctgtgt
      121 tggcgatgtc gtctgctgcg gtaagcgcag ccgagggtga tgaatcagta acgaccactg
      181 ttaatggcgg tgtaattcat tttaaaggtg aagtggtaaa tgccgcttgt gcgattgatt
      241 cagaatcaat gaaccaaacg gttgagctgg gtcaggttcg ttcttctcgc ctggctaaag
      301 cgggtgacct cagctccgcc gttggcttca atatcaagct gaatgattgt gataccaatg
      361 tttccagtaa tgcagctgtt gcattcctgg gtactactgt caccagtaat gacgatacat
      421 tagcgctgca gagttcagcg gcaggctctg cccaaaatgt cggtatccaa attttggacc
      481 gtacagggga ggtattagta cttgatggtg ctacttttag cgctaaaact gacttgattg
      541 atggcacgaa tatactgccg ttccaggctc gttatattgc tctcgggcag tcagtagctg
      601 gtactgcaaa cgctgatgcg accttcaaag ttcaatatct ataattcact cttaaggtga
      661 ctatgcgtca tctgaaattg aaacagggtg tgtgtggcat ggctacgcac acccgagaaa
      721 ttcaccaatg ttcatctcat tattaatgaa aaatggtttg tattttggag cggtatgaaa
      781 aatcacataa tatctaaacg atcaataaac aacagtagtt aaacttgctg ttatttaaaa
      841 tatttacttg gtagcaaggt tttcctatca aaagagaacc aacatttatt acaaacgcat
      901 taagtctgga aacccggcga ctcgccaatg tattagcaat atttaccgat tgtctgatgt
      961 aacggtttcc ccgcttatcc tcttaacaca tggagaatgc atcagatgca aactacgcgt
     1021 accccctact ctgtttcctt tatggcgaca gtgttacttt tattgctctt tgcttgccat
     1081 agcaccattg ccaacgcagc tgtcgcactt ggtgcgacgc gggtaattta tccggcaaac
     1141 cagaaacagg ttttattgcc tgtcacgaat aatgatcctg ccagtgtcta tctcattcag
     1201 tcatggatag aaaatgcggg tgaccagaaa gatacccagt ttgtcattac cccaccgctt
     1261 ttttccatgc aaggtaaaaa agagaatacg cttcggataa tcaacgccac taatcatcag
     1321 ttaccaggcg accgtgaaag cctgttctgg gttaatgtta aagctatccc tgctatggaa
     1381 aaagaccaga agaatgagaa tacattacag ctggcaatta ttagccgcat aaaaatgttt
     1441 tatcgcccca cgcatctggc aatggcacct gaagaagcgc ctgcaatgct tcggtttcgt
     1501 cgcagcggta gcaaattcac tcttattaat ccaacgccct acttcattac cgtaacaaat
     1561 atgaaagcag gaaatagcaa tctcccaaac accatggtgc cgccaaaagg tgaagtatcg
     1621 gtggatatcc cgcatgctgt aaccggggat atcagttttc agacaatcaa tgattacggt
     1681 gcattaactc cgcgtattaa ggcaaccatg cagtaagcca atgacctaat taataataaa
     1741 ctcaatcggg attaaaaatg acagcctttc gtgccgcatt taaggcatat cgtatgcatc
     1801 aggtgttaat actgcctcgc tttgcacggc ttacctttgc ccttggctta gcaactgcgg
     1861 tttttcccgt tgacgcagag tattatttta atccacggtt tttaagcaat gaccttgctg
     1921 aatctgtcga tttatctgcc tttactaagg gacgcgaggc cccacctggt acatatcggg
     1981 tcgacattta tttgaacgac gaatttatgg ctagtcggga tattactttt attgcagatg
     2041 ataataacgc agatctcatc ccatgcctga gtacagacct tcttgtcagt cttggcataa
     2101 aaaaatcagc attattagac aacaaggaac attcggcaga taaacatgtg ccggacaaca
     2161 gcgcatgcac accacttcag gatcgtttgg ctgacgcatc atctgagttt gatgttggtc
     2221 agcaacatct gtcactctcc gttccgcaaa tatatgtcgg cagaatggct cgcggctatg
     2281 tttctcctga tctgtgggaa gaaggaataa atgccgggct actaaactat agttttaatg
     2341 gtaattctat taataaccgt agtaaccata atgcaggaaa atccaactat gcatatttga
     2401 atttacagag tggcatcaac attggtagtt ggcgactgcg cgataactca acgtggagtt
     2461 ataacagtgg gagtagcaat tcatctgaca gcaataaatg gcagcatatc aatacgtcgg
     2521 ctgaacgtga cattattccg ttacgctcac ggttaacagt gggtgatagt tataccgatg
     2581 gcgatatttt tgacagtgtg aactttcgtg gccttaaaat aaattcaaca gaagcgatgt
     2641 tgcccgatag ccaacatggt ttcgctccgg tgattcatgg tattgctcgt ggcaccgcac
     2701 aagtgagtgt aaaacagaat ggatacgatg tttatcagac tactgtccca cccggccctt
     2761 ttactattga tgatatcaac tctgcggcca atggtggtga tctgcaagta accataaaag
     2821 aggcagacgg cagtattcag acattatatg ttccttattc gtctgttccg gttctccaac
     2881 gtgctggata tacgcgttat gcacttgcca tgggggaata tcgtagtgga aataacctgc
     2941 aaagcacccc caagttcgta caagcaagct tgatgcatgg tctgaaagga aactggacac
     3001 cttatggcgg aatgcaaatt gcagaagatt atcaggcctt caaccttggt attggtaaag
     3061 atttaggact ttttggtgcc ttttctttcg atattacgca ggccaatacg acacttgcag
     3121 atgacacccg tcacagcgga caatcggtta aatccgtcta cagcaaatcc ttctaccaga
     3181 cgggaaccaa tatccaggtc gcaggatatc gctattctac gcaaggtttt tataacttat
     3241 ccgacagtgc ctacagtcga atgagtggtt acaccgtcaa gcctcctacc ggagacacca
     3301 gtgagcagac actatttatt gattatttta atctgttcta cagtaagcgt ggtcaggaac
     3361 aaataagcat ctctcagcag cttggaaatt atggtacgac atttttcagt gccagtcgcc
     3421 aaagttactg gaacacgtca cgcagcgacc agcaaatatc atttggatta aatgtgccgt
     3481 ttggtgatat tacgacttcg ctgaattaca gctattccaa taatatatgg caaaacgatc
     3541 gggatcattt actcgctttt acgcttaatg ttccctttag tcattggatg cgtacagaca
     3601 gtcagtcggc atttcgtaat tcaaacgcca gttacagtat gtcaaacgat ttgaaaggcg
     3661 gcatgaccaa cctatcaggg gtttatggca ctctgctgcc ggataataac ctgaattata
     3721 gtgttcaggt cggtaacacc cacgggggta atacatcgtc tggcaccagt ggttacagtt
     3781 ctcttaatta tcgtggagct tacggtaata cgaatgtcgg ttacagtcgg agtggtgaca
     3841 gcagccagat ttattacgga atgagtggtg gcattattgc tcatgctgat ggcatcacct
     3901 ttggacagcc gctgggcgac acaatggttc tggttaaggc tcctggcgct gataatgtca
     3961 aaatagagaa ccagaccgga attcataccg actggcgtgg ctatgccata ttaccatttg
     4021 cgacaggata tagagaaaat cgtgtcgctc ttaacgcgaa ttcccttgca gataatgttg
     4081 aactggatga gaccgtggtc actgtcatcc caactcacgg tgctattgcc agagcaacat
     4141 ttaatgcaca aatcggcggg aaagtattaa tgacgttgaa gtacggtaat aagagcgttc
     4201 cattcggtgc aattgtcact cacggagaga ataaaaatgg cagcattgtc gcggaaaacg
     4261 gtcaggttta tctgactgga cttccacagt cagggaaatt acaggtttca tggggcaaag
     4321 ataaaaactc aaactgtatt gtcgagtaca agcttcctga agtttctcct ggtaccttac
     4381 tgaaccagca gacagcaatc tgtcgctaag gaacaataga ttatgaaata cagtaacatt
     4441 attttcctcg gtttatgtct gggcttaacc acctattctg ctttatctgc agatagcgtt
     4501 attaaaatta gcgggcgcgt cctcgattat ggctgcacag tctcatcgga ttcgcttaat
     4561 tttaccgtag atctccaaaa aaacagtgcc agacaatttc caacgaccgg tagcacaagt
     4621 ccagccgtcc cttttcagat tacattaagt gaatgcagca aagggacaac gggagttcgg
     4681 gttgcattta acggtattga ggatgcagaa aataatactc tgttgaaact agatgaagga
     4741 agcaatacgg cctccggttt gggtatagaa atactggacg gaaatatgcg tccggtgaaa
     4801 ttgaatgacc ttcatgccgg gatgcagtgg atcccattgg taccagaaca gaacaatatt
     4861 ttgccttact ccgctcgttt gaagtcaact cagaagtccg tcaatccagg actggtgagc
     4921 gcttcggcaa cctttaccct tgaatttcaa taatcagagt cagatatgaa aagacttcac
     4981 aagaggttcc tgttagctac gttttgcaca ttattcacag caactctcca ggccgccgat
     5041 gtcactatca ctgttaatgg tcgggtagtc gctaaaccat gcactattca aaccaaagaa
     5101 gctaacgtta atctcgggga tctttataca cgcaatctgc aacaacctgg ttctgcatct
     5161 ggctggcaca atattacttt gtcattaacc gattgtccga ttgaaacaag tacagtgacg
     5221 gcaatcgtga caggttcaac tgacaatacg ggttattaca aaaatgaagg tactgccgaa
     5281 aatattcaga tagagctaag ggatgaccag gatgcgacgt taaaaaatgg cgatagcaaa
     5341 acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc aagagctatc
     5401 acggtgaatg gaaacgcaag ccagggtacg atcgaggcgc taatcaatgt gatctatacc
     5461 tggcaataac atcaatttga ttttatttcc ctccttttta aatcaatttt ttaaccttag
     5521 gatatatcat gggtaaaaca ttcagtataa aggtcctgct cggtatttat cttctattaa
     5581 tggcaggcaa agtttttgcc ttcagttgta atgtcgatgg cgggtcaagt attggagcag
     5641 gtacaacctc tgtttatgtt aatctcgatc ctgtaataca accgggccag aatctggttg
     5701 tagacttgtc tcagcatata agttgctgga atgattacgg cggctggtac gacactgatc
     5761 atataaacct ggtacaagga tcagctttcg caggctccct acagtcatat aaaggatcac
     5821 tgtactggaa taatgtgacc taccctttcc cgttgacgac aaataccaac gtattggata
     5881 ttggtgataa gacaccgatg ccattaccgt taaaactcta tattacccca gtcggagcgg
     5941 ctggtggagt tgtcataaaa gcaggggaag ttattgcccg tattcatatg tataaaattg
     6001 ccacgttagg aagcggaaat ccgcgtaatt ttacgtggaa tattatttca aataacagtg
     6061 ttgttatgcc aacaggcggt tgcaccgttg atagtcgtaa tgtcaccgtg aatctcccgg
     6121 actttcctgg aagcgcagaa ataccccttg gtgtatattg ctctagcgaa caaaagctct
     6181 cattttatct ttcgggtgcc accactgaca gtgcccgaca agtgtttgct aacacagcac
     6241 cagatgcaac aaaagcatcg ggcgtgggtg tctctctgat gcgtaatgga aaaacactgg
     6301 caacaggaga aaatgtctcc ctggggacag taaataaaag caaagtacct ctgggtttaa
     6361 gcgcaaccta tggacaaaca ggaaacaagg ttgcagccgg agccgtccag tcagtcattg
     6421 gtgtcacttt tatctatgaa taattgtgat tttgacatta gtctatgcgc tccattcatg
     6481 aaaattgcgc ggataattat tttgtgaagg ctattatcct acacttgtaa gaaaatccgc
     6541 gcacaattga agccagagtc ttatttttta tattgagaat tatgttaatt taaaccctat
     6601 gagagatgat gcttaactta tcgtatttat actactctat tatttaacga taatagagta
     6661 aaaatagtaa gagagtagtc ccctactctt atatattcat gttgacgata aaccctttgt
     6721 atatactttg catcatcgct attacaaatc ctaataattc atttccacac aggataagca
     6781 gatgaagaaa aaaattgaat cctaccaggg agctgccggt ggttggggtg ctgttaaatc
     6841 cgtagcgaat gcagtacgta agcagatgga tatacgccag gatgttattg ccatgtttga
     6901 catgaataag ccagagggct ttgactgtcc gggatgtgca tggccagatc ctaagcacag
     6961 tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc gcctgggaag tcacggataa
     7021 gcaggtaaat gcctctttct ttgctgagaa tacggttcaa tcattactta cctggggaga
     7081 tcacgagctt gaggctgcgg gacgtctcac tcagcctttg aaatatgatg acgtcagcga
     7141 ctgttacaag ccattaagct ggcaacaagc tttcgacgaa attggcgctc gccttcaaag
     7201 ctatagtgat cccaatcagg ttgaattcta tacttcgggg cgcacttcca atgaagctgc
     7261 ctttctttat cagctttttg cccgtgaata cgggagcaat aactttcccg actgctccaa
     7321 catgtgccat gaaccgacca gtgtgggatt ggcagcgagt atcggtgtgg gtaaagggac
     7381 cgtgttgctg gaagactttg agaaatgcga tttagtcatt tgcattgggc ataaccctgg
     7441 tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta gtgaaacggg gagcgaaaat
     7501 gatcgctatc aaccctctac aggaacgtgg cctggagcga tttaccgcac cgcaaaaccc
     7561 gtttgaaatg ctgacgaact ctgagactca gttggccagt gcctactata acgtgcgcat
     7621 tggtggcgat atggcattgc tcaaggggat gatgcgcctg ttaattgagc gcgatgatgc
     7681 tgcaagcgcc gcaggtcggc cctcattgct ggatgacgaa tttattcaaa cgcataccgt
     7741 cggctttgac gagctacgcc gtgacgtgct caattccgag tggaaagata tcgaacgtat
     7801 ttctggacta agtcagacac aaatcgccga acttgctgat gcatatgccg ctgccgaacg
     7861 aactattatc tgttacggaa tggggatcac tcagcacgaa catggtaccc agaacgtaca
     7921 gcaactggtc aatctgctgt tgatgaaagg taacattggc aagcctggtg cgggtatctg
     7981 cccactacga ggtcactcta atgtacaggg cgaccgaacc gtcggtatca ccgagaaacc
     8041 gtctgcagag tttctggatc gcctgtgtga gcgctatggc ttcaccccac ctcatgcacc
     8101 tggacatgct gcaatcgcca gcatgcaagc tatatgtacg gggcaagctc gagcattgat
     8161 ctgcatgggg ggcaactttg cactggcaat gccagatcgg gaagcgagcg ctgtaccgtt
     8221 aacgcaatta gatctggcgg tacacgtagc cactaagctt aaccgctctc atctgctgac
     8281 cgcacggcat agctatattc tgccggttct gggacgtagc gagattgaca tgcaaaaaag
     8341 cggtgcgcag gcggtaaccg ttgaagattc aatgtcgatg attcatgcct cgcgtggtgt
     8401 gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgca gtggtcgcgg gaatcgcgca
     8461 agcagcacta ccccagagcg tggtagcctg ggagtatctg gtggaagatt atgatcgcat
     8521 tcgcaatgac attgaagctg tgctgccaga gtttgccgac tataaccagc gcatccgtca
     8581 tcctggtggt tttcacctga taaacgcagc agctgaaagg cgctggatga cgtcgtcagg
     8641 taaggctaat ttcattacca gtaaagggct gttagaagat ccctcttcag cgtttaacag
     8701 taagctggtc atggcgacag tacgcagtca cgatcagtac aacacgacga tttatggtat
     8761 ggatgatcgc tatcgagggg tattcggtca acgagatgtg gtctttatga gtgctaaaca
     8821 agctaaaatt tgccgtgtaa aaaacggcga aagagttaat cttattgcac tcacgccaga
     8881 cggtaaacgt agctcacgcc gcatggatag attaaaagtg gtcatttacc ctatggctga
     8941 ccgctcactg gtgacctatt ttccagaatc gaatcacatg ctaacacttg ataaccacga
     9001 tccattaagt ggcattcctg gctataaaag tattccggtt gaattagaac catcaaatta
     9061 attcgcctcc tcatttcttc tgctgtcatc cgcacagcag aagaattcct cattgactat
     9121 tatcgcgcaa tttgctcaca tggattaaat taaaccacac actataagat ataaacttct
     9181 gcctacagct gtaagaaact ccgctcagta ctgaagtacc cgtcctattt cctctattct
     9241 ccagcctgtt atattaagca tactgattaa cgatgcttaa cgtcattcgt ttaataaaca
     9301 catttgaaat gcatgcgacc atagtgaaaa ataaaatcac gcaaagagac aactataaag
     9361 ggatcatgtc tgtaattgtg gttgtcttat tactgaccct tacattgata gccatttttt
     9421 cggcaattga tcaactgggt atttcagaaa tgggtcgcat tgcaagggat cttacacatt
     9481 tcattatcaa tagtttgcaa gactgaaaaa taaacagcaa attataaata tgaaatgtta
     9541 aaaaagtatc gataaaaact ttattgtttt aaggagataa aatgtcgctc gtttgttctg
     9601 ttatatttat tcatcatgcc ttcaacgcta acattttaga taaagattac gccttctctg
     9661 acggcgagat cctgatggta gataacgctg ttcgtacgca ttttgaacct tatgagcggc
     9721 attttaaaga aatcggattt aatgaaaata ccattaaaaa atatctacaa tgcactaaca
     9781 tccagacagt gacggtgcct gttcctgcga agtttttacg tgcttcaaat gtaccgactg
     9841 gattgcttaa tgaaatgatt gcttatctca actcggaaga acgcaatcat cataattttt
     9901 cagaactttt gcttttttct tgcctgtcta tttttgccgc atgcaaaggt ttcattacac
     9961 tattaactaa cggtgtgcta tccgtttccg ggaaagtgag aaatattgtc aacatgaagc
    10021 tggcgcaccc atggaagctg aaagatattt gtgactgcct gtacatcagt gaaagcctgt
    10081 tgaagaaaaa actaaagcaa gagcaaacga cattctcaca gattctttta gatgccagaa
    10141 tgcagcacgc aaaaaatttg atacgcgtag aaggttcagt caataaaatt gccgaacaat
    10201 gtggttatgc gagtacatct tattttattt atgcgttccg caaacatttc ggcaacagtc
    10261 caaagagagt ttctaaggag taccgttgtc aaaggcacac gggtatgaac acggataaca
    10321 agatgagtgc tttagctatt tgattatttg ctaacgagta gtcaaccaca cacgctgcgt
    10381 aagaattaaa tggggcagcc attccctgcc ccgcgttgtt tttaggtgat atatttcttg
    10441 aaataaataa gtgacagcca tcacatattt atgcatttgc ataacccgtt gcatgattat
    10501 ttatgatctc aattcggtat tttgtcggta aaattcaata atttcttaaa tatcaataaa
    10561 ttagttgttt atcaacgaga aattacttag tagaacagaa agtaatgtca acgcgttatg
    10621 gactgttatt tccctttttt tagctaaatc tgctatctct ttatgtgact aacttcactt
    10681 acattcactt atttctcttc gtaaaattac tttggaatta agtacaataa gaagaggaac
    10741 atttatgaag tctgcattaa agaaaagtgt cgtaagtacc tcgatatctt tgatactggc
    10801 atctggtatg gctgcatttg ctgctcatgc ggcagatgat gtgaagctga aagcaaccaa
    10861 aacaaacgtt gcgttctcag actttacgcc gactgaatac agtaccaaag gaaagccaaa
    10921 tattatcgta ctgaccatgg atgatcttgg ttatggacaa cttccttttg ataagggatc
    10981 ttttgaccca aaaacaatgg aaaatcgtga agttgtcgat acctacaaaa tagggataga
    11041 taaagccatt gaagctgcgc aaaaatcaac gccgacgctc ctttcattaa tggatgaagg
    11101 cgtacgtttt actaacggct atgtggcgca cggtgtttcc ggcccctccc gcgccgcaat
    11161 aatgaccggt cgagcgcccg cccgctttgg tgtctattcc aatactgatg ctcaggatgg
    11221 tattccgcta acagaaactt tcttgcctga attattccag aatcatggtt attacactgc
    11281 agcagtaggt aaatggcact tatcaaaaat cagtaatgtg ccggtgccgg aagataaaca
    11341 aacgcgtgac tatcatgaca acttcactac cttttctgcg gaagaatggc aaccgcaaaa
    11401 ccgtggcttt gattacttta tgggattcca tgctgcagga acggcatatt acgactcccc
    11461 ttcactgttc aaaaaccgtg aacgcgtccc cgcaaaaggt tatatcagcg atcagttaac
    11521 cgatgaggca attggcgttg ttgatcgtgc caaaacactt gaccagcctt ttatgcttta
    11581 cctggcttat aatgctccgc acctgccaaa tgataatcct gctccggagc aatatcagaa
    11641 gcaatttaat accggtagtc aaacagcaga taattactac gcttccgttt attctgttga
    11701 tcagggtgta aaacgcattc tcgaacaact gaagaaaaac ggacagtatg acaatacaat
    11761 tattctcttt acctccgata atggtgcagt tatcgatggt cctctgccgc tgaacggggc
    11821 gcaaaaaggc tataaaagtc agacctatcc tggcggtact cacaccccaa tgtttatgtg
    11881 gtggaaagga aaacttcaac ccggtaatta tgacaagctg atttccgcaa tggatttcta
    11941 cccaacagct cttgatgcag ccgatatcag cattccaaaa gaccttaagc tggatggcgt
    12001 ttccttgctg ccctggttgc aaaataagaa acaaggcgag ccacataaaa atctgacctg
    12061 gataacatct tattctcact ggtttgatga ggaaaatatt ccattctggg ataattacca
    12121 caaatttgtc cgccatcagt cagacgatta cccgcataac cccaacaccg aggacttaag
    12181 ccaattctct tatacggtga gaaataacga ttattcgctt gtctatacag tagaaaataa
    12241 tcagttaggt ctatacaaac tgacggatct acagcaaaaa gataaccttg ccgccgccaa
    12301 tccgcaggtc gttaaagaga tgcaaggcgt ggtaagagag tttatcgaca acagccagcc
    12361 accgcttagc gaggtaaatc aggagaagtt taacaatatc aagaaagcat taagcgaagc
    12421 gaaataacta aaccttcatg cggcggattt ttccgccgcc ttattgagcg agatagcgat
    12481 gcacgttaca gccaagccct ccagttttca atgtaatctc aaatgtgatt actgttttta
    12541 ccttgaaaaa gagtcgcagt ttactcatga aaaatggatg gatgacagca ctctgaaaga
    12601 gttcatcaaa caatatatcg cagcgtctgg caatcaggtc tattttacct ggcaaggcgg
    12661 tgaacccact ctggctggcc tggatttttt ccgtaaagtt attcactatc aacaacgcta
    12721 tgcaggccaa aaacgaattt ttaatgcatt acaaacgaat ggcattttat tgaataatga
    12781 atggtgtgcc tttctcaaag aacatgagtt tctggttggt atctcgatcg atggacccca
    12841 agagttacat gaccgctaca gacgcagtaa ttcaggtaac ggtacttttg caaaagtgat
    12901 agcagccatc gagcgtctga aatcatatca aatagagttt aatacgttaa ctgtcattaa
    12961 taacattaat gtccattacc ctcttgaggt ttatcatttt ttaaaatcta tcggcagtaa
    13021 acatatgcaa tttatcgaat tgctcgaaac cgggacgccg aatattgatt tcagtggtca
    13081 tagtgagaac acattccgta tcattgattt ttctgtgcct cccacggctt atggcaagtt
    13141 tatgtcaacc atttttatgc aatgggttaa aaacgatgtg ggtgaaattt tcatccgtca
    13201 gtttgaaagt tttgtcagcc gttttttggg gaatgggcat accagttgta ttttccagga
    13261 gtcctgcaag gataatctgg ttgttgaaag taatggagac atttacgaat gcgaccattt
    13321 tgtctatcca cagtacaaaa ttggaaacat taataaatcg gaactcaaaa cgatgaacag
    13381 tgtacaactg acagcgcaaa aaaaacggat ttcagcgaaa tgtcagcaat gtgtatataa
    13441 acctatctgc aatggtggtt gccctaagca tcgtattact aaagtaaaca atgagactgt
    13501 ttcctatttt tgcgaaggtt ataaaatcct tttttcaacc atggtacctt atatgaacgc
    13561 catggtagag ttagctaaga acagagtacc gctttaccac attatggatg ttgcaaaaca
    13621 aatggagaat aattaataac gctccccctt agctccattc ttagcctcct tcttatgacc
    13681 ttaacaaggc acccgacata aattcctgtc gggtgtttcg tcttcatcat cttcccttgc
    13741 gaaaatcctg gcttacgagg tatgcgtcag ggcattagca atgctcttga ttatcaatag
    13801 cataaccata accactgaaa atgaaatatt tctttatatt tcatattgtt aaatgatact
    13861 taaataaatt tcatcagtat atcattgcca tagataatca ttggatggca caatcaattt
    13921 tcgctaatga taaccattcc cattacgttt tgtatgttga tagcaaagta tctctgtctg
    13981 ctgaagccct tttggctgcg taagaataat aaaacttcag tgttgttaat cattattatc
    14041 ctcgccatga tccttggcgt ggttaaaatt caggtctggt taaacgactg gaataatgat
    14101 tttttcaatg cgctaagcca gaaagaaact gataagcttt ggcagcttgt cctttggttc
    14161 cctgccctgc tggggatctt tgtattaata tccgtaaaca aaacatggtt aattaaacta
    14221 ctgactattc gctggcgcga atggttaaca gattactacc ttaatcgctg gtttgcagat
    14281 aaaaactact acttcacgca gatctacggc gagcataaaa atacagataa ccctgaccaa
    14341 cgtatcgctg aagacattct cttactgatc agcaaaacgc tcagtctttc atttggcttc
    14401 atccagtcac ttagcatgct gatcaccttt accgttattc tttggcaaag cgcgggtacg
    14461 ctctcattca ctgttggtgg aactgaatgg aatatccagg ggtatatggt ctataccgtc
    14521 gtgctcattg tgatcggtgg aactttattt acccataagg ttggtaaacg tattcgtccg
    14581 cttaatgtgg aaaaacaacg tagcgaagcg acatttcgaa ctaatcttgt gcagcataat
    14641 aagcaggcag aactgattgc gctaagcaat gctgagtctc tacaacgcca ggagttgagc
    14701 gaaaattttc ataccattaa agagaactgg catcgtttaa tgaatcgtca gcggtggctt
    14761 gattactggc agaatattta ttcgcgctcg cttagcgttc ttccctactt tctgttattg
    14821 ccgcagttta ttagcggaca gatcaatctg ggcggactga tgaaatcgcg ccaggcattt
    14881 atgctggtat cgaacaattt aagctggttt atttataaat atgatgaact tgctgaactg
    14941 gctgcggtta tcgatcgttt gtatgagttc catcaactca ccgaacagcg ccctacgaat
    15001 aagcctaaaa attgccaaca tgcggtacaa gtggctgacg cgagtattcg tactcctgat
    15061 aataagatca tattagagaa cctgaacttt catgtttcgc caggcaaatg gctattactg
    15121 aaaggttact ctggtgcggg aaaaaccaca ctgcttaaaa cattatccca ctgctggccg
    15181 tggtttaaag gtgatatttc ttctcctgct gacagttggt atatgtcaca aacaccgtta
    15241 ataaaaagcg gcttactgaa agagattatt tgtaaagcac ttcccctgcc cgtagacgat
    15301 aaatcgttga gcgaagtact gcatcaggtt ggtctgggga aattggctgc gcgtattcat
    15361 gaccacgatc gctggggaga tattctttcc agcggcgaaa aacaacgtat cgccctggca
    15421 cgattaattt tacgacgtcc gaaatggata tttcttgacg aaactacctc tcatcttgag
    15481 gaacaagagg ctatccgctt actgcgttta gtgcgtgaaa aactacccac aagcggcgtc
    15541 attatggtta cacatcaacc cggtgtctgg aacctggctg atgatatttg tgacattagc
    15601 gcggttttat aaaaacaata aaagccctgc tgtacggaga taaaaataaa tgaagcgagt
    15661 tcttattcct ggcgtcattt tatgtggcgc tgatgtggcg caggccgtcg atgacaaaaa
    15721 catgtacatg catgtttttg aagagatgac ggtctatgct cctgtccctg tacccgtaaa
    15781 cggcaacacg cattacacca gtgaaagcat cgagcgttta ccgaccggga atggcaatat
    15841 cagcgatctg ctgcgaacaa accctgcggt gcgcatggat tcaacgcaaa gtacctcgtt
    15901 gaaccaggga gatattcgcc cggaaaaaat ctctattcac ggtgcgtcgc cctaccagaa
    15961 tgcctatttg attgacggta ttagtgcaac gaataacctg aacccagcga atgagtccga
    16021 tgccagtagt gcaaccaata ttagcgggat gtcacagggg tattatcttg atgtcagctt
    16081 actggacaat gtgacgcttt atgacagttt tgtgccggtt gaattcggtc gcttcaatgg
    16141 cggggtaatt gatgcaaaga tcaaacgctt caacgctgat gatagcagcg tgaaactggg
    16201 ttatcgcact acgcgttctg actggttaac atcgcatatc gatgagaata acaagagcgc
    16261 atttaatcaa ggttcttcag gaagtactta ttactctcca gattttaaaa agaactttta
    16321 taccttgtcg tttaatcagg aactcgctga taactttggc gttaccgccg gtttatcgcg
    16381 ccgccagtct gatatcaccc gcgcggatta tgtttcgaat gacggcattg tcgccggccg
    16441 ggcacagtat aaaaacgtta tcgatactgc attgagcaaa tttacctggt ttgccagcga
    16501 ccgctttacc cacgatttaa ccttaaaata tactggctcc agccgtgatt ataataccag
    16561 caccttcccg gagtctgatc gcgaaatggg taataaatcc tatggtctgg catgggatat
    16621 ggatacccag ctcgcatggg ccaaactgcg taccaccgtt ggttgggatc atattagtga
    16681 ttatacccgt cacgatcatg acatctggta caccgaactt tcatgtacat atggtgatat
    16741 tacagggcgt tgcacccgtg gcggattagg acacatttcc caggctgtag ataattacac
    16801 cttcaaaaca cgcctggact ggcaaaaatt caccgtgggt aatgtttctc atcaacccta
    16861 cttcggcgcg gaatacatct attccgatgc atggactgaa cgccataacc agtctgaatc
    16921 ctatgtgatt aatgctgccg gaaagaaaac caaccatacc atttaccata aaggtaaagg
    16981 cagcctggga attgacaact acacgctgta tatggctgat cgcattagct ggcgtaatgt
    17041 gtcgttaatg cccggcgtgc ggtatgacta tgataactat ctgtcaaacc acaatatctc
    17101 cccgcgtttt atgacggaat gggatatttt tgctgatcaa acctcaatga ttaccgcagg
    17161 ttataaccgt tactatggcg ggaatattct tgatatggga ttacgtgata ttcgcaatag
    17221 ctggacggaa tcggtatcag gcaataaaac cctgacgcgt tatcaggatt tgaaaacgcc
    17281 ttataacgat gaactggcaa tgggattgca gcaaaaaatc ggtaagaacg ttattgcgcg
    17341 cgcaaactat gtttaccgtg aagcgcatga tcaaatcagc aaaagcagtc gtaccgacag
    17401 cgcgactaaa accaccatta ctgaatataa caacgacggc aaaaccaaaa cgcattcgtt
    17461 caacctcagt tttgaactgg ccgaacccct gcatatcagc caggtagata ttaacccgca
    17521 aattgtcttt agctatatca agagcaaggg caacttgtcg ttaaacaatg gttatgagga
    17581 gagcaatacc ggtgataacc aggtggttta taacggtaat ctggtctctt acgatagcgt
    17641 tccagtggcg gattttaata acccattaaa gatctcctta aacatggatt tcacgcatca
    17701 accgagcggg ttggtgtggg cgaatacgct gacctggcaa gaagcgcgta aagctcgcat
    17761 tatcctgggt aagacgaatg cgcaatacat cagcgaatat tcagattaca agcagtatgt
    17821 tgacgaaaaa ctggatagca gcctgacctg ggacacccgc ttgtcctgga cgccacaatt
    17881 tctgaaacaa caaaacctga cgatcagtgc cgatattctc aatgtactgg atagcaaaac
    17941 cgctgttgat acaacgaata ccggtgtggc gacctacgcc agtggccgta ctttctggct
    18001 tgatgtcagc atgaaatttt aatagttcgg attttactta accgggtaaa ttcgcccggt
    18061 tttcgcatgg agataattat gagaaacctc tgtttcttac tgacgttagt ggcaactctg
    18121 ttgctccccg ggcgactgat tgccgccgcc ttaccgcagg atgaaaagtt aattaccggg
    18181 caactggaca atggcttgcg atatatgatt tatccgcatg ctcaaccaaa ggatcaggta
    18241 aatttatggc tgcaaattca taccggttca ttgcaggaag aggacaatga gcgcggcgtg
    18301 gctcattttg tagaacatat gatgtttaac ggcacaaaaa catggccggg taataaagtc
    18361 atcgaaacat ttgagtcaat gggcctgcgt tttggtcgcg atgttaatgc ctataccagc
    18421 tatgacgaaa cagtgtatca ggtgagtttg ccgactacgc agaaacaaaa tctgcaacaa
    18481 gtgatggcaa tcttcagtga atggagtaat gccgcaacct ttgaaaaact cgaagtagac
    18541 gctgaacgcg gcgtaattac tgaggaatgg cgtgcccatc aggatgcaaa atggcgcacc
    18601 tctcaggcgc gccgcccttt cctgctggca aatacccgta atttagaccg tgaacctatc
    18661 ggcctgatgg ataccgtcgc cacggtcaca ccggcacaat tgcgccaatt ttatcaacgc
    18721 tggtatcaac caaataatat gacctttatt gtggtcggcg atatcgacag taaagaagcg
    18781 ctggcgctga taaaggataa tttaagtaag cttccggcta acaaagcagc tgaaaatcgc
    18841 gtctggccga caaaagccga aaaccacctg cgctttaata tcatcaatga taaagaaaac
    18901 cgggtgaacg gcatcgcact ctattatcgc ctgccaatgg tgcaagtgag cgatgagcaa
    18961 agctttatcg aacaagctga atggagcatg ttagttcagc tgttcaatca acgtctgcag
    19021 gaacgtatac agtcgggcga gttgaagact atttctggcg gcactgcgcg cagcgttaga
    19081 attgcacccg attatcagtc gctgtttttc cgtgtaaatg cacgagacga taatatgcag
    19141 gatgctgcga atgcattaat ggcagagttg gcaactattg atcagcatgg tttttctgct
    19201 gaagaactcg atgatgtcaa atctacccgc ctcacctggc tgaaaaatgc ggttgatcag
    19261 caagctgaac gtgatttacg tatgctgacc agtcgcctgg catccagctc attaaataat
    19321 acgccgttct tgtcgccgga agagacatat caactttcga aacgtctgtg gcagcaaatt
    19381 accgtgcaaa gtctggcgga aaaatggcag cagttaagaa agaaccagga cgcattttgg
    19441 gagcaaatgg taaacaatga agttgccgcc aaaaaagcat tgtctcctgc ggctatcctg
    19501 gcgctggaaa aagagtacgc caacaaaaag ctggcggctt acatcttccc aggcagaaat
    19561 ttatcgttaa cagtagacgc tgacccacag gcggaaatta gcagcaaaga aacgctggct
    19621 gagaatctga catcattaac actttccaat ggtgccaggg ttattctggc aaaatccgcg
    19681 ggtgaagagc aaaagctaca aattactgcc gtatctaata aaggcgattt aagtttccct
    19741 gcgcagcaaa aatcacttat cgcgctggca aataaagcag taagcggaag tggcgttggt
    19801 gaactctcct cttccagcct gaaacgctgg agtgcggaaa actcggtaac catgagcagt
    19861 aaagtcagta gcatgaatac gttgctctcc gttagtgcgc ggactaataa ccctgaacct
    19921 ggtttccagt tgattaacca gcgaatcacc cacagcacga ttaacgacaa tatttgggca
    19981 tcgctacaaa atgctcaaat tcaggcgttg aaaacgctcg accagcgtcc agcggagaaa
    20041 ttcgcccagc agatgtatga gacgcgctat gccgatgacc gcacgaaatt accgcaagaa
    20101 aatcagattg cacagtttac tgccgcagat gcactggctg ccgatcgcca gttgttttca
    20161 tctccagcgg atatcacgtt tgtcattgtc ggtaatgtcg cagaagacaa actcgtggcg
    20221 ttaattacgc gttacttagg atcaatcaaa cactctgatt cgccattagc cgcaggtaaa
    20281 ccattaactc gcgcgacgga caacgcatcg gttactgtaa aagaacaaaa tgaaccagtg
    20341 gcacaggttt cacagtggaa gcgttataat tcccggacac ctgttaatct gacgacgcgt
    20401 atggcgctcg atgcttttaa cgtcgcactg gcaaaagatc tacgcattaa tattcgtgaa
    20461 caggcatctg gggcatacag cgtttcttct cgcctctcgg ttgatcctca ggccaaagat
    20521 atcagtcatt tgctggcttt tacttgtcaa ccagaacgac atgatgaact gttaacgtta
    20581 gcgaatgaag tgatggttaa gcgtctggcc aaagggatta gtgagcaaga actgaacgaa
    20641 taccagcaaa acgttcagcg cagtctcgat atccaacagc gtagcgttca acaattagcg
    20701 aataccattg taaatagtct tattcaatat gacgatcctg cagcatggac tgagcaggag
    20761 caattgttga aacaaatgac ggtagagaat gttaacacta ctgttaaaca atatctttct
    20821 catccggtga atacctatac cggagtatta ttgccaaaat aatcacagcc ccgtcaacac
    20881 atcgttggcg gggattttat caatattcgc tatttttatg taataatttt ataaatgtgt
    20941 tcaa
//
LOCUS       DAZKHK010000095        20698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKHK010000095 DAZKHK010000000
VERSION     DAZKHK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.095"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            84..533
                     /gene="alaE"
                     /locus_tag="ACHOS5_003511"
     CDS             84..533
                     /gene="alaE"
                     /locus_tag="ACHOS5_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP9778270.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(570..914)
                     /locus_tag="ACHOS5_003512"
     CDS             complement(570..914)
                     /locus_tag="ACHOS5_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP9778271.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1066..1395
                     /locus_tag="ACHOS5_003513"
     CDS             1066..1395
                     /locus_tag="ACHOS5_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP9778272.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1452..2786)
                     /locus_tag="ACHOS5_003514"
     CDS             complement(1452..2786)
                     /locus_tag="ACHOS5_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9778273.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEAMARQVAQQLRKALS"
     gene            2874..3305
                     /locus_tag="ACHOS5_003515"
     CDS             2874..3305
                     /locus_tag="ACHOS5_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9778274.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRIS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3514..3759
                     /gene="nrdH"
                     /locus_tag="ACHOS5_003516"
     CDS             3514..3759
                     /gene="nrdH"
                     /locus_tag="ACHOS5_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9778275.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3756..4166
                     /gene="nrdI"
                     /locus_tag="ACHOS5_003517"
     CDS             3756..4166
                     /gene="nrdI"
                     /locus_tag="ACHOS5_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9778276.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4139..6283
                     /gene="nrdE"
                     /locus_tag="ACHOS5_003518"
     CDS             4139..6283
                     /gene="nrdE"
                     /locus_tag="ACHOS5_003518"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9778277.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQHRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGNETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFDGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQNAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6293..7252
                     /gene="nrdF"
                     /locus_tag="ACHOS5_003519"
     CDS             6293..7252
                     /gene="nrdF"
                     /locus_tag="ACHOS5_003519"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9778278.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLTNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWSDDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7609..8811
                     /gene="proV"
                     /locus_tag="ACHOS5_003520"
     CDS             7609..8811
                     /gene="proV"
                     /locus_tag="ACHOS5_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP9778279.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8804..9868
                     /gene="proW"
                     /locus_tag="ACHOS5_003521"
     CDS             8804..9868
                     /gene="proW"
                     /locus_tag="ACHOS5_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP9778280.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9925..10917
                     /gene="proX"
                     /locus_tag="ACHOS5_003522"
     CDS             9925..10917
                     /gene="proX"
                     /locus_tag="ACHOS5_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP9778281.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTAEQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEDDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11109..12293
                     /locus_tag="ACHOS5_003523"
     CDS             11109..12293
                     /locus_tag="ACHOS5_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9778282.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            12417..13154
                     /gene="ygaZ"
                     /locus_tag="ACHOS5_003524"
     CDS             12417..13154
                     /gene="ygaZ"
                     /locus_tag="ACHOS5_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP9778283.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13144..13479
                     /gene="ygaH"
                     /locus_tag="ACHOS5_003525"
     CDS             13144..13479
                     /gene="ygaH"
                     /locus_tag="ACHOS5_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP9778284.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13570..14100
                     /gene="emrR"
                     /locus_tag="ACHOS5_003526"
     CDS             13570..14100
                     /gene="emrR"
                     /locus_tag="ACHOS5_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP9778285.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14227..15399
                     /gene="emrA"
                     /locus_tag="ACHOS5_003527"
     CDS             14227..15399
                     /gene="emrA"
                     /locus_tag="ACHOS5_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP9778286.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15416..16954
                     /gene="emrB"
                     /locus_tag="ACHOS5_003528"
     CDS             15416..16954
                     /gene="emrB"
                     /locus_tag="ACHOS5_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP9778287.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17018..17533)
                     /gene="luxS"
                     /locus_tag="ACHOS5_003529"
     CDS             complement(17018..17533)
                     /gene="luxS"
                     /locus_tag="ACHOS5_003529"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9778288.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17683..19239)
                     /gene="gshA"
                     /locus_tag="ACHOS5_003530"
     CDS             complement(17683..19239)
                     /gene="gshA"
                     /locus_tag="ACHOS5_003530"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP9778289.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(19312..19740)
                     /locus_tag="ACHOS5_003531"
     CDS             complement(19312..19740)
                     /locus_tag="ACHOS5_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP9778290.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19737..20303)
                     /gene="yqaB"
                     /locus_tag="ACHOS5_003532"
     CDS             complement(19737..20303)
                     /gene="yqaB"
                     /locus_tag="ACHOS5_003532"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP9778291.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20584..20660)
                     /locus_tag="ACHOS5_003533"
     tRNA            complement(20584..20660)
                     /locus_tag="ACHOS5_003533"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20624..20626),aa:Arg,seq:acg)
BASE COUNT         4929 a         5397 c         5240 g         5132 t
ORIGIN      
        1 tatgcgaaaa tgcagatggc aatgagatcc actgctttca tctccattaa catcccatta
       61 cgcttttatt aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg
      121 cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc
      181 tctccggaat gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggtgaaca
      241 tcttaattgc atggccatac ggtatgtacc gcgatctgtt tatgcgcgcg gcacgcaaag
      301 ttagcccgtc gggctggata aaaaatctgg cggatatcct ggcttatgtg acgttccagt
      361 caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg
      421 cagtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg
      481 attattgccg ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgacccttct
      541 tttcgcgact ggcatcgcca gtcgcgtctt taactagctt cgccaaataa accattcaaa
      601 taacgttcaa gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcatgacgt
      661 tcacccgcta aatagagcgg aaattgctgg taatgatcgc aagttttaat atcggaagcg
      721 ggttcagcca gtgttgaact acgttctttt aatgttggat gaatcaccag ggcggtacgt
      781 cccattcgcg cttcgcgatt aacatagaca taattttccc cacggcggta accatacgct
      841 ttctgggtta ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc cacctcgtct
      901 ggtcgtaaat acatatttga tcctcgttat tatcaaccgc gggcctacct tacctgattg
      961 cgcattcaca acgctttcag aaaagtccat aaagccgcga tgaacctcag ttaagaaaat
     1021 tatggtctac actgaaaatt acatcgaatt ctaatggggg atcatatgtt taaccgaccg
     1081 aaccgcaacg acgttgatga tggcgtgcag gatatccaga atgatgtcaa tcaattagct
     1141 gacagtctcg aatctgtatt gaagtcctgg ggcagcgacg ccaaagggga agccgaagcc
     1201 gctcgtagta aagcgcaggc tctactgaaa gaaaccaggg cgcgaatgca tggtcgtact
     1261 cgcgtccagc aagccgcgcg cgatgccgtt ggctgcgcgg attcttttgt tcgtgaaaga
     1321 ccctggtgta gtgtgggtac agcggcagcg gtgggtattt ttatcggcgc actgttaagc
     1381 atgcgcaaat cgtagtgcaa aaatgataat aaatacgcgt ctttgacccc gaagcctggc
     1441 ttcggggttt tttagcttaa ggctttacgt aattgttgtg ctacctgtcg cgccatagct
     1501 tcgttaacga tattagtaaa cgaaagcagt aatccacctt ccccacttga acggatacgc
     1561 cagtcgctaa gtgcctgcac cgcaaggcct gcagcattgg ctttacgcgc atgggcaata
     1621 tcatcgccta tcattctgat caccatctgg ataccacctt tctgcggcac aacctgaaat
     1681 ccttgctgcg tcaacgcttg ctcaatccat tgccgacgtt gagcataatg ctgacgcatt
     1741 ttcttcagat gccgccagaa atgcccctcg cgaagaaagt ctgccagtgt gttctgccat
     1801 agaacaggta ccgcacatgg agccagtgac gcctggtggc ggaattgtgc aatttgcttc
     1861 accggcacca ccagccacgc acagcgcaat gcaggaaata gcgctttgct gaatgttccg
     1921 gcataaatta cccgctgcgg tgcgtcgaga ctttttaacg ccggtaacgg cttaccgtga
     1981 tagcgaaact cactgtcata atcatcttca ataatccatg cctgactacg atctgcccat
     2041 tccagtatct gatgcctgcg cgctaaagag agcgccacac ccagcggact ttggtgtgct
     2101 ggtgttatca gggcaaaacg cgcatcagga taattttgta ttccgcttgt gatatccagt
     2161 ccattgtcat caaccggcac aggcaaaatt tccacatcat ggcgagtgac aatcgggcga
     2221 atcagtggaa agccgggatc ttctatccac atcccgtttc ccggtttcgc cagcgcgtgc
     2281 agaattaaag ctattgaggc cgcataaccg tgagtgataa agacctgctc tggctgacaa
     2341 tcaatcccac gtgaaacgcg taagtaatca actattgcct cgcgcaacgc cgcctcaccg
     2401 cagacatcgc ctaacgccaa atcaaagcgc gtctgggtac gaaggcgacg ccccatcacc
     2461 cgcgcccaca gctctcgcgg aaagagatcg agtgcgggta atcccatttg aaatggcaac
     2521 ggttgctgac tttcaccggc aaaagctaca acttgtacag tagattgttg cggatatatc
     2581 cgctcactga caaatgtgcc agcctgtccc cgcctgatca gccatccttg cgccaccagc
     2641 tcagcatacg cattttcgac agtggatcga gacaccccca gctcctgcga ccaggtacga
     2701 cttgaaggca gccttgcgcc gggtttcagt tccccttgct caatagccgt ttttaactga
     2761 cgggcgatat cctgatagcg cggcatatgg tctgcttttt taggtaattt atggctctct
     2821 ttagcagtcc attatagtgc tacattgcgg gcgttaataa gaggagaaac tgtatgacta
     2881 cattacgtca gccttactac gaacttagcc cggcagtgta taacgcacta gtgcaggcca
     2941 aaacggcact ggaaaatagc acgctggata ccacgttgat ggagttagtt tatttgcgca
     3001 tctcgcaaat caacggctgc gcattttgtc tggagatgca cagcaaagca ttgcgcaaat
     3061 ccggcgtgcc acagcacaaa ctggacgccc tggcaggttg gcgcgtaagc catcattttg
     3121 atgaacgcga gcgggcggcg ctggcatggg cggaatcggt aaccgaaatt gccagaaccc
     3181 atgcggaaga cgaggtttat cagcctttgc ttgagcattt cagcgcagca gagatcagcg
     3241 acttaacgtt tgccatcggg ctgatgaatt gttttaaccg tctggccgtt tccatgcgga
     3301 tgtaacttct tgatgccggg tatttgcccg gcaaaaaaac acaaaatccc ctaccccgtt
     3361 gagccattca tcggtggcgt aaccaatcta aaattcccat atcttgtgtg gttgaatctt
     3421 ttttcaacta catctagtat ctctgtatca acagagagac aacccgacgc gtatcatcgc
     3481 gccgtatctt cattttaaac ggaaatacga atcatgcgca ttactattta cactcgtaac
     3541 gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accggggctt tgattttgaa
     3601 atgattaatg tcgatcgcgt tcctgaagcg gcagaagcgc tgcgtgctca gggctttcgt
     3661 cagttgccgg ttgtgattgc aggcgatctt agctggtctg gtttccgccc ggacatgatc
     3721 aatcgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc tacttttcca
     3781 gcagctccga aaatacgcag cgttttatcg aacgtttagg gctgcccgcg gtgcgcatcc
     3841 cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc gtgccctctt
     3901 acggcggcgg cggtacggct ggcgcggtgc cacgacaggt cattcgcttt ttaaacgacg
     3961 agcataaccg ggcgttgctg cgcggcgtta ttgcttcggg taatcgcaac tttggtgagg
     4021 catatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg ctgtaccgct
     4081 ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta accgaatttt
     4141 ggcaacgaca accgcagaac gcctgacgca ggaaacgatg gattatcacg ctctgaatgc
     4201 gatgcttaac ctttacgata gcgcaggtcg cattcagttc gataaagatc gccaggcagt
     4261 agacgccttt atgacgacgc atgtgcgtcc gaacagtgtg gccttcagta gccagcagca
     4321 tcgcctgaac tggctggtta acgaaggtta ctatgatgaa agtgtcctta atcgctactc
     4381 tcgcgatttt gtcattacgc tgtttgccca cgcgcacgcc agcggttttc gtttccagac
     4441 attcctcgga gcatggaagt tttacaccag ctatacgttg aagacattcg acggtaagcg
     4501 ttatctggaa gattttgccg atcgcgtcac gatggtggcg ctgacgctgg cacaaggcaa
     4561 tgaaacactg gcgacgcagc tcactgatga gatgctgtca ggacgctttc agccagccac
     4621 gccaacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct gttttctgct
     4681 gcgtattgaa gacaatatgg agtcgattgg ccgggcagtg aattccgcac tacaactgtc
     4741 gaaacgcggc ggcggcgtag catttttgct gtcgaatctg cgggaagcgg gcgcgccaat
     4801 taaacgtatt gaaaatcaat cttctggcgt gattccggtg atgaaaatgc tggaagacgc
     4861 attttcctat gccaaccaac tcggcgcgcg tcagggggct ggggcagtct atttacatgc
     4921 tcatcatccc gatattctgc gttttctcga tacaaaacgg gaaaatgccg acgaaaaaat
     4981 ccgcattaaa acactgtcgc tgggcgtggt gatcccagat atcactttcc atctggcaaa
     5041 agagaatgcg cagatggcgc tgttttcgcc ttatgacgta gagcgagttt atggcaagcc
     5101 atttgccgat gtcgccatca gtgaacacta tgacgaactg gttgccgatg aacgcattcg
     5161 caaaaaatac ctcaacgccc gtgatttctt ccagcgactg gcagagatcc agtttgagtc
     5221 tggctatccg tacatcatgt atgaagacac ggttaaccgt gctaacccta tcgccggacg
     5281 cataaatatg agcaatctct gctcagaaat tttgcaggtt aacagcgcct cagagtatga
     5341 cgagaatctc gactatgccc gtacaggcca tgatatttcc tgcaatttag gttcgttgaa
     5401 tattgcccac accatggatt cccccgactt tgcccgcacg gtagagactg ccgtacgcgg
     5461 tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg aagccggaaa
     5521 tgccgcctca cacgccatcg gtctggggca gatgaattta cacggttatc tggcgcgaga
     5581 aggcatcgct tatgggtcgc cggaagcact ggatttcacc aatctctatt tctataccat
     5641 cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg aaaccttcga
     5701 cgggttcaaa cagtcacgct atgccagtgg tgaatatttc agccaatatc tgcaaggtaa
     5761 ctggcagccg aaaacagcga aagttggcga actgtttgcc cgtagctgta ttacgttacc
     5821 tacccgtgag atgtgggcgc agttgcgcga cgacgtgatg cgctatggca tatacaacca
     5881 gaatctccag gcggtgccgc caactggttc tatctcttat atcaaccatg ccacgtcgag
     5941 tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag gacgcgttta
     6001 ctaccctgcc ccgtttatga ctaacgaaaa tctggcgctg tatcagaacg cttacgaaat
     6061 cggcgcagaa aagatcatcg acacctacgc ggaagcgact cgccatgtcg atcaggggct
     6121 gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca aagcgcagat
     6181 ttacgcctgg cgcaagggta tcaaaacgct ctattacatt cgcctgcgtc agatggcgct
     6241 ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat ctatgaaact
     6301 ctcacgtatc agcgccatca actggaataa gatatctgac gataaagatc tggaggtgtg
     6361 gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccactgtcga acgatattcc
     6421 tgcatggcag acattaagtg tcgtagaaca acaactgacg atgcgcgttt ttactggcct
     6481 gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc ccgatgcgct
     6541 cacgccccat gaagaggcgg tattaacgaa tatcagcttt atggaagcgg ttcatgcccg
     6601 ctcttacagt tcgattttct cgacgctgtg ccagaccaaa gatgtcgatg ccgcctacgc
     6661 ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc aacattatcg
     6721 cggggatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt ttttgttcta
     6781 ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctaacca ataccgcaga
     6841 cctgatccgt ctgattatcc gggatgaagc agtccacggt tattacatcg gctataaata
     6901 tcagaaaaac atggaaaaga tctcccaggc acaacgtgaa gagttgaaga gtttcgcctt
     6961 cgatttgcta ctggaacttt atgacaacga gttgcaatat accgatgagc tgtacgccga
     7021 aaccccgtgg tctgacgatg taaaagcgtt tctctgttac aacgccaata aggcgttgat
     7081 gaatctgggc tacgaaccgc tatttcccgc agaaatggcg gaagtgaatc cggcaatcct
     7141 cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt caggctcttc
     7201 atatgtgatg ggaaaagcgg tcgaaacaga agatgaagac tggaatttct gagggtgtta
     7261 tttttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata gctttttatc
     7321 acgcaaatat tttgtggtga tctacactga cactctgttg cattattcgc ctgaaaccac
     7381 aatattcagg cgttttttcg ctatctttga caaaaaatat caacttttct cgatttgctc
     7441 tcagccctta tatcatggga aattccggcg atttgctcac atcaatattc atgccacatt
     7501 tgccatcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt gactatttcc
     7561 attgggtaat ttatcgacat aaacaaataa caggaatctt tctattgcat ggcaattaaa
     7621 ttagaaatta aaaatcttta taaaatattt ggtgagcatc cacagcgagc tttcaaatat
     7681 atcgaacaag gactttcaaa agaacaaatt ctggaaaaaa ctgggctatc gcttggcgtt
     7741 aaagacgcca gtctggccat tgaagaaggc gagatatttg tcatcatggg attatccggt
     7801 tcgggtaaat ccacaatggt acgccttctc aatcgcctga ttgaacccac ccgcgggcaa
     7861 gtgctgattg atggtgtgga tattgccaaa atatccgacg ccgaactccg tgaggtgcgc
     7921 agaaaaaaga ttgcgatggt cttccagtcc tttgccttaa tgccgcatat gaccgtgctg
     7981 gacaatactg cgttcggtat ggaactggcc ggaattaatg ccgaagaacg ccgggaaaag
     8041 gcccttgatg cactgcgtca ggtcgggctg gaaaattatg cccacagcta cccggatgaa
     8101 ctctctggcg ggatgcgtca acgagtggga ttagcccgcg cgttagcgat taatccggat
     8161 atattattaa tggacgaagc cttctcggcg ctcgatccat taattcgcac cgagatgcag
     8221 gatgagctgg taaaattaca ggcgaaacat cagcgcacca ttgtctttat ttcccacgat
     8281 ctcgatgaag ctatgcgtat tggtgaccga attgccatta tgcaaaatgg tgaagtggtg
     8341 caggttggta caccggatga aattcttaat aatccggcga atgattatgt ccgaaccttc
     8401 ttccgtggcg tcgatattag ccaggtgttc agtgcgaaag atattgcccg tcgtacaccg
     8461 aacggcttaa ttcgtaaaac ccccggcttc ggcccgcgtt cggcactgaa attattgcag
     8521 gatgaagatc gcgaatatgg ctacgtcatc gaacgcggta ataagtttgt cggcgctgtg
     8581 tctatcgatt cgcttaaagc cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt
     8641 gatgcgccgt tagcggtcga tgcacaaacg cctcttagcg agttgctctc tcatgtcgga
     8701 caggccccct gtgcggtgcc cgtggtcgac gaggaccaac agtatgtcgg catcatttcg
     8761 aaaggaatgc tgctgcgcgc tttagatcgt gagggggtaa ataatggctg atcaaaataa
     8821 tccgtgggat accacgccag cggcggacag tgctgcacaa tccgcagacg cctggggtac
     8881 accggcgact gcaccgaccg acggcggtgg cgctgactgg ctgaccagta cgcctgcgcc
     8941 aaacgtcgag cattttaata ttctcgatcc gttccataaa acgctgatcc cgctcgacag
     9001 ttgggtcact gaagggatcg actgggtcgt tacccatttt cgtcccgtct ttcagggcgt
     9061 gcgcgttccg gttgattaca tcctcaacgg tttccagcaa ttgctgctgg gtatgcccgc
     9121 gccggtggcg attatcgttt tcgctctcat cgcctggcag atttccgggg tcggaatggg
     9181 cgtggcgacg ctggtttcgc tgattgccat cggcgcaatc ggtgcctggt cacaggcaat
     9241 ggttaccctg gcgctggtgt taacagccct gctgttctgt atcgtcatag gtttgccgtt
     9301 ggggatctgg ctggcgagaa gtccgcgagc ggcgaaaatt attcgtccac tgcttgatgc
     9361 catgcagacc acgcccgcgt ttgtttatct ggtgccaatc gtcatgctgt ttggtatcgg
     9421 caacgtgccg ggcgtggtgg tgacgatcat ctttgcgctg ccgccgatta tccgtctgac
     9481 gattctggga attaaccagg ttccggcgga tctgattgaa gcctcgcgct cattcggtgc
     9541 cagcccgcgc cagatgctgt tcaaagttca gttaccgctg gcgatgccaa ccattatggc
     9601 gggcgttaac cagacgctga tgctggccct ttctatggtg gtcatcgcct cgatgattgc
     9661 cgtcggcggg ctgggtcaga tggtacttcg cggtatcggt cgtctggata tggggcttgc
     9721 caccgttggc ggcgtcggga ttgtgatcct cgccattatc ctcgaccgcc tgacgcaggc
     9781 cgttgggcgc gactcacgca gtcgcggcaa tcgtcgctgg tacaccactg gccctgtcgg
     9841 tctgctgacc cgcccattca ttaagtaact ctgcacttgc ccggtgacgc cgggcatatc
     9901 accctgccaa aaaaaggaat aacaatgcga catagcgtac tttttgcgac agcgtttgcc
     9961 acgcttatct ctacacaaac ttttgctgcc gacctgccgg gcaaaggcat tactgttaat
    10021 ccagttcaaa gcaccatcac cgaagaaacc ttccagacac tgctggtcag tcgtgcgctg
    10081 gagaaattag gttataccgt caataaaccc agcgaagtgg attacaacgt tggctacacc
    10141 tcacttgctt ccggcgatgc aaccttcacc gcagtgaact ggacgccact gcatgacaac
    10201 atgtacgaag ctgccggtgg cgataagaaa ttttatcgtg aaggagtatt tgttaacggc
    10261 gcggcacagg gttacctgat cgataagaaa actgccgaac agtataaaat taccaacatc
    10321 gcgcaactta aagatccgaa gatcgccaaa ctgttcgata ccaacggcga cggtaaagcg
    10381 gatttaaccg gctgtaaccc aggctggggc tgcgaaggtg cgatcaacca ccagcttgcc
    10441 gcgtatggac tgaccaatac cgtgacgcat aatcagggga actacgcagc gatgatggct
    10501 gacaccatca gtcgttacaa agagggtaag ccggtgtttt actacacctg gacgccgtac
    10561 tgggtgagta acgaactgaa accggggaaa gatgtggtct ggttgcaggt gccgttctcc
    10621 gcactgccgg gcgataaaaa tgccgatacc aaactgccga atggcgcgaa ttacggcttc
    10681 ccggtcagta ccatgcatat cgttgccaac aaagcctggg ccgagaaaaa cccggcagca
    10741 gcgaaactgt ttgccattat gcagttacca gtggctgata ttaacgccca gaacgccatc
    10801 atgcatgacg gcaaagcctc agaagacgat attcagggcc acgttgatgg ttggatcaaa
    10861 gcccaccagc agcagttcga tggctgggtg aatgaagcgc tggcagcgca gaagtaattt
    10921 ttattcgggc ggataaggcg tttttgcctc atccgccgct ctgtacacaa tgcctgatgc
    10981 gacgctggcg cgtcttatca tacctacaaa ccacaccgca catttcaaca atctatcttt
    11041 catcccatat tcatcaacat ccgctattat tgatttccag cttaatcatc acctgatgaa
    11101 caaaaataat gactaaacct aatcatgagc ttagcccggc gctgatcgtg ctgatgtcta
    11161 tcgccaccgg tctggcggta gccagtaact attacgccca gccattgctc gacaccatcg
    11221 cgcgtaactt ttccctttcc gccagttcgg caggctttat tgttaccgcc gcgcagttgg
    11281 gctatgccgc aggtctgctg tttcttgttc ccctcggtga tatgtttgaa cgccgccgcc
    11341 tgattgtctc gatgacctta ctggcagcag gcggcatgtt aatcactgcc agcagtcagt
    11401 cgctggcgat gatgatcctc ggtacagcat taaccggttt attttcagtc gtggcacaaa
    11461 ttctggttcc actggcagcg acgctggctt caccggacaa acgcggcaaa gtggttggca
    11521 ccattatgag cggtctgcta ttggggatct tactcgcacg gaccgttgcc ggattgctgg
    11581 cgaatctcgg cggctggcgc accgtctttt gggttgcttc gatgttaatg gcactgatgg
    11641 cgctggcatt atggcgtggt ctgccacaaa tgaaatcaga aacccacctc aactacccac
    11701 agttgttggg ttccgttttc agcatgttta tcagcaataa gatcctgcgc acccgcgcgt
    11761 tgctgggctg cctgaccttt gccaacttca gcattctctg gacctcaatg gcctttttgc
    11821 ttgccgctcc accttttaac tacagcgatg gtgtaattgg tctgtttggg cttgcgggag
    11881 ctgccggagc gttgggcgct cgtccggcgg gcggttttgc cgataagggc aaatcgcacc
    11941 acaccacaac tttcggtctg ctgctgctgt tactttcatg gctggcaatc tggtttggtc
    12001 acacttccgt actggcgctg attatcggca tcctggtgct ggacctcacc gtgcagggcg
    12061 tgcatatcac taaccagacg gtaatttatc gaatacaccc tgatgcgcgt aatcgcctga
    12121 ccgcaggtta catgaccagc tactttattg gcggtgccgc tggttcgcta atttcagcct
    12181 cagcctggca acatggcggt tgggctggcg tgtgtctggc tggcgcgacg attgccctgg
    12241 ttaacttact ggtctggtgg cgaggttttc atcgtcagga agccgcaaat taagcaatca
    12301 gcaaccgttt tcgtggcttt acacttataa gggtgttaag aagcccatca gtctgataag
    12361 gttaagatat tcattcagtc tatttataat attaacaatc gttaagcgta cactctatgg
    12421 aaagccctac tccacagcct gctcctggtt cggcgacctt catggaagga tgcaaagaca
    12481 gtttaccgat tgttattagt tatattccgg tggcctttgc gttcggtctg aatgcgaccc
    12541 gtctgggatt ctctcctctc gaaagcgttt ttttctcctg catcatttat gcaggcgcga
    12601 gccagttcgt cattaccgcg atgctggcag ccgggagtag tttgtgggtt gctgcactga
    12661 ccgtcatggc aatggatgtt cgccatgttt tgtatggccc gtcactgcgt agccgtatta
    12721 ttcagcgtct gcaaaaatcg aaaaccgccc tgtgggcgtt tggcctgacg gatgaggttt
    12781 ttgccgccgc aaccgcaaaa ctggtacgca ataatcgccg ctggagcgaa aactggatga
    12841 tcggcattgc cttcagttca tggtcatcgt gggtatttgg tacggtaata ggggcattct
    12901 ccggcagcgg cttgctgcaa ggttatcccg ccgttgaagc agcattaggt tttatgcttc
    12961 cggcactctt tatgagtttc ctgctcgcct ctttccagcg caaacaatct ctttgcgtta
    13021 ccgcagcgtt agttggtgcc cttgcaggcg taacgctatt ttctattccc gtcgccattc
    13081 tggcaggcat tgtctgtggc tgcctcactg cgttaatcca ggcattctgg caaggagcgc
    13141 ccgatgagct atgaggttct gctgcttggg ttactggttg gcgcggcgaa ttattgcttc
    13201 cgctatctgc cgctgcgcct gcgtgtgggt aatgcccgcc caaccaaacg tggcgcggta
    13261 ggtattttgc tcgacaccat tggcatcgcc tcgatatgcg ctctgctggt tgtctctacc
    13321 gcaccagaag tgatgcacga tacacgccgt ttcgtgccca cactggtcgg cttcgcggta
    13381 ctgggtgcca gtttctataa aacacgcagc attatcatcc caacactgct tagtgcgctg
    13441 gcctatgggc tagcctggaa agtgatggcg attatataac ccacaagaat catttttcta
    13501 aaacaataca tttactttat ttgtcactgt cgttactata tcggctgaaa ttaatgaggt
    13561 catacccaaa tggatagttc gtttacgccc attgaacaaa tgctaaaatt tcgcgccagc
    13621 cgccacgaag attttcctta tcaggagatc cttctgaccc gtctttgcat gcatatgcaa
    13681 agcaagctgc tggaaaaccg caataaaatg ctgaaggctc aggggattaa cgagacgttg
    13741 tttatggcgt tgattacgct ggagtctcag gaaaaccaca gtattcagcc ttctgaattg
    13801 agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta ttgccgatga actggaaaaa
    13861 cgcggttgga tcgaacgtcg tgaaagcgat aacgatcgcc gctgcctgca tctgcaatta
    13921 acggaaaaag gtcacgagtt tttgcgcgag gttctaccgc cgcagcataa ctgcctgcat
    13981 caactctggt ccgcgctcag cacaacagaa aaagaccagc tcgagcaaat cacccgcaaa
    14041 ttgctctccc gtctcgacca gatggaacag gacggtgtgg ttctcgaagc gatgagctaa
    14101 cgcgtcatct cgctcaaaaa tccagattta taaaagaaaa aataaccggc cagcatcgca
    14161 acatgctggc ctttttggca agcaggtcgg ctcagccgat gagttaagaa gatcgtggag
    14221 aacaatatga gcgcaaatgc ggagactcaa accccgcagc aaccggtaaa gaagagcggc
    14281 aaacgtaagc gtctgctcct ccttctcacc ttgctcttta taattattgc cgtagcgata
    14341 gggatttatt ggtttttggt actgcgtcat ttcgaagaaa ccgatgacgc atacgtggca
    14401 gggaatcaga ttcaaattat gtctcaggtg tctggcagcg tgacgaaagt ctgggccgat
    14461 aacaccgatt ttgtaaaaga aggcgacgtg ctggtcactc tcgacccgac agatgctcgc
    14521 caggcatttg aaaaagccaa aactgcactg gcttccagcg ttcgccaaac ccaccaactg
    14581 atgattaaca gcaaacagtt gcaggcgaat attgaggtgc aaaaaatcgc cctcgcgaaa
    14641 gcacaaagcg actacaaccg ccgtgtgccg ctgggcaatg ccaacctgat tggtcgcgaa
    14701 gagctgcaac acgcccgcga cgccgtcacc agtgcccagg cgcaactgga cgtcgctatt
    14761 caacaataca atgccaatca ggcgatgatt ctggggacga aactggaaga tcagcctgcc
    14821 gtacaacagg ctgccaccga agtacgtaac gcctggctgg cgctggagcg tacccgtatt
    14881 gtcagtccga tgaccggtta tgtctcccgc cgcgcggtac agcctggggc gcaaattagc
    14941 ccaacgacgc cgctgatggc ggtcgttcca gccaccaata tgtgggtgga tgccaacttt
    15001 aaagagacgc agattgccaa tatgcgtatc ggtcagccgg tcaccatcac caccgatatt
    15061 tacggcgatg atgtgaaata caccggtaaa gtggttggtc tggatatggg cacaggtagc
    15121 gcgttctcac tgcttccggc gcaaaacgcc accggtaact ggatcaaagt cgttcagcgt
    15181 ctgcctgtgc gtattgaact ggaccagaaa cagctggagc aatacccgct gcgtatcggt
    15241 ttatccacgc tggtgagtgt caataccact aaccgtgacg gtcaggtact ggcaaataaa
    15301 gtacgttcca caccggtcgc ggtaagcacc gcgcgtgaaa tcagcctggc acctgtcaat
    15361 aaactgatcg acgatatcgt aaaagctaac gctggctaat ccagaggtgc gtgtgatgca
    15421 acagcaaaaa ccgctggaag gcgcacaact ggtcattatg acgattgcgc tgtcactggc
    15481 gacattcatg caggtgctgg actccaccat tgctaacgtg gcgatcccca ctatcgccgg
    15541 gaatctgggc tcatcactca gtcagggaac gtgggtgatc acttctttcg gggtggcgaa
    15601 tgccatctcg atcccgctta ccggctggct ggcaaagcgc gtcggggaag tgaaattgtt
    15661 cctctggtca accatcgcct ttgctattgc gtcgtgggcg tgtggtgtct ccagcagcct
    15721 gaatatgctg atcttcttcc gcgtgattca ggggattgtc gccgggccgc tgatcccgct
    15781 ttcgcaaagt ctattgctga ataactaccc gccagccaaa cgctcgatcg cgctggcgtt
    15841 gtggtcgatg acggtgattg ttgcgccaat ttgcggcccg atcctcggcg gttatatcag
    15901 tgataattac cactggggct ggatattctt catcaacgtg ccgattggcg tggcggtggt
    15961 gttgatgaca ctgcaaactc tgcgcggacg tgaaacccgc accgaacggc ggcggattga
    16021 tgccgtgggg ctggcactgc tggttattgg tatcggcagc ctgcagatta tgctcgaccg
    16081 cggtaaagag ctggactggt tctcatcaca ggaaattatc atcctcaccg tggtggcggt
    16141 ggtggctatc tgcttcctga ttgtctggga gctgaccgac gataacccga tagtcgatct
    16201 gtcgttgttt aagtcgcgca acttcaccat cggctgcttg tgtatcagcc tcgcgtatat
    16261 gctctacttc ggcgcgattg ttctgctgcc gcagttgttg caggaggtct acggttacac
    16321 ggcgacgtgg gcagggctgg cctctgcgcc ggtagggatt attccggtga tcctgtcgcc
    16381 gattatcggc cgctttgcgc ataaactgga tatgcgccga ctggtaacct tcagctttat
    16441 tatgtatgcc gtctgcttct actggcgtgc ctataccttt gaaccgggta tggattttgg
    16501 cgcgtcggcc tggccacagt ttatccaggg ttttgcggtg gcctgcttct ttatgccgct
    16561 gaccaccatt acgctgtccg gtttgccacc ggaacgactg gcggcggcat caagcctctc
    16621 caactttacg cgaacgctgg cggggtctat cggcacgtcg ataaccacga ccatgtggac
    16681 caaccgcgag tcactgcacc atgcacagtt gactgagtcg gtaaacccgt tcaacccgaa
    16741 tgctcaggcg atgtacagtc aactggaagg gcttggtatg acgcaacagc aggcatcagg
    16801 ctggattgcc cagcagataa ccaatcaggg gctgattatt tccgccaatg agatcttctg
    16861 gatgtcagcc gggatattcc tcgtcctgct ggggctggtg tggtttgcta aaccgccatt
    16921 tggcgcaggt ggcggcggag gtggtgcgca ctaagtacaa ctaagccagt tcatttgaac
    16981 tggctttttt caattaattg tgaagatagt ttactgacta aatgtgcagt tcctgcaact
    17041 tctctttcgg cagcgccagt tcttcgttgc tgttgatgcg cacgtcacgt tccagaatgc
    17101 tacgcgcaat atcctgcgct tcctgcaacg agtgcatctg gtaagtgcca cactggtaga
    17161 cgttcagttc cgggatctga ttctgatcct gcactttcag cacgtcttcc attgccgctt
    17221 tccaggcatc agcgacacgc tgctcatctg gcgtaccaat cagactcata taaaaaccgg
    17281 tgcggcagcc cattggcgag atatcgataa tctctacacc attaccgtta agatggttac
    17341 gcataaaacc agcaaacagg tgctccaggg tatggatccc tctttctggc atcacttctt
    17401 tgttcggcac gcagaagcgc agatcgaaca cggtgattgc gtcgccatgc ggggtgttca
    17461 ttgtttttgc cacccgaact gcaggcgctt ccatccgggt atgatcgact gtgaagctat
    17521 ctaacaacgg catttagcca cctccggtaa tttttttaaa aattttctga actctttgtt
    17581 cccaggcgag tctgagtata tgaaagacgc gcatttgtta tcatcatccc tgaattcaga
    17641 gatgaaattt tggccactca cgagtggcct ttttcttttc tgtcaggcgt gcttttccag
    17701 ccacaccgca aacggttcgg tatcagcggc ttccatttcc tgctgacggc gttcagacgc
    17761 ctcgcgctcg gcgacaaaat cctcttcgcg cagaatttcc agcggctctt cacgcagcag
    17821 attacggtac gcttcagcaa acgctttgcc tgttccgcca ataccagtat caatcataga
    17881 ccttaagata cgggcagaga aagtcagatc aggattatcg aagcaggcga ccagttcatc
    17941 acacactttc tgatacgctt cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt
    18001 cagatcgcgg aacagatctt tacccacctg cggtaacggg aactgcgcag tttcgcagcc
    18061 gatacccagc gtcaggcccg gtttgcgacc ttcgaggatc acccggttcc agttaacgcg
    18121 tgtacaggca agttcgctac tgctcatttc cggtgcatca gccagcgcac accagaccat
    18181 aaacaggtcg aggaatcgca cctgttgttc atctacacca atcggcgaga acgggttgat
    18241 gtccagcgag cggacttcaa tatattcaat gccgccacgt aacagcgcat cagaaggcga
    18301 ctcaccactg cgggtaacgc gttttggacg aatcggcgcg tacagttcgt tttcaatctg
    18361 cagcacgttg ctattgattt gcagtcgctt accgtctttc tcaataccaa tcttcgcgta
    18421 ctcttccgat ggcgttttga ttgcctgttt aaggcccgcc acgtactcgt aaagatcgtt
    18481 gaaggtaata ccaagattgc tttgcgattt attggtatag ccaagatcgc tcaaacggag
    18541 agaggtcgca tacggcaggt aatacatacc gcactcggtt ttctcaaacg gcagcgacgt
    18601 tggttttcct tgcaggaaag aagaacaaat cgccggagac gcgccaaaca gataaggaat
    18661 gacccaaccg aaacgatagt aattgcggat aacgcggaaa tagcccgcag aaattttctc
    18721 tttggcatca gcgcccgaga tatcaccaca cttcgcttgc cagaatgcca ttggcaaaga
    18781 gaaattatag tgcacgccgg aaatggtttg catcagcgcg ccgtagcgat ttttcagccc
    18841 ttcacggtac agcgttttaa agcgtccggt gttagaagtg ccgtactgtg ccagttcgat
    18901 gtcctgacct tctgcgatgt agcatggcat acttaacggc cacatccgct catcgcccat
    18961 attgcgcgcc gtataacgat gcagatcgcg cataaaggtc aacatatgtt caatatcacc
    19021 atccactggt gtaatgaatt ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg
    19081 cgtcagtgcg gaacctaatg cttcaggatg acctgttgtt gccagtgtgc catcagcatt
    19141 aacacgcaaa gtttcgcgct ccagcccacg ctgtatcccc tttaacgcct gaggatgttt
    19201 ttccagccag gccagcgcct gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa
    19261 atcgttttaa ttagcataac tgtaatggtg accatatgtg caggcctaca attagtgcca
    19321 ccacatcatg ccctgaacgg tagctgctgc aactgcaaca tagcgtaacg ctttaccaag
    19381 gcacaaaaaa aagattaccg gtccccacga gatgcgcatc catcccgcta acagacacag
    19441 taaatcgcca accacgggca tccagcttaa taatagcgtg actgcaccat agcgtttcag
    19501 ccagccggta gctttctctt gccagcgcga tgttttacgc aatggaaaga aacgcccaag
    19561 gataacgtta gttaaccctc caaggctatt acccattgtt gctgttaaaa ctaaaaccca
    19621 gggatgactc actccggaaa gcaacattgc caccagcacg acttcggagt tgccgggtaa
    19681 tagtgtagcg ctgagaaaac tactggcaaa caacgagaaa agcgataacg cttcactcac
    19741 agcaagcgaa catccacggc gtccatgcct gctgcacggg ccgcctgaat accgaaatcg
    19801 gcatcttcaa agaccacaca ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa
    19861 aatgtgtctg gcgcgggttt atggtgtttg acgtgatcgg cagcgacgac ggcgtcaaaa
    19921 taacggcgta atcccaggtg tgccagcaat gcctcagcga tggcgctttc actccccgtt
    19981 cctacagcca ttgggcgacg accatgccag cttttcacca cttcaacaag aggaagcggc
    20041 tcgacactat ccagcagcat acttcttact gcttctgttt tttcacgcgc taacgcatgc
    20101 gggtcgagat cggcctgatt cagctcaata atcgcctgag caatacgcca ggtgggcgag
    20161 ccattaagcg caatcatcgc ctgaacatcg tactgaagac cgtagtgccc taatacttcg
    20221 cgccacgctt tacggtgcgt aggctcggta tccaggatgg tgccatccat atcaaaaatt
    20281 aaacctgcat aacgctcgta catggtcttc tcgcaagtag aaaaatatga cgctacttta
    20341 gcgtaattgc ttgattttgt cgctgatgga gaaggagaag aaaattcagg aaatggataa
    20401 agtagttatt tagaaatgag atatttttga aggaaatttt ttggagaata tggtgcatcc
    20461 gggaggatga ctcactggcg ttcgcccttc gggccgctgc tggcgcaacg ttatcctccc
    20521 tggtgcctgc ggttaatacc gctacctctt cgataccttc attgctgaag gttacggaga
    20581 atatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
    20641 tccagctgag ctacggatgc atcggaaaaa tttattttac tgctgatatt gatatcgc
//
LOCUS       DAZKHK010000096        20376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKHK010000096 DAZKHK010000000
VERSION     DAZKHK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.096"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..826
                     /gene="pdeC"
                     /locus_tag="ACHOS5_003534"
     CDS             <1..826
                     /gene="pdeC"
                     /locus_tag="ACHOS5_003534"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9778292.1"
                     /translation="IFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKNQLCLY
                     YQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQVTDYVIDNVFRD
                     LGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKFEVTEHAFLDVD
                     KMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLI
                     AEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMG"
     gene            complement(862..1185)
                     /gene="soxS"
                     /locus_tag="ACHOS5_003535"
     CDS             complement(862..1185)
                     /gene="soxS"
                     /locus_tag="ACHOS5_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP9778293.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1271..1735
                     /gene="soxR"
                     /locus_tag="ACHOS5_003536"
     CDS             1271..1735
                     /gene="soxR"
                     /locus_tag="ACHOS5_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP9778294.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2282..3631
                     /gene="ghxP"
                     /locus_tag="ACHOS5_003537"
                     /gene_synonym="yjcD"
     CDS             2282..3631
                     /gene="ghxP"
                     /locus_tag="ACHOS5_003537"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP9778295.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3782..5431
                     /gene="yjcE"
                     /locus_tag="ACHOS5_003538"
     CDS             3782..5431
                     /gene="yjcE"
                     /locus_tag="ACHOS5_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9778296.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQYIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5467..6351)
                     /locus_tag="ACHOS5_003539"
     CDS             complement(5467..6351)
                     /locus_tag="ACHOS5_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9778297.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            6455..6865
                     /locus_tag="ACHOS5_003540"
     CDS             6455..6865
                     /locus_tag="ACHOS5_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9778298.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANQVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            6858..7547
                     /locus_tag="ACHOS5_003541"
     CDS             6858..7547
                     /locus_tag="ACHOS5_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFP9778299.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(7586..9235)
                     /gene="actP"
                     /locus_tag="ACHOS5_003542"
     CDS             complement(7586..9235)
                     /gene="actP"
                     /locus_tag="ACHOS5_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP9778300.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9232..9546)
                     /gene="yjcH"
                     /locus_tag="ACHOS5_003543"
     CDS             complement(9232..9546)
                     /gene="yjcH"
                     /locus_tag="ACHOS5_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP9778301.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9838..11796)
                     /gene="acs"
                     /locus_tag="ACHOS5_003544"
     CDS             complement(9838..11796)
                     /gene="acs"
                     /locus_tag="ACHOS5_003544"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP9778302.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGTTELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12188..13624
                     /gene="nrfA"
                     /locus_tag="ACHOS5_003545"
     CDS             12188..13624
                     /gene="nrfA"
                     /locus_tag="ACHOS5_003545"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP9778303.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13669..14235
                     /gene="nrfB"
                     /locus_tag="ACHOS5_003546"
     CDS             13669..14235
                     /gene="nrfB"
                     /locus_tag="ACHOS5_003546"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP9778304.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14232..14903
                     /gene="nrfC"
                     /locus_tag="ACHOS5_003547"
     CDS             14232..14903
                     /gene="nrfC"
                     /locus_tag="ACHOS5_003547"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP9778305.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14900..15856
                     /gene="nrfD"
                     /locus_tag="ACHOS5_003548"
     CDS             14900..15856
                     /gene="nrfD"
                     /locus_tag="ACHOS5_003548"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP9778306.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15876..17594
                     /gene="nrfE"
                     /locus_tag="ACHOS5_003549"
     CDS             15876..17594
                     /gene="nrfE"
                     /locus_tag="ACHOS5_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP9778307.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLATLLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     GALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGALLSGWR
                     GRKRDE"
     gene            17587..17970
                     /gene="nrfF"
                     /locus_tag="ACHOS5_003550"
     CDS             17587..17970
                     /gene="nrfF"
                     /locus_tag="ACHOS5_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP9778308.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17967..18563
                     /gene="nrfG"
                     /locus_tag="ACHOS5_003551"
     CDS             17967..18563
                     /gene="nrfG"
                     /locus_tag="ACHOS5_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP9778309.1"
                     /translation="MKQPQIPVKMLTTLTIFMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18906..20219
                     /gene="gltP"
                     /locus_tag="ACHOS5_003552"
     CDS             18906..20219
                     /gene="gltP"
                     /locus_tag="ACHOS5_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP9778310.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
BASE COUNT         4440 a         5168 c         5686 g         5082 t
ORIGIN      
        1 tatttttgcg ttgcccgccg gtattttggg gagtcttgtt ctgctattac tctggctacg
       61 tattcgacaa aactatttgt ctcccaaacg caaattgcaa cgcgccctcg aaaaaaatca
      121 actttgcctt tattaccagc caataatcga tatcaaaaca gaaaaatgta tcggcgctga
      181 agcgttgtta cgttggcctg gtgagcaggg gcaagtaatg aatccggcag agtttattcc
      241 gctggcagaa aaggagggga tgatcgaaca ggtaactgat tatgttattg ataatgtctt
      301 ccgtgatttg ggcgcgtatc tggcaacaca taccgatcgc tatgtttcta ttaacctgtc
      361 agcctccgat tttcatacct cacggttgat agcgcgaacc aatcagaaaa cagagcaaca
      421 tgcggtgcgc ccgcagcaaa ttaaatttga agtgactgaa catgcatttc ttgatgtcga
      481 caaaatgacg ccgattattc tggctttccg caaggccggt tacgaagtgg caattgatga
      541 ttttggtatt ggctactcta acttgcataa ccttaaatca ttgaatgtcg atattttgaa
      601 aatcgataaa tcgtttgttg agacgctgac cacccacaaa accagtcatt tgattgcgga
      661 acacatcatc gagctggcgc acagcctggg gttaaaaacg atcgctgaag gtgtcgaaac
      721 tgaggcgcag gttaactggc tgcgcaaacg cggcgtgcgc tattgccagg gatggttttt
      781 tgcgaaggcg atgccgccgc aggtgtttat gcaatggatg gggtaattac ccgcgcggga
      841 gttaacgcgc gggcaataaa actacaggcg gtggcgataa tcgctgggag tgcgatcaaa
      901 ctgccgacgg aaaacgcggg agaaggtctg ctgagagaca taacccaggt ccattgcgat
      961 atcaaaaatc ggacgctcgg tggtgcgcaa ctcaacggcg gccagtaaca gacggcgctg
     1021 gcgaatgtaa tcgccaagcg tctgatgcgt caccgtgcgg aacatccgtt gcaagtacca
     1081 ctttgaatag ccagattttt ttgcgacgac atcaatgtta agcggctggt caatatgctc
     1141 gtcaatccat gcgataagat cctgaataat tttctgatgg gacataaatc tgcctctttt
     1201 cagtgttcag ttcgttaatt catctgttgg ggagtataat tcctcaagtt aacttgaggt
     1261 aaagcgattt atggaaaaga aattaccccg cattaaagcg ctgctaaccc ccggcgaagt
     1321 ggcgaaacgc agcggtgtgg cggtatcggc gctgcatttc tatgaaagca aagggttgat
     1381 taccagtatc cgtaatagcg gcaatcagcg gcgatataaa cgtgatgtgt tgcgatatgt
     1441 cgcaattatc aaaattgctc agcgtattgg cattccgctc gcaaccattc gtgaagcgtt
     1501 tggcgtgttg ccagaagggc atacgttaag tgcgaaagag tggaaacagc tttcgtccca
     1561 atggcgagaa gagttggatc ggcgcattca taccttagtg gcgctgcgtg acgaactgga
     1621 tggatgtatt ggttgtggct gcctttcgcg cagtgattgc ccgttgcgta acccgggcga
     1681 ccgattagga gaagaaggta ccggcgcacg cttactggaa gatgaacaaa actaaagcgc
     1741 cacaagggcg ctttagtttg ttttccggtc tttgtctttc tctctatccc gctggtacac
     1801 aggagggttt cccccgacgt caacacacct cattcgagca cgtggtggag gttccggttg
     1861 gtgttgatgc tttaattgta tgttaccggc gtttcttcgc cagtgtaaaa gtacactttt
     1921 tagccgcaat atttttgtca tctcagacga ttttttatcg caatcctgaa cggtatacgg
     1981 ctcgataacg ctgcaatctt gcgcatcgac gataacgttt gcgcatcaat tgcctggttt
     2041 ttcatcgtca agacaataaa agagaaaaaa gcagcaaact tcggttgaaa aagccgctat
     2101 gatcgccgga taatcgtttg ctttttttac cacccgtttt gtatgcgcgg agctaaacgt
     2161 ttgctttttt tgcgacgcag caaattgtcg caaacctgga gcagaaagat aacgtttcgc
     2221 tggcagggga ttgtccgcca cgcatcttga cgaaaattaa actctcaggg gatgttttct
     2281 tatgtctacg ccatcagcgc gtaccggcgg ttcactcgac gcctggttta aaatttcaca
     2341 acgtggaagc actgtccgtc aggaagtggt tgccgggtta acaacgtttc tggcgatggt
     2401 ctactcagtt atcgtcgttc caggcatgtt gggtaaagcg ggcttcccgc ctgcggcagt
     2461 tttcgttgca acctgtctgg ttgccggact cggttctatc gtgatgggtc tgtgggctaa
     2521 tctgccgttg gcgattggtt gcgccatctc tctgacggcg tttaccgcat tcagtctggt
     2581 gctggggcaa catattagcg tacctgtcgc gctgggtgcc gtgttcctga tgggtgtgct
     2641 gtttacggta atttctgcca cgggtatccg tagctggatt ttgcgcaact tgccgcacgg
     2701 tgtggcgcac ggcacgggga ttggtatcgg tctgttcctg ctgctgattg ctgctaatgg
     2761 tgtcggtctg gtgattaaaa acccacttga tggtctgccc gtcgcgctgg gggatttcac
     2821 gacctttccg gtgatgatgt cgttggtggg gctggcagtg attatcggcc tggaaaaact
     2881 gaaagtccct ggtggcattc tgctgaccat tatcggtatc tcaattgtcg gtttgatctt
     2941 cgatcctaac gtccatttct ccggcgtttt cgccatgcct tcattgagcg atgaaaacgg
     3001 caattcactg ataggcagcc tggacattat gggtgcgctg aatcctgtag tcctgccaag
     3061 tgttctggca ctggtgatga cggcagtatt tgatgccacc ggaactatcc gtgccgtcgc
     3121 cggtcaggcg aacctgctgg ataaagatgg gcagatcatc gacggcggga aagcactgac
     3181 caccgactcc atgagcagcg ttttttctgg cctggtgggt gcagctccgg cagcggtata
     3241 catcgagtct gcggcgggta cggcggcggg cggtaaaacc ggtttgacgg ctatcaccgt
     3301 tggcgtgctg ttcctgctga ttctgttcct ctctccgctc tcttacctcg ttccggggta
     3361 cgcaacggct ccggcgctga tgtacgttgg cctgctgatg ctgagcaacg tggcgaaaat
     3421 cgactttgct gattttgttg atgcgatggc gggtctggtt acggcggtat tcatcgtgct
     3481 gacctgtaac atcgtaacag gcatcatgat cggcttcgcg actctggtga ttggtcgtct
     3541 ggtttccggc gaatggcgca agttgaacat cggtacggtc gttattgccg tggcgctggt
     3601 gaccttctat gcgggtggct gggctatcta atcttacata ggattctgaa aacgggtggc
     3661 aatggctgcc cgtttttatt ttctccgcac attgtgtgag cttttgcgat attctgaaaa
     3721 aatgagaatt caggcataac gcctttctca gatcataaaa aacatcgcaa acagggaacg
     3781 catggaaatc ttcttcacca tactgataat gaccctcgtg gtctcgctgt ccggggtggt
     3841 cactcgtgtc atgccctttc agatcccgct tccgcttatg caaatcgcca tcggtgcgct
     3901 actggcgtgg ccgacgtttg gtttgcatgt ggagtttgat cctgaactct ttttagtctt
     3961 gtttatcccg ccgttgctgt tcgctgatgg ctggaaaacg ccgacccgtg aatttctcga
     4021 acatgggcga gagattttcg gcctcgcgct ggcgctggtg gtggtcaccg tagtcggcat
     4081 tggcttcctt atttactggg tggtgccggg cattccgctg atccccgcct ttgcgctggc
     4141 tgcggtgctt tctccgaccg atgctgtagc gctctccggg attgttggcg aggggcgcat
     4201 cccgaaaaaa atcatgggca ttttgcaggg cgaagcgttg atgaatgacg cctccggcct
     4261 ggtgtcgttg aagtttgccg tggcagtggc gatggggaca atgatcttca ccgtcggcgg
     4321 tgcgacggtc gaatttatga aagtggcaat aggcggtatt ctcgccggtt ttgtggtgag
     4381 ctggctgtat ggtcgctcgc tgcgattcct cagccgctgg ggcggtgatg aacccgcgac
     4441 gcagatcgtt ctgctgttct tgctgccatt cgcttcttat ctgattgcgg aacatattgg
     4501 cgtttccggc atcctcgctg cggttgccgc cgggatgacc atcacccgct ccggtgtgat
     4561 gcgccgtgcg ccgctggcaa tgcgcctgcg cgcaaacagc acctgggcga tgctggaatt
     4621 tgtctttaac ggcatggtgt tcctgctgtt aggtctgcag ttgccgggga ttctggagac
     4681 gtcgctgatg gcggcagaaa tcgaccctaa cgtcgaaatc tggatgctgt ttaccgatat
     4741 tattctgata tatgcggcgc tgatgctggt ccgtttcggc tggctgtgga cgatgaaaaa
     4801 gttcagcaac cgcttcctga agaagaagcc gatggagttt ggttcgtgga ctacacgaga
     4861 aatcctgatc gcgtctttcg ccggggtgcg tggggcgatc actctggccg gtgtgctctc
     4921 tatcccgctt ctcttgccgg atggtaacgt cttccctgcg cgctatgagt tagtgttcct
     4981 ggcggctggc gtcattctct tctcattgtt tgtcggcgtg gtgatgttgc ctattctgct
     5041 acaatacata gaagtcgcgg accattcgca acaattgaaa gaggaacgta ttgcacgagc
     5101 ggcaacggca gaagtggcga ttgtggcgat ccagaaaatg gaggagcgtc tggcggcgga
     5161 taccgaagag aatatcgata accagctgct cacagaggtt agttctcgcg tcattggtaa
     5221 cctgcgtcgt cgcgccgatg gacgtaatga tgtagaaagt tcaatcctgg aagagaacct
     5281 ggagcgtcgc ttccgtctgg cggcattgcg ttctgaacgt gctgaactgt accacctgcg
     5341 cgccacgcgg gagatcagta acgaaacgct gcaaaaatta ctgcacgatc tcgatttgct
     5401 tgaagcgttg ctgattgagg aaaatcagta atctgcatgg ttggcgggcg agcccgccaa
     5461 cccgacttac tctggcgtta accagtatcc ttcacaacag ctcagccacg cgtgggcaga
     5521 gtgggaaagg taaacgccct cacgccaaat cattcctaat ttccagctta gttcgctttc
     5581 cagcggtagc cattttaagg tgtttttatc taatcgctgg caaatgggtt ccggcaatat
     5641 ggctattccg acaccggcct gaaccatcgc ggcgagaaaa tcccattgcc cgctgcgaac
     5701 tgcaattctc ggcttaacgc catgttcatc gaataacttc atcaattgct tacttaaggc
     5761 aaaatcttcg ttatagataa gtagaggatg ttccgccaat gtctccggtg agacggcatc
     5821 acattcaagc catttacctg aatgtggcgt cagtacacat agtggatgac taaacagcga
     5881 taacgttgtc aggccgcttt cttctactgg cagggctgtc agtgccatat ccagttcacc
     5941 gttcatcacc gcttgttgta ccgtcagacc accaaactcc gaaattttca attctacgcc
     6001 ggggtaacgc tggcgaaaaa ggccaatcgg cccggccatc aacatcccaa ccatcggcgg
     6061 gattcccagc cgcaaaacgc cggttttaag ctgattgata tcgctaagct cagcttccag
     6121 ttgccgaaac tcagccagta aagccagccc gcgctcaaat acgacgcgac cagtatcagt
     6181 gagaagcagc cgacgaccat cgcggatcaa caatatacag ttcagctcat cttcgagatt
     6241 cttcagcatc ttgctgatag ttggttgggt gacaaacaac ttctccgctg cgcgggtgaa
     6301 gctttgctgg cgcacgactt cgacaaaata acgcagcgtt ctgatatcca taactattcc
     6361 ctttgactat acctgcaatg attttaattc atttcagtca ggggcgagcg ctctctatac
     6421 ttaccattct gttttatttt caggatatcc tctcatgccc gtggcgcttc gccgcattac
     6481 ccctgccgta gtgcaatcta tccaggtatt ttttcaggtt gtgctgtatg caggtttatt
     6541 tatttttgcg cagtatctcg tctcctggct gcatttgccg ctgcctgcca accaggtggg
     6601 gatgatcctg atgctggcgc tgattgtttg tcgcattctt cctctgcaat gggttcgagc
     6661 cggggcgcgt tggctattgg ccgaaatgtt gctgtttttt gtccctgccg tggtggctgt
     6721 ggtgaactat gcacaactat tactggtgga tggctggcgt atttttgctg tgatcgcgct
     6781 gagtacggtg atggtgctgg gcacgactgc ctgggtagtg gaaaaagtct atcgctatga
     6841 aatgcgcagg ttaaaccgtg gctaattttc agctaagtat gctctgtctg gcaattacgc
     6901 tagttatcta ttttcttaac aaacgccttt atcgccgctt tcgtaaattg ccattgatgc
     6961 ctctggtgct gacgcctacg ctactggtta tgctgctggt ttttggcaat atttcttggc
     7021 aaaactatat tggcgaagcg cactggttac tatggctatt aggtccggcc accatcgcct
     7081 ttgcggtacc agtgtatgac aatcgggcga tcattaagcg tcactggatg tcgttgacgg
     7141 caggcgtact gacggcaacg gtggtggcag tgaccagctc agtgtggctg gcgcgattgt
     7201 ttaccctgcc agatgagatc cagcgtagcc tggcggtgcg ttcggtcact acgccatttg
     7261 ctttggcagc gagccagtcg ctcggcggac agcctgatct ggtggcgctg tttgtggtgg
     7321 tcaccggtgt gtttggcatg gcgattggcg acgtgttgtt tttacgtctc tctattcgcg
     7381 aagggatggc gaaaggtgct ggattcggcg cggcatctca tggtgccggg actgcgcggt
     7441 cgtatgaact gggtcaacaa gaaggcgtag ttgcaagcct ggtcatgatg ctttcaggtg
     7501 tattgatggt attggtttcg ccgctggtgg ccaggatgat gttctgaaga aattccccga
     7561 tcaagcgacc ggggaagggg agagattaat gcgcgcggcc ctgctcaacg ccaaagccag
     7621 tctgggagcg gataaactgc gcacggaaca gttcacgctc gcgcgcacct tccgctgaat
     7681 tatccgttgc cgagaagaac cagataccga ggaatgccac gctgatagag aacagcgccg
     7741 ggtattcata cgggaagatg gctttttcgt gaccgaggat ctgtacccaa atcgtcgggc
     7801 cgaggatcat cagcaccacc gcagtaatca gtcctaacca gccgcccagc attgcgccac
     7861 gggtagtcag tttcgaccag tacatggaaa gcagaatgat cgggaagtta cagctcgccg
     7921 cgatggcaaa cgccagcccc accataaagg cgatgttctg attctcaaac agcacgccga
     7981 ggataatggc aatcacaccg aggatcagta cggtgatttt cgatacccgc agctcttcac
     8041 gttcggtcgc accttttttg aagacgttag cgtacaggtc gtgagacacc gccgatgcgc
     8101 ccgccagcgt cagacccgca accaccgcaa gaatggtggc gaaagcaacc gctgaaataa
     8161 agccgaggaa caggttgccg cccaccgcat tggccaggtg aactgctgcc atgttgttac
     8221 caccaatcag atggcccgcc gcgtctttat attccggatt cgcaccgacc agcatgatcg
     8281 cgccaaagcc gataataaag gtcaggatgt agaagtagcc cataaatccg gtggcgtaga
     8341 acacgctctt acgtgcttcg cgggcatcgc tgaccgtaaa gaagcgcatc agaatatgcg
     8401 gcaagcccgc cgtaccaaac atcagtccta gccccaaaga gagcgcggag ataggatctt
     8461 tcaccagccc gcccggcttc atgatgtcga cacctttcgg gtgtaccgcc atcgcttcgc
     8521 tgaacagatt gttgaagcta aagccgacgt gtttcatcac cataaaggcc ataaagctgg
     8581 caccgaacag caacagcacg gctttaataa tttgtaccca ggtggtcgcc agcatgccgc
     8641 cgaacaggac gtacatcatc atcagcacgc cgaccagcac taccgcaatg tgatagttaa
     8701 ggccaaacag cagctcgatc agtttacctg cgcccaccat ctgggcgata aggtaaagcg
     8761 ccaccaccac cagagaacca caggccgaaa gaatacgaat cggcccctgt ttcagacggt
     8821 aagaggccac atcggcaaag gtgtagcgac cgaggttacg cagacgttcg gcaatcagga
     8881 acaaaatgat cggccagcct accaggaagc ctagcgagta aattaagccg tcatagccgg
     8941 aggtaaacac cagcgcggag atccccaaga atgaggcggc ggacatatag tccccggcaa
     9001 tcgccagccc gttctgaaaa ccggtgatat tgccgcctgc ggtgtagtag tcattacgcg
     9061 aacgtacgcg ttttgacgcc cagtaggtaa tgccgagcgt aaacacgacg aaaatcagga
     9121 acataataat cgcctgccag ttcgttggct ggcgctctac ggccccgcta atagcatccg
     9181 cggcgttagc tgcgaaaggg agtgtggcgg caagcgccgt cagaactctt ttcatgatgc
     9241 ttgtacctca tgcaggactt cgttattaag acggtcgaat tcgccgttcg cccgccagat
     9301 gtagatacca gtgagaacaa aggagatcac aattaccccg acaccgactg gaataccgcg
     9361 tgtgacgctg gtgttcggat tcagcggggt gcccagccag ccgggcgcga aggcgatcag
     9421 taaaataaag ccgatataaa ctgccagcat aataatcgac aggatggtgg caaaccgttg
     9481 ccgtttttcg actaactccc tgaaatgcgc attgtcttct atccgctgat aaatcgtacc
     9541 attcatcaca gattctccag aggtaatgta gggattgttt taattcccgc tcccttatgg
     9601 gagaaggtta acgcttgggt aacccttgcc gaatgtaggc cggataaggc gttatacgcc
     9661 gcatccggca atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg
     9721 ccgaatgtag gccggataag gcgttcacgc cgcatccggc aaccagcgcc tgatgcgccg
     9781 ctgtcgcgtc ttatcaggcc tacaaaccgt taccgactcg catccggcaa ttgtgggtta
     9841 cgatggcatc gcgatggcct gcttctcttc aagcagcttc tcgactacgc caggatcggc
     9901 aagcgtcgag gtatcgccca ggttgctggt atcgcccgcc gcaattttgc gcagaatacg
     9961 gcgcataatt ttgccggagc gggttttagg cagggagtcg gtccagtgca gcacgtctgg
    10021 cgtcgccagc gggccaatct ctttacgcac ccagttgcgg acttctgcgt acagttctgg
    10081 tgacggttcc tccccgtgat taagcgtgac gtaggcgtag atcgcctgac ctttaatatt
    10141 gtgcggaata ccgaccacag cggcttcggc aattttcgga tgcgccacca gcgccgactc
    10201 aatctctgcc gtccccaggc ggtgaccgga gacgttcagc acgtcgtcca cgcgcccggt
    10261 gatccagtaa tagccatctt catcgcgacg cgcgccgtcg ccgctgaaat acatattttt
    10321 gaaggtggag aagtaggtct gctcaaaacg ttcgtgatcg ccaaacagcg tacgcgcctg
    10381 acccggccag gagtcggtga tcaccagact gccttcggtg gccccctcca gcgggttacc
    10441 ttcgttatcg accagcgccg gttgcacgcc gaagaacgga cgtgttgccg aaccggcttt
    10501 cagctcggta gtgccaggca gtggcgtgat catgaaaccg ccagtttcgg tctgccacca
    10561 ggtgtcgacc accgggcatt tctcgttgcc gatcttcttc cagtaccact cccacgcttc
    10621 cgggttaatt ggctcgccca cggaaccgag aatgcgcagc gacgaacggt cggtgccttc
    10681 gatcgcttta tcgccttccg ccatcagcgc gcgaatcgcc gtgggcgcgg tatagagaat
    10741 attgacctga tgcttgtcca ccacctgcgc catacgggca ggcgtcggcc agttcggtac
    10801 gccttcaaac atcagcgtgg tcgcgccgca ggccagcggg ccatacagca aataactgtg
    10861 tccggtaacc cagcccacat cggcggtgca ccagtagata tcgcccggat gataatcaaa
    10921 gacatattta aaggtcagcg ccgcgtacac cagataaccg ccggtagtgt gcagcacgcc
    10981 ttttggctta ccggtagaac cggaggtata gagaataaac agcggatctt cggcgttcat
    11041 ctcttccgcc tggtgctgag cgctggcttg ctcaaccagg tcgtgccacc acaggtcgcg
    11101 cccttcctgc cagtcaattt ttccgccagt acgcttcagt accaccacat gctctacgct
    11161 ggtgacgttc gggtttttca gcgcgtcatc aacgtttttc ttcagcggaa tactacgtcc
    11221 ggcacgcaca ccttcgtcgg aagtgatcac cagtcgtgaa ctggagtcga taatgcgtcc
    11281 ggcaacggct tccggcgaga agccgccgaa aatcaccgaa tgtaccgcgc caatgcgggc
    11341 acaggccagc atcgcgaccg cggcttccgg taccatcggc atataaatcg caactacgtc
    11401 acctttttta atgcccagtt cgagcagggt attggcgaag cggcagacgt cgcggtgcag
    11461 ctctttatag ctaatatgtt tgctctggct ggcgtcgtcg ccttcccaga tgatggcggt
    11521 acgatcgccg ttttcttgca gatggcggtc aaggcagttt gccgccagat tcagcgtgcc
    11581 gtcctcgtac catttaatgg acacattacc gggggcaaag gaggtgtttt tcaccttctg
    11641 gtacggtgtg atccagtcga gaatttttcc ctgttcgccc cagaaggtat ctggtgcgtt
    11701 aatagattgt tgatacatcg cctcgtactg ctgagggttt atcaggcaac ggtctgcgat
    11761 gttggcagga atggtgtgtt tgtgaatttg actcatgctt ttgttctcct tgtaagatgt
    11821 taataatatg tggcataagc gttaaatgta ggggtattgg cagttttgtt tagtatttgg
    11881 gcgacagatc acgcaaaagt agaattgtgc aaataaacgg cagggtaatt tttgaaggtc
    11941 agcaacaaaa gttgattaat tctttgagga acatgcagtt atgcatgctg ttgaaaaaga
    12001 ggaagatact gactaactct aaagtggtat tttacatgca cttacaattg attaaagaca
    12061 acattcacag tgtggttatt tgttacacat aggggcgagc aatgtcatga cagtgtaggt
    12121 gcggttactg tcgtgaaaaa caataaaaac cgccattgca acaatggcgc aattcggatg
    12181 aagccctatg acaaggataa aaataaacgc acgccgtatc ttcagcttat tgattccttt
    12241 tttctttttc acttctgttc acgctgaaca aacggctgct cccgcaacac ctgtaactgt
    12301 ggaagcgaag aatgaaacct ttgccccgca gcatcccgat caatatctct cctggaaagc
    12361 tacctcggaa cagtcagagc gtgttgacgc cctggcggaa gatccacggc tggtgatcct
    12421 gtgggcaggg tatcccttct cgcgcgatta caacaagccg cgtggacatg cctttgctgt
    12481 gaccgatgtg cgtgaaaccc tgcgtaccgg tgcgccgaaa aacgctgaag atggtccgct
    12541 accgatggcg tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc agaaagacgg
    12601 cgaagatggc tacttccacg gaaaatgggc gcgcggcggc ccggaaatcg tcaacaactt
    12661 aggttgtgcc gattgccata acaccgcctc accagagttc gccaaaggca aaccggagtt
    12721 aaccctttcc cgtccgtatg cggctcgcgc gatggaagcc attggtaaac cttttgagaa
    12781 agccggacgt ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg agtattactt
    12841 cgacggcaaa aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag tcgaaaatat
    12901 ggagcagtat tacgacaaaa ttgccttctc tgactggact aactccctgt cgaaaacgcc
    12961 aatgctgaaa gcgcagcacc cggaatatga aacctggaca gcgggcattc acggcaaaaa
    13021 caacgtgacc tgtatcgatt gccatatgcc aaaagtgcag aacgccgaag gcaaactcta
    13081 caccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg cgaactgcca
    13141 tacccaggac aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcagt cgattaacga
    13201 cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga aagcggcgct
    13261 ggatgcaggc gcaacggaag ccgaaatgaa gccgattcag gacgatatcc gtcatgccca
    13321 gtggcgttgg gatttggcga tcgcttccca cggcattcat atgcacgcac cggaagaagg
    13381 tctgaggatg ctcggtacgg cgatggataa agcggcggat gcacgcacca aactggcgcg
    13441 cctgctggcg accaaaggca ttacccatga aatccagatc ccggatatct caaccaaaga
    13501 gaaagcccag caggccattg gcctgaacat ggagcaaatc aaggccgaga agcaggactt
    13561 catcaaaacg gtgatcccgc agtgggaaga gcaggcacgt aaaaacggtc tgttaagcca
    13621 ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat gagcgtatta
    13681 cgttcgttat taactgccgg ggtgctggcg tcgggcctgt tgtggagcct gaacggaatt
    13741 accgctaccc ctgcggcgca ggcatctgat gatcgctacg aagttaccca gcagcgtaac
    13801 ccggatgccg cctgtctgga ctgtcataag ccagataccg aaggtatgca tggcaaacat
    13861 gcttctgtta tcaacccgaa taacaaactg ccggtcacct gcaccaactg ccacggtcag
    13921 ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga gccgatgtac
    13981 aaggttggag agcagaacag cgtctgtatg tcctgccatc tgccggaaca gttgcaaaaa
    14041 gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg ccattccctg
    14101 catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa gatttgcgtc
    14161 gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt tccgttgctt
    14221 aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggc gtgctggcag
    14281 ccgtcagcgg caccgcagga cgcgtggtgg cgaaaacgtt gaatatcaat ggggtgcgtt
    14341 acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg gatgcttgtc
    14401 gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt agcgagccgc
    14461 agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag cactgcgatc
    14521 atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgcgacgct gccagtggga
    14581 ttgtcgatgt taatccggat ctctgtgtcg gttgtcagta ctgcatcgct gcctgtccgt
    14641 accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat ttctgccgta
    14701 agacccattt gcaggccggt aagctgcccg cgtgcgttga ggcttgcccg acgaaggcgc
    14761 tgacgtttgg caatctggac gatcccaaca gtgagatttc gcaactgctg cgccagaagc
    14821 ccacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc gtaccgttta
    14881 aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat cgctggtgtg
    14941 ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc tggtgacgct
    15001 ggccgtgctg ttacgtcgct tctacccgca ggcaggtggt gcagacagta cgttgttgcg
    15061 caccacgctg attgtcgggc ctggcgcggt gatcctcggt ctgttgatcc tcgtcttcca
    15121 cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta cctcggtgat
    15181 gtcgatgggg gtgatgctgt ttcagctcta catggtggtg ctggtgctgt ggctggcgaa
    15241 aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc tggggatcgt
    15301 gcaaaaggtc ttgagcctgc tgacgcccgt tcatcgcgga ctggaaacat tgatgctggt
    15361 gctggcggtg ttgttggggg cttataccgg ctttctgctg tcggcgctga aatcgtatcc
    15421 gttcctcaat aacccgatcc tgccggtgct gttcctcttc tccggcatct cgtctggtgc
    15481 ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt ccacggaagc
    15541 gcactttgta caccgtatgg aaatccccgt ggtatggggt gaaatcttcc tgctggtggc
    15601 gttttttgtc ggtctggcgc tgggcgatga cggtaaagtg cgtgcgctgg tggcggcatt
    15661 gggcggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg ggctgattgt
    15721 gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctgccg tgctggcggc
    15781 atgtggggcc agcctggtcg gcgtgttgat gctgcgcttt ttcattctct acgccgggca
    15841 gttaacggtg gcgtaagtca gaacagaggt ggtttctgga cgtattcctt cctgaagtcg
    15901 gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg acggccttcg
    15961 cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc attctggcgc
    16021 agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc atcagcgatt
    16081 tctcggtcat ttatgtcgcc caacatagct atagcctgct gtcgtgggaa ctcaaactgg
    16141 cggcggtgtg gggcggtcat gaaggttcgc tgctgctttg ggtgctgctg ctttccgcct
    16201 ggagcgcgct gtttgcctgg cattatcgac agcaaaccga tccgctattt ccgctgacgc
    16261 tagccgtttt atctctcatg ctcgccacac tgctactgtt tgtggtgctg tggtccgatc
    16321 ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga tctcaaccca atgctgcaac
    16381 atcccggtct tatctttcat ccaccgctgc tttatctcgg ctacggcggt ttgatggtgg
    16441 cggcgagcgt ggcgctggcg agtttactgc gcggcgagtt tgatggtacc tgcgcccgaa
    16501 tttgctggcg ttgggcacta cctggctggg gcgcattaac agcggggatc atcctcggtt
    16561 cctggtgggc ctattgcgaa ctgggctggg gcggctggtg gttctgggat ccggtggaaa
    16621 acgcgtcgtt attaccctgg ctttctgcca cagcgctgct gcacagttta tccctgacac
    16681 gccagcgggg gattttccgc cactggtcgc tgttgctggc gatagtaact ctgatgctgt
    16741 cgctgctggg caccttaatt gtccgttctg gcattctggt ttcggttcat gcgttcgcgc
    16801 tggataatgt ccgcgccgtg ccgttgttca gcctgtttgc actgattagc cttgcgtctc
    16861 tggctctgta tggctggcga gcgcgggacg gtggcccggc ggtgcgtttt tcggggttat
    16921 cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta ctgatcgtgc
    16981 tggtgggaac gctttatccg atgatttacg gcctgctggg ctggggacgc ctctccgttg
    17041 gcgcgccgta ttttaaccgc gcgacgttac cgtttgctct gttgatgctg gtggtgattg
    17101 tgctggcgac gtttgtctct ggcaaacgtg ctcagcttcc ggcgctgctg gcgcatgcgg
    17161 gcgtgctgtt atttgccgcg gggattgtgg tttccagcgt cagccgtcag gagatcagcc
    17221 tgaatttaca gccgggtcag caggtgacgc tggcaggata caccttccgt tttgagcgcc
    17281 tcgatctgca agccaaaggc aattacacca gcgaaaaagc gatagtggca ctgtttgacc
    17341 atcagcaacg cattggtgaa ttaacgccgg agcggcgctt ttatgaggca cgtcgtcagc
    17401 aaatgatgga accgtcaatt cgctggaatg gcatccatga ctggtatgcg gtcatgggcg
    17461 agaaaaccgg agcggatcgt tacgcttttc gtttgtatgt acaaagcggt gtgcgctgga
    17521 tctggggggg aggattgttg atgatttcgg gcgcattgtt aagcggatgg cggggaagga
    17581 agcgcgatga ataaagggct tctcacgcta ctgctgttat ttacctgttt tgcgcgcgct
    17641 caggttgtag acacctggca attcgccaat ccgcaacaac aggaacaggc gttaaatatt
    17701 gccagccagt tacgttgtcc gcagtgccag aatcagaact tactggaatc caatgcgccg
    17761 gtggcagtca gtatgcgcca tcaggtttac accatggtgg aagaggggaa aagtgaagtc
    17821 gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa cccaccgtta
    17881 acgggtcaga cgctggtgtt gtgggcgctg ccagtagtgt tgttactgct gatggcgctg
    17941 atcctctggc gagtgagggc gaagcgatga aacagcccca aataccggtg aaaatgctga
    18001 caacactcac gatttttatg gtatttctct gtgtcggcag ttatctgtta agtccaaaat
    18061 ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc gctgcatcag tttgccagcc
    18121 agcaaacccc ggaagcgcag cttcaggcat tgcaggataa aatccgtgct aatccacaaa
    18181 acagcgaaca gtgggcgtta ctgggcgagt attatctgtg gcaaaacgat tacagcaatt
    18241 cgctgctggc gtaccgtcag gcgttgcaac tacgtggtga gaacgctgaa ctgtatgcgg
    18301 cgctggcgac ggtgctgtat taccaggcca gccaacatat gaccgtccag gctcgcgcaa
    18361 tgatcgacaa agccctcgcg ctggactcta acgaaatcac cgctctgatg ctgctggctt
    18421 ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga actatggcaa aaagtgatgg
    18481 atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat atggcgaaat
    18541 tgttgcagcg gagatcagat taatcatctg gtgatgaaga ttatgttatt gagctgcata
    18601 aaaaataatc gaatgaacat ataatcaaaa ataatcacca atcagtatta ttgcagatta
    18661 aacaaataaa aatccttcta taacaaatgg ttattcatta atcctgccat aaactcgcgt
    18721 tattatgcat taatgcagcg aaaagctctg ttgttaaagg gttgcgcaac ataccgcgca
    18781 aatgatactg atcataagcg ttaaaaaaat ctacaaacca acgcaacaca attcatgccc
    18841 tggcagtatg tcacgttctc gcgtttctga acggggaacg gcgctccatt gaggaagtta
    18901 ttctgatgaa aaatataaaa ttcagcctgg cctggcagat tctgtttgct atggtgctgg
    18961 gcattctcct gggaagctac ctgcactacc atagcgacag ccgcgactgg ctggtcgtca
    19021 atttgctctc tccggcgggt gatatcttca tccatctgat caaaatgatt gttgtgccga
    19081 ttgtgatctc cacactggtg gtgggtatcg cgggtgttgg tgatgccaaa cagctcgggc
    19141 gtattggtgc gaaaaccatt atctacttcg aggtgatcac caccgtcgcc atcattttgg
    19201 ggatcactct ggcgaacgtc ttccagcccg gtgccggggt ggatatgtcg caactggcga
    19261 ccgtcgatat ctcgaaatat cagagcacta cggaagcggt acaaagcagt tcccacggca
    19321 ttatgggcac gattttgtcg ctggtgccga cgaacattgt ggcgtcgatg gcgaaaggcg
    19381 aaatgctgcc gatcatcttc ttctcggtgc tgtttggtct ggggctttca tccctgcccg
    19441 cgacgcatcg tgaaccgctg gtgaccgttt tccgctccat ctctgaaacc atgtttaaag
    19501 tgactcacat ggtgatgcgt tatgcgccgg tgggtgtgtt tgcgctgatt gcggtgacgg
    19561 tggctaactt tggtttctcg tctctgtggc cactggcgaa actggtgctg ctggtgcatt
    19621 tcgccattct gttcttcgcg ctggtagtgc tgggaattgt ggcgcgcctg tgcgggttaa
    19681 gcgtctggat cctgattcgt attctgaaag atgagctgat tctggcgtac tccactgcca
    19741 gctctgaaag cgtgctgccg cgaattattg agaagatgga agcctacgga gcaccggcgt
    19801 cgatcaccag tttcgtggtg ccgaccggtt actcttttaa ccttgatggt tcgacgctgt
    19861 atcaaagtat tgccgctatt ttcatcgctc agctgtatgg cattgacctg tccatctggc
    19921 aggaaatcat tctggtgctg acgctgatgg tgacctcgaa agggattgct ggcgtgccgg
    19981 gcgtgtcgtt tgtggtgttg ctggcaacgc tgggtagcgt aggtattccg ctggaaggtc
    20041 tggcgtttat tgccggtgtt gaccgtatcc tcgatatggc gcgtactgcg ctgaacgtgg
    20101 tgggtaatgc gctggcggtg ctggtgattg ccaagtggga acacaaattt gaccgtaaga
    20161 aagcgctggc ttatgagcgt gaagtgctgg gcaaatttga taaaacggcg gatcagtaat
    20221 tgaagattgc cggggggata tccacccggc aatgtgtgga tgcctgactg agtaatagcc
    20281 tgatgtagcg tgatttttgt aggtcggata aggcgtttac gccgcatccg acatcaacgc
    20341 ctgatgcgac gcttgccgcg tcttatcagg cctacg
//
LOCUS       DAZKHK010000097        20202 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKHK010000097 DAZKHK010000000
VERSION     DAZKHK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.097"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1620)
                     /locus_tag="ACHOS5_003553"
     CDS             complement(<1..1620)
                     /locus_tag="ACHOS5_003553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9778311.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGGVDCLLISPERLANQDFLETVLYPVADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPILGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDESSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGST"
     gene            complement(1761..3101)
                     /gene="gntU"
                     /locus_tag="ACHOS5_003554"
     CDS             complement(1761..3101)
                     /gene="gntU"
                     /locus_tag="ACHOS5_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9778312.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3105..3632)
                     /gene="gntK"
                     /locus_tag="ACHOS5_003555"
     CDS             complement(3105..3632)
                     /gene="gntK"
                     /locus_tag="ACHOS5_003555"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9778313.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3771..4766)
                     /gene="gntR"
                     /locus_tag="ACHOS5_003556"
     CDS             complement(3771..4766)
                     /gene="gntR"
                     /locus_tag="ACHOS5_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP9778314.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(4859..5554)
                     /gene="yhhW"
                     /locus_tag="ACHOS5_003557"
     CDS             complement(4859..5554)
                     /gene="yhhW"
                     /locus_tag="ACHOS5_003557"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP9778315.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQCRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(5677..6714)
                     /gene="yhhX"
                     /locus_tag="ACHOS5_003558"
     CDS             complement(5677..6714)
                     /gene="yhhX"
                     /locus_tag="ACHOS5_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9778316.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVTLAR"
     gene            7049..7537
                     /gene="yhhY"
                     /locus_tag="ACHOS5_003559"
     CDS             7049..7537
                     /gene="yhhY"
                     /locus_tag="ACHOS5_003559"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9778317.1"
                     /translation="MSEIVIRHAETRDHEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIDMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(7598..9331)
                     /gene="ggt"
                     /locus_tag="ACHOS5_003560"
     CDS             complement(7598..9331)
                     /gene="ggt"
                     /locus_tag="ACHOS5_003560"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP9778318.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSNKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMSPPSSGGIHIVQILNILENFDMKKYGFGSADTMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            9451..9891
                     /gene="yhhA"
                     /locus_tag="ACHOS5_003561"
     CDS             9451..9891
                     /gene="yhhA"
                     /locus_tag="ACHOS5_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP9778319.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(9878..10621)
                     /gene="ugpQ"
                     /locus_tag="ACHOS5_003562"
     CDS             complement(9878..10621)
                     /gene="ugpQ"
                     /locus_tag="ACHOS5_003562"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9778320.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWHSKAFKGEPLPLLSQVAK
                     RCRKHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10618..11688)
                     /gene="ugpC"
                     /locus_tag="ACHOS5_003563"
     CDS             complement(10618..11688)
                     /gene="ugpC"
                     /locus_tag="ACHOS5_003563"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP9778321.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLLLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(11690..12535)
                     /gene="ugpE"
                     /locus_tag="ACHOS5_003564"
     CDS             complement(11690..12535)
                     /gene="ugpE"
                     /locus_tag="ACHOS5_003564"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP9778322.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(12532..13419)
                     /gene="ugpA"
                     /locus_tag="ACHOS5_003565"
     CDS             complement(12532..13419)
                     /gene="ugpA"
                     /locus_tag="ACHOS5_003565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP9778323.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(13517..14833)
                     /gene="ugpB"
                     /locus_tag="ACHOS5_003566"
     CDS             complement(13517..14833)
                     /gene="ugpB"
                     /locus_tag="ACHOS5_003566"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP9778324.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTSHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHITMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(15230..15943)
                     /gene="livF"
                     /locus_tag="ACHOS5_003567"
     CDS             complement(15230..15943)
                     /gene="livF"
                     /locus_tag="ACHOS5_003567"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP9778325.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(15945..16712)
                     /gene="livG"
                     /locus_tag="ACHOS5_003568"
     CDS             complement(15945..16712)
                     /gene="livG"
                     /locus_tag="ACHOS5_003568"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP9778326.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(16709..17986)
                     /gene="livM"
                     /locus_tag="ACHOS5_003569"
     CDS             complement(16709..17986)
                     /gene="livM"
                     /locus_tag="ACHOS5_003569"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP9778327.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLVRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(17983..18909)
                     /gene="livH"
                     /locus_tag="ACHOS5_003570"
     CDS             complement(17983..18909)
                     /gene="livH"
                     /locus_tag="ACHOS5_003570"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP9778328.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(18957..20066)
                     /gene="livK"
                     /locus_tag="ACHOS5_003571"
     CDS             complement(18957..20066)
                     /gene="livK"
                     /locus_tag="ACHOS5_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP9778329.1"
                     /translation="MKRNAKTIIAGMIALTISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         4917 a         5675 c         5017 g         4593 t
ORIGIN      
        1 cgtagatcct tgtaaacccg caggcaagtt ccctttaaat ccgtattgcg taaaagcccc
       61 agatgccacc tgtttattga gatttaatgg caggtcagcg tgtttaataa ataaatttgc
      121 cgcatggagt aagccgctat caatgtcggg cgacaacagc ggatgctgaa gacaactgct
      181 acatttacca caacgttcgg cacttgactc atcgagtgcg cggcgcagaa attgcatttt
      241 gcactctttg gtcgtgatat agctttcaac gtctgccaac tcactttttc tctgattcat
      301 caaatggtta attcgttcat gatcaggaga aaaactgacg atagttctgc gccataattt
      361 ctcggtatac accacaggcg atggattttc agctaccaat aatttcagtg ctttttctat
      421 ttgcccataa cgaagattcg tccgttgttc aatgcctcgt agggtaagac cgtcattctc
      481 gctaagtacg ttgagtattt catgaatttg cgcctccgca ggaaaggcgc tttcacggaa
      541 gaattgatga atagcgcggt cttcaccacc acaaagcaat atgccaactg cggaatctat
      601 agcacgcccg gcacgcccca cctgttggta gtaaccgaca atagaccccg gcatctgata
      661 atgaatgacg aaacctaaat caggtttatc aaatcccata cctaatgccg tcgtcgcgac
      721 cagcactttg attttatttg ccagcagtgc ctgctccaga tactccctgg cagtattaga
      781 atcttccgca ccttcacaag tcacgccgct gtaataagca aatgcactga taccattttt
      841 ccttaaccac tcggcgacaa gttcagcatc gcgagtcgtc agggtataaa caattcccga
      901 tttggaaaac tgagggataa ccgttgctaa ccatgccaga cgggatgact gttctcctaa
      961 gactaaggca tcaagcgcca aactttcgcg agccagcgtt ccacgctgaa tcacaatgtc
     1021 acccaattgc tgacggatat cctcaacgac acggttattc gctgtcgcgg ttgtacccag
     1081 aataggggta ttcgcaggca gttggcgcaa aatatctaat atacgtcggt aatccgggcg
     1141 gaaatcgtgg ccccaatcag agatgcagtg cgcctcatcg accaccagta aaccgatgcg
     1201 gtcagcgact ggatataaaa ccgtttcaag aaaatcctga ttcgccaacc gttcaggtga
     1261 aatcagcaga caatctacgc caccttgcag caacttatcg ctaatgcgct gccattcttc
     1321 cctgtttgta gaattcaacg tttcagccgt aatacctaac cgttcagcgg cggcaacctg
     1381 gttacgcatc agggcaagca aaggagagat gataattgtc gggccagcgc cgcgatcgcg
     1441 gaagatttta ctggcgatga agtacacggc acttttcccc cagccggtgc gctgtacaac
     1501 cagtagtttt cgccgctgat taaccaccgc atctatagct tcccattgcc cttccctgaa
     1561 agtagcgctg gtgttactta acatccgctg taacagcaat tcagctccgt gtttctccat
     1621 catcttctcc ctcgtccgtt aacgcagtga ttagaacgga taatgttccg gtctttttgc
     1681 gagagagatc aaaaaaatga agacaggaga aaagagaaat gaattactgc gagcaagctc
     1741 gcagtaattt gtcgcagtaa ttaactcaac agctggaacg caatcatccc aacgatggca
     1801 ccgacagtgc cgaggatggt ttccatcatg gtccaggttt taagcgtttc ggcttcggtc
     1861 gcgccggtaa atttaccgaa caaccagaaa cccgcgtcgt taacgtggct gacaacaatc
     1921 gaaccaccgg cgatacaaat cgacagcgcc gccatttgcg caccggagta gttcagttgt
     1981 tcaatgaccg gcattaccaa tcccaccgcc gttaaacagg caacggtggc agaaccctga
     2041 atgatgcgca ctgcagctgc cagcacgaag caggtgatgg caatcggcag gcccatgccg
     2101 gttaacgctt cgcccagtgc cggaccgaca ccagagtcaa ccagcacctg tttgaacacg
     2161 ccgcccgcac caatcaccag cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc
     2221 tccatcactt tgtcttttgg cataccctga cgcattgcca gaccgtaaat cgccaccagg
     2281 caagcaacca gaatcgcggt aaacggatga ccaataaact cgaaccattc gtaagcagta
     2341 gaaccttctg gcacaaaacg cgcggcaatg gttttcagcc ccaccagcac cagtggcaac
     2401 aggatcagcg acaggctgaa tccgaaggac ggcattttgc cttcgccgag atgcggttcg
     2461 ctgatgtcgt caggaatatg caactccacg tagcggctga tgaaattacc ccacagcggc
     2521 ccggcaataa tcattcccgg aattgccgcg cacaggccaa tcaggatcat ccagccaaaa
     2581 tcggcattca tctgcgatgc cagcagcatt ggcgctggcc caggcaccag gaacgcagcc
     2641 gctgccgcca cgccggcgaa taatgggatt accagcttca ccaggttcgt gccggtgtgg
     2701 cgcgccattg agaaagcaac gctaatcagc agaacaatcg ccacttcaaa gaacagcggt
     2761 agcgcacaaa ccagccccgc aaggccgatg gcataatgca cgcggctgtg accgaaggat
     2821 ttgagcattt tgacggcaat ctgatcgacc gcgccggttt catgtaagat cttaccaaac
     2881 atggctccca gggcgacaac caccgccagg aagccgaggg tgcctcccat ccctttttcc
     2941 atcgtcgctg cgattttatc gagcggcata ccagaaaaaa ggccagcccc catggacacc
     3001 accattaaag ccaggaaagc gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa
     3061 acagacccta ctgctgttaa aacaagcgtt aatgtagtca ctacttattt gcctttttta
     3121 ataacctcaa tggtgcttgc cacaacacct tccagcggtt gatcaatatc caccaccagt
     3181 acatcggttt cgtccgcacc cggctcctgc agcgtttcaa actgcgtcac cagcatttgg
     3241 gttttaaaga aatggccttt gcgcgctttc aggcggcttt caatcacatc aaaatcgcct
     3301 ttcaggtaga tgaaagagag attaggatta ccttcacgca gcaagtcgcg atagtgtttt
     3361 ttcagtgcag aacagacgat cagcgatact ttattggtgc gctgcatagc aaacgcggcg
     3421 tcgttcagcg cctgcaacca cggtttgcga tcgtcgtcat tcagcggttc gccagacgcc
     3481 attttttcga tattgcagcg cggatggagg aaatcgccat caagaaacgc ggcatgaagt
     3541 tgatgcgcca cttcactggc gaccgcagat ttgccgctgc ccgatacgcc catcaagacg
     3601 taaatgtggt gatcatggtt agtcgtgctc aaagtggtgc ccccacaata caagaattaa
     3661 aaatgttacg ggtaactgtt atcggtaaca ttgtccagcc ggacaatatc agaagcaata
     3721 tccatccgtg ccttaagtgt ataagtgtga gctacttcaa atttgtaggc ttaaatagat
     3781 ccgcccggtg acaaggtgaa acctaaatct aacattttcg gtgtcacaga ttcgccacga
     3841 atacgcgcca gcaggcgctc agcgccaatg ctgcccatcc gctcacgcgg cgtcagcaca
     3901 ctcgccagcc gtggctccat cacctgacca atgtcatgac cgtggaaacc ggcaatcgcc
     3961 atatcgtcag gaacttttaa ccccagacgc tgacattcaa acgccgcgcc gaccgccagg
     4021 tcatcattag tacagaacac gccatccagc tgcggatatt cccgccgcgc ctggcgaatc
     4081 agttcaatac cggaagagta agaagaagat tgctcaacca tcacgctata cggcaccagg
     4141 cctgcatcca gcatcgcctg ttcgtatccc ttctgtttga tgatagtacg ttcgtcgagg
     4201 cgtgcgccga gataggcaat gtgacgatgc ccgcgagcaa taatggcggt ggtcatctgg
     4261 cgtgccgctt caaagttatc aaaaccgacg gcgatatcga ggcacggcga cttactgtcc
     4321 atcaactcca ccaccggaat ccccgccact tcaatcatct ttaaggtacg cggcgtgtgg
     4381 gtacgttcgg tgaggatcag gccgtcgata ttccatgaga gcattgattc gaggcgttct
     4441 tgctccattt ccggtttata accgtagtgc gccagcatgg tttgataacc gtgcgcgtcg
     4501 gtgacgcttt cgattccgcg taatacttcc gcgaaaacct ggttggtgag agaaggtaac
     4561 aggacgccaa tcgcccggct ggtggcgtta gagagtatat cgggcgcgcg attgggaata
     4621 taacccagtt catcaagagc ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc
     4681 gggttgcgta aaaaacggct gaccgtcatt ttggtcacgc ctacacggtc agccacatcc
     4741 tgaagtacgg gtcttttctt tttcatcgtc ctgaaggtac aaaagagata gattctctta
     4801 gtttaacacg gacgcatcag aaccttcccg attcctcagg aaggtttgtg agataaattt
     4861 agactggcgg taaatcaaac agcaggattt cactgtcgct atcagcatgg atggaaatcg
     4921 cctgctcatc ccagattgcc agaccatcgc tggtcgaggc tttcacgccg ttaatggtga
     4981 cattgccttt caccacctgg atccagacgc ggcgttcagc ggcaatctga tgcaccgact
     5041 gctcatcttt caccagcgcc caacgataca gttccatatc ctgatgcact ttcaatgaac
     5101 catcacgcgc atccggcgaa agcaccagtt gtttgccctg cacggcatcg aagcgacact
     5161 gttcataacg cggcgtaatg ccgttttctt caggcatgat ccagatctga tacagatgca
     5221 gacgctcggt gctgcttggg ttgtactctg agtgacgaat acccgtaccc gcactcataa
     5281 tctggaactc gcccgccgga acctgctctt tattgcccat gctgtcctga tgctcaacag
     5341 taccttccag cacgtaggtc aaaatttcca tatctttatg cgggtgagtg ccgaagccct
     5401 gccctgcttc aatcacgtcg tcgttaatca cgcgcagcgc agagaagccc ataaaattcg
     5461 gatcgtaata gttggcaaaa gagaaagtat gccaggagtc cagccagcca tgatttgcat
     5521 gaccgcgttc atttgctttg cgtaagtaga tcatttgtat tcgccccctg aatgatttcg
     5581 atgggcttag tgtggacccg atccgcctgg gatgatagag ggtgaaaatt gacccctctg
     5641 ttcaaaaaaa ttgaactatt cgaggggcca tcaaacttac ctcgcgagag tcactgcgga
     5701 gggagaagct tgctcaaaac cgcgttcaag gatttccaga ttcgtaagaa cttcagattc
     5761 cttgacgtaa tttggcgcac cgttggtgat ggtttgatac aacgcatcat aaacgcgccc
     5821 ataatcgccc atctctggct tcatctcttc ccttaccgtc acgccctcgt cattgacata
     5881 ctccagcaca ccgaccgaat catccgctgc gaatcctggt tcgcccggca taatattagc
     5941 cttcaggctg gtttcctgct ggtcgatacc gtatttaata aacgaacctt tcttaccgtg
     6001 aacgataaat ttcggataat cgattttcac cagatggctg gttttgacga tggcttttag
     6061 atcgccataa aacaactgcg cttcaaaggt gtcgtccgga ttggctttat tacgcaggct
     6121 gcggatgtca taagcgacgt gatccgggcg accgaacaga gaaataatct ggtccatcgt
     6181 atgcacacca aggccataga acgcgccatc ctgcggtagc cccggtttgg tttctgccac
     6241 cgggcggtaa tagtcaaaat ggctttccac ttcgacaatc tctcccagct tgccgctttc
     6301 aatcgctttt ttcgctgtca ggaagcagga atcaaagcga cgattctgat acggcgtaac
     6361 gatcagccct ttgcttttcg ccagtgcaaa cagctctttc gcctgcgcaa ttgtcggagt
     6421 gaacggtttt tcgaccagca catttttccc ggcttccagc gcgcgcttcg cgtactcgaa
     6481 gtggctgtcc gcgtgggtgc agacgacaac cagcttaaca tcgggatcgt ttagcacttc
     6541 gtcgagatcg ctggtgaaat ggatatggga ataaatgggg gcctgttctt ccggcttcgc
     6601 atggcggcga aaaatatgcg cgacatgcca gctatccttg cggttaagta catacggcag
     6661 atggtaacgg gtggtgcttt tgccgaagcc aataaaggcg cagttaatga ccatgatttc
     6721 gtccttttta aggtggttat ttacacctta gcgcaaagcg gacgtggttc ctactggagt
     6781 gcgcataacg aacacaagca cttcccgagg ataaattgag aacgaaaggt caaaaaaaaa
     6841 agccagcacc cggctggcta agtaatactg gaagcaatgt gagcaatgtc gtgctttcag
     6901 gttctccgcg agggtcttcc tgatcgcgag acaataataa tcattctcat tcgcacttgt
     6961 ccaacacttt ttgcaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag
     7021 ggacatttac cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac
     7081 acgcgatcac gaagccatca ggcagattca cgcccagccg gaggtgtatt acaacacgct
     7141 acaggtgcct catccttcag aacaaatgtg gctggaacga ctcaccgctc gccctggcat
     7201 caagcagtta gtcgcctgta ttgatggaat cgtcgtaggc catctcacca ttgacgtgca
     7261 acaacgccca cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgctggaa
     7321 gaaccgcggc gtcgccagcg ccctgatgcg cgaaatgatt gatatgtgcg acaactggtt
     7381 gcgggtagat cgcatcgagc taacggtgtt cgtcgataac gcgccagcaa ttaaggtcta
     7441 taaaaaattc ggctttgaga ttgaagggac tggcaaaaag tacgcattac gtaatggtga
     7501 atatgtcgat gcgtattata tggcgcgggt gaaatagaga atttgcaatc tacgtttgtc
     7561 atttaagccc tcacctaccg ggagagggct taagatatta ataccccgcc gttaaatcat
     7621 ccaccgagcg cgggtcggat gcaccgtaca gctcaccgtc cggcccaacc ataatgcttt
     7681 gtgtactgcc catcgcctct ttcagcgcca ctttctgacc ttttgcttcc agcagcttga
     7741 tcgtatccgg gctaaaccct ttttcgacac gcagctcgtc cggcaaccac tgatggtgga
     7801 aacgcggcgc attggtcgct tcggcgacgt tcattccata atcgatgcta ttcaccacca
     7861 tttgcagcac agtggtgata atccggctac cgcctgggct tccggtgacc agccaggttt
     7921 taccatcctt gaccacaatg gttggtgaca tcgacgacag cgggcgttta ttcggcccga
     7981 cggcgttggc atcgccgccc accagcccgt aaacgttcgg cacgcccggt ttggcggaga
     8041 aatcatccat ttggttatta agcagaatac cgctctcgcc cgcgacaatg cccgtaccga
     8101 aggtggtgtt cagcgtatag gtcaccgcca ccgcgttacc atctttatcc accactgagt
     8161 aatgggtagt ttgattactc tcataaggcg caagcttgcc ggggcgaatt tcgctggacg
     8221 gcttcgcttt gttgatatca atttgatcgg caatagattt ggcataggct ttattggtca
     8281 gcgcctgcca cggtactttg acaaaatccg ggtcgccaag atattccgag cggtcggcgt
     8341 aggcgtattt ctcagcttct gccatgattt gcatcgtatc ggcgctgcca aagccgtatt
     8401 tcttcatatc gaagttttcc agaatattga ggatttgtac gatatggatc ccgccggaag
     8461 atggcggtga catggagtaa acctgatacc cgcgataatc gccgcttatc ggtgtgcgct
     8521 cgaccgcttt ataggccgct aaatcttctt tcgtgatcag gccgccgttt ttctgcatct
     8581 cctgagcgat ctgctccgct attgtgcctt tgtagaattc gtccgggcca ttttcggcaa
     8641 tcatctccag gctctttgcc aggttcgcct gcaccagctt gtcgcccttt ttcagcggtt
     8701 cgccctcttt ccagaagata gctttgctgt tttcgtggtt cggcagcact tcactgccgt
     8761 aggttttgag atcgtcagcc agcgcgtcgt taacgataaa accatcgcgt gccagtttaa
     8821 acgccggttg caccacttta ttcaacggca tggtgccgta tttatccagc gccaacgaga
     8881 aacccgctac cgtaccgggt gtgccggaag ccagatgcga agtgagtgat ttgttgctgt
     8941 ccgggttgcc ctgatcatcg aggaacatat cgcgggtcgc tttggcgggt gccatttcgc
     9001 ggaaatcgat agccgtggta ttgccatttt tcgagcggat cagcataaaa ccaccgccgc
     9061 ccagattccc tgcctgcgga tgcgttactg ccagcgcgta gcccaccgcc acggcagcat
     9121 caacggcatt cccgccctcc ttgaggatat ccacccccac ctgagtggca gtagcatcta
     9181 cagacgccac cattccctgc ttagcgcgta ccggatggaa gacatcttcc tccacaccat
     9241 aagaaacagg tggcgcggct accgtactaa aacaacttcc tgtgagcaga gcagcaatgg
     9301 ccacccggcg taaaaacgtc ggttttatca tcgttattct ccagagatta aggggcaacc
     9361 ccagctaagc ctggtatata actctgaatt aatcatcgtt ttgccgggaa gcgagtaaac
     9421 ttaaagaata tcttcagagg aggatacgcg atgaaacgac ttctgctttt gacggcactc
     9481 ctgccgtttg tcggctttgc acagcccatt aatactctga acaaccctaa ccagccggga
     9541 tatcagatcc ccagccagca gcggatgcaa actcaaatgc agacgcagca aatccagcaa
     9601 aaagggatgc tgaaccagca actgaaaacg caaacgcagc tgcaacagca gcatttagaa
     9661 aaccagataa acaataattc tcagcgggtg ttgcaatcac agccggggga gcgaaatccc
     9721 gcccggcagc aaatgctgcc caataccaac ggcgggatgt taaacagcaa ccgtaatccg
     9781 gatagttcgt tgaatcagca gcatatgctg ccggagagga gaaacggcga catgctgaat
     9841 cagcccagca cgccgcagcc tgacattccg ttgaaaacta ttgggccgta aagttcggac
     9901 caatcacgtc aatcgcatcg gtacagatgc aatccacgcc ccagcgcagc aactctgccg
     9961 cgcgctgggg tttattgacg gtataaacca gaatccgtaa tccggcgtct ttcaactgca
    10021 tcactcgcgc tttatcgagc aacttatgat tgagatgaat agagacgcag cccagccgcg
    10081 cggtcaattc tcgccagtcg tcgcgccatt catcaagcaa taaaccacgc ggcagttccg
    10141 gtgctgcctg ttgcgcggct tctaaagcat caatctcaaa cgatgacagc agcggcggcg
    10201 tcataccggt ccacagttcg cgtgccgcca acgccaccgt tttgcccgtt aatggtccag
    10261 tgccagtggt gggtttgatt tcgatattcg ccatcatccc gtgtttgcga caacgtttcg
    10321 ccacctgcga aagcaacggc agcggctcac ctttaaacgc tttgctgtgc caactgcctg
    10381 catccacgcg cagtaaatcc tgccagttca gttcacccgc gacgccccag ccgttgctgg
    10441 tgcgttcaag attgtcgtca tggagcagga agatctcgcc atctttcgat aactttgcgt
    10501 caaattcgat catcttatga ccgtatcttg ccccgacgtc gattgccgcc agagtgtttt
    10561 ccggggccag cttaccgcca ccacgatggg cgacgatgcg gggataaggc cagttactca
    10621 tactcgttgt cctgtttcac catcaaaaag atgcagctga ttttccggca gatgcagcca
    10681 cagcgtgctg cctgccgtcg ggcgctcctg atgcgccagt cgcaccacca gcttctgttc
    10741 gccccagcgt ccgtgcgcca ggttatctgc gccgaggatc tccagcgtgt ccatcaccag
    10801 cggcacacca ccttctgcct gcgagcttag cgctatatgt tccgggcgaa tgccgagggt
    10861 cattttacgc ccggcatact gacggtagcc accgtttagc agcagcgtaa taccaccatc
    10921 cagttcgaaa tgcgtacctt cgttattcac acgccctgcc agcagattca tcgccggact
    10981 gccgataaaa ctcgccacaa acaggctggc gggcttttcg tacacttcaa ctggcgtgcc
    11041 aatctgttcg gcaacgccgc cgttcatcac cattactcgc tgggcgagcg tcatcgcctc
    11101 aacctgatcg tgggtaacgt agagtgaagt cgttttcagg cgacggtgca gctgttgcag
    11161 ttcaagacgc atctgcacac gcaacttggc atcgaggtta gagagcggct catcaaacag
    11221 gaacactgcc ggctcacgca caatcgcacg ccccatcgcc acacgctgac gctgaccgcc
    11281 agatagctca cgcgggcggc gcttaagcag gccgtccagc tccagaatgc gcgccgcttc
    11341 tttaacacgt tcggcaattt gttgcttgcc catgccgcga attttcagcc cccacgccat
    11401 gttctcctct acgctcatat gcggatagag cgcgtagttc tggaacacca tcgcaatccc
    11461 gcgatctttc ggctccattt cggtgacacg cttgcggtcg atccagatat cgcccgtcgt
    11521 tacccgctcc agtccggcaa ccattcgcaa taacgtcgat ttcccgcagc cagacggccc
    11581 gaccatcacg ataaattcgc catccgccac atcaagggtc agcggtttaa tgacctgggt
    11641 tttgccatcc cagcttttgg ttactgcctg taatttcagt cctgccatct tatttctcac
    11701 tatcgaccag gccgcgcacg aaggcacgct gcatcactaa aacaatcact actggtggga
    11761 tcagggttaa caacatcgcc gccatcaccg agttccattc cgttgtgcct tcgcctgtgg
    11821 cgatcatccc tttaatccct gccacggtgg tgccgagatc aacgtcggta ataatcaaca
    11881 acggccacaa atactggttc cagccgtaaa taaaggtaat cacaaacagc gccgccagat
    11941 tagttttgga cagcggaaaa acgatgtcgc aaaagaaacg cattggcgac gcgccgtcga
    12001 tccgcgcggc ttccaccagt tcatccggta gggtcataaa gaactggcga aacagaaacg
    12061 tggcggtcgc cgaggccatc agcggcagcg ttaaacctgc gtagctgtcg agcattttca
    12121 ggttggcgat gacttccacc gtcgggaaga tacgcacttc aaccggcaac atcagggtga
    12181 taaaaatcat ccagaagaag aggttacgta gcggaaaacg aaaccagaca atggcaaatg
    12241 ccgagagcat cgagacggta attttgccga gcgtaatgct gaacgccatc acaaagctgt
    12301 taagcaacat ccgccagaac ggcgcgctat tcgtgcctac cccgtttacc cagatgttgt
    12361 ggatgttttc cagcagatgt gtgccgggga ttagcgtcat cggtgcggca tagacttctt
    12421 gtttatccag cgtcgccgcg acaaacgcca cgtacagcgg gaagaggatc accgcgatcc
    12481 cgaggatcag catggtatgg ctgaatattg tcagccacgg acggttctca atcattggta
    12541 acgtaccttg ctttcaacat agcggaactg caccaccgtc agcacgatga cgaggaacat
    12601 caacaccacc gactgcgcgg cagacgaagc caaatccagt ccggtaaagc cttcacggta
    12661 aatcttataa atcagcgtcg tggtggcctg tactggcccg ccggacgtgg cggcgtcgat
    12721 caccgggaag gtgtcgaaga aggcatacac caggttcacc accagcagga agaaactcac
    12781 cggagcgata agcggcagcg caatcttaaa gaagcggcga atcgggccag ccccatcaat
    12841 ggctgcggct tcgatcaacg aacgggggat ggattgcagc gcggcataga agaacagaaa
    12901 gttgtagctg atttgcttcc acactgaggc aaacaccacc aggaacatcg cctgaccgct
    12961 gttttgcgcg tggttccagt cgtagccgaa ctcagcgaga aaatgggtga tcagcccgcg
    13021 accagggtta aacagaaaga tccacaatac ggcggcaacg gcgggagcca cggcatacgg
    13081 cagcagcatc aaagtttgat agaaacgact gccgcgcacg atgtactcca ccagcgctgc
    13141 gaagaacagc gataccagca aaccgctgac ggtgacaaag gtgctgaatt ttatcgtcgt
    13201 ccagaaggcg tcgagatagt agctgtcgtg aaacagcgtg acgaaattat ccaggccgac
    13261 aaactggctg gagaaaccaa acggatcgac gctttgtagc gagtaccaca acgcttcgcc
    13321 cgcaggccag ataaaaaaga taacggtgat gatgagctgc ggcgcgacca gcaaataagg
    13381 cagccagcgc gagcggaaca ccggacggga tgatgacatt acagttaatt cctgaactgt
    13441 gccggatgcg cttcgcttat ccggcctaca cgcgatgcca ggtcagataa gcgaaacgca
    13501 tccgacatta cactgattaa gacttcgtcg atttctcaaa gcggcgcagt aactggttcc
    13561 cacgctcaac ggcagtatcc agcgcctgct gtggtgtctt cttaccggtc cacacactct
    13621 ccagctcttc atccacaatc acgcggatct gcggcatgtt gcccagacgc aaacctttgg
    13681 tgaacggcaa cggtggcttg ttcagcatct gacgcgtggc aatatccgct cctgggtttt
    13741 tctcgtaaaa gccctgctca cgggtcaggt catacgccgc tttagtgatt ggcagatagc
    13801 cggttttctg atgccactcg gcagcgtttt ctggctttgc gaggaagtcg aggaacttcg
    13861 ccacgccggt gtaggtttct ttatctttac cctgcattac ccatagactg gctccgccga
    13921 taatggcgtt ttgcggcgcg tctttcgcat cggcgtcgta aggcatcatg cctacgccat
    13981 agttaaattt ggcgtactcg cgaatgttgg caagagaacc ggaagaggcg gtcgtcatcg
    14041 cgcaatcacc gttatagaac ttctcggtgg attcatcctt acgcccaacg tagctgaaat
    14101 cgcccttctt gttcatctct tcgagcatgg tgatgtgttt cacctgctcc ggcttgttga
    14161 actccagcac cgcgtctgtg ccgtcaaagc cgttgttttt gctggcaaac ggcagaccgt
    14221 tccaggcgct aaagttttcc agttggatcc atccctgcca gccgctggcg tagccgcact
    14281 tcataccgga ggctttcagt tttgcggcat agtccgccag atcctgccag gtttttggcg
    14341 gctgttccgg gtctaatcct gctttcttga aggcgtcttt gttgtaatag agaacgggag
    14401 tcgagctgtt gaacggctgg gagagtaagt ggctggtttt gctgtcggag taatagcctg
    14461 aaaccgtcgg cacaaactgc gactcatcga attgaatccc cgcctcttta aacacgtcat
    14521 acaccggttt aatggctttc gacgccatca tggtggcggt gccaacttca taaacctgca
    14581 aaatagccgg agcgttgccg gtacgaaatg cggcaatccc cgcgcttaaa ttctgttcgt
    14641 agttgccttt ataggtcggt acaattttgt aatccgggtt ttcggcgtta aaacgttggg
    14701 ccagagaatc cacctcttta cccagttccc cttccataga atgccagaac ggaatggtcg
    14761 tcactgcctg cgcatttgcc attaaagcca gtccgagcgc cagtgctgaa gctgtataac
    14821 gtaacggttt cattgattat ctctcttgtt gtaccgaatg cgcgaattca cgcgttttat
    14881 gctcgcggag taacatgaca tgctcgaatt acagaaaaat aactattttg ttacattttt
    14941 aagatagtaa ggtgtcagaa agatgacaag gcggtgacgg cgcgggcgag ggaaaatggg
    15001 agatggggca cggataagcg gaaaaataga aggtctgaat caaactctac agattactca
    15061 tcgtttcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaagatcg tgcaaattca
    15121 atatattgca actcacccag taggcctgat aagcgcagcg catcaggcaa ttttacattt
    15181 gtcacctgtc tcaaaggagt cttttgactc cttatcaatc aacgtgttat tacccgccta
    15241 aatacgcact tctcaccgct tcattcgcca gcagcgcgtc gccggtatcg gagagcacta
    15301 catgaccgtt ttccagcacg tagccgcgat ccgccagctt tagcgcctgg ttggcgttct
    15361 gctcgacaag gaagatggtc atcccctgct cgcgcagttg ttcgatggtg tcgaaaattt
    15421 gctggatgat aatcggtgcc agcccgagcg atggctcatc aagcaacaac aaacgcgggt
    15481 tgctcatcag cgcgcgacca atcgccagca tctgctgttc accgccagac atagtgcccg
    15541 ctcgctgaac acggcgctca tgcagacgcg gaaacagttc atagacccac tttatgcgct
    15601 cctggaactg gtcgcgttcg gcaaaaaagc cgcccatcgc caggttctct tccaccgtca
    15661 tccgcgagaa gacgcgacgc ccttccggga caatcgctac cgcttcgcgc atgattttcg
    15721 ccgtctgcca gtcggtaatg tctttatcat caaacacaat tcgcccgctg gtggcgcgcg
    15781 gatcgccgca taacgtgccg agcaaggtgg ttttccccgc cccgttcgcg ccaatcagcg
    15841 tgacaatttc gccctgattg atatgcaggc tcacctcatg cagcgcctgg attttgccgt
    15901 agtgggcgct gactttgtca aaggacaaca tgactttttc catcttatgc ctcacctaaa
    15961 taggcacgga tcacatccgg gttattacgg atctgctccg gtgtaccgtt tgccagcggc
    16021 gtcccctgat tgaccacgta aattctgtct gaaatcccca tcaccagctt catatcatgt
    16081 tcaatcaaca agatagtggt gttgtgatgg ttacgcagtt cggcaatcag ctcatccagc
    16141 tctttcgtct ctttcgggtt aagacctgct gccggttcgt cgagcattaa aatctccggc
    16201 tgcgtcacca tacagcgggc aatctccaga cgacgctggt caccataggc caggttactc
    16261 gcctgacggt tggcatgttc caacaaacca atgcgctcaa gccaggtagc ggcgcggtcg
    16321 agcgcttcgc tctgggcgcg acggaaagag ggggttttca acaggccgga gaacagccca
    16381 gttttcagtt gctgatgctg tgccaccagc aggttttcaa tcaccgtcat ttcacggaac
    16441 agacgtacat gctggaaggt gcgcaccacg cccatgcggg caatttgctg ccccggtaaa
    16501 ccttccaggt gctgatcgcg cagtaaaatg gtgccgccgg tgggtttata gaatccggtc
    16561 aggcagttga agaccgtggt ttttccggca ccgttagggc cgattaacga gacgatctcc
    16621 tgcgggtaca gttcaagatt gacgttgttc accgccagca ggccgccgaa gcgcatcatc
    16681 aggccgttaa cagataataa tggctgactc atgcctgctc tccttttgct gcgccgtttt
    16741 tcagcttgag ttgcgggcgc gtcatgggca gcaagccctg cggacgccag atcatcatca
    16801 gcaccatcaa accaccgagc attaacatgc tgtattcgtt gaaatcacgc atcaactcgc
    16861 gcgataccac cagcaaaaca gccgccagaa tcactgcaaa ttgcgagccc ataccgccga
    16921 gcaccactat cgccagcaca aacgccgatt cggcaaaagt gaaggattca gggctgacaa
    16981 agccctggcg cgcagcaaac aacgttccgg caaaaccggc aaacgcagcg ctgatggtaa
    17041 aggcagtcag cttgatacga cgcgggctta agcccagcga acgacaagcg atttcatctt
    17101 cacgcaacgc ttcccacgca cgccccagcg gcatccgcag caggcggtta atgacaaaca
    17161 ggcttaacac cactagcagc aacgccacca gatagaggaa aatgacgcga tcggagggat
    17221 cgtatttcag gccaaagaaa ttgctgaacg tgtcccagcc gccttcgcgg gcggtacggc
    17281 tgaactcgag tccgaagagc gtcggtttcg gaatctgact gataccgttc ggtccgccgg
    17341 taatttcggt gttattgagc agcaatatgc gcacaatttc gccgaacccg agggtaacga
    17401 tcgccagata gtcaccgcgc agacgcagca ccggaaaacc gagcaggaag cccgccgccg
    17461 ccgccattaa cccggcaatc ggcagacagg tccagaagcc caggccgtaa tagtgattga
    17521 gcagcgcaaa agtgtaagcg ccgatggcgt aaaagccgcc gtatcccagc accagcagac
    17581 cagaaagacc aacaaccacg ttcagcccga gaccgagaat aatgtagatc atggtcagag
    17641 tggcgatatc caccgtccca cgcgacacca taaacggcca cgccactgca agcaccagca
    17701 gcgccacgag gaacagtttc tgcttcaccg tggagccatc aatcgcgggc agaataaact
    17761 tcggcccgga aacgcttttt agccctttct ggaaagccgg tcgcacaagc tggaagaaaa
    17821 agaccaccgc cgtgccgata aacacccact gccaacggat ctctgaagcc gtgtcgacca
    17881 ccagtttggt gccatccagc tccagttgca cgcccataaa gacgcccgcc agcacaaaga
    17941 acatagcggc agagagcagc gccattgcaa tatgcatcgg tttcatactt tctctacctc
    18001 cgggcgaccc agaataccgg tcggcatcac cagcagcacc aggatcagca gggcgaatga
    18061 gaccacatct ttatattccg tactcagata ggcagaagag agcgcctccg caatccccag
    18121 gatcagcccg ccaatcatcg cccccggaat gctgccaatc ccaccgagca ccgcagcggt
    18181 aaaggctttc atcccggcca taaagccgat gtaagggtta attacgccgt agaactgacc
    18241 gagcagcacg cccgccactg ccgccatcgc cgcgccaatc acaaaggtca gcgcaatcac
    18301 ccggtcggtg ttgatgccaa gcaggctcgc cattttcaga tcttccgcgc aggcacggca
    18361 ggcgcgcccc atacgggaat agcgaatgaa aatcgtcagt gccagcatgg cgaggaaggt
    18421 gacaatccag ataaccgcct gcatggtagt aatagaggca gagaagtttt cgctatgacc
    18481 caccacccac tgaccgttaa acaggctggg cagcgccacg tcgcgcgaac cttcggtcag
    18541 gctaacgtag ttttgcagga agatagacat accgattgcc gagatgagtg caatcaggcg
    18601 cttagagttg cgcaccgggc gataggctac ccgttcgata ctccagccgt aggcgctggc
    18661 aatgacgatt gcgccgacga atcccgccgc caccagcagc cagccggtat caatgcccag
    18721 catcatcagt gcggcgatga tcataaagga gacgtagctg ccaatcatgt aaacctcgcc
    18781 gtgggcgaag ttgatcatgc cgataatgcc gtaaaccatg gtgtagccga tggctatcag
    18841 cgcgtaggta ctgcccagcg tgacgccgtt aaacatctgc tgcaagaaat acaaaaactg
    18901 ctcagacata aggtaacctt tctaaacccg cccgtttttc acggacggtg ggatgatcac
    18961 ttggctgccg tggatgaacc gtcggcgtgc cactggaaga caccaaaatc gaatccctta
    19021 agatcgcctt tttcatccca gttcagcggc ccaattacgg tgtttgcacc gttagctttt
    19081 aaatctttca ccagcgccag cggctcatcg ctgccggtac gttcaagggc agttgccaga
    19141 gattgcaccg ccgcgtaggt gatccagaca tagggcccgg acggatcttt cttgtcggct
    19201 ttcagcgcat caacgatgcc ctggtttgtc ggatcctggt catagcgttt tggcatggtg
    19261 accaacatgc cttcagcggc atcaccggca atgttcgaca gcgacgcatt accgacacct
    19321 tccggcccca taaactgggt tttcaggcca acggaacggg cctggcgcag catctgcccc
    19381 atttccgggt agtaaccgcc gtagtaaacg aagtcgatgt tttctttttt caggcgggcg
    19441 atcagcgcgg agaaatcttt ctctccagcg gtaatgccgt cgaagaagac gacgttggcg
    19501 ttagccgctt tcagcccgtc ctgcaccgaa cgtgccagcc cttcgccata ctgttgtttg
    19561 tcgtgaatga tggcgatgcg ctggggcttc accgtctcaa gaatgtattt tgccgccgtt
    19621 ggcccctggg aagagtccag cccggcagta cgcatgatgt attgataacc gcgttgggtc
    19681 agctccggat tggtcgctcc cggcgagatc atcagaatac cttcgtcttc atagatatct
    19741 gatgcaggtt gggtagagga ggaacacaga tgaccaataa catatttaat gccgtcgtta
    19801 acgattttgt tggcgaccgc aacggcttgt ttcgggtcgc aggcgtcgtc atattccacg
    19861 ccaaccagtt tatcgccctt aattccccct ttggcattaa tgtctttaat cgcctgacgc
    19921 gcgccgttaa attccatatc gccccactgg gcaatcgggc cggacatcgc gccgacaacg
    19981 gcgactttaa tatcatcagc catagcggtg tgtgaaattg tcagtgcaat catccctgcg
    20041 atgatcgttt tcgcattccg tttcatagtc aaaaatcccc attcgtgatg ttgtgttgct
    20101 ttgtttttat gtgttaacaa atcagactgt tcttttttta tactgcactg tttttacctg
    20161 tctgattaag ggtttagcgc agtattttgt gatgatagcg at
//
LOCUS       DAZKHK010000098        19869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKHK010000098 DAZKHK010000000
VERSION     DAZKHK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.098"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            120..686
                     /locus_tag="ACHOS5_003572"
     CDS             120..686
                     /locus_tag="ACHOS5_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778330.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFTTETNMETTFTGTNYAGP
                     ANADNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDTSTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
     gene            767..3415
                     /locus_tag="ACHOS5_003573"
     CDS             767..3415
                     /locus_tag="ACHOS5_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9778331.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDSGKYNLRVFINKQPLSDEYDINWYVSENDPTKNYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHNRSNDDDDDSSNSTTSKHWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADKHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNADEHMMVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            3435..4187
                     /locus_tag="ACHOS5_003574"
     CDS             3435..4187
                     /locus_tag="ACHOS5_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9778332.1"
                     /translation="MSYKLSCPMLVSTALMALLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRRPWQFKV
                     TLTRSGQDYTVNNPTSYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            4204..4707
                     /locus_tag="ACHOS5_003575"
     CDS             4204..4707
                     /locus_tag="ACHOS5_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778333.1"
                     /translation="MKAFFLTGSAALLLACGVTVFPAMAGGKIVNLTLRVLVDGPPPC
                     SVKGSAVEFGNVIIKSIDGANYRQPVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     TGISGFGIRIQNAADHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVELMASEFNATM
                     TMVVDYQ"
     gene            4704..5183
                     /locus_tag="ACHOS5_003576"
     CDS             4704..5183
                     /locus_tag="ACHOS5_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778334.1"
                     /translation="MKRLAWCLIYGFAGLAQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVS
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPVKANDAALSDGTFSAYATLQVDYQ"
     gene            5180..5707
                     /locus_tag="ACHOS5_003577"
     CDS             5180..5707
                     /locus_tag="ACHOS5_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778335.1"
                     /translation="MNLKRISHCAAALATVLLTPHVQAADNLHFYGNLLRKSCTLVVQ
                     GESLAEVHFPTVSRKDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDVSSAAQGVGIGMEKTDGTPVPINNTSGATFVLSNGNNNLNFNAWLQAKSGREVT
                     MGEFTAFLTATFEYI"
     gene            5709..6551
                     /locus_tag="ACHOS5_003578"
     CDS             5709..6551
                     /locus_tag="ACHOS5_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP9778336.1"
                     /translation="MKILRFWACLLVLIFSSTGGLAAKKIATLVTPIGFSAVTTQIHY
                     YLEILTPEGVAYGVYKQHSRMLQTEKLDLVSWSGNTTAPELTVDDGYEVAKSNCVGLE
                     AYDALSSRTNWICENMVLSVYHDGPVQGCPWLVSTYTDSVLPYDVPTPYVGPKVHNSS
                     CPVSVATYDISWSEDYVVHSKVLSLQSTGGTIEKTLPTFLMESGKLCDGRVMDDRGAY
                     CRFVAQMITFSTSGCDSSKVTVTPNQHPITDKQLHDMVVRVDTSSRQPIDSTCRFQYT
                     LNEL"
     gene            complement(6871..7422)
                     /gene="smrB"
                     /locus_tag="ACHOS5_003579"
     CDS             complement(6871..7422)
                     /gene="smrB"
                     /locus_tag="ACHOS5_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP9778337.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7588..8520
                     /gene="prmB"
                     /locus_tag="ACHOS5_003580"
     CDS             7588..8520
                     /gene="prmB"
                     /locus_tag="ACHOS5_003580"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9778338.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            8555..9640
                     /gene="aroC"
                     /locus_tag="ACHOS5_003581"
     CDS             8555..9640
                     /gene="aroC"
                     /locus_tag="ACHOS5_003581"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9778339.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9644..10468
                     /gene="mepA"
                     /locus_tag="ACHOS5_003582"
     CDS             9644..10468
                     /gene="mepA"
                     /locus_tag="ACHOS5_003582"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP9778340.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10468..11277
                     /locus_tag="ACHOS5_003583"
     CDS             10468..11277
                     /locus_tag="ACHOS5_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9778341.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11277..11825
                     /gene="epmC"
                     /locus_tag="ACHOS5_003584"
     CDS             11277..11825
                     /gene="epmC"
                     /locus_tag="ACHOS5_003584"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP9778342.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQGQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11859..12137
                     /locus_tag="ACHOS5_003585"
     CDS             11859..12137
                     /locus_tag="ACHOS5_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP9778343.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(12258..14264)
                     /gene="mnmC"
                     /locus_tag="ACHOS5_003586"
     CDS             complement(12258..14264)
                     /gene="mnmC"
                     /locus_tag="ACHOS5_003586"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP9778344.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRITLDLWFGDINEL
                     TSKLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAN
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQHQLQDLSRKDDGWLLNFAGDQ
                     HATHSVVVLANGHQISGFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPQVQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14423..15643
                     /gene="fabB"
                     /locus_tag="ACHOS5_003587"
     CDS             14423..15643
                     /gene="fabB"
                     /locus_tag="ACHOS5_003587"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP9778345.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15908..17086
                     /locus_tag="ACHOS5_003588"
     CDS             15908..17086
                     /locus_tag="ACHOS5_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9778346.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGVLLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATMFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17083..18078)
                     /gene="flk"
                     /locus_tag="ACHOS5_003589"
     CDS             complement(17083..18078)
                     /gene="flk"
                     /locus_tag="ACHOS5_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP9778347.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(18403..18543)
                     /locus_tag="ACHOS5_003590"
     CDS             complement(18403..18543)
                     /locus_tag="ACHOS5_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9778348.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(18861..19001)
                     /locus_tag="ACHOS5_003591"
     CDS             complement(18861..19001)
                     /locus_tag="ACHOS5_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9778349.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHKSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            complement(19112..19447)
                     /locus_tag="ACHOS5_003592"
     CDS             complement(19112..19447)
                     /locus_tag="ACHOS5_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778350.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSGDE
                     NEKIRKARTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKQPRHPLHVTDVKGKLRAGA
                     FRLTAGRIT"
BASE COUNT         4649 a         5159 c         5327 g         4734 t
ORIGIN      
        1 attctatgat tatattggag tgtatatgtt caatggctaa ctataaatat cgggcataat
       61 atatattaat tttatgtcag gtgcttatat tataactctt aatgatttaa ggacaaataa
      121 tgagtaagtt tgccaaaaca gccattgctg cggcgatggt catgggcgca gttgtttcca
      181 gttctgcttt tgcagcaggc aacaacggta ccgcgcgctt ctacggcacc attgaagact
      241 ccccgtgctc tatcgttcca gacgatcaca aactggaagt cgatatgggt gacatcggtg
      301 cggctaaact gaccggcggt ggcactacca caccgaaaga tttccagatc cgtttacagg
      361 attgcgtgtt taccactgaa acaaacatgg agaccacgtt caccggcact aactacgcgg
      421 ggccagccaa cgcagataac tatgcgttat tcaatgttga cagcggtgtt gccatgaacc
      481 acgtgagcct ggtaatcggc gatacccatg gcaaaggtta taaagtgggc gaaaagattg
      541 ttcagccaat cgtgatggat acttcaacca gcaagggtaa agacaagcaa acgctgaact
      601 ttaaagcctg gctggtgggt gaaaccgatg cgccggatct cggccagttc gaaaccctga
      661 ccacattcca gattacctat ctctgatgtg acaaccccac agcgttctgc tgtggggtta
      721 ttcaacacgc aacagattta cgactgtttc acaaacggat atgtgtatgc ctaatcactc
      781 aaattttcgg ctgcggggaa tcgcctgcta tattgcgctg gcaatctctg gtgggtcagt
      841 caatgcatgg gctgatgatt caattcaatt tgacccccgt ttccttgagt taaagggcga
      901 tacgaaaatt gatctcggta agttttcaaa aaaagggtat gtcgactcgg gtaaatataa
      961 tttacgtgta tttataaata aacaacccct ttctgatgaa tacgacatta actggtacgt
     1021 ctctgaaaac gatccaacaa aaaactatgc ctgcctgaca cctgagttag tggcggcgct
     1081 ggggctgaaa gaagggatag caaaaagcct gcagtggacg cacaacgatg aatgccttaa
     1141 accaggtcaa ttagacggga tggaagtcga gaatgattta agccagtcgg cgttgctgct
     1201 gacagtgcca caggcttacc tcgaatatac cagcagcgac tgggatcctc cctcacgctg
     1261 ggacgacggt attcctggcc tgattgccga ctacagcctc aatgcgcaaa ctcgccacca
     1321 ggagcagggg ggcgaggact cacatgatat cagcggcaac ggtaccgttg gggcgaacct
     1381 gggggcatgg cgtttccgtg cagactggca aagtgattat cagcacaacc gcagcaacga
     1441 tgacgacgat gacagcagta acagcacaac gagtaaacac tgggactgga gccgttatta
     1501 cgcctggcga gccttgccct ctttaaaagc gaagctgtcg ctgggggaag attatctcaa
     1561 ttccgatatt ttcgacggct ttaactatat cggcagcagc gtcagcaccg atgaccaaat
     1621 gctgccgccc aacctgcgcg gctatgcacc ggatgtttcc ggcgtggcgc acagcagtgc
     1681 aaaagtcacc attagccaga tgggccgggt actttacgaa acccaggttc ccgccgggcc
     1741 attccgcatt caggatatcg gcgactccgt ctccggcaca ctgcacgtcc gcgttgaaga
     1801 acagaatggt caggtgcagg aatatgacgt tacgaccgca tctatgccat tcctcacgcg
     1861 ccaggggcag gtgcgttaca aagtgatgat ggggcgtccg gaagactgga accacaagac
     1921 cgaaggcggc tttttctccg gcggagaagc gtcatggggg gtggcagatg gctggtcgct
     1981 ctacggtggt gcgctggcag ataaacacta tcagtcggcg gcgatggggg tggggcgcga
     2041 cctcgcacag tttggcgcgc tggcgttcga tgtgactcac tcgcacgtca acctggatca
     2101 tgacagcgca tacggcaaag gaaaactgga cggcaactcc tttcgcgtga gctatgccaa
     2161 agactttgac gaactcaaca gccgcgtcac ctttgcaggc taccgttttt ctgaaaagaa
     2221 cttcatgacc atgagcgagt atctggacgc gaaccagtcg gacatggcgc ggaccggtaa
     2281 cgacaaagag atgtatacga tcacctataa ccagaacttt gccgctgcgg gtgtttcgat
     2341 ctatctcaac tactcccatc gtacttactg ggatcgcccg gaacagacaa actataacct
     2401 gatgttttcc cactatttta atatggggag cattcgcaac atgagcatct cggtgaccgg
     2461 ctatcgctac gaatatgacg ataatgcgga taaggggatg tacctcagta tgagcattcc
     2521 gtggagcgac agcagcaccg tgacctacaa cggttcctac ggcagcggat cggacagcag
     2581 ccaggtcggt tactttaagc gcgtcgatga cgcaacgcac taccaggtta acgttggcac
     2641 cagcgagcag cacggcagcg tggatggtta tctgagccac gacggttcgc tggcgaaggt
     2701 tgatctcagc gccaactacc atgaagggga ataccgttcg gcggggatcg ccttacaggg
     2761 cggggcaacg ctgaccgcgc atggtggggc gctgcaccgt acccagaaca tgggcggcac
     2821 gcgcctgctg attgacgccg acgggattgc caacgtgccg gtcgaaagca acggcgcgcc
     2881 ggtgtacacc aatatgtttg gcaaggcggt ggtcgccgat attaacaact actatcgcaa
     2941 tcaggcgtat atcgatttaa acaacttgcc ggaagatgcg gaagccaccc agtcggtggt
     3001 gcaggcgact ctgacggaag gcgcaatcgg ctatcgtaaa ttcaaagtga tcagcgggca
     3061 aaaagcgatg gcggtactgc ggttgcgtga cggtagctac ccgccgtttg gcgcggaagt
     3121 gaaaaacgac gagcagcagc aggttggcat tgtggatgat gaaggcaatg tctatctggc
     3181 gggggtcaat gcggacgagc atatgatggt gttctgggaa ggcagcgcac aatgcgagat
     3241 cgtattgccg aagccgctac ctgccgattt gttcagcggc ctgttgttgc cgtgtgaaca
     3301 aaagggaacg gcagcccctg attcttcagc gccagaaatt aagcctgtta ttcaggacca
     3361 gacgcggcaa gtcacaccaa cggaagcgcc gacgtcaatt tcagcaactc aataatctga
     3421 ttaaggaacg atcaatgtct tataaattat cctgtccaat gctcgtcagc acagcgctga
     3481 tggcactgct taccaccgcc agcctgacgg cgcatgcatc ggtcaccccg gatcgcaccc
     3541 gtctggtgtt taacgaaagc gataaatcga tcagcgtgac gttgcgtaac aacaacgaaa
     3601 agctgcctta cctggcgcaa agctggctgg aagacgagaa gggcaacaaa atcacttcgc
     3661 cgctggcagt actgccgccg gtgcagcgta ttgacgctat gatgaacggc caggtgaaga
     3721 ttcaggcgct gccggacatt cacacactgc cgtccgaccg agaaagcctg ttctactaca
     3781 acgtgcgtga gatcccgcca aagtccggga aagccaacac cctgcaaatt gcgctgcaaa
     3841 cgcgcatcaa gctgttctgg cgaccaaagg cgctggagaa gattgacatg agaagaccgt
     3901 ggcagtttaa ggtcacactc acgcgcagtg gccaggacta taccgtcaat aacccgacgt
     3961 cgtaccacgt catcatcagc gatgccagca cacagaagaa agggctgact gccgccgggt
     4021 ttaaaccgct ggtgatgcca ccgaaaacgt cacaaccgtt aaaggcaaaa atggcgagcg
     4081 ccccggtgct gacctatatc aacgactacg gcgcgcgcat gccgctgatc ttccgttgtg
     4141 aagggaacac ctgcaaggtg gatgaagatc aaagcagcaa aggttgataa cgggagaagc
     4201 aacatgaaag cgttctttct gaccggatct gccgcgctat tgctggcctg tggcgtgacc
     4261 gttttcccgg caatggcggg cggcaaaatc gtcaatttga cgttgcgcgt gctggtggat
     4321 ggaccgccgc cctgttcggt aaagggaagc gcggtggagt tcggcaatgt gattattaaa
     4381 agcattgatg gtgccaacta ccgtcagcca gtgggctaca ccctgaactg ctcaaacagt
     4441 gtcagcgacg acctgcaaat gcagcttcag gccaccacca cgaccatcaa cggcgaaacg
     4501 gtgctgagta ctggaatcag cggttttggc attcgtattc agaatgccgc tgaccattcg
     4561 ctggtggcag tgggtaacag tagctggctg ccgttcaaca tcaatacaca gccagacttc
     4621 gaagccgttc cggtgaagca gagcggtgtg gaactgatgg catcggagtt taatgcgacc
     4681 atgaccatgg tggtggacta ccaatgaaac gactcgcatg gtgcttaatt tacggttttg
     4741 ctggcctggc gcaggccgcg attaacgacg ttacttttca cggcacgctg gtttcgccgc
     4801 ccgcctgtac tatcagcgat ggtaagacga tcgaagttga gtttcgcaac gtcattatcg
     4861 acaacattaa cggcgataat ttccgtcagg atgtgcctta caccatcaca tgtgaccccg
     4921 atgtgcgcga cgacgcctgg gagatgagcc tgacctggac gggcagccaa accccttatg
     4981 atgattccgc cattgaaacg gacgttagcg gcctggggat tgagctacag caaaacggcc
     5041 agccgtttaa gctgggaaca ccgttgaaaa ttgacccgtc aacgccgcca acgctgcaag
     5101 ccgtaccggt gaaagccaat gatgcggcgc tcagcgacgg aaccttttct gcctacgcaa
     5161 cattacaggt ggattaccaa tgaacctaaa acggatatct cactgcgcgg ctgcactggc
     5221 gacggtgctg ctgacgcccc atgttcaggc ggctgacaac ctgcatttct acggcaattt
     5281 actcagaaag tcctgcacgt tagtcgttca gggcgagtcg ctggcggagg tgcatttccc
     5341 caccgttagc cgcaaagacc tgatggtgac cgggcagtcg gcgcgggtgc cggtagtgtt
     5401 tcagttgaaa gattgtaaag ggccagccca gtatgaggtt cgggtgacgc tgaccggtac
     5461 agaagacagc gaacagcccg gttttctggc gctggatgta tcttcggcgg cgcaaggtgt
     5521 cggaattggc atggaaaaaa ccgatggaac accggtgccg attaacaata ccagcggcgc
     5581 gacctttgtg ctgagtaacg gcaataacaa tctgaatttt aatgcctggc tccaggcgaa
     5641 aagcgggcgg gaagtgacga tgggtgaatt taccgctttt ttaaccgcaa cgtttgagta
     5701 catttaagat gaaaatactg cgtttttggg cctgtttact cgtcctgatc ttttcaagta
     5761 ctggtgggct ggcggcgaag aaaattgcaa cattggtgac tccgatagga ttttctgctg
     5821 ttacaaccca gattcattac tatcttgaaa ttctgacgcc agaaggcgtt gcctatggcg
     5881 tgtacaagca acacagtcgt atgcttcaaa cagaaaagtt agatttagtc agttggagcg
     5941 gtaacacgac tgcacctgag ttgacagttg atgatggcta tgaggtagca aaatccaatt
     6001 gtgttggact tgaagcatat gatgctttat cgagtaggac gaattggatt tgtgagaata
     6061 tggtccttag cgtgtatcac gatggacctg tacaagggtg cccatggtta gtttcaacat
     6121 acactgatag tgttctcccg tatgatgtac cgaccccata tgtggggcca aaagtgcata
     6181 actcgtcatg tcctgtctcc gttgccactt atgatatttc gtggagtgaa gattatgttg
     6241 tccattcgaa agtgctgtcg ctgcaatcga cgggcggaac gattgagaaa acgctgccta
     6301 cctttttgat ggaaagcggc aagctctgtg acggcagagt gatggacgat cgaggtgcat
     6361 actgccgctt tgtggcgcaa atgatcacct tctccacctc cggctgtgac agttcgaaag
     6421 tgacggtcac acccaaccag catcccatca ctgacaagca actgcacgac atggtagtgc
     6481 gtgtcgatac cagcagccgc cagccgattg attccacctg ccgcttccag tacacgctca
     6541 acgagctgta gcgtatacac cacacaacgg ggccatcagg ccccgttgtc cgttatgtca
     6601 tgcggtcatc cagtttttta gaatattcgc catcacggga gcgaaaggtg ccagataacc
     6661 gtcgacgagg gtgagtaatt cgtcgataag cgcttgcagc aggtcgtcat cctcaagccc
     6721 ctctgaaggg tcaatgccat tagcccacag aaggctgtcg gaaagctgct tacgtttttc
     6781 aattaatgcc cgattattac gttcattatc gccagggttg agacgccagt atccaaggct
     6841 aaatggcccg actcatccac cttttaatat tcagggtaat tccggcggca accactcttc
     6901 cacttcaatc agcaccaaca acgccgcatc gccaccatac tcttttggtg cctggtgaaa
     6961 tgccatcaca tgcggatgtt gcgccagcca cagcggcgtt tgctgcttca aaatatgctt
     7021 cccgtgccca tgcatcacgc aggcgcaaaa cacatgttca cggcggcagg cggcaatcaa
     7081 cgcccccagt tcctgtttgg cttgtagctg cgtcagaccg tgcaaatcaa gaaacaactc
     7141 cggtgaataa tcgccacggc gtaacttctt cgcctcaaaa tggctgacat ccggtcgaac
     7201 atatttgacc ggaccttcgg tattcaataa tggctgaaat tcgtccgaaa aatagtggct
     7261 ggcatcggcc tgttcctgaa tcaaccgctt tactggcact tcgctgattt ttttacgctg
     7321 cggtcggtgg acaatcgtgt cctgcttaat cttgcgagta cccgccatca actggcgaaa
     7381 cagcgcctgg tcctcctcgc taagtgttgt tttctttttc atttatctgt ctctatttct
     7441 aattttccta cagtttaccc gactcaccgt acaatcgatc gcttaaaacg tatttttcct
     7501 cactcaccgc gtgtgagtgc tgatttatcg tcatcttcat ggcaaactag ccgccgaaat
     7561 tattgcgatc atgccctgga ggaatacgtg gataaaattt tcgttgatga agcagtaaat
     7621 gagctgcaaa ccattcagga catgttgcgc tggtcggtga gccgcttcag cgcggcaaac
     7681 atctggtacg gccacggcac cgataacccg tgggatgaag ccgtgcaact ggtgttgcct
     7741 tcgctctact tgccgctgga tattccggaa gatatgcgca ccgcgcgtct gacctccagc
     7801 gaaaaacacc gcattgttga gcgtgtgatc cgccgcgtca acgagcgcat tccggtggcc
     7861 tacctgacca acaaagcctg gttctgcggc catgaatttt acgtcgatga acgcgtactg
     7921 gtgccgcgct cgccgattgg tgaactgatc aacaataaat ttgccggact catcagcaag
     7981 caaccgcagc acattttaga tatgtgtacc ggtagcggct gcatcgccat tgcctgtgct
     8041 tatgccttcc cggaagcgga agtcgacgcg gtggacatct ctccagacgc gctggcggtt
     8101 gctgaacaga acatcgaaga acacggtctg atccataacg tcattccgat tcgttccgac
     8161 ttgttccgcg acttgccgaa agtgcagtac gacctgattg tcactaaccc gccgtatgtc
     8221 gatgcggaag atatgtccga cctgccaaac gaataccgcc acgagccgga actgggtctg
     8281 gcatctggca ccgacggcct gaaactgacg cgtcgcattc tcggtaacgc ggcagattac
     8341 ctcgctgatg atggcgtgct gatttgtgaa gtgggcaaca gcatggtaca tcttatggaa
     8401 caatatccgg atgttccgtt cacatggctg gagtttgata acggcggcga tggtgtgttt
     8461 atgctcacca aagagcagct gctggcggcc cgtgaacatt tcgcgattta taaagattaa
     8521 gtaaacacgc aaacacaaca ataacggagc cgtgatggct ggaaacacaa ttggacaact
     8581 ctttcgcgta accaccttcg gcgaatcgca cgggctggcg ctcggctgca tcgtcgatgg
     8641 tgttccgcca ggcattccgc tgacggaagc ggacctgcaa catgaccttg atcgtcgtcg
     8701 ccctgggaca tcgcgctata ccacccagcg tcgtgagccg gatcaggtca aaattctctc
     8761 cggtgttttt gaaggtgtta ctactggcac cagcattggc ttgttgatcg aaaataccga
     8821 ccagcgttct caggattaca gcgcaattaa agacgtcttc cgtccaggcc atgccgatta
     8881 cacttacgaa caaaaatacg gcctgcgtga ttatcgcggc ggtggacgtt cttccgcccg
     8941 tgaaaccgct atgcgcgtgg cggcaggggc gattgccaaa aaatatctcg ccgagaaatt
     9001 tggcattgag attcgcggtt gcctgaccca gatgggcgac attccactgg aaatcaaaga
     9061 ctggtctcag gtcgagcaaa atccattctt ctgcccggac ccggacaaaa tcgacgcgtt
     9121 agatgaactg atgcgcgcgc tgaaaaaaga aggcgactct attggtgcga aagtcaccgt
     9181 cgttgccagt ggcgtacccg ccggacttgg cgagccggtc tttgaccgcc tggatgccga
     9241 catcgcccat gcgctgatga gcattaacgc ggtgaaaggc gtggaaattg gtgatggttt
     9301 tgacgtggta gcgctgcgtg gcagccagaa ccgcgacgaa atcaccaaag acggtttcca
     9361 gagcaaccat gcgggcggca ttcttggtgg tatcagcagc gggcagcaaa tcattgccca
     9421 tatggcgctg aagccaacct ccagcattac cgtgccgggg cgcactatta accgctttgg
     9481 cgaagaagtt gagatgatca ccaaaggccg tcacgatcct tgtgttggga tccgcgcggt
     9541 gccgatcgca gaagcgatgc tggcgatcgt tttgatggat cacctgctac ggcagcgggc
     9601 gcaaaatgcc gatgtgaaga ctgatattcc acgctggtaa aaaatgaaca aaaccgcgat
     9661 tgcgctgctg gctctgcttg ccagtagcgc cagcctggca gcgacgccgt ggcaaaaaat
     9721 aacccaacct gtgccgggta gcgcacaatc gataggcagt ttttctaatg gctgtatcgt
     9781 cggtgctgac acgttgccga tacagtccga acattatcag gtcatgcgta ccgatcagcg
     9841 tcgctatttc gggcacccgg atctggtgat gtttatccag cgtctgagtc gccaggtgag
     9901 caatctgggc atgggtacgg tgctgattgg cgatatgggg atgcctgctg gtggtcgttt
     9961 caacggcggt cacgccagcc accagaccgg actggatgtc gatatcttcc tgcaactgcc
    10021 aaaaacacgc tggacctccg cgcagctctt gcgcccgcaa gctctggact tagtttcccg
    10081 cgacggtaaa cacgttgtcc ccgcgctgtg gaagccagaa attttcagct tgatcaaact
    10141 cgccgcccag gacaaagacg tcacgcgcat ttttgttaat ccggcgatta aacaacaact
    10201 ttgtcttgat gcgggcaccg atcgcgactg gttgcgcaaa gtgcgaccct ggttccagca
    10261 tcgcgcgcat atgcatgtac gattacgttg tcctgccgat agtctggagt gtgaggatca
    10321 acctttaccg ccaccaggcg atggttgcgg ggcagaactg caaagctggt ttgaacctcc
    10381 aaaaccggga acaacaaagc ctgagaagaa gacaccgcct ccgttgccgc cttcctgcca
    10441 ggcgctactg gatgagcacg tgatctaatg gaaacgttta atagcctgtt tatggtttcc
    10501 ccgctgctgc tgggagttct cttttttgtc gccatgctgg cgggatttat cgactcgatt
    10561 gccggtggcg gtgggttact caccattccg gcattgatgg cggcggggat gtctcccgct
    10621 aacgcgctgg caaccaataa actgcaagcc tgcggtggct ctatttccgc cactatctac
    10681 tttattcgcc gtaaagtggt tagtttaagc gatcaaaaac tcaatatcgc catgaccttt
    10741 gtcggctcaa tgagcggcgc gctactggtg cagtatgttc aggccgatgt cttacggcag
    10801 attttgccta ttctggtgat ttgtatcggc ctctatttct tgctgatgcc aaagctgggt
    10861 gaagaagatc gccagcgtcg gatgtatgga ctgccatttg cgctggtggc ggggggctgc
    10921 gtcggttttt acgatggatt ctttggcccg gcagccggat cgttttacgc gttagccttc
    10981 gttacgctgt gcgggtttaa tctcgccaaa gccacggctc acgccaaatt actcaacgca
    11041 acgtcaaata tcggcggttt actgctgttt attcttggcg gcaaagtgat ttgggcgaca
    11101 ggctttgtga tgttggtggg gcagttcctc ggcgcgcgca tgggttcaag actggtgttg
    11161 agcaaaggac aaaagctgat ccgcccgatg atcgttatcg tctcggcggt gatgagtgcc
    11221 aaattacttt atgatagcca cggacaggag atcctccact ggttggggat gaactaatga
    11281 acagtacaca ccactacgag cagttgattg aaatttttaa tagctgcttt gccgatgaat
    11341 ttaatacccg tctgattaaa ggcgacgacg aaccgatcta tcttcctgct gatgctgaag
    11401 taccatataa ccgaatcgtc ttcgctcatg gcttttatgc cagcgccatc cacgagattt
    11461 cgcactggtg tattgccggg aaagcgcgcc gtgagctggt tgatttcggt tactggtatt
    11521 gcccggacgg tcgcgatgcc caaacgcaag gccagtttga agatgttgaa gtgaagccac
    11581 aggcgctcga ctggttgttc tgtgtggcgg cgggatatcc gtttaacgtc agttgcgaca
    11641 atctggaagg ggatttcgaa ccggatcgtg tggtgtttca acggcgggta catgcgcagg
    11701 tgatggatta tctggcaaac ggcattcccg aacgtccggc acgcttcatt aaagcgctac
    11761 aaaattatta tcacacgccg gaacttacgg cggaacagtt cccgtggccg gaagcgctca
    11821 actaaagcca gcgccacgtt ttacaatgag gaaagaagat gatcgcggag tttgaatcac
    11881 gcattctggc attaatcgac ggtatggttg accatgccag tgatgatgag ttgtttgcca
    11941 gtgggtattt gcgtggccac ctgacattag ccatcgcaga actggaaagt ggtgacgacc
    12001 actccgctca ggcggtacat acaaccgtta gccagagtct ggaaaaagcc attggcgcgg
    12061 gtgaattgtc accgcgtgac caggcgctgg ttaccgatat gtgggaacac ctgtttcagc
    12121 aggcctcaca gcagtaagtt gtgacgtctg gagcaccatg cctgatgcga cgcttatcgc
    12181 gtcttatcag gcctacaggt aacgcgtagg tcggataagg cgtttacgcc gcatccgaca
    12241 aacaatgcca aagcagatta ccccgcctta accgctttac ctttcaacaa cttccgcacc
    12301 cataacctat tcggatttaa cgccgccagc gtactggcat ccatcggaat cggttcttca
    12361 ctcatctgtg ctgccagaat ctcggcacac agcggggcag aacacaaacc gcgcgaacct
    12421 aaagcagcaa acataaagag atcgtcataa accggcgcgc ttaccgcttc atctttcttc
    12481 tccgccagcg acgcatattc cacgagtgta gcgtcataat caggcacgtt gcctaccatt
    12541 ggcagatgat cgcgagtggc acaacgcaca ccgcagcgcg cctctttacc actgacatca
    12601 acctcttttg cccactgtac ctgcgggaaa caatcaatca accgctggcg attctgctgc
    12661 tgatcttcat cactgtacgc cgtctcttcg ctaccacgat gataactggc accaatacaa
    12721 tgatgctgat tcgccggatt ttgcggcgtg agataaccgt catagcacag cacttgcttc
    12781 agtttcgcca gctccggcgt tgtcggaata tggctgacct gcccggcaac cgaatatact
    12841 ggcagagatg atgtttggct gaaaccgctg atttgatgcc cattcgccag taccaccaca
    12901 ctatgcgtcg cgtgctgatc acctgcaaaa ttcaacaacc aaccgtcatc cttacgggat
    12961 aaatcctgta actgatgttg ataatgaatc tgcaaaccct gctgttgcgc cagttccagc
    13021 acattacggg tcagttctgc cggacacagc cagccacctt gcggataagt aataccgctg
    13081 caatttgtcg caacgcccgt aatttgttca accgcatttg cctcaacggc tacagccagt
    13141 tctgcgggta aatccatcga caacatctgt gcgattttat gctggctttt ctcatcccag
    13201 cctaactgcg tgacgccgca ccagtcatga tcaaatttaa cgggcaataa atcgtaaaac
    13261 cgacgagcaa aagtaaacgc attagagaaa aaacggttta gcgcttcatc gtgtttactt
    13321 aaaagcggat acagcgcccc ctggcgattg ccggaagcac ccagcgcggg ggcttcatcc
    13381 gcgcaataaa gcgttacttg ccagccgcgc cgtaataacg ccagcgacaa caacgcgttg
    13441 gcaataccac cgccgataat cgctacttcc cgtttgctgc tgcccgtacg attaaaccac
    13501 ggcgtggagc aggggagggg taatgtctgt tccatcaccc cacaaagcat ttcccgtttg
    13561 cgcccaaagc ccttacgttt ttgcatcgtg aatccggctt cctgcaaacc gcggcggaca
    13621 aaaccggcag acgtaaaggt cgccagcgta ccgccaggac gcgccagtcg cgccatggcg
    13681 ttaaacagat tttgtgtcca catatccggg tttttcgctg gcgcaaagcc gtccagaaac
    13741 caggcatcta ctttttgatt tagcgaatcg tccagtttgc tggtcagttc gtttatatcg
    13801 ccaaaccata aatccagcgt tatgcggcct tcatcgagca ataaacgatg gcaaccgggc
    13861 aggggcaaag gccactgcgc ctgaagctgt tccgcccacg gggccagctc cggccagtgt
    13921 tgatgcgcca gcgctaaatc cgcacgggtg aggggaaatt tctcaaaact aatgaaatgt
    13981 aagcgttgta attgcgcctg tggatgcgct tcgcgaaact gatcaaatgc ctgccatagc
    14041 gtcaggaagt ttaatccggt gccgaagccg ctctctgcta ccacaaacag aggatgtgga
    14101 tgttcaggaa aacgtgcctc taaatggtta cccccgagaa aaacataacg cgtctcttcc
    14161 agtccgttat cgttggaaaa atagacatcg tcaaaatctc gggaaacagg tgtaccctca
    14221 gcattaaatt cgaggttggc aggttgtatg gagtagtgtt tcacgtaagt tactcgtctt
    14281 acaggcggtg gctcgatctt agcgatgtgt gtcaggctgc gcaaatttct ctattaattg
    14341 gctgatcgga cttgttcggc gtacaagtgt acgctattgt gcattcgaaa cttactctat
    14401 gtgcgactta cagaggtatt gaatgaaacg tgcagtgatt actggcctgg gcattgtttc
    14461 cagcatcggt aataaccagc aggaagtcct ggcatctctg cgtgaaggac gttcagggat
    14521 cactttctct caggagctga aggattccgg catgcgtagc cacgtctggg gcaacgtaaa
    14581 actggatacc actggcctca ttgaccgcaa agttgtgcgc tttatgagcg acgcatccat
    14641 ttatgcattc ctttctatgg agcaggcaat tgcagatgct ggcctctccc cggaagctta
    14701 ccagaataac ccgcgcgttg gcctgattgc aggttccggc ggcggttccc cgcgtttcca
    14761 ggttttcggc gctgacgcaa tgcgcggccc gcgcggcctg aaagcggttg gcccgtatgt
    14821 ggtcaccaaa gcgatggcat ccggcgtttc tgcctgcctc gccacgccgt ttaaaattca
    14881 cggcgttaac tactccatca gctccgcgtg tgcgacttcc gcacactgta tcggtaacgc
    14941 agtagagcag atccaactgg gcaaacagga catcgtgttt gctggcggcg gcgaagagct
    15001 gtgctgggaa atggcttgcg aattcgacgc gatgggtgcg ctgtctacta aatacaacga
    15061 caccccggaa aaagcctccc gtacttacga cgctcaccgt gacggtttcg ttatcgctgg
    15121 cggtggcggt atggtagtgg ttgaagagct ggaacacgcg ctggcgcgtg gtgctcacat
    15181 ctatgctgaa atcgttggct acggcgcaac ctctgacggc gcagacatgg ttgctccgtc
    15241 tggcgaaggt gcagtgcgct gcatgaagat ggcgatgcac ggtgttgata ccccaatcga
    15301 ctacctgaac tctcatggta cttcgactcc ggttggcgac gtgaaagaac tggcagctat
    15361 ccgtgaagtg ttcggcgata agagcccggc gatttctgca accaaagcaa tgaccggtca
    15421 ctcactgggc gctgctggcg tacaggaagc tatctactct ctgctgatgc tggaacacgg
    15481 ttttatcgcc ccgagcatca acattgaaga gctggacgag caggctgcgg gtctgaacat
    15541 cgtgaccgaa acgaccgatc gcgaactgac cacagttatg tctaacagct tcggcttcgg
    15601 cggcactaac gccacgctgg taatgcgcaa gctgaaagat taattcggag taggtcggat
    15661 aagacgcgcc agcgccgcat ccgacgttac gcgccaatgc ggcctccggc actaacgcaa
    15721 aagggaaccc gatggttccc tttttcacat cattgacaat cgccgccagt tccaggcaaa
    15781 cttcccgctt tgtcgatttc cttcagaaaa gacgtacgcg ttaaatcctg ccaacgcacc
    15841 gtaaccctga aatcagagag atgagacggg gatactcctc gccttgcgct gcattctgga
    15901 gtaatgcatg actgctgtaa gccaaaccga aacacgatct tctgccaatt tttcgctctt
    15961 ccgcatcgct tttgcggttt ttctcaccta catgaccgta ggtttaccgt tgccggttat
    16021 cccgctgttt gttcatcatg aactgggcta tggcaacact atggtcggca ttgccgtcgg
    16081 gattcagttt ctggctacgg tgctgacgcg tggttacgcc gggcgactgg ccgatcaata
    16141 tggcgcaaaa cgttcggcgc ttcaggggat gttagcttgt ggtctggcgg ggggcgtgtt
    16201 gctgctggcg gcgattttgc ccgtccccgc accgttcaaa tttgctctgt tggtcgtcgg
    16261 gcgtttgatt cttggctttg gtgaaagcca gttactgacg ggcgctctga cctggggatt
    16321 gggcatcgtt gggccaaaac attccggcaa agtgatgtca tggaacggaa tggcgattta
    16381 cggtgccctc gctgttggtg cgccgcttgg cctgttgatt catagccatt acggttttgc
    16441 cgcactggcg ctcaccacaa tggcattacc cttactggcg tgggcctgta acggcacagt
    16501 gcgcaaagta cctgccctgg cgggagaacg tccatcgctg tggagcgttg tcgggcttat
    16561 ctggaaacca gggttaggtc tggcactaca aggcgttggt tttgcggtta tcgggacttt
    16621 cgtttcgctc tactttgcca gcaaaggatg ggcgatggcg ggctttactc ttaccgcgtt
    16681 tggcggcgca tttgtcgtga tgcgcgtcat gtttggctgg atgccggacc gttttggcgg
    16741 cgtgaaagtg gcgatcgtct ctctgcttgt agaaacggtg ggcttgttgc tgctctggca
    16801 agccccaggg gcgtgggtcg cattagcggg cgcggcgtta actggagccg gatgttcgct
    16861 tatctttcct gcgctgggcg tggaggtggt taaacgcgtc ccctcacacg ttcgcggcac
    16921 cgcactgggc ggttacgccg cgtttcagga tatcgccctc ggcgtctccg ggccgcttgc
    16981 gggaatgctg gcgaccatgt ttggttactc ttcggtattt cttgccgggg cgatctctgc
    17041 ggtgctggga attattgtca cgatactgtc gtttcgtcgg ggttaacgaa ccagccagac
    17101 cagcatcagg ataatcgcga ctaacagtat ccacagcgcg ggacgcgttg ccatattttg
    17161 caacgtgtcc atcatcggtg gaaaaggatt aagcagcggt tgcatatcac gcggctcaat
    17221 ccctttaacc cgccgttgat aaagctggtt aagaatatcc tcggcctggg cagaagaaag
    17281 cgatgattgc gcagaaaggc catatttttg ctggatatat gccgataacg ccgccagttc
    17341 actggcatct aaaggttgtt ttagcgccat ctgtagtgat tccagtgtcg gcgtattttg
    17401 ctggcttagc gtctgacgcg cctgtagcca ggtcaccaga tggttaaaca gtttcgctgg
    17461 aattaactcg ccatctttca ccccggaaag ttccagcatc gattgccaga tctgtttgct
    17521 gggttccccc gttgctgccg caagtttggt caccagctgt ttgagcgcat tgtgctccgc
    17581 cggtaataaa ggacggtcgg tcgcctcgcg ctgctgtggt tgcggaataa ccatcttccc
    17641 ttcctgcaac agggtgagaa tggtttttaa ttgctccggt gagagctgat tcagcggcgt
    17701 ctgaccaaag ttatgacgga tataatccgt gaccgcctga cgattattcc ccagacgtaa
    17761 atactcccct aattgcgcta aaagctggcg ggcagaatgg cttttttgcg cggccaacag
    17821 acgttgcgcc agattatgct cagcggcagg gaagtgacgc gaaagcagcg gtgaatctcc
    17881 cgacagacca atatcgtgcc tgatacccgc ccacagttct gctctttgtt gttgcgtcag
    17941 tgaggtcact ttcgtcatta agctttccag cgaagtacgt tgctgactgg ataaaggctg
    18001 attgcctgcg ccagacggca ggttatctcc ctgacctggt ggttgcccag gaggagggcc
    18061 ggaaataggt tgtatcatta cgtatcctta tacctgaaat cttcgcaagt atgcctggcc
    18121 gcgagattat ggcacacttg tctggttaac tctcgtctca tacaggtaac acaaacgtga
    18181 aaatccttgt tgatgaaaaa tgaaaattat ttaacatttt gatataaaag gatttatatc
    18241 aattgttgtc cacgcaatga ccacattttc gagatataga aaaagcccgc agtttttacg
    18301 ctgcgggctt tgtttttctt tgtgcctttt gtgtgagggt gcgactttcg accgaacctt
    18361 tggcaaccgg atagcggggg ctttcacccc cgctgcggtt tcttacggtt tacactgcaa
    18421 gaacgccgca aattctgacc acatgatgtt aagtctgaac tcacacggag aacggtgtat
    18481 catccagaca gtcaacaaca tggttaagca taaaatgctc acgatgatcg ttttttgcgg
    18541 catagcgctt gactcctttt acagggaggc gctaaccttc cacttgctta gggtagatgg
    18601 tcagggcctc gggttaacat taaagttgac tccggggcct ttccacatcc ggccttcagg
    18661 taatccttcc agccatcagc cgaaaggcac ccgcgtgtaa tgtactgatt tccctttgtg
    18721 tgcgcaatgc ttgaggggga cggtaaaaaa acgcccgcat attgcgggca aatacgtctg
    18781 taatccaatg gagaaagggg tgccatatcg accgaacccg gcaaccggat agcgggggct
    18841 ttcacccccg ctgcggtttc ttactgttta cactgcaaga acgccgcaaa ttgtgaccac
    18901 atgatgttaa gcctgaactc acacggtgac ttgtgtatca tccagacaat catcaaaata
    18961 gttatgcata aaatgctcac tatgatcgtt ttttgcggca tagcgcttga ctccttttac
    19021 agggaggcgc taaccttcca cttgcgaggt agatgggtta gggcctcggg ttaaacaaat
    19081 gtttgactcg gggcctttcc acatccggcc ttcaggtaat ccttccagcc gtcagccgaa
    19141 aggcacccgc gcgtaattta ccttttacgt ccgttacgtg caacggatga cgtggctgtt
    19201 ttccttcccc ttcgccatcc tttttcggag cagttgtgca aaagtgtgca aaagtgattt
    19261 ttgctgccac tccccgccat gccgcgccag ttctggcttt cctgattttt tcgttttcgt
    19321 cgccggatcc tttttcagat cttcgatcgt gacgtaattt taaaaatctc ttgttattca
    19381 gtgcattaaa atttttcggg atcctcagaa agccggagtt tttgcacact tttgcaaaac
    19441 cttgcacaaa gtgcaaaacc attcagaagg gaaaacctca gtaatggcgc gccctggcgg
    19501 ggtgttttgc acccatttcg atttgcaaaa tttatcgtac acaaataccg gaggcgtgtg
    19561 cggggtagcg ccgattacgt cactttacgc gttacgtcgc ccccgaaaat ttccggccat
    19621 ttgcggcgat tttggggcgc agaaatcgtt ttctgatgtc aggggatcga atcgggctgg
    19681 cgggcttatc tcttcgttac gtggcgttac gcgacgtttt acgtggggat tacgtggggc
    19741 gtttcgtcca tctttttttc tgctgcggac ggttttttcg tggcgggtga tgtccggtga
    19801 tgaatatttt ttattatcgg ggggataaaa aaggccacat tgcgcggcct gttaaaatga
    19861 ataactatt
//
LOCUS       DAZKHK010000099        19633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKHK010000099 DAZKHK010000000
VERSION     DAZKHK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.099"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..844
                     /locus_tag="ACHOS5_003593"
     CDS             17..844
                     /locus_tag="ACHOS5_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9778351.1"
                     /translation="MLLIPCIGNAQEIKIDENWLHQSLNVIGRTDSRFGPRLTNDLYP
                     EYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNL
                     AFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNY
                     GAANENEWDGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIA
                     SSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1062..3494)
                     /gene="metL"
                     /locus_tag="ACHOS5_003594"
     CDS             complement(1062..3494)
                     /gene="metL"
                     /locus_tag="ACHOS5_003594"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP9778352.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLEHLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3497..4657)
                     /gene="metB"
                     /locus_tag="ACHOS5_003595"
     CDS             complement(3497..4657)
                     /gene="metB"
                     /locus_tag="ACHOS5_003595"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9778353.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4934..5251
                     /gene="metJ"
                     /locus_tag="ACHOS5_003596"
     CDS             4934..5251
                     /gene="metJ"
                     /locus_tag="ACHOS5_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP9778354.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5311..5919
                     /gene="yiiX"
                     /locus_tag="ACHOS5_003597"
     CDS             5311..5919
                     /gene="yiiX"
                     /locus_tag="ACHOS5_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP9778355.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5980..6192)
                     /gene="rpmE"
                     /locus_tag="ACHOS5_003598"
     CDS             complement(5980..6192)
                     /gene="rpmE"
                     /locus_tag="ACHOS5_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP9778356.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6395..8593
                     /gene="priA"
                     /locus_tag="ACHOS5_003599"
     CDS             6395..8593
                     /gene="priA"
                     /locus_tag="ACHOS5_003599"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9778357.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPIPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8749..9774
                     /gene="cytR"
                     /locus_tag="ACHOS5_003600"
     CDS             8749..9774
                     /gene="cytR"
                     /locus_tag="ACHOS5_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP9778358.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9866..10825
                     /gene="ftsN"
                     /locus_tag="ACHOS5_003601"
     CDS             9866..10825
                     /gene="ftsN"
                     /locus_tag="ACHOS5_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP9778359.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLVQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPAYTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10918..11448
                     /gene="hslV"
                     /locus_tag="ACHOS5_003602"
     CDS             10918..11448
                     /gene="hslV"
                     /locus_tag="ACHOS5_003602"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP9778360.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11458..12789
                     /gene="hslU"
                     /locus_tag="ACHOS5_003603"
     CDS             11458..12789
                     /gene="hslU"
                     /locus_tag="ACHOS5_003603"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP9778361.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12856..13782
                     /gene="menA"
                     /locus_tag="ACHOS5_003604"
     CDS             12856..13782
                     /gene="menA"
                     /locus_tag="ACHOS5_003604"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9778362.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13875..14360
                     /gene="rraA"
                     /locus_tag="ACHOS5_003605"
     CDS             13875..14360
                     /gene="rraA"
                     /locus_tag="ACHOS5_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP9778363.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(14445..14690)
                     /gene="zapB"
                     /locus_tag="ACHOS5_003606"
     CDS             complement(14445..14690)
                     /gene="zapB"
                     /locus_tag="ACHOS5_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP9778364.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15115..15960
                     /gene="glpF"
                     /locus_tag="ACHOS5_003607"
     CDS             15115..15960
                     /gene="glpF"
                     /locus_tag="ACHOS5_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP9778365.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15983..17491
                     /gene="glpK"
                     /locus_tag="ACHOS5_003608"
     CDS             15983..17491
                     /gene="glpK"
                     /locus_tag="ACHOS5_003608"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9778366.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGMEEYIRNNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17662..18672
                     /gene="glpX"
                     /locus_tag="ACHOS5_003609"
     CDS             17662..18672
                     /gene="glpX"
                     /locus_tag="ACHOS5_003609"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9778367.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18769..19515
                     /gene="fpr"
                     /locus_tag="ACHOS5_003610"
     CDS             18769..19515
                     /gene="fpr"
                     /locus_tag="ACHOS5_003610"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP9778368.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELERAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19520..>19633)
                     /locus_tag="ACHOS5_003611"
     CDS             complement(19520..>19633)
                     /locus_tag="ACHOS5_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="with UspC and Usp E is involved in resistance to UV
                     radiation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9778369.1"
                     /translation="LLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
BASE COUNT         4745 a         5135 c         5236 g         4517 t
ORIGIN      
        1 ctggatagct ttatccattc tattaatacc ttgcattgga aacgctcagg aaattaaaat
       61 tgatgaaaac tggttacatc aaagcttgaa tgtcattggt cgcacagact ctcgctttgg
      121 cccaagactg actaacgacc tctaccctga atatactgta gcaggaagaa aagactggtt
      181 tgatttttat ggttatgttg atctaccaaa attctttggc gtcggcagtc actatgatgt
      241 tgggatctgg gatgagggct caccactatt tacggaaata gaacctcggt tttccattga
      301 caaattgacc ggattaaatc ttgcgttcgg cccatttaaa gaatggttca ttgcaaacaa
      361 ctatgtctat gatatgggtg acaaccagtc atcccggcaa agtacatggt atatggggct
      421 tggtacagat atcgacacgg gtctaccaat taagctttct gccaatatat acgccaagta
      481 tcagtggcaa aactatggtg ccgctaatga aaatgaatgg gacggatatc gattcaaaat
      541 aaaatatagc atccctctta caaatttatt cggagggcga ttggtataca atagttttac
      601 taactttgat tttggctccg atcttgcgga caagtcacac aataataaac gaaccagtaa
      661 tgctattgct tcaagccata tcctttccct tctatatgaa cactggaaat ttgcatttac
      721 actacgttat tttcacaacg gtggacaatg gaatgcggga gagaaggtta acttcggaga
      781 tggtccattt gaattgaaaa atacaggatg gggaacctat actactattg gttatcaatt
      841 ttaaattagg tgacatttta gatattgtta cttaatgtca ttttgtaatt gtcattaata
      901 acttcaccat tcactctgtt acttcatcgc tcaacacaaa agccccctta ttaccggggg
      961 cactgaccaa aggcaaaatg acaatcaatc accacttccc caggaaaagg tttaggggtg
     1021 acaacaaaag ctggcaattg ccagcttttg ttcaataatc actacaacaa ctgcgccagc
     1081 cggttaatat ccgactgaat cgccccggcg gtgacgtcgc gccctgcacc tggcccgcgg
     1141 atcaccagcg ggttgtcgcg ataccagcgg ctttcgatgg caaagacgtt atcgcatggc
     1201 agtagtgatg ccagcggatg gtcttcacgc accgcttcca cgcccacacg cgctttgccg
     1261 ttggcatcga aacgcgccac gtagcgcagc accagcccca tttcgcgggc cgcttccagt
     1321 cgttgcacca tctgctcgtt aagttcatcg ccgttttcaa agaaatggtc gatgctaccg
     1381 ccttcgcaat gggcaggcac cagcgattcc acgcgcacct gatctggttc gatgtcgtaa
     1441 cccgcttcac gcgccagaat caccagcttg cgcatcacgt ctttgccgga aaggtcgtca
     1501 cgcgggtcag gctcggttaa cccttgctgc cacgcctgat ccaccagctc ggtaaacggc
     1561 acgctaccgt cgaattgcag gaacagccag gagagcgtgc cggagaagat cccactgatc
     1621 gacaaaatag tatcgccgct gtcgatcaga tcgcgcacgg tatggttgat cggcaaaccc
     1681 gcaccgacag tggcattgta cagccagtga cgcccggttt tttcgaaggc gtcgtggatc
     1741 tggcgatatt tattgctgtc gctggctcct gccagtttgt tggcactgat gacgtggaaa
     1801 ccgtggctgg cgaaatcaag atactggtca gcgagctgct ggctggcggt aacgtccagc
     1861 accactaaat catcatacgg gtgggcgcgc atccacagga acagcgactc ttcatcctgc
     1921 tcgaccgctt catcgttgaa gaaggctaac gcgcggctgg cgtccagccc gtcatagctc
     1981 aacaggctgc ggcggctgtc caccacgcct gccagcacaa attcaaagcc ggtacgtgcc
     2041 gaaagcgtac tctgctcacg ggcgaacagt tccagccagc gagaaccgat attgcccttg
     2101 ccgaacaaca ccaggccgat gcgtttttcc gcacggaaga cggactgatg cagcccctga
     2161 atcaggcttt cggtcgggcc ggtgcgcagt actgccacca ggctgatgcc gtcatcggac
     2221 tgccaggtaa attcgaccgg ctggcctttc agttgctgcc agaagcggtg gcaatgcagc
     2281 gggttacggg tgacgcccgc cccaaccatc gccaccagcg ctagcccctg acgcaggcgc
     2341 agttcgccag gtaatcccgc ttcgtcgagg attttcagcg cactgtcggc cacttctgag
     2401 gtgtagcaaa attgcagcaa ctggcgatcg ttatgcacgc caaccgccag tgggcgcacc
     2461 tgagcacgtt tcaggatctg gtcgatctct ttatgcgcca gtttgaaatc ctggctggcg
     2521 ggcacctgaa actcaatcaa gcagacatca tcatggctgg tgacaatacg tgcaccagta
     2581 ccggaggcca gcacgcgttc aatgcgcgtg gaaccttgat ccggcgtgta gctacagcgc
     2641 agttgcaagt cgatttcgct gccagaaacg ggctgtaaag tacgggcatg aagaacggga
     2701 gccgccagac gcgccagttc gctggcttca tccagacgca gcaacggcag caggcaggca
     2761 tctttcactt tacgcgggtc ggcgctatat accccggcga cgtcgctcca gatggttacg
     2821 cgagaaacac ccgccagcgc accgatttgt gtcgcggaat agtcggaacc gttacgcccc
     2881 agcagcaccg tttcaccggc gttgttacgg ctgataaacc cggtcaccac cagacgtttg
     2941 cccggatgtt gtaccagtag ctgttgcagc aacgggtacg aaagcccttc atcaacctgc
     3001 ggttgcgcag cgcgttcagc gcgtaaaaac tcgcgggcat caagccaggc cgctggcagc
     3061 ccttgttgat taagcaccgc agacatcagg cgcgccgacc acacttcacc gtggcccacc
     3121 acttccgcat acaccgcatc gttaataccg ctgtcgagca acgccgccag gtgctcaaga
     3181 tcgctgacaa aagcgctaat gaggttatcg gcttcttcgg cgggtagcag gccgctaatc
     3241 agatcgcact gataacgacg taatgtttgt tgaacctgat gcgcagagag acgatcggtc
     3301 tggcttagtt tcaaccagtt aatcaactgg ttagtggtgc taccggcggc ggaaaccacc
     3361 atcatatcgt caggctgaga gtactccgcc ataatgcccg cgacacgcaa ataacacttc
     3421 acatcagcca gactactgcc accaaattta tgcagttgac gacctttcgc ccctgcctgc
     3481 gcaatcacac tcatttttac cccttgtttg cagcccggaa gccattttcc aggtcggcaa
     3541 ttaaatcttc gccatcttca ataccggtgg agatacgcag cagcgtctcg gagatcccgg
     3601 cggcagcacg cgcttctggt gccatgcctg catgtgtcat ggttgcggcg tgagagatta
     3661 aactttccac tccccctaat gattccgcca gcgtaaacaa cgacagcccg cccaggaaac
     3721 gacgcagcgt ctgctcatcg ccatccagtt caaaactcaa cattgcgcca aagccttttt
     3781 gctggcgcgc ggcaatttca tgcccctgat tttccggcag cgacgggtga tacagttttt
     3841 tcaccaacgg ctgggtttgc agatatttca caatcgcctg cgcgttgcgc tgggccagct
     3901 ccattcgcgg caccagcgtt cgcaacccgc gcagcagcag atagctgtca aacgcgccgc
     3961 ccgtcacgcc aatattgttt gcccaccagg caagttcagt gacaacgtcc gggtctttag
     4021 caatcactac gcctgccacc acgtcagagt gaccgttcag atatttcgtg catgaatgca
     4081 acaccagatc ggcacctaat gccagtggat tttgtaatgc cgggcttaag aaggtgttat
     4141 ccaccacgct caccgccccg acttcccttg ccagatggca gattttcgca atatccacga
     4201 cgcgtaacaa tggattactt gggctttcta ccagtaccag tttggggttt tctgccagcg
     4261 ctgcccgtaa tgcctgttca tcgctttgat caacaaacaa cacgcgatag caaccgcgtt
     4321 tcgccaggct gtcgaacagg cgatagctac cgccgtagca gtcgtgcggc gcaaccagca
     4381 gatcgccagg tttcaaaaag acggtcgtta ccaggtgaat cgcggacatg ccggtattag
     4441 taagtactgc accagcaccg ccttccagtt ctgccagcgc tcgctgaacc acatcgcgcg
     4501 ttgggttgcc gcgccgcgag taatcatgcg cgcgcggttc attaaatccg gtaaagttat
     4561 aggtactgga aagatggatg ggagggacaa cgcaaccata ctgttcgtcg tcatttaacc
     4621 cgctacgcac tgcgatggtg gcctgtttac gcgtcatgtg atgaagttcc ctgggcttgg
     4681 tcggtgaaat gtcaggcacc agagtaaaca ttgtgttaat ggacgtcaat acatctggac
     4741 atctaaactt ctttgcgtat agattgagca aatctcaaat agccgttaaa attatatgca
     4801 ttatcacgcc gacaggtgca ttacacgatg tcacggtaac gcctgtacgg taaactatgc
     4861 gggtttacgg tcagtaccca tattaattgt gtggtctggc ctcaatttat tgacgaagag
     4921 gattaagtat ctcatggctg aatggagcgg cgaatatatc agcccatacg ctgagcacgg
     4981 caagaagagt gaacaagtca aaaaaattac ggtttccatt cctcttaagg tgttaaaaat
     5041 cctcaccgat gaacgcacgc gtcgtcaggt gaacaacctg cgtcacgcga ccaacagcga
     5101 gctgctgtgc gaagcgtttc tgcatgcctt taccgggcaa cctttgccgg atgatgccga
     5161 cctgcgtaaa gagcgcagcg acgaaattcc ggaggcggca aaagagatca tgcgtgagat
     5221 ggggattaac ccggagacgt gggaatacta aagcgaaaaa gagcggcgcg gagttaacca
     5281 gccgcttttt tcatacatgg acgtttaacg atgaaaatca ggctactgat tctcagcctg
     5341 ctggtttctg tgcctgcatt tgcctggcaa ccacaaaccg gcgacatcat ctttcagatc
     5401 tctcgctcat cgcaaagtaa agcgatccaa ctggcgaccc attccgatta cagccacacc
     5461 ggtatgctgg tgatacgcaa caaaaagccc tacgtttttg aagcggtcgg cccggtaaaa
     5521 tacaccccac tcaagcagtg gatcgcccat ggtgaaaagg gcaaatacgt tgttcgccgc
     5581 gttgaaggcg gactgagtgt tgaacaacag caaaaactgg cgcaaacggc aaaacgttat
     5641 ctcggtaaac cgtatgactc cagcttctcg tggagcgacg atcgccagta ctgttcggaa
     5701 gtggtgtgga aggtctacca gaatgcgctg ggaatgcgtg tgggcgagca gcagaagctg
     5761 aaagagttcg atcttagtaa cccgctggtt caggcaaagc tcaaagaacg ctacggcaaa
     5821 aatattccgc ttgaggaaac ggtagtttca ccacaagccg ttttcgatgc gccacaactc
     5881 accacggtcg ccaaagaatg gccgctgttt tcctggtaat acgaccaaaa ttcaggcaca
     5941 aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc ccgggatgtt
     6001 gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct gtttgccagt
     6061 gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa cggtggagcg
     6121 gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt tcgggtgaat
     6181 atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca
     6241 gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga aggcgcgaaa
     6301 tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg caatgtgtat
     6361 actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg ttgccttgcc
     6421 cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatgacgg ttaaagccgg
     6481 gagtcgcgtg cgcgtgccgt ttggcaaaca acaggagcgt atcggtgttg tggtgtcagt
     6541 gagcgatgtc agtgagctgc cgcttaacga gctgaaagcg gtagttgaag tgctggacgt
     6601 tgagccggta tttactcact ctgtctggcg attactgctc tgggcggcag attactatca
     6661 tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc
     6721 tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggtcagg cggtggatct
     6781 gaacagcctg aaacgttcgc caaagcaaca acaggcgctg gcagcgttac ggcaaggcaa
     6841 aatctggcgt gatcaggtcg cggaacttga atttaatgat gccgcgttgc aggcactgcg
     6901 caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact ggcgaacgaa
     6961 ctatgccgtt tctggtgagc ggttgcggtt gaataccgaa caggccaccg ccgttggcgc
     7021 aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta ccggttccgg
     7081 taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca aacaggcgct
     7141 ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc gtgaacgttt
     7201 taatgccccg gtggaagttc tgcattccgg cctgaacgac agcgagcgtc tttcggcgtg
     7261 gttgaaagcg aaaaatggcg aggcggcgat tgtcatcggc acccgctccg cgctgtttac
     7321 gccgtttaaa aatctcggcg tgattgttat tgatgaagag cacgacagct cctacaagca
     7381 gcaggaaggc tggcgctatc atgcccgcga cctggcggtg tatcgcgcgc atagcgagca
     7441 aatcccaatt attcttggct ccgcaacgcc cgcactggaa acgttatgca acgtgcagca
     7501 gaaaaaatac cgcctgctgc gcctgacccg tcgggcgggc aatgcgcgcc cggcaattca
     7561 acatgtgctg gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg cgttaatcac
     7621 ccgtatgcgc cagcatttac aggccgacaa ccaggttatc ctctttctta accgccgggg
     7681 ttttgcgcct gcgctactgt gccacgactg tggttggata gccgaatgcc cacgttgcga
     7741 tcactactac acgctgcatc aggcgcaaca gcatttgcgc tgccaccatt gcgacagtca
     7801 gcgtccggtg ccgcgccagt gcccttcttg cggttccacg cacctggtac ccgtggggct
     7861 gggcaccgaa cagcttgaac agacgctcgc gccgttgttc cctggtgtcc ccatttctcg
     7921 tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg cggaagtgca
     7981 tcgcggcggc gcgaggatct tgattggcac gcaaatgctg gcgaaaggtc accactttcc
     8041 ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg ctgttttctg ccgattttcg
     8101 ctcggcagaa cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg ggcgtgcggg
     8161 taaacagggc gaagtggtgc tgcaaacgca ccatccggaa catcctctgt tgcaaacatt
     8221 gctctataaa ggctacgacg cctttgccga acaggcactg gctgaacgac gcatgatgca
     8281 gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca atcagcacgc
     8341 gccattgttc ctgcaacaac tgcgtaatct cattctcgcc agcccactgg cagacgagaa
     8401 actgtgggtt ctcggtccga ttccggcgct ggcccctaaa cgtggcggtc gctggcgctg
     8461 gcagatattg ttgcagcacc cttcccgcgt gcgcttacag cacatcatca atggtacgct
     8521 ggcgcttatt aatacaatac cggattcccg caaggtgaaa tgggtgctgg atgtcgatcc
     8581 gattgagggt taaaccgctc acgatgcgag gcggatcgaa aaattcaata ttcatcacac
     8641 ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg
     8701 aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt gaaagcgaag
     8761 aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt ctctacagcg
     8821 accgtctccc gagcattaat gaatcccgat aaagtctccc aggccactcg taatcgggtt
     8881 gaaaaagcgg cccgggaagt gggttattta ccacagccta tggggcgcaa cgtcaagcgt
     8941 aatgaatccc gtaccattct ggtgattgtc ccggatatct gcgatccctt ctttagcgaa
     9001 attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct gattggcgac
     9061 tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt
     9121 gatggcatgt tgttgctggg ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt
     9181 aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg aactggagct gcctaccgtt
     9241 catatcgaca atctgaccgc cgcatttgat gcagtaaatt atttatatga gcaagggcat
     9301 aatcggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta ccgcctgcaa
     9361 ggctatgtcc aggcgctgcg tcgctgcggc attatggttg atccgcaata catcgcccgt
     9421 ggcgacttca ccttcgaagc tggaagcaaa gcaatgcaac agttgcttga tcttccacaa
     9481 ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact ttctcaggca
     9541 aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga taacatcgac
     9601 ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta cgaaatcggt
     9661 cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg cagtggctct
     9721 cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa
     9781 cctttaagac tgccgtgact ggtcaaaggc ccgcagctta agtaacatgg cgggctgacg
     9841 aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc gcagccaacc
     9901 ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc tgcctgcggt
     9961 ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg gtggtctgta
    10021 cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga aagtgaccgg
    10081 aaacggacta ccaccaaaac cagaagaacg ctggcgctac atcaaagagc tggaaagtcg
    10141 ccagccgggt gtgcgtgcgc ctacagaacc ttctgccggt ggtgaagtga aaacgccgga
    10201 gcaactgaca ccagaacaac gccagcttct tgagcaaatg caggctgata tgcgccagca
    10261 gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc gtcagcaaac
    10321 gctacagcgc cagcgtcagg cgcagcaatt agcggagcag caacgcctgg ttcagcagtc
    10381 gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca
    10441 ggcgcaaccg cgccagtcta aaccggctta tactcagcag ccgtaccagg atctgctgca
    10501 aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa gctgcgccgg ttgctcgtgc
    10561 cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg
    10621 cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg cgttcgaagg
    10681 ctttgactca aaaattacca ccaacaatgg ctggaatcgt gtggtgattg gcccggtgaa
    10741 aggcaaagag aacgcagaca gcaccctcaa tcgcttgaag atggcgggtc atacaaactg
    10801 cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat aattgcatta
    10861 tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg
    10921 acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga tggtcaggcc
    10981 acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac
    11041 aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt cgaactgttt
    11101 gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga gctggcgaaa
    11161 gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa
    11221 actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa cgatcttatt
    11281 gctatcggct ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt agaaaacact
    11341 gaacttagcg cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga catttgcatc
    11401 tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg atctcccatg
    11461 tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat cggccaggac
    11521 aacgccaagc gttccgtggc gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac
    11581 gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc gaccggtgtc
    11641 ggtaaaactg aaatcgcccg tcgcctggct aagctggcga atgcgccgtt catcaaagtt
    11701 gaagcgacca aattcaccga agtgggctat gtcggtaagg aagtggattc tattattcgc
    11761 gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat
    11821 cgcgctgaag aactggcaga agaacgtatt ctcgacgtac tgatcccacc ggctaaaaat
    11881 aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc attccgcaaa
    11941 aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc cgcagcaccg
    12001 atgggcgttg aaattatggc tcctccgggc atggaagaga tgaccagcca gctgcagtcc
    12061 atgttccaga acctgggcgg tcagaaacaa aaagcgcgta agctgaaaat caaagacgcc
    12121 atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga gctgaagcaa
    12181 gatgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc
    12241 tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca gcgtgacctg
    12301 ctgccgctgg tagaaggttg cactgtttcc accaaacatg ggatggtcaa aactgaccac
    12361 attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct gatcccggaa
    12421 ctgcaaggtc gtctgccaat ccgcgttgag ctgcaggcac tgaccaccag cgacttcgag
    12481 cgtattctga ccgagccgaa tgcctctatc actgtgcagt acaaagcact gatggcaact
    12541 gaaggcgtaa atatcgagtt taccgactcc ggtattaagc gcatcgcgga agcggcatgg
    12601 caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacataccgt tctggagcgt
    12661 ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaactat cactattgac
    12721 gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct gagccgtttt
    12781 atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa ggccccattt
    12841 ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt
    12901 ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac ggcgctggca
    12961 tggtggcaag gtcacttcga tccactggtc gccctgctgg cattgattac cgccgggcta
    13021 ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg cagcgataaa
    13081 cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca gcaagagatg
    13141 aaacgggcgc tcattattac cgttgtgctc atctgtcttt ccgggctggc actggttgca
    13201 gtggcgtgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg gttgtcgatc
    13261 attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg tttgggtgat
    13321 atttccgtac tggttttctt tggctggttg agcgtcatgg ggagctggta tttacaggct
    13381 catacattga ttccggcact gatccttccg gcaaccgcat gcggcctgct ggcaacggca
    13441 gtactgaaca ttaataacct gcgtgatatc aatagcgacc gcgaaaacgg caaaaacacg
    13501 ctggtggtgc gcttaggcgc agtaaacgcg cgtcgttatc atgcctgcct gctgatgggc
    13561 tcactggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg gggctggctg
    13621 ttcctgctgg cggcaccgtt gctggtgaag caagctcgtt atgtgatgcg cgaaatggac
    13681 ccggtggcga tgcgaccaat gctggaacgc actgtcaaag gagcgttact gactaacctg
    13741 ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa tatcaattaa
    13801 caattgatga ttttgccaac agcccacata gcgcgatata ctgaaaattc tcgcagcaac
    13861 tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat caagaagatg
    13921 ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa
    13981 taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc gaacagaatg
    14041 gccgtggtcg tgttcttgtc gtcgatggcg gaggttctgt tcgtcgcgca ctggtcgatg
    14101 ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt tacggcgcgg
    14161 tgcgtcaggt agatgacctg gaagagttgg atatcggcat ccaggcgatg gcggcaattc
    14221 cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg
    14281 tcaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt ctttcagaag
    14341 atccgctgga tattgaatga taaaaaaggc accgcaaggt gccttttttc tgcgttacct
    14401 gttggcctac acagtaaaga aattacgcga aagatgaagt gtaatcagac ctcttccatg
    14461 cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt cagatggttg
    14521 ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg cgacagtgag
    14581 ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc
    14641 tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat acctctcctg
    14701 aattgcaagg cgttgatgga taaaaatcct cgtcccgatt accggtgacg ccttaataaa
    14761 tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg tccagcggca
    14821 cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca taatccacac
    14881 caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc atcatgtaca
    14941 atcagatgga ataaatggcg cgataacgct cattttatga cgaagcacac acattttaag
    15001 ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta caagcatcgt
    15061 ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc gattatgagt
    15121 caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg gttgttgatt
    15181 tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt tggtcagtgg
    15241 gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac cgcaggggtt
    15301 tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc ctgtttcgac
    15361 aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg tgctgcggct
    15421 ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca tcacattgtt
    15481 cgcggcagcg ttgaaagtgt tgatctggct ggcactttct ctacttaccc taatcctcat
    15541 atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct gatggggctg
    15601 atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc tcccttgctg
    15661 attggtctac tgattgcggt cattggcgca tctatgggcc cattgacggg ctttgccatg
    15721 aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg gggcaatgtc
    15781 gcctttaccg gcggcagaga cattccttac ttcctggtgc cgctttttgg ccctatcgtt
    15841 ggcgcgattg taggtgcatt tgcctatcgc aaactgattg gtcgccattt gccttgcgat
    15901 atctgtgttg tggaagaaaa ggagaccaca actccttcag aacaaaaagc ttcgctgtaa
    15961 tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc tcgaccaggg
    16021 caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta gcgtgtcgca
    16081 gcgcgaattt gagcaaatct acccaaaacc aggctgggta gaacacgacc caatggaaat
    16141 ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata tcagttccga
    16201 tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct gggaaaaaga
    16261 aaccggcaaa cctatctata acgccattgt ctggcagtgc cgtcgtaccg ccgagatctg
    16321 cgaacatctc aaacgcgatg gcatggaaga gtacattcgt aacaacaccg gtctggtgat
    16381 tgacccgtac ttctctggca ccaaagtgaa gtggatcctc gaccatgtgg aaggctctcg
    16441 cgagcgtgcg cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc ttatctggaa
    16501 aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta ccatgttgtt
    16561 taacatccat actctggact gggacgacaa aatgctggag gtgctggata ttccacgcga
    16621 gatgctgcca gaagtgcgtc gttcttccga agtatacggt cagactaaca ttggcggcaa
    16681 aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggcgg cgctgtttgg
    16741 tcagttgtgc gtgaaagaag gaatggcgaa gaacacctat ggcactggct gctttatgct
    16801 gatgaacact ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca ccatcgcctg
    16861 cggcccgact ggcgaagtga actatgcgct ggaaggtgca gtgtttatgg cgggcgcgtc
    16921 cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt ccgaatattt
    16981 cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggccttta ccgggctggg
    17041 tgcgccatac tgggatccgt atgcacgcgg ggcgattttc ggtctgactc gtggggtgaa
    17101 cgctaaccac attatacgcg cgacgctgga gtctattgct tatcagacgc gtgacgtgct
    17161 ggaagcaatg caggccgact ctggtattcg actgcacgcc ctgcgcgtgg atggcggcgc
    17221 agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggtacgc gcgttgagcg
    17281 cccggaagtg cgcgaagtca ccgctttggg tgcggcctat cttgctggtc tggcggttgg
    17341 cttctggcag aatcttgacg agctgcaaga gaaagcggtg attgaacgcg agttccgtcc
    17401 tggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag cagtcaaacg
    17461 cgcgatggca tgggaagaac acgacgagta ataatgatcg cctgatgcga cgcttgcgcg
    17521 tcttatcagg cctacgagtc ggatacacat ccggcatcac acagactctg aaaccgtgcg
    17581 ggggcaaccc cgcacacatc aataatcccg cccttcccct gtgctacact tcgcgccatt
    17641 ccttactgct tagagtttgc tatgagacga gaacttgcca tcgaattttc ccgcgtcacc
    17701 gaatcagcgg cgctggctgg ctacaaatgg ttaggacgcg gcgataaaaa caccgcggac
    17761 ggcgcggcgg taaacgccat gcgtattatg ctcaaccagg tcaacattga cggcaccatc
    17821 gtcattggtg aaggtgaaat cgacgaagca ccgatgctct acattggtga aaaagtcggt
    17881 actggtcgcg gcgacgcggt agatattgct gttgatccga ttgaaggcac gcgcatgacg
    17941 gcgatgggcc aggctaacgc gctggcggtg ctggcagtag gcgataaagg ctgtttcctc
    18001 aacgcgccag atatgtatat ggagaagctg atcgtcggac cgggagccaa aggcaccatt
    18061 gatctgaacc tgccgctggc ggataacctg cgcaatgttg cggcggcgct cggcaaaccg
    18121 ttgagcgaac tgacggtaac gattctggca aaaccgcgcc acgatgccgt tatcgcagaa
    18181 atgcaacaac tcggcgtacg cgtatttgct attccggatg gcgatgtggc ggcctcaatt
    18241 ctcacctgta tgccggacag tgaagtcgat gtactgtacg gcattggcgg cgcgccggaa
    18301 ggcgtggttt ctgcggcggt gatccgcgca ttagatggtg acatgaacgg tcgtctgctg
    18361 gcgcgtcatg acgtcaaagg cgacaacgaa gagaatcgtc gcattggcga gcaggagctg
    18421 gcacgctgca aagcgatggg catcgaagcc ggtaaagtat tgcgtttggg cgatatggcg
    18481 cgcagcgaca acgtcatctt ctctgccacc ggcattacca aaggcgatct gctggaaggt
    18541 attagccgca aaggcaatat cgccaccacc gaaacgctgc tgatccgtgg taaatcacgc
    18601 accattcgtc gcattcagtc catccactat ctggatcgta aagacccgga aatgcaggtg
    18661 cacatcctct gattgatttg atcgattgag ccttccagtc cttcgggact ggaatttttt
    18721 tgttcggaga acgaagataa ggcaagtcaa tcaaaacagg agaaaaacat ggctgattgg
    18781 gtaacaggca aagtcactaa agtgcagaac tggaccgacg ccctgtttag tctcaccgtt
    18841 cacgctcccg tgcttccgtt taccgccggg caattcacca aacttggcct tgaaattgaa
    18901 ggcgaacgcg ttcagcgcgc ctactcctat gttaactcgc ccgataatcc ggatctggag
    18961 ttttacctgg tcaccgtccc cgatggcaaa ttaagcccgc gactggcggc actgaaaccg
    19021 ggcgatgaag tgcaggtggt tagcgaagcc gctggcttct ttgtgctgga tgaagtacca
    19081 gattgcgaaa cgctatggat gctggcaacc ggtacagcga ttggccctta tttatcgatt
    19141 ctgcaattgg gcaaagattt agaacgcttc aaaaacatgg tgctggttca cgccgcacga
    19201 tatgccgccg acttaagcta tctacctctg atgcaggaac tggaaaaacg ctacgagggg
    19261 aaactgcgca ttcagacggt ggtcagtcgg gaaacggctg cgggttcgct cactgggcga
    19321 ttaccggcgt taattgaaag cggtgaactg gaaagggcgg ttggcctgcc gatgaataaa
    19381 gaaaccagcc atgtgatgct gtgcggcaat ccacagatgg tgcgcgatac acaacagttg
    19441 ctgaaggaga cccggcagat gacgaaacat ttacgtcgtc gaccgggcca tatgacagcg
    19501 gagcattact ggtaagcggt tacttgtcga taaacggcac gatcaagaga tccgcactca
    19561 acttattgat catcccgcga tatgccggca tcaaacggtt gataaatgag tggtgatgac
    19621 cacagacaag gag
//
LOCUS       DAZKHK010000100        18772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKHK010000100 DAZKHK010000000
VERSION     DAZKHK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.100"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..1017
                     /locus_tag="ACHOS5_003612"
     CDS             46..1017
                     /locus_tag="ACHOS5_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFP9778370.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISNGARVTAQDVPITGGPYKNIVANYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKLMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1120..1623
                     /locus_tag="ACHOS5_003613"
     CDS             1120..1623
                     /locus_tag="ACHOS5_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP9778371.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1690..3147)
                     /gene="nuoN"
                     /locus_tag="ACHOS5_003614"
     CDS             complement(1690..3147)
                     /gene="nuoN"
                     /locus_tag="ACHOS5_003614"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP9778372.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3154..4683)
                     /gene="nuoM"
                     /locus_tag="ACHOS5_003615"
     CDS             complement(3154..4683)
                     /gene="nuoM"
                     /locus_tag="ACHOS5_003615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP9778373.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4847..6688)
                     /gene="nuoL"
                     /locus_tag="ACHOS5_003616"
     CDS             complement(4847..6688)
                     /gene="nuoL"
                     /locus_tag="ACHOS5_003616"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP9778374.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTEVAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6685..6987)
                     /gene="nuoK"
                     /locus_tag="ACHOS5_003617"
     CDS             complement(6685..6987)
                     /gene="nuoK"
                     /locus_tag="ACHOS5_003617"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP9778375.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6984..7538)
                     /gene="nuoJ"
                     /locus_tag="ACHOS5_003618"
     CDS             complement(6984..7538)
                     /gene="nuoJ"
                     /locus_tag="ACHOS5_003618"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP9778376.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7550..8092)
                     /gene="nuoI"
                     /locus_tag="ACHOS5_003619"
     CDS             complement(7550..8092)
                     /gene="nuoI"
                     /locus_tag="ACHOS5_003619"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP9778377.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8107..9084)
                     /gene="nuoH"
                     /locus_tag="ACHOS5_003620"
     CDS             complement(8107..9084)
                     /gene="nuoH"
                     /locus_tag="ACHOS5_003620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP9778378.1"
                     /translation="MSWITPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9081..11807)
                     /gene="nuoG"
                     /locus_tag="ACHOS5_003621"
     CDS             complement(9081..11807)
                     /gene="nuoG"
                     /locus_tag="ACHOS5_003621"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP9778379.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSQTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11860..13197)
                     /gene="nuoF"
                     /locus_tag="ACHOS5_003622"
     CDS             complement(11860..13197)
                     /gene="nuoF"
                     /locus_tag="ACHOS5_003622"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP9778380.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13194..13694)
                     /gene="nuoE"
                     /locus_tag="ACHOS5_003623"
     CDS             complement(13194..13694)
                     /gene="nuoE"
                     /locus_tag="ACHOS5_003623"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP9778381.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13697..15499)
                     /gene="nuoC"
                     /locus_tag="ACHOS5_003624"
     CDS             complement(13697..15499)
                     /gene="nuoC"
                     /locus_tag="ACHOS5_003624"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP9778382.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15593..16255)
                     /gene="nuoB"
                     /locus_tag="ACHOS5_003625"
     CDS             complement(15593..16255)
                     /gene="nuoB"
                     /locus_tag="ACHOS5_003625"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP9778383.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16271..16714)
                     /gene="nuoA"
                     /locus_tag="ACHOS5_003626"
     CDS             complement(16271..16714)
                     /gene="nuoA"
                     /locus_tag="ACHOS5_003626"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP9778384.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17344..18282)
                     /gene="lrhA"
                     /locus_tag="ACHOS5_003627"
     CDS             complement(17344..18282)
                     /gene="lrhA"
                     /locus_tag="ACHOS5_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP9778385.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4711 a         5176 c         4823 g         4062 t
ORIGIN      
        1 ataacaaata atgattcaac aacgcaaccc attaataatt tgcatatgaa aaaaataaat
       61 tttgctttta ttatattatt tctgttttca ctccctctga ttatttttta ccaaccctgg
      121 gtgaatgcat taccgccaac gccccgacac gcaagtcctg agcaattaga aaaaacagtt
      181 cgttatctta cacaaacagt gcatccacgt agcgccgaca atattgataa tctgaatagg
      241 tccgcagagt atataaaaga ggtctttatc agtaacggtg ccagagttac cgcgcaggat
      301 gtccccatta cgggtggccc ctacaaaaac attgttgcta attatggtcc tgccgatgga
      361 ccgctgatta ttattggtgc gcactatgac tctgtcagta gttatgaaaa cgatcaattg
      421 acctatacgc cgggcgcgga tgataacgcc agcggcgtgg caggattact cgaactggca
      481 cgtttgttac agcagcagac tccagggatt ggcgtccaac tggtcgccta tgcgtcggaa
      541 gaaccgccct tctttcgtag cgatgaaatg gggagcgcgg tacatgcagc ttcgcttgag
      601 cgtccagtga aattaatgat agcgctggag atgattggtt attacgactc tacgcctggt
      661 agccaggatt acccatcccc ggcaatgtcc tggctttatc ccgatcgggg agactttatt
      721 gccgtggtcg gcagaatgca ggatatcaac gccgttcgtc aggtaaaagc ggcattgttg
      781 tcatcccggg atttatctgt ttattctatg aatgccccag gttttattcc cggtattgat
      841 ttctctgatc accttaatta ttggcaacac gatattcccg ccgtaatgat tactgacacc
      901 gctttttatc gtaataaaca ataccacttg cccggtgata tcgcagacag attaaattat
      961 cagaaaatgg ctcaggtagt ggatggagtt acaaccttgt tatacaacag taaataataa
     1021 aaatttctcg aggcggatcc agaaaaagca acatgcgcat tttattttct ttcgtctgtt
     1081 ttaagatgcg aaacatgaaa ttcaacggag gaaaaataaa tgggaatgat tggctatttt
     1141 gcagaaattg actcagaaaa gattaaccaa ctgcttgagt ctactaaaaa acctttgatg
     1201 gataatatac atgacacgct ttcaggatta cgccgactgg atattgataa acgctgggat
     1261 tttttacact ttgggctgac aggcacctct gcttttgatc ccgcgaagaa tgacccgctg
     1321 agtcgcgccg tgcttggtga acacagccta gaagatggca tagatggctt cctgggattg
     1381 acctggaatc aggagttagc cgcgacaatc gaccgactgg aatcactgga tcgaagtgag
     1441 ctgcgtaaac agttttccat taagcgactc aatgaaatgg agatttatcc gggagtaacc
     1501 ttttcagaag agttagaggg gcaactcttt gcttcgatca tgcttgatat ggaaaagctg
     1561 attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt tattgttggg
     1621 tgatggtgtc agggcaccaa aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa
     1681 actgactgtt tacatcagcg gcattgctaa ccgcacaatg ctaatcagcg gttgtggcca
     1741 tacacccagc accagtacca acagtgcaga gatcagcacc acgataccgc ctgcgctgta
     1801 ctgccagttt gatggtgcat cgcgacccgg ttgttccgga gcgtgcaggt ataagctcac
     1861 cgccacgcgc aggtagtagt aaaggccgat tgccgaacca acaaccacgg cacccaccag
     1921 ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga taaagcccag
     1981 cgtcattggg ataccagcca gtgaaagcat catcaccgtc atcactgccg cgaggatcgg
     2041 acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg
     2101 gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccgaccag
     2161 gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca gcgccaccag
     2221 cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatat tggtctggct
     2281 cagcgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca gcactacgcg
     2341 aatcgcttca ctgtcaccca ccggtgcgta aaggaacaaa cgcatcacca cgccgaagat
     2401 agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac
     2461 gtctggcgtc cacaggtgga acggcaccag agagagtttg aaaccgaggc caacaatcat
     2521 caggccaaaa cctgccagca acagcggctc gttgagcata ccgtcgccga ggtttttacc
     2581 cagtgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac cgaacagcag
     2641 gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca gcgaacgttt
     2701 ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt cgataccgag
     2761 gaacagagac gccagatggt tggcattcgc cagcaggatc ccgcccagcg cggcaattaa
     2821 caccaacagg tagaactcgt ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa
     2881 agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc
     2941 aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccgacaaacc agagcgatac
     3001 cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca
     3061 cgcaatggag agcatcacaa ccaccaccgt caagccgacg atcagcaacg gtagcagtgc
     3121 gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt
     3181 aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg
     3241 atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag
     3301 cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga aataagcgcg
     3361 atgcagcatc gccagcgaat aaacagaagc aaagaccagc ccaaaggtag agataacggt
     3421 aatcaccggg acaacctgga agctgccgaa caaaatcata aattcgccga cgaagttacc
     3481 ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacagtgctg gcagccattt
     3541 catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag
     3601 ctgaccacaa agaataaaca gacccgccgc cgacaagccg tgcgcaatca tctggattac
     3661 cgcgccctgg taggccaact ggctgccagt gtagatagca atcagcacga agcccatgtg
     3721 ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc
     3781 accatagaag atgccgataa cacccagcca catggcgatt ggcgcaaact ctgccgacgc
     3841 gttcgggaac agcggcaagg agaaacgcaa cagaccgtaa gcggcggttt tcagcaagat
     3901 ccccgcgagg tcaacggaac cggcggtcgg tgcctgggag tgcgcatccg gtaaccagcc
     3961 atgcagcgga accaccggca ttttcaccgc gaaggcgatg aagaagccca gcatcaacag
     4021 atattccacg ccgttagaca tcggcgtatt cagcagctct tcatagttga aggtccagac
     4081 gccggtcgca ttgtagtgaa cgaaaaccag cgccaggatg gcgatcaaca tcaccagacc
     4141 actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga
     4201 ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa
     4261 gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca
     4321 catcaggttg aggtggaaga agccctgata tttttcgatc tctttccatg aacagagtac
     4381 cgccagcaca ccgagcagac cggtcagcac gaccatcagc aacgacagac cgtcaatggc
     4441 gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg
     4501 cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag
     4561 cgccagcgtc aatcccatgg ttaccagcgc gatccagcgc ggcaccttga cgccaaagcg
     4621 ttcggtctgc cagcacagga agccgccgat aaagggaatt aatattagcc agggtagtaa
     4681 catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca
     4741 cgacagaaaa ttctcttcgg actgggggta gtgtcggatg cgccgcgaag acggcttatc
     4801 cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa cgcagtacca
     4861 tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat
     4921 aaccgttctc gcttaacagc agacctttac ctgcaaagcg ggaaaggaca gccgggatgt
     4981 tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccagg aacggcttga
     5041 cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag gtacccagca
     5101 gacggcccgg cgcactgttg gcgatggagg tcaccagagt acgtttaccc agccatagcc
     5161 aggcagccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc agggtcaaca
     5221 tgctgccgtg cgccacctcc gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca
     5281 gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc
     5341 ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga
     5401 aggtgtaaag cgaggtcata aatgcaccga ccagacctgc caccatcaga ttgatatgtc
     5461 cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg
     5521 gcagtgccga cagtgctgcg ccgcccacca ggaagcagag ataaaccagc ggaatagatt
     5581 tacgcagacc acccatctta aagatgttct gttcgtgatg gcaggccaga atgacggaac
     5641 cggatgccag gaacagcagc gctttaaaga acgcgtgggt catcaagtgg aaaatcgccg
     5701 catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt
     5761 aagcgagaac acgtttgatg tcggtctgta ccagcgccgc aaaaccggcc agcagcagcg
     5821 taaccgcccc aacaatgccc accagatgca gaacttccgg cgtcatcagg aacagaccgt
     5881 gggtacgggc gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg atcagcgcgg
     5941 agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag
     6001 atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccat
     6061 cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt
     6121 tgtaaagaat gaacagcgcg aaagcgagga agacgtcacc cacacgggtc acgacgaacg
     6181 ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaaaccgatc agcagatagg
     6241 agcacaggcc cacgccttcc cagccgaggt acatcaacag caggttatcg gcgagaacca
     6301 gaactaccat gctggcgatg aacaggttgg tgtaagcgaa gaagcgagag tagccctctt
     6361 ctccgcgcat ataccaagag gcatacatgt gaataaggaa acccacacca gtgaccactg
     6421 agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta aagtcgccta
     6481 ccgacatcca cgtccacagc ggctggctgt atgcctgctc accgttagcg aagaagtcaa
     6541 cgccgataaa ggcagttacc agcgccgcca ggcccacaga gccaacgcca acgatcgccg
     6601 agacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca
     6661 aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag
     6721 gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttccgccgc
     6781 cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctggcccc agtagcttcc
     6841 ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa
     6901 cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga ttgccgcgag
     6961 gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg
     7021 tctttacgat tgctcagcac ttcacccgct cgctcttcac gaccaacgtg gaaggccaca
     7081 accagacccg cgagcagcag catagaagcc aattccaccg ccagtacgta aggcccgaac
     7141 agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcgttaaca
     7201 ccgagaatgg cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc
     7261 cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ccgaaccgcc caggttgagc
     7321 atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc
     7381 gcaccggcga agtaagcgcc cagtgagaag aacaccccag agatcgccag cagcgaaata
     7441 atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt
     7501 atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca
     7561 ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga
     7621 ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca
     7681 gcagatcctc tttctcgtaa accagatcct ggcgcttgta ttctcccatt tcgaaatccg
     7741 gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc
     7801 gtgagaagtt gatgcggaaa aattccggat accagcgacc gtctttggtt tctgcttttt
     7861 gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct acgcaacgct
     7921 cttcgccgtc cggatcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga
     7981 ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc
     8041 agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat
     8101 tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt
     8161 cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa
     8221 cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcagcg cgaaccagat
     8281 gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca tcagtgcaga
     8341 gatggtcaca atcccgatgt attcgcccac gaagaacaga ccgaacttca taccggaata
     8401 ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg
     8461 gtgacacacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggatgac
     8521 gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc
     8581 caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc
     8641 acgcatcgca cccagcagcg agtatttgtt gttacttgac cagcccgcaa acagcaccgc
     8701 gtagaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac
     8761 cacccaaccc gggctgactg gcacaatcgc aaaggccagc agcaacgagg taaaggcgat
     8821 catcggcgcc agggtaaaga tgacgcgatc tgagaatttc gggatccagt cttctttaaa
     8881 gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa cacggttagg
     8941 tccgtaacgg ttctggaaca gccccagcag gcgacgttcg ccaaagctca tgaatgcccc
     9001 acaggtgaca accaccagca ggataaccac cgctttgagg atggtcagca ggatctcaat
     9061 cagttccggt gttatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca
     9121 gccagcaccg gagcaatacc ggacataccc atcggcaagc ccacctgccc tgctgtcagc
     9181 ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt
     9241 gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc
     9301 ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg
     9361 tagtacggcg cgatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg
     9421 aagtaatcca gaccgttttc gctggtttca aacagacgca cgcccggatc gccaaaacgc
     9481 agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcctggc
     9541 gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc ttccatcgag
     9601 aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatgttggcg
     9661 cgcatggcgg tacgaccgct gtaacggtgc ggctcacggg ccagtttctg cccacgaatg
     9721 cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca
     9781 acagcgtcaa tcacgtggtc gagctgcgtc cagtccactt cacggctcag cagggtgcta
     9841 tgcagcgagt gcagccagcg ccagctttcc agcatgacag tttggctgtc gtaataagcc
     9901 ggatcgtaaa cctggaagaa acgttgggcg cggccttcgt tgttgatcac cgtaccgtcg
     9961 ctttcagcaa agctggcggc ggaaagtacc agatgagcgt tttccataat cgctgtgcgt
    10021 tgatggtcaa ccaccatcac cagcggcgct ttagccagcg cagcattcac gcgggtagcg
    10081 gaagcgtgac gatgcagatc gttttccagc accaccaccg cgtcggcgcg tccggtttcc
    10141 agttcggtta acgcttcttc aagcgatcca ccgcccataa tgcccagccc catgctgttg
    10201 acggaacggg caatcatggt gataccgacg tcagcgccgc gacctttcag ggctttcgcc
    10261 acgttagccg cagcctgaat cacttcagcg ctaccagcgt tcgtcccgga gataatcaac
    10321 ggtttcttcg caccagccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt
    10381 tcaataccgt caaccgctgg tgcagagtta tccagcgcat gagcgatggc aaaacctaaa
    10441 cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg
    10501 tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt gaggattgcc
    10561 gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt accttttacc
    10621 gcctggcgca ccgccagcgc aacgcgcgcg ccggtctggg taacgtcttc gcccagcacc
    10681 agtaccgcat cgtaagattc gatttcgcgc agtgccggcg tataaatgcc gccttcacgc
    10741 agtactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtatagaag
    10801 ttttcttcgc ccaccagctc acgcagcgca aagttgcttt ccacgctggc acgcggagaa
    10861 ccaataccga tgactttctt cgactgacgc agaatatctg ctgcgccctg cattgcctgt
    10921 tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatct
    10981 ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc
    11041 gtaccgttgt aacggttttc gatacgacgc agttcgccat aacgctcgcc ggggctgatg
    11101 ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg
    11161 ttgtaacgct cggagtgcgt tttgtcggtg aatacgccgg tcgggcaaat ttcgaccagg
    11221 ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg
    11281 tgcgcaccgt aaacgcccag gtccgtaccg tcagcgtaat ctttgtagta acgcacacag
    11341 cggtagcagg cgatgcagcg gttcatttcg tgagagatga acggccctaa atcctgatta
    11401 cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacagtcata
    11461 tcctgaagat ggcagttacc gccctcttca cataccggac agtcgtgcgg gtggttggtc
    11521 atcaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata
    11581 aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc
    11641 gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga
    11701 tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc
    11761 gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca
    11821 gttttctcag tcagagactg agcgttaatc gaaattcggt taccagcgct ctttcagcag
    11881 gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc
    11941 aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg
    12001 ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc
    12061 gcccggctgg ccttcgccac gctccagcgc acgtagaatt ttcacgctcc acggcagacc
    12121 gtcgcggcat ggcgtacacc agccgcagga ctcacgggcg aaaaactctt ccaggttacg
    12181 caccagcgac accatgttga tctcgtggtc aaccgccatc gccagcgccg tacccagacg
    12241 gcttcccgct ttaccgatac tttcgaattc catcggcagg tcaaggtgcg cttcggtcag
    12301 gaagtcagtc cccgcgccgc ctggctgcca ggctttgaat ttcagaccgt cacgcatacc
    12361 accggcgtag tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt cccacagacc
    12421 cggatttttc acccgaccag agaagcccat cagcttggtg ccggcatctt tacttttcga
    12481 gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc
    12541 gacgttgtta acgcaggttg gtttacccca tgcaccggag gttgccggga agggcggttt
    12601 cgagcgtggg ttagcgcgac gcccttccag ggagttgatt aacgctgttt cttcaccgca
    12661 gatgtagcgc cctgccccgg tatggacgaa cagttcgaaa tcaaaacctg ttcccataat
    12721 gtttttgcca agcagacccg cttcggtggc ttcggcaatg gcacggcgta gattcactgc
    12781 cgcttcgata tattcgccgc gcaggaagat atagccacgg taagctttca gcgcaaacgc
    12841 ggagatgagc atgccttcca ccagcaggtg cggcagttgc tccatcagca gacggtcttt
    12901 ataggtgccc ggctccattt catcggcgtt acacagtagg taacggatat tcatggattc
    12961 gtctttcggc atcaggctcc acttcagacc agtcgaaaag cccgcaccgc cgcgcccttt
    13021 cagaccagag tcttttacct gattaacaat ttcgtccgga gacaacccgg tcagcgcctt
    13081 acgcgcgcct tcgtaaccgt ttttgctgcg atattcgtcc agccacactg gctgtttgtc
    13141 atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta
    13201 taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg
    13261 atcatcatgt ttggcccttt atcacagttc ccgaggcagc aagttggcag cagcgtaaag
    13321 cggccatcaa atgtcgtttg ccctggtttg atgttcagtt ttttctccag cgccgcctga
    13381 atgccctggt aaccgttgat atgacagacc acgctgtcgc aataacggat cacatggcgg
    13441 cctactggct gacggaagat ctggctataa aacgttgcca cgccttcgac gtcgctagcc
    13501 ggaataccca gcacatcggc gatcgcgtgg atcgcgccat ccggcaccca gccacgctgc
    13561 ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc
    13621 tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt
    13681 tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat
    13741 aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg
    13801 cataactcgg cgtacggata cgggtgcgat aactcatggt gctaccgtcg ctggtcaggt
    13861 agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg gctggcatca
    13921 ccggacccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt tgcaacgtgc
    13981 gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt
    14041 tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta
    14101 cgcgggtgta gcagtcagaa acaccaccac ccaccgggat ttcaaagtcg aagttttcat
    14161 agccagaata cggacgcgcc ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg
    14221 cgccagtggt gccccactcc agtgcctctt tcgcgccata ggcggcaacg ccctgggaac
    14281 gacctttcag aatggtgttt tgcagcgccg ctttctcgta agacgccaga cgtttcggca
    14341 tccagtcgag gaactctcgc agcaggcgat cccagccgcg cggcaggtcg tgcgcaacgc
    14401 cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc accagatcgt
    14461 aaattttctg acgatcggta aaggcgaaga acactggcgt cattgcgccg acgtcctgaa
    14521 taaaggtcga gatatacagc aggtgactgt tgatgcggaa cagttcggag agcattacgc
    14581 gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacat
    14641 aaggcatttc gttaacgcag ccgccgaggt attcgatacg gtcggtatac ggaatgtagc
    14701 tatgccagga ctggcgctcg cccattttct ccgcaccacg gtggtggtag ccgatgtctg
    14761 gtacgcagtc gacaatctct tcgccatcga gctgcaaaac gatacggaaa gccccgtgcg
    14821 ccgacgggtg gttcgggccg aggttgagga acatgaagtc ctcgttttcg gtgccgcgct
    14881 tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt
    14941 tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt
    15001 gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaatgtaa
    15061 tgccaaatag atcccaggtt tcacgctcat accagttagc gttcgggaat aatttggtaa
    15121 aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac
    15181 gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt
    15241 gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca
    15301 gtttctttaa gaaatcgccc acttccagta attgttcacg cttgatccac acaacgggaa
    15361 ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc
    15421 caatcaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc gcggttaagt
    15481 cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc
    15541 gaaatagatt taggaataag cgaagaaaat tccctttgcc gacaggcgca aattaaatct
    15601 cgtcaggtgt acgcaggttg gtaacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg
    15661 attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt
    15721 ctttaccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg
    15781 gacagcccgg gatatacaca tcaaccggga tgaatttatc aacgccctgc acaacggaat
    15841 aaatatcgta cataccgccg gagttggcac aggcacccat tgagataacc catttgggtt
    15901 ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg
    15961 caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttcagcg ccaaaacgcg
    16021 ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga
    16081 agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca
    16141 taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca
    16201 gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa
    16261 tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc
    16321 ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg
    16381 ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga
    16441 tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg
    16501 ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg
    16561 attcaaacgg aacgtttttt gacctcgcgc gtgcgcgacc gcctaaaaac caaccgccta
    16621 ccagcatcag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat
    16681 gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgcctg
    16741 attctctgct ctttacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa
    16801 ccttacaata actgcagaaa ctattttata cagctaattg atgtggtctt ttactccttt
    16861 ctataacctt ttgtcaactt taacaaaggt ttcttcacac tgatttacac aatgacaaca
    16921 acatttgcat ttggcgccct ttctccccag atcattgacg actccaggaa aattagatgt
    16981 tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt
    17041 tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat
    17101 tctataacaa aaatgcagca acaaattcac gtatttttta acattattgt agcaggagat
    17161 ttttttcagg tgattatttg tgcgtacggg gcgtgaatct ctggtgattg aaaaatgaac
    17221 agttttgtac gttctgcacg atacgaggaa ggctttttac taaaaaaaag ccgctggggt
    17281 ttaaaataaa cccagcggtt cgtttttaca ctattgtctt aggaattatc tatcgtccgt
    17341 cgattactcg atatcccttt caatcaacaa tgagtcatct ccttccggag cagacattgg
    17401 gctgtattgc cacggattat ggtagctctc catcgcctga tagatcacct gggccagctc
    17461 attattactg gacgggtcat agcacagcaa atattcggta tcaggtagcg gcggtaagcc
    17521 atctactcca ctcagcacac gcaggtccgg actcatcatc tcgaccggcc ttgccgtcac
    17581 gccaagaccg gctttcactg ccgcacgaac ggccggaagc gtcgaggcga cataagccag
    17641 tcgccaggga atatctgctt tattaagcgt cgccagcacc atatcgcgaa acggacttgg
    17701 atcatccagc aacacaagag ggatcggctc acctttttgc aatacgtatt ccgctgcgca
    17761 gtaccagtgt gttggcgagg tacgcaggtt caatgcctta aaggtagaag gacgatgggt
    17821 ggttaccatc aaatccactt cctgggattc cagcatttct gccatgtagg cattacgttt
    17881 aacgcggaca tccagcgcta gtttcggata aaccgaactc acgcgattta acaggaaagg
    17941 taagatcgta tcggcagatt catctgaagc accgatagtt aacacgccct gaagattact
    18001 gaacattaat gatgagcagg cctcatcatt aaaacgcagg attttcctgg cgtaaccaag
    18061 aagttgaatg ccatgttcag ttaacagttt gttgcgaccg tgacgagcga acagttcttt
    18121 cccaacgagt tgttccagac gctgcatttg ctgacttacg gcggactgag tacgacacac
    18181 agcggcagct gcggcagcaa aagtgttcag atcggcaaca gcaacaaatg ttctcagcag
    18241 atcgaggtcg aggttaatta tcggacgatt tgcacttatc atatattatc acttactggc
    18301 ggctcatact gagctggtta atgctgtgca cacacaaaca agcaattcca tttgtaatgt
    18361 gcctccctgg cagtttcatc cggaacccgg acgaaagtaa aaatgcatat gagttgcact
    18421 aaaaaagtga ctcacattgt tccgttataa tgcctgaagt agatcacaga atatatcttc
    18481 agggatcgca tatctattaa gttactcact cttttctatt tatgacatgc gcgtgtttgt
    18541 ataaatgtaa atgtgagtcc ttgttccact ctcgtacagc atcgctggtc atacgcgaac
    18601 acgtacaaac agcaatggtg aggcggcatc aagagcaggg atccgtcatt tatgcaagca
    18661 ttttacccca aaaacctttt atttataagg gtcattgcga attatctgat gcaaagttat
    18721 gttatgttat gttaggcaaa gtgatcttct attattaata agcacaacaa aa
//
LOCUS       DAZKHK010000101        18652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKHK010000101 DAZKHK010000000
VERSION     DAZKHK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.101"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..843
                     /gene="hyxR"
                     /locus_tag="ACHOS5_003628"
     CDS             49..843
                     /gene="hyxR"
                     /locus_tag="ACHOS5_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFP9778386.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLARLRSDAGQWQVCLTGDSDWFDNAPAASLYNGFP
                     RIGTMLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            997..1758
                     /gene="hyxA"
                     /locus_tag="ACHOS5_003629"
     CDS             997..1758
                     /gene="hyxA"
                     /locus_tag="ACHOS5_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFP9778387.1"
                     /translation="MRFVLFCPSGIVPAQFAALSTGSVGNVTCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VERVISIANASGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHNWEYSAQFLKTGIRQIWLTEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LETTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1800..4097
                     /gene="hyxB"
                     /locus_tag="ACHOS5_003630"
     CDS             1800..4097
                     /gene="hyxB"
                     /locus_tag="ACHOS5_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFP9778388.1"
                     /translation="MKNSKAFYRSALATAIVMALSAPAFAADNAVSTDSVTLNKDKTT
                     LDQDVVINNTADKQITAVTINAANEDLNVTFAGHDITAESTADKKFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVISSTGDVFVNGGNFVAKNEKGSATGISLEATTGNN
                     LTLNGTTINAQGNKSYSNGSTAIFAQKGNLLQGFDGDATDNITLADSNIINGGIETIV
                     TAGNKTGIHTVNLNIKDGSVIGAANNKQTIYASASAQGAGSATQNLNLSVADSTIYSD
                     VLALSESENSAGTTTNVNMNVARSYWEGNAYTLNSGDKAGSDLDINLSDSSVWKGKVS
                     GAGDASVSLQNGSVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGNLSVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYINTADVRLMNGGGTIKADFNTNGFGAM
                     VKGGYTWKDGNGLFIQPYAKLSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDF
                     AVGNATVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
     gene            4282..4950
                     /gene="ykgH"
                     /locus_tag="ACHOS5_003631"
     CDS             4282..4950
                     /gene="ykgH"
                     /locus_tag="ACHOS5_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP9778389.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVTYSLKINQTTPGILVSCDSNNNFSCQTTMTEDVIQRITTFFQTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(5193..5888)
                     /locus_tag="ACHOS5_003632"
     CDS             complement(5193..5888)
                     /locus_tag="ACHOS5_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9778390.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5881..7308)
                     /locus_tag="ACHOS5_003633"
     CDS             complement(5881..7308)
                     /locus_tag="ACHOS5_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP9778391.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCLAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7319..8038)
                     /locus_tag="ACHOS5_003634"
     CDS             complement(7319..8038)
                     /locus_tag="ACHOS5_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9778392.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYTKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(8552..9406)
                     /gene="rclR"
                     /locus_tag="ACHOS5_003635"
     CDS             complement(8552..9406)
                     /gene="rclR"
                     /locus_tag="ACHOS5_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP9778393.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            9633..10958
                     /gene="rclA"
                     /locus_tag="ACHOS5_003636"
     CDS             9633..10958
                     /gene="rclA"
                     /locus_tag="ACHOS5_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP9778394.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTANNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            11067..11303
                     /gene="rclB"
                     /locus_tag="ACHOS5_003637"
     CDS             11067..11303
                     /gene="rclB"
                     /locus_tag="ACHOS5_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP9778395.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            11315..11908
                     /gene="rclC"
                     /locus_tag="ACHOS5_003638"
     CDS             11315..11908
                     /gene="rclC"
                     /locus_tag="ACHOS5_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP9778396.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(12068..12937)
                     /locus_tag="ACHOS5_003639"
     CDS             complement(12068..12937)
                     /locus_tag="ACHOS5_003639"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9778397.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            13185..14042
                     /locus_tag="ACHOS5_003640"
     CDS             13185..14042
                     /locus_tag="ACHOS5_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9778398.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMSKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(14167..18417)
                     /gene="fdeC"
                     /locus_tag="ACHOS5_003641"
     CDS             complement(14167..18417)
                     /gene="fdeC"
                     /locus_tag="ACHOS5_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP9778399.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKTSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTSLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEASVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADNTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
BASE COUNT         4904 a         4509 c         4224 g         5015 t
ORIGIN      
        1 tttgaatcaa aatgtcaggt tttgtagcaa tggcgtggac gagtctgaat gaagaagcct
       61 ctggttatta tcagtgcctg tcagttcacc aggctggcac tggagtctct catcccaacg
      121 gacaggtata tcgtgcgggt gtacagcaac gtcactacag aggttgaatt cgttttaaag
      181 acctcctgtg gctacctgtt agctgatatc ccttcgcttc cttcggggga gttggttctt
      241 ctggcttgtc tcgcccgttt gcggagtgat gccgggcaat ggcaggtctg cctgaccggt
      301 gactccgact ggttcgataa tgctcctgct gcaagtctgt ataacggctt cccacgcatc
      361 ggcaccatgc tgacagcgag tcgttatcag aatcaaatta tccgctggtt actacgtcct
      421 ttgcccggcg atatgagcga tttctggctc ctgactgcac gggagttgag cgtgcttaag
      481 attctgatgc agggcatgag tttcagtgag attgcccgtt atgaacaacg cagttcaaag
      541 accctgcatg cgattgccac acgggcttta atgaaacttg gactgggaac actgagcgat
      601 ttcaggctgc tctatacggg atgcggtgac agcagagtga aacggaatat acggctacat
      661 gcaagatatg tcggacaaca ttccgctcat cgactcactc agtatgtgca gaacatggta
      721 tccggaattc tttatccaga gaaagttgcc gttggtgaat caataaattc tgacgccata
      781 tccggaaaag tactttccgg acaaagaaaa aatatgtcta aaaaaagacc aggattgtta
      841 tgacacagca gcgttgttac taccccccac tgcggcgcat acggactcgc tacagtgtag
      901 gagccaaaat atttctactg ctctgggctg caggattggg aggcacgctg tacagcctgt
      961 acagcgtgct ggcgtactga catctgtgag ggaaggatgc gtttcgttct tttctgtccc
     1021 tcaggaattg taccagcgca gttcgcagct ctatcaaccg gttctgtcgg caatgttacc
     1081 tgcatcagaa cagaagagga gttacgcaat aaattgcggc ataggccaca gagtgtcgtg
     1141 atatcggcag ggcgacctgc cgaatgcgct gaaatgtggt tccgatttta tcgggatcac
     1201 tcattcgtgg tggtgttgtg cgtcgcgccc ttttttctgc caccggatgt gagtatttct
     1261 ggcgttctca aaaacctgag gttactgaaa ccaggaatgt cagtagagcg tgtcatcagt
     1321 attgcaaatg cctctggtgg gttttcaggg ctgaaacatg cagaaatttt gccagttatg
     1381 gacagttact ctgtctttat gaaagaggtg aataaccgca caaagacaat cgtcatgtca
     1441 gaacgcttcc cggaaaagca aaagaaggtg ttgtcgttac ttcttgccgg acacaactgg
     1501 gaatacagtg cgcaatttct caaaacgggg ataagacaga tatggcttac cgagcagtcg
     1561 ctaaaaaagc gttggggaat accggacagc atgtcattgc gggaagccct gctgctgagc
     1621 agcaataaat ttcacggtga tggaaacgca ttggaaacaa cgaatgccat gacattgcgt
     1681 gaaaacggaa ataccaacag gtattccgtg gtggtaaatg cagggacgca ggctttatct
     1741 ctgcataaat ataaataagt ggaaaattga aattagttac cagaatggag aatttcttca
     1801 tgaaaaacag taaagcattt taccgcagcg cattagcaac agctattgtt atggctcttt
     1861 ctgcaccagc attcgctgct gataacgcgg tatcaactga ttcggttacg ctgaataaag
     1921 ataaaacgac tctggatcaa gatgttgtta ttaacaatac agcagacaaa cagattacag
     1981 ccgtaacaat taatgcggca aatgaagacc tgaatgttac ttttgctggt catgatatta
     2041 ctgctgaatc aacggcagac aaaaaattcg ttgaaggtgt aaaagttagt ggtaacaaaa
     2101 atgttgtgat taatgctaca gactccacca tcacagctca aggtgaaggc acctatgtcc
     2161 ggactgcaat ggtcatttct tcaactggcg atgtttttgt taatggcggt aatttcgttg
     2221 caaaaaatga aaaaggtagt gcgacaggga tatctctgga agcgaccacg ggaaataatt
     2281 taacgctcaa tggtacaacc ataaatgctc aaggtaataa gagttacagc aacggctcta
     2341 cggcaatttt tgctcaaaag ggtaatttgt tgcagggttt tgacggtgat gcaaccgaca
     2401 acatcaccct tgctgactca aatattatta atggcgggat tgaaacaata gttactgccg
     2461 ggaataagac gggaattcat acagtcaacc tgaatattaa ggatggctca gtaattgggg
     2521 cggctaataa taaacaaaca atttatgcat ctgcttcggc acaaggcgca ggttcagcaa
     2581 cgcaaaattt aaatttgtct gttgctgatt caaccatcta ctctgatgtc ctggcccttt
     2641 ctgaaagcga gaattcagcc ggtaccacaa caaatgtaaa tatgaacgtt gcccgctctt
     2701 actgggaagg taatgcttat acgctcaata gcggcgataa agcgggtagt gatctggata
     2761 taaatctttc cgatagttca gtctggaaag gcaaagtttc aggggcagga gatgccagtg
     2821 tatctctgca aaacgggtct gtctggaatg ttacgggttc ctcaactgtt gatgctctgg
     2881 cagtaaaaga cagtacggtt aatatcacga aggctacagt caatactggc acgtttgctt
     2941 ctcaaaacgg cactctgatt gttgatgcct cttctgaaaa caccctggat atcagcggta
     3001 aagcgagcgg taacttgagt gtttacagtg cgggttcatt ggatcttatc aatgaacaaa
     3061 cggcatttat ttctaccggc aaagacagca ctctaaaagc cacaggcaca acggaaggtg
     3121 gtctgtatca atatgacctg acacagggag ctgatggtaa cttttatttc gtaaaaaaca
     3181 cgcataaagc atccaacgcc agctccgtga ttcaggcaat ggcagctgct ccggctaacg
     3241 tcgctaatct gcaggctgac acgctctccg cccgtcagga tgctgtccgt ctgagcgaaa
     3301 atgacaaggg tggcgtatgg attcagtact ttggcggtaa acagaaacat accaccgcgg
     3361 gaaatgcatc ctatgacctg gatgtaaatg gtgtaatgct gggtggtgat acccgcttca
     3421 tgactgaaga tggtagctgg ctggccggtg tggcgatgtc ttctgcgaaa ggtgacatga
     3481 ctaccatgca gagcaaaggt gacactgaag gttacagctt ccacgcttac ctgagccgcc
     3541 agtataacaa cggtatcttc attgatactg ctgcacagtt tggtcactac atcaacacgg
     3601 cagatgttcg cctgatgaat ggtggcggta ccatcaaagc tgactttaac accaatggtt
     3661 ttggtgcgat ggttaaaggc ggttacacat ggaaagacgg taatggcctg tttattcagc
     3721 catatgccaa actgtctgct ctgactctgg aaggtgtgga ttatcaactc aacggcgtgg
     3781 acgttcattc tgacagctat aactctgtgc tgggtgaggc cggtacgcgc gtgggttatg
     3841 acttcgctgt gggcaacgcg accgttaaac cttatctgaa tctggccgca ctgaacgaat
     3901 tctctgatgg caacaaagtc cgtctgggtg atgagtctgt caatgccagc attgacggtg
     3961 cagcattccg cgtgggtgca ggtgtacagg ctgatatcac caaaaacatg ggagcatatg
     4021 ctagccttga ctacaccaaa ggtgacgaca ttgagaaccc gctacagggt gtagttggta
     4081 tcaatgtgac ctggtaagaa taaagcagtc ggttcccctg tcgactgtta tacccccctg
     4141 caggcagcag tatgctgcct gcaggctata aatatccagt attcatgatg ctggcttttt
     4201 gctgatattt accctgaaca ttcctgatac agactattaa tacaatctta tctgcgacag
     4261 atttcatttt ctggatatat tatgaatgaa caaatcaaac aggatatcga tctgattgag
     4321 attttatttt atctgaagaa aaagattcgt gttatccttt ttattatagc tatctgtatg
     4381 gcaatggtgc tgttgtttct gtatatcaat aaagataata taaaagtgac ttacagtcta
     4441 aaaataaacc agacaacacc tggcatactt gttagctgtg atagcaataa taacttttcc
     4501 tgtcagacca caatgactga agatgttatt cagcgaatta ctacattttt tcagaccagc
     4561 ccagatgtca agaacagaga aataaagctg gaatggtcag gagataagag agatttacca
     4621 actgctgaag cggaaatatc tcgcgtgcag gcgtctatca ttaaatggta tacgtcagaa
     4681 taccataatg gcaggcaagt tctcgatgag atacaaacgc cttcagcaat taacagtgag
     4741 ctttatacaa aaatgatata cctgaccagg aactggtcac tgtatccgaa cggtgatggc
     4801 tgtgtcacta taagctcacc agaaataaaa aataaatacc ctgctgccat ttgcctggct
     4861 ctgggatttt ttctaagtat tgtgatttct gtaatgtttt gccttgtcaa aaaaatggta
     4921 gatgaatacc aacaaaactc tgggcagtaa taaacagcat tctgacagag agtgcctgta
     4981 taaaactata gtgacagtga ccgggaaggg agcccgtctt caggaatgga ttcctgtctt
     5041 tttaactgta aatatcctgg cattattaat gtcaggatat ttacagaaaa gcagcataac
     5101 catgacggag cggggttaag tcaggttgtc cttaacgtta ttcacaacct cgtatgccga
     5161 cttcaggttt atgtccagac tacatatctc tctcagcaat cctcaataat cagatacacc
     5221 gctttcaccg gaccatgaac cccgacgact ttgataagct caatgtccgc cgttgaactg
     5281 ggaccactga tgatgttaat acatgacggc attcgttcgc cggcctgcgc tttctgatgc
     5341 aatttttctg caagttgcgc tacacgcggc aggattgtgc ttttacgcag gataaaaaga
     5401 gaagattccg ggagcaggct caatgaacgc ccgcgcccgg cggcggaaaa aagaaccacg
     5461 cctcccgatt cggttaaacc atattcagca tatacgacgc ccactttagc ctgctctgct
     5521 tgtgatatgt tctcaatacc tttcgccgga tcccaaacaa cggcattgca ttcctgctgc
     5581 aaacgttcgc taatccccaa ttcctccagc ctcgtgtcac cactaatcac gaccgactga
     5641 tctcccagct ctttacacag acgtattgca gcttctgccg ccttcgcctc gctggtcagt
     5701 tcacagcgcg tcaacataac atcgctggca aactggataa acgcgtcaca gcgctgctgt
     5761 tgggtaagtt gggtaagccg ctcgttagca tagttgttaa gcggcgcatc ttctgcttgc
     5821 ggttcaagtc gcagcgggcg acccagtgcc tgagcaacgt tattcaaaaa ttcgctccga
     5881 ttatccattc tttttctcct gcgcctgatg tttcttaaac caactacgga aactctctcc
     5941 gtcagcttca ggaagatcgc gtgcttccat ccagtcgcta atcgcgccaa atttgagtgg
     6001 tgttttgccg ccattgataa accagcttgc cgcatgagca ccagccatca tcccaacttt
     6061 ccacaatcct ggatgactat tggcataagc gaacattttt atcgcccgtt gctctgcttt
     6121 tgcggtgatc cctttttcag ccatcacccg acgatgacgc aaaatcagtt ttgacagcgg
     6181 aatacgcacc ggacacacgc tgtcacaagc ggtgcataaa gagcaggcgt agggtaaatc
     6241 tttaaaatct ttatagccgc caagtaacgg ggaaatcacc gcaccaattg gccctggata
     6301 aatagagcca tatccatgac cgccaatatg gcgatatgcc agacaagtat tcatacaagc
     6361 cccgcaacga atacagcgca gcacatcccg aaattcagag gccagcacct cagaacgccc
     6421 gttatcgaca ataaccagat gaaactcttc aggaccatca acgtgccccg cttcgcgcgg
     6481 ccctgtcagc caggtgttgt atcccgtcaa acgtgcacca acggcactgc gcgccagcat
     6541 ggtgatcaat acatctacct ctgcaaacgt gggagcaata cgctccattc ccatcactgc
     6601 aatatgcgtt ttaggcagcg tggtacacat tcgcgcatta ccttcattgg tcaccaggca
     6661 taccgaaccg gtctctgcca ccgcgaaatt acagccggta atacctattt cagcactgag
     6721 gaaatcttcg cggatttttt gccgaatgaa taaggtcatc gcttcaggcg tttccggccc
     6781 ctcatagccc agacgttcgt gtagcactcg acggatctga tggcgatctt tatgaattgc
     6841 cgggaccaca acatgagagg gcggatcttg atccaactgg agaatgtatt cacccagatc
     6901 ggtttcaatc acctgaatcc cggcatcctg caacacatga ttgacaccaa tctcttcggt
     6961 caccatcgat ttagatttca ctaccttccg ggcatttttg cgttgggcaa cctgtaaaat
     7021 gtagcgggtg gcgtcttctt tggtttttgc aaaatagacg tgaccgccgt tttgtgtcac
     7081 tttttctgag agctggtaca gataagcgtc gagattactc agaacatgat cacgtatctg
     7141 ggcggcccga tcgcgccact cctcccagtg ccccaattca tcgaccattt tttgccgatt
     7201 tgccccaata cgctgctgcg cgtttgccac cgctttacgc atgatcggat cttcaatttg
     7261 ctgacggatg cgtgtcttaa aatctgtatt actggttttg atcgacatct ttatatcctc
     7321 agcggctcat cagcacttca gcaatatgca tcactttgac tttctgccct tcccgttgta
     7381 atcgtccact gatgttcagc aggcaactca cgtcagcacc aattaaatac tcagggcgga
     7441 cttccatcag gtgcgcaacc ttttctttca ccatctcgcc ggatatttcg gccattttga
     7501 ccgagaacgt gccgccaaat ccgcagcagg tatcctgttc agcaaaggtc aacagctcca
     7561 gtccacgcac atttttcagc agcgtaagag gttcgtcctt cacccccagc ttacgggcca
     7621 ggctacaaga tgggtgatac accgctctcc cttgcaaact ggcacctaca tcgactaccc
     7681 ctaatgtatt aacaataaaa gaggtgagat cctgcatacg cgcggcaacc ttttcggcac
     7741 gtgatgccca ttcaggttca tccgccagat acgtcgggta actttttacg gcataggtgc
     7801 aagagccagc cggtgaaata atgggatcgt cgttatcctc cagtgcggcg atcagatttt
     7861 tcatccctgg aatcgcttct ttggtataac cactattgat cgcaggctga ccgcagcatc
     7921 cctgtttctc cgggaaattt acgcgacagc cgagtttttc cagtagcagc acggagtctc
     7981 gtgccattct tgatttcagg gcgtcaccaa tacaggtgac aaagaaattg acattcacaa
     8041 ctaatactcc attacttcat gcccatttat gcccgatggt tccccccttc cgggggatca
     8101 atcatcaata aatatggcgt tcttttttaa ttggctagca acataccacc cctcatttgt
     8161 atgacaaatg ataatctttc atctaaactt caaatgcaat aactaatttt atttctttcg
     8221 gcatcgctca cattttatgg ggaaatcgac gatcattttt ggcataagaa atctatttaa
     8281 aaattgctac atcctttata cctattcagg gggtgttgag atatacactg atggtgtaca
     8341 aacaaaatca ccgacaataa tcacgtatca agtaatagtc gggattttat ttaattgaag
     8401 aaatttagtt tgcattttta tttaaaaact gagctagagt ccatcaaaaa aatatataaa
     8461 acccaaaacc accgttggaa atagaaatag ttgaaaggct cacaactccc ggaatgcgga
     8521 ttatctttgc cctgatttct ggcggtttta ttcagggtgc aagctgtctg accctttccc
     8581 gatattctcc cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa gatgattcgc
     8641 tggcatagcc tactgactca gcgatcacca caacagggag catttcccgg gaaaacatct
     8701 gggccgctat ttgtagacgc aactttgtta atacagccag cggcgtggtt ccggaaacat
     8761 cacggaaaag ctgggcaaaa cttgcccggg acatgtgggc gatgctggcc agcgattcga
     8821 cggtccaggc gtgtccaggc atttccatca tttgctgtat taccgcacca agacgtggat
     8881 gcagaagcaa actgagaatg tttttctctg tattaacctg tgcaatccat tcacgcaccg
     8941 cgagggtaaa gaatgtagcg cagatctggc tacacagagc atccactccc ggcattgccg
     9001 atctggattc ctgttgtaaa aacgggatcg cctccctcag ccagttatat tccacgctgt
     9061 ggttaaccgg tgctacaaac agcgtttccg gcaaagacgt taaaaaataa cgcgccgaat
     9121 gttgcagccg aagagttcca cagacaatac aggtcgattc attatcgaca tgactcaggc
     9181 gatgagctga attttgtggt agcaggacca catttcccgg gcgtaatgta aaaatctctc
     9241 ccgtcggcat ttccagcttc gccgctcctt gcgttaacgc atgccaacga ataaccgaca
     9301 attccccggc accatgtgga agttgccagt cacttcctaa cacgcaattc ttatcgatcg
     9361 ttccttgtgg agcgttaagc atcaacaaac ggctaagagc atccatatca gactcctgag
     9421 tggttttttg attacaaaca ccaacaatct tagtcaataa ttaatctaat accatcaaat
     9481 agttaatcat gatttttgtt ttgtgattct gattaagaaa aagaaaatac cagacgataa
     9541 gacaaaatat caagcgttgt gaagaaatgt tatttgctct tttgcgtcta tagtcatgat
     9601 gtcaaatgaa cgcgtttcga caggaaatca tcatgaataa atatcaggca gttattattg
     9661 gttttggcaa ggctggaaaa acattagcag tcacgctggc aaaagcaggt tggcgtgtgg
     9721 ctctcatcga acaatcaaat gcaatgtatg gcgggacctg tattaatatc ggctgtatcc
     9781 caaccaaaac attggttcat gacgcacagc agcacacaga ttttgtccgt gccatacagc
     9841 gtaaaaatga agtggttaat tttttacgta ataagaattt tcataatctt gcggatatgc
     9901 ccaatatcga cgtgatcgac ggccaggcgg agtttatcaa taatcatagc ctgcgtgttc
     9961 atcggcctgg gggaaatctg gagattcatg gcgagaaaat ttttattaat accggtgcac
    10021 aagccgtggt tccgccaatt cctggaatta ccaccacgcc tggtgtatat gacagcaccg
    10081 gattacttaa tctaaaagaa ttgcctgggc atttaggtat tttgggcggc ggatatattg
    10141 gcgttgagtt cgcctctatg ttcgctaatt ttggcagcaa agtaaccatt ttagaggcag
    10201 cttcgctgtt tttgcctcgg gaagatcggg atattgctga taatatcgcg acgattttac
    10261 gcgatcaggg tgtcgatata atcctcaatg cccatgtgga gcgaatcagt caccatgaaa
    10321 atcaagtgca agtgcatagc gagcacgccc aactggcggt ggatgcactg ttaatagctt
    10381 ccggtcgtca accggctacc gcttcgttac atccagaaaa tgcaggtatc gcagtaaacg
    10441 aacgtggggc aattgtcgtt gacaagcaat tacataccac cgcaaacaat atttgggcga
    10501 tgggagatgt taccggcggg ctgcaattta cttacatttc actggatgat taccgcattg
    10561 tgcgtgatga gttgctggga gaaggcagac gtagtactga tgatcggaaa aatgtgcctt
    10621 attccgtatt catgacaccg ccactgtcca gggttggtat gacagaagaa caggccagag
    10681 agagtggtgc tgatattcag gtggtgacat tgcctgtagc tgcaattccg cgtgccagag
    10741 tgatgaatga tactcgtggg gtattaaaag cgattgttga taataaaacc caacgtatat
    10801 taggggcatc actgctatgt gttgactccc acgagatgat caatatagtg aaaatggtga
    10861 tggatgccgg gctgccttat agcatattac gcgatcagat atttactcat ccgtcgatga
    10921 gcgaatcact caatgatcta ttttcattag tcaaataaac tcaaaatcag acgccagaac
    10981 aaatattctg gcgtctcaga gaaaagaatc ttattaattc cctgttactt tataacttct
    11041 taatcacttc attgatggta ttttatatgt ttaaaaaatc tgttttattt gcaacactat
    11101 tatctggcgt tatggcattt tccaccaatg cagatgataa aataattctg aaacatatca
    11161 gcgtctcatc agtatcagca tcaccgacag ttcttgagaa tgccattgct gatatggcca
    11221 gaaaatataa tgcttcatcc tggaaagtta catcgatgcg aattgataat aattcaaccg
    11281 caacagcagt attgtataaa taaggatgtt tacaatggaa agatacctgc acctgttaag
    11341 tcggggagat aaaattggcc tgacattgat tcgtctgagt attgcgattg tttttatgtg
    11401 gattgggtta ttaaagtttg tcccttacga ggcagacagc attacaccat tcgtcgcaaa
    11461 cagtccacta atgtcgttct tttatgaaca cccggaagac tataaacagc atctgactca
    11521 cgaaggcgaa tacaaaccag aagcaagagc atggcaaacg gctaataata cctatggttt
    11581 ttccaacggt cttggcgtcg tggaggtgat tattgctctg ctggttttgg ctaatcctgt
    11641 caatcgctgg ttaggtttat tgggtgggct gatggcattt accacgccgc tggtaacact
    11701 ctcattttta atcaccaccc cggaggcatg ggtacccgca ttgggtgacg ctcatcatgg
    11761 tttcccttat ttatccggtg ctggtcgtct ggtattgaaa gatactctga tgctggcagg
    11821 tgctgtcatg ataatggcag attcggcgcg ggatattctt aaacaacgca gtaatgaatc
    11881 cagttcaacg ttaaaaactg aatattgata atccgaacac tgtctgttgt tcactttttc
    11941 aggcgtcagg ctccctttct gatgatgaag cctgacgcca tattaccgga gatatgacag
    12001 agtgaaggtc aatatcactc ccctattaaa agattcttgt cgtctcaggt aaaataagtg
    12061 attaatatca atcatgtatt tttacagcaa gacaaccacg aacaaactca ggattaaaat
    12121 gttttaccga ttccccaaca taacccaaat caagcgaact aatttgcgcc atttctttct
    12181 ctgtcagtga gaaatcccaa atagcaaaat tttcttcgat acgctgttga tgtgtcgatt
    12241 tcggaataac gaccactccc cgttgaatat tccaacgcag aatgacctga gaaatcgatt
    12301 tttgatgggc atctgcaata ctctgaagca gattattttc aaagggttta tgtcgtccac
    12361 cacctaacgg agcccatgct tcaggctgca cgttataata cttcatggtt gccagcgcct
    12421 ctggttgggc aaaataggga tgcaactcga cctggttgac catcggttta actcttaccg
    12481 tttcacaaaa gttcgccaga acatgagggt agaaattgga aaccccaatc gcttttaatt
    12541 tgccctcttc ataggcatct tcgagtgcac gccacgcact gaaataatcg cgcatggcct
    12601 ggtgcaataa atagagatcg aagtattcta gccccgattt ttttaatgat gcttcaatac
    12661 ctgctgctgc aatatcctga ttcaacatat cctgcaccca caatttagac gttataaata
    12721 actcttctcg ggtacataaa ccttcagata tggcctcacg aacggcttca ccgacggcat
    12781 cttcattacc gtataccgct gctgtgtcga tgagtcgata acccgtgcgg atagcattca
    12841 acactgactg tttgcacacg tctttatcgg taacctgaaa aacaccaaat cccatcatcg
    12901 gcattttcag attgttactt aatacggaaa attccactgt cagtccctcc attaggtatg
    12961 agcaatgagt ctacgttaaa acgtaacctc aaattagtat gtggattttg atatcactta
    13021 tgcaaaaaat tcattaataa tggagggcag aaatctctct atttttgggg caatgaagca
    13081 gacgctatca gtgctttttc cttgcgcctt gctgtttttg atacacttag ggcccaggac
    13141 ataacgaaaa tgtgcgatat gacaaagaat taacggagaa tgtgatgatc aggcagaaga
    13201 ttctacagca gctcctggag tggattgagt gcaatcttga gcaccctatt tcaatcgaag
    13261 atatcgcaca gaaatctggc tacagcagac gcaacatcca gcttctgttc cgaaatttca
    13321 tgcatgtgcc tttgggagaa tacattcgca aacgcaggct ttgtcgtgcc gccattcttg
    13381 tccggctctc cgcgaaatct atgcttgata ttgcactctc tttgcatttt gattcacagc
    13441 agtcattcag ccgcgaattt aaaaagttat tcggctgctc tccccgtgaa taccgccacc
    13501 gtgattattg ggatctcgca aatatcttcc cttctttttt aatacgtcaa cagcaaaaga
    13561 cggagtgtag attagtcaac tttcctgaga cacctatttt tggcaattca tttaaatatg
    13621 atattgaagt gtcgaataaa ttaccggatg aagaagtcaa actgcgacgt catcatttag
    13681 tcagatgtat gaagaatttt aagacggata tctatttcgt ctccacgttc gaaccgtcaa
    13741 caaaatcggt cgatttgctc acagttgaaa cttttgccgg tacggtatgc aaacaaaccg
    13801 attcagagat gtcgaaagag tggacaatta accgaggact gtatgcctct ttccgctatg
    13861 aaggtgagtg ggagcactac cctgaatggg cgcgaaacct ctatctgatg gaattacctg
    13921 ccagggggtt agccagagtg aacggcagtg atattgagcg attttattac aatgaaaact
    13981 tcgtcgaaat tgatagcaat aacattgttt gcgaaatttt tatccccgtt cgcccggttt
    14041 agcctgtaac tccatcacat aagatttacc ctaccctgcc accgtctaat cggtaccagg
    14101 gtaggtattc agtaaataat ggcgctgcgc atgtcaacgc cattattatt tattacatcg
    14161 tcgtttttat ttctcctcag cgccttcagt atctgcagga acggcgttaa ttgtcacagt
    14221 cactggtttc atttcatacc ctccatatgt caaagagttg aatgtgaatg tgtatgtacc
    14281 tggcttgttg gtactaaatg tacgcgtgat tttcccgctc gcgaaatggt catttttcga
    14341 cggcaggaac tgataatccg cctctgtcac cccttccgga gcctcaatgt cgacccacat
    14401 ggttcctcct acggggttga cacccgacat cagagtaacc gtcagtgttg cagtagtctt
    14461 cccgtccgcc accaaagaat ctacacgctc tggagatgca gcgagcaccg cgtttttatc
    14521 atccgcgacg aatttagatt cggcagtttt cgtcgattcc tgaccgtcgg cctgttccac
    14581 tttcgccgtg agtgtatatg tcgctgcgac agtggtcgtg gcggtgaaaa tagcctgccc
    14641 ttgctcattc gttgtcgccg tcccattggg agtcagaact aaattttccg ggctggcagt
    14701 cagcgtcact tcgctatctt tcagcaggtt gttattggca tcagtcaccg taactttata
    14761 cgtctgtttg gttttgccgt ccgccactgc acggttgcca atcacctcaa tactggagat
    14821 ggtcgccgta gtgttatcgg ccacaaacgt tacagactgc gcattcgaag gctggtcatt
    14881 tcctgctgac gccgtgatct cataggttcc tgcagtcacg gaaaccactt tcagttccgc
    14941 tttaccctcc ttgtcggcgt tcaccatgat attaccgtct gcaagcggtt tgacgccccg
    15001 aggcagatta aagacgacca cagcgccagg aagaaggttg ccgtattgat ccttaaccgt
    15061 tgccgtcagc gtaaaggcat ctttgccgtt tgccactttt tgagcagcgg cgtctacctc
    15121 caggctcgcc tgaccggtac tgaaatccgc cttgaatttc accttgaccg tcttctgaga
    15181 gtttttcacc gaggcctcga tctcaagttc ccctgcaacc gttgacataa gctcaatgtc
    15241 cactttccct gccgcattgg ttgttactgt attccccgtc ttgctggtca caccctgcgg
    15301 taaagtcagc gtaacttctt gcccctgcaa cgggttacca tagctgtcca cgacggtcag
    15361 ggttatctgg ttagcagact gtccattagc cagttggtta ttaaccttca ctgtcatatc
    15421 acgaatctca gccttagatg cgtcacctgc aacattcagc accagtggct gagcaacggc
    15481 attttggccg ttcactcgcg gcatcacaga caactgaccc gcggcagatc ccagcgttaa
    15541 ggtcgacacg tagaccccat tacctttctc tgtccagttt cctgctgaag gacgctcact
    15601 ccccgtgctg gcggcaccac taaacactgg tgcatctggc ttcagcccgg tgaccggatt
    15661 cccgtacgca tccttcacgg tgaaggtgag ttccgtcgtc gttttaaccg ttggagtttt
    15721 attgtccgca acaagcgtag agttcgctga tgacatctct ccggcaataa cagtcagctg
    15781 cgcggcttcg gtggctgcag gctggccgtt gaagagcggc atgacacgaa gctcgcccgt
    15841 ctttccgcct gtagttaacg tagcaacata ggaaccgtca cctttttcgg tccaactgga
    15901 aacggtcgcc ccttcagagg cggtccccgt caaacttgcc gacaacgaaa gaccactgat
    15961 agcgttgcca tgcgcatctt tcgccaccag cgttacggtt gtgctttcac cggcttttac
    16021 gtgatcttct gcgacagaga ctttcgactg gtttgaagat aatgcatcag ccaccacggt
    16081 tacttttgcc gcatttgctg ccgcgtcctg cccattgagc ttcggcataa cgtctaattc
    16141 acccgccgta gtgccgagag aaatctgcgc agtccaggtc ccgtcgccat tatccgtcca
    16201 gcctgatgcc gtagaaccag cagcagctgc gcccgataat gacggtgcat ccggattgag
    16261 gctggtcaca gggttgtcat tagcatcttt tgccgtccag attgccgtaa ctgtaccgcc
    16321 aaccaccggt ttatcaggat tcagtgtgat ggacgaatgt gctgcatcaa gcggcccggc
    16381 aacaaacttc agcgtttgtt gtaatgtgcc cagctgatac tgctcgctga aggcacgcac
    16441 aacaacgttt ccggcacggg tcgaagaaac cgtggcgctg taaacccctg gttttatttc
    16501 cgaaatggca ccaacggtta caccattagt gtcttgcgga acaagtcgca ggcggctggc
    16561 ttctcccgtc accggattac cctcggagtc caccgctgtc agtgtcagcg tgtaggcttg
    16621 ctgaccatca gcaaccacat cacctgacgg ctcgttagcg ctcagggtgg agtttgccac
    16681 atccatcatc gtggcccgca gttctgcagt gacagttttg ctcatgtcat caacgctaac
    16741 agtaatcgtt gcctcacctg actgcgttcc ggtagtaaag acagactgat acacccctgc
    16801 ttcagtttcg gtgaactcac ccagtgttgg ctttgcctgt gatttagtgg ccttcagggt
    16861 acgagtcaca atatttccag ccggtttgaa ggttagttca gtcttgatct gatctttcat
    16921 gcccgtgact ggctggccct cggcgtcgcg cagagacagc accagcggct tttgctcatt
    16981 accgttagca agcatttgaa tacggctctg accgtcaagc gttaacgccg tacgatcggc
    17041 gctcatacca gctccgctaa taaccacttc tgtctgcaca cgtttcgagg cattgccttt
    17101 gttatcgtag gcaatcgctg aaatcgcata ataattgtct ttgcctgcct gataagccgg
    17161 gagcgtcact tgccactgat tgccctgccc cgtaattttt ccgcctgcgg ccagcaaaga
    17221 cggcgcttcc cattgcacat ttttcagacc gtgagttgct ttgctgacca caagccccag
    17281 ggaaaccgtc tggccgccct tgccttcaat acgctcaggc agagcaatac ggatcacttc
    17341 agatttgcga tactcaagaa cgatattgtt attacgctca accaggtcat agcggctgcc
    17401 tgccagcatt cgacgctcgc gaatgctgtc tgtatcgagt tgtttttcca gaggttcgcc
    17461 aatccgataa ttaacttcca ggccaaagcg agtgtcattc tcaccactct tgccctgctt
    17521 atgcccggca ctcagggtca gaagaggcac tggcgtgtaa ttcacttcag cggtaatcgc
    17581 atgtggatct ttctggcgtt tatctttacc aaacagcccg acttcatcgc cataatactg
    17641 ttcatacatc aggcttgcgc caagctgcgg ccaggcaggt aaatagccct cagcacgaat
    17701 atcccagcca ttcgccgggc gttcctgata atcctcaaca tccggcgatg ttttccagcc
    17761 agaagcccgg atataaccat tggcgctcag tttcaaataa tcacgccagt attccgcacc
    17821 aacaccaatg cgggtatgac tacgggataa atcatgatcg ataaaagtat tcacccccgc
    17881 catccagtca ttttctgaaa aatgacgcca gccaaaacca atatttgact gagtacgatc
    17941 gtcggtacga tgtattgccc cctgagtgaa caacatattt gtcggtgtat cataaatcgg
    18001 ataaagcatt tccagcgaag agtccttcag cgagaaattt ttatcgacat tcagtttgac
    18061 gcgcgcagta ccgtatttcc cgagccactc ctgaatttcc tggttagctt tagcggtggc
    18121 cattccggta ataaagttac gtgtcgcatc gctatctggc tgactgctta aaaatgtccc
    18181 ggcattagcg gcaagcgacg cgacattttt ctccacgtta ttatcagcag ttaccgtagt
    18241 attttccatg ctcaaccgtg gctgaaccgc atgctgcgca cgtgctgcca ttactggcgt
    18301 aaaggtgaca gcgagtggaa aaagaacctg aacagagata tttgcccacg ccacgcagcg
    18361 ggccagaact gaataacgaa atcgtggctg tttattgtct gttttataac gtgacattga
    18421 gataatttcc tgatgaacga attaacgaac ataggcagac acaaataatg gcgtctgctt
    18481 gtcagaaaaa ccggggattt cccggtttcc ttttccacag aaaaggaaat acctttatgt
    18541 aagaaagtac accgccacct aatatatcca gtgtctgtag ccactcatca ggtggttggg
    18601 cgcgggatta tatttttatt tatgggtaaa aatttagctt tttgtgcaag tc
//
LOCUS       DAZKHK010000102        18525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKHK010000102 DAZKHK010000000
VERSION     DAZKHK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.102"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1296
                     /gene="gadB"
                     /locus_tag="ACHOS5_003642"
     CDS             <1..1296
                     /gene="gadB"
                     /locus_tag="ACHOS5_003642"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9778400.1"
                     /translation="EMRDDVAFQIINDELYLDGNARQNLATFCQTWDDDNVHKLMDLS
                     INKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNDQAVGTNTIGSSEACMLGGMAM
                     KWRWRKRMEAAGKPTNKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMI
                     EACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPF
                     VAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIG
                     TFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTG
                     RPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRR
                     GFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1452..2987
                     /gene="gadC"
                     /locus_tag="ACHOS5_003643"
     CDS             1452..2987
                     /gene="gadC"
                     /locus_tag="ACHOS5_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP9778401.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3118..4437
                     /gene="digH"
                     /locus_tag="ACHOS5_003644"
     CDS             3118..4437
                     /gene="digH"
                     /locus_tag="ACHOS5_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP9778402.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPAMTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPASRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4815..4979
                     /locus_tag="ACHOS5_003645"
                     /pseudo
     CDS             4815..4979
                     /locus_tag="ACHOS5_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            5004..7403
                     /gene="dosP"
                     /locus_tag="ACHOS5_003646"
                     /gene_synonym="pdeO"
     CDS             5004..7403
                     /gene="dosP"
                     /locus_tag="ACHOS5_003646"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP9778403.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAISPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQISDELRNVVSKPIMIDDKPFPLTLSIGISYDEGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(7593..8024)
                     /gene="osmC"
                     /locus_tag="ACHOS5_003647"
     CDS             complement(7593..8024)
                     /gene="osmC"
                     /locus_tag="ACHOS5_003647"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP9778404.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            8369..8584
                     /gene="bdm"
                     /locus_tag="ACHOS5_003648"
     CDS             8369..8584
                     /gene="bdm"
                     /locus_tag="ACHOS5_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP9778405.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            8686..8823
                     /gene="sra"
                     /locus_tag="ACHOS5_003649"
     CDS             8686..8823
                     /gene="sra"
                     /locus_tag="ACHOS5_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP9778406.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            8979..10676
                     /gene="maeA"
                     /locus_tag="ACHOS5_003650"
     CDS             8979..10676
                     /gene="maeA"
                     /locus_tag="ACHOS5_003650"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9778407.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            10810..11820
                     /gene="adhP"
                     /locus_tag="ACHOS5_003651"
     CDS             10810..11820
                     /gene="adhP"
                     /locus_tag="ACHOS5_003651"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP9778408.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            12006..12284
                     /locus_tag="ACHOS5_003652"
     CDS             12006..12284
                     /locus_tag="ACHOS5_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9778409.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            12284..12568
                     /gene="yddM"
                     /locus_tag="ACHOS5_003653"
     CDS             12284..12568
                     /gene="yddM"
                     /locus_tag="ACHOS5_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9778410.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(12797..13450)
                     /gene="fdnI"
                     /locus_tag="ACHOS5_003654"
     CDS             complement(12797..13450)
                     /gene="fdnI"
                     /locus_tag="ACHOS5_003654"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP9778411.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(13443..14327)
                     /gene="fdnH"
                     /locus_tag="ACHOS5_003655"
     CDS             complement(13443..14327)
                     /gene="fdnH"
                     /locus_tag="ACHOS5_003655"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP9778412.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(14340..17387)
                     /gene="fdnG"
                     /locus_tag="ACHOS5_003656"
     CDS             complement(14340..17387)
                     /gene="fdnG"
                     /locus_tag="ACHOS5_003656"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(16800..16802),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9778413.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            17619..18500
                     /gene="yddG"
                     /locus_tag="ACHOS5_003657"
     CDS             17619..18500
                     /gene="yddG"
                     /locus_tag="ACHOS5_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP9778414.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         4537 a         4747 c         4627 g         4614 t
ORIGIN      
        1 gaaatgcgtg atgatgtcgc attccagatt atcaatgatg aattatatct tgatggcaac
       61 gctcgtcaga acctggccac tttctgccag acctgggacg acgataatgt ccataagttg
      121 atggatttat ccattaacaa aaactggatc gacaaagaag aatatccgca atctgcagca
      181 atcgatctgc gttgcgtaaa catggttgcc gatctgtggc atgcgcctgc accgaaaaat
      241 gatcaggccg ttggcaccaa caccattggt tcttccgagg cctgtatgct cggcgggatg
      301 gcgatgaaat ggcgttggcg caagcgtatg gaagctgcag gtaagccgac taacaaaccg
      361 aacctggtgt gcggtccggt acaaatctgc tggcataaat tcgcccgcta ctgggatgtg
      421 gagttgcgtg agatccctat gcgccccggt cagttgttta tggacccgaa acgcatgatt
      481 gaagcctgcg acgaaaatac catcggcgtg gtgccgactt tcggcgtgac ctataccggt
      541 aactatgagt tcccgcaacc gctgcacgat gcgctggata aattccaggc cgacaccggt
      601 atcgacatcg acatgcacat cgacgccgcc agcggtggct tcctggcacc gttcgtcgcc
      661 ccggatatcg tctgggactt ccgcctgccg cgtgtgaaat cgatcagtgc ttcaggccat
      721 aaattcggtc tggctccgct gggctgcggc tgggttatct ggcgtgatga agaagcgctg
      781 ccgcaggaac tggtgttcaa cgttgactac ctcggcggtc agattggtac tttcgccatc
      841 aacttctccc gcccggcggg tcaggtgatt gcacagtatt atgaattcct gcgcctcggt
      901 cgtgaaggct ataccaaagt acagaacgct tcctaccagg ttgctgctta tctggcggat
      961 gaaatcgcca aactggggcc gtatgagttc atctgtaccg gtcgcccgga cgaaggcatc
     1021 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtatgacctc
     1081 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
     1141 actgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
     1201 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgaccaccc gaaactgcag
     1261 ggtattgccc agcagaacag ctttaaacac acctgataac gtttaactgt aacggtgtcc
     1321 cgaaacgaag ccgtttcggg acaatttcca aagtctgttc actggcatta gcaacggaaa
     1381 atattgttct gaatacgctt cagaacaaaa caggtgcggt tccgacagga ataccgtttt
     1441 agggggataa tatggctaca tcagtacaga caggtaaagc taagcagctc acattactcg
     1501 gattttttgc cataacggca tcgatggtaa tggctgttta tgaataccct accttcgcaa
     1561 catcgggctt ttcattagtc ttcttcctgc tattaggcgg gattttatgg tttattcccg
     1621 tgggactttg tgctgcggaa atggctaccg tcgacggctg ggaagaaggt ggcgtcttcg
     1681 cctgggtatc aaatactctg gggccgagat ggggatttgc agcgatctca tttggctatc
     1741 tgcaaatcgc cattggtttt attccgatgc tctatttcgt gttaggggca ctctcctaca
     1801 tcctgaaatg gccagcgctg aatgaagacc ccattaccaa aactattgca gcactcatca
     1861 ttctttgggc gctggcatta acgcagtttg gtggcacgaa atacacggcg cgaattgcta
     1921 aagttggctt cttcgccggt atcctgttac ctgcatttat tttgatcgca ttagcggcta
     1981 tttatctgca ctccggtgcc cccgttgcta tcgaaatgga tgcgaagacc ttcttccctg
     2041 acttctctaa agtgggcacc ctggttgtat ttgttgcctt cattttgagt tatatgggcg
     2101 ttgaagcttc cgcaacccac gtcaatgaaa tgagcaaccc cgggcgcgac tatccactgg
     2161 ctatgttact gctgatggtg gcggcaatct gcttaagctc tgttggcggt ttgtctattg
     2221 cgatggtcat tccgggtaat gaaatcaacc tctccgcagg ggtaatgcaa acctttaccg
     2281 ttctgatgtc acatgtggca ccggaaattg agtggacggt tcgcgtgatc tccgcactgc
     2341 tgttgctggg tgttctggcg gaaatcgctt cctggattgt tggtccttct cgcgggatgt
     2401 atgtcacagc gcagaaaaac ctgctgccag cggcattcgc taaaatgaac aaaaatggcg
     2461 taccggtaac gctggtcatt tcgcagttgg tgattacttc tatcgcgttg atcatcctca
     2521 ccaataccgg tggcggtaac aacatgtcct tcctgatcgc actggcgctg acggtggtga
     2581 tttatctgtg tgcttatttc atgctgttta ttggctacat tgtgttggtt cttaaacatc
     2641 ctgacttaaa acgcacattt aatatccctg gtggtaaagg ggtgaaactg gtcgtggcta
     2701 ttgtcggtct gctgacttca attatggcgt ttattgtttc cttcctgccg ccggataaca
     2761 tccagggtga ctctaccgat atgtatgttg aattactggt cgttagtttc ctggtggtac
     2821 ttgccctgcc ctttattctc tatgctgttc atgatcgtaa aagcaaagcg aataccggcg
     2881 tcactctgga gccaatcaac agtcagaacg caccaaaagg tcacttcttc ctgcacccgc
     2941 gtgcacgttc accacactat attgtgatga atgacaagaa acactaacca tgtctgaatg
     3001 gttataagac aagggagcga taattcatcg ctcccttttt cgtgcttgtt gtcgtattga
     3061 cttaaccggg ataagtacga gaatgagcgc acatctgttt accggaaacc agcacatatg
     3121 gatatctgct cccgaaacga gaaattagcg attagaagac cagcgatact ggttgcactg
     3181 gcacttttac tgtgtagttg taaaagcacg cctccagagt ccatggtgac accaccagcg
     3241 ggttcaaagc caccagccat gacgcaacaa tcgtcacaac cgatgcgtgg catctggctg
     3301 gccacggtgt ctcgcctcga ctggccaccg gtttcctcgg ttaacattag taaccccacc
     3361 agccgggcac gtgtacaaca acaggcgatg atcgacaaac tggatcatct gcaacgtctc
     3421 ggcataaaca cggtcttttt ccaggtcaag ccggatggta ctgccctgtg gccatcgaaa
     3481 attttgccgt ggtccgatct tatgaccggt aagattggtg aaaatccggg ttacgatccg
     3541 ctgcaattta tgctcgacga agcccacaag cgtgggatga aagtacacgc ctggtttaac
     3601 ccctatcgcg tatcggttaa tacgaagccc ggtactatca gggaactgaa tagcactctg
     3661 tctcaacaac cggcgagcgt ctatgtgcaa caccgcgact ggatcagaac gtccggcgat
     3721 cgctttgtcc tcgacccggg catccctgag gttcaggact ggatcacatc aatagtcgcg
     3781 gaagtggttt cccgttatcc ggtagatggc gtgcagtttg atgactattt ctatactgag
     3841 tcaccagctt cacggctaaa tgataacgaa acgtaccgca aatacggcgg cgcatttgcg
     3901 tcaaaagcag actggcggcg caacaatacc cagcagttaa ttgcgaaggt ttcgcacacc
     3961 attaaaagca ttaagccgga agtcgaattt ggcgttagcc cggcaggcgt gtggcgtaac
     4021 cgatcacacg atccgctcgg ttccgatacc cgaggcgcgg cagcctatga cgaatcctac
     4081 gctgataccc gtcgatgggt ggaacaaggg ttgctggatt acattgctcc ccaaatttac
     4141 tggccattct cacggagtgc cgcgcgttat gacgtgttgg caaaatggtg ggcggatgtc
     4201 gttaaaccga ccaggacccg cctgtatatc ggtatcgcct tctataaagt gggtgaacct
     4261 tcaaagatag agccagactg gatgattaac ggcggtgtac cggaactgaa aaagcagctc
     4321 gatcttaacg atgctgtgcc ggaaattagc ggcacaatct tgttccgtga ggactatctg
     4381 aataaaccgc agacgcaaca agcggtcagc tatctgcaaa gtcgttgggg cagttaaact
     4441 cccattctta ctttgtaaca agccggtgct ttacccactg gcttgttacc ctcttgaact
     4501 atcccactag caatcatgct taaatacgtc aaattaacca cgcaattaac cacacttagt
     4561 taaacagcca taattcccct aataaaaatg tataaatatg aaaaaacata cgatattcgc
     4621 tgtcaccgaa aaataattac gatgagctat actcttctga agaacagtag cgtagattga
     4681 aactgtccta agtcgcttat ttcttttcag tatatcttca tatttcagga gaatatggtg
     4741 cagttagtcg atctcgcacg ttgtgtttgg ttgattttgg cctgcggttt aaccataaag
     4801 gtcgacatta ttttaaagcc gcaaacggcg aggatattgc cctctcactt tccatcggtg
     4861 ccgccatgtt taatggtcat cctgactatg agcgcctcat tcaaatagcc gatgaagctc
     4921 tgtatatcgc caaaagacga ggtagaaacc gtgttgaact ctgtaaagcc agtctttaga
     4981 tgcgccagga tgcagaggta atcatgaagc taaccgatgc ggataatgcc gccgatggca
     5041 tttttttccc cgcccttgag caaaatatga tgggcgcggt gttaattaac gaaaatgatg
     5101 aagtgatgtt tttcaacccc gccgcagaga agctctgggg gtacaaacgt gaagaagtca
     5161 ttggcaataa cattgatatg ctgattccgc gggatttgcg tcctgcgcat cctcaataca
     5221 ttcgtcacaa ccgtgaaggt ggtaaagctc gcgtcgaggg gatgagtcgg gagctgcagc
     5281 tggagaaaaa agacggcagt aaaatctgga cccgttttgc gctatcgaaa gtgagcgccg
     5341 aggggaaagt ttattacctg gcgctggtac gggatgccag cgtagaaatg gcgcaaaaag
     5401 aacagacccg acaattgatt attgccgttg accatctcga ccgaccggtg attgtcctcg
     5461 atccggaacg ccatattgtg cagtgcaatc gcgcatttac cgaaatgttt ggttactgca
     5521 ttaacgaagc cagcggtatg cagcccgata cactcctgaa cattccagaa ttccctgctg
     5581 ataaccgtat tcgtttacag cagttgctat ggaaaaccgc ccgcgatcag gacgaatttc
     5641 tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc ctctatcagc ccggtttatg
     5701 acgtgctcgc gcatctgcag aacctggtaa tgacattctc ggatatcacc gaagaaaggc
     5761 aaatccgcca gcttgaaggc aatattctcg ccgccatgtg cagcagcccc ccatttcatg
     5821 aaatggggga aatcatttgt cgtaacatcg aatctgttct caacgaatcg catgtttcgc
     5881 tgttcgcgct gcgcaacggg atgccgatac actgggcgtc atcttcgcac ggtgcagaag
     5941 ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg cgatggcgcg cctgcgggga
     6001 tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa cgcgtggcag
     6061 atatcagcca gcatatggcc gcgctggcgc tggaacagga aaaaagccgt cagcatattg
     6121 aacaactcat ccaatttgat ccaatgaccg gtctgccaaa tcgcaataac ctgcacaatt
     6181 acctcgatga cctggtcgac aaagccatct ctccggtggt gtatctcatc ggtgttgacc
     6241 atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca ttgctggaag
     6301 tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt attgaaggtg
     6361 cgtcttttgt cctcgtgagc ctggaaaacg atgtcagtaa cattacccag atctccgatg
     6421 agctacggaa tgtggtcagc aagccgataa tgattgacga taaaccattc ccgctcacct
     6481 tgagcattgg catcagctac gacgagggta aaaaccgcga ttacctgctc tccactgctc
     6541 acaatgcaat ggattttatt cgcaaaaatg gcggtaacgg ctggcagttc ttcagcccgg
     6601 cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctaaaa gaagcgatta
     6661 gcaataacca acttaaactg gtttatcagc cgcaaatctt cgcagaaacg ggtgaactgt
     6721 acggcatcga agcccttgct cgctggtacg atcccctgca tggtcatgtg cccccttcac
     6781 ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc tgggtcatcg
     6841 cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc ccggcgttat
     6901 ccgttaactt gtctgcgctg cactttcgca gtaatcagct gcctaatcag gtatctgatg
     6961 caatgcacgc ctggggtatt gacggccacc agctgacggt ggaaatcacg gaaagcatga
     7021 tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgcgat atgggcatcg
     7081 gtttatcggt agatgatttt ggtacaggct tttccggatt atcccgctta gtcagtcttc
     7141 cggtaacgga aatcaaaatt gataaaagtt ttgtcgatcg ttgtctgacc gaaaaacgca
     7201 tccttgcctt actcgaagcc attaccagca ttgggcaaag cctcaattta accgtcgtgg
     7261 ctgaaggcat cgaaaccaaa gagcaatttg agatgctacg caagatccac tgtcgcgtta
     7321 ttcagggata tttcttttcc cgccccctgc ccgccgaaga aattccaggc tggatgagca
     7381 gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg tttttattta
     7441 acgtgacact gtcacttaaa tactgtgatt tccgctacgt ttagttggac aacgacaacg
     7501 catagccatg tcagaacaat cccttctcag cctgatgtga ttgtgctgac gcatcaggca
     7561 ttcacgcgcc tgacgcgtca tccggcaacg ctttacgatt tcaactggta atccagcgta
     7621 atttccgctt tcagtacctg agaaaccggg catcctgctt ttgctttctg gataatgccg
     7681 tcaaaggtgg aggcatcaat acccggcacc gcaacttcgc tcttcagtgc gattttagta
     7741 atcgcaaaac cggcatccac tttatccagc gagacatcgg cggtggtatc aatcgatgtt
     7801 ggcgtgaatc ccgcttcccc cagcattaat gaaagcgcca ttgagaaaca tgcggcatgt
     7861 gctgcgccaa tcagttcttc agggttggtt cctttttcgc cttcaaaacg cgtgttaaat
     7921 ccatacggct gttggttcag cacgccgctc tcagtagata ctgttccctt cccgcgtttg
     7981 atatcgcctt cccagtgtgc ctgacctttc ttatggattg tcattgttgc tctcctgtgg
     8041 gcttaaaaag gaaattatag actacgtgag aaacagcacg aggataagca ggataatccg
     8101 aataaaatcc ggcgaaaata taattcctgc acttaaatta aatttccaca atagataacc
     8161 ggaaatatat aactcaagag atattgcatc aatatcaatc aactcatcac aataataata
     8221 cctatcaata tcatggagtt aaaatataaa aatcatcaac caggactaat cttaacaacg
     8281 aagcggcaaa tatttgccat gctgaatgtg ctttataaat ctgcgatccg tagcagacac
     8341 cataaataca cagacacgga gaatcactat gtttacttat tatcaggcag aaaattcaac
     8401 agcagaaccc gctctggtta atgccattga gcaaggcctt cgggcagagc acggtgttgt
     8461 cactgaagat gacattctca tggagctgac caaatgggtg gaagcatctg ataacgacat
     8521 cctcagtgat atctaccagc aaacgatcaa ttatgtggtc agtggtcagc accctacgct
     8581 ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta
     8641 cggcgatgtt gtccttaaaa ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt
     8701 caggcacgtc atattcttgg actggaccat aaaatttcta accagcgcaa aatagttacc
     8761 gaaggtgaca aatccagcgt ggtaaataat cccaccggca gaaaacgccc cgctgaaaag
     8821 taattcataa ccatcagtcc tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt
     8881 ttgtttttat ctgctttata cttggtgccg acgccctggc ggtaatgcaa agacgataaa
     8941 agcccccagg gatggatatt caaaaaagag tgagtgacat ggaaccaaaa acaaaaaaac
     9001 agcgttcgct ttatatccct tacgctggcc ctgtattgct ggaatttccg ttgttgaata
     9061 aaggcagcgc cttcagcatg gaagaacgcc gtaacttcaa cctgctgggg ttactgccgg
     9121 aagtggtcga aaccatcgaa gaacaagcgg aacgagcatg gatccagtat cagggattca
     9181 aaaccgaaat cgacaaacac atctacctgc gtaacattca ggacactaac gaaaccctct
     9241 tctaccgtct ggtaaacaat catcttgatg agatgatgcc agtcatttac accccaaccg
     9301 tcggcgcagc ctgtgagcgt ttttctgaga tctaccgccg ttcacgcggt gtgtttatct
     9361 cttaccagaa ccgccacaat atggacgata ttctgcaaaa cgtaccaaac cataatatca
     9421 aagtgattgt ggtgactgac ggcgaacgta ttctggggct tggtgaccag ggcatcggcg
     9481 ggatgggcat tccgatcggt aaactgtcgc tctataccgc ctgtggcggc atcagtccgg
     9541 cctataccct tccggtggtg ctggatgtcg gcaccaacaa ccaacagcta cttaacgatc
     9601 cgctgtatat gggctggcgt aatccgcgta tcactgacga cgaatactat gaattcgttg
     9661 atgaatttat ccaggctgtg aaacaacgct ggccggacgt actgttgcag tttgaagact
     9721 tcgcacaaaa aaatgcgatg ccgttactta accgctatcg caatgaaatt tgttctttta
     9781 acgatgacat tcagggcacc gcggcggtaa cagtcggcac actgatcgca gccagccgcg
     9841 cggcaggtgg tcagttaagc gagaaaaaaa tcgtcttcct tggcgcaggt tcagcgggat
     9901 gcggcattgc cgaaatgatc atcgcccaga cccagcgcga aggattaagc gaggaagcgg
     9961 cgcggcagaa agtctttatg gtcgatcgct ttggcctgct gaccgacaag atgccgaacc
    10021 tgctgccttt ccagaccaaa ctggtgcaga agcgcgaaaa tctcagtgac tgggataccg
    10081 acagcgatgt gctgtcactg ctggatgtgg tgcgtaatgt aaaaccagat attctgattg
    10141 gcgtctcagg acagaccggg ctgtttacgg aagagatcat ccgtgagatg cataaacact
    10201 gtccgcgtcc gatcgtgatg ccgctgtcta acccgacgtc acgcgtggaa gccacaccgc
    10261 aggacattat cgcctggact gaaggtaacg cgctggtcgc cactggcagt ccgtttaatc
    10321 cagtggtatg gaaagataaa atctacccta tagcccagtg caacaacgcc tttattttcc
    10381 cgggcatcgg gctgggtgtt attgcttccg gcgcgtcacg tatcaccgat gagatgctga
    10441 tgtcggcaag tgaaacgctt gctcagtatt cgccgctggt cctgaacggc gaaggtctgg
    10501 tactaccgga actgaaagat attcagaaag tctcccgcgc aattgccttt gcggttggca
    10561 aaatggcgca gcagcaaggc gtggcggtga aaacgtctgc cgaagctctg caacaggcca
    10621 ttgatgataa tttctggcac gccgaatacc gcgactaccg ccgtacctcc atctaagcct
    10681 gcgcccggta gtgaaggcta ccgggcaatt ttcctctccc tttttcagat ctcatccata
    10741 ctgggtagtg gcgaataaat ctcatttgcc tcacctgcta tgcagaacat catccgaaaa
    10801 ggaggaacta tgaaggctgc agttgttacg aaggatcatc atgttgacgt tacggataaa
    10861 acactgcgct cactgaaaca tggcgaagcc ctgctgaaaa tggagtgttg tggtgtatgt
    10921 cataccgatc ttcatgttaa gaatggcgat tttggtgaca aaaccggcgt gattctggga
    10981 cacgaaggta ttggtgtggt ggcagaagtt ggccctggtg ttacttcatt aaaaccaggc
    11041 gatcgtgcca gcgtggcgtg gttctatgaa ggatgcggtc attgcgaata ctgtaacagt
    11101 ggtaacgaaa cgctctgccg ttcagttaaa aatgccggat acagcgttga tggcgggatg
    11161 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
    11221 gcggcggcca gcagcattac ctgtgcgggg gtcactacct acaaagccgt taagctgtca
    11281 aaaattcgtc caggacagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
    11341 ctgcaatacg cgaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgaa
    11401 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
    11461 gccgccagaa ttgtgcagga gaaagccggt ggcgctcacg cagcggtggt gacagcggta
    11521 gctaaagctg cgtttaactc ggcggtggat gctgtccgtg caggtggtcg tgtcgtggct
    11581 gtcggtctgc cgccggagtc tatgagcctg gacatcccac ggctggtgct ggatggtatt
    11641 gaagtggtcg gttcgctggt cggcacgcgt caggatctaa ccgaagcctt ccagtttgcc
    11701 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccata
    11761 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcgctaa
    11821 gaggtctttg ctgcgactgc catgttcggg tcgcagcctg ttttgcgaga cgctttccgc
    11881 aatttcactc ttcttctttt cctcccccat ctttcccctc acaattcgcg aaaacgtagc
    11941 gaaatttgtt gcgctgtacg gaacaatagt gatagtgttc ctttcgaggg aacaaggatg
    12001 aagagatgat aatgaatttc agacacaagg gattgcgtga cttgtttctt ctcggcaaaa
    12061 cctccggcgt tataccgacg caagtcaaac gattacgcca ccgactcgct gtgattgatg
    12121 cagcatgctg tcttgctgat atcgatatgc ccggttaccg actacatccg ttaagcggcg
    12181 atcgcgatgg aatttgggcg atatctgtct cgggcaactg gcgaatcaca tttgaattcg
    12241 tcaatggcga tgcatacata ctggattacg aggactatca ctgatgaaaa tggccaatca
    12301 tccccgcccg ggggacatta ttcaggaatc actggacgaa cttaatgtca gcctgcgcga
    12361 gtttgccaga gcaatggaaa ttgcgccctc aacggcaagc cgattgctga ccggaaaagc
    12421 agctttgacg ccagaaatgg caataaaact ttccgtggtg atcggcagtt cgccgcaaat
    12481 gtggctgaat ctgcaaaatg cctggagtct ggcagaggcg gaaaaaacgg tggatgtgtc
    12541 gcgactgcgc cgtctggtta cgcaataaaa aaatggcacc ttataaagcg ccatttgaaa
    12601 ctgatgacaa acgcaacatt gcccgatgcg ctacgcttat caggtctaca cgttcactgc
    12661 aatctattgg atttgaacgg tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
    12721 aacaaagtgc acgttgtcta caataaaaaa atggcgcctt ttacagcgcc attttcagct
    12781 taaagctcaa aagagattat aacccttctt cactctcttt tttcgcttct gccttctcga
    12841 tttctcgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
    12901 tcatcccttt aatcgatcct ttcacccaga atgccatata catatggatc aggatggcgt
    12961 ggatcaggat gatacccgca gccgcatgga tcagcaggct ataacggaca acctgtatcg
    13021 ggaagtactg tgcaaagtac ggacgccaga taatcactcc ggtcaccagc agcacgaaaa
    13081 tcatgctcat gattgaccag aacatcatct tttgcccggc gttgtactta ccgacatccg
    13141 ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt
    13201 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagc gcgacgaaaa
    13261 tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
    13321 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
    13381 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
    13441 acttactcat gatttccctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
    13501 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
    13561 gcgcctttcc acaggcttac cgaggtatcg atcttcggat ctttcggcag accatgatac
    13621 agctccggct gatcggcatg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
    13681 gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgctac gcgctgttcc
    13741 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
    13801 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
    13861 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
    13921 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
    13981 ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta
    14041 cggatcagcc actccagctt gccgttctgt tcggtttcgc taaagcgcat caccgtccag
    14101 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
    14161 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
    14221 acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
    14281 gagttagttg cggacctttt gataatgtcc tgcgtttcca tagccatctg ttcgccccct
    14341 tacgccttct cgatgtttac taagaacgct ttatattccg gcgtttgcga gtttgcatca
    14401 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
    14461 cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga
    14521 cggcgcgtta ccacagccac cgcgcggata aagccacgct tgctggagac agtgacacga
    14581 tcgccattgg caatgccttt cgccgccgcc agcgtttcgc tgatttccac aaactgttcc
    14641 ggctgagcaa ttgcgttgag caatgcgtgc ttggtccagg tgtggaagtg ctccgtcaga
    14701 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag tgcgtcttgt
    14761 tcatacagac gaacaaccgg gttagacacc acgttcggat gcaacgggtt agtgcccagc
    14821 ggcgtttcaa ttggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
    14881 aacaggcgtc ccatcccttc cggctgcatg ataaacggcc cggttggcgt acccggtgcg
    14941 gcattgccga agtccggaat atcgttaccc gtccacttgc taccgttcca ctggatcagc
    15001 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg
    15061 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
    15121 gagttatcgc ggttagccat ctggttgccc tgctcggtcc agctaccggt gtagatccag
    15181 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
    15241 ttcttcgcaa tcagcacgcc attagcgtca tagagatctt ccagcgcata gccgttgttc
    15301 tctttcgcca cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
    15361 atcagcggtt ctacgccttt accaccttcg gcctggtaca gctcgcgcag atgatggtag
    15421 atacccgcca gaatttcgcc gtcgttacgt gcttcgcccg gcgcgtcctg acctttccag
    15481 tgccactgca accagcgacc ggagttagcg atagaaccat cttcttcagc aaagcaggtc
    15541 gaaggcagac ggaatacttc agtctgaata gatgccggat cgacatcgtt cgactcgccg
    15601 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
    15661 agcttgctca ggcagctcac cactttgttt ttgtccggga aggacgcaac cgggttaaag
    15721 ccctggcaga aataaccggt gactttacct tcatccatca tgttgaaata cttgatgacg
    15781 tcgtaggtct ggtcccactt cggcagccag tcgtagcccc agttgttctc tttctgcgcg
    15841 gcatcgccgt agaaagattt catcaggcta acgaaaaact tcggatagtt gctccagtag
    15901 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
    15961 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag caggcctaag
    16021 tcagtcaggc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
    16081 cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
    16141 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
    16201 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccgca gatgttttct
    16261 actacgtccg gcgtgtagcg ggaaacgtgc gctttcagca gattccatac acagcgcgga
    16321 tgagtcagcg tgtcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
    16381 gacttatcgt attgacgttt ttcagcgtcg tagccgctga acagaccgtc ttcgaaagca
    16441 aaatcatcac gcaccagcag gctggcgttg gtgtaatgct taacgtattc ggcgttgatt
    16501 ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa
    16561 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg atcgacaacg
    16621 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
    16681 gcttcagcag cgttaccgcc catcaccatt acgacgttag cgtttttgat atccacccag
    16741 tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
    16801 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttttgagtc
    16861 agcatcccgg tttcgttgct ggcaccggaa gcacacagca taccggtaga aagccaacgg
    16921 tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc ggctttcatc
    16981 agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
    17041 ggcgcacgat attccgggta gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg
    17101 gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
    17161 taaatcgctt ctctggcgtt tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa
    17221 cctacggaac agtatgtgca ggtgttacgg atctctttcg cgcgtaataa tttgtagttt
    17281 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ctgctactgt tgttcccgcc
    17341 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
    17401 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
    17461 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
    17521 tagcggtaga aaaacgcccc actgcctgac agggcagtta aaaaaatgct ataaaattca
    17581 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
    17641 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
    17701 gtgagggact cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
    17761 taatcttcac ggttggattt ccgcgtattc ggcagatccc gaaaggctat ttactcgccg
    17821 ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg tatgcggcga
    17881 cccgtcatca ggcgattgaa gtggggatgg tgaactatct atggcccagc ctgacaattc
    17941 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
    18001 tagccctggt cggcgtctgc tgggtgttag gcggtgacaa tgggttacat tatgatcaaa
    18061 ttattaataa tatcaccacc agcccattga gttatttcct ggcgttcatt ggggcgttta
    18121 tctgggcagc ctactgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
    18181 tttttgtcct gctaacggga gcaagtctgt gggtttacta ttttcttacg ccacaaccag
    18241 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
    18301 ttgcttatgc tgcatggaat gtcggtatat tacatggcaa cgtcaccata atggcggtag
    18361 gttcgtattt tacgcccgtc ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
    18421 tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc
    18481 tggcgacacg tcgtggctaa aataattgtc gggtctacgg cccgg
//
LOCUS       DAZKHK010000103        17950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKHK010000103 DAZKHK010000000
VERSION     DAZKHK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.103"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1104
                     /locus_tag="ACHOS5_003658"
     CDS             <1..1104
                     /locus_tag="ACHOS5_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP9778415.1"
                     /translation="NEQYPDADAVIINGDLTEFGHADQWRDFESIVSRLKIPYYYGLG
                     NHDIYNNYNDCWENNCVIRSVTKLFHHVNSKDNISDFDVNYTHGYVFPEVKETIKGSL
                     SYSVDFGNVLLIQLNDYDKGKNPLKINQYTSGAWGGGAMRHEIDRYQDAEYSWLERQL
                     YSAYKNNQVVIVNQHRFDADAGNLKKLLDKYNVQLRFAGHHHNWIGEKKGDFRLSGSS
                     ALGTYLKVDVDTSKKTAKVYKGVNNTATPELIETISLEPPKGNITPPPPGPVYLRVKT
                     SGGYEAFVSLVYRTKDGQQKKINSGKLLAGNSWEYNVPGGSTIEYLEARNNTGLVWEP
                     QRRIFRANNIRNDACFSTWGTTLNSAWKQVSCR"
     gene            1190..1972
                     /locus_tag="ACHOS5_003659"
     CDS             1190..1972
                     /locus_tag="ACHOS5_003659"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9778416.1"
                     /translation="MFPYICVVIGLIAGSFLNVVIRRYPVMLRQQIAEFQGETQNVQP
                     GINLIRPRSHCPHCRKSIRIRDNIPLVSWLILKGRCRDCHGKISLRYPLVELLTALLF
                     LLAGIVWPDSGWGVAVMILSAWLIAASFIDLEHQWLPDIFTQGVLWTGLIVAWGGLSP
                     LSLHDAVSGGLAGFVSFYTLRWLAGVVLRREALGMGDVLLFAGLGSWTGPLLLPDVAL
                     VASFCALIYAITTGKYASTVPFGPFLSLGGMVMLYTGIDVML"
     gene            2013..2423
                     /gene="gspS2"
                     /locus_tag="ACHOS5_003660"
     CDS             2013..2423
                     /gene="gspS2"
                     /locus_tag="ACHOS5_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFP9778417.1"
                     /translation="MSEKQIPGKSVIPVLLSIVASLSGCAGYQSEADMLAKEQARNIS
                     RNLPVKSANYTLISAQNSGALIKMVVISEGTGQLTLNPDVFLTQFQYQICTDPAMKAM
                     MNKGVFYIIKINEMRTGNQYQRKLDRAACGIKKL"
     gene            2523..3386
                     /gene="gspC"
                     /locus_tag="ACHOS5_003661"
     CDS             2523..3386
                     /gene="gspC"
                     /locus_tag="ACHOS5_003661"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9778418.1"
                     /translation="MLKKWLLKFIHYIFCIILFFIFIKIIISAWHYATFSVVQVKHSP
                     VQHKERQQNAGMTGSECCQLISQQNWFGEYHPVTVPEHSPLTLMVKEKLPDIVLRGVA
                     SGARPGVVLEEKGRQQFYLQGERLSSGDAVIERIFPEQVMLRYQDNVIYLTLTDEEGA
                     PEPEKKDEQPEISSPPETSAVADTFSRLPPAVQQALAIDPQKFFEYIRFTPVYKDGVA
                     GYAVMPGGDRSLFDISGLKAGDIAVALNGQDFTQPGATSIFMQQIYSMSIVRLTILRN
                     GERHNISVALR"
     gene            3446..5503
                     /gene="gspD"
                     /locus_tag="ACHOS5_003662"
     CDS             3446..5503
                     /gene="gspD"
                     /locus_tag="ACHOS5_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP9778419.1"
                     /translation="MLRARREDDRQKKITGIKKKKYLLSLILMAALCSPSSRAEENTF
                     TASFKDTDLKSFIETVGANLNKTIVMGPDVQGKVSIRTITPLNERQYYQLFLNLLEAQ
                     GYAVVPVENNVLKVVKSGAAKTEPLPLTGEGHENYVGDEMVTRIVPVRNVSVRELAPV
                     LQQMTDLDGSGSIVNYEPSNVIMLTGRASVVKRLTEVIQRIDREGNRTEEVIPLGNAS
                     ASEIARVLESLTRNSSENQPATLKSQIVADERTNSVIVSADPATRDKIRRLIRRLDSE
                     MERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTSAKEEAEGTTGSRRDVVSIAASKHSN
                     ALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIAEVAEGSNINFGVQWMSKDTGFMQ
                     FANGTQIPIGSLSMAVSQAKPQKGSTVISENGATTINPDTEGDLSRLSQLLSGFSGTA
                     VGVVKGDWAALVQAVKNDSGTNVLSMPSITTLDNQEAFFMVGQDVPVLTGSAVGENNS
                     NPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTVL
                     ANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTIL
                     RDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNAG
                     RTR"
     gene            5503..6996
                     /gene="gspE"
                     /locus_tag="ACHOS5_003663"
     CDS             5503..6996
                     /gene="gspE"
                     /locus_tag="ACHOS5_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9778420.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLDAPFTLRQIDEAEFEQRLNTVWQRDSSEARQLMEDLGSTEDFFTLAEDL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSHELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCSQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELLVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTLEA"
     gene            6996..8219
                     /gene="gspF"
                     /locus_tag="ACHOS5_003664"
     CDS             6996..8219
                     /gene="gspF"
                     /locus_tag="ACHOS5_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9778421.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMHLRWDKTLLRLPVIGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAAERVREGSCLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVSQEREFDTQVGLVLGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            8236..8691
                     /gene="gspG"
                     /locus_tag="ACHOS5_003665"
     CDS             8236..8691
                     /gene="gspG"
                     /locus_tag="ACHOS5_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9778422.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMVDSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGMFDVYTLGADGQENGEGAAADIGNWNLQELQ"
     gene            8695..9258
                     /gene="gspH"
                     /locus_tag="ACHOS5_003666"
     CDS             8695..9258
                     /gene="gspH"
                     /locus_tag="ACHOS5_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9778423.1"
                     /translation="MPERGFTLLEIILVIFLIGLASAGVVQTLATSSEPPAKKVAQDF
                     LIRFEQLKDKAVTEGLAVGVLIEPSSYQFMQRRHGQWLPVSAPRLSAQVTVPEQVQMV
                     LQPGSDIWQKEYVLELQRHRLTLHDIELELQKETKKKRPQIRFFPFEPATPFTLRFYS
                     AEQNECQAVKLAHDGALSLSQCDERMP"
     gene            9255..9626
                     /gene="gspI"
                     /locus_tag="ACHOS5_003667"
     CDS             9255..9626
                     /gene="gspI"
                     /locus_tag="ACHOS5_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9778424.1"
                     /translation="MKRGFTLLEVMIALVIFSLAVMAVLQIASGTLSNQQVLEEKTVA
                     GWVAENQTALLYLMTHEQRAIRHQGESDMAGSHWYWKTIPLKTDNALLQAVDIEVSRH
                     EDFSPVIQSRRAWFSAVGGQQ"
     gene            9623..10228
                     /gene="gspJ"
                     /locus_tag="ACHOS5_003668"
     CDS             9623..10228
                     /gene="gspJ"
                     /locus_tag="ACHOS5_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9778425.1"
                     /translation="MKRILAGFTLLEMLIAIAIFASLALMTQQVTNGVIRANSVVAGH
                     DKKLHIVQQAMSFLTHDLTQMMPRPIRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     INPLMRLPRSNLLTVGYRIRNGYLERLVWPLTDAADSVEPAIQKLFPVDSLRLQFHDG
                     TGWLEDWSSQQTIPAAVRMILHSPQWGDIERIWLLHGALSS"
     gene            10225..11190
                     /gene="gspK"
                     /locus_tag="ACHOS5_003669"
     CDS             10225..11190
                     /gene="gspK"
                     /locus_tag="ACHOS5_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9778426.1"
                     /translation="MNGFLPRRGMALLMVLLLLAVMASVCTGLSHRISRQTDRMRSLQ
                     DYQQATWYAVSAESLALSILNENLKNEEHTPVYSWSIGSHLFPLSQGKIAVTLRDAQT
                     CFNLNALAQPAKVSGAPGRQQLITLISRLDVPVQRAVQITESLQVFTGGGRDGQAQQE
                     SEYLTRPVPFWPAKQPLTDISEIRVVQGMDAALYQKIKPLVCALPMSRQRININSLDV
                     SQSVILEALFGPGLSPVQARALLQQRPGKGWRNVGQFLASPYLKRVDENVRKQVGEFL
                     TVSSHYFWLRSDITVNETELTMNSLIARTGSQHFSVLWHQTGESE"
     gene            11187..12365
                     /gene="gspL"
                     /locus_tag="ACHOS5_003670"
     CDS             11187..12365
                     /gene="gspL"
                     /locus_tag="ACHOS5_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9778427.1"
                     /translation="MSSMPEIFFPLCASDPVRWQRRTADAEHGIWPDIADEQLQQWLQ
                     TDAIRLYIPGEWISVWQAELPDVPRWQIPTILPVLLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQHHMRNIAQWLQENGITSATVAPDWMSIPGGYMAGDAQRVICRIDECRGWS
                     AGLALAPAMFRAQLNEQDLPLSLTVVGIAPEKLSAWADADAERLTITALPAINTSGEP
                     EGNLLSGQWQPRVSYRKQWARWRVMVLPVLLILVALAVERGVTLWSVSEQVAQSRAQA
                     EKLFLTLFPEQKRIVNLRSQVATALKKYRPQTDDTDLLAELSALASTLKSAALSDIEM
                     RGFTFDQNRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            12367..12903
                     /gene="gspM"
                     /locus_tag="ACHOS5_003671"
     CDS             12367..12903
                     /gene="gspM"
                     /locus_tag="ACHOS5_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP9778428.1"
                     /translation="MLRGKFVHRFQQWCERQLNNAEQWLVQHLEGRSSREKGMLLAAA
                     VFLFSAGYYVLIWQPLSERIEQQKTMLQQLVAMNARLKSAAPEIIAARKSTTTTPAQV
                     SQIISDSALSRGIAITWMDARGEYIQVRTGAVVFNDLLEWLNELRDEYALGVAQLDVK
                     QAELSGMVNIQRLELTRR"
     gene            13245..13493
                     /locus_tag="ACHOS5_003672"
                     /pseudo
     CDS             13245..13493
                     /locus_tag="ACHOS5_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
     gene            13558..13863
                     /locus_tag="ACHOS5_003673"
                     /pseudo
     CDS             13558..13863
                     /locus_tag="ACHOS5_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13918..14232
                     /locus_tag="ACHOS5_003674"
                     /pseudo
     CDS             13918..14232
                     /locus_tag="ACHOS5_003674"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            14557..14706
                     /locus_tag="ACHOS5_003675"
                     /pseudo
     CDS             14557..14706
                     /locus_tag="ACHOS5_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14881..15270
                     /locus_tag="ACHOS5_003676"
                     /pseudo
     CDS             14881..15270
                     /locus_tag="ACHOS5_003676"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            15780..16031
                     /locus_tag="ACHOS5_003677"
                     /pseudo
     CDS             15780..16031
                     /locus_tag="ACHOS5_003677"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
     gene            16065..16246
                     /locus_tag="ACHOS5_003678"
                     /pseudo
     CDS             16065..16246
                     /locus_tag="ACHOS5_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460479.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            16430..17134
                     /locus_tag="ACHOS5_003679"
     CDS             16430..17134
                     /locus_tag="ACHOS5_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778429.1"
                     /translation="MSIKDKAQHGGLNPKTKLCHHSKWITFLSIVFCTLVTGFFWLFI
                     FYLILRPSFYSYIGFADNFVLKKPDVISDPKTLQNIGKMVTDGTLLSLDDFWSFQSNL
                     YQTIITVLIALNGIVAAFSFLIIKNSSTAKAKEEARDIAVIEVNQYVESSKFEKEIKA
                     ILYEKIEPVQFDLLAQISSIDDIKLSQQEHNQSFEKLEQELDGLKRNLKLIAESISLK
                     DTSDEDGGNFILGRRT"
     gene            17135..17677
                     /locus_tag="ACHOS5_003680"
     CDS             17135..17677
                     /locus_tag="ACHOS5_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFP9778430.1"
                     /translation="MAFMTKKLKSSGVISGQDIDMTTASELIVFAKKNNIETSPLDVS
                     RLTSLLGITLRLEPMKGEESGSLKQGKSGNWIMTVNSLHHPHRQRFTIAHELGHFIRH
                     TLNRNSFTDTVFFRNEETNKMEAEANRFAAELLMPENEFSKFIEEKSNQVADIAAHFQ
                     VSSMAVRIRAQQLGFSGHNL"
     gene            17681..>17950
                     /locus_tag="ACHOS5_003681"
     CDS             17681..>17950
                     /locus_tag="ACHOS5_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFP9778431.1"
                     /translation="MKKFNYFPIMKTRDAELKAMSKLSSGVFDHILPIYELTKSRKAK
                     IAPDGDIHRRMLTIKDIQGERPFILDLTSNEKYINDQIRQLVDEKD"
BASE COUNT         4637 a         4092 c         4858 g         4363 t
ORIGIN      
        1 aatgaacaat accctgatgc tgatgccgtt atcattaatg gtgatttgac ggaatttggt
       61 catgccgatc agtggcggga ttttgagtca attgtcagca gactaaaaat tccttattat
      121 tacggtctgg gcaatcatga tatatacaat aattataatg attgttggga gaataattgt
      181 gtaattcgga gtgttactaa gctgtttcat catgtaaaca gcaaagacaa tatctctgat
      241 tttgatgtga attatacgca cgggtatgtc ttcccggaag ttaaagaaac aataaaagga
      301 tctttgtcat attctgtcga tttcggaaat gtattactca ttcagctcaa tgattatgat
      361 aaagggaaaa atccattaaa aataaatcaa tatacttctg gtgcctgggg cgggggagcc
      421 atgcgacacg aaatagacag gtatcaggat gcagaatatt catggcttga acgacagctc
      481 tactccgcat ataagaataa tcaggtggtc atcgttaacc agcatcgctt tgatgcagat
      541 gccggaaatc tgaagaaatt acttgataaa tataatgtgc agttgcgttt tgccgggcat
      601 catcataact ggattgggga aaaaaaaggc gactttcgac tttctggttc gagtgcttta
      661 ggtacctacc ttaaagtgga tgttgatacc agtaaaaaga ccgcaaaagt ttataagggt
      721 gtgaataata cagccacacc tgaactgatt gaaaccattt ctctggagcc accgaaaggg
      781 aacatcacac ctccgcctcc ggggccggtt tatcttcgcg tcaagacgtc cggcggttat
      841 gaggcttttg tttctctggt atacagaacc aaagacgggc aacagaaaaa aattaattcc
      901 ggtaaattac ttgccggtaa ctcctgggaa tacaatgttc ctggcggttc gacgattgaa
      961 tatctggaag caagaaataa taccgggctg gtctgggaac cccagagaag aatattcaga
     1021 gccaataata tcaggaatga tgcctgtttt tccacctggg gaaccacgct gaactctgcc
     1081 tggaaacagg ttagttgtcg ctgataagga actatcttta tttatattta caggttcctg
     1141 ccggtgcagg gacctgtaat aaatattatt ttttatgaga tattgaacat tgtttcctta
     1201 tatttgtgtt gtgataggtc ttattgccgg cagttttctg aatgttgtga tccgacgcta
     1261 tcctgttatg ctccggcaac agatagctga atttcagggc gaaacgcaga atgtgcaacc
     1321 cggaataaac ctgatacgcc cacgttcgca ctgcccacat tgccggaaga gtatccgcat
     1381 tcgtgacaac ataccgctgg tttcctggct gatacttaaa gggcgatgcc gagactgcca
     1441 tggaaaaatc agtctgcgtt acccgctggt tgaattattg acggcactat tatttttact
     1501 ggcaggcatt gtctggccgg acagcgggtg gggtgtggct gtcatgatat tatctgcctg
     1561 gctgattgct gcaagcttta ttgatcttga gcatcagtgg ttacctgata ttttcactca
     1621 gggcgtgttg tggaccgggc ttattgtggc atggggggga ctgagtcctt tgtccctgca
     1681 tgatgctgtt agcggggggc tggcggggtt tgtgtctttt tatactctcc ggtggctggc
     1741 cggtgttgtc ctgcgcaggg aagcgctggg tatgggcgac gttttgttgt ttgccggcct
     1801 ggggagctgg accggtcctt tgttactgcc ggatgtggcg ctcgtggcgt cattctgtgc
     1861 tctgatatat gccatcacga cagggaaata tgcaagtaca gtgcctttcg gcccctttct
     1921 gagtctggga ggaatggtga tgttatatac aggcattgat gtaatgcttt aagcgttatt
     1981 ttatttactg agcatgtgga ggggagctcg ttatgtcaga aaaacagata ccggggaaaa
     2041 gcgttattcc ggtactgctg agtattgttg catccttaag tggatgtgcg ggataccaga
     2101 gtgaagcgga catgctggca aaagaacagg cacgcaatat cagcaggaat ctgccggtta
     2161 aatcggcgaa ttatacactg atatcagcgc agaacagtgg agcgctgata aaaatggttg
     2221 ttatcagcga aggcaccggg caactcactc tgaatccgga tgttttctta acgcagtttc
     2281 agtaccagat atgcacggat ccggcaatga aggcgatgat gaataaaggc gttttttata
     2341 taataaaaat taatgaaatg cgtacaggta atcagtatca acgaaaactt gaccgtgccg
     2401 cctgtggaat aaagaaatta taatatcaga tatgcaataa taagacatat tattgataat
     2461 gcaggtattc atgttattaa gttgcaggac agagttgtgg atttccgggt gttgttgctt
     2521 atatgcttaa gaagtggctg ttaaaattca tacattatat tttctgtata atactgtttt
     2581 ttatttttat aaaaatcatc atttcagctt ggcattatgc cactttttca gttgtgcagg
     2641 tgaaacattc cccggtacag cataaagaaa gacaacagaa cgccggaatg acggggagtg
     2701 agtgctgcca gttaatcagc cagcaaaact ggtttggtga gtatcacccc gttactgtgc
     2761 cggagcacag cccgttgaca ttgatggtga aagaaaaact gcccgatatt gtgctccgtg
     2821 gggttgcgtc gggagcaagg cctggtgtgg tgcttgagga gaaagggagg cagcagtttt
     2881 atttgcaggg agagagactc tcatccggtg atgctgtgat tgaacgtatt tttcctgagc
     2941 aggtgatgtt gcgttaccag gataacgtta tttacctcac actgaccgat gaagaaggtg
     3001 cccctgagcc agagaaaaaa gatgagcagc cagaaatatc ttctccgccg gaaacatctg
     3061 ctgttgctga tactttttcc cgtttgccac ccgcagttca gcaggcactg gccattgatc
     3121 ctcaaaaatt tttcgaatat atccgcttta cccccgtata taaggacggt gttgccggtt
     3181 acgctgtgat gcccgggggg gatcgctccc tttttgatat cagtggtctg aaagccggtg
     3241 atattgcggt tgccctgaac ggacaggatt ttacgcagcc gggagcaaca agcatcttta
     3301 tgcaacaaat ttattcaatg agtattgtca ggctgaccat tttacgaaac ggggagcgcc
     3361 acaacatatc tgttgcattg cgttgataat tccctgtctg tttttaattc aggagaacgg
     3421 cttaattatg ttccggtgtg aaattatgct gcgtgcccgg cgtgaagatg acaggcaaaa
     3481 aaaaataacc ggcattaaaa agaaaaaata tttactttct ctgatactga tggctgcatt
     3541 gtgtagtccg tcctcccggg ctgaagaaaa tacctttact gccagcttta aagatacaga
     3601 tttgaaatca ttcatcgaaa ccgtcggtgc taatcttaat aaaaccatcg tgatgggacc
     3661 agatgttcag ggaaaggtga gtattcgaac gataacacca ctcaatgaac gtcagtatta
     3721 tcagttgttc cttaatttgc tggaagccca ggggtatgcc gtcgtacccg tggagaataa
     3781 cgtacttaaa gttgttaaat ccggtgcggc gaaaacagaa ccattacccc tgaccggaga
     3841 aggccatgaa aattatgtcg gtgatgaaat ggttacccgg attgtgccgg tacgtaatgt
     3901 ttccgtacgt gagctggctc ctgtgttaca gcaaatgact gacctggacg gatccggcag
     3961 tattgtcaat tacgagccct ccaatgtgat tatgctgacc gggcgagcct cggtggtgaa
     4021 acgtctgact gaggtaatac aacgaattga tcgtgaaggg aaccgtaccg aggaggtgat
     4081 cccgctgggt aatgcgtcgg cttcagaaat cgcccgtgtg ctggaaagtc tgaccagaaa
     4141 cagcagtgaa aatcagcctg caacgctgaa atcacagatt gtcgctgatg agcgaacgaa
     4201 cagtgttatc gtcagtgcag atcccgccac acgggacaaa atacgccgtc tgatccgtcg
     4261 cctggactca gaaatggagc gcagtggcaa cagccaggtg ttctatctca aatacagcaa
     4321 agccgaagat ctggtcgatg tgctgaaaca ggtcagcggc acacttactt ccgccaaaga
     4381 agaagcagaa ggcactaccg gtagcaggcg tgatgttgtt tccatcgctg ccagcaaaca
     4441 cagtaatgcc ctgattgtca ccgcgccaca ggacatcatg cagtctttgc aaagcgtgat
     4501 tgaacaactg gatattcgtc gtgctcaggt gcatgtcgag gcgttaatcg cggaagtcgc
     4561 tgaaggcagt aatatcaact tcggtgtgca gtggatgtca aaagataccg ggtttatgca
     4621 gtttgccaac ggtacacaga tcccgattgg ttccctgagc atggcggttt ctcaggcaaa
     4681 gccgcagaaa ggctccacgg tgatcagcga aaacggtgcc accactataa accctgatac
     4741 ggaaggtgat ctttccagat tgtctcagtt actttctggt tttagtggca cggcagttgg
     4801 ggtggtgaag ggcgactggg ccgcgctggt ccaggcagtt aaaaacgact ccggtaccaa
     4861 tgtgctctcc atgccgagta tcaccacgct ggacaaccag gaagccttct tcatggtggg
     4921 gcaggatgtt ccggtattaa ccggatctgc ggtgggtgaa aataacagta atcctttcaa
     4981 cacggtagag aggaaaaaag tcggcatcat gctgaaagtc acgccgcaga ttaacgaagg
     5041 taacgcggta cagatggtga ttgagcagga agtctcgaag gtggaagggc agaccagcct
     5101 cgacgtcgtg tttggtgagc gcaaactgaa aaccaccgtg ctggctaacg atggcgagct
     5161 gattgtactt ggcggtctga tggacgacca ggcgggagaa agcgtggcga aagtgccgtt
     5221 gctgggcgat atcccgttga ttggcaacct gtttaagtcc acggcagata aaaaagaaaa
     5281 acgtaatctg atggtgttta tccgcccgac cattctgcgt gacggtatgg cggcagacgg
     5341 cgtgtcgcag cgcaaatata actatatgcg tgccgagcag atctatcgcg atgagcaagg
     5401 cttaagcctg atgccgcaca ccgcgcagcc ggtactgcca gcgcaaaacc aggccttacc
     5461 gccggaagta cgcgcgttcc ttaatgccgg gagaacgcgt taatggtgcc tgtagcacag
     5521 gaaaccaccg ctaacaccgt gcgtctgccc tacagtttca gccgccggtt tagcctggtg
     5581 gcatggtgcg aagcgtcgct ggaaatcctc catgttcatc cgttgtcgct ctctgttttg
     5641 caggagctgc agcgggggct ggacgcgccc tttacgctgc ggcaaatcga tgaggccgag
     5701 tttgaacagc gactgaatac ggtctggcag cgggactctt ccgaagctcg ccagttgatg
     5761 gaagatctcg gttccaccga ggacttcttt accctcgcag aagatctgcc ggaaacggaa
     5821 gatttgctgg aaagtgacga cgatgcgccg atcatcaaac tgatcaacgc catgctggca
     5881 gaggcgatta aagaaggcgc ttcggatatc cacatcgaga cgtttgaaaa gagtctggtg
     5941 atccgttttc gcgttgacgg cacattgcat gaaatgctgc gaccggggcg caaactggcc
     6001 tcgctgctgg tatcgcgtat caaggtgatg gcgcggctgg atattgccga aaagcgcgta
     6061 ccgcaggatg ggcgtattgc gctgctgctg ggcggccggg cgattgacgt gcgcgtctcc
     6121 accatgcctt ctgcctgggg cgagcgcgtg gtgctgcgac tgctggacaa aaaccaggcc
     6181 cgcctgacgc tggagcgtct gggtttgagt catgaactga ccgcgcagtt gcgccagctg
     6241 ttacacaaac cgcacggtat ctttctggtg acggggccga caggttccgg caaaagcacc
     6301 acgctgtatg ccggattgca ggagctgaac aaccattcgc gcaacattct cacggttgaa
     6361 gaccctatcg aatacatgat tgaagggatc ggtcagacgc aggtcaacac ccgcgtcggg
     6421 atgaccttcg cccgtggcct gcgcgcgatt ttgcgtcagg acccggatgt ggtgatggtc
     6481 ggtgaaatcc gcgataccga aaccgcagaa atcgctgtcc aggcttcact taccgggcac
     6541 ctggttcttt ccacgctgca taccaacacg gcggtggggg cgatcacgcg tctgcaggat
     6601 atgggcgtgg agcctttcct gctctcttcc agtctgacgg gcgtgatggc gcagcgactg
     6661 gttcgcacgc tgtgccccga ctgcagccag cccgcgcctg ccactgacga agaaaaacgc
     6721 ctgctgggga ttaccgatgc gcgtaccgtc acgctgtacc atccgcaggg ctgtcccgcc
     6781 tgtaatcaca aaggctttcg cggacgtact gccatccatg agctgctcgt ggtggacgcc
     6841 acattgcgtg atttgatcca ccgtcaggcc ggggaactgg agctggaacg ttatgtccgg
     6901 caacactctg cgggtatccg cagcaacggc attgagaaag tgctcgccgg agaaacctct
     6961 ctcgatgaag ttctgcgggt aaccctggag gcgtaatggc actgttttac tatcaggcgc
     7021 tggagcgtaa tggtcgcaaa accaaaggca tgattgaggc ggattccgcg cgtcatgccc
     7081 gtcaattgtt acgcggtaaa gacctcattc ccgtgcacat tgaagcccgg atgaatgcat
     7141 cggcaggggg aatgttgcag cgtcggcggc acgcacatcg tcgcgtggcg gcggcagatc
     7201 tggcgctgtt cactcgtcaa ctggcaacgc tggtgcaggc agcaatgccg ctggaaacct
     7261 gcttacaggc ggtcagtgag caaagcgaaa aactgcatgt aaaaagcctc ggaatggcgc
     7321 tgcgcagccg gattcaggaa ggttataccc tgtcggacag cctgcgcgaa catccccgcg
     7381 tctttgactc cctgttttgt tcgatggtcg ctgccggaga aaaatccgga catctcgatg
     7441 tggtgctcaa tcgcctggcg gattacaccg aacagcgcca gcgcctgaaa tcacgactgc
     7501 tgcaggccat gctctacccg ctggtgctgt tggtggtggc aacgggcgtg gtcactattt
     7561 tgctgacggc ggtggtgccg aaaattatcg aacagttcga tcacctcgga catgcgctgc
     7621 ccgcctccac ccgcaccctt atcgccatga gtgacgcgtt acaggccagc ggcgtttact
     7681 ggctggcggg attgctgggg cttctggtgc tggggcaacg gctactcaaa aatcctgcga
     7741 tgcatctgcg ctgggataaa accttgctgc gcctgcctgt gatagggcgg gtcgctcgcg
     7801 gactgaatac ggcgcgcttt tcccgcacgt taagcatcct caccgccagc agtgttccgc
     7861 ttctggaagg cattcagacc gctgccgccg tgtcggcaaa tcgctatgtc gaacaacaac
     7921 tgctgctggc ggcagagcgt gtccgcgaag ggagctgtct gcgcgccgcg ctggcggagt
     7981 tgcgcctgtt cccgccgatg atgttgtaca tgatcgcctc tggtgaacag agcggtgaac
     8041 tggaaaccat gcttgaacag gctgcagtca gccaggaacg ggaatttgat acccaggtgg
     8101 ggctggtttt ggggctgttt gaaccggcgc tggtggtgat gatggcaggc gtggtgctgt
     8161 ttatcgtcat cgccatcctc gaaccgatgc tgcaactgaa caatatggtt ggaatgtaat
     8221 ttacggagct atcacatgaa ttcgttatcc cgcacgcaaa aaccacgggc aggttttacc
     8281 ctgctggaag tgatggtggt gattgttatt cttggcgtcc tggcaagtct ggtggtgcct
     8341 aacctgttgg gcaacaaaga gaaagcggac aggcaaaaag ccatcagcga tatcgtggcg
     8401 ctggaaaacg cgctggacat gtaccgactg gataacgggc gttatccaac caccgagcag
     8461 ggactggagg cgctgatcca gcaaccggcc aatatggtgg actcccgcaa ctaccgtacc
     8521 ggtggataca tcaaacggct accaaaagat ccgtggggca atgattatca gtatctcagc
     8581 ccgggtgaaa aagggatgtt tgatgtttac accttagggg cggatggtca ggaaaacgga
     8641 gaaggggctg ccgcagatat cggtaactgg aatttacagg agcttcagta acaagtgcct
     8701 gaacgcggat tcactcttct ggaaatcata ctggtgattt tccttattgg tcttgccagt
     8761 gcgggggtag tacagacgct ggcaacctct tccgaaccgc ccgcgaaaaa agtagcgcag
     8821 gattttctga ttcggtttga acaactgaaa gataaagctg tgactgaagg gctggctgtc
     8881 ggtgtgttaa tcgagccctc cagctaccag tttatgcagc gtcgtcacgg gcagtggttg
     8941 cccgtttccg caccccggtt atcagcacag gttacggtgc cagaacaggt gcagatggtg
     9001 ttgcaacccg gaagtgatat ctggcagaag gagtatgtgc tggagctgca acgtcaccgt
     9061 ctgacgctgc atgacattga actggagctg caaaaagaga caaaaaagaa gaggccgcag
     9121 atccgttttt ttccctttga acctgccacg ccgtttacgc tgcgcttcta ctcggcagag
     9181 caaaacgaat gccaggcggt gaaactggcg cacgatggcg cgttatccct cagtcaatgt
     9241 gatgagagga tgccatgaag cgcggattta cgttgctgga agtgatgatt gcactggtga
     9301 ttttttcgct ggctgtcatg gcggtgttac agatcgccag cggcacgctg agtaatcagc
     9361 aggttcttga ggagaaaacg gtagcaggct gggtggcaga aaaccagacc gcactactct
     9421 atctgatgac ccacgaacag cgggcgatca ggcatcaggg cgagagtgat atggccggaa
     9481 gtcactggta ctggaaaacc ataccgctga aaacagacaa cgcactgtta caggcggtag
     9541 atattgaagt cagccgccac gaagactttt cgccggtgat tcagtcacga cgcgcctggt
     9601 tcagcgccgt tggaggccag cagtgaaaag gattctcgct ggttttacgt tactggaaat
     9661 gctgattgcg attgccattt ttgcctcact ggcattgatg acacagcagg tgacaaacgg
     9721 cgtcatacgt gcgaacagcg tcgtcgccgg acatgataaa aaactacaca tcgtgcaaca
     9781 ggcgatgagt tttctgaccc acgatctgac acaaatgatg ccgcgaccga taagaggcga
     9841 gcaggggcag cgggaaccgg cgttgctggc tggcgctggc gtgctggcgt ctgagagtga
     9901 aggaatgcgc tttgtgcgcg gcggcgtgat caatccgttg atgcgtctgc cgcgcagtaa
     9961 tctgcttacc gtgggttacc gtattcgcaa tggttatctt gaacgtcttg tgtggccgct
    10021 gacggacgca gcggatagtg tggagccggc aatacaaaaa cttttcccgg tggattcgct
    10081 ccgtttgcag ttccacgacg gcacgggctg gctggaggac tggtcgtcac agcagacgat
    10141 ccctgcagca gtacgtatga tcctgcattc accacaatgg ggcgatattg aacgaatctg
    10201 gttgttacat ggggctctgt catcatgaat ggctttcttc ccaggcgtgg tatggcgttg
    10261 ttgatggtgc tgctgttact ggcagtgatg gcatcagtgt gtaccggcct ctctcaccgc
    10321 atatcgcggc agacagacag aatgcgtagc ctgcaggatt atcaacaggc aacatggtat
    10381 gccgtcagcg cagaaagcct ggcactgagc atattaaatg aaaacttgaa aaatgaagaa
    10441 catactccgg tatattcgtg gagtatcggg tcacatttat ttccactgtc acaggggaaa
    10501 attgccgtca cgctacgcga tgcacagacc tgctttaacc tgaatgccct cgctcagccg
    10561 gcgaaggtgt caggggcgcc cggaagacaa caactcatta ccctgatttc ccgccttgat
    10621 gtgcctgtcc agcgggctgt gcagattact gaaagccttc aggtctttac tggcggaggt
    10681 cgtgacgggc aggcgcagca ggaaagtgaa tatcttaccc gaccagtgcc tttctggccg
    10741 gcaaagcagc cgctgacgga tatcagcgaa atacgtgttg tacagggaat ggatgccgcg
    10801 ctctatcaga aaattaaacc actggtctgc gcgctaccga tgagcaggca gcgaattaat
    10861 atcaattcgc ttgatgtatc acaaagtgtg attcttgagg cactgttcgg cccgggatta
    10921 agccccgttc aggcccgggc attactgcaa cagcgtccgg gaaaaggatg gcgcaatgtc
    10981 ggacagtttc ttgccagtcc ataccttaaa cgggttgatg agaatgtcag aaagcaggtc
    11041 ggggagtttc tgactgtgag cagtcattat ttctggttgc gttcagatat caccgtgaat
    11101 gagactgaac tgacgatgaa ctcgttaatt gcccgcacgg ggtcgcaaca cttttctgtt
    11161 ctctggcatc agacaggaga aagtgagtga gctccatgcc tgagattttt ttcccgcttt
    11221 gcgcctccga tcctgtccgc tggcaacgcc gtactgccga cgcggagcac ggcatctggc
    11281 ctgacattgc cgacgaacag cttcagcaat ggctgcaaac tgatgcgatt cgactctaca
    11341 ttcccggcga atggatcagc gtctggcagg ctgaactgcc tgatgtcccc cgctggcaga
    11401 taccgactat tctgcccgtt ttactggaag aagaactgaa ccaggatatc gacgaactgc
    11461 attttgcgcc gttgaaaatc gaccagcaac tggcaaccgt agcagtgatt caccagcatc
    11521 atatgcgcaa cattgcgcag tggttgcagg agaacggtat caccagcgct accgtcgcgc
    11581 cggactggat gtctattcct ggtggttata tggctggcga tgcgcaacgg gttatctgcc
    11641 gaatcgatga atgtcgggga tggagcgccg ggcttgcgct ggctccggcc atgttccgtg
    11701 cacagctcaa tgagcaggat ttaccgcttt cgctaaccgt ggtcggaatc gcgccggaaa
    11761 agctatctgc atgggctgat gcggatgccg aacgtctgac gatcacggct ctgccagcca
    11821 ttaacacatc cggagaacca gaaggtaact tgctgagcgg ccagtggcaa cctcgcgtca
    11881 gctaccgaaa acagtgggcg cgctggcggg tgatggttct gcctgtattg ctgattctgg
    11941 ttgcgctggc agtggaacgg ggcgtgacgt tatggagcgt cagcgaacag gtggcgcaaa
    12001 gtcgtgccca ggcagagaaa ctgttcttaa cgttgttccc ggagcagaag cggattgtga
    12061 atttgcgctc tcaggtggcg acggcgctga aaaaatatcg cccacaaact gatgataccg
    12121 atctactcgc tgagttgtca gcgctcgcca gcactctgaa atcagcggca ctttccgaca
    12181 tagagatgcg tggtttcacc tttgatcaaa accgccagac ccttcatctc cagttgcggg
    12241 ccgcaaactt tgccagtttc gacaaactgc gtagcgcact ggcggcagat tatgttgtgc
    12301 agcaggacgc gttacagaaa gagggtgatg cggtttccgg cggcgtaacg ttgcggagga
    12361 aataacatgt tacgcggtaa atttgttcat cgttttcagc aatggtgtga gcggcagctg
    12421 aacaacgctg aacagtggct ggtacaacac ctggaggggc gttcgtcacg tgaaaagggc
    12481 atgttactgg cagcagcggt gtttctgttt agcgccggat attacgtcct catctggcag
    12541 cctttgagcg aacggattga gcaacagaag acgatgctgc aacagctggt ggcgatgaac
    12601 gcgcgactga agagcgccgc gccggagatt attgcggcgc gaaaatccac cacaacaacg
    12661 cctgcacagg tatcgcagat catcagtgac agtgcgttat ctcgcgggat tgcgattaca
    12721 tggatggatg cacgaggaga gtatattcag gtccggaccg gagcggtagt ttttaacgat
    12781 ttgctggagt ggttaaatga gttgagagat gaatatgcac ttggtgttgc acaacttgat
    12841 gtgaaacagg cagaattatc aggaatggtt aatatacaga ggttagaact gacaaggcgt
    12901 tgataacagt aacatcacaa agtaccggaa cacacctggc agatgtcata ttttcttctt
    12961 gtctggcagt agttctggaa cagtacacag aacggtataa tcatgtgatt ttgatatatt
    13021 cactccatcg tcaaatgaag gttgaattat ggacgataaa caatagcaga ctcattaatg
    13081 aacggggcaa gaatcaagac gcctaaaaat atcaggcggc ttgagcgcca gttcaggaaa
    13141 atctgtgttg aaatcgtttt catcattgaa gtggctccat ccggagtact gaaaacaggg
    13201 gacactatta aacatattca gccaatcctt ttatgtcatc ggttaacagt gaaggtaaga
    13261 agacatactc gcgacgtaag gatgtggtgc atttactgac gtaccagagc agcaaggggc
    13321 tggagttccc gtatgtggcg gttatcaatg cttctttcgt tcactctggt gcagcggatg
    13381 agtctgaagt gattccggtg ctttatgtag cgttcacccg ggcaacccgt gaattactgg
    13441 tgacctgtta ccgtgaaaac agtatcagtc gtcaccttga ggattttgca taataccgga
    13501 ccatttcttt attctggttt tccactatat tttattatca ggtgtatttt atttatgatg
    13561 aatccattat tcagtgacat tcagatgcgt cttttttatt taaatcataa tccatattcc
    13621 tggcactggg atgttaaatt caaaccgtag gaagctgtat acatcggaaa taatgcatgt
    13681 catatcacca ttgctcataa tcagcccggg tatcatttga cgatggatgg agagagagta
    13741 cgaacggaat atcatatcgg aaatattcac ggactatttt cggttctaca acgacgatgg
    13801 gatgtgacac cggcaattat ccgcgcggtg gaataccttt cccgggtacc ggtaccacac
    13861 tgatgttctt tccgtaacgg ttaatcatga tttaatacag caacaccgaa tctgctgatg
    13921 acttctgaag tgaaactgaa aaccggtggt gacccccggt cattaccgga ttatgctgcc
    13981 ctgcgtgatg aaatcagcaa actgatccac ccggcacgcc cggatgtgga ctggcgttat
    14041 gtggaaacac tctgcctgcg tctgtatgaa cacaatggcg tggaattaca gacggcatcc
    14101 tggtacacca tcgcccggat gcatacaaca gggttaagtg gtctgaatga aggactggct
    14161 ctgattgtgg cgctgacccg gcatcactgg tcagtgatgt ggccgctgaa tacccacgcc
    14221 cggctggaaa ttattaccgg tctgttcaac cgtctgcaaa aaacgctccg ggccatgcca
    14281 ccggacgacc gtgacaacct gcccctgctg tatcagaccg aaacattcct gaaggcactg
    14341 agtgacacac tggcccggca cgaacttaaa cagagcagta aagtggcttt accggaagcc
    14401 atggtgaagg gatatatcac ccgcttggaa aatcagcctg tgcagggcga agcgtcgtct
    14461 ccggtaacgc ttcctgctca ggcactccgg tctgatgctc cggatgtgca ggaacatcag
    14521 cccctgccgc actcccgtct ggtgtatgtt gtttccgaaa caaaagcaga gcccacactc
    14581 acccttcaaa aatccccgcc cacgtttctg aaaccgtttg taactggtgt ctgtgcggca
    14641 ttgatggctg tcagcgtggc cattccggga tggcagtttc tgacacagcc atcaccggca
    14701 gagcagcaac tcagggcgct gctgacacca ccaccggatg tgctttcagc agaccagatg
    14761 acacagctca gcagaacacc ctcactgtta aatcatgcgt cagagtggat aaccctttcc
    14821 ggtcaacaga taacgcgact gacagaatta cccctggcat acaacctgca gcgcagtgcg
    14881 cagctactgc agcaactgat ggtacttttc cccgacaatc cccgggtaca ggaaatggtg
    14941 gataactggc agaagagcgt aaggagtcgt gcgctcccgg aagagacgat ggcgggctgg
    15001 aacgaaggca tgacccgctt acagcagctg gctgagcgcc tgaaccgtct ggatgaacag
    15061 cggggaagat acatgacagt cagtgaactt taatcgcaag tctttggcat gctgacaagc
    15121 ttccggcaga ctgtgccggt ggaagaacag attcggcagt tgaagctgct ccctgaagac
    15181 tctccgcaac gtcagcagca gattcagcag gctgagcagc acctgcgggc tcaagtctat
    15241 atgctggcgc aggaaaaaca tcgtgagtga tgtaaaagga actggcgagg tgggtaaaac
    15301 acagcggcac cgtaatcacc ggacacagtg gacagccacc gaaattacgt ttgtggaaca
    15361 acactatggt cagttgtctg cccgtaaaat cggagtgcgg cttggccggt ctgcccatgg
    15421 ggtggcccga attgttcagc ttttaggtct gtcccaaggc gtggcaccgc cctagtcgga
    15481 aatggaactg gtgttgatgc aggtgcacta cagtaaaggg attgataaaa taaaagcgtt
    15541 gctgccggat cgtacctggg aggccatttt tgcctaggcc ggaaaaatgg agctgactga
    15601 ggcccggttc tggcagtctg ttgaggaccg ggtattaagg aatatatatc ctgaaggcgg
    15661 tgttccgggg gggcgggaga ggtttcccca caggacgatc cctgctatca aacatcgggt
    15721 gaaggctttg gggcagaggt ccttcaaaag gaaaaaacgg atatcaagtg tgagagatga
    15781 tggtggagga tgaggttgca caaaagctgg aggttgctgg tctctggcgc agggctgtca
    15841 gtcgctggct tgatgtgatg cagcaccatg ggctgacggc tcagcaacgt gatcgaatac
    15901 gtcagcacag aaaatattgt ctttcctgcg taaagcccat cagcatccta gagaaactgg
    15961 atgtgacgga aattgctagg gctgcaaaga ccgtccagga gcggatgggg ctgagtcatc
    16021 cgtatggggt agctttcagg ttgaaagacg aaaaaataac agattttcgt catacaatga
    16081 gcactatact gcacaaagag ggttttgact ctgcgtggat tgaaaactca acttgcccat
    16141 gtggacaaga atgcgagccg tgagggcgca gtatctggat agaaggaaag agatgatgca
    16201 gtggtatgct tcgtttatat caaacttgag tgtgacaagt gtttaaaaca tagcaacttg
    16261 aggtatcgaa ttggcttgaa aatatctttt accagattga tttttaagcc acaatgcagc
    16321 aaagatcaca ttttcacaag atcgaagcgt agaggtgcag tcaccaatag ctggtatcat
    16381 aaacaagaat agagaggata ttgcatgggc agacctaatg aggtagggaa tgtcgataaa
    16441 agataaggct caacacggag gtttaaaccc taagacaaaa ctttgccatc atagtaaatg
    16501 gattactttt ctatccattg ttttttgcac cttagtaact ggtttttttt ggctcttcat
    16561 tttctattta attctacgcc caagttttta ctcttatatt ggatttgctg ataattttgt
    16621 gcttaaaaaa cctgatgtga tttctgatcc caagacttta cagaatattg gcaaaatggt
    16681 cactgatgga acccttttaa gtcttgacga tttttggtct ttccaaagta atttatatca
    16741 gacaattatt actgtgctca ttgcgctcaa tgggatagta gctgcatttt catttcttat
    16801 tattaaaaac tcttctacag caaaagcaaa agaagaagcg agagatattg ctgttatcga
    16861 agtaaatcaa tatgtagaaa gttcaaaatt tgaaaaagaa ataaaggcga ttttatacga
    16921 aaagatcgag cctgtacaat ttgatttact agctcaaata agttcaattg acgatattaa
    16981 acttagtcaa caagaacaca atcagtcatt tgaaaaactt gagcaagaac tggatggttt
    17041 aaaaaggaat cttaaattaa ttgctgaaag catttcttta aaagatacct cagatgaaga
    17101 tggcggcaac tttatcttag gaaggagaac ttaaatggcg tttatgacta agaaattaaa
    17161 aagcagtggt gtaataagcg gccaagacat tgatatgaca acggcttcag agcttattgt
    17221 ttttgctaag aaaaacaata ttgaaacaag ccctttagat gtttcaaggc ttaccagttt
    17281 gttaggtatt acattgcggc tagaaccgat gaaaggagaa gaatcgggtt ctctcaaaca
    17341 agggaaaagc ggcaattgga taatgacggt aaactcttta caccatcctc atcgccaacg
    17401 cttcacaatc gcccatgaac taggccattt tattcgccat acactcaata gaaatagttt
    17461 tacagacacg gttttcttca gaaatgaaga aacaaataaa atggaagctg aagcaaatag
    17521 attcgcagct gaattattaa tgccagagaa tgaatttagt aagtttatag aagaaaaatc
    17581 aaatcaagtg gctgatatag cagcacattt ccaagtgtct tcaatggctg tgagaattcg
    17641 tgctcaacaa ttaggcttca gtggacataa tttataaatc atgaaaaaat tcaattactt
    17701 tccaattatg aagactcggg acgctgagtt aaaagcgatg tcaaaactct cttcaggggt
    17761 atttgatcat attctaccta tttatgagct aacaaagtca agaaaagcca aaattgctcc
    17821 agatggagac attcatcgta gaatgctgac tatcaaagat attcagggtg aaagaccttt
    17881 tattttagat ttaacatcaa atgagaaata tattaacgat caaatccgac aactcgttga
    17941 tgaaaaggat
//
LOCUS       DAZKHK010000104        17880 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKHK010000104 DAZKHK010000000
VERSION     DAZKHK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.104"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            106..936
                     /locus_tag="ACHOS5_003682"
     CDS             106..936
                     /locus_tag="ACHOS5_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9778432.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            968..1903
                     /locus_tag="ACHOS5_003683"
     CDS             968..1903
                     /locus_tag="ACHOS5_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9778433.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQY"
     gene            1996..2238
                     /gene="yhdT"
                     /locus_tag="ACHOS5_003684"
     CDS             1996..2238
                     /gene="yhdT"
                     /locus_tag="ACHOS5_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP9778434.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2228..3679
                     /gene="panF"
                     /locus_tag="ACHOS5_003685"
     CDS             2228..3679
                     /gene="panF"
                     /locus_tag="ACHOS5_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP9778435.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFSISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3691..4572
                     /gene="prmA"
                     /locus_tag="ACHOS5_003686"
     CDS             3691..4572
                     /gene="prmA"
                     /locus_tag="ACHOS5_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9778436.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4901..5866
                     /gene="dusB"
                     /locus_tag="ACHOS5_003687"
     CDS             4901..5866
                     /gene="dusB"
                     /locus_tag="ACHOS5_003687"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP9778437.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5892..6188
                     /gene="fis"
                     /locus_tag="ACHOS5_003688"
     CDS             5892..6188
                     /gene="fis"
                     /locus_tag="ACHOS5_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP9778438.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6274..7158
                     /gene="yhdJ"
                     /locus_tag="ACHOS5_003689"
     CDS             6274..7158
                     /gene="yhdJ"
                     /locus_tag="ACHOS5_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9778439.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLITK"
     gene            7242..7421
                     /gene="yhdU"
                     /locus_tag="ACHOS5_003690"
     CDS             7242..7421
                     /gene="yhdU"
                     /locus_tag="ACHOS5_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP9778440.1"
                     /translation="MIRRYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7424..8086)
                     /gene="acrS"
                     /locus_tag="ACHOS5_003691"
     CDS             complement(7424..8086)
                     /gene="acrS"
                     /locus_tag="ACHOS5_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP9778441.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDYLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMACYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
     gene            8485..9642
                     /gene="acrE"
                     /locus_tag="ACHOS5_003692"
     CDS             8485..9642
                     /gene="acrE"
                     /locus_tag="ACHOS5_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP9778442.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADATVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMESGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            9654..12758
                     /gene="acrF"
                     /locus_tag="ACHOS5_003693"
     CDS             9654..12758
                     /gene="acrF"
                     /locus_tag="ACHOS5_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFP9778443.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIILVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYTLIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            13011..13232
                     /locus_tag="ACHOS5_003694"
     CDS             13011..13232
                     /locus_tag="ACHOS5_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778444.1"
                     /translation="MKRLIPVTLLIALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            13663..14688
                     /locus_tag="ACHOS5_003695"
     CDS             13663..14688
                     /locus_tag="ACHOS5_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9778445.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            14756..15937
                     /gene="yhdX"
                     /locus_tag="ACHOS5_003696"
     CDS             14756..15937
                     /gene="yhdX"
                     /locus_tag="ACHOS5_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9778446.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIIGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     FAAVLIIGLPLLAQWLFGAALLWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            15947..17050
                     /gene="yhdY"
                     /locus_tag="ACHOS5_003697"
     CDS             15947..17050
                     /gene="yhdY"
                     /locus_tag="ACHOS5_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9778447.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFIYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            17058..17816
                     /gene="yhdZ"
                     /locus_tag="ACHOS5_003698"
     CDS             17058..17816
                     /gene="yhdZ"
                     /locus_tag="ACHOS5_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9778448.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDDFFAHPKSERTRAFLSQVIH"
BASE COUNT         4347 a         4266 c         4527 g         4740 t
ORIGIN      
        1 gttgcaattt ttgacccatg attttctttt cgaattgtcg atatgattga aggataactt
       61 tcaaagaaaa atacaagggg ctatagtgtt acgcattaaa ggccaatgaa gagtgttaac
      121 gtgccgagaa aatcgtctaa cctactgaaa cgcagattaa aaattgcaga aattgttgcc
      181 gcccagggtg aaatcaaagt tgatgactta tctgcgcaac ttggtgtctc tggcgtgacg
      241 atccgcggcg atctcagtta tctggagcaa cagggatatt taaagcgatc atttggcggt
      301 gctattgcga caccactctc ctctgaacca gaagcgatac aaccgatcgc gccattgtct
      361 ctggcacaac aaatggaaat agcacgacat tgcgcacggt taattcgcga tcgtgacacg
      421 ctatttcttg gacatgggac aatctgccgt aagatcattc cattgctcag cgaagtgaaa
      481 aaactgcgtc tgatcaccaa cgatcccgaa catgcgctgc tggcaaatca gtttattgat
      541 ggcgaaataa tactggctgg cagtgaaatg ttgcgccccg acaccgcact ggccggaaaa
      601 cagcttgaac aggcacttca gcatttcacc attacacata gcattctgga aatcagccat
      661 ctggatgctg atggtacttt aaatatcaac gttcccagac tcgccgctgc ctggcagctc
      721 tgtttcgatc atgcccagaa taagacggtt atcgttgccg ccgatcctgc gccatcaacg
      781 gctgctatcg ggcatttgca tcaggcagaa aatgtgatcg tcagccgcag cattaacgaa
      841 caatatcagc aacaattgca gtcggctgat ttcgttatcc tttataccag taatgagtgc
      901 tttacctggt ccaatcgcga aaggcttcag gaatgagagg ggttttgtga caataactat
      961 ctgcgctatg ggcgaattgc tggccgagtt tttgtcccgc aacccacatc aaaaattcac
     1021 tcagcctggg gagtttatcg ggccatttcc cagcggtgcg ccagcaattt ttgctgctca
     1081 ggtggcaaaa ctgtcccatc gggccatctt ctttggatgt gttggtaatg atgattttgc
     1141 ccgactcatt atagagcgtc tccgtcatga aggtgtcatt accgatggga tccatgttat
     1201 gaacaatgcc gtcacaggta cggcgttcgt gagttatcaa aatccccagc tgcgggattt
     1261 cgtctttaat atccctaaca gcgcctgcgg tttgtttact gccgagcaca ttgataagga
     1321 tctgcttaaa cagtgtaacc atctgcatat tgtgggctca tcgttgttct catttcgcat
     1381 gatcgatgtc atgcgtaaag caataacgac gatcaaatcg gctggcggca ccgtttcttt
     1441 cgatcccaat attcgcaaag agatgctgag cattcctgaa atggcgcagg ctctcgatta
     1501 tttgattgaa tatacggata tttttatccc cagcgaaagc gaactccctt tcttcgcgcg
     1561 tcacaaaaat ctgtcagagg aacagattgt tagcgatctt ctccacggcg gcgtaaaaca
     1621 tgtggcgata aaacgcgccc agcgtggggc cagctattac aagcttaaaa acggtacatt
     1681 acacgcccag catgttgcag gtcacgatat cgaaattatc gatccaacgg gtgcaggcga
     1741 ctgctttggc gcaacgttta tcactctttt cttatccggt ttcccggcac acaaggcgct
     1801 gcaatatgca aatgccagcg gcgcgctcgc cgtaatgcgg caaggtccga tggaagggat
     1861 atcctcactg gcagacattg aagacttttt gcagcagtac tgaacggcaa ttttctgtca
     1921 gatcattttc cctgcctgaa gacttgccgt agaattgccc gtttctcgta tccccccatg
     1981 aggaggaccc cttccgtgga cactcgtttt gttcaggccc ataaagaggc gcgctgggcg
     2041 ctggggctga cccttttgta tctggcggta tggttagtag ccgcttactt acctggcgtt
     2101 gcccccggtt ttaccggctt tccgcgctgg tttgagatgg cctgcatcct gacgccgctg
     2161 ctgtttattg gactgtgctg ggcgatggtg aaatttatct atcgcgatat cccactggag
     2221 gatgacgatg cagcttgaag taattctacc gctggtcgcc tatctggtgg tggtgttcgg
     2281 tatctctgtt tatgcgatgc gtaaacggag cactggcacc ttccttaatg agtatttcct
     2341 cggcagccgc tctatgggcg gtattgtgct ggcgatgacg ctcaccgcga cctatatcag
     2401 tgccagttcg tttatcggcg ggcctggagc tgcttataaa tacggcctgg gctgggtatt
     2461 gctagcgatg attcagcttc ctgcagtctg gctttcgctc ggtattctcg gcaagaagtt
     2521 cgcaattctt gcgcgccgct acaatgcggt gacgctgaac gatatgctgt ttgcccgcta
     2581 ccagagtcgt cttctggtat ggctggcgag tttgagtttg ctggttgcgt tcgttggtgc
     2641 gatgaccgtg cagtttatcg gcggtgcgcg cctgctggaa accgcggcgg gtattcctta
     2701 tgaaaccggg ctgctgattt tcagtatcag cattgcgttg tataccgcct ttggcggctt
     2761 tcgcgccagc gtgctgaacg acaccatgca agggctggtg atgctgattg gtaccgttgt
     2821 gctgcttatt ggcgtggtac atgccgcagg tggtttaagt aatgcggtag acacgctgca
     2881 aaccatcgat ccgcaactgg ttacgccaca aggcgctgac gatattctgt cgcctgcctt
     2941 tatgacgtcg ttctgggtac tggtgtgctt tggcgttata ggcctgccgc atactgcggt
     3001 gcgctgtatc tcttataaag acagcaaagc cgtacatcgg ggaatcatta tcggtacgat
     3061 tgtagtcgca atcctgatgt tcggtatgca tctggccgga gcgttaggtc gggcggtgat
     3121 ccccgatctc accgtaccgg atctggtgat cccaacgtta atggtaaaag tgctgccacc
     3181 gtttgctgcc gggatcttcc tggctgcgcc tatggctgcg atcatgtcga caattaacgc
     3241 ccaactgctg caaagttccg ctacgatcat taaagatctc tatctgaata tccgtccgga
     3301 tcaaatgcaa aacgagacgc gtctgaagcg gatgtcggcg gtgatcacgt tggttctcgg
     3361 tgcgttgctg ctgcttgccg cctggaagcc gccagaaatg atcatctggc tgaatttgct
     3421 ggccttcggt gggctggaag ccgttttcct gtggccgctg gtgctgggtc tttactggga
     3481 acgcgccaac gccaaaggcg cgctaagtgc gatgatcgtt ggcggcgtgc tgtatgccgt
     3541 actcgcgacg ctgaatattc agtacctggg cttccaccct atcgtgccct cgttactact
     3601 aagtttgctg gctttcctgg tcggaaaccg tttcggtaca tccgtcccgc aagctaccgt
     3661 tttgactact gataaataaa gagttttgcc atgccttgga tccaactgaa actgaacact
     3721 accggcgcga acgcggaaga tcttagcgat gcgctgatgg aagcgggtgc cgtttctatc
     3781 acttttcagg atacccacga tacgccagta tttgagccgc tgccgggcga aacgcgcctg
     3841 tggggcgaca ccgatgtgat tggtctgttc gacgctgaaa ccgatatgaa cgacgtggtg
     3901 gcgattctgg aaaaccatcc gctgctcggc gcgggcttcg cgcataaaat cgaacaactg
     3961 gaagataaag actgggagcg cgaatggatg gataatttcc acccgatgcg ctttggtgaa
     4021 cgactgtgga tctgcccaag ctggcgtgat gtgccggacg aaaacgccgt caacgtgatg
     4081 ttagacccag ggctggcgtt tggtacgggt acccatccaa ctacctctct gtgcctgcaa
     4141 tggctcgaca gcctcgattt aaccggtaaa acagtcatcg actttggctg cggttccggc
     4201 attctggcta tcgcggcgct gaaactgggt gcagcaaaag ccattggtat tgatattgat
     4261 ccgcaggcga ttcaggccag ccgcgacaac gctgaacgta atggcgtttc tgatcgtctg
     4321 gaactctact taccgaaaga tcagccagaa gaaatgaaag ccgacgtggt ggtcgctaac
     4381 atccttgcag gtccattacg tgaactggca ccgttaatca gcgtcctgcc agtttcaggc
     4441 ggtttgctgg gcctttccgg tattctggca agccaggcag agagcgtttg cgaagcttat
     4501 gccgacagct tcgctctgga cccggtcgtg gaaaaagaag agtggtgccg cattaccggt
     4561 cgtaagaatt aaccttcgca tcgccgtatg gcgacgcaga gcgaagtgcg agcgatgtca
     4621 caaaaggcac gttaatttgc cgattattta cgcaaatttg cgtgccaaaa ttttcattca
     4681 taaagaaaaa ttgagaactt actcaaattt ctttgagtgt aaattttagt cactattttc
     4741 taatatgatg atttttatga gtaattatcg cgctacgctc attttaaatg caattctttg
     4801 atccatctca gaggattggt caaagtttgg cctttcatct cgtgcaaaaa atgcgtaata
     4861 tacgccgcct tgcagtcaca gtatggtcat ttcttaactc atgcgcatcg gacaatatca
     4921 gctcagaaat cgcctgatcg cagcgcccat ggctggcatt acagacagac cttttcggac
     4981 gttgtgctac gagatgggag ccggattgac agtatccgag atgatgtctt ctaacccaca
     5041 ggtttgggaa agcgacaaat ctcgtttacg gatggtgcac attgatgaac ccggtattcg
     5101 caccgtgcaa attgctggta gcgatccgaa agaaatggca gatgcagcac gtattaacgt
     5161 ggaaagcggt gcccagatta ttgatatcaa tatgggttgc ccggctaaaa aagtgaatcg
     5221 caagctcgca ggttcagccc tcttgcagta cccggatgtc gttaaatcga tccttaccga
     5281 ggtcgtcaat gcagtggacg ttcctgttac cctgaaaatt cgcacaggct gggcaccgga
     5341 acaccgtaac tgcgaagaga ttgcccaact ggctgaagac tgtggcattc aggctctgac
     5401 cattcatggc cgtacacgcg cctgtttgtt caatggagaa gctgagtacg acagtattcg
     5461 ggcagttaag cagaaagttt ccattccggt tatcgcgaat ggtgacatta ctgacccgct
     5521 taaagccaga gctgtgctcg actatacagg ggcggatgcc ctgatgatag gccgcgcagc
     5581 tcagggaaga ccctggatct ttcgggaaat ccagcattat ctggacactg gggagttgct
     5641 gcccccgctg cctttggcag aggttaagcg cttgctttgc gcgcacgttc gggaactgca
     5701 tgacttttat ggtccggcaa aagggtaccg aattgcacgt aaacacgttt cctggtatct
     5761 ccaggaacac gctccaaatg accagtttcg gcgcacattc aacgccattg aggatgccag
     5821 cgaacagctg gaggcgttgg aggcatactt cgaaaatttt gcgtaaacag aaataaagag
     5881 ctgacagaac tatgttcgaa caacgcgtaa attctgacgt actgaccgtt tctaccgtta
     5941 actctcagga tcaggtaacc caaaaacccc tgcgtgactc ggttaaacag gcactgaaga
     6001 actattttgc tcaactgaat ggtcaggatg tgaatgacct ctatgagctg gtactggctg
     6061 aagtagaaca gcccctgttg gacatggtga tgcaatacac ccgtggtaac cagacccgtg
     6121 ctgctctgat gatgggcatc aaccgtggta cgctgcgtaa aaaattgaaa aaatacggca
     6181 tgaactaatt caggttagct aaatgcttga ttaaaaaggc gctactcggc atggggaagc
     6241 gcctttttta taggtgtcac aaagggagtg accatgagaa caggatgtga accgacccga
     6301 tttggtaatg aagcaaagac catcatccag ggtgatgcat taaccgaact taaaaagctc
     6361 cccgccgaaa gtgtcgatct gatctttgcc gacccaccgt ataacatcgg taaaaatttt
     6421 gatggtctga tcgaagcctg gaaagaagat ctgtttatcg actggctgtt tgaagtgatt
     6481 gcagagtgcc atcgcgttct gaaaaagcag ggcagcatgt acatcatgaa tagcacggaa
     6541 aacatgccct ttatcgatct ccagtgccgc aagcttttta ccatcaaaag tcgcatcgtc
     6601 tggtcatatg acagttctgg agtgcaggcg aaaaaacact acggctccat gtacgaaccc
     6661 atcctgatga tggtgaaaga cgcaaagaac tacacgttca acggtgatgc tattctggta
     6721 gaagccaaaa ccggatcgca gcgcgcgttg atcaattatc gcaaaaatcc tccacagcct
     6781 tacaatcatc aaaaagtacc gggtaacgtc tgggattttc cgcgcgtgcg ttatttgatg
     6841 gatgaatatg aaaaccaccc gacgcaaaaa ccggaagcct tgctgaaacg cattattctc
     6901 gcctcttcca acccaggcga tatcgttctc gacccgtttg ccggtagctt tactaccggt
     6961 gccgtagccg tcgccagcgg gcgaaaattc attggtattg agatcaacag cgagtacatc
     7021 aaaatggggc ttcgacggct ggatgtcgcg tcgcattact ctgcggaaga actggcgaaa
     7081 gtgaaaaaaa gaaagaccgg caacctgtca aaacgaagcc ggttaagcga agttgacccc
     7141 gatctcatta caaagtaaag gaagtgtaag cctggttttc agattattca tttcgtgtat
     7201 atttcctgcc agacttggtt aaacatgcac aggctcaggt aatgattcgc aggtattggt
     7261 ggctcgtcgt tttcgctgtc ttcgtttttc tgtttgatac tttactgatg cagtggattg
     7321 aactgctggc aacagaaaca gacaaatgcc gcaatatgaa ctcagttaat ccactaaaac
     7381 tggttaactg tgacgaactg aattttcagg acagaatgtg aatttatatg acacttaatt
     7441 cattcgtttt atgaattaat ttcgttatgt tttcatctgg catgaacatt cttaatacgt
     7501 tatcgaccag tgcgggtgct tgtttataaa ggtcataaca cgccatattc attaaccagt
     7561 tttgaacaat tccgctgaag gcaccatcaa taataatcat cacaacatct aaatcgagat
     7621 tatttgctac acaaccttgt tgctgacacg cctgcaatac ttcgcggaga gtctgcggat
     7681 taaagcccat cttttcgcgt atcactccct cggccagcat ctcatcatta aattcacatt
     7741 tgtgatataa gattttcagc aacgcctgct ggcggggaat tttggcaata tattgcaaac
     7801 cgacaatcaa tttttcacgc aattgctgaa acgggtcatg ctctaaccca gcggtcaagt
     7861 aatcctggat taactcccgc aatgaaggct gttgcaacca catctcatta aacagttgag
     7921 tcttgttttc gaagtgccag tagatagcgc cacgcgtaac gttagcagcg tcggcaatgt
     7981 cgttgagcgt cgtcttgctt acgccatgct gcgcaaactg ggcgatggca gtttcaatca
     8041 gttcttgccg ggtcttcagg gcttcagctt tggttctttt tgccatgatt aattattcag
     8101 gaaataaata tattcaacac aggatgagaa aatagcgaag gttaatctat cacctaatgt
     8161 gtatttatac gagaggctaa tattgcgttg ctataaatcg tcaaataaat gatatgtatt
     8221 atttacctaa aatacattca ccctacctat atatatttca atttacgagg ttttaattct
     8281 acttctttaa atccgcgtca aaataaaaca ataggatatt aatctttttt tgtgtttatg
     8341 tgccttgagt cgccagtatt cataactatt ccttacatcg acgaatgata atttgtagga
     8401 tagcgaactg tatttttctt tctgcgagtt aacgcattgc ctttttgggc aaataacgcg
     8461 cttttggttt tttgaggaat agtaatgacg aaacatgcca ggtttttcct cctgccctcc
     8521 tttattctga tctccgcggc tttaatcgcc ggttgtaacg ataagggaga agagaaagct
     8581 cacgtcggtg aaccgcaggt taccgttcat attgtgaaaa cggccccgtt agaagttaag
     8641 actgaattac caggccgcac caatgcttac cgtatagccg aagttcgccc acaggttagc
     8701 gggatcgtac tgaatcgcaa tttcactgaa ggcagcgatg tgcaagcagg ccagtccctg
     8761 taccagatcg atcccgcgac ctatcaggca agttatgaca gcgcgaaagg cgaactagcg
     8821 aaaagtgaag ccgccgccgc catcgcgcat ttgacggtaa aacgttacgt tccgctcgtg
     8881 ggtacgaaat acatcagtca gcaggagtac gaccaagcca ttgctgatgc tcgccaggcc
     8941 gatgccaccg tgattgccgc aaaagccaca gttgaaagcg ctcgcatcaa tctcgcttat
     9001 accaaagtga ctgcgcctat tagcggacgt atcggcaaat cgactgtgac cgaaggcgct
     9061 cttgtcacta atgggcaaac gactgaactg gcgactgtcc agcagctcga tcctatctac
     9121 gttgatgtga cccaatccag caacgatttt atgaggctga agcaatccgt agagcaagga
     9181 aatttgcata aggaaaacgc caccagcaac gtagagttgg tcatggagag cggtcaaacc
     9241 tatcccctga aaggtacgct gcaattctcc gatgtgaccg ttgatgaaag caccggctcc
     9301 ataaccctac gtgctgtctt ccctaacccg caacatacgc ttttgccggg tatgtttgtg
     9361 cgcgcacgga ttgatgaagg cgtccaacct gacgccattc ttatcccgca acaaggcgtt
     9421 agccgcacac cacgtggtga tgcaaccgtg ctgattgtta acaataaaag tcaggttgaa
     9481 gcgcgccctg tcgttgccag ccaggcgatt ggcgataaat ggttgattag cgaagggctg
     9541 aaatctggcg atcaagtcat tgtcagcggc ctacaaaaag cgcgtccggg agagcaggtt
     9601 aaagccacta ccgatacccc cgcagatact gcatcgaagt aaggcaatct gacatggcaa
     9661 acttttttat tcgacgaccg atatttgcat gggtgctggc cattattctg atgatggcgg
     9721 gcgcactggc gatcctacaa ttgcccgtcg cccagtatcc aaccattgcg ccgcctgcgg
     9781 tttctgtttc ggcaaactat ccgggtgctg atgcgcagac cgtgcaggat acagtgacgc
     9841 aggttatcga acagaatatg aacggtatcg ataacctgat gtatatgtcc tccaccagcg
     9901 attccgccgg tagcgtgaca attaccctta ccttccagtc cgggaccgat cctgatatcg
     9961 cgcaagtaca ggtgcagaac aaactccagc tcgccacgcc gttgctgccg caggaggttc
    10021 agcagcaggg gatcagtgtt gaaaagtcca gtagcagcta tttgatggtg gcgggctttg
    10081 tctctgataa ccctggcacc acacaggacg atatctcgga ctatgtggcc tctaacgtta
    10141 aagatacgct tagccgtctg aatggcgtcg gtgacgtaca gcttttcggc gcacagtatg
    10201 cgatgcgtat ctggctggac gccgatctgc taaacaaata taaactgaca ccggttgatg
    10261 tgattaacca gttgaaggta cagaacgatc agatcgctgc cggacagttg ggcggaacgc
    10321 cagcgttacc agggcaacag ttgaacgcct cgattattgc tcagacgcgg ttaaaaaatc
    10381 cggaagaatt cggcaaagtg accctgcgcg taaacagtga cggctcggtg gtgcgtctga
    10441 aagatgtcgc acgggttgaa cttggcggtg aaaactataa cgttatcgct cgcatcaacg
    10501 gaaaaccggc ggcgggcctg gggattaagc tggcaaccgg cgcgaatgct ctcgataccg
    10561 cgaaagccat taaggcaaaa ctggcggaat tacagccatt cttcccgcag ggaatgaagg
    10621 ttctttaccc ttatgacacc acgcctttcg tccagctttc tatccaggaa gtggtgaaaa
    10681 cgctgttcga agccattatc ctggtattcc tggtgatgta tctgttcttg cagaacatgc
    10741 gggcaacatt gatacccacc attgctgtac ctgtcgtgtt gttagggaca tttgccatac
    10801 tcgccgcttt tggttactcc atcaatacgc taacgatgtt tgggatggta cttgccatcg
    10861 gacttcttgt tgatgatgcg atagtcgttg tggagaacgt cgagcgcgta atgatggagg
    10921 ataaactccc ccccagagaa gcgacggaaa aatcgatgtc gcaaatccag ggcgcgctgg
    10981 taggtatcgc gatggtactg tcggcggtat ttattccgat ggcattcttc ggtggctcaa
    11041 caggggcaat ttatcgccag ttctctatca ctattgtttc agcaatggcg ctttcagtac
    11101 tggttgcgct gattctcacc cccgcgttat gtgcaacatt gctgaaacca gtctctgccg
    11161 agcatcacga aaataaggga ggattcttcg gctggtttaa caccactttc gatcatagcg
    11221 ttaatcacta caccaatagc gttggcaaaa tcctcggttc cacagggcga tatttactga
    11281 tctatacgct gattgttgca ggaatggtgg tgttgttttt acgtcttccg tcttccttct
    11341 tacctgaaga ggatcagggt gtctttctga ccatgattca gttacccgct ggcgcgacgc
    11401 aagagcggac gcaaaaagtg ttggatcaag tgacggatta ctatctgaag aacgagaaag
    11461 cgaacgttga aagtgtcttt acagttaacg gctttagctt cagcggccag gcacaaaacg
    11521 ccggtatggc cttcgtcagt ctgaaaccgt gggaagagcg tagtggtgac gaaaacagtg
    11581 cggaagcagt aatccatcgt gccaaaatgg aattgggcaa gatccgcgac ggttttgtca
    11641 ttccatttaa tatgccagcc attgttgaac tgggcacggc aacgggtttc gactttgagt
    11701 taattgatca ggctgggctg ggtcacgatg ccctgaccca ggcccgtaac cagttgcttg
    11761 gcatggcggc gcaacatcct gccagcttag tcagcgtgcg ccctaatggc ctggaagaca
    11821 ccgcgcagtt taaactggaa gttgaccagg aaaaggcgca ggcattaggt gtttcacttt
    11881 ctgacatcaa tcagaccatt tcaacggcgt tgggtgggac ttacgttaac gacttcatcg
    11941 accgtggtcg cgtgaaaaag gtgtatgttc aggcggatgc caaattccgt atgctgccag
    12001 aagatgtcga taaactttat gtccgcagcg ccaacggcga aatggtgcca ttctctgcct
    12061 ttaccacttc acattgggtg tatggctctc cgcgactgga acgctacaac ggtctgccgt
    12121 caatggagat tcagggggaa gccgcgccag gaaccagttc cggcgatgct atggcgttga
    12181 tggaaaacct tgcgtcaaaa ttaccggcgg gcattggtta tgactggacg ggtatgtcat
    12241 atcaggaacg tttatcggga aaccaggctc ccgctctggt agcaatttcc tttgtggttg
    12301 ttttcctgtg ccttgctgca ctctatgaaa gctggtcaat tcctgtctcg gttatgttgg
    12361 tcgtgccgtt agggattgtc ggcgtgctac tggcggcaac actctttaat caaaaaaatg
    12421 acgtctactt tatggtgggc ttgctaacga caattggctt gtcggccaaa aacgctattt
    12481 tgattgttga gttcgctaaa gatctcatgg agaaagaggg taaaggtgtt gttgaagcga
    12541 cactaatggc agtacgtatg cgtctgcgtc ctatcctgat gacctctctc gcctttattc
    12601 tcggcgtatt accgctggct atcagtaacg gggccggcag tggcgcgcaa aacgcagtcg
    12661 gtatcggggt aatgggcgga atggtctctg caacgttgct ggcaatcttc ttcgtgccag
    12721 tgttctttgt ggtgatccgt cgatgcttta aaggataaat cagaaacata aaggcgcttt
    12781 caggcgcctt ttttattttc agtgaacccc ataaaaatta ataagatatt cttcttctca
    12841 ctctttaaaa actttctata gttcccgctc ccttcactat ttttacaatt cacataaatt
    12901 gtcattattc attccgcaag atttataacc tccagtgata aaatggcatt cagctcgtta
    12961 ataagagagt taacttatta agcgttagcg tttattactg aggtaacacc atgaaaagat
    13021 taattcctgt gacattgctc atcgcattgc tggcgggctg tgctcacgat tcgccctgcg
    13081 ttccggttta tgacgatcag ggtcgtctgg ttcacaccaa tacctgtatg aaaggcacga
    13141 ctcaggataa ctgggaaacg gctggggcta ttgccggcgg ggcagcagct gttgctggcc
    13201 tgacgatggg gatcatcgct ctgtcgaaat aactaaacag ggcgcggagc tccgcgctct
    13261 ttttaacgat acagtatctt tccataagat aaaatattgc tatattctgc tgggtaattc
    13321 cctgctgatt tagcatgtta tatcacgtta tttcgctgct ttcgaaataa cgcatcaatt
    13381 taactttcac acatcccacc agaaatatac tcacgctaat aatcattatt ctgcttagta
    13441 aaaaacgcgt aaatattttg cgccaaaatg tggcgcatgt ttcattttcg caccattgcg
    13501 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
    13561 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
    13621 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
    13681 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
    13741 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
    13801 gggttctctt atgccgatgc tgacggtaag ttttcaggta ttgatgtaga tgtttgtcgt
    13861 ggcgttgccg ctgctgtatt cggtgacgat acgaaagtga aatatacccc gctcactgca
    13921 aaagaacgct tcaccgcttt acagtcaggg gaggtggatt tgctctcccg taatacgacc
    13981 tggacttcat ctcgcgatgc cgggatggga atggcattta ctggcgtcac ttattacgac
    14041 ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga actggatggc
    14101 gctaccgtct gtattcaggc ggggactgat accgaactca acgtcgccga ttacttcaag
    14161 gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc agcgaaggca
    14221 ctggaatctg gtcgctgcga tacgctagcc tcggatcaat cacaactgta tgccctgcgc
    14281 atcaaattaa gcaacccagc tgaatggatt gtcttaccgg aagttatctc taaagaaccg
    14341 cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg ctggacgctt
    14401 ttcgccatgc tgaatgctga agagatgggc atcaattccc agaacgtcga tgaaaaagcg
    14461 gctaatccag caacgcctga tatggcacat ctgctgggta aagaaggcga ttacggcaag
    14521 gatctgaagc tggataataa atgggccttt aacatcatca aacaggtggg taactactcg
    14581 gaaattttcg agcgtaacgt aggttcagaa agcccgctga aaattaaacg tgggcaaaat
    14641 aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt cggtgtgagg
    14701 taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg tttctatgtc
    14761 tcatcgccgc tcaaccgtta aaggctcact ctcttttgcc aaccctacgg ttcgcgcctg
    14821 gttattccag atccttgccg ttgttgctgt tgtcggcatt attggttggt tatttcacaa
    14881 cactgtaacg aatctcagta atcgcggcat aacatcagga tttgcttttc ttgaccgcgg
    14941 ggccggtttt ggcatcgttc agcatttgat tgattatcag caaggcgaca cttacggacg
    15001 cgtttttatt gtcggcctgc tcaatacgct actggtttct gcattgtgta ttgtgttcgc
    15061 ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc tgctacgaaa
    15121 gctttccaca atttatattg agatcttccg taatattccg ccattgctgc aaatcttctt
    15181 ctggtacttt gccgtattgc gcaatttgcc cggaccacga caggcagtga gcgcgtttga
    15241 tctcgccttt ttgagcaatc gtgggcttta tattccgtcg ccgcagctgg gagacggatt
    15301 tattgcgttt atcctcgctg ttgttatggc tatagtcctt tcagttgggc tattccgctt
    15361 taataaaaca taccagataa agaccggaca actgcgccgc acctggccat tcgccgctgt
    15421 gttgatcatt ggtttgcctt tactggcgca atggcttttt ggcgcagcac tactctggga
    15481 tgtcccagcc cttcgaggct ttaatttccg cggcgggatg gttttaattc ctgaactggc
    15541 agccttaacg ctggcgcttt cggtttatac ttctgcattt atcgccgaaa ttatccgcgc
    15601 agggatccag gcagtgcctt atggtcaaca tgaagcagct cggtcactgg gattacccaa
    15661 cccggtcacg ctacgccagg tcattattcc ccaggcattg cgggtgatta ttccaccgtt
    15721 aaccagccag tatctcaaca tcgtcaagaa ctcctctctt gccgccgcta ttggctatcc
    15781 cgatatggtt tcgctgtttg ctggcaccgt gctgaaccaa acggggcaag ccatcgagac
    15841 gatagccatg accatgtcgg tctatctgat tatcagcctg actatctcgc tgctgatgaa
    15901 tatctataac cgccgcatcg cgatcgttga acgctaagga gtcatgatga caaaagtatt
    15961 gctgtctcat cccccgcgcc cggcgagcca taactcaagc cgcgcgatgg tgtgggtgcg
    16021 aaaaaatctg ttctccagct ggagcaatag cctgctgact attggctgca tatggttgat
    16081 gtgggaactg atcccaccat tgctgaactg ggcatttttg caggctaact gggttggctc
    16141 aacgcgtgcc gactgcacaa aagccggtgc ctgttgggtc ttcatccacg aacgatttgg
    16201 tcagtttata tatggacttt acccacacga ccaacgctgg cgaattaacc tggcattact
    16261 gattggcctt gtgtctatcg caccaatgtt ctggaaaata cttccgcatc gcggtcgcta
    16321 tattgcggtc tgggcggtga tttacccact ggttgtctgg tggctgatgt atggcgggtt
    16381 tcttggtctt gatcgggttg aaacccggca atggggcggg ttgacgctaa ctttaattat
    16441 tgcatcagtt gggattgcgg gggcgctacc gtgggggatc ttactggcgt taggtcgccg
    16501 ctcccatatg ccgattgtgc gtatcttatc ggtcattttt atcgagttct ggcgcggcgt
    16561 accgctgatt accgtattgt ttatgtcttc ggtcatgctg ccgttgttta tggcagaagg
    16621 caccagtatc gacaaactaa tccgcgcgct ggttggcgtg atcctgtttc agtcagcata
    16681 tgttgcggaa gtcgtgcgag gcggattaca ggcgctgcct aaagggcaat atgaagcggc
    16741 agagtcgctg gcattgggtt actggaaaac tcaggggctt gttattctgc cacaggcgtt
    16801 gaagctggta attcctgggc tggtaaatac catcatcgca ctcttcaaag ataccagcct
    16861 ggtgatcatc atcgggttgt tcgatctttt cagtagcgtt cagcaggcaa ccgttgatcc
    16921 cgcctggttg ggtatgtcga cggaagggta tgttttcgcc gcgctgatct actggatctt
    16981 ctgtttcagc atgtcacgtt atagccaaca tctggaaaaa cgttttaaca ccgggcgtac
    17041 accgcattga ggacactatg agccaaattt tactgcaacc tgctaacgcg atgattacgc
    17101 tggaaaacgt caataaatgg tatggacaat tccatgtttt gaaaaatata aatttaaccg
    17161 tgcaaccggg cgaacggatc gttctatgtg gcccttcagg ttccggtaaa tcgacaacca
    17221 ttcgttgtat taatcatctg gaagaacatc aacagggacg gatcgtggta gatggcatcg
    17281 aacttaatga agatatccgc aatattgagc gcgtcaggca ggaagtggga atggtctttc
    17341 agcatttcaa tctcttccct catctgaccg tgttacagaa ctgtaccctg gcaccgattt
    17401 gggtacgcaa gatgccaaag aaagaggctg aagctctggc gatgcattac cttgagcggg
    17461 tgagaattgc cgaacatgcc cagaagtttc ccggacagat ttctggtggt cagcagcaac
    17521 gtgttgccat agcacgttcg ctgtgtatga agccgaaaat tatgttgttt gatgagccaa
    17581 cgtcggcgct cgatcctgag atggtgaaag aggtgctgga tacgatgatt gggctggcgc
    17641 agtcgggtat gacaatgttg tgtgtaacac atgagatggg gtttgcacga accgtcgctg
    17701 accgggtgat ttttatggat cgtggggaaa tagtggagca agctgcacct gatgactttt
    17761 ttgcgcatcc taaatcagag cgtacgaggg catttttatc gcaggtaatc cattaattga
    17821 atgttagttc gaaaagcaaa aaggccatcc tttcggatgg cctttttgct tgatttgatg
//
LOCUS       DAZKHK010000105        17414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKHK010000105 DAZKHK010000000
VERSION     DAZKHK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.105"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..1456
                     /gene="tnaA"
                     /locus_tag="ACHOS5_003699"
     CDS             41..1456
                     /gene="tnaA"
                     /locus_tag="ACHOS5_003699"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP9778449.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1548..2792
                     /gene="tnaB"
                     /locus_tag="ACHOS5_003700"
     CDS             1548..2792
                     /gene="tnaB"
                     /locus_tag="ACHOS5_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP9778450.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSITAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIAVLAIKARKKFPNQMFTVWGGNLIPAIVI
                     LFGITVILCWFGNVFNVLPKFG"
     gene            2924..4099
                     /gene="mdtL"
                     /locus_tag="ACHOS5_003701"
     CDS             2924..4099
                     /gene="mdtL"
                     /locus_tag="ACHOS5_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP9778451.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLNFPWQSLF
                     WTMAIMGVAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4074..5033
                     /gene="yidZ"
                     /locus_tag="ACHOS5_003702"
     CDS             4074..5033
                     /gene="yidZ"
                     /locus_tag="ACHOS5_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP9778452.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5190..5939
                     /locus_tag="ACHOS5_003703"
     CDS             5190..5939
                     /locus_tag="ACHOS5_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP9778453.1"
                     /translation="MATHFARGILTEGHLISVRLPSLCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5961..6527
                     /gene="yieF"
                     /locus_tag="ACHOS5_003704"
                     /gene_synonym="chrR"
     CDS             5961..6527
                     /gene="yieF"
                     /locus_tag="ACHOS5_003704"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP9778454.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6581..7918)
                     /gene="adeP"
                     /locus_tag="ACHOS5_003705"
     CDS             complement(6581..7918)
                     /gene="adeP"
                     /locus_tag="ACHOS5_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP9778455.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIIANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8085..8750
                     /gene="yieH"
                     /locus_tag="ACHOS5_003706"
     CDS             8085..8750
                     /gene="yieH"
                     /locus_tag="ACHOS5_003706"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP9778456.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            8817..9290
                     /locus_tag="ACHOS5_003707"
     CDS             8817..9290
                     /locus_tag="ACHOS5_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFP9778457.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLMSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(9372..10094)
                     /locus_tag="ACHOS5_003708"
     CDS             complement(9372..10094)
                     /locus_tag="ACHOS5_003708"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9778458.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10109..11278)
                     /locus_tag="ACHOS5_003709"
     CDS             complement(10109..11278)
                     /locus_tag="ACHOS5_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP9778459.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11305..12921)
                     /gene="bglH"
                     /locus_tag="ACHOS5_003710"
     CDS             complement(11305..12921)
                     /gene="bglH"
                     /locus_tag="ACHOS5_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP9778460.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13007..14401)
                     /gene="bglB"
                     /locus_tag="ACHOS5_003711"
     CDS             complement(13007..14401)
                     /gene="bglB"
                     /locus_tag="ACHOS5_003711"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP9778461.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYQIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14420..16294)
                     /gene="bglF"
                     /locus_tag="ACHOS5_003712"
     CDS             complement(14420..16294)
                     /gene="bglF"
                     /locus_tag="ACHOS5_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP9778462.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKRQPAQVAQQEKTPEVITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGQIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16428..17264)
                     /gene="bglG"
                     /locus_tag="ACHOS5_003713"
     CDS             complement(16428..17264)
                     /gene="bglG"
                     /locus_tag="ACHOS5_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP9778463.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKILSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4425 a         4385 c         4075 g         4529 t
ORIGIN      
        1 acagggatca ctgtaattaa aataaatgaa ggattatgta atggaaaact ttaaacatct
       61 ccctgaaccg ttccgcattc gtgttattga gccagtaaaa cgtaccactc gcgcttatcg
      121 tgaagaggca attattaaat ccggtatgaa cccgttcctg ctggatagcg aagatgtgtt
      181 tatcgattta ctgaccgaca gcggcaccgg ggcggtgacg cagagtatgc aggccgcgat
      241 gatgcgcggc gacgaagctt acagcggcag ccgcagctac tacgcgttag ccgagtcagt
      301 gaaaaatatc tttggttatc aatatactat tccgactcac cagggccgtg gcgcagagca
      361 aatctatatt ccggtactga ttaaaaaacg cgagcaggaa aaaggcctgg atcgcagcaa
      421 aatggtggcg ttctctaact atttctttga taccacgcag ggccatagcc agattaacgg
      481 ctgtaccgtg cgtaacgtct atatcaaaga agccttcgat actggcgtgc gttacgactt
      541 taaaggcaac tttgaccttg agggattaga acgcggtatt gaagaagttg gcccgaataa
      601 cgtgccgtat atcgttgcaa ccatcaccag taactccgca ggtggtcagc cggtttcact
      661 ggcaaactta aaagcgatgt acagcatcgc gaagaaatac gatattccgg tggtcatgga
      721 ctccgcacgc tttgccgaaa acgcctattt catcaagcag cgtgaagcag aatacaaaga
      781 ctggaccatc gagcagatca cccgcgaaac ctacaaatat gccgatatgc tggcgatgtc
      841 cgccaagaaa gatgcgatgg tgccgatggg cggcctgctg tgcatgaaag acgacagctt
      901 ctttgatgtg tataccgaat gcagaaccct ttgcgtggtg caggaaggct tcccgacata
      961 tggcggtctg gaaggcggcg ctatggagcg tctggcggta ggtctgtatg acggcatgaa
     1021 tctggactgg ctggcttatc gtatcgcaca ggtgcagtat ctggtcgatg gtctggaaga
     1081 gattggcgtt gtctgtcagc aggcgggcgg tcacgcggca ttcgttgatg caggtaaact
     1141 gttgccgcat atcccggcag atcagttccc ggcacaggcg ctggcctgcg agctgtataa
     1201 agtcgccggt atccgtgcgg tagaaattgg ctctttcctg ttaggccgcg atccgaaaac
     1261 cggtaaacaa ctgccatgcc cggctgaact gctgcgttta accattccgc gcgcaacata
     1321 tactcaaaca catatggact tcattattga agcctttaaa catgtgaaag agaacgcggc
     1381 gaatattaaa gggttaacct ttacctacga accgaaagta ttgcgtcact tcaccgcaaa
     1441 actgaaagaa gtttaattaa aacactcaga gtggctataa ggatgttagc cactctatta
     1501 ccctacatcc tcaataacaa aaatagcctt cctctaaagg tggcatcatg actgatcaat
     1561 cagaaaaaaa acactctgca ttttggggtg ttatggttat agccggtaca gtaattggcg
     1621 gtgggatgtt tgctttacct gttgaccttg ccggagcctg gtttttttgg ggcgctttta
     1681 ttcttattat cgcctggttt tcaatgttac attcagggct attattatta gaagcaaact
     1741 taaattatcc cgtcggatcc agttttaaca ccatcaccaa agatttaatc ggtaacacct
     1801 ggaacattat cagcggtatt accgttgcct tcgttctcta tatcctcact tatgcctata
     1861 tctctgctaa tggtgcgatc attagtgaaa cgatatcaat gaatttgggc tatcacgcta
     1921 atccacgtat tgtcgggatc tgcactgcca ttttcgttgc cagcgtattg tggataagtt
     1981 cgttagccgc cagtcgtatt acctcattgt tccttgggct gaagattatc tcctttgtaa
     2041 ttgtttttgg ttctttcttc ttccaggtcg attactccat tctgcgcgat tccaccagca
     2101 tcactgcggg gacgtcttac ttcccgtata tctttatggc tttgccggtg tgtctggcgt
     2161 catttggttt ccacggcaat attcccagcc tgattatttg ctatggaaaa cgcaaagata
     2221 agctcatcaa aagcgtggtc ttcggttcgc tgctggcgct ggtaatttat ctcttctggc
     2281 tctattgcac gatggggaat atcccgcgag aaagctttaa ggcgataatc tcctcaggcg
     2341 gcaacgttga ttcgctggtg aaatcgttcc tcggcaccaa acagcacggg attatcgagt
     2401 tttgcctgct ggtgttctcc aacttagctg ttgccagttc gttctttggt gtcacgctgg
     2461 ggttgttcga ttatctggcg gatctgttta agattgataa ctcccacggc gggcgtttca
     2521 aaaccgtgct gttaaccttc ctgccacctg ctttgttgta tctgatcttc ccgaacggct
     2581 tcatttacgg gatcggcggt gccggactgt gtgctaccat ctgggcggta ataattgctg
     2641 tactggcaat caaagcccgc aagaagtttc ccaatcagat gttcacggtc tggggcggca
     2701 atcttattcc ggcgattgtc attctctttg gcataaccgt aattttatgc tggttcggta
     2761 acgtctttaa cgtgttacct aaatttggct aaatccttca ggaagccagc cattcgctgg
     2821 cttcttgcct gtcaggaaat cacttatgtc caaatggcaa ctcgcctgat tctccttcac
     2881 cacgtatgct ttgcgtcacc ttactatcag gacgctttag cccatgtccc gctttttgat
     2941 ttgtagtttt gccctggttt tactttatcc cgcagggatt gatatgtacc tcgttggttt
     3001 accgcgcatc gccgccgatc tcaatgccag cgaagcgcag ttgcatattg cgttctccgt
     3061 ttatctggcg gggatggcgg ctgcgatgtt atttgccggt aaagtggccg atcgttcagg
     3121 gagaaagcct gtcgccatac ccggcgcggc gctatttatt atcgcctcgg tgttctgttc
     3181 actggctgaa accagcgcgt tatttcttgc agggcgattt ctacagggac tgggcgcggg
     3241 ctgttgttac gtagtggcgt ttgccatttt gcgcgacacg ctggatgatc gacgtcgggc
     3301 taaagtgctg tcgttactca acggcattac ctgcatcatt ccggtgttag cgccagtgct
     3361 cggacatctg attatgctta acttcccatg gcagagtctg ttctggacga tggcaataat
     3421 gggtgtcgcg gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc ctgcggcccc
     3481 cgcagcttcg gacaaaccac gagaaaatag cgagtcactg cttaaccggt ttttcctcag
     3541 ccgtgttgtt atcaccaccc tcagcgtttc ggtgatcctc accttcgtca acacgtcgcc
     3601 agtattgctg atggaaatca tgggttttga gcgcggagaa tacgccacca ttatggcgtt
     3661 gaccgctggc gtcagcatga ccgtttcatt ctccacgccg tttgcgctgg gaatttttaa
     3721 gccacgtacg ttgatgatca cctcacaggt gttattcctg gcggcgggga tcacccttgc
     3781 cgtttcacct tcccatgcgg tttcgctgtt tggtatcacg ctgatttgcg ccggtttctc
     3841 ggtaggtttt ggtgtggcga tgagtcaggc gttagggccg ttttcattac gcgcgggcgt
     3901 agccagctcg accttaggta ttgcgcaagt ttgcggttcg tcactgtgga tttggctggc
     3961 ggcggtggtt ggtatcggcg catggaatat gctgatcggg attctgattg cctgtagcat
     4021 agtaagcctg ttgctgatta tgttcgtcgc gcctggacgc ccggttgccg ctcatgaaga
     4081 aatccatcac cacgcttgat ctcaatctgc tgctctgtct gcaactgctg atgcaggagc
     4141 gcagcgtgac caaagcggcg aagcggatga acgtgacccc ttcggcggtg agtaagtcgc
     4201 tggcaaagtt aagagcgtgg tttgacgacc cgctctttgt gaactcaccg ctggggctgt
     4261 cgcccacacc gctaatggtc agcatggagc aaaatctggc ggagtggatg caaatgagca
     4321 acctgctgct ggataaaccg caccaccaga caccgcgcgg cctgaagttt gagctggcag
     4381 cagaatcacc gctaatgatg atcatgctta atgcgctgtc gaaacggatc taccaacgtt
     4441 acccgcaggc gaccatcaaa ttacgtaact gggattacga ttccttagat gccattactc
     4501 gtggtgaagt ggatattggt ttttccggtc gcgaaagtca tccccgttcg cgggagctgt
     4561 taagctcgct accgttagcc attgattatg aagtgctgtt tagtgatgtg ccctgcgtct
     4621 ggttacgcaa agatcatccg gcactgcatg aagcatggaa tctggacacc tttttacgtt
     4681 atccgcatat cagcatttgc tgggaacaga gcgatacctg ggcgctggac aatgtgttac
     4741 aggagctggg gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt
     4801 ttatggcagc acagcccgac aatctgctac tggcgaccgc gccgcgctac tgccagtact
     4861 acaatcaact ccaccaactg ccgttggttg ctcttcctct cccgtttgat gaaagccagc
     4921 aaaaaaagct ggaagtccct tttacccttc tgtggcataa acggaacagc cgtaatccga
     4981 agatcgtctg gttacgggaa accattaaaa atctttacgc gtcgatggca taaccgaatc
     5041 gaatgaaacg cgacccaatt tcacaatgaa atgtaaaaaa gttgtaataa acttatctga
     5101 atcgaacttt tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc
     5161 ataatggacc attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa
     5221 cggaaggaca tctgatttct gttcgtctcc cctccctgtg tcatcaagaa gcccgaaaca
     5281 ttccccctca tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt
     5341 tcatgctgta tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggaaaaccgg
     5401 ttttcagcga taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggtcggcg
     5461 tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacacgcg
     5521 ggtttcatag cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga
     5581 tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct catcacgctg cgccgaagcg
     5641 tgctaaaact aaccggtgat gttttgaatg acgatccgcg cgatctgcaa ctactgccca
     5701 ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg
     5761 acgtgctggt gtggtccgtg gcggtcacgc ccacgattga aaagctcagt gtctgggagt
     5821 tagatggcaa acacggctgg aaaagcctgc ccgatattca cagccgcgcc aacaatccta
     5881 ccagccggat gatgcgtttt gcccagctct ctaccgtgaa ggctttttcg ccaaattgat
     5941 agacaactac aggagtcatc atgtctgaaa aattgcaggt ggttacgtta ctggggagcc
     6001 tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga
     6061 gcatggaaat caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac
     6121 agcaggaaga cggttttcca gcaacagttg aagctctggc ggaacagatc cgtcaggctg
     6181 acggtgtggt aatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg
     6241 ccatcgactg gctttcccgc ctgccggatc aaccgctggc aggtaaaccg gtattgattc
     6301 agaccagctc aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc
     6361 tggttttcct cgatgcaatg gtgatgaaca agccagaatt tatgggcggc gtgattcaga
     6421 acaaagttga tccgcaaacc ggggaagtga ttgatcaggg tacgctggac cacctgaccg
     6481 ggcaattgac cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc
     6541 cagcaatcat gcatggcggg tttttaacgc gctatcgatt ttagtgagcg tcgataaaga
     6601 caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac
     6661 gtccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcgata ccttcagtaa
     6721 tcgagaagct gaacggcatc atcacagcgg taataaacgc cggaacagat tcagtaagat
     6781 cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtaa atcagcgcac
     6841 cagctgcagc gtagcctggc accatccccg ccagcggcga caggaagata accagcagga
     6901 acagcagacc aacaaccact gccgtcagac cggtacgacc gccaacggaa acgccggaag
     6961 aagattcaat atacgccgta acagaagaag taccgataaa cgaaccggtc accgaagaga
     7021 tactgtcgac atacagcgcc tgcttcatgc gcgggaattt acctttctca tcagccagac
     7081 ctgctttatc ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accagcatga
     7141 aagagaaaat cacccctgcc agcccaaggt taaacgaccc ggctaaatct acatgaccca
     7201 cgacactcat tacgctcggc ggcgcagaaa cgatgccatt atagtggaca tcacccagca
     7261 tccagcccag cagcgttgtc accacgatgg aaaccagcac cgccgcgtga atattgcgcg
     7321 aggccagaat ggcgatgatg aagaagccga ggatccccag cagcacgctg tgagaagtca
     7381 gattaccgat gctcaccagc gtttccgggt tagcgataat cacacctgcg tttttcagcc
     7441 ccatcatgcc aataaacaga ccgataccac tggtgatgcc cacacgtaga cttaccggga
     7501 tgttggctat catccagtag cgaacgcgga aaatcgtcag taacagcaga ccaatcgcgc
     7561 cccagaagat tgcgcccatc ccgacttgcc acggcaagcc catcgcctgt acaaccacaa
     7621 aagcgaagaa cgcattcagg cccattgccg gtgccagtgc aactggcagg ttagcaaaca
     7681 gccccatcat aatactgccg aatgcagcaa tcagacaggt cgtgacgaag acggcgctgg
     7741 tatccatgcc agcaacgcca agaatttgcg ggttaacaaa aacgatgtaa accatcgtca
     7801 ggaaggtggt aaaaccggcg atcacttcgg tccgtgccgt cgtgccatgt tcgcgcagtt
     7861 taaacacgcg ttccagcatc ccctggccag aagcctgggt tgtgtgttgt tgactcatta
     7921 tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg agacaatatg
     7981 gacggctgaa gataactttt tttcattgta tttgcttata cggcaacgat tgcgtcatgc
     8041 gaattggcat gacgaaaacg ttaaactaac gataaaggaa agacatgtcc cagatagaag
     8101 cggtattttt cgactgcgac ggtacgctgg tcgatagcga ggtcatttgc tctcgcgcct
     8161 atgtcacgat gttcagggaa tttggcattc acgttgacct ggaagagatt tttacccgtt
     8221 ttaaaggcgt aaaactctac gaaatcatcg acatcatcag taaagaacag ggcgtgacga
     8281 tggtccaggc cgatgccgaa cacgtttacc gcgcagaagt agcacgtctg tttgataccg
     8341 aacttgagga catcgcagga gcgaacgaat tgctggcaag cattaacgtg ccgatgtgcg
     8401 tcgtctctaa cggtccggtc agcaaaatgc agcattcgct tggtaagctc aacatgctgc
     8461 actacttccc ggaaaaactg ttcagcggct atgatatcca gcgttggaag cccgatccgg
     8521 cattaatgtt ccatgcggca aaagcgatga atgtgaacgt tgaaaactgc attctggtcg
     8581 atgactccag cgccggtgcg caatcaggga tagatgccgg aatggaagtg ttttacttct
     8641 gcgctgaccc gcacaataaa ccgatcgttc acccgaaagt caccaccttt acccatcttt
     8701 cgcaattacc ggaactgtgg aaggcgcgtg gttgggatat tacggcatag ttattcacac
     8761 tcccttcact tactccactt aaattggcac gcaaaggtaa gtaaagggag tttgatatgt
     8821 ctgtttcacg tcgggtaatt catcacggac tgtattttgc ggttttaggg ccgttaattg
     8881 gtgttctgtt tcttgtcctc tacatattct tcgcaaaaga accgctgatt cttttggtaa
     8941 taatacaggt acttccattg tttctcttaa tgtcgataac tacgggagct attcctgcaa
     9001 tgttaactgg cgtaatggtc gcctgtctgc cggaaaagat cgggtcacag aaacgttatc
     9061 gttgtctggt tggcggcata ggtggagtcg ttatcaccga gatctattgc gctgttatcg
     9121 tacatattaa ggacatggct tcctcagcgt tatttgaaaa cattctttct ggtgaaaatc
     9181 tcgttgtccg catcatccca gcattgctgg caggcgtagt gatgagcaga atcattacac
     9241 gtctacccgg attggatatt tcatgtcctg aaacagactc tttaagctaa gcggaatgtt
     9301 ttatctattt gtcattaaat gttggagaac aggccggagc gtaattcaca catccggcct
     9361 tatttttaag cttaaccgag cgctaattct gcggcagcgg ctttatcagc gattaccatc
     9421 agcgaaggat gcagctgcaa cacggaagca ggaacatctt cggtaaccgg accttgcaga
     9481 acatttttca gtgcctgcgc tttacccgcg ccactgacaa ttatcagcaa gtttttcgcc
     9541 gccatgatgc ttttcggccc catcgtcacg tagctgtcag gcactaatga aaagtcgccg
     9601 cccaactcgc catgcgccac aatatcaacc atctctccct ggatcgggaa ctccacggtt
     9661 tgctcatgga aatgggtcgt atttggcaaa ttgccacaaa aatgaccatc tgcacctaat
     9721 cccagcacca ccaaatccag gccgccttca cgcgccagtt tctgatcatg ctcgcggtag
     9781 ttatcaatag tgagcttctg gatattctct tctttgatcc ccgcaggggt gaaaaacaga
     9841 ttacgcagat tggtaatcgt tacgccttct ccctctttgc cgcgaaatgg aatttcatcg
     9901 aaattataga aatagcagtt atcgtaccag ggcttacctt taaccagagt gatgagatat
     9961 tcgtacatgc ctttgggcgt gctcccggcg gtaattgcca ggttaacacg acgcgtcttc
    10021 gacatataac caagcagatg gtgtgccgcg acacggctca tttcctggta atcttcggta
    10081 atgattaatt tcatcttaat gccttatatt atttgaataa tttctgtaca aaggctgcat
    10141 aggctggtcg ccagacatcc atttcatggt tcagaccggg atattcctgg taatcaaact
    10201 taattttttt ctgctcaagc tcagttttca gcccggcgat atccttgccg gttacgacat
    10261 ctttatcgcc cacgaccaca gtaaaattac gtagttgctg gttaatagct gccggatcgt
    10321 tcagccgggc agcgacccct tcatctggta cggtcgtcgt ggtaacacca ctgaatgtgg
    10381 ccagccagcc aaagctttcc agatgattca ttcccgatac cagtgcctga tacccacctt
    10441 gtgaaagccc tgccagtgcg cgaccatcgg catctttacg gacattaaaa cgcttgctaa
    10501 tcagcgggat aatatcgttc atcagttcgc gatctgccgc tttagcattt aacggataaa
    10561 agactttacg tctctcctga ggaacgaaat cttcgggaat aatgcccttc gcatcggttt
    10621 cggtatccgg gatcaccacc agcatcggtt taattttccc ttcagccagc aggttatcca
    10681 tgatttgcgg gatacgcccc tgatcaatag cggaacgtcc ggtatcacca aagccgtgat
    10741 agaaatagag cactggcaaa ggctcgccta tgccggtgta tcctggcggg gtccagacat
    10801 acatctgacg ttcggattgc aatgcgttgg agtggtaagt tatggcgatc aaatcaccat
    10861 gcacaacaga acgtgtatcc agataactgc ccggtaccag aatcatactg gagttaacct
    10921 ggcgctgagg cttagtcatt gctgtacctg tatcaatgct gcgtacacca tcaacattga
    10981 aaaaatactc gtacaggttg cctttcagga caggcgtgcg ccacgaccag actcccgctt
    11041 catctttggt catcgggtga atattgtccg gaaccggaac acccaccact acggagacat
    11101 tttttgcccc aggggcaaag tagcgaaagg tcacgctgtt atcggtattg acctgagtca
    11161 catactgttt caccggaata gtgggtgccg ggctggctgg catatccgct gctatggccc
    11221 attgagcaga aataccgctg gcaatagcca gcgttaaagc agcaattttt atattcatcg
    11281 taatattcct tttattaagt tccattacca ccagatttca gcctgggcac cgacagcaaa
    11341 ctggtcgtct ttattgtctt caaaggtgaa gccatccagt tcgttttcca gagctttgat
    11401 atatgtagcg tagaaacgaa tttccgggcg cgaggtcaac atactggtat tgactttaaa
    11461 ggtatggaag agcgtggttt tataaccaga ctcattatat ttattgctat tcgcatcttt
    11521 gttttgctgg gtgaaatagc ccagttcaac acctgtctgg ttatattggt cccaaatata
    11581 agccgggcga acaaccgcac gaatagattc gaaatcagaa tgtgcgcctg tttcgtagct
    11641 ataaatatcg ttaccgaagg agtaaacaat cgcgttagcc acaatgaaat gatcgccgat
    11701 ataagcttcg ccctgcgaag tcagacgaac tgcagttccg cctgtgtgat caccataata
    11761 acgaccatta aaggtagtaa atggacttgc accagcataa cgaccaaagt tactggcgat
    11821 agagttattc gcaaccagga aggagaattc gttgaagcca cccttatcaa atttctgcgt
    11881 taaagatgta ccaaacatcc aggtatcttt ccagtcataa tacccgttat tatcctgatt
    11941 atctttttcc gatgcgcttt catttgctga gacataacga ccgctcacca ttaaggtggc
    12001 tttatcccat aacgggatat ctttatagcg taaatcaatg gtattggtat taatctgctg
    12061 tttgttttgc aggctgcgat cgtaatcatc gacatcttca cgaaccagcg cgatatcaat
    12121 tttacccgga ccgactttcc agttttccag acctacacct gcggcagcat cggtacgctg
    12181 cgttttccag tcaagcatct ggatttcaat tttcggcgca ccgtgtttac ccacccagaa
    12241 atcagcctct ggcgcaaagg gcaggaaacc tttggtggta acatacatat cagagaactg
    12301 cataaagttc tcgccaccgg cgttatcgcc aaaccagcca gtagagtact gctgacccac
    12361 gttaccatcc atcatcacga tggcatcaac ccgtttgccg ttttcgttgt agacacgttg
    12421 ttttaactgc aaatcaaacc agccggagta ttcgttacca aagcggccca gagaaccaat
    12481 tgcccatgat ttaggtgaac catgagaggc tgttccccaa ccagaacggt agtagccgtt
    12541 gtaactaaag ccgatttcat ctttcacaaa tttactgaaa tctttcagag tcatggaaga
    12601 ataaatcgag cctgtctcac tggcattttt ctcttcattt ttaaccagta cagcatcagg
    12661 ctttgcggca cgactggtcg ggaatggatt ggcggcatac ccttgatcat tcgtacttac
    12721 gctacgattc accgtcgctg gcgtatactt tttcttctct tcatctttat attttttcag
    12781 ttcgctttgc gtttctctta acgccttttc taatagctca aggcgttgtt ccactgttaa
    12841 tgattcagcc aatgattgtg cggaaaaggc taacggtgcc attaataaga tggcagaggt
    12901 aataatattt cgtctaaaca taatatccct ttatggtgca aagaaagaat taacgcatcg
    12961 catccagact gttctgaatg cgacgataat taaggtgctt taatggttat ttttttaatg
    13021 acagcccccg agttttgatc acttctgcat accatccgaa gcttttcttg cgtgttcttg
    13081 tgaggcttcc ttcgccatta tcatcacgat ccacataaat aaagccgtag cgcttagaca
    13141 tttgtgaatg agatgcactg actaaatcaa ttggccccca actggtgtac cccataatat
    13201 ccacaccatc ggcaatcgct tcatttacct gtaccaggtg atcgtttaaa taggcaattt
    13261 gataatcgtc ctgtatcgaa ccatccgctt caacgctgtc ttttgcgcct aatccgttct
    13321 cgacaataaa taacggtttt tgataacgat cccaaagtgt atttaacaga acccgtaatc
    13381 caaccggatc aatttgccac ccccactctg aacttttcag atgcggattg gggatcatat
    13441 tcagtatgtt gccctgcgca tttttattaa tgctttcgtc gtgggaaaca caaccggtca
    13501 tgtaataact aaaagagatg aaatctacgg tatgttttaa atcttctgcg tcactttcag
    13561 tcatctcaat ggtgatattg tggtcgcgga agaaacgctg catatagccg ggatactgac
    13621 cacgcgcctg aacatcacca aagaacatcc agcgccggtt ctcttccatg gcctgcaaca
    13681 tatcctgtgg ctggcaggtg agggggtaaa ccagcccacc gagaagcata ttgccaattt
    13741 tcgcttcggg gatcaggcta tgacaggctt taactgcccg cgcactggca accagttgat
    13801 gatggatcgc ctgataaact tccgcctcgc cactctcttc tgccagcccc acgcccgtga
    13861 atggcgcgtg taatgacatg ttgatttcat taaatgtcag ccataacgca actttatgtt
    13921 ggtagcgggt aaagaccgtg cgtgcgtaat gctcgaagtg atcgatgacc gcccgattag
    13981 cccaaccgcc gtagtttttc accagcccat atggcatttc gtaatgggat aacgttacca
    14041 gcggcttgat ccccgcctgc gccatttcat caaacagccg ctcgtaaaat gctaaccccg
    14101 cttcattcgg ttcggcttca tcgccctgag gaaaaattcg cgcccaggca atggaaatac
    14161 gcagacaggt gaagcccatc tcggcaaata acgcgatatc ttccgggtaa cggtgataaa
    14221 aatcgatggc gacatctttg atattctctt tccccaggat gcgcggttcc atttttccca
    14281 ttacgccatg aggctgtaaa tctgaggtcg tgatcccttt gccatcttcc tgccaggcgc
    14341 cttccacctg attggcagct gttgcaccac cccaaagaaa tgtttctgga aatgctttca
    14401 taattaactc cttttatcgt tagcgaatga tggataacag cggctcacca gcgtttatct
    14461 gcgccgtgcc gtggggtaat acgtccgtaa aatcatcgct attactgatt aataccggcg
    14521 tcgtcagatc aaatccggcc tcgcgaatag cagggatatc aaaagaaatc agccgatcgc
    14581 ctgtattgac cttgtcaccc acgttgacgt gagcggaaaa gaatttgccg tccagtttta
    14641 cggtgtcgat accgacatga atcaggatct ccacgccatc atctgactca atgccaatgg
    14701 cgtgtaatgt ggcgaacaac gaagcaattt gccccgccac cggagaacgc acttcaccaa
    14761 cagagggcag aatggcaata cctttaccca acaggccgct ggcaaacgtg gtatcagcga
    14821 cgtgaatgag cggcacaata tctcccgtca tcggtgaaca gataccgccc tgctcaggtg
    14881 taataacctc aggtgttttc tcttgctggg caacttgcgc tggctgacgt ttagcggtga
    14941 tgaaatgaaa catcaccgta ccgacaaatg cgcaaccgat ggcaatgaca ccgccaataa
    15001 cgctggccca tacggtgaaa tcaatccccg ttgacgggat ggtttgcatg aaggtgaaaa
    15061 tacctggcaa accaaaggag taaactttcg tttgcgcgta gccaatgatg gtggccccca
    15121 atgccccact tatgcaggcg ataacgaagg ggtacttacg cggcagattg acgccatata
    15181 ccgctggttc ggtgatacca aacaaacctg tcaacgccgc tgatcccgcc accacttttt
    15241 tctgtgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacttgc gccataatgg
    15301 cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgataatta
    15361 aaggtatcaa accccaatgc agaccgaaca tcacaaagat ttgccagaag ccgcccatta
    15421 ccgcgcctgc aaatgcagga accgcctgat aaagccagag ataaccggca gcaatcagtt
    15481 cacttatcca ggttgatagc ggccccacca gcagaaatgt gatgggcgtg atgaccatca
    15541 gacatagcaa gggtgtgaag aaatttttga ttgccgaagg taaccacgca ttaagtcggc
    15601 gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat
    15661 tcaacaatgt gaccggaata cccaggaaat ccaggcccag cgcatccact ttttgcccgt
    15721 tctcgaaggc agtcagaatt aatggatgca ctaacgctcc gccaatcacc atggcagtaa
    15781 atggattgcc gctgaagcgt ttccctgcgg tgtagcccag gattatcggg aagaaccaaa
    15841 acaaggcatc actggcgcta aataaaatta aataagtacc actttgttcg gtcgtccact
    15901 gaaaagtgag tgccagagcc aacatacctt tcaggatccc ggttgccgcc atcaaaccga
    15961 tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttaacaga ttacctttat
    16021 catcattttc cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttaaccgcca
    16081 ggaagacatc ggccacatgg ttacctatga caacctgaaa ctgaccaccg ctttccacca
    16141 ccataataat accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta
    16201 acttaaaacg taatcgcgtt gcacaatgca tcagactcac aatgttatct gcgcccccga
    16261 ctcctgcgac tattgttctg gctaactccg tcataacttg ccctctaccg ctttgcggca
    16321 aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg
    16381 ttttgcccgc ttagtgcggt aacaatcctt tattcagtaa taatatttca gtgttctttg
    16441 cgcacgcgct ctatatttat ggctaaaaac ataatctctg caggtgagat tttacgttga
    16501 tactgcaaac caataaaaat ggcgatccgc tccgcacatt gccatgcttg cgggtaattt
    16561 tgctttactg cttgttgtaa tgattcatca ctatcgttaa tcgatgcatg ttcaagaata
    16621 cgccaggata aaattttcag atgcgtaacc agtcgctgat aactcaagct ttcttcctgg
    16681 taattaaggc tgaactgaaa ttttattaat tgcagcattt ctcgcattaa ttgcgtgaca
    16741 cctgcaacat cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag
    16801 cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct
    16861 tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcag cgggttgggc
    16921 agtagcacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
    16981 taaatactgt cctgcaactt tcccaggcgc tcctgcgcca gagagataat acgatcacag
    17041 gttgccatca cctcaagagg catatgactt aagagttcgc ttaatcgccc gttcagttca
    17101 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gcctgggcgt
    17161 ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc
    17221 accacaacat tattgttgag aatcttggtg atttgcatgt tcatagcaag gacctttttt
    17281 ataaacaaaa aaacccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa
    17341 tgcagtaaca atccagtcat taattaatgg tttttataac gaacatccag gttcgaaaat
    17401 taatttagtt gcgt
//
LOCUS       DAZKHK010000106        17392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKHK010000106 DAZKHK010000000
VERSION     DAZKHK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.106"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..513
                     /locus_tag="ACHOS5_003714"
     CDS             <1..513
                     /locus_tag="ACHOS5_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9778464.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            529..1068
                     /locus_tag="ACHOS5_003715"
     CDS             529..1068
                     /locus_tag="ACHOS5_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP9778465.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ACHOS5_003716"
     CDS             complement(1161..2738)
                     /gene="guaA"
                     /locus_tag="ACHOS5_003716"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9778466.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ACHOS5_003717"
     CDS             complement(2807..4273)
                     /gene="guaB"
                     /locus_tag="ACHOS5_003717"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9778467.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLSQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4435..5811
                     /gene="xseA"
                     /locus_tag="ACHOS5_003718"
     CDS             4435..5811
                     /gene="xseA"
                     /locus_tag="ACHOS5_003718"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9778468.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTTVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVQQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5880..13931)
                     /locus_tag="ACHOS5_003719"
     CDS             complement(5880..13931)
                     /locus_tag="ACHOS5_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
                     /protein_id="HFP9778469.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPTKTHEVAFTAKPSEFNADVSVSKLLLTNPQDREGDIIAT
                     PRWENQTPPAVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKMTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEGGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNFYWSSLAGMSGQHHAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGINR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEATLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETDNELGSV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANAIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAKIAATTDRDVSAGSRAQAKVG
                     DTITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQSQGVGLSTPLNIVPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVFSPDGTLTENNEVWSRVSQANASSTSKG
                     GCGANMLPRRSQLSALYDANNGDGVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDIPFTLKRGDGYTRSEEKHVAGSSDALVAPVVVNGGLADETSLNNTAAAYS
                     AMTGSDGTKILTITRPDTHGTKTSLTARLYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGAVFKRPLLLKELSSTSGRTAIAEDNEDWAQFTQAQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDRMNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQLTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTLANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMTVIVRDSGGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGAINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDAATQAAKVKKGEAMSVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGNVV
                     AMDDLVVQPLSGSAVTLSESGNTISGMTGADGTASFSLRQDNTPGYKMPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNSKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14052..15029)
                     /locus_tag="ACHOS5_003720"
     CDS             complement(14052..15029)
                     /locus_tag="ACHOS5_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFP9778470.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELVTKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15084..17261)
                     /gene="sinH"
                     /locus_tag="ACHOS5_003721"
     CDS             complement(15084..17261)
                     /gene="sinH"
                     /locus_tag="ACHOS5_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFP9778471.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLVSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHN
                     FDKWLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPSYPQLGGKIVFEQYYGDEVALFGTDNLEKDPYAVTLGLNYQPV
                     PLLTVGTDYKAGTGDNSDVSINATLNYQFGVPLKDQLDSDKVKAAHSLMGSRLDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEAAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDQAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNIYRFVLWRDNNKDGVFQQVEKLTDEEMVQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4110 a         4614 c         4306 g         4362 t
ORIGIN      
        1 aaatttaaaa aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc
       61 cagtcaaatg ctgatgatca tgccgccgat gtttatcaaa ccgatcaact gaataccaaa
      121 caagaaacta aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac
      181 gcccaaaata aacggaacgc acaagccttc ggcgcgctta ttggcgctgt cgctggcggt
      241 gttatcggcc acaacgtcgg gtctggcagc aattccggaa cgacggcagg tgcagttggc
      301 ggcggagctg taggcgcggc agcgggttct atggtgaatg ataaaacctt agtggaaggt
      361 gtttctttaa catataagga aggcaccaaa gtgtatacct ccacccaggt gggtaaagag
      421 tgccagttta cgacaggttt agccgttgtt attaccacga cgtataacga aacgcgtatt
      481 cagccaaata ccaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt
      541 tttctttgcg ccatcttagc ctccttaagc tatccggcta tcgcctcatc attgcaggat
      601 caactatcgg ctgtcgcaga agcggaacag caaggtaaaa acgaagagca aaggcagcat
      661 gacgaatggg tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaac
      721 gcccaggccg cggctaataa aagagcggca acggcagcag cgaataagaa agctcgtcag
      781 gataaactgg acgccgaagc cactgcggac aaaaaacgcg atcaaagtta tgaagatgag
      841 ctacgcagct tagagattca gaaacaaaaa ctggcgctgg cgaaagaaga agcccgcgtt
      901 aagcgcgaaa acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg
      961 gtgcaatctg aagctgacgc aaacagaaat atgactgaag gcggtcgcga tctgatgaaa
     1021 agcgtgggca aagcagaaga gaataaatca gatagctggt ttaattaatc gatgttagta
     1081 acttcaatcc tatgattcgt gagtataaaa aaccctctgt agtaacagag ggttttgttc
     1141 attcatagtg cagagtcaaa tcattcccac tcaatagtcg ctggtggctt gccgctgatg
     1201 tcatacacca cgcgggaaat accattcact tcattgataa tgcggttgga aacgcgaccg
     1261 aggaaatcgt acggcagatg tgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1321 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtact
     1381 ggcaggaaga cggtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1441 tcaatgaaga tggcgtcagc acggcgcagc aggtcacaat actctttctt cacttctccc
     1501 agaacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacaacat gtcatacggt
     1561 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1621 accaggccca tcttcatctc tttcggcagg ccacccacgt tgtggtgaga tttgatgacg
     1681 tgtgctttac cggttgcaga agcagcagat tcgataacgt cagggtagat ggtgccctgc
     1741 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1801 cgcccgatga ttttacgttt tgcttccgga tcattttcgc cagccagcgc tgacaagaag
     1861 cgatcttctg ccggtacgtg aacaatattc agaccaaagt gatcgccaaa catatccaga
     1921 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     1981 ttaccgatag cgcggtgcaa cagcattgcg gttacggagg aatccacgcc accagagagg
     2041 ccgaggatga ctttatcgtc gcccacttgc tcgcggatgc gggctacagc gtcgtcgata
     2101 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2161 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2221 ttttcttcgt tggccatgat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2281 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacgtccag cagcggttta
     2341 ccatctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2401 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2461 gccatggtct gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2521 tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg ggccgccgga aagaataatg
     2581 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2641 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2701 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2761 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2821 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2881 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     2941 tcaggcccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3001 ctttataggc tacgcggcct tcgatacctt ctggcaccag tttgtcggca gcgttgtcgc
     3061 tctggaagta acggtcagaa gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3121 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacctg
     3181 ccagcatgga acctaccatc accgcgcttg cgccagcggc gatagcttta gcgatatcgc
     3241 ctgagaaacg gataccgccg tcagcgataa ccggaatgcc ggtaccttcc agggcttcta
     3301 ctgcatcagc aacggcggtg atctgcggaa caccgacacc agtcacgata cgcgtcgtac
     3361 agatagaacc agggccgata cccactttaa cggcactgca accggcttct gccagcgcgc
     3421 gggcacctgc agcagttgcc acgttgccgc cgatgatttg caggtcagga tatttagcgc
     3481 gagtttcacg gatacgctgc agaacacctt cagagtgacc gtgagaggag tcgatcagca
     3541 gaacgtcaac gcctgcggca accagcgcat caacacgctc ttcgttacca gcgcccgcgc
     3601 caaccgctgc acctacacgc agacggcctt gttcgtcttt acacgcgttc ggtttacgtt
     3661 ccgctttctg gaagtccttc acagtgatca tgccgatcag gtggaattca tcatcaacca
     3721 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcgc
     3781 cttcacgcac ggttaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggctca
     3841 agtcggtcac aaaacgcacg tcacgaccgg tgataatgcc gaccagttcg ttttcttcgg
     3901 taacaaccgg gtagccagca aagccgttac gctcggtcag ttctttcact tcacgcagcg
     3961 tcgtggttgg cagtacggtc tgcggatcgg ttaccacgcc agattcgtgt tttttcacac
     4021 ggcgaacttc ttctgcctga cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4081 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4141 gcatagggat attcagacga atagttttcg tcagttgggt gctgaggtca gcagtattcg
     4201 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4261 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4321 tacagagcgt aaccgattgc atctaccctt ttttgcaaaa aatgcttgct atccccggag
     4381 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4441 ccttctcaat cccctgcaat ttttaccgtt agccgcctga atcaaacggt tcgtctgctg
     4501 cttgagcatg agatggggca ggtgtggatc agcggcgaaa tctctaattt tacacaacca
     4561 gcctccggtc actggtactt tacgcttaaa gacgataccg cgcaggtgcg ctgtgcgatg
     4621 ttccgaaaca gcaaccgccg ggtcaccttc cgcccacagc acgggcaaca ggttttagtt
     4681 cgcgccaata ttacactcta cgagccgcgc ggcgattatc aaattatcgt cgaaagtatg
     4741 cagccggccg gtgaaggatt gctgcaacag aagtacgaac agcttaaagc gaagttgcag
     4801 gctgaaggtt tgttcgatct gcaatacaaa aaatcactcc cctcccctgc gcattgcgtt
     4861 ggtgtgatca cctcaaaaac cggcgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4921 cgcgatcctt ctctgccggt gattatctac cccaccaccg tacaggggga tgatgcgccg
     4981 gggcaaatcg ttcgtgccat tgaactggcg aataagcgca acgagtgtga cgtgttgatc
     5041 gttggccgtg gcggtggttc gctggaagat ttatggagtt ttaatgatga gcgtgtggcg
     5101 cgggcgattt ttgccagtcg cattccgata gtcagcgccg tcgggcatga aaccgatgtg
     5161 acaattgctg actttgttgc tgatctacgt gcgccaacgc cgtcagccgc cgctgaagtg
     5221 gtgagccgaa atcagcaaga attgctacgt caggtgcaat cgacccgtca acggctggag
     5281 atggcgatgg attattatct cgccaaccgc acgcgtcgtt ttacgcagat tcatcatcgc
     5341 ttgcagcaac agcatccgca gctccggctg gcacgtcagc aaactatgct cgaacgtctg
     5401 caaaagcgga tgagctttgc gctggagaat cagcttaaac gcgcgggtca gcaacagcag
     5461 cgattaacac ggcaactcgt ccagcaaaat ccgcagtcgc gtattcatcg cgcgcaaacg
     5521 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgcacagct tagcgccacg
     5581 cgtgaacgtt tcggtaatgc agtaacacac ctcgaagccg taagcccact gtcaacgctg
     5641 gcgcgcggtt atagcgtcac cagcgccgct gatggcgcgg tgttaaaaca ggttaagcaa
     5701 gtgaaagtgg gtgagacact gaccacacga ctgggagatg gtgtggtgat tagtgaagtc
     5761 agcgccgtaa caaaaacccg caaatcacgt aaaaaaacgt ctaatccgta gaccggataa
     5821 gaggcgccag catcgcatcc ggcaataact cccaggccac gccagatccg ccatgctggt
     5881 tactggaagc aggtcaccag acccggttta tttattagcg tgatactaca atcctgtgct
     5941 ccggtacctt cgtccatccc acactgttga gaggacaaat aaggaaaact gggtgaagac
     6001 ggccatccca gtgaattaaa aacagtatgc aacaactcta attcattgta tgtcggtgca
     6061 ttttctatgc tgccacactg ccttgcgata tcccatttac tggcatcaat gatatgtgca
     6121 gaagcccagt tttcgtagtt aataatcggt gaggatacgg gattagcccc ggacggcaat
     6181 tccgtcgtta acaacggtcg atgtagcgat ttactattca ccactaccgt gtctgccata
     6241 tgtccccaaa agtgcgccga ggaaacattt gggctggttg gcaccgtaaa aatagcgtcc
     6301 agcgtgtctg tcgcggaagc gtaattagcc agagtaaccg tcagtggcat tttataacca
     6361 ggggtattat cctggcggag cgaaaaactt gccgtcccat ccgcgcctgt catgccacta
     6421 atggtattac cgctttcact cagcgtaaca gccgaacctg ataacggctg aacgacgaga
     6481 tcgtccatcg ccactacatt gccatacaac gttgccccag cgttacgcgg cgatgcctca
     6541 ccacgtttca aagtaaattc aacattcggc accgtattcc cggcgctgtc tttcaccgtc
     6601 acggtaacgg acatggcttc gccttttttc actttcgccg cctgagtggc tgcatcaaac
     6661 gctgttgagg taagtgtgac gctggaaacc gttgaacgcg cgttagccag gcaaggctga
     6721 aatgattttg ttgaacccgt atacagcgtg ccatttctca aatcaaccga ctggtcttta
     6781 ccatcggcgg atttatcctc tgcccaccag gtgtaatacg caggccagcc agtttcagtg
     6841 acaaacgtgt tattcggata acgcgcagac aacgaactta aatcattgaa tagtggctgc
     6901 cttgctactt cacaagggct cacactcgca tctgatttct gtgttggggt aaagtacggc
     6961 cagtcttcat tattgttatt gatcttgccg acgccagaag tcgatgtcag ttcactccac
     7021 aatttcgggc gttcaaatac cgcaccattc actgccgtca aggttgctgg catatgcccc
     7081 cagtagttag ccttcgcaga atccgggctg gtcactaccg tgaatatcag gtcctgatta
     7141 gccgtaacct gcggatttcg cgccaatgaa gctgtaaacg gcgttaccag accaacggtg
     7201 ttattttggg taacgttgaa cgttatcgtg ccatcactgc cggtgaagcc gctccatgcc
     7261 gacgtattct ggttgaagga gatacgcgcc gatgtcgggg tgacttcatc taaaatgaga
     7321 tcatctacgg tattggagga tacagagatc cctgaacggc ttttcgcctg cacgcgcttg
     7381 agcgtaacac cttcgccgcc aacaggatta ccattggcat ctttgatcgt caccacgatg
     7441 ggaagcatct cacctttttt cacagatgac gtgggggtac caccattgct ggcagttcgg
     7501 ccctcatcat aggcagtcga tgacagcgta aggcttgctg gtgccgaatg tggctgcgtc
     7561 agacagagca ttaaagcgta tccatttata gtcgtcgtcg attgtcccgt tttcaaatta
     7621 atggcgccgt aataccacgt caacgcgtca gtggtgctca tttcataagt ccaccaattt
     7681 ccacttccgg cagttatagg caaccccaaa tctgtcagta ttgtcccgcc tggatgatca
     7741 ttatatagcc cctgtaactc attgagacgc ggctgatact caaccgggca atccggcgta
     7801 actttattgg tataaaaaag gtatgaccac gactcattat tattacttac cgaggacccc
     7861 aaggtcgctt ctgctgccag tagcggtcgt ttaaaggtca cccccgccct actggtaaac
     7921 gtgtcgggca tatgacccca gtattttgct ttcggtgagt tcggactggt tatcacagtg
     7981 aaaataacat ccatcgtggc actttttgtg gtgtcgcgca tcaatgttgc agtgagcgga
     8041 gttgccagcc cataagtctc gttttgcgtc acatcaaaag tcgctttacc atcacttcct
     8101 gtcgtcagaa gtgcctgtgc cccattatta tagagtgaca tgtttactcc ggcaggggtg
     8161 accggcacga tagtaagatc atcatacgta gaatctagcc tgttttttgc acgatccaac
     8221 gcagtcccgc gtatgagatt aaagtttgcg cctggcaaca gatttcctcc gctatcccgc
     8281 acaatcaccg tcatccggat cgccgtacct ttcgccgccg ttgcactagg gcgaccatcg
     8341 ttgctggccg ttttctccgc atccagtgcc gtggaggtca tctctataga agcagccact
     8401 gcatgagggt ttaccagaca ggacacaaat gccacggtgt ttgataatgc cctggagctt
     8461 gatccattcg ataaattaat agactgatcg taccagacgt tcgcgtaggc aattttatcg
     8521 taagcccacc aggtgttagt cagcggaagg cctaaatcgg tccctatcgc accattagga
     8581 tgttcactgt ataactcttg aaattcgctt aataacggtt gataaggttc gccgcagtct
     8641 gatttgctga catcattttt tgctgccgta ttcaaagaaa gccatgattc attattagcc
     8701 agggttgact gccccgctgg atgctcggca gtaagcaatg gccgcttaaa ggcaattccc
     8761 gaccgggtag taaacgtttc aggcatatgc ccccagtaac ttgccagtgg cgtatccggg
     8821 ctggttatga cggtaaacat gaccggtaat ttgttggatt ctatcgtcaa ctgatacaac
     8881 tgggcgtaca tattggttac caggcccata gaatcatctt gttttaccgt aaaggaggta
     8941 gtcccgtccg cgccagtcac cccataaagc aggcttgaag aactattcat cctcgcagaa
     9001 gcgcctgccg cgttaattac cgtaatcgac ttatttatca tctcggcatt ttgacggttc
     9061 ttcgagcctt cacgtctgag aataaagggg acattgggca cgctatttcc ctgcgcattt
     9121 ttagtcacca cgcgaatcac cgcgtcttcg ccttttttca ctttcaacgc actcaaggtg
     9181 tcgtcaactt tgacaaattg cgccgtatca ttcgcctcaa caatgatctg actggcaacg
     9241 gcgacaggcg tggtccggca gctcagtaat gcaccaccag atgagggcat ctgcgtttca
     9301 tctcctgtct tcagcgaaac gacatcatac gtaaaggttt gagtgaacgt atctgcaacg
     9361 gtattcgaga tatagaacct cgacactggc cagccaatag catcatgtaa tgcgctactc
     9421 ccctggtgcg cataaatggc cctaagttca ttgtcagttg gaacatactc cgcgccacaa
     9481 ccatttatcg aggtatcgtt agcctggccc gacgggaacg tagaccaggc ctccccctct
     9541 tccaccaggg taccgatact ggtggtagca ctattctcgt ccctcagcaa aggccttcga
     9601 taaataagac cgtcatcgcc tgtcgccgtt tcagccatat gcccccagaa tttggctttt
     9661 ggcgaatccg ggctggtaag caccgtaaag acaacgggaa gcgtcgattt agtgctgctg
     9721 gaggaatcca acgctgccgt gagttctgtt tttagcccgg tagtctggtc ttgcttcaat
     9781 gtgaaagtgg tgatgccatc agcacccgtc acgccataaa taaccagggt cgttgaaaga
     9841 aaatcatctt gagtatttcc ccaggcatcc gttaccgcca gcgaagcaat cgacgtcgta
     9901 gttgacacat tggccctgtt cacgctggtg tttcgtttga gggtaaatgc ggtatttccc
     9961 acagggttac cctgcgcatc tttagtggtc actcgtaata cggcttcttc gccttttttc
    10021 gctttgacaa cattattcat acggtcaaat tttgtggggt ctgcagcttc aagaacaatt
    10081 tggcttgcct taacaatcgg cgtcgtctgg caggtaagca gttcttttgt ggcagatgga
    10141 attatcgaac tggtgcctga actgagattg acggccttga agtcacgctg ttccaggcta
    10201 ctactacctg tcgtgctgga aaggtaactt gaggcgacgg gccagccctg aaccgttttc
    10261 atcgcatttc cgcggttggc ttcatacaat gactccagcc cggcctgaga gggaacatat
    10321 tcactaccac aaccattaga ggatgtgctt atagcctgag cttgggtaaa ttgcgcccag
    10381 tcttcattat cttccgcaat cgctgttcga cccgacgtac tggataactc tttcaacaat
    10441 aatgggcgtt taaagaccgc tccatctgca gccgtcactg tttcaggcat atgcccccac
    10501 attttcgcct tatcgctgtc cggactggtt acaacggtaa aaatggtatc gagggtcgct
    10561 tttttcgttg tatctgaata gagcctagcg gtcagcgacg ttttcgtgcc atgggtatcc
    10621 gggcgggtaa tagtgagaat tttagttccg tcgctgccgg tcatggcgct gtatgctgca
    10681 gcggtattat taagcgaagt ttcgtccgcc aggccaccat tgaccacaac cggcgcaacc
    10741 agcgcatcac tgctaccagc aacgtgtttt tcctctgagc gggtataacc atcgccacgt
    10801 ttcagggtaa agggtatatc acccaatgga ttgccctgcg catcttttac cgtcaccttt
    10861 acctgtagcg tttcgccttt tttcagcttc gccgcatttg cagccgcgtt ccactgcgcc
    10921 ggatccacca cttccagcgt aatgctactg gcaggattgt tagccgtcgt caggcaactg
    10981 acataaaccg ccgtgctacc gccaacggtc cgcgccccat cgttgagggc tatggtgtaa
    11041 taatgcggta cctgatctgc gggcgaactg ctccagtagg gctgacgctg cgttggccag
    11101 ccgtgtactg tctgtacacc gtcaccgtta ttggcatcat agagcgcgct aagctgagaa
    11161 cggcgtggga gcatattggc gccacagcca cctttactgg tgctggaggc gttggcctgg
    11221 ctgactcgcg accagacttc gttattttcc gttaatgtgc cgtccggact gaacacttcc
    11281 gcagccagtc ggggtcgggc aaaggttaaa gcatcgacgg taatcgtctc atccatatgc
    11341 ccccacatct gcgcgccaac cgcatccgga ctggtgagcg tagtgaaaat cacattgtaa
    11401 ttaacagtat tgggaatata ggagttcaca ggcacaatat ttagcggcgt cgataagccc
    11461 acaccttgcg attgcttaat ttccacagtc gcaaaaccct gcgcatccgt agtaccggca
    11521 tagactaaag acggttgcga tgttccgtag agtgtgccat tcatctcgat tgcgccgcgg
    11581 gttggatcat caaaacctgt agtctgcccc tgacgatttt tccccatatc tttagtgatg
    11641 gtgaacgcgg tatacggcac gggtgcatta ttcaaggcat taaaggtgcg taccgtcatg
    11701 gtgatcgtgt cacccacttt ggcctgcgct ctgctgcctg cggaaacatc acgatcggtg
    11761 gtcgcggcaa tcttggcgac catctcattg cccgaacaag agagatagtt ttgcttaaag
    11821 ccggaatagg tgtcgatgtt gcccgtggcc aggtttaccg acgcgtgcga cgtctcttcc
    11881 accgcactaa gatacgcttt ttgcaacgtt ggccagccat attcggtgcc gatggcattg
    11941 gctggatgag cggcaaacaa actgtgcaat gaactttggc tcggaatatg acctaaacca
    12001 cactcagcct gcatactgct attctgatca aacagcgccc agtcttcgtt attttcccgc
    12061 accgatccca gttcgttgtc ggtttcatcc gccagccgtg ggcgtttaaa gatattgttc
    12121 gcttcaataa tgcccaacat atggccccac atccgcgctt tgtcggtatc agggctggtg
    12181 atcaccgtaa aaataacgtc tttctcatcc gaggcagtga aatcactgcg cattctcgcg
    12241 gtgatgtgag ttttcacccc cacgccgcct ggctgggtca gcgtcagagt ggcctctccg
    12301 ttagcatcag tgatcccttc ataggtatgg gcgtcaactt tgcgaacgtt actgccgcca
    12361 tcaatttgta ccggatgcgt ctcccacgcg gcgtcagttt gattcttacg gttaatgccg
    12421 tcatccagat gcagcgaaaa gtagtagtaa gccagcggct gatcgttgtt cttgctatcg
    12481 gtaatcgtca gacgtaaaga agcctcttcc cccaccttta ctttcatcgc ttttatcgtc
    12541 gaatcatagc tgcccggcgt cagtaccagc ttcggttcaa catcgggcgc ttctttatcg
    12601 acacagctca caataaacgt cgtatcgtcc ggtttttgcg cttcgctgcg gttcgaaaca
    12661 tccgccgcat ggtgctggcc gctcatcccc gccagtgatg accagtagaa attaccctgc
    12721 atcggccaac cagccacatc ctgcactgac gttggcacca ccgtcgccag cgcaccgaac
    12781 tgacgcattc cgggcaaaat gtcgcaatgt ttatccgcgc cgctccaggt aaaggtggac
    12841 caggtttcat tgtgatcgtt aaccgtaccg ccttcattat cgacttccgc cgccagtttt
    12901 ggtcgactaa aagtgtaatc acgggccttc agcgtatcgg gcatatgacc ccacattgta
    12961 gcctgcggaa catccgggct ggtgaggacg gtaaagatca ctgccgtttc actggcctga
    13021 gcaatacccg cgaggctcgc gaccaacggc gtttttacac cagggccgtt ggcctgggtg
    13081 accgtaaccg tggcaacacc attcgcatcg gtagtaccac gatattccgt cgcggtggtg
    13141 gttaattcgg tcgtgcccag ttttacaggc gcagtattgt tcaccacacc ctggcggttt
    13201 tcagcatctt tgcgtttgat aacaaaagga atattcccca caacattacc gacgcaatct
    13261 ttggtggtga ccgttaacgt gatactgccc ccaaccgtca ttttcgccgc acctgaggat
    13321 tcgtcatagc ccgcagcgct gacggaaata aggtcaccct gcgcgggcgc aatcttcagc
    13381 gtcactttct gatcggcgag cgtcagaaca ctttcatagg gatagcccgt cgaggtgagt
    13441 aaatataaca gcggcatggt ctgctgggga tcaggctgcg cccaggcgac cagcgaacgc
    13501 ccggcaagtc cctgtgcaca aaacgagcga tctgcgactg gctgtgggtc cagtaaggtg
    13561 tccggcgtgg cggcatccgc ccaaactaaa gatacagccg gtggcgtttg gttttcccaa
    13621 cgtggggtgg caatgatatc gccttctctg tcttgcgggt tggtgaggag caatttagag
    13681 acgctaacat ccgcgttgaa ttcactcggt ttagcggtaa acgctacctc atgcgtcttt
    13741 gtaggatcca gaaagacact gccctggtag acgggtatgc tggcgctgtc ggcggtgggc
    13801 acggtaccgt taaattcgcc gaccgtatct gaagagtctt gccatgcaga ttctgcccat
    13861 gaccacccag gcagcattgc gcttatcagc aatgcgcata acacaaggat tttattcagg
    13921 gtcagggaca tgacagaaac tccaccggtt gttaaacgga cgcttgtcgc atcaacatat
    13981 tttgcttaat ccgggattaa gcaaaatatg cggaaacagc attcccttgc gggaatgctg
    14041 ctgagtacgc gttatttgtg tttgtaaata atgtgaagtt tgtaaccctg taccccgtcg
    14101 ccgttggtcg ggatggaaag tgcctctttt ccatcgcgcg cagggccgtt aaggttggtt
    14161 ctggcttcgt cgttggtcgc agggatgacc aaattgttat tatcgaagct tgagttaacg
    14221 atgccacccg aagtgccaag ctgttggctg ctgccagaaa gtacccaacg gaagtcataa
    14281 ttggcggtga cgtcagcatc ggtacccgca tcaaacttgc catttttgtt ctcatccgag
    14341 tacagtttgg ccacgtaggt tttgccaagt tcgattggcg tgctaccgtt gataaggttg
    14401 atactggtac catcaacaaa gatggcgact ttcaggttct ggttaaccac cggccccggt
    14461 gcaacgttat cgctgtcgct tccgccgccg ctggcagtag aaacgtcata aagatgcagc
    14521 gcagtcccga cgtttggcgt accggtctgc gtggttggcg taatttcaat accgatataa
    14581 cgaccacggt cggcatcggt aagagtgtaa ctggtcacct tcgttaccag ctcttttgga
    14641 tcacccccct gcgcatcctt gtagctcatc cagcggatag tatcgatagt tttcgcgtta
    14701 tcgcccaatc ccccaagatc cacgtcgcct tcggtatcac caatttccca acttacggtt
    14761 aatttatcac ccgagtaaag acgcttgtcc gtttcactgc cttgcgggcg agcgcctttt
    14821 agatcagaag tcaacgttac acggtcggca tctgtcgtag cacttccccc ttccgaatag
    14881 agccacggaa tggtcccctg gaaaatattg gtttcaccgc tgtcgaccca ggcgctgtct
    14941 tcagccagcg ccacaggtgc cacacaataa cctgccagtg ccagcgctaa tgcgatccgg
    15001 gtcagaccgc gtcttttatc ctgcttcatt gatgctctcc ttaatgaacc ttcagcgact
    15061 ggccgactgg ccttacgcca atcttatttt ttggtataca gcacgcgtaa accgtacccc
    15121 tgcaagccat ctcccgcttg tgcgccatag gtttgcgccg cttcacggtt agtggccgga
    15181 atcacaatgt cctcattgga agtgttcgcc tgtgcaccca cttcaccatt gatgctcttg
    15241 ccggtaaatt cccacttgta gtcgtactgc accatctctt cgtcagtcag tttttcgacc
    15301 tgctggaaga caccgtcttt attgttgtcg cgccataaaa caaatcgata aatgttgttg
    15361 agcggcaacg gttctttatc tttacctatc aggttgctgg gtttagccgc ccccactcga
    15421 tagataaaag catttagccc accaatttct gccccgagga aagtgacatc cacatagtta
    15481 ctgtcatcgt agggcgcggc ttttgctttc cagcgatagg tgccgggtaa ggtactggag
    15541 agggtgacag tgcccttttc tttatcttgt ttggcaataa aaacgcaggg gttatcggtc
    15601 gagcagggct tgttggttaa cgtcactcgt ttgtcctgcg cgtcgtaaag ctgaggcgta
    15661 aggttatctt ccggcaacgc atcgccgttg cggtcggtgt tatcgccaaa gctgtcggtt
    15721 acatcgagat ccatcactac accatctaca ccatcggcct gtgcgctggc ttcgtggctg
    15781 tggttgttct cttctggcac gttcgcgatg ttattaacaa tcaactctat tttacggtcc
    15841 tgctgaacgg tgatttccac cacgccagag ttctgtcggt tgcccttttc atcttccagc
    15901 gccagacgaa cccgccaggt attgagtttt tcttgttcgg cttgatcgga actgtactgc
    15961 caggcaggca gcaccaggtt ccagtggtta ttgttaccct ctgtcagagt attctctttg
    16021 ataaccggca tcaccagcgt gcgggcagca ttgtttttcg cctgccagga ggcagcaacg
    16081 attttgtaat gatgattaat gagtgcgtta atggtccact tacatttttc cagaccgacg
    16141 gccgcttcgg gagtgtcctt aatgacgcac tcagggtgct cgttggtggc atccgctttc
    16201 aaccacaggg tgacatcaag cggatctttt tctttgtatt ccagaacaat aaagttatta
    16261 cgctcaacga aatcaagacg gctgcccatc agcgagtgcg ccgctttcac tttatcgcta
    16321 tccaattgat cttttagcgg aacgccgaac tgataattaa gagtggcatt aatgctgaca
    16381 tcactgttat ctccggtccc cgctttatag tccgtcccaa ctgtcagtaa cggcactggt
    16441 tgataattca gtccaagcgt taccgcgtag ggatctttct ccaaattatc cgtaccaaaa
    16501 agggcgactt catcgccgta atattgctcg aagacaattt tgccccccag ttgcggataa
    16561 gaaggtaacc aggcttcggc acgaatatcc caaccgcgcg cagggcgttc ttcataaaag
    16621 tcgaaatctt cagagtcttt ccagtcagaa agtgggtgat aatagttgcc tgagaatttt
    16681 aaataatccg tccaggcttc ggcgcctaaa cctaaacggc gatgtccacg ggtaaagtca
    16741 taatcataaa atatatttcc acccaataac catttatcaa aattatgtct tacaccaagc
    16801 cctatatttc cgatcgtacg atcttctttt cgttgcgcag aaaattggct gaaataaaca
    16861 gtggtttgat tatcgtacca gggaacaaaa taatcgatgg aactgccatc cagatcgccg
    16921 ccctgaccaa tggagaggtt cgaacggacc ttaccgtagg gagagagtgt gtcttcaata
    16981 taagattgtg cggttgaggt gatttgactt tgcagatagc ttctggcctg agattccagc
    17041 gcctcagggg tcaagttttc aaacccttgc gtggcggagt tgatgacgta atcggctccg
    17101 cgctctttca gcgatttacc cttgttggtt tgttcatcct gttgtgctgc ctggctaact
    17161 aaatcaggta acgattcgtt ttgcttaaca tttatctctg gcgccgcgaa agcagcacca
    17221 gagataaatg ttagaataat acagcgtttc catcgcagca tgcactttcc ctgtgacaat
    17281 gatacagtca aaaataagct ttaatattgt atccctgatt attcagaaag gcatgtcata
    17341 aagaataaat atattcttaa ttgtttaaat tagtgatctc actcaattgc at
//
LOCUS       DAZKHK010000107        17354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKHK010000107 DAZKHK010000000
VERSION     DAZKHK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.107"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(91..822)
                     /gene="rlmB"
                     /locus_tag="ACHOS5_003722"
     CDS             complement(91..822)
                     /gene="rlmB"
                     /locus_tag="ACHOS5_003722"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9778472.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(913..3354)
                     /gene="rnr"
                     /locus_tag="ACHOS5_003723"
     CDS             complement(913..3354)
                     /gene="rnr"
                     /locus_tag="ACHOS5_003723"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9778473.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3393..3818)
                     /gene="nsrR"
                     /locus_tag="ACHOS5_003724"
     CDS             complement(3393..3818)
                     /gene="nsrR"
                     /locus_tag="ACHOS5_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP9778474.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4023..5321)
                     /gene="purA"
                     /locus_tag="ACHOS5_003725"
     CDS             complement(4023..5321)
                     /gene="purA"
                     /locus_tag="ACHOS5_003725"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9778475.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5425..5622)
                     /gene="yjeT"
                     /locus_tag="ACHOS5_003726"
     CDS             complement(5425..5622)
                     /gene="yjeT"
                     /locus_tag="ACHOS5_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP9778476.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5704..6708)
                     /gene="hflC"
                     /locus_tag="ACHOS5_003727"
     CDS             complement(5704..6708)
                     /gene="hflC"
                     /locus_tag="ACHOS5_003727"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP9778477.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6711..7970)
                     /gene="hflK"
                     /locus_tag="ACHOS5_003728"
     CDS             complement(6711..7970)
                     /gene="hflK"
                     /locus_tag="ACHOS5_003728"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP9778478.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSTTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8056..9336)
                     /gene="hflX"
                     /locus_tag="ACHOS5_003729"
     CDS             complement(8056..9336)
                     /gene="hflX"
                     /locus_tag="ACHOS5_003729"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP9778479.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9412..9720)
                     /gene="hfq"
                     /locus_tag="ACHOS5_003730"
     CDS             complement(9412..9720)
                     /gene="hfq"
                     /locus_tag="ACHOS5_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP9778480.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9806..10756)
                     /gene="miaA"
                     /locus_tag="ACHOS5_003731"
     CDS             complement(9806..10756)
                     /gene="miaA"
                     /locus_tag="ACHOS5_003731"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9778481.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDDVLQVVGAIAG"
     gene            complement(10749..12596)
                     /gene="mutL"
                     /locus_tag="ACHOS5_003732"
     CDS             complement(10749..12596)
                     /gene="mutL"
                     /locus_tag="ACHOS5_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9778482.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12606..13943)
                     /gene="amiB"
                     /locus_tag="ACHOS5_003733"
     CDS             complement(12606..13943)
                     /gene="amiB"
                     /locus_tag="ACHOS5_003733"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP9778483.1"
                     /translation="MMYRIRNWLVATLLLLCAHVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13962..14423)
                     /gene="tsaE"
                     /locus_tag="ACHOS5_003734"
     CDS             complement(13962..14423)
                     /gene="tsaE"
                     /locus_tag="ACHOS5_003734"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9778484.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14395..15942)
                     /gene="nnr"
                     /locus_tag="ACHOS5_003735"
     CDS             complement(14395..15942)
                     /gene="nnr"
                     /locus_tag="ACHOS5_003735"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP9778485.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGTAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15941..17080
                     /gene="queG"
                     /locus_tag="ACHOS5_003736"
     CDS             15941..17080
                     /gene="queG"
                     /locus_tag="ACHOS5_003736"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9778486.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17063..17116)
                     /locus_tag="ACHOS5_003737"
     CDS             complement(17063..17116)
                     /locus_tag="ACHOS5_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778487.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3951 a         4947 c         4351 g         4105 t
ORIGIN      
        1 aaatgaatat ttgcagtacg taattatctt accagctata gacaaaaaaa aaccatccaa
       61 atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg cttcaaataa
      121 gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca ttgggatgct
      181 gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat
      241 caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc
      301 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg ccagattagt
      361 cacccgaatc agcggaacgc tttctgccgc accacaggcc acttttttcg ccgtggcgtt
      421 gagctgtgcg gagcgatctt tcggcacaat caccgcatga acaccagcgg cgtccgcgct
      481 acgcaggcac gcgccgaggt tgtgcggatc ggttacgcca tcgaggatca gcaggaacgg
      541 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgcc gtcctggctt
      601 cacacgagcg ataatgccct gatgcacggc accgtcgctt ttctcatcga gatattggcg
      661 gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg
      721 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
      781 cagggcctgc actgcatgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
      841 cgttttgccg ggagcgccac gcttgcccgg cctacggata attcaccctc ttctgtttga
      901 agagggtaat gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc
      961 ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc
     1021 tttcggcttc ggttttttct cgccgcggaa ggcgctgtct ggctcaaagt tgaccttttt
     1081 gccgacctga cgccgcttgc cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg
     1141 cgccgtttta ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt
     1201 gcgctcgtcc atattaaccg cttcgacacg aacttccacg cgatcgccca ggcgataagt
     1261 ctggccgctg gattcaccca tcaggcgttg ccctacctgg tcaaagcgat agtagtcatt
     1321 gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa
     1381 gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat
     1441 gaagtcacac ttcagccagt cagacacatc gcgcgttgct tcatcggcac gacgttccgc
     1501 catcgaacag tgctgaccca gttgcagcat ctcttccatc gaataatggt agccgccggt
     1561 ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg
     1621 caacgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc
     1681 cagaccgaag tgaccacggt tttccggatc gtaaatcgcc tgtttcatcg agcgcaaaag
     1741 catggtttgc agcatttctg catcaggacg atcggcaacc gattccagta actctgcata
     1801 gtcgcgcggt tccggcttgt taccgcccgg caattccagc cccagctccg ccagcactga
     1861 acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc
     1921 tttggctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat
     1981 taacttgtgg gcgtcgttac gctgagtctg ttcgatacgt tcaatacggc gttcagcgtt
     2041 gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt
     2101 atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gggcgtactg
     2161 ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc
     2221 gtgagagctc atcaccgctt cgtagaattt gtagccagtc aggcggcctt tcgacgaaac
     2281 cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgagc acaggccgtt
     2341 agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtaccacg
     2401 attacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat
     2461 cgcgacccat aaacgccagc cgccgccgcg ttttttctca cagtaaactg catcgtcaaa
     2521 gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcagat cgacacggcc
     2581 cgcttttgct tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg
     2641 cggccagatg tacggaattt catgggtacg cagagcaata tcaaccgcca tgccggtgcc
     2701 catattatcg cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg
     2761 ttgagtcagt tcaaccacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg
     2821 gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc
     2881 ggtgaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc
     2941 ttcacgacga cctttacggt cagcgcccag cggctgagcc agcacctgat cgccatgaat
     3001 gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt caactcgcag
     3061 aaagccgtag ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg
     3121 cagcgcatag cactgacggc gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag
     3181 gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc
     3241 atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg
     3301 gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt
     3361 tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc
     3421 ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt
     3481 tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca
     3541 ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatt
     3601 gcactcgccg gtttacccaa gcgaatgccg ccattttttc cacgaacagc agtcacgtag
     3661 ccggcacgac taagttgatt gattattttg accatatgat tacgggagac gccgtagacg
     3721 tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc
     3781 gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg
     3841 gggaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc
     3901 agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac
     3961 cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag
     4021 aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag
     4081 atgatatcga tcggcacacc ggtcagctct tcaatgcgct tgatgtagtt cagtgccgcc
     4141 tgcggcaggc cactacgatc tttcacgccg aaggtggatt cagaccagcc cggcatggtt
     4201 tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttca
     4261 cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg
     4321 tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca
     4381 acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc
     4441 tgcttgcaga ggaactcgcc cgtttcatca aacagttcgg tcgggaacgg acctgcaccc
     4501 acacgagtgg agtaagcttt gaggataccc aaaacgtaat caacataacg cgggcccagg
     4561 ccggaaccgg tcgccacgcc accagcagta gtgttggaag aagttacgta cggataagtg
     4621 ccgtggtcga tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc
     4681 tgacgcgcct ggtcgagcag atcagaaacg tcaacaacca tagaagtcag aatgtcggca
     4741 acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta
     4801 accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg
     4861 tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg
     4921 ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc
     4981 agcgcaacgt gataatcaag gatcagcgga catgcttcgg acagcagcag acgctcacga
     5041 acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcagc cggagacagc
     5101 acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga
     5161 tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg
     5221 tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg
     5281 tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg
     5341 cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat
     5401 gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac
     5461 aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt
     5521 catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag
     5581 taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa
     5641 aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt
     5701 atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tgtccgggct
     5761 catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat
     5821 gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttctgc
     5881 atcgccttca ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac
     5941 ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg
     6001 acgtgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt
     6061 cggcaggttg atctgcttaa ttcgcacatc gacaacttca atacccagcg ccgccatact
     6121 gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc
     6181 ggcaatggcg ttatctgccg ccggggtagt aacttcatct tctgtacccg cagaaccgga
     6241 gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatgtcttt
     6301 cacgtccaga cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac
     6361 ttccgcttgc gaaatgtcgc caccgcccgt tgccaggtag taacggctga aatcgctgat
     6421 acgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc
     6481 ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg
     6541 tatcttgaaa tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac
     6601 cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta
     6661 aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc
     6721 tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga
     6781 ctggtggacg acgtgttgct tgctccactg gttgtggtgg aagaggctgg cggcagacgc
     6841 agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc
     6901 tggtctaatg gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg
     6961 ttgcccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg
     7021 gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg
     7081 gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc
     7141 ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc
     7201 gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag
     7261 ttgacgtcca gcagcgtgat acccatgtca tacggacgaa tcgtctcttc cagttcgcgc
     7321 tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta
     7381 ccgataaccc cgcgtagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc
     7441 acgctataca gatatttttc cggatcggtg acgcggtact gcacgttcat ctcaacgcgc
     7501 actacgttct cgtccgacgt cagcatcaca ccagaagctg ccagttcacg cacggcttcc
     7561 acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc
     7621 agatggctga atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag
     7681 aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacgcgaccg
     7741 ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg
     7801 ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc
     7861 ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt
     7921 ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc
     7981 catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct
     8041 tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg
     8101 acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca
     8161 ctctttttct attgcctgaa gctgataaaa acgacttctc aggcgccctt cctgcggcgg
     8221 cagacgcaat gtatgctgcg ccacctcgcc ggaaagacgc tccgtcaaag cctgaaaaag
     8281 ctgtggtatc cccgctccgg tctgcgcaga aagccagaca cggatcggtt tgttctcttc
     8341 atctctgtca atacgcggtt cgaaatcgtc cagcatatcg atcttgttca tcaccagcag
     8401 ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc
     8461 ttgtacacgc acatccgccg cgtcaatgac gtgcagcagc aatgtggctt gccgcgtctc
     8521 ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa taaaccctac
     8581 ggtatctgcc aacacggttt caccgacatc cgcaacgtca atacgccgca acgtcgggtc
     8641 gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag
     8701 agtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat
     8761 acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg
     8821 cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg
     8881 caaaccaatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc
     8941 cagatggcgc aactgcgcca actcaacctg caacttaccc tcatgggtac gcgcacgttg
     9001 ggcgaaaata tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc
     9061 caggttacgc tcctgcgccg ggctcagggc atggtcaaaa agaacgaccg aagctcccgt
     9121 cgcttttacg gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg
     9181 cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag
     9241 agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag
     9301 taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca
     9361 gcggggaacg caggatcgct ggctccccgt gtaaaaaaac agcccgaaac cttattcggt
     9421 ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat gatagttact
     9481 gctggtaccg ccaccggcgt tgttactgtg atgagaaacc gggcgagacg ggacaacagt
     9541 agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga tcacgaactg
     9601 atcaaaagac tcgatttgtc cttgcagctt aataccattc accaaataaa tagaaactgg
     9661 aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc ccttagccat
     9721 tctctctttt ccttatatgc ttatttgtac tttgaatctt tcgattctga aaaaattgcg
     9781 cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc tgtaatacgt
     9841 cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc caaccacgca
     9901 gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga taaaccattt
     9961 catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc acgcaacgaa
    10021 tggaaggcaa atccgtatgc aaatctcctc gggcaaaaag cgcccggact tctgcttcaa
    10081 aacctgaagc caacatctga tgaaaacgct gctcaattcg ttgatggagc agttcacggc
    10141 tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct gacgtttgcg
    10201 tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt
    10261 gtggatcatt tggatgaatc cttgctgccg caaccggatc gatctcctga agttgacgat
    10321 gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgt acttccgggt
    10381 ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac atcgtaccgc
    10441 ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc agcgcatcgc
    10501 ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatattcagc aatcggtgcg
    10561 gcgcggcgag taactcttca gcgttcggct tcgccgtccc gatatccatc cctttgtaaa
    10621 taagggcaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc tcaatggcta
    10681 acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct
    10741 tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac agattgtaac
    10801 agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc cgccagcagg
    10861 gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg tgcaatccac
    10921 tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat cagttcagga
    10981 atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc
    11041 tgtgcatcgg actggaaatc aatacccaat tccgccaggg cagactgcgc tttttctaat
    11101 gccgattttt cttcaccaga aacttttagc cgcaacggaa taagcagcgg ctgggcgcaa
    11161 actggcgctt cacccggcgt caattgtgcc tgacgcagcc aacgttctgc cactggcaag
    11221 gataaaagtg aaatgttgcc atcgcgctca agcaacgcac agtcggaatg gacgatagtc
    11281 agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg ttctggcgct
    11341 tttggttttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc accttgctgt
    11401 ttctggtaac ctggctgcgc attcggccag ggggcagccg gacgactgcc tgaggcgggc
    11461 gcaggagagt agcgcggagc taccggctca cgaactgccg gttcagcaaa gtgattgcgc
    11521 cccgccgcca cgcgattttc cggaatggca cgcggtgcag gttgtggttc atcgtccagc
    11581 ggtagcggcg tttccagttg ctgttgtagc acgctcagca cgccctgata gataaagtca
    11641 tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cagggtgcac gttgacgtcc
    11701 acctgatgcg ggtcgatctc cagatacagc acaaatgccg gttgttgatc ggcccccagt
    11761 ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc gatcgcgcat catgcgaccg
    11821 ttcacgtagc aatactgaat ttctgccagg gcgggcgtgg tgtgattcgg atcggccacc
    11881 cagccgcgca gggtgagatc gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa
    11941 gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg cactgcacgg
    12001 tactgacgca caattttacc gttatgcgac aggttgatcg tgacgtcgaa acgcgccagc
    12061 gcaatgcggc gaataatttc atcgatatgg ttaaattcgg ttttctcggt gcgcaggaat
    12121 ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct ccagcgtcgt tcccaccgga
    12181 tgcgccgccg gtttaaccgt cacgtccata tcgcgccctt cggcataggc ctgccaggct
    12241 tcctgttgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc
    12301 agcgcctcac cgcgaaagcc caggctgata atggcttcga gatcgtccag agaggcgatt
    12361 ttactggtgg catgacgagc cagcgccagc gccagctcat cttttttgat accgcagccg
    12421 ttatcacgaa tgcggataag tttcgccccg ccacgttcga tatcaatatc aatgcgcgtc
    12481 gcacctgcat cgaggctgtt ttccaccagt tctttgacta ccgacgcagg tcgctcgacc
    12541 acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat tggcatcaat
    12601 cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggccgt ttgtgccgtt
    12661 gcaccctgcg gcgcagattg catcggatgc gcaaggaaat aattgcgcag gcctttataa
    12721 atggcttctg ccagctgttg ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg
    12781 ttactgataa aaccggtttc gaccagtact gacggaatat ccggcgaacg cagaacgccg
    12841 agactcgcgt gttctggtcg gcgtttatgt atttcgccga tgcgttgcaa ctgactgatc
    12901 atactggtcg ccacatcata ccctacccgc tgggaatgac cgaactgtaa atccagcacc
    12961 gcctggctta aatacgggtc agactgactg ttcgccagca catcgcccgc tccgcccagc
    13021 agctccgact gtttctcgtg ctgctccagc cagctggcca tttcgctgtt ggcgcgacgg
    13081 ttagagagca cccatacgga agcgccagtc gcactgcggt tcggtgcggc atcagcgtga
    13141 atcgacacga ggaaattggc gttttgctta cgtgccacat cgctgcgccc catcaccgag
    13201 ataaagtaat caccgtcacg ggttaaaacg cccttaaaca tcggatcgtc attgagcaaa
    13261 gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc accggggccg
    13321 atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat tttatcgcca
    13381 gtgttagccg tcgcgcgcgc agccggtcgc gttaccgtat tactgctgat aacacccgta
    13441 gtgcggttac tttcagtttt aaacggattg cgcgctggtt cactgacgcg cggtgcgaca
    13501 accgcaggcg tttcaacgcg tttcgcaacc acaggcggcg gaggaggcgg tggcggcgca
    13561 tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat tctgccgctt caccgcttcg
    13621 gttttaccgt tttcggtaag atcgaccacc agccgtagcg tttgtgcatc tttaggcgtt
    13681 ccggagcgaa tcgccttcac cagattatta ccgctgaaca acaatggcag tccctgaatc
    13741 acgcccgttt gtttgatatc gagcgccacg gtgcgtttgc tttgatggct aaacgcataa
    13801 tcaggatcgc caataaaact caacgttatc cgcgcctgtt gattaccgtt agaaacctga
    13861 atatcagaga gcgtcgcggc acccacgtgc gcgcacagca gcagcaacgt cgctaccaac
    13921 caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc taaacgcgcc
    13981 agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc ttgtgcctga
    14041 taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg ttgtggccac
    14101 tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa ctccagctcc
    14161 tcgggatcgg caaggcggta caaatcaaag tgataaacca ttaaattgtc gagcgtatag
    14221 ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag agcctgtaaa
    14281 aagccccggc taaaggtggt tttacctgcg cctaaatcgc catacagata gattacggtt
    14341 gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttgcctg ctcatcaggg
    14401 agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa caatacgctg
    14461 tagcgtggaa aagagatcgg tcgccagcat tccgcgcgtt ccaaaacgcg ccgccagtac
    14521 gtcagctgcc gtaccgtgtg cgacacagcc cgcgcacgct gcatcatacg gcgacagttt
    14581 ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc cgctcgccat
    14641 gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga ccacggttcc
    14701 ggcacctttc aacaccgcta caccgccata acgttgtacc agacgttggg cgcaatgtaa
    14761 gcggtcactt tcaatttcag cgacggaaca gcctaacaac cgtgcggcct cgccaggatg
    14821 cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt tcaatgcatc
    14881 ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagggctt ttttccccca
    14941 ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca ggctttcggt
    15001 aagagagtcc atagtcagtt catgcaccat caattccggt cgtgcagtca gcagcggcgc
    15061 aatgttctca ctgcgggtca gtactcggac taaaccagca ccagcacgca gcgccgcttc
    15121 ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccaataatca ccagccgccc
    15181 gtgatcgcct ttatgcgaag tcgggcgacg cggtatcagc cactgagaaa gttgttctgc
    15241 cgaaaaccgc tgaattttcg tctcctgacc tgccagccag ctatccagcc ccagtgagtc
    15301 aaaatgcagt tgtccggtaa catcccgcgc ttttccagtg agcaagcctg gtttcagcgc
    15361 aataaaagtg atggtgtgat cggcgttgat taccgcgcct ggcgtagcgc cggtttcagc
    15421 cagaaggccg gaagggatat caaccgccac aatcggcgca gggtgagtat tagcgtggtc
    15481 gattaactgg ctaatggatt cgcggggcgc ttgctgcaag ccggtgccga gcagcgcatc
    15541 aacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct cgcctcccgc
    15601 gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt cactttcctg
    15661 ggccagcagc gtgacatcaa taccgatcgc tttggccagc cgcgcgacca cgtagccatc
    15721 gccgccgtta ttaccatgac cgcacagcac cagccagtgg cgggcgtcag gataagccga
    15781 acgacacacc tggaatgcgg cttcgccagc gcgaagcatc agctcataga gtgtgagccc
    15841 cagcgcatct gccgcctcgc gttctccgcg gcggatatcg tcggcgtacc agactgtgtg
    15901 tggtatactt acggggtttt tcttcattgt atggtccgtc atgtcagagc ccctcgatct
    15961 caatcaatta gcgcaaaata ttaaacagtg ggggctggaa ctgggctttc agcaggtagg
    16021 tattaccgat accgatctca gcgcgtccga gcccaaactg caagcatggc tggacaaaca
    16081 ataccacggc gaaatggact ggatggcgcg tcacggtatg ctacgtgccc gcccccatga
    16141 gttattgccc ggtacgctgc gcgtgatcag cgtgcggatg aattaccttc ctgctaacgc
    16201 cgcatttgcc agcacgctga aaaaccccaa actcggctat gttagccgtt atgcgctggg
    16261 ccgtgactat cacaaacttc tgcgcaatcg actcaaaaag ctgggcgaga tgattcagca
    16321 acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct gcgcctattc tcgagcgccc
    16381 gttagctgaa aaagcaggac ttggctggac aggtaagcac tcacttatcc tcaatcgcga
    16441 ggccggttcg ttcttctttt taggcgaatt gctggtcgat attccgctgc ccgtggatca
    16501 accagtcgag gaaggatgcg gtaaatgcgt ggcctgtatg acgatttgcc cgaccggtgc
    16561 catcgtcgag ccatataccg tcgatgctcg ccgctgtatc tcttatctca ccatcgaact
    16621 ggaaggggcg atcccggaag agttgcgacc gttaatggga aaccgtattt acggttgcga
    16681 tgactgccag cttatctgcc cgtggaatcg ctattcgcaa ctcactacag aagacgattt
    16741 cagcccgcgt aagccgctac acgcaccgga actcattgag ttattcgcct ggagtgaaga
    16801 gaagttttta aaagtcacgg aaggttcggc gattcgccgt ataggtcacc tgcgttggct
    16861 gcgtaatatc gccgtagcct taggcaatgc cccctgggat gaaacgattt tgacagcgct
    16921 ggaaagtcgt aaaggtgagc acccacttct tgatgagcac atagcgtggg cgattgcgca
    16981 gcaaatcgag aggcgaaatg cgtgcgtggt cgaagtgcaa ttaccgaaaa aacagcgtct
    17041 ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga ttcatccaca gcttgtgaat
    17101 aaaaataaaa acgcattgca attcaagccg gaaaaaatcg tcaagtgatc gatacgacaa
    17161 aacgaaatct taatttttaa cttatctatc aataggttaa aaaaatacta ttcaccgtgc
    17221 gaagtttctt tgaaattaag attacggcct gaggctgtgg ataactctgt ttacaagagt
    17281 ctgtcagaga catcaaaaac cagcaataac gcatcgcgtc gctgtggata ttttattgag
    17341 agaagaattt ggag
//
LOCUS       DAZKHK010000108        16982 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKHK010000108 DAZKHK010000000
VERSION     DAZKHK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.108"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..1465
                     /gene="cscA"
                     /locus_tag="ACHOS5_003738"
     CDS             32..1465
                     /gene="cscA"
                     /locus_tag="ACHOS5_003738"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP9778488.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDENDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQKNAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHEHLDGYRSIPLPQGDMLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWQLG"
     gene            complement(1473..2468)
                     /locus_tag="ACHOS5_003739"
     CDS             complement(1473..2468)
                     /locus_tag="ACHOS5_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9778489.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTLHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLESLLNAHFQSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRETRIPCPLLIRCST"
     gene            2698..2916
                     /locus_tag="ACHOS5_003740"
                     /pseudo
     CDS             2698..2916
                     /locus_tag="ACHOS5_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2934..4262
                     /gene="dsdA"
                     /locus_tag="ACHOS5_003741"
     CDS             2934..4262
                     /gene="dsdA"
                     /locus_tag="ACHOS5_003741"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP9778490.1"
                     /translation="MENAKMNSLIAQYPLVEDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAAAGGIIESELVAIPAMQKRLEKEYHQPIAGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GSQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4370..5908)
                     /gene="emrY"
                     /locus_tag="ACHOS5_003742"
     CDS             complement(4370..5908)
                     /gene="emrY"
                     /locus_tag="ACHOS5_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP9778491.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5908..7071)
                     /gene="emrK"
                     /locus_tag="ACHOS5_003743"
     CDS             complement(5908..7071)
                     /gene="emrK"
                     /locus_tag="ACHOS5_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP9778492.1"
                     /translation="MEQINSNKKHSDRRKYFALLAVVLFIAFSGAYAYWSMELKDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGIDMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7487..8101
                     /gene="evgA"
                     /locus_tag="ACHOS5_003744"
     CDS             7487..8101
                     /gene="evgA"
                     /locus_tag="ACHOS5_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP9778493.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8106..11699
                     /gene="evgS"
                     /locus_tag="ACHOS5_003745"
     CDS             8106..11699
                     /gene="evgS"
                     /locus_tag="ACHOS5_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP9778494.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEVFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTAHYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKVEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGFNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEITVFCQQND"
     gene            complement(11755..12900)
                     /gene="yfdE"
                     /locus_tag="ACHOS5_003746"
     CDS             complement(11755..12900)
                     /gene="yfdE"
                     /locus_tag="ACHOS5_003746"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP9778495.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSVLCQALELTELVNDPR
                     FSSNILRVQNHAILKEYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12974..13918)
                     /gene="yfdV"
                     /locus_tag="ACHOS5_003747"
     CDS             complement(12974..13918)
                     /gene="yfdV"
                     /locus_tag="ACHOS5_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP9778496.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13988..15682)
                     /gene="oxc"
                     /locus_tag="ACHOS5_003748"
     CDS             complement(13988..15682)
                     /gene="oxc"
                     /locus_tag="ACHOS5_003748"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP9778497.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALGAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15736..>16982)
                     /gene="frc"
                     /locus_tag="ACHOS5_003749"
     CDS             complement(15736..>16982)
                     /gene="frc"
                     /locus_tag="ACHOS5_003749"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP9778498.1"
                     /translation="TPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTR
                     HQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDH
                     MGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLV
                     SAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDK
                     LGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWE
                     NTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCA
                     PVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTA
                     AVLQELGYSDDEIAAMKQNHAI"
BASE COUNT         4858 a         3926 c         3697 g         4501 t
ORIGIN      
        1 ttaccaaatt tgcttaacca ggatgattaa aatgacgcaa tctcgattgc atgcggcgca
       61 aaacgcacta gcaaaacttc atgagcaccg gggtaacact ttctatcccc attttcacct
      121 cgcgcctcct gccgggtgga tgaacgatcc aaacggcctg atctggttta acgatcgtta
      181 tcacgcgttt tatcaacatc acccgatgag tgaacactgg gggccaatgc actggggaca
      241 tgccaccagc gacgatatga tccactggca gcatgagcct attgcgctag cgcctggaga
      301 cgagaatgac aaagacgggt gtttttcagg tagtgctgtc gatgacaatg gtgtcctctc
      361 acttatctac accggacacg tctggctcga tggtgcaggt aatgacgatg caattcgcga
      421 agtacaatgt ctggctacca gtcgggatgg tattcatttc gagaaacagg gtgtgatcct
      481 cactccacca gaaggaatca tgcacttccg cgatcctaaa gtgtggcgtg aagccgacac
      541 atggtggatg gtagtcgggg cgaaagaccc aggtaacacg gggcagatac tgctttatcg
      601 cggcagttca ttgcgtgaat ggactttcga tcgcgtactg gcccacgctg atgcgggtga
      661 aagctatatg tgggaatgtc cggacttttt cagccttggc gatcagcatt atctgatgtt
      721 ttccccgcag ggaatgaatg ccgagggata cagttatcga aatcgctttc aaagtggcgt
      781 aatacccgga atgtggtcgc caggacgact ttttgcacaa tccgggcatt ttactgaact
      841 tgataacgga catgactttt atgcaccaca aagcttttta gcgaaggatg gtcggcgtat
      901 tgttatcggc tggatggata tgtgggaatc gccaatgccc tcaaaacgtg aaggctgggc
      961 aggctgcatg acgctggcgc gcgagctatc agagagcaat ggcaaactcc tacaacgccc
     1021 ggtacatgaa gctgagtcgt tacgccagca gcatcaatct atctctcccc gcacaatcag
     1081 caataaatat gttttgcaga aaaacgcgca agcagttgag attcagttgc agtgggcgct
     1141 caagaacagt gatgccgaac attacggatt acaactcggc acaggaatgc ggctgtatat
     1201 tgataaccaa tctgagcggc ttgttttgtg gcggtattac ccacacgagc atttagacgg
     1261 ctaccgtagt attcccctcc cgcagggtga catgctcgcc ctaaggatat ttatcgatac
     1321 atcatccgtg gaagtattta ttaacgacgg ggaggcggta atgagtagcc gaatatatcc
     1381 gcagccagaa gaacgggaac tgtcgctcta tgcctcccac ggagtggctg tgctgcaaca
     1441 tggagcactc tggcaactgg gttaacataa tatcaggtgg aacaacggat caacagcggg
     1501 caagggatcc gcgtctctct tcccccttca cgaccttcaa taatatgcaa tgcagcttcc
     1561 cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg gcggtaaaaa cagatgcccg
     1621 acgccaacca gattatcaaa gcccattacg gcgacatcct gcgggatacg tacccccttc
     1681 gccagaagaa cctgataagc cacaaaggct gcgcgatcgt taccacatat cagaacatca
     1741 aaatctggtt tgccagattg gaagtgggca ttgagtaaac tttcgagatc ggtgtagtga
     1801 tcatcacctg ttgccatgtg aaattgtttc acctcagcca gatctcgtcc agcatcacgc
     1861 caggcctgct caaatccctg ccgacgatac cctgttgcca acgcactttc cggtagccag
     1921 aagcataacg gttgacgata gcccgccgcg agcaaatgca gtgttgattc atattgtgca
     1981 gtgtaatcat cagggatata actgggtaac gctgggtcat ccgccacaca gttcgccaat
     2041 acaatatttt caccatacag agactcaggc agcgtgatat gtcgcagccc cattgtagta
     2101 tagataatgc catccggacg gtgggcaagc agctgacgtg ccgcgcgggc agcgtcatct
     2161 tcagaaaaaa tattgattaa aaaactattc cagccgaact cgctggcggt ttgctcaatg
     2221 gcaagcagaa tatcaacaga gaaaggagtg gtagcagtgt cctgtgccag cacggcgaga
     2281 gtcgacggct tacgtccttg agcgcgcatt ttacgggcgg aaagatcagg aacataattc
     2341 agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag gacgcacaga ttctgcatta
     2401 tgcatcaccc gggagactgt catcatcgac actcccgcca ggcgagcgac atcctttaat
     2461 gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt agcagaaaca gacgtcactg
     2521 gcgagatcca acgatattct cgacgcccta tcacccgaca cggcaataca ataaaaagta
     2581 acaataattc ccggacaatt gtacccaatt tcgcctctgt tctcgccaac gagtaaccat
     2641 gtaggcaagt aggtaaaaca tgccaacctg gagaagaaga tataaatatg gatcattgtg
     2701 ctgccgctgt atcccgacat cagcccggaa attattgcaa ttgccatcgg ttcaggtgcg
     2761 attggctgca cgatcgttac ggactcgctt ttctggctgg tgaagcaata ttgcggcgct
     2821 acgctcaatg aaacatttaa atactatacg acagcgacat ttatcgcttc agtcatcgct
     2881 ctggcgggca cattcctgct gtcatttatc atctaagcgc aaagagacgt actatggaaa
     2941 acgctaaaat gaattcgctc atcgcccagt atccgttggt agaggatctg gttgctctta
     3001 aagaaaccac ctggtttaat cctggcacga cctcattggc agaaggttta ccttatgttg
     3061 gcctgaccga acaggatgtt caggacgccc atgcgcgcct gtctcgtttt gcgccgtatc
     3121 tggcaaaagc atttcctgaa acggctgccg cgggggggat tattgaatca gaactggttg
     3181 ctattcctgc tatgcaaaaa cggctggaaa aggaatatca ccagccgatc gccgggcaat
     3241 tgctactaaa aaaagacagc catttgccga tttccggctc cataaaagca cgtggcggga
     3301 tttatgaagt cctggcccat gctgaaaaac tggctctgga agcgggtttg ctgacacttg
     3361 aggatgatta cagcaaactg ctttccccgg agtttaaaca gttctttagc caatacagta
     3421 ttgctgtggg atcaaccgga aatctggggt tatcaattgg tattatgagc gcccgcatcg
     3481 gctttaaagt aacggtgcat atgtctgctg atgcccgcgc ctggaaaaaa gcgaaactgc
     3541 gcagtcatgg cgttactgtc gtggaatacg agcaagatta tggtgttgcc gtcgaggaag
     3601 gacgtaaagc cgcgcagtct gacccgaact gtttctttat tgatgacgaa aattcccgca
     3661 cgttgttcct tggatattcc gtcgcaggcc agcgtcttaa ggcgcaattt gctcagcaag
     3721 gtcgcatcgt cgatgctgat aaccctctgt ttgtctatct gccgtgtggt gttggcggtg
     3781 gtcctggtgg cgtcgcattc ggacttaaac tggcgtttgg cgatcatgtt cactgctttt
     3841 ttgccgaacc aacacactcc ccgtgtatgt tattaggcgt ccataccgga ttacacgatc
     3901 agatttctgt tcaggatatt ggcatcgaca accttaccgc tgccgatggc cttgcagttg
     3961 gtcgcgcatc gggctttgtc gggcgggcaa tggagcgtct gctggatggt ttctataccc
     4021 ttagcgatca aaccatgtat gacatgcttg gctggctggc gcaggaagaa ggtattcgtc
     4081 ttgaaccatc ggcactggcg ggtatggccg ggtctcagcg cgtgtgcgca tcagtaagtt
     4141 accaacagat gcacggtttc agcgccgaac aactgcgtaa tgccactcat ctggtgtggg
     4201 cgacgggagg tggaatggtg ccggaagaag agatgaatca atatctggca aaaggccgtt
     4261 aataacgttt caacgcagca tcgccatcct ttccctgggt gagcgatgct gccgatgacg
     4321 cagacttaag attctgccgt tttacctgct atagcctcct ctttttccat cacccaacgc
     4381 ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa
     4441 tagccgccat acggaaaatc tcatttgcag aaatagaaag tgactgctga gttatttcat
     4501 tattaatttc attaagaact cctgaaagcg aaccatagta tttatccata atttgtgacg
     4561 atgaattaaa cacggggtta aattgatcga tggttgctgt caactgacta tggtgtaacg
     4621 attcgcggcg tccccacagt gtcattgtca acgacgtacc aactgatcct gacaaggtgc
     4681 gaaaaaaatt actcatactc gaggcattgg caaatttatt atctggcaag cctgaaaacg
     4741 aaatcgttgt taagggtaaa aagaaacagg caacggcgaa tccctgaaaa aactgtggca
     4801 aaatgatgcc tgtaaaatca atcgttggca taaatgtcac agaacgccag taatagcaaa
     4861 ccgcatacat caaaaaacta aatgtcacca acaaccgcat atcaattttg ttgccataac
     4921 gtcctatcaa aggtgatatt aatagcggca tgataccgat gggcgcatag gcaagtccgg
     4981 cccatatcgc attatacccc atcgtttcct ggagtaactg cggcataaga actatcgctc
     5041 cagagtaaaa taaatacgcg catgtgatac tcacaatacc aatggtgaaa ttacgggact
     5101 taaacaaact gaggtcaaga atcggattct ctgaggtcga ctcccaaatg actaaagaga
     5161 tcagagaaat aactgatact actgttagta gtattattgt actcgagttg aaccaatcca
     5221 gatcgcgccc tttatcaagc ataatttgca agccaccaac accgagcact aacagggtca
     5281 gtcctggtag attcattttg accggtgaag tctcagtttc tcttccttta agtaaggtta
     5341 agcataatgt caggacgata atccccatag ggacattgat taaaaatatc caaccccagc
     5401 taaagttatc gcaaatatag ccgcccaata tcggcccaca tatcggagcg ataatcacgg
     5461 tcattgacca taatgccaga gcaaatgttc ttttttctgg tggataattc cttaataata
     5521 aactctgtga cagtggaatt aacggccccg ccattaaccc ctgaacgact ctaaaaaata
     5581 tcagcacatc aagattggtc gataaactac acattaatga agacagcgaa aaaaaggtta
     5641 ctgaaagtaa aaataacctt aattcgccta ttctttgtgc cagcctgccg gtaacaggga
     5701 tcgcaatggc atttgctacg ccaaacgagg tgataaccca ggtgccttcg tctgttgatg
     5761 ctcccagaaa gccagatatt gtcggtattg cgacgttaga aatagtggaa tccaacattt
     5821 gcataaacgt cgctaatgac aatgcaatag tgacgcacca taacgtccca ccggttaatg
     5881 gtgcaggagt tgatttagtg attgccatta aagttgtcca ttatgcgaaa taatgttgct
     5941 aatttctttt tctatcggac tggtatcgat aactaaagct ttactggtat aagcaggcat
     6001 ggacgtcacg gttgaagcca gatcgggcat ctcggcaatg tcttcattct tcgtatcaat
     6061 agttgcagtc atcgataaac caatgcgcaa agggtgttcc atgagttctt ttggatccag
     6121 agaaacttca accggtacac gctgaacgat tttgatccag ttccctgtcg cattttgtgc
     6181 aggtaataag gagaacgcat tgccggttcc catatcgatc cctgtcaccc gaccatgaaa
     6241 cacaacattt tcaccataaa gatcgctgat aatattgacc gattgaccaa tccgtacatc
     6301 cgtgagttgt gtttctttaa agttggcatt aacccacatt tgacgcgccg gtacgacagc
     6361 cattaatgat tgtccgggac tcactgtttc gccgacctga acacttctct gggcaatata
     6421 accggtaacc ggactcttaa tatccgtacg tttaagcgcc aaccaggctt ctttggttgc
     6481 atctgcagct tcaatgactt gtggctgacg gtttaatggt gtgttcatta ctaaagcttt
     6541 attcgcttta taagcctgga tagcagcatt caatgccgct ttgctactta ttaacgtatc
     6601 tttggtatgc tccagcgctt cttttgaaat aaccccttgc ttcgctaaag gcacacgacg
     6661 gttataatct tctaaagatt gttgatactg aatacgtgct gaagcgactt cggcactgta
     6721 ttgtttatcc tgtaagtata gtttattcgt ttgccgaaca atatttgcca gattattttt
     6781 agctttattg agtgcgatag tggcatcagt tttatccagt gaaactaaaa tgtcaccttg
     6841 tcgaacgtag ttcgtatctt tatgattaac gacagtgacg ctgcctgaga cttgtgcaga
     6901 aattggatct gcatttcccg tgacataggc gtcatctgta ctaatcatgt cttttaattc
     6961 cattgaccaa taggcatagg cacctgaaaa cgcaataaat aaaactaccg ctaacaaagc
     7021 aaaatatttt cttctgtcag aatgtttttt atttgaatta atctgttcca ctattatctc
     7081 tcatttcgca tagatgaaat ttatgaattg aatgaaaaga gtattcattc ctcatatcaa
     7141 acacagtgca tgttttgcac attatcccgg gaacgttgga gggctaattt aaatgaagtg
     7201 caaaaataac aacagaggac taaaccgcgg cttttgcaat acaattctta cgcctgtagg
     7261 attagtgaga aaacttatag tgctcatttg aaactataaa tcatcggtat catccctgat
     7321 tttattgttg acattttatt tatgccgact atttatatgg tatacttgtc gaattatctt
     7381 aaaggaagct caaattttct tatttttatt gagaaaatga gatgatacct tatgtctgta
     7441 ttactacagg gagaagggaa atgcttcatt gcaaagggaa taatctatga acgcaataat
     7501 tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat
     7561 tgaaatctta gcagagttga ctgaaggcgg aagcgccgtt cagcgggtgg aaacacttaa
     7621 gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga
     7681 aacgctgaga aagcgccaat atagcggaat tattattatt gtctccgcta aaaatgacca
     7741 tttttacggg aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg
     7801 catgaacaat atcattgcag ctattgaagc tgcaaaaaat ggctactgct atttcccctt
     7861 ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc
     7921 gaaacaagaa attagtgtta tgcggtatat tcttgatggc aaggataata atgacattgc
     7981 tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgct tgatggaaaa
     8041 attagaatgc aaatcactga tggatcttta cacattcgca cagcgtaaca aaatcggcta
     8101 accacatgaa gtttttaccc tatatttttc ttctctgttg tggtctttgg tcgaccataa
     8161 gtttcgcaga cggagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac
     8221 ttgatccact acgtctgagc aacaaggaat tacgttggct cgcgagcaaa aaaaatcttg
     8281 tgattgcagt acacaagtcc caaacagcta cgttgttgca taccgattcg cagcaacgga
     8341 ttcgtggtat taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa
     8401 cactccggga atacgcagat catcaaaaag caatggacgc gctggaagac ggtgaagttg
     8461 atatagtgtt atcacattta gttgcttcgc cgcctcttaa tgatgacatt gctgcaacca
     8521 atcctctgat aattaccttt ccggcgctgg tcactaccct tcacgattca atgcgaccgc
     8581 ttacctcatc aaaaccagta aatattgctc gagtagcaaa ttatccccca gacgaggtaa
     8641 ttcatcaatc atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag
     8701 catccgtctc agccggacag aatgattact ttattggtag taacatcatt accagcagta
     8761 tgatttcccg ttattttact cactccttaa atgtagtgaa gtattataac tcaccgcgtc
     8821 aatataactt tttattaacc agaaaagatt caatcgttct taatgaagta ctcaatcgat
     8881 ttgttgatgc tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag
     8941 gaaacctggc ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta
     9001 agcagcatcc cgatttaaag gtgctggaaa atccttactc gccaccctat tctatgacag
     9061 atgaaactgg ctcagttcgg ggcgtgatgg gtgatattct taatattatt accttgcaaa
     9121 caggtttaaa tttttctccg atcaccgttt cacacaatat tcatgctggg acacagctta
     9181 accccggtgg atgggatata ttgcccgccg ctatttatag tgaagatcga gaaaataatg
     9241 tttcatttgc tgaagtcttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg
     9301 acagtgaaca aacattaaaa aaaggaatga aagttgccat tccatattat tatgagcttc
     9361 attcgcaatt aaaagagatg tatccggagg tagagtggat aaaagtcgat aacgccagcg
     9421 ctgcatttca caaggtcaag gaaggtgaac ttgatgctct ggtcgcgaca cagttaaatt
     9481 cgcgttacat gatcgaccat tactatccta atgaacttta tcattttctt attcccggcg
     9541 ttcagaatgc atcactttcg tttgcttttc ctcgcggaga accggaactt aaggatatta
     9601 ttaataaagc actgaatgca attcccccaa gcgaagttct gcgcctgacc gaaaaatgga
     9661 ttaaaatgcc caatgtgacc attgacacat gggacctcta tagcgagcaa ttttatattg
     9721 ttacgacatt atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac
     9781 gctcagttcg tcgtcgtaaa gttattcagg gtgatttaga aaaccaaata tcattccgga
     9841 aagcgctctc ggattcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca
     9901 ttagtcacaa tagtgcattt gaacattatt tcactgctca ttactataaa aatgcaatgt
     9961 taccattaga aaacagtgaa tcccccttta aagatgtttt ttctaatacg catgaagtca
    10021 cagcagaaac gaaagaaaac cgaacaatat acacacaggt ttttgaaatt gataatggca
    10081 tcgagaaaag atgcattaat cactggcata ccttatgtaa tctgccagca agcgaacatg
    10141 ctgtttatat ttgtggttgg caagatatta ccgagacgcg tgatttaatt catgcactcg
    10201 aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa aagccagttt ctggcaacaa
    10261 tgagtcacga aataagaaca ccaataagct ccattatggg cttcctggaa cttctgtcgg
    10321 gttctggtct tagcaaggaa caacgggtgg aggcgatttc acttgcctac gccaccggac
    10381 aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact
    10441 atcaacttca accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt
    10501 tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg cagcagtacg cttcctgaac
    10561 gctacctggt taagatcgac cctcaggcgt ttaagcaggt cttatctaat ttactgagta
    10621 atgctctcaa atttactacc gagggggcag taaaaattac gacctccctg ggtcacattg
    10681 atgacaacca cgctgttatc aaaatgacga ttatggattc tggaagtgga ttatcgcagg
    10741 aagaacaaca acaactgttt aaacgctata gccaaacaag tgcaggtcgt cagcaaacag
    10801 gttctggttt aggcttaatg atctgcaaag aattaattaa aaacatgcag ggcgatttgt
    10861 cattagaaag tcatccaggc ataggaacga tatttacgat cacaatcccg gtagaaatta
    10921 cccaacaagt ggcggctgtc gaggcaaaag tagaacaacc tataacacta cctgaaaagt
    10981 tgagcatatt aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa
    11041 atctattagg atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaagtca
    11101 gtatgcaaca ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg
    11161 agttgactcg caaactccgt gagcaaaatt cttccttacc catctggggg cttacagcca
    11221 acgcacaggc taacgaacgt gaaaaagggt ttaattgcgg catgaactta tgtttgttca
    11281 aaccgttgac tctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata
    11341 ttgcacctca gtatcgccac cttgatatcg aggccctgaa gaataatacg gcgaatgatc
    11401 tacaactgat gcaggagatt ctcatgactt tccagcatga aacgcataaa gatctacccg
    11461 ctgcgtttca tgcactagaa gctggcgata acagaacttt ccatcagtgt attcatcgca
    11521 tccacggtgc ggctaacatc ctgaatttgc aaaagttgat taatattagc caccagttag
    11581 aaataacacc tgtttcagat gacagtaagc ctgaaattct tcagttgctg aactctgtaa
    11641 aagaacacat tgcagagctg gaccaggaga ttactgtttt ctgtcagcaa aatgactaaa
    11701 tagcggctcc cacaatgttc aaatgtggga gctatttacc agcacatctt acatttatga
    11761 tgagaactcc tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac
    11821 atgcgggtcc gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcccccggc
    11881 ttcaatcaac atatttctcg cctgagtttg tggtaaatta atggcctcag ccacacttaa
    11941 taacggcgcg acgggcacac caacttcatg tattctggct aaccaaactt cagctgcctg
    12001 cgtttttaac gttcgctcaa tatattcttt aagaatagca tggttttgta cgcgtaaaat
    12061 attgctgcta aatcggggat cattaaccag ttctgtaagc tccagtgcct ggcataacac
    12121 agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac
    12181 atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt tgtggtgact tcccagtcgc
    12241 gatatatgcc atcagtccat gctccagaaa actcagagtg gcatcaaaca tcgctatatc
    12301 gacatgcgcc cctctctggc tcttttcgcg gccataaagt gcactcacta ttccactgaa
    12361 taaataaaca ccgccacaga gatccgcaag agaggtacca acgcgcactg gcggagcatc
    12421 agggtatcct gtttccatca ttatcccgct cattgcctga atgatggtat cgtaggcagg
    12481 agcatctttt agcggaccgg tatgtccgaa acctgacgat gaagcatata taaggcgcgg
    12541 gttgatttct tgtaaccttt cccatgaaaa cccgagtttt tccattgtac ctgggcgaaa
    12601 attctcagct aatacatcag cttgtttaag catatttata aatatacttt tatcgtgatc
    12661 attctttaaa tcaagaacca cactctcttt gccatgatta ataaaactgt aatagagtga
    12721 ctgtccatcc acatagggac caaatgtgcg ggtatcatca ccatgacccg gcggctcaac
    12781 tttaattacc cttgcaccca tattacaaag aagttgagtt ccgaaaggcc cattaaggac
    12841 atgcgtcata tcgataacta ataagccttc aaacggccct ttgcattcat tatttgtcat
    12901 ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact tcggcattta tgtaaaatca
    12961 ataacatgat taattatgaa accagtcgac tgacataaat ccacaaagga gccgtgacga
    13021 caaaacccag tacgctcaca gccaatgacg ctgtaccagt gcgggtgtag acattaaacc
    13081 ggctggcaat aatgattccg gagaatgccg gcggtaatgc gcctgccagt accatcatct
    13141 gcaaatgttc gctattcaaa tgacatgcca taccgacgag aagcagcgcc agtggcatca
    13201 gaatcagctt caggaaagtg ttataagcaa tttcggcact gaactcgaat ttatgtgcag
    13261 ccagagtcaa ccccgcagcg aataccgcta cccctgagtt agccttcgca atcagattaa
    13321 aggttgggtc ccatgctgcc ggaattttta ccccaaccaa tactaggatc gttgccagaa
    13381 caggagccca tactactggc tcctttgccg cagaaattaa tgcgctcaga ttactattct
    13441 tcttaccatc cgctcctgag gaaggattca gcaaatacag accaatagga atagtaattg
    13501 cgttgacaat aatagaaata attgctacca ctaaacctgt tgatacggaa tcaccataaa
    13561 taggatcgag aactgcaaac cccaggaatc caatggtagg tgaacctgca attaatgcac
    13621 atactgctgc ttctgcatgg gtacgtttaa aaaatttgta gcaaccgaac caggagaaaa
    13681 agaaacatcc gacaataacc acaagtgata caagggtcag gcgagtgtcc gcaaaaatca
    13741 tttccctgtt tgccctggta atagaaacaa atagagccgc aggaagagcg tagtttaata
    13801 ccagtttatt aaatgcccgg gcttgatctt ctgaaaaagt ttctcgtctg ccgctaaaat
    13861 aacccaacag catgattacg ataataggca ataaatcgcc aataaaaaat gttagcataa
    13921 tagtatttcc gataagggtc ccctcaccat cgcataataa gttggcaaga ggagacgatg
    13981 tcagtattta attaccagcg acttgttttg ggttaagttt ggtaatatgg ccactttcag
    14041 ttcctgctgc tgggtcgatg accacattaa taatggtcgg tttgcgcgac tgaattccgg
    14101 tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt atagccaacg ccacgaaacg
    14161 catccattaa tttgtcatac cttgcatggt gcaacagatc cgttggcgat ggtgcaccag
    14221 cgccgctgag atcaacaccg tctcctctgt agatgccgcc attattaaaa ataacgatcg
    14281 tcaccggcag gttatatcga caaatcgttt caatctccat cccactgaaa ccaaaagcac
    14341 tatcaccttc aatggcgaca accggagaac cagaggtcac gctagcaccg atggcatagc
    14401 ccataccgat gcccatgaca ccccaggtgc cacaatccag acgacgacgt ggtttataca
    14461 tatcaataat atttcgtgca ttatccaggg tatttgcccc ttcattaact aaataaatat
    14521 cctggttctc gcgcaaaaca tcgcgcacgg cacccaatgc attaaagtaa tttaatggtt
    14581 gggtatctgt acttaacttt tcatgcattt tttgtgcatt ttgctgcttg tggatattta
    14641 aaatatcgcg ccacaccagt ggagtcgtga atgtgttttg tttcaattct gccagcatac
    14701 cttgcatact ggatgcaata tcgccaacga ctggcacagc aatggggcgg ttgctgtcaa
    14761 tttcctgcgg ttcaatatcc agttgaataa actgtgtatc tgccgcccat ccttttttac
    14821 cgtgtgccag taaccaattc agtcgtgcac caacaagcat gacaacgtca gcatttgcca
    14881 gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt atcttcgagg atccctttcg
    14941 ccatagacat tggcaggaat ggaatctggg cactttcaat aaattcacga agctgttcat
    15001 cagattgtga atacgccgcg cctttgccaa ggataattaa tggtcgttca gcttttgcta
    15061 aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa taatgctggc gacggatttt
    15121 caactttaac aatcgtggtt aacgcttcgt ctttttccat cgtcgcggcc aggacatttg
    15181 ctggcaaatc aagataaaca ccgccaggac gacccgatac tgagacccgg atagcgcgtg
    15241 ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa tgctgctttg gcatacggtt
    15301 ttgccgcatt catttggtcc agctcttcat aatcaccttg ctggagatcg acgatcgcgc
    15361 ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt taccgttgca ttggccaatg
    15421 cggtcaaacc gttgaggaat cctggcgcag acactgtcag gcagatccct ggtttttggg
    15481 taagaaaacc gcttgctgca gcggcatagc ctgccgactg ctcatgacga aaaccaatat
    15541 aacgaatgcc ttccgcctgg gcatggcgcg ccatatccgt cacaggaata cctacaacac
    15601 cataaatagt gtcaatatta ttctgtttta atgcttcaac gatgatatgc ataccatctg
    15661 tcatttgaag ttgatctgac atgattaata acctcgaaat ttttataaat tgggagccag
    15721 aagcccccgt taatatcaga tggcgtggtt ttgcttcatt gcagcaattt catcgtcgct
    15781 ataacccagc tcctgcaata cagcagcggt atgttcacct aatagcggcg cagctttaat
    15841 atccggcgta aaggcagaga atttcattgg acaaccaact gtcagatatt ttccacgcaa
    15901 cggttgttcc acttcgacaa cactgccact ttggcgcaaa gagggatcaa gtgaaatttc
    15961 tttcatactt aaaaccggtg cacaaggaat atcaaactga gtcaaatagg ccaccgcttc
    16021 atgtttatca atagtgacag tgtatttttc gatttcagca aaaatatcga aaatatgtgg
    16081 ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc cattctggtt tgccgatggc
    16141 tttacaggtg ttttcccagt tttgctcctg aatagtgaaa taaatatagg cgttaggatc
    16201 ggtttcccag cctttacatt tcaggatcca gccaggctga ccgccaccac ccgcattacc
    16261 accgcgggga actgcatcac caaatgtgcc attcggatac tgcgggtatt cttccagata
    16321 ccccaactta tcgagacgct gctggtcgcg taatttcacg cggcaaaggt tcaacacggc
    16381 atcctgcatt gacatggtga ctcgttgccc acgccccgtt ttttcgcgat gcagcaaagc
    16441 agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg tcacccaacg ctgcagcgct
    16501 taccagcggc ggaccgtccc aaaaacctgt agtagatgcc gcgccacccg ctgcctgagc
    16561 aacgttttca taggctttta cattcacata aggcgaacac tcatcaaacc ctttgatcga
    16621 accaaaaatc agacgtggat tgatttcttg aatatgctcc caggtgaagc ccatatgatc
    16681 aatggcccct ggatgaaagt tctcgactaa gatatcagct tcgcggatca gcttttccat
    16741 tacctctttg ccttccgctg ttttggtatt taattcaata gaacgtttgt tactgttaag
    16801 catggtgaag taaagcgcat cgatatcagg aatatctcgc agctggtggc gcgttacgtc
    16861 accaacgccg ggacgttcaa ttttaatgac gtcagcgcca aaccaggcca gcatttgagt
    16921 acaagatggg ccagattgca caccggtgaa atcgagaact ttaattcctt gaagtggagt
    16981 tg
//
LOCUS       DAZKHK010000109        16947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKHK010000109 DAZKHK010000000
VERSION     DAZKHK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.109"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..473
                     /locus_tag="ACHOS5_003750"
     CDS             132..473
                     /locus_tag="ACHOS5_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFP9778499.1"
                     /translation="MFSFPASPCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            473..715
                     /locus_tag="ACHOS5_003751"
     CDS             473..715
                     /locus_tag="ACHOS5_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778500.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            792..1106
                     /locus_tag="ACHOS5_003752"
     CDS             792..1106
                     /locus_tag="ACHOS5_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP9778501.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            1119..1481
                     /locus_tag="ACHOS5_003753"
     CDS             1119..1481
                     /locus_tag="ACHOS5_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP9778502.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            1478..2140
                     /locus_tag="ACHOS5_003754"
     CDS             1478..2140
                     /locus_tag="ACHOS5_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778503.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            2140..3048
                     /locus_tag="ACHOS5_003755"
     CDS             2140..3048
                     /locus_tag="ACHOS5_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778504.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            3038..4525
                     /locus_tag="ACHOS5_003756"
     CDS             3038..4525
                     /locus_tag="ACHOS5_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778505.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            4561..4953
                     /locus_tag="ACHOS5_003757"
     CDS             4561..4953
                     /locus_tag="ACHOS5_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP9778506.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            5091..7787
                     /locus_tag="ACHOS5_003758"
     CDS             5091..7787
                     /locus_tag="ACHOS5_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFP9778507.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDSMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            7792..8169
                     /locus_tag="ACHOS5_003759"
     CDS             7792..8169
                     /locus_tag="ACHOS5_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9778508.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            8547..8735
                     /locus_tag="ACHOS5_003760"
     CDS             8547..8735
                     /locus_tag="ACHOS5_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9778509.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQAECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            9116..9394
                     /locus_tag="ACHOS5_003761"
     CDS             9116..9394
                     /locus_tag="ACHOS5_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778510.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
     gene            9586..10218
                     /locus_tag="ACHOS5_003762"
     CDS             9586..10218
                     /locus_tag="ACHOS5_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9778511.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            10354..10749
                     /locus_tag="ACHOS5_003763"
     CDS             10354..10749
                     /locus_tag="ACHOS5_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778512.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            10746..11720
                     /locus_tag="ACHOS5_003764"
     CDS             10746..11720
                     /locus_tag="ACHOS5_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778513.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            11734..13155
                     /locus_tag="ACHOS5_003765"
     CDS             11734..13155
                     /locus_tag="ACHOS5_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP9778514.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            13160..13501
                     /locus_tag="ACHOS5_003766"
     CDS             13160..13501
                     /locus_tag="ACHOS5_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778515.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            13508..15004
                     /locus_tag="ACHOS5_003767"
     CDS             13508..15004
                     /locus_tag="ACHOS5_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP9778516.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
     gene            complement(15055..15294)
                     /locus_tag="ACHOS5_003768"
     CDS             complement(15055..15294)
                     /locus_tag="ACHOS5_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778517.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            15484..15747
                     /locus_tag="ACHOS5_003769"
     CDS             15484..15747
                     /locus_tag="ACHOS5_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9778518.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            complement(15793..15968)
                     /locus_tag="ACHOS5_003770"
                     /pseudo
     CDS             complement(15793..15968)
                     /locus_tag="ACHOS5_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            16160..16732
                     /locus_tag="ACHOS5_003771"
     CDS             16160..16732
                     /locus_tag="ACHOS5_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778519.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
BASE COUNT         3892 a         3791 c         5319 g         3945 t
ORIGIN      
        1 gttgtttgcc ttcatccaca ccagtgtatt ctcccgtaaa aattttctgt tttgcaaata
       61 gagtttatcg ctgatccagt cctctgcctt acgcatcctg tacgcactgt ttaactctga
      121 cggaggtgcg tatgttttct tttccggcat caccctgccg tgccggccgt ctggccggtg
      181 ccgcggggat gctgtttctt ttgtcctttg cattacctgc gcaggctgcc gaaaaggatg
      241 aactggccac tgtaatgcgc cagcttgacc acgtgcaggc agcgctggag cgggccagaa
      301 cggcctctct gcaggcttct tcacaggggg aacgctactt ttttgattat ctgcgtgccc
      361 gtgcagatct gcagaccatt cgtgacggta ttgaccatta tctggcccct tcccgggcgc
      421 aaccccggtc ttccgcagcc gtgaccggtg actaccggcg ggagcgtccc tgatggcgat
      481 gacggccctg cagaatacgg ccttccgggc tgcttcaggt gaagtggatc tcagtgtgct
      541 gcatctcatc acgctgggat ttctgctggc agtcgtgttc ctctgggcag cgtggagtct
      601 gacggatgcc tgggcaggct ggcgtagcgg tgatgtccgg gatggtgtgt tcggctggtt
      661 tatgttccga ctcgtcctgc tgctgatctg cagtatctgg atgtttgcgg gttgatttca
      721 gtgtcttgtt taagtttcag gaggaaataa tgcataaaaa aatgcgcatc tgtcgccagg
      781 cctggtatgc catggcggtg tttctgtgta ccgggatcac acctgctctg gctgatttgc
      841 ctcagataga gccgccaaca tccggtggtg gtggtggact tttcggacag atcaaaggct
      901 atctccagga tggtgttgtg ctgtttggtc ttgtgctcag tgcagttgct tttgtgaagg
      961 tggcggaagc ggcgctttcc accttctcag aggttcgtga cgggaaggcc ggatgggggc
     1021 agtttggtgc catcgtggtg gttggtgttg ttctgctggt cgccattatc tggctgctgg
     1081 gcaagtcggc ctccatcatt ctgtaaggag acatccccat gcagaccatt cgttttctgc
     1141 ccgatcgtct gaacgtggag cctgcggttt ttcgtggttt taccacaccg gaactggggc
     1201 tggcggccct gacaggcgct gtggcagggg ggatctggcc gttaccgctg attccgctac
     1261 ttggctgggt gattatcccg accgggatgt tgctgactcc cctgctgctg gtctggttcg
     1321 gtggcgtctg gatcagccgg ctgaagcggg gaaaacctga caactggctc tggcagcatc
     1381 tggcgaagaa aagcagtgca tggggtggtg gccggaaaac atttatccgg gaggcgcatg
     1441 gctggtccct gcgccgtacc cgtagcaggg ggaaaaaatg agtcgttttc gtcatgctct
     1501 gcgggatcgc gaccagcata tcctgacact gcgtattttc agcggtgtgc tggccggaat
     1561 tctgatgctg gtctgtatcg ggtggatgac ggcaccggac agactgacaa ttcacaatcc
     1621 gccggatctg cgttccggca gtacccgtcc ctggtgggaa gtcccgccgt caacggttta
     1681 ttcctttgcc ttttatatct ttcagcaact gaatgcctgg ccgaagaacg gagaggtgga
     1741 ctacccggcg aaaattcatg cgctctctgc ctacctgacg ccatcctgtc gggagattct
     1801 ggagagtgat gcccgccgtc gcagtgatgc gggggagctg ctggagcgtg tgcgcgtggt
     1861 gtatgaaatt ccggaacatg gctacggggg caggtacaac aaaaatccgg tgacggtgga
     1921 agggcgggat aactggcagg tacagctgga tctggtggca gatgagtatt ttcatgctga
     1981 gccggtaaaa cgggcacttg tccgttatcc gataaaggtg gtgcgcatgg agggggatcc
     2041 tgaacgtaat ccgttcgggc tggcgctgga ctgctacagc agtacaccac agcggctgga
     2101 agcagcctcc ccggcagccg ggaaggggga taatcagtga tgaacggagc tgaacgttgt
     2161 ttataccgta gtattgtgat ggtcctgtca ctgctggttt taccactgag cgcacgggct
     2221 gatgagctga tgaaatggga gcgcattccc ttacccgtta ccctgatggc aggacaggag
     2281 cgggttattt ttgcagataa aaatgtgcgg gtgggcttcc cgccagcact gaacggaaag
     2341 ttgagggtac agagtaccgg tggggcagtc tatctgaaag cagagagtgt ttttccgcag
     2401 acgcgtctgc aactgcagga tgtggagagc ggagaaatta ttctgctgga tgtgacagca
     2461 gcagaaaaag ggcccacaga gcccgtacgg attgtgtatg agggaagcgt cagtacggtc
     2521 agtcaggctg gcacagcggc gtctgcacgc tctgcagaac acaaaaaggt ggtacccggc
     2581 ggggatgacg gctcgcaggc cctccgtcag ccggtgaagt atgcagagcc tttacccgtt
     2641 ctgttgaccc gctatgcggc acagtcgctg tacgcaccgt tacggacgct ggaagcggta
     2701 ccgggtatcc ggccggttaa tacacatctg cccgggaaaa tcaccacact ctatccgtca
     2761 gagccactga cagtcacgcc actggcagcc tgggctgcgg gtgggcgtgc agttgttgca
     2821 ctgaaactga cgagtacggt aagccgcaaa attatccttg atccgcgggc acttcaggga
     2881 aaattcattg ccgcgacttt ccagcaccgc tggctgggtg cagcaggtac ccctgaggat
     2941 accaccacgc tctatgtggt gacggcagaa cggccggaac aggcgtttat cgcggagcct
     3001 gcctcccgtc gcattccttc acggggggag gtgaagcatg cagattaaat ccaatacgat
     3061 cgtgaagtat gtggtgccct gtgtggtggt ggcggggatc ctgacgggat taaaaagctg
     3121 tggaggagat accggggagc cctcgtctgc ggaaagctca ggaacgctgg ccggactgtc
     3181 accggatgaa ctgaaggccc tgggcgtgga aggagacaca cctgaagata ccctgcgcac
     3241 actgattggg cgtatgaatc aggtgaggga gcggcaactg gtactggaca aacagaatga
     3301 aaagttgctg aaagaaaatg aaatgctgcg caagcggggc acggatgttt ccggtcaggt
     3361 cagtgaggca gtggcaggat tgcagaaaaa ttatgagcag tcgcagcgcc agttccggaa
     3421 tgaacagaac gcgttaatga cacaggttca gtcactgaca gagcaactga agcgtcagca
     3481 ggagagtgct gatgccggcg ccggaggaca ggagagcgat attccagcag gtctgggcct
     3541 tgacggactg gggagcgtga caggatcggc agcctcaccg ggaacggatg gtctgatgtg
     3601 ggtgacaccg gccgatcagg tgcaggatga agcgtcgtcg gtgggggata agagcggacc
     3661 gcgttttcca gtctcttttc tggatgacaa tccactgacc cgtcagaaag cggcttatga
     3721 acagcaggtg aagggataca gcaatgaaca gcagaaggcg agcacccggg cagtgtatac
     3781 cctgccggag aactcgacgc tggcaggcag ccgggcgatg acagcgcttc tgggacgggt
     3841 tccggtgaac ggaaccgtga cggatcccta tccgttcaag atcctggtgg gtaaggataa
     3901 cctgacggca aacggaatag agctgccgga tgtggaaggt gcagtggtgt ccggtactgc
     3961 gagtggtgac tggacgctct cctgtgtccg tgggcaggtg aacagcatca cgtttgtttt
     4021 tgccgacggg acagtgcgta cgctgccgtc atcagacgtg aacggcagca gtaacgcaca
     4081 ggcaaaaaat aacagcagta caggaggcat tggctggatt tcggatgatg acgggatccc
     4141 gtgtatcagc ggaacccgta aatccaatgc ctccacgtat ctgccgacaa tatttgccct
     4201 gtctgcagcc ggcgctgcgg gtgatgcact gagtgagggg cagtacacct cacagaacaa
     4261 cgtcaatggc atcacatcaa cactgaccgg cagtgccgga gaggcggcac ttggaaaagc
     4321 cctgtccggc gggatgaatg aaaccgcgga atgggtgaag cagcgatacg ggcagacgtt
     4381 tgatgccatt tatgttcccc cggggcagac gctggcggtg catatcacgc gtcaactggc
     4441 cattgattat gaagatcagg ggcgccgggt acgttacgac ttcactctgc cgggggaagt
     4501 ccacggtacc ggtggtctgg actgatgtaa ggagatcccg tgaaacgaca gttatctgta
     4561 atgaccgtca tggtggcggt atttattctg cccggctgct cgacatcgaa agaggagctt
     4621 ctgcctccgg gtgaacagag tatgctggag atctggcagg ggaaaggaag cggaggacat
     4681 tcgcggcaca tgacagcggc cagggacacc ctgcggcgtc cgctgacaat gccggagcag
     4741 caggcagatg cgcacggagc cataagctac agccggacac aggaaagtga gatcagtcag
     4801 cagtttccgc ggctgcccaa tcctgacatg gtgatgtatg tttttccgca tctggcttcc
     4861 ggtacgtcac cggttccggg gtacagcaca gtatttcctt tttacagccg gacgcagtat
     4921 gccatgccgg gtgaacgaac ggaggcgttg tgatgcgatt ttccctgttt acagacacaa
     4981 aaaaacgggt ttcttccccg gtgtcgcagc atgacggaga agcggtatct ccggatatga
     5041 aagaaagagt ggcccctcag gtacgaaagg cactgtcaag gccggggaag atgacccgac
     5101 aggatgagac gaaggtttac catgcctgtc cgtcggtgat tgattttctg ccatgggtgg
     5161 aatatctgga cgaagagcag tgcctgctgc tggatgatgg ggtgtcggtt ggtgccgtgt
     5221 atgaggtgag tccggccgct acggaagggc ggactgcaga gcgtctggag caaatccggg
     5281 atacggtgga ggatgcgctg caggacagtt ttgatgaata tgactcacat ccctgggtgg
     5341 tgcagttctt ctgtcaggat gaaaacgacg tggacacgta tgttgaccag ctcagggggt
     5401 acgtgaaacc tcatgcagaa ggctcctcat ttacggaggc ctggctgagg gagatggagc
     5461 gccatctgaa ggggattgcc cgaccggaag gtctgttcag ggatacgctg gtcacaggtc
     5521 agccatggcg tggtcagcag cggcgcacga ggatggtgat ttaccggtgg atcgggaaaa
     5581 acaaccacga ttcgatgccg ccggtggcaa tgctgaacca ggtctgttcg cgggtcgtgg
     5641 gggcgctggg tggcgcaggc gtccgctgta cacggatgaa cgggcagcag gtgcatggct
     5701 ggctgctgag gctgttcaat ccccggccgg aatgggtgga cagggatatt ctgtaccgta
     5761 tggcatcccg tgcagaaccg caggagacac cggagggaat gatgccggtg atgaccgatt
     5821 ttgcagaaag cctctggttc accccaccgg tatctgatcc ggaaaacggc gtgtggtggc
     5881 tggacgggct tccgcatgcc gctgttgtgg tggagaaact gcgcacacca ccggagccgg
     5941 gaaccatcac aggggaacag gcccggggcg aaaagacagt gaatgccctg atggacacct
     6001 ttccggaggg aacggtgctg tgtatgacaa ttgtggtcca gccacaggac acactggaag
     6061 agcgcttcac gaggttgtcg aaaaatgcgg tcggggagaa cagtgaatcc ctgcgtgccc
     6121 gggaggatgt ggcgaccgtg aagaaatatc tgggaaaccg gcacaagctc taccgtgccg
     6181 ggatcacgtt tctgctgcgg gcagaggata tggagagtct gaagcgcaga cgggttgaac
     6241 tgacgacggt gctgctgggc gcgggcttac agccagtgcg cccggagttt gatgtcggac
     6301 cgctgaacag ctggctgcgc gcgctgccca tgtgttttga tcctgatacg gataaaaaac
     6361 agtggtacac gcgtctgatg tgggtacagc atctggcagg gctcctgccg gtgacgggaa
     6421 gggaaaccgg tacggggcat cccggattca gtttctttaa ccgtggcggg gatgtgctga
     6481 cctttgatcc gctgaacaag ctggaccgga cccagaatgc acatctgctg cttttcgggc
     6541 ctaccggagc cggtaaatcg gccacgctgt gtggttcgtt gtcgcagata atggcagttc
     6601 atcgtccccg tctgtttatc gctgaggccg ggaactcgtt tggtctgctg gcggattatt
     6661 ttgagagtct cgggctttcg gttaataaaa tcagtgtcaa accgggaacg ggggtgtgtc
     6721 ttcctccatt tgcggatgct catcaactgg tggagcaggg agagacgctg cagagtgtgg
     6781 atgagcattc actgcccgat cttgatgaag atgaggggga tgaagaggaa gagaaacgcg
     6841 acattctggg ggagatggaa atctctgccc gcatgatgat caccggaggg gatccgaaag
     6901 aagaagcagc gctcaaacgt gcggacaggg cgatgatccg tgaggcactg ctgatggcca
     6961 cgcacaccac gtaccgggag gggcgtcaga tgctgccggt ggatctgcag agtgcgttgt
     7021 gggaaatttc gcgggacact cagcgtaatg atgttcgccg tgctaaagcc gcagagatgg
     7081 cagagtcgct ggggatgttc acccagccgg gcagttttga agcggagctg tttaaccggg
     7141 aaggaaaact gtggccggaa gcggatgtga ccctgattga tctggggcat cttgcccgtg
     7201 aaggttatga agcccagatg gcactgacca tggtgtcaat gaccaatatg attaacaaca
     7261 ttgccgagcg cgatcagtat ctgggacggg atattctgtt tgtggtggat gaggcgcata
     7321 tcgtgacggt aaacccgctt ttgtccccgt atatgaccaa agtggtgaaa atgtggcgca
     7381 agctgggggc ctggctgtgg ctggccacgc agaacctgaa ggattatccg gatgtctcgg
     7441 agaagatgct gaatatggca gagtggtggg tgtgcctgac catgccgccg gaggaagtga
     7501 acaatattgc ccgttttaaa tccctgacgg aggagcagaa ggcggtactg ctgtcagcca
     7561 gtaaactgtc cggctgttat acggaggggg tggtactggc gaagcgcaca gaagcgttgt
     7621 tccgggttgt gccaccgagt ctgtatctgg ctctggggat gacggagaaa gaagagaagg
     7681 ctgaacgccg cgccctgatg aaggtgcacc agtgcagcga actggaagca gcgtttcatg
     7741 tggcacgaaa gctggacggg ctacgtggtc tggctggcag ggagtgagac tatgctgtgt
     7801 ctgaagtatc cggaaccgga agtggtgtct gctgttcacc ccgccggaag tgtgtttgtt
     7861 ctgccgccac agggtgcgcc ggggataagt tgcaccacac gggataatct tgaacgtctt
     7921 cgcgggcatc tggcggcgca actggggcgg gtggagtgta tccgttgcca gccacagagg
     7981 gtgggtctga acagcagtgt ggcggtgatg ctggaaggtc aacagggtca gtatgtgcat
     8041 attctgctta ctgtgagtgg gcatgagagc tggccgtcgg aggaggagta tattcatccc
     8101 cgctggtata tatcagtgac tgatgcggcg gatttgttct atctgctgtt gtggctggga
     8161 gaggggtgat atgaggagcg atatcgattt tccgggacca ctgtatccat cgctgtcaga
     8221 tcaaattgcc agtgaacctg tgctgtttct ggcgggagct gtgggtgtgg ctgtgttttc
     8281 atgggtgctt gcgaatgtac tggcccggat gctgtcaggg ttgtactccc gtgtcaggag
     8341 gagggtaaag aactctgaaa aatagcggga gagaggtgct gccctgatgc cttcgttgta
     8401 ttctgcgacg ctgttggtgc ccgtattttg tgcgggtacc gtacagaatc accgcatgag
     8461 ccattgctga gacgctcact ttttttcgca tgcattaccg gagtgatggc tcagccggtg
     8521 attttgttgt tgagatgaga gccagtatgg gaaagcgata taaaataact gcgacaataa
     8581 tcaaaccagg caatatgccg gttcagtggc tgcgttacca ggatgaaaaa atgactcagg
     8641 ctgaatgtga aaaactgttt gcgcccgcaa aagagccggg acggtatatt ggcgtcaggg
     8701 tggaggactt ccggtgtgaa gtggtgaagg cctgagtatg aaaataaaaa acctccggcg
     8761 gagtcggagg gggcaaaaac agatagtccc ggagtgtatc tgtgtaaatg gtgttattgg
     8821 tataataatg gtggtaaaga ccacgggaga atttattcat tttattcgtc atgaaatcaa
     8881 cggtaatagt gtgtttctgt gacgggaata aaaaatacag cacagccgat gacccggtgc
     8941 tgaaaacagg ttaaatgtag tgggcacatt tccggtaata tcaggaacag catgttatgg
     9001 ttataaaagc atataccggg agtgtgccct gacactgtga tgttctggtt gtcttttgac
     9061 catttcccgg ggacgaaata taagctgttc tgcgttaaca cttaataatg agagattgta
     9121 tgctgaaaaa acaattctgg cgttatgtat ttccgcacca tttctggttg cgggctgtga
     9181 tgatgcgaca aaggagaatg taagtaagaa ggcggcagat ataaaggaag ctgcgcagac
     9241 tcaactcagt gatgtgaagg atgccgcgga ggaagtgaaa atgcaggctg ccggaaaggt
     9301 ggcagaggtt tctgaagcgg ctcagtccgg gctcagcgag gtgaaggata ccgctcctga
     9361 aaagggggct gaaatatccg taacggagga gtaaaagagc gccgtcggtg cagttttttt
     9421 cagcacccgg tgacacggaa ttttatatac ggggcatgtc tgtctcttta ataaatcaac
     9481 atatttaccg gatatccgat gccctgtacc tgcgttgtca ttatggctct caaacagaca
     9541 atgctttgtc tggagcacag gaactctgtc atacctgccg gcacaatgct tctgattgcc
     9601 gggcatctgc gccctgcttt tcaggctcat accggacgtt tccttatcgc tgacctcagt
     9661 gaggaggtgg tgaggcggta tctgcggttc tgcacgccat tatccggtat ggcagtcagc
     9721 ccgtcgtctt tgcttttaca gatccggcca cagatgtccg tgctggcgga atgtctgatc
     9781 agacaactta ttacagatga accatcgtcg gtgtacttca gggatgcggt ggctttttcc
     9841 agtctggcgc tgtttgagga acatccggat ttccacgccc tgctcacggg gtgtatccgg
     9901 agctgcactg tccgggtgaa gaatattatc agtgctgatt tgtcccgtcc atggatgctg
     9961 ggtgaagtgg ccagtctgtt atatataagt gagagtttac tgaagaaaaa gcttcaggaa
    10021 gagctgacga ccttcagtga gcttcttctg aaagagagaa tgcagtatgc cgctcagctt
    10081 ttgtctgcgg gagaaaaccg tatttcccgg gtggcagagt tgtgtggcta tgccagtgct
    10141 tcttatttta tttctgtgtt caggcgttat tatggtatta ccccgaatca gtggcttcct
    10201 gtgatgcaac cggattaatt tgattcagat gcagagtgtg cgttattcag aggaagaggc
    10261 ttaattttta tcatccggtt ttgaatttat tgctgattgt ccctttgcct ttgcgcacgc
    10321 tgtcagatac cagttgtctg acggagtcgc atcatgtacc gactgttact gtattccttc
    10381 ctgatactac cggtggctgc atctgccggt acaacaattt ataccgattc gcaccagcgc
    10441 cccatgaacc cgccagcagg tgttcgtgtt gtcctgcttg atgctcctga gcagacgcag
    10501 gatacatttt tcggggtact tcccgcagag cctgcagaag catctgcccg tgtcatggaa
    10561 cggatgcagt cgcctgaatg gcagtcgttt caggcagggc ttgctgaaca ctaccgtgca
    10621 ttagcacatg cctggtccct tggactgaaa aaatatccgg ctgtggtttt tgatgacagt
    10681 gaagtggtct acggaaccac agacgtggtg ctggcagaac aactgcgtac agggggaggg
    10741 cagccatgat ttcctgcaca tcacgtcatc gcgccggtgt tctgctgtta agtatggcgg
    10801 tattgccggt ggcagaggca tcgctgaaca ccgcgcagat agtggccagt gcagcctcac
    10861 agagctgtat cagctggcgg gtcagcggga tttgctactg gctgttctgc accaaattca
    10921 gttgcaaggt cagaacatca gtgaaagttt cccattatat cccggaagtg gttgtttcca
    10981 cctataccgc acctggcgga aatccctggc gtgaaatgtc ggcggtcagc agtacagccg
    11041 gtggtccgga gaatgccata agcaaaggta ttgccggtct gtcagcagga ggagggaacc
    11101 ccgcagacat gaaggtacag ggggaacgtc gttcgtcaat ccgttttaaa tatgccgatg
    11161 caacagggca cccggccacc acgcttatcg gagggatggt gccgggatat tcctgtgagt
    11221 caccggtgac accttttgtt ccttattttc tcagtacgct tgacagtatt gcctggcgta
    11281 caggactacc ggagtcactg tacccggagg ccctgatccc cggtaaacgc gaaataggca
    11341 gccaggaaga agagaatatg tggggcaatg tgtatccccg ctccgggttc gtgacccaga
    11401 cagatgatga taaagctgct gcggtggtgg cccagcgggt ggctgacatt atcacccgca
    11461 ccggtgaacc acatgtctat cagccggtga aagcagggaa gaaggacggt tactggccac
    11521 cggatccggt ggaggaaaat acgggcaccc ggaaccataa atggcagaga ctgacgcctt
    11581 cagtctccac gtcctgcgca gtcttccctg acggagagca tgaagcggct gaaacaggta
    11641 atgctgcctt tgccctgtgg cagccctaca gttgctgcaa gaaacgggga cagacattcc
    11701 tgggcagtac gaatttttaa gtaaggggaa gtgatgaaac agaaaatgac gatacctgtt
    11761 ctggctgtgg tgctttgtgc gggtacggta caggctgacg gaaacttttt gtccctgccg
    11821 aaagtgaatg acagtgcgtt tggctacggt aaatccgtgg acggcgcagt atcagacaaa
    11881 ctgttttata ccctgggagg gggctcggtg atatcgcagc cggcaacgcg cagcaatatg
    11941 cagaaacttg gggtggatct cgggtggagc agtgatctga tgtgtggcaa ttttgacctg
    12001 aaaacaacag tgggtaacca gctgaacggg atcactgatg gtttcaaaaa cctgatgggg
    12061 gatgtgattc agggggcgag cggagccgtg gcgagtctgc cggcaatggt gatacagcgg
    12121 gctaatccgg ggctgtatga catgctgacc aacggtgtac tgcaggcgaa tgtggcattc
    12181 gacaaagcgc agctcaattg tcagaacatg gcgaaacgca tgatggagtt cacggacagc
    12241 aataagtgga cgcaggcggc catgctcgag gagtacaaaa aaattgtgaa tacgggggac
    12301 ggtgatgcga tcaaagggga tgaagcaggt cgtcaggtta ccggggagga ggggcaacgc
    12361 tggatcggag ggcagcagcg tggtggagcg ggacagtctg ctatccggcc gacacatgat
    12421 ctgacagctg ccggtttcaa tatgatgaac agtctgcctt cgaccagtac atccagtgtc
    12481 agtcccggga actgtaacgg gagtgcctgc atgaaatacc gtaatgcaga ggaagctgcc
    12541 cgggcggtgg tgaaggttct gggggataaa tccatgcgga cctgtggtga tgcaaaggca
    12601 tgtaccagcg gggatgctga tcagcagccg gggaccagta ttgccggcac ggggtttgcg
    12661 ccgatgcttg aagaagcgac gcgggttaac acggaactgc tggcaggtat ggtcaatggt
    12721 cgtgacaagc cgacagccgg gaatctggca aaactgaaag ccgggagtct gtctgtgaca
    12781 gcgggagtga tccgtgccct gcagcgtgat ccggacaatg gtgccctgac gtcgcgtctg
    12841 gcgggagaac tggccatggc ggataccgtt gaaacggcgt tgctgatgcg caggatgctg
    12901 ctcacgggga tggccgagcc gtatgctgca gctcagcctg ctgcactgga agagggggaa
    12961 cgccgtattg aggcgctgga tcgggaaatt atggcgctga aaaacgagat ggagctgaag
    13021 cgggatctgg cgcgtaacag tgttctgacc atcattgaac gggataacga gcgtgtgggg
    13081 aataacccga tgatccagca ggctgacaat gctgacagcc gtatccattc tctcgatgcc
    13141 ccggataagg agtgagagga tgagcagaat ttatggccgg gtgaaaaaga ggatgaaggt
    13201 attcgccctg tatacgctgt tgctgacagg gattaccgtg ttttccctct ggctggccga
    13261 taccggtata cgtcacgcag aaggatttgc ggccctgcgg gagtggatgt atgagacacg
    13321 ttatggctgg ctgggatggc gtctgtgtct ttacagtgcg atcggatggc tggggtggag
    13381 gatctggcat gcgccggggt tcagggcaga ataccgggct tcttttctgc gtatggccac
    13441 agtcagtgtt ctgtttatcc tgctgtgtga atacgccatt tttgctgagc cggggcgttg
    13501 agcaatcatg accacaaata actatctgga atattttctg actctgctgg gctgggtggt
    13561 gaataacggt ctgtggaatg tgttgatcag cacaggactg tttgccctgc cactggcatt
    13621 caggatcatc gggatctggc tgaaagtcag ggaagaaggt gcggatgagg gaaacaaagg
    13681 gatgctttcc ctgccgcgta ttgagcatgt tctctatggt gctttttttg tgatgatatc
    13741 ctgctgtgtt cctctggtac aggtgacgct ggatacgctg tcattcgata aatccagggc
    13801 acagacctgt ggggtatgga cacctgaaga gcccggagag acggggtaca gtggggtgat
    13861 cagcagtata ggggatcaga ctgctgctgc cccggtatgg tgggtggtgg ttcataaatt
    13921 gtcgaaaggt gtcacccagg cggcagtggc ttcgattccg tgccggcctg atttgcgcca
    13981 gttgcgtttt gagatccagc gaacgtttat agaaaacagg gcacttgctg atgagctgca
    14041 ggactttacg aacgactgtt attcactggc actgtatatg tggaaacagc gtgatcaggg
    14101 gcagagtaaa gataaaaaag tgcttgatga tatctcctgg atagggagcg ccacattcat
    14161 gcagggggat tacgggacgc tgcagtcaaa gatgccgcgg gctgcgttcc cgtggaatac
    14221 agcacgtgac agcggacgac cggatacggg gcggggaggc tatccgacat gccgggagtg
    14281 gtggagtgac agtagtgtgg ggctgaaaaa acgggtcatg gaggaggtca gtgatcatta
    14341 ctggttacgt ctgtcagctg caatgaagat gattggcagc agtcaggtgg attataagga
    14401 ggaggtgatc aagcgtctgg tgagtccggc aaacctgacg ctctctcagg ggggagaggt
    14461 gtatcgtggc tatggtggca atgcagatat gacgaccatg aatgcggtgg cccggattgc
    14521 gggaacggcg ggcagtgcgg tagcagcact gggtgcgttt ccggcactgg atgcaatgcg
    14581 gcaggcactg ccgatggtac agtcgataat actgatggcg atttacatca tgataccgct
    14641 gattctggcg tttgcggcat atgaactgtc gacgttaatc acactgacgt ttgtggtgtt
    14701 tgcggtacac tttctgacat tctggtggga gcttgcacgg tggcttgaca gctggatgct
    14761 gacggcgctg tacagttcgg atacacatac ccggttcaat atgatgggat tccagaacac
    14821 atcggatgat ctgataatga atctggtgat ggggacgatg tttctcgtct tgccggcagt
    14881 gtggctggga gcgttgtcct gggctggggt aagtgtcgga acggttatgg gggatgcaat
    14941 taacaaagga acaagtaatg tatctaaggc agcagcaaaa agtggagatt tagctaataa
    15001 gtaaaattct tagggtttcc ttagctggga taattgggga aacccttgct tttcttagaa
    15061 atcagcattg tcatggtgat gtttccacgg gtcatataat gcaaagggtc tcattggtac
    15121 tgaataatca gtatcttttt cattacttag tgaaaacata ttttggggtt tatcttgagc
    15181 aggtgtgaaa tctggtaaaa cggcacagac tgcttttttc agcagggaag tgaaggtcca
    15241 tggattatcc acaggcttac aacgccacat tgctttttca gattcgttat tcatatctat
    15301 accctcagca gcaggataaa taacacagtg ttatcatacg tcttgtatga taaatgtgca
    15361 atcacatgtc ttccatttga tgagaggctg cgatgccaca ttacccttgg gctcggggat
    15421 tctgttgaaa tgatctagct gagaatgtat cttattgtta tgttataaca tgagtgaaga
    15481 gagatgaagc caaatatcca tcctgaatac cgcacagtag tgttccatga cacgagtgtg
    15541 gatgagtact ttaaaatagg ttctaccatc aaaacagatc gcgagattga actggatggt
    15601 gtgacgtatc cgtatgtgac gattgacgtt tcctccaaat cgcatccgtt ctataccggt
    15661 aaactcagaa cggtggcatc agaaggcaat gttgcccgct ttactcagcg ttttggtcgt
    15721 ttcgttggga ctcagaaggg agaatgatga gagtgagagt cttaacaggc tccatgcgtg
    15781 gtggatttgc tcctatattt ccagacatct ggtatcactt aacccattac tgcccgccgc
    15841 cgcagatatt cccgtggaga gcgataaccc agcgcactat gtggatgcca ttcgttataa
    15901 tgctcgaacg cttctgcaag gttctttgct gccgttaacc cgtctggttt gggcatgata
    15961 ctgatgtaga ttgaagaagg gattgtgagt ttttccttcg gcttttaacg taacttcctg
    16021 ttttcccgat attccacagc aagattaatt tttagcggag ctgaatgaat aaaatcttca
    16081 tctggtgttt ttgttgcgcg taaatgcagg tgtaactgtg gatgttatcc actgaagagc
    16141 tgctgatgag gatgaagaca tgctgactaa aaaaatgtta ctcgtttgtg cttcgtgtgt
    16201 tgcgctgagt gcctgttctg cacaggaaat tgcagagctg aacaggcaga taagtgacgg
    16261 tgcatttgct gtgacaaaag tactgaacgt aggtgtaaac caggcagatg atgatctcaa
    16321 atatctgtct caggcacccc gggcaaaaag agagaaacgt gatcatgtca tagaagtccc
    16381 tgttgatgtg gatacagctg cgggaagact gaaacggtac tataagtttt attcaaatga
    16441 agaagtcgat gcgattcgta ataacggaca tgcgaacggg aagtgggccg cagcagcaat
    16501 aagtcagaac gggcatatgt gggatgccat gcccggtagt tattataaaa tgggcagtga
    16561 ctgggatggt ggcgagtttg atgatcatct gacgcttgaa cttgaaaaaa atggcaaagg
    16621 aagtcggata tatataacgt atgcatctcc ggcaccgggg catctgaaag ataaatttat
    16681 tgagccatta gttaaacgtg tgaaggatgt ggctgaaggc cgggtcagat aaaattaacc
    16741 cgctggcatt gtcgttgtga tgggctcctc tggaggggcg attatgctca gaggggctga
    16801 aagtaaggga cgcagagggc gacgaccagc gacgtctgga agagggcgtg atatgtaagg
    16861 aagcacaccc tgacggcata tcataaaaaa accagatatg cgccagttaa aagtataaat
    16921 aaagagctga taaaattaac atatttt
//
LOCUS       DAZKHK010000110        16848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKHK010000110 DAZKHK010000000
VERSION     DAZKHK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.110"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..1430
                     /gene="dcuC"
                     /locus_tag="ACHOS5_003772"
     CDS             45..1430
                     /gene="dcuC"
                     /locus_tag="ACHOS5_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9778520.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1471..2151)
                     /gene="dpiA"
                     /locus_tag="ACHOS5_003773"
                     /gene_synonym="citB"
     CDS             complement(1471..2151)
                     /gene="dpiA"
                     /locus_tag="ACHOS5_003773"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP9778521.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2120..3778)
                     /gene="dpiB"
                     /locus_tag="ACHOS5_003774"
     CDS             complement(2120..3778)
                     /gene="dpiB"
                     /locus_tag="ACHOS5_003774"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP9778522.1"
                     /translation="MLQFKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4158..5216
                     /gene="citC"
                     /locus_tag="ACHOS5_003775"
     CDS             4158..5216
                     /gene="citC"
                     /locus_tag="ACHOS5_003775"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP9778523.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5231..5527
                     /gene="citD"
                     /locus_tag="ACHOS5_003776"
     CDS             5231..5527
                     /gene="citD"
                     /locus_tag="ACHOS5_003776"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP9778524.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5524..6432
                     /gene="citE"
                     /locus_tag="ACHOS5_003777"
     CDS             5524..6432
                     /gene="citE"
                     /locus_tag="ACHOS5_003777"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP9778525.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6443..7975
                     /gene="citF"
                     /locus_tag="ACHOS5_003778"
     CDS             6443..7975
                     /gene="citF"
                     /locus_tag="ACHOS5_003778"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP9778526.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLVEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            7979..8530
                     /gene="citX"
                     /locus_tag="ACHOS5_003779"
     CDS             7979..8530
                     /gene="citX"
                     /locus_tag="ACHOS5_003779"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP9778527.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLNIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8505..9383
                     /gene="citG"
                     /locus_tag="ACHOS5_003780"
     CDS             8505..9383
                     /gene="citG"
                     /locus_tag="ACHOS5_003780"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP9778528.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNISP
                     GGSADLLILTWFLAQI"
     gene            9434..10897
                     /gene="citT"
                     /locus_tag="ACHOS5_003781"
     CDS             9434..10897
                     /gene="citT"
                     /locus_tag="ACHOS5_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP9778529.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSRLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11011..11817
                     /gene="rna"
                     /locus_tag="ACHOS5_003782"
     CDS             11011..11817
                     /gene="rna"
                     /locus_tag="ACHOS5_003782"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP9778530.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYDDFDRYVLALSWQ
                     TGFCQSQYDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12047..12457
                     /gene="rnk"
                     /locus_tag="ACHOS5_003783"
     CDS             12047..12457
                     /gene="rnk"
                     /locus_tag="ACHOS5_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP9778531.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            12600..12689
                     /gene="yldA"
                     /locus_tag="ACHOS5_003784"
     CDS             12600..12689
                     /gene="yldA"
                     /locus_tag="ACHOS5_003784"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP9778532.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            12813..13241
                     /gene="uspG"
                     /locus_tag="ACHOS5_003785"
     CDS             12813..13241
                     /gene="uspG"
                     /locus_tag="ACHOS5_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP9778533.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(13362..14927)
                     /gene="ahpF"
                     /locus_tag="ACHOS5_003786"
     CDS             complement(13362..14927)
                     /gene="ahpF"
                     /locus_tag="ACHOS5_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9778534.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(15098..15661)
                     /gene="ahpC"
                     /locus_tag="ACHOS5_003787"
     CDS             complement(15098..15661)
                     /gene="ahpC"
                     /locus_tag="ACHOS5_003787"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9778535.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            16033..16779
                     /gene="dsbG"
                     /locus_tag="ACHOS5_003788"
     CDS             16033..16779
                     /gene="dsbG"
                     /locus_tag="ACHOS5_003788"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP9778536.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3994 a         4396 c         4201 g         4257 t
ORIGIN      
        1 gaatttttta ttatttactt tggggcctgg agacaggaaa aattatgctg acattcattg
       61 aactccttat tggggttgtg gttattgtgg gtgtagctcg ctacatcatt aaagggtatt
      121 ctgccactgg cgtgttattt gtcggtggcc tgttattgct gattatcagt gccattatgg
      181 ggcacaaagt gttaccgtcc agccaggctt caacaggcta cagcgccacg gatatcgttg
      241 aatacgttaa aatattgcta atgagccgcg gcggcgacct cggcatgatg attatgatgc
      301 tgtgtggctt tgccgcttac atgacccata tcggcgcgaa tgatatggtg gtcaagctgg
      361 cgtcaaaacc attgcagtat attaactccc cttaccttct gatgattgcc gcctattttg
      421 ttgcctgtct gatgtcactg gccgtttctt ccgcaaccgg tctgggtgtt ttgctgatgg
      481 caaccctgtt tccggtgatg gtaaacgttg gtatcagtcg tggcgcagct gctgctattt
      541 gtgcctcccc ggcggcgatt attctcgcac cgacttcagg ggatgtagtg ctggcggcac
      601 aggcttccga aatgtcgctg attgacttcg ctttcaaaac gacgctgcct atctcaattg
      661 ctgcaattat cggcatggcg atcgcccact tcttctggca acgttatctg gataaaaaag
      721 agcacatctc tcatgaaatg ttagatgtca gtgaaatcac caccactgcc cctgcgtttt
      781 atgccatttt gccgttcacg ccgatcatcg gagtgctgat ttttgacggc aaatggggtc
      841 cgcaattaca catcatcact attctggtga tttgtatgct aattgcctcc attctggagt
      901 tcatccgcag ctttaatacc cagaaagttt tctctggtct ggaagtggct tatcgcggta
      961 tggcagatgc atttgctaac gtggtgatgc tgctggttgc cgctggggta ttcgctcagg
     1021 ggcttagcac catcggcttt attcaaagtc tgatttctat cgctacctcg tttggttcgg
     1081 cgagtatcat cctgatgctg gtattggtga tcctgacaat gctggcggca gtcacgaccg
     1141 gttcaggcaa tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg gcgcactcct
     1201 ccggcattaa cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac ctgggtcgta
     1261 ccctatcacc cgtttctggc gtagtcgttg cggttgccgg gatggcgaag atctcgccgt
     1321 ttgaagtcgt aaaacgcacc tcggtgccgg tgcttgttgg tttggtgatt gttatcgttg
     1381 ctacagagct gatggtgcca ggaacggcag cagcggtcac aggcaagtaa atagtaatgc
     1441 cggaggcaag tttcctccgg ctttatcatg tcacccactg tggtatatgc gttgcggtct
     1501 gccaactttg ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc aatattcaag
     1561 ataacgcctg gcagtggtgc gactgatggt cagtgcctgc gccaccgttt ctgccgtatg
     1621 ttgcacacca ggctctttga acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc
     1681 ggtcggcagt tcgtctttgg gttcaccgcg agcataggca ttaaacatct catcaatttg
     1741 cttctggctg gcgctatcaa tactttccag catatgttta cgctggcgga aacgggttag
     1801 cgtttgcccc aaccgttcat aggcaattgg cttaatgaga taatcaaata caccacaacg
     1861 tacggcttca gacaccgttt ccatatcgct ggcagcagtg gtaaacacca cgtcgccggg
     1921 ataatgcgcc tgcaccagtt catgcagtaa attaatccct ctaccgtcag gaagatagtt
     1981 atcgagcaag attagccccg gcttaaaacg ctcgatcatc attcgggcct gcgccaggtt
     2041 tcccgccagt aatatctggc tgaatccggg aatgtgacga atatattccg catgcatctc
     2101 tgccagcggc gtttcgtcct caacgatcaa tagggttaat ggagctgtca ttaggtttca
     2161 ctttcggaat atagattgaa aataaggtac cgcagggatc attatcttcg agagtgataa
     2221 caccaccgca gcgcgttacg tagctggcaa tcaagtacaa cccaatgcca tgttcaccgg
     2281 gctcgtcagc acgcgtactg acaccctgct caaatatttt gtctcgtaga gactctggaa
     2341 cgccgcagcc ctgatcggcg acttcaatca ccacatcatc gccttcatcg ctgaggaata
     2401 attcaacgat cttgtttcct tcatcgctac gcaggcttgc ttcgaaggcg ttatcaagta
     2461 aattgccaac aatggctgca aactcggtgc tgtccagtcc tggcggcagt tgcgaaagct
     2521 ggctaccggg gacgatgacc atttttagcc ccagttcgcg ggcgcgctgc actttaccaa
     2581 aaagcagccc cgccacctgg cgatcggcaa acgcctcacg caggctgtca ataagctgtt
     2641 gctgggcctg agactccccc tgcaccatcg ccagcacgcg atcatactct ttcatctgca
     2701 acagaccatt gagcgtcgac atccaattga gatgctcgtg tcgcaatgta cgaaggctct
     2761 cgacgtattg tttaatttgc gtcagttgcg cattgagggt ggaaatttcg tctttactac
     2821 gaaagctaat aatggccccc agtaaatcat cgccagaacg aatagcttcc cggttggcaa
     2881 taacgctcag accgttaaag ttcgccacca catcctgacg tttttcatca atctgttcgg
     2941 taaagaaatc ggcgggcctg accacttcag caatgggttt acccaaccat tgccgtccgg
     3001 gggaactcag ccccagcatc tttcttgcgt tacgattgat ggcggtaatg tacccgtgcg
     3061 gatccaccgc aatcagccct tcataaaccg aactaaacag cgcctcttgc tgacggacta
     3121 cgcgagcgat ttgctttggc tccatgccca tcatctgccg acggatatgc gcggccagga
     3181 accacgacag caacatcaaa atccctaaca gcacgacaaa cacgcctgcc atcggtaata
     3241 aaaactcagc ccgccagcta tcgattttac tcaccagata gccaatcgac accacgccga
     3301 tgacttttcc atcgtcatca aagattggcg ttttggcacg catcgccatg ccaattgacc
     3361 ctttcccggt gatgaagtag ctctcccctt tctccagcgc gcccggtttg gtgaactgca
     3421 taggataacc aattttctcc ggattaggat ggtaaagacg aatcgagtgc cgatcgccaa
     3481 tcaccacata atcgaaatca gtatctcttt gtaatttgtc agcgatggtc gccagcctct
     3541 tgtagtcacg cgttttcact gcactgatga tactgtcatt agaagcaata atttttgcct
     3601 gattcattgc catgtcgcgc acatgaagcg ttaaatagtc ctcaaaactg gctgtaaaat
     3661 attgcgccag ggctgcaatg acaaatattg agaacaccag aatcagcaaa aagatacgca
     3721 gcggaaatgc cagtctttgg aaaaatgcaa actgtttatt ctctttaaac tgcaacattg
     3781 ttttccttgc gggttgtccc gagcctcttt gacggatgta attaaaatgt tgaatcaaaa
     3841 tacatagata ttaaatatga tcgtggcaaa ttttattcat taaatcaatt aaatcactta
     3901 aacctgcggg ttatttcgta attacattag ttaaaaaact taaaaccatt aaataaataa
     3961 aaaaccacta actctatgtg aaataaatca aaatttcacg ccgaaatact ccttaggatg
     4021 tatagcgaaa ggagaaaaag atatacctcg atcgcccccc tttctcccaa gtgaaaataa
     4081 aaggttatca aattgcaaca ttgaacaaca ttcgttgcaa atcgataaca acatgcacct
     4141 tcaggatact atttattatg ttcggcaatg atattttcac ccgtgtaaaa cgttcagaaa
     4201 ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa tgatttgagc gttgacacca
     4261 cagtcgaagt atttattacc gtaacccgcg atgaaaagct tatcgcgtgc ggtggaattg
     4321 ccggaaatat tattaaatgc gttgctatca gtgaatccgt tcgcggtgaa ggactggcgc
     4381 tgacattagc caccgagcta attaacctcg cctatgagcg gcacagcacg catctgttta
     4441 tttataccaa aaccgaatac gaggcgctgt tccgccagtg cggtttttcc acgctgacca
     4501 gtgtacccgg cgtgatggtg ctgatggaaa acagcgccac gcgactgaaa cgctatgccg
     4561 aatcgctgaa aaaatttcgt catccaggga acaagattgg ctgcattgtg atgaacgcca
     4621 atccctttac gaatggtcac cgttatctga ttcaacaggc tgcggcacag tgcgactggt
     4681 tgcatctgtt tttagtcaaa gaagattctt cacgcttccc ctatgaagac cggctggatc
     4741 tggtgttaaa aggcaccgcc gatattccac gcctgaccgt gcatcgcggc tccgaataca
     4801 tcatctcccg cgctacgttc ccttgctact tcattaaaga acagagcgtc attaaccatt
     4861 gttacaccga aattgatctg aagattttcc gtcagtacct cgctcccgcg ctgggtgtaa
     4921 ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac cgcccagtac aaccaggata
     4981 tgcgctactg gctggaaacg ccgactatct ccgcaccgcc catcgaactg gttgaaattg
     5041 agcggctgcg ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa ctgctggcga
     5101 aaaacgatct cacggctatc gcgccgctgg tccctgcagt cacgctgcat tatttgcaga
     5161 acctgcttga gcactcccgc caggacgcgg cagctcgtca aaagaccccc gcatgagaaa
     5221 caggtgaaaa atgaaaataa accagcccgc cgttgcaggc acccttgagt ctggggatgt
     5281 gatgatacgc atcgccccac tcgatacgca ggatatcgac ctgcaaatca acagcagcgt
     5341 tgagaaacag tttggcgatg caattcgcac caccattctg gacgttctcg cccgctacaa
     5401 cgtgcgcggc gtacagctga atgtcgatga caaaggcgca ctggactgca ttttacgtgc
     5461 acgactggaa gccctgctgg cacgcgccag cggtatcccg gctctgccat gggaggattg
     5521 ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag tatgttgttt
     5581 gtgcctggtg ctaatgccgc gatggtcagc aactccttta tctacccggc tgatgccctg
     5641 atgtttgacc tcgaagactc cgttgcattg cgtgaaaaag acaccgcccg ccgcatggtt
     5701 taccacgcgc tgcaacatcc gctttatcgc gatattgaaa ccattgtgcg tgtaaacgcg
     5761 ctggattccg aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg tgcggacgtt
     5821 gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg atatcgaaaa agagatcctg
     5881 cgtatcgaaa aagcctgtgg tcgtgaaccc ggcagtaccg gcctgctggc ggcgattgaa
     5941 tctccgctgg gcattacccg cgcggtagaa atcgctcacg cttccgagcg tttgatcggt
     6001 atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc cccggaagga
     6061 actgaactgc tgttcgctcg ctgctccatt ttgcaggccg cgcgttctgc gggtattcag
     6121 gcgttcgata ccgtctattc cgacgctaac aacgaagccg gattcctgca agaagccgcc
     6181 cacatcaaac agctgggctt tgacggcaaa tcgctgatca acccgcgtca gattgatctg
     6241 ctgcacaacc tctacgcacc gacccagaaa gaagtggatc acgcccgccg cgtcgtagaa
     6301 gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg caagatggtg
     6361 gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact ttccggcatc
     6421 cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa caatctcaac gacaagaacg
     6481 ggtagcggcc tggaatcgtc gcgctgaatg cgatcttgcc gctttccaga actcgccaaa
     6541 gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc tggaagaagc
     6601 gattcgtcgc tctggtttac aggacggcat gacggtttcc ttccatcacg ctttccgtgg
     6661 cggtgacctg accgtcaata tggtgatgga cgtcatcgcg aagatgggct ttaaaaacct
     6721 gactctggcc tccagctccc tgagtgattg ccatgcgccg ctggtagaac acattcgcca
     6781 gggcgtggtt acccgcattt atacctctgg cctgcgcggt ccactggcgg aagaaatctc
     6841 ccgtggtctg ctggtggaac cggtgcaaat ccactctcac ggcggacgtg tgcatctggt
     6901 acagagcggc gaactgaata tcgatgtggc tttcctcggc gtcccgtcct gtgatgaatt
     6961 cggtaatgcc aacggctact ccggtaaagc ctgctgcggc tccctcggct atgcaatggt
     7021 tgatgccgac aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc cttatccgca
     7081 taatccggca agcattgagc aagatcaggt tgatctgatc gtcaaagttg atcgcgttgg
     7141 cgatgctgca aaaatcggcg ctggtgctac ccgtatgacc actaacccgc gcgaactgct
     7201 tattgcccgt agcgctgcgg atgtgattgt taactctggc tacttcaaag aaggtttctc
     7261 catgcagacc ggcaccggcg gcgcttcgct ggcggtaacc cgtttcctgg aagacaaaat
     7321 gcgtagccgc gatattcgcg ccgacttcgc cctcggcggt attaccgcaa cgatggttga
     7381 tctgcacgaa aaaggtttga tccgcaaact gctggatgtg cagagctttg acagccatgc
     7441 tgcgcaatcg ctggcccgta accccaatca catcgaaatc agcgccaacc agtacgctaa
     7501 ctgggggtcg aaaggcgcag cggttgatcg tctcgacgtg gtggtactga gcgcgctgga
     7561 aattgacacc cagttcaacg ttaacgtgct gaccggctct gacggcgtac tgcgtggtgc
     7621 ttccggtggt cactgcgata ccgcgattgc ttctgcgctt tccatcatcg tcgcgccgct
     7681 ggtacgcggt cgtattccga cgctggtgga taacgtgctg acctgcatca ccccgggctc
     7741 cagtgtcgat attctggtca cagaccacgg catcgctgtt aacccggcac gtccggagct
     7801 ggcagaacgt ctgcaggaag cggggatcaa agtggtttct attgagtggc tgcgtgaacg
     7861 tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc accgaccgcg tcgttgccgt
     7921 tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat caggtgaagg aataagccat
     7981 gcacctgctt cctgaactcg ccagccacca tgcggtgtca attcccgagc tgctcgtcag
     8041 ccgggatgaa aggcaagcac ggcaacacgc ctggctcaag cgccatcctg ttccactggt
     8101 ctcctttacc gtggttgcgc ctgggccgat taaagacagc gaggtcacac gccgaatttt
     8161 taatcatggc gtgacagcct tgcgtgcctt agccgcaaaa cagggctggc aaattcagga
     8221 gcaggctgca ctggtttccg ccagcgggcc ggagggcatg ttgaacattg ccgccccggc
     8281 tcgcgacctc aagctcgcca ccattgagct tgaacatagt catcctctcg ggcggttatg
     8341 ggatatcgat gtcctgacgc ccgaaggcga cattctctcc cgccgcgatt attcactgcc
     8401 gcctcgccgc tgcctgttgt gcgagcaaag cgcagctgtt tgcgcgcgtg gaaaaaccca
     8461 tcaactgacc gatttactca accgcatgga ggcgctgcta aacgatgtcg atgcctgcaa
     8521 cgtcaactaa aaccacaaag cttgcgacgt cattaatcga tgagtacgcc ctgctgggct
     8581 ggcgcgccat gctgactgaa gtcaacctgt caccgaaacc aggcctcgtg gatcgcatta
     8641 actgcggtgc gcacaaagat atggcgctgg aagatttcca ccgcagcgcg ctggcgattc
     8701 agggctggtt accccgtttc attgaatttg gtgcctgtag tgcagaaatg gcaccggaag
     8761 cggtactcaa cggattacgc ccaattggta tggcttgcga aggtgatatg ttccgcgcca
     8821 ctgcgggcgt aaacacgcat aaaggaagca ttttttcttt agggctactg tgtgcggcaa
     8881 ttggccgttt gcttcagctc aaccagccgg taacgccaac aaccgtttgt tctacggcgg
     8941 caagtttctg ccgtggcctg accgatcgcg aactgcgtac caataattca cgactgacgg
     9001 caggtcaacg gttgtaccag cagcttggcc ttaccggcgc acgcggtgaa gccgaagcgg
     9061 gttatccact ggtgatcaac cacgccttgc cgcattacct cactctgctg gatcaggggt
     9121 tagatcctga gctggcattg ctcgatacct tgctcctact gatggctacc aacggcgata
     9181 ccaacgttgc atcgcgcggt ggcgaggggg gcctgcgctg gctacagcgc gaggcgcaaa
     9241 cattattaaa caatgggggc atccgaaccc ccgccgatct cgattatctc cggcagttcg
     9301 acagggagtg tatcgaacga aatatcagtc caggcggcag tgccgacctg ctgattctta
     9361 cctggttttt agcacagatt taattattta agcacttgat aaatttggaa atattaattt
     9421 tcggagaacc cgtatgtctt tagcaaaaga taatatatgg aaattattgg cgcccctggt
     9481 ggtgatgggt gtcatgtttc ttatccctgt acctgacggt atgccgccgc aggcgtggca
     9541 ttactttgca gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac
     9601 ggcgatcagt tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga
     9661 tgccaaagaa ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct
     9721 ggctggtttt tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg
     9781 gtatgaagtt tccaggttag gccgtcgtat tgcccttttc ctggtgaaat tcatgggcaa
     9841 acgcacgctg acgttgggtt atgcgattgt cattatcgac attctgctgg caccgtttac
     9901 accgtccaac accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc
     9961 gctgtttaaa tcattcccga acgatccgtc cgcgcgtcgt attggcggct atttgatgtg
    10021 gatgatggtc attagtacca gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa
    10081 cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc cagattagct ggttgcagtg
    10141 gttcctctgc ttcctgccgg ttggggttat cttgcttatc attgcgccgt ggctttccta
    10201 cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg gcaacctggg cgggtgatga
    10261 actaaaaacc atgggtgcgc tgacacgcag agagtggacg ctgattggcc ttgtattgct
    10321 cagcttaggt ttgtgggtat ttggcagtga agtcattaat gctactgcgg taggtctgct
    10381 ggcagtttcg ctgatgctgg cgctgcacgt tgtgccgtgg aaagacatta ctcgctataa
    10441 cagcgcatgg aacacgctgg tcaacctggc aacgctggtt gttatggcga atggtctgac
    10501 ccgttctggc tttatcgact ggttcgccaa cacgatgagt acgcacctgg aaggcttctc
    10561 accaaacgca acggtgattg tactggttct ggtgttctac tttgcccact acctgtttgc
    10621 cagcctgtct gcgcacaccg caaccatgct gccggttatt ctggccgtcg gtaaagggat
    10681 tccgggcgta ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg
    10741 ctgtctgacg ccttatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa
    10801 atcaaaagat tactggcgtc ttggcgcaat cttcggggtg atttacatct ctatgctgct
    10861 gttggttggc tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa
    10921 atcatcacgc ccgccaggta agcccacctg gcgggctttt ttatgattta atagataata
    10981 ttcagtgtat gtatgagttc cacacccatt atgaaagcat tctggcgtaa cgccgcgttg
    11041 ctcgcggttt ctctgcttcc cttctcttct gccaacgccg tggcgttgca ggcaaaacag
    11101 tatgacgatt ttgatcgcta tgtcctggcc ctctcatggc aaaccggatt ttgtcagagt
    11161 caatacgacc gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac
    11221 aaagctgatt ttttgaccgt acatggtctg tggccaggat tgcctaaatc ggttgctgcc
    11281 cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat
    11341 ctgccagaag cacgtgccag ccggatgtgt tcatcgccgg aaaccgggtt atcactggaa
    11401 acggcagcta aactaagtga agtcatgcca ggagctggcg gacgttcctg cctggaacgc
    11461 tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc cagacgcata cttcggcacg
    11521 atggtacgcc tgaatcaaga aattaaagag agcgaagccg gaaaattcct tgcggataat
    11581 tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct ttgccaaaag ctggggaaaa
    11641 gagaacgtga aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt
    11701 cagatctcga tcaaagctga cgccatcaac gctccgcttt ctgccaactc attcttgcca
    11761 caaccacacc caggcaactg tggcaaaacc tttgtgattg ataaagcagg ttattaatcc
    11821 tctgatttac aaaacacgct aattcgtctt ccataaacca tgacgattaa gatttataca
    11881 taatgcgcta cattgaggaa ttccctgaat gtgacgcaaa tcacttcaaa gagtgagcaa
    11941 cagtcagtat catcatgtaa gttaaaccct cgccgcctga cggtgagggt tttcttttgg
    12001 gtatgtttat ctgcgacact cgcagtaccg acgacatgga gtaaaaatgt ccagaccaac
    12061 tatcatcatt aacgacctgg atgccgaacg catcgatatt ctgctggagc aacccgccta
    12121 tgctggtttg ccaatcgccg acgcgttaaa cgcagagttg gatcgcgccc aaatgtgttc
    12181 gccagaagag atgccacacg acgtggtgac aatgaacagc cgggttaaat tccgcaatct
    12241 tagcgatggc gaagtgcgtg tgcgcacgct ggtgtatccg gcaaaaatga ccgacagcaa
    12301 tactcagctt tccgttatgg ctccggtagg tgccgcactg ctggggctgc gcgttggcga
    12361 ttccattcac tgggaacttc cgggcggcgt tgcaacccac cttgaagtgc tggaactcga
    12421 ataccagcca gaagctgctg gcgactacct gctttaatcc catctgcaca agcgataact
    12481 cgctttgccc agaggatgca ctcgcatcct ctttccgctc aaatatcaac cagatttatc
    12541 attttgcgct taagcgcagc attctaatct gaactggtta ttcatctgcg gaacctcata
    12601 tgagcgaagc tctcggtatc actgcatttt atatattcat agcagcaatt attgttgctg
    12661 cagtgctata ccttgaacaa cgctggtagc gcctggtttt atttattttc cagctcaata
    12721 gcaaattcag atcaaatcag cctcgcaaat tatagattca taaccaataa tgtgctgtta
    12781 tgtatacatg caaaaacaag gagagaatgc ttatgtacaa aacaatcatc atgccagtgg
    12841 atgttttcga aatggaattg agcgacaaag cgattcgcca cgctgaattc ctcgcccagg
    12901 atgacggagt tattcatcta cttcacgtac taccggggtc agccagcctg agcctgcacc
    12961 gttttgccgc tgatgtacgt cgttttgaag agcatctgca acatgaagca gaagaacgtt
    13021 tgcaaacgat ggtcagccac ttcaccatcg atccttcccg cattaagcaa catgtccgtt
    13081 ttggtagtgt gcgggatgaa gttaacgagt tagcgaaaga acttgatgca gatgtagtag
    13141 tgattggttc gcgtaaccca tcaatttcaa ctcatctgtt gggttctaac gcctcgagcg
    13201 taatccgcca cgccaatttg ccggtgctgg ttgtgcgtta attgccagaa taagtctccc
    13261 gccctgcccg gagccatccc tgcagggctt tttaatgcaa taaaaaagcc gccaggtttg
    13321 accccggcgg ctaagcaatt acaggtgaat cttacttctt cttatgcagt tttggtgcga
    13381 atcaggtagt caaaagcact cagagaggct ttggcacctt cgccagtggc gatgatgatc
    13441 tgcttgtacg gaaccgtcgt acagtcaccc gctgcgaaca cgcctttgac gttggtttcg
    13501 catttcgcat caatgataat ctcgcccatg cgattacgtt cgactgcgcc ttccagccag
    13561 ttggtgttcg gcagcagacc aatctggacg aaaataccgg ccagttcgat gttgtgaata
    13621 tcgccgctga cacgatcgcg atattccaga cctacgactt tgctaccgtc gcctttcact
    13681 tccgtggttt gcgcattcag aataatgtcg acgtttttca ggctgcgtag tttgtcctgc
    13741 agaacctggt cggctttcat ttctggcgca aattccagca gcgttacgtg ctcaacgata
    13801 cccgccaggt caattgccgc ttccacgccg gagttaccgc cgccgataac cgctacgcgt
    13861 ttgcctttaa acagcgggcc gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac
    13921 tgatcttcgc caggaacgtt catgttgcgc cattttgcac cagtcgccac gataatgctg
    13981 cgtgctttca gtaccgcgcc agaagctgtt tcaatctgat gcaggccgcc ttcaaccgct
    14041 gccgggatca gtttgctcgc gctctggctg tcgatcacat caacgtcgta ttcatcaaca
    14101 tgaactttca gcgcacctgc cagtttctgg ccttcggtct tcggtacaga aatgtagttt
    14161 tcgatatcaa cggtatcgag gatctgacca ccaaaacgtt cgcccatcag accggtacgg
    14221 atgcctttac gtgcggagta aattgctgcc gctgcacccg ccgggccgga accaacgatt
    14281 aatacgtcat aagcatcacg cttgttcagc tcttctgccg cacgtttttc cgcaccagta
    14341 tcaattttgg caacgatttc agtcaacgtc atgcggccct gaccaaactc tttcccgttt
    14401 acgaacactg ccggaacgcc catcacgttg cgatcggtga tttcgttctg gaaggtgccg
    14461 ccgtcaattg cagtgtgctt gatgcgcggg ttcagtacgc tcatcaggtt cagcgcctgc
    14521 accacgtcag ggcagttgtg gcaagagagc gagtaatagg tttcgaattc aaaatcaccg
    14581 tcaatatggc gaatctgctc cagcagagac tgcgcttctt tcgacggatg accaccggtc
    14641 cacaacaacg ccagtaccag cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt
    14701 ggtccctggt tggaacctgg gttggtgatc aggaaagacg gcttacgcac tggcaagctg
    14761 ttatcttctt taaaggtgac tttgtctgac agttctgcga tttcagccaa cagttccttg
    14821 atttctgccg atttagcgct gtcatccagc gtggcaatta actcaacagg cttggtcaat
    14881 ttctcaaggt aagccttgag ttgagttttc atatttgtgt cgagcatgtt tatctcctgg
    14941 gctttaaaca tcatcatgca agcggcctct gcggctagct ggatgcagct tgcatcatgg
    15001 tgcggggaag aaaatcgggt gccgcagcac ccgaagaatt aggtgaattt ccgaagtatc
    15061 tcgcgccgga gttagtagcg cgaaagacaa aggaaattta gattttacca accaggtcca
    15121 gagacggagc cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt
    15181 gagaagctac gtactgtgct gctttgattt tacgcagcag gtcagacgcg tcacggccaa
    15241 tgccttcagc ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg
    15301 cacggtcagc cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag
    15361 tcgggtcgcc gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg
    15421 ctttgtgggt gaagtgggta tcggtagata ctgcgtatac gtctacgccc agtttctgca
    15481 gttcttcgta gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag
    15541 ccgggtagaa gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga
    15601 attcgccgtt tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca
    15661 tctatacttc ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct
    15721 aatgcgtttc cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg
    15781 tgaactttgc accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg
    15841 taatctgaac taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta
    15901 caaatgcaac aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct
    15961 aagctcttac ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa
    16021 aaggacaaat taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc
    16081 gcagaggaac ttcctgcccc agtaaaagcg attgaaaaac agggtattac aatcatcaaa
    16141 acgttcgatg cccctggagg aatgaaaggt tatctcggta agtatcagga tatgggcgtc
    16201 accatctacc tgactccaga tggtaagcac gctatctctg gttacatgta caacgaaaaa
    16261 ggtgaaaacc tgagtaacac gcttatcgaa aaagaaattt acgcacccgc cggacgtgaa
    16321 atgtggcaac ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga cgcgccggtc
    16381 attgtctacg tcttcgccga tccgttctgc ccatattgta aacagttctg gcagcaggct
    16441 cgtccgtggg tagattctgg caaagtgcaa ttgagaacat tgttggttgg ggttatcaag
    16501 ccagaaagcc cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg
    16561 caacaatatg aagcctctgg tggcaagctt aagctaagcg tgcctgcaaa cgtaagcaca
    16621 gagcaaatga aagtgttaag cgacaacgag aaactcatgg acgatctggg ggcaaatgtc
    16681 acaccagcta tctattacat gagtaaggaa aataccctgc aacaagccgt cggattaccc
    16741 gatcagaaaa cacttaatat cattatgggg aataaataga atataattca tcgatggttg
    16801 aaatctctct ctgcttttca accatcctaa aaacgcatga attgctcg
//
LOCUS       DAZKHK010000111        16715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKHK010000111 DAZKHK010000000
VERSION     DAZKHK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.111"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            114..2753
                     /locus_tag="ACHOS5_003789"
     CDS             114..2753
                     /locus_tag="ACHOS5_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP9778537.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQAEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNFQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            2750..2935
                     /locus_tag="ACHOS5_003790"
     CDS             2750..2935
                     /locus_tag="ACHOS5_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP9778538.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2943..3269
                     /locus_tag="ACHOS5_003791"
     CDS             2943..3269
                     /locus_tag="ACHOS5_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP9778539.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            3645..7853
                     /locus_tag="ACHOS5_003792"
     CDS             3645..7853
                     /locus_tag="ACHOS5_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HFP9778540.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPTPPKPISFNNDVILDKTEKTLTIRD
                     SVFTYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGEL
                     VITGDNTTVNNTGKTIVDGKGATGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATV
                     DNKGGMTVTDPDSIGIQIDGDKAVVNNDGDSAISNGGTGTQVNGDEATVNNNGSTTVD
                     GKDSTGTEINGDKAIVNNDGDNTILDGGTGTRITGDDATANNSGNTTVDGQGSTGTEI
                     AGNNAVVNQDGELDVSGGGHGIDITGDSATVDNKGGMTVTDPDSIGIQIDGDKAVVNN
                     DGDSAVSNGGTGTQVNGDEATVNNNGSTTVDGKDSTGTEINGDKAIVNNDGDSTILDG
                     GTGTRITGDDATANNSGNTTVDGQGSTGTEIAGNNAVVNQDGELDVSGGGHGIDITGD
                     SATVDNKGGMTVTDPDSIGIQIDGDKAVVNNDGDNAISNGGTGTQVNGDEATVNNNGS
                     TTVDGQGSTGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATVDNKGGMTVTDPDSI
                     GIQIDGDKAVVNNDGDSAISNGGTGTQVNGDEATVNNNGKTTVDGQGSTGTEIAGNNA
                     VVNQDGELDVSGGGHGVDITGDSATVDNKGGMTVTDPDSIGIQIDGDKAVVNNDGDSA
                     ISNGGTGTQVNGDEATVNNNGKTTVDGQGSIGTEIAGNNAVVNQDGTLDVSGGGHGID
                     ITGDSATVDNKGGMTVTDPDSIGIQIDGDKAVVNNDGDSAISNGGTGTQVNGDEATVN
                     NNGKTTVDGKDSTGTEINGDKAIVNNDGDSTILDGGTGTRITGDDATANNSGNTTVDG
                     QGSTGTEIAGNNAVVNQDGELDVSGGGHGIDITGDSATVINKGNITVTDKDSVGVLIN
                     GDRATFANTGHIDVNNSATGFSIATSEGVISLTGSMDVGDISTGMVLSGDGNSVTLAV
                     EDLNVTGQKAMGVDISGDSNDIDIAGNILVDKDQTADNAADYFFESSVGVNVTGNNNT
                     VSLNGELTVVSDSELTSRSHGKRDGSQENISGLIVSGDNNTISLNGGIQFIGEQKILA
                     DGSDIASLRKGFSDTSIISVDGNSSVYLNGTSTIGGDLPLGYTSIIQLKNKAILEIGR
                     DALLGVKDIKSFNNYYEPVPKIIKATTGSKVINNGNIDIQNIGFISVSGEDTSGTNNG
                     NITLSQYDYGNMMSGTNALLSTDGGSVVNNGTIIGKVMEQSSVINRFETTDVTYDELF
                     NNSVTGITGMLSKTGAMGINNVNY"
     gene            7879..10854
                     /locus_tag="ACHOS5_003793"
     CDS             7879..10854
                     /locus_tag="ACHOS5_003793"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9778541.1"
                     /translation="MLAVTQAIIENAGTIKLDSLWVDANDTTALPDNIAISNARYYGA
                     GMAVGSDSYGSPDANVTAINQLGGVISVYNAGVGMAAYGGSNMVINQGTINLEKNENY
                     SSSLSANTLVGMAVYQHGTAINDKTGVININVDTGQAFYNDGTGTILNYGEINLLGSP
                     MDSADPHMGATPDNLDLLSALTGSGETDMRTASSGGFVTTKALANYGNETLNSNVTAK
                     AWLYNQDKANLTINGELSVGQGLENSGLLDSDTISAAANVYNRASGSIITDLLMLTSG
                     NSFFNEGNFSGSIVGNSYRQNVVNTGSMTVTADGTSLIGGSFVLYNEAGAILSNSNSA
                     VSGGENAIVNITRTSDSLAQVNRGTITATNGYSAIKTASTGSNSNGKWIWNTDTGVIS
                     GVNPNAPLIDLGRGYNFANAGTINVQGDGAVAISGGTTSYTVQLVNSGTINVGTAQGK
                     ADGTNGTGLIGIKGNGRETTINNAQSGVINVYADNSWAFGGQTKTIINNGEINLLCDM
                     GCDIYAPGTTGTLNDHNSTTDIIVPAATSTPTQGSVPTVPADSSAQQKLTNYTIGTNS
                     DGTSGMLKANNLVISDNVKVNTGFSAGTADTTVVINDVFKGENISGAENISSSTVMWN
                     AQGSTDASGNVDVTMTKNAYTDVVTDSSVNNVAQVLDAGYTNNELYSSLNVGTTAELN
                     SALKQVSGSQATTVFNEARVLSNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNNTQ
                     YDMMALRKSMTLTEHQNLSLEYGIARLDGNGSDTVGDNGVTGGYSQFFGLKHQMAFDN
                     GMSWNNALRYDVHNLDSSRSIAYGDVNKTADANVKQQYLEFRSEGAKTFELREGLNVT
                     PYAGVKLRHTLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKLDYAGKEGWSANATLE
                     GGPNLSYVKSQRTASVSGAGSQRFNIDDGQNGGGFNSLATMGVKYSSQESALQVDAFH
                     WKEDGISDKGVMLNFKKTF"
     gene            11074..11940
                     /gene="pdxI"
                     /locus_tag="ACHOS5_003794"
     CDS             11074..11940
                     /gene="pdxI"
                     /locus_tag="ACHOS5_003794"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP9778542.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPTEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSIAQKAG"
     gene            complement(11981..12586)
                     /gene="azoR"
                     /locus_tag="ACHOS5_003795"
     CDS             complement(11981..12586)
                     /gene="azoR"
                     /locus_tag="ACHOS5_003795"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP9778543.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTSKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            12787..16689
                     /gene="hrpA"
                     /locus_tag="ACHOS5_003796"
     CDS             12787..16689
                     /gene="hrpA"
                     /locus_tag="ACHOS5_003796"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP9778544.1"
                     /translation="MTEQQKLTFADLQQRLDSLMLRDRQRFLRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDLGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         4424 a         3885 c         4581 g         3825 t
ORIGIN      
        1 attcatcaac aactatgctt agtgtaggcg caaccttcaa ctgaacggtt aaacatgcca
       61 caatacccgt attgaatgct taatttttcg ctaaatcagg atattaacta cccatgctgg
      121 gtaaatataa agccgttctc gcgctgttat tactgattat tcttgtgccg ttgacgctgc
      181 tgatgacgct cgggctgtgg gttcccacgc tggcgggcat ctggctaccg ctcgggacac
      241 gtattgcatt agatgaaagc ccacgcatta cgcgtaaagg tttaatcatc cccgatctcc
      301 gttatctggt gggagattgt cagcttgcgc atatcaccaa cgccagcctt tcacatccca
      361 gccgctggtt attgaacgtc ggcacggtag aacttgattc tgcttgtctg gcgaaattgc
      421 cgcaggcgga acaatcgcca gccgctccaa aaaccctcgc gcagtggcag tccatgctgc
      481 ctaacacctg gatcaatatc gataaactga ttttttctcc ctggcaggaa tggcagggaa
      541 aactctctct cgcattaacc tccgatatcc agcaactgcg ttatcagggc gaaaaagtta
      601 aatttcaagg ccagttgaaa gggcaacaac ttacagtcag cgaactggat gtcgccgcgt
      661 ttgaaaatca gccgccggta aaactggtgg gggaatttac tatgccgctc gtgccggatg
      721 gacttccggt aagtggtcat gctactgcga cgttaaactt gccgcaggaa ccgtcactgg
      781 tggatgccga gctggactgg caggaaaata gcgggcaatt gattgtgctg gcacgggata
      841 acggcgatcc gttgctcgat ttgccgtggc aaattactcg tcaacaattg accgtaagcg
      901 atggtcgctg gagctggccg tatgcaggtt ttcctttgag tggccgactg ggtgttaaag
      961 tcgacaactg gcaggcaggg ctcgagaacg ctctggtcag cgggcgactg agtgtgctga
     1021 cccaggggca agcgggtaaa ggcaacgcgg tgttaaattt tggcccagga aaattaagca
     1081 tggataacag ccagctgcct ctgcagctga ccggtgaagc gaaacaggcg gacctcattt
     1141 tatatgcccg tttacctgcg cagctaagtg gaagtctgtc tgacccaacg ctgacctttg
     1201 agcctggcgc gttacttcgt tcaaagggaa gagtcattga ttcgctggac atcgatgaaa
     1261 tccgctggcc tttagcgggt gtaaaagtca cccaacgtgg tgttgacgga cgtttgcagg
     1321 ccattttgca ggcgcatgaa aatgaactgg gcgatttcgt gctgcatatg gatgggctgg
     1381 cgaatgattt tctccctgac gctggccgct ggcagtggcg ctactgggga aaagggagtt
     1441 ttacaccgat gaatgccacc tgggatgtcg caggaaaagg tgagtggcat gacagcacga
     1501 ttacgctgac cgatctctcc accggtttcg accagttaca atacggtacg atgacggtag
     1561 aaaagccgcg attaattctc gacaagcccg tcgtctgggg acgtgacgca cagcatccct
     1621 ccttcagcgg cgctctgtca ctggacgccg ggcaaacgct gttcactggc ggcagtgtgt
     1681 taccgccatc aaccttaaaa tttagtgtcg atgggcgcga tccgacgtat ttcctcttta
     1741 aaggcgattt acatgctggt gagattggcc cggttcgggt aaatggtcgc tgggacggta
     1801 ttcgtctgcg cggtaacgcc tggtggccta aacaatcact gactgtattc cagccgctgg
     1861 tgccacccga ctggaagatg aacttacgcg atggtgaact gtatgctcag gttgcatttt
     1921 cagctgcgcc tgaacaagga ttccgcgcgg gagggcacgg cgtgttgaaa ggcggtagtg
     1981 cctggatgcc agataatcag gttaacggtg tcgattttgt cctgcctttc cgttttgccg
     2041 atggagcctg gcatctgggg actcgcggcc ccgttacgtt gcgaattgcc gaagtgatta
     2101 atctggtgac agcgaaaaat attacggctg atttgcaagg tcgttatccg tggactgaag
     2161 aagaaccatt gctgttgact gatgttagcg tcgatgtgtt aggcggtaac gtgctgatga
     2221 aacaattacg tatgccgcaa catgacccgg cgctgttgcg gctgaataat ctttcctcca
     2281 gcgaactggt tagcgccgtc aatccgaaac aattcgccat gtccggggca tttagtggtg
     2341 cattgccgtt atggctaaac aacgaaaaat ggatagtgaa agacggctgg ctggcaaata
     2401 gcgggccgat gaccttgcgg ctggataaag acaccgcaga tgcggtggtg aaagacaata
     2461 tgaccgcggg ttcagcaatt aactggttgc gctatatgga aattagccgt tcatcgacaa
     2521 aaattaattt agataatctc ggtttattaa ccatgcaggc caatattaca ggtaccagtc
     2581 gcgttgatgg taaaagcggt acggtaaatc ttaattacca tcatgaagag aatattttta
     2641 cgctgtggcg cagtttacgc tttggcgata atttccaggc atggctggag cagaacgcgc
     2701 gtctaccggg aaatgactgt ccgcaaggaa aagagtgtga ggataaacaa tgaaaatttt
     2761 actggctgcg ttgacgtcat cttttatgct ggttggctgt acgcctcgca ttgaagtcgc
     2821 tgcacctaag gaaccgatca ctatcaatat gaacgttaaa attgagcatg agatcatcat
     2881 caaggcagac aaagatgtcg aagagctgct tgagacccgt agcgatcttt tttgaggtga
     2941 tgatgaaaag aacattactt ctttgtgcat ttcttgttgg cttggtaagc agcaatgtaa
     3001 tggcattgac tctggatgaa gccagaactc aggggcgggt aggggaaaca ttttatggtt
     3061 atctggttgc gctgaaaaca gatgctgaaa ctgagaaatt agtaaccgac atcaatgccg
     3121 aacgtaaagc gagctatcaa caactggcaa agcagaataa tgtgtcggtg gatgatatcg
     3181 cgaaactcgc cgggcaaaag cttgtagaac gggccaaacc cggggaatat gtacaaggga
     3241 taaacggtaa atgggtgcga aaattttaat agcaaaaccg caatatcggg ataacgctac
     3301 aggagaagac gattaatcta ttttccggaa acattttgct cttttaatta taaggatgag
     3361 ttatgtttcc gctctaattt atttatttga gtattgattt tatacccttt tttcattaaa
     3421 taaatatcta tgtgacgcat ttgaatttat aatttatata atatacataa tttgtatgtt
     3481 tcgcgggttt atttatttct ctgtattata aatccagatt gtcagagaat atgtttgatt
     3541 aggtaacaat ctactggcaa tattggacgt cctctatgtt ttaaataatc aattggtcgg
     3601 gtcagggtat ccgaccttct ttatattcgc cagaaggatt tattatgcaa aggaaaactc
     3661 tattgtcggc ctgtattgca ttagctctga gtggtcaggg ttgggcggca gatattacag
     3721 aaatagaaac caccacaggt gaaaagaaaa ataccaatgt gacttgtcca gcagatcctg
     3781 gaaaactcag tccagaagag cttaaacgct taccctctga atgctcctct gtagtcgaac
     3841 agaacctgat gccatggctt gtcacaggtg ctgctactgc gttaatcaca accttagcca
     3901 tagtggaact aaacgacgat gatgatcatc atcgcaataa ttctccactt ccaccaactc
     3961 cacctgatga tgattcagac gacactccag ttcctccaac tcctggcgga gatgagataa
     4021 taccagacga tggtccggat gataccccga cgcctcccaa accgatttcg tttaataatg
     4081 acgttatcct cgataaaaca gaaaaaacgt taactattcg cgattcagtt tttacttata
     4141 ccgagaatgc tgacgggact atttctctgc aagatagcaa tgggcgtaag gcgactatta
     4201 atctttggca gattgatgaa acgaataaca cggttgccct tgaagggatg agcgcagatg
     4261 gtgcaacgaa gtggcaatat aatcacaacg gtgagctcgt tattacgggc gacaatacca
     4321 cagtaaacaa cactggaaaa accatcgtcg acggcaaagg cgccaccgga actgaaatcg
     4381 cgggtaataa cgcggtggtg aaccaggacg gcgagctgga tgtgagcggc ggcggtcacg
     4441 gtattgatat caccggcgac agcgcgacgg tggacaacaa aggcggcatg actgtcaccg
     4501 atccggattc catcggtatt cagatcgacg gcgacaaggc ggttgtcaat aacgacggtg
     4561 acagtgccat cagtaacggt ggcaccggca cgcaggttaa cggtgatgaa gccaccgtaa
     4621 acaataacgg cagcaccacc gttgacggca aggattccac gggcaccgag ataaacggtg
     4681 acaaagccat cgtcaacaac gacggtgaca acacgatcct tgatggcggc accggaaccc
     4741 gcattacggg tgatgatgcg acggccaaca acagtggcaa taccaccgtt gacggacagg
     4801 gctcgaccgg taccgaaatt gcgggtaata acgcggtggt gaaccaggac ggcgagctgg
     4861 atgtgagcgg cggtggtcac ggtattgata tcaccggcga cagcgcaacg gtggataaca
     4921 aaggcggtat gaccgtcacc gatccggatt cgattggcat tcagatcgac ggcgacaagg
     4981 cggttgtcaa taacgacggt gacagtgccg tcagtaacgg cggcaccggc acgcaggtta
     5041 acggcgacga agccaccgta aacaataacg gcagcaccac cgttgacggc aaggattcca
     5101 cgggcaccga gataaacggt gacaaagcca tcgtcaacaa cgacggcgac agcacgatcc
     5161 ttgatggcgg caccggaacc cgcattacgg gtgatgatgc gacggccaac aacagtggca
     5221 ataccaccgt tgacggacag ggctcgaccg gtaccgaaat cgcgggtaat aacgcggtgg
     5281 tgaaccagga cggcgagctg gatgtgagcg gcggcggtca cggtattgat atcaccggcg
     5341 acagcgcgac ggtggacaac aaaggcggca tgaccgtcac cgatccggat tccatcggta
     5401 ttcagatcga cggcgacaag gcggttgtca ataacgacgg tgacaacgcc atcagtaacg
     5461 gcggcaccgg cacgcaggtt aacggtgatg aagccaccgt aaacaataac ggcagcacca
     5521 ccgtagacgg tcagggttcg accggaactg aaatcgcggg taataacgcg gtggtgaacc
     5581 aggacggcga gctggatgtg agcggcggcg gccacggtat tgatatcacc ggcgacagcg
     5641 cgacggtgga caacaaaggc ggcatgaccg tcaccgatcc ggattccatc ggtattcaga
     5701 tcgacggcga caaggcggtc gtgaacaacg acggtgacag tgccatcagc aacggcggca
     5761 ccggcacgca ggttaacggc gacgaagcca ccgtaaacaa taacggtaaa accaccgttg
     5821 atggtcaggg ctcgaccgga actgaaatcg cgggtaataa cgcggtggtg aaccaggacg
     5881 gcgagctgga tgtgagcggc ggtggtcacg gggttgatat caccggcgac agcgcaacgg
     5941 tggataacaa aggcggtatg accgttaccg atccggattc catcggtatt cagatcgacg
     6001 gcgacaaggc ggtcgtgaac aacgacggtg acagtgccat cagcaacggc ggcaccggca
     6061 cgcaggttaa cggtgatgaa gccaccgtga acaataacgg taaaaccacc gttgatggtc
     6121 agggctcgat cggtaccgaa atcgcgggta ataacgcggt ggtgaatcag gacggtacac
     6181 tggatgtgag cggcggcggt cacggtattg atatcaccgg cgacagcgcg acggtggaca
     6241 acaaaggcgg catgactgtc accgatccgg attccatcgg tattcagatc gacggcgaca
     6301 aggcggttgt caataacgac ggtgacagtg ccatcagtaa cggcggcacc ggcacgcagg
     6361 ttaacggcga cgaagccacc gtgaacaata acggtaaaac caccgttgac ggcaaggatt
     6421 ccacgggcac cgagataaac ggtgacaaag ccatcgtcaa caacgacggt gacagcacga
     6481 tccttgatgg cggcaccgga acccgcatta cgggtgatga tgcgacggcc aacaacagtg
     6541 gcaataccac cgttgacgga cagggctcga ccggtaccga aatcgcgggt aataacgcgg
     6601 tggtgaacca ggacggcgag ctggatgtga gcggcggcgg tcacggtatt gatatcactg
     6661 gtgatagcgc cactgtaatc aacaaaggca atataactgt cacggataaa gattcggttg
     6721 gtgtgctgat taacggtgat cgagcgacct ttgccaatac gggtcatatt gacgtcaata
     6781 attcagctac tgggtttagc atcgcaacca gtgagggagt tatctcatta actggcagta
     6841 tggatgtagg tgacatctca acaggaatgg tgctcagcgg agatggcaac agcgtgacgc
     6901 tggcagtaga agatcttaat gttactgggc agaaagcgat gggtgtcgac atttctggtg
     6961 atagtaacga cattgatatc gctggcaata tcttggttga taaagatcaa actgcagata
     7021 atgcggcaga ctatttcttc gagtcatctg tcggggtgaa tgtcacgggt aacaataata
     7081 cagtatctct aaatggcgaa ttaacagttg tctctgacag cgaattaacc tcaagatctc
     7141 atggcaaacg tgatgggagt caagaaaata tctccgggct gattgtgtct ggtgataata
     7201 atacaataag cctaaatggc ggtatacagt ttatcggtga gcaaaaaata ctggcggatg
     7261 gttcagatat tgcatctctg cgtaaaggat ttagtgatac ctccataatc agcgttgatg
     7321 ggaactcatc agtatatttg aatgggactt cgacaatagg tggcgacctt cctttaggct
     7381 atacaagcat tattcagtta aaaaataaag ctatcctgga aattggtcgt gatgctttgt
     7441 tgggtgtaaa ggatattaaa tcgtttaata actattacga gccagtaccg aagattataa
     7501 aggctactac aggttctaag gtcatcaata atggcaatat agatatccaa aatatcggtt
     7561 ttatttcagt gtcaggtgaa gatacgtcgg gcactaataa tggcaacatt acgctatcac
     7621 agtacgatta cggcaacatg atgtcaggta ctaatgctct gttaagtacc gatggaggca
     7681 gtgttgttaa caacggcacc atcatcggga aggtcatgga gcaaagtagc gttattaatc
     7741 gatttgagac gaccgacgtt acttatgatg agttgtttaa taatagcgtt acgggcataa
     7801 ccggaatgct ttctaaaacc ggtgctatgg gcattaataa tgtaaactat tgatatgtat
     7861 ggacgcggta gcgtgggtat gttggcggtt actcaagcga ttatagaaaa tgctggcacc
     7921 atcaaactgg actctctatg ggttgatgct aacgatacca ctgcgttacc cgataatatc
     7981 gctatcagta atgcccgtta ttatggtgca gggatggcgg taggttcaga tagctatggt
     8041 agcccggatg caaatgtcac agctattaac caattaggtg gtgtgattag tgtctataat
     8101 gctggggtcg gtatggccgc ttacggcggt agtaatatgg ttattaacca aggtacgatt
     8161 aacctggaga aaaatgaaaa ctatagcagt agtctgagtg caaatacgct agttggtatg
     8221 gcggtttatc aacacggcac tgctattaac gataaaactg gcgtgattaa tattaatgtc
     8281 gataccggcc aggcgtttta taacgacggt acagggacaa tcctgaatta tggtgagata
     8341 aaccttctcg gttctccaat ggacagtgca gatccccata tgggggctac cccagataat
     8401 ctggatctgc tgtcagcact aacaggcagc ggcgaaacgg atatgcgaac tgcttcaagt
     8461 ggtgggtttg tgacgaccaa agcgctggca aactacggca atgaaacgct aaatagtaat
     8521 gtgaccgcca aagcgtggct ttataaccag gataaggcca atctgacaat taacggagag
     8581 ctatccgttg ggcaagggct ggaaaatagc ggattactgg attctgacac cattagtgca
     8641 gcagcgaatg tctataaccg tgcatcgggt agcattatta ccgacctgtt aatgttaacg
     8701 agtggtaact cgttttttaa cgagggtaat ttcagcggat ctatagttgg caacagctat
     8761 aggcaaaatg tggttaatac cggaagtatg acagtcacgg ctgatggtac atcgctcatc
     8821 ggcggcagct ttgtgcttta taacgaagcg ggggcaatac tcagcaacag caacagcgcc
     8881 gtttcgggtg gagaaaatgc cattgttaac ataacccgca ccagcgattc actcgcgcaa
     8941 gttaaccgcg gcactattac cgcgaccaac ggctacagtg cgattaaaac ggcaagtacg
     9001 ggttctaaca gcaacggaaa atggatctgg aataccgata cgggtgtaat tagcggcgtg
     9061 aatcctaatg caccgttaat tgatcttggt cgcggctata actttgccaa cgcaggcact
     9121 attaatgtgc agggtgatgg tgcagtcgct atcagcgggg gaaccaccag ctatacggtt
     9181 caactggtga acagtgggac catcaatgtc ggtacggcgc agggtaaagc ggacggcact
     9241 aacggtactg gtttaattgg tatcaagggg aatggccgtg agacgacaat taataacgca
     9301 caaagcggtg taattaacgt ttacgcagat aactcctggg ctttcggagg acagactaaa
     9361 acgatcatca ataatggtga aatcaacctg ctgtgtgata tgggatgtga tatttatgca
     9421 ccgggcacca ccggtacact gaacgatcat aacagtacta ctgatattat tgttcctgcc
     9481 gcaacgagca cacctacgca ggggtcagta cccacagtac ctgcggactc cagtgcacag
     9541 caaaaactga ctaactacac tattggtacc aatagcgatg gcacctctgg tatgttgaaa
     9601 gccaataacc ttgtcatcag cgacaacgtc aaggtaaata ccggattcag tgcaggtact
     9661 gccgacacga ctgttgtgat taatgatgtt ttcaaaggtg aaaatatttc cggggctgag
     9721 aatatttctt cttccacggt aatgtggaat gctcagggca gcactgatgc aagcggcaac
     9781 gtagacgtca cgatgaccaa aaacgcctat acggatgttg tgacggattc ttcagttaat
     9841 aatgtcgctc aggtactgga tgcgggctat accaacaacg agttgtacag cagcctgaac
     9901 gtaggcacca cggcggaact gaacagtgcg ctgaaacagg tcagcggcag ccaggcaaca
     9961 acggtattta atgaagcacg agtgttaagt aaccgcttca gcatgttgtc cgatgcagca
    10021 ccggaagttg caaacggtct ggccttcaac gtggtagcga aaggcgaccc gcgtgctgaa
    10081 ctgggaaata atacacagta cgacatgatg gcgctgcgta aatccatgac gctgactgaa
    10141 catcagaatc tgagtctgga atacggtatt gcgcgtctgg atggtaatgg ttcagatacc
    10201 gtaggcgata atggtgtgac tggcggttac agccagttct tcggtctgaa acaccagatg
    10261 gcgtttgaca acggtatgag ctggaataac gccctgcgtt atgatgtgca taatctcgat
    10321 agcagtcgct cgatagctta cggcgacgtc aacaaaaccg ctgatgctaa tgtgaaacaa
    10381 cagtacctgg agttccgtag tgaaggggca aaaacctttg aactgcgtga aggtctgaat
    10441 gtgacgcctt atgcgggtgt gaaactgcgt catacgctgg aaggtggcta tcaggaacgc
    10501 aatgcgggtg actttaacct gagcatgaac agcggcagcg aaacggcggt ggacagcatc
    10561 gttgggctga aactggacta tgcagggaaa gaaggctgga gtgcgaacgc cacgctggaa
    10621 ggtgggccga atctgagcta tgtgaaaagt cagcgaactg cgagcgtttc gggcgcggga
    10681 agccagcgtt tcaacattga tgatggtcag aatggtggtg gtttcaacag cctggcgaca
    10741 atgggtgtga aatacagcag ccaggaaagt gcgttgcagg tagatgcatt ccactggaaa
    10801 gaagacggca tcagtgataa aggtgtgatg ctaaacttca agaaaacgtt ctaattttta
    10861 gcatgtaata cataaaccgc aacgctgata caggttgcgg tttttttatt gtcggatgcg
    10921 gtgcttgacg tgtcttaacc tgtctggggt ttcgtgtctt tcagggcaac agtacaactc
    10981 atcccgcaat cttgtttaca ccctcgcatt atttatctcc attcatttct atactgattt
    11041 ttcttaatcc gttttattac agggcagggt gcgatgagca gcaacacatt tactctcggt
    11101 acaaaatccg ttaaccgtct tggttatggc gcgatgcaac tggcaggtcc tggggttttt
    11161 ggccccccaa gagatcgcca cgtcgctata accgtgctgc gcgaggcgct ggcattgggc
    11221 gtcaatcata ttgataccag cgacttttat ggtccgcacg tcaccaatca gattatccgc
    11281 gaagcgcttt atccttactc tgacgacctg acaattgtca ctaaaattgg tgcgcggcgt
    11341 ggagaggacg cttcctggtt acccgcattt tctccggcag agctgcaaaa agcggtacac
    11401 gataatctac gtaatctcgg cttggacgta ctggatgtgg ttaacctgcg cgttatgatg
    11461 ggggatggtc atggcccgac ggaaggatcg attgaggcca gcctgacagt gctggcggag
    11521 atgcaacaac aaggcctggt aaaacatatt ggcctgagta acgtcacacc gacgcaggtt
    11581 gcagaggcgc gaaagattgc cgaaatcgtc tgcgtgcaga acgaatacaa cattgcccac
    11641 cgtgctgatg acgcaatgat tgatgctttg gctcgtgatg gcattgccta cgtgccattc
    11701 tttccgctcg ggggctttac gccgctgcaa tcctccacgc tttccgatgt tgctacaagc
    11761 cttaatgcga caccaatgca ggtggcgctg gcgtggctgt tacagcgttc accgaatatt
    11821 ttgctgattc ctggcacctc ttcggtgggg catctgcggg agaatatggc gtccgatgaa
    11881 ttagtgctaa cggatgacgt gctggcaacg ctggacagta ttgcgcaaaa ggcgggttaa
    11941 ataaaaaaac ctcccacttt cgtgggaggc atcataaatc ttatgcagca acaatactgt
    12001 cgatggctgc tttcgcgtca gactgcgctt tcgctgccat ttccggaccg tatgcgatcc
    12061 cttcggcgaa gacaaatttt acatcggtaa tgccgataaa gccgaggaac gtggacagat
    12121 acggcgtcac cagatccgtt gggccatctt tatggatccc accgcggctg gtgataacga
    12181 tggctttttt actggtgacc aggccttccg gaccgttctc ggtatagcgg aaagtaacgc
    12241 ctgcacgtgc aaccaggtca aaatagtttt tcaactgggt cgagatattg aagttataca
    12301 tcggtgccgc aataacgata acgtcgtggg ctttcagctc ggcaatcagc tcatcggaaa
    12361 gcgccagagc ttcctgctga cgcggagtca gcggcgcatc gctcggacgc agtgcaccaa
    12421 ccagttcgcc atccaggacc ggaatcggat ttgcagccag gtcacgcacg gtgatttcat
    12481 cagcggagtg cttttcgcgc cattgttcaa caaaataatc ggacaactga ttagactgag
    12541 agtaccctgc caggatgctg gatttaagaa ctaatacctt gctcatggtg tttccttata
    12601 gatatttgaa tgggcaatgc cccgttgctt gttgacactt tattcacaat cctgccacag
    12661 agatagcgca ataaatcgaa gcctatgttc gaatttattg aacaacgcat agaaagccgc
    12721 gatgtggtac tctatatcta tcatttaaaa gaaaattaat caggcaacct actgcccact
    12781 aacgttatga cagaacaaca aaaattgacc tttgctgact tgcagcagcg gctggattcg
    12841 ctgatgctgc gcgacagaca gcgtttttta cgccgtctgc acggtgtgaa gaaggttaaa
    12901 aatcctgatg cacaacaagc cattttccag gagatggcga aagagattga ccatgcggca
    12961 gggaaagtcc tgctgcgtga agcggcacga ccggagatta cttacccgga caatttgccg
    13021 gttagtcaga aaaagcagga cattctcgaa gcgattcgtg accaccaggt ggtgatcgtc
    13081 gccggggaaa cgggttccgg taaaacgact cagctaccga aaatctgtat ggagctgggg
    13141 cgcgggatta aagggctgat cggccatacc cagccgcgtc gcctggcggc aagaacggtg
    13201 gcgaaccgta ttgcggaaga gctgaaaacg gagccgggcg gttgcatcgg ttacaaagtg
    13261 cgtttcagcg atcacgtcag tgataacacg atggtcaagc tgatgaccga cggcatcctg
    13321 ctggcagaga tccagcaaga ccgcctgctg atgcagtacg acaccatcat tattgacgaa
    13381 gcgcacgaac gcagcctgaa tatcgatttt ctcctcggct atttgaaaga gttgctgccg
    13441 cggcgtcctg acctaaaaat tattatcact tccgcgacta tcgacccgga acgcttttcg
    13501 cgccacttta ataatgcgcc gattatcgaa gtctccggtc ggacctatcc ggtggaagtg
    13561 cgctatcgcc cgattgttga agaagccgat gataccgagc gcgatcagtt gcaggcgatt
    13621 tttgacgccg tagacgaact gagccaggaa agccctggcg acattctgat ctttatgagc
    13681 ggcgagagag aaattcgcga taccgccgat gcgctgaaca agctgaactt acgccatacc
    13741 gaaatcttgc cgctttatgc acggctttcc aacagcgaac agaaccgcgt gttccagtcg
    13801 cacagcgggc gacgcattgt actggcgacc aacgtcgcgg aaacctcgtt gaccgtaccg
    13861 ggtattaaat acgttatcga cctaggtacg gcgcgtatca gccgctacag ctatcgcacc
    13921 aaagtgcagc gtttgccgat tgagccaatt tcgcaggctt cagctaatca gcgtaaaggc
    13981 cgctgcggtc gtgtgtcaga agggatctgt attcgtctct attccgaaga cgatttcctc
    14041 tcgcgcccgg agtttaccga cccagaaatt ctgcgtacta acctggcctc ggttattttg
    14101 cagatgaccg cactgggctt gggcgatatc gccgcattcc cgtttgtcga agcaccggat
    14161 aaacgcaata tccaggatgg cgtgcgtctg ctcgaagagc tgggcgcgat caccactgat
    14221 gaacaggcca gcgcctataa actgacgccg ctcggtcgcc agctctcgca actgccagtc
    14281 gatcctcgtc tggcgcgaat ggtgctggaa gcgcagaaac atggctgcgt gcgtgaggcg
    14341 atgattatca cttccgcgct ctctattcag gacccgcgtg agcggccaat ggacaagcag
    14401 caggcatcgg acgaaaaaca tcgtcgcttc cacgacaaag agtctgactt cctcgcgttt
    14461 gtgaatctgt ggaattatct tggcgagcag caaaaggcgc tctcttcaaa cgccttccgt
    14521 cgcctgtgtc gtaccgatta tctcaactat ctgcgcgtgc gcgaatggca ggatatctac
    14581 acccagttgc gccaggtggt gaaagaactt ggcattccgg ttaacagcga accggcggat
    14641 tatcgcgaaa ttcacattgc gttgctgacc ggtttgcttt cgcatatcgg catgaaagat
    14701 gccgataaac aagaatatac cggcgcacgt aacgcgcgtt tctccatctt ccccggttct
    14761 ggtttattca aaaagccgcc gaaatgggta atggtggcgg aactggtaga aaccagccgc
    14821 ctgtgggggc gcattgcggc acgcatcgat ccagaatggg tggaacctgt cgctcagcat
    14881 ttaatcaaac gcacctacag cgaaccgcac tgggaacggg cgcagggcgc ggtgatggca
    14941 acggaaaaag tcaccgttta cgggttgccg attgttgctg cgcgtaaggt caactacagc
    15001 cagatcgatc cggcgttatg tcgtgaactc tttattcgcc acgcgctggt ggaaggtgac
    15061 tggcagacgc gtcacgcatt cttccgtgaa aacctgaaac tacgggccga agtggaagaa
    15121 ctggaacaca aaactcgtcg ccgcgatatt ctggtggatg acgaaacgct gtttgagttc
    15181 tacgaccagc gcatcagcca cgatgtgatt tccgcacgtc acttcgacag ctggtggaaa
    15241 aaagtcagcc gcgaaacgcc tgatttgctc aactttgaaa agagcatgtt gatcaaagaa
    15301 ggggcggaaa aaatcagcaa actggattac ccgaacttct ggcatcaggg caatctcaaa
    15361 ctgcgtttga gctatcagtt tgagcccggc gcggatgctg acggtgtgac cgtgcatatc
    15421 ccgctgccgt tacttaacca ggttgaggaa agtgggtttg agtggcagat ccccggtctg
    15481 cgtcgtgagc tgatcatcgc cctgattaaa tcgctgccga agccggtacg ccgtaatttt
    15541 gtgcccgcgc caaactacgc cgaagcgttt ttaggccgcg tcacaccgct ggaattgccg
    15601 ttgctcgaca gccttgaacg agagttacgg cggatgactg gcgtcaccgt tgaccgcgaa
    15661 gactggcact gggatcaggt gcccgatcac ctgaaaatca ctttccgcgt ggtggatgac
    15721 aaaaacaaga agctaaaaga agggcgctca ttacaggatc tgaaagacgc cctgaaaggc
    15781 aaagtgcagg aaacgctgtc tgccgtggcg gatgacggta tcgagcagag cggtttacat
    15841 atctggagtt ttggtcaact gccggaaagc tacgagcaga agcgcggcaa ctacaaagtg
    15901 aaggcgtggc cggcgctggt ggatgagcgt gacagcgtag cgatcaaact gtttgataac
    15961 ccgctggaac agaagcaggc aatgtggaac ggtcttcgcc gtttactgct gctaaatatt
    16021 ccatcgccga tcaagtactt gcacgaaaag ctgccaaaca aagccaagct ggggctgtac
    16081 tttaacccgt atggcaaagt gctggagctg atcgatgact gtatctcctg cggtgtggat
    16141 caactgatcg acgccaatgg tggcccggtc tggacggaag aaggctttgc tgcgctgcat
    16201 gaaaaagtcc gcgctgaact gaacgacacg gtggtggata ttgcgaagca ggtcgagcaa
    16261 atcctcactg cggtgttcaa tatcaacaaa cgtctgaaag ggcgggtgga tatgaccatg
    16321 gcgctggggc tttctgacat taaagcgcag atgggcgggc tggtgtatcg cggttttgtc
    16381 actggtaacg gcttcaaacg gctgggcgac acgctgcgtt atttgcaggc gattgaaaaa
    16441 cgactggaaa aactggcggt cgatccacat cgtgaccgtg ctcagatgct gaaagtcgaa
    16501 aacgtccagc aggcatggca gcaatggatc aacaaactgc cgcctgcacg tcgtgaggat
    16561 gaagacgtga aagaggtccg ctggatgata gaagagctgc gcgttagtta cttcgctcaa
    16621 caacttggta cgccttatcc gatttcggat aagcgtattt tgcaggcgat ggagcagatt
    16681 agcggttaac actgctattt gcctgataaa tctaa
//
LOCUS       DAZKHK010000112        16180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKHK010000112 DAZKHK010000000
VERSION     DAZKHK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.112"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(180..287)
                     /locus_tag="ACHOS5_003797"
     CDS             complement(180..287)
                     /locus_tag="ACHOS5_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9778545.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            763..2034
                     /locus_tag="ACHOS5_003798"
     CDS             763..2034
                     /locus_tag="ACHOS5_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP9778546.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            complement(2064..3068)
                     /gene="dppF"
                     /locus_tag="ACHOS5_003799"
     CDS             complement(2064..3068)
                     /gene="dppF"
                     /locus_tag="ACHOS5_003799"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP9778547.1"
                     /translation="MSTHEATPQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRQEKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(3065..4048)
                     /gene="dppD"
                     /locus_tag="ACHOS5_003800"
     CDS             complement(3065..4048)
                     /gene="dppD"
                     /locus_tag="ACHOS5_003800"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP9778548.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(4059..4961)
                     /gene="dppC"
                     /locus_tag="ACHOS5_003801"
     CDS             complement(4059..4961)
                     /gene="dppC"
                     /locus_tag="ACHOS5_003801"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP9778549.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4971..5990)
                     /gene="dppB"
                     /locus_tag="ACHOS5_003802"
     CDS             complement(4971..5990)
                     /gene="dppB"
                     /locus_tag="ACHOS5_003802"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP9778550.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6141..7748)
                     /gene="dppA"
                     /locus_tag="ACHOS5_003803"
     CDS             complement(6141..7748)
                     /gene="dppA"
                     /locus_tag="ACHOS5_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP9778551.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8492..8568)
                     /locus_tag="ACHOS5_003804"
     tRNA            complement(8492..8568)
                     /locus_tag="ACHOS5_003804"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8532..8534),aa:Pro,seq:cgg)
     gene            complement(8657..10348)
                     /gene="eptB"
                     /locus_tag="ACHOS5_003805"
     CDS             complement(8657..10348)
                     /gene="eptB"
                     /locus_tag="ACHOS5_003805"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP9778552.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEASAN"
     gene            complement(10601..11125)
                     /gene="lpfE"
                     /locus_tag="ACHOS5_003806"
     CDS             complement(10601..11125)
                     /gene="lpfE"
                     /locus_tag="ACHOS5_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP9778553.1"
                     /translation="MKRTILALCLLPFSFSSLSATTDLGAKGTLKFSLKISQGACELV
                     KDSVEVDMGLAVLKKPVRIGTELNPTTFSIGLKNCSEVVRAYVTMDGTPNADDPNLFA
                     LDAGGATGVGLKIKTSAGVQQFPKNTTPTPIEFAINGDGAHQLNYIASYVPLRADATI
                     GRADATVDFTVQYE"
     gene            complement(11131..12183)
                     /gene="lpfD"
                     /locus_tag="ACHOS5_003807"
     CDS             complement(11131..12183)
                     /gene="lpfD"
                     /locus_tag="ACHOS5_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFP9778554.1"
                     /translation="MKIKNLLAGMVLLGTSTFTGNIWAADWGPCRTASGDPFIFITSF
                     TKNIQNPTDNVTGQTYPDFYQWALGDKYSGVCECPSPNPTEARPTLYKTESTLAAGHN
                     STYFKITNNLEVSTRVYIANVGNVQVPFINKSNSQPGRECDQPTFGWTTGSKGQLSLY
                     IAKPFVGEQNIPQTIIVSVFGTKKENVYSSVPISQVLLSGKVTVTQGCELAAGTSLDI
                     DFGEYQAHDFKGRTGQPPQNVQKIQKELTFNCTNISDGVHIYLSLEGTPNAAYPSAIS
                     LGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEYVKRKVTTTITAYPVSTTGKLP
                     AAGDYSGVATMHVELD"
     gene            complement(12194..14725)
                     /gene="lpfC"
                     /locus_tag="ACHOS5_003808"
     CDS             complement(12194..14725)
                     /gene="lpfC"
                     /locus_tag="ACHOS5_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFP9778555.1"
                     /translation="MHWMHLPLDHYRAHFSFSLLALTIASALPAYGGKFNPKFLENVQ
                     GIDQHVDLSVYDSPVGQQIPGKYRVFVFVNEEKMASRTLDFSTASEAQRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKILPPEACVAFDEIIPQATSRFDFNTQTLHLTFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGSGSSPDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKESDGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSGKPRFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKDEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSIAYTWTRSPEWDED
                     DRLWSFSLSIPLGGAWGSYRMTTDQNGKTSQQASVSGTLLEDRNLNYNVQQGYTSNGV
                     GNSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAIVRSRFDTRVGYRVLMSLKRANGNAVPFGATAALSDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGHEQAQRCRVPFRLPEKKDNSGIVMVNAVCDK"
     gene            complement(14748..15434)
                     /gene="lpfB"
                     /locus_tag="ACHOS5_003809"
     CDS             complement(14748..15434)
                     /gene="lpfB"
                     /locus_tag="ACHOS5_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP9778556.1"
                     /translation="MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGV
                     AGEMLTKSIN"
     gene            complement(15518..16042)
                     /gene="lpfA"
                     /locus_tag="ACHOS5_003810"
     CDS             complement(15518..16042)
                     /gene="lpfA"
                     /locus_tag="ACHOS5_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFP9778557.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGSVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTTTGDKSAATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     ISVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVATKNAVTI
                     GYGNAEVDFNLTYN"
BASE COUNT         3983 a         4221 c         3853 g         4123 t
ORIGIN      
        1 tacgactgtt tttctgctcc ggaatccgtc acgtcttcac tctgtgctgc gcattccgca
       61 gaatattatc cgcttgcaac cgttgcattt ttcttacgaa aaaggccgca gagtttcacc
      121 tctgcggcct ggtccgggcg caaattgcca ttacggcagc ctgacgcccg catgacacgt
      181 tacttccgct tgttcagcca gttcacgatc atactggcaa gaatgccagc aatgaccgga
      241 gccgctaaat catgccagaa ggccatgccc agctctgcga gcgtcatata gccgcctgtg
      301 ttgtaatgac aacgtttcgc ggctattctt gaatggtcta gatgcaagaa tggcccccgt
      361 gatgttgtca ggtgcatacc tgcaacgtgc gggggttttc tctctccagc aaccaatgcc
      421 accagggatt aagcccccgc aacattgcgc ctcaccggaa cctttccggc ttgccgctga
      481 ttttacgatt gctttttgcc gtcagcaccc ggcaatcttc ctcattgcgt gatgccttcc
      541 agcttaattt ctttatttgc aatagttgca gctcatatcc gggctgactc cagactagta
      601 gtgaaatgcg caattttgta gaattgcgcc tcctcgcgac ctccccagtg aatctgttgc
      661 ctcatttttt acatactgcg tattcgactt ctccacctgt tgcgcaagag aaactgggtt
      721 tattcatttt tgcgaggccg acttctttct ggacaggact ttatgcagca caacacacta
      781 ccgaaacacg atcagaaatt gccgtttaca cgctacgact tcggctgggt tttattatgc
      841 ataggcatgg cgattggtgc cggaaccgtg ctgatgccag tacaaattgg cttgaaggga
      901 atttgggtat ttattaccgc agcgatcatt gcttatcctg ccacctgggt agtgcaggac
      961 atttatttaa aaaccctttc tgaaagcgat tcctgtaatg actataccga tattatcagt
     1021 cattacctgg ggaagaactg gggaattttc ctcggggtta tctacttttt gatgattatc
     1081 cacgggattt ttatctactc tctctccgtg gttttcgaca gcgcctcgta cctgaaaacc
     1141 ttcggtttaa ccgatgccga tctttcacaa tctctacttt ataaagtcgc cattttcgcc
     1201 gtactggtgg caattgcgtc tggtggtgaa cgattactgt ttaagatttc cgggccaatg
     1261 gtggtggtca aagtagggat tatcgtcgtg ttcggttttg cgatgatccc gcactggaat
     1321 ttcgccaata taaccgcctt cccgcaagcc tccgtctttt tccgcgatgt cctgcttacc
     1381 attccctttt gcttcttttc tgcggtattt attcaggtac ttaacccaat gaatattgcc
     1441 tatcgtaaac gggaagcgga taaagtactg gcaacccggc tcgcgctgcg tacccaccga
     1501 attagttata tcacgctcat cgcggtgata ctgttttttg ccttttcgtt taccttctca
     1561 attagccacg aagaagccgt ttctgccttt gaacaaaata tctcggcact ggcactggcc
     1621 gcgcaggtga tccctgggca tatcattcat atcacctcta cggtgctgaa tatctttgcc
     1681 gtactgaccg cattctttgg catttatctc ggtttccacg aggccattaa aggcattatt
     1741 ctcaatctgt taagccgaat tattgatacc aggaaaatta actcacgcat gctgactctg
     1801 gcgatctgcg cttttatcgt cattacgttg acgatttggg tttcgtttcg tgtatcggta
     1861 ctggtgttct ttcagttggg aagcccgtta tatggcattg tgtcgtgcct cattccgttt
     1921 ttcctgatct ataaagtctc acaactggaa aaacttcgcg ggtttaaagc ctggatgatt
     1981 ctgctgtacg gcattttgtt atgcttgtcg ccactgttga agttgattga gtaaaccgga
     2041 gcgcatggcc ccggttttgt gagttaacgc tgcggatttt catcctgatc aacggcaaaa
     2101 caggctacca gttgaccgcc gtagtctttt agctgcggct gcaactggat gcaggggccg
     2161 aagcgccgac gacagcgggc gttgaaggcg cagcctggcg gtggattcag cgggcttggc
     2221 agttcaccgg taagtttgat gcgttcgcga cgatcgtccg ggttcaggcg tggtgtcgcg
     2281 gagagcagcg cctgagtgta cgggtggcgc gggttattga agatttggtc cttcgtcccc
     2341 ttctccacgc agcggcccag gtacatcacc atcacttcat cagcaatgtg ctccaccacc
     2401 gacagatcgt gcgagataaa gacataagat agccccagtt cctgctgcaa atccatcatc
     2461 agattcagca cctgcgcacg cactgagacg tccagcgcgg aaaccggctc atcggcaatc
     2521 accacatccg ggtcgagcat cagacctcgg gcaatggcga tacgctgacg ctgaccgccg
     2581 gagaacatat gcggatagcg gtcgtaatgc tcggttttca ggccgacttt cgccatcatc
     2641 gacagggctt tttcctgacg ctgttctttg cttaagctgg tgttgatcag cagcggctct
     2701 tcgagaatct gcccaacttt tttacgcgga ttcagcgaac cgtaagggtt ctggaagaca
     2761 atctggattt tctgccgacg taacttctgc gcctgcggat cgtgcttgag cagatcctgc
     2821 ccctggtaat acagttcgcc gccggtgggc gtttcaatca tcgtcagcaa ccgacctagg
     2881 gtcgatttac cgcagccaga ttcacctact actgccagcg ttttgccacg ttcaaggtta
     2941 aacgaaacgc catccagcgc cttcaccaga cgttccggcg cgaacatgcc tttcttcacc
     3001 ggataatgtt ttttcaggtc gatagcctgc aacagcggtt gttgcggggt ggcctcatgc
     3061 gtactcatag tgtcggcctc ccggcatcat caagtgggta atggcatttg gactgacgcc
     3121 cgtcagcgag catattcagc gccggttctt cagcacggca tttgtcagtg gcgtaggggc
     3181 agcgcgggtt aagcaggcag ccgttcgggc ggtcgtactt gccgggaacg acacctggca
     3241 acgacgccag acgttctttg tcctgagcaa attccggcag cgcacgcagc aatgcctgag
     3301 tatacgggtg acgcggcgca tggaagatag cgtgcgcatc gcccgtttct actacctggc
     3361 ctgcatacat cacgataatt ttatgtgccg cttccgccac cagcgccagg tcatgggtaa
     3421 ttaacaccag cgccatgttc tctttctgct gtagctccag cagcagttcg ataatttgcg
     3481 cctgaatggt cacgtccagc gcagtggttg gttcatcggc aatcagcagt tttggccgac
     3541 aggcaatcgc catggcgatc atcacgcgct ggctcatgcc gccggaaagc tgatgcgggt
     3601 aaacatccag acgcgatgcc ggatcgggaa taccgaccag attcagcagg tcaatcgctc
     3661 gctgacggcg ggtacttttg ttgccgcctt gatgcacctt aatcgcttcc ataatctgga
     3721 aacccacggt gtagcacggg ttaaggctgg tcatcgggtc ctggaagatc attgccactt
     3781 cggcacccac caggttgcgg cgctcttttt ctgagatacg ctgcaaatcc tgaccgttaa
     3841 actccagctt ttccgccatt acgcggcccg gataatcaat cagccccata attgccagcg
     3901 aactgaccga tttaccagag ccggactcac ccacaatccc gaccacttca ccctgtttta
     3961 cgctgtagct gatgcggtct acggcgcgga acggtgcgct ttcgtcgccg aaatgcaccg
     4021 ataatttatc tacatttaat aacgccatct cgaacctctt actgcttcag tttgggatcg
     4081 agcgcgtcac gcagaccgtc acccatcagg ttaaatgcca gcaccgtcag caggatcgcc
     4141 agacccggga aggtcacgac ccaccaggcg ctttgcgcga actgcaacac atcagagagc
     4201 atggtgcccc actccggcgt tggcggctgc gcgcccatgc cgaggaaacc aagagcagcc
     4261 atatcgagaa tggcgttaga gaaaccgagc gacgcctgaa caatcagcgg cgcaaggcag
     4321 ttcgggaaga tgttaataaa catctggcgc attgccccgg cacccgccac gcgagacgcg
     4381 gtgacgtaat cgcggttaac ttccaccagc acggcggcgc gggttaagcg cacataatgc
     4441 ggcagcgcaa cgaaggttag cgccagcgcg gcgttaccaa tcgacgggcc gaaaatcgcc
     4501 accagcacca gtgccagcag tagacttggc agcgccagca tgatatcgac cacgcgcatg
     4561 atgatgttat cgaccagacc gccaaagtaa ccggcgatca ggccgagaat aacgcccata
     4621 atcagcgata acaccactac cagacagcca accagcagcg acaagcgcgc gccgtacatc
     4681 agccgagaca gcacatcacg ccccacgtca tcagtgccca gcaagtgcgc catgctgccg
     4741 ccttcctgcc aggctggcgg ggcgagcagt gcatcgcgga actgttccgc tgggttatag
     4801 ggtgcaatcc agttggcaaa gatcgcgata aacagcacga tgacgacgta aaccagcccg
     4861 acaaccgcgc ctttgttgcg tttaaaatag tgccagaact cctgtaacgg ggtcatcggc
     4921 accggtgcgc taatcacttt attttcagta acctgtgaca tgatgtcccc ttacttctta
     4981 tgacgaatac gcgggttcac cacgccgtac agcagatcga ccagcaggtt gacgaggata
     5041 atcatcgtcg ccaccagcaa tacgccgccc tgcactaccg gataatcgcg gcgttgcagt
     5101 gcgtcaatca accagcgccc cagaccgggc cacgagaaga tggtttcagt cagaatcgcc
     5161 cccgccagca atgttcccac ctgcaggccg ataacggtca ccaccggcag catcgcgtta
     5221 cgcagcgcat ggacgataat cacccgcatc cgggtaagac ctttggcacg cgcggtgcgg
     5281 atataatctt cgcccagcac ttccagcatc gaggagcgcg tcatacgcac aatgaccgcc
     5341 agcggaatag tacccagcac aatggcgggc aggatcatat gggcgacggc atcgataaag
     5401 ttaccgtctt caccccagat ggcggtgtcg atcagcataa aaccggttaa cggattggaa
     5461 tcatcgagga acaccatgtc gctcacgcga ccggagacag gcgtcaggtt ccagtgcacc
     5521 gaaaccagca tgatcagcat catgccccac cagaaaattg gcatcgagta gccggtcagc
     5581 gccaggccaa ccgctgtgtg atcgaaaatg gaaccacgtt taaccgcagc cagcacacca
     5641 accggaatcc cgactgcggt cgcaaaaatc atcgcgcaga cgccaagttc cagcgtggcc
     5701 tgaaagcgcg gcacgaactc ttcccatacc ggaattcggc ttttcatcga aatgcctaaa
     5761 tcaccgtgca ttacgcccca aatgtaatgg agatactgct gccacatcgg tttatctaag
     5821 ccgagttcag ccagcaattg cgcgtgacgc tctggggaga tcccacgttc gcccgccatg
     5881 atcatcaccg gatcgcccgg gatcatgtgg acaaaggcaa atgtgagaag ggtaataccg
     5941 ataaacgtgg ggatgacgag tcccaaacgt cggagaataa actgcaacat aacccggatt
     6001 ctctatagtg acccgcagcc tggaactgcg tgtctgtatt gctcacaaat caattcccgc
     6061 ccttgtggga cagggaatga agtattgctg ccggatggcg ctgtacgcag aggcacagcg
     6121 ccaccgggta tggcttttaa ttattcgata gagacgtttt cgaagtgatg tttgcctaat
     6181 ggatcaacca catagccttt aacttctttg cgtaccggtt caaacacggt ggagtgagcg
     6241 atgatcagtg ccggagcctg atcgtgcatc acgacctgtg cttgtttgta cagttcaaca
     6301 cgtttattgt ggtcgtcggt agcacgcgcc ggttgaatca gatcttcaaa cggtttgtag
     6361 caccattttg agtagttgga gccttgttca gaggcggcgc agctgaacag ggtggcgaag
     6421 aagttatccg gatccccgtt atcgccagtc cagcccatca ttaccgtctg atgctcgcca
     6481 tctttcgcac gcttgaggta ttcaccccat tcgtaggtga cgattttggc ctgcacgccc
     6541 actttcgccc agtctgcctg aatcatctcc gccatgcggc gagcattcgg gttatacgga
     6601 cgttgtaccg gcattgccca caggtcgatg gagaaacctt tttccagacc cgcttctttc
     6661 agcaaggctt tcgctttttc aggatcgtaa gtgtagtcct gaacgtcgtc gttatagccc
     6721 cacatggttg gcgggatcag gtttttcgct gatacgcccg cgccctgata aaccgctttg
     6781 atgatggcgt ctttgttcac cgcgtaggtc agagcctggc gaactttcac gtcatccagt
     6841 ggttttttct gcacgttata cgagagataa ccgacgttca gccccggcat ttccatcagg
     6901 ttgatggatt tatcctgctt catgcgggcg atatctgccg ggttcgggta cggcatcacc
     6961 tggcattcgt tcttctgcaa tttcgcgtaa cgcacggaag cgtcaggagt aatagagaag
     7021 accagcgtat cgatcttcgg tttggtgccc cagtagccat caaacgcttt atagcgaata
     7081 cgggaatctt tctggtactg ctgtaactgg aacgggccgg taccgattgg gttgaggtcc
     7141 agtttttccg gtgtaccggc tttcatcatc gcgtcagcat attcttttga cagaatagag
     7201 gcgaagtcca ttgccaggtc agcgaggaac ggcgcttccg ggcgagtcag cacaaactga
     7261 acggtgttat cgtccacctt tttcacttcg ctgatcagct ctggtaagcc catgccttcg
     7321 aagtattcgt agctgccgcc agagacttta tggtacgggt tttgcgcgtt tttctgacga
     7381 tcgaacgaga acactacgtc atcggcattc agttcgcgcg tcggtttgaa ttctttattg
     7441 tcgtgccact tcacaccttt acgcagatgg aaggtatagg ttttaccgtc ttcgctgact
     7501 tcccactttt cagcgaggcc cgggatcact tcagtggtgc cgattttaaa ttcaaccaga
     7561 cggttataaa gcggtacgga agaggcgtca taggtggtgc cggaggtaaa cagctgcggg
     7621 ttaaaccctt ccggagatcc ttctgagcaa taaaccagag ttttagcctg aacacttgct
     7681 gcgacggtca tagccaccag gctgagacca agcttcagca tccctgactt tttcaaggaa
     7741 atacgcatta ttctgctcca attgtgatgt ttgttgtttt aaccctttgc agtgggttgt
     7801 cgctgcctga ccttttttgt tttttgcccg gtcgggtcaa cgttatgagg tggggatgcc
     7861 gtactaatta acatcagtgt aacagtgcgg atcctccata aaatgcccct cgtgacctac
     7921 aatctgtcaa cagaatgtga aaacgtcaat acagccaacc gggatttaca ccaacggtga
     7981 gaatccacaa acaaagatta aaaaaacttc aaacagctat ttgcagcaga gaaatttgtg
     8041 ctacgccaac acaaccagaa caatcagctt aatatttagc aacatttggt gattaagttt
     8101 tatgcattta atgaaaaaaa tctgaggaaa aggtggatat ctgagcgatt aatactatga
     8161 acgctaaaac gcacagcgga aaatgccagc gccagccata agtaacgcaa aaaaagattt
     8221 aagcaaatat aaaaaaagac aatggtttct gtcacaaatc cgttaatgga tagtgagata
     8281 tggggcgcaa atttggcagg gaaatgtgtg gtgtgtagat cggataaggc ggtcgcgccg
     8341 catccgacaa tgggccggta catttgcctg atgcgacgct ggcgcgtctt atcaggccta
     8401 cgtcgagtcg aacctccccg cccccggagg ttctcttcct catgagctac agatgcaaaa
     8461 aagcctgctc gttgagcagg cttttcgaat ttggtcggtg atagaggatt cgaacctccg
     8521 accccttcgt cccgaacgaa gtgcgctacc aggctgcgcc aatcaccgaa tgcggggcgc
     8581 atcttactgc gcagatacgc cctcgtcaat cccttaatag caaaatgcct tttgatcggc
     8641 gagaaagtca gcaggcctag ttagccgacg cctcttttgc ctgcgggatg tgacaccagt
     8701 tgttgttttc gttaattcca ccatccggtg aagtatagcc aagacaaccc atgatggtgt
     8761 cgtacagctc tacatgacgg cgtggcactt tcatgtcggc ttcttttttc agttgcgcaa
     8821 atgcctgcgc attgaccgga ttttccagat atttatccga catccagacc atcatcggca
     8881 cacggaactg ttccggcggt gccagttcac gcggcgtgcc gtgcaggtgc tcgcgttcat
     8941 taattgactc accgtggtca gctgcataga acacaatcgc tttcttatcg cgaacctgat
     9001 caatcacgct ggaaataaag tgatccacat aggtcaccga gttgtcgtat gagttgatca
     9061 tctgcgcttt ggtacagccg ctatcaacac caatacattc cggcttccac tgcgcaaagc
     9121 tgcgcgggta acgctgggtg tagttaaagt gtgagccttt ggtatgcaga atgatcagat
     9181 gcttaccatc cgggttgcgc cccagcgatt gctgcatttc gtctaccagc aacatatcat
     9241 ctaccggctt gccacggtta cgtggctccg caccaatctg ctcacgataa gcaatgttgt
     9301 ccgccatcgt attgctgtag aaccacattt cgctttgcat agcgtagaga tcagaactga
     9361 atcctaactg cttcaatacc gcgaaaatgt tctgttcttt taatgtgcgc tgcggattat
     9421 cttctgctcc accctgacgt acaaacatgc aacgcagtga gagtttggtt gcagtatcac
     9481 aggagtaacc acggaacgcc gccagatttt tctcctgagc cagcttcggt gtggtattgc
     9541 gttcatagcc gaaaatgccc atatgatccc agcgcgtggt ttcaccaata atgaaaacga
     9601 catatgtatc atcaacgttt tgtggagcct ggtacgtaaa cttcttcgca ggattaagga
     9661 gagaattgtt atcggaagat tcatcgaccc gcgcccaggc ataaagacct aatgccgaaa
     9721 gccagtttga tggcagataa gagttcgcga cgacgccgcc ataactcggc aaatcaacac
     9781 ctgtcgccct ctccactttc ttctgctgga tatccagcaa acgaatcggt gcccaaacca
     9841 taatacctgc cagtacgacg accgccaggc tgcgaatacg ttgccctggg gttcgcagtt
     9901 gtcggagcaa ggtgtaacga cagcggttat tccagataag gataagaggc aacgcgctta
     9961 cggcgattaa ccagagaata aagttcagac caacaacttc ttttgacagg tcgatatcgg
    10021 tggtcatgac ggatgcgatg atgccataac caatgactac attaaggaag gtcatgtaat
    10081 agctggcacc tgcggaaaag agcaccacca gcgatgccag aatacgccag ctgcggcggc
    10141 caaacagcga aagaagacgc agtaaaaaga aggtcaccag tacggtggcg gccagttcaa
    10201 caacagcaga aatacctttc cagacggtaa aatcgtgcgc atagctgccg aagcggcggt
    10261 aaaaaaccgc gccattcata aaaaggccaa tatagattgc aagcaagaag ctcagcttct
    10321 gctgtgtaat cgatttgatg tatctcatgc aaacaaaccc tggacaaaca ggtgataaca
    10381 ccctgcttag tggcttttca ggccaggcag gggaaaagtg cggaggtagt cagataagta
    10441 aaatctacac gcgcgaataa gtagaccaca gcaaagggaa aaagtgtggg gaaagagtgt
    10501 gcacgaagca caaatttaca aaaatgcaag tttgaaaaga gactttagcg ttacaaaaag
    10561 cagtaaaaag ggccagtgag ctagccccgg tgaagcgaaa ttattcgtac tgaaccgtaa
    10621 aatcgaccgt cgcatctgcg cggccaatgg ttgcatcagc acgcagcggc acgtagctgg
    10681 cgatatagtt cagttgatgc gcgccatcac cgttaatcgc gaactcaatc ggcgtcggtg
    10741 tcgtattttt cgggaactgt tgtactcccg cagaggtttt aattttcaac cccacgcccg
    10801 tagcaccacc cgcatccagc gcaaaaagat ttggatcgtc cgcattgggt gttccatcca
    10861 tggtgacgta agccctgaca acttcgctac agtttttcag accgatggaa aaagtagtgg
    10921 gattaagttc tgttccgatt cttactggct ttttgagaac cgctaatccc atgtcaactt
    10981 caacactgtc ttttaccagt tcacaggctc cctggctgat tttaagagag aatttcaacg
    11041 ttcctttcgc ccctaagtct gtggttgccg acagtgacga aaaggaaaaa ggtagcaggc
    11101 ataatgcaag tattgttcgt ttcatcagtg ctaatccaat tcaacatgca ttgttgccac
    11161 accgctataa tcaccggctg cgggtaattt cccggttgta ctaaccggat aagcagtaat
    11221 ggtggttgtt actttacgtt tgacatattc gtacagtgag ccgggattca tttctaataa
    11281 gctattacta tcattcggct taagaatatt acctttacca tcctcaatta ccgcaccaac
    11341 atccgcgttg cccaaactga ttgcgctggg gtaagccgcg tttggcgtac cttcaagact
    11401 cagatagata tggacaccat cagaaatatt agtacagtta aacgttaatt ctttttgtat
    11461 tttctggaca ttttgtggcg gctgcccggt tcgacctttg aaatcatggg cctgatactc
    11521 accaaagtcg atatccaatg atgtccctgc ggcaagttca cagccttgag ttacggtgac
    11581 ctttcctgaa agaagcacct gtgatattgg aactgaacta taaacattct ccttttttgt
    11641 accaaacaca gaaactataa tagtttgcgg aatgttttgt tccccaacaa aaggtttagc
    11701 gatataaagt gagagctgtc ctttactccc tgtagtccag ccaaaagttg gttgatcgca
    11761 ctctcttcct ggctggctat ttgatttatt gataaatgga acctgtacat tccccacgtt
    11821 tgcaatatat acccgggttg aaacctcaag attattggta attttaaaat aggttgagtt
    11881 atgccccgcc gccaatgttg actcagtttt atatagcgtt ggtcgtgctt ctgtcgggtt
    11941 cggtgatgga cattcacaaa caccagaata tttatcaccc agtgcccatt ggtaaaaatc
    12001 agggtaggtc tgcccggtaa cattatccgt cggattttga atatttttgg tgaatgatgt
    12061 aataaaaata aaggggtcac cacttgccgt cctgcacggc ccccaatccg cagcccatat
    12121 attcccggta aaggtgctcg tccctaaaag caccatacct gccaacaagt ttttaatttt
    12181 catctgcata tccttattta tcgcataccg cattaaccat cacgataccc gaattatcct
    12241 ttttctctgg caagcggaat ggcacgcggc aacgttgcgc ctgctcgtgc ccccagctca
    12301 cctgcaattc gccctcttct ggcatcccgc tgatataaag ctgtccttct tcacccacaa
    12361 tgctgctggc aggtttgctc tcatcactta acgctgcggt agcaccaaac ggtactgcgt
    12421 tgccattcgc ccgtttcaaa ctcatcagca cacgataacc gacacgcgta tcaaagcggg
    12481 aacggactat cgccccacgc gttggaacga ctttctgaaa cgcttcctgt aattcaacgt
    12541 tttgccctaa cgtgtcgctg cgcaattcca caatggtttc acgatatggc gtgagatacg
    12601 gcaccactgc gttgcccatc cagtcaactt caacgccgct gctatttact acatgtccac
    12661 cacgcgcacc cggcgcagaa acaattgcca gactttcacc caagggttgc gagagagtga
    12721 taccttcact gtgaacaatg actccaccac gcacaccata gttaacttgt tggttatctt
    12781 tggaatagtt ataacccacg tcaatgttgc cagaacctcc catataaccc atgttgacac
    12841 taccactgtt gccaacccca ttactggtat aaccctgctg aacgttatag ttcaagttac
    12901 gatcttccag taatgtgccg ctgacagagg cctgttgtga cgtcttgccg ttctggtcgg
    12961 tggtcatacg ataactgccc catgcgccac ccagcggtat tgagaggcta aatgaccaca
    13021 gtcgatcgtc ttcatcccat tcagggcttc tcgtccaggt ataggcgata ctgtaattca
    13081 cacgaccaat atgaccgtga taaccagcgc ttaaggagtt ctccttacca tcaacgttcc
    13141 agtaatcctg ctgcgtcata ttgaagtaaa cagaaccgta gtctcccagt tgctgagtga
    13201 tgttaccttg tatctggctg cgcttatgat aacgccgata gtcactgtcg gcgtcactac
    13261 ggacatcggt cgcttcctgg aaggtgtaat aaccgctggt tgagtacttg tagcccaaca
    13321 gacgcaggtc ggtaccgccc tcaaaacttt tcgaatagag gaaacgataa gactgccctt
    13381 catctttttc attgttgcgt aactgacttt tcgcctgggt gacatccacc gataccgcac
    13441 caatggtgcc gaagtttttc cccagcccca gagctaaagc gttgtagtct gccgatagca
    13501 acgcaccacc ataggcggtc attccccacg gcaagccgta catggcagta aattgcccga
    13561 atcgcggttt accgctgtcg taattaccgg cacgatactc accggcagca aggctgtatt
    13621 tcagataccc ttcacgctgg aaaatcgcca ctgccgaaaa tggttgaata aaacgttgtt
    13681 cacttccgtc agattcttta atgatcaccg tcaagtcacc gctgccggag gttgcgtaga
    13741 gatcgttaat ctcaaatgcg cccggctgaa caaaggtacg ataaataaca tatccgttct
    13801 gttcgatact cacttcagcg ttacttttgg cgatgccacg caccaccgga gcaaagccac
    13861 gctggctatc cggcaacatt tcatcgtcgg atgccaataa tgccccgcgc atttgtacgc
    13921 tatcaaaaat ctcgccaggc gtggcagtat cacccaatgt tatctgtgac ttgagtggaa
    13981 taatcgcccg gtttaacgat gtaccaacgt tatcccattg atttttgcca tcggtacggt
    14041 tccagatact gttattacgt aaacgccacg gccccacgtt caaaccgctg cgcaggttca
    14101 ggtaataact gtcactggtc gaatctggag atgaaccgga accttcgttg cgaccattgc
    14161 tgccactaaa gctgtagtcc agtaacagcg caggtattcc ttcgtcccag cgagaaggat
    14221 caaccgtgcc acgagcggtc atcatcattg ctgcctgtgg aaaggtaaga tgcaacgtct
    14281 gagtattaaa atcaaaacga ctggtggcct ggggaatgat ttcatcaaac gctacgcagg
    14341 cttccggtgg caatatcttc agtgccggaa agctgtcgac gcgcacgccc atttcttcca
    14401 gttgaacacg gctaaggcaa ggcataagcg actcgccgct ggctttgcgt tgtgcttcgc
    14461 tggcagtact aaaatccagc gtccgggaag ccattttttc ttcgtttaca aacacaaata
    14521 cccgatattt accgggtatt tgctggccga caggagagtc gtaaactgac aaatcgacgt
    14581 gttgatcaat accctgaaca ttttcaagaa atttagggtt gaattttccg ccgtacgcag
    14641 gtaacgcgct ggcaatagtt aatgccaaca gtgagaaaga aaaatgcgct ctgtagtgat
    14701 caagaggtaa atgcatccaa tgcatagtaa agcgccttgt aaaaagatca gttaatcgac
    14761 ttcgtcagca tttcaccggc gacaccgaaa tcattaataa tgctgtattt cacttcgttc
    14821 gcattgcctg taccggctgg aaggttaatg tttaacgttc ctttagcgag gaccattccc
    14881 gcccgttcaa tcttttgtcc attaatggac atctgattaa aggtcaggtt ataagctgag
    14941 tcgttattaa ccacaatttg gttattttga cgctgaaatc gtaactgttt cattgcttca
    15001 aggttgcttc ccggcaagcc tttcggacgg tagaacaact tcaaacgggt gcgaacggct
    15061 atctgtaaga cgtttttatt ttcattttca gcacttttcg caggaatcgc ttttacatta
    15121 atccagtaaa cggattcacg atctttgggt aaggtatctt caatattaac aatacgtaag
    15181 acgttatttt tatcaggatc aagtttgaat aatggcggtg taataataaa cggcgttttt
    15241 ttatttccat tcacatcatc aatccatgac tggatcaaaa actcttcatt tttattatgg
    15301 ttttcaacgg tcagcgacgc ttctttcttg ccaccatcat aaataacgcg cgtcgtgctt
    15361 agggctacgc cgccagcaaa acactgttgt gcggtcatta aaagcgccag aacggcaagg
    15421 ctaattcgtt tcatgattcg gtccctgagt cattggtgtg aataatggca acagataatc
    15481 tgttgccatt gagagataat cgttccgtct ggatcgatta gttgtaagtc aggttgaagt
    15541 cgacttcggc attaccgtaa ccgatggtta ctgcattttt ggtagcgaca tagttagcgg
    15601 taaaatacag aacagtctgg ccttctttca ggacggtagc agatttaccg gtattcagat
    15661 caaccagcgt attggtatta tcataaatac caatacccac gccagttgcg gcacctggtt
    15721 cagtgcttac agaaatcagt ttgctgttgt ctgcgtcgcc aacaccgtta aagctgatct
    15781 ccacattttt gaaagtggag atatcgcagt tttccagttt gattttgaac ggagttgctg
    15841 cggatttatc accagtagtg gtaaacacag acttgttcac ctggcccaga acaacttctt
    15901 gtttttgagt gtctggagac acaacacaag cggcgtcaac aatctctccg gtgaatttaa
    15961 cagaaccgtc gcctgcatca gctgcgaata cggaagtaga ggtcagcgca agcgcagata
    16021 aggcaaacag aacctttttc atttataact ccatttatat agataaatat gcttgatgtt
    16081 gttcatacaa cgggtcgcag attatacaaa tggcgttaaa aaataaaatt atttttactg
    16141 aatattaatt tttgcaataa gttgaaatat gcttcaactt
//
LOCUS       DAZKHK010000113        16176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKHK010000113 DAZKHK010000000
VERSION     DAZKHK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.113"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..436)
                     /gene="rnpB"
                     /locus_tag="ACHOS5_003811"
     ncRNA           complement(60..436)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOS5_003811"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(469..1614)
                     /gene="garK"
                     /locus_tag="ACHOS5_003812"
     CDS             complement(469..1614)
                     /gene="garK"
                     /locus_tag="ACHOS5_003812"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP9778558.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1711..2601)
                     /gene="garR"
                     /locus_tag="ACHOS5_003813"
     CDS             complement(1711..2601)
                     /gene="garR"
                     /locus_tag="ACHOS5_003813"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9778559.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2631..3401)
                     /gene="garL"
                     /locus_tag="ACHOS5_003814"
     CDS             complement(2631..3401)
                     /gene="garL"
                     /locus_tag="ACHOS5_003814"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP9778560.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3417..4751)
                     /gene="garP"
                     /locus_tag="ACHOS5_003815"
     CDS             complement(3417..4751)
                     /gene="garP"
                     /locus_tag="ACHOS5_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP9778561.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5126..6697
                     /gene="garD"
                     /locus_tag="ACHOS5_003816"
     CDS             5126..6697
                     /gene="garD"
                     /locus_tag="ACHOS5_003816"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP9778562.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6846..7181
                     /gene="prlF"
                     /locus_tag="ACHOS5_003817"
     CDS             6846..7181
                     /gene="prlF"
                     /locus_tag="ACHOS5_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP9778563.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7181..7645
                     /gene="yhaV"
                     /locus_tag="ACHOS5_003818"
     CDS             7181..7645
                     /gene="yhaV"
                     /locus_tag="ACHOS5_003818"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP9778564.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7700..8509)
                     /gene="agaR"
                     /locus_tag="ACHOS5_003819"
     CDS             complement(7700..8509)
                     /gene="agaR"
                     /locus_tag="ACHOS5_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP9778565.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8758..10038
                     /gene="kbaZ"
                     /locus_tag="ACHOS5_003820"
     CDS             8758..10038
                     /gene="kbaZ"
                     /locus_tag="ACHOS5_003820"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP9778566.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVVERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10061..10534
                     /gene="agaV"
                     /locus_tag="ACHOS5_003821"
     CDS             10061..10534
                     /gene="agaV"
                     /locus_tag="ACHOS5_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP9778567.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10545..11324
                     /gene="agaW"
                     /locus_tag="ACHOS5_003822"
     CDS             10545..11324
                     /gene="agaW"
                     /locus_tag="ACHOS5_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP9778568.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11314..12192
                     /gene="agaE"
                     /locus_tag="ACHOS5_003823"
     CDS             11314..12192
                     /gene="agaE"
                     /locus_tag="ACHOS5_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP9778569.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFCLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12210..12644
                     /gene="agaF"
                     /locus_tag="ACHOS5_003824"
     CDS             12210..12644
                     /gene="agaF"
                     /locus_tag="ACHOS5_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP9778570.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12641..13774
                     /gene="nagA"
                     /locus_tag="ACHOS5_003825"
     CDS             12641..13774
                     /gene="nagA"
                     /locus_tag="ACHOS5_003825"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9778571.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDV
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14124..15278
                     /locus_tag="ACHOS5_003826"
     CDS             14124..15278
                     /locus_tag="ACHOS5_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP9778572.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVKLANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFMMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15291..16151
                     /gene="kbaY"
                     /locus_tag="ACHOS5_003827"
     CDS             15291..16151
                     /gene="kbaY"
                     /locus_tag="ACHOS5_003827"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP9778573.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         3887 a         4307 c         4154 g         3828 t
ORIGIN      
        1 ttcatcttac cctgggcacc aaaagcaaaa acccgccgaa gcgggttttt acgtaaatca
       61 ggtgaaactg accgataagc cgggttctgt cgtggacagt cattcatcta ggccagcaat
      121 cgctcactgg ctcaagcagc ctacccgggt tcagtacggg ccgtacctta tgaaccccta
      181 tttggccttg ctccgggtgg agtttaccgt gccacggact gttaccagcc gcgcggtgcg
      241 ctcttaccgc accctttcac ccttacctga tcccgcttac gcgggccatc ggcggtttgc
      301 tctctgttgc actggtcgtg ggtttccccc ccaggcgtta cctggcaccc tgccctatgg
      361 agcccggact ttcctcccct ccgcccgtct cccccgaaga ggacgacgac gaagcggcga
      421 ctgtctggtc agcttcggcg cgcagtatag agggtttgcg cgcccttgtc accccgcgtt
      481 gcgcattcca atcgccagtg tcgcggcgat attacgtgaa gcacggcaga tattgtcata
      541 agctccgcgg aatgcttcgt ctaacgtacc aatgctggtc agtacgctga agaccgcatc
      601 aatgccatgc tgatgtacaa cgccaacatc attggtcagg ctacccgcaa tgccaatcac
      661 cggtttatgg tacttcttcg ccacgtttgc gacaccaatc ggcaccttgc cgtgaatact
      721 ctggctatca atacgccctt caccagtgat caccagcgta caatcgtgaa tatgttcctc
      781 cagattcagc gccgtcgtga cgatttcaat accacttttc agttctgcac caagaaacgc
      841 catcagcgcc gcgcccatac cgcccgcagc tcctgcgccg gggacatctt tcacatcaac
      901 atgcagcgct tttttaatga catcggcata gtgagagagg ttattgtcca gctcgacaat
      961 catcgcctca ctggctccct tttgtgggcc aaagatgcgc gaagcaccac tgtcgcctac
     1021 taacgggttg gtgacatcac aagcgacgcg gatgacacaa tcttttaagc gcggatcgag
     1081 accggaaata tcaatatcat tcagagtatt aagactaccg ccgccaaaac caatttcatt
     1141 gccgttggca tcgcataatt tcgcccccag tgcctgtacc atgcctgcgc cgccgtcgtt
     1201 ggtggcgctg ccgccaatgc cgataataat gtttgtcgca ccgctctcca gcgcctgcag
     1261 gattaactca ccagtaccac gtgaagtggt cacgagtgga tcgcgttttt ccgcaggtac
     1321 cagttccagc ccactggccg ccgccatttc gataaacgcg gttttgccat cgccggagat
     1381 cccccaactg gcattcactt tctcgcccag cggccctgta acccaggcgt gacgttcagc
     1441 cccctgggtg gctgcaatca tcgcttccac cgttccttca ccaccgtcgg caaccggaac
     1501 agaaacatac tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac
     1561 ctcgctggca gataaacttt ctttataaga gtctggggcg attacgattt tcatacctat
     1621 gcctgttacc acgtgacgcc ggagggcgtt tctcttattc ggcctggatt ccaggcccgg
     1681 attgcaatac gccatccggg cacggcgtca ttagcgagta acttcgactt tcgccagttt
     1741 ttcgtagtag cacgccaggg cgctatgatc cgccgttcct aaaccatctg cacgcagtgc
     1801 ctgcatcatc tccataaccg cagctgtgag cggcagttgc gcgccgacgc cgtgagaagt
     1861 atccagcgca ttcgccagat ccttaatatg cagatcaata cggaagcccg gtttgaagtt
     1921 gcggtccatc accatcggcg ctttggcatc cagcacggta ctgcccgcca gcccaccgcg
     1981 aattgcctga tacaccagat ccgggttaac gcccgctttg gttgccaacg ttaacgcttc
     2041 tgacatcgca gcaatattca gcgccacaat gacctgattt gccagtttgg tgacgttacc
     2101 tgcaccgatt tccccggtat gcaccacgga acccgccatc gctttcatca aatcatagta
     2161 tttgtcgaaa atagccttgt cgccgcccac catcactgac agcgtaccgt cgatggcttt
     2221 cggttcaccg ccgctcaccg gagcatccag catatcaatg cctttcgctt tcagcgcttc
     2281 gctgatttca cggcttgcca gcggtgcgat agaactcata tcgatcaata ccgtacctgg
     2341 cttcgcgcct tcaataatgc cattctcacc cagcgccacc tctttcacat gaggggagtt
     2401 tggcagcatg gttatgatta cgtcgcactg ttcagcgatc gctttagccg tagaggctgt
     2461 ttctgcacct gcagcaatca cgtcagcgat agcttctggg ttacggtcag caaccaccag
     2521 cgagtaacct gctttcaaaa ggtttttact cattggttta cccataatcc caaggccaat
     2581 aaaaccaact ttcatagtca tatcaatcat ctctcttatt gcggtggtgg ttatttttta
     2641 aaggtatcag ccagtttctg agtggcagag cggaagacgc cgagatcgct gccgacggcc
     2701 acaaacgtcg cgccccattc cagataacga cgcgcatcag cctcgactgg tgcgaggatg
     2761 ccgctgggtt tgccgtgcgc gctggcacgg ttaaaaatgt gctgaattgc tttttgtaca
     2821 tccgggtgcg aggcattgcc gagatggcct aatgccgcag ccagatcgct ggggccgacg
     2881 aagatgccgt ctacaccttc ggtagcggca atagcatcga cgttatctac gccctgctga
     2941 ctttctatct ggaccagaat agtgatgttc ttgttcgact gagcgaaata atccgccacg
     3001 gtgccaaaca tattggcgcg gtgagaaacg gagacgccgc gaatgccttc cggtgggtaa
     3061 cgagttgatg ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag
     3121 ttatagaaac cgatatccag aagacgctta ataattaccg gctcgttggt cggcactcgc
     3181 actactggcg cgctggcgct gcctttcagg gccattaact gcggaataaa cgtggagata
     3241 tcgtttggcg catgttcacc atccagcacc agccagtcaa acccagccaa accaagaact
     3301 tcagtgctaa tcgggttaga gagagctgac cagcaaccaa tttgtacctg tttcgcagcc
     3361 agtgcggctt tgaatttatt cgggaaaaca tcgttattca tcgcttatac ctttgcttat
     3421 ttctgcaatt ccatacgttt aatgtcgcca actacgaaga ggtagcagac catcgccatc
     3481 agcgctgaac atcccacgaa aaccagtgct gcattgaagg agtgcagttc acttaccagg
     3541 tagccaatca ccagtggagt gacaatggag gcaacattgc caaagacgtt aaagacgccg
     3601 ccgcagaggc caacaatctc tttcggcgcg gtgtcagaaa tcactggcca gcccagcgca
     3661 ccaaatcctt taccaaagaa agccagcgcc atcagcatga ccaccagcgt ggtgttgttg
     3721 gtgtagttac ataagatgat ggtggaagcc agcaacattc ccagcacaat tggtagctta
     3781 cgagccaggg tcagggacaa accgcgtttg atcagataat ccgagaagac acctcccagc
     3841 acgccgcccg caaagccaca cagtgccgga atcgaggcga ccagacccac tttcagaatc
     3901 gacatgcctt tttcctgcac cagataaatt gggaaccagg tgaggaagaa ccaggtgatg
     3961 gtgttgataa aatattgtcc gaaaaatacg cccagcatca tgcggttaga gagcaattgc
     4021 ttgatgtaat gcagtttggg tccgcttgct gccgcactgc ccggcttttt gtggtccata
     4081 tcgaccaccg cgccattttc cgagataaac ttcagctctt ccgcagacat acgtgggtga
     4141 tctgtcggat tatgaatcaa cttgatccac agcgccgtca gcacaaaacc aatcaccccc
     4201 ataacggtaa agacgtgctc ccagccccag gcgaaagtca gccagccaag cagcggcgaa
     4261 aagagcgcca acgagaaata ttgcgccgag ttaaagatgg cggaggcagt accacgttct
     4321 ttcgtcggga accaggcggc gacaatacgg gcgttcgccg ggaatgatgg cgcttccgag
     4381 aaccccagca taaatcgcat aaagaacatg gagatcccag cccaggccag cggaaacata
     4441 tcgacaaagc cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta agtgtaaact
     4501 ttcttcgagc caaacttatc aagcagccag ccgccgggaa tttgcatcag caagtaggcc
     4561 cagccaaaag cagagaagat gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc
     4621 acttcggtac cagcaataga cagcgttgca cgatcggcgt agttaacggc ggtaacaata
     4681 aaaataatca gtaatattaa atagcgggta tgcacgcctt tctttttttc gtcaacggtg
     4741 tccagcatca ttttatttac ctcgggtact tatgctgatt tttattatta tggggaaggt
     4801 gttatttatg agtttcattt atgccgttac gacaatgaac tcgggaatta gtataagcag
     4861 cgcgagaata ataatcattg tgcaaatgct aatttaatta gtattattta aatattattt
     4921 tgagcatatg cacataaggt tgcgcgctaa agcacagatt tgcgctttac cttgccggga
     4981 ggtactgcaa tccctgaaat gattgacatt gatcacattt ctgcatttaa actcctgaca
     5041 ttcttatttc acccaatgaa gttatttatt tttaaatgag accaggtcct cattttaata
     5101 gtccctggct ggagaatatt gcacaatggc caacatcgaa atcagacaag aaacgccaac
     5161 tgcgttttat ataaaagttc acgacacaga taatgtggca attattgtta atgataatgg
     5221 cctgaaagca ggaacgcgtt ttccggatgg gctggaatta attgaacata ttccccaggg
     5281 gcataaagtc gcattgctgg acattccggc taatggtgaa attattcgtt atggcgaggt
     5341 gattggttac gccgtgcgcg caatcccacg cggaagctgg atcgacgaat caatggttgt
     5401 actaccggaa gcgccgccgt tacatacgct gccactggca accaaagtcc cggaaccctt
     5461 accgccgctg gaaggataca cctttgaggg ctatcgcaat gccgatggca gcgtgggcac
     5521 caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg tggactacgt
     5581 agtaaaaatc attgaacgcg atctgctacc gaaatacccg aacgtcgatg gcgtggtggg
     5641 gctgaatcat ttgtacggtt gtggcgtggc gattaacgca cccgcggcag ttgtacctat
     5701 tcgtaccatt cacaatattt cgctgaaccc taactttggc ggcgaagtaa tggtgattgg
     5761 tctgggttgt gaaaagttgc agcctgaacg cctactgact ggaacggatg atgtgcaagc
     5821 tattccagta gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg tcggttttca
     5881 gtccatggtc gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg
     5941 tcagcgagaa acctgtccgg cttcagaact ggttgtcggc atgcagtgcg gtggcagcga
     6001 tgcgttttct ggcgtaacgg caaacccggc ggttgggtat gcgtctgatc tattggtgcg
     6061 ctgcggcgca acggtgatgt tttcagaagt cacagaagtg cgtgacgcga tccatctgct
     6121 gacaccacgc gcagtgaacg aagaggtcgg caaacggctg ctggaggaga tggagtggta
     6181 cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa
     6241 gaaaggcggt ctggcaaacg tggtagagaa ggcactcggc tccattgcta aatcgggtaa
     6301 aagtgcaatt gttgaagtgc tgtcgcccgg ccaacgcccg actaaacgcg gattaattta
     6361 cgccgcgacg ccagccagcg attttgtctg tggcacgcaa caggtggctt cgggtatcac
     6421 cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc cttatggcgg tacccgtcat
     6481 taaaatggcg acccgcaccg agctggcgaa ccgctggttt gatttaatgg atattaacgc
     6541 gggcactatc gctaccggcg aagaaaccat tgaagaagtg ggctggaagt tgttccactt
     6601 tattctcgac gtcgccagcg ggaagaagaa aactttctcg gatcaatggg gactgcataa
     6661 ccagctggcg gtgtttaacc cggcaccggt gacctgattt cttttggaac gtccctcgca
     6721 aaacatggcc ttagtgccat gtttttattg tttaaagtcc acacatccat taataatgca
     6781 tttgcattac cttagttcaa gcttataatt tgagcagaaa acaggctgta aaaggacagt
     6841 aaataatgcc cgctaatgct cgctctaacg ctgtactgac cactgaatca aaggtcacta
     6901 tacgcggaca aacaactatc cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc
     6961 tggacagcat tcattacgaa attctgcctg gtgggcaagt atttatgtgc cgactgggag
     7021 atgaacagga ggatcatact atgaatgcat ttttgcgttt tctggatgca gatatccaga
     7081 acaacccgca aaaaactcgt ccattcgaca ttcaacaagg aaagaaactt gtcgctggca
     7141 tggacgtcaa cattgacgat gagattggcg acgacgaata atggattttc cacaaagggt
     7201 taatggttgg gcgctatatg ctcatccctg ttttcaggaa acctacgatg ctttagttgc
     7261 cgaagtcgag gcattaaagg gaaaagatcc tgaaaattat cagagaaaag ctgccacaaa
     7321 gttattggcg gtggtccata aagtgattga ggagcatatc acggtcaatc catcatcacc
     7381 ggcattccgt catggcaagt cgttaggctc tgggaaaaat aaagactggt cacgggtaaa
     7441 atttggtgct ggtcgctatc gtctcttctt tcgttacagt gaaaaagaga aagtcatcat
     7501 tctgggatgg atgaacgatg aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta
     7561 taccgtattc agtaaaatgt taaaaagagg acatcctcct gccgactggg aaagcctcac
     7621 ccaagagaca gaagaaaacc attgatggtg ttcacaatgc ccgccgaacg cattggtgtt
     7681 gaccagcggg catcctttct cattccccga ccagaatcac ttcaacccca gcctttcgca
     7741 gtccttccag actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag
     7801 tatcaatgat cttatgtaaa ctggaacggt tgaacttact ggaatcggtg accacgatga
     7861 tccgttccgc aacttcgcac atccgacggt ttaaacgggc ttcatcttca ttatgcgtgc
     7921 tgacgccgcg ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat
     7981 tttgcagcga ttgctcagcc tgatcaccgt aaaaagattg cgactgacgg cgcaaatgcc
     8041 ccccggtcat cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac
     8101 cgttggtcat cgcaattacg tcggtgtgct tgcgcatcag acgagcaatc tcaaaagtgg
     8161 tggtcccgga gtcgaggatc acccgatgac caggctgaat caactcaacg gcagctttcg
     8221 caacgctgcg tttcatcgcg gtgttcagcg cgcttttatc ttccactgat ggctcgactg
     8281 acggcgttgt gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct
     8341 tttccagaaa cgccagatcg ttgcggatcg tcacagtaga tacaccatac aacgccgata
     8401 gatcattaac ttgcacactt ccttgctggc gcaggcgttg aatgatctgc tctcgtcgct
     8461 cactggtgcc tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt
     8521 tcgtaaaact ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca
     8581 agcccacctt acccattggc gcaagctact ctcgtttcag tggctttcat tatgtttctt
     8641 ttgtgaatca gatcagaaaa ctattatctt tcgttttatt tttatctcac catgacgcag
     8701 tatcaactga aacaaaacga aagattaatg tcgcagtaat ctgaactgga gaggaaagtg
     8761 aaacatctga cagaaatggt gagacagcac aaagcgggca aaacaaatgg gatttatgcc
     8821 gtttgttccg cacatccgct ggtgctggaa gctgcaatcc gctacgccag tgcaaaccaa
     8881 acgccgttac tgattgaagc aacctccaat caggtggacc agttcggcgg ttataccgga
     8941 atgacgcccg ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg
     9001 caggatgcgc tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgcct
     9061 gccgctcagg caatggccaa tgccgatgat ttgattaaaa gctacgtggc ggcaggattc
     9121 aaaaaaattc accttgattg cagcatgtcc tgtcaggacg atccgattcc cttaactgat
     9181 gacatcgtgg ttgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgtgaa
     9241 cactttggcg aagccgatct ggagtatgtc attggtaccg aagtgccggt acctggcggc
     9301 gcgcatgaaa ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg
     9361 gaagcccatc gtcacgcttt tgaaaagcag ggtctgaatg ccatctggcc acgcatcatt
     9421 gccctggtgg ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc
     9481 gccaaagcga ccgccttaag ccagatggtc gaaaactacg aaacgctgat tttcgaagcg
     9541 cactctaccg attatcaaac accgcaatcg ctgcgccagc tggtgattga ccactttgcc
     9601 attctgaaag ttggcccggc gctgaccttt gctctgcgtg aagctctgtt ctctctggcg
     9661 gcgattgaag aagaactggt gccagcaaaa gcctgttctg gtctgcgtca ggtgctggaa
     9721 aacgtgatgc tcgaccgccc ggaatactgg caaagccact accacggtga cggcaacgcg
     9781 cgtcgtctgg cgcgtggtta tagctactcg gatcgcgtgc gctattactg gccggacagc
     9841 cagattgatg acgctttcgc tcatctggtt cgtaatctgg cggattcacc aattccgctg
     9901 ccgctgatca gtcagtatct gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag
     9961 ccaacgccac gggaactcat tatcaaccat attcaggaca tcctggcgca gtaccacaca
    10021 gcctgtgaag gccaataagc aaaacaaaga ggaacacgct atgccaaata ttgttttaag
    10081 tcggattgat gaacgcttga ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg
    10141 ggcaaatctg gtgctggtag ccaacgatga ggttgccgaa gatctggtac aacaaaacct
    10201 gatggaaatg gtactggcag aagggatcgc cgtacgtttc tggacgctgc aaaaagtcat
    10261 cgacaacatt catcgcgccg ccgatcgaca gaaaatcctg ctggtttgta aaacaccggc
    10321 cgatttcctg acgctggtga aaggtggcgt tccggtgaat cgcattaacg ttggcaatat
    10381 gcactacgcc aatggcaagc aacaaatcgc caaaacggtt tctgtggatg cgggtgatat
    10441 cgcagcattt aacgacctga aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac
    10501 agagcctgct gtagacctct ttaaattact ttgagggatt catcatggaa atcagtctgt
    10561 tgcaggcatt tgcgttgggc attatcgcct ttatcgctgg cctggatatg tttaacggct
    10621 taacccatat gcaccgcccg gtggttctcg gcccgttggt cgggctggta cttggcgatc
    10681 tgcataccgg tattttaacc ggcggtacgc tggaactggt gtggatgggg ctggcccctc
    10741 tggcgggcgc acagccgcct aacgtgatta tcggtactat cgtcggcacg gcgtttgcca
    10801 ttactactgg cgtgaaaccc gatgtcgcgg tcggtgtcgc cgtacctttc gctgtcgcag
    10861 tacagatggg gattaccttc ctgttctcgg tgatgtccgg cgtgatgtcc cgatgcgacc
    10921 ggatggcgga gaacgccgat actcgcggca ttgaacgcgt gaactatctg gcactgctgg
    10981 cactcggcac tttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg
    11041 caaaaaccat cattgatgtc ctgccgcaac gattaattga tggcctcggc gtcgcgggcg
    11101 gcatcatgcc agcaatcggt tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca
    11161 tcccctactt catcctgggc tttgttgccg cggcctggct caagttaccg gtgctggcta
    11221 ttgctgccgc tgcgctggcg atggcgctga tcgacctgct gcgtaaatct cctgaaccga
    11281 ctcaacctgc ggcacagaaa gaggaatttg aagatggcat ctaatcaaac caccctgccg
    11341 aacgtcagcg aaaatgaaga aacactgctg actggcgtca atgaaaacgt gtatgaagat
    11401 cagagcattg gcgcggagct gacgaaaaaa gatatcaacc gcgtcgcctg gcgttccatg
    11461 ctgttacagg cttctttcaa ctacgaacgt atgcaggctt ccggctggct gtacggcctg
    11521 ctgccagcac tgaaaaagat ccacactaat aaacgcgacc tggcgcgcgc catgaagggg
    11581 catatgggtt tcttcaatac ccatccgttt ctggtgacgt ttgttatcgg cattatcctt
    11641 gcgatggagc gttctaagca ggacgttaac agtattcaaa gcaccaaaat tgccgtcggt
    11701 gcgccgctcg gcgggattgg cgatgcgatg ttctggctaa cgctactgcc gatttgtggc
    11761 gggattggtg caagcctcgc gctacaaggc tctattcttg gcgctgtcgt ctttattgtg
    11821 ctgttcaacg tggtacacct ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg
    11881 ggcgtggcgg ctattccact cattaaagca aataccaaaa aagtcggcca tgcggcatct
    11941 atcgttggga tgacggtaat cggcgcgctg gtggcaacct atgttcgttt aagcaccacg
    12001 ctggaaatca cagcgggcga cgcagtggtt aagttacagg ctgatgttat cgacaaactg
    12061 atgccagcct tcttaccgct ggtctacacc ctgaccatgt tctgtctggt acgccgcggc
    12121 tggagtccgc tgcgcctgat tgcagtgacc gtggttctcg gcatcgtcgg taaattctgc
    12181 catttccttt aaatacaatg aggtattaga tgttaagtat tattttgaca gggcatggcg
    12241 gatttgccag cggtatggaa aaagcaatga agcaaattct cggtgagcaa tcgcagttta
    12301 tcgccatcga ttttccggaa acctcaagca ccgcgcagct tacatcgcag cttgaagagg
    12361 ccatcgccca actggattgt gaagacggca ttgtttttct gacggatttg ctgggcggca
    12421 cgccgtttcg cgtagcttca acgctggcga tgcaaaaacc gggctgtgaa gtgatcaccg
    12481 gcacaaattt gcaactatta ctggaaatgg tgctggaacg cgaagggtta agcggtgaag
    12541 agttccgtgt gcaggcgctg gagtgtggac accgtgggct aaccagtctg gtggacgaac
    12601 ttggtcgctg ccatgaggaa tgtccggtcg aggaaggaat atgacacacg ttctgcgcgc
    12661 cagaaggctg ctgactgaag agggatggct cgatgaccat cagttgcgta ttgctgacgg
    12721 tgtaatcgca gcaatcgaac cgattccagt gggcgtgact gaacgcgatg tggaactgct
    12781 ctgccccgct tacatcgaca cccatgtaca cggtggtgcg ggcgttgatg taatggatga
    12841 cgcgccggat gcgctcgaca agctggcaat gcacaaggca cgcgaaggtg tcggcagttg
    12901 gttaccgacc accgtaaccg cgccgcttaa taccattcat gcggcgctga aacgtattgc
    12961 tctacgctgc cagcgtggcg gacctggtgc gcaagtgctg gggagttatc tcgaaggacc
    13021 gtacttcacg ccgcagaata aaggcgcgca tccaccggag ctgtttcgcg agcttgaaat
    13081 tgccgagctg gatcaattga ttgccgtttc tcagcatacc ttgcgcgtgg tagcgctggc
    13141 accagaaaaa gaaggggcat tgcaggccat ccgccatctt aaacagcaaa acgtacgggt
    13201 gatgctgggg catagcgcgg cgacctggca acaaacccgt gccgcgtttg atgctggtgc
    13261 cgacggcctg gtgcattgct ataacgggat gacaggttta catcaccgcg aaccgggaat
    13321 ggttggcgcg ggattaacag acaagcgcgc ctggctggaa ctgatagccg atggtcatca
    13381 tgtacatccg gcggcaatgt cgctctgttg ttgctgtgcg aaagagagga tcgtactgat
    13441 caccgacgcg atgcaggcag ccgggatgcc ggatggtcac tatacgttat gtggtgaaga
    13501 agtgcagatg cgcggtggcg ttgtccgtac cgcgtccggt gggctggcgg gcagtacgct
    13561 gtctgtcgat gcggcagtgc gcaacatggt cgagttgacg ggcgtaacgc ctgcggaagc
    13621 catccatatg gcgtcgctgc atccggcgcg aatgctgggt gttgatggtg ttctgggatc
    13681 gcttaaaccg ggcaaacgcg ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca
    13741 aatctggatt cagggtcaat tagcttcgtt ttgatggttt gctcctttat taggccttca
    13801 cttcccccgt aaggcctttc tttttctttc gttttgatct gtgcagcggt gtcggatgcg
    13861 acgctaatgc gtcttatccg acctacggtt ggtgagcgta aggccggata aagtatttac
    13921 gccgcatcca gcaatcccgt ttacttccct taccttttct ttcaacgatc acagatttcg
    13981 ttttatttcc ttttcctcca ttgaactttc agtttctttt ctatagattt taaccaacga
    14041 aagaaatcac caagtgaaat gaaacgaaag gcaagcgaaa gcgacaacgc cgacgtcaag
    14101 ttcatcagac taaggattga gttatgccag aaaattacac ccctgctgct gccgcaaccg
    14161 gtacatggac tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca
    14221 acattgacgc gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga
    14281 atctgcaaat catcctgacc ggagccggaa catcggcatt tatcggtgac atcatcgcgc
    14341 cgtggctcgc cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca
    14401 ccaatccaat ggactacctg aacccagctc atccgctact attgatctcc ttcggtcgat
    14461 ccggcaacag cccggaaagc gtcgccgccg tgaaactggc aaatcaattt gtaccagaat
    14521 gctatcacct gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca
    14581 gcgacaacgc gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc ttcgcgatga
    14641 ccagcagcat taccaccatg atggccagct gcctcgcggt tttcgcacct gagatgatca
    14701 acagccaaac cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg
    14761 atttcagcga aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg
    14821 gtggcttaca gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg acagcgggta
    14881 aactggcggc cttctatgat tccccgaccg gattccgtca tggcccgaaa tcgctggtcg
    14941 ataacgaaac gctggtggtg gtgtttgtct ccagccaccc ttacacccgt cagtatgatc
    15001 ttgatctgct ggcagaactc cgccgcgata accaggcaat gcgcgtaatc gccatcgccg
    15061 cggaaagcaa cgacgttatt gccgccggtc cacatatcat cctgccgccg tcccgtcact
    15121 ttatcgacgt tgagcaggca ttttgcttca tgatgtacgc ccagacattt gcactgatgc
    15181 agtcgctgca catgggtaat acgccggata ccccatcagc cagtggcacc gttaaccgcg
    15241 tggtgcaagg cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta
    15301 tctccaccaa atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgccttta
    15361 acattcataa cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc
    15421 cggtgatcct cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg
    15481 ccctgtgtag tgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc
    15541 acgaatcgct ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagc gcgatgatcg
    15601 acggcagtca tttcccgttt gccgagaacg tgaagctggt gaaatcggtt gtcgacttct
    15661 gccactccca ggattgcagc gtggaagcag aactgggccg cctcggcggt gttgaagatg
    15721 acatgagcgt tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg
    15781 tcgaactgac tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca
    15841 gcaaaacgcc gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc
    15901 ctctggtgct gcatggtgcc agcgatgtcc cggatgaatt tgtccgtcgc actattgaac
    15961 ttggcgtaac aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta
    16021 aagcctggtt tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa
    16081 tggatgcgat gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa
    16141 tttcagcata atcattagtt ttttatgatt tatccc
//
LOCUS       DAZKHK010000114        15829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKHK010000114 DAZKHK010000000
VERSION     DAZKHK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.114"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            112..669
                     /locus_tag="ACHOS5_003828"
     CDS             112..669
                     /locus_tag="ACHOS5_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9778574.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAIEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPVEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKAANRFAVPPPPRLVVNNDNEGNG"
     gene            666..2327
                     /locus_tag="ACHOS5_003829"
     CDS             666..2327
                     /locus_tag="ACHOS5_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9778575.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVASGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            2391..4328
                     /locus_tag="ACHOS5_003830"
     CDS             2391..4328
                     /locus_tag="ACHOS5_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9778576.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGVHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            4373..4594
                     /locus_tag="ACHOS5_003831"
     CDS             4373..4594
                     /locus_tag="ACHOS5_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778577.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            4540..7124
                     /locus_tag="ACHOS5_003832"
                     /pseudo
     CDS             4540..7124
                     /locus_tag="ACHOS5_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            7121..7447
                     /locus_tag="ACHOS5_003833"
     CDS             7121..7447
                     /locus_tag="ACHOS5_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9778578.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            7457..7807
                     /locus_tag="ACHOS5_003834"
     CDS             7457..7807
                     /locus_tag="ACHOS5_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9778579.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            7804..8250
                     /locus_tag="ACHOS5_003835"
     CDS             7804..8250
                     /locus_tag="ACHOS5_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9778580.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VASRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            8247..8591
                     /locus_tag="ACHOS5_003836"
     CDS             8247..8591
                     /locus_tag="ACHOS5_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP9778581.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            8657..9373
                     /locus_tag="ACHOS5_003837"
     CDS             8657..9373
                     /locus_tag="ACHOS5_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9778582.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            9388..9762
                     /locus_tag="ACHOS5_003838"
     CDS             9388..9762
                     /locus_tag="ACHOS5_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9778583.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLHQALELIADAESARKK"
     gene            9858..10067
                     /locus_tag="ACHOS5_003839"
     CDS             9858..10067
                     /locus_tag="ACHOS5_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP9778584.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEADPLAALEDALLFASEN"
     gene            10131..10544
                     /locus_tag="ACHOS5_003840"
     CDS             10131..10544
                     /locus_tag="ACHOS5_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778585.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLEKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
     gene            10608..13850
                     /locus_tag="ACHOS5_003841"
     CDS             10608..13850
                     /locus_tag="ACHOS5_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9778586.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAITQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEERTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            13843..14184
                     /locus_tag="ACHOS5_003842"
     CDS             13843..14184
                     /locus_tag="ACHOS5_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9778587.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            14383..15537
                     /gene="ompC"
                     /locus_tag="ACHOS5_003843"
     CDS             14383..15537
                     /gene="ompC"
                     /locus_tag="ACHOS5_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9778588.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQ
                     VQGLNGTADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSET
                     QNMTTFGDQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVK
                     YIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            15747..>15829
                     /locus_tag="ACHOS5_003844"
     CDS             15747..>15829
                     /locus_tag="ACHOS5_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9778589.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDL"
BASE COUNT         3651 a         3687 c         5024 g         3467 t
ORIGIN      
        1 ataccttttt ttcgcaaaat gtttacaggg cagcggtaaa cattttccct gccgcctcca
       61 ttcttaccgg gaggattgct gacgtttttc tgtttttccc cggaggaaag aatgctgacg
      121 acgcaaaaaa gaaaatttgc gctggcgctc atgtcgggca aaaacagaac ggctgctgct
      181 attgaggctg gttactcgga aaagtccgcc agatgtaaag gctcacaact ggcaaaagat
      241 ccggaagtgc tcgcgtttat agcccgtaag cagggcgaga cggtggaggt ggatgaggtt
      301 cctgtttacc ggcagaaaaa gacgcaggcg tgccaccggg aagttcctcc ggtggaaaag
      361 aaaaaccagg tttcagtgcc accggatgac gggcaaagtt tctctccact atccggtatt
      421 gattacatgg aggacggcct tcctgatcct gtgaaagcca tgggtcggat cctggtggaa
      481 aacatcagca ttgatccgaa actggctctg gatgcagcct ggcgactggc gcagttcacg
      541 caccacaaaa aaggggatgc cggtaaaaaa tcggcaaaag gtgacgcggc gaaaaaagcg
      601 gctaaccgtt ttgcggtgcc accaccaccc cgcctggtgg tgaataatga taatgagggc
      661 aacggatgat acctgtgtgg agcacggcct gcccggactg ggcagagcgt ctgaaaaagg
      721 ggctgtcgat tattccggct ccgatttacc cggaccaggc cgcacatgcc ctggcgattt
      781 ttaaacaact gcggattgtg gatgcaccgg gcagcccgac gttcggggag tcctgtgcac
      841 cgtgggtgtt tgatctggtg gctgccctgt ttggctccta cgatgcgcag accggtgtac
      901 gccatataaa ggaagtgttc attctgatac ccaaaaaaaa cagcaagtcc acgctggctg
      961 ccgggatcat gatgacggcg ctgttactga actggcggca ggcggcgggc tacaccattc
     1021 tggccccgac cgtggaggtg gcggctaacg ccttcaaccc tgccagggat atggtacgac
     1081 gggacgatga tctggatgac ctctgtcagg tgcagacaca tatccggacc atcacccatc
     1141 gggtgacgga caccaccctt aaggtggtgg ctgccgatcc gaatacggtg tccggtatca
     1201 agtccgtggg gacgctgatt gatgaactgt ggttatttgg caagcagtac aaagcggagg
     1261 acatgttacg tgaagccatc ggcggccttg cctcccgccc ggaagggttt gtggtgtaca
     1321 caaccaccca gtcgaatgag ccgcccgccg gggtgttcag acagaaactg cagtacgccc
     1381 gggatgtgcg cgacggcaaa attaatgatc cgcactttct gccggtgata tttgagcatc
     1441 cgccggagat ggtggagcgc ggtgagcacc tgctgatgga aaacctcgcc atggttaacc
     1501 cgaatctcgg ttattcggtg gatgaggctt ttctgtaccg ggagtaccgt aaagcccggg
     1561 aggctggtga ggaggcattt cgtggcttca tgtcaaaaca cgccaatgtg gaaattggtc
     1621 ttgccctgcg ttctgaccgc tgggcgggtg cggatttctg ggagcagcag ggcaggcgcg
     1681 tcagcctgga cgatatcctg cagcgcgctg atgtggtgac ggtggggatt gacggcgggg
     1741 gcctggatga tctgctggga atgtacgtga ttggtcgtga cagggaaacc cgcgaatggc
     1801 tgggctgggg ccatgcctgg gcgcatgaaa ccgcggtggt cagacggaag agtgaggcat
     1861 cccggtttca ggattttgtg gccagtggag acatgacgat tgtccgtcgg gtcggggatg
     1921 acacggcgga agtggcggag tatgtgcgtc gtattcatga ggctgagtta ctggaccata
     1981 tcggtattga cccgtcaggt gtggggcaga ttctggattc actggcagaa gccgggatcc
     2041 ccgacggaat tgttgtgggg ataagccagg gctggaagct gggcggggcc atcaaaacca
     2101 ccgagcgcaa actggctgag ggagtgctgg tgcatggtgg tcagccactg atggcctggt
     2161 gcgttggcaa tgcccgggtg gagcctaaag gtaacgccat ccttattacc aaacaggcca
     2221 gcggacgggg gaaaattgac ccgctgatgg cgctgttcaa tgcggtatcc ctgatgtccc
     2281 tgaatccgga gcccaaaaag aaagaatatg cggttttttt catataaccc tgttcaccct
     2341 gtaaccatca tggaccgctg cggcggtttt tttattttca ggaggctgat gtgactctta
     2401 aacgggcctg ctccctgctg acggtgaaat ccttcagtga ggatgagcgg gtgatcaccg
     2461 ggattgcgtc aacgccttct ccggatcggg atggtgacat cctggagccg gagggcgcgg
     2521 agtttggcag tgcgatcccg tttctctggc agcatgacca ttcccgcccg gtggggcagt
     2581 gtacggtgcg ccgggtcagc gaagggctgg aaatcacggc aacactggtg aaacccgtac
     2641 cggatatgcc gtcgcaactg gctgcccggc tggatgaggt ctgggcggcc attaagaccg
     2701 ggctggtcag ggggctgtcc gtgggcttcc gtcctcatga atacaccttt ctggacggag
     2761 gcggagtgca ttttctgcgc tgggagctga tggaggtgtc tgccgtcacc gtgcccgcga
     2821 atgcggaatg cactatccgg accattaaat cttacgaccg cccgttttct gccgcgtccg
     2881 gcaaccggaa accggtggtg aaaatcgcat cttctgccgg cgctgcggca cagtcaacaa
     2941 ccgtttttca taaggaaaag accataatga atattggcga acagattaaa agttttgaaa
     3001 acaagcgtgc agcgctggca gcctcccttg aggaggtcat gaccaaagcc gcagaggaag
     3061 ggcgcacgct ggatgtggag gaggaagagc attacgacaa caccgcagcg gaaatccgtc
     3121 aggtggatgc gcacctgaag cgcctgcgtg aactggaagc cggtaaggcc gccacggcgc
     3181 agccggtgaa acaggccggt aacgggaatg tggccgcggt ggcttctgcg ccggtgatcc
     3241 gtgtggagca gaaactggat aaggggattg gtttcgcacg ttttgccaaa tcgctggctg
     3301 cggctaaagg tgtccgctct gaagccctgg aagtggcccg tcgtcagtat ccggatgaca
     3361 gtcgtctgca tcatgtcctg aaatcggcag tgggcgcggg gaccaccacg gatccgcagt
     3421 gggcaggcag cctgtctgaa tatcaggaat acgcacagga ctttattgat tacctgcgtc
     3481 ctcagaccat tatcgggcga tttggtcagg gcgggatccc tgcacttcgt caggtgccgt
     3541 tcaatatccg tgtgcacgcc caggtgtccg gcggtgctgc cggctgggtg ggtgagggta
     3601 aggcaaaacc cctgacgaag tttgattttg aatccatcac cttcagtcat gcgaaggtgt
     3661 cggccattgc ggtactgacg gaagaattga tccgtttttc cagtccggct gctgatgcac
     3721 tggtccgtaa tgcgctggcg gaagcggtgg tggcgcgtct ggatacagac tttgtggacc
     3781 cgaaaaaagc ggcggtggca gatgtctccc cggcgtccat cacccatgat gtgaagggca
     3841 cggcatcaag cggtaacccg gatgcggatg cagaggctgc gtttggacag tttgtggcag
     3901 caaacctgca acccaccggt gcggtctggc tgatgtccag caccaatgcc ctggcactgt
     3961 ccatgcgtaa aaatgcgctg ggtcagaaag aatacccgga catgaccctg ctgggtggct
     4021 ccttccaggg gctgccggtg attgtctccc agtacgtggg tgaccagctg gtgctggtga
     4081 atgccccgga tatttatctg gcggatgacg gcggcgtggc agtggatatg tcccgcgagg
     4141 catcactgga aatgcagtct gagccgaccg gcgacagtac cacgccgtcg ccggtggagc
     4201 tggtttccat gttccagaca ggcagtgtgg ccatccgtgc ggagcgctgg atcaactggc
     4261 gtcgtcgccg taccgcggcg gtggcggtga tcaccggtgt gaactacggc agtgcgtccg
     4321 gcggctgagt ctgataagga ggacgggagg cgtgcgcctc ccgtaacagg ttatggcaaa
     4381 gatccgatac ctgcagggca cgcatgatgc ccgggccggg gatatccggg atgtggcaca
     4441 gccgtgtgcg gaggtgctgg ttcgcctggg aaaggcagag tacatcacgg tgcgacgtcc
     4501 ggcaggtcag aaaaagaaac gtgatgcgga gcatggcgaa tgtggaacct tttacggcga
     4561 acccgaaaaa accagaaatc aggacgtgac gtaagagagg cgggctggac cagcctgttt
     4621 caggcggtgg ctgagccctt ttccggcgcc tggcagcagg gcgtgaaagc cgatcctgaa
     4681 gccgtcctct cctttcatgc ggtgtttgca tgtatttcgc tgatatccca ggatatcgcc
     4741 aaaatgcggc tgcgtcttat gcagacggat gcgcagggga tacgcaggga aacgcgccgg
     4801 ggagatattg cccgcctctg tcgtcgtccc aacgcccagc agaaccgcat ccagtttttt
     4861 gaactgtggc tgaacgccaa actgcgtcac ggcaatacgg tggtgctgaa aatccgtgat
     4921 gcccgggggc agatcaaaga actgcgtatt ctggactgga accgggttga acctctggtg
     4981 gcggatgacg gcgaggtgtt ctaccgcatc acgccggacc ggaactgtgg gatcactgag
     5041 gcggtgacgg tgcctgcccg ggaagtgatc cacgaccggt ttaactgttt ttttcatccg
     5101 cttatagggt tgccgccggt gtatgccgct gggctggcgg ccacgcaggg gcatcatatt
     5161 caggaaaatt cgacgtattt tttcagaaat ggcggcaggc catccggggt gattgagatc
     5221 cccggcagta ttacggaaga aaatgcgaaa aaactgaaga gcaactggga cagcgggtat
     5281 acaggcgaaa atgcggggaa aacggccatt ctgagcaacg gggcaaaata caaccccacg
     5341 acgttttcac cggtggatgc gcagacggtg gaacaactga agatgaccgc tgaaattgtc
     5401 tgttcggtgt tccgtgtccc ggcctacaag attggcgtgg gacaaccgcc ttccagtgac
     5461 aacgtggagg cgctggagca gcagtattat tcccagtgcc tgcagacact gattgagtcc
     5521 attgagctgt tactggatga ggcgctggaa acgggggaaa acgagagtac ggaatttgat
     5581 gtcaccacgc tgctgagaat ggacagtgag cggcgcatga aaacgctggg ggatgcggtg
     5641 aaaaatacgc ttctcacgcc caatgaagcc cgtaaacggg agaacctgcc gcccctggca
     5701 ggaggcgatg cactgtatct tcagcagcag aactacagtc tggaagcgtt gtcccgccgt
     5761 gatgcccgtg aagatccgtt cgcgtcgtcc ggtaaaacag cttccgtgcc tcaggcggtc
     5821 gctgcatctg acggtaataa ggcaatcact gaaacagagc atgacgcggt gaaggcgatg
     5881 ttcaggggga ttctgaaaaa atgaatgaac gtgaactgtc cattatccgt gcgctgggtg
     5941 aagaattttc cgcggtgctg gcggatttac agcgcacatt tgaggagaaa atagccgcgc
     6001 aggcacaaac gtttgaagaa aaactggctt ccctgtctgt ggtattacag aagtgcgtga
     6061 cgggcgatga tgtgcgtccg atgcttgagc agatggtgaa ggaggcggta agccatatcc
     6121 ctgttccgcg agatggtcgt gactacgatc cggaagtact gcagaaggcg gtgaatgatg
     6181 cggtcgcaaa tattccgcag ccggcggacg gtaaaagtat caccccggat gatgtgcgtc
     6241 cgatgcttga acagatggtg aaagaggcag tgagccatat tcctgttccg cgtgatggtc
     6301 gtgactacga tccggaagta ctgcagaagg cggtgaatga tgcggtcgca aatattccgc
     6361 agctggcaga cggtaaaagc ctcaccccgg atgatgtgcg tccgatgctt gagcagatgg
     6421 tgaaggaggc ggtaagccat attcctgttc cgcgtgatgg tcgtgactat gatccggaag
     6481 tactgcagaa ggcggtgaat gatgcggtcg caaatattcc gcagccggca gacggtaaaa
     6541 gtatcacccc ggatgatgtg cgtccgatgc ttgagcagat ggtgaaggag gcggtaagcc
     6601 atattcctgt tccgcgtgat ggtcgtgact atgatccgga tgttctgcag aaggcggtga
     6661 atgatgcggt gagtgccctg ccggctccgc aggacgggcg tgatgccacg gcactggaaa
     6721 tactccctgc cattgacgat caaaaatcct ttccccgggg cacgtatgcc acacaccagg
     6781 gcggactctg gcgggcgtat gaaaaaacgc acgggatgcg gggatgggaa tgcctggttg
     6841 acggggtggc ggatattgac gtcagcatga cgggtgaacg gttgttctct gtggtggtcc
     6901 agcagagcag tggccagcgt acggaaaaaa cattttccct gccggtgatg ctctaccgcg
     6961 gtgtgttcag agccggtgaa acctaccacc ccggcgatac ggtgacgtgg gggggctcgc
     7021 tgtggcactg caacagtatg accggtgata aacccggaga agctcattca tcaggctgga
     7081 ccctggctgc aaaacgtggg cgggatgctg gaggcggaaa atgacggcat tactgacact
     7141 ggaagagatc aaggcacatc tgcgtgtcga ccatgacgcg gatgatgaca tgctgatgga
     7201 caaggttcgt caggctaccg ccgtgctgct ggcctacatt cagggcagcc gggataaggt
     7261 gattcgtgag gacggtgaac tgatcccggg cgaggcatta acccggatga agggggctgc
     7321 catgcgactg accgggatgc tgtaccggaa tccggatctt gcggagcggg aagaactgct
     7381 tcagggggag ctgccgtttt ctgtttccgt gctgatttac gatttgcgtt gtccgacggt
     7441 gttatgagga gggggaatgg caatatctgc aggtcgtctg acacagatga taagtgttct
     7501 gaacccggtg ttaacccgta atgctgccgg agaaatgacg gaagaatggg tgtcatgcgg
     7561 gaaaattcat gcggatatcc gtggcaggag cagccgggag cggatgcagt ccggtgcgga
     7621 aatggcgcag gcggaaatcc gcatctgggt gcgcggtcag tccggtcggg aaatcacggc
     7681 agcgtcacga cttcatgtgc tgagtggtcc atggcgtgac cggatcctga acgttgtcgg
     7741 gctgcccgtg ccggatgcga ccggcgggcg tctggaaatt ctctgtcggc tgggagggga
     7801 aaaatgatcg aaaccctgct ggatttttcg gggctggagg agatcagccg cgatttgcag
     7861 cttctgagtg gtgcggaaaa taaccgggtg ctgcgtgagg caacccgtgc gggtgcgaat
     7921 gtgctgaaag aagaagtggc gtcacgggca ccggtacgca ggggaaaact gcgccgcaat
     7981 gtggtggtcc tttcccggcg ctcccgcgat ggcgggatgg aatccggtgt ccatatccgt
     8041 ggtgttaatc cggacaccgg taacagcgat aacaccatga aggcggataa cccgcgcaat
     8101 gctttctact ggcggtttgt ggaaatgggg accgtgaata tgccaccgca cccgtttgtg
     8161 cgcccggcgt ttgatgtgcg cagtgaacag gcagcgcagg tggcgattgc tcggatgaac
     8221 cgggccattg atgaggtact gagacgatga cggaggcgga tttgtatcct catctggcgc
     8281 atcttgccgg cgggcaggtg tacccgtatg tggtccccct gctggatggc aggccgtcgg
     8341 tggcgcttcc gtgggtggtt ttcagcctga tttcatcggt gtctgcggac gtgatgggcg
     8401 ggcaggcgga gtcctcagtg gcggtgcaga tagacgttta tgccgggact gtgacgcagg
     8461 cgcgtcagat acgtcaggac gcccgtgaag ccataatgct gctggccccg ggatccgtca
     8521 gtgaaatgca ggactatatt ccggaaaacc gctgttaccg tgcaaccctg gagtttcagg
     8581 tcacggtgtg actttttctt ttttctacaa aacccatacc ccgccgcgtg cgggtttttt
     8641 attatcagga ggcagaatgt ctgctttgta tgaacgctca cagctgacgc aggtgatgat
     8701 ttcatctgcc ccggcgactg ctgaaactat ggataaggcg gaatatctgc gcctggactg
     8761 caccatcaag gaagtccagt tcaccgccgg tcagaaacag gatattgatg tgaccacgct
     8821 ctgctccaca gagcaggaga atatcaatgg tctgggggcg tcgtccgaga tttccatgtc
     8881 gggtaatttt tatctgaatc aggcccagaa cgccctgcgt gatgcttatg acaatgacgc
     8941 gttgtatgcg tttaaggtgc agtttccgtc cggtaagggc tttaaattcc tggcggaagt
     9001 gcgccagcac acctggtcat ccggtaccaa cggcgtggtg gctgcaacgt tctcactgcg
     9061 tctgaaaggc aaaccggtgt cctttgtggt accgctggcg tttgtgaaaa atctggataa
     9121 gacacttacc gtgaataccg gtgcgctgct gacaatgtca gtcagtgcca acgggggaac
     9181 gccgccgtat aaatacgcct ggaagaagga tggtcagccg gttgacgggc agacgacaga
     9241 caccttcagt aagccaggtg cgcagtccgc tgatgcgggg aaatatacct gcgtggtgac
     9301 tgattcggca gagaaagcac agagtgtgac gtctgttgaa tgtacagtga cagtgagcgc
     9361 agccgccgga taaggggatg ggtcatcatg aaaaaggatc tgaaaacgct ggcgctggcc
     9421 agactgtcag ggtttcgtca taaaacggtg aaggtgccgg aatggggtaa tgtcagcgtg
     9481 gtgctgcggg agccttcggc agaggcctgg tatctgtggc aggacgtgct caatggtgat
     9541 ggagaggatg acgataccct gtcggtggtg gcgaaaaccc gccgtaacct ggaagcggat
     9601 gtgacgctgt tctgcgatgt cctgtgtgat acggacctgc agcgggtgtt cactccggac
     9661 gaccgtgagc aggtgctggc cgtctatggt ccggtacatg cccgcttgct gcatcaggca
     9721 ctggaactga tcgctgatgc agagtcggcc agaaaaaagt agcccgcccg gaaattcgct
     9781 ttctgatgcg acttgcgctc cgtctggggc gcaccttatc cgaactgcgc cacagcctga
     9841 gtgcgagcga ggcgatgatg tggatggagt tcgacagggt atccccgctg ggtgatgagc
     9901 gcggggatat ccgtaatgca cagatcgtga aagcggtttt cggggcacag gggatgaatg
     9961 ttgcactgaa ggacgccatg ctctgctggg gcgaggatga ggataagccg gaggcagatc
    10021 ctttggccgc tctggaggat gctctgttat ttgcttcgga aaattaattt taactttgca
    10081 gatctttatt acagtatttt cggggaaaat taactgaacg gagccatttt atgaagaaat
    10141 tcatgctgtt gattttctct gtcagctttt ttctgacggg gtgtgcaacc attgtcggag
    10201 ataaaacgca gactgtacag gtaaacagta atccgtctgg tgcggatttt attattaaag
    10261 atgagtcagg gaaaacaatt tcttcgggca aaacaccgca gaatgtaaca cttgaaaaat
    10321 ctgacggcag ttacttcggg aaaaagagtt atcagatcac attcagtaaa gagaattatc
    10381 agcccgtaac gctgccgatc aaggcatcgg ctaatggatg gtacattggt ggtaactttg
    10441 tctttggtgg tattgttggc tggttgcttg tggacccatt caatggcggt atgtatacac
    10501 tgagtccgga aagtctgaat cccgggctgg tacccaaaac ttaatcaata atctgctgat
    10561 gaataaaacc tgctgcggca ggtttttttt cgcccggaga aaggtgaatg gcgacgttac
    10621 gtgaactgat tatcaaaatt tcggcaaatt cacagtcatt ccagtcggag atccagcggg
    10681 cgtcccgtat gggcagtgaa tattaccgga ccctgcagaa tggcggacgt caggctgctg
    10741 cggcagccag ggagcagcgc cgggctctgg ctgagctgaa cagccagttg acggaaatcc
    10801 gcgcttcggc tgtcggaatg accggtgcgt ttgctggtgc ctttgccacc ggacacctga
    10861 tttcgctggc ggatgaatgg agctccgtga atgcccgtct gaaacaggcg tcgcagtcat
    10921 cggatgaatt ttcgtcatca cagaaagtgc tgatggatat cagccagcgg acaggcaccg
    10981 cattttcgga taatgcggcc ctgtttgccc gttcggctgc ctcgatgcgt gaatatggtt
    11041 acagtgctga tgatgtgctg aaggtgacgg aggccatttc gacagggctg aaaatttccg
    11101 gtgccagtgc ggcagaggcg ggttcggtga tcacccagtt cagccaggcg ctggcacagg
    11161 gggtgttgcg cggcgaggaa tttaattcgg tcaatgaaag tggggaccgg atcattcgcg
    11221 cactggctgc aggcatgggc gtggcccgta aggatctgaa ggcgatggcg gacgatggtc
    11281 aactgacggc ggataaagtc gttcccgcgt taatcagcca gctggggata ttgcgtgatg
    11341 aatatgcagc catgccggaa acggtgtctg acgggatcac gaaggtggaa aacgccttta
    11401 tggcctgggt gggcggcgcg aatgaggcca gcggagcgac gaaaacgctc tccggcgcac
    11461 tgaacggtgt ggccggaaat attgataccg tggcaacagc tgcgggtgtg ctggttgctg
    11521 tcggggtggc ccggtacttt ggtaatctgg cttccggagc gatgtctgcc acggcaggac
    11581 ttgtgaccgc tgcacgtaat gaagtggcac tggctgaggc ccagttaagg ggaacgcaga
    11641 ttgccacggc gcgggcaagg gcagccgtgt accgggctca gcaggctgtg gcggcagccc
    11701 gcgggactga gatgcagatt gcggcagagg cccgtctggc gatcacacag gaacgcctga
    11761 acagaaatat tgctgccaga accgccgccc agaatgcgct gaacagtaca acggcggtgg
    11821 gctcacgtct gatgagcggt gcgctgggac tggttggagg cgtacccgga ctggtgatgc
    11881 tgggagctgc cgcatggtac acgctgtacc agaatcagga acaggccagg gagtctgcgc
    11941 gccagtatgc actgacgatt gatgaaatcg cgcataaaac gccatcaatg tctctgcctg
    12001 aagcctcaga taatgaagag cgaacacggg aggcgctgac agagcagaac cggctgattg
    12061 atgagcaggc cagccgggtg aaatccctgc aggaaaaaat cgccgggtat cagtatgtgc
    12121 tggctaatcc gggctggacg accggtaacg gcttcatgat aaaccatctg acatcggtga
    12181 aaactgtaac ggaagagctt gctcaggcaa cagagcagtt tgctgttgag cagtcccgtc
    12241 tggcacagat gcaggaaaaa gcgcagtcca ttcaggatgt gcttgccgga ctggaagagc
    12301 gccgtgttgt gttaattcgt cagcaggcag cagagcagaa taaagcgtat cagtcactgc
    12361 tggtcatgaa cggtcagcat acggaattca accgcctgct ggggctgggt aatgaactgc
    12421 tgcaacagcg tcagggactg gtgaatgtac cgctgcgatt gccacaggcc actctggatg
    12481 ataaacagca gagcgccctg aataacacag agcgtcaact ggccctgtcc cggctgaaag
    12541 gggaagaaaa agagcgtgcc cggctggggt atgcggcgga tgaccttggt ctggtggggg
    12601 atacgtatca ggaggcgagg cagcgttaca tccgtaattc gatggaagcc tggcgcaata
    12661 atgaggcgaa taaacccaaa tcccgggccg gaaaatctga gacggaaaaa gcggaagaca
    12721 gtttttcccg tctgctgaag cagcagaagg aacaactggc actggcaggg aagaatacag
    12781 aactggcgaa gctgaagtac cagacctcgc agggagagct gaaaaccctg acggagatac
    12841 agaagcagga gttgctgcgc aatgctgccc tgattgacca gaagaaaatc cgggagcagt
    12901 tacgggcccg ggaggaggct ctgaaaaatg ataatgcaga cgaaagggca tcaaatgacg
    12961 ctgagctgct ggggtacggg cagggtgagc gggtccgtga acggatgcgg gagctgcagc
    13021 agattcgtga cggctaccgc cagaaggatg cggacctgca gtctcagtat cagacagggg
    13081 atatcagtga ggatttttac agacaggcgc tggcgcagaa tgcgcagtat ctgagtgaac
    13141 gccttaaaga ccaggaggct ttttatgccg aatcggatgc gcagcgtgct gactggcaga
    13201 aagggctgca ggaagggcta agtaactggg tggacagcgc atcagattac gcttcacagg
    13261 cagcacagct tgcgacagac ggtatctcag ggatggtgaa taacatcacg gagatgctga
    13321 acggaaataa agtggaatgg cgcagctggg ctgcatcaat cctgcaggaa atatcaaaag
    13381 ttcttatgaa tgcggccatt gtcaacggca ttaagatggc cgcaaacagt atgtccggtg
    13441 caggaggatt tttcggcagt ataggcaact ggctgggtgg cgcggtggcc aatgcaaaag
    13501 gcggcgttta tacctcggca aacctgagtg catacagtaa cagtattgtg gacacgccca
    13561 cgtactttgc ctttgcaaaa ggggcagggc tgatggggga agccggacct gaagccatca
    13621 tgcccctgac ccgggcagcg gatggttcac tcggcgtgcg tgcggtgggc agtatgaacg
    13681 gcagtgcggg tctggtgtat tccccggtgt accacattgc cattcagaat gacggcgcta
    13741 acgggcagat agggccggaa gcggcgggca cccttgtgca actgattgac cagcgggtac
    13801 aggcggtgat gttatccatg cgtcgtgacg gaggaatgct gagtggatga gattaagacc
    13861 cttcactggt gtccccggga agggatgcag gtgacggaga aaccgtcggt ggtgacggtg
    13921 aagtttggcg acggttatca gcaacgtcgt ccggcaggac tgaatgcgca actgaagacc
    13981 tttcaggtgg tttttcgggt gacaacggat gctgagcggg aggcactgtc cgcgtttctg
    14041 tcatggcatg gtggttaccg ggcttttttg tggaagcccc cgaaacataa ccggacggtc
    14101 agggtggtgt gccgggagtg gagtattacg gataacgccc ggtacagtga tttcagttgc
    14161 acgatagagc aggtagtcag atgagaatta tcctataaat cactctttat tgtttagttg
    14221 ttaattattt ttaatctgtc ttgtcagaaa gtgcgaaatc gatcttgtat ttacattttg
    14281 ttacataata taaattgaac taagaatttg tattaaaata ttttaatttt tgttcatgac
    14341 atctaaatgc atgaatagtt cagtttaaat aaggattaaa tcatgaaaaa aatgacagtg
    14401 gcactttctg ctgtagcagt ggcagtgatg tttgctgcgg gggcgcaggc agcagaagtt
    14461 tataataaag atggtaataa actggatctt tacgggcgtg caaccgctct gcattacttc
    14521 tcggatgata aaggtaatga cggagaccag acttatgctc gtctcggctt taaaggcgaa
    14581 acgcagatta atgatcaact gaccggattt ggtcagtggg aataccagtt ctctggtaac
    14641 aaaacggaat ctgaaggttc cgcgggaaat aaaacccgtc tggcatttgc aggtctgaga
    14701 tttgcagatg ttggtaccat tgattacgga cgtaactatg gtattgctta cgatgtcgga
    14761 tcatatactg acgtactgcc tgagtttggt ggtgatggct ggacgcagac cgataacttt
    14821 atgacggccc gaacttccgg tgtcctgact taccgtaata cagacttctt cggactggtt
    14881 gatggtctga attttgcggc gcagtatcag ggcaaaaatg agcgtgatga ccttcagaag
    14941 gctaatggcg acgggtatgg tttctcggcc agctatgagt ttgatggttt tggttttgtc
    15001 gctgcgtata ccaagtcaga tcgtaccgat aagcaggttc aggggttgaa cggtactgca
    15061 gatgttcttg atcctgaatc aggtaaaaag gttggagaaa aaacagttga ctcaggtagt
    15121 gtggcaaaag gtaaacatgc tgaattctgg ggaactggtc ttaaatatga tgccaataac
    15181 ctgtatctgg cagcagtgta ttctgaaacc cagaatatga cgacctttgg tgatcaaggt
    15241 gttgcggata aagctcagaa tatcgaagcc gttgttcagt atcagtttga ttttggtctg
    15301 cgcccgtccc ttgcctacct tcagtcccgc ggacaggatg ttatggttgg tggtgtgaac
    15361 catggcgatc aggatctggt taaatatatt gatgtcggtg cgacttatta ctttaacaag
    15421 aatatgtcca cctatgttga ttataaaatt aacctgattg atgaaagtga atttacccgg
    15481 aaagccggtg ttgcgacaga taatatcgtt gctgtgggta tgacttatca gttctgatta
    15541 ttgctgataa gttaataaat acgggccgtc tgcccttaca ggcggcctgt atcaatgaaa
    15601 acacagtttt cattggtcac tgcgatcagc aattgccatc tggcatgtgc tgatttaact
    15661 ttctgttatt acctttattg gttttatttt aaattggact tttattgttc ggggcgcgtc
    15721 tgcgcccctt ttttatgggc ggatatatgc aggatattca cgaagaaagc ctgaacgagt
    15781 cggttaagtc agagcagtca ccgcgggtgg tgctctggga aatcgacct
//
LOCUS       DAZKHK010000115        15496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKHK010000115 DAZKHK010000000
VERSION     DAZKHK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.115"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..330)
                     /locus_tag="ACHOS5_003845"
     CDS             complement(<1..330)
                     /locus_tag="ACHOS5_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFP9778590.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPGGGEYWRLKYRV
                     AGKEKLLALGVYPEVTLADAPAKLEEAKRGISGGIDLMEVKREEKIARETQLNNTFKD
                     IALEWHSN"
     gene            complement(485..560)
                     /locus_tag="ACHOS5_003846"
     tRNA            complement(485..560)
                     /locus_tag="ACHOS5_003846"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(525..527),aa:Thr,seq:cgt)
     gene            complement(675..1928)
                     /gene="proA"
                     /locus_tag="ACHOS5_003847"
     CDS             complement(675..1928)
                     /gene="proA"
                     /locus_tag="ACHOS5_003847"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9778591.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(1940..3043)
                     /gene="proB"
                     /locus_tag="ACHOS5_003848"
     CDS             complement(1940..3043)
                     /gene="proB"
                     /locus_tag="ACHOS5_003848"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9778592.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3331..4386
                     /gene="phoE"
                     /locus_tag="ACHOS5_003849"
     CDS             3331..4386
                     /gene="phoE"
                     /locus_tag="ACHOS5_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9778593.1"
                     /translation="MKKSTLALVVMGIVASVSVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4425..4826)
                     /gene="crl"
                     /locus_tag="ACHOS5_003850"
     CDS             complement(4425..4826)
                     /gene="crl"
                     /locus_tag="ACHOS5_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9778594.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4884..6128)
                     /gene="frsA"
                     /locus_tag="ACHOS5_003851"
     CDS             complement(4884..6128)
                     /gene="frsA"
                     /locus_tag="ACHOS5_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9778595.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLLVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(6220..6678)
                     /gene="gpt"
                     /locus_tag="ACHOS5_003852"
     CDS             complement(6220..6678)
                     /gene="gpt"
                     /locus_tag="ACHOS5_003852"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9778596.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            6939..8396
                     /gene="pepD"
                     /locus_tag="ACHOS5_003853"
     CDS             6939..8396
                     /gene="pepD"
                     /locus_tag="ACHOS5_003853"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9778597.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLTDENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8452..9066)
                     /gene="prfH"
                     /locus_tag="ACHOS5_003854"
     CDS             complement(8452..9066)
                     /gene="prfH"
                     /locus_tag="ACHOS5_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP9778598.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKIDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9063..10202)
                     /locus_tag="ACHOS5_003855"
     CDS             complement(9063..10202)
                     /locus_tag="ACHOS5_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP9778599.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESSAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(10393..10845)
                     /gene="yafP"
                     /locus_tag="ACHOS5_003856"
     CDS             complement(10393..10845)
                     /gene="yafP"
                     /locus_tag="ACHOS5_003856"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9778600.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITCIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELMVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(10842..11897)
                     /gene="dinB"
                     /locus_tag="ACHOS5_003857"
     CDS             complement(10842..11897)
                     /gene="dinB"
                     /locus_tag="ACHOS5_003857"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9778601.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(11968..12753)
                     /gene="lafU"
                     /locus_tag="ACHOS5_003858"
     CDS             complement(11968..12753)
                     /gene="lafU"
                     /locus_tag="ACHOS5_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP9778602.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     LPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            12725..14437
                     /locus_tag="ACHOS5_003859"
                     /pseudo
     CDS             12725..14437
                     /locus_tag="ACHOS5_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            14516..14860
                     /locus_tag="ACHOS5_003860"
     CDS             14516..14860
                     /locus_tag="ACHOS5_003860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778603.1"
                     /translation="MIRRVAAHLAPVHNCRMRRERLIRPTGERGFVGMIRRVAAHLAP
                     VHNRRMRRERLIRPTGECGFVGMIRRVAAHLAPVHNRRMRRERLIRPTGERGFVGMIR
                     RVCVASGIGAQL"
     gene            complement(14855..15352)
                     /gene="rayT"
                     /locus_tag="ACHOS5_003861"
     CDS             complement(14855..15352)
                     /gene="rayT"
                     /locus_tag="ACHOS5_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFP9778604.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNVIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
BASE COUNT         3761 a         4082 c         3895 g         3758 t
ORIGIN      
        1 gttactgtgc cactcaaggg caatatcttt gaaggtgttg tttaactgcg tttcccgggc
       61 aatcttttcc tctcgcttca cttccatcag atcgattccc ccagagatac ctcttttagc
      121 ttcttcaagt tttgcaggag catcagctaa ggtgacctca ggatacacac ctagtgctaa
      181 cagcttctct ttaccagcta cacgatactt gagacgccaa tattctcctc caccaggttt
      241 taccaggagg tacagaccac caccatcagc caacttgtaa gccttctctt ttggcttggc
      301 agtgtctatt tgacgggcat tgagtttcac ttgggggtat ctccactaaa ctgaacagca
      361 aatcccctca attgattgta gagtttgggg tacttaaata gacgtcaaaa gactaaaagg
      421 ggcattaata tactgattat aagaggtttt taaatacttg agtagacttg gggagacgtt
      481 agaatggtgc cgataatagg agtcgaacct acgaccttcg cattacgaat gcgctgctct
      541 accaactgag ctatatcggc cctgaaagga catgttcacg aacgtgaatc acggtggaca
      601 aggttaaaac taaccgggcg atgcgtcaat ggccttgtga atcaaatggc tacttttgca
      661 tcacccggtt ttatttacgc acgaatggtg taatcaccaa tgccgatcca cttataagtg
      721 gtcagagctt ccagccccat tgggccgcgc gcgtggagtt tttgtgtgct taccgcaact
      781 tccgccccca gaccaaactg gccgccgtcg gtaaaacgcg tagaggcgtt aacgtaaaca
      841 gcggaggaat ccacttcgtt aacaaaacgc tgggcgttgc gcatatcgcg ggtcaggatc
      901 gcatcggagt gttgcgtgcc gtgttcacga atatgggcaa tggcgtcatc cagatcgcta
      961 acgattttga cgttcaaatc taatgacaga aactcatcgt catactcttc ggctttaaca
     1021 gccaccacct tcgcggggcc tgcctgcaac tgcgccagcg cggctgcatc agcgtgtaac
     1081 gtcacgccgc tttccgccat ttgtttgctt aatgcgggca ggaagctatc tgcgatgttt
     1141 ttatttacca gcaacgtttc taccgtatta catgtgctcg gtcgctgagt tttcgcgttg
     1201 acgatcactt ttaatgcttc agcgatctct gcgctttcat caacgtaaat atggcatacg
     1261 cctataccgc ctgtgatcac cgggattgtc gactgctcgc ggcacagttt atgcaaacca
     1321 gccccaccgc gcgggatcag catgtcgatg tatttatcca tacgcagcat ttcactgacc
     1381 agcgcacggt caggattatc aattgcctgc acggcacccg ccggtaagcc gcaggatttc
     1441 agggcgtcct gaatcaccgc taccgttgca gcgttagtgc gacaggtttc tttgccgccg
     1501 cgcaggatca ccgcgttacc ggttttcagg cacagggaag caacatcaac cgtcacgttc
     1561 gggcgcgctt cataaatcac gccaatcacc cccagcggta cgcgacgacg ctcaagacgc
     1621 aggccgctgt ccagtacgcc gccatcgatt acctgcccca ccggatcggc gagattgcac
     1681 acctggcgca catcgtcggc aatgcctttc agccgtgcgg gcgtcagtgc cagacggtca
     1741 agcatcgctt cgctaaggcc attggcacac gcgtctgcaa catcctgcgc gttagcgttg
     1801 aggatacttt cgctttgtgc ttccagttca tcggcgattt tttccagcac acgatttttt
     1861 tcgcggctgg agagttgcgc taatttatac gaggcttgct tcgcggcaat gcccatttgt
     1921 tccagcatcg gccagctcct taacgggtaa tcatgtcatc acggtgaacg gcaaccgggc
     1981 cgtattcata tcccagtatt gcatcaattt cttgcgagtg gtgtccggca atacggcgta
     2041 atgcatcgct gttgtaacga ctgacgccgt gggcgatatc gcgaccttcg aggttgcaaa
     2101 tacggatgac ttcaccacgc gagaaattgc cagtcacgct tttaatgcct ttcggcaaca
     2161 gggagctgcc gcgttccaga atggcggcag ttgccccttc atctaccgtg atttcacccg
     2221 caggcggcgc accgaaaatc cagcgtttac ggttttcaag cggagtcgcc tgggcatgga
     2281 acagcgtacc gacggaaatg ccttccatca catcaccaat aacgcccggc ttgctgcccg
     2341 cggcaataat ggtgtcgata cccgcacggc aagccacgtc agcggcctgc aatttggtgc
     2401 tcataccgcc agttccgagg cctgaaacgc tatcaccggc aatcgcgcgc agtgcgtcat
     2461 caatgccgta cacatcttta atcagttctg cctgcggatt gctgcgcgga tcagcggtat
     2521 acaaaccttt ttgatcggtc agcagcaaca gtttatcggc acccgccaga atcgccgcca
     2581 gcgcagaaag gttatcgtta tcgccgacct taatctctgc cgtagcgaca gcatcgttct
     2641 cattgattac cggaacgata ttgttatcga gcaacgcacg cagggtatcg cgggcgttca
     2701 ggaagcgttc acgatcttcc atatcagcac gggtcagcaa catctgcccg acatgaatgc
     2761 cgtaaattga aaacagctgt tcccacagtt gaatcagtcg actctgccct accgccgcca
     2821 gcagctgttt tgaagcgata gtcgctggca attccgggta ccccaggtgc tcacgtccgg
     2881 cggcaatcgc acccgacgtc acaataacaa tccgatgccc ggcggcatgt aactgcgcgc
     2941 actggcgaac aagttcaacg atatgggcac ggttcaggcg gcgcgatccg cctgttagca
     3001 cactggtgcc gagttttacc accagcgtct ggctgtcact catgattctc tgccattcaa
     3061 ttttaggaaa aatgatatca aacgaacgtt ttagcaggac tgtcgtcggt tgccaaccat
     3121 ctgcgagcaa agcatggcgt tttgttgcgc gggatcagca agcgtagcgg cagttgttta
     3181 cgcttttatt acagatttaa taaattacca cattttaaga atattattaa tctgtaatat
     3241 atctttaaca atctcaggtt aaaaactttc ctgttttcaa cgggactctc ccgctgaata
     3301 ttcgcgcgtt aattaaaatc aggaatgaaa atgaaaaaga gcactctggc attagtggtg
     3361 atgggcattg tggcatctgt atccgtacag gccgcagaaa tatataacaa agacggtaat
     3421 aaactggatg tctatggcaa agttaaagcc atgcattata tgagtgataa tgacagtaaa
     3481 gatggcgacc agagttatat ccgttttggt tttaaaggcg aaacacaaat taacgatcaa
     3541 ctgactggtt atggtcgttg ggaagctgag tttgccggta ataaagcgga gagtgatact
     3601 gcacagcaaa aaacgcgtct cgcttttgcc gggttgaaat ataaagattt gggttctttc
     3661 gattatggtc gtaacctggg cgcgttgtat gacgtggaag cctggaccga tatgttcccg
     3721 gaatttggtg gcgactcctc ggcgcagacc gacaacttta tgaccaaacg cgccagcggt
     3781 ctggcgacgt atcggaacac cgacttcttc ggcgttatcg atggcctgaa cttaaccctg
     3841 caatatcaag ggaaaaacga aaaccgcgac gttaaaaagc aaaacggcga tggcttcggc
     3901 acgtcattga catatgactt tggtggcagc gatttcgcca tcagtggagc ctataccaac
     3961 tctgatcgca ccaacgagca gaacctgcaa agccgtggca caggcaagcg tgcagaagca
     4021 tgggcaacag gtctgaaata cgatgccaat aatatttatc tggcaacttt ctattctgaa
     4081 acacgtaaaa tgacgccaat ctctggcggc tttgccaata agacgcagaa ctttgaagcg
     4141 gtcgctcaat accagtttga ctttggtctg cgtccatcgc tgggttatgt cttatcgaaa
     4201 gggaaagata ttgagggcat cggtgatgaa gatctggtca attatgtcga tgttggtgct
     4261 acgtattatt tcaacaaaaa tatgtcagcg tttgttgatt ataaaatcaa ccaactggat
     4321 agcgataaca aattgaatat taataatgat gatattgtcg cggttggcat gacctatcag
     4381 ttttaatgga tattgccgga tgtgatgcat ccggcacatt tcattcacgc cgttaacttc
     4441 accggttcat cacgaaaatc atctgccggt tccagcttca gattcagcgt cgccagcagc
     4501 tcacgcagct tctcgtgaaa ttcacgcagg gtgtgctcca gtcgttcaac cacttcggca
     4561 tcttttatct gaacactcgt ccagtcgcct gctttatcga acagaccaaa ctggtaactg
     4621 taggtaaaac gggattcctg cgcttccagt tccatccacc agccccagaa ctcacgcact
     4681 tccggtgccg gtttcacgtt gacgcataca gccagacaat cgaaaaagaa tcgattatct
     4741 tcgcacttac cttcacgaat atacgggcct agtgcggtaa attttttgat caatctgctc
     4801 ttcgggtgtc cactcggtaa cgtcattgca atctcctgtt gtgatgcaac tgttttacca
     4861 aattggtaaa atttagcaaa tttttaacac aaacgttttt cgatccagtc tgtgatttcc
     4921 tgaaggcctt tgtcaaaatt ccgatacact gggttaaatg ggatctctaa taatttaccg
     4981 tcagcagatg atgaggtgat taagcgtgag tcctcttccg ggctgaacgg atcgttcttc
     5041 cagtagcctg ataacattgg cgttgggcag cgacgtccca gcaatccttg cacttttaat
     5101 gaatagcgat tcaactccac tcgcaacgct tcgtcggaag catcatgcat ccccaaacga
     5161 ctggccagaa cgtcaagata catttccggc acctgttgtt ggcacttaaa atcactcaac
     5221 agggtatgaa ctaccggacc aagacaggca accactttca gacgcggcga ttcaaggtat
     5281 gccagacgca cggcaacgtt agcgccgaaa cggaaaccaa aggccgcgac gcgagtgtga
     5341 tccacccacg gtacgttagg cagcgccttt aaaacgtgct gatgcaacag gctggagtcc
     5401 tgggtgagct tccattttga agaaaagccc accgacggca tatcaatagt cagcatagca
     5461 atgccgcgcg gcgcaaaata acgttcatac aggctgtaat agtccgtctg catcgcatcc
     5521 agaccaccac acattaatac tgtcgggaac gggccatcgc ctttcggcat atgcaaaaag
     5581 ccggtgatgg gcgcaccgcc gggtacggta aactccatct gccgcatcgt gcccggcaga
     5641 cgctgagcgg cctcttcata ggcgcggttt gacaaagcct gcgcttgctc ggccaggtca
     5701 tctcctttca gatgaggata ggcagcaatg ttgtacaacg tagccgcatg cagccagtgt
     5761 cgaccgctga gttgcggatc ttcttccgca caggcttttt gttgccacac catcgcctgg
     5821 gtagcccact cataaatcca gttgccgccg cggtagccaa tcaccgtatc gtataaatca
     5881 tcgtcggtac gttcggcatc gctcatcaca atacgcgcct ggacatcgag gatttcacgt
     5941 gggtcaatgc cgcgccagat ccacatcaga cggttaatca tgcgatacca gtgagggatg
     6001 gttttaccat caagggccga ctgcacaagc agttgtgcgc cgtgactaaa gcggcggact
     6061 agcgtcgagg tttcaggatg tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc
     6121 tgtgtcatgg atgcagcctc cagaatactt actggaaact attgtaaccc gcctgaagtt
     6181 aaaaagaaca acgcccggca gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg
     6241 gacgaatacg acgcccatat cccacggctg ttcgatccag gtattttgcg gaatatcaac
     6301 aacataatca tcaaccagcg gacgaccggc cggttttgcg aagatggtga caaagtgcgc
     6361 ttttggatac atttcacgaa tcgcaaccgc agtaccaccg gtatctacca ggtcatcaat
     6421 aacgatgaag ccttcgccat cgccttctgc gcgtttcagc actttaagct cgcgctggtt
     6481 gtcgtgatcg tagctggaaa tacaaacggt atcgacatga cgaataccca gttcacgcgc
     6541 cagtaacgca cccggtacca gaccgccacg gcttacggca ataatgcctt tccattgttc
     6601 agaaggcatc agtcggcttg cgagtttacg tgcatggatc tgcaacatgt cccaggtgac
     6661 gatgtatttt tcgctcatgt gaagtgtccc agcctgttta tctacggctt aaaaagtgtt
     6721 cgaggggaaa ataggttgcg cgagattata gagatctggc gcactaaaaa ccagtatttc
     6781 acatgagtcc gcgtcttttt acgcactgcc tctccctgac gcgggataaa gtggtattct
     6841 caaacatatc tcgcaagcct gtcttgtgtt gacaacattt tctgctaacc ctgtgacctg
     6901 caatactgtt ttgcgggtga tcgacaagga gacttaacgt gtctgaactg tctcaattat
     6961 ctccacagcc gctgtgggat atttttgcca aaatctgttc tattcctcac ccgtcctatc
     7021 atgaagagca actcgctgaa tacattgttg gttgggcaaa agagaaaggt ttccatgtcg
     7081 aacgcgatca ggtaggtaat atcctgattc gtaaacctgc taccgcaggt atggaaaatc
     7141 gtaaaccggt cgtcttgcag gcccacctcg atatggtgcc gcaaaaaaat aacgacaccg
     7201 tgcatgactt caccaaagat cctatccagc cttatattga tggcgaatgg gttaaagcac
     7261 gcggcaccac gctgggtgcg gataacggca ttggtatggc ctctgcactg gcggttctga
     7321 ctgacgaaaa cgtggttcac ggcccgctgg aagtgctgct gaccatgacc gaagaagccg
     7381 gtatggacgg tgcttttggc ttacagagca actggttgca ggctgatatt ctgattaaca
     7441 ccgactccga agaagaaggt gaaatctaca tgggttgtgc gggaggtatc gacttcacct
     7501 ccaacctgca tttagatcgt gaagcggttc cagctggttt tgaaaccttc aagttaacct
     7561 taaaaggtct gaaaggcggt cactccggcg gtgaaatcca cgttggcctg ggtaatgcca
     7621 acaaactgct ggtgcgcttc ctggcgggtc atgcagaaga actggacctg cgccttattg
     7681 atttcaacgg cggcacactg cgtaacgcca tcccgcgtga agcctttgcg accattgctg
     7741 tcgcagctga taaagtcgac gccctgaaat ctctggtgga tacctatcag gagatcctga
     7801 aaaacgaact ggcagaaaaa gagaagaatc tggcattgct gctggactcc gtagcgaacg
     7861 acaaagctgc attaacggcg acatctcgcg atacctttat tcgtctgctg aacgccaccc
     7921 cgaacggtgt gatccgcaac tccgacgtgg caaaaggtgt ggtcgaaacc tccctgaacg
     7981 tcggtgtggt gaccatgacc gacaataacg tagaaattca ctgcctgatc cgttctctga
     8041 tcgacagcgg taaagactac gtagtgagca tgctggattc gctgggtaaa ctggcaggtg
     8101 cgaaaaccga agcaaaaggc gcatatcctg gctggcagcc ggacgctaac tctccggtaa
     8161 tgcatctggt acgtgaaacc tatcagcgtc tgttcaacaa aacgccgaac atccagatta
     8221 tccacgcggg cctcgaatgt ggtctgttca aaaaaccgta tccggaaatg gacatggtgt
     8281 ctatcgggcc aaccatcacc ggtccacact ccccggatga gcaagttcat atcgaaagcg
     8341 ttggccatta ctggacactg ctgactgaac tgctgaaaga aattccggcg aagtaattat
     8401 ttgatttgct gccggatggc gtttaacgcc ttccggcagt ttcatccttc attatccttc
     8461 gataaaagcc atccctgtaa acgttcgtcg cgggttgcct cgttcaatct ggtgatggaa
     8521 cattcgccgc tgcgatttca gcgccgcgct attttcctgt tgctgttgtt ccagcttcca
     8581 ggcaatcagt aatcgcgcca gccgtttgtt agcatgctga ctgcgctctg actgaacctt
     8641 cacgctaata ccggatgcca gatgcgtggc gcgtaccgcc gagtcgattt tattgacatg
     8701 ttgaccgccc ggccctgacg aacgcagcgt ctcataacgg attgcatccg attgttcctg
     8761 ctcatcagcg gtaaaacgcc caatgcccag aaaccagttt ttgcgcccat gatgaggccg
     8821 atacggactc ggacaaatcc actggatagt gccgcaccag ctttcgctta gagcccatgc
     8881 gttatcgcca tccagagaaa tcagcgccga acgcagtgtg tcagagtagc gacccgtttc
     8941 tgtttccagc accgttaccg cgacgtcctg tcgggtagct tctttaatca gcctgtccag
     9001 tgcttttttc actgcgagac aacattcttc cggcccctga gcagaggaga gttgtagcaa
     9061 gatcatcctt ttttccctcc actgtttttg agcgtcagca ccgggcgcag tcgcgcgaca
     9121 ggaataatta acccagccag caccagacat tgcaccacgc tttcagccga tttataggct
     9181 tgtggcgctt cttcaaagat gagttgttta tcgcgacaaa ttacccgact gccaagttca
     9241 gtccgcgaga gttgcgtcgc cgtgtatttc gctgccagac gccctttaca ctcggtgcgc
     9301 ccccatttac gccccgcccc atgcgccagc gagtggagcg ttttttcgtt cgcaacaggt
     9361 ttaaccagcc aggagtaatc gccgcgtgaa ccgggaataa tcaccagacc gttgtcatcc
     9421 ggcgtggcgc ctttacgatg caaccagccc tgttgatcgc cgatttggca cgcgctaaca
     9481 aagttatgcg ccacatccag aaccggactt ccggtggcct taacttgttg cattatgcgc
     9541 aaagcgatca gctggcgatt aatacgcgca aacgccagcg catcgtcatg ttccgcaata
     9601 taccgtagcg cgtcgtcact gccttcaggc aaaccatgat gcgaaaacga ggcaatatgc
     9661 cgctgtaaaa tagactgccc caatcccctc gagccgctat gaaccagcag ttgcagatgc
     9721 tgcgcatcca gaccagccag tgcaaacagt tccgcgtcga taatttgatc aatctgttgc
     9781 agttctgcga agtggttacc gccaccgatg gagccaagcg agttgcgcca cggatgctgt
     9841 gcaaatgctg acggcaggtt ttcctccagc cagctttctt cagcaacgtc atccagatct
     9901 gataatcgct tttcaaactt atcggcgtta tatttgcgag cgagaatatc tgtttgccat
     9961 agcgccatgc cgcagccgat atcattgccg accagtgccg ggtaaaaacg accaacagag
    10021 aagaacgctg cgccaatcgg gtagccgcgt ccgggatgta aatctggcat ccccacaacg
    10081 cgctgcatgt tgggtaaatt tgccgtggtg tgtaattgtt ggatcgctga actttcgatc
    10141 cacaggtcat cggatgcgat ggtaaatacc gcatcggata agggacgaat atatttgccc
    10201 atgtaccatt ccatttatga ataaaaatca ggaaatggca gacgaatcca gggcaagaaa
    10261 aaaagaatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt gtcatgattt
    10321 acctccttac agtttgtgga ataagaaaac gcgcacatac tagacgggcg ttattttcat
    10381 tgcaagctgg atttaatgtt gcggtttata tcgcatataa aaattagtaa accacgttcc
    10441 ccggcattca acgcgctgct gcttaactgt ctgaaaacca taacgttcaa aaaagggttt
    10501 tgcggttatg ctggcgtcca ccataagttc ggattcagac ttaatcaatg gttttaacaa
    10561 agcgctggca accccgcgtc gggtgtattc cggttcaaca aataacatat cgatataatg
    10621 tccaatgcag gtaataaaac caaccggttg tgcattaata actgcaaccc gcacttgtga
    10681 tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg caatttgctg
    10741 tggagaatag tgctgactcg ccgtcatcgt aacagctcga aggaaaatag cgcatagttg
    10801 ctgaaaatca ccaggctgat agtttcttat ttgtatgtta ttcataatcc cagcaccagt
    10861 tgtctttcca tttgagggtc aagcaacgtc acatgcagcc ccaccagtcg cacaccgcgc
    10921 ccgccgcgac gttcatccca ggttttacgc gcggtggcga ttagatcagc tttattcagc
    10981 cgcggccaga cgtgctcctg agtagtttgc tgaaaatcat caaactttaa tttcaccccc
    11041 tggcgagcaa tcagtaaatc aggttttacc tttgccagac ggcgttcaag ttcaggatac
    11101 agccgctcga taatcgcttc acattcagac cagtgatgaa tatcttccgc catcgtgcgt
    11161 tccacgccga cggattttcg caaccgttca ctgttaacgt cgcgctcgtc aatcccctga
    11221 ctacgctccc acaaaatgcg gccaaatttg ccaaaacgtt tgagcagagt caccagatca
    11281 cacttttgca catcaccgca ggttcgtagc cccatcgctt ccagtttagc cgctgagact
    11341 ttgccgacgc cgggaatttt tgccagcggt aaggtttgca gaaatgccgg aacctcagcc
    11401 ggagtaatca caaactgacc gttgggttta ttcatgtcgg aggcgatttt ggcgagaaac
    11461 tttaccggcg caacgcccgc agatgccgtc agttgcagct cgttaaagat cgtctggcgg
    11521 atttcctggg cgatgagggt cgcggagccg tggcaatgca cactatcggt gacgtcgaga
    11581 taggcttcat ccagtgataa cggttcaatg cgcgaggtat agcgcgagaa aatttcgcgg
    11641 atatgatttg aggcttcttt gtaggcgtca aagcgccctg gaagcagcgt gagatgtggg
    11701 cataatttga gtgccatccc tgtcggcata gcgctacgta cgccaaattt acgcgcggga
    11761 taattggcgg tgctgatcac cccccgacgt tcgcggctgc cgccaatagc aatagggata
    11821 tcgcgcaggg caggattgtc gcgcatctcc accgctgcga aaaagcagtc catatccaca
    11881 tgaatgattt tacgcattgc tcacctctca acactggtaa agtatacagt gatttcaggg
    11941 gttgagaaat gcgtaaagat tcagcattta acgcgctcgc tgcgaaagca cctgctgctt
    12001 atccagcttt tgcaccagcg gctgcaccac tttatcccca tgctgaccaa agtattgata
    12061 cagcgtatcg gacgcacttt tggtcagcac cataatctca atgcgccggt tgcccgcgct
    12121 ttgcggattt ttggcatcca gcagcatctg gtccgccatt gcgcttacct gcatcacttt
    12181 attttccggc attccggcct cttccagcac ccgacgagcc gaaagcgcgc gatcgcccga
    12241 aaggttccag ttgttgtaga tattgttttt gtaggccatc gcatcggtat gcccggtaat
    12301 aataattttg ttatcgagcg agtcgaacac tggcgcaagc tccaccagca aagttttaaa
    12361 gaacggcata atcttcgcgc tgccgcgttc aaacatattg cggttctggt cgtctttaat
    12421 cagcacgcgt aatccctggg gaacaatctc catctccaga ttcgcttcca tatgcgcatc
    12481 acgggcgatg gtattgatac tggtcgccag ctcgccaagc tcggtggcgg atttcttctt
    12541 caacagcgcc gttttctcgt tgacgtcgcg cgcttttttc tccgtctctt ccggcaccgc
    12601 cacggtggca ggttttggca gatgagacgg gctgatttta tttaacggcg acaacccacc
    12661 accgttaaaa atcgactgcc cgtgcagcgc ggcgataatg ctttcacgct cggatttact
    12721 gacgctgttg acaatcaact ttatcgtcgt caccaagggg gccgagcgta tttccgaggt
    12781 ttctgcccgc tttaccctgg acgcgatgcc cggcaaacag atggcgattg acgccgatct
    12841 taacgccggg ttgatcaacc agacgcaggc gcaaacccgg cgtaaagatg tcgccagcga
    12901 ggccgatttc tacggcgcaa tggacggggc atcaaaattt gtgcgcgggg acgccatcgc
    12961 cgggatgatg attctggcga tcaacctgat cggcggcgtc tgtatcggca tcttcaaata
    13021 caacctgagc gccgacgccg ccttccagca atatgtgctg atgactatcg gcgatgggct
    13081 ggtggcgcaa atcccttccc tgctgctctc caccgcggcg gcgattatcg tcacccgcgt
    13141 cagcgataac ggtgatatcg cccatgacgt gcgtaatcag ttgctggcaa gcccgtcggt
    13201 tctctacacc gccactggaa ttatgttcgt gctggcggtg gtgccgggaa tgccgcattt
    13261 cccgttcctg atgttcagcg ccctgcttgg ttttaccggc tggcggatga gcaagcgccc
    13321 gcaagcggcg gaggcggaag agaaaagcct cgaaacgcta acccgtacga tgacagaaac
    13381 cagcgagcaa caggtcagtt gggaaactat tccgctgatc gaacctatca gtttaagcct
    13441 cggctacaaa ctggtggcgc tggtggataa agcgcagggc aacccgctca cccagcgtat
    13501 tcgcggcgta cgtcaggtga tttccgacgg caacggcgtg ctgctgccgg agatccgtat
    13561 tcgggaaaac ttccgcctca agcccagcca gtacgctatt ttcatcaacg gcattaaggc
    13621 tgatgaagcg gatattccgg cggataaact gatggccctg ccctccagcg aaacctacgg
    13681 cgagatagac ggcgtgctgg ggaacgaccc ggcgtacggg atgccggtca cctggattca
    13741 gcccgcgcag aaatcgaagg cgctgaatat ggggtatcag gtgatcgaca gcgccagcgt
    13801 aattgccacc catgtgaata agattgtgcg cagctatatt cctgatttgt ttaactatga
    13861 cgatattact cagttgcata accgtttgtc gtcgatggca ccgcgactgg cggaagattt
    13921 gagcgcggcg ctcaattaca gccagttgct gaaagtgtac cgggcgctac tgactgaagg
    13981 cgtttccctg cgcgatatcg tcaccatcgc caccgtgttg gtcgccagta gtgcggtgac
    14041 taaagatcat attctgctgg cggccgatgt gcgtctggcg ctgcggcgca gcattaccca
    14101 tccgttcgtt cgcaagcagg agctgacggt gtatacgctg aataatgagc tggaaaatct
    14161 gctgaccaac gtggtgaatc aggcgcaaca ggccggcaaa gtgatgcttg atagcgtgcc
    14221 ggtggacccg aatatgctca accagtttca gatcacgatg ccgcaggtga aggagcagat
    14281 gaaagcggcg gggaaagatc cggtgctgct ggtgccgccg cagctgcgcc ctttgctggc
    14341 gcgctacgca aggttgtttg caccggggct gcatgtgctg tcgtataacg aagtgccgga
    14401 tgagctggag ttgaagataa tgggggcgtt gatataggca tccggcatcg gtgcatattg
    14461 ccggatgcgg cgtgaacgcc ttatccggcc tacgggtgag cacggttttg tcggcatgat
    14521 aagacgcgta gcggcgcatc tggcaccggt gcacaactgc cggatgcggc gtgaacgcct
    14581 tatccggcct acgggtgagc gcggttttgt cggcatgata agacgcgtag cggcgcatct
    14641 ggcaccggtg cacaaccgcc ggatgcggcg tgaacgcctt atccggccta cgggtgagtg
    14701 cggttttgtc ggcatgataa gacgcgtagc ggcgcatctg gcaccggtgc acaaccgccg
    14761 gatgcggcgt gaacgcctta tccggcctac gggtgagcgc ggttttgtag gcatgataag
    14821 acgcgtctgc gtcgcatcag gcatcggcgc acagctataa aatgatacgc tccccagcac
    14881 taaaatctgt tacgtctccc gcccaatcga tgggatataa ccctttcgca acatcgcggt
    14941 gaaacgttga gaatggccaa tcactcactt gttttaccca accatgtttt actggattta
    15001 tataaatata atcaacatga tggcgatagt ctttggtatt gcggatagca tgttcccaaa
    15061 aacgtggttg ccagatattt ttcaatccac aagcatgggt aaattgcttt ttaatttccc
    15121 gccagcgcga ggaaaaatca tcatcgcctt caggtaatgt ccatatgcaa tgcatatgct
    15181 ccggcaaaac tacccaagcg ttaatttcaa aaggtctgtc tcgcttaact ttaataataa
    15241 cgttacggag cgtctgaaac tgggtggtca aaagttggtt tcgacgattc tgtaaattta
    15301 ccgtgaaaaa ccaggttccc cccttgatgt aataacgacg atattcagac ataacgactc
    15361 ctttttctca acggtaagaa gaaaaggaaa agtcggcaat gatgttgtgt taaatcggga
    15421 aagcggctcg caaaaaatgc acagatttgt aggcatgata agacgcgtag cgtcgcatca
    15481 gacaccggcg cacaac
//
LOCUS       DAZKHK010000116        15040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKHK010000116 DAZKHK010000000
VERSION     DAZKHK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.116"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(100..2379)
                     /gene="maeB"
                     /locus_tag="ACHOS5_003862"
     CDS             complement(100..2379)
                     /gene="maeB"
                     /locus_tag="ACHOS5_003862"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP9778605.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2668..3618
                     /gene="tal"
                     /locus_tag="ACHOS5_003863"
     CDS             2668..3618
                     /gene="tal"
                     /locus_tag="ACHOS5_003863"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9778606.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPHDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQAQACAEAGVFLISPFVGRIYDWYQARKPIDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3638..5641
                     /gene="tkt"
                     /locus_tag="ACHOS5_003864"
     CDS             3638..5641
                     /gene="tkt"
                     /locus_tag="ACHOS5_003864"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9778607.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWNAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5718..6761)
                     /gene="ypfG"
                     /locus_tag="ACHOS5_003865"
     CDS             complement(5718..6761)
                     /gene="ypfG"
                     /locus_tag="ACHOS5_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP9778608.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSREERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6887..7462)
                     /gene="nudK"
                     /locus_tag="ACHOS5_003866"
     CDS             complement(6887..7462)
                     /gene="nudK"
                     /locus_tag="ACHOS5_003866"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP9778609.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNTKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7530..9509)
                     /gene="aegA"
                     /locus_tag="ACHOS5_003867"
     CDS             complement(7530..9509)
                     /gene="aegA"
                     /locus_tag="ACHOS5_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP9778610.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHYFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAVGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9715..11415
                     /gene="narQ"
                     /locus_tag="ACHOS5_003868"
     CDS             9715..11415
                     /gene="narQ"
                     /locus_tag="ACHOS5_003868"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP9778611.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11579..14692
                     /gene="acrD"
                     /locus_tag="ACHOS5_003869"
     CDS             11579..14692
                     /gene="acrD"
                     /locus_tag="ACHOS5_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP9778612.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14791..14850)
                     /gene="ypfM"
                     /locus_tag="ACHOS5_003870"
     CDS             complement(14791..14850)
                     /gene="ypfM"
                     /locus_tag="ACHOS5_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP9778613.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3525 a         3991 c         3912 g         3612 t
ORIGIN      
        1 tttttattat tcgcaaactc agtaataaac tttactaata tattgcagct aaaccgctta
       61 ccctgaatat tcagggtaag cgtgagagtt aaaaaagaat tacagcggtt gggtttgcgc
      121 ttcaaccacg gccagcgcca ccatgttgac gatacgacgt accgatgcaa tcggcgttaa
      181 cacgtgaacc ggtttcgcca cgcccatcag caccggaccg acagtcacac cttccgagct
      241 ggaaacgcgc agtaagttgt aactaatgcg ggcagcttcc atgttcggca tcaccagaat
      301 attagcggaa cctttcaaag ggctgtccgg catacggtcg ttgcgaatcg cttccaccag
      361 cgctgcatcg ccgtgcattt caccatcaat catcagttct ggcgcacgtt ccctgaccag
      421 ttccagcgcc tgacgcattt tgctcgacga cgggcagtca gaagaaccaa agttggagtg
      481 cgacaacaaa gccacacgcg gctcaatacc aaaacgacga acagtttctg ccgccatcaa
      541 ggtgatctcc gccagctctt ctgcatcagg ctcatcattg acgtaggtat cggcaataaa
      601 ggtgttacca ctcggcaaca gcagcgcgtt cattgcacct gcggtgtgaa cgccatcgcg
      661 ataaccaaag acatttttca ccacgctaaa gtgttcatga taatcgccca ccgtaccgca
      721 aatcattgca tcggcttcgc cacgctgaac catgatcgcg ccgatcactg tcgggttact
      781 gatcagcgca cgctgcgcct gctcctgagt gacaccgcga cgcttcatga tctggaagta
      841 ttcggtccag tactctttaa agcgcggatc ggattcgtta ttgacgatct caaaatcaac
      901 gcccgccttg atctgcaagc ccagtttctg aatgcgcatt tcgatcacgt tcggacgacc
      961 aataaggatc ggtttcgcca gtcccagagt caccagttcc tgagtcgcgt gcagaacgcg
     1021 cgcctcttcc ccttccggca gaactacgcg cttcggcgct ttgcgagcct gggagaaaat
     1081 cggcttcata aacaggttgg ttttgtaaac gaactccgtc agcttgtcga tgtagacgtc
     1141 gaaatcagca atcggacgcg tcgccacgcc cgactccatc gccgctttcg ccaccgcagg
     1201 agcgatctta acgatcaagc gcggatcaaa cggttttgga atgatgtatt ccggaccaaa
     1261 gctcagatcc tgatcgccat acgctgaagc caccacttca ctctgttccg catgggccag
     1321 ttctgcaatt gcacgtactg ccgccagttt catctcttcg ttgatggcgg ttgcgccaac
     1381 gtccagcgcg ccacggaaga taaacgggaa gcaaaggacg ttgttcacct ggttcgggta
     1441 gtcggaacgg ccggtacaga tgatggcatc cggacgcact tctttcgcca gcggcggcag
     1501 aatttccggt tccgggttcg ccagcgccag gatcattggc gcacgggcca ttttcttcac
     1561 catttcctgg gtcagcactt tcgggccgga acagccgagg aaaatatccg cgccttcaat
     1621 cacatcatcg agggtacgtt taccgtcatc caccaccgcg tatgcggctt tggtttccgc
     1681 catgtttggc tcacggccct gatagataac gccttttgaa tcgcaaacca cgatgttatg
     1741 tttttgcaga cccagcgcta ccagcaggtt catacaggcg attgctgcgg cacccgcgcc
     1801 agaaaccacc atccgcacgt cggagatgtt tttctccacc acgcgcaagc cgttgaggat
     1861 ggcggcagtg ctgataattg ccgtgccgtg ctgatcgtcg tggaataccg gaatattcat
     1921 ccgctcgcgc agtttctgtt caatatagaa acattctggc gctttaatgt cttcgaggtt
     1981 gatgccgccg aaggtcggtt cgagcgcagc gacgacttca ataaatttgt ccgggtcgag
     2041 ttcatcaact tcaatgtcaa atacatcaat cccggcgaat ttcttaaaca gaacgccctt
     2101 gccttccatc accggtttgc ctgccagcgc gccaatgttg cctaacccca gcaccgccgt
     2161 accgttagag atcaccgcta ccaggttacc gcgcgcggta tatttgtagg cttttaacgg
     2221 gtctttttcg atttcaagac aaggtgcggc aacgcctggt gagtaggcca gcgccagatc
     2281 gcgctgcgtt gccagaggct tggttggaga aacctggatt ttccctggaa ctggaaattc
     2341 gtggaaatca agtgcacttt gttttaactg gtcatccatt tggttgttcc tttcacgtaa
     2401 cgttcacaaa taaagtgtgt gggcaacagc ccctgtccac aacgtggcgc acattattac
     2461 cctgccggag tctacagact ttgagcaagt ccaaactctc accattaata taatgttttg
     2521 gtaataatcc tataacactg atgttacctg cttaatccag caataccatg cctgtctgct
     2581 atgctttttt gatgcgttta gcgaaatttc tcagaagtgt gaattaacgc actcatctaa
     2641 cactttactt ttcaaggagt atttcctatg aacgagttag acggcatcaa acagttcacc
     2701 actgtcgtgg cagacagcgg cgatattgag tccattcgcc attatcatcc ccatgatgcc
     2761 accaccaatc cttcgctgtt actcaaggct gccggattat cacaatatga gcatttaata
     2821 gacgatgcta tcgcctgggg taaaaaaaat ggcaagaccc aggaacaaca ggtggtcgca
     2881 gcgtgtgaca aactggcggt caatttcggt gctgaaattc tcaaaatcgt acccggtcgc
     2941 gtgtcaacag aagttgatgc acgcctctct tttgataaag aaaagagtat tgagaaggcg
     3001 cgccatctgg tggacttgta tcagcaacaa ggcgttgaga aatcacgcat tctgatcaag
     3061 ctggcttcga cctgggaagg aattcgcgcg gcagaagagc tggaaaaaga aggtattaac
     3121 tgcaacctga cgctgctgtt ttcttttgca caggcgcagg cctgtgcaga agcaggcgtt
     3181 tttctgattt cgccgtttgt cgggcgtatt tatgactggt atcaggcacg caagccgata
     3241 gacccgtatg tggtggaaga agatccaggc gttaaatcag tacgcaatat ctacgactac
     3301 tataagcaac accactatga aaccattgtg atgggcgcga gcttccgtcg caccgaacaa
     3361 atcctcgcct taaccggctg cgaccgactg actatcgcac cgaatttgct gaaggagcta
     3421 caggaaaaag tttcgccagt ggtacgtaaa ttaatcccac cttctcagac gttcccacgc
     3481 ccagctccca tgagcgaagc ggaattccgt tgggagcaca atcaggatgc gatggcggta
     3541 gaaaaactgt ctgaaggcat tcgtctgttc gccgttgatc aacgcaaact ggaagatctt
     3601 cttgccgcca aactataaac cagccacgga gtgttatatg tcccgaaaag accttgccaa
     3661 tgcgattcgc gcactcagta tggatgcggt acaaaaagcc aattctggtc atcccggcgc
     3721 gccgatgggc atggctgata ttgccgaagt gctgtggaac gattttctta aacataaccc
     3781 caccgaccca acctggtatg accgtgaccg ctttattctt tccaacggtc acgcgtcgat
     3841 gctgctctac agtttgctgc atctgaccgg ttacgacctg ccactggaag aactgaagaa
     3901 cttccgtcag ttgcattcga aaacccctgg tcacccggaa atcggctata cgccaggtgt
     3961 tgaaaccacc accggtcctc ttggacaagg tttggcgaac gccgtcgggc tggcgatagc
     4021 agagcgtacg ctggcggcgc agtttaatca gccggatcat gaaattgtcg atcacttcac
     4081 ctatgtgttt atgggcgacg gctgcctgat ggaaggtatc tctcacgaag tctgttcgct
     4141 ggcgggcacg ctggggctgg gcaagctgat tggtttttac gatcacaacg gtatttccat
     4201 cgacggtgaa accgaaggct ggtttaccga cgatacggca aaacgatttg aagcctatca
     4261 ctggcatgtg gtccacgaga tcgacggcca cgatccgcag gcggtgaagg aagcgatcct
     4321 tgaagcgcaa agcgtgaaag ataagccgtc gctgattatc tgccgtacgg tgattggctt
     4381 tggttcgccg aataaagcag gtaaggaaga ggcgcacggc gcaccactgg gggaagaaga
     4441 agtggcgctg gcacggcaaa aactgggctg gcaccatccg ccatttgaga tccctaaaga
     4501 gatttatcac gcctggaatg cccgcgaaaa aggcgaaaaa gcgcagcagc gctggaatga
     4561 gaagtttgcc gcctataaaa aggctcatcc gcaactggca gaagagttta cccgtcggat
     4621 gagcggcggt ttgccgaagg actgggagaa aacgactcag aaatatatca atgagttgca
     4681 ggcgaatccg gcgaaaatcg ctacccgtaa ggcttcgcaa aatacgctta acgcttacgg
     4741 gccgatgctg cctgagttgc tcggcggttc ggcggatctg gctcccagca acctgaccat
     4801 ctggaaaggt tctgtgtcgc tgaaggaaga tccggcgggc aactacattc actacggggt
     4861 gcgtgaattt ggtatgaccg ctatcgccaa cggcatcgcg caccacggcg gctttgtgcc
     4921 gtataccgcg acgttcctga tgtttgttga atacgcccgt aacgccgcgc ggatggcggc
     4981 actgatgaaa gcgcggcaga ttatggttta tacccacgac tcaattggtc tgggcgaaga
     5041 tggtccgacg caccaggctg ttgagcaact ggctagtctg cgcttaacgc caaatttcag
     5101 cacctggcga ccgtgcgatc aggtggaagc ggcggttggc tggaagctgg cggttgagcg
     5161 ccacaacgga ccgacagcac tgatcctctc aaggcagaat ctggcacagg tagaacgaac
     5221 accggatcag gttaaagaga ttgctcgtgg cggttatgtg ctgaaagaca gcggcggtaa
     5281 gccagatatt attctgattg ccaccggttc agagatggaa atcactctgc aagcggcaga
     5341 gaaattagcg ggagaaggtc gcaatgttcg cgtggtttcc ctgccctcga ccgatatttt
     5401 cgatgcccag gatgaggaat atcgcgagtc ggtactgcct tcaaatgttt ctgcccgcgt
     5461 ggcagtagaa gcgggtattg ccgattactg gtacaaatat gttggtctga aaggggcaat
     5521 tgtcgggatg acaggttatg gggaatctgc tccggcggat aagctgttcc cgttctttgg
     5581 ctttaccgcc gagaatattg tggcaaaagc gcataaggta ctgggagtga aaggtgcctg
     5641 atggtgatcg ccggatgctg attgtcggat gtgacgctgg tgcgtcttat ccggtctaca
     5701 tgtccccgcc attttgttta acgggtgatc cacaacgtgg gccaggcgtc tggcccatgc
     5761 cagttatcgc aggtgggttc tgcggcgtaa cgcaccaggc gaaaacgctg accgtcaaag
     5821 cgccagcgcg cctgaatgcc acaatcgctt aatccgcgcc cttttgctaa ggtcaccagt
     5881 tcacgcgatt tttcatcgaa tgttgcgttc atcagttcca gttcattggt ctcctgaccg
     5941 ttgttgaacg gcaaacgcaa ccgtaccggg cgcgaggcca gcggcttttt acgcgacaca
     6001 atccatgcca aatcaatggt gttataggcc cctgcctcac agctaatcat catcagcgct
     6061 ttatcatcgg tcagcgcagt gacattcacc tcacgacgta acggatcaag cgaacagcgc
     6121 agaccattca tccgccagtt tccataatcc agcaaatcgt tacgttcttc gcgtgagagt
     6181 ggcgtcggcg ttgggttaac caccgcgacc tctttcagcg caggcgctgg cggtacgctg
     6241 agcggcggtt cgtccccttt ctttatccac gcggtctcac tgccaacgcg cttttgctgg
     6301 gcatcaataa acaacaacgc cgcttttaag ccactcagag aaatagtctg attgccgtcg
     6361 cgtaaggtga tcgccctccc ttcctgaatc atctgcaaaa acgcggtgat ggttgccgta
     6421 tcatcggtta ctaataacca tggtgaaatc cgccacttgt caccacttaa cgccaacggc
     6481 tcaccatcta acagcagccg tggcgctatc tccccttctg acgcatccgg cgacttcaac
     6541 ccgccgcgct caatacgtaa aacggcatcg gtatgcgccc cggcgctgcg gctcagggtc
     6601 atcaccagtc cattatgatc gcccgtattg cgcgctacac agaaattttg attattacag
     6661 gtgacctgcc agtcggaaaa cgcccgttgc gctggtgccg cccacaccaa agacatcggc
     6721 aacaaagcga aaaaaaagag aaggaaaatg cgatatcgca tggacggtac gaccccagaa
     6781 gttcaaacaa acagcacaag tcgtatcttc gttgcgcagg tagcgcggct caatcggatt
     6841 tgtcggattg agccaataat aaacaacgaa atattattgt tatttttcag tccattaaat
     6901 gtgacatttg caaatagtta agcaataaca ccgtcttacc gtcacgtatc tcgccggttt
     6961 taatcatctc cagcgcctgg ctgaacggca gctcaagcac ttcaatatct tcatcttcga
     7021 caccaccacc ggcgttagcg cgctggctgt cactgtattc ggcgataaaa aagtggatta
     7081 gctcagtcac accgcctggc gacatataca gttcaaataa tttgcggact tcgccaactt
     7141 catagcccgt ctcttcaatc gcttccttgc gaatgcacac ttccggttcg tcgttatcca
     7201 gcagcccggc gcaggtttca atcagttgcc cgctttcatt gccattaacc caggtagcga
     7261 cgcggaactg gcgaatcaga accacggtct ttttcttcgt gttgtacagg aggatcgtcg
     7321 caccattacc gcgatcgtaa acttcacgtt tatggcggat aacttcgcca tctttgcgcg
     7381 tgagatcgta agtaatgttg tgcagggtga aatagttatc ggagagaatt ttgtctttaa
     7441 cgagggtgat ttgttgcgtc ataccgactc cacagcgcga aatgaacaat tatcttacgc
     7501 tgtgaagtcg ggtttgtctg cgcaggctat cagtgagatt tgactgattt tacgcccagc
     7561 cagtcaatga tcccctgtgc cgcatgacgt ccttctgcca ttgcggtaac caccagatcc
     7621 gcaccacgca cggcgtcacc accggcgaag attttcggat tcgtggtctg gtaacggtac
     7681 tggctttcca catccgcgat gatgcggccc catttgtcta ccgttacacc gtgcgactcc
     7741 agccacggca tcccgtgcgg attgaagcca aacgccataa tcaccgcatc ggctggcatg
     7801 acaaattcac tgccttccac cggcactgga cgccgacgcc cctgggcatc cggctcgcca
     7861 agacgcgtgc gcaggaaacg aatcccgcag acgtgacctt gttcattcag ttcaagcgcc
     7921 accggctgga cgttaaattc gaagttggcc ccctcttcac gggcgttctt cacctctttc
     7981 ttcgagcctg gcatgttagc ttcatcacga cgataagcgc aggtgacgtt actcgcgccg
     8041 tggcgcagcg cagtacgcac acagtccatc gcggtgtcgc cgccgcccag taccacgacg
     8101 ttaagtccgg cagtattgat aaacggctct tccggtagct cttcgagtcc catcacctgt
     8161 ttagtattgg cgatgaggaa cggcagcgcg tcataaacgc ccggcgcatc ttcattgggt
     8221 aaacccgctt tcatggaacg gtaagtgcct acgccaacga agaccgcgtc gtattgttcc
     8281 aaaagcgaat tcaaagagac atctttaccc acttcacaat tgagttcgaa gtgaatcccc
     8341 atcgcgctga agatttcccg acggcgtgcc agcagggatt tatccagttt gaaagaagga
     8401 atgccgaaag tgagcaagcc accgatttct ggatggcgat cgtacaccgt cacctctacg
     8461 ccattgcggg tcaaaacatc cgcacaggcc agccctgccg gacccgcacc gataatcgcc
     8521 acccgcttgt ccactttggt gacatggctt aagtcaggac gccaaccttt cgccaacgcc
     8581 tgatctgaaa tgtagcgttc aatgttaccg atagttactg cgccgtgctc atcgcgaata
     8641 gtacaggcac cttcacacaa acggtcttgc gggcaaacgc gtccggtaat ttccggcagg
     8701 gtgttggtct ggtgagaaag ctcgacggcg gcgtcgatgt ttccggcttt caccagttca
     8761 atccactgcg gtatatggtt atgcagcggg caggtccatt cacaaacgct gtgctcgccg
     8821 cacttaaggc agcgcgaggc ttcccgttgt gcctgatcgg cacggaatgg cagataaatt
     8881 tcatcaaaac cggttttgcg cgcttcaatc gccagtttat ctggctcgcc acgcgcgggc
     8941 gttgcctgca tttgctcgac tttactcatt accggcattt cttgcgccgc ggtactggca
     9001 tgccacggtt gttgttcctg acgtgcggtg cgcaagcggc gggatttcgc catgccggag
     9061 agtgcggcgt cagtgaccag ttgcagcgcg tccgccgggc aattttcgac acaggcagga
     9121 ccgttttcgc gccctgcaca aaggtcgcat ttatgcgccg tggcttttac ttttcctacc
     9181 gcaacgggtg tcagaacgat ttgcatcgta ccaaatggac aggccaccac gcaggattta
     9241 cagccaatac acttttgctg attgacctga atgctgtcat caacgtggct gattgcgcca
     9301 ttagggcagc tacgggcgca gggcgcatct tcacaatggt gacaggtcac tgcactacgt
     9361 tgctgctgat gtttgataac cgtaattcgg ggatgaaaat agtgttggct caggacatgt
     9421 tgctcgtcat tgtgagccat gacacaggcg atttcacaag catgacaacc cagacattgc
     9481 tgactgttgg ccataataaa acgattcata acgaccttct tttttggttg taaaaacctt
     9541 attctttata tgagtgttgt tattacccga cttacagggg aatcggcaat gttcatgtgc
     9601 ccagaataag taactatttc gctataaact gtggcagatc aaataatccc atcactgact
     9661 aaatttcgtt tcagcgaact ggaacattaa tgattttttg tggagaagac gcgtgtgatt
     9721 gttaaacgac ccgtctcggc cagtctggcc cgggcctttt tttacattgt gctgctgtcg
     9781 attctttcca cgggtatcgc tctgctaacg ctggcgagca gtttacgcga cgctgaggct
     9841 atcaatattg ccggatcgct gcgtatgcag agttaccgcc tgggctacga cttgcaaagt
     9901 ggcagtccac aactcaatgc acatcgccag ttatttcagc aggcactgca ttcaccggta
     9961 ttaaccaatc tcaacgtctg gtatgtgcca gaggcagtaa aaacccgcta tgcgcatctg
    10021 aatgccaact ggctggagat gaataatcgg ctcagcaagg gcgacttgcc gtggtatcag
    10081 gccaatatta ataattatgt taatcagata gacctgttcg tgctggcttt acagcactac
    10141 gctgaacgca aaatgctgct ggtggtggca atttccctgg ctggcggcat cggtattttc
    10201 acgctggtct tttttacttt gcgccgcata cgccatcagg tggttgcccc gctgaatcag
    10261 ctggttaccg ccagtcagcg tattgaacac gggcagttcg actcgccgcc gctggatacc
    10321 agcctgccga atgagcttgg cctgcttgca aaaaccttta accagatgtc gagcgagctg
    10381 cataaattgt accgttcgct ggaggcgtca gtagaagaaa agacccgcga tctccacgag
    10441 gccaagcgtc gtctggaggt gttgtatcag tgttcgcagg cgctaaacac cagccagatt
    10501 gatgtgcatt gtttccgcca tattttgcag attgttcgcg acaatgaagc ggctgaatat
    10561 ctggagttaa atgtcggtga caactggcgg attagcgaag ggcagccaaa cccggaattg
    10621 ccgatgcaga ttttaccggt gacaatgcaa gagacggttt acggcgaact gcactggcaa
    10681 aatagtcacg tttcatcatc agaaccgctg cttaacagcg tttcgtcgat gctgggacgc
    10741 ggtttgtact ttaatcaggc gcagaagcat tttcagcagt tattgttgat ggaagaacgt
    10801 gcgaccatcg cccgcgaatt gcacgactcg ctggctcagg tactttctta cttacgtatc
    10861 cagttgacgt tactgaagcg ttcgataccg gaagataatg ccaccgcaca aagtatcatg
    10921 gccgattttt cccaggcgtt gaatgatgct tatcggcagt tacgcgagct gttgactact
    10981 ttccgcctga cgctgcagca ggcggatctc ccctccgcgt tgagggaaat gctggatacg
    11041 ttacaaaatc aaaccagcgc caaactgacc ctcgactgcc gtctgccaac cctggcgctg
    11101 gatgcgcaaa tgcaggtgca tttgttgcaa attattcgcg aagcggtgct gaatgcgatg
    11161 aagcacgcca acgccagcga aatcgccgtt agctgcgtca ccgcgccgga tggcaatcac
    11221 acagtctata ttcgcgacaa cgggattggc atcggtgaac cgaaagaacc cgaaggccat
    11281 tatggtctga atatcatgcg cgaacgcgcg gaaagattgg gtgggacgct gactttttca
    11341 caaccttccg gcggcggcac gttagtgagt attagctttc gctctgcgga gggtgaggaa
    11401 agtcagctga tgtaatgcct cttattgacc aaagaatagt ggcacttaag gttcagtata
    11461 aaagggcatg ataatttaca ttaactcctt tttttctcca cgattggctc gtaccttgcc
    11521 gctacagtga agcaatccaa gcctacaacg atacgcagaa acacgaggtc ctcttttaat
    11581 ggcgaatttc tttattgatc gccccatttt tgcctgggtg ctggcaatcc tgttgtgtct
    11641 gacaggtacc ctggcgattt tttcattgcc cgttgaacaa taccccgatc tcgcgccacc
    11701 gaatgtgcga gtgaccgcta actatcccgg cgcatcggcc cagacgctgg aaaacaccgt
    11761 gacccaggtt atcgagcaaa atatgaccgg cctcgataat ctgatgtata tgtcatctca
    11821 gagcagcggc accggtcagg catctgtcac gttaagtttt aaagcaggca ccgatccgga
    11881 cgaagccgtg cagcaagtac aaaaccagct gcaatcagcc atgcgaaagt taccgcaggc
    11941 ggtgcaaaat cagggcgtga cggtgcgtaa aaccggcgat accaacattc tgaccattgc
    12001 cttcgtctct accgatggtt cgatggataa acaggatatt gccgattatg ttgccagtaa
    12061 tattcaggac ccgttaagcc gcgtgaatgg cgtcggggat atcgatgcct atggttcgca
    12121 atattccatg cgtatctggc tggacccggc gaaactcaac agtttccaga tgacggctaa
    12181 agatgtcacc gatgccattg aatcacagaa cgcgcagatt gcggttgggc aacttggtgg
    12241 tacgccttcc gtcgataagc aggcgctcaa cgccaccatt aacgcccagt cactgctgca
    12301 aacaccagaa cagttccgcg atatcacctt gcgggtgaat caggacggct cagaggtaag
    12361 gctgggcgat gtcgccaccg tcgaaatggg ggcggagaaa tacgattatc ttagccgctt
    12421 caatggtaag ccagcctccg ggctgggggt taaactggcc tccggcgcga acgaaatggc
    12481 gacagcggag ctggtgctca atcgtctcga cgagctggcg cagtattttc cacacggact
    12541 ggaatacaag gtggcgtatg aaaccacctc gtttgttaaa gcctccattg aagacgtggt
    12601 gaaaacgctt ctggaagcta tcgccctggt tttcctcgtt atgtatctgt tcctgcaaaa
    12661 cttccgcgcc acgctgatac ccactatcgc cgtgccagtg gtgttgatgg gaaccttctc
    12721 cgtgctttac gccttcggtt acagtgtcaa caccttaacc atgttcgcga tggtgctggc
    12781 gatcggtctg ctggtggatg atgccatcgt ggtggtggaa aacgtcgaac gtattatgag
    12841 tgaggaagga ctcactcctc gcgaagccac acgtaaatcg atggggcaga tccagggggc
    12901 actggtcggg attgcgatgg tgctttcggc ggtatttgtg ccaatggcct tcttcggcgg
    12961 caccaccggt gccatctatc gccagttctc tattaccatt gttgcggcga tggtgctgtc
    13021 agtactggta gcgatgatcc tcactcctgc tctgtgtgcc acgctactta agccactgaa
    13081 aaaaggtgag catcacgggc aaaaaggctt ttttgcctgg tttaaccaga tgtttaaccg
    13141 caacgccgaa cgctacgaaa aaggggtggc gaaaattctc caccgtagcc tgcgctggat
    13201 tgtgatttat gtcctgctgc ttggcggcat ggtgttcctg ttcctgcgtt tgccgacgtc
    13261 gttcttaccg ctggaagacc gtggcatgtt tactacctcg gtacagttgc ccagcggttc
    13321 aacccagcaa cagaccctga aagtcgttga gcaaatcgag aaatattact tcacccatga
    13381 aaaagacaac atcatgtcgg tgtttgccac cgtgggttct ggccctgggg gtaacgggca
    13441 aaacgtggcg cgaatgttta tccgcctgaa agactggagc gaacgcgaca gtaagaccgg
    13501 cacctcgttt gccattatcg agcgtgcaac gaaggcgttt aacaagatta aagaagctcg
    13561 cgttatcgcc agcagcccgc cagcaattag cggtcttggt agttctgcgg gttttgatat
    13621 ggagttgcag gaccacgctg gagcgggtca cgatgcgctg atggcagcac gaaaccagtt
    13681 gctggcgctg gcagcggaaa acccggagct aacccgtgtg cgccataacg gcctcgacga
    13741 cagtccgcag ttgcagattg atatcgacca gcgtaaagct caggcgctgg gcgttgctat
    13801 cgacgatatt aacgacacac tgcaaaccgc ctggggttcg agctatgtga atgactttat
    13861 ggatcgcggt cgcgtgaaga aagtctatgt gcaggcagct gcgccgtatc gcatgctgcc
    13921 agatgacatc aatctctggt atgtccgaaa taaagatggc ggcatggtgc ccttctctgc
    13981 tttcgcgacc tcacgctggg agacaggctc gccgcgtctg gaacgctata acggttattc
    14041 tgcggttgag attgttgggg aagccgcacc gggggtcagt accggtacgg cgatggatat
    14101 tatggaatcg ttagtgaagc agttgccaaa cggctttggt ctggagtgga cggcgatgtc
    14161 gtatcaggag cggctttccg gcgcgcaggc tccggcgctg tacgccattt ccttgctggt
    14221 ggtattcctg tgtctggctg cgctgtatga aagctggtcg gtgccgttct cggtaatgct
    14281 ggtcgtaccg ctgggggtta tcggcgcgct gctggcaacc tggatgcgcg ggctggaaaa
    14341 cgatgtttac ttccaggtgg gcctgttaac ggtcattggt ttatcggcga aaaacgctat
    14401 cctgatcgtc gagtttgcta acgagatgaa ccaaaaaggc cacgacctgt ttgaagcgac
    14461 gctccacgcc tgccgtcagc gtttacgccc gattctgatg acctcgctgg catttatctt
    14521 cggcgtattg ccaatggcaa ccagcacagg tgccggttcc ggtggtcagc atgcggtggg
    14581 tactggcgta atgggcggga tgatttcggc cactattctg gctatttact tcgtgccgct
    14641 gttctttgtg ctggtgcgcc gccgcttccc gctgaagccg cgcccggaat aagcaataaa
    14701 aaaggcgaca tgccaatgtg tcgccctttt caactttccg attaagaacc tgctcagcgg
    14761 attcttgctg tttgtacttt gtctcaggaa ttacttacga agcataactt cgataaagtc
    14821 tttccagttc cccagttcac gttcaatcat aacaacctct cttataatta tgggtattct
    14881 acggaaacaa tataccgtgg tgaagctaat ttactcgatt gctgtgatga ctacctccgg
    14941 gggacaaacc ttatgtaaat actatggtcc tgcggtgatg atttgtatgt gatgcaccgc
    15001 aacatttcga gatattcata cggcatctaa tactgattta
//
LOCUS       DAZKHK010000117        15015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKHK010000117 DAZKHK010000000
VERSION     DAZKHK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.117"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(138..1190)
                     /gene="fbaB"
                     /locus_tag="ACHOS5_003871"
     CDS             complement(138..1190)
                     /gene="fbaB"
                     /locus_tag="ACHOS5_003871"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP9778614.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1447..2724
                     /locus_tag="ACHOS5_003872"
     CDS             1447..2724
                     /locus_tag="ACHOS5_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP9778615.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2721..3725
                     /locus_tag="ACHOS5_003873"
     CDS             2721..3725
                     /locus_tag="ACHOS5_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9778616.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRISPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3722..4687
                     /locus_tag="ACHOS5_003874"
     CDS             3722..4687
                     /locus_tag="ACHOS5_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9778617.1"
                     /translation="MSGARLHTLLPELATRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4661..5407)
                     /locus_tag="ACHOS5_003875"
     CDS             complement(4661..5407)
                     /locus_tag="ACHOS5_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9778618.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5459..6277)
                     /locus_tag="ACHOS5_003876"
     CDS             complement(5459..6277)
                     /locus_tag="ACHOS5_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP9778619.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(6342..7142)
                     /gene="thiD"
                     /locus_tag="ACHOS5_003877"
     CDS             complement(6342..7142)
                     /gene="thiD"
                     /locus_tag="ACHOS5_003877"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9778620.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPHVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7139..7927)
                     /gene="thiM"
                     /locus_tag="ACHOS5_003878"
     CDS             complement(7139..7927)
                     /gene="thiM"
                     /locus_tag="ACHOS5_003878"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP9778621.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(7962..8058)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8150..8422)
                     /gene="rcnR"
                     /locus_tag="ACHOS5_003879"
     CDS             complement(8150..8422)
                     /gene="rcnR"
                     /locus_tag="ACHOS5_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP9778622.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8543..9367
                     /gene="rcnA"
                     /locus_tag="ACHOS5_003880"
     CDS             8543..9367
                     /gene="rcnA"
                     /locus_tag="ACHOS5_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP9778623.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9586..9924
                     /gene="rcnB"
                     /locus_tag="ACHOS5_003881"
     CDS             9586..9924
                     /gene="rcnB"
                     /locus_tag="ACHOS5_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP9778624.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10006..11040)
                     /locus_tag="ACHOS5_003882"
     CDS             complement(10006..11040)
                     /locus_tag="ACHOS5_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP9778625.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(11056..13536)
                     /locus_tag="ACHOS5_003883"
     CDS             complement(11056..13536)
                     /locus_tag="ACHOS5_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9778626.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVLAGYQYGFNNLLKLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAAGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(13552..14226)
                     /locus_tag="ACHOS5_003884"
     CDS             complement(13552..14226)
                     /locus_tag="ACHOS5_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9778627.1"
                     /translation="MKGLLALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQLPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNKIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(14307..14849)
                     /locus_tag="ACHOS5_003885"
     CDS             complement(14307..14849)
                     /locus_tag="ACHOS5_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778628.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
BASE COUNT         3720 a         3781 c         3489 g         4025 t
ORIGIN      
        1 gaaaattatt cgaggaatga aaggcaaaaa aaacggcctc ccgggggaag ccgtaacaaa
       61 gtacgcgtgg ttttatgccg gatgtggcgt taacgccttt tccggcctac ggggtagtgc
      121 tgttcgtatg aagcgcatca ggcgatagta attttgctat cgagataaac gtcctgcacg
      181 gcgttaatca gtttcacgcc gtcagccatc gatttcttga acgccttacg tccaagaatc
      241 agccccattc cgcctgcgcg tttgttgata accgcagtac gcactgcatc gctgaggtca
      301 gtttcaccgc ctgcagcacc gccggagttt atcaacccgg cccggcccat atagcagtta
      361 gctaactgat aacgcaccag gtcaatcggg ttttcactgg tcagtttgct gtagactcga
      421 tcgtcggtat aaccgtaatt aatcgcttta tagccgccgt tattttccgc cattttttgt
      481 ttgacgatat ctgcacctat ggttgccgcc agatggttcg cctgaccagt caggtcggcg
      541 gaaacatggt aatcaacgcc atctttctta aaggcggagt tacgcaaata ggcccacagc
      601 actgtcacca tgcccagctc gtgcgcacgt tcaaaagccg cagaaatttc ttcaatctgg
      661 cgacgtgact cttccgagcc aaaatagata gtcgcaccaa ccgccaccgc gcccatattg
      721 aacgcctgct ccacgctggc atacagcgtt tgatcgtagg tattcgggta acttagcgtc
      781 tcgttgtgat taagtttgac gaggaatgga atgcgatgcg cgtaacgccg cgataccgat
      841 gccagcacgc cgtaagttga cgccacacag ttacagcccg cttcgatcgc cagttcaaca
      901 atgtttttcg ggtcaaagta gagcgggtta gcggcaaatg aagctccggc agagtgctca
      961 acgccctggt caaccggcag aatagaaaga tatcctgtgc cagccagacg cccggtgttg
     1021 tacaacgtct gcatattacg taacaccgct ggcgggcgat tattgtcaat catcacgcgg
     1081 tctacgtagt catgtccggg gagataaagc tggtcagaag gaatagtcat acaacggtgc
     1141 tgtaaaaggt tgtcggcgtc tttgccaagc aactgcgcaa tatctgtcat tactatgctc
     1201 ccgtaaattc cgattggata tcggctatgg attgtcctgg cccgcctttt gcgggcaatc
     1261 ataatcctgg tcgttacggg cgagtttttc catcttttgg atgttttttc agcgttttct
     1321 gttggctcga ttcatcagaa aaaatgttac cgcggtcaag ttttcaccgc aaaggtattt
     1381 aaaaggtatt attaagtggt attgtcatcg cgtaccttac attacctgtc atgaaggaat
     1441 taaaagatga aaacaacagc aaagctgtcg ttcatgatgt ttgttgaatg gtttatctgg
     1501 ggcgcgtggt ttgtgccatt gtggttgtgg ttaagtaaaa gcggttttag tgccggagaa
     1561 attggctggt cgtatgcctg taccgccatt gcggcgatcc tgtcgccgat tctggttggc
     1621 tccatcactg accgcttttt ctcggcgcaa aaagtgctgg cggtattgat gttcgctggt
     1681 gcggtgctga tgtatttcgc cgcgcaacag accacttttg ccgggttctt cccgttactg
     1741 ctggcctact cgctaaccta tatgccgacc attgcgctga ctaacagtat cgcttttgcc
     1801 aacgtgccgg atgtggagcg tgatttcccg cgcattcgtg tgatgggcac tatcggctgg
     1861 attgcctctg gtctggcatg tggtttcttg ccgcaaatgc tggggtatgc cgatatctca
     1921 ccgacgaaca tcccgctgct gattactgcc ggaagttctg ctctgcttgg tgtgtttgca
     1981 tttttcctgc ccgacacacc gcccaaaagc accggcaaaa tggatattaa agtcatgctc
     2041 ggcctggatg cgctgatcct gctgcgcgat aagaacttcc tcgtcttttt cttctgttca
     2101 ttcctgtttg cgatgccact ggcgttctat tacatctttg ccaacggtta tctgaccgaa
     2161 gttggcatga aaaacgccac tggctggatg acgctcggcc agttctctga aatcttcttt
     2221 atgctggcat tgccgttttt caccaaacgc tttggtatca aaaaggtatt attgcttggt
     2281 ctggtcaccg ccgcgatccg ttatggcttc tttatttacg gcagtgcgga tgaatatttc
     2341 acttacgctc tcttgttcct cggcattttg ctgcacggcg taagttacga tttttactac
     2401 gttaccgctt acatctatgt cgataaaaaa gcccccgtgc atatgcgtac tgccgcgcag
     2461 gggctgatca cgctctgctg ccagggcttc ggcagtttgc tcggctatcg tcttggcggt
     2521 gtgatgatgg aaaagatgtt cgcttatcag gaaccggtaa acggactgac tttcaactgg
     2581 gccgggatgt ggactttcgg cgcggtgatg attgccatta tcgccgtgct gttcatgatt
     2641 tttttccgcg aatccgacaa cgaaattacg gctatcaagg tcgatgatcg cgatattgcg
     2701 ttgacacaag gggaagttaa atgaaaacag aacgtattct cggtgctctt tatgggcagg
     2761 cgttagggga tgcgatgggg atgccctccg agctttggcc gcgcagtcgc gtcaaagcac
     2821 actttggctg gattgaccgt tttctgcctg ggccaaagga gaataacgca gcctgttatt
     2881 ttaaccgcgc cgaattcacc gacgatacct cgatggcgct gtgtctggcg gatgcgttac
     2941 tggaacgtga aggcaagatc gatccggatc tgattgggcg taatattctc gactgggcgc
     3001 tgcgtttcga cgcctttaac aaaaacgtac taggtccgac ctcgaagatt gcgcttaacg
     3061 ccattcgcga cggtaaaccc gttgctgaac tggaaaataa cggcgtgacc aacggcgcag
     3121 cgatgcgcat ctcgccatta ggttgtttgc ttccggcgcg tgatgttgat tcctttattg
     3181 atgatgtggc gctggcctcc agcccgacac ataaatccga tctggcggtt gcgggggcgg
     3241 tagtcatcgc atgggcgatt tctcgtgcca ttgacggaga aagctggtca gcgattgttg
     3301 attcactgcc ttcaattgcg cgacatgctc aacaaaaacg catcactacc ttcagcgcct
     3361 cactggcagc acgtctggag attgcgctga aaattgtgcg caatgctgac ggcaccgaat
     3421 ccgccagcga acagctttac caggtcgttg gcgcaggtac cagtactatt gagtccgttc
     3481 cgtgcgccat tgcgctggtt gaactggcac aaaccgaccc gaaccgttgc gccgtcctgt
     3541 gcgctaacct tggcggcgac acagacacca tcggtgctat ggcgacggca atttgcggcg
     3601 cgttgcatgg cgttaacgct atcgatcctg cgttaaaggc ggaactggat gcagtaaatc
     3661 agcttgattt caaccgctat gccacggcgc tggcgaagta tcgccaacaa cgggaggcga
     3721 tatgagcggc gctcgattac acacgctgct gcctgaatta gccacgcgtc agccggtgat
     3781 ggtcgtcggc gcggcggtca ttgatgtgat cgccgacgct tatgccctcc cctggcgtgg
     3841 gtgcgatatc gaactgaaac agcagagcgt taacgttggc ggctgcgccc tgaatattgc
     3901 cgtggcatta aagcgcctcg gcatcgaagc gggtaatgcc ttgccgctcg gtcagggggt
     3961 gtgggcggag attattcgca accggatggc aaaagaggac ttaatcagcc tgatcgataa
     4021 cgccgaaggt gataacggct ggtgtctggc gctggttgag cctgatggcg aacgcacttt
     4081 tatgtcattc agcggtgtgg aaaatcagtg gaatcgccag tggctggcgc gattaaccgt
     4141 tgcgcctggc agcctgctct atttttccgg ttatcaactg gcctcgccct gcggcgaatt
     4201 gttagtggaa tggctggaaa agctgcaaga cgtgacgccg tttatcgatt ttggcccacg
     4261 tattggcgat atcccggatg cattactggc gcggatcatg gcctgtcgac ctttagtgtc
     4321 actcaatcgt caagaggctg agattgccgc cgaacgtttt gctttatccg cagagataac
     4381 aacacttggc aagcaatggc aggagaaatt tgccgcgccg ttgatcgttc gtctcgataa
     4441 agagggcgca tggtatttca gcaacgacgc ttctggctgc attccggcat ttccaacgca
     4501 agttgtagac accattgggg cgggcgacag tcatgccggc ggcgtacttg ccgggctggc
     4561 ctctggtctg ccactggcgg atgccgtatt actgggcaat gcagtggcgt cgtgggttgt
     4621 cgggcatcgg ggcggtgatt gtgcgccaac gcgcgaggaa ctactcctcg cacacaaaaa
     4681 cgtatagatc gctgcgacag tggctgatgc tgtactcaat aggccgttgc tgttggtcaa
     4741 gcgccacttg cttgatcacc agcaccggta ttttgctatc taactgaata tgtgactgaa
     4801 actcggcatc cggcatccgg gcgctaacgc gggaacgcgt acgttgtggg taaatgtgct
     4861 ggctgcggaa gtagtcataa agagaaatcc cgatggcatc aacatcatga attaaatgtg
     4921 ccggaaccca cgattcctca atcgataccg cctcttcatc gacatagcga atacgcttaa
     4981 gcaaaaagac atcacttccc gcttcgaccg ccagctgctg cgcgacttct tcgggacatt
     5041 tcacgacacg cttgttaacc cataacgtat cgggcttttt cccgcgcaat accacctgct
     5101 gagaaaaccc ccgcgcttct ttcagcgagt attcgaagat gttgttgatc tgcgtgccgt
     5161 aaccacgcga acgcgtcacc acgccctctt cttccagcga ctgcatcgcc ttgcgcaccg
     5221 taatgcgcga aacgccggtt aactgactta aatcacgctc accgggcaaa atattgccat
     5281 gctccagcac cccgctgcgc acggcatttt ttaccgtttc ggcaaactta atataaagcg
     5341 gcgtgttatc cgccgctaaa atacgctcgt tcagttgggc aatcagctgg gtatgcgctt
     5401 gttccatttg tctttttcct gccggggaat ctggtgggag tatatcactg cttcttattt
     5461 aaggcgtttc tttaatccca tcaacgaatc cctgcaactc ctccaccgtg ccattaaaca
     5521 cattaaaatc caccggacca ttaatgccat cgacctgtcc acggtcggaa tgctgccaga
     5581 agcgccaggc catgccgtca ttgtccgggc gacgttgata atagtgagcc acccaccacg
     5641 gatattcatt gaaatagccc gccagattgg tgtgataaaa aacggctcct gagtaaataa
     5701 ttggcttttt acccgtacgt ttttcgacca tttttagcca ctgacttacc cgcttgcgta
     5761 attcttttgc cgataatttt ccccgttctt ctacgtccag cacggcaggg aaatcgcctt
     5821 gtgagaaatc caccgtttgc agaaataatc tcgcctgaac tgaagcagat accgacgggg
     5881 aaaaataatg atacgccccg cgcagcaggc cattttcgcg gcttagttgc cagttacgcg
     5941 aaaaataggg gtccaccagc ttttcgcctt ccgtcgcctt aataaaagca aactgtaagc
     6001 ggataccgtt gtcgcgcatt tttgccacac gctgccagtc gatccgctcc tgccagcgtg
     6061 aaacatctat tccgtgaatg gtgtaactgg caggaatgcg aatagcgaaa gatttaaccg
     6121 ggcgataacc gtagaagtgg atgtagtctt taatctgaat tgctgtcgaa tagaaaaaat
     6181 tcaccgtctg acgcggataa attgcaacga tggaaattag cccaagagcg cataataaaa
     6241 cggtgaattt ttttcgactg gtgattctca actgcattgg tttcatccct gaatgtcagc
     6301 accaaaggct gacaataacc aaagcagcta tagtacggtg ctcaccacca ggcgtggaag
     6361 tggtgaaccg gaccaatacc gtgaccaact tccagcgtgt cggcctgggc taatgccgat
     6421 gaaagccagc tttttgcctc ctgtacggtg tcagcccagt ttgtatggcg cgggcgtagt
     6481 gcagccaacg ccgcagagag cgtacaacca gtgccgtggg tgtttttggt cataatgcgc
     6541 ggtgcagtaa atcgttgttc cccctcacga gtaaacagcc agtccgggct ttgctcatca
     6601 tccagatgac cacctttcat tagcactgcg ccacagccca tcgccaacag cgatcgccct
     6661 tgttccagca tttcctgttc ggtgcgcgcg tgtggcgcgt cgagcaaggc ggctgcttcg
     6721 ggcaagtttg gcgttattaa tgaaacatgt ggcaataatc gactgcgcag cgtagcaacc
     6781 gccgatggtg aaagcagcgg gtcgccgctt tttgccagca taacggtgtc gagtaccacg
     6841 ttttggatct gataacgttg caaccgttct gccaccgctt caacaatatc ggtttccgcc
     6901 aacataccga ttttagtggt gtcgattcgc acatcactga acaccgaatc gagctgggcg
     6961 gcgacaaaat caggctcaat gcgatacacc gactgtacac cacgggtatt ttgcgccacc
     7021 agtgcagtaa taactgagca accataagcg ccaagtgccg agaaggtttt aagatcggcc
     7081 tgaatccccg caccaccact cggatcagta ccggcaatcg tcagagcgtt aattcgtttc
     7141 atgcctgcac ctcctgcgtc aattgccaga gcgcatcaag gaaatgtgga acaaaactgc
     7201 ctggcccctc gcttctggca actgcgcgtt ctccggcttg tttcatccag tgacaggcag
     7261 atgcgatatt ttccagcgta tcgcctggta gcgcacaaca ggcagcgaca accgccgata
     7321 gtgcacagcc agttcctacc actttggtca ttaacggatc gccaccgtga atgccaatga
     7381 tacgatgtcc atcggtaaca taatccacct cgccagtgac cacgacgatt gcgccagttt
     7441 cccgtgccag tgtttgtgca gcgggtatcg cgttagctgc ggcatcagtg gtatccactc
     7501 cccgtccacc attagcaacg ccagctaatg ccatgatttc cgaagcatta ccacgtatgg
     7561 cggttggctt atgggacaga agttccacac aaaaacggcg gcgataatcg agcgcaccaa
     7621 ccgcaaccgg atcaagcgtc cagggcgttt gtgatcgtgt tgcttgctca acggcagcac
     7681 tcatcgcttg cgctcgtggc tgcgtcagcg tgccaacgtt aatcaacaag gcactggcga
     7741 tagccgcaaa ctgactggcc tcttcggttt cgataaccat cgctggcgat gcgccgagcg
     7801 ccagcaaggt attggcagta aaagtttgca ccacatcgtt ggtcatgcag tgcacaagag
     7861 gggaatgttg gtgaaaaagg tgtaacgcgt gcgcagattg cgctgaactc agcaggtcga
     7921 cttgcatagt ttgctcctgc cataacgtga agaagcaatg acctggtggt ccgtgacttc
     7981 cctacgctgg cattatccag atcaggtgat acgggtattt ctcagccttc acaaagaagg
     8041 gcaccccgag tcgtttggtt gcgataataa atcgcagagg aggatggtaa tgtccagcgc
     8101 acgcgttgta aacggggaaa aacggcggta ttacaataac gttgtgaaat tatttgatat
     8161 aagaatccag caccttcaga acgacatcca gatcttcttc acgttttagc tcatccccct
     8221 ggtgaacgat gtgttccgtc agatggcctt taatcacttc ccgcatcaga ccgtttaccg
     8281 cgccacggat agcagcaatc tgttgtaaaa ctgcagcgca ttcatgcggt tcgtcgagca
     8341 ttttcttgag cgccacgacc tggccctgaa tcttactggc acgcgctttc agtttctgtt
     8401 tatcacggat tgtatgagac atggcaacac ctggttaaca agaatatgaa aaatcatagc
     8461 actattaatc tactgggggg tagtatcagg tactgggggg gagtagaatc agattgccga
     8521 attaatacta agaattatta tcatgaccga atttacaaca cttcttcagc aaggaaacgc
     8581 ctggttcttc atccccagcg ccatcttact tggtgcgctt catggcctgg aaccggggca
     8641 ctcaaaaacg atgatggcgg cgtttatcat cgccatcaaa ggcaccatta aacaggcggt
     8701 gatgctcgga ctggcagcaa ctatttcgca taccgcagtg gtctggttaa ttgcctttgg
     8761 cgggatggtg atcagcaaac gctttactgc tcaatcagca gaaccgtggc tccagttgat
     8821 ttccgcagtg atcattattg gcaccgcgtt ctggatgttc tggcgtacct ggcgcggcga
     8881 acgcaactgg ctggagaata tgcacgagca tgatcatgag catcatcacc acgatcacga
     8941 agatcaccac gaccatggac atcatcacca tcacgaacat ggcgagtatc aggatgccca
     9001 tgcacgagcc catgccaatg acattaaacg acgctttgat ggtagagagg tcaccaactg
     9061 gcaaattttg ctatttggct taaccggtgg ccttatcccc tgcccggcag caattaccgt
     9121 gctgttgatt tgcattcagt tgaaagccct gacactgggc gcaacactgg tcgtcagttt
     9181 cagcctcggc ctggcgttaa cgcttgtcac cgtaggcgtt ggcgcagcaa tcagcgttca
     9241 gcaggtcgca aaacgctgga gcggatttaa cactctcgct aaacgcgctc cctatttttc
     9301 cagtctgttg attggcttag ttggtgtgta tatgggcgta catggcttca tgggcataat
     9361 gcgataactc aatcctttag gcttgcatgt tatcttcagc ccaggatgat tcttaaatca
     9421 gctatttccg ctgactctct gctcgctaag gatgctttta gggcatcctt tcatttacac
     9481 tttttacgaa atcatgggat cactaacaaa atatcgcttg tcagttatat tgtatggcag
     9541 gaaagatatg cgactgatat tacagatccc caaagtggag agtttatgac cattaaaaat
     9601 aagatgttgc tgggtgcgct tttgctggtt accagtgccg cctgggccgc accagccacc
     9661 gcgggttcga ccaatacctc gggaatttct aagtatgagt taagcagttt cattgctgac
     9721 tttaagcatt tcaaaccagg tgacaccgtc ccagagatgt accgcaccga tgagtacaac
     9781 attaagcagt ggcagttacg taacctgccc gcgcctgatg ccgggacgca ctggacctat
     9841 atgggtggcg cgtacgtgtt gatcagcgac accgacggta aaatcattaa agcctacgac
     9901 ggtgagattt tttatcatcg ctaaaaaaaa gccccctcgg agagaggggg gaatgcagac
     9961 accttaatat ttttattatt agccacttgc tcgtcttgct tgttattagt cgtatttcac
    10021 gttgattaat gcggttgcct ccagtgcgcc agatttaact ttgtttgtat cgtagacgta
    10081 gtaactggcc gttatcggaa ttgaaaccgt tgtatctgta agttttgact ttgccgcaat
    10141 ttcttttaaa tttgtatatc ctttactgct gttagtgata tttaaaagtg tgactggcgt
    10201 accattactt tcaagaataa atcctacgcc attatcttcc gcacctttca ggatcgtgtt
    10261 gctgttggtt aacaagtcag aacttgataa atacacctgc attttccgtg atgtttgatt
    10321 ttgttgacct aataaatttt tacaacgcag aataatatcc ccttttttac tatttattgt
    10381 tgttttgtta cctgtggctg gtaattgtga aacgggaata ctctctaacg taattaataa
    10441 atcgtccggt ttgcaggttg tggttatcgg gttgtatgac aatgtcagat taaaggaata
    10501 gacacctgca ttttgtgcca atgcatttct gtagctattt acacatgcat cccatgactt
    10561 accacctaat aaacacagta gaaattgtac tgcggcactc ccgcgtgtcg atgatgaagc
    10621 actggataat gtcgcaactc cattaatcat cactgaacca ccgccccccg cagaaacatt
    10681 cacttttgca ttactggccg caggtgtcac cgtcaccgaa taaccactgg tgaaactggt
    10741 ctgattattt gacaacgtaa tgttgttttt attggtccat tcaaaatgta aatttaattt
    10801 aatggtatcg ttataaagtc caacaataat atccgacggg ctcataggtg tgatagtatt
    10861 tgtactcacg catgacgaat cattaaaatt atgctcatat gtcgccattt gtgtttgagt
    10921 aagtgcaaaa tccccctgaa caaccaaatt gtgctgaaca gataactcac tataacatgc
    10981 cgcatagctc acaggcataa ccatcatcat tactataaat agcattatgc gaatttccat
    11041 agaaccacct gactattact ggcaaatata atttctactt tcatcaatct ctttaccgaa
    11101 ggtgattgtg catgaaagcc cttgttgctt atcaattgcc acattaaccg atggcggtac
    11161 ttcattggtg cgaataaata actggcttcc ctggccgaca acaccaatat tatgaccatg
    11221 gatatcattg acttcataac caaacgttaa tggttgccca tccgctctta acgcttttat
    11281 aaaccatggc ttgcgctgat cggtatcaaa attaaccagt acaaccgcgc cgcgataagg
    11341 ggcggcaatt ttccggttgc cacgtaattc tgcttcgcta tcgctttgtg acacatccaa
    11401 catcaggtaa ttttcccgat aaggtgtcat tccgtcgtat accaccactc cattacggtt
    11461 tgttgtgcga tatttttgcc cattgacata agcatcttta attcctgccg cattcatcac
    11521 agcaaacgtt tcagaaagac ggtttgccag attaacgcca cccgaccagg cgacaatgcc
    11581 ccctgaaaca ctggctccag cctgtcgata agcactcgac tgactataac tgccattcac
    11641 tgtcgcaacc ggcgcgttcc aggttaaatt cgccccagct gtcgtttcat ttccctgata
    11701 ctgataactc aggttgaccc catagttaaa ctgatcccgg cttccaacgg ttcctgataa
    11761 tcccgtatta tttgaggcaa ccccctgatc atcaaacgtc gttgagttag acatatatat
    11821 ttgccgacga ggcgtcgtaa cgtcatcacc ccaatcaaag ggaatcgata taaaaatatt
    11881 aaaacgtttc tcttcatgat gattctcgtc ataagcctgg cttgccgcga gggtatagct
    11941 tatccttcgc aggttgttgg aataactcaa ctgataatcc ttactgctgc cgctacgccc
    12001 ccagtaatct cgccataacg tacttaagga cacagaaccc cagccttctg gcaatgactg
    12061 actcatattg gcagaaaagc tatttttacg gccaaaatcg ttctggtaat aatcggcaat
    12121 gtcatagaca tcgttttcat cacggcgata attatcttta ttgtttgccc aaacgtgatc
    12181 gttaaatgtc cggtaatcac gcgacgaata acgccaggcc gccagaccaa aacgtgtcga
    12241 cgtttggctc acaaatttgt tgtaggcaat ttgataactt tgcccgtcaa acacatcgcc
    12301 gttgtcttgt ttactatgcg acttcgtggc atcgacggaa atggcaccaa tgcgtgtatt
    12361 ccagccagtc cccaaagtga acgcgtaata attattcgca accatcgagc caccatacag
    12421 cttcaataag ttattaaaac catactgata acccgccagg acaaaatcac tttgtttgct
    12481 cgccccttca atatggctac gacccgccgc aaaatcatat ttcgacacgc caggttgcag
    12541 catattcggc accgctgcat aaggcaccag ataggtggtt accgaaccgt ccgcctcttt
    12601 cacgctgaca tcaagatctg ctccaccacc ggccaactgc aaatctgtaa tcgcaaacgg
    12661 gccaggagga acctctttct gataaaccac aaaaccgttc tgttcaatag ttaccagcgc
    12721 gttactctga gcaatcccct gcacccgtgg cgtaaaattc tgtttcgagt taggcaacat
    12781 ctgcatatca cgaaacaacc gcacaccgct gaagcgaaca gaatcaaaaa tatcgcttga
    12841 tgtgtacata tcacccacgc gaagcgtgcc gagaagttgg gcaaatccac gttccagata
    12901 cagggtattg cttttccaca cccctggatt gctatttgtt ttactgaaac tggcatcaga
    12961 atgcagttgc caccccagta agtttaaccc gctgttaaaa cgtacatatg tactcttgct
    13021 attacccgac gctttatagt cgctgtaata ctgactcaca taataagagg tataaaacgc
    13081 attaataccc cgctcccagt tttccggtgg aacatagcca ctttccagtt cttccaccca
    13141 cgcctgcggg acactgaaat cgagacgaaa aactccgata tcccaggtat agctcccacc
    13201 ctgaacaagt tgctcaaatg ttaaacattg cttaccactg gcaaagttat cggtattaat
    13261 gcctaaccgc ttgataattt cccttgataa acaggtttct tgcgggttgt ctttaacaat
    13321 aatctcatat ttcccgcgcc attgcttatt gacataaata tcgatgtcat actgccccgg
    13381 caagggttga ttatcatcaa gacgaatatt tgcaacctgc tggtctttca ttccacctat
    13441 catgaaatgg gtatcaaagg tttcttcagc tgcataagct tcaatgccga gcaataacgc
    13501 tacgattgct gatgcaagtg gggtcattct caacataggc agctcctgca attaaatttt
    13561 gtcactaata tagttgccat ggtcatcgat aatggtcaga tgccagttat tcgcattatt
    13621 acttttgaca ttaatactct gactttctaa gggggcaatc ataatagttt cataattgac
    13681 tttgacatta ttagctttga catccgaaat cgtcacccaa tttgctgagt cattttttat
    13741 caccaatcca ttgccactgc gatttaccag taacttttta aatgtcgctt tatttaccgg
    13801 tgcaataccc gcaggccggt aaaacaattt gattttgttt tgcatcgcaa acttcagtgc
    13861 attcttacct tcttgttctg gactatttgg tggaatatcc agaacattca gataaaaaat
    13921 gctttcttta ttagtgggca gtttattcgg cataattttg atttttactt gctgcccgga
    13981 atttgcccct atttttgcca ctggtggcgt taacatgaaa ggaagctgaa ttttttctgg
    14041 tggtaacgac gtatcaccat catcaatcca cgcctgcagc agcgaagaac ggtttccctg
    14101 attcatcaac tgcaccatca cttctttatt ttctgccggg taaataatac gcgtcccgta
    14161 gataacaata ccggcatgtg caggaaggac catagaagaa atgagtaaag ctaataatcc
    14221 tttcatctta atgcctctta aattaaaagg ccgccatgga gatagcggcc atgttaatta
    14281 cctgaagaat cttgcaattt tatttattaa tcctgcgcca cacgcataat cagggaagat
    14341 aacacatcac ctgaattcac tgagttatct gtcagtttca ggtattttgc tttaaacgga
    14401 atggcatatt gaccgtccgc tgttttataa ctctcgatgt tgaaaggtgc agaaacatcc
    14461 aggacatcgc tgttatcacc taaatagaga taataaccca cggtatcgtt gctactggct
    14521 atcgccttat cgtttccggt tattttatcg ttggtaatca acgagagttt tacaccagca
    14581 acttctttcg gacagctaat ttttaacatg ctgctggtta caccgactgc accatcatag
    14641 gtcgagtatt tagctttacc ttcaaagacc gtagttggta cagttggcat attaatactt
    14701 actgaatcgc cacctgcttc gaatttacac ggagattctg caatattccc tttaatggtt
    14761 aatgttccgg tatcaacgtc tgcggcaaat accccagcgc tcataaaaaa agaagaaaaa
    14821 acagcggcag caataattga acgtttcata atatgctcct taataaaatc tatttttggt
    14881 tgttaagagt ctgtatctct tggaaacaaa tttctcacac caaaaaagtt gccgcaatgt
    14941 aaacacattg ttttattgat ttaaagcaaa ttaactattg caatataaac acaacaaata
    15001 cttaagcaca ttaaa
//
LOCUS       DAZKHK010000118        14898 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKHK010000118 DAZKHK010000000
VERSION     DAZKHK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.118"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(306..1955)
                     /gene="pgi"
                     /locus_tag="ACHOS5_003886"
     CDS             complement(306..1955)
                     /gene="pgi"
                     /locus_tag="ACHOS5_003886"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9778629.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2192..2388
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2480..3829
                     /gene="lysC"
                     /locus_tag="ACHOS5_003887"
     CDS             2480..3829
                     /gene="lysC"
                     /locus_tag="ACHOS5_003887"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP9778630.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            4082..4354
                     /locus_tag="ACHOS5_003888"
     CDS             4082..4354
                     /locus_tag="ACHOS5_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP9778631.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4355..5227)
                     /gene="rluF"
                     /locus_tag="ACHOS5_003889"
     CDS             complement(4355..5227)
                     /gene="rluF"
                     /locus_tag="ACHOS5_003889"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP9778632.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5575..6522
                     /locus_tag="ACHOS5_003890"
     CDS             5575..6522
                     /locus_tag="ACHOS5_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9778633.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFSLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6592..7398
                     /locus_tag="ACHOS5_003891"
     CDS             6592..7398
                     /locus_tag="ACHOS5_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9778634.1"
                     /translation="MQTWLNLQDKIIIVTGSASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7408..7815
                     /locus_tag="ACHOS5_003892"
     CDS             7408..7815
                     /locus_tag="ACHOS5_003892"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP9778635.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7815..8309
                     /locus_tag="ACHOS5_003893"
     CDS             7815..8309
                     /locus_tag="ACHOS5_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9778636.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8375..9172
                     /locus_tag="ACHOS5_003894"
     CDS             8375..9172
                     /locus_tag="ACHOS5_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP9778637.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            9183..10007
                     /locus_tag="ACHOS5_003895"
     CDS             9183..10007
                     /locus_tag="ACHOS5_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP9778638.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            10059..11288
                     /gene="sorE"
                     /locus_tag="ACHOS5_003896"
     CDS             10059..11288
                     /gene="sorE"
                     /locus_tag="ACHOS5_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP9778639.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLVLGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11669..12358
                     /gene="pepE"
                     /locus_tag="ACHOS5_003897"
     CDS             11669..12358
                     /gene="pepE"
                     /locus_tag="ACHOS5_003897"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP9778640.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLASLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGEPREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12448..14079)
                     /locus_tag="ACHOS5_003898"
     CDS             complement(12448..14079)
                     /locus_tag="ACHOS5_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP9778641.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14250..14699)
                     /locus_tag="ACHOS5_003899"
     CDS             complement(14250..14699)
                     /locus_tag="ACHOS5_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778642.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNGRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQIVLLFSLFFSAQCFLRQKLFSFSFLAIQEFMRFAT
                     VSCSISVIPLLLNYFHSQNLVLNISLFIFSEVTKVTTIIWCKRQGKV"
BASE COUNT         3710 a         3721 c         3817 g         3650 t
ORIGIN      
        1 ataaaaagta attactctgc gtattttccg aaataaacca cgattaaaaa tttattttag
       61 gattaacctc acggtatgat tttcgttaaa tgacagacaa gaggatttat ctgataaaac
      121 aatgccgact tatagtcggc attgttttta aggctattta atcaatcgta ctatgtatta
      181 attgccgaat acgatgtaca tatcggcatc gacctgtagg cctgataaga cgcgaccgcg
      241 tcgcatcagg catcggttgc cggatgcggc gtgaacgcct tatccggcct acatatcgac
      301 aatgattaac cgcgccacgc tttatagcgg ttaatcagac cattggtcga gctatcgtgg
      361 ctgctgattt ctttgtcatc tttcagctct ggcagaatac ggttcgccag ctgtttaccc
      421 agttccacgc cccactggtc gaaggtgaag atgttcagga tcacgccctg agtaaagatt
      481 ttgtgctcat acagcgcaat caacgcaccc aggctgaacg gagtgatttc acgcagcagg
      541 atggagttag tcgggcggtt accttcgaac actttgaacg gcaccacgta gtcaagcgtt
      601 gccggatctt taccctgatc gcgatattcc tgctcaacca cttcgcggga tttaccaaac
      661 gccagcgctt cggtctgagc aaagaagtta gacagcagtt tctggtgatg atcagagagc
      721 gggttatggg tgatagccgg agcgatgaaa tcgcacggta ccattttggt tccctggtgg
      781 atcagctgat agaacgcgtg ctgaccgttc gtgccaggtt caccccagat aatcgggcca
      841 gtctggtaat ccacaacctt accgttacgg tcaacatact taccgttgga ctccatattg
      901 ccctgctgga agtacgccgc aaaacggtgc atatactggt catacggcag aatcgcttca
      961 gtttccgcgc caaagaaatt gttgtaccag atgccaatca gcgccagcag tacaggcagg
     1021 tttttctcgg caggcgtggt ggagaaatgc ttgtccatcg cgtgtgcgcc agaaagcagt
     1081 tcaacgaagt tatcaaagcc gatggagaga acaatcgaca ggccaatcgc tgaccacaaa
     1141 gagtaacgac cgccgaccca gtcccagaac tcgaacatgt tggcagtatc aataccaaac
     1201 tcgccaacgg ctttggcgtt ggttgagagc gccgcaaagt gtttcgcaac gtgcttctca
     1261 tcacctgccg ctttcaggaa ccagtcacgc gcgctgtggg cgttggtcat ggtttcctga
     1321 gtggtgaagg ttttagatgc taccaggaac agcgtggttt ccgggtttac ttttttcagc
     1381 acttccgcga tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag gtggtttttg
     1441 tatggacgca gtgcttcagt caccatgtac gggccgaggt cagaaccgcc gatcccgatg
     1501 ttcactacgt cagtgattgc cttgccggta tagcctttcc actcaccgga aataatcgct
     1561 tccgagaagg ttttcatctt ctccagcacc gcgttgactt ccggcataac atctttgcca
     1621 tcaaccagaa tcggggtatt gctacggtta cgcagcgcta cgtgcagtac ggcgcggttt
     1681 tcagtgcggt tgatcttctc gccagagaac atcgacttaa tcgcgcccgc cagatcgcac
     1741 tctttcgcca gatcttgtaa tttcgccagc gtctcttcag tgatgcggtt tttggagtaa
     1801 tccaccagca tctgatcgtc gaaggttgcg gagaacttag aaaaacgatc gccgtcttta
     1861 gcaaaaagat cggcgatcgt aacgtctttc atttcatcga agtgtttctg tagtgcctgc
     1921 caggcagcgg tctgcgttgg attgatgttt ttcattagca atactcttct gattttgaga
     1981 attgtgactt tggaagattg tagcgccagt cacagaaaaa tgtgatggtt ttagtgccgt
     2041 tagcgtaatg ttgagtgtaa acccttagcg cagcgaagca tttattagct gaactactga
     2101 ccgccaggag tggatgaaaa atccgcatga ccccatcgtt gacaaccgcc ccgctcaccc
     2161 tttatttata aatgtactac ctgcgctagc gcaggccaga agaggcgcgt tgcccaagta
     2221 acggtgttgg aggagccagt cctgtgataa cacctgaggg ggtgcatcgc cgaggtgatt
     2281 gaacggctgg ctacgttcat catcggctac aggggctgaa tcccctgggt tgtcaccaga
     2341 agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa atagtagcga
     2401 agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct gaaaatggat
     2461 cccctgacac gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg gtaccagcgt
     2521 agctgatttt gacgccatga accgcagcgc tgatattgtg ctttctgatg ccaacgtacg
     2581 tttagttgtc ctctcggctt ctgctggtat cactaatcta ctggtcgctt tagctgaagg
     2641 actggaacct ggcgagcgat tcgaaaaact cgacgcaatc cgcaatatcc agtttgccat
     2701 tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc tgctggagaa
     2761 tattactgtt ctggcagaag cggcggcgct ggcaacgtct ccggccctga cagatgaact
     2821 ggtcagccac ggcgagctga tgtcgaccct gctgtttgtc gagatcctgc gcgaacgcga
     2881 tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt accaacgatc gatttggtcg
     2941 tgcagagcca gatgtagccg cgctggcgga actggccgcg ctgcagctgc ttccacgcct
     3001 caatgaaggc ttagtgatca cccagggatt tatcggcagc gaaaataaag gtcgtacaac
     3061 gacgcttggc cgtggaggca gcgattatac ggcagccttg ctggcggagg ctttacacgc
     3121 atctcgtgtt gatatctgga ccgacgtccc gggcatctac accaccgatc cacgcgtggt
     3181 ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga tggcaacttt
     3241 tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca gtacgcagcg atatcccagt
     3301 ctttgtcggc tccagcaaag acccacgcgc aggtggtacg ctggtgtgca ataaaactga
     3361 aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc tcactttgca
     3421 cagcctgaat atgctgcatt ctcgcggttt cctcgcggaa gttttcggca tcctcgcgcg
     3481 gcataatatt tcggtagact taatcaccac gtcagaagtg agtgtggcat taacccttga
     3541 taccaccggt tcaacctcca ctggcgatac gttgctgacg caatccctgc tgatggagct
     3601 ttccgcactg tgccgggtgg aagtggaaga gggtctggcg ctggtcgcgt tgattggcaa
     3661 tgacctgtca aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg aaccgttcaa
     3721 cattcgcatg atttgttacg gcgcatccag ccataacctg tgcttcctgg tgcccggcga
     3781 agatgccgag caggtagtgc agaagctgca ttttaattta tttgagtaaa cacagtatgg
     3841 cccggaagac attatttcgg gccgtattgt ttttcttgtc attatgccca taaataaacg
     3901 agtctattcc ctgttaacca gcgtctttat cggagaataa ttgcctttaa ttttttatcc
     3961 gcatctctaa ctaattatcg aaagagataa atagttaaga gaaggcaaaa tgaatattat
     4021 cagttctgct cgcaaacgaa ttcccgtaca ctgaactctc tggctcactg agggcaccat
     4081 catggcactc ccccgtatta cccaaaaaga gatgactgaa cgcgagcagc gtgaacttaa
     4141 aacactgctg gatcgggcgc ggattgcgca tggtcgcgta ctgaccaact ccgaaactaa
     4201 cagcattaag aaagagtaca tcgataaatt aatggtcgag cgtgaggctg aagcgaaaaa
     4261 agcccgccaa ttgaagaaaa agcaggctta taaaccggat cctgaggcgt catttagctg
     4321 gtcagcaaat acgtcgacgc gcggcagacg ctgattaacg ccccttcttt ttgcgccccg
     4381 gcgaggtaaa acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc gttttttcca
     4441 tcttaacgac tggacgtttg atgcctaccg ttttcggttt ggccttcgct ttaggttttg
     4501 cctcggaaga ggaattttca atgagcttaa agagatcgat taactcatcg tcggttaaat
     4561 cgcgccactc acccagcgga atgccgctta agctgacgtt cataatgcgc gtacgttcca
     4621 gctttttcac ttcgtaacca aagtgctcgc acatgcgacg aatctgacgg ttcagcccct
     4681 gcaccaaggt aatgcggaag acaaacggtg cttctttttt gactttgcac tttttagtta
     4741 ccgtcccgag gatcggtact cccgccccca taccacgaat aaattcatcg gtaatcggtt
     4801 tatcaaccgt caccagatac tctttctcat gatcgttacc cgcacggagg atcttattca
     4861 ccagatcgcc gtgattggtg aggaaaatca gcccctggga atctttatcc aggcggccaa
     4921 tcgggaacac gcgtttgctg tggttaacga aatcgacaat gttatcgcgc tcgccatctt
     4981 cggtggtgct gacaataccg acgggcttgt tcagggcgat aagtaccaaa tcttcggatt
     5041 cccgaggctc aatcaactga ccatttactt tcacaacgtc gccgggtttc acctgatcgc
     5101 caatggtggc tcgcttgcca ttaaggaaca cattgccttg ctcgatatag cgatccgctt
     5161 cgcggcgtga gcaaattccg ctttcgctga tgtatttatt taaacggact gatgagtcgg
     5221 gcagcataaa ttctcctgta aaacgcggaa tataccgtac ctcttagcgg ggaaaaacag
     5281 ggttatgcgg caacgtcgaa attaaatgtg atctgccacg ccttttatca caaaagtgtc
     5341 tccggaagta cgatgcaaat ttgactggca aaataagaat aatcaacaaa ttacgaatcg
     5401 ctgcacacct gtgctgatga ctactacgca gagaaacaaa tgtgcaagag aaagcgctat
     5461 ttccccccat gcaaagagtg aaaaatcgct ttgaaaggcc atcatgatgg ggttatatca
     5521 gatcaagcgg tgagaaagcg tatttgcaca aatgtgcaga accaggaggc ggcaatggaa
     5581 aacagtgacg atatccgttt gattgtgaag attgcccaac tctattacga acaggatatg
     5641 acgcaggcgc aaattgcgcg cgaactgggt atttatcgca ctaccatcag ccgcttgctt
     5701 aaacgaggcc gcgatcaggg aattgtcacc atcgccatca actatgacta caacgaaaat
     5761 ctctggctgg agcagcaagt gaagcaaaag tttagcctga aagacgttgt ggtggtgtca
     5821 ggaaatgatg aggatgaaga tactcaactg gcgatgatgg ggttacacgg cgcgcaactg
     5881 ctggatcgct tgctggaacc tggcgatatt gtcggttttt cctggggccg cgcggtgagc
     5941 gcactggttg aaaacttgcc gcaggcgggg caatcgcggc agttaatttg cgtgccgatt
     6001 attggcggcc cgtccggtaa actcgaaagc cgctatcacg taaacacatt aacctacagc
     6061 gcggcagcga agctgaaagg ggaatcgcat ctcgcggatt ttccggctct gctggataac
     6121 ccattaattc gtaatgggat catgcagtct cagcacttta aaaccatctc tgcctactgg
     6181 gataatctgg atgtcgccct ggtgggaatt ggctcaccgg ccattcgcga cggcgctaac
     6241 tggcatgcgt tttatggtgg tgaagagagt gacgacctga atgcccgcca ggttgctggc
     6301 gatatttgct cgcgcttttt tgatattcac ggcgcaatgg ttgaaacgaa tatgagcgaa
     6361 aaaacactct ctatcgaaat gaataaatta aagcaggcac ggtattccat tggcattgcc
     6421 atgagcgaag aaaaatacag cggaattgtt ggtgcactgc gtggaaaata tattaattgt
     6481 ctggtaacga atagcagcac agctgaacta ttactgaaat aaaaatcttc ccgatgttta
     6541 ccgtaataaa aattacggcg ggtccctatt atctaaaatc aggagtctgt tatgcaaacg
     6601 tggttaaatt tgcaggataa aatcattatt gtcaccggta gcgcatccgg cattggtctg
     6661 gcgattgtgg atgaattatt agcacaaggc gcgaatgtac agatggtcga tattcacggt
     6721 ggcgatggtc agtatgaaag ccataaaggt tatcagttct ggccgaccga tatttccagc
     6781 gccaaagaga taaatcatac ggtagcagaa attatccagc gttttggtcg catcgacggt
     6841 ctggttaaca acgccggggt caatttcccg cgtctgcttg tcgatgagaa agcgcctgcc
     6901 gggcagtatg aactcaacga agctgcattc gaaaaaatgg tcaatatcaa ccagaaaggc
     6961 gtttttctga tgtcgcaggc ggtggcgcga cagatggtca aacaacatga tggcgtgatt
     7021 gtgaatgttt cctcagaaag tgggctggaa ggctcagaag gccaaagctg ttacgccgcg
     7081 accaaagccg cgctcaatag cttcacgcgc tcctggtcga aagagctggg caagcacggt
     7141 atccgtgtgg tcggtatcgc gccggggatt ctggaaaaaa caggactgcg cacgccggaa
     7201 tatgaagaag cgctggcgtg gacgcgcaat atcaccgtcg agcagctgcg tgaaggctat
     7261 accaaaaacg ccattcctat tgggcgcgcc ggaagattag cagaagtggc tgattttgtt
     7321 tgttatctgc tgtctgaacg cgccagctat atcaccggag taaccactaa cattgcaggc
     7381 ggcaaaacgc gcggctaagg aggctttatg gtcaatgcca ttttttgcgc ccacggcaaa
     7441 ttagcctgcg ccatgctgga atcggttcag atggtgtacg gcgatgccag ggtcgaggcc
     7501 gttgaatttg tgcccggcga gaacgccgga gacattgtgg caaagctgga gaagttagta
     7561 agcattcaca atcaagatga gtggctaatc gccgtggatt tacagtgcgg cagcccgtgg
     7621 aatgcggcag cgatgcttgc gatgcgcaat cctcgtctgc gggtaatcag cggcctttct
     7681 ctgccgctgg cgctcgaact ggttgataac caggacagca tgaatattga tgaactgtgt
     7741 gaacacctga cgcaaatcgc aaagcaaacc tgcgtcgtct ggagacaggt ggcaacagcg
     7801 gaggaggatt tctgatgaac attacgctcg cccgcattga tgaccgcctg atccacggcc
     7861 aggtcactac cgtctggtct aaagtggcta acgcccaacg cattattatc tgcaatgacg
     7921 aagtttataa cgatgaagtt cgccggacat tattgcgtca ggctgcgcca ccgggaatga
     7981 aggttaacgt ggtcaatatt gaaaaagccg tcgcggttta tcacaacccg caatatcagg
     8041 acgaaaccgt tttttatctt ttcactcgcc cacaggatgc cttagcaatg gttcgccagg
     8101 gggtaaaaat aggtaccctg aatattggcg gtatggcctg gcgtcctggt aagaaacagt
     8161 taaccaaagc agtttcatta gatgacgatg atattaacgc atttcatgag ttaaataatc
     8221 ttggcgtcat actggattta cgcgtcgtag cgtcagatcc ctcaatcaat attatcgaca
     8281 aaataaacga acagttaatc gcaaattaaa taatagcgcc tgtgtacata tgctttaaca
     8341 ggcagggatg acgacactta aaaggtgaca aatcatggaa ataagtaccc tacaaatcat
     8401 tgctatattt cttttttcct gtattgccgg aatgggcagc gtgctggatg aatttcagac
     8461 tcatcgtccg ttaattgcct gtacggtgat tggtttaatt ctcggtgatt taaaaaccgg
     8521 aattatgctc ggtggtacgc tggaattgat agctctcggc tggatgaacg tcggcgcggc
     8581 gcaatctccg gattctgcac tcgccagcat aatctccgcc attctggtta tcgttggtca
     8641 gcaaagcatc gccaccggaa tcgccatcgc gttgcctgtg gctgcggcag gccaggtgct
     8701 gacggtgttt gcccgtacca tcaccgtagt gttccagcac gcggcggata aagcagcgga
     8761 agaagcacgg tttcgcactc tcgatattct gcatgtctcc gcgcttggcg tgcaggcact
     8821 gcgcgttgct attccggcac tgattgtctc gctgttcgtc agcgcagata tggtgagcaa
     8881 tatgctgagc gccattcccg aatttgtgac ccgtggactg cagattgctg gcggttttat
     8941 cgtggtggtc ggttacgcca tggtgcttcg catgatgggc gtgaaatatt tgatgccttt
     9001 ctttttcctc ggtttcctcg caggtggcta cctcgatctc agtctgctgg cgttcggtgg
     9061 cgtcggcgtg atcatggccc tgctctacat ccagttaaat ccacagtggc gtaaagctga
     9121 accacatccc cagaccacca ctatcaccgc ccttgaccaa cttgatgatt aacggagcca
     9181 tcatggaaca gagaaaaatt acacgcagcg atctggtgag catgtttctg cgctccaacc
     9241 tgcaacaggc atcctttaac tttgaacgta ttcacgggct gggtttttgc tacgacatga
     9301 tccccgccat caaacgactt tacccattaa aagaggatca ggttgcggcg ctcaggcgac
     9361 acctggtgtt cttcaatacc acgccagccg tatgtggccc ggtcatcggc gtcactgccg
     9421 ccatggaaga ggcgcgagcc aacggcgcgg aaattgatga cggtaccatt aacggcatca
     9481 aagtcggtct gatgggacca ttggcaggag ttggcgatcc actggtctgg ggaacgctgc
     9541 gcccgattac cgccgcgctc ggcgcatctc tggcactttc gggcaacatt ctcggccccc
     9601 tgctgttctt ctttattttc aacgcggtgc gtctggcgat gaagtggtat ggcctacagc
     9661 tcggctttcg caaaggggtg aatatcgtca gcgatatggg cggtaatttg ctgcaaaaac
     9721 tcaccgaagg cgcgtcgatt ctcgggctgt ttgtgatggg cgtgctggtg accaaatgga
     9781 cgtcaatcaa cgtaccgttg gtggtatcac aaacgcctgc cgccgatggt gccaccgtca
     9841 ccataaccgt gcagaacatc ctcgaccaac tttgccctgg tttgctggcg ctcggtctga
     9901 cgctgctgat ggttcgtctg ctcaacaaaa aaattaaccc ggtatggctg attttcgccc
     9961 tgtttggttt agggattatc ggtaatgcgc tgggcttcct gtcctgattc ttcgccccgg
    10021 cgcaaccgtc ggggccatcg ttcaacatga ggtggtttat gaaaacaaca gctctgcgtc
    10081 tttatggcaa acgtgacctg cgtctggaaa cctttgacct tcctgaaatg caggaggatg
    10141 aaatcctcgc aacggtggtc actgacagcc tgtgcctctc ttcctggaaa gaggccaatc
    10201 tgggtgaaaa ccataaaaaa gtacccgacg atgtggcgac caatcccatc atcatcggcc
    10261 acgaattttg cggcgatatt ctggccgtgg gtaaaaagtg gcagcacaaa ttccagccgg
    10321 gtcagcgtta tgtgattcag gccaacctgc aactccccga ccgcccggat tgccccggct
    10381 actccttccc gtgggtaggc ggcgaagcga cgcatgtggt tattcccaac gaggtcatgg
    10441 aacaagattg cctgctggca tacgacggcg aaacctattt tgaaggctcg ctggttgaac
    10501 cgctttcctg cgtgattggc gcgttcaacg ccaactatca tcttcaggaa ggtagttata
    10561 accacacgat ggggattcgc ccgcaagggc gcacgctggt cctcggcggc accggcccga
    10621 tggggctgct ggcgattgat tatgcgctac atggacccgt taacccttcg ctgctcgtca
    10681 ttaccgatac cgacaacgac aaattgagtt atgcgcgcaa gcactatcca tcagaaccgc
    10741 aaacactgat tcattatctc aatgccaccg atgcagcatt tgatacgtta atggcactga
    10801 gtggcggtca cggcttcgat gatattttcg tctttgtgcc taatgaagga ctggtgactc
    10861 tcgcctcttc cttgctggcg acagatggtt gtctgaattt cttcgccgga ccgcaggata
    10921 agcacttcag cgcgccaatt aatttctacg atgtgcatta tgcatttacc cactacgtgg
    10981 gcacatcagg cggcaatacc gacgacatgc gcgcagcggt caaattgatt gaagagaaaa
    11041 aagtgcaggc cgcaaaagtg gtaacacata ttcttgggct gaatgccgcg ggcgaaacca
    11101 cgcttgaatt gcctgccatc ggcggcggca aaaagctggt gtataccggg aaatacctgc
    11161 cgctgacgtc actcacgcag attcaggatc aagcactggc ggcaattctg gcgcgtcatc
    11221 aggggatctg gtccggtgag gcggagcaat acctgctcgc ccatgcagag gcaatttccc
    11281 atgattaatc gcgctactta actctgcatg tcgctggctg gtcgccgcgg caacttcgcg
    11341 acctgatgtg cggctgattg ctgaagcggc acaatgtgca cgagaagaat aaaaaaacat
    11401 cagcgcctta aggggcgctc aaattggtgg caaagtgcac attgtttatg ccggatgcgg
    11461 aatgaagcct tattcgccta cataatcttg aaaattcaat atattgcagt tatctggtag
    11521 gccaggtaag cgtaacgcat caggcaatcc tgtgtttgtc atcagtctca gcgcctgtgc
    11581 aatatgtgac atcgtgagcc atgtcagtaa gtggttaaat tcgcagtggt agcattcctt
    11641 ccgtgttttc ttactaaccg gagaagtaat ggaactgctt ttattgagta actcgacgct
    11701 gccgggaaaa gcctggctgg aacacgcgct gccgctgatt gctgaacagt tgcagggtcg
    11761 ccgttctgcg gtatttattc ctttcgctgg cgtaacgcag acctgggatg attacacagc
    11821 gaaaacggct gcggttctcg cttcgctggg tgtttctgtc accggtattc atagcgttgt
    11881 cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt gtcggcggcg gtaatacttt
    11941 ccagttgctg aaacagtgcc gcgagcgcgg gctgctggca ccaattactg acgtggtgaa
    12001 acgtggcgcg ctgtatattg gctggagcgc aggcgctaac cttgcttgcc caaccattcg
    12061 taccaccaac gatatgccga ttgtcgatcc gcaaggtttc gatgcgctaa atctgttccc
    12121 gctgcaaatc aacccgcact tcaccaacgc gctgccggaa ggccataaag gtgaaccccg
    12181 tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa ctgacgatta ttggtctgcc
    12241 ggaaggtaac tggatcacag taagtaaagg tcacgctacg ctgggtggcc cgaacaccac
    12301 ttatgtgttt aaggctggtg aagaagcggt tccgctggaa gcgggtcacc gtttttaagt
    12361 gaattgccgg atgcatgatc cggcatcttg ttcatagtgc ctgatgcgac gctttacgcg
    12421 tcttatcagg ccttcagggt cacggtatta atactcgtcc cgtccttcat cttcatccgg
    12481 ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcagacgct gcatatctcc
    12541 cagtaagcct aaatgtagcg aactggtttc aatactctgc acgttttgct ggtgcaggcg
    12601 atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aaacgatgtt tgctgcggcg
    12661 caaacgacgg gcgctggtaa catcgccaga gaagaaaacc gacattgcca gttttaaatt
    12721 actgagcaat tgctcataga gcgcatccag ttccttcaag ccatcaagcg aaaatgctcg
    12781 ccttgctgcc agcgatttgt cggcaatttc actccccatg cgctcgacga tatcggaggc
    12841 ctgttcaagg ttgagcgaca tttcgatgat ctccgcccag cggcgcgact cttcttctgc
    12901 cagttcctct tttggcatcc gcgccagata cagcttaatg gcggtataga gaacgttgat
    12961 gtcatcagcc agcttacgca gctctttctc ctgccgtggc tcgccgtgca tcactttatt
    13021 caacccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc gggcagcgtt
    13081 cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttag gccgtagctg
    13141 ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca tcgggtcaac
    13201 gaatggcaac atgaccaggc agcgtacaag gttgtagaag acgtggaaat agatcaccaa
    13261 ttccgctttt ggcaatggca acttccccat tgtctctgcc agcaaatgaa caaacggcag
    13321 gataatcagg ctacccacca gcttaaacag caggctaccc agcgcgacac ggcgggctgc
    13381 ggcattggcg gcactgttgt tgagcatcgc cagcaggccg gaaccgaggt ttgccccaat
    13441 caccagacaa agcgccactg ggaaagagat aatgcctgcg gcggtcaaag tcgcggtcag
    13501 cagtacagca gcaaggctgg agtagctgat aatggcgaac atcgcaccaa tcagcgcatc
    13561 cagcaaaatg tcgccagtca gcgaggcaaa gatcacctga acgccgtttg cctgagtgat
    13621 cggcgttacg gcctgcacaa tcaactccag cgccagtaaa atcagcccaa gaccaatacc
    13681 gacgcggccc agttgcccgg cgcgtgactg tttgcgtccg aggaaaaaaa tcacgccgat
    13741 aaaaataagt aacggtgaca gccaggataa atcgaaggtg agaatacgcg ccattagcgc
    13801 cgtcccgaca tctgcaccca gcacaatcac cagcgcaggc gcgagagcaa ccagatcctg
    13861 agcgacgaac gaggtcacca gcatggtggt ggcattactg ctctgcacca gtgcggtaac
    13921 gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa ggacagtacg
    13981 cagacgcgcg ccgaagacgc gcattacgcc tgttcgaaca atatgagtcc cccagaccag
    14041 cagggcgacg gcagaaagca ggtgaagcaa agttagcacg gaatcaggtt ctcctttttg
    14101 ttatgcattt tcctgttaag atttttctaa tcacgttgtt tttatccagg catttctctt
    14161 gtatgtactt tcagtataag agatttatac aagaaagcat ttaaaaaggc atttatataa
    14221 aaatccacct gtatctgttg ctatttctgt cacactttcc cctgtcgttt acaccagata
    14281 atcgttgtca cttttgtgac ctctgagaag ataaacaacg aaatatttaa gaccagattc
    14341 tgtgaatgaa aataattaag taacaacggt attacggaga ttgaacagct tacggtagca
    14401 aaacgcataa actcctgaat cgccaaaaaa gagaaactaa ataatttttg tcttaaaaaa
    14461 cattgcgcag aaaagaataa agagaacaat aatactattt gtagcgtaac agtggcgact
    14521 gcataagcgt aaaaaccgcc ttcaacaccg atatccttaa gtaaagaaag tgaatgaacg
    14581 atatcggaat aaaaagggat acgaccatta ccaagcgagt agaacagata tgtggctatc
    14641 gcgataacat ccataacacc ccaaaaaaga agaatgtatt ttttatattg acgttgcata
    14701 aagtaaatcc tttttacgaa aacaaatatt accaggaatt attataattg cgggcgtttt
    14761 tggattaaca ctccacccgt attttttaaa cctctgtcag ctataccttc actaaacaag
    14821 acatcgatac gccctttact tttatcgtga tacagaatac tgcatgagat acaccttata
    14881 ccctctttgg atttacga
//
LOCUS       DAZKHK010000119        14787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKHK010000119 DAZKHK010000000
VERSION     DAZKHK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.119"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            230..739
                     /gene="ybgA"
                     /locus_tag="ACHOS5_003900"
     CDS             230..739
                     /gene="ybgA"
                     /locus_tag="ACHOS5_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP9778643.1"
                     /translation="MNQQRFDDSTLIRIFALYELHRLKEHDLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            736..2154
                     /gene="phrB"
                     /locus_tag="ACHOS5_003901"
     CDS             736..2154
                     /gene="phrB"
                     /locus_tag="ACHOS5_003901"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP9778644.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVETVKQVCAENRITHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFMPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCLN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2196..3677)
                     /gene="dtpD"
                     /locus_tag="ACHOS5_003902"
     CDS             complement(2196..3677)
                     /gene="dtpD"
                     /locus_tag="ACHOS5_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP9778645.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            3948..4691
                     /gene="ybgI"
                     /locus_tag="ACHOS5_003903"
     CDS             3948..4691
                     /gene="ybgI"
                     /locus_tag="ACHOS5_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP9778646.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4714..5370
                     /gene="pxpB"
                     /locus_tag="ACHOS5_003904"
     CDS             4714..5370
                     /gene="pxpB"
                     /locus_tag="ACHOS5_003904"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP9778647.1"
                     /translation="MQRARCYLIGETVVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5364..6296
                     /gene="pxpC"
                     /locus_tag="ACHOS5_003905"
     CDS             5364..6296
                     /gene="pxpC"
                     /locus_tag="ACHOS5_003905"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP9778648.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6286..7020
                     /gene="pxpA"
                     /locus_tag="ACHOS5_003906"
     CDS             6286..7020
                     /gene="pxpA"
                     /locus_tag="ACHOS5_003906"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP9778649.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQLGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            7056..7847
                     /gene="nei"
                     /locus_tag="ACHOS5_003907"
     CDS             7056..7847
                     /gene="nei"
                     /locus_tag="ACHOS5_003907"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP9778650.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDTPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7844..8890)
                     /gene="abrB"
                     /locus_tag="ACHOS5_003908"
     CDS             complement(7844..8890)
                     /gene="abrB"
                     /locus_tag="ACHOS5_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9778651.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(9042..9437)
                     /locus_tag="ACHOS5_003909"
                     /pseudo
     CDS             complement(9042..9437)
                     /locus_tag="ACHOS5_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(9564..10847)
                     /gene="gltA"
                     /locus_tag="ACHOS5_003910"
     CDS             complement(9564..10847)
                     /gene="gltA"
                     /locus_tag="ACHOS5_003910"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP9778652.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(11053..11160)
                     /locus_tag="ACHOS5_003911"
     CDS             complement(11053..11160)
                     /locus_tag="ACHOS5_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778653.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            11541..11945
                     /gene="sdhC"
                     /locus_tag="ACHOS5_003912"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             11541..11945
                     /gene="sdhC"
                     /locus_tag="ACHOS5_003912"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9778654.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            11939..12286
                     /gene="sdhD"
                     /locus_tag="ACHOS5_003913"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             11939..12286
                     /gene="sdhD"
                     /locus_tag="ACHOS5_003913"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP9778655.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            12286..14052
                     /gene="sdhA"
                     /locus_tag="ACHOS5_003914"
     CDS             12286..14052
                     /gene="sdhA"
                     /locus_tag="ACHOS5_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP9778656.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            14068..14784
                     /gene="sdhB"
                     /locus_tag="ACHOS5_003915"
     CDS             14068..14784
                     /gene="sdhB"
                     /locus_tag="ACHOS5_003915"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP9778657.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
BASE COUNT         3590 a         3650 c         4046 g         3501 t
ORIGIN      
        1 cagccgcaag ggctttaagt ttcttttcgt ccatcgtttg cctgtctccg ttgttggagt
       61 gaacatatca aaaacaggca attacacaat tttaattaca gtctcaacct gacgtcagtc
      121 cttgcgggga gcgggctttc gtaaatttgt cctgctacaa caggattaac ttcacaaata
      181 tcatttctca acgtctacac ttactcctgt taatatctca ggagcagtaa tgaatcagca
      241 acgatttgat gacagcactc taatccgtat cttcgcgctt tatgagttac atcgactgaa
      301 agaacatgac ttaacgcgcg gggcgcttct cgattatcac agccgctata aactcgtctt
      361 tctggcgcat tctcagccgg agtaccgcaa acttggcccg tttgtggctg atattcaccg
      421 gtggcaaagt ctggatgact attacaacca gtaccgccaa cgcgtaattg ttttgctttc
      481 tcaccctgcc aaccggcgtg atcatactaa cgttttgatg cacgttcagg gatattttcg
      541 cccacatatt gattccacag aacgccagca gctggcggcg cttatcgaca gttatcgccg
      601 tggcgagcaa ccgcttcttg cgccgctgat gcgtatcaaa cactatatgg cgctttatcc
      661 tgacgcctgg ctttcagggc agcgttattt cgaactttgg ccgcgtgtga ttaacttgcg
      721 ccatgcagga gttttatgac tacccatctg gtctggtttc gccaggattt acgtcagcac
      781 gataatctcg cgctggctgc cgcctgccgc aattcgtctg cgcgcgtgct ggcgttgtat
      841 atcgctacac cacgccagtg ggcggcgcat aatgtgtcgc cgcgtcaggc tgaacttatc
      901 aatacacaac ttaatgcgct gcaaaacgcg ctggcggaaa aaggcattcc tttattgttc
      961 cgcgaagtgg atgactttgc tgccagtgtc gaaacagtta aacaggtgtg cgcggaaaat
     1021 cgcattaccc atctgtttta taactatcag tatgaggtga atgagcgggc gcgggatgtg
     1081 caggctgaga ggacgctgcg taacgtggtg tgtgaaggat ttgatgacag cgtgatcctg
     1141 ccgccggggg cggtgatgac tggcaatcat gagatgtaca aagtctttat gccttttaaa
     1201 aacgcatggt tgaaacggct gcgggaaggg atgccggagt gcgtcgctgc accaaaagtt
     1261 cgtagtagcg gatcgataga gcccgcgcca tccattacgc tgaattatcc tcgtcagtct
     1321 ttcgatactg cgcatttccc ggtggaagaa aaagcggcga ttgcgcaatt acgccagttt
     1381 tgcctgaacg gtgccggaga gtatgagcaa caacgagatt ttccggcagt ggaaggcacc
     1441 agtcgtttgt ccgccagcct ggcaacgggc gggttatcgc ctcgccagtg tttacatcgc
     1501 ctgttggcgg aacaaccgca ggtgctggaa ggcggaccgg ggagcgtctg gcttaatgag
     1561 ctgatatggc gcgagttcta ccgccatttg atgacgtatt atccttcgct gtgtaaacat
     1621 cgtccgttta ttgcctggac ggatcgtgta cagtggcaaa gcaatcccgc ccatttaaag
     1681 gcctggcagg agggcaaaac gggatacccg attgtcgatg ctgccatgcg tcagcttaac
     1741 agcactggct ggatgcataa ccggctacgg atgattacag ccagttttct ggtgaaagat
     1801 ttgttgatcg actggcgcga aggcgagcga tatttcatgt cgcagttgat tgatggtgat
     1861 ttggcagcta ataacggtgg ctggcagtgg gctgcttcta cgggtactga tgcagcgccg
     1921 tattttcgta ttttcaaccc gacaactcag ggcgagaaat ttgaccgcga aggcgaattt
     1981 atccgccagt ggctaccgga gctgcgcgat gtaccgggga aagcggtgca tgagccgtgg
     2041 aagtgggcag agaaaacagg tgtgacgttg gattatccgc aaccgatagt cgatcacaaa
     2101 gaggcaaggc tgcgaacgct ggcagcgtac gaagaagcga ggaaaggagc ctgataaaac
     2161 gcagcgagcg ttttatcagg catggtgctg gcttattaag actccagcgc cagcgcgcgg
     2221 tttctgaact taagcgcctg atacagccaa atcatcagta ccagtcctac acacgccagt
     2281 gcgccccagg taatttgatc aaatacttca atatatgcat tgatggagta gttgatcgcc
     2341 ccggaagcat caaacgaagc ctgcgatgtc tgatcggcaa tcacgcccgc cagatagttc
     2401 gcaatcgcgc cggaaagcag catataaatg ccggttaata cgccggtcac gccggggatt
     2461 tcaatgcgcg taatctgcga catagcaacc ggatcgataa acagttccgc aaagcccatc
     2521 accgccaggc ctaacaccat cagtggcaga gaagagtgac catacattgc ggaccagcgg
     2581 gcgcttaagg tcagaatgca gaatccggcg ctcatcaggc caaggccaag agcaaatttt
     2641 ccccagatgc gcacggtacg attacctgcg acgctttctt taaccaccca cgccaggaac
     2701 acaccgcaca gcataactgc gaaggcatta atcgactgga acatagcagt cggaacggta
     2761 taaccaaaca tatcgcggtt aacgaagcgg tcgatataaa ggctaatcga gctacccccc
     2821 tgttgtgcga atgcccagaa caacatgctg aagaaggtga gggtaacaat cagccccagc
     2881 tccttacgct gtttttggtt ttctgctttg cgataaattt ttgccagaac cccaaggcca
     2941 atgatcgtcg cgacaatcaa ggcgtatacc gaccactctt tccagaacaa cacggtaatc
     3001 agcgcaggcg ttgccaccag cagaaccagt agccatcccc agttgggcag gagaaagttt
     3061 gtcgcacgca gtaccttttt gttaacgccg cgggtatgtg tgaaatgacg attgccacat
     3121 aagaaaatga ccagacccgc gatcataccc accgccgcca ggccaaagcc catcgcccaa
     3181 ctgtactctt cctgggcgaa accacaggcg ataggtgcga taatagaccc cacgttaccc
     3241 gccgcataca tcagcgagaa accgccatca cgacgtggat cggttggctc atacagctcg
     3301 ccgagcaggc aactgacgtt ggatttaaac agaccatagc cgcagacgat aatcgccagg
     3361 gacagataga ggaatgacgg atggatctca ctggcaccca gcaccacatg accgatcgcc
     3421 atcaacaacg cccccagcat caccgccatg cgattgccga gaactttatc cgccaaaaag
     3481 ccaccgagga ttggcgtgac atataccagc gaacagtagg cgctaaataa ctcgtaggcg
     3541 tgattatcgc tatattttag ttgattggtg agatagagga tcagcagggc acgcatgccg
     3601 taaaagctaa aatattccca gatttgcagc gcaacgacat agtaaatagc gcgcggttgt
     3661 gatgcgtgtt tattcataat aattccacaa ttgactgcaa cgtcacgtaa tagttgcgcg
     3721 gcgtgcttgt gtttccttaa agttaaaact ttgatacaga tctgattatt tatgcaatat
     3781 gctgtctgat tgcataaata tacattagct gaagcgtgat gatataaaca atttgcgtct
     3841 tttggcgtcg ggtttgtttc ttaatatgtc aaaagattgg ctatcgacga acaggcgagg
     3901 taacgctatg ttaacggtgt agctatcaga atacggaagg cgcgaagatg aaaaacaccg
     3961 aactggaaca actgattaac gaaaaactga acagcgcggc gattagtgat tacgcgccga
     4021 atggtttgca ggtggaaggc aaagagacgg tgcaaaaaat tgttaccggt gtcactgcca
     4081 gccaggcttt gctcgatgag gcagtgcgtc tgggcgcgga tgcggtcatt gtgcatcatg
     4141 gctacttctg gaaaggcgaa tctccggtca ttcgcggaat gaagcgtaac cgcttaaaaa
     4201 cattgttggc gaatgatatc aacctgtatg gctggcattt gccgcttgac gcacatcctg
     4261 agctgggcaa taacgcacag ctggcggcgt tactggggat cacggtcatg ggcgaaattg
     4321 agccgttggt gccgtggggc gaactgacca tgcctgtgcc gggactggaa ctggcctcct
     4381 ggattgaagc gcgtctggga cgtaagccgt tatggtgtgg cgataccgga cctgaggtgg
     4441 tccagcgcgt cgcctggtgc acgggcggcg ggcaaagttt tatcgatagc gccgcgcgtt
     4501 ttggcgtgga tgcttttatt actggcgaag tttctgaaca gaccattcat tcagcccgcg
     4561 agcagggatt gcatttttat gctgcgggtc accatgccac tgaacgcggt ggtattcgcg
     4621 cattgagcga gtggctgaat gaaaataccg atcttgatgt gacctttatt gatattccta
     4681 atcctgcata acgaataatc agagggatcg aaagtgcaac gagcgcgttg ttatctgata
     4741 ggtgaaacgg tggtagtact ggaactggaa ccgccggtga cgctggctag ccagaaacgg
     4801 atctggcgac tggcacagcg tctggtggat atgccgaatg tggttgaagc cattcctggc
     4861 atgaacaata tcacagtgat tttgcgtaat cctgagtcgc tggcgctgga tgccatagag
     4921 cgtttgcaac gctggtggga ggagagtgag gcgctggagc cggagtctcg ctttattgaa
     4981 attccggtgg tttacggtgg cgcagccgga ccggatttgg cggtggtcgc ggcgcattgt
     5041 ggattgagcg aaaaacaggt tgttgaattg cactcctccg tggaatacgt ggtctggttt
     5101 ttaggttttc aaccgggctt cccgtatctc gggagtttgc cggaacaact acacacgcca
     5161 cggcgcgctg aaccgcgctt gcacgttccg gcaggttctg tcgggatcgg cgggccgcag
     5221 accggtgttt atccgctggc aacgccgggt ggctggcagt tgattggtca tacctcactc
     5281 agcctgtttg atccggagcg tgacgaaccc atcttattac gtccgggaga cagcgtgcgc
     5341 tttgtacccc agaaggaggg agtatgctga agattattcg tgcgggcatg tataccactg
     5401 tgcaggatgg cggtcgtcac ggttttcgcc agtcgggtat cagccactgc ggcgcactgg
     5461 atatgcctgc gctgcgcatt gctaacctgc tggtgggtaa tgacgccaac gcccccgcgc
     5521 tggagatcac gctcggtcag ttaacggttg agttcgaaac tgatgggtgg tttgccctga
     5581 cgggtgccgg ttgcgaagcg cggctggatg ataacgccgt ctggaccggc tggcgattgc
     5641 caatgagagc aggccagcgt ttaacgctta aacgcccgca gcacgggatg cgcagttatc
     5701 tggcggtcgc gggtggtatt gatgttccgc cggtaatggg atcatgcagc accgatctca
     5761 aagtggggat tggcgggttg gaaggccgtt tactgaggga tggtgaccga ctcccgattg
     5821 gcaaagcgaa gcgtgatttt atggaagcgc agggcgttaa acagctgctg tggggcaacc
     5881 gcattcgcgc cttgccgggg ccggaatatc atgagttcga tcgcgcctcg caggatgcat
     5941 tctggcgttc gccctggcag cttagctcgc aaagtaaccg gatgggctat cgcttacagg
     6001 ggcaaatttt aaaacgcacc accgatcgcg aactgttatc tcacggtttg ttaccaggtg
     6061 tggtgcaggt gccgcataac gggcagccca ttgtgttgat gaacgatgcg caaaccaccg
     6121 gtggttaccc gcgtattgcc tgtatcattg aggctgatat gtaccatctg gcgcaaattc
     6181 cgctcggtca gccgattcat tttgtccagt gttcactgga agaggcactg aaagcacggc
     6241 aagatcaaca acgttatttc gaacaattag cgtggcggct gcacaatgaa aattgacctg
     6301 aacgccgatc tgggcgaagg ctgcgccagc gacgcagaac tattaacgct ggtttcctct
     6361 gccaatattg cctgtggatt tcatgcaggc gatgcgcaaa ccatgcaggc ttgcgtgcgt
     6421 gaagcaataa aaaacggtgt cgcgattggc gcgcacccga gttttcccga cagggaaaat
     6481 tttggtcgca gcgccatgca gctgccgcca gaaaccgttt tcgcccagac gctgtatcaa
     6541 attggcgcgc tggcagcgat tacccgtgcg caaggcggcg taatgtgtca tgtcaaaccg
     6601 cacggcatgt tatacaacca ggcggcgaaa gaggcacaac tggcagacgc catcgccaga
     6661 gcggtatacg cttgcgatcc ggcattgatt ctcgtcgggc tggcaggaag cgagctgatt
     6721 cgtgcaggcg aacgatatgg cctggtaacg cgcgaggaag tgtttgccga tcgtggttat
     6781 caggcagacg gttcgctggt gccgcgaagc cagctaggcg cgttgattga aaacgaagaa
     6841 caggcgctgg cgcaaacgtt ggagatggtg caacacggca gagtcaaaag tatcaccggc
     6901 gaatgggcaa tggtcacggc gcaaacggtc tgcctacatg gcgatggcga gcacgcactg
     6961 gcgttcgccc gccgattacg agctacattt gctgaaaagg ggattgttgt cgcagcataa
     7021 ccccgcttaa taaagaatga aaaaaggata tcaccatgcc tgaaggcccg gagatccgcc
     7081 gtgcagcgga taacctggag gcggcgatca aaggcaaacc actaactgat gtctggtttg
     7141 ccttcccgca gttaaaatct tatcaatcac gacttatcgg tcaacacgtt acccatgtgg
     7201 aaacgcgtgg taaggcgttg ttaactcatt tttccaacga cttaacgctc tacagccata
     7261 atcagcttta cggcgtctgg cgcgtggttg ataccggcga agagccgcag accacgcgag
     7321 tactgcgggt aaaactgcaa acggctgaca aaaccattct gctttatagc gcctcggata
     7381 ttgagatgtt gactccggaa caactgacca cgcatccgtt tttacaacgc gttggtcccg
     7441 atgtgctaga tccgaatctg acgccggagg tggtaaagga acgattattg tcgccgcgct
     7501 ttcgtaaccg ccagtttgct ggattactgc tcgatcaggc gtttctggca ggacttggca
     7561 attatttgcg ggtggagatc ctctggcagg ttgggttgac cggaaatcat aaagcgaaag
     7621 atctcaatgc ggcgcaactg gatgcactcg cacacgcgtt actggatact ccgcgacttt
     7681 cttacgctac gcggggccag gtggatgaaa acaaatatca tggggcgctg tttcgcttta
     7741 aggtttttca tcgtgatggc gaaccttgcg aacgctgtgg cggcatcatt gagaaaacca
     7801 cgctgtcatc gcgcccgttt tactggtgcc caggctgcca gcactaggcc gaacgcttcg
     7861 gcgcataggt tgaaataaac cgtgcgatgg caggccccgt cagcaaaata ctgaacaggc
     7921 gcagggtttg catcgccatg atgagcgcca tatcggcatt gctccctgcg gcgatgaccg
     7981 ccacggtatc aagcccgccg gggctggtgg cgaggtaggc ggtcataaaa tcaatatgca
     8041 taaaccgggt cagcccccac gccatacccg cacaaatagc cagcagagca aaaatcgaca
     8101 gcaggatttg tggcagtggg cgcaatgccc gcagtaatat ttgcttatcg aaaccaagac
     8161 caatccgcca gccaattgcc atatatgcca tcgccagcag ccattccggg agttcgatgg
     8221 tgatgagctg accagactgg agcactgcgc ccgccagcat cgggatgagc atcgtgccgg
     8281 aaggaagacg tagcaagcat cccgccgtac cggcaacgac cgcgagcaaa atcgtaagca
     8341 ggagattaat gctcactggc gggaaccaga cgatctgctg gttaaccgct tcagcgttat
     8401 cgcccagcat catgcgggtg accagaacag cggctcctgc gacgaacagc actcgcagat
     8461 attgcataaa cgccaccagg cgaatatctg cgccgtaatc ttgcgccatt gcgaccattg
     8521 ccgccgcgcc gccaggagaa gaaccccagg ccccggtatt tccaggcagt gagctatagc
     8581 gcaccaataa ccaacccacg atggcgctgg aaagcagcgt caccagtaaa atcgcaagca
     8641 cgatcggcca gttgacggcc agcgtggtga gaatcgaacc agtgaggttt tgtgcaatca
     8701 tgcagccaag aatggcttgg gcagcgagaa aagcggagcg gggaagttgc agagtgactc
     8761 cgcgcatact gaagatgatc ccggcgatca tcgggccaag caggagtgcc gccgggagat
     8821 gtaaagcgag gaagccaata gaaaggagaa gtgacagaac acataacatt ccccactgca
     8881 atacaggcat tcttcgcttc ctcattattt tatctttggt gtatcgatac agcataaaga
     8941 aagctgggtc tggaaaaaat aacggtaatc aaggaaaagg ggaaaatcag caataatctg
     9001 aatacgttgc cctttccggc tgaaaagggc gtcgtgttaa atcagttata gttaatctta
     9061 aaaagtacca ctgtatcgaa gggacccgcc gcgatgggag tgccaggtgt ggacgataat
     9121 tcagccgtat aggttttttc gtagatcagc atatcactgg tgaaatcggc gtattcagca
     9181 tatttattga atgtataagg cgaggcgttt tcatccagta tacgtagctt cagaccatta
     9241 ccaatcaaca atgctttatc ttcttcaacc aacgtttttt cggtataaaa cgaggagctg
     9301 accttaaatc cgtcggtaca ctggtcattt tgtgattttg ttgtcttgat actgaatgtt
     9361 ttcgacattg tgtggcggcg aatatccagt aagttaaatt tacccaaatc aatcaccagt
     9421 gtttccggtg aaatattttt tcaccattat taatgggtat ttgcatctgg tttaattttt
     9481 acaatttttt atgttcagcc atataaaaag aacccgctct attaaaggcg ggtccggaaa
     9541 gtgaacggct tagcaatcaa ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca
     9601 tatcctgtat acagctgacg cggacgggca atcttcatac cgtcactgtg catttcgctc
     9661 cagtgggcga tccagccaac cgtacgtgcc atagcgaaga tcacggtgaa catagaagac
     9721 ggaataccca tcgctttcag gatgatacca gagtagaaat cgacgttcgg gtacagtttt
     9781 ttctcgataa agtacgggtc gttcagcgcg atgttttcca gctccatagc cacttccagc
     9841 aggtcatctt tggtgcccag ctctttcagc acttcatggc aggtttcacg cattacggtg
     9901 gcgcgcgggt cgtagttttt gtacacgcga tgaccgaagc ccatcaggcg gaaagaatca
     9961 tttttgtctt tcgcacgacg aacaaattcc ggaatgtgtt taacggagct gatttcttcc
    10021 agcattttca gcgccgcttc gttagcacca ccgtgcgcag gtccccacag tgaagcaata
    10081 cctgctgcga tacaggcaaa cgggttcgca cccgaagagc cagcggtacg cacggtggag
    10141 gtggaggcgt tctgttcatg gtcagcgtgc aggatcagaa tacggtccat agcacgttcc
    10201 agaatcggat taacttcata cggttcgcac ggcgtggaga acatcatatt caggaagtta
    10261 ccggcgtagg agagatcgtt gcgcgggtaa acaaatggct gaccaatgga atacttgtaa
    10321 cacatcgcgg ccatggtcgg catttttgac agcaggcgga acgcggcaat ttcacgatga
    10381 cgaggattgt taacatccag cgagtcgtga tagaacgctg ccagcgcgcc ggtaatacca
    10441 cacatgactg ccattggatg cgagtcgcga cggaaagcat ggaacagacg ggtaatctgc
    10501 tcgtggatca tagtatggcg ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc
    10561 ggtttttcac cattcagcag gatgtaacaa acttccaggt agttagaatc ggtcgccagc
    10621 tgatcgatcg ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat aaaagtaatt
    10681 ttagattcgc aggatgcggt tgaagtgaag cctgggtcaa aggtgaacac accttttgaa
    10741 ccgagagtac ggatatcaat aacatcttga cccagcgtgc ctttcagcac atccagttca
    10801 acagctgtgt ccccgttgag ggtgagtttt gcttttgtat cagccattta aggtctcctt
    10861 agcgccttat tgcgtaagac tgccggaact taaatttgcc ttcgcacatc aacctggctt
    10921 tacccgtttg ttatttggct cgccgctctg tgaaagaggg gaaaacctgg gtacagagct
    10981 ctgggcgctt gcaggtaaag gatctattga tgacgaataa atggcgaatc aagtacttag
    11041 caatccgaat tattaaactt gtctaccact aataactgtc ccgaatgaat tggtcaatac
    11101 ttccacactg ttacataagt taatcttagg tgaaataccg acttcataac ttttacgcat
    11161 tatatgcttt ttctggtaat gtttgtaaca actttgttga atgattgtca aattagatga
    11221 ttaaaaatta aataaatgtt gttatcgtga cgcggatcac tgttcgggat aaaacccgac
    11281 aaactatatg taggttaatt gtaatgattt tgtgaacggc ctatactgcc gccaggtctc
    11341 cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa
    11401 gcagtgtttt gcatgacgcg cagttataga aaggacgctg tctgacccgc aagcagaccg
    11461 gaggaaggaa atcccgacgt ctcctggtaa cagaaagtta acctctgtgc ccgtagtccc
    11521 cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa
    11581 gacctgttaa tctggaccta cagaccatcc ggttccccgt cacggcgata gcgtccattc
    11641 tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg catcctgctg tggcttctgg
    11701 gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct
    11761 tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc atatcacgtc gtcgtaggta
    11821 ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct
    11881 ccgccaaaat ctcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat
    11941 ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgtgc
    12001 taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg ctaccagtgg
    12061 cgagctgaca tatgaagtct ggattggttt cttcgcctct gcgttcacca aagtgttcac
    12121 cctgctggcg ctgttttcta tcttgatcca tgcctggatc ggcatgtggc aggtgttgac
    12181 cgactacgtt aaaccgctgg ccttgcgcct gatgctgcaa ctggtgattg tcgttgcact
    12241 ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc
    12301 agagaatttg atgcagttgt gattggtgct ggcggcgcag gtatgcgcgc ggcgctgcaa
    12361 atttcccaga gtggccagac ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat
    12421 accgtttctg cgcaaggtgg tattaccgtt gcgctgggta atacccatga agataactgg
    12481 gaatggcata tgtacgacac cgtaaaaggg tcggactata tcggtgacca ggacgcgatt
    12541 gaatatatgt gtaaaaccgg gccggaagcg attctggaac tggaacatat gggcctgccg
    12601 ttctcgcgtc ttgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac
    12661 ttcggcggcg agcaggcggc acgtactgcg gcggctgccg accgtaccgg tcacgcactg
    12721 ttgcacaccc tttatcagca gaacctgaaa aaccacacca ccattttctc cgagtggtat
    12781 gcgttggatc tggtgaaaaa ccaggatggc gcagtggtcg gttgtaccgc actgtgcatc
    12841 gaaactggtg aagtggttta ctttaaagct cgcgcgacag tgctggcgac tggcggggca
    12901 gggcgtattt atcagtccac caccaacgcc cacattaaca ctggcgacgg tgtcggcatg
    12961 gctatccgtg caggcgtacc ggtacaggat atggaaatgt ggcagttcca cccgaccggt
    13021 attgccggtg cgggcgtact ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg
    13081 aacaaacatg gcgaacgctt tatggagcgt tatgcgccga acgccaaaga cctggcgggc
    13141 cgtgacgtgg tggcgcgttc catcatgatc gaaatccgtg aaggccgcgg ctgtgatggt
    13201 ccgtgggggc cacacgcaaa actgaaactt gaccatctgg ggaaagaagt tctcgaatcc
    13261 cgtctgccgg gtattctgga actctcccgc accttcgctc acgttgatcc ggtgaaagag
    13321 ccgattccgg taattccaac ctgtcactac atgatgggcg gtattccgac caaagtgacc
    13381 ggtcaggcgc tgactgtgaa tgagaaaggc gaagatgtgg ttgttccggg gctgtttgcc
    13441 gttggtgaaa tcgcttgtgt atcggtacat ggcgctaacc gtctgggcgg caactcgctg
    13501 ctggacctgg tcgtatttgg tcgtgcggca ggtctgcatc tgcaagagtc tattgccgag
    13561 cagggcgcac tgcgcgatgc cagcgagtct gatgtagaag cgtctctgga tcgcctgaac
    13621 cgctggaaca ataaccgtaa cggtgaagat ccggtggcga tccgtaaagc actgcaagaa
    13681 tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag
    13741 cagttgaaag ttatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag
    13801 ttcaacaccc agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca
    13861 acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cgcatagccg cttcgacttc
    13921 ccggatcgcg atgatgaaaa ctggctgtgc cactccctgt atctgccaga gtcggaatcc
    13981 atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt
    14041 cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac
    14101 ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgc
    14161 gacatgatgc tgctggatgc gcttattcag ctgaaagaga aagatcccag cctgtcgttc
    14221 cgccgctcct gccgtgaagg tgtgtgcggt tccgacggtc tgaacatgaa cggtaagaat
    14281 ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt
    14341 cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat
    14401 gcgcaatatg agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc
    14461 gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggttgtatga atgtattctc
    14521 tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc
    14581 ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac
    14641 agccgcctcg acggtttgag cgatgcattc agtgtattcc gctgtcacag catcatgaac
    14701 tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tatcaagtcg
    14761 atgttgttgc aacgtaatgc gtaaacc
//
LOCUS       DAZKHK010000120        14727 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKHK010000120 DAZKHK010000000
VERSION     DAZKHK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.120"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(457..1218)
                     /locus_tag="ACHOS5_003916"
     CDS             complement(457..1218)
                     /locus_tag="ACHOS5_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP9778658.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLCEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCIFY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(1211..3307)
                     /gene="traD"
                     /locus_tag="ACHOS5_003917"
     CDS             complement(1211..3307)
                     /gene="traD"
                     /locus_tag="ACHOS5_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP9778659.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(3304..4092)
                     /locus_tag="ACHOS5_003918"
     CDS             complement(3304..4092)
                     /locus_tag="ACHOS5_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778660.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEALLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(4089..4571)
                     /locus_tag="ACHOS5_003919"
     CDS             complement(4089..4571)
                     /locus_tag="ACHOS5_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778661.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(4568..5161)
                     /locus_tag="ACHOS5_003920"
     CDS             complement(4568..5161)
                     /locus_tag="ACHOS5_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9778662.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(5158..5676)
                     /locus_tag="ACHOS5_003921"
     CDS             complement(5158..5676)
                     /locus_tag="ACHOS5_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP9778663.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(5673..6353)
                     /locus_tag="ACHOS5_003922"
     CDS             complement(5673..6353)
                     /locus_tag="ACHOS5_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP9778664.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(6332..7051)
                     /locus_tag="ACHOS5_003923"
     CDS             complement(6332..7051)
                     /locus_tag="ACHOS5_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778665.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPGILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(7055..7804)
                     /locus_tag="ACHOS5_003924"
     CDS             complement(7055..7804)
                     /locus_tag="ACHOS5_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778666.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(7801..8400)
                     /locus_tag="ACHOS5_003925"
     CDS             complement(7801..8400)
                     /locus_tag="ACHOS5_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFP9778667.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(8586..9578)
                     /locus_tag="ACHOS5_003926"
     CDS             complement(8586..9578)
                     /locus_tag="ACHOS5_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9778668.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEGIEKGRLEGIEK
                     GRQEGIEKGRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(9627..10187)
                     /locus_tag="ACHOS5_003927"
     CDS             complement(9627..10187)
                     /locus_tag="ACHOS5_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9778669.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(10203..10406)
                     /locus_tag="ACHOS5_003928"
     CDS             complement(10203..10406)
                     /locus_tag="ACHOS5_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778670.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(10497..10964)
                     /locus_tag="ACHOS5_003929"
     CDS             complement(10497..10964)
                     /locus_tag="ACHOS5_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFP9778671.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
     gene            complement(11643..13685)
                     /locus_tag="ACHOS5_003930"
     CDS             complement(11643..13685)
                     /locus_tag="ACHOS5_003930"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP9778672.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLSRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGCLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(13688..14392)
                     /locus_tag="ACHOS5_003931"
     CDS             complement(13688..14392)
                     /locus_tag="ACHOS5_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFP9778673.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
BASE COUNT         3418 a         4466 c         3424 g         3419 t
ORIGIN      
        1 aataaactat gttctggggg ctgtcagcat agccagtttc tgcatctcgc tgttccagat
       61 tctgttcaaa taaatttatt ccagcctgaa tgatttattc ctgtttatta aaacacggca
      121 tcactactgt ttcccgttaa tgacacctca ttaacaattt aaagaggaaa cacatcagca
      181 tggaatttct ccgtactgcc cttccgttct gggccctgtt ccttctgacc tgggtttgtg
      241 ttgtgtattt tttcatttat gaagaacctc gttgcccccc gtggatctat gacgtcatca
      301 gggccatcac tctgttcatt attttcgtca acattgttgt cattccggcg ctgtcgttac
      361 tctgacactg acgcgcactg accgtaaggg cgtggcttgt ctgccgacac cctgacacaa
      421 aacaagtttt gcatcagggt gtccggcctc tgtggattat aaatatttct tgaaggatgc
      481 catggtcacg gtgatggcca cccccagcag catggccgcc ggccatatca gcaggttcgg
      541 atacaccgac gtcggccagg acaggtaaaa aatgcaggga atatatatcg ccggtttaat
      601 agactttttt gcatggtgat aaacaaagct ggactcatac gcagcaccat accggcgcag
      661 atcacgacgc cccagtccct ccacaaccgc gatcagtatc accatcacaa acagcggaac
      721 agacaacacc aggatcgtca cccgtatcag tgtgaccacc gtgacatata ctgtggccat
      781 caggtactca cacagataat ggctcagcca gcgccccaca tcctcactgt actgtgacac
      841 actgttaccc accacggcac cggcattttg ctgcttcagc cacgacagaa aacctgtttc
      901 cacaaacagc cactgatacg ccgttgctat ccattctgtc accgttaccg atggtgtcga
      961 cagcagcaga ctgcgggtga attccgaaga cagccacccc agttctgtct tcatcactgc
     1021 ctcactgtgt gctgccccct gctccggcca gaaaaaagca atcccgatat actcaaccag
     1081 caggctcagc agcagggagg aaaacaggat cccgaccagc atccacggcc agccccacaa
     1141 cagattccag agcagtccgt gctgacgtgg cggagagggt tgtgctgctc tgttcacctc
     1201 acgacggggt tcagccattc tgctctcctt ccggcatgtt cacggaggga gtctcattcc
     1261 accatccctc tccggtgtgg taattacgcc gcatggcctc tgcaattttt gcaatactct
     1321 ccggcatcag cgcgtcatct gcatcagttg caggcagggg catacggatt ttccacaact
     1381 gtcccccttc cagcagagca aaggcctgtc ctttcggcag ggtcacgata tctgccggct
     1441 ccagcaaagg caccttcaca gtaccaacac gatcctgggt actggatgtg aagtcctggc
     1501 cggtcgtgac atctgcggta tcctgatgac cggaaaccag cgttttggta taaatttcca
     1561 cctgggacaa ctgggtcgtc agtaactcgg ccgtccggtt ctccctgacc cgcagcataa
     1621 tcatgttatt aaagttaccc tgcacctggg ccgttttggc agcactaccg gtacgggcct
     1681 caatatcaga agaggtctgc gtgtaagccg tcacctgcat accggcaccg cctcccttgt
     1741 tgattaacgg aatgaactca tcgcccatca gttcattaaa ctcatcacaa tgcaggttaa
     1801 ccggattttt ggtaccattc cctcccggaa gaccggcatt cattccgtgt ttgtagatat
     1861 gcccggcgac actcaccaga tccgcaaaca tggagttacc cacggcactg gccacctcac
     1921 tgtcactcag cgcatcaaga ccaatgtaca ccaccgcctt tttacgtatc acctgctccc
     1981 agtcaaacac gggacgggta tcgctcatat ccaggtagtc aggtgccagc agttctgccg
     2041 tctttcctgt ggtcagtttt tccagcaacg gcaacaggga tgccacaatt ttgtcaaaat
     2101 aggtacgatc ataacgtacc gcgcttcgca gaccatccag tatcggatca tagagtttct
     2161 ttccggcttc actgctgaga gccacctcaa tggcccagat acgcacagca tccggctggt
     2221 tctgcatggt ccggggaaca tccgcttccc ccagtacatt catgttattc agaatggcgt
     2281 tttccagttc cggcatctgt tcgctgatca ccttaccagc ataacggata taaaggtcgg
     2341 caatattatt cacataacgc ataatcagcg tgtaatccgg gcgctccccc agtgccacca
     2401 gtgcacgggc aacaatgttg acaaaccgcc atgcaaactc acggaaggcc gcactgttac
     2461 cttcaccgga taactgaccg gccacacggg aagccacctc cgacacacga ccaaaacgcc
     2521 ccacggcgtt ataacgcgca gagatttccg gccagcccag atgaaagata taaagctcat
     2581 cgcccctgcc ggcacggtgt gcttcagccc agacacgcag caacagatcc gcatcccctt
     2641 tgggatcaaa gacaatggtg acatctcccc gacgaatatc ctgtgtgacc agcaactccg
     2701 ccagccgggt tttcccgaca cgggtggtgc catagacaat ggtgtgcccc acacgttcac
     2761 ccagtgccag cgtcacatcc tgctcatccg gctcaatacc atgcagggca ggatttcctc
     2821 ccaccggcgg caggggacgg acgggattca gcggcgagtc acgacgaaga agctgcccaa
     2881 tccagggaag actgtgctcc gttttcattt caagccagcg cgctgtccgg tacagactgc
     2941 ccggctgtag atagtgtgca acctccggtc tcagcgtgtc ctgaaggcgc tgggtgtgct
     3001 tctgtgtcca gcggaagccg cgtcccagaa acagccgctg acggctgacc ggaatttgcc
     3061 gggaactcat cacataacgg ggcagccggc gcagattgcg acggtaacgg atgatcttca
     3121 ttccctgccg gaaacggacc actgccagaa cgccaaagcc ggcggctgtg acatagctga
     3181 ccgaaggcgc gagcgccacc gcccagggcg ctgtcacaca gacaccggcc gccgttgctg
     3241 ccaccacggc ggtattcagt tccacagccg gacgcagcag agcctcaatc acataacggt
     3301 tactcatgac cgggtacctc tctgtaaaag acgccgccac catgagcgcg gcaacgcttt
     3361 ctccggtgat gctgacagtg cctccagtct gcgctgtaac accatcacct cccgccatgc
     3421 gtctgccgca ttcagcagta atgccttata caccagtggc ccgtcatgtc cgccggcatg
     3481 cacatcatcc actgtcaaca gcgcctctgt tctgaaacgg tccactttcc agcaggccag
     3541 gtcttcgcgg gaaacacact gccgccggat ctgcagacga agatacacat catgccggga
     3601 caacggttct gccaggttgt gaatttcccg acaggcagac cggaacgcct ggtctgtgtg
     3661 ataaatactc tgggccacat tgtgaagctg tgtgcgcccc tccccctgca ggtggaacag
     3721 aaagcgggcc agacagctgc ggtgattacc gtcagcactg acataccagt gaatgccatc
     3781 cgtgctgaca aaacccatcc cggcatgctg ttgctctgcc tgcgtgtaat atcccggatt
     3841 cttttcaagt aacgggatat tgatgtccat gcgtttacca cgctccagca gctccagcca
     3901 gctcattccg gcatactgtg gatggtcagt ccccacaatg cggtacacat tgatactgcc
     3961 ctgtcccgtc cagtagtgtt ccgtaataaa atccgacatt gccgcaaact ctgcggtatc
     4021 ccatgcaacc agttgttcat gtgcccaggc cggagcattc gcctgtatcc gctctgtaaa
     4081 tgcccccttc attcagctct tctctcctgc acttttgaga taccgatacc gtcaggccac
     4141 ggcataacag ccgggccccc cctgccttcc ccggatacgg ctgcagaacc acaaccacga
     4201 aacagaatga ctgacgagac gccttccata aacaaagcgc ccagcaacag aaccactgcc
     4261 tgcattttca gttcacgaat atcctcagca acagcctccc cgaaagaaat cgctgttcgc
     4321 ggacattcct gctcatcgac atacatacct gagacggata tgacggagga acatttttgt
     4381 accatcacat gcccttcagg cgcatcacgc cagcgggatg actccgtcgc ataacgctgt
     4441 gtcatgcctg cctcaccacc actcagattg ttccagacat acagcattac cagacatcct
     4501 gccagcattg acggccagac aatcagaagc cggagcagca gcataacaaa ccggtacagt
     4561 gtcataatca tgatcttcct ttattactca gtaaggtgcc attcagcagt tgcaacagac
     4621 gttgtccact gatcagctca atatccggcc acagtgtcag ccgtgcccgg ctgatgttcc
     4681 ccgtacgccc cgtatggata aaaaagcccc tgcatccctt ccggcgcagc agttcgccaa
     4741 aagcatcaat atgtgccgga cgaatggttc ttccgtaacg tttagcctgt atcagccagc
     4801 gttcaccgtt aatcaccacc tgaccatcaa gaccaccatc gccgctgtat gaggggttac
     4861 ggataaccgg catcccctgt tgctcaaaag ccagcagcaa cagttcctca aacacatacg
     4921 gactgatttt gcgcagatat ttcagccgcc gggcatcacc ctgcaattcg ttcagccgct
     4981 tcattacccg tgaggccgtg gcacgataac gctgatgccg gcgtgttctc acacacgctt
     5041 tttttcgccg gctgcgccac agtgccggcc ccaggacaac agccagcagg ctgagaacca
     5101 ggcaggctgt cagtaccgga gaagcagaga gcgcagcaga agtggcagac agcggcatca
     5161 tggtgcaggt acctgtgaca ggccgttgtc cgttatcagc accgggtaat gacttatctg
     5221 taacctgcgg gccagtacag aaccggatgc cggaaccatc accacccccg gtgccagctc
     5281 acgcagtacg cgcagactgc tctcatccgc cacattcact accattcccg ccgccttccg
     5341 ctccctgagt actgctgcat tcatctgcag ccatgtgcgg gaatgggcat catcgcccac
     5401 aagaaaaagt gcaccgatgc ccggtaactg aagccggcgc gccacaacct ctccgggtga
     5461 catttcaggc gtactgaccg gtaacattgc cgcctctccc tgcggaaaag agggtgatgg
     5521 cacaactgaa gcgggaaccg gtggctgccg gtttatcgcc tcaaaaaagg ggctggcatc
     5581 cctgccgccc aggtctgcaa tcactttcag ttctgcgcca gctgttgtac agaacagcag
     5641 cagactcagt acataaaact gtctttctcg tttcatttct gctcctcctg tggttcagtc
     5701 cagatataac ggggggatgg tgtcagcatg gcggtcgctg ccacatgcac atcagacgga
     5761 ttcaggaaat tatcccggtg gagccgggca agatgccgtc tgaccacggc ggtataacgt
     5821 gcggccggcg caccacctgc cgggtggtga taacacccgg ccacctgcag ccaactgacc
     5881 ggatggcgac gccggcattc ctgcagaatg accgctgccg tattcagact ggtgtaagga
     5941 tcaaatgccg cccatgtgga cgggaaacga tgtccattcc agcccagatt gacctgtgct
     6001 atcccgacat caatacgttt tgccggataa tgtttcagga acacctgcaa tgcctgccag
     6061 gcctccagac gtgtggcata acggtaccct tttcctgcca catttattgt ccagggccag
     6121 ggtcgggcca cgtccggctg acgaaaatca cgacgccgtt gtttcagtac tccggacatc
     6181 gcttccggag acatggacgt ttccgtcagg gaaacggcat acaaaagctc tgcgggaata
     6241 ccatgggcac gggccacccg cacatacccg tccggtacgt tctgaacggc agcacgacag
     6301 acaccactgc aggccaccat cagcagaacc gtcagaatgc cgccacgcgc catccgtcag
     6361 ccccctgctg cagtaccacc ggcatttttc cttcaccaaa acgcagccag cgtccggcat
     6421 catgattcag ggtgatatga cggctgcgaa ctttctccac aggtatgctg tgcgcatgcg
     6481 cccactgacg cagaatctca tcctttccct gactgtcgac aaggtagata tcaacctgcc
     6541 gtcccgtggc cagtacggag gcaagacggg catcacaggt gccacattcc ttcgccttca
     6601 caaacagcgc cagccgtccc cccgtatctc cggtaagtgc tcctgttttt cccatactga
     6661 caggcaagat accgggatac aggcgcttcc aggccgcatt cacctcctgc tgaaacgcca
     6721 gctccttctc tgttcgttca tattcagccc tgacccactt ttcggcccag acacgtcgct
     6781 ctgccggcgt tctggcctct ataccgagta ccgtcagcgg atcaagtccg ggagactgta
     6841 tcccccgggg gccggacatc agtgactgat aacgccggta atcctcctca ctcacccccc
     6901 actgcccggc ctgctgctga agtccggatg tggtactctg cgtcaccacc gtaccggtcg
     6961 tccgttgcgc ctctgacgac atattcgtct gcccggcgga ctgagccaga acggggcaca
     7021 gtgccagcag atacagtatt tttcgcttca tcatctactc caccctgagc accgtctgac
     7081 gaccattcac ccggaacgtt gcttcaccat ggccggcata cacaagcgtc cacccggcca
     7141 cactctctcc ttcacccagc agagccacct gagagaggct ggtatacccc cggggagcca
     7201 cggctgcacg ggtactgcca ccgcgcagct ctgtcccggt cagcacaaaa ggagcctgcc
     7261 gaactgtatg aactgcgcgg gatttacggg aggacgtcac cggagcgggc tttttctcct
     7321 ccgttttttt catccccgct ttcctgctgt ccggctgtga cggtgactcc ggtactttct
     7381 gcattttctc caccagaccg ctgagtgtat ccagtctcag ggaaagcttt tcccgggcag
     7441 actcaatctg ctccagcatc tccggcagat tctccagttg ttccttccag gatgctgtca
     7501 gcacgggctg ctgctccagg actgccaggc gcttttccat atccttcagt tgcttctccg
     7561 tctctgtcag tttcattctc aggggcgcga gttctgacgc agaaagcatt tctcttctct
     7621 ggccttccag ggtcgtgatc cggctgtcaa gaccggtcat aagcatcagt acgccatata
     7681 ccactgcccc catgaccagc cccacagtga cagacagtat caacacgccg gggaaacgac
     7741 tttttcttcg ctccggcgac ataccagacg caccctccgg tatctgcggc tgtttctcac
     7801 tcattttttc agaaaccctc cggttgcctt cacacccggt tcacgaccca caaaaggaac
     7861 ggtgaccgga acaggagcca gctggctgac cggtaactgg taaccgtcac gcagactgtg
     7921 gcagaccacc ctctgcacat catccacttc cagttgccat gccggccccg ccattaactg
     7981 cagggcagta cgcagtctca gtggtcccgg ctgatactgc accgccggca gaggctgacg
     8041 gtacagcacg ccgttggccg gtccggtggc gcagagcgtg taaccggact gtttcagggc
     8101 ataacgcatg gcatcggcca ccgtgggttt cagggaggag ggaatgcgaa tgtcgatcac
     8161 ctgagaaagt ggatcacgct gtacagcctg cggctctgtg ctgaccagca ggtaacggtc
     8221 atagcgcacc acctccggtg cctgagaata cacatccggg ctggcaggct gtacattacg
     8281 gtttactgtg accgacggta ccggcatggc cgcatcccgt tgctgtagtt tctgagtgct
     8341 ctgacatccc gccatcagga ggcagactgt cagtccggac aggataaagc gttttttcat
     8401 cgggtattta cctctgcagt gataaatcag aggtcaatgt gcagaagaag acggggctgg
     8461 ctcaatgaac aactcattac ccgctggcga aaaaaaacgc ctgcagacat gcaggcgctt
     8521 tgttgacagg aaagaaagca acataacaaa tatgtcacaa tcctgcggtt aacataaatt
     8581 accgattacc ggagagcatt acgcaaatca tcatccgaga actgcagtat ttgtttgatt
     8641 ttctctgacg atatacccat ctgctgcatg cgacgggccg atgcgtaatt ctcctgaaga
     8701 gccttttctc tctcctcctg gcggcccttc tcgatacctt cctggcggcc cttctcgata
     8761 ccttccaggc ggcccttctc aataccttcc tggcggccct tctcgatacc agccagttcc
     8821 agtcgttcag cgatagtcat tagatcatcc tcatgtcttg gcagacgttc tgccatcgcc
     8881 cgtataaatt caggtgaagc tgaatcacat tctttcacca tatagttaaa caatgtcacc
     8941 acctgagtgt gtgtttcctg tatctgaatc aactgtgaca aaaagtcaag atgaaccatc
     9001 agatccactt tcttctgccg gagcttcatc ataagcatca gcgcagccat ctgcttatag
     9061 cgacggatac cctcatcatc aagaagaccg acatcaatca ccctgggtgg cctgccttct
     9121 ccataaagct cccgaccaaa cgccggatca tccagacagt caggccagtt catgctgtat
     9181 ggataaggac tgggtacacc atggtaaaaa agcaccggta tcacaacggg agcacacgca
     9241 tgtgctttca ggtggcgatg tatcaccgcc agaacatagc gcatcattcg cagggccata
     9301 tacttatccg ccttgctctg gtgctctgtc agcgtataaa tgtatccatc cctgccatct
     9361 gtggttttca tactccacag aacatcacag gcaaactggc gaagatccgg atccacaaac
     9421 gtggtcggct ccagttgcag ggtgttcata tcgcaccggt tcagttgttc cgggggcaac
     9481 gcgcctttca ggaaatccct cgcaacagaa ggacattcca tcatcgctct gaacgccgca
     9541 tcatgtggcg tgggggtagt gggtgtgttt tcattcacaa ccgcctcctt gaattaaaag
     9601 tggggcggta agccccgtgg ttaacatcag atgacatgga taatccgtcg ttcaacgcca
     9661 tacccacgcc ccataaaggg gatatcatcg tcaaagtcca tcggcggcat tcccccgctt
     9721 cctgaggaag cctgaccgtt ttttcccgtt gattgttgtg cattttgtgg tggttgtccc
     9781 catcctccct gagcaggagc agatgaaaca tttccaccac cctgctgctg acgcggagac
     9841 cccagcatct gcagggtacc attgacactc acaacaactt ccgtggaata acgatcaaca
     9901 ccattctgct gatcgaacca cttccgggtt cttaactgac cttcaatata cacctgtgaa
     9961 cctttacgaa ggtattcacc ggcaatctca gccagttttc cgtaaataac aacacggtgc
    10021 cattcagtgt gttccttcat ttcaccgctc tgtttatcac gccaggtatc agtggtggcc
    10081 agcgtcagat ttgccactgc acctccgccg ttggggaaat aacggatttc cggatcctgt
    10141 cccagatgac caatcagtat gactttattc actccacgcg atgccataac aactccttat
    10201 cctcacacac attcacgctg agtccagatg ccgttttcca gagcccggac tgcctgctca
    10261 taatttctga aatactcttc ggattcccgg gacacaggcc cttcgtcgca ctgagtacca
    10321 atataataac cacgagcact gctgaggatc tgcagaggta aggtatagcc cgctttggcg
    10381 gccagaattc cgcttgtcgg tttcataaca tgtttcctta aagttaaacg ataagaaaac
    10441 agacctccct gatggttggt ctgttttcat cagggaggtt gagttgaaaa atacgatcag
    10501 aaagaatttt ctgcgtattc ccgcggggta ttgccattct gtggcggtat ggattccgat
    10561 ttttcagtct tataaaccat gtcctggcct attttgatcc actccacctt gatcaggcgg
    10621 gctttcagac tgacgcgttg ctcaccggca tgttcacccc ggttaagggt aaaaatgtca
    10681 gtagaggggt tgctcatggt aaaaccaatc agcaccttgc gatcttcatc caccgctttc
    10741 tggcagcggg caatcaggga agttgcctct ttaccggcaa ccgtcacatc aaaacgcaca
    10801 taggacgggc tgtccgtcgg accactcagt gcattcacaa cacaactgag aaatgtcccg
    10861 ttctgatgat taacctgacg aatgttgctg agatacccca ggcctttcag tgtcagattg
    10921 aagtactcag ttttagcgtt tgcagaagaa gtatttgcag tcataatgat atctccattc
    10981 tcagtatcgg aaagacagag aacatcatca tccctgacgg gttgtgatat tcccgtcagg
    11041 gagccggaac agaactattc cggctgactc agtaacagtt aaagaaaacc ttcatcgtca
    11101 tgactgacga tatctgctct caaaggtggg cagatttacc accaccagaa acatcctggc
    11161 tgcacatccc ctttcaggtt acgggggtgt tgatcacccg aaggcgttcc tctcagatca
    11221 acggaacatt ggtgcccacc gaagcgggat gtggtcaccc gaaggcgctc ctctcagacc
    11281 acttatggag cattggtggt cgccgaagcg acatgtagcc acccgaaggc gctcctctca
    11341 ggccacttgt ggagcattgg tggtcgccgg agcgacgtta tgtgcccgga cagactgaaa
    11401 tcactgatgg aaaaagtcaa cagctaacca aaaacaaaaa agcaccggca agccggtgct
    11461 tcagggggat accaaatccc ggtgttgatt ctcatcttct ccggagagag cgactcccgg
    11521 cacggttttc acggagtgca tgtgccttgt aaccccgccg tgtgcataac gggttaccgg
    11581 acctctcagg gctgtcagcc ccactgtggt caacagggtg ataccagaac tcgcctgcaa
    11641 ttttaattca gtttgtctcc gacatcacca gatttttgtt tatgagaaaa attaattttt
    11701 cttttcctgc ccctgctgtt tccctgctca ccagcaattc ccctgcagtc gggataacgg
    11761 gagcatcccc agaaaaaacc gctttttccc ttccgtttca ccgtcggacc gttacagagt
    11821 ggacatgaag gacctgattc cactgacaga ttaaccgtct ggttacgtcc ggataacacc
    11881 aggtgcctga tccactgatg ctgtttctcc ataaacgcct ccagtgagat tttcccccgg
    11941 gcaacatcat ccagcgcctg ttcccataaa gccgtcatac ccggatttgt cagagagtcc
    12001 ggcaatgccg ccaccagttc tcttcccagt tgcgtggaac ggatatgttt tcccgctctc
    12061 tcaatatagt tgcgcgcaaa taacgtttcc aggatccccg cgcgtgtggc ttccgttccc
    12121 agtcccgcat tctccctcag gatctttttc agcgccggat ccgttacacc agacgaagca
    12181 ttcttcatcg cagtaataag acagccttca gtaaaaggac ggggaggtac ggttttcagc
    12241 tccttctgtt cagcaccatc cagagcacag agttctccct ttatcaaagg gggcaatatg
    12301 tttccacttt catcactgtc aggcttttct tcatctgcat gattttccgg tggaaataac
    12361 tgtttccacc cgataatcac attaactctt ccgcgggaac ggaataactg ccccccaatc
    12421 acaaatgttg cctcagtaac atcagcttcc tgctccggca gaaactgtgc cagatagtgc
    12481 tgacgaatca gttgatatac cttcagttca tcaccgttca gtgagctgag gtcaaaaacc
    12541 tgccgggtcg gaataatggc atggtgagcc gtaatttttt tatcattcca gatacgggat
    12601 acccgtcctg attccagttc agccagttgt ggaagcacgg acggatctga acgcccgaca
    12661 gcctcaagca cttcagaact ctcctcatgc atcgaggcag gcagataacc acagtctgtc
    12721 ctgggataag ttgttgcttt gtgagtctca taaagggact gtgcgatatc cagaacggtc
    12781 tgagccccca tgccaaactt ccgggaacag gcttcctgca acgttcccag cgaaaaacac
    12841 aaaggtggcg gtgttttctc cctcttctgt acaacatcct gtacaacagc agaggactgc
    12901 tgacggcaca gaccgagaac agcaccggcc gcctgcacat tcacacaacg tccctcatca
    12961 tcgcagtact gctcagctgc cacccagtta ctgcggaaac ggagcccctc cttttccagc
    13021 cagacaatca cctgccacca ggaaaccggc acaaatgtgg cgatctcctg atcccgtctg
    13081 accaccattg ccagcgtggg tgtctgcacc cggcccactg acaaaacccc agaaattcca
    13141 cgttcccgtg ccttcagggt atacaaccgg ctcagattca tccctgtcag ccagtcagcc
    13201 cgggcacgcc ccttaccggc ttcactgaga agcgcagtct gttcgcccgg gagaatatta
    13261 ttcagcgcct tcctgatact ggcctcatcc agtgcagata accacagacg cctgacacgc
    13321 cctctgtact gacagtactc cagcaactct ctggctatca cttctccttc acgatcggca
    13381 tctgtggcaa taaccacttc agaagcgcgt tttaacagcc cccggatcac aataagttgt
    13441 tttttcacct tcggcttcac caccatctgc cattgttccg gtatcagcgg caacacatca
    13501 gaacgccatg gggaaccgaa ctgttcaccg taagcttccg gctcagccag ttccagcagg
    13561 tgtcctatcg cccaggtgac agcaatattg tcatctccga tgtatccgtc gccccgctta
    13621 ctgagcgcca gttcacgggc gatatctttc gcctgagaag gtttttcaca cagaaagagt
    13681 tgcatactca ccccgcttta accggtggtg aataaacaga acgtttctta ccgctcatca
    13741 cgtcaggatc cggctccccc atgcggttaa ccgcctcctt tccacgggct gtgccggaca
    13801 aaatatcagc acgtgtaaca ctgactgatt tataattttg aataatcccg taaatcctgc
    13861 gtaccgcata ccctccctga ttcaacaact catcccgttt tgtgcgggaa ataagtccgt
    13921 aatgaaatac ctgaaatacc ttcatgacca gtcgatcata tccgatcaga agccatacac
    13981 atcgataacc gaccggagaa ctactgtaca cgccaatatt taccggcgaa gaggatttaa
    14041 tatcagaaag ggagatctgt cccggcaggt ttttaaggat cccgtcaagc cacgcaagat
    14101 gcttttcaat tgtttcagtt gccttcgtca gggcattttc cagcctgact aacatcatat
    14161 ctgcataagg attatccctg gctgaatctc tggttgcctg acccgcaaac gcaatagccc
    14221 tgggcatact gatgatgtca ggccgctttt cctgtttttc ctccttctga ttattcctgc
    14281 gtccctccca gagacggatc gcatatcggg tatgaagggc aatagtcagc gatgactgca
    14341 gagcaccaac ttgttctgat tttctgactt ctccggtttt ctcctggggc attgtgttta
    14401 ctccaaatga tttcatgacc agaagatagt ccgcaggttg cgtattaccg acaacaaaaa
    14461 aacaaatcag gcagaatata catttaacgc cgattatttt taatcgctgt tgacactggc
    14521 aacaccgctc actttttaac ctctgttgcc actggcagca ctgttcactt tttaacctct
    14581 gctgccactg gcagcactgt tcgcttttta acctctgctg ccactggcag cactgttcgc
    14641 tttttaacct ctgctgccac tggcagcact gctcattttt taatctctgc tgccactggc
    14701 agcactgttc actttttaac ctcttct
//
LOCUS       DAZKHK010000121        14610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKHK010000121 DAZKHK010000000
VERSION     DAZKHK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.121"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            176..382
                     /locus_tag="ACHOS5_003932"
     CDS             176..382
                     /locus_tag="ACHOS5_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778674.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            935..1429
                     /locus_tag="ACHOS5_003933"
     CDS             935..1429
                     /locus_tag="ACHOS5_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9778675.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1429..3531
                     /locus_tag="ACHOS5_003934"
     CDS             1429..3531
                     /locus_tag="ACHOS5_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9778676.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRIMPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSHSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            3528..3740
                     /locus_tag="ACHOS5_003935"
     CDS             3528..3740
                     /locus_tag="ACHOS5_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778677.1"
                     /translation="MNQNDIEAMIQRYMEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3740..5248
                     /locus_tag="ACHOS5_003936"
     CDS             3740..5248
                     /locus_tag="ACHOS5_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9778678.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFVQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKDYEDYELTAARIAAALGMYIRKG
                     DGQSYEPDGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            5193..7220
                     /locus_tag="ACHOS5_003937"
     CDS             5193..7220
                     /locus_tag="ACHOS5_003937"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9778679.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7307..7630
                     /locus_tag="ACHOS5_003938"
     CDS             7307..7630
                     /locus_tag="ACHOS5_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFP9778680.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7623..7898
                     /locus_tag="ACHOS5_003939"
     CDS             7623..7898
                     /locus_tag="ACHOS5_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9778681.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQHWTVTRHERFNGKPMIFIE"
     gene            7910..8488
                     /locus_tag="ACHOS5_003940"
     CDS             7910..8488
                     /locus_tag="ACHOS5_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9778682.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLTRR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLVNGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8485..8886
                     /locus_tag="ACHOS5_003941"
     CDS             8485..8886
                     /locus_tag="ACHOS5_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9778683.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8897..9640
                     /locus_tag="ACHOS5_003942"
     CDS             8897..9640
                     /locus_tag="ACHOS5_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9778684.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEICAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGSETVG
                     VTGLTVAPASASINVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVATVAVSAAG"
     gene            9701..10087
                     /locus_tag="ACHOS5_003943"
     CDS             9701..10087
                     /locus_tag="ACHOS5_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9778685.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAGEG
                     ELSEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDLEHEALTPEKS"
     gene            10096..10425
                     /locus_tag="ACHOS5_003944"
     CDS             10096..10425
                     /locus_tag="ACHOS5_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9778686.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            10397..13468
                     /locus_tag="ACHOS5_003945"
     CDS             10397..13468
                     /locus_tag="ACHOS5_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9778687.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKSAQAHERMAREVEKTRQRMEALSQK
                     MRDEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSSGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSYMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKAVKGIGSALGMGSVLGSLSLNADGGVYHSAD
                     LSRYSGSVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGIAMFA
                     PQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGQLSGAYR"
     gene            13468..13797
                     /locus_tag="ACHOS5_003946"
     CDS             13468..13797
                     /locus_tag="ACHOS5_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9778688.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHQSLKAFLEQHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13807..14505
                     /locus_tag="ACHOS5_003947"
     CDS             13807..14505
                     /locus_tag="ACHOS5_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9778689.1"
                     /translation="MQDIPLETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLAVSNLYGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWSYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14510..>14610
                     /locus_tag="ACHOS5_003948"
     CDS             14510..>14610
                     /locus_tag="ACHOS5_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9778690.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERY"
BASE COUNT         3554 a         3382 c         4383 g         3291 t
ORIGIN      
        1 cgattgtcga tggtattggt tgtgtgacaa tacctagtct ttccggtatg cctgaagaga
       61 atacaaacga cagattatgt aagggaaacg cgctgaaact ttcactcttt catgctgagt
      121 gatttgacgt tatattttta gccgcaagtg aagagcaaac gcatggagcg acaaaatgca
      181 taaagatcaa tacactgatg ccccctccca ggagcaggtt cgtgtaaaaa ctatgcttaa
      241 tagcaccatt tcgatgggtt acccggatgt tgtaattgca tgtatagaac atcaagtatc
      301 tctggaagca ttcagggcaa ttgaggccgc gttagtaaag cacgataaga atacgaagga
      361 ttattccctg gtggttgact gatcaccata actgctaacc attctgacca tttcacaagt
      421 gacagagtca atatcgtatt ctgtcactgt caggctaata cagtactgca attcaactac
      481 agcaatgcct cgtatttagg tgaatttaca atatcgtcca gttcggatgc cggctgcatt
      541 tctgaagata aggcatttcg gttcgcgtat tttcccctca tgctcgtcag tcctgcgcgt
      601 agtaagaaac aggacactca cactaatttg tgtgggcatg ctgtgatgtc cttctgaatt
      661 atccccacgc cattatgtaa agctctgttt cagatgctcg tcacggttgt caggctgtcg
      721 ggtcctccct gtggggggcc ctgccacggg gcgggagcgt cgcggaaaaa ggctagtttt
      781 tgaaatttca ttcgtcatca ccaccactgt aatagattga tattacagtg gttttatttt
      841 tatggtgtcg attttgattg ttttttgttc atcactaaca ccgtttgcct aaagttgttc
      901 gcaagatgca tgtttaaaac attctggagc gggtatggat cgagagttaa aaaatctgac
      961 gctgaatatc agtcaactgg cggcactgtc aggtgtacat cgccagactg ctgcggcaag
     1021 gctgcaaaat ctacccgttg caggggggca tgaaagcaac ctcaagcttt atcgggtggt
     1081 tgatattgtg tcggcatttc tggcattacc accgccggtt gcagaaggcg aaatggacgc
     1141 gcatgagcgc aaagcctggt atcagtctga acgtgagcgt cttaagttcg aacaggaaac
     1201 ggcacaactc attccggcca gtgatgtcag acgggagttt gccatctggg caaaagcggt
     1261 cgtgcaggtg ctggagacat taccggatat tcttgaacgt gactgcggtc tgcagcctgc
     1321 cgctgtgagc cgtgttcagt ccattattga tgatctgcgc gatcagatag ccctgcgggt
     1381 gactgaagca ggtgcggatg atgaggagga attacagcag gaggagtaat gctgaatcag
     1441 gaaaccgcaa aggcagcacg aaccgattca ggttatatcc ttcgcgcacc gagacgaatg
     1501 cgggttgctg atgccgttgc tcagtatatg cgggtgccca tgggggcagg gaactcagtc
     1561 ccgtgggatc cgctggtggc accgtatgtt attgagccga tgaactgcct ggcctcgcgt
     1621 gaatacgacg cagtgatatt tgttggcccg gcacgaaccg gcaagactat cggcctgatt
     1681 gacggctggg tgatttacaa cgtgatttgc gatcctgctg atatgctgat cattcagatg
     1741 acggaggaaa aagcccgcga acactccaaa aaacgactcg ccagaacgtt tcgcgtcagc
     1801 ccggaagtgg tcagtcgcct gagtccgaac aaaaatgaca acaacgttta tgacagaaca
     1861 ttccttgctg gtaactacct gaaaatcggc tggccgtcag tcaatatcat gtcctcatca
     1921 gattataaat gcgtcgcgct gacggattat gaccgttttc cggaagatat tgatggcgag
     1981 ggggatgctt tctctcttgc ctcaaaacgt accacaacat ttatgtccag tggtatgacg
     2041 cttgtggaga gttcccccgg cagggatgtg aaggatgtga aatggcgacg gacttcaccg
     2101 catgaggctc caccaaccac ggggatactg tcgctctata accgtggcga tcgccgtcgc
     2161 tggtactggc cctgtccaca ctgtggtgag tattttcagc cctgcggcga tgtggttgct
     2221 ggtttccgtg atattgccga tcccgtgctg gcaagtgagg cggcttatat tcagtgtcct
     2281 tcctgttcag gacggattat gcctgaacaa aaacgtgagc tgaacggacg tggggtctgg
     2341 ttgcgggatg gtgaatccat caatgcggat ggcagtcgtt atggtgatcc ccgacgctca
     2401 cgtattgcgt cattctggat ggagggtccg gcagctgctt accagacact ctcgcaactc
     2461 gtttacaaac tgcttactgc agaacaggaa tacgagacaa ccggaagtga agaaacactc
     2521 aagacggtta tcaataccga ctggggatta ccttatcttc cccgcgccag catggagcaa
     2581 cgaaaaagtg aactgcttga gcagcgggca gagccagttc cttcccacag tgtgccggat
     2641 ggcgttaatt tccttgtggc gacagtggat gtgcaggcgg gacgtcatcg ccgttttgtg
     2701 gttcaggtaa cgggctatgg cagccgtggc gaacgctgga ttattgatcg ttacaacatc
     2761 acgcagtcat tgcgcggtga cagcgacggg gagagccagc gaattgatcc ggccagctat
     2821 ccggaagact gggatgtcct gctgacggat gtttttcata aaagctggcc gctggcctcc
     2881 gatccttctc aacaaatgcg actgatggca atggcggtgg actccggcgg tgaagacggg
     2941 gtcactgata atgcctataa attctggcgt cgttgccgtc gtgatggcct tggtaaacgt
     3001 atttacctgt ttaagggcga cagcatccgg cgcgcaaaac tgatcactcg tacattccct
     3061 gataacaccg gacgaacggg ccgacgggcg caggccgcag gtgatgtgcc gctctggctt
     3121 cttcagacgg atgcactgaa agaccgggtg aataacgcgt tatggcgtga ctcgccaggt
     3181 cccggctatg tgcatttccc tgactggctg gggagctggt tttacgacga actgacgtat
     3241 gaagagcgga gcagtgacgg gaaatggagt aagcctggtc gcggtgccaa cgaagctttt
     3301 gacctgatgg tgtatgccga ggctctggtc attctgcatg gatacgaaaa gatccgctgg
     3361 ccggatgcac cggagtgggc gagccgggaa acctggctgg agtgtgtcca ggacagtacc
     3421 gaaccgtcat cctcaccgga accggtatcc acgcctgtta aaaaacaaaa acggaagaaa
     3481 acagtaactg acgatgttaa cccctggctg acttccggag gatggttatg aaccagaatg
     3541 atatcgaagc catgattcag cgttatatgg aagctgaaat ggcggtgctg gacggaaaat
     3601 ccgtcacctt taatggtcag cagatgacca tggaaaactt atctgagatc cggcagggac
     3661 ggcaggagtg ggagcgccgc cttgcggctc tgattacacg acgacggggg catcccgggt
     3721 accggctggc gaggttctga tggcaattct tgatgatgtg attggcgttt tttcacctgg
     3781 atggaaagcg gcaaggctgc gttcccgtgc ggtgatccag gcttatgaag ccgtaaaaac
     3841 gacgcggaca cacaaagccc gacgggagaa ccgaactgcc gaccagctaa gccagtacgg
     3901 ggccgtgtcg ttacgtgagc aggcccgtta ccttgataac aaccacgatc tggttattgg
     3961 tgtatttgac aagctggaag aacgggtggt ggggaaaaac gggattattg tcgagccaca
     4021 tccggtatta cgcaatgggg ccattgcccg tgatctggca gcggagattc gcacccgatg
     4081 gagtgaatgg tctgtcagcc cggaagtcac cgggcagttt acccgtccga tgctggaacg
     4141 tctgatgctg cgtacctggc tgcgcgatgg tgaggtgttt gtccagatgg tttccgggcg
     4201 cataaacagc ctgacgcctt ctgccggtgt tcatttctgg ctggaggcgc tcgagccaga
     4261 ctttattccc atgaccagtg atgagagcaa caggctgaat cagggcgtgt ttgttgatga
     4321 ctgggggcgt cccgaaaaat atctggtgta taaaagccgt cccgtatccg gacggcagat
     4381 ggaaaccaaa gaagtggatg cagagcgaat gctgcatctt aaatttgttc gccgtctgca
     4441 ccagatgcgc gggacgtctt tgttgtccgg tgtgctgatc cgcctcagtg ccctgaaaga
     4501 ttatgaagat tatgagctga ctgcagcaag gatcgccgct gctctgggga tgtacatccg
     4561 caaaggcgac gggcagagct atgaaccgga tggtaatggc agcaaggata aggaacgcga
     4621 gcttaccatt cagccaggca ttatttacga cgatctgaaa cccggcgaag aaatcggaat
     4681 ggtgaagtcg gatcgcccca atcctaacct tgaaactttt cgtaatggtc agttgcgtgc
     4741 cgtggcggcg ggcagtcgtc tgagtttttc cagtacagcg cgcaactata acggcactta
     4801 cagcgcccag cgtcaggagc tggttgaatc tactgatggc tacctgatcc tgcaggactg
     4861 gtttattggt gccgtcaccc gcccgatgta tcgtgcatgg ctgaaacagg ctgtggcatc
     4921 cggtgttatc aggctacccc gcgatcttga ccgttcttca ctgtataccg cggtgtattc
     4981 cggaccagtg atgccgtgga ttgaccctgt taaggaggct gaggcctgga aaatccagat
     5041 tcgtggtgga gcggcgacag aatcagactg ggtacgtgct ggtggtcgta atccggatga
     5101 tgtcaaacgt cggcgcaagg ccgaaattga tgaaaaccgc aagctggatc tggtatttga
     5161 taccgatccg gccagtgata aaggaggcag cagtgccgca acgaaacgac aggagccgca
     5221 gcacaccgac gaccagtccg aagaataatt cctggttcag gatgcaggct ggtcaccaga
     5281 gtgacgcgga tatttatatt tatgacgaga ttggtttctg gggtgttaca gcgaagcagt
     5341 ttatcagtga tctgaatgca ctgggcgata tcacccacat taatctccat attaattcac
     5401 cgggtggcga tgtctttgaa ggcatcgcca tttttaatgc gctgaaaaca catggtgcgt
     5461 ccattaccgt ttatgtcgac ggtgtggcgg cgtcaatggc gtcggtcatt gcgatggtgg
     5521 gaaacccggt cattatgccg gaaaacacct tcatgatgat tcataaacca tttggcttta
     5581 cgggcggtga tgcggaggac atgcgcacct atgccgacct gctcgataag gttgaggcgg
     5641 ttctgttacc cgcttatgca cagaaaaccg ggaaaaccac cgatgaaatt gctgccatgc
     5701 tggcggatga gacctggatg tccggtgccg aatgtctggc acagggattt gctgatcagg
     5761 tgacgccagc cgttaaggca atggcatgta ttcagtcaaa acgtacagag gaatttaaaa
     5821 agatgccgga atccattcga aacatgatta ctccgccacg caacagtgct ccacgcgtac
     5881 aggatgatga acctgcagcc tcccggacgc cagtgcaggc agcagcaccc gtggtggatg
     5941 aaaacagtat ccgtgcgcag gtactggcag agcaaaaagc gcgtgtaaac ggtattaatg
     6001 atctgtttgc catgtttggc gggcgttatc agacgctgca ggctcagtgt cttgccgatc
     6061 ctgaatgttc gctggagcag gcccgcgaaa agctgttgaa cgagatgggg cgcgagtcca
     6121 cgccatccaa taaaaatacc ccggctcata tttatgccgg aaacggtaat tttgtggggg
     6181 acgggatccg ccaggcgctg atggcgcgtg ccggatttga aaaaaccgaa cgtgataatg
     6241 tctacaacgg gatgaccctg cgtgaatatg cccgtatgtc actgactgaa cggggtattg
     6301 gggtttccag ttataacccg atgcagatgg tcggtgcggc gttcacacac agtacgtctg
     6361 acttcggtaa tattctgctg gatgttgcga acaaagccat tctgcagggc tgggaagatg
     6421 cccctgaaac ctatgaacag tggacgcgga aaggtcagtt gtctgatttt aaaattgccc
     6481 atcgtgtggg tatggggggc ttcagtgctc tgcgtcaggt gcgtgaaggg gcggaatata
     6541 aatacgtcac caccggagat aaacaggcca ctattgcact ggcgacctat ggcgagctgt
     6601 tcagtatcac ccgtcaggcc attatcaatg atgatctgaa tatgctgacc gatgtcccga
     6661 tgaaactggg ccgtgcggcg aaatccacta ttgccgatct ggtttatgcc attctgacgt
     6721 ctaacccgaa aatctccaca gataatgtaa gtctgttcga taaagcgaaa catgcaaacg
     6781 tactggagag cgctgcaatg gacgtggcat cgctggataa agcccgccag ttgatgcgcg
     6841 ttcagaaaga gggggagcgt catctgaata ttcgtcctgc gttcgtactg gtaccgacgg
     6901 cgatggagtc tgttgctaac caggtcattc gctcctcaag tgtcaagggg gctgacatta
     6961 acgccggtat tattaacccg gtgaaagatt ttgcgaccgt tattgcagag cctcgtcttg
     7021 atgataacag ccagaccacc ttctacctgg ctgcgtcaaa aggctccgat acgattgaag
     7081 tggcttatct caacggtgtg gatacgccat atattgatca gatggagggc ttcagtgtgg
     7141 atggcgtgac aacgaaagtg cgtattgacg ccggtgtcgc gccagttgat caccgcggtc
     7201 tggtgaaatg tacggcgtaa acgtcgcaga caacaactct gatggcccgt aagggctttt
     7261 tttgtacctg aaatcagccc ctgaacgggg ctgtgcggag acagttatgg caaagaattt
     7321 tgtagaagaa ggaaaaacgg tggcgattgt tgccagtgca gccatcagca gcggagacct
     7381 ggtgcaggtg ggtgatgttt ttgcggtggc gctgaccgat attccacagg gtgaaacagg
     7441 cgacggcctg accgaaggtg tgtttatgct gcctaagctg aaaacggatg acatgaaaac
     7501 gggtaagaag gtttatctga agtccggaaa agttcagttg actaacagcg gctctgatcc
     7561 gctggtcggg gttgtctggg cagatgccgg aaccagtgca gaagaagtgc cggtaaaact
     7621 caatgtctga tcccttttcc cggctggcag cgcgtatgga tgcgatcacg gtcagaaaga
     7681 tgggaaagac agcctcgatt aatgatgtcg atatgactgt gatcccggga gaaacactgg
     7741 cagagctgaa tgctctgtcc ggacctgcgg tctctctggt ggtgttttct tcgggatacc
     7801 gcccacggcg cggggatcgc gttgtttatg acggacaaca ttggacggtc acacggcatg
     7861 aacgctttaa cggtaagcca atgatcttta ttgagtaaag aggtgtggga tgaaggggct
     7921 tgagaatgcc atccgcaatc tgaacagcct tgatacccgt atggtgccac aggccagcgc
     7981 atgggcgata aaccgtgtgg cacagaaagc ggtctcggtt gccacccggc aggttgccgg
     8041 gaataccgtt gcgggagata accaggtgaa agggataccc ctgaaactgg tacgtcagcg
     8101 tgtccgggtg tttaaagcca gtccgtcagg aaaaatgacg gccaggatcc gcgttaaccg
     8161 gggcaatctg cctgccatta agctggggac cgcccgggtc agactgaccc ggcgtggtgg
     8221 aaaactgcag taccgtggca gcgtgctgaa ggtgggtaaa tatcttttcc gggatgcgtt
     8281 tattcagcaa ctggtgaatg gtcgctggca tgtgatgcgg cgtattgatg gcaaaaatcg
     8341 ttaccccatt gatgtggtga aaatccctct gtccggaccg ctgacacagg catttgaaga
     8401 tgcccgcgac cgcatcattg ctgcggaaat gccgaaacag ctggggtatg cactgaaaca
     8461 acaactgagg ttatggctga cccgatgaac cgacatacac aaatccgcca ggtcgtactg
     8521 gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacggact tccggcattt
     8581 gttgatgcgc aggaactgcc tgccgtggcg gtgtggctga gtgatgctca gtacaccgga
     8641 aaaatgacgg atgaagatga ctggcaggct gttctgcata ttgctgtctt catccgggca
     8701 caggcaccgg attcagagct ggatatgtgg atggagagca ccattttccc ggccctgaat
     8761 gatgtaccgg cactttccgg actcatcgac accctgatcc cactcggttt taactatcaa
     8821 cgtgataatg agatggccac ctgggcgatg gcggaaatca cgtaccagat cacgtacacg
     8881 aattaaggag gtggtaatga ccacaccaaa tccactggca aaaacgaaag gtgcgggaac
     8941 gacgttctgg atgtacaccg gcaagggcga tgcgtttgcg aaccctttat cggacactga
     9001 ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg ctgatgcaga
     9061 agatgacact tatctcgatg atgaagatgc agactggaaa acgacaactc aggggcagaa
     9121 atccgtcggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg gacagaaaaa
     9181 actggttcag ttgttcgact ccggtgaaat ctgcgcgttt cgtatcaaat atcccaacgg
     9241 cactgttgat gttttccgtg gctggttgag ttcactgggt aaaaccattg cctcaaaaga
     9301 cgtgatgacc cgcaccgtga aaatcagcgg tgtggggcgt ccgtatctgg cagaggaggg
     9361 ctctgaaacc gtgggcgtta ccgggctgac ggtggcaccg gcatccgcca gtatcaatgt
     9421 gggagcaacc accacgctga cctttacagt aaaacctgac ggagccagtg acaaagcgat
     9481 cagtgtgcat tcgacagatc cacagacagc cactgtgacc ctgaacggac ttgtggccac
     9541 ggtgaaaggc gtgaagcagg gcagtgtcag cattgtgggc atgacttctg acggcgattt
     9601 tgtggcagtg gctacggtgg ctgtcagcgc cgcaggttaa caggacgata ctcatcattt
     9661 gccccggtta ttcggggctt ttttgcaggt ggagaatatg atgtttctga aacagggcac
     9721 gtttaattat gaaaaacagt ccgtggtgct cagtgagctg tccgggctgc agagaattga
     9781 atatctggcg tttgttcagc agcgaacggc aaagtttgat gccggggagg gagaactgtc
     9841 ggaggctgaa cgacagattg cttttctgcg gatggggatg gatatcaatg cctggctggt
     9901 ttcccgctca ctgtggaatg cggatcagtc taaggatgta gagacgcttt gcgcatccgt
     9961 tattacaaca tggtcgtatg atgccctggg ggcgggggcg gagatggttc tgtcgctgag
    10021 cggtatgggg gccattgata atgccgggga tttggagcat gaggcgctga cgccggaaaa
    10081 gtcctgacgc gggaaatgca gtttgtcatg cggcttgccc gggagttccg gcgggcagac
    10141 tggcggcgga tgctgtcgga aatgtcggcc actgagcttg gtgagtgggg cgattatttc
    10201 cggatgcaga gcttcagtga tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg
    10261 atcgtgagaa tggtgtccgg cagcagtgat gctgcggtgg ctgatttcag ccttttaccg
    10321 gaagagaacg ggataccgga gcgaacggac gaagaactga tgcatcttgg ggaaggtatt
    10381 tccggaggtg tgcgttatgg accagatagc caacctggtc attgatttgg ggattgatgc
    10441 ggcagagttt aaaaatgaaa ttccccgtat caaaaacctt ctgaatggtg ccgccagcga
    10501 tgcagaacgg tcttctgccc gtatgcagcg ttttatggag cgtcagactc aggccgcccg
    10561 gcagacaacg caggcggctt cttcggctgc aacagccgcc tccgtccatg cgcagacggt
    10621 ggagaagagc gcacaggctc atgaacgcat ggcccgcgag gtggagaaaa cccgccagcg
    10681 catggaggca ctgagccaga aaatgcgcga tgaacaggcg caggccatgg ctctggcgga
    10741 ggctcaggat aaagcggctg ctgcgtttta tcgtcagatt gacagtgtga aacaggccag
    10801 tgcggggctg caggaattac agcgtattca gcagcagatc cgacaggcca gaaacagtgg
    10861 cgggattggt cagcaggatt atctggcgct gatttctgag gttacggcga aaacccgtgt
    10921 tcttacacag gctgaggaag aggctacccg acagaaagtg gcgtttatcc gtcagcttaa
    10981 agagcaggca acccgccaga atctttcttc ttctgagttg cttcgtgcca aggctgccca
    11041 gctgggggta agcagtgctg cagaagtgta tatccgcaaa atggagcagg caggaaaagc
    11101 cacgcattcg ctgggtctga aaagtgcagc ggcccgccag gagataggcg ttctgatagg
    11161 tgaactggct cgcggcaatt taggtgcgct gaggggatcc gggataacgc tggctaaccg
    11221 tgccggatgg atagacacac tgatgtcacc gaaaggcatg atgctgggcg gggttattgg
    11281 cggtattgcc gcggccgtct atggtctggg taaagcctgg tatgacggtc agaaggaggg
    11341 ggaagaattt aaccgccagt tgtcgctgac ggggcattat gccggagtca ctgccgggca
    11401 gctgtggacg ctcagtcgtg ctatttccgg gaatggtatc acgcaacatg ctgcagccgg
    11461 tgcgctggct caggtggtgg ggagtggtgc atttcgtgga aacgatatcg gtatggtggc
    11521 gagagctgcc gcacagatgg agcgatcggt tggccagtcg gtcagcgata ccataaatca
    11581 gtttaagcgg ctgaaggatg atcctgtaaa tgccgcgaag gctctggaca atgagctgca
    11641 ttttcttact gccactcagc ttgagcagat acgtgtcctt ggggatcagg gacggtccag
    11701 tgatgctgca cggatagcca tgtctgcact ggcagaggaa accggtcggc gtactgcgga
    11761 tattgataat aacctcaatg cgctgggcag tacgctgaag tatctgtctg atttatggag
    11821 tcgtttctgg gatgcggcca tgaatattgg tcgtgaagac tcgctggatg aacagatttc
    11881 cgctttacag gagaaagtgt cgcgggcgaa aagactcccc tggacggcat catcttctca
    11941 ggttgaatac gatcagcagc gtcttaacga gcttcaggag aaaaaacgcc agaaggattt
    12001 gcaggatgca aaagagcagg cagagcggaa ttatcaggag caacagaaac gccgtaatgc
    12061 tgaaaatgct gcactgaacc ggatgaatga aacggaagca gcacgacatc agcgtgaaat
    12121 tgcgcgtatt aatgccatgc agtacgccga tcaggctgtc agggatgcgg cgatacaacg
    12181 tgaaaatgaa cgttacgaga aagccctggc atccggtaag aaaaaaacac gcgaaacccg
    12241 taatgatgag gccacccggt tattgctgca gtacagtcag caacaggcac aggtggaagg
    12301 acagattgct gctgccagac agtcatcagg cattgccacg gaaaggatga cagaagctca
    12361 taaacagctt ctggctctgc agcagcgcat cagcgacctg gacgggaaaa aactgacggc
    12421 agatgaaaag agtgtgctgg cccgtaaaga tgaactgatt caggcactga cgctgctgga
    12481 tgtaaaacag caggagcttc agaaacagac ggcactcaac gagctgaaga aaaaaacaat
    12541 tcagctgacc agtcaactgg ctgaagaaga gcgcgctcag cgtcagcaac atgacctgga
    12601 tattgccacg gtgggtatgg gtgatcagca gcggcagcga tatcaggtac aactgagtct
    12661 tcgccagaaa taccagcaac agctggagca gttgaggcgg gatagtgagc agaaaggaac
    12721 atataacacg gatgactaca gaaaggccga gcaggcgctg acggagagcc tgaaccgaca
    12781 actgaatgag aatcgccgtt actggcaaca gcttgaagtt gtgcagggta actggaaaaa
    12841 cggagtcctg cgtgcatttc aggattttac cgtggatgca gataatacgg caggaacagc
    12901 agaacaggtg ttctcgtcag ccttcagcta catgggaaat ggcctggcaa cttttgtcac
    12961 taccggcaaa ctcaatttca aatccttcac ctcttctgtg ctgtcagata tggcgaaaat
    13021 cctggcgcag gcaaccatga tgaaggctgt caaagggatt ggtagtgcac tggggatggg
    13081 ttctgttctc ggcagccttt cccttaatgc tgatggtggt gtttatcact ctgcagattt
    13141 gagtcgctac agtggttctg tggttaaccg tccgacgttt tttgcttttg caaaaggcgc
    13201 gggtgtgatg ggggaagcgg ggcctgaagc cattctgcca ctgcgtcgtg gtgccgatgg
    13261 taagctgggt gttgtggcgg atattggtgg ttcaggtatc gcgatgtttg ccccgcagta
    13321 caacattgag atcaataacg acggcacgaa cgggcagata ggtccggctg ccctgaaggt
    13381 ggtttatgac ctcgggaaaa aagcggcagc ggactttatg caacagcagg cccgcgatgg
    13441 tggtcagtta agtggagcat atcggtaatg gagacgtttc actggaaagt acgtccggat
    13501 atgaatgtcg tatcagagcc aaaggtagtg acagtgaagc ttggcgatgg ttatgaacaa
    13561 cgtcgtgcgg cggggctgaa taaccagctg tcgacttaca gcgtgacgat acgtgttcgt
    13621 aaatgtgaac accagtcttt aaaagccttt ctggaacagc acggtggcgt ccgtgcattt
    13681 cagtggacgc caccttatga ctggaaaccg atcagggtgg tttgtcgtaa atggtcggca
    13741 agcgtggggg cgctgtgggt aaccataacg gcagattttg aacaggtcgt ggcataggag
    13801 actctgatgc aggatattcc actggaaaca catcatgaga cgacacgcct cactcagtca
    13861 gcccaggtgg tgctctggga aattgacctg acagaggtcg gtggagaacg ttattttttc
    13921 tgtaatgagc agaacgaaaa aggtgagccg gtcacctggc aggggcgaca gtatcaggcg
    13981 tatcccattc aggggagcgg ttttgaactg aatggcaaag gcaccagtac gcggcccacg
    14041 ctggcagtct ctaacctgta cggcatggtc accgggatgg tggaagatat gcagagtctg
    14101 gtcggcggaa cggtggtccg gcgtaaggtt tacgcccgtt ttctggatgc ggtgaacttc
    14161 gtcaacggaa acagcgacgc cgatccggag caggaggtga tcagtcgctg gcgcatcgag
    14221 cagtgcagcg aactgagcgc ggtcagtgcc tcttttgtac tgtccacgcc gacggaaacg
    14281 gacggcgctg tttttccggg acgtatcatg ctggccaaca cctgcacctg gagctatcgc
    14341 ggtgatgagt gcggttatca cggtccggcg gtcgcggatg aatatgacca gccgacgtcc
    14401 gatatcacga aggataaatg cagcaaatgc ctgagcggct gtaagtttcg caataacgtc
    14461 ggcaactttg gcggcttcct ttccattaac aaactttcgc agtaatccca tgacagagac
    14521 agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt gcggcttcgt
    14581 ggtgagagcg ccggaggggg aaagatattt
//
LOCUS       DAZKHK010000122        14521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKHK010000122 DAZKHK010000000
VERSION     DAZKHK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.122"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            141..1808
                     /gene="fdrA"
                     /locus_tag="ACHOS5_003949"
     CDS             141..1808
                     /gene="fdrA"
                     /locus_tag="ACHOS5_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9778691.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTSSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            1818..3077
                     /gene="ylbE"
                     /locus_tag="ACHOS5_003950"
     CDS             1818..3077
                     /gene="ylbE"
                     /locus_tag="ACHOS5_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9778692.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     TVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            3088..3903
                     /gene="ylbF"
                     /locus_tag="ACHOS5_003951"
     CDS             3088..3903
                     /gene="ylbF"
                     /locus_tag="ACHOS5_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9778693.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQALAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            3900..4793
                     /gene="ybcF"
                     /locus_tag="ACHOS5_003952"
     CDS             3900..4793
                     /gene="ybcF"
                     /locus_tag="ACHOS5_003952"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9778694.1"
                     /translation="MKTLVVALGGNALLQRGEALTAKNQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDILVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVAEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(5026..6093)
                     /gene="purK"
                     /locus_tag="ACHOS5_003953"
     CDS             complement(5026..6093)
                     /gene="purK"
                     /locus_tag="ACHOS5_003953"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9778695.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARQPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLSE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRAAETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQNGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6090..6599)
                     /gene="purE"
                     /locus_tag="ACHOS5_003954"
     CDS             complement(6090..6599)
                     /gene="purE"
                     /locus_tag="ACHOS5_003954"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9778696.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILANHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(6695..7024)
                     /locus_tag="ACHOS5_003955"
     CDS             complement(6695..7024)
                     /locus_tag="ACHOS5_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778697.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERFILIVGICFFPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            complement(7135..7857)
                     /gene="lpxH"
                     /locus_tag="ACHOS5_003956"
     CDS             complement(7135..7857)
                     /gene="lpxH"
                     /locus_tag="ACHOS5_003956"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP9778698.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(7860..8354)
                     /gene="ppiB"
                     /locus_tag="ACHOS5_003957"
     CDS             complement(7860..8354)
                     /gene="ppiB"
                     /locus_tag="ACHOS5_003957"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP9778699.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNIRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            8528..9913
                     /gene="cysS"
                     /locus_tag="ACHOS5_003958"
     CDS             8528..9913
                     /gene="cysS"
                     /locus_tag="ACHOS5_003958"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9778700.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(9949..10470)
                     /gene="ybcI"
                     /locus_tag="ACHOS5_003959"
     CDS             complement(9949..10470)
                     /gene="ybcI"
                     /locus_tag="ACHOS5_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9778701.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(10578..10790)
                     /gene="ybcJ"
                     /locus_tag="ACHOS5_003960"
     CDS             complement(10578..10790)
                     /gene="ybcJ"
                     /locus_tag="ACHOS5_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP9778702.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(10792..11658)
                     /gene="folD"
                     /locus_tag="ACHOS5_003961"
     CDS             complement(10792..11658)
                     /gene="folD"
                     /locus_tag="ACHOS5_003961"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP9778703.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            11699..11896
                     /locus_tag="ACHOS5_003962"
     CDS             11699..11896
                     /locus_tag="ACHOS5_003962"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778704.1"
                     /translation="MSILSEAWRAVSFRFLKSKTFCTWLIHTYHDNRRWFAAIVSYIR
                     CGRTLVQQLVQKPLTQQGLTV"
     gene            11929..12005
                     /locus_tag="ACHOS5_003963"
     tRNA            11929..12005
                     /locus_tag="ACHOS5_003963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11963..11965,aa:Arg,seq:tct)
     gene            complement(12021..13184)
                     /gene="intD"
                     /locus_tag="ACHOS5_003964"
     CDS             complement(12021..13184)
                     /gene="intD"
                     /locus_tag="ACHOS5_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9778705.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(13316..13495)
                     /locus_tag="ACHOS5_003965"
                     /pseudo
     CDS             complement(13316..13495)
                     /locus_tag="ACHOS5_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(13411..13716)
                     /gene="yfdT"
                     /locus_tag="ACHOS5_003966"
     CDS             complement(13411..13716)
                     /gene="yfdT"
                     /locus_tag="ACHOS5_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFP9778706.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            complement(13716..14078)
                     /locus_tag="ACHOS5_003967"
     CDS             complement(13716..14078)
                     /locus_tag="ACHOS5_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778707.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(14069..>14521)
                     /gene="yfdR"
                     /locus_tag="ACHOS5_003968"
     CDS             complement(14069..>14521)
                     /gene="yfdR"
                     /locus_tag="ACHOS5_003968"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9778708.1"
                     /translation="VSLSNICRFAGHLSHFYSVAQHAVLCSQLVPQEFAFEALMHDAT
                     EAYCQDIPAPLKRLLPDYKRMEEKIDAVIREKYGLPPVMSTPVKYADLIMLATERRDL
                     GLDDGSFWPVLEGIPATEMFNVIPLAPGHAYGMFMERFNDLSELRKCA"
BASE COUNT         3323 a         3782 c         3948 g         3468 t
ORIGIN      
        1 tctggcggtt atttgaattc ttttgttaat aatccacgtg tgatattcat caccttattt
       61 actcgttgtc atcgataccg taatcgccac attaacactg ctcgtgcaat tgccatgggt
      121 gcaattttta aggagttgtt atgatccacg cctttattaa aaaagggtgt tttcaggatt
      181 cggtcagttt aatgattatt tcacgaaaac tcagcgaatc agaaaatgtt gatgatgttt
      241 ccgtaatgat gggtacgccc gccaataaag cgttattaga tactacaggt ttctggcatg
      301 acgattttaa taacgccacg ccgaacgata tttgcgtggc aattcgtagc gaagcggcgg
      361 atgcggggat cgcgcaggcg gttatgcagc agcttgaaga ggcgctaaaa caactggcgc
      421 agggttcagg cagcagccag gcgttgacgc aggtgcgtcg ctgggacagt gccagtcaga
      481 aattacccga tgccaatctg gcgctgattt cagtggctgg tgagtatgcg gcggagctgg
      541 cgaaccaggc gctggatcgc aacctcaacg tgatgatgtt ctccgataac gtcacgctgg
      601 aagatgaaat ccaacttaaa acccgcgcgc gggaaaaagg cttgctggtg atggggccag
      661 actgcggtac gtcgatgatt gccgccacgc cgctggcttt tgctaacgtg atgccggaag
      721 gcaacattgg cgtcataggc gcttccggta ccgggattca ggagctgtgc tcacagattg
      781 cgctggcagg ggagggaatt actcacgcga ttggtcttgg cgggcgcgat ctcagccgtg
      841 aagtgggcgg catcagtgcg ctaacggcgc tggaaatgct cagtgcagac gagaaaagcg
      901 aagtgctggc atttgtttca aaaccacctg ccgaaactgt gcgtctgaaa attgttaatg
      961 ccatgaaagc aactggcaaa ccgacggtgg cgctgttttt aggttatacc ccggcggtgg
     1021 cccgcgacga gaatgtctgg tttgcctcct cgctggatga ggccgctcgt ctggcttgtc
     1081 tgctttcacg cgtcactgcg cgacgtaacg cgatagcgcc tgtcagcagc ggatttattt
     1141 gcggtttgta taccggcggt acgctggctg ctgaagcggc gggattactt gccggacacc
     1201 ttggcgtgga agccgacgat acccatcaac atggcatgat gctggacgcc gacggacatc
     1261 agatcctcga cctgggcgat gatttttaca ccgtcgggcg tccccatccg atgatcgacc
     1321 cgaccttacg caacctgtta attgccgatc tcggcgctaa accgcaagtg cgcgtgttgc
     1381 tgcttgatgt cgtgattggc ttcggtgcga ccgccgatcc tgccgcctcg ctggtgagcg
     1441 cctggcaaaa agcctgtgcc gcgcgttcag acaatcaacc actgtatgcc attgccacgg
     1501 tgacaggcac tgaacgtgac ccgcaatgcc gctcgcagca aatcgccacg ctggaagatg
     1561 cggggatcgc ggtagtgagt tcgctaccgg aagccacctt gctggcagcg gcgttaattc
     1621 atccgctctc gcctgccaca cagcaacaca catcgtcatt actggaaaac gtcgccgtga
     1681 ttaacatcgg attacgcagc tttgcgctgg agctacaaag cgccagcaaa ccggttgtgc
     1741 attaccaatg gtcgccagtc gccggcggca ataaaaaact ggctcgttta ttagaacgtt
     1801 tgcaataagg ggttcccatg tttacatcag tggcgcaagc caatgctgcg gttattgaac
     1861 aaattcgtcg ggctcgtcca cactggctgg atgtgcaacc ggcttcttca cttatcagcg
     1921 aactaaacaa gggcaaaaca ctgcttcacg ccgggccgcc aatgcgctgg caggagatga
     1981 ccgggccaat gaaaggtgca tgtgtgggcg catgtctgtt tgaaggctgg gcgaaagatg
     2041 aagcgcaggc actggcaatg ctggagcagg gggaagtgaa cttcattcct tgtcaccatg
     2101 tgaatgctgt cgggccaatg ggcggcatta cttctgccag tatgccgatg ctggtggtcg
     2161 agaacgtgac cgacggcaac cgggcgtact gcaacctcaa cgaaggtatc ggcaaagtga
     2221 tgcgttttgg cgcttacggc gaagatgtcc tgactcgcca tcgctggatg cgcgatgtgt
     2281 taatgccagt attaagcgcg gcgctggggc gcatggagcg cggcatcgat ctcacggcaa
     2341 tgatggcgca gggcattacc atgggcgatg agttccatca acgcaacatt gcttcctctg
     2401 cactgttaat gcgtgcgctg gccccacaaa ttgctcgcct cgatcatgat aaacagcaca
     2461 ttgcggaagt gatggatttc ctcagcgtga ccgatcagtt cttccttaac ctcgcgatgg
     2521 cttactgcaa ggcggcgatg gatgcaggcg cgatgatccg cgcaggcagc atcgttacgg
     2581 ctatgacccg caacggcaat atgttcggga ttcgggtcag cgggctgggt gaacgctggt
     2641 ttacggcgcc tgtaaacact ccgcaaggtc tgtttttcac cggcttctcg caggagcagg
     2701 cgaacccgga tatgggcgac agcgcgatta ccgaaacctt tggtattggc ggagcggcga
     2761 tgatcgcagc gcctggcgta acgcgctttg tcggtgcggg tggcatggaa gcggccagaa
     2821 ccgtttctga agagatggcg gaaatttacc ttgagcgcaa tatgcagttg cagatcccaa
     2881 gctgggattt tcagggcgcg tgcctggggc tggatattcg tcgcgtggta gaaaccggca
     2941 ttacgccact catcaatacc ggtatcgccc ataaagaggc ggggatcggg cagattggtg
     3001 caggcactgt gcgggcaccg ctggcgtgct ttgaacaggc gctggaagca ctggctgaaa
     3061 gcatggggat tggttgagga acgcacaatg acgatcatcc atcctctact tgccagccgt
     3121 agcgcaccga attatcgcca gtcctggcgg ttagcgggag tgtggcggcg ggcgattaac
     3181 ctgatgacgg aaagcggcga actgttaacg ctgcatcgtc aggggagtgg tttcggcccc
     3241 ggcggatggg tgcttcgccg cgcgcaattc gatgcgttat gcggtggatt atgcggcaat
     3301 gaacgaccac aggttgtggc acaagggatt cgcctcgggc gtttcacggt taaacagcca
     3361 cagcgttatt gtttgctacg tattacgccg cctgcgcatc ctcaagcgct tgccgttgca
     3421 tggatgcaac gcgcggagga aaccgggctt ttcgggccac tggcattggc ggcaagcgat
     3481 ccgctgcctg ctgaattacg ccagtttcgt cactgttttc aggccgcgct caatggcgtt
     3541 aagaccgact ggcggcactg gctgggtaaa ggccccggat taacgccaag tcatgatgac
     3601 acgctgagcg gaatgctgct ggcggcctgg tattatgggg ctttagatgc gcgctccggt
     3661 cgcccgtttt ttgcctgttc cgacaatctt caactcgtta ccactgcggt gagcgtcagt
     3721 tatttacgtt atgccgcgca aggatatttc gcctcgccac tcctgcactt tgttcatgct
     3781 ctgagttgcc cgaaacgtac cgcagcggcg atcgattcgc tgctggcgct ggggcatacg
     3841 tcaggggccg atacgctgct ggggttctgg cttggccaac aattattaca aggaaaacca
     3901 tgaaaacact ggttgtggct cttgggggca acgccttact ccagcgcggt gaggcgctga
     3961 cggcaaaaaa tcaatatcgc aatattgcca gtgctgtacc cgcgctggcg cgcctggccc
     4021 gttcttatcg gctggcgatt gttcacggca acggaccgca ggtggggctg ctggcgttac
     4081 agaatctggc gtggaaagag gtagaaccgt atccgctgga tatactggtg gcggaaagcc
     4141 aggggatgat tggctatatg ctggcgcaga gcttgagcgc acagccgcag atgccgcctg
     4201 tcacgacggt gctgacgcgc attgaggttt cgcctgatga tccggcgttt ttgcagccag
     4261 agaagtttat tggtccggtt tatcagccag aagaacaaga ggcactggaa gcggcttacg
     4321 gctggcagat gaaacgtgat ggtaaatatt tacgccgggt ggtggcgtct ccgcaaccgc
     4381 gtaaaattct cgacagcgaa gccatcgagt tgttgctcaa agaggggcat gtggtgattt
     4441 gcagtggcgg cggcggtgtg cctgtggcgg aagacggggc tgggagtgaa gcggtgattg
     4501 ataaagatct cgccgctgcg ttgctcgccg agcagattaa tgccgatgga ctggtgatcc
     4561 tcaccgatgc tgatgcggta tatgaaaact ggggaacgcc gcagcaacgt gccattcgcc
     4621 atgccacacc ggatgagtta gcgccatttg ccaaagccga tggttcgatg gggccgaagg
     4681 taacggcggt gagtggctat gtcagaagcc gtggtaaacc cgcgtggatt ggggcgttat
     4741 cgcggattga agagacgctg gcgggcgaag cggggacttg tatttcgctg tagtgatggg
     4801 caccaggcaa ttggcacatc tagcctgatg cgatgctggc gcgtcttatc aggtctacag
     4861 gcggtgtagg aacggtaggc cggataaggc gttcacgccg cgtccggcaa tcggcacacc
     4921 atgcctgatg cgatgctggc gcgtcttatc aggtctacag gcggtgtcgg aacggtaggc
     4981 cggataaggc gtttacgccg catgcggcaa gaaaatgagc atcaattaac tgaacttgct
     5041 ctgcgcccac atcacgccgc tggcatactc cggcggcagc agtgggatta aggcttccag
     5101 cgtcgcggtc agacgcgatg tgtcgctgtc ggtcaaattt aaatgcccca ctttacggcc
     5161 cggacggact tctttgtcgt accagtgcag atgcacaagc ggcagtttca gccagtcata
     5221 attcagatcg ctaccaatca gattgatcat caccgacgga ttattcacta ctggttgcgg
     5281 taacggcaga tcggtaatcg cccgcagatg tagctcaaac tggctgatgc tggcaccgtt
     5341 ttgtgtccag tgaccgctgt tatgcacacg cggagccagt tcgttgatca gcagaccttg
     5401 cggcgtgaca aaacactcca tcgccatcac gcccacatag cccagctcct gcataatcgc
     5461 cgacagcatc tcttcggctt gcgcctgttg ctgtgcattg gcctgcggga aagcgacgct
     5521 ggtgcgcaaa atgccgttct gatgcaggtt atgcgtcagc ggataaaaca cggtgctgcc
     5581 atcaaaaccg cgcgcgccaa ccagcgacac ttcaccagag aagtttatgc cctgctcgac
     5641 aatacattcg ccgtagcatt cagcaggtaa ctgttcggtt tcagcggcgc gtaaacgcca
     5701 ttgaccgcgt ccgtcatagc caccagtgcg gcgcttaaca atcgccagct cacctaaacg
     5761 atcaaacacc gcaggccact cgctgcgttc ggaaagtaac tgccacggtg cggtcggcag
     5821 gtggagctta tcgaaaagct gcttctgagt cagacggtcg gcaataatcg ggaacacatc
     5881 gcggttcaca aaggccggct ggcgcgccag ctcgcgggtt aatgcagttt ccggccagcg
     5941 ttcgatctca gcggtaatca cgctttgttg aaaaggcacc gccgccggtt cagcgtccag
     6001 cccgaccggc cagacagcaa tgcctaacgg ttcgcctgcc tgacgcagca tacggcctaa
     6061 ctgcccgtta ccgaggacgc aaacctgttt catgccgcac ctcgcgggtc cgggttttcc
     6121 agcacttcat cagtttgtgc tttgcgccag tcattcagac gctggtgcag ttctttatca
     6181 tgattcgcaa gaatttgtgc tgccagtaac gccgcgtttg ccgcgccagc tttaccaatc
     6241 gctagcgtac ccaccggaat gccgcgcggc atttgtacga tggagtagag gctatcgaca
     6301 ccgctcagtg cggcgctctg tactggcacg cccagcaccg gcaccagcgt tttggcggca
     6361 atcatgccag gcaggtgcgc tgcgccgcct gcgcccgcaa taatcacctg ataaccgttc
     6421 tcttcggcgc tttcggcgaa gctgaacagt ttatcggggg tgcggtgagc agaaaccact
     6481 tcaacgtggt gcgggacatt caggatttcg aagatttcgg cggcgaactg catggtagcc
     6541 cagtcgcttt tggaccccat cacgatggcg acacgcgccg gattattgcg ggaagacatg
     6601 cgtcttaaaa ctcctgtggt gcacaactct cggctttaga gggcacagag aatagcacgg
     6661 aaagagtgca aggaaaacgg ttgcgtaggc ggaattaacc gtcagttgtt gagtttttaa
     6721 tttgttctgc gtgttgatta tgcgctctgg ctgaactggc aaagaatgcc catatcaaca
     6781 caatgctcca tggaaaaaag caaataccga caatcaatat aaatctttct acaaaacgtt
     6841 ggttatgggg gcgggtgagt actaaataaa tgagccagaa atagactatc caggaaatga
     6901 ccgccgcagc taccagtgac aatgcgccaa aatagaagcc gacaagaccg aaccagctaa
     6961 tacacagtgg cgctcctgcc gtaagggcga tgccagaaat caaaaatccg ttgcagcgtt
     7021 gcataataat attccctgtt gttatttcac tcagagatta ttaccttgct gattttaaaa
     7081 aagatataca ggttgcgtta gttcggtgct ttttcagaat ctgtccattc gtggtcagaa
     7141 aggaaaatga atcagctcaa cgtcatccgc cgtgactttc accatcgaac cttctgtatg
     7201 ccaggcaccc agtaccacgc gaaaagcagg ttgctgattg gcgataagtt catgcaccgc
     7261 cgggcgatgg gtatgcccgt ggatcagcca ttgcacttga tgtttttcca tcgcactgac
     7321 caccgcgttt tggttaacgt ccatgatcgc cagcgattta ctgctgttgg cttctttgct
     7381 gttcgcgcgc attcgcgcgg caatgcgttt gcgcacaaac aacggcaggg cgagaaatag
     7441 cgtttgcagc cagggtttgt ggactttggc gcgaaaagcc tgataacccg cgtcatcggt
     7501 gcacagcgtg tcgccatgca taatcaacac ccgacgacca taaagttcga gcaccttttc
     7561 ttccggcaat aaagtcatgc cgctttcacg ggcaaagcgt ttgccgagca gaaaatcacg
     7621 gttgccatga atgaaataac agggaacgcc ggaatcggat accgctttga tcgctgcggc
     7681 catctggtga tgaagtgggt tgggatcgtc gtcgccgatc caggcttcaa acagatcgcc
     7741 aagaatatac agcgcatcgg ccttgcgggc ctcccccgct aaaaaacgca gaaaaccggc
     7801 ggtgatcgcc ggttcttcca cgcagagatg aagatctgca ataaagagtg tcgccacgat
     7861 tactcgctaa cggtcacgct ttcaatgata acgtcttctt ttggcacgtc ctggtgcata
     7921 ccgctacgac cggttgccac acctttgatt ttgtctacca cgtccatgcc ttcaaccact
     7981 tcagcaaata cgcagtagcc ccaaccttgc aggctttcgc cagagaagtt caggaagtcg
     8041 ttatcaacca cgttgatgaa gaactgggca gttgcagagt gcggtgcctg agtacgggcc
     8101 attgccagcg taccacggat atttttcaga ccgttgttgg cttcgttttt gatcggttct
     8161 ttggtggctt tttgtttcat gcccggttca aaaccgccgc cctgaatcat aaagccgttg
     8221 ataacacggt ggaaaatggt gttgttgtaa aaaccttcgc ggcagtagtc caggaagttt
     8281 ttaactgttt caggtgcttt atcgtcaaaa gttttgatga caatatcgcc gtgattggtg
     8341 tggaaagtaa ccatttttgc atcctgttcc gtttgattgg tgcttcaacc cggttcgggt
     8401 catatatagg gtggtgttat agcataaccg cacgatcgga tcatctcgca atgtatgctg
     8461 attcgcgcgg gaaatatggg tattatacgc aactcaatta cccacacatg tctaaacgga
     8521 atcttcgatg ctaaaaatct tcaatactct gacacgccaa aaagaggaat ttaagcctat
     8581 tcacgccggg gaagtcggca tgtacgtgtg tggaatcacc gtttacgatc tctgtcatat
     8641 cggtcacggg cgtacctttg ttgcctttga cgtggttgcg cgctatctgc gtttcctcgg
     8701 ctataagctg aagtatgtgc gcaacattac cgatatcgac gacaaaatca tcaaacgcgc
     8761 caatgaaaat ggcgaaagct ttgtggcgct ggtggatcgc atgatcgccg aaatgcacaa
     8821 agattttgat gctttgaaca ttttgcgccc ggatatggag ccgcgcgcga cgcaccatat
     8881 cgccgaaatt attgaactca ctgaacaact gatcgccaaa ggtcacgctt atgtggcgga
     8941 caacggcgac gtgatgttcg acgtcccgac cgatccgaca tatggcgtgc tgtcgcgtca
     9001 ggatctcgat cagctgcagg caggcgcgcg cgttgacgtg gtcgatgaca aacgtaaccc
     9061 gatggacttc gttctgtgga agatgtcgaa agagggcgaa ccgagctggc cgtctccgtg
     9121 gggcgcgggc cgtcctggct ggcacattga gtgttcggca atgaactgca aacagttggg
     9181 taaccacttt gatatccacg gcggcggttc tgacctgatg ttcccgcacc acgagaacga
     9241 aattgcccag tccacctgtg cgcacgatgg ccagtatgtg aattactgga tgcactccgg
     9301 tatggtgatg gttgaccgcg agaagatgtc taaatcgctg ggtaacttct tcaccgtgcg
     9361 tgacgtgctg aaatactacg atgcggaaac tgtgcgttac ttcctgatgt cgggtcacta
     9421 tcgcagccag ctgaactaca gcgaagagaa cctgaaacag gcgcgtgcgg cgctggaacg
     9481 tctctatact gccttgcgcg gcacagacaa aaccgttgcg cctgccggtg gcgaagcgtt
     9541 tgaagcgcgc tttattgaag caatggacga cgatttcaac accccggaag cctattccgt
     9601 gctgtttgat atggcgcgtg aagtgaaccg tctgaaagca gaagatatgg cagcggcgaa
     9661 tgcaatggcg tctcacctgc gtaaactttc tgccgtattg ggcctgctgg agcaagaacc
     9721 ggaagcgttc ctgcaaagcg gtgcgcaggc agacgacagc gaagtggctg agattgaagc
     9781 gttaattcaa cagcgtctgg atgcccgtaa agcgaaagac tgggcggcgg cagatgcggc
     9841 gcgtgaccgc cttaacgaga tggggatcgt gctggaagat ggcccgcaag ggaccacctg
     9901 gcgtcgtaag taattgcgct attgccggat gcgagttttc gcatccggtt atcgtctgcg
     9961 ccaccacaac attcccatca gtagcatccc cggcaaccac acccacatca attcagaaat
    10021 aatcacctga tgcccgtacg gcgtggtgta acgagacaat gcaaacggcg cgactttaat
    10081 cacctgccag ggagcgaaaa agcgttcatc tgaccacggc cacagccagc caaccccttt
    10141 accgccagtg gttaccgaat ccagcaagct gtgcgatagc agcgagacgg ttaaaaacag
    10201 ccagcagcga atcagcccag ccctgaacca tcgacgtcca atcagcacac ataacagcgg
    10261 gacaacaaac gcaaacacca gcgaatgggt aaacccgcga tgaccaaaaa cgttgccgta
    10321 agcaacgcca aatttaaacg ataatacgtc ggcatcaggc aacatcgcca ggacgattcc
    10381 ggcaaataac agacgcggtg ggatggcttt cgaacccaac cctaaaccaa tgcatagggg
    10441 aacggcggcg tgcgtaataa cggttggcat gatggtcgct tcggcaaaat gtcaatgcta
    10501 tcagcatgga tgaacggggc gtagagggca aaagtctgaa aagagaaccg gcctgttgat
    10561 acaggccggg aaagggatca ggcaacaacc tgtacgctgt gacctgcaaa actcactgtc
    10621 tgaccggcga caattttgca gcgtttgcgc gtttcaaccg caccgtcgac tttcacctgg
    10681 ccttcggcaa tcgcgatttt cgcctgcgcg ccgctttcgc tccagccttc cagtttcagc
    10741 aagtcgcaca gctcaacgtg cggatgttta cctaaagaaa atgtcgccat gttactcacc
    10801 ctgtggatca tgatattcaa cgcacgcctg tagcgtgttt tcaatcagcg tggcaaccgt
    10861 catcgggcca acgccgcccg gaacaggcgt aatgtatgag gcgcgtttag ccgcgtcttc
    10921 aaacaccacg tcgcccacaa ctttgccatt ttccagacgg ttgatgccga catcaatcac
    10981 aatcgcgcct tctttgatcc agtcaccagg aataaagcct ggcttaccga cggcaacgat
    11041 caatagatcg gcattttcta cgtgatgacg cagattttta gtgaagcggt gagtcacggt
    11101 ggtggtgcaa cctgcgagca atagttccat gctcatcggg cgaccgacga tattcgatgc
    11161 gccaatcacc acggcgttaa ggccgaaggt atcaatgttg taacgctcaa gcagcgtgac
    11221 gataccacgc ggggtgcagg gacgcagacg cggcgcgcgc tggcacaggc gaccgacgtt
    11281 ataaggatgg aagccgtcta cgtctttgtc cggatgaata cgttccagta ctttgacgtt
    11341 atcaataccc gccggtaacg gcagttgaac cagaatgcca tcaatggtgt tgtcggcatt
    11401 cagcgcatcg ataagctcca gcagctccgc ttcgctggtg gtttccggga ggtcataaga
    11461 gcgggagacg aacccgactt cttcacaagc cttgcgtttg cttgcgacat aaatttgcga
    11521 tgcagggttg ctacccacca gcacaacggc cagtcctggc gcccgcagtc cggctgcaat
    11581 acgcgcctga actttttgag caacttcaga gcgcacctgc tgcgcaatcg ttttaccgtc
    11641 aataatcttt gctgccatca gagagaggat tccatctgtt acgtaggtcg aaggggatgt
    11701 gtctattttg tcagaagcgt ggcgcgctgt cagttttcgt tttctgaaaa gtaaaacctt
    11761 ctgtacctgg ctgattcaca cctatcatga taaccgtcgt tggtttgccg ccatagtgag
    11821 ctacatcaga tgcggtcgta cacttgttca gcaactggtt caaaagccat tgactcagca
    11881 agggttgacc gtataattca cgcgattaca gcacaccgtg gtatcaatgc gcccttagct
    11941 cagttggata gagcaacgac cttctaagtc gtgggccgca ggttcgaatc ctgcagggcg
    12001 cgccattaca attcaatcag ttacgccttc tttatatcct ccataattcc acagtgggac
    12061 atatttggga cattatcacc aaaaatgtcg tctattttcc ttgcatgctc tgtcaaatga
    12121 ttaggcgcaa ggtgagcata cctacgaacc atttctatgg actcccatcc gcccatttcc
    12181 tgaagcacag ataatgggac gcctgactgg atcagccagc ttgcccaggt gtgtctgagg
    12241 tcatggaaac ggaaatcttc aattcctgca cgacgacaag ctgatagcca tgatgtcttg
    12301 ctgtcgatgc gcatcttcct gaccgcaggc gttgatgttc catctgctcg cttagccgcc
    12361 ttggtatgta caaacaccca tttgtgatgc ttgcctattt gatcacgcaa cactttacag
    12421 gcggtatcgt tcagcgccac accaatggcg cggtttgatt tgctctcttc tggattcacc
    12481 caggcaactc gtcgctgcat gtcgatttgt tgccattcca gatttatgat gttcgacttt
    12541 ctcagaccag ttgccagcgc aaacttgacg acagatttca gtggttcggg gcactcatca
    12601 ataaggcgtt ttgcttcctc cttttccagc catctgactc gcttgtttct gaccgctggt
    12661 atcttgatga caggcgcttt ttccagccac ttccagtcgc gttctgcagc acggagaatg
    12721 gcctttatca tggcaagatg ctttgccttt gtctgagttg atactggctt tggttcataa
    12781 acaggcggtt ctttaccttt cctgatggcg gcctgaactt tctgtttcca tatttctttc
    12841 gtctttctgt tatgcattct gcttacagca gagtaaatct ttgcctccga gatatcttta
    12901 agccttatac cctcaaaatg ttcaagccag aactcaatcc ggcttttatc tgaatcgaga
    12961 gattttttat cagctttttc ctcaagccat cttaggcagg cctcttcaaa agtgacatca
    13021 ggtaaatccc ctagcttttc tactcgccag agttctgctt ttcgcttgtc gtgcaactcc
    13081 tgagcttgcc gcttgtcctt tgtgccaaga gattccttaa ttcgtttccc gcccgggagc
    13141 gaatacgagg cataccatat ttcatttctg cggaagagtg acattttctt tcctctgtta
    13201 tgccatcacc cgcgctcacc tggacagtat gcagcggaga ctgaagcgcc gcaatgcaag
    13261 cttgccgtgt tgtgaggtaa ggagattttg gcttggttgg atctttacgt gttgcctgta
    13321 ggcggcctgt gcgtatccag ttaatggcag tcggtctgga tattttgaga aaatgacagg
    13381 cctcatcgag tgtgaggctg tatggctcca ttatttcacc tcttgctgtg acattgttga
    13441 aaaatggata ccagctcgtt gctgccagac gatccaaccg agagtcatat cccatgccat
    13501 gtattcgtta tcgccgtttt ttgctctccg acgatctact aagtcaccga aacgcttttc
    13561 catgaataat tcataggctt cgcgttcatc tggctctact tccagagata cgagtgcgat
    13621 ttcataagca cggcgctcaa tatcgtctcg aacctctagg ctgctgattc gttctttgat
    13681 ttctttaatc agttctttat tggtaaatgt ggtcattatg ctccagcctc cggtgctttt
    13741 ggcattactg cccagtgagt gatattgacg ttttcaaggt ccccgacctg aaatgtccac
    13801 tgccattctc cggtttcttt ttgtccccag gtgtaccaga gagaacgcca gccaatcagc
    13861 cagccttctc cattagcatc aaataacaga acactttcat ttgctggtgg cagttcagct
    13921 gacactggta ttattttgtt ttccagtgcc gcacatttag cttcaagcgc gtcgaattta
    13981 cgtaacaggt actcagcatt tgtttcgttc actttcagat ctcgcggtac acatttcccg
    14041 cgaagaaacc cttccatttc gaaaacattc atgcgcattt gcgtaactcc gataaatcgt
    14101 taaaacgttc cataaacatc ccgtaggcat gacccggtgc cagtggaatc acgttgaaca
    14161 tctctgttgc cgggatgcct tccagtacag gccagaaaga gccatcatca agcccgagat
    14221 cgcggcgttc ggttgccagc atgatgagat cggcatattt cacgggtgta ctcataactg
    14281 ggggtaaccc gtatttctca cggattacgg cgtctatttt ttcttccatc cgtttatagt
    14341 caggaagaag gcgtttcagt ggtgcgggaa tgtcctggca atacgcttct gttgcatcat
    14401 gcattaacgc ttcaaaagca aattcctgcg gcaccagctg gctgcaaaga accgcatgtt
    14461 gggcgacgct gtagaagtgc gaaagatgac cggcaaagcg acagatattt gaaagggaaa
    14521 c
//
LOCUS       DAZKHK010000123        14306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKHK010000123 DAZKHK010000000
VERSION     DAZKHK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.123"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            76..891
                     /gene="gspC"
                     /locus_tag="ACHOS5_003969"
     CDS             76..891
                     /gene="gspC"
                     /locus_tag="ACHOS5_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9778709.1"
                     /translation="MPTLPFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NEQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQEIPKLSIALNGIVLTSND
                     ETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            875..2827
                     /gene="gspD"
                     /locus_tag="ACHOS5_003970"
     CDS             875..2827
                     /gene="gspD"
                     /locus_tag="ACHOS5_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP9778710.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRVQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2837..4318
                     /gene="gspE"
                     /locus_tag="ACHOS5_003971"
     CDS             2837..4318
                     /gene="gspE"
                     /locus_tag="ACHOS5_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9778711.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVIRGDTSWDEVMRVASATLENEA"
     gene            4315..5511
                     /gene="gspF"
                     /locus_tag="ACHOS5_003972"
     CDS             4315..5511
                     /gene="gspF"
                     /locus_tag="ACHOS5_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9778712.1"
                     /translation="MNYRYRAMTLDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5521..5958
                     /gene="gspG"
                     /locus_tag="ACHOS5_003973"
     CDS             5521..5958
                     /gene="gspG"
                     /locus_tag="ACHOS5_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9778713.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5966..6475
                     /gene="gspH"
                     /locus_tag="ACHOS5_003974"
     CDS             5966..6475
                     /gene="gspH"
                     /locus_tag="ACHOS5_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9778714.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVSSGSWPRD
                     QTLARDTRP"
     gene            6472..6849
                     /gene="gspI"
                     /locus_tag="ACHOS5_003975"
     CDS             6472..6849
                     /gene="gspI"
                     /locus_tag="ACHOS5_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9778715.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6842..7429
                     /gene="gspJ"
                     /locus_tag="ACHOS5_003976"
     CDS             6842..7429
                     /gene="gspJ"
                     /locus_tag="ACHOS5_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9778716.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7422..8405
                     /gene="gspK"
                     /locus_tag="ACHOS5_003977"
     CDS             7422..8405
                     /gene="gspK"
                     /locus_tag="ACHOS5_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9778717.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLNTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8420..9583
                     /gene="gspL"
                     /locus_tag="ACHOS5_003978"
     CDS             8420..9583
                     /gene="gspL"
                     /locus_tag="ACHOS5_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9778718.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKFSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLSILSFVGSRGIAFWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9580..10041
                     /gene="gspM"
                     /locus_tag="ACHOS5_003979"
     CDS             9580..10041
                     /gene="gspM"
                     /locus_tag="ACHOS5_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP9778719.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            10041..10718
                     /gene="gspO"
                     /locus_tag="ACHOS5_003980"
     CDS             10041..10718
                     /gene="gspO"
                     /locus_tag="ACHOS5_003980"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFP9778720.1"
                     /translation="MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10747..11223)
                     /gene="bfr"
                     /locus_tag="ACHOS5_003981"
     CDS             complement(10747..11223)
                     /gene="bfr"
                     /locus_tag="ACHOS5_003981"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP9778721.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11295..11489)
                     /gene="bfd"
                     /locus_tag="ACHOS5_003982"
     CDS             complement(11295..11489)
                     /gene="bfd"
                     /locus_tag="ACHOS5_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP9778722.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11658..>14306)
                     /gene="chiA"
                     /locus_tag="ACHOS5_003983"
     CDS             complement(11658..>14306)
                     /gene="chiA"
                     /locus_tag="ACHOS5_003983"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFP9778723.1"
                     /translation="ALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRYVRA
                     ATAMEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWKGQE
                     GSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEITQYGNPGSC
                     SVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGSEIY
                     HVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTATAAEMSQYGNPTTCEIDNG
                     GVIIADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSAS
                     TVYVKGDRVVVDGQAYEALFWTQSDNPALIANQNATGSNSRPWKPLGKTQSYSNEELN
                     NAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPK
                     KAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQ
                     NYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRVLDF
                     DIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDA
                     KAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHAQLKGLFGNKSDA
                     EIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNLS
                     PEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3694 a         3672 c         3376 g         3564 t
ORIGIN      
        1 tgcaagcggc attaatacta tttatactaa cgtcaatata caacccaccc aatctatttt
       61 attagaacat acatcgtgcc cacactacct ttccctttcc atctggcgaa tcataataaa
      121 gatgctgcca tcaatatatt gattatattt ataagcatag ggttgattat ttttaatgtg
      181 aattatttcc atacaactat agttaaaaat gaacagatca ttaatcaacc aacaaatgca
      241 tttcagtctg aattcagcct tgccgcttta tggcgcaacg aaaatcatgc tggggtaaaa
      301 gatgcaaatc ccgtggcagt taatcaggaa atacctaagc tctctatagc gcttaatggc
      361 attgtactca catcgaatga tgagacttct tttgtcttaa ttaatgaggg gaacgaacaa
      421 aagcgatact ccctcaacga agcccttgag tcagcgccgg gaacgtttat tagaaaaata
      481 aataaaacca gcgttgtgtt tgaaactcac gggcattacg aaaaagtaac tctccatcca
      541 gggctacctg acatcatcaa gcagcctgat tcggaaaatc aaaatgtact cgccgattat
      601 attatcgcca cacctatacg tgatggagaa cagatatacg gtcttcgatt gaatcctcgt
      661 aagggtctca atgcatttac caccagcttg ctgcaacctg gggatatcgc cctcaggatc
      721 aataatcttt cgctgaccca ccctgatgag gtttcacagg cattaagttt actgctaact
      781 caacaaagtg ctcagtttac aattcgtcgc aacggcgtac cccgcttgat aaatgtttcc
      841 gtcgcggaac ttacaggaat gaatggacag cgcaatgaag ggactcaata aaatcacctg
      901 ctgcttgctg gcagcactac tcatgccttg tgcaggacac gctgagaacg aacaatacgg
      961 cgctaacttc aataacgccg atatccgcca gttcgtggaa atagtgggtc agcatcttgg
     1021 caaaacgatc ctgatcgacc cttcggtaca gggaaccatt tccgtacgca gtaatgatac
     1081 gtttagccaa caggagtact accagttctt tttaagtatt cttgatcttt acggttattc
     1141 cgtgatcaca ctggacaatg gttttctgaa agtggtgcgc tcagctaatg taaaaacatc
     1201 gccagggatg attgctgaca gttctcgtcc aggcgtaggt gatgagttgg tcacccgaat
     1261 cgtaccgctt gagaacgttc ctgctcgtga cctggctccc ctgctccgcc agatgatgga
     1321 tgcgggtagc gtcggtaatg ttgtgcatta tgaaccctcc aacgttctta ttctgaccgg
     1381 tcgtgcctcc accattaata aactgattga agtcataaag cgcgttgatg tcatcggcac
     1441 agagaagcag caaattattc atctggaata tgcatcagcg gaagatctcg ccgagattct
     1501 taatcaatta atcagcgaaa gccacggtaa aagccagatg cctgccctcc tttccgcgaa
     1561 gattgtggcg gataagcgaa ccaactctct tatcatcagc gggccggaaa aagcacgcca
     1621 gcgcataact tcattactga aaagccttga tgtagaagag agcgaggaag gaaatacccg
     1681 ggtctattac ctgaaatatg ctaaagccac gaatctggtg gaagtgctaa ccggtgtttc
     1741 cgaaaagctg aaagatgaaa aagggaattc gcgtaagcct tcttctactt ctgcgatgga
     1801 taatgtggcc attaccgccg atgaacagac caactcgttg gtcattaccg ctgaccagtc
     1861 cgtccaggag aaactcgcca cggtaattgc gcgtctggat attcgccgtg tacaggtgct
     1921 ggttgaggca atcatcgtcg aagttcagga tggaaatgga ctcaacctcg gcgtgcaatg
     1981 ggcgaataaa aacgttggcg cacagcaatt taccaatacg ggattaccgg tttttaacgc
     2041 tgcgcaaggt gtagctgatt ataaaaagaa tggcgggatc accagcgcga atcctgcctg
     2101 ggatatgttt agcgcctaca atggcatggc tgcaggcttc ttcaatggcg actggggcgt
     2161 attgttgact gcgctggcca gtaacaataa aaatgacatc ctcgccaccc cgagcatcgt
     2221 aacgctggat aataaactcg cgtccttcaa cgttggtcag gatgtgccgg tgctatccgg
     2281 ctctcagacc acttctgggg ataacgtctt taatactgtc gaacgcaaaa cggtgggcac
     2341 aaaactcaaa gtcactccgc aggtcaatga aggcgacgcg gtactgctcg aaatagagca
     2401 ggaggtttcc agcgttgact cttcgtctaa ttcgacgctc ggcccgacat ttaacacccg
     2461 tactattcaa aacgccgtgc tggttaaaac cggtgaaacg gtggtcctgg gcggattgct
     2521 ggatgatttt tctaaagagc aagtgtcaaa ggttcctctg cttggcgata ttcctttagt
     2581 gggacaactc ttccgctata cctcgaccga gcgcgctaaa cgcaacctga tggtatttat
     2641 ccgcccgacg attatccgtg acgatgatgt ttatcgctca ctgtcaaaag agaaatacac
     2701 ccgctaccgt caggagcaac agctgcgaat cgacgggaaa tcaaaagcgc tggttggctc
     2761 ggaagatttg ccggtgctga atgagaacac gttcaacagt cacgcccctg cgccatcgtc
     2821 acggtgaggc attcttatga gaattcactc accgtacccc gccagttggg cgctggcaca
     2881 acgaattggt tatctctatt cagagggcga gattatttat ctcgccgata cgccattcga
     2941 acggttactc gatattcaac gtcaagttgg ccagtgccag accatgacca gcttgtcaca
     3001 ggctgatttt gaagcccgac tggaagcggt gttccatcag aataccggtg agtcgcaaca
     3061 gattgcgcag gatatcgatc aatccgtcga tcttctctcg ctttcggaag agatgcccgc
     3121 aaatgaagat ctcctgaatg aagattcggc ggcaccggtt atccgcttga tcaatgctat
     3181 tttgagtgag gccatcaaag aaaccgcctc cgatatccac attgaaacct atgaaaaaac
     3241 aatgtcgatc cgttttcgca tcgacggcgt tttgcggaca attttacagc ccaacaaaaa
     3301 actggcggca ctgcttatct cccgaattaa ggtcatggct cgtcttgata tcgccgaaaa
     3361 acgtattcca caggatggaa gaatcagttt gcgtatcggg cgacgtaaca tagatgtccg
     3421 cgtatccaca ctgccatcca tctatggtga acgcgctgtg ctccgcctgc tggataaaaa
     3481 cagcctccag ctttcattga acaacctggg gatgacggca gcggataaac aggatttaga
     3541 aaatctcatt cagcttccgc acggtattat cctggtaaca ggaccgacag gctccggtaa
     3601 aagcaccacg ctctacgcca ttctttcggc gcttaatacc cctggccgta atattctgac
     3661 ggtagaagat cccgtggaat atgaactgga aggcatagga caaacgcagg tgaatacccg
     3721 agtggatatg tctttcgctc gcggcctgcg cgccatactt cgccaggacc cggatgtcgt
     3781 catggtgggg gagattcgtg atacagaaac cgcgcaaatt gcggttcagg cctcgctcac
     3841 aggccatctg gtactctcaa cactccacac taacagtgca tcaggcgcag tcacccggct
     3901 acgcgacatg ggcgttgaat cattcctgct ttcgtcttca ctggcaggga ttatcgcgca
     3961 acgtctggtt cgtcgtctgt gtccgcaatg ccgacaattc acgcccgtat cgccccaaca
     4021 agcgcagatg tttaaatatc atcagctcgc ggtgacaaca attggcactc ccgtaggttg
     4081 ccctcattgc catcaatccg gttatcaggg gcgcatggcg atccacgaaa tgatggtggt
     4141 gacgccggaa ttgcgggccg ctattcatga aaatgtggat gaacaagcac tggagcgact
     4201 ggtccggcaa caacacaatg ccttaatcaa aaatggcctg caaaaagtga tacgcggtga
     4261 cacctcctgg gatgaggtta tgcgcgtcgc cagtgccacg ctggagaacg aagcatgaat
     4321 tatcgctatc gcgccatgac cctggatggt caaaaattgc aggggatcat tgatgctaac
     4381 gatgaacgtc aggcacgact gcggctgcgt gaagaagggc ttttcctgtt ggatattcgc
     4441 ccccaaaaaa gttcgggagt aaaaacacgt cgcccgagga tcagccatag tgaactgacg
     4501 cttttcaccc ggcagttggc aaccttaagc gcagcggcat tacccctgga agagagcctt
     4561 gccgtaattg gtcaacaaag cagtaataat cgactggctg acgtgttaaa tcaggtacgc
     4621 agcgccatcc ttgaagggca tcccctttcc gatgcgttac agcattttcc cacgcttttc
     4681 gattcgctct accgtaccct ggtaaaagcg ggcgaaaaga gcggtctgct ggccccggtg
     4741 ttggaaaagc tggctgatta caatgaaaac cggcagaaaa tccgcagcaa gctcattcag
     4801 tcactgatct acccctgcat gctcactacg gtggcgattg tggtcgtgat tattctcctc
     4861 actgctgtcg tgcccaaaat taccgaacag tttgtgcata tgaagcagca actgccactg
     4921 agtacacgca ttcttttagg tctgagcgac acgttgcaac ataccggccc gacattacta
     4981 gcgacagtgt ttattgtcgc tgtaggtttc tggctctggt taaaacgcgg caataaccgc
     5041 caccgttttc atgccatgtt gctgcgcgtt gcactcatcg gcccgctgat ttgcgccatt
     5101 aacagcgcac gctatctccg cactttaagt attttgcaat ccagcggcgt ccctctgctg
     5161 gatgggatga atttgtccac cgaaagtctc agcaacctcg aaatccgcaa gcgtctggca
     5221 aatgcggcag aaaacgttcg gcagggtaac agcattcatc tttcgctgga acaaaccgca
     5281 atcttcccgc cgatgatgct ctacatggtg gcctctggcg aaaaaagcgg gcagctcggc
     5341 acattgatgg tcagggccgc agataaccag gagacactcc aacaaaatcg gatcgcctta
     5401 acgctctcca tcttcgagcc cgcactcatt attacgatgg cactgatcgt cctgtttatt
     5461 gtcgtctcgg tactccaacc tcttcttcaa cttaactcaa tgattaacta aggaaaaaca
     5521 atgcgcgcaa cggataagca acgcggtttt acattactgg aaattatggt ggtgatcgtc
     5581 attatcggcg tacttgccag cctggtggtt cccaacctga tgggcaataa agaaaaggcg
     5641 gataaacaaa aagccgtcag cgatattgtg gcgctggaaa acgccctgga tatgtacaag
     5701 ctcgacaatc atcgctatcc aaccacaaat caggggcttg aatccttagt cgaagcgccg
     5761 acactgccac cgctggctgc aaactataac aaagaaggtt atatcaagcg tctgcccgcc
     5821 gatccctggg gcaatgatta tgtccttgtt aatcctggtg aacacggcgc atacgatctg
     5881 ctttcagcag ggccggacgg tgaaatggga accgaggacg acatcaccaa ctggggtttg
     5941 agcaagaaga aaaaataagt cagcgatgaa tcagcaacgc gggtttaccc tgctggagat
     6001 gatgctggtg ctggcgctgg tcgctatcac ggcaagtgtg gtgctcttca catatggtcg
     6061 ggaggatgcc gccagcactc gtgcgcgcga aacagcggcg cggtttaccg ctgcacttga
     6121 attagccatc gaccgcgcca cgctcagtgg tcagcctgtc ggtatccatt tctctgattc
     6181 agcatggcgc attatggtac cgggcaaaac accatcagcc tggcgctggg ttccattaca
     6241 agaagatgcc gcagacgaga gcaaaaatga ctggggtgaa gaactctcaa ttcaacttca
     6301 gccatttaaa cctgatgaca gcaaccaacc gcaggtggtg attctggccg acggacaaat
     6361 cactcccttc tctttgttga tggctaatgc cgggaccggt gagccgctcc tgacgttagt
     6421 cagttcaggc tcatggcccc gggatcaaac gctcgccaga gatacccgcc catgaacaaa
     6481 caatcaggga tgacactgct tgaagtctta ctggcgatga gtattttcac cgctgttgcg
     6541 ctgactttaa tgagcagtat gcaagggcaa cgaactgcca tcgagcggat gcgtaatgag
     6601 acgctggcat tgtggatagc ggataaccag cttcagtcac aagattcatt cgacgaggaa
     6661 aacacgtcca gttctggcaa agagttaata aatggcgaag agtggaactg gcgcagcgat
     6721 atccactcaa gcaaagacgg cacgcttctg gaacacacca taacggtgac attacccagc
     6781 ggtcagacaa cctcgcttac gcgctatcaa agcatcaata ataaatccgg gcaggcacaa
     6841 gatgattaat cgccagcagg gtttcacatt gctggaagtc atggcggcgc tggcgatttt
     6901 ctcaatgctt agcgtgctgg cgtttatgat tttttctcaa gcttccgagt tgcatcaacg
     6961 ttcacagaaa gaaatccagc agttcaatca gctacagcgc accatcacca ttcttgataa
     7021 cgatctcctg caactggtcg cccggcggaa tcggagtacg gacaaaatca tggtgttggg
     7081 tgaagaagcg atttttacca cgcaaagtcg cgatccgctg gcccctctca gtgaagcaca
     7141 aaccttactc actgttcact ggtatctgcg aaatcatacg ctctaccgtg ctgttcgtac
     7201 ttctgtggat ggcaggaagg atcaacccgc ccaggcgatg ctggaacatg tcgagagctt
     7261 tcttctggaa agtaacagcg gagaaagcca ggagcttcct ctctcagtca cgttacatct
     7321 gaagactcag caatatgggg cgttacagcg ccgttttgca ttacctgagc agttagcgag
     7381 agaagagtcg ccagctcaga cgcaggccgg gaataataac catgaataat gaacaacgcg
     7441 gcgtagcact gctcattgta ctcatgttac tggcgctcat ggctgctctg gctgcagaca
     7501 tgacgatcag ttttcacggc cagcttcatc gaacccgcca ggtaaaccat catttgcaac
     7561 ggcaatatga tattgagctg gcggagaaac ttgcgcttgc cagcctcacc ctggacgtta
     7621 aagataacga cagacaaacc acgttgcagc agtactgggc acaaccgcaa cagttgcagc
     7681 tggaaaatgg caatacagtg aagtggcagt tacgcgatgc ccagcactgt tttaatctca
     7741 atgccttagc aaaaatctct gacgccccgc tggcttcccc tgatttcccc gcccaggtat
     7801 tcagtgccct gctgattaac gccggtatcg atcgcggaaa caccgatgag atcgtgcaat
     7861 caattgctga ctatattgat gccgatgatt cgccacgctt tcacggcgca gaagataact
     7921 tttaccaaag ccaaacgccg cctcgccata gcgccaatca gatgcttttt ctgaccggtg
     7981 aattgcgtca gataaaaggc ataaccgaaa acatctatca gcggcttatc ccgtatgtct
     8041 gcgttctccc gacctcggaa cttagcatca atcttaatat gctaacggag aacgatattc
     8101 cgctcttcag agcattattc ctcaataaca tcacggatgc tgacgctcgg gtcttattac
     8161 aaaaaaggcc cagggaaggc tggctaacca cagatgcatt tctttactgg gcgcagcagg
     8221 atttctccgg cgtgaagccc ctggttgctc aggtgaaagg gcatctcttt ccctatagcc
     8281 gctattttac gctgaatacg gaaagtatca gcgacgaaca atcccaggga tggcaaagcc
     8341 atattttttt caaccgtaaa cagcagagcg cccaaatcta tcggcgtacg ctgcaactct
     8401 actaatcata aggatcgtga tgcctgaatc gttgatggta atacgttctt tctcgacgct
     8461 gcgtaagcat tgggaatgga tgacgttctc cgcagatagc gtatcttcgg tacatacgct
     8521 cacagacgat cttccgctgg agtcgctggc cgatcagccc ggtgccggaa atgttcacct
     8581 tttgatccca cctgagggtc ttttatatcg ttccctcacg ctgcccaacg ccaaatacaa
     8641 actcaccgcg caaacattac agtggttggc ggaggagaca ttacccgacg ccagccaaaa
     8701 ttggcactgg acggttgtcg ataaacaaaa cgaaagtgtc gaggtgatag gcattcagtc
     8761 ggagaaattc agtcgttatc ttgagcgttt acatactgcc ggactcaacg tgacgcgggt
     8821 tttgccagat ggctgctatc ttccctggga agtagatagc tggactctgg ttaaccagca
     8881 aacaagctgg cttattcgca gtgccgcaca tgccttcaac gaactggatg aacactggtt
     8941 acagcatctt gctaaccagt ttccgccaga gaatatgcgt tgttatggtg tagcgcctca
     9001 cggcgttgca gcggccaatc cacttatcca gcatcctgaa ataccatcac tgagtttgta
     9061 ttctgccgat attgcattcc agcgctatga catgctgcat ggtgtttttc gtaaacagaa
     9121 aactgtaagc aaatcgggaa aatggctcgc ccgcctggca gtaagctgtc tcgttctctc
     9181 cattctttct tttgttggca gccggggcat cgcgttctgg caaacgctta agattgaaga
     9241 tcaacttcag caacaacagc aagaaacctg gcaacgttat ttcccgcaga tcaaacacac
     9301 ccacaacttt cgtttttact ttaagcagca actcgctcag caatatcctg aagctgtacc
     9361 gttgctctat catttacaaa cacttctgct tgaacaccct gaactgcagt taatggaggc
     9421 gaactatagc cagaggcaaa agtcgctgac cttaaaaatg agtgctaaga gtgaagccaa
     9481 tatcgatcgt ttctgtgagt taacacagtc ctggcttccg atggaaaaaa cagaaaaaga
     9541 tccggtcagt ggtgtatgga cagtaaggaa ctcagggaaa tgattaaatc atggtgggca
     9601 gaaaaatcca catcggaaaa acagatcgtt gcggcactgg cggttctctc gctcggcgtt
     9661 ttttgttggc ttgcggtgat aaaaccgata gatacctaca ttgaagagca tcagtcccac
     9721 gcgcaaaaaa ttaagaagga catcaaatgg atgcaggatc aagcgagcac ccatggttta
     9781 ctcggtcatc cagcgcttac gcagcccatc aaaaatatcc ttctcgaaga agcaaaacgc
     9841 gagaatttga ctatcacgct ggaaaacggt ccggacaata ctctgacaat taaccctgtc
     9901 acagctcccc tcgaaaacgt ctcccgctgg ctcactacgg cacagataac atacggtatt
     9961 gttattgaag accttcagtt tactcttgcc ggaaacgaag agatcaccct caagcatttg
    10021 tccttcaggg agcaacaata atgacaatgc tactgccgct cttcattctg gttggtttta
    10081 ttgcaggtta ttttgttaat gtcatggcct atcacctctc gcctttggag gataaaacgg
    10141 cgttaacgtt tcgccaggta ttggttcatt tctggcaaaa aaaatatgcc tggcatgata
    10201 cagtgcccct gatactttgc gttgctgctg ctatcgcctg cgccctggca cctttcacgc
    10261 ccattgtgac tggcgcactc tttctctatt tctgtttcgc actcacgctc agtgttattg
    10321 attttcgcac tcagctcctg cccgacaaac tcaccttacc gctgctctgg cttggcttgg
    10381 tatttaatgc gcagtctgga ttaattgatt tacatgatgc ggtttatggc gcggtcgctg
    10441 gctatggggt gctgtggtgt gtttactggg gcgtctggtt agtttgtcac aaagagggat
    10501 tgggctacgg tgatttcaag ctactggccg ctgcaggcgc gtggtgtggc tggcaaacgt
    10561 tgccaatgat actgctgatt gcctcgctgg gtggcattgg ttacgccatc gtttcacaac
    10621 ttctgcaacg ccggactata accactatcg cattcggccc gtggcttgcg ctcggcagca
    10681 tgataaacct gggatatctg gcctggatct cttattaacc gggagggttc gccctcccga
    10741 cacggctcaa ccttcttcgc gaatctgtgc ttgcagataa ttttgcagtc ccatcttctg
    10801 aatcagatca agttccgttt ccagccagtc gatatggcct tcttcatcgc gcaaaatttc
    10861 tatcatcata tcgcggctga cgtaatcatg aacgctatcg gcataaccaa ttgcctcacg
    10921 caaattcttc gcgccatcca gctcaagtgc cagatcagaa cgcagcattt cctcaacatc
    10981 ttcaccaatg ttcagtttgc ccaggtcctg taagtttgga agaccttcca gaaaaagaat
    11041 gcgctcaata taacgatcgg cgtgtttcat ctcatcaatg gattcatgat actccacatc
    11101 attgagacgt ttgagacccc agtttttaaa catccgggca tgaagaaagt actgattgat
    11161 tgcgacaagc tcatttccca acagtttgtt gagataattt ataactttag tatcaccttt
    11221 cattttttga ctccttcgct tccactcttt gaagcgtaga acggcttacg agtaagtcaa
    11281 aaaacacact ccgtttatgc ggactcctta aactccggca gttgcattaa ttcatcctcc
    11341 atcacttcac gcgcggcccg gacacattta ccgcactgat tgccgaccgg aatgaatttt
    11401 tttaattgct ggaacgagtg gggggaaaac tggcgcacag cctgacgaat ttttttatcg
    11461 ctgataccat tacaaagaca aacgtacata gagactcccg tatactttct tcaaaagtaa
    11521 atgaaaatag ttcttatttc aattacagaa agactctttt ttaagcaaag aatagaaata
    11581 tgaataaaaa taatgggcta ccaaaagtag cccattgaca aaaaatgcgg cgataccgga
    11641 aggtatcgcc aacacattta ttgcttagta aacggcgcga agatttcgct aaatgcgtac
    11701 ttcggagcct gttctttagt cagaccgtgg aattccggag acaggttagt gccgcccggc
    11761 aggtcgcgcg cgattgacca gataccaacc ataccgagat tacgcttctg cgcatcctgc
    11821 atgaccagac gagcatcaga gaggtagaac acctcaccct gaacgtcgtt cacgccaacc
    11881 atcggcgtgg tacccatcat ggcgtcaatt tctgcatcac tcttattgcc aaagaggcct
    11941 ttcagctgcg catgcaggtt ggcaatcgca gacgttgcac acttaccgtg gatgttctgg
    12001 ccttcggtgt ttgcagactg acaaatagcg ttaccgtagt ccattgtcat cacgttcaca
    12061 cccgccagct caacaccttt cgctttggca tcgctcagga cattcatccc ttccggcgtc
    12121 aggcctgtcg gcagaattgg caaggtatac cagatagcaa tatctttgcc ttctgacttc
    12181 catttatcct gcactttctt cacagcaagg ttacgacgtt caatagatgc ctgatccgca
    12241 acccaggtgc cttcgatatc gaagtccagg actctgaggt tcaggttatc aacgatgtca
    12301 taataatgct gcattagatc gtcaacgttc ttacaggaag ctgccagcgg ggcattatta
    12361 gcaccaccga tagacagcat cacatcaccg ccagcctcac gcagcgcttt gattttgctg
    12421 tactgagcgt aattctgcat accgtaagcg gtaccccatg tcggtagaca ggtgttcgca
    12481 tctttactca ccacaaacgc cagcgtgaag tggttgacgt tatgattctt agccagcgca
    12541 gccaaatccg ggatcgtatt cagcgtaaag tcgacatacg gtgcataaac gtgtttcggc
    12601 cacgctttct tcggcgtacc tacgcgctct ttggttccgg tccagtcaac aaaaacacgc
    12661 cacgggttgc tgtcagaagg agaaactttc tgcactttac tctgagaaat gtagttctca
    12721 ccgttaaagc gaatcagcgt atcactggca taaagcgttt ctggattaaa ctgcggcgca
    12781 ttattcagct cttcgttgct atagctctga gtcttaccta acggcttcca cgggcgacta
    12841 ttgctaccgg tggcgttttg gttcgcaatc agagcagggt tgtcactttg cgtccagaac
    12901 agcgcttcat aagcctgccc atcaacaaca acgcgatcac ctttcacgta cacggtggaa
    12961 gcggaccatg ctttcgccgg ttcgtatttc ttccacgggt tatcgccgcc ggggacaaag
    13021 ccccatgcgt cttgatcgac agaggtttta taatgtgcat cgttataatc tacgatgctg
    13081 ttcgcgctgt acgctttgct ggcctggaaa ccatccgcaa taatgacgcc gccattatca
    13141 atttcacagg tagtcgggtt accgtactga ctcatttccg cagcggtagc tgtacgcacg
    13201 aggcgccatg ggttattgga gttttctaca ctggtgccac gcgggcaatc ttcagaacca
    13261 acccaccagg cattctgata aacattacca ttgaagataa catggtaaat ttcgctgcct
    13321 tcctggccac tccacgcctg cagtgagtaa tcgttactgc tatccgctgg tgttgatggc
    13381 tcgttgttat ccggagttgg agtaactgga gtttccggtg tttccggagt tggatcaacc
    13441 ggagtcacag caccgccatt attatccggc ttaacggaac aggagcctgg gttaccatac
    13501 tgagtgattt ctgttgccgt tgccgcgcga acataacgcc atgggttgct ggcatcgttt
    13561 tctttcgcat cgcctgggca atttgccgcg cccacccacc aggcattctt ataaatgccg
    13621 ccgttgtaga taacgtacca tgtagaagaa ccttcctgac ctttccaggc tacgacagca
    13681 tagttgtcgt ttgacttatc agctggggct ggctgaacat caggagccgg tttaacgtcc
    13741 ggtgccggtt tcacatccgg tgcaggctgt ggtgctgaag tacaagactg tggattactg
    13801 gtttctgaga tttccattgc cgtagcggca cgaacataac gccatgggtt gctggcatca
    13861 ttgcctttcg catcacccgg gcagttagaa gaggctaccc accaggcatt tttatatacc
    13921 gcaccattaa agacgacata ccaggtttga ccgccctgct gtttattcca ggcaactaca
    13981 gaagaattag acggaacagt gccctgtgct ggtgtagccg aaccgccatt agcaggcgta
    14041 ttagatgctg gcgtatttga atttgaagaa gatgagctgc cgctcttttc gcaggaaagt
    14101 gtattgccaa actgactaat ttcagcagct gttgcggtac gctttaaacg ccacgggtta
    14161 gttgcatcat ttgctttcgc ttttcccggg caatttgtag aagaaaccca ccaggcattt
    14221 tcataaattt tgccatcgaa aataacctga tatttattac caccttgttg gttattccat
    14281 gcttccattg ccaatgctgg cagagc
//
LOCUS       DAZKHK010000124        13929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKHK010000124 DAZKHK010000000
VERSION     DAZKHK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.124"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHOS5_003984"
     CDS             complement(<1..209)
                     /locus_tag="ACHOS5_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HFP9778724.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENE"
     gene            complement(255..1085)
                     /locus_tag="ACHOS5_003985"
     CDS             complement(255..1085)
                     /locus_tag="ACHOS5_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9778725.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDSSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            complement(1089..2561)
                     /locus_tag="ACHOS5_003986"
     CDS             complement(1089..2561)
                     /locus_tag="ACHOS5_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9778726.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            complement(2610..3485)
                     /locus_tag="ACHOS5_003987"
     CDS             complement(2610..3485)
                     /locus_tag="ACHOS5_003987"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9778727.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEVCNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            complement(3530..4435)
                     /locus_tag="ACHOS5_003988"
     CDS             complement(3530..4435)
                     /locus_tag="ACHOS5_003988"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9778728.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGILNTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKK"
     gene            4552..5104
                     /locus_tag="ACHOS5_003989"
                     /pseudo
     CDS             4552..5104
                     /locus_tag="ACHOS5_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5338..6024)
                     /locus_tag="ACHOS5_003990"
     CDS             complement(5338..6024)
                     /locus_tag="ACHOS5_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9778729.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(6136..7083)
                     /locus_tag="ACHOS5_003991"
     CDS             complement(6136..7083)
                     /locus_tag="ACHOS5_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP9778730.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYLALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            complement(7197..8174)
                     /locus_tag="ACHOS5_003992"
     CDS             complement(7197..8174)
                     /locus_tag="ACHOS5_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFP9778731.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKLYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            complement(8267..8650)
                     /locus_tag="ACHOS5_003993"
     CDS             complement(8267..8650)
                     /locus_tag="ACHOS5_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778732.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            complement(8766..9395)
                     /locus_tag="ACHOS5_003994"
     CDS             complement(8766..9395)
                     /locus_tag="ACHOS5_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778733.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            complement(9477..9854)
                     /locus_tag="ACHOS5_003995"
     CDS             complement(9477..9854)
                     /locus_tag="ACHOS5_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778734.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            complement(10050..10529)
                     /locus_tag="ACHOS5_003996"
     CDS             complement(10050..10529)
                     /locus_tag="ACHOS5_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778735.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(10612..11004)
                     /locus_tag="ACHOS5_003997"
     CDS             complement(10612..11004)
                     /locus_tag="ACHOS5_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778736.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
     gene            complement(11406..11729)
                     /locus_tag="ACHOS5_003998"
     CDS             complement(11406..11729)
                     /locus_tag="ACHOS5_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778737.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(11762..12664)
                     /locus_tag="ACHOS5_003999"
     CDS             complement(11762..12664)
                     /locus_tag="ACHOS5_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP9778738.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
BASE COUNT         3979 a         3711 c         2774 g         3465 t
ORIGIN      
        1 aattcatttt cattgttgtg aattgtattc caggtgtttg actcaaaact tgcccaccaa
       61 ccattattcc acccctgact aacctgaatc aacgtttcat aagcgtgact ggcgcttctg
      121 tatcccccac gtagattaag agttaatgcc tgagagcaca aaggtaaacc aagaagaatt
      181 acagttaata gcggataata ttttttcata aatgcctcat cattaaatat atttatcact
      241 tgtaatctga tttctcaatg aaacttatgt tccgcaagcc attccagaag caggctttgc
      301 catttttcta tcggatatcc attttttccg gatacaccaa atccatgacc tcccttatca
      361 aaaaaatgag cttctgcatg atgtccctta tcccggatag agttaaaata tctcagagag
      421 tgaataactg gaacatcctt atcatctgtt gcatgcacaa taaagactgg tggcatatac
      481 tctgtaacac tattttcaag actggctgaa tattcacttt cacctgcatc cagaagacgt
      541 ttccggcatc cctgatgagc aatactcctg tcaagagata taactggata tatcagagcc
      601 atccagtcag gacgcacaga agtctcttcc tcataatgga gatgagtaat atatccagca
      661 agatgaccac ctgccgaaaa ccccatacat ccaacactct taaatgttct ctccgacatt
      721 ccggcccagt aagaaataaa gttcagggca ttcttcgcat ccgcaaagga ataataacca
      781 ttaacggagg gcattctgta agaaagaata aatgccgtat atccctgacc tgctatccat
      841 ctgcctacat caagtccctc tctttcataa acaatcctcc tgtatccacc tccaggtata
      901 atcagcacac tatgttgatt agatacaaca ggctcaacaa ctgtaataag gggcacaata
      961 cctgtatata ttatgccatc gtcagtattc tcaaatagcg aactgtcagt tatgtaacgc
     1021 tcataattat caagagaata tatattctcc ctgtaatgaa tcattttttt attccctgat
     1081 agcatccttt attctcctgc aacattcttt tttcttgctc cataaaaagg ttttccatcg
     1141 acagtatcat attcaagcgc agcttctgga tggatccatc tctgtacacg tgatggcata
     1201 tcaaaaccaa taaggacaag aacaacaaat gttaagataa aagcaagaga acacattgct
     1261 gtaccaagac ttgtatgaga cgcaacaact gcaccaagaa taggcgcaca tgctccacca
     1321 agtgaaccta cattataaat aaaccccagt cctgccgcac gctgctccac attaaaataa
     1381 ccactgatta ttttcggcac cagagcacca atcccctgac caaaaacctg ctgagtaaag
     1441 atcagaaggc caagtaacca tacatagtcc cggtccacaa gaaaaacggg gataataaca
     1501 agctgaccga taagaagtga tataacatat gcttttcggg ttccaagcca gtcccccata
     1561 aaaccaccta tacaacttcc agctgcagag ccaaaacctg ccagcataaa tatgagagca
     1621 acagtttctg gtgaatactg catatcaacc ttaagccatg taggaagaaa agcctgcagt
     1681 ggccatccgt agaggaaaca gaacataacc acaagcatca acataatgcc tgttggccat
     1741 cttttcccca taccctgata tataaaactg ataaaaacag ccgcacagca caatgccata
     1801 actgtgatta aagcagcgtt tgcagtgacg cctgaaaaac aaacatatag acatgcaaat
     1861 gcaatacaag acagaagaat atttatgtat ttattctttt ctctgtacag aatatccacc
     1921 attgttctga cgggtttatt ctcttgttgc cttttctgcc agtcatcaga ctccggaaga
     1981 ttcttgcgaa gataaaaagc aaataatatt ggaagcatgc cgacaaagaa aagagaacgc
     2041 cagccccata gtggtactac aatgctataa acctgtgcag ctaaacctcc cccaatagaa
     2101 aatccagaaa ttaaaaaacc actcgccttg tttctgagat gaacaggcca gctttcgata
     2161 acatatgtta cacttgagcc atattcacct gccataccaa gcccaataac aatgcgagca
     2221 ataaacatca ctgcatatcc aggagccaga ccacaggcaa gggttccgag agagaatagc
     2281 actatactta aaaccattgc catcctgcgc cccatcttgt ccgataaagc gccaatagca
     2341 agccccccaa accatcggga aataaaagca gcggaaataa ggcttgccgc ttctattgtc
     2401 gtcaagccga attcatgctg aacctctgta agaaccagag atatcagaac gaaatcaaaa
     2461 ccatcaagta aataacctgt ccatgccgca ccgaatatct tccactgtct gggtgaaaga
     2521 tgcttatacc aaacctgctt tttctgaata caggagtcca tattaaacct cacaataata
     2581 gcggatttat aatacactgg agtattactt taagtaatac aatcccggtc ccaaagagtt
     2641 gcaccaataa gaccagagtc acttctgaaa tatgccggaa acagcattac attacaaact
     2701 tcagaatata tggaaagatg tttcgacact ttctgaatat acccatcagc aagtccaaca
     2761 cttcctccga caacaacaac ctgacaatca agcagtaatt ttaaacttac tattaattca
     2821 gcaatagtac tggcagaacg ctctgtcaaa aattctgctt ccttatatcc ctgtctcgct
     2881 aattcaaaaa tatatttggt gctatatcct gcaagttcat tttttgctgc ccccatgatg
     2941 gcccgaccag atgaaaccgc ttcaacgcat ccccgacgac cacatccaca ttctgtatcc
     3001 gttactccgg acaatatatg cccaacatgt ccggctaatc cagaaacacc tgttaataat
     3061 ttcctgttaa ctacaattcc ccctccaacc cccgtagaaa cggttataaa aaccatatca
     3121 caaatttctt ttggcaaaac tgtatattct gcccatgcgg ctgcctgggc atcatttatc
     3181 acacttccat ttaaaccagt tatatcttcc attatatttt tcagaggata ctctttcagc
     3241 cctccaagat tgtctggatt cagagctgtc aatactccat tattaataat ccctgttgat
     3301 gctactgcaa atcgctccgc tgttcccttc atttcagaaa ccagtagacg taaagcccct
     3361 gtcatttcgt caggacatct ttcatgaggt gttgatatct gttttttcag caaaaatgaa
     3421 ccgtcatcag atatcaacgc cgctgaaatt ttcgttccgc ctatatcaac agccagtgtt
     3481 atcattcatt ttcacctata taacaatctt gcaattattc agattatttt cattttttat
     3541 ttctgtcatg ttcttcgagc agtcgctctg caagttttga taatacatcc agattctttt
     3601 cctttaccgg agaaaaagga cttctgcata caggagtatt tagaatcccc atatagtaaa
     3661 gaagagtttt tatacctgcc aggacaccac tctgaataag agtgtcaata acctggttac
     3721 aggccacctg catttccttc gccttaatca catcattgtt tttgactgct tcaaatattt
     3781 caagataacg ccatcccatt atattatatg tactaccgat tcccccatct gcacctgcaa
     3841 ttaaccctga tgcaaatatt tcatcgtaac cattatataa cacaagctct ggatgattcc
     3901 ttttgatttg ttccatctgg aataaatcac cggatgtctg ttttaatgca caaaccccgg
     3961 gtattgtgac aagctcattt atctgatcta aagaaaaacg cactccgcta agagccggta
     4021 tattataaac taccatcgga agtccatctg cagaatcaat aatcttcctg taatggatac
     4081 aatgctcttc aaatgaaaaa ggataataaa atggagtaac agcagatata gcatgatatc
     4141 cgtatttttt tgcagctttt gcaagtacct cgctttctgc cgtactgatt ccgcccacat
     4201 gcgctataag tgttagtctt ccatctgatt catcggctac tgtttcaaga attttttctc
     4261 gctctgcaac attctgaaga aatgcttctc cagtagagcc tccaacatac aatcccttaa
     4321 tattttgttc gatattaaaa cggatcagtt ttcgaagact ctccatatcg atttgctggg
     4381 attggtcata aggagtgggt aatgctgata tgaccccttt aaattcacat tgcattttta
     4441 atcaccttca cttaactgca taatgggata cctagtacac caccaagtgc aaaaattatc
     4501 caacagaaaa gaagcacaat gtgaagatta tcaaaaaaat ccatcagaaa gctgtcatgc
     4561 cgctcagtta aggatcggtt gatcgatcca gtgtccagtt actattctct gcgtaaccct
     4621 ctgcgactat cttgccctgg aactgattac ccacagccct gaagagtccg acactgtcac
     4681 gctacacaaa cggcgtcttc ctctggaaat gatggtctgg tgaattatca gtatggccca
     4741 tgagcgtaag gaaccttcat cagattgtga accggctgga catcatgctg ccaggcagta
     4801 gcgccgtgct ccatgcccgc cagcggccag gaagcggagc catacgctgc gtgttcactc
     4861 aaacagcgca gctctggcat aacaccacgc catatccgct ctggtgcgat ctgaatctgc
     4921 tgaccattga tggcgtgttc tgacgaacac aggacacgca agaacacgat aaagcttcac
     4981 tcgccaaata catagtggaa accagacact ttatcctcag gtaaaaatgg tctgtctgat
     5041 agaactgacc agccatctac tgacggcagc ggccttcgat acaatgaatc tggcactaaa
     5101 taagactggc aatatagccg ataatgcgtt gaatcagtgg ctatatcgta tacataaaac
     5161 agacatcatt attctgtctt gttgtccaac acataaaaac taataaaaac ataccattcc
     5221 ctgtcgtcag agaatttatt gataaacagg ctttattgaa caaatcaaac attcagggaa
     5281 ctggcagttg aacaaaacgc cagtccgaag actggcgcag tgataaaaca ttctgcatca
     5341 tgccacattc tgctcctgaa gatgacgcag acgatggtcc cagtttccca ggtaatgccc
     5401 gggagtcagc agtacgcggc gtcgctcatt acagagcgag ttaccaatca ccacttctcc
     5461 ggccacacca cacaacgaca gttgtatata cgccatgccg gctgccagcg ggtcaatatc
     5521 cgttgccgac acccacatcc tgtggtatgc cggatacccc gaacggttca ggatgtcagc
     5581 cattgccaga atcatactgc cggcaccaca ggcaggttca ctgagcgtga taaagggttt
     5641 gttctcaaac aacgccttca catctcccat ctgcatctgt gccatcatgc tggcaatact
     5701 ccagggcgtg aaaaactgac tccggtattt atcccccagt tccagttgca tgaacacact
     5761 ccccagaaaa tcacagaatc cctgctccag tccctcaatg acatgagcaa gcaaatgcgc
     5821 aatgtggata acatcctgct tttcataacc actgacgatt cgcagatact tctgttcccg
     5881 ggcctcactg aactgaagcc ggttttccag ggcaatcaca ctacagctga caaagtcttc
     5941 aaacacctta tgacgtttgt gataacaggc tgtccggtta aacaacgaaa tgaactcctt
     6001 ctgatggtca gtcatggtgg ccatgttttc tccgtaaaaa cagggaacac atggcccgga
     6061 caggggaaca ggtgttcccc ttcgggttga aatgttgata aaacgggcat cacggacatg
     6121 atacccggag gttaattact gggctgagac cagaggaacc ggtaaaggtg cggctttcgt
     6181 ctgttcttca gcgcactcct ccaccagata cagtcggggc tgtggaagtg tttccctgcc
     6241 cggccattcg gcctgcagat cactgtccgt atgctctccg ccatcgtaac agttgtaacc
     6301 ctgtataccg tgtaccggct cgctgtacca gtgtcggacc tcgcagtcaa acaccttcga
     6361 gatctcacca accagttcgc ctgaaggcgg ataccagggc gtgtcgatca gcagcaccag
     6421 actgctcatc tcatcacgcg catggcgaac attatttcct gacggccact ccacaccgta
     6481 tatccggctg taaaggctgg tcgttgtgga aataccctgc agcagtccac ctgaaccatt
     6541 cagctctgta gccagacggg agggtatgac catcagcatg tcacagggct gcgcctgttc
     6601 cgggtaatcc gacaaccagc tccagcaggc ttctgcatca atgtgttcac tcagcccggc
     6661 cagaccaaac cagtcagaat actgacggct catcagcgtg gtgatgatct ccctggcatt
     6721 atccggcaga ttaccccagc gaacagcgcc gatacatgac tggtgataaa gacgctcaat
     6781 ggtctttatc gtttcaccat ccagaacggc attttttacc agcaacccca gccactgctc
     6841 aaacgcctgg ttagccggag atgacaaccc cgttccgtga cgaaccagcg ccagatatgg
     6901 cggatactgc gtcgtccgtg tgggttttac cagccctgca caaccggcca gaaacagctg
     6961 tatactctgc tgtatggcat gacggtagcg cggggtctca cgcccttcaa tccactgtgt
     7021 aagtacatca agacagactg atttaccggt aatttcaaaa cggtttttgc accattcaga
     7081 cattgtttac gctcctcttt tttatacaga tgtaaaaaga gggcattgtc ccgtgaggta
     7141 caatgccctc acgggataaa tggcggtcag gttataaccg ccaccggtag taaaaattat
     7201 gctgcgcact cctgcgaagt tcgccgcgtc agtaaatact ctgatgcttc acgtgcctgc
     7261 cggcaggcgc gaaacagcgc ttttttgtca gacttcagaa ccttaagcca tccgtcaata
     7321 taactttcgt gctgtacgtc accggcaatc ccgagatgtg cacacagaaa agcactgccc
     7381 atctcggcaa tcagctcttc aaacgcatat tccgggctgt tgcgaccggt aaaggtactt
     7441 atccccaccc gccgcaggcg cttttcatga ccggtactgt gtaccagctc atgcagcagg
     7501 gtggtccagt aatcagccac ctggtcaaac tgcgacgaca gtggcatcac gatacggtca
     7561 agatcaggcc ggtagaatgc cctgtcctgt ggaagtgacg agacgctgac gccgctgcca
     7621 ttcagtacct gcagcacccg ctcactgaag gtatctgccg gcagtgcatc cgcgctgcct
     7681 tccgccttca ccatcagatg ttcaggtaaa ccgtcacact gccccacatt aaacagagga
     7741 ttcgatttca gtaccggtac gatttccatg agaggctccc cctcactgtc cgtcagccgg
     7801 ttaccgtgtt catccacagc ctgttttttc cagtctttgt aaatcaccgc cagcgtggcc
     7861 acctcccctt tacggacgtg gccacccagt tgttgcgcct gacggtacgt cagccagtca
     7921 ttgctgacat acccggcttc atccgcggcc agccacagca acaggacgtt cacccctgta
     7981 taaagcttcc cggtcagggc atttgccggc atcatctccg gaagccccac gccattacgc
     8041 gcagagcgcc agggacgttt ccagggtggc acaccatttt caatggccgt aacaaccctg
     8101 tcggtcacaa gctgatacag gtcacgttcc ggagttttag tcgcggtacg tgaagtatgt
     8161 tttttcgttt tcataagagc atgccccctg aaaaagatgc acgctccctg tacggtgaga
     8221 gtgcaccgta cagttaaatt aataacgaaa atcagactgg ccagaattac cacaacgcca
     8281 gcatcaccag tgaacgacgc tcaaaatcaa acgtcttcag cgtattgctg acccacgctt
     8341 ttacgtcatc cacgtcatac cagtaatcac cggcataatc ttccccccgt aaacacgagc
     8401 ctgtgacata aggaaacagc ctgtggcagt tccccggtgt cagcatactg aggtcgcgca
     8461 ataatgcagc cagtgaactg cgggttacag ggaaagcatg ctgctcatct gaagcaccgg
     8521 tatgttcacg aaaccatgtc agaagcggtt tcaccttacg gaaagccccc acctcctgat
     8581 gttcctgtgt tcccacaacg gcaccactgt gatggttatc acgagccaga aagtaaatat
     8641 cgagtcccat aacaattctc ctgaataaag ccgggactct ccccacaggg atatgtccct
     8701 gccgggtgat ataatgacgg aatacgtgat gacgttattc cggataaaga aactacggcg
     8761 ttctgttacc gcccaccggc aagagcccgc agccagtaaa gctgatattc ggcctcttcc
     8821 agtgctgccc agtcatccgg ggataactgt cgcccctgac cggacaaaaa ctgcagcagt
     8881 aagctgtcaa acgcatctcc ccttaccggg gtggttaata cagactccgg agcagaaaaa
     8941 ttctcatgcc ccaggtaccg ttttatccat ttcagcggca gcgaccagct ggcaccaccg
     9001 acagtggaat gaatacgaaa acagtccgga cacagattgt gcaacagcac aaagactgta
     9061 cgggcaaaga aatctcccgg ctgacgtccg aaattcagct cattaagagc cagaatattc
     9121 cggctgtgct cccgctcaat agtgacattc accgaccaca gcccgtcggc agaccggcag
     9181 taaaacgcca gcgtgtccgt taccggaaac aactctgacg cccgggtcag ggagccgaaa
     9241 tacgcattct gcggccgtaa cgcctccggc aactgccaga aggtttcagt gacagccaga
     9301 acaaaagcat gccactgctt ttccggtatt gtggaaacct gctcaagggt atataccacc
     9361 ggctcacggg ccggacgtga agataactca gacatggaac attcctcatg gttaaaacga
     9421 aacaacgggg aatgtctccc ggacggagaa ctcccccgtc agggtcaggt catgaattac
     9481 atcatgtcgt gccgggcaaa atattcatcc gctccggcca ccatctttcc ggtactgata
     9541 ctgtcagccc agatttcatg attaatttca cgccccagag ccatcagact gtcgagtgcc
     9601 tgacgggcct gaattcccgg tgcggaaatc tcctcctgct ccaggtcata caacagccag
     9661 cccagcgcct cgccggtatc ccacagatac acgtatacca gtgaaggctc cgtctcatca
     9721 tccggctccg gggtaatacc cagccgttgc caggcacgta accgcatcag ctcgccttcc
     9781 atctgactga tatgagccag cagagcagac tgcgtctgaa gcgctgaata atgagcaatt
     9841 aaaggtgaat acatgatgac ctccgtctct taaaggaagt cctgtccctg acggaacaaa
     9901 actcccgtca gggaaaacac cggagcaaaa acacggtgca gtaagtaaaa gaaaaaccgt
     9961 tacgggagca aaacagccat cattgcaaaa tggcagttgc atcgctcacg atttgacatc
    10021 cttcctgtga ctgactggca tcctgcccgt cagtctgcgt cggaaaattc aatacaggcc
    10081 tgcttcagga gcccggctat acgttgattc agacattccg tccagccatc gttccatcca
    10141 cgctcccatg gattgcagac atcctcccat ggattgcaga catcgttaca gccattcttc
    10201 catgtcttcc atgcctccat ctccatgaca gcccccagcc tgtggccttt cacacgtata
    10261 tgccacagaa acggccggaa aagtcctgat ttcagttggt caccaccgct tttgtagccc
    10321 agatgacgtg caatactttc agtatattca aatgcatcat tctgcattcc agccgcctta
    10381 gccagcgagc tgtacgcatc acgaagtatc gtcatacctt ccagttgata ctcacaccac
    10441 gcacggacag ccgacactgg tgcgattacc cgaagaatgg tgcaggcacg ccatacaaca
    10501 acgtaaagga gtattaacag actatgcatg tgttcacctc agatacaaaa aataacggga
    10561 acacacctgc cctgcgggaa gcgtgttccc gacagggtaa taaatgataa atcagcaggg
    10621 ctgtgcgacg attccatcac cctgcagacc aaaccggtaa cgcagggtgg tcagttgctc
    10681 cgccatacgg caggcagcat gccatgtcct gcgacgggat atccgtccgg taaaccggtt
    10741 cagataaatg acctcacgcg gtaacggctg ataccatcct gtgtactcgt tgcgatacat
    10801 tccctgccgg taatacaggc agggtgtcca gacaccgcca cgacggaaca tacagacccg
    10861 cacgctcaga ttgccggccg gattagtgta ccagcgactg taatatgaca ggttattgcg
    10921 ggaaaaaaac acccgcaact gagccgtcat tcgccggaaa aaagaagaga tgcccggttc
    10981 agggtacaaa ataaacgttg acataaagac tcctgtaaaa aaacgggagc cttcgccccg
    11041 acggacaaaa actcccgtca gggttaaaac accaccagta cagcatggtg ttgctgtcag
    11101 aaaaagcccg ttaataaagg gcgcagttat aaagaaacct tcatcgtcat gactgacgat
    11161 atctgctctc aaaggtgggc agatttacca ccaccagaaa catcctggct gcacatcccc
    11221 tttcaggtta cgggggtgtt gatcacccga aggcgttcct ctcagatcaa cggaacattg
    11281 gtgcccaccg aagcgggatg tggtcacccg aaggcgctcc tctcagacca cttatggagc
    11341 attggtggtc gccgaagcga catataagcc tgtatatttt taaatcacaa aagatgaatg
    11401 tcaagttatt caacatcaat ccaaagagga atattatgta ttcccttaac gggttctccc
    11461 agaggaaaaa cacaggtccc tttaatcagt atttttcttc ccgaccatct gacatcacag
    11521 ttaaaatctc ccaccacatc aaaataatcc gcaatggatt catagatatc ccattcatca
    11581 tagatatccc atttatctgc tgttccttca cattcaaact cggcctgata tccggaatca
    11641 acatatcgcg ccagatagtg tgaccacaaa ggcgaatttt ttgtaatctc ttttacagag
    11701 attattttca gcaaaatagt acaatccata atatttaccc gtacgaaatc gccatgagtt
    11761 attactcata gcctaacaac tgacaataaa cctgttcaat atatcgtcct ctgccatcac
    11821 catgaccaag atcaattgat gtcagtgcaa gtgcctcctt ataactgtaa ccgttcataa
    11881 tatgatattt catggcactt gcagcgtaac tgtaccgaaa actgtgtgca gtctcatctc
    11941 cgcctttcag ttctccataa cgacgtaatg tattaagata ataatccatt gccgacttaa
    12001 gattatctct gtctataaga cgtccattat gttcactggc atatttgagt gcataattaa
    12061 cagcatcaag aacgttatcc cggtcaacaa ccgttgtcat tctttcccgt ccccctttcg
    12121 taccaaaaat cactcttatt ttttcatctc cccgttgtaa cgcttttttc caggttcgta
    12181 atgatttggc cgactggata gcttcttcat ttctcagacc gaggtaacgt gacagtttaa
    12241 caacagcagc caccccttta tcttttgctt ccacacgctg aagaatctcc cgaaaacgct
    12301 catccgtgat taattttttt gttcccttac gactggagcc ggaaatgcct aacgctttat
    12361 tgctgagacg aggatgttcg ggatcagcca tcttatattt acctgcctga cgtaacataa
    12421 cccgtattgc agacatttca ttttcccttg ttcgcccaga aacattactc cggcttctca
    12481 tatatagctc aatatgccgg acttttacct gcgcggcaga actgatctgt acattcattt
    12541 ttgccagact ttcagcaaag cggtgcatga tcctggtgta atcactcagc ttcttgaagc
    12601 tgcctttgtc ttttcgaatg tgagcaacca gatcctgttt cagtaatttt tcacgtctgg
    12661 acatcaactt ctcctgcaca taataatttt taccttaaac agctgtacgt tcggcagatg
    12721 agtttttctg ttgtgccgtg aacacaacag aaaaactcat ctgcatttct ccctgacgac
    12781 acggttaaac gttgcgatcc ttcgggtagt gactgcctgt gcgccagggt agcgcagatt
    12841 gtttaggtat tgaggggtaa caaatcggtg ttgcacaccg actgcagcat ttgctgtacc
    12901 tgtctccagg tcgcccccga caccacctct aataagtggt atcgccgatt cgacatctca
    12961 ctacataaaa ctccttaaaa aacaattgat tctgcaaaaa accatttatg gacgttattc
    13021 acaaaatcgt ccgtaaattt gcagaagagg ttgaaaaaac actctcacgc cgggtcactt
    13081 cttcaaatgt taaaaaacat cacgtgaaac ggcagttcgt gcgggttcgt taagcagacg
    13141 cgcgtgcgtc actttgtcat cgtgacacgc tgacgaaagt gacgccgcgc agcttcagac
    13201 gccagataca cgcgtaagcg accaaatttc aaatggtctt atacagcgcg cacagtcgca
    13261 ggaagtgctg gcaaacaaat gaaaagaata aaagaagtca ataccgccat tacgcagtaa
    13321 gaacattccg gcatcaccgg atggataagg atcacacaac cactgctgtg tattgcccgt
    13381 aataaagggc gcggttaaaa gaaaccttca tcgtcatgac tgacgatatc tgctctcaaa
    13441 ggtgggcaga tttaccacca ccagaaacat cctggctgca catccccttt caggttacgg
    13501 gggtgttgat cacccgaagg cgttcctctc agatcaacgg aacattggtg cccaccgaag
    13561 cgggatgtgg tcacccgaag gcgctcctct cagaccactt atggagcatt ggtggtcgcc
    13621 gaagcgacag ataatgcatt ctgagcatat cagagcgcat aaaaggcgtc aattatccat
    13681 gctccctcac gaaacagaag ctgaactctt aacacctgct atcggaactc actgaataaa
    13741 caactaaaaa ggacattgtg aaaattaaca tcaacagtat gtaaagtgat ttgttttgtc
    13801 ttaaatgggt gaatgtaaaa aaatattcta tacaatccac acatccttgt atcatatatc
    13861 aagtaaacat ccacagagat aacagcttat ttcaaataag cagattctgt ttgatacttc
    13921 agaaaaata
//
LOCUS       DAZKHK010000125        13868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKHK010000125 DAZKHK010000000
VERSION     DAZKHK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.125"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..1236
                     /locus_tag="ACHOS5_004000"
     CDS             94..1236
                     /locus_tag="ACHOS5_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFP9778739.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLMD"
     gene            1709..1810
                     /gene="ykgR"
                     /locus_tag="ACHOS5_004001"
     CDS             1709..1810
                     /gene="ykgR"
                     /locus_tag="ACHOS5_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP9778740.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2175..2438
                     /gene="ykgM"
                     /locus_tag="ACHOS5_004002"
     CDS             2175..2438
                     /gene="ykgM"
                     /locus_tag="ACHOS5_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9778741.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            2438..2578
                     /gene="ykgO"
                     /locus_tag="ACHOS5_004003"
     CDS             2438..2578
                     /gene="ykgO"
                     /locus_tag="ACHOS5_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP9778742.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(2613..2838)
                     /gene="ykgL"
                     /locus_tag="ACHOS5_004004"
                     /pseudo
     CDS             complement(2613..2838)
                     /gene="ykgL"
                     /locus_tag="ACHOS5_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            3662..4204
                     /gene="ecpR"
                     /locus_tag="ACHOS5_004005"
                     /gene_synonym="matA"
     CDS             3662..4204
                     /gene="ecpR"
                     /locus_tag="ACHOS5_004005"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP9778743.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            4279..4866
                     /gene="ecpA"
                     /locus_tag="ACHOS5_004006"
                     /gene_synonym="matB"
     CDS             4279..4866
                     /gene="ecpA"
                     /locus_tag="ACHOS5_004006"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP9778744.1"
                     /translation="MKKKVLAIALVTVFTGTGIAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            4923..5591
                     /gene="ecpB"
                     /locus_tag="ACHOS5_004007"
                     /gene_synonym="yagY"
     CDS             4923..5591
                     /gene="ecpB"
                     /locus_tag="ACHOS5_004007"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP9778745.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            5617..8142
                     /gene="ecpC"
                     /locus_tag="ACHOS5_004008"
                     /gene_synonym="yagX"
     CDS             5617..8142
                     /gene="ecpC"
                     /locus_tag="ACHOS5_004008"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP9778746.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLTTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            8132..9775
                     /gene="ecpD"
                     /locus_tag="ACHOS5_004009"
     CDS             8132..9775
                     /gene="ecpD"
                     /locus_tag="ACHOS5_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP9778747.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            9744..10454
                     /gene="ecpE"
                     /locus_tag="ACHOS5_004010"
     CDS             9744..10454
                     /gene="ecpE"
                     /locus_tag="ACHOS5_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP9778748.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPSGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            10761..11090
                     /gene="ykgJ"
                     /locus_tag="ACHOS5_004011"
     CDS             10761..11090
                     /gene="ykgJ"
                     /locus_tag="ACHOS5_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9778749.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGQNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(11102..11268)
                     /locus_tag="ACHOS5_004012"
                     /pseudo
     CDS             complement(11102..11268)
                     /locus_tag="ACHOS5_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(11339..11953)
                     /gene="yagU"
                     /locus_tag="ACHOS5_004013"
     CDS             complement(11339..11953)
                     /gene="yagU"
                     /locus_tag="ACHOS5_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP9778750.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(12216..12353)
                     /locus_tag="ACHOS5_004014"
     CDS             complement(12216..12353)
                     /locus_tag="ACHOS5_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778751.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     A"
     gene            12402..12560
                     /locus_tag="ACHOS5_004015"
                     /pseudo
     CDS             12402..12560
                     /locus_tag="ACHOS5_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(12777..13470)
                     /locus_tag="ACHOS5_004016"
                     /pseudo
     CDS             complement(12777..13470)
                     /locus_tag="ACHOS5_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         3898 a         3313 c         3205 g         3452 t
ORIGIN      
        1 gaattctatt aaaggaaaaa agcatcaaaa agtagcaact caacttatta agattatacg
       61 cattaaaatc agataaacat ttgaggtatt gttatgacaa ataaacaccc atcattgttt
      121 tctcctttta tgctgacaga aaaaataaag ctacgtaatc gtatcgttat ggctcctatg
      181 acaacctggt cagctaatcc tgatggaacc atctctgagc aagaactcga attttataaa
      241 cgtcgctcac aaaatgttgg tttagtaata acaggatgta cctatgtcac gccaagcggc
      301 attgggttta ctcatgaatt tgccgcgtat gacgaccgtt tcattaacag ccttgaaaag
      361 ttagcagctg cagccaaaag tggcggtgct ccggctattt tgcagatttt ccacgccggt
      421 aataaagcca tcccggagct tgtgccaaat aacgatgtta ttagtgccag cgcatcaagc
      481 gtaaaatctg gtgactttat gaagagagtg gtacaaagca gggaaatgac tgaaaacgaa
      541 attcaggaaa ccattagagc attcggagat gtcacaaaaa gggcgattaa agcaggtttt
      601 gatggcgttg aacttcatgg tgcgcacgga ttccttctgc agaatttctt ctcccctcta
      661 tttaaccagc gtaatgaccg atggggagga gatcttgaag gacgtatgcg cttcccgctg
      721 gccgtattac aggaagtaaa aaatgtagtc tatgaatatg cgacaaagcc ctttgctatc
      781 ggttacagaa tatctcctga agaatctgta acaggggggc tgcggataga ggatacttat
      841 aaactccttg atcggctcat ttcatccggt atttcgtata tacatacctc gctggtcagt
      901 attaacgata gctaccctgt tgagtcgcca aatggccccc gaactattga attgatttta
      961 aatcatattg caggcagagt gccggtaatc gccgcaggta aaatacgcac accgtctcag
     1021 gctcaagaag caatttccgc tggcctgcca cttgttgcga tcggtaaagg gctggtgata
     1081 aatccagaat gggtgacttt agctgaatca ggtagaagtc atgaaattca aacagccctt
     1141 aatccacaac gcgttcctga attaaccatt ccggataaac tatgggatca aattcaggca
     1201 tcgaaaggaa ctggctggtt tccgttaatg gattaaatat cgattactct cccgagagta
     1261 aaactatcaa atcaatgagt aaccgataca gcctcgcaaa ttctttcaga taattaaccc
     1321 gtcactgcgg cacgttctct cgtaaggcgt gcctgcagtg taatccagta tgtatccggc
     1381 atagttgacg gtttaataga ctcaattcct ctccctggtt acctacaacg atatcttgta
     1441 taacacccca ggagatacga cgttacgatg agatgcgata tgctcctcag cgccaatcaa
     1501 cgccaggact gggccagtct tgagatcaga ctcattcccg taactgattt caggcgacat
     1561 atatcaatga cattggggca ataaaagata gccgatgtaa tacatctatt caaatttgtg
     1621 atacatgtca aaaaattggt tgaccaaact cgttatttat ataagggcac ttacgaagtg
     1681 cactcttttt taaagcgagg aagtaccaat gaaagagaat aaagtacagc aaatcagtca
     1741 taaactgatt aatatcgttg tttttgtcgc aattgtagaa tacgcctatt tatttctcca
     1801 tttctattaa taacggcaat aaactgttca cttcagtgat atttaaaata tgcatcctct
     1861 cccttttttg taagtaatta ttatatccgt gggagaggaa tacacattgt caggtaatca
     1921 atcatgctgc aataaatcat cggccagtaa agtggagata gcctccattc tcgaaaaatc
     1981 catactctca gcgaaaccat catcaatcac tcatccaggc gtttatggga gcgtcgccaa
     2041 tggctgctaa caatgccaga cttccccgtt gcggaaattc cacatcccac aaatagtcac
     2101 agtgattggg tgttgaaatg atccggatga gcatgtatct ttacggttat gttataacat
     2161 aacaggtaaa aatgatgaaa cccaatatcc atcctgagta tcgtactgtg gtgttccacg
     2221 acaccagcgt tgatgagtac tttaaaatcg gctcgactat caaaacagac cgtgagattg
     2281 agctggatgg cgtaacgtat ccgtacgtaa caattgatgt ctcttcgaaa tcgcacccgt
     2341 tctatacagg gaagctgaga acagtggcat cagaaggaaa tgttgcacgg ttcacccaac
     2401 gttttggtcg ttttgttagc acgaaaaagg gggcatgatg aaagtcctta actctctgcg
     2461 taccgcaaaa gaacgccatc cagactgtca gattgtgaag cgaaaaggac ggctgtatgt
     2521 gatttgtaaa tctaatccac gttttaaggc cgttcagggt cgtaagaaaa aacgttgatt
     2581 taaaattcga cgaattaacg atatttgtct gattaataat cagaccggat taatgttggt
     2641 gtatttatta caccagcatt aattttcctg gggatgtatc ttcctgttca tttaaggtca
     2701 actgcctgac gtttctctcc gaatattcca ttatattaat gttgacttgt tgaccagctg
     2761 cgccctgtat gctggcatca actctatttt agactgaaca cgccactcag tttcctccct
     2821 ttgcgacgca gcctgcattt acactcaaac tgttaagatg ataaatgtgg taaatctgtt
     2881 ggtactgaca taaaaacgtt tacgccacag gaacggccag atccatcggt aaccccatcg
     2941 ccgacgttcg agtgccagtt agagtaacgc gtacagatga ctgaatgcag tgccctggca
     3001 aagaggccat cgttcctgtg acaactggca gtcttcgttt aacttcactt aatttggctc
     3061 ttggggggct taccggacag atgacgtact tacacctgtt taatttttca tcacttattg
     3121 ggatgaacac ccataaccat tttgtgcggc atgttaatcc attaaaacac cttactgatt
     3181 ggcaaatcat ctttaattat gacttatgat agttttatat tctatttctt atcatttaaa
     3241 cttgtttttt tactagtcca ttacacaaca cattaagact attcctaaca cctcagggca
     3301 aagttcctgg ctaatataaa atgcaagtaa gaattgaacg ttatattgcc aataacctta
     3361 tgaaactgaa tgtctttttc ttcttatcaa aaaagcaata ttttcatttt ttgtaaatat
     3421 tgacttaacc atggaattca ttttctgttc acatattgac actcatcagg aaaaaaacat
     3481 aaatttaaac ctaatcgaaa taattaaaac ttaatctcgt ttaacctata ttgatatgtg
     3541 ctacgtatct tatttacttc cgatttacta aagaaactga atgtacctgt aaaaattaca
     3601 ggtttggaaa gtagtgacat ggcaaaatga ttacagcagg gactatgagg ttaaaaacca
     3661 tatggaatgt caaaaccgtt ctgataaata catctggtct ccccatgacg cctacttcta
     3721 taaaggacta tctgaactga ttgtggatat cgacagatta atttatctat cgctggagaa
     3781 aatcagaaaa gatttcgtgt ttatcaatct caatacggat tctttaactg agtttataaa
     3841 ccgtgataat gagtggttat ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag
     3901 aaagtcagaa gccttagcaa attattggta ttacaacagc aatattaggg gcgtggtata
     3961 cgctggactg agtcgtgata ttagaaaaga actggcctat gtgattaatg gcaggttcct
     4021 gagaaaagat attaagaaag ataaaatcac tgaccgggaa atggaaatta tccgcatgac
     4081 ggctcaggga atgctgccta aatcgattgc cagaattgaa aattgtagtg tgaagacagt
     4141 gtatacccat cggcggaatg cagaggccaa gctgtactca aaattatata agttggttca
     4201 gtaaactcca ggcaagttag ttttaaaaaa tgactcaccg ggacttcatg tcctcaattc
     4261 aactcgggaa gaaaagcaat gaaaaaaaag gttctggcaa tagctctggt aacggtgttt
     4321 accggtacag gtatagcgca ggctgctgac gtaacagctc aggctgtagc gacctggtca
     4381 gcaacagcca aaaaagacac caccagtaag ctggttgtga cgccactcgg tagcctggcg
     4441 ttccagtatg ccgaaggcat taaaggtttt aactcacaaa aaggtctatt tgacgtggct
     4501 atcgagggtg actcaacggc taccgccttt aaactgacct cacgccttat caccaacacc
     4561 ttaacccagt tggatacctc aggttccaca ctgaatgtgg gcgtggatta taacggcgcg
     4621 gcagtcgaaa aaactggcga taccgtgatg atcgataccg ccaacggcgt actgggcggc
     4681 aaccttagcc cactggctaa cggttacaat gccagcaatc gtaccaccgc acaggatggt
     4741 ttcactttct ccatcatcag cggcaccacc aatggtacca ccgcagtaac cgattacagc
     4801 actctaccgg aaggcatctg gagcggcgac gttagcgtac agttcgacgc tacctggacc
     4861 agttaatctc tctgatgtac cagcaggggt acccccctgc tttattcctg gacggaattg
     4921 ttatgaaaaa gcaccttctg cctctcgctc tgctgttttc cggaatatca ccggcccagg
     4981 cgctggatgt cggcgatata tcatcgttta tgaacagtga cagcagcacg ctgagcaaaa
     5041 cgatccaaaa cagtaccgac agtggccgcc tcatcaatat ccgtctcgaa cggctctctt
     5101 caccgcttga cgacgggcag gttatcgcaa tggacaagcc ggatgagttg ctactcactc
     5161 ccgccagctt gctgctaccc gcccaagcca gcgaagtgat ccgcttcttc tataagggac
     5221 cagcagatga aaaagagcgc tactaccgca ttgtctggtt tgatcaggcc ctcagtgatg
     5281 cgcagcgcga taatgccaac cgtagcgctg tggccactgc ttccgcccgc atcggcacca
     5341 ttctggtcgt cgcaccccgc caggcgaact accactttca gtacgccaac ggctccctga
     5401 caaatacagg aaatgcgacg ttgcggatcc tcgcctacgg cccctgcctg aaagccgcca
     5461 atggtaagga gtgtaaagag aattactacc tgatgccggg caagtcgcgt cgttttaccc
     5521 gcgtggacac ggcggataac aaaggacggg ttgcactttg gcagggtgat aagttcattc
     5581 ccgtgaaata gatagctgtg cagatggata acgacaatgc ctttacgacg gttctcccca
     5641 ggactgaaag cccagtttgc cttcggcatg gtctttttgt tcgttcagcc cgatgccagc
     5701 gctgctgaca taagtgcgca gcaaataggt ggggtgatta ttccgcaggc cttcagtcag
     5761 gcgcttcagg acggcatgag cgtcccgctc tatattcatc tcgccggtag ccagggtcgc
     5821 caggacgatc agcgaatcgg cagcgctttt atctggttgg atgatggaca gttacgcatc
     5881 cgaaaaatac agctggaaga gagtgaagat aacgccagtg tcagcgaaca aactcgacag
     5941 cagctgacga ctctggccaa cgccccgttc aatgaggccc ttaccatccc cctgactgac
     6001 aacgcgcagt tggatctcag cttgcgccaa ctgctgctgc agctggtggt caagcgcgaa
     6061 gcgctgggca ccgtactacg ctcacgtagc gaagacatcg ggcagtccag tgttaacacc
     6121 ctcagcagta atctgagcta taacttcggc gtctataaca accagttgcg taacggcggg
     6181 agcaacacct ccagctatct gtcgctgaat aacgttactg cgctgcgcga acatcacgtg
     6241 gtgctcgacg gctcactgta cgggattggt agcggtcaac aggacagtga attatataaa
     6301 gcgatgtatg aacgcgattt tgctggtcac cgatttgccg gtggaatgct cgacacctgg
     6361 aacttgcagt ccttagggcc gatgaccgcc atttcagcag ggaagattta cggcctttcc
     6421 tggggaaacc aggccagctc caccatcttc gacagcagcc aatcggccac gccagtgatc
     6481 gcctttttac cggcggcggg cgaagtacat ctcacccgtg atgggcgact actaagcgtt
     6541 cagaacttca ccatgggcaa tcatgaagtg gatacccggg gtctaccgta cggtatttac
     6601 gatgtggaag ttgaggtaat cgttaacggt cgcgtgatca gcaaacgcac ccagcgggtc
     6661 aataagctgt ttagccgggg acgtggcgtc ggtgcaccgc tggcgtggca gatatggggc
     6721 ggtagctttc atatggatcg ctggtcggaa aacgggaaaa agacgcgacc agccaaagag
     6781 agttggctgg caggtgcctc gacctccggc tcactgagta cgtttagctg ggcggcaacg
     6841 ggatatggat acgataatca ggcggtgggt gaaacccgtc tgacgctgcc gcttgggggg
     6901 gcgatcaacg ttaacctgca aaacatgctg gccagtgaca gctcatggag caacatcgcc
     6961 agcatcagcg ccactctacc tggaggcttc agttcgctgt gggttaacca ggaaaaaacc
     7021 cgcattggca atcaattgcg acgtagcgat gccgacaacc gtgcaatcgg cggcacactc
     7081 aacctgaact cactgtggtc gaagctgggt acgttcagca tcagctacaa tgatgaccgc
     7141 cgctacaaca gccattatta cacggcagat tactatcaaa gtgtctacag cggtaccttt
     7201 ggttcgcttg gcctgcgggc cggtattcag cgctataaca acggcgacag cagcgccaat
     7261 acagggaaat atatcgctct cgatctctcg ctaccactgg gcaactggtt tagcgcaggg
     7321 atgacccatc aaaacggcta caccatggca aacctgtcag cacgcaaaca gtttgatgaa
     7381 ggaaccattc gcactgttgg tgccaatctg tcacgagcca tctccggcga taccggtgat
     7441 gacaaaaccc tcagcggtgg ggcgtatgca cagttcgacg ctcgttacgc cagcggaacg
     7501 ctgaacgtca atagcgcggc ggacggctac atcaatacca atttgaccgc caacggcagc
     7561 gtcggctggc agggtaaaaa catcgctgcc agcgggcgga ccgatggcaa cgctggggtg
     7621 atattcgaca ctggcctgga gaacgatggt cagatcagcg ccaaaatcaa cgggcggatt
     7681 ttcccgctta acggcaagcg taactatctc ccgctctctc cctatggaag atatgaggtg
     7741 gagttgcaga acagcaaaaa ctcactcgac agttacgata tcgtcagcgg tcgcaaaagt
     7801 catctgactc tctatccagg caatgtcgct gtcattgagc cagaggtgaa gcagatggtt
     7861 accgtctccg gtcgtatccg tgcggaagac ggcacactac tggctaacgc acggattaac
     7921 aaccatatcg gccgaacccg aaccgatgaa aacggcgagt ttgtcatgga cgtggataag
     7981 aaatacccca ctatcgattt tcgctacagt ggcaataaaa cctgcgaagt ggcactggaa
     8041 ctcaaccagg cgcgcggtgc cgtctgggtc ggtgatgtgg tctgcagcgg cctctcatcg
     8101 tgggcggcgg tgacgcagac aggagaagag aatgagagtt aacctactga tagcgatgat
     8161 aatctttgcg ctaatctggc ctgcaaccgc gctcagagcg gcagtgagca aaacaacctg
     8221 ggcggatgct ccggcacgcg agtttgtgtt tgtcgaaaac aactcagacg acaacttttt
     8281 cgtcactcct ggcggggcgc tggatccgcg cctgaccggt gccaaccgct ggaccggttt
     8341 aaaatacaat ggttcaggaa ccatctatca gcaaagcctc ggctacattg ataacggtta
     8401 caacaccggc ctttatacca actggaagtt tgatatgtgg ctggaaaatt caccagtttc
     8461 atctccttta actggcttgc gctgcatcaa ctggtacgca gggtgtaata tgaccaccag
     8521 tcttatcctg ccgcaaacca ccgacgccag tggattttat ggcgcgacgg tgaccagcgg
     8581 cggcgcgaaa tggatgcacg gcatgttgtc agacgcgttt taccagtatc tgcaacaaat
     8641 gcccgtcggc agcagcttta caatgaccat caatgcctgc cagacctctg tgaactatga
     8701 cgccagcagc ggcgcacgct gtaaggatca ggcctccggc aactggtatg ttcgcaacgt
     8761 cacccatacg aaagcagcaa atctgcggtt gataaatacc cactcgctgg cggaagtatt
     8821 tatcaacagc gacggagtac cgactctggg cgaagggaat gccgactgcc ggacgcaaac
     8881 catcggcagc cgttcaggat taagttgtaa gatggttaac tataccctgc aaacgaacgg
     8941 actcagcaac acctcaatcc atatattccc ggcgatcgcc aactcgtcgt tagcctcggc
     9001 cgtcggggcg tacgatatgc agttcagtct gaatggcagt tcatggaaac cggtgagcaa
     9061 tactgcctat tactacacct tcaacgagat gaagagcgca gactcgatct atgtgttctt
     9121 ctcgagcaac ttctttaagc agatggtgaa cctcgggatc agcgatatca acaccaaaga
     9181 tctattcaac tttcgctttc agaacaccac atcaccggag tctggctggt atgaattttc
     9241 tacctccaac acgctgatta tcaaaccccg tgatttcagc atcagtatta tctccgatga
     9301 atatactcag acaccgtcgc gggagggata tgttggcagc ggcgagtcgg cactcgattt
     9361 cggctatatc gtaaccacca gcggtaaaac agctgccgac gaagtgctga tcaaggtgac
     9421 cggacccgcg caggtgattg gcgggcgctc ctattgtgtc ttcagctccg atgacggtaa
     9481 ggcgaaagta ccgttcccgg cgacactttc ctttattacc cgcaacggag ctacaaaaac
     9541 ctacgatgcc gggtgcgatg atagctggcg ggatatgacc gatgcgctgt ggttgaccac
     9601 accgtggact gatatctctg gcgaagtggg gcagatggat aagaccacag tcaaattttc
     9661 gattccaatg gataacgcca tttctctgcg tacggtagat gataacggct ggtttggcga
     9721 agtcagcgct tcaggagaga ttcatgttca ggcgacgtgg cgtaacatta actaaggccc
     9781 tgctgacagt ggtctgtatg ctggcggcac ctttgacaca ggcgatttcg gtcggcaatc
     9841 tgactttttc actgccgtcc gagactgact ttgtcagcaa acgtgtcgtg aataacaaca
     9901 aaagcgcacg gatataccgt attgccatca gtgctattga tagcccgggc agcagtgaat
     9961 tgcgcacccg accggtggat ggtgaactac ttttcgcccc ccgccagctg gcgttgcagg
    10021 ctggtgagag cgagtatttt aaattttact atcatggtcc acgggataac cgcgagcgct
    10081 actaccgggt ctcatttcgc gaggtcccca ctcgtaacct gacaaaacgc agcccaagcg
    10141 gcggtgaggt cagcacggag ccggtggtgg tgatggatac cattctggta gtacgaccac
    10201 gtcaggttca gtttaaatgg tccttcgatc aggtgaccgg aacggtaagt aacaccggca
    10261 acacgtggtt taagctactg attaaaccag gatgtgattc gaccgaagag gaaggcgatg
    10321 cctggtatct acgtccggga gacgtggttc atcagcctga gttacgtcag ccggggaatc
    10381 actatctggt ctataacgac aaattcatta agattagcga ttcctgtccg gctaagcccc
    10441 cttcggcgga ctaagactta tccatcggac gagggaagaa ttccacctca gagactcaaa
    10501 aatcgtttaa atgatgtgga agcgatcgtc aatgtcaccg atgagctttc tcatatgcac
    10561 accacagtgt tcaggagaca cggaaaggta ccaggcggcc tgctaacgag tggtttcttc
    10621 actaattagg acgtaaaatc ggtaacggct ggaaatcatt caatactcac actatcgaaa
    10681 gtacaccagc caaccgcgat acgtccctgc atacgacgta ccgcagttct ctttacgatt
    10741 tatagctgta ctggtgaatt atgagcaatc tgaatccatg catgacgtgt ggtgcctgtt
    10801 gtgcattttt ccgcgtctct ttttactggg ccgaagccga cgatgctggc ggtaccattc
    10861 ccgccaggct cactgaacaa atatcccctt ttcaccgatg catgagcggt actaatcaga
    10921 aaaatccccg atgtattgcc cttgcaggaa caccgggcca aaatgcctgc tgcacgatat
    10981 atgaaaatcg atcgtccaca tgcagagaat tcgccatgtc tggtgagaac ggagaagtca
    11041 atgaggcttg caatcgtgca agagctaaat acgggctaac accgttataa atatacaata
    11101 attaattgca ttgccccgcc agctgtaata gcggggcttc gtttttcagg gacaacaaaa
    11161 cctgaaactt cttccatttt ccagcaacaa tgcctcttct tctgtagcgt tctccggatc
    11221 tacatcatcc tgaagtagcg cgatatgatc actgactgtc cgcatcagaa tatgaaaagc
    11281 aacgttttta acctataaaa atggcgctat atttgcgcca tttttatcat tcaaagcatt
    11341 atctatttga gcctaaaggg atctcaggat ctggctcatg ggtaattctg tttcgtaaat
    11401 ctctgcgaat aatttcaata gaccagaacc agactaaatg tccaaaaatt tcagagacat
    11461 tctcatacca cggaagatca aagagaggtg gtgtaagtcc catgagaggg aatgaaatca
    11521 tatgaacaaa aagttgcgct aaagcacctg ccagtaagcc ctgccaaagt ttaattttcg
    11581 gaaatacttc ggcgaccaca cagtaaccaa cggcaaaaac tatcgaaaag attatgtgcg
    11641 ttacaccaac ccagttaaag acatgcccgg cgaaggtata aacagccgaa tttggatctg
    11701 tcagtcccaa ccaatcccga agaaaaatgt acggaggatt gagaaagtta cgggagcaat
    11761 caatttggcc tgcagccctg attaatgatt ccggaccaca cgctgcatta aacatatcaa
    11821 ccggactacg tggtggcaat ggaacttctg ccccccactt aacaaatgcg gaaacaacgc
    11881 cagcaatcag cccaatgaat gcagcaagac cataacgtct gcggtttggt ggagtttgtt
    11941 caaatatatt catatctacc ctgcttgtac cattatgtta tacacctctt taggagtatt
    12001 cataaaacaa ggcaaatgta aagaactgta ttgttttgta taaccacatg gttacctaat
    12061 cgccaattaa tataagctcc attatttctc catattttta tgttagaagt gacaaggaga
    12121 aatgtaatgt ttctctcaga gaatataaat cattcctttt gcccacctta atggtgggct
    12181 tttttatgca aaacctgaac gtcctgtggt attcctcagg cataatgatt ttcgcgatta
    12241 accaacaacc agaccagcaa agacgccacc acaagcacag taacatccat caggcttgcc
    12301 acaccccatg cgtgcggctc aaagccgcca tggcatatta atccgcttgc cattttcgaa
    12361 cagctgattg gtttggtccg gtgtttccct gcttgcactt aaaaatgagc ggataggaaa
    12421 ctctcgaaat ggttcgtcga tatgctcatc tcgcacctaa tcaccttacc gaacacgcac
    12481 ggtaaataga ttcgatcctg aacccatcgg tcccaaatct gtcccaatca aaaaataagg
    12541 aaggtattaa cgatgtgtaa cctattgatt taaaatggat aatataaaca gattgtaaaa
    12601 agcaagacat tgatatgtat ataaaaaaac tattacgcaa tcattagata cccccagatt
    12661 tagttgtaaa gcaagcacac tttgcatttc gagacaatcc aggtaaagac tcttacctcc
    12721 cattttgtga tttttatact tatgatgata agttgaacgt tgggtagtgc ttccaatcag
    12781 tagaacatgt gcttttcaat gaaggaaccg atgacttttt cgtggattgc aacggaacgc
    12841 gagaagcaga ttgttttact taccagccgc ttgatgcggg tgcggagggt caggttgtta
    12901 cgttcaatac gttgcgtgaa gattttgccg gtcaggtatt tctccttcgg cacttctctg
    12961 gcgtagctgc cccagtcatc gcggccacca tgccgataca gaacggagtc aacagggcta
    13021 acaattcccg acaggcctca tcattccgtg gcccgaaagt gtaagccaac acaccgccag
    13081 ttttagtgtt ctacgcatac cagagccagt gttgttgcgc cttgttgcca acgaagctcc
    13141 acagctcatc aagttcacag ataagggcaa catcagcgtg agctgactga cgcagatgtt
    13201 atccgctggg gcgagagctt tttaatgtcc gtatgacggt attgatgctg attttcagtg
    13261 ttctggtgat atcacggccc ctgtgccatt atgcgccatt tcaacgatca gctccttaaa
    13321 gccgggtttt ctggcttcat aagagtaagt gagctggaaa acacgtttac atgaacgaca
    13381 acggaaacgc tcatgctggg aacaactacg accatgtcga taaactttat cggaattaca
    13441 gcgcggacaa tgaacagtaa cggtagccat ggaaaacctt aaataccagt agattaccag
    13501 aaacttctat caattgaaga cattaccgtt tttgattaca gcaaatgttt tattaattac
    13561 cccaccatcg actaaatccg ttagaacgtc gaatgatgcc attcgtaaat acattaaaaa
    13621 gactgttgta atcaacgaag atggaaaaca gtgtgttaaa actaatctga acttcagaaa
    13681 aacagatgat ggtatggtga tatatatatc cgctctgaac cgccacgcaa tttaaaataa
    13741 aaatcaacaa atatcgtcga tatccatata taccattttt ctgtagttgt attagcgtcg
    13801 catattttat gactttaagt cataagtcaa aacacctgag aaaaagcaaa caacattttc
    13861 attaccac
//
LOCUS       DAZKHK010000126        13809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKHK010000126 DAZKHK010000000
VERSION     DAZKHK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.126"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(3..3125)
                     /gene="agn43"
                     /locus_tag="ACHOS5_004017"
                     /gene_synonym="flu"
     CDS             complement(3..3125)
                     /gene="agn43"
                     /locus_tag="ACHOS5_004017"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP9778752.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNVGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNNTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQSN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIVRGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDSSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNHDFRARGRGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYVHSVSGSSAEGY
                     NGQATLNVTF"
     gene            complement(3498..4370)
                     /locus_tag="ACHOS5_004018"
     CDS             complement(3498..4370)
                     /locus_tag="ACHOS5_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9778753.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHTGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            5600..7084
                     /locus_tag="ACHOS5_004019"
     CDS             5600..7084
                     /locus_tag="ACHOS5_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9778754.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PNESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(7406..8008)
                     /locus_tag="ACHOS5_004020"
     CDS             complement(7406..8008)
                     /locus_tag="ACHOS5_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778755.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(8095..8301)
                     /locus_tag="ACHOS5_004021"
     CDS             complement(8095..8301)
                     /locus_tag="ACHOS5_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9778756.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            9060..9209
                     /locus_tag="ACHOS5_004022"
     CDS             9060..9209
                     /locus_tag="ACHOS5_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP9778757.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            complement(9555..9752)
                     /locus_tag="ACHOS5_004023"
     CDS             complement(9555..9752)
                     /locus_tag="ACHOS5_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778758.1"
                     /translation="MVIRKTVCRDCDNAIPHNTECCPGYVSADPFDYYRNTDRLLCLL
                     TLLPVLILAVVSGVSVFVLLQ"
     gene            9957..10526
                     /locus_tag="ACHOS5_004024"
     CDS             9957..10526
                     /locus_tag="ACHOS5_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP9778759.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGIIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            10786..11163
                     /locus_tag="ACHOS5_004025"
                     /pseudo
     CDS             10786..11163
                     /locus_tag="ACHOS5_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            11167..11592
                     /locus_tag="ACHOS5_004026"
     CDS             11167..11592
                     /locus_tag="ACHOS5_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9778760.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLSVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
     gene            11839..12036
                     /locus_tag="ACHOS5_004027"
     CDS             11839..12036
                     /locus_tag="ACHOS5_004027"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9778761.1"
                     /translation="MPQDDFCIVNPGGCIVHFPANGRDIQDINAPDFPEQYQRLLARL
                     DYLTKPDAKASGQRNSGYSGF"
     gene            12064..12630
                     /locus_tag="ACHOS5_004028"
     CDS             12064..12630
                     /locus_tag="ACHOS5_004028"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9778762.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESYGAIERHLGCSDF"
     gene            12686..12950
                     /locus_tag="ACHOS5_004029"
                     /pseudo
     CDS             12686..12950
                     /locus_tag="ACHOS5_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991605.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13016..13213
                     /locus_tag="ACHOS5_004030"
     CDS             13016..13213
                     /locus_tag="ACHOS5_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9778763.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFIGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
BASE COUNT         3244 a         3398 c         3624 g         3543 t
ORIGIN      
        1 tgtcagaagg tcacattcag cgtggcctga ccgttatagc cttcagcgct gctgccgctg
       61 acgctgtgga cataaccagc ctgaacgccc agggtgatgt tttcccggac acgggcttcc
      121 agtccggcct gcaggtccag tgacgtacca ttctgtgacg gtgagaacgt catgttactg
      181 ccggctgcgg ctgtacccat gctcatgtcc ccccgggaac tgaaggtgcg gataacagaa
      241 ggctgtaccc accagttcac cggcagttca cgcacactgt gtttggcact gccgcgcagg
      301 gtgtcacggg atgaggtgcc tttaccaaag ttcatatcgt tgtggctgcc cagacgaaat
      361 ccggcacgca catgttgtgc actgccatgc ccgaacttca cataaccggc gttgtcctta
      421 ccgtcatcca gggaaagtcc ctgccaggtg tactgcagtt gtggctccag catcaggttg
      481 tcagtgatac tgaagggcag accggtttcc agtgagccca gccagccccg gccccgtgcg
      541 cggaagtcgt gattgtccga tgaggctttc atgctgtggc gggttccctg ggccacaatg
      601 tcagcccaca gtccggaggc gttgtgtatc agattcaggt atccgcccag gctgccggca
      661 tcatcccgga ccgtgccggc acgggagctg tcatcatcct taacatcaac ggaagaatgg
      721 cctgcagcac catacacccc tgtcgtcaga gacataccgg cgacctctgt tctgagcagg
      781 tcaccctcaa gacggacgaa gccatagctg ccgctgcttt ccggcgtggc cccacggaca
      841 ataccgccgt tgttatcgtg accgagatgg ccgccctgaa tgctgagacg gacgctgttg
      901 ttttcaccat ttacaccggt ctgatggctg cgggagcctg ccagaatccg gtcatagtcc
      961 attgcctgtg tcagcatgga ggcatacagg gggacttctg cacgataagc attttcactg
     1021 cgcagatacc agctctcatc actgtcccgg ttgagggagt agttaaaggc cccggcctgc
     1081 agcctgttcc cctggacaaa ggccccttcc tccgtggtgg caccgttaat ggcttccacc
     1141 acctgaatac ccttaccgct ggtcgccagc cccgacgcac tgttgccggc gttcaccagg
     1201 ttcaggatgg tttttccggt tgccctgccg ccgtcaatga ccagtctgtc agcattgctc
     1261 tgtgccatat ccgggcgtac acgcaggctg atggtgccat tctgcccgtt caggtttttc
     1321 actttcaggg ttgccggtac gaacttccct gtgcgggtgg aggtgaaatg aatctgtccg
     1381 gcatggctga ggtcatccac caccgactgc accgtggcgt tatcagggat attccaggtg
     1441 gcaccggagg cgagagtgac attcgtgggg tcaatggcac cgttcagcac agtgctgccg
     1501 gtcagcttca gggctgtacc gcgctgagca atgacatccg tggtgacggt actgtcgttc
     1561 agcgtcagcg tgccttcatt caggttgacg gctttctggg tgagtgtggt gttgctgaca
     1621 gtgagtgtgc cacttcctga tttttccacg ctgccgttac cggtgagaga gcctccctgc
     1681 aggagtgcat cgccttcacg gatggtcagg gtattgttgt tcaccgtctt cccggaaagg
     1741 gtaagtatgg cgccgttatt cagcgtggtg gtgcccgcca gtgtgccgcc cctggcggtg
     1801 aacagtccgc catttaccgt ggtatccgtg gccgtatcac cgacgttcag cgtgaatgaa
     1861 ctgccttttt ccagcatcag ggcatccgcc tgaccgcctc cgatactgaa tgcctttccg
     1921 tcgctccgtg taccactgac agcggcaccg gaatcggcca gcagtacacc gccatttccc
     1981 atggatacgg tggtggcggt accaccgttg cggacatcca gcgttccgcc atcatccacg
     2041 cgggtattag tggctgtgtg tccggtcagc acatccaggc gtccgccatt ttccagtacg
     2101 acattatcag ctttaccctc cacaaccgag aatgctccaa ggcggtttat gccggtaacg
     2161 gttgcagccg tactggtaac cagtgcaccg cccgtgttct gggtgactgc agtggcttcc
     2221 ccgccggcat gtaccctcag ttcaccgttc tgatttatgg tggtgttacc gacagcacca
     2281 cctgccttaa caacctgcca gcccccctcg ttaattaccg tgttcagcgc aagtccgtcc
     2341 ttgtgcacat actggtaacc gccattcagt gtggtattca gggcccttcc gtgtaccgac
     2401 tggtcaccac ctgcgtaaat gagagtgtca cgggctatcc cggaaaataa gataatctgc
     2461 cgtccatttt tgttgatggt ggtggattct gctgttccct ggaccttctg acccgtgtac
     2521 gagttgtcag aatccgggcc gccttctgcg ccggtgttga tgatggttcc tgttgccagc
     2581 ccgccacttt taacgctctg gtaaccgtcg cggttgataa ttgtaccggt ggcaaccccg
     2641 ccctcatgaa cccactgctc gcctctgtta ttcagcgtgg tgttcaccgc cagcccgttc
     2701 aggctctgtc ccccgccgtc acgaataacc gtatcactgg ctgttccccc ctccagcacg
     2761 acctgacgac catttgtggt gacagtggtg tttccggcta tcccgccatt ctgtatccat
     2821 tgcccaccgg tgttttcttc actgtccggc cccagttcca gcccggtact gatggtcatt
     2881 ccgttggccg taccgaagac aatctggttg tcatgatttg tcagtgttcc gtcgttcacg
     2941 gtttctcccg cctgtacaac cgtgtcagca gccagtgccg ggactgatgt gacagcagca
     3001 agagacagcg caaccgccac accggcgcgt tttccccgtg agcgggccag ttcggaggcc
     3061 accaccaggg tgcccgtaat gtgattccat accagcctgt agctggtgtt cagatgtcgt
     3121 ttcatcagct tttccttaca gagggtgaat aaaaaagccg gtacccacaa atgtggatac
     3181 cggcaggtac aaaacagcat ggtcagataa agtggttttt cccggacata aatgcttccc
     3241 ctctctcatc cgccagagaa gtatcagtcc gttgtgtgca attccgttct gcatgatgaa
     3301 gagattcagc tgccttttcg cagaccatta accactataa acgatcgata gtaacgatcg
     3361 atacctgttt tatcgatcgt tttattctat tgacagcaga gcggtcacgc aagagaaaca
     3421 ggagggggat gaggggtttg tgtcatgttt tcagttggtt aacgcaatgt aatcaggcat
     3481 ttcccctggc agagtgttca gaagaaaatc cagttccata cagcgcgggc aacagagacc
     3541 accgagtcac ggacggcacg cagaaccgtg cgtgcaacag aggcaataca gacgctctcc
     3601 gccgtgtcaa atatccggtc caccgcaccg gtaaactgtt cacgggcctg agaccggaca
     3661 gattccgtgc gcagctcgtc ctgcagtcgg gtcatcaggg gactggcggc atggtcggga
     3721 agcgctgtca tgagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg
     3781 gccacaaccg gatgtacggg ccggaacaga cggaataccg cctccgtttt ttcgcgaata
     3841 ttctgtgcct gtgcaggaga aggctgaata ccggccatat cccattcatg gcagggctcc
     3901 gttttgtcgg cctgcgtcac cacaaacagt acccgctgat gtccccggtg caggatgtgt
     3961 cgccagaaat actcatccac agacagggca cggtcatcgg ctttaatcag ccacagtacc
     4021 aggtccagtt caggcagaat gtcacggtac agggcctcat actctgcatc cctgtcccgg
     4081 ctctcgccca ccccgggcag gtcagtgata accatgctgt gaccgtggcc actcagacgg
     4141 aagcgccgta cttcccgggt gccggtgtga acatcactga ccggggtgac ctccccctga
     4201 aacagtgcat tacagagtga ggatttaccg gccccgcttt tacccataat gccaatcact
     4261 ggttcgtgac gggtgagttt gcgcagatgt tccaggatgt gacgggaaag cgagtaaggc
     4321 agggaggaga gcggtttttc aattgcctca atggcatcag acggattcat acatgtattc
     4381 ccggtgaaaa aagacaaaac cccgcgcacc ggaaaggtgg cgggggattc acggagataa
     4441 tatctgtctg aaattgtttt gaatcgatgg gattgttgcg tattaacatg aaggagatca
     4501 ggacttatcc gttactgggt gatctccttc catgaaaata cgccgttatt gttaaacagc
     4561 aggctgattt ctccgtaaag gggaaaatat ttatcggctg catgattatc aggtgtgtct
     4621 gtgtttactg ctgtgagtat ttttcagtgt attgtggggg agatgtcagt gggagatatt
     4681 aaatttacgt ttataattat cttacttctt ctgtgtgttt tttataagag tgcacacgta
     4741 atgatatctg ttgataccat gtggttaagt gtttcccttc ctggtcatgc gtaatgatta
     4801 aatgcttttg tgacaccatt cttccggtct gaatatttat ccagttcttt tttatttgtg
     4861 cagtgatact gtcggaagag aattcagtaa tgtgaggtgt cctggtcagg ataatatctg
     4921 ctgagatatt attagttgag tgtgttttta ctgagcaaaa gctggctttt aattcctgta
     4981 ctgaaacgct gtcgctcgtc cggggttaac tgaaactgga tgatgatgta aaatgaaggc
     5041 tgtttttgtg tttgtcgaag aagtgttatt ttaatccatt ctcatcattg gctggcgatt
     5101 atacagaagt atttgaagaa aaaatggtgc attttatctg cgtcagcagg gaggaagtat
     5161 tttattgatg tatcagtatt aaatggtttt tgttatttat aaatatgtca tcctgtccat
     5221 agaatattgt actcagcatt cagtgcagga actgtagtct tctttatttt attcttcttc
     5281 aggagggggg gattcctgaa ggcgttattc ccggtgttct gtttatgatt cttcccggca
     5341 ggaaaccggt atctgcaata cccgggatga tgctttttgt tcgtacgtgg ttgtgtaaaa
     5401 tttgaatagt ggatgtgctc tgaagggaag gaggggacac acagattcat tttgtctttc
     5461 ttcagcagca aaacacactg tggttattat ttaaccatta aagcaacaaa aatttttacc
     5521 tcatattgat ttgactgcta tttttgctat taatagccag aattgcagta cttttatatc
     5581 tgtgtgtagt gaggttacta tgtcattcgg tcagccttgt gatgagtttc ccctgagttc
     5641 gctgcctcca ctcattcgtg atgccgttat tgaagctcag caaattacgc aggctcccct
     5701 tgggcttgtg gcagcgtctg ccctgggcgc tgtttcactt gtctgccaga atctgattga
     5761 tgtctgcaga ctgaatacac tgcgggggcc ggtctctctt tttttcctga cgcttgccga
     5821 gtccggagaa cgaaaaaccg cagtggacaa attattaatg aagcccctgt accagcagga
     5881 gatgcagtta tattccagat acaaaagtga actggctgtc tggaaaaaca aggaagagct
     5941 tttaaaggct cagaaaaaag cattgttgtc aaaactgaat aaagaactcc gcaaaggcgc
     6001 ggatgagtca gaaaccctca gacaactgga agtacttcag aaaaacagtg cggaagaacc
     6061 tgtgaggtat aaattcattt ttaatgatgc cacgactgcg gcaattaaga atcagctttg
     6121 tggtaaatgg cgttcagtgg gtattatgtc agatgaagcc ggtattatat ttgatggata
     6181 taccctgtct gagttgcctt ttattaacaa aatgtgggat ggctctgtat tgtctgtgga
     6241 cagaaagaat gaacccgaac agatgattga aaatgccaga atgacgttat ccctcatggt
     6301 gcaacccggt ctttttgacc gatatatgga gcgaaaaggt tctgttgcca gggacagtgg
     6361 ttttcttgcc cgttgtctta tcagtaaacc tgcaaccacg caggggaaac gctttattaa
     6421 tggagctgtg attccgggag gatcattaac ggcatttcat gaacgattaa tggaactggc
     6481 cagaggcagt attgaaaaat ccagtgagga tgaacggtat tgtctgcatt tttcgcctga
     6541 agcgcaaaaa atatttattg agcactataa tgttctggag caggatttat ccccttccgg
     6601 acccttaagc ccgtttcggg gacatgtttc aaaaaaaaca gaaaacattg caagaattgc
     6661 agcgttattt cagtatttca gctatggtga agggaaaatc tctgccgata tcatgacgtc
     6721 tgctgtcgta ataagttcgt ggtacacaga tgaatataaa aaattatttg cgttacctaa
     6781 tgaatctgaa ctgcagcaga aggacgccga ggagctgttc gactggctca ttgaggagtg
     6841 ccgcggagaa tgcccgcccc gtgtcaggaa aaattatatt ctccagtgtg gcccgggccg
     6901 gttcagaaac aggaaaaaac tgaacgcatt gctgaacatt cttgaatctc agttcaggct
     6961 ttctgtcgtg ccggaaggaa aaaccatgta tgttctgctt ccccagatag ccagtttaaa
     7021 gttgtctgat gtctctggta tttttacctc gggttatcac tacaataagc tgagagcgaa
     7081 gtaaccgatg agcagttttt ctccattatg cttcagactt agatgattga ttattatggg
     7141 gatttcatca ccgatattca gatgtatgaa tatcggtgat gaactaaagc tcaggaactg
     7201 cgtggtggtg atgaatcaac aggcatataa ctgatacccg ccggtcagaa gttccagcct
     7261 gcgtctttcc tgtctgcgtg cccgcagtcc ggcgttcagg tgcagcgtgt gacagaacgc
     7321 acggagagcg aaacggcagg cagacaggcc gttcagtcag ccctggcacc ctgcccggat
     7381 accagcttcc ctgaaacaac tggcatcagg tgcgcttatc gtcaaccgtt gtgcggtatg
     7441 ccaggagcac gtactgatcc atcagatccg tcagtgctgc gctctgcttc tgacggttga
     7501 tgatccattc ctccagctct ttttctgatt tccgtttcag attctccgtc cagtcgtcaa
     7561 ggcagtttcc ggtcattaaa tcaagccagc aaagccagac cagtttgagg cgcaactccg
     7621 tgccaccaaa ctgacggaag aggctgatca gtttatttgc cagtgaacgc tgcctcagac
     7681 gttcacattc agacaggaga atgccttcca gcatcggatg atgaaccagc agatgttcac
     7741 tggtgcataa attcactatc agatcatcat ccaggggcgg cgtggtccgt ttgtccggac
     7801 cataaatcag ccggtgcaga ctctggggta cgatggcaat ctccggtttt tcaccattga
     7861 atgacaccca cccgggcgtc agattatgga agtagtagat attcggatga cacacgcccc
     7921 aggatacggc gtgccgggtg aagccggtca gccatttctg gtagctttcg tggagcagct
     7981 cgtaacgggt ctgatactgt ggtttcattt tcatattcct cacgggggat aacaggcagc
     8041 agaacgccgc cggcaggccg gagactgcgt ctgctgcaga tgaacggtta agaattacgg
     8101 acgggactgt gcaatacgcg cctgcagcca ggcttccact tcgcttttca gccatcggga
     8161 gctgcggccc attttgatgg gcgcaggaaa gtcaccatcc ttgatgagtt tgtaaaacca
     8221 cttatcggtc aggccggtca gttgagtgat aaatgccatg tcgaccatct ggtcatccat
     8281 cagtgaaact ggggtgatca tgagaatgtt cctccggatg ttgttaaacc agccggtgtg
     8341 gtcatgaaaa caacgcacca ccttcaccgg cataacaacc ggggagaaac gtaaaaaata
     8401 atcgggaatg tgaccgccag gagataaacg gggaggttac ggtggctgag ccgtaccggt
     8461 atgccgggag gttgttacga aacgatgctt ccgcacgcgc gcgcgataaa gaagggatga
     8521 ttttcgtgag gaagagggga aaactggcgt tctgataatt gcagagttat ttacggggcg
     8581 gttacgatgg ggcagaccgt gtccggtggg caggtgacag gtatcaccga tgggataaga
     8641 ctgtctgatt ggtattataa atatataaga gagatcaaac acatgagatc aaatacatga
     8701 gttcatatat gtgaaataaa cacggtgagt gtgatcagtg ggcatgatga gaacgcagta
     8761 gcaacagact gcgctatccc tgatagctgg cgtataaccc gcgtatataa aacgtatctg
     8821 caatgtacgt gaacagagtg cattaagcat ccgtctgagc ggtaaacata tccgacctgg
     8881 tgagtgacag tctgagagac cggttaagtg gtggctcggg agtaatgagt ttggggtggg
     8941 taccagaatg ccagtctggc ggtatgctga cagtatcacg ttaattgcag cataaagtga
     9001 tgagaggcta atggaagtta aaaccaaagc agttccgtcg ttgttcatcc cgggagacgc
     9061 tggaaaaagt gatttcccac acgcgttata aacttacctc tgcggaactg gaagccttca
     9121 actctgcggt cgatcaccga ctggcagaac tgacaatgaa caagctttac gatcgtgttc
     9181 ccgcttcagt ctggaaatat gtcacctgaa cgttcggtaa tcacagaggt attgcgccag
     9241 gtgttatctg ctctgtgtga ttctttttgg gttttctcag gttgttaaaa gctgaatatg
     9301 ttcctgttgt cgggaaccag gataataccg ggaacgttaa gcggtatagc gacaacaaag
     9361 aattcgaaat caaaagggaa tggtgccgga ggcgtgggcg gtaatgggcc gattttcggg
     9421 cattaccgac ctctgctgag gttttctcgt atttatcggg gagctggtgc gttactgtca
     9481 ctgtctggca cagcaaacgc agaggtggcg gggcgctctg ctccgctgac tccggctaat
     9541 ctctcccctt ttacctactg caacagtaca aatacactga cgcctgaaac gacagccaga
     9601 atcagcacag gcagtaacgt cagtagacaa agcagacgat cggtattccg gtaataatcg
     9661 aagggatctg ctgacacgta gcccgggcaa cattccgtat tatgagggat ggcgttatca
     9721 caatccctgc acaccgtttt gcgtatcacc atcacgtcct cctcacaatc ttatccgtaa
     9781 ccggcttttt gcattaaaaa aacaacgctg aaaattcagc cgtcagtccg gagacgaccg
     9841 ttcgggttat ctcagagtgc ctgagatatt gcctggttgg agatcacttc agggactctg
     9901 cgcatttttt acggcgtggt taatccgtat gaacgaaacc atacggagat ttaaccatga
     9961 cctacaaata caaccccttc tggcagcaac gtattcgtga gacggtgcgg cacgcactgg
    10021 atgttcatcc ccgtctgacg gcattgcggg ttgatctgcg tctcccggat gtaccggcag
    10081 caacggacgc ggctgtgata tcccgcttca tcaatgccct gaaagcccga atcgacgctt
    10141 accagaaacg taagcatcgg gaaggtaaac gcgtgcattc ctcgaccctg cattacgcct
    10201 gggcccggga gttcggggag cttaaaggca aaaaacacta tcacctgctg ctgctggtca
    10261 accgggatac ctggtgtcgt gccggcgatt accgcgctcc ggggtcactg gccgggatta
    10321 ttaaacaggc ctggtgcagt gccctgggag tggatgccgg acgttatgac acgctggcac
    10381 attttcctga caagccagcg gtgtggctgg aacgcgatga tgaaaccggc tttcagcagg
    10441 tgctggaacg ttctggctat ctggcgaagg agcataccaa agctcgcggc accggtgagc
    10501 gcaattttgg ctgtagccgg agttgaacac gacaggcgca ccaatacccg gcaaaccaaa
    10561 agtatgggtt gttgtctggc ggcaacgatg tatccactcg cccactgact gttttttacg
    10621 tcagtgggcc attgtgtgac aaccacagtg ctgtaacagc aaccagcctg ctttacccaa
    10681 actccagtcc agattttaac tgaaccgcca ttcacacctg accacgctgc ccgtacctgg
    10741 acggctgttg cctgcgtgtc gtctggcggc tgaggaatat atactatgta cgcaaaatcc
    10801 tttctcgctc ttgatggcaa cggacgtctg acgggggccc gtaccgcaca gactgcgcct
    10861 tacgaccgct acacctgcca cctttgcggc agtgcactca gataccaccc gcaatacgac
    10921 actgaacggc cctggtttga acacgctgac gaagggctga cggagcacgc gcagcaatgt
    10981 ccttatgtca ggccggaacg cagagaaata cagttgatta aacgtctgca gcaattcgtc
    11041 ccggatgcct tacccgtggt gcgtaaagcc agctggcact gcagacaatg tcaccacgat
    11101 tattatgggg agcggtactg cacacactgc cagaccggac gcttcagtga ggaggggggt
    11161 agcagaatga acggtataaa aacggggctg gggatcacgc cgggtgaaca cattatcagt
    11221 gccgactctg cgctcagcag aaatatccgg cattgtttct gcctttcctg ccatggccgg
    11281 ttgattctgc agacagatgc tcagggggca tggtttgagc atgatttaca tgccctgagt
    11341 gagcaacaga aggcggactg tgtggtgctg aatccggaaa agtcccaccc ttacattgag
    11401 gatatggcga tgtttttatc gccattatcg gtcgtgctgg agtggcattg tgtgatgtgt
    11461 gagcagtttt ttcatgggaa gaagttctgt gaggcctgtg gtacggggat ttattgcaga
    11521 gcggtatgta cgaagtctgt ttacagctac cagccagact tgtttgagga ctgcggaggt
    11581 gcgagtactt aatccgaaat ccccgctctg cgcagggcgt tcagaggtat tgccaacctg
    11641 tattttatcc ggtgtttcgc acccaccccg ttcgtgacac aacggcgcga caccggatat
    11701 ccgcagtcct gcttacctga ataaaacccg gggtattgat tggtagtgtg gtgcggaatc
    11761 gtaccgtgat ctcgcatccg ccgaattcgc actgtggtgt caatcaacca tacgcgagag
    11821 tcctctccgt atttatcagt gcctcaggat gatttctgca tagttaatcc agggggctgc
    11881 attgttcatt tccctgctaa tggacgtgac atccaggata ttaatgctcc tgatttccct
    11941 gagcagtatc agcgcttatt ggcacgtctg gattatctga cgaagcccga tgctaaagca
    12001 tcaggccagc gtaactctgg ctacagcggt ttttgacctg ttgtaactgt aaggtgattt
    12061 attatgtata cagaatcaaa tgttaagctt tcctgtaacg ccctctggca aaagtgtgtc
    12121 cttgcaactc tgtatcgggc tgcagccaca catcctgaaa ttaaattatt acaatttaaa
    12181 ttaataccag ggcagggtat taataaggcc attccctctc tgatggctga ttttactggt
    12241 gaactgcata gactcatttc tgaatatttg cagtgtaagc gacgtaaaga tcaatctgtt
    12301 gagcccaccc ctttatatta tctctggagt agggaagctg tggctaacga taccactcct
    12361 tatcttatgg tgctgctact taataacgat gtctggtgta agagtcgtga ggtggtggtt
    12421 ctaagaaatc tgattgagca ggcgtgggga aaagtattga gtgaagataa ctccattaca
    12481 catctggttg tgtatcctga agtgttgtcg ttggtatttg atgcagctaa cacatacaac
    12541 gctgagcctg gtgtactgga acagacggcc cgggcttaca ttgaatccta tggtgccatt
    12601 gaacgtcatc ttgggtgtag tgacttttaa cgtcaggtga cgcattgtca cttcagacct
    12661 gctacctgta cgccggaata taatggtcag agaggcatat ttcgacgccc ctaatctggg
    12721 gcgtttgatt caggatgtct ggaacaatcc gggctttgtg tcaccgggcg catcaaagct
    12781 gtaccacccg ggatgtggcg ctggatcgtc atttaccgga ctgcgaagac atacttaaag
    12841 aggtgatatg ggcgttcagt gattttgtcc gcgataatcg tggtgtgtat gatccggaag
    12901 cccgttaccc cgccggtaat ccctggtatc ccgtaacagg ccagttctga aagcacagct
    12961 cagcagattc cccgcattcg cacaatcttc ataaatctca caggacattc tgaccatgtt
    13021 gactacaaca agccacgaca gcgtatttct gcgtgccgat aattccctga tcgacatgaa
    13081 ctatatcacc agtttcatcg gtatgaccga caaatggttt tacaagttga tcagtgaagg
    13141 ccatttccct aaacccatca aactggggcg cagcagccgc tggtacaaaa gtgaagtgga
    13201 gcagtggaag tgaagtagag cagtggatgt agcaacgaat cgaggaatcc tgggggcagc
    13261 agcatgaaac gtgttgtaat gccggtacgc tggcaatgtg caaaatgcca gcgctggtat
    13321 tgtggaaccc ggccctgccc ttggtgctgg cgatattccc gttcatcttc ctgctgatac
    13381 cttccgttca gcagcgggtt gttatccctt cctgactgat tcgtcattcc attcttattg
    13441 attagaactg gcattacacc ggtgccggcg tgctttcctg cgtgtctgcg ccggttcagt
    13501 aaattagaaa agaaacattt gtcagcgcca gtgatataag gcgtaacggc taacaatctt
    13561 ggcgttaata cattccgagc aaataaccga agcgttactt ttagtgacgg ggttatccaa
    13621 tacttttcta gtcagtgttt agtatttatg caagcgatat aagtagtcac cgcttagact
    13681 attgacattc atgatgcctg ttaattcaaa tttcaggtat gaacagtcat ttattgttgt
    13741 gatactgatt ccgctagttt agcataactg tcctgcaaat cattgttcaa aaaagaaagc
    13801 tcctacaaa
//
LOCUS       DAZKHK010000127        13638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKHK010000127 DAZKHK010000000
VERSION     DAZKHK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.127"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1544
                     /locus_tag="ACHOS5_004031"
     CDS             <1..1544
                     /locus_tag="ACHOS5_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFP9778764.1"
                     /translation="AEGTTQAAVADGTITIRDRDNQKQNLANLSRDPAHANDSISPIF
                     DKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAQDKYGPVPADATEEQRQAYL
                     AKLRDTPEYKKEQEKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPELAHLLK
                     STEKDPAVNAIAHAILGGAVAAMQGNNVAAGAAGAATGELAARAIAGMLYPGVKQSDL
                     SEEQKQTISTLATVSAGLTGGLTGNSTASAAVGAQSGKNAVENNYLSNKQIDAWSAEM
                     KSCQAKGGDCGGIIKKYEELSTAQQKQLIRDCAASPATCQQKYGDVLTNSLAVKLAID
                     RALGEDIPIKMVYDLTATFAQQMQAEGVVATNKVSEALQKEYGLDEVQAGIVASAAAS
                     AFGGIGKAKGNSQPKIEQILKPEKNWETARNKALDLVGNLGADSKPVIGRLEVSAGNG
                     KVIGRQSSDGKVGWRVDYDPEKGTHINIWDYSQGKGPGKAVKQVIPFEGNEKSFETIL
                     KQLNR"
     gene            1550..1924
                     /locus_tag="ACHOS5_004032"
     CDS             1550..1924
                     /locus_tag="ACHOS5_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778765.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEALGILNKYPLAKGSVTWSE
                     IRHSDYESFDELLSANSVKNDDMFVFADDASIPVFRSNLRLIAENIYDVTALSPKLFI
                     FNDEVIIQPLFPTDMFRLGIKK"
     gene            2180..3352
                     /gene="istA"
                     /locus_tag="ACHOS5_004033"
     CDS             2180..3352
                     /gene="istA"
                     /locus_tag="ACHOS5_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFP9778766.1"
                     /translation="MLGREDFYMIKQMRQQGAYIVDIAAQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWSEVGVEVAGQRCKVNFAVNTLGFSRRFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHQPLSAYEELL"
     gene            3352..4149
                     /gene="istB"
                     /locus_tag="ACHOS5_004034"
     CDS             3352..4149
                     /gene="istB"
                     /locus_tag="ACHOS5_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFP9778767.1"
                     /translation="MHELEVLLSCLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLVKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
     gene            complement(4210..4893)
                     /locus_tag="ACHOS5_004035"
                     /pseudo
     CDS             complement(4210..4893)
                     /locus_tag="ACHOS5_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            complement(4913..5260)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004036"
     CDS             complement(4913..5260)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9778768.1"
                     /translation="MISIPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            complement(5260..5909)
                     /gene="tnpA"
                     /locus_tag="ACHOS5_004037"
                     /pseudo
     CDS             complement(5260..5909)
                     /gene="tnpA"
                     /locus_tag="ACHOS5_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            complement(6201..7565)
                     /gene="pgtP"
                     /locus_tag="ACHOS5_004038"
     CDS             complement(6201..7565)
                     /gene="pgtP"
                     /locus_tag="ACHOS5_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter PgtP"
                     /protein_id="HFP9778769.1"
                     /translation="MLSVFKTGQAADSVPAEKIQVTYRRYRMQALLSVFLGYLAYYIV
                     RNNFTLSTPYLKEQLDLSATQIGVLSSCMLIAYGISKGVMSSLADKASPKVFMACGLV
                     LCAIVNVGLGFSTAFWIFAALVILNGLFQGMGVGPSFITIANWFPRRERGRVGAFWNI
                     SHNVGGGIVAPIVGAAFALLGSEHWQSASYIVPACVAIVFAVIVLILGKGSPRQEGLP
                     SLEEMMPEEKVVLNTRQTVKAPENMSAFQIFCTYVLRNKNAWYVSLVDVFVYMVRFGM
                     ISWLPIYLLTVKHFSKEQMSVAFLFFEWAAIPSTLLAGWLSDKLFKGRRMPLAMICMA
                     LIFICLIGYWKSESLFMVTIFAAIVGCLIYVPQFLASVQTMEIVPSFAVGSAVGLRGF
                     MSYIFGASLGTSLFGIMVDHIGWHGGFYLLGCGIICCIIFCWLSHRGAIELERHRAAY
                     IKEH"
     gene            7964..9181
                     /gene="pgtC"
                     /locus_tag="ACHOS5_004039"
     CDS             7964..9181
                     /gene="pgtC"
                     /locus_tag="ACHOS5_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transport regulator PgtC"
                     /protein_id="HFP9778770.1"
                     /translation="MILLLLLMDAGAVRAQRNELVMATTFSPGATAWIIQRWQTEPES
                     VMVRTLNRTSASLEQLLDTANVENVDLILTSSPMLLQHLQEHQKLAPFDDAPAESQNL
                     VPESIRATSVAVAISGFGLLINRPALSVKHLPAPADWDDLALPIYQDALLMSSPSRSD
                     TNHLMVESLLQQKGWVKGWETLLTSAGNLVTISSRSFGVADKIKSGLGVAGPVIDNYA
                     NLLLNDPHLSFTYFPRSAVSPTYVAILRKSPHADAARRFIHYLLSPKGQRILADANTG
                     KYPVTPLAADNPRATQQQLLMNQPPLNYHLILKRQRLVQRLFDTAISFRLAQLKDAWR
                     ALHSTEARLKKPLPEIRALLAQVPVAAASSEDPFWLAQFDNKSFAEQQMMEWQLWFLN
                     NQRLAIKKLEELK"
     gene            9178..11187
                     /gene="pgtB"
                     /locus_tag="ACHOS5_004040"
     CDS             9178..11187
                     /gene="pgtB"
                     /locus_tag="ACHOS5_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PgtB"
                     /protein_id="HFP9778771.1"
                     /translation="MKNITLWQRLRQVSISTSLRCAFLMGALLTLIVSSVSLYSWHEQ
                     SSQIRYSLDEYFPRIHSAFLIEGNLNLVVDQLNEFLQAPNTTVRLQLRTQIIQHLDTI
                     ERLSRGLSFRERQQLTVILQDSRSLLSELDRALYNMFLLREKVSELSARIDWLHDDFT
                     TELNSLVQDFTWQQGTLLDQIASRLGDTAQYLKRFREVQNEQQQVYTLARIENQIVDD
                     LRDRLNELKSGRDDDIQVETHIRYFENLKKTADENIRMLDDWPGTITLRQTIDELLDM
                     GIVKNKMPATMREYVAAQKALVDASRTREATLGRFRTLLEAQLGSTHQQMQMFNQRME
                     QIVRVSGGLILVATALALLLAWVFNHYFIRSRLVKRFTLLNQAVVQIGLGGTETAIPV
                     YGNDELGRIAGLLRHTLGQLNVQKQQLEQEITDRKVIEADLRATQDELIQTAKLAVVG
                     QTMTTLAHEINQPLNALSMYLFTARRAIEQTQTEQASMMLGKAEGVISRIDAIIRSLR
                     QFTRRAELETSLHAVDLAQMFSAAWELLAMRHRPLQATLVLPQGTATVSGDEVRTQQV
                     LVNVLANALDVCGQGAVITVNWQMQGKTLNVFIGDNGPGWPEALLPSLLKPFTTSKEV
                     GLGIGLSICVSLMEQMKGELRLASTMTRNACVVLQFRLTDVEDAK"
     gene            11177..12424
                     /gene="pgtA"
                     /locus_tag="ACHOS5_004041"
     CDS             11177..12424
                     /gene="pgtA"
                     /locus_tag="ACHOS5_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PgtA"
                     /protein_id="HFP9778772.1"
                     /translation="MLSSEYSILLIDDDDDVLDAYTQLLEQSGYRVFACNNPFEAQAW
                     IQPDWPGIVLSDVCMPGCSGIDLMMLFHQDDQQIPILLITGHGDVPMAVDAVKKGAWD
                     FLQKPVDPGKLLSLVEEALRQRQSIIARRQYCQQTLQVELIGRSEWINQYRRRLQQLS
                     ETDIAVWLYGAPGTGRMTGARYLHQFGRNAQGEFVYRELTPDNAPQLNDFIALAQGGT
                     LVLSHPEHLTREQQYYLVQLQSQEHRPFRLIGIGDTSLVELAASNHIIAELYYCFAMT
                     QIACLPLAQRPDDIEPLFRHYLCKACQRLNHPVPEVGKEMLKEMMRRMWPNNVRELAN
                     AAELFTVGVLPLAETANPLVHVGTPTPLDRRVEDAERQIITEALNIHQGRINEVAEYL
                     QIPRKKLYLRMKKYGLSKEHYKN"
     gene            12750..13605
                     /gene="istA"
                     /locus_tag="ACHOS5_004042"
                     /pseudo
     CDS             12750..13605
                     /gene="istA"
                     /locus_tag="ACHOS5_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         3711 a         3206 c         3535 g         3186 t
ORIGIN      
        1 atgcggaagg aacgactcag gccgcagtgg cagatggcac aatcaccatc cgggacaggg
       61 acaatcagaa gcagaatctg gcgaacctga gccgtgaccc tgcgcacgct aatgacagta
      121 tcagcccgat atttgacaag gagaaagagc agaggcgtct gcagacagtg gggcttatca
      181 gtgacattgg cagtcaggtg gcagatatcg cgcggacgca gggggaactg aatgcattga
      241 aggctgcgca ggataaatat gggcctgttc cggcggatgc gacggaagaa cagcggcagg
      301 catatctggc aaaactgcgt gatacgccgg aatacaaaaa ggaacaggaa aagtatggca
      361 ctggcagtga gattcagcga ggtatacagg ccgcgacagc cgcgcttcag ggactggcgg
      421 gtggtaattt agcgggtgct ctggccggag cttctgcacc ggaactggcg catctgctga
      481 aatcaacaga aaaagacccg gcagtaaacg ccattgctca cgccattctg ggcggtgcag
      541 tagcggcaat gcagggtaat aatgttgctg ccggtgcagc aggcgcagca acgggagagc
      601 tggcagccag agcgattgcc ggaatgctgt atcctggcgt gaagcagtca gacctcagtg
      661 aagagcagaa gcagacgata agtacgctgg caaccgtttc agcagggctg accggcggtc
      721 taaccggaaa cagcaccgca tcagcagcag tgggagcaca gagcgggaag aatgctgttg
      781 agaataacta cttgagcaat aagcagatag atgcctggtc cgctgaaatg aagtcctgtc
      841 aggcgaaagg cggtgactgc ggcggtatta tcaaaaagta cgaagagttg agtacggcac
      901 agcagaagca actgatccgt gactgtgctg ccagcccggc aacctgccag cagaaatacg
      961 gtgatgtact gacgaacagt ctggcggtga agctggcaat agatcgtgct ctgggtgaag
     1021 atatcccgat caagatggtc tacgatctga cggcaacttt tgcgcagcaa atgcaggccg
     1081 aaggggttgt cgcaaccaac aaagtctcgg aagcactcca gaaagaatac ggtctggatg
     1141 aagttcaggc gggaatagtc gcgagcgctg cggcgtcagc atttggggga attggtaagg
     1201 ctaaagggaa ttcccagcct aaaattgaac aaatcctcaa gccagagaaa aactgggaaa
     1261 ctgctcgaaa caaggcattg gatcttgtag gtaatttagg agcggactct aagccagtta
     1321 tcggacgttt ggaagtcagc gctggtaatg ggaaagtcat agggcggcaa tccagcgacg
     1381 gaaaagttgg ttggcgtgtt gattatgatc ctgaaaaagg cacacacatc aatatatggg
     1441 attactcaca gggtaaaggc cctggaaaag cggtgaagca agtcatacct tttgaaggca
     1501 atgaaaagtc ttttgaaaca atattgaaac agctaaacag gtagagcaaa tgactttatt
     1561 tgatgagtgt cgggaagcac tgagtgctga ttttaatata gtagaggggc tggctcagca
     1621 ggaggctctg ggcattttaa ataagtatcc tctcgcgaaa ggaagtgtta cttggtcaga
     1681 gattaggcat tctgattatg agagttttga tgagttattg agtgcaaact ctgtaaaaaa
     1741 cgatgacatg tttgtctttg cagatgatgc aagtattcca gtttttaggt caaatctaag
     1801 attaatcgct gaaaatatct atgacgtaac agctttatcg cccaaattat tcatttttaa
     1861 tgatgaagtg ataatacaac ctttattccc aactgatatg ttccgtttag gaatcaagaa
     1921 atagtgggaa agctcgcaaa tcccggccat tatgccggga tttttttgcg catcatctgc
     1981 cacagcttta cacggttttc ctctcaacag cgggacaggg taacaacgca aaaaagcctg
     2041 tggagaaaat gaaacaagga aaaccatttg taactaactg ttagcgccag tgatataaga
     2101 cggtcattca ccatttggat tgtccgctcc acccaacatg ttgtttcctt aaggttctca
     2161 caccaaaaag gacatcaaca tgctgggcag agaggacttt tacatgataa agcaaatgcg
     2221 ccagcagggc gcgtacattg tcgatattgc ggctcagatt ggttgctctg aacgaacggt
     2281 cagacgctat ctcaaatacc ctgaaccgcc agccagaaaa acccgccaca aaatggttaa
     2341 gctgaaaccg tttatggatt acatcgacat gcgcctggca gagaatgtct ggaatagcga
     2401 ggttatcttc gcggagatta aggcaatggg ctatacgggc ggacgttcca tgctgcgtta
     2461 ctacatccag cccaaacgta aaatgcgtcc gtcaaaaaga acagttcgct tcgaaactca
     2521 gcctggatac cagctccagc acgactggag cgaagttggg gtggaggttg cagggcaacg
     2581 gtgcaaagtt aactttgcgg ttaatacgct ggggttctcc cgccgcttcc atgtcttcgc
     2641 cgcaccaaaa caggatgctg agcataccta cgaatcactg gttcgcgcct tccgctactt
     2701 cggtggttgt gtgaaaacgg tgctggttga taaccagaag gctgcggtgc tgaagaataa
     2761 caacgggaaa gtcgtgttca actccggatt cctgttgctg gccgaccact ataacttcct
     2821 gccacgggca tgccgtccac gcagggccag aacaaaaggt aaggttgagc ggatggtgaa
     2881 atacctcaag gagaacttct tcgtccggta ccgcaggttc gacagcttca ctcatgtcaa
     2941 tcaacaactg gagcaatgga tagccgatgt ggctgacaaa cgggaacttc gccagttcaa
     3001 agaaacgccg gaacagcgct tcgcgctgga gcaggaacat ctgcagccgt taccggatac
     3061 ggacttcgat accagttact tcgacatccg ccatgtgtcc tgggacagct atatcgaggt
     3121 tggcggtaat cgttacagcg ttcccgaagc gctgtgtggt cagccggtat cgatacgaat
     3181 atcgctggat gacgagttgc ggatctacag taatgagaaa ctggtggcct cacatcgcct
     3241 ctgttcggca tcgtctggct ggcagacagt gccggagcat catgccccgc tctggcagca
     3301 ggtcagtcag gtggaacatc aaccactgag tgcctatgag gagctgttgt gatgcatgag
     3361 ctggaagtcc tgctgagctg cctgaaaatg gagcatctga gttatcacgt tgaaagcctg
     3421 ctggaacagg cagctaaaaa agagctgaac taccgggagt tcctgtgcat ggcgctacag
     3481 caggaatgga acggcaggca tcagcgcggt atggagtccc ggctgaagca ggctcgtctg
     3541 ccgtgggtca aaacgctgga gcagttcgac tttaccttcc agccgggcat cgaccgtaag
     3601 gttgtccggg aactggctgg tctggcgttc gtggagcgca gcgaaaacgt gatcctgctg
     3661 ggacctcctg gtgtcggaaa aacgcatctg gccatagctc ttggcgtaaa agcggtggat
     3721 gcgggacatc gggtactgtt tatgccactg gacagactga tcgcgacact ggtgaaagcg
     3781 aaacaggaaa accggctgga gcgtcagtta cagcaactga gttatgcccg ggtgttgatc
     3841 ctggatgaaa taggttatct gccgatgaac agagaggaag ccagcctgtt cttccggcta
     3901 ctgaaccgtc gatatgaaaa agcgagcatc atactgacgt caaacaaagg gttcgcagac
     3961 tggggagaaa tgttcggaga tcacgtgctg gcaacagcga tcctggaccg gctgctacat
     4021 cactcaacta cgctgaatat caaaggagag agttaccggt taaaagagaa acgtaaagca
     4081 ggagtgctga ccaaaaacac aacgccaatc agtgatgatg aaatggtgga aagcggacag
     4141 catcagtaac gaaagtatct tagcgggcat gaaaatggca aataacggtt aaaaatcgtg
     4201 gcgttgacac cccagcgtgg cgcggctcac ctccacaccc tgacgacggt atatttctga
     4261 ctggcggtat aacggcagat ggtctgcata cttcccggtg acgacatggg ccagaagccc
     4321 cgctccggca tagctgcgtg caatgggttt tgaagacacc ggtgcctgca cgatatggtc
     4381 gcaccggcaa caggccagtt tcggacgttg tgtttcgata accttaaagg cgctgctgat
     4441 aagctccagt tgctctgaca catcacatcc cagagaactg agttcgccac cacaggccgg
     4501 gcagcattcc tcttccggcc ggataacccg gctttcacgt ggaagtgagg ccggtaacgg
     4561 tttacgggct gaagactggc gcagggcgga tggcagtacc ggatcatact gctcacccag
     4621 cgtttctgcc atttcttcct gaagtgcgct gattcgctcc tgtgcttcct gtatctgtcg
     4681 ttcggttttt gcgcgaagtt tttctgagct tttaccgaac tgcatgcgct gtagttttgc
     4741 aaccagcgcc ttcagccggt tgatttcaga agcataagcc gccacccgct gtgagagcag
     4801 gcagttgtat tcggccatct ggcggatggt gtcctgttgc gtctgcaaca gtgcccgcag
     4861 gcgggcattc tcatgagcaa gtgaggtgtc catatcctca ctttacaacg ggttatatgc
     4921 ggattccagc gcgttccgtt cgtttcgggt gcttccagtt gatgccctca agaagcatgg
     4981 acaactgagc cggagtaagg tgaacctttc catcgcgggt gaccggccag acgaagcggc
     5041 cccgctctag gcgtttggtg aagaggcaca gtccgtcgct gtcagcccac aacactttta
     5101 tctggtcacc ccggcgtccg cggaagatga acaggtgtcc ggagaacggg tcatccttca
     5161 ggacgttctg aacttttgac gctaggccgt taaagccatt tcgcatatcg gtgatacctg
     5221 caaccagcca gatacgcgaa ccggcaggga tagatatcat cagtggctgc tcccttttat
     5281 ttcacggata agtgtctgta ataacgccgg cgtcagttta cctttcagcc tgagagttcc
     5341 ggccggcaga accagctcac agcacagact gtcggacggt gtatttgtct gttcaggtat
     5401 ctgcactggt gccgggattt tattatccgg ctccggagtt aacgtcacgg gaagtagcga
     5461 tgtttcatgt tcgccgggcg actgcaggcc gccgttacgc cagagatgcc ccagttaaag
     5521 agcaggttat cgttgattcc gttctcacga gctatttgtg cgacacaggc accgggtaaa
     5581 agagtttgct gaacaagtct ccatttaaac tccggtgaaa attggttccg gcgggttttc
     5641 tctgttttca cagtctgagt tactgtcggg gctgaagggt atcgccccgg gaacagagcc
     5701 cgggccagtt gcacgcagga atattccccg gaaagaggcc agtcagtgaa tacctggtga
     5761 aatcgcctca ctagagaaaa aatggcggtt ttgctgatac cggtctgcgc agaaatgcgc
     5821 gggtacgaca gtgactcgtc aaatctcagt tgtagcacac gcaggatcaa ttccggattg
     5881 tctgccagta ccgcttttcg tgcattcatc ttaaatgtcc ctttactgcg aaaatggaca
     5941 ttagtatcgg aaacaggaaa gggaggcgaa agacggttta aatgagacgg ttactgtttt
     6001 ttagcatttt caatgcataa caattgcgaa ttatatgcgt tatctgattg agacaaaaga
     6061 tatccatttt tctcatgaca ataccatctc catttattgt catcagtttt ataaaatttc
     6121 catattaatc tctgattgcc agatatctta taaatcataa tatgctccac tccccaggga
     6181 aacgaccctg gggaaggtaa tcagtgctct tttatatatg cggctctgtg acgttcaagt
     6241 tcaattgcac cacgatgtga taaccagcaa aaaatgatgc aacaaataat accgcagcca
     6301 agaagataaa atccgccatg ccagccaata tgatcgacca taataccaaa caggctggtg
     6361 cccagagatg caccgaagat atagctcata aaaccacgta aacctaccgc ggagccaaca
     6421 gcaaagctgg gcacgatctc catagtctga acggaagcca gaaattgtgg aacgtaaatc
     6481 agacaaccaa caatggcagc aaaaattgtc accataaaca acgattcact tttccagtag
     6541 ccaatcaggc aaatgaaaat cagcgccata caaatcatcg ccaatggcat acgacgccct
     6601 ttaaacagtt tgtctgacaa ccaaccggca agtagcgtgg aagggattgc ggcccattca
     6661 aaaaataaaa atgcgacgct catttgttct tttgaaaaat gtttcaccgt cagcagataa
     6721 ataggcaacc agctaatcat cccgaagcgc accatgtata caaatacgtc aaccagtgag
     6781 acataccagg catttttatt gcgtaataca taagtgcaga aaatctgaaa ggcgctcatg
     6841 ttttctggtg cttttaccgt ctgtcgggta ttcaggacga ctttttcttc cggcatcatc
     6901 tcttccagag agggtagacc ttcctgacgt ggggaacctt taccgagaat cagcacaatt
     6961 accgcaaaaa cgatggccac gcaggccgga acgatatagc tcgcactttg ccagtgctcg
     7021 ctgccgagta gggcaaaagc ggcaccaaca atcggtgcaa caataccacc accgacgtta
     7081 tgagagatat tccagaaagc accaacccga ccacgctccc gacgagggaa ccagttagca
     7141 atagtgatga aagaaggacc aacgcccatt ccctggaaaa gaccattcag aataaccaat
     7201 gccgcaaaaa tccagaatgc ggtgctgaat cccaggccaa cgttaacgat ggcacataac
     7261 accagcccac acgccataaa gacttttgga ctggctttat cggcaaggct actcatcact
     7321 cctttgctga taccataggc gataagcata cagctactca gtacgccaat ttgtgtggcg
     7381 ctgagatcta attgctcttt aagataaggc gttgataagg tgaaattatt acgcacgata
     7441 tagtatgcaa gataccccag aaatacgcta agtaacgcct gcatacgata gcgacgatat
     7501 gtcacctgaa ttttctccgc cggaacacta tccgccgctt gccccgtttt aaatactgat
     7561 aacatgtctt cacctgtatt ttatattgat atgaacatca ggagtattct gacaatgaga
     7621 gttatataat tgagatgaaa tatcaaactg aaaaaatgtg tcacaattga cacaaaataa
     7681 taaagtcaaa gcctgttttt gacccatttt attgatgcat agacagggca gatttatgct
     7741 ttatctggtt tgttgttagc atgtgcggat attaacaaat ctcatgctga ttaccgggta
     7801 atctctgatt aatatattaa cggtctcttt gagcagacgt gttatcagac gaagtgtctg
     7861 aaacgggtaa tttttgtctc aatgtgattt ttgcggtgta ttaatattta tgatctggat
     7921 cacaaaccga ttttttagct ggtgcgaaat cggcatcaaa accatgattc ttttactgtt
     7981 gttgatggat gccggggccg tcagggctca acgaaatgaa ctggtgatgg ccaccacatt
     8041 ctcacccgga gctaccgcgt ggataataca gcgctggcaa acagagcctg agtcggtaat
     8101 ggtccgtacg cttaaccgca ccagtgcctc actggaacag ttgcttgata cggccaacgt
     8161 agaaaacgtc gatctgatcc tgacttcatc accaatgctg ctccagcacc ttcaggagca
     8221 ccagaaactg gccccgtttg atgatgcacc cgcagaaagc caaaacctgg tgccggagtc
     8281 gatccgtgca acctccgttg cagtagcaat atcaggtttt ggtctgctca ttaatcgtcc
     8341 ggcgctttct gtaaaacacc ttcctgcccc tgctgactgg gacgatcttg ctttgccgat
     8401 ctatcaggac gctttattga tgagtagtcc gtcgcgttca gatactaacc atttaatggt
     8461 tgagtcatta ctacagcaaa aaggctgggt gaaaggatgg gaaacgttgc tgacaagtgc
     8521 aggaaacctg gtgacaatct cctcccgcag ttttggcgta gccgataaaa taaaaagtgg
     8581 actcggcgtt gccggccccg ttatcgataa ctatgctaat ttactgttaa atgaccccca
     8641 tctctctttt acctatttcc cccggtctgc ggtatccccc acctacgttg caattctcag
     8701 gaaaagccca cacgccgatg cagcccgtcg ctttattcac tacctgttaa gtccgaaagg
     8761 acagcgtatt ctggccgatg ccaatacagg aaaatatccg gtgacaccac ttgccgcaga
     8821 caatccgcgg gcaacgcaac aacagctcct gatgaatcaa cccccgctga attatcacct
     8881 tatcctgaaa cgtcagcgtc tggtgcagcg cttgtttgat acggcaatta gctttcggtt
     8941 agcacagcta aaagatgcct ggcgagcgct gcacagtacc gaggcacgtc tgaagaaacc
     9001 attaccagaa atacgtgcac tgttagcaca agtgccagtt gcagcggcca gcagtgaaga
     9061 tcccttctgg ctggcacagt tcgataacaa aagctttgcc gagcagcaaa tgatggaatg
     9121 gcaactctgg tttctgaata accagcgcct ggcgataaaa aaactggaag agctgaaatg
     9181 aaaaacataa cgctgtggca gcgtttaaga caggtcagta tcagtaccag cttacgttgc
     9241 gcatttctga tgggggcact actgaccctg attgtcagta gtgtcagtct gtattcatgg
     9301 catgaacaaa gctcacaaat tcgttactcg ctggatgagt attttccccg tattcactct
     9361 gctttcctta ttgaagggaa cctgaatctg gtggtagacc agctaaatga atttttgcag
     9421 gctcccaaca ccacggtgcg attgcaactt cgtacccaga ttattcagca tctcgacacc
     9481 atagaacggc ttagtcgggg actgtcattc cgggaacgcc aacaactgac ggtcattttg
     9541 caggacagtc gatcactgtt atccgagttg gatcgtgcgc tttacaacat gtttttacta
     9601 cgggaaaagg tgagtgagct atcagcgcgg attgactggt tacacgatga ttttactacc
     9661 gagcttaatt ctttagtgca ggatttcacc tggcagcagg gaacgctgct ggatcaaatc
     9721 gcctcccgac tgggcgatac ggcgcaatac ctgaagcgat ttcgtgaagt gcaaaatgaa
     9781 cagcagcagg tttataccct ggcacgcatt gaaaatcaga ttgttgacga tctgcgtgac
     9841 agactcaatg agctcaaatc aggacgtgat gacgacatac aggtggaaac tcatatccgt
     9901 tattttgaaa atctgaaaaa aacggcagat gaaaatatac gtatgctgga tgactggcct
     9961 ggcaccatta ccctgaggca gaccatcgat gaattgctgg atatgggaat cgtaaaaaac
    10021 aaaatgccgg ctacgatgcg tgaatatgtc gccgcccaaa aagccttagt ggatgccagt
    10081 cgcaccaggg aagcgacact gggtcgcttc agaacgttac tggaagcgca gcttggcagt
    10141 actcatcaac aaatgcagat gtttaatcaa cgaatggaac aaattgttcg cgttagcggt
    10201 gggttgatcc tggtggcaac agcactggcg ttactgctgg catgggtatt caatcattat
    10261 tttatccgct cacggttggt gaaacgcttt accctactga atcaggccgt tgtgcaaatt
    10321 ggtctgggag gcacggaaac ggctattcct gtttatggga atgatgaact ggggagaatt
    10381 gcaggattat tacgccatac tctcgggcag ctcaatgtgc aaaaacagca acttgaacaa
    10441 gaaattaccg atcgaaaggt gatagaagcc gatctgcgag ccacccagga cgaactgatt
    10501 cagacagcaa agttggcggt agtcgggcaa acgatgacca cgctggccca cgagatcaat
    10561 cagccgctaa atgcgctgtc gatgtatctg tttacagccc gcagggccat tgaacagacc
    10621 cagacagaac aggccagcat gatgcttggt aaagccgaag gggtgattag tcgtattgac
    10681 gccattatcc gttcactacg gcagtttacc cggcgcgccg aactggaaac atcactccat
    10741 gccgttgatt tagcacagat gttcagtgcg gcctgggaac ttctggccat gcgtcatcgc
    10801 cctttgcaag ctacgcttgt tctgccacag ggtacagcca cagtttcagg cgatgaggtc
    10861 agaacccagc aggtactggt taacgtactg gcgaatgcgc ttgatgtttg tggacaaggc
    10921 gctgtcatta ccgttaactg gcaaatgcag ggtaaaacac tgaacgtatt cattggcgat
    10981 aatggtccgg gctggcctga ggcattgttg ccttcgttat tgaagccgtt taccaccagt
    11041 aaagaagtag gactgggtat tggtctttca atttgtgtgt cgttgatgga gcaaatgaaa
    11101 ggggaattac gactggcatc aacgatgacc aggaatgcct gtgtggtact gcaattcaga
    11161 ctaacggatg tggaagatgc taagtagtga atattcgatt ctgctgattg atgatgatga
    11221 tgatgtgctg gacgcctaca cacagttact tgaacagtca ggctatcggg tttttgcctg
    11281 taacaatcct tttgaggcgc aggcgtggat acaaccagac tggccaggca ttgtgctaag
    11341 tgatgtgtgt atgcctggct gctctggtat cgatttgatg atgttattcc accaggatga
    11401 tcaacagatc cccattctgc ttatcaccgg gcacggcgac gtgccaatgg cagttgatgc
    11461 ggtaaaaaaa ggcgcgtggg attttttgca aaaaccggtt gatcccggca aattgctttc
    11521 tctggttgaa gaggcgctac gccagcgtca atcaatcatt gcgcgtcgtc agtattgcca
    11581 acagacattg caggtggagt tgatagggag aagtgaatgg ataaatcagt atcggcgacg
    11641 tttacagcag ttgtctgaga cagatattgc cgtctggctc tacggtgccc ctggcacagg
    11701 acggatgacc ggtgcacgtt atctgcacca atttggacgt aatgcacaag gcgaatttgt
    11761 ctaccgcgag ctgacaccag acaatgcacc acagcttaat gatttcattg cgctggctca
    11821 ggggggaaca ttagtgctta gccatcctga acacctgacg cgtgaacagc agtactatct
    11881 ggttcagtta caaagccagg aacacagacc ttttcgcctg attggtattg gagacacctc
    11941 gctggtcgag ctggccgcca gcaaccatat catcgcggag ctctattact gctttgcaat
    12001 gacacaaatc gcttgcctgc cattagctca acgccccgat gatattgaac cactgtttcg
    12061 ccattacctt tgtaaagcat gtcaacggct taaccatcct gttccggaag tgggaaaaga
    12121 gatgctcaaa gagatgatgc gccgcatgtg gccaaataat gtgcgagagc tggccaatgc
    12181 agcagaacta tttactgtag gagtattacc gctggcagaa acagcaaatc cgttggtgca
    12241 cgtcggaacc ccgacaccgc ttgacagacg agtggaggac gctgagcgac agataatcac
    12301 agaggcgctt aatatccatc aggggcgtat caatgaggtt gctgaatatt tacaaatccc
    12361 gcggaaaaaa ctctatctaa gaatgaaaaa atacggtctt agcaaggaac attataaaaa
    12421 ttaaatatca gtctctccag cacagaaatt atggagccca ccccagtctt ggtaacagtc
    12481 ccggggactg ctgtgagttt cttcaccgat tcagtaaatt tcaacaggga taaaatagtg
    12541 cagataatcg aagtattact ttcagagtgg ggaacatcca gagttttgtt cagaaagtgg
    12601 tggagcactg gctacatctg ttgatatttc actccactct ccactctcca ttcgactatg
    12661 agactacaat aactcagttc tcctggagct gattattctg gccagttttg taaccggagt
    12721 gtcatgtaca tgatccaaca gaaaatggcg tgacgctcaa tacttctcag gccaattact
    12781 atatgactca gcgtaagaac ggtataaccc agcatatctc ggtcatgaaa gcgggtatct
    12841 cagtccgttc tggtcgtcgg ttcgaaaaag gccagagagc aaaaaatagt gttcggcact
    12901 ggcgcacacg caaagatcct ctggaagcag tgtgggacag catgcttgtt cctctgttga
    12961 aagagaggcc tgctctgaca ccaacaactc tgctggagat gctacaggat aaatatcccg
    13021 gtcagtaccc caaaagcctt cgaagaacaa tgcaacggcg ggttcgcaaa tggaagctac
    13081 agtctggtgc agagcaggag gtcatgttcc gccagtggca tcagcccggt ctgcgaggcc
    13141 tgttggattt taccaaactg aaaggtgtgg tggtcaccat cgccggtaat ttgttggcgc
    13201 ataagttgta taacttccgt ctggaatgga gccactggag ctggatgcga gttgtgctgg
    13261 gtggtgagat cttctctgct ctggctgaag gtttgcagga agccctcgga caactgggtg
    13321 gagtgccggc agaacataaa acggacagcc tgagggcggc atggaaacaa caaggcaaaa
    13381 gatggacgct gcgagttgac tgagcgctat gctgctctct gtcagtacta cggaatgcag
    13441 ggcgtacacg ataataccag tcggggccac gaaaatggtt cggttgaaag tgcccacgga
    13501 catctgaaaa ggcgtatctg tcaggcactg atactgcggg gcagtaactc cttcagcacc
    13561 atagaagaat atcaggcctt catcactcag caggttatgc ggcacaaacg taaaagctga
    13621 atattaattg gggtaagt
//
LOCUS       DAZKHK010000128        13563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKHK010000128 DAZKHK010000000
VERSION     DAZKHK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.128"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..221)
                     /locus_tag="ACHOS5_004043"
     CDS             complement(<1..221)
                     /locus_tag="ACHOS5_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9778773.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGVLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKT"
     gene            complement(269..1151)
                     /locus_tag="ACHOS5_004044"
                     /pseudo
     CDS             complement(269..1151)
                     /locus_tag="ACHOS5_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(1249..1329)
                     /locus_tag="ACHOS5_004045"
     CDS             complement(1249..1329)
                     /locus_tag="ACHOS5_004045"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778774.1"
                     /translation="MQTRCVLQQVRDERLKDIGLCREDVV"
     gene            complement(1557..2477)
                     /locus_tag="ACHOS5_004046"
     CDS             complement(1557..2477)
                     /locus_tag="ACHOS5_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9778775.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPEREPYSWLKS"
     gene            2662..3942
                     /gene="yjiN"
                     /locus_tag="ACHOS5_004047"
     CDS             2662..3942
                     /gene="yjiN"
                     /locus_tag="ACHOS5_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP9778776.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            4058..5209
                     /gene="yjiM"
                     /locus_tag="ACHOS5_004048"
     CDS             4058..5209
                     /gene="yjiM"
                     /locus_tag="ACHOS5_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP9778777.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTAHVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            5219..5986
                     /gene="yjiL"
                     /locus_tag="ACHOS5_004049"
     CDS             5219..5986
                     /gene="yjiL"
                     /locus_tag="ACHOS5_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP9778778.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTSFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
     gene            5983..6240
                     /gene="ytiA"
                     /locus_tag="ACHOS5_004050"
     CDS             5983..6240
                     /gene="ytiA"
                     /locus_tag="ACHOS5_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP9778779.1"
                     /translation="MKEYLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGYTESVYCQQDGGWRCIAHYGISPR"
     gene            6305..7165
                     /gene="yjiK"
                     /locus_tag="ACHOS5_004051"
     CDS             6305..7165
                     /gene="yjiK"
                     /locus_tag="ACHOS5_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9778780.1"
                     /translation="MTKSISLSKRIFVIVILFVIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIHTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNLYIVSEPNR
                     FYRFTPQSSH"
     gene            7233..8411
                     /gene="yjiJ"
                     /locus_tag="ACHOS5_004052"
     CDS             7233..8411
                     /gene="yjiJ"
                     /locus_tag="ACHOS5_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9778781.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMMLIALTLLMPSKKHAITPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(8424..8978)
                     /gene="kptA"
                     /locus_tag="ACHOS5_004053"
     CDS             complement(8424..8978)
                     /gene="kptA"
                     /locus_tag="ACHOS5_004053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP9778782.1"
                     /translation="MAKYNDKELAETSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIAFIEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            9228..9911
                     /gene="yjiH"
                     /locus_tag="ACHOS5_004054"
     CDS             9228..9911
                     /gene="yjiH"
                     /locus_tag="ACHOS5_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP9778783.1"
                     /translation="MTQQGDAVAGELATEKVGVKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGTGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9908..10369
                     /gene="yjiG"
                     /locus_tag="ACHOS5_004055"
     CDS             9908..10369
                     /gene="yjiG"
                     /locus_tag="ACHOS5_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP9778784.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFAIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10382..11554
                     /gene="iadA"
                     /locus_tag="ACHOS5_004056"
     CDS             10382..11554
                     /gene="iadA"
                     /locus_tag="ACHOS5_004056"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9778785.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGITSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11619..12530
                     /gene="hypT"
                     /locus_tag="ACHOS5_004057"
                     /gene_synonym="qseD"
     CDS             11619..12530
                     /gene="hypT"
                     /locus_tag="ACHOS5_004057"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP9778786.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSPMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIEPG"
     gene            12537..12908
                     /locus_tag="ACHOS5_004058"
                     /pseudo
     CDS             12537..12908
                     /locus_tag="ACHOS5_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
     gene            complement(12956..13339)
                     /gene="iraD"
                     /locus_tag="ACHOS5_004059"
     CDS             complement(12956..13339)
                     /gene="iraD"
                     /locus_tag="ACHOS5_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP9778787.1"
                     /translation="MMRQSVQTVLPESTGNNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCYELAISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
BASE COUNT         3256 a         3424 c         3379 g         3504 t
ORIGIN      
        1 gtcttgttaa gagaatttgt cggaatcgca catactgcac ggcggaaaac ataagattca
       61 attaaacgaa ttatgctcag gaaatcttcg tgagacaaaa cgccattttt atagtcatga
      121 taaagcgcca gtaagaaagg atacgccaca tcgaccttta actcgcgcaa atcctgaaaa
      181 gccgtagcaa gcgatttgtc actttctttt cccaatgcca ttgcacagta atactccgcg
      241 tagatgtgaa tatcttcaac cagccgatct acaccttttt cagcaacact ctggctgcgg
      301 gcatggagtt taaatgcctc atagacttca tctgtccgag ggatctcccc cgtttttacc
      361 gtcagataat gacgcataaa actgtcaaaa tattcgctgt aaccttgctg accaaacgcg
      421 acttccattg gacgccagtg atcttcatac aaccgggttt gatgctctgg ttcgaggccc
      481 atcagaataa aattgcgcac cagatcggcc tgacttaatg ccttaccggt ggagttcata
      541 ctttcaaaaa tcagttgcgg attatcctga ccacgattaa gcgccacatc gacaattaat
      601 aactttgcta acccacgaca taaggggatc aagtcatcac cgagtttggt aatttgttca
      661 tcaaagaaag taaagttttc cattattcga tgggaatagt tttctggcat tggtctttgt
      721 tttattaacg ccagcaaact gtctttatca gtctcggtca gcagtaattt aaatcctttc
      781 tcgccggact cataaggatt gagtaaataa taattacgca atttcattgc tgaaaagcca
      841 tcaaaaactt cgtcttcgcc aagatgacgc gataaagcct caatcagcaa cattgcggtc
      901 gtcagccttt gttgaccatc aatgaccagt aacggagaaa taccagaaac ctgatacaac
      961 ccctgctcaa tataaacaac cgaaccgata aaatgcgctg atatatcgtc acgctttccg
     1021 gcacgaatga tgtcgtccca aagttgccga cattgttgtt ctgtccatga ataagtccgt
     1081 tgataaatgg gaataacgaa ctgctgcgaa cgttttaaaa aatcgagtag gcgggcttct
     1141 gtagctttca ttctgacgtc tttccgtgaa gcgatgattt cgcaataata accccttatc
     1201 tgacgaggtg acatataaat cccatcgttt cgtgaaggac tctctccatt acaccacatc
     1261 ctccctgcat aacccaatat cctttaaccg ttcatctctc acctgctgta acacgcaccg
     1321 ggtctgcatt tgctgccacc agcagcgtta atgtaccgtt cacataaatg gcggcggcaa
     1381 cgttggtgca ggaaggtcac tatcatcgcc acgtaaagtg tgcttcgttc atgccggatg
     1441 cggcgtgaac gccttatccg gcctacaaac cgcgcaaatt caatagattg cagagataat
     1501 gtaggtctga taagcgtagc gcatcaggca gctttgcgtt tgtcatccgt ctctgattag
     1561 ctcttgagcc atgaataagg ctcacgctca ggcaagggct gctctaatgc ctgtatttgc
     1621 tctgccgaaa gtccggtaat cttttgtatc cattcaggat ctaccccatt ctgcaaaaac
     1681 aatcgaagaa tgcgctgttc cccttttata tatccatcat tatgaattcg ctctgcaatc
     1741 gtcattattc gctccctttg ttgtggcagt cgacgggtaa tttcactgat aaactcatta
     1801 aaacgcgctt catcaccagt cagtaaaatg taatttaaca gcgcagttat ctggctgtca
     1861 ttagcacact ctgtaaccag taatactacc agttggtcaa taagccccat cagatcgcgc
     1921 tggcgaatat gcttttggat caactccaac agggcgactc tgcgatgctg cacaatctcg
     1981 tcgtctggca cgacagtgat atccaccagc gggaacgcgg cggtataaag cttccgtgcg
     2041 gtagttgggt cggcaaattc gtccagccag cacaaagacc agggataagg gctacggcta
     2101 ccgtgataaa acagcatcgg gatgaccagt ggtagctgcc ggcgtttatc atgttcgata
     2161 tggcgctgca tcaccgccat ggaatatcgc atcagacgaa aggccatatg aatatcctca
     2221 cggctctgat gttcaatcac tacataaata tagccatcgc cttcacgggt ctttaccgac
     2281 cacagaatat cggaatgtag cgcccgcaat ttttcatcga caaagctggc ggattccagt
     2341 tttaagctat cgagatcgca cagttcacgt aaatctttgg gtaagtggat ctccataaaa
     2401 tcccgcgcgg tgtcagagtg tgtgagaaag gatttaaata atgcgtcatg cggcgtgctg
     2461 gtcgtaaagt ttgtcatggc gatccgtcac ctgtagatca gatggcccaa ctttagcgac
     2521 taacaaagcc attcacacct ggctatttcc tattccccga ggcggtttcc aggctatttt
     2581 tattcttctg caaacgatac atttttatgg aagatatctc gctttttgcg ttttcttacc
     2641 gtcaggctct attcttacga tatgaataaa ctcattgaac tcagacgcgc caaaatgttg
     2701 gcgctctctt tactgcttat cgccgctgct acctttgtcg ttacgctgtt tttgccgccc
     2761 aatttttggg tgagcggtgt gaaggcgatt gctgaagcgg cgatggtcgg cgcgctggcg
     2821 gactggtttg cggtggtggc gctgtttcgc cgcgtgccga ttccgatcat ttctcgtcat
     2881 acggcgatta tcccgcgtaa taaagaccgg attggcgaaa atctcggcca gttcgtgcag
     2941 gaaaaatttc tcgataccca atcgctggtg gcattgattc gacgtcacga accggcgttg
     3001 ttgattggca actggtttag tcagccggag aacgcccgcc gcgttggtca gcatctgtta
     3061 cagatcatga gcggttttct cgaactgacc gatgatgcgc gtattcagcg cctgcttaag
     3121 cgtgcggtcc accgggcgat cgataaggtc gatctttccg gcaccagtgc gttgatgctg
     3181 gagagtatga ccagaaacga tcgtcatcag gtgctgctcg atacgctgat cgcacaactg
     3241 atcgccctgc ttcagcgcga taaatcacgc aagtttattg cccagcaaat tgttcgctgg
     3301 ctggagagcg agcatccact gaaagccaaa attttgccca ctgaatggct gggcgaacat
     3361 agcgcggagt tggtttctga cgcggtgaat tctttgcttg atgatatcag ccgcgatcgt
     3421 gcgcatcaga tccgccatgc gtttgatcgc gccacttttg ccctgatcga caagctgaaa
     3481 aacgatccgg aaatggcagc gcgagccgac gccgtaaaaa gctatctgaa agaagatgaa
     3541 gcttttaacc gctatctcag tgaattgtgg ggggatttac gggaatggct gaaagcggat
     3601 atcaacagtg aagattctcg tgtgaaagaa cgtatcgcgc gggcggggca atggtttggc
     3661 gaaacgttaa ttgccgatga tgccttgcgg gcgtcgttaa atggtcatct cgaacaagcc
     3721 gcacaccgcg ttgcgcctga gttttccgca ttcctgactc gccacattag cgatacggta
     3781 aaaagttggg acgcacggga tatgtcgcgg caaatcgagt taaatatcgg caaagatctc
     3841 cagtttatcc gtgtcaacgg tacgctggtt ggcggttgta ttggacttat tttgtatttg
     3901 ctgtcgcagc tcccggcctt gttccccctc ggcaatttat agaaatccat gaaaaactaa
     3961 gtggctaacc gcgagagaga tcaaataaac cacactaaaa gaatggtaat aatgtggcct
     4021 cttttcatta tctcccgtgg tacggggaag gaaaatcatg tcacttgtta ccgatctacc
     4081 cgccattttc gatcagttct ctgaagctcg ccagaaaggc tttctcaccg tcatggatct
     4141 caaggagcgc ggcattccgc tggttggcac ctactgcacc tttatgccgc aagagatccc
     4201 gatggcagcc ggtgcggttg tggtttcgct atgttccacc tctgatgaaa ccattgaaga
     4261 agccgagaaa gatctgccgc gcaacctctg cccgctgatt aaaagcagct acggcttcgg
     4321 caaaaccgat aaatgcccct acttctactt ttctgatctg gtggtcggtg aaaccacctg
     4381 cgacggcaaa aagaaaatgt atgaatacat ggcggaattt aagcccgttc atgtgatgca
     4441 actgccaaac agcgttaagg acgatgcctc acgcgcgtta tggaaagccg agatgctgcg
     4501 cttgcaaaaa acggtagaag aacgttttgg gcacgagatt agcgaagatg ctctgcgcga
     4561 tgccattgcg ctgaaaaacc gcgaacgtcg cgcactggcc aacttttatc atttggggca
     4621 gttaaatcct ccggcgctta gcggcagcga cattctgaaa gtagtctacg gcgcaacctt
     4681 ccggttcgat aaagaggcgt tgatcaatga actggatgca atgaccgccc acgttcgtca
     4741 gcagtgggaa gaaggccagc gactggaccc gcgcccacgt attttaatca ccggctgccc
     4801 aattggcggc gcagcagaga aagtggtgcg cgcgattgaa gagaatggcg gctgggttgt
     4861 cggttatgaa aactgcaccg gggcgaaagc caccgagcaa tgcgtggcgg aaacgggtga
     4921 tgtgtacgac gcgctggcgg ataaatatct ggcgattggc tgctcctgtg tttcgccgaa
     4981 cgatcagcgc ctgcaaatgc tcagccagat ggttgaagaa tatcaggtcg atggcgtagt
     5041 tgatgtgatt ttgcaggcgt gccataccta cgcggtggaa tcgctggcga ttaaacgtca
     5101 tgtgcgccag cagcacaaca ttccttatat cgctattgaa acagactact ccacctcgga
     5161 tgtcgggcag ctcagcaccc gtgtcgcggc ctttattgag atgctgtaag gagtggcagt
     5221 gacatattcg attggcattg attccggctc aaccgccacc aaagggatct tactggctga
     5281 cggcgtgatt acgcgccgtt tcctcgttcc aacatccttt cgcccggcaa cagcaattac
     5341 tgaagcctgg gaaactctgc gcgaagggtt agagacaacg ccgtttctga cgctcaccgg
     5401 ctacggacgg caactggtgg attttgccga taaacaggtg acggaaatct cctgtcacgg
     5461 gctgggcgca cggtttcttg agccagcaac gcgcgcggta atcgacatcg gtggtcagga
     5521 cagcaaagtg attcagcttg atgatgacgg taacctgtgc gatttcctga tgaatgacaa
     5581 atgcgcggcg ggcaccgggc gtttcctgga ggtgatctcg cgcacgcttg gcaccagcgt
     5641 cgagcaactc gacagcatta ccgaaaatgt cataccgcac gccatcacga gtatgtgcac
     5701 agtgtttgct gaatcagaag tgatcagcct gcgctctgcg ggcgtagcgc cagaagcgat
     5761 tctcgccgga gtgattaacg cgatggcgcg gcggagcgcc aatttcattg ctcgtctctc
     5821 ctgtgaagcg ccgattctgt ttactggtgg cgttagccac tgtcaaaggt ttacccacat
     5881 gctggaaagc catctgggaa tgccggtaca aactcaccct gatgcgcaat tcgctggtgc
     5941 aattggcgcg gcggtgatcg gtcagcggca gagaaaacgc gcatgaaaga gtatctgttt
     6001 ttatttcact ccacggtcgg cgtcatacaa acccgcaagg cgttgcaggc agcgggcatg
     6061 acttttcgcg tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg
     6121 ctgacctgtc cgcctggcga ggaaattcaa tgggtgatcc ctgggtatac ggaatcagtt
     6181 tattgtcagc aggatggtgg ctggcgctgc attgcacatt acgggatttc tccgcgttaa
     6241 ggattcatta agatcgctac gtatacttct cgatatctta ttgatttgcc ttaaagggat
     6301 taccgtgaca aaaagtattt cgttaagtaa acgcatcttc gttattgtga ttctttttgt
     6361 cattgtggca gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca atcatgcagc
     6421 atcatttcag aattaccacg ccaccattga cggcaaagaa attgcaggca taacgaataa
     6481 tatctcttcg ctgacctggt cggcacaaag caatactctt tttagcacta tcaacaaacc
     6541 ggccgccatt gttgaaatga ccaccaatgg cgatttgatc cacacaattc ctctggattt
     6601 tgttaaggat ctcgagacga tcgaatacat tggcgataat cagtttgtca ttagcgatga
     6661 gcgcgattat gccatttatg tgatttcact gaccccaaac tcggaagtaa aaatcctcaa
     6721 aaaaataaaa atcccgctgc aggaatcccc aaccaattgc ggttttgaag gattggccta
     6781 ttctcgccag gatcacactt tctggttttt taaagagaaa aacccgattg aggtctacaa
     6841 agttaacggg ctgttaagta gcaatgaatt gcacatcagc aaagacaagg cactacaacg
     6901 gcaatttact ttagatgatg tgtccggcgc agaatttaac caacaaaaaa atacgcttct
     6961 ggtgctgtct catgaatctc gcgcactcca ggaagtaacg ctggttggcg aggttatcgg
     7021 tgaaatgtca ctaaccaaag gcagtcgagg tttgtcgcat aacatcaaac aggccgaagg
     7081 ggttgcgatg gatgcctctg gtaatcttta tatcgttagc gaaccaaatc gtttttatcg
     7141 ctttacccct caatcatcgc attaacaggc tggcatgttt tacctttttg ttattaactt
     7201 gttaacaaca aaaatgacaa acaaggttaa acatgccttc atccacgcat cccgtagaac
     7261 gcttttcttt cagcaccgcg cttttcggga tgctggttct gaccttaggt atgggtttag
     7321 gccgcttcct ttatacgcct atgttgcccg tcatgatggc ggaaggatcg ttttcattta
     7381 gccagctctc gtggattgcc agcggcaact atgcggggta tctggctggc agtctgctat
     7441 tttcgtttgg cgcatttcac cagccatcgc gcctgcgccc atttctgttg gcttctgccc
     7501 tggcgagcgg gttgttgatc ctcgcaatgg catggttgct gccatttatt ctggtgttat
     7561 tgattcgcgt cctggcgggt gtcgccagcg ccggaatgct gatttttggt tcgacactga
     7621 ttatgcagca cacgcgccat ccttttgtgc tggcagcttt gttttctggc gttggcattg
     7681 gcatcgcact gggcaatgaa tatgttctgg caggcctgca ttttgacctc tcctcgcaaa
     7741 cgttatggca aggcgccggg gcgctttctg gcatgatgct gattgcactt acgcttttaa
     7801 tgccctcgaa aaaacacgcc atcacaccaa tgccattggc aaaaacggag caacagataa
     7861 tgagctggtg gttactggct attctgtacg gcctggcggg atttggttat atcatcgtcg
     7921 cgacttacct gccgctcatg gcgaaagatg caggctcacc attgttaacc gcccatctct
     7981 ggacgttagt cggcttatca attgtgcctg gttgctttgg ctggctatgg gcagcaaaac
     8041 gttggggggc tttgccctgc ctgacggcga atttgctggt gcaggctatc tgtgtgctgc
     8101 ttactctcgc cagcgactcg cctctcttgc ttatcatcag cagtcttggt tttggcggta
     8161 cctttatggg cacgacatct ctggtgatga ctatcgcccg ccaactgagc gtaccgggaa
     8221 atctcaatct tttaggcttt gtgacgctca tttacggcat tgggcaaatc ctcggcccgg
     8281 cgctgaccag tatgctcggc aatggaacat cggcgcttgc cagcgccact ctctgcggcg
     8341 cggcggcgtt gtttattgct gcgcttatca gcacagtgca attatttaag ctacaagtgg
     8401 tcacttcatg agagaagttt cccttaccac tcaagaaact caaccgcaac tgttgatgtc
     8461 agccagaccc cgttttccgc ttgccagaaa gggatccctc gtttcgccat ttcctgtgct
     8521 ttgacggtta agataaccgg cgaaccgtgt cttgctccca ctttgcgtgc cgttgcttca
     8581 tcagcagaga gatgcacgta atggcgctcc cctgcaatca gcccttgttt ttttatctcg
     8641 tcaagaaaac ggcttgccgt accatggtag agaaattgtg gcggtgtttt ttcgatgaag
     8701 gcaatggcga cctgcgaagt cgaatgcccc tgaacggcgc gaatacacgt accatcactg
     8761 gaataactaa agcgcttctt atcgctggtg gcgaccacag tatccagtaa tgcgcgggtt
     8821 aaccgcttgc ccgctttttg tgcacagagt atgagcttat cgatatccgc ccatccttca
     8881 cggtccagca cgatgccaat cgcctctggt ttatgtcgga ggacaaaact taaaaacttg
     8941 ctcgtctctg ccagctcttt gtcgttgtat tttgccatcg cgttttcgtc cctggatttt
     9001 ctgtttttat ttactgatgt cattgtcact gggtttatga attttctgca actcaatcct
     9061 gcgcagaacc tttgccacac agcgtcattt ttccgctatg caaaaaacgc atagcaagaa
     9121 acatacttgc attttcccca tacccacatg gctgataagg tttttcagct ccaggaattt
     9181 tcctggctca agcaacacac atacacaaaa aaacaatggg gattgttatg acacaacaag
     9241 gggatgctgt agctggggag ctggcgacag aaaaagtggg tgttaaaggg tatctggcct
     9301 tttttctcac gattatcttt ttttccggag ttttttccgg gacagacagt tggtggcgcg
     9361 tttttgactt ttccgttctg aacggatcgt ttgggcaact gcctggtgcg aatggcgcta
     9421 ccacctcgtt tcgcggcgca ggtggcacag gagctaaaga tggctttctc ttcgcactgg
     9481 aactggctcc gtcagtgatt ctctctctgg gaattatctc gattaccgat ggtcttggcg
     9541 gattgcgcgc cgcgcagcag ttaatgacgc ccgtgctaaa accgttgttg ggcattccag
     9601 gcatctgttc actggcgctg attgctaacc tgcaaaacac cgatgccgcc gcaggtatga
     9661 ccaaagaact ggcgcaggaa ggggaaatta ccgaacgtga taaagtgata ttcgccgcct
     9721 atcaaaccag cggtagcgcc atcatcacca actacttctc ttccggcgtc gcggtgtttg
     9781 cctttctggg cacgtccgta atcgtacctt tagccgtcat cctggtgttt aaatttgtcg
     9841 gagccaacat tttgcgcgtc tggcttaact tcgaagaacg tcgcaaccca acgcaaggag
     9901 cgcaagcatg acaactcagg tccgtaaaaa cgtcatggat atgtttattg atggcgcgcg
     9961 ccgtggcttt actatcgcta ccaccaacct gctgccaaac gtggtgatgg cctttgccat
    10021 catccaggcg ctgaaaatta ctggcctgct cgactgggtg ggacatatct gtgaacccgt
    10081 aatggcgctg tggggattgc ccggtgaagc ggcaaccgtc cttttagccg cattgatgag
    10141 catgggcggc gcagtcggtg ttgccgccag tctggcaacc gctggcgcat taaccggcca
    10201 cgatgtcacc gttttactgc ccgcgatgta tttaatgggc aacccggtgc aaaatgtcgg
    10261 tcgctgcctg gggacagccg aagtgaatgc caaatattat ccgcatatca ttacggtctg
    10321 cgtgattaac gcattgctgt cgatctgggt tatgcagctt attgtttaac aaggagttac
    10381 catgattgat tattccgcag ccggttttac cctgctgcag ggagcgcatt tgtatgcgcc
    10441 ggaagatcgg ggaatttgcg atgtcctcat cgctaacggc aaaattattg ccgttgccag
    10501 caacatccct tctgacattg taccggactg cacggttgtc gatctcagcg ggcagatcct
    10561 ctgcccaggt tttattgatc aacacgtcca tttgattggt ggtggcggcg aagcaggtcc
    10621 cacgacacgc acgccggaag tggcgttaag tcgtctgacg gaagcgggca tcacgtcagt
    10681 ggttggcctg ctgggtaccg actctatctc tcgccacccg gaatccctgc tcgccaagac
    10741 ccgtgcgctc aatgaagaag gcatcagcgc ctggatgctg accggcgctt atcatgtccc
    10801 ttcccgcact attacgggtt ccgtggaaaa agacgtggcg attatcgatc gcgtgattgg
    10861 tgtgaaatgc gccatctctg atcaccgttc tgccgcaccg gacgtttatc acctggctaa
    10921 tatggcggcg gaatcccgcg ttggcggttt gctcggcggt aaacctggcg tcaccgtgtt
    10981 ccacatgggt gacagcaaaa aggcgttaca gcctgtctat gacctgctgg aaaactgcga
    11041 tgtgccgatc agcaagttgc tgccgaccca cgttaaccgc aacgttccgt tgtttgagca
    11101 ggcgctggag ttcgcgcgca aaggcggcac catcgacatc accagcagca ttgacgaacc
    11161 agtcgcccct gccgaaggta ttgcccgcgc cgttcaggcg ggtattccgc tggcacgcgt
    11221 caccctcagc tccgacggca acggtagcca gccgttcttt gatgacgaag gaaatttaac
    11281 ccatatcggt gttgcaggtt ttgaaacatt gctggaaacc gtgcaggtgc tggtcaaaga
    11341 ctatgatttc agtatcagcg atgccctgcg cccgctcacc agtagcgtag ccggtttcct
    11401 taacctgacc gggaaaggcg aaattctgcc aggcaatgac gcagacttac tggtcatgac
    11461 gcctgaattg cgcattgagc aggtatacgc tcgcggcaaa ctgatggtca aagacggcaa
    11521 agcctgcgtg aaaggaacgt ttgaaacggc ataaacggtt gtcagccgcc cgaatggacg
    11581 tagaatgccc gcaagggcgg ctgacagagt ataacgtaat ggatgactgt ggtgcggttt
    11641 tgcataacat tgaaaccaaa tggctttatg attttctgac cctggaaaaa tgtcgcaatt
    11701 tttcccaggc ggcagtcagt cgcaacgtct cgcaaccagc attcagccgc cgcatccgtg
    11761 cgctggaaca ggcgattggt gttgaattgt ttaaccgcca ggtaacgccg ctgcaactct
    11821 cagaacaagg gaaaatcttc cattcgcaga tccgccatct gttgcaacag ttagagagca
    11881 atctggcaga gctgcgtggc ggcagcgatt acgcgcaacg taaaatcaag atagccgctg
    11941 cacactctct ttccctcggt ctgttaccgt ccattatcag ccagatgccg ccgctcttta
    12001 cctgggcaat tgaagccatt gatgtcgatg aagcggtcga taaactgcgc gaagggcaaa
    12061 gtgactgtat tttttccttt cacgacgaag atttgctgga agcgccgttt gaccacatcc
    12121 gcttatttga atctcaattg ttccccgtct gcgccagtga cgaacacgga gaagcacttt
    12181 ttaacctcgc tcagccacac tttccgttac tgaattacag ccgcaactcc tacatggggc
    12241 gattgattaa tcgcactctg acgcgccaca gtgagttgag tttcagcacc ttttttgtct
    12301 ctccgatgag cgagctttta aagcaggttg ccctcgacgg atgtggaatt gcctggttgc
    12361 cggagtatgc catccaacaa gaaattcgca gcgggcagct cgttgtactg aatcgggacg
    12421 aactggtgat cccgattcag gcctatgcat accggatgaa tacccgcatg aatccagttg
    12481 ccgaacgctt ctggcgtgaa ctgcgcgagc tggagattga acctggctga aagttttctg
    12541 cagaaaatct tccggcgctg ggaattatga ttgaggttcc tgccgcaatc atgattgcag
    12601 aaaaattagc cagtgaagtc gatttctttt ccatcggcac caacgatctg acgcaatata
    12661 ttatggcggc agacagagga aatagcaccg tcgcgaaact ggtcgattat tgtaatgacg
    12721 cggtgattaa cgctattgct atggtgtgtc aggccggacg taataatgaa attccggtca
    12781 gtatgtgcgg cgaaatggct ggagatatcc agcaaacagc acgactgctc accatgggga
    12841 ttgataagct cagtgccagt ccatcccggc ttccggcctt gaaagcagca atcagaacct
    12901 cccactaatt gtgacctaac gcgatacgtc ttgcgcgtat cgcgtttccc gtcatttact
    12961 gcggcatcgt gcgatgccag ctgcccttct gccagaccag gtcaaacgtt agcggctcat
    13021 catcgtggta cagcatcatc tccagactaa tggccaactc gtagcaatag gcttctttca
    13081 gattgacctg caatgccgca atacgtggct catgaaataa aagcgcggaa cgtaactggc
    13141 ggcaatacca actctggaga agcttgtcgc tctgattatg cccttgccac tcaggcatgc
    13201 ctgaaacaag taatattggt agcggtgaat ggggagaact aaaaagttga aagagatccc
    13261 ggcatacgga atcacgcagc gataacgtat tgtttcctgt actttcaggt aataccgtct
    13321 gaactgattg gcgcatcatt ttgcgcactc ctgacgttta gtggcatctg tatcctgccc
    13381 tggctgccgg acaccactgg gttgatgctc atattccata atcattcctt tatgctcatt
    13441 gcgtccctgg cactttcata agggatttat aagatccgcc aaagataaaa cactgtacag
    13501 aaaagaatat ccactgactt ttaatttaaa aaggtatcat ttgacaaaag tcattaaaca
    13561 taa
//
LOCUS       DAZKHK010000129        13484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKHK010000129 DAZKHK010000000
VERSION     DAZKHK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.129"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(79..231)
                     /locus_tag="ACHOS5_004060"
     CDS             complement(79..231)
                     /locus_tag="ACHOS5_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9778788.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(496..1230)
                     /gene="cysH"
                     /locus_tag="ACHOS5_004061"
     CDS             complement(496..1230)
                     /gene="cysH"
                     /locus_tag="ACHOS5_004061"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9778789.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHOS5_004062"
     CDS             complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHOS5_004062"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP9778790.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDKIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHOS5_004063"
     CDS             complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHOS5_004063"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP9778791.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQPAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYYVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5134..5496
                     /gene="queD"
                     /locus_tag="ACHOS5_004064"
     CDS             5134..5496
                     /gene="queD"
                     /locus_tag="ACHOS5_004064"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP9778792.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVLQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5574..6845
                     /locus_tag="ACHOS5_004065"
     CDS             5574..6845
                     /locus_tag="ACHOS5_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9778793.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLIWRHLRRHGLWHLTGDVIRSIRCL"
     gene            6836..7096
                     /locus_tag="ACHOS5_004066"
     CDS             6836..7096
                     /locus_tag="ACHOS5_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9778794.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GNLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7113..7688
                     /locus_tag="ACHOS5_004067"
     CDS             7113..7688
                     /locus_tag="ACHOS5_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP9778795.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7836..8696)
                     /locus_tag="ACHOS5_004068"
     CDS             complement(7836..8696)
                     /locus_tag="ACHOS5_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFP9778796.1"
                     /translation="MNIVLVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMIAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELATRLAWRVHGASICQVISFDISTASVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGVEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(8693..9472)
                     /locus_tag="ACHOS5_004069"
     CDS             complement(8693..9472)
                     /locus_tag="ACHOS5_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP9778797.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEITRETVAAEAPA
                     IQCLQLARPEQRRGATLIDGQTVAEKAQKLWQYYLCQRMQP"
     gene            complement(9450..10859)
                     /locus_tag="ACHOS5_004070"
     CDS             complement(9450..10859)
                     /locus_tag="ACHOS5_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9778798.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     RFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNAGGANEHSVSV"
     gene            complement(10881..12335)
                     /locus_tag="ACHOS5_004071"
     CDS             complement(10881..12335)
                     /locus_tag="ACHOS5_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP9778799.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRMAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12405..13190)
                     /locus_tag="ACHOS5_004072"
     CDS             complement(12405..13190)
                     /locus_tag="ACHOS5_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9778800.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
BASE COUNT         3168 a         3660 c         3391 g         3265 t
ORIGIN      
        1 aacaatataa attaaaaaag ggtgcaggag accaaaccat cacaattcag tacgcggggt
       61 tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat
      121 accacgttct ctgatactca actcacacaa cgagtcgcct accatcagcg tgaatcccag
      181 tactgtaaaa cacagcacta tgattgccac aagggcatat tttgtcagca tgtcttgcct
      241 ccctgcaaag aagagactaa catccgaatt gcttaggttc gaacgtaggc ctcaggttga
      301 tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca
      361 gcctcaagca cccaatgcca ttctatacct gttaattctc cctgcgacgt tctcgtttaa
      421 atgccggaat gctgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa
      481 tttaagcagc aatatttacc cttcgtgtaa tccacattcc ctttttaagc caaagaaacg
      541 cgtttcttct tccgccatgc cgggttccca tttacgggtt gtatgggtat cgcccaccga
      601 taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca ggtactgata
      661 aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc
      721 cagcaccggt aaattggcgc gactgccgga ttgttcacgg cgcaggccag caaaccaggt
      781 ttgcgcattc agttctttca gcgcccggtt catcggttcg actttgttga tgtcattgta
      841 cttttcaatg ccttcaacgc cctgctccca tagttttccg taacgtgctt cctgccaggc
      901 ggcgctttcg gtagcacggt acactttcag gttgagcttg agtttgtccg ttaactcgtc
      961 aataaagcgg taggtttccg ggaacagata gccggtatcg gtgaggatca ctggaatatc
     1021 cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa agctggaaga
     1081 aagtacatat tcaccgggca gattatccag cgcccaggct acgcggcctt cagcgtccag
     1141 tttttccagt tgggcgttag tttccgccag cgccagaatg cgatctacct tcggcagttc
     1201 gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc
     1261 gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc cataaatcgc
     1321 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
     1381 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
     1441 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc
     1501 caagatgcag gttgtagcga cccggcgctt tacccaccag acccacttcc gccagcatcg
     1561 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
     1621 catgtttcgc cattaaatta tcgattttgt cgataaaaga cggcaggaaa cgctcggctt
     1681 cggccatcgc cagcgggcaa gtcgggaatg acacgcaggc cattgagttt tcacgctgcg
     1741 gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc gctttctcgc
     1801 tttccggcac accggcgatg atcagattct ggttcgccgt aatgcggaaa tcacctttgt
     1861 ggatcttcgc gatctccagc aagccggttt tcagcggacg tcccggataa tcaagaatgc
     1921 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
     1981 tacgatctcc gcgtccggta aactcatacg ggcgaatcgg ctcgaatttg atccccgcgc
     2041 gacgttcaac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg tatttggttt
     2101 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg acggcttccg
     2161 ccaccgccag cgtatgctcc agcggcagat agccaaactc actcgccgtg cgggcgtagg
     2221 ttttcttgtt gccgtgttcg atggaaagcc caccgcccac cagcaggtta aagcccacca
     2281 gcttgccgtt ttcggcgatc gccacgaagt tcatgtcgtt ggcgtgcaga tcgatatcgt
     2341 tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc tgaccaagga
     2401 tcggctcttc atcagtagtg gcgacttttt cctgatcgag ccagatctcc gcatacgcgc
     2461 gggtacgagg cagcagatgt tcagaaattt tcttcgccca ttcatatgct tccgcatgca
     2521 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttggcggtcg
     2581 ccagcgcatc aagaccgacc gagtgcagca tctgatgcac cggtttgacg ttctttttca
     2641 gaatgccgtg gaactggaat gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
     2701 tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc ccacccggca
     2761 gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc
     2821 gaatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttatcgc
     2881 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
     2941 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
     3001 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
     3061 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
     3121 tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc
     3181 gtcattgatc cagcgccaca gctctgcgcc ctgttcgcgc agtttgtctt gtacgtaaat
     3241 tttttctttt tgatcgcgcg accaggcgag atcgatgcgc gtcagcacgc cttctttgac
     3301 gtaacgctgc cattccacct ggtagaggaa atcttccgta aagtgtgggt tgccaaagaa
     3361 tagccagttt ttacccggcg cttcgtcggc agcacgctgc tgcataaagg cacggaacgg
     3421 cgcgatgccg gtgccagggc caatcatgat caccggggtt tccggattgg ttggcaggcg
     3481 gaagttgtcg ttatgttcga taaagacgcg gacttcaccc tcttcttcca cacgatccgc
     3541 gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtagtaac gcaccacacc
     3601 aacggtaacg tgcacttcgt tctcgacttc cgcctgcgag gaggcgatgg aatacaggcg
     3661 cggggtcagc gggcgcagca gattaatcag cgcttcggca tccagttgcg ccggagagaa
     3721 acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
     3781 caccagcggc agcagcgttt cgctgcgggt aagcgtggca tagttctcaa caatgttggc
     3841 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
     3901 ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt ctttcaccag
     3961 tgccggatcg ttctgatacc agacgcccaa cgcgtcaccc ggctggtaac gcaggccaga
     4021 gtcacctaag tcaatttcaa tgtgacgaac gtctttttca gagttacgcc cggtaatttt
     4081 ctggttaaca gagaggctcg ccaccagcgg cgcgtctttg ctatacgggc tggtgtggat
     4141 ttcatttacc gtgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
     4201 aagcgcatca accacgcggg cgcgccactc gctggcagca ggctggtatt caacgtcggc
     4261 atcgacacgg tcgagcaggc gttcaccgcc cagttccgcc agcttactgt caaaatcttt
     4321 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaacgcggt
     4381 gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
     4441 cggcggttcc ccttcccctt gcgttgacgt caccacgata agcagttttt cgctggcgat
     4501 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ccgctaataa
     4561 atcatcgcgt aatgcttcag caacccgacg cgcattgcca gtttgcgagg cggagataat
     4621 agttataccc ggcatttctg cggcgggcgc tggcgtcgct gcaagcgcag caggctgctg
     4681 gttgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtaggag ttaaatcggt
     4741 cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg
     4801 gacctgtgtc gtcatgcgtt gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
     4861 gcgcccataa aacagaagag aaggtaaggt taacggggta aacggtgtgg attaaagacg
     4921 ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca
     4981 aaatagatta accaacccaa tgaaaaacaa atgaatattg cccatcaata agataaatca
     5041 gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct
     5101 acggcggttt tatcgtcatg tagagaaatt atgatgtcca ccacgttatt taaagatttc
     5161 accttcgaag ccgctcaccg cttgccgcac gtcctacaag ggcataaatg tggtcgcctg
     5221 cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc
     5281 tggattatcg atttcgctga actgaaagcg gcgtttaaac caacttatga gcgcctcgat
     5341 caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaagt tttagcaaaa
     5401 tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa
     5461 acctgcaccg caggttgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt
     5521 cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat gacatggaag
     5581 acgactgcga cattattatt atcggtgccg gtattgctgg caccgcttgc gcgttacgct
     5641 gcgcgcgagc gggtttatcc gttctgttac tggaacgagc ggagattccc ggcagcaaaa
     5701 atctttccgg cgggcgttta tatacccatg cgctcgcgga actcctcccg caatttcacc
     5761 tgaccgcgcc ccttgaacga cgcatcactc acgaaagtct ttctctgtta acgccagatg
     5821 gcgcaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac
     5881 gattcgatcc gtggctggtt gccgaagccg aaaaagaagg tgtcgaatgc atccccggtg
     5941 cgacggtaga tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg
     6001 atattcttcg cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctcgcggaac
     6061 gccacgggtt agtgactcgt cctgctggcg aagcgatggc attggggatc aaagaagtgc
     6121 tgacgctgga accatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag
     6181 cgttgttgtt cagcggcggg atctgtgatg acttacccgg cggcgcattt ctttatacta
     6241 atcaacaaac gctctcgtta gggattgttt gcccgctctc ctcccttacg caaagtcgtg
     6301 ttccggcaag cgagctgctg gctcgcttta aaacacatcc ggcagtgcgc ccgcttatca
     6361 aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc ctgcgcagta
     6421 tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg
     6481 tcaataccgg aatttccgtg cgcggtatgg atatggcgct aactggcgcg caggcggcgg
     6541 cacaaacgct gataagcgcc tgccagcacc gcgagccgca aaatctgttt ccgctttatc
     6601 atcacaacgt agagcgcagc ctgctgtggg atgttctaca gcgttatcag catgttccgg
     6661 cgcttttgca acgccctggc tggtatcggg tgtggcctgg gttaatgcag gatatttccc
     6721 gtgatttatg ggatcagggg aatacacctg ttccaccgct gcgccagttg atctggcgtc
     6781 atttacgtcg tcacggcttg tggcatctga cgggcgatgt tatcaggagt attcgatgtc
     6841 tgtagcccgt aatctctggc gcgctgctga tgcgccgcac attgttccgg ctgactccgt
     6901 tgagcgccag acggcacaac ggttgattaa cgcttgtccg gctggtcttt tttcgctcac
     6961 acctgaaggt aacttacgcg ttgattatca cggctgcctg gagtgtggca cctgccgttt
     7021 gctgtgcgac gaatcaacac tacaacagtg gcgctatccg ccttccggat tcggcatcac
     7081 ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg ctccgccaga
     7141 atccggtgat tgctgccgta aaagataatg ccagcctgca actggcaatc gactctgaat
     7201 gccagtttat ttccgtgttg tacggcaata tctgcaccat tagtaatatc gttaaaaaga
     7261 ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata
     7321 aagaagtggt gattcagttt ttgaaactgg tgaccgaggc ggacggaatt atcagtacca
     7381 aagcctcaat gctgaaagca gcaagagcgg aaggtttttt ctgtattcat cgactgttta
     7441 ttgttgattc gatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt
     7501 gtattgagat cctgccaggc tgcatgccta aagtgctggg ctgggtgacg gagaaaatcc
     7561 gccaaccgct gattgctggc gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta
     7621 acgcgggtgt tgtggcgctt tccaccacga ataccggggt ctggacgtta gcgaaaaaat
     7681 tgctttgacg ggataatcgt aacaagttga atttggtgtg atttgtaggc tgcacgccac
     7741 atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta caagtcccca
     7801 gcccagaacc ataggcagga tcatccggca gcacgttatt ggcaatctgc gtgaatggtt
     7861 gtcaccagtg cctccagcac cgctttccag tcatcaacca cgccaacatc tgcctgtgaa
     7921 aataccgccg cgctggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc
     7981 gccattaacg ctgctgcgcc ggaagcgcca acaacaatac acacctcagg tgccagcaga
     8041 tgtccggaaa taccaatcac tttttcggca tcaacgccac cgttcatcac tcgtgcccga
     8101 ctgtagccca cttcgactcc catcttttct gccagcatag cgatttcctg gttatctgcc
     8161 tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc acgggtaaca
     8221 tttttcagat tttcgacgct gataagccag tctggaagcg cgccggggac aatgttcagt
     8281 tgctgcatcc cagagggcaa cgtggcgttt ttatctgctc ccgcctgacg tgccagcgaa
     8341 aggcatagcg ggcgtttttc tgtttgcaac gtcgccgtta gcgcatttcc ccagtgagat
     8401 ttgcgcacgc ttgcggttga tatatcgaat gaaatcacct ggcagatact ggcgccatgt
     8461 acacgccagg ccagtcgggt agccagttca tcgccaaacg cgcccggagg aaagaggaca
     8521 acctccgcag gcgttcgttg ccactgttcc accagcgcat ccagcacctg ttccgcaatc
     8581 atcggctgtg gttctatttg ccagtgtgcc agggtacaac cgctgaaatc ctgcgccgcc
     8641 agccagctcg ctattgcggc atggtcctga tttattgtca caagtacgat attcatggct
     8701 gcatcctttg gcataaatag tactgccata gcttttgcgc tttttctgct accgtttgcc
     8761 catcaatcag cgtcgctccg cgtcgttgct ctggccttgc cagttgcagg cactgtatcg
     8821 ctggcgcttc cgcggcaaca gtttcgcggg taatctccgc tttccccgcc gccattcgct
     8881 ggcgcattcc cggtactggc aaggccactt caccacactg acgcacggca atcaccgcag
     8941 gcaagcgaac ccggcaacag cgcagtccat gttcagtacg ctgttcgagg gtgataaacg
     9001 gcgcgtcgag agtaaagcgc tccacctggg tgaagcaggg ccagaccagc atttccgcca
     9061 gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccagta attatcagat
     9121 ccagcggatt ctggtactgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc
     9181 gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagtgcc atgagatagc
     9241 gcagccagtg caacgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac
     9301 cgttttttct ctgcgccaac agcagcgcgg cggcagcctg ttcatcagca ccgagtaaac
     9361 ttcgcagtag cgaaacatcc ggtccgctat taccctgagc cgccgcctgc cactcttttt
     9421 ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct
     9481 cctgcatttc ccgccgccac cagcgggagt gctttggttt ctggtgccca tagccaggtc
     9541 acaacaaaac caaccaacaa cactgccgcc aggagcaata aggtggcttg cattccccac
     9601 tgcgccagca cccacggcag caggccagta cttaccgccg cccccaaccg actcatggcg
     9661 gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg
     9721 cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt
     9781 aatgaactgc cggaaggcaa acaggccatc actaccagcg ttgccgccag cagcaaaaaa
     9841 cttcccagta aaaatctgcg atgcgctaac aagtgggtca acactaaccc taataacgcg
     9901 ccaacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata
     9961 gtttgggcga tggtcggcag ccaggtataa atcacaaacc atgggattac gaggcagaca
    10021 aagaagacgc tgttaaacgc cgtgcgccgc cagtagcgcg aagagaacaa ggttttgatg
    10081 tgtttatggg tcgccgttgc cacttcatcg cccagtaaaa catgcgggcc aaaatagcga
    10141 tgaacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggcgattcc
    10201 ggcgtgcccc aacgtagcag cgtaatcagc aaggctggta acgctgctga tgccagcagc
    10261 cagcgccagg cctccgggct ttcggaaata aagtgatgtc cggcaatact tgccagcaca
    10321 tagcctacgg tccacaccac actgaatgcg cccagcaaaa taccgcgatg gcggcgcggg
    10381 gaaaattcag ccagcaaggt gtgaccgact gagtaatcgc ctcccagccc aatgccgatc
    10441 aaaatgcgca atccaataag atgctctggc gtggtggcaa aaaactgtaa aaacgaagca
    10501 agcgtaatca gcataaaact gaaggtgaag attttttgcc gcccgatatg gtcggagatc
    10561 catccaagaa ccaggctgcc aagaaacaaa ccaaggagtg ctgagccgcc aatcattccc
    10621 gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta accaatgacg
    10681 ccgaggacat aaccgtcggt caggtgcgca ccgaaagtga gcgcagcaat gcggcagtga
    10741 aaacggttaa gtggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca
    10801 ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccgtcatc
    10861 gaggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt attcataatg
    10921 ccgttaggat cgaactgctt tttcagccct tccagcaacg tccacgcgct gccgtgttcc
    10981 agtttgctcc agtgaacgcg atgtttacca ataccgtggt ggtgcaccat cgaaccgccg
    11041 agacgaatgg tttcttcgca gatgattttg ttgagcggat tgtggtattt gtcgatttcc
    11101 tcttccggct tacagtcaac gacgttgtag tcgtagacga agtacatgtt ggtgccgttc
    11161 tggtagctgt gagaggagtg accgcccagc atggtgatgt cgtcggcgtg cgggaactcg
    11221 gtacgaatac ggttaataac gctttcgtag atttcgtgaa tgcagctcca gcagccggac
    11281 acttcggtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagctact
    11341 ttatccggtc cccagttcag gttgttaaac caggtttcga tcagcttgct gtccacgcgc
    11401 tggcattgcg ggtaacgtgc cacgatttca gcaatccctt cgcccgttgc cttcgccatg
    11461 cgaggattcc cctcagccat aaagatcagc acgcatttgc cgtcggcaaa atgggtgaag
    11521 tgttgggtgc catcttcagc atcatacaaa cgagcaatcg acggacgata cccttccacc
    11581 atgatttcac gcaggatgtt gaagccggtt ttcatatctt ccaggatata gccgtagaag
    11641 agattgtttt ccggcgtaaa tttgaagatt ttcactgtga cttcagtgat atagcacagt
    11701 gcaccttcgt tgccgatgat gatatgacga atgtccgggc cagccgcgcg acgtggcacg
    11761 tttttaatgc gtgtgacggt gccatctgcc agcaccgctt ccagaccaac gaccatatcc
    11821 tcgattgcgc cgtagagtgt ggaaaactgc ccgatactgc gggtcgccac caggccgccc
    11881 atctgcgcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac
    11941 gcgttttcca gcacctccag cggaacgcca cattgcgccg tggcctgcat attctcaata
    12001 tcaatattaa tgatttgatt catggcggag ccgtcgagca ccaccgagtt ttccacaacg
    12061 gtttccagcc caccttcggt ggcggaagca ccggtacgtg gcacaccgtt aattttgtgc
    12121 gcattcataa aattcagcac acgggatact tgttctgtgg aaccgagttt tacgaccgct
    12181 gccggaattg gcaaagtata aatgccatga atatccggaa atttacgaaa acggtcaata
    12241 ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac aatttccttt
    12301 agctggtcga caatcgctac gcgagataaa gacatagtaa ttccttcctg ataataaaaa
    12361 tggaatattc attaataaga gaaaacagaa accaccgtta tgaattagcg cactaaataa
    12421 ccgccatcaa ccactaataa atgtccgttg acataattcg atgccggact tgcgaggaat
    12481 acggctgcac ccattaaatc ctgagtatca ccccaacggt ttgccggaat atgatcaaga
    12541 acacgctggt tagtttctgg attactacgg gtcgccagcg taatatcggt tgcataataa
    12601 ccaggagcaa taccattcac ctgaatatta tattggccta attcatcgca ataggctttg
    12661 gtgaatccgg caagagcatg tttagtggca gaataagcag gtgaccattg accacctaag
    12721 taagagaaca atgaacagat attaatgatt ttaccgcttt tctgcgggat cataattttt
    12781 gccgcttcgt agcttaactc gaatgcggcg gtcaagttca cgtcaatcat cgggtcccag
    12841 tcggcacgac caaagtccag caccttattc agcttacaaa taccggcatt gttaaccaga
    12901 atatctacgg taccgaagcg ctcacagcag gcagcgataa tcttctgcgg cgcgccttct
    12961 gcggtgatat ccacctgcat aaaatcaact tcgacaccct gtttttcaat catttcctta
    13021 gtttcgccgt tatctttgac gaaactagga ataaagacat ttgcgccagc tttggccaac
    13081 gccatggcaa atgcctggcc taaaccgcta ttcccgccgg taacaattgc tgttttacct
    13141 ttcagggaga aaaaatccat tgagaacgca ttgagagatt cgattgacat agttagctcc
    13201 aatttccgta aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga agttaccttt
    13261 ctcattatct ctggtaactg gagataattt ttagcataag gacataactg ctaatgtgat
    13321 cgtaatcaca gtgtgatgtt cgtattgtca aataatttaa aaaagcagaa gcgtgattac
    13381 tctcacacaa cacctctgct ttccatgtta tttcttattg caattactag agacctgaga
    13441 aaagtaatcc acctgaaagg gcggcttact tttctctttt tttt
//
LOCUS       DAZKHK010000130        13419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKHK010000130 DAZKHK010000000
VERSION     DAZKHK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.130"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..427
                     /locus_tag="ACHOS5_004073"
     CDS             <1..427
                     /locus_tag="ACHOS5_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFP9778801.1"
                     /translation="QEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDL
                     VPDFARRLAAKLGLPFIDAIEKVVDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPAL
                     LVDDIVDSAWTLTVLTALLRQAGCPTVYPLALASTSVKN"
     gene            334..1629
                     /locus_tag="ACHOS5_004074"
     CDS             334..1629
                     /locus_tag="ACHOS5_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP9778802.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEVGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPMQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVTPAHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1675..2268)
                     /gene="yhgN"
                     /locus_tag="ACHOS5_004075"
     CDS             complement(1675..2268)
                     /gene="yhgN"
                     /locus_tag="ACHOS5_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP9778803.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2460..3563
                     /gene="asd"
                     /locus_tag="ACHOS5_004076"
     CDS             2460..3563
                     /gene="asd"
                     /locus_tag="ACHOS5_004076"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9778804.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3836..6022
                     /gene="glgB"
                     /locus_tag="ACHOS5_004077"
     CDS             3836..6022
                     /gene="glgB"
                     /locus_tag="ACHOS5_004077"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP9778805.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            6019..7992
                     /gene="glgX"
                     /locus_tag="ACHOS5_004078"
     CDS             6019..7992
                     /gene="glgX"
                     /locus_tag="ACHOS5_004078"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP9778806.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNASIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPALSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGVFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            8010..9305
                     /gene="glgC"
                     /locus_tag="ACHOS5_004079"
     CDS             8010..9305
                     /gene="glgC"
                     /locus_tag="ACHOS5_004079"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP9778807.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9305..10738
                     /gene="glgA"
                     /locus_tag="ACHOS5_004080"
     CDS             9305..10738
                     /gene="glgA"
                     /locus_tag="ACHOS5_004080"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP9778808.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10757..13204
                     /gene="glgP"
                     /locus_tag="ACHOS5_004081"
     CDS             10757..13204
                     /gene="glgP"
                     /locus_tag="ACHOS5_004081"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP9778809.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3176 a         3316 c         3656 g         3271 t
ORIGIN      
        1 ccaggagaaa aaaacggggc gcttcagtga tgagctggtg gaggcatgtg cggaaatggt
       61 tcgccaacgc tggaatccgc atcctgaacc cacctgggta tgctgcgttc cttcattaag
      121 gcacctcgac ctggttcctg attttgcccg gcgactggcg gcgaaacttg gcttaccttt
      181 tattgatgcc attgaaaaag tcgtggacaa tccaccgcag aaaatgcagc aaaaccgttt
      241 tcaccagtgt caaaatctcg acggggcgtt tgtgattacc cctcctttga tgccaggtcc
      301 ggcgttgctg gttgacgata tcgtggattc tgcatggacg ctgacagttc tgacagcact
      361 gttacgccag gcaggttgcc cgacggttta tcctcttgcc cttgcttcta cctcggtaaa
      421 aaattgatga atctttcagc caatgcacaa gcaactctcc tgctaaccag cgatttttct
      481 cgcgcggcgg cgagtgagta taaacctctt agtaatagtg aatgggggaa gtttgcatta
      541 tggctgaagc accaacgtat cagtcccgcc gagcttctgg tgccgcaacc gcaagagaaa
      601 cttacaggct ggagcgatcc gcgtatttct caggagcgta ttcttggctt gctggcgcgt
      661 ggtcatagtc tggcgttggc ggtagataag tggcaacgcg ccggtttatg gatcttaacc
      721 cgcggagatg ccgattatcc cgttcgcttg aaaaaccgat tgcgaacgga tgcacctccc
      781 gttttatttg gctgcgggaa taaagcatta ctgcaagcgg aaggtatggc gattgttggc
      841 tcgcgagatg ctccgactga cgatttgcgc tatacccaac aactggccgc gaaactggcc
      901 caacagggga tttgcgttat ctctggtggt gcgcgaggta ttgatgaatg tgcaatggcc
      961 tcggctctgg aggtcggggg aactgccgtt ggcgtattag ctgatagctt gttaaaaacg
     1021 agtacgttag tgaaatggcg tgaagggctt atagcaggca acctggtgtt gatttcgccg
     1081 ttttacccag aggtacgttt caccgtcggc aatgcgatgg cgcggaataa atatatttat
     1141 tgccttgctg aaagcgcaat ggttgtacgt gcgggaatga ccggtggaac gataaccggg
     1201 gcgatggagg cattaaaaca tcagtggctg cccatgcagg ttaaaccaaa tcaggatatg
     1261 caatcagcca attcacgatt agtagaaaat ggggcgtcat ggagtgctga acaggctgag
     1321 aatgtgacga tcagactgcc agacgttcct gggctgatgt atgacagagc actccgtaac
     1381 gctcaaccag aactgttttc gctgcacgaa gatgacgcaa attacgcagt aacgcccgcg
     1441 catacccctg tcgattttta tcaactcttt gtggcggaac tggcgatcct tgcaaaggaa
     1501 tcgataagta ttgaaaggct ggcgtcttgt actggtttaa ccatcgaaca aattagtgtg
     1561 tggctgaacc gcgcagaaga agagggaagg gttatccgat tgggtgaagg tcattatcag
     1621 ttcaggtaat aaaacaggtg ccgcgtctgg ttgtgtgcaa cacctgtttg aaccttaccc
     1681 cttcatccac attcgaatgc cgtcgaggaa catctgagtt gccatcatca ccagaatcaa
     1741 tcccatcagg cgttcaagtg cgttcacccc tttctcgccc agcagacgta aaaatagcga
     1801 agactgtagc aggatgacaa aggtgccgcc ccaggccagc agcagagcaa tcaccagatg
     1861 tcccatctga ttcgggtact gatgagacaa caacatcagc gtggcgagaa tagtcggccc
     1921 ggcgaccagc ggaattgcca acggcacgat aaatggctct tcacctgccg gaagcccgct
     1981 gctatttcct gaagcgctgg ggaaaatcat cttaatggcg atcagaaaca gaatgatgcc
     2041 gccagaaatg gagacggttt ctgcccgcag gctaagaaat gccagaattt tctcgcccgc
     2101 aaacaggaac accagcatca caaggagagc aataagcaac tctcgcacca tgattgcccg
     2161 ccgtcttttc ggttcagtat gtttcagtac ggacatgaaa ataggtaggt ttccgagcgg
     2221 atccataatc aggatcaata aaactgttgc agaaatgatt tcattcataa ctcaaattcc
     2281 ctgataattg ccgcggactt tctgcgtgct aacaaagcag gataagtcgc attactgatg
     2341 gcttcgctat cattgattaa tttcacttgc gactttggct gctttttgta tggtgaaaga
     2401 tgtgccagga ggagaccggc acatttatac agcacacatc tttgcaggaa aaaacgctta
     2461 tgaaaaatgt tggttttatc ggctggcgcg gtatggtcgg ctccgttctc atgcaacgca
     2521 tggttgaaga gcgcgacttc gacgccattc gccctgtctt cttttctact tctcagcttg
     2581 gtcaggctgc gccgtctttt ggcggaacca ctggcacact tcaggatgcc tttgatctgg
     2641 aggcgctaaa ggccctcgat atcattgtga cctgtcaggg cggcgattat accaacgaaa
     2701 tctatccaaa gcttcgtgaa agcggatggc aaggttactg gattgacgca gcatcatctc
     2761 tgcgcatgaa agatgacgcc atcatcattc ttgaccccgt caatcaggac gtcattaccg
     2821 acggattaaa taatggcatc aggacttttg ttggcggtaa ctgtaccgta agcctgatgt
     2881 tgatgtcgct gggtggttta ttcgccaatg atcttgttga ttgggtgtcc gttgcaacct
     2941 accaggccgc ttccggcggt ggtgcgcgac atatgcgtga gttattaacc caaatgggcc
     3001 atctgtatgg ccatgtggca gatgaactcg cgaacccgtc ctctgctatt ctcgatatcg
     3061 aacgcaaagt cacaacctta acccgtagcg gtgagctgcc ggtagataac tttggcgtgc
     3121 cgctggcggg tagcctgatt ccgtggatcg acaaacagct tgataacggt cagagccgcg
     3181 aagagtggaa agggcaggcg gaaaccaaca agatcctcaa cacatcttcc gtaattccgg
     3241 tagatggttt atgtgtgcgt gtcggggcat tgcgctgcca cagccaggca ttcactatta
     3301 aattgaaaaa agatgtgtct attccgaccg tggaagaact gctggctgcg cacaatccgt
     3361 gggcgaaagt tgttccgaac gatcgggaaa tcactatgcg tgagctaacc ccagctgccg
     3421 ttaccggcac gctgaccacg ccggtaggcc gtctgcgtaa gctgaatatg ggaccagagt
     3481 tcctgtcagc ctttaccgtg ggcgaccagc tgctgtgggg ggccgcggag ccgctgcgtc
     3541 ggatgcttcg tcaactggcg taatctttat tcattaaatc tggggcgcga tgccgcccct
     3601 gctagtgcgt aatacaggag taagcgcaga tgtttcatga tttaccggga gttaaataga
     3661 gcattggcta ttctttaagg gtggcggaat acacgagtat tcacagcctt acctgaagtg
     3721 aggacgacgc agagaggatg cacagagtgc tgcgccgttc aggtcaaaaa aatgtcacaa
     3781 ccagaagtca aaaattcagt tggatggggt gacacaataa aacaggaaga caagcatgtc
     3841 cgatcgtatc gatagagacg tgattaacgc gctaattgca ggccattttg cggatccttt
     3901 ttccgtactg gggatgcata aaaccaccgc gggactggaa gtccgtgccc ttttacccga
     3961 cgctaccgat gtgtgggtga ttgaaccgaa aaccgggcgc aaactcgcaa aactggagtg
     4021 tctcgactca cggggattct ttagcggtgt cattccgcga cgtaagaatt ttttccgcta
     4081 tcagttggct gttgtctggc atggtcagca aaacctgatt gatgatcctt accgttttgg
     4141 cccgctaatc caggaaatgg atgcctggct attatctgaa ggtactcacc tacgtccgta
     4201 tgaaacctta ggcgcgcatg cagatactat ggatggcgtc acaggtacgc gtttttctgt
     4261 ctgggctcca aacgcccgtc gggtctcggt ggttgggcaa ttcaactact gggacggtcg
     4321 ccgtcatccg atgcgactgc gtaaagagag cggaatctgg gaactgttta tccccggggc
     4381 gcataacggt cagctctata aatacgagat gattgatgcc aatggcaact tgcgcctgaa
     4441 gtccgaccct tatgccttcg aagcgcaaat gcgcccggaa accgcgtctc ttatttgcgg
     4501 gctgccggaa aaggttgtgc agactgaaga gcgcaaaaaa gcgaatcagt ttgatgcgcc
     4561 aatctctatt tatgaagttc acctgggctc ctggcgtcgc cacaccgaca acaatttctg
     4621 gttgagctac cgcgagctgg ccgatcaact ggtgccttat gctaaatgga tgggctttac
     4681 ccatctcgag ctactgccca ttaacgagca tcccttcgat ggcagttggg gttatcagcc
     4741 aaccggcctg tatgcaccga cccgccgttt tggtactcgc gacgacttcc gttatttcat
     4801 tgatgccgca cacgcagctg gtctgaacgt gattctcgac tgggtgccag gccacttccc
     4861 gaccgatgac tttgcgcttg ccgaatttga tggcacgaac ttgtatgaac acagcgatcc
     4921 gcgcgaaggc tatcatcagg actggaacac gctgatctac aactatggtc gccgcgaagt
     4981 cagtaacttc cttgtcggta acgcacttta ctggattgaa cgttttggta ttgatgcgct
     5041 gcgcgtcgat gcggtggcgt caatgattta tcgcgactac agccgtaaag agggggagtg
     5101 gatcccgaac gaatttggcg gtcgcgagaa tcttgaagcg attgaattct tgcgtaatac
     5161 caaccgtatt cttggtgagc aggtttccgg tgcagtgaca atggcggagg agtctaccga
     5221 tttccctggc gtttctcgtc cgcaggatat gggcggtctg ggcttctggt acaagtggaa
     5281 cctcggctgg atgcatgaca ccctggacta catgaagctc gacccgattt atcgtcagta
     5341 tcatcacgat aaactgacct tcgggatgct ctacaactac actgaaaact tcgtcctgcc
     5401 gttgtcgcat gatgaagtgg tccacggtaa aaaatcgatt ctcgaccgga tgccgggcga
     5461 cgcatggcag aaattcgcta acctgcgcgc ctactacggc tggatgtggg cattcccggg
     5521 caagaaacta ctgttcatgg ggaacgaatt tgcccagggc cgcgagtgga accatgacgc
     5581 cagcctcgac tggcatctgt tggaaggcgg cgataactgg caccacggtg tccagcgtct
     5641 ggtgcgcgat ctgaaccaca cctaccgcca ccataaagca atgcatgaac tggattttga
     5701 cccgtacggc tttgaatggc tggtggtgga tgacaaagaa cgctcggtgc tgatctttgt
     5761 gcgtcgcgat aaagagggta acgaaatcat cgttgccagt aactttacac cggtgccgcg
     5821 tcatgattat cgcttcggta ttaaccagcc gggtaaatgg cgtgaaatcc tcaataccga
     5881 ttccatgcac tatcacggca gtaatgcagg caatggcggc gcagtacaca gcgatgagat
     5941 tgccagccac ggtcgtcagc attcactgag cctgacgcta ccacctctgg ccactatctg
     6001 gctggttcgg gaggcagaat gacacaactc gccattggca aacccactcc cctcggcgcg
     6061 cattacgacg gtcagggcgt caacttcaca cttttctccg ctcatgccga gcgggtagag
     6121 ctgtgtgtct ttgatgccaa tggccaggaa catcgctatg acttgccagg gcacagtggc
     6181 gacatttggc acggttatct gccggatgcg cgcccgggtt tgcgttatgg ttatcgcgtt
     6241 catggcccct ggcaacccgc cgaggggcat cgctttaacc cggcgaagtt gttgattgat
     6301 ccttgcgcgc ggcaaattga cggggagttt aaagataacc cgctgctgca cgccggtcat
     6361 aatgaacctg actatcgcga caacgcctcc attgcgccga aatgcgtagt ggtggttgat
     6421 cactatgact gggaggatga tgccccgccg cgcacgccgt ggggcagcac catcatttat
     6481 gaagcccatg tcaaaggatt aacgtatctg cacccggaga tcccggtcga gatccgtggc
     6541 acttataaag ccctggggca tccggtgatg atcaactatt tgaaacagtt gggcattacc
     6601 gcgctggaac tgctgccggt ggcacaattt gccagcgaac cacgtctgca acgtatgggg
     6661 cttagtaact actggggtta caacccggtg gcgatgtttg cgctgcatcc ggcgtatgcc
     6721 tgctcgccag aaacggcgct ggatgagttt cgcgatgcaa tcaaagcact gcataaagcg
     6781 ggtatcgaag tcattcttga tatcgtgctc aaccatagtg cggaactgga cctcgacggc
     6841 ccgttattct cgctgcgtgg gatcgacaac cgtagctatt attggataag agaagacggc
     6901 gattatcaca actggaccgg ttgtggtaac acgctcaatt tgagtcatcc ggcggtggta
     6961 gattatgcca gcgcctgcct gcgttattgg gtagaaacct gccacgtcga tggtttccgc
     7021 tttgatctgg cggcagtcat gggccgtacg ccagagtttc gtcaggatgc gccgttgttt
     7081 accgctatcc agaactgccc ggcgctctcg caggtgaagt taattgctga accgtgggat
     7141 atcgctcctg gtggttatca ggtgggaaat ttcccgccgc tgtttgccga gtggaacgat
     7201 catttccgcg atgcagcccg tcgtttctgg ctgcattatg atttacctct gggagtgttt
     7261 gccgggcgtt ttgctgcctc cagcgatgtt tttaaacgta atggtcgtct gccgagtgcc
     7321 gcgattaatc tcgtcaccgc gcatgacggt tttacgcttc gcgactgcgt ttgcttcaac
     7381 cataaacaca atgaagcaaa cggagaagaa aatcgcgacg ggaccaacaa caattacagt
     7441 aacaatcatg gtaaagaagg attaggcggt actcttgatc tggttgaacg gcggcgcgac
     7501 agcattcacg ccctgttaac aacgttgttg ctctcccagg gtacgccgat gttactggcc
     7561 ggtgacgaac atggtcacag ccagcatggc aataacaatg cctactgtca ggataaccaa
     7621 ttaacctggt tggactggtc gcaggcaagc agtggtttaa ccgcatttac cgccgcgtta
     7681 atccatctgc gcaagcgcat tcccgctttg atggagaatc gctggtggga agaaggcgac
     7741 ggcaatgtcc gttggctaaa tcgatatgct caacctttaa gcacggatga gtggcaaaac
     7801 gggccgaaac agctgcaaat tctgctctcg gatcgctttt tgatcgcaat taacgccacg
     7861 cttgaggtaa cagagattgt tttacctgct ggggagtggc acgccattcc cccattcgct
     7921 ggagaggata acccagtgat tacggctgtc tggcagggac ctgcacacgg attgtgtgtg
     7981 ttccagagat gataaaaaag gagttagtca tggttagttt agagaagaac gatcacttaa
     8041 tgttggcgcg ccagctgcca ttgaaatctg ttgccctgat actggcggga ggacgtggta
     8101 cccgcctgaa ggatttaacc aataagcgag caaaaccggc cgtacacttc ggcggtaagt
     8161 tccgcattat cgactttgcg ctgtctaact gcatcaactc cgggatccgt cgtatgggcg
     8221 tgatcaccca gtaccagtcc cacactctgg tgcagcacat tcagcgcggc tggtcattct
     8281 tcaatgaaga aatgaacgag tttgtcgatc tgctgccagc acagcagaga atgaaagggg
     8341 aaaactggta tcgcggcacc gcagatgcag ttacccaaaa cctcgacatt atccgccgtt
     8401 ataaagcgga atacgtggtg atcctggcgg gcgaccatat ctacaagcaa gactactcgc
     8461 gtatgcttat cgaccacgtc gaaaaaggtg cacgttgcac cgttgcttgt atgccagtac
     8521 cgattgaaga agcctccgcg tttggcgtta tggcggttga tgagaacgat aaaattatcg
     8581 aattcgttga aaaaccagct aacccgccgt caatgccgaa cgatccgagc aaatctctgg
     8641 cgagtatggg tatttacgtc tttgacgccg actatctgta tgaactgctg gaagaagacg
     8701 atcgcgatga gaactccagc cacgactttg gcaaagattt gattcccaag atcaccgaag
     8761 ccggtctggc ctatgcgcac ccgttcccgc tctcttgcgt acaatccgac ccggatgccg
     8821 agccgtactg gcgcgatgtg ggtacgctgg aagcttactg gaaagcgaac ctcgatctgg
     8881 cctctgtggt gccggaactg gatatgtacg atcgcaattg gccaattcgc acctacaatg
     8941 aatcattacc gccagcgaaa ttcgtgcagg atcgctccgg tagccacggg atgaccctta
     9001 actcactggt atccggcggt tgtgtgatct ccggttcggt ggtggtgcag tccgttctgt
     9061 tctcgcgcgt tcgcgtgaat tcattctgca acattgattc cgccgtattg ttaccggaag
     9121 tatgggtagg tcgctcgtgc cgtctgcgcc gctgcgtcat cgatcgtgct tgtgttattc
     9181 cggaaggcat ggtgattggt gaaaacgcag aggaagatgc acgtcgtttc tatcgttcag
     9241 aagaaggcat cgtgctggta acgcgcgaaa tgctacggaa gttagggcat aaacaggagc
     9301 gataatgcag gttttacatg tatgttcaga gatgttcccg ctgcttaaaa ccggcggtct
     9361 ggctgatgtt attggggcat tacccgcagc acaaatcgca gacggcgttg acgctcgcgt
     9421 actgttgcct gcatttcccg atattcgccg tggcgtgacc gatgcgcagg tagtatcccg
     9481 tcgtgatacc ttcgccggac atatcacgct tttgttcggt cattacaacg gggttggcat
     9541 atacctgatt gacgcgccgc atctctatga tcgtccggga agcccgtatc acgataccaa
     9601 cttatttgcc tataccgaca acgtattgcg ttttgcgctg ctggggtggg ttggggcaga
     9661 aatggccagc gggcttgacc cattctggcg tcctgatgtg gtgcatgcgc acgactggca
     9721 tgcaggcctt gcgcctgcgt atctggcggc gcgcgggcgt ccggcgaagt cggtgtttac
     9781 tgtgcacaac ctggcctatc aaggcatgtt ttatgcacat cacatgaatg acatccaatt
     9841 gccatggtca ttctttaata ttcatgggct ggaattcaac ggacaaatct ctttcctgaa
     9901 ggccggtctg tactatgccg atcacattac ggcagtcagc ccaacctacg ctcgcgagat
     9961 caccgaaccg cagtttgcct acggtatgga aggtctgttg caacagcgtc accgtgaagg
    10021 gcgtctttcc ggcgtactga acggcgtgga cgagaaaatc tggagtccag agacggactt
    10081 actgttggcc tcgcgttaca cccgcgatac gttggaagat aaagcggaaa ataagcgcca
    10141 gttacaaatc gcaatggggc ttaaggttga cgataaagtg ccgctttttg cagtggtgag
    10201 tcgtcttacc agccagaaag gtctcgacct ggtgctggaa gccttacctg gtcttctgga
    10261 gcagggcggg cagctggcgc tactcggcgc gggcgatccg gtgctgcagg aaggtttcct
    10321 tgcggcggca gcggaatacc ccggccaggt gggcgttcag attggctatc acgaagcatt
    10381 ttcgcatcgc attatgggcg gcgcggacgt cattctggtg cccagccgtt ttgaaccgtg
    10441 cggcttaacg caactttatg gattgaagta cggtacgctg ccgttagtgc ggcgcaccgg
    10501 tgggcttgct gatacggttt ctgactgttc tcttgagaac cttgcagatg gcgtcgccag
    10561 tgggtttgtc tttgaagata gtaatgcctg gtcgctgtta cgggctattc gacgtgcttt
    10621 tgtactgtgg tcccgtcctt cactgtggcg gtttgtgcaa cgtcaggcta tggcaatgga
    10681 ttttagctgg caggtcgcgg cgaagtcgta ccgtgagctt tactatcgct tgaaatagtt
    10741 ttcaggaaac gcctatatga atgctccgtt tacatattca tcgcccacgc ttagcgtaga
    10801 agctcttaag cactctatcg cttacaagct gatgtttacg attggcaagg atccggtcgt
    10861 cgccaataaa catgaatggc tgaacgcaac gttatttgct gtgcgcgatc gtctcgtgga
    10921 gcgctggtta cgctcaaacc gtgcccagtt gtcgcaagaa actcgtcagg tttactacct
    10981 gtcgatggag tttttgattg gccgtacgct ttccaacgcc atgttgtcgc taggaattta
    11041 cgaagatgta cagggcgcac tggaagcgat gggattaaat ctcgaagagc tgattgatga
    11101 agaaaatgac ccgggcctcg gtaacggtgg cctgggccgt ctggcggctt gcttcctcga
    11161 ttctctggcg acgttagggt tgccggggcg cggttacggc atccgctatg actacggtat
    11221 gttcaagcag aacatcgtta acggtagcca gaaagagtcg cctgactact ggctggaata
    11281 cggtaacccg tgggaattca aacgccacaa cacgcgctat aaagtccgtt ttggcggtcg
    11341 cattcagcag gaaggtaaaa aaacgcgctg gattgaaacc gaagagattc tgggagtagc
    11401 ttacgatcag ataatcccag gttacgacac cgacgcgacc aacacgttgc gtttgtggag
    11461 tgcccaagcc agtagcgaaa ttaacctcgg taaattcaac cagggcgact acttcgcggc
    11521 agtggaagat aaaaaccact ccgagaacgt atctcgcgta ctgtatccgg acgactccac
    11581 ctactccggg cgtgagctac gactgcgtca ggaatacttc ctggtttcct cgaccattca
    11641 ggacatttta agccgccatt atcagttgca taaaacctac gataacctgg cggataaaat
    11701 tgcgattcac ctcaacgata ctcatccggt gctgtcgatt cctgagatga tgcgtctgct
    11761 gatcgatgag caccaattta gctgggacga cgcgtttgaa gtgtgttgtc aggtcttctc
    11821 ctacaccaac cacacgctaa tgagcgaggc gctggaaacc tggccagtcg atatgctggg
    11881 taaaattctg ccgcgtcacc tgcagatcat ctttgaaatc aacgactatt tcctgaaaac
    11941 cttgcaggaa cagtatccga acgataccga tctgctggga cgggcgtcga tcattgatga
    12001 atccaacggt cgtcgtgtgc gtatggcctg gctggcggtt gtggtgagcc acaaagttaa
    12061 cggtgtatcg gagctgcact ctaacctgat ggtgcaatcg ctatttgccg actttgcgaa
    12121 aatcttcccg ggtcgtttca ccaacgtcac caacggtgtg acgccgcgtc gctggctggc
    12181 ggtagcgaac ccatcgcttt cagccgtgtt ggacgaacac ctgggccgca actggcgcac
    12241 cgaccttagc ctgcttaatg atctgcaaca acactgtgat ttcccaatgg ttaatcacgc
    12301 ggtgcatcag gcgaagctgg agaacaaaaa gcgtctggcg gagtatatcg cccagcaact
    12361 gaatgtggtg gtaaatccga aagcgctgtt cgatgtgcaa atcaaacgta ttcacgaata
    12421 caaacgtcaa ttgatgaatg tgttgcacgt gatcacccgc tataaccgca tcaaggccga
    12481 cccggatgcg aagtgggtac cgcgcgtgaa tatttttggc ggtaaggcgg cttcggccta
    12541 ttacatggcg aagcacatta ttcatttgat caatgacgta gcgaaagtga tcaacaacga
    12601 tccgcagatt ggcgacaagc tgaaagtcgt gttcatcccg aactacagtg ttagcctggc
    12661 gcagttgatc attccggcgg ccgatctgtc tgaacagatt tcgctggcgg ggacggaagc
    12721 ttccggcacc agtaacatga agtttgcgct taacggcgcg ctgactatcg gtacgctgga
    12781 cggtgcgaat gtcgagatgc tggatcatgt cggtgctgac aatatcttta tctttggtaa
    12841 cacagcggaa gaagtggaag aactgcgtcg tcagggctac aaaccgcgtg aatactacga
    12901 gaaagatgag gagctgcatc aggtgctgac gcaaatcggc agcggtgtat tcagtccgga
    12961 agatccgggt cgctatcgcg atctggttga ttcgctgatc aacttcggcg atcactacca
    13021 ggtactggcg gattatcgca gttatgtcga ttgtcaggat aaagtcgacg aactctacga
    13081 gcgtcaggaa gagtggaccg caaaagcgat gctgaacatt gccaatatgg gctacttctc
    13141 ttctgaccgt acgatcaaag agtacgccga tcatatctgg catattgatc cggtgagatt
    13201 gtaagttcaa atacggctaa ataccgtgtg ttccgctgaa gtaatacaga gtggaactca
    13261 cggtatttct atacacaata aacaataact ttgatgtatg ttattctatt taatatatac
    13321 cattaatata tattaaataa cgattgatga taatgttttt ggtattttgt tatttcaaat
    13381 aatattaatt tattatgaat gcattaatat tattcatgt
//
LOCUS       DAZKHK010000131        13316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKHK010000131 DAZKHK010000000
VERSION     DAZKHK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.132"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..216)
                     /gene="ydfK"
                     /locus_tag="ACHOS5_004082"
     CDS             complement(<1..216)
                     /gene="ydfK"
                     /locus_tag="ACHOS5_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFP9778810.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQN"
     gene            complement(285..398)
                     /locus_tag="ACHOS5_004083"
     CDS             complement(285..398)
                     /locus_tag="ACHOS5_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFP9778811.1"
                     /translation="MNSILIITSLLIIFSIFGHALIKLGIGISNNPDKTDV"
     gene            complement(825..1073)
                     /locus_tag="ACHOS5_004084"
     CDS             complement(825..1073)
                     /locus_tag="ACHOS5_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9778812.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            1293..2879
                     /gene="prfC"
                     /locus_tag="ACHOS5_004085"
     CDS             1293..2879
                     /gene="prfC"
                     /locus_tag="ACHOS5_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9778813.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            3272..3877
                     /gene="osmY"
                     /locus_tag="ACHOS5_004086"
     CDS             3272..3877
                     /gene="osmY"
                     /locus_tag="ACHOS5_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP9778814.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            4004..4165
                     /locus_tag="ACHOS5_004087"
     CDS             4004..4165
                     /locus_tag="ACHOS5_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP9778815.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            4287..5360
                     /locus_tag="ACHOS5_004088"
     CDS             4287..5360
                     /locus_tag="ACHOS5_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP9778816.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            5357..6136
                     /locus_tag="ACHOS5_004089"
     CDS             5357..6136
                     /locus_tag="ACHOS5_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9778817.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDAIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(6353..7216)
                     /locus_tag="ACHOS5_004090"
     CDS             complement(6353..7216)
                     /locus_tag="ACHOS5_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP9778818.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAACEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(7188..8738)
                     /locus_tag="ACHOS5_004091"
     CDS             complement(7188..8738)
                     /locus_tag="ACHOS5_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP9778819.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPAL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            8996..9775
                     /gene="deoC"
                     /locus_tag="ACHOS5_004092"
     CDS             8996..9775
                     /gene="deoC"
                     /locus_tag="ACHOS5_004092"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9778820.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            9853..11175
                     /gene="deoA"
                     /locus_tag="ACHOS5_004093"
     CDS             9853..11175
                     /gene="deoA"
                     /locus_tag="ACHOS5_004093"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP9778821.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            11227..12450
                     /gene="deoB"
                     /locus_tag="ACHOS5_004094"
     CDS             11227..12450
                     /gene="deoB"
                     /locus_tag="ACHOS5_004094"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9778822.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            12507..13226
                     /gene="deoD"
                     /locus_tag="ACHOS5_004095"
     CDS             12507..13226
                     /gene="deoD"
                     /locus_tag="ACHOS5_004095"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9778823.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3219 a         3327 c         3538 g         3232 t
ORIGIN      
        1 attttggttg tctttaagaa ctgatatcgc tgtttgtaat aattctttgt tatccagcca
       61 tgactttttc tttatgtttc cttcaatgta atcaagcaat gttctggtat tgataggtct
      121 tccctgtttt gctacttcca cgacagcatc tcccaggata attctgactt caggaagctg
      181 cgcagggaac cactttaggg tgtcttttga tttcatgaag atattcctta aaatattatt
      241 ggttttcatt gcaatattgt atgtctgatt caggatatgt tgacttatac atcggttttg
      301 tccgggttat tggatatgcc aatccctaat tttattagag catgaccaaa aatgctgaat
      361 atgataagta gtgaagtgat tatcagtatg ctgttcatat agcctcgaat tagtatggtg
      421 ttatatataa tatagttgac aatttttatc ctgggtgttc ttaaagttcg tagatgaacc
      481 ctgtagtttc aggtatacag gaatgcttac agatagcggc aaaagtcagg aggttaatgg
      541 cgcaaactga aggggtagct gcgaactaac ttaggtagag actctacacg gattcggttt
      601 aaaagtatac atagataaca atttttatct gaagaagaaa aatattaagg tgatatggtc
      661 atatatactt taatgcggaa gaatgaatgt gatgagagtg ctgtatctga atggttgaaa
      721 aaccgcagac acgtcgtatg caagaacgtg ctgcggttgg ctggtgaact tccgatagtg
      781 cgagtattga atgatttcca gccgttaccg attttacgtg ttaattagtg aacaaaccac
      841 tcgtcagcag actcccaggt atctttcaga gtttcctgaa caaaagtttt agctgaatct
      901 ttatcggcgg tgcgcgtaac agaaaggcca tcgttgctgg tggcttttac gatcacctct
      961 acatcgtcat aacgcttact gatgcgtcga gttaattctt cctttaacgc atccacagca
     1021 ccgtttggca ttttagtcat tttctctttg gctatgcaga tctcaatacg cataaaaatc
     1081 cctctacact gtgtttgtat acagtgttat ttttaactgt atgaataaac agtgtcaata
     1141 gaacaaagat tgctgcatgt acaaatgttc tttggctaca aatacaccta ctgatcatgt
     1201 tctgctatgc tctgatacct ccaaaatgta caatcagcgg catgaatttt tctttgtaca
     1261 tatgattgta cataatcaaa gagacaacaa ttatgacaag ccttttggcc gcagaggtag
     1321 cgaaaagaag aacctttgca attatttctc acccggacgc cggtaagact accatcaccg
     1381 agaaggtgct gctgttcgga caggccattc agaccgccgg tacagtaaaa ggccgtggct
     1441 ctaaccagca cgctaagtcg gactggatgg agatggaaaa gcagcgtggg atctccatca
     1501 ctacgtctgt gatgcagttt ccgtatcacg actgcctggt taacttgctc gataccccgg
     1561 ggcacgaaga cttctcggaa gatacctatc gtaccctgac ggcggtggac tgctgcctga
     1621 tggttatcga cgccgcaaaa ggtgtagaag atcgtacccg taagctgatg gaagttaccc
     1681 gtctgcgcga tacgccgatc ctcaccttta tgaacaaact tgaccgtgat atccgcgacc
     1741 cgatggaact gctcgatgaa gttgagaacg agctgaaaat cggctgcgca ccgatcacct
     1801 ggccgattgg ctgcggcaag ctgtttaaag gcgtttacca cctttataaa gatgaaacct
     1861 atctctatca gagcggtaaa ggccacacca ttcaggaagt ccgcattgtt aaagggctga
     1921 ataacccgga tctcgacgct gcggttggtg aagatctggc acagcagctg cgtgacgaac
     1981 tggagctggt gaaaggcgcg tctaatgagt tcgacaaaga gctgttcctt gcgggcgaaa
     2041 tcaccccggt attctttggt actgcgctgg gtaacttcgg cgtcgatcat atgctggatg
     2101 gcctggtgga gtgggcgcct gcgccgatgc cgcgtcagac cgatacccgt accgtagaag
     2161 cgagcgaaga taaatttacc ggcttcgtat ttaaaattca ggccaacatg gacccgaaac
     2221 accgcgaccg cgtggcgttt atgcgcgtgg tatccggtaa atatgaaaaa ggcatgaagc
     2281 tgcgccaggt gcgcaccgcg aaagatgtgg tgatctccga cgcgctgacc tttatggcgg
     2341 gtgaccgttc gcacgttgaa gaagcgtatc ctggcgatat ccttggtctg cacaaccacg
     2401 gcaccattca gatcggcgac acctttaccc agggtgagat gatgaagttc accggtattc
     2461 cgaacttcgc gccagaactg ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac
     2521 agctgctcaa agggctggta cagctttccg aagagggcgc ggtgcaggtg ttccgtccga
     2581 tctccaacaa cgatttgatc gttggcgcgg ttggtgtgct gcagtttgat gtggtggtag
     2641 cgcgcctgaa gagcgaatac aacgttgaag cagtgtatga gtcagtcaac gttgccactg
     2701 cccgctgggt agaatgtgcg gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa
     2761 gccaactggc gcttgatggc ggcgataacc tcgcttacat cgctaccagc atggtcaacc
     2821 tgcgcctggc gcaggaacgt tatccggacg ttcagttcca ccagacccgc gagcattaat
     2881 tcttgcctcc agggcgcggt agccgctgcg ccctgtcaat ttcccttcct tattagccgc
     2941 ttacggaatg ttcttaaaac attcactttt gcttatgttt tagctgatat cccgagcggt
     3001 ttcaaaattg tgatctatat ttaacaaagt aatgacattt ctgacggcgt taaataccgt
     3061 tcaatgcgta gatatcagta tctaaagccg tctattgtca ttctaccgat attaataact
     3121 gattcagagg ctgtaatggt cgttattcat cacccatcgc ttttgtgatg gcgaccattg
     3181 acttctgtag agggtgaagt ctctccctat tcagcaatgc aacctcgtgt tgccaggctc
     3241 aaattacgag caaacataca ggaataaatc gatgactatg acaagactga agatttcgaa
     3301 aactctgctg gctgtaatgt tgacctctgc cgtcgcgaca ggctctgcct acgcagaaaa
     3361 caacgcgcag actaccaatg aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt
     3421 cggtaatttc atggatgaca gcgccatcac cgcgaaagtg aaggcggccc tggtggatca
     3481 tgacaacatc aagagcaccg atatctctgt caaaaccgat caaaaagtcg tgaccctgag
     3541 cggtttcgtt gaaagccagg ctcaggccga agaggcggtg aaagtggcga aaggtgttga
     3601 gggcgtgacc tctgtcagcg acaaactgca cgttcgcgac gctaaagaag gctctgtgaa
     3661 aggctacgcg ggtgacaccg ccactaccag tgaaatcaaa gccaaactgc tggcagatga
     3721 cattgtccct tcccgtcatg tgaaagttga aaccaccgat ggcgtggtcc agctctccgg
     3781 taccgtagat tctcaggcac agagcgaccg tgctgaaagt atcgccaaag cggtagatgg
     3841 tgtgaaaagc gttaaaaatg atctgaaaac taagtaattc gtcgtaattc gacctcccga
     3901 actttgtcgg gaggcgcgat gtgcaccaca ctaaaaatat cgcattgagt gattttcacg
     3961 ctcatatcaa gcggtgacat taactatggt aaaggagacg cttatgtttc gttggggcat
     4021 catatttctg gttatcgcgt taatcgccgc cgcacttggg tttggtggtc tggccggtac
     4081 tgctgcaggc gcagctaaaa ttgtctttgt cgtcgggatt attctgttcc tggtgagttt
     4141 gttcatgggc cgaaaacgac cctagatttc agaccattaa gaaaatttta tccaaagcca
     4201 gtccagcgga ctggcttttg cggttttagc gaataataaa ttgcgttact ttgtcattct
     4261 ttcacaacaa aaacaggaag gcagaggtgg ggcagcgaat acctgtaacg cttggtaata
     4321 ttgcgccgtt gtcgctaagg ccgttccagc ctggacggat tgctctggtg tgcgaaggcg
     4381 gcggacaacg tggaattttc acggctggcg tgctggatga gtttatgcgc gcgcagttta
     4441 atcctttcga tctttatctc ggcacatctg ccggggcgca gaacctctcg gcgtatatct
     4501 gcaatcagcc cggttacgcg cgcaaagtca tcatgcgtta caccacaaaa cgcgaatttt
     4561 tcgatccatt gcgctttgtc cgtggaggaa atcttatcga tctcgactgg ctggtggagg
     4621 ccaccgcaag ccagatgccg ttgcaaatgg acaccgcagc gcggttattt gacagcggca
     4681 aatcgtttta tatgtgcgcc tgtcgtcagg atgactatgc gccgaattac tttttgccaa
     4741 ctaaacaaaa ctggctggat gtgattcgcg cctccagtgc gatacctggc ttttatcgta
     4801 gcggagtgtc gctggaaggt attaactacc tggatggtgg gatcagtgat gcgatcccgg
     4861 ttaaagaggc ggcaaggcag ggagcaaaaa cgttggtcgt catccgcact gtgccgtcac
     4921 aaatgtacta cacgccacag tggttcaaac gcatggaacg ctggctgggt gacagtagcc
     4981 tgcagccgct ggtcaatctg gtgcagcatc atgaaaccag ctatcgtgag attcagcaat
     5041 ttattgagaa accaccgggc cagctgcgga tattcgaaat ttatccgccg aagccattgc
     5101 atagtatcgc gctcggcagt cggattccgg cgctgcgtga agactataaa cttgggcgtt
     5161 tatgtgggcg ttattttctg gcaacggtcg gtaaattatt gactgaaaaa gcgccgctga
     5221 gccgccatct ggtgccggtg gtgacgccgg agtcgatcgt tattccgcct gcgccagtcg
     5281 ccaacgatac gctggttgcc gaagtgagcg acgctccgca ggcgaacgac ccgacattta
     5341 acaatgagga tctggcttga tttgccgttt tatcgacacc cactgccatt ttgatttccc
     5401 gccgtttagt ggcgatgaag aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa
     5461 gatcattgtt ccggcgacag aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa
     5521 atatcaaccg ctgtatgccg cattgggctt gcatcctggt atgttggaaa aacatagcga
     5581 tgtgtctctt gatcagctac agcaggcgct ggaaaggcat ccggcgaagg tggtggcggt
     5641 gggggaaatc ggtctggatc tctttggcga cgatccgcaa tttgagaggc agcagtggtt
     5701 actcgacgaa caactgaaac tggcgaaacg ctacgatctg ccggtgatcc tgcattcacg
     5761 acgcacgcac gacaaactgg cgatgcatct taaacgccac gatttaccgt gcactggcgt
     5821 ggttcatggt ttttccggca gcctgcaaca ggccgaacgg tttgtgcaac tgggctacaa
     5881 aattggcgta ggcgggacta tcacctatcc acgcgccagt aaaacccgcg atgccatcgc
     5941 aaaattaccg ctggcatcgt tgttgctgga aaccgacgca ccagatatgc cgctcaatgg
     6001 ttttcagggg cagcctaatc gccctgaaca ggccgcccgt gtgtttgccg tgctttgcga
     6061 gttacgaccg gaaccggcgg atgagattgc cgaagtgttg cttaataaca cctatacgtt
     6121 gtttaacgtg ccgtaggccg gacaaggcgt ttacgccgca tccggccgtt ggcgcacaat
     6181 gcctgatgcg acgctgacgc gtcttatcat gcctacaagt ctgtgccgaa ccgtaggccg
     6241 gataaggcgt tcacgccgca tccggcagct ggcgcacaat gcctgatgcg acgcttgacg
     6301 cgtcttatca tgcctacagg tctgtgccga tccgtaggcc acatccggca tgtcacaaat
     6361 atagcgccgg aaatattaac cggctcaccc cgcgctcccg taacgcatca gccagttgtt
     6421 ccacatcctc cggcgtggcg ctcggccagc tttgcgcctc gccatacacg ccgtgggcat
     6481 gaaacgcgtt caggcgtacc ggaacatcgc caagtccctt gattaacgcc gccagttctt
     6541 cgatatgttg caaataatcc acctggccag ggattaccag caaacgcagt tccgccagct
     6601 taccgcgctc tgccagcaaa cagatgctgc gcttgatctg ctgattatcg cgtccggtta
     6661 attgctgatg acattcgctc ccccacgctt tgagatcgag cattgcgccg tcacacaccg
     6721 ggagcaattt ttcccagccg gtttcgctca acatgccgtt actgtccacg aggcaggtga
     6781 gatggcacag ttgcggatcg tttttgatgg cagtaaacag cgccaccaca aacggcaact
     6841 gggtcgtggc ttcgccgccg cttaccgtta tcccttcaat aaacagaacc gctttgcgga
     6901 catggttaag cacctcgtcc acgctcatgg attgcgccat tggcgtagca tgttgcggac
     6961 acatcttcag gcaggtatca cattgctcgc aggcagccgc gctccacgcc actttgccgt
     7021 cgacaatctg caacgcctga tgcggacact gcggcacgca ctctccacaa tcattacagc
     7081 gccccatcgt ccacggattg tgacagtttt tgcagcgcag attgcagccc tgcaaaaaca
     7141 gagccagacg actgcctggc ccgtcaacgc aggagaaggg gataatctta ctgactaaag
     7201 cgcatctgct gttcatggct tatcacgcgc ggctggcgtt ccagaatacg agtgttgcgt
     7261 gcggcttctt ctcccagcca ggtagtgttg gtgcgtgaac cttcggcgcg atatttttct
     7321 aaatccgaca aacgcaccat ataaccggta acgcgaacca gatcgttacc gctgacattg
     7381 gcggtaaatt cacgcattcc ggctttaaag gcaccgaggc aaagctgtac cagcgcctgc
     7441 ggattacgtt tgatggtttc gtcgagcgtc agaatgtcgc tgatgccgga ataataataa
     7501 gcatgatgcg gggcgacagt ttgcagatgg gtaatcggat ctggctcatc accatacggc
     7561 aaacgcgcgc ccggcgtggt gccgatatcg gaacttatcc ccgactgtgc gtgtaacatg
     7621 gcgcgttttt gccagccata tttcacgggg gtattggcga caaactccgc cagttgcgcg
     7681 ctaatgcgat aacccacttc atttgcggcg gcctctttac cgtagcgtgc ggcaatccct
     7741 tctttttcac acagcaagtt aaccgcttcc gccagcccat acatgccaaa catcggcaca
     7801 aaacgttcag ggttaatcag cccttctttc accaggaagc tattctcaaa gaagtgtgat
     7861 tgttgataga ggaattcaca ccgcgcatcg atgatggcga tctgctgttg gcagtagtgc
     7921 ggtagagtgc gcgtaaagaa gtcctccagc gattcactgc gctcggcaat ggctttcagg
     7981 tttaggcgta ccagcgtgct gccaccaccc gccagcggca gtgagttgta acagctcaca
     8041 atcccgtagc ccccctttgt gaaaatttta tcatgcaccg gaccgttggc gatgtgcggt
     8101 ttgctacatt cacagatgtt tttcgccact tccagcagca ggtcatcagg ggtgatatca
     8161 ggatcgtaga taaaggtcag gttcggtgaa acctgcttca gttctgcgtc tgcacgtaat
     8221 atcgcacggg taatgggcga atcagacggg ccgatattgg catgcataaa ggcgtctggc
     8281 agggttctgt cgaggtaacg ccagaaacgt tttattcgaa tatcgatctc gtcttgtgtt
     8341 agaattctaa catacggttg caacaacgca tccagttgcc ccaggtagac cggcatcgat
     8401 gtgaccgacg gtacatggtg gtaaagaatg gtcagcagag agagtgcgtc atcaaggtct
     8461 ttcgcgcctt ccagctccag ccattcggaa ccgtgcgcca gaaaacgggc gtaatcgggt
     8521 aagacatagc gcggtttgta cggcgcatga ccttcaaaca tatcgcagat taccccctca
     8581 tccagcgcgc ggcgggcttc ggcaggcaac gcggggtaag gcagattgtt ttctgcttcc
     8641 agtgccagaa aatggcgctt ctgctccggg ctaagtaccg ggctggtgac aatttgctgg
     8701 caacgttgtt gcagtgcatt ttcatgagaa gtgggcatct tcttttcctt ttatgccgaa
     8761 ggtgatgcgc cattgtaaga agtttcgtga tgttcacttt gatcctgatg cgtttgccac
     8821 cactgacgca ttcatttgaa agtgaattat ttgaaccaga tcgcattaca gtgatgcaaa
     8881 cttgtaagta gatttcctta attgtgatgt acatcgaagt gtgttgcgga gtagatgtta
     8941 gaatactaac aaactcgcaa ggtgaatttt attggcgaca agccaggaga atgaaatgac
     9001 tgatctgaaa gcaagcagcc tgcgtgcact gaaattgatg gacctgacca ccctgaatga
     9061 cgacgacacc gatgagaaag tgatcgccct gtgtcatcag gccaaaactc cggtcggcaa
     9121 taccgccgct atctgtatct atcctcgctt tatcccgatt gctcgcaaaa ctctgaaaga
     9181 gcagggcacc ccggaaatcc gtattgctac ggtaaccaac ttcccacacg gtaacgacga
     9241 catcgaaatc gcgctggcag aaacccgtgc ggcaatcgcc tacggtgccg atgaagttga
     9301 cgtggtgttc ccgtaccgcg cgctgatggc gggtaacgag caggttggtt ttgacctggt
     9361 gaaagcctgt aaagaggctt gcgctgcggc gaacgtactg ctgaaagtga ttattgaaac
     9421 cggcgaactg aaagacgaag cgctgatccg taaagcgtct gaaatctcca tcaaagcggg
     9481 tgctgacttc atcaaaacct ctaccggtaa agtggctgtg aatgcgacgc cggaaagcgc
     9541 gcgcatcatg atggaagtga tccgcgatat gggcgtagaa aaaaccgttg gtttcaaacc
     9601 ggcgggcggc gtgcgtactg cggaagatgc gcagaaatat ctcgccattg cagatgaact
     9661 gttcggtgct gactgggctg atgcgcgtca ctaccgcttt ggtgcttcca gcttgctggc
     9721 aagcctgctg aaagctctgg gccacggcga cggtaagagc gccagcagct actaaggctt
     9781 gatttgccgg atgatgcagc gatgctcatc cggttccccg attacctcgt ctcttttcca
     9841 cgggaggtta ccttgtttct cgcacaagaa attattcgta aaaaacgtga tggtcatgcg
     9901 ctgagtgatg aagaaattcg tttctttatc aacggcattc gcgacaacac tatctccgaa
     9961 gggcagattg ccgccctcgc gatgaccatt ttcttccacg atatgacaat gcctgagcgt
    10021 gtctcgctga ccatggcgat gcgagattca ggaaccgttc tcgactggaa aagcctgcat
    10081 ctgaatggcc cgattgttga taaacactcg accggcggtg tcggcgatgt gacttcgctg
    10141 atgttggggc cgatggtcgc tgcctgtggc ggctatattc cgatgatctc cggtcgcggc
    10201 ctcggtcata ctggcggtac gctcgacaaa ctggaatcca tccctggctt cgacattttc
    10261 ccggatgaca accgtttccg cgaaattatt aaagacgtcg gcgtggcgat tatcggccag
    10321 accagctcat tggctccggc ggataaacgt ttctacgcga cccgcgatat taccgcaacc
    10381 gtggattcaa tcccgctgat caccgcctct attctggcga agaaactggc ggaaggtctg
    10441 gacgcgctgg tgatggacgt gaaagtgggt agcggcgcgt ttatgccgac ctacgaactc
    10501 tctgaagccc ttgccgaagc gattgttggc gtggctaacg gcgctggcgt gcgcaccacc
    10561 gcgctgctca ccgatatgaa tcaggtactg gcctccagtg caggtaacgc ggttgaagtt
    10621 cgtgaagcgg tgcagttcct gacgggtgaa tatcgtaacc cgcgtctgtt tgatgtcacg
    10681 atggcactgt gcgtggagat gctgatctcc ggcaaactgg cgaaagatga cgccgaagcg
    10741 cgcgcgaaat tacaggcggt gctggacaac ggtaaagcgg cagaagtctt tggtcgtatg
    10801 gtagcggcac aaaaaggccc gaccgacttc gttgagaact acgcgaagta tctgccgaca
    10861 gcgatgctga cgaaagcagt ctatgctgat accgaagggt ttgtcagtga aatggatacc
    10921 cgcgcgctgg ggatggcagt ggttgcaatg ggcggcggac gccgtcaggc atctgacacc
    10981 atcgattaca gcgtcggctt tactgatatg gcgcgtctgg gcgaccaggt agacggtcag
    11041 cgtccgctgg cagttatcca cgcgaaagac gaaaacagct ggcaggaagc ggcgaaagcg
    11101 gtgaaagcgg caattaaact tgccgataaa gcaccggaaa gcacaccaac tgtctatcgc
    11161 cgtatcagcg aataacggta tactgatctg atcatttaaa tttgaagcac tgagtacgga
    11221 gaacatatga aacgtgcatt tattatggtg ctggactcat tcggcatcgg cgctacagaa
    11281 gatgcagaac gctttggtga cgtcggggct gacaccctgg gtcatatcgc agaagcttgt
    11341 gccaaaggcg aagctgataa cggtcgtaaa ggcccgctca atctgccaaa tctgacccgt
    11401 ctggggctgg cgaaagcaca cgaaggttct accggtttca ttccggcggg aatggacggc
    11461 aacgctgaag ttatcggcgc gtacgcatgg gcgcacgaaa tgtcatccgg taaagatacc
    11521 ccgtctggtc actgggaaat cgccggtgtc ccggttctgt ttgagtgggg atacttctcc
    11581 gatcacgaaa acagcttccc gcaagagctg ctggataaac tggttgaacg cgctaatctg
    11641 ccgggttacc tcggtaactg ccactcttcc ggtacggtca ttctggatca gctgggcgaa
    11701 gagcacatga aaaccggcaa gccgattttc tatacctccg ctgactccgt gttccagatt
    11761 gcctgccatg aagaaacttt cggtctggac aaactctacg aactgtgcga aatcgctcgt
    11821 gaagagctga ccaacggcgg ctacaacatc ggtcgtgtta tcgctcgtcc gtttatcggc
    11881 gacaaagctg gtaacttcca gcgtactggt aaccgtcacg acctggctgt tgagccgcca
    11941 gcaccgaccg tgctgcagaa actggttgat gaaaaacacg gccaggtggt ttcggtcggt
    12001 aaaattgcag acatctacgc caactgcggt atcaccaaaa aagtgaaagc gaccggcctg
    12061 gacgcgctgt ttgacgccac catcaaagag atgaaagaag cgggtgataa caccatcgtc
    12121 ttcaccaact tcgttgactt cgactcttcc tggggccacc gtcgcgacgt cgccggttat
    12181 gctgcgggtc tggaactgtt cgaccgccgt ctgccggagc tgatgtcttt gctgcgcgat
    12241 gacgatattc tgattctcac tgctgaccac ggttgtgacc cgacctggac cggtactgac
    12301 cacacgcgtg aacacattcc ggtactggtt tacggcccga aagtgaaacc aggttcgctg
    12361 ggtcatcgtg aaaccttcgc ggatatcggc cagactctgg caaaatattt tggtacttct
    12421 gatatggaat atggcaaagc catgttctga tggatttggg cggagcagtg actccgcctt
    12481 taatatgtca caaaaaggat aaaacgatgg ctactccaca cattaatgca gaaatgggcg
    12541 attttgctga cgtagttttg atgccaggcg acccgctgcg tgcgaagtat attgctgaaa
    12601 ctttccttga agatgcccgt gaagtgaaca acgttcgcgg tatgctgggc ttcactggta
    12661 cttacaaagg ccgcaaaatt tccgtaatgg gccacggtat gggtatcccg tcctgctcca
    12721 tctacaccaa agaactgatc actgatttcg gcgtgaagaa aattatccgc gtgggttcct
    12781 gtggcgctgt tctgccgcac gtaaaactgc gcgacgtcgt tatcggtatg ggtgcctgca
    12841 ccgattccaa agttaaccgc atccgtttta aagaccatga ctttgccgct atcgctgact
    12901 tcgacatggt gcgtaacgcg gtagacgcgg caaaagcact gggcgttgat gctcgcgttg
    12961 gtaacctgtt ctccgctgac ctgttctact ctccggacgg cgaaatgttc gacgtgatgg
    13021 aaaaatacgg catcctcggc gtggaaatgg aagcggctgg tatctacggt gtcgctgcag
    13081 aatttggcgc gaaagccctg accatctgca ccgtgtctga ccacatccgc actcacgagc
    13141 agaccactgc cgctgagcgt cagaccacct tcaacgacat gatcaaaatc gcgctggaat
    13201 ccgttctgct gggcgataaa gagtaattgt gttttgctga aaagcgattg tcgtgtgaag
    13261 ccggagtggg agactgctcc ggctttttag tatttattca tcaatagcaa ctgatt
//
LOCUS       DAZKHK010000132        13236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKHK010000132 DAZKHK010000000
VERSION     DAZKHK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.133"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            149..1648
                     /gene="pitB"
                     /locus_tag="ACHOS5_004096"
     CDS             149..1648
                     /gene="pitB"
                     /locus_tag="ACHOS5_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP9778824.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLNGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1697..2389)
                     /gene="yghT"
                     /locus_tag="ACHOS5_004097"
     CDS             complement(1697..2389)
                     /gene="yghT"
                     /locus_tag="ACHOS5_004097"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9778825.1"
                     /translation="MQSIIPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            2564..3277
                     /gene="yghS"
                     /locus_tag="ACHOS5_004098"
     CDS             2564..3277
                     /gene="yghS"
                     /locus_tag="ACHOS5_004098"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9778826.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELASRMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLLAIHAALS"
     gene            3309..4067
                     /locus_tag="ACHOS5_004099"
     CDS             3309..4067
                     /locus_tag="ACHOS5_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778827.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGTGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4113..5462
                     /locus_tag="ACHOS5_004100"
     CDS             4113..5462
                     /locus_tag="ACHOS5_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP9778828.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTIRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNSTGEKS"
     gene            5462..6298
                     /locus_tag="ACHOS5_004101"
     CDS             5462..6298
                     /locus_tag="ACHOS5_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778829.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGHFQDGFRNGKKNIVSFYGNLLRVYLSSPVWWLVNYSLRHSQ
                     LETVNGFIAYRQKQMYILKEIIAKPQLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALLISRFLAAINGLMGDKHDDMVADDMDNRRSYDAPVALDSDIRQRLELLISRFPLYP
                     EQ"
     gene            6298..6858
                     /locus_tag="ACHOS5_004102"
     CDS             6298..6858
                     /locus_tag="ACHOS5_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9778830.1"
                     /translation="MPSRWIVALIIALLAPGAAAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLTGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFSQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWQLPEQSSTILVLNKAGRI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6889..7959
                     /gene="lptF"
                     /locus_tag="ACHOS5_004103"
     CDS             6889..7959
                     /gene="lptF"
                     /locus_tag="ACHOS5_004103"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9778831.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYASVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7956..9035
                     /gene="lptG"
                     /locus_tag="ACHOS5_004104"
     CDS             7956..9035
                     /gene="lptG"
                     /locus_tag="ACHOS5_004104"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9778832.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWGASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSTFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWHDRQH"
     gene            complement(9070..10242)
                     /locus_tag="ACHOS5_004105"
     CDS             complement(9070..10242)
                     /locus_tag="ACHOS5_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9778833.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10242..10490)
                     /locus_tag="ACHOS5_004106"
     CDS             complement(10242..10490)
                     /locus_tag="ACHOS5_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9778834.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENN"
     gene            complement(10522..11436)
                     /locus_tag="ACHOS5_004107"
     CDS             complement(10522..11436)
                     /locus_tag="ACHOS5_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9778835.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPQPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11433..13157)
                     /locus_tag="ACHOS5_004108"
     CDS             complement(11433..13157)
                     /locus_tag="ACHOS5_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP9778836.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLRGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVVLQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPSDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
BASE COUNT         3194 a         3260 c         3426 g         3356 t
ORIGIN      
        1 tctaaaggtt ttgaatgaag gcttattgtc tgatacacaa gaaataacac gctttttatc
       61 gttaaaaaat gatatttcac tttgcccacg ccgtgaaagt cactgataat gcctccgttc
      121 gtaaattcaa aatggcgtaa tctaatatat gctaaattta tttgttggcc ttgatatata
      181 tacggggctt ttgttattac ttgctctggc atttgtgttg ttctatgaag caatcaatgg
      241 ttttcatgac acggcgaatg cggtggcaac cgttatttat acccgtgcca tgcaaccaca
      301 gcttgccgtg gtcatggcgg cattttttaa cttttttggc gtattgcttg gcgggcttag
      361 cgttgcctat gccatcgtcc atatgttgcc aaccgatttg ttgctgaata tggggtcaac
      421 tcacggtctg gcgatggtct tttccatgct gctggcggcg attatctgga acctgggaac
      481 gtggttcttt ggtttaccgg cctccagttc gcacactttg attggtgcga ttatcggcat
      541 tggtttaacc aacgcgctgt taaatggttc gtcggtgatg gatgcgttaa acctgcgcga
      601 agtcaccaag attttctctt cactcattgt ctctcccatc gtggggctgg tgattgcggg
      661 ggggctaatt tttctgctac gccgtttctg gagcgggacg aaaaagcgtg accgtattca
      721 ccgcattccg gaagatcgca aaaagaaaaa aggcaaacgt aagccgccat tctggacgcg
      781 tattgccctc attgtttccg ctgcgggcgt ggcgttttcg cacggcgcga acgacgggca
      841 gaaaggaatt ggtctggtga tgctggtgct ggtggggatt gctcccgccg ggtttgtcgt
      901 caatatgaat gcgtccggct atgaaattac ccgtacccgc gatgccgtca ccaacttcga
      961 acactacctg caacagcatc ctgaactgcc gcagaagttg attacgatgg aacctccatt
     1021 gcctgcgaca tcgactgatg gcacgcaagt aacagagttt cactgtcatc cggcaaatac
     1081 ctttgatgcg attgcgcgcg ttaaaacgat gctgccaggc aatatggaaa gttacgagcc
     1141 gttaagcgtg agtcagcgca gccagctgcg ccgcattatg ctgtgcatct ctgatacctc
     1201 cgcgaagctg gcgaaactgc caggcgtcag taaagaagac cagaacctgc tgaaaaaact
     1261 gcgcagcgat atgttaagca ccattgagta cgcaccggta tggatcatca tggcggtagc
     1321 actggcgctc ggcatcggca ccatgattgg ctggcgtcgt gtagcgatga ccatcggtga
     1381 gaagattggt aagcgcggca tgacgtatgc gcaaggcatg gcggcacaaa tgacggcggc
     1441 agtgtctatc ggccttgcca gttatattgg gatgcccgtc tccacaacac acgtactctc
     1501 gtctgcagta gcagggacga tggtggtgga cggcggtggg ttacagcgta aaacggtaac
     1561 cagcatcctg atggcgtggg tgtttacttt accggcggcg atttttcttt ctggtgggtt
     1621 gtactggata gcattgcagt tgatttaacc ttcctgaaaa tgcccggtcc tggcgatcgg
     1681 gcatttccat ttttcactag tgataaccac gcgcggtcat aaaatccgta atcgcttttt
     1741 ctgcatcaac cagcaccttt tccagtggct gattggcgtc gatatcaacc agttgtgcac
     1801 caccaaaggt taactgtggc gttatggcaa tcttcctcgc cagcgattcc cgtttatggt
     1861 cgggtttacg cgcgcaggca acttcaaggt caacattaag tttgatgacc agatcgggct
     1921 tgtggctcgc catccagtga aatgctttac gttcctgact tgccagccat gagacaaaac
     1981 gaccaccttc aacgttaggc ggaaacaccg taccatcata agcgccagga atctggtcct
     2041 gcggataacg gtcggttaaa acaattaacc cgcgacgacg acaggcaagc atatgacgaa
     2101 agcgcagtaa gcggcgggcg acaaacgctg taattaccag cgccggaact ggtccaggca
     2161 atttttttgc tgtttttacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
     2221 acggtaattt tgtcactgca cgaccaacat ttccggcctg ttttcctaaa tgaacccttt
     2281 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
     2341 aaccatcgct accaataacg gcaattaatg gaggtattat tgactgcatg aatatatcct
     2401 taaatttaaa tatccatcca aaatatttat ttggttaata tgtttttgat gaaagcgtaa
     2461 ttcaggtcaa tgtcacaatt aaccattgtc acaataaggt tgaacggata ttctgagcac
     2521 ggaccacgta atattcaata cattatttgc tgccgggttt ttcgtgaatc aacgtaatat
     2581 gtcaataatt aattccacgc cagtgcgtgt tattgccatt gtaggatgtg atggttcagg
     2641 taaatcgacc ctcacggcaa gcctggtaaa tgaactggca tcaagaatgc caacagaaca
     2701 catttatctc gggcaatcgt ccgggcgaat tggcgaatgg atttcacagc tacctgttat
     2761 tggcgcacct tttgggcgtt atttgcgaag taaagcggca catgtgcacg aaaagccctc
     2821 aacaccgcct ggcaatatta ctgcgctggt tatctatctg ctttcctgct ggcgggcgta
     2881 caagtttcgc aaaatgttgt gtaaaaacca gcaaggctat ctgctcatca ccgaccgcta
     2941 cccgcaggtt gaagtgccgg gtttccgctt tgatggcccg caattggcaa aaaccacggg
     3001 cggtaacggt tggataaaaa tgttaaggca gcgtgagctg aagctgtacc aatggatggc
     3061 ttcttatttg cctgtattgt tgattcgtct tggcattgat gaacaaaccg cgtttgcgcg
     3121 taaacctgac caccaactgg cagcgttaca ggaaaaaatc gccgttacgc cgcaactgac
     3181 attcaatggc gcaaagatcc ttgaactgga tggtcggcaa ccagccgatg aaattctgca
     3241 agcgtcacta ctcgcaattc acgccgcgct ttcctgatca ccaaaatgga taggggatga
     3301 ttaaacgaat ggatgcacta caaacacaaa ctgttaagag tacaaccgcg ccgcagccca
     3361 actacattcc ggggctgatt gcggtggtcg ggtgtgatgg aaccggtaaa tccacactga
     3421 ccaatgacct ggtgaaatcg ctgcaacaac actggcaaac cgagcggcgc tatctggggc
     3481 tgctctccgg cgaagacggc gacaaaatca aacgattgcc gttggttggt gtctggctcg
     3541 aacggcgact ggcggccaaa tcctcgaaaa cccaaagcat gaaaaccaaa tctccggcgc
     3601 tatgggcggc ggtgattatg tactgcttct cgctgcgaag aatggcgaat ctgcgcaagg
     3661 ttcagcgact ggcgcaaagt ggcgttctgg tggtcagcga tcgcttcccg caggctgaaa
     3721 tttcgggctt ttattatgac ggaccgggga ttggcgtcga acgtgcgacc gggaaaatca
     3781 gcatgtttct ggcgcagcgt gaacggcgtt tataccaaaa aatggcgcaa taccgtcctg
     3841 aattaatcat tcgcttgggg attgatattg atactgccat ctcccgcaag cctgaccatg
     3901 actatgccga gctgcaggac aaaatcggcg tcatgtcgac gattggctat aacggcacaa
     3961 aaattcttga gattgattcc agagcgcctt acagcgaggt gctggaacag gcacagaagg
     4021 cggtttctct ggttgccatc gtttctgacc gccgaagttt aacttaaggc tggcgggata
     4081 ttttttacta tcaacgataa ggtcaggctg aattggcggg ttttaacatc aaacattggt
     4141 ttgcagatgg cgcatttcgc accattattc gcaatagcgc ctggttaggc tccagtaatg
     4201 ttgtgagcgc tttgctgggg ctgttggcgc tctcgtgtgc cggtaaaggg atgacgcccg
     4261 ccatgtttgg cgtactggta attgtgcaat cgtacgccaa atcgatcagc gattttatta
     4321 agtttcagac atggcaactg gtggttcagt acggaacgcc ggccttgacc aacaataatc
     4381 cgcagcaatt tcgcaatgtt gtctcatttt ccttctcgct ggatatcgtc agcggcgcgg
     4441 tggcgattgt cggcggcatt gccttactgc cattcctttc ccattcatta ggtctggatg
     4501 accaaagctt ttggctggcg gcgctctatt gcacgctcat tccttcaatg gcttcctcca
     4561 cgccgaccgg cattctgcgt gcggtagatc gctttgattt gattgctgta cagcaggcga
     4621 cgaaaccttt tctgcgcgca gcggggagcg tcgtagcctg gtattttgac tttggttttg
     4681 cgggttttgt tattgcctgg tacgtgtcga atctggttgg cggcaccatg tactggtggt
     4741 ttgccgcgcg cgaattacgc cgcagaaata tccataacgc cttcaaattg aatctgtttg
     4801 agtctgcccg acacattaaa ggcgcgtgga gttttgtctg gtcaaccaac attgcccact
     4861 ccatctggtc ggcgcgtaac tcgtgcagca cagtgttggt ggggatcgtg ttaggacccg
     4921 ctgctgccgg gttatttaaa atcgccatga cattcttcga tgccgccgga acgccagcgg
     4981 gtttactggg caaaagtttt tatccagagg tgatgcgttt agatccacgc accatcagac
     5041 cgtggttgtt aggcgtgaag tccggtttac tggcgggcgg aatcggtatt ctggtggcgc
     5101 tcgcggtgtt gatcgtcggc aagccgctca tttcgctggt gtttggcgtt aagtatctcg
     5161 aagcgtatga cctgattcag gtgatgctgg gcgcaattgt gatttcgatg ctgggcttcc
     5221 cacaggagtc attgctattg atggcgggaa aacagcgcgc ttttctcgtg gcgcaaacca
     5281 tcgcctcaat cggctacatc gtattgctgt tcatgttctg tcatctgttt ggcgtgctgg
     5341 gggctgcatt tgcttacttc ggtgggcaat gtctggatgt ggtgctctcg ctaattccga
     5401 ccttaaaggc attttttcag cgccattcct tgctttataa ctcaaccgga gagaaatcct
     5461 gatgatgaga aaatatttcc cgcttgaggc gagcgagcgt ctatttgttg cgattgaaga
     5521 agatgatgtt gttgatgcac aggtcagttt gccgccgact atcgcactaa gttgtaccac
     5581 cgaaatcatc catgataatt acgcgttatg cctgaaattc tggctggatg gtgttaatcg
     5641 tcaggagtta ctgcgtttaa tccgcaaaca ggcgaaaggc gatgagctga cgacagacga
     5701 gcgcaaacag tttaaataca tgcgagcgcg ctacaagcat ctgcgttttg cgcaacggct
     5761 gtatttaaag aagcatcagg ccggattttt attcggcaaa acgaccgttt ttcttgggca
     5821 ttttcaggat ggctttcgta acggtaagaa aaatattgtg tcgttttacg gtaacctgtt
     5881 acgtgtttat ttgagctccc ctgtctggtg gctggtgaac tattcattgc gccatagcca
     5941 gttagaaacc gtgaacggtt ttatcgccta ccgccagaag caaatgtaca tcctgaaaga
     6001 gattattgct aaaccgcaac taactggcag ggaatttcat gatgtgcgaa aaatcatcag
     6061 ccagcaggtt tcttattacg acaccttaag atcgcttgat cctgaaaata aagaggcgtt
     6121 actgatttcc cgttttctcg ccgccattaa tggtctgatg ggggataagc acgacgacat
     6181 ggtggcggat gatatggata accgtcggtc atatgatgca ccggtggcgc tggatagcga
     6241 tattcgccag cgtttagaac tgcttatttc gcgtttccca ctatatccgg agcaataatg
     6301 ccatctcgtt ggattgtcgc tctgatcatc gcactcttag cgccaggcgc ggcggcacac
     6361 aatttcgtca ctggcaaaac ggttactcct gtttatatcc aggaaggcgg cgagctgctt
     6421 ctgaatagcg acgacgaaat tcactaccaa aagtggaaca gcacacaatt gacgggaaaa
     6481 gtccgcatta ttcagtatat cgccggacgt aagtctgcga agaaaaagaa ctcactgtta
     6541 attaaagcgg ttgaagcggc aaacttttcg caggatcgtt ttcagcccac cactattgtg
     6601 aacaccgatg atgccatctt tggtactggc tattttgtcg ttggcaaaat cgaaaaaaat
     6661 aaacgccgct atccgtgggc gcaattcgtt attgacggca atggtctggg acgcgttgcc
     6721 tggcaattgc cggagcagag ttcaaccatt ctggtattga ataaagccgg gcgaattcag
     6781 tgggcaaagg acggcagcct gacaccggaa gaggtagatc atgtcattgc gctggcgcag
     6841 aaactgatca atgaataaaa aattcaaagg attgagggcg ttagcgagat gaagctggtt
     6901 gaacactaca tcatgcgtgg tacgcgccgt ctggtgctga ttatcgtcgg ttttctgatc
     6961 tttattttcg ccagctactc cgcacagcgt tatctgaccg aagcggcaaa tgggacatta
     7021 gctcttgatg ttgtgctgga tatcgttttc tacaaagtgc tgattgcact ggagatgctg
     7081 ttacctgttg gactgtatgc gtcagttggc gtgacgctag ggcaaatgta taccgactcg
     7141 gaaattaccg ctatctccgc ggcggggggc agtccgggac gtttgtacaa agccgtcctt
     7201 tatctggcga taccgctaag tatttttgtc acccttctgt cgatgtatgg tcgtccctgg
     7261 gcttatgcgc agatttatca actggagcaa caatcgcagt cggagctgga tgttcgtcag
     7321 ttgcgggcaa agaaatttaa cactaacgat aacggacgaa tgatcctttc gcagacggtt
     7381 gatcaggata ataatcgcct gactgacgcg ctgatttata cttctactgc caatcgaacc
     7441 cgcattttcc gcgcccgttc ggttgatgtg gttgacccat cacccgagaa accgaccgtt
     7501 atgttgcata acggtaccgc ctatcttctc gatcatcagg ggcgtgacga caacgaacag
     7561 atctaccgta atctgcaatt gcatctcaat ccgctggatc aaagccctaa cgtcaaacgc
     7621 aaagcaaaat cggtcacgga gctggcacgc tccgtctttc cagccgatca tgccgaactg
     7681 caatggcgac aaagccgtgg cctgacagca ttgttgatgg cgctgctggc catttcatta
     7741 agtcgggtaa aaccgcggca ggggcgattt tcaacgctac tgccactgac gttgctgttt
     7801 gttgccattt tttatggcgg cgacgtctgc cgtacgctgg tggctaacgg cgcgattccc
     7861 ctcattcctg gtttgtggtt agtacccgga ctcatgctaa tgggcctgct gatgctggtc
     7921 gcacgcgact tctctttgct gcagaaattt tcccgatgaa tgttttcagt cgctatttaa
     7981 tccgtcacct ctttctcggt tttgccgccg ccgcagggct attactgccg ctttttacca
     8041 ccttcaacct gattaacgaa ctggatgatg tcagcccggg cggttatcgc tggacccagg
     8101 cggtgctggt ggtgctgatg accttaccgc gcacactggt cgaactttcg ccgtttatcg
     8161 ccttattggg aggtattgtc ggtctcgggc agctatcgaa aaacagtgag cttaccgcca
     8221 ttcgcagcac ggggttttct atcttccgta ttgcactggt ggcgctggtt gccgggatac
     8281 tgtggactgt ttcgttaggc gcgatagatg agtggggggc gtcgccattg cagcaacagg
     8341 cgctgcaaat caaatcgacc gccaccgcgt tgggggagga cgatgacatt accggcaata
     8401 tgctttgggc caggcgcggt aatgaatttg tgacggtgaa atcgctgaac gagcagggcc
     8461 agccggtggg cgtggagatt tttcattacc gcgatgatct ctcgctcgaa tcctacattt
     8521 atgcgcgcag tgccaccatt gaagatgaca aaacgtggat cctgcatggt gtgaatcata
     8581 aaaaatggct gaatggcaaa gaaacgctgg aaacatctga taatcttgcc tggcaatcga
     8641 ccttcaccag tatgaatctt gaagagttat cgatgccggg gaatactttt tctgtccgtc
     8701 agcttaatca ttacatccat tatttgcagg aaaccgggca acccagcagc gaataccgcc
     8761 ttgcactgtg ggaaaaactg gggcaaccca tcttgactct ggcgatgatt ttgctggctg
     8821 tgccgtttac ctttagtgcc ccgcgctcgc cagggatggg tagccgtctc gctgtaggcg
     8881 tcatcgttgg tttactcacc tggatcagct atcaaatcat ggtcaatctg ggattgttat
     8941 ttgcgttgag cgcacctgtt accgcgctcg gtttaccggt agcgtttgtg ctggtggcgt
     9001 tgagcctggt gtattggcat gacagacaac attaaacccg ccgtggcgca acaccacggc
     9061 gggttcacat tactgcttca gagtagcaaa tgcctgaatg atggcatcga tttgctcacg
     9121 actgtgggca gcgtttacgc tacagcgcag cagcgtgatg ccagcaggcg cggcaggtgg
     9181 caggataaga ttcacataaa cgcccagcga aataagctcg cgccagatgc gtaaaccttc
     9241 ctctttcgaa ccgattatta ctggcactac cgggctaata tgtgaactta actcgtaacc
     9301 cagtttttgc agaccgtcat acagatggtt ggcgttatcc atcagctttt ggcgcagctc
     9361 tggttgtgag gcaatggttc ttaacgacga gcgcacggtc gcaatgcagg acggagaagg
     9421 agaggcggta aaaatatacg ggcggctgcc gtagcgcagc acttccatcg cttctgaacc
     9481 cacggcaaaa ccaccaatgg aagccaaact tttactgaag gtgccgacga taatatccac
     9541 gtcgtcttca acgccaacgg cttcaaccag tccgcgcccc gtagcgccta acacgccaaa
     9601 cgaatgtgct tcatcgacaa tcaaataacc accgagacga cgtttgatat caacaatttc
     9661 tgccagcggc gcaacgtcac cgagcatact gtagatgcct tcaacgataa taatcgcctc
     9721 tttcgcccgt tcaccgaggc ggaccatgcg ccgttccaga tcgttgacgt cattatggcg
     9781 gaaacgaata atctccgcgc cgccgagaga acaggcgtca taaatactgg cgtggctgtc
     9841 ggcatccagt aacaccacgg cgttatggtc ggcaagtgcg ctgatgacac ctaaattagc
     9901 ggtataaccg gttgaaaaaa cgatagcggt agggcgattg aaaaactcgg caatctcttt
     9961 ctctaacgcc agatgcggcg cataactgcc gtttgccata cgagaaccgg tggtgccggt
    10021 gccttgggcc gctaacgctg cctgaccttc gcggatagca tcatgattaa aggttaaacc
    10081 gagataatta ttggtcccgg ccagaatcac ttttttattg ccgatccggc cttcggtggc
    10141 ggaatacact tcatcaatac aggtgccaaa tggtgttaac ccgttgtcac taaattgcag
    10201 gcgctcgcca gcaaggcgcg catatttatc gtatagcccc attagttatt ctccagccat
    10261 ggaagtagag tttccattaa ttgcgcaggg gttttgacat ccagcagaat attaatagga
    10321 atggaaatat cgaatctgtc ttccagcatc attaagagat ccatcacttt gatcgactcc
    10381 aggccaagat cgttgacgag atcgctgtcg ggcttaattt ccacaccgtt ttcgaccaaa
    10441 tcctgtaaac aggaaaggat gtaatccatt actatttcac gatttaccat agaaacgtta
    10501 acacaccttt aaacaagaca atcaaaatag cccgttgcgt aatgcgtgtt ccaggctaat
    10561 cccgggaaac caattaatat cttcagaaat acggttatta cttgccgacc agtcggcatg
    10621 ggttaattca cggattttcg aacgagtcag catcggttct ttacccgcca gacggctcaa
    10681 cgcggtactg atatccgcaa ggcaggtgag tagcggctga ggaataccga ccatccgcac
    10741 ggaaccacag cggacatcgg caacaagctg ttggacacgt tgccagtcat agcctccggc
    10801 gacgccatcg cataattcat aggtttgcgt ctgtacagtt tcggcgctta accactgacc
    10861 cactgcttgc gcaaaatcgg tgacgtgaag aaaagagagc tgtgtctctg gcgtgccaag
    10921 tcgtggcagc agaccgcgca gcatccagtc aaacagcggt tttaactctt tatcgccggg
    10981 accatacacg gctgtcgggc gaaaaacgcc caacgtaatt tcgtcagcca ttgcagtcag
    11041 ccgttgttcg gcgacgtgtt tggaatttgc gtaccaggag agttcgggat ggcgcgccgc
    11101 cagcgaagag ataaacagaa aacgttggca aaagccgctc tcttttgccg cttgcatcag
    11161 gcgcaggctg ccgtcaacgt tgcagcgggt aaaaatctct tctttgtgcc cgcgcacttg
    11221 cccggcgcaa tggaccaccg cgctggctcc ggcaaccagt tcgctaagcg aatgtgtatc
    11281 ttccagcgaa ccgcgcaccc aagtgagatt atcattgacg tgagcgcggg cagtacgcgt
    11341 caatgcgcga acgtgaaagc cgcgggcgag caggttatca ataatatatt taccgataaa
    11401 cccggtagcg cccgtcaccg cgacagtttg attcatgcca gggattcctg cacatgaaga
    11461 ctggcagcat aagccttctg ataacgtttt ttcgcttccg cacgggcagg cttgccggag
    11521 gatgttcggg gaatactgtg gggcggcaac agatcgatat ccgcggtaac gccaaattcg
    11581 ctttggatcc gagctgccag cgcgtggata agctgcccgc gacgttcttc gtcgctgatc
    11641 cgacactgga tctgcaaaat gattttttcc tgggcggtaa caaaagcaat cgcatcgcca
    11701 gaatgaattt ccggctcctg ttccgcaatg tattcaatat cctgcggcca gatattacgg
    11761 ccacgaataa taatcagatc tttaatgcgt ccggtgacat acagataacc gtccagcaga
    11821 taaccaaggt cgccggtgtc taaccagccc gtcgcggcaa tctcgtcttg cgaaacctgg
    11881 tcgccaaagt acccgctcat caggctgggg ccggagatgc aaatatggcc taccacacgt
    11941 tccgcgacag gtatatctgc ttcattgcgg atttcaatgc catgttccgg caacgcttta
    12001 ccgcagttga cgaaagtcga tacggcgcgt gtttcagcac ccggcgcgac ggctttaccc
    12061 tggtattcga ggatgtcgcg atccacttcg ttaaccacaa ccccgcaggc ttcatcagag
    12121 aagctgacag ccagcgcatt ttctgccagt ccgtagcacg gcatgaaagt tttatcgtca
    12181 aagttaacct gacggaaaca ttcagcgaat tgatggagtt gttctgcgga tatgggttct
    12241 gcgccaatac cagcgacgcg ccagcaggaa agatccagtt cagcgagatc tttttcattc
    12301 acgcggcgct ggcacaattc atagccaaac ggcggtgcaa cggaaacggt gccacgattt
    12361 ttactgatca atttaagcca ttgcagagga cgcatggcaa aatcctgagt gcgcaaataa
    12421 tctactgaaa gctgcgtggc gacgggggtc aggagaaagc cgaccagtcc catatcatgg
    12481 tagaaaggca gccaggagac gcagcggtcg ctagggcgta atttaatccc atcgtggctt
    12541 atagcacgta gattagccat cacttcgtga tgggtgataa tgacgccacg gggaaaacgg
    12601 gtgctgccgg aggtgtactg gaggtaggcg atatcgtttg gaactggacg ctggagcaca
    12661 acatcggctt ccggtaatgc cttaaaccag gcgtggctta aaacatgtaa ttcggggttg
    12721 ttgttatgcg tcgcggcatt gaccagtggc aaccactcat caccagtgat aatggctgcg
    12781 ggctggcaac ttgccagtaa accccgcagt ttggcgctcc aggaatcccg ctgacctacg
    12841 cccattggaa tcgccaacgg gacggcgact aagccggcat actggcaggc aaaaaaagcc
    12901 tctacaaacc cgctactcgt ttcggcaatc agcgccacgc gatcgccttt tttcaggttc
    12961 agcgataaca accttttcgc accagcttcg gcacgcgctt ttaacgtctg atattccagt
    13021 tgatcttcaa gttggcaacg tctgtcataa aaattcattc cggcgctact cagagcggcg
    13081 tagtccaaag catcaaccag cgttggaaaa tcggcatagc gcataggtag ggaatgcgta
    13141 aagattttat tagacatata caccacgaaa aaattggata tcaaaaataa aatgacaata
    13201 atgcggtgga ttatataggc ataaaagata aatgag
//
LOCUS       DAZKHK010000133        13075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKHK010000133 DAZKHK010000000
VERSION     DAZKHK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.134"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..261
                     /locus_tag="ACHOS5_004109"
     CDS             <1..261
                     /locus_tag="ACHOS5_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778837.1"
                     /translation="SFFSIETIDKINKENIKEIKLKNETTYIYLPNPTILITTTSTPE
                     RQFSISVLNDDNKENIPTDTAPCFTIGIPDEYTKAIELLNIK"
     gene            428..1351
                     /gene="yicL"
                     /locus_tag="ACHOS5_004110"
     CDS             428..1351
                     /gene="yicL"
                     /locus_tag="ACHOS5_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP9778838.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLLLLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1355..2173)
                     /gene="nlpA"
                     /locus_tag="ACHOS5_004111"
     CDS             complement(1355..2173)
                     /gene="nlpA"
                     /locus_tag="ACHOS5_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP9778839.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2395..2688
                     /gene="yicS"
                     /locus_tag="ACHOS5_004112"
     CDS             2395..2688
                     /gene="yicS"
                     /locus_tag="ACHOS5_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP9778840.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2729..3919)
                     /gene="nepI"
                     /locus_tag="ACHOS5_004113"
     CDS             complement(2729..3919)
                     /gene="nepI"
                     /locus_tag="ACHOS5_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP9778841.1"
                     /translation="MSEYIEENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4130..4582)
                     /gene="yicN"
                     /locus_tag="ACHOS5_004114"
     CDS             complement(4130..4582)
                     /gene="yicN"
                     /locus_tag="ACHOS5_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP9778842.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNMLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4635..5969)
                     /gene="adeQ"
                     /locus_tag="ACHOS5_004115"
                     /gene_synonym="yicO"
     CDS             complement(4635..5969)
                     /gene="adeQ"
                     /locus_tag="ACHOS5_004115"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP9778843.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQAGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6144..7910
                     /gene="adeD"
                     /locus_tag="ACHOS5_004116"
     CDS             6144..7910
                     /gene="adeD"
                     /locus_tag="ACHOS5_004116"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9778844.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(7957..9348)
                     /gene="uhpT"
                     /locus_tag="ACHOS5_004117"
     CDS             complement(7957..9348)
                     /gene="uhpT"
                     /locus_tag="ACHOS5_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP9778845.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9486..10805)
                     /gene="uhpC"
                     /locus_tag="ACHOS5_004118"
     CDS             complement(9486..10805)
                     /gene="uhpC"
                     /locus_tag="ACHOS5_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9778846.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10815..12317)
                     /gene="uhpB"
                     /locus_tag="ACHOS5_004119"
     CDS             complement(10815..12317)
                     /gene="uhpB"
                     /locus_tag="ACHOS5_004119"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP9778847.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQHDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(12317..12907)
                     /gene="uhpA"
                     /locus_tag="ACHOS5_004120"
     CDS             complement(12317..12907)
                     /gene="uhpA"
                     /locus_tag="ACHOS5_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP9778848.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELITAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         3515 a         3544 c         3134 g         2882 t
ORIGIN      
        1 tcttttttct ctattgaaac gattgataaa attaacaaag aaaacatcaa agaaataaaa
       61 ctaaaaaatg agacaacata tatttatctt cctaatccaa ccattttaat aaccacgaca
      121 tcaacacctg aacgacagtt ttctatttct gttctgaatg atgacaacaa agaaaatata
      181 cctaccgata ccgcaccttg ttttacaatt ggtattcctg atgaatatac taaagccata
      241 gagttattga acattaaata attaaatttc atagtgagtt tttttaaata ccatatgaat
      301 aattgttata tgtcattatt tcctccattc actaaaactg attgattact ctactccctc
      361 accgtcatcg gtagtggtat ccttaacgta tattgatgaa aagacaaaac agtatgcaga
      421 gggaaaaatg ggttccacca gaaaggggat gctgaacgtt ctgattgccg ccgtgttgtg
      481 gggaagttca ggggtctgcg cgcaatacat catggagcaa agccagatgt cgtcgcagtt
      541 tttgactatg acgcgtttga tattcgccgg tttgattctg ctgacgctat catttgttca
      601 tggtgataaa atcttttcta ttattaacaa tcacaaagat gccattagcc tactgatttt
      661 ttccgtggtt ggcgcgctaa ccgtacagct cacttttttg ctaaccatcg aaaaatcgaa
      721 cgcagccacg gcaacggtgc tgcaattcct ctcaccgact attatcgtcg cctggttctc
      781 actggtgcgt aaatcccgcc cgggcattct ggttttctgc gctattttga catcgctgat
      841 cgggactttt ttattggtga cacacggtaa tccgacgtca ttatcgatct ctcctgccgc
      901 gttattctgg gggattgcct cggcatttgc tgctgcattc tataccacct atccctcaac
      961 gctgattgcc cgctatggca cgttaccggt cgtcggctgg agtatgctca ttggcggtct
     1021 gctcctgctg cccttttatg ccagacaagg gacaaacttt gtcgttaacg gcagtttgat
     1081 tctggcgttt ttttatttgg tggtcattgg tacgtccctg acatttagtc tgtacctgaa
     1141 aggagcacaa ttaattggcg gtccaaaagc cagcatttta agctgtgcag aaccattaag
     1201 tagcgcgctg ctctctttgt tgttgctggg gatcaccttc acattgccgg actggctggg
     1261 aacgctgctg attctgtcat cggtgatttt gatttcaatg gattcccgcc gccgcgccag
     1321 aaaaataaat cgtccggcgc ggcatgagtg agaattacca gccaggcacc gcgccaccgt
     1381 taaagatggt ttctgccgct ttagcgactt cgggtgattg ataagattgc agaaattcct
     1441 tcacgttttc tgcattctta ttatcttccc gtgccaccag aatattcaca tacggcgaat
     1501 ttttatcttc aataaatacg ctgtcgttaa ccggagaaag tcctgtttgc tggatgtaag
     1561 tggtgctgat aatcgctaca tccactttcg ggtcgtccag tacacgtggc aactgtgccc
     1621 cttccagctc cattatctgc aaatggcgtg gattatcggt tatatccaac gcggtaggta
     1681 ataacccttt tccctctttc agagtaatta gcttctcttt ttgcagcagt aaaagcgcgc
     1741 gaccaaggtt agtcgggtcg ttggggatcg ccactgtcgc accttctttt atttgcgcca
     1801 ctgttttgat ttttttggaa tagcccgcca tggggaagac aaacgtattt cccaccgcta
     1861 ccagtttata accatgcgcc tgattatctt gttcaagaaa gggtcgatgc tggaagacgt
     1921 tcgcatcaag ctcgccatga tttgtcgcat cgttcggtag caacgaaccg ctaaatccta
     1981 ccagttcaac atcgagacca tatttctctt ttgctacctt tttagcgact tccgcgacat
     2041 cttgttctgc gccattaata acgccaactt taatatgctt ttcatcgctg ctgctctggt
     2101 cgcaacctgc cagcagaact cctgccagta ataatgcggc ccccgcccgt agatgatgtg
     2161 ttgtcagttt cattttttta tccttttatt aatgattacc tgactatagc ggtaaggtcg
     2221 ctgcagtata aaaaacgaaa cgctatcgat aagaataaaa aggaataaaa gtggaatata
     2281 aagtctggca cttcttactg accacgcaaa cacctttcgt acagcatgat gagagcggca
     2341 aaagcaaact acacttatgc ttcattcgat gcacatttgc agaaggttgc cgctatgaag
     2401 ccaacgatgc tactcatgat taccgtattc ctgatttttc cagccatttc tcaggccgag
     2461 tcgccattca gttctctaca gtcggcaaaa gagaaaacca cggtattgca agatttacgt
     2521 aaaatttgca cgccacaggc gtcattatca gatgaagcgt gggaaaagtt aatgttgtca
     2581 gatgaaaaca ataaacaaca tatacgcgag gccatcgtgg cgatggaacg taataatcag
     2641 agcaattatt gggaagcgtt gggtaaggta gaatgcccgg atatgtaaga acggcaacaa
     2701 ccactgacca acgcttcttt acctgatatc aggatttctt cattttcacc tttgcagtca
     2761 ccaacaatgc agtcagcaac atcaatgtgc cggacaacat cagcggcgaa gtcagaccaa
     2821 tgttatccaa cgcataaccg ccgattgccg caccgcaggt attggcaagc tgaataacgg
     2881 ccacctgaat agatccagcc ttttctgcct gatcggccaa tgagcgagtg atccacgttg
     2941 accaaccaac gggaattaaa gcaaaggtca gcccccagat aatcgccaca ccagtagcaa
     3001 caattttatc gcttccccac agcgtcagta ccagcgcgct cagggcgagc acaaacggtg
     3061 cgcccgctaa ggccagtttc accgaacgtt tgagaataaa cgacgaaagc gatgtcccga
     3121 caaagctggc gataccaaaa ctcagcagca ccagggttaa gccatcaaca ccgaatcccg
     3181 ccagattcat atacactggg cgaatataag tgaaaaaagc aaactgtccg gcaaaggaca
     3241 taaagatggc gatcatcccg gccatcactc ccggacgttg taataagcgg aaagtatttt
     3301 gtttctgatg cgaaggttca cctggcagcg aaggcagtga cttgataatc cagaaaatac
     3361 acagcacgcc catcgccgcc gccgcattaa agacattgcg ccaaccgata agctcgccta
     3421 aaaaactgcc caacggcgcg gcaatcacca gcgcaataga aaccgcgccg aagatcaccg
     3481 acagcgcctt cggcaccgta cgcggcggta ccagacgcat ggtcagcgac gccgacatcg
     3541 cccagaaccc gcccagcgcc agccccagac aggcgcgacc gattaacagc agactgaatg
     3601 agttggcaaa ggaaaccagc aaacaggaaa gcgtcagcaa aacggcgaac agaatgacaa
     3661 cgtagcggcg gtcagtggcc tgaatggtct gggtaataaa caaactggca aacatcgcca
     3721 caaaggcggt cacggtcacc gattggccgg caaccccttc cgaaatgcct aaatcctggg
     3781 ccattggcgt caacaaactg acaggcaaaa actcaacgat aatcagacag gcgacgcaaa
     3841 acgccaccga gaaaacggct gaccagttcg gtcgggtgat agcatccgcg ccgcggtttt
     3901 cttcgatata ttcactcatg gtgttacccg tggcatgttt cagagggaaa gccgaccact
     3961 ttaacattga aatgtgtgac gtatttaacg tttttgcaac ttttacggtg ttgcttgagc
     4021 tggagttaag atcgaaacgg aacagggcaa agattaccgg atgcggtgtg aacgtcttat
     4081 ccacccacca ggtcaggcgc ggtctgtagg ctgataagac gaagcggttt taatgcagcc
     4141 agtgaacgcc ctcggcaggc tgaaagaacg cgttatagcg catctgaaaa gcattgatat
     4201 cctgcatttc aggttcggtt tcgcgcagaa aacccagcgc cagcctgacc tgagcatcag
     4261 taatcggcgc ggcaaggcgg gctttttgta aaatccgatg ccattgcagc atgttctgtt
     4321 cgctaccatc gacaatgaca atttgccaga tgagcaacac ctgcgcggcg ttttgcaggt
     4381 gcgactcatc cgggcgatgc aaataacgta aaaattcgtc accggctgac tttcccattt
     4441 gatcgaccat cgactccacg ggtacgacat cgatgttcag ccgatcgctg agacggcgaa
     4501 tcgcttttct ggccccggat gtcagcaccc gaaaaccaac cacaaacact accgccagcg
     4561 tggcgagcat tatccagatc atgagttctc ctgcgtaatc agaactcatg atatctggaa
     4621 accctcgcca gttactaatc caccagaata atcttcagtg caaacagagc tgcgaccacc
     4681 accacacaca ggttcagatc gcgccagcgc ccggtgcaca ctttcatgat gcagtacgac
     4741 ataaagccta gtgcaatccc ttcggtgatc gagaaagtaa agggcatcat caccgtggta
     4801 ataaacgcag gcaccgattc ggtaaaatca tcccagttaa cgcgtgccag gctcgatgtc
     4861 atcaatacgc caacaaagat taacgctccg gcggttgcgt aaggaggaac catcgccacc
     4921 agcggtgaga agaacataac taacaggaac ataacgccga ctacgacagc cgtcagcccc
     4981 gtgcgaccac cgacagccac acctgaagta ctttcaatat aggcggtaac agacgaggta
     5041 ccgataaacg cacccgccac cgaactgacg ctatcaacat acagcgcctt attcatattg
     5101 gggaatttac cgttactatc tattaagcca gctttatcag tcacaccaat taatgtccct
     5161 gatgaatcaa ataagttgat cagcataaag gagaaaatga taccggcgag ttcaagtgat
     5221 aacgcaccgc tcaaatccac ttcaccaatg acgccgctaa tatcaggcgg aatggaatag
     5281 acaccgctaa aatgaacatc accgaaaaat aatccacagc aagacgtcac cacaatagaa
     5341 acaagcaccg cggcatgaaa atgacgtgat gacaacacgg ttataataaa aaaccctaaa
     5401 atacctaaca acacgccgtg agaacttaaa tcgccaatca tcaccagggt gtctttattg
     5461 gcgacaataa cgccagtatt ttttaatccc attaaggcga taaataatcc gattccgctg
     5521 gtgataccaa tacgtaaact taatggaatg ttggagatca tccagtaccg gatacgaaac
     5581 agcgtgagca aaaatagccc aactgcgccc cagaatatcg cgcccatccc ggcctgccag
     5641 gagataccca tcgcccccac gaccacgaag gcaaagaagg cgttcaggcc cattgccgga
     5701 gccagtgcca cgggtaagtt agcaaatacc cccatcgcaa tactgccgat accggcaatc
     5761 aaacaggtgg taacaaacac cacttttggg tccatttgtg ccgcgccgag gatttgcggg
     5821 ttcacaaaaa cgatgtacac catggttaaa aaagtggtca tccccgcaat caattctgtg
     5881 cggacggtgg tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta
     5941 ctcacgtaat cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct
     6001 tgcttttgta aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac
     6061 attaatggat attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt
     6121 gataaaatca aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta
     6181 gccgggctga ataccaggaa ttgttagccg tttcccgtgg agacgctgta gccgattata
     6241 ttattgataa tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg
     6301 tgattaaagg acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc
     6361 agcggattga tgcccgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata
     6421 ttgaatccag catgatgacg ccggtcacct ttgaaactgc taccctgccg cgcggcctga
     6481 cgaccgttat ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct
     6541 ggtttgcccg ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg
     6601 tacccgccct ggaaggctgc gatgttaatg gtgccagttt tacccttgaa cagatgctcg
     6661 cctggcggga ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa
     6721 ttagcgggca gaatgcgctg ctcgataaac tggacgcatt tcgccacctg acactggacg
     6781 gtcactgccc gggtttgggt ggtaaagaac ttaacgccta tattgctgcg ggtattgaaa
     6841 actgccacga aagttatcag ttggaagaag gacgccggaa attgcaactc ggcatgtcat
     6901 tgatgatccg tgaagggtcc gcagcccgca atctcaacgc gctggcaccg ttgatcaacg
     6961 aatttaacag cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc
     7021 atgaaggaca catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtaccgctgc
     7081 atgtggcata tcgcgtagcc agctggtcga cggcgcgcca ttttggtctg aatcacctcg
     7141 gcttactggc acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca
     7201 cggtgcagca ggtactggtg aaaggcgagc cgatcgatgc acaaacctta caggcggaag
     7261 agtcggcgag actggcacaa tccgccccgc catatggcaa taccattgat cgccagccag
     7321 tttccgccag cgactttgcc ctgcaattta cccccggaaa acgctatcgc gttattgagg
     7381 taatccataa cgaactgatc acgcactccc gctccagcgt ctacagcgaa aacggttttg
     7441 atcgcgatga tgtgtgcttt attgccgttc ttgagcgtta cggacaacga ctggctccgg
     7501 cctgtggttt gctcggcggc tttggcctga atgaaggtgc gctggctgcg acggtcagcc
     7561 atgacagcca taatattgta gtgatcggtc gtagcgcaga agagatggcg ctggcggtca
     7621 atcaggtgat tcaggatggc ggcgggctgt gcgtggtccg taacggccag gtacaaagtc
     7681 atctaccgtt gcccattgcc gggctaatga gcaccgacac ggcgcagtca ctggcggagc
     7741 aaattgacgc cctgaaagcc gccgcccgtg aatgcggtcc gttaccggat gagccgttta
     7801 ttcagatggc gtttctttcc ctgccagtga tccccgcgct gaaactaacc agtcaggggc
     7861 tgtttgatgg tgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaaaag
     7921 cccggcgtca tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat
     7981 ttttttctcg cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat
     8041 acaaccaatc gcggcgatat ccagcgcggc gaaggtgcct gcccagccgg taaggccaaa
     8101 taccggcgtc ccatcggcaa tcattcccag acctaacttg gcaaagctgt caccaatcag
     8161 gtaagcgaag gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc
     8221 aacagcagcc acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag
     8281 agaagccagg tagatatatt cgttgctggc gtgttgatac acaccgagcg tggcgataat
     8341 cagcgccagc gcgatacagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca
     8401 gccccacagc agcgtaccga ccagcgcacc agcttcaaac agcgtaaagc cctgaattgc
     8461 cactgcttta gagagtttca gttcctggaa cgcatacacg gttgaccact ggtcgatacc
     8521 aatacgcacc acatagagga aaatgttggc aaagcacagc agccagatca ctttgttttt
     8581 cagcacatac tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttatc
     8641 ctcttcactg atctcctcgc caaacagctc ttcagctttg ccgaggccat aagattccgg
     8701 ggagtcgctg ccgtaacgca gaccgataaa accaacaatc agcgcgataa tcgacgggaa
     8761 aataaacatg ccgatgacat gaccatcgaa caggtaattt gcgccgaaca gcgccacacc
     8821 tgctgcgcct gcaccaccaa ggttgtgaga aatgttccag aaaccgagga atgtcccgcg
     8881 tttacgacgc ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtactctg
     8941 gaaaaagccg cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc
     9001 catactggcg ctgaagccca gcatacaaat ggcagacagg atcagcatga acggcaggaa
     9061 ttgtttggtg tttttgccgt cggcgtagta agaaaccagc gttttaccca cgccataagt
     9121 gatggagaag cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgagat
     9181 catgtcgttc tgcgcgatgt taaagttctt gcgaatcagg tacatcgtaa ggtagccgat
     9241 aaagaccacc aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag
     9301 cggaaggtcc agggtcggct tgcgaacctg gtttaagaaa gccagcatgg gttactcctg
     9361 aaatgaatac ctgcgtgagg catgcattgt aaaaatcagc agcaaatcag gcctgagata
     9421 gcgtccaggc aaaacctgga aaatttctta gttttgccgg atatgaagtg aaaaggtgag
     9481 atgcatcacg cttctcgcgg tgtctgggcg ttcaaaaagg gcagtaatag cagtgcggaa
     9541 atcccggcgg cgatggcgat aaccacaaaa aatccgctcc agtgccaggt atcgagtact
     9601 ttcgccagcg gccaaccagc aagcgacgca ccaagataag caaacaagcc gacaaatccc
     9661 gtcgccgccc ctgccgcctc tttgtgggaa cactctgccg ccgccatgcc gattaacatc
     9721 tgcgggccaa agacaaaaaa accaatggtg aagaagcagg ttgcctgcat cacgtagctg
     9781 gcaaatggca tcagccacag cgagccgact gaaagcaaaa ttccggcggc gaaaatcaaa
     9841 ttcatcggcc cgcggttgcc gttaaacagt ttgtccgaac cccaacccgc taccagcgca
     9901 ccgataaatc cgcccagttc aaacattgtc actgccgtat tcgccgtgac cagatcgacg
     9961 cccagcgtct cggacatata caaattgccc cagtcgttta tcgccgcccg gaccacatag
    10021 accagcacat agcaaaacga aagcagccag atatacggat tcagcaacac atatttggtg
    10081 aggatctctt tacgcgtcag ccctgcccct tcttgttgtt gagcaatctc cagcgcgtcg
    10141 tgccgccatt caccaaccgc cggtaaacct aacgcctgcg ggcgatcgcg cagccgccag
    10201 cagagaaaaa tccccacgac gatcgccata caacccgcaa tcatcatccc ggcacgccag
    10261 ccgtaatgca gcgcgctcgc tgccatcaca atgggaatga gtgcgccgcc gacgttatgc
    10321 gccgtgttcc ataatgccca ccaaccgccg cgctcggtac gagaatacca ggcggttaac
    10381 agacgcgcac acaccggtga accccagccc tggaaaaagg cgttcaacac ccaaagcacg
    10441 gcaaacgccc atagcgacgt cgagaagcca aacagaatgt tgataatgcc cgtggcaata
    10501 agccctatcc ccataaaata acgggcattt gagcgatcgc tgacaatgcc ggagacaaac
    10561 ttcgacacac cgtaggtaat gtaaaacagg gtcgctaaca ggccaatatc gctacggctg
    10621 agcacgccgt tagcaaggat ttctggtacg gcggcgttaa aacttttacg cgtgaagtaa
    10681 aacagcgcgt aacccagcca gatggtcagc agaatatgcc gacgccagta gcgatagcgg
    10741 gcatcaattt catgtttatc agtcattaat ggcgcatcgg cgggcgcttt cagaaacggc
    10801 aacatcatcg aaccttagac ataacgttga ggtagagaaa cgctgacacg cgtgccgtgc
    10861 aggcaggaga tggtcaatgt gccacccagc gccgttacgc gctcgcgcat tccggtaagg
    10921 ccaaaaccgt gttgcccgga atctggcggt aaaccgctgc catcgtcttc aatcaccagc
    10981 attaaccgct catcatgctg ccagccttgc agcgtgaccg cgctggcatc ggcatgtttc
    11041 acaatgttgt tcaacccttc ctggcagacg cgaaacagcg tcacgcgctg gttttcgctt
    11101 aacgctgatt catcgattcg ccattcgaga tgactgacaa tgccgcgccc ttccagctcc
    11161 atttcccgca tcagtgagcg gatggcctgc tccagggtga gatcatccag ctggcgcgga
    11221 cgtaaccgcc ctaacaagcg gcgcaccgcg tcgtaaacgc ccaacgacaa ctgttcgatg
    11281 agctgcccgc tctgtttcac gctggcgtta tccgccgcga gccgctggac aatgcccgct
    11341 tgagttcgaa tagcagtaat ggtctgaccg atatcatcgt gcagctcacg tgccacatca
    11401 cggcgcacgc tctcttcggt ttccagcaac cgttcagcga gatgctgatt gcgcgccagt
    11461 tccttttgca gcgactggtt aagttcacgc aaccgctgga tgccagcgcc cagtaacaac
    11521 cctgtcagac tttgcaccag cagcgagagc aataaatcca ccggatgatc gcgccaggtt
    11581 tgactggcga tcagcgcgat ggcgttcatc aatgtcgcaa tcagcgctcc ttgccaacca
    11641 tagtgccagg ccagcgcgat aatcggcagc gccagacaga atggcgtaaa gcgcgacagt
    11701 tcgtccggca atcccaactg gagccagaga ctgataacaa acagcagcaa gtaccagacc
    11761 agatgccgcc cacgccagtt gattggttga gaaaccagtg acgggccgag cggcagccag
    11821 gtgttattgg cgagatagtg ccagaacacc agacatatcg gggccagcgt caggccgcca
    11881 gttaaagtca gcagtagcgc attccacgac tctttgccgt gccaaagcca gggcagcgac
    11941 tgcaacaacg ccgccgccgt taatgccgca ccctgtaaca gcaaggtgcg ccagtcacgt
    12001 tgatggcgat agcgcgagat cagcgccacg ggcagtaacg tcagtaaact accgatcatc
    12061 aataaggaga aatgggttaa accgaccgcc tgcgttaacc agtaaatcag cagccactcc
    12121 gcgcccagca acaccggcca gtatccgcgc gggcattgca gcattagccc tagccgcagg
    12181 ccaaacggaa ataacagtac cgccatatca gggcgctcaa ccaggtgcag gctgatactc
    12241 cacaggcaaa accatgcggc agagaagata aaaaagcagg caataacggt aattaagcgg
    12301 gagaacaacg tcttcatcac cagccatcaa acatgcggcg cgccagttca acgtcgttac
    12361 tgacgcccag tttttccatc aggttggcgc gatggacatg taccgttttc ggtgacaagc
    12421 ccagttcggc agcaatctct ttcaccgcca ttccttgcgc cagtttttcc gccacctggc
    12481 gttcgcgttt ggttagcggg tcctggcgac cggatgccag tttaatggca atatccggcg
    12541 tcagataaca gccgcccgtg gcaaccgtat gcaccgcagt aatgagttca tccgggctac
    12601 agcgtttgga gagaaagccg cgcgcccccg cgttaagcgc ctgctcaacc agcgccggac
    12661 tgtcatgaac ggagagcata attgtcgcca tgcctttcgg cagctggctt agaagctcca
    12721 gaccggagat atcaggcatg gagatatcgc aaatacacac ctgcacaccg cgccccggca
    12781 gccccgccag cgcctcgcgc cccgaaccaa actcggcaac tacctgcaaa tcaggttcca
    12841 gccccagcag ctgcgcaaag ccggagcgga cgataaggtg atcgtctata agggcaacgg
    12901 tgatcatggt cttgtcctgg cgggtaataa aacgcgctta ccttaacgat aagcgcgcca
    12961 ctgttcaagc cttgagcggt tactgaaaaa agctgcgctc ctgttgcttg tgaaaagaaa
    13021 tgatgccttc aaggtttggt atatcgacta atcattaaaa acgctggcag catta
//
LOCUS       DAZKHK010000134        12951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKHK010000134 DAZKHK010000000
VERSION     DAZKHK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.135"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            251..2956
                     /gene="malT"
                     /locus_tag="ACHOS5_004121"
     CDS             251..2956
                     /gene="malT"
                     /locus_tag="ACHOS5_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP9778849.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2999..4015)
                     /gene="rtcA"
                     /locus_tag="ACHOS5_004122"
     CDS             complement(2999..4015)
                     /gene="rtcA"
                     /locus_tag="ACHOS5_004122"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP9778850.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGTGEFTVAHPSCHLLTNIAVVERFLPVRFGFIETDGVTR
                     VSIE"
     gene            complement(4019..5245)
                     /gene="rtcB"
                     /locus_tag="ACHOS5_004123"
     CDS             complement(4019..5245)
                     /gene="rtcB"
                     /locus_tag="ACHOS5_004123"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP9778851.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSACAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5440..7038
                     /gene="rtcR"
                     /locus_tag="ACHOS5_004124"
     CDS             5440..7038
                     /gene="rtcR"
                     /locus_tag="ACHOS5_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP9778852.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKERPDSPGAVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGTEAENIDLFDRMQLENVIAVCRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWETMQDQHSSS"
     gene            complement(7020..7778)
                     /locus_tag="ACHOS5_004125"
     CDS             complement(7020..7778)
                     /locus_tag="ACHOS5_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP9778853.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(7795..8625)
                     /gene="glpG"
                     /locus_tag="ACHOS5_004126"
     CDS             complement(7795..8625)
                     /gene="glpG"
                     /locus_tag="ACHOS5_004126"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP9778854.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8670..8996)
                     /gene="glpE"
                     /locus_tag="ACHOS5_004127"
     CDS             complement(8670..8996)
                     /gene="glpE"
                     /locus_tag="ACHOS5_004127"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP9778855.1"
                     /translation="MDQFECINVTDAHQKLQEKEAVLVDIRDPQSFAMGHATQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9186..10691
                     /gene="glpD"
                     /locus_tag="ACHOS5_004128"
     CDS             9186..10691
                     /gene="glpD"
                     /locus_tag="ACHOS5_004128"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP9778856.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDEDGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS"
     gene            complement(10745..11350)
                     /locus_tag="ACHOS5_004129"
     CDS             complement(10745..11350)
                     /locus_tag="ACHOS5_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778857.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEITF
                     SFPLLVVRISLQELQLELMVEYALFFSVMGGIGWQFSRRMPPELKNRLHCWLVFSPAY
                     FWLILSNFILYITPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILSLGPLFNYSQ"
     gene            complement(11353..12120)
                     /locus_tag="ACHOS5_004130"
     CDS             complement(11353..12120)
                     /locus_tag="ACHOS5_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778858.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPKQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITAQINAWAFNNQSILKERARQAIEN
                     PLKQPEQGTQYQIWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(12123..12833)
                     /locus_tag="ACHOS5_004131"
     CDS             complement(12123..12833)
                     /locus_tag="ACHOS5_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778859.1"
                     /translation="MELVDTLFASLSGTDPFTGVDITIANCKSTYWDEGIVQQLINQV
                     LDEGEKFAGAVGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDLY
                     ISDVPDVQCDLNTTNDFTVQFTAMLNPDERVIIAKRPLKKCDAWIEDVYIFQVFKEAW
                     QFQNGNSLRGFRDKQAELKLYARHYSVENCTEESCWDCNYCIRPSFSLSRSAIIRLNA
                     ANARFVYQPFTRDQRARG"
BASE COUNT         3224 a         3464 c         3225 g         3038 t
ORIGIN      
        1 aagaaaccct tacttaaatg aaactgatga acataaggaa aataccgtac aacccagtat
       61 tcacgctgga tcagcgtcgt tttaggtgag ttgttaataa acatttggaa ttgtgacaca
      121 gtgcaaattc agacacataa aaaaacgtca tcgcttgcat tagaaaggtt tctggccgac
      181 cttataacca ttaattacga agcgcaaaaa aaataatatt tcctcatttt ccacagtgaa
      241 gtgattaact atgctgattc cgtcaaaatt aagtcgtccg gttcgactcg accataccgt
      301 ggttcgtgag cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat
      361 cacaagtcct gcgggctacg gaaagaccac gctcatttcc cagtgggcgg caggcaaaaa
      421 cgatatcggc tggtactcgc tggatgaagg tgataaccag caagagcgtt tcgccagcta
      481 tctcattgcc gccgtgcaac aggcaaccaa cggtcactgc gcgatatgtg agacgatggc
      541 gcaaaaacgg caatatgcca gcctgacatc actcttcgcc cagcttttca ttgagctggc
      601 ggaatggcat agcccacttt atctggtcat cgatgactat catctgatca ctaatcctgt
      661 gatccacgag tcaatgcgct tctttattcg ccatcaacca gaaaatctca cccttgtggt
      721 gttgtcacgc aaccttccgc aactgggcat tgccaatctg cgtgttcgtg accagctgct
      781 ggaaattggc agtcagcaac tggcatttac ccatcaggaa gcgaaacagt tttttgattg
      841 ccgtctgtca tcgccaattg aagccgcaga aagcagtcgg atttgcgatg acgtttccgg
      901 ttgggcgacg gcactgcagc taatcgccct ctccgcccgg cagaatactc actcagccca
      961 taagtcggca cgccgcctgg cgggaatcaa tgccagccat ctttcggatt atctggtcga
     1021 tgaggttttg gataacgtcg atctcgcaac gcgccatttt ctgttgaaaa gcgccatttt
     1081 gcgctcaatg aacgatgcac tcatcacccg tgtgaccggc gaagaaaacg ggcaaatgcg
     1141 cctcgaagag attgagcgtc aggggctgtt tttacagcgg atggatgata ccggcgagtg
     1201 gttttgctat cacccactgt ttggtaactt cctgcgccag cgctgccagt gggaactggc
     1261 ggcggagctg ccggaaatcc accgtgccgc cgcagaaagc tggatggccc agggatttcc
     1321 cagcgaagcg attcatcatg cgctggcggc aggcgatgcg ctgatgctgc gcgatattct
     1381 gcttaatcac gcctggagtt tgttcaacca tagcgaactg tcgctgctgg aagagtcgct
     1441 taaggccctg ccgtgggaca gtttgctgga aaatccgcag ttggtattat tgcaggcgtg
     1501 gctgatgcaa agccagcatc gctacggcga agttaacacc ctgctggccc gcgctgaaca
     1561 tgaaatcaag gacatcagag aaggcaccat gcacgcagaa tttaacgctc tgcgcgccca
     1621 ggtggcgatt aatgatggta acccggatga agcggaacgg ctggcaaaac tggcactgga
     1681 agagctgccg ccgggctggt tctatagccg cattgtggca acctcggtgc tgggtgaagt
     1741 gttgcactgc aaaggcgaat tgacccgctc actggcgtta atgcagcaaa ccgaacagat
     1801 ggcacgccag cacgatgtct ggcactacgc tttgtggagt ttaatccagc aaagtgaaat
     1861 tctgtttgcc caagggttcc tgcaaaccgc gtgggaaacg caggaaaaag cgttccagct
     1921 gatcaacgag cagcatctgg aacagctgcc aatgcatgag tttctggtgc gcattcgtgc
     1981 gcagctgtta tgggcctggg cacggctgga tgaagccgaa gcgtcggcgc gcagcgggat
     2041 tgaagtcttg tcgtcttatc agccacagca acagcttcag tgcctggcaa tgttgattca
     2101 atgctcgctg gcccgtggtg atttagataa cgcccgtagc cagctgaacc gtctggaaaa
     2161 cctgctgggg aatggcaaat atcacagcga ctggatctct aacgccaaca aagtccgggt
     2221 gatttactgg caaatgaccg gcgataaagc cgccgctgcc aactggttgc gtcatacggc
     2281 taaaccggag tttgcgaaca accacttcct gcaaggtcaa tggcgcaaca ttgcccgcgc
     2341 acaaatcttg ctgggcgagt ttgaatctgc ggaaattgtc ctcgaagaac tcaatgaaaa
     2401 tgcccgcagt ctgcggttga tgagcgatct caaccgtaac ctgttgctgc ttaatcaact
     2461 gtactggcag gccggacgta aaagtgacgc ccagcgtgtg ttgctggacg cattaaaact
     2521 ggcgaatcgt accggattta tcagtcattt tgtcatcgaa ggcgaagcga tggcgcaaca
     2581 actgcgccag cttattcaac tcaatacgct gccggagctg gaacagcatc gcgcacagcg
     2641 tattctgcga gaaatcaatc aacatcatcg gcataaattc gcccatttcg atgagaattt
     2701 cgttgaacgt ctgctaaatc atccggaagt accagagctg atccgcacca gcccgttgac
     2761 ccaacgtgaa tggcaggtcc tggggctgat ttactccggt tacagcaatg agcaaattgc
     2821 aggcgagctg gaagtggcgg caaccaccat caaaacgcat atccgcaatc tgtaccaaaa
     2881 actcggtgtc gcccaccgtc aggccgcggt acaacacgcg cagaaactgc tgaagatgat
     2941 gggatatggg gtgtgagttt agccggataa tgcgtcggat ccggcttaca tctttacatc
     3001 attcaatgct cacccgcgtt acgccatctg tttctataaa accaaaccgc accggcaaga
     3061 aacgctccac cacggcgata ttggtcagca gatggcatga gggatgggcg accgtaaatt
     3121 cccccgtgcc cgccagcgcc atcggtagca ccagttggtc ggcgagatat tcccccaccg
     3181 ccgccgggct tgccaggtag cgtttcacct ctttcaccaa ctgtgccgcg accacctcgg
     3241 cactgacgcg cttttcaccg acgacaaaaa agcgttcggt gatattttca ctttcgactt
     3301 caagcgagac ggtattaccc ggcccctggt cgcgcggcag gttatgaata ttctgttcat
     3361 gcaaggaaaa actacccgcc agtgtagcga tttcacgctc agcaacatgg cgcggcacac
     3421 cagccagtag cacctctcca cgcatctgca caatgttccc gcgctcgcca agttgcaagc
     3481 tattaaacga tgccaccggt gagacttccg ttgccaccac gccgcctccg gcaggataaa
     3541 aaccgtgacg taacagcgtg gtttgctgat gaactcctat tttcgccagc agcggctcca
     3601 gcacccggcg gataaaatcc gcaggcgggg ccgacgggtt atcggtgccg ccgctcactt
     3661 caacacgcga aggtccatcg gcaaaccaca gcgcgggcag caccgtttgc agcaccagcg
     3721 tacaacttcc ggcgctaccg atagcaaagc ggtaatcgcc gccgcgcacg gtgccaggtc
     3781 ggaagaccag acgctgcgac cccagctcag cgccttccac cgttgcccta caaatttccg
     3841 cagccgcttt taccgcggtc agatgttggc gcaacagccc cggtttcgcc cgcccggcac
     3901 gaatgccggt gatggtaaat ggctggccgg ttatcatcga caggctcagc gccgagcgca
     3961 ggatctgccc gccgccttcg ccctgtgcgc catccagcgc aatcatcctt ttcatcattt
     4021 atccttttac gcacaccacc tgacgcaggg tatagataac ttccaccaga tcgctttgtg
     4081 ccgccatcac cgcatcaata tctttatacg ccatcgggat ttcgtcgatc acctcggcat
     4141 ctttacggca ctccacatgc gcggtggcac ggatttgatc ttccacactg aacagttttt
     4201 tcgctttagt acggctcatt acccgcccgg caccgtggct gcacgaacag aacgactctt
     4261 catttcccag cccacggacg ataaagcttt tcgcccccat cgaaccggga ataattccat
     4321 attgaccagc acacgcagac accgcgcctt tacgcgtcac atagatctct tcgccaaagt
     4381 gctgttcttt ttgcacatag ttgtggtgac agttgatctc ttccatcgcc agcgtttgtg
     4441 gctgttttac cgttttctgc gtaacgctct gcaacgccgt taccacgttt tccatcatcg
     4501 catcgcggtt aaggctggca aaaagctgcg cccaggccac ggctttcagg taatcatcaa
     4561 agtattccgt accttccata aagtacgcca ggtcacgcga tggcaacgtt tcaagcgttt
     4621 cctgcatctc tttttgcgcc agatcgataa agtaagttcc gatggcgtta ccaattccgc
     4681 gtgaaccgga gtgcagcata atccacacct gatccgactc atcaaggcaa atttcaataa
     4741 agtggttacc cgtacccagc gttcccaggt gtttatagtt attagtgtta aggaaacgcg
     4801 gatatttttg cgttaaccac tgataaccgg cctccagctc agcccatttc gcatcgacgt
     4861 taacaggcgg attttcccat gcgcctttat cacgtttaca acgcccggta gtacgtccgt
     4921 gcggtacggc cgtttcaatc gcctgacgca gctctgccag gttttcaggc aggtccgccg
     4981 ccgttaacgc ggtacgcagt gcgttcattc cacagccaat atccacgccc accgccgccg
     5041 gaataatcgc ccctttggtc gggatcacgc taccaatggt ggaaccttta cccaggtgta
     5101 catcaggcat taccgcaata tgtttgaaaa taaatggcat cttcgcggta ttaataagtt
     5161 gctgacgcgc atcggcttct accggcacgc ctttggtcca catttttacc ggagcatttt
     5221 cagtggtcag taattcgtaa ttcattttgt ttttctcttt tcgttgtttg ctgtcctgat
     5281 aattgcaatt gcaaccgtcg tgccagaaaa tcaaataagc atcttaattt tttaattcat
     5341 tgtttttaaa aatattatat ttttacgtcc gtaaacggag atttcccgca aagccgattt
     5401 accgataatg aaatctcgtc ttttataagg atatctaaga tgcgtaaaac agtggctttt
     5461 ggctttgtcg gaacggtgct ggattatgcc gggcgcggca gtcagcgctg gtcaaaatgg
     5521 cgtccgacac tctgtctatg ccagcaagaa tcgttagtta tcgatcggct ggaattattg
     5581 cacgacgccc gctcgcgctc gctattcgaa acgcttaaac gcgatatcgc cagcgtttcg
     5641 ccagaaacag aagtggtggg cgttgagata gaactgcata acccttggga tttcgaagag
     5701 gtttacgcct gcctgcatga tttcgcccgt ggttacgcgt tccagccaga aaaagaagac
     5761 tatttaattc acatcaccac cggtacccac gtcgcgcaga tttgctggtt tctgctggca
     5821 gaagcgcgtt acctgcccgc ccggttgata caatcttcac caccgcgcaa aaaagagcgg
     5881 cccgatagcc ctggcgcagt gacgattatc gatctcgatt taagccgtta taacgccatc
     5941 gccagccgct ttgccgagga acgccagcaa acgcttgatt ttcttaagtc cggcattgcc
     6001 acgcgtaact cccacttcaa ccgcatgatt gagcagattg aaaaagtggc gataaaatcc
     6061 cgcgcaccga tcctgcttaa cggcccgacc ggtgcaggta agtcatttct ggcgcgacgc
     6121 atctttgaat taaaacaggc acggcatcag tttagcggcg catttgtaga agtgaactgc
     6181 gccaccctgc gcggcgatac cgccatgtcg acgctgtttg gtcatgtgaa aggcgcgttt
     6241 accggggcgc gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttattt
     6301 cttgatgaga ttggcgaact gggcgcagac gaacaggcga tgttgctgaa agccattgaa
     6361 gagaaaacct tttatccgtt tggcagcgat cgccaggtga gcagtgattt tcagctgatc
     6421 gccggaacgg tgcgcgattt gcgccaactg gttgccgaag gcaaatttcg cgaagatctg
     6481 tacgcgcgga tcaatctctg gaccttcacc ctgccggggc tacgccagcg ccaggaagat
     6541 attgaaccta acctggatta tgaagtggag cgccacgcct cactcaccgg cgacagcgtg
     6601 cgttttaaca ccgaagcacg gcgcgcctgg ctggcctttg cgacatctcc ccaggcaaca
     6661 tggcgcggta actttcgcga actttctgcc agcgtcacgc ggatggcaac ctttgccact
     6721 agcggacgca tcactctgga cgtggttgaa gatgagataa accgtctgcg ctataactgg
     6781 caggagagtc gcccctccgc gcttacggcg ttgctgggta cagaggcaga aaacatcgac
     6841 ctcttcgacc gtatgcaact ggaaaacgtt attgccgttt gtcgtcaggc aaagtcgctt
     6901 tctgctgccg gacgacagct ttttgatgtt tcgcgccagg gtaaagccag cgtcaatgac
     6961 gcggatcggc tacgcaaata cctggcgcgt tttggtctga catgggaaac aatgcaggat
     7021 cagcacagct ccagttgaat atgatggtcc ttcagcacct gcatcacgct cgcaggcggc
     7081 atcgtgtcgg tgtagacagc atcgaccatg ctgatgctac ccatattgac cattgcgtta
     7141 cggccaaatt ttgagtggtc gacaaccagc ataacgtggc gcgagttctc aatgatggcg
     7201 cgtttggtgc gaacttcgtg gtaatcgaac tccagtagcg agccgtcgct atcgatgccg
     7261 cttatcccca gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgttgcttcg
     7321 ccaatgatcc cgccatcgcg gctgcgtaat tcgccaccgg caagaatgat acggaaatcc
     7381 tcttttacca tcagcgtgtt agcgacgttg agattgttgg tcacaatgcg taaattgctg
     7441 tgattaagca gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc
     7501 gagccattgg gaatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg
     7561 gtggccttgc gatcgtgcca cggcgtatta accgaactgg aaggcagcgc cgcaccgcca
     7621 tgatggcgca ggatcaggtt ttgctctgcc agctcgttga gatcgcggcg aatagtctgc
     7681 gggctaacgg agaaatgctc taccagctcc tcggtgctga cataaccctg ctgtttaacc
     7741 agttcgataa taccgttgtg acgttgtgtt tgtttcattg ataaatccct ggaattattt
     7801 tcgttttcgc gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaacccggc
     7861 gatgtgtgct ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca
     7921 gatcaacgca aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc
     7981 gccacgtagc cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca
     8041 cggcccgctg aatttttgct gcacatagcc gcttaacagg gcgctaatga gagtaatgac
     8101 aattagctta ccgctaccga ggcgtttttc caccgcaccg ccgagatacc accaccagag
     8161 caggttaaag aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca
     8221 gaactcaaat ttcagcgtcg gatcgaatgg ccaggccagc cataacatca cttcctgatc
     8281 gccgagaatt tgcatggcga taaacaccac cacgcaggcg atcatcatca cccaggttac
     8341 cggacctgcg cgttcacgca aggcggcaaa gaaaggataa cggcgataat gcaggccact
     8401 gccggtatgg cccgcctgcc agctggccgc cagataacgc ggatctgccg ggttttcgag
     8461 aaaacgcgcc agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc
     8521 gctttggtta tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa
     8581 cgcctgcgcc actcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta
     8641 ttccacacaa aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac
     8701 tggcgctgcc aggcttcaaa gccgccatca atgctgtaga ccacatcgta gccctgttgc
     8761 agcagatact gcgccgcgcc tttgctgcta ttgccgtgat aacacatcac catcaccggt
     8821 gtgtcaaagt cgttatcacg cataaacgcg cccagtgtgt cgttagttaa atggaaagcc
     8881 tgggtcgcat gccccattgc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct
     8941 ttttcctgca acttctggtg cgcgtcggta acgttaatac attcgaactg atccatgcgt
     9001 ctctctttct ttacaaacaa gtgggcaaat ttaacgcaca gtttacgtcg aagcggcaga
     9061 taaacgccat aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc
     9121 gattctttgc taatatgttc gataacgaac atttatgaac tttaacgaaa gtgaatgagg
     9181 gcagcatgga aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg
     9241 cggcagacgc cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt
     9301 gcgcgacctc ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact
     9361 atgaattccg cctggtcagc gaggcgctgg ctgaacgtga agtgctactg aaaatggccc
     9421 cgcatatcgc cttcccgatg cgttttcgcc tgccgcatcg tccgcatctg cgcccggcgt
     9481 ggatgattcg cattggtctg tttatgtacg atcatctggg taagcgcacc agcctgccgg
     9541 gatcaaccgg tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat
     9601 tcgaatattc tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg
     9661 tggtgcgtaa aggcggtgaa gtgcttactc gtactcgtgc cacgtctgct cgccgcgaaa
     9721 acggcctgtg gattgtggag gcggaagata tcgataccgg caaaaaatat acatggcagg
     9781 cgcgcggcct ggttaatgct accggcccgt gggtgaaaca atttttcgac gacgggatgc
     9841 atctgccttc gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgtg
     9901 tgcataccca gaagcaagcc tacattctgc aaaacgaaga taaacgtatt gtcttcgtga
     9961 tcccgtggat ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggcgatc
    10021 caaaagcagt gaagattgaa gagagtgaaa tcaattacct gttgaaagtg tataacacgc
    10081 actttaaaaa gcagttaagc cgggacgata tcgtctggac ctactccggc gtgcgtccgc
    10141 tatgtgatga tgagtccgac tcgccacagg ccattacccg tgattacacc cttgatatcc
    10201 atgatgaaga cggcaaagcc ccgctgctgt cggtattcgg cggtaagctg accacctacc
    10261 gcaaactggc ggagcacgcg ctggaaaaac tgacgccata ttatcagggc attggtccgg
    10321 cgtggacgaa agagagcgtg ctgccgggtg gtgccattga aggcgaccgt gacgattacg
    10381 ccgcccgcct gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacgcgc
    10441 gcacttacgg cagcaacagc gaactgttgc tcggcaatgc aggggcgata agcgacctcg
    10501 gtgaagattt cggtcatgag ttctacgaag cggagctgaa atatctggtc gaccacgaat
    10561 gggtccgccg cgccgacgat gccctgtggc gtcgcacgaa acaaggcatg tggctaaatg
    10621 cggatcaaca atctcgtgtg agtcagtggc tggtggaata tacgcagcag aggttgtcgc
    10681 tggcgtcgta aggatgatgc ccggttgtga agtgcaaccg ggctatttga attccttaga
    10741 ctattcattg tgaataattg aataacggcc ccagagataa aatcgccaaa cttaagcacg
    10801 ttacccaatc gccccacttc ttcctatggc ttctattttt gctacgccac tggcaataat
    10861 atcccaacgc aaaaccaagc tgtgcactaa tcgggccgac aaaatcagtc cacggctgcg
    10921 gccaaaaagg ggaaaaattc aggggaagcc agacaaacgt cagaaagaag tttcggatat
    10981 tttccagcaa cgctgatttc tctggagtaa tataaagaat gaaattcgag agaattaacc
    11041 agaaataggc cggagaaaag accagccagc aatgcagtct gttttttaat tcaggaggca
    11101 ttctgcggga gaattgccaa ccgatgccac ccattacact gaaaaaaagt gcatattcaa
    11161 ccattaactc aagttgtaat tcctgaagag aaatacgcac aaccaggagc ggaaaggaga
    11221 atgttatttc tgccagaaaa gttaacgtga atataagtac ggctaaggca ataaccgtcg
    11281 gccataccaa taatggtacg ataacaaaac cgagaattac agggggaata taaagccgga
    11341 atttaatcat ttttattttc ctggtgattc ggatgatgcg tcatacatcg cgcataaaca
    11401 ctcatcaatc atccagtcgt cttcccagat ctgatactgt gtgccctgtt cgggctgctt
    11461 aagcgggttc tcaatcgcct gccgcgcgcg ttctttcagg atggattgat tgttgaaggc
    11521 ccaggcattg atttgggcgg taatggcttt atcgatatcg tcctggttat ctctgatata
    11581 accgtcacga tacgtattca ataaacaggc aagagacgct gccgaccaaa atccgggaac
    11641 tgaaataatg gcatcaagca atggcgaaag agcgatatcg gcacggaaaa acatttccag
    11701 cttgtactgt aattcgacag cctcatcccc ggatacccaa tcagtcatat actggcaagc
    11761 aaattgctgc aatatttccc gttcatcctc acgccagttt tctttactgg caaggtttat
    11821 acgccgtaga gaaaatactt cgtgaatatt gtcaaattgc ccctgcgcaa caaactccag
    11881 taaacgaggc agaaaataac gaacctcatc gctgttttgc ggatcaggtc ctggactact
    11941 attccaggca ttgattaagg aataaggaat cgctcgcaac gatgtattcc gtaatgcttt
    12001 ttgttcctgt tgactcagac aatattcaca acaaaccaca aattgcttcg gcgccggata
    12061 acgacgaaaa atatgatata tctgctcaat taatgcctgc atggcaggag tggggtgcat
    12121 aattaccccc tcgcccgctg atcgcgtgta aagggttgat agacaaaacg agcattcgcc
    12181 gcattcaggc gaataatggc actacgagag agggaaaagc tgggccgaat gcaataatta
    12241 cagtcccaac agctttcctc tgtgcaattt tccactgagt aatgacgcgc gtagagtttc
    12301 agttcagcct gcttatcgcg aaatccgcgt aaactgttac cgttttgaaa ttgccaggct
    12361 tctttaaaca cctgaaagat ataaacatcc tcaatccagg cgtcacattt tttaagcgga
    12421 cgctttgcta tgatcacccg ctcatccggg ttgagcatcg ccgtaaattg tacggtgaag
    12481 tcatttgtcg tgttcagatc acattgaaca tctggtacat cactgatata gaggtcaaat
    12541 ccaaagtcag caccacaggc acaaagacgg ctaatggtgg cggtatcgag ggaaaatccc
    12601 ggccatacat tgctggacgt taaaccaata tttaatgtga catcatatcg agataatccc
    12661 tcaagaccga cagcaccggc gaacttttcc ccttcatcca ggacctggtt aatgagttgc
    12721 tgaacaatgc cttcatccca gtatgttgat ttgcagttgg caatcgtaat atccacgcca
    12781 gtgaatggat cagtaccgga gaggcttgca aataacgtat caactaattc catttttatt
    12841 atccggctta atgcaacatt ttattcatcg gcacataacc tacattttcc accaggtttt
    12901 tcccctgtag aattaaaaat atttttttgt tttacattct gttcatccct a
//
LOCUS       DAZKHK010000135        12927 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKHK010000135 DAZKHK010000000
VERSION     DAZKHK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.136"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..571
                     /gene="tssB"
                     /locus_tag="ACHOS5_004132"
     CDS             71..571
                     /gene="tssB"
                     /locus_tag="ACHOS5_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9778860.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
     gene            630..2168
                     /gene="tssC"
                     /locus_tag="ACHOS5_004133"
     CDS             630..2168
                     /gene="tssC"
                     /locus_tag="ACHOS5_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9778861.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2186..3523
                     /gene="tssK"
                     /locus_tag="ACHOS5_004134"
     CDS             2186..3523
                     /gene="tssK"
                     /locus_tag="ACHOS5_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9778862.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDCIAQLCIRHPWGI
                     ANVLFDRDALTPGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVQLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGSCMIYAPGTMGDLKPELFAVLRT"
     gene            3520..4185
                     /gene="tssL"
                     /locus_tag="ACHOS5_004135"
     CDS             3520..4185
                     /gene="tssL"
                     /locus_tag="ACHOS5_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP9778863.1"
                     /translation="MKKDMDINIDALLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFDVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4198..5850
                     /locus_tag="ACHOS5_004136"
     CDS             4198..5850
                     /locus_tag="ACHOS5_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9778864.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWSVFISPSGVLRWAGAAAIVLAVA
                     ALLIYRRRQAWTEMTGDAGLSSLPPETYRQPVVLVCGGLSAHLSTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYTIMPGIHRDVAVLAGRLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERASSPWFICTGGEVQVATSTETTMPAQWIAQSGVQE
                     RSQRLCYLLKAESLMQWLNLNVLTALNGPEAKCPPLAMTVGLVPSLPAVDNNLWQLWI
                     TARTGLTPDIADTGTDDALPFPDALLRQLPRQSGFTPLRRACVTMLGVTTVAGIAALC
                     LSATANRQLLRQVGDDLHRFYAVPVEEFITKARHLSVLKDDATMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5908..6399
                     /locus_tag="ACHOS5_004137"
     CDS             5908..6399
                     /locus_tag="ACHOS5_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9778865.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6591..9236
                     /gene="tssH"
                     /locus_tag="ACHOS5_004138"
                     /gene_synonym="clpV"
     CDS             6591..9236
                     /gene="tssH"
                     /locus_tag="ACHOS5_004138"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9778866.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDALPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSALPETATADAPAKTLAEKQDDALLAVLNRFTEDVTEKARRGRIDPVF
                     GRDTEIRQMVDILSRRCKNNPILVGEPGVGKTALVEGLALRIAEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYARHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQDGMDSTELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVVR
                     RLRQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9248..11728
                     /locus_tag="ACHOS5_004139"
     CDS             9248..11728
                     /locus_tag="ACHOS5_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2345 domain-containing protein"
                     /protein_id="HFP9778867.1"
                     /translation="MGIQNFDHSHHKLKIRGLKSPVDVLTFTGHEQLSSPFRYDIQFT
                     SSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKNVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDAWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVLDMTD
                     ALAQLREAQSLVEALCSATEVAKAELADLQTQKVMMSEALEELKKSAMLLSAPEGIAQ
                     VTPKSLQLSAGENIISTSGKNSDFSVLKKFTVAAGETVSLFAQKLGIKIFAGKGKVEI
                     QAQGDEMLLDALKDIRISSSEGRILISAKNEIILTSGGGYIRIGDGTVECAAPDKIIE
                     RGAVWQKFSGQSISQAMQQWDSADFAVTPEILWQQTGKPAKNQKVQVTRGDGSVVEMT
                     TDEQGRLPIQSGLFVESIKIDLPDSQEN"
     gene            11748..>12927
                     /locus_tag="ACHOS5_004140"
     CDS             11748..>12927
                     /locus_tag="ACHOS5_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HFP9778868.1"
                     /translation="MTSTMHMDRADKNKVGTVEIEDLRVIPVIFLPGVMGSNLMDKKG
                     KSIWRYDDSMSLMGWSLPTSGPKERKRLLHPDRVEVDNRGRIPAPPDAQEKLIQLGQQ
                     YPEDPSDKEAMDNYTQAVREALDNMEPEAKLFGSRHDRGWGEVAAASYGSFLDALQTA
                     LYRDKPTRKDNTLSATYQKLLDEPLGLEYGPDSLDEEYLEVMKLYQFPVHVVGYNWLG
                     SNRLSAKRLSEEVEKIIKRYQEQKMKCHKVILVTHSMGGLVARYYSECLTEPGPGRDK
                     IYGIVHGVMPSIGAAATYTRMKRGTENPESGMAGAAGYVTSHVLGRNAAEMVAIGSQS
                     SGALELLPANDYGDDWLKVVDRDGSTLTFPRDLPVGEDAKKENCLYSELYLNRDNWWR
                     L"
BASE COUNT         3044 a         3482 c         3754 g         2647 t
ORIGIN      
        1 tcatccgata atcccctgaa tataattatg tcaataacca tcagaaaaag tggatgatga
       61 ggaaaaggat atggccgaca gtttccagaa tgaagttccc gccgctcgtg taaatatcaa
      121 gcttgatctg catacaggca atgctaaaaa gaaagttgaa ctccccctca agcttcttgc
      181 cgtaggcgat tacagtaacg gaaaagagca acgtccgctg tccgaacggg acaaggttga
      241 tatcaataaa aacaacttca acagcgtcat ggctgagttt tcgcctgcgg ttaatttaac
      301 agtagaagat acgctaaacg gaaacggtaa tgaacaaaat atcgcgcttg aatttaaaag
      361 cctgaaggat tttgaacccg aacaggtagc aaaaaatatt ccgcaacttc gtgtattact
      421 cgctatgcgc aatttactac gtgacctgaa atccaatctg ctggataacg caacgtttcg
      481 ccgtgaactg gaaaatattc tgaaagaccc gacgctcagt tcagaactgc gtgatgaact
      541 ggcgaaaatt gcgccgcagg agaacgttta acccgagcac gtattcaggt taaaccggag
      601 aagaaaggat cactgaaaag gaaatgctga tgtctgtaca acaagaacat gccacctctg
      661 aaactgcaac actcaccacc actgagtccg gcggcgttta tcagtccctg ttcgataaaa
      721 ttaatttaac cccggtctct tccattcagg aaatagattt atggcaaaac agcgaaacgc
      781 tggctgatgc ctcacccgat gagcgcgtga cagcagcgat tcacgttctg ctttcctgtc
      841 tggcgaaatc aggcgaggac gtggttaagc tcgacaagag cctgctggat tttcatatcg
      901 acgatctgga tcagaaaatc agtaaacagc ttgatgcggt catgcaccac cctgaattcc
      961 agaaagtcga gtcgctgtgg cgtggtacat ggttcgtcgt acagcgcact gattttcgca
     1021 aaaatgtcag aattgaattg ctggatatca gcaaagagca tctgcgtcag gatttcgacg
     1081 actccccgga aatcattcag agcggtttat atcgtcatac atacattcag gagtacgata
     1141 cgccgggtgg cgaacctgtt gcctcattaa tttccagcta tgaatttgat aacagcccgc
     1201 aggatattgc cctgctgcgc aatatttcca gagtgtctgc cgcttcccat atgcctttta
     1261 tcggttccgt cggaccgaaa ttcttcctta aaaattcgat ggaagaagtc gccgcgatta
     1321 aagatatcgg caactacttt gaccgcgcag aatatattaa atggaagtcg ttccgcgata
     1381 cggatgacag ccgctatgtg ggattagtga tgccgcgagt gctgggccgt ctgccctatg
     1441 ggccggacac ggtgccggta cgcagcttta actatgtgga agaagtcaaa ggcccggatc
     1501 acgaaaagta cctgtggaca aacgcctcgt tcgcctttgc cgccaatatg gtgaagagct
     1561 ttgtgaataa tggctggtgt gtgcagatcc gtggtccaca ggcaggtggc gcagtggccg
     1621 atctgcccat ccatctttac gatctcggca ccggcaatca ggtcaaaatt ccgtccgaag
     1681 tgatgatccc ggaaacccgc gaatttgaat ttgccaacct tggctttatt ccgctctctt
     1741 attataagaa tcgcgattac gcctgcttct tctcggcaaa ctccgcccag aaaccggcgt
     1801 tgtatgatac cgctgacgcc accgccaaca gccgtattaa tgcccgtttg ccctatattt
     1861 tcctgctgtc ccgcattgcg cattacctga aaatcatcca gcgcgagaat atcggtacca
     1921 ccaaagaccg ccgcgtgctg gaactggagc tgaatacctg gatccgcacg ctggtgacgg
     1981 agatgaccga tcctggcgat gaacttcagg cttcgcatcc actgcgcgac gggaaagtta
     2041 tcgtcgagga catagaggac aatccgggct tcttccgcgt cagactcttt gccgtgccgc
     2101 atttccagat tgaagggatg gatatcaacc tgtcactggt ttcccagatg ccgaaagcga
     2161 aagcctgacc gggcggtgat gtgatatgaa aattcatcga ccactgtggg ctgaggggac
     2221 attcctgtcc tctcagcagt ttcagcagca ggcccgctgg gaagcgttca gcaatgactg
     2281 tattgcgcag ctttgtattc gccatccgtg ggggatcgca aacgtgctct ttgaccggga
     2341 tgccctgacg cccggaaagc tgaaaacgca ggcagtacgc ctgcgttttg ctgatggcac
     2401 gctgatcgat tcagacgtga gtgacgttct gccgctcgcc tgcgatctgc gcgcgctgaa
     2461 gaacgacagc gcgattgttt tgctggcgct gccgcttgca catggtaacg gcggtaatct
     2521 cggtcagggt gagcagactg aaagaccgct gcgctatcgt caggaatggc aaaaggtgca
     2581 ggatatctac ggcagtgaca gtgaagatat ggccgttgag cgtcatgcgc tgtccctgcg
     2641 ctttgctcat gataacaatc aggattacat cacctgccca ctggcgcgtc tggtgcggga
     2701 tgttcagggc aactggacac aggatgagag ctatattccg ccgttactgg cgtttaacgc
     2761 ccatgacggg ctggtacagc gtctggacac attgctgttg cagctacggg cgaagtgcca
     2821 gcgtctgatg gcgatgcgcc gggaaagcaa ccagcgtatg gcggactttg ccgtcgccga
     2881 tgtctccctg ttctggctgc ttaacgcgct gaattccgcc gagccggtgc tgagcgattt
     2941 cctgcgctac ccggctgtcc accccgaact ggtctggcgg gagctggcga ggcttgccgg
     3001 tgcgctgctg acgttttccc ttgagcataa cgtcagcgcc gttccgccct atgtccacga
     3061 atcgccgtcc accgtctttc cgcccctgtt ctccctgctc agcgagctgc tggaagccag
     3121 cctgccgtcg cgggtgatcg cgctggatct ggcgtccctg ccgggcaacc gctggaaagc
     3181 cgacctgcac gatccgcgcc tgcgggaaga ggccgatttt tatctgtccg ttcgttcctc
     3241 actgcctgcg catcaggtac tgcatcagct accgctggtc tgcaaaatcg gtgcgcccga
     3301 tgatgtcacg ctgcttatca acgtggcgct gaacggtgtt caactggtgc cgcttacctc
     3361 ggttccggcg gcgctgccgc tgcggctgga gaaccagtat tttgcgctgg atatgcacag
     3421 tgacgcggct aaatcgatgc tggagtccgg gagctgcatg atttatgcgc cgggaaccat
     3481 gggcgacctg aaacctgaac tgtttgcggt gctgcgcaca tgaaaaaaga tatggatatc
     3541 aatatcgatg cgctgctgcg cgacacgttt ctgacggtgg tggaactgcg tcagggaacc
     3601 accgtgcgtc acggtatcga gctgtatcgc cactgtcagc ggcaggtgga actggtccgc
     3661 gaacgtctga aagatgccgg attcagtcgg gagaacgtgg agcacataac ttacgcgcag
     3721 tgcgccctgc tggacgaaac ggtgctcagc cgtagtggca tggacgacgg tcaggccatc
     3781 tggatgaaaa acccactcca gtcgcatttc ttcaacaccc tgcaggccgg tgagctgctg
     3841 tacgagcgga tgaagcaggt gctacaggaa cccgcccccg cgcaggccgt gctgacctgc
     3901 tttcaccgtg tcctgctgct gggatttcgc gggcgttatc aggatccggc agccccggag
     3961 cgcgaccagc ttatttcgac gctcaacggg caggtcgcgc cctttgacgt gctgccggaa
     4021 acggcggtac tgaacgtgcc gttatccacc cgtcagcacc ctttgctgca ttcgccgttt
     4081 ttctggctgg taacactggc gctgctactg gcgggcgtct ggtgggggct acaccactgg
     4141 ctgaatgtac tggtagatga actgctgccg caaagcctgc gttaaaggga gtcgtgagtg
     4201 aggaacacgc tgaaacaggc catcgtgctg tggggaatgg tgttactgct ggtgctgtgg
     4261 tcggtgttta tcagtccgtc tggcgtgctg agatgggccg gtgcggcggc tatcgttctg
     4321 gcggttgccg cgttgttgat ttaccggcgc aggcaggcgt ggacggagat gaccggcgat
     4381 gccgggttgt catcgctgcc gccggaaacc taccgacagc cggtagtgct ggtctgtggc
     4441 ggtctgtcgg cgcacctgtc cactgacagc ccggtccgcc aggtttcaga agggctgtat
     4501 ctgcatgttc ctgatgaaga acagcttgtg gcgcaggtgg agcgattgct gacccttcgc
     4561 ccggcgtggg catcgcagct tgccgtggcg tataccatca tgcccggcat acaccgggat
     4621 gtggcggttc tggccggacg gctgcgacgg ttcgcccaca gtatggcgac ggtgcgtcgt
     4681 cgggcaggcg taaacgtccc ctggcttctc tggagcgggc tgtccggctc gccgttgccg
     4741 gaaagagcga gttcaccgtg gtttatctgt accggcggcg aagttcaggt agcaacatcc
     4801 acagagacca ccatgcccgc gcagtggatt gcacaatccg gcgtacagga gcgcagtcag
     4861 cgactctgtt acctgctgaa agctgaaagc ctgatgcagt ggctgaatct taatgtgctg
     4921 acggcactga acggcccgga ggcgaaatgt ccaccactgg cgatgaccgt ggggctggtc
     4981 ccctcgttgc ctgcggtgga taacaacctg tggcagttgt ggatcaccgc cagaaccggc
     5041 ctgacgccgg atatcgcgga caccggcaca gacgatgcgc tgccgtttcc ggatgccctg
     5101 ttacggcagt tgccgcgtca gtcgggcttt accccgctgc gacgagcctg cgtgaccatg
     5161 ctgggcgtca ccaccgtggc gggtatcgcc gcgctgtgcc tgtcagccac ggcaaatcgc
     5221 cagttattac ggcaggtcgg tgacgatctg caccggtttt atgccgtccc ggtggaggaa
     5281 tttatcacca aagcccgtca cctgtcggtg ctgaaagacg atgcgaccat gctcgatggg
     5341 tattaccggg aaggagaacc actgcgcctc ggtctggggt tataccccgg cgaacgcatc
     5401 cgccagccgg tattacgcgc cattcgcgac tggcgtccgc ctgaacaaaa aatggaggtg
     5461 acggcttcgc ttcaggttca gaccgtgcgt cttgacagta tgtcgctgtt tgacgtcgga
     5521 caggcccgcc tgaaagacgg ctcgacaaaa gtgctggtgg acgcactggt gaacatccgg
     5581 gcaaaaccgg gctggctgat cctcgtggcc ggatataccg atgccaccgg cgatgaaaaa
     5641 agcaatcagc agttatcgct gcggcgtgcc gaagcggtgc gcaactggat gctgcagacc
     5701 agcgacatcc cggccacctg ttttgccgta cagggactgg gcgagagcca gcctgcggcg
     5761 accaacgaca cgccacaggg ccgggcagtc aaccggcgtg tcgaaatcag tcttgttccg
     5821 cgttctgacg cctgtcagga cgtgaaataa aacataccgc cggaagaagg cggtgcttca
     5881 atcacactaa caaggagagt aattctcatg gctattcctg cttatctctg gctgaaagat
     5941 gacggcggcg cggatatcaa aggttccgtg gacgttcagg ggcgcgaagg tagcatcgaa
     6001 gtggtggcgc tggatcacga tgtgtacatc ccgaccgaca ataacaccgg caaactgacc
     6061 ggtacccgta ctcacaagcc ctttacgttt accaaagaaa tcgatgcctc cagcccgtat
     6121 ctctacaaag ctgtgaccac cggacagacc ctgaaaacgg cagaatttaa gttttaccgc
     6181 atcaacgatg ccggtcagga agtggagtac ttcaacatca cgcttgataa cgtcaagctg
     6241 gtcagagtcg ctccgcttat gcacgacatc aaggatcctt ccagagagaa gcataaccac
     6301 ctggaacgta ttgagttccg ctacgagaaa atcacctgga cttacaaaga cggcaacatc
     6361 attcattccg actcgtggaa tgagcgtcct tccgcctgat aactgcggca gacaggaaac
     6421 tgtctgcctt tctgttgtgt gctttgtcca gtgccgcagg caccggtact gagcaaagca
     6481 tacccacaat ctggtgaaac ctgatgggcc tgagtgaggt aacggctatg ggcggtagcg
     6541 tgcctgaacg cggatcgcag gaatacagaa cacgataagg aaacccttct atgacaggaa
     6601 atcaccccgc cgcgctgctg cgtcgcctta acccatactg tgcacgggcg ctggacgccg
     6661 ccgcctcgct gtgtcagacc cgcgcccatg cggaaataac cattgaacac tggctgctga
     6721 aactgctgga gcagggagaa ggcgatatca cggtgattgc ccgccgctat gaatgggata
     6781 tcgacacgct ctggcagtct ctgctggcgc atctggatgc cttaccccgc ccggtccgcg
     6841 aacgtccaca gctttctgaa ccgctggcgg cgcttatcag acaggcgtgg ctgattgctt
     6901 cgctggaagg cgacgacccg caaatccgca gtcagcacct gctgatggcg ctgacagaaa
     6961 aaccgatgct gcccgcctgt aatgacctgt gggtattgct gagtctgagc cgcgtgcagc
     7021 ttgagcggct gcgtcccctg ctggatgcgc agtcggatga atgtccggca cgtcagccac
     7081 aggtcaccga accactgacc tctgcactgc cggagacggc aacggcggac gcaccggcaa
     7141 aaacgctggc ggagaaacag gatgacgccc tgctggcggt gcttaaccgc tttaccgaag
     7201 acgtgacgga aaaagcccgt agagggcgaa tcgacccggt attcgggcgc gacacggaaa
     7261 ttcgccagat ggtcgatatc ctctcccgcc gctgtaaaaa caacccgatt ctggtgggtg
     7321 aaccgggggt gggtaaaacc gcgctggtgg aagggctggc gctgcgtatc gctgaaggca
     7381 acgtaccgga cagcctgaaa acggtgcata tccgcacgct ggacctcggt ctgttacagg
     7441 ctggcgcggg tgttaaaggt gaatttgaac agcggctgaa aaacgtgatt gatgcggtac
     7501 agaaatcacc ggagccggtg ctgctgttta ttgatgaagc ccataccatt atcggcgcgg
     7561 ggaatcaggc gggcggcgcg gatgcggcga acctgctgaa accggcgctg gcaaggggag
     7621 agctgcgcac catcgcggca accacgtgga gcgaatacaa acagtatttt gagcgcgacg
     7681 ccgcgctgga acgccgcttc cagatggtga aagtcgacga gccggatgac gataccgcct
     7741 gcctgatgct gcggggactc aaggcccgct acgcccggca ccacggcgtg catatgctgg
     7801 acagcgccat tcagaccgcc gtgcgcctgt cgcgccgcta tctgaccgga cgccagttgc
     7861 cggacaaggc ggttgatttg ctggataccg ccggagcaag agtccgcatg agtctcgaca
     7921 ccctgccgga gccgctgaca cagcttcatg cgcgtctggc cgcgctggat atcgagcggg
     7981 aggcgattga acaggacagc gtgttttatc ccgaaaccag cccggagcgg ctggcggaac
     8041 tgaccgattt gcgtgatgag ctacaggcag aagccgggca tctggaagcg cagtatcagc
     8101 aggaaaaggc actggcgcag cagattatga cgctgcgtca ggacgggatg gacagcactg
     8161 aactgcaaca gcaactgcga acgcatcagg gctttgcacc gctgctggcg ctggatgtgg
     8221 acgcccgcgc cgtcgccacg gtggtggcgg actggaccgg catcccgctc tcatccctgc
     8281 tcagggacga gcagagcgac ctgctcagta tggaacaaag ccttgaaaac cgcgtggtcg
     8341 ggcaaagccc ggcgctctgc gccatcgcac agcggctgcg ggcggctaaa accggcctca
     8401 cgccggagaa cggcccacag ggggtattcc tgctgaccgg ccccagcggc accggcaaaa
     8461 ccgaaactgc gctcacactg gccgacaccc tgtttggtgg tgaaaaatcc cttatcacca
     8521 ttaatctttc ggaatatcag gaaccgcata ccgtttccca gttgaaaggt tcgcctccag
     8581 gctacgttgg ctacggtcag ggtggcgtac tcaccgaagc cgttcgcaaa cgcccttaca
     8641 gcgtggtgct gctcgacgaa gtggaaaagg cgcaccgcga cgtaatgaac ctgttctatc
     8701 aggtatttga ccggggcttt atgcgcgacg gcgaaggccg ggaaattgac ttccgcaaca
     8761 ccgtgattct gatgaccgcc aatctgggca gcgaccacat catgcagctg ctggaggaaa
     8821 aaccggacgc cacggacgca gacctgcatg aactgctgta ccctctgctg cgcgagcact
     8881 tccagcctgc actgatggcg cgttttcaga cggtgattta ccgcccgctg ggacaggagg
     8941 cgatgcgcgc cattgtggaa atgaaactgg cgcaggtggt tcgtcgtctt cgtcagcact
     9001 atgggctgga aacggaaatc aacgacagcc tgtatgacgc cctgaccgcc gcctgcctgc
     9061 tgcctgatac cggtgcgcgt aatatcgaca gcctgcttaa ccagcagatc ctgccggtct
     9121 taagccagca gttgctggca cagcaggccg cgcaccgtaa accagcccaa ctacggcttg
     9181 gctgggatga ggaggacggg attgtactgg agttcgctac ggaagagatg caataacgga
     9241 ggctgtcatg ggtattcaga atttcgacca ctcacaccac aagctcaaaa ttcgcggcct
     9301 gaaaagcccg gtggacgtgc tgacttttac aggccacgaa cagctcagct cacccttccg
     9361 ctacgacatt cagtttacca gcagcgataa agccatcgcg ccggaatcgg tgctgatgca
     9421 ggacggcgca ttcagcctga cggcaccgcc cgtacaggga atgccggtac agactgccct
     9481 gcgcaccctg cacggcgtca ttaccggctt taaacatctc tcatcgtcac aggacgaagc
     9541 ccgttacgaa gtgcgccttg aaccccgtat ggcgctgctt acccgcagcc gccagaacgc
     9601 catctaccag aaccagacgg tgccgcaaat cgtcgaaaaa atcctgcgcg agcgacacca
     9661 gatgcgcggt caggacttcg tgtttaacct caaaagcgaa tacccggccc gcgaacaggt
     9721 gatgcagtat ggcgaggacg acctgacgtt tgtctcacgc ctgctgtcgg aagtgggtat
     9781 ctggttccgc ttcgccaccg acgcccggct gaaaatcgaa gttatcgaat tttacgacga
     9841 ccagagcggc tatgagcgcg ggctgacgct gccgcttcgc catccttctg ggctgtttga
     9901 tggtgaaacg gaagccgtat gggggctgaa cacggcatac agcgtggtgg agaaaaacgt
     9961 caccacgcgg gactacaact accgcaccgc cacagcggag atgatgacag agcagcatga
    10021 cgccaccggg ggcgataaca ccacctacgg cgaagcctac cactatgctg acaacttcct
    10081 gcaaaaaggc gataaagagg cagcggaaag cggcgcgttt tatgcccgta tccgccacga
    10141 acgctacctg aatgaacagg ccattctcaa aggccagagc accagttcac tgctgatgcc
    10201 ggggctggaa atcagggttc agggcgacga cgcgccagcg gtgttccgca agggtgtgct
    10261 gattaccggc gtgacggcca gcgcggcgcg tgaccgcagc tatgaactga cgttcacggc
    10321 gataccctac tcagaacgct acggctaccg cccggcgctt atcccccgcc cggtaatggc
    10381 cggcacgctc ccggcgcggg tcaccagcac ggtgaagaac gatatttatg cccatatcga
    10441 caaggacggg cgctaccggg tcaatctcga ctttgaccgc gacgcatgga aaccgggtta
    10501 tgaaagcctg tgggtacgcc agtcgcgccc gtatgcggga gacacctacg gcctgcacct
    10561 gccgctgctg gcgggaacgg aagtcagtat cgcgtttgag gaaggcaacc cggaccgacc
    10621 ttatattgca ggcgtaaaac acgactcggc acacactgac catgtgacca tccagaacta
    10681 caagcgcaac gtactgcgta ccccggccaa caacaaaatc cggctggatg acgaacgcgg
    10741 gaaggagcat atcaaggtca gcaccgaata cggcggcaag agccagctaa acctcggtca
    10801 tctggtggat gccgggaaac agcagcgcgg ggaaggcttt gaactgcgta ccgatatgtg
    10861 gggcgcagtg cgggcgaaga aagggatttt tatcagcgcc gatgcgcagg acaaggcgca
    10921 gggtcaggtt ctggatatga ctgatgcact agctcaactt cgcgaggcac agtctttggt
    10981 agaggcacta tgtagtgcta ctgaagtggc aaaggctgaa cttgcagatt tgcagactca
    11041 gaaagtaatg atgtcagagg ctctggagga actcaaaaaa tcagcgatgt tgctttcggc
    11101 tccggaagga atagctcagg ttacgccaaa aagcctgcag ctgtccgcag gagaaaacat
    11161 cattagcacc agtggaaaaa attcagattt cagcgtgttg aagaagttta ccgttgctgc
    11221 tggagaaacg gtaagtctgt ttgcccaaaa gctggggata aaaatttttg ccggtaaggg
    11281 aaaagtagag attcaggcgc agggagatga aatgttgcta gatgcgctga aagatatccg
    11341 catcagcagc agtgaggggc gaatccttat tagcgcaaaa aacgaaatca ttctgaccag
    11401 cggcggcgga tatattcgta ttggtgatgg tacggtggaa tgcgcagccc cagacaagat
    11461 catagagcgt ggggccgtat ggcagaagtt cagcggccag agcatcagtc aggcgatgca
    11521 acagtgggat tctgctgatt ttgctgtcac accagaaata ctctggcaac aaactggcaa
    11581 acctgcaaaa aatcaaaagg tacaggtcac acggggtgat ggctcagtgg tggaaatgac
    11641 caccgatgaa caaggacgat tgcccataca gtcagggctt ttcgttgagt caataaaaat
    11701 tgatctgccg gatagtcagg aaaactgagt gcatacaggg ggaaattatg acatcgacga
    11761 tgcatatgga cagggccgat aagaataaag ttggcaccgt tgaaattgag gatctacggg
    11821 ttattccggt tatttttctg cccggagtca tgggttcaaa ccttatggat aagaaaggaa
    11881 aatccatctg gcgatatgat gactctatga gtctgatggg ctggtcacta cctacatcag
    11941 gaccaaaaga gaggaaaaga ttattgcatc ctgacagggt ggaggtcgat aatcgtggga
    12001 gaattcccgc acctccggat gcccaagaaa aacttattca gttaggtcaa caatatccgg
    12061 aggacccatc cgataaagaa gcaatggata attacactca ggcagtacgt gaagctctgg
    12121 ataatatgga gccagaagcg aaattatttg gttcacggca tgatcgggga tggggagaag
    12181 tggccgctgc aagctacggc tcttttcttg atgcgctaca gacagcactg taccgggata
    12241 agcccacgag aaaagacaat accctgagtg cgacctatca gaaattactt gatgaaccac
    12301 tggggctgga gtatggcccg gattcactgg atgaagaata tcttgaagtt atgaaactgt
    12361 atcagtttcc ggtacatgtt gtgggctata actggctggg ttccaacagg ctttcggcaa
    12421 aaagactaag tgaagaagtc gagaagatta ttaagcggta tcaagagcag aaaatgaagt
    12481 gccataaggt tattctggtc actcactcaa tgggcggact ggtggcacgg tattattcag
    12541 aatgtctgac cgaacccgga ccgggccggg ataaaattta cggtattgtt cacggtgtca
    12601 tgccatccat cggcgcggca gcaacctata cccgcatgaa gcgtggtact gaaaatccag
    12661 aatcgggtat ggctggtgct gcaggatatg tcaccagcca tgtacttggc cgcaacgcag
    12721 cggaaatggt ggctattggc tcccagtctt ccggcgcgct ggagttactc cctgccaatg
    12781 attatggtga cgactggctg aaggttgttg accgtgacgg tagtacatta acattcccca
    12841 gagatctgcc ggtcggagag gacgctaaga aagaaaattg tctttatagc gagctttatc
    12901 tgaacaggga taactggtgg aggctga
//
LOCUS       DAZKHK010000136        12735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKHK010000136 DAZKHK010000000
VERSION     DAZKHK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.137"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1038
                     /locus_tag="ACHOS5_004141"
     CDS             <1..1038
                     /locus_tag="ACHOS5_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9778869.1"
                     /translation="MLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERILTDRENGE
                     KGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYAGLSYKDF
                     GSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTDFFGMVEG
                     LNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQSGDGKGER
                     AEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDFGLRPSIG
                     YSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVLAHGLNTD
                     DQLAVGIVYQF"
     gene            1634..2485
                     /gene="hchA"
                     /locus_tag="ACHOS5_004142"
     CDS             1634..2485
                     /gene="hchA"
                     /locus_tag="ACHOS5_004142"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP9778870.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2593..3951)
                     /gene="hprS"
                     /locus_tag="ACHOS5_004143"
     CDS             complement(2593..3951)
                     /gene="hprS"
                     /locus_tag="ACHOS5_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP9778871.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRIKIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVVIILCSLLSPWLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLNALSLNKEVENLLDYLEYLSDEKEIRF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(3951..4622)
                     /gene="hprR"
                     /locus_tag="ACHOS5_004144"
     CDS             complement(3951..4622)
                     /gene="hprR"
                     /locus_tag="ACHOS5_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP9778872.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4755..5168
                     /gene="hiuH"
                     /locus_tag="ACHOS5_004145"
     CDS             4755..5168
                     /gene="hiuH"
                     /locus_tag="ACHOS5_004145"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP9778873.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5277..6281
                     /gene="msrP"
                     /locus_tag="ACHOS5_004146"
     CDS             5277..6281
                     /gene="msrP"
                     /locus_tag="ACHOS5_004146"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP9778874.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6282..6917
                     /gene="msrQ"
                     /locus_tag="ACHOS5_004147"
     CDS             6282..6917
                     /gene="msrQ"
                     /locus_tag="ACHOS5_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP9778875.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGELGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            7175..7825
                     /gene="zinT"
                     /locus_tag="ACHOS5_004148"
     CDS             7175..7825
                     /gene="zinT"
                     /locus_tag="ACHOS5_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP9778876.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8673..9893
                     /locus_tag="ACHOS5_004149"
     CDS             8673..9893
                     /locus_tag="ACHOS5_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9778877.1"
                     /translation="MPKKAKELSGLVVSRLKSEGMYAVGGVDGLYLRIRNQSRAWVLC
                     VAMGTRINNLGRIVPRRLNMGLGPYPEVSLAEARDKARELRKKIRNGINPLQEKHEQK
                     ARQEILARKKKTFAECCEEVLEVKDSEMKNKKHLAQWRSTLETYAYPFIGKKAVSEIT
                     KVDLLAILEPIWLTKNETASRLRGRIETVIDYAKAKEYFEGDNPAAWKGMLKPLLPQP
                     SKVQITKHHAALPYNQLGSFMKELRERSGVSPRALEFAILTAARSGEIRGAEWSEIDL
                     EGKTWTIPASRMKAAKEHRVPLSDAAVALLKALPRFKGINFVFPATRKGQLSDTALLA
                     VLKRMGYTDLTQHGFRSTFRDWAGETTNYPREVIEHALAHQLANKAEAAYQRGTLWPK
                     RVALMDDWAGYCVS"
     gene            10038..10484
                     /locus_tag="ACHOS5_004150"
     CDS             10038..10484
                     /locus_tag="ACHOS5_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9778878.1"
                     /translation="MSKLDWFNNVENRPHPWEGMGLTWLTTALNVPDVKAAINFYTEV
                     MKMVVISELEGDGGDVLFARIRYRGTNFTINKEGWDSDLKSPNTSGAMPPFIFYMYVD
                     DMITLVNEMVEAGSTVLNEPAEMFWGDLKARVKDPFGFIWDIAQKL"
     gene            complement(10627..12090)
                     /gene="pilV"
                     /locus_tag="ACHOS5_004151"
     CDS             complement(10627..12090)
                     /gene="pilV"
                     /locus_tag="ACHOS5_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFP9778879.1"
                     /translation="MIKKKGFTLLEVTIVLGIGTLIAFMKFQDMRNDQEAVLADNVGT
                     QIKQLGEAVNRYISIRYDKISTLSSSNNQSSDPGPRTCSAAGCEITYQTLINEGLLPV
                     GYTGTNAQKASYKIVLKRSGTAPDYVINGLITTTSPWTEGKNIRYDLLGKAVQSAGVD
                     GGMSRNTKIASGYGGQWSENSNSYSNITGGGLLAYRVGYNSSMYSVYLRRDGTLPMTG
                     DLNLGGKSIKNIKDMTASGTTTTGALKTTGNASVASDLTVGGTSTLNGPVNINNNLKV
                     KSDTYLNTLSTTGLAKFGSRIATNGLNPNDLPAGWAGGVRTYDLYASGTVGVGTGKTV
                     KAYMNNAGNIYASGNLTAGTIKSNGTIESTGRIKAGEYLHLNGQATLNAKCTPNGLVG
                     RDSSGRVLSCVSGKWQTASGDGLKGIFITITDQTSGYKCVIPNSDTGACACPGSTYSP
                     QFGYISGTLIAEYDERRCSGSKNEHCYSNFRRLYACK"
     gene            complement(12143..12700)
                     /locus_tag="ACHOS5_004152"
     CDS             complement(12143..12700)
                     /locus_tag="ACHOS5_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFP9778880.1"
                     /translation="MVLLNNKRKSKKGFSLLELLLVLGIIAALVVAAFIVYPKVQASQ
                     RAQAESNNIATIQAGVKALYTSASSFTGLTNTVAVQAKIFPDNMLSGSGTAAKPINAF
                     KGNVTLAAAATGPSSAAGSSFTITYDNVPAAECVKITTAAAGNFYTAKVGSKVVKASD
                     GTLDVAATAAACNNATSNTLVFTSI"
BASE COUNT         3580 a         2894 c         2670 g         3591 t
ORIGIN      
        1 atgctggtcc cggcgttatt agttgctggc gcagcaaatg cggctgaaat ttataataaa
       61 aatggcaata aagttgagct atacggcaag atggttggtg agcgcattct gaccgaccgt
      121 gaaaatggtg aaaaaggcga taattctcaa gatacctctt atgcccgtgt aggcgtgaaa
      181 ggtgaaactc agatcaaccc tgaactgact ggttacggtc agtttgaact agacctggaa
      241 gcatcaaaca gacacaaccc tgaccaaact cgtttggcat atgctggttt gagttacaaa
      301 gattttggtt cattcgacta cggtcgtaat gtcggtgtcg cgtatgatgc agaagcattt
      361 actgatatgt ttgttgagtg gggcggtgac tcatgggctg gtactgacct gttcatgacc
      421 aaccgtacca acggtgttgc aacctatcgt aacacagatt tctttggcat ggttgaaggt
      481 ctaaattttg ctctgcaata tcagggtaaa aatgaaggta caggcaacta caaagcaaat
      541 ggcgatggcc atggtttatc tgcaacttac actattgacg gtttctcatt tgctggtgct
      601 tatgcaaact ctgaccgtac tgattggcag tctggtgatg gtaaaggcga aagagccgaa
      661 gtttgggcgt tgtcaacaaa atatgacgct aacaatgtct atgctgctgt gatgtacggt
      721 gaatctcaca acatgaattc cgacgacggt gatgttgtga acaaaacaca aaacttcgaa
      781 gctgttctgc aatatcagtt cgactttggt ctgcgtccat ccatcggtta ctcctattct
      841 aaagcactgg acgtagcagg ttacaaagat tctgatcgtc tgaactatat tgaaatcggt
      901 acctggtatt acttcaataa gaatatgaac gtatataccg cttatcagat caacctgctt
      961 gataagagtg attatgttct cgctcatgga ctgaacaccg acgaccaact ggcagttggt
     1021 atcgtctacc agttctaatc agccaacgcg ccgaggcgtg ttactgtaaa acataacacc
     1081 agcctgagtt cgtccctcat cgaactcagg ctttttttat tggcatttca tctccatctg
     1141 cggcacacgc aattgccaaa gcatctcgat acagccactt accctgcccc tcctggcaca
     1201 atatcctcta ccagcaactc ttccagccaa caccccaaat gcatttcttt ataaatttaa
     1261 tctgtaacaa aaccgcttcc catatagaaa tatttgcaaa aatagtgcat gaaaaaagct
     1321 ttttccctac caatccggga tataaatatt catcagtacc acaacattcc acagcactaa
     1381 atctctcccc atcgccccca aacctctgat aatggtctaa aatcattgag gccacttgcg
     1441 acgacgttac tcaataagga aagtttggcg tgaataatat tcaacaccag tgcagcatca
     1501 tcattacata cttatgcccg ccaactaccg ctttcgatat attgactcaa tttttcagcc
     1561 tggttaaaaa tagcgaccag ctcagtcgca aatatagtga ctaccctaac taagcaacaa
     1621 taaggaatac actatgactg ttcaaacaag taaaaacccg caggtcgata ttgctgaaga
     1681 taatgcgttc ttcccttcag aatattcgct tagccaatat acgagtcccg tctctgatct
     1741 tgatggcgtg gactatccaa aaccgtatcg cggtaaacat aaaattctgg tgatcgccgc
     1801 ggacgaacgt tatttgccga ccgataacgg aaaactgttc tcgaccggta accatccgat
     1861 tgaaacgttg ctgccgttgt atcatctcca tgctgcaggt ttcgaattcg aagtggcgac
     1921 catttccggt ctgatgacca agtttgaata ctgggctatg ccgcacaaag atgaaaaagt
     1981 aatgccattc tttgaacagc ataaatcgtt attccgtaat ccgaagaaac tcgcggatgt
     2041 tgtcgccagc ctcaacgctg atagcgaata tgcagcaatc tttgttcctg gtgggcatgg
     2101 cgcacttatt ggtttaccag aaagccagga cgtggctgcc gctttacagt gggcaatcaa
     2161 aaatgaccgt tttgttatct ccctttgcca cggtccggcg gcttttctgg cacttcgcca
     2221 cagcgataac ccactgaatg gttattctat ttgcgcattc ccagacgccg cagacaaaca
     2281 aacgccagat attggctata tgccgggtca tctcacctgg tacttcggcg aagagttgaa
     2341 gaaaatgggc atgaacatca ttaatgacga catcaccggg cgagtacata aggaccgtaa
     2401 acttctcacc ggcgacagtc cttttgcagc gaatgcgttg ggtaaactgg cggcgcagga
     2461 aatgctggca gcttacgcga gttaatccct gttacattgt gatgtaagcg cataatcaat
     2521 cgcagccacg ttaatactca cgacgtggct gcgttttttc acggttaact tatggcgtac
     2581 tgaagcccga tgttaatttc tttgcggtaa cgtaattcgg aacacgttat gcttactgag
     2641 atagtgatac gtagcacttc ctccatgtaa ttcggcaatc gctttgacta aagaaaggcc
     2701 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
     2761 ttcaggctca ttaattttcg ttccaggact ggcgatatca atattaagag aaccgttggc
     2821 atcaagaaaa ctggttatat gaatacgcga tttttctggc gaatatcgaa tggcgttaac
     2881 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gttgattgca
     2941 ctcgacctta aaacgaatct ctttctcgtc tgaaagatat tcaagatagt ccaaaagatt
     3001 ttcaacttcc ttgttgagag aaagcgcatt cagtttcacc aaaacattgt ttttgtctgc
     3061 cctggcaaga aacagtatat tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
     3121 tccggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
     3181 ctgacccagt aatgcattaa ttggcgttct aagttcatga gcgagatcgt cagcaaattg
     3241 actaaggcgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
     3301 aggttttagt tctctcggta atgcgttaac ctcaaccggc actcggctat cgttataatt
     3361 cagcgcttcc gttacgccac tcaacttttt gatctctcgc aaccccgttc tgattaacca
     3421 cggacttaat aatgagcaca atataatgac gacaatgcaa attataatgc tattaatttt
     3481 atactgttca agcatattat gtctggctga agccaattta gccacagtaa ccgttaatga
     3541 tggcgaaact tcatcaattt taattcgtaa agcatctatc tctgtatcat taataatgct
     3601 tctgtaaatg cctgctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     3661 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     3721 ctgactaaca tccatcatcc ggttaaagta cacaggtaat gtatctggat ttaccccatc
     3781 aattaacaac tgcttgatct gcgctgtccg gttaatgagt gttgtatcat cgcgccattt
     3841 caactcactt gccaggccat tatagagagt ccatacaatt ccagcgccag caacagacag
     3901 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     3961 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     4021 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     4081 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatctc gccagctctg
     4141 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc
     4201 ctgctcacac tttgactgac agagtccatt tttaagccac tgatttctaa tgttgaattc
     4261 agagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     4321 ggttttacca gataatcatt tgccccactg tccagccctc tgactctgtc atcgacagaa
     4381 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     4441 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     4501 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     4561 gaaagcccct gcgttaccca ttcctgggtc ctttgattat cttcaataag tagaatcttc
     4621 atgaaaataa tatgccataa atcatatgtt atagaaacaa cctggttcat tacaaaattg
     4681 taatgctgct gtaaggttac cctggacgtt ttttcgctat cctcaaaact cattcacatg
     4741 acaaggatat aaacatgtta aagcgttatt tagtactctc ggtagcaacg gcagcatttt
     4801 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     4861 agcaaacagg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggataacg
     4921 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     4981 ccgagcaaac tgcgactact ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     5041 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     5101 tgaacgagca ttatcatgtg cctttgttac tcagccaata tgggtattca acctatcgtg
     5161 gcagttaatg cgtaaatatg taaaagctga tctctgctac tgttagggag gtgactccct
     5221 gacagattaa cagtaaacgg ctcttgttgg ctaacgacaa aaaagtgtga tggcttatga
     5281 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     5341 gtcggcaggt gttaaaagca ctgggcatca gcgcagctgc attttctttg cctcacgctg
     5401 cgcatgccga tctgcttagc tggtttaaag gaaacgatcg tccgcccgcc cccgccggaa
     5461 aaccgctgga gttcagcaag cctgccgcct ggcaaaataa cctgccactg acgccagcag
     5521 ataaagtctc tggttataac aacttctatg aattcgggct ggataaagcc gatcccgctg
     5581 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     5641 aaccattgac cctcgatcat gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     5701 atcgtatgcg ctgcgtggaa gcgtggtcga tggtggtgcc gtggattggt tttccgctac
     5761 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacga
     5821 tttatgcgcc ggaacagatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     5881 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     5941 taggtgttta tggcaaggcg ttaccaccgc aaaatggcgc gccggtgcga ctgattgtgc
     6001 cgtggaaata tggctttaaa gggattaaat cgatcgtcag tattaagctg acccgcgagc
     6061 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     6121 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     6181 gcattctcga tgttcagcgc cagccaacac tactgtttaa tggttacgcc gaccaggtgg
     6241 catcgctgta tcgtggcctg gatttgcggg agaatttcta aatgcgtctg acagcaaaac
     6301 aggtgatatg gctgaaagtt tgcctgcatc ttgccggatt gttgccgttt ctctggctgg
     6361 tctgggcgat caatcacggt gaactgggtg ccgatccggt gaaagatatt cagcatttta
     6421 ccggtcgcac tgcactgaaa tttttgcttg cggccttgtt aatcacacct ctggcacgct
     6481 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     6541 gggcgacact gcatttaacc agttacgcat tgctggagtt gggcgtgaat aatctggcgt
     6601 tattaggtaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     6661 ttttgcttgc tttagcgttc acttcaaccc agtcgatgca gcgaaaacta ggcaaacact
     6721 ggcaacagtt gcataacttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     6781 ggtcagtgaa gattatttca ccgcagccac tcatctacgc tgggctggca gtactgcttt
     6841 tagccttacg gtataagaag ttgctttccc ttttcaaccg gttacgcaag caggcccaca
     6901 acaaattatc tctataactt catattgccg acaaacttcg cgttatcccg gccggatgcg
     6961 gcgcgagcgc cctatccggc ccacacagat cgcatcaagt tatgtttcgt ttgtcatcaa
     7021 cctttcatca ccagaataat ttattttatt tcaataagat agttcaatac acctaagaat
     7081 accatgctct catttcctaa tgagttgttg cattttttca tatgttacaa tataacatta
     7141 cacatcatgt acattaactc tggaggaaat tgttttggcg attcgtcttc ataaactggc
     7201 tgttgcttta ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcactcaca
     7261 cggcaaaccc ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgataccaa
     7321 tgtacaaaac cgaacgctca gtgactggga tggagtctgg caatccgttt accctttact
     7381 gcaaagtggc aaacttgatc ccgtctttca gaagaaagcg gatgcagata aaaccaaaac
     7441 atttgctgaa attaaagatt attatcgcaa aggttatgcg acagatatcg aaatgattgg
     7501 cattgaggac ggcattgtcg aattccatag aaataatgaa acaacatcct gtaaatatga
     7561 ttacgatgga tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt
     7621 cgaatgtaaa gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat
     7681 tgcaccacga aaatcatctc attttcacat ttttatgggt aatgattccc agcaatcttt
     7741 attgaatgaa atggaaaact ggccaacgta ttatccctat cagttgagta gcgaagaagt
     7801 ggtcgaggag atgatgtctc attgattttt ttgcgctgac ttcagttatt taacagaaca
     7861 gtgcctggta gtaacaacaa taatttacca agacaaataa gtaagaaaaa ctcagacacg
     7921 ctcatacttg agtcgtgtct tttttattat gtctgtagaa aaaaactgag aaaaaggcga
     7981 acaataaata cacaactcag ccatacagat tggcagcgca aatgtctcca ggagcaagtc
     8041 atctgtcatg gtaaagctaa ccagaaatat aggttaattt tggtgtttaa acctttttta
     8101 cttgttagta tgatacaaag gctttcaaaa aagctgggaa attcaacaac tcttggaaat
     8161 tatggacttt gtcccgcggc tttaatctga cggccgcgtt ccttttttgg ctcaattatt
     8221 gaccccttct ctacgcaact tcagttcccg ccagcaactt tgcggcagtt tcataggatc
     8281 aatgtctaaa agaataatgg tgaccgataa gaaaacgact gaataactgt agattttcgc
     8341 tcgaaacctt cctgtcagct ccatagcgaa tcaagtgctg aatgtcacag tatcgaacag
     8401 aaaacagtga cgatctaacc cttcaagaat attctacgat tgttctgttt aggaaaagca
     8461 aggcgggaag tcgggagata agtcattgat aaagtggcgg agagagggtc taaattttta
     8521 gtgttcgaca atgcccacca aagtgctatt tttcctttaa aaacaaaata ttgtcattat
     8581 ttttttgttc ggtagtgtat tgctactgtg caaacaatca gctattttat gcagtaccgt
     8641 atgtatggcg ggttgtatga caggattttg ctatgccaaa gaaggccaag gaactctcag
     8701 gactcgtcgt atcacgatta aaatccgaag gcatgtatgc cgttggtggt gtggatggtt
     8761 tataccttcg catccgaaat cagtcccgtg catgggttct ttgtgtggct atgggaaccc
     8821 gaatcaacaa tctcggtaga atagttccaa gacgtttgaa tatggggctt ggcccctacc
     8881 cagaagtctc cttagccgaa gcgcgtgaca aagcgcgtga gctacgtaaa aaaatccgta
     8941 atggtattaa tccccttcag gaaaaacacg agcaaaaagc ccggcaggaa atactggccc
     9001 ggaagaaaaa gacctttgcc gaatgttgtg aagaagtgct ggaagtcaaa gacagtgaga
     9061 tgaagaacaa aaaacatctc gcgcaatggc gttccacact ggaaacctat gcctaccctt
     9121 tcattggcaa aaaggccgtt agtgaaatca ccaaagtgga tcttctggca atactcgaac
     9181 ctatctggtt aaccaagaat gaaactgcca gtcgtctacg tggacgtatt gaaacagtga
     9241 tcgattacgc caaggccaaa gaatactttg aaggtgataa tcctgcagca tggaaaggta
     9301 tgctgaaacc tctgttgcct caaccaagta aggttcagat caccaaacac catgcggccc
     9361 ttccctataa ccagctcggc tcctttatga aagaactacg tgaacgaagc ggcgtttctc
     9421 ctcgtgcgct tgagtttgcc atactgaccg cagcacgctc aggtgaaatt cgtggtgccg
     9481 agtggtcaga gatcgatctt gaaggtaaaa catggaccat ccccgccagt cgaatgaaag
     9541 cagcgaagga gcatcgagtt ccgttgtctg atgctgctgt tgccctgtta aaggctttac
     9601 cacgctttaa aggtatcaat tttgtattcc ctgccactcg gaagggacaa ctctcggata
     9661 ctgctttact ggcagtccta aagcgaatgg gatataccga cttaacgcag catgggttta
     9721 gatcgacatt ccgtgattgg gctggtgaaa cgaccaacta cccgcgtgag gtaattgaac
     9781 atgcgttagc tcatcagttg gcaaataagg cagaagctgc gtatcaacgt gggacgttat
     9841 ggcctaagcg ggtggcgtta atggatgatt gggcggggta ttgtgtttct tagaagttct
     9901 aattagctac aaatagtccc cgccctttat gaactgacac cataaattct taatgattgt
     9961 ataagattta tcaatttaga gtaatatacg acagaaacgt ttcacatgaa tatgctgttt
    10021 ttaaggagaa aaatgaaatg agtaaactgg actggttcaa taacgtagaa aatcgcccac
    10081 atccttggga aggtatgggg ctgacatggt tgacaacagc actaaatgtc cctgatgtaa
    10141 aagcagctat taatttttac actgaagtaa tgaagatggt ggtaatttct gaacttgagg
    10201 gtgatggtgg tgatgtatta tttgctcgta tccgttaccg tggcacaaac ttcaccataa
    10261 ataaggaagg atgggattcc gacctgaaaa gcccaaatac ttcgggagct atgcctccct
    10321 ttatttttta tatgtatgta gatgatatga tcactctggt aaatgaaatg gttgaagctg
    10381 gctcgactgt tctaaatgaa cctgctgaaa tgttctgggg tgatttaaaa gcccgtgtga
    10441 aagacccatt tggttttatc tgggatatag cacagaaact gtaaagaaat cgtcagatca
    10501 tcgttcaact cctcttttcg gttaaagtag gctgttgaaa tcaaaatgca caacacattt
    10561 aattcttact atgtcgtgca tttttgattc aaataattaa tacaaaatca attctgtata
    10621 ctacttttat ttacatgcat ataaccttct gaaattacta taacagtgct cgttcttact
    10681 accactacaa cgacgttcat catattccgc aataagtgtt ccagatatat aaccaaactg
    10741 aggactatac gttgatcccg ggcaggcaca tgcacctgta tcactattcg gtataacaca
    10801 tttatatcca gatgtttgat cggttattgt tataaaaata cctttcaggc catcaccgct
    10861 tgctgtctgc catttaccac tgacacacga taacactcga cctgaactgt ctcgccccac
    10921 cagaccatta ggcgtacatt tcgcatttaa tgttgcctga ccatttaaat ggagatattc
    10981 acctgcttta attctgcctg ttgattcaat tgtcccgttg gattttatcg ttccggcggt
    11041 aagattacct gacgcataaa tattccccgc attgttcatg taagctttaa ctgttttacc
    11101 ggttcctaca ccaacagtcc cagacgcata caaatcatag gtgcgaacac cgccagccca
    11161 gccagcaggc agatcgtttg gattaagtcc attcgtcgca atacggctac caaacttcgc
    11221 cagacctgtt gttgaaagcg tattcagata ggtatcggat tttaccttca ggttgttgtt
    11281 gatatttaca ggaccattaa gggtcgaggt tccacccaca gtcagatctg atgcaacaga
    11341 cgcattaccc gttgtcttaa gtgctccggt tgttgttgtt cctgatgccg tcatatcttt
    11401 gatgtttttg atgctcttcc caccaagatt cagatcaccc gtcataggca acgttccatc
    11461 acgacgcaga taaacagaat acattgaaga gttatacccc acgcggtaag ccagtaaccc
    11521 accacccgtg atattgctgt agctattaga attctcactc cactgaccac catagccgga
    11581 cgctatcttg gtgtttctgc tcatcccacc atcaacacct gccgactgaa ccgcttttcc
    11641 taacaggtca tagcgaatgt tctttccttc tgtccacgga ctggttgttg ttataaggcc
    11701 attaatgacg taatccggcg ctgttcctga acgctttaac acaattttgt aggatgcttt
    11761 ttgcgcattc gttcctgtat agcctacagg caacagacct tcattaatta acgtctggta
    11821 agtgatttca caaccagcag ccgaacaggt tcttggcccc ggatcactgc tttgattgtt
    11881 ggatgatgac agtgtggaaa tcttgtcata gcgaatactg atatagcggt taactgcttc
    11941 accaagctgt ttaatctgtg ttccaacatt atcagccaat actgcttcct gatcgtttct
    12001 catatcctga aacttcataa aagcgataag cgttccaata cctaacacaa tagtcacttc
    12061 cagtaaggta aatccttttt tctttatcat aattatctcc ctgttttagc aataagaatg
    12121 aaaagggcct gcgccctctt cattaaatag acgtgaaaac taatgtatta cttgtggcgt
    12181 tattacaggc tgctgccgta gccgccacat caagtgttcc atccgaagcc ttcacaacct
    12241 ttgaacccac tttagcggta taaaagttgc ctgctgccgc agtggttatt ttcacgcatt
    12301 ccgctgctgg cacattatcg tatgtgatag tgaaagaaga gccagccgct gatgacgggc
    12361 cagttgctgc cgcagccagc gtcacattac ctttaaaggc attgatgggt ttagctgccg
    12421 ttcctgaccc acttaacata ttgtcaggga agatttttgc ctgaacagcc acagtatttg
    12481 tcaggccagt aaagcttgat gccgacgtgt ataacgcttt aacacctgcc tgtatggtgg
    12541 caatgttatt ggattctgcc tgcgctcttt gtgaagcctg aaccttggga taaacgataa
    12601 aagccgctac cactaatgca gcaatgatgc ctaaaacaag taatagttcg agtagtgaga
    12661 aacctttctt gctttttctt ttgttattta ataaaaccat tattatctcc ttttttgttt
    12721 taatgatatc tcctg
//
LOCUS       DAZKHK010000137        12728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKHK010000137 DAZKHK010000000
VERSION     DAZKHK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.138"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            202..1716
                     /gene="caiT"
                     /locus_tag="ACHOS5_004153"
     CDS             202..1716
                     /gene="caiT"
                     /locus_tag="ACHOS5_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP9778881.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1747..2889
                     /gene="caiA"
                     /locus_tag="ACHOS5_004154"
     CDS             1747..2889
                     /gene="caiA"
                     /locus_tag="ACHOS5_004154"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP9778882.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3017..4234
                     /gene="caiB"
                     /locus_tag="ACHOS5_004155"
     CDS             3017..4234
                     /gene="caiB"
                     /locus_tag="ACHOS5_004155"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP9778883.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4307..5860
                     /gene="caiC"
                     /locus_tag="ACHOS5_004156"
     CDS             4307..5860
                     /gene="caiC"
                     /locus_tag="ACHOS5_004156"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP9778884.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPLSANDQQHRLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5969..6754
                     /gene="caiD"
                     /locus_tag="ACHOS5_004157"
     CDS             5969..6754
                     /gene="caiD"
                     /locus_tag="ACHOS5_004157"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP9778885.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6760..7350
                     /gene="caiE"
                     /locus_tag="ACHOS5_004158"
     CDS             6760..7350
                     /gene="caiE"
                     /locus_tag="ACHOS5_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP9778886.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7469..7864)
                     /gene="caiF"
                     /locus_tag="ACHOS5_004159"
     CDS             complement(7469..7864)
                     /gene="caiF"
                     /locus_tag="ACHOS5_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP9778887.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8125..11346)
                     /gene="carB"
                     /locus_tag="ACHOS5_004160"
     CDS             complement(8125..11346)
                     /gene="carB"
                     /locus_tag="ACHOS5_004160"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9778888.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEVVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11364..12512)
                     /gene="carA"
                     /locus_tag="ACHOS5_004161"
     CDS             complement(11364..12512)
                     /gene="carA"
                     /locus_tag="ACHOS5_004161"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP9778889.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         3004 a         3311 c         3355 g         3058 t
ORIGIN      
        1 aatcaaaatt aacaaaaaaa tgccctctat tcacccgaaa caaaaatgtg ataacaatca
       61 cagaatgcag cttattgaat acccattatg agttagccat taacgcgtcc acgaggttaa
      121 taataattat attaaatgtt aacaaaaata aaacaaacgg gaacgcaaaa taaatattcg
      181 ttttcacagt ggaattaact catgaagaat gaaaagagaa aaacgggaat agaaccgaag
      241 gttttctttc cgccgttaat aatcgtcggc atactttgtt ggcttacagt cagagatctg
      301 gatgcggcga atgtcgttat taatgctgta ttcagttacg tcaccaatgt atggggatgg
      361 gcatttgaat ggtatatggt ggtgatgctt ttcggctggt tctggctggt gtttggcccg
      421 tatgccaaaa agcgtttagg taacgaacca ccagaattta gtaccgccag ttggatcttt
      481 atgatgttcg cctcctgtac gtctgctgcc gtactgttct ggggatcgat tgagatctac
      541 tactacatat ccaccccgcc gtttggctta gaaccgaact caacaggcgc gaaagagttg
      601 gggctggctt acagcttgtt ccactgggga cctctgccgt gggccactta cagcttcctt
      661 tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg aagtgattcg ccccagctcc
      721 accctggtgc cgctggtagg tgaaaaacac gccaaagggt tgttcggcac tatcgtcgac
      781 aacttctatc tcgtcgcctt gatcttcgcg atgggtacca gtctgggcct tgccacgccg
      841 ctggtgaccg agtgtatgca atggttgttt ggcattccgc ataccctgca actggacgct
      901 atcatcatta cctgctggat tatcctcaac gccatttgcg tcgcttgcgg tctgcaaaaa
      961 ggggtacgta tcgccagtga cgtgcgtagt tacctgagct tcctgatgct gggttgggtg
     1021 ttcatcgtca gcggtgccag cttcatcatg aactacttca ccgattcggt ggggatgttg
     1081 ctgatgtatc tgccgcgcat gttgttctat accgatccca tcgctaaagg cggcttcccg
     1141 cagggctgga ccgtgttcta ctgggcatgg tgggtgattt acgctatcca gatgagtatc
     1201 ttcctcgccc gcatctcccg tggtcgtacc gtgcgtgaac tgtgcttcgg catggtgctg
     1261 ggactgacag cgtcaacctg gatcctgtgg actgtactcg gtagtaacac tctgctgttg
     1321 atagataaaa acattatcaa cattccaaat ctgatcgaac agtacggtgt ggcgcgcgcc
     1381 atcatcgaaa cctgggccgc tctgccactc agcaccgcca ccatgtgggg cttcttcatc
     1441 ctctgcttta ttgccaccgt tacgctggtt aacgcctgct cttataccct ggcgatgtcc
     1501 acttgccgcg aagtgcgtga cggcgaagaa cccccgctgc tggtgcgcat tggttggtca
     1561 gttctggttg gcattatcgg cattgttctg ctggcgctcg gcggcctgaa accgattcaa
     1621 accgccatta tcgccggagg atgcccgctg ttcttcgtca acatcatggt gacgctctcc
     1681 tttattaaag acgcgaaaca gaactggaaa gattaattaa cccccaaaat atcaagaggt
     1741 tgaaagatgg attttaattt aaatgatgag caggaactgt ttgtcgccgg tatccgcgaa
     1801 ctgatggcca gcgaaaactg ggaagcctat tttgccgagt gcgaccgtga cagcgtctac
     1861 ccggaacgtt ttgtcaaagc cctggcggat atgggtatcg acagtctgct gatcccggaa
     1921 gagcacggtg gtctggacgc ggggtttgtt actctcgccg ccgtgtggat ggaactgggt
     1981 cgtctgggag cgccaactta cgtgctgtat cagttaccgg gcgggtttaa caccttcctg
     2041 cgtgaaggta cgcaagagca gatcgacaag atcatggctt tccgcggcac cggtaagcag
     2101 atgtggaact cagcaattac cgaaccgggc gcaggctccg acgtgggtag cctgaaaacg
     2161 acttataccc gtagaaatgg taagatttat cttaatggta gtaagtgttt tattaccagt
     2221 agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg gggcttctcc ggacaaacct
     2281 gtctacaccg aatggtttgt tgatatgagc aaaccgggca tcaaagtgac caaacttgaa
     2341 aagctcggtc tgcgtatgga tagctgctgt gaaatcactt ttgacgacgt ggaactggac
     2401 gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc gcgtcaaaga agagttcgac
     2461 catgaacgtt tcctggtagc cctgaccaac tacggtacgg cgatgtgcgc ctttgaagat
     2521 gcggcgcgct acgccaacca gcgcgtgcag tttggcgagg ctattggtcg tttccagttg
     2581 attcaggaaa aattcgccca catggcgatc aaattaaact ccatgaaaaa catgctgtat
     2641 gaagcagcgt ggaaagcaga caacggcacc atcacctctg gcgatgcagc gatgtgcaaa
     2701 tacttctgcg ccaatgcggc atttgaagtg gtggatagcg caatgcaggt gctgggcggt
     2761 gtcgggattg cgggcaacca ccgcatcagc cgcttctggc gtgacctgcg tgtagaccgc
     2821 gtttccggag gatctgacga aatgcagatc ctgacgctgg gtcgcgcggt gctgaagcaa
     2881 taccgttaag tccgtgcgct gcctgatgcg acgctgacgc gtcttatcag gcctacggga
     2941 gcacgaagat ggccggataa ggcgtttacg ccgcatccgg cagtcatgcg ctacataaat
     3001 ttttcaggag ctaataatgg atcatctacc catgccgaaa ttcgggccgc tggccggatt
     3061 acgcgttgtc ttctccggta tcgaaatcgc cgggccgttc gccggacaaa tgttcgcaga
     3121 atggggggcg gaagttatct ggatcgagaa cgtcgcctgg gccgacacca ttcgcgttca
     3181 accgaactac ccgcagctct cccgccgcaa tttgcatgcg ctgtcgttaa atattttcaa
     3241 agatgaaggg cgcgaagcgt ttctgaaatt aatggaaacc accgatatct tcatcgaagc
     3301 cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtcctgt ggcagcacaa
     3361 cccaaaactg gttatcgctc acctttccgg ttttggtcag tatggcaccg aggagtacac
     3421 caatcttccg gcctataaca ctatcgccca ggccttcagt ggttacctga ttcagaacgg
     3481 tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt ctggcctgac
     3541 cgccaccacg gcggcgcttg cagcactgca taaagtgcgt gaaaccggca aaggcgaaag
     3601 catcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca tgatggatta
     3661 cttcaacggc ggcgaaatgt gcccgcgcat gaccaaaggt aaagatccct actacgccgg
     3721 ttgcggtctg tataaatgcg ccgacggcta catcgtgatg gaactggtgg gcattactca
     3781 aattgcagag tgctttaaag atattggcct cgcacacctg ctcggtacgc cggaaatccc
     3841 ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg tagaagagaa
     3901 actcgatgcc tggctggcgg cacataccat cgcagaagtt aaagaacgct ttgccgaact
     3961 gaatatcgcc tgtgccaaag tactgaccgt gccggaactg gaaagcaatc cacagtatgt
     4021 ggctcgcgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca aagggccgaa
     4081 tatcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc cctcacatgg
     4141 catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca ttcaggagtt
     4201 ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc aacttgcctg
     4261 actagcgccc gcccaataac aaacgaacaa aatggataga ggtgcaatgg atatcattgg
     4321 cggacaacat ctacgtcaaa tgtgggacga tctggcggac gtttacggtc ataaaacggc
     4381 gctgatttgt gaatccagca gcggagtcgt taaccggtat agttatcttg agttaaatca
     4441 ggagattaac cgcacggcaa acctgtttta tacgctgggg attcgcaaag gcgacaaggt
     4501 tgctctacat ctcgacaact gcccggaatt tatcttttgc tggttcgggt tggcaaaaat
     4561 tggcgcgatt atggtgccga ttaacgcccg cctgttgcgc gaagaaagca cgtggatcct
     4621 gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa ttttatccta tgtatcaaca
     4681 gattcagcag gaagatgcct ctcagttgcg gcacatttgc ctgacagata tggtacttcc
     4741 cgctgatgat ggcgtgagtt cgtttactca actgaaaaat caacaacctg ccaccttgtg
     4801 ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac
     4861 ctcccgaccg aaaggggtgg tgataactca ttacaacctg cgcttcgctg gatattactc
     4921 cgcctggcag tgtgcactgc gtgacgatga cgtctacctg acggtaatgc ctgcgtttca
     4981 tatcgattgc cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct
     5041 ggtcgagaaa tacagcgccc gcgccttctg gggacaggtg cagaagtacc gcgccaccat
     5101 taccgaatgt attccgatga tgattcgtac gttgatggtg cagccgcttt cagcgaacga
     5161 tcagcaacac cgcctgcggg aagtgatgtt ttatctcaac ctgtcggagc aggaaaaaga
     5221 cgcgttttgt gaacgcttca gcgttcgctt gctgacgtct tatgggatga cggaaaccat
     5281 tgtgggcatt atcggcgatc gccctggcga taaacggcgc tggccgtcga ttggtcgggc
     5341 gggcttttgc tacgaagcgg agatccgcga cgatcacaat cgtcccctcc cggctggtga
     5401 gctcggtgaa atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct
     5461 caacccgaaa gccactgcga aagtgctgga agccgatggc tggctacata ccggcgatac
     5521 cggataccgc gacgaagagg gcttttttta tttcgtcgat cgccgctgca atatgatcaa
     5581 acgcggtggc gagaatgtct cctgcgtgga gctcgaaaat atcatcgcca cccatccaaa
     5641 aattcaggat atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc
     5701 atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag gaattcttct gcttctgtga
     5761 acaaaatatg gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg
     5821 taattgctcg gggaaaataa ttagaaagaa tctgaaataa atgaaacggc agggaactac
     5881 cctgtcagct aataatcacg ctctaaaaca atatgcattt tttgcaggga tgttgtcgtc
     5941 cctgaaaaag caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg
     6001 atcaattctg gaaattaccc ttgatcgtcc aaaagcgaat gctattgatg caaaaaccag
     6061 ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat
     6121 tattaccggt gccggagaga aattcttttc cgcgggctgg gatttaaaag cggcagcaga
     6181 aggcgaagca ccggatgctg actttggccc gggaggtttt gcgggattaa ccgaaatttt
     6241 caatctcgac aaaccggtca tcgccgctgt gaacggctat gcctttggcg gcggctttga
     6301 actggcgctg gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctaccgga
     6361 agccaaactg ggcatcgttc ctgacagcgg cggcgtgctg cgtctgccga agatcctgcc
     6421 gcctgccatc gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct
     6481 gcgttggggg gtagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga
     6541 actggcgcag cagctggtta acagcgcacc actggcgatc gcagcgctga aagagatcta
     6601 ccgcaccacc agcgaaatgc cagtagaaga agcgtatcgc tatattcgca gcggcgtgtt
     6661 gaaacactat ccatcggttc tgcattcgga agatgccatt gaagggccac tggcgtttgc
     6721 cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgaa
     6781 gggttaattc cggttgttca cccgacggcg tttgtccatc ccagtgcagt attgattggc
     6841 gatgtgattg tgggagccgg tgtctacatc ggcccactcg cctcactgcg tggtgactac
     6901 gggcggttga tcgtgcaagc gggagccaat attcaggatg gctgcattat gcatggctac
     6961 tgcgacactg acaccatcgt tggggaaaac ggtcatatcg ggcacggagc gatcctgcat
     7021 ggttgtgtga ttggtcgcga tgcactggtc ggtatgaaca gcgtgattat ggatggcgcg
     7081 gtcattggcg aagagagcat tgttgccgct atgagctttg tcaaagcggg ctttcgcggc
     7141 gagaaacgcc agttgttgat gggtacgcca gcccgcgctg tacgcagtgt gagtgacgac
     7201 gagttacact ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat
     7261 gcatcgttac atgaaacgca gccactgagg caaatggagg aaaatcgccc ccgtttgcag
     7321 gggacgacgg atgtgacgcc gaaacggtaa catcgtcgga tgcggcataa acgccttatc
     7381 cgacctggct gtaaagcaac cgtaggccgg ataagatgcg ccagcatcgc atccagcgac
     7441 caggtcgcac ggtatcaacg aaatgacatt aacgacgcat actctttgac aacatcatct
     7501 gccacttctg ctcgcggtta agttccgtga gcggaacggc aggaatacgc ggcgtctttc
     7561 ttacgccgac taatttatcc cggctgttat tgcgcaggcg cgcgtaaatt tgctcatcga
     7621 tatcaaccac ttttaccagt cgctggcact ggcatccccg cccttccagc ttattaggga
     7681 tcatcttaac ttcgcagctt atttctgtga cttccgacag aatataagtc agggtattaa
     7741 ccgccttgct gtgttcaata tcgaaatgaa tagagatctc tcttgctatc acccaccggt
     7801 tttcagccat catccattcg gcgattaaca aatagagtgg tttttcaaca catccttcac
     7861 acatacgaac tccagtttca caaattaaaa gactggctat gactcgtttt gcagcaatta
     7921 aacaaggcca tattaaaatt gtggatatat cgatagtatg caacttcacc gtcgataagt
     7981 gtgagctaaa tcctgcttat gtcatcaaaa atatcgattc cccaaaagat ttacttaatg
     8041 ggcaaaaaaa acggtcctca tcagagaacc gtattagtta ttcgatcaaa ttagcggatg
     8101 aaaaatatcc gccatgacac gctattattt gatctgcgcg tgcatttcct gcaccgaaat
     8161 tactttttcg gtcgcatcgg cattcagcgc catcgcggtg gcaaagccgc cgttcagggt
     8221 ggtgtcgtaa tgcactttat attgcagcgc actgcgacgg atcacgcggg agtcttcaat
     8281 cgcacgacga cctgaggtgg tgttgatgat gtaggtatat tcgccattct taatacggtc
     8341 ctgaatgtgc gggcggcctt catgcacctt gttcaccaga cgcggattga tacccgcttc
     8401 gcccagcaca atcgccgtgc cgtgagtcgc atccagctcg aagccctgtt tcagcagttt
     8461 tgccgccagg tccaccacgc gttctttatc gccttcgcgc acggaaagca gcgcacgacc
     8521 gtgtttcttc atggtggagt tgctgcccag ctgcgctttg gcaaacgctt cagcgaaggt
     8581 gcggcccacg cccattactt ccccggtaga gcgcatttct ggccctaaca gcgggtcaac
     8641 gcccgggaat ttattgaacg gcagcaccac ttctttcacc gagtagtacg gcgggataac
     8701 ttctttggtt acgccctgct cagccagcga tttgccagcc atcacacgcg ccgccacttt
     8761 tgccagcggt acgccggtgg ctttggagac gaacggaacg gtacgcgccg cacgcgggtt
     8821 aacttcaatc aggtagactt cgttgttttt caccgcaaac tgcacgttca tcaggccgcg
     8881 cacctgcaat tcgaaggcca gtttctgcac ctgctggcgc atcacatcct gaatttcctg
     8941 acttaaggtg taggctggca gcgaacatgc ggagtcaccg gagtgcacgc ccgcctgctc
     9001 aatatgctcc atgatgccgc caatcagcac cacttcgccg tcgcagatgg catccacgtc
     9061 aacttctact gcgtcatcaa ggaagtggtc cagcaacact ggcgcatcgt tagatacgct
     9121 gaccgctgtc tggaagtagc gacgcaggtc agcttcgtca tagacgattt ccatcgcccg
     9181 accgccgaga acgtaagacg gacgcaccac cagcgggtag ccaatctctt tcgctttctc
     9241 aaccgccatt tcgatggtgg taacggtggc gttcgccggt tgtttcagtt tcagacgctc
     9301 aaccgcatgc tggaagcgtt cacggtcttc cgcacggtcg atggcatccg ggctggtacc
     9361 gataaccggt acgccagcag cttccagcgc gcgcgccagt ttcagcgggg tctgaccgcc
     9421 gtactggacg ataacgcctt tcggcttctc aatacgcaca atttccagca cgtcttccag
     9481 agttaccggc tcgaagtaga gacggtcaga ggtgtcgtag tcggtggaga cggtttccgg
     9541 gttacagtta accataatgg tttcataacc atcttcgcgc agcgccagcg aggcgtgtac
     9601 acagcagtag tcgaattcga taccctgacc gatacggttc gggccgccgc cgagcaccat
     9661 gattttttca cgatcggtgg acggattcgc ttcgcactct tcttcataag tggagtacat
     9721 gtaagcggtg tcggtggcga actctgccgc acaggtatcc acgcgcttgt aaaccgggtg
     9781 cagatcatac tggtcgcgca acttgcggat ttccgcttcg cgcacgcccg ccagtttagc
     9841 cagacgcgca tcggcaaagc ctttgcgttt cagctggcgc aggaattcag cgttcaggcc
     9901 agtgatgccc acttctgcga ctttctcttc cagacgcacc agctcttcaa tctgtaccag
     9961 gaaccagcgg tcaatgttgg tcaaattaaa cacgccgtct acagacaggc ccgcgcggaa
    10021 ggcatcagcg atataccaga tacgctctgc gccagcatct ttcagttcgc ggcggatttt
    10081 agtcagcgct tccgggtcgt cgagactcac tttcggatcg aagccagttg cgcccacttc
    10141 caggccgcgc agcgcttttt gcagggattc ctgctgcgta cgaccaatcg ccatcacttc
    10201 gccaaccgat ttcatctgag tggtcagacg gtcgttagca ccggcgaatt tctcgaagtt
    10261 gaagcgagga attttagtaa ccacatagtc gatggacggc tcgaaggagg ccggagtacg
    10321 cccaccggtg atgtcgttca tcagctcgtc gagggtgtaa cccaccgcca gtttcgccgc
    10381 cactttggca atcgggaaac cagtggcttt cgacgccagc gcagaggagc gagacacgcg
    10441 cgggttcatt tcgataacaa tcaggcgacc gtttttcggg ttcactgcaa actggacgtt
    10501 ggaaccaccg gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt tacgcatgat
    10561 ttgatattct ttatcggtca gcgtttgggc tggcgcgaca gtgatggagt caccggtatg
    10621 gatgcccatc gcatcgaagt tttcgataga gcagacgatg atgcagttgt cgtttttatc
    10681 acgcaccact tccatctcgt actctttcca gccgatcagc gactcatcaa tcagcagctc
    10741 tttagtcgga gagagatcca gaccgcgggc gcaaatttct tcaaactctt cgcggttata
    10801 agcgataccg ccgccgctac cacccatggt aaaggatggg cgaataatgc acgggaagcc
    10861 aacttcagcg gcaaccgcca gcgcttcttc cattgaatga gcgataccgg aacgcgcagt
    10921 ttccagaccg attttcttca tcgctacgtc gaaacggcgg cggtcttcgg ctttatcaat
    10981 cgcatcggca gtggcaccaa tcatggtgac gccgaactct tccagcacgc cctgacgctc
    11041 aagctccagc gcacagttca gcgccgtctg cccccccatg gttggcagca ccgcgtccgg
    11101 gcgctctttt tcaataattt tgcgtaccac ttcccagtga atcggctcga tgtaggttgc
    11161 atcagccatt tccgggtcgg tcatgatggt cgctgggttg gagttcacca gaatgacgcg
    11221 gtagccctct tcacgcaggg ctttacacgc ttgcgcgcca gagtagtcaa actcacacgc
    11281 ctgaccgata acaatcgggc ccgcacccag aatcaggata ctttttatat ctgtacgttt
    11341 tggcatggct cttttactcc tgattactta gcggttttac ggtactgctc aattaacgcg
    11401 ataaagtggt caaacaacgg cgcggcgtcg tgtggaccag ggctggcttc cgggtgtccc
    11461 tggaagctga acgccggttt atcggtgcga tgaatccctt gtaaagtacc gtcgaacagg
    11521 gatttatgtg tgacacgcag gtttgcaggt aatgttgctt cgtccaccgc aaaaccgtgg
    11581 ttctgggcgg tgatcatcac cacatttttc tcaacatctt taaccggatg gttgccgccg
    11641 tggtgaccaa atttcatttt gacagtcttc gcaccgctcg ccagcgccag cagctgatga
    11701 ccgaggcaga tgccgaatac cggaatatca gtttcgagga atttctggat agcggtaatg
    11761 gcgtaatcgc acggggccgg gtcgccagga ccgttggaga ggaagatgcc gtccggattc
    11821 attttcagca catcttccgc agaagtttgc gccggaacga tggtcaggcg acagcctcta
    11881 tccaccagca tacgcaggat gttgcgcttg gcaccgaaat cgtaggccac gacgtggtac
    11941 ggcagctcgt cttctttttt cgcttctggc aggccgccgg tcagtgtcca gctcccttgt
    12001 gtccagctat agggttctgc ggtggttact tcttttgcca gatccatgcc gttcagacct
    12061 gggaacgcgc gggctttttc taatgccagc gccgcatccg ggttatcgcc tgcgataatg
    12121 cagccattct gagcaccttt ctcacgcagt aaacgcgtca gcttacgggt atcgatatcg
    12181 gcaatcgcca cgatgttatg gcgcttcagg taagaagaga ggtcttcggt attacggaag
    12241 ttgctggcaa tcagcggcag gtcgcgaatc accagacctt gggcatgaac ctgagaagat
    12301 tcttcatcgg cgtcattagt gccgacattg ccaatatggg gataagtaag agtaacgatt
    12361 tgacgagaat aggaaggatc agtgaggatt tcttgataac cggtcattga agtattgaaa
    12421 acgacttccc caaccgccga acctgttgcc cctatggccc gaccgtgaaa ctgggttccg
    12481 tcttccagaa ccaatagcgc tgacttaatc aaaacaccct ccagagaata ttcactcact
    12541 ttatttgcat attaattcac aatgatgacg taaatcaatg caaatctgct taccgacgaa
    12601 tttctggcaa acggcggcat tctggagata tgggagctaa aagtcaactt aatggcggga
    12661 ttttttatat tattttgtgg ctcatgcatg ttttacaggc attaagcgac aaaaagatca
    12721 tataacat
//
LOCUS       DAZKHK010000138        12086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKHK010000138 DAZKHK010000000
VERSION     DAZKHK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.139"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..2224
                     /gene="iroN"
                     /locus_tag="ACHOS5_004162"
     CDS             47..2224
                     /gene="iroN"
                     /locus_tag="ACHOS5_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HFP9778890.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKYSDDDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSEEGATRRANFSLSGPLAGDALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDRDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKERLNWNTNATWMLTSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2269..3225)
                     /gene="iroE"
                     /locus_tag="ACHOS5_004163"
     CDS             complement(2269..3225)
                     /gene="iroE"
                     /locus_tag="ACHOS5_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HFP9778891.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSTTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKNKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3310..4539)
                     /gene="iroD"
                     /locus_tag="ACHOS5_004164"
     CDS             complement(3310..4539)
                     /gene="iroD"
                     /locus_tag="ACHOS5_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HFP9778892.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4643..8302)
                     /gene="iroC"
                     /locus_tag="ACHOS5_004165"
     CDS             complement(4643..8302)
                     /gene="iroC"
                     /locus_tag="ACHOS5_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HFP9778893.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGMASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSDISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAVAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSTIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVAQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLSAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            complement(8442..9557)
                     /gene="iroB"
                     /locus_tag="ACHOS5_004166"
     CDS             complement(8442..9557)
                     /gene="iroB"
                     /locus_tag="ACHOS5_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HFP9778894.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIRGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPDRKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            10095..10394
                     /locus_tag="ACHOS5_004167"
     CDS             10095..10394
                     /locus_tag="ACHOS5_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778895.1"
                     /translation="MKPTLLQTVENICLQILFVLFLFTGTLIDVEALVSCSSVAKNVQ
                     RTPASSVSSSVYSASVSRTAADIMLLSNNPLFAPGQVSFFGCSLAASGIHVTILE"
     gene            10572..11030
                     /gene="tnpA"
                     /locus_tag="ACHOS5_004168"
     CDS             10572..11030
                     /gene="tnpA"
                     /locus_tag="ACHOS5_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200C family
                     transposase"
                     /protein_id="HFP9778896.1"
                     /translation="MGNEKSLAYTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            11147..>12086
                     /gene="istA"
                     /locus_tag="ACHOS5_004169"
     CDS             11147..>12086
                     /gene="istA"
                     /locus_tag="ACHOS5_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFP9778897.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHEIIQARPCDRWLEEQQSMLALPPEKKEYDVHP"
BASE COUNT         2901 a         3202 c         3253 g         2730 t
ORIGIN      
        1 cttaacgatc aaacatttta agttaggcat ttattaggga ataggaatga gaattaacaa
       61 aatcctctgg tcgctaactg tgctcctagt tgggttgaat agccaggtat cagtagccaa
      121 atactccgac gatgataatg acgagactct ggtggtggaa gccaccgctg agcaggtatt
      181 aaaacagcag ccgggcgtgt cggttattac cagcgaggat attaaaaaga cccctccggt
      241 aaacgacctt tcagatatta ttcgtaaaat gcctggtgtt aatcttaccg gcaatagcgc
      301 ctcgggcaca cgcggtaata accgccagat cgatattcgt ggtatggggc cggaaaacac
      361 cttaatttta attgatggtg taccggtgac gtcacgtaac tccgtgcgtt atagctggcg
      421 tggggagcgt gatacccgcg gtgacaccaa ctgggtgcca ccggaacagg ttgagcgtat
      481 tgaagtgatc cgcggccctg cggcggcgcg ctacggttcg ggggccgccg ggggggtggt
      541 gaacatcatt accaaacgtc ccaccaacga ctggcacggt tcgctgtcgt tatacaccaa
      601 ccagccggaa agtagcgaag agggcgctac gcgtcgcgcc aatttcagcc ttagtgggcc
      661 tctggctggt gatgctctta ccacgcgttt gtatggtaac ctgaataaaa cggatgctga
      721 cagttgggat attaattctc cggtcggtac gaaaaacgca gccgggcatg aaggggtacg
      781 taacaaagat attaacggcg ttgtctcgtg gaaattaaat ccgcagcaga ttctcgattt
      841 cgaagccgga tatagccgcc aggggaatat ctatgcgggc gatacgcaga acagttcttc
      901 cagtgcagtt accgaaagcc tggcaaaatc cggcaaagag acgaaccgcc tgtaccgaca
      961 gaattatggc attacgcata atggtatctg ggactgggga caaagtcgct ttggtgttta
     1021 ttacgagaaa accaataata cccgcatgaa tgaaggatta tccggcggtg gtgaaggacg
     1081 tattttagcg ggtgaaaagt ttacgaccaa tcgcctgagt tcctggcgaa ccagcggtga
     1141 gcttaatatt cctttgaatg tgatggttga tcaaacgctg accgttggtg cagagtggaa
     1201 ccgcgataag ctcgatgatc cttcctctac cagcctgacg gtgaatgaca gagatatcag
     1261 cggtatttct ggctctgctg cggatcgcag cagtaaaaat cattctcaaa tcagtgcgct
     1321 gtatattgaa gataacattg agccggttcc tggcacgaat atcattcccg gcctgcgctt
     1381 tgattatctc agcgactccg gcgggaactt cagccccagt ctgaatcttt cgcaggaatt
     1441 gggcgattat ttcaaagtca aagcaggggt tgcccgaacc tttaaagccc caaacctgta
     1501 tcaatccagt gaaggctatc tgctctactc gaaaggcaat ggctgtccaa aagatattac
     1561 atcaggcggg tgctacctga tcggtaataa agatctcgat ccggaaatca gcgtcaataa
     1621 agaaattgga ctggagttca cctgggaaga ttaccacgca agtgtgacct acttccgcaa
     1681 tgattaccag aataagatcg tggccgggga taacgttatc gggcaaaccg cttcaggcgc
     1741 atatatcctc aagtggcaga atggcgggaa agctctggtg gacggtatcg aagccagtat
     1801 gtctttccca ctggtgaaag agcgtctgaa ctggaatacc aatgccacat ggatgctcac
     1861 ttcggagcaa aaagacaccg gtaatcctct gtcggtcatc ccgaaatata ctatcaataa
     1921 ctcgcttaac tggaccatca cccaggcgtt ttctgccagc gtcaactgga cgttatatgg
     1981 cagacaaaaa ccgcgtactc atgcggaaac ccgcagtgaa gatactggcg gtctgtcagg
     2041 taaagagctg ggcgcttatt cactggtggg gacgaacttc aattacgata ttaataaaaa
     2101 tctgcgtctt aatgtcggcg tcagtaatat cctcaataaa cagatcttcc gatcttctga
     2161 aggggcgaat acctataacg agccaggccg ggcttactat gccggagtta ccgcatcatt
     2221 ctgatcgtag tgtttcaggc gtactgcccg cgtaaaagcg ggcagtgttt agtggcttaa
     2281 ctcatgacaa cctgctgtgt aatttgcgtt ttcaccactg atatctaaca gtgcctggcg
     2341 aaaggaggca ttgaacatcg gcccgtgtcc caggttgggg aaatcccaaa atacggcatt
     2401 gacgccttta tttttcagta tagtgagggt ggtatgaatt ttcgacagca ccccgacagc
     2461 atgcgtttcc cggttatcac cctgtgtcgc cgagccttcc attatcgcca ggtgtttggc
     2521 gcagaattgc agaggctcaa ccgccgtaac gcggcttagc aaagcatcat aacctctgcc
     2581 caacgacggg ctggcgctgt agtacgaccg gaagtaagag gaggacagcc aggaatccag
     2641 cacgaagagg ccgccgtagg agtgccccca taagccgcgg cgttgccgat cgatattcag
     2701 tccctgttcc acttttgggg caatacgcgt ttccagtaac tggcggaagt tgttgctgcc
     2761 accactctta cggctaaaac gccctgagtg gagatctgtt tttctgcttt ctgctgctgg
     2821 cgtatagtcg taagccctgc tgttgagatc gaaagggagg ttggtctgat acccgacagc
     2881 cacgatcact ggcggtgttt tttctgacaa ttgtttgagc agttcgtcat ccaggcggtc
     2941 cataactgcg ttaccgtcaa gcatatataa aatcgggtaa cccgatgccg gtgcggttgt
     3001 attcggcacg gccgtccata cccgataatg gcgtgtgcca tcgacagagt cgaaagaggt
     3061 ggtgctgaaa tggtaaaaca cggagccttt atcggctata ttcggcccca gtggccgcat
     3121 atccggcttc gcataaactt gcgcactaca gataagggtg aggcaagaaa gatgaaagaa
     3181 aatcgcttta tgcgggcgtg ttgagcgata ctcgcgggca tacatctgat gattattcct
     3241 tttccgcagg tgacaatttg atttattcag gaagtctgta ccgtttcctg aaagtgtttg
     3301 tgggtcaact caaccctgta gtaaaccaat cccgtcaatc agtgcaccgc gccaccatgc
     3361 gtaatcgtga ccaccggtgt agattgcgca atggctttcg acgccagcgg taatcagccg
     3421 ctggtgaagc tgctgaacgt gaggcactgt cgaaccttcc agcgatccca cgcacaggct
     3481 gatacgtacg ccctgcggtg gggcagaaag cagatgctca ctcacccatg aggtatcggt
     3541 ttcgctgaac aagcctggtc gactggttct ttctggcgtc caccacattg aaggagagtg
     3601 gctgagcacc agaccgaacg tttccggtgc gtaacgagcc cccattagcg cactgatccc
     3661 gccgaggctc tgcccggcca gcactgtgcg cgaacggtct gcccactgac gctgcggttg
     3721 ttcagcgcga atcatcggca gcagatgtcc ggcgatatcc tttatcagtt tgctgcgccc
     3781 gccgagtatc tcagtgcgtt catgttcatt aatgttgtca atgcccagta cagccacggg
     3841 cacgatgcgc ccgttattta tggcggcgtc aattgccgcg catacgccaa ggtgatcaaa
     3901 ccaggtttca ccatcgggca gcacgaccag cccgaggggc tgagaaatat caacatccgg
     3961 aatatagagc cgaacctggc gagatttccc ggcgataatg cgcatggagg taagcagttg
     4021 tccggtgtgg aagccgccgt tccattccga cagggcggga gctttatcaa gtgtcagcac
     4081 tgattccttt gcgttccccc gaacgttgat ctccggcatt ttgtttagcg gatcggcttt
     4141 tccggcgagg gtggcaaaac ggcctccgga cagtgcaatc gtctcagccg tagtgccggg
     4201 ggggatttcc agcagcgaat aggagccgca gtaacttgcg ggtaaacgca gtgtcagtgt
     4261 ccagatatcc gtttcgggaa gggcgctcat cattcctttt tctacgtgct ctttatccgt
     4321 cacccggttc agacgcagat aaacgccctg cagaggatgt cgggcgcgcc acagaaaggt
     4381 cacttctcgc tcatcttccg ctccttcgac tggcgtcaca agaggaacat tcagcgactc
     4441 agcttcgcgc cagaagtcag taaggttagc aatgtggccc gctgccagtt ggttgcgcag
     4501 gctggcgata gcagagggat gttgttgcat gttcagcatt catactcttt aaaaattaag
     4561 gatgtcagta taaataatta aagcggctat tcacggtacc gccgcttctg actgtgtttt
     4621 taatacatta cgtgtccagt aactatcccg gggtttcctc cttcatgttg tgctggcgat
     4681 ggagcgcagt actgctgacg ctgtcatgcc ataagcgggt atagaggccg cccgcagata
     4741 acagttgttc gtgggttccg tattccgcaa gtaacccctt atcaataacg gcaatcagat
     4801 cgcagcgttg cgcggtggtc agacgatgtg caacaatcag cgccgagtgc ttcccggcat
     4861 gcacgacatc tgttaacgat gacatcagtc gttcttcgga tgtgcgatcc agacatgacg
     4921 tggcttcgtc gagcagcagg atgtgagcat tcgccagttg cgcgcgggcc agcgcaatca
     4981 actggcgctg acctgcggac agatcggctc cgccgttatt cactggtgtc cggaatccct
     5041 gcggcagatt gcacaccatc tcatacagcc ctgcccgctg tgaggcaatt tcaacgtctt
     5101 cattggtggc ggatggccgt gaataacgaa tgttttctgc aatatcacta ctaaacagtg
     5161 ctacatcctg agcgacaagc ccaatctggc gacgatactc agtgagcgag gcatcatcga
     5221 gcatttgcac accgattctg atgttgccgt gcgtggggaa atacaacccg gcaatcagct
     5281 taatcagcgt cgacttaccc gcaccgctgc gaccgacgac cgcgacaacc attccctctg
     5341 ggatcgtcag ggtaagtttg ttcagagctg gctcgtgact gtcgggataa ctgaacgtga
     5401 cctcatcaag atgcagtgca ccggtgacag ggaggaccga agaggagccg aggttctcag
     5461 tgccttctgt cgccagtagt tcatcaatat gtttgccgct ggctgtcgcc tgctgccagg
     5521 cgtcgacaat ccctgataac tgctgcactg gcccatagaa ttgccccagc aacaggaaga
     5581 aagccgccag taccccggca gtcatttctc cagcggcgac ctgcgaagct cccactaaca
     5641 gaacagcggc ataggaggcc tcggtgcaga atgtcaggaa cggaaaatag actgcaaggt
     5701 atttttgtgc tcgcacacgg gttgcgcgga aacgctccga taacgcgcgc agccgggcgc
     5761 cctccagttc ctgctgacca tgcgattgca cgacacgcag gccggagact ttttcctgca
     5821 gggtgctgtt gactttgcct atttccagcc gtgcctgtgc ataagcaggg gtactgaggc
     5881 ggcgataaat catggttgcc agtgcggcca ctggcactgc gcttaatgcc gtcagcgcca
     5941 ggaacgggtc gagccagaac atggtggcgg cgattgcgaa catcgtcacc agactggtgg
     6001 ccgcaccggc aaggccgttt tgcagaaagc gggcgagaga gtccacatca acggtcatgc
     6061 gggtaagacg cgaatcggca tgcttttcat gccaggggag tccaagacgc agcatatggc
     6121 cgaagctgcg caagcgcacc gaatgctgaa ctgattccgc cgctctggcg gcgaagaccg
     6181 tctgcagaga ataactgcac cagcccacaa tgaccaggca cagggcgagc agggcacaga
     6241 ggccgatggt cgacatatcg cctgcggcga caccggagtc aatgccgtgc tgcaacagta
     6301 tcagcacccc tacgcctgcc gcagaatcga gtgcgatcag cagagcggcg atgacgaaca
     6361 tccacgccac aggcttcagt agtgacgtga ctttgcgacc tttaccggcc agcgcctgct
     6421 gtactgcatt ttccggtacg cgggtcatac gggcgacaaa acctttatcc cccgtatccg
     6481 ttacatcatc ttgtgtcgct ggttcagccg gccacaattc gttgtggcta ttgtgggacg
     6541 tggccagaaa atcgctgtcg ccggtcatca gtgcgcggaa cgccggacag cgcgcttcaa
     6601 gttctgccgg ggttccggta tccaccatac ggcccttatc cagcaccaca acccggctgg
     6661 ctagctgaag tgttgaacgc cgtcgggcaa tcaccagcag catatgccct tcgtcagcat
     6721 agcgacccag cgcggtatta atctccgctt ccgtaacagc atcaaccgct gaggtggtat
     6781 catccaggat taacacgtcc ggtgcagtaa tgagcgcccg cgccagcgct attcgctgcc
     6841 tctgcccgcc ggaaagattc gtaccccgtt cggttaacag gctatcgaag cctttcggca
     6901 gggcgttgat aaaatcgctg gctcctgcag caactgccgc acggcgaatg tcgtccgccg
     6961 ttgcctgagg gtggccatag gcgatatttt ccgccaccgt accggcaaac agaaaagcat
     7021 cttcaaatac gatgcctacc cggcgacgaa gcgcctccag tctgatatcg cgaagatttt
     7081 gtcggccttc gctggtgttg agccatatct ttccggagca gggatcataa aaacgcgcca
     7141 gcagcatcaa cagggtcgat tttcctgaac ccgatgcgcc caccaccgcc acggtttccc
     7201 cagcgcgcag ggaaaaggag atatcgctga ggatatgtct gtcgccatgg tggtagtcaa
     7261 agctcatatt ctccagttcc agtccgacaa cctgactgtt aatcgactcc gtcccgtcct
     7321 ccatctgtga acgggtgtcg atcagtgcaa acacccgctc cacggacgcc tgagtgcgct
     7381 gtgcgataac cagaaacgat gccagtacgc gggttggccc ggtcagcatc gcgaggaagc
     7441 tggcgaatgc aacgaaggta ccgagatcga tccgcccgtg catgaccgac cacccgccgt
     7501 agcacagcag gacgatttgc ccgagcaccg gtagcgccag catagtggcc ccaggcatcg
     7561 cctgcgagat aaccgcaccg atgcgtacac gcacaatctg ccgcgactgc ctatcgagcc
     7621 agtgcgtttc acgcatctct gccacacagg attttaccac tgagatctgt gccagcacct
     7681 cgcgtatatg ttcggtgaga ttcgccagtt ggtccgaggc cattccggtt tgcgcgaaga
     7741 ccctacgacg cgcacgaagc gcggtgatcg ccaggcatac cagtacgcac acgacgataa
     7801 gcgtcatggc aggggacatc cacagcatca cggcaatgcc ggcaatgtaa taagtgaaca
     7861 ctgccagcgg caccgggcac atctgcagca gggtatgcac ctgctgcaga tcgctgttgg
     7921 tccgtgaaat cacctgcccg gtgcgcagcg catcctggcc tgcgccgtca agtttctgaa
     7981 tactgtcgaa tacccgtcct ctgagggtat gctgcaccca gagtgagagc atcccggcat
     8041 aaccacggcg cacatagtta ccgatgaaat caaaaaaagc gatcaacagt aacccgcagg
     8101 ccagccacgg caggcgggcc ggattgcccg ccagtgcgtc attcacagcc tgtcttgtca
     8161 gcagtggcgt cagcgcagcc agcaaaatag tcgatactga cgctaccacc acgatgacac
     8221 taagtttctt acgttcccag cacacgcgcg ccaggcgaac tatccatgac tgagccggtg
     8281 tgggagtgtg attcgcaggc attaagcctc ctccgtctgt aaaatacgat ccactggccg
     8341 gatcgttccg caaaaaagcc agcacactcc ggtcccgcat tgttatggat gcatgactcc
     8401 tggatggggg ctggcaaatg attatcatga aggtgtagtg gctacccttt ctgtaccatt
     8461 gtgatcaggc ttttcgccac ttcaccaggg cagggttgcg ccgccatttc cgctgctacc
     8521 tcttcagagg ctttgcgaag tgagcgattg ttgaggaaag cattgatcat gttactcgac
     8581 aggccgacgt cgccggggat aatcccgcag ccgcgttcgg caacaacgcg ggcatttacc
     8641 gggcgatcgg ctccctggcc gaagactatt tgtggaatac cagcgtgtag cgctgtcagg
     8701 gtattgcctg ctccgccatg atgaatgaaa ccatctgcgc cgttgaggaa gacacccatg
     8761 ggtatccagt caaccaggcg aacatttgat ggcagcgagc gcaaatcgga ccgcgcattg
     8821 gctgaaatat gcaggatgat ctcggcatcg acctcactgg cagaatccat aacccaggcg
     8881 attagatcaa ggccgtcaac catcggtttg acagtgccga gactcaccag aagacgttta
     8941 cgatcgggcc ttctctccca ccatggttcc cacaccgcac caccgttata tgggacgtac
     9001 tgcatcggaa taatgggttc accgtcgttt tccagaatgc tcatactcgg cggtgttacg
     9061 tcgatccagg ccatatcgcg tggtgtggcc ccgacattgt ggcgacgata ggcgtccgtg
     9121 agcgaacgcg ttacacccct gatatgccag ggggtatggc cgaagcctac ggtttgcatc
     9181 acgaccggta tatcatattt ggcggcaatc agtggcccga tgactccgag tggagggtag
     9241 atgatgaggt caggccgcca gtgtccggca aactccacca gatggtctgc catttcttcg
     9301 ctgaagaatg agaagttgcc catttgggtt ccgatgttac tttttttccg ctgtgcctca
     9361 tggtggcggt aaccagcttc ggaatccaga ccgggggcag cgtcaaacac caccaacccg
     9421 gcctctgccg ctttttgtgc gaattgtcct ccgctggcga tcagtacttc atgaccgtta
     9481 acgcgaaatg cctgcgccag ggacagcaca ggatacaaca gcccatagag aggcgggcct
     9541 acaaacaaaa tacgcatgag aaaatccctc tccgcttgac gatctaaatc aggcagtcga
     9601 cgcactcata tgccagcagc aggacacgag ttttcgttaa acctgcttta ccagatgctg
     9661 cgccatttgt gcttcaccag cgttatggac agatcagtca gagacttttc cgtgacatac
     9721 acaggtgaac agggaactcc gtatccccac ttccattaac gcttacgcgc cattgtcctg
     9781 ttgaacatcc cggcgtcgga cagctacgcc gggatgttct gacgcatttg tgcagtgtta
     9841 gaccgacaat gaatctggaa agatccgtga ctttttagac acacggcttg catcatcctg
     9901 atagtctcgc cttcgctggc ctgtaagtaa aatgaccttg tactggcgat gcgtgttccc
     9961 tttcagcctt accagagaca acgttagcgc aaatgctaat gataataatt accaatatca
    10021 ttaaggtatt gcaacgataa ttaaaaactt aatgaaaagg gaagcactca gaggactatc
    10081 cgggggtatt taaattgaaa cctacgctgc tccagaccgt ggagaatatt tgcctgcaaa
    10141 ttctgttcgt gctgttcctg ttcaccggaa cgctgataga cgttgaggca ttggtgagct
    10201 gttcttccgt tgccaaaaat gtacagagaa ctccggccag cagcgtttca tcctctgtct
    10261 atagcgcaag cgtgtcgaga actgctgctg atattatgct gttgtcgaat aatccgcttt
    10321 ttgctccggg tcaggtgagt tttttcggct gttcgttggc agcgtcaggt attcacgtca
    10381 ccattctgga gtaatgccag tcaattaagc aactgactgg ctctttttgt gcacagaaaa
    10441 cccccagcta ggctgggggt tccggaaagc tttcagcttt gagccagtta ttaaaacccc
    10501 ttttgatttg ttaaaacacc ttgcggtctg gcaactgcaa gtgtcaaaca agaaatcaaa
    10561 agggggtccc aatggggaac gaaaagagct tagcgtacac ccgatggaac tgtaaatatc
    10621 acatagtatt tgcgccaaaa taccgaagac aggtgttcta cagagagaag cgtagagcaa
    10681 taggctgtat tttgagaaag ctgtgtgagt ggaaaagtgt acggattctg gaagctgaat
    10741 gctgtgcaga tcatatccat atgcttgtgg agatcccgcc caaaatgagc gtatcaggct
    10801 ttatgggata tctgaaaggg aaaagcagtc tgatgcctta cgagcagttt ggtgatttga
    10861 aattcaaata caggaacagg gagttctggt gcagagggta ttacgtcgat acggtgggta
    10921 agaacacggc gaagatacag gattacataa agcaccagct tgaagaggat aaaatgggag
    10981 agcagttatc gattccctat ccgggcagcc cgtttacggg ccgtaagtaa cgaagttgga
    11041 tgcaaatgtc agatcgtgtg tgtcacgaac ggtgcaatag tgatccacac ccaacgcctg
    11101 aaatcagatc cagggggtaa tctgctctcc tgattcagga gagcttatgg tcacttttga
    11161 gacagttatg gaaattaaaa tcctgcacaa gcagggaatg agtagccggg cgattgccag
    11221 agaactgggg atctcccgca atacggttaa acgttatttg caggcaaaat ctgagccgcc
    11281 aaaatatacg ccgcgacctg ctgttgcttc actcctggat gaataccggg attatattcg
    11341 tcaacgcatc gccgatgctc atccttacaa aatcccggca acggtaatcg ctcgcgagat
    11401 cagagaccag ggatatcgtg gcggaatgac cattctcagg ggattcattc gttctctctc
    11461 ggttcctcag gagcaggagc ctgccgttcg gttcgaaact gaacccggac gacagatgca
    11521 ggttgactgg ggcactatgc gtaatggccg ctcaccgctt cacgtgttcg ttgctgttct
    11581 cggatacagc cgaatgttgt acatcgaatt cactgacaat atgcgttatg acacgctgga
    11641 aacctgccat cgtaatgcgt tccgcttctt tggtggtgtg ccgcgcgaag tgttgtatga
    11701 caatatgaaa actgtggttc tgcaacgtga cgcatatcag accggtcagc accggttcca
    11761 tccttcgctg tggcagttcg gcaaggagat gggcttctct ccccgactgt gtcgcccctt
    11821 cagggcacag actaaaggta aggtggaacg gatggtgcag tacacccgta acagttttta
    11881 catcccacta atgactcgcc tgcgcccgat ggggatcact gtcgatgttg aaacagccaa
    11941 ccgccacggt ctgcgctggc tgcacgatgt cgctaaccaa cgaaagcatg aaataatcca
    12001 ggcccgtccc tgcgatcgct ggctcgaaga gcagcagtcc atgctggcac tgcctccgga
    12061 gaaaaaagag tatgacgtgc atcctg
//
LOCUS       DAZKHK010000139        11994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKHK010000139 DAZKHK010000000
VERSION     DAZKHK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.140"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..400
                     /locus_tag="ACHOS5_004170"
     CDS             <1..400
                     /locus_tag="ACHOS5_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778898.1"
                     /translation="LSFLVLFFIFFYAYISLMSKEHHYTQHELSPFFLYTPESLRNLP
                     NISNVAEYSYYYNVDDMQTRVIVTWRNIDNIFLQKAKLIDFLKRMGPSLQNDCIWFFH
                     DKSDYANNFQRYCIIEHRDSLQVEYFETIE"
     gene            555..1319
                     /gene="yabI"
                     /locus_tag="ACHOS5_004171"
     CDS             555..1319
                     /gene="yabI"
                     /locus_tag="ACHOS5_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9778899.1"
                     /translation="MQALLEHFITQSTVYSLMAVLLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWYAWLAGIIGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(1433..2131)
                     /gene="thiQ"
                     /locus_tag="ACHOS5_004172"
     CDS             complement(1433..2131)
                     /gene="thiQ"
                     /locus_tag="ACHOS5_004172"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP9778900.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGLLTIDDVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSMSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(2115..3725)
                     /gene="thiP"
                     /locus_tag="ACHOS5_004173"
     CDS             complement(2115..3725)
                     /gene="thiP"
                     /locus_tag="ACHOS5_004173"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP9778901.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     SVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGCHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(3701..4684)
                     /gene="thiB"
                     /locus_tag="ACHOS5_004174"
     CDS             complement(3701..4684)
                     /gene="thiB"
                     /locus_tag="ACHOS5_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP9778902.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPYDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAATNFIEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(4715..4814)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4848..6503)
                     /gene="sgrR"
                     /locus_tag="ACHOS5_004175"
     CDS             complement(4848..6503)
                     /gene="sgrR"
                     /locus_tag="ACHOS5_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP9778903.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVISSLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHVRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            6592..6723
                     /gene="sgrT"
                     /locus_tag="ACHOS5_004176"
     CDS             6592..6723
                     /gene="sgrT"
                     /locus_tag="ACHOS5_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP9778904.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(6831..7436)
                     /gene="leuD"
                     /locus_tag="ACHOS5_004177"
     CDS             complement(6831..7436)
                     /gene="leuD"
                     /locus_tag="ACHOS5_004177"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP9778905.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(7447..8847)
                     /gene="leuC"
                     /locus_tag="ACHOS5_004178"
     CDS             complement(7447..8847)
                     /gene="leuC"
                     /locus_tag="ACHOS5_004178"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP9778906.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(8850..9941)
                     /gene="leuB"
                     /locus_tag="ACHOS5_004179"
     CDS             complement(8850..9941)
                     /gene="leuB"
                     /locus_tag="ACHOS5_004179"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP9778907.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYLD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVAEGV"
     gene            complement(9941..11512)
                     /gene="leuA"
                     /locus_tag="ACHOS5_004180"
     CDS             complement(9941..11512)
                     /gene="leuA"
                     /locus_tag="ACHOS5_004180"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP9778908.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(11604..11690)
                     /gene="leuL"
                     /locus_tag="ACHOS5_004181"
     CDS             complement(11604..11690)
                     /gene="leuL"
                     /locus_tag="ACHOS5_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP9778909.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         2698 a         3174 c         3199 g         2923 t
ORIGIN      
        1 attatcattt ttagttttat tctttatatt tttctatgct tatatctcat tgatgtcaaa
       61 ggaacatcac tatacacagc atgaactttc cccatttttc ctttatacgc cagagagttt
      121 gcgaaatttg ccaaacatat caaacgtagc cgagtatagc tattactata atgttgatga
      181 tatgcaaaca agagtgattg ttacatggcg aaatatagat aacatttttc ttcagaaagc
      241 aaagctaata gattttttga aaagaatggg accgagtcta caaaacgatt gtatatggtt
      301 ttttcatgat aaaagtgatt atgcaaataa tttccagcgt tattgcatca ttgagcaccg
      361 tgattctctg caggtggaat attttgagac tatcgaatga taggcagagg gtgtcttttg
      421 tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcataat tggtaacgaa
      481 tcagataatt gacggcttga cggagtagca tagggtttgc agaatccctg cttcgtccat
      541 ttgacaggca cattatgcaa gcattgctgg aacactttat tacccaatct accgtgtatt
      601 cattgatggc ggtgttattg gtggcctttc tggagtcgct ggcgctggtc ggtttgattc
      661 tacccggtac ggtgctgatg gcggggctgg gagcactgat tggcagcggt gagttaagtt
      721 tctggtacgc ctggctggcg gggattattg gctgcctgct gggcgactgg atctccttct
      781 ggctgggctg gcgttttaaa aagccgttgc atcgctggtc atttctgaag aagaacaaag
      841 cattactcga taaaaccgaa catgcgctgc atcagcacag catgttcacc attctggtcg
      901 gtcgttttgt cggcccgacg cgtccgctgg tgccaatggt ggcgggaatg ctggatctcc
      961 cggtggcaaa atttattacg ccgaatatta tcggctgctt gctgtggcca ccgttttact
     1021 tcctgccggg gattctggcg ggtgcggcga tcgatattcc tgccgggatg cagagcggtg
     1081 agtttaaatg gttgctgctg gcaacagcgg tgtttttgtg ggttggaggc tggctgtgct
     1141 ggcggttatg gcgcagcggt aaagcgactg accgtttgag tcattatttg tcccgcggtc
     1201 gtttgttgtg gctgacgccg ttgatttctg ccatcggcgt ggtggcgctg gtggtgttaa
     1261 ttcgccaccc gttgatgccg gtgtatatcg atattttgcg taaagtggtt ggggtttaga
     1321 ggatagcctt gtgcgggttg cctgatgcga cgcttgccgc gtcttatcag gcctacaaaa
     1381 cgcactacct gtaggtcgga taaggcgttc acgccgcatc cgacagtgca tattaacctt
     1441 taattcccaa cagcgccgaa gcactcgcct taccgctcaa caactcatcg gtcttaccct
     1501 gccaggcgat acgcccgtcg gcgaccacca ccgagcgcgt ggcgatccgc gccgcatctt
     1561 ccacactgtg cgacaccatc aatagcgtca ttttttgctg ttggcagctc atgctcacta
     1621 gcgtcagcat ctcctgacgt agcgccggat cgagcgcgga aaacggttca tcgagcaata
     1681 aaatgggctg ttcgcgtacc agacaacgcg ctaacgccac gcgctgtcgt tgaccgccgg
     1741 aaagctcgcc aggtaaccgc gccattaaat tatcaatccc catctggtgg gcaatcgcgt
     1801 gcattttctt ctgctgcgcc gcatttagtt tcaaccccgg atttagcccc agcccgatgt
     1861 tctgcgcgac cgtcaggtgg ctgaacaggt tgttctcctg aaacagcatc gacaccggac
     1921 ggcgtgacgg cggcgtcgtg gtgtgatcta cgtcgtcgat agtcagcaaa ccgctggctg
     1981 gcgtcagaaa accggcgatc aaattaagca gggtactttt acctgcgccg cttggcccga
     2041 ggatcgccac ctgctcgccg cgttccaccg ttaagctaaa acgcatcggc aaatggtggt
     2101 aaagccaggt gatatcagtc agttttaaca tttcgccccg gtattttttc aatcacggta
     2161 aacagcagaa aacagagcag cagcagaatt aacgcggtga ccgcgccgtc ctggctgcga
     2221 taggagccaa tttgctggta gagataaaac ggcagggtac ggaaatcatc gttaccgaac
     2281 aatgccacca cgccaaaatc accaatcgac agcacacaag caaaggccag cgcctgcgcc
     2341 agtggacgtt tcagggcgcg cagctccacc acttttaagc gtgaccagcc ttcaatgccc
     2401 agcgactgac acaacatgct gtagcgggcg gtgatatcgc gcatcgggtt ttccagcact
     2461 ttcagcgcat aagggattgc cattaacgca ttggtgaaaa tcacaatgcc gtcagcagat
     2521 tgtggcaggc cgatagtgtt gttgagcagt aaaaagaagc ccgtagccag cacaatcccc
     2581 ggcatggcga ggatcaacat gccgctcatc tccagcgcct gacccgccag cattttctgc
     2641 cgcgcccgaa gttcgcgact gctccatagc agcatcatgg tcagcactac gcacaatacg
     2701 cctgccgcca gcgcaatacg caacgaggtc cacagcgcct gccacagcac cggttgtgcc
     2761 agcacttccg gcaactggcg atttacccca tcgacgatca ccgccagtaa cggtggcagc
     2821 aacagcagca gcgccagcac aattaacgcc gtgtcgcaaa tgcgactatg cagacgatcg
     2881 tccgggtcgc gccagccttg cagcagtgtg gtaccaggcg caatggcctt actcaatcgc
     2941 tgactcaaca gcaccagccc gaggcagcac accatctgga tcagcgccag cattgccgcg
     3001 cgggcaggat cgtagtcgta actcagcgcc tgatagattg ccagctcgat agtggtcgcc
     3061 tgcggaccgc cgcccagcga tagcacggtg gcgaagctgg cgaaacagag cataaagatt
     3121 agtgcggcaa ccggcggaat ttgtcgccgt aaccacggcc attcgacgaa gcggaagaag
     3181 tgacagccgc gcatcccaag ctgggcggca agctgacgtt gttcgccggg aatgttttcc
     3241 agtgcctgga gtaataaacg gctagccatc ggcagattaa aaaacacatg ggccagcaaa
     3301 atgccttgca ggccgtaggg cgaaaaggtc cactccagac caagtgattg ccagagcgag
     3361 gccagccagc cctggcgacc atagacgcta agaatgccga aaacggcaac cagcaccggg
     3421 aggatcaagg tcattgcgca cagacgcaac agcatctgtc gcccaggaaa gcgcctgcga
     3481 tagagggcgc gggcgaggaa tattgcgggt acgacagaga gcagcgccga gagaaacgcc
     3541 tgccagaagg agaagcgcac cacatgccac aggtagctgt cctgccagac tgacacccag
     3601 tcaccctgcg gcgcgttcca ccacagggcg agaaacgccg ccagtgcaac cgctaccacc
     3661 agcgtggcgg cgcttacgcc tggaattaac cagccgggaa ttaacggctg acggcgcgtt
     3721 gccattcgct aatccatgcc tgacgttgtg ccgccacttc ggcgggtgtg aactctagtg
     3781 tggtggctgg tttggtcaat tgttcaaatc ctgcaggcag agtgacgttt gctaccggat
     3841 acatccagtt gccggttggg atcgcattct gaaaagccgg agaaaccata aactggagga
     3901 atttttgcgc cagctctggc tgcttgctgg cagcggtgcg ggcggcaact tccacctgca
     3961 gatagtgacc ttcgataaag tttgtggcgg cgtagttatc tttcttctct tcgagaatgt
     4021 gataagccgg agaggtggtg taactcagta ccagatcgct ttcgcctttt aaaaacaggc
     4081 cgtaggcttc gctccagcct ttggtgaccg tgacagtttt cttcgccagt ttctgccagg
     4141 cttgcggggc gttatcgcca tagacttttt gcatccatag caacagaccc agccccggtg
     4201 tactggtacg tggatcttca taaatcaccc gccagttttg atcgctctca accagttctt
     4261 tcaggctttg cggcggattt ttcagtttgt tcttgtcata gacgaaggcg aaatagccgt
     4321 aatcatacgg tacgaaagtg tcattattcc agccgccagg aacgttaacg gcttccgctg
     4381 ccacaccgct tttagcaaac agccctgttt tgctggcggc gtctaacaga ttgttatcca
     4441 gccccagcac cacatcggct ttgctgtttt tgccttccat ccgtagacgg ttgagcagcg
     4501 aaacgccatc ttccagcgcc accagtttca gttcgcaatt acagtctgct tcaaaggctt
     4561 ttttgactac cggaccaggt ccccagtcag cggcaaagga atcgtaggta taaacagtta
     4621 gaacaggttt agcgaaaaca ggcgctgtgc acagcaacag caggggaaga cattttttta
     4681 acactttgca cctcacaaaa gagtggcaaa ggacttgaga aggagcctca aatcccttcg
     4741 ccggcgttat ccggatcagg ttcgacgggt attttctcag cgcacgcggc tgcgtggcac
     4801 cccgttgaga acggcgttag tgtagtgatt ttgttatcaa ccagcaatca tggatccggt
     4861 ggtgcaaacc acgctgattt aaaatcgaac cagccgaggg tgttcatgcg caggccgcgc
     4921 atactacgtt gcccctgaat gatcagccag tggtggataa gcggcaccat tgctttgctg
     4981 gcgaccagtt gttggcacca gttcgccagg ttcatttcgc cattgcgcca gcgggcggcg
     5041 tcggcttgcc agtcgatcgg aatacagtgt tggagcagcg gcacctcgca caagtgcgcg
     5101 aacagcgaaa aatccagcgg cagcgtaaag ttggcgctgt taagccagat atcgctctcg
     5161 atctctcctt catgccactg atcgtagctg atctctttga tttccagcgt gacctggtga
     5221 cttgccagaa tctgctgcat gatcccggca atcacccgat gctcgctgtg atcctgataa
     5281 aaggtcaggg tgaggctttc cagaccagcc ggtttttcgc tctttatggt gcggacatgg
     5341 tgccaacggg ggagcagtcc ataagccggg aaccacagtt gctggtactg ttcctcagcg
     5401 aaatagacca gattcgtcgg agaaagcaca tagcttaccc agtctctgac ttgctgattc
     5461 gcccctcgat gggtgcggct gtcgaacagt aaatagtagc aaccttcctc caggcggctt
     5521 tcaatctctt tttcctcgcc ctgtggacct tttagcatca gccctccggc tggctcgtcg
     5581 gcaatttccg gcagaaccca gacgttaact tcgtcgatta atgcccggta accgaagaag
     5641 tcatcgaatg cctgaatttt cagttgattg gtgctgttgc ggatcaccgc atacggaccg
     5701 gtgccgatgg gatggctggc aaagttactg agggtttccc attcgcgcgg caggatcatt
     5761 gccggaactt gccccagcag taacggcaac cagcggtccg gttgcgtgag atggatatcc
     5821 agcgtccagg gcgtcggcga cacaatgtca gcaatatgcg aatagagcgg aagcgtattg
     5881 attcgtttta aagaggagat cacatcgtcc atttccagtt cacgaccatg gtgaaaatgg
     5941 actcctggac gcaaaaagaa acgccagtga agcggtgata tttgctgcca gtggtgtgcg
     6001 atgtctgctt ccagttcccc attttcctca tttatgcgcg ttagcgaact gaagatttgc
     6061 cgggcgatat gggtttcaga acggcgcaat gcgctgccag gtagcagatt acgcaacgga
     6121 cgatagtaga gcacgcgcag gatgtgccgt ccctggcgga agctgcggcc cagatgagaa
     6181 accagcattt gccgcacagt cgctttgtcg ccaaccaact gcactagttg gtcgatacga
     6241 tcctgctcca gcaggtcttc cgcccgctgt tgctgaagcg ccagcccggt atagaggaat
     6301 gtcaggcgtg aacgtttacc gcgcccgact tccgcttccc acgtcagcca gccgcgatcc
     6361 tgcatggtgt tgagcagggt gcgcatatga cgacgcgagc aactcagtaa cgctgccagt
     6421 tcgttgagcg tggtctcctg agatttaccc tcgcagcatt gccacaggcg gatgaactgt
     6481 tgttgcagac gagcagatgg cataaaaggg gaactcctgc gcaaaagaca gcaattttat
     6541 tttccctata ttaagtcaat aattcctaac gatgaagcaa ggaggtgccc catgcgtcag
     6601 ttttatcagc actattttac cgcgacagcg aagttgtgct ggttgcgttg gttaagcgtc
     6661 ccacaacgat taaccatgct tgaagaactg atgcagtggg aggggagtca ttctgactac
     6721 tgatttgcgg acatcatgtg tgactgagta ttggtgttaa tcgccacgcc agcagtgatt
     6781 atctgctggc ttttttcttt ttggcgattg agtgccggac ggtgtacaaa ctaacgcata
     6841 aacgccggtt gcttctcttc ataagacgca attgcgtcgt cgtgctgcag agtcaggccg
     6901 atgctgtcca gaccgttcat catgcagtgg cggcggaagg catcgatggt aaagcgataa
     6961 gttttctctc ccgctttcac ctcttgcgct tccaggtcta catcaaaatg aatccccgga
     7021 ttggctttca ccagcgcaaa cagctcatcc acttctgcat cgcttaattt caccggcagc
     7081 agctggttgt taaagctatt gccgtagaag atgtcagcaa aactcggcgc aatcaccacc
     7141 ttaaaaccgt aatcggtcaa tgcccacggc gcgtgctcac gcgaggaacc gcagccgaag
     7201 ttttctcgtg ccagcaaaat ggaagcgccc tgatactgcg ggaagttcag cacgaagtcc
     7261 gggtttggct gttggccttt ttcatccaga aaacgccagt cgttaaacag atgcgcgcca
     7321 aaacccgtac gggtcacttt ctgcaaaaac tgtttcggga tgattgcatc ggtatcgaca
     7381 ttggcggcat ccaacggaac caccaggcct gtgtgtttga taaatttctc tgccatggtg
     7441 tgctccttat ttaatgttgc gaatgtcggc aaaatgaccg gtcacagccg cggcggcagc
     7501 cattgccggg ctgaccagat gcgtgcgccc gccgcgtccc tggcggcctt caaagttacg
     7561 gttgctggtg gaggcgcaac gttcgcccgg attcaggcgg tcgttgttca tcgccagaca
     7621 cattgaacaa cctggcaagc gccattcaaa accagcttcg ataaaaattt tgtccagacc
     7681 ttccgcttcc gcctgggctt taaccggacc ggagccggga accaccagcg cttgtacgcc
     7741 tggcgcgact ttccggcctt tggcgatttc cgctgctgca cgtaaatctt caatacgcga
     7801 gttagtacag gaaccaataa acactttgtc gatagccact tctgtcagcg gaatacccgg
     7861 tttcagcccc atataggcca gcgctttttc tgccgacgcg cgttcaaccg gatcggcaaa
     7921 cgaagccgga tcgggaatat tgtcgttcac agaaatcacc tggcccggat tggtgcccca
     7981 ggtgacctgc ggcgaaatct cttctgcttg cagagtgaca acggtatcga aagttgctcc
     8041 ttcgtcggtt tgcagggttt tccagtaggc aacggcgtcg tcgaaatctt tgcctttcgg
     8101 cgcatgcaga cggcctttaa catagttaaa ggtggtttcg tccggtgcaa ccagaccggc
     8161 ttttgcgccc atttcgattg ccatattgca cagggtcata cgaccttcca tgcttaaatc
     8221 acggattgct tcgccgcaaa attccaccac atgcccggta ccgcctgcgc tgccggtttt
     8281 accaataatt gccagcacga tatcttttgc ggtaatgccc ggcgcggctt tgccctggac
     8341 ttcaattttc atggtcttcg cccggccctg tttcagggtt tgcgttgcca gtacgtgttc
     8401 aacttcggaa gtgccgatac caaaggccag tgcgccaaac gcgccgtggg tggcggtatg
     8461 tgagtcgccg cagacaatgg tcatccccgg caatgtgacg ccctgttccg gccccattac
     8521 gtggacgatc ccctgatacg ggtgattcag gtcatacagc tcgacgccaa attctttgca
     8581 gtttttgatc agctcctgca tctggatgcg cgccatttca ccgcaggcat taatgtcttt
     8641 ggtctgagta gagacgttgt gatccatggt ggcgaaggtt ttccccggct gacgtaccgg
     8701 gcgaccgtgg gcgcgcagac catcgaacgc ctgcggtgag gtcacttcat gcaccaggtg
     8761 gcggtcgata tataacagcg gggtttcgtt ttcggcttcg tacactacgt gagcgtcgaa
     8821 caatttttcg tataacgtct tagccatgat tacacccctt ctgccacata gcgggcaatg
     8881 atatcgccca tttcattggt actaacggcg gcagcgccac gggctaaatc tccggtgcga
     8941 atgccttctt ctaatgcgcg gttaatggcg cgttcaatgg cggaagccgc atcatcggca
     9001 tccaggctgt aacgcagcag cagtgccagc gacaggattt gtgcaatcgg gttggcgatg
     9061 tttttgcctg cgatatctgg tgcggagccg cctgccggtt catatagacc aaaaccttgt
     9121 tcgttcaggc tggcggaagg taacatcccc attgagccag tgatcattgc gcactcgtca
     9181 gacagaatgt cgccaaacag gttggagcac agcagtacgt caaactgcga cggatcttta
     9241 atcagctgca tggtggcgtt gtcgatgtac atatgcgcca gttcgacatc caggtattcc
     9301 gtggcgatct cgttaacgat ctcacgccat aaaatagagg attgcagcac gttggctttg
     9361 tcgatagacg tcactttatg gcgacgttta cgagcagatt caaacgcgat gcgggcaata
     9421 cgctcgatct caaaacggtg atacacctcg gtatcaaacg ctttttcata ttgtccgcta
     9481 ccttcgcggc cttttggctg accgaaatag atgccgccgg tcagttcgcg cacgcacagg
     9541 atgtcgaagc cgttagcggc aatgtcagca cgcagcggac agaatgcttc cagaccctga
     9601 tacagttttg ccggacgcag gttgctgaat aatttgaagt gcttacgcaa aggcaacagt
     9661 gcgccgcgtt ctggttgctg gtctggtggt aaatgttccc acttcggacc gcctaccgag
     9721 ccaaacagca cggcatcggc ttgctcacaa ccttcaaccg tcgcaggcgg cagcggctgc
     9781 ccgtggttat caatggctgc gccgcctaca tcgtaatggc tggtggtgat acgcatcgca
     9841 aagcggttgc gcacggcatc cagcactttc agcgcctggg tcatcacttc cggcccgatc
     9901 ccatctccgg gcaaaacggc aatatggtaa ttcttcgaca tcacacggtt tccttgttgt
     9961 tttcgttgtg ttgagctttg cgttgcaact ctttttcgac ttctgcggcg cgccagatat
    10021 tgttcagtac gtgcaccatg gctttggcgg aggactcgac aatatcggtg gccaggccga
    10081 cgccgtggaa gcggcgacca ttgtagttgg cgacaatatc cacctgaccc agcgcatctt
    10141 taccgtgacc tttggcggtc aggctgtatt tcaccagttc gacgttatag tcagtgatgc
    10201 ggtttatcgc ctgatagacg gcatcgaccg gaccgttacc gttggcggct tctgctttga
    10261 cttcttcgcc gcaggccagt ttgacggcgg cagtggcaat atcgttagag ccagactgca
    10321 cgctgaagta atccagacgg aaatgctctg gctcttcttg ctgcttaccg atgaaggcca
    10381 gcgcctccag atcgtaatca aacacctggc cttttttgtc cgccagcttc aggaaagcgt
    10441 cgtacaggtt gtctaaatta tattcacttt ctttatatcc catctcatcc atgcgatgtt
    10501 tcaccgccgc acgccccgaa cgagaggtca gattcagctg gatttggttc agaccaatag
    10561 attctggtgt cataatttcg tagttttcgc ggtttttcag cacgccatcc tggtggatac
    10621 cggaggagtg tgcgaatgcg ccgctgccaa caatggcttt gtttgccggg atcggcatat
    10681 tacaaatctg gctaactaac tggctggtgc gccatatctc ctggtgattg atggcggtgt
    10741 ggacgttgag aatatcctta cgaactttga tcgccatgat gacttcttca agggaacagt
    10801 taccggcacg ctcgccgatc ccgttcattg cgccttctac ctgccgtgct ccggcatgta
    10861 ccgccgccag tgagttgccg accgccaggc ccaaatcgtc gtgggtatgt acggaaataa
    10921 tggctttgtc gatgttgggt acgcgttcat acaggccgct gatgattccg gcgaactcaa
    10981 atggcatggt gtagcccacg gtgtccggaa tgttgatggt ggtggcaccg gcattaatcg
    11041 ccgcttcgac cactcgcgcc agatcggcaa ttggggtgcg tccggcatct tcgcaagaaa
    11101 attcaacatc atcggtgtaa ttacgggcgc gtttcaccat atagatagcg cgttcgatca
    11161 cttcgtccag cgtgctgcgc agcttggtgg cgatgtgcat tggcgaagtg gcaataaagg
    11221 tatgaatacg gaaggcttcg gcgactttca gggattcggc tgccacgtca atatcttttt
    11281 ccacgcagcg agctaacgcg catacacggc tgtttttaac ctggcgagcg atggtctgca
    11341 ctgattcaaa atcacccggc gaagagacgg ggaaaccgac ttccatcacg tcaacaccca
    11401 tacgctcaag ggccagcgca atttgcagtt tttctttcac actcaagctt gcctgtaacg
    11461 cctgttcacc gtcgcgcaat gtggtatcga aaataatgac ttgctggctc atggtttggg
    11521 tccttgtctc tttttgagcg cctcgcttcg ggcataaaaa aacccgcgca atggcgcggg
    11581 tttttgtttg actgcgtgct ggcttaatgc tggatgccgc tcactcgtct accgcgcaaa
    11641 gaagatgcgt ttagtagtag tagaccgata aagcgaacga tgtgagtcat taaatcagct
    11701 ccagatgaat gcgatatgct tttagagtta ctggatacaa aaacggatgt caaccctgaa
    11761 gcaataaaaa cgtcacgcca ttgtgagttc ggcatccgta aaattagcta attgtgctgc
    11821 ggtggttaaa acaagcgata ttaatttctg cttaaccgcc gatgcttttc atcggttgac
    11881 atatttcaga ataatttttt gcatctaatc aacgagggaa agggggataa aatgcacgcg
    11941 ttacaaaacc tatcctgatg atttgtattg aattatatgt tttgcgaatt tttt
//
LOCUS       DAZKHK010000140        11811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKHK010000140 DAZKHK010000000
VERSION     DAZKHK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.141"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            148..948
                     /gene="ydcF"
                     /locus_tag="ACHOS5_004182"
     CDS             148..948
                     /gene="ydcF"
                     /locus_tag="ACHOS5_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP9778910.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1145..2584
                     /gene="aldA"
                     /locus_tag="ACHOS5_004183"
     CDS             1145..2584
                     /gene="aldA"
                     /locus_tag="ACHOS5_004183"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9778911.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2626..3627)
                     /gene="gap"
                     /locus_tag="ACHOS5_004184"
     CDS             complement(2626..3627)
                     /gene="gap"
                     /locus_tag="ACHOS5_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9778912.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3816..4346
                     /gene="cybB"
                     /locus_tag="ACHOS5_004185"
     CDS             3816..4346
                     /gene="cybB"
                     /locus_tag="ACHOS5_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP9778913.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKCPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4591..4764
                     /locus_tag="ACHOS5_004186"
     CDS             4591..4764
                     /locus_tag="ACHOS5_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778914.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4790..4858
                     /locus_tag="ACHOS5_004187"
     CDS             4790..4858
                     /locus_tag="ACHOS5_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778915.1"
                     /translation="MNPLVRCAGKIPALALTWTCRP"
     gene            complement(4836..4937)
                     /locus_tag="ACHOS5_004188"
                     /pseudo
     CDS             complement(4836..4937)
                     /locus_tag="ACHOS5_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            4948..5391
                     /gene="trg"
                     /locus_tag="ACHOS5_004189"
                     /pseudo
     CDS             4948..5391
                     /gene="trg"
                     /locus_tag="ACHOS5_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(5429..6352)
                     /gene="ydcI"
                     /locus_tag="ACHOS5_004190"
     CDS             complement(5429..6352)
                     /gene="ydcI"
                     /locus_tag="ACHOS5_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9778916.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6569..7912
                     /gene="ydcJ"
                     /locus_tag="ACHOS5_004191"
     CDS             6569..7912
                     /gene="ydcJ"
                     /locus_tag="ACHOS5_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9778917.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHLSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETRACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8137..9792
                     /gene="opgD"
                     /locus_tag="ACHOS5_004192"
                     /gene_synonym="mdoD"
     CDS             8137..9792
                     /gene="opgD"
                     /locus_tag="ACHOS5_004192"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP9778918.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            9932..10156
                     /gene="ydcH"
                     /locus_tag="ACHOS5_004193"
     CDS             9932..10156
                     /gene="ydcH"
                     /locus_tag="ACHOS5_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP9778919.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEITRKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            10219..10758
                     /gene="rimL"
                     /locus_tag="ACHOS5_004194"
     CDS             10219..10758
                     /gene="rimL"
                     /locus_tag="ACHOS5_004194"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP9778920.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(10750..11730)
                     /gene="ydcK"
                     /locus_tag="ACHOS5_004195"
     CDS             complement(10750..11730)
                     /gene="ydcK"
                     /locus_tag="ACHOS5_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP9778921.1"
                     /translation="MRKYRLSEEQRAFSYQDDGTKKSVLLRQIIAMSDFNDVIAGTAG
                     GWIDRETVLSQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTLLAGLL"
BASE COUNT         3029 a         2799 c         2969 g         3014 t
ORIGIN      
        1 gaataatgtt tatctacagc atttccttaa aaaatatgtc aggcttgcgg agtggcagtt
       61 aaggaaatac gacttcctcc tttcagagtg ctccgcttct cactattatc tcacgcagta
      121 ttcttaaggg aacgataagg aggaaccatg aatattaccc cgtttccgac gctttcgccg
      181 gcaaccatag atgccataaa tgttatcgga cagtggctgg cgcaggatga tttctccggt
      241 gaggtgccgt atcaggccga ttgcgtgatc cttgcaggca atgcggttat gccgactatc
      301 gatgcggcat gtaagattgc ccgcgatcag caaattcctt tactgattag tggtggtatc
      361 ggtcactcga caactttttt gtatagcgcc atcgcacagc atccacacta caacactatc
      421 cgcaccactg gcagagctga agctaccatc ctggcggata tcgctcatca gttctggcat
      481 attccgcatg aaaaaatctg gattgaagac cagtcaacaa actgcggtga aaacgcacgc
      541 tttagcatcg cgctattgaa tcaggccgta gaacgagttc atacggctat cgttgttcag
      601 gaccccacca tgcagcggcg cacgatggcg acgttccgcc gtataaccgg ggataatccc
      661 gatgcaccac gctggttaag ttatcccgga ttcgttcctc agttaggaaa taacgcagac
      721 agtgtaatct ttgttaatcc gttgcaagga ttatggccgg ttgagcgtta tctatcgcta
      781 ctcactggcg agctgccgcg tttacgcgat gatagcgatg gctacggccc ccgcgggcga
      841 gattttatcg ttcacgttga ttttccggca gaagtcatcc aggcatggca aacgctgaaa
      901 cacgatgcgg tgctcatcga ggcgatggaa agtcgctcgt tacgttaaaa attgcccgtt
      961 cgtgaaccaa ttgtttgcaa acgggcatga cttctgactt ttatttctgc ctttaattcc
     1021 ttttacactt gtttttatga agcccttcac agaattgtcc tttcacgatg ccgtctctct
     1081 gataattgat gttaattaac aataaattta ccaaaaacaa acatgtaaat cacaggagtc
     1141 gcccatgtca gtacccgttc aacatcctat gtatatcgat ggacagtttg ttacctggcg
     1201 tggagacgca tggattgatg tggtaaaccc tgctacagag gctgtcattt cccgcattcc
     1261 cgatggtcag gccgaggatg cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga
     1321 atgggaagcg ttgcctgcta ttgaacgcgc cagttggttg cgcaaaatct ccgccgggat
     1381 ccgggaacgt gccagtgaaa tcagtgcgct gattgtcgaa gaagggggca agatccagca
     1441 gttggctgaa gtcgaagtgg cttttactgc ggactatatc gattatatgg cggagtgggc
     1501 acggcgttac gagggcgaga ttattcaaag cgatcgtcca ggagaaaata ttcttctgtt
     1561 taaacgtgcg cttggcgtga ctaccggcat tctgccgtgg aactttccgt tcttcctcat
     1621 tgcccgcaaa atggctcccg ctcttttgac cggtaatacc atcgtcatta aacccagtga
     1681 atttacgcca aacaatgcga ttgcattcgc caaaatcgtt gatgaaatag gccttccgcg
     1741 cggcgtgttt aaccttgtac tcgggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa
     1801 cccaaaggtc gcaatggtca gtatgacagg cagcgtctct gcaggtgaaa agattatggc
     1861 gactgcggcg aaaaacatca ccaaagtgtg cctggaattg gggggtaaag caccggctat
     1921 cgtaatggac gatgccgatc ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat
     1981 taatagtggg caagtgtgta actgtgcaga acgtgtttat gtacagaaaa gcatttatga
     2041 tcagttcgtc aatcggctgg gtgaagcgat gcaggcggtt caatttggta accctgctga
     2101 acgcaacgac attgcaatgg gaccgttgat taacgccgcg gcgctggaaa gggttgagca
     2161 aaaagtggcg cgcgcagtag aagaaggggc gagagtggcg ttgggtggca aagcggtaga
     2221 ggggaaagga tattattatc cgccgacatt gctgctggat gttcgccagg aaatgtcgat
     2281 tatgcatgag gaaacctttg gcccggttct gccggtggtc gcatttgaca cgctggaaga
     2341 ggctatctca atggctaatg acagtgatta cggcctgacc tcatcaatct atacccaaaa
     2401 tctgaacgtc gcgatgaaag ccattaaagg gctgaagttt ggtgaaactt acatcaaccg
     2461 tgaaaacttc gaagctatgc aaggcttcca cgctggatgg cgtaaatccg gtattggcgg
     2521 cgcagatggt aaacatggct tgcatgaata tctgcagacc caggtggttt atttacagtc
     2581 gtaatgagtg aaagaggcgg aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa
     2641 tttttcgagg gtgcgaataa gctgcgtgac gaagccatat tcgttatcgt accaggcgac
     2701 cgttttcacc agttgtaaat cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac
     2761 cgaaccgaaa tggctgccaa tgatatcgga agagactatt tcttcatcag tataaccaaa
     2821 tgactcgtta ttgtttgtcg cctttttaag cgcgttgttc acctcttcgg cagtcacttt
     2881 ctttccgaga atcgacacca gttcagtgac cgaacctgtt ttcaccggca cgcgttgcgc
     2941 atgacctttc agtttgccgc tcagttccgg gatcaccagg ccaatggctt ttgccgcccc
     3001 cgtagtgtgg ggaatgatat tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg
     3061 cgggccatcc accagtgact gggtgccggt ataggcatga atggtcgtca tcgtgccgac
     3121 ttctatcccg aaactgtcat gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca
     3181 tgacgccacg gaaacaatgg tgtcgttgcc atccagagtg tcgtcattga cgttataaac
     3241 gatggttttc atttcaccgg caggggcgga aatcaacacc ttcttcgcgc cagcatcaag
     3301 atgcgcctgc gatttctcgg cggaggtata aaagcctgta cattcgacaa tgatttctgc
     3361 gcctttcgct ttccacggaa tatttttagc ctctttttcg gcgtaaaccg cgatactttt
     3421 cccatcaacg ataagtgaat cttccgtata atcaacgctc caggggaacg gtccgtagtt
     3481 tgaatcatgt ttcagcaggt aggcgagaat ttttggggaa gtgagatcat taatagcgac
     3541 aacgtctatg ttgcttttga cttcaagtaa tcgacgcaac accagtcgac cgatacgacc
     3601 aaaaccatta ataccaactt tactcatggt cttctcctgt caggaacgtt cggatgaaaa
     3661 ttgatccttt ccaggcttag accaggatgg cgggatgggc aatctacatt cttgcagcga
     3721 aacgtaacgt actgaaaacg ggtgaacaat atttaatgaa attttaagaa aagcccgtta
     3781 tgttaacggc aaattatgtt aaagcaggaa atgttatggg aaataaatac tcaaggttac
     3841 aaatcagcat tcactggctg gtctttttgc tggttatcgt agcgtattgc gcaatggagt
     3901 ttcgtggttt cttcccacgt agcgatcggc ccctcatcaa catgattcat gtttcctgtg
     3961 gcatctcaat tctcgtactg atggtggttc gtcttttgtt aaggctgaaa tgcccaaccc
     4021 cgccgattat acctaagcca aaaccgatga tgacgggact ggcgcatttg ggccatttgg
     4081 tgatttatct cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccggg
     4141 gcaacccgtg gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac
     4201 gggttgatag cttaaagtcg tggcatgaaa cgctggcgaa tctgggatat tttgtcatcg
     4261 ggttgcacgc tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac
     4321 gcatgatgcc gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg
     4381 acccgtggta caggcgaaga atacgggcct acatcggaag cgcctattat atttatttgt
     4441 atgacaaata aaaccataat ccttgtctat acgtccatct ggcttatctt taatcaattc
     4501 acccgatctt tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcga
     4561 acataagatt cgataaatca tggaggttat atgaaaaaac tggcacttat cctggttatg
     4621 ggaacgcttg tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg
     4681 gggatctcac actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgca
     4741 tcgaaaaaaa catgcactaa ctgaagtgta aaaggggtgc catgagaaaa tgaatccgct
     4801 agtcagatgc gcggggaaaa tccccgcgct tgcccttacc tggacgtgca ggccatgagc
     4861 gcagcaactt ccttatcacc gtcccggaac cgcagttcgt agaacgattg tcgggtcagt
     4921 gtgaatgtca gaatcgtgtt actctcgcaa ggccgtggat ttgccgttgt cgccagcgaa
     4981 gtacggacac tcgcaagccg cagcgcccag gcgacgaaag agattgaagg cttgatcagt
     5041 gaatcagtca ggttaattga cctgggatcg gatgaggtgg caacggcagg gaaaaccatg
     5101 tgcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat cgccaccgcc
     5161 tcggatgaac aaagtcgagg cataacgcag gttagccagg cgatttctga aatggataag
     5221 gtgacgcaac agaatgcttc tctggtagaa gaggcctcaa cggcggcggt gtcccttgaa
     5281 gaacaggcgg cacgactaac tgaggcggtg gacgtattcc gtctgaacaa acattctgtg
     5341 tcggcagaac cacgaggagc gggtgaacca gttagttttg ctacggtgtg aaaatgttaa
     5401 aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga atagcgttaa
     5461 tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt ccaatcggtt
     5521 cgcctgcacc atgtcccgga acaggcaggg ctaccagcgt ggcatgacgc aggtcgtctt
     5581 ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt tgacgagata
     5641 gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acatccctgg ctctgcacta
     5701 atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc ggccattcca
     5761 gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg acaaccagct
     5821 tcagcgattc aagaaacagc agttcgtaat taagcccgtt catcagttca ggatctgaca
     5881 tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc atagggttac
     5941 tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga cctataaccg
     6001 aaggtaatat ccctagtgcc gcagtaggta gcgcaccaac cctgacgaca tcattattaa
     6061 gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga actctgactg
     6121 catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga ccacgctcaa
     6181 acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc gcaggttgac
     6241 tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca gctacgaatg
     6301 tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc ataagcgatg
     6361 ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttt attatgataa cttgattgca
     6421 aaatattatt aacaattaaa gcaactatgt tacagcaaaa tggataatat tgatgttttc
     6481 gcggcgagat cacagtttgt aaattcttcc agccggagtg aatgcggtta cctacactcc
     6541 agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg atgagattcg
     6601 ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc agtacggcac
     6661 gctgctggaa ctggtagctg atgtaaatct ggcggtgctg gaaaacaatc ctcaactgca
     6721 cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc atggggcgat
     6781 tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca ttatggggat
     6841 gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt cgacagcatt
     6901 tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta cctcgttact
     6961 ccgtcttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga ttctgcgtca
     7021 gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc agcagggcgg
     7081 ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt ttcgctggca
     7141 cctgtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac atcggttgat
     7201 tgctgatgtg gtctgttttc ctggatgcca tatcaaccat ctgacgccac gcacgctgga
     7261 tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaag ttctgatcga
     7321 agggccgccg cgccgcgagg tgccgatttt actacgccag accagcttta aagcactgga
     7381 agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct ttggtgaaat
     7441 tgagcagcgt ggtgtggcat taacgccgaa agggcgacaa ctgtatgatg atcttctgcg
     7501 gaacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg aaaccttccg
     7561 cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ctgggatggt tccggtaccg
     7621 tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cccggagacg atccacagcc
     7681 cttaatagaa cgtggttggg tagtggcgca acccattacc tatgaagatt tcttgcccgt
     7741 tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcgggcat gcaatcacgg
     7801 tgatgccagt cgcgaagcat ttgagcaggc gttggggtgt ccggttttgg atgagttcca
     7861 gctttatcag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt aaattctgac
     7921 catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat cccatcgaca
     7981 taaaaaaaat gccaatttat gcatattctc tgatttcaac aattggatta ttaataaata
     8041 ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaatg accagcgcgg
     8101 gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg atttattaaa
     8161 ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct tttttctcag
     8221 gcggcattcg cggccgattc tgatattgcc gacgggcaaa cccagcgttt tgacttctcc
     8281 attctacagt caatggcgca cgacttagcg caaacagcat ggcgtggtgc accacgtccg
     8341 ttacctgata ctctggcgac aatgacgccg caggcttata acagtattca atacgacgcc
     8401 gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt tttccatatg
     8461 ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac acatctggcg
     8521 cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcgggtgt ggataccaaa
     8581 caattagaag ggcaaagcga tctcggtttt gccggttttc gcgtgtttaa agcccccgaa
     8641 ctggcgcgcc gtgatgtagt atcattcctc ggcgcgagtt atttccgcgc cgttgatgac
     8701 acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac cgacagcaaa
     8761 gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccagg ggcaaccacc
     8821 tttactgttt atgcgttgct cgatagcgcc agcattactg gtgcctataa gttcactatc
     8881 cattgtgaga aaaatcaggt gattatggat gtggaaaatc acctgtatgc gcgcaaagac
     8941 attaaacagc tgggcattgc accgatgacc agtatgttca gctgcggtac taatgaacgt
     9001 cggatgtgcg acaccattca tccgcaaatc catgactctg atcgtctgtc catgtggcgg
     9061 ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca gttcaatgct
     9121 tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga tttctcccat
     9181 tatcaggaca ttatgggctg gtataacaaa cgcccaagtc tgtgggtgga accgcgtaac
     9241 aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga aacgctggat
     9301 aacattgtct gcttctggca gccagaaaaa gctgtgaaag cgggtgatga gtttgcattc
     9361 cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc gcgcgttatg
     9421 gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga acactatccc
     9481 gaaaaatggg cgcgacgttt tgccgtcgat ttcgttggtg gtgatctgaa agctgccgcg
     9541 ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca aatcgaaatt
     9601 ctctatattg aacccattga tggttatcgt attcagtttg actggtatcc gacttcggac
     9661 tccactgatc cggtcgatat gcggatgtat ctgcgttgtc agggcgacgc tatcagtgaa
     9721 acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt tgatgaccgc
     9781 gtgatgagtt aatcgttttt tcttcggcat cttcttcggg aggtgccgtc tggttaaaca
     9841 cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg tatttcagat
     9901 acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag atttaatatc
     9961 ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca ataaacttga
    10021 tcatgaaatt accagaaagg aaggttccga cggtcgaggg tacaatgcgg aagtggtccg
    10081 catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc agcaggtgag
    10141 cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta acagctgcat
    10201 ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact tgaattacat
    10261 gctgttgcgg aaagtcatgt cacacctctt tatcagttaa tctgtaaaaa tagaacctgg
    10321 ttacagcagt cgctaaactg gccgcagttt gttcaaagtg aagaggacac gcgaaaaacg
    10381 gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat gattttcaaa
    10441 gaagatgaac ttatcggcgt tatctctttt aatcgtattg aaccactgaa taaaaccgct
    10501 gaaatcggct actggctcga cgaatctcat caggggcagg ggatcatttc tcaggcgctg
    10561 caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt gatcaaatgt
    10621 cgggtggaca atccgcaaag caaccaggta gcgttgcgca atggttttat ccttgaaggt
    10681 tgcctgaaac aggctgagtt tctgaatgat gcctatgatg atgtgaactt atacgcgcgt
    10741 attatcgatt cacaataacc ctgccagcag cgttcgcgta atgcgctcct cgccgttaat
    10801 aacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga cggcgtggtc
    10861 ggttaactcc acatggtttt caattatcac cgcaccagta atacggctct caccttgtat
    10921 gacaacgtgc tcgtcgagca gaatcggtcc gccacgtacc acggcatttc cgccaatcaa
    10981 gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca cctgcgaact
    11041 gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg cgtgaccgta
    11101 gactttagca caatcgcata gccagacgtt gttctcttca ttaccttcaa cgctggcaaa
    11161 atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat cgccataaat
    11221 ttgtgcctgg tggacaatac gcgaggcgct aacctttgcg cgatcataaa tttgtagcag
    11281 gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt gatcaattaa
    11341 ggcgtgacca aatattctac attgcccgca aactaatgag tcacgaatgg tgacactgtc
    11401 acttatatat gcaccctgac tgatttcact attatcgatc cagacgttat ccgttgcgta
    11461 aacctctccc caaaggacgc tggtgccggt gatacgcgta ttaccggaaa tcaccgttcc
    11521 gccgaacgca atagcattct gatcgtaaat ccagcaattt ccttcctgcg ataacaccgt
    11581 ttcgcgatcg atccagccgc ctgcggttcc tgctatcaca tcgttaaaat cgctcatggc
    11641 gataatttgc cgtaataaca cacttttttt agtgccatca tcctgataac taaaggctcg
    11701 ctgttcttca cttagacgat atttacgcat agcgttttcc cacaggtgac ttactataac
    11761 cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg c
//
LOCUS       DAZKHK010000141        11753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKHK010000141 DAZKHK010000000
VERSION     DAZKHK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.142"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(112..1599)
                     /gene="yeeO"
                     /locus_tag="ACHOS5_004196"
     CDS             complement(112..1599)
                     /gene="yeeO"
                     /locus_tag="ACHOS5_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9778922.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1760..1835
                     /locus_tag="ACHOS5_004197"
     tRNA            1760..1835
                     /locus_tag="ACHOS5_004197"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1793..1795,aa:Asn,seq:gtt)
     gene            complement(1873..2823)
                     /gene="cbl"
                     /locus_tag="ACHOS5_004198"
     CDS             complement(1873..2823)
                     /gene="cbl"
                     /locus_tag="ACHOS5_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP9778923.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2925..3842)
                     /gene="nac"
                     /locus_tag="ACHOS5_004199"
     CDS             complement(2925..3842)
                     /gene="nac"
                     /locus_tag="ACHOS5_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP9778924.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4169..4244
                     /locus_tag="ACHOS5_004200"
     tRNA            4169..4244
                     /locus_tag="ACHOS5_004200"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4202..4204,aa:Asn,seq:gtt)
     gene            complement(4303..4863)
                     /gene="hxlB"
                     /locus_tag="ACHOS5_004201"
     CDS             complement(4303..4863)
                     /gene="hxlB"
                     /locus_tag="ACHOS5_004201"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFP9778925.1"
                     /translation="MSVQENTINILHELENNALKIDDSQAAQFISQIRNTRHIFLQGA
                     GRSGIAIRAFANRLLHLGFSVSVVGEISSPHTQPGDLLIIGSGSGETTSLKSLAQKAV
                     DSGVNVALVTMKADSTIGKLAQSVLVLPGSVKDDNNRVAGTFAQPMGSAFEQLCFITY
                     DAIVLALMSELGESSATMFTRHADLE"
     gene            complement(4879..5631)
                     /locus_tag="ACHOS5_004202"
     CDS             complement(4879..5631)
                     /locus_tag="ACHOS5_004202"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9778926.1"
                     /translation="MSNFAGKVILVTGGAHGIGKGIAKQFADRGAHTVIVDYNEENGK
                     ATAAELTFGDVKSLFVKTDVSDPQALVDVREKVIATFGRIDVLILNAGVAFANKVNNI
                     SFEEWNKTIAINLSGLFNTVKAFYDDFLTNKGSIVYISSGSALSGTGGGVSYPASKAG
                     GEGLIRGLAKELGPKGVNVNAIAPRLIDTGEMMRVNYPTQADLDAVLEKIPVRRLGTI
                     DDVANLAIFLADKDNSYIQGQTILMDGGRTIA"
     gene            complement(5727..6371)
                     /gene="rpe"
                     /locus_tag="ACHOS5_004203"
     CDS             complement(5727..6371)
                     /gene="rpe"
                     /locus_tag="ACHOS5_004203"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9778927.1"
                     /translation="MAKILPSIFGADILRLKDEIHFLEEEQTEILHVDLMDGTYVSNI
                     AFGPNQIAAMKKASSMIFDVHMMLANPERHIDDVINTGAEMISVHYESTPHVHYIIQK
                     IKKAGRKAGVVLNPGTPESVIEYLLDDIDYILLMTINPGQPGQTFIHKSLEKIRNTKK
                     MISGRDIQIEVDGGVNLDNVKQVKEAGADLIVVGGALFNKQPRESYQALKIAVQ"
     gene            complement(6394..7647)
                     /locus_tag="ACHOS5_004204"
     CDS             complement(6394..7647)
                     /locus_tag="ACHOS5_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9778928.1"
                     /translation="MEMLKNLMETITGMGATAILPLVIFILGLVFRMKPGAAIKSGIT
                     VGIGFIGLGLVVGLLNSSLQPAIEYYSKVGSGFTVADIGWPAVGAAAWVAPFAALVIP
                     VGIVLNLILVRLKLTKTLNVDIWNYMHFLVPGALAYFVFDSFIIGFSVAVALSIAALF
                     IGDLIAPRWQKYYGLEGTTCTTMIHIGWTLPFAWVVNKIIDYIPGLNKLDVDLNSVQK
                     RLGVFGEPAIIGIIVGALLGVLTKQAITTIVPMAMGVAGVMVLLPKVVGVLMEGLNPI
                     GKSAKEIMQKQMGKDAELNIGMDCALALGDPATVTVTVITIPLTMLCALVLPDIKIFP
                     IGVLMSIIYMTTMTVMVSNGNVVRSIISTLLFCVVVMYLGGYVAPGATQFLAGAGVGL
                     QGQGTDFVLTGPWEILTYWLSTVLH"
     gene            complement(7668..7961)
                     /locus_tag="ACHOS5_004205"
     CDS             complement(7668..7961)
                     /locus_tag="ACHOS5_004205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9778929.1"
                     /translation="MINILVACGSGVATSTLAADEVKSVCAEYGITAFKINKCSMAEL
                     SSEMAHADIVLTTNNYKGDIGKPHMSVAGFVTGINEGALRKKLGELLTELANS"
     gene            complement(7980..8423)
                     /locus_tag="ACHOS5_004206"
     CDS             complement(7980..8423)
                     /locus_tag="ACHOS5_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9778930.1"
                     /translation="MISISVADGAGLQNYDAVLEHIYNTHRETNTVKESWLKAMRKRE
                     AEYPTGIELEGYSIAIPHCDSEHASQPAIYIIRLPEAIPVNRADEDGILWVRLVVNLI
                     VTDPADQLRLLKSLFNHLQNEEFYQNLLTLPVDDAKALFVSKIIQ"
     gene            complement(8762..9517)
                     /locus_tag="ACHOS5_004207"
     CDS             complement(8762..9517)
                     /locus_tag="ACHOS5_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9778931.1"
                     /translation="MYLVDERRKLILEDLMSAGTVYVSDLARKYEVTYETIRKDLTHL
                     EQKGLLIKSHGGATLKQRAIEHPFQVREKENNSYKKAIARKALDFIPPNSSMIIGTGS
                     TTFELANLLCMKSGFKIFTDSLPVASVLIESDNQVFLFGGELRRKSSSVFGGWAVSMI
                     NDIHVDLCFIGTDGFSNMTGPSTPSSSDAFLDRTIISHSDKKYILGDYTKFERSSLFK
                     ICDWSEITALITNDHADKEGIAEVAKYTSVITC"
     gene            9866..10402
                     /locus_tag="ACHOS5_004208"
     CDS             9866..10402
                     /locus_tag="ACHOS5_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFP9778932.1"
                     /translation="MKTKIAIASDDVGFARKEEIKKYLIEEKNMEIVYDPVTCKEQGV
                     NNFARLADEMAGIIQRDECRLGIYICGTGIGFTCQINKHWGIRAVAVTNPYSAKRARL
                     SNNAQVIGLGCRVNDLEYSKMIVDAWLDNPFDFTTARDNSKKNLLEAERNDNALLVKP
                     ADVRWNMGFRPDDEKSEG"
     gene            10406..>11753
                     /locus_tag="ACHOS5_004209"
     CDS             10406..>11753
                     /locus_tag="ACHOS5_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9778933.1"
                     /translation="MELITQFINDLGNFIFIPVIFLVLMKILGRPLSECISSAIKVGI
                     GFIALTMTIKLMLEKMQPAVTGLAEATGSSLSAIDVGGAATAVMGFGSSMGAIIIPLC
                     VAVNIAMLVARLTDCVNVDVFNLHQNASMGAIVGVYSGSFLYGALTAALFHVWALIAA
                     DLGAKNNEKFFNLPKGVAISHPVANTYLLFAYPFNWIYDRIPGFRNLNVTAETIQKRF
                     GILGDPTMVGFIIGILLGFCGYGWKSPYHTIIASLQLGMYLAAVMLLLPRMTSIMMEG
                     LVPLSNVARKKLVKRFPDRQITVGMDTALIVGHPSVIAPALLLIPVIVILAVILPGNR
                     VMPLGDLSQFVFFIACMVPVFNGNIIRTWVTSIILFGGGLYIASWMAPATNEVFQKFG
                     TNPDASVMYSSLNPSANPFTGLFAALSHVGIIGYVMAGILLLSIGYLIKQKSRRQIE"
BASE COUNT         3369 a         2853 c         2353 g         3178 t
ORIGIN      
        1 actacagagg aatcgtgaga acgaggcgaa tattagcgat gcccacccac aatgtcaaag
       61 cctgtttttt aaatttgaaa tcgtttgctg aaataatctg cattttgtcg tttattccga
      121 cacaactggc tttttttcac acttttgcgg ctcggatcga gggtatttcc atagccaacg
      181 tccagtaacc attcgccagt aaaacagcac ggcccgcaca gcccagtcgg caaacattcc
      241 catccagaca ccaaccacac cccagccaag catgattccc agcacataac cgactacaac
      301 ccgacaaccc cacatgctca acatcgaaac ccacatggcg taacgggcat cacgagcacc
      361 tttaaatcca gcgggtagca cccatgaggc ggaccaaata ggcataaata aagcatttag
      421 ccaaatcaga atcacaacga catgtttaac ctgtggatcc tgggtgtaaa acgatgccat
      481 aaccccggca aagggagccg ttagccaggc gatggccgtt aatccaagag tggacagcca
      541 gaacacatgc cgcaactgaa tctctgcttg cgctatctgc cctaccccca accttcggcc
      601 tgtaatgatc gtagaagcag agccgagcgc acttccgggt aagttgataa gagccgcaat
      661 tgaaaacgcg ataaaatttc cggcaataac gctggtcccc atcccggcaa cgaacatttg
      721 ggttaataac cgaccactgg taaatagcac tgattcgaca ctcgctggaa taccaatccc
      781 catgacttcc cagataatgc taaaattcag cggtttaaaa tagctcttta atgaaatcct
      841 tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt gcaactgcgc caatataacg
      901 agaaatggtt aaacccaacc cagccccgac aaatcccagt cccggccagg agaaaaggcc
      961 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     1021 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     1081 tgctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacgtctgt
     1141 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     1201 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     1261 tcgtcgatct cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     1321 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     1381 atcttttccc agccagctga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc
     1441 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgccagaaca acactttgta
     1501 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     1561 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     1621 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     1681 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     1741 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     1801 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     1861 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     1921 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc agacatagtt
     1981 acgctgaagt tgccctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     2041 cgtatccagg cggattaaat tcttttcctc ttgttcgccg ctggattgtt cggcaactaa
     2101 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gggcacttaa
     2161 tacaatatct gccggcaaac ctttgcgggc aaatgcgtca tcaatgcgtg agcgccccgt
     2221 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     2281 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccagc gaaaccacgg
     2341 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     2401 gccattttgc aacaacgtcg caatttcctg tggcgtaccc tggattagct cgagccgaac
     2461 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     2521 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     2581 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     2641 ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caagttcatc
     2701 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     2761 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     2821 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     2881 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     2941 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     3001 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     3061 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     3121 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     3181 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     3241 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     3301 acatcaacgc tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     3361 aaagcctgac tggatacccc agccacaggg gaatgctcat aaataaccgc catatcgagt
     3421 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     3481 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     3541 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     3601 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     3661 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     3721 ccttccagtg tggcaacctg ctggctgagc gctggttgtg cgatatgcaa tacttcagca
     3781 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     3841 atgttgcctc cggtttttaa gagtcggccc aagtgccgcc attacttaca accagaatgc
     3901 aagatgcttg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     3961 gattatgata gattcgtcat tggctctttt tatcagcagt cgcattcacc tgccctataa
     4021 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     4081 tgaaattggc agcaaacaaa caaaaaaatg catttcatcc tttgacatca ccatgcactg
     4141 ccattaatat gcgccccgtt cacacaattc ctctgtagtt cagtcggtag aacggcggac
     4201 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
     4261 ctttcgagca ggctttttac tttctaatta ccaacttatt ggtcattcca gatcagcatg
     4321 tcgggtaaac attgtggcac tgctttcacc cagttccgac atcaacgcca gaacaattgc
     4381 atcataagta ataaagcaga gttgctcaaa agctgagccc attggttgcg cgaaagtgcc
     4441 tgcaacacga ttgttatcgt ctttcacact tcctggtagt accaacacag attgagctaa
     4501 tttaccgatc gtcgaatcag ctttcatagt gaccagagcg acgtttactc cgctatcaac
     4561 tgctttttgt gccagacttt tcaaactggt agtttcacca ctgcctgacc caataattaa
     4621 tagatctcca ggttgggtat gcggtgatga tatttctccc acaacgctta ctgaaaaacc
     4681 taagtgcagc aagcgattag caaaagcgcg aatagcaatg ccactacgcc cagctccctg
     4741 caaaaaaata tgccgggtat ttctgatttg cgaaataaac tgtgcagcct gagaatcgtc
     4801 aatcttcagc gcattattct ctaattcatg cagaatatta atagtatttt cctgaacact
     4861 cattttttct ctcccttttt aagcaatagt ccggccacca tccattaaaa tggtttgtcc
     4921 ctgaatataa gagttatctt tatccgccaa aaatattgcc aggttagcca catcatcgat
     4981 agtccccaga cgacggacgg gtattttttc caggacagcg tctaaatcgg cttgtgttgg
     5041 ataattgacc cgcatcatct ctccggtgtc tattaagcga ggagcgatcg cattaacatt
     5101 gacgcctttt ggtcctaact cctttgccag accgcgaatc aacccttcac ctcccgcttt
     5161 gctggcagga taactcacac cgcccccagt ccccgaaagc gctgatcccg atgagatata
     5221 aacaatagat cccttatttg tcaggaaatc atcataaaac gccttaacgg tattaaataa
     5281 tcctgataag ttaattgcga tagtcttatt ccattcctca aaagaaatat tgttaacttt
     5341 attggcaaat gccacaccgg catttaatat caacacatca atacgaccaa aggtagcaat
     5401 taccttttcc cgtacatcga ctaatgcctg tggatcggag acatctgttt taacaaacag
     5461 cgatttaaca tcgccaaaag ttaactctgc tgcagtggcc ttgccgttct cttcgttata
     5521 gtcaacaata acagtatgtg caccacgatc agcaaattgt ttagctatcc ctttgccaat
     5581 accgtgagca cctccggtaa ctaaaatcac tttacctgca aaattactca ttacttttct
     5641 ccattaaaga ctttttagtt gaggttttcc tcccgatatg aaatcgaaaa cctcacttag
     5701 ctggatgtaa aacttttaga ttgtgatcac tgaacagcga tttttaatgc ctgataactt
     5761 tcacgtggct gtttgttaaa taatgcaccg ccaacaacaa taaggtcagc ccccgcttct
     5821 ttaacttgtt tcacattatc aagattaacg ccaccatcca cttcaatttg aatatctctg
     5881 ccagatatca tttttttggt attacggatt ttctccagac ttttatgaat aaatgtctgg
     5941 cctggttgcc cggggttaat tgtcattaac aatatataat caatatcatc taataaatac
     6001 tcgatgacag attcaggtgt cccaggatta agcacaacac ctgctttgcg ccccgctttt
     6061 ttgatttttt gaataatata atgtacatgt ggcgtagatt cataatgtac agaaatcatc
     6121 tccgccccgg tattgattac atcatcaata tgtcgctcag gatttgccag catcatatgc
     6181 acatcaaaga tcatcgagct ggcttttttc attgctgcaa tttgatttgg gccaaaggca
     6241 atattactga cgtaagtgcc atccattaaa tcaacgtgca atatttcagt ttgttcctct
     6301 tccagaaaat gaatttcatc tttcaggcgt aaaatatccg cgccaaatat agaaggtaat
     6361 atttttgcca tttaaaaact ccagattatt aaatcaatgt aatacggtgc tcaaccaata
     6421 ggttaaaatt tcccacgggc cggttaatac aaaatctgta ccttgtcctt gcaagcctac
     6481 accggctcca gctaaaaatt gcgttgcccc tggtgcgaca taaccgccta aatacatcac
     6541 tacaacgcag aataacaagg tcgagataat cgaacgaacc acgttaccat tgctgaccat
     6601 tacggtcatg gtggtcatat aaataattga catcaatacg ccaattggga agatcttaat
     6661 atcaggtaat accagagcac atagcatggt taaaggaatg gtaatgactg tcacggtgac
     6721 cgtcgccgga tcccccaatg ccagtgcaca atccataccg atgtttaatt cagcatcttt
     6781 acccatctgt ttttgcatga tttctttggc acttttccca atcgggttaa gaccttccat
     6841 cagcacaccg accactttag gcaataatac cattacgcca gcaaccccca tcgccatggg
     6901 aacaattgtg gtaatggcct gtttcgttaa aactccgagt aatgcgccaa cgataatacc
     6961 gataattgca ggttcaccaa agacacccag gcgtttttgc acgctgttta agtcaacatc
     7021 taacttattt aacccgggga tgtaatcaat gattttattt actacccagg cgaacggtag
     7081 agtccagccg atatgaatca tcgtggtaca ggtcgttccc tcgagtccat aatatttttg
     7141 ccatctgggc gcaatcaaat cgccaataaa cagagccgca atactcaaag ccaccgcgac
     7201 agagaaaccg atgataaaac tgtcgaacac aaaatatgcc agagcacccg gaactaaaaa
     7261 atgcatatag ttccagatat caacattcaa cgttttggtt aatttcaggc gcaccagaat
     7321 caggttaagt acgatgccaa ctggtatcac taatgcggca aaaggtgcta cccatgcagc
     7381 agcaccgact gctggccaac caatatctgc aaccgtaaaa ccactaccta cttttgagta
     7441 atattcaata gctggctgta atgaactatt taataaacca acaaccaacc ctaaaccaat
     7501 aaaaccaata ccgacggtga tgcccgactt gatcgcggca cctggtttca ttctgaacac
     7561 cagacctaaa ataaatatca ccagcggcag tattgcagtc gcccccattc cggtgattgt
     7621 ttccattaaa tttttcaaca tttccatttt tattattcct gtcaggttta gctatttgct
     7681 aattcagtta ataactctcc taatttcttg cgtaatgctc cttcattaat acccgttaca
     7741 aaacctgcaa cgctcatatg tggtttccca atatcacctt tatagttatt agttgtgagt
     7801 acaatatctg catgagccat ctcagaagaa agttcagcca tactgcattt gttgatttta
     7861 aatgccgtaa taccatattc agcacatact gacttcactt catcagcagc aagcgtagat
     7921 gtagcgacac cgctaccaca agcaacaaga atattaatca taataaactc cagtttttac
     7981 tattggatta ttttggacac aaaaagtgct tttgcatcat cgacgggaag tgtcagtaag
     8041 ttttggtaaa attcttcatt ttggagatgg ttaaataatg actttaacaa tcgaagctgg
     8101 tcagcaggat ccgtaacaat aagattaacg acgagtctga cccacaatat tccatcttca
     8161 tcagccctgt tcacaggtat tgcttctggc agacggatga tataaatagc cggttgactc
     8221 gcatgttcac tatcacagtg tggtatagcg atagaataac cttccagttc tattcctgtc
     8281 ggatattccg cttcgcgttt acgcatagcc ttgagccagc tctctttcac cgtattcgtt
     8341 tcacgatgcg tgttataaat atgttcaagc acggcatcgt agttctgtag tcccgcacca
     8401 tcggcaacac tgatcgatat catatccttt tcccctcacc ccacaatgct cacatgagca
     8461 ttaacaaatc ttcatatgag cagttataac acaacgatcc tgtggataaa taacgagatc
     8521 tgagaaatta aatccagatc acaaaaaagc acataatggc gatagttttt atgctcatat
     8581 gagcatggtg taattaaaaa atgatttctt cgcgaaaaag gaagtaaaac tgacaataag
     8641 tagaggacga attgaataga gatcgataag taagcatcac cagggatgct cacttgataa
     8701 ttgggaaatg attacatttg gctaagctga ttcgaaggga caaggcgtgt cgtagtaagt
     8761 tctagcaggt tattactgac gtgtattttg cgacttcggc aatcccctct ttgtcggcat
     8821 gatcgttggt aataagggcg gtgatctccg accagtcgca gatcttaaac aaactgctgc
     8881 gctcaaactt ggtgtaatct ccgaggatat attttttgtc tgagtgcgaa attatcgtgc
     8941 gatccaaaaa cgcatcagaa gaagaaggcg ttgatggacc agtcatgtta ctaaagccgt
     9001 cggttccaat gaaacagaga tcaacatgta tatcgttgat catagatacc gcccaccccc
     9061 caaacactga ggaactcttt cttcgcagtt caccgccaaa gagaaaaacc tggttgtcag
     9121 attcaatcaa cacactggca acggggagcg aatcagtgaa gattttaaac ccagacttca
     9181 tacataacag gttagccagc tcaaaagtgg tactgcccgt accgataatc atcgaactat
     9241 taggcggaat aaaatcaagc gctttacgag caatcgcctt cttataagag ttattctctt
     9301 tttccctgac ctgaaaggga tgctctatgg ccctttgctt taaagtcgcc ccaccatggc
     9361 ttttgatcag cagccctttt tgttcaagat gggtcaaatc tttcctgatg gtttcatacg
     9421 ttacttcata ctttcttgcg agatcactga catagaccgt acctgccgac attaagtctt
     9481 caagtattaa ctttcgcctt tcatcaacca gatacatcct tcctcctgaa atctattgcc
     9541 catctgagaa aacgccaagc atagcacaga tgatgcaaac aaaaacaaac actaaccgtg
     9601 gaaagtacaa ataagcccaa taaagacaat agttcaaaag ccatatatcc aattgataat
     9661 aaatgaattt ttattattca ttacaacgcc actgtacaat cccaatttgt ctcaattggc
     9721 ttaatttatt ttttatttgt ttttatttgt ttttaatatc ataaaaatca atcagttata
     9781 aattttgtga ttttgatctc aattgacaac caatatttac caacagcata cttggcttta
     9841 gttacttaac gacaggcaaa atgaaatgaa aacgaagatt gctatcgcaa gcgatgatgt
     9901 aggttttgca cgtaaagagg agataaaaaa atacctcata gaagagaaaa acatggagat
     9961 cgtgtacgat cccgttacat gtaaggaaca aggtgtcaat aacttcgcgc gtctggccga
    10021 cgaaatggca ggcattattc aacgcgatga gtgccgtctg ggaatttaca tttgtgggac
    10081 tggcattggc tttacttgtc agatcaacaa acactggggc ataagagccg ttgcggttac
    10141 taacccctac tccgcaaagc gggcgcgact cagcaataac gcacaagtta ttggactggg
    10201 ttgccgggtc aacgatctcg aatacagcaa aatgattgtt gatgcctggt tggataaccc
    10261 attcgacttc accaccgcca gagacaattc taaaaaaaac ttactggaag ctgaacgtaa
    10321 cgataatgca ttgctggtta agcccgctga tgtacgctgg aatatgggtt tccgaccaga
    10381 tgatgaaaaa tctgagggtt aatgtatgga actgatcacg caatttataa acgatctggg
    10441 aaattttata tttatcccgg tcatctttct ggtactgatg aagatacttg gccgtcctct
    10501 ttcagaatgt atctcctccg caatcaaagt cggcattggt ttcattgcgc taaccatgac
    10561 catcaaactg atgctggaaa aaatgcaacc ggcagtcacc ggattagcag aagcaacagg
    10621 ttcctcgctc agtgccatcg atgttggtgg cgcagcgact gcggttatgg gatttggctc
    10681 cagcatgggc gctatcatta ttcccctctg tgttgcggta aatattgcaa tgctggtcgc
    10741 ccgcctgact gactgtgtta acgttgatgt tttcaacctt catcaaaatg cgtcaatggg
    10801 ggcaattgtt ggcgtctatt ctggtagctt cctgtatggc gcattgaccg ccgcgctatt
    10861 ccatgtctgg gcgctgatcg ctgccgatct cggtgctaaa aataacgaaa aattctttaa
    10921 cctgccaaaa ggtgttgcga tctctcaccc ggttgccaat acctacttac ttttcgctta
    10981 tccattcaac tggatttatg atcgcatccc aggcttccgt aatctgaatg tgaccgccga
    11041 aactattcaa aaacggtttg gcattctggg cgatccaact atggttggtt ttattattgg
    11101 tattttgttg ggcttttgtg gttatggctg gaaatccccc taccacacca taatcgccag
    11161 cctacagtta gggatgtatc tggctgcggt catgcttctg ttgccacgta tgacctctat
    11221 catgatggaa gggcttgttc cgctttccaa cgtggcacgc aaaaaactgg tcaaacgttt
    11281 cccggatcgt caaatcactg ttggtatgga cactgctctg attgtgggcc atccatcagt
    11341 tatcgcccct gcattattac tgatcccggt cattgtgatc ctcgccgtga tcttgcccgg
    11401 caaccgcgtt atgccactgg gtgacctctc tcagtttgtg tttttcattg cctgcatggt
    11461 acctgttttc aatggcaaca ttattcgcac ctgggtgacc tcgatcattt tgttcggtgg
    11521 tggtctgtat attgcatcgt ggatggcacc ggctaccaac gaagtcttcc agaagtttgg
    11581 tacaaacccg gatgccagcg tgatgtactc ttcgcttaac ccgtctgcga atccatttac
    11641 tggtctgttt gccgccctga gccatgttgg aatcattggc tatgtgatgg caggtatcct
    11701 tttgttatct attggatact taattaaaca aaaatcacgt cgccagattg aaa
//
LOCUS       DAZKHK010000142        11629 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKHK010000142 DAZKHK010000000
VERSION     DAZKHK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.143"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(134..1495)
                     /gene="rhlE"
                     /locus_tag="ACHOS5_004210"
     CDS             complement(134..1495)
                     /gene="rhlE"
                     /locus_tag="ACHOS5_004210"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP9778934.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRTESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            1724..2395
                     /gene="cecR"
                     /locus_tag="ACHOS5_004211"
     CDS             1724..2395
                     /gene="cecR"
                     /locus_tag="ACHOS5_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP9778935.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2398..3393
                     /gene="hlyD"
                     /locus_tag="ACHOS5_004212"
     CDS             2398..3393
                     /gene="hlyD"
                     /locus_tag="ACHOS5_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP9778936.1"
                     /translation="MKKTVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     SLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLVAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPNKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3386..5122
                     /gene="ybhF"
                     /locus_tag="ACHOS5_004213"
     CDS             3386..5122
                     /gene="ybhF"
                     /locus_tag="ACHOS5_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9778937.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDAALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEIDINETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHDNE"
     gene            5115..6248
                     /gene="ybhS"
                     /locus_tag="ACHOS5_004214"
     CDS             5115..6248
                     /gene="ybhS"
                     /locus_tag="ACHOS5_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9778938.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRQLD"
     gene            6259..7365
                     /gene="ybhR"
                     /locus_tag="ACHOS5_004215"
     CDS             6259..7365
                     /gene="ybhR"
                     /locus_tag="ACHOS5_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9778939.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7327..7737)
                     /gene="ybhQ"
                     /locus_tag="ACHOS5_004216"
     CDS             complement(7327..7737)
                     /gene="ybhQ"
                     /locus_tag="ACHOS5_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP9778940.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRPPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7870..8631
                     /gene="ybhP"
                     /locus_tag="ACHOS5_004217"
     CDS             7870..8631
                     /gene="ybhP"
                     /locus_tag="ACHOS5_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9778941.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8628..9869
                     /gene="clsB"
                     /locus_tag="ACHOS5_004218"
     CDS             8628..9869
                     /gene="clsB"
                     /locus_tag="ACHOS5_004218"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP9778942.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            9869..10825
                     /gene="ybhN"
                     /locus_tag="ACHOS5_004219"
     CDS             9869..10825
                     /gene="ybhN"
                     /locus_tag="ACHOS5_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP9778943.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10861..11574)
                     /gene="ybhM"
                     /locus_tag="ACHOS5_004220"
     CDS             complement(10861..11574)
                     /gene="ybhM"
                     /locus_tag="ACHOS5_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9778944.1"
                     /translation="MESYSQNSNKLDFQHEARILNSIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSSVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         2688 a         3076 c         3149 g         2716 t
ORIGIN      
        1 gcgccttatc cggcctacaa aaacatgcac attcaatata ttacagagtt cacgtaggcc
       61 tgataagcgt agcgcatcag gcagttttgt gtttgtcatc agtctcatgc cgggcatagc
      121 ccggcataaa agattactgc gcagcggcag gtttacgcgg acggcggcga cgttgttgtt
      181 cgcctgccgg tttggcatcg ccgagacgac gagacggttt ttctgcgggc ttcgcgttac
      241 cagacttcgc accactttcc gtacggcgtg gttgctgttg accgcgacca ccgccctgtc
      301 cacgaccgcc gccacgttgc tggcgaccgt tctggatcgg ttcggctttg attgacgggt
      361 ccggctcata ccccggaatc gcaatgcgcg ggatctcttt tttcagcagt ttttcgatat
      421 cacgcagcag tttgtgttca tcaacacaca ccagcgacaa tgcttcgcca gttgccgccg
      481 cacggccagt acgcccgata cggtgaacat aatcttccgg tacgtttggt aactcatagt
      541 tgacgacgtg cggcaactct tcgatatcca gaccgcgcgc agcgatgtca gttgccacca
      601 gtacacgaat atcgcccgat ttaaaatcgg ccagcgcacg agtacgcgca ccttgcgatt
      661 tattaccgtg gatcgccgca ctacggatac cgtctttatt aagctgttca gccagatggt
      721 tagcgccatg tttggtacgg gtaaacacca gcacctgctg ccagttccct ttcccaatca
      781 tatgcgacag caattcgcgt ttgcgtttct tatcgacaaa gtgaacgtgc tgggtcacct
      841 gatcagacgc agtattgcgg cgtgccactt cgatttccag cgggttgtgc aacagttttt
      901 ccgccagggc tttaatatcg tcagagaagg tcgcggagaa caacaggttc tggcgcttcg
      961 ctggcagttt tgttaacacg cgacggatat cgtggataaa gcccatgtcg agcatgcggt
     1021 cagcttcatc aaggacgagg atttcaacct gatccagctt cactgcattt tgatgttcca
     1081 ggtccagcag acgtcccggc gttgccacca gcacatcaac gccgccgcgc aacttcatca
     1141 tttgtgggtt aatgctgaca ccgccaaaca ccaccagcga acgaatgttc agatatttgc
     1201 tgtaatcacg gacgttttcg ccaatctgcg ccgccagttc acgggtcggg gtaagaatga
     1261 gcgcgcgtac cggacgacgc cctttggcgt gcggctggcg agtgatcagg tgttgcaaca
     1321 gcggcagcgt aaagcccgct gttttgccgg taccggtctg ggcgctggcc atcaggtcgc
     1381 ggccttccag caccgcaggg atcgcctgct gctgaatagg ggtgggttca cggtaaccct
     1441 gctcggcaac ggcgcgcagg atatcagggc ttaaacccaa agaatcgaaa gacataacta
     1501 ctccgaaccg ccccgaccgt taccggtgta gttttcaggg agatactgaa aaggaaaatg
     1561 acaaaaacca caatgtcatg agggagcgga gtgtagcagt ttttgtgacg cagcgcataa
     1621 attatccctg tgactggcgt agctaaaatt cttagccata ctggacaact cccctatgga
     1681 gtcataatct taatcaatca tttgattaag aatggactgt gcgatgaata atcctgccat
     1741 gacaatcaag ggtgaacagg cgaaaaaaca gctgattgct gccgcactgg cgcagtttgg
     1801 tgaatatgga atgaacgcca ccacccgcga gatagccgcc caggccgggc agaatatcgc
     1861 tgccatcacc tactacttcg gttcgaaaga ggatttgtac ctcgcctgcg cccagtggat
     1921 tgccgatttt attggcgagc agttccgtcc gcacgccgaa gaagccgaac gtttgttcgc
     1981 gcaaccacag cctgatcgcg cagctatccg tgaactgatc cttcgcgcct gcaggaacat
     2041 gattaagctg ctcacccagg atgacaccgt caatctcagc aagtttatct cccgtgagca
     2101 gctatctccc acggcagcct accacctggt gcacgaacag gtgattagtc cgctacacag
     2161 ccaccttaca cgcctgattg ccgcctggac cggctgcgac gccaatgata cccgcatgat
     2221 ccttcatacc catgcgttga ttggtgagat tctggcgttt cgtcttggca aagaaacaat
     2281 tctgttacgt accggctgga ccgcgttcga tgaagaaaag accgaactga tcaaccagac
     2341 ggtgacctgt catatcgacc tgattttgca aggattatcg caaaggagtt tgtagtgatg
     2401 aaaaaaactg tcgtgatcgg actggcggta gtggtacttg ccgccgtggt tgccggaggc
     2461 tactggtggt atcaaagccg ccaggataac ggcctgacgc tgtatggcaa cgtggatatt
     2521 cgtacggtga gtcttagttt ccgtgttggg gggcgcgttg agtcgctggc ggtggacgaa
     2581 ggtgatgcga tcaaagcggg ccaggtgctg ggcgaactgg atcacaagcc gtatgagatt
     2641 gccctgatgc aggcgaaagc gggcgtttcg gtggcgcagg cgcagtatga cctgatgctt
     2701 gccgggtatc gcgacgaaga aatcgctcag gccgccgcag cggtgaaaca ggcgcaagct
     2761 gcctatgact atgcgcagaa cttctataac cgccagcaag gactgtggaa aagccgcacc
     2821 atttcggcaa atgacctgga aaatgcccgc tcctcgcgcg accaggcgca ggccacgctg
     2881 aaatcagcgc aggataaatt gcgtcagtac cgttccggta accgcgaaca ggacatcgcc
     2941 caggcgaaag ccagcctcga acaagcgcag gcgcaactgg cgcaggcgga gctgaatttg
     3001 caggattcga cgctggtcgc tccgtctgat ggcacgctgt taacgcgcgc ggtagagcca
     3061 ggcacggtgc ttaatgaagg tggcacggta tttaccgtct cgctgacgcg tccggtgtgg
     3121 gtacgcgcct atgttgatga acgtaatctt gaccaggccc agccggggcg caaagtgctg
     3181 ctttataccg atggtcgtcc gaacaaaccg tatcacgggc agattggttt cgtttcgccg
     3241 actgctgaat ttaccccgaa aaccgtcgaa acaccggatc tgcgtaccga cctcgtctat
     3301 cgcctgcgta ttgtggtgac cgacgccgat gatgcgttac gccagggaat gccggtaacg
     3361 gtgcaattcg gtgacgaggc aggacatgaa tgatgccgtt atcacgctga acggcctgga
     3421 aaaacgcttt ccgggcatgg acaagcccgc cgtcgcaccg ctcgactgca ccattcacgc
     3481 cggttatgtg acggggctgg ttgggccgga cggtgcgggc aaaaccacgc tgatgcggat
     3541 gctggcggga ttactgaaac ccgacagcgg cagtgccacg gtgattggct ttgatccgat
     3601 caaaaatgac gctgcgctgc acgctgtgct cggctatatg ccgcagaaat tcggcctgta
     3661 tgaagatctg acggtgatgg aaaatctcaa tctgtacgcg gatttgcgca gcgtcaccgg
     3721 tgaagcacgg aagcagactt ttgctcgcct gctggagttt acatctcttg gcccgtttac
     3781 cggacgcctg gcgggcaaac tctccggtgg gatgaaacaa aaactcggcc tggcctgtac
     3841 cctggtaggc gaaccgaaag tgttgctgct cgatgaaccc ggcgtcggcg ttgaccctat
     3901 ctcacggcgc gaactgtggc agatggtgca tgagctggcg ggcgaaggga tgttaatcct
     3961 ctggagtacc tcgtatctcg acgaagccga gcagtgccgt gacgtgttac tgatgaacga
     4021 aggcgagctg ctgtatcagg gagaaccgac ggccctgact caaaccatgg ccggacgcag
     4081 ctttctgatg accagcccgc acgagggcaa ccgcaaactg ttgcaacggg cattgaaact
     4141 gccgcaggtc agcgacggca tgattcaggg gaaatcggta cgtctgatcc tcaaaaaaga
     4201 gaccacgcca gacgatattc gccatgccga cgggatgccg gaaatcgaca tcaacgaaac
     4261 tacgccgcgt tttgaagatg cgtttattga tttgctgggc ggtgccggaa cctcggaatc
     4321 gccgctgggc gcaatattac atacggtaga aggcacaccc ggcgagacgg tgatcgaagc
     4381 gaaggaacta accaagaaat ttggtgattt tgccgccacc gatcacgtca actttgccgt
     4441 taaacgcggg gagatttttg gtttgctcgg accaaacggc gcgggtaaat cgaccacctt
     4501 taagatgatg tgcggtttgc tggtaccgac ctctggtcag gcgctggtgc tgggcatgga
     4561 tctgaaagag agttctggta aagcgcgcca gcatctcggc tatatggcgc aaaaattttc
     4621 gctctacggc aacctgacgg tcgaacagaa tttacgcttt ttctctggtg tgtatggctt
     4681 acgcggtcgg gcgcagaacg aaaaaatctc ccgtatgagc gaggcgttcg gcctgaaaag
     4741 tatcgcctcc cacgcgaccg atgaactgcc attaggtttt aaacagcggc tggcgctggc
     4801 ctgttcgctg atgcatgaac cggacattct gtttctcgac gaaccgacgt ccggcgttga
     4861 tcccctcacc cgccgtgaat tttggctaca tatcaacagt atggtagaga aaggcgtcac
     4921 ggtgatggtc accacccact ttatggatga agcggaatat tgcgaccgca tcggcctggt
     4981 gtatcgcggg aagctgattg ccagcggcac gccggacgat ttgaaagcac agtcggccaa
     5041 cgatgagcaa cccgatccca ccatggagca ggcctttatt cagttgatcc acgactggga
     5101 taaggagcat gacaatgagt aatccgatcc tgtcctggcg tcgcgtgcgg gcactgtgcg
     5161 tcaaagagac gcggcagatc gttcgcgatc cgagtagctg gctgattgcg gtggtgatcc
     5221 cgctgctact gctgtttatt tttggttacg gcattaacct cgactccagc aagctgcggg
     5281 tcgggatttt actggaacag cgtagcgaag cggcgctgga tttcacccac actatgaccg
     5341 gttcgcccta catcgacgcc accatcagcg ataaccgtca ggaactgatc gccaaaatgc
     5401 aggcggggaa aattcgcggt ctggtggtta ttccggtgga ttttgcggaa cagatggagc
     5461 gcgccaacgc caccgcaccg attcaggtga tcaccgacgg cagtgagccg aataccgcta
     5521 actttgtaca ggggtatgtc gaagggatct ggcagatctg gcaaatgcag cgagcggagg
     5581 acaacgggca gacttttgag ccgcttattg atgtacaaac ccgctactgg tttaacccgg
     5641 cggcaataag ccagcacttt attatccccg gtgcggtgac cattatcatg acggtcatcg
     5701 gcgcgatcct cacctcgctg gtggtggcgc gagaatggga acgcggcacc atggaggctt
     5761 tgctctctac ggagattacc cgcacggagc tgctgctgtg taagctgatc ccttattact
     5821 ttctcgggat gctggcgatg ttgctgtgta tgctggtgtc ggtgtttatt ctcggcgtgc
     5881 cgtatcgcgg gtcgctgctg attctgtttt ttatctccag cctgttttta ctcagtaccc
     5941 tggggatggg gctgctgatt tccacgatta cccgcaacca gttcaacgcc gctcaggtgg
     6001 ccctgaacgc ggcttttctg ccgtcgatta tgctttccgg ctttatattt cagatagaca
     6061 gtatgcccgc ggtgatccgc gcggtgacgt acattattcc cgcacgttat ttcgtcagca
     6121 ccctgcaaag cctgttcctc gccgggaata ttccagtggt gctgatagtg aacgtgctgt
     6181 ttttgatcgc ttcggcggtg atgtttatcg gcctgacgtg gctgaaaacc aaacgtcagc
     6241 tggattaggg agaagagcat gtttcatcgc ttatggacgt taatccgcaa agagttgcag
     6301 tcgctgctgc gcgaaccgca aacccgcgca attctgattt tacccgtgct gattcaggtg
     6361 atcctgttcc cgttcgccgc cacgctggaa gtgaccaacg ccaccatcgc catctacgat
     6421 gaagataacg gcgagcactc ggtagaactt actcaacgtt ttgcccgcgc cagcgccttt
     6481 acccatgtgc tgctgctgaa aagcccgcag gagatccgcc cgaccatcga cacacaaaag
     6541 gcgttactac tggtgcgttt cccagctgac ttctcgcgca aactggatac cttccagacc
     6601 gcgccgttgc agttgatcct cgacgggcgt aactccaaca gtgcgcaaat tgccgccaac
     6661 tacctgcaac agatcgtcaa aaattatcag caggagctgc tggaaggaaa accgaaaccc
     6721 aacaacagcg agctggtggt acgcaactgg tataacccga atctcgacta caaatggttt
     6781 gtggtgccgt cgctgatcgc catgatcacc actatcggcg taatgatcgt cacttcactt
     6841 tccgtcgccc gcgaacgtga acaaggtacg ctcgatcagt tgctggtttc gcccctcacc
     6901 acctggcaga tattcatcgg caaagccgta ccggcattaa ttgtcgccac cttccaggcc
     6961 accattgtgc tggcgattgg tatctgggcg tatcaaattc ccttcgccgg atcgctggcg
     7021 ctgttctact ttacgatggt gatttacggt ttatcactgg tgggattcgg tctgttgatt
     7081 tcatcgctct gttcaacgca acagcaggca tttatcggcg tatttgtctt tatgatgccc
     7141 gccattctcc tttccggtta cgtttcgccg gtggaaaaca tgccagtgtg gctgcaaaat
     7201 ctgacgtgga ttaaccctat tcgccacttt acggacatta ccaagcagat ttatttgaag
     7261 gatgcgagtc tggatattgt gtggaatagt ttgtggccgc tactggtgat aacggccacg
     7321 acagggtcag cggcgtacgc gatgtttaga cgtaaggtga tgtaacttct tatctttcgc
     7381 cagcaaagac accaccgccg ggccggcaag gattgccagc cctgcaattg ccagcaaaaa
     7441 gttgttttcc agtacgcggg acaacagcca cagcacaatc agcgctgcac caaaatacca
     7501 ggtcgtggtc agctcttcca gcacgtctgc cacctcacgc cagcgttgct cgggggggcg
     7561 ctgaaacacc agcatcatca ctaccgtgac acaatacagt gcgcataatc ctacgccgac
     7621 gtgaaccaga gttttactca tccagcccac caccagcagc gccactacca gtaaatgcaa
     7681 cataatctgc cagcaactta ggccagttgc gacacgaaca cgttgttgcc acttcatggc
     7741 ttctctcctg aagaatttat ctctacttat tcagagtacc gaactttacg gaaaattccg
     7801 taagaccgca cctttttgtg acgccgacta cactatttca atgagataag aggcaaagac
     7861 aggaaaatta tgcccgatca aacacaacaa ttttcgttca aggtgctcac catcaatatt
     7921 cacaaaggct ttaccgcgtt taaccgacgc ttcattttgc cggaacttcg cgacgccgtg
     7981 cgtaccgtca gcgccgatat tgtttgcctg caggaagtga tgggcgcgca cgaagttcat
     8041 ccgctgcatg tggaaaactg gcccgatacc tcgcactacg agtttctcgc cgacaccatg
     8101 tggagcgatt tcgcctacgg tcgcaatgcc gtatacccgg aaggacatca cggtaacgcc
     8161 gttctttcac gttatcccat tgaacattat gagaatcgtg atgtttcggt cgatggtgcg
     8221 gaaaagcgcg gcgtgctcta ctgccgcatt gtgccgccga tgaccgggaa agcgattcat
     8281 gtgatgtgcg tacatctggg cctgcgtgag gcgcaccgtc aggcgcagct tgcgatgctc
     8341 gccgaatggg tgaatgagct accggacggc gaaccggtat tggtagcggg tgatttcaac
     8401 gactggcggc aaaaagctaa tcatccgtta aaagtgcagg ccgggctgga tgagattttt
     8461 acccgcgccc acggacgccc ggcgcgcacg tttccggtgc aatttcctct actacgactg
     8521 gacaggatct acgtcaaaaa tgccagcgcc agcgcgccaa ccgcgttgcc gctgcggaca
     8581 tggcgacacc tttctgatca tgccccttta agtgcggaga ttcatttatg aaatgtagct
     8641 ggcgcgaagg caataagatc cagttgctgg aaaacggcga gcaatattat cccgcggtgt
     8701 ttaaggcgat tggagaggca caagaacgca tcattcttga aacgtttatc tggtttgagg
     8761 atgacgtcgg caaacagctg catgcggcgc tattggcagc agcgcaacgc ggggtaaaag
     8821 cggaagtctt gctggatggc tacggttcgc cggatctcag cgatgagttt gtcaatgaac
     8881 tgacggcagc tggcgtggtg ttccgctact acgatccccg ccctcgcctt tttggtatgc
     8941 gcaccaatgt gtttcgccgg atgcatcgca aaattgtggt gatcgacgcg cgtatcgcct
     9001 ttattggcgg gctgaattac tccgccgagc atatgtccag ctacggcccg gaggccaaac
     9061 aggattacgc ggttcgcctc gaagggccga ttgtcgaaga tattctccag tttgagctgg
     9121 aaaacctgcc aggacagagc gcggcgcgac gctggtggcg acgtcatcat aaagcggaag
     9181 agaaccgcca gccgggagaa gcgcaggtat tgctggtctg gcgcgataac gaagaacatc
     9241 gcgatgatat cgaacgtcac tatctgaaaa tgctcactca ggcgcggcgg gaagtgatta
     9301 tcgccaacgc ctacttcttc cccggctatc gttttttaca cgccttgcgt aaagcggcac
     9361 ggcgcggggt gcggatcaaa ctgatcattc agggcgaacc ggatatgccg attgtcagag
     9421 tcggcgcacg tttgctgtat aactatctgg ttaaaggcgg cgttcaggtg tttgagtacc
     9481 gccgccgccc gctccacggc aaagtggcat tgatggacga tcactgggcg acagtagggt
     9541 ccagtaatct cgatccgctc agtttgtcac tgaatctcga agcaaatgtc atcatccacg
     9601 atcgtaattt taaccagacg ctgcgcgata atctgaacgg cattatcgcc gcagattgtc
     9661 agcaggtcga tgagaccatg ctgccgaaac gcacctggtg gaacctgacc aaaagcgtgc
     9721 tggcgttcca ctttttacgc cacttcccgg cgctggtggg ctggcttccg gcgcacacgc
     9781 cacgtctggc gcaggttggt ccccccgcac aaccgacaat ggaaacgcag gatcgggtag
     9841 aaactgaaaa cacgggggta aaaccctgat gagtaaatca cacccgcgct ggcgcttagc
     9901 aaagaaactc ctcacctggc tgttttttat cgcggtgatt gtgttgctgg tggtctacgc
     9961 caaaaaagtg gactgggaag aggtctggaa ggtcatccgc gactacaatc gcgttgcgct
    10021 gcttagtgcg gtcgggctgg tggtcgtcag ttatctgatt tacggttgct acgacctgct
    10081 cgcccgtttc tactgcggtc acaaactggc gaagcgccag gtgatgctgg tgtcgtttat
    10141 ctgctacgcc ttcaacctga cactcagtac ctgggtcggc ggcattggta tgcgctatcg
    10201 tttgtactcc cgactggggt taccgggcag cactattacg cggatttttt cgctcagtat
    10261 taccaccaac tggctaggct atattttgct agcgggggtt atctttaccg caggcgtggt
    10321 ggagttgccg gatcactggt atgtcgatca aactacgctg cgtattctcg gtattggctt
    10381 actgatgatt atcgcggttt atttgtggtt ttgcgctttc gcgaagcacc gccatatgac
    10441 catcaaaggg caaaagctgg tgctgccttc gtggaaattc gccctcgccc agatgctgat
    10501 ttccagcgtt aactggatgg taatgggagc gattatctgg ctgttacttg gtcaaagcgt
    10561 gaactatttc tttgtactgg gcgtgttgct ggttagtagt attgctggcg tcatcgtgca
    10621 tattccggcg ggaattggtg tgctggaagc ggtgtttatc gcgctactgg ctggggagca
    10681 tacctccaaa ggcacaatta tcgccgccct gctcgcttac cgtgtgctgt attactttat
    10741 cccactgctg ctggcgctga tctgttacct gttgctggaa agccaggcga agaagctacg
    10801 ggcgaaaaat gaagcggcga tgtgaagtat agttaagttc tttagtatgt gcatttacgg
    10861 ctaatgaaaa aaccgcgtat gcctttgcca gacaagcgtt atagctatag cggcaagaat
    10921 cccaaagaag ccgataaaat agtaatagag tgttaatgca cctataaaag cacaacgaac
    10981 cgaactccat aattcttcac tatggctttt tcctgtcagt tcaaggagct tatttcgtcc
    11041 atgcgagata atgcctgacc ataagacaat cattaagcaa cttattaccc aaatgggtcg
    11101 ttcactcatt aatgtggcat tcacgatgat taccagggcc aaccctgtca acgtcatcat
    11161 aatgataaaa cggtgagaac caggatcaaa tttaaacaat agacctgcca gcatactgat
    11221 taaaaacatc gatcccgcag tgctgaacac gacgataatg gcagatacag gcgctaaact
    11281 cgcaacgaca atcgccacca aagagattaa aaacaaatat attacgcccg cgatggcggt
    11341 atttatcttg ttgatatctt tacttaagcc atacatagaa agtaataata atcctatagc
    11401 aacatacatt tgcggtgaat catattttgt tgccgtaatt tcgagatact tagcgcccca
    11461 ggccagccct gcggttgcca ccaaacccaa agcggtaatg agccatatgc tgtttaatat
    11521 cctggcttcg tgctgaaaat ctaatttatt gctattttgc gagtatgatt ccattctttt
    11581 agttgtcctt agaaaaaaca ttaatattca ccatcaataa atttaaaac
//
LOCUS       DAZKHK010000143        11601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKHK010000143 DAZKHK010000000
VERSION     DAZKHK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.144"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..355
                     /locus_tag="ACHOS5_004221"
     CDS             <1..355
                     /locus_tag="ACHOS5_004221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778945.1"
                     /translation="YRYDEFGRAVEKEGPQGTVVTVKSKQEAHDLLMEAFPDAQKVRG
                     IGSQDAAGIRKKHKMEQFKKRDGTVRYRKDYPIDSNTGRVYGHDDPKGTGHGSLPHIN
                     IKRSDGTMVRIDIDG"
     gene            348..650
                     /locus_tag="ACHOS5_004222"
     CDS             348..650
                     /locus_tag="ACHOS5_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9778946.1"
                     /translation="MVDLPIEKLIVAIENGKVRGFDEIKEINGENYFFQYAIKKQDEK
                     YNTYFFKIPENKMEVFEDYATEEISAFSNIEDAFNCFKLQGIDITKFSPIRGLLPF"
     gene            complement(684..1253)
                     /locus_tag="ACHOS5_004223"
     CDS             complement(684..1253)
                     /locus_tag="ACHOS5_004223"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9778947.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            1428..2273
                     /gene="nhoA"
                     /locus_tag="ACHOS5_004224"
     CDS             1428..2273
                     /gene="nhoA"
                     /locus_tag="ACHOS5_004224"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP9778948.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            complement(2369..3262)
                     /locus_tag="ACHOS5_004225"
     CDS             complement(2369..3262)
                     /locus_tag="ACHOS5_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP9778949.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLTGETRAAIINALHLTEDDILQGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLSHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(3342..4022)
                     /gene="narI"
                     /locus_tag="ACHOS5_004226"
     CDS             complement(3342..4022)
                     /gene="narI"
                     /locus_tag="ACHOS5_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9778950.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRVASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLNGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(4019..4714)
                     /gene="narW"
                     /locus_tag="ACHOS5_004227"
     CDS             complement(4019..4714)
                     /gene="narW"
                     /locus_tag="ACHOS5_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9778951.1"
                     /translation="MQILKVIGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRNRGQAMVDLLAEYENVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQICSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            complement(4714..6258)
                     /gene="narH"
                     /locus_tag="ACHOS5_004228"
     CDS             complement(4714..6258)
                     /gene="narH"
                     /locus_tag="ACHOS5_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9778952.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYDHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            complement(6255..9995)
                     /gene="narZ"
                     /locus_tag="ACHOS5_004229"
     CDS             complement(6255..9995)
                     /gene="narZ"
                     /locus_tag="ACHOS5_004229"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP9778953.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGANVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDSSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     QELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            complement(10090..11478)
                     /gene="narU"
                     /locus_tag="ACHOS5_004230"
     CDS             complement(10090..11478)
                     /gene="narU"
                     /locus_tag="ACHOS5_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP9778954.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         2846 a         2978 c         3019 g         2758 t
ORIGIN      
        1 ctaccgttat gacgaatttg gccgtgcggt ggaaaaggag gggcctcaag gaaccgttgt
       61 cacagttaaa tcaaaacagg aagcccatga tttattgatg gaggcctttc ctgatgctca
      121 aaaagtaaga gggatcggtt cgcaggatgc cgccggtatt agaaaaaaac ataagatgga
      181 acaatttaag aaaagagatg gtactgtccg ttataggaaa gattacccta ttgattctaa
      241 tactggtcgt gtatatggtc atgatgaccc taaaggcaca gggcatggtt ctttgcctca
      301 tataaatatt aaacgttctg atgggacaat ggtgaggata gatatagatg gttgatttgc
      361 ctattgaaaa attaattgtt gctatagaga atggtaaggt tagaggtttt gatgagataa
      421 aggaaataaa tggtgaaaat tattttttcc aatatgccat taaaaaacag gatgaaaaat
      481 acaataccta ttttttcaaa ataccagaaa ataaaatgga agtgttcgaa gattatgcaa
      541 ctgaagaaat tagtgcgttt tccaatatag aagatgcttt taattgcttt aaattacaag
      601 gtattgatat tacaaaattt tctcctatta gaggattatt acctttttag atgataaaga
      661 aacccggtta cagcatgtaa tgcttaacct gccgtaactc gcttaccact ggtaacaaac
      721 gtgccagcac ctaaatgatg caacgtattg agcttatcat catcaaactg ccagcggcct
      781 tcgacaaata cccgtgcgtc tgccgctgct gataccactt cgccaaatag cgtatcgtat
      841 ttttcttgcg cggaagttgc aggtagcagg cgacactcca tccacgccag acatttttcc
      901 tcgaccagcg gcaaaccaag caccgggcct ctcactaccg gaatgccgta gcaattaaat
      961 ttatcttctt cacgacccga cacacttccc accgcccacg tccagttagt cgccgcaacg
     1021 cccgggataa cgatgccaaa cttaccgtta cgttcaatca actccctggt ccatgtcgat
     1081 ttatctacca caatcgccac gcgtggcggt tcaaactcta cgggcatcga ccaggctgct
     1141 gccataatgt tacgccgctg ggattgctca tcgaaactgg tgatcatgac agttgggcca
     1201 tgatttaaca gacggctggc atggtgtaat tcgacgggaa tgaattggct cacgatatcg
     1261 cctcgctatg aagggtgttt taaaagcgta gctcgtaacg caataagtaa cgaaattaac
     1321 gggattggcg atttgcgaac gtgatgcatg tccgcgatcg cacaaaatag ccagtgcggc
     1381 gtctattcca ggttataagt tgagaaaacc actaagggaa acgcctgatg acgcccattc
     1441 tgaatcatta ttttgcccgt attaactggt tgggagctgc tgcggccaat attgatacgc
     1501 tgcgtgcatt gcacctgaaa cacaattgca ccattccatt tgaaaacctc gacgttttgc
     1561 tgccgaggga aatacagctt gatgatcaat cgctggaaga taaactggtg atagcccgtc
     1621 gtggcggtta ctgttttgag cagaatggcg tgtttgagcg ggtgttacgc gagctggggt
     1681 ttaacgttcg cagcttgtta ggtcgcgtag tgttatcaaa tccgccagca ttaccgccgc
     1741 gcacccatcg tttgctgttg gtggaactgg aaggggaaaa atggattgct gatgtcggtt
     1801 tcggtgggca gacgctaacc gcgccgattc gtttaatgcc ggatatcatg caaaccacgc
     1861 cacacggaga gtatcggttg ttgcaagagg gtgatgattg ggtattgcag tttaatcatc
     1921 atcagcactg gcagtcgatg taccgttttg atctctgcga gcagcaacaa agcgattatg
     1981 tgatgggcaa tttctggtcg gcgcactggc cgcagtcgca ttttcgccat catttgctga
     2041 tgtgccgcca tttgccggac ggtggcaagc taacgctgac caattttcat tttacccatt
     2101 atgaaaatgg gcacgcagtt gagcagcgaa atctaccaga tgtggcgtcg ttatatgccg
     2161 tgatgcagga acagtttggt ctgggcgtag atgatgcgaa acatggcttt actgtggatg
     2221 agttagcgct ggtgatggcg gcgtttgata cgcacccgga ggcgggaaaa taatttatgt
     2281 caggttgccg gatgtggcgt aaacgcctta tccggcatac attagcccgg ttgtagacct
     2341 gataagcgaa gcgcatcagg caaaggtttc agagttcaat tgcccattcg gcatgaaaca
     2401 aaatcaccgc cgtgccggaa atggtaactt tctccggttg gttatctcga attgtcaccg
     2461 tcacatcaat tacaccatca cgccccaatg cgcgcccctg atggccttta acacgcaaca
     2521 cgttgccatc gtgggataat acgttgtgat gtactaacca tgctcccatc ggaccattgg
     2581 cattcccggt caccggatct tccacaatac caatcgcagg cgagaacatg cgaccatcgg
     2641 tttcgttttt gcctgggcgg atctggaacg ggaagaaacc attgcagcca atctgcttgc
     2701 tgattgcggt cagcgcagca aggtcaggcg aaagggcgtc gatatccact tccggcttca
     2761 gtggaatcat cacttttgag tgtccggtgg tagccacctg gattggcaag ccttgcaaaa
     2821 tatcgtcttc agtgagatga agcgcgttga taattgccgc tcgtgtttca cctgtcagtg
     2881 gtggctcaaa gcctggagta ccttgttcca gcgaaatacg ataatcatca ttttgctttt
     2941 cgatagtcac acgatgtttt cctgccagcg atgtttgcca gaccgtgcaa tttcctaaac
     3001 ctaacacctt tgcacgtaca tagtgcgcag ctactgtcgc gtgaccgcaa attggcactt
     3061 caaccgttgg cgtaaagtag cggatgcgca catcgctgtc gtcgctgtgt agcagaaaag
     3121 cagtttccga atgacctaac tcgcgggcga taagctgcat ttgcgcttca ctgagattat
     3181 cggcggggaa aacaacaccg gcagaattgc cgcgaaatgg ttgagaggta aaagcatcga
     3241 cgtggtaaac ctgcggtttc attaatgttc tcctgtggct ggagaactat catagcctgc
     3301 aagtggccgg agagcgaagg gctatccggc ccgcgtgaga attaacgccg cgaacgcaca
     3361 atctgatacc gacgagtaaa gtactcaaac ggcgcgctcc acacatgcac cagtcgggtg
     3421 aacgggaaga gcaggaagat ggtcattcca agcaccagat gaacgcggaa tataaaggcg
     3481 acaccattaa gatgcgtaga tgcaccgccc tggaaagtga caatgctttg cgcccagccc
     3541 accagcttca tcatttcact gccgtccgga tgttgtgctg aaaaaggaat ggtggttaac
     3601 ccaagaatgc actgaattaa caaaatggcg aggatcagga tatccgcacc agaagacgtc
     3661 gccctgatac gcggatttgt taaacgacgc accagtagcc ccgcaccgcc aatcagcgtc
     3721 aataccccgc atattccgcc gaggatcatc gccatctgct gttttaccgc caccggcaga
     3781 aaccacgcgt acatccagtg cggcgttaac atgccgaaca gatgaccgaa gaagatcccc
     3841 agaatgccga tatggaacaa gttcgaccat agcaccatcc cgcgtttatc cagcatctgg
     3901 ctggaggcga cgcgccaagt gtactgcccg tagtcgtagc gcagccagct tccaaggata
     3961 aaaaccgtgc cacacaagta cgggtagata tcgtaaaaaa agacgttcag atactgaatc
     4021 atttcccacc tcccgcactg atgtcgacat actgcggcgc gacatcctgg ctaaagcgtc
     4081 gctgatattg attaagcggc gagctgtcac acgccgtggc gttatcttca ataaacttca
     4141 cctgttcttc ttcccacacc gcatccagcg cctggcgggt gtcgtcacgc tcttcactgc
     4201 aaatctgttt tgtgacactg tcacttgaca gagggcttcc tgccagttgc aacagggcgt
     4261 caaataacgc gtaccatggt gcctcgcgtt gttttaagcg tccgccaagc agggcgagga
     4321 tcggcgcaac gttgagtaat ccttctttcg cctgatcgtc cggaagtacg cttaaatact
     4381 ccagatacag cggtagataa tcgggcagtt ccctacaatt cagttgcaga ccgacgtttt
     4441 catactccgc cagcaggtcc accattgcct ggccgcgatt gcgggactcg gcatgaacat
     4501 gttcaaacag cagcagcgac gtggtgcgcc cgcggtcaaa cacttcacac cattcggcct
     4561 gtttatccag cagcggcgcg ttgagcaggt cgagtgcaaa atcggtgagc atcggcgcgt
     4621 cgcggcggat aagcgccagc gcgtcgtcct tacactccca caacaactcg tctgggtact
     4681 ccatcaacag gccgatcact ttgaggatct gcattattcg ccctccgctt tgtcgcgcac
     4741 ttcggtgatg ttgatggcat cgatacggct actgttgaac aggttgaatt tgctgtccga
     4801 accgtggcaa ccgtcgccaa aggtaaaacc gcagccgttg cgttctgcga aggcatcgcc
     4861 cgccatttcc cgatggcttg tcgggatgac aaaacggtct tcatagttgg caatggcgag
     4921 gtaacgatac atctcttcga cctgggcgac gctcaggcct acttcgtcga tggcacgagt
     4981 atcagtaacg ccttccacgg tttgtgaacg catatagtgg cgcatcgcca tcatccgttt
     5041 cagcgcccgc agtaccggac cggtatcacc ggcactcaac atattggcga gatattgcac
     5101 cggaatacgc aggctttcga tggcgggcag cacgccttcg cttttcggca agccgcccgc
     5161 atcagcgtag gactgaatcg gtgacagcgg aggaacgtac cagaccatcg gcagggtgcg
     5221 gtattcaggg tgcagcggta gcgccagttt ccagtccatc gccattttgt agacaggcga
     5281 acgctgggca gcgtcaatca cgttttgtgg aataccttgt ttcagggctt cttcgatcac
     5341 tgagggatcg tgcggatcga ggaacacttc gcactggcgt tcatagaggt caacttcgcg
     5401 ctcggtgctc gccgcttcct cgatgcggtc ggcgtcgtaa agcagcacgc ccagataccg
     5461 gatgcgaccc acgcaggttt ctgagcacac ggtgggttgg cctgactcaa ttcgcgggta
     5521 acagaagata catttttctg acttgccgct tttccagttg aagtagattt ttttgtacgg
     5581 acaaccgcta atgcacaaac gccagccacg gcatttatcc tgatcgatca gcacaatgcc
     5641 gtcttcttcg cgtttgtaga tagcgccgct tgggcaggtc gccacgcaac tgggattgag
     5701 gcagtgttcg cacaagcgcg gcaggtacat catgaaggtg ttttcaaact gcccgtacat
     5761 ctccttttgc atggcctcga agttgcggtc acgggcgcgt ttttcgaatt cgccgcccaa
     5821 caattcttcc cagtttggcc cccagatcac tttgtccatc cgtttgccgt caatcagtga
     5881 acgcgggcga gcagtaggga tatgtttgcc ttccggcgcg ctatgcaaat gatcgtagtc
     5941 gaaggtgaaa ggttcgtagt aatcgtcaat ctgcggcact accgggttgg cgaaaatttt
     6001 ggtaatcacg cccattttgc tgcccagacg cgggcgtatc ttgccattca catcacgcac
     6061 ccagccgcct tgccactcat cctgatcttc ccagtttttc gggtaaccaa tgcccggttt
     6121 ggtttcgacg ttgttaaacc atgcgtactc catgccttcg cgtccggtcc agacgttttt
     6181 acaggtcacc gaacaggtat ggcagccgat acatttatcg aggttaagca ccatgccgac
     6241 ttgcgaacgt attttcattt tttcgcctcc tgaacctgat cgcgaccttc atcatccagc
     6301 cagttaacgt tcttcatttt gcggatcata atgaattcgt cgcggttcga gccgacggtg
     6361 ccgtagtagt taaagcccca ggccagctgc gcgtaaccgc caatcatatg cgttggtttc
     6421 gggcaaacgc gggtaaccga gttatgaatg ccgccgcgca tgccagttac ttccgaacca
     6481 ggaatattca taatgcgttc ctgggcgtga tacatcatgg tcatgcccgg cggtacacgt
     6541 tggctgacca ccgcgcgggc agtcagcgcg ccgttggcgt taaatacttc cacccagtcg
     6601 ttatcgacaa tggtcagttc tcgggcatcg gtttcgctga tccagacaat cggtccaccg
     6661 cgagagagcg tcagcattag caggttttca ctgtaggttg agtgaatgcc ccatttctgg
     6721 tgcggcgtca ggaagttaag tgctttttcc gggaagccgt ttggcggtat ctggcgcatt
     6781 tcactgacgc tacgggtgtc gataggcggg cgataagcca ccagactttc accaaaagca
     6841 cgcatccacg gatgatcctg atagagttgc tggcgtcccg acagcgtgcg ccacggaatt
     6901 aactcatgaa cgttggtgta tcccgcgtta taggaaacgt gatcgctttc cagaccagac
     6961 catgtggggc tggagataat tttacgcggc tgcgcctgaa tatcgcgaaa gcgaatcttc
     7021 tcgtcctctt tgtgcagcgc cagatgggta tgttcgcgtc cggtgatctc gcccagcgct
     7081 tgccacgctt tgactgcaac atggccgttg gtttccggtg ccagcgccag aatcacttct
     7141 gatgcgtcaa tggcggtatc aatcagcgga cgcccctgtg ccgggccatc acgcttggtg
     7201 taattgagtt taccgaggaa atcgatttca tcctgggtat tccacgaaat ccctttaccg
     7261 ccgttgccaa gtttgtccat caatggcccg agtgaggtaa agcgttcata ggtagcaggg
     7321 tagtcgcgct ccaccgccac aatattcggc gcggttttgc ctggaatcag atcgcattca
     7381 cctttgcgcc agtcgagcac ttcacacggc tgtgaaagct ctgccgggga gtcatgcagc
     7441 agtggttgta ataccacgtc ggtttctttg ccaagatggc ccacgcacac ttgcgaaaag
     7501 gctttggcaa tacctttgta gatttcccag tcgctgcgtg attcccacgc cggatcgacc
     7561 gccgcagaaa gcggatgaat aaatggatgc atatccgagg tattcatatc gtctttttcg
     7621 taccaggtgg cggtgggcag aacgatatcg gagaacaggc aggtactgga catgcggaag
     7681 tcgagcgtca ccagcaggtc gagcttgcct tcaatcgccg cagtttgcca ctcgacttct
     7741 tctggtttga tcccgtcgct ggcaccgagt tcctgaccct gaatcccact ttcggttccc
     7801 agcagatact tctgcatata ctcgtggcct ttgccggagg agccaagcag gttagaacgc
     7861 cagacaaaca aattacgcgg atgattgctg ccgctatctg gttgttcgca ggccatgcgt
     7921 aaatcgcccg atttcagcgc ctgggcggta aattctgtgg gggataatcc ggccttgtcg
     7981 gcttcagctt taatcccgag cgggttacgc cccaactgcg gcgcagaagg tagccagccc
     8041 atacgttcgg cgcgaacgtt gaaatcaatc aggtgaccgc tgtatttact ggcatcggcg
     8101 agcggtgaga gcaattcctg cgcggtgact ttttcatagc gccactggct ggagtgattg
     8161 tagaaaaacg aggtgctgtt catctggcgc ggtggtcggt tccagtcgag cgcaaaggcc
     8221 agaggcagcc agccggtttg tgggcgcagt ttttcctgac cgacatagtg tgcccagccg
     8281 ccaccgcttt gcccgacaca accgcagaag atcagcatat tgatcatccc gcggtagttc
     8341 atgtccatgt gataccagtg gttaacacct gcgccgagaa taatcatcga gcgcccatgc
     8401 gttttatggg cggtatcggc aaattcacgg gcgatggttt caatatactg gcgcggaacg
     8461 ccggtaattt gctcgcccca ggcaggggta taaggtttga tttcagcgta atctttcgcg
     8521 ctgttttcat cttccagccc gcgatcgagg ccgtaattcg ccagtaccaa atcataaacg
     8581 ctgaccaccg gacaggtgct accatcggct aatgtcaggt ttttaacggg caattggcgc
     8641 accaggacag gattgtgttt tacgctgcga aaatgtggat tttcaatgcc gccaaagtag
     8701 gggaaggcca cgcctgcaac ggcgtcatgt tgaccgagca gggttaacga caattcggtt
     8761 tccgtaccgg cggcaatgga ttccaggttc catttgcctt tttctcccca gcgaaaacca
     8821 atcgaaccgt tcggcactac cagttcacct gcggtattaa ctgccacggt tttccactga
     8881 ggattattgc tttcgcccag tccatccacc aggtcagatg cgcggaccat gcgcccggga
     8941 acgtagctgc tgtcgtcgcg aggttccagc attaccagca tcggcatgtc gctgtagcgg
     9001 cggcagtagt tgataaagta gtcgctggga ttatcgagat gaaactcttt taaaatcaca
     9061 tggcccattg ccatcgccag ggcgctatca gtgccttgtt tcggtgccag ccactggtcg
     9121 cacaatttgg ccacttcaga gtagtcaggg gtaatggcga tggttttagt gcctttgtag
     9181 cgtacttcgg taaagaaatg ggcgtccggc gtacgtgtct gcggtacgtt agccccccag
     9241 gcgatgatgt aactggagtt ataccagtcg gcagattccg gcacgtcggt ttgttcgccc
     9301 caggtcatcg gcgaggcggg cggcaggtcg caataccagt cgtagaagct taaacaggtg
     9361 ccacctaaca gcgacagata acgtgttccg gcggcgtaag aaaccatcga catcgccggg
     9421 atcggcgaga aaccggcaac gcgatccggg ccataggttt tgatggtcca gacattagca
     9481 gcggcaatca gctgatttag ctcctgccag ttggagcgga taaagccacc gcgtccacgc
     9541 acttgtttgt agctcaggca cttttgcgga tcgttcataa tcgatgccca cgccagaacc
     9601 ggatcgctgt gttgcttgag ggcttcgcgc cacagttcaa tcagtcgttt acgaatgagc
     9661 gggtatttca ggcggttagc gctgtagaga taccaggagt aacttgcgcc acgcgggcag
     9721 ccgcgaggtt catgatttgg caggtcaggg cgagtgcgcg ggtagtcggt ctgttggatt
     9781 tcccaggtca ccagaccatt tttaacgtag attttccagc tacaggagcc tgtacagtta
     9841 acaccgtggg tggaacgcac gattttgtcg aactgccaac gctggcgata gctgtcctcc
     9901 cagtcgcggt tgctatgcat cacctgtccg tgaccatcgg caaaggtttc gcccttttgt
     9961 ttgaagtagc gaaagcgatc caaaagttta ctcatgacat aactcctgct ccaggaatga
    10021 attgggatct gatgccttgt cggatgagac acggttgtag cctgataaga cgtgtcaggc
    10081 atcgatctct tatttttggc tgaacttccg ccgaccataa accagccagg tcagtagcac
    10141 acaaacgatg tagaagatta aaaacacttt catcgcgccc accggagagc cggtcatatt
    10201 gagcgacatg ccaaacgcct gcggaataaa aaagccgccc actgcgccaa tggctgagat
    10261 aaagcccaga gcggctgccg tttcggtgac ggcttctctt tgagcttgct catcactacc
    10321 gcctttcatc ttcacccgat aaattgttat ctggcgaaag atgacggcga tcatctggaa
    10381 agtagaacca cttcccagac ccgcggtcag aaacagcccc ataaatacgg cgtaaaatgc
    10441 gatgaaatta ccggagcctg tgcccggtaa ggtaaggaac agcagggcgc tgaaaatcgc
    10501 cataaaaatg aagttgatca acgtcacccg cacgccgccg aatttatcgg aaatagcacc
    10561 accaaccgat cgcgcgatgg caccgataaa tgggccaaag aacgccaggc gcagaatatt
    10621 cacatccggg aactgggttt ttgccagcat ggcaaaaccc gcagaaaaac cgataaacga
    10681 accgaaggtg gcaaggtaaa gcaggctcag cagccagaga tgcaggcgtt gtaagacagg
    10741 gagctggtcg gcaattgagg cgcgtgaact ggcgatatca ttcatcccag accaggcggc
    10801 gatcgtggca atcgccagta atggcaccca aatccatgcg gcattcgcca gcgacatcac
    10861 cgaaccgtcg gcctgcggta cgccattgac gccgagaaag gcaaatacgg ggacaaaaat
    10921 gaccagcggt gcaaccagct gcatcacact tacacctaag tttcctaatc cgccattaat
    10981 cccaagagcg ctcccttgtt tggcttttgg aaagaagaaa ctgatattgc ccatgctcga
    11041 agcaaagttc gcacctgcaa aaccgcatag caaagcgata acgataaata tcccaaaagg
    11101 agtattcgga ttctgcacgg caattccgag ccagacgcaa ggaataatca ggattgcagt
    11161 actaaaaacc gtccatcggc gtccaccgaa tataggcacc ataaaggagt aggggacacg
    11221 caataatgcg ccagaaacgg agggtaatgc ggttaataaa aagagttgat cggtagtgaa
    11281 attaaaaccg attttattga gattaacagt aactgcgcta aatagcatcc agacacagaa
    11341 ggcaagaagt aggcaactga ctgatatcca gaggtttctt cgtgcaatat gctttccttt
    11401 attttcccag aaggccggat tttctggttt ccagtcgcgt aaaagataac gactattttt
    11461 ctcattttgc agtgccatat tgttcctcac atgcacacat tggtaatgaa aaaaagacaa
    11521 aacaggaggt aaggcgcaat agccagttat tagaattaag gatgaatcag gtgaagtgct
    11581 gattagaaga atagataaga a
//
LOCUS       DAZKHK010000144        11530 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKHK010000144 DAZKHK010000000
VERSION     DAZKHK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.145"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..875)
                     /gene="uxuR"
                     /locus_tag="ACHOS5_004231"
     CDS             complement(102..875)
                     /gene="uxuR"
                     /locus_tag="ACHOS5_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP9778955.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNADSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1090..2550)
                     /gene="uxuB"
                     /locus_tag="ACHOS5_004232"
     CDS             complement(1090..2550)
                     /gene="uxuB"
                     /locus_tag="ACHOS5_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP9778956.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2631..3815)
                     /gene="uxuA"
                     /locus_tag="ACHOS5_004233"
     CDS             complement(2631..3815)
                     /gene="uxuA"
                     /locus_tag="ACHOS5_004233"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9778957.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4264..4371
                     /locus_tag="ACHOS5_004234"
                     /pseudo
     CDS             4264..4371
                     /locus_tag="ACHOS5_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4544..5446)
                     /gene="fimH"
                     /locus_tag="ACHOS5_004235"
     CDS             complement(4544..5446)
                     /gene="fimH"
                     /locus_tag="ACHOS5_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9778958.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSSFSGTVKYNGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(5466..5909)
                     /locus_tag="ACHOS5_004236"
     CDS             complement(5466..5909)
                     /locus_tag="ACHOS5_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778959.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLVSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(5982..6512)
                     /gene="fimF"
                     /locus_tag="ACHOS5_004237"
     CDS             complement(5982..6512)
                     /gene="fimF"
                     /locus_tag="ACHOS5_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP9778960.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAVKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNEIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6522..9158)
                     /gene="fimD"
                     /locus_tag="ACHOS5_004238"
     CDS             complement(6522..9158)
                     /gene="fimD"
                     /locus_tag="ACHOS5_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP9778961.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTSWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(9224..9949)
                     /gene="fimC"
                     /locus_tag="ACHOS5_004239"
     CDS             complement(9224..9949)
                     /gene="fimC"
                     /locus_tag="ACHOS5_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9778962.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESAVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(9986..10525)
                     /locus_tag="ACHOS5_004240"
     CDS             complement(9986..10525)
                     /locus_tag="ACHOS5_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9778963.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAEACR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSSPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(10590..11138)
                     /gene="fimA"
                     /locus_tag="ACHOS5_004241"
     CDS             complement(10590..11138)
                     /gene="fimA"
                     /locus_tag="ACHOS5_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9778964.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GNHPNVLALQSSAAGSATSVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         2796 a         3026 c         2757 g         2951 t
ORIGIN      
        1 taatgattaa tttacgcata atatattctg cagaacataa ttaactattc agggaacata
       61 catagtgcaa agcggttaaa ctttgcacca gaaatgaggg atcaggcgtc gactttatcc
      121 agcggccatg aatcaaacag atagccatcg aaatagatgt catcaacatt agagaactcc
      181 agcaagcgct gcttcacatt ctccagatgc tgccacattg ccagcttcgc cgctcgggca
      241 tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac
      301 aggctgtcat ccaggtggct gtgcaactgg atccacattg gattgttttc ccgccactgc
      361 caggactgac ggaataactc caccagcatg ctgttatgcg ttgcttcggc aatagcgaga
      421 tggaactgca tgtcaccgct ttcgctgctg cccggcgcgc tggaagccag ttcacgctct
      481 tccagttgca atgcctggcg cattttgacg atatcttcgc gggtagcctg cagggcggca
      541 aactcggcga tgttactttc cagtaactgc cgcgcctgta acagctcaaa aggaccggca
      601 tcgttgcaga cgttggcatc cggactgtct gcgttgtggc tgcctgagct atcaagaaca
      661 tagatacctg caccccgacg cacttccacc agccctttga tctccagcat gatcagcgct
      721 tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggtggcagc
      781 cgttcgccag gattgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc
      841 tggtaaggtc tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt
      901 aatcaaattt tttccggatg cggcgtgaac gccttatccg acctacaacg ggcaatttta
      961 cggcacccgt taagctcaaa ctataccatt agcactggat gttggcacgt acaggtgggc
     1021 aaaaaaacga aaactggtca accaaatatt agtttgattg accagtaagt ctgtagggta
     1081 atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgctcacaca gctgctgata
     1141 tgccgccgtc accgcgccaa caaagtcggc gttctgcggc agatcatcgg caaagatacc
     1201 gctcaggccc agcagcgctt tcacacggtc tgcgccctga tactgcgcat tgatcttctg
     1261 gaactccgct agcatcgggt caaccacgtc aatggcatta ccctgctcat ccacgccctg
     1321 ggtgtagcgc atccagcccg ccacgcccag cgccaggtga cgccagctgc cgccgttttg
     1381 caggtgcaga cgtaccggat ccagcagacg ctgcggtaac ttctggctgc cgtccatcgc
     1441 aatctgccag gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc
     1501 ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat
     1561 cagggcaaag gccgctttgc gataagccgg gttagtcatg gtgtcggcaa tagtttcata
     1621 gccgccgagg taaccgaggt acgccaggaa agagtggcta ccgttcagca tacgcagctt
     1681 catcatttcg aacggtacaa catccgcaac aaactgcgcg ccgactttat cccagtccgg
     1741 acgaccatta acgaagttat cttcaattac ccactgacgg aacggttcgc aggcaatggc
     1801 gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc
     1861 cggaacgatg cggtcaacca tggtgcacgg gaaggtgacg ttttcttcaa tccatgccgc
     1921 cagttgcggg tcacgcgcct gagccagccc aagaacggca accttcgcca catgaccgtt
     1981 ttcacgcacg ttatcgcagg acatcaccgt aaacgctttc agcccttttt cacgacgcag
     2041 ccgcagggct tctacgatgt aaccaatcgc agacttcggc gcagtcgggt tttccagatc
     2101 gtgcttgatc agcgggttat tgagatccag ctgaccgctt gccgcatcag cgcagtagcc
     2161 tttttccgtg accgtcagag agacaatcgc cgtctgcgga cgcgccatcg cgttgagaat
     2221 accttcgcaa ccgtcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag
     2281 ctcggtgctt tccgcgcctt tttccgccac ggtgtacagc agttgctgtt ttttcaggtt
     2341 ttcgatcagc acgcggtcgt tgcctggcat caggttaact tcgcagatac cccagtcgct
     2401 gtcggtactt tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc
     2461 gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac
     2521 cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcagagagt
     2581 gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtggata ttaacggcta
     2641 aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga
     2701 ccaattgcgg agtaacctgg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg
     2761 tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct
     2821 tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg
     2881 tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc
     2941 ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg
     3001 gtgcacatgg tgaaaccgtt cgccatgctg tttacggtat caaccatcca ctgcatatct
     3061 tcaatggtgg aaacaatgcg cggcaggccg aggatcgggc gcggcggatc gtccgggtga
     3121 accgccatac gcacgccaac ttcttcagca actggaataa tcgctttcag gaagacagca
     3181 aagttttcgc gcagtttggc tttgtcgata tctttgtaca gctccaggtg cttacggaac
     3241 tggtcgaggg tatacccttc ttccgcacct ggcagaccgg caatgatgtt acgggtcaga
     3301 cgcgctttgt cttcgtcgct catagtggcg aagcgttcag cggcctgagc aatttcttct
     3361 tcggtgtaat ccgcttccgc gcctggacgc ttcaggatat gcatttcgaa tgcagcgaat
     3421 tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta
     3481 cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc gcactgcgcc
     3541 aggttgcgca gggtctgctg atagttagca atccactgct catagttgcc agtgtgggtt
     3601 ttgatatctt cgtgaattgg tacgctttct acgacagacc ataccaggcc tgcgtcttca
     3661 acgatcgcct tgcgtttgag gatctcttca acggaccata cttcgccgtt cgggatatgg
     3721 tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga
     3781 tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg
     3841 ttcaacagcg aagagacgcg gaaccccgga gttgtttagg tatccggatg aacaacccgg
     3901 ccacagccgt agcgtcgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca
     3961 tgtcaacccg aattctaaaa ttggttaacc acatcacaag aatttcactc tcctggttgt
     4021 tgctgaaaat tgcagcaaag ggtaatcgat ggttattgat cagaggatgt gacattcatc
     4081 gcaacaatgg ttgaccaatt tacataacat gtcgcccaaa ataacaacgg ttcaaccagg
     4141 taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt ggtattcggc attggcctga
     4201 tgctggtact gacaatgacc gcaggttcag acacattccc tacattagca atgcctctta
     4261 gattactttg acctgtcagt aaaagacacg ttgaaaacct ggggtctgct ggagctggtc
     4321 aactccgtgg ttgggctgat tattgtgctg attattagca tggtagcgta aaagagcacc
     4381 tgagcctgcc attggcaggc tctgttcgga ttgtcggtaa agtgcgcttt gtttatgcca
     4441 gatgcgacgc tgacgcgtct tatctggcct acaaagggct aacgtgcagg ttttagcttc
     4501 aggtaatatt gcgtacctgc attagcaatg ccctgtgatt tctttattga taaacaaaag
     4561 tcacgccaat aatcgattgc acattccctg cagtcacctg ccctccggta cgtgcgtaat
     4621 ttgccgttaa tcccagactt accgccgaag tccctactgc tcctaacgat accgtgttat
     4681 tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc gacgccctgc gcgggtgaaa
     4741 acgacgcggt attggtgaaa atcgagttgc ccgcatctgc ggttgtgccg gagaggtaat
     4801 accccaggtt ttggcttttc gcacaataaa cggtaagagg aatcggcact gaaccagggt
     4861 agtccggcaa agtaacggtg acatcacgag cagaaacatc acagccgcca gtgggcacca
     4921 ccacatcatt attggcgtaa atattccaca caaactggaa atcatcgctg ttatagttgt
     4981 tggtctgtcg caaaataagc acggcaatta atgagccagc tttaatcgcc actccccccg
     5041 cactgctcac aggcgtcaaa taaagcgcca ccggccacgg cttatccgtt ctcgaattat
     5101 aaacaacccg cggcgtttcg ctggtagtag ggaaaggata gctactgcca ttatatttta
     5161 cggtcccgga aaaactagat aacacgccgc cataagccga acctcgttgc agtgtgacat
     5221 agtctgtaat ggtttccggg taatcgttat ggcaaaagat ttgcgtcgaa agatccacga
     5281 ccaggttttg ccccacattc acggcaggcg caaggtttac ataaacattg gcgctgccac
     5341 cgccaatagg gatagcggta ccattggcgg ttttacaggc gaatgaccag gcatttaccg
     5401 accagcccat cagcagtaca gcaaacaggg taataactcg tttcattaca atcatctctt
     5461 cgggttcagc tgtaggtata ggtgatgcta atcactgcct gaatggttcc ctgagtggct
     5521 ccgccattta ccgtcaatgc tctgacctgt aacgggaagt gcgctgattg tgaggaatca
     5581 tccacctgaa ctgttttggt tgcgccagta ttcaatgtgt tgccactgtc atcctgtagc
     5641 tctaactgga tgttttgcgc ggtcccctgg tttttataat atccggtact atcggctgcc
     5701 ccgctgaagc tggcagtgac cctcgacgtt cccaccggac aattagtcaa ctcaagcgca
     5761 acatcatgcc aggccgatgc cgccccggca gacacgagac tgaaagaata aagatcgccg
     5821 agatctaccg tggcattggt ggtggaaacc gtacacggtt tggcgacgac cttaccgttc
     5881 accgtgatgg tgacatcggc tgcctgtatc gtcgcacttg cgaacgccaa cattgccgcc
     5941 aatacatacc cacgtttgcg ccatttcatg agcatctcca gttactgata ttcaagagtg
     6001 aaggtagccg tggcattgat atgccccgca gtgacaggca cctgtgtcgc cattagccgg
     6061 gcgtaaaaat tcaacgtatt tggtttaccc ggcgtcaggg tcgtccacga aatcgcggac
     6121 gatggggcat taaggggtat ctcattttgc tgctcattca gaagctgtat tcccagtccc
     6181 gcagccgctg acaccgtatt ttcaagtgca agcaggttgg cattgtggct atctgcaacg
     6241 ccagtaaacc caacctttac ggcagaaaca gcgttaccac agggtgacag caaaatacga
     6301 aatggaacga caggagtcgt cgcgccaatg ttgttaaatt gcttcaccgc gttttccatc
     6361 agatcaacag taaaattggt tgattcagcg gccacactac agccattatc tctgacatag
     6421 ccgcggatag taatcgtgct atccgcagcc aaagcgtgac ttaccgccag ccacaaaaaa
     6481 gcgcaaagaa gataaaaagg tttgtttctc atcacgcccc cttaacgaca ttcagctgat
     6541 agctgggtta ataactgctg ctgactctct ggtggcagtt gataattggc gacacagtga
     6601 gcattttcct cttctcccca tttcacctgc acttttcccg ctaaaggcat tccgctgagg
     6661 taaacctgac cattatccgc aacaatgccg ctactctggc tactctctga tgtcaccatc
     6721 gccccaaacg gcagcggctt attattgtgg gttagcgtca tgagcagttt tatcccaacg
     6781 cgcgctttaa actctgctcg cacgatcgcc ccacgggtgg gaacaacgtt agcgaccgca
     6841 ttatctaaat cgacgttatc agccagggta ttggtatcca gcgccactct gttttcccga
     6901 tattcagtgg cataaggcag cacggcataa ccccgccagt cggtacgcac tcctgtctgg
     6961 ttttcgactt ttgcatcttt tgcgccaggc gctttaacaa gcaccaccgt atcgtttaac
     7021 ggctgcccca gcgttacgcc attggcatga gccagtaccc caccgctgac tccgtaatag
     7081 agctgcttaa tatcatcgct atggctgtaa ccgatattgg cattgccgta accaccgcga
     7141 taattcagcg tggcgtagcc tgtacttccg ctatttccat cgcctccccc ggcataaccg
     7201 gtttgcacgc tatagctgag gttgttgtct tccagcaacg taccgtatac accggccaga
     7261 ttggtcatcc gaccgttgag atcgtgtgac atgctgtaac tggcactggc atgtcgccac
     7321 tgagatttac tgtcagaacg cagccagtgg ctgaaaggaa tattgacgtt aagcgctaac
     7381 atctgatccc gtcctttttg ccaggcgttt ttcgtcaggc tatagctgag cgtccagtta
     7441 atatcttcga acgcagtatt taatccagcc tggaattgct catcgacatt actcgttccc
     7501 caataagttt gatggctacc actcaaatac agtgttgatg tgcgcccgag ttgctgagta
     7561 acggtgagct gtaatttccc gcgtttgtta taagcgagat tgtaatagtc ggtgaatttt
     7621 ggcttaacct gaataacacc atcctgtgtt tcgatgttgt agccattcat tcgactgtat
     7681 gttgtatcag cgaaattaaa atatccgctg gtcgaataac ggtaacccac taactgaata
     7741 ttcgtgcctg actcattgag cgatttgtta tagagaaaac gcaccgattg tccgtcatgc
     7801 tgactgtcat cgggaagtgt ggaattagcc tgcgtcatat ccacagacag agcgcccagt
     7861 gcccccatat ttttcccgat accgaaatta aacgcacgat aacgatccgc cagttgcgtt
     7921 ccaccatata ttgtccagcc agccggaagg ccgtggagta atgtactctg gaaaaagcgg
     7981 ggtttttcct gttgcgcatt tccactacgg tattctcctg ccgtaatgga ataacgtgta
     8041 tgcccttcac gctgcaaaag cgggactgac gaatagggta cggtaaaaat ctgcgtactg
     8101 ccgtcagcct ctttaatcgt tacctgcaag tcaccactat tacctgcggc atagatatcg
     8161 ttgatggtaa aaggccccgg tggcaccgta ctattataaa tgtcataccc attttgttta
     8221 atagtgacct gtgcagtacc acgagcaata ccgtggatca ccggggcaaa tcctctttgg
     8281 ctatcgggta acatattgtc atctgaggcc aattgtgcgc cgcgaaagtt aataccatcg
     8341 aaaatatcgc cctgagtata accatcaccc agcgtcagcc gggaacgtaa cggaattatg
     8401 tctcgctcaa gccaggtatt gatatgctgc catttatttt tgctacctga tgaactgtcg
     8461 ctactgttat agctccagct ggtattgtcg cgtaaacgcc acgcaccaat atttaaacca
     8521 ctctgtaggt ttaaatatgc ataatggctg ttacccccaa tccgattctg tacactattt
     8581 ccgctgaaat tataattgag caatccggca ttaataccgg gatcccataa ctcaggagga
     8641 atataaccac gcgcgcgatt actcataaat gcctgaggga tcgtcaggtt gagtcgctgc
     8701 tgacccacat ccagttgcgc agtagcgtca tggatcattg aggttaatgg cacgcaggca
     8761 tcatccgcca gcagattcat accggagaca gaagccgtat tcagccccat actggcaagt
     8821 tgcgcgcgtg tcaggcaggg aacaatccct tgttcgctgt cgcccgtatt aaatgtgaca
     8881 tcacgcgttg ccatataacc attattcaaa tagatatcaa cacgatacgt ccctggcggt
     8941 aattcttgcc cattttcaaa acgcgataaa tcagccacag cctggggatc atccgctaaa
     9001 aaacgcgggt taaaatagag ttcggcagat gacaaaggtg cctgtgcggc aaaagcacag
     9061 gcaacaaaga gtcggacaaa aaaaccagcc aaacgatgct tacgaatatg caagcattgt
     9121 gtgtttcgct ggtaaagtct taaattcaga tatgacatta ttcctccggt ttaaatcacc
     9181 cggcagtcaa ttctttttat tcaggcgaaa aaatccccct gcgttattcc attacgcccg
     9241 tcattttggg ggtaagtgcg ccataatcat ttattgttcg gtaagtaata ttgcttcctg
     9301 catcagaagg caatttaacc gcgctttcgc ccattggagg caccaatgca ttttcaagaa
     9361 cccgagttcc ggcattcaac tctgttaccg tcaggtaata gggtgtcggg ttgatcagcg
     9421 tcagagaatt cgcgctacga cgaaatctta atttttctgc ggcctgatcg ggtggcaacg
     9481 ctaatttagc cggacgatag tacagtttaa tgcggctgat aattgcgagc tgtagcgtat
     9541 tctcagtcaa ttttgattta tccattgacg gaatcgcttt aacattcatc cagaataaac
     9601 tttcccgatc ctgtggcaat tggttatttg ttgcatcaag aatacgcaag gtattctctt
     9661 tttttccctt catcgcaaac agaggtggcg tcacgataaa acggccatcc tttacaccat
     9721 cggcattttc tacccatgat tgaattaaat aggtgctatt ttcatcatta tttgtcacgg
     9781 caagttgcac ttgtttttgc cctgccggat aaattacgcg agtcgcacct aaggccactc
     9841 ccgcttcagc gcgtccggca accatcattg ccatgaacat caggatacct gccagcaagc
     9901 agaatgttat ttcctgcgat ttccttacat tgacgttttt attactcact gtcctgttcc
     9961 tgttatcaca ttatctgctg aacaattact gataggttaa agagaaccag gcctgggcat
    10021 tggcgatgcc gccagtaacc cgacgcccgg tagcacgata tttggcgatg aaatgtagcg
    10081 acgtagagcc tgagtaaagc cgtttccagt ttgctggagg acggttaatc ggtacgaggt
    10141 ttccttcatc atcaaacaac gctacgccaa tattggtggc tatccctggc ccctctccca
    10201 cggaaagcac atccggattt ttaccatccg cgacaccgtg aaacgccaca cccacacgtt
    10261 cactcaccac cgtgctacat tcccgtaaat gaataacaaa aggcaccggt gagctatctt
    10321 cccctaccgc atgaaaccgg ttactgctga tttgccccat attgaccgtc atttgtttat
    10381 caccggcttc aatccggcaa gcttccgcaa taatgacgcc ctgaaattgc atatttccgc
    10441 cgggcagcgt ggtattccat ttatttccgg ccagagcaaa cattggcagc aacgacgcta
    10501 acagaaataa tctcttcctt ttcatcgtta ttttgatcgc acaagggtgg gcatccctgc
    10561 ccgtaatgac gtccctgaac ctgggtaggt tattgatact gaaccttgaa ggtcgcatcc
    10621 gcattagctg cacccggggt tgcctcgccg attgcataat aacgcgcctg gaacggaatg
    10681 gtgttggtac cgttattcag ggttgtttgt gcactaaatg tcgcaccgtc cagcgtcaga
    10741 gcattgcctg ttctgtccag aatttgcaca ccaacgcttg ttgcgctacc tgcagcagaa
    10801 ctctgcagag ccaatacgtt aggatgattt ccatcaattg cggtaccaag gaaggcaata
    10861 gctgctttgt ctgcaacagt agtgtcacaa tcgttcagtt gaatgttaaa accgacagca
    10921 gaactggttg ctccttcttg tgccagcgat gcggtacgaa cctgccctaa ctgaacggtt
    10981 tgatcaacag agcctgcatc aactgcgcaa gcggcgttaa caacttcccc tttaaagtga
    11041 acggtcccac cattaaccgt cgtggcagcg gccagagccg ctgtggaact gagggacaga
    11101 gccgacagaa caacgattgc cagagtttta attttcatgc tgctttcctt taaaaaaact
    11161 atttctaaat cgacatgggc agtcgttctg tacactttgt tttgtcaacg agtttgactg
    11221 ccaacactgc acagttttcc cccaaaagat gaaacatttg gggccatttt gactcataga
    11281 ggaaagcatc gcgcacaaac tttttcagtt tatttgttgg cttaatgctc tatagttatc
    11341 tttatttgca gttttttata ttgcatgagg tggtttttgg agagaagaat gaggaagttg
    11401 cgtcgagcta cagaaacgtt agctttacat atagcggagg tgatgtgaaa ttaatttaca
    11461 agagaaataa tttacatatc aaacagttag atgctttttg tcgtttttta atatttttat
    11521 gcttgagaaa
//
LOCUS       DAZKHK010000145        11502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKHK010000145 DAZKHK010000000
VERSION     DAZKHK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.146"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..982)
                     /gene="pyrD"
                     /locus_tag="ACHOS5_004242"
     CDS             complement(<1..982)
                     /gene="pyrD"
                     /locus_tag="ACHOS5_004242"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP9778965.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPP"
     gene            1219..1794
                     /gene="ssuE"
                     /locus_tag="ACHOS5_004243"
     CDS             1219..1794
                     /gene="ssuE"
                     /locus_tag="ACHOS5_004243"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9778966.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQVQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            1787..2746
                     /gene="ssuA"
                     /locus_tag="ACHOS5_004244"
     CDS             1787..2746
                     /gene="ssuA"
                     /locus_tag="ACHOS5_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP9778967.1"
                     /translation="MRKIIKLVLAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            2743..3888
                     /gene="ssuD"
                     /locus_tag="ACHOS5_004245"
     CDS             2743..3888
                     /gene="ssuD"
                     /locus_tag="ACHOS5_004245"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP9778968.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            3900..4691
                     /gene="ssuC"
                     /locus_tag="ACHOS5_004246"
     CDS             3900..4691
                     /gene="ssuC"
                     /locus_tag="ACHOS5_004246"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP9778969.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            4688..5455
                     /gene="ssuB"
                     /locus_tag="ACHOS5_004247"
     CDS             4688..5455
                     /gene="ssuB"
                     /locus_tag="ACHOS5_004247"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9778970.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWLKHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(5650..8262)
                     /gene="pepN"
                     /locus_tag="ACHOS5_004248"
     CDS             complement(5650..8262)
                     /gene="pepN"
                     /locus_tag="ACHOS5_004248"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP9778971.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            8528..9730
                     /gene="pncB"
                     /locus_tag="ACHOS5_004249"
     CDS             8528..9730
                     /gene="pncB"
                     /locus_tag="ACHOS5_004249"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9778972.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLHQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9898..11298
                     /gene="asnS"
                     /locus_tag="ACHOS5_004250"
     CDS             9898..11298
                     /gene="asnS"
                     /locus_tag="ACHOS5_004250"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9778973.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2641 a         2968 c         3034 g         2859 t
ORIGIN      
        1 gcggcgggcc tttaaaaata aaaccagaat aaatttgcac cagtgaggca cccgcagcaa
       61 tcttttcacg cgcagcgata accgagtcaa tgccgccaac accaataatc ggcaagcgtc
      121 cgtttaattc ctgtgacaag cggcgaataa tttcggtgct ttttaactga agcggacgac
      181 cacttaagcc accggtttga tcacaatttt tcattccctg aaccagagaa cgatcgagtg
      241 tggtattggt tgcaataacg ccatcaatat tatggcgaac taaactatcg gcaacctgga
      301 tcaattcttc ttcagaaaga tccggcgcga tcttcactgc gatcggcaca tatttatggt
      361 gcatcacttg caaatcattt tgtttatttt taatcgcagt taagagatca tccagcgctt
      421 caccatattg cagtgtgcgt aatcctgggg tatttggcga tgaaatattg atggcgatat
      481 atcccgcata ggcatagatt ttttccatac aaatcagata gtcatcttta ccctgctcca
      541 ccggcgtatc tttatttttg ccgatgttaa tacccagaac gccgtcataa tgggcttttt
      601 ttacgttctc tacgaggtta tcaacgccaa gattattaaa gcccatacgg ttgatcaaac
      661 cttcggcatc taccagacga aagagacgcg gcttgtcatt acctggctgg ggacgtggcg
      721 tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc taacgcgtca atgcactccc
      781 cgtctttatc aagaccggcg gccagaccaa gcggattttt aaacgtcagg cccatgcagt
      841 taacaggttt cgcaggcact ttctgccgca ccagtgcttc aaacggcgtt ccggtaatac
      901 ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg atcgagctgg aaaagggctt
      961 tacgaacgaa ggggtagtac atgaactctc ctggattccc ggtgtgcaaa ccgggggcgt
     1021 attatgtgcg agttcggacc aaaagggaat tgacctgcgg caataaatag caaacgtttt
     1081 cttattgtgc tttttttatg ctttttttgg cttttcttcc tcagataaat catttcgact
     1141 cgcggacgcc ctctgtcaga ctgaccgaat tgttatcaat gttaacaaaa caagaacaat
     1201 tggttataag gagagagtat gcgtgtcatc accctggcgg gtagtcctcg ctttccttct
     1261 cgctccagct ccttgctgga atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt
     1321 tatcactgga atttgcaaaa cttcgtcccg gaagatctgc tttatgctcg tttcgatagt
     1381 ccggcactca agaccttcac cgaacaggtg caacaggccg atgggctgat tgtcgccacg
     1441 cctgtgtata aagccgccta ttccggtgca ctgaaaaccc tgctcgacct gctgccagaa
     1501 cgtgcgttgc aaggcaaagt ggtgctgccg ctggcgacag gcggcaccgt ggcccatctg
     1561 ctggcggtcg attatgccct taagccagtt ttaagtacgc tgaaagccca ggagatcctg
     1621 cacggcgtgt ttgccgatga ctcacaagta attgattacc aacacaaacc ccagttcacg
     1681 ccaaatctgc aaactcgtct tgataccgcg ctagaaactt tctggcaggc gttgcaccgc
     1741 cgcgatgttc aggttcctga cctactgtct ctgcgaggta atgcccatgc gtaagatcat
     1801 taaactggtg ctggcgggtt tacttagcgt ctctacactt gctgttgccg cagaatcctc
     1861 gcctgaagcg ttacgtatag gctatcaaaa aggcagtatt ggtatggtgc tggcaaaaag
     1921 ccaccagtta ctggaaaaac gctatccgca aacaaaaatc tcctgggtcg agttccccgc
     1981 tggcccacag atgctggagg cgttaaacgt tggcagtatc gatctcggca gtaccgggga
     2041 tattccgcca atctttgccc aggctgccgg ggctgatttg gtatacgtgg gcgtcgagcc
     2101 accgaagccc aaagccgaag tgattctggt ggcagaaaac agcccgatca aaaccgtagc
     2161 cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc agttcgcaca atcttttact
     2221 gcgagccctt cgccaggccg ggcttaaatt cacggatatt cagcccactt acctgacgcc
     2281 tgccgatgcc cgcgccgcgt tccagcaagg taacgttgac gcctgggcta tctgggatcc
     2341 ctactactcc gctgcattat tacagggcgg cgtgcgggtg ctgaaagacg gcaccgatct
     2401 caatcaaact ggatcgtttt atctggcagc tcgtccgtat gcagaaaaaa acggcgcttt
     2461 tattcagggt gtgctgacaa cctttagtga ggccgatgcg ttaacccgca gccagcgcga
     2521 acaaagcatc gctttactgg caaaaacgat gggcttaccg gcaccggtta ttgcctcgta
     2581 tctggatcat cgtcctccca ccaccatcaa accgttgagc gctgaagttg ccgccttaca
     2641 gcagcaaacg gcagatctgt tttatgaaaa ccgtctggtg ccgaaaaaag tcgatattcg
     2701 ccagcgcatc tggcagccca ctcaactgga aggaaaacaa ttatgagtct gaatatgttc
     2761 tggtttttac cgacccacgg tgacgggcat tatctgggaa cggaagaagg ttcacgtccg
     2821 gttgatcacg gttatctgca acaaattgcg caagcggcgg atcgtctggg ctataccggt
     2881 gtgctgatcc cgacggggcg atcgtgtgaa gatgcgtggc tggtggcggc gtcgatgatc
     2941 ccggtgacac aacggctgaa gtttcttgtc gccctgcgcc ccagcgtaac ctcacctacc
     3001 gttgccgccc gtcaggccgc cacgcttgac cgtctctcta acggacgtgc gttgtttaac
     3061 ctggtcacag gcagcgatcc acaagaactg gcaggcgacg gcgtgttcct tgatcatagc
     3121 gagcgttacg aagcctcggc agaatttacc caggtctggc ggcggttgtt gcttggcgag
     3181 actgtggatt tcaacggcaa acatattcat gtacgcggag ccaaactgct cttcccgccg
     3241 attcaacagc cttatcctcc gctttacttt ggcggatcgt cagatgtcgc ccaggagctg
     3301 gcggcagaac aagttgatct ctaccttacc tggggcgaac caccggaact ggtaaaagag
     3361 aaaatcgaac acgtgcgggc gaaagctgcc gcgcatggac gcaaaattcg tttcggtatt
     3421 cgtctgcatg tgattgttcg tgaaaccaac gacgaagcgt ggcaggccgc cgagcggtta
     3481 atctcgcatc ttgatgatga aactatcgcc aaagcacagg ccgcattcgc ccgcacggat
     3541 tccgtaggac aacagcgaat ggcggcgcta cataacggca agcgcgacaa tctggagatc
     3601 agccccaatt tatgggcggg tgttggctta gtgcgcggcg gtgccgggac ggcgctggtg
     3661 ggcgatggtc ctacggtcgc cgcgcgaatc aacgaatacg ctgcacttgg catcgacagt
     3721 tttgtgcttt cgggctaccc gcatctggaa gaagcgtatc gggtcggcga gttactgttc
     3781 ccacatctgg atgtcgccat cccggaaatt ccccagccac aaccgctgaa tccacaaggc
     3841 gaagcggtgg cgaatgattt tatcccccgt aacgtcgcgc aaagctaagg aacacgatga
     3901 tggcaacgcc agtgaagaag tggttattgc gcgttgctcc ctggttttta ccggtgggca
     3961 tcgtggcggt gtggcaactg gcctcctcgg ttggctggct atcgacgcgt attttgccct
     4021 caccggaagg agtggtgatg gcgttctgga cgctctctgc cagcggcgaa ctgtggcagc
     4081 atctggcgat cagctcctgg cgtgcgctga ttggtttttc aattggcgga tcgctgggat
     4141 taattctggg gctgattagc gggctgtcgc gctggggaga acggctgctg gatacctcca
     4201 ttcagatgtt gcgcaacgtg ccgcatctgg cgctgatccc gctggtgatt ttgtggttcg
     4261 gcattgatga atccgcaaaa atatttctgg tggcgctcgg tacgctattc cccatttata
     4321 tcaacacctg gcatggcatc cgtaatatcg accgtgggct ggtggagatg gcacgtagct
     4381 atggattatc cggcataccg ctgtttatcc atgtgatcct gcctggtgcc ctgccctcaa
     4441 ttatggtcgg tgtgcgtttt gcgttgggcc tgatgtggct gacgctgatc gttgccgaaa
     4501 ctatttctgc caattccggc attggttatc tggcgatgaa tgcacgggag tttctgcaaa
     4561 cggacgtggt ggtagtcgct attattcttt acgccctgct cggcaaactt gctgacgtca
     4621 gcgcgcagct gttagaacgt ctctggttgc gctggaaccc ggcttatcat ttgaaggagg
     4681 ccacggtatg aatacggctc gtctgaacca gggcacacca ttgttgctca atgcagtaag
     4741 caaacattac gcggaaaata tcgtcctgaa ccaactggat ttacatattc cggcaggtca
     4801 gtttgtggcg gtggtgggcc gcagcggcgg tggcaaaagt actctgctgc gcctgctggc
     4861 aggtctggaa acgccaaccg caggcgacgt gttagcgggc accacaccgc tggctgagat
     4921 tcaggatgat acgcgaatga tgtttcagga cgcgcgtctg ctgccgtgga aatcggtgat
     4981 tgataacgtt gggttaggcc ttaaaggcca gtggcgcgat gcggcgcgtc aggctctggc
     5041 tgcggtagga ctggagaatc gcgcggggga atggcctgcc gcactttctg gcgggcagaa
     5101 acaacgtgtg gcgctggcaa gggcgctgat tcatcgacca ggtttattgt tgcttgatga
     5161 accactcggt gcgctggacg ctttaacgcg actcgagatg caggatttga ttgtgtcgct
     5221 ttggctgaag cacggtttta ccgttttgct ggtgacgcat gatgtgagcg aagcggtggc
     5281 gatggctgac cgggtgttgt taattgaaga gggaaaaatt ggtctggatt tgacggtgga
     5341 tatcccccgt ccgcgccggt tagggtcggt acgattagca gagctggaag cggaagtgtt
     5401 gcagcgggtg atgcaacgtg ggcactctga acaaccgatt cgacgtcatg gttaatatgc
     5461 cggatgtggc gcacaaaacc agcattttca acgggattgt aggcctgata agacgcgtca
     5521 agcgtcgcat caggcagttg ggcacgactg cctgatgcgg cgtgaacgcc ttatccagcc
     5581 tacaaaacca gcaatttcaa cggacttgta ggcctgataa gacgcgtcaa gcattttggc
     5641 tcaaaagtat caagccagtg ctttggtgat cttctcgtac agatcgccag agagattttc
     5701 cagccctttc aactgttcca gcgccgcgcg cattttctcc tgacgtttgg catcgtaacg
     5761 tttcaggcga atcagcggct caatcaggcg tgaagccacc tgcgggttac ggctgttgag
     5821 gtcggtaagc atttccacca ggaactgata accgctgcca tcttcggcat ggaacgctgc
     5881 cgggttgctg cccgcaaatg cgccaatcaa tgaacgaatg cggttcgggt tgctcatggt
     5941 aaatgagcga tgctgcaaca gaccacgtac cgtctccagc acattcgccg ccgggctggt
     6001 ggcttgcagg ataaaccatt tatccatcac cagaccatcc tgatgccact tgtcgtcgta
     6061 ctcctgcatc agcgcgtcac ggcatggcag ttgtgcagca accgccgcag aaagcgccgc
     6121 cagcgcatcg gtcatattgt tcgcttcgtg gaactgtttg ctcaccagca catcagccag
     6181 atgcgtttca ccaaaagcga ggaagcgcag gcaggcatta cgcagagtgc gttttgcaat
     6241 atcttcatgc tcaacacggt actcgctctg gtaattcgcg ttgtaaatag ccagcagctc
     6301 atctgccagt tcagtcgcca gagtacgagt gagtgcttcg cgtacttcgg caatagcaat
     6361 cgggtcgatg atatcgaaca gttctgccat ttcattgacg gaaggcagcg tcaggatttc
     6421 tgccgccagc gccggatcaa tcttctcatc gagcagcacc gcgcggaaag cgtcagccac
     6481 atgcaccggc agagacaacg gctgcccttg ctgatgacgg gcgacgttca gcttgatgta
     6541 ggttgccagc aaactttgcg ccgcgtccca gcgagagaaa tcattacgcg catgacgcat
     6601 caggaaggtc agttgctgat cgctccactt atattccagt ttcactggcg cagagaattc
     6661 gcacagcagc gcaggcaccg gctggaagta gacattatca aaaacaaagg tctgttccgc
     6721 ctgggtgacg ttcagcacgg aattcaccgg atgaccgcct ttctgcaacg ggatcacttt
     6781 gccttcgttg tcatacagtt cgatggcaaa cggaatatgc agtggttgtt tttctgcctg
     6841 atccggtgta gctggcgtgc gctggctgat ggtcagggtg tactgctcag tttccggatt
     6901 gtagtcgtct ttgacggtca caaccggcgt gccggactgg ctgtaccaac ggcggaaatg
     6961 ggaaagatcg acattcgacg catcttccat cgcctgcaca aaatcgtcac aggtcgctgc
     7021 gctaccatca tgacgctcga aatagagctg catccctttc tggaaatttt cttcgccaag
     7081 cagagtgtgg atcatacgaa tcacttccgc gcccttctcg taaacggtca gggtgtagaa
     7141 gttgttcatt tcaatgacca tatccgggcg aattgggtgc gccatcgggc tggcgtcttc
     7201 tgcaaactgc aatccgcgca tggtgcgcac attattgata cggtttaccg cgcgggaacc
     7261 aaggtcagag ctgaattcct gatcgcggaa gacggttaaa ccttctttca gactgagctg
     7321 gaaccagtcg cggcaggtga cacggttgcc ggtccagtta tggaaatatt catggccgat
     7381 aacgcgttca atatcgaggt aatctttgtc ggtggcggtg tcggtgcggg ccagcacata
     7441 tttggagtta aagatattca gccccttatt ctccattgcg cccatattga agaaatccac
     7501 cgcgacgatc atatagatgt cgaggtcata ctccaggccg aagcgttctt catcccattt
     7561 catggagttt ttcagcgagg tcatcgccca cggcgcgcga tcaaggttgc cacgatcgac
     7621 gtacagctcc agcgctactt cacggccaga gcgggtagtg aaggtatcgc gcagtacatc
     7681 aaaatcacca gccaccaacg caaacaggta acacggtttc gggaacgggt cctgccactg
     7741 tacccaatga cgtccatttt ccagttcacc ttgcgcaacg cggttaccat tggaaagcag
     7801 gaagggatat ttggttttat cggcaataat tttggtggta aaacgcgcca gcacatccgg
     7861 gcggtcgaga taatacgtaa tatggcggaa accttcggct tcacactggg tgcaaagcgc
     7921 atcgcctgac tgataaagcc cttccagcgc agtattcgcc gccgggctaa tttcattaac
     7981 gatcttaagc gtaaaacgct ccggcaaatt actgatgacc agtgcgccct cttcttcttt
     8041 ccaggcggtc cacggctcat cattaatata aacagaaacc agtttgaggt cttcgccgtt
     8101 gagacgaagc ggaacatctg atgcaccatg acggacagcc tggctgaccg cggtaacgac
     8161 cgtcttttgc gcgtccaggt caaaggtcaa gtcaatatca gtaatctggt aatccggcgc
     8221 acgataatcg tgacggtatt tggcttgtgg ctgttgagtc ataaataacc tttagcatct
     8281 tttatagagt ctggtgttca gtctattcct gttgcgtaaa tcgcgctatg cagaatcttc
     8341 atcttttcag gtacaaacgc ctttattact acatttttat aacatacacc gcgttatgcc
     8401 atcgaccaga aaggtggcat atggtgtgat cggggttcaa taaattgcga aacaaggtat
     8461 actccagcag ttcctgaaga tgtttattgt actaaacgct cctgtacgag gacgctactg
     8521 cgcacctatg acacaattcg cttctcctgt tctgcactcg ttgctggata cagatgctta
     8581 taagttgcat atgcagcaag ccgtgtttca tcactattac gatgtgcatg tcgcggcgga
     8641 gtttcgttgc cgaggtgacg atctgctggg tatttatgcc gatgctattc gtgaacaggt
     8701 tcaggcgatg cagcacctgc gcctgcagga tgatgaatat cagtggctct ctgccctgcc
     8761 tttctttaag gccgactatc ttaactggct acgcgagttc cgctttaacc cggaacaagt
     8821 caccgtgtcc aacgataatg gcaagctgga tattcgttta agcggcccgt ggcgtgaagt
     8881 catcctctgg gaagttcctt tgctggcggt tatcagtgaa atggtacatc gctatcgctc
     8941 accgcaggcc gacgttgcgc aagccctcga cacgctggaa aacaaattag tcgacttctc
     9001 ggcattaacc gccggtcttg atatgtcgcg cttccatctg atggattttg gcacccgccg
     9061 ccgtttttct cgcgaagtac aagaaaccat cgtgaaacgt ctgcaccagg aatcctggtt
     9121 cgtgggcacc agcaactacg atctggcgcg tcggctttcc ctcacgccga tgggaacaca
     9181 ggcacacgaa tggttccagg cgcatcagca aatcagcccg gatctagcca acagccagcg
     9241 agctgcactt gctgcctggc tggaagagta tcccgaccaa cttggcattg cgttaaccga
     9301 ctgcatcact atggatgctt tcctgcgaga tttcggtgtc gagttcgcca gtcgctatca
     9361 gggcctgcgc catgactctg gtgacccggt tgaatggggt gaaaaagcca ttgcacatta
     9421 tgaaaagctg ggaattgatc cacagagtaa aacgctggtt ttctctgaca atctggattt
     9481 acgcaaagcg gttgagctat accgccactt ctcttcccgc gtgcaattaa gttttggtat
     9541 tgggactcgc ctgacctgcg atatccccca ggtaaaaccc ctgaatattg tcattaagtt
     9601 ggtagagtgt aacggtaaac cagtggcgaa actttctgac agccctggca aaactatctg
     9661 ccacgataaa gcgtttgttc gggcgctgcg caaagcgttc gaccttccgc atattaaaaa
     9721 agccagttaa tatcatcagg gagctaatcg gctccctttt ttacctttaa ttccgaaatc
     9781 tttcgctgca tttgcgaatt ctgcttgtct gattgcagat gccaggtaac ataggtatcc
     9841 ccccatttac gggtggacaa gtgtttattt tttccgacta ttaacagaga gaatattatg
     9901 agcgttgtgc ctgtagccga cgtactccag ggccgcgtag ccgttgacag cgaagtcacc
     9961 gtgcgcggat gggtacgtac ccgccgagat tcaaaagctg gcatctcctt cctcgccgtt
    10021 tatgacggtt cctgctttga tcctgtacag gctgtcatca ataattctct gcccaattac
    10081 aatgaagacg tcttgcgtct gaccaccggc tgctcggtca ttgtgacggg taaagtcgtg
    10141 gcgtcgccgg gccaggggca acaatttgaa attcaggcca gcaaggttga agttgctggt
    10201 tgggttgaag atccagacac ttacccgatg gcggcaaaac gccacagcat tgagtatctg
    10261 cgtgaagtcg ctcacctgcg tccgcgcaca aacctgattg gtgccgtcgc gcgcgttcgc
    10321 catacgctgg cgcaggcgct gcatcgcttc tttaacgagc agggattctt ctgggtttca
    10381 acgccactga ttaccgcatc tgataccgaa ggtgctggcg aaatgttccg tgtttcaacg
    10441 ctggatctgg aaaacctgcc gcgtaacgat cagggcaaag tagatttcga caaagacttc
    10501 tttggtaaag agtctttcct gaccgtatct ggccagttga acggcgaaac ctacgcttgc
    10561 gcattatcca aaatttatac cttcggcccg actttccgtg ctgaaaactc caacaccagc
    10621 cgtcacctgg cggaattctg gatgctggag ccagaagtgg cgtttgctaa cctgaacgat
    10681 atcgcgggtc tggctgaagc catgctgaaa tatgtcttca aagcggttct cgaagaacgc
    10741 gctgacgaca tgaaattctt cgctgaacgc gtagataaag atgccgtttc acgtctggaa
    10801 cgcttcattg aggccgattt cgcgcaggtg gattacaccg acgcagtgac cattctcgaa
    10861 aactgcggca ggaagtttga aaacccggtt tactggggcg tcgatctctc ttctgagcat
    10921 gagcgttatc tggcggaaga acactttaaa gcaccggtag tggttaaaaa ctatccaaaa
    10981 gatattaaag cgttctatat gcgccttaac gaagacggta aaaccgttgc ggctatggac
    11041 gttctggctc cgggcatcgg tgagatcatt ggtggctccc agcgtgaaga acgtctggac
    11101 gtgctggacg agcgtatgct tgaaatgggc ctgaacaaag aagattactg gtggtatcgc
    11161 gatctgcgtc gctacggtac tgttccgcat tcaggtttcg gtcttggttt tgaacgcctg
    11221 attgcttacg taactggcgt gcaaaacgta cgtgatgtta ttccgttccc acgtactccg
    11281 cgtaacgcca gcttctaatt tagcgtcttc aagagccagc gcccgctggc tcttttttta
    11341 tcctttctgt caagttatct gtttgcaacc ccaccataac gatcatcctg ttacggaata
    11401 ttacgctgca acatttacgc acaaaaaata atctgcattc ttattgcgga ttagtttttt
    11461 cttagctgat agcacaattt tcatactatt tttttggcat tc
//
LOCUS       DAZKHK010000146        11302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKHK010000146 DAZKHK010000000
VERSION     DAZKHK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.147"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(55..1899)
                     /gene="selB"
                     /locus_tag="ACHOS5_004251"
     CDS             complement(55..1899)
                     /gene="selB"
                     /locus_tag="ACHOS5_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP9778974.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(1896..3287)
                     /gene="selA"
                     /locus_tag="ACHOS5_004252"
     CDS             complement(1896..3287)
                     /gene="selA"
                     /locus_tag="ACHOS5_004252"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP9778975.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHAKDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRFQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3385..3993)
                     /locus_tag="ACHOS5_004253"
     CDS             complement(3385..3993)
                     /locus_tag="ACHOS5_004253"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP9778976.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPEMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(4064..5200)
                     /locus_tag="ACHOS5_004254"
     CDS             complement(4064..5200)
                     /locus_tag="ACHOS5_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9778977.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5203..5565)
                     /locus_tag="ACHOS5_004255"
     CDS             complement(5203..5565)
                     /locus_tag="ACHOS5_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9778978.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVAPQGDVPDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6102..8015
                     /gene="mtlA"
                     /locus_tag="ACHOS5_004256"
     CDS             6102..8015
                     /gene="mtlA"
                     /locus_tag="ACHOS5_004256"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP9778979.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8435..9583
                     /gene="mtlD"
                     /locus_tag="ACHOS5_004257"
     CDS             8435..9583
                     /gene="mtlD"
                     /locus_tag="ACHOS5_004257"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9778980.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9583..10170
                     /gene="mtlR"
                     /locus_tag="ACHOS5_004258"
     CDS             9583..10170
                     /gene="mtlR"
                     /locus_tag="ACHOS5_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP9778981.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(10181..10390)
                     /gene="yibT"
                     /locus_tag="ACHOS5_004259"
     CDS             complement(10181..10390)
                     /gene="yibT"
                     /locus_tag="ACHOS5_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP9778982.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            10674..11036
                     /locus_tag="ACHOS5_004260"
     CDS             10674..11036
                     /locus_tag="ACHOS5_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP9778983.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         2743 a         3045 c         2869 g         2645 t
ORIGIN      
        1 acattaataa taaattttgt attaattcat atttattatg aattaatcat ttccttattt
       61 ttccggaaat aataatgcgt cgcgcaataa atgatcattt ccacgacgac gcgtaaagcc
      121 aatacggtca aaatattcca gaatctgaat tgccagcttg cggcctacgc ctaagcgatc
      181 gcggaaatcc gccgcgcagg ttgaaccgca ttcctgatcg agatcgcgga tcatattggc
      241 aaattcgaca atccgatcgt tacggtaata acgatctttg acgatcgcag taattatccc
      301 ttgttgcgcc gcctggcgta gcgtcaggcg cattgcctgc tcgtcggttc ccgtctcttt
      361 tgccaggtca cgcacccacc atggctcgtc accaaacagc ggctctgctt tttgccagat
      421 ggcctgctgc tcttcgctga agcccgcttt gtgatctggc agatgcagcc aaccgtgatg
      481 actgaggatg tcgccgcttt cgcgcatctt ttcaatcagc aacagtacca gcgcttcatc
      541 ttccattggc aacgccatac gtcgcagacg ttcgcgccca ggaccaggtt catcgcgatg
      601 ttgctcatga taagtcgcta atgtgtcgag aattttccgt tgccagcgag cggcaaccgg
      661 cgcattcaac aagctataac cagcctgaat atagccaggc tgttgcagca gttcgcgcat
      721 cccttcaccg ttgagctgcc gtgcccaggc gaaatccgca aggttaaccg cgccgcgttc
      781 cagatgaaca gataacgcat cggcatcgct ctgcgcccgt gcaagagacg ccagccattg
      841 cagatattcc ggcttacgct taccgcgacg cggcgggtta agcatcacca cacgcgcccc
      901 cgccagcgtg ttgcgggccg agatatcgcg cagcactaag cgatcgttat ccgccagcca
      961 cagcggcgtg tcgaacacca gctcggcaag gttatcttcc agtagcgaaa cacgtcccgt
     1021 aacgtggctg gcggcgtggt gaatatgcag cggctgccac tgggtcagcg gtgtatgggt
     1081 ttgaagctcg acaatcaccc gtgtgaacgg ctccggcggc acatcggcaa gcagccagtc
     1141 gccacggtta atctgctctt tttccgcatc acccgcgatg ttaagcgcga tacgctgccc
     1201 ggcatgggca gtttctgttg gctgattttg cgcatgcagc gcacgcacgc gcatcggttt
     1261 atttacacca gtcagccaga gtgaatcgcc tactttcact tccccgctta acgccgtacc
     1321 ggtgacgacc agcccggcac cttttacggt aaatgcgcgg tcgatcgcga ggcggaaact
     1381 atgttggctg gcgtgttcac gttctggtaa ctgaagcaga tgctcgcgca gagcatcaat
     1441 tccccgacct tcggttgctg cggtgataaa cagttttgcc tcagcaaaac cgtattcccg
     1501 cagcacctcc tttacctggc gttcaacctc atcaacacgc gcttcgtcca cgcgatcggc
     1561 tttggtcagc gccactgtca gcatcgggtt accggtcagc tgcaaaatcg ccagatgctc
     1621 acgggtctgt gccatcacac cgtcatcgca cgccaccacc aacagcgcgt gatcgatacc
     1681 accaacgccc gccagcatgt tggaaagaaa cttttcatga ccgggtacgt cgataaaacc
     1741 aggcacgcga ccatccggct gcggccagta ggcatagccg agatcgatgg tcatgccgcg
     1801 ctttttttct tccggcagac ggtcggcatt tacgcccgta atcgcctgca acaaggttgt
     1861 tttgccgtgg tcaacgtgtc cggcagtcgc aataatcatt tcaacaacat ctccaaaaac
     1921 cgttgctcat cttcaaggca gcgtaaatcc agccacaatc gtccgtcata aatacgacca
     1981 atcaccggca ctggcaattc acgccagcgg gcggctaatg actcaaggtg gctaccgcgt
     2041 ccatcatggg gggtaaacgt taatgccgcg ctcggcaggc gatcaaccgg cagcgaacca
     2101 ctgccgatct gcgaaagaca tggcataacc tgtaccgcaa actccgcgcc gtaatgtgcg
     2161 gcaaggggag cctggaaacg ttgtgcctgg atttgaatga cctctgcgct gcgggtaagc
     2221 aggcgcaggg tcggtaattt ttcactcaga gcttcagggt gtaaataaag acgcaacgtg
     2281 gcttccagcg ccgcgagggt cattttatcc gcgcgtaatg cacgcttcag cgggtggctt
     2341 tgcaggcggg cgatcatttc ttttttacca acaataattc ctgcctgcgg cccgcctaac
     2401 agcttgtcgc cggagaagct caccagactg acgcccgccg caatcaactc ctgcggcatt
     2461 ggctctttcg gcaaaccgta ctggctaaga tcgaccagcg agccactgcc taaatcagtc
     2521 accactggaa catccagttc tttgccgagc gccaccagtt ccgcttcatc tatcgctttg
     2581 gtgaacccct gaatgctgta gttactggta tgtactttca tcaacagtgc ggtattttca
     2641 ttcaccgcct gacgataatc cttcgcgtgc gtgcggttgg tggtccctac ttcgtgtagg
     2701 gtgcatcctg cctgacgcat aacatcggga atacgaaacg cgccgccaat ctccaccagt
     2761 tcgccgcgag aaaccaccac ctctttcccg ctggcagtgg ccgccaacat caataacacc
     2821 gccgccgcat tgttattaac gatacaggca tcttccgccc ccgtaatacg gcacagcagc
     2881 tgcgccagcg cccgatcgcg atgtccgcgt ccggcgtcgt ccagatcgta ctcgagggtc
     2941 actggcgaac gcatagcctt cgtaacggct tccaccgcgg cttccgcctg taaagctcgc
     3001 ccaaggttgg tatgcagcac ggttcccgtc aggttgatca ccggacgcag cgcgctttgc
     3061 gcctctttcg tcaaccgggc atcgacttct tgcgcccagt tttcacacca cgcgggcagc
     3121 gtctggctgt cacgaatcac ttctcgcgct tcgtcaagca tctgacgcag caattccacc
     3181 acgcgggtgt gaccataagt atcacgcaaa gaaaggaagg agctatcgcg caataagcga
     3241 tcaatagccg gaagttgact atagagggaa cgcgtttcgg ttgtcatagg aaacctggct
     3301 gatcaaggcc ctctcacacg gagaagggcg tttaacataa ccacggattg taacgtgaga
     3361 tgggtcagga ggacatatcg cgcatcaagc ctttggcggt tcggtgcgag caaaactttc
     3421 gcggctaaac aggttttcga ccagtttgac taagtgtggg cggtcgacac accagcctgg
     3481 cgcaacgcgg cggaaattga gatatccgac ggcacaggca atggcgatag tcgccagatt
     3541 gaccgtatcg gttttgagcg tgccatcgac cagatatcct tccagcacat ccagactgcg
     3601 gttgattttc tcacgctgac gtaacaattc atcttcagac tgctgcgccg ccggacgcgc
     3661 ctgttcacgc accgatacca gcccggcatc cataatgcca tccgccagtg cctcaatttt
     3721 gcgcacccgc aacgactcca gcggatcacg cggcaacatc tccggagcaa cattcattaa
     3781 ttcaatatat tcagcgatga tcggcgaatc aaaccagcat tcgccctctt cggtcaccag
     3841 caccggcact tttcctaacg ggttaaattg cgccacgccg ttatccgcgt tatagggcag
     3901 ttcattaata aattcgaacg ttatgccctt ttctaacaac agaatagaaa gtttgcgtac
     3961 aaacgggctg gtgtagctac cgacgagttt catgccgagt cctttgtgcg aggaaaaata
     4021 tcagtatggc tcaggctcgc gcaaaacaca ggtgatacaa gtctcaatga tccaaataaa
     4081 gataatgcat ccaactggtc attcttagca gcactttccg catcaccgaa acatggctga
     4141 aatggtcgga gtaaactgcc acctgggcgt agatgccgtc gggtaaggca tcgatatcag
     4201 cgttagggtc cagttcaatg gttcccagca caccgtccgt gccgggcacg accgttaatg
     4261 attgcaatac cccctgcgcc tgataagaac cgcctggcac gacaggtaaa atactggtca
     4321 gtttgccgtg aaacacctgc ccaggtagcg cgttaaacac cacttccgca tcatcgccgg
     4381 gtttcagacg taacagcgag ttttgccgaa attgggcgac aatttgccgt ttttgctcgg
     4441 ggatgaagac catcaccgga cgcagcggca aggcagctgc gtatgtacct gggcggatca
     4501 gtacctgagt gacgtagcca ttgctcggtg cgcgaatgac agtctgctca aggttatatt
     4561 ttgcttcagt aagttgcgct cttaagctca caatctgcga ttgctcgccg ttaaccatac
     4621 tgtcgagctg gctctggatc tgcgcctgct ccgccaccga gcctttcacc agcgcatcct
     4681 gcgcgaggaa attttgccgc gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg
     4741 cctggctgcc tttcagataa cgttgataat ttttaaacag acggtcgcgc tccgctgaaa
     4801 cctgggtggt gttggcctgc gcttcagtga gctgcgcgcg cagcgtcttt atattatgcg
     4861 tcgccgtcat caggtcagcc tgaagcctgt caactcgcgc ctggtaacga accgggtcga
     4921 gcttaaaaag cacctcgccc ttctgaataa gctgattatt tttgtcggtg acttcagtaa
     4981 caattcccgt cacctgtggc gtgatgggga tcgctatcac tgccttttgc gcggtaaaag
     5041 tgtaagggtg gttgtagttc atcaacaaaa tcaaaccact caccagaaac acgcctccca
     5101 gcgccgccgt cgccagcgtc cactgattca ccggaatgcg gaagatttta aagaccgccc
     5161 acgccagcgc cacgtaagtt aaaacaatca atagatccat aattagatct ccggaaacgt
     5221 ggaatagtca gtctttttct cggcggcgag ttggtgctcc agccgggaaa tccggtcaga
     5281 aagtgcggcg atttccgggt ctggcacgtc gccttgcgga gcaacatgtg actgcatgcc
     5341 ccagccgcgc tccggttgat agagcgtcgc ccagatccac aggaacggcc agataacatg
     5401 cagagtaaac aggctcaccc agcccgccgt atgaatagcg tcggcatggg gatggttgcg
     5461 ctttttggca atcagataag ggatgtcatg tatcgcgatg attccataaa aaatcaccag
     5521 aaagacgaag atcagcactc ccaacgcgaa atagtttaga aacatatctg cctcggattc
     5581 acgtttatca gtgttgtttt ttggctggca gccagaaggg agtcaggctg atattttgac
     5641 aataatccgg gttcgcgatt ctcgccataa caccaaagaa taattttaag aggtgatgag
     5701 ttgcttagtt acataacgat ttcatgacga aggcataaca tgctgtagat cacatcaggt
     5761 gaacgccgta agaaaatatc ttgtgattca gatcacaaag attcaacaaa ccatcaaaat
     5821 aaaaatgtga cataactcac atttaaatgc catttttagc gaaaatcgcc gccttgttgc
     5881 ttttttacac aagcattttg tgatgagcat cacgtcaatt acctctctac cccctacatt
     5941 tatgtgactg atatcacaca aaaggccgcc gactggacag ttaaccgatt cagtgccaga
     6001 tttcgcagta tctacaaggt ccggctacct ctgccgccac attaacaaaa aacctcgggc
     6061 ttccagcctg cgcgacagca aacataagaa ggggtgtttt tatgtcatcc gatattaaga
     6121 tcaaagtgca aagctttggt cgtttcctca gcaacatggt gatgccaaat atcggcgcgt
     6181 ttatcgcgtg gggtatcatc accgcgttat ttattccaac agggtggtta ccgaacgaaa
     6241 cgctggcgaa gctggtcggg ccgatgatca cttatctcct gccgctgctg atcggttata
     6301 ccggtggtaa gctggtaggc ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg
     6361 gcgttatcgt cggcgcagac atgccgatgt tcctcggctc tatgattgca ggtccgctgg
     6421 gcggctggtg cattaagcac ttcgaccgct gggtagacgg taagatcaaa tccggttttg
     6481 aaatgctggt gaataacttc tccgcaggca tcatcgggat gatcctcgct attctggcat
     6541 ttctcggcat tggcccggtt gttgaagccc tgtctaaaat gctggcggcg ggcgttaact
     6601 tcatggttgt ccatgacatg ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc
     6661 tgttcctcaa caacgccatt aaccacggta tcttctcgcc gctgggtatt cagcagtccc
     6721 atgaactggg taaatccatc ttcttcctga ttgaagctaa cccaggtccg ggtatgggcg
     6781 tgctgctggc gtacatgttc tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg
     6841 caatcatcca cttcctgggg ggtatccacg aaatctactt cccgtatgtg ctgatgaatc
     6901 cgcgtctgat tctggccgtt atcctcggcg gtatgactgg cgtgttcacg ctgactatcc
     6961 tgggcggtgg tctggtttct ccggcatctc cgggttctat ccttgctgta ctggcgatga
     7021 cgccaaaagg tgcttacttc gctaacatcg cgggtgtgtg tgcggcgatg gcagtctcct
     7081 tcgttgtctc tgccattctg ctgaaaacca gcaaagtgaa agaagaagat gatattgaag
     7141 ctgctgcacg tcgcgttcag gaaatgaaag ctgagtctaa aggcgcatct ccgctgtctg
     7201 cgggcgatgt gactaacgac ctgagccacg tacgtaaaat catcgttgcc tgtgatgccg
     7261 gtatgggttc cagtgcgatg ggtgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc
     7321 tgtcgcagat ttcggtcact aacagcgcga tcaacaacct gccgccagat gtggacctgg
     7381 tcatcactca ccgtgacctg accgaacgcg ctatgcgcca ggttccgcag gcacagcata
     7441 tttcgctgac caacttcctc gacagcggcc tgtacaccag cctgaccgaa cgtctggttg
     7501 ctgcccagcg ccatactgaa aacgaagtga aagtgaaaga cagtctgaaa gacagctttg
     7561 acgattccag tgctaatctg ttcaaactgg gcgcggagaa catcttcctc ggtcgcaaag
     7621 cggccaccaa agaagaagcg attcgttttg ctggcgagca gctggtgaaa ggcggttacg
     7681 ttgagccgga atacgttcag gcgatgttgg atcgtgaaaa actgaccccg acttatctgg
     7741 gtgagtctat cgcggtgcca cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg
     7801 gcgtcgtgtt ctgccagtac ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg
     7861 cccgtctggt gattggtatt gctgcccgta acaacgagca cattcaggtg atcaccagcc
     7921 tgaccaacgc actggatgat gagtccgtta tcgagcgtct ggcgcacacc accagcgtgg
     7981 atgaagtgct ggaactgctg gcaggtcgta agtaatccat tcccaccctc tccgcatgaa
     8041 gaaggtgggg ctaattgcct gatgcgcttc gcttatcagg cctacaacat aaagcacaat
     8101 ttgttttgtc ggtcggataa ggtgtttacg ccgcatccgg cagtcggtac acaatgcctg
     8161 atgcgacgct gacgcgtctt atcaggccta caggtcagtg ccaaacacgt aggccggata
     8221 aggcgtttac gccgcatccg gcagtcggtg cgcaatacct gatgcgacgc tgacgcgtct
     8281 tatcaggcct acaggtcagt gccaaacacg taggccggat aaggcgttta cgccgcatcc
     8341 ggcaaaaagc ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc
     8401 ctctcgggta aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg
     8461 taatatcggt cgtggcttta tcggtaaact gctggcggac gcgggtatcc aactgacgtt
     8521 tgccgatgta aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca
     8581 tgtggtcggc gaaaccgagc aggtggatac cgtttctggc gtcgatgccg tcagcagcat
     8641 tggcgatgat gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc
     8701 tgtcgtgctg gaacgtattg ctccggccat cgccaaaggg ctggtgaaac gtaaagaaca
     8761 aggtaatgaa tccccactga acatcatcgc ctgtgaaaac atggtccgcg gcaccacgca
     8821 gctgaaaggc catgtgatga acgccctgcc ggaagatgcc aaagcgtggg tagaagaaca
     8881 cgttggcttt gttgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa
     8941 cgatccgctg gaagtgacgg tagaaacctt cagcgaatgg attgtcgata aaacgcagtt
     9001 caaaggcacg ctgccaaaca tccctggcat ggaattaacc gacaatctga tggcatttgt
     9061 cgaacgtaaa ctcttcaccc tgaatacagg tcatgctata accgcgtacc tcggaaaact
     9121 ggccggtcat cagaccattc gtgacgcaat tctcgacgag aaaatccgcg cggtggtaaa
     9181 aggggcaatg gaagaaagcg gcgcggtatt gatcaagcgc tacggctttg atgctgacaa
     9241 acatgcggca tacatccaga aaatcctcgg tcgtttcgag aacccgtacc tgaaagatga
     9301 tgtagaacgc gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa
     9361 gccactgctc ggaaccctgg aatatggtct gccacataaa aacctgattg aaggtattgc
     9421 cgctgcaatg cacttccgca gtgaagatga tccgcaggct caggaactgg cagcgctgat
     9481 tgccgacaaa ggcccacaag ccgcgctggc acagatttcc ggtcttgatg ccaacagcga
     9541 ggttgtatcc gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag
     9601 gataccctgc gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag
     9661 gcctttgaaa accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg
     9721 atcaccgccg tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc
     9781 aaagacgatt acgcggtgaa gtatgctgta gaaccgttac tcgacggcga tggtccgctg
     9841 ggcgatcttt ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa
     9901 tacgaagatg cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag
     9961 tacgctttta ccgacgacga aatacttgga ccctttggtg aactgcattg cgtggcggcg
    10021 ttaccaccgc cgccacagtt tgaacctgca gactccagtt tgtatgcaat gcaaattcag
    10081 cgttaccaac aggctgtacg atcaacaatg gtcctttcac tgacagagct gatttcaaaa
    10141 atcagcttaa aaaaagcctt tcaaaagtaa gtactttcgc ttacttctcc tctacctgtt
    10201 tcggacgata aagctgacga taatactcca gacgttgtag atataacggc acgctttcat
    10261 caggtattcc ggacggaatc gcgttacggg gaggtagttt tttcagatac tcccggaacg
    10321 cctggctgga tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta
    10381 atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt
    10441 agtttagatc ccgatataaa tgacattaag gaaaacgtgc tcaatcctca ataatcacct
    10501 gcttctgttg actatctttt ttctttaaat ttcttaacta atcaatgtat tgggtgattt
    10561 tttaacgcat taatggttac aatctggaac tattacaatg ccatcagttg tgaacaaagc
    10621 acctggtcgc gcatactaga ggctataaat ttatctctat cagaagtcat cacatgaaag
    10681 aagtcgaaaa aaacgaaatc aaacgtctca gcgatcgtct ggacgccatc cgtcaccagc
    10741 aggccgatct atcgctggtt gaagccgcag acaaatatgc cgaactggaa aaagagaaag
    10801 ccacgctgga agcagaaatt gctcgcctgc gcgaagttca cagccagaaa ctcagcaaag
    10861 aagcgcagaa actgatgaaa atgccgttcc agcgtgcaat caccaaaaaa gagcaggctg
    10921 atatgggcaa gctgaaaaaa agtgttcgcg gactggtggt tgtgcaccca atgaccgcgc
    10981 tgggccgcga aatgggcctg caagagatga cagggttctc aaagactgcg ttttaagttc
    11041 ctttctcgtc gctgtaatca cagcctctca ccatttactc gctcctgctg gcacatgcag
    11101 gagcctgttt cagagatcga atctttataa cgttcttaac attatatatt caactccacc
    11161 tacacttctg agcgatcgta gcaggctaaa ttctgaaaat caggtacaca ttaaagaatg
    11221 atgtatcaat atcaacaatg aattgccgca tatcaatgct tgaaacataa ttaattattt
    11281 aaatttttca atcagcttcg ca
//
LOCUS       DAZKHK010000147        11226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKHK010000147 DAZKHK010000000
VERSION     DAZKHK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.148"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..130
                     /locus_tag="ACHOS5_004261"
     CDS             <1..130
                     /locus_tag="ACHOS5_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9778984.1"
                     /translation="YYLGYDRFLLIFFTDYSYFMDRITLPSTDTEKGKGKWKIITA"
     gene            109..213
                     /gene="speFL"
                     /locus_tag="ACHOS5_004262"
     CDS             109..213
                     /gene="speFL"
                     /locus_tag="ACHOS5_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP9778985.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            523..2721
                     /gene="speF"
                     /locus_tag="ACHOS5_004263"
     CDS             523..2721
                     /gene="speF"
                     /locus_tag="ACHOS5_004263"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP9778986.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2718..4037
                     /gene="potE"
                     /locus_tag="ACHOS5_004264"
     CDS             2718..4037
                     /gene="potE"
                     /locus_tag="ACHOS5_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP9778987.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4094..5734)
                     /gene="pgm"
                     /locus_tag="ACHOS5_004265"
     CDS             complement(4094..5734)
                     /gene="pgm"
                     /locus_tag="ACHOS5_004265"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP9778988.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5760..6305)
                     /gene="seqA"
                     /locus_tag="ACHOS5_004266"
     CDS             complement(5760..6305)
                     /gene="seqA"
                     /locus_tag="ACHOS5_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP9778989.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6490..7254
                     /gene="ybfF"
                     /locus_tag="ACHOS5_004267"
     CDS             6490..7254
                     /gene="ybfF"
                     /locus_tag="ACHOS5_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9778990.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7394..7687
                     /gene="ybfE"
                     /locus_tag="ACHOS5_004268"
     CDS             7394..7687
                     /gene="ybfE"
                     /locus_tag="ACHOS5_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP9778991.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7827..8357
                     /gene="fldA"
                     /locus_tag="ACHOS5_004269"
     CDS             7827..8357
                     /gene="fldA"
                     /locus_tag="ACHOS5_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP9778992.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8567..8653
                     /gene="uof"
                     /locus_tag="ACHOS5_004270"
     CDS             8567..8653
                     /gene="uof"
                     /locus_tag="ACHOS5_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP9778993.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8646..9092
                     /gene="fur"
                     /locus_tag="ACHOS5_004271"
     CDS             8646..9092
                     /gene="fur"
                     /locus_tag="ACHOS5_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP9778994.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9176..9502)
                     /gene="chiQ"
                     /locus_tag="ACHOS5_004272"
     CDS             complement(9176..9502)
                     /gene="chiQ"
                     /locus_tag="ACHOS5_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP9778995.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9552..10958)
                     /gene="chiP"
                     /locus_tag="ACHOS5_004273"
     CDS             complement(9552..10958)
                     /gene="chiP"
                     /locus_tag="ACHOS5_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP9778996.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2742 a         2779 c         2779 g         2926 t
ORIGIN      
        1 ttattatttg ggatatgatc gttttttgtt gatcttcttc acagattata gctatttcat
       61 ggatagaata accctacctt caactgacac agaaaaaggt aaaggtaaat ggaaaataat
      121 aaccgcttaa tgcctcatat aaggcggaca acccatatca tgaagtttgc ccatcgtaat
      181 agttttgact ttcatttctt taatgcccgc tagtcttctg actaaagggc acccctgcgt
      241 acaggtctcc ctgactttaa gcattacagg ttaatacctg tatccctcgg tgctcatata
      301 ctgctaaccc tttcaaactc taaataattc gagtcgcagc acatgcaact tgaagtatga
      361 cgagtatagc cagttaccgg gctggtctgg gttattgcat ctgcaaaaag caaactactg
      421 atttatttat cagcggtggg gttttgcttt tttccggcgt gatcaatttc tcctttgaga
      481 aattgaggac ctgctattac ctgaaataaa gagatgaaca aaatgtcaga attaaaaatt
      541 gccgttagcc gttcttgccc ggattgtttt tccactcatc gtgaatgcgt gaatattgac
      601 aaaagtaatt atattgacgt tgccgctatt attttatcag tcaatgatgt tgaacgtgga
      661 aaactcgatg aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat
      721 gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa
      781 tcacggaaag agttttatgg acgtcagtta gaaaccgctg ccagccatta tgaaacgcaa
      841 ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt
      901 gactgcccgg gtcatcaggg aggtgagttt ttccgtcgcc atccggcagg caatcagttc
      961 gtggaatatt ttggcgaaat gctcttccgc tccgacttgt gtaatgccga cgttgcaatg
     1021 ggtgatcttc tgattcatga aggtgcgcca tgcattgcac agcaacatgc ggctaaagtg
     1081 tttaatgccg ataaaacgta ctttgtttta aacggcacct cctcctccaa caaagtggtt
     1141 ttaaacgccc tgctgacacc gggtgatctg gtgctgtttg accgcaataa ccataagtct
     1201 aaccatcacg gggccttgct acaggctggt gcaacaccgg tttatctgga aacagcacgt
     1261 aacccgtatg gttttatcgg tggcattgat gcgcactgct tcgaagaaag ttatctgcgt
     1321 gaactgatta cagaagttgc accgcaacgg gcaaaagaag cgcgtccgtt ccgcctcgct
     1381 gtgattcagt taggcaccta cgacggtact atttataacg cccgccaggt ggtggataaa
     1441 atcggtcatc tgtgtgacta catcctgttt gactcagcat gggtcggcta tgaacagttt
     1501 attccgatga tggcggactg ttcgccgctg ctgctggagc ttaatgagaa cgatccgggt
     1561 attctggtta cgcaatctgt gcataaacaa caggctggtt tttctcagac ttcacaaatt
     1621 cataaaaaag acagccatat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
     1681 aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
     1741 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggtt
     1801 aacggtatta atgcccgcaa attgatcctc gataactgcc agcacattcg cccgttcgtt
     1861 cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
     1921 ctgcgcttct ttaaatttgt acccggtgag cactggcatt cttttgaagg ctacgcagag
     1981 aaccaatact ttgtcgatcc gtgcaaactg ttgctgacaa ctccaggtat tgatgcacgt
     2041 aacggcgaat atgaagcgtt cggtgtacct gcaacgattc ttgctaactt cctgcgcgaa
     2101 aatggcgtag tgccggaaaa atgcgatctt aactccatcc tcttcctgct gactccggca
     2161 gaagatatgg ccaaactgca gcaacttgtt gccctgctgg tacgcttcga aaaactactg
     2221 gaagctgatg cgcctctggc ggaagtactg ccttccatct acaaacagca tgaagagcgc
     2281 tacgccggtt ataccctgcg tcagttgtgc caggaaatgc atgatttgta tgcccgtcac
     2341 aacgtgaaac aactgcaaaa agagatgttc cgtaaggaac acttccctcg cgtcagcatg
     2401 aatccgcaag aagccaacta cgcctactta cgcggtgaag tggagctggt tcgtctgccg
     2461 gatgcagaag gccgtatcgc tgccgaaggt gcgcttcctt atcctccggg tgtgctgtgt
     2521 gttgttcctg gtgaaatctg gggtggtgct gttttgcgtt acttcagcgc tctggaagaa
     2581 ggcatcaacc tgctgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
     2641 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcaccctg
     2701 ttgaaagggg aaaaattatg agtcaggcga aatcgaacaa aatgggtgtc gttcagttaa
     2761 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
     2821 ccgaagtcgg gacaatctca attatctcct ggctggtgac agccgtaggc tcaatggcac
     2881 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggaggt atgggcggtt
     2941 atgccgagta tgctttcggt aaatccggta actttatggc gaactatacc tacggcgtct
     3001 cgctgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
     3061 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
     3121 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
     3181 ggggggtcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
     3241 cactttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgcg gtaggttctt
     3301 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
     3361 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
     3421 cggtgattta tatcgtctcc accaacgtga ttgccgggat tgtgccaaat atggagctgg
     3481 caaattcaac ggcaccattt ggtctggcct tcgcgcagat gttcacgccg gaagtgggta
     3541 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
     3601 ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa attttctccc
     3661 gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt atccagagcg
     3721 ggttggcgct gatgacgatt agcccgtcgc tgaacagtca gttcaacgtg ctggttaacc
     3781 tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta gtcattattc
     3841 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
     3901 gcgcgatgta cagcttttat gcgctgtact catccgggga agaagccatg ctatacggtt
     3961 ccatcgtgac cttcctcggt tggacactgt atggtctggt ctcaccacgc tttgaactga
     4021 aaaataaaca cggttaatta agcaaagcaa aaaagggcga tcttccgacc gccctttttc
     4081 ttatttgata tgtttacgcg tttttcagaa cttcgctaac aatctcaacc gcttctttct
     4141 caatctgctt gcgatgttct tcaccaagga agctttcgca gtagatctta tatgcgtctt
     4201 ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt catcactttc agaccgccga
     4261 tagaagcgcc attacccggt gccgccgtca gacgcgcggt gatcgggtca cctgccaggg
     4321 tgctggcgct caccatttcc ggagacagct tagacaacgc cgctttttgt gcggaagtcg
     4381 ctgctgcctg caaacggtta tagctcggcg caccaaagcg ttttgccagt tcgttatagt
     4441 gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc cgccagcaga cacataatga
     4501 tgccgtcttt atcggtggac cacggcgtgc cgtcgaaacg caggaaggaa gcgcctgcac
     4561 tctcttcgcc gccaaagccg aagctgccat cgaacaaacc atcaacaaac catttgaaac
     4621 ctaccggtac ttctaccagc ttacggccca aatcattgac cacacggtcg atcatcgcag
     4681 atgaaaccag cgttttaccg acagcaacat ctttgcccca ctgcggacga tgctggaaca
     4741 ggtagttgat cgccaccgcc aggtagtggt tcggattcat caaacctgcc ggagtgacga
     4801 taccgtgacg gtcataatcc gggtcgttag caaacgccag atcgaactta tcacgcagtg
     4861 ccagcaggcc cgccatcgca cactcggagg agcagtccat acggatcgca ccgtctttat
     4921 cgaggtgcat aaagcggaag gtttgatcga cctgatcgtt aacgatggtc aggttgaggt
     4981 tgtaatactc gccaatgcgc ttccagtatt cgataccgga accacccagc ggatcaacgc
     5041 ccagcgtcag gcccgctttc tgaatcgccg ccatatcaac gatatcggcc agcccttcca
     5101 caaacggctg caccagatcc tgctctttca catgaccgga tgccatcgct tcgtcgaggg
     5161 agatacgctt cacgcctttc aggccatcag ccagcagtgc gttggctctg tcttccacca
     5221 ctttagtgac gttggtatca gccgggccac catttggcgg attgtacttg ataccaccat
     5281 cttccggcgg gttatgggac ggtgtaatca cgataccgtc tgccagcggg ccaccttttt
     5341 tattgtgaac cagaatggca ttggaaatgg caggtgtcgg agtgaagcca ttgttttcct
     5401 gcacaatgac atcaacgccg ttcgctgcca gcacttccag tacggaaatg aacgcaggct
     5461 cggacagggc gtgagtatct ttacccacat agcaagggcc agtgatgccg tttttcgcac
     5521 gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg ctcgttaaag ctgtggcgcg
     5581 ctgcactgcc acggtgaccg gaagtaccga atttcaccgc gtgctccgca ttccctgctt
     5641 ctggtttcag tacataatat tgcgccgtca gttgggcgac gttaatcaaa tcactctgtt
     5701 gtgcaggttg gcctgcacga ttgtggattg ccattgcttt gtcctttgtc tgcaacgttt
     5761 tagatagttc cgcaaacctt ctcaatcaat tccgccggga attgcatcga ctgcatgatg
     5821 tgttcgatca tgctgcattt acggccggtg ttggtgttgg tgatcaccca atacggcgta
     5881 ccaggcacat gtttcggctt ggtctgatta ccatttttca gcagcgtttg ttcatctgcc
     5941 gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg cttcggcaaa cgcctgggcg
     6001 tcaagagaat atagtgtaga caacagcagc ataaagcgat tgaccgctcg cttttgctct
     6061 gcgtattcgt ccgaaagcag aagttcacgc attgcgcgaa ccttgtcttt aatcgttttg
     6121 accggcttcg cttcgacgat agcaggtgac gcaacgcgaa cctctttcgt caccggagca
     6181 gcaggctgtg atgcggcgga aaatttcaac atacgccgta aaatgtcgga tgcgctctcg
     6241 ccgatatgct tagtgtggct ggcaatatag ctgtagagtt catcatcaac ttcaatcgtt
     6301 ttcatcttaa tccagtgcag tgtcttagct gaatacaagt cgccaggagt atatgaataa
     6361 atcccggcag cggatagcgt caagcctggc ttgcagaaaa actcggaata aaccttattt
     6421 gttgcagccc tgtggcacaa tggtcaacat aataccctaa cccgacaacg cgaaaaaaag
     6481 aactttgcca tgaaattgaa tatccgcgcg caaactgcac aaaaccagca caataattct
     6541 cccatcgttc ttgtccatgg tctatttggc agcctcgaca accttggcgt actggctcgc
     6601 gatctggtaa acgatcacaa tatcatccag gttgatatgc gtaaccacgg tctttcaccg
     6661 agagatccgg taatgaatta cccggcgatg gcgcaggatc ttgttgatac cctggatgca
     6721 cagcagatcg acaaagcaac atttatcggt cactccatgg gtggtaaagc ggtaatggct
     6781 cttactgcac tagcccccga tcgcattgac aagctggtgg cgattgatat tgcgccggtc
     6841 gattatcacg ttcgccgtca tgatgagatt ttcgcggcca tcaacgcagt cagtgaatca
     6901 gacgcacaaa cacgccagca agcagcggca ataatgcgcc agcatcttaa tgaagaaggg
     6961 gtgattcagt ttctgttgaa atcttttgtt gatggggagt ggcgctttaa cgtgccagta
     7021 ttgtgggatc agtatccgca tattgtgggt tgggagaaaa tcccggcatg ggatcatcct
     7081 accctattta tccctggcgg caattctccg tatgttagcg agcagtaccg tgatgattta
     7141 ctggcacaat ttccacaggc tcgggctcat gtgattgcag gcgcgggtca ctgggttcat
     7201 gctgaaaaac cggatgcggt attacgcgct atccgtcgct atctcaatga ttaacgcaac
     7261 tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg acgttggcgt gactcatcgc
     7321 gctgatgtat tatggcgcgc tatccatccg ggcaggcgcc tggctgattg tttcccccga
     7381 agtcaccaag atcatggcca aagaacaaac ggaccgtacg acattagatc tgttcgcgca
     7441 cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg cgcgatgagc agctgcgtat
     7501 taataaacgc aaccaactaa aacgcgacaa agtacgtggc cttaagcgtg tcgaactgaa
     7561 gctgaacgcg gaagctgtcg aggcactgaa cgagctggcg gaatcgcgca atatgagccg
     7621 tagcgagctg atcgaagaga tgttaatgca gcaactggcg gcgctgcgta gtcagggcat
     7681 cgtttaaatt tccactttca tgtagcacag tgtgcagtcc tgctcgtttg ctgattccgc
     7741 aagactgcct gttctgctat gattgccttt atccgtgggc aattttccac ccccatttca
     7801 ataagtttca agaggttatt tcactcatgg ctatcactgg catctttttc ggcagcgaca
     7861 ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca gcttggtaaa gacgttgccg
     7921 atgtccatga cattgcaaaa agcagcaaag aagatctgga agcatatgac attctgctgc
     7981 tgggcatccc aacctggtat tacggcgaag cgcagtgtga ctgggatgac ttcttcccga
     8041 ctctcgaaga gattgatttc aatggtaaac tggttgcgct gtttggttgt ggcgaccagg
     8101 aagattacgc agaatacttc tgcgatgcat taggcaccat ccgcgacatc attgaaccgc
     8161 gcggcgcaac tatcgttggt cactggccaa ccgcgggcta ccacttcgaa gcgtcaaaag
     8221 gtctggctga tgatgaccac tttgtcggtc tggctatcga cgaagaccgt cagccggaac
     8281 tgaccgctga acgtgtagaa aaatgggtta aacaaatttc tgaagagctg catctcgacg
     8341 aaattctcaa tgcctgatgt gatgcggcgt agactcatgt ctacgccgta ttaatagata
     8401 atgccaatca aaataattgc tacaaatttg taacttttgc tgttgtacct gtacaatgtc
     8461 ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt gccacgtttt tattaacaat
     8521 atttgccagg gacttgtggt tttcatttag gcgtggcaat tctataatga tacgcattat
     8581 ctcaagagca aattctgtca cttcttctaa tgaagtgaac cgcttagtaa caggacagat
     8641 tccgcatgac tgataacaat accgccctaa agaaagctgg cctgaaagta acgcttcctc
     8701 gtttaaaaat cctggaagtt cttcaggagc cggacaacca tcacgtcagt gcggaagatt
     8761 tatacaaacg tctgatcgat atgggtgaag aaattggtct ggctacggta tatcgcgtac
     8821 tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa ttttgaaggc ggtaaatccg
     8881 tatttgaact gacacagcaa catcaccacg atcacctgat ctgcctcgac tgcggcaagg
     8941 taatcgaatt tagtgatgat tccatcgaag cgcgtcagcg tgaaattgcc gcaaaacatg
     9001 gcattcgcct gactaaccac agtctctatc tttacggtca ctgtgccgaa ggcgattgcc
     9061 gcgaagatga gcacgcgcac gaaggcaaat aagccagcct gaacgagaaa agccaacctg
     9121 cgggttggct tttttataca cgggaaataa gaaaagcgag agttacagct ctcacttatt
     9181 tgttattact gcgaatttcc tgccagacct tatcacaatc ggctttcacc gcctgatcat
     9241 ttccggtttg cgttgcgcgc agacactgct gataatccag tacgcggaca ctttcctggt
     9301 cggcaaactg ctgatgttgt ttctctttct tcagcacgtt taacacgctc tggcaggctt
     9361 caattttttc tggcgaacct tgtgcggtgt tgatacaggc gctgtatgcc tctttcagac
     9421 gactgtcttc ctgtggcgcg gttgattgcg cacaagctac cagccccgat gccattatgg
     9481 cgatgagaat cagttttttc atagcaaatt cctcaaacgt gcggcaggca caaaacctgc
     9541 cgcgtcgggc atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca
     9601 tcctggaaga tgttgccgta accaccgccc cagcttggga tgtcggagtg gttgtcgtat
     9661 tcggtgaagt gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc
     9721 gcatccaggc tgtaggcaga ctcttcaata gtccggtttt tgtcgtagta cgcatccgga
     9781 ttgctctgcc aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg
     9841 aagcctggaa gattccagtt tttcaggtca tacatcgcac cgaagaagac tgctttttcg
     9901 ccgttagcgt tgaagtcgga acggttatcc caccagatat ccaggcgacc gttcgaggaa
     9961 gcgtaggttg gagtcatacg ttgcaggaag tatccctgct gaccgtcagc cttaacccag
    10021 gtaccttcga ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcctgc
    10081 agccaggcgg tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgcgta
    10141 ccatagaact ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct
    10201 ttggcaaaat attgatcgat atacccttcc gcctgaccaa acgccgcttc cagtacgaag
    10261 ttatttttga agtcgtactt cgccccaagg gagtgtagat aatcaacttt ggtggtttta
    10321 tcgttctgat aaaactcatc catttccaga tgccacggtg ctttgtattc gttggtccac
    10381 atgtaggaga aactcaacgc accagcatcg ccgtaatcaa aattcgcccc ggcttccgca
    10441 ccctgataag tacccggcat aaagctccag tgtggcgcta acagcgtctg accggttggc
    10501 tgaatataac ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc tgctttatac
    10561 aggcttatac cgcttttatc gccggaccaa tcttcgtcat aggctttatt actttttgaa
    10621 aacgcgattt cgttcgggtg agagctgtcg ccgttttccg ccatttcaat cgccgtaaac
    10681 gcggcaatat caaggccgaa catatcagca gcataaccag actgaaaatc gaggttggcg
    10741 ttccaggtgg agtgagaaag gttggttttg tatttatcgc cgtcggtaac atctttacgg
    10801 tcgcgttcac gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct
    10861 tcggcgcgag cctccggaat tgccataaag cccgacatag ctgtaacacc ggcgatagcc
    10921 agcgccagcg tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat
    10981 aaattgctgc accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga
    11041 aaagtctcgc tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc
    11101 tgtaaccaga atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg
    11161 cttaagtttg cagttgaaag gcttgcagaa gaataaaagc gtgaatttcc agcaagatga
    11221 aaacgg
//
LOCUS       DAZKHK010000148        11025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKHK010000148 DAZKHK010000000
VERSION     DAZKHK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.149"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(214..1056)
                     /gene="purU"
                     /locus_tag="ACHOS5_004274"
     CDS             complement(214..1056)
                     /gene="purU"
                     /locus_tag="ACHOS5_004274"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP9778997.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1106..1564)
                     /gene="ychJ"
                     /locus_tag="ACHOS5_004275"
     CDS             complement(1106..1564)
                     /gene="ychJ"
                     /locus_tag="ACHOS5_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP9778998.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGATALRVELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDDTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1677..2582
                     /gene="rssA"
                     /locus_tag="ACHOS5_004276"
     CDS             1677..2582
                     /gene="rssA"
                     /locus_tag="ACHOS5_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP9778999.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2674..3687
                     /gene="rssB"
                     /locus_tag="ACHOS5_004277"
     CDS             2674..3687
                     /gene="rssB"
                     /locus_tag="ACHOS5_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP9779000.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3889..4797
                     /gene="galU"
                     /locus_tag="ACHOS5_004278"
     CDS             3889..4797
                     /gene="galU"
                     /locus_tag="ACHOS5_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9779001.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(4941..5354)
                     /gene="hns"
                     /locus_tag="ACHOS5_004279"
     CDS             complement(4941..5354)
                     /gene="hns"
                     /locus_tag="ACHOS5_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP9779002.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5958..6575
                     /gene="tdk"
                     /locus_tag="ACHOS5_004280"
     CDS             5958..6575
                     /gene="tdk"
                     /locus_tag="ACHOS5_004280"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9779003.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNKGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(6878..9553)
                     /gene="adhE"
                     /locus_tag="ACHOS5_004281"
                     /gene_synonym="adhC"
     CDS             complement(6878..9553)
                     /gene="adhE"
                     /locus_tag="ACHOS5_004281"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9779004.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            9946..9999
                     /gene="ychE"
                     /locus_tag="ACHOS5_004282"
     CDS             9946..9999
                     /gene="ychE"
                     /locus_tag="ACHOS5_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP9779005.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            10030..10677
                     /gene="ychE"
                     /locus_tag="ACHOS5_004283"
     CDS             10030..10677
                     /gene="ychE"
                     /locus_tag="ACHOS5_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP9779006.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(10835..>11025)
                     /locus_tag="ACHOS5_004284"
     CDS             complement(10835..>11025)
                     /locus_tag="ACHOS5_004284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779007.1"
                     /translation="IQSNRGWLSMSLFSDYSSSSEMHNNLTIDYYLALSSTKGSGITN
                     IISIILQQAQDYDVAKIT"
BASE COUNT         2845 a         2455 c         2688 g         3037 t
ORIGIN      
        1 gtcgatgacg gcagatttac agtctgctcc ctttggccgc tcgggaaccc caccacgggg
       61 taatgctttt tactggcctg ctcccttatc gggaagcggg gcgcatcata tcaaatgacg
      121 cgccgctgta aagtgttacg ttgagaaaaa tgaactggtt gcgtaatttt catccgtaac
      181 ggattaaagc taaccagtta tttttgctgg cgattaaaga ataatcgtcc gattaccgta
      241 aacaaagacg cgctgcgcca gtactttgta tagcgcacga cttaagacgt ttttctcgac
      301 gtcacgacct gcgcgcatca tatcttcagc tgtgtaggta tgatcgacat gaataacgtc
      361 ctgcatgatg attgggcctt cgtccagatt gtcattcaca tagtgagcgg ttgcgccaat
      421 aatcttcacg ccacgctcat aggcctggtg ataaggacgt gcgccgataa acgctggcag
      481 gaatgaatgg tgaatattga tgatcttatt cgggaagcgt gccacaaatt ccggcgttaa
      541 tacccgcata tacttcgcca gcaccacata gtcaggttga taagcatcaa tggcatccgc
      601 catcttttga tcgtgctcgt tgcggcttaa cccttcatgg cttaccagct caaacggaat
      661 atcaaaccgc tcaaccagag aacgtaaagt atcgtggtta ccaataactg ccgcgatttc
      721 gacatccagg ccaccgtaat tggctttcat caacaaatcg ccaaggcaat gcgcttcttt
      781 agtgaccaga attactatcc ggcgacgacc ggcaggattc agctcacgca cggagccttc
      841 tggcaatgcg ctatcgagat ccgccagcag ggtggaatca ttaaaaatcc cttccagttc
      901 cgtgcgcata aaaaagcgcc cggtacgatg atctacaaat tcattgttct gcacgatatt
      961 taactcgtgc ttgtagcaaa tattggtaat acgtgcgatc agaccttttt ggtccggaca
     1021 aatagtacgc agaactttac gttggagtga atgcattgct ggaaaaacct tgttgagagt
     1081 gtttgctaaa ccgtaccgtc aaccattatt ggccgcagca ctttttaaat tttttacctg
     1141 aaccacaagg gcagggatcg ttgcggccaa actgcggacg tgtatcgtca atatagtacc
     1201 attgaccgtt ttctttcaaa aatcgcgaac gttcaattat tgcacctgtt ttgccaccct
     1261 cggtaaatcg agcaacaaaa ctgacaaatc cgatattgcc gccgtcctgc cagcaatgtt
     1321 caaatacggt caatcccaac cattcagtat gcgcaaatcc ggcaatcaat tcgacacgta
     1381 acgccgttgc tccacaagat ggatgccagg tctttattaa ataatctgcg tcttgcatca
     1441 caaaagcgca gtaacgcgaa cgcatgagat gttctggatc aggtgcaacc ttttcaccag
     1501 acacataagg gtggcaacat aggctatact cgacagcact accacaggga caaagctgag
     1561 acacaaataa tctccctgga aacaataacg gcgtattaac cgcctgagta gcactatgtt
     1621 aaccgagcag tagcgatgtg gctacgattg cattccaggg gaatcttgcg ggaataatga
     1681 gaaagataaa aatagggctg gcgctgggat ctggcgcggc aagaggttgg tcgcatattg
     1741 gcgtaattaa tgcgctaaaa aaagtgggta ttgaaattga tatcgttgca ggatgttcaa
     1801 ttggttcgct ggtgggcgct gcctatgcat gcgatcgatt atctgcgctg gaagattggg
     1861 tgacctcttt cagttattgg gatgttttac gcctgatgga tctctcctgg cagcgcggtg
     1921 ggttactgcg cggcgagcgt gtcttcaatc aatatcgcga aataatgccg gaaacagaga
     1981 tcgaaaattg ttcccgtcgc ttcgcggctg ttgccaccaa tttaagtact gggcgtgaat
     2041 tatggtttac tgaaggcgat ctccatcttg ctattcgtgc atcatgcagt attccaggac
     2101 taatggcacc cgttgcgcat aacggctact ggctggttga tggcgctgta gttaacccaa
     2161 ttcctatttc cctcacgcgt gcattggggg ctgatattgt gatagcggtc gacctgcagc
     2221 acgatgctca tttgatgcaa caagatttgc tctcctttaa tgtcagtgaa gaaaatagcg
     2281 agaatggtga ttctctgccg tggcatgcgc gtctgaaaga aaggttaggc agcataacga
     2341 cacgtcgggc ggtgacagcg ccaacggcaa cagagattat gaccacttct atccaggtgc
     2401 tggagaaccg ccttaaaagg aaccgcatgg caggtgatcc gcccgatatt ctgattcaac
     2461 ctgtttgccc gcaaatatct acgcttgatt tccatcgcgc gcacgctgcc attgcggccg
     2521 ggcagctggc agtggaaaag aaaatggacg aacttttgcc gttggtacgc accaacattt
     2581 gaccagaatt tttatctaca cttaagttaa ttctgacagg cgtaggtggc aatagcgtgc
     2641 cactattgag taaagccagt caggggagag aacatgacgc agccattggt cggaaaacag
     2701 attctcattg ttgaagatga gcaggtattt cgctcgcttc tggattcgtg gttttcctca
     2761 ttgggagcga caacggtact ggcggctgat ggggtggatg cccttgagtt gctgggaggt
     2821 ttcactccag acctgatgat atgtgatatc gcgatgccac gaatgaacgg gcttaaactg
     2881 ctggaacata tacgcaatag aggtgaccag acaccagttc tggtgatatc tgccactgaa
     2941 aatatggcag atattgccaa agcgttacgt ctgggcgttg aagatgtttt gctgaaaccg
     3001 gttaaagatc tgaatcgctt gcgcgagatg gtttttgcct gtctctatcc cagcatgttt
     3061 aattcccgcg ttgaggaaga ggaaaggctt tttcgcgact gggatgcaat ggttgataac
     3121 cctgctgcag cggcgaaatt attacaggaa ctacaaccgc cagttcagca ggtgatttcc
     3181 cattgccggg ttaattatcg tcaattagtt gccgcggaca aacccggcct ggtgcttgat
     3241 attgccgcac tttcggaaaa cgatctggca ttttattgcc ttgatgtcac cagagctggg
     3301 cataatggcg tacttgctgc cttgttatta cgcgcattgt ttaacggatt attacaggaa
     3361 cagcttgcac atcaaaatca acggttgcca gagttgggcg cgttattgaa gcaggtaaac
     3421 catttactcc gtcaggccaa tctgccgggg cagtttccgc tattagttgg ctattatcat
     3481 cgcgaactga aaaatctcat tctggtttct gcgggtctga atgcgacgtt aaataccggc
     3541 gaacatcagg tgcaaatcag taatggtgtt ccgttaggca ctttaggtaa cgcttatttg
     3601 aatcaattga gccagcgatg cgatgcctgg caatgccaaa tatggggaac cggtggtcgg
     3661 ctgcgcttga tgttgtctgc agaatgagca aacgataaca cgggctaaat ttgcattacc
     3721 tgctaatgtc ggctggtggt actatcgtcg ctattcgtat aagtaattgt cttaattatg
     3781 ctaactcgcc tccttttcag aacttagccc cttcggggtg ctgatatact gggatgcgat
     3841 acagaaatat gaacacgttc aaaacacgaa cagtccagga gaatttaaat ggctgccatt
     3901 aatacgaaag tcaaaaaagc cgttatcccc gtagcgggat taggaaccag gatgttgccg
     3961 gcgacgaaag ccatcccgaa agagatgctg ccacttgtcg ataagccatt aattcaatac
     4021 gtcgtgaatg aatgtattgc ggctggcatt actgaaattg tgctggttac tcactcatct
     4081 aaaaactcta ttgaaaacca ctttgatacc agttttgaac tggaagcaat gctggaaaaa
     4141 cgtgtaaaac gtcaactgct tgatgaagtg cagtctattt gtccgccgca cgtgactatt
     4201 atgcaagttc gtcaggggct ggcgaaaggc ctgggacacg cggtattgtg tgctcacccg
     4261 gtagtgggtg atgaaccggt agctgttatt ttgcctgacg ttattctgga tgaatatgaa
     4321 tccgatttat cacaggataa cctggcagag atgatccgcc gctttgatga aacgggtcat
     4381 agccagatca tggttgaacc ggttgctgat gtgaccgcat atggcgttgt ggattgcaaa
     4441 ggcgttgaat tagcgccggg tgaaagcgta ccgatggttg gcgtggttga aaaaccaaaa
     4501 gcggatgttg cgccgtctaa tctcgctatt gtgggtcgtt acgtacttag cgcggatatt
     4561 tggccgttgc tggcaaaaac ccctcctgga gctggtgatg aaattcagct caccgacgca
     4621 attgatatgc tgatcgaaaa agaaacggtt gaagcctatc atatgaaagg gaagagccat
     4681 gactgcggta ataaattagg ttacatgcag gccttcgttg aatacggtat tcgtcataac
     4741 acccttggca cggaatttaa agcctggctt gaagaagaga tgggcattaa gaagtaacat
     4801 ccgtatcggt gttttccacg aaacggcgtt gagcaatcga cgccgttttt ttatagctta
     4861 ttcttattat attgtcttaa accggacaat aaaaaatccc gccgatggcg ggatttttag
     4921 catgtgcaat ctacaaaaga ttattgcttg atcaggaaat cgtcgaggga tttaccttgc
     4981 tcatccattg cttttttgat tacagctgga gtacggccct ggccagtcca ggttttagtt
     5041 tcgccgtttt cgtcaacgta gctatatttt gccggacgct gagcacgctt agctttggtg
     5101 ccagatttaa cggcagcaag gctattcagc agttcgttcg ggtcaatacc gtcagcgatc
     5161 agcatttcgc gatattgctg cagtttacga gtgcgctctt caacttcagc agcagccgcg
     5221 ctttcttctt cgcgacgttc gttaacaaca acttctaatt tttccagcat ttcttccagc
     5281 gtttcaagtg tacattctct tgcctgcgca cgaagagtac ggatgttgtt cagaatttta
     5341 agtgcttcgc tcattgtagt aatctcaaac ttatattggg gtggtttgtt gaggtaataa
     5401 tagagcctta aattcagttg tgcaatagcc aggaatgtaa ggaattcaaa attgttcttt
     5461 attttgtgcc gccaataaat atcttttcat aaaattagcc agaaaagacg cggcatatag
     5521 ccctatttgc accgatgatt tcgcagcacg tgaggttaaa acttcctgat tcatgtcaca
     5581 ttttatgggg agataatcgt aggctgacga cctttcagtc ttctgtatta gtcgtgttta
     5641 cgagaattcc ctattaagcg aatgatgaaa agtagaacag tcgcaataag agcagggact
     5701 tagtattgca ctatctcctg gaggtcaaca gagggctatt acatgcgcaa caggttaaag
     5761 attgtgaata gttaccggca gtcatttacc cgcttataac aagcgaggca gttgtaatga
     5821 tagctcagaa ggattatgca aggcttcgta agggagaacg catataccac ttctgtgcat
     5881 actgttgagc tgaaaaactg acgaattatg ataaactcca gccaacttta tttcatatca
     5941 ttgagggcct gtggctgatg gcacagctat atttctacta ttccgcaatg aatgcgggta
     6001 agtctacagc attgttgcaa tcttcataca attaccagga acgcggcatg cgcactgtcg
     6061 tatatacggc agaaattgat gatcgctttg gtgccgggaa agtcagttcg cgtataggtt
     6121 tgtcatcgcc tgcaaaatta tttaaccaaa attcatcatt atttgatgag attcgtgcgg
     6181 aacatgaaca gcaggcaatt cattgcgtac tggttgatga atgccagttt ttaaccagac
     6241 aacaagtata tgaattatcg gaggttgtcg atcaactcga tatacccgta ctttgttatg
     6301 gtttacgtac cgattttcga ggtgaattat ttattggcag tcaatactta ctggcatggt
     6361 ccgacaaact ggttgaatta aaaaccatct gtttttgtgg ccgtaaagca agcatggtgc
     6421 tgcgtcttga tcaagcaggc agaccttata acaaaggtga gcaggtggtt attggtggta
     6481 atgaacgcta cgtttctgta tgccgtaaac actataaaga ggcgttagaa gtcggctcat
     6541 taacggctat tcaggaaagg catcgccacg attaataaga atatctttac tgacaggttg
     6601 tgcagggcgc ttttatcctg tcagttcgtt ttacgcactt cttccgggct atacaccctt
     6661 ctcggcagtt ttttaacgcc gctatacgcc tcacagagcg cttttacagt gacgttgact
     6721 tgtgacctgt aaagtacaat atcgctgtgt gtaggcatta tacatcgccg taaatatgat
     6781 gaaggcaaat gctgtcggtt tatgggaaag ttgctttggg taaaacaaaa aaacggcccc
     6841 agaaggggcc gtttatgttg ccagacagcg ctactgatta agcggatttt ttcgcttttt
     6901 tctcagcttt agccggagcg gcttctttct tcgctgcagt ttcgccttct acataatcac
     6961 gaccgtagta ggtatccagc agaatctgtt tcagctcgga gatcagcgga taacgtgggt
     7021 tagcgcccgt gcactggtca tcgaatgcat cttcagacag tttatccaca ttcgcaagga
     7081 agtctgcttc ctgaacgcca gcttcacgga tagatttcgg aatacccagt tcagctttca
     7141 gcgtttccag ccatgccagc agtttctcga tcttagctgc agtacggtcg cccggtgcgc
     7201 tcagacccag gtggtcagca atttcagcat aacgacggcg agcctgcgga cggtcatact
     7261 ggctgaatgc agtctgcttg gtcgggttgt cattcgcgtt gtagcgaata acgttacaaa
     7321 tcagcagggc gtttgccaga ccgtgcggaa tatggaactg ggaacccagt ttgtgcgcca
     7381 ttgagtgaca tacacccagg aaggcgttcg caaacgcgat acccgcgata gtcgctgcac
     7441 tgtgaacacg ttcacgcgct accggatttt tagacccttc gtggtaggac gctggcagat
     7501 attctttcag cagtttcagt gcctgcagag cctgaccatc agagaactca gatgccagta
     7561 cagaaacata agcttccatg gcgtgagtta ctgcgtccag accaccgaaa gcacacaggg
     7621 acttcggcat gtccataacc aggttggcgt cgacaatcgc catatccggg gtcagtgcat
     7681 agtctgccag cggatatttc tgaccagtag tgtcgtcagt tacaaccgca aacggagtga
     7741 cttcagaacc tgtaccagaa gtggtggtga cagcgatcat tttcgctttc acacccattt
     7801 tcgggaactt gtagatacgt ttacggatat ccataaagcg cagcgccagt tcttcgaagt
     7861 gagtttccgg atgttcgtac ataacccaca tgatcttcgc agcgtccatc ggggaaccac
     7921 cacccagcgc gataatcacg tctggtttga aggagtttgc cagttctgca cctttacgaa
     7981 cgatgctcag ggtcgggtca gcttctactt cgaagaagac ttcagtttca acgcctgctg
     8041 ctttcaatac ggaagtgatc tgatcagcat aaccattgtt gaacaggaag cggtcagtca
     8101 cgatgagcgc acgtttgtgg ccatcagtaa tcacttcatc cagcgcgatt ggcagggagc
     8161 cacggcggaa gtagatagat ttcggaagtt tgtgccacaa catgttttca gctcgcttag
     8221 caacggtttt cttgttgatc aggtgtttcg gaccaacgtt ttcagagatg gagttaccac
     8281 cccaggaacc acaacccaga gtcagggaag gtgcgagttt gaagttatac aggtcaccga
     8341 taccaccctg agaagccggg gtgttaatca ggatacgagc cgttttcatt ttctgaccga
     8401 agtaagaaac gcgagccggt tggttatcct ggtcagtgta caggcaagag gtatgaccga
     8461 taccgcccat agcaaccagt ttctctgctt tttctaccgc gtcttcgaaa tctttagcac
     8521 ggtacattgc cagagtcggg gacagttttt catgtgcgaa cggttcgctt tcatcaacaa
     8581 cggtcacttc accgatcaga atcttggtgt tttctggtac agagaagcct gccagttcag
     8641 caattttata ggctggctga ccaacgatag ccgcgttcag cgcaccgttt ttcaggataa
     8701 cgtcctgaac agctttcagc tctttaccct gcaacagata gccgccgtgg gttgcgaaac
     8761 gttcacgtac tgcgtcataa acagagtcaa caacaacaac agactgttca gaagcacaga
     8821 ttacaccgtt gtcgaaggtt ttggacatca gtacagatgc aactgcacgt ttgatatcag
     8881 cagtttcatc gataacaacc ggagtgttgc ccgcgcctac gccgatagct ggtttaccgg
     8941 agctgtatgc ggctttaacc atgcccggac caccagtcgc gaggatcagg ttgatgtctg
     9001 ggtggtgcat cagtgcgtta gacagctcaa cagaaggttg atcgatccag ccgatcagat
     9061 ctttcggagc accggcagcg atagcagcct gcagaacgat atcagccgct ttgttggttg
     9121 catcttttgc acgcgggtgc ggggagaaga taatggcgtt acgggtcttc aggctgatca
     9181 gcgatttgaa gatagcagtt gaagtcgggt tagtggtcgg aacgataccg caaataatac
     9241 cgatgggttc agcgatagtg atggtaccaa aagtgtcgtc ttcagacaga acaccacagg
     9301 ttttttcatc tttataagcg ttgtagatat attcagaagc aaagtggttt ttgatcactt
     9361 tatcttcgac gatacccatg ccggattcgg caacggccat tttcgcgagt gggattcgag
     9421 catctgcagc agccagagcg gcggcgcgga agattttgtc tacttgctct tgagtgaaac
     9481 tggcatattc acgctgggct ttttttacac gctctacgag tgcgttaagt tcagcgacat
     9541 tagtaacagc cataatgctc tcctgataat gttaaacttt tttagtaaat catctgctcg
     9601 aatacgagag tatagtcagt gcggtgatga tttgcttaac cgatggaaat caaaagctta
     9661 ctcgcgctca cactcactgt gatttactaa aagagtttaa acattagagt tattatctct
     9721 aatgcgtcac ttccaggtgg cgtaagcaag attactcact tctgggtact gattacgtga
     9781 tccaaatcaa aattttgcaa agctgacacc tttcagcatg gcttttcgcc attatagcta
     9841 acagttaata aattgtagta tgatttgttg gctacattag catgttttgc acaactagat
     9901 aacaataacg aatgatagca attttaagta gttaggaggt gaaaaatgct gtcaaaaggc
     9961 gtattgtcag cgcgtctttt caaccttatt tatggctaac attatccggc ttttgcttcg
    10021 gagctaaccg tgattcagac cctttttgat tttcccgttt acttcaaatt tttcatcggg
    10081 ttatttgcgc tggtcaaccc ggtagggatt attcccgtct ttatcagcat gaccagttat
    10141 cagacagcgg cagcgcgaaa caaaactaac cttacagcca acctgtctgt ggccattatc
    10201 ttgtggatct cgctttttct cggcgacacg attctacaac tttttggtat atcaattgat
    10261 tcattccgta tcgccggtgg catcctggtg gtgacaatag cgatgtcgat gatcagcggc
    10321 aagcttggtg aggataaaca gaacaagcaa gaaaaatcag aaaccgcggt acgtgaaagc
    10381 attggtgtgg tgccattggc gttgccgttg atggcggggc caggggcgat cagttctacc
    10441 atcgtctggg gtacgcgtta tcacagcatt agctatctgt ttggtttctt tgtggctatt
    10501 gcattgttcg ctttatgttg ctggggattg ttccgcatgg caccgtggct ggtacgagtt
    10561 ttacgccaga ccggcatcaa cgtgattacg cgtattatgg ggctattgct gatggcgttg
    10621 gggattgaat ttatcgttac tggtattaag gggattttcc ccggcctgct taattaattc
    10681 ctttcaaatg aaacggagct gccatgctcc gtttacttcg tcattatttt tactttgttc
    10741 ctgcgcagtt atcaaaagca aaaggaatag gtaaaaatat tcttctcaaa ttacagttag
    10801 ttatagggat ttccttaacg gcttctcctc accatcatgt tattttcgcc acatcataat
    10861 cctgggcttg ctgaagaata attgaaatga tattagtaat tccactgcct ttggtagagg
    10921 aaagtgctaa ataataatca attgttaaat tattgtgcat ttcactactg gaactgtaat
    10981 cagaaaagag agacatgctt aaccaacctc tatttgattg aattg
//
LOCUS       DAZKHK010000149        11000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKHK010000149 DAZKHK010000000
VERSION     DAZKHK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.150"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..552
                     /locus_tag="ACHOS5_004285"
     CDS             <1..552
                     /locus_tag="ACHOS5_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779008.1"
                     /translation="SDWQKYKKIIDKDVKTLHEKLFNKYHLNTYSFYGKAKEHKIPEA
                     HRTQETAIWRGALASGEMGATPPEPKMNDDRLYLSEVSADRTVKDELSPEEQAWKLKG
                     DNGVMYGWVGQRFTLRDACENGDGTVPLRAGQIVHANIQERLAVPALHEAAYRHDVSQ
                     AFALRSIIKIAQQVNKDEKMAFI"
     gene            530..1648
                     /locus_tag="ACHOS5_004286"
     CDS             530..1648
                     /locus_tag="ACHOS5_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity protein Tli4 family protein"
                     /protein_id="HFP9779009.1"
                     /translation="MKKWPLFNGPHRLRNGLLLAALIAITGWLALKPGSYHYSLSDRE
                     KEMVTSLLQHPETRYFGFYSVALPAEFTPTGMVMFIQGSEMTPVETKLQYYPPFQQFL
                     LRYEEELRNKPVTDPRDAPYLKQVYQQEPPLKGAIFERNEESFTPDMARVLDAWKWAE
                     GVTFSVKMKARDERAARYDGYWRGKSKEVSDTFRYNVPQKKAQLLAILSGLQPRKDNQ
                     RPSENKLAIQYGQVDASLLGQYEASVSYQNSKEITITLKTDNHSYIGTPLLDKDARVM
                     ETERGETLYKGKRKVNGLEISEWWNKQHDNYSSEPAINYNFELVIHEDKKGGNKLLEL
                     SMDYSIDLLHADNALTEHELVALWESITGTIKYHPTQW"
     gene            1745..2848
                     /locus_tag="ACHOS5_004287"
     CDS             1745..2848
                     /locus_tag="ACHOS5_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity protein Tli4 family protein"
                     /protein_id="HFP9779010.1"
                     /translation="MKKWPLLNDPHRLRNGLLLATLILIAGWLVLKPGSYHYSLSDRE
                     KEMVTSLLQHPEMRYFGFYSVALPAEFTPTGMVMFIQGSEMTPVETKLQYYPPFQQFL
                     LRYEKELRNKPVADPQDAPYLKGVYPLTSPMSGLIFERVESGDTPDFARILDAWKWAE
                     GVTFSVKMNVTDRRPARYDVDRKGSPGIYSYDVPQKKAQLLAILSGLQPRRDNQRPSG
                     NKLAIQYGQVDASLLGEYEVSVSYKNSNNIEISLQTDTDSYITSLLDENAQVMETADG
                     ITLYKGQKKINGLEISEWWVRKQIMLSSEPSRDYEFSLAIHEDKKNNKQLLKLVMNYS
                     IDLLHADNALTEHELVALWENITGTIKYHPTQW"
     gene            3004..3264
                     /locus_tag="ACHOS5_004288"
     CDS             3004..3264
                     /locus_tag="ACHOS5_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP9779011.1"
                     /translation="MPGIVCLGDATTHGGKVITATSTLFINGIQVALVGDLVSCPKHG
                     VNPIQEGDPTATEKGRNVVVNHCQCACGCRVISSQPENSIES"
     gene            3266..4402
                     /locus_tag="ACHOS5_004289"
     CDS             3266..4402
                     /locus_tag="ACHOS5_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779012.1"
                     /translation="MGWPVPDIPERKMLPEPVYRRWIILLISMLTVGTLFILSVWNSA
                     TYWDIFIYGVLPMLFLWLCLFGIALNKYEQSVAACISWESERQQVKQLWQHWSQKQLA
                     IVGNVLFTPEEKGMSVLLGPQEEIPAYPKKARPLFSASRYSLSSIFHDIHQQLTQQFP
                     DYRHYLHTIYVLQPEKWRGETVRQAIFHQWDLVPERTNTLNQIQSLYDERFDGLILVV
                     CLQNWPENKPEDTSELVSAQLISSSSFVRQHQIPVIAGLGRVMPLEPEELEHNLDVLF
                     EYNQLDNKQLQHVWVSGLDEGTIENLMQYAEQHQWSLPKKRPLHMIDHSFGPTGEFIF
                     PVSLAMLSEAAKETEQNHLIIYQSAQYAQKKSLCLITRKLYLRT"
     gene            4412..7765
                     /locus_tag="ACHOS5_004290"
     CDS             4412..7765
                     /locus_tag="ACHOS5_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP9779013.1"
                     /translation="MHTQRYGFWGYLILIIGLAGICALLIIINQDVLIKSGVSARLIW
                     GIWAGLFSLFLLGVVCSPFWWIRKQRKAQFIYRPKNSAENDQPVSSPSEDILTDLSSN
                     IRLRYGPFWRRKVRLLLVTGEPEEAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVT
                     LLAALKKLRRSRPLDGIIWPLTEEQSRQTAQLDKGWRELVNGSKRLGFQAPLYLWQVC
                     DNGDYQTGRPLQSVGCLLPERCTPEQLAAMLEAQTLPLTEQGMSQLLADNRHDFLLRL
                     AHTLAERGIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAG
                     VTGDNARGRTVGFPWLRTALMSAVCVLVIWGAGMTTSFFANRALVQETGIQTARALDT
                     RLPLAEQLVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRL
                     VRDVAVDHLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTL
                     MADGLRYENIPEGVRQSVLPSLLTFWTANLPEHPQWKTSPPPELTGAVRKILLRQIGV
                     RNAENTLYQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVRE
                     AIEQVVTARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRW
                     KKEDSLSGILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNG
                     KEKTPQQSREGNEPVGPLDKTFAPLLRLLGDKAGGGGGDTQLSLQTYLTRVTRVRLKL
                     QQVTNAPDPQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWNGFGQALFVRPV
                     EQSWRQVLTPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGR
                     INQFIAANLSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGI
                     HFELRAKPARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAME
                     RILADYRGSWSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPMALLE
                     LKNFRLPGQVFLTGKSMKDAEEYGEDADE"
     gene            7758..9368
                     /gene="tssA"
                     /locus_tag="ACHOS5_004291"
     CDS             7758..9368
                     /gene="tssA"
                     /locus_tag="ACHOS5_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9779014.1"
                     /translation="MSELLQKLTRSCFADRDALDVARTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLSGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKDDFANI
                     VAALNQLTVSFAAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGVP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALAMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVAIDPARAAVLCDTQHKD"
     gene            9374..10804
                     /locus_tag="ACHOS5_004292"
     CDS             9374..10804
                     /locus_tag="ACHOS5_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP9779015.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGGNEPEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEALSLEVLDGNEVIYSERQTGKYCDKGEHAWQWDGYSNQGILDTTKIKS
                     KSLLVRLIALCGDMMIKVDYPLKTSANEEDWIDVKVDKNQKIVDVSWRVEFEDDGVSH
                     KDDRIAPVGFEKLKQLAKDGMEYYWARNGMRNPGIGNNITTPHGKYNVNISVSINIDP
                     AMDSFDLIEEQDLESVRSSAFMGRMNIYFNRGYYEYGRGYKKGADPVFKAGLEFKLDV
                     AHETGHMILKSYGGNTYSWEHKGTSNLVPPQYALPHSVYPGTGEIDLMKYYDGHIYTS
                     DLYARSVAVENDVCAMIWLSRVKFHD"
BASE COUNT         2934 a         2560 c         2979 g         2527 t
ORIGIN      
        1 tctgactggc agaagtataa aaaaatcatt gataaagacg tcaaaacgct gcatgaaaaa
       61 ttatttaata aataccatct aaatacctac tcattttatg gaaaagcaaa agagcataaa
      121 attccagaag cgcaccggac tcaggaaaca gcaatctggc gcggcgcact ggcaagtggt
      181 gaaatggggg cgacgcctcc agagccaaaa atgaatgacg atcgccttta tttaagtgag
      241 gtcagtgccg acagaacggt gaaagatgag ctttcgccgg aagagcaggc atggaaatta
      301 aagggcgata acggtgtgat gtacggttgg gtaggacagc gttttacact acgggatgcc
      361 tgtgaaaatg gcgacggtac ggtgccgctg cgtgccgggc aaatcgttca cgcgaatatt
      421 caggagcgtc tggcagtacc ggctcttcac gaagcggctt accgtcatga cgttagtcag
      481 gcttttgcct tacgctctat tataaaaatc gctcagcagg taaataaaga tgaaaaaatg
      541 gcctttattt aatggcccgc accgtctgag aaacgggtta ctgctggctg cgttgatagc
      601 aataaccgga tggctggcac tcaaacccgg cagctatcat tattcattaa gtgacaggga
      661 gaaagagatg gtcacctcgt tattgcaaca ccccgaaacg cggtattttg gcttctattc
      721 cgtggcgctt ccggctgaat ttacgccaac gggaatggtg atgtttatac agggctcaga
      781 gatgaccccg gtggaaacaa agctgcaata ttaccctccg tttcagcagt ttctgctcag
      841 gtatgaggaa gaactcagaa ataagcctgt aacagatcct cgggatgcac cttatcttaa
      901 gcaggtttat caacaggagc caccactaaa aggcgctatt tttgaacgta atgaagagtc
      961 ttttactcca gatatggcaa gagtattaga tgcgtggaaa tgggctgagg gggtcacatt
     1021 ttcagttaaa atgaaagccc gagacgaacg ggcagcccgc tacgatggtt actggcgagg
     1081 taagagtaaa gaagtttctg atacctttcg atataatgtc ccacagaaaa aagcacaatt
     1141 actggctatc ctgtcaggat tacagccccg caaagacaac cagcgcccgt cagaaaataa
     1201 gttagccatt cagtacggac aggttgatgc ttcactgctc gggcagtatg aagcctccgt
     1261 aagttatcaa aacagcaaag aaatcactat aacattaaag acggataacc acagttatat
     1321 tggtacaccg ttactggata aagatgcccg tgtaatggaa acagagcgtg gtgaaacgtt
     1381 atataaaggg aaacgcaagg tcaacggtct tgagatttca gagtggtgga ataaacagca
     1441 tgataattat agttcagaac ccgctataaa ctataatttc gaactggtta tacatgaaga
     1501 taagaaagga ggaaataagt tacttgagtt gtcgatggat tacagtattg acctcttaca
     1561 cgctgataac gcattaaccg aacatgagct tgtggcactg tgggaaagta taacagggac
     1621 aattaaatat catcctactc aatggtaaag gaagataatt agatattgcc tgattaaaga
     1681 taacgcctcc gtcaaacttt ggcattacgc tctattataa agatcgctca gcaggtaaat
     1741 aaagatgaaa aaatggcctt tacttaatga cccgcaccgc ctgagaaacg ggttactgct
     1801 ggcgacgttg atattaatag ccggatggct ggtactcaaa cccggcagct atcattattc
     1861 attaagtgac agggagaaag agatggttac ctcattattg caacaccccg aaatgcggta
     1921 ttttggcttt tattccgtgg cacttccggc tgaatttacg ccaacgggaa tggtgatgtt
     1981 tatacagggt tcagagatga ccccggtgga aacaaagctg caatattacc ctccgtttca
     2041 gcagtttctg ctcaggtatg agaaggaact cagaaataag cctgtagcag atccgcagga
     2101 tgccccctat ctgaaggggg tttatccact gacctcgcca atgagtggac tcatttttga
     2161 acgggtggag tctggagata ctccggactt tgcaagaata ctggacgcgt ggaaatgggc
     2221 agagggtgtc acgttttcag ttaaaatgaa tgttacagac aggcgacctg ctcgttatga
     2281 tgtggatcga aagggatcgc caggtatcta ttcatatgat gtcccgcaga aaaaagcaca
     2341 attgctggct atcctgtcag gattacagcc ccgccgggac aaccagcgcc cgtcaggaaa
     2401 caagctagcc attcagtatg gacaggttga tgcttccctg cttggagagt atgaagtgtc
     2461 cgtgagctat aaaaacagca acaatattga aataagttta cagacggata ctgacagcta
     2521 tattacctca ttattagatg aaaacgccca ggtaatggaa acagcggatg gtataaccct
     2581 gtataaaggt cagaagaaaa ttaatggtct ggagatatct gagtggtggg ttcgtaagca
     2641 aataatgttg agctcagaac catcacgaga ttatgaattc agtctggcaa tccatgagga
     2701 taagaaaaac aacaaacaat tattaaaact tgttatgaat tacagtattg acctcttaca
     2761 cgctgataac gcattaaccg aacatgagct tgtggcgctg tgggaaaata tcacggggac
     2821 gataaaatat catccaacgc aatggtaagg aagataatca agtgtattga aatatccacc
     2881 gcaaaatata aagatagcgg caacgcattt atatcagtgg ctgaaaatgg actttctgct
     2941 ttttaactat attttcactt ttttaagtgt gcttcagttg tgaataaatc agaggtaaaa
     3001 attatgcctg ggatcgtttg tttaggtgat gccacgactc atggtggtaa ggtgattacc
     3061 gcaacttcaa cgctttttat caatggcatt caggtagctc tcgtaggtga tctggtttcc
     3121 tgcccaaagc acggcgtgaa tcccatacaa gaaggtgacc cgacagcgac ggaaaaaggt
     3181 cgtaatgtcg ttgttaatca ttgccaatgt gcatgtggct gtagggttat cagttctcaa
     3241 ccagaaaata gtatcgagtc ataatatggg atggcctgta cccgatattc cagaaagaaa
     3301 aatgctacct gagcctgttt atcgacgctg gattatatta ttaatatcta tgttaacagt
     3361 tggtactctg tttattttat cggtctggaa ttctgcgaca tactgggata tttttattta
     3421 tggcgttctg ccaatgctgt ttctttggct atgtttgttt ggtattgcgc tgaacaaata
     3481 tgaacaatcc gttgcagcct gtataagttg ggagtctgaa agacaacaag ttaaacaact
     3541 ctggcaacac tggagccaaa aacaactggc aatagttggg aatgttcttt ttacaccgga
     3601 agaaaaaggc atgagtgttt tactggggcc acaggaagag atccctgcat atcctaaaaa
     3661 ggcacgaccg ttattctctg catcccgtta ttctctttcg tctatattcc atgatattca
     3721 ccagcaactg acacaacaat ttcctgatta tcgtcattat ctacatacta tctacgtatt
     3781 acagcctgag aaatggcgtg gagaaaccgt gagacaggct attttccatc aatgggactt
     3841 agtacctgaa cggaccaata ctcttaatca aatccagtct ctttatgatg aaagatttga
     3901 cggtctaatt ctggttgttt gtttacaaaa ctggccggag aataaacctg aagatacgag
     3961 tgaactggta tcagcacagc ttatctcctc atcgtcattt gtacggcagc accagatacc
     4021 cgttattgct ggtctggggc gtgtaatgcc attagaaccc gaggagttgg agcataatct
     4081 ggatgtgtta tttgaatata accaattgga taacaaacaa ctacagcatg tctgggtctc
     4141 tggtttagat gagggaacga tagaaaacct tatgcagtat gctgaacaac atcaatggtc
     4201 acttcctaaa aaacggcccc tacacatgat tgatcattcc tttggcccta caggagagtt
     4261 tatttttcct gtctctctgg caatgctgtc agaggctgcc aaagaaactg aacaaaatca
     4321 tttaattatc tatcagtcag cacagtatgc tcagaaaaag agcctttgcc tgattacccg
     4381 gaagctttat ttaaggacat gaggaaaaat aatgcatact cagcgttacg gtttctgggg
     4441 atatttaatt ctgattattg gtctggcagg gatttgcgct ctgttaatta ttattaatca
     4501 ggatgttctg ataaagagcg gagtgtctgc tcgtctgata tggggaatat gggcgggatt
     4561 attttctctt tttttacttg gggttgtgtg ttcgccgttc tggtggatcc ggaagcaaag
     4621 gaaagcgcaa tttatttaca gaccaaaaaa ttcggcagaa aacgaccaac ccgtatcttc
     4681 tccatcagag gatattttaa ccgatctcag ctcaaatatt cggttacgct atggtccgtt
     4741 ctggcggcgt aaagtccgcc tgctgctggt gaccggcgag cctgaagagg cagaggccat
     4801 cgcgccgggg ctgaccgggc aacactggct ggagggcgac cacaccgtgc tgatatatgg
     4861 cggcaggcca tcagcggagc ctgatgtcac actgctggcc gccttaaaaa aactgcgccg
     4921 cagccgtccg ctggacggca tcatctggcc actgacagaa gaacagagcc gtcagacagc
     4981 gcaactcgac aaaggctggc gcgagctggt caacggcagt aagcgactcg gttttcaggc
     5041 cccactctat ttgtggcagg tctgtgacaa cggtgattat cagaccggac gccccctgca
     5101 aagcgtcggc tgcctgctgc cggaacgctg taccccggaa caactggctg caatgctgga
     5161 agcacaaacc ctgccgctga cggaacaggg catgtcgcaa ctgctggcag ataaccgcca
     5221 tgatttcctg ctgcgtctgg cccataccct tgcagagcgg ggtatcgctc actggcaaag
     5281 cgttctgaaa ccgctgctgg caggcggcgc attttcttcc ctgcgcctgc gtggcctgat
     5341 gttcagcccg ccgcttgccg ccgtgccgga ggccgcacct catgcgtggc tgccgtcacc
     5401 ggtctgggcg ggcgtgacgg gtgataacgc gcgcgggcgc acggtgggtt ttccgtggct
     5461 gcgtaccgca ctgatgtccg ctgtctgcgt gctggtgata tggggggccg gaatgacgac
     5521 ctcgttcttc gccaaccgcg ctcttgttca ggaaaccggt atccagacgg cacgtgcgct
     5581 tgatacccgc ctgccgctgg cagaacaact ggtggcgctg cataccctgc agggcgaact
     5641 ggaacgcctg caatatcgta tccgcgaagg tgcgccgtgg tatcagcgtt ttggccttga
     5701 acgtaaccaa caactgctcg ccgccgcttt tcccggctat gcgcaggcgg caaaccggct
     5761 ggtgcgcgac gtggccgttg accatctgca acagcaactt aatgcctttg tcgcgctgcc
     5821 gcccaacagt cctcagcgta ccgccaccgg tgaacaacgc tataagcagc ttaaggcatt
     5881 gctgatgact tcccgcccgg aaaaggccga cgctgccttt ttcagtacca cgctgatggc
     5941 ggacggtctg cgctacgaga atatcccgga aggtgtgcgg cagagcgtgt tgccgtcact
     6001 gctgaccttc tggacggcga acctgccgga acacccgcag tggaaaacat cgccgccacc
     6061 ggaactgacc ggcgcagtgc gtaaaatcct gctgcgccag attggtgtgc gtaatgccga
     6121 aaacaccctc taccagaacg tgctgcaaca ggtgtcccgc aactacgccg atatgacgct
     6181 ggcggacatg accggggata ccctcaccga atctcttttc agtacggaac agacggtgcc
     6241 ggggatgttc acccgtcagg cgtgggaagg acaggtcagg gaagccatcg agcaggtggt
     6301 gacggcgcgg cgcgaggaaa tcgactgggt actcagcgac cgccagcagg atacctctgc
     6361 ggatatctcg ccggatacgc tgcgtaaccg tctcacctca cgctacttta ccgactttgc
     6421 cggaagctgg ctggcgtttc tcaacagcat tcgctggaaa aaggaagact cgctctccgg
     6481 cattctcgac cagctgacac tgatggccga tgcccgtcag tcgccactga ttgcgctgac
     6541 ggacaccctc gcgtggcagg cggcggcagg cagggaaaac cgtggcctgt cagactcgct
     6601 ggcgaaatcg gcacaggaac tgtttaacgg caaggagaaa acgccgcagc aatcccgtga
     6661 aggcaacgag cctgtcgggc cgctggataa aaccttcgcg ccgctactgc ggttgctggg
     6721 cgataaggca ggaggcggtg gtggtgatac gcaactcagc ctgcaaacgt atctgacccg
     6781 cgtcacccgc gtgcgtctca aactgcaaca ggtgaccaac gcccccgacc cgcaggagat
     6841 gacccagcaa ctggcgcaga cggttttaca gggcaaaacc gttgacctca ccgacacccg
     6901 cgactacggg cgattaattg ccgccagtct gggtgaggaa tggaatggct tcggtcaggc
     6961 gctgttcgtc cgcccggtgg agcagtcatg gcggcaggtg ctgacgcctg cggcagacag
     7021 cctgaaccga cagtggcaac gggcgattgt cagtcactgg aatcaggact tcgctggccg
     7081 ctatccattc aaagcctcac agaacgatgc ctctcttccc ttgctggcgc agtacctgcg
     7141 cgatgacgga cgcatcaacc agtttattgc cgccaacctt tccggcgttc tgaaacgtga
     7201 aggccgctac tgggtggctg acgccatgaa cacgcagggg ctgacggtca acccggactt
     7261 tatccgcgcc ctgaaccgcc tgcgcgatgt ggccgatacc gcctttgcca gcggcgatgc
     7321 cggaatacat tttgaactgc gggcaaaacc ggcgcgcgac gtgatgaaga cgcatctggt
     7381 gattgacggg caggaactgg agtatttcaa ccagaaagaa cgctggcagc gttttaactg
     7441 gccggatgaa cagtggcaac ccggcgcatc gctgagctgg accagcacgc aggcaatgga
     7501 acgcatactg gcggattacc ggggaagctg gagccttatt cgcctgctgg aacaggcgca
     7561 ggtgacgccg gtggacagca gcacctttaa ggtggtgtgg aaagcgcagg acggcctgcc
     7621 gctgaattac ctgctgcggg ttgaacaggg taaagggccg atggcgctgc tggaactgaa
     7681 aaacttccgc ctgcctggac aggtattcct gaccgggaag agcatgaagg atgcggaaga
     7741 atatggggaa gacgccgatg agtgaactgc tgcaaaagct gacccgtagc tgttttgctg
     7801 accgggatgc actggatgtg gcgcgaacac aggcggcgct gtggcagaca tggctgctgc
     7861 cggtgacggc agacacgccg gtcggggaag accccggcta ccacgacgat ttcctgcgta
     7921 tccgcgacga gatgaacaaa ctttccggcg cggatacgga cctcatctgc cagcttgccg
     7981 aatccctgct gctgacgcag gcaaaagacg tgcgcatcgc cacttactac atctgggcgc
     8041 gactgcaccg cgacggtgag cgcggactgg cggaaggact ggcgctgctg agcggactgg
     8101 tggaacgctt cggtacgcaa ctgctgccct cgcgaccggc cagcaggaaa atggcgctgg
     8161 agtggctggc gggggaaaag atgctcgaca gccttgcccg ctacccggaa gtggcaaaag
     8221 atgattttgc gaacattgtt gccgctctta atcaactgac ggtttccttc gccgcatggc
     8281 ccgaagacca gcattccccc tccctgatgc cgctgattaa cgcgctggaa tcgcgtctgg
     8341 cacagtcggg cggaatgaac gcggtggtgc cgcagaacag cagcggcgtt ccggcaccgt
     8401 catccccggt ggatgcgccg caggtacaga ccatcacctc cggacgtgac ctgctcgacc
     8461 aggcgaaagt gctggcccgt tatctgaacg aacagccgca gggctggctt tcggcgcacc
     8521 ggctgatgaa aaccctgcgc tgggacacgg tgcatgaact gccgccggat gtggacggca
     8581 aaacccggct ggccccgccg cgcaccgagt cacgcaacca gcttaagcgg ctgtatgccc
     8641 agcagaactg gacggaactg ctggagcagg ccgacctgat gttcagcacc ggcgtcagcc
     8701 atttctggct ggatatccag tggtatctgc atcaggcgct gactaaggcg ggcgtaccgt
     8761 gggacaggtg gacagctgtt atccgtcagg acctggcgtt actgctggaa cgcctgccag
     8821 ggctggaaaa tctggcatgg aacgacggca cgccgttcgc cgatgaggtg acgcgcaact
     8881 ggatagcgca gcaggtcatg atgcgggaag acggcgcatg gcttgcgggg aaagccgctg
     8941 taccgacaga cgacgccaca aatgacgtac tggcgctgga gccggaagca ctggcgatgg
     9001 cagacagtca gggcgtggaa gcggcgctgg gctggataca gacccgtccg ggcatcacca
     9061 cggcacgaca gcgcctgtta ttgcgactgc tgatggcgcg ggtggcggaa cagtacggta
     9121 aaaacgagat ggcgctcctg ctgctggaag aactggatac cgccgcacag ggaataacgc
     9181 tgacgcagtg ggagccagaa ctgctgtttg aagtaaaagc ccggcaactg aaactgctgc
     9241 gtctgcgggc gcaccgctat gcggataaag ccctgctgaa ccgaaaaatg gaaatcctgc
     9301 tgggaacgct ggtggcaatc gatccggcac gggcagcggt actgtgtgat acgcaacata
     9361 aggactgatg gcaatgggaa caggctactt tctggtcagg ggggacaaaa ccacctgcgg
     9421 cgggaagatt attgaaggcg cggacgacca tacgatcatg ggtattccgc aggcacggga
     9481 tatggaccgg gtcacctgcg gcagatatcc gggaatgttt attattgtgg gcggcgttcc
     9541 tgaaacagat attcacggca ggctgatggc ggggacgctg gacagtcaga gcagttgccc
     9601 gtgtaaagca cggtttattg cgtcaatgat ggatgatacc tatgagacgg atgacggggg
     9661 gaacgagccg gaacaacatg cgcagtcggc gaaaaaggat ctgacttctg gttctgattc
     9721 atcctctgat gatgtaaaac tggattacag aattaaatta tcaggtaata agatactgac
     9781 gccactgaat atacctgatt ataaagaaat gattagcggt ggtagtacga agaatacaga
     9841 aaaaatagat tttaccatca caaataaagg cgatgaggct gaagcgcttt ctctggaagt
     9901 gcttgatggt aatgaagtga tttatagtga acggcagacc ggtaaatatt gtgataaggg
     9961 agagcatgcc tggcaatggg atggttacag taatcaaggc atactggata caactaagat
    10021 aaaaagcaaa tcactacttg taagactgat tgctttatgt ggtgatatga tgataaaagt
    10081 tgattatcca ttgaaaactt ctgcgaatga ggaagattgg attgatgtaa aagtagacaa
    10141 gaatcaaaaa atagttgatg tcagttggcg agtagaattt gaagatgatg gtgtgagtca
    10201 taaagatgat cgtattgcac ctgtaggatt tgaaaaatta aagcaacttg ccaaagatgg
    10261 aatggagtat tattgggcac gaaatggaat gaggaatccc ggaattggca ataatattac
    10321 tactccgcac ggtaaatata atgtaaatat ctctgtatca ataaatattg acccagccat
    10381 ggattctttt gatttaattg aggagcaaga tctagaaagc gtcaggtcta gtgcttttat
    10441 gggacgtatg aacatttatt tcaatagagg gtattatgaa tatggccgtg ggtataagaa
    10501 aggcgcagat cctgttttta aggctggatt agaatttaaa cttgatgtag cccatgagac
    10561 tggtcatatg atattaaaaa gttacggtgg gaatacgtat tcatgggagc ataaagggac
    10621 ctctaatctt gtaccacctc aatatgcact gcctcattca gtttatccag ggactggcga
    10681 gatagattta atgaaatatt atgatgggca tatatacacc agtgatctat atgcaaggtc
    10741 ggttgctgtc gaaaacgatg tatgtgcaat gatatggtta tctcgagtga aatttcatga
    10801 ttagattaat attctctttt ttattatgca cactgtgtgc tggatgttta tctcaaccat
    10861 tccattcttg ttccattgtc atggtaaata catgcaaagt atctattagt atttcgacag
    10921 ttaataattc aacacattat gatggtaaag taagagagaa tttccatctt gatgtcggag
    10981 agcgaaagcc aattgtatac
//
LOCUS       DAZKHK010000150        10969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKHK010000150 DAZKHK010000000
VERSION     DAZKHK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.151"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            176..1318
                     /gene="yghO"
                     /locus_tag="ACHOS5_004293"
     CDS             176..1318
                     /gene="yghO"
                     /locus_tag="ACHOS5_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP9779016.1"
                     /translation="MECDLLMIKIEKVINKKDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAITDLNGSLFPFDWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1325..2089)
                     /gene="glcC"
                     /locus_tag="ACHOS5_004294"
     CDS             complement(1325..2089)
                     /gene="glcC"
                     /locus_tag="ACHOS5_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP9779017.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2340..3839
                     /gene="glcD"
                     /locus_tag="ACHOS5_004295"
     CDS             2340..3839
                     /gene="glcD"
                     /locus_tag="ACHOS5_004295"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP9779018.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQMLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3839..4891
                     /gene="glcE"
                     /locus_tag="ACHOS5_004296"
     CDS             3839..4891
                     /gene="glcE"
                     /locus_tag="ACHOS5_004296"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP9779019.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMVGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVADQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4902..6125
                     /gene="glcF"
                     /locus_tag="ACHOS5_004297"
     CDS             4902..6125
                     /gene="glcF"
                     /locus_tag="ACHOS5_004297"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP9779020.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARLVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRIGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6130..6534
                     /locus_tag="ACHOS5_004298"
     CDS             6130..6534
                     /locus_tag="ACHOS5_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP9779021.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6556..8727
                     /gene="glcB"
                     /locus_tag="ACHOS5_004299"
     CDS             6556..8727
                     /gene="glcB"
                     /locus_tag="ACHOS5_004299"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP9779022.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDHVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMAE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9082..10764
                     /gene="glcA"
                     /locus_tag="ACHOS5_004300"
     CDS             9082..10764
                     /gene="glcA"
                     /locus_tag="ACHOS5_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP9779023.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2544 a         2733 c         3078 g         2614 t
ORIGIN      
        1 tcgccttaaa tcgacaagag gcattgtggc gcttctgtgt agtctttgac cttatcgtaa
       61 tcattaataa atactattta ttctttgttt gtattcattg ggttgattat taagatgccg
      121 ttcgttgctg ttgcaacctg tttttcaccg ttaattattt gttatccaca gagatatgga
      181 atgcgatctg ctgatgatta aaattgaaaa agtaattaat aaaaaagacc ttaaggcatt
      241 tatcgctttt ccgtcatcac tttatcccga tgatccaaac tggatacccc ctttattcat
      301 tgaacgtagc gagcatttgt ctgcgaagaa tccggggacg gaccatatca tctggcaggc
      361 gtgggtggca aaaaaagcgg ggcagatagt tgggcgtatt accgcgcaaa tagatacctt
      421 gcaccgtgag cgttacggag aagataccgg tcatttcgga atgattgatg ccattgatga
      481 tccgcaggtt tttgccgcgt tgtttggcgc agcggaagcg tggttaaagt cacaaggtgc
      541 aagtaagatc agcgggcctt tcagcctgaa tatcaatcag gaaagcggat tactgattga
      601 aggttttgac actccgccct gtgcgatgat gccacacggc aaaccgtggt atgccgcgca
      661 tattgaacaa ctgggttatc acaaaggcat tgatttactg gcgtggtgga tgcagcgaac
      721 cgatctcact ttctctccgg cgctaaaaaa actgatggat caggtgcgca aaaaagtgac
      781 cattcgctgc atcaatcgcc agcggtttgc cgaagagatg cagatcctgc gtgagatttt
      841 caattctggc tggcagcaca actggggatt tgtgccgttt accgaacatg aattcgcgac
      901 catgggcgat caacttaaat atctggtgcc ggacgatatg atctacatcg ctgagattga
      961 ttctgcaccc tgcgcgttta ttgttggctt gccgaacatc aacgaggcga ttaccgatct
     1021 gaacggatcg ctctttccct tcgactgggc aaaattgctg tggcgtttga aagtcagcgg
     1081 tgtgcgaact gcgcgagtgc cgctgatggg cgtgcgggac gagtatcagt tcagccgcat
     1141 cggcccggtg attgcgctgt tattgattga agccttacgc gatccgtttg cccgccggaa
     1201 gatcgatgcg ctggaaatgt catggatcct cgaaaccaat acaggcatga ataacatgct
     1261 ggaacgcatt ggggcggagc cgtacaagcg ttaccgattg tatgaaaagc agatttgatg
     1321 gcagtcaccc ctgcttcatc tccagcggca ccgccgagcg aatcaaatgg tggccttcca
     1381 gctcgatatc gtggagattc tttttcacgg tccgcacatg atcgcgtgct gcgcgctggg
     1441 caacgtgcgg cagccgctgc aacaccgcgt tgtagatccg cgcatgctgg cgatcgatct
     1501 gctttttttg ctgtggtcga tggtagagat tatttacgct ggcaaacacc gaattaaaca
     1561 tcaggtcggt caatgattgc agcgtaaaca ccagcacctg attgtgagaa gcctgacaaa
     1621 tggcgagatg gaaagcgtga tccaactgcg catgttcgat cagcgaaatc tctttgttgt
     1681 tctcactggc ggcgagcatt ttttcataac agcgggttat cacaacaaaa tcagcctggg
     1741 ttcccagcgt tgccgccagc cttgccgatt cgccctccag taatgcgcga acgtcgagca
     1801 gatcgtacag cgttcgcggc tgcgtactga acagatggat cagcgggctg gcgtcctgca
     1861 cccgattaag ccgtgcgaca cgagaatcgc gaccctgcgc cgtttcaata atcccgcgcc
     1921 cgcgcagcac ggtcagccct tcacgcagtg cggagcgtga gaagccgagc ttttcacaca
     1981 gtcgacgttc cgagggaagc ggctgaccga ccttcagtac gccgtcgata attaaccgtt
     2041 cgatactttc tgcaaccact tcgcaaatag ggcgacgttc atctttcatt cccggacctc
     2101 gtgcacaggt aggaccaatt tttctgttct taactcgcaa aacacgcaca tcacgtaagt
     2161 gattatgtta catcaattta acattaagtt aaccaaagca aggtcacaga gctggaaaaa
     2221 aaatggtctg accggtagct aaagagatag acgaaaacga aaagcccgct taataactgt
     2281 tcacagaagc agcgcgaaaa aatcagctgc cacacaacac aacaaagcga accctactca
     2341 tgagcatctt gtacgaagag cgtcttgatg gcgctttacc cgatgtcgac cgcacatcgg
     2401 tactgatggc actgcgtgag catgtccctg gactggagat cctgcatacc gatgaggaga
     2461 tcattcctta cgagtgtgac gggttgagcg cgtatcgcac gcgtccatta ctggttgttc
     2521 tgcctaagca gatggaacag gtgacggcga ttctggctgt ctgccaccgc ctgcgtgtgc
     2581 cggtggtgac ccgtggcgca ggcaccgggc tttctggtgg cgcgctgccg ctggaaaaag
     2641 gtgtgttgtt ggtgatggcg cgctttaaag agatcctcga cattaacccc atcggtcgcc
     2701 gcgcgcgcgt gcagccgggc gtgcgtaacc tggcgatctc ccaggccgtt gcgccgcata
     2761 atctctatta cgcgccggat ccgtcctcac aaatcgcctg ttcgattggc ggcaatgtgg
     2821 cggaaaatgc cggtggcgtt cactgcctga aatatggtct aaccgtacat aacctgctga
     2881 aaattgaagt gcaaatgctg gatggcgagg cgctgacgct gggatcggac gcgctggatt
     2941 cgcctggttt tgacctgctg gcgctgttta ccggatcgga aggtatgctc ggcgtgacca
     3001 ccgaagtaac ggtgaagctg ttgccgaagc cgcccgtggc gcgggttctg ttagccagct
     3061 ttgactcggt agaaaaagcc ggacttgcgg ttggtgacat catcgccaat ggcattatcc
     3121 ccggcgggct ggagatgatg gataacctgt cgattcgcgc ggcggaagat tttattcatg
     3181 ccggttatcc cgtcgatgcc gaagcgattt tgttatgcga actggacggc gtggagtctg
     3241 acgtacagga agactgcgag caggttaacg acatcttgtt gaacgcgggt gcgactgacg
     3301 tccgtctggc acaggacgaa gcagagcgag tacgtttctg ggccggtcgc aaaaatgcgt
     3361 tcccggcggt aggacgtatc tccccggatt actactgcat ggatggcacc atcccgcgcc
     3421 gcgccctgcc tggcgtactg gaaggcattg cccgtttatc gcagcaatat gatttacgcg
     3481 ttgccaacgt ctttcatgcc ggagacggca acatgcaccc gttaatcctt ttcgatgcca
     3541 acgaacccgg tgaatttgcc cgcgcggaag aactgggcgg gaagatcctc gaactctgcg
     3601 ttgaagttgg cggcagcatc agtggggaac atggcattgg gcgcgaaaaa atcaatcaaa
     3661 tgtgcgccca gttcaacagc gatgaaatca cgaccttcca tgcggtcaag gcggcgtttg
     3721 atcccgatgg tttgctgaac cctgggaaaa acattcccac gctacaccgc tgtgctgaat
     3781 ttggtgccat gcatgtgcat cacggtcatt tacctttccc tgaactggag cgtttctgat
     3841 gctacgcgag tgtgattaca gccaggcgct gctggagcag gtgaatcagg cgattagcga
     3901 taaaacgccg ctggtgattc agggcagcaa tagcaaagcc tttttaggtc gtcctgtcac
     3961 cgggcaaacg ctggatgtgc gttgtcatcg cggcattgtt aattacgacc cgaccgagct
     4021 ggtgataacc gcgcgtgccg gaacgccgct ggtggcgatt gaagcggcgc tggagagcgc
     4081 cgggcaaatg ctcccctgcg agccgccaca ttatggtgaa gaagccacct ggggcgggat
     4141 ggtcgcctgc gggctggcgg ggccgcgtcg cccgtggagc ggttcggtcc gcgattttgt
     4201 cctcggcacg cgcatcatta ccggcactgg aaaacatctg cgttttggtg gcgaagtgat
     4261 gaaaaacgtt gccggatacg atctctcacg gttaatggtc ggaagctacg gttgtctggg
     4321 tgtgctcact gaaatctcga tgaaagtgtt accgcgacca cgcgccaccc tgagtctgcg
     4381 tcgggaaatc agcctgcaag aagccatgaa tgaaatcgcc cagtggcaac tccagccatt
     4441 acccattagt ggcttatgtt acttcgacaa tgcgttgtgg atccgccttg agggcggcga
     4501 aggatcggta aaagcagcgc gtgaactgct gggtggcgaa gaggttgcgg atcagttctg
     4561 gcagcaattg cgtgaacaac aactgccgtt cttctcgtta ccaggtacct tatggcgcat
     4621 ttcattaccc agcgatgcgc cgatgatgga tttacccggc gagcaactga tcgactgggg
     4681 cggggcgtta cgctggctga aatcgacagc cgatgacaat caaatccatc gcattgcccg
     4741 caacgctggc ggtcatgcga cccgctttag tgccggagat ggtggctttg ccccgctacc
     4801 ggctccttta ttccgctatc accagcagct taaacagcag ctcgaccctt gcggcgtgtt
     4861 taatcccggt cgcatgtacg cggagcttta aggagcaggc tatgcaaacc caattaaccg
     4921 aagagatgcg gcagaacgcg cgggcgctgg aagccgacag catcctgcgc gcctgtgttc
     4981 actgcggatt ttgtaccgca acctgcccaa cctatcagct cctgggcgat gaactggacg
     5041 ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga aggcaacgaa gtcacgctta
     5101 aaacacagga gcatctcgat cgctgcctca cttgccgcaa ttgtgaaacc acctgtcctt
     5161 ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga tattgtcgag cagaaagtga
     5221 aacgcccact gccggagcga atgctgcgcg aaggattgcg ccaggtagtg ccgcgtccgg
     5281 cggtcttccg tgcgctgacg caggtagggc tggtgctgcg accgttttta ccggaacagg
     5341 tcagagcaaa actgcctgct gaaaccgtga aagctaaacc gcgtccacca ctgcgccata
     5401 agcgtcgggt tttaatgctg gaaggctgcg cccagcctac gctttcgccc aacaccaacg
     5461 cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt catgccagct aacgaagcag
     5521 gctgttgtgg cgcggtggac tatcatctta atgcgcaaga gaaagggtta gcgcgggcgc
     5581 gcaataatat tgatgcctgg tggcccgcga ttgaagcagg tgccgaggca attttgcaaa
     5641 ccgccagcgg ttgtggcgcg tttgtcaaag agtatgggca gatgctgaaa aacgatgcgt
     5701 tgtatgccga taaagcgcgt ctggtcagtg aactggcggt cgatttagtc gaacttctgc
     5761 gcgaggaacc gctggaaaaa ctggcaattc gcggcgataa aaaactggcc ttccactgcc
     5821 cgtgtaccct acaacatgcg caaaagctga acggcgaagt ggaaaaagtg ttgcttcgta
     5881 ttggatttac cttaacggac gttcccgaca gccatctgtg ctgcggttca gcgggaacat
     5941 atgcgttaac gcatcccgat ctggcacgcc agctgcggga taacaaaatg aatgcgctgg
     6001 aaagcggcaa accggaaatg atcgtcaccg ccaacattgg ttgccagacg catctggcga
     6061 gcgccggtcg tacctctgtg cgtcactgga ttgaaattgt agaacaagcc cttgaaaagg
     6121 aataacaaaa tgaaaactaa agtcattctt agccagcaaa tggcgagtgc aattattgcc
     6181 gcaggtcagg aagaggcgca gaaaaataac tggtctgttt ccattgctgt tgccgatgac
     6241 ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg cgccgattgc ggcttacatc
     6301 tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg aaactaaggg ctatgaagag
     6361 atggtgaaca acggacgtac cgcgttcgtg actgcgccgt tactgacgtc gctggaaggt
     6421 ggcgtaccgg ttgttgtgga tgggcaaatt attggtgccg tgggcgtttc tggtttaacc
     6481 ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg tgttggcgaa ataagcgaaa
     6541 acgaggagat aaacaatgag tcaaaccata acccagggcc gtttacgcat tgacgccaat
     6601 tttaaacgtt ttgtggatga agaagttttg cctggcgtgg aactggatgc tgccgcgttc
     6661 tggcacaatg ttgatgagat cgttcacgat ctggcgccag agaatcgtca gttgctggca
     6721 gagcgcgatc gcattcaggc ggcgcttgat gagtggcatc gcagcaatcc ggggccggta
     6781 aaagataaag cggcctataa atctttcctg cgtgaactgg gctacctggt gccacagcct
     6841 gaccacgtta cggtggaaac cacgggcatc gacagcgaaa tcaccagtca ggcggggcca
     6901 cagctggtgg ttccggcgat gaacgcccgc tacgcgctga acgcggcgaa cgctcgctgg
     6961 ggctcactgt acgatgcgtt atacggcagc gacatcatcc cgcaggaagg ggcgatggtc
     7021 agcggctacg atccgcagcg tggtgagcag gttatcgcct gggttcggcg tttcctcgat
     7081 gaatcactac cgctggaaaa tggcagctat caggatgtgg tggcgtttaa ggtggtcgat
     7141 aaacaattac gcatccagtt gaaaaatggt aaagaaacca cgttacgtac tccagcacag
     7201 tttgtcggtt accgtggcga tactgctgcg ccgacctgca ttttgctgaa aaataacggc
     7261 ctgcatattg agctacaaat cgatgccaac gggcggattg gcaaagacga ttcggcgcac
     7321 atcaacgatg ttatcgtcga agcggccatc agtaccattc tcgactgcga agattcggtt
     7381 gcggcggttg atgcggaaga taaaatcctg ctgtaccgca acctgctggg cctgatgcag
     7441 gggactctgc aagagaaaat ggagaagaac ggtcggcaaa tcgtgcgtaa actgaatgac
     7501 gatcgtcagt acactgccgc cgatggatct gaaatttctc tgcacggacg ctcgttgctg
     7561 tttatccgca acgtgggtca tttgatgacc attcctgtga tttgggacag cgaaggcaat
     7621 gaaatcccgg aaggcattct tgatggcgtc atgactggcg cgattgccct ctatgattta
     7681 aaagtgcaga aaaactcgcg cactggcagc gtctatattg tgaaaccgaa aatgcacggt
     7741 ccgcaggaag tggcgttcgc caacaaactg ttttcccgcg ttgagacaat gctcggtatg
     7801 gcaccgaata ccctgaaaat gggcattatg gatgaagaac gccggacctc gctgaacttg
     7861 cgtagctgta tcgctcaggc gcgcaaccgc gtggcgttca tcaataccgg tttcctcgac
     7921 cgtaccggcg atgaaatgca ttcggtgatg gaagcgggcc cgatgctgcg taaaaaccag
     7981 atgaaatcga cgccgtggat caaagcctac gaacgtaata acgtgctttc cggtctgttc
     8041 tgtgggctac gtggaaaagc gcaaatcggt aaaggcatgt gggcaatgcc ggacctgatg
     8101 gcagacatgt acagccagaa gggcgaccaa ttgcgtgccg gggcaaacac ggcctgggtt
     8161 ccgtcgccaa ctgccgctac gctccatgcg ctgcactacc accaaaccaa cgtacagagc
     8221 gtgcaagcca acattgccca gaccgagttc aatgctgaat ttgaaccgct gctggacgat
     8281 ctgctgacga ttccggttgc tgaaaacgct aactggtcgg cgcaagagat ccaacaagag
     8341 ctggataaca acgtgcaggg gattctgggg tacgtggtgc gttgggtgga gcaggggatt
     8401 ggttgttcaa aagtgccgga tattcacaac gtggcgttga tggaagaccg cgcaacgctg
     8461 cgtatttcca gccagcatat cgccaactgg ttacgtcacg gtattctgac caaagatcag
     8521 gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc agcaaaacgc tggcgatccg
     8581 gcttatcgtc cgatggcgga gaatttcgct aactcgtgtg cttttaaagc tgcctgcgat
     8641 ttgatcttcc tcggcgtgaa acagccaaac ggctataccg aaccgttatt acacgcctgg
     8701 cgtttacgcg aaaaagaaag tcattaatag cgtcaaaaaa gccccggcaa gttttattgt
     8761 cggggctatg atagtaactg cggtagtaca tttatcccgg aaataaccgg agcgattatc
     8821 agggtttaac aatattaaat acccgtagcg cattactagc gggcctaaaa caacaatatg
     8881 gacatcttaa cgatactctg cgcctgcgtg tgcggaacag aaacccttac ccgatatact
     8941 tgttatgcca gaggtagcaa ttgcgttagc ggcgtactgt tgctgtaaag tataacgcct
     9001 atattttaca gttcagtcct cttttgctta ttacaacctt aagtatatca agcatataaa
     9061 gataataaga gactgaacaa tatggttacc tggacccaaa tgtatatgcc gatgggagga
     9121 ctggggctat ccgctctggt cgccctgatc ccaataatat tcttcttcgt tgcactcgcg
     9181 gtattacgtc tgaaaggaca tgtcgctgga gcaataaccc ttatattatc tattctgatt
     9241 gcgatattcg cctttaaaat gccgattgat atggcatttg ctgctgcggg ctatggcttt
     9301 atttatggtt tatggcctat tgcgtggatt attgtcgcgg cggtattcct gtataaatta
     9361 accgttgcca gcggccagtt cgatattatc cgcagctcgg ttatctccat caccgacgat
     9421 cagcgtttgc aggtgttact gattggtttc tcctttggtg cgctgctgga aggggcggct
     9481 ggctttggcg caccggtggc gattaccggt gctctactgg tgggcctggg cttcaaaccg
     9541 ctatacgcgg cggggctgtg tctgattgcc aacactgcgc cggtggcgtt tggtgcgttg
     9601 ggtgtgccaa ttctggtcgc cggtcaggta acgggaatcg atccgttcga cattggcgca
     9661 atggcgggac gtcagttacc gttcctgtcg gttcttgtgc cgttctggct ggtagcaatg
     9721 atggacggct ggaaaggggt gaaagagacg tggccagcgg cgctggttgc agggggaagc
     9781 ttcgctgtca ctcagttctt tacctctaac tatattggtc cggaactgcc ggatattact
     9841 tcggcgctgg tgagtatcgt ctcactttct ttatttctta aagtctggcg gccaaaaaat
     9901 accgaaacgg caatcagtat gggacaatcc gcaggtgcga tggtggtgaa taaaccgtct
     9961 tctggcggtc ccgtgccttc agaatatcgt ctggggcaaa tcattcgtgc gtggtcaccg
    10021 tttttaatct taacggtgtt ggtcaccatc tggaccatga agccgtttaa agcgttattt
    10081 gctccgggcg gcgcgtttta ttcgctggtg attaatttcc agatccctca tttgcatcaa
    10141 caagtgttga aagcggcccc cattgtcgcc cagccgacgc caatggacgc ggtgtttaaa
    10201 ttcgaccccc tctctgctgg tggcaccgct atttttattg cggcgattat ctcgatcttc
    10261 atcctcggcg tggggatcaa gaaaggtatt ggtgtctttg ccgaaacgct aattagtctg
    10321 aagtggccga tattgtcgat tggcatggtg ctggcatttg ccttcgtcac caactattcc
    10381 ggtatgtcca ccacgttggc gctggtactg gctggtacag gcgtgatgtt cccattcttc
    10441 tcaccgttcc tcggctggct gggcgtattc cttaccggct cggacacttc ttcaaacgcc
    10501 ctgtttggtt cactgcaatc gaccacggcg cagcaaatca acgtctctga caccctgctg
    10561 gtggcagcaa acaccagcgg cggcgtaact ggcaagatga tctccccgca atctatcgcc
    10621 gtggcctgcg ccgcgacggg catggtgggg cgagaatctg aactgttccg ctacaccgtg
    10681 aagcacagtc tgatttttgc cagcgttatc ggcattatca cgctgttgca ggcgtatgtg
    10741 tttaccggga tgttagtttc gtaagtcaat aatgccggat gatcgttaat ctcatccggc
    10801 ttcgtttaca ttattctgtc ttattaatgg aaaaattatt tcccgcttta ttatccatgc
    10861 ggcaataatg ttttgcgttc agataacatg ctaaataatt aagagcgtga gaacatctat
    10921 ttttttcacg caaaacaagt ctgtattaat tatgttcgct tgatgagca
//
LOCUS       DAZKHK010000151        10766 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKHK010000151 DAZKHK010000000
VERSION     DAZKHK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.152"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..193)
                     /locus_tag="ACHOS5_004301"
     CDS             complement(<1..193)
                     /locus_tag="ACHOS5_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779024.1"
                     /translation="MKSIYLKYFLSFVSVGVGAMFICGLLYNDYLGQQPATPEQLTEI
                     LQETPCAAEAFQVTLNYQSE"
     gene            complement(221..439)
                     /locus_tag="ACHOS5_004302"
     CDS             complement(221..439)
                     /locus_tag="ACHOS5_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779025.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGL"
     gene            complement(519..992)
                     /locus_tag="ACHOS5_004303"
     CDS             complement(519..992)
                     /locus_tag="ACHOS5_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779026.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAFCACIAWIPFIIA
                     AFGVDGIYLKILFAVLACCGAVCFFICFFTLLPESLMVLSRQSLVQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQENVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(985..1398)
                     /locus_tag="ACHOS5_004304"
     CDS             complement(985..1398)
                     /locus_tag="ACHOS5_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779027.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(1391..1612)
                     /gene="traR"
                     /locus_tag="ACHOS5_004305"
     CDS             complement(1391..1612)
                     /gene="traR"
                     /locus_tag="ACHOS5_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFP9779028.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     SEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(1747..2262)
                     /gene="traV"
                     /locus_tag="ACHOS5_004306"
     CDS             complement(1747..2262)
                     /gene="traV"
                     /locus_tag="ACHOS5_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFP9779029.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLQRLAEGNFRTMPVQTVTATSPSGSRPAVTALPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVIPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(2259..2510)
                     /gene="trbG"
                     /locus_tag="ACHOS5_004307"
     CDS             complement(2259..2510)
                     /gene="trbG"
                     /locus_tag="ACHOS5_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFP9779030.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            complement(2522..2716)
                     /locus_tag="ACHOS5_004308"
     CDS             complement(2522..2716)
                     /locus_tag="ACHOS5_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFP9779031.1"
                     /translation="MNMRNIHVITALSVPDKRISDDFMHAVLSNCTTGVLPAPEKFGS
                     ESLPDNFTMTAVGVMKNSEI"
     gene            complement(2703..3287)
                     /gene="traP"
                     /locus_tag="ACHOS5_004309"
     CDS             complement(2703..3287)
                     /gene="traP"
                     /locus_tag="ACHOS5_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFP9779032.1"
                     /translation="MANNTSSSQACHTARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEIDFIRQTAPAGQFPVRECLPSLPDLPGAEEKICRYRAADA
                     ADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYLVRPGKMKCIRVVTADEHLKEV
                     YTEDASLPGKIRKCHAYFPDNRTNRNNGDKNEHA"
     gene            complement(3277..4707)
                     /gene="traB"
                     /locus_tag="ACHOS5_004310"
     CDS             complement(3277..4707)
                     /gene="traB"
                     /locus_tag="ACHOS5_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFP9779033.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVLNKQRGDD
                     QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPEGEPQPGNAPVAFPP
                     QGAGAVPPPTAFYPGNGATPPPQVTYQSVPVPNRIQRKTFSYDAGTKQTPSLPYIPSG
                     SFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQVEESNPGSPVPNPPSAESHLNGLNTD
                     QMLKQLGDLNPQQFMAGGQEGGNDGK"
     gene            complement(4707..5435)
                     /gene="traK"
                     /locus_tag="ACHOS5_004311"
     CDS             complement(4707..5435)
                     /gene="traK"
                     /locus_tag="ACHOS5_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFP9779034.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGQLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLVAARMDVYVIRDGEGN"
     gene            complement(5422..5988)
                     /gene="traE"
                     /locus_tag="ACHOS5_004312"
     CDS             complement(5422..5988)
                     /gene="traE"
                     /locus_tag="ACHOS5_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP9779035.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSADASFLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNKMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(6010..6321)
                     /gene="traL"
                     /locus_tag="ACHOS5_004313"
     CDS             complement(6010..6321)
                     /gene="traL"
                     /locus_tag="ACHOS5_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFP9779036.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(6336..6689)
                     /gene="traA"
                     /locus_tag="ACHOS5_004314"
     CDS             complement(6336..6689)
                     /gene="traA"
                     /locus_tag="ACHOS5_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFP9779037.1"
                     /translation="MNTSVSVPGAFAPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            complement(6724..6951)
                     /gene="traY"
                     /locus_tag="ACHOS5_004315"
     CDS             complement(6724..6951)
                     /gene="traY"
                     /locus_tag="ACHOS5_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFP9779038.1"
                     /translation="MIKGALRAAPSRKVLLTLDEATNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVTEERESTFKEL"
     gene            complement(7070..7717)
                     /locus_tag="ACHOS5_004316"
     CDS             complement(7070..7717)
                     /locus_tag="ACHOS5_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFP9779039.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHSIKCQNCDSEIWFSGIDIDTQILFQTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFIWRFFERYVVSSFFIKGKEFCCESDKYRFNKIIPYLLGYSHEYSAQRM
                     DVSIDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINNLHCKET"
     gene            complement(7911..8294)
                     /gene="traM"
                     /locus_tag="ACHOS5_004317"
     CDS             complement(7911..8294)
                     /gene="traM"
                     /locus_tag="ACHOS5_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFP9779040.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            8615..9217
                     /locus_tag="ACHOS5_004318"
     CDS             8615..9217
                     /locus_tag="ACHOS5_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP9779041.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFPDDVTRGEKMRKWLLTICLMFI
                     NVTCKAGDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYSGIKNSKGIQLRASEKPLSKMNSSQKN"
     gene            complement(9513..10334)
                     /locus_tag="ACHOS5_004319"
     CDS             complement(9513..10334)
                     /locus_tag="ACHOS5_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9779042.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREEHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(10445..10741)
                     /locus_tag="ACHOS5_004320"
     CDS             complement(10445..10741)
                     /locus_tag="ACHOS5_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779043.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNNAVKASLAYQSNQDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
BASE COUNT         2901 a         2753 c         2249 g         2863 t
ORIGIN      
        1 gttcagactg ataattaagt gtcacctgaa atgcttctgc tgcacaggga gtttcctgta
       61 aaatctctgt cagttgttct ggtgttgcag gttgttgccc aagataatca ttgtaaagaa
      121 gaccacatat aaacatagcc ccaacaccaa ctgatacaaa agacagaaaa tattttaaat
      181 atatcgactt catttcattt tccttcgaag cagtaagcac tcatagccca ccaccattca
      241 gtgtttccat cacgttattc aaaggttcat tagttttaac ttcaatacac tcccctgtat
      301 ttccaaatcc cctgcctcca tgtgcttcga ttgtccgaag aacctgcacg catacgtccg
      361 tatttggcat gggggtaaca ttccagcttc cgccaccgtt aatatttttt atcagcacaa
      421 gaaaaataaa ggtatgcatt ctttattacc tccgcttgta tattaatcac tgaatatccc
      481 ttcagttaac ttatgaggtc ataaaacact gcagattctc attcggattt attcccttca
      541 gtaaacttcc ggatggtatc ctgttcacgc tgacgggtgg cagactcctg cattgcatcc
      601 acgttctcct gccagagacg ccggatatcc ttttcatccc gaccagtcag ttttttaccg
      661 gacagcagac ggttaagcag ttcctgtctc gcatccggta cgtcttcagt cagacgcata
      721 agctgaacca gactctgacg gctgaggacc atgagtgatt ccggcaggag ggtgaagaag
      781 caaataaaaa agcataccgc accacagcag gcaaggaccg caaacaggat tttcagatat
      841 ataccatcaa cgccgaacgc agcaataata aagggaatcc atgcaataca cgcacaaaac
      901 gccaggatgt tatctattcg gttatgtctg cgatatatct gttgatgttc atccagaata
      961 gaatctattt tttcagcttc tgatttatgc atagtgtttc ctctgcgttt cctgccagag
     1021 acgccggata tcctgttcat cccgaccagt cagtttttta ccggacaaca aacggttaag
     1081 cagttcctgt ctcacatctg gtacgtcttc agtcagacgc ataagccgga gtaattcctg
     1141 acgactgagt accatgcgtg attcctccgg tacgctgtaa taattaaaaa tacgactgaa
     1201 taccccacaa cagcaggcaa cggacaggca caaggatttc agatacaggc cagaaacaca
     1261 gaatgtggca acaccacaaa gaatagctat aactaaaaag cccttaccca gtgccataca
     1321 caaagaaccc tgttttctga atatccgctc atgctcatcc agaataaaat ctattttttc
     1381 attttctgat ttatgcatag tgtttcctct gcctttcctg atacgcctga cattcaacgc
     1441 acaacgtcac accgggaaat attttccgcc gggcttccga aataggattt ccgcatgctt
     1501 cacagagata aacaggaata ccatgaacat ttattttctg gcgtatccgg tttattcctg
     1561 tcatggtcag ttgttctgtt actgaatatg cttcatcggc ttcatcactc acggaatatt
     1621 cctcccgaca tgttctgaca cgctaccgcc catccctttt cacgcacagg gcactcgttt
     1681 tacaggcaga ttttgaatac agaaagcccg ccggcattgc cggctgatat acaacttcag
     1741 ggccagttaa ttaatacgtg gttttcccca cgcagacggt ttaataacaa aaaacacact
     1801 ggatggctga tgataaacat cctgattatc aatataaggc gctatccata atgctgccgt
     1861 ctgctcaccc gttcttagcg gacggggagg tattaccggt gcaactgaac tgaccggaac
     1921 aaccgttttc acttcccggg cagcggtaaa cagcggacgt ggtgccagca gtttctgctc
     1981 cggaagtgcc gtcaccgcag gtctgctgcc ggacggagag gtcgcggtga cggtctgcac
     2041 cggcattgtc cggaagttcc cttcagccag gcgctgcagt gatgccgcaa ccggctttgc
     2101 ttctgaggac tgctccagtt ttttggcctt ctcattggcc tgctccatcg tcatacaggt
     2161 atcggatgtg gtggcatcac actcaaagtc ggagtttgtt ccggcacagc cggaaagtaa
     2221 taacgtcccc agcagcggaa taaataaaga aatctgtttc attgtccctc acctttctca
     2281 tcgcagttca caaaatcatt cttcaaatcc ctgaccgtac aggtttttac aatgtcagga
     2341 ttttcaaaaa ccgtattccg tgatgattta taaacatatg ccacaatgcg tttcccaccc
     2401 gcatcaggtt ctggtgcact gacttcacat aacggaggtg ttattccgga ctcatacagc
     2461 accggataat ttattttttt gacgctgcca tcagaaacca gcttattcat cgaagggttc
     2521 cttatatttc tgaattcttc atcacaccga cagcagtcat agtaaaattg tccggcaatg
     2581 actcagaacc gaatttctcc ggtgcaggaa gtactcctgt ggtgcagtta ctgagaacag
     2641 catgcataaa atcatctgat atgcgtttat ccggtacaga gagggccgta ataacatgaa
     2701 tattacgcat gttcattttt atctccgttg tttctgtttg ttctgttatc agggaaataa
     2761 gcatggcatt tcctgatttt tcccggcagg gaagcgtctt ccgtataaac ttccttcaga
     2821 tgttcatctg cggttaccac ccggatacat ttcatttttc cgggtcgcac cagatatttc
     2881 ccggtgattg ctgccagcga gacatacatc agtgccagtg ttgcccataa tgcggtgacc
     2941 agttgcatca gtgaccggtc agtctccctg acataatctg cagcatcagc agcacggtaa
     3001 cggcagattt tctcttctgc tcctggaaga tctggcagag aaggcaaaca ttctcttaca
     3061 ggaaactgcc cggcaggggc tgtttgtctt atgaagtcaa tttcctgaac cagtaatttc
     3121 cctggtgtgg tgttgtcctt ccataaaaca aataacgcca tcagggccat tgtggccagt
     3181 ggcagaatga cggtgtattt cagacaccag aacagtccac gcagaacccg ggcgacaaca
     3241 taccgggcgg tatggcacgc ctgactggac gacgtattat ttgccatcgt tgcccccttc
     3301 ctgaccaccg gccataaact gctgcggatt caggtcgccc agctgcttca gcatctggtc
     3361 ggtattcaga ccattcaggt gactctctgc ggatggcgga ttcggcacgg gacttcccgg
     3421 gttactctct tccacctgcc cctgagcctg ctccagcgcc atctcttcca cggttttcag
     3481 ctggaaaccg tcctggaaca ccaccgtcac ttcattaccc gccccgatgg gtatcaccgg
     3541 gtgatactgc tcagcgcgtt tgatgtagta gtcactgagt gtctgtgccg ctttcgatgc
     3601 accgccaccg atacccattt tcaggacatc gccggccccg taagcggcag tggcacccag
     3661 cccgacagcc ggctgggaag cgcgctccat tccctgaccg ataccgtcaa caaatcccgc
     3721 gccccatgcc cagccgagga ttttgccgtt acgcatcacc acttcaccct tgataccgtt
     3781 tttaccccgg aagctgacat gccctttaac cggcatatcg atggttttac cctccttcag
     3841 gcagctgata ttgcgggtac gcacgatggc acgctcactg gacacatccc cccaggcttc
     3901 cagcccaaca aagcatcccg ttgcgtcata ggttttgctg ttcggcattt ccaccagacc
     3961 ggtgatacgc agctgcatcg gcaccgtgga ttcattaccg gtgacagaag cattggcatc
     4021 cgccccttca atcagaatgg ctttcgcaaa actgcctgac ggaatgtacg gcagggacgg
     4081 agtttgcttt gtgccagcat catagctgaa cgttttacgc tgtatccggt tcggcaccgg
     4141 cacagactgg tacgtcacct gtggcggcgg cgtggctccg ttgcccggat agaacgcggt
     4201 gggcggtggc actgcacctg ccccctgcgg tggaaaagca acgggcgcat taccgggctg
     4261 aggctccccc tccggaccgg gaggggatgc cggcatctgt ggcacaggct ctccggtcac
     4321 tgtaacggga ttcgcgccca gggctttcac ctgctctgcc agcgcagcat tctcctgccc
     4381 cagcttttca atgcgacgct ggtcatcacc acgctgtttg ttcagaacat ccatctcacg
     4441 acggatttcc tcatactgct tctgcatctg tgccgccgtc acctgcatct ctgtggtggc
     4501 atgctgacgc accttgtcat caaaggtcgt atccacaaca ccggtcatat ccggcacagg
     4561 ctcctgccct gcaacggcat cagtgttccc ggacatatcc atatcagaca gataaagcgc
     4621 accaccaatt gctgataccg atccgacaat cacaatcccc agccacagga actgcttgcg
     4681 tttcacaatg gtgttgatac tggccatcag ttgccctccc cgtcacgaat gacgtacaca
     4741 tccatacggg cagcgaccag taactggctg acaggctggc tgaacatcac cgcccgggtt
     4801 cccggctgcc agaagtcact ttcccggata ttcagcgcag acagcgtttt attttccacg
     4861 gtaaagcgca ccatcttcag gtgattcccc gtccatacgg cttcagcttt tgcggacatt
     4921 ccggcagggg cctgcagggt ttcctttgtc acggggatct gataccagcc ttctggcagt
     4981 tgtccggcac gaaccgcctg actgatggaa accagcaggg attcgtaagg cgtggatgtt
     5041 tcccatgcac cggcagtttc tcctgttccg cgcaaatcgc tgaccagctg aatggtacgc
     5101 ccggcgcctt cacggggaac ggcctgaatg gaaagattaa gaccacgttc tgtttccaga
     5161 atgaacgtaa aaggcttttt gttgacggtg gccaccacca caccaccgga cgcagtctgc
     5221 tcattattgg tcagggcacc atccagactg ttcacggcga taatccggtc accggggacg
     5281 gtaaacagat tcgggctggt attactgatg gcaatacttg cctgaccgcc attgaccatc
     5341 ggaaccactg taggtgccag tgtgccgttt cctgccagca cgctgccgga aaaaagcagg
     5401 caggcggata ttattactgt cttatttttt ctcatcgtct gtttccccga aattatccag
     5461 ccaggtcacc ccgttttccc gtttcagaat aagaatgtaa tgtttgatat cagtgaaagg
     5521 ttcggagtta ccaatccatg ttttccgaat cccacggatt tccacacgac catattgtgg
     5581 ccagacacga acactggtct ggaaaaaagc agaattcaca ttatcttttt tgatgagctt
     5641 tgcttcttcg gccagaatca ctttcatctt attctgcgcg cccggacgga tatactgcag
     5701 aagtgcctgg tgagaagcat cgacgatttc cggtgagaca ttcaggcgca gggcaataaa
     5761 cgccagcgcc atctgctgca aaaaagaggc atcagcactg ttctgtgata ccgcaaaggg
     5821 catattaaat cccatcggtg tcacggcagt acgctgttca ttctgaaggc ggtaattatt
     5881 caccccctga atcacgttaa cagtcaggct gagaacaatg agcagggaca taaatataaa
     5941 agcaatggcc agtacacgac tggtacttaa gcgggcaccg tgttccatat aacaatccag
     6001 gtatcagttc tatttaatcc actgccggaa acacgaatca ggaacattat gaaaaatacc
     6061 gcgcagcagg actgttggca tataccagta aataagatcc cgtaaccaga agctgccccg
     6121 cccttttttc agctttttta tcccgaaata aaccagaacc gccgcaccaa taccgaacag
     6181 atatttcgat gttgtgatac cccagccaat acagatcgct gcggggatca gttcatccag
     6241 tggcaggcca aaccagcggc tctggttggt cagtgtttcc gggaaacgat atttcttaag
     6301 tttattctcg tctcccgaca tcgttttatt tcctgtcaga ggccaacgac ggtcatacct
     6361 acagcaataa atacagagat gatggcaaaa ccggccagga acttgacgtt tttggtcatc
     6421 atgtacatga ccgccccgac cagcacttca gccagaacga cccatttaac aacactggag
     6481 tccttaccaa aggtggcttt gacggtgtca ttcccggact gcatgaggtc ctgaccgcca
     6541 gcagccaggg ccatctgagg gaagaacatc atcagcacag cagccgggat cacagcacga
     6601 accaggcgaa gcaggttcag tttagagaaa aaagatgatt tctttttaac aggcgcaaaa
     6661 gcccccggaa cactaacaga agtattcatc aattacctcg gattgtatgt aaaaagatta
     6721 aaactaaagt tctttaaaag ttgattctct ctcttctgtc acctccctga atatttcttc
     6781 gttataaaaa tctggataac gttgcaaatg atcctgcaac ctcaccaatg cttcatttgt
     6841 tttcgttctt ccactgcgct ctctggctcc aagtaataat ttattggttg cctcatcaag
     6901 ggtcagtaaa acctttctac ttggagcagc tcttaaagca cctttgatca ccacacctcc
     6961 cgtacataaa agagatttag tcaaaatatt cacataaagc gcacacaaaa caaacaacaa
     7021 aatagtaaat tacgttaaca aaaaccggca cttttagtgc cgggaatact tacgtttctt
     7081 tacagtgaag gttgttaata ttactagcga agtattgata tagataaaat aacacatcag
     7141 aaattatagc atatcgcaac cactcatccc tgtctgagaa accatagtgt tttttaaaat
     7201 tcataaatat ttttttagac tggtcaatag agacatccat tctctgagca gaatactcat
     7261 gactataacc caaaagatac ggtattatct tattaaagcg atatttgtca gattcacaac
     7321 aaaactcctt gccctttata aaaaagctac taactacgta tctttcgaaa aatctccaga
     7381 taaacaaatt caagttattc acttcggtac tttcaaccac cacatcccat gatacatcat
     7441 tcaatattac accattaata atgctcactc catttgagga cgaaaaagca tcaacttcag
     7501 cagtctgaaa taaaatttgc gtatcaatat caataccaga aaaccatatt tcagaatcac
     7561 aattctgaca ctttattgag tgtaaaaaaa cagagtttga gtctatgaat tttcctgact
     7621 ctgtgcggac acagactgga tattttgcgg ataaaataat ttccggcagg cctgattgac
     7681 gtatgagtgc tctttctcta gtatccatcg cacacatagg aacctcctca aaggattcta
     7741 tggacagtcg atgcagggag ctagtccctc cctgcatcgg cgattttcaa gttcaaattt
     7801 taacgtggca ttaaccacat aattaatata ctcgttatgt ggttaattgt catcaaatag
     7861 aaccagatca atattctgtt atttgaataa aattaacttt taattctttt ttattcctca
     7921 tcattttttg gaaaaaatcg ttccatctca gatgacacct tctcccggat atcttcaacc
     7981 atattggcat attcaaactt tggatttccg gagacatgag gactgagaga ctcaataccc
     8041 aaaattttcg ctactgatga ttgtgttttt acaacgcatt caagaagtaa tttattaaac
     8101 tcaatctgat taaacgcgga ctctttacgc tccatctgag cttcatatac acgaagcccc
     8161 agttcaagaa gcattgaagc tgttgctgaa aaactgacat ctttttctct tgccccttcc
     8221 tgtcgacgct tctcaataat cgcatttatt ttttcataag catcattgct gatatacagg
     8281 ttcaccttag ccattagaga ttaccttttt tgaacataaa ataaagttaa tttcgaaaca
     8341 ccagagttca taatgcaaac agggacgcac cgctagcagc gcccctggcg gtatcctata
     8401 aaaaaataca ccgcgccgct agcagcaccc ctaatataaa acaatgtttt ttataaaaat
     8461 agtcagtgcc acccctacaa agcggtgtcg gcgcgttgct gtagcggcgt taacgacgct
     8521 gctttaaata aatcagattt aaacaatata aatccacaaa tacaactcaa tgatattaaa
     8581 cgcaaatcag caaaaacttg tttttgcgta gtgtgtggtg cttttagtgg tgagaaccac
     8641 taacctgttg agcctttttg tggagtgggt taaattattt cctgatgacg tcaccagagg
     8701 tgagaaaatg agaaaatggc ttctgacgat ctgcctgatg tttataaatg tgacctgcaa
     8761 ggctggtgat tgctttgatc ttgcaggtcg ggattacaaa atagaccctg acttgctgag
     8821 agcgatatca tggaaagaat cccgttaccg ggttaatgcc attggtatta atccggtaac
     8881 gggatatggc agtggactta tgcaggtaga ctcccagcat tttaacgaac tggcccgcta
     8941 cggaattaaa ccggaacatc tgacaacaga tccctgcatg aacatttata ccggtgctta
     9001 ttatctggct atagccttta aaaaatgggg cgtctcctgg gaggccgttg gtgcatacaa
     9061 cgcaggattc aggaaaaccg aacgccagaa ccagcggcgt cttgcctatg catcagatgt
     9121 ttaccggatt tacagcggga taaaaaacag taaggggatc cagcttcgtg catctgaaaa
     9181 gccgctttca aaaatgaaca gctcgcagaa gaattaattt gatatacatt ttaaaaaacc
     9241 ggttatgccg caaacgcggc atggaaggcc acacacaaaa atatcatatg agtgataatg
     9301 ctgactggcc gaagccccgc aaagcgggaa cccatcttat atatatccgg taactgcgca
     9361 gcctcctgcc agaccatttt tcgcgggcac aatatttaca gactaccgaa aagccagttg
     9421 atgaaattga ggcatctcag acgacatatc aatgccgcat aatgataata taagtaaagc
     9481 cccgaaaaat tttcggggct ttacttatat tatcatgact tccactctct gaactgttcg
     9541 gcaatcatcc ataatgcttt gttgattcgg atatcaccat caatacccgt aatggctctt
     9601 gtcctagtat ttttaccgga ggcacttcgc cccgacagtc caccttttat catattctcc
     9661 tgaacccgct gccaggttgt ccagagatcg ttctgtttat cctcccaacg acggggagta
     9721 attatttgtt caggcgtcac tggtgtttta ttttcatctt catatctgac catcaatgca
     9781 gctctgccaa ataaatgttg ctcgtcactg ttaagatgta tttctttcat cgcctccata
     9841 ttatcagtga ccttatcaaa gacaccgagc acttcatacg caccctcgat aacctgcccg
     9901 acaatatcac ctttatgtgg aacgcggatt tcgccaaaat tatttccgca caccagacca
     9961 tttgtgcaga caaaacgaaa aattccgggg atcatctgat aactggatga accatcatgt
    10021 gaattaagca ggataatttc aggaacttcc tgcccgttaa tatgctcttc cctgcggaga
    10081 cgtaacatat gtttactgta ttcgcggcgt cccaaatccc gaacccgact ctgacatgca
    10141 aagaacggct ggaaaccttc atcacgtaac ttattgatga tattaatggt tggaatatat
    10201 gtataacgct cactccggga ttcatgttta tcaccggaaa agacagacgg cacaaactgc
    10261 attaattcat catccgttaa aggacgctca cggcgaatgg aattataccg accaaaacgg
    10321 gaagctaatc gcataacagt aactccttta tgaaggtata aaattaataa aaattttttc
    10381 gcgtaatatc atcctccccc ggttactgtt aacaataaga taacaattcc gagggaaagg
    10441 accatcaaat caggtcagtt cgggactgac caacctgccg ggtgaaaagc agtaacagcg
    10501 cgatcagctc atcagcgttt tttctgacaa gaggggtatg attctccagg ctatccagta
    10561 agtcatgcag ctcattattg acctggtgga gcaccttaaa aagcgcccct gaatcctggt
    10621 tgctctgata ggcgagactg gccttaacag cgttattctg catgatcccg atttgtgcga
    10681 ttgcgtcttt ctgctgatcg ccggttggct gataagtggt gttaaaagaa aatgcgctca
    10741 tgtttcctcc tgtgtttaat gttttc
//
LOCUS       DAZKHK010000152        10605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKHK010000152 DAZKHK010000000
VERSION     DAZKHK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.153"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            178..2319
                     /locus_tag="ACHOS5_004321"
     CDS             178..2319
                     /locus_tag="ACHOS5_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFP9779044.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANSATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGYGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2383..4275)
                     /gene="parE"
                     /locus_tag="ACHOS5_004322"
     CDS             complement(2383..4275)
                     /gene="parE"
                     /locus_tag="ACHOS5_004322"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9779045.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4304..4885)
                     /gene="yqiA"
                     /locus_tag="ACHOS5_004323"
     CDS             complement(4304..4885)
                     /gene="yqiA"
                     /locus_tag="ACHOS5_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP9779046.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4885..5712)
                     /gene="cpdA"
                     /locus_tag="ACHOS5_004324"
     CDS             complement(4885..5712)
                     /gene="cpdA"
                     /locus_tag="ACHOS5_004324"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP9779047.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRSHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5737..6159)
                     /locus_tag="ACHOS5_004325"
     CDS             complement(5737..6159)
                     /locus_tag="ACHOS5_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP9779048.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6160..6789)
                     /gene="nudF"
                     /locus_tag="ACHOS5_004326"
     CDS             complement(6160..6789)
                     /gene="nudF"
                     /locus_tag="ACHOS5_004326"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP9779049.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6994..8475
                     /gene="tolC"
                     /locus_tag="ACHOS5_004327"
     CDS             6994..8475
                     /gene="tolC"
                     /locus_tag="ACHOS5_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP9779050.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8623..9294
                     /locus_tag="ACHOS5_004328"
     CDS             8623..9294
                     /locus_tag="ACHOS5_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP9779051.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9300..10460
                     /locus_tag="ACHOS5_004329"
     CDS             9300..10460
                     /locus_tag="ACHOS5_004329"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9779052.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAENDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2688 a         2810 c         2594 g         2513 t
ORIGIN      
        1 cataaaaaca catgtagcaa ttttttgtta tttctcattt actactacat tactgcgtga
       61 ttgaatgatt ctcaattaca tatccgatgg cgaaataaca aacggatagt gcttagtgag
      121 caataagaaa caattttcac tcgcgacaat ccgcgagttt ttgggataac agactaaatg
      181 gctatgttca caccttcatt ctcaggactc aaaggtcggg cgctcttttc actgcttttt
      241 gcggcaccga tgattcatgc aacagactct gtaacgacca aagatggcga aacaatcact
      301 gttacagcag atgcaaatag cgcaactgag gcaaccgatg gttatcaacc tctgagcacc
      361 tctacggcga cattaaccga tatgccgatg ctggatatcc cgcaggtggt caatacggtt
      421 agcgatcagg ttctggaaaa ccagaatgcg acgacgctgg atgaggcgct ttataacgtc
      481 agtaacgtgg tacagaccaa tacattaggc gggactcagg atgcttttgt acgccgtggg
      541 tttggcgcaa accgggatgg ctccatcatg accaacggtc tgcgaaccgt acttcctcgc
      601 agtttcaacg ccgccacaga acgtgtagaa gtgctaaaag gcccggcctc cacgctgtat
      661 ggcattctcg atcctggcgg actgattaac gtcgtgacca agcgcccgga aaaaacattc
      721 catggttcgg tttcagccac ctcctccagt tttggtggcg gcactgggca acttgatatc
      781 acaggtccca ttgaaggcac tcagctggcg tatcgcctta ccggggaagt gcaggatgaa
      841 gattactggc gaaacttcgg taaagagcgc agtacattta ttgccccgtc actcacctgg
      901 tttggtgaca atgcaacagt aaccatgctc tattcccatc gggactataa aactccgttc
      961 gatcgtggaa cgattttcga ccttacgacg aaacagcccg taaacgttga tcgaaaaata
     1021 cgttttgacg aaccgtttaa tattacagat ggtcagtccg atctggcgca actcaacgca
     1081 gaatatcatc tcaatagcca gtggacagcg cgctttgatt acagctacag ccaggataaa
     1141 tacagcgata atcaggcgcg tgttaccgcg tatgatgcaa cgacaggaac actgacacgg
     1201 cgtgttgatg caactcaggg atctacccag cgtatgcatg ctactcgtgc ggatctgcaa
     1261 gggaatgttg atattgccgg attctataat gagattctgg gtggggtgtc atatgaatat
     1321 tatgatcttc tgcgtacaga tatgattcgc tgtaaaaaag ctaaagattt caatatctac
     1381 aaccctgttt atggtaatac cagcaaatgt acaacggttt cggcgtcgga cagtgatcag
     1441 acgatcaaac aggagagcta ctcagcgtat gcacaggacg cactctatct gaccgataac
     1501 tggattgccg tcgccgggat ccgctatcag tattacacgc agtatgcggg taaaggccgt
     1561 ccttttaatg tcaatactga cagccgcgat gaacaatgga ctcccaaact ggggttggtc
     1621 tacaaactga cgccatcggt atccttattt gccaattatt cgcaaacatt tatgccacag
     1681 tcgtcaattg ccagctacat tggcgatctt ccaccagaat catctaatgc ttacgaagtc
     1741 ggggcaaaat tcgagctatt cgatggtatc accgcagata ttgcgctgtt tgatatccat
     1801 aaacgtaatg tgttgtatac cgaaagtgtt ggtgatgaaa ccattgccaa aacggcaggc
     1861 cgtgttcgtt caagaggggt agaagtcgac cttgcgggag cattaactga aaacattaat
     1921 atcattgcca gctacggcta taccgatgct aaggttctgg aagatcctga ttatgcaggt
     1981 aaaccattgc cgaatgttcc tcgtcatacc ggttcgctat tcctgaccta tgacattcat
     2041 aacatgcctg gcaataacac actgacgttt ggcggtggtg gatatggcgt aagccgtcgt
     2101 tcggcaacca atggggctga ctattatctg ccaggctatt ttgttgccga tgccttcgcc
     2161 gcatacaaaa tgaaattgca gtatccggtc acactgcaat taaacgtcaa aaatctgttt
     2221 gataaaacgt attacacctc ttccatcgcc acaaataatc tgggcaacca gattggcgat
     2281 ccgcgtgaag tgcaattcac ggtgaaaatg gaattttgat caaaaaagcc cggtatacac
     2341 ttgtataccg ggctatgttt tattcttctg gcgggaggat tatcagacct caatctctgc
     2401 catgtcgcct ttctcttgca accagttgcg gcgatcttcc gagcgtttct tcgccagcag
     2461 catatccatc atcgcgtcag tacgctgatc gtcttcatca tcgatagtca actgcaccag
     2521 acggcgagtg ttcggatcaa gcgtggtttc gcgcaattgc atcgggttca tttccccgag
     2581 acctttaaaa cgctggacgt ttggcttgcc tttcttgcgt tttaattgct caagtacgcc
     2641 ctctttctct tcttccgtca gcgcgtaata aacctctttc ccgaggtcaa tacggtagag
     2701 cggtggcagt gcaacgtaaa cgtgaccgtg tttcaccaat gcgcggaagt gttttacaaa
     2761 caaagcgcag agcagcgtgg caatgtgcag accatcggag tcagcatccg ccaggataca
     2821 gatcttgccg taacgaagct ggctcaaatc gtcgctgtca ggatcgatac cgatagctac
     2881 cgaaatatcg tgcacttcct gcgaagccag cacttcgtcg gaagagactt cccaggtgtt
     2941 aaggatctta cctttcagtg gcatgatcgc ctgatattcg cgatcgcgcg cctgcttggc
     3001 agatccgcct gcggagtcac cttccacaag gaatagttcg gtacggttaa gatcctgcgc
     3061 ggtacagtca gccaatttac caggcagcgc cgggccgctg gttagctttt tacgcaccac
     3121 ttttttagcc gcacgcatac gacgctgggc gctggaaatc gccatctctg ccaacagctc
     3181 cgccgcctga acgttctggt tcagccacag gatgaaggca tctttcacca cgcccgaaac
     3241 gaatgccgca cactgacgcg aagagagacg ttctttggtc tgcccggcaa actgcggatc
     3301 ctgcattttt actgacagca cataggcgca gcgatcccag atatcttccg ccgacagctt
     3361 tacaccacgc ggcagaatgt tgcggtattc acagaactca cgcatcgcgt ccagcaggcc
     3421 ctgacgcagg ccattaacat gggtaccgcc ctgcatcgtt gggatcaggt tgacgtagct
     3481 ttcggtcagc agttcaccgc cttccggcag ccacagtagc gcccagtcca ccgcttcagt
     3541 atcgccagcg aaattaccga taaacggttt ttctggcagc gtcggtaaac cgtttaccgc
     3601 ttccgccagg taatcattca gaccgtcctg atagcaccag cgctgttcgg tgttgttgat
     3661 ctcatcttta aaagtgatct caacgcccgg gcacagtacc gctttggctt tcagcacatg
     3721 cgtcaggcgt gaaacagaaa aacgcgggct gtcaaagaag gtttcatccg gccagaagtg
     3781 cacgctggta ccggtattgc gtttaccgca agtgccgaca acctgtaaat cttgcacctt
     3841 ttcgccattt tcaaaggcga tgttatagat ctgaccatcg cggcgtacgt taacttctac
     3901 gcgcttcgac agggcgttaa ctaccgaaat ccccacgcca tgcaggccgc cagagaactg
     3961 gtaatttttg ttagagaatt taccgcccgc gtgcagacgg caaagaatca gttcaaccgc
     4021 cggtaccccc tcttccgggt gaatatctac cggcatcccg cgcccatcgt caatcacttc
     4081 taacgactgg tcggcatgta agattacatc cacgcgtttt gcgtggcccg ccagtgcttc
     4141 atcgacactg ttatcaataa cttcttgccc caaatggtta gggcgagtgg tatcggtata
     4201 catccccgga cggcggcgaa ccggctcaag cccggtgagt acctcaatgg catcagcgtt
     4261 ataagtttgc gtcatggttt aagttagcaa ttcgagttga tcgtcagaga tggtgcagac
     4321 caagaaaatc gacgatcggg ttgaaataat cttcgaagcc cgtgaatgcg tggttgccgc
     4381 cttctatgac agtctggcgg caggaagcgt agtacgccac cgcctggcgg taatccagca
     4441 cttcatctcc cgtctgttgc agcagccaga tcaaatccgg cgcttccagc gggtcaatct
     4501 gcatgacttt gagatcgtaa atatggcgtg actctagcac atattgctgc ccggtgtagg
     4561 ggttctcgtt ctgaccgaga tagtccgtca gcagttcaaa cgggcgcacc gccgggttga
     4621 ccaccactgc gggcagcata aaacattgcg acaaccaggt ggcgtaatat ccccccagtg
     4681 acgaaccgac aatacccagc gaatcgccgc catgttccag gacaatggat tccagcagtt
     4741 ctgccgcgtc agaaggatac gggggcaact gcggaatgat catctcaacg tcagggtgat
     4801 gttccgccag ccagtttttt aacaagctcg cttttgcaga gcgcggcgag ctgttgaaac
     4861 cgtgtaaata aagaagcgta gacatcagta gccttctgaa gcggtatcag gttggaagcg
     4921 tgcgtccgcc aggcgatgca cctcggtggt cagcgtgcca tcagcatgta actcgagagt
     4981 acgccagccg ggcgcgatgg tgtccagcgt aaagttagaa caatgcggct taaactgcac
     5041 acaggtcgac ggcgttgcca gcaagcggcg accgttccag tcgagatcca gctcctgatg
     5101 aatgtgaccg cacagcaagt atttgacgtg cggaaacttc gccagcacgg tatccagttc
     5161 gcccgcgtta cgcaggctgt gttgatcaag ccaactacaa cctgcaggta gcggatgatg
     5221 atgcagcagc agcaacgtat ggcgttctgg cgcatcggcc agtttacgtt ccagccactc
     5281 aagctgaaac tcgctcagct caccgtgcgg cacgccaaac acctggctat ccagcaacag
     5341 gatttgccat tgctcaccaa taaacacgcg cttcgccggg gagatacccg catcctgtaa
     5401 cgcactgtac atcgcgggct ggaaatcgtg gttgcccggc agccagacgc agggcgcacg
     5461 aaaacttgcg atgccttcag cgaaatgctg ataggccgca gaggattgat cctgcgctaa
     5521 atcacctgtc gcgacaatca ggtcgaattc gtgctggtgt gaccgaatcg cctccagcac
     5581 cgcctggtag ctttcccagg tgtttacccc taacagggct tcgtgctttt gtgcaaacag
     5641 gtgagtgtcg gtaatttgta aaatcctgac tctggcctca ccagccagag gaagggttaa
     5701 caggctttcc aaatggtgtc cttaggtttc acgacgctaa taaaccggaa tcgccatcgc
     5761 tccatgtgct aaacagtatc gcaaccagtc cgctaaaaac tgattaattt gatgcttttc
     5821 gtcgcgttga tgcaactttt tattaggata atcataccgc gctttgaagc gaaaaatctg
     5881 ctggcttgaa cacacttcag ccaccatcgc gtcatgatac agacgcaccg tcattgacgg
     5941 aaggctccag taactgatcg cgggcgcagt ctgttctatt gtcaccaggg tagtgtatcg
     6001 ggtcgattcc acaatcgtca gccgatattg tgcgtttgcc acctgatagc ttacagtttc
     6061 gccgggtgcg tcattgcgcg gtaacaaacg gcgcaattgt gaaaagttca tctcgcacag
     6121 gcgcatcatt tcaggaaagt caggtgtgta acgcttcatt tatgcccact cattttttaa
     6181 cgcttgatga tgcagctgca gccattgcaa agcgatgacc gacgctgcgt tgtcgatttt
     6241 cccctcttct acccactggt atgcctgttc ccggcttacc acatgaacgc gaatatcttc
     6301 gttttcatca gccagaccgt gaataccgct tgcggtcgtg gcgtccactt cgcccaccat
     6361 aattgacgaa cgctcactgg tgccccccgg gcttgccagg aaacttaaca ccggtttggt
     6421 ccgtttgact atcagtcccg cctcttcaat cgcttcgcga cgggcaacat cttccacact
     6481 ttcaccctct tcaatcatcc cggcaaccat ctccagtagc cagggggttt cgctggtgtc
     6541 gtacgcggca atccgaatct gctcaatcag cacaacttca tcacgtactg ggtcaaaggg
     6601 tagcaagact gcggcgtgac cgcgctcaaa aatttcccgc cgtacctcat gactcatttg
     6661 cccgttgaat agacgatgac gaaatctata aagatctaat gaaaaaaagc cgcgataaag
     6721 tgtttctcgt gcaataattt ctacatcgtt tttgccaaat gtaacgggca ggttgtctgg
     6781 cttaagcatt gttaatgtcc tggcactaat agtgaattaa atgtgaattt cagcgacgtt
     6841 tgactgccgt ttgagcagtc atgtgttaaa ttgaggcaca ttaacgccct atggcacgta
     6901 acgccaacct tttgcggtag cggcttctgc tagaatccgc aataatttta cagtttgatc
     6961 gcgctaaata ctgcttcacc acaaggaatg caaatgaaga aattgctccc cattcttatc
     7021 ggcctgagcc tttctggttt cagttcgttg agccaggccg agaacctgat gcaagtttat
     7081 cagcaagcac gccttagtaa cccggaattg cgtaagtctg ccgccgatcg tgatgctgcc
     7141 tttgaaaaaa ttaatgaagc gcgcagtcca ttactgccac agctaggttt aggtgcagat
     7201 tacacctata gcaacggcta ccgcgacgcg aacggcatca actctaacgc gaccagtgcg
     7261 tccctgcagt taactcaatc catttttgat atgtcgaaat ggcgtgcgtt aacgctgcag
     7321 gaaaaagcag ctgggattca ggacgtcacg tatcagaccg atcaacaaac cttgatcctc
     7381 aacaccgcga ccgcttattt caacgtgttg aatgctattg acgttctttc ctatacacag
     7441 gcgcaaaaag aagcaatcta ccgtcaatta gatcaaacca cccaacgttt taacgtgggc
     7501 ctggtagcga tcaccgacgt gcagaacgcc cgcgcacagt acgataccgt gctggcgaac
     7561 gaagtgaccg cacgtaataa ccttgataac gcggtagagc agctgcgcca gatcaccggt
     7621 aactactatc cggaactggc ggcgctgaat gtcgaaaact ttaaaaccga caaaccacag
     7681 ccggttaaca cgctgctgaa agaagccgaa aaacgcaacc tgtccctgtt acaggcacgt
     7741 ttgagccagg acctggcgcg cgagcaaatt cgccaagcgc aggacggtca cttaccgact
     7801 ctggatttga cggcctctac cggaatttct gacacctctt acagcggttc aaaaacccgt
     7861 ggtgccgctg gtacccagta tgacgatagc aatatgggcc agaacaaagt tggcctgagc
     7921 ttctcgctgc cgatttatca gggcggaatg gttaactcgc aggtgaaaca ggcccagtac
     7981 aactttgttg gtgccagcga gcaactggaa agcgcgcatc gtagcgtcgt gcaaaccgta
     8041 cgttcctcct tcaacaacat taatgcttct atcagtagca ttaacgccta caaacaagcc
     8101 gtagtttccg ctcaaagctc attagacgcg atggaagcgg gttactcggt tggtacgcgt
     8161 accattgttg atgtgttgga tgcgaccacc acgctgtaca acgctaagca agagctggca
     8221 aatgcgcgtt ataactacct gattaaccag ttgaatatta agtcagctct gggtacgttg
     8281 aacgagcagg atctgctggc actgaacaat gcgctgagca aaccggtttc cactaatccg
     8341 gaaaacgttg ccccgcaaac gccggaacag aatgctattg ctgatggtta tgcgcctgat
     8401 agcccggcac ccgtcgttca gcaaacatcc gcacgcacta ccaccagtaa cggtcataac
     8461 cctttccgta actgacgacg acgacggggc ttcggccccg tctgaacgta aggcaacgta
     8521 aagatacggg ttatctgccg cattcttccc ccttctcgct tcaatttcga ccagccatcc
     8581 tctattctga tgggtattta ccactggtcc cggaagacaa aaatgaaacg gacaaaatcc
     8641 atacgccacg catcgttccg caaaaactgg agcgcacgcc atctgacacc agtcgctctc
     8701 gcggttgcca ctgtttttat gctggctggc tgtgaaaaga gtgatgaaac agtgtctctc
     8761 tatcaaaatg ctgacgactg ttcagctgca aacccaggca aaagcgccga atgtaccacc
     8821 gcgtacaaca atgcgctgaa agaagccgaa cgtactgcgc cgaaatacgc cacccgtgaa
     8881 gactgtgttg ctgaatttgg tgaaggtcag tgccagcagg caccagccca ggctggcatg
     8941 gcaccagaaa accaggcgca ggcccagcaa tccagcggga gtttctggat gccgctgatg
     9001 gccggttaca tgatgggacg tctgatgggc ggcggcgcgg gatttgcaca gcagccgctg
     9061 ttctcctcga aaaacccggc cagcccggct tacggtaaat ataccgacgc gaccggtaaa
     9121 aactatggcg cagcccagcc aggccgcacc atgaccgtac cgaagacggc aatggcacca
     9181 aaaccggcga ccactactac tgttacccgt ggcggttttg gtgaatctgt tgccaaacaa
     9241 agcactatgc agcgtagcgc aaccggcacc tcttctcgtt caatgggtgg ctgataccga
     9301 tggaaagagt cagtattacc gagcgcccgg actggcgcga aaaagcccac gaatacggtt
     9361 tcaattttca caccatgtac ggcgagccgt actggtgtga agatgcttac tacaagttga
     9421 ccctcgccca ggttgaaaag ctggaagaag tcaccgccga actgcaccag atgtgcctga
     9481 aagtggtgga aaaagtgatc gccagcgatg agctgatgac caaattccgc attccaaaac
     9541 acacctggag ttttgtgcgc cagtcatggc tgacgcacca gccatcgctt tattcgcgtc
     9601 ttgatctggc gtgggatggc actggcgaac ctaaacttct ggaaaataac gccgatacgc
     9661 caacgtcact atacgaggcg gcgttctttc agtggatctg gctggaagat cagcttaacg
     9721 ccggtaactt gccggagggc agcgaccagt ttaacagttt gcaagaaaag ctgatcgatc
     9781 gcttcgttga gctgcgtgaa cagtatggct tccagttgct gcatctcacc tgctgtcgcg
     9841 acacggtgga agatcgcgga accattcagt atttgcagga ctgcgcgacg gaagctgaaa
     9901 tcgctaccga gttcctctac atcgacgata tcgggttagg tgaaaaaggt cagttcacgg
     9961 atttacagga tcaggtgatt tccaacctgt tcaagctgta tccgtgggaa tttatgttgc
    10021 gtgagatgtt ctccaccaag ctggaggatg caggcgtacg ctggctggaa ccggcgtgga
    10081 agagcattat ctccaacaaa gcgcttctac cgctactgtg ggagatgttc ccgaatcacc
    10141 cgaacctgct gcccgcttat tttgcggaaa atgatcatcc gcaaatggaa aaatatgtgg
    10201 ttaaaccgat cttctcccgt gaaggcgcaa acgtgtcgat cattgagaac ggcaaaacca
    10261 ttgaagcagc ggaaggtccg tatggcgaag aagggatgat tgttcagcaa ttccacccgt
    10321 taccgaaatt cggcgacagc tatatgctga ttggtagctg gctggtgaac gatcaacctg
    10381 ccggaattgg cattcgtgaa gaccgtgcat tgatcaccca ggatatgtct cggttttatc
    10441 cgcatatttt tgttgagtaa tacagcttaa tattgccccg ataagcgggg caatctctta
    10501 tcatccacca ttcataattc ccatcaatat ataaacacat acatatccaa cctttattta
    10561 acattaatac tcaatttaat gttaactcgt catatgcaag cgata
//
LOCUS       DAZKHK010000153        10319 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKHK010000153 DAZKHK010000000
VERSION     DAZKHK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.154"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..285)
                     /locus_tag="ACHOS5_004330"
     CDS             complement(<1..285)
                     /locus_tag="ACHOS5_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9779053.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLYALSEQQKAACVALNPEKSHPYIEDMAMFLSPLPVVLEWHC"
     gene            complement(282..665)
                     /locus_tag="ACHOS5_004331"
     CDS             complement(282..665)
                     /locus_tag="ACHOS5_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9779054.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASRHCRQC
                     HHDYYGERYCTHCHTGHFSEEAVAE"
     gene            complement(833..1402)
                     /locus_tag="ACHOS5_004332"
     CDS             complement(833..1402)
                     /locus_tag="ACHOS5_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP9779055.1"
                     /translation="MTYKYNPFWQQRIREMVLCALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDTGDHTTLAHFPDTPAVWLERDDDTSFQ
                     QVLERADYLAKECTKAYGTGERNFGCSRG"
     gene            1601..1795
                     /locus_tag="ACHOS5_004333"
     CDS             1601..1795
                     /locus_tag="ACHOS5_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779056.1"
                     /translation="MVIRKKKCRDCDNAITHNTMCCPYCGAVDPFGYYRNTDRIFTAL
                     LALLVVVLLVTSAVSMYVLW"
     gene            3248..3454
                     /locus_tag="ACHOS5_004334"
     CDS             3248..3454
                     /locus_tag="ACHOS5_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9779057.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3542..4150
                     /locus_tag="ACHOS5_004335"
     CDS             3542..4150
                     /locus_tag="ACHOS5_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779058.1"
                     /translation="MKMKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHN
                     LTPGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPML
                     EGILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDNWTENLK
                     RKSEKELEEWIIDRQKQNAALTDLMDQYVLLAYRTTVDDNRN"
     gene            5347..6264
                     /locus_tag="ACHOS5_004336"
     CDS             5347..6264
                     /locus_tag="ACHOS5_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779059.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYEPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRNEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKDKNIALENLASFPGSADIYHA
                     IHLSFDIWVTHPSALPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRARRLSTGEMLHDLIVEEYKRYRHSR"
     gene            6348..7221
                     /locus_tag="ACHOS5_004337"
                     /pseudo
     CDS             6348..7221
                     /locus_tag="ACHOS5_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            7439..9823
                     /locus_tag="ACHOS5_004338"
     CDS             7439..9823
                     /locus_tag="ACHOS5_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP9779060.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPILHLEHIQPVRNLLSTLQEKLATPAGQQIAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADTVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKDYVCKDSELATEGATDL
                     WEGSLIDQLITEVILSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIREIADCQEQVTTDVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTRDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDRKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            9820..>10319
                     /locus_tag="ACHOS5_004339"
     CDS             9820..>10319
                     /locus_tag="ACHOS5_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9779061.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGMTGEGAARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGR"
BASE COUNT         2612 a         2657 c         2586 g         2464 t
ORIGIN      
        1 acaatgccac tccagcacga ccggtaatgg cgataaaaac atcgccatat cctcaatgta
       61 agggtgggac ttctccggat tcagcgccac gcaggccgcc ttctgttgct cgctcagggc
      121 atataaatca tgctcaaacc acgccccctg agcatctgtc tgcaaaatca accggccatg
      181 acaggaaagg cagaaacact gccggatatt tctgctgagc gcagaatcag cactgataat
      241 gtgttcaccc ggcgtgatcc ccagccccgt ttttataccg ttcattctgc cactgcctcc
      301 tcactgaagt gcccggtatg gcagtgtgtg cagtaccgct ccccataata atcgtgatga
      361 cattgtctgc agtgccggct ggctttacgc accacgggta aggcatccgg tacgaattgc
      421 tgcagacgtt taatcaactg tatttctctg cgctccggcc tgacataagg gcactgttga
      481 ccgtgctctg tcagcctgtc gtcagtgtgt tcaaaccagg gaagttcagt gtcgtattgc
      541 gggtggtatc tgagcgcact gccgcaaagg tggcaggtgt agcgatcgta aggtgcagtc
      601 tgtgcggtac gggcccccgt cagacgtccg ttaccatcaa gtgcgagaaa ggattttgcg
      661 tacatagtat atattcctca gccgccagac gacacgcagg caacagtcgt cccggtacgg
      721 gcagcgtggt caggtgtgaa tggcggtttt cagttcagtc tcagaaggga aaatcagtgt
      781 ggtcgctgtg aacacgtcat cacactaagg tcagataagt cgtctgtcgg gctcagccac
      841 gactgcagcc aaagttgcgc tcaccggtgc cgtaagcttt ggtacactcc ttcgccagat
      901 agtcagcacg ctccagcacc tgctgaaagc tggtgtcatc atcgcgttcc agccacaccg
      961 ccggcgtgtc aggaaaatgc gccagcgtgg tatgatctcc ggtatccacc cccagggcac
     1021 tgcaccacgc ctgtttaatc atcccggcca gtgaccccgg agcgcggtaa tcaccggcac
     1081 gacaccaggt atcccggttg accagcagca tcaggtgata gtgtttttta cctttgcact
     1141 ccccaaactc ccgggcccag acgtaatgca gggtcgtggg atgtacgcgt ttaccttcac
     1201 gccgcttacg cttctggtaa gcatcgattc gggctttcag ggcattgatg aagcgggata
     1261 tcacagccgc atccgtagct gccggtacat ccgggagacg cagatcaacc cgaagtgccg
     1321 ttaggcgggg atgaacattc agtgcgcaca gcaccatctc acgaatacgt tgctgccaga
     1381 aggggttgta tttgtaggtc atggttaaat ctccgtatgg ttcatacgga atagccacgt
     1441 cgtaaaaaat gcgcagagtc cctgaagtga cctccggcag gacactgtct caggcactct
     1501 gtgataaccc gaacggtcat cgccggactg acgattgaat tcccggcgtt gtttttttaa
     1561 tgtaaaaaac aggttacgga taaggcgtgg agagggagcg atcgtgatac gcaaaaaaaa
     1621 gtgcagggac tgtgataacg cgattactca taatacgatg tgttgtccgt attgtggcgc
     1681 tgtcgatccc ttcggttatt accggaatac ggacaggatc ttcacggccc tgctggcgct
     1741 gctggtagtg gttttgctgg tgacatctgc tgtcagcatg tatgtgctgt ggtagcggta
     1801 acgggtctca tcagatatcc gtgagggtgg cctgactccg tgagactgca acgcgccgtc
     1861 aaccctcacg ctgtagagct cccgtactcc ggatgtcgcg ggattcctgt gggcgcagcc
     1921 cctggcggga acacttttaa cctccgcggg caaccgcgtg atgcgccaca acaacgcgct
     1981 acaggccggt aatatccgaa acagaccacc ctgccccttt taatttcaaa ttattcgttg
     2041 ccacaatacc gcttaacgtt cgcggtatta tccaggttac caacaacctg agaagacctc
     2101 aaaagaatca cacagagcag ataacaccag accgttgtca ttaccggata ctcaggtaac
     2161 cagactgaat ccacacgcgt aaagtttgtc gtcagttctg ccagccgatc gaccgcagaa
     2221 ggcttccggc tccgcagagg tatgaggtta taacgtgtgc gggtaatcac tttttccggc
     2281 gtatcccggg atgaacaacg acggaactgc tctggtttta acttccattc gtctctcgtt
     2341 actttatgct gtaatcaacg tgatactgcc agcataccgc accagactgg cattctggtg
     2401 ccctccccaa actcattact cccgtgccac cacttaaccg gtctctcagc aatatcgcct
     2461 taacgctacg tttagttatc ccacaccgga tgtccgatgc cattgcgaca acctgaaggt
     2521 aaccagtcag ttagctgagg tgttaagctg ctggatgcat acaccttgca tacaccttgc
     2581 caaatctaat cgctcttaac caatttccag gatacaaggg ggcgattact gagaatgctc
     2641 tcagactgtc actcaccaga tcggatacgt ttaccgctca gacggatgct taatgcactc
     2701 tgttcacgtg tgttgcaggt acgttttata tacgcaggtt atacgccagc tatcagagat
     2761 agccctgtgg gtacaacgca atctgctgct actgcgttct catgcccact gatcgcactc
     2821 acagtgttta tttcacatat atgaactcat gtatttgatc tcatgtgttt gatctctctt
     2881 atatatttat aataccaatc agacagtctt atcccaccag tggtacctgt cgcctgccca
     2941 caggtcacag gctgatgcac cacaaccgcc ttcaaaaaca actctgcaat tatcagaacg
     3001 ccagttttcc ccttgtctca cgaaaatcct ccctccttta tcgcgtgcgc gtgcggaagc
     3061 atcgtttcgc aacaatcacc cgacataccg gtacggctca gccaccgcaa tccccccgtt
     3121 tatctcctga cggtcacatt cccgattatt ttttacgttt ctccccggtt gttatgccgg
     3181 ggaaggtggt gcgttgtttt catcaccaac gccggttgag attaacaaca tccggaggaa
     3241 cattctcatg accaccccag tttcactgat ggatgaccag atggtcgaca tggcgtttat
     3301 cactcaactg actggcctga ccgataagtg gttttacaaa ctcatcaggg atggggcctt
     3361 tccggccccc atcaagctgg gccgcagttc ccgatggctg aaaagtgaag tggaagcctg
     3421 gctgcaggcg cgtattgcac agtcccgtcc gtaatttctg accgttatcc gttcacccgc
     3481 agcagacgta atccccggcc tgccggcggc attctgctgc ctgttatccc ccgtgaggaa
     3541 tatgaaaatg aaaccacagt atcagacccg ttacgaactg ctccacgaaa gctaccagaa
     3601 atggctgacc ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcta
     3661 ctacttccat aatctgacgc ccgggtgggt gtcattcaat ggtgaaaaac cggagattgc
     3721 catcgtaccc cagagcctgc accggctgat ttatggccct gacaaacggg ccacgccgcc
     3781 cctggatgat gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc
     3841 gatgctggaa ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt ctctggcaaa
     3901 taaactgatc agcctcttcc gtcagtttgg tggcacggaa ttgcgcctca aactggtctg
     3961 gctttgctgg cttgatttaa tgaccggaaa cagccttgac aactggacgg agaacctgaa
     4021 acggaaatca gaaaaagagc tggaggaatg gatcatcgac cgtcagaagc agaacgcagc
     4081 gctgacggat ctgatggatc agtacgtgct cctggcatac cgcacaacgg ttgacgataa
     4141 ccgcaactga tgccggttgc ttccggaagg tgcggcaaaa tacgggaatg ctgctcccgg
     4201 ccccgcatgc tcacctgcag agcgtgacat cgtcatcata ctgagccatg acgggcatct
     4261 taagggcatc ctgtcattgc tgagcaggca acaccaccag aggagccacg gacagggatg
     4321 ttctttacag cccgatacac gccaggttct cagcagactg aatgtgcagg tgcggttatt
     4381 caccagaaat ggcatgctgc ggtacatacc catcctggtg acaatatgta cgtggcgaca
     4441 gcgatcccgg cactgtggaa gagactcact cagcctgcag gatattcttt tcggcatcca
     4501 ggaatgtctg cctgaatgca aacaggatga tatttgaatc cgtaatactg ttctttcatc
     4561 ataaataatt tatgcagata cagttaaccg tcacagtagc aggtttgttt tcccgggaaa
     4621 taatatccgt cagactgatg ccggaacata tctgaaacac gttatgatgt tttgttatca
     4681 gaaatgttgt atattcttat ccgtgacgac agtttaatgc cctctggtat cacctgataa
     4741 tgcatatcct gcgatgattc gttatattta cgggacacgt ctcttttcat tgcactcctt
     4801 ttcagacttc attttccgta agtgttcccc ggcataaagg aggtaccttt ctggcgttta
     4861 taactctctt tatcggtgac gccggtaata cagggaatac cacaccgaca aacgaggtat
     4921 taacagacga tatttcagtg tccataattt tgctgtccgg ttaaataacg tcagtctgct
     4981 gacctctgac tgtaatcatc aaagccggaa tacataccgg gttacaacgt aaaaaaagtg
     5041 atgctggcct ccggtgcagc tggcgggcga aacatgttta cctttcaata ccccctgtct
     5101 gcagtctcca ctcttccatc ctcttaagct acgtcggcca catgaatgca cttccatgat
     5161 aacaggtatt cctgtcagca taacgaagcc ggttaccttc ccttcctcct gcatttcctg
     5221 tcataatgac tatacagcgc gcagattccc tttttattga ccaccttaat ataccattat
     5281 aaacttcttt ttatcatgtt attatcacct tattatgttt aatttttaag caacaggaga
     5341 aagaggatgg ttcagaaaat tttgtcggat aaggttatga atgaaagaac aaatgcgtat
     5401 tactcttatt atcttggaga gcgtaatatc tccgtactgc cccttaatgt ttatgagccc
     5461 ccggaacgtt ttatcgctta cataaaaaaa aacagagaaa atctgaacat cacactttca
     5521 gattttgaac ttgaacaaat aatttctggt atgcgtctga aggctctggc cttcctggtc
     5581 cccctggaaa aaatatcctg gatcgcgggc agtgaacgcg catgcttatt ttcctggtat
     5641 ctgttgatgc agtttatcca gaataacagg gcaaaaataa gcgcagactt gcttcaaaag
     5701 aataaactgt atcttaaaga agagtattta gaagggaatg ccttcccgtc ggactcatcg
     5761 acacagttca gacaaatact cagagttctc gatattcttt ctgataagaa tttgcggaat
     5821 gagtggataa tccagaccaa agacagatgg atgcgggcat ttaaatcaaa gagccctttc
     5881 agttacctcc tccctgagaa tgaacatgaa tgcatatgga catggaatta tttaaaggat
     5941 aaaaatattg cactggaaaa cctggccagt tttcctggtt ctgctgatat ttaccacgca
     6001 attcaccttt catttgacat atgggttacc catccatcag ccttacctga cgatataaaa
     6061 aatttcagga acagtttcaa taaggccaag gcacagcgca agtacaaaaa aatgcaggaa
     6121 gataaagtca atgtacagtt ttttcttgac gcagaaacca gggcgcagct caaagaatta
     6181 tcgagggcca gaagactcag taccggcgaa atgctgcatg acctgattgt tgaagagtat
     6241 aaaaggtacc gacattccag ataatttcag acatatatta tctccgtgaa tcccccgcca
     6301 cctttccggt gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg
     6361 atgccattga ggcaattgaa aaaccccttt cttccctgcc ttattcactt tcccgtcaca
     6421 tcccggaaca tctgcgcaaa ctcaccagtc acgaccccgt gcctggcatt atgggtaaaa
     6481 gcggggccgg taaatcctca ctctgtaatg ccctgtttca gggggaggtc acaccggtca
     6541 gtgatgttca cgccggcacc cgggaagtgc ggcgcttccg tctgagtggc tacggtcaca
     6601 gcatggttat cactgacctg cccggggtgg gcgagagcca ggacagggat gcagagtatg
     6661 aagccctgta ccgtgacatt ctgcccgaac tggacctggt actgtggctg attaaagccg
     6721 atgaccgtgc cctgtctgtg gatgagtatt tctggcgaca catcctgcac cggggacatc
     6781 agtgggtact gtttgtggtg atgcaggccg ataaaacgga gccctgccat gaatgggata
     6841 tggccggtat tcagccttct cccgcacagg cacagaatat tcgcgaaaaa acggaggcgg
     6901 tattccgtct gttccggccc gtacatccgg ttgtggccgt atcggcccgc accggccggg
     6961 agccggacaa cgctggtcag tgcgctcatg acagcgcttc ccggacatgc cgccagtccc
     7021 ctgatgaccc gactgcagga cgagctgcgc acggaatctg tccgcgctca ggcccgtgaa
     7081 cagtttaccg gtgcggtgga ccggatattt gacacggcgg agagcgtctg tattgcctct
     7141 gttgcacgca cggttctgca tgccgtccgt gccaccgtgg tctctgttgc ccgcgcggta
     7201 tggaactgga ttttcttctg aacccggtgg tgatgtacca ggctgttgtt acagattttt
     7261 aactccatga aaacacaatg caaatgcagc catacccggg ttgtcccctt gcgttgtgtt
     7321 ttttaaatta atagaattaa acgatcgtta ctatcatatc gatcgttata aacgatcgtt
     7381 tgtggtaaat gacctcctcc gtggttgtca tgaaaatgcg tctcaggaag gttaaataat
     7441 gcacgaaaag aacatcgccc tgctttgtga tgaagccgac cgacttttgc aactgaacat
     7501 taatctgctc cggcaaatgg ttgatgaacc ggatgtgtta tctgacagta agaacgaaaa
     7561 cagactgctt ttcgataaac agaaagcact gaaaagaatt gaggagctgg agggcgaaca
     7621 aatcaaaacc gcccgcaggg agatggtgct ggctgttgtc ggcacgatga aagcaggtaa
     7681 atcaaccacc atcaacgcca ttgtggggca ggaaattctg cctaaccgta accgccccat
     7741 gacctctgta ccgacgctca tccgccacgt tcccggaaaa actgagccga tcctccatct
     7801 ggaacatatt cagcccgtcc gcaatttatt aagcacactg caggaaaaac tcgccacccc
     7861 ggcaggacag caaatcgcac agaccctgca gcaaaccggg gatacctgcg aactgctgga
     7921 tattctggca aatgatggct ggctcaaaaa tgaataccac ggggaggagg aaatctttac
     7981 cggactggca tcgttaaacg atctggttcg tcttgctgcg gcaatgggga gtgagtttcc
     8041 ttttgatgaa tacgcagaag tgcagaaact gccggtgatc gacgtggaat tcagccatct
     8101 ggtggggatg gatgcatgcc agggaacact cacactgctg gatacccctg gccctaatga
     8161 ggccggacaa ccgcagatgg aagtgatgat gcgggatcaa ctgcagaaag cctctgcggt
     8221 tctggctgtg atggattaca cccagatgaa ctcaaaagcg gatgaagacg tccgtaaaga
     8281 gcttaatgcc attgctgacg tatcagccgg ccgcctgttt gtgctggtca ataaatttga
     8341 tgagaaagac cgcaatggtg atggtgcaga tactgtacgc cagaaggtac cggcaatgct
     8401 gaacagcgat gtgctgcccg cctcccgcgt ttatcctggc tcatcacgtc aggcgtacct
     8461 ggctaaccgt gcgcttcatg agttacggaa aaacggaacc cttcctgttg atgaagcctg
     8521 ggtcgatgat tttgtcaggg aagctttcgg tcgcatgaaa aaagattacg tctgtaaaga
     8581 cagcgaactg gcaaccgaag gggcaacaga cctgtgggaa ggctcactta tcgatcagct
     8641 gataacggaa gtcatactga gttcacattc cagagcggcg gcactggcgg ttgattctgc
     8701 cgccgcaaaa ctgatgcaga atgcggaaaa tgtcagcgaa tatctgtcat tacgccatca
     8761 ggggctacag caaagcattc agtcactgca ggcgcatatc accagcctgc tggcggatat
     8821 ccgggaaatc gcggactgtc aggagcaggt gaccactgat gtcagaatgg ctatggagga
     8881 gatcgatgcc agaacccggg aattactgac gggggtctgc acctcactgg aagaggagct
     8941 gaatgactat ttcagaagcg gtaaacgcaa agaacagcaa atgctggagg aagaaaattc
     9001 agcccagccc cgggagagaa acgcattcgc tttctttcac gatattttcg gaaccggaaa
     9061 tcagcatgac aggatgcggg actttgatcc ggacagcccc gaaataaaat tcagtgatcg
     9121 cagggaagcc cttgaactga tgacgcaaat cgaatcgacc gtgaccagcc tgcaccgtga
     9181 ggctgaagca cagttccgtc ctgaactgga gaaaatcgtc aggggtattg aaacaggttt
     9241 tcgtggtacg gccctgtacg ccacagaaaa tattgccgga cgcatcaatg cccgtctgga
     9301 ggatgaaggt ttcactgtaa aaatcagttt tccggcagtc agccagttac agacccggct
     9361 cgcagtaaaa acaaatctga gcgcgcttat ggaggaaaga acggagaccg tcacccgtcg
     9421 ctgtcgacag gatggtgtct ggggtacgct ttgtcgatgg gcaaacaccc gtgactgggg
     9481 ctggaaagag tacagtgtgg atgtgagccg cagcgtgatc aatatcaaca aggtcagaga
     9541 ggaagtgatg tcactgaccc gggcatattt cggggaactg caggcatcca ttgagcagga
     9601 tattaaccag cccgtccgcc aggaaatcga tgcctttttc tgcgcattca gggagaaagt
     9661 tgaacaactg cgtaacacgc tgattcagag ctctgaagat cgtaaacgcg atcagcaggc
     9721 gcaggaacgg cttaccgggc gacttcaggc attaaatgaa agggttcctg agctcattac
     9781 tgacagtaag gcgctgaggg aagagctgga gacaatgctg tgacttcacc atttattcaa
     9841 caaattgctg ataaccgggt atgtcaggta ctcacctgtc ttcctgaaaa atttgtggtt
     9901 gatttcgcaa acggaatcga tgttgcacag gagcatatcc gcacggccgg agagcgcacg
     9961 ttcttccggc gtttaaaaga ggggatgacc ggcgaaggcg ctgcacggca gaatgccatc
    10021 aatgcttcgc ttgcacaggg cgttgaggcg tccctgcgct ggctgacgga gctgacaacg
    10081 tcacttgcca ccacaaacta cgcgattacc cgggtaaacg acagggtcag ttcactggtc
    10141 agtgatactg ccaggctggc gcattattcg gctgacacgc gggagcagtt actcaccctg
    10201 gccgatcagg ttcaccagaa actgaatcat cttgaagaaa aactccaccg tgttgaccag
    10261 gttcagcggg cgcagttaca tcttgagcag atattctcat ggtggagcgc cggacgata
//
LOCUS       DAZKHK010000154        10291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKHK010000154 DAZKHK010000000
VERSION     DAZKHK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.155"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..636
                     /locus_tag="ACHOS5_004340"
     CDS             <1..636
                     /locus_tag="ACHOS5_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element ISEc40 family transposase"
                     /protein_id="HFP9779062.1"
                     /translation="KKPSGVPHKFSEEKQRQFIEYYKELKTTVGDEPILFIDGVHPTQ
                     ATKISYGWIRKGQKKAVKTTGSRTRLNIMGALNLKALTSPLICEYKTINEYNVSRFFN
                     EIRKVYPDYNQKIHVILDGAGYHRSQLVKDWAEVVNIRLHYLPPYSPNLNPIERMWKL
                     MNEHARNNRYFSSTREFREAISVFFNQTLPNIADSLTSRINDHFQMLTPAS"
     gene            complement(962..1513)
                     /gene="papX"
                     /locus_tag="ACHOS5_004341"
     CDS             complement(962..1513)
                     /gene="papX"
                     /locus_tag="ACHOS5_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PapX"
                     /protein_id="HFP9779063.1"
                     /translation="MRACTQTVCFSNMENNEMNNTDTLEKIIRHQKNKDPAYPFREHL
                     LMQLCIRTNKRMQDNISEFLGAYGINHSAYMVLTTLFAAENHCLSPSEISQKLQFTRT
                     NITRITDFLEKTGYVKRTDSREDRRAKKISLTSEGMFFIQRLTLAQSMYLKEIWGYLT
                     HDEQELFEVINKKLLAHLDDVSS"
     gene            complement(1660..2400)
                     /gene="sfaY"
                     /locus_tag="ACHOS5_004342"
     CDS             complement(1660..2400)
                     /gene="sfaY"
                     /locus_tag="ACHOS5_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SfaY"
                     /protein_id="HFP9779064.1"
                     /translation="MSGAVNSILEVKSGRTEYILSPVFEPVWNQTGKIFAFEMLSDIR
                     SAKDGRKICASVFFRSAPPDIQYKILISQLKTAETLHKWCLQKKIMLSVNISRVVALY
                     LRKHGIPGRPGTHIRLEISEDFPAVALKPGDDPLLRFLSERFTLWLDDFGSGNAGLMW
                     GLSGMFERVKISHEFFHYALKNRSAMPLLHAVADTVACHNRGVILEGVENEALFRIAR
                     DMNVQGCQGWLYRRVGADELSAITEQYG"
     gene            complement(2704..3714)
                     /gene="focH"
                     /locus_tag="ACHOS5_004343"
     CDS             complement(2704..3714)
                     /gene="focH"
                     /locus_tag="ACHOS5_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1C fimbrial protein subunit FocH"
                     /protein_id="HFP9779065.1"
                     /translation="MREVLIHKLLSPIPMRKYYPLFKKSTVLEGCVLFTFIMAYSQPS
                     FALLCRNNQTGQTFQSGDSSFNITLSPTVQYDKAITVLDLNQLVLCQNEDASGQNYDY
                     LRVRQGTGFSPSLDAKTYGRLDFTNRLSGYSQTLPLQQDTRPTEAYWQYGVWKPFPAK
                     MYLYPEPGVFGKLIHAGELVATVYVNKFSTMGQEAGERNFTWRFYATNDVYIQTGTCR
                     VSSNNVKVDLPSYPGGPVTVPLTVRCDQTQSVSYTLSGSVTGSGNTVFANTATSGAGG
                     VGVQLSDNAGLVPAGQPRSLGQVGSSPVSLGLKASYALTGQASPTPGAVQSVINVTFS
                     YN"
     gene            complement(3665..4168)
                     /gene="focG"
                     /locus_tag="ACHOS5_004344"
     CDS             complement(3665..4168)
                     /gene="focG"
                     /locus_tag="ACHOS5_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151062262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1C fimbria minor subunit FocG"
                     /protein_id="HFP9779066.1"
                     /translation="MKLKAIILATGLINCIAFSAQAVDTTITVTGRVLPRTCTIGNGG
                     NPNATVVLDNAYTSDLIAANSTSQWKNFSLTLTNCQNVNNVTATFGGTAENTNYYRNT
                     GDATNIMVELQEQGNGNTPLKVGSTKVVTVSNGQATFNLKVRAVSKGNAGAGSINSQI
                     TVTYTYA"
     gene            complement(4190..4717)
                     /gene="sfaG"
                     /locus_tag="ACHOS5_004345"
     CDS             complement(4190..4717)
                     /gene="sfaG"
                     /locus_tag="ACHOS5_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial adhesin minor pilin SfaG/FocF"
                     /protein_id="HFP9779067.1"
                     /translation="MVKDIIKTVTFSCMLAGSMFVTCHVCAVGSVVNITGNVQDNTCD
                     VDINSRNFDVSLGSYDSRQFTAAGDTTPASVFHVGLTSCGSAVRAVKLTFTGTPDNQE
                     AGLIQINSINGARGVGIQLLDKDKHELKINVPTTIALMPGTQTIAFYARLKATYLPVK
                     AGNVDAVVNFVLDYQ"
     gene            complement(4730..7360)
                     /gene="sfaF"
                     /locus_tag="ACHOS5_004346"
                     /gene_synonym="focD"
     CDS             complement(4730..7360)
                     /gene="sfaF"
                     /locus_tag="ACHOS5_004346"
                     /gene_synonym="focD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial usher SfaF/FocD"
                     /protein_id="HFP9779068.1"
                     /translation="MFSGDGGQLLSDKSLTGSAGGGNNRMKFNILPLVFFIGIIVSPA
                     RAELYFNPRFLSDDPDAVADLSAFTQGQELPPGVYRVDIYLNDTYISTRDVQFQMSQD
                     GKQLAPCLSPEHMSAMGVNRYAVPGMERLPADTCTSLNSMIQGATFRFDVGQQRLYLT
                     VPQIYMSNQARGYIAPEYWDNGITAALLNYDFSGNRVRDSYGGTSDYAYLNLKTGLNI
                     GSWRLRDNTSWSYSTGKGYSQNNWQHINTWLERDIVPLRSRLTMGDSYTRGDIFDGVN
                     FRGIQLASDDNMVPDSQRGYAPTIHGISRGTSRISIRQNGYEIYQSTLPPGPFEINDI
                     YPAGSGGDLQVTLQEADGSVQRFNVPWSSVPVLQREGHLKYALSAGEFRSGGHQQDNP
                     RFAEGTLKYGLPAGWTVYGGAWIAERYRAFNLGVGKNMGWLGAVSLDATRANARLPDE
                     SRHDGQSYRFLYNKSLTETGTNIQLIGYRYSTRGYFSFADTAWKKMSGYSVLTQDGVI
                     QIQPKYTDYYNLAYNKRGRVQVSISQQTGESSTLYLSGSHQSYWGTDRTDRQLNAGFN
                     SSVNDISWSLNYSLSRNAWQHETDRILSFDVSIPFSHWMRSDSTSAWRNASARYSQTL
                     EAHGQAASTAGLYGTLLGDNNLGYSIQSGYTRGGYEGSSKTGYASLNYRGGYGNASAG
                     YSHSGGYRQLYYGLSGGILAHANGLTLSQPLGDTLILVRAPGASDTRIENQTGVSTDW
                     RGYAVLPYATDYRENRVALDTNTLADNVDIENTVVSVVPTHGAVVRADYKTRVGVKVL
                     MTLMRNGKAVPFGSVVTARNGGSSIAGENGQVYLSGMPLSGQVSVKWGSQTTDQCTAD
                     YKLPKESAGQILSHVTVSCR"
     gene            complement(7430..8125)
                     /locus_tag="ACHOS5_004347"
     CDS             complement(7430..8125)
                     /locus_tag="ACHOS5_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial biogenesis chaperone SfaE/FocC"
                     /protein_id="HFP9779069.1"
                     /translation="MMKHMRIWAVLASFLVFFYIPQSYAGVALGATRVIYPEGQKQVQ
                     LAVTNNDDKSSYLIQSWIENAEGKKDARFVITPPLFSMQGKKENTLRIIDATNGQMPE
                     DRESLFWVNVKAIPAMDKAKTGENYLQFAIVSRIKLLYRPQGLVIPPEQAPGKLEFTR
                     ENGGLTLLNPTPYYLTVTDLKAGNKSLENTMVPPQGKVTVNIPGGYTGGDITYKTIND
                     YGALTEQVRGVVK"
     gene            complement(8166..8705)
                     /gene="sfaD"
                     /locus_tag="ACHOS5_004348"
     CDS             complement(8166..8705)
                     /gene="sfaD"
                     /locus_tag="ACHOS5_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial minor subunit SfaD"
                     /protein_id="HFP9779070.1"
                     /translation="MKISKMAVMLFLLSPAALAGNHWHVMLPGGNMRFQGKIIAEACS
                     LALLDRQMTVDMGQLSSNRFHAAGEYGDSVGFDIHLQDCSTVVSQRVGISFYGVSDIH
                     EPELLSVDEENDASDGIAIALFNESGELVKLNQPPENWVHLTRGDMKLHMQARYKATH
                     YPVTGGKANGQVWFSLTYL"
     gene            complement(8776..9318)
                     /gene="fimA"
                     /locus_tag="ACHOS5_004349"
     CDS             complement(8776..9318)
                     /gene="fimA"
                     /locus_tag="ACHOS5_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9779071.1"
                     /translation="MKLKFISMAVFSALTLGVATSASAAIVNGGTVHFKGEVVEAACA
                     VNMNSADQTVQLGQVRAAKLDADGKMSSPVGFDIELNDCSSTASSGASIIFSGPQGGQ
                     ADILSLQGSSAGSATNVGIKVMDYKGVAVKFDGVTASSEYTITDGTIKIPFQARYVAV
                     GGAAGAGIANADATFKVQYQ"
     gene            complement(9692..10021)
                     /locus_tag="ACHOS5_004350"
     CDS             complement(9692..10021)
                     /locus_tag="ACHOS5_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial major subunit operon
                     transcriptional regulator"
                     /protein_id="HFP9779072.1"
                     /translation="MAQHEVITRGGDAFLLKLRESALSSGSMSEEQFFLLIGISSIHS
                     DRVILAMKDYLVSGHSRKDVCEKYQMNNGYFSTTLGRLTRLNVLVARLAPYYTDSVSA
                     IAEAASL"
BASE COUNT         2734 a         2644 c         2098 g         2815 t
ORIGIN      
        1 aaaaaaccgt caggcgtccc ccataaattc agtgaagaga aacaaaggca attcattgaa
       61 tactataagg aactgaaaac aactgtgggt gatgagccga ttctttttat tgatggagtc
      121 catccgactc aggccactaa aatcagctat ggctggatac gcaaaggcca gaaaaaagca
      181 gtaaaaacaa caggcagccg cacacgcctg aatatcatgg gggcgctgaa tctgaaagca
      241 ctgacgtcac cgctaatctg cgaatataaa accattaatg aatataatgt atcccgtttt
      301 ttcaatgaga tacgaaaggt ttatcctgat tataaccaaa aaatccacgt tattctggat
      361 ggagcaggtt accaccgttc acagttagtg aaagactggg cggaagtggt aaatatcagg
      421 ctgcattacc ttccccctta cagtccaaac ctgaatccga tagaaagaat gtggaaatta
      481 atgaacgaac atgcccggaa taatcgctac ttcagcagta cgcgagagtt cagggaagca
      541 atatctgtct tttttaacca gacactgccg aatatagcgg actcactgac atcccgaatt
      601 aacgatcact ttcagatgct cacacccgca tcttgaggtt tattgagtat ataacatatg
      661 ttttttattt cattgtactt cagtcaaata aatttctgaa gcactgctag tagtgccagt
      721 tcagctttct ttttgactca ttccggcaaa gtcagtaccg ttcatctttt gtactgatgt
      781 tgccactgga aaatcggtgc gcttgtcgat catcgggaat tttgtcacaa tttctaacgg
      841 atagtgttca cattgtttct aacctgcatt tcagacacgg gcgctgctta tgtatataag
      901 atcagcatca ccaggtcttt ctgcaacact actgctttca acaagtcagg catttcacac
      961 tttatgagct gacatcatca agatgcgcca gtaatttttt attaatgact tcaaacagtt
     1021 cctgttcatc atgggtcaga taaccccaga tttctttcag atacatgctt tgtgcaagag
     1081 tgagcctctg aataaaaaac ataccttcag atgtcagact aattttttta gcacggcgat
     1141 cctccctgct atccgtcctt tttacatatc cggttttttc taaaaaatct gtaatgcggg
     1201 taatattagt tctggtaaac tgaagtttct ggcttatctc tgaaggtgac agacaatggt
     1261 tttccgctgc gaataatgtg gtgaggacca tatatgctga gtgatttatt ccatacgccc
     1321 ccagaaattc agatatatta tcctgcattc ttttatttgt gcgaatacag agctgcatca
     1381 acaaatgttc ccgaaaggga tatgcggggt ctttgttttt ttggtgtctg attatttttt
     1441 ctaatgtgtc tgtgttattc atttcattat tctccatatt actgaaacac actgtctgtg
     1501 tacaagcgcg catcagtcat ccggtaaaca tcgactttcc actacaattt attcctgcct
     1561 gatttctgta taactgagat atcccggcac agcgtacaga taaattccct cagataaaag
     1621 aacataaatg ctggctgctg acaagtctgt gaaaaaggat tatccatact gctcagtaat
     1681 cgcggataat tcatcagctc ccacacgcct gtagagccat ccctgacagc cctggacatt
     1741 catgtctctg gcaatacgga acaacgcttc attttcaaca ccttcaagta ttacgccccg
     1801 gttatgacag gcaacagtgt ctgccacagc atggagaagc ggcattgcac tcctgttttt
     1861 cagggcataa tgaaaaaact catgacttat ttttacccgt tcaaacatcc cggacagtcc
     1921 ccacattaag cctgcatttc ctgagccgaa atcatccagc cagagggtga aacgttctga
     1981 caaaaaacgt aacagggggt catcaccggg ttttaacgcc actgccggaa agtcctcact
     2041 gatctcaagc ctgatatgtg ttcccggccg ccccgggatg ccatgttttc tcagatacag
     2101 ggctaccacc cgggaaatat tgacactcag cattattttc ttctgcagac accatttatg
     2161 aagcgtttcc gcagtcttca actgactgat gagtattttg tactgaatat ccggaggcgc
     2221 acttctgaaa aaaacagatg cgcagatttt cctcccgtct tttgctgaac ggatatccga
     2281 gagcatttca aaagcaaata ttttcccggt ctgattccat acaggctcaa aaaccggtga
     2341 gaggatatat tctgttctgc ctgatttcac ctccagtata ctgttaactg cccctgacat
     2401 tactcctccg gaaatatgag cccctcccga ccagaaggtc tggctggata agcgcagttc
     2461 ttctgatttg atttttaaac cctcagattt accattatct ttatgattac tgacaacgct
     2521 cagaaccaga tatacggctc atactggttc tgtttatctt tgacaaagac catatgacag
     2581 actcgttccg tcacaacacc ggaaacaata ctattatcag ctatttttct ccacaaacag
     2641 tgatacatta aatgaaaaat tggatataaa gagcagtaat atcattaccg ccacaactgc
     2701 attctagttg tagctaaaag tcacatttat cactgactgg acagcaccgg gcgtcggact
     2761 tgcctgaccg gtcagagcat aagaggcctt cagccccaga ctcacaggag agctgcctac
     2821 ctgtcccaga gacctcggtt gtccggccgg aaccagcccc gcgttgtccg acaactgtac
     2881 acccacaccg ccggcccctg atgttgccgt atttgcgaat acagtattac cacttcctgt
     2941 tacagaacct gacagggtat agctgaccga ctgtgtctgg tcgcaacgta cagtaagagg
     3001 gactgttacc ggccctccgg gataggacgg aaggtcaact ttgacattgt tcgatgagac
     3061 cctgcatgta cctgtctgga tatagacatc attcgttgca tagaaacgcc aggtgaaatt
     3121 tctctcccct gcctcctgcc ccatggtgga aaacttatta acataaactg tggccactaa
     3181 ttctccggca tgtatcagtt tcccgaaaac gcccggctca ggataaaggt acatttttgc
     3241 cgggaaaggt ttccatacac catattgcca gtaagcttct gttggccttg tgtcttgctg
     3301 cagcggtaac gtctgactat acccagaaag cctgtttgta aagtccagcc ttccgtatgt
     3361 tttagcatct aatgaaggag aaaaaccggt tccctgtctg accctgagat agtcatagtt
     3421 ctgaccagag gcatcttcat tctgacataa caccagttga tttaaatcca gaactgtaat
     3481 ggctttatca tactgaactg ttggggaaag agtgatattg aagctactgt ctccagactg
     3541 aaaagtctgc ccggtctggt tatttctgca caacagtgca aacgatggct gggaatatgc
     3601 cattataaat gtgaataaaa cacagccctc gaggacggtg cttttcttaa agaggggata
     3661 atatttacgc ataggtatag gtgacagtaa tttgtgaatt aatacttccc gcaccagcat
     3721 tacctttgct tacggcacgg actttaagat tgaatgtcgc ctgcccattg ctcactgtaa
     3781 caacttttgt tgaaccaact ttcaaggggg tattaccatt accttgttcc tgtagctcaa
     3841 ccatgatatt agtagcatcc cctgtatttc tgtaataatt tgtattttct gcggttccac
     3901 caaaggtggc agtaacattg tttacattct gacaattcgt caatgtcaac gaaaaatttt
     3961 tccactgaga ggtgctgttg gctgctatca ggtcagaagt gtaagcgtta tccaaaacaa
     4021 cggtggcgtt tgggtttcct ccattaccaa tggtacaggt acgtggcaat accctccctg
     4081 taacagtaat cgtcgtatcc actgcctgtg ctgaaaacgc aatacagtta ataagaccgg
     4141 tggccaatat aatagctttc agtttcatct gttttcctct gtgttatatt tactgatagt
     4201 caagtacaaa attaaccacc gcatcaacat taccggcctt taccggaaga taagtggctt
     4261 tcagacgggc ataaaacgct atggtctgtg ttcccggcat taacgcaatt gttgtcggca
     4321 cattaatttt cagctcatgt ttatccttat caagaagctg aatccccaca ccccgtgctc
     4381 catttatgct gttaatctga ataagccccg cctcctgatt atctggtgtg cccgtaaatg
     4441 tcagcttcac tgcacggaca gcactgccgc aggaggtcaa cccgacatga aataccgacg
     4501 caggtgtggt atcaccagct gcggtaaact gtcggctgtc ataacttccc agactgacat
     4561 caaagtttcg ggagttaatg tcaacatcgc aggtgttatc ctgaacattg cctgtaatat
     4621 tcaccacaga acccactgca cagacatgac aggtaacgaa catactgccg gccagcatgc
     4681 aggagaatgt cactgtttta ataatatcct tcaccatttc cctgcctctt tacctgcaac
     4741 taaccgtgac atgacttaat atctgtccgg cgctttcctt cggtaattta taatctgcag
     4801 tacactggtc cgtggtctga ctcccccatt tcacactaac ctgtcctgac agaggcattc
     4861 cgctcagata aacctgacca ttctctccgg caatactgct ccccccgttt ctggccgtaa
     4921 caacagagcc aaaaggaact gctttcccgt ttctcatcag cgtcatcagc actttcacac
     4981 ccacacgggt tttatagtca gcccgcacaa cggcaccatg cgtgggcacg acgctgacca
     5041 cggtattctc tatatcaaca ttatctgcaa gggtgttggt atccagtgcc acccggtttt
     5101 cacgatagtc tgttgcataa ggcagtacag cataccccct ccagtctgta gacacccccg
     5161 tctggttttc aatgcgcgta tcagatgcac ctggtgcacg gacaagaatc agcgtatctc
     5221 ccagaggctg gctcagtgtc agaccattgg catgtgccag aataccgcca ctcagcccat
     5281 aatacagttg ccggtaaccc ccactgtgac tgtatcctgc actggcatta ccgtatcccc
     5341 ctctgtaatt cagagaggca tatcctgttt tactgctccc ctcataccct ccccgggtat
     5401 aaccactctg gatgctgtat cccagattat tgtcccccag caatgtgcca tacagccctg
     5461 ctgtactggc agcctgtccg tgagcctcca gggtctgact gtaacgggca ctggcgtttc
     5521 tccatgcaga tgtgctgtcc gaacgcatcc agtggctgaa cggaatgctg acatcaaatg
     5581 acagtatccg gtcagtttca tgttgccatg cattccggga caggctgtag ttcagggacc
     5641 aactgatgtc attcacagat gagttaaagc cagcattaag ctgacggtcc gtcctgtccg
     5701 ttccccagta actctggtgg ctgccactca gatacaacgt tgacgattcc cccgtctgct
     5761 ggctgatact cacctgcacc cttccccgct tgttgtaagc cagattgtag taatccgtat
     5821 acttcggctg tatctgtatc actccgtcct gggtaagaac actgtaacca ctcattttct
     5881 tccaggcggt atcagcaaag ctgaaatagc cccgtgtgga gtagcggtat ccgattaact
     5941 ggatatttgt ccctgtttcc gtcagtgact tgttatacag aaaacgatat gactgaccgt
     6001 catgccggct ttcatccggt aaccgggcat tcgcacgggt ggcatccagt gaaacagcac
     6061 ccagccagcc catgttttta cccaccccca gattgaaggc acggtagcgt tcagctatcc
     6121 aggctcctcc atacaccgtc cagcctgccg gcagaccata cttcagcgtt ccttcagcaa
     6181 aacggggatt atcctgctga tgtccgccac tgcgaaactc tccggcactg agagcatatt
     6241 tcagatgtcc ttcacgttgc agtacaggca ccgaagacca tggcacatta aatcgctgta
     6301 cactgccatc agcttcctgc agggttacct gtaagtcacc tccactcccg gccggataaa
     6361 tatcgtttat ctcgaacggg cctggaggta gcgtactctg gtaaatttca tacccgttct
     6421 ggcggatact aatccgggat gttccccggg aaataccgtg aattgtcggt gcataccctc
     6481 tctggctgtc cggaaccata ttgtcatcag aggccagttg aatacccctg aaatttacac
     6541 cgtcaaaaat atccccccgg gtataactgt cccccatcgt cagacgggag cgcaggggaa
     6601 ctatatcccg ctccagccag gtgttaatgt gctgccagtt attctggctg tacccctttc
     6661 cggtactgta actccaactg gtgttgtccc gaagacgcca actcccgata ttcagtcctg
     6721 ttttcagatt cagataggca taatcgctgg tgccaccata gctgtcgcgc acccgattac
     6781 cactgaagtc ataattcagc aatgccgctg taattccgtt atcccagtat tccggcgcaa
     6841 tataaccacg ggcctgatta ctcatatata tctgaggaac ggtcaggtac aatcgctgct
     6901 gcccgacatc aaaccggaat gtggcaccct gaatcatgga attcagtgat gtgcaggtgt
     6961 cagccggcag tctctccata cccggcacgg cataacggtt tacccccatc gcactcatat
     7021 gctccggact caggcagggg gcaagttgct tcccgtcctg actcatctga aactgcacgt
     7081 cacgggttga gatatacgta tcattcagat aaatatccac ccggtacacg ccgggcggaa
     7141 gctcctgccc ctgtgtaaat gcagacaggt ccgcaaccgc atccgggtca tcagacagaa
     7201 aacgcggatt aaaatagagc tcagcccggg ccggagaaac aattatcccg ataaaaaata
     7261 ccagcggcag tatattaaat ttcattctgt tattacctcc gccagcagag ccggtcagtg
     7321 atttatcaga caggagttgt ccgccatctc cggaaaacat gtcagccccc acactgagcg
     7381 aaaaaataaa atcagcagcc aaacacaacc agtaccagaa ataactgagt tacttcacca
     7441 cccccctcac ctgttcagtc agagcaccat aatcgttaat tgttttataa gtaatatctc
     7501 ctccggtgta tcctccaggg atattcacag tgacctttcc ctgtgggggt accatggtgt
     7561 tttcaagact tttatttccg gcctttaagt cagtcactgt cagataatac ggcgtcggat
     7621 taagcagagt cagccctccg ttttcacgag tgaactctaa tttacccggt gcctgttctg
     7681 gcggaataac cagtccctgt ggacggtaga gcagtttaat tctgctgaca attgcaaact
     7741 gcagataatt ctcgccggtt ttcgccttat ccatggccgg gatggccttg acattcaccc
     7801 agaacaaact ttccctgtct tccggcatct gtccgtttgt ggcatcaata attctcaggg
     7861 tattctcttt ctttccctgc atggaaaata acggaggtgt aattacaaac ctggcatcct
     7921 tttttccttc agcattttca atccatgact gaataaggta actactttta tcatcattat
     7981 ttgttaccgc cagttgtacc tgtttttgcc cttcagggta aatcacacgg gtggcaccca
     8041 gagcaacccc ggcatagctc tgcggaatat aaaaaaaaac taaaaatgat gccagaacgg
     8101 cccatatacg catgtgcttc atcatgttat taacagcctt aattaaatga cgttatatct
     8161 gccagttaca gataggtcag agaaaaccat acctgtccat ttgcctttcc cccggtgacg
     8221 ggataatgtg tggctttata cctcgcctgc atatgcagtt tcatatctcc gcgggtaaga
     8281 tgcacccagt tctccggagg ctgattaagt tttaccagct ctccgctttc attaaataaa
     8341 gcgatagcga ttccgtctga agcatcattc tcctcatcca ccgacagcaa ctccggctcg
     8401 tgaatatctg acacgccata aaaggaaatc cccacccgct gactgacgac agtgctgcag
     8461 tcctgaaggt gaatatcaaa gcccacagag tccccatact caccggcggc gtgaaatcga
     8521 ttgctgctga gttgccccat atccacagtc atctgcctgt ctaacagggc aagactgcat
     8581 gcttcagcaa ttatttttcc ctggaagcgc atgtttcctc ccggaagcat gacatgccag
     8641 tggtttccgg ccagtgcagc gggagacagc aaaaataaca ttactgccat tttcgatatt
     8701 ttcattaatc tcacccccgc atggataaaa acagccctcc ctggccgggt atatatccgt
     8761 tacactgttc tgatattact ggtactgaac tttaaaggtg gcgtcggcgt tggcaatacc
     8821 agcaccagct gcaccaccta cagcaacata gcgagcctgg aatgggattt taattgtgcc
     8881 atcagtaatg gtatattctg agctagctgt aaccccatcg aacttgactg ctacaccttt
     8941 ataatccatt acttttatcc cgacatttgt tgctgaaccc gctgagctac cctgcaagga
     9001 taaaatgtct gcctgcccac cctgggggcc agaaaaaata attgaagctc ctgatgaggc
     9061 ggttgagcta caatcattta attcaatatc aaaaccaaca ggtgaactca tttttccgtc
     9121 agcatctaat tttgccgctc gaacttgccc caattgaact gtctggtctg ctgagttcat
     9181 attcacagcg catgcagcct caacaacctc tcctttaaag tgaacagtac cgccattgac
     9241 gattgcggca gacgcacttg tcgcaacacc cagggtcaga gctgaaaata cagccatgga
     9301 gatgaatttt aacttcatat ttaattcctg tttttaattg aactacgcac accgtatctg
     9361 tcatcacaac agacacaatc aatatcccga acgacgggaa tcttttttca ggagaacttc
     9421 ataaatgcaa tgtgagaaat acactcaggc agcatttatg acattatctc ccgataatat
     9481 tttccggata accgacagga atcttctcta tagataagat tccctggatg ataagctatg
     9541 ggttagtgtt cctgtctcca caccgaatgc tttagcaaaa gcagagaagc tgaaagcata
     9601 cctcgttgat aacacccgta ctctcatttg actacaggct tatatttctc gcgtattatg
     9661 tattgccccg gcgtttttta ttgtccgtat tttacaggga agcagcttca gctatagctg
     9721 atacactatc agtataataa ggagcaagtc tggcaacgag gacattcaac ctggtaaggc
     9781 gcccaagcgt tgtactgaaa tatccattat tcatctggta tttctcgcag acatctttac
     9841 gggaatgacc actgaccaga taatccttca tagcgagaat aactctgtca ctgtgaatag
     9901 aggaaatacc tatcagtaaa aaaaattgtt cttcagacat ggagccggat gacagcgcgc
     9961 tctcacgtaa ttttagtaaa aaagcatccc ctccccgggt aataacttca tgctgtgcca
    10021 ttcgatactc cccaccccgc cacctacagg gctgccttaa tattcatccc ccatacagga
    10081 gggccttgct ccttaagata tatttagttt tttatgttgt aaatatgtat tttgacgatc
    10141 ttttgatctg taaagccgtt tatttttagg gttatatctt gcagtggatt agcgccttag
    10201 tgattaatgc tgtataagaa tgaatattca gtataaaaga tcgttaaaat cgttttttgc
    10261 aacgttcctt accattgaaa aaactataaa a
//
LOCUS       DAZKHK010000155        10240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKHK010000155 DAZKHK010000000
VERSION     DAZKHK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.156"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            243..1013
                     /locus_tag="ACHOS5_004351"
     CDS             243..1013
                     /locus_tag="ACHOS5_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP9779073.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1004..1477
                     /locus_tag="ACHOS5_004352"
     CDS             1004..1477
                     /locus_tag="ACHOS5_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP9779074.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1585..2124
                     /gene="dnaT"
                     /locus_tag="ACHOS5_004353"
     CDS             1585..2124
                     /gene="dnaT"
                     /locus_tag="ACHOS5_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP9779075.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     ITPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2127..2864
                     /gene="dnaC"
                     /locus_tag="ACHOS5_004354"
     CDS             2127..2864
                     /gene="dnaC"
                     /locus_tag="ACHOS5_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP9779076.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2913..3407
                     /gene="yjjA"
                     /locus_tag="ACHOS5_004355"
     CDS             2913..3407
                     /gene="yjjA"
                     /locus_tag="ACHOS5_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP9779077.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3661..5952
                     /gene="opgB"
                     /locus_tag="ACHOS5_004356"
     CDS             3661..5952
                     /gene="opgB"
                     /locus_tag="ACHOS5_004356"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP9779078.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFTAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEVFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQALVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6092..7114)
                     /locus_tag="ACHOS5_004357"
     CDS             complement(6092..7114)
                     /locus_tag="ACHOS5_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFP9779079.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDLEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7253..8158
                     /locus_tag="ACHOS5_004358"
     CDS             7253..8158
                     /locus_tag="ACHOS5_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9779080.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8329..9981)
                     /locus_tag="ACHOS5_004359"
     CDS             complement(8329..9981)
                     /locus_tag="ACHOS5_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9779081.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQISAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2698 a         2620 c         2402 g         2520 t
ORIGIN      
        1 cagctatcta ttatcctgac tcgtaaaggc caaataacaa attcattata gtgataatat
       61 tcatgcgata gtttgtttac ggcgagagat aattcgcttt ttatcaccga gcatctcccg
      121 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca
      181 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      241 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
      301 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      361 cgttgggaat ggacagcgtc gaaagttcta tctcttcgaa cgccatagtg ctgacaacca
      421 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      481 atgtggtgac tgaagtccag cacattgtaa ttctggcgga gcatcatctg ctggattaca
      541 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
      601 ccttaatggt tggcctttcc tgcgcctgtt tctgtaaact caataaaggt ggctgggatg
      661 gtgccgtcat caccttcttt gccagtacgg ccgcgatgta tatccgccag ttgctggcac
      721 aacgtcatct tcatccacag atcaactttt gcctcaccgc tttcgccgcc accaccattt
      781 ccggattgct tttgcaactt cccactttca gcaatacccc caccattgcg atggccgcca
      841 gcgttctgct gctggtgccg ggctttccgt tgataaatgc cgtcgccgat atgtttaaag
      901 gccacatcaa taccggactg gcacgctggg cgatcgccag tctgctgaca ctggctacct
      961 gtgtcggcgt agtaatggca ctgacgattt gggggctacg cggatgggtg tgatcgaatt
     1021 tctgttagcg ttggcgcagg atatgatcct cgccgccatt cctgcggtcg gctttgcgat
     1081 ggtgttcaac gttcccgttc gagcgttacg ctggtgtgcg ctgcttggtg cgataggtca
     1141 tggttcccga atgatcttaa tgaccagcgg gctgaatatt gaatggtcaa cctttatggc
     1201 ctctatgctg gtcggcacca ttggtattca atggtcgcgc tggtatctgg cgcatccgaa
     1261 agtgtttacc gtggcggctg ttatccctat gttccctggc atatcagctt ataccgcaat
     1321 gatttcggcg gtaaaaatca gccagctagg ttacagcgaa ccgttgatga ttaccctgtt
     1381 aaccaacttt cttacagctt catcgattgt tggtgcgtta tccatcggtc tttccattcc
     1441 tggattatgg ttgtaccgca agcgccctcg cgtataaaat tgcctcactt aaagcacgcg
     1501 gatgagagac agcctcctct cctccgtgtg ttactataaa agttatctcc cttctcgttc
     1561 atcgttccat atttgagaaa cagtatgtct tccagagttt tgaccccgga cgtcgttggt
     1621 attgacgccc tggtacacga tcaccaaacc gttctggcaa aagctgaagg cggtgtggtt
     1681 gccgtatttg ctaacaatgc cccggcgttt tatgccatca ctcctgcacg cctggctgaa
     1741 ctgctggcgc tggaagaaaa gctggcgcgt ccgggaagtg acgtggccct ggacgatcaa
     1801 ctctatcagg aaccgcaaac cgctcccgtt gccgtaccca tggggaaatt tgccatgtat
     1861 ccggactggc aacccgatgc cgactttatc cgcctggcgg cgctatgggg cgtggcacta
     1921 agagaaccgg tgaccgccga agaactggcg tcattcattg cctactggca ggcggaaggc
     1981 aaagtctttc accatgtgca gtggcaacaa aaactggcgc gcagcctgca aatcggtcgt
     2041 gccagcaacg gcggactgcc gaaacgagat gtgaatacgg tcagtgaacc tgacagccaa
     2101 attccaccag gattcagagg gtaacgatga aaaacgttgg cgacctgatg caacgcctgc
     2161 aaaaaatgat gcctgcccat atcaaacctg ctttcaaaac gggtgaagaa ctactggcct
     2221 ggcagaaaga acaaggggcg atccgctccg ccgctctcga acgtgaaaat cgggcgatga
     2281 aaatgcagcg cacctttaac cgctccggta ttcgtccact gcatcagaac tgctcctttg
     2341 agaactatcg cgttgagtgt gaagggcaaa tgaatgcgtt aagcaaagcg cgccagtatg
     2401 tcgaagagtt tgacggcaac atcgccagct tcatcttttc tggtaagcca ggaaccggca
     2461 aaaaccatct ggcggcggca atctgcaacg agctgctgct acgcggtaaa tccgtattga
     2521 tcatcaccgt ggccgatatt atgtcggcga tgaaagatac cttcaggaat agcggtacca
     2581 gcgaagaaca actgcttaac gatctgagta acgttgatct gctggtgatc gatgagatcg
     2641 gcgtgcagac cgaatcgaaa tacgaaaaag tgatcattaa ccagatcgtc gatcgccgct
     2701 cttcttccaa acgcccaacc gggatgctga ccaacagtaa tatggaagag atgaccaagt
     2761 tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa cagtttgtgg gtgatcttta
     2821 actgggatag ctaccgtagc cgtgtaacag gtaaagagta ttaatccccg tttctggcgg
     2881 tatactgcga cacaattcaa ccttgagtaa tgatgatgaa aactattaaa catcttctgt
     2941 gctgtgccat tgccgccagc gcattaattt ccaccggggt gcatgctgcg tcctggaaag
     3001 atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa caacacgaca caggaaggcg
     3061 gttggtcgct cgcgtcatta actaacttgc ttagcagcgg gaaccaggcc ttaagcgcag
     3121 ataacatgaa caacgccgca ggcattctgc aatactgcgc gaagcaaaag ctggcttcgg
     3181 taaccgatgc cgaaaacatc aagaatcagg tactggaaaa actgggcctg aacagtgaag
     3241 agcaaaaaga agacaccaac tatctggatg gcattcaggg tttgctgaaa accaaagatg
     3301 gtcagcaact caatctggat aatattggaa cgactccgct ggcagaaaag gtgaaaacca
     3361 aagcctgcga tctggtgtta aagcaggggc tgaacttcat ttcctgaacc gcgaaacgcc
     3421 gcgttttagc tgttacccgg ctgacgcggc tctctcctgc ccttttccct gctcctggac
     3481 ggttttaccc ctgttacacg ggaagcgacc gcgatcaaaa aagtctgttt ataaactaat
     3541 tcctaacaac atcgcatttt tttgacacta gaattgcagc aatatggcga cgccattaca
     3601 ttgtgcttct caaaaagctt aaacgttggc taaacggccc ggtttagtga ggatcgcccg
     3661 ttgtcagaac tactctcttt cgcccttttt ctcgcctctg tgctgattta cgcatggaaa
     3721 gcgggacgta acacctggtg gtttgcggcc acattaacgg tactggggct atttgtcgtt
     3781 ttaaatatca ccctgtttgc cagcgactat tttactggcg atggtattaa cgacgcggtt
     3841 ctctatacct taaccaacag cctgaccggt gctggtgtca gcaaatacat cctgcctggt
     3901 atcggcattg tgctggggct ggcggcggtg ttcggtgcgc tgggctggat cctgcgccgt
     3961 cgtcgccatc atccgcacca ttttggttac agcctgctgg cactcttact ggcgctgggt
     4021 tcagtggatg ccagcccggc atttcgtcag ataacggaac tggtgaaatc ccagtcacgc
     4081 gacggcgacc cggactttac ggcttattat aaagagccgt cgaaaactat ccctgacccg
     4141 aaactcaacc tggtttatat ctacggcgaa agtctcgagc ggacctattt tgataacgag
     4201 gttttcccgg atctcacgcc tgaactgggc gcgttgaaaa atgaaggcct ggatttcagc
     4261 cacacgcagc agctgccagg aacggattac acgattgcgg gcatggtggc ttctcagtgc
     4321 ggcataccgc tgtttgcccc atttgaaggc aacgcctccg cctctgtctc cagcttcttc
     4381 ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg gttatcagaa ctatttcgtg
     4441 cagggcgcga atctgcgttt tgccggtaaa gatgtgttcc tgaagtcgca tggcttcgac
     4501 cacttatacg gctcagaaga gctgaaaagt gtggtggccg acccgcacta tcgcaacgac
     4561 tggggattct acgacgatac cgttctcgat gaagcgtgga aaaaatttga agagctttcc
     4621 cgctcaggtc agcgattctc actgtttacc ctgacagtcg atacccatca cccggatggt
     4681 tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg acggtaagcc gaatcagtca
     4741 ttcagcgcgg taagttgcag ccaggagaat attgcgacgt ttatcaacaa aatcaaagcg
     4801 tcaccgtggt ttaaagatac cgtcatcgtc gtctcttctg accatttagc gatgaacaat
     4861 acggcgtgga aataccttaa taagcaggat cgcaataacc tgttttttgt catccgtggc
     4921 gacaagccgc agcaagagac gctggcagtg aagcgtaata cgatggataa cggcgcgacg
     4981 gtgctggaca ttctcggtgg cgataactat ctcggacttg gccgtagcag tttatccggg
     5041 cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa cattggcgtg gaagccggat
     5101 atcatccgcc tgtggaaatt ccctaaagag atgaaagagt tcaccatcga ccagcagaaa
     5161 aacatgattg ccttctcagg tagccatttc cgtttgccgc tgctgttgcg ggtttcagac
     5221 aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac cgctgcgttt ccagctggca
     5281 gatttcgctc cacgcgacaa tttcgtctgg gttgaccgtt gctacaagat ggcacaactc
     5341 tgggctccgg aactggcact ctccaccgac tggtgtgtct cgcaagggca gcttggcggt
     5401 cagcaaattg ttcagcatgt tgacaaagca atatggaagg gcaaaacggc atttaaagat
     5461 acggtcatcg acatggcgcg ttacaaaggc aatgtcgata cgctaaagat tgttgataac
     5521 gatattcgct acaaagccga cagtttcatc tttaacgtcg ctggtgcgcc agaagaggtg
     5581 aaacagttta gcgggatttc tcgtccagag tcgtggggcc gctggtccaa cgcacagctg
     5641 ggcgatgaag taaaaatcga gtacaagcat ccgctgccga agaaatttga cctggtgatt
     5701 accgccaaag catacggcaa taacgccagc cgtcctattc cggtacgcgt aggcaatgaa
     5761 gaacaagccc ttgtgctggg caatgaagtg accaccacca cactgcattt cgataaccca
     5821 accgatgccg acacactggt aattgtgcca ccggaacctg tctcaaccaa cgaagggaat
     5881 atcctcgggc actcgccgcg taagctcggg atcggcatgg tggaaattaa agtggtagaa
     5941 cgtgaagggt aattcctgac tgatgtgtag gtcgcataag gtgcgaccta cacaccttct
     6001 ccggcctaca aaacattatt gagaaacaga aaaaaaacag cacccaacac ttccccggac
     6061 gctgcatggc aacttgaata atctcgcctt atcagaaagt aattacgccc ttaatcaact
     6121 cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac ggatagcgat
     6181 gggttaacat catgtcggca gtgattttcc cttccgccat caatcgaccg actttagcga
     6241 aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta tggaattcca
     6301 gatcggagaa ctgcaactca cctttaaaca ggccgacaaa taccaccgtg ccgccgtgac
     6361 gaatcagatt tacggtgtta ttcatcgcat gttgattacc tgtcgcgtca atcactttct
     6421 gcgccagcga accaccaaac tgcgcccgca gttgcgcgtc aaaatcttcg gctgacgggt
     6481 ccagaacagg taattccaga cgcgttgcca catgttcacg gcgcgccgga ctggtatccg
     6541 ccaccaccac ctgcgcgcca tcggctttag cgattgccgc cgcgcccaga ccgattggcc
     6601 ccgccccgac caccagcacc tgctcgctgg gtgcaatggc tgcacgacgc accgcatgag
     6661 cgctaatagc gaaaggttca atcaatgctg ccgcctgcgg gtcaataccg tctgcgggca
     6721 aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga tgcacgccaa
     6781 tgactgaaat tttttcacag caattggtac gaccgctttt acacgccggg cattgctgac
     6841 aagaaatata cggaattacc gcaacttgct gtccgatatt aaaattgtga atgttttttc
     6901 ctgattcaat aatctcccca catatttcat ggcctaaaac gcgtggataa ctgaaaaatg
     6961 gctgattgcc cgcccaggca tgaatatctg taccgcaaat cccgacagtt ttaattttaa
     7021 ttaatgcttc attatcgcct ggcaccggtc tttcccggac ttcccatact aaattcttta
     7081 tttcctgaca aattaatact ttcattgtgg acatgattca ccgctccttt tattttatga
     7141 ctttcagata tcgtcgaatt tataagagcg cctgaattta cccataaaat atgcggatga
     7201 tcgcgcataa aaatgtttta aatcggcttt taatgccgga ctggagggaa gaatgagtcg
     7261 ttcgcagaat ttacgtcata gcgtcataaa tcaggtgatt gaagatatgg cgaggggcaa
     7321 tattccttct ccgctgcctt cacaaagcgg actggcagaa atgtataaca tcagtcgcac
     7381 tacggtacgc cacatactcc agcatttaag cgcatgtggc gtgctcaccc tggtggggaa
     7441 aaattatgtt atcgcccgca aacccgaaca gaacgatgga tttgattgca tcacggcctc
     7501 gctgaaagag caaaaccgtc ttttcgagca ggcatttttc accatgatta acctgcgcca
     7561 gttacgcgcc ggagaaagct tttccgaact tcagctggcg cgagcggcaa gcgtcagtcc
     7621 ggtagtggta cgagaatatc ttttgaaatt cggtcgttat aatcttattc aaagcgaaaa
     7681 gcgcggccaa tggagcatga agcagttcga tcaatcctac gccgagcagc tgtttgagct
     7741 acgcgaaatg ctcgaaacac attccctgca acactttctc aaccttcccg atgatgaccc
     7801 tcgctggctg caagcaaaaa ccatgctcga acgccaccgg acgctgcgcg acagcattgg
     7861 cagcaacttt cgaatgtttt ctcaattaga ccgtgatttt catgccatgc tgttatccgc
     7921 tgctgacaac atctttttta atcaatcact tgaaattata tcggtgatct ttcattttca
     7981 ctatcaatgg gatgaaagcg atctcaaaca gcgcaatatc attgctgttg atgaacatat
     8041 gaccattctc agcgccctga tttgccgtag cgatttagat gcaataacgg cgttgcgaaa
     8101 ccatctggat accgccaaac aatcaatgat tcgttccatc aggcaacatc accgataaat
     8161 taagtacaga ttaaaaacat tgattcttgg tttatatcac tatgttgtat taatgcagta
     8221 ttaatccagg cgattgttct gcccataagg aaatacatag ggaataaacg atgctggaag
     8281 tattactctc agccatcatt taattcccta ttactggcag cgataaaatt atcggctatc
     8341 catgattaac gtttccagca catctaattc actgactatt tttgccagtg tttcatcact
     8401 gacttcttgc cttgcgcgca tctggtaaat agccgcgcgt ttggcattta acgccgccag
     8461 attaaatgtt ctttccagct tcgcacgctg catatgcagg gaattatcat taggcgacat
     8521 atcctcttca ctatgtcgca tataaccgat aactctggcc cccgtttcca taatcaactc
     8581 gttatcgagg ttttctgttc tggcgcgcag caaacgttgc tcggtctttt gcacactcag
     8641 tatggccatt ttagccataa acagatcggc agcttcctct tctttttgtc ggttatcgct
     8701 gaaaatcccc acatttttcc ccagtaataa cggcagaata ataatgccgc aaaccagcga
     8761 aaagaggatc acacctgtag cgagaaatac cagcaagtaa cgcccgggaa aaggtgtgcc
     8821 atccggcagc aataacggga tagaaagcac ccccgcaagc gtgatagctc cgcgcacccc
     8881 cgcaaaggtc gccagcagca tatgacgcgt tttatatcca gcaaacagca tcggatgttt
     8941 gccgggtcgt ttacggctat acagacgcat cagccaaagc cagcccaggc gcacaaccat
     9001 taacgccagg tagatccaaa tgacatcaag gagcagagat ccggtgtcca ccagtggatc
     9061 gttttctgca cggatcagtg attccgaaat aatgcccggc agttgtaagc ctaaaatcag
     9121 aaacaccatg ccgttaaaga catattcaag catcgaccag ctacttttag ctttaattct
     9181 caggcgaata ggcgcgtggt ggactacgcc aatgcgactg gttgttaacc ccgcggcgac
     9241 agcggcaaga atgccagata agccaaaatg ttccgcaacc agataaacaa taaacggcaa
     9301 cagaagcatc aatacaatct gaatggaagg ttcatcgtga gcatatcggc tgatcaacag
     9361 cagcccttta ccatataacc aggtaacagc gatccccacg gcaataccgc ccaacgcaac
     9421 gacaaaaaac gcaatggttg cgccatgaac gctgaattcc atcgttccca tcgtcactgc
     9481 aacagcaaat ttcaacgcca ccaggccgga agcatcattc atcaacgctt cgccttcgag
     9541 cacttcctgg atgtttttgc tgatacgccc tttcccgaca atccccagca acgccacggc
     9601 atcggtaggc gacaacacgg cggctaatgc gaaagcagcg atgagcggca cattcggtaa
     9661 gagtaaatga atcacatagc cgacaccaaa aatagtgatt agtaccaaca caagtgccag
     9721 gctgataatt tcccggctgt gcttgatcaa ttctttaggt tgtatttttg aactttcagc
     9781 aaacaacagg ggtggtataa acaataagag aaaaagttca ggattgaatt ccacatgcag
     9841 accaaatata ggctgggcca gtactgcacc agcggaaatc tggattaaag gaagtggtat
     9901 ctggacagga gacagacgtg taagcacgct ggttaatgat actaccaacg taagaattaa
     9961 aattgttaaa agtatttcca tataataaac aggcttcctc attcataaat aatggatatt
    10021 attaatttaa cttcataaaa agcgctctgg ttagcactta acgttaatca ttaatcactg
    10081 cgttcactca catgatcaga aggaacccag taaaataagt tttcaagttg ataacaaatt
    10141 caccatgtga atatttactg cctttccccg ttgtaacgaa tcatccgtta ctctctgatt
    10201 tccttgccga acaagttatt tagtgaatga cggctttctt
//
LOCUS       DAZKHK010000156         9894 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKHK010000156 DAZKHK010000000
VERSION     DAZKHK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.157"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..580
                     /locus_tag="ACHOS5_004360"
     CDS             32..580
                     /locus_tag="ACHOS5_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9779082.1"
                     /translation="MNVNKKKLAEIFNVDIRTIDRWQTQGLPQISGGGKGVEVIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASESDLQPGTIDYERYRLTKAQADAQELKNA
                     REEGLVLETELFTYIFQRVAQNISGILVRVPQTLQRKYPEISPAHLDAMKTEIAKASD
                     VASEAGENVRRWIDDFRRTEGG"
     gene            552..2480
                     /locus_tag="ACHOS5_004361"
     CDS             552..2480
                     /locus_tag="ACHOS5_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9779083.1"
                     /translation="MISDELRAANSAGAIATGLLALKIPVPLTTVQWADRHYYLPKES
                     SYTPGRWETLPFQVAIMNSMGNDRIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEPTIRDVSVLLDLAPWFGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2464..2670
                     /locus_tag="ACHOS5_004362"
     CDS             2464..2670
                     /locus_tag="ACHOS5_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9779084.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2667..4259
                     /locus_tag="ACHOS5_004363"
     CDS             2667..4259
                     /locus_tag="ACHOS5_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9779085.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRSRFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETRPRET"
     gene            4249..5754
                     /locus_tag="ACHOS5_004364"
     CDS             4249..5754
                     /locus_tag="ACHOS5_004364"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9779086.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALSAEVCQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            5791..6138
                     /locus_tag="ACHOS5_004365"
     CDS             5791..6138
                     /locus_tag="ACHOS5_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9779087.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            6196..7224
                     /locus_tag="ACHOS5_004366"
     CDS             6196..7224
                     /locus_tag="ACHOS5_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9779088.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPTYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7276..7659
                     /gene="gpFI"
                     /locus_tag="ACHOS5_004367"
     CDS             7276..7659
                     /gene="gpFI"
                     /locus_tag="ACHOS5_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9779089.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEPLNNTDAPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSSLAMAMIQHGTAEYA"
     gene            7652..8005
                     /locus_tag="ACHOS5_004368"
     CDS             7652..8005
                     /locus_tag="ACHOS5_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9779090.1"
                     /translation="MRDVQNAFDAALVGVDSTIVEVMGIRVQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8021..8596
                     /locus_tag="ACHOS5_004369"
     CDS             8021..8596
                     /locus_tag="ACHOS5_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779091.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTTINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            8593..8988
                     /locus_tag="ACHOS5_004370"
     CDS             8593..8988
                     /locus_tag="ACHOS5_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9779092.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAAIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            8996..9745
                     /locus_tag="ACHOS5_004371"
     CDS             8996..9745
                     /locus_tag="ACHOS5_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779093.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            9762..>9894
                     /locus_tag="ACHOS5_004372"
     CDS             9762..>9894
                     /locus_tag="ACHOS5_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9779094.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAEQQGTTKHD
                     "
BASE COUNT         2309 a         2360 c         3072 g         2153 t
ORIGIN      
        1 tcgtaactga tttttgataa ggaattgatg aatgaatgtt aataaaaaga agcttgctga
       61 aatttttaat gtcgatatac gcactattga tcgctggcaa actcaggggc tccctcagat
      121 atctggtgga ggaaaaggtg ttgaggtcat ctttgattct gcgaaggcga tcgaatggta
      181 tgctcagcgc gaagctgata ttgaaaatga aaaactccgt aaagaggtcg aggatttgag
      241 ggctgccagc gaatcagacc ttcagcccgg caccattgat tacgaacgtt accgactgac
      301 gaaggcacag gcagatgcac aggagctgaa aaatgctcgt gaggaaggcc ttgtcctcga
      361 gacggagtta tttacctaca tctttcagcg agtggcacag aatatatctg ggatccttgt
      421 ccgtgtccct cagacactgc agcgtaaata ccctgagata tcacccgcac atcttgatgc
      481 tatgaaaact gaaatcgcga aagcatccga tgtggcttct gaagccggtg agaatgtgcg
      541 caggtggatt gatgatttca gacgaactga gggcggctaa ttctgcagga gcgatagcaa
      601 ccggccttct tgcgctaaaa attcctgtcc ccctgacgac agttcagtgg gcagatcgac
      661 actattacct tccgaaagag tcatcttaca ccccggggcg gtgggaaaca ctgccatttc
      721 aggttgccat catgaacagc atggggaatg accggatccg cacggttaat ctgattaaat
      781 ctgcccgtgt tggttataca aagatgttgc tgggagtgga ggcttatttt attgagcata
      841 aatcacgcaa cagccttctt ttccagccta cggattctgc tgctgaagat tttatgaaat
      901 ctcatgtgga acccacgatc agggatgtgt cggttttact cgatcttgca ccgtggtttg
      961 ggcgtaaaca tcgtgataat accctcacgc tgaaacgctt ttcatcgggt gtgggtttct
     1021 ggtgcctggg tggtgcggca gcaaaaaact accgtgaaaa atccgtggat gtggtctgct
     1081 atgacgaact ttcctcgttc gagccggatg ttgaaaaaga gggctcgcca actctgctgg
     1141 gggataagcg tattgagggc tcggtatggc caaaatccat tcgcggctcg acgcctaaaa
     1201 taaaaggcac ctgccagatc gaaaaagcgg ccaacgagtc ggcgcatttc atgcgttttt
     1261 atgtgccctg cccgcactgt ggggaggagc agtatctgaa atttggcgat gaatccacgc
     1321 cttttgggct taaatgggag aaggacagtc ccgaaagtgt tttctacctc tgtgaacatc
     1381 atggctgcgt gatccatcag tctgagcttg accagagcaa cgggcggtgg atctgtgaaa
     1441 acacggggat gtggacccgt gacggtctga cgtttttcag cgctgcgggt aatgaaattc
     1501 cgccgccgcg ctccatcaca ttccatatct ggacggcgta cagtccgttc accacctggg
     1561 tacagattgt ctatgactgg ctggatgcac tgaaagatcc caacggcctg aaaacttttg
     1621 tgaacaccac gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg
     1681 tgctgatgga taaggttgtg cgttacacgg cggcggtacc tgcccgggtg gtttatctga
     1741 cggcgggcat tgactcgcag cgtaaccgtt ttgagatgta tgtctgggga tgggctccgg
     1801 gagaggaagc ctttctggtg gataaaatca tcattatggg gcgtcctgat gaggaagaga
     1861 ctctgttacg tgtggatgcg gcgatcaaca aaaaataccg ccatgcagac ggaaccgaaa
     1921 tgaccatttc ccgtgtctgc tgggacaccg gggggatcga tggcgaaatc gtttatcaga
     1981 ggtcaaaaaa acacggtgtt ttccgggtgc tgccggtaaa aggcgcatct gtctatggca
     2041 agccggtgat caccatgcca aaaacccgca atcagcgggg cgtgtatctg tgtgaagtgg
     2101 ggacggacac cgcaaaagaa attctctatg cccgtatgaa agccgatccc acgcctgcgg
     2161 atgaagccac gtcgtatgcc atccgttttc ctgatgatcc ggagattttt tcgcagacag
     2221 aggcgcagca actggtcgcg gaagagcttg tggagaagtg ggaaaaagga aagatgcgtc
     2281 tgctgtggga taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctacg
     2341 cggcattacg tgtgtccgtg caacgctggc agcttgatct ggctgtactg gcaaaatccc
     2401 gggaagaaga gacgacccgg ccaaccctga aagaactggc agcgaagctg tccggaggag
     2461 tgaatggtta cagtcgctga actgcaggcg ctgcgtcagg cgcgccttga tttattaacc
     2521 ggtaaacggg tggtgtctgt ccagaaagat ggtcgcagaa ttgaatatac ggcggcttct
     2581 ctggatgagc ttaaccgtgc gatcaatgat gcggagtcgg tactggggac aacccgacgt
     2641 cgccgtcgtc cgctgggagt gaggttatga aacgaacgcc tgtcctgatt gatgtgaacg
     2701 gcgttccgct tcgggagagc ctcagctacc acgggggcgg tgcaggattt ggtgggcaaa
     2761 tggcagagtg gttgccaccc tcgcagagtg ccgatgcggc cctgctgccc gcgttgcgtc
     2821 tggggaatgc ccgtgcagat gatctggtac gcaataacgg gatagcggct aatgcggtgg
     2881 ccctgcataa ggatcatatt gtcgggcata tgtttctgat cagctaccgt ccgaactggc
     2941 gctggctggg gatgcgggag actgcggcaa aaagctttgt cgatgaggtg gaggcggcct
     3001 ggtcggaata cgccgaaggg atgtttggcg agatcgacgt ggaaggaaaa cgcacgttca
     3061 cggaatttat ccgtgaaggc gtgggcgttc atgcgtttaa cggcgaaatc tttgtgcagc
     3121 cggtctggga tacggaaacc acgcagttat tccgcacgcg ttttaaagcc gtgagtccga
     3181 aacgggtgga cacgccagga cacggtatcg ggaatcgttt tctgcgggcc ggggtggagg
     3241 tcgatcgata tgggcgtgcc gttgcgtacc atatctgtga ggatgatttt cctcgctccg
     3301 ggagtggacg atgggaacgg atcccgcgtg aacttcccac cgggcgtccg gccatgctgc
     3361 atattttcga gccggtggag gacgggcaga cccgtggggc caatcagttt tacagcgtca
     3421 tggaacggct gaagatgctc gattccctgc aggcaacaca gcttcagtca gccattgtta
     3481 aagccatgta tgcagcgacg attgaaagtg aacttgatac cgaaaaggcc tttgaatata
     3541 tcgcgggggc accgcagggt cagcaggata atccgctcat taatattctg gagaagttct
     3601 ccagttggta tgacacgaat cacgtgacgc tgggcggtgt caaaattccg caccttttcc
     3661 ccggtgatga tctgaaactg cagaccgcgc aggattcaga caatggattt tctgcgcttg
     3721 aacaggcgct gctgcggtat atcgccgccg gtcttggcgt ttcctacgaa cagttgtccc
     3781 gtgattactc gaaggtcagt tactcaagtg cccgcgcctc cgccaatgag tcgtggcgct
     3841 attttatggg gcggcgaaaa tttattgcgg cccggctggc cacgcagatg ttttcctgct
     3901 ggctggaaga ggcacttctt cgggggatta tccgtccgcc acggtcacgt tttgattttt
     3961 atcaggcgcg atcagcctgg tcacgggcag agtggattgg tgccggaaga atggccattg
     4021 acgggctcaa ggaggtccag gaatcagtga tgcgcattga ggccggactg agcacgtatg
     4081 agaaagagct ggcgctgatg ggtgaggatt atcaggacat tttccgccag caggtcaggg
     4141 aatccgcaga gcgggaaaaa gccggactct cacgtccggt gtggatagca caggcgtatc
     4201 agcagcagat agcggagagc cgcaggccgg aagaggagac aagaccacgt gagacgtaat
     4261 ctttcacaca ttattgccgc agcattcaat gaaccgctgc ttctggagcc cgcctatgcg
     4321 cgggttttct tttgcgcgct cgggcgcgag atgggggcag caagtctttc ggtaccacaa
     4381 cagcaggtgc agcttgatgc tcccggaatg ctggctgaaa cggacgagta catggccgga
     4441 ggtaaacgac cggcccgtgt ttaccgggtg gtgaacggta ttgctgtact gccggtgacc
     4501 ggcacgctgg tgcaccggct gggtggcatg cggccatttt ccggaatgac aggctatgac
     4561 ggcattgttg cctgtcttca gcaggcaatg gcggacacct ctgtccgggg cgtactgctg
     4621 gatattgaca gtccgggcgg gcaggccgcc ggtgcgtttg actgcgctga catgatttac
     4681 cgcctccggc agcagaagcc ggtctgggca ctgtgtaatg acacggcctg ttctgcggcc
     4741 atgctgctgg cgtcggcctg ctcccgacgg ctggttaccc agacatcccg tatcggctcc
     4801 attggcgtga tgatgggcca tgtcagctat gccggtcatc tggcgcaggc cggggtggat
     4861 atcacgctga tttatgccgg ggcgcataag gtggatggca atcagtttga agccttatcg
     4921 gcagaggtgt gtcaggacat gcagcagcgc attgatgcgg cgcaccggat gtttgccgaa
     4981 aaagtggcga tgtatacggg gctgtctgtg gaagctgtca cggggacaga ggctgccgtt
     5041 tttgaaggtc agtccggtat tgaggccggg ctggcggatg agttaatcaa tgcgtcggat
     5101 gccatcagcg tgatggctgc ggcgctgaac acacatgata caggaggcac tatgccgcaa
     5161 ttaactgcaa cggaagccgc cgcgcaggag aaccagcgag tgatggggat cctgacgtgt
     5221 caggaagcga aaggacgtga acagcttgcc acgatgctgg caggacaaca gggcatgagc
     5281 gttgaacagg cccgggcgat tctggctgcg gcagcaccac agcagccggt ggcatccgcg
     5341 cagagtgaag ccgatcgcat tatggcgtgt gaagaagcta aaggtcgtga acaactggcg
     5401 gcaacgctgg cggcgatgcc ggagatgacg gtggaaaaag cccgcccgat cctggcggct
     5461 tcaccgcagg cggatgccgg accctcactc cgtgatcaga ttatggcttt ggatgaggca
     5521 aaaggggctg aggcgcaggc tgaaaaactg gcggcgtttc ccggaatgac ggtggaggct
     5581 gcccgcgaca ttctgtcctc atcgccggat aaagcagaac cggtttctgc atccacaacc
     5641 gccatgtttg aacgcatcat ggcgaaccat tcaccggcag ccgtgcaggg tggcgtgtca
     5701 cagacgtcgg cagacggtga tgcggacgtg aaaatgctca tggccatgcc atgaagtcag
     5761 tgctgaccat caatatgagg tttttacaat atggtaacga aaaccatcac tgaacaacgt
     5821 gcagaagtac gtatttttgc cggtaatgat ccggctcata ccgccacagg cagcagcggg
     5881 atttctcagg caacaccggc actgacgccc ctgatgctgg atgaagccag cgggaaactg
     5941 gtggtctggg acggacagaa agccggtagt gcggctggca tactggtact gccgcttgaa
     6001 ggcacagaga cggtgctgac gtattacaag tcgggaacct ttgcgacgga ggcaatccgc
     6061 tggcctgaaa gtgtggatga acacaaaaag gccaacgcct ttgtcggcac agccctgagt
     6121 cacgcggcgc tgccgtaaca cgttatcagg ccaccgcgtt ggcctgactg atttctgaat
     6181 gaaaggaact gatttatggg attgtttacg acccgccagt tactcggtta taccgaacaa
     6241 aaagtgaaat ttcgtgcgct gtttctggaa ctgtttttcc gccgtacggt gaatttccac
     6301 accgaagagg tgatgctgga caaaattacc ggaaaaacgc cggtggcagc ctatgtctcc
     6361 ccggttgttg aaggaaaagt gctgcgtcat cgtggtggtg aaacccgcgt gttacgtccg
     6421 ggctacgtca agccgaaaca cgaatttaat taccagcagg cggtggagcg ccttcccggt
     6481 gaagatccgg cgcagctgaa cgacccgacc taccgtcgtc tgcgtatcat caccgataac
     6541 ctcaaacagg aagagcacgc cattgtccag gtggaagaaa tgcaggcggt gaatgccgtg
     6601 ctgtatggca aatacaccat ggaaggggag cagtttgata ctgtcgaggt ggatttcggg
     6661 cgctctgaag gaaataacat tgagcaggct gacggtaaaa aatggtctga gcaggaccgt
     6721 gatacgtttg atccgacgca tgatattgac ctctactgcg atcaggccag cggccttgtg
     6781 aatatcgcca ttatggacgg tacggtctgg cgtctgctga atggctttaa gctgttccgc
     6841 gaaaaactgg atacccgtcg cggctcaaat tcacaactcg aaacggcagt gaaagatctg
     6901 ggcgcagtgg tgtccttcaa ggggtattac ggcgatctgg ccattgtggt ggcgaaaacg
     6961 tcttatgtgg cagaggacgg taccgaaaaa cgttatctgc cggagggcac gctggtcctg
     7021 ggaaatacgt cagcggaggg catccgttgc tatggtgcca ttcaggatgc gcaggcgttg
     7081 tccgaaggtg tggtggcctc ttcccgttat ccgaaacact ggatgaccgt gggcgatccg
     7141 gcccgtgaat tcaccatgac gcagtccgct ccgctgatgg tgctgccgga tccggatgag
     7201 tttgtggtgg tacaggtgaa ataatccggg agcgggggcg aaatgccccc gtgtcttttt
     7261 tcacaggggg ctgagatggc aacaaaagaa gaaaatctga atcgtcttcg tcaactggct
     7321 ggtctgctgg ggcgcgaggc ggatatgtcg gggagtgctg cggatattgc tcagcgtgtg
     7381 tctgagtggg aagaggagct tgctgcttcc cgggagggca ttatgcactc tgatgagagc
     7441 gggtctgatc aaaatgacac agacgatggt gagccgttga acaacacgga tgctccggat
     7501 gatgttaaag ccgtccgtgt gcgcaagtgc ctgcatgtga tggggtattg cccggagaca
     7561 ggccgtcctg ttgagcttac gttccggggg atgcgtgttc tggtgccatc atcactggca
     7621 atggccatga tacagcacgg aacggctgag tatgcgtgat gttcagaatg cctttgatgc
     7681 cgcccttgtg ggggtggaca gcacgattgt tgaagtgatg gggatccgtg tgcagtttac
     7741 ctccggagca caacgtggcg gtgaagttca gggggttttt gacgatccgg agtcgctggg
     7801 gtttgccggt ggcggggtcc gtattgaagg aagcagcccg tcattatttg tgcggacgga
     7861 tacggtgcgt gccgtgcggc gtggtgacac gctgaccatt aacggcgaga tgttctgggt
     7921 ggatcgtgtt tctccggatg acgggggcag ctgttatctc tggctcaacc gtgggcaacc
     7981 acccgctgtt aaccggcgac gataaacgca gggtgaaatt atggcgataa aagggcttga
     8041 tcaggcgatt gataatctga gccgggttcg taaaaacgcc attccggcgg cttcagcaat
     8101 gacgattaac cgcgtggcca caacgacgat taatcagtct tcatcacagg ttgcccggga
     8161 gacaaaggta cgccggaaac tggttaagga acggtccaga ctgaaacggg ccacggtcag
     8221 aaatccgaat gccagaatta tcgttaaccg cggtgatctc cctgttatta agctggggat
     8281 cagaatgctg gggcgtcgtc cggacagcat actcaaagcc ggtcagcatc gttatcagcg
     8341 ggcatttatc cagcgattaa ataatgggcg ctggcatgtt atgcaacgtc ttccccaggc
     8401 cagatatgag aagggcaatg acgacaaggg caggaaaaag cgtaatcgcc ttcccattca
     8461 ggtggtgaaa atcccgatgg cggccccact gaaacaggcg tttgatgaaa acgttgaccg
     8521 tatccgtcgt gaacgcctgc ccaaagaact ggcatatgcg ctgaaacaac aactgaggat
     8581 tgcgataaaa cgatgaaaca tactgatatc cgtgcagccg tactggatgc actggagaag
     8641 catgacaccg gggcgacgct gtttgatggt cgccccgctg tttttgagga ggcggatttt
     8701 ccggcggtcg cggtttatct gacggatgca gagtataccg gtgaagagct ggatgctgat
     8761 acctggcggg ccacactgca tattgaggtg tttttaccgg cacaggtacc ggattcagag
     8821 cttgatcagt ggatggaaag ccggatttac ccggcgatgg cggcgatccc tgcactggca
     8881 ggcatgatta ccacgatggt tacgcagggc tatgagtatc gtcgtgatga cgatatggca
     8941 ttgtggagct ctgcagatct gacttattcc attacatacg agatgtgagg atgatatggc
     9001 aacaccaaat cccctggcgc cggtaaaagg ttccggcacc acgctctggg tttacaccgg
     9061 taagggcgat gcttatgcaa acccgctgtc agatgatgag tggacgcgtc tggcaaaaat
     9121 aaaggatctg acccccggcg agatgacggc ggaatcctac gacgataact atctggatga
     9181 tgaggatgcc gactgggtat ccaccgggca ggggcagaaa tccgccggtg acaccagttt
     9241 tacgctggcc tggaagcccg gcgagaaagg gcagcgtgat ttgattgcct ggtttgacag
     9301 cagtgagacc cgggcctaca aaatccgctt cccgaacggt acggtggatg tgttccgtgg
     9361 ctgggtgagc gccattggta aagcggtgac cgccaaagaa gtgattaccc gtacggtaaa
     9421 aatcaccaat atcggtcgtc cttcgctggc ggaggatcgg ggagaaatca caccggtcac
     9481 cggtattacc gtgacgccac taacggggaa tgtggcaaaa ggtcagagca ccaccctgac
     9541 agtggccgtt cagccggaag gcgcaacgga taaaacattc cgcgcaacgt cggcaaatca
     9601 gaatttcgcc accattaccg tgaaagggaa cacgatcacc gtgaaaggtg ttgcggcagg
     9661 taaagcgcag atccctgttg tcaccggcaa cggtgagttt gcggcggtgg cggagatcac
     9721 cgtcacggat ggcgctgcgg gctgagggga ggagatcaag catgttcctg aaaacagaac
     9781 aatttgaata taacggtgtg tccgtcacgc tgtcggagct gtccgcgctg cagcggtttg
     9841 attatatgaa gtttgtttca gacgcagaac aacaggggac aacgaagcat gatg
//
LOCUS       DAZKHK010000157         9881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKHK010000157 DAZKHK010000000
VERSION     DAZKHK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.158"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..1597
                     /locus_tag="ACHOS5_004373"
     CDS             17..1597
                     /locus_tag="ACHOS5_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP9779095.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESNPVTLNVHEDTAIADIPTLDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
     gene            1597..2772
                     /locus_tag="ACHOS5_004374"
     CDS             1597..2772
                     /locus_tag="ACHOS5_004374"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP9779096.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACGREKLYMGLTRLVSVIKNINQGE"
     gene            2775..3371
                     /locus_tag="ACHOS5_004375"
     CDS             2775..3371
                     /locus_tag="ACHOS5_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP9779097.1"
                     /translation="MNNTDLIYLIKHFMHNELKAVEEVIDSPLSEFANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3443..4342
                     /locus_tag="ACHOS5_004376"
     CDS             3443..4342
                     /locus_tag="ACHOS5_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9779098.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDGYQADTLSLLPGVRIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLVGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIVLLPFSSGLENFISH
                     KYLSMMRNGALIINVARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIHVA"
     gene            complement(4690..6066)
                     /locus_tag="ACHOS5_004377"
     CDS             complement(4690..6066)
                     /locus_tag="ACHOS5_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9779099.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDL
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMTGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(6066..6494)
                     /gene="tssE"
                     /locus_tag="ACHOS5_004378"
     CDS             complement(6066..6494)
                     /gene="tssE"
                     /locus_tag="ACHOS5_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9779100.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQISERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(6498..7034)
                     /gene="tssJ"
                     /locus_tag="ACHOS5_004379"
     CDS             complement(6498..7034)
                     /gene="tssJ"
                     /locus_tag="ACHOS5_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9779101.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(7015..8094)
                     /gene="tssG"
                     /locus_tag="ACHOS5_004380"
     CDS             complement(7015..8094)
                     /gene="tssG"
                     /locus_tag="ACHOS5_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9779102.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNASLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(8058..9818)
                     /gene="tssF"
                     /locus_tag="ACHOS5_004381"
     CDS             complement(8058..9818)
                     /gene="tssF"
                     /locus_tag="ACHOS5_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9779103.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFA
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQDRRMLGAHFS
                     PMGFEDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         2436 a         2350 c         2510 g         2585 t
ORIGIN      
        1 atatgagata acccacatga aacaaaagaa agcctggagt ttttttcaga gcctggggaa
       61 ggcatttatg tatcccattg ctctgctaag tgtatgtggc atgatgctag gactgggaag
      121 tggtttagcc agtgatgata tggcaaagtt aattccattt ctggctattc caataattaa
      181 aaccatactt gatttcattg ttagtcttgg tttgtttgcc tttgttaatt tacctgtatt
      241 gtttgcgata gcgattccct taggattatt aaaagataaa gaggataaag cctatggtgc
      301 tttttctggc ttaattggtt ttatggcgat gcatctggga acgaactttt atcttaaaca
      361 gcacgactta ttggtcgttg ctgaccaaat gtcgacacat gggcaaacca tcattctggg
      421 gatccagtcc tacaatacca gcgtgttggg gggaattgtt gctgggttat tagtcgccag
      481 catgtataaa aagatcgtta atttacgcat tcctgaatcg ttaggttttt atagcggccc
      541 acgtctggtg cctatcatta cactgattgt gatgagtgga tttggtctga tcattccttt
      601 tatctggccg ccgtttttca atcttttcat gctcattgga cactggattt caacttccgg
      661 tcctgttggt tatttcttct atgcagttgc cgaacgtgtg acgattcctt ttggcttaaa
      721 ccatctggtg acgtcagttt tccgctttac gccaatcggc ggttcggctg tgattggcgg
      781 cgaagaatat tacggcaccc tgaacatgtt tatggcatac gttaaagaga atgcggtcat
      841 tccgctggat ttggcgggga aaatggagca gggcaaactg atgattcagt atggtctggc
      901 tggtgccgcg ctggcgatgt atcgcactgc tcatgctcaa aacagaaagg ctatcaaagc
      961 attgcttatt tccggggtgc ttacggtgat tattggcggc gtcagcgaac cgattgagtt
     1021 tctgttctta tttgtcagtc cactgctgtt tgtcttccat gcctttatga atggattcgc
     1081 taacatggtt ctgccatata tgggcgtgaa gatgggattt actggcgatc tgattcaatt
     1141 tattagcttt ggcgtattgc gtggcacaag aacaggttgg ccgatcgcgg tgtgtgtcga
     1201 agtggcctat ttcttcattt attactttgt gttccgttgg accattctta aatttaacct
     1261 gatgaccgta ggccgtgaag agtccaatcc tgtcacgctg aacgttcacg aagatacggc
     1321 tatagcggat atcccaactc ttgataaatc agagctgcaa gcggcggagc agatggttaa
     1381 ggcacttggt ggtaaagaga atattaagtc actggataat tgcgtaactc gtttacgttt
     1441 aacaatcgca gatatgggat tgattgacga agttgcaata aaaagagctg gcgggattgc
     1501 ggttgttaaa cttgatcaaa ataccctaca agtcattatc ggcactaaag tcatcgccct
     1561 gcgtcgggat atggataact atatggggat atactgatgg atgtttttaa tactcctgtt
     1621 tcccgcaaag gaacatattg tacccaatgg gacttttgcg aggacagatt tggtgtaaaa
     1681 gatgtactac cattctctat ttcagatatg gatcttccga taccagacgc tatcaccaga
     1741 gcattaaaaa aacgcctgga acacccgatt ttagggtaca gtcgttggca acatggtgaa
     1801 tatctgaatg cgatagtaaa ctggtattat caacaatatc aaacagatat taaacctgaa
     1861 tggataacct atagccccag tgtgatgtac tctattgcaa aagcaataga gttattgaca
     1921 tcgcatggtg ataatattct cgtttttaca cctgtctata atgcattttt tgatgtaatt
     1981 aaacatagcg agcgaaatat tctgacagcc tctttgatta taaaaaatga ggggggctac
     2041 gctatcaact ggcaagattt tgatcttaag attaaaagcg caaagatggt gttattatgt
     2101 aatcctcata acccgacagg aacagtctgg tcggaagaag aactgcataa aatcgctgca
     2161 agctgtacaa ggcataatgt ctggttatgt tcagatgaaa tccatagtga ttttgttttt
     2221 aatcgtagct ttacatccgc gcttaaaata aaaaaagaaa aagtagtagt atttaactcg
     2281 atatcgaaaa catttaatgt tcctgcacta acaggatcgt atatgatttc aactgacgat
     2341 aattttaatc ataagttcag aacgatatcg aggtatcgcg attttgtaaa ctccccatca
     2401 gtactgaata taattgcaac tattattgcc tataacgaat gtgagggatg gttaaagtca
     2461 ttaaaggcac atattgcctc taacattcaa ttcacccagc agtatctgaa tgaaaatatt
     2521 ccagaattga tcgtaaggcc agctgatggt tgctattttt cctggataga ttgttctgct
     2581 attggttatc cttttgatga attctacagt cgattaattc atgaaggcaa agtgggcatt
     2641 atggcggggc atgtctatgg aacggagggg gagggctacc ttcgtctgaa cctggcctgc
     2701 ggtcgagaaa aattatatat ggggcttacg cgtcttgtga gtgtaattaa aaatattaat
     2761 cagggagaat aaaaatgaat aacacggatc ttatctatct cattaaacat tttatgcata
     2821 acgaacttaa agcagtagaa gaggttattg attccccact ttctgaattc gctaatttaa
     2881 ttaaagtatt acaatcttgt cagggaaaag ttgtttttat tggtgttggt aagtccggta
     2941 ttattgccag aaaactcgca gcaacttttg ccagtaccgg aactccctcg ttttttgttc
     3001 acggtacgga agcggtacac ggcgaccttg gaatggtggc gaaagacgat gttgttattc
     3061 ttatttcaaa cagtggtgag acggcggaaa tcctggcgac actgcctagt ttgaaaaaaa
     3121 tgggtaatta tttgatctct tttacccgca gtcatcactc atcgcttgcg ataagttgtg
     3181 atttatctgt tgaaatcccc gtcaaaagtg aggcggataa tttaggttta gcaccgtcat
     3241 gctcgtcgac tgttgttctg gttgttggtg atgcggtggc gcttgcactc tctgaattga
     3301 aaaaatttac tcgtgctgat ttcggcttat atcatccagg aggtgcactc ggcattaaag
     3361 caaattcata atttcaaaat ataaatacag taaccggtca cgccatcttt tggggtggcg
     3421 ttctttgaag tgagtgtccg ctatgaaaaa tgttctggtc accgtcccca gcttttcagc
     3481 acgctgtgtt tccgccagca agttgctaag agagaataat tttaatctta ttattaagaa
     3541 taatgtggag catctgctta aatcagaatc cactgcatta cgagaatcta tttgcgcggt
     3601 catcgccgga aaagatggtt atcaggcgga cacgctatca ttgctgcctg gtgtgagaat
     3661 aatatcgaga ttcgggacgg ggatagacaa tattgatctt cgtgcggcac agcaatccgg
     3721 tattgttgtc aataatgcgg ttgggattaa ttcaaatgcg gttgcggaat ttatcattgg
     3781 acttatcttc gcgagcatga gaaatatccc tggcagctat catgcgatgc aaaatggcta
     3841 ctggggtgag tcgcatggct gtgaattaca agggaaacgg attggcctgg ttggctacgg
     3901 taatataggt aaaactctgg cgaaaaggtt atccggtttt gatgttgagc ttttagcttt
     3961 tgacaaacaa cccgactatc aggttgctga caaagctgga gttcagtttg tatcaattga
     4021 agatatcttt atgcagtcgc atgtcatcat tgttcttttg cctttttctt ctgggctgga
     4081 gaacttcatt agtcataaat atttgtcgat gatgcgcaat ggtgctctga tcatcaatgt
     4141 cgcaagggga aagttgctgg atgagggcgc tttacttcag gtgattgaag aacgaaatgt
     4201 gtttgcggcg ctggatgtgt ttagctctga accgttggcg caatttagcc cactgctaca
     4261 tgccaaaaat atcattacaa cgccgcatat cgcggccgcc accgttgagt cataccagca
     4321 aaccgggata cacgttgcct agtcgataat tgattacttc gcaggaaggg agataaaaaa
     4381 cgtgttgtga gttcagcgaa caaagatttt aaatacaaaa aaaccgcaat ttgtagaaca
     4441 cattgcggtt tgtcagcatt ctaataaatt ggttgcgggg gccacagtca agaggttaat
     4501 ccaatctttt gaaacttaag gtattcactg ttcccgaacc caagctatct accgaaatat
     4561 acactcatta tctgatgtga atcaacgagt agatcgaatg gcctgttcct ctcttttttt
     4621 gtacacagag actcctttaa aaaatggcaa ggtcacatat cccttattgg tacataaatc
     4681 ccccgatgtt tactgacttt gttccacctt cgtatccagt aaaatgacat agcgattcaa
     4741 caactgattt aaccgggtat caatttgcgt caacagcgtt ggcgaaacct ctccggtctg
     4801 tttttgctct tccagttgcc gaagcagttc ttccagcggc ggctccttta acgactgccg
     4861 gatgccgtac accgccgttt ttagttccga accggtcaga tatctcccct tacgttcatc
     4921 cagtgcatcc agctgcgccg acaggcgctg tagctggctc tgcgcctgag catagccatt
     4981 cagtgcctca gcagacaggg cgctcagctc ccgctgtttg cgccactgcg cgttcagcgc
     5041 cctgacctga ggattatccg gccacagggc atcaagctgg cgtagctggc gatatccctg
     5101 ctccagtggc cagcgggcag gcaatttgtt taactgctcc agttgcatct gacttgccgc
     5161 aattgttctg tcctgagcca gcagcgcctt atcctttaca tccagctttg ccagttcgcc
     5221 gctctccagc gacagcggca acggcatgac agacagttgt attcgctgga ttagcgcgtc
     5281 cggctgatta agctgcgacc agccccataa tcctgttccg ccgagaccgg ccatcacaac
     5341 cattccggtc ataaatatcg gccatgtccg ctgccgggag atttcagggg atggaacggg
     5401 tggactggca tccttatcgt tttgctgaac ggatctggtt gcaaaaaccg gctgtggact
     5461 ttcctctgac tttttcggtt gattcatttc ccggcggggt aattcagggg gagtgatagt
     5521 ggtttccgct ccctgaggcg ccagccgttc cagacgcaat gcggtattgc gaaactgccc
     5581 ggccagtaga tccagtttcg tcatatgcca cagttcacac tgttgcagcg tggtttcgat
     5641 ggcggaaaga tgctgtacac aacggtagat ctgcggcaga tcctgatact gaatatccag
     5701 cgttctcagt gcctgctgca ttttttctgt gacccagctg agcagtgata ttctggaatg
     5761 tacctgtacc ggccagaagt cagcccagcg ctggagcagt atatccagtg aaccgagtcc
     5821 gtctgccatc cctgccagcc cctgccgtct ggttatcgcc agtacgtaac agaccagcgt
     5881 ctgtagttcc acgccatttt gccggaacag cgcgaggcag agtttttcca cctgacgcca
     5941 gtccacatcc ggtcgggccg ggtgagtcag tttactcatt tcagcctgca gttcaccgga
     6001 ttccttataa gtacagggat cgccgcccat gacgaactgg ctgataaaat tcgcgttact
     6061 tgccatcagt acgcccgctc ctgttgtttg agatgacgca gaacaattcg tccttccgga
     6121 gaaaatacgc catcaaagcg tatccagccg gtatcgggaa gctgcgcgtg gatcaggtaa
     6181 ttaagatgcc ccggacgggg ctgggggagt aattccacct gtatctgact caggcgtggt
     6241 tcataacgca gcagtgtttc ttcaatctga cgcatcaggc catgaatacc cgcagccatc
     6301 ccctgatgga tcagactgag atccggcaga ccgtaatccg gcaaatgcgc cagcgtgcct
     6361 gcccggcagt tgataatgcg ttgcatattg tccatcacgg aaaggataag ctgatcccgc
     6421 tcgctgatct gttccagcgg taactcgccg ctgaaactga acgtcagcat ttcatataag
     6481 gaaggtgtcg acatcggtta tttatccttg agaggcagca gtcgcaggtc gcttctcatc
     6541 agttcaatcg agcgcggctt gtcggcctcc agttcgtccc ttttgatcac cagacgccag
     6601 ctgtcgcttt tttcatcagg gtgataaaac tgcccaataa tggcgacaaa ctgcgtctct
     6661 ttatccagcg gcacattcag ggatgccgtg ccttccggtt ttacccagac ggtgtgtttt
     6721 gccagtacgt ccttctccag cgtcttctct tcctgcataa acagggtgtc gtaatccgcc
     6781 ttgtcaaaat cttcgcgggt tttcagttgc cagacatgca ccatcgtgga aagcggcgta
     6841 tcttccgcat ccgtattcag ggcgctgcgg gagaagaaat cgagatgcag tattttgatt
     6901 tgtttgtaga aaacggctga ggccacgctt ttcgattcat ccaccacttt ctgaatcagc
     6961 ccgcagccgc tcagaacaac cgtcagcatc gccatacaga gcaacgccgg acgtttaaaa
     7021 cgaataggaa acatgagtca tactctccct gttacgaatg gaaaaagcgg gtattaatcc
     7081 ctgataacgc cccagactga ccgtcactgt tccggtcgcg ggcgcggcaa aggaggcgcg
     7141 aaggatgccg gtacggctaa tctgtacacg ctgttttccc agccgggcag caggcaacag
     7201 gctgacgggc agcgtcagtt gcagacgggc ctgacagcgc cagcccagat aaacgcgcag
     7261 cagcaccagc aaatcgctgt gcagttgccc gccgggcagc cagccacgtg cttctgcggc
     7321 atcttcggta tacagtttca gcagcaactg gctgttgacg tcggtaccgg tacgccccag
     7381 cagcgttcgc gttttcaggc tgatgcgtga attttttgac agacccgcag gggccgggag
     7441 aattacaggc tgcggatcgt gaggcaccac cgtcgctttg gtcagcgggg caagcagccc
     7501 taccagtgcc gtgacgcctt ccgccgtgcg cgtcggcagg cgcatcacgc tgagcagggc
     7561 cagaaaacgg gataccggcg tggcgatatg gttctcgctg ccgggaatac ccagcccgat
     7621 gagtcccagc agacaacggg aaatgtcatc cacggcacct gcttcaaacg acgccgggta
     7681 gttatatttg cgccagatgc ggtagtactg agtaataaag cgatggttaa aaatatcgag
     7741 aaacgccatc actgcgtcat gtccgtcatg acgctgggta atgtagtcca gataggcggt
     7801 tggcagcggc gaatccacgc cgtacaggcc gagaaaagtg gtgcgcaccg tggggggcgc
     7861 gtcgggattg cccacgtcac gttcaacgtt ttttaactcg ctgaccggaa agcccatacc
     7921 tggatgagga cgaaagcgca ccgcatcggt ggcggggctg tcggtactgc ccagcgacgc
     7981 gttctgctgg ctctgttcca gcaactggca aaaacggtaa aaattgatcc gcgcaatatc
     8041 cggggccagc cggtcggtca gcccggtacg cgctggctgt gatgctctgt ccattgcagg
     8101 cactttcctg tgggttgcag aattaaggtc agttgagtga ataagtggat atcggcatac
     8161 agtgcgaaaa agcggtgcag cagttcacca aacagcgtga tatcaccttc tccggcaaag
     8221 ccgttactgt ccagcgtcac ctgaatatcc acgccacgca gcaaaaagcc tctttcaaaa
     8281 cgctgtatca ggctgtgctg aacgtcgaca atcgcctcca gacgacggcg gttcatctcg
     8341 ctttcggtcc agtcgtacaa tgccagcgtt ccgcgcagaa tttcggcgtt atccatcatc
     8401 gataaaaagt tagaaccaag gtgactgagc acccgccagt ggaagcggtc acgcgccggg
     8461 ggatagcagg gctgcgtcgg agcgcacagg ttgcgcaccc gcaggacgtt ctgtgaggca
     8521 tgaactggcg tatcgagcag cgtgctctgt aaagccttac ggggaagctg cccgttagta
     8581 ccggtcaggc ttaaagagag cgtttcatcc tccagcatcc ggtcagtatc aaatgcatcg
     8641 ccgcccagaa tcagccaggt gtcatgcagg ccggaagggc cgcgctttac ccgcgtgtgg
     8701 taatagcgtt ccggcgcatc gtggcgcagc atcccgcccc ggtgacgaaa gctggagaag
     8761 gggacatacg cctgactgcc ggtatggcgc gaagaaataa tgttgtcgac ggaataaatc
     8821 tcggtatgcc cgtcctgaat gcgcatcggg cgcagcagaa attcgttttc cagtggcgaa
     8881 aggtgtaacg gatcggcttc gagcgggaac aggttgatga ccggcacgca gtgcagacgg
     8941 atattatcgc tgtcaaacgg caggtcgtga ggccagttct cgttcagcac cacgtcaatc
     9001 tcaaagccgg ttatcccctc cggccatgca acctgctcca gcccgttcag cgccacgaac
     9061 ataaacttct cgcggaaggt gaaatactcc agcagcaact ggtaaccgct gaaggctgac
     9121 tcaccttttg gccagagcct gtcatcatcc tcaaagccca tcggggaaaa gtgtgcgcca
     9181 agcatccggc gatcctgccc cggcagacgc agccagaact gctgcgtgcc aagcgttaaa
     9241 gcccggtgca acgcacaggc taccgggctg tcggcattga gatagagcgg caggcgggat
     9301 aaatcgatct gcgaccagtc accaaccagc gggccgcacg caaagcgcag acggataacc
     9361 gagcgtccgt ccggttcata ttgcaatgac acgtccggca acgccagcgg atgcagcgtg
     9421 atatcacgcg tggcactgta gacgcagcgg gttctgcgct cgccaacagg gcgcgacaat
     9481 acctgaaaat cgctcagcgt ttcgctctgc ttcatctgcc ggtgatctgt cgacagttca
     9541 accacggaaa gtgacgggat ggttcgcagg tagtggggcc acagcaggct gaccagccct
     9601 tcagtcagtt ccggcagatc gtcgtccagc ttttcgcgca ggcggcccat taaaaaagcg
     9661 aagccctcga acaggcgctc cacataggga tcgcgtgcgc cgggtttatc aagattaagc
     9721 atcgcagcac ggtcggggta agcctgcgca aattctttac ccgcttcacg caggtagcgc
     9781 atttcggctt catagtagcg ttgggtcagg tcgtccatcg gaggatttat cttcctttta
     9841 ttgaaattaa taccaacgag tagtgagatt agaatttttt a
//
LOCUS       DAZKHK010000158         9869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKHK010000158 DAZKHK010000000
VERSION     DAZKHK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.159"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(18..221)
                     /locus_tag="ACHOS5_004382"
                     /pseudo
     CDS             complement(18..221)
                     /locus_tag="ACHOS5_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            299..1393
                     /gene="ddlA"
                     /locus_tag="ACHOS5_004383"
     CDS             299..1393
                     /gene="ddlA"
                     /locus_tag="ACHOS5_004383"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9779104.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1417..1629)
                     /locus_tag="ACHOS5_004384"
     CDS             complement(1417..1629)
                     /locus_tag="ACHOS5_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP9779105.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1889..2197
                     /locus_tag="ACHOS5_004385"
     CDS             1889..2197
                     /locus_tag="ACHOS5_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP9779106.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYEHLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2256..3350)
                     /gene="yaiW"
                     /locus_tag="ACHOS5_004386"
     CDS             complement(2256..3350)
                     /gene="yaiW"
                     /locus_tag="ACHOS5_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP9779107.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3363..4583)
                     /gene="sbmA"
                     /locus_tag="ACHOS5_004387"
     CDS             complement(3363..4583)
                     /gene="sbmA"
                     /locus_tag="ACHOS5_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP9779108.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4935..6092
                     /gene="ampH"
                     /locus_tag="ACHOS5_004388"
     CDS             4935..6092
                     /gene="ampH"
                     /locus_tag="ACHOS5_004388"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP9779109.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYIAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6093..6716)
                     /gene="iprA"
                     /locus_tag="ACHOS5_004389"
     CDS             complement(6093..6716)
                     /gene="iprA"
                     /locus_tag="ACHOS5_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP9779110.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6802..9750)
                     /gene="ehaB"
                     /locus_tag="ACHOS5_004390"
     CDS             complement(6802..9750)
                     /gene="ehaB"
                     /locus_tag="ACHOS5_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP9779111.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2547 a         2450 c         2337 g         2535 t
ORIGIN      
        1 atataggcct ttaattatca acctttatta taaataaaag ttatgaaata tattctcttc
       61 gttatcttat aattaagaga aaaaaactca ccacatggtc atttaagcca ggaataaatt
      121 agcttcccgt tcatcatttt tgtgatcttg cacacatctt tgagataacg cactttcttc
      181 accagtacgt tgctcgcttt gtcagcagaa gattgcaaca ttctgtcaga aggccgcgaa
      241 tcccccgttt tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat
      301 ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct
      361 gcaatcggca aaaaacattg tcgatgccat tgataaaagt cgcttcgatg ttgtgctgct
      421 gggcattgat aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc
      481 agacgatcct gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg
      541 taaacatgag catcagctta tcgacgcaca aaacggtcag ccgttgccga cggtggatgt
      601 cattttcccg attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg
      661 ggtcgccaat ttaccatttg taggttctga tgttctggct tcagcggcct gtatggataa
      721 agatgtcacc aaacgtctgc tgcgcgatgc cggactgaac attgcgccat ttattaccct
      781 gacgcgcgct aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc
      841 gctgtttgta aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga
      901 agaacagtac acaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga
      961 gcaagggatc aaaggtcgtg aaatcgaatg cgcagttctt ggcaacgata acccgcaagc
     1021 aagcacctgt ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat
     1081 tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt gcgccagaaa tcaacgataa
     1141 gatccgggcg attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt
     1201 agacgtgttt ttaaccccag agaacgaagt ggtgatcaac gagatcaaca ccctgcctgg
     1261 cttcaccaac atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga
     1321 cctgatcacg cgtttgattg agctggcgct ggagcgtcac gctgcggata acgcactgaa
     1381 aaccacaatg taatattcaa acgcccggta atattcttat tgggcgtttt cagtttcttc
     1441 atcgcgacga cgaataatca atcccgccag ccagaaacta atcacccacg tcaccagacc
     1501 gacggcatag gtctgccaac cttttgcttc aaatcccagt aaccccacca cgccattaag
     1561 aatgaatata aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg
     1621 cagattcatg gcagctccgt taacgcaaaa caacatcctt gcatattctg taacaactgt
     1681 ctgtggtgta gacaacactt tcgcaattgt taataaattt aacttaaggt tgctattttg
     1741 tgactcacaa cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct
     1801 gatattgaca atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa
     1861 gagaagcaga tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt
     1921 tagcggaaaa tatcgcaatg cctcttcaac gcctggcaat attgcctatg cgttgtttgt
     1981 gctgttttgc ttttgggctg gggcgcaatt gctgaacctg ctagtgcatg cgcccggcgt
     2041 ctatgagcat ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg
     2101 tgtcggcacc atttttgggc tgatcccgtt tttagtaggc tgcctcattt ttgcagtagt
     2161 ggcgctatgg ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatgcggcc
     2221 taaaacgcct tatctggcgt gacattcctg tttgatcact gtttcataca acgcgcccgc
     2281 cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcagat tgcgggtgat tttcgggctt
     2341 tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt taccggtttt cgcttcggca
     2401 agttgataaa ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc ccctttctct
     2461 aactgacggc gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc
     2521 agttcggttt tcccgggctc tttactgtcg taacgaatta aatcaccgtc cagtgccagc
     2581 ttcacgccgc tggccttact gaccgcgttc tgaaacgcgg cattacggct ggcgtaccag
     2641 ccagcgttaa aatcagcaaa acggtatatc ggcgcgctat agctggcggg ataattcagt
     2701 aaatggtaag taccaaacca caacccgccg cggcgactga agacttcctg acggactgta
     2761 ccgtccattt tccacggata ccctttggta tgctgttcgg caaaagcaat actgacctgc
     2821 atcggcccac cggtgcgcac cggattgagc gaaccaaaca gcgtctgccc cattggcacc
     2881 atgctgatca gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac
     2941 cgttcgctat agcttttgcc gtttggcgat ttgattttta gcgcggtatg caccagaaaa
     3001 gcaggaatgt gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag
     3061 cccggaacgg ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag
     3121 cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc gagatctttc
     3181 gcccaggcat cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgccaca
     3241 tctatcgctt tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa
     3301 accagaaccg ccaacaatga cagcgaagat agaggattga cgcgcgacat tacttctcct
     3361 ttttagctca aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag
     3421 ctgcgcagac gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg
     3481 tactggaaag caccgcgaac ctgaccaaaa acgttagtaa tctgcgtcat caggccgagt
     3541 gtaatcgtac cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc
     3601 tgcaaataga gaatgcgggc gatattgaaa tacatatagt gaaaatagag gcggaaatag
     3661 tttttccgta cggcgctaaa aagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg
     3721 tcttcaccat aaaccagctc tttacggtag gcagcctcta cacgctggtt tttaaactcc
     3781 agccccggca gtttgatccc taccactgcc agtaatccgg tccccatcag cgaccagacg
     3841 atagcggcaa tcaccagacc atacggaatg tgcccgacaa tcggcagctc cggcacatgt
     3901 gcggagagcg ttaccagcac cggcaggaag gcgatcaacg tcatgatagc gttgataaag
     3961 ctgacgccca tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgc
     4021 gcggcccctt cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc
     4081 gtacgccagc ggaacacgta gtgactgaca aagaagttgt tcagcacact gatcaccacc
     4141 gcgatcagcg caatccccag aaagacgccc acttcgcggt aaaattgttc gatggtgact
     4201 ttatgcggcg aacttagcgc cgtttgaatc agatcataga acggcgcata ccaggcgttg
     4261 acggcaaccc cgacttccac caaaaaccag gtgacaaaga taatcagcgc agtaccgagt
     4321 attgaccagt attgccaacg gtgcgggcta tagataaacc agaacaatgc aaaaagtcct
     4381 acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg
     4441 ctaatcggga tctgcccgga agcgccggtg atacgcgcca cccaatcccc cccaccggct
     4501 tgccagaaga taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgttcccggc
     4561 tttgggaaaa aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg
     4621 ctgtgtatta accgcatgac cgctacaaag cgtcagccaa atgaaaccgc ccattgtgac
     4681 ccatagcaag gcgcttagtt cgccagcctg accataagaa ttgtttcgac gggtttcaca
     4741 aaccaaagat tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg
     4801 tgagcaggaa aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa
     4861 ttcgcccttt gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc
     4921 tatggacacc accgttgaaa cgtagtctgc ttttttctgc cgtgctgtat gcggcgtcat
     4981 tgacctccgt ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg
     5041 atcgttatgc cgatcatatt ttttacggca gcggtgccac ggggatggcg ctggttgtta
     5101 tcgacggtaa ccagcgcgtc tttcgcagtt atggcgaaac gcgacctggt aataacgttc
     5161 gcccgcagct ggattccgtc atacgtattg cttccctcac caagttaatg accagtgaga
     5221 tgctggtgaa attgctcgac caggggaccg tgaagttaaa cgatccgcta agtaaatatg
     5281 ctccgccagg cgcacgcgta ccgacctaca atgggacgcc cattacgctg gtgaatctgg
     5341 caacccatac cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtat
     5401 ttgtctggcc aacgcgcgag caacgctgga aatacctttc cactgccaaa ctaaaagccg
     5461 cgccgggcag ccaggccgcg tactccaacc tcgcattcga tctgctggca gatgcactgg
     5521 cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccgttgg
     5581 ggatgaaaga caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg
     5641 agcgcggtgc cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt
     5701 ccacgccagg cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc
     5761 gcagtaacca ggctgatcgc atgcagacgt tgatttatca gcgcgcgcag tttacaaaag
     5821 tgattggcat ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg
     5881 ccccgaaaga gggtcgtccg gggattattc agaagacagg tggtggtggc ggtttcatta
     5941 cctatatagc gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc
     6001 cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga cctggtgacc gagctaagcg
     6061 ggaataaacc gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata
     6121 gcgaccagtt tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact
     6181 tccgcaacga cagaacgcga tatacgtgta cgttgatgga tataattcat cacgccaata
     6241 cgtgatcgca attcttcatt ccagtcaatc attgataata acgtcgcgcg gatttgttcg
     6301 taggaattat gcccaatgag ctgaacgtcg cgtaattcca gaattctatt ttgccaggct
     6361 aaccagtaaa aagcgtctcg ccagagttga ttttgttcaa taagagtaat ggtttgtttg
     6421 gctggcaaat gatatcccgt acagtttcct tctgatatta atttgtatgg tatatcgttt
     6481 ttcattaaac catcagccag ccccataata taaggagcct gtgtaatacc gataagtacg
     6541 ttttcttctc tacgcaaaga gataacgccc tctaaaataa cgaaagtatc ttcgctattg
     6601 acatcactgg aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg
     6661 gacaaacatt tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc
     6721 gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacct
     6781 atttcttcgg agtgaagaat attaccaggt atatttaaca cccacattcg cggaccagtc
     6841 ttgatctacg tcaccaccac cgaggtagtt agcatcggta taggcgctga agttcttggt
     6901 gaagctgaac tgagtaccca gaccaacacg taccgcagac ccttcagtac cgttatcgat
     6961 ggaatcaccg ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag ccagtttgaa
     7021 gtacggagtc agagcctggt cttcgctgta ggtgaaggta taacctgcat ctacacccag
     7081 ttcataacgc atgctgtcgt aagactgacc atcaactttc atgtcgttgc tcagctggta
     7141 atcatcacca gactggaaca gaccagaaac gctgccgtaa ggagtcacgt aacctgcatc
     7201 acccagtttg aagtcgtaac cggctttcaa accaaagccc caagcgtcgg agttggtgct
     7261 gccgtcaacg taagtaccgt tgctcatggt tgcagacagg tcgttgttga agtgagagta
     7321 gctcaagcta ccgtcaacaa agacgttgtt cgcgaagtga gcagaagagt agatgtaggc
     7381 agtctggctg tcttgatcca cctgaccaga acggtcattc atatcacctt tagcgaagcc
     7441 tgcagccgca ccgacgatcc acttagcgtt gttaccgtca attttggtat caacaccgac
     7501 catgatgccg ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt tgaagttacc
     7561 accgaagtag cttacccatg cgccgccgtt atcagccaga ccatgacgag agttggtcag
     7621 acgagtacca acggtgtctt gttccaggtt ccagatattg gtgttcgcag acgggatgct
     7681 cagcgccatg ttagcgtagt cggtcagctc catctgttgc agaacaacgg tgttaccacg
     7741 ctgctcagcc tgataggtgt atgcccccag gtcagctttg ttagcagcag agaaggtcgc
     7801 gttgctgttt ttgtcgttga cgtagatcag ctcgttaccg ttgtagtcag cgatagaacc
     7861 agcaccagta gcgttgtcga tacgaacttt gtagttaccg gttgcagctg caacatggtc
     7921 accgttgtta tcaacttcgt tctgaccagc ttcgatagaa gcagtgttgt cgttatcgcc
     7981 gttaccgtta atggtcaggt gaccatcaga gttcattgcg attaagccat aaccgtagtt
     8041 tgacttggta gtatcgttgg tacggtcgtt ggtcagatcg gcatcgagta cgaagcggtt
     8101 gctttcgatg ttgaacacac ctgcgtgaat gttaccactg gtatcagtag tgctggtcag
     8161 gttcagagta tcaacctgca gagcattcgc cgcagtgtta cctacatcca ggataccgtc
     8221 gttagtcact ttgatgttgt tagcatacag agcagcatca cctacagccc agccagcaga
     8281 aacttcatca gaaatggtta cggtactgtt gtcgacagtc agtgaatcag ttgcaacatg
     8341 accatcttcg ttgatgttca gcgcagaaag accagtcaga gagatagagt cagaaatcag
     8401 cgttgaatca gcaacattaa cagttgaacc actgttgatg ctcagagtat caatcagaga
     8461 agttttggtg gtatcccact gtgaaccggt gttcagggtc acattgaaca gaccgctctg
     8521 gtaaacttcg ttaccgatga tgtgatcaaa ttcgctgtat tcagagtcgc tgttttctac
     8581 accaacagtt gatgaaggcc acaggctgtt agctgcgatg tcgatcagag ttgcagtggc
     8641 ttcagtattg attccatatg tataggagtc atttacacca tcgtcatcag tatcaacacc
     8701 aatctgatgc gcagacattg ccgcaccgac ccacttgctg ccattgttca gggtcagatc
     8761 caggcgatca gtaccatccc aaccgttagt atctacatca ccgtcagcat cacgatagct
     8821 atcacgacct tgtgggaaga agttctcatc aaaattactt gagaagaata catcgcccat
     8881 cagtgtggaa ttgtcgaatg ttgcagtggt ttgcatggcg ttatcagcat atggatgtgc
     8941 aataacagct aatgcaacat catcagcatt ccacccatca ccattgtaat cactagcatt
     9001 accagtatta ccaaaccaac cagtagtacc ttcatcagtc catgaaccag aggtcacggt
     9061 agaatcttta acagtgatgg tgttattaaa tacttcgctg ctgtttacac cagtgctgac
     9121 agtgttgtcg tcgatatctt cccactcata accctgagtc agcgtaatac ctgctacatg
     9181 agagttatta gagatggaca gatcaacttc ctgatccaga gtgatggcag taccaatgtt
     9241 aaatacattt acaacatgag tgtcagcggc attattgtag gtgccgttat atgtgtagtg
     9301 ttcgtagttg tcatcgatcg ttgaattatc aacggtcagc gccagacgat catggtagta
     9361 gtcatccgca cgatcagcac aatcagttgt catgcactcg gaataaatca taccgtgaat
     9421 agtgctgttt ttgattgtca ggctattggc gttggtatta gtagaaatac catcgtccag
     9481 atagtaggta gaaatcacgc cgttaacggt agcgttatta atgaccggat agatatcgcc
     9541 gttgtaataa ctatcagcgg catagttatt ccagcctaca taaccatcat agtaaacgtc
     9601 atcagcatag gtggcgtcgt tgtagtgatg gaaagtattg taagtttgac cagaaatatc
     9661 gttggtcgct gcattagcct gagaggtgat agccagagtg caagccaatg ctaattgtga
     9721 tactacaagt ttctttttcc aggagtgcat ttgtcatccc tcctcaggga cgtaataagt
     9781 tgatccatca attatcaatg catagaaaac atattatgtt tcgcatttac gattatgcgg
     9841 cgttatagag gaatggttca attattctt
//
LOCUS       DAZKHK010000159         9739 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKHK010000159 DAZKHK010000000
VERSION     DAZKHK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.160"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1034
                     /locus_tag="ACHOS5_004391"
     CDS             <1..1034
                     /locus_tag="ACHOS5_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HFP9779112.1"
                     /translation="LSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKF
                     LTLHKLSEKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFE
                     HSSIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLK
                     KQIKSQREMVCYSALVAINNDMSFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNL
                     IKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGN
                     LTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMK
                     TQLKKLWLV"
     gene            complement(1103..2059)
                     /gene="yjiA"
                     /locus_tag="ACHOS5_004392"
     CDS             complement(1103..2059)
                     /gene="yjiA"
                     /locus_tag="ACHOS5_004392"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9779113.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGYIQFDRLVIECTGMA
                     DPGPIIQTFFSHEVLCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(2070..2273)
                     /locus_tag="ACHOS5_004393"
     CDS             complement(2070..2273)
                     /locus_tag="ACHOS5_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9779114.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2391..4541)
                     /gene="btsT"
                     /locus_tag="ACHOS5_004394"
     CDS             complement(2391..4541)
                     /gene="btsT"
                     /locus_tag="ACHOS5_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP9779115.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFISALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVIPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            4918..6582
                     /gene="tsr"
                     /locus_tag="ACHOS5_004395"
     CDS             4918..6582
                     /gene="tsr"
                     /locus_tag="ACHOS5_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP9779116.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
     gene            complement(6625..7896)
                     /locus_tag="ACHOS5_004396"
     CDS             complement(6625..7896)
                     /locus_tag="ACHOS5_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9779117.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(7893..8366)
                     /locus_tag="ACHOS5_004397"
     CDS             complement(7893..8366)
                     /locus_tag="ACHOS5_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9779118.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            complement(8430..9401)
                     /locus_tag="ACHOS5_004398"
     CDS             complement(8430..9401)
                     /locus_tag="ACHOS5_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFP9779119.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         2582 a         2324 c         2478 g         2355 t
ORIGIN      
        1 ctttaagtaa tgcagatctt ttaaaaaatg cactgtttga atcagcttct acgcataata
       61 aaaaatcaga gcaaatagaa tcagcatggt cgcaaattga agatatggtt ggagtgagac
      121 ggcttgataa atttcttact cttcataagc tttccgagaa aaaagatcgt gacagggtgt
      181 tacaaaaagg ttttgaagct tttattgaaa atctccagca gcaatttgat ggagatgcta
      241 ttgctatgtc actgatgttg gttaattctg caaaaaatta tactaagatt ttagagaatg
      301 attttgaaca ctcttcaata cgacgaaaaa tagcatcact aagtaattta ggggtagatg
      361 agtggattcc tccggttatg gcattcatga atcgaatggc tcgtacagaa gattttaatt
      421 tagatgattt ttctcaattt attaccgcct ttgagaaggt atatatgcat gggtggttaa
      481 aaaaacaaat taaaagccaa agagaaatgg tttgttattc agctctggta gcaataaata
      541 atgatatgtc ttttgattct gtgataaatc agataaacca acatgcagat aattcaggtt
      601 ttatagctgc tctggatgaa gatttatatg agcctcgtcc gaatcaagtg aacttaataa
      661 aagcaatttt gcttcgctta gatatggagc aacaagatga gagcgtgatt aaaacctata
      721 cggggagaat cacaattgag catattcttc ctcaagcatt agttaatgaa tactggatta
      781 atagattcca acctcaagag catgtctact ggctgcataa aataggtaat ttaacactca
      841 taagtggttc caaaaattct gaagcacaac actatgattt tatcaaaaag aaatctattt
      901 atgagaaact taatagtaaa agctcttttg atttaacaaa agatgtttgt aattcttcag
      961 aatggggatt ggctgaactg aaaatgagac atgaaaaaat gaaaacgcaa ttgaagaagc
     1021 tttggttggt ctgacatatc taaagagttc cactcgtaat aatgttgcgg tggaactctc
     1081 aaaaagttga tctttcccga acttacttcc tcaacccagc aaacgccgcc cgaatctctt
     1141 cttccggcag ctgaatcccg ataaacacca tcgtactatg cggtttttca tcgccccacg
     1201 ggcggtccca gtcggcgctg tagaggcgtt ggacgccctg gaacagcagg cgattaggtt
     1261 cgccgtcaat ccatagcatc cctttgtaac gcagcagttt atccgccgat tccagcagca
     1321 ggttttccat cacccgggaa acttcgctga tatctaccgg gtaatccagt tccaccacaa
     1381 tcgacgaaat atcgttttgt ttgtccgcga taaagtgaaa acgtggcttg gtgctgacga
     1441 cgttttcttc cagcataaaa ccgttggtgt tgaacagcag gcccaggtcg atgtcgccgt
     1501 gggtgacggt gtagaccggt gcacgggcgt tgatgcgcgc caggcgttca cgcagttttt
     1561 ctgcttcgcc tgcgacgtcg gttttagtca gcagaatacg gtcggcgtag ccaacctgtg
     1621 actgggcgat ggtgaactgg ttcatctgct catcggcatg taccgcatcc accagcgcaa
     1681 tcactccgtc cagcaggtaa cgctggcata aaacttcatg ggagaaaaag gtctgaataa
     1741 tcgggccggg atcggccatg ccggtgcatt caatgaccag acggtcgaac tgaatatagc
     1801 ccttgtcgag attgtccagc aggtcgagta gcgcgtcctc cagctcgttg gagcgcgaac
     1861 agcagatgca gccgttggtc agcgttttta tttgcgtggc gcgatcgcca atcagttgat
     1921 catcaacgga gacttcgccg aattcgtttt caatcacggc aatcttgtag ccgtgttgtt
     1981 cgttaagaat atggcgcagc agggtggttt ttcctgcgcc taaaaaaccg gtgagtaggg
     2041 taactgcaat cgggttcatg ctctctccat taacaacagc gcataccgcc tttaccatcg
     2101 ccgccgtagc gcgcgttctg gcgttcgcgg aagaattctt catagctcat gtacggcttg
     2161 tcgggatggt tggtcttcat atgctcgacg tagttgtcgt agtctggaat gccaatcaac
     2221 atcttcgccg cctggccgag atattttttt gcctgtccta agttaccaaa cattttgcct
     2281 tccagatatg cgtaaagccc ggaaggtccg ggctggggat ttgccggatg cggcgtaaac
     2341 gccttatccg gcctacgata gacattttgt aagccggata aggcaaccgg ttagtggtgc
     2401 gaagagatct tcacgccgcc ttccgggatt ggaacgtacg gtgtttcttt gtcagtacgt
     2461 ttgtcgctgt tacgcacctt cagccaggtt ttgaaaccgt agaagatgat gctgtacacc
     2521 acaatcagga acagaatact caggcctgcg ttggtgtagt tgttcacaac gatgtggttc
     2581 atgttggcaa tctgctgcgc cgtcaggtca gtaccgttag caatcttctc tttgtactgg
     2641 cttgccatgt agaagaagcc ttccatctgc gggttggtac tgaacagttt caggcccagc
     2701 gcccaggtgg tgcagataag cagccatact gccgggataa cggtaaccca gatgtattgg
     2761 gtgcgcttca tcttaatcag cacaacggtg cccagtacca gcgctacggc tgccagcatc
     2821 tggttggaga taccgaacag cggccacagg ctcttaacgc cgcccagcgg atcgaccacg
     2881 ccctgataca gcaggtagcc ccacagaccc acacagcccg cagtaccgat gataccggca
     2941 accagagagt cggttttttt caggaacggg atgaagttac ccagcaggtc ttgcagcata
     3001 aagcggccag aacgggtacc cgcatccagc gcggtcagga tgaacagggc ttcgaacaga
     3061 ataccgaagt gataccagaa gcccatgtca gccatcggca gcactttgtg gaacacgtga
     3121 gcgataccta ccgccagcgt cggcgcacca cctgcacggt tcaggacaga aggctcacca
     3181 atgtctttcg cggtttgcag gatctgctct ggcgaaatca cgaagcccca ggagctgacg
     3241 gtcgctgccg cgtgtgcggt aacgtctttc agctgcgcca tgatgatcgg cgcgttctcg
     3301 ccacccattt catgcaggtt aggcatggtg atgccgaggc cagcaggcgg ggtgttcatc
     3361 gcgaagtaaa gacccggttc gatgatggac gcagcaacca gcgccataat cgccacgaag
     3421 gactccatca gcattgcgcc gtagccgatg aaacgcgcgt cggtttcatt agccagcagt
     3481 ttcggcgtcg taccggaaga gatcagcgcg tggaagccag ataccgcacc acaggcgatg
     3541 gtgatgaaca ggaacgggaa cagagcgcct ttccacagcg ggccagtacc gtcaatgtac
     3601 tgggtcatgg caggcatttt cagttccggg ttcagcacca cgatacccag cgccaggccg
     3661 acgataacgc cgattttcag gaaggttgcc agatagtcgc gcggtgcgag gatcagccac
     3721 actggcagca gtgcggaaat aaacgcatag ccaatcagcg cgaaagtaat ggtggtgtct
     3781 ttaaaggtca gtgccggacc ccagtacgga tcgtgagcaa tcacgccacc gaagtagata
     3841 gaggcaacca gcagcacgat accaatgaca gagacttcac ccacacgccc cggacgaata
     3901 aagcgcatgt agatacccat aaacagcgca atcggtacgg ttgagcaaac ggtgaagaca
     3961 ccccacggac tttcggccag ggctttaacc acaatcagcg ccaggacggc gaggatgatg
     4021 atcatgatta agaaacagcc aaacagcgcg atagtccccg gtactggccc catctcttct
     4081 ttgatcatct caccaagaga tgcgccatta cggcgagagg agataaacag caccataaag
     4141 tcctgaaccg caccggccag cactaccccc gccagcagcc acagcgtgcc aggcaggtag
     4201 cccatctgcg cggcgagaac cggacccacc agcggaccag caccggcgat agcggcgaag
     4261 tggtgaccaa acaacacgta acggttggtc ggaacgtagt tcagaccgtc gttgttaata
     4321 accgcaggcg tcgcgcgcgt ggggtcgagt ttcattacct tctgggcgat gtacagactg
     4381 tagtagcgat acgccaccag atacaccgac acagaggcga ccacgatcca cagggcgctg
     4441 acgtgctccc cccgacgtaa tgcaactacc gcgaggcaga atgcaccgat gattccgaga
     4501 atcacccagg gtatgtgctt gaatatcttt ttagtatcca tagtaaaacc tggcatgtat
     4561 tgattaatag ttggccgaag ccgttctagg tttgcgttgc gtttgaggag gtaaattgac
     4621 cgctatcctg cagtgatgtt gccagagtta cgcgcgcgta aagtaaggta aataactgag
     4681 tggttatttt agggacgtaa gcggtcagga atggcggtga gcggcaaagg cgtcgtttaa
     4741 gtgaccataa atatgtgatt tatatcacat atttatattg cgaataattt atgtaaataa
     4801 tggcttttta aattcagagg gtgaataaaa ttactcggcg tcatctccgc gggattttca
     4861 taaagttttt cctttccagg ccgaaaatct tgcatcggtc cacaggaaag agaaaccatg
     4921 ttaaaacgta tcaaaattgt gaccagccta ctgctggttt tggccgtttt tggcctttta
     4981 caactgactt caggcggtct gttctttaat gccttaaaga acgacaaaga aaatttcact
     5041 gttttacaaa ccattcgcca gcagcaatcc acgctgaatg gcagctgggt cgcgttgttg
     5101 cagacgcgta acaccctcaa ccgcgcgggt atccgctaca tgatggatca gaacaatatt
     5161 ggtagcggtt caaccgttgc tgagctgatg cagagtgcca gtatttcgct gaaacaggcg
     5221 gaaaaaaact gggcggatta cgaagcgttg ccgcgtgacc cgcgtcagag caccgccgca
     5281 gcggcagaga tcaaacgtaa ttacgatatt tatcacaatg cgctggcgga gctgatacaa
     5341 ctgttaggtg caggcaaaat caacgagttc tttgatcagc cgacccaggg atatcaggac
     5401 ggtttcgaga agcagtatgt ggcttacatg gagcaaaacg atcggctcta tgatatcgcc
     5461 gtcagcgata acaatgcctc ctacagccag gcgatgtgga ttctggtagg cgtgatgatc
     5521 gtcgtactgg cggtcatctt cgccgtctgg ttcggtatta aagcctcgct ggtcgcgcca
     5581 atgaatcgcc tgattgacag cattcgtcat attgcaggcg gcgatctggt gaaaccgatt
     5641 gaggtggatg gctctaatga gatggggcaa ctggcagaga gtttgcgcca tatgcaggga
     5701 gagctgatgc gtaccgtcgg tgatgtgcgc aacggggcca atgccatcta tagcggtgcc
     5761 agcgaaatcg ccaccggcaa taacgatctc tcttcgcgca ccgagcaaca ggccgcttcg
     5821 ctggaagaga cggcagccag catggagcaa ctgaccgcaa cggtgaaaca gaatgccgaa
     5881 aatgcgcgcc aggccagcca tctggcgtta agtgcttccg aaacggcgca acgcggcggc
     5941 aaagtggtgg ataacgtggt gcagaccatg cgcgatatct ccaccagttc gcagaaaatc
     6001 gccgatatta tcagcgtaat tgacggcatt gccttccaga ccaatattct ggctttgaac
     6061 gcggcggttg aggctgcgcg tgcgggtgag caaggacgcg gttttgcggt ggtcgcggga
     6121 gaagtgcgta atctggccca gcgtagcgct caggctgctc gtgaaattaa aagcctgatt
     6181 gaagactcgg tggggaaagt ggatgttggc tctacgctgg tcgaaagcgc cggggaaaca
     6241 atggcggaga ttgtcagtgc cgtgacccgc gtgacggaca ttatgggcga aattgcttct
     6301 gcttctgatg agcagagccg tggtatcgat caagttggct tagcggttgc tgagatggac
     6361 cgggtaaccc aacaaaacgc cgcgctggtg gaagaatctg ccgccgccgc cgccgcgctg
     6421 gaagagcagg ccagtcgcct gaccgaagca gtggcagtgt tccggattca gcaacagcag
     6481 caacagcagc gtgaagcatc ggctgtggta aaaaccgtga cgccagctac gccgcgcaaa
     6541 atggccgtgg cagatagcga ggagaactgg gaaacgtttt aacgtaatga tgccggatga
     6601 gtatttcatc cggcgttttt attattaaac caggaacagt gaaatcgccg ggatatatgt
     6661 caccagcatt agcattaaca gtagagccgc cagtaggggc agggattgtt taaagaaatt
     6721 tcccaccgaa acttgcgtga tattacatac cagcagcatc acggtaccta ccggtggcgt
     6781 gaatgcgcca atcgacaggt tgaagatcat gatgatgcca aaatgcaccg gatcgatgcc
     6841 aaagtgttcg accaccggtt ttaatagtgg taccaggatc agcaaaatgg cgtttccttc
     6901 cagaaacatc cccagcgtca gcagcaatat gttgataatc aacaggaaca tgtatttgtt
     6961 ttcggtcagc gaagtcacaa aattcgccac tgcgacacca gcatgttcca ttgagaaaat
     7021 ccatcccaat gcggcgctgg ccatgattac cagcatcacc gatgcggttg cgcgtgccgt
     7081 ttcgcccaat gctttgacga catgagaaat ccgcatttca cgatagataa aaaagccaat
     7141 cgccagcacg attagcaccg ctactgcacc tgcttctgtt ggggtgaaaa tgttagcgcg
     7201 aataccaccc accacagcaa aaatgaggaa cagcgcaggc caggcctgtc ccagcgagaa
     7261 caccacctct ttgcccgtag gccagctttc acgcgagggt ttagcgttag ttttgcgggc
     7321 gacaaggtag acggtaaaca tcagtacgac tgcgcatagc aaaccaggta ccagtccggc
     7381 gataaacatt tttccgatag agacgtcggc aaccagaccg taaataatca gggctattcc
     7441 aggcggtatg atcggggtga ctaacgagcc accagcggta atggcggcgg caaacgcgcg
     7501 attgtagcct ttacgctcca tctccggcac catcatgcgc gacagcatcg ccgcgtcagc
     7561 aaggttagag gcgctgacgc cgcccatcat ggtgctgacg agaatgtttg ccacgcccag
     7621 tccgccgcgc attttcccca ccagaacgtt ggcaatgtgc aggatgcgtt cggcaatgcc
     7681 ggtgtagctc attagtgtgc cgagcaagat aaaaaacgga attgccagca atgatgggct
     7741 ttgcgttggc gcgattagtc tttgcacggc aatttcaatc ggtacgctgg agaagaataa
     7801 aaagtaagcg atgatgccaa taaacatcgc cagatagacc cgaatgttga gaaacaaaca
     7861 gacgatcatt agcggaataa tcaataacca actcattgca ttttgtctcc gctatcggat
     7921 tgttttgtat cgtttattaa ccagtagagg gtataaatag ccgttgccag tgcgccgaca
     7981 gggaccgcca gatcgatcca gaaccatgaa accttcagaa tttgtgtcac tttaaactgc
     8041 gccatttcgg ctagtcggta gcccagccag gccatataaa gcagcagtgc gatggagaga
     8101 agggcgacca gtactgcaat aacacgcctg agtttgcctg gaagcatctc catcaggaag
     8161 ggaatggtca ggtgcgccct gtcacgttcc gccaccactc cacctaacat caccacccaa
     8221 atcatcagca ttcccgacat ttcttctgtc cattgaagcg gttggccgat gaaataacgc
     8281 ataaataccg ctccaattgt caccacaacc agaacgaata gcgcaaaggc agcactaaat
     8341 tctgccactt tgccaaaaag acgcatgaaa cctcctgtta gtgcgtgtcc tgcctaagag
     8401 agatgaggca ggacgctgag cattaatgct tattgcagtt gctgttgaat ggtttcgtac
     8461 agacctggtg tccattccgg gaattgagag taaacttcgc gtgctttctt ctggaatggt
     8521 ccagtatcgg gatagatcac ttcgacgccg gctgcctgca tggttttcaa catttcggca
     8581 tcacgctcaa gagtgagttt ctggctatag acacccgctt catagccggt ctggtggatc
     8641 atctccagtt gttctggtga aagggtgcta aagaaggctt caccacctat ccacactgag
     8701 gtatttgtca ggtagttaac cattgtcaga tatcttgcct gctcatacag tttttgccct
     8761 tgcagcactg aaattgggtt ttctacgccg tcaataacgc cctgggttaa tgcgggataa
     8821 acttcgccta atggcatggg agttggtgtt gcccccatcg cctgaatggc ttttatttgc
     8881 atgacgttat taggaacgcg aatttttaaa cctgcgagat cgtcaacggt gcgaatgggt
     8941 tttttcgaaa taatctgtcg agtgccatag agataattat tcattaccac atgaattccc
     9001 tttttcttca gactttcatt tttttgttta aaccagtcgc tttcatatat tttgaacagt
     9061 ttttgcggat cgtcagtcag atacggtcca aacaaaatcc ccaaatcggg ttcataatcg
     9121 gcaagaaaag ccacatcact caaggttatg acattcatgc ccatcatggc ttgctcggtg
     9181 acatcctgtt ttgatcccaa ttgggagctg ggatagagcg caagtgttat ttcgccatta
     9241 ctttttttat ttaataggtc tgcccagtaa cgcatgacca catccagggg ttctccaggg
     9301 ttattttcat aggcaacttt tattgaaata ggttttgcct ggattattga cggtaaaaaa
     9361 agggtactga cagtaattag catcgctatt attttgctca tctttactta ctccatgcta
     9421 aggaaataaa actccaacat tgttgtcggg agtgaaacct tatgaaaaag cagtcattac
     9481 tgaaaagtag tcagcgaata gcagaatggc tgcagatgta tcataaattc gtgcaccggg
     9541 taatactttg ctgcttttaa tcggttttta atgcattttt tgattgtata tcttaaagtt
     9601 atgtaatgca tgacgttaca aggaacaccc tctgatgagt ttagttatcg ctgataagat
     9661 ctcttattcg gttgatgatt tattaaaaat aaaatcatta atttggctgc ggttattttt
     9721 aattgtcaat acagataaa
//
LOCUS       DAZKHK010000160         9724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKHK010000160 DAZKHK010000000
VERSION     DAZKHK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.161"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..614
                     /locus_tag="ACHOS5_004399"
                     /pseudo
     CDS             1..614
                     /locus_tag="ACHOS5_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585556.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            797..873
                     /locus_tag="ACHOS5_004400"
     tRNA            797..873
                     /locus_tag="ACHOS5_004400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:831..833,aa:Arg,seq:tct)
     gene            complement(1152..2429)
                     /locus_tag="ACHOS5_004401"
     CDS             complement(1152..2429)
                     /locus_tag="ACHOS5_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9779120.1"
                     /translation="MVLSDCYSWDNEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSQSTAQVEGAYRLIRNPSVSPQAIAEAGFTATVRACEAHPLLLALEDTTTINFSHS
                     TASDDQGNTTTNPKTHGLLAHSVLMYAPDSALPVGFIEQQRWSRVTDTYGVKHQRKER
                     PCEEKESYRWQQASERMAERLGEIQKRVITVCDREADIWHYLHYKVSHGQRFVVRAAQ
                     NSRLEEAPGKLFELPEVLATAGSHTLNVMQKGGRAARQARMFIRYSEVSIKIATTVAS
                     AADARRIVSHYERRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIKAFIAVRVLG
                     LRQEGISEETQNDSCKKILTPTEWKLLWVKLEGKQLPSQTPTLKWACLKLGRWHDSKR
                     TGRPGWVVMWDGWFRLQDMVEGYPVMKSLDQEI"
     gene            2775..4745
                     /locus_tag="ACHOS5_004402"
     CDS             2775..4745
                     /locus_tag="ACHOS5_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9779121.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMVKDTITIVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGEHAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNSTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYVDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHRGVYAGWRAELSASATHVKKGDPHQAGYTIHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            4764..5555
                     /locus_tag="ACHOS5_004403"
     CDS             4764..5555
                     /locus_tag="ACHOS5_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFP9779122.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRDFFENPRLYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGHELEIIPLS
                     DISRFSENVKLKVLYKTSPLAGAIMELDSVSYLTSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            5819..7018
                     /locus_tag="ACHOS5_004404"
     CDS             5819..7018
                     /locus_tag="ACHOS5_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9779123.1"
                     /translation="MNRIPVIIINGFLGAGKTTLMKNLLTQANRNHLAVSVIVNDMSD
                     LDVDGVLIANTEIVNIANNNFVSITSDSISSQSGIKTVDKAIKNMLRHYLPDVIFVET
                     SGGSHPLPLIKYLQTHERLQLKSLLSLVDTVMLNDDYNGGKALIPAFQENLFHGKRRI
                     ENLLAEQIMFCSTLLLTKKDRLSFDIVTDVAKAIHPLNPYVNVIAVSWGNLKLAELLT
                     LPDYDFNRVGLLIRELEDTITVEEAKISAQNGEIISRVIKDDRPFHPQRLWETYHYFM
                     GMGIYRSKGFFWLPGRPDMALLWNQAAGSINLEFISYWNSGVLADPDNHLTHEERSVL
                     QKKVNKTMGRFGDRRCHLTIIGRSDEVHDFTSALINCFLSEEEIVWWQSGGVFADPWP
                     VNISRLN"
     gene            complement(7125..7310)
                     /locus_tag="ACHOS5_004405"
                     /pseudo
     CDS             complement(7125..7310)
                     /locus_tag="ACHOS5_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            7371..7691
                     /locus_tag="ACHOS5_004406"
                     /pseudo
     CDS             7371..7691
                     /locus_tag="ACHOS5_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7693..7873
                     /locus_tag="ACHOS5_004407"
                     /pseudo
     CDS             7693..7873
                     /locus_tag="ACHOS5_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199731.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            7968..9104
                     /locus_tag="ACHOS5_004408"
     CDS             7968..9104
                     /locus_tag="ACHOS5_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFP9779124.1"
                     /translation="MGKIKYWLIVGFIILFAIFYIAISDRDSTLSRLKSAGENGDVEA
                     QYALGLMYLYGEILDVDYQQAKIWYEKAADQNDPRAQAKLGVMYANGLGVNQDYQQSK
                     LWYEKAAAQNDVDAQFLLGEMYDDGLGVSQDYQHAKMWYEKAAAQNDERAQVNLAVLY
                     AKGNGVEQDYRQAKSWYEKAAAQNSPDAQFALGILYANANGVEQDYQQAKDWYEKAAE
                     QNFANAQFNLGMLYYKGEGVKQNFRQAREWFEKAASQNQPNAQYNLGQIYYYGQGVTQ
                     SYRQAKDWFEKAAEKGHIDAQYNLGVIYENGEGVSQNYQQAKAWYEKAASQNDAQAQF
                     ELGVMNELGQGESIDLKQARHYYERSCNNGLKKGCERLKELLDK"
     gene            9140..9682
                     /locus_tag="ACHOS5_004409"
     CDS             9140..9682
                     /locus_tag="ACHOS5_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779125.1"
                     /translation="MAIPERMSPVKVSSKKIRELAQKAKEILAAIDSGASEEDAFLKD
                     MIDDWNSQVVCPCEFSDFRDYSSWTNANEFTRIAFNLEKFYEDFTWEELVQTISCVCD
                     QKSNESEKIFALLLLEKNFHGNPSDLIFWPDEWFQDPDMLDVELSVEEIACYLMVRSG
                     RQLVDAPEIDLKYQIPQSDE"
BASE COUNT         2720 a         2048 c         2351 g         2605 t
ORIGIN      
        1 cacttccttt tactatggct ttccatggtg ggggcggagc tggtgttgat gatgtggcca
       61 gaagcctgtt ttatgctgtt acagtcatcg ttatattcct tattggatgg ggagcaggta
      121 ttgttttctt tctgggaatt aaacaaaaaa caatatgaag gatttgagtt ttatcagatt
      181 ttttctggca atattgttgc ttcttgttcc tcctgctctg gagtttccag taattcatcg
      241 gcacattacg ccaggtgcac tgactttatg cgtcattaca gccttaataa ttacgctgct
      301 tgccagcttg ggcggcaggc ttgtctcagt taaatgtatt tcggagattt cgtttattcg
      361 tcgtcaccgg attgagtgca tgatggcggg atttatgatt tatttctgga cattctctct
      421 tattgctggt tggtacaagc cccagtttaa gaaggaatat cagacactcc actatgagaa
      481 tgctaggtat tacgttcttg cacgctatga cggtcgtttg gttttatcac aatcgtacag
      541 gagcgggagt aggaagtttg ttataatcaa tggcggacat agactttgag attaatgtag
      601 tcagagtgcg ttaaaattcc cttagaaatc gggaataggt agtactagat acgcaggatt
      661 taatcagtcg tattgagatg gaattgcaga gttaaactgg catggattat tttgtaccca
      721 gcccttaaga acaatggtga gagatagtaa ctcataatcg ccaattcact gttttaaatt
      781 tagtaagggc cgctatccgc cactggctca tcgggaaaga gcatcagcct tctaagttga
      841 ctgtgcgagg ttcgagtcct cggtggcggt ccagtactga tttagctcag taggtaggga
      901 attgccttgt aatcagtagg tcaccagttc gactcttgct gtctcgcccc atattccaat
      961 aggctcgctt tgacggggct tttgtatgtc gcttgcgata tgttacagta ccgttatcag
     1021 tctgtagaaa atacacacag gagtgaaatg ttaaattacc cacaactcag agagcggagt
     1081 gttgtcctgt tagtggggaa tttgttaaaa gaatccagca tggtgaatct ccctgtgcgg
     1141 ctgtctcttg atcagatctc ctgatcaaga gacttcatca ccggataacc ctcaaccata
     1201 tcctgaagtc tgaaccagcc atcccacata actacccaac cagggcggcc tgtgcgtttg
     1261 ctgtcatgcc atcgccccag tttcagacag gcccatttca gtgtcggcgt ctgtgacgga
     1321 agctgttttc cttccagctt aacccacagc agtttccact ctgtcggcgt cagtattttc
     1381 ttacagctgt cattttgtgt ttcttcactg ataccttcct gccgcaggcc cagcacccgt
     1441 accgcgataa acgccttgat aaccaccatg cgctcaaggt tatcccgggt ctgcattcgc
     1501 agtgattcca cacatgtacc accacttttc cacgccttgt ggtattcctc tatcagccag
     1561 cgtcgctcgt aatggctgac gatacgtcgc gcatcggcgg cactcgccac tgttgtcgcg
     1621 atttttatgc tgacttcgct gtacctgatg aacatccggg cctggcgggc tgcccgccct
     1681 cctttttgca tcacattcag cgtgtggctt cccgcggttg ccaggacttc cggcagttcg
     1741 aagagcttgc cgggtgcttc ttccagccgg ctattctgtg cagcacgcac cacgaagcgc
     1801 tgtccgtggc tgactttata atgcaggtaa tgccagatat ccgcttcccg gtcacagaca
     1861 gtgattaccc gtttctgtat ctcccccagc cgttcggcca tacgctccga agcctgctgc
     1921 cagcggtaac tttctttttc ttcacaggga cgttcttttc gctggtgctt aacaccgtag
     1981 gtgtccgtga cccgactcca gcgctgctgt tcgataaaac cgactggcag ggcgctgtca
     2041 ggggcgtaca tcaggacaga gtgagccagc agcccgtgcg tcttcgggtt agtggtggta
     2101 ttcccctggt catcagatgc cgtactgtgg ctgaagttaa tggtggtggt gtcttccagt
     2161 gcgaggagca gcggatgagc ctcacatgcc cttacagtgg cggtaaatcc ggcttcggca
     2221 atggcttgcg gggacacaga cgggttacgt atcaggcggt acgcaccttc aacctgagca
     2281 gtggactggg atgatttcac aatagaaaga cctgcatgct gagcgagaga agaggtcagt
     2341 gacacaaggc gtcgtgtacg acgcggatca ccgagacggg catgtccaga ctgctcgtta
     2401 tcccatgaat aacaatcaga aagtaccata acagacccga agaaaatgaa atataagaaa
     2461 agatcaacga gtgaagaaaa agttcaaaaa atggctgccg gggaggaagg aaagtaccgg
     2521 atggaaagag tcccccctaa agcagactga cagacataac aaatccccgg ggggatttgt
     2581 gtataagaga cagtacttat ctggaggtaa ttgcaatatc tctgtgaact tacacagggt
     2641 gggcttaccg catacactga cacttagcgg atcgacagaa cattattaac agagcatcac
     2701 tgaacgctac ataatcagag ttgcataaat aaaatgttat gatataacat cacaatcaca
     2761 acatttgttt tcgaatgtat atgaatgtaa tcagaactgt catttgtaca ttaattatac
     2821 ttccggtggg actacaggca gcgaccagtc attcttctat ggttaaagat acaatcacca
     2881 ttgtcgcgac aggaaatcag aacacggtat ttgaaacgcc gtcgatggtc agtgtcgtca
     2941 cgaatgacac accgtggagt cagaatgcgg ttacatcggc cggcatgctg aaaggtgttg
     3001 ccggtctcag ccagactggt gcaggacgga ccaatgggca gacctttaat ttacgcggct
     3061 atgacaaaag cggggtactt gttcttgttg acggcgttcg ccaactcagt gacatggcaa
     3121 aaagcagtgg cacttatctg gatccggcac tcgtcaaacg tatcgaagtt gtccgcgggc
     3181 caaactccag tctgtacggc agtggcgggc tgggaggtgt agtggacttc agaactgccg
     3241 atgcagcaga ttttcttccc cccggagaga caaacggttt aagtctgtgg ggaaatatcg
     3301 ccagtggtga ccacagcaca ggctcggggc tcacctggtt tggtaaaact ggaaaaacag
     3361 atgcgctcct ttctgtcatt atgcgtaaaa gaggtaatat ctatcaaagt gatggtgagc
     3421 acgcacctaa caaggaaaaa cctgcagccc tgtttgcgaa aggctctgtc ggtataacag
     3481 acagtaacaa agcaggtgcc agcttgcgtc tctaccggaa taacaccact gaaccgggca
     3541 attccactca gacacatggt gacagcggcc tgcgtgacag aaaaacagta caaaatgacg
     3601 tacagttctg gtaccagtac gctcctgtgg ataacagcct catcaatgta aagtcaacgt
     3661 tatatctcag tgatatcact atcaagacaa acggtcacaa caaaacggca gaatggagaa
     3721 acaacagaac ctccggtgtt aatgttgtca acaggagtca tactctgatt tttccgggag
     3781 cccatcagtt aagttatggc gctgaatatt accgtcagca gcagaagcca gaaggctctg
     3841 ccacactata tccggaagga aacattgact ttacatcatt gtatttccag gatgaaatga
     3901 caatgaaaag ctacccggtt aacattatcg tcggttcccg ctatgaccgg tacaagagct
     3961 tcaatccccg tgccggagaa ctgaaagccg aacgcctgtc cccaagggcg gcgatttcag
     4021 tctcaccgac agactggctg atgatgtacg gctccatatc ctctgcattc cgagcgccca
     4081 caatggcaga aatgtacagg gatgatgtac atttttaccg caagggtaaa cccaattact
     4141 gggttcctaa ccttaatctg aaaccagaaa ataacatcac ccgtgagatt ggcgcaggta
     4201 ttcaactgga tggcctgctt acagacaatg accggctgca gttaaaaggc ggatatttcg
     4261 gaacggatgc cagaaactat attgccacac gcgtggatat gaaacggatg cgttcttatt
     4321 cttataatgt atcccgggcc cgtatctggg gatgggatat gcagggtaat taccagtctg
     4381 attatgttga ctggatgctt tcttataacc ggacggaaag tatggatgcc agcagcaggg
     4441 aatggctggg ctccggcaat cctgacacac ttatcagtga catcagcata cctgttggtc
     4501 atagaggcgt ttatgccgga tggcgtgctg aactttcagc atcagccacg catgtgaaaa
     4561 aaggcgatcc ccatcaggct ggttatacca tacattcctt ttcactgtct tataagcctg
     4621 taagtgttaa aggctttgag gcgtcagtaa ctctggataa tgccttcaac aagcttgcca
     4681 tgaatggcaa aggtgtgccg ctttcaggca gaactgtcag tctttatacc cgttatcagt
     4741 ggtaacctcg caggaaataa atcatgatga aaaatacagg ctatatctta gctctttgtc
     4801 tgacagcatc ggggcatgtc ctggcccatg atgtctggat tacaggtaaa caggcagaga
     4861 acaacgttac cgcagagatt ggttatggtc ataatttccc ctcaaagggg acaattcctg
     4921 acagaaggga tttctttgaa aatccccggc tttataacgg gaaagagaca ataacactga
     4981 agccagcgtc cacggattat gtctataaaa ctgagtctgc aagcaaagat aatggttacg
     5041 ttctgtcaac gtatatgaaa ccgggatact ggtcgagaac ctcgtcagga tggaaaccgg
     5101 tcagccggga gggcagaaat gatgtggctt actgtgaatt tgtcactaaa tatgcaaaat
     5161 cttttattcc tggtgaacag cagatgccag cacaactcta tcagtctcca acagggcatg
     5221 agcttgaaat cattccgtta tccgatataa gtcgtttcag tgaaaatgtg aagctgaaag
     5281 ttctgtataa aacgtccccg ctcgccggag ctatcatgga gcttgactcg gtcagttatc
     5341 tgacatcatc ccgtcatact catgcagttg agcacaaaca tcctgttcat aaagcagaac
     5401 tcacctttgt aactaatgag gatggtatcg tcacagtacc ttctcttcat atcggacagt
     5461 ggctggcgaa agtccaaaat aagaaaagtt ttcaggacaa aagcctgtgt gatgaaactg
     5521 tcgatgtggc aaccttaagc ttctcccgaa attaatgata ctattcctgc cctgtatttt
     5581 acgggcaggg tatatcaacc ggaaatagcc tactgacatc catgtaaaaa aagtcataat
     5641 tattttgttg atgtatctta cctaatacat tgatagctcc agtttttata ccagagcatt
     5701 ctcgttgtcc ctatgtactt gtctgcaata tcactgatat gcaggaataa tattcttggc
     5761 atggagtcag ttccccgtca tcttaggtat cactttaact cacaggtgtc cagtaaatat
     5821 gaacaggatc ccagtaatca ttatcaatgg tttccttggc gcaggaaaaa ccactcttat
     5881 gaaaaacctg ctcacacagg caaacaggaa tcacttagct gtatctgtca ttgtcaatga
     5941 tatgagcgat ctggatgttg atggtgtact tattgcaaat acagaaatcg ttaatattgc
     6001 gaacaataat ttcgtgagta tcacatctga tagtatcagc agccagagtg ggattaagac
     6061 ggtcgacaag gcaattaaaa atatgctcag gcattatttg cctgacgtta tatttgtcga
     6121 aacatccgga ggtagccacc cactgccatt aattaagtat ctgcagactc atgagcgatt
     6181 acagctaaaa tcacttttat cacttgtcga taccgttatg ctcaatgatg actataacgg
     6241 agggaaagca ctgatacctg catttcagga gaatcttttc catggcaagc gcagaataga
     6301 gaatttactg gctgagcaaa tcatgttttg tagcactttg ctgttaacca aaaaggatcg
     6361 actgtcattc gacattgtca ccgatgtggc gaaagcaatc catcctctga atccttacgt
     6421 aaatgtgata gctgtgtcat gggggaacct taaactagca gagttactaa cccttccgga
     6481 ttacgatttt aaccgggtcg gattacttat cagagaactg gaagacacaa tcacagtaga
     6541 agaagcaaaa atttcagccc aaaatggcga aataatttcg cgagtgatta aagatgacag
     6601 gccattccac ccacaaagat tatgggaaac ctatcattat tttatgggaa tgggtattta
     6661 tcgtagtaaa ggctttttct ggctaccagg aagacctgac atggctctgc tctggaacca
     6721 ggccgcagga agtatcaatc tggagtttat cagctactgg aattccggtg tattggcaga
     6781 ccctgataat cacctgaccc acgaagagcg ctctgttttg cagaaaaaag tcaataaaac
     6841 tatggggcgg tttggagaca ggagatgcca tttaacgatt atagggcgtt ctgatgaagt
     6901 ccatgatttt acctccgcct taattaactg ttttctttct gaagaagaaa ttgtctggtg
     6961 gcaatcaggt ggcgttttcg cggatccctg gcccgttaat atatcccggc tgaactgatg
     7021 attaacgcga tattgcaaat atggcatatg aatcgatcgt agtttggcat tcgaatcgat
     7081 catgatttgg gtaaacgttc acggagcgcc gtattgacgt ttatttattg ggtgagtacg
     7141 acgttccatg gcaggagttc atcaacacgg ttagaaggcc attccggcag gacgttgagg
     7201 atatggcgca gatacgcttc cggatcgata ccgttcagtc ggcaggcgcc gatcagcccg
     7261 tacagcagcg cactacgctc tccaccgtga tcgttgccga agaacatgta gatgatatga
     7321 cctttatata ctcactatag cattggtggg atattccaga ggaggtcatc atgtattcgg
     7381 aaaatattgc ggcttacgtc gggctggatg tgcataaagc cccctagcag ttgctattgt
     7441 tgcatctgaa cgacttggca aggttcgtta ttatggtatg gtacgattaa caacgaagcc
     7501 cagtctgtac gtcgattatt tcagaaatgc agggacttta cggaaacatt ctttcctgct
     7561 atgaagctgg cccctgtggt tttgggctat atcatcaact cacagcgatg aatataaaat
     7621 gctaggttat tgcctcttcc cagatagtaa aatcaccgat agacaggatc aaaaatgatc
     7681 atcgggatgc aaccgctacc gtttgcctcg ttctggaaat aaccttgctg atgggggtat
     7741 ggtttggtac gttgtgggga tttgtatgtg gttttcttca tggaaagaca gtggtatgca
     7801 accgggcgtt gcatttgtct caagcatagt cgcaggcatt tgctttggtg ttctcatggc
     7861 tgtgtaccat tagtgtcgaa aggtagttaa caatcttcca gactgggaca gtctggagta
     7921 aagatcgttg tagaggctat atacttttta aatggactgg gggtgagatg ggaaaaataa
     7981 aatattggct aatagtagga tttattatac tttttgcgat tttttacatt gctattagtg
     8041 acagggattc tacgctttct aggttgaaat cagcaggtga aaacggagat gtagaagctc
     8101 agtatgcttt ggggctcatg tatttgtatg gagaaattct ggatgttgat tatcagcagg
     8161 caaagatttg gtatgaaaaa gccgctgacc aaaatgatcc gcgtgcgcag gccaaactcg
     8221 gtgtgatgta tgcaaatggt ctcggggtaa atcaggatta tcagcaatca aaattatggt
     8281 atgaaaaggc ggctgcgcaa aatgatgttg atgcgcaatt tttgcttggg gagatgtatg
     8341 acgatggtct cggggtaagc caagactacc agcatgcaaa gatgtggtat gaaaaagcgg
     8401 ctgctcaaaa tgatgagcgt gctcaggtca atctcgctgt tctatacgca aagggtaatg
     8461 gtgttgaaca ggattatcga caggccaaaa gctggtatga aaaggctgca gctcaaaata
     8521 gtcctgatgc gcagttcgct cttggaattc tgtatgccaa tgctaatggt gtggagcagg
     8581 actatcagca ggcaaaagac tggtatgaga aagcagcaga acaaaatttc gccaatgctc
     8641 agtttaatct tggtatgctc tattacaaag gtgagggtgt taaacaaaac tttcggcaag
     8701 ccagagaatg gtttgaaaaa gccgcatctc aaaatcagcc gaatgcccaa tataatttag
     8761 gtcagattta ttactacggt cagggtgtga ctcagagcta tcgacaggcg aaagactggt
     8821 ttgaaaaagc ggcagagaaa ggtcatatcg atgctcaata taatctcggt gtaatatacg
     8881 aaaatggtga aggtgtgagt cagaactatc aacaggcaaa ggcttggtat gaaaaggctg
     8941 cctcacaaaa tgatgcgcag gcgcagttcg aacttggcgt tatgaatgaa ctgggtcagg
     9001 gtgaaagcat agacctgaaa caagcaagac attactatga gcggtcatgt aataatgggc
     9061 ttaagaaagg ttgtgaacgg ttaaaagagt tattagacaa atagatatta taatatttat
     9121 tatatgaggt tgtgaattaa tggcaatccc tgaacgtatg agccccgtaa aagtcagcag
     9181 caaaaaaata agagaattag cacagaaagc taaagagata cttgcagcta ttgatagtgg
     9241 agcaagtgaa gaagatgctt ttttaaagga catgatcgat gactggaaca gtcaggtagt
     9301 atgtccatgt gagttttcag attttagaga ttattcctct tggacaaatg caaatgaatt
     9361 tacacgaata gcttttaatt tggaaaagtt ctacgaggat tttacttggg aggaactggt
     9421 tcaaacgata agctgtgtat gtgatcaaaa gtcaaacgaa tcggaaaaaa tttttgctct
     9481 tttgttgctt gaaaagaatt ttcatggaaa tccatccgat cttatattct ggcccgatga
     9541 atggtttcag gatccagata tgttagatgt tgaactgagt gttgaagaga ttgcatgcta
     9601 tttgatggtt cgatccggta ggcaactagt tgatgcacct gaaatcgacc tgaaatatca
     9661 aataccccag tctgacgaat aaaaaattaa tataagtgta gataaatagt tatgagcaat
     9721 taca
//
LOCUS       DAZKHK010000161         9687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKHK010000161 DAZKHK010000000
VERSION     DAZKHK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.162"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..1676
                     /gene="aceB"
                     /locus_tag="ACHOS5_004410"
     CDS             75..1676
                     /gene="aceB"
                     /locus_tag="ACHOS5_004410"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP9779126.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGMLPDFISETASIRYADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYSNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGETIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            1706..3010
                     /gene="aceA"
                     /locus_tag="ACHOS5_004411"
     CDS             1706..3010
                     /gene="aceA"
                     /locus_tag="ACHOS5_004411"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP9779127.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            3226..4950
                     /gene="aceK"
                     /locus_tag="ACHOS5_004412"
     CDS             3226..4950
                     /gene="aceK"
                     /locus_tag="ACHOS5_004412"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP9779128.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(4967..5791)
                     /gene="iclR"
                     /locus_tag="ACHOS5_004413"
     CDS             complement(4967..5791)
                     /gene="iclR"
                     /locus_tag="ACHOS5_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP9779129.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSNHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            5991..9674
                     /gene="metH"
                     /locus_tag="ACHOS5_004414"
     CDS             5991..9674
                     /gene="metH"
                     /locus_tag="ACHOS5_004414"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9779130.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVVHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKVSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHS
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         2314 a         2511 c         2710 g         2152 t
ORIGIN      
        1 gctgaacgaa aagcgcacaa cgatccttcg ttcacagtgg ggaagttttc ggatccatga
       61 cgaggagctg cacgatgact gaacaggcaa caacaatcga tgaactggct tttacaaggc
      121 cgtatggcga gcaggagaag caaattctta ctgccgaagc ggtagaattt ctgactgagc
      181 tggtgacgca ttttacgcca caacgcaata aacttctggc agcgcgtatt cagcagcagc
      241 aggatatcga taacggaatg ttgcctgatt ttatttcgga aacagcttcc attcgttatg
      301 ctgactggaa aattcgcggg attcctgcgg acttagaaga tcgtcgcgta gagattaccg
      361 gacctgtaga gcgcaagatg gtgatcaacg cgctcaacgc caatgtgaaa gtctttatgg
      421 ccgatttcga agattcactg gcaccagact ggaacaaagt gatcgatggg caaattaacc
      481 tgcgcgatgc ggttaacggc accatcagct atagcaatga agcaggcaaa atttatcagc
      541 tcaagcccaa tccagcggtt ttgatttgtc gggtacgcgg tctgcacttg ccggaaaaac
      601 atgtcacctg gcgtggtgag acaatccccg gtagcctgtt tgattttgcg ctctatttct
      661 tccacaacta tcaggcactg ttggcaaagg gcagcggtcc ctatttctat ctgccgaaaa
      721 cccagtcctg gcaggaagcg gcctggtgga gcgaagtctt cagctatgca gaagatcgct
      781 ttaatctgcc gcgcggcacc atcaaggcga cgttgctgat tgaaacgctg cccgccgtgt
      841 tccagatgga tgaaatcctt cacgcgctgc gtgaccatat tgttggtctg aactgcggtc
      901 gttgggatta catcttcagt tatatcaaaa cattgaaaaa ctatcccgat cgcgtcctgc
      961 cagacagaca ggcagtgacg atggataaac ccttcctgaa tgcttactca cgcctgttga
     1021 ttaaaacctg ccataaacgc ggtgcttttg cgatgggcgg tatggcggca tttattccga
     1081 gcaaagatga agagcgcaat aaccaggtgc tcaacaaagt aaaagcggat aaatcgctgg
     1141 aagctaataa cggtcacgat ggcacatgga ttgcccaccc aggccttgcg gatacggcaa
     1201 tggcggtatt caacgacatt ctcggctccc gtaaaaatca gcttgaagtg atgcgcgaac
     1261 aagacgcgcc gattactgcc gatcagctgc tggcaccttg tgatggtgaa cgcaccgaag
     1321 aaggtatgcg cgccaacatt cgcgtggctg tgcagtacat cgaagcgtgg atctctggca
     1381 atggctgtgt gccgatttat ggcctgatgg aagatgcggc gacggctgaa atttcccgta
     1441 cctcaatctg gcagtggatc catcatcaaa aaacgttgag caatggcaaa ccggtgacta
     1501 aagccttgtt ccgccagatg ctgggcgaag agatgaaagt cattgccagc gaactgggcg
     1561 aagaacgttt ctcccagggg cgttttgacg atgccgcacg cttgatggaa cagatcacca
     1621 cttccgatga gttaattgat ttcctgaccc tgccaggcta ccgcctgtta gcgtaaacca
     1681 ccacataact atggagcatc tgcacatgaa aacccgtaca caacaaattg aagaattaca
     1741 gaaagagtgg actcaaccgc gttgggaagg cattactcgc ccgtacagcg cggaagatgt
     1801 ggtgaaatta cgcggttcag tcaatccgga atgcaccctg gcgcaactgg gcgcggcgaa
     1861 aatgtggcgt ctgctgcacg gcgagtcgaa aaaaggctac atcaacagcc tcggtgcact
     1921 gactggcggt caggcgctgc aacaggcgaa agcgggtatt gaagcagtct atctgtcggg
     1981 atggcaggta gcggcggacg ctaacctggc ggccagcatg tatccggatc agtcgctcta
     2041 cccggcaaac tcggtgccag ctgtggtgga gcggatcaac aatactttcc gccgtgcgga
     2101 tcagatccaa tggtccgcgg gcattgagcc gggcgatccg cgctatgtcg attacttcct
     2161 gccgatcgtc gccgatgcgg aggccggttt tggcggtgta ctgaatgcct ttgaactgat
     2221 gaaagcgatg attgaagccg gtgcagcggc agttcacttc gaagatcagc tggcatcagt
     2281 gaagaaatgt ggtcacatgg gcggcaaagt tttggtgcca actcaggaag ctattcagaa
     2341 actggtcgcg gcgcgtctgg cagctgatgt gacgggcgtt ccaaccctgc tggttgctcg
     2401 taccgatgcc gatgcggcgg atctgattac ctccgattgc gacccatatg acagcgaatt
     2461 tattactggc gagcgtacca gtgaaggctt cttccgtact catgcgggta ttgagcaagc
     2521 gatcagccgt ggcctggcgt atgcgccata tgctgacctg gtatggtgtg aaacctccac
     2581 gccggatctg gaactggcgc gtcgctttgc acaagccatc cacgcgaaat atccgggcaa
     2641 actgctggct tataactgct cgccgtcgtt caactggcag aaaaacctcg acgacaaaac
     2701 tattgccagc ttccagcagc agctgtcgga tatgggctac aagttccagt tcatcaccct
     2761 ggcaggtatc cacagcatgt ggttcaacat gtttgacctg gcaaacgcct atgcccaggg
     2821 cgagggtatg aagcactatg ttgagaaagt gcagcagccg gaatttgccg ccgcgaaaga
     2881 tggctatacc ttcgtatctc accaacagga agtgggtaca ggttacttcg ataaagtgac
     2941 gaccattatt caaggtggca cgtcttcagt taccgcgctg accggctcca ccgaagaatc
     3001 gcagttctaa gcaaccgata aacgttgctg actgtaggcc ggataaggcg ttcacgccgc
     3061 atccggcaat cagcgcctga tgcgacgctt gcgcgtctta tcatgcctac agccgttgcc
     3121 gaacgtaggc cggataaggc gtctacgccg catccggcaa tcagcgcctg atgtgacgct
     3181 tacgcgtctt atccggcaat tctctgctcc cgacgagggc aacaaatgcc gcgtggcctg
     3241 gaattactga ttgcacaaac cattttgcaa ggtttcgacg ctcagtatgg tcgattcctc
     3301 gaagtgactt ccggggcgca gcagcgtttc gagcaagccg actggcatgc tgtccagcag
     3361 gcgatgaaaa accgtattca tctttacgat catcacgtgg gtctggtcgt ggagcaactg
     3421 cgctgcatta ctaatggcca aagcacggac gcggcatttt tactgcgcgt taaagagcat
     3481 tacacccggc tgttgccaga ttacccgcgc ttcgagattg cggagagctt tttcaactcc
     3541 gtgtactgtc ggttatttga ccaccgctcg cttactcccg agcggctttt tatctttagc
     3601 tcccagccag agcgccgctt tcgtaccatt ccacgcccac tggcgaaaga ctttcacccc
     3661 gatcacggct gggaatctct gctgatgcgc gttatcagcg atctgccgct gcgtctgcgt
     3721 tggcagaata aaagccgtga cattcattac atcgttcgcc atctgacgga aacgctgggg
     3781 acagacaacc tcgcggaaag tcatttacag gtggcgaacg aactgtttta ccgcaataag
     3841 gcagcctggc tggtgggcaa actaataacg ccttccggca cgttgccatt tttattgccg
     3901 atccaccaga cggacgacgg cgagttattt attgatacct gcctgacgac gaccgccgaa
     3961 gcgagcattg tttttggctt tgcgcgttct tattttatgg tttatgcacc gctgcccgca
     4021 gcgctggtcg agtggctacg ggaaattctg ccaggtaaaa ccaccgctga attgtatatg
     4081 gctatcggct gccagaagca cgctaaaacc gaaagctacc gcgaatatct cgtttatcta
     4141 cagggctgta atgagcagtt cattgaagcg ccgggtattc gtggaatggt gatgttggtg
     4201 tttacgttgc cgggttttga tcgggtattt aaagtcatca aagacaggtt cgcgccgcag
     4261 aaagagatgt ctgccgctca cgttcgtgcc tgctatcaac tggtgaaaga gcacgatcgc
     4321 gtgggccgaa tggcggacac ccaggagttt gaaaactttg tgctggagaa gcggcatatt
     4381 tccccggcat taatggaatt actgctccag gaagcagcgg aaaaaatcac cgatctcggc
     4441 gaacaaattg tgattcgcca tctctatatc gaacgacgga tggtgccgct taatatctgg
     4501 cttgagcaag ttgaggggca gcaattgcgt gatgccattg aagagtacgg caacgccatt
     4561 cgccagcttg ccgctgccaa catcttccct ggggatatgt tatttaaaaa ctttggcgtc
     4621 acccgtcacg ggcgcgtggt gttttatgat tacgacgaaa tttgctacat gacggaagtg
     4681 aatttccgcg acatcccgct gccgcgctac ccggaagacg aacttgccag cgaaccgtgg
     4741 tacagcgtct cgccgggcga tgttttcccg gaagagttcc gtcactggtt atgtgccgac
     4801 ccgcgtattg gtccgctatt tgaagagatg cacgccgact tgttccgcgc tgattactgg
     4861 cgcgcactac aaaaccgcat acgtgaaggg catgtggaag atgtttatgc gtatcggcgc
     4921 aggcagcggt ttagcgtccg attcgtataa atcagaggcg aaaaagtcag cgtattccac
     4981 cgtacgccag cgtcacttcc ttcgccgctt taatcaccat cgcgccaaac tcggtcacgc
     5041 ggtcatcggt aatacgtgaa atcggtccgg aaattgaaat tgcggcaaac ggttcgcggt
     5101 gctcatcgaa aatacacgct gcaaggcaac gcagccccag cgcatgttcc tcatcgtcaa
     5161 atgaataacc ccgtttgcgc gtttgggcga gatcttcttt taaatgcaca ggagacacca
     5221 gcgtcgcgtg ggtataggca tgtaaccctt tgcggtgcag cagcttcgtc acctgttctt
     5281 cgctcagttg ggccagaaag gctttacccg caccggaagc gtgcatcggc aatttaccgc
     5341 cgataggcgc ggacatacgc atcaggtgag tacactgtac ctggtcgata ataatcgctt
     5401 cgtgattgct ttgatcaagc accgccatat tgaccgtttc gccagactct tccattagat
     5461 tgcgcaggat agggtgaaca atcgctaaca aattacggct ctggagaaag ctgctaccga
     5521 ccataaaggc gtgtgcgccg attgcccagt gacccagttc gccgacctga cggacaaagc
     5581 cctgttgctg catcgtggtt agcaggcggt gggtcgtgga attgggtaac ccggcttgct
     5641 gtgccagttc cgtgagtgcc acactgccat tggattcggc aatccactcc agtaatttca
     5701 ggccacgcgt taaagactga acctgtccag tcgctggtgc ggtggcaacg gcgggttttc
     5761 tgccgcgttt tgcgggaatg ggtgcgacca tgacagtctc ctttttctgt atcgtggaaa
     5821 tcattttcat ttttattatc agcgaatgca atagttgctg aacagatccg atgagttaat
     5881 gttgaacaaa tctcatgttg cgtggtggtc gcttttacca cagatgcgtt tatgccagta
     5941 tggtttgttg aatttttatt aaatctgggt taagcgtgtc gggagcaagt gtgagcagca
     6001 aagtggaaca actgcgtgcg cagttaaatg aacgtattct ggtgctggac ggcggtatgg
     6061 gcaccatgat ccagagctat cgactgaacg aagccgattt tcgtggtgaa cgctttgccg
     6121 actggccatg cgatctcaaa ggcaacaacg acctgctggt gctcagcaaa ccggaagtga
     6181 tcgccgctat ccacaacgcc tactttgaag cgggcgcgga tatcatcgaa accaacacct
     6241 tcaactccac gaccattgcg atggcggatt accagatgga atccctgtcg gcggaaatca
     6301 actttgcggc ggcgaaactg gcgcgcgcct gtgctgatga gtggaccgcg cgcacgccgg
     6361 agaaaccgcg ctacgttgcc ggtgttctcg gcccgaccaa ccgcacggcg tctatatctc
     6421 cggacgtcaa cgatccggca ttccgcaaca tcacttttga ccaactggtg gcggcttatc
     6481 gagaatccac caaagcgctg gtggaaggtg gcgcggacct gatcctgatt gaaaccgttt
     6541 tcgacaccct taacgccaaa gcggcggtct ttgcggtgaa aacggagttt gaagcgctgg
     6601 gcgttgaact gccgattatg atctccggca ccatcaccga cgcctccggg cgcacgctct
     6661 ccgggcagac cactgaagcg ttttacaact ccctgcgcca cgccgaagcg ctgacctttg
     6721 gcctgaactg tgcgctgggg ccagacgaac tgcgccagta cgtacaggaa ctgtcgcgta
     6781 ttgcggaatg ctacgtcacc gcccacccga acgccgggct gcccaacgcc tttggtgaat
     6841 acgatctcga cgccgacacg atggcaaaac agatacgtga atgggcagaa gcaggctttc
     6901 tcaatatcgt cggcggctgc tgcggcacca cgccacaaca tattgcagcg atgagtcgtg
     6961 cagtagaagg attagcgccg cgcaaactac cggaaattcc cgtagcctgc cgcttgtccg
     7021 gcctggagcc gctgaacatt ggcgaagata gcctgtttgt gaacgtgggt gaacgcacca
     7081 acgtcaccgg ttccgctaag ttcaagcgcc tgatcaaaga agagaaatac agcgaggcgc
     7141 tggatgtggc gcgtcagcag gtggaaaacg gtgcgcagat tatcgatatc aacatggatg
     7201 aagggatgct cgatgccgaa gcggcgatgg tgcgttttct caatctgatt gccggtgaac
     7261 cggatatcgc tcgcgtgccg ataatgatcg actcctcaaa atgggacgtt attgaaaaag
     7321 gtctgaagtg tatccagggc aaaggcattg ttaactctat ctcgatgaaa gagggtgtcg
     7381 acgcctttat ccatcacgcg aaactgttgc gtcgctacgg tgcggcagtc gtggtaatgg
     7441 cttttgacga acagggccag gccgataccc gcgcacggaa aatcgagatt tgccgtcggg
     7501 cgtacaaaat cctcaccgaa gaggttggct tcccggctga agatatcatc ttcgacccga
     7561 acatcttcgc ggtcgcaact ggcattgaag agcacaacaa ttacgcgcag gactttatcg
     7621 gcgcgtgtga agacatcaaa cgtgaactgc cgcacgcact gatttccggc ggcgtttcta
     7681 acgtttcttt ctcattccgt ggcaacgatc cggtgcgcga agccattcac gcggtgttcc
     7741 tctactacgc tattcgcaat ggcatggata tggggatcgt caacgccggg caactggcga
     7801 tttacgacga cctgcctacc gagctacgcg acgcggtgga ggatgtgatc ctcaaccgtc
     7861 gcgacgatgg caccgagcgt ttactcgagc ttgccgagaa atatcgcggc agcaaaaccg
     7921 acgacaccgc taacacccag caggcggagt ggcgctcgtg ggaagtgaat aaacgtctgg
     7981 aatactcgct ggtcaaaggc attaccgagt ttatcgagca ggataccgaa gaagcccgcc
     8041 agcaggctac gcgtccgatt gaagtgattg aaggcccgtt gatggacggc atgaacgtgg
     8101 tcggcgacct gtttggcgag ggcaaaatgt tcctgccgca ggtggtgaaa tcggcgcgcg
     8161 taatgaagca ggcggtggcg tatctcgaac cgtttattga agccagcaaa gagcagggta
     8221 aaaccaacgg caagatggtg atcgccaccg tgaaaggcga tgtccacgac atcggtaaaa
     8281 atatcgttgg tgtggtgctg caatgtaaca actacgaaat tgtcgatctc ggcgtgatgg
     8341 tgcctgcgga aaaaattctc cgtaccgcta aagaagtgaa tgctgatttg attggccttt
     8401 cggggctgat cacgccgtcg ctggacgaga tggttaacgt ggcgaaagag atggagcgtc
     8461 agggcttcac cattccgcta ttgattggcg gcgcgaccac ctcaaaagcg cacacggcgg
     8521 tgaaaattga gcagaactac agcggcccga cggtgtatgt gcagaacgct tcacgcaccg
     8581 ttggtgtggt ggcggcgttg ctttccgaca ctcagcgtga tgatttcgtt gctcgtaccc
     8641 gcaaggagta cgaaaccgtg cgtattcagc acggacgaaa gaaaccacgc acaccaccgg
     8701 tcacgctgga agcggcgcgt gataacgact tcgcttttga ctggcaggct tacacgccgc
     8761 cggtggtgca ccgtctcggt gtgcaggaag tcgaagccag catcgaaacg ctgcgtaatt
     8821 acatcgactg gacgccgttc tttatgacct ggtcgctggc cgggaagtat ccgcgcattc
     8881 tggaagatga agtggtaggt gttgaggcgc agcggctgtt taaagacgcc aacgacatgc
     8941 tggataaatt aagcgccgaa aaaatgctga acccgcgtgg cgtggtgggc ctgttcccgg
     9001 caaaccgtgt gggtgatgac attgaaatct accgtgacga aacgcgtacc catgtgatca
     9061 acgtcagcca ccatctgcgc caacagaccg aaaaaaccgg cttcgctaac tactgtctcg
     9121 ctgacttcgt tgcgccgaag gtttccggca aagcggatta catcggcgca tttgccgtga
     9181 ctggcgggct ggaagaggac gcactggctg atgcctttga agcacagcat gatgattaca
     9241 acaaaatcat ggtgaaagcg cttgccgacc gtctggcgga agcctttgcg gagtatctcc
     9301 atgagcgtgt gcgtaaagtc tactggggct atgcgccgaa cgagaacctc agcaacgaag
     9361 agctgatccg cgaaaactac cagggcatcc gtccggcacc gggctaccct gcctgcccgg
     9421 aacattcgga aaaagccaca atctgggagc tgctggaagt ggaaaaacat accggtatga
     9481 aactcacgga atccttcgcc atgtggccgg gtgcatcggt ttcgggttgg tacttcagtc
     9541 atccagacag caagtattac gcggtggcgc aaattcagcg cgatcaggtt gaagactatg
     9601 cccgccgtaa aggaatgagc gttaccgaag ttgagcgttg gttggcaccg aatctggggt
     9661 atgatgccga gtaagtgata acaccgg
//
LOCUS       DAZKHK010000162         9671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKHK010000162 DAZKHK010000000
VERSION     DAZKHK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.163"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(135..347)
                     /gene="cspA"
                     /locus_tag="ACHOS5_004415"
     CDS             complement(135..347)
                     /gene="cspA"
                     /locus_tag="ACHOS5_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP9779131.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(628..918)
                     /locus_tag="ACHOS5_004416"
     CDS             complement(628..918)
                     /locus_tag="ACHOS5_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP9779132.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1352..2062
                     /locus_tag="ACHOS5_004417"
     CDS             1352..2062
                     /locus_tag="ACHOS5_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP9779133.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2112..3086)
                     /gene="ghrB"
                     /locus_tag="ACHOS5_004418"
     CDS             complement(2112..3086)
                     /gene="ghrB"
                     /locus_tag="ACHOS5_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP9779134.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFGKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3190..3849)
                     /locus_tag="ACHOS5_004419"
     CDS             complement(3190..3849)
                     /locus_tag="ACHOS5_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP9779135.1"
                     /translation="MKKRVYLIAAVVSGTLAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4002..6335
                     /gene="bisC"
                     /locus_tag="ACHOS5_004420"
     CDS             4002..6335
                     /gene="bisC"
                     /locus_tag="ACHOS5_004420"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP9779136.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWQKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKSGVICIHEGAWPDLDLTAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6304..6744)
                     /locus_tag="ACHOS5_004421"
     CDS             complement(6304..6744)
                     /locus_tag="ACHOS5_004421"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9779137.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6741..7304)
                     /gene="tag"
                     /locus_tag="ACHOS5_004422"
     CDS             complement(6741..7304)
                     /gene="tag"
                     /locus_tag="ACHOS5_004422"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9779138.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDIERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7462..8160
                     /locus_tag="ACHOS5_004423"
     CDS             7462..8160
                     /locus_tag="ACHOS5_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9779139.1"
                     /translation="MIIKKSGGRWQLSLLASAVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8389..9591
                     /locus_tag="ACHOS5_004424"
     CDS             8389..9591
                     /locus_tag="ACHOS5_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9779140.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2285 a         2338 c         2498 g         2550 t
ORIGIN      
        1 ttgcacccaa atttattctt cacaaaaata acactagatt ctattacgcg atcgattatt
       61 tatttcctga aaaacaaata aaaaaatccc cgccaaatgg cagggatctt agattctgtg
      121 cttttaagca gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt
      181 tcgatggtga aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata
      241 gcagagaagt gtacgaacac gtctttagag ccatcgtcag gagtgatgaa gccgaagcct
      301 ttgtcagcgt tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt
      361 taataattaa ttgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag
      421 gaatgtgcac tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg
      481 ctttaatggt ctgtacgtca aaccgttgat gtgcattaag ccacgcattg gtgggtgatg
      541 caacaattat ttttcatatt tatgattaat cggttggaaa acggtcctgt tatcaggacc
      601 gtaaacagaa ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt
      661 ggcttgaatc aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc
      721 ccattccttt accatggcga ctgatacgcc caaaacccgg gcaaaatcat cgatttttaa
      781 tcctgtccct tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt
      841 aattttctgc gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat
      901 tggatcttta tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc
      961 gaagctcatt aagaatagtc gggaaaaaca gacacagcgg ggtgtagtca gggcatactc
     1021 tgactatgat tcgattcagc cagtcattta gcgtgactta tagccaccta acgatctgat
     1081 aaatgccgat atatctctta ccactcccgg tttgtaaaga tttcgaaaat ctaacgttct
     1141 gcgaaggcgt aaatctctgg ctacaaaaaa ggcaacggca atatcatttt ttaacaatga
     1201 aacaacaaat taaccttgtt tcattttctc catgatttta ccaggaagat tacgtagaaa
     1261 gggtatcctg caagcctgtc ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg
     1321 tgcgcttttt ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg
     1381 gtttgcgcct cttgcggcgt tattaatggt agttagcctg agtgggtgtt ttgataaaga
     1441 aggcgatcag cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc gtagcggtga
     1501 acgtctacca accctgactg ccgatcagaa aaaacagttt ggtccttttg tctctgatta
     1561 cgcgattctg tatggttatt ctcagcaggt gaatcaggcg atggattccg gtctgcgccc
     1621 ggttgtagac agcgttaacg caattcgcgt accgcaggat tacgttacgc aaagcggtcc
     1681 gctgcgtgaa atgaacggtt cgctgggtgt gctggcgcaa cagctgcaaa atgcgaagtt
     1741 gcaggccgat gctgcgcact ctgcgttaaa acagagtgat gacctgaaac cggtctttga
     1801 tcaggcattc accaaagtgg taacgacgcc agctgatgca ttgcaaccgt tgattccggc
     1861 ggcacaaact ttcacgcaac aactggtgat ggttggagac tacattgctc agcaaggtac
     1921 tcaggtgagc tttgtcgcaa atggcatcca gttcccgact tcacagcagg caagtgaata
     1981 taacaaactg attgcgccat taccggcaca acatcaggcg tttaatcagg cctggactac
     2041 agcagttact gcaacccaat aaagagtaaa agcccgagcg attatcgggc tttactccac
     2101 gcagtcgcgg cttagtccgc gacgtgcgga ttcacacagt tcttctcaac ctttccttgt
     2161 aacgcatcaa tcaaattatc cacggcacag gcggccatgc cataacgcgt ctcatgagtg
     2221 gcagatccaa tatgcggtac tgcgacgacg ttggccattg agagcaacgg cgaatctacg
     2281 gacagtggct cttgttcgaa gacatccagc ccggcggcgt gaatttctcc tttctgcaat
     2341 gcagcaatca gtgcattttc atcaaccacc gggccacgtc cggcattaat gaaaatggcg
     2401 gaggatttca ttttgccgaa ttgttctgcg ccaaatagat gatacgtctc atcagttaac
     2461 ggcaggatca ggcaaacgaa atctgactct tgtaacagtg tatccaaatc gcagtagcgg
     2521 gcgttgaagc gttcttctgc ttctttatgg tggcggcgcg cgttatagag gatgggcatg
     2581 ttgaaaccaa agtgcgcacg ttgtgccagc gccataccga tccgtcccat cccgacaatg
     2641 cccagtgttt tatggtgaac gtcagtgccg taccagtccg ggcctatgct cgcggtccat
     2701 tcgcctgctt ttacccgttc tgctacctcc acaacccgac gagcggtaga caacaccagc
     2761 gccatcagcg tatcggcgac ggtttctgtt aatacggttg gtgtgtgcat cagcagaatt
     2821 tttcgggcgg taagcgcatc gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg
     2881 gcacgcagtt tcggcatttt ttccagcaat gcggcatcaa cattctcgtt tgaacccagc
     2941 aagccttcag cttcggcaaa aattgctgca ttttgttcga cggtttgtgg gctgaggttt
     3001 gccacctggt gaacggtgaa atgttcttgc aggcgttgca gtaaatcatc aggtaaggct
     3061 ttgtagagga taacggacgg cttcatgctt ctctccattc actgataagg cgatcacctt
     3121 agcctgaaat atgatgaagg aaaactgaag aatgccaggt gatttctgca tcacctgaca
     3181 tgaaagggat tacagtgggc ttaaggtaat ttctacacgg cggttttgcg ccttaccttc
     3241 tgcggtgctg ttgctggcga ttgggttagc cgggccaagg ccttgagtac ggatgcggct
     3301 ggcgtccacg ccctgggtga tcaacgcgct ggcaacggaa tccgcacgct gctgggagag
     3361 acgcatgttc aggtcgtgac cccccgtgct gtcggtataa ccaatcacgt taaccgccgt
     3421 tttcggatac tctttcagta ccattgccac gccggtcagg gtgttagcgc ccgccggttt
     3481 cagggttgcg ctgctgctgt cgaaggtcac attgttcggc atattgagga taatgttatc
     3541 cccgctgcgg gttacgctaa caccagtgcc gcgcattttg tcacgcagct tcgcttcctg
     3601 cacatccatg taataaccaa cgccgccgcc cagagctgcg cctgctgctg cgccaatgag
     3661 cgcgccttta ccgcgatctt tcttcgaaga agagagcgca ccaatacccg cgccaacgag
     3721 agagcccaga cctgcgccga tagcagattt acctgcttcg cgttcgccgg tgtaagggtt
     3781 agttgtacag ccagataccg ccagagtgcc actcactacg gcggcaataa gataaacacg
     3841 tttcttcatt gttaatcctt aataaccttt ttattctttg ccacgggttc cgtggcggga
     3901 gattatgccg cgtgagcatg aagattattc ctgggaatac tcggaaattt gtaagtaata
     3961 tttaactgct caatacatct aacctttcag gagtcttcgg tttggccaac tcatcctcac
     4021 gatattccgt tctgactgcc gcccactggg ggcctatgct ggttgaaacc gacggcgaaa
     4081 ccgtgtttag ctcgcgtggc gcgttagcca caggaatgga aaactccttg cagagcgcgg
     4141 ttcgcgacca ggttcacagc aatacgcggg tgcgatttcc aatggtgcgc aaaggctttc
     4201 ttgcgtcacc ggaaaatccg caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt
     4261 gggatgaggc gctggagctt attcaccatc aacataaacg tattcgtgag gcttatggtc
     4321 cggcatcgat ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg cataaggcct
     4381 cgacattatt acaacgctat atggcgctgg caggcggtta tactgggcat ctgggggatt
     4441 attcgaccgg cgcggcacag gcgatcatgc cgtatgtcgt gggtggcagc gaagtttatc
     4501 aacagcaaac cagttggccg ctggtgctgg aacatagcga tgtcgtggtg ctgtggagtg
     4561 ctaacccact caatacgctg aaaattgcgt ggaatgcatc cgatgagcag gggctttctt
     4621 acttttccgc actgcgtgac agcgggaaaa agctgatctg cattgatcca atgcgatcgg
     4681 aaaccatcga tttctttggc gataaaatgg agtgggtggc accacacatg ggcaccgatg
     4741 ttgcgttgat gctggggatc gcccatacgc tggtggaaaa tggttggcac gacgaagcgt
     4801 ttctggcgcg ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg
     4861 acggtatagc gaaaaacgcc gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa
     4921 tccgcgagct ggcggctatt ttccaccaaa atacaaccat gctgatggca ggctggggaa
     4981 tgcagcgcca acagtttggt gagcaaaaac actggatgct cgtcacgctg gcggcaatgt
     5041 tggggcaaat cggcacaccc ggcggcggtt ttggtctttc ttaccatttt gccaatggtg
     5101 gtaaccccac gcgccgcgct gcggtgctct cttccatgca gggcagcttg ccgggtggca
     5161 ccgatgcggt agataaaatc cctgtcgccc gcattgttga agcactggaa aaccctggtg
     5221 gcgcatatca acataacggt atggaccgac atttcccgga tattcgtttt atctggtggg
     5281 cgggtggtgc caactttact catcatcagg ataccaatcg cctgatccgt gcctggcaaa
     5341 aaccggagct ggtggtgatc tccgaatgct tctggacggc tgcggcgaaa cacgcggata
     5401 tcgttctgcc tgcgaccacc tcttttgagc gtaatgatct caccatgacc ggtgattaca
     5461 gtaatcagca tctggtgccg atgaagcaag tggtgccgcc acgcgatgaa gcgcgtaatg
     5521 attttgatgt ttttgccgac ttgagtgaac gttggcagaa gggcggttat gcacgtttta
     5581 cggaaggaaa aagtgagctg caatggctgg aaacgtttta taacgttgcc cggcagcgcg
     5641 gggcaagcca gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa
     5701 ttgagatgcc ggaaaacccg gacagtgagc ggtttattcg cttcgccgat tttcgccgcg
     5761 atccgcaggc gcatccatta aaaaccgcca gcggtaagat tgaaatcttc tcgcagcgta
     5821 ttgccgatta cgcttatccg gattgcccgg ggcatccaat gtggctggcg ccggacgaat
     5881 ggcagggcaa tgccgaaccg gaacagttgc aggtactttc tgctcatccg gcgcatcgtc
     5941 tgcacagcca gctgaattac agttctctgc gcgaattgta cgcggtggca aatcgtgagc
     6001 ctgtcaccat tcatcctgac gatgcccaga cgcgcggcat acaagatggc gatactgttc
     6061 ggctgtggaa ctcacgcggg caaattcttg ccggcgcggt cattagcgag ggaattaaat
     6121 ctggcgtgat ttgtattcac gaaggcgcat ggccggattt ggatttaact gctggcggca
     6181 tttgtaaaaa cggcgcagtg aacgtgctga ccaaagatct ccccagctcg cggctgggga
     6241 atggctgtgc gggtaatacg gcgctggcat ggctggaaaa atacaacggt ccggaactga
     6301 cacttacagc gtttgaacca ccggccagct cataatccag gtgggtagtt gggtttcatc
     6361 ctgccatgcg caatcgacaa tgtgaaaacc ctgcgcatgg tagaaatcta tcgccggttg
     6421 atttttttga taaacctcca gcatcagatg gggatggcgc tgctgcacat actgcatcag
     6481 cgccttacca ataccgcgtc tgacggcctt cggtgcgaca aacattgctg cgaggaatcg
     6541 gccttccata atgctgacaa aaccgagaag cttaccgtct tcttcccaga cccagttttg
     6601 cgcgttggca agatacgcat cccgcaccag cggaatgcag tcacgccagt aactcgattt
     6661 tataaaggga tgcccccagg ttgtactttc cagccacagt tcgaggagcg cggggagttc
     6721 tgaacgttgc gcttcccgaa tcatggttta tttcccggat ggcaacagca gccaaccaca
     6781 tgatcattca ccagcccaca tgcctgcata aaggagtaac agattgtggt accgacaaac
     6841 ttaaaaccac gtttttttag tgctttagat agggcgtcgg aggcgggtgt agatgtgggg
     6901 atttcgctca acgttgtggc ttgtgtcacc tgtggttgat gattgacaaa cgaccagaca
     6961 aagtcggcaa acggttcgcc gttctgttcc atttgcaggt acgcccgcgc attaccaata
     7021 attgcctgaa ttttcccacg atggcggata atcccggcgt cctgtaccag tctttcgata
     7081 tcctcttcct gcattgcggc aactttcacc ggatcgaact gatgaaagca tgcgcgatag
     7141 ttttcgcgtt ttttgaggac ggtgatccac gataacccag cctgctgccc ttccaggcag
     7201 atcatttcga acagtttttt actgtcagtt tcaggcacgc cccattcatt atcatggtag
     7261 gcaatataaa gcgggtcctg actcacccag ccgcaacgtt ccatactttc cctcgctatg
     7321 tacccgatcg taaaaatttc agccgctttt cttgacgcat cggtgaaaaa gtcaatattt
     7381 aatacagggc tactatgccc gatatccttc atacaatgac aataatattg ccgaattcgg
     7441 ctcttccctg gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc
     7501 ctgcttgcga gcgcggtaat cagtgccttt tttctcaaca cagcttacgc ctggcaacaa
     7561 gaatatatcg ttgatacgca acccggacat tccacagagc gttacacctg ggatagcgat
     7621 catcaacctg attacaacga tattttgtcg caacgtattc aaagtagcca aagggcgctg
     7681 ggactggaag tcaatctggc ggaagaaacc cctgtggatg tgaccagcag tatgagtatg
     7741 ggctggaatt ttcctttata tgagcaggtt acaaccggcc cggtcgcggc attacattac
     7801 gatggcacaa ctacctcgat gtataacgag tttggcgaca gtactaccac gctggccgat
     7861 ccgttatggc atgccagcgt gagtacctta ggctggcgtg ttgactcccg gcttggcgat
     7921 cttcgaccct gggcgcaaat cagctataac cagcaatttg gcgagaatat ctggaaggcg
     7981 caatcaggcc tgagccggat gacggcgaca aaccagaacg gcaactggct ggatgtcacc
     8041 gtaggcgctg atatgttgct caatcaaaat attgctgcct atgctgcgct atctcaggca
     8101 gaaaatacca ctaacaatag cgactatctg tatacgatgg gggttagtgc cagattttaa
     8161 cgttatagat acaattgaaa cccataaata acaactaatg tggtgatagt ggggactaac
     8221 ttactaaata ataaatttgg cgaataattg tcgagtcatt cattcctgaa ctaatgcatt
     8281 tcattccgtt ctgatggcat ttcatgccgt ttttccccag gcataaagcg cacttcgtta
     8341 tggttgtcag cagagatttt tcctttttat tactgcagga atactgccat gacaccttca
     8401 aattatcagc gtactcgctg gctgacactc atcggtacta tcattaccca gtttgctctg
     8461 gggtcggttt atacctggag tctgtttaac ggtgcgcttt ctgccaagct gggggctcct
     8521 gtaagccagg ttgctttctc tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct
     8581 gttgcgggca aattgcagga acgttttggt gttaaacgcg tcactatggc ttccggcatt
     8641 ttgctgggat taggtttctt cctgacagca cattccaaca acctgatgat gctgtggtta
     8701 agcgccggtg tgttggttgg tctggcggat ggtgcgggtt acctgttgac gctctctaac
     8761 tgcgtgaagt ggtttccgga gcgtaaggga cttatctctg cttttgctat cggttcttat
     8821 ggtctgggca gcctgggttt taaatttatc gacacgcacc tgctcgaaac ggtgggtctg
     8881 gaaaaaacct ttgttatttg gggcgcgatt gtactggtga tgattgtctt tggcgcaacg
     8941 ttaatgaaag atgcgccgaa gcaggaagtg aaaaccagca atggtgtggt ggagaaggac
     9001 tacaccctgg cagagtcgat gcgtaaaccg cagtactgga tgttagcggt tatgttcctg
     9061 actgcgtgca tgagtggtct gtatgtgatt ggtgtagcga aagatatcgc tcaaagtctg
     9121 gcgcatcttg atgcaatttc cgcagccaat gctgtgacgg ttatttccat cgccaacctt
     9181 tctggtcgtc tggtgctggg cattctgtct gacaaaatcg cccgtatccg tgttatcacc
     9241 attggtcagg tgattgcgct ggtgggtatg gcggccctgc tgtttgcacc attgaatgca
     9301 gtgacgttct ttgcagcgat tgcctgtgtg gcatttaact ttggtggcac gattacggtg
     9361 ttcccgtcac tggtcagtga gttcttcggc ctcaataacc tggcgaaaaa ctacggcgtc
     9421 atttacctcg gtttcggtat cggcagcatt tgtggttcga ttatcgcttc actgtttggc
     9481 ggattctatg tgactttcta cgtgattttc gccctgctga ttctgtcact ggcgctttct
     9541 acgaccattc gccagccaga gcagaaaatg ttgcgtgaag cacatattta gaaatatgtt
     9601 gctgctgaaa taaatacgat gagggagact tcgaagtctc ccttttttta tggttgcatg
     9661 ttaaaagaac a
//
LOCUS       DAZKHK010000163         9612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKHK010000163 DAZKHK010000000
VERSION     DAZKHK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.164"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..1054
                     /gene="glaH"
                     /locus_tag="ACHOS5_004425"
     CDS             77..1054
                     /gene="glaH"
                     /locus_tag="ACHOS5_004425"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP9779141.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTNR
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDLFFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1074..2342
                     /gene="lhgO"
                     /locus_tag="ACHOS5_004426"
     CDS             1074..2342
                     /gene="lhgO"
                     /locus_tag="ACHOS5_004426"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP9779142.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKVFCDQNGIRYDNCGKMLVATSELEMERM
                     NALWKRTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPPGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2365..3813
                     /gene="gabD"
                     /locus_tag="ACHOS5_004427"
     CDS             2365..3813
                     /gene="gabD"
                     /locus_tag="ACHOS5_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9779143.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3827..5107
                     /gene="gabT"
                     /locus_tag="ACHOS5_004428"
     CDS             3827..5107
                     /gene="gabT"
                     /locus_tag="ACHOS5_004428"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP9779144.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5344..6744
                     /gene="gabP"
                     /locus_tag="ACHOS5_004429"
     CDS             5344..6744
                     /gene="gabP"
                     /locus_tag="ACHOS5_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP9779145.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVLSTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            6765..7427
                     /gene="csiR"
                     /locus_tag="ACHOS5_004430"
     CDS             6765..7427
                     /gene="csiR"
                     /locus_tag="ACHOS5_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP9779146.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVTGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHNQHQILTEAVLARDAARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7428..7877)
                     /gene="kbp"
                     /locus_tag="ACHOS5_004431"
     CDS             complement(7428..7877)
                     /gene="kbp"
                     /locus_tag="ACHOS5_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP9779147.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTAIPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7961..8119)
                     /gene="yqaE"
                     /locus_tag="ACHOS5_004432"
     CDS             complement(7961..8119)
                     /gene="yqaE"
                     /locus_tag="ACHOS5_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9779148.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8302..8601
                     /gene="ygaV"
                     /locus_tag="ACHOS5_004433"
     CDS             8302..8601
                     /gene="ygaV"
                     /locus_tag="ACHOS5_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9779149.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRGEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8611..9135
                     /gene="ygaP"
                     /locus_tag="ACHOS5_004434"
     CDS             8611..9135
                     /gene="ygaP"
                     /locus_tag="ACHOS5_004434"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP9779150.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9182..9586)
                     /gene="stpA"
                     /locus_tag="ACHOS5_004435"
     CDS             complement(9182..9586)
                     /gene="stpA"
                     /locus_tag="ACHOS5_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP9779151.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2194 a         2489 c         2649 g         2280 t
ORIGIN      
        1 tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat
       61 aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg
      121 ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact
      181 caccttcacc gaacagacga ccaatcggtt cctcgaacag gttgccgagt ggcccgtgca
      241 ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggc aaaattctcg acgatctgtg
      301 tgcgaatcag ctgcaaccgc tgctgttgaa gaccctgtta aaccgcgctg aaggtgcgct
      361 gttgatcaat gcggtgggta tcgatgatgt cgcgcaggcg gatgagatgg tgaagctggc
      421 gacggcggtg gcgcatctga ttggtcgctc caattttgac gccatgagcg gtcagtatta
      481 cgcgcgtttc gtggtgaaaa atgttgataa ctcagacagc tatcttcgtc agccgcaccg
      541 cgtaatggag ctgcacaacg acggcactta cgtcgaagag atcactgatt acgtgctgat
      601 gatgaaaatc gacgagcaga acatgcaggg cggtaactcg ctgctgctgc atctcgatga
      661 ctgggaacat ctggacctct ttttccgcca tccgctggcg cgccgtccga tgcgctttgc
      721 cgcgccgccg agcaaaaacg tcagcaaaga tgtcttccat ccggtgttcg atgtcgatca
      781 gcagggccgc ccggtgatgc gctatatcga ccagtttgtc cagccgaaag acttcgaaga
      841 aggcgtgtgg ttgagcgagc tttccgatgc cattgaaacc agtaaaggca ttctttctgt
      901 acccgttccc gttggcaaat tcctgttgat taacaacctg ttctggctgc acggtcgcga
      961 ccgctttact ccgcacccgg atctgcgccg tgaactgatg cgtcagcgtg gctatttcgc
     1021 ttacgccact caccactacc agacgcatca gtaagcgcaa aggaattgag cggatgtatg
     1081 attttgtgat tattggcggc ggcatcatcg gcatgtcgac cgccatgcaa ctgattgatg
     1141 tttatccgga tgcccgcatt gcgttgctgg aaaaagagtc cggcccggcc tgtcaccaga
     1201 cgggccacaa cagcggcgtg atccatgccg gagtctatta cacgcccggc agcctgaagg
     1261 cgcagttttg cctggcgggg aaccgtgcca ctaaagtctt ttgcgatcaa aacggcattc
     1321 gctacgacaa ctgcggcaag atgctggtcg ccacgtctga actcgaaatg gaacggatga
     1381 atgcgttatg gaaacgcacg gcggcaaacg gaatcgagcg cgagtggtta aacgccgagg
     1441 agctgcgcga gcgtgaaccg aatatcaccg ggcttggcgg catttttgtg ccgtccagcg
     1501 gcattgtcag ctaccgcgaa gtgacggcgg cgatggcaaa aatcttccag gccagaggtg
     1561 gtgagattat ttataacgcc gaagtcagcg ccctcagtga gcataaaaac ggcgtggtga
     1621 tacgtacccg tcagggcggt gaatatgaag catcgacgct gattagctgt tccgggctga
     1681 tggctgaccg gctggtgaaa atgctcggcc tcgaaccggg ctttattatc tgcccgttcc
     1741 gcggcgagta tttccgcctt gcgccggagc ataaccagat tgttaaccac ctgatttacc
     1801 ccattcccga cccggcaatg ccatttttgg gcgtgcatct cacccgcatg atcgacggca
     1861 gcgtgaccgt cgggccaaac gcggtgctgg ctttcaaacg cgaaggctat cgtaagcgcg
     1921 acttctcatt tagcgacacg ctggaaattt tgggctcgtc ggggattcgc cgagtgctgc
     1981 aaaaccatct acgctcagga ctgggcgaga tgaaaaactc gctgtgcaaa agcggctatc
     2041 tgcggctggt gcaaaagtat tgtccccggc tttcgttaag cgatctccag ccctggcccc
     2101 ccggtgtgcg ggcgcaggca gtatcgccgg acggcaagct gattgacgat tttctgtttg
     2161 tcaccacccc gcgcacgatc cacacctgta atgcgccctc cccggcagcg acatcagcaa
     2221 ttcctattgg tgcgcatatt gtcagcaagg tacaaacgct gttggcaagc cagagtaacc
     2281 ccggacgcac gctgcgagcg gcacgtagtg tggatgcctt acacgccgca tttaatcaat
     2341 aacctttgaa aacaggatgt agcgatgaaa cttaacgaca gtaacttatt ccgccagcag
     2401 gcgttgatta acggggaatg gctggacgcc aacaatggcg aggtcatcga cgtcaccaat
     2461 ccggcgaacg gcgacaagct gggtagcgtg ccgaaaatgg gcgctgatga aacccgcgcc
     2521 gctatcgacg ccgccaaccg cgccctgccc gcctggcgtg cgctcactgc caaagaacgc
     2581 gccaacattc tgcgcaactg gttcaattta atgatggagc atcaggacga tttagcgcgc
     2641 ctgatgaccc tcgaacaggg caaaccactg gctgaagcga aaggcgaaat cagctacgcc
     2701 gcctcattta ttgagtggtt tgcagaagaa ggcaaacgta tttatggcga caccattccc
     2761 ggtcatcagg ccgataaacg cctgattgtt atcaagcagc cgattggcgt caccgcggct
     2821 atcacgccgt ggaacttccc ggcggcgatg attacccgta aagccggtcc ggcgctggcg
     2881 gcaggctgca cgatggtgct gaagcccgcc agtcagacgc cgttctctgc gctggcgctg
     2941 gcagaactgg cgattcgcgc gggcgttccg gctggcgtgt ttaacgtagt caccggttcg
     3001 gcgggcgcgg tcggtaacga actgaccagc aacccgctgg tgcgcaagct gtcgtttacc
     3061 ggctcgaccg aaattggccg tcagttaatg gaacagtgcg cgaaagacat caaaaaagtg
     3121 tcgctggagc tgggcggcaa cgcaccgttt attgtctttg acgatgccga tctcgataaa
     3181 gccgtggaag gcgcgctggc ctcgaaattc cgtaacgccg ggcaaacctg cgtctgcgcc
     3241 aaccgcctgt atgtgcagaa cggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg
     3301 gtgagcaaac tgcacatcgg caacgggctg gagaaaggcg tcaccatcgg gccgctgatc
     3361 gatgaaaaag cggtagcaaa agtggaagag catattgccg atgcgctgga gaaaggcgcg
     3421 cgcgtggttt gcggcggtaa agcacatgaa cgcggcggta acttcttcca gccgaccatt
     3481 ctggtggacg ttccggctaa cgccaaagtg tcgaaagaag agacgtttgg ccccctcgcc
     3541 ccgctgttcc gttttaaaga tgaagccgat gtgattgcgc aagccaatga caccgaattt
     3601 ggacttgcag cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagca
     3661 ctggagtacg gcatcatcgg catcaacacc ggcattattt ccaatgaagt ggccccgttt
     3721 ggcggcatta aagcctcggg tctgggtcgt gaaggttcga agtatggcat cgaagattac
     3781 ttagaaatca aatatatgtg catcggtctt taactggaga atgcgaatga gcagcaataa
     3841 agagttaatg cagcgccgca gtcaggcgat tccccgtggc gttgggcaaa ttcacccgat
     3901 tttcgctgac cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg agtatcttga
     3961 tttcgcgggc gggattgcgg tgctcaatac cgggcacctg catccgaaag tggttgcggc
     4021 ggtggaagcg cagttgaaaa aactgtcgca cacctgcttc caggtgctgg cttacgagcc
     4081 gtatcttgag ctgtgcgaga ttatgaatca gaaggtgccg ggcgattttg ccaagaaaac
     4141 tctgctggtt acgaccggtt ccgaagcggt ggaaaatgcg gtgaaaatcg cccgcgccgc
     4201 cactaaacgt agcggtacta tcgcctttag cggtgcgtat cacgggcgca cgcattacac
     4261 actggcgctg accggcaagg tgaatccata ctctgcgggc atggggctga tgccgggtca
     4321 tgtttatcgc gcgctttatc cttgcccgct gcacggcata agcgaggatg acgctatcgc
     4381 cagcatccac cggatcttca aaaatgacgc cgcgccggaa gatatcgccg ccatcgtgat
     4441 tgagcccgtt cagggcgaag gcggtttcta cgccgcgacg ccagccttta tgcagcgttt
     4501 acgcgcgctg tgtgacgagc acgggatcat gctgattgcc gatgaagtgc agagcggcgc
     4561 agggcgtacc ggcacgctgt ttgcgatgga gcaaatgggc gtggcaccag atctcaccac
     4621 ctttgcgaaa tcgatcgcag gcggtttccc gctggcgggc gtcaccgggc gcgcggaagt
     4681 gatggacgcc gtcgcaccgg gcggactggg tggcacctat gccggtaacc cgattgcctg
     4741 cgtagcggcg ctggaagtgt tgaaggtgtt cgagcaggaa aatctgctgc aaaaagccaa
     4801 cgttctgggg cagaagctga aagacggatt gctggcgatc gccgaaaaac acccggagat
     4861 cggcgacgta cgcgggctgg gggcgatgat cgccatcgag ctgtttgaag atggcgatca
     4921 cagcaaaccg gacgcaaaac tcaccgccga gatcgtggca cgcgcccgcg ataagggtct
     4981 gattcttctc tcctgcggcc cgtattacaa cgtgctgcgc atccttgtac cgctcaccat
     5041 tgaagacgct cagatccgtc agggtctgga gatcatcagc cagtgttttg ctgaggcgaa
     5101 gcagtagcac ggcaccatgc cgggggcgac gctttgcagc ttgtccggac tacgagaatc
     5161 caggtcggat aagacgttta cgccgcatcc gacaatctgt acgtgaacag gaataaatct
     5221 atgtcggccg gataaggcgg agccgctctc cggcaaaaag aaccaataac aattaaacgc
     5281 gtgacccgac gcgggaaatg tcggggcgct ctcccaagtg acacactttc gagaggattc
     5341 aggatggggc aatcatcgca accacatgag ttaggcggcg ggctgaagtc acgccacgtc
     5401 accatgttgt ctattgccgg tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc
     5461 atcgccgaag cgggcccggc ggtattactg gcctatctgt tcgccggatt actggtggtt
     5521 atgattatgc ggatgttggc ggaaatggcg gttgccacgc ccgataccgg ttcattttcc
     5581 acctatgccg ataaagccat tggccgctgg gcgggctata ccatcggctg gttgtactgg
     5641 tggttttggg tactggttat cccacttgaa gccaacatcg ccgccatgat cctgcactcg
     5701 tgggttccag gcattcccat ctggttattt tccctcgtca ttaccctcgc cttaaccggc
     5761 agtaatttat taagcgttaa aaactacggc gaatttgagt tctggctggc gctgtgtaaa
     5821 gtcatcgcta tcctggcctt tattttcctt ggtgcagtcg caattagcgg tttttacccg
     5881 tatgccgacg tgagcggtat ttcaagattg tgggatagcg gcggctttat gcccaacggc
     5941 ttcggtgcgg tattaagcgc gatgttgatc accatgttct cgtttatggg cgcagaaatt
     6001 gtcaccattg ccgccgcgga atccgacacg ccggaaaaac atattgtccg cgccaccaac
     6061 tcggttatct ggcgtatctc tattttctat ttgtgttcta tttttgtcgt tgtggcatta
     6121 atcccgtgga atatgccagg gctgaaagcc gttggttctt atcgctcggt tctggaattg
     6181 ctcaatattc ctcatgcgaa attaatcatg gactgcgtga tattactttc cgtaaccagc
     6241 tgcctgaact cggcgctgta taccgcttcc aggatgctct actccttaag ccgtcgcggt
     6301 gatgctcccg cggtaatggg taaaatcaac cgcagtaaaa ccccgtacgt ggcggtgtta
     6361 ctctccaccg gcgcggcatt cttaacggtg gtggtgaact attacgcgcc tgcgaaggtg
     6421 tttaaatttc tgatcgacag ctccggcgct atcgccctgc tggtttattt agtcatcgcc
     6481 gtttcacaat tgcggatgcg caaaattctg cgagcagaag gaagcgaaat tcgcctgcgg
     6541 atgtggcttt atccgtggct cacctggctg gtaatcggtt ttattacctt tgtgttggta
     6601 gtgatgctat tccgcccggc gcaacagtta gaagtgctct ccaccggctt attagcgatt
     6661 gggattatct gtaccgtgcc aattatggct cgctggaaaa agctgataat gtggcaaaaa
     6721 acacccattc ataatacgcg ctgataatga tcaggagtca caccatgacc attacctctc
     6781 tggatggcta tcgctggctg aagaacgata ttattcgcgg caattttcag ccggatgaaa
     6841 aattacggat gagtttgctg acatcgcgtt atgcacttgg cgttgggccg ttgcgggaag
     6901 cgctttcgca actggtggca gaacgactgg tcacggtggt gaatcaaaaa gggtatcggg
     6961 tggcgtctat gtcagagcag gagctgctcg atattttcga tgcccgtgcc aatatggaag
     7021 cgatgctggt aagcctggcg attgcccgcg gtagtgatga gtgggaggca gacgttcttg
     7081 cgaaggcgca tctgctcagt aagcttgagg cctgtgacgc cagtgagaaa atgcttgatg
     7141 agtgggatct gcgtcatcag gcgtttcata ctgcaattgt gacaggctgt ggttctcact
     7201 atttgctgca aatgcgtgaa cggttgtttg atctggcggc gcgctatcgc ttcatctggc
     7261 tgcggcgaac ggtactttcc gtggaaatgc tggaagataa acacaaccaa catcaaattt
     7321 taaccgaagc tgtgctggcg cgggatgccg cgcgcgccag tgagttaatg cgccagcatt
     7381 tgctgacgcc gattcccatt atccagcagg cgatggctgg caattaacta ctcttcagga
     7441 atacgcaaca cttgccccgg gtaaatttta tccgggcttt ttagcatcgg tttattcgct
     7501 tcgaagattt tattgtacag attagcgtta ccgtagacct gtttggaaat ggcactcaga
     7561 gtgtcgccag acttaacggt ataaaactgg ctggcagtgg ctggtatcgc cgttttcacc
     7621 tgatcatcga cactggcaat accggaaata ttccccaccg caacaaggat tttctccttc
     7681 gcctcctgac ttaagccgtc accagtgacc gtcgctttgc cgtcggcaat ttgaatgttc
     7741 actttatcgg catcaggtat accggttttg ttcagatgct cctgcacctt cttcgcctga
     7801 tcgtctttat cgtgctgacc tgtaaccgcg tcccagagtt tttctccggc atctttcaca
     7861 aaattgaaca gacccatagc tacctcattc gttaacggaa acactcagaa agtgtagcag
     7921 aggaacgcgg cgcgttaacg ccgcgggggt gagcacaggt ttaatcgcgg gtttgcaccc
     7981 agaacgcgtg aatcaaaccg gggatatagc caagcagtgt cagcagaata ttaataatga
     8041 acgcccaacc gaacccttta ccgagcagca cgccgagcgg cggcagaata atggtgatga
     8101 cgattctcca gaaacccata tgtactccct ataagaaaat tactcattgt ttaaaaagag
     8161 attttatctc ttaagcgtag taattttagc ggagcctgcc agtttcagct tacgctttta
     8221 ctctcattta cgcggctgag gctggtatat gttgcatttt tagtttagag tttacttaat
     8281 ttagaaaata cttaaatatc tatgactgaa ctcgcacaat tacaggccag tgccgaacag
     8341 gcagcggcct tattgaaagc aatgagccac cctaaacggt tgctgattct gtgcatgctt
     8401 agcggttccc ccggcaccag tgcgggagag ctgacgcgca ttaccggact gagtgcctct
     8461 gcgacatcac agcatctcgc tcgtatgcgg ggcgaagggc ttatcgacag ccaacgggat
     8521 gcccaacgca ttctatattc cattaaaaat gaggcggtaa atgccattat cgccaccctg
     8581 aaaaatgtct attgtccgta aggagtcact atggctttga caaccatttc gccgcatgat
     8641 gcacaagaat taatcgcacg cggcgcaaag ttaatcgata ttcgtgatgc tgatgaatat
     8701 cttcgtgaac atattcctga agcagatcta gctccattat ccgtactgga acagtcaggt
     8761 cttccgccta aattacgtcg cgagcaaatt atattccact gccaggcagg taaacgcacc
     8821 agtaataacg ccgataaatt agcagcgatt gccgcccccg cagaaatctt tttactcgaa
     8881 gatgggattg atggctggaa aaaagcagga ttgccagtag cggtaaataa atcccaaccc
     8941 ttgccgttaa tgcgccaggt gcagatcgct gcgggtggtt taatattaat cggcgttata
     9001 ctaggttata ccgtaaatag cggtttcttc ttattaagcg gctttgttgg tgccgggtta
     9061 ctgtttgcag gaattagcgg tttttgtggg atggcaaggt tgttagataa gatgccgtgg
     9121 aaccaacgag cttgagaagc gacgccggac gcgccatagc agcaacatcc ggcctcagta
     9181 attagatcag gaaatcgtcg agagatttac cttctgccag cgcctgagcg attggcttcg
     9241 gtgtacggcc ctgaccggtc caggttttag tttcaccgtt aacatcgatg aatttatatt
     9301 tcgccggacg cggctggcgt tttttaccag cgcgtggtgc cgcagcagag ctattaccca
     9361 ataactcttc cgggttaatt ccgtcagctt tcatcagctc cagccaggtg ctaatttttt
     9421 cctggcgctc tgccagttca cgctgctgtt gttcttcttc ttcacgtctt tctttagtga
     9481 caaccctgaa tttttcgagc atttcttcaa gaacgtcaat ggagaattcg cgagccatcg
     9541 cacggagggt gcgaatgtta tttaaacttt gtaacattac ggacataata aagaaaacct
     9601 tttaacccaa aa
//
LOCUS       DAZKHK010000164         9406 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKHK010000164 DAZKHK010000000
VERSION     DAZKHK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.165"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..272)
                     /locus_tag="ACHOS5_004436"
     CDS             complement(<1..272)
                     /locus_tag="ACHOS5_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP9779152.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNTHYSLQETHATPDRHPVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLH"
     gene            complement(468..755)
                     /locus_tag="ACHOS5_004437"
     CDS             complement(468..755)
                     /locus_tag="ACHOS5_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP9779153.1"
                     /translation="MRESELEGFIQVAPYPLEAVPYQLFAKMIGRKESTVRTMIDAAK
                     LPTIDFVKPGSVKTRASENWVYLPAFNAGMRKAFFNQPKEQRDAWLLWLGL"
     gene            860..1189
                     /locus_tag="ACHOS5_004438"
     CDS             860..1189
                     /locus_tag="ACHOS5_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779154.1"
                     /translation="MPDMASLSSMGEKFRLIREAEGMTRQEFADCVGLPYGTVTNYEV
                     RGKQVTEGALLKVTKHPKFKKYAYWLSTDETMPEAGQIAPALSLDGSCNLEGNQDSTE
                     TTRKLPR"
     gene            1286..2290
                     /locus_tag="ACHOS5_004439"
     CDS             1286..2290
                     /locus_tag="ACHOS5_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9779155.1"
                     /translation="MSIKKLEDGRYLLDIRPNGRKGKRVRKVFNKKSVAVATERYIMA
                     NAEKREYIQGYRDRRTLNDLLELWWVYHGQHRRKAEEDRKQLRNIINELGADMQAVDL
                     DKLKIIAWRSRRIADGLKPSSANRYMNRLSGMFTALKKIGLWDAEHPVRGISVLYVSP
                     REMAFLSQKEVALLLDTLEGDYWRVALLCLSTGARWGEASKLRGEQIVHNRVTFLETK
                     NGRKRTVPISQAVCEAIKTSETGGLFDVKYREFCLVLKRVKPDLPKGQAAHVLRHTFA
                     SHFVMNGGNIIALQRILGHATIQQTMAYAHFAPDYLQDAVALNPLKGGVSVHSVSTGE
                     "
     gene            2493..3605
                     /gene="pdxB"
                     /locus_tag="ACHOS5_004440"
     CDS             2493..3605
                     /gene="pdxB"
                     /locus_tag="ACHOS5_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP9779156.1"
                     /translation="MPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVRSVTKVNES
                     LLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERD
                     GFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRSLDELVQHA
                     DILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALLTCLSEGQK
                     LSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSKFIGHEQHV
                     ALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPGEFDKLRKN
                     YLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            3671..4684
                     /gene="usg"
                     /locus_tag="ACHOS5_004441"
     CDS             3671..4684
                     /gene="usg"
                     /locus_tag="ACHOS5_004441"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9779157.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDVFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            4684..5496
                     /gene="truA"
                     /locus_tag="ACHOS5_004442"
     CDS             4684..5496
                     /gene="truA"
                     /locus_tag="ACHOS5_004442"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9779158.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            5579..6238
                     /gene="dedA"
                     /locus_tag="ACHOS5_004443"
     CDS             5579..6238
                     /gene="dedA"
                     /locus_tag="ACHOS5_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9779159.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            6394..7308
                     /gene="accD"
                     /locus_tag="ACHOS5_004444"
     CDS             6394..7308
                     /gene="accD"
                     /locus_tag="ACHOS5_004444"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9779160.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            7378..8646
                     /gene="folC"
                     /locus_tag="ACHOS5_004445"
     CDS             7378..8646
                     /gene="folC"
                     /locus_tag="ACHOS5_004445"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP9779161.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            8636..9298
                     /gene="dedD"
                     /locus_tag="ACHOS5_004446"
     CDS             8636..9298
                     /gene="dedD"
                     /locus_tag="ACHOS5_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP9779162.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
BASE COUNT         2158 a         2198 c         2750 g         2300 t
ORIGIN      
        1 tcgtgcaggg tgaaatcctg cccgaactgc tcatctccgc gagtcaggga gtaacaggta
       61 tttccggtaa tcgggttgcg tgcgcctctg tgtatgacaa ttccggcatc attaatcagc
      121 caggtgtgtt taccggtttg tttaaccgga tgcctgtcag gtgtggcgtg cgtttcctgc
      181 aggctgtagt gcgtgttgct gcgtgatgca ctggttgaga aacgggctgc gtgacgttct
      241 gtgccagtgc ggaagctgcg tcgctgtgtc acatcggaaa agacaacgcc agccacacca
      301 ctacgtacgt tgtatcgttt aattatggtt tttacttttt gtgtagccat ggctgtttcc
      361 ttttgtgtct gtagtgggtt atcagcttag ttatcctgtg cttttttatg ccgaaacaac
      421 tctctcagag tagatatatt ggttttgaaa aattcctgga tatatcgtta cagacccagc
      481 cataacagcc atgcatcgcg ttgttctttg ggttgattaa aaaatgcttt tcgcatacct
      541 gcgttgaatg ctggcagata tacccagttt tctgatgcgc gggttttcac tgaacccggt
      601 ttcacaaagt caattgttgg tagctttgcg gcgtcaatca tggttctgac ggtggattct
      661 ttgcggccaa tcatcttggc gaatagttga tatggcaccg cctcaagtgg gtatggcgct
      721 acctgaatga agccctcaag ctctgattct ctcattgtgg taatctcctt gattcgttcg
      781 aatggctcaa aatggcccaa agtggcttat tttggcttct tatgtgtgtg cccgatgtgg
      841 cacgtttgca atggaggata tgcccgatat ggcatctttg tcaagcatgg gtgaaaaatt
      901 tcgcctgata cgtgaagctg aaggtatgac ccgccaggag tttgcggatt gcgtggggtt
      961 gccttatggg acagtaacta actatgaggt gagggggaag caagtaacag agggggcctt
     1021 acttaaggtt acaaaacacc ctaaatttaa gaagtacgcc tactggttat caactgatga
     1081 aacaatgccg gaggccggac agatagcccc ggctctctct cttgatggct cctgcaattt
     1141 ggagggcaat caggactcaa ccgaaacaac ccgaaaatta ccccgctgag gccagaaaac
     1201 tggttggacc tgctttttgc ctggtctgac tattgctggg aagaagctgg tgaaattgta
     1261 aaacggttca ttggagggct tcgccatgtc gattaagaag ctcgaagatg gtcgttattt
     1321 gctggatatc aggccgaacg gacgcaaggg aaaacgcgtg cgtaaggtat ttaacaaaaa
     1381 atcggtagcg gtggccactg agcgctacat catggcgaat gctgagaagc gggaatatat
     1441 acagggctac cgtgatcgcc ggacgttgaa cgatttgctg gagttgtggt gggtgtatca
     1501 cggccagcac aggcgtaaag cggaggaaga ccgaaaacaa ttacgcaaca taatcaatga
     1561 acttggtgct gatatgcagg cggtggatct ggataagctg aaaatcattg cctggcgttc
     1621 tcgcaggatc gctgacggac tgaagccgtc atcggctaac aggtatatga accggttatc
     1681 cggaatgttt accgctctga aaaaaatagg actatgggat gcagagcacc cggtcagggg
     1741 aatctctgtg ctttatgtgt ccccgcgaga aatggctttc ttgtcccaga aagaagtggc
     1801 gttattgctc gatacactag agggcgatta ctggcgtgtt gcgcttttgt gtttaagtac
     1861 gggggcacgc tggggcgaag ccagtaagct tcgtggtgaa cagatagttc ataatcgtgt
     1921 gacgtttctt gagaccaaaa acggtcgtaa gagaacggtg ccaatctcgc aggcggtttg
     1981 tgaggcgatc aaaaccagcg agacgggggg attatttgat gtgaagtacc gggaattttg
     2041 cctggtgctg aaaagggtta aacccgattt gccaaaagga caggcggcgc atgtgttgcg
     2101 acatacgttc gccagtcatt ttgtgatgaa cgggggaaac attattgcgc tacagaggat
     2161 cctgggtcac gcgactatcc agcaaacaat ggcgtatgca cattttgcgc cggattattt
     2221 gcaggatgcg gtggctctta atccgctgaa aggtggcgta agtgtccact cggtgtccac
     2281 gggagagtga ttttgtctgg tctgaaatag ctttaagggt gatactgaga aacatacttt
     2341 ccagtatcac ccgtctaaat ggttgcgttt gtatgtgtat gattatgaaa tggttttcat
     2401 gttgtacggt ggggttaaaa gggtgtttgc tgataaaggg ggccagattg tcgcgtactg
     2461 aaaaattatt aacgatcctt gttgatgaaa atatgcctta tgcccgcgac ttatttagcc
     2521 gtttgggtga ggtgaccgcg gttcccgggc gtccaattcc tgtcgctcaa ctggcagacg
     2581 cggatgcgct tatggtgcgt tcggtcacga aagtgaatga atctttgctg gcaggaaaac
     2641 ccattaaatt tgttggcact gccaccgcgg ggaccgacca tgtcgatgaa gcatggttaa
     2701 agcaggcggg aattggtttt tccgctgcac ctggctgtaa tgcgattgcg gtagtggaat
     2761 atgttttctc ctccctgctg atgcttgccg aacgcgatgg attttcactg cacgagcgta
     2821 ccgtggggat cgttggcgtt ggtaacgttg gtcgtcgttt acaggcgcga ctggaagctt
     2881 tagggatcaa aaccttactt tgcgatccgc cccgcgccga ccgtggggat gagggggatt
     2941 tccgctcgct ggatgaatta gttcagcatg cggatattct gactttccat acgccactct
     3001 ttaaagatgg tccgtacaaa acgctgcatc tggcggatga aaaactgatc cgcagcctga
     3061 aaccgggagc gatcctgatt aacgcctgcc gtggcgcagt cgtcgataat accgccctgc
     3121 tgacctgcct gagcgaaggc cagaagttaa gcgtagtgct ggatgtctgg gaaggcgaac
     3181 cggaacttaa cgtggaattg ctgaaaaaag tggatatcgg cacgccgcat atcgcaggct
     3241 ataccctgga aggtaaagca cgcggtacta cgcaagtgtt tgaagcttat agcaagttta
     3301 ttgggcatga acagcacgtt gcgctggata cattactgcc cgcgccagag tttggtcgca
     3361 ttacgctgca tggcccgctc gatcaaccga cgctgaaaag gctggtgcat ttggtgtatg
     3421 atgtgcgccg cgatgacgca ccgctgcgta aagtcgccgg gataccgggt gagttcgata
     3481 aactgcgcaa aaactatctt gagcgccgtg aatggtcatc tctgtatgtg atttgtgatg
     3541 acgccagtgc ggcatcattg ctgtgtaaac tgggttttaa cgccgttcat catccggcac
     3601 gttaatctct tcttcatgct ctctgctgta acattggcag ggagctttgc tatttctgga
     3661 gtaaaccacc atgtctgaag gctggaacat tgccgtcctg ggcgcaactg gcgctgtggg
     3721 cgaagccctg cttgaaacgc tggctgaacg tcagttcccg gttggggaaa tttatgcact
     3781 ggcacgtaac gaaagcgcag gagaacaact gcgctttggt ggtaagacaa tcaccgtgca
     3841 ggatgccgct gaattcgact ggacgcaggc gcagctggcg ttttttgtcg caggcaaaga
     3901 agctaccgct gcctgggttg aagaagcgac caactcaggt tgtctggtga tcgacagcag
     3961 cggattgttt gctctcgaac ctgacgtacc gctggtggtg ccggaagtaa acccgtttgt
     4021 actgaccgat taccgcaacc ggaatgtcat cgccgtacca gacagtctga ccagccagct
     4081 gctggcggca ctgaaaccgc taattgatca gggcggttta tcacgtatca gcgttaccag
     4141 cctgatttca gcctccgccc agggcaaaaa agcggtcgat gcgttagcgg ggcagagtgc
     4201 taaattactc aatggcattc cgattgacga agaagatttc ttcgggcgtc agctggcgtt
     4261 caacatgctg ccgttactgc cggatagcga aggtagcgtg cgtgaagaac gtcgtatcgt
     4321 tgacgaagta cgcaaaatcc tgcaggacga agggctgatg atttcggcaa gcgtcgtcca
     4381 ggcaccggta ttctacggtc atgcccagat ggtcaacttt gaagcactgc gtccgctggc
     4441 ggcagaagaa gcgcgtgatg tgtttgctca gggcgaagat attgtgctct ctgaagagaa
     4501 cgaattcccg actcaggtag gagatgcttc tggtacgccg catctttctg ttggctgcgt
     4561 acgtaatgac tacggtatgc cggagcaagt tcagttctgg tcggtggccg ataacgttcg
     4621 ctttggcggc gcgctgatgg cagtaaaaat cgccgagaaa ctggtgcagg agtatctgta
     4681 ctaatgtccg accagcaaca accgccagtt tataaaattg cgctgggcat tgagtacgac
     4741 ggcagtaggt attacggctg gcaacggcag aatgaagtcc gcagtgtgca ggagaagctg
     4801 gaaaaggcgc tctcccaggt ggcgaacgaa cccatcaccg tcttctgcgc cgggcgtact
     4861 gacgcagggg tacacggtac cgggcaggtg gtgcatttcg aaacgaccgc gcagcgcaaa
     4921 gacgctgcgt ggacgttagg cgtaaatgcg aatttacctg gtgacatcgc cgtgcgttgg
     4981 gttaaagctg tacctgatga ttttcatgcc cgatttagcg ccacggctcg ccgttatcgc
     5041 tacatcatct acaatcatcg gctgcgcccg gcggtactga gtaaaggagt aacccatttt
     5101 tacgagccgc tggacgctga acggatgcat cgggctgcgc aatgcttgct gggcgagaat
     5161 gatttcacct cgttccgtgc ggtgcagtgt cagtcccgaa ccccgtggcg caacgttatg
     5221 catattaatg tcacgcgtca cggtccttat gtggtggtag atatcaaagc gaatgccttt
     5281 gtacatcata tggtcaggaa tattgtcggc agcctgatgg aagtcggtgc ccacaaccag
     5341 ccggagagct ggatagcaga gttgctggcg gcaaaggaca gaacgctggc ggcagcaacg
     5401 gcaaaagcgg aagggctata tctggtcgcg gtggattacc ctgaccgata tgatcttcca
     5461 aaaccgccaa tgggcccgct atttctggcg gactaacgag aataatgctc ggaacatttc
     5521 aggctgtagc caacgtagtt acagcctgat agatgacgag tacaaggcat aggcaaatat
     5581 ggacctgatt tatttcctca tcgattttat cctgcacatt gacgtgcatc tggcagagct
     5641 ggtcgcggag tacggcgtct gggtttatgc cattttgttc ttaattttgt tctgtgaaac
     5701 cggtctggtg gtaacgccgt ttttaccggg tgattcattg ctgtttgttg ccggagcgtt
     5761 ggcatcactg gagaccaacg atctcaacgt ccatatgatg gtggtgctga tgctgatcgc
     5821 cgcgattgtg ggtgacgcgg tcaattacac tatcgggcgg ttgttcggtg aaaagttatt
     5881 cagtaatccc aattcgaaaa ttttccgtcg cagttatctc gacaaaaccc atcagtttta
     5941 tgagaaacat ggcggcaaaa cgattattct cgcccgtttt gtgccgatcg tcagaacgtt
     6001 cgccccgttt gttgcgggaa tgggccacat gtcgtaccgt catttcgccg cttataacgt
     6061 gattggcgca ctgttgtggg tactgctttt tacctacgca ggctatttct tcggcacgct
     6121 ccccttcatt cagagcaacc ttaaactgat gattgttggc attatttttg tttcaatatt
     6181 gccaggtgtt ttcgaattta ttcgccacaa acgcgcagcg gcacgcgccg caaaataaaa
     6241 agtaactctg cggttcgacc acttttttat ccaaagtttc gggctgttat gttttaatgt
     6301 gcaacattca tggtctgttg ggggcaaaaa tggcattatg cgtccccaaa gataaaactg
     6361 gcatcgaacc aggttcagac agaaaggtcc ctaatgagct ggattgaacg aattaaaagc
     6421 aacattactc ccacccgcaa ggcgagcatt cctgaagggg tgtggactaa gtgtgatagc
     6481 tgcggtcagg ttttataccg cgctgagctg gaacgtaatc ttgaggtctg tccgaagtgt
     6541 gaccatcaca tgcgtatgac agcgcgtaat cgcctgcata gcctgttaga tgaaggaagc
     6601 cttgtggagc tgggtagcga gcttgagccg aaagatgtgc tgaagtttcg tgactccaag
     6661 aagtataaag accgtctggc atctgcgcag aaagaaaccg gcgaaaaaga tgcgctggtg
     6721 gtgatgaaag gcactctgta tggaatgccg gttgtcgctg cggcattcga gttcgccttt
     6781 atgggcggtt ccatgggatc tgtggtaggt gcgcgtttcg tgcgtgccgt tgagcaggcg
     6841 ctggaagata actgcccact gatctgcttc tccgcctctg gtggcgcacg tatgcaggaa
     6901 gcactgatgt cgctgatgca gatggcgaaa acctctgcgg cactggcaaa aatgcaggag
     6961 cgcggcttgc cgtacatctc cgtgctgacc gacccgacca tgggcggtgt ttctgcaagt
     7021 ttcgccatgc tgggcgatct caacatcgct gaaccgaaag cgttaattgg ctttgccggt
     7081 ccgcgagtta tcgaacagac cgttcgcgaa aaactgccgc caggattcca gcgcagtgaa
     7141 ttcctgatcg agaagggcgc gatcgacatg atcgtccgtc gtccggaaat gcgcctgaaa
     7201 ctggcgagca ttctggcgaa gttgatgaat ctgccagcgc cgaatcctga agcgccgcgt
     7261 gaaggcgtag tggtgccacc ggtaccggat caggaacctg aggcctgata actgataagg
     7321 gcagggccac tggctctgcc cttttgctat tctcaccgta acgaatcagc ggataccatg
     7381 attatcaaac gcactcctca agccgcgtcg cctctggctt cgtggctttc ttatctggaa
     7441 aacctgcaca gtaaaactat cgatctcggc cttgagcgcg tgagccaggt cgcggcgcgt
     7501 cttggcgttc tgaaaccagc gccatttgtg tttaccgttg cgggtactaa tggcaaaggc
     7561 accacctgcc gtacgctgga gtcgattctg atggcggcag ggtacaaagt gggcgtctac
     7621 agttcgccgc atctggtgcg ttataccgag cgcgtacgtg tgcagggtca ggaattgccg
     7681 gaatcggccc acaccgcttc ttttgcggag attgaatcgg cacgcggtga tatttccctg
     7741 acctatttcg agtacggtac gctctcggcg ttgtggctgt tcaagcaggc acaacttgac
     7801 gtagtgattc tggaagtagg gctgggcggt cgtctggacg caaccaatat tgtcgatgcc
     7861 gatgtcgcgg tagtaaccag tattgcgctg gatcataccg actggctggg gccagatcgt
     7921 gaaagtattg gtcgcgagaa agcaggtatc tttcgcagcg aaaaaccggc aattgtcggt
     7981 gagccggaga tgccttctac cattgctgat gtggcgcagg aaaaaggtgc actgctacaa
     8041 cgtcggggcg ttgagtggaa ctattccgtc accgatcatg actggacgtt tagcgatgct
     8101 cacggcacgc tggaaaatct gccgttgccg cttgtcccgc aaccgaatgc cgcaacggcg
     8161 ctggcggcac tgcgtgccag cgggctggag gtcagtgaaa atgccattcg cgacgggatt
     8221 gccagcgcaa ttttgccggg acgtttccag attgtgagcg agtcgccacg cgttattttt
     8281 gatgtcgcgc ataatccaca tgcggcggaa tatctcaccg ggcgtatgaa agcgctaccg
     8341 aaaaacgggc gcgtgctggc ggttatcggt atgctacatg ataaagatat tgccggaact
     8401 ctggcctggt tgaaaagcgt ggttgatgac tggtattgtg cgccactgga agggccgcgc
     8461 ggtgccacgg cagaacaact gcttgagcat ttgggtaacg gcaaatcatt tgatagcgtt
     8521 gcgcaggcat gggatgccgc aatggcggac gctaaagcgg aagacaccgt gctggtgtgt
     8581 ggttcgttcc acacggtcgc acatgtcatg gaagtgattg acgcgaggag aagcggtggc
     8641 aagtaagttt cagaatcggt tagtgggtac gatcgtgctg gtggcgctgg gggtgattgt
     8701 acttccaggg ctgctggacg ggcagaaaaa gcattatcag gatgagttcg ctgctatccc
     8761 gctggtgccg aaagcgggcg atcgcgatga gcctgatatg atgccagccg ccacccaggc
     8821 gttgccgacg cagccgccgg aaggcgcagc ggaagaggta cgggcaggtg atgccgcagc
     8881 accgtcgctc gatccggcca ctattgcagc caataacacc gagtttgaac cggaacctgc
     8941 accggtcgcc ccaccgaagc cgaaaccggt ggagccgctt aagccaaagg ttgaagcacc
     9001 acctgcgccg aagccagaac cgaagccggt cgtggaagaa aaagcagcgc caacgggtaa
     9061 agcttatgtt gtgcaactgg gcgcgctgaa aaatgccgat aaagtgaatg agattgtcgg
     9121 taagctgcgc ggtgccggtt atcgggttta tacgtcgcca tccacgccag tgcagggtaa
     9181 aattacccgt attctggttg ggccggatgc ttccaaagag aagctgaaag gatcgctggg
     9241 tgagctcaag cagctttctg gcttaagtgg cgtggtaatg ggctatacac cgaattaata
     9301 cggtcttgcc tgatgcgacg ctggcgcatc ttatcaggcc tgtgcagggg tagaacagta
     9361 ggtcggataa ggcgttcacg ctgcatccga cagccgttgc ctgatg
//
LOCUS       DAZKHK010000165         9328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKHK010000165 DAZKHK010000000
VERSION     DAZKHK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.166"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(57..806)
                     /gene="yafL"
                     /locus_tag="ACHOS5_004447"
     CDS             complement(57..806)
                     /gene="yafL"
                     /locus_tag="ACHOS5_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9779163.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKVSDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLGKPYIWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1118..1858
                     /gene="dpaA"
                     /locus_tag="ACHOS5_004448"
     CDS             1118..1858
                     /gene="dpaA"
                     /locus_tag="ACHOS5_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP9779164.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1829..2596)
                     /gene="yafJ"
                     /locus_tag="ACHOS5_004449"
     CDS             complement(1829..2596)
                     /gene="yafJ"
                     /locus_tag="ACHOS5_004449"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP9779165.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMATVFKYIA
                     SLVDELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCFGERVV"
     gene            complement(2801..3379)
                     /gene="lpcA"
                     /locus_tag="ACHOS5_004450"
     CDS             complement(2801..3379)
                     /gene="lpcA"
                     /locus_tag="ACHOS5_004450"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP9779166.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3619..6063
                     /gene="fadE"
                     /locus_tag="ACHOS5_004451"
     CDS             3619..6063
                     /gene="fadE"
                     /locus_tag="ACHOS5_004451"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP9779167.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILIPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTTEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKVLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6106..6552)
                     /gene="ivy"
                     /locus_tag="ACHOS5_004452"
     CDS             complement(6106..6552)
                     /gene="ivy"
                     /locus_tag="ACHOS5_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP9779168.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6733..7503
                     /gene="yafV"
                     /locus_tag="ACHOS5_004453"
     CDS             6733..7503
                     /gene="yafV"
                     /locus_tag="ACHOS5_004453"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP9779169.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQYNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQTYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYREKFPAWRDADEFRLL"
     gene            complement(7555..7749)
                     /locus_tag="ACHOS5_004454"
                     /pseudo
     CDS             complement(7555..7749)
                     /locus_tag="ACHOS5_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(7775..7975)
                     /locus_tag="ACHOS5_004455"
     CDS             complement(7775..7975)
                     /locus_tag="ACHOS5_004455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4695 family integrating conjugative element
                     protein"
                     /protein_id="HFP9779170.1"
                     /translation="MSEHLEILKSLNTLGLVVNVESVERMEVLQKRADGLLLLPVICD
                     NFVQMLQLNAYPVLITEMEISQ"
     gene            8365..9174
                     /locus_tag="ACHOS5_004456"
                     /pseudo
     CDS             8365..9174
                     /locus_tag="ACHOS5_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011146066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(9191..9313)
                     /locus_tag="ACHOS5_004457"
     CDS             complement(9191..9313)
                     /locus_tag="ACHOS5_004457"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9779171.1"
                     /translation="MANNLGVSIKTVSSYKKSLMNRLYVSNDIQLFYKGLLLCR"
BASE COUNT         2347 a         2288 c         2349 g         2344 t
ORIGIN      
        1 ggttaactga aactgtccag gttttggggt tcagttcagt gaacgcctta tccggcctac
       61 aaaatcgtct cttccgtcaa aatccgccgc gcgcctaaaa agtgatcctg ccagaaaggt
      121 tcagctaacc gacttacccg aatggtttcc ccggtgcgcg gtgactcgat aaactgaccg
      181 tctccaagat atacgcccat atggtcggct atctcacgac tgtggatatg gaaaaacagc
      241 aaatctcccc ggcgcaggtc gttattcgcc acaatcgttg cccgacggta gtggtacatc
      301 tcattggccg tgcgcgggag cttagcctca aggatcttgt tgtaggcata aaaaaccaac
      361 ccgctacagt cgaagccttt atcaggccgc gtgccccccc agatataagg cttacctagc
      421 tgctgctcca ggcggtgaat cgccacttcg gtgatgttat gtagatggtc gctgcttaag
      481 aaatgattat tctccaccaa cgcctgccag cgtctgtcac tcaccttgag cggcgcagga
      541 aaccattctg gatgctgttt tatctgctcc cgattatgct ggcgcagcgc ccttctgctt
      601 ttggcattgc cttccacaat atagctggcc tgctttttca gatgggtttg gtactgtttt
      661 aataaacgcg cacggttttg catccgctgg cgggcggcgt agctaaaaga gatatgtgat
      721 gtggtggcgc tggcgagtaa agggagcaaa acgcataacg aaaacacgcc tggatgcaga
      781 aatctgccgt gcaagaacga agacatgaag gacataaacc agctgaatga aggtgagaat
      841 tttaaaagac cggaggagta aacgggatta ttaattgctt atttgcatat tactattgcg
      901 gtttcataat cttaggaaat aagaaataaa tctacaatcc atgcgctttt cggactcttg
      961 aaaactgccc ctgtgctaaa tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt
     1021 tggtgataat gtgagccttc ccaatccaga ctattccgat ttgggatcca ttttgccgtc
     1081 ctggcgtgtg taaccgtttt atcaaggaat aagcagtatg cgtaaaatcg cattaattct
     1141 tgcgatgttg ttgatcccgt gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac
     1201 cacgccagtg agcaaagagt ataagcagca gttgatggga tcccctgtct acatccagat
     1261 cttcaaggaa gaacgtacgc tcgatctcta cgtcaaaatg ggcgagcaat atcaactgct
     1321 cgacagctat aaaatctgta aatattccgg cggcttaggg ccaaaacagc gtcagggcga
     1381 tttcaaaagc ccggaagggt tttatagcgt ccagcgtaat cagttaaaac cagacagccg
     1441 ttactacaaa gcgattaata ttggattccc aaatgcctat gaccgtgcgc atggttatga
     1501 agggaaatac ctgatgattc acggcgattg tgtttccatc ggctgctacg cgatgaccaa
     1561 tcagggtatt gatgagatct tccagttcgt tactggtgcg ctggtgtttg gtcagccgag
     1621 cgtgcaagtg agtatttatc cgttccgcat gaccgacgcc aatatgaagc gccataaata
     1681 ttccaacttt aaggacttct gggagcaact gaagccgggc tacgactact ttgagcaaac
     1741 ccgtaagcca ccgaccgttt cagtggtcaa tggccgttac gtggtcagca agccgttaag
     1801 ccacgaagtt gtgcagccac aactggcatc aaactacacg ctccccgaag caaaataagc
     1861 gccattcacc tggcataatc ttttgccagg tttcattgcc cgtcagcggc tgtgtcgcaa
     1921 tcaccgtgac cacatcattc ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat
     1981 ccagcaacgt tgccacgcca aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc
     2041 aatacgccat tacatagcgc ccatccgaaa gtagcatgtt gaaaacgccc ttctgccgca
     2101 gttcatccac cagtgaggcg atatatttaa acactgtcgc catgttgccc ggtgtgcgcg
     2161 ggtaacgctg cgttaattta tgcaggagcc agcaaaaggc tttttcgcta tcggtttcac
     2221 cgaccgggcg gaagttgccg gtttccagtg atttgtagcc cgtcagttgt ccattatggg
     2281 cataagtcca gttacgcccc cataactcgc gggtaaacgg gtgagtattt tccagcgcca
     2341 cctcgccccg attcgcctgg cgaatatgag ccaccaccga acaggatttt atcgggtagt
     2401 cctggacaag tttggcgatg ggggaattaa agctaggttg cggatcttta aatgtgcgac
     2461 agcctttacc ttcgtaaaag gtaatgcccc agccatcttt atgtggcccg gttccaccac
     2521 cacgctgtac aagtccggtg aaactaaagc agatatcggt agggacgttg gcactcatcc
     2581 cgagcaattc gcacatgggt aacctccaca acagcgggag cgcatggccc ccggcgaaga
     2641 ttgatttaag aattttcagg tcggatgtgg cgtgaacgca ttattcggcc tacggttcgg
     2701 gtgcagattg taggcatgat aagacgcgtc agcgtcgcat caggcatcag agcacaaatg
     2761 ccggatgcgg cgtaaacgcc ttatctggcc tacgccagac ttacttaacc atctcttttt
     2821 caattaactg gatcaggata tggatcactt taatgtggat ctcctgaatg cggtcggcat
     2881 aaccaaagtg cggaacgcga atttcaatat ccgccgtgcc agccattttg ccgccgtctt
     2941 tacccgtcag ggtgatcact ttcatcccct tctcacgcgc cgctgcgatc gctttgatca
     3001 cgtttgcaga gttaccggag gtggagatcc ccagcagtac atcgccttcg cgacctaccg
     3061 cttcaacgta acgggagaaa atatcattga aaccaaaatc gttaccgacg caggaaatat
     3121 gactaacgtc agaaatggca atcgccgggt agcccggacg gttttcacgg tagcgaccgg
     3181 tcaactcttc ggcaaagtgc atggcgtcgc aatgggagcc gccgttgcca caggaaagca
     3241 ctttgccacc ggctttaaag ctgtctgcta acaggaccgc cgcgcgctga atggcgtgaa
     3301 tattggcgtc atcttttaaa aagttagcca gcgtttccgc cgcttcgttc agttcattac
     3361 gaataagatc ctggtacatg aggatatcct tcagcataaa tgtaatagac aaaatgcagt
     3421 gtaccggata ccgccaaaag cgagaagtac gggcaggtgc tatgaccagg actttttgac
     3481 ctgaagtgcg gataaaaaca gcaacaatgt gagctttgtt gtaattgtat tgtaaacaca
     3541 ttgctaaatg tttttacatc cactacaacc atatcatcac aagtggtcag acctcctaca
     3601 agtaaggggc ttttcgttat gatgattttg agtattctcg ctacggttgt cctgctcggc
     3661 gcattgttct atcaccgcgt gagcttattt atcagcagtc tgattttgct cgcctggaca
     3721 gccgtcctcg gcgttgctgg tctgtggtcg gcgtgggtac tagtgcctct ggccattatc
     3781 ctcataccgt ttaactttgc ccctatgcgt aaatcgatga tttctgcgcc agtgtttcgt
     3841 ggattccgca aagtgatgcc gccgatgtcg cgcaccgaga aagaagcgat cgacgcgggc
     3901 accacctggt gggagggcga cttgttccag ggcaagccgg actggaaaaa gctgcataac
     3961 tatccgcagc cgcgcctgac cactgaagag caagcgtttc tcgacggtcc ggtagaagaa
     4021 gcctgccgga tggcgaatga tttccagatt actcatgagc tggcggacct gccgccggag
     4081 ttgtgggcgt accttaaaga gcatcgtttc ttcgcgatga tcatcaaaaa agagtacggc
     4141 gggctggagt tctcggctta tgcccagtct cgcgtgctgc aaaaactctc cggcgtgagc
     4201 gggatcctgg cgattaccgt cggcgtgccg aactcattag gcccgggcga gctgctgcaa
     4261 cattacggca ctgacgagca gaaaaatcac tatctgccgc gtctggcgcg tggtcaggag
     4321 atcccttgct ttgcactgac cagccccgaa gcgggttccg atgcgggcgc gattccggat
     4381 accgggattg tctgcatggg cgaatggcag ggccagcagg tgctggggat gcgtctgacc
     4441 tggaacaaac gctacattac gctggcaccg attgcgaccg tgcttggtct ggcgtttaaa
     4501 ctctccgacc cggagaaatt actcggtggt gcagaagatt taggtattac ctgtgcgctg
     4561 atcccgacca ccacgccggg tgtggaaatt ggtcgtcgcc acttcccgct gaacgtaccg
     4621 ttccagaacg gaccgacgcg cggtaaagat gtgttcgtgc cgatcgatta catcatcggc
     4681 gggccgaaaa tggccgggca aggctggcgg atgctggtgg agtgcctctc ggtaggccgc
     4741 ggcatcaccc tgccttccaa ctcaaccggc ggcgtgaaat cggtagcact ggcaacgggt
     4801 gcgtatgcgc acattcgccg tcagttcaaa atttctattg gtaagatgga agggattgaa
     4861 gagccgctgg cgcgtattgc cggtaatgcc tatgtaatgg atgctgcggc atcgctgatt
     4921 acctacggca ttatgctcgg tgaaaaaccc gccgtgctgt cggctatcgt taagtatcac
     4981 tgtacccacc gcgggcagca gtcgattatt gatgcgatgg atatcactgg tggcaaaggc
     5041 attatgctcg ggcaaagcaa cttcctggcc cgtgcttacc agggggcacc gattgccatc
     5101 accgttgaag gggcaaatat tctgacccgt agcatgatga ttttcggtca gggggcgatt
     5161 cgttgccatc cgtacgtgct ggaagagatg gaagcggcga agaacaatga cgtcaacgcg
     5221 ttcgataaac tcctgtttaa acatatcggt cacgtcggta gcaacaaagt tcgcagcttc
     5281 tggctgggcc tgacgcgcgg tttaaccagc agcacgccaa ccggcgatgc cactaaacgc
     5341 tactatcagc acctgaaccg cctgagcgcc aacctcgccc tgctttctga tgtctcgatg
     5401 gctgtgctgg gcggcagcct gaaacgtcgc gagcgtatct cggcacgtct gggggatatt
     5461 ttaagccagc tttacctcgc ctctgccgtt ctgaagcgtt atgacgacga aggccgtaat
     5521 gaagccgatc tgccgctggt gcactggggc gtacaggatg cgctgtatca ggcagaacag
     5581 gcgatggacg atttactgca aaacttcccg aaccgcgtgg ttgccgggct gctgaatgtg
     5641 gtgatcttcc cgaccggacg tcattatctg gcaccttctg acaagctgga tcataaggtg
     5701 gcgaagattt tacaagtgcc gaacgccacc cgttcccgca ttggtcgcgg tcagtacctg
     5761 acgccgagcg agcataaccc ggttggttta ctggaagagg cgctagtgga tgtgattgcc
     5821 gccgacccaa ttcatcagcg gatctgtaaa gagctgggta aaaacctgcc gtttacccgt
     5881 ctggatgaac tggcgcacaa cgcgctggcg aaggtgctga ttgataaaga tgaagccgct
     5941 attctggtga aagctgaaga aagccgtctg cgcagtatta acgttgatga ctttgatccg
     6001 gaagagctgg cgacgaagcc ggtaaagttg ccggagaaag tgcggaaagt tgaagccgcg
     6061 taattgaata acggagccga aaggctccgt ttctttatcc gctaattatt taaaattaaa
     6121 gccatccgga tggttttcca ggctgccggt caacgccgcg aacagcaccg ttttaccatc
     6181 aatagaaagc gcatcgttca cattcaacca ggtgagtttc tcttgcgacg ttttctcatc
     6241 aatggtcgag aacagccccg tcatctgatt agatttctcg gaccacatca cagcgatacg
     6301 ttgcgagcca cagtcatgcg gtttgcacgc gctcatcacc tgatacgtct catctcccaa
     6361 cgttacggtt tgtgcgggag tatacgtacc gcctttcatc acccaggcag gcagcttatg
     6421 cccttgcacc atctgattaa aagcagcttt ggtggtttcg ccctttgcaa ggctgctaat
     6481 ggttaaatca tcctgcgcca ttgcactggt ggcgatgacc agagcggcga ctgtcgttat
     6541 tgccttaaac atcattcctc ccgggcttat cctgcccatg ttattaacct cctaaagctt
     6601 ggcgataaca cgataatttg caaatcagat atttccaata tcagcatttg ttatcaattt
     6661 attctgcgct aaggcaactg cctgataagg tgttaaagtt ataaaaccct tcgcataaag
     6721 gagttgtaca ccgtgcctgg tttgaagatt acgcttttgc agcaaccact ggtgtggatg
     6781 gatggccctg ccaatctgcg ccattttgat cgtcaactgg aaggtattac cgggcgcgat
     6841 gtgatcgttc taccggaaat gtttaccagc ggctttgcca tggaagcggc agcttcgtcg
     6901 ctagcacaag atgacgtagt gaactggatg acagccaaag cgcagcagta caatgcgctg
     6961 attgcaggca gtgttgcatt acaaacggag tctggttcgg ttaaccgctt tttgctggtt
     7021 gagccgggcg gcacggtaca tttttatgat aagcgtcatc tgttccgcat ggcagatgag
     7081 catctacatt ataaagcggg caatgcgcga gtgattgtgg aatggcgcgg ctggcgtatt
     7141 ttgccgctgg tgtgctacga cttacgtttt ccagtgtggt cgcgcaatct caacgattat
     7201 gacctcgccc tgtacgtcgc caactggcct gctccgcgct ctctgcactg gcaggcattg
     7261 ctgacggccc gcgcgattga gaatcagacg tatgtagcgg gatgcaatcg cgtcggcagc
     7321 gatggcaacg gctgccatta tcgcggtgac agccgggtga ttaatccaca gggcgagatt
     7381 atcgctactg ccgacgcgca tcaggcaacg cgcattgatg cggagttgtc gatggctgta
     7441 ttaagggaat atagagaaaa gtttccggca tggcgggatg cggatgagtt taggttgctg
     7501 tgagtgggtg ttgcgtttag ggccggggag agtcggggta agactacgaa agcccgctct
     7561 cctgcgacgg cttttatcat aggagtgtgg aagtgctttt ccatcaatcg cgatgacgtc
     7621 tttgtcatct gaagaatggc attcacgcat ccggttaata aagcattcgt gaaattatac
     7681 tgaacggata caagacataa ctctaattat agtgccgtga acaggattac cactttcaaa
     7741 attacgatat tattagcagt aattatgtta ttaatcattg tgatatttcc atctctgtga
     7801 tcaataccgg ataagcattt aattgcagca tttgcacgaa attatcgcag ataactggca
     7861 ataacaataa cccatcagcc cgcttctgta atacctccat tcgttctact gattcaacat
     7921 taacaaccaa accaagggta tttagcgatt taaggatctc cagatgttca ctcatccagc
     7981 gcagagaaaa aggatcatca ccaattataa aaacaggccc ctagcaggtt aataactggc
     8041 gtttcattac ggatctagga tgaagtcgag tacatattac aggaaatacg ccctgttgcg
     8101 atggttccga tgatgtggaa tattcagaaa tatttccatt gttagtgaag aattaacatc
     8161 cattaataat ggtgaaacag cctcactttc cttgctagta atggctgcac taatctgaaa
     8221 ggaaaataac aatattatta cgtataaact catttttaac ataatacatt caatccgttt
     8281 attcatgtta tagaataatg atgctatacc cgtgatcatt gaagatgctt gattttgtca
     8341 tgacagagga tcatgctacg caacatgaaa atcttcatct ccgatctaca aaaagcagag
     8401 ctggaacgcc ttcacgatac cagtcgtaac aagcgggtct gcgatcgtat taaagccatt
     8461 cttctggctt ctgaaggctg gagttcagtc atgattgctc aggctctgcg cctgcatcag
     8521 accaccgttg atcatcatat ccatgagttt atgaataaag ggaagttaaa gcccgaaaac
     8581 ggtggttcag acagtaaact cagtgctgaa caaacatcac tcttaatcaa acatttatct
     8641 gataatttgt ttcaccatac tcatgaaatt atcgcctttg ttctccacac atggggaatt
     8701 ttgttcagcg ttgccggtat gaacaaatgg ttgcatcgta acggcttctc ttacaaaaaa
     8761 ccggctggaa cgcctcataa attcagcgaa gagaggcagg cgcaattcat tgaatattat
     8821 gaagacctga aagaaaaagc aggcgatgaa cctgttcttt ttatagatgc tgtccacccc
     8881 actcaggcca ccaaaatcag ttatggctgg atacgaaaag ggcactggaa agcaatagaa
     8941 acaacaggta gccgtacacg gctgaatatc atgggtgcgc taaatctgaa gtctccggaa
     9001 cgcccactca tcagtgagta cccaaccatt aatgcgttaa gcatatcccg gtttttcaat
     9061 gagataagaa aggtttatcc tgattataat caaacagtca acatcattct ggatggtgca
     9121 cgttatcacc atgctcaact ggtaacagac tgggctgaag tcgttaatat caaacgcgtt
     9181 caattagcac ttatctacat aataatagac ctttgtaaaa taactgtata tcattactga
     9241 catacagtct attcattaaa gactttttat aactcgaaac agtttttatt gaaacgccga
     9301 ggttattagc aatatctttt ccacttaa
//
LOCUS       DAZKHK010000166         9180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKHK010000166 DAZKHK010000000
VERSION     DAZKHK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.167"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..451
                     /locus_tag="ACHOS5_004458"
     CDS             <1..451
                     /locus_tag="ACHOS5_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779172.1"
                     /translation="WVDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEV
                     MPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDL
                     RKQRDAIYADSTLSLAQQAAMVKSVERDMKIAVDRFNREYNKKVGVE"
     gene            complement(673..1605)
                     /locus_tag="ACHOS5_004459"
     CDS             complement(673..1605)
                     /locus_tag="ACHOS5_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9779173.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1899..2654
                     /gene="mlaA"
                     /locus_tag="ACHOS5_004460"
     CDS             1899..2654
                     /gene="mlaA"
                     /locus_tag="ACHOS5_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP9779174.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2716..4056)
                     /gene="fadL"
                     /locus_tag="ACHOS5_004461"
     CDS             complement(2716..4056)
                     /gene="fadL"
                     /locus_tag="ACHOS5_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP9779175.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIRQSPAWLTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVAPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            4428..4712
                     /locus_tag="ACHOS5_004462"
     CDS             4428..4712
                     /locus_tag="ACHOS5_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP9779176.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            4893..6203
                     /gene="fadI"
                     /locus_tag="ACHOS5_004463"
     CDS             4893..6203
                     /gene="fadI"
                     /locus_tag="ACHOS5_004463"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP9779177.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            6203..8347
                     /gene="fadJ"
                     /locus_tag="ACHOS5_004464"
     CDS             6203..8347
                     /gene="fadJ"
                     /locus_tag="ACHOS5_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP9779178.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QIRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARTFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPRGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            8550..9035
                     /gene="sixA"
                     /locus_tag="ACHOS5_004465"
     CDS             8550..9035
                     /gene="sixA"
                     /locus_tag="ACHOS5_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP9779179.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2245 a         2184 c         2437 g         2314 t
ORIGIN      
        1 ctgggtggac tatatctccg gagggacagg gcgcttcatt tccaaaacca cggatgcggc
       61 ggtgaaatcg ctgaatggta ttgatatacc ggagcagcag gtgcccttcc tggggaaaat
      121 ttcaggtgag gtgatgccgt atgctgacca gcagaagatg tacgaccgga tgacagaggt
      181 tgcgcagtat cacgcagagc tgaagagtct gaccggtgca gaaagaacgg cgttcattga
      241 cgagaacaac ggaaaattgt cgatgaacgg gcttatgcag gatacccgga agagactgaa
      301 ggatttgcgt aaacagcgtg atgccattta cgccgacagt actctcagtc tggcgcagca
      361 ggcggcgatg gtgaaatcgg ttgagcggga tatgaaaatt gccgtggatc ggtttaaccg
      421 cgagtacaac aaaaaagtgg gagtggagta aagaacatgc cccgtacgga agtgcggggc
      481 atgcttaacg agatgtgtac ataaatgggt actggtatga cgattgtttt ggtattttat
      541 gtatttaaat caatgtgtta atacttacat tcattatatc catttaacta agaggacaat
      601 gcggcatgag tatacccgct aatggactac ggggtaagta cacagccgct cgattgctta
      661 aaccctcgcc atttatgccg ggtttttatc atttttctta atgttttccg cacgttctgc
      721 tttttgacgt gcttctgctt tgcgcttgtt gctcatgtcg ttacgaatct gtgcatgact
      781 cattaacgca aagataaagg tgccgccgca gatgttcccc gctaaagtag gtagtgcaaa
      841 gggccagatg aaatcgctcc agtgcagcgt gccgttaaac accagataga ggatttcaac
      901 agaaccgaca acgatgtggg tggtgtcacc cagggcaata agccaggtca tcaatataat
      961 caccacaatc tttgccgcac ccgctgcagg aaacatccat accatagtgg cgatcagcca
     1021 gccggaaatg atcgcgttgg caaacatctc gctgggggtg ttcttcatca catccatgcc
     1081 gattttgaca aatgcatcgc gagtttcttc attgaagata ggcatatatt caaatgccca
     1141 cgcagcaata cctgtcccga gaatattacc cagcagcacg atgccccata accgcataag
     1201 taagccgacg ttgctcattg tcggtttttg catgacgggt agtaccgcag ttacggtgtt
     1261 ttcggtaaat aattgctggc gggccattat gacgataata aaaccaaagg tataaccgag
     1321 attctccagc aagaagctgc ccggcacgcc ttccagttcg acatgaaata tcccttttgc
     1381 cagtagcgaa gcgcccatcg acaggcccgc cgcaatggct gaccacagta gcgccattgc
     1441 gtcgcgttcc agctcttttt caccatcctg gcggatatgc tcatgaattg ccatcgcccg
     1501 ggaggggagt cggtcttcat ctatttctat ttttttaccg cgctcttttt cttcgctctc
     1561 aacttcaatt tcgtcgctgt gttgatcaat tttgtcgttg tccatggtct cttctcttga
     1621 attagcacgt atagctaaag cgtagcggct tttttgctcg caactggcgg gagttactct
     1681 gaaaatgtag aaaaggccgc gtttgccttt ttctgtttct attgaatcaa gtagcctaca
     1741 gggcggcgat taccaggcta tgatcaaatc agcaaatcag ggcgtctgga catcagttga
     1801 cgtgctgtta caatcgccca cacctaaaca ggcggatacg gtatcgttcc gtcatggatg
     1861 gcaaactgca taagccataa aaaaacaggg agacatttat gaagcttcgc ctgtcggcgc
     1921 ttgctctggg aactacgctt ctggtggggt gtgcgagttc cggtacagat cagcaagggc
     1981 gttctgaccc gttagaaggg tttaaccgca ctatgtacaa cttcaacttc aatgtattag
     2041 acccgtatat tgttcgaccg gtcgctgtcg cctggcgtga ttatgttccg caacctgcgc
     2101 gtaacggttt gagcaacttt accggcaacc ttgaagaacc tgcggtgatg gttaactact
     2161 tcttgcaggg cgacccttat caggggatgg tccactttac ccgctttttc ctgaatacca
     2221 ttttgggaat gggcggcttt attgatgttg cagggatggc aaacccgaaa ctgcaacgga
     2281 ctgaacctca ccgcttcggt agtacgcttg gtcattatgg tgtgggttat gggccgtacg
     2341 ttcagttacc gttctacggt agcttcacgc tgcgtgatga cggtggtgat atggcggata
     2401 gtctttaccc ggttctttcc tggctgacct ggccgatgtc tgtgggtaaa tggacgcttg
     2461 aagggataga aactcgtgcg cagttgttgg attccgacgg tctgctgcgt cagtcgtccg
     2521 atccttatat tatggtgcgt gaagcgtact tccagcgtca tgatttcatc gctaatggcg
     2581 gtgaactcaa accgcaggaa aatccgaacg cgcaagcgat tcaggatgat ttaaaagata
     2641 tcgattctga ataataaaca aacaaaaaag gtgagtcgca aaactcacct tttttcttat
     2701 taagctaacg cgttatcaga acgcgtagtt aaagttagta ccgaacagcc aggctttacc
     2761 ttcagactcg aactgatatg ggccttcgtt aattttcacg ctctgaccgt gcatataaga
     2821 aacaccaacg tcgactgaag catctttatt aaatgcgtaa gtcgtacctg cactcagcca
     2881 gaaacggtcc tggtccggaa tggagataga acgattctgt gccggaactg ggctgtcatc
     2941 aaaggcgata ccggtacgga aggtccagtt atcatcgtag taataagtgg tacccaacgc
     3001 gatgcggtaa gcatctttaa agccttcatg tttctggaac agcgtgtcgc cactcgttga
     3061 ggtcgctttc aactgctgga actgactcca gctggtgtaa gccaggctat agtgaattgc
     3121 ccactgcgga gcaacacggt tataacccga cacttcccac atttcaggca ggttcagcgt
     3181 cagataacct gactgcgttg cgccacctgt cgcggtagga attggcaaac cgtagttatt
     3241 aaaagccggg ttaagatcgc tgctgtagtt acccttgaag tcaattttca cttcagagcg
     3301 gtaggtcaat gcgtagcggt tgtttttatc cagttcatac aggataccgg cgttccagcc
     3361 aaagccccac tggttaccgt tcaggtgagc gattttggta ttactgtcga tgccgttggc
     3421 ggtagctgct aatgcttgcc cctgaggagt cagccaagca ggagattgcc taatctgacc
     3481 agccaccagt tgccccagat cgcctgcgaa acgttcaatt ttcgcgcgag cgtagacggc
     3541 gttgaaacca agaccaaagc tccatgcatt atttaagcga tatgcaccgc ttaagttcag
     3601 gttcatggtt tcgaggtcgg tggtaccacc gacagaaccg cctgcataag tatcgttaaa
     3661 ctctgtcgcc agaccatagt tagaggtaat tgaagccccc caaccaaatt ggtcgttaat
     3721 cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc gcgatattat cggctttcag
     3781 gctacgacca gacggagacg tcccgctgat atttacgtcc ggatctatat aaaccgcacc
     3841 cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg gggttacggc taacgttacc
     3901 tgcatcatcg gcaattgcac cttcccctga ataagcccgg cccaggccag aggaagaaaa
     3961 ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag ataagtgcca ctgcgactgc
     4021 gagagcagac tttgtaaaca gggttttctg gctcatgacc ataacctcaa tgatttattt
     4081 ttatacaaac tatgttacgt gctgtaacag gagcgcgaag tgtagggtct gaggtacatc
     4141 ttagaaatca gaccagtggc gagagtatag gtcggaccag ctggaatgtt gcaagtatgt
     4201 ttctatcttt tttcagatat gatctaagaa acgcgatcca gctggcaaaa ttgggaggtg
     4261 gcgcactcat gaattcgggt gaattttgtt ttagatcatt tttaagtgtg atttcggtca
     4321 cttatccgat tttgcagaat taacaactgg agcagcactt tccggaagcg taaaatatcc
     4381 acaacgttta tctggcacct tgggtgcaca tacagaggaa atcaacgatg agtaaatgta
     4441 gtgctgatga aaccccggtt tgctgctgta tggatgttgg caccattatg gacaactccg
     4501 actgcaccgc gtcttacagc cgtgtgtttg caaaccgcgc agaagcagaa caaacgctgg
     4561 cggcgctgac tgaaaaagcc cgtagcgtgg aatccgaacc gtgcaaaatc accccgactt
     4621 tcactgaaga gtctgacggt gtgcgcctgg atatcgattt cacttttgcc tgcgaagctg
     4681 aaatgctgat cttccagctg ggtctgcgtt aattcctctc gggtacatac gcccctgtca
     4741 gttgctggca ggggcgtttt cttttctctt cttcttttct ctctgtgtca tagctcccac
     4801 tttccctttt ctccacttgg ctaaatgtaa aaaaatggtt aagactatga tcaggtcaga
     4861 ccactttatt tattttttta caggggagtg ttatgggtca ggttttaccg ctggttaccc
     4921 gccagggcga tcgtatcgcc attgttagcg gtttacgtac gccttttgcc cgacaggcga
     4981 cggcttttca tggcattccc gcggttgatt tagggaagat ggtggtaggc gaactgctgg
     5041 cacgcaccga gatccccgct gaagtgattg aacaactggt cttcggtcag gtcgttcaga
     5101 tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg tactggaatg aatgtgcata
     5161 ccgatgctta cagcgttagc cgcgcttgcg ctaccagttt ccaggcggtt gcaaacgtcg
     5221 cagaaagcct gatggcggga actattcgag cggggattgc cggtggggca gattcctctt
     5281 ccgtattgcc aattggcgtc agtaaaaaac tggcgcgcgt actggttgat gtcaacaaag
     5341 cccgcacaat gagccagcgc ctgaaacttt tttctcgctt acgtttgcgt gacttaatgc
     5401 cggtgcctcc ggcagtggcg gaatactcca ctggattacg gatgggggac accgcagagc
     5461 aaatggcgaa aacctacggc atcacccgag aacagcaaga tgcactggcg caccgttcgc
     5521 atcagcgtgc cgctcaggcg tggtcagaag gtaaactcaa agaagaggtg atgactgcct
     5581 ttatcccccc ttataaacaa ccgcttgtcg aagacaacaa tattcgcggt aattcctctc
     5641 ttgctgatta cgcaaagctg cgcccggcgt ttgatcgtaa acacgggacg gtaacggcgg
     5701 cgaacagtac gccgctgacc gatggcgcgg cagcggtgat cctgatgact gaatcccggg
     5761 cgaaagaatt agggctggta ccgctggggt atctgcgcag ctacgcattt actgcgatag
     5821 atgtctggca ggacatgttg ctcggtccag cctggtcaac accgctggcg ctggaacgtg
     5881 ccggattgac gatgagcgat ctgacattga tcgatatgca cgaagccttt gccgcccaga
     5941 cgctggccaa tattcagttg cttggcagcg aacgttttgc tcgcgacgta ctggggcgtg
     6001 cacatgccac tggcgaagtg gacgatagca aatttaacgt gcttggcggt tcgattgctt
     6061 atggacatcc cttcgcagcg accggcgcgc ggatgattac ccagacattg catgaacttc
     6121 gccgtcgcgg cggtggattt ggtttagtga ccgcctgtgc tgcgggtggg cttggcgcag
     6181 caatggttct ggaggcggaa taatggaaat ggcatcagcg tttactctta atgttcgtct
     6241 ggacaacatt gctatcatca ccatcgacgt accgggtgag aaaatgaata ccctcaaggc
     6301 ggaatttgcc tcgcaggtac gcgccattat taagcaaatc cgtgaaaaca aagagttgcg
     6361 tggcgtggtg tttgtctccg cgaaaccaga caacttcatt gctggcgcag acatcaacat
     6421 gatcggcaac tgcaaaacgg cgcaagaagc ggaagtactg gcgcggcagg ggcaacagtt
     6481 aatggcggag attcatgctt tgcccattcc ggttatcgcg gctattcatg gcgcttgcct
     6541 gggcggcggg ctggagttgg cgctggcgtg ccacggtcgt gtttgtactg acgatcctaa
     6601 aacggtgctc ggtttgcctg aagtacaact tggattgtta cccggttcag gcggcaccca
     6661 gcgtttaccg cgcctgatag gtgtcagcac agcattagag atgatcctca ccggaaaaca
     6721 acttcgggcg aaacaggcgg taaagttggg gttggtggat gacgtcgttc cgcactccat
     6781 attgctggaa gccgctgttg agctggcaaa gcaggatcgc ccatcttcac gccctctacc
     6841 tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg ctgctgttca aaatggtcgg
     6901 taagaaaaca gaacacaaaa cgcaaggtaa ctatccggcg acagaacgca tcctggaggt
     6961 tgtcgaaacg ggattagcgc agggcaccag cagcggctat gacgctgaag cacggacgtt
     7021 tggcgaactg gcgatgacgc cgcaatcgca ggcgctgcgc aatatctttt ttgccagtac
     7081 ggacgtgaag aaagatcccg gcagtgatgc gccgcctgcg ccattaaaca gcgtggggat
     7141 tctaggtggt ggcttgatgg gcggcggtat tgcttatgtc actgcttgta aagcggggct
     7201 tccggtcaga attaaagata tcaacccgcg gggcataaat catgcgctga agtacagttg
     7261 ggatcagctt gagggcaaag ttcgccgtcg tcatctcaaa gccagcgaac gtgacaaaca
     7321 gctggcatta atctccggaa cgacggacta ttgcggcttt gcccatcgcg atctgattat
     7381 tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg gtggcggaag tggagcaaaa
     7441 ttgcgctact cataccatct ttgcttcgaa cacgtcatct ttaccgattg gcgatatcgc
     7501 cgctcacgct gcgcgacctg agcaagttat tggcctgcat ttcttcagtc cggtggaaaa
     7561 aatgccgctg gtggagatta ttcctcatgc gagtacatcg gcgcaaacca tcgccaccac
     7621 ggtaaaactg gcgaaaaaac agggcaaaac gccaattgtc gtgcgtgaca aagccgggtt
     7681 ttacgtcaat cgcatcttag cgccttacat caatgaagct atccgcatgt tgaccgaagg
     7741 cgagcgtatt gagcatatcg atgccgcgct ggtgaaattt ggttttccgg taggcccaat
     7801 ccaacttttg gatgaggtag gaatcgacac cgggactaaa attatgcctg tactggaagc
     7861 cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt tcttcaattt tgaacgacga
     7921 tcgcaaaggc agaaaaaatg gccggggttt ctatctttat ggtcagaaag ggcgtaaaag
     7981 caaaaaacag gtcgatcccg ccatttaccc gctcattggc gcacaagggc aggggcgact
     8041 ctccgcaccg caggttgctg aacggtgtgt gatgttgatg ctgaatgaag cagtacgttg
     8101 tctggacgag caggttatcc gtagcgtacg tgacggggat attggcgcgg tatttggcat
     8161 tggttttccg ccatttctcg gtggaccgtt ccgctatatc gattctctcg gcgcaggcga
     8221 agtggttgca ataatgcaac gactggccac gcagtatggt tcccgtttta ccccttgcga
     8281 tcgtttagtt gagatgagcg agcgtgggga aagtttttgg aaaacaactg caactgacct
     8341 gcaataagaa ggtcaaagtt atatgaatcc gcgctgaatg gcggagtttt gctcaaaatg
     8401 taaacgcata ttgactatac ttacgccatt gaggtaaaaa acagcgtttc attcggtgaa
     8461 tggataaggc acaatgccgg ccaccgtttt ctttctctgg tttcagatga aagaaaacgg
     8521 gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt tatcatgcgt cacggcgacg
     8581 cagccctcga tgccgccagt gattcggttc gtcctctgac cactaacggt tgtgacgaat
     8641 ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga aatcgaacgc gttctggtga
     8701 gcccgttcct gcgagccgag caaacactgg aagaagtagg ggattgtctg aacctgcctt
     8761 ccagtgcaga agttctgccg gagttaacgc cctgcggcga tgttggtctg gtcggtgcct
     8821 atttgcaggc gttgaccaat gaaggtgttg cttcggtatt ggtgatctcc cacttaccgt
     8881 tagttgggta tctggttgcc gagttatgtc cgggcgaaac gccgcctatg tttaccactt
     8941 cagcgattgc cagcgtaacg cttgatgaaa gcggaaaagg ccaattcaac tggcaaatga
     9001 gcccttgtaa tttgaagatg gcaaaagcaa tttaagcaat caatgcccgg ttacgtgcgc
     9061 gtacaccggg cagtttctcc gtcatacaat cattcttttt cttgtctaaa tgttgttaat
     9121 atttaacaac tctcgatagt tattatattt tcccattatt gaatcccgta ataaaataaa
//
LOCUS       DAZKHK010000167         9078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKHK010000167 DAZKHK010000000
VERSION     DAZKHK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.168"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            172..648
                     /locus_tag="ACHOS5_004466"
     CDS             172..648
                     /locus_tag="ACHOS5_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9779180.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            645..2768
                     /locus_tag="ACHOS5_004467"
     CDS             645..2768
                     /locus_tag="ACHOS5_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9779181.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVCKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAVDGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQARSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            2765..2977
                     /locus_tag="ACHOS5_004468"
     CDS             2765..2977
                     /locus_tag="ACHOS5_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779182.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2977..4479
                     /locus_tag="ACHOS5_004469"
     CDS             2977..4479
                     /locus_tag="ACHOS5_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9779183.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAVGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4424..6448
                     /locus_tag="ACHOS5_004470"
     CDS             4424..6448
                     /locus_tag="ACHOS5_004470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9779184.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGERALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            6536..6862
                     /locus_tag="ACHOS5_004471"
     CDS             6536..6862
                     /locus_tag="ACHOS5_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP9779185.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     DAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            6855..7136
                     /locus_tag="ACHOS5_004472"
     CDS             6855..7136
                     /locus_tag="ACHOS5_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9779186.1"
                     /translation="MSDLFTRMCCRMDGATVRVMGKQAEINGVVYDVMPEEESADMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            7139..7765
                     /locus_tag="ACHOS5_004473"
     CDS             7139..7765
                     /locus_tag="ACHOS5_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779187.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMTHQLRLVLKRKS"
     gene            7775..8173
                     /gene="gpU"
                     /locus_tag="ACHOS5_004474"
     CDS             7775..8173
                     /gene="gpU"
                     /locus_tag="ACHOS5_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9779188.1"
                     /translation="MKHREIRAAILSALKENISERVTWFDGRPVFIDEQELPAVAVYL
                     TDASATEEFVDEGTWEAVLHIEVFLRAKETDSVLDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEYMMWGSADLQWNITYQM"
     gene            8181..8930
                     /locus_tag="ACHOS5_004475"
     CDS             8181..8930
                     /locus_tag="ACHOS5_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779189.1"
                     /translation="MTLPNPLAPVKGSGTTLWVYTGNGDAWANPLSDDDWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKSVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            8946..>9078
                     /locus_tag="ACHOS5_004476"
     CDS             8946..>9078
                     /locus_tag="ACHOS5_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9779190.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     "
BASE COUNT         2115 a         2158 c         2838 g         1967 t
ORIGIN      
        1 ttgtgatttt atcgtcatca tcatcatgat ggtaacttgt tgtttttaat gctgttagta
       61 ttaaaaagat gatgattgtg gttgtttttt gttcgacatc tttatatggc ggcatttctt
      121 tacaaaaaaa cagagtcact tctgttctgc ggtttatgtg gagggatgta aatggacggc
      181 gagctgaaaa atatgaagtt aaatattaat caactggcag ccctttcagg tctgcaccgg
      241 cagactgttg ccgccagaat ggcggatgtt cctcttgcac caggcagtaa tgaaaagaaa
      301 aaactgtatc tcctgacgga tttgataact tcgttgctgg aaaaaccacc atcttccgaa
      361 gatgaggata tggatcctca cgctcgtaag gcatggtatc agtccgagcg cgagcgtctt
      421 aaatttcagc atgaaactgt tcagcttgtg ccagtcagtg atgtcaggcg gtccttttct
      481 gtcgtggtga aagcgatagt tcaggtactg gaaacctggc ctgaccggct ggagagggac
      541 agggggtgga ctgcatcaca actgaatgaa gtacagattg tggttgatga gatccgcgac
      601 acactggaaa aggcagtcat tgactgttgt gatgaggccg atatgtgaat caggtgaacg
      661 agagccatag ccgcgcatcc gatatctggc gcgaagtggc ctcgctgttt cgcccacctg
      721 gccggttacc agtagcggaa gccatcaggc gttatatgcg ggtaccacgg ggagccaata
      781 cttccggtcc gtgggagtca tcgctgacgc cctatatgat agaccccatt aatacattat
      841 cagcccgtga atatgacgcg gtggtgtttg tcggacctgc gcgaaccggg aaaaccgaag
      901 ggctgattga tggctggatt gtgtacggca tcatctgtga tccggcggac atgctggtgg
      961 tgcagatgac cgagacgaag gcgcgtgagc attccagaac gcgtctttcc aggacgtttc
     1021 gccacagccc ggaggtctgc aaacgcctca gcccctcccg taatgacaac aacgtccacg
     1081 acaaaatgtt tcttgacggc tcattcctga aaattggctg gccgtcgatc accgtatttt
     1141 cctcttcgga ttaccgtcgt gtggcgctga cggattatga ccgtttccct gaaaacgtgg
     1201 acggggaagg ggatgccttc accctggcat caaagcgtac caccaccttt atgtcttcgg
     1261 ggatgaccct ggttgagagt tcaccggggc gggatatcac tgacaccaaa tggcgttgtg
     1321 gtggcgcaca tgaggcaccg ccaacaacgg ggatcctgtc actgtataac aggggagacc
     1381 gccgccggtg gtactggccg tgtccgcact gcggggaata ttttcagccg gtgatggata
     1441 acatgaccgg ttaccggaat aaccctgatt ttgtggctgc cgggcaggcg gcccgtctga
     1501 tgtgtccgca ttgtcgcggg ctgattgctc cggaacagaa gcgtgaactg aataaccagg
     1561 ggatctggct gcgggaaggc gaacgggcag cggtggatgg cagtatcacc ggaacccccc
     1621 gaaattccag aatagcaagt ttctggatgg aggggccggc tgcggcgttt cagacctggg
     1681 aacaactgat ttttaagctg ctggcggcag aagaagagta tgagcgaacc ggcagtgaag
     1741 agaccctgaa agcggtggtg aacaccgata tcgggcggcc ctatctgccc cgttcagcca
     1801 cggaacagcg taaaagtgaa ctgcttgaac agcgtgccga gccgtttccc cggagatctg
     1861 tgccggatgg tgtgcgtttt attgaggcaa cggttgacgt tcagggcggt aaaaatcgcc
     1921 gttttgttgt gcagatcacc ggatacggag agcaggggga acgctggatt gttgatcgct
     1981 acaacatccg gcattcacta cgctgcagtc ccaacggtga aagtctgccg gttgatccgg
     2041 cagcatatcc ggaggactgg gatttgttgc tgacggatgt gttccataaa acatggccgc
     2101 tggcttctga tccggatgtg cgcatgcgtc tgatggccat ggctgtggat acgggagggg
     2161 aagccggggt gacagataac gcctatcgtt tctggcgtcg ttgccggagt gacggactgg
     2221 gcaacagggt gtttctgttc aagggggatg gtcttcgccg tgacaggctg attaaccgta
     2281 ccttcccgga taataccggc agaagtgccc gccgtgccag agccagtggc gatgtcgcgc
     2341 tgtggctggt tcagacggat gcgtttaagg accgtgtaaa taatgccctg tggcgtgaca
     2401 caccagggcc gaactatatc cactttcccg actggctggg gcggtggttt tacgatgagc
     2461 tgacctatga agaacgcggc agtgacggaa aatggcgaaa accgggcagg ggcgctaacg
     2521 aggcgtttga cctgctggtt tatgcggatg cgcttgccgt tctgcatggt tatgaaaaga
     2581 tccgctggcc ctccgcaccg gactgggcac agcgggaaac gtggctcgtc ttcccgcagg
     2641 cgcgttctgg tgaaacggta tccccggaac tgacggccgg ggcagaaaaa cgccgtcgcc
     2701 ggaagaaaaa actgcggacg gagcgtgcgg aagataatcc atggataaca tcaggaggct
     2761 ggttgtgagc acagaagaag ccagagaaat gatacagcgg taccgtgaag cggaaatggc
     2821 cgtactggag gggaagtctg tcaccttcaa cgggcagcaa ctgacgctgg aaagcctttc
     2881 tcagatccgc gccggacgtc aggagtggga acgcaggctt gccgcgatgg tgagccgcag
     2941 gcggggaaaa ccgggattta aactggcgag gttttaatgg caattattga tgatgtgata
     3001 ggcgtgtttt cccccggatg gaaagcagcc agactgcgtt caagggcgtt aatcatggcc
     3061 tatgaggcgg tgaaaccgac ccggacacat aaagcccgac gggaaaatcg ctctgctgat
     3121 cagctcagta aatacggtgc ggtttccctg cgggagcagg cccgttttct ggatatcaat
     3181 catgacctgg tgattggtgt gtttgacaag ctggaagagc gggtgattgg tgccagggga
     3241 attattgtgg agcctcagcc attacgaaaa aacggggaaa tggcggctga gctggctgcg
     3301 gatatccgcc ggttgtgggc tgaatggtcc gtgagtccgg atgtgacagg gcagtatacc
     3361 cgtcccgtgc ttgaacgttt actgctgcgg acctggctgc gggatggtga agtgtttgcg
     3421 cagatggtca gtggtgcggg aaacggtctg gaacggacgg cgggagtgcc attctggctt
     3481 gaggcgatgg agccggattt tgttcccatg cgcactgatg aatccgccgg gctgaatcag
     3541 ggggtttttc ttgatgagtg gggaagaccg aaaaaatatc tggtttataa aaattatccg
     3601 gtcagcggcc ggcagagtga tacgaaagaa atcgctgtcg gaaaaatgat ccacctgaag
     3661 ttcacacgcc gtctgcatca gacgcgaggc tcatccatgt tatcgggggt gctgatgcgg
     3721 atcagtgccc ttaaggagta tgaggatgcg gaactgacgg cagcgcgtat tgccgcggcg
     3781 ctgggactgt atatccgtaa aggtgacgga caggactatg aagatccggg gatcaaagaa
     3841 accgagcggg aagtccatat caccccgggt attatttatg acgatttgcg caagggcgag
     3901 gatatcggca tggtcaaatc tgaccgtccc aatcccaacc ttgaaacttt ccgcaacggc
     3961 cagttgcgtg cagtggcagc tggcagtcgt ctgagttttt ccagtgcggc gcgtaactat
     4021 aacggtacct acagcgccca gcggcaggag ctggtcgagt ccaccgacgg gtatctgatc
     4081 ctgcaggact gttttattgg cgcggtaacc cgccctgtgt accggacatg gctgaatatg
     4141 gtggttgcgg caggtctgct gaaaattccg gcggatgtgg agatgaaaac gctatataac
     4201 gcgacgtatt ccggtccggt gatgccgtgg atcgacccgg ttaaggaagc tgaagcctgg
     4261 agaattcaga tccggggtgg tgcagcgaca gaatctgact gggtgcgtgc cggcgggcgc
     4321 aatccggatg aggtcaaacg tcgccgcaag gctgaaattg atgaaaacag cagactgggg
     4381 ctggtctttg atactgaccc cgtcaacgac aaaggaggaa acagtgccgg aactgaacga
     4441 cagtatcagc gcgacaccga aagccagcat gaagaataaa tcctggttca ggatgcaggc
     4501 tggggggccg ggtgacgcgg atatttatat ttatgacgag attggtttct ggggagttac
     4561 cgcgaagcag tttgtcagcg aactgaatgc tctgggtgat atcacccaca ttaatctcca
     4621 tatcaattca ccgggtggcg atgtctttga aggcatcgcc atttttaatg ccctgaaaaa
     4681 tcagggggcg accattaccg tgtatgtgga tggcgttgcc gcctctatgg catctgtgat
     4741 tgcgatggcc ggtgatacgg tcattatgcc ggaaaatgcc ttcatgatga tccataagcc
     4801 atggggattc agtggcgggg atgctgagga tatgcgcagt tatgccgatt tgctggataa
     4861 agtcgaatcg gtactgttgc cagcctatgc gcagaaaacc ggaaaaacca ccgatgaaat
     4921 tgccgccatg ctggcggatg aaacctggat gtccggtgcc gaatgtctgg cacacggatt
     4981 tgctgaccag gtgacacccg ctgttgaggc aatggcatgt attcagtcaa aacgtacaga
     5041 ggaatttaaa aagatgccgg aatccatccg aaacatgatt actccgccac gcaacagtgc
     5101 cccgcgtgat accacagtga caatccctgc accggcggta acagaaccat caccggtacc
     5161 ggcagtgtct gatgaggcga ccattcgcgc ccgcgttatg gctgagcaga aggcacgcat
     5221 gtcaggcatt aacgatctgt ttgccatgtt cggcggtcgc tatcagacgc ttcaggcaca
     5281 gtgcgtggct gatcctgact gttcgctgga aatggcccgt gaacgtctgc tgaatgaaat
     5341 gggcaaggag tcctcgccga ccaacaaaaa cacaccggcc catatttatg ccggaaacgg
     5401 caattttgtg ggagacggga tccgccaggc gatgctggcc cgtgccggat ttgaaaatgt
     5461 cgagaaggat aacgcctata acgggatgac cctgcgtgaa tgggctcgca tgtcactgac
     5521 ggagcgcggt attggggtgg ccagttataa ccccatgcag atggtcgggc tggcgctgac
     5581 gcacagcacc tctgattttg gcaatatcct gctggatgtg tcgaacaagg ggctgatcca
     5641 gggctgggag gaatcagaag aaaccttcca gaagtggacc cgtaaggggc gcctgtcaga
     5701 cttcaaaaca gcgtatcgcg tggggatggg cggttttggt tctttgcgtc aggttcgtga
     5761 gggggcggag tataaataca tcaccacctc agatcgcaag gagaccattg ctctggccac
     5821 ctacggggag attttctcca tcacccgcca ggccattatc aatgatgatc tgaatatgct
     5881 ggtggacgtg ccgatgaaga tggggcgtgc ggcgaaggca acgattggtg acctggttta
     5941 caaggtgctg acggataacc cgaaactgtc cgacggtaag gcgctgttcc atgccgatca
     6001 caaaaatatt gccaccgggg ggatctccgt ttccggactg gatgcggccc gtcagatgat
     6061 gcgcctgcag aaagaaggcg agcgcgccct gaatatccgt ccggccttta tgctggtacc
     6121 ggtggcactg gagacggtgg cgaaccagac catcaaatcg gccagtgtga aaggggcgga
     6181 tgcaaacgcc ggtgtcatta accccatcca gaactttgct gaggtgattg cggaagcgcg
     6241 tcttgatgcg gcagatccga aaacctggta tctggcggcg gcacagggca ctgacaccat
     6301 tgaagtggcc tggctggatg gtgtggacac gccatacatt gatcagcagg aaggtttcac
     6361 cactgacggc attgccacaa aaattcgtat tgatgccggt gtggcaccac ttgactggcg
     6421 cggtctggtg cgttcgtcgg tggcctgata accgcgttgt cacaatcact gcccgaaagg
     6481 gcttttttta tgcctgaaaa acagccccac aggggctgtc cggagaaaca gcattatggc
     6541 gaaaaatttt gtacaggacg gcactaccat tgaactggtg aatgccggag atcagaccat
     6601 cctgagcggt gctgcggtgg tggtcggcag tatggtggca gtggccatta ccgatattcc
     6661 tgccggtgat gccggtgacg gttttgccga aggcgtgttc ctgctgccca aacagtctgc
     6721 tgacgatatt cagtccggcg cggtggttta tctgaaggac ggggttgtgc agctggctgc
     6781 agacggtgcg gtggctgcgg gggtagcctg ggaaaatgct cctgcaaaca gcgccactgt
     6841 ggcggtaaaa atcaatgtct gatctgttta cgcgaatgtg ttgccggatg gacggggcga
     6901 ccgttcgggt gatgggcaaa caggcggaga ttaacggcgt agtgtatgac gtgatgccgg
     6961 aggaagagtc cgcggatatg ggggcgcttt cgggcagcca gttgtcactg gtggtgtttt
     7021 cagcccggta ccgtccggcc cgtcatgatg ttgttgtgtt tgcggggcgc acactgacgg
     7081 tgacccgtta tgacacgtac aacggtaaac cccggatttt tgtcgaacag gaatgagtat
     7141 ggcaataaaa ggtctggcgc aggccatgaa aaatctggat gcaattgatc gccgtgccgt
     7201 tccccgggcc tccgccacga cactgaaccg cgtggcgggg gccattattg cgaaaacggc
     7261 ctcttcagtt gccagggagc tggccgttcc ccgccgtctt atccgtgccc gcatccggtt
     7321 aagtccggca cgaccggata aggtttatgc aaaggtttac atcaataccg gcaacctgcc
     7381 tgccatcaaa ctgggggagg cccgtgttcg actttcccgc agaaaacgga gaaagaaagg
     7441 acagcgtgcg gccctgaaag ggggcggcag tgtgctgatt gtggggaaaa gacggatccc
     7501 ggacgccttt atcacccggc tggctaatgg acgctggcat gtgatgcagc gtatgccgtg
     7561 ggcatcatcg tccaccggcg cggacagcaa agggaggccg aaacgccacc gtctgccgat
     7621 cgaagtggtg aagattccga ctgccggacc gctggcagaa acctttgaac gtgaacggga
     7681 ccggatgtac cgggaaaaat taccggcgca gatgatgacg gccatgacgc atcagttacg
     7741 cctggtgctg aaacgaaaat cgtaaggagg gaatgtgaaa cacagagaaa ttcgggcggc
     7801 aatattgtct gccctgaaag aaaatatttc tgagcgggtg acctggtttg atggtcgtcc
     7861 ggtatttatt gatgaacagg aactgcctgc ggtggcggtt tatctgaccg atgcttctgc
     7921 gacggaggag tttgttgacg aggggacctg ggaggccgtg cttcatattg aggtgtttct
     7981 cagggcgaaa gagacggact ccgtgctgga tatgtggatg gaagaaaaaa tccttccggc
     8041 gctggaggcg gttcccgggc tcagtgcatt actgctgaag atgaatcttc aggggtatga
     8101 ctatcgccgg gatgacgaat atatgatgtg gggatcggct gatctccagt ggaatattac
     8161 ttaccagatg tgaggacgtt atgacgctcc ctaatcccct tgcgccggta aaaggttccg
     8221 gcaccacgct ctgggtttat accggaaatg gcgatgcctg ggcaaacccg ctgtcagatg
     8281 atgactggac gcgcctggca aaaataaagg atctgacccc cggcgagatg acggcggaat
     8341 cctacgacga taactatctg gatgatgagg atgctgactg ggtatccacc gggcaggggc
     8401 agaaatccgc cggtgacacc agttttacgc tggcctggaa gcccggcgag aaagggcagc
     8461 gtgatttgat tgcctggttt gacagcagcg agacccgggc ctacaaaatc cgcttcccga
     8521 acggcacggt ggatgtgttc cgtggctggg tgagcgccat tggtaaatcc gtgaccgcca
     8581 aagaagtgat cacccgcact gtgaaaatca ccaatattgg tcgtccgtcg ctggcggagg
     8641 atcggggaga aatcacaccg gtcaccggta ttaccgtgac gccactaacg gggaatgtgg
     8701 caaaaggtca gaatatcacc ctgaccgtgg ccgttcagcc ggaaggcgca acggataaaa
     8761 cgttccgcgc aacctcagcg aatcagaatt tcgccaccat taccgtgaag gggaacacga
     8821 tcaccgtgaa aggtgttgcg gcaggtaaag cgcagatccc tgttgtcacc ggcaatggtg
     8881 agtttgcggc ggtggcggag atcaccgtca cggatggcgc tgcgggctga ggggggagat
     8941 aaagcatgtt tctgaaaaca gaacaatttg aatataacgg tgtgtccgtc acgctgtcgg
     9001 aactgtccgc gctgcagcgg tttgattata tgaagtttgt ttcagacgca gcacaacagg
     9061 agacaacgga gcatgatg
//
LOCUS       DAZKHK010000168         8944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKHK010000168 DAZKHK010000000
VERSION     DAZKHK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.169"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..860
                     /gene="gadX"
                     /locus_tag="ACHOS5_004477"
     CDS             36..860
                     /gene="gadX"
                     /locus_tag="ACHOS5_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP9779191.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1229..1957
                     /gene="gadW"
                     /locus_tag="ACHOS5_004478"
     CDS             1229..1957
                     /gene="gadW"
                     /locus_tag="ACHOS5_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP9779192.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2320..5433)
                     /gene="mdtF"
                     /locus_tag="ACHOS5_004479"
     CDS             complement(2320..5433)
                     /gene="mdtF"
                     /locus_tag="ACHOS5_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP9779193.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5458..6567)
                     /gene="mdtE"
                     /locus_tag="ACHOS5_004480"
     CDS             complement(5458..6567)
                     /gene="mdtE"
                     /locus_tag="ACHOS5_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP9779194.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6675..6953
                     /locus_tag="ACHOS5_004481"
     CDS             6675..6953
                     /locus_tag="ACHOS5_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779195.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6954..7481)
                     /gene="gadE"
                     /locus_tag="ACHOS5_004482"
     CDS             complement(6954..7481)
                     /gene="gadE"
                     /locus_tag="ACHOS5_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9779196.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8280..8852)
                     /gene="hdeD"
                     /locus_tag="ACHOS5_004483"
     CDS             complement(8280..8852)
                     /gene="hdeD"
                     /locus_tag="ACHOS5_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP9779197.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
BASE COUNT         2401 a         2076 c         2080 g         2387 t
ORIGIN      
        1 aaacagtaat atgtttatgt aatattaagt caactatgca accactacat ggtaattgtc
       61 taattgcgta cgcaagacat aaatatattc tcaccatggt taatggtgaa tatcgctatt
      121 ttaatggcgg tgatctggtt tttgcggatg caagccaaat tcaagtggat aagtgtgttg
      181 agaatttcgt attagtttca agagacacgc tttcattatt tctcccgatg ctcaaggagg
      241 aggcattaaa acttcatgca cataaaaaag ttccttcatt actcgttcat cactgtacca
      301 gagatattcc tgtttttcag gaagttgcgc aactatcgca gaataagaat cttcgctatg
      361 cagaaatgct acgtaaaagg gcattaatct ttgcgttgtt atctgttttt cttgaggata
      421 cgcagtttat accgttactt ctgaacgtgt tgcaaccgaa tatgcgaaca cgagtttgta
      481 cggtcatcaa taataatatc gcccatgagt ggacattagc ccgaattgct agcgaattgt
      541 tgatgagtcc aagcctgtta aagaaaaaat tgcgcgaaga aggtacgtca tattcacagt
      601 tgcttactga gtgtaggatg cagcgtgctt tacaacttat tgttatatat ggggtttcaa
      661 ttaagcgagt tgcagtatcc tgtggatatc acagcgtgtc gtatttcatt tacgtttttc
      721 gaaattatta tgggatgacg cccacagagt atcaggagcg atcggcgcag gaattaccga
      781 actgtggctc ggcggcaagt atggctgcgc aagggatttt ttacggcact gaccgttctg
      841 cggaaggaat aagattatag agttttactc agacataaaa aaaacccggc ataggggacc
      901 gggaagagga tagtctgccg tctccagact aataaaccgt tataacactc cctgttggca
      961 cgggaaactt tgtgctctca gtaagttaaa tataactttt actggaaata agatcagcca
     1021 tttttttata aacataagct atacgctgtg tgaaaatata atcgtgttgc atttacttat
     1081 tatcaattaa ctgttatgca aaactacttt gtggataaaa tttggtccta ccaaatatgg
     1141 cagtttttgc acacagaaac agtctggcgt catttcatta gtatactgac attgaaataa
     1201 tcgcagtaat gaaatataag ggatagtcat ggctcatgtc tgctcggtga tcctcgttcg
     1261 tcgttcattc gatattcatc atgaacagca aaaaatatcg ttgcataacg agagtatcct
     1321 gctgctggat aaaaatttgg cagacgattt tgcgttttgt tcactggata cgcgacggct
     1381 ggatatcgaa gagctgacag tttgccatta cttacaaaat attcgtcagt tgccacgcaa
     1441 tttaggattg catagcaaag accgtttgtt aattaaccag tcacccccca tacagctggt
     1501 gacggcgatt tttgatagtt tcaatgaccc ccgggtcaat tcgccgatac tgagcaaaat
     1561 gctctatctt tcctgtttat caatgttttc tcataagaaa gaactgatcc ccttactttt
     1621 caatagtatc agtactgttt caggaaaagt tgaacgcctt attagctttg atatcgctaa
     1681 acgttggtat ctacgcgata tcgcagaaag aatgtacacc agcgagagtc tcatcaaaaa
     1741 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcctcca ggatgtcgat
     1801 ggccagacga ttactcgagt tacgtcaaat acctctgcat actattgcgg aaaaatgtgg
     1861 ctatagcagt acgtcatact ttataaatac atttagacaa tattatggtg taacgccaca
     1921 tcagttttcg caacattcgc cgggtacctt ttcctgacat atttcgcatt tgaatattgg
     1981 tcaggatctc acgtctgctt catgtgaaac tctttcctga tgatttctgc cgggctaccg
     2041 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     2101 catgtttggg attattaagc taacaattca tacgattacg ggaatgtggg tcagtattgt
     2161 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     2221 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     2281 ggctgaaccc tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     2341 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     2401 ggcaaacatc ccgcccatta cgccggtacc taccgcgttt tgcgcgccag agccggcacc
     2461 atgactgata accagcggca ggacgccgag aataaaggcc agagaagtca tcaggattgg
     2521 gcgtaaacgc atacgcgctg cttcgatgat tgcctctacc ggcgttttcc cttctttctg
     2581 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     2641 ggtcagcaaa ccaacctgga agtaaacgtc attacttaag ccgcgcagat cggtggccag
     2701 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     2761 ttcataaagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat atagcgcggg
     2821 agcctgattt gaggataacg cttcctgata cgacagcccg gtccatgagt agccgacgcc
     2881 tgccggaagt tttgcgacca ggtctgccat aaatttcatg gcgtcaccgg tacttttccc
     2941 ggcagccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccagtcgcgg
     3001 tgaaccatag gtccattcgg tagacgaata ggcagaaagc ggtgccatcg tgccagaggc
     3061 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     3121 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     3181 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccatagcct cggctttcgc
     3241 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccgat
     3301 gacctgattc ggtgattgcg ctgccagtga taacagctcg tttcgcgcct gggtcagctt
     3361 ttcgtgcccc agattgccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     3421 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttagcgc
     3481 aatcattgcc cgctgaataa tcgcggtaac cgagttttcc tcgccgacac gttcagacca
     3541 cggcttgagg ctgataaacg ccagaccgtt gttttgcccc tgaccactga agccaaagcc
     3601 accaacggta aacaccgact gaacattatt tttctctttt gtcagataat aatccgtcac
     3661 ctgttgtagc actttcgtgg tgttaaccat ggttgcaccg gaaggtaact gcgcggtggt
     3721 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggcgtacgca ggaacagcac
     3781 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     3841 cgagcgggtg ctgtcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     3901 gaacagggcg ttaggtttgt gaccgccctc cggcgcggct ttcagaatgg tggcgcacag
     3961 ggcaggggtc aggctcattg ctacaaatac tgaaagcagc atggaggaga tcagcgtgat
     4021 ggagaactgg cggtagatct ccccggtcgc accgctcata aaggccatcg gcataaacac
     4081 tgcggaaaga acaacggcaa tcccgaccag cgcacgttgg atctgcccca tcgatttatg
     4141 cgtcgcttcc ttcggcggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     4201 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     4261 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     4321 ggcaatcgtc gggatgattg tggcgcggaa attctgcaaa aacagataca tgaccaggaa
     4381 gacgaggatg atagcctcaa ccagcgtttt gaaaacttcc tgaatagaaa ttttgataaa
     4441 cggtgtggtg tcgtaaggat aaaccgtctt caggcttgca gggaaataag ccgataaacg
     4501 gttcagctcc tctttcaccg ctcgcgaggt atccagggcg tttgctccgg tagccagttt
     4561 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     4621 cccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     4681 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     4741 gttcagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     4801 gttttgcacc ttaatctggg aaataacatc ggaaggtacc aggttatatt tattgagttt
     4861 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     4921 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtctg cgatatcgta
     4981 ctggttgagg ctgccgttat cagaaataaa tgccgctacc atcaggatat tactgctcga
     5041 cttatcgacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     5101 cagtttattt tgcacctgaa cctgtgcgat atcaggcgat gtccccgtct cgaaggtcag
     5161 agtgatggag gcattacctg ccgcatcgct ggttgaagac atgtacatca ggccatcaag
     5221 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     5281 acccggatag gttgcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     5341 cggtaagttc atgatcgcca gaccgcctgc aagcatcata ataatggcaa gtacccaggc
     5401 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     5461 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     5521 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     5581 gacaacccac tggtcgccga tggctttgct ggcttcgatt tcgcgtagct gcacgacatc
     5641 gtctttatcc agaatgagtg ccgttgcttt accctgggcg ttgtgggtga cgccttcctg
     5701 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga catacatgcc
     5761 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     5821 ttcatccacc gtcgggtcgg agaatttcag cgtgccggtc tggccgtaac gtttaccatt
     5881 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     5941 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     6001 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     6061 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     6121 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcaa
     6181 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacatag ttggtcttca gcaacgatgc
     6241 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     6301 tttggcggag ttcagctcag cctgtaaagg cgcaggatca atctgataca gcgaatcgcc
     6361 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataattccgc ccacctgggg
     6421 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     6481 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     6541 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagttt
     6601 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttc tttaaattgc
     6661 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     6721 agatgggatg caagcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     6781 ttctgccaac agtttctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     6841 accaggggaa taaagtcgtt ttcgcctctc ctttagtaat caccgggggt aatgaaatca
     6901 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     6961 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     7021 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     7081 ctttcatttt tgtttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     7141 agttgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     7201 ctttctttac ggctgacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     7261 ggtgccaaaa caataattct ctcggcatct aatttctcca ggaatttaat cgcttcttca
     7321 tcaaggatat ggttgtgata ggtgtcaatg atcaccttga agggtttatt gcctactttt
     7381 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     7441 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     7501 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     7561 atagtaaacg cctataagaa ctttgccctt cgtgaactaa tacgtctatt aacactaatc
     7621 aatacttcag atttatccat cacgcactcc atatattggt cctaagtaag attcctggtt
     7681 gttatcagtt tgtaactagt aaacaaaagg ttaacttttg cggattactg cccaagaata
     7741 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     7801 atttaaatat cgagtctcct tgtttcgact taggctggca attggattgc cagctttctt
     7861 ttaacgcatt gaaattacct gattaaataa ctcattcatg tcttcaggtt tttatatagc
     7921 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     7981 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     8041 cagcactttt aaaacattaa acctgaccac ggaaaatatc agccatgata atgtttgaaa
     8101 tggctaattt gccatggaac gaaaaaaatc agattaaatt cagtaggttg aaataatcaa
     8161 cagcaggcaa ttgtttcaat gatagtgcgt aattgaactg aaacactgta tagctaagag
     8221 agtattaact ggatcatagt cacttaatgt gactatgatc caggtggaaa tagggataat
     8281 tattgctttt taacgaacaa actggcgaag ctgaacagac tggcggcgct aaatatcagt
     8341 tcaattccca ccagtgtgga aaccagcgtt accgacacca tcggcgtagc accaaggaat
     8401 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccaact gcctttcatt
     8461 gaacgctgac gataccaact catcaggcga ataacccctg ccacacagaa caaaccggca
     8521 ataaatgccg caatggcaaa aatgcccagc tctggtgcgc ggatgaagaa atagccgatc
     8581 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     8641 ttgctgaata gcccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     8701 gtgcttaaaa tgtcgccaga gacgaacgga aaactgatac acagcaaccc gacgataaac
     8761 agcagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     8821 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
     8881 atcccctgct atcaatctat gccaaaaacg cgtgtaagaa tgcagtcgat ttaataaaaa
     8941 tttc
//
LOCUS       DAZKHK010000169         8939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKHK010000169 DAZKHK010000000
VERSION     DAZKHK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.170"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..319)
                     /gene="uspD"
                     /locus_tag="ACHOS5_004484"
     CDS             complement(<1..319)
                     /gene="uspD"
                     /locus_tag="ACHOS5_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9779198.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDL"
     gene            complement(346..645)
                     /locus_tag="ACHOS5_004485"
     CDS             complement(346..645)
                     /locus_tag="ACHOS5_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP9779199.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(857..1297)
                     /locus_tag="ACHOS5_004486"
     CDS             complement(857..1297)
                     /locus_tag="ACHOS5_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9779200.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            1398..1997
                     /locus_tag="ACHOS5_004487"
     CDS             1398..1997
                     /locus_tag="ACHOS5_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1454 family protein"
                     /protein_id="HFP9779201.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            2105..2872
                     /gene="tpiA"
                     /locus_tag="ACHOS5_004488"
     CDS             2105..2872
                     /gene="tpiA"
                     /locus_tag="ACHOS5_004488"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9779202.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(2927..3682)
                     /gene="cdh"
                     /locus_tag="ACHOS5_004489"
     CDS             complement(2927..3682)
                     /gene="cdh"
                     /locus_tag="ACHOS5_004489"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP9779203.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(3789..4778)
                     /gene="sbp"
                     /locus_tag="ACHOS5_004490"
     CDS             complement(3789..4778)
                     /gene="sbp"
                     /locus_tag="ACHOS5_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP9779204.1"
                     /translation="MKKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(5098..6060)
                     /gene="pfkA"
                     /locus_tag="ACHOS5_004491"
     CDS             complement(5098..6060)
                     /gene="pfkA"
                     /locus_tag="ACHOS5_004491"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9779205.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(6241..7143)
                     /gene="fieF"
                     /locus_tag="ACHOS5_004492"
     CDS             complement(6241..7143)
                     /gene="fieF"
                     /locus_tag="ACHOS5_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP9779206.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(7894..8565)
                     /locus_tag="ACHOS5_004493"
     CDS             complement(7894..8565)
                     /locus_tag="ACHOS5_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP9779207.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGIGRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNEMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHIRQRTMKKDLESLSSADEILAYRV
                     GWAQE"
     gene            complement(8607..>8939)
                     /locus_tag="ACHOS5_004494"
     CDS             complement(8607..>8939)
                     /locus_tag="ACHOS5_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779208.1"
                     /translation="DPGPRGERGETGPMGPPGASGDDGKSRVMGFRLGNPVTYELQEG
                     DHGWSVNLADGSVITGLSGNTQGGVSRVTTIYYRPLQMTFDGSHWRTVGVGEYVSSGA
                     PGREYFSL"
BASE COUNT         2297 a         2448 c         1992 g         2202 t
ORIGIN      
        1 ggaggtcgca ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt
       61 caatacgcag ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat
      121 ccgacttatt cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat
      181 acaactcgct taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg
      241 cgagctccag ggctttattc accagtaagg catcttcttc attcccggaa attgccacgc
      301 caatgtgttt ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg
      361 cagtgataac tcaaaaataa tcatctcggc ctggcatgag aaggtgaagg ccgcatcaag
      421 ctcaacttgc ccttccagat ccttaatctt ataggccacg ctggcccctt catctgcaga
      481 ggatgcagcg gcggcaagct ctttcagttt ggtcataaag gactctgctt cttcgcgggt
      541 ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc
      601 aatcgcacat cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat
      661 attcgcctct ctgtcgatta cgacttaaga ataatgcgcc gttaaaggaa gaacaaacaa
      721 gtaatgatct tatggattac catttttttt gatattgctt aaggtgaaga gttcagaagg
      781 attttccggg cattgtcact tgcatcgtgg aaataacaat cccggaaaaa aatagcgata
      841 agagatatat taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctggg
      901 tatctttacc atatttattt tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca
      961 tcatcaccag aatcaatgtc gggacaaaac gccccaccgc ccattgccag acacccggta
     1021 aaattgccca gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc
     1081 cggaacgccc gcgatcatgc aagcgcttaa cggttactgc cgctgttggc caaagcaagc
     1141 acacaaggca aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa
     1201 gcaccagcat gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac
     1261 gccctttaaa tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt
     1321 ttccaggatt ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat
     1381 aacaaaaggt actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat
     1441 taaccgttac aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc
     1501 tgctggctgg agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca
     1561 gccagaatcc cagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag
     1621 ccaatctcac tcgtgcagca agtaaaatta acgagaactt gtatgcttct actgcgctgg
     1681 agcgcggtac cttaaaaatc aaaagcattc aaataacctg gctacccatc caggggccag
     1741 agcaaaaagc cgcgaaagcg aaagctcagg catacatggc agcggtgatc cgcacactca
     1801 ccccattaat gaccaaaact caaagccaga aaaaactgca gtcgctacta acggcgggga
     1861 aaaacaaacg ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaacg
     1921 gcgaaaaggg gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg
     1981 aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc
     2041 acagacctgc tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat
     2101 taaaatgcga catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt
     2161 tcacgagctg gtatctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc
     2221 aatcgcacca ccggaaatgt acatcgatat ggcgaagcgt gaagctgaag gcagccacat
     2281 catgctgggt gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacctc
     2341 tgctgctatg ctgaaagaca tcggcgcaca gtacatcatc atcggccact ctgaacgtcg
     2401 tacttaccac aaagaatctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca
     2461 gggactgact ccggttctgt gcatcggtga aaccgaagcg gaaaacgaag cgggcaaaac
     2521 tgaagaagtt tgtgcacgtc agatcgacgc ggtactgaaa actcaggggg ctgcggcatt
     2581 cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac
     2641 tccggctcag gcacaggctg ttcacaaatt catccgtgac cacattgcta aagttgacgc
     2701 taacatcgct gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc
     2761 agaactgttt gctcagccag acatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc
     2821 tgacgctttc gccgtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca
     2881 ggtgccggat ttcatatccg gcacttactt tcctaaactc ctcgccttaa cgcaaaatct
     2941 cacactgatg atcctggatt tcttcggctg aagcacggtt cagcgtcagt agatttcgtt
     3001 gtgtcgccag caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc
     3061 ccatatgttc ccgcgcctca ggcacttctt ccgccagcat cataaatggg ctgcgttgta
     3121 ccagttcgct ttccgttacc cgacgcgcca ggtactcatg cccacgcaaa ccacctggca
     3181 gtggcaacca gcggctactg atgttcgcca gattgttatc cagctgttcg cgcacatcag
     3241 gacgaataca agaaatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga
     3301 tcgccagaga taccgcgcga tcgggaacag gctggccgta tttttggctc ataaaatcac
     3361 gcgcctgcca ggctaaccag aaaaaattag gcgttgacgg atcggtcaac aatgggcttt
     3421 cagtaccgtt aatacggtac gttggcatca acagatattg cagtgggcca tgacgatctt
     3481 ttaaaaccac gtatccggcg ttgggtttga cttccgcaca tggcgacggg ttttggtttt
     3541 cctgctggtt gggcaaacat tgctcaagaa ctatcttacg taacgtgtct gactcttcac
     3601 cggttaattt ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa
     3661 aaagaagacc cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac
     3721 gcaaaatcgt gacaaataaa aaacccagtc gcgagaaacc accgggcttg ataattatcc
     3781 tggggaaatc agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa atgctctttt
     3841 tgcgctttcg tccagccgcc gaactcttca tcaatggtga ataacttcaa ttttggaaac
     3901 gcattttcgt actttttcgc tacctcagcg tcgcgcggac ggtagtagtt tttcgctgca
     3961 atttcctgac cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg
     4021 ccttttttct cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca
     4081 ctcggcgtga cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttca
     4141 ttttcccagg caatcagtac atcgccaatt ccgcgctcga caaaagtgtt agtggatccg
     4201 cgcgcgccag aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct
     4261 tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc aggctgccag gtagttccag
     4321 cgcgcgccac cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga
     4381 tcgttccagt catggatctg cttcggatta cccttacgta ccaggaaaac aatggtggaa
     4441 gtgtacggtg cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccgcgt
     4501 tccgcaattg cgtccacgtc ataggccaga gccagagtga caacatcagc ttcaataccg
     4561 ttgattaccg acgtcgcttg tttgcctgaa ccaccgtgtg actgacgaat caccacgtta
     4621 tcgccagttt gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg
     4681 cgcgttggat catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc
     4741 gccagcaaaa atgttaaccc tacgccccac tttttcatcg cccgactctc ttatgttgtg
     4801 ttgtgatgag caaagcgtgc cagaaggttg accaaacatt aaagaataaa aaaagattgg
     4861 ctataacttg cgagtatatg ttgggggatt aaaaaggcgg aagaaatccg cctcatattg
     4921 ctgacaaagt gcgctttgtc catgccggat gcggcacaaa cgccttatcc ggcctacaaa
     4981 agtttgcaaa ttcaatagat tacagaattc atgtaggcct gataagcgaa gcgcatcagg
     5041 cctttttgct tctgtcatcg gtttcaggct aaaggaatct gcctttttcc gaaatcatta
     5101 atacagtttt ttcgcgcagt ccagccagtc gcctttgaac ggacgcttca tgttttcgat
     5161 agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgg atacctacgc aacgaccacc
     5221 gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc
     5281 gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg
     5341 accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat
     5401 cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc
     5461 ttcacggctg aattcaactt ccggaaccac aacgaattca cagccgccgg caatagccgc
     5521 agccaacgtc agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg
     5581 agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa
     5641 gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcaggccgat
     5701 gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgccgat
     5761 aaccaccagc gcgtcgatcc cacgtttttt caggttttcg atagccacgg cgcggatgtt
     5821 ctcgtcgcgg aattccggga aacgcgcaga accgaggaac gtaccaccac ggttgatcat
     5881 gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc
     5941 gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat
     6001 tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat
     6061 gactacctct gaactttgga atgcaaaatg aaatctgttg ccggaagtct tcttgcacat
     6121 cgaaatgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag
     6181 gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga
     6241 ttatgaaagc atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat
     6301 aatcacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat
     6361 atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt
     6421 cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac
     6481 gatatcaata atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac
     6541 cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgatcc ccaatgcaaa
     6601 cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc
     6661 gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt
     6721 ccggcgcacc acccaacgct gaaacgagac aaggataatc gtacaaatta gcgccacaat
     6781 tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatacca gatgttgaat
     6841 acccgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc
     6901 gagggactca gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata
     6961 tcgcaccacc agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc
     7021 ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcag
     7081 cgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt
     7141 cataaatact cccgctatca actgacggta gtataacgga agcaaatcat ctgcaatgca
     7201 ttaagcagca ggcaaattga ggataaaaaa aacccccaca tcatgtgggg gaagacaggg
     7261 atggtgttct tttatgtgta gtatcctgtt gaaatataat gacttcatat ttcaaactgt
     7321 ccaccaaacg tccacatcga ccacccaaag ccccttatca ggggcttatt ttattcgtga
     7381 taaaaatcgt catgaccatc ccttcctctg aagcatacaa ccgggcccgt gcaaaatacg
     7441 gacttccgga aatctgaaaa attactctta acagagaaga gtacaggaac acgatcggca
     7501 tcactgtttc tgcaaacttc cctgtggcag tctttatttg ccatataaca tagcactgaa
     7561 tgtcttcatt gattttttca ttgtagttac tccttccttg taccgggggt tggtcatgcc
     7621 ccccgttttt tattcccctt gttcccttaa tcgtactgcc aggcgccaca acacacatca
     7681 actgtggctg gtatggaaac accaaaaact ctcacttccg gaaatctgaa aaatcatcca
     7741 tatcaacagg atgctctgga aatgagaccg gcatcacttt caggattaac ttttccgtgg
     7801 cactctttat ctgtgtaggt aaggtttctt cacaataatt acataatcaa cgcagttcat
     7861 gggggaggtg atggtccccc atctttttgt ctgttattcc tgtgcccagc caaccctgta
     7921 tgccaggatc tcatccgcac tgctcagcga ttccaggtcc ttcttcatgg tgcgctggcg
     7981 aatgtggatt tccatccctt tagtgaacat cgcctgctct gccgcttcac tcagcgcaat
     8041 aagctcttct gctgtcaccg gcacatcatt gttttccgca tccgtccaga aaaacgcctc
     8101 cggcagtttc cccgctttcg ctgccgccac cgacggctca agacgcgtct gcgttgactt
     8161 cccgtagtcc catttacgcc cattgtgctc aaacgtgtag ttcgccgctt ccatctcatt
     8221 acgccaggcg ttaatttcat cgcccttcat cccgcgcgct ttctccgcgg tcagcaggtc
     8281 cgtgattttc tccccgtcaa aaccccagcg ccccgagatg tcagctttgc ggttttcttc
     8341 cgtatccgcc acctccacca cactctggcc cactggccac agaactgacg ccaccctgcc
     8401 gatgccggta atgactccgg cactgttata aaccaccttc agcgtctctg ctgaaaataa
     8461 cgactgacac tcataccagt cctgaccatc ctctgatttc aggtacatcg cacccggcac
     8521 atccggattt tccggcgtgt attgtctgaa attctttatg tgcatctgta agtcctcccg
     8581 gttacatatc tgacaggtga tgcgctttat aatgaaaaat actcacgccc cggcgctcct
     8641 gacgacacat actcaccaac accaacggtg cgccagtgag agccatcaaa cgtcatctgc
     8701 agcggcctgt agtaaatcgt tgtaacacgg ctgacgccgc cctgagtgtt ccccgaaagc
     8761 cctgtaatga ctgaaccatc agccagatta actgaccagc catggtcccc ctcctgcaac
     8821 tcatacgtta ccggattccc cagcctgaag cccatgacac gactcttccc gtcatctcct
     8881 gatgcccccg gcggccccat cggcccggtt tctccacgct cacctctggg accggggtc
//
LOCUS       DAZKHK010000170         8884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKHK010000170 DAZKHK010000000
VERSION     DAZKHK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.171"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..663
                     /locus_tag="ACHOS5_004495"
     CDS             <1..663
                     /locus_tag="ACHOS5_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779209.1"
                     /translation="IAAFWFWLIMFHTVPEKLKLNNESEFNSALKGVIVMKDNPMTII
                     PSSHETRLNVIGKWINALILYGEADRIISIMHQNWLGLEKRNEAKWLKKNTEQLKWAW
                     EYIDARISPTYLSWFNPVNDNERYIAIVVLLKMLFPTDTPCLLTPKEFHLGFAAKERF
                     FDKMHNAFRKQFIDGKKDKRVQINVKISPSAKSALDRLTRERKTTQQAILEQLILYGR
                     LD"
     gene            complement(660..1610)
                     /locus_tag="ACHOS5_004496"
     CDS             complement(660..1610)
                     /locus_tag="ACHOS5_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779210.1"
                     /translation="MASLSINTLRRMSVCKSQAWKDTQRILKRSGLVYRGERSESFDP
                     EKHFNRYTVRYLYLLNIIALKIRSVSKFSCWIEVGQCHRMTGKHLSLNAPPFMLIPRS
                     IRRKVDGFRQSKGEATPKSAQPFTGSLYEVLSKESDSAKLDAWIAELPLNLQEEGKEI
                     SVMGFSPRAVARPVLRSASPTFELFYQEYQSLRLKSVFKFEPRFEQLLTRLSFRKQSI
                     LRKQQYRVKSQLLERLGNVLFFTSLYNQGCLEEFINALVEKEDIYLKMSPGEEKVKAH
                     QKMVNYIEAFCNKMTEKYLMPAASRHYKKKKIAKSESGES"
     gene            1716..2600
                     /locus_tag="ACHOS5_004497"
     CDS             1716..2600
                     /locus_tag="ACHOS5_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9779211.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAGEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGETIGKSFDDAVARFSFPAWMLQLLR
                     KTRDRIIHLLVTLWDHLF"
     gene            2719..3396
                     /locus_tag="ACHOS5_004498"
     CDS             2719..3396
                     /locus_tag="ACHOS5_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779212.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEAGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTPAINKDSVLQ
                     AARQAYARKYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            3402..3635
                     /locus_tag="ACHOS5_004499"
     CDS             3402..3635
                     /locus_tag="ACHOS5_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9779213.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            3726..4544
                     /locus_tag="ACHOS5_004500"
     CDS             3726..4544
                     /locus_tag="ACHOS5_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9779214.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEDKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALIYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4636..5121
                     /locus_tag="ACHOS5_004501"
     CDS             4636..5121
                     /locus_tag="ACHOS5_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9779215.1"
                     /translation="MTTVTHNSTTSSVSVTAASGNNPPQLVATLVPDEQRISFWSQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDKTWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            5137..5613
                     /locus_tag="ACHOS5_004502"
     CDS             5137..5613
                     /locus_tag="ACHOS5_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9779216.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIASETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQTLALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            5676..5897
                     /locus_tag="ACHOS5_004503"
     CDS             5676..5897
                     /locus_tag="ACHOS5_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9779217.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            5977..6345
                     /locus_tag="ACHOS5_004504"
     CDS             5977..6345
                     /locus_tag="ACHOS5_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9779218.1"
                     /translation="MSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVKLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            6435..6812
                     /locus_tag="ACHOS5_004505"
     CDS             6435..6812
                     /locus_tag="ACHOS5_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9779219.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLSRLLDKHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNDITQGKHPEAKQ"
     gene            6809..6958
                     /locus_tag="ACHOS5_004506"
                     /pseudo
     CDS             6809..6958
                     /locus_tag="ACHOS5_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            7034..7231
                     /locus_tag="ACHOS5_004507"
     CDS             7034..7231
                     /locus_tag="ACHOS5_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9779220.1"
                     /translation="MMPLTTETALDILIAWLQDNIDCESEIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLQQNR"
     gene            7316..8158
                     /locus_tag="ACHOS5_004508"
     CDS             7316..8158
                     /locus_tag="ACHOS5_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9779221.1"
                     /translation="MSEITAPRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLTRQ
                     LAEISAMTESIGGKTVLDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARETWYRSLEYDNFPEISEANILSTFKQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIINNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIRSVQGKECYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     VLPS"
BASE COUNT         2285 a         2290 c         2111 g         2198 t
ORIGIN      
        1 attgcagcat tctggttctg gttaattatg tttcatacag ttcccgaaaa acttaaactt
       61 aataatgaat ctgagttcaa ctctgccttg aagggggtga tcgttatgaa ggataacccc
      121 atgacaatta ttccttcaag tcatgaaaca cgactgaatg ttataggaaa atggattaat
      181 gcgttaatcc tttatgggga agcagatcgg attatctcaa taatgcatca aaactggctg
      241 ggactcgaaa agagaaacga agccaaatgg cttaaaaaga ataccgaaca actgaaatgg
      301 gcatgggaat atattgatgc acgtatctca cccacatatc tttcctggtt taatcctgta
      361 aatgataacg aaagatatat tgccattgta gttttactaa aaatgttatt ccccacagac
      421 acgccctgtt tactgacgcc aaaagaattt cacctaggat tcgctgcaaa agaaagattt
      481 ttcgacaaga tgcacaatgc attcagaaaa cagtttatcg acggaaaaaa agataaacgg
      541 gtacaaatca acgtaaagat atcgccatcc gcaaaaagcg ctctggacag actgaccaga
      601 gagcggaaaa caacacagca ggctattctg gaacaactga ttctgtacgg tagactggat
      661 taactttcac cactttcgct cttggcgatt ttcttctttt tgtagtgtct gcttgctgcg
      721 ggcatcaggt atttttccgt cattttgttg cagaacgctt caatgtagtt aaccattttc
      781 tggtgtgctt tcaccttttc ctccccaggg gacattttca gataaatgtc ctccttttcc
      841 acaagtgcat ttataaactc ctctaaacat ccctggttat aaagcgacgt gaaaaacaac
      901 acattcccca gtctctccag taattgcgat ttaaccctgt attgttgttt tctgaggatt
      961 gattgttttc taaagctcag tcttgttaaa agttgttcaa atcttggttc aaatttaaaa
     1021 acagatttta atcttaggga ttgatactcc tgataaaaca gttcaaacgt cggggatgct
     1081 gaccgaagaa caggtcgggc cacagcccgt ggactaaatc ccatgactga gatttccttt
     1141 ccctcctcct gcaagttcag aggcagttca gcaatccatg cgtccagctt agctgaatcg
     1201 ctctccttgc tcaaaacctc atacaaactg cccgtgaacg gttgagccga ttttggggtt
     1261 gcttcacctt ttgactgtct gaatccatcc acttttctcc ggatactacg cggaatcagc
     1321 ataaaaggcg gggcattaag agataaatgt ttaccagtca tacgatgaca ttgccctacc
     1381 tcgatccaac aggaaaattt actaaccgac cttatcttaa gcgcaattat gttaagcagg
     1441 tacagataac gcaccgtata acgattaaaa tgtttctcag gatcaaacga ttctgaccgt
     1501 tcccctctgt acacaagtcc actacgcttc agtattcgct gggtatcttt ccaggcctga
     1561 cttttacaaa cgctcattcg gcgtagcgta ttaatcgaca gtgatgccat cactcctccc
     1621 gaactttgtc actccagatt ttttcacgcc catattacct gaatgataaa ccggattagt
     1681 gtgatccggt tcagtgaaat caacaggata ccggtatgtc attcagccat tcttccctct
     1741 ctgcacaagt gaaatcatat ctgacgtttc ttcctgaaga aattcgccag aaaatccttg
     1801 aacatctcca cggtgttatt cattacgagc ccgtgattgg cattatgggt aaatccggca
     1861 ccggcaaaag cagcctgtgt aatgccattt ttcagtcccg tatctgcgcc acgcatcccc
     1921 tgaacggctg cacccgccag gctcatcgcc ttacccttca gttaggtgaa cgcagaatga
     1981 cgctggtcga tctgcccggc attggtgaga cgccacagca tgatcaggaa taccggacac
     2041 tttatcgtca attactgccg gaactggatc tgattatctg gatcctgcgg gctgatgaac
     2101 gtgcgtatgc tgccgatatt gccatgcatc agttcttact gaatgaaggc gcagatccct
     2161 cgcgttttct gtttgttctc agtcatgccg atcgcgtgtt tcctgccggc gagtggaacg
     2221 acacagaaaa atgcccgtcc cgtcagcagg aactctcact ggcaacagta acagcccggg
     2281 tggcaaccct gttcccttca tcatttccgg tactctccgt tgccgcacct gtcggctgga
     2341 accttccggc gtttgtgtcg ctgatgatcc acgcactgcc accacaggca accagcgcgg
     2401 tgtattcaca tatccggggg gaaaaccgct ctgaacaggc ccagaaacac gcacaacaga
     2461 cttttggtga aaccatcggg aaaagttttg acgacgccgt tgcccgtttc agttttccgg
     2521 cctggatgtt acagcttctg cgcaaaaccc gggaccgcat tatccacctg ctggtcacac
     2581 tgtgggatca cctgttctga gcgccccgga gtcccccgtg gcaaccgtat acctttacac
     2641 gacaacggat tacggtaacc actgccgtat accgtctgtc attatcaata acacgctctt
     2701 taatttcaga ggttagtcat gaataccaga attctttttc ccctgctgtt cactgtggca
     2761 tcattctccg cctccgccgg caactgggct gtcaaaaacg gctggtgtca gaccatgacg
     2821 gaagatggtc aggcgctggt aatgctgaaa aatggcacga ttggtattac cggcctgatg
     2881 cagggatgcc cgaatggtgt acagacgctc ctgggcagcc gtatcagtat taacggtaac
     2941 ctgatcccca catcacaaat gtgtaatcag cagacgggat tcagggctgt tgaggtggaa
     3001 gccggacagg cgccggaaat ggtcaaaaaa gccgttcact ccatagcaga gcgtgatgtg
     3061 tccgttttac aggcatttgg tgtacgaatg gaattcaccc gcggtgatat gctgaaggtc
     3121 tgcccgaaat ttgtcacgtc acttgctggt ttttccccga aacagacacc cgccattaat
     3181 aaagactccg tactgcaggc tgcccgacag gcatacgccc ggaaatacga cgaggaaaca
     3241 acagaaaccg ctgattttgg ctcttacgaa gtaaaaggca ataaggttga gtttgaagtg
     3301 ttcaatcctg aagaccgtgc gtacgacaaa gtgaccgtca cggttggtgc tgacggtaat
     3361 gccaccggtg ccagcgttga atttatcgga aaatagccgg tatgtcggac tgtcaccctg
     3421 ttttattgcc cgaaggccct ttctcacgcg aacaggcgat ggctgtcaca acagcttacc
     3481 gcaatgtgct tattgaagat gaccagggaa cgcatttccg gctggttatt cgcaatgctg
     3541 aagggcagct acgctggcgg tgctggaatt ttgaacctga tgccggaaaa cagctaaatc
     3601 cgtatctcgc cagtgaggga attctcaggc aataaacgtc attcatttca cccatcaggc
     3661 cgcgtcttct ccgggaggcg cggctttttc atttatatcg ctaactcatt cataaggagc
     3721 aaatcatgcg attagcaagt cgttttggtt atgcgaacca gatacgccgt gaccgcccct
     3781 taactcgcga agaactgatg caggtcgtcc ccagcgtatt cggagaagat aagcacacat
     3841 ccagaagcga gaactatacc tggatcccca ccatcacagt gctggaaagc ctgcagcggg
     3901 aaggctttca gccattcttc gcctgccaga cccgcgttcg tgacccgggc cgccggggat
     3961 acaccaaaca catgctgcgt ctgcggcggg ccggacagat aaccggtcag catgtccctg
     4021 aaattattct gctcaactcc catgacggtt catccagcta ccagatgctg ccgggttact
     4081 tcaggttcgt ctgccagaac ggatgcgtct gtggtcagtc tctgggagaa gtgcgtgttc
     4141 cgcaccgggg aaatgtggtg gacagagtca ttgaaggggc ttacgaggtg gtgggcgtgt
     4201 ttgaccggat agaggagaag cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac
     4261 gccaggcgct ggcacaggcg gcactgattt accgttatgg tgacgaacat cagcccgtca
     4321 ccactgctga cattctgaca ccacgacgca gggaggatta cggtaaggac ctgtggagcg
     4381 catatcagac catccaggag aatatgctga aaggcggaat ctccggtcgc agtgccagag
     4441 gaaaacgtat ccatacccgg gccattcaca gcatcgatac cgacattaag ctcaaccggg
     4501 cgttgtgggt gatggcagaa acgctgctgg agagcatgcg ctgatgcctt ttccctgaaa
     4561 gcgcagtcct gactcacttc cgtccctgcc tccggacatt cttccattca tttacttttt
     4621 ataaggaata atctcatgac aaccgttacg cataattcca ccacatcttc tgtttccgta
     4681 accgctgcat cagggaataa cccacctcag ttggttgcta ctctcgtccc tgatgaacag
     4741 cgcatcagct tctggtcgca acattttggc ctcattccac agtgggtgac cctggagccc
     4801 cgtgtcttcg gctggatgga ccgtctgtgc gaagactact gcggtggtat ctggaatctg
     4861 tacaccctga acaacggcgg ggcatttatg gcacccgaac cggatgacga tgatgacaaa
     4921 acatgggtac tgttcaatgc catgaacggc aaccgcgctg aaatgagccc ggaagccgcc
     4981 ggcattgctg cctgtctgat gacgtacagt catcacgcct gccgtacgga gtgttacgcc
     5041 atgacggttc attattaccg gctgcgagat tacgccctgc agcatccgga atgcagcgcc
     5101 attatgcgca tcatcgactg aatggaggca gggacaatgc agcagctttc ctttctgccc
     5161 ggagagatga cgcccggtga gcgcagcctc attcagcggg ccctgaaaac cctggaccgt
     5221 catcttcatg aacccggcgt ggccttcacc tccacccgtg cggcacggga atggctgatt
     5281 ctgaacatgg cgggactgga gcgtgaagag ttccgggtgc tgtatctgaa caaccagaat
     5341 cagctgattg ccagtgaaac cctctttacc ggcaccatca accgcacgga agtccatccc
     5401 cgggaagtga ttaaacgtgc cctgtaccac aatgccgctg ccgtggtgct ggcacacaat
     5461 cacccgtccg gcgaagtcac gcccagtaag gctgaccggc ttattacgga acgtctggta
     5521 cagacactgg ccctggtgga tatccgggtg ccggaccatc tgatagtcgg tggcagccag
     5581 gttttctcct ttgccgaaca cggtctgctt taacccgttg tcaccacatc acgcctgttt
     5641 tcacttttat cttctgtctt cagaggtatc ccactatgaa aattatcacc cgtggtgaag
     5701 ccatgcgtat tcaccaacaa catccggcat cccgtctttt tccgttctgt accggtaaat
     5761 accgctggca cggcagtgct gaggcgtata ccggccgtga agtgcaggat attcccggtg
     5821 tgctggctgt gtttgcagaa cgccgtaagg acagttttgg tccgtatgtc cggctgatga
     5881 gcgtcaccct gaactgagtc tggaagggca ttcagaagag cagaattatc gccaccaccg
     5941 gaccattctt aaccaatttt ctgtgaggat tttatcgtgt cagacacatt ctccgggaca
     6001 acgcatcccg atgacaatca cgaccgcccc tggtgggggc tgccctgtac cgtgacacct
     6061 tgttttggcg cccgtctggt gcaggagggt aaccggttgc attaccttgc cgaccgcgcc
     6121 gatatcagag gccggttcag cgacgcggat gcgtaccatc tggaccaggc ctttccgctg
     6181 ctgatgaaac aactggagct catgctcacc agcggtgaac tgaatccccg ccatcagcat
     6241 accgtcaagc tgtatgcaaa agggctgacc tgcgaagccg acaccctcgg cagttgtggc
     6301 tacgtttata tggctgttta tccgacgccc gaaacgaaaa agtaactctc cagaataacc
     6361 ttctgctccg gcctggtgtt ttcaccacgc cacttttcca tttttcatct ctgcatatca
     6421 ggaaaatctt cagtatgaaa acattacccg acacgcatgt ccgggaggca tcgcgctgcc
     6481 cgtctcccgt caccatctgg cagacactgc tgtcccggtt gctggacaag cattacggcc
     6541 ttacgctgaa tgacacaccg ttcgccgatg aacgtgtgat tgagcagcat attgaagctg
     6601 gcatttcact gtgtgatgcg gtgaattttc tcgtggaaaa atacgcgctg gtgcgtaccg
     6661 accagccggg attcagcacc tgtccccgct ctcagttaat aaacagtatt gatatcctcc
     6721 gggcccgccg ggcgacaggc ctgatgaccc gcgacaatta cagaacggta aatgacatta
     6781 cccaggggaa gcatccggag gcgaaacaat gaaactggcc ctgacgctgg aggcagacag
     6841 cgttaacgtg caggcgctga acatggggcg cattgtcgtt gacgtcgatg gtattaagct
     6901 cgctgaactg attaacaaag tcgctgaaaa cggttattca ctccgcgtgg tggatgaacg
     6961 tgacgctacc gaaacaccgg caacttctgc cagccctcat cagttgccgg aagacaatac
     7021 agtgatacag agcgtgatgc cattaaccac tgaaacagcg ctggatatcc tcattgcgtg
     7081 gctgcaggac aatattgact gcgaatcgga aattatcttt gacaacgatg aggataaaac
     7141 ggattcggca gcactgttgc cctgtattga acaggccagg gaggatatcc gtaccctgcg
     7201 ccaactgcag cttctgcaac agaaccggtg agtcttactc accagacttc attccactga
     7261 cgccagcctg aacagggctg gcgttttcat ttatctgcaa aaaggaatat cgattatgtc
     7321 tgaaatcaca gccccccgtc cggaagtggt caacgggcat acggacgtta tctgctcaac
     7381 cagtatccgc cacattctgg ctgtacgaaa gagtacgctg ctgcaaatcg acacacttac
     7441 ccggcaactg gcagaaatct cagcaatgac agaaagtatt ggcggtaaaa ccgtactgga
     7501 ctgggcgatg aaacaggatt ttcgctgcgg ttgctggctg atggaaaaac ctgaaacggc
     7561 aatgaaagct atcacccgca atctcgatcg cgaaatctgg cgtgacctga tgcaacgttc
     7621 cgggatgctg tcattaatgg atgcgcaggc ccgtgagaca tggtaccggt cactggaata
     7681 cgataatttt ccggaaatca gcgaagcgaa cattctgagc acatttaaac aactgcatca
     7741 gaacaaagat gaagtgtttg agcgaggggt gatcaacgtc ttcaggggac tgagctggaa
     7801 ttacaaaact aattgcccct gtaaatttgg cagtaaaatt atcattaaca acctggtaag
     7861 gtgggatcgg tggggatttc atcttatcac cgggcaacag gctgaccgac tcgccgatct
     7921 ggaaagaatg ttgcacctgt tcagcggtaa accgatcccc gacaaccggg aaaacatcac
     7981 cattcatctg gatgatcaca tccggtctgt tcagggtaaa gagtgctatg aagatgagat
     8041 gttcagcatc agatacttta agaaaggctc cgcgcacatc acgttcagga aaccagaact
     8101 ggttgacagg ctgaatgata ttattgcgaa acactatccg ggagtgttac cttcataata
     8161 aagctctaag aattgattcg attgtgtatt ttagcttcat tcccttacgc aaagtcattt
     8221 gggggttctt atgggggtta ctcatttata caacacagat taatcattta aaatcaaaac
     8281 attaacactc atattcgact cctgtgatcg ccaaaatgcc tcttcagtga tccccatcct
     8341 gcttatttct gagcaacctg accctgctga tgtatctggt agtatgacag agtgattcgt
     8401 ttttcttgcc gagtgtacga aaacgtcttc cttgccaccg actatcaacc gtcactcaaa
     8461 aaaacctctc tttttttcaa gctatcaaga ataagttgca ttttatctgc accggacacc
     8521 agccatccat atatggcttt tacctgctac aaagatgaag aagtgcataa atgtgatgtt
     8581 aaaacaatca aagaaaaatt acatgtgcaa taatttcact ataacattat aatccatggt
     8641 gaaaataaca ctgcacaagg attgtcatca tcaaactcat cggaactatt ttttctgtcg
     8701 tcattggtgt aatactgatt gtaagtatcc tttcaggaga ttatttttgc cgtgtggggc
     8761 atggaacttg taaagcagcc aaaagcgcta ggcatttaat tgaaagtaaa gagaaaaaca
     8821 gaagtagtcg cgtcaaagtc tttagcacgc atattaattt gagtcatcca gcggcatcct
     8881 ggct
//
LOCUS       DAZKHK010000171         8859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKHK010000171 DAZKHK010000000
VERSION     DAZKHK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.172"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..549
                     /locus_tag="ACHOS5_004509"
     CDS             <1..549
                     /locus_tag="ACHOS5_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9779222.1"
                     /translation="GGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSVSASFQ
                     RPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKDQYISGNNGGYLRNELSW
                     SLFSLPYVGTVRAVAALDGGWLHSDSDDPYSSGTLWGAAAGLSTTSGHVSGSFTAGLP
                     LVYPDWLAPDHLTVYWRVAVAF"
     gene            562..>8859
                     /locus_tag="ACHOS5_004510"
     CDS             562..>8859
                     /locus_tag="ACHOS5_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFP9779223.1"
                     /translation="MHQPPVRFTYRLLSYLVSAIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGKRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GKPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTA
                     GANRVTADGRVRALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARD
                     GDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRSDIVLSNGTLNS
                     DKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTVASDTLTTQG
                     QITAGQNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGASLTLGSTTLSNSGSL
                     LSGGPLTVNTRDFTQSGRTGAKGKVDIMASGKLTSTGLLVSDDALVLKAQDVTQNGVL
                     SGGKGLTVSAQTLSSGKKSVTHSDAAMTLNVTTVALDGETSAGDTLRVQADKLSTAAG
                     AQLQSGKNLSINARDARLAGTQAAQQTMVVNASEKLTHSGKSSAPSLSLSAPELTSSG
                     VLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSG
                     LITGDNGLMLNAVSLSNPGKIIADTLSVRATTLDGDGLLQGAGALALAGDTLSQGSHG
                     RWLTADDLSLRGKTLNTAGTTQGQNITVQADNWANSGSVLATGNLTASATGQLTSTGD
                     IMSQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNRGTVQGNHVTIRQNSVTNSGTLT
                     GIAALTLAARMASPQPALMNNGGSLLTSGDLTITAGSITSSGHWQGKRVLITADSLAN
                     SGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANSLT
                     SAGDITGVDTLTLTVNQTLNNQANGKLLSAGVLTLKADSVTNDGQLQGNATTITAGQL
                     TNGGHLQGETLTLTASGGVNNRSGGVLMSRNALNVSTATLSNQSTIQGGGGVSLNATD
                     RLQNDGKILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLATGSADVKGT
                     TLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQNGTGRLYSAGNLLLDAQ
                     DFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLGAG
                     EAFTNNGTLTAGKGNSVFSAQRLFLNAPGSLQGGGDVSLNSRSDITISGFTGTAGSLT
                     MNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQKDASGGANTEIINNSG
                     NIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSYGY
                     FTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPAGR
                     IASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKTFY
                     GSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTSSI
                     NNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELAGA
                     LKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDSHL
                     FAGLYELLGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFDTR
                     YVSNAVLSRTGSRYLNGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLDGS
                     ILWWESVTINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGGSINAQND
                     LSLDSSGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNITRR
                     QQWNAGSDSQYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGDLGMSA
                     GNDINIAANLINGSKSQSGFWHTDDNSASSTTSQGSSISAGGNLAMAAGHNLDVTASS
                     VSAGHSALLSAGNDLSLNAVRESKNSRNGRSESHESHAVVSTVTAGDNFLLVAGRDIA
                     SQAAGMAAENNVVIRGGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGGDT
                     TVNAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTR
                     TISEDSATREAGSLLSGNRVTVNAGDNLTVEGSDVVADRDVSLAAGNHVDVLAATSTD
                     TSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAG
                     KQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAIN
                     NAVTSAQETKESSDSRLKALQATKTALSGVQAGQAATMASATGDPNATGVSLSLTTQK
                     SKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAAN
                     DILLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFAGVNAAKGSEKGNGTEWT
                     ETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAA
                     GGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIA
                     STATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMI"
BASE COUNT         2153 a         2369 c         2638 g         1699 t
ORIGIN      
        1 gggggggtcg gaacactgaa tccggtattc acacggggga tgccctggtt cggcgcagaa
       61 agcgaccacg ggaaaagggg agacctgccc gtaaatcagt tccggaaatg gtcggtgagt
      121 gccagttttc agcgccccgt cacggacagg gtgtggtggc tgaccagcgc ttatgcccag
      181 tggtcaccgg accgtcttca tggtgtggaa caactgagcc tcgggggtga gagttcagtg
      241 cgtggcttta aggatcagta tatctccggt aataacggcg gttatctgcg gaatgagctg
      301 tcctggtctc tgttctccct gccatatgtg ggaactgtcc gtgcagtggc tgcactggac
      361 ggcggctggc tgcactctga cagcgatgac ccgtactcgt ccggcacgct gtggggtgct
      421 gctgccgggc tcagcaccac cagtggccat gtttccggtt cgttcactgc cggactgcct
      481 ctggtttacc cggactggct tgcccctgac catctcacgg tttactggcg cgttgccgtc
      541 gcgttttaag ggattattac catgcatcag cctcccgttc gcttcactta ccgcctgctg
      601 agttacctgg tcagtgcgat tatcgccggg cagccgttgt taccggctgt gggggccgtc
      661 atcaccccac aaaacggggc cggaatggat aaagcggcaa atggtgtgcc ggtcgtgaac
      721 attgccacgc cgaacggggc cgggatttcg cataaccggt ttacggatta caacgtcggg
      781 aaggaagggc tgattctcaa taatgccacc ggtaagctta atccgacgca gcttggtgga
      841 ctgatacaga ataacccgaa cctgaaagcg ggcggggaag cgaagggtat catcaacgaa
      901 gtgaccggcg gtaagcgttc actgctgcag ggctatacgg aagtggccgg caaagcggcg
      961 aatgtgatgg ttgccaaccc gtatggtatc acctgtgacg gctgtggctt tatcaacacg
     1021 ccgcacgcga cgctcaccac gggcaaacct gtgatgaatg ccgacggcag cctgcaggcg
     1081 ctggaggtga ctgaaggcag tatcaccatc aatggcgcgg gcctggacgg cacccggagc
     1141 gatgccgtat ccattattgc ccgtgcaacg gaagtgaatg ccgcgcttca tgcgaaggat
     1201 ttaactgtca ctgcaggcgc taaccgtgta actgcagatg gtcgtgtcag agccctgaag
     1261 ggcgaaggtg atgtgccgaa agttgccgtt gataccggcg cgctcggtgg aatgtacgcc
     1321 aggcgtattc atctgacctc cactgaaagt ggtgtcgggg ttaatcttgg taacctttat
     1381 gcccgcgatg gcgatatcac cctggatgcc agcggcagac tgactgtcaa caacagtctc
     1441 gccacggggg ccgtcactgc aaaaggtcag ggcgtcacct taaccggcga ccataaagcg
     1501 ggaggtaacc tgagcgtcag cagccggagc gatatcgttc tcagcaatgg aacgcttaac
     1561 agcgacaagg acctcagcct gaccgccggc ggcagaatca ctcaacagaa tgaaaaactg
     1621 actgccggcc gggatgtaac gcttgccgcg aaaaacatca cacaggatac cgccagccag
     1681 attaacgcgg cccgcgatat cgtgactgtc gccagtgaca cgctgacaac acagggacag
     1741 ataaccgccg ggcagaatct cacggccagc gccaccacgc tgacacagga cggaatattg
     1801 ctggcgaaag gtcatgcggg actcgatgcc ggcacgctga ataacagtgg tgctgttcag
     1861 ggggcttccc tgacgctcgg cagtacaacg ctcagcaaca gtggctccct gctcagtggc
     1921 ggtcccctga ccgtgaatac ccgcgacttt acccagagcg gccgcactgg cgcgaagggc
     1981 aaagtggata tcatggccag tgggaaactg accagtacag gtttgctggt gagcgatgat
     2041 gcactggtgc tgaaggcgca ggatgtgaca cagaacggtg tgctgtccgg cggcaaaggg
     2101 ctgacggtca gtgcgcagac gctgagctcc ggtaaaaaat cggtcaccca cagcgatgct
     2161 gcgatgacgc tgaatgtgac aacagtggcg ctggacgggg aaaccagtgc cggtgacacc
     2221 ctccgggttc aggcagacaa actgagtacc gcagcgggcg cacaacttca gagcggcaaa
     2281 aatctcagca tcaacgccag agatgcacgt cttgcaggta cgcaggcagc acaacagacc
     2341 atggtggtga acgccagtga aaagctcacc cacagcggga aaagcagtgc cccgtcgctc
     2401 agcctcagtg cgccggaact gaccagcagc ggcgtacttg ttggttccgc cctgaataca
     2461 cagtcacaga ccctgaccaa cagcggtctg ttgcaggggg aggcctcact caccgttaac
     2521 acacagaggc ttgataatca gcagaacggc acgctgtaca gtgctgcaga cctgacgctg
     2581 gatataccgg acatccgcaa cagcgggctt atcaccggtg ataatggttt aatgttaaat
     2641 gctgtctccc tcagcaatcc gggaaaaatc atcgctgaca cgctgagcgt cagggcgacc
     2701 acgctggatg gtgacggcct gttgcagggc gccggtgcac tggcgcttgc tggcgacacc
     2761 ctctcacagg gtagtcacgg acgctggctg acggcggacg acctctccct ccggggcaaa
     2821 acactgaata ccgcagggac cacgcaggga cagaatatca ccgtgcaggc ggacaactgg
     2881 gcgaacagtg gttccgtgct ggcaaccggt aaccttactg cttcggcaac cggtcagttg
     2941 accagtaccg gcgatatcat gagccagggt gacaccacgc tgaacgcagc caccacggac
     3001 aaccggggca gtctgctttc ggccggcacg ctctcccttg atggaaattc actggataac
     3061 cgcggcactg tccagggtaa ccatgtcacg attcgccaga acagtgtcac caacagtggc
     3121 acgctcaccg ggatcgccgc actgacgctt gccgcccgta tggcatcccc tcaacctgcg
     3181 ctgatgaata acggaggttc attgctgacc agcggcgatc tgacaatcac cgcaggcagt
     3241 attaccagtt ccggacactg gcagggcaaa cgggtgctga tcaccgcaga cagtctggca
     3301 aacagcgggg cgatccaggc ggctgacagc ctgactgcac gtctgacggg tgagctcgtc
     3361 agcacagcgg gcagcaaagt cacctcgaac ggtgaaatgg cgctcagtgc actgaattta
     3421 agcaacagcg gacaatggat tgcaaaaaat ctgaccctga aggcgaactc actgaccagt
     3481 gcgggtgaca tcaccggtgt ggatactctc acgctcacgg tgaatcagac gctgaacaat
     3541 caggcgaacg gaaaactgct cagtgcaggt gtgctgacgc tgaaggcgga cagtgtcaca
     3601 aacgacgggc aattacaggg aaatgccacc accatcacgg caggacaact cacaaacggc
     3661 gggcatctgc agggcgaaac gctgacgctg acagcctccg gtggcgtgaa caaccgttcc
     3721 ggtggtgttc tgatgagccg gaatgcactg aatgtcagta ctgcgaccct gagtaaccag
     3781 agcacgatac agggtggagg cggggtttcc ctgaacgcca cagaccgtct gcagaacgac
     3841 ggcaaaatcc tctccggcag taacctcacg ctgacggcgc aggtgctggc gaacaccggc
     3901 agcggactgg tacaggctgc caccctgctg ctggatgtgg tgaatactgt caacggcgga
     3961 cgcgtacttg ccaccggcag tgccgacgtt aaaggaacca cgctgaataa taccggtacg
     4021 cttcagggtg cggacctgct ggtgaattac cacacattca gcaacagcgg taccctgctg
     4081 ggaacctccg ggcttggcgt caagggcagt tcactgctgc aaaatggtac agggcggctg
     4141 tacagtgcag gcaacctgct gcttgacgct caggacttca gtggtcaggg tcaggtggtg
     4201 gccaccggtg atgtcacact gaaactgatt gctgccctca cgaatcatgg taccctggcc
     4261 gcagggaaaa ccctttccgt cacgtcgcaa aatgccatca ccaacggcgg tgtcatgcag
     4321 ggtgatgcca tggtgctcgg tgccggagag gcattcacca acaatggaac gctgactgcc
     4381 ggtaaaggca acagtgtttt cagcgcacag cgtcttttcc ttaacgcacc gggttcactt
     4441 cagggcggtg gcgatgtgag tctgaacagc cggagtgata tcaccatcag tggttttacc
     4501 ggcacggcag gcagtctgac aatgaatgtg gccggtaccc tgctgaacag tgcgctgatt
     4561 tatgcgggga ataacctgaa gctgtttaca gaccgtctgc ataaccagca tggtgatatc
     4621 ctggccggca acagtctgtg ggtacagaag gatgcttccg gcggtgcaaa cacagagatt
     4681 atcaataatt ccgggaatat tgagacgcat cagggcgata ttgttgtaag aaccgggcat
     4741 cttctgaacc agcgggaggg attttctgcc acaacaacaa cccggactaa cccctcatcc
     4801 attcagggaa tgggaaatgc tctggttgat attccccttt cccttcttcc tgacggcagc
     4861 tatggctatt tcacccgtga agttgaaaat cagcacggta cgccctgcaa cgggcacggg
     4921 gcatgcaata tcacaatgga tacgctttat tattacgcgc cgtttgctga cagtgccaca
     4981 cagcgctttc tcagcagcca gaacatcaca acagtaaccg gtgctgataa tccggcaggc
     5041 cgcattgcgt cagggcgtaa tctttctgct gaggctgaac gactggaaaa ccgggcgtca
     5101 tttatcctgg cgaatgggga tatcgcactc tcgggcagag agttaagcaa tcagagctgg
     5161 cagacgggga cagagaatga atatctggta taccgctacg acccgaaaac gttttacggt
     5221 agctatgcaa caggctctct ggataaactg cccctgctgt caccggaatt tgaaaacaat
     5281 accatcagat tttcactgga tggccgggaa aaagattaca cgcccggtaa gacgtattat
     5341 tccgttattc aggcgggcgg ggatgttaag acccgtttta ccagcagtat caataacgga
     5401 acaaccactg cacatgcagg tagtgtcagt ccggtggtct ctgcacctgt actgaatacg
     5461 ttaagtcagc agaccggcgg agacagtctg acacagacag cgctgcagca gtatgagccg
     5521 gtggtggttg gctctccgca atggcacgat gaactggcag gtgccctgaa aaatattgcc
     5581 ggaggttcgc cactgaccgg tcagaccggt atcagtgatg actggccact gccttccggc
     5641 aacaatggat acctggttcc gtccacggac ccggacagtc cgtatctgat tacggtgaac
     5701 ccgaaactgg atggtctcgg acaggtggac agccatttgt ttgccggact gtatgagctt
     5761 cttggagcga aaccgggtca ggcgccacgt gaaacggctc cgtcgtatac cgatgaaaaa
     5821 cagtttctgg gctcatcgta ttttcttgac cgcctcgggc tgaaaccgga aaaagattat
     5881 cgtttcctgg gggatgcggt ctttgatacc cggtatgtca gtaacgcggt gctgagccgg
     5941 acgggttcac gttatctcaa cggactgggt tcagacacgg aacagatgcg gtatctgatg
     6001 gataacgcgg ccagacaaca gaaaggactg ggattagagt ttggtgtggc gctgacagct
     6061 gaacagattg ctcagcttga cggcagcata ctgtggtggg agtcagtcac catcaacgga
     6121 caaacagtca tggtcccgaa actgtatctg tcgccggaag atatcaccct gcataacggc
     6181 agcgttatca gcgggaacaa cgtgcaactt gcgggcggca atatcaccaa cagcggcggc
     6241 agcatcaacg cacagaacga cctctcgctc gacagttccg gctatatcga caacctgaat
     6301 gcggggctga taagcgcggg cggtagcctg gacctgagcg ccatcgggga tatcagcaat
     6361 atcagctcag tcatcagcgg taaaaccgta caactggaaa gcgtgagtgg caacatcagc
     6421 aatatcaccc ggcgtcagca atggaatgcg ggcagtgaca gccaatatgg tggtgtgcat
     6481 ctcagcggta cggacaccgg tccggttgcg accattaaag gcactgattc actttcgctg
     6541 gatgcaggga aaaacattga tattaccggg gcaacggtct cgtccggtgg agaccttgga
     6601 atgtctgcgg gtaatgatat caacattgcc gcaaacctga taaacgggag caaaagtcag
     6661 tccggtttct ggcacactga tgacaacagt gcatcatcca ccacctcaca gggcagcagc
     6721 atcagcgccg gcggtaacct ggcgatggct gcaggccata atctggatgt cacggcatcc
     6781 tctgtttctg ccgggcacag cgccctgctt tctgcaggta acgacctgag tctgaatgca
     6841 gtcagggaaa gcaaaaacag tcgcaacggc aggtcagaaa gtcatgaaag ccacgcagtt
     6901 gtgtccacgg tgacagcggg cgataacttc ctccttgttg ccggtcgtga tattgccagt
     6961 caggctgccg gtatggctgc ggaaaataac gtggtcatcc ggggcggacg tgatgtgaac
     7021 ctggtggcag agtctgccgg cgcaggcgac agctatacgt cgaagaaaaa gaaagagatt
     7081 aacgagacag tccgtcagca gggaacggaa atcgccagcg gtggtgacac caccgtcaac
     7141 gcaggacggg atatcaccgc tgttgcgtca tccgttaccg caaccggcaa tatcagcgtg
     7201 aatgccggtc gtgatgttgc cctgaccacg gcgacagaaa gtgactatca ctatctggaa
     7261 acgaagaaaa aaagcggagg ttttctcagt aagaaaacca cccgcaccat cagtgaggac
     7321 agtgccaccc gtgaagcagg ctccctgctg tcggggaacc gcgtgaccgt taacgccggt
     7381 gataacctga cggtagaggg ttcggatgtg gtggctgacc gggatgtgtc actggcggcg
     7441 ggtaaccatg ttgatgttct tgctgccacc agtacagata cgtcctggcg ctttaaggaa
     7501 acgaagaaat ccggtctgat gggtaccggc ggtattggtt tcaccattgg cagcagtaag
     7561 acaacgcacg accggcgcga ggccgggaca acgcagagtc agagtgccag caccatcggc
     7621 tccactgccg gtaatgtcag tattaccgcg ggcaaacagg ctcatatcag cggttcggat
     7681 gtgattgcga accgggatat cagcattacc ggtgacagtg tggtggttga cccggggcat
     7741 gaccgtcgta ctgtggacga aaaatttgag cagaagaaaa gcgggctgac ggttgccctt
     7801 tccggcacgg tgggcagtgc catcaataat gcggttacca gtgcacagga gacgaaggag
     7861 agcagtgaca gccgtctgaa agccctgcag gccacaaaga cagcgctgtc tggtgtgcag
     7921 gccggacagg ctgcgacaat ggcctccgca accggtgacc cgaatgcgac gggagtcagc
     7981 ctgtcgctca ccacccagaa atcgaaatca caacaacatt ctgaaagtga cacagtatcc
     8041 ggcagtacgc tgaatgccgg gaataatctg tctgttgtcg caaccggtaa aaacaggggc
     8101 gataaccgcg gagatattgt gattgccgga agccagctta aggccggtgg taacacaagc
     8161 ctggatgccg cgaatgatat tctgttgagt ggcgccgcaa acacacaaaa aacaacgggc
     8221 aggaacagca gcagtggcgg tggcgtgggt gtcagtatcg gtgccggtgg taacggtgcc
     8281 ggtatcagcg tctttgccgg cgttaatgcg gcaaaaggca gcgagaaagg taacggtact
     8341 gagtggactg aaaccacaac agacagcggt aaaaccgtca ccatcaacag tggtcgggat
     8401 acggtactga acggtgctca ggtcaacggc aacaggatta tcgccgatgt gggccacgac
     8461 ctgctgataa gcagccagca ggacaccagt aagtacgaca gtaaacagac cagcgtggct
     8521 gccggcggca gttttacctt tggctccatg accggctcag gttacatcgc tgcctcccgg
     8581 gataagatga agagccgctt tgactccgtt gctgaacaaa ccggaatgtt tgccggtgat
     8641 ggtggcttcg acattacagt gggcagacat acccaactgg atggtgcggt gattgcctcc
     8701 actgccacac cggataaaaa ccacctggat accggaacgc tggggttcag tgaccttcac
     8761 aacgaagcgg attataaagt cagtcacagt ggaatcagtc tgagcggtgg tggcagcttc
     8821 ggggataaat ttcagggtaa catgccgggt ggcatgata
//
LOCUS       DAZKHK010000172         8821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKHK010000172 DAZKHK010000000
VERSION     DAZKHK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.173"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..3351)
                     /gene="dgcE"
                     /locus_tag="ACHOS5_004511"
     CDS             complement(34..3351)
                     /gene="dgcE"
                     /locus_tag="ACHOS5_004511"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9779224.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDGNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVFHDFFQQAAKAIASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PAQLLHLIIPAEAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            3669..4310
                     /gene="udk"
                     /locus_tag="ACHOS5_004512"
     CDS             3669..4310
                     /gene="udk"
                     /locus_tag="ACHOS5_004512"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9779225.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            4402..4983
                     /gene="dcd"
                     /locus_tag="ACHOS5_004513"
     CDS             4402..4983
                     /gene="dcd"
                     /locus_tag="ACHOS5_004513"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP9779226.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            5005..6858
                     /gene="asmA"
                     /locus_tag="ACHOS5_004514"
     CDS             5005..6858
                     /gene="asmA"
                     /locus_tag="ACHOS5_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP9779227.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTLSLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKINGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(6926..8509)
                     /gene="yegH"
                     /locus_tag="ACHOS5_004515"
     CDS             complement(6926..8509)
                     /gene="yegH"
                     /locus_tag="ACHOS5_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9779228.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGNYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT         2230 a         2272 c         2263 g         2056 t
ORIGIN      
        1 tagcgtcgca tcaggcaatg ggcaaacgct cccttagtta atcgcgaaat aactgctatt
       61 taccagcaaa tccagcggtt gggcatcggc aatcacatca ccataaatca gatcgacgcc
      121 gatgcccgaa agcgtatcca tcactaaggg taaaacgact ggcccggcga tggtcttcat
      181 cccgagccgc tgagcgtgcc cctgaatgat cgtaatcagc atctcatcca tcaaattacc
      241 ctgcacgttg gcgcataact caccatcaag tagcaggtaa tctgccatat ttgctttcag
      301 cgagttgaag atttgcagat ctcggcccac ctgactgaat acgatccgac atcccgccag
      361 ccgcagtttt tgcacgcttt ctgcgtgatc gaaaatcgct tctgccggaa taatcagatg
      421 taataactgt gctggtagag ggctattttc caactgctca agcagttcat tcaccagcgt
      481 ggcgctactc aaaccagcaa cggaaagggg gagggcgatg cttaagcctt tactggcaat
      541 cgcttttgcg gcctgctgga aaaaatcgtg gaatacccgt tggtcaagag catggctaag
      601 tgctggatcg ctgaagctac gacgaaatgt ttgttcatca ataatctcgc tttcgcaact
      661 ccagagctta agtgaaatca gccacaaatt acttgcttcc gggagccgtg gcgaagcgat
      721 gccgtgggcg atcatcatca actgattctc tttaatcatc cgccactgtt catcaagcga
      781 tatcgctgcc cgttcgctat gtgcggcagc ttgctgcggt tcgtaaatcg tcacccggcc
      841 ccggccacca tttttggagg cataacaggc gatatcagcc tgcgacatca cttcagctgc
      901 ctgatgattg ttgccatcaa tcaaggtaat tccggcactg gcacctaccc gatgcacacg
      961 tccttcccat ataaagtgat agtcattcac ggcactgata atgcgtgtag cgataaaacg
     1021 agcgctttca acattgcagt ctggcagtag cagaccaaat tcatcaccac cgagccgcgc
     1081 cagcacgtcg ctggagcgca gcatactcag catcaacgac gccagttcgc gcagtaaagc
     1141 gtcgcccgcc gcatgcccgg cgctgtcatt caccgcttta aagcgatcaa gatcgataaa
     1201 caccagggca tgtcgctgat gcgtactgtt taccgtttgc agcaggatac gcagttgctt
     1261 ctcaaaactg gcgcgattgg cgagatgcgt cagtgcatca tgggaggcgc tgtagctcag
     1321 ttggcgcagc attttgcgtg attcggtaac gtcctgaatc accagtaccg agcctatatt
     1381 gctgccgtcc agagtactta acggcgtaat actgtaatgc acgtcgtaac tgccgccgct
     1441 ccggcagtgc aataccacat cttgttcaat cgcggaacgt gaggtgtcag cactgtaaat
     1501 gttctccatt aatggtccgt tgtcgccaaa agtaatatgc aacaccgtca ggagcggaac
     1561 acctaacgct tcttcctgcg tccagccgct catcttctcc gccactggat tcataaaggt
     1621 aattttcatt gccatatcaa tacagaccac ggcttcgccg atggagtcca gcgtaatgtg
     1681 caggcgctct ttttcctgaa acaatgcctc gttaagctgt ttaacctccg tcatatccat
     1741 attaatgccg agcaggcgtt caacttcgcc ttctttattc agtacccggt tggcgagagc
     1801 gcggatatgg cgaataccat ctttcacggc aatgcgaaat tccagtttaa agggcgagcg
     1861 tgattgcaac gaatcacgaa tcactttttc ggcgtgctgg cgatcttccg gcagcacgca
     1921 ctcgtaccac acctgccagt tcggtttgat atgcggagga atttcataca gctcgaacat
     1981 ccgcttatcc caactaaaaa tattcggctt cagctcccac tcccagatgc caatcccgcc
     2041 cgcttcgttg gccagcgtga tgcgctccat cagttgttga ttgacctgtt cggtgcgttt
     2101 tagctcgtta atgtcttcaa tctgagcgat aaaatagagc ggcgtgccat ccgtgtggcg
     2161 caccagtgag acggcaagca acgcccagac aacatcgcca ttgcggttgt agtaacgttt
     2221 ttccattgaa taggtgttta tttcaccgct tatcagcttt tcaacctgtt ggagatcttt
     2281 attgagatcc tccggccagg tcagttgctg aaaggtgagt ccgcgcagct cttcctgact
     2341 gtaaccgaga aactggcaga gcgctttgtt ggattgcagc cattgtccct cggtgcccac
     2401 taacgccatg ccgatagcgg aatattccat cgcgttccga aagcgggttt cgctttcgga
     2461 aatgtgtttg cgttccgcac gaaaggcata catcaccatc gtcatgatgt tggcgggcag
     2521 caggatcagc aaaaacggta gccacggcat atggctcatc aggtacgtac gcggcgtagc
     2581 aagcagggag ggatccgcgg ccatcatcag cgacaccatc atcaccgtgg taaggaagat
     2641 caaaaaggct tccattcgtg gcaggcgcac ggcgctccac atcaacagca caataatgaa
     2701 agtaaaaggc cacggcagat acagcatcga aagccagctt aacgtcagcg tgatggctaa
     2761 cgtgagcagc gactcaaaaa gcaaccttgg gttgcgatgg cgcagcagat agtgtggttt
     2821 aaataacaat cccagcggca ccagtgccag agcgccgatg gattctgaca gtacccatat
     2881 caaaaatgcc ctgagaggat cgtctccggg cgtcagcagg acaaccagaa caccccccaa
     2941 cagaggcgga ataatggcgc tgccgagtgc cagacgcagc cagtcagcca gattttgcaa
     3001 ggggttatac cacggcagca atttacgtag cagcactgcc ccgaccacgg cttcaacaat
     3061 attgatggtc gtccaggtca tgttcagcga gctggtggaa aaaagcagga tggatgcggc
     3121 gatatttccc agcgagcagc tcagcgcaat tcccggccac atgcgcccgg catggcgata
     3181 aaacgccacc atcatgatgg aggtcgggaa ccacagtggg gcgagttggg tgccaaactg
     3241 cgaaagctca agtgagaaaa gggtgaagat aaacgagact aaacctaaac tgaccaggtg
     3301 aagcagcggg tggggcaggg caattaatac atgctgtgat tgtttgctca ttaccgcttt
     3361 atccgcaagg ccaggtcgcc agtattcgtt tctgtgttta tgctagtaca aaagttttac
     3421 attttatatg cgaattgctc ataaagtgac gtaaaggcgg ataatttgcg caactgcgta
     3481 taacattttt taccttgtat aaaactgatc aacgtaattt gccaccggga attcttcgtc
     3541 aacaattaac tctgagcgcg cggcaacgct attcgactgg tatcaggcgg atgaaatccc
     3601 tataattgcc gcgtttggcg cttcgtcgcc cccttcctaa catccaggtt aatcaggtcg
     3661 ctaaatatat gactgatcag tctcatcagt gcgtcattat cggtatcgct ggcgcatcgg
     3721 cttccggcaa gagtcttatt gccagtaccc tttatcgtga attacgtgag caagtcggtg
     3781 atgaacacat cggcgtaatt cccgaagact gctattacaa agatcaaagc catctgtcga
     3841 tggaagaacg cgttaagacc aactacgacc atcccagcgc gatggatcac agcctgctgc
     3901 ttgagcattt acaagcgttg aaacgcggct cggcaattga cctgccggtt tacagctatg
     3961 ttgaacatac gcgtatgaaa gaaacggtga cggttgagcc gaagaaggtc atcattctcg
     4021 aaggcatttt gttgctgacg gatgcgcgtt tgcgtgacga acttaacttc tccattttcg
     4081 ttgatacccc gctggatatc tgcctgatgc gccgcatcaa gcgtgacgtt aacgagcgtg
     4141 ggcgttcaat ggattcagtg atggcgcaat atcaaaaaac cgtgcgcccg atgtttctgc
     4201 aattcattga gccttctaaa caatatgcgg acattatcgt gccgcgcggc gggaaaaacc
     4261 gcatcgcgat cgatatattg aaagcgaaaa taagtcagtt ctttgaataa gcttgataaa
     4321 ttgtgtaccg ttcagtgata acctggtatg cccttgacgt aattggcgtt aagggagtga
     4381 tgctcgaaag gagaaaatgc catgcgtctg tgtgaccgag atattgaagc ctggcttgat
     4441 gaaggccgtt tgtcgatcaa cccacgtccg ccagtggagc gtattaacgg cgcgacggtg
     4501 gatgtgcgcc tgggcaataa atttcgtacc ttccgtggtc acacggcagc gtttatcgat
     4561 ctgagcggtc ccaaagatga agtgagcgcc gcgcttgacc gcgtgatgag cgatgaaatc
     4621 gtgctcgacg agggcgaggc gttttatctt cacccaggag agctggcgct ggcggtgacg
     4681 ctggagtcgg tgacgctgcc agccgatctg gtgggctggc tggacgggcg ttcttcactg
     4741 gcgcgtctgg ggctgatggt gcacgtcacc gcgcaccgta tcgatccggg ctggtctggc
     4801 tgcattgtgc tggagtttta caactccggt aagctgccgc tggcgctgcg tccgggcatg
     4861 ttaattggtg cgctgagctt tgagccgctc tccggcccgg cggcgcgacc ttacaaccgc
     4921 cgtgaagatg cgaaatatcg caaccagcag ggcgcggtag ccagccgaat cgataaagac
     4981 taattcaggc ccattgagga taccatgaga cgatttctga cgacgctgat gatactcctg
     5041 gtcgtgctgg tggccgggtt atctgcgtta gtgttgctgg tgaatccgaa tgatttccgc
     5101 gactatatgg tcaagcaagt tgctgcacgt agcggttatc aattgcagct cgacgggcca
     5161 ctgcgttggc atgtctggcc gcagcttagt atcctctccg ggcgaatgtc tctcaccgcc
     5221 cagggcgcaa gccagccact ggttcgcgcc gacaacatgc gtctggacgt ggcgctttta
     5281 ccactactga gtcatcaact gagcgttaag caggtgatgc taaaaggggc agtgatccaa
     5341 ctgacgccgc agacggaagc agtgcgcagt gaagacgctc cggttgcacc gcgcgacaat
     5401 accttgccgg atctgtcaga cgatcgcgga tggtcgtttg atatatccag tcttaaggtg
     5461 gcggacagcg ttctggtgtt ccagcatgaa gatgacgagc aggtgacaat ccgcaatatc
     5521 cgcctgcaaa tggaacaaga tccccaacat cgtggctcat ttgagttttc cgggcgggtt
     5581 aatcgcgatc agcgcgatct cacattatcc cttaacggta cggtggatgc ttctgattat
     5641 ccgcatgatt taacggcggc tattgaacaa attaactggc agttgcaggg tgccgattta
     5701 ccaaaacaag gtattcaggg gcaggggagt ttccaggtcc agtggcagga gtcacataaa
     5761 cgcctttcat ttaaccaaat tagtttgacc gccaatgaca gtacgctgag cgggcaagca
     5821 caggtcacgt tgacagagaa accggaatgg cagctgagac tgcaattccc gcaactgaat
     5881 cttgacaacc tcatccccct taatgaaaca gcgaatggtg agaacggtgc cgcgcagcaa
     5941 gggcagagcc aatcaacgtt gccgcgcccg gtcatttctt cgcgtattga tgaaccggcc
     6001 tatcagggac tgcaaggctt tacggctgat attttgttgc aggccagtaa cgtgcgctgg
     6061 cgcggaatga attttacaga tgttgccacg caaatgacca acaagtcggg tttgctggaa
     6121 attactcaac tgcagggcaa aattaacggt ggacaggttt cactgccggg cacgttggac
     6181 gcgacatcta taaatccgcg gataaacttc cagccacggc tggaaaacgt tgagattggc
     6241 accattctga aggcgtttaa ctatccgatt tcgttgaccg gaaagatgtc actggctggt
     6301 gatttctccg gtgctgacat agatgccgac gcattccgcc acaactggca aggacaggca
     6361 catgtcgaaa tgaccgacac acgcatggaa gggatgaact tccagcagat gattcagcaa
     6421 gcggtagaac gtaatggtgg tgatgtgaag gccgctgaaa acttcgataa cgtgacgcgt
     6481 cttgaccgct ttaccaccga tttgacgttg aaggatggcg tcgtgacgtt aaacgacatg
     6541 caaggtcaat cgccagtgct ggcgctgaca ggggaaggca tgttgaatct ggcagatcaa
     6601 acctgtgaca cccagtttga tattcgagtc gtgggtggct ggaacgggga aagcaaactg
     6661 attgatttcc tcaaagaaac gccagtaccg ctgcgggttt atggcaactg gcagcaactt
     6721 aattacagcc tgcaagtgga tcaattactg cgtaaacatc tacaggatga agcaaaacgc
     6781 cgtcttaacg actgggccga gcggaataaa gattcccgca atggcaaaga tgtgaagaag
     6841 ttgctggaga agatgtaggt taccaggtga cctgtcctgt aggcctgata agacgcgtaa
     6901 agcgtcgcat caggcctaca tgccatcaca cctcgtactc catctcgcca tccttacgca
     6961 gcggaataat ctgtaccttc tgcacgcgat ggctttccac ctgcaacgtt ttaagcaaat
     7021 aattccccac ctgaacttct tcgccaggtt ttggaatacg ttgcaaatat tccatcaata
     7081 gcccagcaat ggtgtgatat tcacgttttt catccagcgg caacggcaca tattgcacca
     7141 gatcttccag cggcatatga ccattcgccg tccaggaacc gtccgcattc ttctgaatat
     7201 catggcgggc gtcgatctct tccacttcgt tcggtaagtt accggcaatg gtttcagtga
     7261 cgtcacttaa tgtcacaatc ccttccaccg agccaaactc atccaccaca aaagcaaagt
     7321 gtgtgcgggc attacggaac tgctccaggg caggtaacaa cggcaaggtt tccgggaaca
     7381 ccagcggctg acgaatcaac acccgcaggt tgagcggttc gccgcgcagt gactgttgta
     7441 acaggtcgat aacgtgaaca acaccgagca aatcttctgc gtcatcgcca tcagtaacaa
     7501 caagccgcgt atgctgattt cgctccagta actggcggat ctcgtcttcc ggcgcgttga
     7561 gatcgatatg ctcaatatcg tggcgcgacg tcataatgct gctgacggta cgctggttaa
     7621 gattaagtac ccgttcaatc atccgccgtt cctgcggatt aaagatctgc tggttgccat
     7681 gatccaccag catggacgcg gtttcggcgt ccagttccgc atcttctttt tgcccgctaa
     7741 gcaggcgcat taccgcttcg gtagtccgct ggcgcagcgt ctgattagcg gaaagaaaac
     7801 gtcgccggtt aaagatagcc aactgattga gcgcctcgat catcaccgag aaaccaatgg
     7861 cagcgtacag gtagcctttc ggaatgacga agccgaaacc ttccgccacc aggctaaagc
     7921 caatcatcaa caggaagctt aagcagagaa taacgatcgt cgggtgactg ttaacgaact
     7981 gggttaacgg cttgctggcc atcaacatca ggctgatagc gataaccacg gcggccatca
     8041 tgaccagtaa atggtcgacc atcccgacgg cggtaatcac cgagtcgagt gagaagatgg
     8101 cgtccagtac cacaatttgt gtcaccaccc cccagaactt cgcacctttg cgttgggtgg
     8161 ggttattgct gtctttccct tccagtcgtt cgttcagctc catcgtggct ttgaacaaca
     8221 ggaagaaacc accaaacagc atgattaaat cgcgggcgct aaaggtaaac gagcggaagc
     8281 tgaacagcgg ttgggtcagg gtgaccagcc aggagattga cgccagcagt aacaggcgca
     8341 ttaacatcgc cagcaggagc ccggtaaccc gtgcgcggtc gcgctgctta ggcggtagtt
     8401 tttcggcgag gatggcaata aagaccaggt tatcaatgcc gaggaccagt tcgatcacaa
     8461 tcagcgtgat taacccagcc cagatagacg gatcggcaat ccattccata gtaagtacaa
     8521 aacctattcg ttgtatgaca aatgtcacaa ttcgatcatg gataaatcgg ggcaaaaatg
     8581 caatgttgca gcactattta tctcatatta tttgagggtt ataacctcac attatccccg
     8641 aattataagt ggtaataata aaacgtcatt taagaaatat cgcatgtgga gacgccatat
     8701 taaatattgt caacctaaag aaactcctaa aaaccatatt gaatgacact taatataatt
     8761 cttaaaaata gccaattgcc gaattgttac cttgcctgct attccgttag ctgtaacact
     8821 t
//
LOCUS       DAZKHK010000173         8577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKHK010000173 DAZKHK010000000
VERSION     DAZKHK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.174"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            321..1130
                     /gene="pduF"
                     /locus_tag="ACHOS5_004516"
     CDS             321..1130
                     /gene="pduF"
                     /locus_tag="ACHOS5_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFP9779229.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVTGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
     gene            1333..2247
                     /gene="pocR"
                     /locus_tag="ACHOS5_004517"
     CDS             1333..2247
                     /gene="pocR"
                     /locus_tag="ACHOS5_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HFP9779230.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSLRQTRAPGPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSDNAAMETV"
     regulatory      2339..2514
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2732..3169
                     /locus_tag="ACHOS5_004518"
     CDS             2732..3169
                     /locus_tag="ACHOS5_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP9779231.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PFDGGPVQSLSDAYSYYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLNGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            3303..3848
                     /gene="cobU"
                     /locus_tag="ACHOS5_004519"
     CDS             3303..3848
                     /gene="cobU"
                     /locus_tag="ACHOS5_004519"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP9779232.1"
                     /translation="MMIFVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3845..4588
                     /gene="cobS"
                     /locus_tag="ACHOS5_004520"
     CDS             3845..4588
                     /gene="cobS"
                     /locus_tag="ACHOS5_004520"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP9779233.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            4600..5679
                     /gene="cobT"
                     /locus_tag="ACHOS5_004521"
     CDS             4600..5679
                     /gene="cobT"
                     /locus_tag="ACHOS5_004521"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP9779234.1"
                     /translation="MQILADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMTGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            5741..6667
                     /gene="ldtA"
                     /locus_tag="ACHOS5_004522"
     CDS             5741..6667
                     /gene="ldtA"
                     /locus_tag="ACHOS5_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9779235.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPVSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIINQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVNINRNGF"
     gene            6841..7806
                     /locus_tag="ACHOS5_004523"
     CDS             6841..7806
                     /locus_tag="ACHOS5_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9779236.1"
                     /translation="MTKQNKNSRWQGYDPRYIYSLVVRRYFADMKTKIEIAEELGVSR
                     FKVARLIDEAIEQDYVRFIFPKQQAMDEEIANNLRKKFHLEDAIVLSVAESWTTQEEL
                     NHKLGEVTAEYLMQSLHEGMKVGIAWGRVLSSTVSKLSKLPPLDVVQLSGVHPGIEFS
                     QGPIDLIHKIAAISQGKAHPMYVPMWVDDEELAARLAGDPAVLDTQQYYSQLDVVITG
                     IGDWKSGSSSLCKIFPDTWCEALFQQDIAADVCISLVSREGKILHSPIERLGFGISTD
                     QLQKAKKVIGVAGGEEKYEGILASLKSGLLNVLITDFDTAIKLLD"
     gene            complement(7858..8316)
                     /locus_tag="ACHOS5_004524"
     CDS             complement(7858..8316)
                     /locus_tag="ACHOS5_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9779237.1"
                     /translation="MKFEILVNDVDNSISDYQTAITFAGTQLLEKGYITAEYIDACLE
                     REKSYPTGLMMANGQGIAIPHADYTLVNVNSISIVRFANDVTFGQMEDADLTVDCSIM
                     FNLAFATSDQHMSILRRLFTLFQDNAFVETCRNLPATRVAKYVEDTLAAS"
     gene            complement(8327..>8577)
                     /locus_tag="ACHOS5_004525"
     CDS             complement(8327..>8577)
                     /locus_tag="ACHOS5_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9779238.1"
                     /translation="ASSSIMVSVLQDFLKEEKIEAQVDKSSLMACTSDTFNSYDLVVS
                     STKLDNPAITTRVIVGAGLLTGLGEDEIFAAVKEEMLK"
BASE COUNT         2141 a         1783 c         2233 g         2420 t
ORIGIN      
        1 gagtttaatc gcgagtaata tttcgttgag tttggttcga aggaaggaac tgtgatattt
       61 gtttcaattt tattatgtca tgatggcaat tttttattgt aaaagtttgg tggaaattat
      121 tactttcaac actgattaac gctgtgttgc atgattcggc aatgacaata atttgttatg
      181 ttagcacgat ttttacacga gtctgtcatg catgttggca atattttctg cagagcaatt
      241 ttttatagtt gcgctcggta tacacaacaa tcagcgcata gttggctgat aaagaaaaac
      301 tccgtaaaga aggtgtcact atgaatgatt cactcaaggc gcaatgcgtt gccgagtttt
      361 tgggcaccgg actgttcctc ttttttggca ttggttgttt gtgcgccctg aaggttaccg
      421 gggcaagtct gggactgtgg gaaatttgta tcatttgggg attagggatt tcgcttgctg
      481 tttaccttac ggcaggtatt tccggcgctc atctcaaccc ggcagttacc attgctctgt
      541 ggctgtttgc ctctttccca gcccgtaaag tgctgccatt ttgtgtggcg caattagcgg
      601 gtgcctttgg tggtgcggtt ctggcatatt ctctctacag cagtttgttt accgattttg
      661 aatctgctca caatatagtg cgtggtagcg cagaaagttt acaactcgcc agtatcttca
      721 gtacttatcc agcagcggcg attaatgtat ggcaagcagc gttggtcgaa gtggtcataa
      781 cgtccatgtt aatggggttg attatggcgt tgaccgatga cggtaatggc gtacccaaag
      841 gaccgcttgc gcctttactc attggtattc tggttgctgt tattggtgct tctaccggac
      901 cattaaccgg ttttgccatg aatccagcgc gtgattttgg acctaaaatt ttcacctggt
      961 ttgcgggttg gggggaaatt gcgatgactg gtggacgcaa tattccttat ttcattgtac
     1021 ccattattgc accgatcatt ggggcatgtg ttggtgcggc gatttatcgc tatctcattg
     1081 caaagaattt acctgtcaat agtgtaacgc ctaaagaaat cagcgaataa cgaaataatt
     1141 tggcagttat aaacgtttat ttttttactg tttgtgatct gctttccgaa cttattaaaa
     1201 agtctcccgc ttcacggagg cttttgttat tgtcctttgt gcttctcgtc agtaaatcat
     1261 caaggatgat gtgaagatta aatgtaaaac aattgttttg ctataacaat aaattaactg
     1321 ggaggtttta tcatgatttc tgcggcgaca cttaattcag aactcattaa taaaatcgcg
     1381 caggattttg cgcaggcgac cagccttgcg gttgtcgtgg tcaatattca cggcgatgaa
     1441 atttccgagc tgtttaactt cactccattt tgtcagatga tgcggcaaca ccctcagctg
     1501 agcactcgat gtcgcatgag cgatcgttgt gggggtctgg aagcctctaa atccgatcag
     1561 ccttgtattt atcgctgtca tgccggatta accgatttct ctattccact ggtaatcgcc
     1621 gggcatttag ttggttttgt attgtgcggg caagttcgtc tgcgtaatga tgatgtcgat
     1681 ttggtcgata ttctgaatgt cgatgattgc tggcaggcag atcccgaatt acttaatgaa
     1741 tttcgcaaag tccctgagat ggattactca agagttatgg cctctgcgga tttgttgaag
     1801 ttgattgtag aaaattgcct aaaaaaacaa ctcaattttg tggttattaa agagaacaaa
     1861 tctcagtcag attcgttgcg ccagactcgt gcgcctggtc cccacgacaa taaaatgaaa
     1921 aaagctttac gttatattga tgcgcattta tcagacgatt tgcgtctgga agatgtcgcc
     1981 tcgcacgttt atctaagccc ttattatttc agtaagttat ttaagaaata ccatggaatc
     2041 gggtttaatg cgtgggtaaa ccaacaacgg atggccagcg ctcgtgagtt actgtgccat
     2101 agtgactgga gcatcgccag cattgcgcgt aatttaggtt tttcgcaaac aagttatttt
     2161 tgcaaagttt tccgtcagac ttatcaggtt acgccgcagg tgtatcgaca gcaagtgagt
     2221 gataatgcgg cgatggaaac ggtatagagt aaatgcccca aaaaaagagg acggtttaaa
     2281 taaactctgg aatgaataat taacgttatt tccctgttat gggaaaagtg aaatcagctc
     2341 ataacatacg ctgacaggtg tggcacattt gtgcctggga agagggtgaa aatccctcgc
     2401 agcccccgct gctgtaatgc tgacaaaccc gaaaatgcca ctgatcgcga gattgggaag
     2461 gtcgggagag gatgacgcta agccagaaga ccagccagtc agtcaatacc aacaacttcg
     2521 gtgggaagtg ggttgcccgg aagcgtccgg tagagtacag gcgcgtcttt atacccctac
     2581 caccctgaaa ggatcagggt atggcggcat catttagttc ttttatcgca gtgccgacag
     2641 gcaccagggc tgcacttaag gtgtggcaat cttgtttcat ggcttatcaa ccagaccgcc
     2701 attacaggaa attaagtgga gggcgagggg catgaaaacg ataaaacccg aatcgatagc
     2761 tcttttttgc ctcttatctg gagatgtgga tctggcgaaa catctggcgg aaatattgcc
     2821 aataacctgt tttgttggtg aggactggca gcaagatttc cagccttttg acggtgggcc
     2881 tgtgcaatcg ttatcagatg cgtatagcta ttattcagtg ctgctattga tagcagctga
     2941 aagcgagata tccgtaccag aatggctcgc tcattctgag ggaacgacgg taattttgat
     3001 tgcagaacac aatgcccgtt tgctgaatgg tccggctggt ggggctgtag cattaatcca
     3061 ttatctgaat gagatctgtc atgccagaga aaccgctgtc aatgacgcat tttgtcggac
     3121 gttaacacga ggaagtctgg ctattccgca atgccatctt ctttcgtaat ggattgagaa
     3181 tgctggattt ttaattatat tacgcgcagt attaatcgcg taaatgtttt gcggtcagca
     3241 acggtggggg atccattgtt ctgttcaatg aataccatca tacaattgaa tcaggagtcg
     3301 gtatgatgat ttttgtgacg gggggcgcac ggagcgggaa gagtcgccac gcagaggcgc
     3361 ttattgggga ctcttcacag gttctgtata tcgccacctc gcaaatcctt gatgatgaga
     3421 tggctgcacg gatagaacat catcggcaag gccgcccggc tcactggcgc acagttgagc
     3481 gctggcaaca tcttgatgaa ttaattcatg cagacattaa cccgaatgag gctgtgttgc
     3541 tggaatgcgt taccacaatg gtgactaatc tgttgtttga ttatggcggc gataaagacc
     3601 ctgatgaatg ggattatcag gcgatggaac aggcgattaa tgctgagatt cagtcgttga
     3661 ttgctgcctg ccaacgttgc cccgcaaagg ttgtattagt gactaacgaa gtgggaatgg
     3721 ggattgtgcc ggagagtcgt ctggcacgac attttcgtga tattgccggg cgggtaaatc
     3781 agcagttggc cgctgcggca aatgaagtat ggctggtggt ttcgggtatt ggagtaaaaa
     3841 tcaaatgagt aaattatttt gggcgatgct ctcatttatt acgcgtctgc ctgtaccgcg
     3901 tcgctggtcc cagggactgg atttcgagca ttattctcgc ggtattatta cttttccttt
     3961 gattggatta ttgcttggcg cgattagcgg gctggtcttc atggtgctgc aggcatggtg
     4021 tggcgtacca ctggcggcac tgtttagcgt acttgtgctg gcgctgatga ccgggggatt
     4081 ccatctggac ggcctcgctg atacctgtga tggcgtattt tctgcacgta gccgcgatcg
     4141 catgttggaa atcatgcgtg atagccgttt aggcacccac ggcggtctgg cattgatttt
     4201 tgtggtactg gcaaagattc tggtgttaag cgagttggct ctgcgtggcg aaccgatcct
     4261 tgcatcgctg gcggcagcat gtgcggtcag ccgcgggact gccgcattat tgatgtatcg
     4321 tcatcgttat gcgcgggaag aagggcttgg caatgtgttc attggcaaaa ttgatgggcg
     4381 acaaacctgc gtcacactcg gcttagccgc tatttttgct gccgtattat tgccgggtat
     4441 gcatggtgtg gctgctatgg tggtgacgat ggtggcgatt ttcatcctcg gtcagcttct
     4501 caaacgtacg ctgggcgggc aaacgggcga tacgctggga gcagccatag agcttggtga
     4561 actggtcttc ctgctggcac tgctataaag agacgtgaca tgcaaatact tgccgattta
     4621 ctgaatacga tcccagccat cgatcctgcc gctatgtcgc gtgcacaacg gcatattgac
     4681 gggttactca aacctgttgg tagcctggga aagctggagg cgcttgccat acaactggca
     4741 ggaatgacgg ggttgaatgg cataccgcat gtgggcaaaa aagcggtact ggttatgtgt
     4801 gccgatcacg gcgtctggga ggaaggggtc gctatttctc caaaagaagt gacagccata
     4861 caggctgaaa atatgacccg tggaacaacc ggcgtgtgtg tgctggcagc acaagcgggc
     4921 gctaacgtcc acgtaattga tgttggtatt gatactgctg agcctatccc cgggcttatc
     4981 aacatgcgtg tcgcacgagg tagtggcaat attgcttcag ctccggcaat gagtcgccgt
     5041 caggctgaaa agttgctttt ggacgtcata tgttatacgc gggagctggc aaaaaacggt
     5101 gtcacgctgt ttggtgtagg tgaactgggg atggcaaaca cgacaccggc agcggcaata
     5161 gtcagcacaa tcactggccg ggatcctgaa gaagtggttg ggattggcgc aaacctgccg
     5221 acagataaac tggctaataa aattgatgtt gtgcgtcggg cgattacgtt gaatcaacca
     5281 aatcctcagg atggtgttga tgtcctggca aaagtgggtg gatttgattt ggtcggaata
     5341 gctggagtga tgttaggtgc tgcttcctgc ggtttacccg tgttgctgga tggatttctt
     5401 tcttatgctg ctgcgctcgc agcctgccag atgtctcctg caatcaaacc gtatctcatt
     5461 ccttctcact tgtcggcaga aaaaggcgcg cgtatagcgc tctcgcattt ggggctggag
     5521 ccttatctca atatggatat gcgtttaggt gaggggagtg gtgcagctct ggcgatgtcc
     5581 atcatcgaag ctgcttgtgc gatatacaac aacatgggcg aacttgctgc cagtaatatt
     5641 gttctaccgg ggaatacgac ttctgatttg aacagctaaa aacgaaaagt ctgataaaag
     5701 ctatacttat cggatagtat tcgcgactta ggaaagggac atgatgcgtc gtgtaaatat
     5761 tctttgctca tttgctctgc tttttgccag ccaaaatagc ctggcggtaa cttatccatt
     5821 acctccagag ggtagccgtt tagtggggca gtcgcttact gtaactgttc ctgatcacaa
     5881 tactcagccg ctggagactt ttgccgcaca atacgggcaa gggttaagta atatgctgga
     5941 agcgaatccg ggcgctgatg tttttttgcc gaagcctggc tcacaactca ccattccgca
     6001 gcaactgatt ttgcccgcca ctgttcgtaa agggattgtg gttaacgtcg ctgaaatgcg
     6061 tctttattac tatccgccag tcagtaatac agtggaagtc tttcctattg gtatcggcca
     6121 ggctgggcga gaaaccccgc gtaactgggt gactaccgtt gaacgtaaac aagaagcacc
     6181 aacctggacg ccaacgccga acactcggcg cgaatatgcg aaacgagggg agagtttgcc
     6241 cgcatttgtt cctgcggggc ccgataaccc catggggctg tacgcgattt atattggcag
     6301 gttgtatgcc atccacggta ccaatgccaa ttttggtatt gggctccggg taagtcaggg
     6361 ctgtattcgt ctgcgcaatg acgatatcaa atatctgttt gataatgttc ctgtaggcac
     6421 tcgtgtacaa attattaatc agccagtgaa atacacaacg gaaccggatg gttcaaagtg
     6481 gctggaagtt catgagccgc tgtcgcgcaa tcgtgcagaa tatgagtctg accgaaaagt
     6541 gccattgccg gtaaccccat ctttgcgggc gtttatcaac gggcaagaag ttgatgtaaa
     6601 tcgcgcaaat gctgcgttgc aacgtcgatc gggaatgcct gtgaatatca accgtaacgg
     6661 attttgacat tcaaaacgtc tggtttgtcg gtctgtgtaa gcattccgtc atccagaaca
     6721 ggttgtttcg aatcatacaa atgataattt tcttctcata tgcgcatctc ttatgagcat
     6781 aaacgttata ctcttctgat ttatacatgt ttgcggatga catgatcaat ggaaaagaga
     6841 atgacgaaac agaataaaaa cagccgttgg cagggttacg atcctcgtta catatactcg
     6901 ctcgtggtca gacgctattt tgcggatatg aaaaccaaga ttgagattgc tgaggagctt
     6961 ggcgtttccc gctttaaagt tgccagattg atcgatgagg cgatcgaaca ggattatgtg
     7021 aggtttatct tccctaagca gcaagcgatg gatgaagaaa tcgccaataa tctacggaaa
     7081 aaatttcatc tggaagatgc aattgttctt tcagttgctg aatcatggac gacgcaagaa
     7141 gaactcaatc acaaattggg tgaagtcacc gctgaatatc ttatgcaatc tcttcatgaa
     7201 ggtatgaaag tggggatcgc ctggggacgt gtattatcaa gtacggtcag taagttgagc
     7261 aagctgcctc ctttagacgt tgtgcagtta tctggcgtac atccggggat agagtttagt
     7321 caagggccaa tagatcttat tcataagatc gctgccattt cgcagggaaa agcgcaccca
     7381 atgtacgtgc cgatgtgggt cgatgacgaa gagcttgctg ccagactggc aggtgatcca
     7441 gcggtattag atacacagca atattactcg cagttggatg tggttatcac cgggataggt
     7501 gactggaaat caggttcttc aagcttgtgt aaaatatttc cggatacctg gtgcgaagct
     7561 ttgtttcaac aagatatcgc tgcggatgtg tgtatctcgt tggtcagcag ggaagggaag
     7621 attcttcata gccctattga acgtctggga tttggcattt cgacggatca actacaaaaa
     7681 gccaaaaaag tgattggtgt cgctggaggg gaggaaaaat atgaaggcat tcttgcttcg
     7741 ctgaaatctg gacttttaaa cgtcttaatt actgattttg atacggctat taaacttctg
     7801 gattaatagg cgtatcagtg aagaaagggc agtaccgctg attctgccct tttaatttca
     7861 ggatgctgcg agagtatcct ctacatattt agctacgcgc gttgcaggta aattgcggca
     7921 agtctcaaca aaggcgttat cctgaaacag tgtgaataaa cgacgcaata tcgacatatg
     7981 ttgatcactg gtggcaaatg ccaggttaaa cataatgcta caatcaactg ttaaatcagc
     8041 atcttccatt tgcccgaaag tgacatcatt ggcgaaacga acaatactga tactgtttac
     8101 atttaccagc gtgtaatcgg catggggaat ggcgataccc tgaccgtttg ccatcattag
     8161 ccctgtcgga tagctttttt cacgttccag gcaggcgtca atgtattctg cagtgatgta
     8221 gcctttttcc aataattgcg taccagcaaa ggtgatcgca gtttgatagt cactgatgct
     8281 gttatcaaca tcattcacca gaatttcaaa tttcattgca cacctgttat ttcaacatct
     8341 cttctttaac tgcagcgaaa atctcatctt cgcccagacc agtcagtaat ccagcgccaa
     8401 cgatgacacg agtagtaatg gccgggttat cgagtttggt cgatgaaacc accaggtcat
     8461 agctgttgaa ggtatcgctg gtgcatgcca ttaacgaaga tttatcgact tgcgcttcaa
     8521 ttttctcttc tttcaggaag tcctgcagta cagagaccat gatggaagat gaggcaa
//
LOCUS       DAZKHK010000174         8533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKHK010000174 DAZKHK010000000
VERSION     DAZKHK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.175"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..251
                     /locus_tag="ACHOS5_004526"
     CDS             <1..251
                     /locus_tag="ACHOS5_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9779239.1"
                     /translation="LNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLI
                     TFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(618..2210)
                     /locus_tag="ACHOS5_004527"
     CDS             complement(618..2210)
                     /locus_tag="ACHOS5_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9779240.1"
                     /translation="MSQKYLIRIAELESQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPAHLPREIHRLESEESCCPECGGELDYPGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARKGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLAPGQKKAK
                     TGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAFTGYDRLF
                     SAEREGGALTEVACWAHARRKIHDVYISSKSAKAEEALKRISELYAIEDEIRGLPESE
                     RLSVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSV
                     LPEWPSNRVDELLPWNVVLTNK"
     gene            complement(2241..2591)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004528"
     CDS             complement(2241..2591)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9779241.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQYVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRRNSLTML"
     gene            complement(2588..2950)
                     /locus_tag="ACHOS5_004529"
     CDS             complement(2588..2950)
                     /locus_tag="ACHOS5_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9779242.1"
                     /translation="MVKLALQPGASVARIAREHDINDNLLFKWLRLWQNEGRVSRRLP
                     VTTSSDTGVELLPVEITPDEQKEPVVAIAPSLSTTSQTRVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            complement(3066..3272)
                     /locus_tag="ACHOS5_004530"
     CDS             complement(3066..3272)
                     /locus_tag="ACHOS5_004530"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9779243.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRVFVARIAREHDIND
                     NLLFKWLRLWLIKGKRKPTAVCSH"
     gene            complement(3403..6084)
                     /locus_tag="ACHOS5_004531"
     CDS             complement(3403..6084)
                     /locus_tag="ACHOS5_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP9779244.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDGEVMLTCHFRSGKKYTRP
                     LSVCQITPTVILRNVFLERKGNAVTSRAERVIIYGDKYAAVYYRESERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVADERVFYARGNNKNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLMAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQRLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANMIDDVLQQVKKHLNAINQVASLKAEINELSVEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKSIREKQISLNEYEKVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMSFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQQSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIRYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSDDSRFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHRELLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLSG"
     gene            complement(6758..7282)
                     /locus_tag="ACHOS5_004532"
     CDS             complement(6758..7282)
                     /locus_tag="ACHOS5_004532"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP9779245.1"
                     /translation="MAQLSRTPSLLNHASEWITLSGQQITRLTELPPAYNLQRSAQLL
                     QQLRGLFPDNPRVQEMVDNWQKSVRSRALPEEAMTGWNEGMTRLQQLAERLNRLDEQR
                     GKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLIN
                     RYQMEHTGNQERQP"
     gene            complement(7334..7483)
                     /locus_tag="ACHOS5_004533"
                     /pseudo
     CDS             complement(7334..7483)
                     /locus_tag="ACHOS5_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7808..8122)
                     /locus_tag="ACHOS5_004534"
                     /pseudo
     CDS             complement(7808..8122)
                     /locus_tag="ACHOS5_004534"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(8177..8482)
                     /locus_tag="ACHOS5_004535"
     CDS             complement(8177..8482)
                     /locus_tag="ACHOS5_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779246.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVLH"
BASE COUNT         2115 a         2154 c         1910 g         2354 t
ORIGIN      
        1 tgctgaacat gatcgtgatt attcctggtg tggtgccgca cttcttcgta ggtgcagctg
       61 caggggtatt cggtaatgca acgggtggac gtcgtggtgc gattttgggt gccttcgctc
      121 agggcttgct tattaccttc ctgccagtat tcttattgcc tgtgctgggt aacattggtt
      181 ttgctaacac caccttcagt gatgctgact ttggcgcact gggtattttg ttagggatta
      241 tcgttcgcta atattttgat gacattaata tcctccagga tttcaggaac gtggaggata
      301 ttaattcctc ttattttctc catttttctt tcttccagtc aggactgcat ctgattaatc
      361 gtaagcgctc cgggaagtac gtcgcgtaag gattatttta cagctgagaa gtttcagtgc
      421 agcagttcat gcacacggtt cgacggctag tcattgatct tcataattac ctcgcgtaaa
      481 aaacggggta aggccgctca tccagcgtca tctccacagg taataattca acgccaatgc
      541 cagaagagtt ccttaccgga agacgccgtg atatacgccc tttgatcgta agcgtcaacg
      601 gagcaccgta ttgacgctta tttattggtg agtactacgt tccatggcaa gagttcatca
      661 actcggttgg aaggccattc cggcagtacg ctcaggatat ggcgcagata cgcttccgga
      721 tcgataccgt tcagacggca ggtgccgatc agcccgtaca acagtgctcc acgctcgccg
      781 ccgtgatcgc tgccaaagaa cataaagttt ttctttccga gacagactgc acgaagcgct
      841 ctttccgcag cattattatc cgcctccgcc agaccgtcat cactgtaata acagagggca
      901 tcccactgat tcagtacata gctgaacgct tcgcccagtc tggatttttt cgacagcgta
      961 ccattcttct ccaccatcca ttcatgcagc gacgtcagta acactttgct tcgctgctgc
     1021 ctgacggaaa gacgctctga ctccggtaat ccccgtattt catcctcgat ggcgtacagt
     1081 tcactgattc gcttcagggc ttcttctgcc ttcgcacttt tgctgctgat gtatacatcg
     1141 tggatttttc gccgggcatg ggcccagcac gcaacttctg tcagtgcacc accttcacgt
     1201 tctgcactga acaacctgtc gtaacctgtg aacgcatccg cctgcaggat accccggaag
     1261 gggcggaggt gttgctccgg gtgtttcccc tgccggttcg gcgagtacgc gaaccagacc
     1321 gctggaggag atgacgaacc cacattgcga tcatcccgga catacgtcca gatacgccct
     1381 gttttcgcct ttttctgacc cggtgccagt acctttaccg gtgtgtcatc agtgtgaacc
     1441 ttgcgggtgt tcattacata acggtacagg gcatcattca ccggtgtcat taactggcag
     1501 cacgcgtcaa cccagttgga gagtaaggcc cggctcagtt cgacaccctt gcgggcaaag
     1561 atttcactct gacgatacag tggcagatgt tcgcagtatt ttcccgttaa cacgcgggca
     1621 agtaatccgg ggcccgcgat accacgctct atcgggcggg acggcgccgg tgcttcaacg
     1681 atgcagtcac attttgtaca ggcttttttt acccgttctg tgcggatcac tttcagggcg
     1741 ctgctcacca gttccagttg ttctgcactg acttcccctg gataatccag ctcaccgcca
     1801 cactccgggc aacagctttc ttcagactcc agacggtgta tttcacgggg aaggtgtgcc
     1861 ggtaacggac gacgatggcg cgactgtcgc aactggcggg gaacctgtgg atcgtcttcc
     1921 cgcccactgt aacgatcgct gtcctgttcg cgttgtttca gcagggcctc agcctgttca
     1981 acttcacgac gcagtttttc agaacgggta ccgaacagca tccggcgcag tttttctatc
     2041 tgagcccgca gatgttctat ttcccgttca tcttcttcga tcttttcttc ggcacgtgcc
     2101 agtgcagagc gcaggaaggc ctccgtctct tcaaccagac tcagttgctg gtctttctga
     2161 cggagctggc tttccagttc tgcaatgcga atgaggtatt tctgactcat gaccgttttt
     2221 ataatgcggt caggattttt ttacaacatt gtcagtgagt tacggcggga tgtttttggc
     2281 tgacgccagt ccagcttatc gaggagcatt gccagttgcg agcgggtaat ggataccttg
     2341 ccgtcacgta ccgcaggcca gataaactgg ccttcctcca ggcgtttagt gaacaggcac
     2401 agaccatcag catcagccca aagaatttta acggtgtcac cccgtcggcc acggaagata
     2461 aacaggtgac cggagaaggg attatcattc agcacatatt gtacctgttc tcccagtccg
     2521 ttgaaggatt tacgcatatc ggtaacgccg gcaacgagcc agatacgggt acctgatggg
     2581 agtgagatca tcttcccctc ccggtcagtt cacggatcaa caccgtgagc agctctggcg
     2641 atggattttc cagcgtcatg ttaccgtgac ggaattccac cttgcaggag ctggcactga
     2701 ctctggtctg agaggtagtg gataaagatg gcgcaatggc caccacaggt tctttctgct
     2761 catccggcgt tatttctaca ggtaataatt caacgccagt gtcagaagag gtcgttaccg
     2821 gaagacgccg cgaaacacgc ccttcgttct gccagagcct gagccatttg aacaggaggt
     2881 tatcattgat atcgtgttcc cgggcgatac gggcaacaga ggctcctggt tgcaaagcca
     2941 gtttaaccat ttgaagttta aaattatttg aaaatgatct gcggggttct gcggataatg
     3001 ttttttgctc cataacaggc gtccactagt ttgaaaaggt gggcacctac gttaccaata
     3061 ctggcttaat ggctacatac ggcagtcggt ttacgcttac ctttgatcag ccagagcctg
     3121 agccatttga acagaagatt atcattgata tcatgctccc gggcaatacg ggcaacaaaa
     3181 acccttggtt gtaaagccag tttaaccatt tgaagtttaa actcatttga aaatgacctg
     3241 cggggttctg cggataatgt tttttgttcc ataacaggca tccactagtt tgaaaaggtg
     3301 ggcacctacg ttaccaatac tggcttaatg gctacatacg acggtcggtt tacgcttacc
     3361 agaaaactaa tagtgctata tgcaattttt aatattcaga ctttagcctg acaggcctga
     3421 caaaaacgcg cccaacttcc cttctatatc actgtctata gtacgtaatc ttagcaatgg
     3481 cagaccacat ttcttcagta tactgttttt aagttcatca cgttcagcct ggacactggt
     3541 cagatgatag cctccatcca cttcaataac ccctaatgga gtcttcccga ccttatagta
     3601 aacaacaaag tcacaactgg cccgattacg catgaataac agttcacggt gagtaaaatc
     3661 tccccgctcc agcaacacca actgattcag cgctatttgt gaatgaaaca tcatgctccg
     3721 ataactatcc tgcgacagga tatcccgcaa caggcaggcc acaatctgtt ctgacttaaa
     3781 gcgtgaatca tctgaattca atctactatt cagtcgttcc agtgatttgt catactcact
     3841 gtacagcaaa tcaaatgctg atatcaccgg gctctcgaaa atctctccgt catcagcgta
     3901 gtatctgatg taacgaatca gtgccgcaat atgtccagcg tggcgttcaa atacatcatt
     3961 accagtcacc aacgtaaatt tgtttcgggc ccttgaaaca gcgacattca ccagttcagg
     4021 attatcaaca aacgcgatat tccgactttg ctggctgctg cgtttcttat ccagcacagt
     4081 ggaaaacaca atttcatcgc actcccgtcc ctgaaattta tgcaccgtcg atttcacaaa
     4141 atcagcgggc agcactgttt cagccagatt aacctgtgca ttatatgggg cgatatatcc
     4201 cctgctactt tcctcatccc agtaatgtcc ttccagagat tcaagctccc tgagatttga
     4261 aaaattacgg gtatggtttc cttttgccgt gatgatcaga gataatgagg cttcacctga
     4321 atcaagcgtt agcggtatca gagcattatc ataaaactgc ttattacaaa attgaatgat
     4381 tttaggatga cagcgataat gctctttaag aagagttact ggcaccatat tcctgaacga
     4441 catacaaacc gaatcaagta gactatattt ctcacaatta tagtattctg ccggtggaga
     4501 tgggctgttc gggagtaata caggaacatg tggaagttgc tttcgatccc ctacaacgat
     4561 tacattacgg gcacatccta gtcccagaat gcccggaaca atatcctgct gtgaggcttc
     4621 atcaataatc acataatcaa gaagagcccc cttaccgata gagttaataa tagaatgcgt
     4681 gctgctacca ataaccggaa aacgctttat aaaacggtta aattcatccc tgtatgtctc
     4741 tgccgagaat gaatttcttg ttgaaatatt tcttcgcaga tgttgtttta aataaagcac
     4801 agaccatgat gtcaggcgct ccagtaaaat tttaaaatca gattccttta atactttctc
     4861 atattcattc aaagaaattt gtttttcccg gatggatttt tcataataag aaagctgaag
     4921 agaagtaaat agtgccagcc ttctctccgg aatcaccaat ggtttgactt tcagtatcct
     4981 gaagtttaac aacaaatcaa ttctgtttct gaatccaatc cgcctgtcag aaaggtaatg
     5041 aatataagcc atcaggtcag ttgtttttcg tgaggaaagt cggtatttgt gagaaaaaag
     5101 ctcctccggt cgaagattct gactctgcca ttgctgcagg tatttatact caacactcag
     5161 ttcattaatt tctgctttta acgaagcaac ctggttgata gcattcaggt gttttttcac
     5221 ttgttgaagt acatcatcaa tcatatttgc agacggtgag tcaggtaaga cttcctcaga
     5281 acgcgaaatc gacgttgaaa aaaactgttg tctgttctcc gtacttccca gtcgtgccac
     5341 cacatatccc agtcgttgtt tgtccatttt ttcataaaca ttactgacag ctgagttatt
     5401 attggacaaa attgccactg ttttattctg aattaatata ttggcaacaa tattgagaat
     5461 agtctgtgtt ttacctgtcc caggtggtcc ttcaataact gaaatctgtg aggaaaatgc
     5521 ccgttcaacc gccattaact gactctcatt caggccaaac ggaaaaatca acccccatgg
     5581 cgaatcacgt tttttactct gtcctgaaca gtatgcatgc aaggcggtat ccggatgagg
     5641 aactattttc tttatctgtc tgaggatatt ctcatcgata tttttattat tacctcgagc
     5701 ataaaaaaca cgttcgtcag ccaccctgca caagtaatta aacacggcat gctctgtaaa
     5761 tgctgaacac tgttgaaagt caagtccggt cgttttcatg atgtagggtc gttcgctctc
     5821 acggtaataa acagcagcat atttatcacc atagataata acccgttcag cccgggatgt
     5881 tacagcgttc cctttccttt cgaggaaaac atttcgcaaa attacagttg gcgttatctg
     5941 acatacagaa agcggacgag tatatttttt cccgctgcga aaatgacagg tcagcataac
     6001 ctctccgtca tcatttccag cccacagagt ccagtcggta attttatgag tcttattttc
     6061 accgtcaaca caaatcgtga ccatattctc actcagtaat tatcatttaa cttacggaaa
     6121 attgttatga aactcccgat atttacccgg taaaatactt gtcattgttt gaagccctcc
     6181 ctaaaaaatg accaggcatt tgataacccg gtgtatttct cccgatatga gctccggagg
     6241 attttccaca actccgggtg tgttaacata ttcccgccgg ccatatatac attgagttca
     6301 gtcataacag tcaataccag gattacagga acgcgatgct gatacgctgg ttattgaata
     6361 ctggtagccc ctaactacta attcatcacg atactctgta cttactgttt tttacaacca
     6421 ttcccccagg taaaagagtc catttatcat gcccctcttt aacgcctgcc agcataattt
     6481 aagaatggat accgtgggtg gaattaacaa tacctgctca ggccaatgcc atgtaaccgc
     6541 ctcactgaaa aagccagatc atctaaccag gtacagacca acatcgcaaa aattaccatg
     6601 tagcacgaac tgctgtcata tttctgttca ggtatgccgc tatttcgcgg gttttcttca
     6661 cgccataatg cgtttcaagg tagcgaacat cttcgattgt ccatggtgtg cgcgctcttc
     6721 cgggggtatt ttccatttcc atgttttatc tcagccatta tggctgcctc tcctgattgc
     6781 ctgtgtgttc catctgataa cgattaatca gctgattcag tgccatttca gcctgttttt
     6841 gctgctgttc actgccattc tgattacggg cttcatcata gcgacgtaac tgctcttctg
     6901 ccgggatatg ccggttaaaa gcctgcatga tgccaaacac ctccgttttc agttcactga
     6961 ccgtcatgta ttttccccgc tgttcatcca gacggttcag gcgctcagcc agctgctgta
     7021 agcgggtcat gccttcgttc cagcccgtca tcgcctcttc cggaagcgct cggctcctta
     7081 cgctcttctg ccagttatcc accatttcct gaacacgggg attgtcgggg aaaagtcccc
     7141 tcagttgctg cagcagctgc gcactgcgtt gcaggttgta tgccggcggt agttctgtca
     7201 gtcgcgttat ctgctgaccg gaaagggtta tccactctga cgcatgattt aacagtgagg
     7261 gtgttctgct gagttgcgcc atctggtctg ctgaaagcac atccggtagt ggtgccagca
     7321 gcgccctgag ttgctgctcc gccgatgatg gctgtgtcag aaactgccat cccggaatga
     7381 ccacgctgac agtcagcaat gccgcacaga caccagccac aaacggtttc agaaacgtgg
     7441 gcggggattt ctgaagggag ggtgtgggtt ctgcttttgt ttcagaaaca acatacacca
     7501 gacgggagta cggcaggggc tgatgttcct gtacatccgg agcatcagac cggagtgcct
     7561 gagccggaag ggttaccggc gacgatactt caccctgcac aggctgattc tccagacggg
     7621 tgatatatcc cttcaccatg gcttccggta aagccacttt actgctctgt ttaagttcat
     7681 gccgggccag tatgtcactc agcgttttca ggcatgtttc tgtctgatac agcaggggca
     7741 gattgtcacg ctcatccggt ggcatggccc ggagcgtttt ttgcagacga ctgaacagac
     7801 cggtaataat ttccagccgg gcgtgggtat tcagcggcca catcactgac cagtgatgcc
     7861 gggtcagcgc cacaatcaga gccagccctt cattcagacc acttaaccct gctgtatgca
     7921 tccgggcgat ggtgtaccag gataccgtct gtaattccac gccattgtgt tcatacagac
     7981 gcaggcagag cgtctccaca taacgccagt ccacatccgg acgtgccggg tgagtcagct
     8041 tgctgatttc atcgcgcagg gcagcataat ccggtaatga ccgggggtca ttacctgttt
     8101 tcagttttac ttcagaagtc atcggcagat tcagtgttgc tgtatcaaat cataattaat
     8161 attcccggaa agagcgtcag tgtaataccg gtacccggga aaggtattcc accgcacgga
     8221 tgattgccgg tgtcacatcc caccgccgct tcagaaccgc caataactca ctgagtgttc
     8281 tgatataacg ttcagtgaac agtcgctgcc cgtccctggt taaatgaaaa ccggactgat
     8341 tacaggtaat ggtaatatgg caggcatcat taccaatata tacggcttcc tgtggcctga
     8401 atctgacatt ccagtgccag gaatacggag catgattcag ataaaaaagg cgcatctgga
     8461 tgtcattaaa taacgggttc atcattcagc cctcgtttat gtctgttaag cacacggata
     8521 aggggaacca taa
//
LOCUS       DAZKHK010000175         8432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKHK010000175 DAZKHK010000000
VERSION     DAZKHK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.176"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..396
                     /locus_tag="ACHOS5_004536"
     CDS             22..396
                     /locus_tag="ACHOS5_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9779247.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRASIRGRFRDADAYHLDQAFPLLMKQLKLMLTSGELNPCHQYTVTLYAKGLTCEAD
                     TFGSCGYVYLAVYPTLAPATTS"
     gene            443..820
                     /locus_tag="ACHOS5_004537"
     CDS             443..820
                     /locus_tag="ACHOS5_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9779248.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRTRRATG
                     LMTRSNYRMVNDIIRGEHSEAKQ"
     gene            817..1320
                     /locus_tag="ACHOS5_004538"
     CDS             817..1320
                     /locus_tag="ACHOS5_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9779249.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINVVCDNGHS
                     LRVVDESDRASTDCTPPFAALTGIRCSTAHITAKDSAWLYSMSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAEYLPGLPTF
                     DWSPGTT"
     gene            1317..1514
                     /locus_tag="ACHOS5_004539"
     CDS             1317..1514
                     /locus_tag="ACHOS5_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9779250.1"
                     /translation="MKSLTTETALDILITWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREGIRTLRQLQLLHQNR"
     gene            1611..2454
                     /locus_tag="ACHOS5_004540"
                     /pseudo
     CDS             1611..2454
                     /locus_tag="ACHOS5_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(2604..2691)
                     /locus_tag="ACHOS5_004541"
     tRNA            complement(2604..2691)
                     /locus_tag="ACHOS5_004541"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2655..2657),aa:Ser,seq:gga)
     gene            2924..3862
                     /gene="ghrA"
                     /locus_tag="ACHOS5_004542"
     CDS             2924..3862
                     /gene="ghrA"
                     /locus_tag="ACHOS5_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP9779251.1"
                     /translation="MDIIFYHPTLDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGLQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            3917..4654
                     /gene="ycdX"
                     /locus_tag="ACHOS5_004543"
     CDS             3917..4654
                     /gene="ycdX"
                     /locus_tag="ACHOS5_004543"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP9779252.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            4678..5232
                     /gene="ycdY"
                     /locus_tag="ACHOS5_004544"
     CDS             4678..5232
                     /gene="ycdY"
                     /locus_tag="ACHOS5_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP9779253.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            5334..5825
                     /gene="ycdZ"
                     /locus_tag="ACHOS5_004545"
     CDS             5334..5825
                     /gene="ycdZ"
                     /locus_tag="ACHOS5_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9779254.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(5889..6722)
                     /gene="csgG"
                     /locus_tag="ACHOS5_004546"
     CDS             complement(5889..6722)
                     /gene="csgG"
                     /locus_tag="ACHOS5_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP9779255.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(6749..7165)
                     /gene="csgF"
                     /locus_tag="ACHOS5_004547"
     CDS             complement(6749..7165)
                     /gene="csgF"
                     /locus_tag="ACHOS5_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP9779256.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDYGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(7190..7579)
                     /gene="csgE"
                     /locus_tag="ACHOS5_004548"
     CDS             complement(7190..7579)
                     /gene="csgE"
                     /locus_tag="ACHOS5_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP9779257.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(7584..8234)
                     /gene="csgD"
                     /locus_tag="ACHOS5_004549"
     CDS             complement(7584..8234)
                     /gene="csgD"
                     /locus_tag="ACHOS5_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP9779258.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         2160 a         2020 c         2070 g         2182 t
ORIGIN      
        1 attttttgtg aggattttat cgtgtcagac acactccccg ggacaacact tcccgacgac
       61 aatcacgacc gcccctggtg ggggctgccc tgcaccgtga cgccctgttt cggggcacgt
      121 ctggtgcagg agggtaaccg gttgcattac cttgcagacc gcgccagtat cagaggccgg
      181 ttcagggacg cagatgcata ccacctggac caggcctttc cgctgctgat gaaacaactg
      241 aaattaatgc tcaccagcgg tgaactgaat ccctgccatc agtataccgt cacactgtat
      301 gcgaaagggc tgacctgcga agccgacacc ttcggctcct gtggttacgt ttatctggct
      361 gtttatccga cactggcacc cgcaaccact tcataacccc gaatatctca cctttatcac
      421 taccagttac agagaactta agatgaaaac attacctgac acacacgtac gggaggcatc
      481 acgttgcccg tctcccgtca ccatctggca gacactgctc acccgactgc tggaccagca
      541 ctacggcctc actctgaatg acacaccatt cgccgatgaa cgtgtgattg agcagcatat
      601 tgaggcaggt atttcactgt gtgatgcagt gaactttctt gttgaaaaat acgcactggt
      661 gcgtaccgac cagccggaat tcagtgcctg tacccgttct cagttaataa acagcatcga
      721 tattctccgg acccgccggg caaccggcct gatgacccgc agcaactaca gaatggtgaa
      781 cgacatcatc cggggtgaac attcggaggc gaaacaatga aactggccct gacgctggaa
      841 gccgacagca ttaacgtgca ggcactgaac atggggcgca ttgtcgttga cgtcgatggt
      901 attgagcttg ctgaactgat taacgtggtc tgcgataacg gtcactccct tcgtgttgtt
      961 gatgaatctg accgggcctc aacagactgc acgccaccat ttgctgccct gaccggcata
     1021 cgctgcagta ccgcgcatat cacggcaaaa gacagcgcct ggctgtactc gatgtcgcat
     1081 cagacgaatg acaccggcga atcagaatgg attcatttta caggcagcgg atatctgtta
     1141 cgtaccgatg cgtggtcata cccggttctg cggcttaaac gcctggggct gtcaaaaacg
     1201 ttccgtcgtc tggttgtcac acttacccga cgttatggcg tcagtctcat tcatctggat
     1261 gccagcgctg aatatctgcc gggcctgccc actttcgact ggtcaccagg aacaacatga
     1321 aatcattaac tacggaaaca gcgctggata ttctgattac gtggctgcag gacaatatcg
     1381 actgcgaatc gggaattatc ttcgacaacg atgaggataa aactgattcg gcagcactgt
     1441 tgccttgtat cgaacaggcc agggagggta tccgtaccct gcgccaactg cagcttctgc
     1501 accagaaccg gtgagtctta ctcattatct cactcaccag acttcattcc actgacgcca
     1561 gcctgaacag ggctggcgtt ttcatttatc tgcgaaaagg aatatcgatt atgtctgcaa
     1621 tcacagtctc ccgtccggaa gtggccaacg ggcatacgga cgttatctgc tcaaccagta
     1681 tccgccacat tctggctgta cgaaagagta cactgctgca aatcgacaca cttatccggc
     1741 aactggctga aatctcatca atgacagaaa gtattggcgg taaaaccgca ctggactggg
     1801 ccatgaaaca ggattttcgc tgtggttgct ggctgactga gaaaccggaa accgcaatga
     1861 aagccattac ccgcaacctt gaccgtgaaa tctggcgtga cctgatgcaa cgctccggga
     1921 tgctgtcatt aatggatgcg cagacccatg atacatggta ccgatcactg gagtacgata
     1981 attttccgga aatcagtgaa gagaatattc tgagtacgtt tgagcagcta caccagaata
     2041 aggatgaagt gtttgagcga ggagtgatca acctcttcag agggttgagc tggaattaca
     2101 aaaccaattg cccctgcaaa tttggcaata aaattatcgt caacaatctg gtgaggtggg
     2161 accggtgggg attgcatctt atcaccgggc aacaggcaga tagacttgcc gacctggaaa
     2221 gaatgctgca tctgttcagc ggcaaaccga tccccgacaa cagggaaaac ataactattc
     2281 atctggacga tcacatccgg tctcttcagg gaaaagaatg ctatgaagat gagatgttca
     2341 ttattaaata ctttaagaag ggctctgcgc gcatcacgtt caggaagcca gaactggttg
     2401 acagactgaa tgatattatc gcaaaaacac tatcctgaca tgctggcagt atagctttgc
     2461 cagtatgctc aaagctcgga aattgtccga taagatatga tcgtggggca tataaaccgg
     2521 tttcagatgg catacccggt ggcatactgt ctggaaggat aaataaaaaa gcgcatgtta
     2581 aacaataaca tgcgcttgta atctggcggt gaggggggga ttcgaacccc cgatacgttg
     2641 ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac caaattgtcg
     2701 ttcctgtctt gctggaacgg gcgctaattt agggaaatca tgacctgagg tcaacaaact
     2761 ttttaaaaaa atcgctcgat tattcaaact tcaatcaata tgcagtttta ataagcgaaa
     2821 tttgcttttt ttgccaccga ccacggattt gttatgctgg tggtctttgt agatcataac
     2881 gataagtgcg aataaatttc gccaacactc ttcgggagtc agtatggata tcatctttta
     2941 tcacccaacg ctcgataccc aatggtggat tgaggcactg cgcaaagcta ttcctcaggc
     3001 aagagtcaga gcatggaaaa gcggagataa tgactctgct gattatgctt tagtctggca
     3061 tcctccagtt gaaatgctgg cagggcgcga tcttaaagcg gtgttcgcac tcggggccgg
     3121 tgttgattct attttgagca agctacaggc acaccctgaa atgctgaagc cttctgttcc
     3181 actttttcgc ctggaagata ccggtatggg cgagcaaatg caggaatatg ctgtcagtca
     3241 ggtgctgcat tggtttcgac gttttgacga ttatcgcatc cagcaaaata gttcgcattg
     3301 gcaaccgctt cctgaatatc atcgggaaga ttttaccatc ggcattttgg gcgcaggcgt
     3361 actgggcagt aaagttgcac agagtctgca aacctggcgc ttcccgctgc gttgctggag
     3421 tcgaacccgt aaatcgtggc ctggcttgca aagttttgcc ggacgggaag aactttctgc
     3481 atttctgagc caatgccggg tattgattaa tttgttaccg aatacccctg aaaccgtcgg
     3541 cattattaat caacaattac tcgaaaaatt accggatggc gcgtatctcc tcaacctggc
     3601 gcgtggtgtt catgtcgtgg aagatgacct gctcgcggcg ctggatagcg gcaaagttaa
     3661 aggcgcaatg ctggatgttt ttaatcgtga acccttaccg ccggaaagtc cgctctggca
     3721 acatccacgc gtgacgataa caccacatgt cgccgcgatt acccgtcccg ctgaagctgt
     3781 ggattacatt tcgcgcacca ttgcccagct cgaaaaaggg gagagggtct gcgggcaagt
     3841 cgaccgcgca cgcggctact aataaagcat caggtttcct gctatccttg gcgggaattg
     3901 aatacaggag agagttatgt atcccgtcga ccttcatatg cataccgttg ccagcacgca
     3961 tgcatatagc acattgagtg attacattgc ccaggccaaa caaaagggca ttaaactttt
     4021 tgcgatcact gatcatggcc cggatatgga agatgcgccg catcactggc acttcattaa
     4081 catgcgtatc tggccgcgag tggttgatgg ggtagggatc ctgcgcggca tcgaagctaa
     4141 cattaaaaat gttgatggtg aaattgattg cagcggcaaa atgtttgatt cgctggatct
     4201 gattattgcc ggttttcatg agccggtttt tgcgccacat gacaaagcga ccaatacaca
     4261 agcgatgatc gccactatcg ccagcggcaa tgtgcatata ataagccatc ccggaaaccc
     4321 gaaatatgaa atagatgtta aagccgttgc tgaagcagcc gcgaaacatc aggtggcgct
     4381 ggaaatcaat aattcctctt ttttacactc acgtaaggga agtgaagaca attgtcgtgc
     4441 ggtagccgca gcggtacgtg acgctggagg ttgggtggca ttaggctcgg attctcacac
     4501 tgcgtttacc atgggtgaat ttgaagagtg tcttaaaatc ctcgacgcgg tagattttcc
     4561 gccagagcgc attttgaatg tttctccgcg ccgcttactg aacttccttg aatctcgcgg
     4621 tatggcaccg attgcggaat ttgcagacct ttaattacta gtaatggaaa tatattaatg
     4681 aacgagtttt ctatcctctg tcgtgtgctg ggttcgctct attaccgcca accacaagat
     4741 cctttactgg tgccgctgtt taccctgatt cgtgagggga aactggctgc gaactggcca
     4801 ctggagcagg atgagttgct gacacgtttg cagaaaagct gtgatatgac gcaagtctct
     4861 gccgattaca atgcgttgtt tatcggcgat gaatgtgctg tgccgccata tcgtagcgca
     4921 tgggttgagg gcgcgacgga agcggaagtg cgcgcttttc tttccgaacg ggggatgcca
     4981 ttagcggata cgccagccga tcacatcggc acattgctgc tcgcggcttc ctggctggaa
     5041 gatcagtcaa cggaagatga gagcgaagca ctggaaacac tgttcagtga gtatctgtta
     5101 ccctggtgtg gtgcgttcct tggcaaagtg gaggcccatg caactacgcc tttctggcgc
     5161 accatggcac cgctaacccg cgatgctatt agtgcaatgt gggacgagct ggaagaagat
     5221 tctgaagagt aaatgtgatc tgcatcactc ataactgcaa cgagtaattt gttgttgcat
     5281 aaaatgtgtg ctcgatctca ttcatggccg cgttttctgc tatcatgcgc ggcatgaaca
     5341 tacttctctc tattgcaatc acaacaggca ttctctccgg tatctgggga tgggtggctg
     5401 tttccctggg cttactaagc tgggcgggct tcctgggctg tacggcctac tttgcctgcc
     5461 cgcaaggtgg gctgaaaggg ctggcgatct ccgctgcaac cctgcttagt ggcgtggtgt
     5521 gggcgatggt cattatttac ggtagtgcac tggcaccaca tctggaaatt ctcggttatg
     5581 tcataaccgg tattgtcgcg tttctgatgt gtattcaggc gaaacagctg ctgctttcat
     5641 ttgttccggg gacgtttata ggcgcatgcg cgacgtttgc cgggcagggt gactggaaac
     5701 tggtgttacc ttcgctggcg cttgggctga tatttggtta cgcaatgaaa aacagcggcc
     5761 tgtggctggc ggcgcgtagt gcaaagacgg cgcaccgtga gcaggaaatc aaaaataaag
     5821 cgtgaggggc actcacgctt tcgcttaaac cgtaaaatgc cggatgataa ttccggcttt
     5881 tttatctatc aggattccgg tggaaccgac atatggcggt atttcaccag aatgtcattc
     5941 tgccgttctg ctttattttg caaatcccac agaccacggt cgataccatc attaatcagg
     6001 aaaatgaccc ctgtttcgat agccgacatc aggcacagca taacaggttc gttcgaggtg
     6061 taacccactt ccccttccag caagcgctgg tagtcaataa agcggaaaac cccggcctga
     6121 acttcataag aaagtatcgt cttactggtg ttcaccgagg agaggatctc accggtactc
     6181 acattgacga cgcgcaggtt cacggcaatc tgatcgagct ggtattgcgt gtcggcaccg
     6241 atcccaaaat atcttgcccc aaccccgccc gatttgacgt tgctttcata accgataatc
     6301 gaaccttcaa ccatgatatt tgccgccgtt aaagattgca gcgggattcg gttattaatg
     6361 gcaactgtgc cgttttcttg tgccgcacga ataatcttgc gctcgttaag cagattttgt
     6421 aagccctggc gctccagcgg tataaaccag cgagaatctt tcaatgccgt gaccagcatt
     6481 gccgtggcgc tttgcggaac agcagtggag aagttacttg ccgggtaggg tttaaattgc
     6541 ccggtttcgt cctgaatgtt gtataccgaa acaaagatct tacccgtcgg cgctggcaga
     6601 tgggtcaaat ctttgtagct ctgagcacga ggcattaatg tcggtctggc ggcttcttta
     6661 ggcggggcgg ttaagcatcc gctcagtaac atgacggcaa ccaaaagaaa taagcgctgc
     6721 atgattattt tccttatgaa gctggggctt aaaaatcggt tgagttattt tgtaaacccg
     6781 aaacctggat ggtcgaggtt tgtccggttt tacgatctgt cacgttcaac tgcaattgac
     6841 catcgcggtt agcaatatca acaatataat cgttggtcac catgcggccc ggtttaccgg
     6901 tattaatatt cgacagtagc ccacctaaaa tttgtgactg gatggcctga gtaaagttat
     6961 ctaacgctga gggtgtttca ataccatagt catcgttata gctcggatct ttatacgagt
     7021 tttgggcctg ggcgctattt aataaaaaag cgccattatt tgggttacca ccaaagtttg
     7081 gattacggaa ctgaaaagtc attgttccag cccaacttaa tggcgaaata agcatgagta
     7141 gaactactgc atgtttgaca cgcattgcag cctccgaaca attttttatt tagaattcat
     7201 catgcgccaa atcgcccgta cttaataacg cctgatttat ctggcgacga tttagtgctt
     7261 cttcagtttg aatcagtgca aagacgacag ttttttcgaa gtctcttttc aacgggaata
     7321 aaaaagtctg gaaaataacg tcctgattga ccgttatagt gatccagctt ccccatcgtg
     7381 cactgggcct ttcattaatc gttaagttac ccgtatagtc actttcccat ttatcactaa
     7441 aggctcggta aaaatcatgg ccaatagatg aaacagtatg gtcagttaac aatcccggga
     7501 cttctacctc aacggcgtga agattccctg cggcgaacag aaattctgcc gccacaatcc
     7561 agcgtaaata acgtttcatg gctttatcgc ctgaggttat cgtttgccca ggaaaccgct
     7621 tgtgtccggt tttttacagc tatcttcttg aaaaggttat aaagatgcgt tttaaccgta
     7681 ttttcgctga tgaacaacga acgagcgatc tcgttattag acgcgccgat acgcagctta
     7741 ttcaggatct ctttttcccg atgagtaagg agggctgatt ccgtgctgtt ataacgatag
     7801 ttacccgaat gcgtaatcag gtagctggca agcttttgcg taaagtagca ttcgccgcgc
     7861 aggacgcctt gcaacccatt gacaacacgt tcttgatcct ccatggcata aaaaacgccg
     7921 ttgatatgag gccagttttc aatgtcgcgg tacgggtaat cttcaggcgt atttagcaac
     7981 aatattttga tattgttgtt tttcctgctc aaagtatcct gccaataatg gataagcttt
     8041 ttatccgctt ccatcatatc cagaagaata atagagcctg aagatatatc gtccagagaa
     8101 cgttgaatat tatgtaattt tcctgtaatt gccagcgatt gtttaaggtg ctgcaagaga
     8161 gctgtcgcct gcaaagaagg tttagtgatc aacaataatg tatgaccatg aatactatgg
     8221 acttcattaa acatgatgaa accccgcttt ttttattgat cgcacacctg acagctgcct
     8281 ctaaaataga agcaccagaa gtactgacag atgttgcact gctgtgtgta gtaataaatc
     8341 agccctaaat gggtaaaata taaaactaat ggattacatc tgatttcaat ctagccatta
     8401 caaatcttaa atcaagtgtt aaacatgtaa ct
//
LOCUS       DAZKHK010000176         8189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKHK010000176 DAZKHK010000000
VERSION     DAZKHK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.177"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(356..1213)
                     /gene="murI"
                     /locus_tag="ACHOS5_004550"
     CDS             complement(356..1213)
                     /gene="murI"
                     /locus_tag="ACHOS5_004550"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9779259.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1158..3002)
                     /gene="btuB"
                     /locus_tag="ACHOS5_004551"
     CDS             complement(1158..3002)
                     /gene="btuB"
                     /locus_tag="ACHOS5_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP9779260.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGLPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLISSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAGRSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGTYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(3067..3257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3371..4471
                     /gene="trmA"
                     /locus_tag="ACHOS5_004552"
     CDS             3371..4471
                     /gene="trmA"
                     /locus_tag="ACHOS5_004552"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP9779261.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAEKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4511..4870)
                     /gene="yijD"
                     /locus_tag="ACHOS5_004553"
     CDS             complement(4511..4870)
                     /gene="yijD"
                     /locus_tag="ACHOS5_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP9779262.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4870..5520)
                     /gene="fabR"
                     /locus_tag="ACHOS5_004554"
     CDS             complement(4870..5520)
                     /gene="fabR"
                     /locus_tag="ACHOS5_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP9779263.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTTIIPGNVKDE"
     gene            complement(5624..6940)
                     /locus_tag="ACHOS5_004555"
     CDS             complement(5624..6940)
                     /locus_tag="ACHOS5_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFP9779264.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            complement(6983..8149)
                     /locus_tag="ACHOS5_004556"
     CDS             complement(6983..8149)
                     /locus_tag="ACHOS5_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFP9779265.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFSGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
BASE COUNT         2157 a         2221 c         1930 g         1881 t
ORIGIN      
        1 aagaattaaa cttcgtaatg aattacgtgt tcactcctga gacttggtat tcatttttcg
       61 tcttgcgacg tcaagaatcc gtatcttcga gtgcccacac agattgtctg ataaattgtt
      121 aaagagcagt gccgcttcgc tttttctcag cggtgcgggg tgtgcataat acgccttccc
      181 gctacagagt caagcatttt ttgcttttct ctgttgagat tctcaggaga accccgctga
      241 cccggcggcg tgtttgccgt tgttccgtgt cagtggtggc gcattatagg gagttattcc
      301 ggcctgacaa gaggaaattt aaaataattt tctgaccgcg caacattcaa ccaaatcagc
      361 ctaaaactgc cagtttttcg agcgtttcga agccgtaacg ctgtaaaacg ggcaataatt
      421 gttcagcttc tggcgtcatt gccatacaaa aggcaatatt cgcatcggca gattttgcat
      481 ccggggcttc atgttctaac agccaggccg ttcggcgagc aatcgctgcg ccagaatcca
      541 ccagccgggt tccctctggc agcacctgta acagttcttc ttgtagtaga gggaaatggg
      601 tacaccccag tacaacggta tctggcggct ctttcattct taaccacggg cgtaggatac
      661 gttttagcgc atccagagaa acatcttcgc catgcagctt cgcttcagcc aactcaacca
      721 tctctgccga gcccagcatt tctatctggc attcattagc gaaacgcgcg atcagctcat
      781 gagtataaga acgtttaaca gttccgcggg ttgccagtaa tccgacaatg ccatttgccg
      841 tcagacgtgc agcaggttta atcgccggca cgacaccaac aaccgggaag tcgaactttt
      901 cgcgtaatgc aggaagtgaa acggtactgg cggtgttgca agcgaccaca gccagcgcaa
      961 ggggataacg ttcttgcact gcggtgacaa tttccaccac tcgctcaaca ataaacactt
     1021 cgcttttttc gccatacggg aaagcgacgt tatcgaaagc ataaatgtaa tggagatccg
     1081 gtaagagatg ccggatctcg tcatagaccg acaacccacc gacgccggag tcaaacacca
     1141 gcacggtggg acgtggttca gaaggtgtag ctgccagaca aggtgtattc ccgtcctgca
     1201 gtttggtagc catagactgt ctcataatct ttgtcgaaca ggttggctat tttaccacga
     1261 actgtcaggt gagaggtgac cggatacgca accgcaagat cccacaagct cacaccgccc
     1321 attttaacgg tttgataagg ataagatgag taatccttat catagcgagt gcctaaatac
     1381 tgataagtaa tcccccagtc gaagtcatac aactgccagt cgagctggta tttcacctgc
     1441 tgtttagcac ggcgtaacaa cggcgtgtca gtaattgcat tgcgcgcatc aacataatca
     1501 taactcacag tatgcgtcag tggtccggta tcaaaattag cggtcgcctc gacaccctta
     1561 atccgcgctt tcccttcgtt gtaatatttc agggtgtgat catcataatc gatcaagtca
     1621 ctgacatcgt tacgatatcc ggaaatacgc cagttaaccc cagcagttaa gccttcaaac
     1681 gcgccttccc attgtttgct tttctcagga ttcagattcg gattaccgta ggtgccatac
     1741 agttgcccaa gatttggtgc cttataagat gttccgtagg aagcaataaa gcgataacct
     1801 tcgatgaact cccaaccggc gctggtttgc caggtaccat gacgaccaaa ctgagaatta
     1861 tcgtcgctgc gtcctgcgcc ttcaaaagta aaatcgccga cttgttgcag cccagtcaga
     1921 tagatgccgg tattacgttg atcatatcca tcctcaacat aagctgtgcc cggtgccgtg
     1981 ctctgcttct gccagtcaac acccgcacca atattaccgt ggccaatgat gatgttgttt
     2041 gcccactgga cggtgtactg tttcatctca tcgagcgtcg cggacgaatc ataacgacca
     2101 taatgcggat cgtagttgta atctttgcta tggctgtagc tggaaatcag ttgtgattta
     2161 atcagttcgc cgttatagcg cagtccggca tcccaacttt ggctatagag tttacgggta
     2221 tcgaccagtg gcaagccggg agaataatat gcgtcataat tggtacgatt atcatagcca
     2281 tagccgcgca caaagccgct ccagacatca gtaaaattat gctccagcgc gccataaagc
     2341 gttttactta aaaaaccatc gttatctggc tgcgcttgcg ttccggtatt accataggcg
     2401 acaacatcat aaccatgagt atgggcataa tcgcccaaca gcgttacccg tgtcttatcc
     2461 cccagttgtt gctgtgtaga gacatcatag ttctgataac tattgcttcc ccaccctgct
     2521 gaaatttccg ttccggggtg atcgcgcgtc gtgatgatat tcacgacccc gcctattgca
     2581 tctgaaccat aaacagcgga gcgcggccca cggatatatt caacacgctg gacaagcgca
     2641 atagggaact gactaagatc agcagaacca ctcacccccg ccaaattcag gcggacgcca
     2701 tcaattaaca ccaacacatg actggcattt gtaccgcgaa taaaaataga tgagagctga
     2761 cctgaaccgc cgttttgggt gatatccacg cccggaagac ggcgcagcac atcattgacc
     2821 gaggtcgact gccagcggtc gatatcctga cgcgtcacaa cagtagttgg cgcaagcaca
     2881 gtgctgcgcg gttgttcaaa acggttagca gtaacgacga gagtatccgg gctggtatcc
     2941 tgtgcccaag cggaaaatgc cgtgacggaa cacgccgtca gcagcgaagc ttttttaatc
     3001 attgtaaagc atccacaata gaagaaggat gccgcaggtt tcatcaatat tacgcgatga
     3061 tgagaaccag atgcgacgtt ggccggcagg tcttcgggct tggaggggta tctaagatac
     3121 taagagatga tgacttccca ccggatggca gtgtccgcat aacgcaatca tcgcaccttt
     3181 ccttaccgct gcgcgtcagc tccagattcg cactggattc cctattaact cacaggaccg
     3241 gcaagtggat gctacaggtt gtaacaagtt actgtccaga cgtagctcac aaataggaat
     3301 tcatcaagat ctggacatct gatgagcaat ccctacaatc gccgcgtact ttaatttttc
     3361 aggatacatc atgacccccg aacaccttcc aacagaacag tatgaagcgc agttagccga
     3421 aaaagtggta cgtttgcaaa gtatgatggc accgttttct gacctggttc cggaagtgtt
     3481 tcgctcgccg gtcagtcatt atcggatgcg tgcggagttc cgcatctggc acgatggcga
     3541 tgacctgtac cacatcattt tcgatcaaca aaccaaaagt cgtatccgtg tggatagctt
     3601 ccccgccgcc agtgaactta tcaaccagtt gatgacggcg atgattgcgg gtgtgcgtaa
     3661 taatcccatt ctgcgccaca agttgttcca gattgattac ctcactacgc taagcaatca
     3721 agcggttgtt tccttgctgt accataaaaa gctggatgat gagtggcgtc agcaggcgga
     3781 agccctgcgc gatgcactgc gcgcgcagaa tctgaatgtg catctgattg gtcgggcaac
     3841 gaaaaccaaa atcgcgctgg atcaggatta catcgacgaa cgtctgccga tcgcggagaa
     3901 agagatgatc taccgtcagg tagaaaacag ctttacccaa ccgaacgcgg cgatgaatat
     3961 tcagatgctg gaatgggcgc tggacgtaac caaaggctca aaaggcgatt tactggagct
     4021 atactgcggc aacggtaact tttcattagc gctggcacgc aattttgatc gggtattagc
     4081 caccgaaatc gccaagccgt cagtcgccgc cgcgcaatac aacattgccg ctaaccatat
     4141 tgataacgta caaattattc gtatggcggc agaagaattt actcaggcga tgaatggtgt
     4201 gcgcgagttt aaccgcctgc aaggaatcga cttaaagagt tatcagtgcg aaaccatttt
     4261 tgtcgaccca ccgcgcagcg gtctggacag tgaaaccgag aaaatggtgc aggcgtatcc
     4321 acgtattctg tatatctcct gcaatccgga aacgttatgc aagaatctgg aaacattaag
     4381 ccagacgcac aaggtcgaac gtctggctct atttgatcag ttcccctaca cgcaccatat
     4441 ggagtgcggc gtattactga ccgcgaagta aaacccatgc cggatgcgct tgcatccggc
     4501 atgataccga ttactcagca acttcctgct tacggttacg catcttcgcg ccaatccaga
     4561 acaccataat gacggacagt actgccggga agaagttaga gccgatatcc ggatattccg
     4621 cacgaactac cgtgctgtac agcagtacgc cgagaataaa acaagcagct gccagacccg
     4681 gcaagcctac cggcatagtg cgattaagat aacgttgatg caggcagtaa actgtcaaca
     4741 ccagggaaat aatcgggaat acagaaaatg gtacaatgga gctaaacagc gctgcaaaag
     4801 taccattgat cgataagcca gcaaccaacg ctagcagcag cgtacctcta tcttgatttg
     4861 cttgtttcat tactcgtcct tcacatttcc cggaataatt gtggttttct cttgttcacg
     4921 gcgataccag taataagccc ctttcgaaat cattcgcaac tgcagtacca gtcgctcttc
     4981 taattgccgg cgttgctcga cgccgacatc caacgcctcg gcccccgcac tgaagacaat
     5041 cgtcaccatt gcttcggctt gcgcttcagt aaacgcacgc ggcatatggt tttcgagttc
     5101 cagatagtcc gcaagttccg caatgaagtg ctgaatttca cgcgcaacgg cggcacgaaa
     5161 cgcagcggag gtgccggagc gttcccgcaa taataaccgg aaggcgttag gattattacc
     5221 gatgaactcc ataaatgtgg agaccgaggt gcggatcaca ctcccgcctt tggcgatacg
     5281 ctgacgcgcc tgacgcatga gttggcgtag cattaaaccg ctctcatcaa ccatggtcag
     5341 acccagttcg tctacgtcgc ggaaatgccg ataaaaagag gtgggagcaa tgcctgcttc
     5401 tcgtgccact tcacgcaaac tcaggctggc gaagctgcgc tcagcactta attggctaaa
     5461 tgcggcttcc accagcgaac ggcgggtttt ttctttttgt tgcgctctta cgcccatcac
     5521 gatgtctgaa tccttgccaa aagtactgct ggcactatac cagagaatga acatactgga
     5581 ttgctccagt gttgtgaatg aacggtaacg caataaaaca tatttactgc aattctttgg
     5641 ccggttcttt tacgtacaac aacgccagca tcgacagcaa cgtgcctccc cccagatacc
     5701 acgccggagc cgtcagacta tccagcaaca cgatggattg cgtagcgaaa tattgagcaa
     5761 aaccgccaaa gattgccaca ccaatgctat aaaccagcgc aaaacccaat gcccgaatcc
     5821 gtttcggcaa cagttcggaa atcagcaaca tcactggcac accgccaagc gtggtaaagc
     5881 tgaccatcac gaaaactacc gtcagcaaca taccgggact ggggtaattc accaacagcc
     5941 agaatgacgg ccagctacag agaagcacca tcacacggga gattaaaatc agctttttac
     6001 gcccaaccga atcacatagc atccccacca acagcgcacc cacaaacgta attacccccg
     6061 ccagtaacat cgctgcatga gaataatgtt gaggcatcgc cagatacttc gccgcccagg
     6121 tgccgtaata aaagagggag atataggttg cgaccgtgct accaatagcg agtaaaacgc
     6181 cattaacgat agtcgcctta tgttgcagga gcagagaaaa cgcgctttcg ctggtagcag
     6241 cttttttatt gcgcactggc tcaggtacgt cattttccag gctcagacgc aaccagcaac
     6301 cgataggtgc taataacacg ccaataaaaa acggcacacg ccagccccat tctgccatta
     6361 cggtttctga acctgtggcg aacggtagcc atgcgctcag acctaaagca acaaccccac
     6421 cgaaggtcgt tgccagccct tgggtcgcca gtgaccaact gctgtaaaag ccgcgacgat
     6481 ttgccggagc tgactccacc agtagcgaca ttgaagcccc gacttcacca cctgccgcaa
     6541 cgccctgaat tagacgcgcc agcaccagcg ttgcggttcc ccaatagccc gccgtttcat
     6601 acgtcggtgc gatgccgatc atcagtgtac cgaggctcat cagagcaata gtgaacacca
     6661 tcgccggttt acgcccgaag cgatcggccc aggcaccaac gataatccca ccgagcggac
     6721 gcataaaaaa gctcacacca aacgttgcaa atgccagtaa cagcgcgacg ttatgatcgg
     6781 cactgggtgg gaagaaaagc gtactgatat agaccacaaa aaagttgtaa acgccaaaat
     6841 caaaaaattc gagcgcattg ccaaacgccg aggcaaagat cacccgcctg ccgctgggct
     6901 ttttcttcac actcacggtt tcatttacga tcgccgtcat tccatgttcc ttgccaaaaa
     6961 gtgttgttgt gtgttgtttt atttattgca gccaggcttc aaccagtgcg ccccagtagc
     7021 tgcttgccgg gaccaggctt gcatcgttaa agtcgtaacc ggggttatgc accatgcagg
     7081 aaccttcccc gacatcgcca ttaccgaata acaggaaacc gcctttgggt tgggcttcca
     7141 gcatgaaaga gaaatcttca ctgcccatca ggggtttgat gccaaactca gcccgatctt
     7201 caccaaatgt tttgcacgcc acctgccagg cgaagcgcgc catctcctcg tcatttacta
     7261 ataccggtgt accgttaaca tgcgtaacct cagccgtagc gccaaagctg gcagcctggg
     7321 cttgtgcaag agcaggaata cgggtcagaa gaagttgtcg ggtatcgcgg ctaagagagc
     7381 ggacactgat tttcatctca gcactgtcag gaatgacgtt agccgcctca cccgcaacaa
     7441 tgctgccgac ggtaattacc gccgcttcca gcggatcgac attgcgcgac acaatgcttt
     7501 gtaatgcggt ggtgatatgt gcggcaacca gcactggatc gatggctttg tgcgggatcg
     7561 caccgtgtcc gccacaacca cgaaccgtaa tatggaactg atccattgac gccatcagcg
     7621 ccccaggctg gaagaaaaac ttgccgaccg gcaaacccgg catgttgtgc atcccgaaaa
     7681 tgacatcgca ggggaaacga tcaaaaagcc cctctttaac catgatctca ccgccgttaa
     7741 tcatctcttc cgcaggctgg aagataaggc gtaacgtgcc gctaaaacgg cgggtttcgg
     7801 caaaataacg cgcagccccc agcagtatgg tggtatgccc gtcatgcccg caggcgtgca
     7861 ttaaaccagg atgtttactg gcccagggct tgccactatt ttcataaatc ggcaacgcat
     7921 ccatatccgc ccgcaggcca atacttttct ctccgtcgcc cacttttaac gtggctacca
     7981 cgccggttgt cgccagccct tgctcaacct catagcccca ctggcgcagt ttttccgcaa
     8041 ccaaagcact ggttttaaat tcttgtaatc ccagctccgg gttttcgtgg atctgatggc
     8101 ggatctcgcg aagttcttcc tcaaacccac gaatatagtg ttcgatcatc ggctattcct
     8161 ttattcactt ataaacaggc taattaacg
//
LOCUS       DAZKHK010000177         8098 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKHK010000177 DAZKHK010000000
VERSION     DAZKHK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.178"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..588
                     /locus_tag="ACHOS5_004557"
     CDS             61..588
                     /locus_tag="ACHOS5_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9779266.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADAMKKSGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            646..879
                     /locus_tag="ACHOS5_004558"
     CDS             646..879
                     /locus_tag="ACHOS5_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9779267.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            940..2904
                     /locus_tag="ACHOS5_004559"
     CDS             940..2904
                     /locus_tag="ACHOS5_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP9779268.1"
                     /translation="MSVTKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHIREAEGWEWCAGRMEPVGFCREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMLQPE
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKDGAAFMAMMAEKARL
                     AALLPEGWARDMTTFLSLSQDVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQAETETTEYRDDQAEAA"
     gene            2973..3407
                     /gene="psiB"
                     /locus_tag="ACHOS5_004560"
     CDS             2973..3407
                     /gene="psiB"
                     /locus_tag="ACHOS5_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP9779269.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQA"
     gene            3404..4123
                     /locus_tag="ACHOS5_004561"
     CDS             3404..4123
                     /locus_tag="ACHOS5_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP9779270.1"
                     /translation="MSVRSRALVPLSTEQQAAWRAVAETEKRRHQGNLLAEYPYAGAF
                     FRCLNGSRRMSLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4120..4434
                     /locus_tag="ACHOS5_004562"
     CDS             4120..4434
                     /locus_tag="ACHOS5_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFP9779271.1"
                     /translation="MKWRYSLRWKLPSPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            4589..4738
                     /gene="mok"
                     /locus_tag="ACHOS5_004563"
                     /pseudo
     CDS             4589..4738
                     /gene="mok"
                     /locus_tag="ACHOS5_004563"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4680..4805
                     /locus_tag="ACHOS5_004564"
     CDS             4680..4805
                     /locus_tag="ACHOS5_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP9779272.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGDREVAAFLAYESGK"
     gene            complement(5147..6717)
                     /locus_tag="ACHOS5_004565"
                     /pseudo
     CDS             complement(5147..6717)
                     /locus_tag="ACHOS5_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6737..7084)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004566"
     CDS             complement(6737..7084)
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9779273.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKIHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7084..7731)
                     /gene="tnpA"
                     /locus_tag="ACHOS5_004567"
     CDS             complement(7084..7731)
                     /gene="tnpA"
                     /locus_tag="ACHOS5_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9779274.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQVFTDWPLSGEYSCGQLARALFPGRYPSTPTVTQPVKAEKPRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQQPGEHETSLLPVTLTPE
                     PDNKIPDPVQVEQINTPTDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
     gene            complement(7818..8098)
                     /locus_tag="ACHOS5_004568"
                     /pseudo
     CDS             complement(7818..8098)
                     /locus_tag="ACHOS5_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1737 a         2183 c         2445 g         1733 t
ORIGIN      
        1 gcggctcccg aaactcaaca gatgatgggg gctgaaaaca tcaacagaag gagacacatc
       61 atggcagttc gtggaattaa caaggtcatt cttgtcggtc gtctgggaaa agatccggat
      121 gtccgttaca tccccaacgg gggcgcagtg gcaaacctgc aggtggccac gtcagaaacc
      181 tggcgcgaca aacagacggg ggaaatgaag gagcagacgg aatggcaccg cgtggtgctg
      241 ttcggcaagc tcgcggaagt ggcaggtgaa tatctgcgta agggggcgca ggtctacatt
      301 gaaggccagc ttcgcacccg tagctgggag gataacggta tcacccgtta cgtcaccgaa
      361 atccttgtga aaaccacggg caccatgcag atgctgggga gtgcaccaca gcagaacgct
      421 caggcgcaac cgcagcctca gcagaatggg cagccacaga gtgctgacgc gatgaaaaaa
      481 agtggcgcga aaacgaaagg ccgtggacgt aaggccgcgc agcccgagcc tcagccgcaa
      541 ccgccggagg gggaggatta cgggttttca gacgatatcc cgttctgact ggcctgactg
      601 tgacaaccgc cccgtcctgt gcggggcatc accggagaga tgaggatgag cgaatatttc
      661 agaatacttc agggactgcc ggacggtcct tttacccgca aacaggccga agccgttgcc
      721 gcacagtacc ggaacgtctt tatcgaggat gaccacggcg aacagtttcg cctggttgtc
      781 cgtaataacg gaacaatggt ctggcgtacc tggaattttg aggacggtgc cgggtactgg
      841 atgaaccatg tcatccgtga tttcgggatc cttaagtaag agaggtgccg gacggggatc
      901 acgtccggca gacattaact acacaataag gggatagtca tgtcagtaac aaagtctgaa
      961 ccaaaatcta cccgtaaagc aagccgtaaa cctgcaaaaa cgcaggaaac tgtcctgtct
     1021 gccctgctgg cgcagacgga ggaagtgagc gttccgctgg cttcgctgat taaatcaccg
     1081 ctgaatgtgc gcacggtgcc gtattctgtg gagtccgtca gcgaactggc agagtccatt
     1141 aaaggtgtcg gtctgctgca gaatctggtt gtgcatgccc tgccaggtga ccgccacggt
     1201 gtcgccgcag gtggtcgccg gctggcagca ctcaacatgc tggcagagcg tggcatcatt
     1261 ccggctgact ggcctgtccg cgtaaaaatt attccgcagg aactggcgac ggccgcatcg
     1321 atgaccgaga acggtcatcg tcgggatatg caccctgccg aacagattgc cggattccgc
     1381 gcaatggcgc aggaaggcaa aacacctgca caaatcggtg atttgctggg ctattctccc
     1441 cgccatgtcc agcgaatgct gaaactggca gaccttgcgc ctgtcatcct cgatgcgctg
     1501 gcagaagacc gcatcaccac agaacactgt caggcgctgg cgctggagaa cgacaccgcg
     1561 cgtcaggtgc aggtgtttga agccgcctgc cagtcaggat ggggcggtaa accggaagtg
     1621 cagaccattc gtcgtctggt gaccgaaagt gaagtggcgg tggcggggaa cagtaaattc
     1681 cgcttcgtgg gggctgatgc cttctcgcca gatgaactgc gcactgacct gttcagcgac
     1741 gacgagggtg gctatgtgga ctgcgtggcg ctcgatgccg ccctgctgga aaaactccag
     1801 gctgtcgctg aacacattcg ggaagccgaa ggctgggaat ggtgcgccgg acgtatggag
     1861 cctgtcggtt tctgccgtga ggatgccgga acataccgtt gtctgccgga gccggaagct
     1921 gtgctgacgg aggcagaaga agaacgcctg aacgaactga tgacgcgtta cgacgcgctg
     1981 gaaaaccagt gtgaggaatc cgacctgctg gaagcagaaa tgaagctgat gcgttgcatg
     2041 gcgaaggtca gggcgtggac gccggagatg cgtgccggaa gcggtgtggt ggtgtcctgg
     2101 cgttacggta atgtgtgtgt ccagcgtggt gtgcagttgc gcagtgagga tgacgtggct
     2161 gccgacgctg accgcacgga acaggtgcag gagaaagcgt ccgtggagga aatcagtctg
     2221 ccactgctga cgaaaatgtc ttccgaacgc acgctggcag tccaggcggc actgatgctg
     2281 caaccggaaa aatctctggc actgctggca tggacgctct gcctgaatgt gtttggcagc
     2341 ggagcgtaca gtaaaccagc acaaatcagc ctggaatgta aacattattc gctgaccagc
     2401 gatgcgccat cggggaagga cggtgccgca ttcatggcga tgatggcaga aaaagcccgt
     2461 cttgctgccc tgttaccgga gggatgggca cgggacatga cgacgttcct gtcactcagc
     2521 caggacgtgc tgttatcact gctcagtttc tgcaccgcgt gcagcatcca cggcgttcag
     2581 acccgcgagt atggtcacac gtcacgcagt ccgctggact cgctggagag cgccatcggc
     2641 tttcacatgc gtgactggtg gcagccgaca aaagcaaact tcttcggtca cctgaaaaag
     2701 ccgcagatta tcgatgccct gaatgatgcc ggactgtccg gtgccgcacg ggacgcggag
     2761 aagatgaaga aaggcgatgc cgctgaacat gcagagttcc acatgaaaga caaccgctgg
     2821 gtgcctggct ggatgtgtac accacgtcca caggcagaga cggaaaccac ggagtaccgt
     2881 gacgaccagg cagaagccgc ctgaccgcca gtcataaacc atttatcacg ccgccccgcc
     2941 ggagacgggg cggcagcaag ggagataccg tgatgaaaac tgaactgacc ctgaatgtat
     3001 tacagaccat gagcgcacag gaatatgaag atatccgggc tgccggaagt gatgaacgcc
     3061 gtgagctgac acacgccgtg atgcgggaac tggatgcgcc ggacaactgg acgatgaatg
     3121 gcgagtacgg cagcgagttc ggcggctttt tccccgtcca ggtccgtttc actcctgccc
     3181 acgaacgttt tcacctggcg ttatgttcac cgggcgatgt ttctcaggtc tgggtgctgg
     3241 ttctggtgaa tgccggtggt gagccgtttg cggtggttca ggtgcagcgg cggtttgcac
     3301 cggaagctgt cagccattca ctggcactgg cggcatcact ggatgcgcag gggtacagcg
     3361 ttaatgacat catccatatc ctgatggcgg aaggaggtca ggcatgagcg tacgttcacg
     3421 ggcactggtt cccctcagca cagagcaaca ggccgcatgg cgggcggtgg cggagacaga
     3481 aaaacgccgc catcagggaa acttgctggc ggaatatccg tatgcggggg cgtttttccg
     3541 ctgtctgaac ggaagccgca ggatgtcgtt gtctgacctg cgatttttca tgccgtccct
     3601 gaccgcagaa gaactgcacg gcaaccgtct tcagtggctg tatgccatcg atgtgctgat
     3661 tgaaacacag ggcgaggtct gcctcctccc ccttcccggc gatgcagcag agcggttgtt
     3721 cccgtcggtg cggttccgtg tcagggaacg ccgtcgccat aaatctgcgc tggttatgca
     3781 gaagtacagc cgccagcagg cacgggaggc agagcagaaa gcccgtgcgt accaggcgct
     3841 ggtcgctcag gctgaaatcg aactggcgtt ccattcgccg gaaaccgtcg gaagctggta
     3901 tgcacgctgg agtgaccggg tggcagagca tgacctggaa accctgttct ggcagtgggg
     3961 tgagcgtttt ccgtcactgg ccgggatgga acgctggcag tggcaggata tgccattctg
     4021 gcaggtcatc gcagaggcag gtatggcagc aagggaggcg ggtcacgctg tccgtgagat
     4081 ggagcggtgg atggtgccga acaaactccg ggaggcggcg tgaagtggcg ttactcattg
     4141 cgctggaaac tgccttctcc ctgtcctggc gagcatgaac tggtttcaga agtggtggag
     4201 gcggggcagc ctgcaccggc atccgttatg tcacgctggg ttgccggagc agggtatgcg
     4261 gtctgtctgg attttatttc tgaccgcccg gtgcgccgct ggagcgaaga acgtaaggct
     4321 gcggtacggc ggcggaatct ggagaaacgt atcaacaggc atgcaccact gtttgcggat
     4381 gaactgattg cccgtgaact ggctgaacga ccggattatt ttcagggaaa gtgagtgtgg
     4441 tcagcgcgca gggatatggg ctatgatgtg cccggcgctt gaggctttct gcctcatgac
     4501 gtgaaggtgg tttgttaccg tgttgtgtgg cagaaagaag atagccccgt agtaagttaa
     4561 ttttcattaa ccaccacgag gcatccctat gtctagtcca catcaggata gcctcttacc
     4621 gcgctttgcg caaggagaag aaggccatga aactaccacg aagttccctt atctggtgtg
     4681 tgttgatagt gtgtctcaca ctgttgatat tcacttatct gacacgaaaa tcgctgtgcg
     4741 agattcgtta cagagacgga gacagggagg tggcggcttt cctggcttac gaatccggta
     4801 agtagcaacc tggaggcggg cgcaggcccg ccttttcagg gctgatgctg gtctgactgc
     4861 actgaagcgc ctttataaag gggctgctgg ttcgccggtg gcccctttct ccttgctgac
     4921 attgtagcag catgaacaac cagactttca ggaaggtctg gttccctcag ggaacgggaa
     4981 tgaacgtgcg cctgcggcgc ccgttctttt cccccgcctt cgctgtttat ggactgtcag
     5041 aaatcacatc accttccctt tgattattct ctttttcctc ctgttctgac tctgattact
     5101 gctgccctga ttattggtaa gcggctcgcc agaaccgtat tgatatttac tgagaggtca
     5161 gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggccagtcc tggatatgct
     5221 caatgacgta acgcagccat ttttctggtt ccacattgtt cagacggcat gtgccgatca
     5281 gcgagtacaa caccgccgca tgctcgccac cgctgtcgga acccgcgaag agccagtttt
     5341 tccggcctac ggccactccc cgtaaggcgt tctctgcgat gttgttgtcg atttccaccc
     5401 agccattacc gcagtacacg ttcaggccat cccactgttt cagcaggtat gcgaacgctt
     5461 ttgccgtatc cgagtgacgc gacagtgttt tcatctgagt ctgtatccag tcatacagtg
     5521 actgcatcag tggcgcagct ctggcttttc ttgccgccag acgctgttct gctgtacatc
     5581 cccggacttc tgcctctatg gcatacagtt caccgatacg ctgcagggct tccgtggtga
     5641 tgtcggtggg cactcttgca tgcacatcgt ggatttttct ccgggcatga gccatacacg
     5701 cggcttccgt tattctgccg gattcgtata acacccggta accaccgtaa gcatcggcct
     5761 gaagcacacc gctgtaaccg gccagatgat tttgtggatg gatacctttc cggtccggtg
     5821 agtacgcgaa ccagaccgcc gggggcattt ccgaaccggc gttacggtca tcacggacgt
     5881 agacccacag ccgggcggtc cgggttttac cactgcccgg ctcctggacc ggaacgggga
     5941 tatcatcggc atggacttta ccgggcatca gcacatactg gcgcaggatg tcatacagcg
     6001 gctccagcag ttcagcaacg gcaccggtcc agcgacccag cgtggcacgg ctcagctcca
     6061 cgtcctggcg acggtatatt tctgactggc ggtataacgg cagatggtct gcatacttcc
     6121 cggtgacaac atgggccaga agccccgctc cggcataact gcgtgcaatg ggttttgaag
     6181 gcaccggtgc ctgcacaata tggtcgcacc gacaacaggc cagtttcgga cgttgtgttt
     6241 cgataacctt aaaggcgctg ctgataagct ccagctgctc tgacacatca catcccagtg
     6301 gactgagttc accaccacag gccggacagc attcctcttc cggccgatga cccgggtttc
     6361 acggggaagt gtggtcggta acggtttacg ggctgaagac tggcgcaggg cggatggcag
     6421 tgccgggtca tattgttcac ccagcgtttc cgccatttct tcctgaagtg cgctgattcg
     6481 ctcctgtgct tcctgtatct gccgttcggt ttttgcacga agtttttctg agcttttacc
     6541 gaactgcata cgctgcagtt tcgcaaccag cgccttcagc cggttgattt cagaagcata
     6601 agccgccacc ctctgtgaga gcaggcggtt gtattcggcc atctggcgga tggtgtcctg
     6661 ttgcgtctgc aacagtgccc gcaggcgggc gttctcatga gcaagtgagg tctccatatc
     6721 ctcactttac aacgggttat atgcggattc cagcgcgttc cgttcgtttt ggatgtttcc
     6781 agtcgatgcc ttcaagaagc atggacaact gagccggagt aaggtgaatc tttccatcgc
     6841 gggtgaccgg ccagacgaag cggccccgct ccaggcgttt ggtgaagagg cacagtccgt
     6901 cgctgtcagc ccacaacact tttatctggt caccccggcg tccgcggaag atgaacagat
     6961 gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt tgatgccagg ccgttaaaac
     7021 catttcgcat gtcggtgata ccggcaacca gccagatacg cgaaccggca gggagagata
     7081 tcatcagtgg ctgctccctt tcatttcgcg gataagtgtc tgtaataacg ccggtgtcag
     7141 ttcacctttc agcctgagag ttccggccgg cagaaccagc tcgcagcaca gactgtcggt
     7201 cggtgtattt atctgctcaa cctgcacggg gtccgggatt ttattatccg gctccggagt
     7261 taacgtcacg ggaagtagcg atgtttcatg ttcgccgggc tgctgcaggc cgccgttacg
     7321 ccagagatgc cgccagttaa agagcaggtt atcgttgatt ccgttctcac gagctatttg
     7381 tgcgacacag gcaccgggta aaagagtctg ctgaacaagt ctccatttaa actccggtga
     7441 aaatcggttc cggcggggtt tctctgcttt cacaggctga gtcacggtcg gggttgaagg
     7501 atatcgcccc gggaaaagag cccgggccag ttgcccgcag gaatattcac cggaaagagg
     7561 ccagtcagtg aatacctggt gaaatcgcct caccagagaa aaaatggcgg ttttgctgac
     7621 accagtctgc gcagaaatgc gcgggtacga cagtgactcg tcaaatctca gctgtagcac
     7681 acgcaggatc aattccggat tatctgccag taccgccttt cgtgcattca tcttaaatgt
     7741 ccttttactg cgaaaatgga cattagtatc ggaaacagga aaaggaggcg aaagacggtt
     7801 taaatgatgc gcttacgatt attgtctgtt ttgttgttct cctgcctgtg cctttcccgg
     7861 gcgtccccct ggcggggcgg gctgtcgtga acggagccag cggagctgtc tccgccctgg
     7921 cgggcttcca ttcctgacgc aaaaccccgg gtttgcagcg accggaacgg tcgcgccacc
     7981 cttgtcgcgc cgtggcctgc gctcctggca tcccggcaaa aggccgccgg gatgtaaggg
     8041 gcaacgccgt ttcgcaaacc gcgcttcacg tcgttgcggc gggtccctgc ccgttgtt
//
LOCUS       DAZKHK010000178         8048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKHK010000178 DAZKHK010000000
VERSION     DAZKHK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.179"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..600
                     /gene="yebB"
                     /locus_tag="ACHOS5_004569"
     CDS             <1..600
                     /gene="yebB"
                     /locus_tag="ACHOS5_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP9779275.1"
                     /translation="NINYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGHN
                     GEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLRK
                     LYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLG
                     SIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(602..1123)
                     /gene="ruvC"
                     /locus_tag="ACHOS5_004570"
     CDS             complement(602..1123)
                     /gene="ruvC"
                     /locus_tag="ACHOS5_004570"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP9779276.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNVMQM
                     SESRLNLARGRLR"
     gene            complement(1158..1898)
                     /gene="yebC"
                     /locus_tag="ACHOS5_004571"
     CDS             complement(1158..1898)
                     /gene="yebC"
                     /locus_tag="ACHOS5_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9779277.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1927..2379)
                     /gene="nudB"
                     /locus_tag="ACHOS5_004572"
     CDS             complement(1927..2379)
                     /gene="nudB"
                     /locus_tag="ACHOS5_004572"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP9779278.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVARNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2372..4144)
                     /gene="aspS"
                     /locus_tag="ACHOS5_004573"
     CDS             complement(2372..4144)
                     /gene="aspS"
                     /locus_tag="ACHOS5_004573"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9779279.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4454..5020
                     /gene="yecD"
                     /locus_tag="ACHOS5_004574"
     CDS             4454..5020
                     /gene="yecD"
                     /locus_tag="ACHOS5_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9779280.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5017..5835
                     /gene="yecE"
                     /locus_tag="ACHOS5_004575"
     CDS             5017..5835
                     /gene="yecE"
                     /locus_tag="ACHOS5_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9779281.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKEEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5888..6283
                     /gene="yecN"
                     /locus_tag="ACHOS5_004576"
     CDS             5888..6283
                     /gene="yecN"
                     /locus_tag="ACHOS5_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP9779282.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6324..7067
                     /gene="cmoA"
                     /locus_tag="ACHOS5_004577"
     CDS             6324..7067
                     /gene="cmoA"
                     /locus_tag="ACHOS5_004577"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP9779283.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7064..8035
                     /gene="cmoB"
                     /locus_tag="ACHOS5_004578"
     CDS             7064..8035
                     /gene="cmoB"
                     /locus_tag="ACHOS5_004578"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP9779284.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
BASE COUNT         1844 a         2113 c         2032 g         2059 t
ORIGIN      
        1 aatattaatt atcctgctga atatgaaatt ggtgatatcg tctttacatg tataggtgct
       61 accttatttg gtcaaatatc agctgcatca aattgctgga gtaatcacgt cgggatcatt
      121 atcggtcata acggtgaaga ctttctggtt gcagaaagcc gtgttcccct ctcaaccatc
      181 actacgctat cccgttttat taaacgctct gctaatcaac gctatgctat aaagcgatta
      241 gacgccgggc taacagaaca acaaaaacaa cgaattgttg aacaggttcc ctcccggcta
      301 cgcaaacttt accacaccgg ttttaaatac gaatcttcgc gccagttctg ttcaaaattt
      361 gtttttgata tttataaaga ggcgctatgt attccggtgg gtgaaataga gacgtttgga
      421 gaattgttaa atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcttaggt
      481 tctattccgt gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccgggtttg
      541 gtgttgattc acgcggtggg agttgaaacg cctcagcctg aactgaccga ggcggtataa
      601 cttaacgcag tcgccctctc gccaggttca gtcgcgattc gctcatttgc atcacattct
      661 gactaacgtg gcagtgggtg atggcaatcg ccagcgcatc ggcggcatcc gcctgtggat
      721 tagcgggcag tttcagcaag gtgcggacca tatgctgcac ctggcttttt tcggcactac
      781 caatacctac cactgtttgc tttacctgac gtgccgcata ttcaaatacc ggcaattcct
      841 gattcaccgc cgccacaatc gccactccgc gcgcctgccc cagtttcagg gctgagtcag
      901 cgttcttcgc cataaagacc tgttcaatgg cgaaataatc aggctggaat tgggtgatga
      961 tttccgtcac gcccgcatag atgagcttca gacgagacgg taaatcatcc actttggtgc
     1021 gtatgcatcc gctacccagg taggacagtt gcctgcctac ctggcggatg acgccatagc
     1081 cggtcacgcg cgaacccggg tcaatgccga gaataatagc catcacgcgt ctccgttttg
     1141 ctgtttagca ggcctcatca gagagtcgct gcaacctcat cagagatttc accgttatgg
     1201 taaacttcct gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggt
     1261 gcggtttctg catccatatc agctttggtg gacgggatca tggaaacttc cgcgctgtct
     1321 gctttcagac ctgccgcttc cagagcgtcg cgtactttgc ccatttcttc ccatgcagtg
     1381 tagacatcaa tcgcgccgtc atcataggtc acaacgtctt cagcaccggc ttccagggct
     1441 gcttccatga tggtgtcttc atcgcctttc tcgaaggaga tcacgccttt tttgctgaac
     1501 aaataagcta cggaaccatc agtaccgagg ttaccgccac atttgctgaa tgcatgacgc
     1561 acttcagcaa cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg
     1621 ccaggaccgt aaccttcgta gatgatggtt tccatgtttg catcatcatc accgcccaca
     1681 cctcgtgcaa ttgcgcggtt cagagtgtca cgggtcatgt tgttagacag tgctttatca
     1741 attgctgcac gcaaacgcgg gttagcgtcc ggatcaccac cgcccagctt agccgcggtt
     1801 accagctcac gaatgatttt agtgaagatt ttaccgcgct tagcatcctg cgcagcttta
     1861 cgatgtctgg tgttggccca tttactatga cctgccataa aaatatctcc agatagccct
     1921 gcctgttcag gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc tccaggactt
     1981 agtgagcgcc gccgcagcag acgcatcaag ccacttgtaa gccagatgtt cagtgaaaac
     2041 gatctggcgc tcgtgcggga gcgcaagaca gaaccatgat tccgtattac gcgccacgcc
     2101 cggcgcatag cgatgacgta aatgtgaaaa aatttcaaac tctaccgtgc gctgacagtc
     2161 aattaaggtc agttgttcag cgacaacatc aatggtgacc tcttccttta cttcgcgcat
     2221 ggcagcttgc ggcgcggttt ccccctcttc cacgctgccg gttaccgact gccagaaatc
     2281 gggatcgtca cgccgctgca acatcagcac ccgtttcgta tcttgtgcgt agatgaccac
     2341 taagatcgaa acgggacgct tataagccat atcagttatt ctcagccttc ttcacaacct
     2401 gaatgctcag ctcagccagt gcagtcgggt tagcaaagct cggtgcttca gtcatcagac
     2461 acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg gtgccggtca
     2521 gcagcatggt cagacggtca agaccgaatg ccagacctgc gtgcggcgga gtaccgtatt
     2581 tcagggcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg atacccagaa
     2641 taccaaacac cgtctgctgc atatcaccat tatggatacg cacagaacca ccgcccactt
     2701 cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc attttccggt gcagctttca
     2761 gttctgctgc cgtcatgtct ttcggtgagg tgaacggatg gtgcattgcc gtcaggccgc
     2821 cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc catttgcttt
     2881 cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcccccatc gcgtcggcaa
     2941 caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg ccagtacgct
     3001 ccaggatagc ttcaatgatt tctgcattga ggaacttcgc taccgggctg ttgatacctt
     3061 ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg ccgtagattt
     3121 taacgaagtt accgtattcg tcgatctgct tacgggtcaa cgatgcgccg cccggaacac
     3181 gcagagcggc aacacggcct ttcggatcgt tcgccggacc tgcaaatact gcaaactcaa
     3241 cagatttcag cagatcggca acgtcggtca gttccatcgg gttacgcaga tccggtttat
     3301 cagaaccata acggcgttct gcttctgcaa aggtcattac cgggaaatcg cccagatcca
     3361 cgcccttcac ttccagccac agatgacgca ccagcgcttc catcacttca cgcacttgcg
     3421 gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga cggtcagcac
     3481 gcaggtcttc gtcacggaag catttaacga tctgatagta gcggtcaaag ccggacatca
     3541 tcagtagctg tttgaacaac tgcggggatt gcggcagcgc gtagaattta cctttgtgca
     3601 cacgagaagg caccaggtag tcacgcgcgc cttcaggcgt ggctttggtc agcatcggag
     3661 tttcgatgtc gaggaagccg tggtcatcca taaaacggcg caccaggctg gtgattttag
     3721 cgcgggtttt caggcgctga gccatttccg ggcgacgcag gtcgaggtag cggtatttca
     3781 gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct gcacggttga
     3841 tgatagtcag cgaggacgcc agtacttcga tttcgccagt cgccatatcg cggttaatat
     3901 ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat gcagaactca ttacgcagtt
     3961 cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc acgatacctt
     4021 cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg ttgacccaac
     4081 cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa tattctgtac
     4141 gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca ggtgaccaag
     4201 tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat actggaaatt
     4261 ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc gttaccactt tatatcgggc
     4321 ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca taaaaattaa
     4381 caaaatttgt cgttccgcca tcggctaatc gcattaaggt gagaggcacg attttgtttt
     4441 gtcaggagtc atcatgcttg aacttaatgc taaaaccacc gcgctggtgg tgattgattt
     4501 acaagaaggc atcttacctt ttgccggagg tccacatact gccgatgagg tggttaatcg
     4561 cgccgggaag ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc tggtgcgcgt
     4621 tggctggtct gccgattacg ccgaagcatt aaaacagccg gttgatgccc cctcccccgc
     4681 aaaagtgttg cccgaaaact ggtggcaaca tcctgctgca ttaggtgcaa ccgacagcga
     4741 tatcgaaatc atcaaacgtc aatggggtgc gttttacggt acggatctgg agttgcaatt
     4801 acgccgccgg ggtatcgata caatagtgtt atgtgggatc tcgaccaata tcggtgttga
     4861 atccaccgcc cgcaatgcct gggaactcgg ttttaatctg gtgattgccg aagatgcctg
     4921 tagcgccgct agcgccgagc agcacaataa cagcattaat catatctacc cgcgcatcgc
     4981 ccgtgtgcgt agcgttgaag agatcctcca cgcgttatga tttacatcgg tttgccacaa
     5041 tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc ttgaagagta tgcccgccac
     5101 tttaactgtg tggagggtaa caccacgcta tatgccctgc cgaaacccga ggttgtcctg
     5161 cgctggcgtg agcagaccac agatgacttc cgcttctgtt ttaagtttcc ggcgaccatt
     5221 tcgcatcagg cagcattacg gcattgcgat gatttagtga ctgaattttt gacccgcatg
     5281 tcaccgttgg ctccgcgcat tggacaatac tggctgcaac tgcctgccac attcggccca
     5341 cgggagctgc ctgcgctttg gcattttctc gattctcttc ccggcgaatt taattatggt
     5401 gtggaagtcc gccatccaca gtttttcgcc aaagaggaag aggaacaaac gcttaatcgc
     5461 ggtttacatc agcgcggcgt taatcgggtg attttagaca gccgcccggt tcatgcagca
     5521 cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat
     5581 gctgtactga cggcgaaaaa tccactgatc cgttttatcg gtagtgatga tatgacgcaa
     5641 aaccgggaat tatttcaggt ctggttacaa aaattagcgc agtggcatca gaccactacg
     5701 ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt acataccctg
     5761 tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa
     5821 tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg
     5881 agtttgtatg gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa tgaagttctc
     5941 ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggcgacg gcggttttag
     6001 cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc ctatcgcgat
     6061 tgtgttgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg
     6121 catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgttccg
     6181 ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa
     6241 tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt
     6301 tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg
     6361 ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc
     6421 agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct
     6481 tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgcttt
     6541 cagtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac aactctccgg
     6601 cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctact ccagtagacg
     6661 ttattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt
     6721 ttaccctgca attcctggaa ccttccgagc gtcaggcgtt actggataaa atttatcaag
     6781 ggctgaaccc gggcggtgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag
     6841 ttggtgaatt gctgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac
     6901 tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tctgtggaaa
     6961 cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct
     7021 ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa
     7081 cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca
     7141 gattgctaac tggcagcgcg agcagcagca cgggttgttt aagcagtggt ccaacgcggt
     7201 ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga
     7261 aagcgaagag ccactgagcg ccgggcaaat taagcgcatt gaaacgctga tgcgcaacct
     7321 gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg
     7381 ttccgactgg aaatgggatc gcgttctgcc ccatctttct gatttaaccg ggcgcaccat
     7441 tcttgatgtt ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca
     7501 cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa
     7561 actactgggc aacgatcagc gcgcacattt gttaccgtta ggtattgaac aacttccggc
     7621 actgaaagcc tttgataccg tcttttcgat gggcgtgctt tatcatcgtc gttcaccgct
     7681 ggagcatctc tggcagttaa aagaccaact ggtgaatgaa ggcgaactgg tgctggaaac
     7741 gctggttatt gatggcgatg agaacacggt gctggtgccg ggcgatcgtt acgcacaaat
     7801 gcgtaatgtc tatttcattc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg
     7861 ttttgttgat attcgcattg cagatgtgag cgttaccacc acagaagagc agcgacgcac
     7921 cgaatggatg gtcaccgagt ctctggccga ttttctcgac ccgcatgatc cgggtaagac
     7981 ggtggaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc
     8041 tggtaata
//
LOCUS       DAZKHK010000179         7956 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKHK010000179 DAZKHK010000000
VERSION     DAZKHK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.180"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(76..1614)
                     /gene="cadC"
                     /locus_tag="ACHOS5_004579"
     CDS             complement(76..1614)
                     /gene="cadC"
                     /locus_tag="ACHOS5_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP9779285.1"
                     /translation="MQQPVVRVGEWLLTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLDRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1874..1985)
                     /locus_tag="ACHOS5_004580"
                     /pseudo
     CDS             complement(1874..1985)
                     /locus_tag="ACHOS5_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2497..2823)
                     /locus_tag="ACHOS5_004581"
     CDS             complement(2497..2823)
                     /locus_tag="ACHOS5_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9779286.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(2820..3083)
                     /locus_tag="ACHOS5_004582"
     CDS             complement(2820..3083)
                     /locus_tag="ACHOS5_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9779287.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(3155..4021)
                     /locus_tag="ACHOS5_004583"
     CDS             complement(3155..4021)
                     /locus_tag="ACHOS5_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9779288.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAIKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINLFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKENYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            complement(4106..4303)
                     /locus_tag="ACHOS5_004584"
     CDS             complement(4106..4303)
                     /locus_tag="ACHOS5_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9779289.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(4315..4803)
                     /locus_tag="ACHOS5_004585"
     CDS             complement(4315..4803)
                     /locus_tag="ACHOS5_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9779290.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(4800..5177)
                     /locus_tag="ACHOS5_004586"
     CDS             complement(4800..5177)
                     /locus_tag="ACHOS5_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9779291.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     NERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            complement(5267..5635)
                     /locus_tag="ACHOS5_004587"
     CDS             complement(5267..5635)
                     /locus_tag="ACHOS5_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9779292.1"
                     /translation="MSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRADIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCDAD
                     TLGSCGYVYIAIYPTPGTKT"
     gene            complement(5685..6329)
                     /locus_tag="ACHOS5_004588"
     CDS             complement(5685..6329)
                     /locus_tag="ACHOS5_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFP9779293.1"
                     /translation="MSRIITSTVYTLNELSATAREKARDGYRQHHADSNWYENVYEDF
                     RKVCDIFGIDLRQRVFQLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQSATARIIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEIHHRGRYCHPYSMDITVTRNSPTGQIMT
                     TDAEAAVSEALRDLAFWLYRQLENEYDWLTSDATVDEALLINEYTFTEAGLRAG"
     gene            complement(6348..6569)
                     /locus_tag="ACHOS5_004589"
     CDS             complement(6348..6569)
                     /locus_tag="ACHOS5_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9779294.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6632..7108)
                     /locus_tag="ACHOS5_004590"
     CDS             complement(6632..7108)
                     /locus_tag="ACHOS5_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP9779295.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7124..7609)
                     /locus_tag="ACHOS5_004591"
     CDS             complement(7124..7609)
                     /locus_tag="ACHOS5_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9779296.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVASLVPDEQRISFWPQHF
                     GLIPQWVTLESRVFGWMDRLYEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(7664..>7956)
                     /locus_tag="ACHOS5_004592"
     CDS             complement(7664..>7956)
                     /locus_tag="ACHOS5_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9779297.1"
                     /translation="PARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAY
                     QTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         1910 a         1870 c         2004 g         2172 t
ORIGIN      
        1 aacatattat accaacggga acatacaaaa actcaacaac aaatattccc gagcatgaat
       61 caacccggga gttacttatt ctgaagcgag aaatttgtcg agataaggta caacataagg
      121 aacagaagtc tggaatatac cattttcaat ccagtaaagg gtgtttgccc ctgggcgtaa
      181 attaaaggcg gtgagatatg catcagctgc ttcccggttc atccccttca tttcataaac
      241 cttgccaagc aacacataat ttagccagga catttcaaga tcaatgccgg tatttatcgc
      301 ctgataagac tcatctgttt taccttttac cagtgcactg accgctttta tttgatatat
      361 aatggacagg ttgttcaatt ccggcagtgt aacaatgtta tctatttctg tgttcagtgc
      421 tgctaattgt ttttcgtcta aaggatgttg agaatggcgc acgatatcaa ctaatgcttt
      481 ttctgctctc gcgtaggtaa attctgggga tgattgaaca atctcaccta ataattcact
      541 ggcacgatcc aatgatttat catcaccatg cagtaaataa tcatgtgcct gataaaaatt
      601 agttaataac gcaccacgat gcggcaaaat ttgctggagc atctcctgca ttcgttgtgg
      661 ccacggttgg tttaacgctt ttgataaact ctggagcaaa tcattttgaa tcgccagctg
      721 attaccgtta gtgatgacat aacgtttatc cagcatggtt gaaccatctg cattgtctac
      781 caattttacc gacataaagc attgttgagc acggtattgg cgctgattaa caaacgcaat
      841 agataatgtt ttaccggaac tgctcggttc atcaatgttg tagttgattt tgtcatgcac
      901 cataaaggtg gagaaggtgt taagtgatgt cgccaccaaa tcacccacgc ctatcgcgta
      961 agagagctga tacggagaac tccagctgtt acaactctta ttaaccatat taatgtcaat
     1021 atcgcgtgga ttgagcaaaa tgcgcgattt actcatagga agacgtgttt caagacttga
     1081 aaacgctacc agtgctacac agatacctaa cgacaacagg aaaaaaaacc atacccaaaa
     1141 ggtagtgaat cgtttgcttt taactgggga ttgttcaggt ggcgttgtgg tgttttgaat
     1201 gttaagactg tgggagggag aatctgtggc aggaaccgcc tctggtatag ggggaggcga
     1261 agatagcatt atttcctctc cctcttcttc gctgtaccag ataaccggca ccattaattt
     1321 atagccgcgc tttggtacag tagcgatata gacaggacta tcttcatcat tatcttttaa
     1381 tgacttacgt agttctgaga tactctgcgt cacaacgtga ttggtgacaa tacttctctt
     1441 ccagacatta tcgataagtt catccctgct aagtacttcg ccactgtgtt gagcaaagaa
     1501 aaccagaaga tcgattaatc tcggctcaag ggtaagttga cgcccattgc ggctaatttg
     1561 gtttatggac ggagtaagaa gccattcgcc aacgcgaact acaggttgtt gcataataga
     1621 aactcattcg aaaagggaat gatgcaatga taattgccat aacctatttt ttccatctat
     1681 agatgggttt atttacatat tattggtgaa tgcaagacgt tatttttact cgccataaac
     1741 ttcttgatta catagtatta cgaaaggatt ttactgagaa ccagaagtaa ttttccttac
     1801 catcaaaatt catcatcttt gccaaagaaa aatgttcaga aaataatcca tggaaaattg
     1861 tccggagcgc ttactatttt aatggattgt tagtctttgc atgagcaaac ggactgatac
     1921 atttctcttt gttctcattc agaaaatctc atcagttggc gttctgcccg atgttgtgct
     1981 ttatgggtgc gttacgctca tgatgacgtc atttgacgtt caacagcatc acggggccgc
     2041 acgacatttc acgtcagtca gcgctatagc tcagaaacaa attttcccga attgggatat
     2101 gcccgcaaat tgctggtgat gtggggagaa tctggttgag ttcggtagaa ttgatttggc
     2161 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
     2221 tatatgaatt tggtgcccgg actcggaatg aaactggtta tataaccaat tgttttgtat
     2281 tgattgtttt gtctttgttg ttttcgaggc caccaatgag gcccccatgt gcaattcgaa
     2341 ttcttttgac gctataatcc ctcttttttg tgctttgctg taatcatttt gtctggttcg
     2401 atatgctgaa ttgcggaatc gaagtaacaa caaaatacag agatttataa aatacctggc
     2461 tacctgtgtt agggagcgtt aacgcacaga ttttgtttat ggctgccaga aatgatctga
     2521 tggtttgtcg tctggctctg caagtaccct gttgatatcc cgggaaccat gaacgatacg
     2581 ttcgatacga acttcagtat ccagtaccct gaacagcaga aggtaacgac catacgaaca
     2641 acctctgatc ccagccccta attcgggtct ttctctgtag agtactggcg attcggctat
     2701 caacagacat tgttggtaaa gctcttcggt aaaactgatt gcacggacag gattatcctg
     2761 agcgatccag tcacctatag cttcaagatc gtgttctgct aatggtgaaa cagtcagttt
     2821 catattggct gttcaccttc tgccatcttt ttgtatttgt gcgtcagtcg cccgaatacc
     2881 tcttcagcac ttttactttc accgctgttg atgcctgcgg taacggcgct ctggagtgat
     2941 tcaagcttta tctgctgttc ccgttcttcc agagcacgaa gtccggcacg aatgacttca
     3001 cttgtattgt tataccgacc actttctatt tgttcgcgga taaacgtttc aaagtaagga
     3061 ctgagggata cactggtagg catggtgatt ttctcgccga ttgattgcta tcaattaata
     3121 atagttaata ttcgcgaatt gtaaaatacc tcttttatgc cttgcggctt ttgttcactg
     3181 tgcttgccag ggcttccggg tagtgtctgg caataatatc attaagcctg tcaaccagtt
     3241 ctggtttcct gaacgtgatg tgtactgagc ctttcttaaa gtatttaata ctgaacatct
     3301 cgtcttcata gttctcttta ccctgaacag actggatatg atcatccaga cgaagggtga
     3361 tgttttcccg gttgtcgggg atcggtttgc cgctgaacag gtgtagcatt ctttccagat
     3421 cggcaagtcg atctgcctgt tgcccggtga taaaatgaaa cccccaccgg tcccacctca
     3481 ccaggttgtt gacgataatt ttactgccaa atttacaggg tgagttggtt ttgtaattcc
     3541 agctcagtcc cctgaagagg ttgatcaccc ctcgctcaaa cacctcatcc ttattctggt
     3601 gcagttgttc aaatgtgctc agaatgtttg ccgcactgat ttccggaaaa ttatcgtatt
     3661 ccagtgaccg gtaccatgta tcacgtgcct gtgcatccat taatgacagc atcccggaac
     3721 gttgcatcag gtcacgccag atttcgcggt caagattccg ggtaatggct ttcattgctg
     3781 tttcgggttt ctccatcagc cagcaaccgc agcgaaaatt ctgttttatg gcccagtccg
     3841 gggcggtttt acctccaata ctttctgtca ttgctgagat ttctgtcagt tgccggataa
     3901 gtgtgtcgat ttgcatcagt gtggtctttc gtacagccag aatgtgtccg attgaggtgg
     3961 aacagataac gtccgtatgc ccgttgacca cttcaggacg ggagattgtg atacctgaca
     4021 taatcgatat tcctttttgc agataaatga aaacgccagc cctgttcagg ctggcgtcag
     4081 tggaatgaag tctggtgagt gatatttacc ggttctggtg cagaagctgc agatgacgaa
     4141 gagtacggac atcctcccgg gcctgttcga tacagggcaa cagtgctgcc gaatccgttt
     4201 tatcctcatc gttgtcaaag ataattcccg attcgcagtc gatattgtcc tgcagccacg
     4261 caatgaggat atccagcgct gtttcagtgg ttaatgattt catgttgttt ctggttacca
     4321 gtcgaaagtg ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac
     4381 gccataacgc cggataagtg tgacaaccag acggcggaac atttttgaca gccccaggcg
     4441 tttaagccgc agaaccgggt atgaccacgc atcggtacgt aacagatatc cgctacctgt
     4501 aaaatgaatc cattctgatt cgccgaagtc actggtctgg tgtgacagcg agtacaacca
     4561 ggcgttgtct gtttccgtga tatgtgcggt gctgcagcgt atgccggtaa gggttgttaa
     4621 cggtggtggt gtgcaggttg actgttggtc ggagtcgtcc accacacgga gtgaataacc
     4681 gttttcagcg actttgttaa tcagttcagc gagctcaatg ccatcgacgt caacgacaat
     4741 gcgccccatg ttcagtgcct gcacgttaat gctgtcggct tccagtgtca gggccagttt
     4801 cattgttgcg cctccggatg cttaccctgg gtaatgttat ttaccattct gtaattgtcg
     4861 cgggtcatca ggcctgttgc ccggcgagcc cggaggatat cgatgctgtt tattaactga
     4921 gagcgggtac aggcgctgaa tcctggctgg tcagttcgta ccagcgcgta tttttccacg
     4981 agaaagttca ccgcatcaca cagtgaaatg cctgcctcaa tatgctgctc aatcacacgt
     5041 tcattggcga acggtgtgtc attcagcgtg aggccgtagt gctggtccag cagtcgggtg
     5101 agcagtgtct gccagatttc aaccggagac gggcagcgcg atgccgcccg tacgtgtgtg
     5161 tcgggtaatg ttttcataat gaagattttc ctgatatgag cgattaaatt ttgaggagaa
     5221 tggtgctgtg gtgcaccggg cagtagcaga ggtttattct ggtggtttat gttttcgttc
     5281 cgggtgtcgg ataaatggcg atatacacgt aaccacaact gcccagggta tcggcatcgc
     5341 aggtcagccc ttttgcatac agcgtgacgg tatgctgatg gcggggattc agttcaccgc
     5401 tggtgagcat gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
     5461 catccgcatc gctgaaccgg cctctgatgt cggcgcggtc tgcaaggtaa tgcaaccggt
     5521 taccctcctg caccagacgt gccccgaaac agggcgtcac tgtgcagggg agtccccacc
     5581 aggggcggtc gtgattgtcg tcaggatgcg ttgtcccgga gagtgtgtct gacacgataa
     5641 aatcctcaca gaaaatcggt gaatgatagt ttaacgatga cgggtcagcc ggcgcgcaga
     5701 ccggcttcgg taaaggtgta ttcgttgatg agaagggcct catccacagt ggcatctgag
     5761 gtcagccagt cgtactcgtt ttccagttga cggtacagcc agaaggccag gtcgcgcagg
     5821 gcttcgctga cagcggcttc cgcatcggtt gtcattatct gaccagtcgg actgttccgg
     5881 gtgacggtga tatccatgct gtacggatgg cagtagcgtc cccggtggtg gatttcagcc
     5941 tgcagttgcc agaagttacg cttctgcacc gcctgcaggg catccgcgat acgatgaagc
     6001 tcacggtcct gcggggcata ctcccggatt atcctggctg ttgctgactg ccagtgccag
     6061 cgtccttcaa aacaggctcc gtccccctgg ctgcagaatc cgctgaacca gatgcacggc
     6121 tcttccataa acctgccgtt gctgagctgg aaaacgcgct ggcggaggtc aatcccgaaa
     6181 atgtcacaga ccttcctgaa gtcttcatac acgttttcat accagttgct gtcagcatga
     6241 tgctgacggt acccgtcgcg ggctttttcc cgtgcggtgg cggatagctc attcagtgta
     6301 tacacggtgg aggtgataat tctgctcatc tgaatgcccg tcctgattca gttcagggtg
     6361 acgctcatca gccggacata cgggccaaaa ctgtccttac ggcgttcagc aaacacggcc
     6421 agcacaccgg gaatatcctg cacttcacga ccggtatacg cctcagcact gccgtgccag
     6481 cggtatttgc cggtacagaa aggaaaaaga cgggatgccg gatgttgttg gtgaatacgc
     6541 atggcttcac cacgggtgat gattttcata gtgtgatacc tctgaagaca gaagataaaa
     6601 gtgaaaacaa gcgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga
     6661 aaacctggtt gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg
     6721 cctgtaccag acgttccgtg ataaggcggt ctgccttgct gggtgtgact tcaccggacg
     6781 ggtgattgtg cgccagcacc acggcagcgg cattgtggta cagggcgcgt ttaatcactt
     6841 cccggggatg gacttccgtg cggttgatgg tgccggtgaa gagggtttca ccggcaatca
     6901 gttgattctg gttattcaga tacagcaccc ggaattcttc acgctccagt cccgccatgt
     6961 tcagaatcag ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa
     7021 gatggcggtc cagggttttc agggcccgct gaatgaggct gcgctcgccg ggcgtcatct
     7081 ctccgggcag aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca
     7141 taatggcgct gcattccgga tgctgcaggg cataatcccg cagccggtaa taatgaaccg
     7201 tcatggcata attctctgta cgacaggcgt gatggctgta cgccatcaga caggcggcaa
     7261 tgccggcggc ttccgggctc atttcagcgc ggttaccgtt catgacgtta aacagcaccc
     7321 atgtttcgtc atcatcgtca tccggttcgg gtgccataaa tgcgccaccg ttgttcaggg
     7381 tgtacagatt ccagatacca ccgcagtagt cttcatacag acggtccatc cagccgaaga
     7441 cccgggactc cagggtcacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga
     7501 tgcgctgttc atcagggacg aggctggcaa ccaactgagg ctggtcattc cctgatgcag
     7561 cggttacgga aacagaagat gtggtggaat tatgtgaaat ggttttcatg aaatgactcc
     7621 ttaaaggaca aataaatgcg tggttttcag ggtaatggct gaatcagcgc aggctctcca
     7681 gcagcgtttc cgccatcacc cacaatgcgc ggttgagctt aatgtcagtg tcaatgctgt
     7741 gaatggcacg ggtatggata cgttttcctt tagcgctgcg acctgaaatt ccgcctttca
     7801 gcatattctc ctgaatggtc tgataagcac tccacaggtc cttaccgtaa tcctcccggc
     7861 gtcgtggcgt cagaatgtcg gcggtggtga cgggctgatg ttcgtcacca taacggtaag
     7921 tcagtgccgc ctgtgccagc gcctggcgtg ccggtg
//
LOCUS       DAZKHK010000180         7890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKHK010000180 DAZKHK010000000
VERSION     DAZKHK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.181"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            300..413
                     /locus_tag="ACHOS5_004593"
     CDS             300..413
                     /locus_tag="ACHOS5_004593"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779298.1"
                     /translation="MTVVLKSLSEELTTREAAARFNISNESVIRHWVKKVF"
     gene            complement(520..2066)
                     /locus_tag="ACHOS5_004594"
                     /pseudo
     CDS             complement(520..2066)
                     /locus_tag="ACHOS5_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612334.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2430..2652)
                     /locus_tag="ACHOS5_004595"
                     /pseudo
     CDS             complement(2430..2652)
                     /locus_tag="ACHOS5_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011846847.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3758..7873
                     /gene="pic"
                     /locus_tag="ACHOS5_004596"
     CDS             3758..7873
                     /gene="pic"
                     /locus_tag="ACHOS5_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HFP9779299.1"
                     /translation="MNKVYSLKYCPVTGGLIVVSELASRVIKKTCRRLTHILLAGIPA
                     VYLYYPQISQAGIVRSDIAYQIYRDFAENKGLFVPGATDIPVYDKDGKLVGRLDKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYQSVSFGNGKNTYSLVDRNNHSSVDFHAPR
                     LNKLVTEVIPSAITSEGTKANAYKDTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDEQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLPPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIVLKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGTVVLNQ
                     QADTAGNVQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSRLLLDLKAQDTNVSVPIGSISPFGGTGTPGNLYSMILNGQTR
                     FYILKSASYGNTLWGNSLNDPAQWEFVGTDKNKAVQTVKDRILAGRAKQPVIFHGQLT
                     GNMDVTIPQLPGGRKVILDGSVNLPEGTLSEDSGTLIFQGHPVIHASVSGSAPVSLNQ
                     KDWENRQFIMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRVFVDKNDGTGNYV
                     ILEEGTSVPDTVNDRSQYEGNITLDHNSTLDIGSRFTGGIEAYDSAVSITSPDVLLTA
                     PGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNSKITLSGTPVKDTANQYAPAVYLT
                     DGYDLTGDNATLEITRGAHASGDIHASAASTVTIGSDTPAELASAETTASAFAGSLLE
                     GYNAAFNGAITGGRADVSMHNALWTLGGDSAIHTLTVRNSRISSEGDRTFRTLTVNKL
                     DATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMKDPAQGQSLNIPLVTAPAGTS
                     AEMFKAGTRMIGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYKN
                     FMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELDG
                     VDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDYT
                     GNFAGLGTKHYNTHSWYAGAETGYRYHLTEETFIEPQAELVYGAVSGKTFRWKDGDMD
                     LSMKNRDFSPLIGRTGIELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEKR
                     IKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
BASE COUNT         2216 a         1795 c         1933 g         1946 t
ORIGIN      
        1 cgaccaaggc ggtagtgatt aaaggggggg tggatataaa aatttattgt acgtggactg
       61 caccccaaaa cttggagacc caactgatta atgtgcaatt tttatggcaa agccaaaata
      121 ttctctcgaa accagattgg ttgtagttaa tcattatctt tccagacacg atggcgcacg
      181 tcgtatcgct aaacgttttg gtgttgaaaa aacatctgct gggtaaggta agagactggt
      241 aactccacga tattgatcgt atcacatgga aaaatgacct ccacactcct gagtttcgac
      301 tgacggttgt cctgaagtct ctgagtgaag aacttaccac gcgcgaggcc gctgcacggt
      361 ttaatatctc aaatgaatcc gttatccggc actgggtgaa gaaggtcttc tgaacataaa
      421 accaggccgg agcaaagaca tgacaaaatc tcaaaaaaca cctccactta ctgatgcaga
      481 actggaaaag tggctcttct gcaacctggg tggagattct catgccagtg gactcattac
      541 cctctgcttg agtagttcaa atccggctcg accatacatc tggcgtttca acatcttcag
      601 gcgatttaca tgaccttcga caacaccatt actccagcga ctgctgattg cctcacatat
      661 agccgcagca tcagcttcca tccccgcagc cacgcaccga agttctgctg agccgctttc
      721 gtggactcga gtgaacaagc tgcttagctg tgatttattt tgggttttca gaatacggta
      781 gaactcgagt accagttgct gcgctatttt cagctccggt tctttttcac acatcagact
      841 aataaatcgg gaagcataat tttcttcacc cctgattatt aaccagtggc ttacccggga
      901 cactgaagga agtctgaagg aagtctgacg gcagtagtaa ccggtgggat ccagccttta
      961 cgcgatttgg caacagcatc cctgacgatg gtttcactgc ctgtaccccc ctggccacca
     1021 tttcccgcca taccctgctg gcgttataat taccactttc tcgttgctct tttaaccact
     1081 ccctccatgg gtccagaagc cctcgctttg gaggccgggt tgacatttca gggaatgttc
     1141 ctgaacgccc cctgcgactg actgtcacac gcgacaggcc tgtaatacgg gatatttccc
     1201 tgaaactaca tcccttgtta tgcagggcca taacctctgt ccaaagctga tgccgttttt
     1261 tgcgggtttg ctgtttaagg cgttccggac gacggagcga tactgcaggc acagatggtt
     1321 ttgccccggg ttattccttt ggtgacaact aactggtaac aagacgtatc agagtcatat
     1381 gtctgtacat cattcgttca ggcgcatcgc caatattgtt tagcagaagc catcgttcag
     1441 ctacctgtct ggcctgaggt gcaccttcac gtgctgctgt cgcattgatt ccgcagcgat
     1501 cacgcgagac aacctgtatt tccggatatt ttctgaacca ggtcgccagc gtatgctgat
     1561 cgcgaccgtg caacaggacg aggggacggt gagtatcaag attgactatt aacataccgc
     1621 agcagtggcc ccgatgccac gccactcatc aataccgaca tggggcgagc ctgacagttt
     1681 cgtctccagg gtattataag cctgcgaaga agagtatctg cactgcaacg gagacccact
     1741 gtcgttgctg cccgttttcc agcctcacct ccggctatta atcccatctg acgctgtaaa
     1801 ttttgtaacg actgtgaaga ctgctggtgt gaactggcta agggagcaag cgacccggca
     1861 aaaattttac gtgaacaagc cgggttacgg cagtaccagt ggcggacgga aaatacaagc
     1921 catagcgtct ggcctgtgca gggtagatgc tgtatccggc gccggggaca actatgaaca
     1981 gaatcgctac gcttaaagca ttcaggacat tgcgctgttt tgcgttttcc gcggagatgg
     2041 agggttatac cgtcagatct aataatttgt cgactgcatt accatccgca aggtaactgt
     2101 agaagtgtct taagagagtg cattgcgttg cccataactg aaaacagctt agatccttgt
     2161 tcaccagaaa gtttcatcca ttttcttcca cccaggttgc ggaagagcta cttttcatcc
     2221 gtcgttgaca aataatgatg agatagtcac agagcagcgt ttgggaagcg cacaatcaca
     2281 atattgtacg gttgcattat cgcggtagag tataccgcat gctaaaaata agttaagtaa
     2341 gcgtctgaat gatattacat actatatgta tggaaccaaa atacattttt gattaattac
     2401 ttgctttttc aggacggaga cgtatatctc atcctggata caactccaca gagaggagaa
     2461 ttgtttttat ccggaacaaa tctaggacca cgcaggaaac agagtaatac cgcaaagttc
     2521 tagtgattag ctggacaccg ctgcttatta atgagagcaa tatttctgta acaaagataa
     2581 cacattctga gtcagaataa tttcatcatc aggtataacc agcaggcaag cttgcgaacg
     2641 gagcgtgcac acagcccagc ttgcagcgaa cgacctgtac caaaatgaga taccaacagc
     2701 gtgagctatg agaaagcgct acgcttcccg aagggagaaa ggcggagagg tacgcacaag
     2761 ggagcttcca gagggaaagg gagtaccagc tggcgaattt gcactccaac aaataattcc
     2821 cccttttcta ctccactact tttgaccata cgtctggcgc ttaattcgtt gattgttggc
     2881 cttgcaagcg ccgttagacc tgattcacca tacccaggca gtcttggaaa cggaaagctt
     2941 gcgcgttaag cgcacgtttt aactaattca aatacataat gaattgattc aggaataaat
     3001 tgtgatgctc atcacaacaa aagaaggtaa caataataaa atatttatta tttcttatta
     3061 ttatcactgg gaaattataa gaaatgtccg atttttttga atcttacaaa tgacaatgac
     3121 aataaaagcc ggttgttgca tgcattttca ttctttatca ccaataaaaa aataaatttt
     3181 tatttaacat taccccatta aaagatatag acataaaatg aagttttaaa atagtatttc
     3241 agcgagaaag tcgttaaaaa cttaaggtgt tatgcacatc gcacaagtga ttatgaacag
     3301 aatggcattt aaacaaaatg ctgtagtatc tcaattacat ttacaataaa aatagtcatc
     3361 cgaggacaat cgatgaggaa gtcgggtact ccgtggtaaa cataatttat gaatactgaa
     3421 tgaggcaacc tatacttgtc atatccggat aaaatcaccc tctggtaata ctcttaagta
     3481 aatacccagg gtaacaatgt tataaaatta gatgggttat gtttattttt tataagtaaa
     3541 aatcacgagt ataattaatg gtggcagtaa taaaaacata taaaaacaat accgcatatg
     3601 tgaaaaaaat tgcaggagcg gtaattttgt aagcaaacga aaagtatttc actatgtaac
     3661 agacatcaca aaaatacatt aatgcagtca ctatattttc cgcccgggtg ataacgtctg
     3721 gttatcatta gtacttaaac atcatggaga gtccgcagtg aataaagttt attctcttaa
     3781 atattgccct gtcaccgggg gacttattgt tgtctctgaa cttgccagca gggtaataaa
     3841 aaagacatgc cgaagattaa cgcatattct tctggctggc attccagcag tctatctgta
     3901 ttaccctcag atatcccagg cgggcattgt ccgttccgat attgcctatc agatttatcg
     3961 tgatttcgct gaaaacaaag ggctttttgt acctggtgcc acagatattc cggtatatga
     4021 taaggacgga aaacttgtgg ggagactgga taaagcccca atggccgatt tcagcagtgt
     4081 gagctcaaat ggcgttgcta cgcttgtatc acctcagtat atcgtcagcg taaagcataa
     4141 cggaggatat cagagtgtga gctttggtaa tgggaaaaat acatattccc ttgttgaccg
     4201 taataaccac tcttctgttg acttccatgc tccacgtctg aataaactgg ttacagaagt
     4261 tattccctca gcgataacat cagaaggaac caaagccaat gcttataaag acactgaacg
     4321 ttacaccgct ttttatcggg tgggtagtgg tacgcagtac actaaggaca aggacggaaa
     4381 tttagttaag gttgccggcg gatatgcttt taaaacagga ggaaccacag gagttcctct
     4441 gatatctgat gcaacaatag tctctaatcc cgggcaaacc tataatcctg taaacggacc
     4501 tttacctgac tatggtgccc ctggggacag tggttctcct ttgtttgctt atgatgaaca
     4561 acaaaaaaaa tgggttattg ttgctgtatt aagagcatat gcaggtatta atggtgctac
     4621 gaactggtgg aatgtcatac caacagatta tctgaaccag gttatgcagg acgatttcga
     4681 tgcccccgtg gactttgttt ccggactgcc ccccctgaac tggacatacg acaaaacatc
     4741 aggcacaggc accctgagcc agggcagtaa aaactggacc atgcacgggc agaaagacaa
     4801 tgatctcaat gccggtaaaa atctggtatt cagcgggcag aacggtgcaa ttgtcctgaa
     4861 agacagtgtg actcaggggg ccggttatct cgaatttaaa gacagttaca ccgtatctgc
     4921 tgaatccgga aaaacctgga cgggtgccgg cattattact gacaagggga cgaatgtgac
     4981 ctggaaggtc aacggggttg ccggtgacaa cctgcataaa ttgggggaag gaaccctgac
     5041 cataaacgga acaggtgtaa acccgggagg actgaaaacg ggagacggta ccgttgtact
     5101 taaccagcag gcagacactg caggtaatgt tcaggccttc agttccgtga acctcgccag
     5161 cggacgaccg accgtggtgc tcggagatgc ccgtcaggtc aatccggata acatttcatg
     5221 gggataccgg ggaggtaagc ttgaccttaa tggtaatgcc gttaccttca cccgactgca
     5281 ggctgccgat tacggggcgg tgattacaaa taatgcacag caaaaatccc ggcttttact
     5341 ggatcttaag gctcaggata caaatgtcag tgttccgatt ggcagtatat ccccctttgg
     5401 cggtaccggc acaccgggaa acctatacag catgatactc aacggccaga cccgcttcta
     5461 tattctgaaa tctgccagct atggtaacac cctgtggggg aacagcctga atgacccggc
     5521 tcagtgggaa tttgttggca cggacaaaaa caaagcagtt cagacagtaa aagaccggat
     5581 cctggccggg cgggcaaaac aacccgttat ctttcatggt cagctgaccg ggaatatgga
     5641 tgtcaccatt ccacagctgc cggggggaag aaaggtcatc cttgatggta gcgtgaacct
     5701 gccggaaggt accctgagtg aggacagtgg caccctgata ttccaggggc atccggttat
     5761 ccacgcctcc gtcagtggca gtgcgccggt cagcctgaac cagaaagact gggaaaaccg
     5821 ccagttcata atgaaaacac tgtcgctgaa agatgctgac ttccatcttt cacgtaacgc
     5881 ctcgctgaac agtgacatta agtcggataa cagccatatc acactgggaa gtgacagggt
     5941 atttgtggat aaaaatgacg gaacaggaaa ttatgtcatt ctggaggaag gtacctctgt
     6001 cccggacacc gtgaatgaca ggagccagta tgaagggaat attacgctgg accataactc
     6061 aaccctggat atcggcagcc ggttcaccgg agggattgaa gcttatgaca gtgccgtcag
     6121 tatcacctct ccggacgtcc tgttaacagc cccgggtgct tttgccggca gttcactgac
     6181 agtgcatgat ggcggtcatc ttacagcact gaacggtctt ttcagcgacg ggcatattca
     6241 ggccggtaag aacagcaaaa tcaccctgag cggtacaccg gttaaagata cggctaacca
     6301 gtatgcccct gctgtatatc tgacggacgg atatgacctg accggcgata acgcaacact
     6361 ggaaattacc cgtggagcac atgcttccgg tgatattcat gcctctgcgg catcaacagt
     6421 taccatcggg tctgacacgc cggcagaact ggcttctgcg gaaacgactg catcggcgtt
     6481 tgccggcagt cttcttgagg gctataacgc agcattcaat ggtgccataa ccggtggcag
     6541 ggctgatgtc agtatgcata atgcactgtg gactctgggt ggggactccg ccatccacac
     6601 tcttaccgtc agaaacagcc gtatcagttc tgaaggagac cgtactttcc gtaccctgac
     6661 ggtgaataaa ctggatgcaa caggcagtga ttttgttctg cgtacggacc tgaaaaatgc
     6721 cgataaaatt aatgtgactg aaaaagccac gggttcagat aacagcctga acgtcagctt
     6781 tatgaaggat ccggctcagg gacagtccct gaatattcct ctggtcacgg caccggcggg
     6841 aacttcagca gagatgttta aggccggcac ccggatgata ggtttcagtc gggtgactcc
     6901 aaccctgcat gttgacacca gtggtggcaa tacgaagtgg atactggacg gttttaaagc
     6961 ggaggctgat aaagccgctg ccgcgaaggc tgacagtttc atgaatgccg ggtataaaaa
     7021 cttcatgacg gaagtcaaca atctgaacaa acgtatgggt gacctgcgtg acacaaacgg
     7081 tgatgccgga gcctgggcgc gcatcatgag tggtgccggt tctgcagacg gtggttacag
     7141 tgataattac acccatgttc aggtcggctt tgacaaaaaa catgaactgg acggagtgga
     7201 cctgtttacc ggtgtcacga tgacctatac cgacagcagt gcagacagcc atgcgttcag
     7261 cggcaagacg aaatcggtgg ggggcggtct gtatgcttca gcattgtttg agtccggtgc
     7321 ctatatcgat ttgattggta aatatattca ccatgacaat gattacacag gtaactttgc
     7381 cggtctggga acgaaacact acaacaccca ttcctggtat gccggtgctg aaacgggtta
     7441 ccgctatcac ctgacagagg aaacgttcat tgagccgcag gctgaactgg tttacggcgc
     7501 cgtgtccggg aaaacattcc gctggaaaga cggtgatatg gacctgagca tgaagaacag
     7561 ggacttcagt ccgctgattg gaagaacagg gattgaactg ggcaagacct tcagtggtaa
     7621 ggactggagt gtgacggccc gtgccgggac cagctggcag tttgacctac tgaataatgg
     7681 tgagacggta ctgcgtgatg cgtccgggga gaaacggata aaaggcgaga aagacagccg
     7741 gatgctgttt aatgttggta tgaatgcgca gataaaggac aatatgcgct ttggtctgga
     7801 gtttgagaag tcagcctttg gtaaatataa cgtggataat gcggtaaacg caaatttccg
     7861 gtatatgttc tgatgtgggg gaaaggctgc
//
LOCUS       DAZKHK010000181         7852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKHK010000181 DAZKHK010000000
VERSION     DAZKHK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.182"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..194
                     /gene="kdpF"
                     /locus_tag="ACHOS5_004597"
     CDS             105..194
                     /gene="kdpF"
                     /locus_tag="ACHOS5_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP9779300.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            194..1867
                     /gene="kdpA"
                     /locus_tag="ACHOS5_004598"
     CDS             194..1867
                     /gene="kdpA"
                     /locus_tag="ACHOS5_004598"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP9779301.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFLPYQAVTTIEGSQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDVGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1890..3938
                     /gene="kdpB"
                     /locus_tag="ACHOS5_004599"
     CDS             1890..3938
                     /gene="kdpB"
                     /locus_tag="ACHOS5_004599"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP9779302.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASCVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3947..4519
                     /gene="kdpC"
                     /locus_tag="ACHOS5_004600"
     CDS             3947..4519
                     /gene="kdpC"
                     /locus_tag="ACHOS5_004600"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP9779303.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4512..7196
                     /gene="kdpD"
                     /locus_tag="ACHOS5_004601"
     CDS             4512..7196
                     /gene="kdpD"
                     /locus_tag="ACHOS5_004601"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP9779304.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7193..>7852
                     /gene="kdpE"
                     /locus_tag="ACHOS5_004602"
     CDS             7193..>7852
                     /gene="kdpE"
                     /locus_tag="ACHOS5_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP9779305.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IG"
BASE COUNT         1615 a         2077 c         2324 g         1836 t
ORIGIN      
        1 ccatttttat acttttttta caccccgccc gcagattttt gcgaaatctt tgcagccaga
       61 actctaccct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      121 tagccggcgt gttgctggtg tttttattac tgggttatct ggtttatgcc ctgatcaatg
      181 cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt tactggtatt
      241 aatggtgctg gcgcgtccct taggtagcgg gctggcgcgg ctgattaatg atatacctct
      301 tcctggtacg acgggcgttg aacgcgtact ctttagcgcg cttggcgtct ctgaccgcga
      361 gatgaactgg aagcagtatc tctcagccat tcttggcctg aatatactgg ggctggcggt
      421 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
      481 gctgtcgtgg gatctggcgc tcaataccgc cgtcagcttt gtcactaaca ccaactggca
      541 atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcgggct taacggtgca
      601 aaactttctt tctgccgcca gcgggattgc ggtgattttt gcgcttattc gcgcgtttac
      661 ccgccagagc atgagcacgc tcggtaatgc ctgggtcgat ctgctacgca tcacgttatg
      721 ggtgttagcc cctgtggcat tgttgatcgc actgtttttt atacaacaag gtgcgctgca
      781 aaactttttg ccttatcagg ctgtgacgac cattgaagga tcgcaacagc tgttaccaat
      841 ggggccagta gcttctcagg aagcgatcaa aatgctcgga actaacggcg gtggcttttt
      901 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcaaat
      961 gctggcgatt ttcttgatcc caacagcgct gtgctttgcc tttggtgaag tggcgggcga
     1021 tcgtcgccag gggcgcatgt tgctgtgggc gatgtcggtg atttttgtca tctgcgtagg
     1081 cgtagtgatg tgggcagaag ttcagggtaa tccgcatctg ctggcgctcg gcgcggacag
     1141 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1201 ggtcgtgact acggcggctt cctgcggcgc ggtgattgcg atgcatgatt cgtttaccgc
     1261 tcttggtggc atggtgccga tgtggttgat gcaaattggt gaagtggtat tcggcggcgt
     1321 cggttccggc ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1381 gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg agatgaaact
     1441 gactgcactg gcgattctgg tcaccccgac gctggtgctg atgggcgcgg cgctggcaat
     1501 gatgaccgac gtcggacgta gcgccatgct caaccctggc ccgcatggtt ttagcgaagt
     1561 gctgtacgcc gtgtcatccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     1621 caactctcca ttctggaact gtttactggc gttgtgcatg tttgtcgggc gcttcggggt
     1681 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     1741 ctccggcaca ttgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     1801 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggagtatct
     1861 ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     1921 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     1981 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2041 tcgcgatggc aagctgtgtg atgcctggca atgcgctgtt tagcgcggca attagcggct
     2101 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gttggcagaa ggccgcagta
     2161 aagcgcaggc caacagtctg aaaggggtga aaaaaaccgc ttttgctcgc aagctgcgcg
     2221 aaccgaaata tggcgctgcg gcagacaaag ttcctgccga tcaactgcgt aaaggcgata
     2281 tcgtactggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg
     2341 catcggtcga tgaaagcgcc atcaccgggg aatcggctcc ggtgatccgt gaatccggcg
     2401 gtgattttgc ctccgtcact ggcggcacgc gtattctttc tgactggctg gtgattgagt
     2461 gcagcgttaa ccccggcgag acatttctgg atcggatgat cgcgatggtg gaaggtgcgc
     2521 agcgacgtaa aacgccgaac gagattgccc tgaccattct cctgattgcc ctgactatcg
     2581 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgcagtca
     2641 gcgtaacggt actggtggca ctgctggtct gtctgatccc aaccactatt ggcggcctgt
     2701 tgtcagcgat tggcgtcgcc ggaatgagcc ggatgctggg cgcgaatgtt attgccacca
     2761 gcgggcgagc agttgaagca gcgggtgatg ttgacgttct gctgctggat aaaaccggca
     2821 ccatcaccct cggtaaccgt caggcgtcag agtttatccc cgcacaaggc gtggaggaaa
     2881 aagcgctggc cgacgccgca caactggctt cgctggctga tgaaacgccg gaaggccgca
     2941 gtattgtgat cctcgccaag cagcgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3001 atgccacctt tgtacctttt actgcacaaa gccggatgag cgggatcaac atcgacaacc
     3061 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tgttgaggct aacggcggtc
     3121 acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaggga gccacgccgc
     3181 tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag
     3241 gcggtattaa agagcgcttc gcccagctgc gtaaaatggg cattaaaacg gtgatgatta
     3301 ccggcgataa ccgcctgact gccgccgcga tagctgcgga agcgggtgtc gatgattttc
     3361 tcgccgaagc aacaccggag gccaagctgg cactgattcg tcagtatcag gcggaaggcc
     3421 gtttagtggc gatgaccggc gatggcacca acgatgctcc ggcgctggca caggccgatg
     3481 ttgcggtggc gatgaactcc ggcacccagg cggcgaaaga agcgggcaat atggtggatc
     3541 tcgactccaa cccgaccaag ttgatcgagg tagtgcacat tggcaaacag atgctgatga
     3601 cccgtggctc gttgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     3661 ttccagcggc gttcgcggca acgtatccgc agttaaatgc gctgaacatc atgcgcctgc
     3721 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     3781 tgattcccct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     3841 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     3901 tcattgattt actgctaacc gtttgcggtc tggtgtgagg tttaccatga gtggattacg
     3961 tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt acccgctgct
     4021 gaccaccgca ctggggcaat ggtggtttcc ctggcaggcc aacggttcat tgattcgtga
     4081 aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca acggctattt
     4141 tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt ctggcgggag
     4201 caacctggcg gtcagtaacc cggagctgga taaacagata gccgcacgcg ttgctgcatt
     4261 acgggctgct aacccggatg ccagcacgaa cgttccggtt gaactggtga cggcgtcagc
     4321 aagcgggctg gacaataaca tcaccccgca agcggcggcc tggcagatcc cacgcgtggc
     4381 gaaagcgcgt aatctcagcg ttgaacagct cacgcaactg atcgcaaaat acagccaaca
     4441 accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca atctggcact
     4501 ggataagctt gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaag
     4561 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcgg tgcctgcgca ggcgtcggga
     4621 agacctgggc gatgctggcg gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     4681 ttggcgtggt agaaacccac ggtcggaaag ataccgccgc aatgctgaaa ggtctggctg
     4741 ttctgccgtt aaaacgccag gcgtaccgtg ggcggcacat cagcgagttt gatctcgatg
     4801 ccgccctcgc ccgccgtccg gcactgatct taatggacga actggcgcac agtaatgcgc
     4861 caggttctcg tcatcctaaa cgctggcagg acatcgaaga gctgctggaa gctggcattg
     4921 atgttttcac taccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     4981 tcaccggaat tcaggtacgg gagaccgtgc ccgatccttt ttttgatgcc gccgacgacg
     5041 tagtactggt ggacttgccc ccggacgatc tgcgccagcg actgaaagaa ggcaaagtct
     5101 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggt aatctgatcg
     5161 ccctgcgcga actggcactg cgccgtactg ccgatcgcgt agatgagcaa atgcgcgcct
     5221 ggcgggggca tcctggcgaa gagaaagtct ggcacacacg cgacgcgatc ctattatgca
     5281 tcgggcataa caccggcagt gagaaactgg tccgcgcggc ggcgcggttg gcctcgcgcc
     5341 tgggcagtgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaga
     5401 aaaaacgtcg ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg
     5461 caacactttc tgatccagcg gaagaaaaag cggtagtgcg ttatgcccgt gaacataatc
     5521 tcggcaagat tattctcggt cgcccggctt cgcgccgctg gtggcgtcgg gaaacatttg
     5581 ctgaccggct ggcacgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     5641 cacccgcccg cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg
     5701 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcacc ttaattgcca
     5761 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatctg cttggcgtcg
     5821 tggtggtggc actattttac ggacgctggc cttcggtggt tgccaccgtc attaatgtgg
     5881 tgagtttcga tctctttttt atcgccccac gcggcacgct cgccgtctct gatgtgcaat
     5941 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgctg
     6001 gcgtgcgtta tcaggcgcgg gtagcccgtt accgcgaaca acgcacacgg cacttatatg
     6061 aaatgtccaa agctctggct gtgggccgta gttcgcagga tatcgccgcc accagcgaac
     6121 agtttattgc ctccacgttt catgcccgta gccaggtgtt gctgcctgat gacaacggta
     6181 aattgcagcc tttaacacat ccgcaaggaa tgacgccgtg ggacgatgcc atcgcgcagt
     6241 ggagttttga taaaggcctg cctgcgggcg cgggcaccga cacgttaccc ggtgtaccgt
     6301 accagatttt gccgctaaaa agcggcgaga aaacctacgg actggtggtg gtggaaccgg
     6361 ggaatctgcg ccagttgatg atcccggaac agcagcgcct gctggagacg tttacgctgt
     6421 tagtcgccaa tgcccttgag cgactgacac tgaccgccag cgaagaacag gcgcggatgg
     6481 caagcgaacg tgaacagatc cgcaacgccc tgctggcggc gctttcgcat gatttacgca
     6541 cgccgcttac ggtgctgttc ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag
     6601 gatcgcccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac accacccgac
     6661 tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag
     6721 agtggttaac gctggaagaa gtggtcggca gcgcgctgca aatgctggaa ccgggtttat
     6781 cgtcgcccat caatctttct ctgccagagc cgctgacctt aatccacgtt gacgggccac
     6841 tctttgaacg ggtgctgatt aacctgctag agaacgcggt gaaatatgcg ggggcgcagg
     6901 ccgaaattgg tatcaatgcc cacgttgagg gcgaaaatct acaactggat gtctgggata
     6961 acggccccgg tcttccacca ggccaggagc agacgatatt tgataagttt gctcgcggga
     7021 ataaagagtc ggcggtaccg ggtgtagggc ttgggttggc aatttgtcgg gcgatagtgg
     7081 atgtacacgg cggcaccatt accgcattca accgaccgga aggtggtgcc tgttttcgtg
     7141 ttacacttcc ccagcaaaca gcccctgaac ttgaagaatt tcatgaggat atgtgacaaa
     7201 cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg
     7261 cgacggtatg cgcgtctttg aggccgaaac gctgcaacgc ggcttgctgg aagcggcaac
     7321 ccgtaagcca gatttgatta ttctcgatct cggcctgccc gacggtgatg ggattgagtt
     7381 tatccgcgac ctgcgccagt ggagcgcggt gccggtgatt gtgctttccg cacgcagcga
     7441 agagagcgat aaaatcgccg cgctggatgc cggagcggat gattatctga gtaagccgtt
     7501 tggcattggc gaattgcagg cccgtctgcg cgtcgcatta cgccgccact ccgccaccac
     7561 cacgcccgat ccgctggtga aattttctga tgttaccgtc gatttagccg cccgcgttat
     7621 ccaccgcggc gaggaagagg tgcatctcac accgattgag ttccgcctgc tggccgtgct
     7681 gctcaacaac gccggaaaag tgctcaccca gcgccagtta ctcaatcagg tgtggggacc
     7741 aaacgcggtc gaacacagcc actatttgcg tatttatatg gggcatctgc gacaaaaact
     7801 ggagcaagat cccgcccgtc cccgccattt tattactgaa accggtattg gg
//
LOCUS       DAZKHK010000182         7847 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKHK010000182 DAZKHK010000000
VERSION     DAZKHK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.183"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /locus_tag="ACHOS5_004603"
     CDS             <1..216
                     /locus_tag="ACHOS5_004603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779306.1"
                     /translation="QASHQANSAGCGVNALSGLQKKCRKSLKTAPDTKTVFKGSLADA
                     ENTTSPQQVNPGTHIRYEMCVVVSTAK"
     gene            245..3046
                     /gene="sucA"
                     /locus_tag="ACHOS5_004604"
     CDS             245..3046
                     /gene="sucA"
                     /locus_tag="ACHOS5_004604"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP9779307.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQEKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            3061..4278
                     /gene="odhB"
                     /locus_tag="ACHOS5_004605"
     CDS             3061..4278
                     /gene="odhB"
                     /locus_tag="ACHOS5_004605"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9779308.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPTLAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            4372..5538
                     /gene="sucC"
                     /locus_tag="ACHOS5_004606"
     CDS             4372..5538
                     /gene="sucC"
                     /locus_tag="ACHOS5_004606"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9779309.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            5538..6407
                     /gene="sucD"
                     /locus_tag="ACHOS5_004607"
     CDS             5538..6407
                     /gene="sucD"
                     /locus_tag="ACHOS5_004607"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9779310.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            6647..7768
                     /locus_tag="ACHOS5_004608"
     CDS             6647..7768
                     /locus_tag="ACHOS5_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9779311.1"
                     /translation="MGVNSDVYAADVNIDILSATVKDKRIEGVSVTLQRNGAQSVSGT
                     TNASGSVNLGSTFADDQDALLIVKKEGYSNLVVKCSCAGMTYAISPAMTSLDGMRVVL
                     SWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGSETVTISKKHFGESYIY
                     AVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIWTVFKLNPNGEFE
                     DINSVTSANFNDTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQLARKYNREGEAVY
                     KTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWANRKAFSLASGVNA
                     ATTRANSYYNIAKIYETSGQNAEALQHYQLALHDAILQQ"
BASE COUNT         1965 a         1985 c         2136 g         1761 t
ORIGIN      
        1 caagcgtcgc atcaggcaaa cagcgccgga tgcggcgtga atgccttatc cggcctacaa
       61 aagaaatgca ggaaatcttt aaaaactgcc cctgacacta agacagtttt taaaggttcc
      121 ttagcggacg cagaaaacac gacaagtccg caacaagtga accccggcac gcacatcaga
      181 tatgaaatgt gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat
      241 cacgatgcag aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa
      301 ccagagctgg atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc
      361 taactggcgt tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca
      421 ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac
      481 gatctccgac cctgacacca atgtgaagca ggttaaagtc ctgcagctca ttaacgcata
      541 ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagagaa
      601 agtggccgat ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggagacctt
      661 caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc
      721 cctcaagcaa acctactgcg gcccgattgg tgccgagtat atgcacatta ccagcaccga
      781 agaaaaacgc tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga
      841 gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt accttggcgc
      901 aaaattccct ggcgcaaaac gcttctcgct ggaaggtggt gacgcgttaa tcccgatgct
      961 taaagagatg atccgccacg ctggcaacag cggcacccgt gaagtggttc tcgggatggc
     1021 gcaccgtggt cgtctgaacg ttctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt
     1081 cgacgagttt gccggtaaac ataaagaaca cctcggcacg ggcgacgtga aataccacat
     1141 gggcttctcg tctgacttcc agaccgatgg cggcctggtt cacctggcgc tggcgtttaa
     1201 cccgtctcac cttgagattg taagcccggt cgttatcggt tctgttcgtg cccgtctgga
     1261 cagactcgac gagccgagca gcaacaaagt gctgccaatc accattcatg gtgacgccgc
     1321 agtgaccggg cagggcgtgg ttcaggaaac cctgaacatg tcgaaagcgc gtggttatga
     1381 agttggcggt acggtacgta tcgttatcaa caaccaggtt ggcttcacca cctctaaccc
     1441 gctggatgcc cgttcgacgc cgtactgtac tgatatcggt aagatggttc aggcaccgat
     1501 tttccacgtt aacgcggatg atccggaagc cgttgccttt gttacccgtc tggcgctcga
     1561 tttccgtaac acctttaaac gtgatgtctt catcgacctg gtatgctacc gccgtcacgg
     1621 ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa
     1681 acatccgacg ccgcgcaaaa tctacgctga caagctggag caggaaaaag tcgcgacgct
     1741 ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt
     1801 tgtagcagag tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca
     1861 cgaatgggac gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg aactggctaa
     1921 acgcatcagc acggtgccgg aagcggttga aatgcagtct cgcgttgcca agatttatgg
     1981 cgatcgccag gcgatggcag ccggtgagaa actgttcgac tggggcggcg cggaaaacct
     2041 cgcttacgcc acgttggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg
     2101 tcgcggtacc ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta
     2161 cacgccgctg caacatatcc ataacggcca gggcgcgttc cgcgtctggg actctgtact
     2221 gtctgaagaa gccgtactgg cgtttgaata cggttatgcc accgcagaac cacgcaccct
     2281 gaccatctgg gaagcacagt tcggtgactt cgccaacggt gcacaggtgg ttatcgacca
     2341 gttcatctcc tctggcgaac agaaatgggg ccggatgtgt ggcctggtga tgttgctgcc
     2401 gcacggttac gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca
     2461 actttgcgct gagcaaaaca tgcaggtttg cgtaccttct accccggcac aggtttacca
     2521 catgctgcgt cgtcaggcgt tgcgcgggat gcgtcgtcca ctggtcgtga tgtcgccgaa
     2581 atccctgctg cgtcatccgc tggcggtatc cagcctcgaa gaactggcga acggcacctt
     2641 cctgccagcc atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat
     2701 gtgttctggt aaggtttatt acgacctgct ggaacaacgt cgtaagaaca atcaacacga
     2761 tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt
     2821 gttgcagcag tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca
     2881 gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct
     2941 gcgttatgca ggccgcccag cctccgcctc tccggcggta gggtatatgt ccgttcacca
     3001 gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca
     3061 atgagtagcg tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc
     3121 gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc
     3181 gaaactgaca aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt
     3241 ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc
     3301 aacagcgcgg gtaaagaaac cagcgcgaaa tctgaagaga aagcgtccac tccggcgcaa
     3361 cgccagcagg cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc
     3421 ctgctggctg aacataacct cgacgccagc gccattaaag gcaccggtgt gggtggtcgt
     3481 ctgacccgtg aagatgtgga aaaacatcta gcgaaagccc cggcgaaaga gtctgctccg
     3541 gcagcggctg ctccggcggc gcaaccgacc ctggctgcac gtagcgaaaa acgtgtgccg
     3601 atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc
     3661 atgctgacca cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac
     3721 ggtgaagcgt ttgaaaaacg ccacggcatt cgtctgggct ttatgtcctt ctacgtgaaa
     3781 gcggtggttg aagccctgaa acgttacccg gaagtgaatg cgtctatcga cggcgatgat
     3841 gtggtctacc acaactattt cgacgtcagt atggcggttt ctacgccgcg cggcctggtg
     3901 acgccggttc tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa
     3961 gagctggctg ttaaaggccg tgacggcaag ctgacggttg aagatctgac cggtggtaac
     4021 ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt caacgcctat catcaaccca
     4081 ccgcagagcg caattctggg tatgcacgct atcaaagatc gtccgatggc agtgaatggc
     4141 caggttgaga tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tttgatcgat
     4201 ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt
     4261 ctgctgctgg acgtttagta gtttaagctt cacctgcact gtagaccgac gtggataggg
     4321 tgtcggtcta cggtttaaaa gataacgatt actgaaggat ggacagaaca catgaactta
     4381 catgaatatc aggcaaaaca actttttgcc cgctatggct taccagcacc ggtgggttat
     4441 gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa aaatcggtgc cggtccgtgg
     4501 gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag cgggcggtgt gaaagttgta
     4561 aacagcaaag aagacatccg tgcttttgca gaaaactggc tgggcaagcg tctggtaacg
     4621 tatcaaacag atgccaatgg ccaaccggtt aaccagattc tggttgaagc ggcgaccgat
     4681 atcgctaaag agctgtatct cggtgccgtt gttgaccgta gttcccgtcg cgtggtcttt
     4741 atggcctcca ccgaaggcgg cgtggaaatt gaaaaagtgg cggaagaaac tccgcacctg
     4801 atccacaaag ttgcgcttga tccgctgact ggcccgatgc cgtatcaggg acgcgaactg
     4861 gcgtttaaac tgggtctgga aggtaaactg gttcagcagt tcaccaaaat cttcatgggc
     4921 ctggcgacca ttttcctgga gcgcgacctt gcgcttatcg aaatcaaccc gctggttatt
     4981 accaaacagg gcgatctgat ttgcctcgac ggcaaactgg gcgctgatgg caacgcactg
     5041 ttccgccagc ctgatctgcg tgaaatgcgt gaccagtcgc aggaagatcc gcgtgaagca
     5101 caggctgcac agtgggaact gaactacgtt gcgctggacg gtaacatcgg ttgtatggtt
     5161 aacggcgcag gtctggcgat gggtacgatg gacatcgtta aactgcacgg cggcgaaccg
     5221 gctaacttcc tggacgttgg cggcggcgca accaaagaac gtgtaaccga agcgttcaaa
     5281 atcatcctct ctgacgacaa agtgaaagcc gttctggtta acatcttcgg cggtatcgtt
     5341 cgttgcgacc tgatcgctga cggtatcatc ggcgcggtag cagaagtggg tgttaacgta
     5401 ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg gcgcgaagaa actggctgac
     5461 agcggcctga atattattgc agcaaaaggt ctgacggatg cagctcagca ggttgttgcc
     5521 gcagtggagg ggaaataatg tccattttaa ttgataaaaa caccaaggtt atctgccagg
     5581 gctttaccgg tagccagggg actttccact cagaacaggc cattgcatac ggcactaaaa
     5641 tggttggcgg cgtaacccca ggtaaaggcg gtactaccca cctcggcctg ccagtgttca
     5701 acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc ctctgttatc tacgtaccag
     5761 caccgttctg caaagactcc attctggaag ctatcgatgc aggcatcaaa ctgattatca
     5821 ccatcactga aggcatcccg acgctggata tgctgacggt gaaagtgaaa ctggatgaag
     5881 caggcgttcg tatgatcggc ccgaactgcc caggcgttat caccccgggg gaatgcaaaa
     5941 tcggtatcca gccgggtcac attcacaaac cgggtaaagt gggtatcgtt tcccgttccg
     6001 gtacgctgac ctatgaagcg gttaaacaga ccacggatta cggtttcggt cagtcgacct
     6061 gcgttggtat cggcggcgac ccgatccctg gttctaactt tatcgacatc ctcgaaatgt
     6121 tcgaaaaaga tccgcagacc gaagcgatcg tgatgatcgg tgagatcggc ggtagcgctg
     6181 aagaagaagc agcggcgtac atcaaggagc acgttaccaa gccagttgtg ggttacatcg
     6241 ctggtgtgac tgcgccgaaa ggcaaacgta tgggtcacgc aggtgcgatc attgccggtg
     6301 gtaaagggac tgcggatgag aaattcgctg ctctggaagc cgcaggcgtg aaaaccgttc
     6361 gcagcctggc ggatatcggt gaagcactga aaactgttct gaaataaata tctgtaataa
     6421 gaaatagccc tcgccgcttc cctctacagg aggggcgaag ggctgtcggt ttcgacatgg
     6481 ttggccatct tatgatggcc ttttttattc gggcactaat ctgaaaagtt tgcccactgc
     6541 ttttctccca tcttctgcgc aataatgccc ggcaaaatat cccctctccg ttctgtaaaa
     6601 gcatttatac aagaagaaaa ttgttctgcc ggttgctctg gccctgatgg gtgtaaattc
     6661 ggacgtttat gcggccgatg taaatatcga tattttaagt gcgacagtga aagataaacg
     6721 cattgaggga gtatcggtga cgctgcaacg caatggcgca caatctgttt ctggaaccac
     6781 aaatgcatcg ggcagcgtta atctgggttc tacatttgct gatgatcaag atgctttatt
     6841 gatcgtcaaa aaagagggct attctaatct ggtggtcaaa tgttcgtgtg ctggcatgac
     6901 ttatgcgatt agtccggcaa tgactagtct ggatggcatg cgtgtagttt tgagctgggg
     6961 cgaaaagcct tttgatcttg attcacattt gatcttccct ggcggacata tctattttga
     7021 tagtaaagag gggactgatg ctaatctgga tgttgatgat acggatagct atggatcaga
     7081 aactgtcact atcagcaaaa agcattttgg cgaaagttac atctatgccg tgcaggacta
     7141 tagcaacaaa gggttgccca acagtaatta tctctctgcc agtaaggcaa aagtgtttgt
     7201 ttatgtaggg agctccctgg tgcgtagtta tagtgtccct gctggaaaac gagggaacat
     7261 ctggaccgtg tttaaactca atccaaacgg cgagtttgaa gatattaact ctgttaccag
     7321 cgctaatttt aacgacacca cattagacgt cagggattta gccactgtga taatgcctgc
     7381 gacggactct tcagctccgg cttcgcctgc gatgcaaaac agtggagata ctcaactggc
     7441 aagaaaatat aatcgcgaag gtgaagctgt ttataaaacc ggtcaacttg agcaagccat
     7501 tcaactcttc caacaggcaa cagaattaga cggcaattac ggacaggcat ttagtaacct
     7561 gggtctggcg tatcagaaaa atggcaacat agcggaagca atttgggcaa atcgtaaagc
     7621 gttctcgtta gcgagtgggg taaatgcggc gacaacgcgc gccaactcct attataacat
     7681 tgccaaaatt tatgaaacct cgggccagaa tgctgaagca ttgcagcatt atcaactcgc
     7741 tcttcacgat gccattttac aacaatgaaa gtggaatcat atttttaaat tttatattct
     7801 tgcttgtttt tctggtgaag ttatccatca ttcactgttc tattatc
//
LOCUS       DAZKHK010000183         7779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKHK010000183 DAZKHK010000000
VERSION     DAZKHK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.184"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..898
                     /locus_tag="ACHOS5_004609"
     CDS             <1..898
                     /locus_tag="ACHOS5_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFP9779312.1"
                     /translation="SIQVHFLNLPSYRRFPNDDEFKRELKVRDLYNFRSRSYWLRRLE
                     NDKRRERVEEFTIEHIMPQNENLSAKWREELGSDWQRIHKELLHTLGNLTLTRYNSRY
                     SDRPFAEKRDIEDGFKHSPLYLNIGLGQCEKWDEAAIRARADRLADLAVQVWQAPALP
                     EEVLAVYRAQPENKTSYSLSDYPFLADGSHSRVLFDHLRDEVMRLDAGITQEVLKLYI
                     AFKAETNFVDVVPQKSRLRLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLA
                     QLPYIMGLIRQAFEKQMESALV"
     gene            complement(945..1286)
                     /gene="symE"
                     /locus_tag="ACHOS5_004610"
     CDS             complement(945..1286)
                     /gene="symE"
                     /locus_tag="ACHOS5_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFP9779313.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESELMQSLRQVCKLSARKQRQVQEF
                     IGVIAGKQKVA"
     gene            complement(1507..3213)
                     /locus_tag="ACHOS5_004611"
     CDS             complement(1507..3213)
                     /locus_tag="ACHOS5_004611"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9779314.1"
                     /translation="MNVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKLFELP
                     EGWEWVRFGNIYEMEYGNNLPQEKRSNSGEYNVYGSNGVVGTHNEACIKSPCIIIGRK
                     GSAGALNLSNQPACWVTDVAYSTIPPIAMVLEFVFIQFHTLGLDKLGKGIKPGLNRND
                     AYSLVIAIPPRSEQKAIVSKVNELMSLCDQLEQQSLTSLDAHQQLVETLLGTLADSQN
                     AEELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRI
                     AQEKAQLVKEGKIKKQKPLLPISDEEKPFELPNGWEWCRLGELIDSIDAGWSPACSSE
                     PAAPGEWGVLKTTAVQSLEYREYENKALPKNKAPRPQLEVKAGDILITRAGPKNRVGI
                     SCLVENTRENLMISDKIIRFHLISEDISEKYISLCLNYGFTSTYLENSKSGMAESQMN
                     ISQDILKMAPIAIPTTHEQLKITDKINEMMDYFITLKSQIQSAQQTQLHLADALTNAA
                     IN"
     gene            complement(3213..4682)
                     /locus_tag="ACHOS5_004612"
     CDS             complement(3213..4682)
                     /locus_tag="ACHOS5_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9779315.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRIDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            complement(4749..7181)
                     /gene="hsdR"
                     /locus_tag="ACHOS5_004613"
     CDS             complement(4749..7181)
                     /gene="hsdR"
                     /locus_tag="ACHOS5_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFP9779316.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            7459..>7779
                     /locus_tag="ACHOS5_004614"
     CDS             7459..>7779
                     /locus_tag="ACHOS5_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFP9779317.1"
                     /translation="MEAKECKVQDILTENKKFIIPSYQRPYSWTVDNAEQLIDDIYKS
                     SQSEENEYFIGSMICINKGQNQYEVVDGQQRLTTLSIIVSELKKIIPIQGIKDDLQKR
                     VLPID"
BASE COUNT         1861 a         1770 c         1859 g         2289 t
ORIGIN      
        1 aagtattcag gtacattttc tgaatctgcc ttcatatcgt cgtttcccca acgatgatga
       61 atttaaacgg gaattaaaag ttcgtgatct ctataatttc cgtagtcgca gctactggtt
      121 acgacgactg gaaaacgata aacgcagaga gcgcgtggaa gagtttacga ttgagcatat
      181 tatgccgcag aacgaaaatt tgtcggctaa atggcgtgaa gagctgggaa gtgactggca
      241 acgcattcat aaagaactgt tgcatacgtt ggggaatctc acattaacgc gctataactc
      301 ccgctacagt gacagacctt tcgcggaaaa acgcgatatt gaagacggct ttaaacatag
      361 cccgctctat ttgaatatcg gtcttggaca gtgcgaaaaa tgggatgaag cagccattcg
      421 cgcccgtgca gatcgtctgg ccgatctcgc ggttcaggtc tggcaagcgc ctgctcttcc
      481 tgaagaggtt ttagctgttt atcgggcgca gccggagaac aaaaccagtt acagcctgag
      541 tgattatcct tttcttgctg atggttcgca tagccgggtg ttatttgatc atcttcgcga
      601 tgaagttatg cgcctggacg cagggatcac gcaggaagtt ttaaagctct atattgcgtt
      661 taaagctgaa acgaattttg ttgatgttgt accgcaaaaa agccgactgc ggttgtcgct
      721 taatatgcag tttcatgaac tggtcgatcc gaaaggtatt gccaaagatg tgacaaatgt
      781 tgggcgatgg ggcaatggtg atgtggaaat tggtttcagt gacctcgcac aacttcctta
      841 cattatggga ttaattcgtc aggcatttga aaaacagatg gagagcgcgt tggtataacg
      901 tggcgaataa tacccggtag cactggcgct accgggtcaa tgtatcaggc gactttctgt
      961 ttacccgcaa tcaccccaat aaactcctgc acctgccttt gtttacgcgc cgacagcttg
     1021 cacacctggc gcagcgactg catcagttcg ctctcggcgg cggcgggtgg ttgggcggtg
     1081 aggacaatac agccttccat tactttgaca tctaccgcag tgccggtggc aaaaccggcg
     1141 gcttccagcc actgaccttt cagggtgatg gcgggaatac ggctgtaatc cgggtagcga
     1201 ctcgcataac tcacggttaa ttgacggtta tttgccgggg agacttctgc ttcgaacggt
     1261 tgtgcaatag aatgcgtgtc agtcataact gctattccct ataaatagtg attgtgatta
     1321 gcggtgcggg tgtgttggcg cacatccgca ccgcgctaaa tacctggaca tatcatcagt
     1381 aaatacaggg aaagtccagc taaaaattaa tgaaaataag atgaaaatta gcgtgaaata
     1441 taatttttgc tgatgtgaat aaagcggcta ttgagccgct ttattgcttt tttgagggcg
     1501 ggaaaattag tttatcgccg cgttagtcag tgcgtctgca aggtgcagtt gggtttgttg
     1561 ggcagattga atttgactct ttagagttat aaaataatcc atcatttcat ttattttatc
     1621 tgttattttt aattgttcat gtgtagtcgg gatagctata ggtgccattt ttaaaatatc
     1681 ttgtgaaata ttcatctgac tttctgccat gccagatttg gagttttcaa gatatgttga
     1741 agtaaaccca tagtttagac ataaggaaat gtatttctcg gagatatcct ctgatatcaa
     1801 atgaaatctg attattttat ctgaaatcat taaattttct cttgtgtttt caacaaggca
     1861 tgaaatacca acccgatttt taggaccagc tcttgttata agaatatctc ctgcttttac
     1921 ttctagttgt ggccttgggg ctttattttt agggagcgcc ttgttttcgt actctctata
     1981 ttcaagagat tgaactgcgg tcgtttttaa caccccccat tctcctggag cggctggttc
     2041 agaagaacat gcaggactcc acccagcatc aatggaatca ataagctctc cgagcctgca
     2101 ccattcccat ccattaggca gttcaaacgg tttttcctca tcgctaattg gcagcaacgg
     2161 tttttgtttt tttattttcc cttctttcac cagttgagct ttttcctgcg caattcgttt
     2221 gagcagttca gaggcgggtt cgtcattcgg atcctgcggc acaagtttac ccattacggc
     2281 cagttgcaga atggtctgtt ttaacgcatc cacgctggct tcggtggtaa aaagtgtgtc
     2341 gaaatgctcg ctaatacgcg cccagttttc agccagttcc tcggcgttct ggctgtctgc
     2401 aagtgttccc aacagggttt caaccagttg ctgatgtgcg tccagactgg tcaaggattg
     2461 ctgttccagt tggtcgcata gggacattaa ttcatttact ttcgacacta tggctttctg
     2521 ttcgcttcga ggtggaatag caattactaa actataagca tcgttacgat tcaagcctgg
     2581 cttaattccc ttccctaatt tatcgaggcc caatgtatga aattgtataa agacaaattc
     2641 aagaaccata gcaattgggg gtatagtgct ataagctacg tctgttaccc aacaggctgg
     2701 ctgatttgaa agatttaatg ctcctgcgga accttttctg ccaataataa tacatggtga
     2761 tttaatacat gcttcattgt gtgtacctac aactccattg gagccataaa cattatattc
     2821 accagaatta gatctttttt cttgtggtaa attattaccg tactccattt catagatatt
     2881 accaaatcgc acccactccc atccctccgg caattcaaat agcttctctt cctcgctaat
     2941 ttccggcagt ggtttttgct ttttaatttt cccctgtttc accagctcgg ctttttccgc
     3001 cgcaatacgc ttcagcagct ccgacgccgg ttcatcattc ggatcctgcg gcaccagttt
     3061 accgcgcaca gccagttcca gaatcagctc acgtaatttc ttaatgccat ataaatcaat
     3121 ttttccggaa ctgccgcgcc cggcggtgga acgggtttgc aacgccgagg tccaggtttc
     3181 catatgatca acgatcagct tttccacatt cattagttaa cctccttgcc agacagcgca
     3241 gcgccaagaa tatcgcgcag ttgattacgc agcgtctgga tctccgcctg ctgttttgca
     3301 tactgcgcta acagttcgtc cgggtcatgg ctgacggttt ccgcctgatg tgggttttta
     3361 atatccagat tgaagttacg cgcaatcacg tcatcaatgc tgactttcca cgcttgctca
     3421 ttctcgatgc ggctggcaaa accatcggcc tcgttacccc accagtcaat ctccgcctga
     3481 aactcttcga acttcatcgg cttggtttta ctgtaatttt tcacgcccgc cggatacgga
     3541 tgctcataga accaaatctc tttggttggc tgacctttgg taaaaaacaa caggttggtt
     3601 ttaatgccgg tataggggtt aaacacacca ttcggtaaac gcacgatggt atgcagattg
     3661 cactcttcgg taagcagctt tttgattttg gttttaacgc cttcgccaaa tagcgtgcca
     3721 tccggcaata ccaccgccgc gcgaccgttt ttcgccagta cttccacaat cagttgcaga
     3781 aacagatccg ccgtttcgcg ggtttgcatc tctgccggaa agttcttctc aataccgtct
     3841 tcttccgtgc caccaaacgg cgggttggta acaatgacat ccagttgctc atcccaggag
     3901 gaaagcggtt tgttcagggt gttgtcgtga cgaatttgta caggcacttc aatgccgtgc
     3961 agcagcatat tggtggtcgc cagcaggtgc ggaagctgtt ttttctcgac accgtggatc
     4021 tgttgttgca gcgtctgatg atcggcgacg ctcttcacgt atttgttctt tacatgatcg
     4081 aacgcgcagg caagaaaacc gcccgtaccg caggccgggt ccataatgga ttcgccgagt
     4141 ttcggatcaa tgcgatccac cataaagcga gtgacggcgc gtggagtata gaattcgccc
     4201 gcattgcccg cagattgcag atctttaagg atctgttcgt aaatatcacc aaacagatgg
     4261 cgttcgctgg cgctggtaaa gtcaatttcg ttcagcttgt tgatcacctg gcgcagtagc
     4321 gtaccgtttt tcatgtagtt ataggcatcg ctgaacgcct gctttaccac gtagccgcgt
     4381 gggtttttat cgataggcgc agtgaggttt ttcaacgccg ggaacagatc atcattaacg
     4441 aattccagca gggaatcacc ggtaatgccc tgagcgtttg cggcccaact gcgccataaa
     4501 taacgctgtg ggatcggata ttgatagtta tcctgctcca gttccagcgc ctcttcctgg
     4561 gcatcgaaga ttttcaaaaa cagcagccag gagagctgac cgagacgctg cgcatcgccg
     4621 tccacaccgg catctttgcg cataatatct tgtaatgatt tgattactga actgatagac
     4681 atgttgtatt ccatgaaaaa caaggccttc tgcaacatgt tggttgcaga aggcacaaaa
     4741 gagagttgtt aagcagagcg gggcggtaat tggtagattt cgttttccag ctcgctgagc
     4801 gcctgattat acccgttacg gtcgccaaat ccggttttaa taatctccgg tagggtgccc
     4861 atgctgtcga atggtttaag tttcagcacc tgaatacttt cgatctcctg tacgcccgca
     4921 tcggcgtatt tatccagcag attatcgagc acggcttgcg ctgcttcaga gtattttgtg
     4981 aagtagttcc gcttgcgcac gttctcggcg cgctctttgc gggttaacgg cggctgacca
     5041 tacactacgt ggcaaagcat gtcgaacggg tcgagatctt taccgacttc ttctgccagt
     5101 acttcccaga tgatcccctg ttgttccagc tcgtgaatga tcgcttcttt gcgatcggcg
     5161 tcttgccact tgcgggtaaa gtcatccagc gaggcatatt ctttgagcag tgttttgcgg
     5221 gtgtaatctt taaaagattc ggtaaccagt ttaccgtcgg catcgtaata ctgaacacgc
     5281 tgcgcaataa cacccaccgc cacgccattg acgtgaaact tgcggatttt cttctcgtct
     5341 tcttccggaa gtgggccaac atcatcggta tcggtaacct ggtatggtgg cgcaggcggt
     5401 tcatcaacgc ctgttacctg ttcgtcgtca tgttcgctga tttcttcgag tttctcttca
     5461 aaatcagatt ctggatcggc gatatcctct ggtgtggtat ccatgacttt ttcgggaatg
     5521 ccatcgaaac gctcatcggc aaacagttcg gtggcttttt taaagtcgag gatggtaaac
     5581 cagagtttgc cgtaacgttc gtcgatgcgt gtaccacgac cgataatttg cttgaatttg
     5641 gtcatcgact ggatgttctg gtccagtact accagtttgc aggttttagc atccacacca
     5701 gtggtcatca gttctgaagt ggtcgcgata accgggtacg cttttttcgg gttgatgaag
     5761 ttatccaact gagctttgcc aatttcatca tcgccggtga ttttcatgac gtatttgtcg
     5821 ttctttttca cctgctccgg gttgagattc accagggcgc ggcgcatacg ttctgcatga
     5881 tcgatgtcgt tacagaagac gatggtttta tccatcgggt tggtgcgctt gaggtagtcg
     5941 gtaatggttc tggcaaccag ttctgtgcgt tcgtcgatta ccatcgtgcg atcgaaatct
     6001 ttctggttat agatacgatc gtcgatcact tcgccgttta agtcagtttg ccctttggtt
     6061 gggcgccagc cttgcagatc aacatcaata tcaacacgga caactttata aggggcgagg
     6121 aagccgtctt cgatcccttc ttttagtgag tagacgtaaa ccggatcgcc gaagtaatcc
     6181 gtgctggaga cttcatgcgt ctcttttggc gtggcggtta agccaatttg ggtggcggaa
     6241 ctgaaataat caaggatctc tcgccaggcg ctgtcttcgg atgcgctgcc gcgatggcat
     6301 tcgtcgatca cgatcagatc gaagaaatct ggtgcgactt gtttaaacgc tttttggtct
     6361 tcctccgggc cagttatagc ctgatagagc gcgaggtgaa tttcataagc gggatcaatg
     6421 gtgcgtccgc tgactttggt cattgccgta ccaaatggct ggaaatcatt atttttggtt
     6481 tggtcgacca gaatgttgcg atcggcaagg aacaaaatgc gttttttatt ttttgatttc
     6541 cacaggcgcc agatgatctg gaatgcggtg taggttttcc ctgttccggt cgccatgacc
     6601 agcagaacgc ggttttgccc gttggagacg gcttcaatgg ttttgttgat tgcctgaagc
     6661 tggtaataac gtggcgattt accgctgcca tcgtcgtaat agtcctgagt aatcaccgga
     6721 agctgagctt gtgtgtatcc tttccagagg cagaattttt gccagagttc agcgggggag
     6781 gggaagtcat ccagcgttat ttgcttttca aggcattcac cttcggctgc ggtggcatcg
     6841 cggaagataa agccatcccc gttggtggca aaaacaaaag gaacgtccag caggcgcgca
     6901 tattcaatgc cctgttgcat ccctttgcca atttcatgtt tgttggcttt tgcttcaatc
     6961 acggctaagg gaatgcctgg tttgtgatac agcacgatgt cagcagattt taccgttctg
     7021 cgtgccgcaa ctttaccacg tacgatgact ttaccgtccc ggagtttgac ctcctgtctg
     7081 atttgcgttg tgttgtccca gcctgcattg agaatggctg gcataacgca tttggtaatg
     7141 atgtctgctt ccgtcaggtt acttaggttc agttccgcca tggggtgttc tcgcttttat
     7201 caggacgatt ctgattgtac ataaaatcaa tagcctggct atgacttagg gcggtgtttc
     7261 aacgtgaagt acgtagcaga gatcacattg atatctctgt tatatcttgc ttgaattaat
     7321 ctgggcatat cagagacaga taaacagaag ttacaggaac cgtatcaact ctaacaaaat
     7381 atataaaaat cttatagttg cgtttctttc gagcaagaaa gataatgtta ttgttgattg
     7441 tttgattggg tatatgaaat ggaagctaaa gaatgtaagg tgcaggatat ccttactgaa
     7501 aacaaaaagt ttattattcc ttcttatcag cgtccttaca gttggacggt agataatgca
     7561 gagcaactca ttgatgacat ttataaaagc tctcaatcag aagagaatga atattttatt
     7621 gggagtatga tttgtatcaa taaagggcaa aatcagtatg aagttgtaga tggacaacaa
     7681 cgtcttacaa cgttaagcat tattgtctcc gagctaaaaa aaatcatccc gattcagggg
     7741 ataaaagatg atttgcaaaa aagagttctg cctattgat
//
LOCUS       DAZKHK010000184         7653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKHK010000184 DAZKHK010000000
VERSION     DAZKHK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.185"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            136..471
                     /gene="eutS"
                     /locus_tag="ACHOS5_004615"
     CDS             136..471
                     /gene="eutS"
                     /locus_tag="ACHOS5_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP9779318.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            484..963
                     /gene="eutP"
                     /locus_tag="ACHOS5_004616"
     CDS             484..963
                     /gene="eutP"
                     /locus_tag="ACHOS5_004616"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP9779319.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            938..1639
                     /gene="eutQ"
                     /locus_tag="ACHOS5_004617"
     CDS             938..1639
                     /gene="eutQ"
                     /locus_tag="ACHOS5_004617"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP9779320.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1636..2439
                     /gene="eutT"
                     /locus_tag="ACHOS5_004618"
     CDS             1636..2439
                     /gene="eutT"
                     /locus_tag="ACHOS5_004618"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP9779321.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSSLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2436..3452
                     /gene="pta"
                     /locus_tag="ACHOS5_004619"
     CDS             2436..3452
                     /gene="pta"
                     /locus_tag="ACHOS5_004619"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9779322.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            3491..3784
                     /gene="eutM"
                     /locus_tag="ACHOS5_004620"
     CDS             3491..3784
                     /gene="eutM"
                     /locus_tag="ACHOS5_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP9779323.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3891..4178
                     /gene="eutN"
                     /locus_tag="ACHOS5_004621"
     CDS             3891..4178
                     /gene="eutN"
                     /locus_tag="ACHOS5_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP9779324.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4190..5593
                     /locus_tag="ACHOS5_004622"
     CDS             4190..5593
                     /locus_tag="ACHOS5_004622"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP9779325.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPSETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5604..6440
                     /gene="eutJ"
                     /locus_tag="ACHOS5_004623"
     CDS             5604..6440
                     /gene="eutJ"
                     /locus_tag="ACHOS5_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP9779326.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            6430..7617
                     /gene="eutG"
                     /locus_tag="ACHOS5_004624"
     CDS             6430..7617
                     /gene="eutG"
                     /locus_tag="ACHOS5_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP9779327.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQVGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
BASE COUNT         1713 a         2014 c         2317 g         1609 t
ORIGIN      
        1 agtttatgca ccacatcaaa tatctctcca ttcttagtga tctacctcac cttttaaacg
       61 cgcttgccga attttgttat ttactctgac gaaaaattgt cacgatacac gaaagttttt
      121 cacaggcggc gactcatgga taaagaacgc atcattcagg aatttgtgcc cggtaaacag
      181 gtcacactgg cgcatctcat cgcgcatccc ggcgaggaac tggcgaagaa aatcggcgtt
      241 ccggatgcgg gcgcaatcgg cattatgacg ctaactcccg gagaaacggc gatgatcgcc
      301 ggtgatttag cgttgaaagc cgccgatgtg catatcggtt ttctcgaccg ttttagcggc
      361 gcgctggtga tttatggctc ggtgggcgca gtagaagaag cgttatcaca aaccgtcagt
      421 ggtttaggac gcttattaaa ttatacgctt tgtgaaatga ccaaaagtta atacgaggca
      481 ttcatgaaac gaattgcgtt tgtgggtacc gtcggggcag gaaaaacaac gttatttaat
      541 gcgttgcagg ggaattatac cctcgcaaga aaaacacagg ccgtggaatt taatgataat
      601 ggcgatatcg atacgccggg tgaatatttt agccatcccc gctggtatca cgccttaatt
      661 accacgctac aggatgtgga catgctgatc tatgtccacg gcgcgaatga cccggaaagt
      721 cggttgcctg ccgggttgct ggatattggc gtcagcaaac ggcaaatcgc cgtcatcagt
      781 aaaacggaca tgccagatgc tgatgtcgcc gcaacgcgaa agttattgct tgagacgggc
      841 ttcgaagagc cgattttcga gctaaacagc cacgacccgc aaagcgtgca gcaactggtg
      901 gattatctgg catctctcac caaacaggag gaagcaggtg aaaaaactca tcacagcgaa
      961 tgatattcgt gaagcacacg cgcgcggcga actggcaatg tccgtggttc tgcgcgccag
     1021 cattattacc ccggaagctc gtgaagtggc tgatctgttg ggttttacca tcaccgaatg
     1081 tgatgaatcg atcccggtaa cggcgtctgt tcccgccagc gcatcagccg ataaaaccga
     1141 aagccagcgc atccgtgaaa ccattatcgc ccaactgccg gaaggtcagt ttaccgaaag
     1201 cctggtcgcg cagctgatgg aaaaagtgat gaaggaaaaa cagtcgctgg aacagggtgc
     1261 actgcagccg agctttaaat cggtgaccgg caaaggcggc atcaaagtta ttgatggcag
     1321 cagcgtcaag tttggtcgtt tcgacggcgc acaaccgcac tgcgtaggct tgactgatct
     1381 cgtgaccgga gacgacggca gcagtatggc cgctggtttc atgcagtggg agaacgcctt
     1441 ctttccgtgg accctgaact acgacgaaat cgacatggtg ctggaagggg agctgcatgt
     1501 tcgccacgaa ggcgaaacta tgatcgccaa agcgggcgat gtgatgttta tcccgaaagg
     1561 ctccagcatc gaatttggta cgacatccag cgtgaaattc ctgtatgtcg cctggccggc
     1621 taactggcaa tccctatgaa agatttcatc accgaagcat ggctaagagc gaaccatacg
     1681 ctcagcgaag gcgcagagat ccatctccct gcggacagta gcctgacgcc ctctgcccgg
     1741 gagttactgg aaagccgcca tctgcgcatc aagtttattg acgagcaggg ccgcctgttt
     1801 gttgacgatg aacagcagca gccgcagccc gttcacgggc tgaccagtag cgatgaacat
     1861 ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa aaaagccgga tacgctgact
     1921 cacctgtcgg cggaaaaaat ggttgccaaa agcgatccgc gcctggcttt tcgcgctgtt
     1981 ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg aactggcgga accgtggcag
     2041 ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta tgcgtgccga tgcgctgggg
     2101 gaaccgctgg gcgatcaagc gattgtcggg ctttctgacg aagatctgca tcggctttct
     2161 caccagccgc tgcgttatct cgaccacgat catctggtgc cggaagccag ccacgggcgc
     2221 gatgcggcgt tgctgaatct gttacgcacc aaagtgcggg aaaccgaaac cgtcgcggcg
     2281 caggtcttta tcacccgcag ctttgaagta ttacgcccgg acattttgca ggcgctgaac
     2341 cgcctttcca gcacggtcta cgtgatgatg atcctgagcg taacgaagca gccgctaacg
     2401 gtgaagcaga tccaacaacg actgggggaa acgcaatgat tattgaacgt tgtcgtgaac
     2461 tggcgttgcg agcgcccgcc agagtagttt ttccggatgc gttagatcaa cgtgtgctga
     2521 aagctgcgca atatttacat caacaaggtc tggcaacgcc cattctggtc gccaatccgt
     2581 ttgaacttcg tcagtttgcg ctcagtcatg gcgtggcgat ggacgggcta caggtgattg
     2641 atccgcatgg caacctcgca atgcgggaag aatttgctca tcgctggctg gcccgcgcgg
     2701 gcgaaaaaac gccgccggat gcgctggaaa aacttaccga cccgctgatg ttcgccgccg
     2761 caatggtcag cgccggtaaa gcggatgtct gtatcgcggg caacctctct tccacggcga
     2821 atgtgctgcg tgccggatta cgcattatcg gcttgcagcc aggctgtaaa acgctctcat
     2881 ccattttcct gatgctgcca cagtacagcg gtccggcgtt gggctttgcc gattgcagcg
     2941 tggtgccaca gccgacggcg gcacaactgg cggatatcgc gcttgccagc gccgaaacct
     3001 ggcgcgccat caccggagaa gaaccgcgcg tggcgatgct gtcgttttcc agcaacggta
     3061 gtgcccgtca cccctgcgtt gccaacgtcc agcaggcgac agaaatcgtc cgtgagcgcg
     3121 caccaaagct ggttgtcgat ggcgagttgc agtttgacgc cgccttcgtg ccggaagtgg
     3181 cggcgcaaaa agcgcctgcc agcccgttac agggcaaagc caatgtgatg gtttttccgt
     3241 cgctggaggc aggaaatatt ggctacaaaa tcgcacaacg acttggcgga tatcgtgccg
     3301 tcgggccatt gatacaagga cttgccgcgc cgatgcacga tctctctcgt ggttgtagcg
     3361 tgcaggaaat tatcgaactg gcgctggtgg cagctgtgcc gcgtcagaca gaagtgaacc
     3421 gcgaaagcag tttacaaaca ctggttgaat gaacggtccc gttctggacc ctgataaaga
     3481 ggaaagcaca atggaagcat taggaatgat cgaaacccgg ggcctggttg cactgattga
     3541 ggcttctgac gcgatggtta aagctgcgcg cgtcaagctg gttggtgtta agcagattgg
     3601 cggcggcctg tgtactgcca tggttcgtgg tgatgttgca gcctgtaaag ccgcgaccga
     3661 tgccggtgcc gcagcggcac agcgtatcgg cgagctggta tctgtacacg ttatcccgcg
     3721 tccgcacggc gacctggaag aagtcttccc gatcggcctg aaaggcgata gcagcaacct
     3781 gtaatcacct tgtgtgacgt cgattcgggc gtcacgctct ctcctttttc attttacctt
     3841 ctgcggaggg tagggagagc ttttacctga aatacgccac ggaggcgggt atgaaactgg
     3901 cagtcgtcac tggacaaatt gtttgtaccg tacgccatca cggactggcg catgacaaat
     3961 tgctgatggt ggaaatgatt gatccacaag gtaatcctga cgggcaatgc gccgtcgcca
     4021 tcgacaatat tggcgcggga accggggagt gggtgttact ggtgagtggc agttccgccc
     4081 gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg cgtgattggc atcgtcgatg
     4141 aggtggtgtc tggcggtcag gtcattttcc acaaataagg cagaacatca tgaatcaaca
     4201 ggatattgaa caggtggtga aagcggtact gctgaaaatg caaagcagtg acacgccgcc
     4261 cgccgccgtt catgagatgg gcgttttcgc gtccctggat gacgccgttg cggcagccaa
     4321 agtcgcccag caagggttaa aaagcgtggc aatgcgccag ttagccattg ctgccattcg
     4381 tgaagcaggc gaaaaacacg ccagagattt agcggaactt gccgtcagtg aaaccggcat
     4441 ggggcgcgtt gaagataaat ttgccaaaaa cgtcgctcag gcgcgcggca caccaggcgt
     4501 tgagtgcctc tctccacagg tgctgactgg cgacaacggc ctgactttaa ttgaaaacgc
     4561 gccctggggc gtagtggctt cggtgacgcc ttccactaac ccggcggcaa ccgtcattaa
     4621 taacgccatc agcctgattg ccgcgggcaa cagcgtcatt tttgccccgc atccggcggc
     4681 gaaaaaagtc tcccagcggg cgatcacgct gctcaaccag gcgattgttg ccgcaggcgg
     4741 gccggaaaac ttactggtta ccgtagcaaa tccggatatc gaaaccgcgc aacgcttatt
     4801 caagtttccg ggtatcggcc tgctggtggt gaccggcggc gaagcggtag tggaagcggc
     4861 gcgcaaacac accaataaac gcctgattgc ggcaggtgcc ggtaacccgc cggtagtggt
     4921 ggatgaaacc gccgacctcg cccgtgctgc tcagtccatc gtcaaaggcg cttctttcga
     4981 taacaacatc atttgtgccg acgaaaaggt actgattgtt gttgatagcg tagccgatga
     5041 actgatgcgt ctgatggaag gccagcatgc ggtgaaactg accgcagagc aggcgcagca
     5101 actgcaatcg gtgttgctga aaaatatcga cgagcgcgga aaaggcaccg tcagccgtga
     5161 ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc ggcctgaacg tcccgtcaga
     5221 aacgcgactg ctgtttgtag aaaccaccgc agaacatccg tttgccgtga ccgaactgat
     5281 gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg gatgccattg cgctggcggt
     5341 gaaactggaa ggcggttgcc accacacggc ggcaatgcac tcgcgcaata tcgaaaacat
     5401 gaaccagatg gcgaacgcca ttgataccag cattttcgtt aagaacggac cgtgtattgc
     5461 cgggctgggg ctgggcgggg aaggctggac cacgatgacc atcaccacgc caaccggtga
     5521 aggggtaacc agcgcgcgga cgtttgtccg tctgcgtcgc tgtgtattag tcgatgcgtt
     5581 tcgcattgtt taaggagtaa ccgatggcgc acgatgaaca atggctcacc ccacgtctgc
     5641 aaacggcggc aacgctgtgt aaccagacgc ctgccgcgac ggaatctccg ctgtggctgg
     5701 gggtggatct gggcacttgc gatgtggtgt cgatggttgt cgaccgcgac ggtcagccgg
     5761 tggcggtatg cctcgactgg gctgatgtgg tccgcgacgg catcgtctgg gatttcttcg
     5821 gtgcggtcac cattgttcgt cgccatctcg acaccctcga gcagcaattc ggtcgccgtt
     5881 ttagccatgc ggcgacctca tttcctcctg gtacggaccc gcgtatttcc atcaacgtgc
     5941 tggagtctgc cggactggaa gtgagccatg tgctggatga gccaaccgca gttgcggatc
     6001 tgctgcaact ggacaacgcc ggtgtagtgg atatcggcgg cggcactacc ggcattgcca
     6061 tcgtgaaaaa gggcaaagtg acgtactcgg cggatgaagc caccggcgga catcacatct
     6121 ctctgactct cgccggaaat cgccgtattt cgctggaaga ggcagagcag tacaaacgcg
     6181 gtcacggtga cgagatttgg cccgcggtga aaccggtgta cgaaaaaatg gcggacattg
     6241 tggcccggca tattgaaggg cagggcatta cggatttatg gctggcgggc ggttcctgta
     6301 tgcaaccggg cgtagcggcg ctgttttgca aacaatttcc ggcgttacag gtgcatttac
     6361 cgcagcacag tttgtttatg accccgctgg caatcgccag tagtgggcga gagaaagcgg
     6421 aggggatcta tgcaaagtga attgcaaacg gcgctttttc aggcgttcga taccctgaat
     6481 ctgcaacggg taaaaacatt tagcgttcca ccggtgacgc tttgcggtcc gggcgcggtg
     6541 agcagttgcg ggcagcaagc gcaaacgcgt gggttgaaac atctgttcgt gatggcagac
     6601 agctttttgc atcaggcagg gatgaccgcc gggctgacac gtagcctggc cgttaaaggt
     6661 atcgccatga cgctctggcc atgtccggtg ggcgaaccgt gcatcaccga cgtgtgtgca
     6721 gccgtggcgc agttgcgtga atcaggttgt gacggggtga tcgcgtttgg cggcggctcg
     6781 gtgctggatg cggcgaaagc cgtggcgttg ctggtgacga acccggatag cacgctggca
     6841 gaaatgtcag aaaccagcgt tctgcaaccg cgcctgccgt tgattgccat tccaacgacc
     6901 gccggaaccg gctctgaaac gactaacgtg acggtgatta tcgatgcggt aagcgggcgc
     6961 aaacaggtgt tagcccatgc ctcgctgatg ccggatgtgg cgattctcga cgccgcgttg
     7021 accgaaggcg tgccgtcgca tgtcacggcg atgactggca ttgatgcgtt aacccatgcc
     7081 attgaagcgt acagcgcgct gaacgctaca ccgtttaccg acagcctggc gattggtgcc
     7141 attgcgatga ttggcaaatc actgccgaaa gcggtgggct acggccatga ccttgccgcc
     7201 cgcgagagca tgttgctggc ttcatgtatg gcgggaatgg cgttttccag cgcggggctt
     7261 gggttatgcc acgcgatggc gcatcagccg ggggcggcgc tgcatattcc gcacggtctg
     7321 gcgaacgcca tgttgctgcc aacggtgatg gagttcaacc ggatggtctg tcgtgaacgc
     7381 tttagtcagg ttggtcgggc gctgcgaact aaaaaatccg acgatcgtga cgctattaat
     7441 gcggtaagtg agctgatagc ggaagttggg attggtaaac gactgggcga tgttggtgcg
     7501 acatctgcgc attacggcgc atgggcgcag gccgcgctgg aagatatttg tctgcgcagt
     7561 aacccgcgta ccgccagcct ggagcagatt gtcggcctgt acgcagcggc gcaataaatg
     7621 ccggatgcga cgcttgccgc gtcttatccg gcc
//
LOCUS       DAZKHK010000185         7393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKHK010000185 DAZKHK010000000
VERSION     DAZKHK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.186"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1257
                     /gene="yqcE"
                     /locus_tag="ACHOS5_004625"
     CDS             <1..1257
                     /gene="yqcE"
                     /locus_tag="ACHOS5_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP9779328.1"
                     /translation="RWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSNTEIGLIM
                     STFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVMLCIQVAFA
                     ITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFSRFAPDDS
                     ASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVLRISTTWY
                     CSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPLGGIITTY
                     SKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYASRGLYWAC
                     PGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWLMGMAALG
                     MVIIFTFLLFQKIRTADSAPAMASSK"
     gene            1284..2762
                     /gene="ygcE"
                     /locus_tag="ACHOS5_004626"
     CDS             1284..2762
                     /gene="ygcE"
                     /locus_tag="ACHOS5_004626"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9779329.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRQMLFDVQMPGTILGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKNVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(3297..3968)
                     /gene="queE"
                     /locus_tag="ACHOS5_004627"
     CDS             complement(3297..3968)
                     /gene="queE"
                     /locus_tag="ACHOS5_004627"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP9779330.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            4141..4743
                     /locus_tag="ACHOS5_004628"
     CDS             4141..4743
                     /locus_tag="ACHOS5_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFP9779331.1"
                     /translation="MFRIFIAFIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRT
                     DLIPNLVASIKGYSSHEQEVLEAVTLARSQANRASSDLQKTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPQLKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVTAVSKAPTIDFSQNANAH"
     gene            4757..5428
                     /locus_tag="ACHOS5_004629"
                     /pseudo
     CDS             4757..5428
                     /locus_tag="ACHOS5_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            5665..6813
                     /locus_tag="ACHOS5_004630"
     CDS             5665..6813
                     /locus_tag="ACHOS5_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9779332.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQSALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQLSLPCLAWTVLMIVAIIVLWRFTSRPGPGIWMISMITPMVWFFFFQ
                     DDVIIRRISLVCFSLFFAATCWQRLAVIFHMYRVFPMMLAPKNKNAKVKKQKTTQQVS
                     QSERNSLFVVLLIIVVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGHKSNRKTGNRNAHSRSSSKNSFRGGGGSSGGGGSSDRW"
     gene            6819..>7393
                     /locus_tag="ACHOS5_004631"
     CDS             6819..>7393
                     /locus_tag="ACHOS5_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9779333.1"
                     /translation="MRIILFLLTLWCLPVLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITQKTRAQVAVLIVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPF"
BASE COUNT         1963 a         1680 c         1754 g         1996 t
ORIGIN      
        1 cgttggataa ccctcgcgat aattagtttt agcggcggcg ttagtttcga cctggcttat
       61 ttacgttata tttatcaaat tcccatggca aaatttatgg gattcagcaa taccgagata
      121 ggtttaataa tgagtacctt tggtattgcg gctattattc tttatgcccc cagcggcgtt
      181 attgccgata aattttcaca tcgcaaaatg attacttccg cgatgatcat taccggatta
      241 ctgggtctgt taatggcaac atatccaccg ctgtgggtaa tgctctgtat tcaggtcgcc
      301 tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc atcgttgctg
      361 ggcgatcata gcgagcaagg gaaaattatg ggctggatgg aagggctgcg cggcgtcggt
      421 gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc gctttgcacc ggatgacagc
      481 gccagcctga aaacggtcat tattatctat agtgtggttt acattctgct gggcattttg
      541 tgttggtttt ttgttagcga taacaacaat ctgcgcagtg ccaataacga agaaaaacag
      601 tcattccagc ttagcgacat cctggccgtt ttgcgcatca gcaccacctg gtattgcagc
      661 atggtgattt ttggtgtctt caccatctac gccattctga gttactccac caactatctg
      721 accgaaatgt acggcatgtc actggtggcg gcgagctaca tggggattgt gatcaacaaa
      781 atcttccgcg cgttatgcgg cccacttggc ggcattatca ccacctacag caaagtgaaa
      841 tcccctaccc gcgtggtcca aatcctttcc atcatcggtt tgctggcatt gacagccctg
      901 ctcgtcacga actctaaccc gcaatcggtc gcgatgggga ttggcttgat tttattactg
      961 ggattcacct gttacgcctc acgcgggctg tattgggcct gccctggcga agcgagaaca
     1021 ccgtcttaca ttatgggcac cacggtaggt atttgttcgg tgattggatt tctgccggat
     1081 gtcttcgttt acccgattat cggctactgg caagacaccc tgcccgctgc agaagcctac
     1141 cgcaacatgt ggctaatggg catggcggcg cttggcatgg tgatcatctt tacctttttg
     1201 ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa tggctagcag caagtaagcc
     1261 aggaaaatta caggagaaaa atcatgtcga aaaaatacat catagggatt gatggcggaa
     1321 gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa tgtggtttgc gaaggcaaag
     1381 gcttattgca gccgatgcac acgccagatg ccgataccgc agaacatcct gacgatgatt
     1441 tatgggcatc actatgtttt gccagtcacg atttgatgag ccagtttgcc ggaaataaag
     1501 aagatattgt cggcattggc ctgggctcaa tccgttgctg ccgtgcgtta ttgaaagccg
     1561 atggtacgcc agccgcgccg ttgattagct ggcaggatgc acgcgttaca cgcccttacg
     1621 aacacactaa tcctgacgtg gcctatgtca cctctttttc gggttatctg acacatcgct
     1681 taaccggcga gtttaaagac aatatcgcca actattttgg tcagtggccg gtggattata
     1741 agacctgggc atggagcgaa gatgccgcgg taatggaaaa gtttaatatc ccccgccaga
     1801 tgctttttga cgtgcaaatg ccgggcacca ttcttgggca tatcacacca caagctgcgc
     1861 tggcgacaca tttcccggct gggctgccgg ttgtttgtac caccagtgat aaaccggtag
     1921 aagctctggg agccgggtta ctggatgatg aaacggcggt aatttctctt ggcacctata
     1981 tcgcgttgat gatgaacggc aaagcactgc cgaaagatcc ggtcgcgtac tggccgatta
     2041 tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg tattcgcaaa ggcatgtgga
     2101 cggtgagctg gctgcgcgac atgttaggcg agtcgttaat tcaggatgcc aaagcacagg
     2161 atctttcacc ggaagatttg ctcaacaaaa aagcgtcttg cgtgccacct ggttgtaatg
     2221 ggctgatgac ggtgctggac tggctgacca atccgtggga accgtacaaa cgcggaatta
     2281 tgatcggctt tgattccagt atggattacg catggatata tcgttcgata ctggaaagcg
     2341 tggcgctgac gctgaagaat aattacgaca atatgtgtca tgaaatgaat cactttgcaa
     2401 agaatgtgat cattactggc ggcggttcga acagcgatct gtttatgcag atttttgccg
     2461 acgtgttcaa ccttccggca cgccgaaacg ccattaacgg ttgtgcaagt ctgggggctg
     2521 cgatcaatac ggcggtaggt ctggggctat acccggatta cacaacggct gtcgataaaa
     2581 tggttcgcgt gaaagatatc ttcataccga ttgagagcaa tgccaaacgc tacgacgcga
     2641 tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg aaaaaatcgt
     2701 atgaagtgat gcatggggaa ttggggaatg cggattcgat ccagagctgg tcgaatgcgt
     2761 aagtgaggaa ggccgggcgg gaaactgtcc ggcctgaaca tacctgaatg gttatccccg
     2821 ctgacgcggg gaactcatat ccggtttttg ctggtgggcc gcgccgttcg gtttatcccc
     2881 gctggtgcgg ggaactcgtt caaatgcgcc catgtggttc gtgggcgacc ggtttatccc
     2941 cgctggtgcg gggaactctc taaacataac ctattattaa ttaattattt tttaagccag
     3001 tcacaatcta ccaactttac agtatcacac aaacaacaca tccattatgt taaagagcac
     3061 ttaatccatt gattaaaaag ataaatattt aaaataactc tattcaaact aaaatctacc
     3121 aaactttacc gcaataattt tcactccagc gaaaaattaa cgccacagaa tttgtagaga
     3181 atgataatga ttagtccaga atgggcaaaa tggaggtttt tcagagacga caaagaatat
     3241 aaatgagtag aagtctttac agattccacc catttataac gcttataaat gtttaatcag
     3301 gcaatattca aatatttatg tgtctgcatc gacaaacgcc agttgcgcgc aatgcaggta
     3361 tcgatacata aacgcgtagc atcttccttc tggctaattg gctgaagtgc aatgactcgc
     3421 ggtttatcat cggtcagcgt cgccagcaat tcatccagtg cttcaatatc acgtacgcgt
     3481 cctactgggt gtttgatttc gttggctcgc tccagggctt gtgacaacac ttcatagcca
     3541 ccgcgcatat ttagctttgg tgatacggta acccaggtat tcggtgtgca gcgtacttca
     3601 tgagtaccac tggtttcgat ctggcagcta aaaccgttct tttcgagcag gtcagtcagt
     3661 ggcagcaaat catgaatgca aggctcacca cccgtaatca ccacatgacg cgcggtgtat
     3721 ccctgacggc caatgacagc cagcaaatct tcactgctcg ccgcccccca cttatcactc
     3781 tctttggtct tcgccagaat gctgaaaagg gagacttccc gatcctcaag cttttcccag
     3841 gtgtgtttgg tgtcgcacca ggcacagcca accggacatc cctgtaaacg aataaaaatg
     3901 gcggggacgc cggtaaagta accctcacct tgcagggtct ggaacatctc gttaatcggg
     3961 tactgcatag cattctctgt gaagtggata attgttaatt attgcagatc ctgccacaac
     4021 aatcatgtct tattaacatt ctgttacagg caggtttgat gcaaaaattg gctcagatca
     4081 ttaagaataa aatgaagcgg cgtattatgt tttcaaggat taaatgaaag ggatttaaac
     4141 atgtttagaa tattcatcgc gtttatattt attttcaatt tatcaggttg cggttataac
     4201 gatattcaaa cctatgatga acaggttaat gcctcctggt cagaagtatt aaatcaatat
     4261 caacgtcgca ccgatcttat tcctaatctt gtcgctagta ttaaaggtta ctccagccat
     4321 gaacaagagg tactagaagc cgtaacattg gcacgtagtc aggctaatcg cgccagtagc
     4381 gatcttcaga aaactccggg agatgaacaa aaattacaag cctggcaaca ggcgcaggcg
     4441 caggtgactc gcactctcgg gcagttaacg attattagtg agcgttaccc tcagttaaag
     4501 gctcaagagc tctatcaaaa cttaatggta cagctcgaag gaagtgaaaa tagaattgcc
     4561 gttgcgcgag gaagatatat taaagccatt gaacaatata atgtcactat ccgcaaattc
     4621 cccgctgtat taaccgcaaa ggtcatggat tacacaccaa agaaaaatta tttgccggat
     4681 gatgtcaccg cagtaagtaa agccccaaca atagatttta gccagaatgc taatgctcat
     4741 taagcgggga cgttctatgc gaaattttat cttccttatg gctttcttct gttcatctgt
     4801 gtttgctaca caaattccag tacctgaatc acccaggtat gtgaatgact taaccggcac
     4861 attaacaaac agcgaagtaa acaccctgac caaccagatt aaagccctga cgcaaaaaaa
     4921 ccacgcacag ttggtcgtgt tagtggttga tacaacaggc gatgaaacca ttgagcagta
     4981 cgcaacgcgg gtattcgata gctggaaacc gggtgataaa gaccgtgatg atggcgttct
     5041 actgttagtc gcctggcagg atcataccgt gcgtattgaa atcggttacg ggctggaagg
     5101 tattattacg gatgcacagt ccgggaaaat tattcgcaac agtattattc cggcatttaa
     5161 aaaaggcgac ctggctggtg gtttacaaaa ggggatcaac gatattgaaa gtcgcctgac
     5221 ggggaataat tcagcaacga taacaccgac cgatcaccct ttgcccttta gcggttggtg
     5281 ggctttatta gtctgggcga taatactgac gtttatttct gccagaggat atatcaaaac
     5341 gttgggagta atttgcttcg cagccattgt actggccttt gttttaccta tagcaggttt
     5401 cagcggaagc tggggagtac tcacaacatt actctgcttc gccacaccgt ttctggcagt
     5461 tgccatcgct tttaccccct tcggcaaaaa ggttcgggat tcgatgcgtg atgccaacca
     5521 gccatcacgc catacaagaa gcaattcttc ctcccactat tcatcatcgt catcctcttc
     5581 attcgacaat gacaatttta gcggtggtgg cggttcatct ggtggcggtg gcgcttccgg
     5641 gcgctggtaa caaaaggaat tgatatgcgt atttttctct tacttctgat gctatgcggt
     5701 tttcaggtat tcgctgaacg attgccttca gtgactgact taaccgggac attaacgaca
     5761 gaagaacaaa gtgctttaaa tcaacaatta cagactctcg agcagcaaaa ccacttccag
     5821 gtggccgtgt tggttacgcc gacaacaggg ggcaaaaaca ttaaacttgc agcctcgcag
     5881 agatacgggg tatatcactg gaaacccgtt ggagagaagc gatatggcga aggtatctta
     5941 attctcgtcg tttggccgga agggctggcg tcaatgaaga ttggacatgg actggaacag
     6001 atgttgccgc cggaacaagc tgcgcaaatt gttcgttacc atatgcagcc agaattcgaa
     6061 aaaaacaatc tctttgctgg attaacgggc ggaattgaaa gcatcgcaca gtttacccat
     6121 atcaaagcga accttagccc tttggatgct ttagccaatc atctgtttgc taatccgcaa
     6181 ctttcgctgc catgccttgc ctggacagtg ctgatgatcg ttgccatcat tgtcctctgg
     6241 cgatttacca gccgtccagg acctggcata tggatgatat cgatgattac tcctatggtc
     6301 tggtttttct tctttcagga tgatgtcatc attcggcgga tatcgctcgt ttgttttagt
     6361 ttatttttcg cagcaacctg ctggcagcgt ctcgcggtaa tttttcatat gtatcgcgta
     6421 tttcctatga tgctggcccc aaaaaataaa aacgctaaag tgaaaaaaca aaagacgact
     6481 cagcaggttt ctcaatccga acgaaactca ttgtttgtcg tgctgctgat aattgttgtg
     6541 ggatggtgcc tggtattcgc cagtaataaa gatatagcat tcatctcaac aattattggg
     6601 ctaattgatc attttatcgg cccaataacc attgctggca tcgttctgct gatcattgtg
     6661 gccaaattta ctggcaatct taaactggga tcaggacata aaagcaacag gaaaacgggg
     6721 aatcgtaatg ctcattctcg ctcttcgtcg aaaaattctt tccgcggtgg cggcggttct
     6781 tcaggaggcg gcggttcatc cgaccgttgg tgatcgttat gcgtataatc ctttttctac
     6841 tcactttatg gtgccttcct gtgcttgccc agcaaattgc tgtgccggag ctgcgccaac
     6901 aggtgaccga tattactggt accttaagca cgtctgaaca acaatcacta acacaacagc
     6961 tgcaggatat tacacaaaaa acacgggcac aggtcgctgt attaatagtg ccatccaccg
     7021 gagacgattc tattgaacaa tatgctacaa gagtttttga caattggcgt ttaggagatg
     7081 ccaaacgcaa tgatgggata ctgatcattg ttgcctggtc ggatcgcact gtccgcatcc
     7141 aggtaggtta tgggctggaa gaaaaggtaa ccgacgctct ggctggagat atcatccgta
     7201 gcaacatgat acccgcattt aaacaacaaa aattagctaa gggattagag ttagctataa
     7261 tcgctttgaa taaccaactc acttcacaac accaatattc ggttaatcct tcagaaagtg
     7321 aatcagcatc ttccagcgat cattactact ttgctatttt ttgggtattt gcagtgatgt
     7381 tcttcccttt ctg
//
LOCUS       DAZKHK010000186         7326 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKHK010000186 DAZKHK010000000
VERSION     DAZKHK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.187"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..578)
                     /locus_tag="ACHOS5_004632"
     CDS             complement(<1..578)
                     /locus_tag="ACHOS5_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779334.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLAEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEGE
                     QKPVQPEGGQEAGKTEVSSTASTEAGSGSSSD"
     gene            complement(863..2110)
                     /locus_tag="ACHOS5_004633"
     CDS             complement(863..2110)
                     /locus_tag="ACHOS5_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP9779335.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            complement(2170..4317)
                     /locus_tag="ACHOS5_004634"
     CDS             complement(2170..4317)
                     /locus_tag="ACHOS5_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP9779336.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAVLFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKRLSGKQQSEESRPAAS"
     gene            complement(4322..5485)
                     /locus_tag="ACHOS5_004635"
     CDS             complement(4322..5485)
                     /locus_tag="ACHOS5_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP9779337.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNIFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ENELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            5868..6068
                     /locus_tag="ACHOS5_004636"
     CDS             5868..6068
                     /locus_tag="ACHOS5_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779338.1"
                     /translation="MIAGTKNNINQQDRKSSQQRERSVDKEWDGLLTSYHIIDIFYPA
                     NSNGIMYDLASGNYHRQKNNIL"
     gene            complement(6272..7097)
                     /locus_tag="ACHOS5_004637"
                     /pseudo
     CDS             complement(6272..7097)
                     /locus_tag="ACHOS5_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1865 a         2021 c         1557 g         1883 t
ORIGIN      
        1 tggtcggaag agcttccact tcccgcctca gttgaggctg tggatgatac ttctgttttt
       61 ccagcctcct gaccgccttc aggctgaacc ggcttctgct caccttcagt tgcacgctta
      121 tgacgactgc ttccaccacc gcctccggca gccagtgcaa tagccgccag ccctgcacca
      181 aaagcaatcc ccccccagat aatcccgtta ttgatacctt cagccagtgt gccactttgc
      241 cctgcagtaa aattctccag tacaggacca tcctgggtca ggacagcttc agttacctga
      301 ccattttcac ccacaacatt aaacacggct gtacactgtg tttcacccgg ctggcattct
      361 tcccagaatc cctcaatccg gatttctgta tcatcttcct ttttacgcag aattaaatca
      421 tcaccagaac ggtccgcttc cacaccatcc gggaccagac ctgtcacctc atcaaccagt
      481 ccatactgtt ccccggcctg tgcctttaca gtatgacggg ctccggctgt cagtactgtt
      541 ttttctccac tgaccttgcc ggtgaaaatc attttcatat aagtatcctc aggttaataa
      601 aaccagtatg ctcttatgtt ttatccgcta aagggagtca ttaaataaaa acgaatcaga
      661 ttacagataa tttatttctc tggccggacc tccttaatca ttccgctttt tcagatttat
      721 tttcccctga cagatataac atttatatta tcttcagccc caaatcacat acaggcatgc
      781 gcaatgaccc gggcagaata aagtgaaatc attctgcccg tttaaaattg atttaaaata
      841 ctttccccgg aagacctttt tattatcttt cctgaagtgc ctgcttcatt ctggttatcg
      901 gtttaatcag atactgaaat accgttttac gcccggtttt tatatccacg ctggctatca
      961 tgccaggaat aactggcaat ctttttccgt tcctgtctgt cagataatta ttactggtgg
     1021 taaccagtac ccggtaataa gcttcattcg ggtccagttt taaatctccc ggccgtttct
     1081 ggtcacgtaa agtatccgga cttaccagtg tcaccacgcc gtcaagacca ccataaatgg
     1141 catagtcata agccgtcagt tttacaagtg ccggcatccc tgttctgaca taggcaacat
     1201 cccgtggatt tatataagct tcaatcagca actggtcttc cagcggaata atttccatta
     1261 tgtcctgtcc tgcagaaacc acccctccca gtgtgtttac ccgtatattt tttacgatac
     1321 ctctcagggg ggccctgaga cgtgaacgct caaccgggtc ggctctgcca gccatattct
     1381 cttttgcctg agcaagctct gcttctgtct tcaccagttc agcaccggct tccgtcaggt
     1441 atttattctg tttctcatcc atctgtaact gcagctccgc tgactgacgc tgcatacgta
     1501 acagttcgac ctccgacatt gccccttcac gcacaattgg ccgcgtcatt gctatttccc
     1561 tgtccagcag cgccttactc tgcttcagac cggcaatgct ctgcgccagc tccgtcttcc
     1621 ggagaaaata gaccgtactt tcccgctccc gcaaatcctc aggaacgtta tccggaaatg
     1681 tcagtgattc ggaataagct tctgcacgga gtcttgccct gacggcctcc aggttattca
     1741 cccgggcttc actttccctc agcatggcag agctccgggt atcatccagt gtcagcagta
     1801 cctgaccttt ctcaacaata tcgccttccc tgacctcaag tgatttcagt atacccgggt
     1861 caagggtctg tataacctgc tccctgctgc cgggaataat actcccctga cctcgggtca
     1921 cttcatccag aggactgttc catgcccaga taacaaacgt cactaccagt accagcagaa
     1981 aaatcaccat caggaaaagg gcatgatgct tttcctgctg aagagctgcc tgtacatctg
     2041 tcaacagcgc caagtctgac tgcctgacca cctctgacga cgctttattc cgttccgttt
     2101 tttctgtcat atccctgcgg tccacaaact gttaaacata tctgctcttt tttcttatcc
     2161 ggttgacgtt caggaagctg ccggtctgct ctcttcactc tgctgtttcc ctgacagtct
     2221 tttcagtatt tcatcacggg gaccgtccat cattacccgc ccttcattca tcacgacaat
     2281 acggtcaaca atattcagaa cctgcagacg atgtgtcacc accaccattg tgcgtccccg
     2341 gctccagtgg ctcagacctt tcagaacaac agactcagtc tgcaggtcca gacctgtcgt
     2401 gggctcatca agcagtacca cccggggaga acccagtacc attctcgtca gggcaaccat
     2461 ctgcctctgt cctcctgaca gcccctgacc gccttcccct gtctgcatat caagtccgcg
     2521 gggatgatgc cggataaaac ggtcaaaccc gaaacgtccg gctgcattaa tcatatccac
     2581 atccgacagg cttcccggca gacgcgaaag ctgcagattt tcacgcagag ttcccagaaa
     2641 aagccgggga ttctgttcca gaagaaacac tttatcccgg acaaaacagg ggtccagttg
     2701 ctgcatcgca accccgtcaa gagtaatttg tcccctgttc tgacggtaca gccccaccat
     2761 cattttcagc agcgtcgatt taccacaacc tattgagcca ataataccca cccgctctcc
     2821 gggccggata cagagcgaca aaccgcacaa tgcgtcgggt aatccgggct tataagaaaa
     2881 ggagacatca ctgaaacgaa actctccctg gatatccttc actgtcagat aatgtgtctg
     2941 actttcccgc tccaccggac ggcggacaat gtcattcacc ccctgcatgg ccaggcgggc
     3001 ctgctgaaaa cgggtggcca gtgatgctat ctgaccaagc ggtgccagcg cacgaccgga
     3061 aagaataact gcggcaatca gtgcccccat ggtgattcgg ttatgtgggt catcactgtg
     3121 aataaggtac gtccccagaa tgaccagcag aacagtattc agttgctgaa ctgctgtgga
     3181 aaagcagatt atgacattac tggtattttt tacccgtatg gacgagcggg cgacactggc
     3241 tgtatatcca tcccagcggc gctggaccca gttaactgca ttattcagct tcagggtttc
     3301 tattccgtcc agtgcctcca caagtaatcc ctggcgctgc gatgattccc gcatggaggc
     3361 attaatatga cgcgaaagcg gtcgctgcag taatattcct gccagaatca ccagcggaac
     3421 cagtgtaaga ggaacaacag caagccagcc ccccacaagc ccgatgacac caatgaaaag
     3481 caggataaac ggcaaatcca caaaagccag cagcgtggcg ctggtcataa actccctgac
     3541 agactcatat tcccgcaaat tgctggcgta tgaaccggaa gacgccggcc tgtccgacat
     3601 tttcactgcc atcacccgcc ttaacagtgc tgagctgata atcaggtctg ccttcttacc
     3661 cgcaatatcc gtcagatagc tccggattaa ctttgcagca cactcaaaca gaacggctgc
     3721 aatcaccccg atactcagca tccataacgt ttcccaggag cgattgggta tgacccggtc
     3781 ataaacgttc atgacataca gggaactgac cagcgcaaga aagttaatga taaaactcgc
     3841 aagaatgacc tgtccgtaat atggcctgaa ccgccacagt acccgccaga accatgcccc
     3901 ggtcatgtca tactccggca gttccgaacg gcgatcctgt gttgcctgtg gctgaacaaa
     3961 ccagcagtaa cctgaataaa cagccattaa ttcatcgtga gaaagtgtct tacggagtcc
     4021 cccgggctgg agtacatgat actgccgggc caggccggca ccctcaatac gcaccaccac
     4081 aatggcttcc tgtgcattca gtaaaagaag cacaggaacc gcaagtgaag gaatatcttc
     4141 cggagcccgc cgggaaagat gattatcgaa cccttcactg cgaagaaact cgccaacaga
     4201 acgaaaatca atcccggaga catctgtccg gactgtaccg gcagccagcg tctcagcaga
     4261 tacaggtgta cccagtaatg ccgttacctg tgataatcct tcaaaaatac tcttcactta
     4321 tttatctgtc cttctgccct gttacttttt cttctgcacc agaaagattc actccgctcc
     4381 atttcaggag cattccctgg gaatacagat aatccagcat tccgtggagc atctgatcct
     4441 ctgtctgaac ccgggaaagc ccgacgctgt acagttcgtt ttctgcattc agtaattcaa
     4501 tcagtgtttt tcttgccacc cggaactgaa gacgataaaa gtccacaact ctggcagagg
     4561 cctgctcctg acgtctgagc gtttccattt ccatccgact ttgtctgacc gttatcagcg
     4621 ataatcgtcc ggtttcatca atcattcggg caacagagtc cagtcgtccg gtagctgcag
     4681 ctatctgcgc ggccttttca ctgacaccat aactggcatt acggttgaac aggtcccagg
     4741 acagactgac acctatctgc ctgtcatccc gggtcactga tcccgttaaa tccacccggg
     4801 gataccgggc aagctcctgc gctttcagcg ccgctttttt actctctaca tcagcctgac
     4861 tggcaagcca cgacggattc cctttctgag gcgtctgcgt aaaatcagtt aacaatctgg
     4921 actctgtcat ccgggaaaaa ggattttcaa gatcggcttc ggttaccgga tttgtggtcc
     4981 aggtcgaaag ttttcccagc gtggttttga gcactcgggt gcgggtattt atctgctgcc
     5041 ggaccatcag cctgcgggct tcggcctgaa caaattcaga ttcccgcccg gtatcgtgaa
     5101 tactgatggt gttaagatca ttaagaattg cgttatgccg ggagagactc tgttcaagta
     5161 ccgcaatgga ttgcttttcc cttagcgcat caagatataa tgaaacaaag gagcgaattg
     5221 tttcctcgcc agcctcatca actttataac ggtaatactc ggcttccgac tcactccggc
     5281 tgacatccgc ctcaatagct cccgaagcga atatattcat ttcaccgcgg ataccgggca
     5341 aaatatcttc agagtcgtag tcatagtcgt agcggtgact ctgggaaagg agtttactgc
     5401 ctgttgctgt gacgaccggc cagtgtgctg aacgagcctg ctccacacgg gaacgggcag
     5461 aggtggcttc tgcccttgcc tccatcactg atggatcatg aatgagagca tccctcagga
     5521 gggccggcag tggagaagcg aacacgttgt ggacagagaa aagaaaacag aaagataata
     5581 ttctcttcac ccgaacctcc gggacacgcg ggaaatataa tcaggcatca tgatgcaacc
     5641 gggtaaaata ttgagtaaaa ttgaaatgca gagacaccgg aattgatcca agctaccgcc
     5701 attatcttcc gcgctgacag aagataataa aaatgtgaga tacttcaatt ttattgaaaa
     5761 cctgaaaaac ccttacaatc cttgcagttt atgaagaaaa ttttcacaaa gtaaatttaa
     5821 aaataaacaa attaaatgta atataatcgg gggaggatta accatcgatg attgccggaa
     5881 caaaaaacaa tataaaccaa caagatagaa aatcatcaca acaaagggaa aggtctgtag
     5941 ataaagagtg ggatggactt cttacttcat atcacataat tgatattttt tatcctgcaa
     6001 actccaacgg cattatgtat gacttagcct caggcaatta tcacaggcaa aaaaacaata
     6061 tattgtaagc ttcatagaat ttggaacttt atataataaa taattctgag ttttttatca
     6121 ccattatatt aacaaaatta atgtattcca gagatttcac attagtctcc aatttttcag
     6181 aagaatctat ttacctggca ggaaaagtca tctggtgttt cgccggagtg aggggtacag
     6241 gcaatagcgg acataaccat ttgttctttt tctaagcagc catctgataa tattttatcc
     6301 tgaaggctgt cggggagata ttccccagac gagagtgacg acgctgacga ttgtagaaaa
     6361 tctcaatgta ttcccgtatt actgagatgg cctcatcccg gcttttaaaa cgatagtggc
     6421 tcagactttc atttttcagc gttccccaga agctttccat cggagcattg tcgtaacagt
     6481 taactttacg cgacattgat attttcagac caaactgctc ctgtatggcc ctgtaatcgt
     6541 atgcgcagta ctgtgaacca cgatcggagt ggtggattag cccggtaggt gagcgctggt
     6601 tcctgagcgc cataaacagg gctttacctg tcagctcttt tgtcatgcgc tctcccatgg
     6661 cgtagccgac aatttcgcac gtataaacat ctttgacgcc agcgatatac agccatcctt
     6721 ccggggggca acatacgtta tatctgcaac caagacctga ttaggtgctg taggcgtaaa
     6781 gttctgattc aggagattcg gcgcaaccgg cagattgtga ccagagttcg tgttcgccct
     6841 gaacttgcgt ttcagcttac agtgcagcct cagctcctta cgaagacgtg ccagtctgtc
     6901 acgaccgacg ataattccgt tatctgccag ctccgtctgg agcagctagg ttccatatgt
     6961 ttcgcgtgtg cggatatgtg ccaccttaat ctccagtttt agccgctcat cactttgttt
     7021 tctgtctgag ggtcatgctg tactcagtcg taataaccga tcctgtatac accaagtacc
     7081 cgacacatcg cttcaataca taatcgcatc tgaatcgttc accgattcgc ggcatttttc
     7141 aaggtagttg tcgccattgt tcgactaact gatcgacatt acagcctaga gcctagctgg
     7201 gggctgtgca caaaaaagcc ccggacattc cggggctaca ctgcatatta acgaacgctc
     7261 tgtccggtta tttcaaccct gcggttgggg cgcagacatt ccttcagtgc ttcgccctcc
     7321 acatcc
//
LOCUS       DAZKHK010000187         7310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKHK010000187 DAZKHK010000000
VERSION     DAZKHK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.188"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            75..206
                     /locus_tag="ACHOS5_004638"
     ncRNA           75..206
                     /ncRNA_class="other"
                     /locus_tag="ACHOS5_004638"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(391..1068)
                     /gene="narI"
                     /locus_tag="ACHOS5_004639"
     CDS             complement(391..1068)
                     /gene="narI"
                     /locus_tag="ACHOS5_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9779339.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIDVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1068..1778)
                     /gene="narJ"
                     /locus_tag="ACHOS5_004640"
     CDS             complement(1068..1778)
                     /gene="narJ"
                     /locus_tag="ACHOS5_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9779340.1"
                     /translation="MIELVIVSRLLEYPDAASWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1775..3313)
                     /gene="narH"
                     /locus_tag="ACHOS5_004641"
     CDS             complement(1775..3313)
                     /gene="narH"
                     /locus_tag="ACHOS5_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9779341.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3310..7053)
                     /locus_tag="ACHOS5_004642"
     CDS             complement(3310..7053)
                     /locus_tag="ACHOS5_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP9779342.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1634 a         2067 c         1889 g         1720 t
ORIGIN      
        1 gaaggttcga atccttcccc caccaccatc actttcaaaa gcccctgaat tctcaaacga
       61 atccgcaatt aaatattctg cccatgcggg gaaggatgag aggcttcgac caaggttcga
      121 ctcgagtgcc agcgagagag cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc
      181 gctgagtaat ccttccccca ccaccatcac ttcctgtaaa tactcaaaac ctgttactcc
      241 gagctaattc tgcccgatgg caccgcgttt accggaccta aaatatcctc ccctgaagtt
      301 cggataagcg aagcgcatcc gacaactcac cccgcaccgt gcccataaaa aaaccccgcc
      361 gaagcagggt ctatgtgaac taaaattcgc ttagtgacga gcgcgcacca gctgatactt
      421 acgcgtcaga tactccaccg ggacgctcca gatgtgtacc agacgcgaga acgggaacag
      481 caggaataac gtcatcccca acaccaggtg cagacggaag ataaacgcca cgccatcaag
      541 atgttgagaa gcgccaccgt ggaaggtcac taccgactgc gcccagccaa ccagtttcat
      601 catctcgcta ccgtccatat gctgggcgga gaacggaatg gtcagcaggc ccagcgcgca
      661 ctggataacg agcagcgaca ggatcaggat atccgctccg gtagtcgttg cacgcacgcg
      721 tgggctgaac agacgacgtt tcaacaacag cacgccgcca atcagacaca gtacgccgct
      781 ggcaccacca gcaaacatcg ccattttctg ttttacatca atcggcagcc aggcttcata
      841 catccagtgc ggcgtcagca taccgaagaa gtgaccgaca aaaatcccca gaataccgat
      901 atggaacagg ttcgacgcca gattcatccc tttgcgatcc agcatctggc tggacgccgc
      961 acgccatgtg tactgcccgt agtcataacg cagccagcta ccaatcagga agaccgcccc
     1021 ggctatgtac gggtagatat caaagaagaa catattcagg aattgcatta gtgctgtcct
     1081 ccggtggtga tattcagata ttgcggcgcg acggcaccgg caaagcgacg ctgatgagca
     1141 gtgattgctg aatcaccgca gcctttttca gcaaagaatt taacctgctc ttcttcccag
     1201 actgcatcca gcgcctgcgg cgtatcgtcg cgcgcttcat cggcaatttt ttccgccact
     1261 ttgtcgctgt cgattgcggt attcgccagt ttcagcagca gatcaaacag cacggcataa
     1321 cggctttcac gctgttgcag acgcgcgctc agcaatgcca gaatcggcgc aatatctttc
     1381 aaaccctcca cggcttcgct ttgcggcagc tgcgccaggt actccagata cagcggcaga
     1441 tggtcgggca attcgcggct gtttaactgc aagccgtgct gctcgtactg cgccagcaga
     1501 tccaccatcg cctggccgcg gtcacgggat tcaccgtgaa catgttcaaa tagcaacagt
     1561 gacgtggcac ggccacggtc aaacagttcg ctgtactggg cctgagcatc gagcggatcc
     1621 atcgccgtta aatcgcgcag gaaaatgccc agcgcatggg catcttcttt cgacagattt
     1681 ttcgacgcgg caatcgcctc aaacatctct tgttgatgct gccatgaggc agcatccgga
     1741 tattcaagga gacgcgatac aatcacgagt tcgatcatgg gtgcggctcc gttttgctgg
     1801 ttacgtcgat ggcatcgata cggcggctgt tgaacagatt gaatttggta tctgaaccgt
     1861 ggcagccatc accaaaggta aagccgcagc catttttctc cgggaaggct tcccgcgcca
     1921 gttcacgatg actactcggc accacaaagc gatcttcgta gttagcaatc gccagataac
     1981 ggtacatctc ctgtgcctgg gcttcggtca gaccgacctc ttccagcgca cgggtatcca
     2041 ctttcccgtc aacggtttca gcacgtttat aatgacgcat cgccagcata cgtttcagcg
     2101 cgcgcagtac cggtttggta tcaccggcgg tcagcagatt cgccagatac tgcaccggaa
     2161 tgcgcaggct ttcgacgtct ggcagaatgc cgttgctacc cagctcaccc gcgtctgctg
     2221 cagactgaat cggagacaga ggcggcacgt accagaccat cggcagtgtg cgatattctg
     2281 gatgcagcgg cagcgccagt ttccattcca tcgccatttt ataaactggc gactgctgtg
     2341 cagcctcaat aacgctcagc ggaataccgt ctttaatcgc ctgctcgatg actttcggat
     2401 cgttcggatc gaggaacaca tccagctgac gctggtagag atctttctcg ttctcggtac
     2461 ttgccgcacg ttcaatcgcg tcggcatcgt acagcagcac gccaagataa cggatacgac
     2521 cgacacaggt ttctgaacat attgtcggct ggccggcttc aatacgcgga tagcagaaga
     2581 tgcacttctc agacttaccg ctcttccagt tgaagtagat ttttttgtac gggcatccgg
     2641 tgatgcacat acgccagcca cggcatttat cctggtcgat caggacgatg ccatcttctt
     2701 cacgcttgta aatcgcaccg ctcgggcagg tcgccacaca tgccgggttc agacagtgtt
     2761 cgcacaggcg cggcaaatac atcatgaagg tgttttcgaa ctggctatac atcgccttct
     2821 ggatgttgtc gaagttcttg tctttcgcca gcttgtcaaa ctcaccgccc aggtcatctt
     2881 cccagttcgg ccctttttcg attttcgcca tccgttcgcc ggtaatcagc gaacgcggac
     2941 gggcaatcgg ctgtgatttg ctgccttccg gcgcggtatg caggttctga taatcaaaat
     3001 cgaacggctc gtaataatcg tcgatccccg gcagatgcgg gttagcgaag attttaccca
     3061 gcagcatggc acggttaccc atgcgcggct gcagtttgcc gttgatttta cggatccagc
     3121 cgcctttgta tttttcctgg ttttcccagt cagtcgggaa gccctggccc ggcttggttt
     3181 ccacgttgtt gaaccacgcg tattccacgc cttcacggct ggtccagacg tttttacagg
     3241 tcactgaaca ggtgtggcag ccgatgcact tatcgagatt cagcaccatg ccgacttgtg
     3301 aacgaatttt cattttacgc tctcctgtac ctggtcattg ccttcgccat ctaaccagtc
     3361 aatgttcttc atcttacgca ctacaacaaa ctcatcgcgg ttagaaccta cggtgccgta
     3421 gtagttaaag ccatatgcca gatgggcata gccgccgatc atatgcgtcg gtttcggcgt
     3481 gatacgggtg accgagttat ggataccacc acgctgttgg gtaatttccg aaccaggcag
     3541 gttaacgata cgttcctgcg cgtggtacat catggtcatc cctgccggaa cacgctggct
     3601 gacaaccgca cgggcagtca gtgcaccgtt gctgttgaag acttcaatcc agtcgttatc
     3661 ggcgataccc agctctttgg catcggcttc actcagccag accaccggac caccgcgacc
     3721 taaagtcagc atcagcaggt tgtcgctgta ggtggagtgg ataccccact tctggtgtgg
     3781 cgtcaggaag ttgagcgctt tttccgggtt gccattggat ttctggccca tcacttcttt
     3841 caccgaacgg gtgtcgatcg gcggacgata aaccagcagg ctttcaccga aatcacgcat
     3901 ccattggtga tcctgataca gctgctgacg gccagagagc gtacgccacg ggatcagctc
     3961 gtgaacgttg gtgtaaccgg cgttgtaaga aacgtgttca tcttcaaggc cagaccatgt
     4021 cgggctggag atgattttgc gcggctgtgc ctgaatatcg cggaagcgaa tcttctcgtc
     4081 ttctttattc agcgccagat gcgtatgatc acggccggta aactcgctca gggcagccca
     4141 ggcttttaca gctacctgac cattggtttc cggtgccagc gtcaggatca tctctgccgc
     4201 atcaattgcg gtattgagca tcggctggcc ttttgccgga ccttccgctt tggtgtagtt
     4261 gagcttacgc agcagatcca tctcgctctg ggtgttccag gcaatccctt taccgccgtt
     4321 accgattttc tccatcagcg ggccgataga ggtaaagcgt tcgtaagttg ctggataatc
     4381 gcgctctacg accataatgt gcggtgcagt tttacctggg atcaggtcgc attcgccttt
     4441 tttccagtct ttcacatcca gcggctgcgc cagttcagcg gcagagtcgt gctggatagg
     4501 cagcgtgacg atgtcggttt ctttacccag atggccgacg cacacttcgg agaatttctt
     4561 cgcgatggct ttgtagattt cccagtcgct tttcgcttcc caggccggat cgaccgccgc
     4621 agacagcggg tgaataaacg gatgcatatc cgaagtattc atgtcgtctt tttcgtacca
     4681 ggtcgccgtc ggcaaaatga tgtcggaata gagacaggtg ctcgacagac ggaagtccag
     4741 cgtcaccacc agatccagct tgccttccag accattgtcc tgccagtcca cttcttccgg
     4801 cttcacgccg ccctgctgcc ccagatcttt accctggata ccgtgctccg tccccagcag
     4861 gtacttgagc ataaactcat gacctttacc ggaagaaccg agcaggttag aacgccagat
     4921 gaacaggttg cgcgggtggt ttttaccatt ttctggttgt tctgccgcaa aacggatgga
     4981 accctctttc agggatttca ccgtatagtc caccggattc atcccggctt tttccgcttc
     5041 gccagcgata gtcagcgggt tagtgcctaa ctgcggtgca gacggcagcc agcccatgcg
     5101 ttccgcacgg acgttaaagt cgatcagatg tccggtataa cgggatttat ccgccatcgg
     5161 tgacagcaac tcttccgccg tgacggtttc ataacgccac tggctggagt ggttatagaa
     5221 ataagaagtg ctgttcatgt gacgcgccgg acgctgccag tcaagggcaa acgccagcgg
     5281 ctgccagccg gtttgcggac gcagtttttc ctgacctaca tagtgcgccc agccgccccc
     5341 gctctgaccg acacagccgc agaaaatcag catgttgatc agaccacgat agttcatatc
     5401 gaggtgatac cagtggttca gccccgcacc gacgataatc atcgaacgac cgtgggtttt
     5461 atcagcgtta tcggcaaatt cacgggcgat gcgaataatc tggctgcgag aaacgccagt
     5521 gatttgctcg gcccacgccg gagtgtaagc tttcacatcg tcatagctgg ttgcacagtt
     5581 aacgtcgttc aggccacgtt ccagaccata gtttgccagc gtcagatcat aaacggtggt
     5641 caccagtgcg gtgctgccat cagccagttg caagcgtttc accggcagct tgtgcagcag
     5701 cacgttttcc agttccactt tgttgaagtg ttccgtgccg tcgccaccaa agtacgggaa
     5761 gcccacctca gcgatctcat cctggctacc cagcagactc agttgcagct cggtttcttc
     5821 gccagttttg ccgtcgcgct gctcaagatt ccatttgccc ttctcgcccc agcggaagcc
     5881 aatagaaccg ttcggcgcaa ccatttcgcc attggtatta aaggcgacag ttttccattc
     5941 cggattgttt tcctggccca gcgcatccac cagatcggca gcgcgcagca tacgacctgc
     6001 ggcgtagtaa ccgtcgcgtt cttccagcat caccagcatc ggcatgtcgg tgtagcgacg
     6061 cacatagtcg gtgaaatact ggcttgggtt gtcgaggtga aattcacgca gcattacgtg
     6121 gcccatcgcc agcgccattg ccgcatcggt gccctgtttc ggtgccagcc acagatcgca
     6181 cagtttggcg atttcagcgt agtctggtgt gacggcaaca gttttggtgc ctttgtaacg
     6241 cacttcagta aagaagtgag catccggggt acgcgtctgc ggcacgtttg aaccccaggc
     6301 gatgatgtag ctggagttgt accagtcagc agattccggt acgtcagttt gctcgcccca
     6361 ggtttgcgga gacgcaggag gcaggtcgca gtaccagtcg tagaagctta agcaagtacc
     6421 gccaatcagc gaaagatagc gtgcacccga tgcgtaagaa accatcgaca ttgccggaat
     6481 tggcgagaaa ccagcgacac ggtccgggcc gtagtttttg atggtgtaaa cgttagaggc
     6541 ggcgatcagt tcgttcactt cctgccagga agaacgaaca aaaccaccgc gtccacgcgc
     6601 ctgcttaaag cttttcgctt tatcggcgtc ttcaatgata gaagcccatg cctcaaccgg
     6661 atcgctatgc agcgccttcg cttcacgcca cattttcatc aggcgtttgc gcatcatcgg
     6721 gtatttcagg cggttggcgc tgtaaagata ccaggagtag ctggcaccgc gcgggcaacc
     6781 gcgaggttca tggtttggca ggtccgggcg agtacgcgga tagtcagtct gctgggtttc
     6841 ccaggtcacc agaccgtttt tgacgtagat tttccagctg caggagccgg tgcagtttac
     6901 cccgtgggta gagcggacga ttttgtcatg ctgccaacgc tggcgatatc catcctccca
     6961 gtcacggttg gtattgagaa gctggccatg cccatcggca aaggtttcac ccttctgttt
     7021 gaagtagcga aaccggtcca ggaatttact catcggtttt ctcctgtggg agcctgtcgg
     7081 ccctctgata aatcgacatt gctaatgttt aagggcaagg taacgctctg aaacagcgtg
     7141 agaattgata acgatcaaga gtgatgggga tgaaaaataa agtaaattcc taagataccc
     7201 ctttaggata attagcagtt acagcataac acgctgtaaa ttaagacgtt ttatacctgt
     7261 agttatttaa gctattaaga tttttatgca gatgggtatt aaggagtatt
//
LOCUS       DAZKHK010000188         7172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKHK010000188 DAZKHK010000000
VERSION     DAZKHK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.189"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(5..3124)
                     /gene="agn43"
                     /locus_tag="ACHOS5_004643"
                     /gene_synonym="flu"
     CDS             complement(5..3124)
                     /gene="agn43"
                     /locus_tag="ACHOS5_004643"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP9779343.1"
                     /translation="MKRHLNTSYRLVWNHITGAFVVASELARARGKRAGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDDNTGGQ
                     QIARGGTTRNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNGGEQWIHA
                     GGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSTGQMVGGIAESTTINK
                     NGRQVIWSSGIARDTLIYTGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGWQ
                     VVKAGGTAGNTTINQNGELRVHAGGEASDVTQNTGGALVTSTAATVTGTNRLGAFSVV
                     EGKADNVVLENGGRLDVLSGHTATRTLVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLAG
                     TTTLNNGATFTLAGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDIIAHRGTALKLTGCTVLNGTIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPNMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGMATTGKGIQVVEAINGATTEEGAFVQGNMLQ
                     AGAFNYSLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGTTPESSGSYGFVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGITFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGYN
                     SQATLNVTF"
     gene            complement(3455..4327)
                     /locus_tag="ACHOS5_004644"
     CDS             complement(3455..4327)
                     /locus_tag="ACHOS5_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9779344.1"
                     /translation="MNPSDAIEAIEKPLFSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIKPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            4628..4735
                     /locus_tag="ACHOS5_004645"
                     /pseudo
     CDS             4628..4735
                     /locus_tag="ACHOS5_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            5215..5448
                     /locus_tag="ACHOS5_004646"
     CDS             5215..5448
                     /locus_tag="ACHOS5_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779345.1"
                     /translation="MSQMPLYFLNTQKKLTAHYEWLQINLTDTYELVKRLMPIPSLDV
                     VVKVGKLVLPEKGHHGFYPEAGVVYRTVAPENP"
     gene            5492..5791
                     /locus_tag="ACHOS5_004647"
     CDS             5492..5791
                     /locus_tag="ACHOS5_004647"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HFP9779346.1"
                     /translation="MNCIVLLDGPRYGSTLGEVIVSEGLAGHFSLELFAGEPDPREKL
                     TSDIVQPYRLHLLENWYSTDYDCNAWLFGTGDLPRWLEYTKGFKLISRYLVHSHI"
     gene            6000..6359
                     /locus_tag="ACHOS5_004648"
     CDS             6000..6359
                     /locus_tag="ACHOS5_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779347.1"
                     /translation="MTFRYYLRHCLWGLVGCGSLVYNILTDLKDGKIFPSYIPYIPYV
                     AAYLAIGAVAYPFAYYALEISAQKIIPKKSWDRFFSDTGSSRNLFLFVLMFCILFIVP
                     LLALYFCISKKHNKLQT"
     gene            complement(6864..>7172)
                     /locus_tag="ACHOS5_004649"
     CDS             complement(6864..>7172)
                     /locus_tag="ACHOS5_004649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779348.1"
                     /translation="HWGRSKNKRVNSEHPYILPMSGKINYGLDYSPLFMFLLRKTGEK
                     SDQVYSEAILRLDLPDPTFLLVALHETDQRNFVRCGKSSYYNGLYVDENGYLKCFLQI
                     "
BASE COUNT         1778 a         1819 c         1730 g         1845 t
ORIGIN      
        1 attatcagaa ggtcacattc agtgtggcct gactgttata accttcagcg ctgctgccgt
       61 tgatgctgtg ggcataaccg gcctgaacgc ccagggtgat attttcccgg acacgggctt
      121 ccagtccggc ctgcagatcc agtgaagtgc cattctgtga gggagagaac gttatcccgc
      181 tgcctgcggt ggaggtcccc acacgcatat caccccggga gctgaaggtg cggataacgg
      241 aaggctgtac ccaccagttc accggcagtt cacgcacact gtgttttgca ctgtcacgca
      301 ggggggcacg ggatgaggtg ccttcaccaa aggtcatatc gttgtggctg cccagacgga
      361 aaccggcacg cacatgttgt gcactgccat gcccgaactt cacataaccg gcgttgtcct
      421 taccgtcatc cagggagagc ccctgccagg tgtactgcag tcgtggctcc agcatcaggt
      481 tgtcagtgat actgaagggc agaccggttt ccagtgaacc cagccagccc cggccccggg
      541 cgcggaagtc gttattgtcc gatgatgctt tcatgctgtg gcgggttccc tgtgccacaa
      601 tgtcagccca caggccggag gatgtatgtg tcagattcag gtatccgcca aggctgccgg
      661 catcatcccg gaccgtgccg gcgcgggagc cgtcatcatc cttaacatct acggaagaat
      721 ggcctgcagc accatatact cccgcggtca cagacatacc ggcaacctct gttctcagca
      781 ggtcaccctc caggcggacg aagccatagc tgccgctgct ttccggcgtg gtcccacggg
      841 caataccgcc gttgttatcg tgaccgagat ggccgccctg aatgctgaga cggacgctgt
      901 tattttcacc gtttacaccg gtctgatggc tgcgtgagcc tgccagaatc cggtcatagt
      961 ccattgcctg tgtcagcatg gaggcgtaca gggggacttc agcacgataa cgttcttcac
     1021 tgcgcagata ccagctctca tcactgtccc ggttgaggga gtagttaaag gccccggcct
     1081 gcagcatatt cccctggaca aaggcccctt cctccgtggt ggcaccgtta atggcttcaa
     1141 ccacctgaat acccttaccg gtggtcgcca tccctgacgc actgttgccg gcgttcacca
     1201 ggttcaggat ggtttttccg gttgccctgc caccgtcaat gaccagtctg tcagcattgt
     1261 tctgtgccat attcgggcgt acacgcaggc tgatggtgcc attctgtccg ttcaggtttt
     1321 tcactttcag ggttgccggt acgaacttcc ctgtgcgggt ggaggtgaaa tgaatctgtc
     1381 cggcatggct gaggtcatcc accaccgact gaaccgtggc gttatcggga atattccagg
     1441 tggcacctga ggcgagggtg acattcgtgg ggtcaatggt accgttcagc acggtgcagc
     1501 cggtcagctt cagggccgtg ccgcgatgag cgatgatatc cgtggtgacg gtactgtcgt
     1561 tcagcgtcag cgtgccttca ttcaggttga cggctttctg ggtgagtgtg gtgttgctga
     1621 cagtgagtgt gccacttcct gatttttcca ccctgccgtt accggtgaga gcgcctccct
     1681 gcaggagtgc atcaccttca cggatggtca gggtatcgtt gttcaccgtc ttcccggcaa
     1741 gggtaaacgt ggcaccgtta ttcagcgtgg tggtgcccgc cagcgagccc cctctggcag
     1801 tgaacagtcc gccatttacc gtggtatccg tggccgtatc accggcgttc agcgtgaatg
     1861 aactgccttt ttccagcatc agggcatccg cctgaccgcc cccgatacgg aatgccgttc
     1921 cgtcgctccg ggtaccactg acagcggccc cggaatcggc cagcagtacc ccgccatttc
     1981 ccatggatac ggtagtggcg gtaccaccgt tgcggacatc cagcgttccg ccatcatcca
     2041 caagggttct ggtggctgtg tgtccgctca gcacatccag acgtcctcca ttttccagta
     2101 cgacattatc agctttaccc tccacaacag agaatgctcc caggcggttt gtaccggtga
     2161 cagttgcagc agtgctggta accagtgcac ctcccgtgtt ctgagtgaca tcagacgctt
     2221 caccgccggc atgaaccctc agctcgccgt tctgatttat ggtggtgtta ccggcagtac
     2281 caccggcctt aacaacctgc cagcccccct cgttaattac cgtgttcagt gcaagtccat
     2341 acttgtgcac atactggttc cctccctcca gtcgggtgtt atgtgcctct ccgtgtaccg
     2401 tctggtcacc tcctgtgtaa atgagagtgt cccgggctat ccccgaagac cagataacct
     2461 gccggccatt tttgtttatg gtggtggatt cagcaatccc cccgaccatc tgaccggtgg
     2521 acacattttc agactccgga ccaccttctg caccggtgtt gacgatggtt ccggttgcct
     2581 gcccgccatg tttaatggtc tggtagccag actggttaat caccgttccg gatgcactgc
     2641 cgcctgcatg tatccattgt tcaccaccgt taaggacagt accgtaagcc tgcccgcgca
     2701 gattctgtcc tccaccggca ctgataaccg tatccgtggc actgccacct gccatgacat
     2761 cctgcagacc gtttgcggtg accgtggtgt ttctggtagt gccaccccgg gctatctgct
     2821 gaccgccggt gttgtcatca gagtccggcc ccagctcaag cccggtactg acagtcacgc
     2881 catcagctgt tccggataca aactggttgt catggtttac cagtgttccg ccattcactg
     2941 tttcacccgg gtgcacaacg atgtcagcag ccagcaccgg gagtgacgtg actgcggcaa
     3001 gagacagtgc aaccgccaca ccggcgcgtt tcccccgtgc acgggccagt tcggaggcaa
     3061 ccacgaaagc gcctgtaatg tgattccata ccagcctgta gctggtattc agatgtcgtt
     3121 tcatcagcat ttccttatca gagtgagggt gaataaaaag ccggtatcca caaatgtgga
     3181 taccggcagg tgaagggtta cagtgccgca gatccggctg atggaagttt ctgacgtgtg
     3241 gctctgttgt tccctgaatc tcatgtctgt gtgtctgcat acgttgctaa cgggtgttat
     3301 tattagcata acgatcgata taaacgatcg attaaatagg tatcgatcgt tttattctat
     3361 tacctgattg ttgcacggac aagaaaggtg gcaatgaaaa ccgcgctttt tgtgagatgg
     3421 ctcagaggca gggagggcat catctacaac aggttcagaa gaaaatccag ttccataccg
     3481 cgcgggcaac agagaccacg gtgtcacgaa cagcgcgcag ggccgtacgg acaacagagg
     3541 caacacagac gctctccgct gtgtcaaata tccggtccac cgcaccggta aactgttcac
     3601 gggcctgaga gcggacagac tccgtgcgca gcccgtcctg caggcgggtc atcaggggac
     3661 tggcggcatg gtcgggaagc gctgtcatga gcgcactgac cagcgtatcc agctcccagc
     3721 cggtgcgggc cgatacggca acaaccggat gtacgggccg gaacagacgg aataccgcct
     3781 ccgttttttc gcgaatattc tgtgcctgtg cgggagaagg cttaataccg gccatatccc
     3841 attcatggca gggctccgtt ttatcggcct gcgtcaccac aaacagtacc cgctgatgtc
     3901 cccggtgcag gatgtgtcgc cagaaatact catccacaga cagggcacgg tcatcggctt
     3961 taatcagcca cagtaccagg tccagttctg gcagaatgtc acggtacagg gcttcatact
     4021 ctgcatccct gtcccggctc tcgcccaccc cgggcaggtc agtgataacc atgctgtgac
     4081 cgtggccact cagacggaag cgccgcactt cccgggtgcc agcgtgaaca tcactgaccg
     4141 gggtgacctc cccctgaaac agtgcattac agagtgagga tttaccggcc ccgcttttcc
     4201 ccataatgcc aatcacgggt tcgtggctgg tgagtttgcg cagatgttcc aggatgtgac
     4261 gggaaagtga ataaggcagg gagaagagcg gtttttcaat tgcctcaatg gcatcagacg
     4321 gattcataca tgtattcccg gtaaaaaaag acaaaacccc gcgcaccgga aaggtggcgg
     4381 ggggattcac ggagataata tctgtctgaa attatttagg atgtgccaga catacagcaa
     4441 aggagagaag tattcggcca cgcctccctg ccatgaataa cccggagtcc accgacttac
     4501 ttacgggcta cctgagccac gcgtttgctg tagaatagcc cctgatgatt catacctgaa
     4561 ccaggaaaac gaattaaaaa tgacaggcat tcaataacga gcagggctgc aacaccagat
     4621 agggcaaatg gtcaatgatg tcagagggtc tgtcgacgga tgggatttta aacagtacat
     4681 tttaggcgca cttttcaacc gttttatcag cgaaaatttt tccagccata tggaatattg
     4741 tcgatcaaga tgttgtaaaa agatccagaa aaaatatttc aaaggcatga aagaacttgc
     4801 tgacaagaag tttattgcag agaccatgat acagaactat tactttatta acccaggcta
     4861 tatgtttaat ggtcatcgct tgacctatat gaagacctat tacctgaagg ataaaaacga
     4921 ataaataata atagagagat tcctgtgaag taatttcata ttaattatca aatggtaact
     4981 aactatttaa taatacatgg cgaatggact gttttaatta atatattagc tcagacttaa
     5041 cctgagacag ctatgacaca aaagccagag ctattttgat ggtttgagtt tgctctgccc
     5101 taagagcagg tatgatgggt aacagtctgg tcctctacta ctgtgcttac accacaacat
     5161 ttacgttgtg tgttaacatg tagcgattta ctgactcttt tgagtggtac cgttatgtct
     5221 caaatgcccc tctattttct taatacccaa aagaaactca ctgctcacta tgaatggctt
     5281 caaatcaacc tgactgatac ctacgaacta gttaaaaggt taatgccgat tccttcactg
     5341 gacgtggtgg ttaaagtagg gaaacttgtc ctcccggaga aagggcatca tggtttttac
     5401 cctgaagctg gagttgtcta tagaacagta gctccagaaa atccataatt ttgcaaaaat
     5461 tatgcgcact ccattgctcg aatgtttgca catgaactgc atcgtgctgt tagatgggcc
     5521 gagatacggc tcaactttag gagaagtcat agtctctgaa ggacttgcag ggcacttttc
     5581 gcttgagctt tttgctggtg aacctgaccc cagagaaaaa ctcacatcag atattgttca
     5641 accgtacagg ctgcatttgc ttgaaaactg gtatagtact gattacgatt gtaacgcttg
     5701 gttgttcgga accggggact tgccaagatg gctggaatat accaaaggtt ttaaactgat
     5761 ttccagatac ctcgtacatt cccacattta aaggcttcaa tgctggcgaa tataaacact
     5821 gaggagttaa agacatttat ttaaagagca cagccatttt ccagaactat gactgtgatc
     5881 atgggcaaaa cccaaatggc ccactcatag cggcctatca gagttaatgg ctttcagttt
     5941 gcggaaactg cctacttgaa tccatatgta ccctatttca tatcattaag gtatataata
     6001 tgacatttag atactatctc agacattgtt tatggggctt agtcggttgt ggctctttgg
     6061 tttacaacat tttgactgat ttaaaggatg gaaagatatt tccttcttat attccttata
     6121 taccttatgt tgcagcatac cttgctattg gtgctgtagc atacccgttt gcatattatg
     6181 ctttagaaat atcagcgcaa aagataatac caaagaaatc ctgggataga ttttttagtg
     6241 atactggatc atcaaggaat ttattcttat tcgtattaat gttttgtata ttatttatcg
     6301 tacctctact cgcattatat ttctgcatat caaaaaaaca taataaatta caaacctaaa
     6361 attacattta attatgctcc ctaaaaacag tttaagttat tatcaataaa gttagcaatc
     6421 ttcttaatac ctatttttac atacccgaag ctaaaaatct aggttcagct aagtataact
     6481 ctattggacg gtaaacctga acgggctgac aacagggaca atgaaggtta actgtgacca
     6541 tggaagaacc ttaaaagcga ctattatacc cctaattcaa ctaattggag gtattaccat
     6601 ctattgatac tgataattct aaaaaggcat ggacagctca gggccaaact ccgaaaacaa
     6661 caatagctac tatcggatct gttaaaacat tggtaaattt tgcataatta taaatacaat
     6721 gtgtttaacg ccctgaaata aaacaattga tgcgtctatt ctttaagtta ataaggtcag
     6781 aaaaatttca acttattcat ttaataattt cccgtttaga gtgtaagtac agcacttaca
     6841 taatattgat atgcctgatg cctttaaatc tggagaaaac atttcaagta accattctca
     6901 tcaacataca gcccattgta ataactggat ttgccgcacc gaacaaaatt tctttggtct
     6961 gtttcgtgta gagcaactag caagaaggtt ggatcaggca gatcgagtcg tagaatcgct
     7021 tcactataaa cttgatccga tttttcacct gtttttctta gtagaaacat gaataatggt
     7081 gaataatcaa gcccatagtt tattttacct gacataggta aaatgtatgg atgctcagaa
     7141 ttaactcttt tattcttact ccgcccccaa tg
//
LOCUS       DAZKHK010000189         7154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZKHK010000189 DAZKHK010000000
VERSION     DAZKHK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.190"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(49..1068)
                     /locus_tag="ACHOS5_004650"
     CDS             complement(49..1068)
                     /locus_tag="ACHOS5_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9779349.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWVRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKIRLLGISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            complement(1210..1401)
                     /locus_tag="ACHOS5_004651"
     CDS             complement(1210..1401)
                     /locus_tag="ACHOS5_004651"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9779350.1"
                     /translation="MCKRLLAIPGFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            complement(1814..2029)
                     /locus_tag="ACHOS5_004652"
     CDS             complement(1814..2029)
                     /locus_tag="ACHOS5_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9779351.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            complement(2250..2507)
                     /locus_tag="ACHOS5_004653"
     CDS             complement(2250..2507)
                     /locus_tag="ACHOS5_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779352.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTHKGT"
     gene            complement(2576..4273)
                     /locus_tag="ACHOS5_004654"
     CDS             complement(2576..4273)
                     /locus_tag="ACHOS5_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP9779353.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            complement(4369..5505)
                     /locus_tag="ACHOS5_004655"
                     /pseudo
     CDS             complement(4369..5505)
                     /locus_tag="ACHOS5_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            6181..6389
                     /locus_tag="ACHOS5_004656"
                     /pseudo
     CDS             6181..6389
                     /locus_tag="ACHOS5_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            6376..6565
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004657"
                     /pseudo
     CDS             6376..6565
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(6568..6756)
                     /locus_tag="ACHOS5_004658"
     CDS             complement(6568..6756)
                     /locus_tag="ACHOS5_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9779354.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
BASE COUNT         1875 a         1638 c         1452 g         2189 t
ORIGIN      
        1 tcagtattca tcctttcatt aaaagttaat atttatcggt atcagagatt aatatgatct
       61 gatactgaca tgattcttgt cgtatttact tccatgagct gctatcgccc atactgttcg
      121 tgccagtttg tttgccatgg cgacgattgc cacgctggtt ggacgacgtt ttttcagttc
      181 ggttatccac ggaccaggtt ctttgggcag taatgctgcc gcccttgcgc tatgaataaa
      241 caaggctcgc agatatgtat taccacgttt gcttatcccc agcaggcgga ttttccctcc
      301 tgagccagtc tgtttgggca ccagaccaac ataagccgcg aactctcgcc ctgatttaaa
      361 cgctgatgct tcccccatgg tggccaccgc tgccgttgca ataagtggcc caacgccggg
      421 aatatccagc aaccgcttac aggtttcatt ctgtctcgcc acgctggtaa gctttttttc
      481 aatatcctct atcagcgagt ccagctcatt cagccggttg tactggtttt ccagcactgt
      541 aatgagatac ggcggcagtc tctctttcat ccgctccagt gcttcaggga gctctcgctc
      601 catcgctgcc cgacctttgt gaatggtttt accaaattct aggagcgtgc cgtgcagaga
      661 attaatctgc atggtgcgga acttaaccaa ttgtctgcgg gaacggtgca aaaccagcac
      721 tgactgttgt tcttctgttt taaccgcgat gtccttacct ggctgctgaa cagccatcca
      781 gatagcccgg gcatccatca catcattctt atttcccatg acgaatgcct taacaaagtg
      841 ggcctgtaac aaccggactt tatgccccag ctttgtcagt tcacgcaccc agtgctgaga
      901 gcctctacag gcctccatac caatcaggca gggttcccga ttacagaaga acatcagaaa
      961 atcctgacga cgtagttgct tatcaatcac ttcacctgtg tgctcgtcga tgaaatgaac
     1021 ctgaatcaga tgttttgcga tatcaacgcc aaccggtgta tatttcattt gtggagtctc
     1081 cagtctgagg gagctttatg catcctgtat tgggcactat gatgccggaa atctgcgagg
     1141 ctccacacct tccagtattt cccttcatcc atcacagagc acctgactta atggggtggg
     1201 atgtgttcat tacatttctc caaattttgt tatacccaga agaacctgct tcccacctgt
     1261 gctgaactgt cggggaacca gtcccagagt cgcagatgca tctcttcctc gtctaaactg
     1321 ttttccatcc cccatccagc tttttattgc ctgactaacc agagggctga atccaggaat
     1381 ggctaacaag cgtttgcaca taggatcttg gcggacatat ttttcagttc agaatcatac
     1441 cactccagtt cattatctag tgtaatcaac cgtatatact gccgatgcaa taacgtcctc
     1501 atggtatcag tgagttcgtt ttccgcatcc tcaagtaatg ccggtaattt ttggcgtaat
     1561 tctgttgcgc ctttactgaa cacaatgcca tattcagcaa gaagtccacg aacgtgattt
     1621 atcagttgtg tcctttccat cgaaaccaac cggcgcattt tcaaaaaagt ctggacgtcc
     1681 tgttactcca gagtttttat tggtacgggg cgtatagttc catgttggca ggcctctgct
     1741 attgcctgag cgtcgtcata atcatttttc tggcgtcgct gataagcttt aacatgttgc
     1801 gccggaagaa atattacatc atggtcgagt ttcctgattt ccctggccca gtgatgggat
     1861 gcccgcgagc ctccatcgca acagtcattt tgggctgttg caccagccag ttaagcagtt
     1921 gattgcgaga aagtttgatc cttcctgcgg gtttgccatg agcattgata ctgaaaagat
     1981 aaaagttcgt tttagccaga tcgatgccgg tgattttctg ttgagacatg gtatccctct
     2041 tacttttgtg gttaaagttt atccatcatg acgtaattaa aggttgtcgt gaaggagggg
     2101 gaagaccatc tcatcgctta ctgatgttcc tgttactata tctattgaat cctggctata
     2161 agttcgctgt ttaaagaatt atatgaatag caccaattaa taagttaata ttaaaaagaa
     2221 gacagacctt ctaaataaac gcattgaatc tatgttccct tgtgagtgtt tttacgtttg
     2281 gcgggaggcc tggaacctcg ggatttaact tttcttttta tatttttgat aaaatcatca
     2341 atagaaaact gaatcgtctc tgataaatac tgttgagcta tatctgtact ctgtttttct
     2401 aaatcagaag ctttctttgt ttcaagaaca gatttgatta actccttacg ttgtgtacac
     2461 caaatctcgt cagatgaaca gtatttttta ttttcgtgtt tgttcattat ttattgctct
     2521 aactgattta tttccctata tccagggagt agatatatct actccctgct tattatcatt
     2581 tcacgttagt taaagatttt tttatattct caaatttggc ttggttgata tcactgatcg
     2641 gtttttgact tgacgtgatg aagtcaatca taacagcctt catattctta actacatcag
     2701 gatgggatgc agagagatct ttagtttgtt gcagatcgtt gatatcgtac agagctagtt
     2761 tattctctgc ataggtgtaa accagagaat aatcgttgct tcttacagtg tatgcaaact
     2821 ggcttaagtc ttctgtatgt gggttgtgcg gataatcatc agactctttt ctcacgaatt
     2881 tatgatatcc gttccagaat gcagtgttct cttcgtccca ccagtgagaa taagaggtaa
     2941 tccaggacag atatttgtga ggttcgccag ctgcagattt cttatcagct cctttctgta
     3001 catatggcaa taatgataca ccatccagtt tcagagaagc agggatcttg atatctgcgg
     3061 catctaaagc ggtcggatag aagtccattg atgaaaccag tttgtcataa tcacccgttt
     3121 tcagcttgcc attccaccac atgaacatag gtgtatgcat accacccgga tacggttggc
     3181 ctttgtaacc agcctgcgca ccattcagtg gcagtggacc atcaattaca gcaccgttat
     3241 cagaggtgaa cagaatgatg gtgttgtcgt actgaccttt ttccttaagt gtattcagga
     3301 tctttttaac cccctggtca acggagtaaa ttgacgcata atagttatca gcagtcttgc
     3361 taccagtgtt gaatttctcc tgatagtttt taggcgcagg ctgatcgtta ggcaggtgtg
     3421 gtgcactgta tgccagataa atcataaatg gctgttccag tttgcttgca cggtcaatta
     3481 caccaattgc ttcatcagtt aattgatcac taatgtaccc ttcagcttta atgttttcac
     3541 ggttacggaa caatgacggg gagttataaa gtgaaacgcc ggatgcgtga tagcccatga
     3601 agtagttgaa gccacgattc tgaggttgcc attcttctgc ggagaaggtg gtgaagttat
     3661 cgtggtagtc acgggtttgt ttttcttcag gcacacctac gttgctgatt tttgataagt
     3721 gccacttacc gattgctgca gtgtaataac catgattctg aagtaattca gacaggaatg
     3781 tttcttccag agggatacca tcctgtgcat cagaatttga atatacaccg aagcgagcag
     3841 gagctcgcgc cgtcaggatt gcggcacgtg aagggccaga gacaccgtgt gcaacatagc
     3901 cgttagtgaa ttttacacct tcgtccatca gggccgctaa tgttggagtg gatttttttg
     3961 cagcttcaat tgctttatca atacctattt tgtatgtatc aacaacattt cggttatcca
     4021 tcgttttagg gtcaaatgaa ctatcatcaa aaggcaattg tccataccct aaatcatcca
     4081 ttgttaaaac aatgatattt ggcttacctt tagtgctgta ttccgtcggc gtgaagtccg
     4141 caaaggcaac attggttttt gttgctttga gcttaacatc atctgctgca tttgcagtac
     4201 tcatcatcag accagatgct aatataagtg atattgatga gttaagcagt tttttattta
     4261 actgatgttc catatggtat tccttctgag ttgcatcacg aacatttatt aataataaaa
     4321 gcctctgata taaataaaaa ctatgtaaat cgtttaaatg gatacctgtt aaaactgata
     4381 catcatacca acagcaagta tattatcggt tgcaactcca gcctttttgg taaattcgct
     4441 ttcatcaata aggttaattt tgtaatcaat aagaagtgac atgtttttgt tcagaaagta
     4501 gtttgcgcca acatcaatat actcaacaag agactgatcg ccatagttta ctattccacc
     4561 tttaacatcc atccctttgg actgaacgta agccagtgaa ggacgcaggc cgaaatcaaa
     4621 catatactga gccacaactt caatattccg tgctttatct gcgatgcctg cgctaccaaa
     4681 tggagtcata tttcgggttt cagagtagtc agctgccaga tacaggtcat tatcattata
     4741 cttgacccca acacctgcaa actccgcatg ttttcccccg gcaatccagc gaggcgtaat
     4801 aacttctttt ccttctgaat ctttcgatgt catgaaacgt ttacggcctt ctttttcctg
     4861 cagactggtt ctgtcggcat tagtgtaagc ggcaacgatt ccaaaacctt catagtcata
     4921 gcttacggag aaaccatatc catcaccatt agcttcaagt aaattatcac ggtcattttt
     4981 accctgatat tgagcagaaa aagtaagtcc atcaacaagc ccgaagaaat ctgtattgcg
     5041 ctaggtcaga actccggttg ttctgccggt catatagtta tcagagtaag tccagctgtc
     5101 accaccaaag tccggaagta cgtcagtcag agcccccaca tcataggcaa caccatagtt
     5161 tcgcccataa tcaagtgaac cgagtatact gtggcgaata cctgcaaatg ccagacgatt
     5221 tttatcgttg cgtacttcac tttctgtctt aaaccccagg aactggtatt cccactgacc
     5281 aaagccagtc aggctctcat taatttgtgt ttcgcctttg aaaccaagac gtgcaaatgt
     5341 tctgtcgcca tccatagaac catcagagat gtaatgacgg gctttaacgc gaccataaaa
     5401 atccagttta ttaccgtctt tattatagat ttctgctgcc tgtgttgcct cacacagcat
     5461 taatgcagaa acgacagagc acacaaccat acttaatttt cgcataaaaa aatcccctgt
     5521 gcacattttt agtatggttt taaaaattaa actatggcga aaaaattatc actatgcaaa
     5581 gatgtaacaa aatattgctc agtgatctat gttgttgttt ttatttgagg ttgtatattt
     5641 tctagtgtgt ttgacttttc tttctcaagt gttatttctt gacctttatc acagaaccat
     5701 ctccctggat atccccatca cattgcatga aaagcaagtt ttaccaatct tgattttcag
     5761 tgttaaataa gattaataac tgtaattctg caaaaaaaag aacaccatat gtagtatgac
     5821 tacgtattcc actctgttcg ttcacggact ggcatcgaag tctgaggtgt actggcaata
     5881 gcggatacag tcatttgttc tttttttaag cattcatccg atgatacttt tcctgaatgc
     5941 taccggagtg gtattcccct gccgtgaggg agggcgctga cggttataaa aatcctgata
     6001 tatacccatt cccgtatgac agcgatggcg tcacatctct gttctaccat cttcacagaa
     6061 atatttcgtt cattagcatt ccggtattcg tataaccacc ttcggtggag aaagaataaa
     6121 caatccctgg aatgcgatga gcgcttgacc agaaaagcat catattgctt catggcgcat
     6181 cagcgaccac aggatgccgc aaaagccgaa aaacggacca gagcgcctgc aatcattcct
     6241 cgcctcagta tctgttgccc atggagtggc cgataacaga tcattgtcgg atgagcctta
     6301 tccccttcct tcccggtggt atccggatgt gctgccagcc ttctcagtta accgatgttt
     6361 tccgggcttt ttgccatact gaggactgat aacgtattcc tggtcgtaaa acccgtcaat
     6421 atgcgccggg gcatcgacaa aactgacaca gtacgtccgg aaagaactta acgctgcatg
     6481 gcacgacaat gctgcctttg tctttaccaa caaggctcgt tcccgcataa aagttctacg
     6541 ccgggataaa catggcgtat ggctgcgtca ggccttcact acttcacacc ggaagtcttc
     6601 caccctgaca cgaatatacc gtccgggctc tttcgcaggt gcaaacagtt tttcacattc
     6661 ggcgaatgtc atttctgaat cctggtaacg caaccactga accggcatat taccgggttt
     6721 gattattgtc gcagttattt tatatcgttt ccccatatcg cctctcatcc tgacaacaaa
     6781 attaccggct gagccatcac tccggtaaag catgcgaaaa aaagtgagcg tctcagcaat
     6841 ggctcatgcg atgattctgt acgggcaccg atggcacctc agaatacaac aaagatatca
     6901 gagtaacatg tctctctcgt tatttttcag agttcttgac accctccttc tgccacattg
     6961 tcaacgccag tggagacttc tgctgtcagt tttttcatcc atacagagtg acattaatta
     7021 tcctgaacaa aacgaaatat cctgaatata tttattttct cctggtgccg gagacatgat
     7081 gatgtacagg ctcaggtgcg gtgtgaatgt aaaatggtat gttaattatt catttctgaa
     7141 tgcaaactga atgc
//
LOCUS       DAZKHK010000190         7106 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKHK010000190 DAZKHK010000000
VERSION     DAZKHK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.191"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3365)
                     /locus_tag="ACHOS5_004659"
     CDS             complement(<1..3365)
                     /locus_tag="ACHOS5_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9779355.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLAASVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALADMLFPPQTEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEEARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVAGAAPGST
                     LTPDDKIDCHKHPSSLTQLLAQKKAAFLDDPPGTLLNLLSPDGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPAARSGARCTCEARVVNAPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSEIACFAAGIGMSILTQKAATA
                     LPHPVSAATGAKYLAGDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVRTPGSADENCMTFVSGMGRRLEMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QFFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRITEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPDTTPLLLRRYRYDGAGDLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWSLAARRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDAQGGKWRYVYDRQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNSWRCVYDERGNLLAVTDPLQQNTRYQYDRHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAESHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDATGRRTAF
                     GYDAYGRLVTLTNENGESYRFRHDVLDRLAEQINPDGCRQAYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETAGSRTEYVHDAADQLLEIRRRRPDAGETDAPEIIRF
                     SYDRLGRMLSEETAQGILTHQYDELSNRTATTFPDGRTQRHLYYGSGHLQQINLDREV
                     ISEFTRDHLHREVLRSQGRLSTRQLYDPGGRLKR"
     gene            complement(3384..3836)
                     /locus_tag="ACHOS5_004660"
     CDS             complement(3384..3836)
                     /locus_tag="ACHOS5_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP9779356.1"
                     /translation="MTEYYLNETVVTFPGNIIQDSTINMLRLSDPDAALIISRGQMQE
                     GDDLTSQIEQQMKKLEKQVKDLHYTPVQVTRVGINDGEEGLEIQSQFLRGNEQVYQCQ
                     VAFVLPGERVMMAFTYARTTPLTLADMTRWAEIKKNLRFRMRQEAGTN"
     gene            complement(3887..5881)
                     /locus_tag="ACHOS5_004661"
     CDS             complement(3887..5881)
                     /locus_tag="ACHOS5_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9779357.1"
                     /translation="MSFAATDYPSGALAGSGTHNVTLSGSAVPDGMLLFASLGGTETV
                     GELFTYSIKLKTPDILNLDYVSPAANLQLKPMVGKDLCVNIELDGGGKRYISGLVTAA
                     RVVGHQGRSVVYELRLEPWLKTLTHTSDYKAFQNKNVVDILDEVLDEYPWPVEKRLVE
                     NYPTRAWQVQYGETDFDFIQRLMQEWGIYWWFEHNENSHTLVLADAINVHKACADSPL
                     VCYYQKGLKLDKEFIHTITANESLRSGQWVLNDFDFMKPRSLLKSTVANPRETGLAEY
                     EHYEWPGDYFTKSEGEMLTRIRMEEQRSPGSRVQGSGNIRTLMTGFTFTLENYPTAEV
                     NREYLLVQTTLFIQDNAQHSGQDQHFTYSTSFELHPTGEVYRPQRTLSKPHTKGPQSA
                     IVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDENSSCWVRVSYPWAGKGFGGIQIPRIG
                     QEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGLPGAATQSGFYSHTIGGGPTNANALRF
                     EDKPGGEEVWLHAEKDQRIEVNNNESHWVGNNRLKVIDKTETAIIGEKRSLTVQMDDT
                     SLAGQNKTIQAVQNLRLAAGDSIILSCGKTILQMTSDGIFNITCKNFNITATENGKIN
                     TQSGQLDLNMNDRAADIPPPGTSEKTALQLAIDVTFMNKK"
     gene            complement(6103..6459)
                     /locus_tag="ACHOS5_004662"
     CDS             complement(6103..6459)
                     /locus_tag="ACHOS5_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9779358.1"
                     /translation="MKRFFLLFFLSGCTVGDYVRPLLPGNISLQDGRVCSKPDDGSMI
                     LNYILYEGKYGNVVKLKKLTSQFEPVCIDISELSSDVTYIGKYMLSSGSKKEYYGFSI
                     IRTGERILRLDDNHIK"
     gene            complement(6456..6986)
                     /locus_tag="ACHOS5_004663"
     CDS             complement(6456..6986)
                     /locus_tag="ACHOS5_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFP9779359.1"
                     /translation="MSLRRMTSGELILARSIFENTIDYNKVWIHNEKYLPGQGERTAM
                     TPNGEMYYPDAVYSSDFSSQSMPGERETVAGASHLFIHEMMHVWQYQKGYAVKLRGLF
                     SWAANYHYDLNLQSIASYSMEQQASIVADYWLLINYGLVADLANVNYVGDTTESVNTL
                     SEKYKKVLNLFPAGVI"
BASE COUNT         1535 a         2074 c         1666 g         1831 t
ORIGIN      
        1 cgcttcagtc gcccgccggg gtcgtaaagc tgccgcgtgc tcagtcgccc ctggcttctg
       61 agcacttcac ggtgcaggtg gtcgcgcgtg aactcgctga tgacctcccg gtccaggttt
      121 atctgctgga gatggccgct gccgtagtac agatggcgct gcgttctgcc gtccggaaag
      181 gtggtggcag tccggttgct gagttcatcg tactgatggg tcaggatgcc ctgcgccgtc
      241 tcctcgctca gcatccgccc cagtctgtcg taactgaaac ggataatctc cggggcgtcc
      301 gtttccccgg catccggcct ccggcggcgg atttccagta gctggtctgc cgcatcatgg
      361 acgtattccg tacggctacc ggctgtctcc tttgccgtga gccggcccgc cgcgtcacgc
      421 gccagccggt gacggatttc accgccccgg tccccggtga acaccacttc cgtgaccgcg
      481 ttcagcgcgt tgtagcggta cgcctggcgg cagccgtccg ggtttatctg ctccgccaga
      541 cggtccagca cgtcatggcg gaaccggtag ctttctccgt tctcgtttgt cagcgtgacc
      601 agacgcccgt aagcatcgta cccgaacgcc gtcctgcgcc ccgtcgcgtc tgtccggctg
      661 cgcacccggc cctgcccgtc ccgctgccag cgcgttatcc tccccatcgg gctggtgtac
      721 ttcaccagcc tgcccgccgc gtccgactgg taattcaccg tgcggccgtc cgccagtatc
      781 acctccgtca ggtggccgct gtcgtcatac ccgtagcgcg tgctgtgact ctccgcatcc
      841 gtcattctga tgagccgcgt gcgttcgtcg tagaaccacg ccgtctgcga gccggagcag
      901 tccgtgtgcc gcatgagctg cccgtcttca ttccactgga ggtatttatt ccccccgcgc
      961 gcatccgtta tctgcaccac ctgaccgtgg cggtcatact gataccgggt gttctgctgc
     1021 agcgggtcgg tcacggcaag aaggttgccg cgttcatcgt acacacagcg ccagctgttt
     1081 cccgccgcgt ccacctccgt ttccggctga tgccacaccg ggtgccactg cgcctgctcc
     1141 acccgtccca gcgggtcgtg cgtctccgtg atatgcccct gccggtcata cacatagcgc
     1201 catttgccgc cctgcgcatc cgtcattccc agcagcagcc gttcttcgtc gctccagcgg
     1261 aacgtcgtca cctgtccggc ttcgtcctgg tacgccgtca cctgattctg cgcgtcccac
     1321 tgatgataac gcgtgcgccc cagcccgtcc gtcacgcaca gcctgcgtgc cgccaggctc
     1381 cacgccagcc ggtactgttc accgctgtcc gtgtgatgcc ccgtcacccg ccatgccgcg
     1441 tcatccgggc cttcatacca gccccactgg taataatacc cttcgccgcc cggctggcgg
     1501 tgcagcgtca tgcagtggtt ttcgtcgtaa gcaaactcac gcagcaggtg ccccgtgctg
     1561 tcgtacacgc cgttcaggtc gccggccccg tcatagcggt aacgacgcag cagtaaaggc
     1621 gtcgtatcag ggaaatgctg ataaatctgt gtcacccgcc ggggatgcgc cgccagttcg
     1681 taatacagac ggatgcacgg acgctgctgc tcgccgctga tttcagtgat gcgtccccgg
     1741 tcgtcgtagt acagattcag acagttgctg ttgcggtcgc ccagcatttt cagccgctgg
     1801 cgctgcgggt ggtgcgggtc ggcctcaaac agaaaaaact gtccatcatc gctgctgata
     1861 agccagtgcc cctgctcacc ccgccgtacc gccagtcctt cgccggggct gtaaaacccg
     1921 ccccccggca gtacggcttc catctccagg cgtcgtccca tacccgacac aaaggtcatg
     1981 cagttttcgt ctgcgctacc cggtgtgcgc acgaggctga cttcatacat tacactccag
     2041 ccctgaccaa acatgccttc cgtgcgttcg tcacgactgc tgtacacgcg ctgccagtcc
     2101 agcggaaagt ggcccggcag actgaaatcg aagtcatcat caccggccag gtatttggcc
     2161 ccggtggcgg cactgaccgg atgcggaagg gctgtggcgg ccttctgcgt cagaatgctc
     2221 atcccgatac cggcagcaaa acaggcgatt tcagaaagca ttttacccgg gcgaagaaac
     2281 atcagcgcca cggttgccac cagggttatc tgcgacctgc cgcttctgat atccctgacc
     2341 accagtaacg gaccgccaat gcggacatcc ggtgacacaa acgccccgtt ccccggcgca
     2401 ttcaccaccc tggcctcgca ggtacagcgt gccccgctgc gtgctgccgg ctggctgttg
     2461 atacgcacat ccctcgcgcc ttccgcgata tattccgccg cgggcggttc gtcatcgcct
     2521 ttgaacagat tactgatgct gccaataacc gcattcactc cctgaaaggc ggtttccagc
     2581 ataccgtccg gactcaacag attaaggagc gttcccggag ggtcatccag aaaggcggct
     2641 tttttttgtg cgagtaactg cgtcagagag gagggatgtt tatggcagtc gattttatcg
     2701 tcaggagtca gggtggagcc aggagccgca ccggccaccg tgggctgcca tagttccgac
     2761 acaaaatgtg tcacgccatc caccatttca gaacgccctt cttccgtact gagctggtcg
     2821 tgcagcgccc gggacatcag cacaggccct gtcaccgggt taactgccag ggctatggcc
     2881 cccgccattt ccagatattc cccggccaca tcccggaacg ttctggcctc ctcctgacgc
     2941 tgtgcttctt cctgttccgc cttcgcctgc gcttcctgcg cctcaatgtc ctgccgcgat
     3001 gccgcagtcg ccgctgcccg cgcggcaggt ttgctgttaa tccatgtgtc accagagccg
     3061 gtgaggatgt aaccttcagt ctgaggcggg aaaagcatat ctgcgagggc tgaacaggct
     3121 tcgctgacct tttctgtcac gccggtgtac accatcgcca cacccgccac aaaaccggtc
     3181 agcacgacgg ccgtgcaccc catcgtgatc cccactgacg ccgccagtaa cgaggttgtg
     3241 ccgacaatga gggcatcaat aacgacattt gcggccacct ccagcaccgc ccccagtaca
     3301 tctgccataa acgacgtatg cagcagggca tcaccctgtc ttgccgccca gaatgcctct
     3361 tccatgtccc gttccctgtg tgattaattc gtcccggctt cctgccgcat cctgaagcgc
     3421 agattctttt taatttcagc ccagcgcgtc atatctgcca gggtcagcgg tgtggttcgg
     3481 gcataggtga aggccatcat gacccgctca cccggcaata caaatgccac ctgacactgg
     3541 tacacctgct cgttaccccg gagaaactgg ctctgtattt ccagcccttc ttcaccatca
     3601 ttaatcccca cccgtgttac ctgaacgggc gtgtagtgaa gatcctttac ctgtttctcc
     3661 agttttttca tttgctgttc aatctgggag gttaagtcgt ctccttcctg catctggccc
     3721 cggctgataa tgagtgcagc gtcgggatcg ctgaggcgca gcatattaat cgtactgtcc
     3781 tgaataatat tcccgggaaa agttacaaca gtttcattga gataatactc cgtcataatg
     3841 gtatgaatcc tttttaataa atgaattgtt tatggatgga ttttatttat tttttattca
     3901 tgaaggtgac gtctatcgcc aactgaaggg ctgttttctc cgatgttcca ggtggaggta
     3961 tatctgccgc tcggtcattc atatttaaat caagctgccc tgattgtgta tttatctttc
     4021 cattttctgt tgcagtaata ttaaaattct tacaagttat attaaatatt ccgtcactgg
     4081 tcatttgcag gatagttttc ccacaactaa gaattatgga atctcccgca gcaaggcgta
     4141 aattctgtac ggcctgaatc gttttatttt gtccggcaag ggaggtatca tccatctgaa
     4201 ctgtaagaga acgtttctca ccaattatcg ctgtttctgt tttatcaatg actttaaggc
     4261 gattattgcc cacccagtgg ctttcattat tattaacttc aatccgctga tctttttcgg
     4321 catgtagcca gacctcttca ccgccaggtt tatcctcaaa acgtagtgcg ttggcattgg
     4381 ttgggccgcc gccgatagtg tggctgtaaa acccgctttg cgtcgctgcg cccggtagtc
     4441 cccacggtgg catggtgtcc tggttgtagg tacgcccaac gattatcggt aaatccggat
     4501 caccgttttt gaaatccacc agtacttcct gaccaatgcg cgggatctga atgccaccga
     4561 atcctttacc cgcccacgga taactgacac ggacccagca ggaactgttt tcgtcattct
     4621 taccgtaacg atcccagcca aactgaactt tcacccggcc atatttatct gtccagattt
     4681 cctgccctgc cgggccggtg acgatggcac tttgcgggcc tttagtgtgg ggtttgctca
     4741 gggttcgttg tggacggtaa acttcgccgg tgggatgcag ttcaaagctg gttgagtaag
     4801 taaaatgctg atcctggccg ctgtgctgcg cgttgtcctg tataaatagc gtggtctgta
     4861 ccagcaggta ttcccggttc acttcggctg ttggatagtt ttccagggtg aaggtaaaac
     4921 ctgtcatcag ggtacgaata ttgccgctgc cctgtacccg actaccgggg ctacgctgtt
     4981 cttccattcg gatgcgtgtc agcatttcac cttcactctt tgtgaagtag tctcccggcc
     5041 attcgtagtg ctcatattca gcaaggccgg tttcacgcgg attagccaca gtactcttca
     5101 gcagtgaacg cggcttcata aagtcgaaat cattcagcac ccactgtccg ctgcgcaggc
     5161 tttcatttgc tgtgatggta tggataaact ccttgtccag tttcagccct ttctggtaat
     5221 aacacaccag cggcgaatct gcgcatgcct tgtggacatt aatggcatca gccagcacta
     5281 acgtgtgact gttctcgttg tgctcaaacc accagtagat accccactcc tgcatcaggc
     5341 gctgaataaa atcgaaatct gtttcaccgt actgcacctg ccaggcgcga gtcggatagt
     5401 tttctaccaa tcgtttttcc actggccagg ggtactcatc cagtacctca tccaggatgt
     5461 ccaccacgtt tttattctga aacgctttgt agtcactagt gtgggtcagt gtttttaacc
     5521 acggttcgag tcgcagttca tacaccaccg agcgtccttg atggccgacc accctcgccg
     5581 ccgtcaccag cccgctgata taacgttttc cgccgccatc caattcgata ttgacgcaca
     5641 gatctttgcc caccatcggt ttgagctgca ggttggctgc gggggaaaca tagtccaggt
     5701 tcaggatgtc aggagttttt aatttaatag aataagtaaa tagttcccct actgtttctg
     5761 tcccccctaa gcttgcaaac agtaacattc catcaggaac tgcgctgccg ctcaaagtca
     5821 cattatgtgt acccgatcca gctagtgcgc cggatggata atcagtagct gcaaaactca
     5881 taattaatcc ttttaactag aaatggctaa gtgctaataa gaaatcttac agagaatgag
     5941 atatgcaata tattcgtagc tggcataaaa catctcaata cttatgcatg ctactgaaga
     6001 tactattaac catatatgcc ttgaagaatg caccgtcatg ctaatctggt gaaaatattc
     6061 atctcaagca cattaataaa accaatgaaa ttatttaatt tgttatttga tatgattatc
     6121 atcaagtctt aaaatacgct ctcctgttct aattatgcta aaaccatagt attctttttt
     6181 acttccggaa gagagcatgt attttccgat ataagtaaca tctgaagata gctcacttat
     6241 gtcaatacat acgggttcga actgactagt caattttttc agttttacta cattgccata
     6301 tttaccttca tataaaatat aatttaaaat catactccca tcatctggtt ttgaacaaac
     6361 ccgtccgtct tgtaaagata tattaccggg caacaatggg cgaacataat caccaacagt
     6421 gcatcccgac aaaaagaaca ataaaaaaaa tctcttcata tgacgcccgc aggaaataaa
     6481 ttaagcactt ttttgtattt ttccgatagc gtgttcactg attcagttgt atcacctaca
     6541 tagttaacat ttgccaaatc agctaccagt ccgtagttaa tcaatagcca ataatcagct
     6601 actatagatg cttgttgctc catggaataa gaagcaattg actgtaaatt aagatcgtaa
     6661 tgataatttg ccgcccagga aaataaacca cgcaacttaa ctgcataccc tttctgatat
     6721 tgccaaacat gcatcatttc atgaataaac aagtgagaag cacctgctac tgtttcacgc
     6781 tcaccaggca ttgattgcga tgaaaaatct gaactataaa cagcatcagg ataatacatt
     6841 tccccgtttg gggtcatcgc tgttctttcc ccttgaccgg gcaaatattt ttcattgtgg
     6901 atccatacct tgttataatc aattgtattc tcaaaaatcg atctggctaa tattaattca
     6961 ccagatgtca ttctccttaa tgacatatcc tttctcctgg aaataatatc tttcagataa
     7021 aaataaacgc tctttgatgt ccaatatacc cactttatgc agtattgatg gcatcatctt
     7081 catagaaaat gaaatcaaaa caagtt
//
LOCUS       DAZKHK010000191         7098 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKHK010000191 DAZKHK010000000
VERSION     DAZKHK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.192"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..260)
                     /locus_tag="ACHOS5_004664"
     CDS             complement(<1..260)
                     /locus_tag="ACHOS5_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9779360.1"
                     /translation="MTYEVIITSEAEQQIINLHRYITEKAGSAIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGV"
     gene            complement(266..523)
                     /locus_tag="ACHOS5_004665"
     CDS             complement(266..523)
                     /locus_tag="ACHOS5_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9779361.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            659..937
                     /locus_tag="ACHOS5_004666"
     CDS             659..937
                     /locus_tag="ACHOS5_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779362.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWLQHPPCTEEQAEW
                     LIQCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            939..1988
                     /locus_tag="ACHOS5_004667"
     CDS             939..1988
                     /locus_tag="ACHOS5_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9779363.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAAWMIDVIRHAMNDTQERE
                     LSLAELCWWAVCHQVADALPESVLRRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            2001..2375
                     /locus_tag="ACHOS5_004668"
     CDS             2001..2375
                     /locus_tag="ACHOS5_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9779364.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKSGERYRRAVALIVRQQ
                     QLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            2372..3193
                     /locus_tag="ACHOS5_004669"
     CDS             2372..3193
                     /locus_tag="ACHOS5_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9779365.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            3421..3618
                     /locus_tag="ACHOS5_004670"
     CDS             3421..3618
                     /locus_tag="ACHOS5_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9779366.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            3769..4821
                     /locus_tag="ACHOS5_004671"
     CDS             3769..4821
                     /locus_tag="ACHOS5_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9779367.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELES
                     ERFLQTVKEIRDDFCR"
     gene            4910..5632
                     /locus_tag="ACHOS5_004672"
     CDS             4910..5632
                     /locus_tag="ACHOS5_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779368.1"
                     /translation="MAVVCSVILVCSPINIFIEKDKLSLVPGMVILVTRNIRELFRTY
                     TDRVKVADINHDIVSQYLEGGRRRMSSPQACFPHYLTSGIHQPVLAEALITNHGFPSA
                     PSGDLSTMTGLSCLSLFANDESLSSFLSRCLSNTSDKVRAIIQSDISVNWKLGVIALQ
                     LHMSESLLKKKLKDEGNSFSRLLLEERMRGAVNMLCFQYGRGAIVAEKCGYSSLSYFI
                     SVFHRYYGVPPDKYVSLHMVDF"
     gene            6710..>7098
                     /locus_tag="ACHOS5_004673"
     CDS             6710..>7098
                     /locus_tag="ACHOS5_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9779369.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYND"
BASE COUNT         1773 a         1643 c         1963 g         1719 t
ORIGIN      
        1 acgccagcga taaagacttt attgccatca acggcaaaag caatgatcgt tctgtggcgg
       61 aagtgagtta cccgcattcc ctgtcgaata tcatcgcgtt tattgccccg atgcgggaat
      121 gtggaaaaac catcaagata atcaagaagc gcgttggcat aattgtcagc aatggcgctc
      181 cctgccttct ctgttatata tctgtgcagg ttgattatct gctgttcggc ctcagaagta
      241 atgatgactt catacgtcat gcagattact tcccggctcg aatcgcggcg cgaacctgtg
      301 aaatggagcg tccgttgttt gggttttcgc ggatagaatc gagagatggg gcggctgaat
      361 gcgttaacca cgcttcgatt gctttatcgc gctcattcag tgcgcgaagc ccttcacgaa
      421 tgacctcact ttctgaagca taggcaccgg aagccacacg ggcgcgcacc atgtcagcca
      481 tctcgttagt taatgtaatg ctgaattgtt gggttgtacg catggtaaac ctcacggagt
      541 aggatagaac actattcgat gatagcacac tgcctgttga cgacaccaga aatcagatac
      601 aatattgccg cacgccagcc tgaacaactg gcacctgctg cgccagcaga gacaaccgat
      661 ggcgcacgat accagatttt acaattcgga taactccgcc gcccctgcca gcaggcacgg
      721 gcggcgttct catgcattca aatctgactg gttacagcat cccccatgca ctgaagaaca
      781 ggccgaatgg ctgatccagt gttaccgcag gcgcggatat gagtttgaga aagccctcag
      841 cttcgatcgt cgtcactgga taatctccgt caggctccct tattccgaac gcccaccgcg
      901 tccgtcccgc acattccagc agcgcatctg gaggtaacgt gcgggtatta cttcgacctg
      961 ttctggtacc ggaactcggg atggtgctcc ttaagccggg ccgtgaatca atgtcagcat
     1021 tccataacgg cagaatattg gtggaaccgg aaccgaaaaa catgcgcggt ctgccgtccg
     1081 gagtcgttcc tgccgttcga cagccgctgg cagaggataa aacattactg ccatttttca
     1141 gcaacgaacg ggtgattcgt gctgcgggtg gcgctggtgc actgtctgac tggttattac
     1201 gtcacgtgaa atcctgccag tggccacacg gtgattatca tcacagcgaa accgtcattc
     1261 accgttacgg taccggcgcg atggtgttgt gctggcactg tgacaaccag ctgcgcgacc
     1321 agacatcaga atcactcgat caacttgctc agcagaatct ggcagcctgg atgattgatg
     1381 tcatccgtca cgcaatgaat gacacacagg agcgggaact gtcgctggca gagttatgct
     1441 ggtgggctgt ttgtcatcag gtggctgatg cgcttccgga gtctgtattg cgtcgttcgc
     1501 tgggattacc agcggaaaaa atccgctccg tataccgtga gagtgacatc gtaccgggag
     1561 aacagacagc catcagcata ctgaagcagc gcacaaaaaa tattgcgctg ccacttcacg
     1621 tccaccagca acaaaatcca ccacagaaaa aaacggttgt cagtatcgct gttgatccgg
     1681 agtctcctga atcgttcatg aggcggccta aacgtcgccg ttgggttaat gagaaataca
     1741 cgcgctgggt aaagacacag ccgtgtgcgt gttgtggtaa gcctgctgac gatccgcatc
     1801 acctgattgg tcatggtcag gggggaatgg ggacaaaggc ccacgatatt ttcacgctac
     1861 cgttgtgccg ggagcaccac aacgaacttc atgcagaccc gctgaagttt gagaaaaagt
     1921 acggctctca gattgagtta atttttcgtt ttcttgatca cgcctttgcg actggcgtgc
     1981 tcgggtaaaa gaggtgactg atgctcatag atttggtttt accttacccg ccgacggtga
     2041 acacttactg gcgacgccgt ggcagcacat attttgtatc aaaatccggg gagcgttatc
     2101 gccgggcagt ggcacttatt gttcgccagc agcagttgaa attaagcctg tccggacggc
     2161 tggcaataaa aattattgca gagccaccgg ataagcgtcg tcgtgacctg gacaatattc
     2221 tgaaagcacc gctggatgcg ctgacgcacg cggggttgtt aatggacgat gagcagtttg
     2281 atgaaatcaa tattgtacgt ggccagccag tatctggtgg acgtctgggg gtgaagattt
     2341 accccataat gcttgaaggg caggtcaaaa aatgaaactg gaagatttac cgaaatacta
     2401 ctccccaaaa tcccccggcc tgactgatgc atcggcctca acgtcgaaag atgcgctgag
     2461 tatcactgat gtgatggccg cgcagggcat gacacagaat cgggctgaga tggggttttc
     2521 tgcgttcctt gggaaaatgg gcattagtat gaatgacaga gagcgggcaa cagaattgct
     2581 gacagaatat gcactcagtc ggtgtgatcg cgtggcggcg ttaagaaaac tcccggcaga
     2641 aataaaaccg gcagtgatgc gtattatggc ttcgtatgcg tttgaagatt atgcccgtag
     2701 cgcggcgagc aaaaaacagt gcccctgctg tcacggaaaa aaatttattg aaagcgaggt
     2761 ttttacaaac aagatccagt atccggatgg taagccgcca gtgtgggcaa agtgcacaaa
     2821 aggcgtgtat ccgtcttact gggaggaatg gaaaaaagtc agggaggtgg taaaagttgc
     2881 ctgtccggag tgtggaggga agggagaggt atccactgcc tgtaaagatt gtcgtgggcg
     2941 cggtgttgcc attcatcgtg aagagtcgga aaaacagggt gtgccggttt tcagaaactg
     3001 tcagcgttgt ggtgggcgtg gctatgaaag attaccttca acggaggcat ttaatgccat
     3061 atgtaatgta accgatgcca tatctctcga tacatggaaa aaaacagtta aacgttttta
     3121 cgatacgctg gtggtgcagt ttgatattga agaagcatgg gcagaacaac aactgaaaaa
     3181 ggtgaccaga tagctttgtt gatttttccc gaatctgtgg taaatttgcc ctaacgatgg
     3241 gtgttttatg cctgacgtta gaagattttt tacaccccgc cgcctggcgg gtttttttat
     3301 gactgaaatc acgccagtac agtaaacgcg ctggtggcgg tgaataccgg tctttcagct
     3361 tgctggcttt ttcgacaaga gttattggtg tgtcacgtta accggaaaag ggaaaaagac
     3421 atgctgaaac agcaggatat gaccgaaacc gccagagtgg tgtttaatga attaagcgtc
     3481 accgaaccgg cgactgtcgg ggagattgca cagaatactt acctttcacg tgaacgctgc
     3541 cagttaatac tgacccagct ggttatggcg gggctggcag actatcagtt cggttgttac
     3601 agacgccttc agtcctgaag gcttttttat ttgtggtaaa tgggcggctg gtgggtgtta
     3661 ggggcactca ccagccatct gctcatgcgt ccggatcaca agcaaacctc aggcccactg
     3721 ctttgcgcaa aagcagaatg agcctatcag agacaggctt aatgatccat gcttagtact
     3781 gtaaaaatat ccagttgtga gttaatcaac gccgactgcc tggaatttat ccggtcgtta
     3841 cccgaaaatt ctgttgacct gatagtcacg gacccgccgt actttaaagt gaagcctgag
     3901 ggctgggata accagtggaa gggcgacgat gattacctga agtggctgga ccagtgtctt
     3961 gcgcagttct ggcgggtgct gaaacctgcc ggaagtcttt acctgttctg tggccatcgc
     4021 ctggcatctg acattgaaat catgatgcgt gaacgcttca gtgtgctgaa ccatattatc
     4081 tgggcgaagc cgtccggacg ctggaacggg tgcaacaagg aaagcctgag ggcgtatttc
     4141 cccgccacag agcgcattct gttcgcggaa cattatcagg ggccgtatcg tccgaaagat
     4201 gccgggtatg aggcgaaggg cagggcactg aaacagcatg tgatggcccc gctgattgct
     4261 tactttcgtg atgcacgtgc tgccctgggg ataacggcaa aacagattgc tgatgccaca
     4321 ggaaagaaaa acatggtgtc gcactggttc agtgccagtc agtggcagct accggacgaa
     4381 agcgattatc tgaaattaca ggcgctgttt gcccgggtgg cagaagagaa gcatcagcgc
     4441 ggagaactgg aaaagccaca tcaccaactg gtcagcacat acagtgaact gaaccgacag
     4501 tatacggaac tgcagagtga atataagcat ctgcggcggt atttcggtgt gacggcgcag
     4561 gtgccgtaca ccgatgtgtg gacgcataaa ccggtgcagt actatcccgg gaaacatccg
     4621 tgcgaaaaac cggcagaaat gctgcagcag ataatcagcg cgagcagtcg tccgggtgac
     4681 ttggttgcag atttcttcat ggggtcgggc tcaaccgtca aagcagcgct ggcactgggg
     4741 cgtcgtgcaa ctggtgttga gctggagagt gaacgttttt tacagacagt gaaggagatc
     4801 cgggacgatt tttgcaggtg acagaggttg aggaaaggtt gaagggagga tcatttgtta
     4861 ttgttgtata taattattct acccctttcc tgagtcaggt aaatatataa tggcagttgt
     4921 ctgttctgta attctggttt gttccccgat caatattttt attgagaaag acaaactttc
     4981 acttgtgccg ggcatggtca ttctggtcac cagaaacatc agggagcttt ttcgcactta
     5041 cacagaccgg gttaaggttg ctgatataaa tcacgatata gtcagtcaat atctggaagg
     5101 tgggcgaagg cggatgagct ccccacaggc atgttttccg cattatctca cttctggcat
     5161 tcatcagcct gtactggcag aggcgttaat tactaatcat ggatttccca gtgctccttc
     5221 tggtgatttg tccacgatga cagggctttc atgtctttct ctgtttgcca acgatgagag
     5281 cctgtcatca tttttgtcaa ggtgtttaag caatacaagc gataaagtca gggcaataat
     5341 tcagagcgat atatccgtaa actggaagct tggtgttatt gcgttacaac tgcatatgag
     5401 cgaaagcttg ttaaagaaaa agcttaaaga tgaaggaaac agcttcagcc gcctgttgct
     5461 ggaagagagg atgcgggggg cagtaaatat gttatgcttt caatatggac ggggagcgat
     5521 agtggcagaa aaatgcggtt attcaagcct gtcttacttt atatcagtat tccacaggta
     5581 ttatggagta cctcctgaca aatatgtgtc tctgcatatg gttgactttt aatttcatcc
     5641 tgctggcgga tggagtgctg gtggtcggca ttgtggtggt gctttttcct ttggttaaaa
     5701 aggacatgcc aggtagttag cgggtatcag tcatgcccag aaaattttaa atgtctcaca
     5761 attcagacgg ttgacagttg tctgttttgc ggggagtttg ttaaaagaaa ctggcatggt
     5821 gaatccccct gagcggaggg gcgactggtg catcaggtgt tatctttgat acccaggaaa
     5881 gtgaacgcgc gggttcggtg gcaccgggct gaactcaccg ggaggcaccc ggcactatgc
     5941 gcatgatgta cagatacgcg gcttcagccc ctctccggag gggcttttct gtgccttatg
     6001 catcacagtt tctggaaccc tgagtactac agtatcagtc agggtgctat atttgcagat
     6061 gtgatgaaag cctgccggca ggcagggcgt atcggaaatg acccagtaag agaaaacgtt
     6121 gactcagata ccggtgctga gttaccggga aaccggcatc acatgaccgc tatccttcat
     6181 ggctctctca ggagagcctt tttactggag aaaaccgctt ccccgtaaaa tgccgtgttg
     6241 ctcacaattc agtcagttga aggttgtctg cccgactgag aatttgttaa aaaatttctg
     6301 catgatgaat ccccctgtgc ggaggggcgg ctggtgtacg gaatggtctc tgatgatcgt
     6361 tagcgagaat gacgcgggtt cggtggcacc gggctgaact caccgggagg cacccggcat
     6421 catgtaatga acggtacata gcgctactct tcagcccctc tccggagggg ctttcttatg
     6481 gataaaaaaa gcccgcatgg tgagatgcgg gcagcaagga ataaacaaca aaacgtgaag
     6541 taatcaattt ttcagctggc gaataatacc cgacagtaat cactctgcgc aactgcgcgg
     6601 cctttttcgt attgcgggct gtagtcttcc tcctgccatt gtcctgtaac ttccggactt
     6661 cagcccgccc ctcatctgac tcacaacatt atcccgaccg ggaggattca tggcatttaa
     6721 acactatgac gtggtcaggg cggcgtcgcc gtcagacctt gcggagcgac tgactcaaaa
     6781 actgaaggag gggtggcagc catttggcag tcctgtcgcc atcacgcctt ataccctgat
     6841 gcaggccatt gcggcggaag gtgatgtcac cacacctgtg gtggtgaagc cgtcggatgg
     6901 agaaggcaca gttatcagcg ccaccagcga cccggagtat tactttgttg tggttctggc
     6961 agggcagtca aacggcatgt cgtatggtga aggccttccg ctgccggaga catatgaccg
     7021 tccggacccg cgtattaagc agctggcgcg ccgaagtacg gtgacaccgg gaggtgccgc
     7081 ctgtaagtat aacgacat
//
LOCUS       DAZKHK010000192         7000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKHK010000192 DAZKHK010000000
VERSION     DAZKHK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.193"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            198..1073
                     /gene="rluB"
                     /locus_tag="ACHOS5_004674"
     CDS             198..1073
                     /gene="rluB"
                     /locus_tag="ACHOS5_004674"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP9779370.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1113..1703)
                     /gene="cobO"
                     /locus_tag="ACHOS5_004675"
     CDS             complement(1113..1703)
                     /gene="cobO"
                     /locus_tag="ACHOS5_004675"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9779371.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1700..2458)
                     /locus_tag="ACHOS5_004676"
     CDS             complement(1700..2458)
                     /locus_tag="ACHOS5_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP9779372.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEEIGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2678..3727
                     /gene="sohB"
                     /locus_tag="ACHOS5_004677"
     CDS             2678..3727
                     /gene="sohB"
                     /locus_tag="ACHOS5_004677"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP9779373.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3763..4014)
                     /locus_tag="ACHOS5_004678"
     CDS             complement(3763..4014)
                     /locus_tag="ACHOS5_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP9779374.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4394..6991
                     /gene="topA"
                     /locus_tag="ACHOS5_004679"
     CDS             4394..6991
                     /gene="topA"
                     /locus_tag="ACHOS5_004679"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9779375.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSTA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1782 a         1665 c         1898 g         1655 t
ORIGIN      
        1 tcgaaacaag ctttaaccat aaagatagtg tagtatgttg cgcctcaaag ccaggccagt
       61 taaaacgtcg agtcgtttac ttaaggcctg aagagttcaa acagacttta ctaccagtga
      121 gtcagatgta agttctgact cattgttgca caaaagcata tcagacgcct gggaaggcga
      181 caccaaaagg aagctctatg agcgaaaagc tacagaaagt gctggcgcgt gccggccacg
      241 gttctcgccg tgaaatcgaa tctattattg aagccggtcg tgtgagtgtt gatggcaaaa
      301 ttgctaaact cggcgatcgc gttgaaatta cccctggact gaaaatccgt atcgatggtc
      361 atctgatttc ggtacgtgag tccgctgaac aaatttgtcg cgttctggcc tattacaaac
      421 cggaaggtga gttgtgtacc cgtaacgatc cggaagggcg tccaacggtg tttgaccgtc
      481 tgccaaagct gcgtggcgca cgctggattg ccgtaggtcg tctggacgtt aacacctgtg
      541 gtctgttgtt gttcaccacc gacggtgaac tggcaaaccg tttaatgcat ccaagccgtg
      601 aagttgaacg tgaatatgcc gtgcgtgtat ttggtcaggt tgacgacgcg aaactgcgtg
      661 atttgagtcg tggcgtgcag ctggaagatg gtccggcagc ctttaaaacc atcaagttca
      721 gcggcggcga agggatcaac cagtggtaca atgttactct gaccgaaggc cgtaaccgtg
      781 aagttcgtcg tctttgggaa gcggtaggtg tgcaggtgag ccgcctgatt cgtgttcgtt
      841 acggtgatat cccactgcca aaaggtctgc cacgcggtgg ctggacggag ctggacctcg
      901 cccagactaa ctatctgcgc gaactggtgg agctaccgcc agaaaccagc tctaaagtcg
      961 ctgtagaaaa agaccgtcgt cgcatgaagg cgaatcagat tcgtcgtgcg gtgaaacgtc
     1021 acagtcaggt gagcggcggt cgtcgttctg gcggacgtaa taataacggt taatcaaagt
     1081 atgccggacg tcatatccgg catttttaca gattagtaat caatccctat ctgcgctttt
     1141 acaccggcat caaacgcatg tttgacgggg cgtaattcac ttaccgtatc cgccagttca
     1201 agaatatccc gatgacaacc acgacctgtg ataatcaccg tctgttgatg tggacgttca
     1261 tttaacgcct gcaccacttc ttccagtggc aaatagtcat acgccaccat atacgtcagt
     1321 tcatcgagca aaaccatatc cagtgaggaa tcagccagca tccgctttgc atgttgccag
     1381 acttcgcggc aggcggcggt atcagactct cggttttgtg tatcccaggt aaagcccgtt
     1441 gccattacct gaaactcaac gccgtgtggc tccagcagat ttcgttcgcc attaggccag
     1501 gtgcctttaa taaactgcac gacgcccact ttttttccgt gaccaactgc gcgtgttgcc
     1561 gtaccaaatg ccgcggtggt tttgcctttt ccattgccgg taaagacgat gataataccg
     1621 cgttcatcct gggcctgggc cacacgagca tctacttttt ctttcactcg ctgctgacgc
     1681 tgttggtagc gttcatcact cattgggaaa ttcctggttt acggcccggt tgggcgtcaa
     1741 aggtcatgcc ggttttacgg cggctgtcat cgcccatcag ccagaggtag agcggcatga
     1801 tatcagcggg ggttttaagt ttctgtggat cttcggtcgg gaaggcgctg gcccgcattg
     1861 cggtgcgcgt accgcctggg ttaatgcagt tgacacgcag gcgctgctga tattcatcgg
     1921 ccagtacctg catcatccct tcggtggcaa atttcgacgc tgcatatgcg ccccagtttg
     1981 cacgcccctg acgtccaacg cttgatgagg taaagaccag tgaaccggca tccgatttga
     2041 gtaataaagg aagcagtgcc tgtgtgagca taaaggtggc attaacgttg acctgcatga
     2101 cgtcctgcca gacctgcgga tcttgttcgc tcattgggca aacatcgccg agcaatccgg
     2161 cattatgcaa aacaccatcc agacgcggat aattaacggc aatgcgctgt gccagttgtt
     2221 ggcaatcttc ggacgtgcag gttagcaaat cgagaataaa ccactgtggc tgacgtccaa
     2281 tttcttcgtt tatgtggctg gctacctgac gtaatttttc ttcattacgg cccaaaagaa
     2341 tcactgtcgc accatagcgt gcatacgtca tcgctgcttc acgaccaatt ccatcgctgg
     2401 ctcccgtcac caggataatg cgatcattga gtaaatcttg ttttggctgg taatgcatgg
     2461 ctactcctca acgacgttgt ctgtcgcgct gccagtgtac tttatgactt tggggcttta
     2521 tgcctgaaac agaagggcat ttcaatcagc ctggggcgat aacaccgcaa aatggatact
     2581 ttgtcatact ttcgctgcaa taacatctct gcgagacggc taaacatgcc tgttgtaaac
     2641 tgtgagccaa agcgttgttt aaccaaggtg gggactcgtg gaattgttgt ctgaatatgg
     2701 tttgtttttg gcgaaaatcg ttaccgttgt gctagcgatt gcggcgattg ccgccattat
     2761 tgtcaatgtt gctcaacgta ataaacgcca gcgtggcgag ttacgagtca acaatctcag
     2821 cgaacagtat aaggagatga aagaagaact ggccgcggcg ctgatggaca ctcatcagca
     2881 aaaacagtgg cacaaagcgc agaagaaaaa gcacaagcaa gaagcgaaag ccgcaaaagc
     2941 gaaagctaaa ctgggcgagg tggtaactga cagtaaaccc cgcgtctggg tgctggattt
     3001 taaaggcagc atggacgccc atgaagtgaa ctcgctacgt gaagagataa cggcggtact
     3061 cgcagcattc aaaccgcagg atcaggttgt gctccgtctg gaaagcccag gtggcatggt
     3121 gcatggttac gggttggcgg cttcgcagtt gcagcgtctg cgtgataaaa acattccttt
     3181 aactgttacg gtagacaaag tcgctgccag cggcggttac atgatggcct gtgtggcgga
     3241 caaaattgtt tccgcaccgt ttgctattgt gggttccatt ggggtggtgg cgcaaatgcc
     3301 caactttaac cgcttcctga aaagcaaaga tattgatatc gaactgcaca ccgccgggca
     3361 gtataagcgc acgctgacgt tgctgggtga aaataccgaa gaagggcggg agaaattccg
     3421 cgaagagttg aacgaaacgc atcagttatt taaagatttt gtgaagcgta tgcgtccgtc
     3481 tctggatatt gaacaggtgg caacgggtga acactggtac ggacaacagg cagtagagaa
     3541 aggcctggtt gatgaaatca acaccagtga tgaagttatt cttagcctga tggaaggccg
     3601 ggaagtggtc aatgtacgct atatgcagcg taaacgactc attgaccgat tcaccggcag
     3661 cgcggcagag agcgccgatc gattgttgtt acgctggtgg cagcgtgggc aaaagccgtt
     3721 gatgtaaaag acaaacgcga ggataagacc tcgcgttttg ctttaatcaa ccaggtgata
     3781 tttttctgaa agcacatggg ccaggtgttt gaacatatta aacaccgcgg tgcttttggc
     3841 agttggtaat ccttgttcat ctaaaaagta gtcgccggta aataccagca cgccattacg
     3901 ctgcgtgacg ccggtggctt caatccctgc gagcgtatcc tcatgctcac gaatgatttt
     3961 gttggcctct ttcaacagcg tttcgcggtc gatgggttga gtctctttgt tcattattta
     4021 ctccttaaac aaggacatta gtctacgcca ggcatggctt gcagacaaat ataccacgct
     4081 ggtggcaaga gcgccttact ggcaactttg gattttgcat gctaataaag ttgcgtatcg
     4141 gattttatca ggtacagtgt gacgctttcg tcaatctggc aatagatttg cttgacattc
     4201 gaccaaaatt ccgtcgtgct atagcgcctg taggccaaga cctgttaact cagtcacctg
     4261 aattttcgtg agcagagtca cgacaagggg ttgatatccg cagagagcga gtccatatcg
     4321 gtaactcgtt gccagtggaa ggtttatcaa cgtgcgacgc attcctggaa gaatcaaatt
     4381 aggtaaggtg aatatgggta aagctcttgt catcgttgag tccccggcaa aagccaaaac
     4441 gatcaacaag tatctgggta gtgactacgt ggtgaaatcc agcgtcggtc acatccgcga
     4501 tttgccgacc agtggctcaa ctgccaaaaa gagtgccgac tctacctcca ccaagacggc
     4561 taaaaagcct aaaaaggatg aacgtggcgc tctcgtcaac cgtatggggg ttgacccgtg
     4621 gcacaattgg gaggcgcact atgaagtgtt gcctggtaaa gagaaggtcg tctctgaact
     4681 gaaacaactg gctgaaaaag ccgaccacat ctatctcgca accgaccttg accgcgaagg
     4741 ggaagccatt gcatggcacc tgcgggaagt gattggtggt gatgatgcgc gctatagccg
     4801 agtggtgttt aacgaaatta ctaaaaacgc gatccgccag gcatttaaca aaccgggtga
     4861 gctgaatatt gatcgtgtta atgcccagca ggcgcgtcgc tttatggacc gcgtggtggg
     4921 gtatatggtt tcgccgcttc tatggaaaaa gatcgctcgt ggtctgtctg ccggtcgtgt
     4981 gcagtcggtg gcggtccgcc tggtggtcga gcgtgagcgt gaaattaaag cgttcgtacc
     5041 ggaagagttc tgggaagtcg atgccagcac gaccacgcca tctggtgaag cgttggcgtt
     5101 gcaggtgact catcagaacg acaaaccgtt ccgtccggtc aacaaagaac aaactcaggc
     5161 tgcggtaagt ctgctggaaa aagcgcgcta cagcgtgctg gaacgtgaag acaaaccgac
     5221 aaccagtaaa cctggcgctc cttttattac ctctacgctg caacaagctg ccagcacccg
     5281 tcttggtttt ggcgtgaaaa aaaccatgat gatggcgcag cgtttgtatg aagcaggcta
     5341 tatcacttac atgcgtaccg actccaccaa cctgagtcag gacgcggtaa atatggttcg
     5401 cggttatatc agcgataatt ttggtaagaa atatctgccg gaaagtccga atcagtacgc
     5461 cagcaaagaa aactcacagg aagcgcacga agcgattcgt ccttctgacg tcaatgtgat
     5521 ggcggaatcg ttgaaggata tggaagcaga tgcgcagaaa ctgtaccagt taatctggcg
     5581 tcagttcgta gcctgccaga tgaccccagc gaaatatgac tccacgacgc tgaccgttgg
     5641 tgcgggcgat ttccgcctga aagcacgcgg tcgtattttg cgctttgatg gctggacgaa
     5701 agtgatgcct gcactgcgta aaggcgatga agatcgtatc ttaccggcag tcgataaagg
     5761 cgatgctctg acgctcgttg aactgacacc agcccagcac tttaccaagc ctccagcccg
     5821 tttcagtgaa gcatcgctgg tcaaagagct ggaaaaacgc ggtatcggtc gtccttctac
     5881 ctatgcgtcg atcatttcga ccattcagga tcgtggctat gtgcgagtag aaaatcgtcg
     5941 tttctatgcg gaaaaaatgg gcgaaatcgt caccgatcgc ctggaagaga atttccgcga
     6001 gttaatgaac tacgacttca ccgcgcagat ggaaaacagc ctcgaccagg tggcaaatca
     6061 cgaagcagag tggaaagctg tactggatca cttcttctcg gatttcaccc agcagttaga
     6121 taaagctgaa aaagatccgg aagagggggg tatgcgcccg aaccagatgg ttctgaccag
     6181 cattgactgt ccgacttgtg gtcgcaaaat ggggattcgc acagcgagca ccggggtatt
     6241 ccttggctgt tctggctatg cgctgccgcc gaaagagcgt tgcaaaacca ccattaacct
     6301 ggtgccggaa aacgaagtgc tgaacgtgct ggaaggtgaa gacgctgaaa ccaatgcgtt
     6361 gcgcgcaaaa cgtcgttgcc cgaaatgcgg cacggcgatg gacagctatc tcatcgatcc
     6421 gaaacgtaag ttgcatgtct gtggtaataa cccaacctgc gacggttacg agatcgaaga
     6481 gggtgaattc cgcattaaag gttatgacgg cccgatcgtt gagtgtgaaa aatgtggctc
     6541 tgaaatgcac ctgaaaatgg ggcgtttcgg taaatacatg gcctgcacca acgaagagtg
     6601 taaaaacaca cgtaagattt tacgtaacgg cgaagtggca ccaccgaaag aagatccggt
     6661 gccattaccg gagctgccgt gcgaaaaatc agatgcctat ttcgtgttgc gtgacggtgc
     6721 tgccggtgtg ttcctggcgg ccaatacctt cccgaaatcg cgtgaaacgc gtgcgccgct
     6781 ggtggaggag ctttatcgct tccgcgaccg tctgccggaa aaactgcgtt atctggccga
     6841 tgcgccacag caggatccgg aaggtaataa gaccatggtt cgctttagcc gtaaaaccaa
     6901 acagcaatat gtctcttcgg aaaaagacgg aaaggcgact ggctggtcag cattttatgt
     6961 tgatggcaaa tgggttgaag ggaaaaaata acctttaatt
//
LOCUS       DAZKHK010000193         6906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKHK010000193 DAZKHK010000000
VERSION     DAZKHK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.194"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..1282
                     /locus_tag="ACHOS5_004680"
     CDS             80..1282
                     /locus_tag="ACHOS5_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9779376.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDGSSWASPDTASASETDST
                     AVGAWANAGKAGTALGSASYAHAPFSTSVGMSAYVNPEASNGVAVGSWASADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRMNSQQRQINENHEEMKRAAAQ
                     SAALAGLFQPYSVGKFNATAAVGGYSDKQAVAVGMGYRFNEQAAAKAGTAFSDGDVSW
                     NVGVNWEF"
     gene            1410..1616
                     /locus_tag="ACHOS5_004681"
     CDS             1410..1616
                     /locus_tag="ACHOS5_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779377.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGD
                     LKELSLSELEERALMYAADSFRYE"
     gene            1679..2155
                     /locus_tag="ACHOS5_004682"
     CDS             1679..2155
                     /locus_tag="ACHOS5_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP9779378.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLNGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDRNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYGFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            2251..2727
                     /locus_tag="ACHOS5_004683"
     CDS             2251..2727
                     /locus_tag="ACHOS5_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9779379.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDTN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCSANDISHLYRAFMSVEKLTLSGRQVNV
                     LMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLYKGMLLVRKGRL"
     gene            2802..2990
                     /locus_tag="ACHOS5_004684"
     CDS             2802..2990
                     /locus_tag="ACHOS5_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9779380.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            3134..3979
                     /gene="mscS"
                     /locus_tag="ACHOS5_004685"
     CDS             3134..3979
                     /gene="mscS"
                     /locus_tag="ACHOS5_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9779381.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIGGIAARMISGVLNNLMLGRNI
                     DATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNLA
                     AGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIVN
                     FSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMNF
                     VVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSRS
                     VSSRQ"
     gene            4105..5325
                     /locus_tag="ACHOS5_004686"
     CDS             4105..5325
                     /locus_tag="ACHOS5_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFP9779382.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            5514..>6906
                     /locus_tag="ACHOS5_004687"
     CDS             5514..>6906
                     /locus_tag="ACHOS5_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP9779383.1"
                     /translation="MAKLINISGVTEERMTAGEKRVASRLEAFMSNECLVWYDIPVGR
                     KNRHPDFVIIDPENGLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRY
                     ACDTVNALPADSRLRQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIF
                     PSAQTICQDDMTQSVLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHS
                     QIKIMDIQQEILARNIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNIT
                     LASYLRECIESRGLKSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVN
                     SGTITDTGYDAVLVDEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNF
                     SLASVGIQAQGRTSVLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEG
                     TEPEILRCASESDE"
BASE COUNT         1701 a         1382 c         1943 g         1880 t
ORIGIN      
        1 ggtactcttt cacttctctc ctgattatat attaatttaa ctctgtgcca gtatattgtg
       61 tatatcaatg aggttgttta tgaaaataaa tcgacacggt attactttat cgcttatttc
      121 ctttctgatt gccggtgctc cggcgtttgc gcaggatggc agttcctggg cgtctccgga
      181 cacagcctct gcatcagaga ctgactccac ggcagtgggg gcatgggcga atgccgggaa
      241 ggcagggaca gctcttggct ctgcatcgta tgcgcatgcg ccgttttcca catccgtggg
      301 gatgtctgct tatgtaaatc cggaggcgtc taatggtgtg gcagtaggta gctgggcatc
      361 tgcagatgct ttaaattcag tagcactggg tacgggtgca caggcaatgc acaggtattc
      421 agtggctctt ggtcagcagt ccggtacaac ccgtgaaaat gaagtctaca tcggatatga
      481 ctcaggtgtc acaaacccgg ccagtccgcg tgtaccggat aaaacccggg ttctcggtgg
      541 tgtcagtgat ggtacacggg atacggatgc agccactgtc gggcaactga accggaaggc
      601 ggatgaggtg tacagtgacg tcagtggacg gattgcagcg gaagcactta aggcgcgtga
      661 ccataccgat acggttgctg cagagaacag ggagaatatt atccggaaca cggtggccat
      721 aaaccgtaac accagggggc tgctttccca gagagacgtg ctggagacgc atgaagagcg
      781 cctgaacagc cagcaacagc agataaatac cggaagtacc gttgctgtgg acagtcatgg
      841 ttatgtgacg cgcggtgagg gaaccggtga acgaattact gtacaggaag ggctggtacg
      901 tacacaggaa atggccacag aaaaccgtgc tgcggtaagc cgtaaccgtc aggtgggtga
      961 acgcaacagt cgcgccattg cttctcaggc acgtaccctg caggcgcatg agcgtcgtat
     1021 gaacagtcag cagcgtcaga ttaatgagaa tcatgaggaa atgaagcggg cagcagccca
     1081 gtcagcagcg ctggccggtc ttttccagcc atacagtgtg ggtaaattta acgccacagc
     1141 ggcagtgggc gggtacagtg acaagcaggc tgttgcggtg ggcatgggat atcgctttaa
     1201 cgaacaggct gcggcaaagg caggtactgc gttcagtgac ggtgatgttt cctggaatgt
     1261 gggtgtcaac tgggagttct gaacccgggt ctgagtcacc ggggcgggca gcgttctgta
     1321 tgcggtcatg attcgctgcc tgcttttctc cccggtggca tgactgacgg ttccggggta
     1381 ttcctgagga gctgagaggg agagtgtata tgctgaacta tcgcataagt tcagtgcagg
     1441 tatttactgt aaacggtcgg gctgacaggg tgcaggttcg tctggaatgc gacgatgcag
     1501 ctgtttttga atgttgtctt cttgctgaag gagaagggga cctgaaagag ctgagtctgt
     1561 cagagctgga ggagcgggca ctgatgtatg cggcagacag tttccgttat gaatgatgag
     1621 tcagttatac cggtaatggt aaacggagcc ggtatccggg agataagggt ctgagagtat
     1681 gcagattatt attatgaccc gtgacagata tctggaatat ggcctgatgt gtatgctgaa
     1741 cggatacagg ctcacgacag gccgtgaact gtttgatgcc ggaaagcgcc gtctgccact
     1801 tcccgaagac agttatgtga ttctctgtga ccgtaatctg gaaaggctta catactgtat
     1861 gttctgtggg cgtcgttttc ttgtcattcc tgtttcctct gtgagatgcc tgacagatat
     1921 caggcaggcc atccgtcgtg gggcgtggct gttcggacat acggcaagac cactgacccg
     1981 taccgagatg gtggtggtct tcggagttgt tttccatgag tacggtttta cctttctggc
     2041 agaccagctg gggataagca tgaagacggt atgtgcgcat ctttacaatg cgatggagaa
     2101 aaatggtctg cgcggcgtca gtattaaata tctctgcagc accgcagacc ggtaaatccg
     2161 ggataattaa tgttatgtgg ccaggcgtga taaatttcat gtggaacggg tatgcatttt
     2221 atttgtgata acagttattt tctggaggga atgaaggagt atattaatcc cggtaaattc
     2281 attatcagcg gcatgcagac agataatgtg gtgataatat cttcttcccg gatatctgaa
     2341 attgtcggtt ttctgtataa gaataatata agtgatacga acacccttta tttttcttct
     2401 ccggaagcgc tccgttttct ctggggagtg gttgacagtc cggtttatga tatcagacat
     2461 cttactgagc gatgttcagc aaacgatatc agtcatctgt accgcgcatt catgtcggtg
     2521 gagaagctga ccctgtcagg ccggcaggta aatgtactga tgatgctgct ttatggcagt
     2581 aacggaacag agggggcacg atatcttggc atgtcagaga aaacattctc cactcataag
     2641 cagaatctga taaagaggtt acgggtacat aatgaagttg agttgttgta caaggggatg
     2701 cttctggtca gaaaagggcg gttgtgaagc agggcagctt atttccggta atgttaaacg
     2761 cagcctctga ttcgttaatt tcataatcat aaggagtttt tatgcgtctg ttgattgctt
     2821 ttcttcttcc ctggctgact tttttcacca tcggccggcc tctgacgggg attttttgtc
     2881 tggtgttaca gataacgctg ctgggctgga taccggcaac aatatgggcc gtgtatgcgc
     2941 tttcgcagta taagacggaa cagaaaatcc gcgaagccac ggggaaatga tgactgcaga
     3001 agagttggtt gtcataccgg tgtatatgat gcgggataaa ggcccggaga ttcctggaaa
     3061 taaaatgttc cggggaatat aaaacaggga aaaaccggat gtgatggacg cttctggcga
     3121 ggcggtttca tgaattgtcc gtaatcagga tatgttgccc ggttacgccg cgaatcttgt
     3181 ggcagcagtg gttattatta tcgggggtat cgctgcacgg atgatttctg gtgtgctgaa
     3241 taatctgatg ctgggcagga atattgatgc cacagtggcg ctgttccttt ccgcgctgat
     3301 acgtcatgcc attatagcct ttacgctgat tgctgccctg ggacgtgtgg gggttcagac
     3361 agcttccgta attgcagtcc tgggggctgc aggtcttgct gttggacttg ccctgcaggg
     3421 gtcattgtca aaccttgcag ctggtattct tctcgtgatg ttccgtcctt tcagggctgg
     3481 tgaatatgct gacctgggtg gtgttgccgg gactgtgcag aatgtacata tattctcaac
     3541 cacgatgcgt acactggatg gtaaaattgt tgtcattccg aacgggaaga ttattgccgg
     3601 agagattgtt aatttctcca gggagcctga acgacgaaat gagtttatca tcagtgtttc
     3661 atatgatgca gatattgact gtgtgaaaca agtcctgaca gatattgtga tgtcagaaga
     3721 gagggttctg aagaacaggg aaatcactgt tcgcctgaat gagcctgggg agtcatccat
     3781 gaattttgta gtacgggtat ggagcaggcg tgatgacctt cagtctgtat actgggatgt
     3841 actggagaga attaagcggg agtttgattc agaagggata agttttccat atccacaaat
     3901 ggatatccat cttgttcgct ccgggaagca ggcagagtcg gtaacccata acagtcgctc
     3961 agtcagttcc agacagtaaa atgaaaagcg cattttattt gtgaaagatg aacagcctga
     4021 atctgtcagg ctgttttcat tgtgttttta tggtcatttt tggtatatgt actgtatata
     4081 atgtcatctg tacaaggata taaaatgcca aagaaaataa tgacagggat tgatgttttt
     4141 gacgctgcca tggagcgtat tgaatgggtg tttgatacgt tttcatcagt ttgtctttct
     4201 ttttccggcg gaaaagactc cactgtcctg tttcacctga tggcgaatat tgcccgccga
     4261 aaaaaacgcc gtttttctgt actgtttgtt gactgggagg ctcagtatca gtgtaccatt
     4321 gagcatgtca ccaggttgag ggaaatgtac gatgatatca cagatacttt tttctgggtg
     4381 gcgctgcctc tgacgaccgt taatggagtg tctcaggtac agcctgagtg ggtatgttgg
     4441 gaggacgggg ctgaatgggt tcgccagcca ccagaagatg ccataaccag agcgtctttt
     4501 tttccttttt atcagaatgc gatgacattt gaagagttta ttccggcatt taatcattgg
     4561 tttgcccggg aaaaagggat ggttgctgcg attcttactg gtgttcgcgc tgatgagtca
     4621 ctgaatcgtt ttatgggact ggtatccgga cgtaagctcc gttatgcgga ggataaaccc
     4681 tggaccacgg catcggctga gggattttac tacaccatgt atcctttgta cgactggaaa
     4741 acaagagatg tatggattta ccatgcgaga acacgtgctg cctataactc tctttatgac
     4801 ctgatgtaca gggctggagt tcctcttcgc aatatgcggg tgtgtgagcc ttttggaccg
     4861 gagcaacgta aggggttgtg gctttatcat gtactggagc cggatacatg gggcagaatg
     4921 tgtgctcgcg tatcaggtgc tgtaggggga gcgctctatg caaatgaaag tggtgcttat
     4981 tttgctttga ggaaacagat aacaaaacca gttcaccata catggcagag ttatgcaatg
     5041 tttctgctgg agtcaatgcc tgagaaaacg gcggagcatt acaagaataa gattgctgtt
     5101 tatcttcggt ggtggcagtc tcatggatgg ccggatggca tccctgatga gcaggataat
     5161 gaccagggat gtaaagatat tccctcctgg cgtcgcatct gtaagacgtt actgaaaaat
     5221 gatttctggt gtcggatgct ggcgtttagt ccgaataagc cccgacatta tgagcgctac
     5281 ctggccagaa tgcgtgaaaa aaggaaagag tggggaatat tgtaaattat atttaaacat
     5341 cagaaatatg acggttaatc caggatagta gctaaaaaaa taagattcct tgctgattta
     5401 tccctgtttg atatgtaaaa tctgaggggc ttataagcaa cggatatacg agtgctacaa
     5461 tatatgataa gttatatata ttaaatgttc atcatctgtc agggggctgt tttatggcta
     5521 aactgataaa tatatcaggt gttaccgagg agcggatgac tgccggggaa aagcgggttg
     5581 catcccgtct tgaggcattt atgagtaatg agtgtctggt ctggtacgat attccggtgg
     5641 gacggaaaaa ccgacatcct gattttgtca ttattgaccc ggagaatggt ctggtatttc
     5701 ttgaagtcaa ggactggacc gttcacacgt tgcatcaggt aaatcacgaa caggtcattc
     5761 ttgaaactaa cggtattctt aaatcagaaa taaatccgct gctacaggtt cgtcgttatg
     5821 cctgtgatac cgtaaatgct ttacctgcag actcacgttt gcgacagaat gatggtcagt
     5881 ataaggggcg tctgaatctg gcctgggcgt atggtgttgt gtttacccgt attacccgcc
     5941 agcaactgaa ggctctggct gggaatgatg aaaatgctgt ggaaaggatt ttcccttcag
     6001 cgcagacaat ctgtcaggat gatatgactc agtctgtttt acctgatata ttccgccaaa
     6061 aaattgccgg gatgtttacg atgggattca gaacccgggt gacaccacag gttagagata
     6121 ttctgcgtgc tcatctgttt cctgaaatga ccgtcagaca gcactcacaa atcaaaataa
     6181 tggatattca gcaggagatt ctggccagaa atattggtga tgggcatcgt gttattcatg
     6241 gggtagccgg ctctggaaaa accatgattc ttttgttccg ttgtctttac ctggctgaaa
     6301 ctacccctgg taagattctg gtgctatgtt acaacattac tctggccagc tacctgcggg
     6361 aatgtattga gtccagggga ctgaagtcac gggttacggt attccatttt catagttggt
     6421 gcgcctcaat gataaagcgc catggaattc aggtgactgc tggaggaaag gattacccgg
     6481 agaaatgctt cagtgcgctg gaagatgccg tgaacagcgg aacaattact gatactggat
     6541 acgatgccgt acttgtggat gaggggcatg attttgagag tcgctggctg gctctgatag
     6601 cccgcctgtt tgataacgcg tcccgttcac tgctcctgat gtacgatgac gcacagtcca
     6661 tttacagacg ggaacgggct ctgaactttt cgcttgccag tgtggggatt caggcgcagg
     6721 gacgtacttc cgtcctgccg gtaaattacc gtaatacgaa acgcattctg cactttgctt
     6781 atgcattctc ccgtgagtat tttgagaagc atcagaaccg tgaaataccg tttgttcagc
     6841 cacaggcggg cggtgaagag ggcacagagc cggaaattct gcgttgtgct tctgagagtg
     6901 atgagg
//
LOCUS       DAZKHK010000194         6772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKHK010000194 DAZKHK010000000
VERSION     DAZKHK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.195"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(279..1310)
                     /gene="rsmC"
                     /locus_tag="ACHOS5_004688"
     CDS             complement(279..1310)
                     /gene="rsmC"
                     /locus_tag="ACHOS5_004688"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP9779384.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLATNGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1413..1826
                     /gene="holD"
                     /locus_tag="ACHOS5_004689"
     CDS             1413..1826
                     /gene="holD"
                     /locus_tag="ACHOS5_004689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP9779385.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1795..2241
                     /gene="rimI"
                     /locus_tag="ACHOS5_004690"
     CDS             1795..2241
                     /gene="rimI"
                     /locus_tag="ACHOS5_004690"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9779386.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2256..2933
                     /gene="yjjG"
                     /locus_tag="ACHOS5_004691"
     CDS             2256..2933
                     /gene="yjjG"
                     /locus_tag="ACHOS5_004691"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP9779387.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3319..4554
                     /locus_tag="ACHOS5_004692"
     CDS             3319..4554
                     /locus_tag="ACHOS5_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9779388.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IVDRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEINILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYWAWW
                     ERVESIVTCTDLISNGQFA"
     gene            4818..5846
                     /locus_tag="ACHOS5_004693"
     CDS             4818..5846
                     /locus_tag="ACHOS5_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779389.1"
                     /translation="MTRGNFDFINSNVVFSEADKSLWIDLINTDCIWYDMIINPPDDI
                     RTNKIIKNYLRIMEKYTRESCEKRFIYFLGSRKKVRFSTKKQPRYNPFTKNIVFTLLI
                     GKEERKLRIKYSFCTFTQNGVVKVTPNVRVTEKFLYIEYENGNLCVMSVHDFVNGHYI
                     DLGIHTEVHYVGYTRHPHRRPVDFIHRGLSKMLYSVSNDDNDFFVCFSIFNPRIISLN
                     NKCNVVFNISNSLLDEIDVDNEGRIIENCLIRYFDPKLQDDDKESKRSDLNKMFMDLV
                     ENKKIKKIYVNFQYERNNEYFCFYSKVVPPKFHHFFVLDFSDGKINIDRDQDMMTQMA
                     LCMGYYNY"
     gene            complement(6285..>6772)
                     /locus_tag="ACHOS5_004694"
     CDS             complement(6285..>6772)
                     /locus_tag="ACHOS5_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HFP9779390.1"
                     /translation="NKDDIDINDIAVSLSNICRFAGHLSHFYSVAQHAVLCSQLVPQE
                     FAFEALMHDATEAYCQDIPAPLKRFLPDYKRMEEKIDTVIREKYGLPPVMSTPVKYAD
                     LIMLATERRDLGLDDGSFWPVLEGISATEMFKVIPLSPGHAYGMFMERFNELSELRTC
                     A"
BASE COUNT         1812 a         1494 c         1642 g         1824 t
ORIGIN      
        1 ggggggggac ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta
       61 ccaattccgc caccttcgca taccatcaat tcttaaaaag aattgctacc acggaggcgc
      121 attctagtgg ttttcagctt ttcgtcaata gttaattatc gacagcggtg taattgctgg
      181 aaaaatgtcc atcagcgaat tgtggataat tcgtttgtcg gtcagagaag gcgtgtacgc
      241 cgcatccgac acggtatgcg gcgacatata tcagatactc aacctttctt cgcctggcgg
      301 gtcataatgg cgcgatacac cttgaagcgc ccggtttgcg caatcacttc gtggaaacca
      361 aatgtctcat ccagcacgtc cgggtaaggc aggaaggcgt tcgctacaat tcgcaactcg
      421 ccgccgctgt tcagatgacg caccgcgccg cgaatcagcg tttgcgcggc atccaggctg
      481 gtttgcatcc catcgtggaa cggcgggttg gagatgatca tatcaaaacg acctttcacc
      541 tcggaaaaga cgttactggc aaagacttca ccttcaacac cgttggtcgc aagtgttgcg
      601 cgactggctt ctaccgctgg tgcagaaaca tcacacagag tgaggcggat cttcggtgaa
      661 tggcgcgcaa aagcaacgga aagaacgcct gcgccgcagc cgacatccag cactttacct
      721 ttcgtgtgtg gagttagcgt cgagagcagc aactgactgc cgacatccag accgtcgcgg
      781 ctaaacacgc ccggcagcgt tttgaccgtc aagccatcga cgctgtattc gccccagaat
      841 ttattggcat caaataccgg ctgcttttcc agacgaccaa aatagaggcc gcagcgacgg
      901 gcgctgtcta ccttattcaa cggtgcataa tcggccagca tctgctcggc gctgcgcacg
      961 ccgctgcggt tctcgccaac gacaaaaata tccgtgccca ccggaagcag agaaagtaaa
     1021 ttcatcaact ggaactgggc ttccggtttg ttcttcggcc agtagtagat cagcgtatcg
     1081 caatctgcga cgtcatctgc cgtggcgacc aggctaaaac gggcgttatc ccccatctgg
     1141 cggcttaaca cctgccagtg gtggaattgc tgggtatgag cacggctggc cgcggtatct
     1201 aaacgcgcgg gcaggtcatc ctgtaagtct ccggcaaaca gaatacggct ttgttcgaaa
     1261 tcatcactgt gacgcagcaa gacttcactt gccggggtaa atgcagacat ggaatgctcc
     1321 tcaattgata ctggcggcga ttatagccat atgttggcgc ggtatcgacg aatttgctat
     1381 atttgcgccc ctgacaacag gagcgattcg ctatgacatc ccgacgagac tggcagttac
     1441 agcaactggg cattacccag tggtcgctgc gtcgccctgg cgcgttgcag ggggagattg
     1501 ccattgcgat cccgccacac gtccgtctgg tgatggtggc aaacgatctt cccgccctga
     1561 ccgatcctct ggtgagcgat gttctgcgtg cgttaactgt cagccccgat caagtgctgc
     1621 aactgacgcc agaaaaaatc gcgatgctgc cgcaaggcag tcgctgcaac agctggcggt
     1681 tgggtactga cgaaccgcta tcactggaag gcgctcaggt ggcatcaccg gcgctcaccg
     1741 aattacgggc aaacccaacg gcacgcgccg cgttatggca acaaatttgc acatatgaac
     1801 acgatttctt ccctcgaaac gactgattta ccagcggctt accacattga acaacgcgcc
     1861 cacgcctttc cgtggagtga aaaaaccttt gccagcaacc agggcgagcg ttatctcaac
     1921 tttcagttaa cgcaaaacgg caaaatggcg gcgtttgcga ttacgcaagt ggtgctggat
     1981 gaagctacat tgttcaatat tgcggtcgat cctgactatc agcgtcaggg attgggaagg
     2041 gcgctgctgg aacatctgat cgacgaactg gaaaaacgcg gcgtggcgac gctatggctg
     2101 gaagtccgtg cttcaaacgc tgccgccatt gccctgtacg aaagtttagg ctttaacgag
     2161 gcgacaattc gccgcaatta ctaccccacc acggacggtc gcgaagacgc catcatcatg
     2221 gcgttgccaa tcagtatgta agacaaggtg gaataatgaa gtgggactgg attttctttg
     2281 atgccgatga aacgctgttt acctttgact cattcaccgg cctgcagcgg atgtttcttg
     2341 attacagcgt cacctttacc gctgaagatt ttcaggacta tcaggccgtt aacaagccac
     2401 tgtgggtgga ttatcaaaac ggcgcgatca cttcattaca gcttcagcac gggcggtttg
     2461 agagctgggc cgaacggctg aacgtcgaac cgggtaaact caacgaggcc tttattaatg
     2521 cgatggcgga aatctgtacg ccgttgccgg gggcggtttc cctgcttaac gccattcgtg
     2581 gcaacgccaa aatcggcatc atcaccaacg gctttagcgc cttgcaacag gtacgtctgg
     2641 aacgcacggg cctgcgtgat tacttcgatt tgctggtgat ttccgaagaa gttggcgttg
     2701 ccaaaccgaa taagaaaatt ttcgattatg cgctggaaca agcgggcaat cctgaccgtt
     2761 cacgcgtgct gatggttggt gacactgccg agtccgatat tctcggtggc atcaacgccg
     2821 ggcttgcgac ctgctggctg aatgcgcaca atcgcgagca accagaaggc atcgcgccca
     2881 cctggaccgt ttcttcgttg cacgaactgg agcagctcct gtgtaaacac tgattgcctc
     2941 ccccccgttg atgggtaaaa tagccgcaat ttttcgtttt caacaagcgc ggcgcgatgc
     3001 cgcttactca agaagaaaga attatgacgt tgtctcctta tttgcaagag gtggcgaagc
     3061 gccgcacttt tgccattatt tctcacccca ataattaagc ctaaattaaa gctcttttac
     3121 tttttcaaag tcctttcagt taattgatgt ttggttttat aagtcattta aatacagtta
     3181 gttatgtggc ttctttaatt gaaaattctt tcatgtaact ttaaatcgat caggttgctt
     3241 tcatcaaaaa tctgtacata tgcttgtaca taatgtacaa agcagcagag gtgttttgcg
     3301 atttgtacaa ggtgagtaat ggcgctgtct gatgcgtggt tgcgttcagt cgttggaaag
     3361 gaacgtgata aggttttggt taaatccgat cgtgatggtc tgtctgtcag agtatcaccg
     3421 aaaggtcgcg tagtgttcca atatcgttat caatgggcag ggaaaggtga gcgccttgat
     3481 atcggaactt acccggcaac tggattaaaa gaggccagag aagaagttat ccgtctccgt
     3541 ggtgaactcg agtcaaaccg caatccacga ttagtcaagc aggctgaaaa acgaaaagct
     3601 actgaagcca tgacggtaga gtctgtgatc cgtgcctggt atgaagcata ttgtgtaaaa
     3661 aataaaaaag gttctgaaca gatactccgc tcgtttgagc tgcacctgtt ctctaaaatc
     3721 gggaatatcc ctcacgatgc agctacattg catgattggt tagaagtcct ggagcctctt
     3781 agcaccaaga ctccagcaat agtagaccga ttgctaatta acgcaaagca ggctcatgtc
     3841 tgggcgtata agagaaagct cattgaaact cgcccactgt cggatatcac gggtaaagat
     3901 atggatatcc gtaaaggtca gaaaaaacgg tttctgacac atgatgaaat taacatcctt
     3961 tatgctgcga tcgatggttc tcgaatggtt cctaaatacc gagccttcat taaactattg
     4021 ctgcattttg gttgccgtag ttcagagcta attactgcta gggtggatga ttttgatttc
     4081 ataaataagg tatggactgt accaccagaa cgacataaga ctggggacat aacaggcgaa
     4141 ccgctaaagc ggcccattat tgaaccggtt gaagagctta taaaatacgt tatctctatg
     4201 aacaacggtt ccgatatgct ttttactaag gaaggaagca gggagccagt tggtcggaca
     4261 tcattgcagt cgctgcctta caatttaatg cagttcgcat ggcgacgttt ggggtatcaa
     4321 tttcctcatt ggtctcttca tgatttgaga cgaacagcac gaacaaactt ttctgatctt
     4381 actgctcctc atattgctga aataatgctt ggtcataaac tgccaggtgt atggcaagtt
     4441 tatgataaga gcgattatct agaagaacag cgtaaagctt actgggcatg gtgggagagg
     4501 gttgaatcga tcgttacttg tactgactta atctccaacg gacagtttgc gtagccagag
     4561 cgtaatcata tctgccagtc cgctgcggat accttgtgta ctgggttttt agccagacta
     4621 cgttaaccgg tagcttcagt atttggggtg tcggcaggag tttgatggtt tattgggtta
     4681 ctgtgggact aaggatgtag ggatgccaat cgatgaatta tgaaaaatat catagcacaa
     4741 acagaagcaa gggtttgcga accttctgct acttaattta ttatgtgcag gtgattttta
     4801 atgtgatgaa gtgttttatg actcgcggaa actttgattt tataaactct aacgtggttt
     4861 tctcagaagc cgataagtca ttgtggattg atctcattaa caccgattgt atttggtatg
     4921 atatgataat taatcctcct gatgatataa gaacaaataa gataataaag aactatctca
     4981 gaattatgga aaaatatacg agggaaagct gtgaaaagag atttatttat ttcttaggtt
     5041 caagaaaaaa agtgaggttt tcaaccaaga aacagcctag gtataaccca ttcacaaaaa
     5101 atatagtatt cactctgttg attggcaaag aagagcgaaa gcttcggata aaatattcct
     5161 tttgcacgtt tacgcagaat ggggtagtga aggtaactcc aaatgttaga gtaactgaga
     5221 agtttctcta cattgaatat gaaaatggca atttatgtgt gatgtcagtt catgatttcg
     5281 taaacggaca ttatattgat ttaggaattc atacagaagt gcattacgta ggatatacac
     5341 gtcatccgca tcggcgccct gttgatttta ttcaccgtgg cttatcaaag atgctgtata
     5401 gcgtttcaaa tgacgataat gatttttttg tttgttttag tatatttaat ccaaggatta
     5461 tatctctgaa taataaatgt aatgtggtct ttaatatttc aaattctcta ctggatgaaa
     5521 ttgatgttga taatgagggg agaattattg aaaactgctt aatacgttac tttgatccta
     5581 agcttcaaga tgatgacaag gaaagtaaaa ggtcagattt aaataaaatg tttatggacc
     5641 tggtggaaaa taaaaaaatt aaaaaaatat acgttaattt tcaatatgaa aggaataatg
     5701 agtatttctg cttttactct aaagttgttc cgccgaaatt tcatcatttc tttgtgttgg
     5761 atttttctga tggcaagata aatattgata gagaccagga tatgatgact cagatggcat
     5821 tgtgcatggg gtattataat tattaatatt tcgcctgatg ccaactcaaa atcttttttg
     5881 tcgcatccat tgtggattaa tattcagagg taaggggtat ctgcggaatt tatattttgc
     5941 aggatgagca tacaatacat gatataacat ctgtttttgg cacagagcat atagtcagat
     6001 tatgtttagt tctgtgccat agatggatat tatctcacct cttggctaac tctcattact
     6061 caataactcc agcaaatctg tatattttgc gtgatgccca tttatttggg caggatttaa
     6121 tatcaggatc tggaaaatct ggcctgtatt tctggcctgt cctcctgttt acgctgttcc
     6181 agcgaagcac cgtcgatatt gaaacgccac agaattcggc gacttgtttt gttgtcatta
     6241 agttgctcat tgctacccct ccagcggttg ttcagttgaa agactcatgc gcatgtgcgt
     6301 aactccgata actcgttaaa gcgttccata aacatcccgt aggcatggcc tggcgacagt
     6361 ggaataactt tgaacatctc tgtggcagag atgccttcca gtactggcca gaaagagcca
     6421 tcatcaagcc cgagatcgcg gcgttcggtt gccagcatga tgagatcggc atatttcacg
     6481 ggtgtactca taaccggggg taacccgtat ttctcacgga ttactgtgtc tattttttct
     6541 tccatccgtt tatagtcagg aagaaagcgt ttcagtggtg cggggatgtc ctggcaatac
     6601 gcttctgttg catcatgcat taacgcttca aaagcaaatt cctgcggtac cagctggctg
     6661 caaagcaccg catgttgggc gacgctgtag aagtgtgaaa gatgaccggc aaagcgacag
     6721 atatttgaaa gggaaaccgc gatatcgtta atatcgatgt cgtctttatt ta
//
LOCUS       DAZKHK010000195         6758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKHK010000195 DAZKHK010000000
VERSION     DAZKHK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.196"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..95
                     /locus_tag="ACHOS5_004695"
     CDS             <1..95
                     /locus_tag="ACHOS5_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP9779391.1"
                     /translation="LGRRAIGVELESGRFEQTVREVQNLVSQNG"
     gene            136..211
                     /locus_tag="ACHOS5_004696"
     tRNA            136..211
                     /locus_tag="ACHOS5_004696"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:169..171,aa:Ile,seq:cat)
     gene            219..295
                     /locus_tag="ACHOS5_004697"
     tRNA            219..295
                     /locus_tag="ACHOS5_004697"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:253..255,aa:Arg,seq:tcg)
     gene            309..385
                     /locus_tag="ACHOS5_004698"
     tRNA            309..385
                     /locus_tag="ACHOS5_004698"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Arg,seq:tct)
     gene            555..986
                     /locus_tag="ACHOS5_004699"
     CDS             555..986
                     /locus_tag="ACHOS5_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9779392.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTHE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            983..1147
                     /locus_tag="ACHOS5_004700"
     CDS             983..1147
                     /locus_tag="ACHOS5_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9779393.1"
                     /translation="MTLKLRLAAVALLLFLVVMVDFTSRVMSVLADGVLVVGIVVVLF
                     PLVKKGMPGS"
     gene            1637..3577
                     /locus_tag="ACHOS5_004701"
     CDS             1637..3577
                     /locus_tag="ACHOS5_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9779394.1"
                     /translation="MAFKHYDVVRAVSPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSTNPSVAPEYYYVIALAGQSNGMSYGEGLPLPET
                     YDRPEPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDATGASENSVRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQMVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAI
                     LVHAGRTTVKVDVPSSETGAPTPSPSESEAVSTTTLLSYRASESEGKLTEQGWSAGGG
                     KAEIVADEGATGGTAMKLSKETGKGSWHLEHDAGTGAELLKNGGLISCRFKASGELVA
                     NQYVMALYWPVSSLPQGVTLTGDAGNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLG
                     TLGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKMHVTDITR
                     NATYPVLIDSIAVEVNNADAAA"
     gene            3714..3896
                     /locus_tag="ACHOS5_004702"
     CDS             3714..3896
                     /locus_tag="ACHOS5_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9779395.1"
                     /translation="MTFIHQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            3922..4194
                     /locus_tag="ACHOS5_004703"
     CDS             3922..4194
                     /locus_tag="ACHOS5_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9779396.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            4271..4486
                     /locus_tag="ACHOS5_004704"
     CDS             4271..4486
                     /locus_tag="ACHOS5_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9779397.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTCLTNLYFKIREDRRKAARGE"
     gene            4491..5024
                     /locus_tag="ACHOS5_004705"
     CDS             4491..5024
                     /locus_tag="ACHOS5_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9779398.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5295..5867
                     /locus_tag="ACHOS5_004706"
     CDS             5295..5867
                     /locus_tag="ACHOS5_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9779399.1"
                     /translation="MNMMTVPFYGNSLYVVNHNGEAYVPMKPIVEGMGLDWKTQFRKL
                     QQRFKSCVVEMTIQLPGDTQRRPVACLALRKLAGWLQTISPNKVKPEIRDKVIQYQEE
                     CDDVLYEYWTKGVAVNPRRTSVMVELNQACADMKRDKEIASLFGTGLNEWKSVKAAHV
                     SKIRTLISEANLLINFVLADTGKGKITKTD"
     gene            5881..6015
                     /locus_tag="ACHOS5_004707"
     CDS             5881..6015
                     /locus_tag="ACHOS5_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779400.1"
                     /translation="MISDKLITLVKSLCVLVGISFSLMMVALFLSIAWMALTSAGLVG
                     "
     gene            6238..6444
                     /locus_tag="ACHOS5_004708"
     CDS             6238..6444
                     /locus_tag="ACHOS5_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9779401.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPVQAPCVKPPPPPAWAMMPA
                     SNSLQLLDETFSVSETESSATRQH"
BASE COUNT         1674 a         1556 c         1943 g         1585 t
ORIGIN      
        1 cgctggggcg tcgtgcaatt ggcgttgagc tggagagcgg gcgttttgag cagacggtca
       61 gggaagttca gaatttagtc agtcagaacg gatgatattg cagaattagt tacgtgccgt
      121 tattatcctg cgcccggccc tttagctcag tggtgagagc gagcgactca taatcgccag
      181 gtcgctggtt caaatccagc aagggccacc atcacctacc gccattagct catcgggata
      241 gagcgccagc cttcgaagct ggttgcgcgg ggttcgagtc ctcgatggcg gtccattatc
      301 tgtacactgc gttgttagct cagccggaca gagcaattgc cttctaagca atcggtcact
      361 ggttcgaatc tagtacaacg cgccatattt atttaccagg ctcgcttttg cgggcctttt
      421 ttgtctccgc gccacgcccg gcgcatatca accacagagc ctttcggggg tgagcttacg
      481 gagtggtcag tgtgactttc tctgtgggca gatcgctccc gggcgttggc tcacccacct
      541 aaaggaacgt cacgatgttt ggtattttca aaaagaaaac ccgcagagcg gcagcggaaa
      601 ttaaaaagtt tgagaaacgt gatctggcac aggcggttat taatgctgcc tatctggtgg
      661 cctatgcaga tggtgaatgt gaggcttcag agaaagcgaa gatcgaacag gtattacgta
      721 accagcctgc gttgtccgcg tttacgtcag aaattaatgc catcagtgcc acgatcacag
      781 gtcagcttga caccaacttt aaaattggtc gtcgagcggc gttgcgtgaa attgaagatg
      841 taaaacacga tacgcatgaa gcggaagatg tgctggatgt ggcggtggcc attgctgaag
      901 cggatggtga aattgagccg gaagagcgta aggttctgga agagatcgcc ggtgttcttg
      961 gcctgcgact ggagaaccac ctgtgacatt aaaactgcgt ctggctgctg tggcactcct
     1021 gctgtttctg gtggtgatgg tggatttcac cagcagggtc atgtcggtgc tggcggatgg
     1081 agtgctggtg gttggtattg tggtggtgct tttccctttg gtgaaaaagg gcatgccagg
     1141 tagttagccg ggtatcagtc atgccccgaa aattttaaat gtctcacaat tcagacagtt
     1201 gacagttgtc tgatttgcgg ggagtttgtt aaaagaaact ggcatggtga atccccctgt
     1261 gcggaggggc gactggtgaa tggtatgatc tctttgatga tcgtaagcga gaatacgcgg
     1321 gtttggtggc accaggccga actcaccggg aggcacccgg caccatgcag tatacagaga
     1381 ttaggcatat actagggccc ctcatagcag gggcctttta catgcaaaaa aagccgcccc
     1441 cgggaagagc ggttggcaag aagaaaagca atatgaacaa ttaattaacg ctgcgaataa
     1501 taccttacag taatcaccct gcgcaactgt gaggagtgta tttctttttt gcgggtggtt
     1561 gtctttttcc cttgtgtttc gggacttccg ctcactcctt atcttattca gtacattatc
     1621 ccggccggga ggattcatgg catttaaaca ctatgacgtg gtcagggcgg tatcgccgtc
     1681 cgatcttgcg gaacgaataa ctcaaaaact gaaggaaggg tggcagcctt atggtagtgc
     1741 gctgatttcg acagctggtt atggtgcgga attcatccag ccagttgtga gcgagggaag
     1801 catctcatca cctgaggagc caggcaaccg tccgacgacc tcaacgaatc cttctgttgc
     1861 gccagaatat tactatgtga ttgcgcttgc cggtcagtcc aatggtatgt catacggtga
     1921 aggtcttccg ctaccggaga catatgaccg tccggagccg cgtattaagc agctggcgcg
     1981 tcgcagtacg gtgacaccgg gcggtgtcgc ctgtaaatat aacgacatca ttccggcgga
     2041 ccattgtctg catgatgtgc aggacatgag ccgtcttaac catccgaaag cggacctgtc
     2101 aaaggggcag tacggaaccg tggggcaggg gctgcatatc gccaaaaaac tgctgccgtt
     2161 tataccggcg aatgcgggta ttctgctggt tccgtgctgt cgtggtggtt cagcgttcac
     2221 caccggagct gatggcacat acagtgacgc gaccggtgcc tcggagaatt cagtgcgctg
     2281 gggggtggac aagcccctgt ataaggacct tataggtcga acaaaagcag cactgaagaa
     2341 gaacccgaaa aatgtgctgt ttgccgtggt gtggatgcag ggggaatttg attttggcgg
     2401 tacgccggca aatcacgcag cacagtttgg tgcgctggtt gataaattcc gtgcagacct
     2461 ggcggatatg gctggccagt gcgtcggtgg ctctgctggc ggtgttccct ggatatgtgg
     2521 ggacacgacg tatttctgga agcagaagaa cgaatccacc taccagatgg tgtacggcag
     2581 ctataaaaac aaaacggaaa agaatatcca tttcgtaccg ttcatgaccg atgagaacgg
     2641 ggtgaatgtg ccgacgaaca aaccggaaga agacccggac attccgggta tcggttatta
     2701 cggttcgaaa tggcgtgaca gctcagccac ctggacgtca caggacaggg cgagccattt
     2761 cagctcctgg gcacgccgtg ggattatttc cgaccgtctg gcaacggcga ttctggttca
     2821 tgccgggaga accacagtaa aagtcgatgt tccatcttct gaaactggcg caccgacgcc
     2881 gtcaccgtca gaaagtgaag cggtaagcac cacaacactg ctgtcttacc gggccagtga
     2941 gtctgaaggg aagctgaccg aacagggctg gagtgctggc ggaggtaaag cggaaattgt
     3001 tgctgatgag ggtgcaacag gtggaaccgc gatgaagctg agcaaggaga caggtaaagg
     3061 gtcatggcat ctggagcatg atgccggtac tggcgctgaa ctgctgaaaa atggaggttt
     3121 aatcagttgc cgctttaagg catccggtga actggtggca aaccagtatg tcatggcgct
     3181 ttactggccg gtttcctctc tgccacaggg tgtcaccctg acaggggatg cagggaataa
     3241 tctgctggca tcgttttaca tccagacaga cgcaaaagac ctgaatgtga tgtaccacaa
     3301 tgcgaaagtg gcgacaaata accagaaact gggaaccctt ggcgcatttg ataatgaatg
     3361 gcatacgctg gctttccgct ttgccgggaa taacagcctt caggtaacgc cggttattga
     3421 tggtcaggat ggtacaccgt tcacgctgac gcagtcaccg gtcagtgcct ttgcggcgga
     3481 taagatgcat gtgacagaca ttaccaggaa tgcgacttac ccggtgctga ttgacagcat
     3541 tgcggtggaa gtgaacaacg cggatgccgc ggcatgataa aaaaagccgc cagttgcctg
     3601 agacctctgg cggtgtgaga ttcatggaga atcaaggaaa gatactacca ctttcgtcat
     3661 cggcattttt aaatgaaaac tgtttactta gttaaccata acggtaagaa attatgacat
     3721 ttattcatca gctgatgctg tacttctgta cggtggtctg tgtgctgtat ctcctttcgg
     3781 gtgggtacag ggccatgcgt gacttctggc gcaggcagat tgataaaagg gccgctgaga
     3841 aaatcagcgc cagtcagtca gccggaagca aacccgaaga gcccattact ccttaacaac
     3901 ccctttcagc gagaaaatcc catgtcagaa attacatccc tggtcactgc tgaggcagtg
     3961 aaggacgtcc tgcgctctga agaagtcctg agcgcactga aacagaaact tcgccataac
     4021 ctggaagcgc gtcttgatgc agaagttgat gccattctgg atgagctgct tggtgtacag
     4081 gcagagccac cgactgaagc gggagatacc accgcagaga gcggtgaagt tcagcctgaa
     4141 tcaccggtcg ccgatgcgac tgaacctcaa cccgaatcgg tcatgatgct gtaacgggga
     4201 gtcagggcca tcagtaaaca gctgctggcc tttttcatgt tgtgagcttc cggattgcgg
     4261 gagacggggt atgtaccaga tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc
     4321 ggtgggaacg ggctactggt tcctgcagtt gctggacagg gtttccccgt ctcagtgggc
     4381 ggcaataggc gtgctgggga gtctgctgtt tgggctgctg acatgtctga ctaacctgta
     4441 tttcaaaatc agagaggacc ggcgtaaggc ggcacgggga gagtaagctg atgagcagga
     4501 aactccgcta tggtttatcg gctgccgttc tggcgctgat tgccgcaggt gcttctgcgc
     4561 ctgaaatcct ggatcagttt ctggatgaaa aggaaggtaa ccacaccacg gcataccgtg
     4621 atggtgcggg gatctggacc atctgccgtg gagccacccg ggtggatggt aagcctgtga
     4681 ttcctggcat gaagctgtcg aaagaaaaat gcgaccgggt taacgctatc gaacgggata
     4741 aggcgctggc atgggtggag aaaaacatca aagtgccgct gaccgaaccc cagaaagcgg
     4801 ggatcgcgtc attctgtccg tataacattg gccccggtaa gtgtttcccg tcgacgtttt
     4861 ataaacgaat taatgcaggt gatcgaaaag gtgcctgcga agcgattcgc tggtggatta
     4921 aggacggtgg cagagactgc cgtatccgct caaataattg ctacggtcag gtatcccggc
     4981 gagatcagga aagtgcgctg gcgtgctggg gaattgacag ataagcagaa tattttgctg
     5041 aaaaatgacg ttttctcacg cgggcggata acacgaaatc ctgcgaactg gcaaaatgta
     5101 agtgaataaa gtcaacaaga ttgtttgacg cagaggcacc gtaatggtgc ctttgtcatt
     5161 tctgcgcatc tcacgcgcat ctcacaacac agaacctttc aggatgaccc ttgaggatac
     5221 cggtttggct gtcggtgcct ttctgtgggc tggattcctg tgagacaagg ttcatcacta
     5281 aaaggaaata accgatgaat atgatgaccg tgccgtttta cggcaactct ctttatgtag
     5341 ttaaccataa tggcgaggca tatgttccga tgaaacccat cgttgagggt atggggcttg
     5401 actggaaaac gcagtttcgt aagttgcaac aacgttttaa gagctgcgtg gtcgaaatga
     5461 ccattcagct accaggggac acccagcgtc gtcctgttgc ctgtcttgct ctgcggaaac
     5521 ttgctggctg gcttcagaca atcagtccca acaaagtaaa gccggaaatc cgcgacaagg
     5581 tgattcagta tcaggaagag tgcgatgatg ttctctacga atactggacg aagggcgttg
     5641 ctgttaatcc gcgtcgtacc agtgtaatgg tggagctgaa tcaggcctgc gctgatatga
     5701 aaagggacaa agagattgcc agcttgtttg gtaccggact gaatgagtgg aaatctgtaa
     5761 aagctgccca tgtatcaaaa atccgcacgt tgataagtga ggcgaatctg ctgattaatt
     5821 ttgttctggc cgataccggc aaagggaaaa tcacaaagac ggattgatgg agtggtactc
     5881 atgatatcag acaaactcat aacgctggtg aagagcctct gtgtacttgt cggcatttca
     5941 ttttcgctaa tgatggttgc tctttttctt tccatagcct ggatggcgtt gacttcggca
     6001 gggctggtgg ggtgagcata aaccgaatgc tttccgcgtt taccgttatt ctgctggtgg
     6061 tctgtggtgc gctgtgtctg gcaacaaacc attaccgcga taacgccatc acttacaaag
     6121 cgcagcgcga taaaaaagtc agtgagctga agctggcgaa cgccaccatt actgatatgc
     6181 agcagcgcca gcacgatgtt gctgcgctcg atgcaaaata ctcgagggaa ttagccgatg
     6241 cgagagctga aaatgaaact ctgcgtgctg atgttaccgc tggtcgtaag cgcctgcgga
     6301 tcaacgccac ctgtccaggc tccgtgcgtg aagccaccgc caccgcccgc gtgggcaatg
     6361 atgccagcgt cgaactctct ccagttgctg gacgaaacgt tctcggtatc agagacggaa
     6421 tcatcagcga ccaggcagca ttgagaacgc ttcaggaata tatccgtact cagtgcctga
     6481 aatagttttt tcaagcgttg tatcgtcgcc gtatttccgc attaacagag accgcagcca
     6541 ccttatctgc gtgagtgtgc ggggataatc aaaaacgatg cacaccgggt tttctcattt
     6601 ttcacgagat gggagcgatt tcccgcgaag ctgcctgtcc ggtgcggtgg tggaagaaac
     6661 cggataaaac aaccgcattg tgcaaatatt gatcaaatat ggtgctgctg tgtgaaatct
     6721 gaaaaatcac agcggtcatt atgcatcagt ttttaaca
//
LOCUS       DAZKHK010000196         6735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKHK010000196 DAZKHK010000000
VERSION     DAZKHK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.197"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..251
                     /locus_tag="ACHOS5_004709"
     CDS             <1..251
                     /locus_tag="ACHOS5_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9779402.1"
                     /translation="LNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLI
                     TFLPVFLLPVLGDIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(498..752)
                     /locus_tag="ACHOS5_004710"
     CDS             complement(498..752)
                     /locus_tag="ACHOS5_004710"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9779403.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVTLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPLTNSSGIGVELLPVEMTLG"
     gene            complement(1174..2187)
                     /locus_tag="ACHOS5_004711"
     CDS             complement(1174..2187)
                     /locus_tag="ACHOS5_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP9779404.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(2199..3515)
                     /gene="fucP"
                     /locus_tag="ACHOS5_004712"
     CDS             complement(2199..3515)
                     /gene="fucP"
                     /locus_tag="ACHOS5_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9779405.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(3543..4463)
                     /gene="rbsK"
                     /locus_tag="ACHOS5_004713"
     CDS             complement(3543..4463)
                     /gene="rbsK"
                     /locus_tag="ACHOS5_004713"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9779406.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            4767..5549
                     /locus_tag="ACHOS5_004714"
     CDS             4767..5549
                     /locus_tag="ACHOS5_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFP9779407.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISDRPLPADYVTHCRNASVKVITPDSEDE"
     gene            complement(5551..5777)
                     /locus_tag="ACHOS5_004715"
                     /pseudo
     CDS             complement(5551..5777)
                     /locus_tag="ACHOS5_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         1939 a         1548 c         1433 g         1815 t
ORIGIN      
        1 tgctgaacat gatcgtgatt attcctggtg tggtgccgca cttcttcgta ggtgcagctg
       61 caggggtatt cggtaatgca acgggtggac gtcgtggtgc gattttgggt gccttcgctc
      121 aaggcttgct tattaccttc ctgccagtat tcttattgcc ggtgctgggt gatattggtt
      181 ttgctaacac caccttcagt gatgctgact ttggcgcact gggtattttg ttagggatta
      241 tcgttcgcta atattttgat gacattaata tcctccagga tttcaggaac gtggaggata
      301 ttaattcctc ttgttttctc catttttctt tcttccagtc aggactgcat ccgattaatt
      361 gtaagcgctc cgagaagtac gtagcgtaag gattatttta cagctgagaa gtttcagtgc
      421 agcagttcat gcacacggtt cgacggctag tcattgatct tcataattac ctcgcgtaaa
      481 aaacggggta aggccgctca tcccagcgtc atctccacag gtaataattc aacgccaatg
      541 ccagaagagt tcgttaacgg aagacgccgt gatatacgcc ctttgatcag cgagagcctg
      601 agccatttga acagaagatt atcattgata tcatgctccc gggcaatacg ggcaacagaa
      661 ccccttggtt gtaaagccag tgtaaccatt tgaagtttaa actcatttga aaatgacctg
      721 cggggttcag ctgataatgt tttttgttcc ataacaggtg tccactagtt gaaaaggtgg
      781 gtacctatgt taccaatact ggcttaatgg ctacatacag cagtcggttt acgcatacac
      841 ttttatagtg cactccggcc tcatgaatat aacggtggat taccgccgaa cgaatcggaa
      901 aatagatact ggaaaaaaac tgaaattgtg accagttttt gttgaccact acaaataaat
      961 aaaagagttg cagtgcttgc tcggtaagaa aacaatggaa gttgagatcc tcaaagaagc
     1021 tgtggagtac accactctac agtagttgcg caaatttgga gtggacgata atgtgatcgt
     1081 ctataagggc aacgctatca tagtcttgtc ctggcgggta aaaaagcgcg cttaccttaa
     1141 cgataagcgc gccgctgttc aggccttgag tggttattca attcctgtgg tgactgtaaa
     1201 agtgcgcgtt tgctgcggtg caacctgaat cagcgtgcca ttacgttgcg cggcaagata
     1261 cccctcaggc cgacaggttg caggtaatgc aaaggcggct acctgttgct ctccgttata
     1321 aaggatccag cgtgtcacat aatttagttc agcactgtag aaacgagtaa caaacgtagt
     1381 gccatcggga gagatcatgc gaaactctgg ctgatctgta taagcgtcca gtttgtctgc
     1441 aaagaagaca atttctggat cataaaattc cggttgactc agcgtcgaca gagaggcttc
     1501 tccctgcata atccgttgat taaacgccag ccactgagcg gtgggattaa catgcgaagg
     1561 cactgattca cgcaatctta atatttcgtc cgggatattc tggctgaatg tagcatttgg
     1621 tatatatgca taattcatgt ggcacatata ttgtagtggc atatctacag aagccagatt
     1681 ggttacggcc atcttaatat cgaacagtgt agaggatttg tgaaggacca ctgttggttg
     1741 agccagataa tgatgaccga aacccattac atactcgtaa cgcccgttaa ggcgtaacat
     1801 atctccgtct aattccagcc atgcttcatc catcgcggca caggccattt caccgtgtag
     1861 cagatgagta tcttccgcag atgggcagcc attagccagc aaacctgaat gaaaagcaaa
     1921 acagccatag gtctctatca cctctgtcgc cggtttaggc tggcgaaaca tattgcacat
     1981 ggtgaggccg tgtccatcaa attgcgcatc ccaaatcatc tgccccatcc agggaagaat
     2041 aatcaaatgt ccacgactgt ttgcaatttt aagcccctcg acaccgctgt catagcgaaa
     2101 agacgtgaca gtaaaatcac tattttccag caagatacga ggtttctcgc caaaaagcgc
     2161 ccgccacaaa ttaatacgcg tactcataac ggttctcctc aggacgctgt gacttcagcc
     2221 agtgcggtac gtactttgct ttcacgccag aagtagactc cgacatagac aaagcagagc
     2281 atagaaacca ggaatgaaag ctgtagtgag tggaacatat ctgcaatata tccctgaatt
     2341 gctggaacca ccgcggcacc gacaatagcc ataacaatga ctgctcctgc catttctgta
     2401 tgttcgttat caactgtatc cagtgttcct gcatagatcg tcgcccagca agggccaaac
     2461 aaaacactta ccaggacggc gacatagacc gcgctgaaac ttggagccag tgcaacatat
     2521 gccaggaaca gcgcccctat aacggaatag agaatcaata ctttttccgg attaaaacgc
     2581 gtcataagga tgttggctat aaacttgcca ataaagaagc aggcaaagct atagaccatg
     2641 aagtttgaag catcacgttc gttgatatcg cccaactcca gcgccagacg aatggtaaat
     2701 gaccatactg cgacctgcat acccacataa aggaactgcg ccacaatacc gcgacgaaag
     2761 cgcggatttc tagccagata gcgcagcgta tccattgctg acgggcgttt atggtgactt
     2821 gtctgtgcca ctttacaggt tgggaagcgg gttaaaagga acaacaccat gaccacaacc
     2881 agaatcataa tcatatactt atacggttca agggtgttct ctaacatcag caccttaaag
     2941 ttgtgaattt gctcggcgtt cattcctgac atctgctttt caaggctttc cccctcggag
     3001 aaaaccagat atttgcccaa taaaatacca gacgcagcac caatcggata aaaggtctgg
     3061 ctgatattga gccgcaatgt ggcataggct tttggaccga tcattgaact gtatgtgttc
     3121 gctgcagttt caaggaaact caggccaatc gcaatcgcaa aaatagctgc aaggaacata
     3181 gtgtaggttg ccatatgtga ggcagggaaa aaaagtgtac aaccaccaat atacagcgtc
     3241 aggccaatta aaattgccac cttataactg gtctttttaa tcacaaggga tgctggtatt
     3301 gcaattaaaa aataaccgcc ataaaatgcg ctctgcacca atgctgaagc aaagttactt
     3361 agcgaaaata cacttttgaa ttgagtgatt aatatgtcat ttaatgcagc tgcgcatccc
     3421 catagcggga ataaacacga taacaaaata aactggaaca agggagtctt attcagatac
     3481 ccatcaggca tctgaatgat gtttttatcg ttcatagtgc tacctttaac tgtgcaggat
     3541 gattattcgt ttaaggttaa aaattcatta aattgttcaa tactcggata agatgattgc
     3601 gtacctttcc ctgtgacgct gaaagcggca aagagagcgg cttttttcaa agcggcttca
     3661 acatcaccgc tttgaacata ataatgggaa aagcaaccaa taaatgcgtc accagcgcca
     3721 ctagtatcaa cagcatttac tttgaatgca ggaacatgga cttcctgatc acgggtcatc
     3781 cataatgcgc ctttttcgct catggtaaca ataatattgt tcagcccttt atcaactaac
     3841 gaacgtgcgg ccaaacgaat atgatcataa gtatcaaccg acataccggt taatatttcc
     3901 agttctgttt cattcgggat aaagaaatca catttgcagg cataagacat atctaactca
     3961 cgcaatgccg gagccggatt taataacact tcaataccat ttttcttacc aaactcaatc
     4021 gcgtggtaaa ctgtttccag ttgaacttcc agttgtaaaa cgatcaattt gcattttttc
     4081 agatcttctg cagctcgatc gatatcttcc ggggaaagaa atttattcgc tcccttaatt
     4141 attaatatac tattgctcga gttggcatta acaaagatcg gtgcaacacc actgctggta
     4201 caggggacct tctcaacata agtggtatta attccccatg attcgagatt acgaatagta
     4261 ttatccgcaa aaatatcatc acctacttta gtcagcatca ggacttttga attcaattta
     4321 gccgccgcca ccgcttgatt agcacctttc ccaccacatc cgattttgaa ggcaggtgct
     4381 tccagagttt ctccttcttt aggcatctga ttcgtgtaag taatgagatc caccatattg
     4441 gaaccaataa ctgcaatgtc catttcacta cctcttataa actttcgcat aacaatggta
     4501 tttaaataac attagcatgt tacttttgca tcatttgtga ctgagatcgc gattagcaca
     4561 tcaacccgat gtttatttaa tagacttcca gtcttatcac tcaggccaac actatctaat
     4621 cataagcaac ctaaccggat taataccgaa aattcagcag tctataccct tttcatttca
     4681 aagggtcggt cgtatagtat ggtaactaaa acaatgttta ctaatgccat aatgttattt
     4741 ttataacatt tttatggaga gagttgatgg aaacgaagca aaaagagcgt atccgacgtt
     4801 tgattgaaat acttaagaaa accgacagaa tccatttgaa agacgcggca cgaatgctgg
     4861 aagtttctgt aatgactatt cgtcgcgatc tccatcagga agatgaacct ctgccactga
     4921 ccctactggg tggctatatt gtaatggtgc ataaacccgc accatccatg ccagtaatcc
     4981 aggacgttcc gagaaatcat cgtgatgact tacctattgc aattctggcc gccggaatgg
     5041 ttaatgaaaa tgatctgatc ttctttgata atggccagga gataccgctc gttataagca
     5101 tgatcccgga tgcaatcacc ttcactggca tctgttactc acatcgtgtc tttgttgcgt
     5161 tgaatgaaaa acctaatgtg acagcaatac tttgtggtgg tacgtatcgt gccagaagtg
     5221 atgcttttta cgatgccagt aactcttcgc cattagactc tctcaatccg cgaaaaatat
     5281 ttatttccgc cagcggtgta catgatcact ttggcgtcag ctggtttaat cccgaagatc
     5341 ttgccactaa gcgtaaagcg atggcccgtg gactaaggaa aattttgctc gcccgccacg
     5401 ccttgttcga tgaagtagcc tctgccagcc tcgcaccgct ctctgcattt gatgttctga
     5461 ttagcgatcg tccgttaccg gcagattatg ttacgcactg ccggaatgct tctgtaaagg
     5521 tcattacacc tgattcagaa gacgaatgac ttactgaaaa aacaccgtac tcttgttaaa
     5581 catcgtcgga ttggactgat tacgttgcac tttcatcaca tattccagct tatcaatttg
     5641 gcttatcatc tactccagac gctggtcatc attgaccaat agtctgatat ggcttttgtc
     5701 gctgtcctga atcggcaggc agaaaatgcc ttcaacgtta aaagcgcgac gggaaaaagc
     5761 ccacaagggt gagtcattac gcccggataa taaacaggct cctccctgtt atggaagccg
     5821 ttgtattgtc cataatgtct gggtttttaa tgcacactgg ataacaaccg gacgaatctt
     5881 caaatatcac tggcgctgac agccggtttg aattcatctc acaaccctgc atagagcgaa
     5941 tctcctgcct gcacgtcact ccactccatg gtatcaactt cacactcttt atctgcggct
     6001 agtttcagtc accagataag catcgactat gaaaggtgag ccatgacaac ataacgttgg
     6061 taacgctctg acgccttaac ggaagatgcc tgccaccaca gggaatgtaa acgactgaag
     6121 tgtggccttt aatgccgtga acggctcatg gtctcctggc acggttgccg ccccaacctg
     6181 taacaacatt ccacagtaca atgtctgtca gagccggagc ctcccatgcc tgttgtagta
     6241 actctaccag ttacgaacat ccttcctcaa aagtgttgtc gtatctcgca tggaagaaaa
     6301 gatcctgact aagcagcaac acacaacgta ttgcggaacc tgcatgtttt tcctgtaact
     6361 agtgcattac gacatatggt aatagctacc tgtgtggttt cgctggataa caaggggatt
     6421 tattcgcaag taaaatgcct gataaaatac acgaatctag taatcatcaa tatttactct
     6481 ggtcgaatga cgcgtgaagt ggacggccaa cagacgcagt cagtgatcta cagcctgttg
     6541 aataaaacgc tggatgtctc ccggctctga aattaatgct tcggttattt gcacgaaatg
     6601 ttccttttca aaccctgttg aattttgtta agccggtgca gacacagtaa agcgcgcatc
     6661 aacacctgaa aggtacgata caaacacaag ttacaaacaa taataacgga aggacaatta
     6721 atcagtgagg aataa
//
LOCUS       DAZKHK010000197         6670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKHK010000197 DAZKHK010000000
VERSION     DAZKHK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.198"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..954
                     /locus_tag="ACHOS5_004716"
     CDS             <1..954
                     /locus_tag="ACHOS5_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9779408.1"
                     /translation="LENRLNKIMNGAHTLTFNAVTPCHLAVLPLLASGTLPNYKFNKR
                     PYFILEKTAHATLQINRGLLQQFGETIRVNFQNKNDIEEAFKYASINNMVPISISDSV
                     GSMGGVAPVEFIVRMANRFDGYAYIDDAHGTSIYGINGSGYVMSCLDNKLDDRIIIAG
                     SLSKAFGSHGGFIACNHPDTINFIKTYGTTYAFGGPPSLPGIAACIAAADLHLDGTVS
                     LRQNDLQQIIKYFDNKFNETEVINKRTTMPIRGLLIGNEEKAITICKKMHDRGFATTV
                     AMYPTVEEGKAILRCAISAIHTRENIDSLHKNFMECINEPG"
     gene            complement(1026..3245)
                     /gene="mobQ"
                     /locus_tag="ACHOS5_004717"
     CDS             complement(1026..3245)
                     /gene="mobQ"
                     /locus_tag="ACHOS5_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFP9779409.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPENAPSWCADRSVLWNAVEKAEQRRDSQLAREIELAIPREISRDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKSACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRKVQETQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKA
                     RPVASILSELPLMTWAEPEYRKRQLRFWKLGKQIESLSRTYRAVKERDIPDRRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEVLVG
                     FVRQEDGLRPVLALPSPDYSHLIVSTITENGVCLAGYGEAINHDADTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQCRPGHFRGMSM"
     gene            3424..3702
                     /locus_tag="ACHOS5_004718"
     CDS             3424..3702
                     /locus_tag="ACHOS5_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFP9779410.1"
                     /translation="MSRTLEQKIAEAEVRLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPRPPVKPQKND"
     gene            4230..4925
                     /locus_tag="ACHOS5_004719"
     CDS             4230..4925
                     /locus_tag="ACHOS5_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9779411.1"
                     /translation="MTDLIAYKSNALVEASYKLTLQEQRLLLLCISRLNSSNATPESQ
                     KTMMITAEDFFNSFPDMGRNHAEEKLQEAIDRLWDRSIILKDDEKREEMRWLQYRAHY
                     LKGEAKVQITFADAVMPYLTQLKGQFTKVVIKNISRLSRSYSIRIYEILQQFRSTGER
                     VIALDDFRSMLQIDKKYKDFKILNRSLIKPSIAELNQKSDLAVTVEPIKKGRTVVALR
                     FLFKEDKQIKAPI"
     gene            5296..6411
                     /locus_tag="ACHOS5_004720"
     CDS             5296..6411
                     /locus_tag="ACHOS5_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9779412.1"
                     /translation="MTKIYIYLVLAASLSALSTDMIVPALPELQKAFNADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDHFGKVRVMTICLIIFLIGSIVCFMANNLNMLILGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSATIPLIAPLAGALILDYYNWNVIF
                     IVMGSFSILTMLLSPKETNKQEKKTHQDDIKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMNDYGLNTFNYSVVFSLSVLFFIIGNQLSKIEKISDPWVLFPLNAISVIPFLIL
                     NDNFVICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGVISFL
                     SVKISDNGVSLGTTLSISCLILALLSIFVTIIGNNKK"
     gene            6408..>6670
                     /locus_tag="ACHOS5_004721"
     CDS             6408..>6670
                     /locus_tag="ACHOS5_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFP9779413.1"
                     /translation="MNWQKKKIERSIKYQTRVFSEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKYAITNAIERFGVQLSVARTRVKVDL"
BASE COUNT         1836 a         1478 c         1505 g         1851 t
ORIGIN      
        1 ttggaaaatc gattaaataa aataatgaat ggggcacaca cattaacatt taatgcggtt
       61 actccatgtc atttagctgt actaccgctt cttgcatcag gaactttacc taattataag
      121 tttaataaaa gaccgtattt tattttagag aaaacagcgc atgcaacatt acaaataaat
      181 agggggctgt tacaacaatt cggtgaaaca atcagggtta actttcaaaa taaaaatgat
      241 atagaagaag catttaaata tgcttcaata aataatatgg ttccaatatc tatatctgat
      301 agtgtcggtt cgatgggtgg ggtggctcct gtcgaattta ttgttagaat ggctaacaga
      361 tttgatgggt atgcctatat tgatgatgct catggcacat caatatatgg gataaatgga
      421 tctggatatg taatgtcttg cctggataac aagcttgatg atagaattat catcgcgggt
      481 tcattatcta aagcctttgg ttctcatggt ggatttattg catgcaatca tcctgacacc
      541 ataaacttta taaaaacgta tggaacgacg tatgcttttg gtggcccgcc atcattacca
      601 gggattgctg catgtattgc agctgctgat ttacacctcg atggtacagt atctttgcgc
      661 caaaatgact tacaacaaat aataaaatac tttgataaca aatttaatga aaccgaagtc
      721 ataaataaaa ggactacaat gccaatacgt ggattgttaa ttggcaatga agaaaaagct
      781 atcacaatat gcaaaaaaat gcatgatcgt ggttttgcta caaccgttgc tatgtatcct
      841 acagtggaag agggaaaggc gattctacgt tgtgcaatat ctgctattca taccagagaa
      901 aatatagata gcctacataa aaactttatg gaatgtatta acgaaccggg ttagtaatat
      961 catggcctct ttatatagag gccctctatt tcatttttta tggcccgtgg cgcagccctc
     1021 gaatatcaca tactcatgcc ccggaagtgc ccggggcggc attgttgttg cctgtgctgt
     1081 tcgtcccgct tccactcatg ccaggtctga ttaaaaccga gttgacggaa gagataatcc
     1141 ggcatctctt ccggtttgtg gacagcagcc aaaagcctgt catgatggcc tttgagccgg
     1201 aactgcatca ggtgcggttc cggtgggtac ggagtatccg catcatggtt tatagcttct
     1261 ccgtaacctg ccaggcatac cccattctcc gtgatggtgc tgactatcag atgactgtaa
     1321 tccggcgacg gaagcgcaag aacgggacgc agcccgtcct cctggcgcac aaatccgaca
     1381 agcacttcct ccggcccttt gcacaacagc agattcagcg tcctgctgtt tccctctctc
     1441 gaaaatacaa caggcatgag ttgttccggt acagcatcag cggtgatatc cacccggtgc
     1501 gctgacggca gcggagaggc tacgggcctg cggtcgtcgc tgaaataaaa attatccggc
     1561 cagtgatgcg gcagcgccgg aaaaccgtaa gagggccacc cctggcgcat ctgttccagt
     1621 acatgccgga ccgtcgcacc ggaatacatt ctcacacgct caggccgcgc catcgtctca
     1681 tgagattctg gcattccctc acgtatatcc gagaccatca cagccccgcc cggcgacagg
     1741 taaacattca ccgggtcatg aacactctgg gggtaatgcg ccagctcatc accccatacc
     1801 gtgaattctc cggctctgtt actcaacatc atgtagcggg ttatcctgcc cgttcccggc
     1861 tctctggcac gccccagctc cgtaataacc ccgactaatg ccgtttcacg gctcagacgg
     1921 tggttatcat gacgtttcag ccgcgcctca tgctcctgtt ccttccggcg cgcttccgct
     1981 tcggcttcct ccctgcgccg ctctgcttcc cgtgctgaca gtttttcttt cagctctgcc
     2041 attcccggcg caatccacgt attccactgc gtttcaaagg cctgacgacg gtcgggaata
     2101 tcccgttctt tcacggccct gtaggtgcga ctgagagatt caatctgttt cccgagtttc
     2161 cagaaacgga gttgtctttt gcggtattcc ggttcagccc atgtcatcag gggaagctct
     2221 gacaggatgg acgctaccgg acgggctttt ctgagtccgg tcatctcttt ttcgagaata
     2281 tcccgctctt tcaccagcgc attcccctca cgtttgatgt cgtttgcctt cccgttcatc
     2341 gccacgaact caggggtcat ctcccatgcg ttcccgcatt cctgcggcag tgtggcaatg
     2401 aattcccgca gggtatccgc tgaggcattc gcggcgggaa tggtcagatt aagggcttcc
     2461 gctgcgcgac gtgcggcatc gctgagttcc cgcttacgct gcgtttcctg aaccttcctg
     2521 agcgccagct gcgtgcggga aacctgtatt tccaggttaa ggctgttaat caggcggttc
     2581 tgctcaccgc gttctgtttc catgccccgt ttttccatcg cacaggcaga ctttccgagg
     2641 tgaaggcccg gcgctttctc cagtccctga cgctcgtatg aacgatggtc tatctcttcc
     2701 tggtagccgg cgttagcaag cgctctgttc gcatggtcag cccatgatgc gcgccacgtc
     2761 tcggcgagtg cccggtcgtt ccagtccctg acctttcctg cgaatcccgt ttccccgaca
     2821 gctcttgtgg tcagcatgat gtgcgcatgg ggattagtcc ggtccatgtg atggaacgcc
     2881 acatcggcaa tcatgccctg gctgacaaag ttttccctga caaaagcgag aacggtttcc
     2941 cgtgcggcat cgcgggaaat ctctcgggga atggcaagtt caatctccct tgccagctgg
     3001 gagtcccggc gctgttcggc tttctcgacc gcattccaga gaacggaacg gtcagcacac
     3061 catgacggtg cattctcagg ggtgagaatg accgcatcat caacgccgcg tttacgggtg
     3121 taatcgtggg ttaatccggt acggtcatcg ggtatgacag agccggaacg gtaggcagca
     3181 gaagcaacgg cactgtagcc gttttttcgc gaaatgattt tcatgctgag atgataaatc
     3241 gccatttctg ccgtaatgcc tttttgccgt tttctgtcag ccccgcaggg cgcacacaat
     3301 cgaaacggag tggagattgt ataagtgcgc acttcgtgat gtgccgtgca ttatgccagt
     3361 ttagttttta aactaccagt gacgcgctaa aatgcgattt ttcaccgcgc acggagtcac
     3421 gaaatgagca gaacactcga acagaagatt gccgaagctg aggtcagatt acagcgcctg
     3481 aaggcgaaga gccggagcct ggacacggcg caaaaagtca ttgtgggggc tgccatgctg
     3541 gcgagagtac ggaggccgga agaagcgcaa ctgcgtgcgt tcctgctcca gtttctgcga
     3601 aaggaagtca caagacaggc ggatgtgaac cggatacaac cgctgattaa cgagctggag
     3661 aagctaccca gaccacctgt gaaaccgcaa aagaacgatt gatcgccttc cgctcgccgc
     3721 agccccagcg accgagcgtt agcgagtaag tgggcgagga agcggcagaa gtgccgttgc
     3781 tgaatgtggc acgtacgcac tgttttcaga aaggtgatcc tctctgaacc tgcatgaata
     3841 ctggatttcc acaggtttgc atgttgtttt tgcagaacgc ggcaaactga ttttttataa
     3901 catgttgttt ttttagtgtt ttttgttttc ccgctttgag ttttcaacag gcacgccgtt
     3961 aagtttttag atcttgtttt tatatacttg tttttatact tgttttaggg ttgagatttc
     4021 ttttttaaat caatatgttg caatgacata ggtgaacatt ttgggggtat aggtgaacat
     4081 tttgggggtg gttaggtgaa cattttgggg gtataggtga acattttggg ggtggttatc
     4141 cacagaaaaa ggtgaatcaa aaaaaaaata ttgcacttat ggtaaaggtt aattactttt
     4201 ttttgttaac ctatcaattg gggaacgtaa tgacggattt gattgcttac aaatcgaacg
     4261 cgttggttga agctagctac aaactaacct tgcaggagca acgcttgctt ttgctttgta
     4321 ttagccgatt gaactccagc aatgcgacgc cggaatcgca gaaaactatg atgattacgg
     4381 cagaggattt ttttaattca tttcctgaca tgggtcggaa tcacgccgaa gaaaaattac
     4441 aagaagcgat agatcgttta tgggataggt cgattattct caaagatgac gagaagcgag
     4501 aagaaatgcg ttggctccag tatcgagcac actacctcaa gggcgaagct aaagttcaga
     4561 taaccttcgc agatgctgta atgccctatc tgacacaatt aaaaggtcaa ttcacgaaag
     4621 tagtaattaa aaatatatca agattatcaa ggagctacag tattcggatc tacgaaattc
     4681 tccagcaatt ccgtagcaca ggagagaggg ttattgctct tgatgatttt cgcagcatgc
     4741 tacaaattga taaaaagtat aaggatttca aaatattaaa tagatctttg ataaaaccat
     4801 cgatagcgga attgaatcaa aaaagcgatc tggctgtgac ggtagagcca atcaagaagg
     4861 ggcgaacggt ggtcgctctg cgttttctgt tcaaggagga caaacagatc aaagctccta
     4921 tctaaatgca cttaaaacgc tctcagacgc gttctgacgc aaaaaacgca aaaagacaag
     4981 gaaacaccag gccaaaccga aaaaacgcgt cagaatcgtt tttaggtaga tgcataacta
     5041 tgcacgaaag ctatgcgcga tcaatttcga ccgctccgaa gggcgaacag gtcaaaatga
     5101 caagttttct ccctccagat ggaaggttca gcacggggat tttttacctc cttccatcaa
     5161 cctgaatatg catctggaag tccccattag tttctgctca aacactatcg cagtgctatc
     5221 tcaaaatata aaatataccg atggtgtaag tattttaaat atgataaata agaatatttt
     5281 tatcagggat taattatgac aaagatatat atttaccttg ttcttgctgc atcattatcg
     5341 gcattatcaa ctgacatgat tgtgcctgcg ttaccagaac tacagaaagc atttaatgct
     5401 gactattcat taattcaact aagtattagt ggatttctta ttgtatttgc atttagtcaa
     5461 ctgatatcag gtactatagg tgatcatttt ggaaaggtac gcgtgatgac tatatgctta
     5521 attatatttc ttattggcag cattgtatgc tttatggcca ataatttaaa tatgcttatt
     5581 ttgggtagaa ttctgcaggc aataggtgca ggtgctggac ctgtaatatc aaaagcaatt
     5641 gctaaagagt cttttccacc actaaagcta aaaagagcac tttctgacat atcatcgaca
     5701 agtgcgacaa taccattaat agcacctctg gctggagcat taatacttga ttattataac
     5761 tggaatgtaa tatttattgt aatggggtca tttagcattc taaccatgct cctgtcacct
     5821 aaagagacta ataaacagga gaaaaaaaca catcaagacg atataaaatt tgtgactaaa
     5881 gatttcatct gcgggacaat actcgtttca ctaatgctaa gctcactatt ttgctacata
     5941 tcaatatcac ccgcactatt tatgaatgac tatggattaa atacatttaa ctactctgtt
     6001 gttttttccc tatcagtttt atttttcatc attggaaacc aattatcaaa aatcgaaaaa
     6061 atatcagatc catgggtttt atttcctcta aatgcaatat cagtcatacc ctttctcata
     6121 ttaaatgata attttgtaat atgtgttatt ggagcaatga ttttcaattt aactcttggc
     6181 gcatactatc caatagctaa ttttatctca ttacagataa acggttcacg aactggcttg
     6241 gccgcatcaa ttacaggatt tactcaaaca gtaagcgcag gggtaatcag tttcctttca
     6301 gtaaaaataa gcgataatgg agtctctttg ggaaccactt taagtatcag ctgtcttatt
     6361 cttgcattat tgtcaatatt cgtaactatt atagggaata ataaaaaatg aattggcaaa
     6421 agaaaaagat agaacgttca atcaaatacc aaacaagagt atttagcgaa catataaatg
     6481 aactagtaat caccagaaga aatggtaagg taatcactgt taatgatggg agggaaatga
     6541 ttgagtttgt gtcatgctct tatcttggac tggaaacaca tgaaaattta aaatatgcaa
     6601 taactaatgc aattgaaaga tttggagtcc aactttcggt cgccagaacg cgcgtaaaag
     6661 ttgatttatt
//
LOCUS       DAZKHK010000198         6347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKHK010000198 DAZKHK010000000
VERSION     DAZKHK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.199"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2753
                     /locus_tag="ACHOS5_004722"
     CDS             <1..2753
                     /locus_tag="ACHOS5_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9779414.1"
                     /translation="NGLAGDALHKIGKGTLVVAGSGENPGTLNTGDGTVILAQKADAA
                     GRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDIAFNRLAAADEG
                     AVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMDGGADISGSF
                     TQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESRTFSIGQLK
                     LKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGTSAVGGASG
                     SSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTSSALTVSDGG
                     RLHVKGGLETDGGVTLDRGTLLVDGGSVRNDVYERLLAWSEERGGLNGSGEYDFMTGA
                     AGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFHTLTAGSMD
                     ISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLVTAPADTGA
                     DVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVATRQDAETPNPVEA
                     VSPLPSPVSAPSSAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVNSSAVRDAG
                     MLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNYTHVQVG
                     ADKKHELDGLDLFTGVTMTYTYSNAGSDTFSGKTKSVGAGLYASALFDSGAHIDLIGK
                     YVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTEDAWIEPQAELVYGAVSGKQ
                     FSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETV
                     LRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDNAVNASFRY
                     SF"
     gene            complement(3320..5992)
                     /gene="rcsD"
                     /locus_tag="ACHOS5_004723"
     CDS             complement(3320..5992)
                     /gene="rcsD"
                     /locus_tag="ACHOS5_004723"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP9779415.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1533 a         1465 c         1765 g         1584 t
ORIGIN      
        1 tgaacggact tgcgggagac gcacttcata aaattggtaa gggaacactg gtggtggccg
       61 gcagtggtga aaatcctggc accctgaata cgggggacgg aacggtcatt ctggcacaga
      121 aggctgatgc tgcaggcagg gtccgggcat tcagtgaagt cagtattgtc agcggacgac
      181 cagttgtggt gctgcaggac agtcatcaga ttgaagggga ccgcattcgc tggggatacc
      241 ggggaggtac gcttgatatc aacggaaatg acatagcctt caaccgcctg gctgcggctg
      301 atgagggggc agtactcaca agccgggcgc gtcctgccac agtccgtctg gatttcagcc
      361 agtcagggca gaaggccgtg atgtggcatg gtcattttac cggtaacctc agcgtcctga
      421 acaacacgtc atctgcggtg gattttatca tggatggcgg agcggatata tccggcagtt
      481 ttacacagca gggcggggga ctgtatatcc aggggcatcc ggttgttcat gctgtcagca
      541 gtgaggcggt tgcgactgca ttgcgaaagc agggtgataa ttctgttctg acgcagccgg
      601 tatcatttgc tcagaaggac tgggagagca gaacgttcag catcggtcaa ctgaaactga
      661 agggggcggc attcagcctt tcccgcaatg cgacactgac gggagatatt gatgctgata
      721 atgccaccat ggtgctggga agtgactcgc tatatctgga tatgaaggac ggcacgggga
      781 gcagcagtgc gccggttaag gggacgtctg ccgtgggcgg tgcatccggt agcagtacat
      841 ttcgcgggaa tgtgaatatg cgtcattctg ccctgacggt ccgcgaccat tttactggca
      901 gtataacggc atcggacagc cggatagctg tgagttcaga gaatgtgcgt ctggaaggga
      961 attcccgcct gacctcttct gcacttactg tcagtgacgg cgggcgttta catgtgaagg
     1021 ggggactgga gacagatggt ggtgtgacac tggacagagg aacccttttg gtggacgggg
     1081 gaagtgtcag gaatgatgta tatgaacgtc tgctggcctg gtcagaggag cgtggcgggc
     1141 tgaacggaag cggtgagtat gattttatga cgggggcagc tggtctcctg cggagttatg
     1201 tcagagggag tgcaggtaat gtgaatctgc agaatgcggc ctggatgatg acgggaaact
     1261 catccgtgaa gcatcttgag tcatcgggaa gtgctctgta tttcagcagg ccgggcgggg
     1321 agttccatac cctgacggca ggcagtatgg atatcagcga cagtgttctg gtgatgcgga
     1381 cggaccttaa ccactcagac cagcttcggg tgacagaatc gctgcggggt aagaataacc
     1441 tgttgttggt tgattttact gagcgttctg acgggcagaa ggcgctgaat atccctctgg
     1501 tgacggcgcc ggccgatacc ggtgcggatg tgttcagtgt gaaaacgcgt gataccggct
     1561 tcagtcatat cacacctgtt gtcagggctg aacaggggac gggcggcacg gcctggcagc
     1621 tgaatgttgt gcagccggaa actgccgcag agcctgtggc gacgcggcag gatgcagaga
     1681 cgccgaatcc tgtggaagct gtatcaccgc tcccttcacc ggtttctgct ccgtcatcgg
     1741 ctccggaggc gtcagtgacg gatgtactga ccggagagaa tgcgggggaa tcaggtgagt
     1801 accgtgatga gacccggtgg ctgctgacag gataccggag tacggttaat agctccgcgg
     1861 tcagggatgc ggggatgctg atgtcaatgg ggcaccgtaa cttcattaat gaggtcaaca
     1921 acctgaacaa acgtatgggt gacctgcggg acatcaacgg cgaggccggt gcatgggccc
     1981 gtatcatgag cggaaccggt tctgccggcg gtggcttcag tgacaactac acgcacgttc
     2041 aggtgggtgc ggataaaaaa catgaactgg acggtctgga cctcttcacc ggggtgacca
     2101 tgacatatac ctacagcaac gccggcagcg acaccttcag tggtaaaacg aagtcagtgg
     2161 gtgcaggtct gtacgcttca gccctgtttg attccggggc ccatatcgac ctgattggta
     2221 agtatgttca ccatgataat gagtatacgg cgtcctttgc cggactcggc acaaaagact
     2281 acagctctca ttcctggtat gccggtgctg aagtgggtta ccgctatcat gtcacagagg
     2341 atgcctggat tgagccacag gcggaactgg tctatggtgc tgtatccggt aaacagttca
     2401 gctggaagga ccaggggatg agcctgtcca tgaaggacaa ggactacaat ccgctgattg
     2461 gccgtaccgg tgtggatgtg ggtaaatcgt tctccggtaa ggactggaaa gtgacggcac
     2521 gtgctggtct tggctaccag tttgacctgc tggcgaacgg agaaacggta ctgcgtgatg
     2581 catccggaga gaagcgtatt aagggcggga aagacggccg tatgctgatg tctgtgggtc
     2641 tgaacgcaga ggtccgtgac aacatccgtt ttggtctgga gtttgagaaa tcagcctttg
     2701 gtaagtacaa cgttgataat gctgtcaacg ccagcttccg ttattcattc tgacggttgt
     2761 tactgaaggg gattatggcc tgacggctcc ctgtaaataa agacaggcac agaagattgt
     2821 gagttactta gtgttccacc aacaagtaag gtcgtaactc tgtgcctgct tcgtaagcgt
     2881 acagtgaggg ccatcgtcat aactgaagca tccgacgacg tggtgtctac cagagtgtca
     2941 gatatgcagg aaaatatgag ctccgacaga aatgcagtaa aaaaagagag tctcgtacac
     3001 ccctccagaa aactccaggg ctgtaagttg tattaaaaag ctgatttttc tttagcgata
     3061 cagacctcaa taagtataac acccttcagt tactgtggtt atgcacaggt gttattttta
     3121 actgtgtgga taaacagtgt cgattattca gattggttat ggggcatgta tgggacattt
     3181 ttaatcggcg aaatacgtcg aagttcgtcg atatggtaaa cgaatcatct atccaaccct
     3241 tgaaaaacgg cgctcctgga cgatcttcgt cgatttttaa aatgcaacaa gtacgttgtt
     3301 catgtaatag gctaccttgc tacagcaagc tcttgacata actgtcaatg tcgctgatgt
     3361 atttttctat tcctggaaca tccttctcac gaatcagatg ttccagcgtt tcacataact
     3421 gcttgccggg taccagattt agcatggcaa atacgccttt aagacgatga gccgtttggg
     3481 ctaacgcagc aaagtcactg gttgctgctt cagtatatag cctcttaaca tcatccggta
     3541 ctgtgtctac aaagagcgca taatagccgc tggcatggag ttgcgcattt tcatctccgc
     3601 ccagaggcga ttctgtcacc tcttcctgcg ccagttgcac ttcaattaat tgtaagaccg
     3661 cttcctgcat agcgttgctc atattgaaat tgacgcacaa ttgaccaggc ccaatttccc
     3721 gtacgccaga ctcatcatcg cttaaaagca agccagaggc agtaagatta gacggattat
     3781 ccgttaaaaa gatatcataa tcttgactaa ttaatctttc atcgggtgtg atacaggttg
     3841 caccccaatt ttctaactgg cgagtgacaa tattccgaat ttctgccgaa gtaacatcca
     3901 ccattacgca gacatcatcc agtaatcgct cttcttcctc ttcaacttcc gggtcagctg
     3961 cgagcatttt gatatgcaca gagtagcgtg taccaagccc atcccgcgtt ttgatgttta
     4021 aatgaccgcc cagtttacgc gccagttgat cgctcagcca gaatgccagc gggtccgcct
     4081 tgccatagcg atcgttttgg gtctggttga taaacgggaa gtgcaaatta tccatttcat
     4141 gaatgcttac gccttctcca gtgtccagaa tacggaacgt caggcggtct tcggaggact
     4201 catcctgatc aacctcaagg gtgatttttc ccaattgcgt tgaggtcacg gcatattgca
     4261 tcagtaacag caaaatacgt cgtaaggcat cgcgatcgcc gcggcgcatg tcgtgtgctt
     4321 tcagatgatt gttaatcagc agttgcagac ctttacgctt gatggcaggc aacactgaag
     4381 gcacaacttc atcaattaaa tcctgcacgg agaacagctc cgtctcactt ttccagctat
     4441 cgtccgcaag catgttcgct aactgtattt catcgaccag ccgcaccagc acatctgcct
     4501 gattcgccag ttgtttgcct tccggggcgt tgagtttagc cgcgctctcc gccagggact
     4561 gtgcgggttc tttcagcgca tcgccaatgt ttttcataaa ggtcatccgc ccctgctggt
     4621 ttttctcata cagacgctgc gcctgcttga gtttcttgtt taccagcact tcgcgatcct
     4681 gatcgcgaat aatgaaaatt tgtgtgcgcg gcgcaacctg gctgcggaac atgcggatct
     4741 catacagctc gttattgatc gtcgcctgaa taatcccctg atgctgctcc gccatggtag
     4801 tgatgttttg cagattcaaa tgcggcagca aatgatcggc aattttgtta cttatgactg
     4861 tgcggttcga ttcctgatcg tgaaccagca ggccgagcgg cagcagtgag actatctctt
     4921 cattaattgc ccgtaaaatg cgcaattcgt tattgaccgc cgtgctgggg acgctttctg
     4981 tactgcggct ggagaaatgg cggaatgtgg tatagccaaa taatgccagc gccagcaaac
     5041 cgatgttcag cagcagtggc agcagaatgt tttgcaacgt atccagcagt aaggtgccgt
     5101 aaggaacctg ccagaccagg cgcatatcgg tagagttgag tgccgaggag atttcaatct
     5161 tcgtactgtt aaagtggata ctgacgctat ccgtcccttc tttctcattt tcattgtttc
     5221 ccgttgccgt cgcgtctggc tcaaggcgga aactgtccag cggcataccc ggtgggatca
     5281 aatcattaat cggcagatca aaagccacga ccgttgccag atgtcccggc tggttgaacg
     5341 tggtacgcaa ggtaaagtaa tgaccgttct gccaggccag gcggcgcatg ttagaaaaac
     5401 tttcgcgttc atcgagggcg ttggcctgtt gcaacatctc tgcacgacgt gaatcaacaa
     5461 tgtcgctgac ggtcgattct ttaaatccgg aggtgagatc tttgaggggc agggttgaga
     5521 tcagcaccag actgttatcc tgaccattca ggtaatacat cgaccacggt acattttctg
     5581 cgccccacaa tgtgtccaga taggtggaca tccgttgagt catctcaagc gttgagttgt
     5641 cgtgagagcc aaagattaac gcttccgttt tgcggcgtgg tttctccaga tagtagacat
     5701 cctgtttcag tcgcgtctct tgtaaacctt cgccggagga gggggaggtc gtcgcggcaa
     5761 tgttgtcgta gatctgccag gtcacgtaac gccaggtatc gacgcgcttt tggattgcgt
     5821 gggtaatgtc gacaatctgg taacttttat ctttcagcca ggcgttaacg gcgctttgta
     5881 ccattacacc catcgtcacc agtaacacaa tgatcaacag taaaaagaag cgggtaatgc
     5941 tccccggtag gagtgaaaag cgggtcgtgg ccgttgtctc tttctgacgc attcgtgttt
     6001 atgacctgtt aaaacttcgc gaagtgatag ggcagtataa agggtacagt gtgaaattcc
     6061 agactcttac ctgattgatg attctctatt ctccagtgat atttatcatc ctgcattggg
     6121 ggaaatagat gtacaagcgc cattttgctg tacaaataac ctacaaaaag cctaacgaaa
     6181 ttaattgcaa ataaacgaag attgctggaa attatgctga tgttaattat ttgtgaaata
     6241 gttaacaagc gttatagttt ttctgtggta gcacagaata atgaaaagtg tgtaaagaag
     6301 ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg ggtaaac
//
LOCUS       DAZKHK010000199         6337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKHK010000199 DAZKHK010000000
VERSION     DAZKHK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.200"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(433..1290)
                     /gene="sitD"
                     /locus_tag="ACHOS5_004724"
     CDS             complement(433..1290)
                     /gene="sitD"
                     /locus_tag="ACHOS5_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFP9779416.1"
                     /translation="MMALLLEPLQFTFISHALLISLVISIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     YIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVAFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1287..2144)
                     /gene="sitC"
                     /locus_tag="ACHOS5_004725"
     CDS             complement(1287..2144)
                     /gene="sitC"
                     /locus_tag="ACHOS5_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFP9779417.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2141..2968)
                     /gene="sitB"
                     /locus_tag="ACHOS5_004726"
     CDS             complement(2141..2968)
                     /gene="sitB"
                     /locus_tag="ACHOS5_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFP9779418.1"
                     /translation="MMQSAGIVVNDVAVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(2968..3882)
                     /gene="sitA"
                     /locus_tag="ACHOS5_004727"
     CDS             complement(2968..3882)
                     /gene="sitA"
                     /locus_tag="ACHOS5_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFP9779419.1"
                     /translation="MHSIKKVTMLLGGLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            4302..4754
                     /locus_tag="ACHOS5_004728"
     CDS             4302..4754
                     /locus_tag="ACHOS5_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFP9779420.1"
                     /translation="MNYFIRPATPDDFNKIIEFTTQEAQESEGSVIDLVAVERGVSSA
                     FSENPRSKYWVMEDSSGTVIANTSVVKEWSDFHGNDYWWIQSFYIAPEHRGMGLADEL
                     IKHLIKEAKSEKALELRLYVHGDNGRAIRAYERCGFIESPYTIMKVGL"
     gene            5009..5593
                     /locus_tag="ACHOS5_004729"
     CDS             5009..5593
                     /locus_tag="ACHOS5_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9779421.1"
                     /translation="MDKRAKNQIVDSDIARLLLKLRKSRNLTVTELAQRSGVSQAMIS
                     KVERGTSSPSATILSRLANALNITLSKLFAELEMQQNSLVLLADQQQHWIDEETGITR
                     WSLSPAGACPELIKVEIPPMGQLTIPASANEHLSGQTLWMLSGSLDFRVNHQTHHLQA
                     GDCLALTFASEYQMRNPDSGQSCSYIVAFSQKNP"
     gene            complement(5618..6055)
                     /locus_tag="ACHOS5_004730"
     CDS             complement(5618..6055)
                     /locus_tag="ACHOS5_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9779422.1"
                     /translation="MLKIRMSRPSEAPEIIQIWKNSVDATHDFLTAHDRQEIEKEVVS
                     FFSETPVLVATNQEDQPLGFMFLHEGHLEALFVDASARGLGVGKHLISHALALHPDLS
                     VDVNEQNHQAVGFYQHMGFKLSGRSERDNQGRPYPLLHLYKAM"
BASE COUNT         1750 a         1483 c         1457 g         1647 t
ORIGIN      
        1 cttactttgt attttcattt cggaatactg ttaagtaact ttaataatcc taacaataga
       61 tcggattttt cttagagtca atggtccccg tttttgcttg caaagcaaat gttttggtag
      121 ttataagttg tcttgatata gtcatgcatg caacttgttc cgtgaagaga ttaaagattg
      181 gaatataacg cgaaaattgc tcatagttca ccaatacagc tataaatcgt aaagagaacc
      241 gtggctcgtt gtatgcaaaa acgtgccacg gttggctgat gcactttcga gagtgcatgt
      301 attgaattat ttccagctat tatcgatttt acgtattttt tgcatgatag aattcgtacc
      361 tcctcccaac gaccttccat gactttccgg actgctgtag gctggcggac gatgatgtcc
      421 tcggatgttg tgctaattgg gtgataacag atcctgtgta tgggcgtttc cctttgtgcg
      481 tgcgttgatg ctggtaacgg taaacgtcat gatgaacgcg accgcgaata agacgacaat
      541 cgtcggagct ggtgcgctgt cgataaaaaa ggacagccag acaccggtca ttgacactat
      601 taccgatatg ccagtcgcca gtaggagcgc aatataaaag cgttgtgtca ggagtaccgc
      661 aatggcgcca ggcgcaatga gcagagaaat ggagagaatg atacctaccg ctttcagcgt
      721 cgccacaatg gtcagagaga ccatgcacag cagcccataa tgcagccatc tcgtatgcaa
      781 accgcttacc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatctc gccattttac
      841 gctaataaca agtgtaacca gcccagcaat aatcaccgtc tggattatat cgccgatggt
      901 tatcccgagc atgtcgccga aaagaatatg gtcaagatgt acgtctggtt tgactgcaat
      961 atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccatca ctgtatcttg
     1021 cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcgcagaata ctccggcgac
     1081 gaatgcccct gtagccaaag gtaaccccag aatccaggca agcacgatgc caggaaaaac
     1141 cgcatgactc atcgcgtcac ccatcagtgc ccaacctttc aggaccagaa agaccgacag
     1201 cagcgcacag ggtatggaaa ttaccagtga aatcagcagt gcgtgactta taaaggtgaa
     1261 ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcctt atgcgcacgc
     1321 cgacgatttg ccaaaagccc atgggtgggc gcaaagacaa atgctaaaag aaacagcagt
     1381 gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact gacccacgcg
     1441 ccgaggaaac tggttacact gccaatagtt acagcaatca tgagcaggcg gggaaaacga
     1501 tcggttaaca accatgctgt tgctccggga gtcaccacca gacagatcac cagaaaggca
     1561 ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa gaggagtttt
     1621 aatctgcccg gatgcaatcc aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata
     1681 agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat agtgagttgc
     1741 aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg aatgttcaca
     1801 gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga gaagatcagg
     1861 ccgatgatgg catcttcttt taagcgggtg cgttggttaa gaaacagcat gctgcccgca
     1921 gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat atatgccccc
     1981 gcaacacccg ggacaataga gtgcgacaac gcatcgccaa tcagcgacca gccttttagc
     2041 atcagatagc atgacaggaa agcgcagaga ccgccgacca tcgccgagac ccacatcgca
     2101 ttgagcatat attcatagct gaagggttcc agtagcacgt tcatctttct tccctctgca
     2161 ccgctgacgg tcgatgagcc acaaaagggc gttcatcgtc agtaataatg ctttcttccg
     2221 agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg ttctcggctg
     2281 taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg accgtataat
     2341 cgcaaaatgt cgtgacggat ccgagattgt gcgttgagac aagcatcgtt ttgccttccg
     2401 cgcgtaactc cctcaacagg ctgatgattt tggcttcggt tttcacatca acgccagtaa
     2461 aaggttcatc cagcaggatc acatcaccct gctgtgcaat cgctctcgcc agaaagacgc
     2521 gtttcttttg tccaccggaa agctcgccga tttgtcgatg gcgaaaattc accatatcga
     2581 cacgttccag cgcatcggtg acaatttgac gatccctctt tttggcgata cgcaacatac
     2641 ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga aatgaccagt
     2701 caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtaacgcc tgtcgcgtgg
     2761 gaatacccag aacagatatc tttccgctgg tcagacgcac aaatcccata atcgctttaa
     2821 acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat ccacttggta
     2881 ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca gcaacatcat
     2941 taacgacaat gcctgcagat tgcatcatta tttctccctt ttcccggctt taattccctg
     3001 taccagcgta ctggtagtaa ccttaagaag gtcgatatac gtcggtaccg ggccgttttc
     3061 tgtgctcagg gaatcgacat agagcacacc gccgtagtgt gcgccggttt cacgcgcaac
     3121 ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga tatgattttt
     3181 cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat cggcattaat
     3241 cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa aagccccttc
     3301 actggtgacc atccatcgct gattctcagg gagttccgta atctgcttac gcaggggggc
     3361 aagggtttgg gtaatcttgg ctttataagt atcggcattg cgttggtagg tttgtgcatt
     3421 tgccggatcg tattttatca acgcatcacg aatattatcg acgtaaatca gagcattatc
     3481 tggcgacatc catgcatggg ggttaggttt gccctcatag ggtccttcgg tgatccctac
     3541 aggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct ggtaaaagcg
     3601 ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc cctgcgcacg
     3661 tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct tggttatgga
     3721 tgagacttct gcagcatctc ccgccacgtt tttggccata tccgcgatga tggtaaatgt
     3781 tgtaatgacc ttgaattttt cagttgcact tgcctgaaat gcgatcgagc aggtgagtgc
     3841 gaggcccccc aagagcatgg ttactttttt tatcgagtgc ataatttagt acctatagtg
     3901 agcaaaatta gaacaaacat agcaacggct atgttttata gcacaaccta tacaaaatga
     3961 aaatgattat cattagcaac tttgaggccc aaaacaggga ttaggttgtt tcctgctggc
     4021 gacgtgttat tgaggaaaaa agtaaggcag ctagtaggct ttgaaataac gttctgcggt
     4081 tggctgatgc aatttcgaga gtgcatgtat tgaatgattt ctagccgtta ctgattttcg
     4141 tattttttgc atgagatgat ttgaacgtcc tcgcaccaac cttccatgat tgctcgtcat
     4201 tgtctctagg actgctatgt gccaggagcg gacttttaac aggatttgat gtacggtatt
     4261 gggcgattcg tatagcagca acgaaaatga gagccagact tatgaactac tttattcggc
     4321 cagctacgcc tgacgatttt aataaaatca tcgaattcac cactcaggaa gcgcaagaat
     4381 cagaaggttc agtgattgac cttgttgctg tggagcgtgg tgtatcgtcg gcgttcagtg
     4441 aaaaccctcg ttcaaaatat tgggtcatgg aagattcttc aggaacggta attgccaata
     4501 cctctgtcgt aaaagaatgg agtgatttcc atggtaatga ctactggtgg attcaaagct
     4561 tttatatcgc cccggagcat cgagggatgg gactggctga tgagctcatc aaacatctta
     4621 taaaagaagc caaatcagaa aaggcacttg agcttcgctt atatgtccat ggagataacg
     4681 ggcgtgcgat tcgtgcttat gagcgatgtg gtttcattga atctccgtat accattatga
     4741 aagtgggttt ataacttccc caaatacagc cctcttaatg ctctacctga aatcaggaca
     4801 caatgccgca agcggcatct ttcattcaaa tgatgttctt gaacgttcta ggcacataac
     4861 gacaacttcc gctcctcgct cataacgggc gttcatttat ggcaaaaagg cttaagttta
     4921 gaaaacatgc gttttagacg taaaaaatca tttatttaat cacaacttat gtaatggcat
     4981 aatcaggttg gtgaaagaga gggcgtaaat ggataaaaga gctaaaaatc agatagtcga
     5041 tagcgatatt gcacgtttgt tactcaagct cagaaagtct cgaaacttga ctgttacaga
     5101 gcttgcccaa cgatctggtg tcagtcaggc gatgatcagc aaagttgaac gaggcacctc
     5161 aagccccagt gcaacaatac ttagtcggtt agctaatgca ctgaatatta cgctatcaaa
     5221 gctttttgca gagcttgaga tgcaacagaa ttcacttgta cttttggctg accagcaaca
     5281 acattggatt gatgaagaaa caggcattac ccgctggtct ttgtccccag cgggtgcatg
     5341 tccggaacta ataaaagttg aaatcccccc tatggggcaa ttgactatcc ctgcctctgc
     5401 aaatgagcat ctttctggac aaactctatg gatgttaagc ggcagccttg attttcgagt
     5461 caatcatcag actcatcatt tacaagccgg tgattgtctt gctcttacct ttgccagcga
     5521 gtaccaaatg agaaacccag atagcggcca gagttgttct tacattgtcg ctttcagtca
     5581 aaaaaatcca tgatgtaatt ctcctaaaat aatcatgtca cattgcctta taaagatgta
     5641 atagagggta gggtctgcct tgattgtccc gctcggaacg tcctgaaagt ttaaagccca
     5701 tatgttgata aaaacctaca gcctgatgat tttgttcatt tacatcaacg cttagatcag
     5761 gatgtagagc cagcgcatgt gagatcagat gtttcccgac gccaagtccg cgagctgacg
     5821 catcaacaaa aagagcctct aaatgtccct catgcaaaaa catgaatcct agtggctgat
     5881 cttcctggtt tgttgctacc agcacagggg tttctgaaaa aaagctgaca acttcttttt
     5941 caatctcctg ccggtcatgg gctgtaagaa aatcgtgtgt ggcatcaacg gaatttttcc
     6001 aaatttggat aatttctggt gcttcagatg gtcgcgacat tctgattttt aacatgttat
     6061 ctcccttaaa gtggtcgttg cttgtgtctt gagatacaaa acgagctatc aaatttacta
     6121 tagtgaaatt aaattcacaa tagtgatttg gttcctgtgc cttatcatta cttatgagga
     6181 gtacaattaa acgggttaat cagtttctcc atttgaaaga aaaagtcttc tgtactgttt
     6241 gatatcaccg ctattttctt atttgctgtg caggtcgtag tgggacaaaa ctgagacaca
     6301 taaggtctag caatggcttg caaggcctaa catgtat
//
LOCUS       DAZKHK010000200         6289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKHK010000200 DAZKHK010000000
VERSION     DAZKHK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.201"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..382
                     /locus_tag="ACHOS5_004731"
     CDS             80..382
                     /locus_tag="ACHOS5_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP9779423.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNVKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            385..744
                     /locus_tag="ACHOS5_004732"
     CDS             385..744
                     /locus_tag="ACHOS5_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779424.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(791..1183)
                     /locus_tag="ACHOS5_004733"
     CDS             complement(791..1183)
                     /locus_tag="ACHOS5_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779425.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1310..1570
                     /locus_tag="ACHOS5_004734"
     CDS             1310..1570
                     /locus_tag="ACHOS5_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9779426.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1567..2004
                     /locus_tag="ACHOS5_004735"
     CDS             1567..2004
                     /locus_tag="ACHOS5_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9779427.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            2091..3101
                     /locus_tag="ACHOS5_004736"
     CDS             2091..3101
                     /locus_tag="ACHOS5_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFP9779428.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEVYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSELHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAIQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWR
                     DD"
     gene            3094..3555
                     /locus_tag="ACHOS5_004737"
     CDS             3094..3555
                     /locus_tag="ACHOS5_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9779429.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISGSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            3589..4305
                     /locus_tag="ACHOS5_004738"
     CDS             3589..4305
                     /locus_tag="ACHOS5_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9779430.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLIKAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPVADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEDIKTETVADIVQSLPSFTETRADDLVLPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDILRDITATRELLR"
     gene            4338..4619
                     /locus_tag="ACHOS5_004739"
     CDS             4338..4619
                     /locus_tag="ACHOS5_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9779431.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            4616..4921
                     /locus_tag="ACHOS5_004740"
     CDS             4616..4921
                     /locus_tag="ACHOS5_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9779432.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKQEGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            4908..5510
                     /locus_tag="ACHOS5_004741"
     CDS             4908..5510
                     /locus_tag="ACHOS5_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9779433.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLADVRAEVFND
                     LCAAFVRHAKVAGLDDADTVTFKEATEALLHCAEQLRAPE"
     gene            5606..5788
                     /locus_tag="ACHOS5_004742"
     CDS             5606..5788
                     /locus_tag="ACHOS5_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779434.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            5781..5966
                     /locus_tag="ACHOS5_004743"
     CDS             5781..5966
                     /locus_tag="ACHOS5_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779435.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGEDDED"
     gene            6072..6275
                     /locus_tag="ACHOS5_004744"
     CDS             6072..6275
                     /locus_tag="ACHOS5_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779436.1"
                     /translation="MTIVKTHTGIVITKDGPQVKKLHQTERMWVVDKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPVEVKHV"
BASE COUNT         1775 a         1323 c         1740 g         1451 t
ORIGIN      
        1 taactggtca acatgtttat tattaaattt gtaattgcga gctgttgcat tagtcccaaa
       61 atgggactac tatacagtta tgaagattat ctcagttaaa acactcaggg atttttgggc
      121 ggagaatcct gatgcagaac aaccgctaag ggcatgggtg gatgaggcgg caaaagctga
      181 ctggaaaagc ccagcagaca ttaaggcaca ataccgaacg gccagtatat tgaaaaaccg
      241 gcgcgtggta ttcaacgtaa aaggtaatca ttaccgttta attgttgcaa ttgcgtatca
      301 gcgaggatgg gtatttgtta aattcatcgg aaaccacaag gaatacgatg ctattgatgc
      361 tgataccatc gaactggagt aagcatgaac atcaaaccta ttcgtacaga gcaagattat
      421 gaagccgcgt tgcgtgctgt tgaacccatg ttcgacaatg agcccgaaat tgacacgcct
      481 gagggggatt tttttgaggt gatgtgtttg ctaatagagg aatatgaaaa aaaacattat
      541 cccattgagc caccatcccc aattgaagct ataagatttc gcatggaaca gcaggggctg
      601 actgtgaaag atttggaacc cgcaattggg aaaaaaaatc gggtttatga ggtgttgaat
      661 ggtaccagaa gccttacgtt accaatgatt cgccgtcttc ataatcaatt tggtatcccc
      721 ctggaaagcc tggttggatt ataaaatctg ctagtcattt gcctgatgct cgttccagaa
      781 aaggaatgca tcaggcagtt ttgtttttct gccgcagtaa ctcttcaagt ttccgtttat
      841 agaaatcgcg tttttgctcc atatcacgaa tgatctgctc tgcgtcgctt tgaggtaact
      901 catctaaaag cgatatgatt tttcgttgtt gttctgtaag ttgcggttgg ttgtcattac
      961 tggatacagc cattttatcg ccgagagttt cttcttccat aaagaaccaa tggacgggat
     1021 gttgtgagag ctctgctaat ttttccagtt tatccattct tggcatcacg cctttcaacc
     1081 aaccttgcac ggattggggt tttacaccaa gacgtcttcc cagctctgac tggtttatat
     1141 tcaattcctg caacacctgc tgaaggcgtt ttacaaagat catcaccacc cctcgtaaac
     1201 tagttccgcg atcctacaga aaaaattgat aagtggcatt acaaatagaa gttgaaattt
     1261 aaatttaaat ttgtaatcat cggtgtcatc gtaaagttcg gagggaaaca tgcaaaaaag
     1321 tactcaagtg aaaatcctgt caataatgag ccaatcagaa ttagggcgtc gtcttggtaa
     1381 aacaccgcaa accataagtg ggtggtttaa aaaacgagtg ccagcggagg aggttatacc
     1441 agcatgtgag gcgcttgact ggggagtaac tccgcatgaa ttgcgccctg ataaataccc
     1501 taatccaacc gatggtttac ctgttgagta tcaggctaac gcacaagcag cggcgggagt
     1561 tgattcatga aaatcaagca tgagcacatc cgcatggcga tgaatgcgtg gctgctttat
     1621 ccgagggtag ggcgcaaaaa aatcgctgat gatatagcga cagcatattt tgagcttgaa
     1681 atgacttatc caccaatgca tgacacctct acgacagagg gtattggatt gaatatacaa
     1741 aatatttttc gctggcttga aaaggatacg cctgatgctg ttgaaaaaat tcaggcacta
     1801 attccggcta tcctgactgt tcttccgcgt gaactgcgtt atcacctcag tatttttgac
     1861 actgttgagc gccgtgcatt actggcggcg caggaagcgt tgagcacggc aattgatgca
     1921 catgatgatg ctgtccaggc cgtttaccgg aaagcgtatt tcagcgacgg cgggtcatcc
     1981 ggcgagtctg ttgtggtgca ttgatattta tgccagcccc ctgctgattc tgttgattgg
     2041 ggaatcacag aatatcacca gaggatggtt cgtcacaaga tgaggcaatt atggccgcat
     2101 taccatacat gcaactgtac atagctgatt acctggctga caccatgcat ttgtccgcag
     2161 aggagcacgg tgcgtatttg ttgctgatgt tcaattactg gcaaacaggg aaaccaatac
     2221 ctaaaaacag gctggcaaaa atttcccgtc tgactaacga gcgatgggct gatgtggaac
     2281 catccttgcg ggagtttttt tgcgataacg gcgatgaatg ggtgcatctt cggattgagg
     2341 aagatctggc atcagtcagg gaaaaattga ccaaaaaatc agccgccgga aaagcatctg
     2401 ttcaggccag aagaagcaga aaggaagtat atgttcaaac aaaacaagag agagatttaa
     2461 caggtgttca aacaaatgtt ggtgttgtgt ttgaacatga cgcaaacaca aaagcaacta
     2521 ataaagatac agatctaaaa gaattaaacc ccacacataa cgtgcgtgaa cgcgagagta
     2581 ttccgaccag tgagttgcat ggtgcgccgt tgcagacagc cgaacctgaa tacctggacg
     2641 gcctgagcga accgatcggg aaattttcga tgaccactgt ctggcagccg tcgccggatt
     2701 ttcgacaacg ggcagcagtg tggggtatgg ctctgcctga gccggaattt acacctgctg
     2761 agcttgccgc attccgggat tactggatgg cggaggggaa ggttttcaca caggttcagt
     2821 gggagcagaa atttgcccgc cacgtgcagc acgtcagggc acaggtaaaa ccagtcagca
     2881 agggggtaag ccatgcagca tcaggtggca cggcatcacg ggcaattcag gaaatccggg
     2941 cagcacgcga acagtgggaa cgagacaacg gatttatcag caacggaaac ggcctggaag
     3001 ctgtgggagc ttatggggga ggtgtattcg aaccgctgga ctcagaagaa cggggccgca
     3061 ccttcgaagc tctggattgc ccagattggc gcgatgactg aacagcaaat ccggctggtc
     3121 tgccgtcagt gcatggaccg ctgccgggcg ggtgaaacgt ggcccccgga cctggctgag
     3181 tttgttgcgc tgatttcggg gagtggggca aatccatttg gtcttacggt ggatgccgtg
     3241 atggaagagt accggcgctg gcgcaatgaa tcctggcgat acgacgggag tgataaatac
     3301 ccgtggccac agcctgtgct gtaccacatc tgcctcgaaa tgcgtaccag agggattgag
     3361 cgccagatga cgcagggtga gttaaaacga cttgcggaac ggcaactgac gaaatgggca
     3421 aagcatgttg gtaacgggat gagtgttccg ccagtgcgac gacaactgga aggggcgaaa
     3481 cacccgcaag ggccaacgcc aattgaacgg ctgaaacagg aatacgaacg ccggaaggca
     3541 gctggtttta tttgaatctg agaaacgatt ttgtcggagg aaattttaat ggaaaccgta
     3601 tttgacgcac tgaaagcact gaaaaaagcc tcttcacagg tagtggcatc gcgccttgga
     3661 atcagccgcg aagatgcggt caacgaactg tggaaactga agcgccgcgg tgaagcggat
     3721 aacaagggtt cgatgtggtg gctgattcag gctggtgaaa gtgaaccagt gtcaccggta
     3781 ccgaaagtga cagcgcaaat gctgattaag gcgattgaac aacatggccc acaaacggcg
     3841 gatgagctgg cactgatgtt cgggattacc tcccgccggg cgaattcatc gctggccatg
     3901 gcaatcagca aagggcgtct gattcgcgtg aatcagggcg gtaaatttcg ttactgcata
     3961 ccggtcgctg atttaccggc agagccggaa gctgcatccg tagcggaaac ggatggtaaa
     4021 gcctttcctc agccagcagg tgttgcgtta ccagtccggg aagcggaaac acaggaagat
     4081 attaaaacag aaactgtggc tgatattgtg caatcgttgc catcgtttac tgaaacgcga
     4141 gcggatgacc tggttttacc atcgctgcat gtggctaacc gcgagctgcg ccgggcaaaa
     4201 ggtcaggttc agaagtggga gcgagtctgt gccgcgctgc gggaactgaa caaacacagg
     4261 gatattctcc gggatattac cgccaccaga gagctgctgc ggtgagtggc tggaagaagt
     4321 ggcgctgggc ggaaattatg atactccggc agtgtgcggg aacgatgaca gtcgaaagca
     4381 tcggcagtct gattggccgt agtggggctg ccgtcaggac gaaagcgcgg gaactgggta
     4441 tctgcatgat gttacggggt gattatcacc agtcagccaa atgttcacag cgtgatattg
     4501 agctggcgcg gcaactgcat cagcgtggcg taccccgacg ggaaattgcc gaaaagtttg
     4561 ggatgaagtt gggcgcagtg aataactacg tttatttcga caggagggtt caggcgtgag
     4621 ggtgagaatt tatatcgccg gtccgatgac gggatatgaa aatttcaacc gtgaggcgtt
     4681 tcacaaggcg gaagaagcac tgaaacagga agggcatacc gtattaaatc ccgcagtact
     4741 tccggacggg ctgactcagc cacattacat ggatatttgt atggcaatga ttcgttgtgt
     4801 ggatgcggtt tatatgctga aaggctggca gcggtcggca ggcgctaagg cagaactggc
     4861 actggcggag aaactggggc atgcagttat tttccaggag gcaaccagtg agaaaaatta
     4921 attatcagac gctacgtgag atagcaaaac tggcaaccca gggcgaatgg gtcgcattca
     4981 tttcgccggg cactgataca tatgcggtgc atacgccggg ggatgaacgt tgcggagaca
     5041 ttatcaaatg gcctggcttt gatgaccaga aaaatgcaga gaacaacgca gaatttattg
     5101 cagctttcaa ccctgaagta gtacaggcac tgctggatga acgggaagcc caaagcaaac
     5161 gtattgccga gctggaagct aatcttgtgg cgctggctgc ggagaatgca cttgctcgta
     5221 aagcagttca ggcattctgc gatgttgttg gcgacaacac cgaggttatt tgcgaggagg
     5281 ttgggagaga tggcgttctg gttattttgg aggccatgaa ggcaacagga aatatgccag
     5341 ccaccgatgc tttcctggct gatgtacgag cggaggtgtt taacgacctt tgcgcggcgt
     5401 ttgtcaggca cgcaaaagtt gcaggactgg atgatgccga taccgtaacg tttaaggaag
     5461 cgacagaagc attgctgcat tgtgcagaac aacttcgcgc acctgaataa ttaaatttag
     5521 cactgtaaat aaaatttaat ccttaaccgg agggattcct gcaccctcag aacatcagga
     5581 gaccgcccgg cagggcggta gtgaaatgcg aaagttcaaa ataattattg aaacgggaat
     5641 agccggtgga gattttgagg atgtattcga agtggacgat gacgcaacac ctgatgaaat
     5701 tcatgacgaa gcaaaagaaa ttttctttaa ctactgcaat tactcatatc acgaaataaa
     5761 agacgaagag gaagaacaaa atggctgatt ttggttcaac taaatataac gccagttttg
     5821 aagaatggca tgaactgtta atggactatg cagagttacg cggtggaagt gccgccgatg
     5881 ctgaagcatg gcgtgatgac tatgaagcag gaaaaactcc ggttgaagct tattgtgatg
     5941 agtggggtga ggacgatgaa gattaactat caggcgatgg tgcaggccag gcgattcgat
     6001 accggctcgt gttcagagtc atgtgaaatc tggggtgaac aattggaatt gaaattcgaa
     6061 gaggtggtgg catgacaatc gtaaaaaccc ataccggcat cgtgatcacc aaagacggtc
     6121 cgcaggtaaa aaaactgcac cagacggagc ggatgtgggt cgtcgacaaa aacgagtttt
     6181 accacaaaga aaccggacgc cgccactttg cagaaaatac gcgccgccga ctactgatcg
     6241 acaccatcaa gcctgtcgag gtgaagcatg tttaaacaga acgaaaaat
//
LOCUS       DAZKHK010000201         6276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZKHK010000201 DAZKHK010000000
VERSION     DAZKHK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.202"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(14..1144)
                     /locus_tag="ACHOS5_004745"
     CDS             complement(14..1144)
                     /locus_tag="ACHOS5_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9779437.1"
                     /translation="MRIHTYWYRRYFILLIIIFSNVLSSIANAEDMGRERAYCYPGSP
                     SNNTTPASFSYNFGTIVVSDVNKNAPGTVLPSQIWKVGTYKAYCNSLDDYEIYFSAVS
                     GIDPSGASGDHQGSDVFIPLTHEISVSTHIKLYNQNGTMTDKIVPFENYNTNYPGDRS
                     KPSNWASGTEGYIKIRIDKKIISDVSLSNVLLVSLYVSQIPTEHGPIPVFNAYIGNLN
                     IQVPQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDIDAV
                     MTLVFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNSVDLNALATSVGVPFRLV
                     ENQAASAQTADVTFLTLPVSTTGKVPAAGRYNALAILRVEYQ"
     gene            complement(1163..1939)
                     /locus_tag="ACHOS5_004746"
     CDS             complement(1163..1939)
                     /locus_tag="ACHOS5_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9779438.1"
                     /translation="MRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSS
                     ISVELKNTSDRDMLVKVAVSPDDARQLTGTVESLPSYSETVFIATPPLFILKPDKNTK
                     IRINRVGGSLPADRESLFILNVAALPTLENSHSVKTDNQLQIAVRNRMKIFYRPSNLS
                     EDPNMSYQKLRWARKNEIVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCK
                     SQGVCEIAWQTLDIYNNVLPILKVKLNPSQLDVTGFAFSQ"
     gene            complement(1905..2477)
                     /locus_tag="ACHOS5_004747"
     CDS             complement(1905..2477)
                     /locus_tag="ACHOS5_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9779439.1"
                     /translation="MPGKQMLCCILISIISEGDMKIFISLFLFIISTNSFADDITHAG
                     VVRIEGLITEKTCIISDESKNFTVNMPDVPSSSVRSAGDVTEKVYFSITLTRCGSDVG
                     NAYIKFTGNTVSEDASLYKLEDGSVEGLALTIFDKNKGSISNDVKSMGFSLTSSVDNI
                     LHFFAAYKALKNNVQPGDANASVSFIVTYD"
     gene            complement(2428..2991)
                     /locus_tag="ACHOS5_004748"
     CDS             complement(2428..2991)
                     /locus_tag="ACHOS5_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9779440.1"
                     /translation="MNLIFSKYSVFFIFFAFFAFPLSGQANDSVVVTFTGILLAKSCD
                     ITTGSKDQSVKMGTYDANEFLNVGDVSPSQTFTINVQGCPTAKSTVYSSGVSANVRFT
                     GDADAINSALLRLSAGADSATGLGIEILDNNDVAIAINGESGFRDLVLNENGDANLTF
                     KLRYKSTQENVHAGQANALLYFDIDYQ"
     gene            complement(2991..5585)
                     /locus_tag="ACHOS5_004749"
     CDS             complement(2991..5585)
                     /locus_tag="ACHOS5_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9779441.1"
                     /translation="MNLKLKRCEYWMAAQKQMKRVVPLLLVIMPACSIAGMRFNPAFL
                     SGDTEAVADLSRFEKGMTYLPGSYEVEVWVNDSPLLSRTVTFKADDENQLIPCLSLAD
                     LLSLGINKNALPEQALASSENSCLDLRIWFPDVHYMPELDAQRLKLTFPQAIIKRDAR
                     GYIPPEQWDNGITAFLLNYDFSGNNDRGDYSSNNYYLNLRAGINIGAWRFRDYSTWSR
                     GSNSAGKLEHISSTLQRVIIPFRSELTLGDTWSSSDVFDSVSIRGIKLESDENMLPDS
                     QSGFAPTVRGIAKSRAQVTIKQNGYVIYQTYMPPGPFEISDLNPTSSAGDLEVTIKES
                     DNSETVYTVPYAAVPILQREGHLKYSTTVGQYRSNSYNQKSPYVFQGELIWGLPWDIT
                     AYGGAQFSEDYRALALGLGLNLGVFGATSFDVTQANSSLVDGSKHQGQSYRFLYSKSL
                     VQTGTAFHIIGYRYSTQGFYTLSDTTYQQMSGTVVDPKTLDDKDYVYNWNDFYNLRYS
                     KRGKFQASVSQPFGNYGSMYLSASQQTYWNTDKKDSLYQVGYNTSIKGIYLNVAWNYS
                     KSPGTNADKIVSLNVSLPISNWLSSTNDGRSSSNAMTATYGYSQDNHGQVNQYTGVSG
                     SLLEQHNLSYNIQHGFANQDNSSSGSVGVNYRGAYGSLNSAYSYDNEGNQQINYGISG
                     ALVVHENGLTLSQPLGETNVLIKAPGANNVDVQRGTGISTDWRGYAVVPYATEYRRNN
                     ISLDPMSMNMHTELDITSTEVIPGKGALVRAEFAAHIGIRGLFTVRYRNKSVPFGATA
                     SAQIKNSSQITGIVGDNGQLYLSGLPLEGVINIQWGDGVQQKCQANYKLPETELDNPV
                     SYATLECR"
     gene            complement(5622..>6276)
                     /locus_tag="ACHOS5_004750"
     CDS             complement(5622..>6276)
                     /locus_tag="ACHOS5_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9779442.1"
                     /translation="SILFINNASAGGFGLGVTRLIYPAESKQITLSVQNSGDNTSYLI
                     QSWVDDSKTEKKSQDFVITPPLFMLPTRKEASLRIMFLGKTNLPTDRETLYWMNVKAI
                     PPTDEKNTQKNTLQLALQNKIKLFYRPENLPVQPGKARNMLRFKYEGKQLKVINPSPY
                     YLTLTGIKVQGSKLPNMFVPPKSDITVSSPVTLAGEITYQTINDFGATTERQKGIMQ"
BASE COUNT         1913 a         1370 c         1143 g         1850 t
ORIGIN      
        1 actggctcaa aaattactga tattccactc gcaatatagc cagtgcatta taacgacctg
       61 ccgcaggcac ttttccagtt gtactaacag gtaaagtcag gaaagtaaca tcagcagttt
      121 gtgcagaagc agcctggttt tccaccaatc tgaacggaac gcctactgat gttgccaggg
      181 catttaaatc tacggagttt tgctgactat ccataatcat aataccaaca tcaggattat
      241 cttgagcctg aataatactt tgtttcccat tggtatcacg tgtggcggaa atgttaccgt
      301 cgaataccaa cgtcattacc gcatctatat ctttatttgt acaattaatc ggaatagttg
      361 ttgctacagg agtaacacca gcgggcttcg ctccggcacc agcccggctc aattcactgg
      421 cccacacatc cggcatatta acagtaaaac tcgtaccctc attaatagtg caaccttgcg
      481 gaacctgaat atttaagttt cctatgtagg cattaaagac agggatagga ccatgttcgg
      541 tagggatctg gctgacatat aatgacacca ataatacgtt acttaatgaa acatcagata
      601 taattttttt atcaatcctg attttaatat atccttcagt acctgatgcc caattagatg
      661 gtttgcttct gtcccccgga taattggtat tataattttc gaatggcaca attttatctg
      721 tcattgtgcc attttgatta taaagtttta tatgagtaga gacagatatt tcatgggtga
      781 gtggaataaa tacatcactc ccttgatgat caccactggc accagacgga tctattccag
      841 agacagcact gaagtaaatt tcataatcat caagagaatt acaataagcc ttataggttc
      901 caaccttcca gatttgtgat ggcaatacag tgccaggcgc atttttgttg acatcagaaa
      961 ccactatagt accaaaatta taagaaaagg atgcaggcgt agtattatta ctcggtgaac
     1021 ccggataaca atatgcacgt tctcgcccca tatcttcagc attagcaata gaagaaagaa
     1081 catttgaaaa tataataatc aataaaatga aatatcttct ataccaataa gtatgtatac
     1141 gcatatcatg tcctcagata tattattgac taaatgcaaa gccagtaaca tccaactgtg
     1201 aaggatttaa ttttactttc aaaattggca atacattatt ataaatgtcc aatgtctgcc
     1261 atgctatttc acaaacccct tgcgatttac accatgattg ttggcgatgg ctaaatggtg
     1321 cgaccatacc accatctata actttaccat caacatgtag gttatagagt gtcacatatc
     1381 tggggcctgg attataaacg gtaacaattt cattttttcg tgcccagcgt aatttttgat
     1441 atgacatatt cgggtcttca gacaaatttg atggccgata gaatattttc atacgattgc
     1501 ggactgctat ttgcaattga ttatcagttt tgactgaatg actattttcc aacgttggta
     1561 atgctgcaac attcagtata aataaacttt cccggtcggc aggtaaactg ccaccaacac
     1621 gattaattct tatttttgta tttttgtcag gcttcaaaat aaacagtggc ggagttgcga
     1681 taaataccgt ttcagaatat gagggcaaag attcaaccgt tcccgtcaac tgacgagcat
     1741 catcagggga gactgcaacc ttgactaaca tatctctgtc tgaagtgttt ttgagttcaa
     1801 cagatattga ggactgattt tccggatacg caaaacgcgt tccaccgatc actaagcctg
     1861 cttgtgctgt agagaataaa agggaaaata atactatgac taccttaatc ataggtgaca
     1921 ataaacgata ctgacgcatt tgcatcccct ggttggacat tattttttaa tgctttgtaa
     1981 gccgcaaaaa aatgcaatat attatcaact gatgatgtaa gtgaaaaacc catgctttta
     2041 acatcattac taatactgcc tttgttctta tcaaaaatcg taagtgcaag cccctctacc
     2101 gagccatctt ccagcttata taaactggca tcttcagaaa ctgtattgcc ggtaaacttt
     2161 atatacgcgt tgccaacatc actaccacag cgggttaacg ttatggaaaa ataaaccttt
     2221 tcagtaacat cccctgcact ccttaccgaa ctactgggta cgtctggcat attaactgta
     2281 aaattttttg actcatcaga aataatgcag gttttttcgg taattaaccc ttcaatacga
     2341 accactccgg catgagtgat atcatcagca aaagaatttg ttgatattat aaacaaaaat
     2401 aaactaatga atatcttcat atctccctca ctgataatcg atatcaaaat acaacagagc
     2461 atttgcttgc ccggcatgta cattttcctg agttgattta tagcgaagtt taaacgtcaa
     2521 attagcatca ccgttttcat ttaacactaa atcacgaaaa cctgattcac cattaatggc
     2581 aatcgccaca tcattgttat ccaatatttc tattcccagt ccagtcgcac tatctgcacc
     2641 ggcggagaga cgcagtaatg cgctattaat ggcatctgca tcaccagtaa accgcacatt
     2701 ggcgctcaca ccagaactat aaacagtact ttttgcagtc gggcatcctt gtacattaat
     2761 cgtaaacgtt tgactcggag aaacatcacc tacatttaaa aattcattag catcataagt
     2821 gcccattttt accgactgat ctttacttcc ggtagtaatg tcacagcttt ttgccaacaa
     2881 aatcccagta aatgtaacaa caactgaatc attggcctgc cctgagagcg gaaaagcaaa
     2941 aaaagcgaaa aaaataaaaa atacagaata tttagagaat attaaattca ttagcggcac
     3001 tccagagttg catagctaac aggattatcc agttctgttt cagggagctt gtaattagcc
     3061 tgacattttt gctgaacacc gtctccccac tggatattaa taacaccttc taaaggcaat
     3121 cctgagagat aaagttgtcc attatcgccg acaatcccgg taatttgact actgttttta
     3181 atctgagcgc tggctgtagc accgaatggg actgatttat tacgataacg aactgtgaac
     3241 aaaccacgaa taccgatatg agcagcaaac tctgcacgaa ctaacgcacc ttttcccgga
     3301 ataacttcag tggaagtgat atccagttca gtatgcatat tcattgacat aggatctaat
     3361 gaaatattat tacgtctata ttctgttgca taaggaacaa ctgcatatcc acgccagtca
     3421 gtggatattc ctgtcccccg ctgaacatcc acattattcg ctccaggcgc ttttatcaaa
     3481 acattagttt cacctaatgg ttgactcaac gtaagaccat tttcatgtac aacaagagca
     3541 ccactgatgc catagtttat ttgttgatta ccttcattat cgtaactgta ggcggaattc
     3601 aaggaaccat atgccccacg ataattaaca ccaacagaac cactactgct attatcctga
     3661 ttagcaaaac catgttgtat gttataactg agattatgct gctccaacag agaaccagat
     3721 acccccgtat attggtttac ctgtccgtgg ttatcctgac tataaccata cgttgcagtc
     3781 atggcattcg atgatgagcg cccatcattc gtggaagata accaattact tataggtaat
     3841 gagacattta gcgagacaat tttatccgca tttgtccctg gtgatttact gtaattccac
     3901 gcaacattta gatagatacc cttaatactg gtgttataac caacttggta taaagaatct
     3961 tttttatcag tattccagta tgtttgctga ctagccgata aatacataga cccgtagtta
     4021 ccgaaaggtt gcgatacact agcctgaaat tttccacgtt tgctataacg caagttataa
     4081 aaatcattcc agttataaac gtaatcttta tcatctaacg tttttggatc aacaacagtc
     4141 cctgacattt gttggtatgt cgtatcactt aaagtgtaaa agccctgggt tgaataacga
     4201 tagccaataa tatggaatgc tgttcctgtc tgaactaacg atttggaata aagaaaacga
     4261 taagattgcc cttgatgttt gctcccatcc acaagcgaac tgttagcctg agtaacatca
     4321 aacgatgttg caccaaatac acccagattc aggccaaggc cgagcgccaa cgcccggtaa
     4381 tcctcagaga attgtgcccc accataagcc gtaatatccc agggtaaacc ccaaattaat
     4441 tccccctgaa atacataagg acttttctgg ttatagctat tgcttcgata ttggccaacc
     4501 gtagtagaat attttaaatg accttctcgt tgcaggatgg ggacagcggc ataaggtacg
     4561 gtatagacag tttctgaatt atcagactct ttgatggtaa cttccagatc tcccgcagat
     4621 gatgtcgggt taagatcgct aatctcaaac ggtcccggcg gcatataggt ttgataaatg
     4681 acataaccat tctgtttgat tgttacctga gcgcgacttt tcgcaattcc gcgcaccgtg
     4741 ggagcgaaac cactttgact atcgggcaac atattttcgt cagattccag ttttatgcca
     4801 cgaatactaa cactgtcgaa aacatctgat gatgaccatg tatctcctag cgttaattca
     4861 cttctgaaag gaataataac tcgctgcaac gtactactga tatgctccag tttacctgct
     4921 gaattactcc cacgactcca ggttgaataa tcgcgaaaac gccatgcacc aatattgatc
     4981 ccagcgcgaa gatttaaata atagttattt gaagagtaat caccacgatc gttattacca
     5041 gaaaagtcat aattcagcaa aaaagctgta ataccgttat cccactgttc tggtggaata
     5101 tatccgcgag cgtcacgttt tattatcgcc tgtggaaagg tcagtttaag tctctgtgca
     5161 tccagctccg gcatgtaatg cacatcggga aaccagatac gcaaatcaag gcaactattt
     5221 tcagatgaag ccaaagcctg ctctggcagc gcatttttgt taattccaag gcttaataag
     5281 tcagcaagtg aaaggcaggg aatcagttga ttctcatcgt ctgctttaaa cgttacagta
     5341 cgagagagta aaggggaatc attgacccaa acttcgactt catagctacc aggaagataa
     5401 gtcatccctt tctcgaagcg ggataagtca gcaacagctt cagtatcacc cgacagaaaa
     5461 gcagggttaa agcgcattcc cgcgattgaa catgcaggca taataaccag aagaagcggc
     5521 acaacccgtt tcatctgctt ttgtgccgcc atccaatatt cgcatctttt gagctttagg
     5581 ttcatatccc tgacctgtgt tgcatctgaa ctattccgtt gttactgcat tattcctttc
     5641 tgccgctccg tcgtagcgcc aaagtcatta attgtttgat aagtaatttc accagccaac
     5701 gtcactggcg acgagaccgt aatatcactt ttcggtggta caaacatatt gggcaattta
     5761 ctcccttgta ctttaattcc tgtcagtgtc aggtaataag gcgaaggatt gataactttt
     5821 agttgtttgc cctcatactt aaaccgcaac atatttcgtg cttttcctgg ttgcacaggt
     5881 agattttccg ggcgataaaa tagcttgatt ttgttttgta atgccaactg taaggtattc
     5941 ttctgtgtat ttttctcatc tgttggcgga attgctttaa cattcatcca atacaaagtt
     6001 tcacgatcgg tcggcaaatt agtcttacca agaaacatga tacgtaatga agcttcttta
     6061 cgcgttggca acataaagag gggaggagtg ataacaaaat cctggctctt tttttccgtt
     6121 ttactgtcat caacccatga ttgaattaaa taactggtgt tgtcaccgct attttgcaca
     6181 ctgagtgtta tttgcttact ttctgccgga taaatcaatc gcgttacgcc aaggccaaac
     6241 ccgcccgcgg acgcattatt aataaataat atcgat
//
LOCUS       DAZKHK010000202         6272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKHK010000202 DAZKHK010000000
VERSION     DAZKHK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.203"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(414..1103)
                     /gene="yjcO"
                     /locus_tag="ACHOS5_004751"
     CDS             complement(414..1103)
                     /gene="yjcO"
                     /locus_tag="ACHOS5_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP9779443.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(1214..1918)
                     /locus_tag="ACHOS5_004752"
     CDS             complement(1214..1918)
                     /locus_tag="ACHOS5_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9779444.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(1905..2741)
                     /locus_tag="ACHOS5_004753"
     CDS             complement(1905..2741)
                     /locus_tag="ACHOS5_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9779445.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(2734..3567)
                     /locus_tag="ACHOS5_004754"
     CDS             complement(2734..3567)
                     /locus_tag="ACHOS5_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9779446.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(3567..4616)
                     /locus_tag="ACHOS5_004755"
     CDS             complement(3567..4616)
                     /locus_tag="ACHOS5_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9779447.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSVFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(4716..>6272)
                     /locus_tag="ACHOS5_004756"
     CDS             complement(4716..>6272)
                     /locus_tag="ACHOS5_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9779448.1"
                     /translation="LLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEANELS
                     SIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGNPVRP
                     EDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPALALN
                     ILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEANASS
                     PTGAPKLKNVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMSIASA
                     EQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVGFPGA
                     LEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVKVDLL
                     PAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWKIPEL
                     NKETLAAIAEPDSAKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQGLNA
                     DMVWFDQVTK"
BASE COUNT         1534 a         1629 c         1622 g         1487 t
ORIGIN      
        1 tttttgtagg tcggataagg cgtttacgcc gcatccgaca tcaacgcctg atgcgacgct
       61 taacgcgtct tatcaggcct acgccagaca gcgcaatagc ctgatgtagc gtgattttgt
      121 aggtcggata aggcgtttac gccgcatccg acatcaacgc ctgatgcgac ggttgccgcg
      181 tcttatcagg cctacgccag acagcgcaat agcctgatgt agcgtgattt tgtaggtcgg
      241 ataaggtgtt tacgccgcat ccggcatcaa tgtctgatgc gacggttgcc gcgtcttatc
      301 aggcctacgc cagacagcgc aatagcctga tttggcgtga tttttgtagg tcggataagg
      361 cgtttacgcc gcatccgaca tcaatgcctg atgcgacgtt tgctgcgcct accttaaccg
      421 ttagttaatt tttcaaactc ttcacaaccg gtatcaaatc cttccataca gctcagattc
      481 aaccagtgca gcgccttttg tttattcttc tcaataaaac cctcttcacc gtttaagaac
      541 atcattcccg cccagtattc ggaataaccg gttcgggaaa tcgcagagct gcgtttgaaa
      601 taccaggttg ctttgtcatc gtcggcctta atgcccacgc cgttggcgta aatcaaacca
      661 agcagcattt gggcatcgac ggcagagtcg ttctccagat cttccgaggc gttttctaac
      721 agcgaaatcg cctttggata atccggttta cccgcttgcg tatttaccag aatatgcgcc
      781 agcgttactt caccttcttt actgcccgct tgcgctgctt tctcggcaag cacttttgct
      841 tgtggatagt ccagactgac cggattggtg attttgatct gcgccaacag cgcgcaggca
      901 tcggcatcac cgctgtcggc ggctttctgt gcccaatatt cggctttgct caaatcgccg
      961 gagctaaacc agctgtcggc aagaaaatat tgtgcgcgtc ggtctccggc ctctgctgcc
     1021 tttaaatact ggctgccagg ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac
     1081 atcaaagtga taattttttt cattttgata atgaagtttg gtaggggaga gacagtataa
     1141 agtgttcggg tggacaaaaa aacaactgta aaaagacctg aaagcagaat gaattaatgc
     1201 aatgtcaggt tttttaatcc atccgatgtt cccccttttc catggcgtcg cgatcaaaaa
     1261 atcgctgaat ttttccctcg gacatcaatg ccgctcgatc agacatatgc gcgatgacat
     1321 cggcatcgtg actgaccagc agatatgtca tttggtgttc cagcttaagt tgattcaaca
     1381 aattaaggat ttccgcttgt accgacatat ccaacgccga ggtgggttca tccagcagaa
     1441 gaagttgtgg tcgtaacaac agggcgcgag caataaccac acgttgccgt tgaccaccgg
     1501 aaagctggtg tgggtagcga cgagcggcgt cgaaaggcaa gccaacctgt tccagtgcag
     1561 tctggacgcg tttttctatt tctctttcgc cacgaatttt gagtggttca gccagcgttc
     1621 gccacagagt atgattggga tgcaacgagg catagggatc ctgaaacacc atttgcacat
     1681 tgcggcggag atcgccctga aaacgcattc ctggtttgat ggtgtgccca aacagtgaca
     1741 cctgaccgtg ccagtcgcgc tgtaatccag caattacgcg caaaatcgtg gattttccgc
     1801 agcctgattc gccaattaaa ctaaatgtct ctccggcatt aaccgaaaaa ctggcggcag
     1861 aaactgcggt tttctcagca aaagtcacct gtaagtgatt aatttcaaca atcgtcatgg
     1921 tatttctccg gcgtcattgc cgtcctgtcc agcgtcggca gcatctgccc gtaagtttgt
     1981 gcatttgggc ggcaagtcca cagtgtgcgc gtgtaaggat gcgttgctgt aggtagcgca
     2041 gcagcgtgca tttcatccac cttatttccc tgatacatca ccagcacccg atggcaaaat
     2101 tgggcaacta atggcaggtc gtgactgatc agcaacattg ccatctgacg ttgttcacac
     2161 tgggtaacca gcagttccag aatctgatta cgcaggcgca catccagtgc tgatgttggc
     2221 tcatcggcaa tcaatacccg gggatcgttg atgagcgcaa ttgcaatcat cactcgctgc
     2281 cccatgccgc cagaaagttc acgaggatag ctttgcagta ctcgcgtatt gaggccgaca
     2341 gcctgaattg cggtgtttat tttctcttct ttctcgcggc ggttcagacg ttgatggagc
     2401 gtaagcgcct cttccagttg cgcttttact gtctttactg gattaagcgc gtaacgcgga
     2461 tcttgcaata ccatcgcgat atcgttaccg cgcaactgtt gccatccacg cgcgttaagg
     2521 gtgagtgcat cacgccccag aatgttaagt gtgtccgcgg ttactacacc cggtttacgc
     2581 accagcccca tcaaggcgcg ggcggtcatg gacttacctg aacccgactc accgaccagc
     2641 gccagacgtt cattgcctaa tgtaaaactg acattgttga cgacccggga ctccggataa
     2701 tcgacgcaga gattgttgat actaagacgt gagtcagtca tgttgaggct ccagaacatc
     2761 gcgcaagcca tcacccagca gattaaaggc cagactggca attaagataa ttgcgccggg
     2821 agcggcggca atccaccatt gatcaaaaat gacctgcata ccgtcggcga tcatggctcc
     2881 ccattccgcc attggcgggc gagcaccaag cccaagaaaa cccaggccag cagccgccag
     2941 aatgataccc gctaaatcca gcgccagacg gacaatggca gatggcaggc acaatggtaa
     3001 aatatgacca gccagcagac gcctgccgcg gatgcccatc atttctgcgg cggcaagata
     3061 atcactgtgt cgcagacgtt ggatctcact ccgtgcctga cgcgcataag caggccaggt
     3121 ggttaatgcc agggccagtg cgccgttaac cagccccggc cccagcatgg caacaaaagc
     3181 aaaagccaga attaaacgcg gcattgacat tactacgtca gtaaaacgca tcagcacgcg
     3241 ttccagccag ccgccgtagt acccggctaa aatgccaatc agcagaccga ccgggagtgt
     3301 aatgattgtc accaatgcca ccagccccag tgccgggcgg ctgccgtaga ttaaacgtga
     3361 aagtaggtct cgcccgtaac tgtcagtgcc gagccagtgc tgacgactcg gtgcttgtaa
     3421 ccgcgccgcc gcatcctgcc agttaggatc gtgaggtgcc agccacggcg cgaagagcgc
     3481 aattagcatc aataacagga taattatcaa cccgcaaaac gcggcgggtg aatggcgtaa
     3541 acggcgtaaa aagagaaaca tcggcatcag cgtaacctcg gatcggtcag ccggaccagc
     3601 aggtcggtta agttatttat caacacaaag cagacgccaa tcagcagcgt tcctcccatg
     3661 attgctgtgg tatcacccgc gaagagcgct gtagtgaggt agcgcccaat acctggccat
     3721 gaaaagacgg tttctgtcag taccgcgcct tccagcattg atgtataggc gagggcgata
     3781 acggtgagca aggtgccgcg aatgttgggt aaaacatgac gtagcaaaat tgtcatctcg
     3841 ctggcccctt tggcgcgcgc cagcagaata tattctttat tcatttcgcc aagacaggcg
     3901 gaacgagtca ggcgcgtaat actggcgagc gagaagtacg ccagtaataa aaccggaagt
     3961 atcaaatggt tgattgcatt ggtaaatgcc tcgcgatcgc cagaaagcca cgtatcaatc
     4021 agcgcaaatc cgctgcgggg ttctatggtg aattgataaa tatcatccag cctgcccggc
     4081 ccggcactcc attgcagtct ggcgtagaac agcgccagca tcagcagacc aagccaaaaa
     4141 acagggacgg aatttcccag tagagttaat gttcgcacgg tgaaatccag cggagaaccg
     4201 acataacggg cgcaaagtac acccgcaatg acgccgacaa cagcaccgac aataagcgcc
     4261 aacgttgcca gttcaatggt cgccgggaat actgatagca agtcctggag taccggttgc
     4321 ccggtggagc tggcgatgcc cagatcgccg tgtgccagat tcatcaggta atgccagaac
     4381 tggacggcta acggctgatc caatcctaac tggtggcgta cctgatcata ggtggactgg
     4441 ctggcgtgat cgccaacgat ttgtagtacg cgatcgaccg gtgagagagc cgaaagcgca
     4501 aaggtaatca gcagcaggcc gagtagcgtc agagcaacgg taaacagccc ttgcaacagc
     4561 gcgagcagag ggcgcttgcc tcgccatctc gcgcgagtgg aaaaaacgac agacatgatc
     4621 tttccataca gtgataaatc agcggctggc ctgcaataca ggccagccct ggtaccgcgc
     4681 ccggtaacag ggctggacgc gagttgcata cagggttatt tggttacctg atcaaaccag
     4741 accatatcgg cattcaaccc ttgttgatac ccctgcacat tatcacgcat gacaatttgc
     4801 gttttgcctt gatcaataaa gacatacggg gaactacgtt gtagttcttg ttgcattttc
     4861 ttatacaaat caagacgttt agcgctatca ggttcagcga tggccgccag cgtttctttg
     4921 ttcagttccg ggattttcca gccgtttaac ccggcaacgg tgctggattt gccatcgtta
     4981 taggcgaaag agctggcgtt ggaatgtgcg tcaaaataat caggcaacca catacgaatg
     5041 gccccctgat gctggtgggc gcgaacgcga gagtagacct gactccctgc ggctggcagc
     5101 aaatcaactt ttacaccgcc ctgagcaaag ctggcctgaa ttgactgcgc gatggtaata
     5161 aatggcggtt tattttcgac atcaagcgtg aagtgcgggt ctttgattcc ggctttagcg
     5221 aggatctctt ttgctttggc tggattaaag gtaaacggtg tttcttccag tgctccagga
     5281 aagcccacgg gcaggaaact ttgatgggta aaatattgtc cttttaagag atttttggtg
     5341 atgccgtcgt aatcgaccaa ccagcgagcg gcctcccaga acgcaggatt actcatcagc
     5401 gggttatcct tgttaccggt attgaaggcc aggtagttct gttcggcgga ggcaatgctc
     5461 atcactttta cgccaggttt accctgtaat gcatcgattt gatcggctcc cagatcacga
     5521 gccatatccg catcaccttg ttggatcagc aggcgacgcg ttgccggatc gggaacattt
     5581 ttgatgatga cgtttttcag tttcggggcg ccggttggtg agctggcgtt ggcttccatc
     5641 acgatggcct gatgcggttg gtaagtacgc attttaaacg cgccgctgcc agcggaatgc
     5701 atcttcagcc attcattacc aaagtcgtta tttttggcgt ttggcgcaac cagcttctcg
     5761 tcaacaattg aggcgattgg tgtggagaga atattcagtg ccagcgccgg gctgacgtcg
     5821 gcactccatt gtacctggac ggtatggtca tcgattttct tcaactggct ggcgatattt
     5881 tccggctgcc atcccagcac attgagaata aacgcaccgg atttattcag tgtgacagcc
     5941 cggacataag agtaaatcac atcttccggg cgtaccggat taccagaggc aaatttcgca
     6001 tcgcttttca gttttatggt tagcgttttg gctgcggggt cagcctgcca actttccgcc
     6061 agaatcggag tgattttcgt cggatcatta cggtccggtt gtactacgcg ttgatacaga
     6121 cttggtacag tctgaatact tgagagttca ttagcttctg ccgggtccag actcacaata
     6181 tcatccagtc cctgggctac aaccaacgta ttcggcggtg tagcggcaac ggttgtggcg
     6241 gaaattgtgc ttaaaaccag taaaggtaat ag
//
LOCUS       DAZKHK010000203         6247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKHK010000203 DAZKHK010000000
VERSION     DAZKHK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.204"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            81..332
                     /locus_tag="ACHOS5_004757"
     CDS             81..332
                     /locus_tag="ACHOS5_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9779449.1"
                     /translation="MFSLIQRGKRYVDAAGYPVKICFCSADKVVYRRLDNQTRSVCIA
                     EFNQNFERLDYHEYIRIKAEIEQTEHLQELRCLYKRGGK"
     gene            772..1002
                     /locus_tag="ACHOS5_004758"
     CDS             772..1002
                     /locus_tag="ACHOS5_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9779450.1"
                     /translation="MANRKQHRAIAERRHIQTEINRRLYRALTIARVMYFNMLCDQGC
                     RIAPDYIASTFSYLADDLRDMQRLVNEPGNNP"
     gene            1081..3003
                     /locus_tag="ACHOS5_004759"
     CDS             1081..3003
                     /locus_tag="ACHOS5_004759"
                     /inference="COORDINATES: protein motif:HMM:NF017640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9779451.1"
                     /translation="MTVYSAGGPRMPEPEEELIFTEEVNSAFSGLPRFVSVKLGRRIS
                     SAWQTKGFAGAREKFALMVQKDLPCITGVNKRYAINPDELPGWLFGGLASDQAYGAVH
                     SMTWRFNSLVDGDDGDAHLLAQDLSAFLIAEMQHLSALLADEDADVAAGAIYAMVAGL
                     VEHFRMEPPDWKRFIGRKMTLDQLTVAISRMLSVRWWQGKLRVFSRRWREHLLIAMGS
                     VRRQSSPHCSYEWVQRWLAGRKHGRRLMAETDVEDEETGEKFSLLDLVDSSISDAEKR
                     RSELMTRVKGLEDLARFEGVAQEQGYVGLFFTMTAPSKYHAWLETGYRNRRYNGASPK
                     DTQNYFSGLWRQISATLRRRGIGVFGLRSAEPHHDGTPHWHGVLYVHQEYAVALQEIL
                     EYYANRDDDAEKVTIRKAGRKSHLEIKPVLDDLGSATAYIAKHIGMNIDGCASGGINH
                     ETGHTLVVTARHASAWASLWGIKQFQFIGSAPVSVWRELRRFNNQAHADRISPALGEL
                     HRAASEGDWKTYTRLQGGPFVARSDLVLRTWYQRSEEPDECGRHASAIKGVYMPGGDT
                     PPVVTRSRKWRISRNVTTGSLGVNRGPALTPWTCVNNCISGRKRPADHPPDLCIRIPV
                     QLELNFWEEKKGKNEH"
     gene            3117..3398
                     /locus_tag="ACHOS5_004760"
     CDS             3117..3398
                     /locus_tag="ACHOS5_004760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779452.1"
                     /translation="MRWIMRGLELERMTPEKMHAIGMECIRRSEAMAQAGMTKDAVRK
                     NLLPAVQRLNRAKHELQLVVDQMIDKAAELEKYGSALEKIVQDIVGTEQ"
     gene            3409..4488
                     /locus_tag="ACHOS5_004761"
     CDS             3409..4488
                     /locus_tag="ACHOS5_004761"
                     /inference="COORDINATES: protein motif:HMM:NF017914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9779453.1"
                     /translation="MQAILTVFPQRNAGITLIKTGNLTARFRDGQRVMIRDVPAVLAG
                     VPSGEIPSSGQGLAGEEELKDFFADDRVIKRAGGSDALARWVSRIAGCQCENGDHVEN
                     MMTARTMQGGAIRLCHACDNRHYMKGYRELADVIARNRAEWLMDFIRMNLGLGEDHQV
                     SIPELFCWAVLNDVADAVPVEIVRRIRRLPEEEQLRGTMREADINPWQISADAVVKKL
                     TERAKPVSTHNALVAHAVRREKTDEQERAVLKLATDSDPVMGYMRRPRMQRLVLPDYT
                     AWVKRQRCCGCGKVADDPHHITGHGFGGAGIKAHDLFVIPLCRECHQALHNDRKGWEE
                     RNGSQLLWVLRTLDRAAGIGALAGG"
     gene            4490..5158
                     /locus_tag="ACHOS5_004762"
     CDS             4490..5158
                     /locus_tag="ACHOS5_004762"
                     /inference="COORDINATES: protein motif:HMM:NF018074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9779454.1"
                     /translation="MTPQQMVWAREQVIFALNPPDIRHKGQLEPFGDFARSDTKRYGR
                     QRKRTLNIDGRWYCRDSDPVFAAETPRYSAQSEMITPEAFRTAKLRRKIYCLEDYQRA
                     WVLFSYSLQKKRIHHLLVSEFIWMRVRERLRGKRVTERMIGNLIRLTRIVALNAAVIA
                     GGVVAGAVLIAPSCAAQQMDIKPNTWSQHYKGYWCFMHEVCYELDHEALITIMGIKKI
                     TRKI"
     gene            complement(5262..5660)
                     /locus_tag="ACHOS5_004763"
     CDS             complement(5262..5660)
                     /locus_tag="ACHOS5_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9779455.1"
                     /translation="MLYPVAIDKSDSSFGVRVPDIPGCFSGGNNYQDAIESAHEAIEA
                     HIELLVENGEAVPKATCIENWLADPDYSDAVWALVDVDITRLMGKAEKINVTLPSLLI
                     RRIDQFVAAHPEYGSRSGFLSRVAADKITG"
     gene            complement(5677..5856)
                     /locus_tag="ACHOS5_004764"
     CDS             complement(5677..5856)
                     /locus_tag="ACHOS5_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9779456.1"
                     /translation="MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKD
                     IPLPTLKSIKKQAGL"
BASE COUNT         1547 a         1306 c         1852 g         1542 t
ORIGIN      
        1 actgaagtgt tctcaaatgt tggtattgcg ttggcgattg tgctggttgt gtactcaatt
       61 tgccgctggg ggataaatac atgttctctc tgatccaacg tggtaagcgg tatgttgatg
      121 ccgctggtta tccggtgaaa atttgttttt gttctgctga taaagtggtg tatcgccgcc
      181 tggataatca gacacgttct gtctgtatcg cagaatttaa tcagaatttt gaacggctgg
      241 attatcacga atatatacgg attaaagcag agattgagca gactgaacat ctgcaggaat
      301 tgagatgttt atacaaaaga ggtgggaaat aaatacggat acagaaattt aaccttcgcc
      361 tgaattaacc ggaaataaga ggaatgagaa atacaccgct catgcggcgg gattcctgca
      421 accttaaaac gggataagag aaatagcaga agtgaagcca gtgacgatac aaaaaaaccg
      481 cttgccagtg ttcacctggt cgggttacat ttccgttgca cctcataaaa cgggggccgg
      541 gattctcaac ccgctgacta tgcacgcgca caaccgcgcc agcggttttt ttgtgcgtac
      601 cgtatcgcca catctttttc tcgcaagaat catggtgggg cgtgtggggc cgactttggt
      661 cgggccggag tcgtgcgtag ccggtgttga gaaccccata cgtcctgcca ccccgagatt
      721 ctcaactcgc ggctgtgggt tatcacaact cactacgtac gaggccacac catggcaaac
      781 cgtaaacagc accgcgctat cgcggagcgt cgtcacatcc agaccgaaat caaccgcaga
      841 ttgtaccggg ctctcaccat cgccagagtt atgtacttca atatgctttg tgatcaggga
      901 tgccggattg cacctgatta cattgcctcc acgttcagtt acctggcgga cgatctgcgg
      961 gatatgcagc gcctggttaa cgaaccgggg aataacccgt aaagctcttt ttcctgaaat
     1021 cctgaaacgg cactccgcca tctggcgggg tgtctggtta cccgttatgc gggaggacat
     1081 atgaccgtgt attcagcggg aggtccgcgc atgcctgagc cggaagaaga gttgatattc
     1141 actgaggagg tgaacagcgc tttttccgga ctgccacgtt ttgtgtctgt aaagctggga
     1201 cgccggatca gcagcgcgtg gcagacaaag gggttcgccg gtgcccggga aaagtttgcg
     1261 ttgatggttc agaaggattt gccgtgtatt actggtgtga ataaacgtta tgccattaat
     1321 ccggatgaat taccgggctg gctgtttggt ggccttgcgt ctgatcaggc atacggcgcg
     1381 gttcattcca tgacatggcg attcaattcc ctggtggatg gagacgacgg ggatgcacat
     1441 ttactggcgc aggatctgtc cgcgtttctt attgctgaaa tgcagcatct gagtgctctt
     1501 cttgcggatg aagatgcgga tgtggcggca ggtgccattt atgccatggt tgcgggtctt
     1561 gttgaacatt tccgtatgga gccaccggac tggaaacgtt ttattggcag aaagatgacg
     1621 ctggatcaac tgactgttgc catcagtcgg atgctgagtg ttcgctggtg gcagggaaaa
     1681 cttcgggtat tttctcgacg ctggcgtgaa catctgctta tagcgatggg gagcgttcgt
     1741 cggcagtcat caccgcactg tagttatgag tgggtgcagc gttggttggc gggacgtaag
     1801 catggtcgca ggctgatggc agaaacagac gttgaggatg aagaaaccgg ggagaaattt
     1861 tcacttctcg atttggttga cagcagcatt tcagatgcgg agaaacgtcg gtcggagttg
     1921 atgacgcgtg taaaggggct ggaagacctg gcacgctttg aaggtgtggc gcaggagcag
     1981 gggtatgtcg ggctgttttt cacgatgacg gcaccatcga aatatcacgc ctggctggag
     2041 acgggatacc gcaaccgtcg ttataacggc gcaagcccta aagacactca gaattatttt
     2101 tccgggctat ggcgtcagat cagtgcaacg ctgaggcgcc gcggaattgg ggtttttggt
     2161 ctgaggagcg ccgaaccgca tcatgacggt acgccgcact ggcatggtgt tctttatgtg
     2221 catcaggagt acgccgtcgc cttacaggaa attcttgagt attacgctaa ccgcgatgat
     2281 gacgcggaaa aagtgaccat ccgcaaagca ggaagaaaat cccatctgga gataaaaccg
     2341 gttcttgatg atctgggaag tgccactgcc tatatcgcca aacatatcgg aatgaatatt
     2401 gacgggtgtg cgtccggcgg aattaaccat gagacgggcc atacgctggt ggtgacagcc
     2461 cgccatgctt cggcatgggc atctttgtgg ggaataaagc agtttcagtt tatcggcagt
     2521 gctccggtga gtgtgtggcg ggaactgcgt cgttttaaca atcaggcgca tgctgacagg
     2581 ataagtccgg cgctgggaga attgcaccgg gctgcgagtg aaggtgactg gaaaacctat
     2641 acccgtctgc agggtggccc gtttgttgcc cgaagtgatc tggttcttcg cacatggtat
     2701 cagcgttcgg aagagccgga tgagtgcgga cgtcacgcca gtgcgatcaa aggtgtttat
     2761 atgccggggg gtgatacacc accggtagtg acgcgttcac gtaagtggag aatcagccgg
     2821 aacgttacaa caggctctct cggtgttaac agagggcctg ctttaacgcc ctggacttgt
     2881 gtcaataact gcatttcggg cagaaaacga cctgccgatc atcctccgga tctttgtata
     2941 aggatccctg ttcagctgga gctgaatttc tgggaggaga aaaaaggaaa aaatgagcac
     3001 taaattttcc ggttaaggca gtggagaaag cggggtgtcc ggagaagaaa aatacctgtt
     3061 ttacctgagt atgtttttat atacagtgtc gtcatatggc gacaattgag tgaaagatga
     3121 ggtggatcat gagagggctg gagcttgaaa gaatgacgcc ggaaaaaatg cacgcaattg
     3181 gtatggagtg catcaggaga tctgaggcta tggcgcaggc ggggatgacg aaagacgctg
     3241 tcagaaaaaa cctgcttccg gcggtgcagc gtctgaaccg tgcgaaacat gaactgcaac
     3301 tggtggttga tcagatgatc gataaggcgg cagagctgga aaagtatgga agcgcacttg
     3361 aaaaaattgt tcaggatatt gtgggtactg aacagtaaga ggtgtgatat gcaggcgatc
     3421 ctgacggtat tcccgcagcg taatgccggg ataacactta taaaaacagg caacctgaca
     3481 gcacggttca gggacgggca gagagtaatg ataagggatg tccctgctgt gctggcaggg
     3541 gtgccatcag gagaaattcc gtcatcagga cagggactgg cgggagaaga ggaactgaag
     3601 gatttttttg cagatgacag ggtgataaag cgtgctggtg gcagtgatgc cctggcacgt
     3661 tgggtgagca ggattgcggg atgtcagtgt gagaatggcg atcatgtgga gaacatgatg
     3721 acagcgcgga caatgcaggg aggcgctatc cggttatgcc atgcctgtga taatcgacat
     3781 tatatgaagg gataccggga gctggcagat gtgattgcca gaaatcgtgc ggaatggctg
     3841 atggatttta tcaggatgaa tctgggactg ggggaagatc atcaggtatc cattcctgag
     3901 ttgttttgct gggctgtgct gaatgatgtg gcggatgctg ttccggttga gatcgtaagg
     3961 cgtatcagga gactgccgga ggaggaacaa ctgaggggaa cgatgaggga ggcggatatt
     4021 aacccctggc agatatctgc tgacgcagtg gtgaagaaac tgacagagag ggcgaagccc
     4081 gtaagtacgc acaatgctct ggtcgctcat gcagtacgtc gggaaaaaac ggatgagcag
     4141 gaaagagcag tactgaaact ggcaactgac agtgacccgg ttatgggcta tatgagaagg
     4201 ccgaggatgc agcgtttggt tctgccggat tatacagcat gggtgaaacg gcagcggtgc
     4261 tgtgggtgcg gaaaggtggc agatgatccg catcacatca cagggcatgg atttggcggt
     4321 gcagggataa aagcccatga cctgtttgtg ataccgcttt gccgggaatg tcatcaggca
     4381 ttgcacaacg acagaaaggg atgggaggag aggaacggca gccagctact gtgggtgtta
     4441 cggacactgg acagagctgc gggtattggc gctttggctg gagggtaata tgacaccaca
     4501 gcagatggta tgggcaagag aacaggttat ttttgcgctg aatccaccgg atatacgtca
     4561 taaagggcaa ctggagccat ttggtgattt tgcccggtca gacacaaaac gttacgggag
     4621 gcagagaaaa agaacactga atattgatgg tcgctggtat tgccgggaca gcgatccggt
     4681 atttgcggct gaaacccccc gctactctgc gcagtcagag atgatcaccc cggaagcatt
     4741 cagaacagct aaattgagga gaaagattta ttgcctggag gattatcagc gggcgtgggt
     4801 gttattcagt tacagtttgc agaaaaaacg aatacatcat cttctggtca gtgagttcat
     4861 ctggatgcgg gtgcgtgagc ggttgcgggg aaaacgtgtt acggaaagga tgatcggaaa
     4921 tcttatcagg ctgacaagga tagtagcgtt gaatgctgcc gtaattgctg gtggggttgt
     4981 ggcaggtgca gttctgattg cgccttcatg tgcggcgcag cagatggata taaaaccaaa
     5041 cacatggagt cagcattaca aggggtactg gtgttttatg catgaagtgt gttatgaact
     5101 ggatcatgag gcactgataa ccattatggg aataaaaaaa atcaccagaa aaatataaat
     5161 ttgctaaagt gaatattttg aggtaaattt tagtcaaatt tgatagtctg ccagagttgt
     5221 aagtagagta aaaacccgcc attgagcggg tttttctgca ttcatcctgt gattttatct
     5281 gcagccacgc gggacagaaa accggagcga ctgccgtatt cggggtgcgc cgcaacaaac
     5341 tgatcgatgc ggcggattag cagagagggg agtgtgacgt tgattttttc cgctttgccc
     5401 atcaggcgtg tgatatccac atcgaccagt gcccacacgg catctgaata atctggatct
     5461 gccagccagt tttctataca agtggctttt ggtacggctt cgccgttttc aaccagtaat
     5521 tcaatatgtg cttctatcgc ttcatgtgcg ctttcgattg catcctgata gttgttgcca
     5581 ccagagaagc agccgggaat gtcggggacg cgaacgccga aagatgaatc gcttttatca
     5641 atagcaacgg gatataacat acaaacctcc tggggcttaa agccccgctt gttttttgat
     5701 gctttttaat gttggtaacg ggatatcttt ctgtggatgt ttcactgtta ccagcccttt
     5761 tttcgtcggg tgtttgaact ggtgatgact gccttttacg cgcaccaggt accacccgtc
     5821 agcttcgatc atcgctattg catttctgct gtccatcctc cgactctctg tgttttctta
     5881 ttggggttat tataactcca atatgtttac ggtcaagtat tttggagtta ttggggttat
     5941 tgctttcgtg tggtgagagc aagcgactga taattgccag gtcactggtt cgaatccagt
     6001 acgacgcgcc atatttattt accaggctcg cttttgcggg ccttttttgt ctccgcgcca
     6061 cgcccggcgc atatcaacca cagagccttt cacaaatggt ttttcaggag tccaataatc
     6121 gcgaagtcgc cagaaaatac gcagagtaca tcacgtccgt tcctttgcgt cggtttgtcg
     6181 ctgaaaaagt gcgggaatat tgtggtgatc tgcccctgag tatttttgat ggtgcgtgtg
     6241 gctccgg
//
LOCUS       DAZKHK010000204         6222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKHK010000204 DAZKHK010000000
VERSION     DAZKHK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.205"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..637)
                     /locus_tag="ACHOS5_004765"
     CDS             complement(104..637)
                     /locus_tag="ACHOS5_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779457.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVI
                     LEDKLAEKPVTGKNRKN"
     gene            complement(640..1566)
                     /locus_tag="ACHOS5_004766"
     CDS             complement(640..1566)
                     /locus_tag="ACHOS5_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFP9779458.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTPTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQDRTEPERVKSPSRGFSMGF"
     gene            1744..2142
                     /locus_tag="ACHOS5_004767"
     CDS             1744..2142
                     /locus_tag="ACHOS5_004767"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779459.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKEIKYDLETSPNPDA"
     gene            complement(2403..3422)
                     /locus_tag="ACHOS5_004768"
     CDS             complement(2403..3422)
                     /locus_tag="ACHOS5_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9779460.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSD
                     KGKKSAAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKK
                     QIIKC"
     gene            complement(3919..4062)
                     /locus_tag="ACHOS5_004769"
     CDS             complement(3919..4062)
                     /locus_tag="ACHOS5_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP9779461.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            complement(4124..4384)
                     /locus_tag="ACHOS5_004770"
     CDS             complement(4124..4384)
                     /locus_tag="ACHOS5_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP9779462.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLT
                     EHPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
     gene            complement(4387..6114)
                     /locus_tag="ACHOS5_004771"
     CDS             complement(4387..6114)
                     /locus_tag="ACHOS5_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFP9779463.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     IYVSVTDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPG
                     KATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPEL
                     SKQFKDSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPK
                     RHIDIHRGK"
BASE COUNT         1505 a         1719 c         1340 g         1658 t
ORIGIN      
        1 gggagggata ttttttggca gttcggagga atcactcatg aaaacggtga acggaacgcg
       61 aaatcctttc gctgtccgtg aggcgttgga caagcgaagc gcgtcagttt ttccggttct
      121 tacccgtgac aggtttttcc gccagtttat cttccagtat cacaactttc tgatacaccg
      181 ccgctgtggc gttgtagttc ctgtcgagca ttgttttcat tccccatccg agcacacaga
      241 acataactag aaaagccact gacaccccac ccagccattt ccagctatac cctttaaggg
      301 tgttgtattc ctgcacgata ttgctgtgaa ttgtcgcaat atccctgttc agggcatccg
      361 cgtttgcctt catgtttgcg actgactctt ccagccgctg aaaatcctgg ctgatgcgct
      421 cacctatcag ccctgctacg tccagattct gcacctcatt tctgacttca tccctgacct
      481 gttttgccac accgtcagaa atatcccgat atccgcgaag tgtttccctt gccacagcca
      541 gagaatcagc aacagctttc tgttgctcca ttgcggtatg ttgcaaatgc tccaccgata
      601 acaggagcgt gagtaatttt tcttctgtgc tggccatgtt cagaatccca tagaaaatcc
      661 acgtgacggt gatttaaccc gttcaggttc agttcggtcc tgtctgctca ctctgtctct
      721 catgtcaaag ccatgctccc gcgcaacatc atgttgcagc cttctgacca gttcatgttg
      781 cttaaccgga gtgtccagcc attccatgat gtcatgcccg accgttcgcg cctgttcaag
      841 atggcgggaa tagtccggtt cagaccgttt ttgcatgatg tcatgacgct gggcatcatc
      901 aagccgtccg gcagcttcaa aacccagatg cacttcggga cgtcgttcaa tgccctgttc
      961 tttcagcgtc cgacagtcaa tgaagcccct ttcaaagcca ttttttctca tatgttcgtt
     1021 gtgcttcacc tcccagcgtt ttcgcaattc ccgcaactgc tctttttgct gagtgggagt
     1081 caggcttcct gaatcctttt tcgcacctcc ccgctcggga tacctggcgt tatagcgccg
     1141 gaaatactgt tccggtgtac gttcaatgcc atcattcaca cgctgactca tcatgatatg
     1201 cgcatgaggc tgttcgcctc cctcaatgct cgctttcgga ttgtgaatgg caaaactcca
     1261 tgcatgccgt tcgccagctt cctgacgtac aaaatcctgc accagttcaa ggcgctgttc
     1321 cggtgtcagt tcacggggca gcgctatctc cagttcccga taagtcgaac cgtttgcacg
     1381 ttcaaactgg tcagccgcct gccagaaatg tgagggatta tctcgcgccc attccggcat
     1441 gtttccgtgt gcggaatatt ccaaatcctg acggtcgcgg tatttctctt tgcgctcgat
     1501 gtaatcggcg tggtttgcag cctgcccctt gccaccaaat ttaacgctga ggtgataggt
     1561 tgccacggtt tcagccctcc gcaggagcgc actgtttctc gacgaaggag agaaacatat
     1621 aagtgcgctt ccttctaatt ttacgaatta gcacggaaga taccccctgc acccgccgac
     1681 cgcaagcgat atactgcacg accacaacac accgcaaaaa aaacaccagg gagcaccacg
     1741 aacatgcgca tcagcaacat cgaatggctg aagaaacgga tcggattcat caggaagctc
     1801 ggagaacaga ccgcacgaca gagacagatt attgacctgc tcgacaacga agcaggactc
     1861 acagaacagg aacgcaaact cctccacgta ctggcaacag cggaaaaaaa cgacttacag
     1921 gcacaggaga acgaacgaaa acaggcggtc caaaatcgaa tcgagggaaa aaaacaacgg
     1981 cgagaacgca atcaccgact ctttctcgcg gctgggctac tgatcgaagc aggactggta
     2041 gacacaaaaa caggcgaact atgctataaa aaagacagga tattacaggc acttaaggaa
     2101 attaagtacg atctggagac atccccaaac ccagacgcgt aagtgcctcc atccgtgttc
     2161 cggttccctg gtgcttcctc gctcattcgc tcgctacgct cggtcatgag actgcggcga
     2221 gcacgaccac ctcaatcatg cggtaagccg caccattgct gggctgagag gagaaaaccg
     2281 ccggaaagcc cgctgctcaa aaagacgccg aaaggcgctg ttatctgata taggcttgtt
     2341 catgtccgcg aatttaacag cccattaaca aaaaacgggg ggtagtgttg cttctttgtt
     2401 aactaacatt taattatttg tttcttggtt agccattctc cagaattatc catccagcgt
     2461 cgaatagtcc ggctgctaac accaaccgcc actgcaatat cttccgttga cgctcccaag
     2521 atatacatat caagcgccct acgacgtttg tcgtcgttag cagtccaccg aacagcggcg
     2581 ctttttttcc ctttgtccga ctgattttct gacgaattgg ccttcccgcc cttgcgaccg
     2641 cgtgctgcct gaatttctgg cgtgtgcgta tcagccacat actgtgcgaa agtttccggc
     2701 gtgaagttcc tgtgcgtgta tttcgcaatg ctcttgccaa tagcccgaca ttcagccagt
     2761 gaaagcggaa cgggaagcga tgcattgtac atttcgacac gctggatcac cgcttcaagc
     2821 cactgtgaga acgctggcca gccctgacga atcgccctgt aagcccattt gcgcgtcatt
     2881 tcgaacaggt ggcagtttcg ccccatcccg taatgtttat cgatgctacg acgctctgag
     2941 gcgctcaaat cgagataatc tgccagttca tcgagggtat aggcgtcctc gcgccactcc
     3001 atcacctgcc attccaggtg gaacggattt ttgcaaatca ggccgctgta gttcacatca
     3061 gcacaaagtt tctcacacag cgaacgttca accgctgcgg catatttcag cgccttaacc
     3121 gaggcatcag gcgcggttct cacggcgatg ttgagtgcgt agagcaggtg agcgtgtccg
     3181 ttcacaggat ttttaacggt gatgttcggc gcgggggcgt tccggtcgct ccagtcaatc
     3241 gcagccccct cgcggtcaac gtcaaaaacc agccagaact gcgcatgagg ctggttctgc
     3301 tggatgtacc gcgcaagaag cgcacgccct ttgccggaga tgcgaaggcc gaaagcgaga
     3361 tcatccgtgt gatagggacg atggggtaaa ttttcgtcga agtattgaag cgcggcgctc
     3421 atgtgttacg attctctccg ggttatgcga cctacacgcc ctctgtattt catgttgaat
     3481 cttgccggat ctggacatga tttcaggggc gtttgttttt cagccgctta cactacaccc
     3541 aacggcaaat cctgtcgatc cttcccgaaa aaccgatcca tttaccataa ctcccgttgt
     3601 acgcacccct taaaacgccc tcagtcgctt tctgacgcaa aaaacgcaaa aagacaagaa
     3661 aacaccaggc caaaccagaa aaacgcgcca gaatcgtttt tagagggctg cataactatg
     3721 cacgaaagcg atgcgcgatc gatttcgact gttccgaagg gcgaacaggt caaaatgaca
     3781 agttttctcg ccctgggtgg aaagttctgc acggggattt tagctctcat ctcccgcatg
     3841 gaatttttgt gacatttcaa aaaaaagcgc atcagaatca gcctgacacg ctttgttttt
     3901 gtcaaagagg atttcgggtt actgcgttgc taatccggtc acttcagctg ttgatgacgg
     3961 ggatacagtc ccgccctgaa catcccggat atagtttgcc tgacatgcag ccagaataat
     4021 gactgcaaga agcaataaaa taatccctgt tatttttttc ataacgactc cttgttgtga
     4081 aagaatgcca gccattcatc cataaacaac cggcattcat attttagccc tgtttaaatc
     4141 ctggcttacc gttagcagct cgccattctt taatttcttt aacaatccct tcaggactat
     4201 cttccctgtc atcgcgagga taataaatca gatctgaacc atctgggtgc tcagttaatc
     4261 gctcaaactc tctcactaat ttattgtcat cctcttcagt agcaccttca gctctacata
     4321 ttttttttac aaactccaga aattcagcct cggtataatc actaatacta tgtttcagtt
     4381 ccatttttac ttacctctat gaatatcaat atgtcgctta ggtgtggtca ctctgatatt
     4441 atccatatca tagacaccac catcctgact gattggttta tcatgatgta attcaaagcg
     4501 ttccctacca cctacttggt ctttcttcct tgcaaaaggt gcttttccct tttgaatgtt
     4561 cgtcttatta ctgtctttaa attgcttact aagctcggga tcttttgaca cttcttccca
     4621 gaatttcttc cggaaatcgt caaaactttt aaattcttta tcacgcaact tatcagcaat
     4681 gcgatcagga attggcgctc ctgaatcttt acccgcatca tcaagccatt tatcaccaac
     4741 tggttttcct ttacccgtcg ccttccctgg cttattccgt ttactctcct tatctaattt
     4801 atccttagag tccttttctt tattttcctt ctgcttacgg cgttccagcg cagcacctaa
     4861 cgcagcatca gcatcagact tatcttttgc agcagcatca aatgcagcct tcttattatt
     4921 tacatccgtc tgtgcccgct gggccttaag ccctgccatt tgccacattc ggtgaccagc
     4981 agccattggt tctcgggcaa atcgctcgaa ttgttttatt tcagcctttg catcagcaag
     5041 agttttattc gcagcatcaa gttcactttt acgagaatta taaacctgaa cagctttagc
     5101 ctgtcgttcc tggtttctgg caacatcttt attagcctga tccagttctg cgcgggcctg
     5161 ttcataattg cgttcagcca cttctacagg atgtgtgtca ttccattcct gctggaggcg
     5221 attttcctca tcctgacgtt gttttacctg tgcaggggta agaacatctg tcactgatac
     5281 ataaatcggc ttctgcccac tttctttcgg aaaacggatc accgcttcat gtgaaccacc
     5341 accaaaggta aatcctgcag gaacggcagt ccggccttta tcctccgtaa taccacgggg
     5401 aagggttgtt gacaccggaa ggcctttgac agtgctgact gtcagagaag ggacaccagg
     5461 aaatgatgcg tgaaatacac ctggagtacg tgttggtttg gcattcacaa caggcacact
     5521 cataggcaca cctgcaacta cggcaatatg ctgtcgtgtg tccttcacaa catctgtcac
     5581 acgtttcgtc acgctgacag ttgcctggtc cagcggaagt gtactgacct gaacattcgt
     5641 cactgtttct gccggtaatg acgtcacaat ctttgacatc atattcgggt catcttttgc
     5701 tatttcagat ggcagaatgc cgtaaagcgc aataccccat gcgctgaatt taaacggacc
     5761 tttcagggct gcaaagatat cagctattgc cgcagataaa gcctcaccag agacggcgat
     5821 acccagcgtt ccggcaccag gagttgccaa agcaggaaaa ccaaatgcca ctgctgctac
     5881 ggatgaattg ctgccaccac cggaattacc attccccccg ccattaccat tgccggagcc
     5941 acctccccag tgaacaccac taccggaacc gccaccccat ggattatttt ccgaactcca
     6001 tcctgaaccg tcagaagccc caccatttcc accaagcccc gtagggccgc cattaatgtt
     6061 accacctgtg ttatgtgcgc cactgttgtg gtcgcgtcca tctccaccgc tcataaaaat
     6121 tccctctttt aagcgttaaa acaattgatt acatagttaa tactgtacat aaaaccactg
     6181 gttttatgta cagatcaaaa agtactcgcc attatttttt ct
//
LOCUS       DAZKHK010000205         6207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKHK010000205 DAZKHK010000000
VERSION     DAZKHK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.206"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..311
                     /locus_tag="ACHOS5_004772"
     CDS             36..311
                     /locus_tag="ACHOS5_004772"
                     /inference="COORDINATES: protein motif:HMM:NF015301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFP9779464.1"
                     /translation="MLLRNGMGLLSKDRYTSPGSMSIEHFKCLVEISSINSQKTIMAM
                     EDYFVHGKTRKEACERNNVAQSYFSISVKKFIKISNAVAQASKFYRR"
     gene            356..862
                     /locus_tag="ACHOS5_004773"
     CDS             356..862
                     /locus_tag="ACHOS5_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9779465.1"
                     /translation="MLKSTLGIAIAFSLCAVAHAANQGQGVVNFKGTVIDAPCGIDPD
                     SADQTIDFGQISKSHLDADGISVKKDLNIKLVNCEPNKSVKVTFSGATIASAANELGT
                     AGDTGTAVVISGQDGKLVSFGTEGSAQSLQEGENTLHYSSWVKKATGGTVKEGDFTAV
                     ANFNLSYQ"
     gene            948..1535
                     /locus_tag="ACHOS5_004774"
     CDS             948..1535
                     /locus_tag="ACHOS5_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9779466.1"
                     /translation="MLRRMILSVLFLWCAVAQALPSASFPPPGMTLPEYWGEEHVWWD
                     GRASFKGQVIAPACTLSMEDAWQEIDMGTTPLRDLQNSPAGPEKKFRLRLRNCELAGA
                     GKQVYTATRVRVTFDGIPGETPDKFSLTGQAEGINLQIMDNYGYPARAGKSMPPLILR
                     GNEDGLDYSLRIVRNGYPLKAGDYYAALRFKLDYE"
     gene            1656..4166
                     /locus_tag="ACHOS5_004775"
     CDS             1656..4166
                     /locus_tag="ACHOS5_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9779467.1"
                     /translation="MKKNKSTFTINFITYSLMLSLAGVPVYAVDFNTDVLDAADRQNI
                     DFSRFSRAGYIMPGQYQMEIRVNGQDISPSAFQIAFLEPPFSDSDNEKPLPEPCLTPE
                     IVSRMGLTEASQEKVTYWNNGQCADFRQLSGVEIRPNPAEGMLYINMPQAWLEYSDAS
                     WLPPSRWDNGIPGLLFDYNINGTVNKPHQGKQSQSLNYNGTAGANFGAWRLRADYQGN
                     LNHTTGSAQGTDSQFTWSRFYMYRAIPRWRANLTLGENYINSEIFSSWRYTGASLESN
                     DRMLPPKLRGYAPQVSGIADTNARVVISQQGRILYDSTVPAGPFTIQDLDSSVRGRLD
                     VEVIEQDGRKKTFQVDTAYVPYLTRPGQVRYKLVSGRSRNYEHSMEGPVFAAGEASWG
                     ISNTWSLYGGSIVAGDYNALAVGLGRDLSEFGTVSADVTQSVARIPGYDTKQGKSWRL
                     SYSKRFDEVNTDITFAGYRFSERNYMTMDQYLNARYRNDFTGREKELYTVTLNKNFED
                     WKASVNLQYSHQTYWDRRTSDYYTLSVNRYFDAFSFKNIALGISASRSKYQNRDNDSA
                     FVRLSVPWGTGTASYSGSMSNDRYTNTVGYSDTLNNGLSSYSLNAGVNSGGGQPSQRQ
                     MSAYYNHNGSLTNLSASFSAVENGYSSFGMSASGGATVTMKGAALHAGGMNGGTRLLV
                     DTDGVGGVPVDGGRVYTNRWGIGVVTDVSSYYRNTTSVDLNKLPEDMEATRSVVESVL
                     TEGAIGYREFEVLKGSRLFAVLRMSDNSYPPFGASVTNAKGRELGMVADSGLAWLSGV
                     NPGETLNVGWDGRTQCVVDIPAHPDPAQQLLLPCRQVK"
     gene            4235..4966
                     /locus_tag="ACHOS5_004776"
     CDS             4235..4966
                     /locus_tag="ACHOS5_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9779468.1"
                     /translation="MQKMKPALKKTLMAVACLSAAPAAQAAVSLDRTRAIFNGDEKSM
                     TLNIANDNKQLPYLAQAWVENEKKEKITTGPIIATPPVQRLEPGSKSMVRLTSTPDIS
                     RLPQDRESLFYFSLREIPPKSDKANVLQIALQTKIKLFYRPESIKAKPNAVWQDQLVL
                     NKTSGGYRIDNPTPYYVTVIGIGGSEKQAREGEFDAVMLAPKSTQMVKSGTYNTPYLS
                     YINDYGGRPVLQFSCGGSRCTAVKK"
     gene            5002..5565
                     /locus_tag="ACHOS5_004777"
     CDS             5002..5565
                     /locus_tag="ACHOS5_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus-assembly fibrillin subunit"
                     /protein_id="HFP9779469.1"
                     /translation="MNRATIGFYLAVVLGSCSMNGVSQADELLTRDDFFVADESRHQW
                     VTEHNGRTGALNVKGALVSSPCILDTPEVNFPLQKYKGRYVLNLKVSRCGDGHSDIPE
                     RQSTEHGNIVVKQSAVLKTGKDQILLSAWRNGGLSRKNLQHGDNQITYYINEAQYQKI
                     AKTQMPVTAHKTSDSNRGVLHLNIMYE"
     gene            5649..6167
                     /locus_tag="ACHOS5_004778"
     CDS             5649..6167
                     /locus_tag="ACHOS5_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9779470.1"
                     /translation="MRITVFLLTFLSFLSDLWAVDIPINITGTIIIPPCQINNSNPVD
                     VDFGNIRVSELDTKEHIKVVSFPVYCPYHQGEAYVKMTGQSMTGKDNVLATNIDGLGI
                     ELYQGGEGTGNHLILSSGSSGYGYEVINALSEKNVERTTFTFTAKIYKAEGVTINSGE
                     FSASALINIVYL"
BASE COUNT         1672 a         1314 c         1660 g         1561 t
ORIGIN      
        1 ttatttgttt tcttgcatga gtgtgggtgt aagggatgtt attacgtaat ggaatgggat
       61 tgttatctaa ggatagatat acctctccag gtagtatgtc tattgaacat ttcaagtgcc
      121 ttgttgagat ctcatcaata aatagtcaaa aaaccattat ggctatggaa gattactttg
      181 ttcatggcaa gaccagaaaa gaagcatgtg aaaggaacaa tgtcgcacag agttattttt
      241 caatcagtgt aaaaaagttt attaagataa gtaatgcggt tgctcaggct tccaaatttt
      301 atagaagata atttttagtg gtagaaagta gtctagtgat aggagtgtta tctttatgtt
      361 aaaatcaact ttggggattg ctattgcttt ttcattgtgt gctgttgcac atgcagccaa
      421 tcaagggcaa ggtgttgtta attttaaagg gacggtaatt gatgcaccat gcggtattga
      481 tccggactca gcagatcaga ctattgactt cggtcagatt tctaaatcac atttagatgc
      541 tgatggtatt tctgttaaga aagatttaaa tatcaagcta gttaactgtg agccaaataa
      601 aagtgtcaaa gtaacttttt ctggagcaac gattgctagt gctgcaaatg aactggggac
      661 ggccggtgat actggtactg cggttgtgat ttccggtcag gacggtaagc tagtttcttt
      721 tggtactgaa ggttctgctc aaagtttaca ggaaggtgaa aatacgctgc attattcttc
      781 atgggtaaaa aaagctactg gtggtactgt aaaagaaggt gattttactg cagttgctaa
      841 ctttaatctg agttatcagt aagtaacatt atccggtttt aaccatctca tacacagacc
      901 ctgggacttc agttccaggt cttgccgata agattaagga cgactgcatg ttacgacgca
      961 tgatcctttc ggtactgttt ctctggtgtg cagtggctca ggctctgcca tcagcttctt
     1021 ttccaccacc ggggatgact ttgcccgagt actggggaga agaacatgtc tggtgggatg
     1081 ggcgggccag ctttaaaggg caggttatcg ctcctgcctg tacgctgtcc atggaggatg
     1141 cctggcagga aattgatatg ggaaccacgc cactcaggga cttacagaac agtcctgccg
     1201 gtcccgagaa gaaattccgg ctacggctac gcaactgtga gcttgcaggt gcaggaaagc
     1261 aggtctatac ggcaacccgg gtgcgtgtga cttttgatgg cattcccgga gaaacaccgg
     1321 acaaattctc gctgacaggt caggctgaag gtataaatct gcagattatg gataattacg
     1381 gatatccggc gagagcagga aaaagcatgc cacctttaat tctcaggggg aatgaagacg
     1441 ggcttgatta ttctcttcgc attgtcagaa atggttatcc attaaaggct ggcgattact
     1501 acgctgcatt gcgttttaag ctggactacg aataaataca accggagtag ccgggctcac
     1561 tgctatcagc gacagcgtta ataatacatt cctgaatgtt gaagagggtt agttgcgccc
     1621 ctcaaagtgt ttttgtatta aatcggctgc atataatgaa aaaaaataaa tcgactttta
     1681 ccatcaattt tattacgtat tcattgatgc tttctctggc aggagtgcct gtctatgccg
     1741 tcgattttaa tacggatgtt ctggatgccg cagacagaca gaatattgat ttttcccggt
     1801 tctcccgggc cggctatatt atgccaggac agtatcagat ggaaataagg gtgaatggcc
     1861 aggatatttc tccatcggca tttcagattg cgtttcttga accacctttt tcggattcgg
     1921 ataacgagaa accgttacca gaaccctgcc tgacaccgga gatagtcagc cggatgggac
     1981 tgactgaggc gtcacaggaa aaagttacgt actggaataa cggacaatgt gcggatttcc
     2041 gacagttatc cggagtggag atacgcccga atccggcaga agggatgctg tacatcaata
     2101 tgccacaggc ctggttggaa tattccgatg catcctggct gccgccgtcc cgctgggata
     2161 acggtattcc cggactgttg tttgactaca acatcaacgg gacagtgaat aagccgcatc
     2221 aggggaaaca gtcccagtcg ctgaactata acggaactgc gggggctaac ttcggtgcct
     2281 ggcgtctgcg tgcggactat cagggcaacc tgaatcacac caccggcagt gcgcagggga
     2341 cggacagcca gttcacctgg agccgtttct acatgtaccg cgccatcccg cgctggcgcg
     2401 caaatctcac actgggtgag aactacatca attctgagat tttcagctcc tggcgctaca
     2461 ccggtgccag tctggaaagt aatgaccgca tgctgccgcc aaaactgcgc ggttatgcac
     2521 cgcaggtctc agggattgcg gatacgaatg cgcgtgtcgt gatttcgcag cagggacgca
     2581 tactctatga ctccaccgtg ccggcaggtc cgttcaccat tcaggacctg gacagctccg
     2641 tgcgtggccg cctggatgtt gaagttatcg agcaggacgg gcggaaaaaa acgttccagg
     2701 tggacactgc atatgtgccg tatctgaccc gtccgggcca ggttcgttac aagctggttt
     2761 ccggacgttc acgtaattat gagcactcca tggaagggcc ggtctttgct gccggtgagg
     2821 catcctgggg tatcagtaac acgtggtcgc tttatggagg cagtattgtt gccggggatt
     2881 ataacgccct ggctgtgggg ctgggccggg acctgagtga gttcggcact gtttccgctg
     2941 atgtaaccca gtccgtggcc cgtataccgg gttatgacac aaaacaggga aaatcctggc
     3001 gtctgagcta ttccaaacgt tttgatgaag tgaatacgga tatcactttt gcgggttacc
     3061 gcttctctga acgtaattac atgacgatgg accagtacct caatgcccgt taccggaatg
     3121 attttacggg acgtgagaag gagctgtaca ccgtcacact caacaagaac ttcgaggact
     3181 ggaaggcatc ggtcaacctg cagtacagcc atcagactta ctgggaccgc cggacatctg
     3241 actattacac gctgtcggtg aaccgctact tcgatgcgtt cagctttaag aatatcgcgc
     3301 tgggaatatc tgcatcccgt tcgaaatacc agaaccggga taacgattcg gcgtttgttc
     3361 gtctgtccgt gccgtgggga accggtacgg ccagctacag cggcagcatg agcaacgacc
     3421 gttacaccaa tacggtgggt tacagcgaca cactgaataa cggcctgagc agttacagcc
     3481 tgaatgccgg cgtgaacagt ggcggtggtc agccgtcaca acggcaaatg agtgcctatt
     3541 acaaccataa cggttccctg acaaacctgt cagccagttt ctctgccgtg gagaacggat
     3601 acagctcatt cgggatgagt gcatcggggg gggcgacggt cacgatgaaa ggagccgcgt
     3661 tacatgccgg agggatgaac ggaggaaccc gtcttctggt ggataccgat ggtgtcggag
     3721 gtgtgccggt tgacggtggt cgggtttata ccaaccggtg gggaattggt gtggtgacgg
     3781 atgtgagcag ttattaccgc aacaccacat cagtagacct gaacaagctg ccggaagata
     3841 tggaagccac ccgctcggtg gtggagtcgg tcctgacgga aggggccatt ggttatcgtg
     3901 agttcgaagt tctgaaaggt agccgtctgt ttgctgtgtt gcggatgtct gataacagct
     3961 atccgccctt tggtgccagc gtgacaaatg cgaaaggccg tgaactgggg atggtggcag
     4021 acagcggtct ggcctggctg agcggagtga atccgggaga aaccctgaac gtcggctggg
     4081 acggccggac acagtgtgtg gtggacatcc cggcacatcc ggacccggca caacagttgc
     4141 tgctgccctg tcgtcaggtg aagtgacgtt atacacgccg gcgatcgtct gtatatcccg
     4201 gcagaaaaat gtaaagaaac agagagcaga aaatatgcag aaaatgaaac cggcgctgaa
     4261 aaaaacgctg atggcggtag cgtgtctgag cgcagcacct gcagcccagg ctgctgtctc
     4321 cctggaccgt acacgtgcga tttttaatgg cgatgagaaa tcgatgacac tgaatatcgc
     4381 caatgataac aaacagcttc cctatctggc ccaggcatgg gtggagaatg aaaagaaaga
     4441 aaaaatcacc acaggcccga ttattgccac tccgccagta cagcgtctgg aacccggctc
     4501 aaagagtatg gttcgtctga ccagtacacc tgacatcagt cgcctgccac aggacaggga
     4561 atcccttttt tacttcagtc ttcgggaaat tccccctaaa agcgataagg caaatgtgtt
     4621 gcagatagcc ctgcagacaa aaataaaact gttttatcgg cctgaatcca ttaaagcaaa
     4681 gcccaatgcg gtatggcagg accagttggt gctgaataag accagtggag ggtatcggat
     4741 tgataatccg acaccgtatt acgtgactgt aattggcatt ggcggcagcg aaaagcaggc
     4801 cagagaaggt gaatttgatg ctgtgatgct ggcaccgaag tcaacgcaga tggtgaaatc
     4861 aggaacatac aacacaccat acctgagtta catcaacgac tacggcggac gtccggtgct
     4921 gcagttctcc tgtggtggta gccgctgtac cgcagtgaag aaataagaaa gcaggaaaag
     4981 aattgtccgg aggtgtcgga gatgaacagg gcaacgatag gattttatct ggcagttgtg
     5041 ctggggagtt gcagtatgaa cggtgtctca caggcggatg aattgttgac gcgggatgat
     5101 tttttcgtag cagatgaaag ccgtcatcag tgggtcactg agcacaatgg gcggacagga
     5161 gctctgaacg tcaaaggtgc attagtgtca tctccgtgca tactggatac gccagaagtt
     5221 aacttccccc tgcagaaata taaagggaga tatgtactca atcttaaggt atcacgttgt
     5281 ggtgatgggc attctgacat accggaacgc cagtcaacag agcatgggaa tattgttgtg
     5341 aagcaaagcg ctgtgttgaa aacaggcaaa gatcaaattc tgctatccgc atggcgaaac
     5401 ggtggattaa gtagaaagaa tttacaacat ggtgataatc agatcactta ttatataaat
     5461 gaagctcaat atcaaaagat agctaaaaca caaatgcctg ttactgcgca taaaacatct
     5521 gacagtaaca ggggggtact gcatttgaat ataatgtatg aataggcaca gtacgctggc
     5581 atcattatta tctcgttgtg tcttaataac catcttgtta cctccttgtt ttatatcagg
     5641 agaaagattt gagaataaca gtctttttat taacgttcct ttccttttta tctgatttat
     5701 gggcggttga tattccaatc aacatcacgg gaacgattat aattccgcca tgccagataa
     5761 ataacagtaa tcctgttgat gttgattttg gtaatatcag ggtgagtgaa cttgacacaa
     5821 aggaacatat caaagtagtt agtttcccgg tttattgtcc atatcatcag ggcgaggcat
     5881 atgtaaagat gactggacag agtatgaccg ggaaagataa tgttctggcg acaaatattg
     5941 atggacttgg cattgaactt tatcaggggg gggagggaac tggaaaccat cttatcttaa
     6001 gtagtggctc atccgggtat ggatacgaag ttattaatgc gctctctgaa aaaaatgttg
     6061 agaggacgac gttcacgttt acagcaaaaa tatataaggc tgaaggtgtt acaattaata
     6121 gcggggagtt tagtgccagt gcacttataa acatagttta tttgtgagtt tagtttttag
     6181 agattacata tgaaatatgt caaaaaa
//
LOCUS       DAZKHK010000206         6173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKHK010000206 DAZKHK010000000
VERSION     DAZKHK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.207"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            394..1062
                     /locus_tag="ACHOS5_004779"
     CDS             394..1062
                     /locus_tag="ACHOS5_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP9779471.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFSQGKTTQTEQQSQIQPQFTAEEIILLCYMQLWMEKAQNISKHLYP
                     IMKELNSSYTNKLYDIAFETIYMVTKNRDALLREAARLDQSSFVIQRARPMLKSLRAR
                     QFEF"
     gene            1317..1949
                     /locus_tag="ACHOS5_004780"
     CDS             1317..1949
                     /locus_tag="ACHOS5_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFP9779472.1"
                     /translation="MSTQLVFHNVQFQTVQRDGLLWLSAPQIASALQYADQSSVTRIY
                     ARHADEFTNGMTASVKLTDPKGDKQETRIFSLRGAHLIAMFARTPIAKEFRRWVLDIL
                     DREAEKSAIPPQPHIQPQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSDYYGK
                     LFDLAYETRHITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            2004..2123
                     /locus_tag="ACHOS5_004781"
                     /pseudo
     CDS             2004..2123
                     /locus_tag="ACHOS5_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
     gene            2137..2775
                     /locus_tag="ACHOS5_004782"
     CDS             2137..2775
                     /locus_tag="ACHOS5_004782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779473.1"
                     /translation="MVEIGNRYGLLVVLRKGDPYINPRGKVGESRWVCRCDCGKEKLI
                     YRSALTTGRTKSCGCLVKKSAALRCANNGKPRKRKGAYSSWAQMKSRCNNPETPGYKN
                     YGGRGIRVCERWNSFDLFLEDMGERPDGCSIDRIDVNGDYCKENCRWATDREQKLNTR
                     RTRNLTAYGKTMTIKEWSEETGIPYSTITVRLMRGKTIEEALSNGKTKVSSD"
     gene            2747..3148
                     /locus_tag="ACHOS5_004783"
     CDS             2747..3148
                     /locus_tag="ACHOS5_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9779474.1"
                     /translation="MERQRYHLINESVRANALNAVKSAPDKFIIDIRKPTRKLSQNAK
                     FHAMVADISRQVQWCGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRESSA
                     QMSVKRMASLIEYTTSWAVEQGIRFTDRRYE"
     gene            3145..3321
                     /locus_tag="ACHOS5_004784"
     CDS             3145..3321
                     /locus_tag="ACHOS5_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9779475.1"
                     /translation="MRRKRRSITDIVCENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            3324..3725
                     /locus_tag="ACHOS5_004785"
     CDS             3324..3725
                     /locus_tag="ACHOS5_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779476.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            3685..3894
                     /locus_tag="ACHOS5_004786"
     CDS             3685..3894
                     /locus_tag="ACHOS5_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9779477.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCSDCCAELMSDPNSSMYEEEDDE"
     gene            3887..4099
                     /locus_tag="ACHOS5_004787"
     CDS             3887..4099
                     /locus_tag="ACHOS5_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779478.1"
                     /translation="MSDYLKWYLCYRWLIKFAVKDWMTADANKLKQRKDYYYARMKEN
                     YCSIRTRIFIKKDLQSILQLRGKVNG"
     gene            4092..4382
                     /locus_tag="ACHOS5_004788"
     CDS             4092..4382
                     /locus_tag="ACHOS5_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9779479.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGSWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            4379..4741
                     /locus_tag="ACHOS5_004789"
     CDS             4379..4741
                     /locus_tag="ACHOS5_004789"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9779480.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLSITIIAEPPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRVGIKITELENI"
     gene            4741..4935
                     /locus_tag="ACHOS5_004790"
     CDS             4741..4935
                     /locus_tag="ACHOS5_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFP9779481.1"
                     /translation="MNAKIQTIPELLICTRGNQTEVARILNCNRATVRKYIDDKDAKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            4928..5308
                     /locus_tag="ACHOS5_004791"
     CDS             4928..5308
                     /locus_tag="ACHOS5_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9779482.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDGTIGKKLQKA
                     EGVVEGMLMLLGVRLKMDRYVQRL"
     gene            5439..5732
                     /locus_tag="ACHOS5_004792"
     CDS             5439..5732
                     /locus_tag="ACHOS5_004792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779483.1"
                     /translation="MDIEDFPEAEKNDLSLWSDDILNIDWFVILRELLKYQHRLKINH
                     SELALLANFVSFHQDVDSQTLPSISLFATRMRASRAIIEEGFQRLEEKGCCRR"
     gene            5717..6031
                     /locus_tag="ACHOS5_004793"
     CDS             5717..6031
                     /locus_tag="ACHOS5_004793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779484.1"
                     /translation="MLQKISYAQIGHDYDLRNVYDIKPLIRKLIEVLKTEYDKKHTCP
                     LCGKIAVSDEEIEKKLEFRICWNKKRPQSWCRLCRSPKQRRLNTLLCGKKRARKSPPE
                     VV"
BASE COUNT         1930 a         1350 c         1468 g         1425 t
ORIGIN      
        1 aagaacagta aagttttttt catggttagt ctctataatt gattgctaac tacagttaac
       61 catactccac gcaattactg taattatttt cggtggttat ctcagtacac agtaacacct
      121 gaccaggttg tcaacgctcg catcaaataa tgttgacaag ccaatgaaat ctgtcggata
      181 ctcagcttta ggtgctcaaa acacctcagc aagtagcggt taccgcaccc gacagacatg
      241 cggttttttg tgtccagtct tcttggttta tgaccgggcg tgcggctaat acaataccag
      301 caatgggaat atgcccgccg actacttgcg gttttgagcg cccggtcacc ctctcaaaag
      361 gggtaaatca aaatatcagg taggacgcaa agcatgaaaa ccatctccgt ggagtccctc
      421 tccataattt catttagtaa tatacctgta gtgactaccg agcttcttgc cagcttatat
      481 ggcacagaac cagattacat ccgaaaaaat ttcaatcgga attctggacg atttgttatc
      541 ggtaagcact actttttact tgagaatgag gagttgcggg aatttaagca cagcatgtct
      601 ttaagacctt ctgtgaaaat cgcccgtaac gttcgctccc tcatcctctg gacagaacgc
      661 ggcgcagccc gtcacgcaaa aatgctcgaa actgatcagg cgtgggaagt attcgaaaaa
      721 ctggaagact gctatttcag ccagggaaaa acaacacaaa ccgaacagca gtcgcagatt
      781 cagccacaat tcacagccga ggaaatcatc ctcctttgct acatgcagct ctggatggaa
      841 aaagcccaga acatcagcaa acacctgtat cccattatga aagagctgaa ctcctcatac
      901 acgaacaagc tgtatgacat tgcgtttgaa accatctaca tggtgacgaa gaacagagac
      961 gcgctactgc gggaggcggc acggcttgac cagtcaagtt tcgttatcca gcgggccaga
     1021 ccaatgctga aaagcctgcg ggcaagacaa ttcgagtttt aaggccagat ttgacaaatc
     1081 cgcattagcg gggatatatt ccgctccatg gtgctgaaca caccttcgaa agcggaaacc
     1141 gcgcccgtca gtcatgcgga ttttttatgt ccatttttca gatatggtcg ggtagcgcgt
     1201 ataccgaaaa acagccgcaa ggctaaggat gcgggccgaa ctttcgacgg tgttcaagta
     1261 cccgaccgcc ctgctgaaca cggggtgatc tgaacgaatc gaaaggacat aaaactatga
     1321 gcactcaact tgtattccac aatgttcaat tccagaccgt tcaacgagat ggtctactgt
     1381 ggttgtctgc cccccaaata gccagcgcgt tacaatatgc agatcaaagc tcggtaacac
     1441 gcatctatgc ccgtcacgca gatgaattca caaacggtat gacagcctcg gtcaaattga
     1501 ccgatcctaa aggggacaag caagagactc gcatcttctc attacgcggc gctcacctaa
     1561 tagcgatgtt tgctcgaact ccaatagcaa aagagtttcg tcgctgggta ttggatattc
     1621 tggaccgtga agctgaaaaa agcgctatac cgccacaacc tcatattcag ccgcaattca
     1681 cagcagaaga catcatcctg ctctgctacg ttcaactctg gatggaaaaa gcacaacaac
     1741 tcagtaaaca gctatacccc gtcatgagag aactgaactc tgattactac gggaaactgt
     1801 ttgatctggc ctacgagact cgtcatataa ccaaccggac tcgcgacaca ttgctccgcg
     1861 aagcagcaaa acttgatccc gccaactata tggtgcatag ggccagaagc atgctggcgc
     1921 aactacgggc aagacaattt gaattctgaa actaaaggag cttcggctcc ttttttgttg
     1981 ggaaaatcca atgagaggga ataatgaacc agactatctt cctccgaagt aagcagcagc
     2041 aacaattcgc cattaacgcc atccttgcaa caactctcga taaagacaag ccagtcacca
     2101 tccgcattac tgactacaag cgcaaggaat tactatatgg ttgaaattgg caacagatac
     2161 ggactcctgg tggtattacg aaaaggagat ccatatataa acccaagagg taaagttgga
     2221 gaaagccgat gggtttgtcg gtgtgattgt ggaaaggaaa aacttattta tcgttcggcc
     2281 ctgacaacag gtagaactaa atcatgcgga tgtcttgtaa agaaatcggc agcattgaga
     2341 tgtgcaaata acggaaaacc caggaaaagg aagggcgcat attcttcatg ggcccaaatg
     2401 aaatctagat gcaataaccc cgaaacacca ggatacaaaa actatggagg gagggggatt
     2461 cgcgtttgtg aacggtggaa ttcatttgat cttttcctgg aagacatggg agagaggcct
     2521 gacggatgca gcattgatcg tattgatgtc aatggtgatt actgcaaaga gaattgccga
     2581 tgggctactg accgagagca aaagctcaac accagaagaa cgcgaaatct tacagcgtac
     2641 gggaaaacaa tgaccattaa agaatggagt gaagaaacag gcatacccta ctcaacgatt
     2701 acagtaaggc taatgagagg aaaaaccata gaagaggcgc tatctaatgg aaagacaaag
     2761 gtatcatctg attaatgaat ccgtcagagc taacgcatta aacgcagtaa aatccgcccc
     2821 cgataaattc atcatagaca tccgcaaacc aacaagaaaa ttatctcaaa atgccaaatt
     2881 tcacgcgatg gtcgctgata tctcccgaca ggttcagtgg tgcggcagat ggctaaaacc
     2941 agaacagtgg aaagttttgt taatcagcgg tcatgccgtg gcgacaaaac aggaagctga
     3001 tgttttgcca ggtcttgaag gtgaatacgt caatatccgc gaaagcagtg cgcagatgag
     3061 cgtgaagcgt atggcaagcc tgattgagta cacaacttcc tgggctgtgg agcaggggat
     3121 cagatttacc gacaggaggt atgaatgaga cgaaagcgac gaagcatcac cgatatagtc
     3181 tgtgaaaact gcaaatacct tccaacgaaa cgctccagaa ataaacgcaa gccaatccca
     3241 aaagaatctg acgtaaaaac cttcaactac acggctcacc tgtgggatat ccggtggcta
     3301 agacatcgtg cgaggaaatg acaatgcttt taattcaacc tggatttggc cttagcatca
     3361 aaaaagggca catgtttggc gagaaagagt ctcaacgaaa aatggtgtct atccagttgc
     3421 catttatcag tatttattgg ctaaacaggg aggcaacaaa ttattggtat acatgcgcca
     3481 gagcagcatt taacgaccct gactggtttg tgaaaaacca ccacgcagtt cgtcaggcaa
     3541 agagaaaggc caatacgaca tacatgaagg cgtatcgaaa agcatggaaa gaacaccgcg
     3601 atcgatacca gcaagacatg gaaaagcttg aatcagaaaa catggaatta agacgaaagc
     3661 ttggtgaagc aaaacgagac attgatgctt acaagcgact ttttaatggt gaaagccatg
     3721 cttagtccat cccaatctct tcaataccag aaagaaagcg tcgagcgagc tttaacgtgc
     3781 gctaactgcg gtcagaagct gcatgtgctg gaagttcacg tgtgctccga ttgctgcgca
     3841 gaactgatga gcgatccgaa tagctcaatg tacgaggaag aagacgatga gtgattatct
     3901 gaaatggtat ctctgctacc gctggttaat taagtttgct gtaaaagact ggatgacagc
     3961 ggatgccaac aagcttaagc aaagaaagga ctattactac gccagaatga aggaaaacta
     4021 ctgctcaatt cgcactcgca tatttattaa aaaagacctt cagtcaattc ttcaattgcg
     4081 agggaaggta aatggctaac ctacgcaaag aagcgcgcgg cagagaatgc caggtacgta
     4141 tttacggcgt atgcaatggc aatcctgaaa ctacagttct ggcacattac cggatggctg
     4201 gaatttgcgg aacgggaatg aagcctgacg acctgatcgg ctcatgggct tgtagcgcgt
     4261 gtcacgatga aatcgaccga cgcacccata atctcgacaa caaagacgcc agactttacc
     4321 acctggaagg cgtgatcagg acgcaggcga tactactgaa ggaggggaaa attaagtcat
     4381 gaatgaatat gagtttgtgc ttccctggcc gccgacggtg aatacctact ggcgaagacg
     4441 gggaagccag tactacatca gcgataaagg ccagaaatac cgaaaagacg tacagcaaat
     4501 catccggcaa ctcagattag acattttcac taaatcacga cttagcatca caattattgc
     4561 tgaaccacca gattcccgcc gtcgcgacct cgataacatc ctgaaaggtt tactcgactc
     4621 tcttatccac gccggatttg cggaagacga cgagcaattc gatgacattc gcgtaattcg
     4681 cggcgtgaaa gtgcctggcg gtagagtggg gataaaaatc accgaactgg agaacatttg
     4741 atgaatgcta aaattcaaac gatacctgaa ttactgatct gcaccagggg aaatcagaca
     4801 gaagtcgcca gaatactgaa ctgcaatcgt gccacagtca gaaaatacat tgatgataaa
     4861 gatgcgaaaa agcacgccgt cgtcaatgga gtccttatgg ttcatcgcgg atggggtaaa
     4921 gatactgatg cgtgatatcc ggcaggttct tgagcgctgg ggggcatggg cggcaaataa
     4981 ccatgaggat gttacatggt cgcccattgc tgccggattt aagggactga tccccgaaaa
     5041 agtaaaatca cgtccacagt gttgtgacga tgacgcgatg attatatgcg ggtgtatggc
     5101 tcgccttaac aggaacaaca gtgatctgca tgacttgctg gttgattatt acgtgttggg
     5161 ggagacgttc atggcgctgg cacgtaagca tggatgctct gatggaacta ttggtaaaaa
     5221 attacagaaa gcggagggcg tggttgaagg gatgttaatg ttacttggtg tcagactgaa
     5281 aatggaccga tatgtgcagc gcctgtgata gcaggcgctt gtcgttcact atgtgaagta
     5341 tgtaaatttg tgaacgttat gctatttact ttataggaca aggacgtaag agttatctac
     5401 cgtttaaccg cagagataca acaatgaaca ggggtaaaat ggacatcgag gatttcccgg
     5461 aagcagagaa aaacgatttg tctctgtgga gtgatgatat tttaaacatt gactggttcg
     5521 ttattctcag agagcttttg aaataccagc acaggttaaa aataaaccat tcagaattag
     5581 ctctgctggc taattttgtt tcatttcatc aagatgtaga ttcacaaaca ttaccgtcaa
     5641 tatctctttt tgccaccaga atgcgtgcat ctcgtgccat tatagaagaa ggctttcaac
     5701 gcctggagga aaagggatgt tgcagaagat aagttatgct caaatcggtc atgattatga
     5761 tttgcggaac gtttatgaca tcaagccatt aattcgtaag ttaattgagg tgctgaaaac
     5821 cgagtatgat aaaaagcaca cttgccctct ctgcggcaaa atagctgtct ctgatgagga
     5881 aattgagaaa aaattagaat tcagaatttg ttggaataag aaaaggccgc aaagctggtg
     5941 tcgtttatgc agaagcccca aacaacgccg actgaataca ttgctatgtg gtaaaaaaag
     6001 agctcgaaaa tccccaccag aggtggtgtg aagcgtcgcg gaatccctga ttccgctccc
     6061 gattgaacgg gatgctaaaa agtattttac gtacgtaaaa atctgcatat catgataaga
     6121 gtggttacat tgccacgctg cttaacccgc cgatgcgcgg gttttttgta tcc
//
LOCUS       DAZKHK010000207         6170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKHK010000207 DAZKHK010000000
VERSION     DAZKHK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.208"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(29..322)
                     /gene="bor"
                     /locus_tag="ACHOS5_004794"
     CDS             complement(29..322)
                     /gene="bor"
                     /locus_tag="ACHOS5_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFP9779485.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(614..1024)
                     /locus_tag="ACHOS5_004795"
     CDS             complement(614..1024)
                     /locus_tag="ACHOS5_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP9779486.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYAFDNSLLFTESIQNTTQIFPR"
     gene            1310..1516
                     /gene="ybcW"
                     /locus_tag="ACHOS5_004796"
     CDS             1310..1516
                     /gene="ybcW"
                     /locus_tag="ACHOS5_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFP9779487.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(1681..1875)
                     /locus_tag="ACHOS5_004797"
     CDS             complement(1681..1875)
                     /locus_tag="ACHOS5_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9779488.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            2264..2809
                     /locus_tag="ACHOS5_004798"
     CDS             2264..2809
                     /locus_tag="ACHOS5_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9779489.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AAIKWYAERDAEIENEKLRREVEELRQDSETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2784..4709
                     /locus_tag="ACHOS5_004799"
     CDS             2784..4709
                     /locus_tag="ACHOS5_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9779490.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNTMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            4706..4912
                     /locus_tag="ACHOS5_004800"
     CDS             4706..4912
                     /locus_tag="ACHOS5_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP9779491.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4909..>6170
                     /locus_tag="ACHOS5_004801"
     CDS             4909..>6170
                     /locus_tag="ACHOS5_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9779492.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWMPRELPGGRTSFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAHFMGRRKFVASRQASQMFLCWLEEAIA"
BASE COUNT         1541 a         1403 c         1683 g         1543 t
ORIGIN      
        1 tgcccatatc gatgggcagc tcatgcaatt attgtgagca atacacacgc gcttccagcg
       61 gagtataaat gcctaaagta ataaaaccga gcaatccatt tacgaatgtt tgctgggttt
      121 ctgttttaac aacattttct gcgccgccac aaattttagc tgcatcgaca gttttcttct
      181 gcccaattcc agagacgaag aaatggtggg tgatggtttc ctttggtgtt actgctgccg
      241 gtttgttttg aacagtaaac gtctgttggg cacatcctgt aataagcagg gccagcgcag
      301 tagcgagtag catttttttc atggtgttat tcccgatgct ttttaaagtt cgcagaatcg
      361 tatgtgtaga aaattaaaca aaccctaaac aatgagttga aatctcatat tgttaatatt
      421 tattaatgta tgtcagatgc gaaggatcgt cactgtattc ccggattaac tatgtccgca
      481 gccctgacag ggaaactcct ctgtgggagt gtccgggaat aattaataac gatgcacact
      541 gggtttagcg cgtacacgta ttgcattatg ccaacgcccc ggtgctgaca cggaagaaac
      601 cggacgttat gatttagcgt ggaaagattt gtgtagtgtt ctgaatgctc tcagtaaata
      661 gtaatgaatt atcaaaggca tagtaatatc ttttatgttc gtggatattt gtaacccatc
      721 ggaaaactcc tgctttagca agattttccc tgtattgctg aaatgtgatt tctcttgatt
      781 tcaacctatc ataggacgtt tctataagat gcgtatttct tgagaattta acatttacaa
      841 cctttttaag tccttttatt aacacagtgt tatcgttttc taacacaatg tgaatattat
      901 ctgtggctag atagtaaata taatgtgaga cattgtgacg ttttagttca gaataaaaca
      961 attcacagtt taaatctttt cgcacttgat cgaatatttc tttaaaaatg gcaacctgag
     1021 ccattggtaa aaccttccat gtgatacgag ggcgcgtagt ttgcattatc gtttttatcg
     1081 tttcaatctg gtctgacctc tttgtgtttt gttgatgatt tatgtcaaat attaggaatg
     1141 ttttcaatta atagtattgg ttgcgtaaca aagtgcggtc ctgctggcat tctggaggga
     1201 aatacaaccg acagatgtat gtaaggccaa cgtgctcaaa tcttcataca gaaagatttg
     1261 aagtaatatt ttaaccgcta gatgaagagc aagcgcatgg agcgacaaaa tgaataaaga
     1321 acaatctgct gatgatccct ccgtggatct gattcgtgta aaaaatatgc ttaatagcac
     1381 catttctatg agttaccctg atgttgtaat tgcatgtata gaacataagg tgtctctgga
     1441 agcattcagg gcaattgagg cagcgttggt gaagcacgat aataatatga aggattattc
     1501 cctggtggtt gactgatcac cataactgct aatcattcaa actacttaac ctgtgacaga
     1561 gccaacacgc agtctgtcac tgtcaggaaa gtggtaaaac tgcaactcaa ttactgcaat
     1621 gccctcgtaa ttaagtgaat ttacaatatc gtcctgttcg gagggaagaa cgcgggatgt
     1681 tcattcttca tcacttttaa ttgatgtata tgctctcttt tctgacgtta gcctccgacg
     1741 gcaggcttca atgacccagg ctgagaaatt tccggaccct ttttgatcaa gagcgatgtt
     1801 aatttgttca atcatttggt taggaaagcg gatgttgcgg gttgttgttc tgcgggttct
     1861 gttcttcgtt gacatgaggc tgccccgtat tcagtgtcgc tgatttgtat tgtctgaagt
     1921 tgtttttacg ttaagttgat gcggatcaat taatacgata cctgcgtcat aattgattat
     1981 ttgacgtggt ttgatggcgt agatgcacgt tgtgacatgt agatgataat tattatcatt
     2041 ttgcgggtcc tttccggcga tccgacaggt tacggggcgg cgacctcgcg gtttttcact
     2101 atttatgaaa attttccggt ttaaggcgtt tccgttcttc ttcgccgtaa ctttatgttt
     2161 ttatttgaaa caccccctga aaagaaagga aacgacaggt gctgaaaacg ggctttttgg
     2221 cctctgtcgt ttcctttctc tgtttttgtc cgtggaatga acaatggaag tcaacaaaaa
     2281 gcagctggct gacattttcg gcgcgagtat ccgtactatt cagaactggc aggagcaggg
     2341 aatgcccgtt ctgcggggtg gtggcaaggg taatgaggta ctttatgact ctgccgccgc
     2401 cataaaatgg tatgccgaaa gggatgctga aattgagaac gaaaagctgc gccgggaagt
     2461 tgaagaactg aggcaggaca gcgagacaga tctccagcca gggactattg agtacgaacg
     2521 ccatcgactt acgcgtgcgc aggccgacgc acaggagctg aagaatgcca gagactccgc
     2581 tgaagtggtg gaaaccgcat tctgtacttt cgtgctgtcg cggatcgcag gtgaaattgc
     2641 cagtattctc gacgggatcc ccctgtcggt gcagcggcgt tttccggaac tggaaaaccg
     2701 acatgttgat ttcctgaaac gggatatcat caaagccatg aacaaagcag ccgcactgga
     2761 tgaactgata ccggggttgc tgagtgaata tatcgaacag tcaggttaac aggctgcggc
     2821 attttgtccg cgccgggctt cgctcactgt tcaggccgga gccacagacc gccgttgaat
     2881 gggcggatgc caattactat ctcccgaaag aatccgcata ccaggaaggg cgctgggaaa
     2941 cactgccctt tcagcgggcc atcatgaata cgatgggcag cgactacatc cgcgaggtga
     3001 atgtggtgaa gtctgcccgt gttggttatt ccaaaatgct gctgggtgtt tatgcctact
     3061 tcatagagca taagcagcgt aacaccctta tctggttgcc gacggatggc gatgccgaaa
     3121 actttatgaa aacccacgtt gagccgacta tccgtgatat tccgtcgctg ctggcgctgg
     3181 caccgtggta tggcaaaaag caccgggata acacgctcac catgaagcgt tttaccaatg
     3241 gtcgtggctt ctggtgcctg ggcggtaaag cggcaaaaaa ctaccgtgaa aagtcggtgg
     3301 atgtggcggg ttatgatgaa cttgctgcct ttgatgatga tattgaacag gaaggctctc
     3361 cgacgttcct gggcgataag cgtattgaag gctcggtctg gccaaagtcc atccgtggct
     3421 ccacgcccaa agtgagaggc acctgccaga ttgagcgtgc agccagtgaa tccccgcatt
     3481 ttatgcgttt tcatgttgcc tgcccgcact gcggggagga gcagtacctt aaatttggcg
     3541 ataaagagac gccgtttggc ctcaaatgga cgccggatga tccctccagc gtgttttatc
     3601 tctgcgagca taatgcctgc gtcatccgcc agcaggagct ggactttact gatgcccgtt
     3661 atatctgcga aaagaccggg atctggaccc gtgatggcat tctctggttt tcgtcatccg
     3721 gtgaagagat tgagccgcct gacagtgtga cctttcacat ctggacagcg tacagcccgt
     3781 tcaccacctg ggtgcagatt gtcaaagact ggatgaaaac gaaaggggat acgggaaaac
     3841 gtaaaacctt cgtgaacacc acgctcggtg agacgtggga agcgaaaatc ggcgaacgtc
     3901 cggatgctga agtgatggca gagcggaaag agcattattc agcgcccgtt cccgaccgtg
     3961 tggcttacct gaccgccggt atcgactccc agctggaccg ctacgaaatg cgcgtatggg
     4021 gatgggggcc gggtgaggaa agctggctga ttgaccggca gattattatg ggccgccacg
     4081 atgatgaaca gacgctgctg cgtgtggatg aggccatcaa taaaacctat acccgccgga
     4141 atggtgcaga aatgtcggta tcccgtatct gctgggatac tggcgggatt gacccgacta
     4201 ttgtgtatga acgctcgaaa aagcatgggc tgttccgggt gatccccatt aaaggggcat
     4261 ccgtctacgg aaagccggtg gccagcatgc cacgtaagcg aaacaaaaac ggggtttacc
     4321 ttaccgaaat cggtacggat accgcgaaag agcagattta taaccgcttc acactgacgc
     4381 cggaaggaga tgaaccgctt cccggtgccg ttcacttccc gaataacccg gatatttttg
     4441 atctgaccga agcgcagcag ctgactgctg aagagcaggt cgaaaaatgg gtggatggca
     4501 ggaaaaaaat actgtgggac agcaaaaagc gacgcaatga ggcactcgac tgcttcgttt
     4561 atgcgctggc ggcgctgcgc atcagtattt cgcgctggca gctggatctt agtgcactgc
     4621 tggcgagcct gcaggaagag gatggtgcag caaccaacaa gaaaacactg gcagattacg
     4681 cccgtgcctt atccggagag gatgaatgac gcgacaggaa gaacttgccg ctgcccgtgc
     4741 ggcactgcat gacctgatga caggaaaacg ggtggcaacg gtacagaaag acggacggag
     4801 agtggagttt acggccactt ccgtgtctga cctgaaaaaa tacattgcgg agctggaagt
     4861 gcagaccggc atgacacagc gacgcagggg acctgcagga ttttatgtat gaaaacgccc
     4921 actattccca cccttctggg gccggacggc atgacatcgc tgcgcgaata tgccggttat
     4981 cacggcggtg gcagcggatt tggagggcag ttgcgggcgt ggaacccacc gggtgaaagt
     5041 gtggatgcag ccctgttgcc caactttacc cgtggcaatg cccgcgcaga cgatctggta
     5101 cgcaataacg gctatgccgc caacgccatc cagctgcatc aggatcatat cgtcggatct
     5161 tttttccggc tcagtcatcg cccaagctgg cgatatctgg gcatcgggga ggaagaagcc
     5221 cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc
     5281 tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg
     5341 cacgccttta acggtgaact gttcgttcag gccacctggg ataccagtcc ctcgcgactg
     5401 ttccggacac agttccggat ggtcagcccg aagcgtatca gcaacccgaa caataccggc
     5461 gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggcgcggc gctgggatat
     5521 tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg gatgccccgt
     5581 gagttacccg gcgggcgcac ttcgttcatt cacgtttttg aacccgtgga ggacgggcag
     5641 acccgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg
     5701 cagaacacgc agctgcagag cgccattgtg aaggcgatgt atgccgccac cattgagagt
     5761 gagctggata cgcagtcagc gatggatttt attctgggcg cgaacagtca ggagcagcgg
     5821 gaaaggctga ccggctggat tggtgaaatt gccgcgtatt acgccgcagc gccggtccgg
     5881 ctgggaggcg caaaagtacc gcacctgatg ccgggtgatt cactgaacct gcagacggct
     5941 caggatacgg ataacggcta ctccgtgttt gagcagtcac tgctgcggta tatcgctgcc
     6001 ggactgggtg tctcgtatga gcagctttcc cggaattacg cccagatgag ctactccacg
     6061 gcacgggcca gcgcgaacga gtcgtgggcg cactttatgg ggcggcgaaa attcgttgca
     6121 tcccgtcagg cgagccagat gtttctgtgc tggctggaag aggccatcgc
//
LOCUS       DAZKHK010000208         6099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKHK010000208 DAZKHK010000000
VERSION     DAZKHK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.209"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..821
                     /locus_tag="ACHOS5_004802"
     CDS             <1..821
                     /locus_tag="ACHOS5_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9779493.1"
                     /translation="TVAVLVPSLTDKTSSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     AEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFE
                     ASHKLTEHMLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSC
                     DQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1024..1704
                     /locus_tag="ACHOS5_004803"
                     /pseudo
     CDS             1024..1704
                     /locus_tag="ACHOS5_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1721..2029
                     /locus_tag="ACHOS5_004804"
                     /pseudo
     CDS             1721..2029
                     /locus_tag="ACHOS5_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2133..3239)
                     /locus_tag="ACHOS5_004805"
                     /pseudo
     CDS             complement(2133..3239)
                     /locus_tag="ACHOS5_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4006..6054
                     /gene="ireA"
                     /locus_tag="ACHOS5_004806"
     CDS             4006..6054
                     /gene="ireA"
                     /locus_tag="ACHOS5_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFP9779494.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT         1731 a         1350 c         1412 g         1606 t
ORIGIN      
        1 atacagtcgc cgtactggtt ccttccctga cagataaaac cagctcccgc tttatgcagt
       61 cgttgcagca ggtgcttaat aaaaatgaat tccagctact gcttgggtgt catgagtata
      121 atcagctcaa agaagctgaa atcctgatga cgcttcttca aggtaacccg gcggcgttag
      181 ttatttttgg ttctcagttg gctgataaaa ctcaccagat actcgagaaa acaaatatac
      241 ctaccattaa cgttgtcggt tcccctttca gtccggctca aattaccatc gaaacagcat
      301 tttttgaagc atcacataaa ctgaccgaac atatgctgga acagggttat aagaatatcg
      361 gatttatcgg tgcgcatatg gacaaccgcc tgcaacgtca gcaactgaac ggctggcata
      421 aagcaatgct gagctattac cagaactctg atcttgttat caccatgccg ggtgccgcaa
      481 gtttgcaatt ggggcgttac gcattaaacg agatattact gcgacaacca gagctggatg
      541 ctgtgatttg cagccacgaa gagattgcgc tgggtatcat gtttgagtgc cagcggcgtt
      601 tgttaaaaat ccctggaaat atcgctgtag cttgtcttga cggttcagac agttgcgatc
      661 aaacacaccc aaccttgaca tccatccgta ttgactacaa aaaaatgggg acagagacgg
      721 gtaagctgct gattggacta ttgaataata atcatgatga atcagaagag tcgcgtatcg
      781 tgcaattcaa ctaccagata gaattacgtc agagtacatg agctcaaaag gggattattg
      841 caaagttagc tattgtggta gggatagccg tttccggcta ttccccgcgc agagccggat
      901 gtgtgctgct aacgcatcct gtcaacgacg gatgaaaagt tatccactta tatctccacc
      961 aacggcccaa tattgtaccg ttcgtgacac atccatttct acttcaggta tttggcgtag
     1021 tagcgggtgc taaaaatttg ccgcaaaacc gcaatatcag cccgcagagg cgctctccag
     1081 attatccatt cctatattat ctctgcaccg gatgaattac tcgaacaact cagaaatatg
     1141 acacatatgc aacttatcag gactctggga tcctggaggc ctgatgccag tgaatacagt
     1201 aatgttacca acgtttatcg cattacattg aaatcccttg ttcgacgcta tttcgagttt
     1261 catgatgaaa tcaccaactt ggatgtcatg attgcggcaa ttgttgatga actggcacct
     1321 gaactgatta aacgtaatgc tgttggatac gaaagcgctt tgcagttgtt gatctctgcc
     1381 ggagacaatc cctaacggtt aagaaccgaa tcaggttttg cggcgttgtg cggtgtcagc
     1441 cagtttcccc tatcttctgg aaaaacgaac cattaccgac ttaatcgggg tggggagcgt
     1501 gttgcaaata gtgcacttta catcatcact ataggatgtt tacaaactga cgataaaaca
     1561 aaggaatatg tctccaggcg agtagccgaa ggacatacaa aaatggaagc aatacgctgt
     1621 ctgaagcgct atatctcacg cgaagtttat acattactac gaaatcaaaa caggcatgtt
     1681 aactgcatcc cgataacgtc ttgactctta gaagggcgtc caaggcagtc attattcgag
     1741 caggcagttc cggcagttat tgtgtcgata ccagatcagg cagagtatga gccggcgcgg
     1801 aaaatgctgg gataacagct caatggaacg cttcttcagg agtctgaaga acgaatggat
     1861 gccggtggtg ggttacgtaa gcttcagcga ggcagctcac tccataacgg acgatatcgt
     1921 tggatattac agcgcactaa gaccgcacga atataacagt gggttatccc caaacgaatc
     1981 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcagcc
     2041 atcgccagac agctaacatg agttactctt gttctgagct acgtcacctt cagggaactg
     2101 gttatcctca ttctttattt gtgacattgt ggttattgtg cgacaaacgt ggctatcccg
     2161 ccccatatcc cagataaaac cacctgctca cgtgcgacag cgacaattgt aatattgaca
     2221 ttctttcctt tggcctgaag ttttcgagac ctcttgcaaa gcctgagttg agcatcccat
     2281 gctctgtcaa tgacggggcg gggtaaattt tcctgatttt ttgtattgcc ggactgatgc
     2341 gtgtcggatg ccggtaactc cacgctgcct caaccaatag ctttctggca tagctgtttc
     2401 cggctttggt aatgctgccc tgatgtctgc ttccacccga tgaatattct gatggcgtga
     2461 taccaaacca tgacatcagt tgtcgtggat gttcaaaacg ggtcagatca cccagttcag
     2521 aaattaatcc aaccgcagta ataaactgaa tccctcgcat ggcttgctgc gcctctacta
     2581 ccggatacag cctccattcg gtaacggcct ccttcgggcg gattccagcc gttcacattg
     2641 tgcctgccgg tcttctattg tacaacggtg ttcgtcaaaa gctaattgtc gccagggact
     2701 ttcgaatgag tatttgctga gccagtgtcg gtgtgcaggg ccccaatctg ctcgcccgac
     2761 ataatggacc ccatgaacca gaagaaacga tttcagacgt tgccttgcat gccgtaaatc
     2821 atcgcgggca gatgcccatg cccgggccaa atcccggaat gcctcatctt caacgccgga
     2881 tacgtagaca gcagaaagat ctcctgcacg cagtgaacgc acaagtctga tggcatcacg
     2941 gtgtcggttt taccccgctc ccccggtttt ttcgggataa gtgagggggc gcagaccatg
     3001 cagtcaaaac cggatttcac cagccgacga taaagtccat aaccacaggg tcccgcttta
     3061 taaacgatac tgacctgcga tgactttgac ctgatacgtt gataaagatt atgaatgtca
     3121 gtaggtgatg tgctaatttt acccatcagt tcaacaggtt ctgaattgat agcataggag
     3181 acagtaatcg attctttgtg aacatctaat tcgacgtaca acatgttatt gttgttcatg
     3241 ctggcctccg tggtggaaat tccagggggg tagcaacagc tcacccatgc ggctctgaca
     3301 acgatgttaa cccgcattaa atatcccacg ttggacagca tctgtctcac ggaaagtcat
     3361 actgccttac gtgcatattt caccttacgc cttcactgtc tgtcatcgga agtactgatt
     3421 tatattgggg ctttgcactt actgtcattc acgggaaaag atggtggtga acagtacgat
     3481 actgtcttgg ctgttgccga gggaagattc aggagtggta agggggttag tggtgtaaga
     3541 tgagtctgaa gctctgactg gtcagtgacg cgaaactcaa tctaccaggg taaactcccc
     3601 cttgcttttg cgcagaagca gtgggccttg gttcgcttat gtcctgcatc tacaagcgaa
     3661 tgactgaccg gagactccca tatctggtca gtcgcccatt tttattttcc accacatcgg
     3721 acaagcatac cgtaataaac agcaccagtc tcctgttcac tcttgcttga tttactggaa
     3781 tgctgtttat ggagatatcc ggttgaaaga ttttacctct tcgacaggac tataaattat
     3841 acagtaagga gtccctcgta gaaaggggct gattctcatc aagtcggaca ggttcaaagt
     3901 gttacctgaa tgagctaaat gttatttacc ttcctttctg ttcattgtaa attttttctt
     3961 gctaacgcaa atcattatca ttacgatttt agaaaaggga aatttatgaa gaacaaatat
     4021 atcattgctc cgggcattgc cgtgatatgt tctgcagtca tatcatcagg ttatgccagt
     4081 tctgataaaa aagaagatac gcttgttgtt actgcctccg gcttcactca gcaactcaga
     4141 aatgcaccgg ccagtgtttc agtcattact tcagagcaac tgcaaaaaaa accggtttca
     4201 gacctggtcg atgcggtaaa agatgttgaa gggattagta tcaccggtgg gaatgaaaaa
     4261 ccggatatca gtatacgtgg gttaagtggc gattatacat tgattctggt cgatggacga
     4321 cgtcagagtg gtcgggaatc cagaccaaac ggcagcggcg gttttgaagc cggatttatc
     4381 cctcctgtgg aagcaattga gcgtattgaa gttatacgtg gccctatgtc ttccctgtat
     4441 ggttccgatg ctatcggagg agttattaat atcataacca aacctgttaa taaccagaca
     4501 tgggatggcg tacttgggct tgggggaatt attcaggaac atgggaaatt tggtaactca
     4561 accacgaatg acttctacct gtcaggccca ttggttaagg ataaacttgg tcttcagtta
     4621 tatggaggaa tgaactatcg caaggaagat agtatctctc agggaacacc agcaaaagat
     4681 aataagaata taacggcaac actccagttt actccgactg aaagccagaa gtttgttttt
     4741 gaatacggaa aaaataacca ggaacataca ttaacacctg gtgattctct tgatgcctgg
     4801 actatgcggg gaaatcttaa acaaccaaac agtaaaagag aaacacataa ctcacgtagt
     4861 cactgggtag cagcatggaa tgcccagggc gaaatactgc atcctgaaat tgctgtttat
     4921 caggagaaag ttattcgtga ggttaaatct ggtaaaaaag ataaatataa tcattgggat
     4981 cttaattacg agtcaagaaa accggaaata accaacacga tcatagatgc aaaagtgacg
     5041 gcatttctgc cggaaaatgt gctgaccatc ggaggtcaat ttcagcatgc agaactccgt
     5101 gatgactcag ccacaggtaa aaaaacgaca gaaacacagt ctgtttcagt taaacagaaa
     5161 gctgttttta tagaaaatga atatgctgca acggattctc tcgccctgac tggagggttg
     5221 cgcctcgata accatgaaat ctatggcagt tactggaatc caagattgta tgctgtttat
     5281 aacctgaccg ataatctcac tctcaaagga gggatcgcaa aagcatttcg ggccccttcg
     5341 attcgtgagg tcagtcctgg atttggaaca ctgacgcagg gtggtgcctc tattatgtat
     5401 ggaaacaggg acctgaaacc ggagaccagt gtaaccgaag agatcggtat tatttataat
     5461 aatgacagtg gtttttcagc aagcgcgact ctgtttaata cagattttaa aaataagctg
     5521 accagttacg atataggcac aaaagatcca gtcactgggc taaacacttt tatttatgat
     5581 aatgtgggtg aggcaaatat cagaggtgtg gagcttgcaa ctcagattcc tgtgtatgat
     5641 aaatggcgtg tatctgcaaa ctatacattc actgactccc gtcgagaaag cgatgacgaa
     5701 agtctcaatg gcaagtcgct gaaaggggaa cctctggaaa gaactcccag acatgcagcc
     5761 aatgcaaaac tggaatggga ttacactcag gatattacat tttattcatc tctgaattat
     5821 acaggaaaac aaatctgggc agcacaaaga aatggtgcca aggttccccg tgttcgtaat
     5881 ggattcactt ctatggatat tggtatgaat taccagttcc tgccaggcac gctgattaat
     5941 tttgccgtac ttaacgtcac agacagaaag agcgaagata tcgataccat tgatggtaac
     6001 tggcaggtcg atgaaggacg tcgttactgg gctaatgtaa gaatatcctt ctgagagaat
     6061 atagataacc tgaggccgtg cgcctcaggt accagtata
//
LOCUS       DAZKHK010000209         5930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKHK010000209 DAZKHK010000000
VERSION     DAZKHK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.210"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..145)
                     /locus_tag="ACHOS5_004807"
     CDS             complement(<1..145)
                     /locus_tag="ACHOS5_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9779495.1"
                     /translation="MSRITAIISALLICIIVCLSWAVNHYRDNAITYKAQRDKNAREL
                     KLAN"
     gene            complement(142..618)
                     /locus_tag="ACHOS5_004808"
     CDS             complement(142..618)
                     /locus_tag="ACHOS5_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9779496.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVRESEV"
     gene            complement(605..910)
                     /locus_tag="ACHOS5_004809"
     CDS             complement(605..910)
                     /locus_tag="ACHOS5_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9779497.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            complement(1232..1921)
                     /locus_tag="ACHOS5_004810"
     CDS             complement(1232..1921)
                     /locus_tag="ACHOS5_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9779498.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQSYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQDIAKLD"
     gene            complement(1918..2058)
                     /locus_tag="ACHOS5_004811"
     CDS             complement(1918..2058)
                     /locus_tag="ACHOS5_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP9779499.1"
                     /translation="MMFESYMAERLRHRWMRLRLYRFPGSVLTDYRILKNYAKTLKGA
                     AA"
     gene            complement(2055..2417)
                     /gene="rusA"
                     /locus_tag="ACHOS5_004812"
     CDS             complement(2055..2417)
                     /gene="rusA"
                     /locus_tag="ACHOS5_004812"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP9779500.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKG
                     SMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGSE"
     gene            complement(2414..2704)
                     /gene="ybcO"
                     /locus_tag="ACHOS5_004813"
     CDS             complement(2414..2704)
                     /gene="ybcO"
                     /locus_tag="ACHOS5_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9779501.1"
                     /translation="MADLRKAARSRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2697..2867)
                     /gene="ninE"
                     /locus_tag="ACHOS5_004814"
     CDS             complement(2697..2867)
                     /gene="ninE"
                     /locus_tag="ACHOS5_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9779502.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2867..3322)
                     /gene="ybcN"
                     /locus_tag="ACHOS5_004815"
     CDS             complement(2867..3322)
                     /gene="ybcN"
                     /locus_tag="ACHOS5_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP9779503.1"
                     /translation="MNLSQDGIKLHRGNFTAIGQQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHPSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3319..3420)
                     /locus_tag="ACHOS5_004816"
     CDS             complement(3319..3420)
                     /locus_tag="ACHOS5_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779504.1"
                     /translation="MRTHNPNSLLPSQMQKCTCNSLHLTFDLCGGEA"
     gene            complement(3533..4165)
                     /locus_tag="ACHOS5_004817"
     CDS             complement(3533..4165)
                     /locus_tag="ACHOS5_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779505.1"
                     /translation="MKFSRIEQSPTTDEGNYVKIDHDFGYFLDPNKMASIANYEDESA
                     ALLKLMLIHEDFLRVVIENLRPDGSEKYNSIRSYKYFASKLSAAVLLGLPVSLADAMA
                     ELNTIRNKYAHKIEYVITNEEVDKLAESIKNIKLEEINHGGYVHQVVEPILKDGIYAI
                     ALMKELPPDLEENRIRICKLVTSAHCMCLLGAFWLINTLHVKGKLKLSKA"
     gene            complement(4162..4411)
                     /locus_tag="ACHOS5_004818"
                     /pseudo
     CDS             complement(4162..4411)
                     /locus_tag="ACHOS5_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
     gene            complement(4446..4646)
                     /locus_tag="ACHOS5_004819"
     CDS             complement(4446..4646)
                     /locus_tag="ACHOS5_004819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779506.1"
                     /translation="MMYSVTFDETGRVEDIELEREVRAGDRLSLNIDGLDGIYIVMTV
                     SGPIYENVCVPMNIRVQKYWKQ"
     gene            complement(4809..5417)
                     /locus_tag="ACHOS5_004820"
     CDS             complement(4809..5417)
                     /locus_tag="ACHOS5_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFP9779507.1"
                     /translation="MTTQLAFHKTTFTPICHNNRIWLTATEVGLALEYADDKAVQRIY
                     SRHSDEFTDMMTRVVKVTTPRGMQESRVFSLRGAHLIAMFARTPVAKEFRRWVLDILD
                     REIQQSPITKQFTDNELCTLAWLWRAGDMMLTACQNVTPLLQVAEHREAGRFTSIGQE
                     YPQILHKAREILARETAHVKLQPWQDNKWSRVLPHLRQDRLQ"
BASE COUNT         1528 a         1471 c         1338 g         1593 t
ORIGIN      
        1 cgttcgccag cttcagttct ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt
       61 tatcacggta atgattaaca gcccatgaca ggcagacgat gatgcagatg agcagagcgg
      121 agataatcgc ggttattctg ctcatacctc actctctctg accgttccgc cagcttcttt
      181 gaattttgca atcaggctgt cagccttatg ctcgaactga ccataaccag cgcccggcag
      241 tgaagcccag atattactgc aacggtcgat tgcctgacgg atatcaccgc gatcaatcat
      301 aggtaaagca ccacgctctt taatctgctg caatgccact gcgtcctggc ttttggggga
      361 gaagtctttc aaaccaagct gtttacggta agcatcccac cagcgtgaaa gaagctgata
      421 acgtccggcg gctgttgatt tgagtttggg gtgtagcgtg acaagtttgc gagggtgatc
      481 ggagtaatca gtaaacagtt cgccgccaac aataacatca taaccgtggt tacgtgtcgg
      541 ttgtcgcccg ttatccgttc cttctgacca cgccaacata tcgaggaagg ctttacgctg
      601 aggattaaga ttttgcattt ttcacccctg tcagtcgttc ccagaagtac gtcagtgcaa
      661 ccgaacccat cgcaccacta atccccgctg tcgcgagaat catgtaaata ctgaatccac
      721 tttcgatgct gatcaggcca ccaataacac cggtgaatcc tgataccact atttgagcca
      781 gagcatttat ccaactccac gttgctttac tctgcttcac atctatcagg tagcggacca
      841 gaccgcccca acctgcgatg atcagcaaaa cgagccagaa cgctccggca aggctctctt
      901 tttcgtgcat atgaatagcc aatgtttcgc cgccgacaaa aggccgggac gttaaatgtc
      961 agaaatcagg ctcacggggt aatttaacga caaagcacgg agttgatgct ccccacaagc
     1021 ctggaataaa aaagccagca tgtagctggc aacagagggc taagcaatat caactcaaca
     1081 gctgaagaca ccctggctgg ggtaggttgg aaggctactc accgttcaga aacagaaaag
     1141 cccaaggctt taaacctcgg gcttgaattt ggattactgc cagtgcgtac aacattggca
     1201 aaatatcaga tttacataaa atatatgatt tttaatccag ttttgcaata tcttgctgtg
     1261 aaaatgtggt cttttgtttt gaacgtgttt tcgttaaaag caataaagct tggctatcaa
     1321 gctgtagaaa aatgtgtttc attgcaaccc agcgttcagt aaatgtctca gaccagtttt
     1381 ttgatgtcac tcccaccagt gatgccagct cctggtattc ataggtctta cgccctgcca
     1441 gctcgttctt cacatcctgt gccgccagcc agatcaactt cttcaaacgt tccagtgtct
     1501 tacctgcaat tttcctggta cccaacagag tcttaaactc gctccatgcc cactgcgtta
     1561 tggtgacctg atgttcccag cgaacacttt cgctgtaact ccacagcaac cacgctttct
     1621 gatgttcttc gagagacaga accgcgcggc gccatgacga ggttgagaac tcaacctggc
     1681 tgaccagtgc aatggatgaa ccttttgcgt acgactgctt accggaaatc ggcggattat
     1741 ccagcgtaat catcctgcca gttacctcat ccagaatgcg cggcttcttt cgtttgtatg
     1801 taccagtatc aaattgtgca tgctccagcc aggcttcaag ctgtcctttc gttgctccgc
     1861 tcaaatcagc ggtagccaca atgagttgct cgcggacata ctgtaaatat tgggtattca
     1921 tgcggcagct cctttcagtg ttttggcgta attcttcagt atccggtaat cggtcaaaac
     1981 agaaccagga aaacgatata agcgcaggcg catccagcgg tggcgaagac gttctgccat
     2041 ataagactca aacatcattc actccccagt tcggtgatgg tcagctccag cttcccacct
     2101 ttggtaacgg gcatcttcac aacacggtaa tcaacgacct gagcatcatc cagccagaaa
     2161 cctgctttgg tgagtgcgtc aaaagcggct ttttgcagat tatccaggtc acggcgacgg
     2221 cgatccggca tgtggcactc aatacggatt ttcactggca tagccaggcc gatatccagc
     2281 atggagcctt tgatgattcg ggcgacgtta tcacggtatg cctgcccttc tgcgctgatg
     2341 tgcgtgcgcc cgcgattatg ccggtagtag cgattattgc tcggaggcca aggtaatgtg
     2401 atgctgtagg tattcacgcc ttaataaccc cctctttcag ccagataacc tgtgttctcg
     2461 ccataccttc cagcgcgcat tcttttgcat atgcagcatc gacaaaatgt gtgcggcggt
     2521 cgatttcgtc gtggcaggca gaacatgcaa tggtggcaat caagtctggc ggtttggtac
     2581 cggtgccgca caatccagtc agccggatat gtgccagtac tgacgtttca ggattgccat
     2641 tacatacgcc agggattctt acctggcatt cccgactacg cgctgctttt ctcaaatcag
     2701 ccatgattcc tccttgctgc cagtcgcaac cattttttat caaccaggct ggcggtatat
     2761 ccgagcagtg ttggtatttc ggatggcttc agctcaggct tacgcttacg acgatttgat
     2821 actctgtaga tgtgtccgtt catgacacga ataagcggtg tagccattac gcctcctgct
     2881 tgtcgcggag cagctggaac tcgcagctct gcggaatagt caggtggcag ccaatattca
     2941 ccgcccaggc ttcaacctta cacaggaaga catacatctc tccggtatcg agatcggagg
     3001 gatggcgtaa cgactggata gtagtgattt cgccggttac gacatcaacc aggtccttgg
     3061 tttcataacc gaggtatgtg tgtttgagag catcttttac ccatgctgcg gtagcgaacg
     3121 atttccccct gctgatgagg tattcactga tttcgctgta ccacatgtgg ctgagtgcat
     3181 tctgggaaag actgcgtttc tcacgccacg gtttaagcac catgcgaaag catttgcctt
     3241 cctccagata aggctggatc tgctggccga tagcggtgaa gttgccgcga tgtaatttga
     3301 tgccgtcttg tgagaggttc acgcttcacc tccgcagagg tcaaacgtta gatgcaaaga
     3361 attgcaggtg catttctgca tctgtgaagg gagaagagag tttggattgt gtgtgcgcat
     3421 aaacgtcccc gtttagcgca gaagtcaccg aagttgttca ggctccggtg atacaattat
     3481 ggcgaattga ttacggagaa tcaattttgc gaaaatgaca cgattgatct actcaggcct
     3541 tggaaagctt gagcttaccc tttacgtgca gagtgttaat taaccagaag gccccgagca
     3601 aacacataca atgcgcgcta gtgactaact tacaaatacg aatgcgattt tcttcaagat
     3661 caggaggtag ctctttcatc aacgctatag cgtagattcc atccttgaga attggctcaa
     3721 caacttgatg cacgtagcca ccatggttaa tctcttctaa ctttatgttt ttaatcgatt
     3781 ctgcgagttt atcaacctct tcatttgtaa ttacatattc aattttatga gcgtacttgt
     3841 tacgtattgt gttgagctca gccatagcgt ctgcaagcga gacaggcagc cctaacagaa
     3901 ctgcggcaga cagctttgac gcaaaatact tatagcttct tatcgaattg tatttttcag
     3961 agccatcagg tcgtaagttt tcaataacaa cccgaagaaa gtcctcatga attagcatca
     4021 attttaatag cgcggcagac tcatcctcat agttagctat cgaggccatt ttattaggat
     4081 ctagaaaata gccaaagtca tgatcgattt tcacgtaatt accctcatcc gttgttgggc
     4141 tttgttcaat tcgagagaat ttcatttatc ttcctcctgt ttcatatatc tagggtcaga
     4201 tgttttaggc tgggtgatgc tatgctcaat ggagaagtca gacgcgctcc atgaagtaat
     4261 cacaaagatg ctctggttgc tggcacatca ccacttcggc tatgacgaga attttggagc
     4321 gttctttata tgcaacacca gacgcagcaa ggtcaaagtt aacattgtca cgttcctctt
     4381 gcggttttgc tgcaatgttc caatctgaca taaaaatccc ctcgatcatc tgaggggatt
     4441 atacatcatt gcttccaata cttttgaacc cggatattca ttggcacaca aacattttca
     4501 tagatagggc cgctaacagt catgactata taaataccat caagaccatc aatattaagc
     4561 gagagtctat ctccggctct aacctcacgc tctagttcaa tatcttctac tcgtccagtt
     4621 tcatcgaaag ttactgaata catcatttca aatttccatc taagcgtatg agatgtctaa
     4681 cgaaaatacc ataaagagaa tttgattaca ttgaactttt tcagtgctta cttggcttat
     4741 ctgaagcctt aatcattgcc gcccagtgcg ctatctgatt gcatccattc agcgcctaat
     4801 atgtaaaact attgcaatct atcctgacgc aaatgtggca acaccctgct ccacttatta
     4861 tcctgccaag gctggagttt cacatgtgcc gtttctctgg caaggatttc gcgcgcctta
     4921 tgtagtatct ggggatattc ttgcccgata gaagtaaagc gaccagcttc gcggtgctcc
     4981 gcaacctgaa gaagtggagt aacgttctgg caggcagtta acatcatatc ccctgctcgc
     5041 cataaccaag caagtgtgca aagttcgtta tcagtgaatt gttttgtgat tggggattgt
     5101 tgaatttctc gatcgagaat atccagcacc cagcggcgga attctttggc cacaggagta
     5161 cgagcaaaca tggcgatcag atgggctccg cgaaggctaa atactcgaga ctcctgcatt
     5221 ccacgagggg tggtcacttt gaccaccctt gtcatcatat ctgtaaattc atctgagtga
     5281 cgagagtaaa tacgctgaac tgctttatcg tccgcatatt ccagagctaa accaacttca
     5341 gtggcagtaa gccaaattct gttattgtgg caaatcgggg taaacgtcgt tttgtggaat
     5401 gctaattgag tagtcatagt atcacctcat caggttaacc atcaccacca acgacgccaa
     5461 tcgactggtg gtgaactgtg caaggttggc gtaaccggct actcaaaccc ggcgctcccg
     5521 aagaagccct cacacagccc accataattt gggcgtagcc gtgcttagcg cataaaaaaa
     5581 accgcttaac gcggcatgcg ttgagtagta ttccgggacg ccaatcccgt gtgccgattt
     5641 tgcggcaact cgtaagaata tatccccaga taaatcatgt cgtcaacccc agtaaatgga
     5701 caccacgatg atcatagcca tagtgactac aacagttaca attgcttcag gccaagacat
     5761 aacgatttcc atatcaccat tagccgccag acgaatagat actctcagtt ctggctgttg
     5821 aggtaaaaca ccgaatacaa aaaacacagg tgcatttctg catctgtgac aggaggagag
     5881 agtttaggtt gtatacgcgc ataaatgccc cgtttagcgc agaagtcacc
//
LOCUS       DAZKHK010000210         5899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKHK010000210 DAZKHK010000000
VERSION     DAZKHK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.211"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..781)
                     /locus_tag="ACHOS5_004821"
     CDS             complement(<1..781)
                     /locus_tag="ACHOS5_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779508.1"
                     /translation="MKKTLIALAVAASAAVSGSAMAWTANGTGGSVDMGGTLNPVTKV
                     TPWEAKVGAAVTGLDGQIEKGISKIEIPLTKTIPVLGIRTQSSIPFNGSTNGIDPQID
                     YNGAINTGGFSNNATTLTLDVLDADTSNKIGNLQASFSAGAVANYKTSGGTVSQFVVN
                     APHSGDAFFGGVPNSPSGVNASAVTSLLAELFSDIADNYNSQGIGSWSTSWNINFDNP
                     TSTYSGYYASGIKSGQNLRMTFDSPAQGNDSIVWKATLPVTV"
     gene            complement(1021..1512)
                     /gene="faeF"
                     /locus_tag="ACHOS5_004822"
     CDS             complement(1021..1512)
                     /gene="faeF"
                     /locus_tag="ACHOS5_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HFP9779509.1"
                     /translation="MKKTMMAFALALNALSIQSALAAEYSEKTQYLGVVNGQVSGNSV
                     VKVTRTPTDPVLYRSGSNSPLPAELIIRHAESRPASGGQANITVKQILPDNGEARITL
                     KMALMVDGKRVALSARQQGENVVISVPEAQKLVELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
     gene            complement(1547..2323)
                     /gene="faeE"
                     /locus_tag="ACHOS5_004823"
     CDS             complement(1547..2323)
                     /gene="faeE"
                     /locus_tag="ACHOS5_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HFP9779510.1"
                     /translation="MSKRNAVTTFFTNRVTKALGMSLALMMTCQSAMASLAADQTRYI
                     FRGDKDALTITVTNNDRERTFGGQAWVDNIVEKDTRPTFVVTPSFFKVKPEGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALIEGRKGAEEG
                     LSLQSRPDGRTMLVNTTPYIFAIGSLLDGNGKKIATDNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLNDWGSLQTWTINKKKPTAPEAAKTETADTAEQK"
     gene            complement(2316..4718)
                     /gene="faeD"
                     /locus_tag="ACHOS5_004824"
     CDS             complement(2316..4718)
                     /gene="faeD"
                     /locus_tag="ACHOS5_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HFP9779511.1"
                     /translation="MFFRRAALALAVSAAFASATGIAGEKLDMSFIQGGAGVNPEVWA
                     ALNGSYAPGRYLVDLSLNGKEAGKQILDVTPQDSNELCLTEAWLTKAGIYISADYFRE
                     GYDATRQCYVLTKASSVKVDFDVSTQSLALSIPQKGLVKMPENVDWDYGTSAFRVNYN
                     ANANTGRNNTSAFGSADLKANIGHWVVSSSATASGGDSGDNSTTINMFTATRAIRALS
                     ADLAVGKTSTGDSLLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIANGPSRVTLTQNG
                     RLLHSEMVPAGPFSITDVPLYTSGDVTMKITGEDGREQVQNFPLSVMAGQLSPGQHEF
                     SVAAGLPDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNQDWQGVSAGAVLGLSYLGAV
                     SADGAYATAKYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEEYEDMSSFDPTTL
                     WGREKHGRRTKEEWNTGVSQPVGGLFSLSVSGWQRSYYPASMTGSYRYSDDNGKDTGI
                     TGTLSTQIKGVSLNLGWSGSRNSRGENNWSASASVSVPFTLFDRRYSSSTSVSTSKGG
                     GTGISTGVSGSLNDRFSYGLGGGRDSSGGVSSYLNASYSGDRAYLSGALNQSSSGGTS
                     GSVSASGSVLAVPAAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGNGETDSDGNLVVPL
                     NSYDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVKRYLLQVKQRDGE
                     FVPGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAAKLQDTEKLQEIT
                     CE"
     gene            complement(4775..5317)
                     /locus_tag="ACHOS5_004825"
     CDS             complement(4775..5317)
                     /locus_tag="ACHOS5_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9779512.1"
                     /translation="MKNLSLSVLLLLAGGGVSHATVQKTVFSADVVASACHVVVDADS
                     TGNSGRLTFGTYRKSTGASVPPRDFTVRLYESGATVQGCSAFKAGQVATLDFGNPGQL
                     DAGGVVTRGAGDGIRVDVRAVDTQADYRGRLTQDSHSVNYPVEFAAEGQFRFRAQPVF
                     PANVKAGEYTGALTFVVTYQ"
     gene            complement(5325..5624)
                     /gene="daaA"
                     /locus_tag="ACHOS5_004826"
                     /gene_synonym="afaA"
     CDS             complement(5325..5624)
                     /gene="daaA"
                     /locus_tag="ACHOS5_004826"
                     /gene_synonym="afaA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F1845 fimbrial adhesin operon transcriptional
                     regulator DaaA"
                     /protein_id="HFP9779513.1"
                     /translation="MNSSDLYLSKKHGRTLSPGKMSESQFWLLAEISPVHSEKVLNAL
                     RDFLVLGYTRREACERHEVSQGYFSGALGRIQRTHQAVNRLVPFYISEAGIPYTG"
BASE COUNT         1339 a         1825 c         1329 g         1406 t
ORIGIN      
        1 atacagtaac cggcaatgtt gctttccata caatggaatc attaccttga gccggagaat
       61 caaacgtcat tcttaagttt tgtccagatt tgattccaga agcgtaatag ccactatagg
      121 tagacgttgg gttatcgaaa ttaatgttcc atgatgtact ccatgaacct attccctgag
      181 aattataatt atctgcaata tctgaaaaca actcagccaa gagactggta accgcactcg
      241 cgtttacacc gcttggggaa tttggcaccc caccaaaaaa tgcgtcacca ctatgaggtg
      301 catttacaac aaattgactg accgttcccc ctgaagtttt atagtttgct actgctcctg
      361 cagaaaaaga tgcttgaaga ttaccaattt tattagatgt atccgcatcc agcacatcta
      421 aagtcagcgt tgtagcattg tttgaaaatc cacctgtatt tatggcacca ttgtaatcaa
      481 tttgaggatc aataccattc gtactcccat tgaatgggat actcgattga gtacggatac
      541 ccagaaccgg aatagtcttg gttagaggaa tttcaatctt gctaatacct ttttcaattt
      601 gaccatccag accagtaaca gcagcgccta ctttagcctc ccacggcgtc actttagtta
      661 ccgggttcaa cgtaccgccc atatcaactg aaccacccgt accatttgca gtccacgcca
      721 tggctgagcc ggatactgca gcagaggcag ccacagccag tgcaatcaga gtctttttca
      781 taaaataaat tccttgatat ttcataaata ataaactgaa atcggtaatc cttattttcc
      841 cggaattacc gcaaaaccat cccctgcgga ttcatttaat taaccgcatt tcattaaaca
      901 ctgtgtttaa ctcagaacag ttttacgtta ttcccgttta aaaagacggt aaatcccttt
      961 accagtatga cccgttccgg agtcatggga tgtattgcat ctgttgtcgg agatgaattc
     1021 tcagtcttcc acctgcagcg ctatctgcag atttccccgg taactgaccg ggacttccag
     1081 ctctgccggt gcatccgttc ttaactcaac cagcttctgt gcctcaggta cgcttatcac
     1141 cacattctca ccctgctgtc tggcactgag tgccactctc tttccgtcaa ccatcagagc
     1201 catcttcagg gtgatgcggg cttccccgtt atccggcaga atctgtttca ccgtgatgtt
     1261 tgcctgtccg ccggaagccg ggcggctttc tgcatgccgg attatcagct ctgcaggtaa
     1321 cgggctgtta ctgcctgagc ggtacagcac cgggtctgtc ggtgtacggg tcaccttcac
     1381 cacgctgtta cctgacacct gaccgttcac cacacccaga tactgcgttt tctcgctgta
     1441 ttcagcggcc agtgctgact gaatactgag cgcattcagg gccagggcga atgccatcat
     1501 tgttttcttc atttattacc cctctgttcc gtatgccgga gggcggttat ttctgctctg
     1561 cggtatctgc tgtctcagtt tttgctgctt ccggtgctgt cggtttcttc ttattaattg
     1621 tccaggtctg caggcttccc cagtcattca gggagtccac tttcaccaca tttcccttca
     1681 cctgcacttc atcacccggc atgaacatca gcagtttctg tgctgtctca ttatccgtgg
     1741 caattttctt cccgtttccg tccagcaggc tgccaatcgc aaaaatgtac ggcgtggtgt
     1801 tcaccagcat ggtcctgcca tccgggcggc tctgcaggct gagcccttcc tctgccccct
     1861 tgcggccctc aatcagggcc ttcgggcgat agaacagctt cagcttcgtg cgcagcgcca
     1921 ctgcaatacc gcttccctcc agagccggcg gaatatcctg caggttcagc cagtacaccg
     1981 actctttatc cttcggcaga tggtcagacg ccatgatgat acgcagtgtc tgctgaccct
     2041 ccggcttcac cttgaagaag gacggcgtaa ccacaaaggt cggacgggtg tccttctcca
     2101 cgatattgtc cacccaggcc tgaccaccaa aggtacgctc cctgtcattg ttggtgaccg
     2161 tgatggtcag cgcatccttg tccccgcgaa agatatagcg ggtctggtct gccgccaggg
     2221 aagccatcgc actctgacag gtcatcatca gcgccaggga catccccagt gcttttgtca
     2281 cccggttggt gaaaaacgtc gttactgcgt tacgcttact cacacgtaat ctcctgtagt
     2341 ttctcagtat cctgcagttt cgccgcaggt atccggcact ggcccagggt gatatccccc
     2401 ggcgcatcca ccgtgttaat catcagcaca ccattgttgg ccacaaagcc cagcggtgtg
     2461 ttcttactgt cacgtgccca ggttcccccc ggcacaaact caccgtcacg ctgcttcacc
     2521 tgcagcaggt aacgcttaac tttcagggca tcaaacggca tccacaccac cgccttgtcc
     2581 gttggcacca ccttctgact ggtgttcgtc agttcggtgc tcagcggcag tgtgcccgca
     2641 tcaatcgtca ccgtgttcca gtcatagctg ttcagcggta ccaccaggtt gccatcgctg
     2701 tctgtctctc cgttaccgga cgtcaccttc actccggggg tatccttcac gttcaccacg
     2761 gccacggtat ccccggtcgt gcggctgaac ataatgtcct tcgccgccgg aacggccagt
     2821 acagagccac tcgctgacac agagccactg gtgcctccgg atgatgactg gttcagagcc
     2881 ccactcagat aagcccggtc accgctgtac gacgcgttca gataactgct cacaccgcca
     2941 ctgctgtcac gaccaccgcc caggccatag ctgaaacgat cattcagcga gccggacacg
     3001 ccggtgctga tgcccgtacc accgcctttg ctggtgctca ccgacgtact gctgctgtaa
     3061 cgacggtcaa acagtgtgaa cggtaccgac actgacgccg atgcagacca gttgttttct
     3121 ccccgggagt tccgtgaacc ggaccagccc aggttaagac tgacgccctt aatctgtgtg
     3181 ctcagggtgc cggtaatccc cgtgtcctta ccgttgtcat cgctgtaccg gtaactcccg
     3241 gtcatggatg cggggtaata gctccgctgc cagccggaca ccgacagact gaacagcccg
     3301 cccaccggct gactgacacc ggtattccat tcctccttcg tccggcggcc gtgcttctcc
     3361 cgcccccaca gcgttgtcgg gtcaaaggag gacatgtcct catactcctc actctgccgt
     3421 gaccagctca cccgcagtcc ggtgttcgtc atctcaagtt gtttactcca ggataactgc
     3481 accttatttc cgctgcggct gccatcacgg tatttcgccg tggcataagc cccgtcagca
     3541 gacaccgccc ccaggtaact cagccccaga acagccccgg cgctgacacc ctgccagtcc
     3601 tggttaaaca ccccgccggt gcgcagcgtc agcccgtcca gaccgtaacc gtatgacgcc
     3661 gcaaacacac cacctttcag gtcactgtca tcgtcaggca aaccggctgc cacactgaac
     3721 tcgtgttgcc ccgggcttaa ctgcccggcc atcaccgaca acgggaagtt ctgtacctgc
     3781 tcacgcccgt cttcaccggt gattttcatg gtcacatcac cgctggtgta cagcggcaca
     3841 tccgtgatgg agaacggacc tgccggcacc atctccgaat gcagcaaccg tccgttctgt
     3901 gtcagcgtca ccctcgacgg cccgttcgca atgccgctga acaccggggt ataccccaga
     3961 ttgcccggct tcatgctgtt gttccggctc agcgacacgc cgtacgttcc cgtactgccc
     4021 agcagactgt ccccggtgga tgttttcccg accgccaggt ccgcgctcag tgcgcggatg
     4081 gcccgggtgg ccgtaaacat gtttatcgtg gtggagttat ccccgctgtc accgccactg
     4141 gccgtggcag aagaactcac cacccagtgc ccgatattgg ctttcaggtc cgctgagcca
     4201 aaggccgagg tgttattacg gccggtgttg gcgttcgcgt tatagttcac gcgaaatgca
     4261 ctggtcccgt agtcccagtc cacgttctcc ggcatcttca ccagcccctt ctggggaatg
     4321 gacagcgcca gactctgggt ggaaacatca aaatccacct tcactgacga cgcttttgtc
     4381 agcacataac actgccgtgt ggcgtcatat ccctcacgaa agtaatccgc actgatgtaa
     4441 atcccggcct tcgtcagcca tgcctctgtc agacacagtt cattactgtc ctgtggtgtc
     4501 acatccagta tctgtttccc ggcctccttc ccgttcaggg acaggtcaac cagataacgc
     4561 cccggcgcat agctgccgtt cagggctgcc cagacttccg gattaacccc ggcaccaccc
     4621 tggataaagg acatatccag tttttcacct gcaatacctg tggcactggc aaaagcagct
     4681 gatacggcaa gcgcaagtgc ggcccgacgg aaaaacacgg acgaaaccgg ctctgcgact
     4741 gccttctttc ctgcacacac aatccttttc tccgttactg atacgtgacc acaaaggtca
     4801 gtgcacccgt gtattccccc gccttcacgt tcgccggaaa caccggctgc gcacgaaaac
     4861 gaaactgtcc ttcagcagca aactccaccg ggtaattcac cgaatgactg tcctgagtca
     4921 gacgtccgcg ataatctgcc tgtgtatcca ccgcccgcac atccacgcga ataccatcac
     4981 cggcaccgcg ggtgaccacg ccccctgcat ccagctgtcc cgggttacca aaatccaggg
     5041 tggcgacctg ccctgcctta aaggctgagc agccctgaac cgtggcaccg gactcataca
     5101 gacgcacagt gaagtcacgc ggcggcacag acgcccctgt ggacttgcgg taagtcccga
     5161 atgtcagccg gccactgttg ccggtgctgt ccgcatccac caccacatga caggccgaag
     5221 ccaccacgtc agcactgaat acggttttct gaacagtggc gtgagaaacc ccgccccctg
     5281 ccagaagcag aagtactgaa agcgataaat ttttcatgca actgttatcc cgtatatgga
     5341 ataccggctt ctgatatata aaaaggaacc agcctgttta ccgcctgatg ggtacgctga
     5401 atacgcccga gagcaccaga gaaatatccc tgagaaacct catgtcgttc acaggcctcc
     5461 cttcgggtat aacccagaac cagaaaatcc ctcagcgcat taagaacctt ttcactgtga
     5521 accggagata tttcagctaa taaccagaac tggctctctg acatttttcc gggcgacaaa
     5581 gtacgcccgt gcttcttgct cagatacagg tcactgctgt tcatttacat ctattccaga
     5641 aatataacca gacaaaaaga cgacataaat ccggataaca aaaacgtatt cactctgctg
     5701 tcgtcctgtt ttctgtggca aagactatca cggtgtttta tatcaaaaaa cattaatttc
     5761 acgatcttta tgaccaaaca acaaccattt aatagcacaa aaccccacaa aaccccagat
     5821 aatttaggat aaaaaaccaa aaacaaaaca aaaaacagat aaaaagatcg ttgtttttgt
     5881 aaacaccaat attgcctcc
//
LOCUS       DAZKHK010000211         5867 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKHK010000211 DAZKHK010000000
VERSION     DAZKHK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.212"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..4262
                     /gene="druE"
                     /locus_tag="ACHOS5_004827"
     CDS             <1..4262
                     /gene="druE"
                     /locus_tag="ACHOS5_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruE"
                     /protein_id="HFP9779514.1"
                     /translation="RESRPESQIVTSKEANALAEFDLNSFQCVAEDLESAYAAIESLA
                     ERMGWQKPMIKDHSTLRNWLFDNLTGFGPIETLIEIVSGKAVKLNILSENLFPDSPQQ
                     IAERATDALLALGCYAQRASDGRVLIPTRMHLFYRGLPGLYACIDPDCNQRLGNHSGP
                     TILGRLYTKPLDQCKCASKGRVYELFTHRDCGAAFIRGYVSSEMDFVWHQPNGPLSED
                     EDIDLVPIDILVEETPHVHSDYQDRWLHIATGRLSKQCQDEDSGYRKVFIPDRVKSGS
                     EITFDECPVCMRKTRSAQNEPSKIMDHVTKGEAPFTTLVRTQISHQPASRPIDGKHPN
                     GGKKVLIFSDGRQKAARLARDIPRDIELDLFRQSIALACSKLKDINREPKPTSVLYLA
                     FLSVLSEHDLLIFDGEDSRKVVMARDEFYRDYNSDLAQAFDDNFSPQESPSRYKIALL
                     KLLCSNYYSLSGTTVGFVEPSQLKSKKMWEDVQSKKLNIESKDVHALAVAWIDTLLTE
                     FAFDESIDSTLRIKAAGFYKPTWGSQGRFGKALRKTLIQHPAMGELYVEVLEEIFRTH
                     LTLGKDGVYFLAPNALRLKIDLLHVWKQCNDCTALMPFALEHSTCLACGSNSVKTVEP
                     SESSYINARKGFWRSPVEEVLVSNSRLLNLSVEEHTAQLSHRDRASVHATTELYELRF
                     QDVLINDNDKPIDVLSCTTTMEVGVDIGSLVAVALRNVPPQRENYQQRAGRAGRRGAS
                     VSTVVTYSQNGPHDSYYFLNPERIVAGSPRTPEVKVNNPKIARRHVHSFLVQTFFHEL
                     MEQGIYNPTEKTAILEKALGTTRDFFHGAKDTGLNLDSFNNWVKNRILSTNGDLRTSV
                     AAWLPPVLETGGLSASDWFAKVAEEFLNTLHGLAEIVPQIAALVDEENEDDEQTSGGM
                     KFAQEELLEFLFYHGLLPSYAFPTSLCSFLVEKIVKNIRGSFEVRTVQQPQQSISQAL
                     SEYAPGRLIVIDRKTYRSGGVFSNALKGELNRARKLFNNPKKFIHCDKCSFVRDPHNN
                     QNSENTCPICGGILKVEIMIQPEVFGPENAKELNEDDREQEITYVTAAQYPQPVDPED
                     FKFNNGGAHIVFTHAIDQKLVTVNRGKNEGGSSGFSVCCECGAASVYDSYSPAKGAHE
                     RPYKYIATKETPRLCSGEYKRVFLGHDFRTDLLLLRITVGSPLVTDTSNAIVLRMYED
                     ALYTIAEALRLAASRHKQLDLDPAEFGSGFRILPTIEEDTQALDLFLYDTLSGGAGYA
                     EVAAANLDDILTATLALLEGCECDTSCTDCLNHFHNQHIQSRLDRKLGASLLRYALYG
                     MVPRCASPDIQVEKLSQLRASLELDGFQCLIKGTQEAPMIVSLNDRSVAVGSYPGLID
                     RPDFQHDVYKSKNTNAHIAFNEYLLRSNLPQAHQNIRKLLR"
     gene            4512..5622
                     /locus_tag="ACHOS5_004828"
     CDS             join(4512..4765,4764..5622)
                     /locus_tag="ACHOS5_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543393.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9779515.1"
                     /translation="MRKARFTEHQIIAVIKSVEAGRTVKDVCREAGISEATYYNWKSR
                     YGGMEASDIKKIKDLEDENPRLKQMFADLSLENRALKDVIEKKALKLAFKRELVTHLI
                     TTFGLSIRQACRSLNLSRTVYHYRPDTTPDEPVIVALQAVAERYPRYGFPKLFQVLRR
                     QGYPWNHKRIHRIYCLLKLNFRRKGKQRLLVRNPSPLATPEALNQSWSVDFMHDALVC
                     GRRFRTFNVVDDFNREALSIEIDLNLPALRVVRVLNRIASNRGYPVMLRMDNGPEFIS
                     LALAEWAEKHAVKLEFIQPGKPTQNAFIERFNRTYRTEILDFYLFRTLNEVREITERW
                     VSEYNCERPYESLNNMTPEEYRQHHYLAGISKNAWN"
     gene            complement(5687..>5867)
                     /locus_tag="ACHOS5_004829"
     CDS             complement(5687..>5867)
                     /locus_tag="ACHOS5_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9779516.1"
                     /translation="IEAILELSRKLNIKTIVEGVETMELVNKLMLLGVDYFQGYYFGQ
                     AIELCSFIREYRKTV"
BASE COUNT         1680 a         1194 c         1308 g         1685 t
ORIGIN      
        1 caagggaatc gcgtcctgag tcacaaattg ttaccagtaa agaagctaat gcactggctg
       61 aattcgacct aaattcattt cagtgcgtag ctgaggatct tgaatctgca tatgcagcaa
      121 tagagtctct tgccgaacga atgggctggc aaaagccgat gataaaagat catagtacac
      181 tacgtaattg gttatttgat aatttgactg gttttggtcc tattgaaacg cttattgaaa
      241 tagtttcagg taaagcggtt aagctaaata tcttgagtga aaaccttttt ccagactctc
      301 cacagcaaat tgcagagcga gcgacagatg cattactcgc attgggttgc tatgctcaga
      361 gggcatccga tggcagagtg cttattccaa ctcgcatgca tcttttttat cggggattac
      421 caggtcttta tgcctgtata gatcccgatt gtaatcaacg tttgggtaac catagtgggc
      481 caactatact tggccgcctt tatacgaaac cactggatca atgtaaatgc gcttcaaaag
      541 ggcgagtcta cgaattattt acccaccgtg actgcggtgc ggcttttatt cgtggatacg
      601 ttagttccga aatggacttt gtatggcacc agccgaacgg accattatca gaagatgagg
      661 atatcgatct tgttcccata gatatattgg tcgaggaaac acctcatgta catagtgatt
      721 accaggacag atggctacat atagcaacag gacgcctttc taaacagtgt caagatgagg
      781 attctggtta tcgtaaagtc tttatacctg accgagttaa gtctggatct gaaattacat
      841 ttgatgaatg ccctgtttgt atgcgtaaga caagaagtgc tcagaatgaa ccgtctaaaa
      901 ttatggatca tgttacaaaa ggggaagcac cttttacaac gttagtacgt acacagatat
      961 ctcaccagcc agcgagtcgt cctattgatg gtaaacatcc caatggggga aaaaaagtac
     1021 ttattttttc tgatggccga caaaaagcag ctcggcttgc acgtgatatt cctagagata
     1081 ttgagcttga tttgtttcgg caatccattg ctctcgcctg ttctaaactg aaagatatca
     1141 atcgggaacc caaaccaaca tcagtacttt accttgcttt cctatcagtc ctttctgaac
     1201 atgacttgct tatttttgat ggggaagatt cacgaaaagt tgtaatggcc cgtgatgaat
     1261 tttatcgtga ttataatagc gatctggctc aagcttttga tgataacttc agtccccaag
     1321 agtcaccgtc acgatataaa atagcgttgc ttaaactttt atgtagcaat tactattctc
     1381 tttccggaac aacagttggt tttgttgaac catcgcagct taaatcaaaa aaaatgtggg
     1441 aagatgtgca gtccaagaag ctaaatattg agagcaagga tgttcatgct ttagctgttg
     1501 cttggattga taccttactc actgaatttg cttttgatga atctattgat tcgacactac
     1561 gaatcaaagc cgctggattc tacaaaccca cttggggtag tcaaggacgg tttggaaaag
     1621 ctcttaggaa aaccctgata cagcatcctg ctatggggga gctttatgtg gaagttttgg
     1681 aggagatttt tcgtactcat ctgacattag gaaaagatgg tgtctacttt cttgctccaa
     1741 atgcactacg tctgaaaata gatctcttgc atgtctggaa acaatgtaat gactgcacgg
     1801 cactaatgcc atttgcttta gaacattcta cttgccttgc ttgtggtagt aacagtgtca
     1861 aaacagtcga gccgtcggaa agcagctata ttaatgcacg aaaaggattc tggcgttcgc
     1921 cggtagaaga agttttggtt tcaaattcgc ggcttctaaa ccttagcgtt gaagagcata
     1981 ctgctcaact ctcacataga gatagggcca gcgttcatgc cactacagaa ctctacgaac
     2041 tgagattcca agatgttctt attaatgata acgacaagcc cattgatgta cttagttgta
     2101 cgacgacgat ggaagtgggg gttgatattg gatctctggt tgctgttgct ttaagaaacg
     2161 tccctccgca acgagaaaat tatcagcaac gtgctgggcg agcaggccgc cgtggcgcat
     2221 ctgtttcaac ggtggttaca tattctcaaa atggccctca tgatagttat tatttcctta
     2281 atcctgaacg cattgttgca ggttctcctc gtacacctga agtgaaagta aataatccca
     2341 aaatagccag aagacacgtt cattcttttt tagttcagac cttttttcac gagttaatgg
     2401 aacaaggaat ttataatccc acagagaaaa ctgccatact tgagaaagca cttggtacta
     2461 cacgagattt ttttcatgga gcaaaagata ctggcctaaa tctcgatagc tttaataatt
     2521 gggttaaaaa ccgtattcta tctactaatg gtgatttgag aacaagtgtt gcagcatggc
     2581 ttcctcctgt tcttgaaact ggagggcttt ctgccagtga ctggtttgct aaggtagcag
     2641 aggaattttt aaatacactc catgggctgg ctgaaattgt tccacaaatt gccgctcttg
     2701 ttgatgagga aaatgaagat gatgagcaga cttctggtgg aatgaaattt gcacaagaag
     2761 aattacttga gttcctgttt taccatggtt tattaccaag ttatgcattt cctacaagcc
     2821 tctgtagttt cttggtagaa aaaattgtaa agaatattag aggttctttt gaggtgcgaa
     2881 cagtacaaca gcctcagcaa tcaatttctc aggctctgag tgaatatgcc ccgggacgtt
     2941 tgattgttat tgataggaaa acctatcgct ctggtggtgt tttttctaat gcattgaaag
     3001 gcgaactaaa ccgggcaaga aagcttttca ataatcccaa aaagtttatt cattgcgata
     3061 agtgctcttt tgtccgcgat cctcataata atcagaatag cgaaaatact tgtccgatct
     3121 gtggtggcat tctaaaagta gaaataatga ttcagcccga agtctttgga cctgaaaatg
     3181 ccaaggaact taatgaggac gacagagagc aagaaatcac ctatgtaact gcggcacaat
     3241 atccacaacc tgttgatcct gaagatttta agttcaataa tggaggtgct catattgttt
     3301 ttactcacgc aatagatcag aaactggtga cggtgaaccg agggaaaaat gagggggggt
     3361 ccagtggttt ttcagtatgt tgcgaatgtg gtgcggcctc cgtttatgat tcctactcac
     3421 cggcaaaggg ggcacatgaa agaccgtata aatatatagc aactaaggaa acgcctcgct
     3481 tatgctctgg cgagtataaa cgcgtttttc tcggacatga tttccgtact gatttgcttt
     3541 tattacgaat aaccgttggg tctccgcttg taactgatac ttcaaatgct atcgttttac
     3601 ggatgtatga agatgcatta tatacaatag cggaagcact aaggcttgca gctagtcgcc
     3661 ataaacaact ggatcttgat cctgctgagt ttggctctgg tttcagaatt ttacccacta
     3721 tagaggaaga tactcaggca ttggatctct tcctttatga tactttatct ggcggtgcgg
     3781 gttatgcgga agtagcagca gcgaatctag atgacattct tactgcaaca ctcgcattgt
     3841 tagaaggttg tgagtgcgat acctcctgta cagattgtct caatcatttc cacaaccagc
     3901 atatacaaag ccgtctcgat aggaaactag gtgcatcttt acttcgttat gctctatacg
     3961 gaatggttcc tcgttgtgct tcacctgata ttcaggtaga aaaattgtct caattgaggg
     4021 caagtctgga attggatggt tttcaatgcc taattaaggg aactcaggag gcacctatga
     4081 ttgtgagttt gaatgaccgt tctgttgcag tgggaagtta ccctggtctt attgatcgac
     4141 ccgactttca acacgacgta tataagtcaa agaatactaa tgctcatata gcctttaatg
     4201 aatatcttct tcgttcaaat ctgccacaag cgcatcaaaa tattagaaaa ttgttgcgct
     4261 gatagcagta ttgagtgccc taaagtccta tagggcactc aaggttattc tcttttaata
     4321 tttgttaata gcatgtattt taaggctttc agtccttgag ctggctttta cacaacgaga
     4381 aagaggtaat tataatacca gccggatatg gcgagaaatg gtggcccggg ggtgtaagta
     4441 tcccgcataa tcgtgccatt catatttaga gatcatccgg cataatcaat ctgctaacga
     4501 aggagatcgc tatgcgtaaa gcccgtttta ctgagcatca gatcatcgct gtgattaagt
     4561 cagttgaagc cggacgaacc gttaaagatg tctgccggga ggccggtatt tctgaagcca
     4621 cctactacaa ctggaagtcc agatacggtg gcatggaggc ttctgatatt aaaaagatca
     4681 aggatcttga ggacgagaac ccacgtctca aacagatgtt tgccgacctg agtcttgaga
     4741 acagggcgct gaaagacgtt atcgaaaaaa gctttaaaac tagcctttaa gcgtgagctg
     4801 gttactcatc tgataacgac gttcggactc agtatccgtc aggcctgccg gagtctgaac
     4861 ctgagcagaa cggtttacca ttaccgcccg gataccacgc ctgacgagcc cgttattgtc
     4921 gctttgcagg cagtggcaga gcgataccca cgatacggtt ttccgaaact tttccaggtt
     4981 ttgcggcggc agggataccc gtggaatcac aaaaggatcc atcgtattta ttgtctgctg
     5041 aagctgaact ttcgccgtaa gggcaaacag cggttgctgg tgcgtaatcc ctcgccactg
     5101 gccacgccgg aagcgctgaa ccagagctgg tctgtcgatt ttatgcatga tgccctagtc
     5161 tgtggccgtc gttttcgcac gtttaatgtc gttgatgact ttaaccgtga ggcgttgtcg
     5221 attgaaatcg atctgaatct gccagctctg cgagtggttc gtgtactcaa caggatcgcg
     5281 tcaaaccgtg gctatccggt catgctacgt atggataatg gtccggaatt tatctcactg
     5341 gcactggctg aatgggcaga gaaacatgca gtaaaactgg agtttatcca gccgggtaag
     5401 ccgacgcaga acgctttcat cgagcgtttt aacaggacat accgtacaga aatactcgat
     5461 ttttatctgt tcagaacact gaatgaagtg cgggaaatca ctgaaagatg ggtgtcagaa
     5521 tataactgtg aacgcccgta tgaatcacta aacaatatga cgccagagga atatcgtcag
     5581 caccattatt tggccgggat ctcaaaaaat gcatggaact aaaacgggaa tgtgtactcg
     5641 agcattttta agaaaagtca cttacacaga ttagcaaaca agatcactat acagtctttc
     5701 tatattctct aataaacgaa catagctcaa tagcctgacc aaagtaataa ccttgaaaat
     5761 aatcaacccc cagcaacatt aatttattta ctaactccat ggtttcaact ccttccacga
     5821 tagtttttat atttaacttc ctagataatt caagaattgc ttctata
//
LOCUS       DAZKHK010000212         5795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKHK010000212 DAZKHK010000000
VERSION     DAZKHK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.213"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="ACHOS5_004830"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="ACHOS5_004830"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9779517.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHOS5_004831"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHOS5_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP9779518.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /gene="ydbJ"
                     /locus_tag="ACHOS5_004832"
     CDS             complement(1535..1801)
                     /gene="ydbJ"
                     /locus_tag="ACHOS5_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP9779519.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHOS5_004833"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHOS5_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9779520.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAASVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGIWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1451 a         1481 c         1498 g         1365 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcgaaag aactggggct caaagtagtc
      301 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcaa tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cggcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc ggcaattgaa gcgctgaaaa atcagaaaat tggttcgttg
      781 ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg aagataaatc caacgacgtg
      841 atccaggatg acgtattccg tcgcttgtct gcctgccaca acgtgttgtt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcattccagg ggagcttatt cagataatcc ccaaagacct ttcatcctct attcttaaaa
     1081 tagtcctgaa tcagaaacag taattgagaa ccacaatgaa gaaagtagcc gcgcttgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtagtgacaa aattgctgta acgccagaac
     1201 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa agatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac cgccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg gcgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tcttttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga acgattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcatg ggccggaaac ggcttgaaaa acgtttgggg
     2221 agacacacca cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 tgctttgcaa actggtgccc tctcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccaacg ctgtacaaac tggcaggcga actgacgccg tttgtcctgc atgttgcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg ttatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc agcaagcgtc caggaagcgc aagactttgc
     2521 tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcaca tctcacgaaa tcaataaaat tgtcccgctg gccgatgaca cgattcttga
     2641 actgatgccg caggcagaaa ttgatgctca tcgcgcccgg gcactcaacc cggaacatcc
     2701 ggtgatccgc ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccatggtac aacgcagtct atgaccatgt tgaacaggcg atgaatgatt tcgctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgagta ttacgggcat ccgcaagcgg aacgggtgat
     2881 tatcctgatg ggctcagcca ttggcacatg tgaagaagtg gttgatgaat tgctgacccg
     2941 tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctccg ccaaacactt
     3001 gctgcaagct ctgccgggat ccgtgcgcaa cgtggcggta ctggacagaa ccaaagagcc
     3061 tggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag aagcctttaa
     3121 taatggcgag cgtgaaaccc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtcactgc cgttgccgga
     3301 aaacacccta ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcgga aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctt aacacgccgt acagcgcagc tgaagtgtgg tcgcgcttgc cgcaggaagt
     3661 tcaggccgtg ctaaaccaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcagatt ctgcctggcg atagcgccct ggcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcacg
     3901 tgaatccgta gaagaagttc cactgcagcc agttaatccg catagcgcca atcgaccgcc
     3961 agtggtttcc gatgccgctc ctgatttcgt gaaaaccgta accgctgcga tgctcgccgg
     4021 gcttggcgac gccctccccg tttcggcgct gccgccagac ggcatctggc cgatgggcac
     4081 cacgcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgtg tcgccgcttg cccacactca gctattcgcg caaaagtggt
     4201 accgcctgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggctccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccggaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt ccggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attgctgact cagctctatg gcgaccggat gttgatcgct aatgccactg gctgttcttc
     4621 aatatacggc ggtaacctgc cttcaacacc atataccacc gatgccaatg gtcgtggacc
     4681 ggcatgggcg aactcgctgt ttgaagataa cgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctcgat caatttgccg ataaaatccc
     4801 ggcggaatta ctgactgcgt tgaaatcaga cgccacgcca gaggttcgtc gtgaacaggt
     4861 tgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggt gatggctggg cttacgatat
     4981 tggctttggc ggtctggatc atgtattgag cctgacggaa aacgtcaata ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     5101 agtaactaag tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctt ggcgcacagc tcaaccagac
     5221 agtgaaagcg attcaggaag cggaagcgta tccggggcca tcgctgatca tcgcttatag
     5281 cccatgtgaa gagcatggtt acgatctggc actcagccac gatcagatgc gccaactcac
     5341 tgctaccggc ttctggccgt tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga
     5461 gcagcgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattatc caattctaaa aaaaagcccg
     5641 gacgactgtt cgggcttgtc ttcttatgtg ttggaaaatc agtagcaatg caatggccca
     5701 acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa
     5761 attaaaagta taatttttat tttgtatgtg ttatc
//
LOCUS       DAZKHK010000213         5781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKHK010000213 DAZKHK010000000
VERSION     DAZKHK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.214"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..282
                     /locus_tag="ACHOS5_004834"
     CDS             <1..282
                     /locus_tag="ACHOS5_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016265937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFP9779521.1"
                     /translation="HIQRNTLTHYRFNVKPAINNISNEHFWLLIELSGIRSDKIIMSL
                     REHLVDGFTKREVCERNDVSLSYFSISLKKISHIHNVCALLSEYYNNSF"
     gene            335..1582
                     /locus_tag="ACHOS5_004835"
     CDS             335..1582
                     /locus_tag="ACHOS5_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP9779522.1"
                     /translation="MKRKHLLLLLLFSFSTNSAPLYSLIREAVMHDPIVMEARAELTS
                     AQSRIEQASSAHWPVVTATGSKLLSQSHRYSYDYDTEDILPGIRGEVNIFASGAIEAD
                     VRRSESEAEYYHYKMEETKEETIHSFVSLYLDALREKQSIAVLEQSLSRHNAILNDLN
                     TISIHDTGRESELVQAEARRLMVRQQINSRSRVLKTTLGKLSTWTKNPVTEADLENPF
                     SRMTEAKLLTDFTQAPQKGNPSWLASQADVESKKAALKAQELARYPRVDLTGSVTRDD
                     QQIGVNLSWDLFNRNASYGVTEKAAQIVAATGRLDSVARMIDETGRLSLITVRQSRGE
                     METLRRQEQASARVVDFYRLQFQVARKTLIELLNAENELYSVGLSRVQTEDQMLHGML
                     DYLYSQGMLLKWSGVNLSGEEEK"
     gene            1607..3754
                     /locus_tag="ACHOS5_004836"
     CDS             1607..3754
                     /locus_tag="ACHOS5_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFP9779523.1"
                     /translation="MKSIFEGLSQITALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRSPEDIPSLAVPALLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDALMAVYSGYCWFVQPQAKQDRRSELPEYDMSGAWFWRVLWRFRAYYGQVIVA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVITAILFECAAKLIRSYLTDV
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFISVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIISFSTAIQQINTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNEI
                     VRRPVERESQTHYLTVKDIQGEFHFSAVTFFYKPELPAALKGMSVCIRPGEKVGIIGT
                     IGCGKSTLLKMMVGLYRQNKGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPCGLDMQIGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTENIVLKGLNHWSRGRTMVVVTHRLQVLNIVDRIIV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3815..5062
                     /locus_tag="ACHOS5_004837"
     CDS             3815..5062
                     /locus_tag="ACHOS5_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP9779524.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDDVPADLRERESTV
                     YRLRKTELAQSIAGLKQSKALLDKEIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRIRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKILPVIPGMIASVDIKT
                     GQKSVFQYLIKPITRMKQALQER"
     gene            5261..>5781
                     /locus_tag="ACHOS5_004838"
     CDS             5261..>5781
                     /locus_tag="ACHOS5_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779525.1"
                     /translation="MKMIFTGKVSGEKTVLTVGGRHTVKAQPGEQYGLIDEVTGLVPD
                     GVEADRSGDDLILRKKEEDTEVRIEGFWEECQPGETQCTAVFNIVGEDGQVTEAVLTQ
                     DGPVLDDITAGQSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGSKHRHENDDSD
                     TTAPSSPALKAED"
BASE COUNT         1575 a         1140 c         1546 g         1520 t
ORIGIN      
        1 catatacagc ggaatacgct aactcattat cgatttaatg taaaaccagc tataaataac
       61 atttcaaatg aacacttctg gttactaata gaattatctg ggattcgaag tgataagatc
      121 attatgtcat taagagagca cttagtagat ggttttacaa aacgagaagt atgtgaaaga
      181 aacgatgttt cactgagtta tttttctatt agcttaaaga aaatatctca tatacataat
      241 gtctgcgcat tgctaagtga atattataat aatagttttt agtgcaaaag cacggacaat
      301 cacgttatta atattttaaa taaaaatcat aaaaatgaag cgtaaacatt tgttattatt
      361 attgttgttt tcattttcca ctaacagtgc gcctctttac tccttaatta gggaggcagt
      421 tatgcacgat cccatagtaa tggaagcccg ggcggagtta acttcggcac aatcccgcat
      481 agagcaggca agctctgcac attggccagt tgtcacagct acaggaagta aactcctttc
      541 acaaagtcac cgttattcct acgattatga cactgaagat attttacccg gtattcgtgg
      601 tgaagtgaat atatttgctt caggggctat tgaggcggat gtgcgtcgga gtgagtcaga
      661 agccgaatat tatcattata aaatggaaga aacaaaagag gaaacaattc actcttttgt
      721 ttcattatat cttgatgcac tcagggaaaa acaatccatt gcggtacttg aacagagcct
      781 ttcccggcat aacgcaattc ttaatgacct gaataccatc agtattcatg ataccgggcg
      841 ggagtctgag cttgttcagg ccgaagccag aaggttgatg gttcggcagc agataaattc
      901 taggagcaga gtacttaaaa ccacgctggg aaaactgtcc acttggacaa aaaatccggt
      961 aaccgaagct gatcttgaaa atcctttttc taggatgaca gaggccaaat tattaactga
     1021 ttttacacag gctccacaga aaggtaaccc gtcgtggctt gccagccaag ctgatgttga
     1081 gagtaaaaaa gcggcactga aagcacagga gcttgcccgg taccctcggg tggatttaac
     1141 ggggtctgta acccgggatg accagcagat aggtgtcaat ctgtcttggg acctctttaa
     1201 ccgtaatgcc agttatggtg ttacagaaaa agctgcgcaa atagtggcag ctaccggacg
     1261 actggactct gtcgcccgaa tgattgatga aaccgggcga ttatctctga taacagtcag
     1321 acaaagtcga ggggaaatgg aaacgctcag acgtcaggaa caggcttcag ccagagttgt
     1381 ggacttttat cgtcttcagt ttcaggtggc aagaaaaaca ctgattgaat tactgaatgc
     1441 tgaaaacgaa ctgtacagtg tcggactctc ccgggttcag acggaggatc agatgctcca
     1501 cggtatgctg gattatctgt attcccaggg aatgctcctg aaatggagcg gagtgaatct
     1561 ttctggtgaa gaagaaaaat aacggggttg aagggttggt aattaagtga agagtatttt
     1621 tgaaggatta tcacagataa cggcgttact gggtacacct gtatctgctg agactctggc
     1681 tgccggtact gtccgtacag atgtatccgg gattgatttt cgttctgttg gcgagtttct
     1741 tcgtagtgaa ggatttgata atcatctttc ccggcgatct ccggaagata tcccgtcact
     1801 tgcggttccg gcgcttcttt tattgaacgc tcaggaagct attgtggtag tgcgtattga
     1861 gggggccggt ctggcaagac agtatcatgt gcttcagcct ggaggtctcc gtaagacact
     1921 ttctcacgat gcattaatgg ccgtttattc tggttattgt tggtttgttc agccacaggc
     1981 aaagcaggat cgtcgctcgg agctgccaga gtatgacatg agcggggcat ggttctggcg
     2041 ggtactgtgg cgattcaggg catattacgg acaggttatt gttgcgagtt ttatcattaa
     2101 ctttcttgct ctggtcagtt ccctgtatgt aatgaacgtc tatgaccggg ttatacctaa
     2161 tcgttcctgg gaaacgttat ggatgctgag tatcggggtg attacagcaa ttctgtttga
     2221 gtgtgctgca aagttaatcc ggagttatct gacggatgtt gcgggtaaga aggcagacct
     2281 gattatcagc tctgctctgc tacgtcgggt gatggcggta aaaatgtctg acaggccggc
     2341 atcctctggt tcatacgcca gcaatttgcg ggaatatgag tctgtcaggg agtttatgac
     2401 cagcgctacg ttactggctt ttgttgatct gccttttatt ctgcttttca tcagtgttat
     2461 cgggctcgtg ggaggctggc ttgcggttgt acctcttacg ctggtcccgc tggttattct
     2521 ggcaggaata ttactgcagc gaccgctttc ccgtcatatt aatgcctcca tgcgggaatc
     2581 atcgcagcgt cagggattac ttgtggaggc tctggacgga atagaaaccc tgaagctgaa
     2641 taatgctgtt aactgggttc agcgacgctg ggatggatat actgcaagtg tcgcccgttc
     2701 gtccattcgg gtaaaaaata ccagtaatgt cataatcagc ttttccacag caattcagca
     2761 aattaatact gttctgctgg tcattctggg aacgtacctt attcacagtg atgacccaca
     2821 taaccgtatc accatggggg cactgattgc agccgttatt ctttccggcc gcgcgcttgc
     2881 accgcttggc cagatagcat cactggccac ccgtttccag caggcccgtc tggctatgca
     2941 gggggtgaat gaaatagttc gccgcccggt ggaacgggaa agtcagacac attatctgac
     3001 cgtgaaggat atccaggggg aattccattt cagcgcagtc acgtttttct ataagcctga
     3061 attacctgca gccttgaaag gtatgtctgt ctgtatccgg cccggagaaa aggtgggcat
     3121 tattggcact atcggatgtg gtaaatcgac gctactgaaa atgatggtag ggctataccg
     3181 tcaaaataaa ggacaaatta cccttgacgg ggttgctatg cagcaactgg atccctgttt
     3241 tgttcgggat aaggttttcc tgcttgagca gaatccccgg ctttttctgg gaactctgcg
     3301 tgaaaatctg cagctttcgc gtcttccggg aagcctgtcg gatgtggata tgattaatgc
     3361 agccggacgt ttcgggtttg accgttttat tcgtcatcac ccatgcgggc ttgatatgca
     3421 gataggggaa ggaggtcagg ggctgtcagg aggacagcgt cagatggtcg ctctgacgag
     3481 aatggtactg ggttctcccc gggtagttct gcttgatgaa cccacgacgg gtcttgatct
     3541 gcagacagag aacattgttc tgaaagggct gaatcactgg agccgtggac gcacaatggt
     3601 ggtggtgacg catcgtctgc aggtgctgaa tattgttgac cgtattatcg tgatgaatga
     3661 aggccgggta atgatggatg gtccccgcga tgaaatactg aaaaaactgt cagggaaaca
     3721 gcagagtggg gagagcagag cgataacttc atgagcgtca agcggataag aaataaagag
     3781 catatatgtt taacagtttg tggaccgcag gggtatgaca gaaaaaacgg aacggaataa
     3841 agcgtcgtca gaggtggtta ggcagtcaga cctggccctg ttgacagatg tacaggcagc
     3901 ccttcagcag gaaaagcatc atgccctttt cctgatggtg atttttctgc tggtactggt
     3961 agtgacgttt gttatctggg catggaacag tcctctggat gaagtgaccc gtggtcaggg
     4021 gagtattatt cccggcagca gggagcaggt tatacagacc cttgacccgg gtattttgaa
     4081 aacacttgag gtcagggaag gagatattgt tgagaaaggt caggtactgc tgacactgga
     4141 tgatacccga agctctgcca tgctgaggga aagtgaagcc cgggtgaata acctggaagc
     4201 agtcagggca agactccgtg cagaagctta ttccgaatca ctgacatttc cggatgacgt
     4261 gcctgcagat ttgagggaga gggaaagtac tgtttatcga ctacgaaaga ctgaactggc
     4321 acaaagcatc gccggcctta agcagagtaa ggctctgctg gataaggaaa tagcaatgac
     4381 ccggccaatt gtgcgtgaag gggcgatgtc ggaagtcgaa ctgttacgta tgcagcgtca
     4441 gtcagcggag ctgcagttac agatggatga gaaacagaat aaatacctga cggaagctgg
     4501 agctgaacta gtgaagacag aagcagagct tgcacaggca aaagagaata tggctggcag
     4561 agccgacccg gttgagcgtt cacgtatcag ggctcccctg agaggcatcg taaaaaatat
     4621 tcgggtaaat actttgggag gcgtggtttc tgcaggacag gacataatgg aaattattcc
     4681 gctggaagac cagttgctga ttgaggctta tataaatcca cgggatgttg cctatgtcag
     4741 aacagggatg ccggcacttg taaaactgac ggcttatgac tatgccattt atggtggtct
     4801 tgacggagtg gtgacactgg taagtccgga tactttacgt gaccagaaac ggccgggaga
     4861 tttaaaactg gatccaaatg aagcatatta tcgagtactg gttactacca gtaataatta
     4921 tctgacagac aggaacggaa aaatattgcc agttattcct ggcatgatag ccagcgtgga
     4981 tataaaaaca ggtcagaaaa gtgtatttca gtacctgatt aaaccaatca ccagaatgaa
     5041 acaagctctt caggaacgat gatttattat attattaata tcatggctcc gtaatacaga
     5101 cactcaacag gaagtctgta tcgtatcgca atgaaaaaat aattttatta aatttaagga
     5161 gaataagtca cgtatagaaa ttattatttt tccacctctg gaaatgatta atttatttta
     5221 tattgataat cgcaggtttt aattagccta aggattttat atgaaaatga tttttaccgg
     5281 taaggtcagc ggagaaaaaa ccgtgctgac tgtcggaggt cgtcatacag ttaaagcaca
     5341 gcccggggag cagtatggac tgatcgatga ggtgaccggc ctagtgcctg acggtgttga
     5401 ggcggatcgc tccggtgatg atttaattct gcgtaaaaag gaagaagata ctgaggtccg
     5461 gatagaggga ttctgggaag aatgccagcc cggggaaaca cagtgtacgg cggtatttaa
     5521 tattgttggg gaagatggac aggtcaccga agccgtactg actcaggatg gcccggtgct
     5581 ggatgatatt actgccggac aatcgggaac cttgtcagat gatgatcgtg gcggttttat
     5641 atggttgggc ggtctggcat ttggtggggg actggctgct atggctttag ctgcaggtgg
     5701 cggtggaagc aaacatagac atgaaaatga tgactctgat actacagccc catcgtcacc
     5761 agcactgaag gcagaggatg a
//
LOCUS       DAZKHK010000214         5656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKHK010000214 DAZKHK010000000
VERSION     DAZKHK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.215"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..341
                     /locus_tag="ACHOS5_004839"
     CDS             <1..341
                     /locus_tag="ACHOS5_004839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779526.1"
                     /translation="AKFNICSTSRLTYDIISQLHLPQNGYFMEHSNFLVAIIMLMLRS
                     LAIIPAWFVSLRHEIVTFLFQTVYFLEIWGNAGDAFSSTFVGKYGFGHCDLNLPQSQL
                     FAGRYGKQPI"
     gene            1139..1855
                     /gene="nanC"
                     /locus_tag="ACHOS5_004840"
     CDS             1139..1855
                     /gene="nanC"
                     /locus_tag="ACHOS5_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP9779527.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1875..2981
                     /gene="nanM"
                     /locus_tag="ACHOS5_004841"
     CDS             1875..2981
                     /gene="nanM"
                     /locus_tag="ACHOS5_004841"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP9779528.1"
                     /translation="MNKTITALTIIMASFATNASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTTIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            3046..4026
                     /gene="nanS"
                     /locus_tag="ACHOS5_004842"
     CDS             3046..4026
                     /gene="nanS"
                     /locus_tag="ACHOS5_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9779529.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDKEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSEPHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVDAFRRALKQYHSQLNKIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNVPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISNRFVEAILQFWRER"
     gene            complement(4034..4138)
                     /locus_tag="ACHOS5_004843"
                     /pseudo
     CDS             complement(4034..4138)
                     /locus_tag="ACHOS5_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4238..5110
                     /locus_tag="ACHOS5_004844"
     CDS             4238..5110
                     /locus_tag="ACHOS5_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP9779530.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLRNAELCSTAGSSRQP
                     PVKELTEHRVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYKYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTITLPQVRKNYPF
                     EGFVEWHRKEVFRG"
     gene            5155..5334
                     /locus_tag="ACHOS5_004845"
                     /pseudo
     CDS             5155..5334
                     /locus_tag="ACHOS5_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT         1675 a         1204 c         1260 g         1517 t
ORIGIN      
        1 cagcaaaatt taatatttgt tcaactagcc gtctcacata tgacattatt agtcaactgc
       61 atttgccaca gaatggctat ttcatggaac acagtaattt cttagtggct attatcatgc
      121 taatgcttag gtccctagcg attattcctg cctggttcgt cagcttacgc catgagattg
      181 ttacattcct ttttcagact gtgtatttcc tggagatatg ggggaacgca ggagacgctt
      241 tttcatctac attcgttggt aaatatggct ttggtcactg cgacttaaac cttcctcaat
      301 cacagctatt cgccgggcga tacggcaaac aacctatctg aaacatcatc ctgaaaacac
      361 caacatctac aagcctttcc agatcgactt caggggtgac cacttacaag ccaaaaacca
      421 gaaaacatat ttcatgatga gaattatgat cagcctgatg gcgggtaaac acttattttc
      481 accacgctca ctgagttagc ttgcaaagct tcccttgttt agaccataaa taaattattt
      541 ataaaacaaa tagataaaag aaacccttta gtttttgaag accaccgcaa gtgttcgtct
      601 ggcttcacat ggcatcttcc tcttagaaaa agatcgacat attttgtgac acgaattaca
      661 aatctcgttt tgttgtatgg attgcgtgat ttttgatctg gtataacagg tacaaaggtg
      721 caccacgata gtcaatgaga cagggcatct cgcaatctat ggcaaacatc acttcagttc
      781 tttctcatcg ggtgatgaaa acgcacttca gtctgaaagg aatatgaaaa tgagatcaac
      841 agacactcta ttttatgact ctgggtaaaa tggattgagt aagtgatata gcttacgaat
      901 attcaaatca attaaacatc agaagagatt ttatactcag gtatttaatc tggttctctg
      961 tttatttaaa taatgtggaa agagaatttt cacaggagac cttatacaaa aaatataaaa
     1021 aacagctact cgttgccaaa gacactataa gcctggcaaa aaatattaca caacataaat
     1081 gctaattgtt tatgcgggct ttgtattgct ttctgtatcc tacaaatgag tgaaatttat
     1141 gaaaaaggct aaaatacttt ctggcgtatt attactgtgc ttttcgtccc cattaatttc
     1201 tcaggctgcg acactggacg tacgtggtgg atatcgtagt ggaagccacg cctatgagac
     1261 tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg gcaagtatgg aaagtaatac
     1321 ctggaatacc attcatgata ataaaaagga aaatgccgca ctcaatgatg ttcaggttga
     1381 agttaattac gcgattaaac ttgatgatca atggactgtg cgcccgggaa tgttaacgca
     1441 ttttagcagc aacggtacac gctacggacc ctacgtaaaa ctgtcctggg acgcgacaaa
     1501 agatcttaat tttggcattc gctatcgtta cgactggaaa gcttaccgac aacaagactt
     1561 atccggtgat atgtctcgtg ataacgttca tcgttgggat ggatatgtca cttaccatat
     1621 taatagtgat ttcaccttcg catggcaaac gacgctatac agcaaacaga acgattatcg
     1681 ctatgcaaac cataagaaat gggcgacgga aaatgcattt gttctacaat accatatgac
     1741 gcccgatatt acgccataca tagaatatga ctaccttgac cgtcagggtg tttacaacgg
     1801 cagagataat ttatcggaaa acagttatcg cattggtgtg tcatttaaac tgtagtagac
     1861 aggagacagt cacaatgaat aaaacaataa cggcgcttac tatcataatg gcttcatttg
     1921 ccacaaacgc gtctgtatta ccggaaactc ctgtgccatt taaaagtggt accggagcaa
     1981 ttgataacga cactgtctac attgggttag gtagcgcagg tacggcatgg tacaagctgg
     2041 atacacaggc caaagataaa aaatggacgg cgttagctgc attccctggc ggaccgagag
     2101 atcaagcaac ctctgcattt attgatggca atctgtatgt gtttggcggc attggcaaaa
     2161 acagcgaggg cttgactcag gtatttaatg acgtacacaa atacaacccc aaaaccaata
     2221 gctgggttaa attgatgtcg cacgcgccga tgggcatggc gggccatgtg acttttgtac
     2281 acaacggcaa ggcttatgtt actggcggtg ttaaccagaa tatcttcaat ggctattttg
     2341 aagatctcaa cgaagctgga aaagattcaa ccactataga taaaatcaac gcccactatt
     2401 ttgacaaaaa agcagaagat tatttcttca ataagtttct gttatctttt gatccctcta
     2461 cacagcaatg gagttacgct ggcgaatctc cctggtacgg aacggctggt gcggcggttg
     2521 tgaataaagg tgataaaacc tggcttatta atggcgaagc caaaccagga ttgcgaacgg
     2581 atgccgtatt tgaacttgat ttcaccggta ataatttaaa atggaataag cttgctcccg
     2641 tcgcatcacc agatggcgtc gctggcggtt ttgcaggcat gagcaatgac tcacttatat
     2701 ttgccggagg ggccggattc aaaggttcac gagaaaatta ccagaacggt aagaactatg
     2761 cgcatgaagg cctgaaaaaa tcatatagcg ctgatattca tctttggcat aacgggaaat
     2821 gggataaatc gggcgaatta tcgcaaggtc gggcctacgg agtatcattg ccctggaata
     2881 atagtttatt aattattggc ggtgaaactg caggcggcaa agcggtgacg gattcagttt
     2941 tgatttctgt gaaggataat aaagtcacag tacaaaacta acgcttcagg gccccggtaa
     3001 aggggccctg ctatcaattt gtcatttgta ttacgcgaaa attatatgaa cgcaataata
     3061 tcgcccgatt attactatgt tcttaccgtt gctggtcagt ctaatgccat ggcgtatggc
     3121 gaaggactac cattaccgga caaggaagat gcgcctcatc ccagaattaa acaattagcg
     3181 agatttgcgc atacacatcc cggaggcccg tcatgtcact ttaacgacat tattccactg
     3241 actcactgcc cacacgatgt tcaggatatg cagggttatc accatcctct ggcaacgaat
     3301 catcaaacac agtacggcac cgttggccag gcactgcata tagcacggaa attactgccc
     3361 tttattcctg ataatgcagg ggttctcatc gttccgtgtt gccgtggcgg atcagctttt
     3421 accgcgggca gcgaagggac atattcagaa ccgcacggag ccagccatga tgcttgtcgt
     3481 tggggatcgg atactccgct ataccaggat ttagtcagca gaacgcgagc cgcactggca
     3541 aaaaatccgc agaacaaatt cctcggcgta tgctggatgc aaggcgaatt tgacttaatg
     3601 accagtgact acgcgtcaca ccctcaacac tttaatcata tggttgacgc ctttcgtagg
     3661 gctctaaaac aataccattc tcagcttaat aaaattactg acgcgccgtg gttttgcggc
     3721 gataccacct ggtactggaa agaaaatttc cctcatgcgt atgaagctat ttatggcaat
     3781 tatcaaaata atgttttagc caatattatt ttcgtcgact tccagcaaca aggtgaaaga
     3841 ggactgacga acgtgcctga tgaagacccg gacgatttaa gcacgggata ttacggttca
     3901 gcgtaccggt caccggagaa ctggacgacg gcactgcgaa gcagtcattt cagcgcggca
     3961 gcccgtcgag ggattatttc taacaggttt gtagaagcaa ttttgcagtt ttggcgcgaa
     4021 aggtgagcgt tcattaattt gatttattcg catccatcag cgcttttaac tgctccggcg
     4081 taaacggtgg tttgcgtcgg tgcaacatcg catggcaatt agggcacaca ggaagtaaaa
     4141 aaacatatga gatcaaggta atatatcttc gcttgcaggc ttgaacagca gaaaatttgc
     4201 gagcatgatc gcaattattt cactgccagg gatatcaatg gcttccagta aatcgctaca
     4261 gcaggcaatt gccaacataa aaatctggca caagggtgaa cagcgcgcgc cgcataaacc
     4321 attgttattg ctatacgtat tagcgggata cctgaatgga catccacgcc ttttcgatta
     4381 tggctcggaa atctacgaac ctctgcacag tttactggaa cgttttggtc cacagcgttc
     4441 acagtaccga cctgatatgc cgttctggcg attacaaggc gacggattct ggcaattacg
     4501 caatgccgag ctttgctcaa cagcaggtag cagccgacaa ccgccagtga aggaactgac
     4561 cgaacaccgc gttgcaggcg gtttcgatga acagcactac gccctggtaa ccggcaataa
     4621 aaaactcatc aataccctgg cgcaacagat actcgaagcg cacttcaccg agagtattca
     4681 ggaagaaatt gctgacgagc tgggttttga tctccagcaa atccgtaaac agcgcgaccc
     4741 gttatttcgc aaaaatgtat tacgcgctta taaataccag tgcgcgatat gtggctttaa
     4801 tatgcgccac gacgacacca ccgtcgcgtt ggaagcggcg catataaaat ggaaacagca
     4861 cggcggtcct tgtgaaattc ctaatggcct ggcgctttgc gcaattcacc ataaagcctt
     4921 tgataaagga tcgattggcc tggacgaaaa tatgcgggta ctggtatcag atgcggtaaa
     4981 cggcggaggg atcgttgaga gattattctg ggattttgac gggaaaacta ttacgttgcc
     5041 gcaggtgcgt aaaaattacc cttttgaggg gtttgtggag tggcatcgga aagaggtttt
     5101 tagggggtga ggttgataac tatcgcagta tgttcaatat tgctgacata atcagtgcag
     5161 gatcttgcca ctagtttaca actcgccttc gatagcgacg caaaacgagt tctgttgccg
     5221 atatcctcgg ctatggatat tccaacagtt ccggcagagt tgtttaccaa gtttcaggta
     5281 agtttttact cagaaccggt tgatgcggtt tataaggcgc tggatgtgaa ttaacacatt
     5341 cctcagctta tttacaaaag gattattccc atttgatata atatcagtgc caatttactt
     5401 ttttcaatgg aatatatctc ggctaaataa caataaaact tatcgctaat ggattaaata
     5461 aacaattttc taatttattt caccaccttt gatatagaaa ttgatggtgt tgtcgctgcc
     5521 attgcttatg gtgatgataa aatccacgtt tctggatcgg cgcgtagaaa atcatcacta
     5581 ccttgatatt ttgatgcgat atgatcatac cgttccgtag taccaatttc aacttcaggc
     5641 caaaccccaa cattaa
//
LOCUS       DAZKHK010000215         5550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKHK010000215 DAZKHK010000000
VERSION     DAZKHK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.216"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..399
                     /gene="yiaB"
                     /locus_tag="ACHOS5_004846"
     CDS             58..399
                     /gene="yiaB"
                     /locus_tag="ACHOS5_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP9779531.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            445..882
                     /gene="yiaA"
                     /locus_tag="ACHOS5_004847"
     CDS             445..882
                     /gene="yiaA"
                     /locus_tag="ACHOS5_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP9779532.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(924..1919)
                     /gene="wecH"
                     /locus_tag="ACHOS5_004848"
     CDS             complement(924..1919)
                     /gene="wecH"
                     /locus_tag="ACHOS5_004848"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP9779533.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAVSLLLSMLVQRIDRNRLVS"
     gene            2094..2393
                     /gene="ysaB"
                     /locus_tag="ACHOS5_004849"
     CDS             2094..2393
                     /gene="ysaB"
                     /locus_tag="ACHOS5_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP9779534.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            2488..3399
                     /gene="glyQ"
                     /locus_tag="ACHOS5_004850"
     CDS             2488..3399
                     /gene="glyQ"
                     /locus_tag="ACHOS5_004850"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9779535.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            3409..5478
                     /gene="glyS"
                     /locus_tag="ACHOS5_004851"
     CDS             3409..5478
                     /gene="glyS"
                     /locus_tag="ACHOS5_004851"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9779536.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGNVIAD
                     YEERKAKIKADAEDAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         1399 a         1358 c         1439 g         1354 t
ORIGIN      
        1 cagttttgtc tttcgttggt tactcgtaat gtatcgctgg tagatatgga gatcgttatg
       61 aaaacctcaa agactgtggc aaaactatta tttgttgtcg gggcgctggt ttatctggtt
      121 gggctatgga tctcatgccc attgttaagt ggaaaaggtt attttcttgg cgtgttaatg
      181 acggcaactt ttggcaacta tgcatatctt cgcgcagaaa aacttgggca actggatgat
      241 ttttttatcc acgtctgtca gttggtagcg ttaatcacta tcggtctctt gtttatcggt
      301 gttttaaacg cacctatcaa tgcttatgaa atggtgatct accccatcgc cttttttgtc
      361 tgcttgtttg gtcaaatgcg tttgtttcgc tcggcatgag caacataaag ttcttacaca
      421 ttcaggaatg aaaggaatac tgtgatggac aacaaaatat caacctattc tccggcattt
      481 agtattgttt catgggtagc ccttattggc ggtattgtta cctatctgtt aggactatgg
      541 aatgcagaga tgcagttaaa tgaaaaaggg tattattttg ccgtactggt attaggtttg
      601 ttttccgcgg cgtcttatca aaagaccgtt cgggacaagt atgaaggcat acctaccacc
      661 cccatttatt atatgacttg cctgacggtc tttattatct ctgttgcgtt gttgctggta
      721 ggtctatgga atgcaacgtt attactcagc gaaaaaggct tttatgggct ggctttcttc
      781 ttaagcttgt ttggtgcagt agcggtgcag aagaatattc gtgatgccgg aataaaccca
      841 ccaaaagaaa cacaggttac ccaggaagaa tacagcgaat aattcacgta agcccggtca
      901 gtccaatgtg accgggcttc tacttaactc actaatctgt ttctgtcgat tcgttgtacc
      961 agcatagaaa gtaataaact cactgccagg gtcgcgcaaa agatccaaat aatatcgagt
     1021 attggccagt ttttaagctc aatcccccgg gtgcgcagtg catggataat caatgcatgg
     1081 aacccgtata tacctaacga atggcgggag attaagccaa gtccgcaaat agtacgcgta
     1141 tacagcgtgt ttttaaccag agtcaataac gcgattgcgc agataaaaac catcggccca
     1201 cagtaaagat accaggtatc ggcaaaattt ccgcgccact gtaattcata taatgtcccg
     1261 cgagagataa taaaaacccc cgtcgcaaac agtgcggcgc tcacccacga cagtgcttta
     1321 tgctgtgtgt ccatcatccc tatagcgcgg cccaacatgc cataaaggat gtagtaaaaa
     1381 gtatcgccat tgatatataa gttaattggc agccattcaa aaccgtcgat tttctgcggc
     1441 acggtgtttg ggttagcgat gataccaatc accaccatta gcaccagcaa catttttccg
     1501 ccgacgttct tcacctgaat cagcggtgaa accagataaa tcactgcaat cgcgaagaaa
     1561 aaccataaat ggtaaaacac tggcttttgc agcaggtttt tcagcgctaa ctccacattg
     1621 atggaggtaa acagcgcaat gtagagcagc gcgattgcgc tataaaaaaa caggcataag
     1681 ccgatacgca agaaatggcg cgactgggcg ctgcgttcgc caaaaaagag atagccggaa
     1741 atcatgaaaa atagcggcac gctgacacga gaggcagaat tcagaacatt ggcgatatcc
     1801 caggtgatgg ggctaacact atgagcattg gtcacatacc aggtagtggt gtgaatcatc
     1861 accaccatta aacacgctat ccctcgcagg ttatcaatcc agtaaatttt gggctgcatc
     1921 tgtgtctctg tatctggtta aaaaaagtct gaccgataaa tcattcagtt ggcgcactgg
     1981 aataatctga gttttatcac tacagcttat agaggcttaa ggaaattcgt aagatatcag
     2041 ccactatacc gatataaata ataagactca cctgcaaacc agacggtaat ttaatgatga
     2101 tgaacgcttt ctttccagca atggcgctta ttgtgctagt gggttgttct acaccgccac
     2161 ccgtgcagaa agcacaacgg gtaaaggttg atcctctgcg ttcgttgaat atggaagcgt
     2221 tatgcaagga tcaggcggca aaacgttata acaccggcga gcaaaaaatc gacgtcaccg
     2281 ccttcgaaca gttccaggga agctatgaaa tgcgcggtta taccttccgg aaagagcagt
     2341 ttgtctgttc ttttgacgcg gatggccatt ttttgcatct ttccatgcgt taagccctgc
     2401 tttttcccgt ttcgtactgt atatcttcca tccagcgggt atactgatcc cttcctttaa
     2461 atccacacgt atccagcacg aaataatatg caaaagtttg ataccaggac cttccagggc
     2521 ttgatcctga ccttacagga ttactgggct cgccagggct gcaccattgt tcaaccattg
     2581 gacatggaag tcggcgcggg aacctctcac ccaatgacct gtctgcgcgc gctggggcca
     2641 gaaccgatgg cggctgctta tgttcagcct tctcgtcgcc cgaccgatgg tcgctacggc
     2701 gaaaacccca accgtttaca gcactactat cagttccagg tggtcattaa gccatcgccg
     2761 gacaatattc aggagctgta cctcggttct ctgaaagagc tgggcatgga cccgaccatc
     2821 cacgacatcc gtttcgtgga agataactgg gaaaacccga cgctgggtgc ctgggggctg
     2881 ggctgggaag tgtggctgaa cggcatggaa gtgacgcagt tcacttactt ccagcaggtt
     2941 ggtggtctgg agtgtaaacc ggttaccggc gagatcacct atggtctgga acgtctggca
     3001 atgtacattc agggcgtaga cagcgtttac gacctggtct ggagcgacgg cccgctgggt
     3061 aaaaccacct acggcgacgt gttccatcag aacgaagtgg agcagtccac ttacaacttc
     3121 gaatacgcgg atgtggactt cctgttcacc tgctttgagc agtacgagaa agaagcgcag
     3181 caactgctgg cgctggaaaa tccgctgccg ctgccagcct acgagcgtat tctgaaagcc
     3241 gcccacagct tcaacctgct ggatgcgcgt aaagccatct ccgtcaccga gcgtcagcgc
     3301 tacattctgc gcattcgcac cctgaccaaa gcagtggcag aagcatacta cgcttcccgt
     3361 gaagccctcg gcttcccgat gtgcaacaaa gataagtaag aggcggctat gtctgagaaa
     3421 acttttctgg tggaaatcgg cactgaagag ctgccaccaa aagcactacg cagcctggct
     3481 gagtcctttg ctgcgaactt tactgcggag ctggataacg ctggcctcgc acacggcacc
     3541 gttcaatggt ttgctgctcc gcgtcgtctg gcgctgaaag tagctaacct ggcggaggcg
     3601 caaccggatc gtgaaataga aaaacgcggc ccggcaattg cccaggcgtt cgacgctgaa
     3661 ggcaaaccga gcaaagcggc agaaggctgg gcgcgtggct gcggtattac cgttgaccag
     3721 gccgagcgtc tgaccactga caaaggcgaa tggctgctgt atcgcgccca tgtgaagggc
     3781 gaaagcaccg aagcactgct gccgaatatg gttgcgactt ctctggcgaa actgccgatc
     3841 ccgaaactga tgcgttgggg cgcaagcgac gtgcacttcg tgcgtccggt tcacaccgtg
     3901 actctgctgc tgggcgacaa agttattccg gctaccattc tgggtattca gtccgatcgc
     3961 gtgattcgcg gccaccgctt tatgggcgag ccggaattca ccatcgataa cgccgatcag
     4021 tatccggaaa ttctgcgtga gcgcgggaac gtcatcgccg attacgaaga acgtaaggcg
     4081 aagattaaag ccgatgccga agacgcagca cgtaagattg gcggtaacgc tgacttaagc
     4141 gaaagcctgc tggaagaagt ggcttcgctg gtggagtggc cggtcgttct gaccgcgaaa
     4201 ttcgaagaga aattcctcgc ggtgccgtct gaagcactgg tttacaccat gaaaggtgac
     4261 cagaagtact tcccggtgta tgcgaacgac ggcaaactgc tgccgaactt tatcttcgtt
     4321 gccaacatcg aatcgaaaga tccgcagcag attatctccg gtaacgagaa agtcgttcgt
     4381 ccacgtctgg cggatgccga gttcttcttc aataccgacc gtaaaaaacg tcttgaagat
     4441 aacctgccgc gcctgcaaac cgtgttgttc cagcaacagt tggggacgct gcgcgacaaa
     4501 actgaccgca ttcaggcgct ggctggctgg attgctgagc agattggcgc tgacgttaac
     4561 cacgctaccc gtgcgggcct gctgtctaag tgtgacctga tgaccaacat ggtcttcgag
     4621 ttcaccgaca cccagggcgt tatggggatg cactacgcgc gtcacgatgg cgaagcggaa
     4681 gatgtcgcgg tggcgctgaa tgagcagtat cagccgcgct ttgccggtga tgacctgccg
     4741 tccaacccgg tagcctgtgc gctggcgatt gctgacaaga tggataccct ggcgggtatc
     4801 ttcggtatcg gtcagcatcc gaaaggcgac aaagacccgt ttgcgttgcg tcgtgccgca
     4861 cttggcgtgc tgcgtattat cgttgagaag aacctcaacc ttgatctgca aacgctgacc
     4921 gaagaagcgg tgcgtctgta tggcgataag ctgactaatg ccaacgtggt tgatgatgtt
     4981 atcgacttta tgctcggtcg cttccgcgcc tggtatcagg acgaaggtta caccgttgat
     5041 accatccagg cggtactggc gcgtcgtccg acgcgtccgg cagatttcga tgcccgtatg
     5101 aaggcggtat cgcatttccg taccctggat gcagctgctg cactggcggc ggcgaacaaa
     5161 cgtgtatcca acattctggc gaaatctgac gaagtgctga gcgaccgcgt gaatgcttct
     5221 accctgaaag agccggaaga aattaaactg gcgatgcagg ttgtggtgct acgtgacaag
     5281 ctggagccgt actttgcgga aggtcgttac caggatgcgc tggtcgaact ggcagagctg
     5341 cgtgagccag ttgatgcttt cttcgataaa gtgatggtca tggttgatga caaagaattg
     5401 cgtctcaacc gtctgaccat gctggagaaa ctgcgcgaac tgttcctgcg cgttgcggat
     5461 atttcgctgt tgcagtaata acgccgttat taaatagcct gccatctggc aggctttttt
     5521 tatcgctaaa taatacagca acctttaata
//
LOCUS       DAZKHK010000216         5483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKHK010000216 DAZKHK010000000
VERSION     DAZKHK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.217"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            207..1421
                     /gene="manD"
                     /locus_tag="ACHOS5_004852"
     CDS             207..1421
                     /gene="manD"
                     /locus_tag="ACHOS5_004852"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFP9779537.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1488..2771
                     /locus_tag="ACHOS5_004853"
     CDS             1488..2771
                     /locus_tag="ACHOS5_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9779538.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3023..3940
                     /gene="oxyR"
                     /locus_tag="ACHOS5_004854"
     CDS             3023..3940
                     /gene="oxyR"
                     /locus_tag="ACHOS5_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP9779539.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3923..5323)
                     /gene="sthA"
                     /locus_tag="ACHOS5_004855"
     CDS             complement(3923..5323)
                     /gene="sthA"
                     /locus_tag="ACHOS5_004855"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP9779540.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1235 a         1260 c         1494 g         1494 t
ORIGIN      
        1 atggagtttg ttatgtgatt atttaacgtg ttgttttaac aatggtggct aattgttcag
       61 gtggtttttg agggtgtttt ttctcctcgg tagaaatttt tatttttaat gtgatcacgg
      121 ttgaataatc actttttctc tcttgtatgg tagtaggcgt aaaattaagt ttgaagtgtc
      181 tatttacaat acaggagatt gcaataatga aaattattgc tgtggatgtt tttgttactt
      241 gtcccgggcg taatttcgtt acgcttaaaa taaccactga aagcggactt tgtggattag
      301 gagatgccac gttaaatggt cgcgaattgt cggtcgcttc ttatctgaaa gatcatcttt
      361 gtcctcaatt aattggccgc gatgccagcc gtatcgaaga tatctggcaa ttcttttata
      421 aaggagctta ctggcgacgt ggtcccgtca cgatgtcggc catttctgcc attgatatgg
      481 cgttgtggga tatcaaagct aaagcggcaa atatgccatt gtatcaactg cttggtggcg
      541 cttctcgtga aggcgtgatg gtttattgcc acaccaccgg gcggacgatt gatgaagtcc
      601 ttgaagacta tgcaaagcac cagcagatgg gctttaaggc gattcgtgtg cagtgtggtg
      661 taccgggtat gcaaaccacc tatggtctgg cgaagggcaa aggactggcg tatgaaccag
      721 caaccaaagg cctgtggcca gaagagcagt tgtggtcttc agaaaagtat ctcgatttta
      781 cgccgaagtt atttgaagcg gtgcgtaata agttcggttt taacgaacat ctgttgcatg
      841 acatgcacca tcgcctgacc cccatcgaag cggcgcgctt tgggaggagc attgaggatt
      901 accgcctgtt ctggatggaa gatccgacgc ctgccgagaa ccaggaatgt ttccgtctga
      961 ttcgccagca taccgtgacg ccgattgcgg tcggtgaagt gtttaacagt atctgggatt
     1021 gcaagcagtt aatcgaagag caactgattg actatattcg cacgacgatt acccatgcag
     1081 gcggcattac cgggatgcga cggattgccg attttgcatc gctttatcag gtacgtaccg
     1141 gttcgcatgg tccttcagat ttatcgcctg tttgtcatgc ggcggcgctg cattttgacc
     1201 tctgggtgcc aaactttggc gttcaggagt acatgggcta ttccgaacaa atgcttgaag
     1261 tcttcccgca tagctggcgc ttcgatgaag gttatatgca cccaggcgat gaaccggggc
     1321 tgggtatttc tttcgatgag aaactggcgg cgaaatatcc gtatgacccg gcgtatctcc
     1381 ccgtcgcacg actggaagat ggcacattgt ggaactggta ataacagttc gcttaattat
     1441 tttctgaata gtcatcaaat cgattaattc cgctcaagga gctaaaaatg gacgttgatg
     1501 tgtcaaccag tgttgcgggt aataaaccgc aacggattcg tcgtattcag acagtaacgc
     1561 tggttttatt atttatggcg gggatcgtta atttcctcga ccgttcgtca ttgagcgtgg
     1621 caggcgaagc gattcgtggc gagttaggat tatcggcgac agaatttggc gttttgcttt
     1681 ctgcattttc tctgtcttat ggtttttcac aactcccttc cggtattttg ctagaccgct
     1741 ttggcccacg aatagtttta ggcgcaggct taatattctg gtcattaatg caggcattaa
     1801 caggaatggt taattctttt agccacttta ttttaatgcg tatcggtctg ggtattggcg
     1861 aagcgccatt tatgcctgcg ggagtaaagt cgatcaccga ctggtatgcg caaaaagaac
     1921 gcggcacggc gctggggatt tttaactcat ctaccgttat cggtcaggct attgcgcctc
     1981 ctgctcttgt attgatgcaa ctggcatggg gctggcggac gatgtttgtc atcatcggcg
     2041 tagcagggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg gcgcagtttg
     2101 tcctgaccga ggaagaacgg acatatctct ccgcgccggt taaaccgcgt ccacagttac
     2161 aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg attttaggtt
     2221 tctctggcgt caattatacc ggttggctct acatcgcgtg gttacccggt tatttgcagg
     2281 ccgaacaagg tttcagtctg gcgaaaaccg gttgggtggc ggcaattcct ttcctcgcgg
     2341 cggcggtcgg gatgtgggtt aacggtattg ttgtcgatcg gctggcgaaa aaaggctatg
     2401 acctggcgaa gacgcgtaaa acggcgattg tctgcggttt gatgatgtcg gcattaggca
     2461 cgttgctggt cgtgcaatct tcctcgccag cccaggcggt agcgtttatc tcaatggcgc
     2521 tgttctgcgt gcatttcgcg ggaacatctg catgggggct ggtgcaggtg atggtgtcag
     2581 aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt tttgcttcct
     2641 ttgctccgat cgtaaccggt tgggtcgtgg ataccacaca ctcgtttaat ctggcgctgg
     2701 ttatcgcggc ctgtgtgacg ttcaccgggg cgctgtgtta cttctttatt gtcaaagatc
     2761 ggattgagta accgttacta tcaggctctc ttgctgtggg cctgtagaat aaaaaaaagc
     2821 ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg agaaactctc
     2881 gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt gagcaattat
     2941 cagtcagaat gcttgatagg gataatcgtt cgttgctatg ctacctatcg ccgcgaacta
     3001 tcgtggcaat ggaggatgga taatgaatat tcgtgatctt gagtacctgg tggcattggc
     3061 cgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc cgacgcttag
     3121 cgggcaaatt cgtaagctgg aagatgagtt gggcgtgatg ttgctggagc ggaccagccg
     3181 taaagtgttg ttcacccagg cgggaatgct gctggtggat caggcgcgta ccgtactgcg
     3241 tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt ccggaccgct
     3301 gcatattggt ctgattccca cggttggacc gtacctgcta ccgcatatta tcccgatgct
     3361 gcaccagacc tttccaaagc tggaaatgta tctgcatgaa gcacagaccc accagttact
     3421 ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga aagagagcga
     3481 agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct atgaagacca
     3541 cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa aactgctgat
     3601 gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgttttg aagccggggc
     3661 ggatgaagat acacacttcc gagcgaccag cctggaaact ctgcgcaaca tggtggcggc
     3721 aggtagcggg atcactttac tgcctgcgct ggctgtgccg ccggaacgca aacgcgatgg
     3781 ggttgtttat ctgccgtgca ttaagccgga accacgccgc actattggcc tggtttatcg
     3841 tcctggctca ccgctgcgca gccgctatga gcaactggca gaggccatcc gcgcaagaat
     3901 ggatggccat ttcgataaag tgttaaaaca ggcggtttaa gccatttagc gcagctaccc
     3961 gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt
     4021 tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg gcagcgcgct
     4081 cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa attttcagcg
     4141 tgcccacgtt catgccgacg atttgtgcac gtgccagatg tttaaactgg gcgcggccca
     4201 cttcatatgg cactttcatc gcggtcagct gctgttcggt tttgcctaca gagctgattt
     4261 ccgggatggt gtaaatgccg gtagggatat cttcaatcag atgtgcggtg gcttcacctt
     4321 tcaccagcgc ctgcgcggca atgcgcccct ggtcataggc tgccgacgcc aggctcggat
     4381 aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga
     4441 ccttcagctg tccgcgactg tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg
     4501 tattaccggt gcgaccgttg gcatagagca gacagtcagc tttcagtttt ttacccgact
     4561 tcagatgcat gatcacaccg tcgtcacagc cttcgatctt ctcgtactct tcgttgtgac
     4621 gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc tcttgatcga
     4681 gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc ataccgcgga
     4741 agatcgacgc atattcacag ccgatcactc cagcaccgta aataagtaca tggcgcggtt
     4801 cgtggtgcat gctaagaatt gagtcgctgt cataaatgcg tggatgggtg aaatcaacat
     4861 ctgttggatg atatggacga gaaccgcagg caataacaaa tttttcagca gttagtgttt
     4921 cgacgctgcc gtccgggcaa tccagcgcca acgtatgctc atcaacaaag cgagcgttcc
     4981 cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat
     5041 taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc agtcgggaat
     5101 ggtcgctgta tagtgggttt tgattgaatt ctataatgcg actgacggcg tgacggagag
     5161 ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga taacgctcga
     5221 taactgcgac gcgcgcacct tgcttaacca ggcccatcgc agcgccttcg ccgccggggc
     5281 cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg cttacctatt
     5341 cttatacata aaagcaacag aatggtaaca ttttatcgcg ggtaagccaa ttgatccccg
     5401 tcatttatct ggctatatcg tgagcggcct ttgctttgtc ttcctccttc ttattttatt
     5461 tattcactat taatcgcttt ttc
//
LOCUS       DAZKHK010000217         5443 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKHK010000217 DAZKHK010000000
VERSION     DAZKHK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.218"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..82
                     /locus_tag="ACHOS5_004856"
     CDS             <1..82
                     /locus_tag="ACHOS5_004856"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9779541.1"
                     /translation="NVINEETLLQQPEERFAWLRSKKDRF"
     gene            154..1512
                     /gene="mltD"
                     /locus_tag="ACHOS5_004857"
     CDS             154..1512
                     /gene="mltD"
                     /locus_tag="ACHOS5_004857"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP9779542.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1561..2331)
                     /locus_tag="ACHOS5_004858"
                     /pseudo
     CDS             complement(1561..2331)
                     /locus_tag="ACHOS5_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(2409..3209)
                     /gene="yafD"
                     /locus_tag="ACHOS5_004859"
     CDS             complement(2409..3209)
                     /gene="yafD"
                     /locus_tag="ACHOS5_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9779543.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3450..4364
                     /gene="yafC"
                     /locus_tag="ACHOS5_004860"
     CDS             3450..4364
                     /gene="yafC"
                     /locus_tag="ACHOS5_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP9779544.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4361..5164)
                     /gene="dkgB"
                     /locus_tag="ACHOS5_004861"
     CDS             complement(4361..5164)
                     /gene="dkgB"
                     /locus_tag="ACHOS5_004861"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP9779545.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5327..5403)
                     /locus_tag="ACHOS5_004862"
     tRNA            complement(5327..5403)
                     /locus_tag="ACHOS5_004862"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5367..5369),aa:Asp,seq:gtc)
BASE COUNT         1434 a         1313 c         1348 g         1348 t
ORIGIN      
        1 taatgtaatt aatgaagaaa cattattgca acaacctgaa gaacgttttg catggttaag
       61 gtcaaagaaa gataggttct gataaaactt tcttgtcatc ggctccgttc gccgttatga
      121 tcggtcgtct tttaagcaac tattgacaca cacatgaagg caaaagcgat attactcgcc
      181 tctgtcctgc tcgtgggttg ccagagcacc ggcaacgttc aacagcacgc acagagcctt
      241 tctgcagctg gtcaagggga agcagcaaag tttacaagtc aggcacgatg gatggacgat
      301 gggacgtcta tcgcgccaga tggtgacttg tgggctttca ttggcgacga gctaaagatg
      361 ggaattccgg aaaacgaccg gattcgcgaa cagaaacaga aatatttacg caataagagc
      421 tatctccacg atgtaacttt acgggcagag ccgtatatgt actggatagc cgggcaagtt
      481 aaaaaacgta acatgcctat ggaactggta ctactaccca tagtggagag cgcttttgat
      541 cctcacgcaa cgtctggcgc caatgccgcg ggcatctggc agatcattcc gagcacgggg
      601 cgcaattatg gtttgaaaca gacccgcaat tatgacgcgc gtcgcgatgt tgttgcttca
      661 acaactgccg cgctgaacat gatgcagcgt ctgaacaaga tgtttgacgg cgactggctt
      721 ctgaccgtag cggcttataa cagcggcgaa ggtcgggtca tgaaggcaat taaaacgaac
      781 aaagcgcgtg ggaaatccac ggacttctgg tcgttaccgt tgccgcagga aacgaagcag
      841 tacgtgccta aaatgctggc attgagtgat attctcaaaa acagcaagcg ttatggcgta
      901 cgtctgccaa cgaccgatga aagccgtgct ctggcacgtg tgcacctgag cagcccggtt
      961 gaaatggcga aggttgcaga tatggcgggg atttccgtca gcaagctgaa gacattcaac
     1021 gcaggcgtga aaggctccac gctgggcgca agtggtccgc agtacgtgat ggtgccaaag
     1081 aagcatgcag atcaactgcg tgaatctctg gcttcaggcg aaattgctgc tgtacagtcg
     1141 acgctggttg ccgacaatac gccgcttaac agccgtgttt acaccgtacg ctctggcgac
     1201 acgctttcaa gtatcgcttc acgtctcggc gtaagcacca aagatttgca gcagtggaac
     1261 aaactgcgcg gatctaagct gaagccaggc caaagtttga cgattggtgc aggcagtagc
     1321 gcacagcgac tggcaaacaa cagcgatagc attacgtatc gtgtgcgcaa aggcgattcg
     1381 ctttcaagca ttgctaaacg ccacggcgtg aacatcaaag atgtaatgcg ctggaacagc
     1441 gacactgcga atctgcaacc aggcgataag ctgacgttgt ttgtgaaaaa caacaacatg
     1501 ccagattcct gacaaaccag ataataaaaa aggcaccgat tcccccggtg ccttttttat
     1561 ttatgccgct ttatgtgctt ctagcatgat gatatcactg gtgaaagagc catcattctg
     1621 caaggcaaaa tacgttttca cctctgtcga tgcgctctgt tggtaaatgc gaatagcgtc
     1681 tactaacgct tctggcgtac gcattctcgc tacccatgaa gaaaattcca gcggtaactt
     1741 atctgtaatt aaattatcaa ctatcagatt ggcttcactg attaacgtca accactcacc
     1801 gctggcgtag tttcgtacgt gagaggtatc gcgtaatgct tctaccgtct gtaaccagat
     1861 gtcgcgcact ggatgacccg gagacattac gtccatcaca atcagcctac cgccaggttt
     1921 caatatccta ttcacttctc gcagtgctgc accaacatca tgccagtgat gggcagaata
     1981 acggctgata acaatatcaa atgcgttatc ggcaaatggc aggctttcga cataacccta
     2041 gcgggtggtg atatttttca gttgccgggc ttcggcagct tgtgccacaa catccagcat
     2101 atgggcagat aagtcatacg ccaccaccgt gctcacgttt tgcgcagcga caaagctggc
     2161 atgccctgct ccgcagccca tatcaagtac acttgcatca ggataatcag ccagacgcac
     2221 cgccagacgc tgtaaatctc gtccggatgc atgtacggta ctggtcagat attcgctggc
     2281 ttgagagcta aactgctttt ctacgtggtc atggtgggat tgtgttgtca ttgcgctgtc
     2341 cttttgttat ttgagtataa aagggcagcg ccaaacgttg gtctgccctg tggcagacct
     2401 gacatacctt atttatcagg cttgccggga ctgaattcaa cgagtagcgg attgtgatcg
     2461 gacgcgcgcg taaccagtac agaagcttcg ctgacgttca gaccacggta gaaaacaaaa
     2521 tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac ctggcgcagc
     2581 gacatttccc gtgcaaagcg atataacgcg ttcatccttc tacggctcca ggcattgaaa
     2641 tctcccgcca taatgaccgg gccactgtgg tgagctatct gatcgccaat aggaagtaac
     2701 tgcttactat agacatccac gcccagactg aagttgacgg cgtgtatatt aaccaccatc
     2761 aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg ccgacttcgc cagacgcaaa
     2821 atgggttctc gttcacgtaa cgggcagcaa tacactggat gtgccgccga aagggtcatt
     2881 acgccagaag gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc aagatagtta
     2941 gcagtcgcaa actgtactaa ctctggcgtt gtctgcgctt cctgcaataa caccagatgt
     3001 gcatctttgc cgtagttctt taataccgac aaccattcag cgcgttgctg tttgtatatg
     3061 ttccacacca ggatccgaat acgctcttcg gtacttaacg gttctccagg tggtaatgcc
     3121 tggccgatgc tcgcaaaaga ccccggcggt aagatccttt ccgcaggttg tccggcaaca
     3181 tagcgcatgg cataggtgtt ttttcgcact tttgactttg aaacctctat cacgtcaacc
     3241 cttccgcact cgggagggtt ctactgttat agggatttta gctcactctt tcaacgagca
     3301 attgtatatt gttatgtaag caagtgctta caccggtcag gattttgctc attattgaga
     3361 acattaaatc ggatggcata tctcgcatcc tgatggttta atgacaaata aattcaaaat
     3421 atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa ctcgccattt
     3481 ttgtttcagt cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta gggcaagcaa
     3541 actcagcggt aagccgggcg gtgaaaaagc tggagatgaa acttggcgtt agcctgctta
     3601 atcggaccac gcggcaactt agcctgacgg aagaaggcga gcgttatttt cgtcgcgtac
     3661 agtcaatttt gcaggagatg gcagcggcag aatcagaaat tatggagacg cgtaatacac
     3721 cgcgtggact gttacggatc gatgccgcaa ctccagtggt gctgcacttt ctgatgccgt
     3781 tgattaagcc ttttcgtgaa cgctatccgg aagtcacttt atcgctagtc tcctccgaaa
     3841 cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat acgcgccggt acgttaacgg
     3901 attccagctt acgtgccagg ccgttattta acagttatcg aaaaattatc gcctcccccg
     3961 attatatttc ccgctacggg aagccagaaa cgatcgacga tttaaagcaa catatttgcc
     4021 tgggattcac tgaacccgct tccctcaata cctggccgat agcctgtagc gatggacaat
     4081 tacatgaggt gaagtacggt ttgtcctcca atagtgggga aacactgaaa cagctttgcc
     4141 tgagtggcaa cgggattgcg tgtttgtccg actacatgat cgacaaagaa atcgctcgcg
     4201 gagaattggt ggagttaatg gcagataaag tgttgccagt ggaaatgcca ttcagtgcag
     4261 tctattacag cgaccgtgcg gtaagtacgc gcatccgggc ttttatcgat ttccttagcg
     4321 agcatgtaaa aacagctccc ggaggagctg tcagagaggc ttaatcccat tcaggagcca
     4381 gaccttccgg gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct tttttatctt
     4441 cggcatcaag ctgtaaattt tgtgccttaa gattactctc caggttttca cgtttagtag
     4501 aagaaggaat tactgagtaa ccttccccca tagcccacgc cagaatcact tgagccggag
     4561 tcgcattgtg tttagctgca atacgagcaa taaccttatc tttcagtgca ttgccatacg
     4621 ccagagtcat ataggaagta atatggatac cgtgctgttt agcccaagcg accactttac
     4681 ggttttgcag ataaggagag agttcaatct ggttagtagc gatgttttca gcgccaacag
     4741 cagcaatcgc tttttccatc aatgggatcg tgaagttgga aataccgatc tcacgcgtca
     4801 gcccttgttt tttggcttcc agcagcgcct gcataaactc ttcagcagag acttcatcgc
     4861 ttggtgacgg ccagtggatt agcgtcagat caacataatc ggtacgcaat ttttgcaggc
     4921 tctctttcag actcgggatc aatttgtctt tgctgagatt ttcaatccag attttagtgg
     4981 tgatgtagag ttcatgacgt ggcacgccac tttctgcaat cgcctgacct actgcggctt
     5041 cgttatcata gatttgtgcg gtatcaattg cgcgataatc aagttcaagc gccgttttca
     5101 cagatgaaat aacaacgtcg tctttcagac ggaaagtgcc taaaccaaat gcagggatag
     5161 ccataatatg cctcttttaa tgctattttc gttaactgag gtgattatgg gcttcagact
     5221 gttgtggaaa aatggcgtaa aacgcagagg atttttgcga ttttggcaat aatagaaata
     5281 caaacgaaaa aaaacctcga gcgttaactc gaggcttctt aataagtggc ggaacggacg
     5341 ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc
     5401 tccaccgaat tcttttacaa ccaccggttt ataaccggct tac
//
LOCUS       DAZKHK010000218         5409 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKHK010000218 DAZKHK010000000
VERSION     DAZKHK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.219"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            145..1941
                     /locus_tag="ACHOS5_004863"
     CDS             145..1941
                     /locus_tag="ACHOS5_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFP9779546.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAIIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVDISYKVGNESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKAMKDDYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSNKPNQTHYRALLVRPQQMFK"
     gene            1959..2627
                     /locus_tag="ACHOS5_004864"
     CDS             1959..2627
                     /locus_tag="ACHOS5_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFP9779547.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2642..3313
                     /gene="dsbI"
                     /locus_tag="ACHOS5_004865"
     CDS             2642..3313
                     /gene="dsbI"
                     /locus_tag="ACHOS5_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HFP9779548.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(3359..4147)
                     /gene="ygiD"
                     /locus_tag="ACHOS5_004866"
     CDS             complement(3359..4147)
                     /gene="ygiD"
                     /locus_tag="ACHOS5_004866"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP9779549.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTYYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4290..5063
                     /gene="zupT"
                     /locus_tag="ACHOS5_004867"
     CDS             4290..5063
                     /gene="zupT"
                     /locus_tag="ACHOS5_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP9779550.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTVGIG"
     gene            complement(5125..5295)
                     /locus_tag="ACHOS5_004868"
     CDS             complement(5125..5295)
                     /locus_tag="ACHOS5_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779551.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1425 a         1313 c         1294 g         1377 t
ORIGIN      
        1 aacaacatat ttaaaagaaa cacaaaaaca ttctcactct ttattgaatt aatatttata
       61 ttcacgtaac gaatttatta gcaccctctc aacagtcgat attcatcatt attatatatt
      121 tgcgttatat aaggagatat atccatgttt gataaatata gaaaaacact cgtagccgga
      181 actgtggcga taaccctggg tttgtcagca tcgggggtga tggctgcggg ttttaaacca
      241 gcgccgcctg ccgggcagct tggggcgatt atcgtcgatc cctacggtaa tgcgccctta
      301 accgcgctgg ttgatttaga cagccatgtt atttctgacg tcaaagttac cgttcacggg
      361 aagggcgaaa aaggcgttga catcagctat aaagtgggca atgagtcact gaaaacttac
      421 gatggtgtac cgatttttgg tctttaccag aaatttgcga acaaagtgac cgttgagtgg
      481 aaagaaaacg gcaaggccat gaaagatgat tacgtggtgc agacttccgc catcgtcaac
      541 aattacatgg ataaccgctc catctccgac ctacagcaaa ccaaagtcgt taaagtcgcg
      601 ccaggttttg aagatcgcct ctatctggtt aatacccaca cctttaccgc ccaaggttcc
      661 gatctccact ggcatggtga gaaagataaa aatgccggta tccttgatgc gggtccggca
      721 actggcgcac tcccttttga tatcgcgcca ttcaccttta tcgtcgatac cgaaggtgaa
      781 taccgctggt ggctggatca agacactttc tacgatggtc gtgaccgcga tatcaacaag
      841 cgtggttatc tgatgggtat ccgcgaaacg ccacgcggca cctttaccgc tgtacaaggt
      901 cagcactggt acgagttcga catgatggga caggtgctcg aagatcacaa actaccgcgc
      961 ggatttgctg acgccactca tgagtccatt gagacgccaa atggcacggt actgttgcgc
     1021 gtaggtaaga gtaactatcg tcgcgatgac ggcgtacacg tcaccaccat tcgtgaccat
     1081 atcctcgaag tcgataaatc tggtcgcgtt gtcgatgtat gggatctgac caagatcctc
     1141 gatccgaaac gcgatgcact gctcggcgcg ctggatgcag gtgcagtttg cgttaacgtt
     1201 gaccttgccc atgcaggaca acaggcaaaa ctggaaccag atacaccgtt cggcgacgcg
     1261 ctgggtgtag ggccaggccg caactgggcg cacgtgaact ctatcgctta tgacgcaaaa
     1321 gatgactcta tcatcctctc ctctcgccac cagggtgttg tgaagattgg tcgtgataag
     1381 caagtgaaat ggatccttgc gccctctaaa ggttgggaaa aaccgctggc cagcaagctg
     1441 ctgaaaccgg tggacgctaa cggtaaacct attacctgta acgaaaacgg tctgtgtgaa
     1501 aactcggact tcgattttac ctacacccag cataccgcct ggatttccag caaaggaacg
     1561 ctcaccattt ttgataatgg cgatggtcgt catctggaac aacctgcctt accaaccatg
     1621 aaatattccc gctttgtgga atataagatt gatgagaaga aaggcaccgt tcagcaagtg
     1681 tgggaatacg gtaaagaacg tggctacgat ttctatagcc cgattacctc catcattgaa
     1741 tatcaagccg accgtaacac catgtttggc ttcggtggtt ctattcattt gttcgatgtc
     1801 gggcagccaa ccgtcggtaa gttgaacgaa atcgattaca aaaccaaaga agtgaaagtg
     1861 gaaatcgacg tgctgtcaaa taaacccaat cagactcact accgtgcgct gttagtccgt
     1921 ccacaacaga tgttcaaata attaagaagg gaataaaaat gtcgaaatta gggatctcat
     1981 cactatttaa aaccatactg cttaccgcag ctttggctgt ttcatttacc gcttctgcat
     2041 ttaccgaagg cacggattac atggttctgg aaaaacctat tccgaatgcc gacaaaacgc
     2101 tgattaaggt atttagctac gcctgcccgt tctgttacaa gtacgacaaa gcggttaccg
     2161 gtccggtatc ggaaaaagtg aaagatatcg ttgctttcac gccgttccat ctggaaacaa
     2221 aaggtgaata cggcaaacaa gcaagcgaag tctttgcggt cttgatcaat aaagataaag
     2281 cagcaggcat ttccttattt gatgctaact ctcagtttaa gaaagccaag tttgcctact
     2341 acgcggctta tcacgacaaa aaagaacgct ggtctgacgg taaagatccg gctgctttta
     2401 tcaaaaccgg tctggatgcc gcaggtatga gtcaggcgga ttttgaagcg gcactgaaag
     2461 aaccagcggt tcaagaaaca ctggaaaaat ggaaagcctc ttatgatgtc gccaaaatcc
     2521 agggggttcc agcctatgtc gttaatggta agtacttgat ctacaccaag agcatcaaat
     2581 ccatcgacgc gatggcagac cttatccgtg agttagcaag caaataacag ggaaacaggc
     2641 tatgggtatt aagggaatgt ggaaagatct tcgcacatca ccagttgaca cactggtcag
     2701 atggcaggag caacgcttgt tgtggttgtt aatggccgtt gcgatgggcg cgttaattat
     2761 cctggcgcac tctttcttcc agatctatct ctacatggca ccctgtgagc aatgtgtata
     2821 cattcgctac gccatgtttg tgatggttat tggaggattg gttgcagcga tcaacccaaa
     2881 gaacatcatc ctaaagctaa ttggctgcgt gatggcattt tacggcagca ttttgggact
     2941 gaagttttcg ctcaaactga acgatatcca ccatgcagta cataatccgg atcccgattc
     3001 actgtttggc gtacagggtt gctctaccga tccaactttt ccctttaacc tgccgctggc
     3061 acagtgggca ccaaattggt tcaagcctac cggggattgt ggctatgacg caccaatcgt
     3121 tcctgatggc gtcacactga gcagcacgca acagtggttt gtggaaatgt atcagcaatc
     3181 tgaaggctgg tatctgctac caccatggca ttttatgaat atggctcagg cttgtatgct
     3241 ggcttttggt atgtgtctgg tgctgctggt cattatgagt ggcgcatggg cgcttaaaat
     3301 catcagaggc taactcagca acacccgaag ccagtcattc tggcttcggg ttcttttatt
     3361 aacctatctg caccgacagc atacttaaac tgcccatttc tataccatcg accggaatgg
     3421 taattggctc ctgcccatcc cacgccccta agacatacaa caacggcaga tagtgctctg
     3481 gcgttgggtt cgataacgcg ccaccttcat ggtcgagata attcaccaga ggatgttgtt
     3541 ccaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac gtcgcccacg
     3601 gatacggtga actatcaccg tgccacttca ctgtacgcag gttatgtacc acgttgccac
     3661 tggcgaccaa cattatccct tcatcacgca gcgccgcgag tttgcgcccc atttcgaaat
     3721 gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catcgggata tcggcgtcag
     3781 ggtacatctt aatcagtacg ccccacgagc cgtggtcgaa gccccaggct tctttatcca
     3841 gcgtcacagg gaccggcgct aacagctcaa ccagatgctg tgccagcgca ggtgaacccg
     3901 gagcaggata atacgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg
     3961 gcggcatctc catcgcggtc actcctgttc cacgggtaaa ccaatgagcc gaaaccacca
     4021 ctattgcttg cgggcgtggc aatgtcatcc ccaacgtctg ccagctgcgg gtatacaaat
     4081 tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg
     4141 aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa tacgcttgtt
     4201 cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc
     4261 tgaaggtaaa gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg
     4321 cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cgggcaaaaa ccctcgaacc
     4381 gcttactggc gttttcgcta ggatttgcgg cggggatcat gttgctcatc tcattaatgg
     4441 aaatgcttcc tgccgcatta gccgccgaag gaatgtcgcc tgtgttgggt tatggaatgt
     4501 ttatcttcgg tctgcttggc tattttggcc tggaccgcat gttgccacat gcgcatccgc
     4561 aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc
     4621 tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg
     4681 cgagcagcaa cctggagctg ggatttggca tcgccctggc cgtagcgttg cacaatattc
     4741 ctgaaggtct ggcggttgca ggcccggttt acgctgcaac aggatcgaaa cgtaccgcga
     4801 ttctgtgggc gggcatttcc gggctggcag aaattcttgg tggtgtgctg gcatggttaa
     4861 tcctcggtag catgatttct ccggtggtca tggcggcaat catggcggcg gttgcaggaa
     4921 ttatggtggc gctctccgtt gacgaattaa tgccgctcgc caaagagatt gaccccaata
     4981 ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttagtgctgc
     5041 tacaaacggt gggaattggt taaagacgat aatgacagat gttgcccatt gctaaaggca
     5101 acatctgtat cgttattaaa ggtattattg atgcttttca aacacttctt tatcggagcg
     5161 tttatctaat ttacttcggg cagctaacag cgcatctctg tcatggcgaa aagctttaca
     5221 gtaacgttta agatgaagaa aatagccacc agccagtaga acaattaata caatccagta
     5281 ccaggcaata aacatccttt tcaccttcaa aatttaacat tactaaacat ttagcgttaa
     5341 aatttcacat atccatcgtt gaatacattc atatactcgc ataattctgg atcaagtata
     5401 tgatttcga
//
LOCUS       DAZKHK010000219         5399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKHK010000219 DAZKHK010000000
VERSION     DAZKHK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.220"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..666
                     /locus_tag="ACHOS5_004869"
     CDS             <1..666
                     /locus_tag="ACHOS5_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9779552.1"
                     /translation="HAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDL
                     GFDDSHVVTMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPAT
                     SIIQDKAKKVLVLKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHH
                     LIGHGQGGMGTKAHDLFVLPLCRKHHDELHADTVTFEEKYGSQLELIFRFIDRALAIG
                     VLA"
     gene            680..1432
                     /gene="ydfT"
                     /locus_tag="ACHOS5_004870"
     CDS             680..1432
                     /gene="ydfT"
                     /locus_tag="ACHOS5_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9779553.1"
                     /translation="MNLEALPKYYSPKSPKLSDAAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKAGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEPCAGTGFHNVLREVVKYSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(1704..1793)
                     /locus_tag="ACHOS5_004871"
     CDS             complement(1704..1793)
                     /locus_tag="ACHOS5_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779554.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLTVIALLIM"
     gene            complement(1848..2060)
                     /gene="cspF"
                     /locus_tag="ACHOS5_004872"
     CDS             complement(1848..2060)
                     /gene="cspF"
                     /locus_tag="ACHOS5_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFP9779555.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            2361..2576
                     /gene="cspB"
                     /locus_tag="ACHOS5_004873"
     CDS             2361..2576
                     /gene="cspB"
                     /locus_tag="ACHOS5_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFP9779556.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNFRT
                     LFEGQKVTFSVESGAKGPAAANVIITD"
     gene            complement(2940..3110)
                     /gene="ynfU"
                     /locus_tag="ACHOS5_004874"
     CDS             complement(2940..3110)
                     /gene="ynfU"
                     /locus_tag="ACHOS5_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFP9779557.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
     gene            3329..3544
                     /gene="essQ"
                     /locus_tag="ACHOS5_004875"
     CDS             3329..3544
                     /gene="essQ"
                     /locus_tag="ACHOS5_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9779558.1"
                     /translation="MKSMDKLTTGIAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKVARRE"
     gene            3549..3884
                     /locus_tag="ACHOS5_004876"
                     /pseudo
     CDS             3549..3884
                     /locus_tag="ACHOS5_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(4048..4308)
                     /locus_tag="ACHOS5_004877"
     CDS             complement(4048..4308)
                     /locus_tag="ACHOS5_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779559.1"
                     /translation="MDYTNLPKQTFADLIALRQAVVALINLLPEKEKELVKALLNRTA
                     ADFSSYPLTDDLADLPELIAASAIKLTEEIYPPQKSSQNSCE"
     gene            4422..4955
                     /gene="rrrQ"
                     /locus_tag="ACHOS5_004878"
     CDS             4422..4955
                     /gene="rrrQ"
                     /locus_tag="ACHOS5_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9779560.1"
                     /translation="MNVKIRYSLSAAVLALIAASAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEH"
     gene            4952..>5399
                     /locus_tag="ACHOS5_004879"
     CDS             4952..>5399
                     /locus_tag="ACHOS5_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFP9779561.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGV"
BASE COUNT         1506 a         1049 c         1371 g         1473 t
ORIGIN      
        1 cacgctccgg gagcaattcg tctgtgctgg cactgcgata accaactgcg tgaccagttc
       61 acggaacggc tggaatcaat ggcaacggat aactgtgccc gctgggtgtt gtctgtcgtg
      121 cgtcgggatc tcggttttga tgacagtcac gttgtgacaa tgccggaact gtgctggtgg
      181 ctggttcgta atgatttggc ggatgcctta ccggaaagtg cagcccgtaa ggcactgaga
      241 ttaccgaagc ctgttgtgcc atctgtcacc cgggaaagtg accttgtgcc ttcggttcct
      301 gccaccagca tcatccagga taaagcgaaa aaggtgctgg tgctgaaagt ggatccggag
      361 tcgccggagt cttttatgtt acgccccaaa cgtcgccgct gggttaatga aaagtacacg
      421 cgctgggtta agacgcagcc gtgcgcatgt tgtggaaagc cagctgatga tccccaccac
      481 ctgataggcc acggtcaggg tggaatgggt acaaaagcgc atgatctctt tgtgttgcct
      541 ttgtgcagaa agcatcacga cgagctgcat gcggataccg tgacatttga agagaagtat
      601 ggctcccaac tggagctgat atttcgtttt atcgatcgtg cgctggcaat tggcgtactg
      661 gcgtaagtgg agaacgagca tgaaccttga agccttacca aaatattact ccccaaaatc
      721 tccaaaattg agtgatgccg caccggcgac aggctcaggt ggtttaacga ttacggatgt
      781 gatggctgcg caggggatgg tgcagtcgaa agcaccgctt gggtttgcct tattcctggc
      841 aaaagctggc gttcaggatc ctcagtttgc gattgaaggt ctgctcaatt acgcgatggc
      901 actggataac ccgacattga acaaattgag tgaagaaatc cggttacaga tcatccctta
      961 ccttgtgagt tttgcctttg ctgattattc caggtctgcg gcaagtaagg ctcgctgtga
     1021 gccttgtgct ggtactggat ttcataatgt attgcgcgaa gtggtgaaat actccagaag
     1081 cggggagtct gttatcaagg aagagtgggt gaaggaacta tgtcagcatt gtcatggtaa
     1141 gggagaagtc agcacagcgt gcagagggtg taagggtaaa ggtattgtcc tggatgaaaa
     1201 aaggacccgg cttcatggcg cgcctgttta taagatttgt gggcgttgca atggaaaccg
     1261 gtttagtcgt ttaccaacga cactggcgcg gtgtcatgtc cagaagctgg taccagacct
     1321 gactgattat cagtggtaca aaggatatgc agatgtcatt gataaactgg ttacaaagtg
     1381 ctggcaggaa gaagcatatg ctgaggtgca attaagaaaa gtgacgagat aaatgatttt
     1441 cgccgaagat ggcgacgtaa tgcttgcatt tttcaaaaaa tatggataaa attctttcaa
     1501 cgatgggctt tatatacccg acgttaagaa aaagtagaaa acccgctgat gagcgggttt
     1561 ttgtgcttta aatagggtaa tagagatgtt gaatctcatt ccgggattca tgtttgttta
     1621 cttattattt atcgggtgac tttgcctgat gtttaaaatg ttttcttcca gtgctatgtc
     1681 cctagataca atgagtctgc atattacatt attagcagag ctattacggt caaagtacag
     1741 cataagcttt taaagccaat caaccagtca tcaagacaga cggggttatt catgaaaact
     1801 ctccatgttt gattcgatgg ggcctgaaat taaagcttta atatagctca tgaaaggtaa
     1861 acattggcag ctgaagggcc acgcagacca tttatccggc aaaattccac gcgtaatccg
     1921 gtggtaattt cttctgcatc gcggagattg agcgctgaaa catgaagctg gacatcgata
     1981 cgaccatcgg atggggtgat aagacccttg ccgcttttgc cgtcaaaggt tttgacaatt
     2041 cctgtcattt tacgggacaa aaaaattcct taatactgat aactgggcgc actatacaca
     2101 cgttcctgaa gaaagctata gttttttgat ggggttgaag atggctggat gtctaaaata
     2161 aacattgctt catatgttca actatgcgtt aatgattgcg tcggtttgaa gaacagacga
     2221 tatacgaagt agtttactaa agcagttctc atttcaggtg ttattcactt attccttctt
     2281 tgagtctctc caattaagta cgaagtcgtt tctgttatac aagccattta tgccgaaagg
     2341 ctcaagttaa ggaatgtaga atgtcaaata aaatgactgg tttagtaaaa tggtttaacg
     2401 ctgataaagg ttttggcttt atttctcctg ttgatggtag taaagatgtg tttgtgcatt
     2461 tttctgcgat tcagaatgat aattttcgaa ccttatttga aggtcaaaag gttaccttct
     2521 ctgtagagag tggtgctaaa ggtcctgcag cagcaaatgt cataattact gattaaaatt
     2581 catagtttgt ctgtatacga taacgaagaa ggctgatgcc tgagtggaga tacagacaga
     2641 gtggtgaata ttggatctct ttaataaata gtaaggaggt ccaatacatg aaacaatggc
     2701 cagcatattt ggcaataact taatcaggaa aagtatgcta accattgtgg tgaagtgcag
     2761 gtttgctgca tgaatagttt tacagcagaa gctaactgct ggcatagcaa aacaaagtgc
     2821 gtaagtggat gactcccaca aaaagtacca caatattaaa cccgctcagg cgggtttttt
     2881 attatctgct ttaaatatgt tattaaaata taaaaaatac ttgttacgaa taaaatcaat
     2941 caagctacag ctttaagatt tgtctggaat actttgttgc aatgagggca gatcaaaagg
     3001 gcaccttttt gtactcttga aaaactgtgt tctgactctt gggtgcagtt tgggcaggaa
     3061 catttaacga gataattacg gcgtgatttt gagtctttac gttctgacat aggcttttcc
     3121 tgtataaatg gccgtataca gtacactaaa tatgaaaaca tatctcgtat tattatttaa
     3181 tatatgattt tcttttaaaa taattaccca catttttaat gtgtctgttt tttagcgccg
     3241 ttgagaacaa cgtttgctat aaaaactaac ccatagactc cgatcttttc aaacatattg
     3301 caccatctgt gtacatcggg gtgaggatat gaaatcaatg gataagttaa caacaggtat
     3361 cgcctatggc acatcggctg gtaatgctgg tttctgggca ttgcagttac tcgataaagt
     3421 aactccgtca cagtgggctg caatcggtgt gctgggtagt ctggtttttg gcctgctgac
     3481 gtatctgaca aacctttatt tcaagattaa agaagacagg cgtaaggttg cgagaagaga
     3541 gtaatccaat gacacaagac tatgaactgg ttgtgaaagg agtccgcaat tttgagaata
     3601 aagtggcggt aactgtagcc ttacgggaca aaaaacgctt tgacggtgaa atttttgacc
     3661 tggacatctc gctggaccgt gttgaaggtg ccgcgctgga gttttatgag gcagcagcca
     3721 gaatgagaat cagacaggta ttcctggatg ttgctgccgg gttatgtgta ggggatgagc
     3781 agtcgccgga aaagcgcccc ataattttag aggcgcagaa tgtgtggata acctacaaag
     3841 gaaagctacc gggaagaatt actggttctc tgaagacgcc accgacggca ttgcggtcag
     3901 aaaaagatga tattgaatcg cccattgaaa aactggaggg cagcgtcgct gatctgaata
     3961 aaaaattgtc ggtgctgatc ccttctgaag atgaaaaaaa acgccgcgat gagcagtttg
     4021 cggcgtttta cgattattgc attgaagtta ctcgcaggaa ttttgtgaag atttttgagg
     4081 agggtaaatc tcttcagtaa gcttaatggc ggacgctgca attaattcag gaaggtccgc
     4141 aaggtcatct gtcagtggat atgatgaaaa atcggcggca gttctgttaa gaagcgcttt
     4201 aactaattcc ttttccttct ccggcaacaa gttgattaga gctacgactg cttgcctgag
     4261 tgcgattaaa tcagcaaaag tttgttttgg tagatttgtg taatccatag tcacctctgt
     4321 gtttatcaga ttgacatcct cctcccgcca gtgcccatca ctggtgaggt aagatttaac
     4381 atatccgggg atttgaagcc gataaatcct gataaatatc catgaacgta aaaatcagat
     4441 acagcctgtc ggctgctgtt ctggcactga ttgccgccag tgctcctgcc cctgacattc
     4501 tcgatcagtt tctggatgaa aaagagggga atcacaccac tgcataccgc gatggctccg
     4561 gcatatggac catctgtcgg ggagcaacga tggtggacgg taaacctgtt attccgggaa
     4621 tgaaactgtc gaaggaaaaa tgcgcccagg ttaacgccat tgagcgggat aaggcgctgg
     4681 catgggtgga gcgcaatata aaagttccac tgaccgagcc acagaaagcc ggtatcgcgt
     4741 cattctgtcc ctataacatt ggccctggta agtgttttcc gtcgacgttt tataagcggc
     4801 ttaatgccgg tgatcgtaaa ggcgcatgcg aggcgattcg ctggtggata aaggacggtg
     4861 gacgtgattg ccgcattcgt tcaaataatt gttacggtca ggttattcgt cgtgaccagg
     4921 agagtgcatt aacctgctgg gggatagaac attgaatcag atattcacgg tgattttgct
     4981 cgtgttggta ggatttgtcg taggtaatgt ctggagcgac agaggatggc aaaaaaaatg
     5041 ggcggagcgt gatgctgccg aattatctca agaggtaaat gtccaatttg ctgctcgaat
     5101 aattgaacag gggcgaacta tatcccgtga tgaggctgtt aaagatgcac aacagaaagc
     5161 cgctgaaatt tctgccaggg ctgctgatct gtctgatagt gttaaccagc tgcgtgccga
     5221 agcaacaaaa tatgccatac gccttgacgc agcgcagcat accgcaaatc ttgccgctgc
     5281 cgtcagaggc aaaacaacca aagccgccga aggaatgctc accaacatgc tcggagatat
     5341 tgcagcagaa gctcagcttt atgctgaaat tgctgacaaa cgctacatcg caggagtga
//
LOCUS       DAZKHK010000220         5342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKHK010000220 DAZKHK010000000
VERSION     DAZKHK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.221"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            116..640
                     /gene="aroL"
                     /locus_tag="ACHOS5_004880"
     CDS             116..640
                     /gene="aroL"
                     /locus_tag="ACHOS5_004880"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP9779562.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            690..881
                     /gene="yaiA"
                     /locus_tag="ACHOS5_004881"
     CDS             690..881
                     /gene="yaiA"
                     /locus_tag="ACHOS5_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP9779563.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            1139..1816
                     /gene="aroM"
                     /locus_tag="ACHOS5_004882"
     CDS             1139..1816
                     /gene="aroM"
                     /locus_tag="ACHOS5_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP9779564.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            1888..2172
                     /gene="ppnP"
                     /locus_tag="ACHOS5_004883"
     CDS             1888..2172
                     /gene="ppnP"
                     /locus_tag="ACHOS5_004883"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP9779565.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            2329..2658
                     /locus_tag="ACHOS5_004884"
                     /pseudo
     CDS             2329..2658
                     /locus_tag="ACHOS5_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            2655..2735
                     /locus_tag="ACHOS5_004885"
     CDS             2655..2735
                     /locus_tag="ACHOS5_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779566.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(2816..3727)
                     /gene="rdgC"
                     /locus_tag="ACHOS5_004886"
     CDS             complement(2816..3727)
                     /gene="rdgC"
                     /locus_tag="ACHOS5_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP9779567.1"
                     /translation="MLWFKNLMVYRLSCEISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3852..4760
                     /gene="mak"
                     /locus_tag="ACHOS5_004887"
     CDS             3852..4760
                     /gene="mak"
                     /locus_tag="ACHOS5_004887"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP9779568.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4965..>5342)
                     /locus_tag="ACHOS5_004888"
     CDS             complement(4965..>5342)
                     /locus_tag="ACHOS5_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9779569.1"
                     /translation="ACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQAA
                     LADKGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQ
                     KRIKLSLGGLSPVEYRTEYQKAG"
BASE COUNT         1331 a         1295 c         1361 g         1355 t
ORIGIN      
        1 ccaccttaag tggaattttt tctttacaat cgaaattgta ctagtttgat ggtatgatcg
       61 ctattctcat gacaccggct ttcgccgcat tgcgacctat tggggaaaac ccacgatgac
      121 acaacctctt tttctgatcg ggcctcgggg ctgtggtaaa acaacggtcg gaatggccct
      181 tgccgattcg cttaaccgtc ggtttgtcga taccgaccag tggttgcaat cacagctcaa
      241 tatgacggtc gcggagatcg tcgaaaggga agagtgggcg ggattccgcg ccagagaaac
      301 ggcggcactg gaagcggtaa ctgcgccatc caccgttatc gccacgggcg gcggcattat
      361 tctgacggaa tttaatcgtc acttcatgca aaataacggg atcgtggttt atttgtgtgc
      421 gccagtatca gtcctggtta accgactgca agctgcaccg gaagaagatt tacggccaac
      481 cttaacggga aaaccactga gcgaagaagt tcaggaagtg ctggaagaac gcgatgcgct
      541 atatcgcgaa gttgcgcata ttatcatcga cgcaacaaac gaacccagcc aggtgatttc
      601 tgaaattcgc agcgctctgg cacagacgat caattgttga ttttcgagcg cctatactta
      661 acgttcatcc cgtgaaataa ggaagaacga tgccaacgaa accgccttat cctcgtgaag
      721 catatatagt gacgattgaa aaaggaaagc caggacagac ggtaacctgg taccaactca
      781 gagccgatca tcctaaacca gactcgttga tcagtgaaca tccgaccgct caggaagcga
      841 tggatgcgaa aaaacgctat gaggaccctg acaaagagtg accgcatcag actgctcgga
      901 agggattctg agtgccgcca caagggatct gcgtcacatt tttcacaatt catgtttttc
      961 aaataattag aatattaaac aataacaatc cattactgga atcatttgga atctttacat
     1021 tatgcagtgc aggtctgctg ctacgctttt tgtcatttgt agcacaagta agtgtcagca
     1081 gtggtgctta acacttgccc ggtaattaac gacgaaataa aagtaaggtg gaagaacaat
     1141 gagtgcgtcg ttggcgatcc tgaccattgg cattgtacct atgcaggaag ttttgccgct
     1201 cctgacagaa tacattgacg aagataatat ttcccatcat agcctgctgg ggaagttaag
     1261 ccgcgaagaa gtgatggcgg agtacgcgcc agaagcaggc gaagacacca ttctcacatt
     1321 attaaatgac aaccatctgg cccatgtttc gcgtcgcaaa gtggagcgag acctgcaagg
     1381 tgtggttgaa gtgctcgata atcagggtta cgacgtcatt atattaatga gtacagcaaa
     1441 cattagtagt atgaccgcgc gtaatacgat ctttcttgag ccgtcgcgga tattgccacc
     1501 gctggtttcc tctattgttg aagatcatca ggtgggggtt atcgtaccgg ttgaggagtt
     1561 gctgaccgtt caggcgcaaa aatggcaaat tttgcagaaa ccgccagtat tttcattggg
     1621 taatcccatt catgattcgg aacaaaaaat cattgatgcc gggaaagaat tactggcaaa
     1681 aggtgcagat gtcatcatgc tggattgttt gggatttaac cagcgtcatc gcgatttact
     1741 gcaaaaacag ctcgatgttc ctgtcttgct ctctaacgta ttgattgcac ggctggctgc
     1801 ggaattactg atgtaatttt gcgtgacagc cagcgcctct ggcccctata gtgaagtaga
     1861 tgttcaacta ccagacaggg ccagtttatg cttcaaagta atgagtactt ttccggcaaa
     1921 gtgaaatcga tcggcttttc cagcagcagc actggtcgcg ccagcgtggg tgttatggtt
     1981 gaaggtgaat acaccttcag caccgctgag cctgaagaga tgacggtaat cagtggcgcg
     2041 ctgaatgtgt tactgcctga cgcgaccgac tggcaagtgt atgaagccgg ttcggtgttt
     2101 aatgttcccg gtcacagcga gtttcatctg caagttgccg aacccacctc ttatctgtgc
     2161 cgctatctgt aattcctcgc cttccccttg aacgggaggg catttttctg aaatgtcctc
     2221 tctttaaccc ataataatat ttcctttgct gggatttttt cgatttccga tatattcata
     2281 attcatcaag gttgatataa atatcagcga agatctccag atactattgc ggaactggcg
     2341 acgttaaaag ataaatcaga tgatgaatgg tggtgtgcat tgctgcaaag tcgcaatgat
     2401 catttgcgcc aaacggcatt gcacaacgca catactccgg tgttgttaac gacattgact
     2461 gagcctcagg agcggtcgct ggctattaat aattcacagc tggctgcgga tgtgaaaacg
     2521 acgtggttaa aagaggagcc atcattactc ttatttgtcg aacaacccga tttttcgctg
     2581 ttacgtgatt tagtgaaaaa cggggcaacg cgaaaaattc gcagtgaagc gcgtcaccgg
     2641 cttgaggaaa aacaatgaca caacgcccct ggagcaagtt acagcataaa acgcataata
     2701 ttgctgcgct aagaattatt gcccgctgta gctaacgatt atattcctgg tgtggcttcg
     2761 tacgccggat aagacgcggc aggcgtcgca tccggcatta aaggtaaatc agcaattaac
     2821 gttgtgcttc gccacctaat ccttcaatca ggttttgaat taacgctgcc agttcaccag
     2881 tcatcaggat gaaatcggca tcaaaacgct gggcgaaatc ttcacggtcg atatcttcgt
     2941 tttgatcgcg cagctcgtcg cagaacttca gacgcttgag cgaaccatcg tcgcacatca
     3001 caaactgaat gcgctgctgc cagtcgagcg ccagtttagt caccactttt ccggcttcaa
     3061 tgtggttggt gatctcttcg ctggtcagat cttgtttctt cgcgcggatc acgccgccat
     3121 cttccagcaa cgatttcagc tcggcttcat caagcagttg gaaaccctgt gccgtgctgc
     3181 cggaacgtac ccattcggtc agcgtcagtt caatcgggtt ttccatgctc aatggtacaa
     3241 ccggtaacga ccccaggctt ttacgcagca gtgccagcgt atcttccgct tttttggcgc
     3301 tggcgcagtc caccataatc aaaccgttaa ccgtgtcgat ccacatcatt gtctggctaa
     3361 aacggctgaa agcacgcggc agcagagagt gcagcacttc gtctttcagc gaatcttttt
     3421 cggttttctt cagcttacgc gcctgttctg cttccagttt ggcgattttc gcttccagcg
     3481 cctgtttaat caccggggac gggaggattt tttcttcttt gcgcgcacag ataacaattt
     3541 gaccattggc aacgtgcgtt aacgcatcgc tgtgcgatcc catcggaggt acccagccca
     3601 tcttcgccat gtcctggctg ccgcatgggg taaatgccat cgaggctagc tgtttttcca
     3661 tctcttctgc acgcagcgaa atctcgcagc taagacggta aaccattaaa tttttgaacc
     3721 acagcatgat aatttccacg gccttgtcgt taaatttagc gggcatgata acgaattgtc
     3781 ggcggccttg cattgccaat ccggttgtcc gtctctacgc tattgatatt gaaaaaaata
     3841 aggagagtac cgtgcgtata ggtatcgatt taggcggcac caaaactgaa gtgattgcat
     3901 tgggcgatgc aggggagcag ttgtaccgcc atcgtctgcc cacgccgcgt gatgattacc
     3961 ggcagactat tgaaacgatc gccacgttgg ttgatatggc ggagcaggca acagggcagc
     4021 gcggaacggt aggtatgggc attccaggtt cgatttcgcc ctacaccggc gtggtgaaga
     4081 atgccaattc aacctggctc aacggtcagc cattcgataa agacttaagc gcgcggttgc
     4141 agcgggaagt gcggctggca aatgacgcca actgtctggc ggtttcagaa gcggtagatg
     4201 gcgcggcagc gggagcgcag acggtatttg ccgtgattat cggcacggga tgcggcgcgg
     4261 gcgtggcatt caatgggcgg gcgcatatcg gcggcaatgg cacggcaggc gagtggggac
     4321 acaatccgct accgtggatg gacgaagacg aactgcgtta tcgcgaggaa gtcccttgtt
     4381 attgcggtaa acaaggttgt attgaaacct ttatttcagg cacgggattc gcgacggatt
     4441 atcgtcgttt gagcggacat gcgctgaaag gcagtgaaat tatccgcctg gttgaagaaa
     4501 gcgatccggt agcggaactg gcattgcgtc gctacgagct gcggctggca aaatcgctgg
     4561 cacatgtcgt gaatattctc gatccggatg tgattgtcct gggcggcgga atgagcaatg
     4621 tagaccgttt atatcaaacg gttccacagt tgattaaaca atttgtcttc ggcggagaat
     4681 gtgaaacgcc ggtacgcaag gcgaaacatg gtgattcgag cggcgtacgc ggcgctgcat
     4741 ggttatggcc acaagagtaa aaaaaccagg caattggcgt atcatgcctg atgcgacgct
     4801 tgccgcgtct tatcaggcct acaaagtgag ccgtccggta ggccggataa ggcgctcgcg
     4861 ccgcatccgg caacaaataa cgcgcacagt ggcggcaacc cccggtgctc cgatgcctga
     4921 tgcgacactg gctgaactga accccaaaac ctggacagtt tcagttaacc ggctttttga
     4981 tactcagtcc ggtattctac cgggctgagt cccccaagac tgagttttat tcttttttga
     5041 ttccagtaag ttatgtattc gttaacggca ttttccagct cttctacact tctgtagtcc
     5101 cggcgatagt acatttcttc tttcaggtga ccaaagaaat tctccattac cgcattatca
     5161 agacagttgc ccttgcgcga catgctctgc actaaccctt tgtctgccag ggcagcctga
     5221 taacttttta tccggtaatg ccacccttga tcactgtgga gcagcggttt ttctccttct
     5281 gcaaggcttt ccagcccctt atttaacatc cgcttcacca gctcctctgt aggcctgcag
     5341 gc
//
LOCUS       DAZKHK010000221         5261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKHK010000221 DAZKHK010000000
VERSION     DAZKHK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.222"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..979)
                     /gene="druB"
                     /locus_tag="ACHOS5_004889"
     CDS             complement(<1..979)
                     /gene="druB"
                     /locus_tag="ACHOS5_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruB"
                     /protein_id="HFP9779570.1"
                     /translation="MNYAIDKFTGTLILAARATKYAQYVCPVCKKGVNLRKGKVIPPY
                     FAHLPGHGTSDCENFVPGNSIIIETIKTISKRYMDLRLLIPVGSNSREWSLELVLPTC
                     NLCRAKITLDVGGRSQTLDMRSMVKSRQIGAELSVKSYRIVSYSGEPDPKFVTEVERE
                     CPGLPSEGAAVFTALGRGASKGFPRAQELRCTETFAFLWRHPVAPDFPDELEIKSLAS
                     KQGWNLALVTIPEVPSVESISWLKSFTYLPVVPARTSITAIWPFLNQKTSINHVECVH
                     SDTILLSANMAPTSSENVGPTMYAQGSSLLLSAVGVEKSPAFFILNPGEN"
     gene            complement(1001..2215)
                     /gene="druA"
                     /locus_tag="ACHOS5_004890"
     CDS             complement(1001..2215)
                     /gene="druA"
                     /locus_tag="ACHOS5_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HFP9779571.1"
                     /translation="MHKDPSIIVNINLREAKLKKKVREHLQSLGFTRSDSGALQAPGN
                     TKDVIRALHSSQRAERIFANQKFITLRAAKLIKFFASGNEVIPDKISPVLERVKSGTW
                     QGDLFRLAALTWSVPVSSGFGRRLRYLVWDESNGKLIGLIAIGDPVFNLAVRDNFIGW
                     DTHARSSRLVNLMDAYVLGALPPYNALLGGKLIACLLRSRDLYDDFAKVYGDTVGVIS
                     QKKKQARLLAITTTSSMGRSSVYNRLKLDGIQYLKSIGYTGGWGHFHIPDSLFIELRD
                     YLRDMDHAYADHYMFGNGPNWRLRTTKAALNVLGFRDNLMKHGIQREVFISQLAENAT
                     SILQTGKGEPDLTSLLSAKEIAECAMARWMVPRSIRNPEYRLWKARDLFDFISNDSLK
                     FPPSDEIAKTVV"
     gene            2592..3563
                     /locus_tag="ACHOS5_004891"
     CDS             2592..3563
                     /locus_tag="ACHOS5_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISEc18 family transposase"
                     /protein_id="HFP9779572.1"
                     /translation="MNREIQQRLQWVKLYETSSDAGFVCRRCGISHPTLRKWWRRYLA
                     QGIAGLESHSRRPKHSPSTKTGAGEVALILELRSLRNLGARRIQSELKRLHAISLAVA
                     TIHKVLCQNKVKPVVKFRRKADFIRYERPVPGDRVQMDTCKIGPGLYQYTSVDDCTRS
                     RVLRLYSRRTAANTLDFIDCVIEEMPFPIQRLQTDRGREFFAVKVQEKLKEYSIKFRP
                     NKPASPHLNGKVERSQKTDKAEFYATVDLSTDDLKELLAEWQHYYNWERPHSAHNGKT
                     PMERYFELAEQTPYSDAVHASYQPNEEHIQEQNYKLELELRKLKRCL"
     gene            3573..5075
                     /locus_tag="ACHOS5_004892"
                     /pseudo
     CDS             3573..5075
                     /locus_tag="ACHOS5_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
BASE COUNT         1484 a         1202 c         1096 g         1479 t
ORIGIN      
        1 cattttctcc aggattaaga atgaagaaag caggtgattt ttcaacacca accgctgaaa
       61 gtaataaaga ggaaccttgt gcgtacatag ttggtccaac attttctgat gatgttggtg
      121 ccatatttgc tgacaacaat attgtgtcag aatgaacaca ttcgacatga ttaatacttg
      181 ttttttgatt taggaacggc caaattgctg taatagatgt tctggcagga acaacaggaa
      241 ggtatgtaaa agattttagc catgaaatac tctccacaga agggacttca ggaattgtaa
      301 caagagctaa attccatccc tgtttactag ctaaactttt tatttctaat tcatcaggaa
      361 aatctggagc aacagggtgt cgccaaagaa aggcaaatgt ttcagtacat cttaactctt
      421 gtgctcgtgg aaatcccttc gatgccccac gccctaaagc agtgaaaact gctgctccct
      481 cagaaggtaa accggggcat tctctttcaa cttctgttac aaattttgga tcgggttcac
      541 cactatatga aacaatacgg taagatttta ctgataattc agcaccaatc tggcgactct
      601 ttaccatact cctcatatca agcgtttggc ttctgcctcc tacatctaac gttatctttg
      661 ctctacataa attacaggtt ggcaacacta attctaatga ccactctcga ctattacttc
      721 cgacaggaat caataagcgc aaatccatat atcgctttga aatagtttta atagtttcga
      781 taatgatgga atttccggga acaaaatttt cacagtctga cgtaccatgt ccgggcaaat
      841 gagcaaaata tgggggtata accttccctt tacggaggtt aacacctttt ttacaaactg
      901 ggcaaacata ttgagcatat ttcgttgctc gagctgctaa tatcagtgtc ccggtgaact
      961 tatcaatagc gtaattcact tactccccct tcagttaaga ttagacaact gttttcgcta
     1021 tctcgtcaga cgggggaaat tttagcgagt cattactaat aaaatcaaat agatctcttg
     1081 ctttccaaag ccgatattct ggattgcgaa ttgatcgtgg aaccatccat cgtgccatcg
     1141 cacactcagc tatctcttta gcagaaagca aagaggttag atctggttca cctttgcctg
     1201 tttgcagaat actagttgca ttttctgcta gctgactgat aaaaacttca cgttgaattc
     1261 catgcttcat caaattatct ctaaatccta gtacatttaa agctgcctta gttgtacgta
     1321 aacgccagtt aggcccatta ccaaacatat aatgatctgc ataagcgtga tccatatcac
     1381 gtaagtaatc acgtaattca atgaacaagc tatcaggtat atgaaaatgc ccccaaccgc
     1441 ctgtatatcc aatcgatttt aaatattgaa ttccatccag ctttaaacgg ttatataccg
     1501 atgagcgccc catagacgat gttgttgtaa tagccaaaag acgtgcttgt ttcttttttt
     1561 gagatattac tccaacggta tcaccataga cctttgcaaa gtcatcataa agatcgcggc
     1621 tacgaagcag acatgcaatt aattttcctc ccagcagggc attataaggg ggaagagcac
     1681 cgaggacgta tgcatccatc aaattaacaa gccgggaact tctggcatga gtatcccacc
     1741 caataaaatt atctcggact gcaaggttga acacagggtc accaattgcg atcagcccta
     1801 tcaattttcc gttgctttca tcccatacaa gataccggag acgccttcca aatccgcttg
     1861 aaacaggtac ggaccaagtt aatgctgcta acctaaagag atctccttgc caggttcctg
     1921 actttacacg ttcaagtacc ggtgaaattt tatccggaat gacctcattg ccggatgcga
     1981 aaaatttaat aagctttgcc gctcttagcg ttatgaactt ttggtttgca aatatccgct
     2041 cagctcgttg agaactatga agagcccgta ttacatcttt ggtatttccc ggggcctgga
     2101 gcgctccaga atcagatctt gtaaaaccca aggattgtaa atgctcacgt accttctttt
     2161 tcagtttggc ttctcgaagg ttgatattaa ctattataga gggatctttg tgcattttta
     2221 tctcacgttc caattagtat ttagtcccac taaatttcac ttagagccca agtgattaat
     2281 cgcatggcac aagagagtcc cctccttgct ccctcgatcg ggacagcgcc aatgatagca
     2341 tttatacaag caattctggg agcttataga ttttgtgaaa aacttttgat agcaggaggt
     2401 tacaacagca ttgatgaaaa aaatcattca aatagattta tagattttgt taatgttatc
     2461 tgttttcaat ctaataacca cgaatatagc taattatagg atgattacta gttttactac
     2521 cctatcaacc aaacgttcac cggacattag atgtgtgatt catagacatc gtttcactat
     2581 aatgccttac tatgaacagg gaaattcagc agcggctaca gtgggtaaaa ctctatgaaa
     2641 cgtctagtga cgcggggttc gtatgccgtc gctgcggtat ttcccacccc acattgcgta
     2701 aatggtggcg cagatatctg gctcagggca tcgctggtct ggaaagccat agccgtcgcc
     2761 ctaaacattc accatcaacg aaaaccggcg ctggcgaagt cgctctgatt ctggagttac
     2821 gttccctgcg aaatttgggt gcaaggagga ttcagagcga gttaaaacga cttcatgcaa
     2881 tctcactggc agtggcaacg atccacaaag ttctttgcca gaataaggtt aaacctgtcg
     2941 ttaaattccg gcgtaaagca gattttattc gctacgaacg ccctgttccc ggtgatcggg
     3001 tccagatgga tacctgtaaa attggccccg gtttgtatca gtacacatct gttgatgact
     3061 gcactcgctc cagagtcctg agactctact ctcgtcgcac ggctgcaaac acactggatt
     3121 ttatcgattg tgttattgaa gaaatgcctt tcccgataca gcgcttacag acagaccggg
     3181 gaagagagtt ttttgccgtt aaggtgcagg agaaattgaa agaatacagc atcaagtttc
     3241 gtccaaataa gccagcctca cctcatctta atggcaaagt tgaacgttct caaaagacag
     3301 acaaagccga gttttacgct accgtagacc tctcaactga tgacctgaaa gaactgctgg
     3361 cagaatggca gcattactat aactgggaac gccctcatag cgctcacaac ggtaaaactc
     3421 cgatggagcg ctacttcgaa cttgcagagc aaacaccata ctctgatgcc gttcacgcaa
     3481 gctatcagcc caatgaagag catatacagg aacagaatta caagcttgaa cttgaactga
     3541 gaaaattgaa acgatgtcta tgaatcacac agatgaatat cagaaaactg ttttgtccgg
     3601 gccccccccc gggttttatt gtttttattc ttttttgttg tttctgtcat aaccacaatt
     3661 gcatacggat acactgagaa gaatgccacc gggaatgtgt tgctgctgtt ccttcttctg
     3721 cttcttgcac acagaaatac cctcacatcc attacagctc tctgttattt ctgttctgtt
     3781 gtgcactgta tgcgcctgcc ggtatgacgt acggtaaaat caacaacagt cttattgtcg
     3841 cgttgttgca gaccacaact gatgaggcag cggagtttac cgggatgatt cctatttatc
     3901 attttctggt cagtgccgcg attctggtat tcatggtgat tttctggcga acacaccacc
     3961 gcggtcaccg taactggctg gcactgctgc tattcgtatt atgctcagta aacagttggc
     4021 cgttgcagat agttaaagga actgttgtgg ggacaactga cacattgcgt gaaatgcagc
     4081 attataaaca actgagtcag cacggggctg acaactggaa aatcctgccg ggaacaccgt
     4141 tatatgacac gattgttatt gtcactggtg agagtgtgag cagggattat atgtcggtat
     4201 atggttatcc cgtgccaacc acaccgtggc tgaatacggc tcccggttta tttattgacg
     4261 gctatacatc gacagcagcc agcactgtgc cctccctgag ccggacactg atttatgact
     4321 atgagcagaa ccctgattcc ggcaacaacg tggtggcgct ggcagcaaaa gcaggataca
     4381 gcacatggtg gatatccaat cagggaaaac tgggagagca tgacacacgc atctctgtta
     4441 ttgcttctga tgcagagcat accgttttcc tcaagaaagg cagctttgct tcccgtaaaa
     4501 cagatgacat gcagttgtta caggaaacag aacgtgcgct ggcggataca tcctctccga
     4561 aggtcatttt cctgcacatg atgggttctc atccgaatcc gtgtgaccgg cttaactcct
     4621 ggccgaataa ttaccaggag cagttcccac ggaagattgc ctgttacctc gccagcatca
     4681 gtaaactgga taactttctc gggcagcttg atggtatcct tcgccggcat tcccgtcatt
     4741 ttgccatgct ttacttttct gaccatggac tgtcagtcag cgacagtact aatcctgttc
     4801 atcatgatgg ccatgtgcag gctggctaca gtgttcccct gattattacc gccagtgaca
     4861 tcacatctca ccagtccgtc agcagaaaaa tcagtgcccg tcatttcgca ggcatttttc
     4921 agtggctgac tggtattcgt actgaaaata taccgccatt caacccgctg ataaatgaag
     4981 ataatgaacc tgttatggtt tttaacggag agagaatagt tcctgcggac ggccttaagt
     5041 cacagcctgt gattcttccg ggcaagggaa aataagagta atgttgagca ccatgttctg
     5101 tacgatgaat tttccactgt ttatatcgat tattttgcta ataaccagag ttgagcaccc
     5161 gaaaatacct gaagaggaat tacccgctac caggaggttc agatatccct gatgtcggct
     5221 ataaaaattt tgattacatg acgtaatgaa agtggctctg t
//
LOCUS       DAZKHK010000222         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKHK010000222 DAZKHK010000000
VERSION     DAZKHK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.223"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1634
                     /gene="sapA"
                     /locus_tag="ACHOS5_004893"
     CDS             <1..1634
                     /gene="sapA"
                     /locus_tag="ACHOS5_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP9779573.1"
                     /translation="LSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFN
                     PSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPF
                     QKTDWFTPTRKMNADDVVFTFQRIFGRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLD
                     NHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPYQLSE
                     YRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSI
                     LRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAET
                     AASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKT
                     AELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSC
                     AAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLASSLR
                     LQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            1631..2596
                     /gene="sapB"
                     /locus_tag="ACHOS5_004894"
     CDS             1631..2596
                     /gene="sapB"
                     /locus_tag="ACHOS5_004894"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP9779574.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIISAIRHMVLPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(2738..>5189)
                     /locus_tag="ACHOS5_004895"
     CDS             complement(2738..>5189)
                     /locus_tag="ACHOS5_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP9779575.1"
                     /translation="ERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRL
                     AGIIDDMLARRREIIREAGLEESGVVDAWQKAYRYYVPLKGQDVDGVVSLPRTGKGFT
                     IGGRESKQAMGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFLKLVQDNPDKDYWQV
                     FTNERPDTMRAIAERVDPETGETRREVVERPVPMAMMADRYFTTKKDGKTYYIKLHDP
                     RLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAE
                     QGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGG
                     KTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSA
                     YKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGH
                     LNGDGPLLERLRWKNLNVTQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHV
                     KERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGG
                     VLGAFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENIPFGTPKP
                     DSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTGRFISK
                     TTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGA
                     ERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSSLSLAQQAAIVKSVERDM
                     KVAVDRFNREYNKKVGVE"
BASE COUNT         1112 a         1456 c         1276 g         1345 t
ORIGIN      
        1 tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg
       61 aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag
      121 tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc
      181 agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg
      241 ccgaaagctg ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg
      301 ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gatgatgtgg
      361 tgtttacctt ccaacggatt tttggccgca acaacccgtg gcataacgtc aacggcagta
      421 acttccccta tttcgacagt ctgcaatttg ccgataacgt gaaaagcgtc cgcaagctgg
      481 ataatcatac cgttgagttc cgtctcgccc agccggatgc ttcttttttg tggcacctgg
      541 cgacccatta tgcttcggtc atgtcggcag aatatgcagg gaagttagag aaagaagatc
      601 gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc
      661 gcgccgggca atatattcgc ttacaacgtc atgatgattt ctggcgtggt aagccgttga
      721 tgccacaagt ggtggtggat ttaggctctg gcggtacagg acgattgtcg aaactcctga
      781 ccggggagtg cgacgttctg gcctggcctg ctgccagtca gctatccatt ttgcgtgacg
      841 acccacgctt gcgtttaacg ctgcgcccgg ggatgaacgt cgcctatctg gcgtttaaca
      901 ccgctaaacc gccgctaaat aatcccgctg tccgccatgc gctggcactg gcaattaata
      961 accagcgcct gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac
     1021 cgcgagcctc gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcaaaat
     1081 cgcgcgaaca gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca
     1141 cacgttcgca ggcgtggaac cccagtccac tgaaaaccgc cgaactgatt caggcggata
     1201 tggcacaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgtttt caggaggcgc
     1261 ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc
     1321 cggacagttt cttccgtccg ttactgagct gcgcagcaat tcattcgcag accaacctcg
     1381 cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctct tcgcagcagc
     1441 tggcggcgcg tattgaagcc tatgacgaag cgcaaagtat tctggcgcag gaattaccta
     1501 ttctgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg
     1561 tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgaaaaa caggatgagg
     1621 tgaaaaagcc atgattatct tcaccttacg ccgtattttg ttattgattg tgaccttgtt
     1681 cctgctgacc ttcgttggct tcagcttgag ctatttcacc ccacatgcgc cgttacaagg
     1741 agcgtcactg tggaatgcct gggtgttctg gtttaacggc ctgatccact gggattttgg
     1801 tgtttccagc attaacggtc aaccgattgc cgaacagtta aaagaggtct tcccggcgac
     1861 gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtaggaat
     1921 gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact
     1981 gggcttttca atcccggtgt tctggctggc gcttctgctg acgctgtttt gttcactcac
     2041 actaggttgg ttgcccgttt ctggtcgttt cgatttgctc tacgaagtga aaccgattac
     2101 cggttttgcg ttgattgatg cctggctttc ggactcaccg tggcgggatg aaatgatcat
     2161 tagcgcaatc cgtcatatgg tattgcccgt gatcaccctg tcggttgcgc caacaactga
     2221 agtgatccga ctaatgcgta tcagcactat cgaagtgtat gaccagaatt atgtaaaagc
     2281 ggcggcgacc cgcggtttgt cacgctttac tattttgcgt cgccacgttc tgcataacgc
     2341 gttacctccg gttattcctc gcctgggctt acagttttct accatgctga cgctggcgat
     2401 gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg
     2461 ccagcaggat tacgctgcca tttccgccgg agtgatggtg tgtggctcac tggtcattat
     2521 tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg
     2581 gtatgcctta cgatagtaat tataattaga gacaaaacaa taataaaatc aatgcattgt
     2641 caataaaacc atcactaaca tcttgttttt atatacacaa atatatacac attgattgta
     2701 ctgttatgcc ccgtacaaaa gtacggggca tgttctgtta ctccactccc acttttttgt
     2761 tgtactcgcg gttaaagcga tccacggcaa ccttcatatc ccgctctacc gatttcacta
     2821 tcgccgcctg ctgcgccaga ctgagagaac tgtcggcata aatggcatca cgctgtttac
     2881 gcaaatcctt cagtctcttc cgggtatcct gcataagccc gttcatcgac aattttccgt
     2941 tgttctcgtc aatgaacgcc gttctttctg caccggtcag actcttcagc tctgcgtgat
     3001 actgcgcgac ctccgtcatc cggtcgtaca tcttctgctg gtcagcatac ggcatcacct
     3061 cacctgaaat tttccccagg aagggcacct gctgttccgg tatatcaata ccattcagcg
     3121 atttcaccgc cgcatccgtg gttttggaaa tgaagcgccc tgtccctccg gagatatagt
     3181 caatccagaa tttcagcgat tccggtgtga tatccaccgc gccgggacga tactggctgc
     3241 cacctgagaa cgcattcagc caggatgcaa acgccttgta cgcttctggc gttgaacgtc
     3301 ttcccagctg gctgtcaggt tttggtgtac caaacggtat gttctcctgg taaatctgcg
     3361 cccccatgaa gttttcattc atggcaaggt tcgcaaacgg acgcagaatg gtcggcgctg
     3421 catttttcag caatgccccg gacagtgttt ccgacgtttc actgcccaca ggactgaatg
     3481 ccccaagcac accaccaaca acattaccgg cagcacgtga agcggtcagg tcgcccgctg
     3541 ccaccccttc ggctgtgtga ccaagcagga agaaaacgtt gtacccgtaa ggcagaggaa
     3601 tactccagta ctctccggcc ttgcccccga acaccgattt cataatgacg aggttacgct
     3661 ctttcacatg agacggcacc ttgtcatacc agttaacccc gtcatcatcc tcccccgcaa
     3721 cactgcggtt aagcgagcca agcagataac ccgctcccac agctgcaagc gcgattttct
     3781 gcgttacatt gagattcttc cagcgaaggc gctccagtaa cggcccgtcg ccattaagat
     3841 gtccgagcgt tctcaccaag tttgcggtcc cctgaatgct ggcgttggca aacatataca
     3901 gcgagttcat cagcgctccc tgctcaccac gacggttaaa gttcaccgtc atgtttttgg
     3961 caagagacgc cgcctgctgg cgtgacaaac cggcatcacg agcgtgctta taggcagaaa
     4021 gacgcagtgc gttttcaacc gcaccgttgg catcctctac aaggttcagg aacgaattcc
     4081 atgcaccgat actctggcct ttccatcctc ccttcgccag cgatacaagg cgatccattt
     4141 ccttctgctg gccttcaagg tcacccatgt taaaccagcc ggttttccct ccgtcctcaa
     4201 caaactcttt ccacaccttc tgccactgcg caccgttgcc cgtgagggtt ttaccacgca
     4261 gactggcgta tacggcagac atggcagaac ggctgtcttt caccacagcc agtgcggata
     4321 agttatccag ccctttcagt ttgccgtcac tccttccctg ctcagccttc aggttcatca
     4381 ccgccgtctg cacgtcacgg atgaagttac tgaccagaaa ttccgggtta tacgacgtgt
     4441 tcaccgttgc caggaagcgg ttaactttcc ccagcgtacg gattacggcg ttactggttt
     4501 ccggccccat gttcttcatc gcacgcatca ggcgcggatc atgaagttta atgtagtaag
     4561 ttttgccgtc ctttttggtg gtgaagtagc gatccgccat cattgccatc ggcacaggac
     4621 gttcgacaac ttcgcgacgg gtttcgccgg tttccggatc aacacgctct gcaatagcac
     4681 gcatggtgtc cggtcgctca ttggtgaata cctgccagta atccttgtcg ggattatcct
     4741 gtaccagttt caggaaggcg ttacccactt catttttgcg attgcggatc agcgattcgc
     4801 tcaaatcctg tatcgcctga gtggaaggag actgtgcgcg ggatgcacgc cccatggcct
     4861 gcttactttc acgcccgccg atggtgaatc ccttgcctgt acgtggcagt gacaccacac
     4921 cgtcaacatc ctgccctttc aggggaacgt agtaacggta ggctttctgc caggcatcca
     4981 ccacgccgct ctcttccagt cctgcctcac ggataatctc acggcgacgg gccagcatat
     5041 cgtcaataat ccctgccaga cggtcatact gtgcctgttt gccgctgtta cgcacacgct
     5101 gcatgatttc cgccgcttcc gcgttggtca tccccgaacc gccgtccggc attttagggt
     5161 tgattttcgc aatatgtgcg ttacgttcc
//
LOCUS       DAZKHK010000223         5184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKHK010000223 DAZKHK010000000
VERSION     DAZKHK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.224"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..696
                     /locus_tag="ACHOS5_004896"
     CDS             97..696
                     /locus_tag="ACHOS5_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9779576.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDARHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            761..3787
                     /locus_tag="ACHOS5_004897"
     CDS             761..3787
                     /locus_tag="ACHOS5_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9779577.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSNTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSASEAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEAEKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSSASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASARATAAGKSAESAASSA
                     STATTKAGEAAEQASAAARSASAAKTSETNAKASETSAESSKMAAASSASSAASSASS
                     ASASKDEASRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSTGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLSSKGRVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASVSSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            3787..4362
                     /locus_tag="ACHOS5_004898"
     CDS             3787..4362
                     /locus_tag="ACHOS5_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9779578.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTSQDIEWPALP"
     gene            complement(4460..5050)
                     /locus_tag="ACHOS5_004899"
     CDS             complement(4460..5050)
                     /locus_tag="ACHOS5_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9779579.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         1372 a         1297 c         1401 g         1114 t
ORIGIN      
        1 aaatccacag caggtatcag tatcagctga attttataac ccagaacggg cgtcagaaat
       61 gacgcctttt ttattgcaga aaagcgagag gtaattatgc gtaaacttta tgcagccatt
      121 ttgtccgcag ccatttgtct ggccgtatcc ggtgcgcctg catgggcgtc tgaacatcag
      181 tccacgctga gcgcggggta tcttcatgcc cggacgaacg ttcccggcag tgatgatctg
      241 aacgggatta acgtgaaata ccgttatgag tttacggaca cactggggat ggtgacgtcg
      301 ttcagctatg caggagacaa gaatcgccag ctgacccatt acagcgatac ccgctggcat
      361 gaagattccg tgcgtaaccg ctggttcagc gtgatggcgg ggccgtctgt acgcgtgaat
      421 gaatggttca gcgcgtatgc gatggcaggc gtggcttaca gccgtgtgtc gactttttcc
      481 ggggattatc tccgcgtaac tgacaacaag ggaaaaacgc acgacgtgct gaccggaagt
      541 gatgacgctc gccacagtaa cacctctctg gcgtggggag ctggcgtgca gtttaacccg
      601 accgaatccg tggccgttga tgtcgcttat gaaggctccg gcagtggcga ctggcgcact
      661 gacgggttca tcgttggtgt tggttataaa ttctgattag ccaggtaaca cagtgttatg
      721 acagcccgcc ggttcaggcg ggcttttttg tggggtgaat atggcagtaa agatttcagg
      781 tgtactgaaa gacggcacag gaaaaccggt acagaactgc acaatccagc tgaaagcaaa
      841 acgtaacagc aacacggtgg tggtgaacac gctggcctca gaaaatccgg atgaagccgg
      901 gcgttacagc atggacgttg agtacggtca gtacagcgtt attctgttgg tggagggatt
      961 cccgccgtca catgccggga ctatcaccgt gtatgaagat tcccgacccg gtacgctgaa
     1021 tgattttctc ggtgccatga cggaggatga tgcccgtccg gaggctctgc gccgttttga
     1081 actgatggtg gaagaggtgg cgcgtaacgc gtccgcggtg gcacagaaca cggcagccgc
     1141 gaagaagtca gccggcgatg ccggcacatc tgccagtgag gcggcaaccc atgcgactga
     1201 tgctgcaggc tcagcacgcg cagccagcac gtcagccgga caggccgctt cgtcggctca
     1261 gtcagcgtcc tccagcgcag gaacggcatc aacaaaggcc actgaagcgg aaaaaagtgc
     1321 tgccgctgca gagtcctcaa aaagcgcggc ggctaccagt gccggtgcgg cgaaaacgtc
     1381 agaaacgaat gccgcagcgt cacaacaatc agcagccact tctgcatcca ccgcgactac
     1441 gaaagcgtca gaagctgcca cctcagcccg ggatgcggcg gcctcaaaag aggcagcgaa
     1501 atcatcagaa acgaacgcat cctcgagcgc cagtagttcc gcttcctcgg caacggcggc
     1561 agcaaattcc gcgaaggcgg caaaaacgtc cgagacgaac gccaggtctt ctgaaacggc
     1621 agcgggacag agtgcctcag ctgcggcagg ctcaaaaaca gcggctgcat catctgccag
     1681 tgccgcgtca acaagtgccg ggcaggcctc agccagagcc actgccgccg gaaaatcggc
     1741 agaaagtgcc gcatcgtctg cttcaacagc cacaacgaag gctggcgaag ccgctgaaca
     1801 ggccagcgca gcagcgaggt ctgcatccgc agcgaagaca tccgaaacga acgcgaaagc
     1861 ctcggaaacc agtgcagaat cctcaaaaat ggcagctgca tcgtccgcca gttcggcggc
     1921 gtcatcggca tcatctgcgt ctgcttcaaa agatgaggcg agcagacagg catcagcagc
     1981 gaaaggcagc gccacgacgg catccacgaa ggcgacagag gcagctggca gtgcgacggc
     2041 agcagctcag agcaaaagta cggcggaatc tgcagcaacg cgcgctgaga cagcggcaaa
     2101 acgggcagag gatattgcat ccgccgtggc gcttgaggat gcgagcacga cgaaaaaggg
     2161 gatagtacag ctcagcagtg cgaccaacag cacttccgag tcactggcgg caacgccaaa
     2221 agccgttaag gccgcgtatg acctggctaa cgggaaatac accgcacagg atgcaacgac
     2281 agcacagaaa gggataatcc agctaagcag cgcgaccaac agcacgtctg aaacgctggc
     2341 ggcaacgcca aaggcagtaa aagcagccaa tgacaatgct gagaaacgtc tgcagaaaga
     2401 tcagaacggt gcggatatcc ctggcaaaga cacctttacg aaaaatattg gtgcctgccg
     2461 tgccttcggt gggtcagtaa gcacaacaac aggaaactgg acgactgcac agtttatcga
     2521 gtggctggat tctcagggag catttaacca tccatactgg atgtgcaagg gttcctggtc
     2581 ttatggcaat aataaaatca ttactgatac tggctgcggt aatattcatc tcgccggagc
     2641 tgtcattgaa gtaatgggga taaagtcagc gatgacgatc cgcattacca caccgaccac
     2701 ctccacgggt ggtggaacaa ctaacgccca gtttacctat attaatcacg gaacagatta
     2761 ttcacctggc tggcgaaggg actataactc cagaaataag ccaacggcat cagagatcgg
     2821 ggcgttaccg tcaggtggaa cagcagtatc atcagttaat ctgtcttcaa aaggtcgggt
     2881 aaccgcgctg acagacaata cgcagggggc aacaggtctt gagttatacg aggtgtataa
     2941 caacggatat ccaacagcgt atggaaatat cattcacctg aaagggatga cagccgttgg
     3001 cgaaggtgag ttactcatcg gctggagtgg tacaagcggt gctcatgctc cggcatttat
     3061 tcgttcacga cgggatacga ccgacgcaaa ctggtcgccg tgggcgcagc tttacacctc
     3121 ggctcatcct cctgcagagt tttatccagt cggcgcacca atcccgtggc catcagatac
     3181 cgttccgtct ggttatgccc tgatgcaggg gcagactttt gacaaatctg cttacccgaa
     3241 acttgcagtc gcttatccgt caggcgtgat ccctgatatg cgtggctgga cgattaaggg
     3301 caaacctgcc agtggtcggg ccgtattgtc tcaggaacag gacggcatta aatcgcacac
     3361 ccacagcgcc agcgtatcca gtacggattt ggggacgaaa accacatcgt cgtttgatta
     3421 cggcactaaa tccacgaata acaccggggc gcatacgcac agtgtgagcg gtacagccgc
     3481 aagtgccgga aaccatactc atagtgtcac aggcgcatca gcagtcagcc agtggtcaca
     3541 aaatgggtca gtacataagg tagtgtctgc ggccagtgtg aatacaagtg ctgcaggagc
     3601 acacactcat agtgtcagcg gcacagctgc atctgcaggt gctcacgcac atactgtcgg
     3661 tattggtgct catacgcact ctgttgcgat tggctcacat ggacacacta tcaccgttaa
     3721 cgctgctggt aacgcggaaa acaccgtcaa aaacatcgca tttaactaca ttgtgaggct
     3781 tgcataatgg cattcagaat gagtgaacaa ccacggacca taaaaattta taatctgctg
     3841 gccggaacta atgaatttat tggtgaaggt gacgcatata ttccgcctca tacaggtctg
     3901 ccagcaaaca gtaccgatat tgcaccacca gatattcctg ctggctttgt ggctgttttc
     3961 aacagtgatg aggcatcgtg gcatctcgtt gaagaccatc ggggtaaaac ggtttatgac
     4021 gtagcgtcag gggacgcgtt atttatttct gaactcggtc cgttaccgga aaatgttacc
     4081 tggttatcgc cggaagggga gtttcagaag tggaacggca cagcctgggt gaaagatgca
     4141 gaagcagaaa aactgttccg gatccgagag gcggaagaaa caaaaaacag cctgatgcag
     4201 gtagccagtg agcatattgc gccgcttcag gatgctgcag atctggaaat tgcaacggag
     4261 gaagaaatct cgttgctgga agcatggaaa aagtatcggg tattgctgaa ccgtgttgat
     4321 acgtcaactt cacaggatat tgaatggcca gcactgccgt agggtaaaac atataaattc
     4381 tataattaga tgtatctttc catttacggc aaggaagggg gcttggaaga cgtaaagcat
     4441 ctcacaccaa gattattttt tatatgtcag gtgtctgaag ttttgctttg gctcttaaaa
     4501 tggtttgccg cgaggttttg aattcccggg caatggcact tatacttaca cctgacttaa
     4561 ttcgttcgaa taccacctgt ttctgttctt catttaacac aggtggtcga ccaaaacgtt
     4621 tccctgcgcc ccgggctctt actatcccgg aatgagtgcg ttcaagtaaa aggtctcgtt
     4681 caaattcagc gactgctgaa attacttgca tcatcatttt ccctgttgga ctggtcaggt
     4741 caatgccccc caatgctaag caatgcactc tgatacctgt ttcggtcagt tgttccactg
     4801 ttttcctgat atccattgca ttacaaccaa ggcgatccag ttttgtcaca atcaattgat
     4861 caccacattt cagacgagca agcaaccggt taaaaccagg acgctcactg gttgctgctg
     4921 agccgctaat gtgttcttcg attatttgct gaggtttgat tttaaaacct gcactttcga
     4981 tttcccggcg ttgattttcg gtggtctgat ccagcgttga tatccgacag taagcaaaaa
     5041 ttcgagacat agtgagactc tatacgaaat tggtgtacat atcataatgc atatccaaaa
     5101 attatttcgc ttattattgt acatatttgt atgtatacgt tcgaaaataa acgaattcgg
     5161 atgcagtccc gtaattttgg tgta
//
LOCUS       DAZKHK010000224         5157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKHK010000224 DAZKHK010000000
VERSION     DAZKHK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.225"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(270..515)
                     /locus_tag="ACHOS5_004900"
     CDS             complement(270..515)
                     /locus_tag="ACHOS5_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779580.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            779..2872
                     /gene="cda"
                     /locus_tag="ACHOS5_004901"
     CDS             779..2872
                     /gene="cda"
                     /locus_tag="ACHOS5_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFP9779581.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2869..3132
                     /locus_tag="ACHOS5_004902"
     CDS             2869..3132
                     /locus_tag="ACHOS5_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFP9779582.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            3202..3348
                     /locus_tag="ACHOS5_004903"
     CDS             3202..3348
                     /locus_tag="ACHOS5_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP9779583.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
     gene            4055..4243
                     /locus_tag="ACHOS5_004904"
     CDS             4055..4243
                     /locus_tag="ACHOS5_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779584.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTILTDAT"
     gene            complement(4395..4586)
                     /locus_tag="ACHOS5_004905"
     CDS             complement(4395..4586)
                     /locus_tag="ACHOS5_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP9779585.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4654..4977
                     /locus_tag="ACHOS5_004906"
     CDS             4654..4977
                     /locus_tag="ACHOS5_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9779586.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            4967..>5157
                     /locus_tag="ACHOS5_004907"
     CDS             4967..>5157
                     /locus_tag="ACHOS5_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFP9779587.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELI
                     DATPFAKKYTSGVLSFAEQT"
BASE COUNT         1262 a         1082 c         1426 g         1387 t
ORIGIN      
        1 tcgcgctcat tcctcagcct catcccgcga tgtctgcgta tgtcatcaac tgaaaagatg
       61 gaaaaaaaac agtgtcatga ggtttacgca ggcaaaacca gcgttattca catggctgaa
      121 tagctatgca tacggttaaa atttatcagg tgcgatcgcg gcaacttttc gggtggtttg
      181 ttgccatttt tacctgtcag cagccgtgat cgcgctgaac gcatttcagc ggtgcgtaca
      241 ataaggggtt atgttaaata acccttagct tagcggcggc gcagtggttg ccaggtgaag
      301 gtgtcatccg ggtcctgtcc ggcagtcagt gtggcttctc gatttttgct ggccgttcgt
      361 tcatcaagaa tggctttaag tatccgtgcc cggaggcgtc gctttgtttc ctgcggcatt
      421 ttgtcctttt tcgtcagccc catggcacga gcttcagcgg caatggcttc tgccagtcgg
      481 cgctcttcgg cttcggtcag tctatggcgt ttcatggtgg tctccgggtg tttctctggt
      541 gtcataacag tttaactgtt ggctctctcc ttatgctaat cagccttctt tcatgcctgt
      601 tgccattgag tttaagaatg attggttgtt tttcttgagc gttttcacaa aaacgtaaat
      661 ccagtgggtt gacaggattt acgaggtgac gtaacttttt actgtacata aaaccagtgg
      721 ttttatgtac agtttgttgt ttttaacgca ttgttttaaa agtcaaagag gtgtttttat
      781 gagtgattac gaaggtagtg gtccgacaga aggtattgat tatgggcact cgatggtcgt
      841 gtggccgtca acaggactga tttcgggtgg tgatgtgaaa ccaggaggct catcaggtat
      901 cgctccatcc atgcctccgg gatgggggga ttacagccca cagggtatcg cacttgtaca
      961 aagtgttctt tttccgggaa ttattcgccg gattattctg gataaggaac ttgaagaggg
     1021 agactggtcg ggatggtctg tcagtgtgca tagcccttgg ggaaacgaga aagtttccgc
     1081 tgcacgaaca gttcttgaga atggtttacg tggtggtttg ccagaaccgt ctcgcccggc
     1141 tgctgtttct tttgcccgtc tggagcctgc ttccggaaat gagcaaaaaa ttattcgtct
     1201 tatggttaca cagcaactgg aacaggtaac ggatattccg gccagccagt taccagcagc
     1261 gggtaataat gtaccggtga aatatcgtct gatggacctt atgcagaacg gtacgcaata
     1321 tatggctatt atcggaggta ttccgatgac agtgccggta gttgatgccg ttccagttcc
     1381 ggaccggagt cgtccgggaa caaatattaa agatgtttac agtgcccctg tatcaccaaa
     1441 tctaccggac ctggtattaa gtgtgggtca gatgaatact ccggttctgt ctaatcccga
     1501 aattcaggaa gaaggagtta ttgctgagac aggtaattat gttgaggctg gttatacgat
     1561 gtccagtaat aatcatgatg tcattgttcg ttttcctgat ggcagtggtg tttctccgtt
     1621 atatatttca actgtagaga ttctggacag taatggtctg agtcagcgtc aggaagccga
     1681 aaataaagca aaggatgatt tcagagtcaa gaaagaagaa gcggtagccc gggctgaagc
     1741 tgaaaaagca aaagccgagt tatttagtaa agcaggtgtg aaccagcctc ctgtatatac
     1801 acaggaaatg atggaaaggg ctaattcagt aatgaatgag cagggagctc ttgttctgaa
     1861 taacactgcc agttctgtac aactggcgat gaccggaact ggtgtctgga ctgctgctgg
     1921 tgatattgca ggcaacataa gcaagttttt cagtaatgct ctggagaaag taaccatacc
     1981 cgaagtgagt cccctgctta tgcggatttc tcttggtgct ctgtggtttc attcagaaga
     2041 agctggagca ggaagtgata tcgtgccggg gcgaaatctg gaggcgatgt tttcgctgag
     2101 tgctcagatg ttggctggac agggcgtggt cattgaacct ggtgcgacga gtgtaaatct
     2161 gcctgttcgt ggacaattga taaacagtaa cgggcaatta gctctggatt tactgaaaac
     2221 agggaatgaa agtatcccag ctgcggttcc tgttcttaat gctgtccgtg atacagcaac
     2281 aggactggat aaaatcacgt taccagcagt agtaggcgca ccttcccgga cgattctggt
     2341 taatccggta ccacaacctt cggtgccaac agatacaggt aatcatcaac ccgttccggt
     2401 tacaccagtg catacaggaa cggaagtaaa accggtcgaa atgccagtaa cgacgattac
     2461 tcctgtttct gatgttggtg gactacgaga ttttatttac tggcgtcctg atgctgctgg
     2521 gactggtgtt gaagctgttt atgtgatgct taatgatcct ctggattcag ggcgattttc
     2581 gcgtaaacaa cttgataaaa aatataaaca tgctggtgat tttggtatta gtgatacaaa
     2641 aaagaatcgt gaaactctta ctaaattcag ggatgctatt gaggagcatt tatcggataa
     2701 ggatacagta gagaaaggaa cataccgaag agaaaaaggt tcaaaagttt attttaatcc
     2761 taatacgatg aatgtggtta taattaagtc aaatggtgag ttcttatctg ggtggaaaat
     2821 aaatccagat gcggataatg gtcgaattta tttagagacg ggtgaactat gaacaagatg
     2881 gcaatgatcg atttggcgaa attattttta gcttcgaaaa ttacagcaat tgagttttca
     2941 gagcgaattt gtgttgaacg gagaagattg tatggtgtta aggatttgtc tccgaatata
     3001 ttaaattgtg gggaagagtt gtttatggct gctgagcgat ttgagcctga tgcagatagg
     3061 gctgattatg aaattgatga taatggactt aaggttgagg tccgatctat cttggaaaaa
     3121 tttaaattat aatcatgagt gattttaata tctggcattt aagtataaat gtcagatatt
     3181 cactcatgac aaggagatgt catgaaaaaa aagatagggg gatctatgat tctcgttctt
     3241 gctgttttgt gtctgactgc ttgccaagct aactatgtaa gggatgttca gggtggaaca
     3301 attgctccat catcatcttc gaaactaatc ggggtggcgg ttcagtgaaa aatattaaag
     3361 gatcttcttg agatcctttt tttctgcgcg taatctgctg cctgtaaacg aaaaaacccg
     3421 ccttgtggcg ggtccttccg tccgatcaga agtcttcgac ctcttctaat cggcggtggt
     3481 aactggcttg gaggagcaca gtcaccaaaa ctgtcttttc agtttagcct taactggttc
     3541 ataacttcaa gagcaactcc tctaaatcag ttaccaatgg ctgctgccag tggcgttaag
     3601 tcgtgtcttt tccgggttgg ccccaagaga acagttaccg gagaaggtgc agcagtcggg
     3661 ctgaacgggg ggttcctgca tacagcccag cccggagcga actgcctaag tcgccccgag
     3721 ataccagaag acgtgagcta tgagaaagcg ccacgtgtcc cgcaagggag aaaggcggaa
     3781 aaggtatccg gtaatcgata tggcgggaac gtgggggcgc aggagggagc gattcaccgg
     3841 aaacggtgat gatctttaag ccacgtcggg tttcgccacc gctggcggat ttcatggttg
     3901 agcctcacgg ctcccacaga tgcaccgaaa aagtgtctgt tttgtgaagt ccgtcagggg
     3961 ggcggagcct atggaaaaag cgcctccggc gcggcctttt tcggggtttt cgtttcttgc
     4021 tcacatgttc tttccggcgt tatcccctga ttctgtggat aacactatca ccgcttttga
     4081 gtgggcagat accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga
     4141 agcggaaaag cgcctggacg agcattttct ccttacgcat ctgtgcggca tttcacaccc
     4201 ggcatggtgc acttttcata ccatcctcac tgatgccaca tgattaagcc agtatacact
     4261 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgctaaa acccgctgac
     4321 gcgccctgac gggcttgtct gctcccggca tccgctcaca ggcaagctgt gaccgtctcc
     4381 gggagctgca tgtgtcagag gttttcaccg tcatccccga agcgtgcgag gcagctgcgg
     4441 taaagctcgt cagcgtggtc gtgaagcgct tcgcagatgt cagcctggtc gtcggcgtcc
     4501 agttcattca gtttttccag cagggtcagc gtctggcttc tgatgaagcg ggccatgtta
     4561 agggcggttt tttcctgttt agtcacagtt ctgtttctcc atgtgtgggg agtctgtcgg
     4621 aggtttttaa tgataccggt gaaacgcgaa aggatgctca cgatacgggt cacggatgag
     4681 gaacatgcgc ggctgctggc acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag
     4741 gtctgcctgg gggcaccgcc atcgaaaacg tcaggtcttc cgacactggc accgccgctg
     4801 ttgcgccagt tcgcttcggt cggcaataac ctgaatcaga tagcccggaa gataaacagt
     4861 ggacagtggt cggggcatga ccgggttcat gtggtggctg cactgatggc catcggacgt
     4921 gaactgagtg agctgaggga tgaggtccgt aaacagggag aacgcgatga tagttaagtt
     4981 tcatgccagg ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg gccgggagcg
     5041 taaccgggaa ggggcgagag ttctgcgggg tgctcccgaa gaggtgcggg aactcatcga
     5101 tgccacgccg tttgcgaaaa aatacacctc gggggttctg tcttttgcag agcagac
//
LOCUS       DAZKHK010000225         5134 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKHK010000225 DAZKHK010000000
VERSION     DAZKHK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.226"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..440)
                     /locus_tag="ACHOS5_004908"
     CDS             complement(<1..440)
                     /locus_tag="ACHOS5_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFP9779588.1"
                     /translation="MKTVNENFFNAIDDVSDTDTFDCVIMNPPFSLQYKAQSADIREK
                     ISAMMPYKKTSGVLDDAFYVLASMKARFSFFLCFPGVGYRRGELAMRQYFGNRVVELV
                     QISGGFADTSVSVLFVIIDNEKQSDDVVVYGVGFNGETCKKSAPV"
     gene            complement(775..851)
                     /locus_tag="ACHOS5_004909"
     tRNA            complement(775..851)
                     /locus_tag="ACHOS5_004909"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(815..817),aa:Arg,seq:tct)
     gene            complement(865..941)
                     /locus_tag="ACHOS5_004910"
     tRNA            complement(865..941)
                     /locus_tag="ACHOS5_004910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(905..907),aa:Arg,seq:tcg)
     gene            complement(1090..1842)
                     /locus_tag="ACHOS5_004911"
     CDS             complement(1090..1842)
                     /locus_tag="ACHOS5_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9779589.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEEQLRKVTR"
     gene            complement(1856..2845)
                     /locus_tag="ACHOS5_004912"
     CDS             complement(1856..2845)
                     /locus_tag="ACHOS5_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9779590.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2853..3668)
                     /locus_tag="ACHOS5_004913"
     CDS             complement(2853..3668)
                     /locus_tag="ACHOS5_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9779591.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     PTAINNIEKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3831..4226)
                     /locus_tag="ACHOS5_004914"
     CDS             complement(3831..4226)
                     /locus_tag="ACHOS5_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9779592.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACTAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            complement(4223..4549)
                     /locus_tag="ACHOS5_004915"
     CDS             complement(4223..4549)
                     /locus_tag="ACHOS5_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP9779593.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEVAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(4549..5043)
                     /locus_tag="ACHOS5_004916"
     CDS             complement(4549..5043)
                     /locus_tag="ACHOS5_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9779594.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTCRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLARQA
                     EELESRGLYRRAATVWMEAFRESHSQPERNNFLARRERCLRKSSKRTVSGEEWYLSGN
                     YVGA"
BASE COUNT         1257 a         1432 c         1191 g         1254 t
ORIGIN      
        1 accggcgctg actttttgca agtttcccca ttaaacccga cgccataaac aacaacatca
       61 tcgctttgct tttcgttatc aataatgacg aataatacag aaaccgatgt atcagcgaac
      121 ccgcctgaaa tctgaaccaa ctcaacaacg cggttgccaa aatactgacg cattgccagc
      181 tcaccacgcc tgtaaccaac cccgggaaag cacaaaaaaa atgaaaatct tgccttcata
      241 ctcgccagaa cataaaacgc gtcatccaga acaccggagg tctttttata aggcatcatc
      301 gctgaaatct tctccctgat atccgcgctc tgtgctttgt actgtagcga aaaaggcgga
      361 ttcatgatga cacaatcgaa cgtgtccgtg tcactgacat catctatggc gttgaagaaa
      421 ttttcattga ccgtcttcac tgtggcatcc ggaaagttat ccgggatatg caacaacgga
      481 gaggcctgta tatccacagc atacagactt cccaccgtga gaaactgctc cagttgcccg
      541 gagccacacg caccatcaaa aatactcagg ggcagatcac cacaatattc ccgcactttt
      601 tcagcgacaa accgacgcaa aggaacggac gtgatgtact ctgcgtattt tctggcgact
      661 tcgcgattat tggattcctg aaaaaccatt tgtgaaaggc tctgtggttg atatgcgccg
      721 ggcgtggcac ggagacaaaa aaggcccgca aaagcgagcc tgggaaaata agtgtggcgc
      781 gttgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct gtccggctga
      841 gctaacaacg ctgaataccg ataatggacc gccatcgggg actcgaaccc cgcgcagcca
      901 gctccgaagg ctggcgctct atcctgatga gctaatggcg gtatttaaca ggagatgcaa
      961 caaaaacccg ctcagcggcg ggctcttaaa tcttatcaac ggtagacata caaagcccat
     1021 cgttgggaaa actttatcca tattttttga aaaatgcaag catcatgtcg acatcttcgg
     1081 cgaaattatc tatcttgtca cttttctcaa ttgctcttca gcatacgctt cttcctgcca
     1141 gcactttgta accagtttat caatgacatc tgcatatcct ttgtaccact gataatccgt
     1201 caggtctggt atcagcttct ggacacgatg cctcgccagt gtggttggta aacggctaaa
     1261 ccggtttcca ttgcaacgcc cacaaatctt ataaacaggc gcgccatgaa gccgggttct
     1321 tttttcatcc aggacaatac ctttaccctt acaccctctg cacgctgtgc tgacttctcc
     1381 cttaccatgg caatgctgac atagttcctt cacccactct tccttgataa cagattcccc
     1441 gcttctggag tgtttcacca cttcgcgcaa tacattatga aatccagtac cagcacaatg
     1501 ctcacagcga gccttacttg ccgcagaccg ggaataatca gcaaaggcaa aattcacaag
     1561 gtaagggatg atctgtaacc gggtttcttc actcaattta ttcaatgtcg ggttatccag
     1621 tgccatcgcg taattgagta gaccttcaat cgcaaactga ggatcctgaa caccaacttt
     1681 tgccaggaat aaggcaaacc caagcggtgc tttcgactgc accatcccct gcgcagccat
     1741 cacatctgta atcgttaaac cacccgagcc tgtcgccggt gcgtcatcgc tcaattttgg
     1801 agattttggg gagtaatatt ttggtaaggc ttcaaggttc atgctcgttc tccacttacg
     1861 ccagtacgcc tattgccagc gcacgatcga taaaacgaaa tatcagctcc agctgggagc
     1921 catacttctc ttcgaatgcc acggtatccg catgcagctc gtcgtgatgc tttctgcaca
     1981 aaggcaacac aaagaggtca tgcgcttttg ttcccattcc accctgaccg tgacctatca
     2041 ggtggtgggg atcatcagcg ggctttccac aacatgcaca cggctgtgtc ttaacccagc
     2101 gcgtgtactt ttcattaacc cagcggcgac gttttgggcg taacataaaa gactccggcg
     2161 actccggatc cactttcagc gccagcacct tttttgcctt atcctggatg atgctggtgg
     2221 caggaaccga aggcacaagg tcactttccc gggtgacaga cggcacaaca agatttggta
     2281 atctcagtgc tttacgggct gcactttccg gtaaggcatc cgccaggtca ttacgaatca
     2341 gccaccagca cagttccggc attgtcacaa catgactatc atcaaaaccg agatcccgac
     2401 gcacaacaga caacacccag cgggcacagt tatccgtcgc cattgattcc agccgttccg
     2461 tgaactgatc gcgcagctgg ttatcgcagt gccagcacag acggattgcg cccggagcgt
     2521 gtcgcattgt ggtcatgttc tcgctgtgcc agtcggaatg aggccactgg cagccttttt
     2581 cacgaagtaa ccagctttca agacattcca cgccaccagc acgacggatc actgcctcat
     2641 tgcggaacac ggcccgaacg gcaggatcat ccgccagcgg ttgtgatgcc gccggaacgg
     2701 caccactggc gaaagatgaa taacgctccg gctcaggctc cagcaggaca cgcccctgca
     2761 taaacagggg catcagctct gaacctggcc tgaacaatac gatccccata cgcggggcga
     2821 tttcaggggt cagtagtgct ctcacggtca cctcaatgaa cggtatcgag cagctttaac
     2881 agctcaggga atcgggattc gaagaaatgc ggctgcgtct cgcgcggatt tgcaggactg
     2941 gtgatgttct tgccgaacat gcagcctttc gcggtcagcg accagaattt ttcgatgttg
     3001 ttaatcgcgg tagggctgta tcgttcgcgt tgttcaacga tccccagctt cgccatctgg
     3061 tgatatgcct gattagccgt caggcggata ccatactgct tcagcagtgc actcagcgac
     3121 agcgtagggc ggcttgaacc atctggcgca tcagcaggtg catcaatggc atagatcggc
     3181 ataagttcag gaagaccagc tacctttgat aatttctggt atgcaccaag tttcgaggag
     3241 tttgacagat ttagagtctt tgctgctgat tcaagcagaa tgaccccgga tttaattttg
     3301 tcggatgtgg tttcttctgg tgatgaatta tgaagcgcat caaaagtacg tatcactttt
     3361 aagctgaatg ccgggctgat ccacattgca tatgcataga ccagctcttt acagacatac
     3421 gtcccaccat tgcgcccctg aatggtgatg acaggaatac tacgggaatc tcccgtagtt
     3481 tcttcttcca ataattccac aagagccttc gtttcaggac gacgcataaa ctcgtgaact
     3541 tccagcgaac gggaggagcg attctcacca gcggcaagaa gagcagcttt ctgaaggtcg
     3601 ttaagacagt agttagattc gaagtactgg cgcacagaaa cgccatcaat tacaagcaac
     3661 tgattcattg gtttctccac aaatttttat ccacgagcgg gactgcactc ccttttcgtt
     3721 gatgcaggat gaacttactg cgatttttaa tagttatcaa ggatacactg ttcataaata
     3781 cagtatcttt aacgaggtaa tacccaaatt tagggtgttg ctcaattccg ttaccgagtt
     3841 gctaatttgc aactcgcttt ttcgtactta ctgatagtga tctcgacctt cccctccggg
     3901 ataaccggtc cccactccac cagcattctt ttcacctgac tgtcgtcttc ccacacaccc
     3961 gcgtgggtca gggcgtcaaa cagcgccttg ttatagttgt ccagatcgcg gatccggtta
     4021 tccggaggaa acaacacgat ctccactgaa gcaggtgccg acgttggttt cggcagacga
     4081 cgtaactgct caactattgc tgtgcacgcc gcactctgga attttcgccc cgccgcgctt
     4141 atcaggctct taccagcaaa tgcccctttg ttggggtgtc gccagtaggt gttcacgctg
     4201 ggtggaaaag gcagaatcag cttcatactt tcagccccct ctcatgtaac cagtgggctg
     4261 cacgcagcct ggcgttttcc tcaccagcaa gcagtgcgcg gataatcccg gccacctcgc
     4321 tgtcgtcgtc cttcaccgcg gtatgaagcg tgatgccccg ggccacgcca cgctttatcg
     4381 tgatgacgcc ttttttctcc agtgcgcgaa gatgctccac cgctgcattc actgaacggt
     4441 atcccagcat ggtagccacc tcctgattgg ttggcgggaa gccacgttct ttctggtaag
     4501 aaatcagcat atccagcacc tgctgctggc attgagttaa cgtcgtcatt aagcccccac
     4561 gtaattccct gacagatacc actcttcacc cgatacagtg cgcttgctgc ttttccgtaa
     4621 gcaccgctca cgacgcgcca gaaaattgtt tcgttctggc tgggagtggc tttcacggaa
     4681 tgcctccatc cacaccgttg cagcacgacg gtataagccc cttgactcca gttcttccgc
     4741 ctggcgggcc aggcacaaaa tcacccgggg atcgttagtg ccgacataga aattgcgcac
     4801 aggtctggat tcacgaactg gttgcggttc cggctcctgc ggtatctcag tcagccgcgg
     4861 gaaatgtctg catgtatccc cttcacaacg gtgagccaca cgcccactct gacgtaactt
     4921 gcttgctgac tgcagaacgc gctgtcgtga gtaacctgca aaagcatccg caatgtctcc
     4981 ggaagtacac cccggatggg cttcaatgaa tttctgaacg tcattcaaaa gactcatgat
     5041 caccccctga atcctgccgg gatctggctg tagtccacgt tgtcgtaact ggctttgaag
     5101 tacgggtcct cgcgtctggc tgcagatacc gcag
//
LOCUS       DAZKHK010000226         5078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKHK010000226 DAZKHK010000000
VERSION     DAZKHK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.227"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1703..2434
                     /locus_tag="ACHOS5_004917"
     CDS             1703..2434
                     /locus_tag="ACHOS5_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP9779595.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFESLKAKIQAYQRKKRRANKRVRATTLHYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIKLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSETRKASDKKPGESAVLWVKRGDVEAMQKVRNRALYLVKYE
                     TKQHDGSGQRNYGCSRGAGRLLDGR"
     gene            2792..3367
                     /locus_tag="ACHOS5_004918"
     CDS             2792..3367
                     /locus_tag="ACHOS5_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779596.1"
                     /translation="MNAIPYFDYSMAPFWPSYQNKIVGVLNRALREQSCSRVRRILLR
                     LPCEYDTTFSSRKTWLGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDSRSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGLF"
     gene            3436..3672
                     /locus_tag="ACHOS5_004919"
     CDS             3436..3672
                     /locus_tag="ACHOS5_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9779597.1"
                     /translation="MLTSITGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            3950..4450
                     /locus_tag="ACHOS5_004920"
     CDS             3950..4450
                     /locus_tag="ACHOS5_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779598.1"
                     /translation="MQITEALLSAPGEIRRFVQQAVDHWPNLLAFHFTLHSAEGNING
                     QQINAFRTSFYERITERNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSICHPRAR
                     LSVDEECSQVVDLLQQTWWMISAGGQCRVERCFRVTRPDTSEQYVALKTAVQSLMPLV
                     IATIIR"
     gene            4649..4913
                     /locus_tag="ACHOS5_004921"
                     /pseudo
     CDS             4649..4913
                     /locus_tag="ACHOS5_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         1271 a         1139 c         1206 g         1462 t
ORIGIN      
        1 ttatcattca ataaatgctc agttgtttta tttttccagt tatatccatt cacgttataa
       61 ccgccttcat attgtttcat ctgattacgc ctcaatcgtt ttcttttctg gtgattttca
      121 ccatcacatg tcagttgtat atgcaaggga atcaataaaa atatcaccct gcaagcactt
      181 caaagttgat tatggtgata ttctagaact attcacaaaa ctattccaga catatcaaga
      241 actatttctt gacctattca taataaaata aaacatgcta tcaactacag ctcatatcct
      301 ccctggaggt gtcgttgtac tgttcgactg ttcttgtgat caggttaaca ctctgaagag
      361 taaaaacgac cgtaatctgc ctgctatagg ggcgataaaa cactgaatgt gcatcctgta
      421 tgtgaaatcc ggtcatgtgg agcagtaagc aatgacagac atgcatgtta tatgtgaagg
      481 aggccgagag acgacataat cagtatggct gttattctgg ttgtaaaacg cgatcatttt
      541 ccggggaaaa gacgacatgg aagctgacga acgggatgtt ctggatggtc ttttcaggga
      601 ggctcggaat acggagtcag aaaaataatt ccagagtaaa tctgctccgt tttcagaaca
      661 aaaatcccca tgccggtcgt gaaaaactga aaccaacatt cagtctgcat gagtgtttga
      721 gaatgttgct aatgtcggta tgatactata tacacgtatt ttactaagta ttttctacta
      781 agtgttttat taaaaccttt tatacgcctt tctcattaac tcattttaac gaaattcatt
      841 aagcattgtc atacacattg ctttgtctga tacacatcca gtttagtaaa tctttacgtt
      901 agctaaaatc agtttaaagc tgctacaaaa caatctttct tgtttaagca atcaagatga
      961 cattctattt ttctgaagtt acgtttaacc ttttctgagt ttttgtgttt ttagtcgtta
     1021 acctgagaca agtgtcttta tgtaaaacgc aaatccggct gtgatttact gtgtgcttac
     1081 gatgattttg ttaaatggtt ttatggatga ttttgtaacg atgagattga tgagatgatt
     1141 aactggccct ggtgtttttt ctcagtgcaa ggctcttttc agaaaaagcg tgatgattgg
     1201 ttctggtgtt ttgattcaga tgaggtttac gttatcaata gttaatcgtt tttatgctgt
     1261 gtagtgaaac gtaacatttt caggatgaga aaatcgattc tgatattggt ggtttttgtt
     1321 cttatggcgg ggttatctgt aaacaggttt taagcgcact tattcggctc tgtgattgag
     1381 ttgtgtggtt aagccgtagg ttttggtgtt gttgttgatt tacagcgcca gaaagcgttc
     1441 tgattgcgtt tttggtgttt tgtttgcacg ttctgttgat ccgtttccct tctgactttt
     1501 ctcgtcgtcc atgtcgtttt taaaaaccat ttatgccgtt gttgtgtgtt ttatcctgcg
     1561 tgtaggttta tcttttcttc gtgggggatt cattctgttt taatcaggag ccattggcgt
     1621 tgctcaatgg cgggatgccg taaagtttta ccgcagtgat taatgatgtg aagtcaatcc
     1681 aaatcaacgg agatctctca tcatgaatca accaatacac aatgcttact ggttatcccg
     1741 ttttgaaagc attctcaaca gtgccctggc gcaacaccgt gccgtctcgt taatccgggt
     1801 ggatttacgt ttccctgagt atatgcctgc caccatcatg gataccgatt tagattcagc
     1861 ggtgatttct cgttttttcg aatccctgaa agcaaaaatt caggcttacc agcggaaaaa
     1921 acgacgagcc aacaagcgtg tgcgtgcaac cactctgcat tatttctggt gccgggagtt
     1981 tggcaaggaa aaaggcagga aacattatca cgtgatatta ctgctcaaca aagatacctg
     2041 gtgctcgcca ggggatttca ccgttccttc ttcgctggcg acactgatca agctggcatg
     2101 gtgtagtgct ctgcatcttg agccctggca gggtaatgga ctggttcatt tttccaggcg
     2161 gacgcctttc cgtaaaccga catcatctga tgctcgccct tcttccgatg atacgccttt
     2221 gtcgggtgga tgttctgaaa ccaggaaggc ttcagacaaa aagccgggtg aatccgctgt
     2281 gctctgggtc aagcgtggtg atgtggaagc gatgcagaaa gtccggaaca gagcccttta
     2341 tctcgtgaag tatgagacga agcagcatga cggttctggt caacgtaatt atggttgcag
     2401 tcgtggggca gggcgtcttc tggatggcag gtgagccctg taaaacggca tccggtacca
     2461 gagtgtatgt cacggtaagg gcgtcagcca acgcccttag atcgttttct gctaatcgcc
     2521 ctgaagtcat gaaacggtat ttttatctgc ttccgctcct ggaaacactc cgtcggtaaa
     2581 aacttaccgc cttgattaat gatgtgaacc gaagtcaacg gagatcattc atcctgaacc
     2641 tgtatccggt gttcgcccct tgtcggtgac ctgatcttcc cgttctgttt cggttcttca
     2701 cgtattccat caaccccatc cgcaaaccat aacacgtcag ctcattcacg ggcaggacgc
     2761 gttgtgggct gcgcataacg gagcatatct tatgaatgct atcccttatt tcgactatag
     2821 catggcaccc ttctggccat cttatcagaa caaaatcgtc ggcgttctca atcgtgcgct
     2881 gcgtgaacag tcctgctccc gggtacggcg gatcctgctt cgtctgccat gtgaatatga
     2941 cactaccttc agcagcagaa agacctggtt aggtatggat tttatcgaaa ccgtcagtgc
     3001 gctgatgaat gcgaaacccg gacgcgacct ttgctggctc ctgacccgtc atcctgaaaa
     3061 gccggaatac cacgtggtgt tatgcgtcag acaggagtat ttcgatggcc ctgaactgga
     3121 ccggctgatt ctggatgcct ggagcaacgt actgggcttc gcgtcaccgg gtgaagcggc
     3181 accgtaccag aaacagataa cccgggatgt gctgctggac agtcgttcac cggactgcga
     3241 agacatcctc aaagacttga tctgggcgtt cagtgatttc gcccgcgatc gtcgtggtgt
     3301 gtgcgatccg gaagcccgtt gcctcgccgg taatcccggc tatcccggat ctgcaggact
     3361 gttctgaaag ctcagttcag cagattcacc cgcatttgcc caacctccat aaatctcaca
     3421 gaggacatcc tgactatgtt gacctcaata acaggccacg acagtgtatt gctgcgtgcc
     3481 gacgatcccc tgatcgacat gaactacatc accagtttca ccggtatgac cgataaatgg
     3541 ttttacaagc tgatcagtga aggtcatttc cctaaaccca tcaaactggg gcgcagcagc
     3601 cgctggtaca aaagtgaagt ggagcagtgg atgcagcagc gaattgagga atcacgagga
     3661 gcagcagcat gaaacgtgtt gtaatgccag tacgctggca atgtgcgaaa tgccggcgct
     3721 ggtattgtgg aaatcagccc tgtccctggt gctggcgctg acaactcccg ctcatctttc
     3781 tgctggcacc ctctggccag ccaactgttt gtcatcattt cctgactgat tcgtcattcc
     3841 cttcgtcccg ccagtcactg gtgtttgtat cgtaccgtgc cggtgtcgat gtgcgctttc
     3901 cgtgtgtctg gtctgcaccg gcttaacaaa attcaacggg gataaaatag tgcaaataac
     3961 cgaagcctta ctttcagcgc cgggggaaat ccggcgtttt gttcagcagg cggtggacca
     4021 ctggccaaat ctgttggcat ttcacttcac gctccattcg gcagaaggaa acatcaacgg
     4081 gcaacagatt aatgcattcc gtacttcctt ttatgaacgt attactgagc gtaatcacac
     4141 tgccagtcca tcatcgccgg tggtattacg ctggctgcgg gaacaacatg gaggagcaac
     4201 aatacgatgc ctgttgctgc tcagccagac gagcatttgc catccgcggg ccagactcag
     4261 cgttgatgaa gaatgttcgc aagtggtgga tttactgcaa cagacctggt ggatgataag
     4321 tgctggcgga caatgccggg tggaaaggtg ttttcgggtt acccggcctg atacatcaga
     4381 gcaatacgtt gcgttaaaaa cggctgttca gtctctgatg ccactggtta ttgcaacaat
     4441 tatccggtga tgatcgggag aaatatcgac tcttccgtta atttttacag actgataaag
     4501 ttttgtctgc tgaatcttta tacagaattc tcttcagtgt gagtacacga attgatatga
     4561 gacgacaata actcagcccg cctggcgccg attattctgg ccggttttgt aacctgagtg
     4621 tcatttacat gatccaacag gaaatggcgt gacgctcaat acttctcagg tcagttacta
     4681 tatgactcag cgtaagaaag gtataactca gcatatctcg accatgaagg ctggtatctc
     4741 agtccgttct ggtcgtcgga tcgaaaaagg agattgggca aaaaaacagt gttcggcact
     4801 ggcgcacacg caaagatcct ctggaagctg tgtgggacag catgcttgtt cctctgttga
     4861 aagagaggcc ggctctgaca ccaacaactc tgctggagat gttacaggat aggtgtttat
     4921 ccaaacatca cagctaatga ggcagaagag gccatccatc agaaaaattg cggcgttggc
     4981 agtattgaca ctgcgggata tcgcatcagt aatctgtatc tgagagtggt ataaaaagac
     5041 atctgattct tcaatatttc acacaacgcc gtaagtaa
//
LOCUS       DAZKHK010000227         5053 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKHK010000227 DAZKHK010000000
VERSION     DAZKHK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.228"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..210)
                     /locus_tag="ACHOS5_004922"
     CDS             complement(<1..210)
                     /locus_tag="ACHOS5_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP9779599.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMG"
     gene            332..610
                     /locus_tag="ACHOS5_004923"
     CDS             332..610
                     /locus_tag="ACHOS5_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779600.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(602..892)
                     /locus_tag="ACHOS5_004924"
     CDS             complement(602..892)
                     /locus_tag="ACHOS5_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779601.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            1133..2779
                     /gene="cka"
                     /locus_tag="ACHOS5_004925"
     CDS             1133..2779
                     /gene="cka"
                     /locus_tag="ACHOS5_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFP9779602.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKL
                     NSYKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELA
                     KAESDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAV
                     ENELKNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEK
                     YAKIAQDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVTYDEW
                     AKHLEKISKALKVTGYLSFGNDVWDGTRKGLETGDWRPLFVTLEKSAVDFGVAKIVAL
                     MFSFIAGAPLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
     gene            complement(2776..3066)
                     /locus_tag="ACHOS5_004926"
     CDS             complement(2776..3066)
                     /locus_tag="ACHOS5_004926"
                     /inference="COORDINATES: protein motif:HMM:NF037571.4"
                     /note="Members of this family include Cki (colicin K
                     immunity protein) for the pore-forming toxin Cka (colicin
                     K activity protein), as well as immunity proteins from
                     other related colicin systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cki family colicin immunity protein"
                     /protein_id="HFP9779603.1"
                     /translation="MYLKYYLHNLPESLLPWLLYLTLNYNNDISLAFIFFASIHVLLY
                     PYSKLTIFSFIQKTTNIKKEPWYSYNLFYLLYLAMAIPVGLPSFIYYTLKRY"
     gene            3091..3222
                     /locus_tag="ACHOS5_004927"
     CDS             3091..3222
                     /locus_tag="ACHOS5_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP9779604.1"
                     /translation="MKIVLFLITILSLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(3433..3577)
                     /locus_tag="ACHOS5_004928"
                     /pseudo
     CDS             complement(3433..3577)
                     /locus_tag="ACHOS5_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314450.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            3951..4139
                     /locus_tag="ACHOS5_004929"
     CDS             3951..4139
                     /locus_tag="ACHOS5_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779605.1"
                     /translation="MDNGVTAFEKGESARRSRTTERSESVSEEAEEHRKRHFLLTHLC
                     GISHPAWCTFHTMRPDAA"
     gene            complement(4291..4482)
                     /locus_tag="ACHOS5_004930"
     CDS             complement(4291..4482)
                     /locus_tag="ACHOS5_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP9779606.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADVCEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4550..4873
                     /locus_tag="ACHOS5_004931"
     CDS             4550..4873
                     /locus_tag="ACHOS5_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9779607.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            4863..>5053
                     /locus_tag="ACHOS5_004932"
     CDS             4863..>5053
                     /locus_tag="ACHOS5_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFP9779608.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELI
                     DATPFAKKYTSGVLSFAEQT"
BASE COUNT         1367 a          978 c         1356 g         1352 t
ORIGIN      
        1 ccccatatcc cggaactgct gaaagaacgg gagcatcata cgtatggtgt ctgctttctg
       61 ggtgtagaag ttaggatcaa atccatcatc tgaagcataa acataatgcc gggtatgtcg
      121 ttccaggctg gtctttggtg tgcatcctgt aagagctatt gttgctgtta tcagaggtat
      181 cagaagcagc ctggcagcgg cagatctcat aaacatctcc ttgcgttttt aatcttgata
      241 ctcagggagt tatatctccg aagagtatct cttatcatac gtcggtttac tcccaatgtg
      301 atttgatggt caatgagtca ggaataatcc cgtgaaaaaa tgtgtgccag tcctgctttt
      361 tagtgttctt tctgcttcgg ttgccagtca tgcggacagc aatgtttttc aacaaaaggc
      421 cgagaagaca aaaaacggca ttgttgcgga tgttaatgcc agacagaaaa tgttcagtaa
      481 cagactgaag atacaacttc agatcgatga gttgagagca gagctggctg gcaccagaga
      541 tgcccaaaaa agagaggatt tgcgtaaaaa actggagtct ctggagcggc aaaagaaatc
      601 cttacgctga tgggggcgta ttttccccgg tttcttccat aaccttttta ccgttcagat
      661 atttacgggc gaggcgaatg cgtcttgcct ggtataccgc tgagtaagcc tgggtgggat
      721 tcacaccatt actgatttca ctgaccacat gacgctgatc agccagtgtc agcgtgtaaa
      781 cctgtttctg gatgaggtca cgggctttgt catactcata tccggcagca acaagggtgt
      841 cgagaagtcg tttatctgtt ttgttcagtt tatggcgggg tgatttgctc acatcgttct
      901 ccgttttttc atgatccaac agttttatat ctcatcctga tttgctccgg tgttctgttt
      961 cgacgttctc aatgcctgcc tccccatagt gtgatcctta ttgcaaaaat gaaaatccat
     1021 gctcttgaca cggacaatgc tgagtagtag gttttcactg tacataaaac cagtggttat
     1081 atgtacagta tattgttttt aatttattgt tttaattatc aaagaggaat ttatggctaa
     1141 agaactaagt ggatatggcc caactgctgg tgagtctatg gggggaacag gggcgaatct
     1201 gaatcagcaa ggtggaaata ataatagtaa tagtggggtt cactggggtg gtggctctgg
     1261 gcatggtaat aatggtggtc agggaaattc aaattcatct ggttctacat caactgtgat
     1321 gaaaaccggt gagtcatatc tgactccgtg gggtgatgtt gtcattaata atgatggcct
     1381 tccggtgatg aatggtattg ttatgacaga agagaattca actctggttg ataatccatt
     1441 tggaggggtc tccagagttc ttaattcttt aattagtgat atgccatcac tttttgcaga
     1501 aagctcagga aataacaaca ataacactgc ttcagttaat actgcaccaa cgaatgctca
     1561 ggtcagtgat atggataaga gttcgaaggt ggtgggcaat gtaattaatg aaaaacaaaa
     1621 gcagaagaat aaaattgcta cacagattag tgaaaaacag aagaaaatcg aggagatgaa
     1681 aaaagtattc aagcatcatt cttatcatgg tatcactgat cttgagaggg atgttgatga
     1741 gttgcaaaaa aaatcaaatc agcttgatgc agatattagc aaacttaatt cttataaaaa
     1801 tacattgcaa agtaaaattg gtgatgtgaa taaacagaag gaagctgaag aaaaggctcg
     1861 tgaaaatgca gaggtcgctg aacatgaaac tcttaatgaa gagaaacagg cggtggcgga
     1921 agcagagaag cgtttagctg aggcgaaagc tgaactggcg aaggctgaaa gtgatgtaca
     1981 gagtaagcaa gctactgttt ccagagttgc aggagagctt gaaaacgctc agaagagtgt
     2041 tgacgtgaag gtcaccggat ttccgggatg gcgtgacgtt cagaaaaaac tacagcgtca
     2101 gcttgaagct aagcaggctg agtactccgc agttgaaaat gaacttaaga atgctgttag
     2161 tttcagggat ggtaaggccg ctgaggttaa agaggcagag cagaaattaa aagaagctca
     2221 ggatgctctt gaaaagagcc agataaaaga tgctgttgat actatggttg gattttatca
     2281 atatataacc gaacaatatg gagaaaaata cgcaaaaatt gctcaggatt tagcggaaaa
     2341 gtctaaagga aagaaaattc aaggtgttga tgaagctctg gcagcatttg aaaagtataa
     2401 gaatgtactg gataagaaat tcagtaaagt tgatcgggat gctattttta atgcgttaga
     2461 gtctgttact tatgatgagt gggcaaagca tctagaaaag atctctaagg ctcttaaggt
     2521 taccggatat ttgtctttcg ggaatgatgt atgggatggt acccggaagg gattagaaac
     2581 aggagactgg aggcctttat ttgtcactct ggagaagagt gcggtagatt tcggagtggc
     2641 aaaaattgtg gcattaatgt tcagttttat tgctggtgct cctcttggtt tctgggggat
     2701 tgctattatc actggtattg tttcttctta cataggggat gatgagctga acaaacttaa
     2761 tgagttgtta gggatttaat atctctttag agtgtaatat atgaaacttg gcaatcctac
     2821 tgggattgcc attgccaggt aaagtaaata aaaaaggttg tatgagtacc aaggctcttt
     2881 ttttatgttt gtcgtctttt gtataaaaga aaatatcgtt agttttgaat atgggtatag
     2941 taatacatgt attgatgcaa aaaatataaa agctagagat atatcattgt tatagttaag
     3001 tgttaaatat aataaccatg gcagaagtga ttcaggtaaa ttatgtaggt agtattttag
     3061 atacataatt ttttcttttg ttggaggtgt atgaaaatag tgctttttct aattacaata
     3121 ttatccttgt caggatgtca agcaaactat attcgtgatg tacaaggagg aaccgttgcg
     3181 ccatcttcgt cttctgaact gacggggatc gcggttcagt agaaaagatc aaaggatctc
     3241 ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac ccgccttgtg
     3301 gcgggttctt ccgtccgatc agaagtcttc gacctcttct aatcggcggt ggtaactggc
     3361 tatcgcagag cacaggtacc taaattgtcc ttctagtgta gtcaaaatca gactgatact
     3421 tcaagaactc tgttaccaac ttataaatct cgctctgcta acccggttac caatggctgc
     3481 tgccagtggc gttaagtcgt gtcttttccg ggttggaccc aagagaacag ttaccggaga
     3541 aggcgcagca gtcgggctga acggggggtt cctgcataca gcccggcccg gagcgaactg
     3601 cctaagtcgc cccgagatac cagaagacgt gagctatgag aaagcgccac gtgtcccgta
     3661 agggagaaag gcggaaaagg tatccggtaa tcgatatggc gggaacgtgg gggcgcagga
     3721 gggagcgatt caccggaaac ggtgatgatc tttaagccac gtcgggtttc gccaccgctg
     3781 gcggatttca tggttgagcc gtaaggctcc cacagatgca ccgaaaaagt gtctgttttg
     3841 tgaagtccgt caggggggcg gagcctatgg aaaaagcgcc tccggcgcgg ccttttccgg
     3901 ggtttttgtt ttttgcgcac atgttctttc ccgggttatc cccggattct gtggataacg
     3961 gggttaccgc ctttgagaaa ggagagtccg ctcgccgcag ccgaacgacc gagcgtagcg
     4021 agtcagtgag cgaggaagcg gaagagcacc ggaagcggca ttttctcctt acgcatctgt
     4081 gcggcatttc acacccggca tggtgcactt ttcatacaat gcgccctgat gccgcatgat
     4141 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     4201 gctaaaaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctcacaggcc
     4261 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaagcg
     4321 tgcgaggcag ctgcggtaaa gctcgtcggc gtggtcgtga agggcttcgc agacgtccgc
     4381 ctggtcgtct gcgtccagtt cattcagttt ttccagcagg gtcagcgtct ggcttctgat
     4441 gaagcgggcc atgttaaggg cggttttttc ctgtttagtc acagttctgt ttctccatgt
     4501 gtggggagtc tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga tgctcacgat
     4561 acgggtcacg gatgaggaac atgcgcgact gctggcacgt tgtgagggaa aacaacttgc
     4621 gtcatggatg cgtaaggtct gcctgggggc accgccatcg aaaacgtcag gtcttccgac
     4681 actggcaccg ccgctgttgc gccagttcgc ttcggtcggc aataacctga atcagatagc
     4741 ccggaagata aacagcggac agtggtcggg gcatgaccgg gttcatgtgg tggctgcact
     4801 gatggccatc ggacgtgaac tgagtgagct tagggatgag gtccgtaaac agggagaacg
     4861 ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg gttgattacc
     4921 tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct cccgaagagg
     4981 tacgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg gttctgtctt
     5041 ttgcagagca gac
//
LOCUS       DAZKHK010000228         5006 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKHK010000228 DAZKHK010000000
VERSION     DAZKHK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.229"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            298..1272
                     /gene="hemB"
                     /locus_tag="ACHOS5_004933"
     CDS             298..1272
                     /gene="hemB"
                     /locus_tag="ACHOS5_004933"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP9779609.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1312..2163)
                     /gene="tauD"
                     /locus_tag="ACHOS5_004934"
     CDS             complement(1312..2163)
                     /gene="tauD"
                     /locus_tag="ACHOS5_004934"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP9779610.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHHV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLRGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(2160..2987)
                     /gene="tauC"
                     /locus_tag="ACHOS5_004935"
     CDS             complement(2160..2987)
                     /gene="tauC"
                     /locus_tag="ACHOS5_004935"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP9779611.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILLTVWWVVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2984..3751)
                     /gene="tauB"
                     /locus_tag="ACHOS5_004936"
     CDS             complement(2984..3751)
                     /gene="tauB"
                     /locus_tag="ACHOS5_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP9779612.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQIQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3764..4726)
                     /gene="tauA"
                     /locus_tag="ACHOS5_004937"
     CDS             complement(3764..4726)
                     /gene="tauA"
                     /locus_tag="ACHOS5_004937"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9779613.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIMNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1209 a         1412 c         1235 g         1150 t
ORIGIN      
        1 atagaccatg aaaatataaa acaacaaaaa cagacaaata atgtatcaat aagcaatgaa
       61 ctttaagcta ttcacttttt gaaaacgtca ccaaatcccc aaaaacaata atctatggct
      121 ataaataaat attaaaaata ttttcagtga atatgaaatt attgctattt ctattacaaa
      181 gatgtcctga cctctttttc attctcttct tgtcaacatc gcgacaactt tcgtaaaaca
      241 ctcctgcccc gcttcaggta tactgtgcac cttaattcca caaacatcag gcagaccatg
      301 acagacttaa tccaacgccc tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt
      361 gaagagacaa cacttagcct taacgacctg gtgttgccga tctttgttga agaagaaatt
      421 gacgactaca aagccgttga agccatgcca ggcgtgatgc gcattccaga gaaacatctg
      481 gcacgcgaaa ttgaacgcat cgccaacgcc ggtattcgtt ccgtgatgac tttcggcatc
      541 tctcaccata ccgatgaaac cggcagcgac gcctggcggg aagatggact ggtggcgcgt
      601 atgtcgcgca tctgcaagca gactgtgcca gaaatgatcg ttatgtcaga cacctgcttc
      661 tgtgaataca catctcacgg tcactgcggt gttctgtgcg agcatggcgt cgacaacgac
      721 gcgactctgg aaaatttagg caagcaagct gtggttgcag ctgctgcagg cgcagacttc
      781 atcgcccctt ctgccgcgat ggatggtcag gtacaggcga ttcgccaggc gctggacgct
      841 gcgggcttta aagatacggc gattatgtcg tattcgacca agttcgcctc ctccttttat
      901 ggcccgttcc gtgaagctgc cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg
      961 aacccaatga accgtcgtga ggcgattcgt gagtcgctgc tggatgaagc ccagggcgca
     1021 gactgcctga tggttaaacc tgccggagca tacctcgaca tcgtgcgtga gctgcgtgaa
     1081 cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg agtacgcgat gattaagttc
     1141 gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc tcgaaagctt aggttcgatt
     1201 aagcgtgcgg gtgcggatct gattttcagc tactttgcgc tggatttggc tgagaagaag
     1261 attctgcgtt aatgatcatt atgccgggtt atccaacccg gcatttcccc cttaccccgc
     1321 ccgataaaac ggaatatccc caagaatcgt cgcccgatgc ataatccgcc gctgtggcag
     1381 gtaatccgca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     1441 ttgccagcgc cagcgcacct gaaactccgg tttagtgata tgggcaaaca aaaaacctaa
     1501 caaggcttcg ctctctttct cgctgacatc aacaattcgt gtggtaaaac cttcgttcac
     1561 aaacagcgcc tgtttaccgg tcaccggatg cgttcgcacc accggatgta gcaacggtgg
     1621 atttttcgcg accgcctcac gccagcgttg atgctcagcc tcggttttgc ggtacttgta
     1681 ttccgggaac gatttacgga aatcatgctc cgcacgcaac cctctcaaca gctggcggaa
     1741 gggaacagag agcgcctcat atgcggcaat accactggcc cagagcgtat cgccgccggt
     1801 cgagggtaac tctttcgccg ccagaatcgc ccctgcgggt ggcgtttgaa taaatgtcac
     1861 atcggtgtgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca ggacgatgat
     1921 ctcgtcaacc ccttcagcat gagggtaaac agggtgaata tgcagctcgc caaaacgctg
     1981 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaaca ccacatgatg
     2041 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgttaagtc
     2101 ggcccccgta atttgtgcgc caatatacgg ccccagcggg gtaatgctca gacgttcact
     2161 cattgtactt ctccatgcca gggcgtcagg cggcgctgta acgcgcgcag acccagttct
     2221 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gcaccacgtc agtcgcgaga
     2281 aattcacccg ctgactgaac cataaatcct aaaccgcgcg ttgcggcaat cagctccgcc
     2341 gccaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     2401 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     2461 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccccg ccagcgccga catcgccacc
     2521 ggcgcaaaaa tcgctagata gatcagtaag atcttcgagg tttcgccaat accaaaccag
     2581 atcaccatca gcggcaaata agccagcggc ggcaccggac gataaagctc gattatcgga
     2641 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     2701 aacaccgccg cgagtagcgc cagcacaatg cgcgtcaggc tggctgccag atgctgccac
     2761 agcgtggcgt ccataaatcc ctgcggcccg gcaatggtga gcagttttgc cagtacctgt
     2821 tgcggtggcg gcaaaaacag cgggctaatc agttgcagcg ccgcgactac ccaccataca
     2881 gtgagtaaaa tcgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     2941 catttcagcc gatgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3001 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3061 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3121 ccagcacacg gcctgggccg ggtgaaagca gaaccagttc agtcgccata aacaccgctt
     3181 cttctatatc gtgggtaatc aacagcacct gctttcccgt ctcctgccag agtttcagca
     3241 gcagggtttg tatctggtcg cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta
     3301 ataacagctg gggatttgcc gccaacgcgc gggcaatccc cacccgctga cgttgaccac
     3361 cggaaagctg ccagatgtag cgtttttctg cgccttccag cccgactttt ttcaccatct
     3421 ggtgtgcgat ttccagtcgc tgcattttct ctacacctgc cagttgcagg ccgaacgcca
     3481 cgttgtcctg tacattgcgc cacggtaaca gcccttcatt ctgaaaaact acgccacgct
     3541 ctgctcccgg tccctgaata cccttacccg ccagttgaat gctgccatgc tgataaggca
     3601 caaaaccagc aatcaaattc agcaacgtag ttttgccgca gccagacggc cccagaacta
     3661 ccagaagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     3721 aatcggcgta aagatgagaa atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     3781 aggtaacgta ctggctgtaa tcattcgcta cagccgggac tttgccctgc tctttcaaaa
     3841 actgcgcggt gtcgatgatc gctttgttca ccggtccggt cagttctgcc gtttgttgct
     3901 gcggcgtcag ataggtattc cccttcacca gccccggaac gtcaccttcc ggcacgccgc
     3961 ttaaccgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtct gggttagcaa
     4021 tgtacggttg ctgagcatcg atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt
     4081 tctcggcaaa atctttccgc acgacccata cgtccagtgt cggcgcgccc cactgcccga
     4141 cctgttcaga atcggtcagc accttgccat ctttttccag ggcgttaacc gccggtgccc
     4201 agacataagc accatcaata tctccccgct gccatgcggc gataattgcg ggcggctgca
     4261 ggttcataat ctcaacttgc ccaggtttaa taccccagtg tttcagcgcc gccagcaggc
     4321 tgtagtgggt ggtggagata aacggtacgg cgatgcgttt accaatcaag tcttccggtt
     4381 tactgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     4441 agacttcaat cggcacctgt tggctggttg caaccgctaa cgggctggaa ccgaggttgc
     4501 cgatttgcac atcgcccgaa gccagcgccc gcacgatgct ggctccactg tcaaacttac
     4561 gccaatccac ggttgctccg ctttctttag caaaggtgtt gtcggcctga gccactttcg
     4621 ccggttcggc ggaggtttga tacgccacgg tgacgttcac cgcctgcgcc tgaaaagcga
     4681 tgaatgccag tgcggcaaga aatgtgttac gcgatgaaat tgccatgatt gtctgctccc
     4741 ccgtcttgtt atgggagcag tattcaggaa taaaaacatt cattaaaaga attagtcgtt
     4801 atcgtacaga tgattttatt cttagcaaaa aagcggcaaa ggaaagatta aatatgcaat
     4861 tacgcccact ctgataactc agtaaattac tgcacttcat tttattaatg cataaacaag
     4921 caggttctaa tgtctgttat tttattaaca gcaacacata taatgaaacc attatattca
     4981 cattgcatat ataaatgtat cattct
//
LOCUS       DAZKHK010000229         4994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKHK010000229 DAZKHK010000000
VERSION     DAZKHK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.230"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..862)
                     /locus_tag="ACHOS5_004938"
     CDS             complement(104..862)
                     /locus_tag="ACHOS5_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9779614.1"
                     /translation="MDSDFVSADRLMRALSNGEFEPYLQPVVSASDLTVSGAELLVRW
                     HMPTGEIIPPAYFINRVESAGLVLPLTEKILNRAVAGLSKVKAILPRGFRLAVNVTPS
                     LLAEREFTQMCLALAGHDSIHLVLELTEQQPFYMNRQTERVLSRLSDAGVAFALDDFG
                     TGSSVLSYLKHFPISYIKMDKSFTQDILSEKTSRYIVESVVGLAGKLGIDMVAEGVET
                     QAQVSCLCSLGVDYLQGFYFGRPEKVDTFCCEYR"
     gene            complement(852..1493)
                     /locus_tag="ACHOS5_004939"
     CDS             complement(852..1493)
                     /locus_tag="ACHOS5_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779615.1"
                     /translation="MSGTDTGDVLCGTDVVKVVSMRQKILLTGTCWYELTGLWHLLSA
                     QGHNVYRVPPGYPCARHGWDLIIVALSAEPVTGWGRHLSWIRELRAEMSGEMLVLVPE
                     RLEMLKVLRNICPVYSGCMPLSCLERTVRMALNRKTARTGKFRLTSGQRQALKRLSER
                     GRDRPLNLKQSERGLYWHYARLAENVGVRDFRMLLMTGLDREVHKMEDRQYGQ"
     gene            complement(1445..1567)
                     /locus_tag="ACHOS5_004940"
     CDS             complement(1445..1567)
                     /locus_tag="ACHOS5_004940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779616.1"
                     /translation="MARQRKVLIPMWWVVGVCVAATCMACLVLTLVMFCAVLMW"
     gene            complement(1567..2340)
                     /gene="faeJ"
                     /locus_tag="ACHOS5_004941"
     CDS             complement(1567..2340)
                     /gene="faeJ"
                     /locus_tag="ACHOS5_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria accessory protein FaeJ"
                     /protein_id="HFP9779617.1"
                     /translation="MLNIIHRLKSGMFPALFFLTSASVLAQPLTIPPGHWQEGMAVGV
                     TEFSGTLYAPEVSWQWQPRAVRMSAPDAVQAGLTTGKSGMVSESRRGQDFYILGGHTT
                     SLTTARPGLQPSVTLLQVAPSSPRIAARGELARGQVRYGEITFTMRHLLAWQDNITAG
                     QGWSVVSGEVTPEAEKQVKRQLWQVNGYEWTPDYAGLTARPDAFISGAESLLSQENGS
                     QHIAGAWVTSLSDVRVNFPGAEEPVKRWQGNLTPVVVYF"
     gene            complement(2357..3121)
                     /gene="faeI"
                     /locus_tag="ACHOS5_004942"
     CDS             complement(2357..3121)
                     /gene="faeI"
                     /locus_tag="ACHOS5_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeI"
                     /protein_id="HFP9779618.1"
                     /translation="MKKVTLFLFAASLLPSCVLAWNAPGEDFSGELKLEGPVTSTRNP
                     WVWKVGRGNEGLEVKQSRGVRDGEQAIPVALPAMTVLLGKTTLTTPAGREGLSPRVSY
                     GKGTEGFSLEWTAPGVAEVTLPVTGDNNVRAGTFVFRMQAAGVLRHMQDGQPVYAGVY
                     DDLKANGLPDESSTMKASDIPGVLQKMFSGEGPGWLQTMTISGYSGVSHFSDASLRQV
                     EGAYGAQTVAGSGELRLNGMMPERWRVSLPVSIEYQ"
     gene            complement(3152..3949)
                     /gene="faeH"
                     /locus_tag="ACHOS5_004943"
     CDS             complement(3152..3949)
                     /gene="faeH"
                     /locus_tag="ACHOS5_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeH"
                     /protein_id="HFP9779619.1"
                     /translation="MKIPHHYKSLLSAIISVALFYSAAPHADILDGGEIQFNGFVTDE
                     APKWTWQISSPDQTWSVDTADARTENGQLIFNLRDKGALPFLEGHLHEVAERGGPGFT
                     PFITFSSNGQPFTVTEGNGTSAQHFRASVPVRDPETGNVSGQLSFTLNQGMAVSAGRQ
                     EDGASVPVGMSLVSGQSVTDVQSGTLPQGLKARLSSLLLMNQNFGNGMNAVDNGQVIS
                     QGVLADGRVMNLAAAYASVVSDFELRLPAEGTPAAWQAGLNVTVTVQ"
     gene            complement(4132..4929)
                     /locus_tag="ACHOS5_004944"
     CDS             complement(4132..4929)
                     /locus_tag="ACHOS5_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779620.1"
                     /translation="MKKTLIALAVAASAAVSVSGSAMAWTQSGTGGNVNIGGTLTPAE
                     VTPWEVWIGTGVQDLNSLVAKGDTSVTIDVQKAVPVLGIRTVAKESFNGKVGISPQIS
                     YGSGVALDGFNAGVTTLTLDINDKTNQTKIGSVSVPFSAAAVASWDNPSSGNKGYRAL
                     YAANSGDGFFGGLGKSADKVAISHAALNLINDLNPDFSSNFNDFGLTIATSYAQTENF
                     QSAVVKHSAYYGSGIQAGAKATITLDNAVGDDAIEWEASLPVTVSYQ"
BASE COUNT         1276 a         1502 c         1070 g         1146 t
ORIGIN      
        1 aaaaatgcga tgtcggcaca tcatttatca gaaataaaat gaaattgctt aaatgtattt
       61 aattttattt ttcaggagtg acttactgat tacacagctg gcgttacctg tactcacaac
      121 aaaatgtatc cactttttcc ggtctgccaa aataaaaccc ctgcagataa tccactccca
      181 gagagcacag acagctcacc tgcgcctgtg tttccacacc ttctgccacc atatcaatac
      241 ccagcttccc tgccagcccg acaacgcttt ccacgatata ccgggatgtc ttttcactca
      301 gaatgtcctg tgtgaagctc ttgtccattt ttatataact gatcgggaag tgcttcaggt
      361 acgacagtac ggaacttccg gttccgaaat catccagtgc aaatgccact ccggcatcac
      421 tcagccggct cagcacccgt tcagtctgcc tgttcatgta aaacggctgc tgctccgtca
      481 gctccagaac cagatgtatg ctgtcatgcc ctgccagcgc cagacacatc tgagtgaact
      541 cacgctcagc cagcagggac ggcgtcacgt tcacggcaag ccggaaacca cgcggcagaa
      601 tggctttaac cttcgaaagc ccggccaccg cacgattcag gattttttcc gtcagaggca
      661 acaccagacc ggcagattcc acccggttaa tgaaatatgc cggcggaata atttctcccg
      721 tcggcatgtg ccagcgaacc agaagctccg caccggacac agtaaggtct gatgcagaaa
      781 ccaccggctg aagatacggt tcaaactccc cgttactgag cgcacgcatc agtctgtctg
      841 cggatacaaa atcactgtcc atactgtcgg tcttccattt tatgaacctc ccggtccagc
      901 ccggtcatca acagcatcct gaaatccctg acgcccacat tctctgccag ccgggcataa
      961 tgccagtaca gcccccgttc actctgtttc agattcaggg gacggtctct tcctctctca
     1021 gaaagacgct tcagtgcctg tcgttgtccg gacgtcaggc ggaatttacc ggtacgggct
     1081 gtctttctgt tcagtgccat gcggacagtt ctttccaggc acgacagcgg catgcatccg
     1141 ctgtacaccg ggcagatatt tcgcaacacc ttcagcattt ccagcctctc cggtaccaga
     1201 accagcattt caccggacat ttctgccctc agttcacgaa tccaggacag atgacgcccc
     1261 cagcccgtaa caggctctgc actcagcgcc acaataatca ggtcccagcc atgccgggca
     1321 cagggatacc ccggaggtac acggtaaacg ttgtggccct gtgcggataa caggtgccac
     1381 agccctgtca gctcatacca gcaggttccg gtcagaagga ttttttgcct catacttacg
     1441 accttcacca catcagtacc gcacagaaca tcaccagtgt cagtaccaga catgccatac
     1501 aggttgctgc cacacagaca ccaaccaccc accacatcgg tattaacact tttcgttgcc
     1561 tggccatcag aaatacacca ccaccggcgt cagattaccc tgccagcgtt ttaccggctc
     1621 ctcagcaccg gggaaattca cccgaacatc actcagggat gtcacccagg caccggcaat
     1681 atgctggctg ccattctcct gtgacaacag cgactcagcg cccgaaataa acgcatcagg
     1741 acgcgcggtt aacccggcat agtccggagt ccattcatag ccgttcacct gccataactg
     1801 gcgtttcacc tgcttttcgg cctccggcgt cacctctccg ctgaccacac tccagccctg
     1861 accggcagtg atattgtcct gccatgccag aagatggcgc atcgtgaacg tgatttcccc
     1921 gtaacgcacc tgcccccggg caagctctcc ccgggccgca atacggggag atgatggcgc
     1981 cacctgaagt aatgtcaccg acggctgcag ccccggacgg gcagttgtca gtgatgtggt
     2041 atgtccgcca agaatataaa aatcctgccc tctccggctt tcactgacca tgccgctttt
     2101 acccgttgtc agaccggcct gcacggcgtc tggtgcactc atccgcacag cacggggctg
     2161 ccactgccag gaaacttcag gagcatacag cgtgccgctg aattccgtta cccccacagc
     2221 cattccctcc tgccagtggc ccggcggaat agtcaggggt tgtgccagca cactggctga
     2281 agtcagaaaa aacagagccg gaaacatacc ggacttcaga cgatgaataa tattcagcac
     2341 cggaccgtcc tcctgcttac tggtactcaa tacttaccgg cagtgatacc cgccagcgtt
     2401 ccggcatcat gccgttcaga cgtaattcac cactgcctgc cacagtctgt gcaccatacg
     2461 caccttcaac ctgacgcagg gaggcatcac tgaaatgact cactcccgaa taaccactga
     2521 tggtcatggt ctgcagccag ccgggacctt caccactgaa catcttctgc agaacacccg
     2581 gaatatcaga agccttcatg gtcgagcttt catccggcag gccatttgcc ttcaggtcgt
     2641 catacacgcc ggcatatacc ggttgtccgt cctgcatatg acgcaatacc cccgcagcct
     2701 gcattctgaa aacaaatgtg cctgcccgga cgttattatc tccggtgaca ggcagcgtca
     2761 cctccgccac gcctggtgct gtccattcaa gcgaaaatcc ctcagtaccc tttccgtaac
     2821 tgacacgggg agaaagcccc tcacgtcctg ccggtgtggt cagggtggtt tttcccagta
     2881 aaacggtcat cgccggcagt gcgaccggaa ttgcctgctc accgtcacga acaccgcggc
     2941 tctgtttaac ctccagacct tcatttcccc gtccgacttt ccatacccac gggttacggg
     3001 tgctggtcac cgggccttcc agcttaagct ctccgctgaa gtcctctccc ggcgcattcc
     3061 aggccagtac acaggacggc aggaggctgg cagcaaacag aaacaacgtc acctttttca
     3121 cttctcttct ctccttatat ccctgcaacc atcactgtac cgtgaccgtc acattcagcc
     3181 ccgcctgcca tgctgcaggt gtaccctccg ccggcaaccg cagttcaaaa tccgacacta
     3241 cagaggcata tgccgcagcc agattcatca cccgaccgtc agccagtacc ccctgactga
     3301 taacctgtcc gttatccacc gcattcatgc cattgccaaa attctgattc atcagtaaca
     3361 gagaagagag tcgggctttc agcccctgtg gcagcgttcc tgactgtaca tccgtcacgc
     3421 tctgcccact gaccagcgac atcccgaccg gtacggatgc cccgtcttcc tgtctgccgg
     3481 cactgactgc catcccctga ttcagggtga aagaaagctg ccccgacaca tttcccgttt
     3541 ccgggtcacg caccgggaca gaggcgcgga aatgctgtgc tgaggtacca tttccttccg
     3601 tcacagtaaa aggctgtccg ttactgctga aggtaataaa gggggtgaat ccgggaccac
     3661 ctcgctcagc cacttcatgc agatgtccct ccagaaacgg cagcgcccct ttatcacgta
     3721 aattaaaaat aagctgtccg ttctctgtac gtgcatcggc agtatccact gaccaggtct
     3781 ggtcaggtga acttatctgc caggtccatt tcggtgcttc gtcagtgaca aaaccattaa
     3841 actgaatttc accaccatca agaatatctg catgaggcgc tgctgaataa aacagggcca
     3901 ccgaaataat ggcagaaaga agggatttat aatgatgcgg aattttcatc ggcattcact
     3961 ctgctttatc caaatatatg agtattatta atatcaagaa aacacgcagc acaaaaaatc
     4021 taataaacag tgtatccatg aagatataac aaagccgcgt aacatggcgc tgtaaaaaca
     4081 gcgccatgtt gtatttattc agatataaac aactttattg ttgcatctga attactgata
     4141 ggaaacagtg accggcaagg atgcttccca ttcaatcgcg tcatcaccca cagcattatc
     4201 taaagtaatg gttgccttag caccagcctg aatacccgag ccgtaataag cactatgttt
     4261 aacgactgca ctctggaaat tttcggtctg agcataagaa gtagcaatag ttaacccaaa
     4321 atcattaaaa ttacttgaaa aatcaggatt taaatcatta ataaggttaa gcgcagcatg
     4381 tgatattgct actttatctg cagatttacc caggccaccg aaaaagccgt cccctgaatt
     4441 tgctgcatat aaagctctat accctttatt accgctcgat ggattatccc atgatgctac
     4501 agcagcagca gaaaaaggca cggagacaga acctatcttg gtttgattag ttttatcatt
     4561 aatatccaga gttaaagttg taactcccgc attaaatcca tccagtgcaa ctccagagcc
     4621 ataagaaatc tgaggagaaa taccgacctt accattaaag ctttctttag caacagtacg
     4681 aattcccagc acaggaacag ctttctgaac atcaatggta actgaagtgt cacctttggc
     4741 gactagagag ttcaaatcct gaacaccggt tccaatccac acttcccatg gagttacctc
     4801 agccggggtt aaagtgcccc caatgtttac gttaccgcca gtaccacttt gagtccaggc
     4861 catcgcagag ccagatactg atactgcagc agaggctgct actgccagtg caatcagagt
     4921 ctttttcata aaataaatcc tttagtattt cataaataac acactaaatt ggtaattatt
     4981 attctagaat tacc
//
LOCUS       DAZKHK010000230         4964 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKHK010000230 DAZKHK010000000
VERSION     DAZKHK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.231"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(299..375)
                     /locus_tag="ACHOS5_004945"
     tRNA            complement(299..375)
                     /locus_tag="ACHOS5_004945"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(339..341),aa:Met,seq:cat)
     gene            584..1681
                     /gene="mltA"
                     /locus_tag="ACHOS5_004946"
     CDS             584..1681
                     /gene="mltA"
                     /locus_tag="ACHOS5_004946"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP9779621.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1758..2564
                     /gene="tcdA"
                     /locus_tag="ACHOS5_004947"
     CDS             1758..2564
                     /gene="tcdA"
                     /locus_tag="ACHOS5_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP9779622.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2615..3058)
                     /gene="csdE"
                     /locus_tag="ACHOS5_004948"
     CDS             complement(2615..3058)
                     /gene="csdE"
                     /locus_tag="ACHOS5_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP9779623.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTTVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(3058..4263)
                     /gene="csdA"
                     /locus_tag="ACHOS5_004949"
     CDS             complement(3058..4263)
                     /gene="csdA"
                     /locus_tag="ACHOS5_004949"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP9779624.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGIVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4455..4682
                     /gene="ygdI"
                     /locus_tag="ACHOS5_004950"
     CDS             4455..4682
                     /gene="ygdI"
                     /locus_tag="ACHOS5_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP9779625.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT         1211 a         1218 c         1316 g         1219 t
ORIGIN      
        1 ttaatttttc tactaattaa aaagttgtgt gctttttgta caatgattat ataagcgtag
       61 cgccctgttg tgatattgaa atgttaacat aaccaaaata aattgattta tagaatatat
      121 aaatgaaatt cattaatggg gtatatgttt aatatataaa taagatggat ggctttgatg
      181 gagggatatt aacggtttac tcattgtctc aggcttcact gaaacgggta aggaagcgta
      241 aaaacgaaaa aaccgcaagc caatagcagc tttgcggttt gtcagcattc taataaattg
      301 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
      361 ctgctccacc ccgcgtccgt ggatgcgcac tatactctgc tcgcgttttc atgcaacctt
      421 ttttttaccc cgatcacgga ttcggcatga ttttgaacaa aaacaaatca ctaaagccta
      481 ttttttgtgc aaaatttgcg tcagggagag gctgctcatt tcattaaacg cggtggtttg
      541 ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag gacgttgggt
      601 aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac
      661 cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc tggtgaacca
      721 gccagatgcc gttggcgcgc cgattaatgc cggtgatttt gccgagcaaa ttaaccatat
      781 ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg cggtgcaaga
      841 gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg atgcctggca
      901 gatggaaggt gtcgacaact atggtaacgt gcagtttaca ggctattaca cgccggtaat
      961 tcaggcgcgc catacccgtc agggcgagtt ccagtatccg atttaccgta tgccgccaaa
     1021 acgtggtcgt ctgccgtctc gtgcggagat ctacgcagga gctctgagtg ataaatatat
     1081 tctcgcttac agtaactccc tgatggataa cttcattatg gatgtgcagg gtagcgggta
     1141 tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga aaaacggtca
     1201 tgcctatcgc agcattggta aggtgttgat cgaccgtggc gaagtgaaaa aagaagatat
     1261 gtcgatgcag gcgattcgtc actggggcga aacacacagt gaagctgagg ttcgcgagct
     1321 gctggaacag aacccgtctt tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg
     1381 ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt caattattcc
     1441 gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca aatttaacgg
     1501 tcagtacgaa ttgcgtctga tggtggcgct ggatgtcggt ggcgcaatca aaggccaaca
     1561 cttcgatatc tatcaaggga tcgggccgga agccggacac cgcgcaggtt ggtacaacca
     1621 ctatggacgt gtctgggtgc tgaaaaccgc tccgggcgca ggtaacgtct ttagcggctg
     1681 atgtgatatt ctgtatgccg tttttacgga tatgacaggg tgaggggtaa cctcaccctt
     1741 tttaattctg aggtgctatg tctgtggtaa ttagtgatgc atggcgtcag cgttttggtg
     1801 gcacagcgcg tctgtatggt gaaaaagcat tgcaactgtt tgctgacgcg catatttgtg
     1861 tggttggtat cggtggtgtc ggttcctggg cggcggaagc gctagcgcgc acggggattg
     1921 gcgcaatcac gcttatcgat atggatgatg tgtgcgtcac caataccaat cggcaaattc
     1981 acgctctgcg cgataacgtc gggctggcaa aagcggaagt aatggcggag cgtattcgcc
     2041 agattaaccc ggagtgccgt gtaacggtgg tggatgactt cgtgacgccg gataacgtag
     2101 cgcaatatat gaatgccggt tattcgtacg tgattgatgc tattgatagt gtacggccca
     2161 aagcggcgct gattgcttat tgtcggcgca ataaaatccc gctggtcacg accggtggcg
     2221 ctggtgggca gattgacccg acgcagattc aggttaccga tctggcgaaa acgattcagg
     2281 acccgttggc ggcgaagttg cgcgagcgcc tgaaaagcga ttttggcgta gtgaaaaaca
     2341 gtaaaggtaa gctcggcgtg gattgcgtgt tctctaccga agcgctggtg tacccgcagt
     2401 cagacggcac cgtctgtgcg atgaaagcca cggcggaagg gccaaagcgg atggattgtg
     2461 cctcaggatt tggcgcggcg acgatggtga ccgccacttt tggctttgtt gcggtttctc
     2521 atgcgctgaa gaagatgatg gcgaaagcgg cgcgtcaggg gtaaacaatc aggtcggatg
     2581 cgacgcgaac gtcttatccg acctggttct gtgcttaaac ctgcttcgcc gcagcgataa
     2641 tcgcctcgct taacgcattc aacccctggc tgcgtgaggc gctaagttgc gcacgtaatc
     2701 ccagctcatc aaacaatgcc agcggcgact gtgcctgcaa ttcggcggcg gttttcccct
     2761 caacggtagt caacaacacc gccaacaggc cgcgcacaat gcgcccttcg ctgtcgccaa
     2821 agaaatgcat cttgccgttt tcagccacgg tatatcccag ccagacgcgg ttttcgcatc
     2881 cggcaatctc tttagcctgc gcttttaact cctctggcaa agccggaagc tgtttcccca
     2941 gcatgatcaa ctggcgatat ttatcttccc attgcgtcaa cggtgcgaag gtattgcgta
     3001 acgtttctgc ggttacggtt gtgccgaacg gatgtccggc gaattgcggg tttgtcatta
     3061 atccaccaat aattccagcg cgcggtcaac ggcattcacc agcgcatcca catcactctt
     3121 tgtattatat ggcgcaaatg aggcgcgcag tgtgccggtt acgcctaatt ctgccagtag
     3181 cggctgagcg caatgctgcc cggcgcgcag ggcaataccg tactccgcca gcagtgtcac
     3241 catatcgctg tgatgaacac ccgcaaaatc aaaggccagc aggctggaat cctggcagcg
     3301 gaatgaacga aagcctggac gtttcgccag cgcttcttct gcaagcgttg ctaagctacg
     3361 gctccagctt tcggcctggt tgatatcgta atctgtcagc cattccagcg ccgcgcttaa
     3421 tcctatgacg ccagcgacat ttggcgttcc tgcttccagt ttccacggcg cagattgagt
     3481 cgtgaagcct tcaaaactca cttcgtgaac cattttgccg ccgcccagcc agggcgacat
     3541 cgcttccagc agttctggtt tgccatacag cacgccgatg cctgtcgggc catacagttt
     3601 gtgacctgaa aaagcataga aatcaatatc cagttgctga acatccgcgg ggaaatgcac
     3661 cgccccctga gcaccatcaa ccatcaccac tatcccggct gaatgagcaa aagtaatcgc
     3721 tctcgccaga tccgggcaac cgccagtgac gttcgacatc tggcccaacg ccagaatccg
     3781 actacgggga gtaatcagtt ctggcaacaa atcgacatcc ggcagtcgct gcgcattaag
     3841 cggcaatttc accactttgg cccccgtttg ttgggcgacc atcagccagg ggacgaggtt
     3901 ggcgtggtgc tctgccacac tgacaataat ttcatcgccc ggttgcagac gcggacgggc
     3961 atagcattgc gccaccatgt tgatggattc agtggtgccg cgcgtccaga cgatattttt
     4021 gtcatccgat gcattcagta attgtgccac tttttcccgc gcagcttcgt aacgcgcggt
     4081 caggcgttgg gcttcggcaa actggctgcg atggacgttt ccggcgctca ggctataaaa
     4141 ctgtcgggta gcttcaacca cggcttcagg tttaagcgcg gtcgcggcgc tgtcgagata
     4201 gacgcccgca tcctgtagcg cgggaaactg ggcgcgaaac tgcgcgggat taaaaacgtt
     4261 catggtactc ctcggcttga tgcactgatc gtgacgcaaa attcactttg atacaaggag
     4321 agtgctaacc atcagaatta tctggatatc ctggcgaagc cgcgtcagta acatatgctg
     4381 aatagtgtta ggcgttaatt ttagcctgtt aatcaatact attaagattt attaatcgct
     4441 ttaaggagaa gatgatgaaa aagactgccg caattatttc tgcctgtatg ctgacttttg
     4501 ccctgagcgc ctgttccggt tcgaactatg tgatgcacac caatgacgga cgtaccatcg
     4561 tctctgacgg taaaccacag actgataacg ataccggtat gatttcgtat aaagacgcta
     4621 atggcaacaa acagcagatc aaccgtactg acgtgaaaga gatggtcgaa ctggatcagt
     4681 aattcgcgat cgcaaggtaa aaaaaagcac cgcaattagg cggtgctaca ttaatcacta
     4741 tggacagaca gggtaaatgt acaggaagtg aaaaaaggta gctttgctac catggtctga
     4801 atcgcagacc aattgcaaac acaacaacac aacatcacaa ccgtaagcca aaagttcacc
     4861 agaacacgca ttccgataaa acttttcgtt ccggctcagg aagtgccgcc actataggta
     4921 tttgctggta gaagctcaac ggacaattta taatggctca gatt
//
LOCUS       DAZKHK010000231         4930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKHK010000231 DAZKHK010000000
VERSION     DAZKHK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.232"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..587)
                     /locus_tag="ACHOS5_004951"
     CDS             complement(<1..587)
                     /locus_tag="ACHOS5_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779626.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSPGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGV"
     gene            complement(584..820)
                     /locus_tag="ACHOS5_004952"
     CDS             complement(584..820)
                     /locus_tag="ACHOS5_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779627.1"
                     /translation="MLNPLSLNIRRLLQRKKTSIPTVGQWYSTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLITFCSGKNMKHLGGAA"
     gene            complement(813..1523)
                     /locus_tag="ACHOS5_004953"
     CDS             complement(813..1523)
                     /locus_tag="ACHOS5_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFP9779628.1"
                     /translation="MRFSEIAAVYFHALNKMLVSGTHTQWKLIDLQSDLFTVVFDSIV
                     HELKHNKIVMFAQCIDSVIPGCVDNIYLSGHTLHIVAKSTIGSETPELSTAIYDAPAS
                     FLSSALTHARIMVGWAGEPKGSPVSVDAGSLNPVQSATSEIETSGGGSFPQSTEAAIM
                     ATVPAITRLNDEDLHKLSYITTALRALRKVTLSDPQAHQVLVETILNLQAERIRLADK
                     ANFHIHRLLNISGGHRHA"
     gene            1488..1718
                     /locus_tag="ACHOS5_004954"
     CDS             1488..1718
                     /locus_tag="ACHOS5_004954"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9779629.1"
                     /translation="MKVYGSNLGEPHTCAFGDGLFELRIKGSDGIARVFYCTLTGKRI
                     IMLHSFIKKTQKTPPAERKKAETRMKEVKHDW"
     gene            1708..2016
                     /locus_tag="ACHOS5_004955"
     CDS             1708..2016
                     /locus_tag="ACHOS5_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779630.1"
                     /translation="MTGKRTPPTMTHDEMAAKWMDDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGVSGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(2038..2211)
                     /locus_tag="ACHOS5_004956"
     CDS             complement(2038..2211)
                     /locus_tag="ACHOS5_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9779631.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEAPP"
     gene            complement(2357..2908)
                     /gene="ymfL"
                     /locus_tag="ACHOS5_004957"
     CDS             complement(2357..2908)
                     /gene="ymfL"
                     /locus_tag="ACHOS5_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP9779632.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQFLPLGWAMVLQRAAGTHHIADAVAQSAGGVFVSLPEIDEVENADINQR
                     LLEAIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(2952..3152)
                     /locus_tag="ACHOS5_004958"
     CDS             complement(2952..3152)
                     /locus_tag="ACHOS5_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9779633.1"
                     /translation="MKAYWDSLTKEQQGELAEKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            3243..3917
                     /locus_tag="ACHOS5_004959"
     CDS             3243..3917
                     /locus_tag="ACHOS5_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFP9779634.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYQIKA"
     gene            4277..4587
                     /locus_tag="ACHOS5_004960"
                     /pseudo
     CDS             4277..4587
                     /locus_tag="ACHOS5_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4602..>4930
                     /gene="yfdP"
                     /locus_tag="ACHOS5_004961"
     CDS             4602..>4930
                     /gene="yfdP"
                     /locus_tag="ACHOS5_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP9779635.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAG"
BASE COUNT         1321 a         1238 c         1149 g         1222 t
ORIGIN      
        1 tttactccct cattcaaacg ggctttccag gctttgaagg ctgcagattt tgaattgcca
       61 ccagcacgtt tggggtatgc cagccatgcc tgctcaaact ccggagagta ttccggtcgg
      121 tttgaacgaa ctcgcacaga ctcatcagca gatgcaccaa cagctattgg ttcattgact
      181 ggttctttga ctggttcaaa agagtgactg gttctgggtg aatctcctgc actaccccct
      241 ggtgcaactc ctgcactacc tggtgaattt gctgcaccag atagtgaatt atttgcacta
      301 ccccctggtg aatctcctgc accatccaga tgaaggagat agatattact tgagttacct
      361 ttttcacctt tccgggtaac tttttttacc agcccggact cacaaagggc cgcaatatga
      421 ttcatcacag aacgtttgct aatctcgcac tggtcagcaa tatgctggta gctgggccag
      481 cactccccct gatcgctggc attatcagcc agcttgatca gaaccagttt tcgcaatgga
      541 ttacccactc gaattttcat cgctttaacc atcagctcca tactcatgct gcacctccga
      601 gatgcttcat gttttttccg gagcaaaagg ttataagcgg catactgacg cggtaattac
      661 ggcccagcgg ttcacaaatc actttctgac attcacggtc aaccaggcta acacgtagaa
      721 catgccctgc gggcgtggag taccactgcc caactgtagg aattgacgtt tttttacgct
      781 gaagtaaacg gcgaatattg aggctcaacg gattaagcat gacgatgccc tccgctgata
      841 ttcaggagac ggtgaatatg aaaattagcc ttatccgcca gacgaatacg ttcagcctgc
      901 aagttaagaa tggtttctac cagaacctga tgcgcctgcg gatccgaaag agttaccttg
      961 cgcagagcac gtagtgcagt tgttatataa ctgagtttat gtaagtcttc atcattcaga
     1021 cgagtgatgg ctgggacagt agccatgatg gcagcctccg ttgactgtgg aaaacttcca
     1081 ccaccggagg tctcaatctc gctggtggcg gactggacag ggttgagact accggcgtca
     1141 acggagaccg gcgagccttt cggctcccct gcccagccca ccataattct ggcgtgcgtg
     1201 agcgcggacg ataaaaaaga cgctggcgcg tcgtatatcg ccgttgacaa ttccggggtc
     1261 tcactcccga tagtggattt tgccactata tgtaaagtgt gccccgacaa ataaatattg
     1321 tcaacgcatc ccggaattac agaatcaata cattgcgcaa acataacaat tttgttatgt
     1381 ttcaattcat gaactatact atcgaatact acagtgaaga ggtcagactg gaggtcgatc
     1441 agcttccatt gagtatgcgt gcccgatacc agcatcttgt tgagcgcatg aaagtatacg
     1501 gcagcaatct cggagaacct catacctgcg ccttcggtga cggacttttc gagcttcgga
     1561 ttaaaggcag tgatggcatc gcacgcgtct tttactgcac cctgacagga aaacgcatca
     1621 tcatgctgca tagcttcatc aagaagaccc agaaaacccc accagctgaa cgcaaaaaag
     1681 ctgaaaccag aatgaaggag gttaagcatg actggtaaac gtaccccgcc aaccatgaca
     1741 cacgatgaaa tggcagctaa atggatggat gacccggcct ttaaagcaga atacgacgcc
     1801 atcgccgacg agttcgcact gcttgatgaa atgctggccg cacgcaaaga agctggtctc
     1861 acccaggctg aagttgctga acgcatggga actaaagcaa ctgccatcac cagaatggaa
     1921 agcaatcttg cctctggtgt cagtggacca tcatttgcca cactgaaaaa atttgcacgc
     1981 gcaacaggga aaaaactcca gatccgcttc gtgtaaattt ccattattgc gccgttatta
     2041 tggcggcgct tcgcactcaa caaaatcacg cctgaatttc cacaaaggac taaagcactc
     2101 atgcgggtag tctttgcgaa gatagataac gcgctgtgtt tctggttccc aacgaataac
     2161 atgaacataa agtcctcttc cgtcacgaaa ccagcggtta agttcctgca caacttgccc
     2221 cccacagtca ggtaaagttc tctgtggtta cttacagcca ggtgatttgg taatctgcat
     2281 tcatgccgta acaacaggtg ttcagcgacg ctgaccacca gctgttgcga caaacggtta
     2341 tttgccgtta aactgttcat gcgttagttt ctccacagac acaaaacgcc acgacgcccg
     2401 gagctgcaca ctcgcgggcg tcactctttt ctggagcgca aaagattttg tagaccagtg
     2461 ctgcatgctc ctggagcttc gaaattgaca gatacaactc atcattaatt gctgtctgct
     2521 cgtgtggctc cactaccccg tcttcgattg ccgaacgaat ctgctttgag taactaccga
     2581 tctgttcgat ggcttccagc aatcgctggt ttatatcggc gttctctact tcatcaattt
     2641 caggaagcga tacaaacacc ccaccagcag actgtgcgac agcatccgca atgtggtgag
     2701 taccagccgc acgctgtaaa accattgccc atcccaacgg taaaaactga tcgccatcgg
     2761 cacgaaggcg attaaataat gcgttctctg ttacatccag ccagtcagcc gcttcagcgt
     2821 aaccccccgg caacgccgcg atagtttttc tgacagcttt cacgtaccac tctggctgtt
     2881 tttctacttt ccagtgatgc ttacccacgg ttagcctcat cgttctgtgg ttaaaaattg
     2941 aaggtgttct gttaatcttt cggatagata tccggtctta agtcagattt cgtaattgca
     3001 cctgacgtgc attgctcaag ttttttagcc agcacaaaac tggctttttt ataaccattg
     3061 aaaaccagcc gtaagtagcc tggtgttgag ccaacttttt cggccaactc gccctgctgt
     3121 tctttggtta aagagtccca atacgctttc atacaatatg tacctcccat atacatatta
     3181 catgattgag atgaaccttc aagatacttg taccttatcg gtacaaaggt tttaatttct
     3241 ttatgaaaac agtccatgac atccggcggt ctaacgccag aaaactgaga gatggtgttg
     3301 gcgggaattc ttcctttgcc accatgattg atcgcgagcc aacccagacc agcaggttta
     3361 tgggagatgg tgctactaaa aatatcggtg acagcatggc acggcacatc gaaaaatgtt
     3421 tcgacctgcc tgtcggatgg cttgatcaag aacaccagac cacgaacatc acaaaaaaac
     3481 ctgatgtttc aatcactaat aaacaaataa cgttagtccc tgtcatatca tgggtacagg
     3541 ccggagcatg gaaagaagtt ggctattctg aggttgattt gagcacagca gaaacttatc
     3601 cctgccctgt accctgtggc gaaatgactt atatcttgcg ggtgattggt gattcaatga
     3661 ttgatgagta ccgcccggga gacatgattt ttgttgatcc cgaagtccct gcctgccacg
     3721 gtgacgacgt tattgcattg atgcacgata caggcgaaac caccttcaag cgattgatag
     3781 aagatggaac acagcgttat ctcaaagcat taaacccaaa ctggcctgag ccttacatta
     3841 agattaacgg taattgctct ataattggta cagtgatttt ctcgggaaaa ccaagaagat
     3901 accaaataaa agcctaatca atatttataa cctgcttcgg caggtttttt tatacttgac
     3961 aatgtacccg atagatacat aatgtatcta aaagaaacat gccgcaggcg agattcaaac
     4021 aaagtttggt tgtaacacgg cgtatggcac atgcgtcgtt agcggtctgg ggacgttaaa
     4081 ggggacaatc cactccttgc tcgggcaaac aaaccaggta gccggaatgt gcaagtcaat
     4141 gatgatgctg ataagacgcc taaccagcgt ggcgattcgg tttgacgcct gggaagagac
     4201 cagggtgcaa cgatgaggga atttatggaa ccgcgacaaa gagtggtgcc gtaactggct
     4261 aagtgctctc agcgttgtgg tgaatgcggc tcagcgcacg cgggttaagg ttgagtctga
     4321 cagtcgacct tctgtggata cccacccgtc tggtgtgcaa ccttcgccag acaccgggag
     4381 gcacccggca ccacaacttt atgctgtgtg tagtcctggc ggtaccagtt tgtacacttg
     4441 cttccggctg gtaccgctct ttttacaaaa cagagaagag catcaccgga cgacgggctc
     4501 ataacccaat ccatccgggc ggctgccacc gcaggtgttc ttctctgttt tgtggagaaa
     4561 ccaaccgacc ttgcagggtc gatatgatga ggagcagcaa aatggctagc gaacgcagta
     4621 ctgatgtgca ggcatttatc ggggagctgg acggcggcgt atttgaaacc aaaatcggcg
     4681 cagttctcag tgaagtcgct tccggtgtga tgaacacgaa aaccaaaggt aaggtctcac
     4741 tcaacctgga aatcgaacca tttgatgaga accgtgtgaa aatcaaacac aaactctcat
     4801 atgttcgccc gactaaccgc gggaaaattt ccgaagaaga caccaccgaa acgccgatgt
     4861 atgtcaatcg cggtggtcgc ctgactattc tgcaggaaga ccagggacaa ttactgactc
     4921 ttgccggtga
//
LOCUS       DAZKHK010000232         4905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKHK010000232 DAZKHK010000000
VERSION     DAZKHK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.233"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..431)
                     /locus_tag="ACHOS5_004962"
     CDS             complement(<1..431)
                     /locus_tag="ACHOS5_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9779636.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKW"
     gene            551..1360
                     /gene="proC"
                     /locus_tag="ACHOS5_004963"
     CDS             551..1360
                     /gene="proC"
                     /locus_tag="ACHOS5_004963"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9779637.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1377..2492)
                     /gene="adrA"
                     /locus_tag="ACHOS5_004964"
                     /gene_synonym="dgcC"
     CDS             complement(1377..2492)
                     /gene="adrA"
                     /locus_tag="ACHOS5_004964"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP9779638.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2594..2914)
                     /gene="psiF"
                     /locus_tag="ACHOS5_004965"
     CDS             complement(2594..2914)
                     /gene="psiF"
                     /locus_tag="ACHOS5_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP9779639.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3033..4448)
                     /gene="phoA"
                     /locus_tag="ACHOS5_004966"
     CDS             complement(3033..4448)
                     /gene="phoA"
                     /locus_tag="ACHOS5_004966"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP9779640.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4549..4809)
                     /gene="iraP"
                     /locus_tag="ACHOS5_004967"
     CDS             complement(4549..4809)
                     /gene="iraP"
                     /locus_tag="ACHOS5_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP9779641.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT         1142 a         1330 c         1208 g         1225 t
ORIGIN      
        1 caccacttct ccagctccgc agcaaaagcc tggcggtcac gttgtgaaag actatctggt
       61 cctccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
      121 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcggat taagcgccgc cgcgcctttc
      181 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
      241 tgccgaacaa tttcgttatc agcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
      301 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
      361 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
      421 caaattgtca taaagtcatc ctttgttggg taatcctcta ttgtgtcgcg cttttgcctt
      481 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
      541 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
      601 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatatacccc
      661 ctccccggat aaagtcaccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
      721 gcaagaagta gcgcaaatcg ccgacatcat ttttgccgcc gttaaaccag gcatcatgat
      781 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
      841 tgcaggtatc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
      901 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
      961 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
     1021 ggaagtgatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
     1081 ctacgtcttt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
     1141 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggc tccgcaaaaa tggtgctgga
     1201 aacgggagaa catccgggga cactgaaaga tatggtctgc tcaccgggag gcaccaccat
     1261 tgaggcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
     1321 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tggcttttgc cggacgtcag
     1381 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtagagcgc caaatctgcc
     1441 gatttcaacc actcgcgata gtgactcatt tgtggattca gcggcgcaac ccccacacta
     1501 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcatgcacc
     1561 cgtaacatgg cggtgatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
     1621 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
     1681 agcgccacaa tcgcttcatc acccacatca tggccccagg tatcgttgat gctcttgaaa
     1741 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
     1801 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat accgtcgcgg
     1861 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tagtcgccgt ctggtagctg
     1921 acccagccaa acagcagagg ataaatgaca ataatgggaa gggagagcca ccattccagc
     1981 ggtgcgctat tgaacgacac ggtgatgccc gtcagctcga gggtgacaag gcaggagacc
     2041 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
     2101 cacataatca tcaacatcgc ggtggaaggc agtgcgttta cgcccattac gcctacccac
     2161 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
     2221 gccctgctcg ctatttgcca ggctaaatgc ggccagacga acgcccagcc gaccaacaac
     2281 agccaccacc agcccggcga cggatgcgaa accagcgttg aagcaatcgg taagaacatg
     2341 ccagccaggc caaccgcgcg gggtagtctg acgcgacggg caaagcgcag cccggaccgc
     2401 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
     2461 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
     2521 acggggctaa aggcagaagt atgaaatttc gggtgagcca ttttcgtagc tcacccgtta
     2581 gcactatcag gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat
     2641 cccctttcag agattgttgt gttgcttgat tattacattc gcgcatcttt tgctgctgtg
     2701 gcgtcaaact tttttcacca ggcgcagatt tgctgttctt caggcaatca ctcatgtagg
     2761 tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacaa gaggtcatac
     2821 gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac
     2881 cgaaaagcaa ggtaaccagt aatgtaattt tcatcgcacc atccctcttc atgttttaac
     2941 catgagcgta tgcgcccgtg atctgtcatt aagtctggtt gctaacagca aaaaaaccac
     3001 ccggcagcga aaattcactg ccgggcgcgg ttttatttca gccccagggc ggctttcatg
     3061 gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac
     3121 gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcgga gttcccgtaa
     3181 ctcatcacca tcactgcgcc atctttggta tttagcgcct gggtgaggcc cggagctttg
     3241 gtatctggcg caactatctg gctggcatgg gcgtgatcag cggtgactat gaccaacgtg
     3301 ttaccatcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
     3361 tcgccaattt gcccacaagg attcgcagcg tgatcctgtt tatcgattga cgcaccttca
     3421 acttgcagga aaaagccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
     3481 tgcgccaggg tcggtacact gtcattacgt tgcggattag gcgtacaggt gactgcgggc
     3541 ttatctatat taccgtggta cgttgctttc ggtccttgcc agcgcactgg catattgccg
     3601 tcagcaaaca gtcctaatag gggtttttgc tgattcgctt ccgtcaccga attcagtgag
     3661 gcagcatcgc tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
     3721 tgccattcac cggcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaaca
     3781 tcggcacggg cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
     3841 ttacccggac atttttcact ggtcgcactc ggaccgtagc atttgcgcga ggtcacatgc
     3901 gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
     3961 agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
     4021 cccagcgcgc cgttataggt tttgacacca gttgaccagg cggttgctga tgcagccgag
     4081 tcggtgacgt agtccggttt gccggttttt ttattcagcg catagtgagt gtattgcccg
     4141 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattgcgt
     4201 gcggcagtaa tttccgagtc ccccatccca tcgccaatca gcaaaataat attttttgca
     4261 ggtttatcgc taagagaatc acgcagagcg gcggtctgat cacccgttaa gcggcgagca
     4321 ccgccgggtg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
     4381 gtccgggctt ttgtcacagg ggtaaacagt aacggtaaga gtgccagtgc aatagtgctt
     4441 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
     4501 agtctcggcc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacgagg
     4561 atgcctcaat aacttcttta cgtaatcgcg cagcagctcc gtatcgtcgt caggaatgct
     4621 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaacc gctgttgatc
     4681 attttgcgcc atattgcgaa gcattgccgt gacgataatc tccaaagctt ctacctgcgc
     4741 gcacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
     4801 atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtttcacaa
     4861 cttcatagtg tctggaagta ttatttttat gacaaaggaa atact
//
LOCUS       DAZKHK010000233         4899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKHK010000233 DAZKHK010000000
VERSION     DAZKHK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.234"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..896)
                     /locus_tag="ACHOS5_004968"
     CDS             complement(<1..896)
                     /locus_tag="ACHOS5_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779642.1"
                     /translation="MYNFITILYDVFSCFGVLAKNQNTRDIRNIKNFSSHQHSLGDMF
                     DELINIIDKEQVLSTEQRKVTFRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMI
                     LALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPAN
                     AHFKDIFDGVYEFIRNIRKNSTPGKTKLTATINTCKETCKHLLYLSNEDKEKTISDLD
                     KIQVACYYLTILLAFERRTSLTSALATLYKMLISEREVSEYECQLLYLTNPIDVMNIL
                     NKYIYYFPNENSPFYTLKIDSA"
     gene            1093..1786
                     /locus_tag="ACHOS5_004969"
                     /pseudo
     CDS             1093..1786
                     /locus_tag="ACHOS5_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2010..2306
                     /locus_tag="ACHOS5_004970"
     CDS             2010..2306
                     /locus_tag="ACHOS5_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9779643.1"
                     /translation="MPDQSGYCWTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            2614..2730
                     /locus_tag="ACHOS5_004971"
     CDS             2614..2730
                     /locus_tag="ACHOS5_004971"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779644.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2949..4382
                     /locus_tag="ACHOS5_004972"
     CDS             2949..4382
                     /locus_tag="ACHOS5_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP9779645.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     TSIACQNQVDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKSPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTMSAMIQPDVYHKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQELFDKRINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIHHSSGEEGDISPYTAEMARELAIWYGNEYMRLSNPL
                     TFDNTAQNETMRLIPEELELFNWIKSYCIEKGSPCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT         1481 a          891 c         1033 g         1494 t
ORIGIN      
        1 aatgcactgt caattttcag tgtataaaat ggtgagttct cattaggaaa gtaatatatg
       61 tatttgttca gtatattcat tacatctatt gggttggtta aatataataa ctggcattca
      121 tattctgaaa cttctctttc gcttatcagc attttatata aagttgccag ggcgcttgtt
      181 aatgaagttc gtctttcgaa agccagtaat atagtgagat aataacatgc aacttgaatt
      241 ttatctaagt cagaaattgt tttttccttg tcttcattac ttaaataaag cagatgttta
      301 caggtttctt tgcatgtgtt gatagttgcg gtaagttttg tttttcctgg tgtactgttt
      361 ttgcgaatat tacgaatgaa ttcatatacg ccatcaaaga tatctttgaa atgcgcattc
      421 gctggtgtgt atccggataa acataactta acgtaatttc gcagatatgt ccttgcagca
      481 tgatatatat catcctcatt atccggtatc tgagtgagaa aagaatgaat atgatagtaa
      541 aatgccatct cattatccgg gcgatatgtg ttcctgatgt cgagtgcgag aatcattggt
      601 acaatgtcat taaaatattt ttgttttatt atttgatgtg ttttgtctgt aaaaacagaa
      661 tagtgcatta gcttaacata gagatcttca tatctcctaa atgtaacttt tcgttgttct
      721 gtactcaata cttgttcttt atcaataatg tttattaatt catcaaacat gtcgcccagt
      781 gaatgttgat gtgaggaaaa atttttaata tttcggatgt cacgggtatt ctggttttta
      841 gccagaacac caaaacatga aaagacatca tacagtatag tgataaaatt atacataaca
      901 ttctcctttt attgcactat ataaaatttt aacattatat cattttgctt ttctgttaac
      961 tggttttcat ttttgaatca atattttctg aacagggtaa tagtacacgt attaacagat
     1021 tatgtcagaa ggccccggtg atgccttctg acgatagatt aatgtggtat tctattggta
     1081 tctggagacc atatggcaac agttacagta cattctcccc gctgtaattc ctataaagtg
     1141 taccattatg atcacagcac ctcagatcat gagcgtttcc agtgctaatc ctgcaaacgt
     1201 gtgtatttca actgaattac atctacgaaa cccgaaaacc aggcattaaa gagcaaattg
     1261 ttgaaatggt tcataacgga gtcggggttc gcgatagctc cagaacgctg aaggtggata
     1321 tcaacaccgt cattctcact ttataaaacg ctcaccacgc aaagtaacca catctccaga
     1381 cgccagtgcc gatgtggcgc ttatctgcga acttgatgaa caatggagtt ttgtcgaaaa
     1441 caaagctcgt cagcagtggc actggtatgc gtataacacc aaatctggag gtgtgctggc
     1501 ttacactttt ggtcctcgca ctgatgaaac atgtcgtgag ttgccggaac tcctgaagcc
     1561 attctccaca ggcatgataa ccagagacaa ccggagcagt tataccagag aaatgccaca
     1621 ggataagcat cttgtcggca agatatttac ccggcgcatt gagcgcaaca atctgacact
     1681 tcgtactcac atcaaacgac cagcccggaa aacgattttc ttttcacgct ccctggaaat
     1741 tcacgaaaag ccattggttc atttatcgaa aacactgtac tactgactgg agtcattacc
     1801 agggcctctt ttgctgtttt tttaccaatg aatgataatg agaatcgaca gcgataagga
     1861 gaaagtaaaa taacaataat gctgttgttt tctatcgtat tgatttttaa ttatttattg
     1921 tctttttgct cagcccattt ttttacgcag acaaatttac ccggggaata caaactgtaa
     1981 cgacagttca ccacactatg ggggataaaa tgccagatca atccggatac tgctggactc
     2041 cttcacgcat cttacggatg cctgaactga gccaattgtt aggtatatct cgttcgacaa
     2101 tttacgaaaa aatgaatccg ttatcgaaat actacgatgc aaccttcccc aggccggtac
     2161 gtcttggtag tggttctgtg ggctggcgtt catcagctat tgacgaatgg cttactttac
     2221 atactgtacc tgccagatca gcagtcaaag gtgttaatga cgaagtgcga catacgcttt
     2281 cttcagatat gaccatgagc agataagttt ttgtcatcac gtgatcacct caggaggaaa
     2341 aagaagcata agaacaattt agccagaagt ttaattgata aacagaattt ccagcattca
     2401 ttgttgaaag agcagctatt gtcgctattg tagcagtaat tgctgctata accgaaacgt
     2461 attacgaaaa aggaagcatt tatgtcttac atcaaaccgg atatgccgta ttcagtatat
     2521 gcgtgaccat caatgatagg gaaggcaata attaaagctc aatacaataa acaagtacct
     2581 ctgactctgg ttgcaatgtc agtattaaca gcgatgtcaa ttgcctgtca gaatcaggtt
     2641 gacgtatgca gccccggaaa tttacgtggg gccgttaata tttacactat ggttctggag
     2701 aaagggaaaa gaccggggga aaggtattag tgaaataatt tctggaggta ttaagaatga
     2761 gaaatatgaa tgatatttcg tggtaaataa tacagtctgc actgaattaa ccttatcggt
     2821 ttattaaaca tcaggtttac aaatgctctg tatttctcag agcatcaggt ctgaatagca
     2881 gctattttcg ctattgtagc gggagttgct gctattttac taacccatta atgaaaagag
     2941 aggcttttat gtgcttactt gcaccagaaa atccttatcc gatatatgca ctaccaccgc
     3001 tggtgagaaa tgcaataatt gaaactcaaa aaaatacaca ggcacctttg gctatggtgg
     3061 caacatccgc attaacagcg acctcaattg cctgtcagaa tcaggttgac gtgtgcagac
     3121 ctggaaattt acgtgggcct gttaatcttt actctctgat tctggctgat tccggtgaaa
     3181 ggaaaacgac cgtggataaa gtgtttatga aagcatttta tctcagggat gaagccctgg
     3241 cggaagaata cgcgaaactg gttgagaatt acagtacaga aaaggaaata tgggagcaaa
     3301 aacaaaaagc gctggaatca aaatttcata aagagattcg tgccggtaaa gattacaagg
     3361 caacagaatc agagcttgaa acgcatctga ataagtctcc tgttccgccg cagatacgtc
     3421 gaacaatttt taatgagaca acgatagagg gaatgttaaa atattactcc gatagcaatc
     3481 gttcttttgc tcttgtatcc agtgaagggg gggtgatttt tgacagccgg gccatgagta
     3541 aactgggaat tattaacact ttgtgggatg gaggttctct tttcatcgac aggaaatcat
     3601 ctcccggaat taatttgaag gagccaagac tgacgatgtc ggcgatgatt cagcctgatg
     3661 tttaccacaa aggtttttgt acgcgaaaaa aagaacttgt gaaaacgtca ggacatcatg
     3721 caaggttttt gatgtgtcaa ccaacatcaa cgcaggggac aaggataata accggagata
     3781 attattcatc acagtatcag gagttatttg ataagaggat caatgaactt attgatgaaa
     3841 gtctggctat gagtggtgaa cgacgttgcc ttcacttttc tcctcaggct gccagaatct
     3901 ggacggatta ttacaacgat gtggaatcga agctgggggg attggggcct ttaagacatt
     3961 gtcgggaata tgctgccaaa aatgcagagt atatggcaag actggcagga cttattcacc
     4021 attcgagcgg tgaagagggg gacatttccc cttacactgc agaaatggcg agagaattag
     4081 caatatggta cggtaatgag tatatgcggt tgtctaatcc gttaactttt gacaacactg
     4141 ctcaaaacga gactatgcga ctcattccag aggagcttga acttttcaac tggataaaaa
     4201 gctattgcat tgagaagggg agcccctgta tgaaaaaaaa tgacatttta cagcgtggtc
     4261 cgaaccgttt ccggaaaaag gataaaatca actggttact ggatttactg tatgaacaaa
     4321 acagagttgt accggttatt gagggaaaaa cgttgtgtgt cgcacctaac tttgacctct
     4381 gaatgactta atcgttgagt aacatataat ctcctctgct acttgcggag gagactaatg
     4441 cctttaccat gtcagcgttg tattagaaaa ctctggtaca ggtcagtcag taaatataac
     4501 tggtgtaacc actatttact attggataga gtgttgctca ggatattgtc ggctttagtc
     4561 tgaggtgagg tgggaccttc attgttttcg tgttcaataa ataatttagg ttaattagta
     4621 ttattaatgg gagttatgtt atctgttatg ttgtctcttt ctatcgtaat gtcactcctc
     4681 tttatgctga gtattctgta tgtgcgtaat gcgtctatcc ccatatacta tagtgtggtg
     4741 gtgtatgggg tttagttatg tgtaaattat gttactacac tcatagcgta atattgagaa
     4801 agttaatcca ctgaatgtat gtctggtttg tatttaacac tagttacgta ccagaacact
     4861 attagagtat attgtcttta gttcgtacgt tatagctcc
//
LOCUS       DAZKHK010000234         4876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKHK010000234 DAZKHK010000000
VERSION     DAZKHK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.235"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..92
                     /gene="ymgI"
                     /locus_tag="ACHOS5_004973"
     CDS             <1..92
                     /gene="ymgI"
                     /locus_tag="ACHOS5_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP9779646.1"
                     /translation="LNNSERSLVKQILIEDEIIVCATYLIPDN"
     gene            94..438
                     /gene="ymgG"
                     /locus_tag="ACHOS5_004974"
     CDS             94..438
                     /gene="ymgG"
                     /locus_tag="ACHOS5_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP9779647.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            442..777
                     /gene="ymgD"
                     /locus_tag="ACHOS5_004975"
     CDS             442..777
                     /gene="ymgD"
                     /locus_tag="ACHOS5_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP9779648.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(836..1431)
                     /locus_tag="ACHOS5_004976"
                     /pseudo
     CDS             complement(836..1431)
                     /locus_tag="ACHOS5_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1614..1871)
                     /locus_tag="ACHOS5_004977"
     CDS             complement(1614..1871)
                     /locus_tag="ACHOS5_004977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779649.1"
                     /translation="MENNTSLETTDKTNIVTYGKNAVGVLACSSPGESRTCVDAVDDE
                     VCDSNSYEVISRADLKMNGGSITTNGINSYGAYANGEKSIY"
     gene            complement(2173..2352)
                     /locus_tag="ACHOS5_004978"
     CDS             complement(2173..2352)
                     /locus_tag="ACHOS5_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9779650.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYVTQLLDNYSIISYITDEESPIE
                     IKNNTSTSNGEYLIT"
     gene            complement(2750..2968)
                     /gene="ymgF"
                     /locus_tag="ACHOS5_004979"
     CDS             complement(2750..2968)
                     /gene="ymgF"
                     /locus_tag="ACHOS5_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP9779651.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
     gene            complement(3100..3993)
                     /locus_tag="ACHOS5_004980"
                     /pseudo
     CDS             complement(3100..3993)
                     /locus_tag="ACHOS5_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
     gene            complement(4291..4539)
                     /gene="ymgC"
                     /locus_tag="ACHOS5_004981"
     CDS             complement(4291..4539)
                     /gene="ymgC"
                     /locus_tag="ACHOS5_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP9779652.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLK
                     DETSVILSDLYLQILQHIEMHKTTLTDIIINDRESVLS"
     gene            complement(4652..>4876)
                     /gene="ariR"
                     /locus_tag="ACHOS5_004982"
                     /gene_synonym="ymgB"
     CDS             complement(4652..>4876)
                     /gene="ariR"
                     /locus_tag="ACHOS5_004982"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP9779653.1"
                     /translation="ALSSYFRSSGNLLEEESAVLGQAVTNLMLSGDNVNNKNIILSLI
                     HSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT         1468 a         1058 c          967 g         1383 t
ORIGIN      
        1 atctgaataa tagcgagcgt tctttagtta agcagatctt aattgaagac gaaattattg
       61 tgtgtgcaac ttacttaata ccggacaact aagttgaaga aaaaaatact cgccttcggg
      121 cttatctcag cattgttctg ctccacgcca gcaatggcgg atatgaatcg caccacgaaa
      181 ggtgcattgc taggtgcagg cgttggttta cttactggca atggtgttaa tggcgtactg
      241 aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag aaaaaggccg cgacggtaaa
      301 aatgcgcgta aaggtgcaaa agtgggtgcc gccgttggtg ctgtcactgg cgttctgacc
      361 ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg gcggtacggg tggtgcaatt
      421 ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg ctttactggc gggtctgttt
      481 gttttcgcac caatgacctg ggcgcaggac tacaatatta agaacggttt gccatcagaa
      541 acctatatta cttgcgcaga agccaacgaa atggcaaaaa ctgacagcgc acaggttgca
      601 gaaattgttg cggtaatggg taatgccagc gttgccagcc gtgatttaaa aattgagcaa
      661 tctccggaac tgagcgccaa agtggtagaa aaactgaatc aggtttgcgc taaagatccg
      721 caaatgctgt tgatcacggc aattgatgac accatgcgtg ctatcggcaa aaaataatat
      781 ttcatatgtt accgcgtcga tgaattcgac gcggtttttt agctaacggg caggatcaga
      841 acttatattt caggcccacc atcattgcgg tgtcattgta gccattatca cccagctgca
      901 cgcccacatt accccagacg ctggccgccg gattcagatt gccatttacg ccatgtttca
      961 tttcccccag attacgcgcc ccgtcttgtt caactttcac accgtttatt gagacagcaa
     1021 aatctttact gttatggatc ccgttcattt caacaaaagg ttcaaagtcg tcaccgtgcg
     1081 gaccgctgtt ttctcctgag tacgaataaa ggtgtggaaa ccaagtcggg tctggatatt
     1141 gttatttgcg tcattctcaa tacgtgagcc attggcttcc gtgtgcacat tatgatcgac
     1201 gcccatccag atggcctgtg ccgaggtcgt tctcaactgt ccgttgctgt cacgccaggc
     1261 attatgcccg ccaatttgac gtagccaaaa tcggcttgaa cgctcctgtt cagttacagg
     1321 atcgatatag cgcgtttcac ctgcacgatc gttcagacgc ataacaaaaa gagtgttagc
     1381 ggctgccagg ttggcgacgt aactgccagc ttcgggtcgc aggacggggg tagacgtttc
     1441 tgttgattct gaagtgacat tggaaaaaaa taactctcta ccattgtata tttttgcaat
     1501 gggggcttta gtgccatttg ttgtaataga acttttttat atcaatgtta ccttgtcaaa
     1561 tagcaactgc ataacttcca tcagccacag tttcaagtac cacataatct aaattaatat
     1621 atgctttttt ccccattagc ataagcacca tagctattaa taccattagt tgttatggaa
     1681 ccaccattca tttttaaatc agcacggcta ataacttcgt aactgttaga atcacaaact
     1741 tcatcatcta cagcatcgac acatgttcga gactctccag gacttgaaca tgcaagaact
     1801 cctactgcat ttttcccata ggtaacaatg ttcgttttat ctgtggtttc aagactggtg
     1861 ttattttcca cagtctcaga atcattaacc tgacttaaaa gtcccgctga atatgccccc
     1921 tcgtctcgac tgaattatca ccattcataa cgatggtgca accaaattca ctgactactg
     1981 cgctgggcta ttatccctcc attttctttg tcagatacgc tttcagaggt cttaatattg
     2041 ctatcgctta tatataatac tttgttttga ctatcaacag aaataccata agatccatca
     2101 ccactagtca tcacactggc attatttata tccagttaca ccgtcatcca ctttcacagc
     2161 atggctttcg tcttaagtga ttagatactc cccattactt gtagaagtat tatttttgat
     2221 ttcaatcggt gattcttcat cggttatata ggaaattata ctataattat ccaacaattg
     2281 cgttacatat gcattaccca ccgccagagc aataagtcct aaactaaatc cggggagttt
     2341 tttcagtttc atagattaac catccattca ttggattaac aaacattcct ggtcaaataa
     2401 cctatgagtt cagattacca aaacggaagg caaagataaa aacaaaagca caagaaattt
     2461 aaatagataa acagcgacaa tatttaaatg ataaataagc cagcattatc atttttagct
     2521 tattttcatg acattgatca ctttggggta actattttgt ttaacaaata actcttcgac
     2581 atcaaaatta tcaacagttg ccatggaaat tatttcactg atatataaaa caactgtgat
     2641 aacaccaggg ttgacaacta tttgtgcaat aattcctttc cttcaatgat ccaggggttc
     2701 actgccagca acccattcag ccgcatactg gcagtgagaa actgaaagat tagtgctgat
     2761 ccctgtggct gacgagtctt tcccttaatc gattaaccag attgatcgcc agccaaacaa
     2821 gggcattaag caaatttatc gtcacaagca cttctcccag cccggtgctg ggttttgcaa
     2881 taaggatgac caggagagtg atcagcataa atgcaatgga aaagatgata tagagagtga
     2941 aataatccag attattacta ttgttcatgc tactaatggc aatccagtca aaactttcac
     3001 acaattatag caaacaaaag aatgaacaat agtaacaaga ggcaacgagc taattagctg
     3061 tctataatta caccgcacca tggccttgct gatcaatttt cactcaacca caaccttcac
     3121 cttcggttta gaaaggataa ttttgaccag gtcgatgtat gtaacaggtt tatagaaata
     3181 ataaccctgc tgaaatgtga tattattttg gttcagatag tcaaattgtt ctttcgtttc
     3241 gacaccttca gcgacgatac tgatcgaaag tttacgcgcc agatccaata cacaatccag
     3301 aattcgggtt gatgctgggt ctgcgttgac gcggccaacg aaactgtgat caattttgat
     3361 ataatcaata tgcaggtcat gcagataaga aagccctgag taaccagtac cgaaatcatc
     3421 cagcgcgatg acaaaaccat tttcatgcag aatgttcaac cgctgaacca gactttcatc
     3481 aacatttaat ggctcacgct cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa
     3541 actgtcgcgg taatttaagc actcgtcgac aaacgtcggc gaaataatat gcgaggcgct
     3601 aaaattaatc ccaatatgaa aaccttccgg cagtttactc gcgatagcgt tcatctgtct
     3661 ggcaacctga ttcatcaggc tttgcgtaag cggaacgatt aatccgcatt tttcagcaag
     3721 tggaataaat gccgcgggtg atatatatcc accgtgaggt tgtttccagc gggctaacac
     3781 ctcaactccc cgcaatgtcc cttcccgacc atttaccaca ggttggtaaa aagggacgat
     3841 ctccccatta tttatagccc gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata
     3901 cctatcaagc aggaaggcag cggcgcaggc gattaacaga ataaatatca aaatcccaaa
     3961 gcctttatcg ataagtctgt agaagctaaa gagacgttca ttcgctcgcg ctggggtcta
     4021 cagagagcat ggtagagagc atcacaaata tccacgccga agataaaatt ttctccaact
     4081 tcatctagcc tattaatttt ttagcacaat gtaggcagaa ttaaacaaat taggcgggat
     4141 atcaggcgtc aagaatggaa aacgagcgct ctccattcct gtttgccctc tgatattgcg
     4201 gatatattaa gaaagcataa cgcctgaaat gctcactttg catcagcata gtgatacagc
     4261 tgatgtttat tcaaaaacct tactcaagtt ttaagagagc acggattccc tgtcattaat
     4321 aatgatatcc gttagtgtag tcttatgcat ttcaatgtgc tgcaagattt gcagatagag
     4381 atcagaaaga ataacactgg tctcatcttt taaacgcaag cgtaaagcct gagcaataat
     4441 atgactatca acatatccat gtgtcatgaa cacctccctc tcgagatttt taaacaaagc
     4501 acattgaaaa ataaatctct ctgggattga attattcatg tcggcctcgc aggaatagtg
     4561 atccaataaa attatgctgt gtatagtcta aagcgacaga caaaaataag atgaatgagt
     4621 tttctataaa cttatacttt ataattaaaa gttacatatc atcagctgtg tatcgcaaca
     4681 cgatttccag tgtttttcta atcacatcag ctttgagaat atcacttgtt gtttccaggg
     4741 agtgtatcag acttaagata atatttttat tattaacatt atcgcctgaa agcattaaat
     4801 tggtcacagc ctgccctaac acagcggact cttcttctaa caaattacct gaactacgaa
     4861 agtaacttga taacgc
//
LOCUS       DAZKHK010000235         4834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKHK010000235 DAZKHK010000000
VERSION     DAZKHK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.236"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1016)
                     /locus_tag="ACHOS5_004983"
     CDS             complement(<1..1016)
                     /locus_tag="ACHOS5_004983"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFP9779654.1"
                     /translation="MQSVSKSIYRGKLLGSLPAVGEIHKEIAVSEETVTWLSLQREII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVEYAKRLSVLQNKYLSQMYIVMRTYFEKPRTR
                     KGWKGIMHEPDLNGSYNVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAVGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAILAAREQHVVYMTSLTKC
                     ISTLLTDGNPHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     ERQISVAREVIDNRKKMPGYVAGIMLESFLQGGKQSDSLPREYGQSVTDECLSWQQTE
                     QLLST"
     gene            1223..1458
                     /locus_tag="ACHOS5_004984"
                     /pseudo
     CDS             1223..1458
                     /locus_tag="ACHOS5_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            1565..3187
                     /locus_tag="ACHOS5_004985"
                     /pseudo
     CDS             1565..3187
                     /locus_tag="ACHOS5_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            3237..3380
                     /locus_tag="ACHOS5_004986"
                     /pseudo
     CDS             3237..3380
                     /locus_tag="ACHOS5_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3535..3745)
                     /locus_tag="ACHOS5_004987"
                     /pseudo
     CDS             complement(3535..3745)
                     /locus_tag="ACHOS5_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(3888..4451)
                     /locus_tag="ACHOS5_004988"
     CDS             complement(3888..4451)
                     /locus_tag="ACHOS5_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9779655.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHKKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSEVDGLRYALSYLAK
                     QDQKEHGIILMRSRLPEKSNRGRPRHN"
BASE COUNT         1333 a         1153 c         1057 g         1291 t
ORIGIN      
        1 gttgataata attgttctgt ctgttgccat gaaagacact catcagtaac agactgacca
       61 tactctcgcg gaaggctgtc tgattgtttt ccaccttgta aaaagctttc cagcataatt
      121 ccagctacat atcccggcat ctttttccga ttatcaatga cttcacgagc aacagagatt
      181 tgccgttcag ccactttacc actattacca tggctacagt caattatcag acgatgattg
      241 ataccttcat catgcattaa tttcactgcc ttcgttatat ccgacaaacc atagttcggc
      301 tctctaccac cacgtaatat gagatgtcca tgtggattgc catcggttaa tagggtagat
      361 atacattttg ttaaagatgt catatataca acatgttgtt cacgggctgc aagaatagca
      421 tcaatagcta agttaatatt tccatcagtg ctgtttttaa atccaacagg acaatgtaaa
      481 ccagacgcaa gttgacgatg cgtttgtgat tcagtagttc tggcaccaac agccccccaa
      541 catattaaat cagcaatata gggagtaagg aaaggatcga ggaattctgt agcggttgcg
      601 acacgcattg ttgtgattga ggacaaacac tggcgagcat atcgtatccc tttctcaaca
      661 ttatagcttc catttagatc aggctcgtgc attattcctt tccagccttt acgagttcga
      721 ggtttctcaa aataagtgcg cattacaatg tacatttgcg ataagtactt attctgtaat
      781 acggacaatc gtttcgcata ttcaacggca gcctgaacgt catggattga gcatggacca
      841 acaatcacaa gcaagcgagg atcttttccc agcaaaatat tcgctataat ctctcgttgc
      901 agagagagcc atgttacggt ttcttctgat actgcgattt ctttatgaat ctcgcccaca
      961 gcaggtaaac tgccaagcaa cttccctcgg tagattgatt tactgactga ctgcattgta
     1021 tctcctgaaa gttctattct taaccatctg ttgcacatac taattgataa tgataatcat
     1081 ttaatacatg tttgacaata caggatgttc cgtaaccatt tctgcgataa ctcacgttaa
     1141 gccttttcac cttgaaatca cttgttttcc gtaaggtcct acagttgaag aaaattgaat
     1201 ctataaatct gtgatttctt gcgtgacaaa ctcgcacgat acgctttttg gattacctga
     1261 actttaattc cccgatgagc actctcctgc gaaaatattg catttgccag agctcagcgg
     1321 cggtctcata accgtcttgg ttttgccatt cagcttgcac ttgtccgtgc tctcggtcgc
     1381 acactgcgta cagaaaagct ccccccgaag gctgttatca caatagtggc ggaacaactg
     1441 ggtattgacc ttgccgtttt aaagcttaat gtcacgaacg gtgcaaaagt ggttcttccg
     1501 caacctgagt ggaagaaaat ggatgaaact ttctggtgaa caaagatcta agctgtttta
     1561 agttttgggc aactcaatgc actctcttaa gacacttcta cagttacctt gcggatggcg
     1621 atgcagtcga caaattatta gctctgacgg tataaccctc catctccacg gaaaacgaaa
     1681 aactgcacaa tgtcctgaat gctttaagcg tagcgactct gttcatagtt gtcgtcggcg
     1741 ccggatacag catctaccct gctaagggca tacgctatgg cttgtatttt ccgtccgcca
     1801 ctggtactgc cgtaacccgg cttgttcacg taaaattttt gccgagtcgc ttgctccctt
     1861 cgccagttca caccagcagt cttcacaggc gttacaaaat ttacaacgtc aactgggatt
     1921 aatagccgga ggtgaggccg ggaaacgggc tgcaacggca gtgggtctcc gttgcagtgc
     1981 tgatactctt cttcgcaggg ttatcaatac cccggagacg aaacagtcag gcgcgcctca
     2041 tgtcggtatt gatgagtggg cgtggcatcg gggccaccgt tacagtacgt taatcgtcaa
     2101 tcttgatact caccgtcccc tctcgtcctg cttcccggcc gtgatcagcg tacgctggcg
     2161 acctggttca gaaaatatcc ggaaatacag gttgtctcgc gtgatcgcag tggagtctat
     2221 acgacagcag cacgtgaagg tgcacctcag gccagacagg tggccgatcg atggcacctg
     2281 ctaaaaaata ttggcgatgc gcctgaacga atgatgtaca gacatatgcc tctgatacgt
     2341 cttgttgcca gtgagttgtc accaaagaaa tcacctgatc cagaaccatc tgtgcctgca
     2401 gcatcgctcc gtcgtccgga acacctaaaa cagcaaaccc gcaaaaaacg gcatcagcgt
     2461 tggacagagg ttatggccct gcataacaag ggatgtagtt tcagggaaat atcccgtatt
     2521 acaggcctgt cgcgtgtgac agtcagtcgc tgagtacgct caggaacatt ccctgaaatg
     2581 tcaacccgac ctccaaagcg agggcttttg gacccatgga gggagtggtt aaaagagcaa
     2641 cgagaaagtg ggaattataa cgccagccgg ataatggcgg gaaatggtgg tccgggggtt
     2701 tacaggcagt gaaaccattg tcagggatac tgttgccaaa tggcgtaaag gctggatccc
     2761 accggttact actgccgtca gacttccttc agtgtcccgg gtaaggcgct ggttgatgcc
     2821 ctggagaata atcagggatg aagaaaatta tgcttcccga tttattagtc tgatgtgtga
     2881 aaaagaaccg gggctgaaaa tagcgcagca actggcactc gagttctacc gtattctgaa
     2941 aacccaaaat aaatcacagc taagtagctg gttcactcga gtccacgaaa gcggttcagc
     3001 agaatttcgg cgcgtggcgg cggggatgga agctgatgct gcagctatat gtgaggcaat
     3061 cagtagccgc tggagtaatg gtgttgtcga aggtcatgta aaccgcctga agatgctaaa
     3121 acgccagatg tatggtagag ccggatttga actactcagg cagagggtca tgagtccact
     3181 ggcatgaaaa tctccaccca agttgcggaa gaaccacttt tgcaccgtta gtgacaggaa
     3241 gaagaaatta cctcttcttc ggttctgaca acggtggaga aagtgctgcg atcatctaca
     3301 gtctgctggt tacctgcaaa ctgaatgggg tggagccgga ggactggttg cgcgaagtga
     3361 tcgtaaagat caataactgg tgctgccctg taacttctct gctgcaaaat aatccttccg
     3421 ctacgaactg ctcggggcgc ttacgtatat ccggcctgtt catgaagtta ttgcttctgg
     3481 ccttgatgag acattcgcgc gctccgacct tatcggttac ttggccttac tttattttcc
     3541 gctagtttta ctttcccatc gaaggcccca tacatcacca cgcccacgaa aaaaccgacg
     3601 aaataacgca actttctcat ccgtggataa tacggaaacg cactgcacag gactctgcgg
     3661 tttacgtcgc cattcaatcc cataagcttc aagtgatgtc ttccctcagt gctgctaatt
     3721 catcactgtc agatttatga accatattcg ttacctgtat ccgaaccact gaagcaccac
     3781 agcagaaatt cgggtcccct acactgatta cagcgccccc tctactgcct attaactcaa
     3841 gcacctccca acaacttacg gaaacttaat tctaatacag ctgtaattca gttgtgtcgt
     3901 ggtcggccgc ggttactttt ttcgggaagt ctgctacgca tcagaataat accgtgttct
     3961 ttctgatcct gtttagccag gtagctaagg gcgtaacgta aaccgtctac ctcagactta
     4021 tcactatagt gaatcacgtg atcgatacgc accgggtatt tgtctttagc ccggcaaagg
     4081 tgaaaataac cctccccttc cgtgatccgc ctccagatat ctcccagttg cctggatatc
     4141 cgataagatt tcttgtgacg ttgcccatcc aggtagccga taaaatgaat atgaagtccc
     4201 ttgtttgacg tatattccat tacccagtaa tatcccgcca gcatcgtttg ggtttcacta
     4261 agtaagcggt gcatttcaat acacatactg tgcctacagg aatgaccaaa actgggcgtg
     4321 tctttcctgt aggcaaaatc aattctgaaa ggtaacagtt tagaaaaacg ttgaaacaga
     4381 ccttccatgt gttcattcac gtctttcaga atcatgaagt ccatttcgta attgggatta
     4441 gcattataca ttttatagtc cttactttat aaaactgaca ggaaagtgga attacataaa
     4501 tactgagagt acagaaacgc cgtaccgcgg cattaataaa cgaggtaacc agcagcacag
     4561 acaacctgat aaaactgttt cgtattacct cattacagta atagggtaat gattcagaat
     4621 acagtaagac tctcttaaac tgaggaccag aacgaattac aataaaaaac acacctcata
     4681 taattgggtt taagaaagca ccattcaatt acttacagga aataatctga tcttcacaaa
     4741 agtcagtacc gggtagccac aggcaacatc gctattaata ttgccgtacc attaagtaaa
     4801 aacagagact ctgcagagag agcctacaga tatt
//
LOCUS       DAZKHK010000236         4827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKHK010000236 DAZKHK010000000
VERSION     DAZKHK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.237"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..333
                     /locus_tag="ACHOS5_004989"
                     /pseudo
     CDS             42..333
                     /locus_tag="ACHOS5_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            330..680
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004990"
     CDS             330..680
                     /gene="tnpB"
                     /locus_tag="ACHOS5_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9779656.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDETPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTFRLNSLTML"
     gene            711..2304
                     /locus_tag="ACHOS5_004991"
                     /pseudo
     CDS             711..2304
                     /locus_tag="ACHOS5_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2452..3440
                     /locus_tag="ACHOS5_004992"
                     /pseudo
     CDS             2452..3440
                     /locus_tag="ACHOS5_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3519..4011)
                     /locus_tag="ACHOS5_004993"
                     /pseudo
     CDS             complement(3519..4011)
                     /locus_tag="ACHOS5_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
     gene            complement(4008..4385)
                     /locus_tag="ACHOS5_004994"
     CDS             complement(4008..4385)
                     /locus_tag="ACHOS5_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9779657.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSACTRSQLINSIDILRARRATG
                     LMTRSNYRMVNDIIRGEHSEAKR"
     gene            complement(4432..4806)
                     /locus_tag="ACHOS5_004995"
     CDS             complement(4432..4806)
                     /locus_tag="ACHOS5_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9779658.1"
                     /translation="MSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTLAPATTS"
BASE COUNT         1212 a         1213 c         1338 g         1064 t
ORIGIN      
        1 actaaattag agctccttct aaattagacg gagttctatt gatggataaa cctacagact
       61 ggcgctctgg gacgcgccgg atattttcta atgaatttaa gcttcatatg gttgaactgg
      121 cttcgaaacc aaatgccaat gttgcacaac tggcccggga acatggcgtt gataacaacc
      181 tgatttttaa atggctacgt ctctggcaaa gagaaggacg tatttctcgt agaatgcctc
      241 caactattcc attacggtaa aatgacactg ggaaatcctt caccagagct gctcacggtg
      301 ttgatccgtg aactgaccgg gaggggaaga tgatctcact cccatcaggt acccgcatct
      361 ggctcgtggc cggcgttacc gatatgcgta aatccttcaa cggtctggga gaacaggtac
      421 aacatgtgct ggatgagacc cccttctcag gtcacctgtt tatctttcgt ggccgacggg
      481 gtgacactgt taaaatactc tgggccgatg ctgatggtct gtgcctgttc accaaacgcc
      541 tggaggaagg ccagtttatc tggcctgcgg tgcgtgacgg taaggtatcc attacccgct
      601 cgcaactggc aatgctcctc gataagctgg actggcgtca gccaaaaaca ttccgcctta
      661 actcactgac aatgttgtaa aaaatgcctg gccgcattat aaaaacggcc atgagtcaga
      721 aatacctcat tcgcattgca gaactggaaa gccagctccg tcagaaagac cagcaactga
      781 gtctggttga agagacgaag accttcctgc gctctgcact ggcacgtgcc gaagaaaaga
      841 tcgaagaaga tgaacgggaa atagaacatc tgcgggctca gatagaaaaa ctgcgccgga
      901 tgctgttcgg tacctgttct gaaaaactgc gtcgtgaagt tgaactggct gaggccctgc
      961 tgaaacaacg cgaacaggac agcgatcgtt acagtgggcg ggaagacgat ccacaggttc
     1021 cccgccagtt gcggcagtcg cgccatcgtc gtccgttccc ggcacacctt ccccgtgaaa
     1081 tacaccgtct ggagtctgaa gaaagctgtt gcccggagtg tggcggtgag ctggattatc
     1141 tgggggaagt cagtgcagaa caactggaac tggtgagcag cgctctgaaa gtgatccgca
     1201 cagaacgggt aaaaaaagcc tgtacaaaat gtgaatgcat cgttgaagca ccggcgccgt
     1261 cccgccgcga tagagcgtgg tatcgcgggc cccggattac ttgcccgcgt gtttacggga
     1321 aaatactgcg aacacctgcc actgtatcgt cagagtgaaa tctttgcccg acagggtgtc
     1381 gaactgagcc gtgcattact ctccaactgg gttgacgcgt gctgccagtt aatgacgccg
     1441 ctgaatgatg ccctgtatcg ttatgtgaag aatacccgca agcttcacac tgacgacaca
     1501 ccggtaaagg tactggcacc gggcctgaaa aagacgaaaa cagggcgcat ctggacgtat
     1561 gtccgggatg atcgcaatgc gggttcgtca tctcctccgg cggtctggtt cgcgtactca
     1621 ccgaacaggc aggggaaaca cccggagcaa cacctccgcc ccttccgggg tatcctgcag
     1681 gcggatgcgt tcacaggtta cgacaggctg ttcagtgcag aacgcgaagg tggtgcgctg
     1741 acagaagttg cgtgctgtgc tcatgcccgg agaaaaatcc acgatgtata catcagcagc
     1801 aaaagtgcga tggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat
     1861 gaaatacggg gattaccaga gtcagagcgt cttgcagtca ggcagcagcg aagcaaagcg
     1921 ttactgacgt cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc
     1981 agactgggcg aagcgttcag ctatgtactg aatcagtggg acgccctctg ttattaccgt
     2041 gatgacggtc tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt
     2101 ctcggaaaga aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggagcactg
     2161 ctgtacgggc tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc
     2221 catattctga gcgtactgcc ggaatggccc tccaaccgtg ttgacgaact cctgccatgg
     2281 aacgtagttc tcaccaataa ataagcgtca atacggtgct ccgttgacgc ttactattgt
     2341 tctgcagcta aatagctgcg cggaatagta gatcactttg agggaactca gcccggattg
     2401 tgcgatctga tcaatcgcca aatcaaaaca aatcaccaac cggactgagc aatgccgatc
     2461 atagcaccaa tttcccgtga cgaacgacgc ctgatgcaga aagccatcca taaaacacac
     2521 gataaaaatt atgcccgcag actgactgcc atgctgatgc tgcaccgggg cgaccgtgtc
     2581 agcgacgttg ccagaacgct ctgctgcgcc cgttcctctg ttggacgctg gattaactgg
     2641 ttcacgcagt cgggtgttga gggactgaaa tcattacctg ccgggcgtgc ccgtcgctgg
     2701 ccgtttgagc atatctgcac actgttacgt gagctggtaa aacattctcc cggcgacttt
     2761 ggctaccagc gttcacgctg gagtacagaa ctgctggcaa taaaaatcaa tgagataacc
     2821 ggttgccagt taaatgccgg aaccgttcgc cgctggttgc cgtctgcggg gattgtgtgg
     2881 cgaagggctg cgccaactct gcgtatccgt gacccgcata aagatgaaaa gatggcagca
     2941 atccataaag cactggacga atgctgcgca gagcatccgg tcttttatga agatgaagtg
     3001 gatatccatc ttaatcccaa aatcggtgcg gactggcaac tgcgcggaca gcaaaaacgg
     3061 gtggtcacgc cgggacaggt aaagccagct atgtgggcgg caacagcaaa agttcggcgc
     3121 tgttcatcag cctgctgaaa cggcttaaag cgacataccg tcgggcgaaa accatcacgc
     3181 tgatcgtgga caactacatt atccacaaaa gccgggaaac acagagctgg ctgaaggaga
     3241 acccgaagtt cagggtcatt tatcagccgg tttactcgcc atgggtgaat catgttgaac
     3301 ggctatggca ggcacttcac gacacaataa cgcgtaatca tcagtgccgc tcaatgtggc
     3361 aactgttgaa aaaagttcgc cattttatgg aaaccgttag cccattcccc ggaggcaaac
     3421 atgggctggc aaaagtgtag cggtattaag cgcagctatt taggcaatca gaatttccag
     3481 cgccgtttcc gtgattaatg atgtcatgtc gttcctagtt accagtcgaa agtgggcagg
     3541 cccggcagac atcctgcact ggcagccaga tgaatgagac cgacgccata acaccggatg
     3601 agagtgacaa ccagacgacg gaacgttttt gacaggccaa gacgtttaag ccgcagaacc
     3661 ggatacgacc acgcatcagt acgtaacaga taaccggtac ctgtaaaatg aatccattct
     3721 gattcgccga agtcattcgt ctgatgtgac agtgagtaca gccaggcgtt gtccttttcc
     3781 gtgatatgtg cggtactgca acgaacgtat gtcggtaagg gcagtaaacg gtggtggaga
     3841 gtcggttgtc tgctgttcgg gagcatccac cacgcggagt gaataaccgt tttcagcgac
     3901 cttattattc aactcagcgg ggtcaatacc atcgacgtca acgacaattc gccccatgtt
     3961 cagtgcctgc acgttaacgc tgtctgcttc gagcgtcaga gtcagtttca tcgtttcgcc
     4021 tccgaatgtt caccccggat gatgtcgttc accattctgt agttgctgcg ggtcatcagg
     4081 ccggttgccc ggcgggcccg gaggatatca atactgttta ttaactgaga acgggtacaa
     4141 gcgctgaatc ttggctggtc ggtacgtacc agtgcgtatt tttcaacgag aaagttcacc
     4201 gcatcgcaca gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     4261 ggtgtgtcat tcagtgtgag gccgtaatgc tggtccaaca gttgggtgag cagtgtctgc
     4321 cagatggtga cgggagacgg gcagcgcgat gcctcccgta cgtgtgtgtc aggtaatgtt
     4381 ttcatcttaa gttctctgta actggtagtg ctaaaggtga gatattcggg gttatgaggt
     4441 ggttgcgggt gccagtgtcg gataaacagc catataaacg taaccacagc tgccgagggt
     4501 gtcggcttcg caggtcagcc ctctggcata cagcgtgacg gtatgctgat ggcggggatt
     4561 cagttcaccg ctggtgagca tgagttccag ttgtttcatc agcagtggaa aagcctggtc
     4621 cagatggtat gcatccgcgt cgctgaactg ccctctgata ccggcgcggt cggcaaggta
     4681 atgcaactga ttgccctcct gcaccagacg tgccccgaaa cagggcgtca cggtgcaggg
     4741 tagcccccac caggggcggt cgttgttgtc gtcgggataa tttgtttcat ggagcgtgtc
     4801 tgacacgata aagtcctcac agaaaat
//
LOCUS       DAZKHK010000237         4734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKHK010000237 DAZKHK010000000
VERSION     DAZKHK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.238"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            145..1083
                     /gene="tdcA"
                     /locus_tag="ACHOS5_004996"
     CDS             145..1083
                     /gene="tdcA"
                     /locus_tag="ACHOS5_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP9779659.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
     gene            1182..2171
                     /gene="tdcB"
                     /locus_tag="ACHOS5_004997"
     CDS             1182..2171
                     /gene="tdcB"
                     /locus_tag="ACHOS5_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP9779660.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2193..3524
                     /gene="tdcC"
                     /locus_tag="ACHOS5_004998"
     CDS             2193..3524
                     /gene="tdcC"
                     /locus_tag="ACHOS5_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP9779661.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3550..>4734
                     /gene="tdcD"
                     /locus_tag="ACHOS5_004999"
     CDS             3550..>4734
                     /gene="tdcD"
                     /locus_tag="ACHOS5_004999"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP9779662.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDNVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKV"
BASE COUNT         1176 a         1019 c         1156 g         1383 t
ORIGIN      
        1 acttgttgat atttaatggt atttaatttt ggtgacgatg ccatggaaag tattgaaagt
       61 taatttgtga gtggtcgcac atatcctgtt catttcattt tgatacactt catgccgtca
      121 atgaggtaat taacgtaggt cgttatgagc actattcttc ttccgaaaac gcagcacctg
      181 gtagtctttc aggaagttat tagaagtggt tctatcggct cggctgcaaa agagttaggg
      241 ttaactcaac cggccgtcag taaaatcatt aacgatattg aagattattt tggtgtggag
      301 ttagtggtgc ggaaaaatac cggtgtaaca ttaacacctg ccggtcaatt gttactctcc
      361 cgttccgaat ccattacccg tgaaatgaaa aatatggtta atgagataag cggtatgtct
      421 tctgaggcgg tggtggaagt ctcatttggt tttccttcat tgattggttt tacttttatg
      481 tcagggatga tcaacaagtt caaagaggtg ttcccgaaag cgcaggtttc tatgtatgaa
      541 gcgcaactgt cttcgttctt accggcaatc cgcgacggtc gactggattt tgcgattggt
      601 acgttaagcg cagaaatgaa gcttcaggat ttacatgttg agccgctgtt cgagtccgag
      661 tttgtgctgg tagccagtaa gtcccgaaca tgcaccggca ccaccacgct ggagtcgttg
      721 aagaacgaac agtgggtgtt gccacaaacc aatatggggt actacagcga actacttact
      781 acgttacaaa gaaatggcat cagtattgaa aacatcgtta aaaccgactc agtcgtgaca
      841 atttataatc ttgttctcaa tgctgatttc ttaactgtaa ttccttgtga tatgacatca
      901 ccttttggtt ctaatcaatt tattactatt ccggttgaag aaacattacc tgtggcacaa
      961 tatgccgcgg tatggtcgaa aaattatcgt attaaaaaag cagcatcggt tttggtggaa
     1021 ttagccaaag agtattcatc ttataatggg tgtagacgaa ggcaattaat tgaagttgat
     1081 tagttatttg ttttaattta aacataaata atccacctgt ctgttttgca ggtgtcggtt
     1141 acggttacct acatatttaa ttcaggctaa gaggttttat aatgcatatt acatacgatc
     1201 tcccggttgc tattgatgac atcattgaag caaaacaacg actggctggg cgaatttata
     1261 aaacaggtat gcctcgctcc aactatttta gtgaacgttg caaaggtgaa atattcctca
     1321 aattcgaaaa catgcagcgt accggttcat ttaaaattcg tggcgcattt aataaattaa
     1381 gttcactgac tgatgcggaa aaacgtaaag gtgtagtggc ctgctctgcg ggcaaccatg
     1441 cgcaaggggt ttcgctctcc tgcgcgatgc tgggtattga cggtaaagtg gtgatgccaa
     1501 aaggtgcgcc gaaatccaaa gtcgcggcaa cgtgcgacta ctccgcagaa gtcgtgttgc
     1561 atggtgataa cttcaacgac actattgcta aagtgagcga aattgtcgaa atggaaggcc
     1621 gtatttttat cccaccttac gatgatccga aagtgattgc tggccaggga acgattggtc
     1681 tggaaattat ggaagatctc tatgatgtcg ataacgtgat tgtgccaatt ggcggcggcg
     1741 gtttaattgc aggtattgcg gtggcaatta aatctatcaa cccgaccatc cgtgttattg
     1801 gcgtacagtc tgaaaacgtt cacggcatgg cggcttcttt ccactccgga gaaataacta
     1861 cgcaccgaac taccggcacc ctggcggatg gttgtgatgt ctcccgcccg ggtaatttaa
     1921 cttacgaaat cgttcgtgaa ttagtcgatg acatcgtgct ggtcagcgaa gacgaaatca
     1981 gaaacagtat gattgcctta attcagcgca ataaagtcgt caccgaaggc gcaggcgctc
     2041 tggcatgtgc tgcattatta agtggtaaat tagaccaata tattcaaaac agaaaaaccg
     2101 tcagtattat ttccggcggc aatatcgatc tttctcgcgt ctctcaaatc accggtttcg
     2161 ttgacgctta attaattcgt tgaggatagg atatgagtac ttcagatagc attgtatcca
     2221 gccagacaaa acaatcgtcc tggcgtaaat cagataccac atggacgtta ggcttgtttg
     2281 gtacggcaat cggcgccggg gtgctgttct tccctatccg cgcaggtttt ggcggactga
     2341 tcccgattct tctgatgttg gtattagcat accccatcgc gttttattgc caccgggcac
     2401 tggcgcgtct gtgtctttct ggctctaacc cttccggcaa cattacggaa acggtggaag
     2461 agcattttgg taaaactggc ggcgtggtta tcacgttcct gtacttcttc gcgatttgcc
     2521 cactgttgtg gatttatggc gttactatta ccaatacctt tatgacgttc tgggaaaacc
     2581 agctcggctt tgcaccgctg aatcgcggct ttgtggcgct gttcctgttg ctgctgatgg
     2641 ctttcgtcat ctggtttggt aaggatctga tggttaaagt gatgagctat ctggtatggc
     2701 cgtttatcgc cagcctggtg ctgatttctc tttcgctgat cccttactgg aactctgcag
     2761 ttatcgacca ggttgacctc ggttcgctgt cgttaactgg tcatgacggt atcctgatca
     2821 ccgtctggct ggggatttcc atcatggttt tctcctttaa cttctcgcca atcgtctctt
     2881 ccttcgtggt ttctaaacgt gaagagtacg agaaagattt tggtcgcgac ttcaccgagc
     2941 gtaaatgttc acaaatcatc tctcgcgcca gtatgctgat ggttgcagtg gtgatgttct
     3001 ttgcctttag ctgcctgttt acgctgtctc cggcgaatat ggctgaagcg aaagcgcaga
     3061 atattccagt gctttcttat ctggctaacc actttgcgtc catgaccggt accaaaacaa
     3121 cgtttgcgat tacactggaa tatgccgctt ccatcatcgc actcgtggct attttcaaat
     3181 ctttcttcgg tcactatctg gggacgctgg aagggctgaa tggtttgatt ctgaagttcg
     3241 gttataaagg tgacaaaacc aaagtgtcgc tgggtaaact gaatactatc agcatgattt
     3301 tcattatggg ctccacctgg gttgttgcct acgccaaccc gaacatcctc gacctgattg
     3361 aagccatggg cgcaccgatt atcgcatcac tgctgtgcct gttgccgatg tatgccatcc
     3421 gtaaagcgcc gtctctggcg aaataccgtg gtcgtctgga taacgtgttt gttaccgtga
     3481 ttggtctgct gaccatcctg aacatcgtat acaaactgtt ttaatccgta actcaggatg
     3541 agaaaagaga tgaatgaatt tccggttgtt ttggttatta actgtggttc gtcttcgatt
     3601 aagttttccg tactcaatgc cagcgactgt gaggtattaa tgtcaggtat tgccgacggt
     3661 attaactcgg aaaatgcatt cttatccgta aatgggggag agccagcacc gctggctcac
     3721 cacagctacg aaggtgcatt gaaggcaatt gcatttgaac tggaaaaacg gaatttaaat
     3781 gacaatgtgg ccttaattgg ccaccgcatc gctcacggcg gcagtatttt taccgagtcc
     3841 gccattatta ccgatgaagt cattgataat atccgtcgcg tttctccact ggcacccctg
     3901 cataattacg ccaatttaag tggtattgaa tcggcgcagc aattattccc gggcgtaact
     3961 caggtggcgg tatttgatac cagtttccac cagacgatgg ctccggaagc ttatttatac
     4021 ggcctgccgt ggaaatatta tgaagagtta ggtgtacgtc gttatggttt ccacggcacg
     4081 tcgcaccgct atgtttccca gcgcgcgcat tctctgctga atctggcgga agatgactcc
     4141 ggcctggttg tggcgcatct tggcaatggc gcgtcaatct gcgcggttcg caacggtcag
     4201 agtgttgaca cttccatggg aatgacgccg ctggaaggct tgatgatggg tacccgcagt
     4261 ggtgatgtcg acttcggggc gatgtcctgg gtcgccagcc aaaccaacca gagcctgggg
     4321 gacctggaac gcgtagtgaa taaagagtcg ggattattag gtatttccgg cctttcttcg
     4381 gatttacgtg ttctggaaaa agcctggcat gaaggtcacg aacgcgcgca actggcaatt
     4441 aaaacctttg ttcaccgaat tgcccgtcat attgccggac acgctgcttc attacatcgc
     4501 ctggatggaa ttatattcac cggcggaata ggagagaatt caagtttaat tcgtcgtctt
     4561 gtcatggaac atttagctgt attaggcgta gagattgata cagaaatgaa taatcgctct
     4621 aactcttttg gagagcgaat tgtttccagt gaaaatgcgc gtgtcatttg cgccgtaatt
     4681 ccgactaacg aagaaaaaat gattgctttg gatgccattc atttaggcaa agtg
//
LOCUS       DAZKHK010000238         4707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKHK010000238 DAZKHK010000000
VERSION     DAZKHK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.239"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..717)
                     /locus_tag="ACHOS5_005000"
     CDS             complement(<1..717)
                     /locus_tag="ACHOS5_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFP9779663.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPKVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKER
                     EDVMDQIIQEGGLEYATDVII"
     gene            complement(858..2891)
                     /gene="metG"
                     /locus_tag="ACHOS5_005001"
     CDS             complement(858..2891)
                     /gene="metG"
                     /locus_tag="ACHOS5_005001"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9779664.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3023..4132
                     /gene="apbC"
                     /locus_tag="ACHOS5_005002"
     CDS             3023..4132
                     /gene="apbC"
                     /locus_tag="ACHOS5_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP9779665.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4395..4676
                     /locus_tag="ACHOS5_005003"
     CDS             4395..4676
                     /locus_tag="ACHOS5_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP9779666.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
BASE COUNT         1149 a         1164 c         1172 g         1222 t
ORIGIN      
        1 tattattaca tcagtagcat attccagacc gccttcctga ataatttgat ccataacatc
       61 ctcacgctct ttatatcttg tgcatttcat ttcaagtgcc gtgagtacag ccattacggg
      121 tgatggtagc tgtgttgttt tcaggccgtc gatctgactg atcgcctggg cgacggtgtt
      181 ttgccattct aaagaacagg cgctgaagtc aaatgtggct gttttattat cccgttgcga
      241 cacactacag gctaagcgac gtaattgggt caatttgtct gcttgctgaa caggaactcc
      301 gggaaagcgg cgatgactta acgtggtgtg tacaactttg ggcggaaaaa cgacagcaat
      361 atctttcgcc agccacggta aaacagcatc tgtatttttg tcagccgcac gggtttctgc
      421 tacgaaagag ggttgttcct gaataatggt ggataagtcc gctaccttga gagagtccgt
      481 taccgtttgg gaaggttgca aagaattatc cttcgcttgc tccacatacc ccttcttcac
      541 cttctccgca atcagcttca gtcccgcttt ttccgccgct gctgcatccg caaaactttt
      601 tacctggctt tgtccgttgg tgccgacttt accccagttg atgtgcagtt cgtttccctg
      661 ctgctccacc gcccagaatt tatgtgattt ttcgtcctga tagataaagt gtctcattct
      721 gcatccctgt aattttatcc agttgaatgc agatgctacc agtatttatg agggttagag
      781 agagacaaaa tcgcaagaaa acaactttag gaatttataa agaaaaggcg ctgtcgatgc
      841 agcgccttga agggagatta tttcacctga tgacccggtt tagcaccggc atccgggctt
      901 agcaggaaga tatctttccc gccaggacca gcagccatta ccatgccttc ggagataccg
      961 aagcgcattt tacgtggtgc caggttagcc accataatgg tgtgacgacc aattagtgct
     1021 tgcggatccg ggtaagcaga acgaatgccg gagaagacat tgcgtttttc accgccgaga
     1081 tccagcgtca ggcgcagcag tttgtcagaa ccttctacaa actctgcgtt ttcaatcaac
     1141 gccacgcgca ggtcaacttt ggcgaagtcg tcaaaggtga tggtttcctg aatcggatca
     1201 tctgccagtg ggccagttac tggcgcggca gcggctttta cttcctcttt tgatgcttcc
     1261 acgagtgctt caacctgttt catatcgatg cggttataca gagccttgaa tggattcact
     1321 ttatggccca acagcggttg ctggatacca tcccaggtca gttctgtatt gaggaatgct
     1381 tctgcgcgct cggtcagttt cggcagtacc ggtttcaggt aagtcatcag cacgcggaac
     1441 aggttgatgc ccattgagca aatcgcctgc agatcggcat cgcgaccttc ctgcttcgcc
     1501 accacccacg gagcctgctc gtcgacatag cggttagcca ggtcagccag tgccatgatt
     1561 tcacggatag ctttaccaaa ttcgcggctt tcccacgctt caccaatcac ttcagcggca
     1621 tcagtgaagg ttttgtacaa ctgcgggtca gccagctcgc ttgccagcac accgtcaaaa
     1681 cgcttattga taaagccagc gttacgggac gccaggttaa ccactttgtt cacgatatcg
     1741 gcattcactc gctgaacgaa atcctccagg ttgagatcga tatcatcaat gcgcgaagag
     1801 agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa aatgattcag ccaggtgctg
     1861 gctttaataa aggtgccgcg agacttggac attttcgcgc cgttcaccgt cacatagccg
     1921 tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt ccagcatggc aggccagaac
     1981 aggctgtgga agtaaacgat atctttaccg atgaagtgat acagctcggc ggtggaatct
     2041 ttcttccagt attcatcgaa gcttacgctg tcgccgcgct tatcacacag attcttgaaa
     2101 gagcccatat agccaatcgg tgcgtccagc cagacataga aatatttgcc cggcgcgttc
     2161 ggaatttcaa aaccgaaata aggggcgtcg cgggagatat cccactgttg caggccagat
     2221 tcaaaccact cctgcatttt atttgccacc tgctcctgca acgcaccgct gcgggtccat
     2281 gcctgcagca tttcgctgaa agagggcaga tcaaagaaga agtgttcgga atcacgcatt
     2341 accggcgtag caccagaaac taccgatttc ggctcgatca gttcagtcgg gctgtaagtc
     2401 gcgccgcaga cttcacagtt atcgccgtat tgatctggcg ctttacattt agggcaggtg
     2461 ccttttacaa aacggtccgg caggaacatg cctttttccg gatcgtacaa ctgagagata
     2521 gtgcggtttt taataaaacc gttctctttc aggcgagtat agataagctc agacagctgg
     2581 cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc tgatgttaaa gcctgcgaaa
     2641 tcagtctggt gttcctgact catttcgcca atcatctgct ccggtgtgat accaagctgc
     2701 tgtgctttca gcatgatcgg cgtaccgtgg gcatcgtcgg cacagatgaa attaacctcg
     2761 tggccgcgca ttcgctggta acgaacccag acatcagcct ggatgtgctc cagcatatgg
     2821 ccgaggtgga ttgagccgtt agcgtacggc agcgcgcacg tcaccagaat tttcttcgcg
     2881 acttgggtca tagtaggcat tacttcttaa tagtgaaaag ggcttagata gtaccaaatg
     2941 ggaaaatgtt aagtaagtac ttctctatat tcttcaggta aactatcaac tgatggttta
     3001 tctatacaaa aaaggagtcg ggatgaacga acaatcccag gccaaatcac cagaagcctt
     3061 gcgcgcaatg gtcgccggga cactggccaa ttttcagcac ccaaccctga agcataacct
     3121 cacaacgctg aaagcgttgc accatgtcgc ctggatggac gacacgctgc atgtggaact
     3181 ggttatgcca ttcgtctggc atagcgcgtt cgaagagtta aaagagcaat gtagcgccga
     3241 actgctgcgc atcaccggag cgaaagctat cgactggaaa ctgtcgcata acatagccac
     3301 tctgaaacgc gtgaagaatc agccgggaat taacggcgtg aagaacatta tcgcggtcag
     3361 ttccggtaaa ggtggcgtgg gtaaatcctc cacggcggta aacctggcgt tggcactggc
     3421 ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc tacggcccat caatccctac
     3481 catgctgggc gcagaaaatc agcgtccaac ttcgccggac ggtacccata tggcaccgat
     3541 tatgtctcat ggcctggcaa ctaattcaat tggttatctg gtgacagacg acaatgcgat
     3601 ggtgtggcgt ggcccaatgg cgagcaaggc gttaatgcaa atgttgcagg aaaccttgtg
     3661 gccggatctc gactatctgg tgcttgatat gccgccaggt accggtgaca ttcaattgac
     3721 attggcacaa aacattccag taaccggtgc agtcgtggtc accaccccgc aggacatcgc
     3781 gttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa gtcgaggttc cggtgctcgg
     3841 tattgtcgag aacatgagtg tgcatatttg cagtaactgt ggtcaccatg aaccgatttt
     3901 cggtaccggt ggtgcagaga aactggccga gaaataccac acacagctgc tggggcagat
     3961 gccgttacac atctccttac gtgaggatct cgataaaggc acgccaacgg ttatcagccg
     4021 tcctgagagc gaatttaccg ctatctaccg ccagctggct gaccgcgttg cagctcagct
     4081 ctactggcag ggtgaagtca ttccaggcga gatttccttc cgcgcggtct aacgcgtatt
     4141 aaccattctg gttattcttc atacaccgca gagatgttac attgatgcgg tgtatttgtt
     4201 tttgcaacat taatgaacac ttatttaatg ctgcgaatat tctgcggacc ttcgtccgaa
     4261 actctacaaa gtcgattgat cgtaccggcg atgcttcatt atcatccttc gtggctgtgt
     4321 agtggatgta cagctatagt ttgttatgca ctcattcttt ccaggcataa cctttactac
     4381 agggataacg ttcaatgaat aagtactggc tgtcaggaat tatttttctt gcctatggat
     4441 tggcttctcc cgcgttctct tcagaaactg ctacattaac gattaatggt aggatcagtc
     4501 ccccaacatg cagtatggct atggttaatg gtcaacctca acagcattgt ggtcagttaa
     4561 cgtataatgt tgatacccgt catcaggttt cttcgccagt taaaggtgta acgacggaag
     4621 tgattgctgc agatagcgat agcaaacgtc gaatcgtgtt aaatcgctac gattaatttg
     4681 ccaccctggt tttaatcgga tttagaa
//
LOCUS       DAZKHK010000239         4671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKHK010000239 DAZKHK010000000
VERSION     DAZKHK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.240"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            65..172
                     /locus_tag="ACHOS5_005004"
     CDS             65..172
                     /locus_tag="ACHOS5_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9779667.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNWLNKRK"
     gene            548..655
                     /locus_tag="ACHOS5_005005"
     CDS             548..655
                     /locus_tag="ACHOS5_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9779668.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(742..2421)
                     /gene="bcsG"
                     /locus_tag="ACHOS5_005006"
     CDS             complement(742..2421)
                     /gene="bcsG"
                     /locus_tag="ACHOS5_005006"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP9779669.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2418..2609)
                     /gene="bcsF"
                     /locus_tag="ACHOS5_005007"
     CDS             complement(2418..2609)
                     /gene="bcsF"
                     /locus_tag="ACHOS5_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP9779670.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2606..4177)
                     /gene="bcsE"
                     /locus_tag="ACHOS5_005008"
     CDS             complement(2606..4177)
                     /gene="bcsE"
                     /locus_tag="ACHOS5_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP9779671.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4450..4638
                     /gene="bcsR"
                     /locus_tag="ACHOS5_005009"
     CDS             4450..4638
                     /gene="bcsR"
                     /locus_tag="ACHOS5_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP9779672.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1222 a         1132 c         1201 g         1116 t
ORIGIN      
        1 aatcttgaac ctagaccact caagaatagc cgcgaaacgt tgtcattaca actcaggcgg
       61 ctatatgacg ctcgcagagc tgggcatggc cttctggcat gatttagcgg ctccggtcat
      121 tgctggcatt cttgccagtc tgatcgtgaa ctggctgaac aagcggaagt aacgtgtcag
      181 acgggcgtca ggctgccgta atggcaattt gcgcccggat caggccgcag ggataaaact
      241 ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc ggataatatt ctgcggaatg
      301 cgcagcacag agtgaagacg tgacggattc cggaacagaa aaacagtcgt agaatcagcg
      361 gcaagccgga agggttccgg tgaggcgcaa tgttgcgggg gcttaatccc tggtggcatt
      421 ggttgctgga gagagaaaac ccccgcatat tgcaggtatg cacctgacaa catcacgggg
      481 gctaatcttg aacccaaaca actcaagaat agccgcgaaa cgttgtcatt acaacacagg
      541 cggctatatg acgctcgcag aactgggcat ggccttctgg catgatttag cggctccggt
      601 cattgctggc attcttgcca gtatgatcgt gaactggctg aataagcgga agtaacgtgt
      661 catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg gatcaggccg caggggtgaa
      721 actctgcggc ctttttcgtt cttattgcgg gtaaggcacc cagtcgccgc cgttcaggcg
      781 aacgtacggt ttatcctggt attgaataac tactgcattt gagttctcgg agaccggtgc
      841 tgtttgtggc aacccactgg tgagtttttt ccagtcaaca ttgtcttcgg agaaaatctt
      901 gccatcaaga acgcgaacca ccagatcgga gatagccagg aagctgctcg gttggtcgat
      961 gacaatcggt gccccctgat gtggtgcctt catgccgaag aatttcaccc caacggggac
     1021 gtctgtgata gacgggctag ggatatcacg tagaccagat acctgcattc tgtcgccctt
     1081 cagcgcgccg ccgtgttccg gcaccacgac caccatcact ttacgacccg atttctccag
     1141 ttcagtaaag aaggcgtcca gttcatcaaa gaatttttgc gcccgcgctt tgtaatccgc
     1201 tgttttgctg acgcccggat aatggttgcc gtcatgcagt ggaagcgtgt tgtagaacgt
     1261 ggcactacgg ctgtttttat ctttttcggt aacgtccagc caacggttaa gcacggcggt
     1321 atcgtcataa accggcgaac catcaaagcc cagcaaaata accggcagat ttgtttgatc
     1381 catcaattcg ctctgcatgc cgccattttc gcgaacttcc ttcaaaaaac caccgaactg
     1441 gccgttatgt cccatcatca ggtgctgggt aaagcccagt ttcgacaggt tatcaaacag
     1501 atagcagtcg ttatttgccg gttgatacag attagtgtgc gaagtctgcc cgcagctggc
     1561 gcgcagtaaa cggatcgccg ccgggccact gtaggaggtg gcggagttaa agttcttgaa
     1621 ctcaatatcg aaatgcgacc acagtggatg cgacatcaat ccggcggctt caatatccga
     1681 ccaggaaagc gaacagatat taattaccag tagttcaaat ggctgagcat cagcgggcag
     1741 cgaagacggg aagatcgatt tacgtttcgc ttccgcgtta tagaaattat tcagccaggc
     1801 gttaaggttt gctgttgttg gcggtgcagt ttgtgcgggt atatcaccca ctaccggtgc
     1861 gccacccgtc gccgcaacgg ttgccgctgc gttaccaccc gtcgttgtta cagtggtcgt
     1921 cggttgaccg gctggccaca aggagaaact tggtcccgcc agggtaagta cgttcagcca
     1981 tagcagaatg gcaaccacaa aaacggtaat gcgaatccat tgtgacagga ataaccaggc
     2041 cacgaataaa acaaaaatgg ccccaatcat ctgccagtta ataaagcgtg tgacaagatc
     2101 gattaaataa tcggtactga accccgccac ctgcgaaccc tggctcatta tgctttccgg
     2161 gccaggcaac caggtgtcat gccagaacaa agcaaagccg atcggcaggg caatccagtg
     2221 gcgcaagcga tgcaggctgt agcgcggaat gggcatcagc agaaacgcgg caaacaccag
     2281 attgaggagc ggatggaagt taagatatcc cgcccacaac aggccgaact taaccagaaa
     2341 atagaagttc cagccggaaa ggccgcgcca gtattgccag agggaagaag gcatggcggt
     2401 attttgcgta aattgagtca ttttttggtt gccctggctt tttccgtgcg gcgtaacgtc
     2461 ccggccggtt taacataacg aggtttagca aagaacaaac gtaaggtgtc gcgaatgcgt
     2521 cgcaaagagt gccgcgccag atagcccagc gggaaaaata tcagtgcgca aacgacaata
     2581 atttcaatga tatcgctgat ggtcatcatg atgagcgctc cacagcatca tctaacagtc
     2641 gcattggttc tggtattcgt cgccagtggc gaccatcgtg ctcggcattg atgaccggtt
     2701 tagaactttg cgctaaaggc agcggcatgc cccattgttc tggggcaagc aggcgcatct
     2761 gcaccagctc ggcactgatt tgatcatctt caaaccagac cattcggttt gagaaaatgt
     2821 cgccagtagg caatgggaaa atatgattca acgcggtatc cagatcgtta atccgacaga
     2881 atgagagaaa cagcaccagc cgattaccgc caatggtcat gatatcgccg gtacggttag
     2941 ggcgacatag cgtcagggct tgctcaacgc ggatacccgg taccggacgt agggcaacca
     3001 gaacgccttt accgtgggca ggtaatagag ggttattcat catgttgttg acggcattac
     3061 agaacacatc ccacttctgg aaaccacgca gtttgagcgg ctgggtcatt gacagcaagg
     3121 tagtgatatc ttccggcaca tagcgactaa acttctgccc ttgcacgctt tcgatcatcg
     3181 tcagacagcg ggagagtggc gcattccacg ggataaccat atttgcaccg caggccaata
     3241 ataaacgttc atcggtggcg cgcaggctgg cggtattttc ccgtacgagg attttcatcg
     3301 cactaccgcg ctggcgacgc agagtatgaa tgctgcgggc cagtggctcg atttgtgcat
     3361 tttgctgtaa agaaaagacc accgtcgccg cctgagcggt acgggcttca ttgaacagga
     3421 cttcgttatt gttgaacagt tgccagtgtt ccgatagcgg cggcgcacct tccagtacag
     3481 caacattact cagaatgcgt ttttcgtcgc tgcgtggttg gatttccgcc tcctggtgtt
     3541 gaactaatgt ccagcaacca ttttgttgct gaacgctaag ctgctgacgg gcgctgaccc
     3601 ctttttcgtt gcaccagaaa gcaatatcca gcaaatgttg gtcgccctga aaacgcaaac
     3661 tggcaagacc aaaaagtgaa cggtattcct caagtaacaa tgaaaattgt ttatcgttat
     3721 tatttccgga attaattacc aaaagcgaac aatggtttag ccggctccat ttattcattt
     3781 tatccaacca tgagcgaagc cgctcggcag gaatgttttg ccatgcgtta tttgcgcaaa
     3841 caagaataaa aaggtaatta tggggatcaa tagaacactg caaatcacgg gccaaatagt
     3901 atagaccttt ttcatgattt agcattgaaa ataatgttat ttttcccggc ccttgagaat
     3961 catctaattg aaagattttc gctggatcgc tgtccatgct aatgaccgcg acgtgtgcgg
     4021 tttcagcctg ggatgcaatt gtttgattcg ccagactgat agcatcttca tggcgatcga
     4081 cgttaaacca ccagacgccg cctgctggca tatgtcgcag ctcatcccat aatgatgaga
     4141 taccgataga gaatacaggg tccacaatgt ccctcatgtc aacattttgt ctgattatca
     4201 gtttactagc gaaagctaag aaataaacct accattgaaa ttaaaactta tcagatttag
     4261 catgtgaatc aatttagcag gtcacaagac atgtgatttt agtcttttcg tttttaactt
     4321 tttctgttgc gtaacggaaa gtcaaaaagt gagcacattc ccgtctcgcc gtaaacaata
     4381 aaccgacgaa atgctcacag ttagaattgt ctcatgaacg gtacggttat ttcataggga
     4441 tcaagaaaaa tgaataacaa tgaaccagat actctgcctg atcccgcgat aggctatatc
     4501 ttccagaatg atattttggc gttaaagcag gcattttcac tgcctgatat tgattatgcc
     4561 gatatttccc aacgcgaaca gttggccgcg gcattaaaac gctggccgtt gctggcagag
     4621 tttgcgcaac aaaagtaggg gattggagaa tggccgtact gggattgcag g
//
LOCUS       DAZKHK010000240         4623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKHK010000240 DAZKHK010000000
VERSION     DAZKHK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.241"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(121..546)
                     /gene="nudI"
                     /locus_tag="ACHOS5_005010"
     CDS             complement(121..546)
                     /gene="nudI"
                     /locus_tag="ACHOS5_005010"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9779673.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQDIVWASPDTLKYMDLNEATRLTFSQKGIL"
     gene            856..1107
                     /locus_tag="ACHOS5_005011"
     CDS             856..1107
                     /locus_tag="ACHOS5_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9779674.1"
                     /translation="MPNIILSDISASVSELKKNPMATVGAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSSQPLHDVDLDSYS"
     gene            1104..1391
                     /locus_tag="ACHOS5_005012"
     CDS             1104..1391
                     /locus_tag="ACHOS5_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9779675.1"
                     /translation="MTYTIKFRDDALKEWLKLDKTIQQQFVKKLKKCSKNPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYYLASERMR"
     gene            1559..1963
                     /locus_tag="ACHOS5_005013"
     CDS             1559..1963
                     /locus_tag="ACHOS5_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFP9779676.1"
                     /translation="MTILVTAQAQLIVGEAMAIKSLAPDGMLAAVFEDDGNTGYFYAL
                     DESVEGNPVRDAVHIYNVEDISDAHIPSDVKIGWSEDCLKCVLLINGYPHGVFDFEGK
                     NGYCRSGFPPPVNHEWSVQGHAWDDYVDNFFR"
     gene            2273..3757
                     /locus_tag="ACHOS5_005014"
     CDS             2273..3757
                     /locus_tag="ACHOS5_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9779677.1"
                     /translation="MRKIIAHFKIVLTLLLPATVSAQSIEWQSCMTTPFSNWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDSSSVKRQQVKLALTKLPAKSRHKGSIIIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSIPAINCQQSDTGIQENITEKQRVLNKI
                     NSCIHNTGTEVIRHIGSNEAVYDIDRIRQALGDEQLTAVAYSYGTQIAALYAERFPSN
                     IRSIVLDGVVDIDDLGDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSVRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELILLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDALGVILCVDQGGEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSNLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVRHLITPMKMGNISCPGNAEIDIK"
     gene            complement(3978..4586)
                     /locus_tag="ACHOS5_005015"
     CDS             complement(3978..4586)
                     /locus_tag="ACHOS5_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP9779678.1"
                     /translation="MGKDPEEKKNPVIVVKKRRSFSPPSLAEKKDVIAPALTEQATES
                     VSSGISSPAVETHIPEAPARKKKKKRQRFPRPSHWTSEFTHDCLEKVKALFPQLRAEG
                     GGFLPLKTGITRDITTYLAAHPETALTLDEWNCAVSCITSRRIYLQRTSVAGTPRYNL
                     DGQPEGEVSECDAQNSRRWLAAKEQRWLSMKARQENISEKSK"
BASE COUNT         1301 a          987 c         1027 g         1308 t
ORIGIN      
        1 tgaaacgagc tcggtgcgat cacggcagca gacagataaa aatggcaaca aaccacccga
       61 aaaacagccg cgatcgcacc cgctaacagc cgttatcagt tgcatgatta gcattggagt
      121 ttaaagaata cctttctgag aaaaggtgag ccgggttgct tcatttaaat ccatatattt
      181 tagggtgtca ggagatgccc agactatatc ctgaaactcc tcattaaatg taactgtacg
      241 gtttgtactg atacagtcga atatgaggta aatcatgtaa atgtcctcgt gggtaccatc
      301 cgggtacgtt tttgtacgga tatcatcacg aaaggcccag ggttttacgt cagtgatatg
      361 cagttctttt cccagttctt ccttgatttc acgcctcaat gctgtttcca tagtctctcc
      421 gggttccatt cctccgcctg gcagtgccca ttgacccggg aatactccac gatcagaggc
      481 cattttacaa agtagaaact caccttcatt ttgtatcaca gggcacacta ttactctctg
      541 gcgcatttta tgtcctcaac atcatggctt tttggataac cttatcagaa aaatcatcat
      601 tttcatcact gacaggtcgt atgcctcaca agacgggaga taacttgaga atatttagtt
      661 cttctaccgg cttacgccgg ttttactcct gtgttatatg taaccactgt gtgccacctt
      721 ccatgccgct ggtgcagcat cagagtaaaa aatcagcatt aaatttattt gcaaatgtat
      781 gagtaataca gacttgttaa cgggtccttt cagatgtatc tttaagactt attaaacagt
      841 cttaaaaggt gcattatgcc taacatcatt ctgtctgaca tcagtgcaag tgtcagtgaa
      901 ctgaaaaaaa atccgatggc gacagtcggt gctggtgatg gttatccggt cgccattctg
      961 aatcgcaatc agccggcatt ctactgtgtt cctgctgaac tgtatgaaag gatgcttgat
     1021 gctctggatg accaggagct ggtgaaactg gttactgaac gtagcagtca acctctgcat
     1081 gatgttgacc tggacagtta ttcatgactt atacgataaa attcagggat gatgcgctta
     1141 aagaatggct gaaactggat aagaccattc aacaacagtt tgtgaaaaaa ctaaaaaaat
     1201 gcagcaaaaa tcctcatata ccgtctgcaa aactacgtgg gctaaaggac tgctacaaaa
     1261 ttaaacttcg tgcatcggga tttcgtctgg tttatcaggt gattgatgat atgttaatta
     1321 ttgcggttgt tgcggtaggt aaacgagaac gtagtaacgt ttattatctc gccagtgaga
     1381 gaatgagata gtaacataaa tagcctttcc cgcagataac tctcagaatt gttattctct
     1441 ctgtggcctc gcctgaggcg gaaaaagaaa agttttgttg agagaatgat tttacctgcc
     1501 gggatgagtc tggggttatt tatatcacca gacaaattga aacggaggga catgtttgat
     1561 gacaatactt gtgacggcac aggctcaact catagttggt gaggctatgg cgataaaaag
     1621 tttagctcct gatggaatgt tggccgctgt atttgaagac gacgggaata ccggatattt
     1681 ctatgcactt gatgagtccg ttgaaggtaa ccctgtcagg gatgctgtgc atatatataa
     1741 cgttgaagac atttcagatg ctcacatccc ctctgatgtg aagattggct ggtctgagga
     1801 ttgcctgaag tgtgttctgc tgataaacgg atatccacat ggggtattcg attttgaggg
     1861 gaagaacggc tactgtcgaa gtggattccc gccaccggtt aatcatgaat ggtctgttca
     1921 ggggcatgca tgggatgatt acgtcgataa tttcttccgg tgactgaacc gaaaaattac
     1981 ctgacggaca agtctggggc cgaatcgtga tgattcatca tggccgttta tagttcagca
     2041 gtcttgttct tttgctgtca gtgggagacc aggaaaataa ccgattgtta ccagacagaa
     2101 taaccatcat aagacgtcac aagacggtga cgcttacata gttccgggga gttaaacaat
     2161 ataccctgac tttgtaatca tttgaattat atccgtaacg tgtaacttcc tgccgtaaca
     2221 ccggatttaa gatattcaaa ctttatcttt attaccgata ttaagagaca caatgcgaaa
     2281 aataattgcc catttcaaga tagttttaac cttacttctg cccgcaacag tatctgccca
     2341 gtcgatagag tggcaatcct gtatgaccac tccattcagt aactggtttg gtgaggaaaa
     2401 accgtctcct gacttactat gtggttattt gtctgttcca ttaaaatata cagacacagg
     2461 cggagatagt tcttctgtaa aaagacagca ggtaaaactg gcgctgacaa aattgccagc
     2521 aaaaagcagg cataaaggga gtatcattat aataagtggt ggaccaggat tacctggcat
     2581 taatccttat attaactttg actggccggt aacaaatctt cgcgaatcat gggatattat
     2641 tggctttgat ccacggggag tcggccagtc cattcctgca ataaactgcc agcaatcaga
     2701 tacagggatt caggaaaaca taactgaaaa gcaacgggta ttaaataaaa ttaacagctg
     2761 tatccataat accggaactg aagttattcg ccatattggt tctaacgagg cggtatatga
     2821 tattgatcgt atcaggcaag ccctgggtga cgagcaactg acagctgtgg catattcgta
     2881 tggaacacaa attgcagcct tatatgcaga acgtttccca tccaacatca gatctatcgt
     2941 tcttgatggg gtcgtcgata ttgatgacct gggggacaac ttcacatggc aactcaaaca
     3001 agcacagagt tatcaggaaa cgttcgatcg ctttgcatcc tggtgtgcac gtacaaaaag
     3061 ttgcccactt tcttcagtca gagataaggc aataactcag ttccatgagc tgttatcaaa
     3121 attgcatcac aagcctttat tagacagcaa gggagaaaat atatcttcag atgaactcat
     3181 attattaaca acagaccttt tgttatggcg ttcatcatgg ccaacccttg caactgccat
     3241 acgtcagttc tctcagggga ttgtcagtaa tgaaattgaa actgcgctca gtgctcctat
     3301 agcctcagaa gagtcaagtg atgctctggg ggtaatcctt tgtgttgatc agggggggga
     3361 gcaattaacc ccagaagagc gaaaatcccg aaaagacgct cttgcgaatg ccttcccggc
     3421 tattaacttt gacaatggac gttccgattc acctgatttt tgtgaattat ggccaataca
     3481 tagcaacctg aataaaaccc ggctgaaaaa tacagttctg ccctctggtt tactgtttgt
     3541 agcacacaaa tacgatccaa caacaccatg gattaatgct cgtaagatgg cagagaaatt
     3601 ttccagcccg ttgctgacaa taaatggtga tgggcataca ttagctctta ccggagtcaa
     3661 tttatgtgta gataaggccg ttgttcgtca tcttatcacc cctatgaaaa tgggaaatat
     3721 atcctgtcca ggaaatgctg aaatagatat aaaatagttt aaaaattgcc cgccatttgc
     3781 tcctgataca ggacatattt tgcattggga gcaaaatgcg ttcagtgcga tcacggcacc
     3841 agaaacgtca aactggatac acgtaaaaaa tgtcgcgatc gcgcaaaatc aagccattac
     3901 tctcgggagt gcttaaataa tacgcacaat atacaacgct ctcttctcta cagtctgttt
     3961 cctgtaatat cctgcggtta ttttgatttc tcagaaatat tttcctgccg ggccttcata
     4021 ctcagccacc tctgctcttt cgccgccagc cagcgcctgg aattttgagc atcacattcc
     4081 gatacttccc cctctggttg accatccagg ttataacggg gagttcctgc aacagaggtt
     4141 ctctgcaggt agatccggcg actggtaatg caggatactg cacaattcca ttcgtccagg
     4201 gtcagcgctg tttccggatg tgctgccaga tatgtagtaa tgtcacgggt tatgcctgtt
     4261 ttcagaggca gaaaaccgcc cccttcggca cgaagttgag gaaacagcgc tttcaccttc
     4321 tccagacaat cgtgagtgaa ctcactggtc cagtgggatg gtcttggaaa gcgttgcctt
     4381 ttctttttct tcttcctggc tggcgcttcg gggatatgag tttcgacggc aggtgaactg
     4441 attccggagg agactgattc tgtcgcctgt tcagtgagtg caggcgcgat aacatctttt
     4501 ttttcggcta aggaaggcgg tgaaaaagag cgacgttttt tcaccacaat gacgggattt
     4561 ttcttttctt ccggatcttt tcccataaat acctctgctt cagaatttat tttttaataa
     4621 tat
//
LOCUS       DAZKHK010000241         4582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKHK010000241 DAZKHK010000000
VERSION     DAZKHK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.242"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..585)
                     /locus_tag="ACHOS5_005016"
     CDS             complement(<1..585)
                     /locus_tag="ACHOS5_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9779679.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGF"
     gene            complement(700..775)
                     /locus_tag="ACHOS5_005017"
     tRNA            complement(700..775)
                     /locus_tag="ACHOS5_005017"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(740..742),aa:Thr,seq:ggt)
     gene            complement(782..856)
                     /locus_tag="ACHOS5_005018"
     tRNA            complement(782..856)
                     /locus_tag="ACHOS5_005018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(821..823),aa:Gly,seq:tcc)
     gene            complement(973..1057)
                     /locus_tag="ACHOS5_005019"
     tRNA            complement(973..1057)
                     /locus_tag="ACHOS5_005019"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1021..1023),aa:Tyr,seq:gta)
     gene            complement(1066..1141)
                     /locus_tag="ACHOS5_005020"
     tRNA            complement(1066..1141)
                     /locus_tag="ACHOS5_005020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1106..1108),aa:Thr,seq:tgt)
     gene            1503..2453
                     /gene="coaA"
                     /locus_tag="ACHOS5_005021"
     CDS             1503..2453
                     /gene="coaA"
                     /locus_tag="ACHOS5_005021"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9779680.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2482..3447)
                     /gene="birA"
                     /locus_tag="ACHOS5_005022"
     CDS             complement(2482..3447)
                     /gene="birA"
                     /locus_tag="ACHOS5_005022"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP9779681.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3444..4472)
                     /gene="murB"
                     /locus_tag="ACHOS5_005023"
     CDS             complement(3444..4472)
                     /gene="murB"
                     /locus_tag="ACHOS5_005023"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9779682.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCIGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPSTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1175 a         1151 c         1040 g         1216 t
ORIGIN      
        1 gaagccagcc agttccagga ttttcgcttc ccactctgcg tcgccttcca gcgctttcag
       61 agcagaacca cgaacgatcg gtgtgtcgtc gcccgggaag tcgtactgag acagaagttc
      121 acgaacttcc atttcaacca gttccagcag ctcttcgtca tcaaccatgt cgcatttgtt
      181 caggaacacg atgatgtacg gaacgcctac ctgacgaccc agcaggatat gctcacgagt
      241 ctgcggcatc gggccgtcag tcgcagcaac taccaggatc gcgccatcca tctgagcagc
      301 accggtgatc atatttttaa catagtcggc gtgccccggg cagtctacgt gtgcgtagtg
      361 acgggtcggg gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc
      421 ttccggcgcg ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag
      481 tacggtagtg attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc
      541 gacgttaacg tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa
      601 gacacggata aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat
      661 gcaataaaca gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg
      721 aactgccgac ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc
      781 ttggagcggg cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc
      841 attatacgat gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc
      901 gagagaagct ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg
      961 cgtggccgaa attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt
     1021 tacagtctgc tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg
     1081 gaatcgaact ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg
     1141 catcaagtag cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa
     1201 tttgttttgt tggtcacatt ttgttcgaca cgatgaagaa acagccgggt taagagcaga
     1261 ttagcatact ttttcagcat cactctgctt aacaatgtgc gccagctaaa tcgcaattct
     1321 ttttatcaga tggaatatct gtcacattgc ttttcaacga tagcttcctg ggagagattt
     1381 tttcttatta ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa
     1441 ctgtgacgcg ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc
     1501 ttatgagtat aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt
     1561 gggcagctct gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca
     1621 aaggtattaa tgaagatctc tcgttagaag aagttgccga gatctattta cctctgtcac
     1681 gtttgctgaa cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc
     1741 ttggtaccaa cgggcaacgc attccttaca ttatcagtat tgctggtagt gtcgcggtgg
     1801 ggaaaagtac aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc
     1861 gtgttgaact gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg
     1921 gtctgatgaa gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg
     1981 tttccgatct caaatccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt
     2041 atgatgtgat cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag
     2101 ggttaaatgt cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt
     2161 ctgattttgt cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt
     2221 atatcaaccg ttttctgaaa ttccgcgaag gggcttttac agacccggat tcctattttc
     2281 ataactacgc gaaattaact aaagaagaag ctattaagac tgccatgaca ttgtggaaag
     2341 agatcaactg gctgaactta aagcaaaata ttctacctac tcgcgagcgt gccagtttga
     2401 tcctgacgaa aagtgctaat catgcagtag aagaggtcag actacgcaaa taattttcag
     2461 gggagctaat gctccccttt cttatttttc tgcactacgc agggatattt caccgcccat
     2521 ccagggtttt attattccat cctgctcaag taataaagca ccctgtttgt ctattccgcg
     2581 tgaaatgcca aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt
     2641 atccaacttt tcccagcgcg aaagataagg tgccaatcct tcttgctcga agagttccag
     2701 cgccgcgcgt aattcacgta tcagcatggc cgcaagagta ttacgatcga gattgatccc
     2761 ggcttcctgc agcgtgatcc acccttgatt aacgacactc tcttcaacac gacgcattgc
     2821 catgttgatc cctgcaccaa tgactatttg cgccgcatcg ccggttttgc cagtcagctc
     2881 cacaagaatg cctgccagct tgcgatcctg cagatagagg tcattaggcc atttaacacg
     2941 aactttatct gcacccagct tgcgtaatac ttccgccatc acgataccga taaccagact
     3001 taaaccgatc gccgccgccg ggccctgttc cagacgccag aacatcgaca aatataagtt
     3061 cgcgccaaaa ggcgaaaacc atttccgacc acgacgacca cgaccagcat gctggtattc
     3121 tgcaacacag gcatcgccag atttaagctc tccgatgcgg tccaggagat actgattcgt
     3181 ggagtcaata actggcagca cggctacact gccgtcatcc agctgactca atatcttttc
     3241 ggcattaagt aactggattg gctcgggcaa gctgtatcct ttacccggaa cagtaaatac
     3301 atcaacgccc cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc
     3361 cagcgtttca cccaactgct cgccagagtg aaattcgcca ttcgctaaca gggcgatcaa
     3421 tttcagtggc acggtgttat ccttcatgaa attgtctcta ctgcgctcac ttcacctgat
     3481 gcaccaataa agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt
     3541 cttacatgat gcgccagctg tacaacatct tcgctttttg catcgtgctc atttatgaga
     3601 accagcgcct gttgacggtg taccgcagcc ccaccaattt gcgtcccttt aagctggcac
     3661 tgatcaataa gccaacctgc agccagtttt actgaaccat ccacctgggg gtaatgtggg
     3721 gctgttggaa attgtgccaa caacgcgtta gcagtttcgg cagaaacaac aggatttttg
     3781 aagaaactac ccgcattacc gttcactttt ggatcaggga gtttggtggt gcgcatatga
     3841 cacaccgcat caaatacttg ctgtggcgtt accgttgaag gatccaggcg agttaagtca
     3901 ccatacgtca gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg
     3961 aagcgatcct ggtattcatg tttaaaaata ctgtctcgat agccaaaacg gcactctttg
     4021 gcagttaagc gtacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact
     4081 cgctgtaatt ctacgccata agcaccaata ttctggatag gtgatgagcc gatacaacca
     4141 ggaattaatg ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg
     4201 cgatgccagt tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt
     4261 tcgatacctt tgatccggtt gatgatcacc gtgccgcgat agtcctccag aaaaagtaca
     4321 ttacttcctt cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca
     4381 ttgagtaatt gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca
     4441 aatgtgttcc agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacgaggc
     4501 tagtttaccg tatctgtggg gggatggctt gtagatatga cgacaggaag agtttataga
     4561 aacgcaaaaa ggcccagtct tc
//
LOCUS       DAZKHK010000242         4522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKHK010000242 DAZKHK010000000
VERSION     DAZKHK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.243"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(268..549)
                     /locus_tag="ACHOS5_005024"
     CDS             complement(268..549)
                     /locus_tag="ACHOS5_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779683.1"
                     /translation="MKTNKKGTKWHIFYRENSGAEVLLEIPSFRECLSVSKELMTPSN
                     YMICIEKNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(607..999)
                     /locus_tag="ACHOS5_005025"
     CDS             complement(607..999)
                     /locus_tag="ACHOS5_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779684.1"
                     /translation="MELTVDLLKSLNNIRQNATIYVHAYHYTDDEKCAMVPRISLPSL
                     KLTPLQSEIIIRLLLNGVDYIRHDGYVQGYREKTVATFTIQGEGRHRFKADLSQNGES
                     DIASNVLNHWLHRIHKTDIIVLSVVLTE"
     gene            1616..1843
                     /locus_tag="ACHOS5_005026"
     CDS             1616..1843
                     /locus_tag="ACHOS5_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFP9779685.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            2221..2796
                     /gene="dolP"
                     /locus_tag="ACHOS5_005027"
     CDS             2221..2796
                     /gene="dolP"
                     /locus_tag="ACHOS5_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9779686.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRLSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADVASRVSGVKRVTTAFSCIR"
     gene            complement(3226..4503)
                     /locus_tag="ACHOS5_005028"
     CDS             complement(3226..4503)
                     /locus_tag="ACHOS5_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9779687.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAK
                     ANFAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRG
                     AGIEAYMPETKRWNPGDVVKSRMSDKQLNRLMEIDKIISAYNSQGTLSAEETKQLEEV
                     KKERADMFATYMATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQ
                     TVTEKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEE
                     HKLLISENSKQISTNTLGIRENTQRIRDEGQARIRGDRETLRAATGYTDMRVNGLERN
                     TNKRFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAG
                     FHFNTSENSAVKVNAAWDTENGAGVSAGMALGW"
BASE COUNT         1175 a          984 c          996 g         1367 t
ORIGIN      
        1 atcagcatga tgtctgtgtt ttgaatgtgt ggggtttttg gggcgagtaa tgtacgcgcg
       61 tattctctag aggtttaatt taaaaggcta cgtatgtagc tttatgtatt taagagtact
      121 gaactcagtc tccggcaata aaccagtttg gcgtccagct aacaaactct tgcggatgaa
      181 aggtccgggc acagcgatga cttacaagca ttctcccgca aaaaatgaac tatagcgcct
      241 tgcttaatgc attgtctgaa tccggagtta ctttttaata attctgttaa tggttattag
      301 ctcacctaat atttcgaagt tatcaggtgg gcagttaatc catttgttag agccagcgat
      361 gatttccctg tcccagcgtt ttattctttc gccatttttt tctatgcata tcatgtaatt
      421 tgatggggtc attaattctt tagatactga aagacattcc cgaaatgatg gtatttcaag
      481 caaaacttcc gcgcctgaat tttcacggta aaaaatgtgc cattttgtgc cttttttatt
      541 agttttcacg tgcgtatgct cataggataa atataaaacc ttgtaacatt caggaggtaa
      601 caggatttat tctgtaagaa caacagacag aacaatgata tctgttttat gtatacgatg
      661 tagccagtga tttagtacat tgctggctat atcactttcc ccattttgtg ataagtctgc
      721 tttgaaacga tggcgtccct ccccctgtat tgtgaaagtt gccacagttt tttctctata
      781 cccctgaaca tatccgtcgt gccttatata atcgactcca ttgagaagca gtcgaataat
      841 tatttctgat tgcaacggag ttaatttcag tgatggtaaa gatatccgtg gcaccatagc
      901 gcatttttca tcatcagtat agtggtatgc gtgaacatat atggtggcat tttgcctgat
      961 gttgttaagg cttttcagca ggtctacagt caactccatc gcggtatcct tcaggttgtt
     1021 atcattcgtt gatgatgcga tctgttgaag gcgttggcga caattaattc tttttctctt
     1081 ctgaaaaatt tacggaatac aaaaaagatc tgtttttaac tgattgttac tttctgaaga
     1141 tattctctgc tccatttgct ttccgggtaa gacgtatggc tggttatggt atggtcggac
     1201 tgcagccaca gggggatgac tgtaatacct ctgatcacca cagtcagtca tctgtaaatg
     1261 ttaaatacct cacagttcag tcagtggatt attgtcggtc ttacgggata tttgttaaaa
     1321 aaatactgca tggtgaatcc ccctgagcgg cggggcatat cagtatcgta ggtgtttggt
     1381 cacatccctt atctgtgcgg gttcaggtgc tgatactgaa ctcaccggga ggcacccggc
     1441 accatgctac ataacactcc attacgctac actcgcccct ctccggaggg gttttctctg
     1501 caggatgtga atatgagatg ctgcatcatt cactgcgaat attgatggtg agaatcttta
     1561 ataatattaa tgtttatatg tactgttaca gtgttgatga aaggagataa aaacaatgtt
     1621 cccggagtac agagaactga tttcagcatt gaaaggaaat cataaacgct ttgattcttt
     1681 atttgaaaag cataataaac ttgatcacga aataaaaaga attgaagatg aatttggttc
     1741 agcgtattcg acgaaagtgg tcaggctgaa gcttgaaaaa cttgagatca aaagagaaat
     1801 ccagaaaatc cttgaagatg agtcagaaaa gggcgaacgg taatcataaa cggtatattc
     1861 aaagacgtgt tttctctgtt ttatgaaaaa ggcaccgggg gctccacggt gccttttagc
     1921 ctgtgtataa cgaaaaggag atcctgttca gcgaaccaga tagttaaaaa aagaatcttt
     1981 acgacggtat caaagttttc gtgcaaaaaa catgatcaat attaaattcc agtacatcca
     2041 tatccgatat aaatctttcc gtctgacaga tacagatatg ccacgtaacg catccgctgt
     2101 ttccggctgt gccggaggat aataacagtc tgtagcttgt tttgtttcac tgaaggtaaa
     2161 ctttttgtac gataaattcc gggaatatga agtaaaaatc aaaacaattc cgggggagat
     2221 atgcgtttat tatattctgc tgttgtcctg atttcagcct tattgttaca gggatgcgtt
     2281 gctgcaactg tggtgggtac agccgctgtc gggacaaagg cagcaaccga cccacgcagt
     2341 gtcggaacgc aggtggatga tgaagtgctg gagctgaggc tcagcagtgc actttcaaaa
     2401 gatgaacaaa tcagaaaaga agcacgcatc agtgagattg catatcaggg acgcgtgctg
     2461 ctgatcggtc agtctccgga ccgtgaatta tctgcccgtg cccgtcagat agcactcggt
     2521 gtcgatggtg ttcaggaggt ctataatgaa atccggcagg ggacacccgt cagtccggtc
     2581 ataatggcag gtgatgcctg gcttacgaca aaaattcgtt ctcagttact cacaagtgcc
     2641 cgtgtcaaat catccagtgt caaggtggtg acagaaaaca gcgaagtctt tttactgggc
     2701 ctggttacag agcaggaagg gcgagcggca gcagatgttg ccagtagggt gagcggagtg
     2761 aagcgggtca cgacggcctt ctcctgtata cggtgaaggc agtatcagag ttgcctttag
     2821 tgtaaattta cgttaaaaat cagaagagca cgtttccggg ccgttcatgc caccacgtga
     2881 acattcgtcg cggaataccg gaggcgtgct cattataata accaatatac ataattcctc
     2941 tttgacaccc ccctgaattc gggggatttt tttgcccggg tatgttcact cacagataaa
     3001 atcccggtgc gctccggatg tcgctgctgg taaataatct gagcttttat ccatacaggt
     3061 caaagagcgt ggcgttgaca cgtgtacgtc tggctccggt gcaggagcca gattgtccgt
     3121 attaattaca tcctgagccg atgcgataaa ctgaatcgct tcctgaatgg tggtgcagtg
     3181 tcccctggat aacaatatct gcgaacggct tctggcccgg aagcattacc atcccagagc
     3241 catcccggct gaaacgccag ctccattttc agtgtcccag gctgcattca ctttaacagc
     3301 actgttttca cttgtattaa aatggaagcc ggcagcaaca gcaccttcac tccggtatcc
     3361 ggccatggcc ataccaaatg agaccttttt cccgggtacg gacggtatcc ccgtcattgc
     3421 catcgcacct gcaatacctg catctgcacg ttttctgttt ttgtctatat cctctttaag
     3481 ctgcgcaaaa cgtttattcg tattacgctc cagcccgttt acccgcatgt cggtataacc
     3541 ggtcgctgca cgcagtgttt ccctgtctcc ccggattcga gcctgtcctt catcccggat
     3601 ccgttgtgta ttctctctga taccaagagt gttggtactg atttgtttgc tgttctccga
     3661 aataagcagc ttatgttctt ctgctgtctg tgtattggct gtcactcttt catccagaat
     3721 ttttgtcgtt ttattaatgt tactgatttc ctctttatta ctgttaacca cctgcagatt
     3781 tttagttatc gcactggtgt tgtttgttat gcgtttatca agcgctgcat ctgccttttc
     3841 tgtcacagtc tgcaaatccc tgagctgtgc aacgttaaca gcatccgtat cgacagaacc
     3901 tgctgcaacg ctagttatct gtcttgtaac agcatgtcgt cctgttgtcg ttgcaccaac
     3961 acctacagat acagcatcgt gcgttgccat atatgtcgca aacatatcag cccgctcttt
     4021 tttcacttcc tccagttgtt tagtctcttc agcagacagc gtcccttgtg aattataagc
     4081 ggatattatc ttatcgattt ccataagcct gttaagctgc ttgtcagaca tcctggattt
     4141 tactacatcc cctggattcc agcgctttgt ctctggcata tatgcttcaa taccagcgcc
     4201 acgatcagca acacttcctg cgcccagagc gacagagaat ggtttcaatg ttgttgcgtt
     4261 tgctcctaac gcggtagagt tattttcaat agctcttgct gatgcaccta aagaaatact
     4321 tttttctgca tttgcctgtg aatatcgacc aacagcaaca gcgaaattag cttttgccgc
     4381 actgttatca ccaagttcca cgatcatttt atcaggaata ccatttgcag ataagacagg
     4441 taaagaagct gagcagtaga gaaataatga tttgcatata taactgacac tgattttacg
     4501 catttgaatt caccatctct tt
//
LOCUS       DAZKHK010000243         4507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKHK010000243 DAZKHK010000000
VERSION     DAZKHK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.244"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1318
                     /locus_tag="ACHOS5_005029"
     CDS             <1..1318
                     /locus_tag="ACHOS5_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9779688.1"
                     /translation="RIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPK
                     TRSAGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAAT
                     KRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAA
                     ISAKARGLTDRQAQRESEAQRLRDVYGDNPQALAQVTGALKQTWADEDMLRGDWLAGL
                     KSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWRGFTRSVLSMMTEI
                     LLKQAMVGIVGRIGSAIGGAFGGGASASTGTAIQAAAANFHFATGGFTGTGGKYEPAG
                     IVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHV
                     VIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
     gene            1315..1644
                     /locus_tag="ACHOS5_005030"
     CDS             1315..1644
                     /locus_tag="ACHOS5_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779689.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            1644..2342
                     /locus_tag="ACHOS5_005031"
     CDS             1644..2342
                     /locus_tag="ACHOS5_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9779690.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KREPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLSINKLSQ"
     gene            2353..3096
                     /locus_tag="ACHOS5_005032"
     CDS             2353..3096
                     /locus_tag="ACHOS5_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9779691.1"
                     /translation="MTQTESAILAHARRCAPVESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSRAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVVASACM"
     gene            3093..3674
                     /locus_tag="ACHOS5_005033"
     CDS             3093..3674
                     /locus_tag="ACHOS5_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9779692.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGATMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            3915..>4507
                     /locus_tag="ACHOS5_005034"
     CDS             3915..>4507
                     /locus_tag="ACHOS5_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779693.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERNGHWVTEKDVTINGKTT
                     SQYLTSVILNNLPERPFNIRMVRETADSTTDQLQNRTL"
BASE COUNT          995 a         1084 c         1548 g          880 t
ORIGIN      
        1 gcgtatacgc cagggggaaa tggggtacat caaccgttcg cgtgcaacag gcgtcagcaa
       61 aggtccgggg cagcaggaag ccgtcagccg tctggctgaa gagctgacag gtaaaaagca
      121 tacatcaccg aaaacgcgct ctgccgggga gagggaagag gagcaggcaa gagaggctct
      181 gcttgccctt gaagctgagc tcaggacgct ggaaaagcac agcggtgcga atgagaaaat
      241 cagccggcag cgccgtgatt tatggaaggc ggaaagtcag tatgcggtcc tgaaagaggc
      301 tgccacgaaa cgacagttat ccgggcagga aaaatccctg ctggcccatg agaaagagac
      361 actggagtac aaacgccagc tggctgagct gggcgacaag gttgaatacc agaaacggct
      421 gaatgagctg gcacagcagg cggtgcggtt tgaagagcag cagagcgcga agcaggccgc
      481 catcagcgca aaagcccgtg gtctcactga ccgtcaggcg cagcgggagt ctgaagcgca
      541 gcgtcttcgt gacgtgtacg gcgataatcc gcaggcgctg gcacaggtca ccggggcact
      601 gaaacagaca tgggcagatg aagacatgct gcgcggtgac tggctggccg ggctgaagtc
      661 cggctggggg gagtgggcgg aaagtgcgac ggacagtttt tcgcaggtta aaagtgtggc
      721 cacgcagacc tttgacggta ttgcacagaa tatggcggcg atgctgaccg gcagcgaaca
      781 gaactggcgg ggattcaccc gttcggtgct gtccatgatg acagaaatcc tgcttaaaca
      841 ggccatggtg ggcattgtcg ggcgtatcgg cagcgccatt ggtggtgctt tcggtggtgg
      901 ggcgtctgcc tccacgggga cggccattca ggctgcggcg gcgaactttc attttgcgac
      961 cggcggattt acgggcacgg gcggcaaata tgagcctgcg gggatagttc accgcgggga
     1021 gtttgtcttc acgaaggagg caaccagccg gataggcgtg gggaatcttt accgtctgat
     1081 gcgcggctat gcggaagggg gttatgtggg tggtgccgga agtccggcgc agatgcggcg
     1141 ggcggaaggt attaatttta atcagaacaa tcacgtggtg attcagaacg acggtatcaa
     1201 cggacaggca gggccgcagc tgatgaaggc ggtgtatgac atggcccgca agggggcgca
     1261 ggatgagctc cggctgcagt tgcgtgatgg cggtatgtta tcagggagcg ggcgatgaaa
     1321 acctttcgct ggaaagtgaa gccggatatg gaggtgaact cgcagccatc ggtgcgtgaa
     1381 gtgcgttttg gtgacgggta ctcacagcgt atggcggcag ggctgaatgc tgacctgaaa
     1441 acataccggg tgatgctttc cgtgacccgg gaggaggccc ggcatctgga agcgttcctg
     1501 gcagagcacg ggggctggaa ggcatttttg tggaagccac cctatgcata ccggcagata
     1561 aaggtgacct gtgccgggtg gtctgcgcgg gtcgggatgt tgcgcgttga gttcagcgcg
     1621 gagtttaagc aggtggtgaa ctgatgcagg atattcacga agcaagcctg aacgagtcgg
     1681 ttaagtcaga gcagtcaccg cgggtggtgc tctgggaaat cgacctgacg gtgcagggcg
     1741 gtgagcggta ttttttctgc aatgagctga atgaaaaaag ggagccggtg acctggcagg
     1801 ggcgtgaata tcaggcgtac ccgattgagg gcagcggctt tgagatgaac ggaaagggca
     1861 gcagtgcccg cccgtcgctg acggtgtcca atctgttcgg tcttgtcacc gggatggcgg
     1921 aggatttgca gagcctggtg ggtgccacgg tggtccgtcg ccgggtgtat gcgcgttttc
     1981 tggatgcggt gaattttgtg gcaggcaatc ctgaggcaga cccggagcag gagctgacgg
     2041 accggtgggt ggtggagcag atgtcatctc tgacggccat gacggcctcg tttgtgctgg
     2101 cgacaccgac ggagacggac ggggcgctgt ttcccggtcg catcatgctg gcgaacacct
     2161 gtatgtggga ttaccggggc gatgaatgcg ggtataacgg tccggcagtg gcggatgagt
     2221 tcgacaaccc caccacggat atccggaagg acagatgcag taaatgcatg cgcgggtgtg
     2281 agatgcgcgg catggcggtt aatttcggcg gtttcctttc cattaataaa ctttcgcagt
     2341 aaatcccgtt ttatgacaca gactgaatca gcgattctgg cgcatgcccg gcggtgtgcg
     2401 ccagtggagt cgtgcggctt cgtgataagc accccggagg gcgaacggta ccagccctgc
     2461 gtgaatatct ccgcagagcc ggaggcgtat tttcgtattg cgccggaaga ctggctgcag
     2521 gcacagatgc agggggagat tgtggcgctg gtccacagtc atcccggtgg tctgccctgg
     2581 ctgagcgagg cggaccggcg gctgcagata aagagtgccc tgccctggtg gctggtctgc
     2641 cggggtgaaa ttcaccggtt ccgctgtgtg ccgcacctga ccggacggcg ctttgaacac
     2701 ggtgtgacgg actgttacac cctgttccgg gatgcatacc atctggcggg gattgagatg
     2761 ccggattttc accgcgagga tgactggtgg cgcaacggtc agaacctgta cctggacaac
     2821 ctggcggaaa acggctttta ccgggtgtct ccgtcccgtg cacaggcagg cgatattctg
     2881 ctgtgctgct ttggcgcatc ggtgccgaat catgccgcca tttactgtgg caacggtgaa
     2941 ctgcttcacc atatacctga acaactgagt aaacgggaga ggtattcaga gaaatggcaa
     3001 cgacgaacgc attctgtctg gcgtcaccgc cactggtccg catctgcctt cacggggatt
     3061 tacaacgatt tggtcgtcgc atcagcctgt atgtgaacac ggcagcggag gccatccgtg
     3121 ccctgtcgct gcaggtgccg ggattccgct gtcagatgaa cgaaggctgg tatcagatac
     3181 gtattgccgg tgaggatacc gcgccggagg cggtgtatgc ccgtcttcat gagccactga
     3241 gcgggagggc cgtgattcat attgtaccga ggctggcagg ggccggggga aatggtgttt
     3301 ttcaggtggt gctgggggct gcagccatcg tgggttcttt cttcaccgcc ggtgcaacga
     3361 tggcgttgtg gggcgcagcc ctgagtgccg gagggctgac tgccaccacg atgctgttct
     3421 cactgggtgc cagcatgata ctgggtggtg tggctcagat gctggccccg aaggcaaaga
     3481 cgccggagta caaaagtacg gataacggta aacagaacac gtatttttcg tcactggaca
     3541 acatgattgc ccagggtaac ccgatgccgg tgccttacgg tgaaatgctg gttggttcac
     3601 gacggatatc ccaggacatc agcacccgtg atgagggcgg tgacgggaaa gtggtggtta
     3661 tcgggcgggg atgaaaataa aaaaatcccg cagagttagc ggagctgcgg gagagaacga
     3721 tgaagattaa cgttatggag ttatttttca ggcatcaaaa aagtaacgca gcgtcattat
     3781 tgcggctaca ggcaattgcc ggaaatgtga agagtttcag aaattttatt ccgtcatgac
     3841 acaggcaccc tccggggtgc ctgttgtttt ctggcataaa cagattcaga catcagacag
     3901 gagaggggga cagagtgggt aaagggggcg gcaaggggca cacaccgcgt gaggcgaagg
     3961 acaatctcaa atccacgcag atgatgagcg tgattgatgc cattggtgag ggaccggtgg
     4021 aaggtccggt gaagggactg cagagtattc tggtgaacaa aaccccgctg acggacacgg
     4081 acggtaatcc cgtgatacac ggtgtgaccg ccgtctggcg tgccggggag caggagcaga
     4141 caccgccgga aggctttgag tcctccgggg cagaaaccgc actgggcgtg gaggtgacga
     4201 aggcaaagcc ggtgacgcgc accatcacgt cagcgaacat tgaccgtctg cgggtcacct
     4261 tcggggtgca gtcactggtg gagaccacct caaagggtga ccgtaatccc tcttctgtcc
     4321 gcctgctgat tcagcttgag cgtaacgggc actgggtgac ggagaaggat gtcaccatta
     4381 acggcaagac cacctcgcag tacctgacgt cggtgattct gaataatctc cctgagcgcc
     4441 cctttaacat ccggatggtc agggagacgg cggacagcac cacggaccag ctgcagaaca
     4501 gaacgct
//
LOCUS       DAZKHK010000244         4436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKHK010000244 DAZKHK010000000
VERSION     DAZKHK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.246"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..872)
                     /locus_tag="ACHOS5_005035"
     CDS             complement(<1..872)
                     /locus_tag="ACHOS5_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9779694.1"
                     /translation="MKRAPNLKYQPRDKMTEVIIFAGSDAWSHAKEWNEWAGKHIAAD
                     DTPPVILGTEQLENLDDMQIIDEGRHYVRVYRAGKIAEKSLTKVATLLAIAGVKEARC
                     YRSFVDREPEDWTPRLVGLKAEAEHGESLVIELPVKKAERKNDERASSLALNQMGASQ
                     RGEVLLAHYGGELAINADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAI
                     KSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAE
                     GETLATHAPNFWKW"
     gene            complement(869..1156)
                     /locus_tag="ACHOS5_005036"
     CDS             complement(869..1156)
                     /locus_tag="ACHOS5_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9779695.1"
                     /translation="MKNKFSGFIASGQTHSKISLGDIFKDSYGYRVKIISVDDRRVSY
                     LRDGYDFECVMPRQQFERDFILVKNCKTDNQRRAAGYIRKIRAMLVAGGNK"
     gene            complement(1160..1384)
                     /locus_tag="ACHOS5_005037"
     CDS             complement(1160..1384)
                     /locus_tag="ACHOS5_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779696.1"
                     /translation="MNDLYFKVLTHAENALVCGKNMREILSTWLDGTTNAEHDERDAN
                     LAGALITLLDPVIKELDEAIKIHDQSYTGE"
     gene            complement(1377..1742)
                     /locus_tag="ACHOS5_005038"
     CDS             complement(1377..1742)
                     /locus_tag="ACHOS5_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9779697.1"
                     /translation="MAGTQHTQTHPKFIYIFLALHRDRIADGATTVHVAADTLVDARK
                     MVKEMGYTAAFWKGREENTLFIQKCENNFIWRFIALSTAQPRVITIEATSEQEARQQS
                     PTGCVMIFAARIRQEVCHE"
     gene            1879..2073
                     /locus_tag="ACHOS5_005039"
     CDS             1879..2073
                     /locus_tag="ACHOS5_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9779698.1"
                     /translation="MSTTIQRDKQPKGAGKAPAFQIRINPELKEQMAESAAQEGMSLA
                     NWIKELVRAELRRKGIEPKG"
     gene            2106..2789
                     /locus_tag="ACHOS5_005040"
     CDS             2106..2789
                     /locus_tag="ACHOS5_005040"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFP9779699.1"
                     /translation="MTPTPDDAVFTASNIINFFVALGTCTAVLVALIAKKQSSFESTF
                     SLMLSQHNNALNDLRKKSNYSELINKMMNGLVSLKDTNTLMHNHDAVFGSYFRILYHL
                     LKFIDENAGYHPFDLDRKKTYTSLVRAHLDNEITYLLAINCAHANSENQYHFYKTLIE
                     RYAMLEHLILDKSTLLDYTPPDLNEYQKDLRVATIVVTDDIKTIFNEIATVYYKSAFG
                     KNPDLKNFL"
     gene            complement(2816..3037)
                     /locus_tag="ACHOS5_005041"
     CDS             complement(2816..3037)
                     /locus_tag="ACHOS5_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9779700.1"
                     /translation="MKKMAIVDKKGLEYIPNIDRMIREKECRELTTLANSTRWKLEKE
                     GKFPKRIKIGSTAVAYRLSEIQAWIRGEW"
     gene            complement(3039..4274)
                     /locus_tag="ACHOS5_005042"
     CDS             complement(3039..4274)
                     /locus_tag="ACHOS5_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HFP9779701.1"
                     /translation="MAGENKLSDKALKGYLGKPREKQITIADGKGLSIRVSTKGAVSF
                     VFFYRLAGGRAAPVWLTLGKYPDMSLKQAREKRDECRGWLADKRDPRIQIKIQAEERL
                     KPVTVEDALNYWYENYCKVRRKTHAVTLGRFRKHIFPYIGHLPVNDTHLYEWLDCFDR
                     IKRNAPVMAAYVFSDTKLALRFCRVRQYATCDALKDLRMSDVGQIAGKRDRVLDEAEL
                     GQLWKAIFVEPDLKLMSEYTRKMFVLCTVFGCRMSEARLSEWSEWDLESWVWTVPKDH
                     SKTGVEIVRPVPEILRQWVTDVHEETKHTGYVLGSLRIRESVSKIGGKIGKRLGHEKQ
                     WSLHDLRRTLSTHLSDLGVEFYVVEQLLGHALPGVAGVYNRSKFMAKKLDALELWTTY
                     LNSIAAADSKVTILKQKAG"
BASE COUNT         1236 a         1079 c          839 g         1282 t
ORIGIN      
        1 aaccacttcc agaaattcgg cgcatgtgtt gccagcgttt ccccctctgc tggtgggctg
       61 aaaggtaatt cactggcaat taacaaccag tcgtttttgt tatgctcccg aaaattacca
      121 gttctggtat caaatacccc gttactgaat ccaatcaggt tacgggctgt attccccatt
      181 acaggcaaac ttaacttcat ggtatctacc gccgatttaa tggcgttctg cgaatagctg
      241 atctccgcat caatgaaaat ctgcgccata gcacgctgta actctttatc ctgaaccggc
      301 tcccatacaa cgccgttgta atgatgaacg gtgtcagagt cggcattgat tgccagttcg
      361 ccgccgtaat gtgcaaggag aacttcaccg cgctggctgg cccccatctg attcaacgcc
      421 aaagatgaag cacgctcgtc atttttgcgc tctgccttct tcactggcag ttcaatcacc
      481 agactttccc catgctccgc ttcagctttt aggccgacaa ggcgcggagt ccagtcttca
      541 ggctctcgat caacaaagct acggtaacaa cgtgcttcct ttacgcctgc aatagcaagt
      601 aatgtcgcaa ccttcgtcag acttttctct gcaatctttc cggcacgata aacacgcaca
      661 taatgacggc cttcatcgat aatttgcata tcatccaggt tttccagttg ttccgtaccc
      721 agaatgactg gtggtgtatc atctgctgca atatgcttac ctgcccattc attccattct
      781 tttgcatggc tccaggcatc actgccagca aaaatgatga cttccgtcat tttgtcgcgc
      841 ggttggtatt ttaagttcgg agcacgtttc atttgttacc tccggcaact aacattgccc
      901 gaattttacg gatatagcct gcggcacgcc tctgattatc tgtcttgcaa ttttttacca
      961 gaatgaaatc tctttcgaac tgctgacgcg gcataacaca ttcaaaatca taaccatcac
     1021 gcaaataaga gacacgacga tcatcaaccg aaataatctt tacccgatag ccatagctat
     1081 ctttgaaaat atccccaagg ctgatttttg aatgagtttg accgctggca ataaagccag
     1141 aaaatttatt tttcattttt tattctccgg tatagctctg gtcgtgtatt tttatagctt
     1201 catccagctc tttgatgaca ggatcaagta acgtaattaa cgctccagct aaattagcat
     1261 cccgttcatc gtgttccgca tttgttgtcc catcaagcca ggttgataag atttctcgca
     1321 tatttttgcc acaaacgagc gcgttttcag catgtgtcag cactttaaaa taaagatcat
     1381 tcatggcaca cctcctgacg aatacgggcg gcgaatatca tcacgcagcc agttggggat
     1441 tgctggcggg cttcctgttc gctggtggcc tcgatggtaa tcacgcgcgg ttgtgccgta
     1501 ctcagggcga taaaacgcca gatgaaattg ttttcacatt tctgaataaa cagcgtgttt
     1561 tcttcccgcc ctttccagaa agccgcagta tagcccatct ccttaaccat cttgcgcgca
     1621 tcaaccagcg tgtcagcggc tacatgtacc gtagttgctc catctgctat gcgatcgcga
     1681 tgtagcgcca gaaagatgta tataaattta gggtgagttt gggtatgctg tgttccagcc
     1741 atagtcgtta ctccagttaa cggtttgtgg ttagacgccc taggtgtgtt gcgagcaccc
     1801 tagggcgttg tttataggta taacatcatg ttaatgtgtg tacctattca acactgatgc
     1861 taagcaatag gtacacacat gtcaactaca attcaaagag ataagcagcc aaaaggagcc
     1921 ggcaaagccc ctgcgtttca gatccgaatc aatcctgaac tcaaagaaca gatggctgaa
     1981 tcagcagctc aggaaggaat gagcttggcc aattggatta aagaattagt tcgtgctgag
     2041 cttcgccgta aaggtataga acctaaaggc taattttttt aatttttatt gaataggata
     2101 ttcaaatgac tccaactcct gacgatgctg tattcactgc atcaaacatt ataaatttct
     2161 tcgtggcact cgggacttgc acagcagtgt tagttgcatt aattgctaaa aagcaatcat
     2221 cttttgaatc aacattttct ctcatgttat ctcaacataa caatgctcta aatgatctta
     2281 gaaaaaaatc aaattactca gagttaatta ataaaatgat gaatggattg gtttctttaa
     2341 aagacactaa tacactgatg cacaatcatg atgctgtttt tggaagctac ttcagaatat
     2401 tatatcatct attgaagttc attgatgaaa atgctggata ccatcccttt gatctagatc
     2461 gtaaaaaaac ctatacaagc cttgtccgtg cacatctaga taatgaaata acgtatttac
     2521 ttgcaataaa ctgtgcacat gctaacagtg aaaatcaata tcatttttat aaaacattga
     2581 ttgagcgcta tgccatgtta gaacatttaa tcttggataa atcaacgcta ttggactata
     2641 cccctccaga tttaaatgaa tatcaaaaag atttgcgcgt agcaacaata gtggttaccg
     2701 atgatataaa aactattttt aacgagattg caacagtata ttataagagc gcatttggaa
     2761 aaaaccctga tttaaaaaat ttcctgtaag atatagggag gaatacctcc ctaacttacc
     2821 attcacctct tatccaagct tgaatctcag agagtctata agccaccgcc gtagatccga
     2881 ttttgattcg cttggggaat ttcccctcct tttcaagctt ccagcgtgtg ctgttcgcaa
     2941 gagtggttag ctcccgacat tctttctcac ggatcattcg gtcaatgtta ggaatgtact
     3001 ccagcccctt tttatcaaca attgccattt ttttcatgct aaccagcctt ttgtttgagg
     3061 attgtcactt ttgaatcagc agctgcgatg ctattgagat atgtagtcca gagttccaga
     3121 gcatccagtt ttttagccat aaacttactc cggttgtaaa cacctgccac gccaggtagc
     3181 gcatggccta acagttgttc tactacataa aattcaacac cgagatcact tagatgagta
     3241 gatagcgttc ttctaaggtc gtgtagtgac cattgttttt catggcccaa acgtttaccg
     3301 attttccccc caatcttgct tacgctttct ctaattcgca gacttcccag cacataacca
     3361 gtatgttttg tctcttcgtg aacatccgtt acccactgcc gtagaatttc aggtactggc
     3421 ctgacaattt caacaccagt ttttgagtga tctttcggta cagtccaaac ccaactttct
     3481 agatcccatt cgctccattc tgataatcgg gcttcactca ttcgacatcc aaatactgta
     3541 caaagcacaa acatttttcg cgtgtattca gacattagtt ttaaatcagg ctcgacaaaa
     3601 attgccttcc agagctggcc cagttcggct tcatccagaa cccgatcccg cttacctgca
     3661 atctgcccca catcactcat gcgcaaatcc tttaaagcat cacacgtcgc gtactggcgt
     3721 acccgacaaa aacgaagagc taatttagtg tcagaaaaaa catacgccgc cataactggt
     3781 gcattacgtt taattcggtc aaaacagtcc agccattcat ataggtgagt gtcatttacg
     3841 ggcaaatgac cgatataggg aaagatatgc tttcgaaatc tgccaagcgt tacagcatga
     3901 gttttacgac gcaccttaca gtaattttca taccagtaat ttagtgcatc ctccactgtg
     3961 accggcttta agcgttcttc agcctgaatc ttaatctgaa tacgcggatc acgtttgtca
     4021 gccaaccaac cacggcactc gtcgcgcttt tcccttgcct gtttgagtga catatcagga
     4081 tatttaccca acgttagcca gaccggagca gcccggccac ctgctaacct gtagaagaaa
     4141 acaaagctca cagccccctt ggtactcaca cgaatagaaa gcccctttcc atcagcaatg
     4201 gtgatctgct tttctctagg tttccccaga tatcctttaa gtgctttgtc gctcagtttg
     4261 ttctcgccag ccatttttag ccccaaaaag caatacaagc tgcaatacag agatgattgc
     4321 aacacacaga taacgaggaa aatccagtga aagcgccaga taaacttact ctttattatc
     4381 aaaagattaa gtgtaaaaac cagcaactac acgaaagcct cagaaagcca tgctaa
//
LOCUS       DAZKHK010000245         4375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKHK010000245 DAZKHK010000000
VERSION     DAZKHK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.247"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..617
                     /gene="eco"
                     /locus_tag="ACHOS5_005043"
     CDS             108..617
                     /gene="eco"
                     /locus_tag="ACHOS5_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP9779702.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(767..2413)
                     /gene="mqo"
                     /locus_tag="ACHOS5_005044"
     CDS             complement(767..2413)
                     /gene="mqo"
                     /locus_tag="ACHOS5_005044"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP9779703.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKHDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(2631..4274)
                     /gene="yojI"
                     /locus_tag="ACHOS5_005045"
     CDS             complement(2631..4274)
                     /gene="yojI"
                     /locus_tag="ACHOS5_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP9779704.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQNGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLIEKWLVQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELIGEERDAASRDAVARTA"
BASE COUNT         1096 a         1180 c         1057 g         1042 t
ORIGIN      
        1 aagagcaggt aaaattatct gaatgttctt taagttattc ataaagcaaa ttaataaatt
       61 tgatgaatat gttaaccttc agcgacatca tcggtgaaaa cctataaatg aagaaggaaa
      121 gcaaaaaaat gaagaccatt ctacctgcag tattgtttgc cgctttcgcc accacttccg
      181 cctgggcggc agaaagcgtc cagccactgg aaaaaatcgc accttatcca caagctgaaa
      241 aaggcatgaa gcgtcaggtg attcagttaa ctccgcaaga agatgaatct accctgaaag
      301 tagaactgtt aatcggtcag acgctggaag tcgactgcaa tttgcatcgt ctcggcggga
      361 agctggaaag caaaacgctg gaaggctggg gctatgatta ttatgtcttt gataaagtta
      421 gctcgccggt ttccacgatg atggcttgcc cggatggcaa gaaagagaag aaatttgtca
      481 ccgcgtatct gggcgatgct ggaatgctgc gttacaacag caagctgccg atcgtggtgt
      541 atacgccaga caatgtggat gtgaagtacc gtgtctggaa ggcggaagag aaaattgaca
      601 acgcggttgt ccgctaaact gctgtgaagt gctgcaaccc gtaggccgaa taaggcggtt
      661 acgccgcatc cgacatctaa cgcccgagcc ggttgcctga tgcgacgctg aagcgtctta
      721 tcagcatacg ccacatccgg caaaccatgc cggatgtggc acatcattac aacgcaatat
      781 ccgccacttc tttttgaacg ggttgcggtt tcaactgcgg tttcggcgta ctatctgctg
      841 cctgcggctt gtcgtatttc agccccagca cttcgctggt gtactgcaac tcgcgttctg
      901 ttgccgctac atcaccgttc agcttgcgtc cataagacgg aacgatcgct ttcaacgtag
      961 cctgccattg cgggctggaa acgcgatcgc caaatacttt ttccagcaga ttcaacataa
     1021 tcggcgcggc ggttgacgct cctggcgatg cccccaagag cgcggcaatg gtcccttgct
     1081 ggtcactgac gacttcagta cccagacgca gtacgccacc tttatcggca tcatgcttga
     1141 taatctgcac gcgctgcccc gcttgccaca aacgccagtc ctcttttttc gcctgcggat
     1201 agtactcttt caacgcttca aaacgatcct cttcactcaa catcacctga ctcaccagat
     1261 atttcaccag atcgaaatta tccagcccaa cgtgcatcat cggcatcacg ttagaggtgg
     1321 tggtggaact catcagatcc cacaatgaac cgtttttgag gaatttggtt gagaaggtgg
     1381 caaatggccc aaacagcact acgcgtttac cgtcaagaac acgggtatcg atatgcggaa
     1441 ccgacatcgg tggtgcgcca acggatgctt taccgtaaac cttcgccaga tggtgattaa
     1501 ccacgtccgg gttttccgaa acaaggaact gtccgcccac cgggaaaccg gcgtagtctt
     1561 tcgcctccgg aatccccgat tcctgtaaca gtttcagcgc cgcaccgccc gcgccgataa
     1621 agacaaattt ggcgcggatg ttctgtgcag tgccgttttt cagatcggca acggtgacgg
     1681 tccagctgtt atcgtcatta cgctttaggg cgcggacttc gctgctgagt tgcagcgaga
     1741 agttagattt cttctgtaag gaggcgatca actggcgggt gatctcgcca tagttcacat
     1801 cagtgccaat ttctgtgcgt gttgccgcta ctttctgttg cggatcgcgc ccttccatca
     1861 ctaacggtgc ccactctttg atctgcgcgt gatcttcaga gtaacgcata ccgcgaaaca
     1921 gcgagctttg ttgcaacgcg gcgtaacggg cgcgcaggaa attaacgtta tcctcgcccc
     1981 agacaaagct catatgcgga acggtattga taaatgaacg cggagtacgc agcacaccgc
     2041 gctcgacctg gtgcgcccag aactggcggg aaatctgaaa tgcttcgtta atggcgactg
     2101 ctttttcaat actgatgctg ccatcggcgt tttgcggagt gtaattgagt tccatcagtg
     2161 cagaatgccc ggttccggcg ttgttccagc cgttcgaact ctcctgcgcg acaccctcca
     2221 ggcgctccac catggtcatc gaccattcag gctccagctc gcgtaaatag gtccccaacg
     2281 tggcgctcat aatgccgccg ccaatcaaca gtacatcagt ttcctgctcc tcggacgctt
     2341 tcgcttttgc cgccatcgaa acggcattaa gccccacggc catcgagaag agcatggcag
     2401 tcactttttt catcttgtta atgccttact tttagtcgct ttattgcagg tgagatttgc
     2461 gcggcatcaa cggtaacact taagtaacaa catttaaata atgtttataa attatattct
     2521 aattttagaa acattataat ttcgttgatt aattataggg ttattagaaa tgaagggatt
     2581 tttagcatat taacctggcg gcagcagtcc gccaggtcag cgtcgtaata ttatgccgtc
     2641 cgggcaacgg catcacgcga agcagcatcg cgctcttcgc ctatcagctc gctaagttgc
     2701 ccgttgcgca tttccagcag gcggtcggcg tggataaagt aatgatcatc atgactgatg
     2761 gcgaaaatcg ttttacccat ctcctgcatc agcggcagca gcacctgata aaactcacga
     2821 cggaagtgtg ggtcctgatc cgccgcccat tcatccagca ggataatatc gcgttcttct
     2881 gccagcgcca gcaacagcgc gacgcgtttt ttctgccctt tcgataactt caggttaaca
     2941 atacgcccgt tgcttaactc aagcttatga gccattttca gctgcaccag ccacttctca
     3001 atcagttgcg ggttagcggg ttgaccctcc ggccccaaca gttgatcaaa cagccagaca
     3061 tcggtaaaca ctgccgaaaa cagttttcga taatcttccg gttgttcgcc gctgacaggt
     3121 ttgccatcca gcaagatttc gccattttgt ggctggtaca agcccgtcag caacatcgcc
     3181 agcgtcgatt ttccactacc gttgccacca atcagaaaca gcagctcgcc acgtttaatg
     3241 gtgaggttaa tcggcccaac ggaaaacgcg ttatcctgat agctaaacgt cacgttacgc
     3301 agctccagcg tttgccagtt gggaaacgcc tgcgggcgtg gaaactctgc tttgaaaggt
     3361 gcgagcgcga atttgttcag cttgttaaac gccacctgcg ccgtcagcag cgtcggtaat
     3421 gcgccaaccg ccgaaagcag cggcgtacgc aggaataaca gcgtcaacga ataggtcgcg
     3481 gcaacgttgg tatcagccca accgagactg ttcgccatcc agaacaccag gccgattgcg
     3541 cccagcatca tgatgtttga ccagttcacg gcactaagat ggaaggtgtc tgcgcggata
     3601 atatgatggc gatactcttg cgcatcagga atgtagaggt tgttaaacac atactcggcg
     3661 cgttcacggt tcagagtcag ctctttgcgc ccttcgagta cggtttgaaa atccgtgtac
     3721 agcttatctt cggtttcacg cagggtcgcc atatgtttgt acacccgcgc caccagtata
     3781 aaaccacccc agatggtgac tgccatccag atagccgtta ccagcaacat tttgcccgac
     3841 agcatccaca aatacgccgc tgaaccgata gtgagaatga tcccctgcac cagttccggc
     3901 agacgcacaa aagcaatggt gatattgcgc acatcgctgg ttaaccccgc cagcaacgag
     3961 gcgctaccaa gttgttcaat gcgctcgacg tgagtatcga gaatccgctt gataaattca
     4021 ctacgcagtc ggtaaacgaa gtgatgcccc aaagtggtga gcgccagttg cgatccgaga
     4081 gtgacggcca tcaacagcag caataacccc agaaactccg gcaataccag cagactggta
     4141 tccgccgttt cgataaggcg ctgattgata aaagcaatta agccaatgcc taatgccgca
     4201 ctggcgaggc ttagcgccat cacactgata aatggccagc gatactgccg ccagacaagt
     4261 acaagaagtt ccatgcagaa aacccggaca atgaattaca gcccgcagtt taaacatctt
     4321 cgtgcgcaca gcaataataa ttcttatttt tattcttttt tacctgcctg acgga
//
LOCUS       DAZKHK010000246         4247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKHK010000246 DAZKHK010000000
VERSION     DAZKHK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.248"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..149
                     /locus_tag="ACHOS5_005046"
     CDS             24..149
                     /locus_tag="ACHOS5_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779705.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGKERRK"
     gene            229..462
                     /locus_tag="ACHOS5_005047"
     CDS             229..462
                     /locus_tag="ACHOS5_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779706.1"
                     /translation="MGINTPTEINKLFTDPAHTNAHITTITRKAERSHICELLRELIE
                     TAKETHTCPQETCAAIERLIAMLTNPDELKECA"
     gene            462..1043
                     /locus_tag="ACHOS5_005048"
     CDS             462..1043
                     /locus_tag="ACHOS5_005048"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP9779707.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFERFIDP
                     VDSERNGGTVNAATAVWWAGQSVDARACLRNADGTELAAVTEFLAFISRNMHDESPGK
                     TLTVWCKGASFDFPILKSAITRTTGDKSIPWCYWNERCMRPLIDMAESTGWRMPGRQG
                     KETIAHTAPNDAIYQAKVVSEIWQRFTAPFLSV"
     gene            1081..2049
                     /locus_tag="ACHOS5_005049"
     CDS             1081..2049
                     /locus_tag="ACHOS5_005049"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFP9779708.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYSRYILGDSNGALINFWRTARDNTDSLIDEAKTLFLNHNNRTDYLAIRNTFNA
                     ENHAFLSTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRTPYFPERE
                     IRAFADKANSTRTLLVHGDFRKTLSCASHLFSMGNTLCVYCDPPYLPFNARDNFTTYG
                     KPFDEGEHVRLRAALDHLSQETGGMTSITISSSDTPEARRIYQGYRMNCIQAPRSVGA
                     RTREPAPEVIATLKQCDAYGRHGGGYCPDCGPAMGYVTYSTMFGNH"
     gene            2085..2315
                     /locus_tag="ACHOS5_005050"
     CDS             2085..2315
                     /locus_tag="ACHOS5_005050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779709.1"
                     /translation="MVNINNTEYGITVLALKGLLLELTELQDDAMDEGNEKLEITLCA
                     TERLIESALDIMEQQQEQIAKVKRAINEMHEE"
     gene            2324..2530
                     /locus_tag="ACHOS5_005051"
     CDS             2324..2530
                     /locus_tag="ACHOS5_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779710.1"
                     /translation="MIQEKTIIRFTTHGHQYEVDESLIDQGMTRRDSRNPDVYHIRLI
                     NGSHFRATNVEEVRVLTTSCRCAL"
     gene            2867..3094
                     /locus_tag="ACHOS5_005052"
     CDS             2867..3094
                     /locus_tag="ACHOS5_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP9779711.1"
                     /translation="MANRKQQRARAERLHTRTEINRRLWRASRVAQIMYINMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            3163..3579
                     /locus_tag="ACHOS5_005053"
     CDS             3163..3579
                     /locus_tag="ACHOS5_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779712.1"
                     /translation="MKNVKGLSDIQDFFNWMWAVEPRLASRLNDWHDYYRRACKRAAR
                     RVTTYGDFSFTVDDRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEITLVREMTNHSV
                     AVKGIYTAEGFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            3576..>4247
                     /locus_tag="ACHOS5_005054"
     CDS             3576..>4247
                     /locus_tag="ACHOS5_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9779713.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLARHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQRLATLTATAFSTALEHACDEVIEKTG
                     RGDDDL"
BASE COUNT         1110 a         1209 c         1109 g          819 t
ORIGIN      
        1 ccttatttca cagaggtggc agcatgagcg tatttctttc atgggtgatt ctgattattt
       61 ccgtgcttat ggcgattggc attatgcgaa ttatcgaagc gctgaaaaat atcgaatctt
      121 tatttactgg caaagaacgc cgtaaataaa taaccaaccg gacagaaaac attaaaacaa
      181 tccgcatctg cggagggctt cactcaatcc agaaacggag aaacagctat gggcatcaat
      241 acacctacag aaataaacaa actatttacc gacccggcgc atacgaacgc gcatatcacc
      301 acgatcacaa gaaaagccga gcgttctcat atttgcgaat tactgcgcga gctgattgag
      361 acagcgaaag agacacacac ctgcccacag gaaacctgcg cagccattga acgactgatt
      421 gcaatgctca ccaacccgga cgaactgaag gagtgcgcat aatgaaccat ctcatgattg
      481 acaccgaaac actgggtaac ggaccggacg cagccatatt cgcgatcggg gcagtgtttt
      541 tcgacccgtt caccggaaag ctgggaaaac agtttgagcg attcattgac ccggtggatt
      601 cagagcgcaa cggtggaacc gtcaatgcag caaccgctgt atggtgggcc ggacaatccg
      661 tggacgccag agcctgcctg cgcaacgcag atggaacaga gctggcagcc gtgaccgaat
      721 tcctggcgtt catttcacgc aacatgcatg acgaaagccc gggcaagaca ctgaccgtat
      781 ggtgcaaggg cgcttcgttc gacttcccga tcctgaaatc cgctatcacg cgaacaaccg
      841 gagataaaag catcccctgg tgttactgga acgagcgctg tatgcgtccg cttatcgaca
      901 tggctgaatc aacaggctgg agaatgccag gcagacaagg aaaagagacc attgctcata
      961 ccgcaccgaa cgacgccatt taccaggcga aggttgtttc agaaatctgg cagcgcttca
     1021 ctgcgccatt cctgagcgta tgaagcggac acacgaaacc tgcaacaaag aggccgcgtt
     1081 atgagcaaag aactgttatc aggcacacca ctgaaatggg tgggcggaaa aagccgcatc
     1141 atgaacacac tgaaggcgca cctgccggaa gctgactgtc tggttgagcc gttcgtgggc
     1201 ggcgcatcgg tattcatgaa caccagttac agccgctaca tcctgggtga ttccaatggt
     1261 gcgctgatta atttctggcg gacagcacgg gacaacaccg acagcctgat cgacgaggcg
     1321 aaaaccctgt tcctgaacca caacaacagg actgactatc tcgccatccg aaacaccttt
     1381 aatgcagaga atcatgcatt cctgagcacg ggaataagcg accgtcggga actgcgactg
     1441 gccgccatgt ttttgtatct caaccggcac tgctttaatg gcctgtaccg ggtgaacgga
     1501 aaaggtgact ttaacgttcc tttcggtggc taccggactc cctacttccc tgaacgggaa
     1561 atccgcgcat ttgcagacaa ggcaaacagc acccgaacac tgcttgttca tggcgatttt
     1621 cgaaaaacgc tgagctgtgc cagccacctg ttcagcatgg gtaacacgct ttgcgtgtac
     1681 tgcgatcccc cttacctgcc tttcaatgcc cgggacaatt tcaccaccta cggaaaacca
     1741 ttcgatgaag gcgaacacgt tcgcctgaga gcagcgcttg accatctttc acaggaaacg
     1801 ggcggcatga ccagcatcac catatccagt tccgacacac cggaagcccg gcgtatttat
     1861 cagggttacc ggatgaactg catacaggca ccacgaagcg ttggcgcaag aacccgcgaa
     1921 ccagcaccgg aggttatcgc caccctgaaa cagtgcgacg catacggtcg ccatggcggc
     1981 ggctactgcc cggactgcgg gccggcgatg ggatatgtaa cttacagcac catgtttggc
     2041 aatcactgaa acagaagcat caaccatcag gaaaggaaag acagatggtg aacatcaata
     2101 acaccgaata cggcatcaca gttctggcat tgaaaggcct ccttctggag ctgacggagc
     2161 ttcaggacga cgccatggat gagggtaacg agaagcttga aatcacgtta tgcgcaaccg
     2221 aacgactgat tgagtctgcg ctggacatca tggaacagca acaggaacaa atcgcaaaag
     2281 tgaagagggc aataaacgaa atgcatgaag agtagggcta accatgattc aggaaaaaac
     2341 catcatccgg ttcacaaccc acggccatca gtacgaagtc gacgaaagcc tgattgacca
     2401 gggcatgaca cgccgggatt cacgcaatcc cgatgtatat cacatccgcc ttatcaacgg
     2461 ttcgcacttc cgcgccacga acgtggaaga agtgcgcgtg cttaccacaa gctgccgctg
     2521 cgctctgtga actgttaccg gactgagtga tcatcatgca cacacaaaaa aaccgcttgc
     2581 cagcgttcgc ctggtcgggt tacagttttc ccgcacctca taaaacgggt gccgggattg
     2641 caacccccgc cgactatgca cgcgcacaac cgcgccagcg gtttttttgt gcgtactgta
     2701 tcgccacgtc tttttcgcat cgaaattatg gcggggcgta cggggccgac ttcggtcggg
     2761 ccgggttcgt gcgtagccgg tggttgcaac cctgtacgtc tcgccaccca gaattgcaac
     2821 ctctggatgg tgagttttca aaacttacta cgtacgaggc cacaccatgg caaaccgcaa
     2881 acaacagcgc gcccgcgctg agcgtctgca cacccggact gaaatcaacc gcagactctg
     2941 gcgcgcatca cgcgtcgcgc aaatcatgta catcaacatg ctgcatgaac acgcacacgc
     3001 gctgtcagac cgttactgtg ccgccgtctt cagctacctg gcggatgacc tggcagagct
     3061 gcaggacctt atcaaccagc aacaccacca ctaaacaaca tcctgaagcc atcccgcacc
     3121 cgtgcgcggg aggcttccgc acgtctgtta ccggaggttc tcatgaagaa cgttaaggga
     3181 ctttcagata ttcaggattt ttttaactgg atgtgggcag tggagccccg gctggcttcc
     3241 cgcctgaacg actggcacga ctattaccgc cgcgcctgta aacgggccgc cagacgcgtc
     3301 accacatacg gggatttctc cttcaccgtt gacgaccgtt accgcgtgga cgtgaaaggt
     3361 gatggtgatg gttttggttt atttcacatc aacggaaccg acagcaccgc catcagctgg
     3421 tcgtcgtcag agataacgct tgtgcgggaa atgacaaacc acagcgtggc cgtgaaaggt
     3481 atatacaccg cagaaggttt tatcggcgaa taccagcgcc ttctcgtgct gtaccgcgcg
     3541 gcgtttcagg cggcgactgc cggggagtgc gacgcatgag tgaccacaat gcacagctgg
     3601 cgtacccgtg gaacgccccg ctaccggtta tcgatcctga aacgttcggc agagcgcagc
     3661 caaccgcatt acgccaggcc attcagagct acatcaacga ggatattcgc attgatgcca
     3721 gactggatga agaaaccgtc gccttcctga caaccacaga cgaagggaag cgaattaatt
     3781 cgcatcttca ccacgacgaa gagcgtcgga tcaggctgga aaaactggcg agacacaaac
     3841 gcgaaaatcc acccacactg gtatctgaag ccatggcaga gctgcgcgcc ctgccgccgt
     3901 ttcttgccgg tccgctcatc cgtgacctgg cacgcctgag ccgcaaacag gagagcgcac
     3961 gccgggaagg gcttaagaat aacagcgcat atgcggcgga taacttcgtt cgtcacgggc
     4021 tgcgtgcccg cctgaaacgt atcgcccgtg tgaatgaacg ttttgccacc ccggccttta
     4081 aggccaccgc cgcccgcgaa cggctggatg aactgcttat gctgccgcag ctgagccgtg
     4141 acgaaatcca gcgccttgcc accctgaccg ccaccgcgtt ttccaccgca ctggagcacg
     4201 catgtgatga ggtcatcgaa aagaccggca ggggtgacga tgacctg
//
LOCUS       DAZKHK010000247         4244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKHK010000247 DAZKHK010000000
VERSION     DAZKHK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.249"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1019
                     /locus_tag="ACHOS5_005055"
     CDS             <1..1019
                     /locus_tag="ACHOS5_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9779714.1"
                     /translation="QLVQVVNAVDVQKQLNALFTSLFFTRSVMFESRDIILDTIDDPN
                     IPIAAFCSPMVGSKVSRDEGYESKTIRPGYMKPKSSIDPNKLAVRPVGVSPEQYNAFG
                     ARNIKVKQAIVNQAKAIRARIEWLAVQAITTGKNIIEGDGIERYELDWNIKPQNIITQ
                     SGGAEWSGKDKETFDPNDDIESYAEFSEGVTNIIIMGGNVWKKYRSFRAIKEALDTRR
                     GSNSELETALKDLGDSVSFKGYMGDVAIVVYSGRYTDEDGTEKHFLDPDLMVLGNTAL
                     QGIVAYGGIQDPELIRMGLTKAELAPKNYIVPGDPAIEYVQTHSAPQPIPARINRFVT
                     VRIG"
     gene            1029..1370
                     /locus_tag="ACHOS5_005056"
     CDS             1029..1370
                     /locus_tag="ACHOS5_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9779715.1"
                     /translation="MATHYTELMAGTEALVTTLGIFSANKGVIPAFTPLMQEDATGAL
                     VVWDGSSVGKAVYVSAVQIDTAKKTQAQVYKTGVLNVDALNWPESVKELSVKVAAFVG
                     SGISVQPLARV"
     gene            1382..1765
                     /locus_tag="ACHOS5_005057"
     CDS             1382..1765
                     /locus_tag="ACHOS5_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9779716.1"
                     /translation="MQNDYNDLKPIAEMMYPNQAVEELKAIADKMCLSERLVDMNQVM
                     EITTLSRRTLLNLEASGEFPERVQVTEGRKAWYLSEVIDWINNIPRASEYCRVPVPKK
                     PDAALCLKIERVRRNARDGRYKLIG"
     gene            1967..2509
                     /locus_tag="ACHOS5_005058"
     CDS             1967..2509
                     /locus_tag="ACHOS5_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9779717.1"
                     /translation="MKLHLMNKKNMAKSCRVSATAFDKWGVIPVERKGREAFYDVASV
                     IDNRVNNAISQITNETGDIDDDELLRVRIRLLTAQAEAQELKNERERGDVIDTEFCMY
                     VLSKLASQISSIMDSLPLTMQRRFPQMTPAMLDGLKKEVAKACNASAGVADNLPQILA
                     DYLMESTGNVPDKLQLNKDK"
     gene            2521..2796
                     /locus_tag="ACHOS5_005059"
     CDS             2521..2796
                     /locus_tag="ACHOS5_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779718.1"
                     /translation="MTESELLKVICHAGGVSHQHDEQATQPGSVTAENYARVVAEVMW
                     RDGIELNGQDCLVIRTKVLAILAARRRQGQRQNVASYQWKKPDRLRR"
     gene            complement(3199..3960)
                     /locus_tag="ACHOS5_005060"
     CDS             complement(3199..3960)
                     /locus_tag="ACHOS5_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFP9779719.1"
                     /translation="MNIIDTLQNDLERAVALQNILIARATGAQDEANNDYLLLRTYFL
                     QNPPLHQLLPEFVRTNRSLKEFWGFIKKTEKYAPRRDLIYKAFQPMLDYLEGKNKAPA
                     DEDISDVLRKFNEDGVHAVWIKALERRHTDPDGAITSARTLLETVCKHILDDMGIQYN
                     NKNIEMSELYKTLSKELNLSADQHTENIFKQILGGCSAVVNGLGTLRNKLGDAHGTNS
                     SSIKPSPRHAELAVNLSGSMALFLINTWLNLKDGK"
     gene            4122..4244
                     /locus_tag="ACHOS5_005061"
                     /pseudo
     CDS             4122..4244
                     /locus_tag="ACHOS5_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein E"
BASE COUNT         1173 a          849 c         1041 g         1181 t
ORIGIN      
        1 cccagcttgt acaggtggtt aatgctgtag atgtacaaaa acaactaaat gcgttgttta
       61 ccagtttgtt ttttactcgc tcggtaatgt ttgaatcgcg cgatattatt cttgatacaa
      121 tcgacgatcc aaatatccca attgcagcgt tttgttctcc tatggtgggt agtaaagttt
      181 cacgtgacga agggtacgaa tcaaaaacaa ttcgtccagg ctatatgaag ccgaaaagca
      241 gcattgatcc aaataagtta gctgtgcgcc ctgttggtgt atcacctgag caatacaatg
      301 cttttggagc gcgtaatatt aaagttaaac aggcgattgt aaatcaggct aaagctattc
      361 gtgcacgtat tgaatggctt gccgttcagg caatcacaac ggggaaaaat atcattgagg
      421 gcgatggtat tgaacgttat gagctggact ggaatattaa accacaaaat atcatcactc
      481 agtctggcgg tgctgagtgg tcaggtaagg ataaagaaac ttttgatcca aatgatgata
      541 ttgagagcta cgcagaattt agtgagggcg tcactaatat tatcattatg ggcggtaatg
      601 tatggaagaa ataccgttca ttcagagcga taaaagaggc tctggatacc cgtcgtggtt
      661 ctaattccga actggaaacg gcccttaaag accttggtga ttcggtgagt tttaaagggt
      721 atatgggcga tgttgcgatt gttgtttaca gcgggcgtta taccgacgag gacggaacag
      781 aaaaacattt ccttgatcct gatttgatgg tgcttggcaa tacggctctt caggggattg
      841 tcgcctatgg cggtattcag gatccggagc taatccgaat ggggctgact aaagccgaac
      901 ttgcaccgaa aaactatatt gtgcctggtg atccggctat tgaatatgtg cagacacatt
      961 cagcaccaca gccaataccg gcccgcatca atcgttttgt taccgttcgc attggctaag
     1021 ggggagcaat ggctactcat tacactgaac tcatggctgg cactgaagca ctggtgacta
     1081 cgctggggat attttcagct aataaagggg taattcctgc atttacgcca ctgatgcagg
     1141 aagatgcaac aggtgcactg gtagtatggg atggttcgag cgtaggtaaa gcggtttatg
     1201 tttccgctgt acaaatcgac accgcgaaaa aaacacaggc tcaggtctat aagacaggtg
     1261 tcttaaatgt tgatgctctg aactggcctg agtctgttaa agaactgtca gtaaaggttg
     1321 cagcgtttgt tggctcaggt atttctgttc agccgctggc tcgtgtgtaa agggggatac
     1381 aatgcagaat gattacaatg accttaagcc aattgccgaa atgatgtacc cgaatcaagc
     1441 tgtagaggaa ttaaaagcta tcgctgacaa aatgtgttta agcgagcgcc ttgttgatat
     1501 gaatcaggtg atggaaatta caaccctgag tcgtcgtaca ctgctaaacc ttgaggctag
     1561 tggagagttc ccggagcgtg tgcaggttac ggaagggcgt aaggcctggt atttaagtga
     1621 agtgatcgac tggataaata atattcctcg cgcttctgaa tattgccgcg tacctgtccc
     1681 aaaaaagcca gatgcggcgc tatgcctcaa gattgagcgt gtacgccgca atgcacggga
     1741 tggtcgctat aagctgattg gttgatgaaa ttagggcctg ctctggctgg cgggtccttt
     1801 ccggcgatct gacaggctac ggggcgtcag gcgcgcgggt tttcgctatt tatgagcatt
     1861 ttaaggggac tggtggtggt tttgttgttc gctatatctg tatgaataat aagagaaaaa
     1921 tacaatcaat acaccaacct gaaacagtaa ttaagttgtg gtatcaatga aattacacct
     1981 gatgaacaaa aaaaacatgg caaaaagttg ccgtgtaagc gccactgcgt tcgataagtg
     2041 gggagtgatt cccgtcgagc gtaagggccg tgaggcgttc tatgatgttg ccagtgtaat
     2101 agataatcgg gttaacaatg caatcagcca gattacaaac gaaacgggcg atattgatga
     2161 cgatgaactt ttacgcgtca ggatcagatt actgacagca caggcagagg cgcaggagct
     2221 taaaaacgag cgcgaacgcg gcgacgttat tgatacagag ttttgtatgt atgttctttc
     2281 caaactggcg agtcagattt catctatcat ggacagcctg ccgcttacca tgcaaaggcg
     2341 cttcccacag atgactccgg ctatgctgga tggacttaaa aaggaagtgg ctaaagcctg
     2401 taatgccagt gccggcgttg ctgacaacct cccacagata ctggctgatt atctgatgga
     2461 aagtacagga aacgtaccgg ataagttgca gctgaataag gataagtaac gtagtacgct
     2521 atgactgaat ccgaactact gaaagtaatc tgccatgctg gtggagtcag ccaccagcat
     2581 gacgaacagg ccacgcagcc gggcagtgtc accgctgaaa attacgctcg tgtggttgct
     2641 gaggttatgt ggcgtgacgg tatagaactg aacggacagg attgcttagt catccgcacc
     2701 aaagtgctgg ctatactggc agctaggcgg aggcagggac aacgccagaa cgttgcatcg
     2761 taccagtgga agaagcctga cagactgcgg cgataactct ttgattttct cgacggcccc
     2821 aaaatgggcc ttaccatagc cagctaataa atgagcaagc ctcaaaagtg aggtttgtgg
     2881 ccgtttggcc tattcatatg ctaactcgtt gatattcctg acgacgcaaa tttacgcctt
     2941 gtggctggta gtcgaattgc aaatttgcaa ctcgactatg aaactacagg tagtttgggt
     3001 agtaaaagca acacactgat tttggggctt cttcgcgata cctaatatta tggtatcggt
     3061 ggaagagata tcgtttctca taggttagca ccgagggcgg agttccgccc tcatcgaaaa
     3121 attgcttacg agtcgtcaaa ataaattaat cgactgagta taatgattaa ctaattgctc
     3181 gtgttacttc attatacgtt atttcccatc tttaaggttt aaccaagtgt taattaaaaa
     3241 aagagccatt gagcctgata aatttactgc aagttcggca tgccttgggg atggcttaat
     3301 tgatgagctg ttcgttccat gtgcatcacc gagtttatta cgaagagtac ctaacccatt
     3361 tacaactgca gaacaaccgc cgagaatttg tttgaagata ttttcagtat gttggtcggc
     3421 agataggtta agttcttttg aaagagtttt ataaagctct gacatttcaa tatttttatt
     3481 attatactga atgcccatat catcaagaat gtgtttgcaa actgtttcaa gaagtgttct
     3541 tgcggaggtt atcgcaccat ccggatctgt atggcgccgt tcaagtgctt ttatccaaac
     3601 ggcgtggact ccatcctcgt taaactttct taatacatca gatatgtctt catccgctgg
     3661 agctttattt ttcccttcta gatagtccag catcggctga aatgccttat aaatcaaatc
     3721 tctccggggg gcatactttt cagtcttttt tataaatccc caaaattctt ttagtgagcg
     3781 attagttctc acaaactctg gtaatagctg atgtaaagga ggattttgta agaaatatgt
     3841 tcttagaagc aaataatcat tattagcttc atcctgggct cctgtagctc ttgcaattag
     3901 tatgttttga agcgctactg cgcgctctag gtcattttga agggtatcta taatattcat
     3961 aacaaatctt tattcacttc atttgtgggt tgataattga atggtttgta cattgatttc
     4021 tgtgtattgc agtgtgtatt gcaacatagc cattcaggta gagattatgc tctatttttc
     4081 ctcttatatc attcacatac ttacaccatt gactcatgta gccgaagcat gaagtgaatc
     4141 cgcagatgac cctgcgtcgc ctgccggatg aagatccgca gaatctggtg gacccggctt
     4201 accgccgccg tcgcatcatc atgcagaaca tgcgtgacga agag
//
LOCUS       DAZKHK010000248         4240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKHK010000248 DAZKHK010000000
VERSION     DAZKHK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.250"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            16..2310
                     /gene="tdcE"
                     /locus_tag="ACHOS5_005062"
     CDS             16..2310
                     /gene="tdcE"
                     /locus_tag="ACHOS5_005062"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP9779720.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            2324..2713
                     /gene="tdcF"
                     /locus_tag="ACHOS5_005063"
     CDS             2324..2713
                     /gene="tdcF"
                     /locus_tag="ACHOS5_005063"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP9779721.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            2785..4149
                     /gene="tdcG"
                     /locus_tag="ACHOS5_005064"
     CDS             2785..4149
                     /gene="tdcG"
                     /locus_tag="ACHOS5_005064"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9779722.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKSIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
BASE COUNT         1033 a         1056 c         1172 g          979 t
ORIGIN      
        1 gagagattat ttttcatgaa ggtagatatt gataccagcg ataagctgta cgccgacgca
       61 tggcttggct ttaaaggtac ggactggaaa aacgaaatta atgtccgcga ttttattcaa
      121 cataactata caccttatga aggcgatgaa tcttttctcg ccgaagcgac gcctgccacc
      181 acggaattgt gggaaaaagt catggaaggc atccgtatcg aaaatgcgac tcacgcgcca
      241 ttggatttcg ataccaatat tgccaccaca attaccgctc atgatgcagg atatattgac
      301 cagccgctgg aaaaaatagt gggtctgcaa acggatgcgc cattaaagcg tgcgctgcat
      361 ccgttcggcg gcattaatat gattagaagt tcattccacg cgtatggtcg tgaaatggat
      421 agcgaatttg aatacatctt taccgatctg cgtaaaaccc ataaccaggg cgtatttgat
      481 gtttactcac cggatatgct gcgctgccgt aaatctggtg tactgaccgg tttaccagat
      541 ggttatggtc gtgggcgtat catcggtgac tatcgccgtg tggcactgta cggcatcagt
      601 tatctggtac gtgaacgcga actgcagttt gccgatctcc agtcccgtct ggaaaaaggc
      661 gaagatcttg aagccaccat tcgtctgcgt gaggaactgg cagaacatcg ccgtgcgctg
      721 ttacagattc aggaaatggc ggcgaaatat ggctttgata tctcccgtcc ggcgcagaat
      781 gcacaagaag ccgtgcagtg gctctacttc gcttacctgg cggcagtgaa atcgcaaaac
      841 ggcggcgcga tgtcgctggg gcgcacggca tcgttcctcg atatctacat tgagcgcgac
      901 tttaaagcag gcgtactcaa tgagcagcag gcacaggaac tgatcgacca cttcatcatg
      961 aagatccgta tggtgcgctt cttgcgtacg ccggaatttg attcgctgtt ctccggcgac
     1021 ccgatctggg cgacggaagt gatcggcggg atggggctgg acggtcgaac cctggtgacc
     1081 aaaaactcct tccgctattt gcacaccctg cacactatgg ggcctgcacc ggaacctaac
     1141 ctgactattc tgtggtcgga agccttgccg attgccttca aaaaatatgc cgcgcaggtg
     1201 tctatcgtca catcctcttt gcagtatgaa aacgacgatc tgatgcgtac tgacttcaat
     1261 agcgacgatt acgcgattgc ctgttgcgtc agcccaatgg tgattggtaa gcaaatgcag
     1321 ttctttggag ctcgcgctaa cctggcgaaa acgctgctgt acgcaatcaa cggcggaatg
     1381 gacgagaagc tgaagattca ggttggcccg aaaacggctc cgttgatgga tgacgtgctg
     1441 gactacgaca aagtgatgga tagcctcgac cacttcatgg actggctggc ggtacagtac
     1501 atcagcgcgc tgaatatcat tcactacatg cacgacaagt acagctacga agcttcgctg
     1561 atggcgctac atgatcgtga tgtctatcgc accatggcgt gcggcatcgc gggcctgtcg
     1621 gtggcaacgg actccctgtc tgccatcaaa tatgctcgtg tgaaaccaat ccgtgatgaa
     1681 aacggcctgg cggtggattt cgagattgac ggcgaatatc cgcagtatgg caacaacgac
     1741 gagcgcgtag acagcattgc ctgcgatctg gttgaacgct ttatgaagaa aattaaagct
     1801 ttgccaacct atcgcaatgc tgtgccgacc caatcgattc tgactatcac ctctaacgtg
     1861 gtgtacggtc agaaaaccgg gaacacgccg gacggtcgcc gcgccggaac accgttcgcg
     1921 ccgggcgcta acccgatgca cggtcgtgat cgcaaaggcg ccgtggcctc attgacgtcg
     1981 gtggcgaaac tgccgttcac ctacgccaaa gatgggatct cgtacacctt ctccatcgtc
     2041 cctgcggcgc tgggcaaaga agatccagta cgtaaaacca accttgtcgg cctgctggat
     2101 gggtatttcc accacgaagc ggatgtcgaa ggcggtcaac acctcaacgt caacgtaatg
     2161 aatcgggaaa tgctgctgga tgccatcgag cacccggaaa aatatcctaa cctgacaatc
     2221 cgtgtctctg gctacgccgt gcgctttaac gcactgaccc gtgaacagca acaggatgtt
     2281 atttcacgta cctttaccca ggcgctctga cgccggagga tgtatgaaaa agattatcga
     2341 aacgcaacgt gccccaggcg caatcggccc ttatgttcag ggcgttgatt taggcagcat
     2401 ggtcttcacc tccgggcaaa ttccggtttg cccacagacc ggtgagatcc cggctgatgt
     2461 gcaagatcag gcgcgtttaa gcctcgaaaa cgtcaaagcg atcgtggttg ctgccgggct
     2521 gagtgtgggc gatatcatca agatgaccgt gttcatcacc gatctgaatg attttgccac
     2581 catcaatgaa gtctataagc agttcttcga tgaacatcag gcgacctacc cgacccggag
     2641 ctgtgtgcag gtcgcgcgtt tgccgaaaga tgtgaagttg gaaattgaag ccatcgcagt
     2701 acgtagtgcg taaggcctcg tgagcgggac ggtcgcaagg tcgttccgct ccacttcact
     2761 gaacggcaat ccgagggtgt ggatatgatt agtgcattcg atattttcaa aattgggatt
     2821 ggtccctcca gttcgcatac tgtggggcca atgaatgccg gaaaaagttt tattgatcgg
     2881 ctggaaagta gcggcttatt aaccgcgacg agccatattg tggtcgatct gtacgggtcg
     2941 ttgtcactga cgggcaaagg ccatgccacg gatgtcgcca tcatcatggg actggcagga
     3001 aacagtccgc aggatgttgt cattgatgag atccctgcat ttatagagtt agtgacgcgc
     3061 agcgggcggt tgccggtggc ttctggcgcg catattgttg attttcctgt agcaaagagc
     3121 attatcttcc atcccgaaat gttgcctcgc catgagaacg gaatgcggat cactgcctgg
     3181 aagggacagg aagagctatt aagcaaaacc tattactctg tcggcggcgg gtttattgtc
     3241 gaagaagaac acttcggcct gtcgcacgat gtcgaaacac ccgtacctta cgatttccac
     3301 tcagcaggtg aactgctgaa aatgtgtgat tacaacggcc tgtctatatc tggcctgatg
     3361 atgcacaacg agctggcgct gcgcagtaaa gcggaaattg acgcagggtt tgcccgtatc
     3421 tggcaagtga tgcatgacgg tattgaacgt ggaatgaata ctgaaggcgt gctgcctggt
     3481 ccactcaatg tgccgcgccg tgccgtagcg ctgcgtcgtc aactggtttc cagcgataac
     3541 atctctaacg atccgatgaa tgtcatcgac tggatcaaca tgtacgcgct ggcggttagt
     3601 gaagaaaacg cagctggcgg gcgcgtggta acggcaccga ctaacggtgc gtgcggcatt
     3661 attccggcag tgctggctta ttacgataag ttccgtcgtc cggtaaacga gcggtcaatt
     3721 gcccgctatt ttctggcagc gggggcaatt ggcgcgctgt ataaaatgaa cgcctccatc
     3781 tctggcgcgg aagtcggctg tcagggggag attggcgtgg cctgttcaat ggcggcggca
     3841 gggttaactg aactactggg cggcagtccg gcgcaggtat gcaatgcggc ggaaatcgcg
     3901 atggagcata accttgggct gacctgcgat ccggttgccg gacaggtaca aatcccgtgc
     3961 attgaacgta atgccattaa tgccgtgaaa gcagtaaacg ccgcgcggat ggcaatgcgc
     4021 cgcacctcgg caccgcgtgt ttcactcgat aaagtgatcg agacgatgta tgaaaccggc
     4081 aaagatatga acgataaata ccgcgaaaca tcacgcggag gactggctat taaagtggtc
     4141 tgcggctgac attgctgtca gctatcatcc ttgggtgcac aaaatgtgca ccctttttta
     4201 ttgtgatctg ccgcaaatag agaataatta ttcctattaa
//
LOCUS       DAZKHK010000249         4196 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKHK010000249 DAZKHK010000000
VERSION     DAZKHK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.251"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            260..1363
                     /gene="ompC"
                     /locus_tag="ACHOS5_005065"
     CDS             260..1363
                     /gene="ompC"
                     /locus_tag="ACHOS5_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9779723.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1475..2530
                     /gene="apbE"
                     /locus_tag="ACHOS5_005066"
     CDS             1475..2530
                     /gene="apbE"
                     /locus_tag="ACHOS5_005066"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP9779724.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDNF
                     KTWMSPQFKSFLISEKN"
     gene            2604..3668
                     /gene="ada"
                     /locus_tag="ACHOS5_005067"
     CDS             2604..3668
                     /gene="ada"
                     /locus_tag="ACHOS5_005067"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP9779725.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVCTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGVSVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSALADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3668..>4196
                     /gene="alkB"
                     /locus_tag="ACHOS5_005068"
     CDS             3668..>4196
                     /gene="alkB"
                     /locus_tag="ACHOS5_005068"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP9779726.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVV"
BASE COUNT         1025 a         1075 c         1106 g          990 t
ORIGIN      
        1 attttaacct tgaattaata ttgcttgatg ttaggtgctt atttggccat tccgcaataa
       61 tcttaaaaag ttcccttaca tttacatcct gaaacatcta tagcgataaa tgaaacatct
      121 taaaagtttt agtatcatat tcgtgttgga ttattctgca tttttgggga gaatggactt
      181 gccgactgat taatgagggt taatcagtat gcagtggcat aaaaaagcaa ataaaggcat
      241 ataacagagg gttaataaca tgaaagttaa agtactgtcc ctcctggtcc cagctctgct
      301 ggtagcaggc gcagcaaacg ctgctgaagt ttacaacaaa gacggcaaca aattagatct
      361 gtacggtaaa gtagacggcc tgcactattt ctctgacgac aagtctgtag atggcgacca
      421 gacctacatg cgtcttggct tcaaaggtga aactcaggtt actgaccaac tgaccggtta
      481 cggccagtgg gaatatcaga tccagggcaa cagcgctgaa aacgaaaaca actcctggac
      541 ccgtgtggca ttcgcaggtc tgaaattcca ggatgtgggt tctttcgact acggtcgtaa
      601 ctacggcgtt gtttatgacg taacttcctg gaccgacgta ctgccagaat tcggtggcga
      661 cacttacggt tctgacaact tcatgcagca gcgtggtaac ggcttcgcga cctaccgtaa
      721 cactgacttc ttcggtctgg ttgacggcct gaactttgct gttcagtacc agggcaaaaa
      781 cggtagcgta agcggcgaag gcatgaccaa caacggtcgt gatgctctgc gtcagaacgg
      841 cgacggcgtt ggcggttcta tcacttatga ttacgaaggc ttcggtatcg gtggtgcgat
      901 ctccagctcc aaacgtactg atgctcagaa caccgctgct tacatcggta acggcgaccg
      961 tgctgaaacc tacactggtg gtctgaaata cgacgctaac aacatctacc tggctgctca
     1021 gtacacccag acctacaacg caactcgcgt aggttccctg ggttgggcga acaaagcaca
     1081 gaacttcgaa gctgttgctc agtaccagtt cgacttcggt ctgcgcccgt ctgtagcata
     1141 cctgcagtct aaaggtaaaa acctgggcac catcggtact cgtaactacg acgacgaaga
     1201 tatcctgaaa tatgttgatg ttggtgctac ttactacttc aacaaaaaca tgtccaccta
     1261 tgttgactac aaaatcaacc tgctggacga caaccagttc actcgtgacg ctggcatcaa
     1321 cactgataac atcgtagctc tgggtctggt ttaccagttc taatctcgat ggatatcgaa
     1381 caaagggcct gcgggccctt ttttcattgt tttcagcgta caaacacagt tttttggtgt
     1441 actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta cccgcgtggc
     1501 actgctggct gccgcgctct tctttgttgg ttgcgatcaa aaaccacaac ccgccaaaac
     1561 ccacgctact gaagttaccg ttcttgaagg caaaaccatg ggtaccttct ggcgtgccag
     1621 cattccgggc attgacgcca aacgcagtgc cgaacttaaa gaaaagattc agacccagct
     1681 ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga tgcgctttaa
     1741 cgactcgcaa agtttgtcgc cgtggccggt aagtgaagcg atggccgata tcgtcaccac
     1801 ctcgctgcgc attggcgcaa ggaccgatgg tgcgatggat ataaccgtcg ggccgctggt
     1861 gaatctgtgg ggctttggtc cggaacaaca accggttcaa attccgagcc aggaacagat
     1921 cgatgcgatg aaagccaaaa ccggcttaca gcacctgacg gtcattaatc agtcgcatca
     1981 gcaatatctg caaaaagacc tgccggattt atatgtcgat ctctccaccg tcggcgaagg
     2041 ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa gggatttccc gctatctggt
     2101 gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt gcaggccagc cgtggcgggt
     2161 agcgattcaa aagccaaccg ataaagaaaa cgcggttcag gccgtggtgg atatcaacgg
     2221 tcatgggatt agtacctctg gcagctaccg taactattac gaactggacg gcaaacgtct
     2281 ttctcatgtt atcgatccgc aaaccgggcg tcctatcgaa cacaatctgg tatccgtgac
     2341 ggtgattgcc ccgacggcgc tggaggccga tgcctgggat actggcttaa tggtgctcgg
     2401 gccggagaaa gccaaagagg ttgttcgccg ggaagggctg gcggtctaca tgatcaccaa
     2461 agagggcgat aattttaaaa cctggatgtc gccgcagttt aaaagcttcc ttatcagcga
     2521 aaaaaattaa agcgcaagat tgttggtttt tgcgcgatgg tgaccgggca gactaacggc
     2581 tatccttaac cagggagctg attatgaaaa acgccacatg tttaactgac gatcaacgct
     2641 ggcaatctgt cttagcccgc gacccgaacg ccgacggcga attcgttttc gccgtgtgca
     2701 ccacgggtat cttttgccgt ccgtcttgcc gagccagaca tgccttgcgg gaaaatgtct
     2761 ccttctacgc aaatgccagc gaggcgcttg ccgctggctt tcgtccctgc aaacgttgtc
     2821 agccagacaa agccaatccc ctgcaacatc gccttgataa aattacccac gcctgccgac
     2881 ttctggaaca ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg gcgatgagtc
     2941 catttcattt gcatcggttg tttaaagcga ctaccggaat gacgcctaaa gcctggcaac
     3001 aggcctggcg cgctcgccgt ttgcgcgaat tgctggcgaa aggggtgagc gtgactacat
     3061 ctattcttaa cgccggattc cccgacagca gcagttacta ccgcaaagcc gacgaaacgc
     3121 tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg cgttacgcgc
     3181 tggctgattg tgagctgggc cgttgcctgg tggcagaaag tgagcggggg atttgcgcga
     3241 tattactggg cgatgatgac gcgacactaa tcagcgagtt acagcagatg tttcccgctg
     3301 ccgacagcgc gcttgctgat cttacgtttc agcaacatgt gcgtgaagtg atcgccagcc
     3361 tcaatcaacg cgatacgccg ctgacgttac cgctggacat tcgcggcact gcttttcagc
     3421 aacaagtctg gcaggcactg cgcacgatac cttgcggtga aaccgtcagt tatcagcaac
     3481 tggctaatgc catcggcaaa ccgaaagcgg tacgggccgt tgccagtgcc tgtgccgcca
     3541 acaagctggc tatcgttata ccttgtcatc gggtggtccg tggtgatggc actctttccg
     3601 gttaccgctg gggcgtgtcg cgcaaagcgc aactgctgcg ccgcgaagct gaaaatgagg
     3661 aaaggtaatg ttggatctgt ttgctgatgc tgaaccgtgg caagaaccac tggcggctgg
     3721 agcagtgatt ttgcaccgtt ttgcttttaa cgctgcggag caactgatcc gcgatattaa
     3781 tgacgttgcc agccagtcgc cgtttcgcca gatggtcact ccaggcggat ataccatgtc
     3841 ggtggcgatg accaactgtg ggcatctggg ctggacgacc catcggcaag gttatctcta
     3901 ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc atgccgcaga gttttcatca
     3961 tttatgtcaa cgtgcggcta cggcggcggg ctatccagat ttccagccag atgcctgttt
     4021 gatcaaccgc tacgttcctg gcgcgaaact gtcgctgcat caggataaag acgaaccgga
     4081 tctgcgcgcg ccaattgttt ctgtttctct gggcttaccc gcgatttttc aatttggcgg
     4141 cctgaaacga aatgatccgc tcaaacgttt gttgttggaa catggcgatg tggtgg
//
LOCUS       DAZKHK010000250         4185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKHK010000250 DAZKHK010000000
VERSION     DAZKHK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.252"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..145)
                     /locus_tag="ACHOS5_005069"
     CDS             complement(<1..145)
                     /locus_tag="ACHOS5_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9779727.1"
                     /translation="MSRVAAIIYALVICIIVCLSWAVNHYRDNAIAYKALRDKNAREL
                     KLAN"
     gene            complement(142..675)
                     /locus_tag="ACHOS5_005070"
     CDS             complement(142..675)
                     /locus_tag="ACHOS5_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9779728.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            781..1053
                     /locus_tag="ACHOS5_005071"
     CDS             781..1053
                     /locus_tag="ACHOS5_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779729.1"
                     /translation="MDNSTISLQELLDCISRLRDDVNALTVAFSYLAFSIPKEQMQPT
                     LASLQLESINPKWSQQQQNSFKWLAVLLEEKYAGEITISAESSVNQ"
     gene            complement(1019..1363)
                     /locus_tag="ACHOS5_005072"
     CDS             complement(1019..1363)
                     /locus_tag="ACHOS5_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP9779730.1"
                     /translation="MVQNYEMIVKGIRNFENKVTVTLALQDKERFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVATGLCGGDEQSPEKRPIILEAQSVWITYKGKLPG
                     RITGSLKTPPKW"
     gene            complement(1368..1583)
                     /locus_tag="ACHOS5_005073"
     CDS             complement(1368..1583)
                     /locus_tag="ACHOS5_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9779731.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2677..3738)
                     /locus_tag="ACHOS5_005074"
     CDS             complement(2677..3738)
                     /locus_tag="ACHOS5_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9779732.1"
                     /translation="MKNTVKINSIDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYRGPYRGKSDGYVAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELET
                     ERFNQTIQEISMLSANTIL"
     gene            complement(3888..4082)
                     /locus_tag="ACHOS5_005075"
     CDS             complement(3888..4082)
                     /locus_tag="ACHOS5_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9779733.1"
                     /translation="MLKQQDMTETAKVVFNELNGKPATVGEIAQNTYLSRERCQLILT
                     QLVMAGLADYQFGCYRRLQQ"
BASE COUNT         1099 a         1087 c          885 g         1114 t
ORIGIN      
        1 cgttcgccag cttcagttct ctggcatttt tgtcgcgcag ggctttgtag gcgattgcgt
       61 tatcacggta atgattaaca gcccatgaca ggcagacgat gatgcagata accagagcat
      121 aaataatcgc ggcgactctg ctcactgatc tatcccccaa caggctaatg cgctttcctg
      181 gtcacgacga ataacctgtc catagcagtt atttgaacgt atgcggcaat cgcgcccacc
      241 atcttttatc caccagcgaa tcgcctcgca tgcaccttta cgatcaccgg cattcagccg
      301 cttataaaac gtcgacggga aacacttacc ggggccaatg ttatagggac aaaatgacgc
      361 gatacccgct ttctgtggtt cggtcagtgg cactttaata ttgcgctcca cccatgccag
      421 cgccttatcc cgttcaatgg cgttaacctg gtcgcatttt tccttcgaca gtttcatacc
      481 gggaacaacg ggttttccat ccaccaccgt ggcaccccga cagatggtcc agatgccgga
      541 accatcgcgg tatgccgttg tgtggttacc ttctttttca tccagaaact ggtcgagaat
      601 atcaggcgca ggcgcaccga cggcaatcag tgccagaacg gcagccgaca ggccgtatct
      661 gatttttgcg ttcatggata tttatcagga tttatcggtt tctgaaccct ggatatgttt
      721 atctgtcccg gcctgttgaa tcaggcaagg aatagttaaa tacaatagag aggattgttt
      781 atggacaata gcaccatttc tctacaggag ttgctcgact gcatttccag gcttcgggat
      841 gatgtaaatg cccttactgt cgcattttca tatctggcat tctcaattcc caaggaacaa
      901 atgcaaccaa cactggcatc gctccagctt gaatcaatca atcccaaatg gtcccagcaa
      961 caacaaaatt ctttcaagtg gctggcggta ttactggagg aaaaatatgc tggtgaaatt
     1021 accatttcgg cggagtcttc agtgaaccag taattcttcc cggcagtttt cctttgtagg
     1081 ttatccacac actctgcgcc tctaaaatta tggggcgctt ttccggcgac tgctcatccc
     1141 ctccacataa cccggtagca acatccagga agacctgtct gatgctcctt ctggctgctg
     1201 cctcataaaa ctccagcgcg gcaccttcaa cacggtccag cgagatgtcc aggtcaaaaa
     1261 tttcaccgtc aaagcgttct ttgtcctgta atgctaaagt taccgtaact ttattctcaa
     1321 aattgcggat ccctttcaca atcatttcat agttttggac cattatatta ctctcctctc
     1381 gcagcctttc ttctgtcttc tctgattttg aagtacagat tcgtcagata agtcagaaag
     1441 cccagaacca gacttcccag tacaccaatc gcagcccact gagacggact aacctgatca
     1501 agccattgta aaaaccagta gccagcactg cctgcggagg tgccgtaggc aatgcctgtt
     1561 gatatttttt ccatctgata catatcccgc cccaacagac ctgtgctacc ggaaagaaaa
     1621 aaggccatca gcaaaactct gatggcctga atcaccttta ccagtcatgt atgaaaaaat
     1681 acgcacaacc aattgacaat aattatcatt ttcattaaaa ataccgcgta acattcttat
     1741 ttcatgaaga accatacccg ccagcaacct gagtagcaaa agctgttcat cccccatatt
     1801 tttgctggcg ggttcttttt tctactgccc tcctccccag gaaaagcatt tcattctttt
     1861 ctcattaatc gttactggag agaaggcggg aaaaagccag ctctttggcc ctaaataaca
     1921 gcatggcttc agtccataca gaatctgatt ttccagctca cataataacc agatgatgca
     1981 gttgcaaaat ctggcaacat cggattatta tgcgcctgtg aaaatttctt cacgttttat
     2041 tccttgccgc ccgtgcctcc cagcgcgggc ttttttatcc aaaaaatacc cctccggaga
     2101 ggggtatcat acaaggggat gaccatgatg catggtgccg ggtgcctccc ggtgagtccg
     2161 gcatcagcat acgaacccgc gattatccca gaactgactg ctgattcgcc ccgccgctta
     2221 gggggattca ccatgcgttt ttttatttac aaactcttcg ccagacaaac aaccattaac
     2281 tggctgaatt gtgaggtatt tcaattttta gaaaactgac caacctccgc ttatacgccg
     2341 ggcatggagc ggatacaaaa aagccccgca aaagcagatc agaaggattc gttgtaaaaa
     2401 aaaagctgtc agattatcgc gctaatctga cagctacagc aggtatccaa aaacatgggt
     2461 ggaaacaata agtgagttaa gaagacacgg tgtcggattt ttaccgatga gccgttggcg
     2521 tggtcaaccg tgactcgctt gtatcaacga tgaattaatg aagccaatcc tttgcaggct
     2581 atgtacaacc caccacatca gccctgctgc attgcaggat acactgtatc tgtaatcatt
     2641 atacggcaca gacatcccac caataattga attaattcac aaaattgtat ttgctgataa
     2701 catactgatt tcctggatgg tctgattaaa ccgctctgtc tcaagctcaa cacctaacgc
     2761 ccgacgcccc agcgccattg ctgcttttat tgtggaaccg gatcccataa agaaatcagc
     2821 aaccagatcg ccgggtcgac tactggcatt gattatttgc ctgagcatat ccgccggttt
     2881 ctcacacgga tgtttacccg ggtagaactg aacgggttta tgcatccaga catcggtata
     2941 aggcacggag actgatacgg agaaatagcg ccggagagat ttaaactcat ccagcaattc
     3001 agaatatttg cgattcagtg aatcataaga tgccaccaac tggtggtgtg gttgttccag
     3061 ttgttgttcc tgaaacttct ctgccgctat acgggaaaac agtgcctgta acttccggta
     3121 gtcagcctca ttcggcaact gccactgact ggcaccaaac cagtgggaaa ccatattttt
     3181 cttacctgtg gcttcggcaa tttgttttgc cgttataccc agttcggcac gagcatccct
     3241 gaaatacgat atcagcggtg ccattatgtg ctgtttgagt tccctttctt ttgccacata
     3301 gccgtcactt ttgccgcgat atggcccccg gtaatgttca gcaaacagaa cgcgctctgt
     3361 ggcaggaaaa tatgcgcgca gactttcttt attacaccca ttccaacgtc cggacggctt
     3421 cgcccagatg atatggttaa gcacgttgaa acgttcacgc atcatgatct caatatcaga
     3481 tgccaggcga tgcccacaga acaggtaaag gcttccggca ggtttcaaca cccgccagaa
     3541 ctgggccaga cagtggtcca gccacttaag gtaatcttcg tcccctttcc actgattgtc
     3601 ccaaccgttg ggtttcacct tgaagtaagg cggatcggta acaatcaggt caatggaatc
     3661 atcaggcagg gactgaataa aatgcaggca atcagcgttg attaaatcaa tactgtttat
     3721 ttttacagtg tttttcatgg atcagtaagc gtaactctgg taggctcact ctgcttttgc
     3781 gctaaagcag tgggccatgg ttcgcttgtg accagtaagc atgagcgaat ggctggcagg
     3841 tgctaccaac acccaccagc cgcccatttt cacaaattaa aagcccttca ttgctgaagg
     3901 cgtctgtaac agccgaactg gtaatctgcc agccccgcca taaccagctg ggtcagtatt
     3961 aactgacagc gttcgcgtga aaggtatgtg ttttgtgcta tctccccgac tgttgccggt
     4021 ttgccgttta attcattaaa aacaactttc gccgtttctg tcatatcttg ctgttttagc
     4081 atgtcttttt accttcatgg ttaacatgac ataccaataa ctcttgtcta aaaagccagc
     4141 aagataaaaa gtcagtattc acgaccacca gcgtgtttac cgtac
//
LOCUS       DAZKHK010000251         4146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKHK010000251 DAZKHK010000000
VERSION     DAZKHK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.253"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..665
                     /locus_tag="ACHOS5_005076"
     CDS             <1..665
                     /locus_tag="ACHOS5_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9779734.1"
                     /translation="PEGERYFPCVNISGEPEDYFRMAPEDWLQAEMQGEIVALVHSHP
                     GGLPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHGVTDCYTLFRDAY
                     HLAGIEMPDFHRGDDWWRHGQNLYLDNMEATGFYRVALTEAQPGDVLLCCFGSSVPNH
                     AAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFTGIYNDLAAAST
                     FV"
     gene            662..1234
                     /locus_tag="ACHOS5_005077"
     CDS             662..1234
                     /locus_tag="ACHOS5_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9779735.1"
                     /translation="MRTGAEAIRALATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1295..>4146
                     /locus_tag="ACHOS5_005078"
     CDS             1295..>4146
                     /locus_tag="ACHOS5_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9779736.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNANISGVTVVFRAGEQEQTPPEGVESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKKAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITTTPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGNITESHLGKELLEKVDLTEDNASKLEQFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTP"
BASE COUNT          893 a         1055 c         1349 g          849 t
ORIGIN      
        1 cgccggaggg ggaaagatat tttccctgcg tgaatatttc cggtgagccg gaggattatt
       61 tccggatggc tccggaggac tggctgcagg cagaaatgca gggtgagatt gtggcgctgg
      121 tccacagtca tcccggtggt ctgccctggc tgagtgaggc tgaccggcgg ctgcaggtgc
      181 agagtgattt gccgtggtgg ctggtctgcc ggggggcgat tcacaagttc cgctgtgtgc
      241 cgcatcttac cgggcggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg
      301 atgcttacca tctggcgggg attgagatgc cggattttca tcgcggggat gactggtggc
      361 gtcacggtca gaatctctat cttgacaata tggaggcgac tggtttttac cgtgtcgcac
      421 tgacagaggc gcagcctggc gacgtgctgc tgtgctgctt tggttcatcg gtgccgaatc
      481 atgccgccat ttactgtggt gacggcgagc tgctgcacca tattcctgaa caactgagca
      541 aacgagagag gtataccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg
      601 catggcgcgc atctgccttt acggggattt acaacgattt ggccgccgca tcgaccttcg
      661 tgtgagaacg ggggctgaag ccatccgcgc actggccaca cagctcccgg cgtttcgtca
      721 gaaactgagc gacggctggt atcaggtgcg tattgccgga caggatgtca gcacgtccgg
      781 attaacggcg cagttacatg aggctctgcc tgacggcgct gtgattcata ttgttcccag
      841 agtcgccggg gccaagtcag gtggtgtatt ccagattgtc ctgggagcag ccgccattgc
      901 cggatcattc tttaccgccg gagccaccct tgcagcatgg ggggctgcca ttggtgccgg
      961 tggtatgacc ggcattctgt tttctctcgg tgccagtatg gtgctcggcg gtgtggctca
     1021 gatgctggca ccgaaagcca ggacgcccac ggcagccagt acagataacg gcaaacagaa
     1081 cacgtatttc tcgtcactgg ataacatggt tgcccagggt aatgttctgc ctgttctgta
     1141 cggggaaatg cgcgtggggt cacgtgtggt ttctcaggag atcagcacgg cagacgaagg
     1201 ggatggtggt caggttgtgg tgattggtcg ctgatgcaaa atgttttatg tgaaaccgcc
     1261 tgcgggcggt tttgtcgttt atggagcgtg aggaatgggt aaaggcagca gtaaggggca
     1321 taccccgcgc gaagcgaagg acaacctgaa gtccacgcag ctgctgagtg tgatcgatgc
     1381 catcagtgaa gggccgattg aaggtccggt ggatggatta aaaagcgtgc tgctgaacag
     1441 tacgccggtg ctggacagtg aggggaatgc caatatatcc ggcgtcacgg tggtattccg
     1501 ggcaggtgag caggagcaga ctccgccgga gggggttgaa tcctccggtt ccgagacggt
     1561 gctgggtacg gaagtgaaat atgacacgcc gatcacccgc accatcacgt cggcaaacat
     1621 cgaccgtctg cgctttacct tcggtgtgca ggcactggtg gaaaccacct caaaggggga
     1681 caggaatcca tcggaagtcc gcctgctggt tcagatacag cgtaacggtg gctgggtgac
     1741 ggaaaaagac atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtggt
     1801 ggataacctg ccgccgcgcc cgtttaatat ccggatgcgc aggatgacgc cggacagcac
     1861 cacagaccag ctgcagaaca aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa
     1921 acagtgctac ccgaacacgg cactggttgg cgtgcaggtg gactcggagc agttcggcag
     1981 ccagcaggtg agccgtaatt atcatcttcg cgggcgcatt cttcaggtgc cgtcgaacta
     2041 taacccgcag acgcggcaat acagcggtat ctgggacgga acgttaaaac cggcatacag
     2101 caacaacatg gcctggtgtc tgtgggatat gctgacccat ccgcgctacg gcatggggaa
     2161 acgtcttggt gcggcggatg tggataaatg ggcgctgtat gtcatcggcc agaattgcga
     2221 ccagtcggtg ccggacggct ttggcggcac ggagccgcgc atcacctgta acgcttacct
     2281 gaccacacag cgcaaggcgt gggatgtgct cagtgatttc tgctcggcga tgcgctgtat
     2341 gccggtatgg aacgggcaga cgctgacgtt cgtgcaggac cgacagtcgg ataaggtgtg
     2401 gacctataac cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt
     2461 cagcgccctg aaggaccgcc ataatgccgt tgaggtgaac tggattgacc cgaacaacgg
     2521 ctgggagacg gcgacagagc ttgttgaaga tacgcaggcc attgcccgtt acggtcgtaa
     2581 tgtcacgaag atggatgcct ttggttgtac cagtcggggg caggcacacc gagccgggct
     2641 gtggctgatt aaaacggaac tgctggaaac gcagaccgtg gatttcagcg tgggcgcaga
     2701 agggcttcgc catgtgccgg gcgatgttat tgaaatctgc gatgatgact atgccggtat
     2761 cagcatcggt ggtcgcgtgc tggcggtgaa cagccagacc cggacgctga cgctcgaccg
     2821 tgaaatcacg ctgccatcct ccggcaccac gctgataagc ctggttgacg gtgagggtaa
     2881 tccggtcagc gtggaggtcc agtccgtcac cgacggcgtg aaggtgaaag tgagccgtgt
     2941 tcctgacggc gttgctgaat acagcgtgtg ggggctgaag ttgccgacgt tgcgccagcg
     3001 cctgttccgc tgtgtgagta tccgtgagaa cgacgacggc acgtatgcca tcactgccgt
     3061 gcagcatgtg ccggaaaaaa aggccatcgt ggataacggg gcgcactttg acggcgacca
     3121 gagcggcacg gtgaatggtg tcacgccgcc agcggtgcag cacctgaccg cagaagtcac
     3181 cgcagacagc ggggaatatc aggtgctggc gcgctgggac acgccgaagg tggtgaaggg
     3241 ggtgagtttt atgcttcgcc tgaccgtggc cgcggatgac ggcagtgagc ggctggtcag
     3301 tacggccagg acgacggaaa ccacataccg cttcaggcaa ctggcgctgg ggcgttacac
     3361 gctgacggtc cgggcggtaa atgcgtgggg acagcagggc gatccggcgt cggtatcgtt
     3421 ccggattgcc gcaccggcag cgccgtcgcg gattgagctg acgccgggct attttcagat
     3481 caccaccacg ccgcatcttg cggtttatga tccgacggta cagtttgagt tctggttctc
     3541 ggaaaagcgg attgcggata tcaggcaggt tgaaaccaca gcccgctacc ttggcacggg
     3601 gctgtactgg atagccgcca gtatcaatat caaaccgggc catgattatt acttttatat
     3661 ccgcagtgtg aacaccgttg gcaaatcggc attcgtggag gccgtcggtc gggcgagcga
     3721 tgatgcggaa ggttacctgg attttttcaa aggcaatata accgaatccc atcttggtaa
     3781 agagctgctg gaaaaagtcg acctgacgga ggataacgcc agcaaactgg agcagttttc
     3841 gaaagagtgg aaggatgcta acgataagtg gaatgccatg tggggcgtca aaattgagca
     3901 gaccaaagac ggcaaacatt atgtcgcggg tattggcctc agcatggagg acacggagga
     3961 aggcaaactg agccagtttc tggttgccgc caatcgtatc gcgtttattg acccggcaaa
     4021 cgggaatgaa acgccgatgt ttgtggcgca gggcaaccag atattcatga acgacgtgtt
     4081 cctgaagcgc ctgacggccc ccaccattac cagcggtgga aatccaccgg cattttccct
     4141 gacacc
//
LOCUS       DAZKHK010000252         4101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKHK010000252 DAZKHK010000000
VERSION     DAZKHK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.254"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            710..889
                     /locus_tag="ACHOS5_005079"
                     /pseudo
     CDS             710..889
                     /locus_tag="ACHOS5_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1064..3034
                     /locus_tag="ACHOS5_005080"
     CDS             1064..3034
                     /locus_tag="ACHOS5_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFP9779737.1"
                     /translation="MYMNVIRTVICTLIILPVGLQTATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLEPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNVASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYAPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            3053..3844
                     /locus_tag="ACHOS5_005081"
     CDS             3053..3844
                     /locus_tag="ACHOS5_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFP9779738.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1232 a          916 c          969 g          984 t
ORIGIN      
        1 aaaggtaact ctggcggcca tagtgatgac aacgacaggt aagaccggga ctcactaaac
       61 ctgaatattg tgttgagcta aaagctcgct ggagaaatga ggtgtgagtc agcgaattac
      121 ataggggctc gcagcttcat ggctgcaaaa aagccggata taaagctgca acctacccgt
      181 cactgtcaag gcaatggatg ccttgcaacg ggatgtgttc atataatcaa tatgtcagat
      241 ctatgctgat acagggtgcc agagcggtat tattcagagc ctcagagaaa actgattcac
      301 ttcgtgtgtg ggttaattgt attcgggaaa agcgtggctt caaccgggca gttgttgctc
      361 ttgcaaataa actccttcgg atcgcctggg caattatcgc ccgcaatgag atgtattttc
      421 ccgcagcaaa acaaaatcaa taatctacgt aaaatcagta ggagtaaagc ttaccctgaa
      481 attgcaaaga atgcagaatg atgaaaaaca ggtcagaccg acgttacgaa aacctgcaag
      541 atgagccagg ccagtaaggc cgagtaagcg ataaggtcgg agcgcgcgaa tgtctcatca
      601 aggccagaag caataacttc atgaacaaac cggatatacg aaagtaaacc tgtccctatc
      661 atagttgctg tactcttgca atacggggga agatcatctc atcacttacg aacgattcgt
      721 gaaaacgatg aagaaagact acatcgcgtg catgccgaaa tctaatgtaa gaacggcatt
      781 gcataatctt gcagtggaga tcgggtatta caatgaaaac catccgcata gtgcgttagg
      841 ttatctctca ccaagggaat atcgacgtca gcaagtaacg tcaccttaga tgcagtagtt
      901 gtcaggaaat agtgactcaa gggcaggctt accatataca ctcttagtgg ttcgactggg
      961 tattagaata gctattaaca gattagcact gaacactgcg taatagtggt tgcacaaaca
     1021 aaatgttatg ttataacatt gctgtattga catatatttt cgaatgtata tgaacgtaat
     1081 cagaactgtc atttgtacat taatcatact tccggtgggg ctacagacag cgacaagtca
     1141 ttcttctatg gctaaagata caatcaccgt tgtcgcgaca ggaaatcaga acacggtatt
     1201 tgaaacgccg tcgatggtca gtgtcatcac gaatgacacg ccatggagtc agaatgcggt
     1261 cacatcagcc ggtatgctga aaggtgttgc gggtctcagc cagactggtg caggacggac
     1321 caatgggcag acctttaatc tacgcggcta tgacaaaagc ggggtacttg ttcttgttga
     1381 cggcgtccgt caactcagtg acatggcaaa aagcagtggc acttatctgg agccggcact
     1441 catcaaacgc atcgaagttg tccgcggacc aaactccagt ctgtacggca gtggcgggct
     1501 ggggggggtg gtggacttca gaactgccga tgctgcagat tttcttcccc ccggagagac
     1561 aaacggtgta agtctgtggg gaaatgtcgc cagtggtgac cacagcacag gctcggggct
     1621 cacctggttc ggtaaaaccg gaaaaacaga tgcgcttctt tctgtcatta tgcgtaaaag
     1681 aggaaatatc tatcagagtg atggtgagcg tgcacctaac aaggaaaaac ctgcagccct
     1741 gtttgcgaaa ggctctgtca gtataacaga cagtaacaaa gcaggtgcca gcttgcgtct
     1801 ctaccagaat aacaccactg aaccgggcaa tcccactctg acacatggtg acaacggcct
     1861 gcgtgacaga aaaacagcac aaaatgacgt gcagttctgg taccagtacg cccctgcgga
     1921 taacagcctc atcaatgtaa agtcaacgtt atatctcagt gatatcacta tcaagacaaa
     1981 cggtcacaac aaaacagcag aatggcgaaa caacagaacc tccggtgtta atgttgtcaa
     2041 caggagccat actctgattt ttccgggagc ccatcagtta agttatggcg ctgaatatta
     2101 ccgtcagcag cagaagccgg aaggctctgc cacactatat ccggaaggaa acattgactt
     2161 tacatcgttg tatttccagg atgaaatgac aatgaaaagc aacccggtta acattatcgt
     2221 cggttcccgt tatgaccggt acaagagctt caatccccga gccggagaac tgaaagccga
     2281 acgcctgtcc cccagggcgg cgatttcagt ctcaccgaca gactggctga tgatgtacgg
     2341 ctccatatcc tctgcattcc gtgcccccac aatggcagaa atgtacaggg atgatgtaca
     2401 tttttaccgc aagggtaaac ccaattactg ggtacctaac cttaatctga aaccagaaaa
     2461 taacatcacc cgtgagattg gcgcaggtat tcaactggac ggcctgctta cagacaatga
     2521 tcggctgcag ttgaaaggcg gatatttcgg aacggatgcc agaaactata ttgccacgcg
     2581 cgtggatatg aaacggatgc gttcttattc ttataatgta tcccgggccc gtatctgggg
     2641 atgggatatg cagggtaatt accagtctga ttattttgac tggatgcttt cttataaccg
     2701 cacggaaagt atggatgcca gtagcaggga atggctgggc tccggcaatc ctgacacact
     2761 catcagtgac atcagtatac ctgttggtca tggaggagtt tctgccggat ggcgcgctga
     2821 actttcggca gcagccacgc atgtgaaaaa aggcgatccc catcaggctg gctatgccgt
     2881 acattccttt tcactgtctt ataagcctgt aagtgttaaa ggctttgagg catcagtgac
     2941 tctggataat gccttcaaca agcttgccat gaatggcaaa ggtgtgccgc tttcaggcag
     3001 aactgtcagt ctttataccc gttatcagtg gtaacctcgc aggaaataaa tcatgatgaa
     3061 aaatacaggc tatatcttag ctctttgtct gacagcatcg gagcatgccc tggcccacga
     3121 tgtctggatt accggtaaac aggcagagaa caacgttacc gcagagattg gttatggtca
     3181 taatttcccc tcaaagggca caattcctga cagaaggaat ttctttgaaa atccccggct
     3241 ttataacggg aaagagataa taacgctgaa gccaacgtcc acggattatg tctataaaac
     3301 tgagtctgca ggcaacggta atagctacgt tctgtcaacg aaaatgaaac cgggatactg
     3361 gtcgagaacc tcgtcaggat ggaagcctgt cagtcgggag ggcagaaatg atgtggcata
     3421 ctgtgagttt gtgactaaat atgcaaaatc ttttattcct ggtgaacagc agatgccagc
     3481 acaactctat cagtctccaa caggggatga gcttgaaatc attccattat ccgatatcag
     3541 tcgtttcggt gaagatgtga agctgaaagt tctgtataaa acatccccgc ttgccggagc
     3601 tactctggag cttgactctg tcagttatct gaaatcatcc cgtcataccc atgcagctga
     3661 gcataaacat tcggctcata aagcagaact tacctttgta agtaatgagg acggtatcat
     3721 cactgtacct tcccttcatg tcgggcaatg gctggctaaa gtcaaaaata agaaagtatt
     3781 tccggacaag agtctgtgtg atgaaactgt cgatgtggca accttaagct tctcccgaaa
     3841 ttaataatac tattcctgcc ctgatttatg ggcagggtat tatcaatacg agaatagcct
     3901 accgacatcc gtgtaaaaaa ccacataatg atttttcata tccatttcca gatatactta
     3961 tgtaaagatc acctccaaaa aagcgtaata gtaataaaaa tccctgaagc accctgttca
     4021 aaccagaagg aatacataac aagacaataa aatgtgaaat ccatcacagc aaagaactct
     4081 ttgtttataa tataatgaaa a
//
LOCUS       DAZKHK010000253         4069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKHK010000253 DAZKHK010000000
VERSION     DAZKHK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.255"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            231..521
                     /locus_tag="ACHOS5_005082"
     CDS             231..521
                     /locus_tag="ACHOS5_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9779739.1"
                     /translation="MSTTASIRKAPRENQINIRATDEERAVIDYAASLVNKNRTDFII
                     ELAYQEAKNIILDQRLFVLDDERYHSFIRQLEAPVQNAEGRERLMAVKPEWK"
     gene            512..994
                     /locus_tag="ACHOS5_005083"
     CDS             512..994
                     /locus_tag="ACHOS5_005083"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9779740.1"
                     /translation="MEINVTAPALLTDEHILLPFDCGNEVLSNWLRSRAMKNQMLNAS
                     RTFVICLEGTLRVVGYYSLATGSVTHAGLGRSLRHNMPNPVPVVLLGRLAVDVCTQGY
                     GFGKWLLSDAIHRVASLAEQVGIKAVMVHAIDDDARGFYERFGFVQSVVASDTLFYKI
                     "
     gene            1278..1535
                     /locus_tag="ACHOS5_005084"
     CDS             1278..1535
                     /locus_tag="ACHOS5_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP9779741.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPARERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            1768..1842
                     /gene="tap"
                     /locus_tag="ACHOS5_005085"
     CDS             1768..1842
                     /gene="tap"
                     /locus_tag="ACHOS5_005085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP9779742.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGRCD"
     gene            1835..2692
                     /gene="repA"
                     /locus_tag="ACHOS5_005086"
     CDS             1835..2692
                     /gene="repA"
                     /locus_tag="ACHOS5_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP9779743.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRALDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTLALFAALDV
                     SEDAVAAARRSRVEWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            3605..3874
                     /gene="yacA"
                     /locus_tag="ACHOS5_005087"
     CDS             3605..3874
                     /gene="yacA"
                     /locus_tag="ACHOS5_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFP9779744.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            3874..>4069
                     /gene="yacB"
                     /locus_tag="ACHOS5_005088"
     CDS             3874..>4069
                     /gene="yacB"
                     /locus_tag="ACHOS5_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFP9779745.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPH"
BASE COUNT         1050 a          972 c         1047 g         1000 t
ORIGIN      
        1 cacacaggtt aaggcatttc ctgtttttca gccatttcgc aacaaaaatc acattaaaca
       61 acagtaatca gacatgattt gtgcgccaac acgtatcatc gttacaactt tcaggtatct
      121 attattgttt ttattaggga gcactttccc cgtgagtgat tttgtatagc gaacagttac
      181 tatttgcggt aatatttatt gtatagcagt ttgttaccaa tgggggaatt atgtccacta
      241 cagcaagcat ccgtaaagca ccgcgggaaa atcagattaa catccgggca acggatgagg
      301 aacgagccgt tattgactat gctgcgagtc tggtcaataa gaaccgtacc gatttcatca
      361 ttgaactggc ttaccaggaa gctaaaaaca tcatcctgga ccaacgttta tttgtgctgg
      421 atgatgaacg ttatcattca tttattaggc aactggaagc tcctgtgcag aatgcagaag
      481 ggcgtgaacg tctgatggcg gtaaagcctg aatggaaata aatgttaccg cgccggcatt
      541 gctgacagat gagcatatac ttctgccatt tgactgtggg aatgaggtgt taagtaactg
      601 gttgcgtagt cgggctatga aaaaccagat gctcaatgct tctcgcacgt tcgttatttg
      661 cctggagggt actttacgtg ttgtagggta ttactcatta gctactggtt ctgtcacaca
      721 tgccgggctt gggcgcagtt tgcggcataa tatgcccaat cctgtacctg ttgttctttt
      781 agggcgcctg gctgtcgatg tttgtaccca gggatatggt ttcggtaaat ggctgttgag
      841 tgatgccatt catcgggttg ccagtctggc tgaacaagtt ggcattaagg ctgttatggt
      901 tcatgcaatt gatgatgacg ccaggggttt ttatgagcgt tttggctttg ttcagtcagt
      961 tgtggcgtct gataccttgt tttataaaat ttgatgttac gcaattttat taggcgtagc
     1021 tttataatga tgtaaatcga tagtccctcg gactggcgtt cacatttcag aaatcatcgg
     1081 tcccgcccgg ggccggcaca caggttaagg catttcctgt tttcagccat ttcgcaacaa
     1141 aattcacatt aaacaacagt aattacagtt gatttgtgcg ccaacacaga tcttcgtcac
     1201 aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga tccctgtctg
     1261 agaagagagg aggctagatg tcgcagacag taaatgcaga gacttcctca tcgggaaaaa
     1321 agcgacctta cagaaggggg aatcctgttc ctgcgagaga acgacaaaaa gcgtctctcg
     1381 caagaaggag tgccactcat aaagcgtttc atgccgtcat tcagcttcgc ctgaaagaaa
     1441 agttaagtga gcttgctgat gaagacggga tcactcaggc acagatgctt gagtggctga
     1501 tagagtcaga ggttaagcgc agaaaatctt tgtgaatatt tgcgtttctt gctgtatcag
     1561 tgatgagatg ttagattgct gatcgtttta aggaattttg tggctggcca cgccgtaagg
     1621 tggcagggaa ctggttctga tgtggattta caggagccag aaaagcgaaa accccgataa
     1681 tctttctatg tttggcgacg aagaagatta ccggggctaa caagaaaccg catagaagct
     1741 gttgctctat gcggggagta tagttatatg cccggaaaag ttcaagactt ctttctgtgc
     1801 tcactccttc tgtgcaacat aagtgcagga aggtgtgact gatcttcaac aaacgtatta
     1861 ccgccaggta aagaacccga atccggtatt cacaccccgt gaaggtgccg ggacgctgaa
     1921 gttctgcgaa aaactgatgg aaaaggcggt gggcttcact tcccgttttg atttcgccat
     1981 tcatgtagca catgcccgtt cgaggggcct gcgtcggcgc atgccaccgg tactacgtcg
     2041 acgggctctt gatgcgttgc tgcaggggct gtgtttccac tatgacccgc tggccaaccg
     2101 cgtccagtgc tccatcacca cactggccat tgagtgcgga ctggcgacgg agtctgctgc
     2161 cggaaaactc tccatcaccc gtgccacccg tgccctgacg ttcctgtcag agctggggtt
     2221 gattacctac cagacggaat atgacccgct tatcgggtgc tacattccga ccgacatcac
     2281 gttcacactg gctctgtttg ctgcccttga tgtgtctgag gatgcagtgg cagctgcgcg
     2341 ccgcagtcgt gttgaatggg aaaacaaaca gcgcaaaaag caggggctgg ataccctggg
     2401 tatggatgag ctgatagcga aagcctggcg ttttgtgcgt gagcgtttcc gcagttacca
     2461 gaccgagctt aagtcccggg gaataaaacg tgcccgtgcg cgtcgtgatg cgaacaggga
     2521 acgtcaggat atcgtcaccc tggtgaaacg gcagctgacg cgtgaaatcg cggaagggcg
     2581 cttcagagga agcctggagg cggtgaagcg cgaaatcgac cgtcgcgtga aggaacgcat
     2641 gataatgtcg cggaacaaca actacacacg gttggccaca gcctcaccct gagtttgacc
     2701 tcatctgaat aatccgactc gcgccggagg cattcgcacg cctgaagccc gacagcgcac
     2761 aaaaaatcag caccacatac aaaaaacaat ctcatcatcc agtttctggt gcatccggtc
     2821 cccctgtttt tgatacaaaa cacgccttac agacggggaa ttttgcttat ccacattaaa
     2881 ctgcaaggga cttccccata aggttacaac cgttcatgtc ataaagcgcc agccgccagt
     2941 cttacagggt gcaatgtatc ttttaaacac ctgctcatat ctcctttaaa ctacttaatt
     3001 acattcattt aaaaagaaaa cctgttcact gcctgtcctg tggacagaca gatatgcacc
     3061 tcccaccgca agcggcgggc cccaactgga gccactttag ttacaacaca caaaaacaac
     3121 caacgaaaaa accccggtcc agcgcagaac tgaaaccaca aagccctccc tcataactga
     3181 aaagcgccgc cgcccccgcc cgaagggcgg gaacagagtc gcttttaatt atgaatgttg
     3241 taactaagca acaccatcgc tgtcagtctt ctggctggac gtactgaata cacgctcgta
     3301 agcggccctg acggcccgct aacgcggaga tacgccccga cttcgggtaa accctcgtcg
     3361 ggaccactcc gaccgcgcac aaaagctctc tcatggctga aagcgggtat agcttagcag
     3421 ggtgggaatg ggataggcga aatctatcaa tcagtaccgg cttacgccgg gcttcggcgg
     3481 ttttactccg gtatcatatg aaacaatgga gtgccgcctt ccatgccgct ggcgcggcat
     3541 atcctagtga cgatatctga atcgttatat actgtgtata tacgtagtaa tgacgaggtg
     3601 ataaatggca caggttaata tgagtttaag aatcgacgct gaactgaagg atgcttttat
     3661 ggccgctgca aaaagcatgg accgtaatgg ctctcagtta atccgggatt ttatgcgcca
     3721 gaccgttgaa cggcagcata atacctggtt ccgcgaccag gttgcggcag gacgtcagca
     3781 actcgagcac ggcgatgtgc ttccccatga catggtcgaa tcctctgctg cagcatggcg
     3841 ggatgaaatg agcaggaagg ttgccggtaa atgatggaga tattctggac catgctggcc
     3901 agccaggaca ggaagcgcat tcgtgagtac attgcggagc agaacctgat ggctgccatt
     3961 gaactggacg aacggattgg ttattcagcc agcagcctgg ccgggcagcc gtataaaggc
     4021 cgcaacggtc gtgtggaagg tacccgggaa ctggttattc acccgcatt
//
LOCUS       DAZKHK010000254         4062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKHK010000254 DAZKHK010000000
VERSION     DAZKHK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.256"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(70..1047)
                     /gene="wzzB"
                     /locus_tag="ACHOS5_005089"
     CDS             complement(70..1047)
                     /gene="wzzB"
                     /locus_tag="ACHOS5_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP9779746.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEQDLKDNIALRMKNLRDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(1194..2360)
                     /gene="ugd"
                     /locus_tag="ACHOS5_005090"
     CDS             complement(1194..2360)
                     /gene="ugd"
                     /locus_tag="ACHOS5_005090"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP9779747.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLRIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(2609..4015)
                     /gene="gndA"
                     /locus_tag="ACHOS5_005091"
     CDS             complement(2609..4015)
                     /gene="gndA"
                     /locus_tag="ACHOS5_005091"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9779748.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         1068 a          981 c          905 g         1108 t
ORIGIN      
        1 atcgcatccg gcaaaaaaaa cgggcatggt gtcaccaccc tgcccgtttc tcttaaatgc
       61 acaataatat tatttcgcgt tgtaattacg cagcgcgtta cgcccaagca caatccccgc
      121 gccaaccatg ccgcccagca gcacagccag aatcaaggta attgctttct tcgggctatc
      181 gcgacgaata ggtaacgtcg gtttcattac atagcggtaa gcatgaatat caagtttatc
      241 aacgtcaaga ttgtcgatat ccagcaggtt ttgacgagtc tgatagtagt ttgatgagaa
      301 caccaacgga cgggtcgctt cgtgcttaat catcgactcc agcgcttcgc tccccaaaag
      361 gaacatagta tcctgggtta cgtcctgtgt ctgttgaatc tgcggctttg tcacctgcgc
      421 ctgattcgca tactgcaatg cttcctgaat ctgacggata cgcagatctt tctgttcttg
      481 agcgaccact tcctgcgtct tcagcgaatc ccgcaagttt ttcattcgca gcgcgatgtt
      541 gtctttcagg tcttgttcca gttcttcatt tacctggtcg tcaacttgct ggatatactt
      601 cgccagctgt ttctgcgcac cttcaggtgt ttcacccaca taagacaccg ctaaaggtaa
      661 tgactgattt ttaacggtag gctcaatggt cagtttttca ggttcttcct gattatccag
      721 cgtttctgct aatgcagaaa atgtcgtgct gaaacgacca attaacatcg actggatctc
      781 agacactttc ggcgctgccg aaccataaat cacatttatg gcattgttat aaccagcgat
      841 ttgccccaca tcaggttgag taacgatagc tgtggaagtc cacttttctt tggcaacggc
      901 cagataccct atagccaggg caatagcaac aatgacagaa atgatgatag tcatcttgcc
      961 gcgccacaac tgcaccagta aatcaatcaa atcaatctgt tccgggtcat ggttttgccc
     1021 agaaacatta ttattttcta ctctcatcat taccctaacg gacgaaataa ttttaagtcg
     1081 ccacagttta gcgataatcg ccatgaatgc tataggataa atgataaaaa gaatgaatta
     1141 ttacaaaggg aacataagag aatcagggcg aaaatcgccc tgataacagg atgttagtca
     1201 ctgccaaaga gatcgcgggt gtagactttg tctgccacat ccttaagctc ttctgccata
     1261 cggttggaaa taatgacgtc ggcttgttgt ttgaaggtgg cgagatctcg ttccaggcga
     1321 gagttgaaga atgagtcttc tttcatcact ggctcgtata tgatcacttc aacgcctttc
     1381 gccttgatac gtttcataat cccctgaatg gaagacgcgc ggaagttatc tgacccgctc
     1441 ttcataatca gacgataaat acccaccact tgcggcttgc gtgacaaaat ggcatcggca
     1501 ataaagtctt tacgcgtgcg gttagcatcg acaattgccg aaatcaggtt attcggcaca
     1561 gactggtagt tcgccagtaa ctgcttggta tctttaggca gacaataacc accataacca
     1621 aacgacggat tgttgtaatg gttgccaata cgcggatcga gacaaacgcc ttcgataatc
     1681 tggcgagtat tcagacctaa actttctgca tagctatcca gctcattaaa gtacgctacg
     1741 cgcatcgcca gataggtatt agcaaaaagt ttaatcgctt ctgcttcagt ggagtcggta
     1801 aacagggtcg ggatattttg cttaatcgcg ccttcctgta acaacgcagc aaaacgttct
     1861 gcgcgttctg aacgctcacc aatgacaata cgtgacggat ggagattatc gtaaagagct
     1921 ttaccttcac ggagaaattc cggagagaaa ataaggttat cgatacgcag acgttcttta
     1981 attgatttgg tgaaacctac cggaattgta gatttaatca ccattaccgc gttgggatta
     2041 atttctgtca catcacggat gaccgcttcc acgcttgagg tattaaaata atttgttttc
     2101 gggtcataat cggtaggcgt ggcaataata acgtaatcgg catttttata cgcgtcatac
     2161 ttatctgtcg tagcacggaa attgagatct ttagtcgcca gatactcttc aatctcctta
     2221 tcaacaatcg gtgactgctt cttgttaagc atgtccactt tggcctgaac gatatccagt
     2281 gcaactactt cgtggttttg cgcaatcaga ataccatttg aaagaccaac ataacctgtt
     2341 cctgaaattg ttattttcat tagctctgac ttcttccggt taaacattta gagtggtcat
     2401 taattcacca ccacaacttc atctactgcg ggatttttaa cgctgatgta gatgaactgt
     2461 caaggcagcg atcctgctgt gcggcgctgt attatatcgc gtttttaact ataaattata
     2521 aaaaaaggcc ataacctgcc gctttgtata ataaaaaagc ccggagggtt tctccgggcc
     2581 ttgcattgat taattgattt aaatcagatt aatccagcca ttcggtatgg aacacacctt
     2641 ctttatcaat gcgcttataa gtatgcgcac caaaatagtc acgctgtgcc tggatcaggt
     2701 tcgcaggcag aacagcggca cggtagctgt cataataggc aaccgcagcg gcgaaggtcg
     2761 gaaccgggat accgttctgt accgcataag caacgacatc acgcagcgcc tgctggtagt
     2821 catcggcaat ttgcttgaag tacggagcca gcagcaggtt agcgatctgc ggattttcgg
     2881 cgtatgcatc ggtgattttc tgcaggaact gcgcacggat gatgcagcca gcacggaaaa
     2941 tcttcgcgat ttcgccgtag ttcagatccc agttgtactc ttcagacgca gcacgcagct
     3001 gagagaagcc ttgggcgtaa gaaacgattt tgcccagata cagagcacga cgaacttttt
     3061 cgatgaactc agccttgtcg ccagctggct gcgcttgcgg gccagagaga actttagacg
     3121 cagcaacacg ctgatcttta agagaagaga tataacgagc aaatacagac tcggtaatca
     3181 gcgacagcgg ttcgccgaga tccagcgcac tctggctggt ccatttaccg gtacctttat
     3241 ttgctgcttc atccagaatc acatcaacca ggtaattacc gtcttcatct tttttggtga
     3301 agatatcttt ggtgatgtcg atcaggtagc tgctcagttc accgttattc cactcggtaa
     3361 acgtctgcgc cagttcttcg ttggaaaggt tcagaccacc tttcagcaga gaataggctt
     3421 cagcaatcag ctgcatatcg ccgtattcga taccgttgtg aaccatcttc acatagtgac
     3481 ctgcaccatc ggcaccaata taggtaacgc atggctcacc gtcttcagcc actgcggcga
     3541 ttttggtcag gatcggtgca accagttcat aggcttcttt ctgcccacca ggcataatgg
     3601 aaggaccttt cagcgcgcct tcttcaccac cggagacacc ggtaccaatg aagttaaagc
     3661 cttcggcaga aagctcacgg ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat
     3721 caatgatgat gtcaccttta tcgagatatg gtttgaggga atcaatagca gcatccgtgc
     3781 ctgcacctgc tttcaccatt aacaggatgc gacgaggtgt ttccagagat tcaacaaact
     3841 ctttcaccgt atagtaagga accagtttct tgcctggatt ttcggcaatc acttcttccg
     3901 tcttttcacg ggaacggttg aaaatagaga cggtataacc acggctttcg atgttgagcg
     3961 caaggttgcg ccccattact gccataccga cgacgccgat ctgttgcttt gacattgttt
     4021 actcctgtca gggggtggtt accgcaaagg ttcggtttac at
//
LOCUS       DAZKHK010000255         4011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKHK010000255 DAZKHK010000000
VERSION     DAZKHK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.257"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..254)
                     /gene="yfdP"
                     /locus_tag="ACHOS5_005092"
     CDS             complement(<1..254)
                     /gene="yfdP"
                     /locus_tag="ACHOS5_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP9779749.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETP"
     gene            complement(269..580)
                     /locus_tag="ACHOS5_005093"
                     /pseudo
     CDS             complement(269..580)
                     /locus_tag="ACHOS5_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            725..1240
                     /locus_tag="ACHOS5_005094"
     CDS             725..1240
                     /locus_tag="ACHOS5_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779750.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            complement(1455..2129)
                     /gene="ymfK"
                     /locus_tag="ACHOS5_005095"
     CDS             complement(1455..2129)
                     /gene="ymfK"
                     /locus_tag="ACHOS5_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFP9779751.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            2220..2420
                     /gene="ymfT"
                     /locus_tag="ACHOS5_005096"
     CDS             2220..2420
                     /gene="ymfT"
                     /locus_tag="ACHOS5_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFP9779752.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            2464..3015
                     /gene="ymfL"
                     /locus_tag="ACHOS5_005097"
     CDS             2464..3015
                     /gene="ymfL"
                     /locus_tag="ACHOS5_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP9779753.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAADTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            3161..3340
                     /locus_tag="ACHOS5_005098"
     CDS             3161..3340
                     /locus_tag="ACHOS5_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9779754.1"
                     /translation="MRELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            3303..3848
                     /locus_tag="ACHOS5_005099"
     CDS             3303..3848
                     /locus_tag="ACHOS5_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFP9779755.1"
                     /translation="MILLSVKDHQHIDSAIPGRYTVQAPYKAGDGIGVLEMLSAIYDA
                     PASFLSSVSVHTQIMVGWTGAPKGAPVSDNAGYANPVQFTTSEIGVSGGEGNSLLSEA
                     AIMATVPALTRLNDEDLHKLSYVTTALRALRKVTLSDPQAHQVLVETLLNLQAERIRL
                     ADKANFHIHRLLNISGGHRHA"
BASE COUNT         1019 a          919 c          983 g         1090 t
ORIGIN      
        1 ggcgtttcgg tggtgtcttc ttcggaaatt ttcccgcggt tagttgggcg aacatatgag
       61 agtttgtgtt tgattttcac acggttctca tcaaacggtt cgatttccag attgagtgag
      121 accttccctt tggttttcgt gttcatcaca ccggaagcga cctcactgag aactgcgccg
      181 attttggttt caaatacgcc gccgtccagc tccccgataa atgcctgcac atcagtactg
      241 cgttcgctag ccattttgct gctcctcatc atatcgaccc tgcaaggtcg gttggtttct
      301 ccacaaaaca gagaagaaca cctgcggtgg cagccgcccg gatggattgg gttatgagcc
      361 cgtcgtccgg tgatgctctt ctctgttttg caaaaaagga cggtaccagc cggaagcaag
      421 ggtacaaact ggtaccgcca ggactacaca cagcataaac ttgtggtgcc gggtgcctcc
      481 cggtgcctgg cgaaggttgc acaccaggcg ggtgggtatc cacagaaggt cgactgtcag
      541 cctcaacctt aacccgcgtg cgctgagccg cattcaccac aacgctaagg attctctctg
      601 gttgaaaata cttagctgtt atgtgcctgc ttttagccac atcaggcgag gtggacctgg
      661 ttattcccca acaacaagga ttcggttaat ctggatatcc ccaacaacaa taagagtatt
      721 caatgtgatc gctgaattaa cggcagcaat gacggctatt cgtgaaacag cccagattgc
      781 aaaactaatg aacgaggcaa aaactcaagc tgaagtaaat gcggctattg gtgagctgaa
      841 ctcaaagctt gcgtctattc agcgcgaatg cgtgtctctc gttgaactgg tgggctctta
      901 tcaagaaata aatgcttctc tcaaagctaa aattgcagaa ttcgaaaact ttgaggctca
      961 gacggaaggc tatatcctta accaacttga gtcgggtact tttgtatact cgaaggaggt
     1021 aatcgtgaac ggtggcagca taaccatgca tctttgccca aaatgttttg gacaaaagat
     1081 agtatcgata cttcaaccat tcccggttag cgaagatgag ctttttcata aaagcaggtg
     1141 cctccactgt gaaaataagt ttctgatgaa taaaaatccg gattacgtat cgcctccatc
     1201 cattgaggag ttgtccagaa aacttaacgg caatctgtag attactactg ttgtggatat
     1261 ccagattgtt aaagagctaa gcgtcctgta gggcgctttt ttgttgctaa cgaatcatcc
     1321 tggacttcat atgccccagg cggctacttc gtgggcgtcc tgcctgtttg ttgtttctct
     1381 tgggtacatt atgtatctca taggtacatt gtcaagtata aaaaaacctg ccgaagcagg
     1441 ttcataaaca ttgattaggc tttgattttg tatcttcttg gttttcctga gaaaatcaca
     1501 gttccaatta tagagcaatt accgttgatc ttaatgtaag gctcaggcca gtttgggttt
     1561 aacgctttga gataacgctg tgtcccatct tctatcaacc ttttgaaggt ggtttcacct
     1621 gtatcgtgca tcaatgcaat aacgtcgtca ccgtggcagg caggtacttc aggatcgaca
     1681 aaaatcatgt ctcccgggcg gtactcatca atcattgaat cacctatcac ccgcaagata
     1741 taagtcattt ccccacaggg tacagggcag ggatacgttt ctgctgtgct caaatcaacc
     1801 tcagaatatc caacttcttt ccatgctccg gcctgtaccc atgatatgac agggactaat
     1861 gtgatttgtt tattagtgat tgaaacatca ggtttttttg tgatgttcgt tgtctggtgt
     1921 tcttgatcga gccatccgac aggcaggtcg aaacattttt cgatgtgtcg tgccatgctg
     1981 tcaccgatat ttttagtagc accatctccc ataaacctgc tggtctgggt tggctcgcga
     2041 tcaatcatag tggcaaagga agaattccct ccaacaccat ctctcagttt tctggcgtta
     2101 gaccgccgga tgtcatggat tgttttcata acgaaattaa aacccttgta ccgttaaggt
     2161 acaagtatct tgaaggttca tttcaatcat gtaatatgta caccggaggt acatattgta
     2221 tgaaagcgta ttgggactct ttaaccaaag aacagcaggg cgagttggcc ggaaaagttg
     2281 gctcaactcc tggctactta cggctggttt tcaatggtta taaaaaagcc agttttgtgc
     2341 tggcgaaaaa acttgagcaa tgcacgtcag gtgcaattac gaaatctgac ttaagaccgg
     2401 atatctatcc gaaagattaa cagaacacct tcaattttta accacagaac gatgaggcta
     2461 accgtgggta agtatcactg gaaagtagaa aaacagcctg agtggtacgt gaaagctgtc
     2521 agaaaaacta tcgcagcgtt gccgggtggt tacgctgaag cagctgactg gctggatgta
     2581 acagagaacg cattatttaa ccgccttcgt gccgatggcg atcagatttt cccgctggga
     2641 tgggcaatgg ttttacagcg cgcggctgac actcactaca ttgcggatgc tgtcgcacag
     2701 tctgctggtg gggtgttcgt atcgcttcct gaaattgagg aagtagagaa cgccgatata
     2761 aaccagcgcc tgctggaagt catcgaacag atcgggaatt actcaaagca gattcgttcg
     2821 gcaatcgaag atggggtcgt ggagccacac gagcagacag caattaatga tgagttgtat
     2881 ctgtcaattt cgaagctcca ggagcatgca gcactggtct acaaaatctt ttgcgctcca
     2941 gaaaagagtg acgcccgcga gtgtgcagct ccgggcgtcg tggcgttttg tgtctgtgga
     3001 gaaactaacg catgaacagt ttaacggcaa ataaccgttt gtcgcaacag ctggtggtca
     3061 gtgtcgctga acacctgttg ttacggcatg aatgcagatt accaaatcac ctggctgtaa
     3121 gtaaccacag agaactttac ctgactgtgg ggggcgagtt gtgcgggaac ttaaccgctg
     3181 gtttcgtgac ggaagagggc tttatgtcca tgttattcgt tgggaaccag aaacacagcg
     3241 cgttatctat cttcgcaaag actacccgca tgagtgcttt agtcctttgt ggaaattcag
     3301 gcgtgatttt gttgagtgtg aaggaccacc agcacattga ttctgccatt ccgggacgtt
     3361 acactgttca ggcaccttat aaagcgggtg acgggattgg cgtcctggaa atgttatcgg
     3421 cgatatatga cgcgccagcg tcttttttat cgtctgtgtc tgtgcacacc caaattatgg
     3481 tgggctggac gggggcaccg aaaggtgcgc cggtttccga taacgccggt tacgccaacc
     3541 ccgttcagtt caccaccagc gaaattggcg tttccggtgg tgaaggtaat tcactgttat
     3601 cggaggctgc catcatggct actgtcccag ccctcactcg tctgaatgat gaagacttac
     3661 ataaactcag ttatgtaaca actgcactac gtgctctgcg caaggtaact ctttcggatc
     3721 cgcaggcgca tcaggttttg gtagaaaccc ttcttaactt gcaggctgaa cgtattcgtc
     3781 tggcggataa ggctaatttt catattcacc gtctcctgaa tatcagcgga gggcatcgtc
     3841 atgcttaatc cgttgatcct caatatttgc cgtttgcttc agcgtaaaaa aacatcaatt
     3901 cctacagttg ggcagtggta caccacgcct gcagggcatg ttctacgtgt tagcctggtt
     3961 gaccgtgaat gtcagaaggt gatttgtgaa ccgctgggcc gtaattaccg c
//
LOCUS       DAZKHK010000256         4011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKHK010000256 DAZKHK010000000
VERSION     DAZKHK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.258"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            7..201
                     /locus_tag="ACHOS5_005100"
     CDS             7..201
                     /locus_tag="ACHOS5_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9779756.1"
                     /translation="MQGSGVTDGIGFTLTNNIDCESGIIFDNDEDKTDSAVLFPWIER
                     ACQDVRELCQFQRLQKTRTD"
     gene            283..1140
                     /locus_tag="ACHOS5_005101"
                     /pseudo
     CDS             283..1140
                     /locus_tag="ACHOS5_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(1261..1337)
                     /locus_tag="ACHOS5_005102"
     tRNA            complement(1261..1337)
                     /locus_tag="ACHOS5_005102"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1301..1303),aa:Asp,seq:gtc)
     gene            complement(1470..2201)
                     /gene="dnaQ"
                     /locus_tag="ACHOS5_005103"
     CDS             complement(1470..2201)
                     /gene="dnaQ"
                     /locus_tag="ACHOS5_005103"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP9779757.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2266..2733
                     /gene="rnhA"
                     /locus_tag="ACHOS5_005104"
     CDS             2266..2733
                     /gene="rnhA"
                     /locus_tag="ACHOS5_005104"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP9779758.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITKWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2730..3452)
                     /gene="yafS"
                     /locus_tag="ACHOS5_005105"
     CDS             complement(2730..3452)
                     /gene="yafS"
                     /locus_tag="ACHOS5_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9779759.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3486..>4011
                     /gene="gloB"
                     /locus_tag="ACHOS5_005106"
     CDS             3486..>4011
                     /gene="gloB"
                     /locus_tag="ACHOS5_005106"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9779760.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSVLPDDTL
                     VCCAHEYTLSNMKFA"
BASE COUNT         1079 a          978 c          979 g          975 t
ORIGIN      
        1 tttagtctgc aaggatcagg agttacagac ggcattggct ttacgcttac gaacaatatc
       61 gactgcgaat cgggaattat ctttgacaac gatgaggata aaacagattc ggcagtactg
      121 tttccctgga ttgaacgtgc atgtcaggac gttcgcgagc tgtgtcagtt tcaacgtctg
      181 caaaagaccc gtacagatta actccctgtc cgtactcatt acacacatac acgtgccagc
      241 ccccaccggc tggcgctttg ctttgtataa aggaaataaa taatgtcaga acctactctc
      301 accaaccccg aagtattaac cgatcatacc gatgtgattt gctccaccag tattgaacgc
      361 atcgtcaccg gacgtaatgc ggcgctgaca cagattgaag cattgctcca ccagattaac
      421 gatatttcga cacaaaccct cagtatcggc ggaaaaaccg ccctggactg ggtcatgaaa
      481 caggattttc gctgtggttg ctggcttatg gagaagcctg aaacagcgat gaaagccatt
      541 acccgcaatc tcgatcgcgg taaatggcgg gatttaatga aacgctcagg gatgctgtcg
      601 attatggacg ctcaggcccg taaccagtgg tacaacagcc tagaaaagga tgatattccg
      661 gtggtcagcg aagcgaatat tctgagcacc tttaagcaac tgcatttgaa caagggtgag
      721 gtgttttagc gtggcgtcat taacgttttc aaaggattaa gctggaattt taagacgaat
      781 tcgccctgcg ggtttggagc gaaaatcatt gtgacgggac tggtcaaatt cgaccggtgg
      841 ggattcgggc ttaaccatgg ctgacagcgt gaccggttag cagaccttga gcgaatgctg
      901 atgatccttg acgggaagcc tgttcccgac aaccgcgctg acgttacccg tcggctggga
      961 gaccatattc atgaaaaccg gcacagcaac cactatgagg atgagatgtt tgtgattaag
     1021 tattttcaga agggaactgc acaaatcgta ttcaagcgac ctgagctgat tgataagctg
     1081 aacaacatca tcgcccgaca ccaatccggg agcactgcca gcgagatgag aggtgagtga
     1141 gttgtctgaa gctatttcag gtgagccagt gcaagccgga cggcaatggc atactggatg
     1201 gcataccgaa aaaacaagca tataaaaggc catgctaatc aacagcatgg cctcgaatag
     1261 tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc
     1321 gactgaacta ccgctccgcg ttgtgttccg ttgggaacga ggcgaatatt acggattgcc
     1381 tcacccttcg tcaacggttt ttctcatctt ttgaatcgtt tgctgcaaaa atcgcccaag
     1441 tcgctatttt tagcgccttt tacaggtatt tatgcccgcc agaggcagct tccgcccttc
     1501 ttctgcacca gatcgagacg ggcttcatgt gctgcaagct cttcatctgt cgcaaaaaca
     1561 acgcgtaact tacttgcctg acgcacaatg cgctgaattg ttgtttcacc ttgttgctgt
     1621 tgtgtctctc cttccatcgc aaaagccatc gacgtttgac caccggtcat cgccagataa
     1681 acttccgcaa ggatctgggc atcgagtaat gccccatgca gcgttcgttt actgttatct
     1741 atttcgtagc gggcacataa cgcatcgagg ctgttgcgtt taccgggaaa cattttcctc
     1801 gccaccgcaa ggctatcggt gaccttacag aaggtattgg tcttcggaat atcgcgctta
     1861 agcaacgaaa actcgtagtc cataaagccg atatcaaacg ctgcgttatg gatcaccaac
     1921 tccgcgccgc ggatataatc catgaactca tcggctacct cggcaaacgt gggcttatcg
     1981 agcaaaaatt catcggcaat accatgtacg ccaaaggctt ccggatccac cagccgatcg
     2041 ggtttgagat aaacatggaa gttattgccc gtcaggcgac ggttcaccac ttcaacggca
     2101 ccaatctcaa tgatcttgtg gccttcatag tgcgcaccaa tctggttcat accggtggtt
     2161 tcggtatcga gaacgatctg gcgtgtaatt gcagtgctca tagcggtcat ttatgtcaga
     2221 cttgtcgttt tacagttcga ttcaattaca ggaagtctac cagagatgct taaacaggta
     2281 gaaattttca ccgatggttc gtgtctgggt aatccaggac ctgggggtta cggcgctatt
     2341 ttacgctatc gcggacgcga gaaaaccttt agcgctggct acacccgcac caccaacaac
     2401 cgtatggagt tgatggccgc tattgtcgcg ctggaggcgt taaaagaaca ttgcgaagtc
     2461 attttgagta ccgacagcca gtatgtccgc cagggtatca ccaagtggat ccataactgg
     2521 aaaaaacgtg gctggaaaac agcagacaaa aaaccagtaa aaaatgtcga tctctggcaa
     2581 cgtcttgatg ctgctctggg gcagcatcaa atcaaatggg aatgggttaa aggccatgcc
     2641 ggacacccgg aaaacgaacg ctgtgatgaa ctggctcgtg ctgcggcaat gaatcccaca
     2701 ctggaagata caggctatca agtcgaagtt taagcctgtg gtttacgaca ttgccgggtg
     2761 gcaccaaccg cctggcgaat tcgcggttta ttcttgctct gtttcatcgg atttagcgtt
     2821 aaaggaatag tccgtttccg ggcaacaata agttgtaaac aaccaagcgc aggaatatgc
     2881 gcattcaata gttttcctcc gtgtttgttc cacgggagaa cgtggaaacg gctggcgtgt
     2941 agcacttcaa aattcaacaa agagagccag tccaactgcc gcatcagagt aaacatccgg
     3001 ctgttatagg gcgaggtttt gcgcaatacc ggcacaagtt tgcgtaatcc cattaaactg
     3061 atgggattga agccactaat caccagccag ccatcatcaa tcagtacccg atcggcttca
     3121 cgcaataaac gatgcggatc agtgcaccac ggcaatgtat gtgccagtag acaaacatca
     3181 acggatttat cggcaaaagg aagatgaagt ggatccgcct gcacctggac gggcattcct
     3241 tgcgcagaaa cattcacttg atgagaaacc gcgcacgctt cgcaattgat ttctgcgctt
     3301 aaattgccaa tcttaagcag atgaaaacca tacattttag tgaaccacgg gttgagctga
     3361 cgctccagcg ccttgcgata aagctctccc cagggcaaat cgccccagca atcaggagcc
     3421 acgacagttt gagggactct tgccggtttc atcacaacct tccgtttcac actgagaggt
     3481 aatctatgaa tcttaacagt attcccgcct ttgatgacaa ttacatctgg gttttgaatg
     3541 atgaagcagg tcgctgcctg attgtcgatc ccggggacgc agagccagta ttaaacgcca
     3601 tttccgccaa taactggcaa ccggaggcca tatttctcac ccaccatcac cacgatcacg
     3661 ttggcggcgt aaaagaactg gtggaaaagt ttccgcaaat tgtggtgtat ggaccacaag
     3721 agacacaaga taagggaaca acacaggtag tcaaagatgg cgaaactgcc ttcgttttgg
     3781 ggcatgaatt tagtgtaatt gccacaccgg gtcacacttt aggacatatc tgttacttca
     3841 gtaaacctta tctattttgc ggcgacacgc tgttttctgg tgggtgtggt cggttgtttg
     3901 aagggacacc atcacaaatg tatcaatcac ttaaaaagtt aagtgtgtta cctgacgaca
     3961 cattggtatg ctgtgctcat gaatatacct tatcaaatat gaagtttgct t
//
LOCUS       DAZKHK010000257         3913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKHK010000257 DAZKHK010000000
VERSION     DAZKHK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.259"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..147
                     /locus_tag="ACHOS5_005107"
     CDS             <1..147
                     /locus_tag="ACHOS5_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9779761.1"
                     /translation="DWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQ
                     KGAA"
     gene            144..1214
                     /locus_tag="ACHOS5_005108"
     CDS             144..1214
                     /locus_tag="ACHOS5_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9779762.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAARLEALWKHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFCDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAFPDTTILEIRPR
                     KRLKHTGEEGKSGGLLSFTSAHTDIWRQQGYEDTMLTMEHIRNPLAARQALTRSEAVL
                     QKSQDITEEADSALKNAMARIK"
     gene            1347..1889
                     /locus_tag="ACHOS5_005109"
     CDS             1347..1889
                     /locus_tag="ACHOS5_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779763.1"
                     /translation="MKFIVKALIYLAVMLSFTANAAEYKEYPQGEITYYKYLPRNGWT
                     LPAGYTVEQFSSAIYKGQIRNNFPWTNQFIVRGNGVLFLANKVNKTWHVLPVDYQRLN
                     FGRLTTHYQHVNKGDGCYFYILDGHGSDAKPILKIEENCVDMKMYRKMVAEKKEREQK
                     ANQWLQQQILAEHCRRYGNC"
     gene            2244..2732
                     /locus_tag="ACHOS5_005110"
                     /pseudo
     CDS             2244..2732
                     /locus_tag="ACHOS5_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3100..>3913
                     /locus_tag="ACHOS5_005111"
     CDS             3100..>3913
                     /locus_tag="ACHOS5_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9779764.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTHVRDPGRRRYTKHMLRLRRAGEINGQHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILMPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLES"
BASE COUNT         1029 a          934 c         1006 g          944 t
ORIGIN      
        1 gactggtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc agcatgagta cagaaatgaa aacggggctg gtgctgtccg
      181 gcggcggggc ggtgggcgct tatcaggcag gtgtggttaa ggcactggca gagtgtggca
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcgg
      301 cctctcccga tctgtcagaa gcagccgcac gcctggaggc gctctggaag catctgggga
      361 ataatcaggt gctgtcggta aaccgatcgg tttacttttc attgctgaaa aaattgttcc
      421 aggcaatgaa cctctgccag atccccggac gtgcaggcgc actgcttacg acacttcttc
      481 gccatatatc gataatcaac ggcttcgaca atccgatggc tcagccgctg ttgtcagatg
      541 agcctctgac agcgctgatg gatcattatc tcgatactga tgctctggca gacgggctac
      601 cgctgtatgt gtcgctgtac cccacagaag ggggcatgca ggatattatt gactgcattc
      661 gtgctgaact gggtgccgga accacgaaaa acgccgtttt tcagcatatc cagagtctgc
      721 cccgcggaca gcagaaagag gctctgcttg cgtcagccgc actgcccctg ttgttccgtc
      781 cccgtgaggt tcaggggaca atgttctgtg atggtggtat gggaggatgg cgaaatatgc
      841 agggaaatac ccctgtaaca cctctggtcg atgccggatg caatatggtg attgtgacgc
      901 atctgagtga cggttcttta tgggatcgcc gggcttttcc ggacaccaca atccttgaaa
      961 tccgtccccg gaaaaggctg aaacacacag gggaagaagg caaaagtggc ggtctgctta
     1021 gttttacatc ggcacatacc gacatctggc gtcagcaggg ctatgaagac acgatgctga
     1081 cgatggagca tatccggaac cccctggcgg cgcgtcaggc actgacccgg tcagaggcgg
     1141 tattgcagaa aagccaggat ataacggaag aggcagattc ggcactgaaa aacgcgatgg
     1201 cccggattaa ataaagacgc cccaaagaaa cgccaccgcc tgtggtggtg tttttcctgg
     1261 ctggtgttat tgaaaatatt ttcagatata tgttacccgg gtgagattta atattgacgc
     1321 atttattact gtcataagga gttatcatga aattcatcgt aaaagcactt atttatcttg
     1381 ctgtcatgtt atcattcaca gccaacgctg ctgaatataa agaatatcca caaggagaaa
     1441 ttacctatta caaatacctt cccaggaacg gctggacact gcctgcaggc tataccgtag
     1501 aacaattttc cagtgccatt tacaaagggc aaatacggaa taacttcccc tggacaaatc
     1561 agttcatcgt gcgtgggaac ggggtacttt tcctggccaa caaagtcaat aaaacctggc
     1621 atgttcttcc ggtggattat cagagattaa acttcgggag attgacgacc cattatcaac
     1681 atgtaaacaa aggcgacggt tgttactttt acatcctcga tggccatggt tcagatgcaa
     1741 aaccaatttt aaaaattgaa gaaaactgcg ttgacatgaa aatgtacagg aaaatggttg
     1801 cagagaaaaa ggaacgcgaa caaaaagcga accaatggct ccagcagcag attcttgcag
     1861 aacattgccg tcgctacggt aactgctaat aacaccggta agtaaaaaag agggttctgg
     1921 tttccagatt attttaaaag cattttatac catcgttatt ttctgtgctg ttggtatcag
     1981 catatttaca ctttcggcga gccggatatt cactattaca acccggctct gtattcatcc
     2041 ataatcaatc ctgatgaagt ccctgtacta atgcgcatgt ttatcaacaa ctcaatgata
     2101 ttgtctgcgg gcaaccgaag gtaatatccg gcagtatcca tccattagcg gtcggtaatt
     2161 ataccgctgt ggatttcttt cacagcgctg aattaatcgg tcgtattacc ttaaatcgca
     2221 gtgctgaata acttaagaag gaatttataa tgaaacgttt acatactctg atgctctttc
     2281 tggttttcct gtttattggt gtaagtcctg acacaaacgc cgccactgta aatcagacac
     2341 ttaaatgcga ctcaaaagcc cgggctggcc agcctggtgc atgtccaaca acgtacgagt
     2401 tatacgaagg tgacgctacc tacagagctg ctatcgacaa agcattaaac cctgttgggt
     2461 taaacggaat gtttggggaa gacggctata tggatggtcc tgatggtggt gcgtatcctg
     2521 taaacattaa cggaacgacc tgggtagaag gtggcggttg taaagcccac gcctgcggct
     2581 gggactatat cgtaacactc tataacccaa aaacgcataa agtcgttggt tactattaca
     2641 acatcgaccc tggttacctg atttggtttg gtgaaactgg cgttcacgaa ttcgcgtacc
     2701 tggtcaggga ctacgtaaac aaaacaaatt aaaggcgtaa gttatgtgat tcatctgcgc
     2761 gttattaata tcaaaagtaa atacggcccc agccatgatg gcagggcttt ttaatttcag
     2821 ggagcaacag atatgtcagg ttgtacttcg cgtttattcc ctgagggacc ttttgctgcc
     2881 aaatggcatc tgaatcgatc acagtctggc cggttattac ccgatcccga tgggattatt
     2941 gggtgatctc cttccataaa aatatgcagc tcgccagtga agggcttctc agacaataaa
     3001 cgtcattgat ttcgtccatc aggccgcgtc ttctccggga gacgcggctt ttttcattta
     3061 ttacaccagt aaatcttaac caccgataag gagcaaagta tgcgattagc cagtcgtttt
     3121 ggttatgctg caaaccagat acgccgtgac cgtccactga cacatgaaga gctgatgcac
     3181 catgtgccgg ggattttcgg agaagataaa cacacgtccc gaagccagaa ctatacgtac
     3241 atccccacca tcaccgtgct ggaaagtctg cagcgggaag gcttccagcc attcttcgcc
     3301 tgccagaccc atgtgcgcga cccgggccgt cggagataca ccaaacacat gctgcgtctg
     3361 cggcgggccg gagagataaa cggacaacat gtccctgaaa tcatcctgct gaactcccat
     3421 gacggtacct ccagctacca gatgctgccg ggttacttca ggttcgtctg ccagaacgga
     3481 tgcgtctgtg gtcagtctct gggggaagtg cgtgttccgc accggggaaa tgtggtggag
     3541 aaagttatcg aaggggctta cgaggtggtg ggggtttttg accggataga ggagaagcgt
     3601 gatgccatgc agtcgctggt cctgccgcca ccggcacgcc aggcgctggc acaggcggca
     3661 ctgacttacc gttatggtga cgaacatcag cccgtcacca ccgccgacat tctgatgcca
     3721 cgacgccggg aggattacgg taaggacctg tggagtgctt atcagaccat tcaggagaat
     3781 atgctgaaag gcggaatttc aggtcgcagc gctaaaggaa aacgtatcca tacccgtgcc
     3841 attcacagca ttgacactga cattaagctc aaccgcgcat tgtgggtgat ggcggaaacc
     3901 ctgctggaga gcc
//
LOCUS       DAZKHK010000258         3886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKHK010000258 DAZKHK010000000
VERSION     DAZKHK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.260"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..525
                     /locus_tag="ACHOS5_005112"
     CDS             <1..525
                     /locus_tag="ACHOS5_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9779765.1"
                     /translation="LADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSAAVIKWYA
                     ERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNARDSAEVV
                     ETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDE
                     LIPGLLSEYIEQSG"
     gene            500..2425
                     /locus_tag="ACHOS5_005113"
     CDS             500..2425
                     /locus_tag="ACHOS5_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9779766.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2422..2628
                     /locus_tag="ACHOS5_005114"
     CDS             2422..2628
                     /locus_tag="ACHOS5_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP9779767.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2625..>3886
                     /locus_tag="ACHOS5_005115"
     CDS             2625..>3886
                     /locus_tag="ACHOS5_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9779768.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRDKLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIV"
BASE COUNT          907 a          984 c         1190 g          805 t
ORIGIN      
        1 ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga acagggaatg
       61 cccgttctgc gaggcggtgg caagggtaat gaggtgcttt atgactctgc cgccgtcata
      121 aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa
      181 gaactgcggc aggccagcga ggcagatctc cagccaggga ctattgagta cgaacgccat
      241 cgacttacgc gtgcgcaggc cgacgcacag gagctgaaaa atgccagaga ctccgctgaa
      301 gtggtggaaa ccgcattctg tactttcgtg ctgtcgcgga tcgcaggtga aattgccagt
      361 attctcgacg ggatccccct gtcggtgcag cggcgttttc cggaactgga aaaccgacat
      421 gttgatttcc tgaaacggga tatcatcaaa gccatgaaca aagcagccgc gctggatgaa
      481 ctgataccgg ggttgctgag tgaatatatc gaacagtcag gttaacaggc tgcggcattt
      541 tgtccgcgcc gggcttcgct cactgttcag gccggagcca cagaccgccg ttgaatgggc
      601 ggatgccaat tactatctcc cgaaagaatc cgcataccag gaagggcgct gggaaacact
      661 gccctttcag cgggccatca tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt
      721 ggtgaagtct gcccgtgttg gttattccaa aatgctgctg ggtgtttatg cctacttcat
      781 agagcataag cagcgcaaca cccttatctg gttgccgacg gatggtgatg ccgagaactt
      841 tatgaaaacc cacgttgagc cgaccattcg cgatattccg tcgctgctgg cgctggctcc
      901 gtggtatggc aaaaagcacc gggataacac gctcaccatg aagcgttttt ccaatggtcg
      961 tggcttctgg tgcctgggcg gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt
     1021 ggcgggttat gatgaacttg ctgcttttga tgatgatatt gaacaggaag gctctccgac
     1081 gttcctgggt gacaagcgta ttgaaggctc ggtctggcca aagtccatcc gtggctccac
     1141 gcccaaagtg agaggcacct gccagattga gcgtgcagcc agtgaatccc cgcattttat
     1201 gcgttttcat gttgcctgcc cgcactgcgg ggaggagcag tatcttaaat ttggcgacaa
     1261 agagacgccg tttggcctca aatggacgcc ggatgacccc tccagcgtgt tttatctctg
     1321 cgagcataat gcctgcgtca tccgccagca ggagctggac tttactgatg cccgttatat
     1381 ctgcgaaaag accgggatct ggacccgaga tggcattctc tggttttcgt catccggtga
     1441 agagattgag ccacctgaca gtgtgacctt tcacatctgg acggcgtaca gcccgttcac
     1501 cacctgggtg cagattgtca aagactggat gaagacgaaa ggggatacgg gaaaacgtaa
     1561 aaccttcgta aacaccacgc tcggtgagac atgggaagcg aaaattggcg aacgtccgga
     1621 tgctgaggtg atggcggagc ggaaagagca ttattcagcg cccgttcctg accgtgtggc
     1681 ttacctgacc gccggtatcg actcccagct ggaccgctac gaaatgcgcg tatggggatg
     1741 ggggccgggt gaggaaagct ggctgattga ccggcagatt attatgggcc gccacgacga
     1801 tgaacagacg ctgctgcgtg tggatgaggc catcaataaa acctataccc gccggaatgg
     1861 tgcagaaatg tcggtatccc gtatctgctg ggatactggc gggattgacc cgaccattgt
     1921 gtatgaacgc tcgaaaaagc atgggctgtt ccgggtgatc cccattaaag gggcatccgt
     1981 ctacggaaag ccggtggcca gcatgccacg taagcgaaac aaaaacgggg tttaccttac
     2041 cgaaattggt acggataccg cgaaagagca gatttataac cgcttcacac tgacgccgga
     2101 aggggatgaa ccgcttcccg gtgccgttca cttcccgaat aacccggata tttttgatct
     2161 gaccgaagcg cagcagctga ctgctgaaga gcaggtcgaa aaatgggtgg atggcaggaa
     2221 aaaaatactg tgggacagca aaaagcgacg caatgaggcg ctcgactgct tcgtttatgc
     2281 gctggcggcg ctgcgcatca gtatttcccg ctggcagctg gatctcagtg cactgctggc
     2341 gagcctgcag gaagaggatg gtgcagcaac taacaagaaa acactggcag attacgcccg
     2401 tgccttatcc ggagaggatg aatgacgcga caggaagaac ttgccgctgc ccgtgcggca
     2461 ctgcatgacc tgatgacagg aaaacgggtg gcaacggtac agaaagacgg acggagagtg
     2521 gagtttacgg ccacttccgt gtctgacctg aaaaaataca ttgcggagct ggaagtgcag
     2581 accggcatga cacagcgacg cagggggcct gcaggatttt atgtatgaaa acgtccacca
     2641 ttcccaccct tctggggccg gacggcatga catcgctgcg tgaatatgcc ggttatcacg
     2701 gcggtggcag cggatttggt gggcagttgc gggcgtggaa cccaccgggt gaaagtgtgg
     2761 atgcagccct gctgcccaac tttacccgtg gcaatgcccg cgcagacgat ctagtacgca
     2821 ataacggcta tgccgccaac gccatccagc tgcatcagga tcatatcgtc gggtcttttt
     2881 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gacgcccgtg
     2941 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     3001 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     3061 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     3121 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     3181 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     3241 tcagcgagga cggctatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     3301 tacccggcgg gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac gggcagaccc
     3361 gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga
     3421 acacgcagct gcagagcgcc attgtgaagg cgatgtatgc cgccaccatt gagagtgagc
     3481 tggatacgca gtcagcgatg gattttattc tgggcgcgaa cagtcaggag cagcgggaca
     3541 agctgaccgg ctggattggt gaaattgccg cgtattacgc cgcagcgccg gtccggctgg
     3601 gaggcgcaaa agtaccgcac ctgatgccgg gtgactcact gaacctgcag acggctcagg
     3661 atacggataa cggctactcc gtgtttgagc agtcactgct gcggtatatc gctgccgggc
     3721 tgggtgtctc gtatgagcag ctttcccgga attacgccca gatgagctac tccacggcac
     3781 gggccagtgc gaacgagtcg tgggcgtact ttatggggcg gcgaaaattc gtcgcatccc
     3841 gtcaggcgag ccagatgttt ctgtgctggc tggaagaggc catcgt
//
LOCUS       DAZKHK010000259         3813 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKHK010000259 DAZKHK010000000
VERSION     DAZKHK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.261"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>3813
                     /locus_tag="ACHOS5_005116"
     CDS             <1..>3813
                     /locus_tag="ACHOS5_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFP9779769.1"
                     /translation="RFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQEL
                     TERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAP
                     ENAAGRQGEILEGETVRRGLPSPDEQNATALVREGLPAPDITHNVRMPQPDQLPRTVR
                     NSLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVATDREATVRGGEVRGKKIEDFG
                     EEIKGAAKHRYAQLAETLGKTLEDRDYVTQPLSKLFPKPDYAKLANEGADADTLAMIA
                     LYRSDIPAKTKHNMAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQ
                     LLRTLPPSQMDKASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDE
                     LLTKAKAWFAEQEEKSQAKGDEKTTPSPDDKIRFDVYRSTRSGDIFIAYGKNKMRVRG
                     GFKSASDARKYIDSHRDELVRHVKEMREVSREEQRNATNRDRTGPERRKGDVSPEQFS
                     DAFGFRGVQFGNYVEGPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARG
                     KGKAAAHYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTE
                     AQRAGRVFKDGRYVDAEYPVRQEVYDAFKGVMKAINSSDMPRRSALLDEVRSKPYWST
                     DVEMAARAFERYVQDKARTAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIRDAF
                     DHLFSTLKTRETDKGVAFYSRKGVTRTPEGNLILDVNRSAEAKGSPVPQVEAVARGVM
                     SGIKDSDLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVR
                     EKIRHEIIHHAMEHVVTPAEYQTIIKTVLKTRDSDNAIIREAWRKVDASYGKESPEVQ
                     AGEFLAHMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQR
                     VREGYTPREDGADASSQYSLSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIG
                     RTPPVLRHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATE
                     RNAVVMLLDAVDKNGDPVVSAVHMKATDKRMEINKVASVYGTENGKKLKGMEMAGLTL
                     YRKEKLSRDNPQYSGLQLPKEERSYRGSVDKILYPEDIRKGPYYSRTSSLTPEETIAS
                     RFVRQMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQP
                     LAKLLANY"
BASE COUNT          981 a          883 c         1157 g          792 t
ORIGIN      
        1 gcgattccgt ggtttcacgg gggatgatac ggaggtacag gcccgccttg cccgtggcaa
       61 cgcgccgacg gcggaggagc ttgtacgttc acagatggct gaaggtgatg ccggtccgac
      121 agcacaggag ttaactgagc gtccgcgtct gcccgccccc ggcgatattc atcccggaca
      181 gggatatccg ttaccgggag aggtggcgcg tacgccggat gagaatcagg ccggacgtgg
      241 tggtcgtttt accacaaccg gtgaggttaa gggccagagt ttccagaaag gacgtgctcc
      301 ggcaccggaa aacgccgctg gtcgccaggg ggaaatactg gagggcgaaa cagttcgtcg
      361 tggtctgccg tcaccggatg agcagaacgc cacagcgctg gtacgtgaag gtctaccggc
      421 tccggacatt acacacaatg tccgtatgcc tcagcctgat cagcttcccc gaactgttcg
      481 taactcattg cctgagctcg cacagcaggc agaagtacgt cgacaggccg gaggaaatcg
      541 tgacatcccg cagcctgaga caatcgcacc tgaatctgaa acaacagtcg ctactgacag
      601 ggaagctacc gtgcgcggag gtgaagtcag gggcaaaaaa attgaagact ttggtgaaga
      661 aattaagggc gcggcaaaac accgttatgc acagcttgct gaaacactgg gtaaaacgct
      721 ggaagacagg gattatgtca cgcaaccgct gagcaaactg ttcccgaaac cggactacgc
      781 aaaactggcg aacgaaggtg ccgatgctga cactctggca atgatagcgc tgtatcgtag
      841 cgatattccg gcgaagacga aacacaatat ggcaggctgg ggggagagcg taaaaaaagt
      901 acgacacagt gtatcggaaa tgctgaacgg aacggtcagc gcgaaacgcc tcgcagaatg
      961 gatggaaggc agaatgccct cccgttacgc ggatacctgg caactgttac gcacgctgcc
     1021 accttcacaa atggacaagg cttctgctta tcgggtggta tccggtgtgt atcaagcggc
     1081 cggagggaag cgttacgatc cgccacagaa actttattca ctgcgcaata aggacaataa
     1141 ggggagtaac ctctttttct cggaaagcag ggatgaatta ctgacaaagg cgaaagcctg
     1201 gtttgcagag caggaggaaa agtcacaggc gaaaggtgat gaaaaaacga caccgtcacc
     1261 ggacgacaaa atccgttttg acgtttaccg gagtacccgc agtggcgata tttttatcgc
     1321 ttacggcaaa aacaaaatgc gggttagagg tggttttaag tcagccagtg atgcgcgtaa
     1381 gtacattgat tcacatcgtg atgagcttgt tcgtcatgtg aaggagatga gggaggtttc
     1441 acgtgaggag cagcgtaacg ccaccaaccg tgatcgtacc ggaccagaac gccgcaaagg
     1501 ggatgtttcg ccggagcagt tcagtgatgc gtttggtttc cgtggtgtgc agtttggtaa
     1561 ttatgtggaa ggtccgcgtc gtcaggctga tttgaaccgg gcttatgact cgctgcatga
     1621 cctggctgac gtgctgaacg tgccgacaaa agcgctttcc ctgaacggtc gtcttgggct
     1681 tgcttttggt gcccgtggta agggtaaggc ggcggcacac tatgagccgg gtgaggtggc
     1741 aattaacctg acaaaaggta acggaccggg ggcgctggcc cacgaatggt tccattcact
     1801 ggataattat tttggtcgtt atgatgtttc cactgacggg aagatcacgt caggtggcga
     1861 ctatatgacg gaagcacagc gtgccgggcg cgtatttaaa gacggcaggt atgttgatgc
     1921 ggaatatccg gtacgtcagg aggtttacga cgcgtttaaa ggcgtgatga aagcgatcaa
     1981 cagcagtgac atgccgcgtc gttcagcgct tctcgatgag gtgcgctcaa aaccgtactg
     2041 gtcaacggat gttgaaatgg cggcgcgtgc ctttgagcgt tatgttcagg ataaagcgcg
     2101 tacggctggc gtggagaatg attatctggt caatatccgt aaggcacctg agcacaacac
     2161 agataacacc tacgcttatc cgacgaatgc ggaactggat ggcggtattc gtgatgcatt
     2221 cgatcacctg ttcagtacgc tgaaaacccg tgagacggac aagggagttg cgttttattc
     2281 ccgcaagggc gtgacccgca cacctgaagg caatctcatt ttggatgtta accgcagtgc
     2341 ggaagccaaa ggcagcccgg tcccgcaggt tgaagcggtt gcccgtggcg tgatgagcgg
     2401 cattaaggac agtgacctga aggtccgtgt ggtgaagtca cagaaagagg ctgaagcgct
     2461 ggcgggtgaa tcgttcgacg gttacggcag ggtgcacgcg ttctatcgtc cggataaacg
     2521 tgaaattgtc ctggtggcgg ataacatccc tgacgggcgg accgtacgcg agaagatacg
     2581 tcacgagata attcaccatg ccatggagca tgttgtcacg ccagcggaat atcagacgat
     2641 tatcaaaacc gtgctgaaaa cccgtgacag tgataacgcc atcatccgtg aagcctggcg
     2701 taaggttgat gcgtcctatg gtaaggaatc accggaagtg caggcgggtg aatttctggc
     2761 gcatatggca gagaagcagc cgaataaatt cgtggcggca tgggagcgtg ttgttgccct
     2821 ggtcaaaggg gtactgcgtc gtacggggtt actgaagccg acggaactga acgatatcag
     2881 acttgttcgc gaaactattc gcacgttagg ccagcgtgtg cgggaaggtt acacgccgcg
     2941 tgaggatggc gcggacgcat cgtctcagta ctcccttagt ggtaaacctg atccgttcaa
     3001 agtgccggaa ggtgagggtg agcgttatcg tgatgacctt gccagaatga tgaagtcgtt
     3061 acgttctggt gcaatgactg caaacatcgg gcgtacgccg ccggtattgc gccaccttgg
     3121 cgctccagat ttgccgctgg ttatctcccg cgacactgtg cggaaggcaa ccaatggagt
     3181 gaaacatgtg gtgccaatgg atgttatcga gagactcccg gaactgatgc atgatccgga
     3241 tgcaatttac cgttccgcga cagaaagaaa tgcggttgtg atgctgcttg atgctgtgga
     3301 taaaaatggt gatccggtgg tgtcggctgt gcacatgaag gcaacagata aacgcatgga
     3361 aattaacaag gtggcttctg tatatggtac ggaaaacggg aaaaagctga aaggcatgga
     3421 aatggctgga ttaacgcttt accggaagga aaaattaagc cgcgataacc ctcagtacag
     3481 tgggctccaa ttgcccaaag aggagcgttc ttatcgcggc tctgttgata aaatactcta
     3541 tcctgaagat attcgcaagg ggccgtatta ctcccgtacc agcagtctga cgccggaaga
     3601 gacaattgca tcccgttttg ttcgccagat gcaggataaa ttccaggtgc tgaaggcggt
     3661 gcaggagaat atccgtaaaa ctggcggaaa aatagatgac agcaataacg cctatatggc
     3721 tgaagaactc ttccacggga aggcggaaaa cgacctgaac gtaatgaagg agcgctacgt
     3781 tcagccgctg gccaaattgc tggcgaacta caa
//
LOCUS       DAZKHK010000260         3776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKHK010000260 DAZKHK010000000
VERSION     DAZKHK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.262"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..1209
                     /gene="rfaQ"
                     /locus_tag="ACHOS5_005117"
     CDS             151..1209
                     /gene="rfaQ"
                     /locus_tag="ACHOS5_005117"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP9779770.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKITNFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1206..2330
                     /locus_tag="ACHOS5_005118"
     CDS             1206..2330
                     /locus_tag="ACHOS5_005118"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9779771.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYTAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2323..3120
                     /gene="rfaP"
                     /locus_tag="ACHOS5_005119"
     CDS             2323..3120
                     /gene="rfaP"
                     /locus_tag="ACHOS5_005119"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP9779772.1"
                     /translation="MVELKEPFATLWRGKDPFGEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWAINPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3136..>3776
                     /locus_tag="ACHOS5_005120"
     CDS             3136..>3776
                     /locus_tag="ACHOS5_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9779773.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPEASVASSFHVAYGIDQNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPDADIQRLSQLAKSYHTCIQIHLVNCERLKVLP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGTLKPLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVS"
BASE COUNT          988 a          823 c          924 g         1041 t
ORIGIN      
        1 tcattcgagt cggattgccg cgaaagaagt gatacactag cgcgtcaata tcagtgcggt
       61 atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg cttcacctta
      121 atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag aattttgctc
      181 attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag ttcgctgaaa
      241 aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat cccgatcctg
      301 tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca
      361 gaaaaaatta ccaacttttt tcatctcatc aaggtattac gtgccaataa gtatgacctt
      421 atcgttaatc ttaccgatca atggatggtt gctatactgg ttcgcttatt aaatgcccgt
      481 gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa tagtttcact
      541 catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt gctgacgcca
      601 ttgggacttg aatcgttggt gaagcagaca accatgagtt acccgcctgc aagctggaaa
      661 cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt tatccaacct
      721 acggcgcggc aaatcttcaa atgctgggac aacgccaagt tttccgctgt gattgatgcc
      781 ttacatgctc gtggttatga agtcgttctg acgtccggcc cagataaaga cgatctggcc
      841 tgcgtcaatg aaattgcgca gggatgccag acgccaccag taacggcgct ggccggaaag
      901 gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat tggcgttgat
      961 tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg
     1021 acagaccata ttttctggcg tccctggtca aataacatga ttcaattctg ggcgggagat
     1081 taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct ttcggttatt
     1141 ccggcggcag atgtcattgc agctgtcgat aaattacttc cctcctccac gacaggtacg
     1201 tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt ttgcagcgcg
     1261 attttatgcg tattgctcag acagtcgccg cccgaggtca tcatgttcgg gtttataccc
     1321 agtcgtggga aggcgaatgc cctgatgtat ttgaactgat caaagtgccg gttaaatcgc
     1381 ataccaatca cgggcgcaat gcggaatatt ttgcctgggt gcaaaaacat ttacgtgaac
     1441 accccgtcga tagagtcgtt ggatttaata aaatgccggg gctggacgtt tattatgccg
     1501 ctgatgtttg ttatgccgag aaagtagcgc aggagaaagg ctttttctat cgcctgacgt
     1561 cacgttatcg ccattatacc gcctttgagc gggcaacctt cgaacagggc aagccgacac
     1621 agctgctgat gctgacagat aagcaaatcg ccgatttcca gaaacattat cagactgaag
     1681 cggagcgttt tcatattctg cctccgggga tttatcctga tcgtaaatat agccagcagc
     1741 cagccaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa caatatttat
     1801 tgttgcaggt tggttcagac ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg
     1861 cttcgttacc ggattcgctg cgccacaaca cattactata tgttgttggg caggataaac
     1921 cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt cacttcttct
     1981 cggggcgcaa cgatgtctcg gaattaatgg cagcggcgga tttattactt catcctgcct
     2041 accaggaagc cgcaggaatt gtactgctgg aagcgataac tgcaggatta ccggtactaa
     2101 caacagccgt ttgtggctat gcgcattata ttgtcgatgc caattgcggc gaggctattg
     2161 ctgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta acgcaatctt
     2221 cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa gatttataca
     2281 gtctgccaga gaaagcggcg gacatcataa cgggtggtct ggatggttga acttaaagag
     2341 ccgtttgcca cgttatggcg cggtaaagat ccttttgggg aagttaaaac cttgcagggt
     2401 gaggtatttc gtgaactgga aactcgccgc actctgcgct ttgaaatggc gggcaaaagc
     2461 tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa tttactctca
     2521 ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg actgcgggat
     2581 gtcggcgttg atactatgta tggggtggca ttcggcgaaa aaggtattaa tccgctgacc
     2641 agaacctcgt ttattattac cgaagatctg acaccaacca tcagtctgga agattactgt
     2701 gctgactggg cgatcaaccc accagatgtt cgcgtaaagc gtatgcttat taagcgtgtc
     2761 gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc accgtgactg ttatatctgt
     2821 catttcctgc tgcacttgcc tttttccggt aaggaagagg agttaaaaat ttcagtaatt
     2881 gacctgcacc gggcgcagat acgtgcaaaa gtaccgcgtc gttggcggga taaagatctt
     2941 attggacttt atttttcttc gatgaatatt ggcctgactc agcgggatat ctggcggttt
     3001 atgaaagtgt attttgtcgc cccgcttaaa gacattatca agcaggaaca agggctgctg
     3061 tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa
     3121 tttccggaga gaaatatgag tgcccactat tttaatccac aagagatgat caataagaca
     3181 atcatcttcg atgaaaggcc agaggcgtca gtggcatcat cattccatgt tgcttatggc
     3241 attgatcaga actttctttt tggttgtggt gtttcaatca cgtcagtttt gttacataac
     3301 aacgacgtga gttttgtctt ccacgttttt attgatgata tccctgacgc cgatatccag
     3361 cgtttatccc aattggcgaa aagctatcat acctgtatcc agatccatct ggtaaattgt
     3421 gaacggctta aggtattacc gacgaccaaa aattggtcta ttgccatgta tttccgtttt
     3481 gtaattgcag attactttat tgatcaacaa gataagatcc tgtacctgga tgctgatatc
     3541 gcctgtcagg gaaccttaaa accgctgata acaatggatc ttgccaataa cgttgctgct
     3601 gttgttactg aacgcgatgc taactggtgg tcgttacggg ctcaaagtct gcagtgtaat
     3661 gaacttgaaa agggttactt taattcaggt gtcctgttaa ttaatacact agcgtgggcg
     3721 caggagtccg tttctgctaa agcgatgtcg atgcttgctg ataaagccgt cgtttc
//
LOCUS       DAZKHK010000261         3774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKHK010000261 DAZKHK010000000
VERSION     DAZKHK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.263"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..1389
                     /gene="alaC"
                     /locus_tag="ACHOS5_005121"
     CDS             151..1389
                     /gene="alaC"
                     /locus_tag="ACHOS5_005121"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP9779774.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1454..1525)
                     /gene="ypdK"
                     /locus_tag="ACHOS5_005122"
     CDS             complement(1454..1525)
                     /gene="ypdK"
                     /locus_tag="ACHOS5_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP9779775.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1881..2801)
                     /gene="lpxP"
                     /locus_tag="ACHOS5_005123"
     CDS             complement(1881..2801)
                     /gene="lpxP"
                     /locus_tag="ACHOS5_005123"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP9779776.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3154..3396
                     /gene="yfdY"
                     /locus_tag="ACHOS5_005124"
     CDS             3154..3396
                     /gene="yfdY"
                     /locus_tag="ACHOS5_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP9779777.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3473..3748)
                     /gene="ypdI"
                     /locus_tag="ACHOS5_005125"
     CDS             complement(3473..3748)
                     /gene="ypdI"
                     /locus_tag="ACHOS5_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP9779778.1"
                     /translation="MKVNLMLFSLFLFVSIMAWNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITSTYPTKANYLIHPLR"
BASE COUNT          965 a          882 c          949 g          978 t
ORIGIN      
        1 ttttgaagtg gagttcctca tttaaagcca ttgataaaaa tgttctaaaa cgctggacag
       61 acagaaatta atcaggctat tttcaaaaca gccaccatcg tggcgacgtc gctcggatgg
      121 tccgggcgct aacgttaatc tgaggaaatt atggctgaca ctcgccctga acgtcgcttt
      181 acgcgtattg atcgtctccc gccctacgtt tttaacatta ccgctgaact gaaaatggct
      241 gcgcgtcggc gcggcgaaga tattatcgat ttcagtatgg gtaacccgga cggtgcgact
      301 ccgccgcata tcgtcgaaaa attatgtacg gtggcccagc gcccggacac gcatggttac
      361 tccacttcac gcggcattcc gcggttacgt cgcgccattt cccgctggta tcaggatcgc
      421 tacgacgttg aaatcgaccc ggaatcagaa gccatcgtca ctattggttc gaaagagggc
      481 ctggcgcatc tgatgctggc gacactggat cacggtgaca cagtgctggt gccgaatcca
      541 agttacccca ttcatattta cggcgcggtg attgccgggg cgcaggtacg ctcagtgccg
      601 ctggtggaag gtgtcgattt cttcaacgaa ctggaacgcg ccattcgcga aagttatccg
      661 aaaccgaaga tgatgatcct cggcttcccg tcgaacccaa ccgcgcaatg cgtggaactg
      721 gaattctttg agaaggttgt ggcgctggcg aaacgctacg atgtgctggt ggtccacgac
      781 ctggcctatg ccgatatcgt ctacgatggc tggaaagcgc cgtcaatcat gcaggtgcca
      841 ggtgcacgcg acgtggcggt cgagttcttt acgctgtcga aaagctacaa catggcgggc
      901 tggcgtatcg gctttatggt cggtaacaaa acgctggtca gcgcgttggc acgtattaaa
      961 agctatcacg attacggcac ctttacgccg ttgcaggtgg cggctattgc ggcgctggag
     1021 ggcgatcaac agtgcgtgcg cgatattgcc gaacagtaca aacgccgccg tgatgtgctg
     1081 gtgaaagggc tgcatgaagc gggctggatg gtcgaaatgc cgaaggcttc gatgtatgtc
     1141 tgggcgaaaa tcccggaacc gtatgctgcc atgggctcgc tggaatttgc caagaaattg
     1201 cttaacgaag cgaaggtctg tgtctctcca gggattggct ttggcgacta cggcgatacg
     1261 catgtgcgct ttgcactgat tgaaaaccgc gaccgtattc gtcaggcgat tcgtgggatt
     1321 aaagcgatgt tccgtgccga cggtttacta cctgccagca gcaaacatat tcacgaaaac
     1381 gcggaataaa caccatcgtc agaaatgcaa acaggagcca gatggctcct gtttttcatt
     1441 gcacagtttg cttttagatc atcagggcaa aagtaccggc ccaaacgatg accataaaag
     1501 aaatgcccat aaagaaatat ttcactttac atctctccgc gtaattactg ttacgcctga
     1561 atgactttat tacacctgat acagtgagtt gacaccgggc aaaacgacct taaacgtcct
     1621 gtattttacc gtgggcatca tcccagaatt ctgagctaaa ctgctcgaga cggcgctaat
     1681 gtacgcctaa aactgaaacg agacaagagg cattttctta tttactttta gtaacttaca
     1741 ggtgtctcat atcggcgaca gtttaatttc tgacggtaat aattagatat tcagcgaagg
     1801 aaaaaacctt aagaaaaaag ccataaaaac catgaggtta ttatggctga tttgaggagg
     1861 gaaagagtga gagccgtttg ttaaatgtat aacgacgatt cacccaccgg gcgcgtttta
     1921 aagcgacggt ggatccagag gtactgctcc ggtgcgcgca tgatctcttt ctcgataatc
     1981 ttgttcatat aggcagcggc ttgattttca tctgtcgggt agccttccat ctctggggtg
     2041 atgtacaaac gatatccgct gtaatccgct tttcttacca tcgttacggt caacatggct
     2101 gcgccagaga gacgagagag gacataggtg ccattggttg tggcgacatt ttccaccgca
     2161 aagaacggcg cgaaggagct gcctttacga ccataatcct gatcgggagc aaaccatacc
     2221 gcttcacctt tcttcagtgc actgacaatg ccgcgcagat tatttctgcc gatcatcgct
     2281 ttgttagagc gcatgcgacc acgggtctgc acccattcca tcagctgatt attatgcggg
     2341 cgataggtgg ccatcattgg ttggcaaagt cccattaccc gaccgcccag ttccagcgac
     2401 ataaaatgga cgccgacaac cattacgccg cgattttgca tttgtgcgcg tttaaggtta
     2461 tccagccctt ccacatcaaa ccatttacgt acgcgactgt cgggccagaa ccacgccatg
     2521 ccggtttcta ccagcgccat gccgagtgaa cgaaagtttt cggcaatcat cttctcgcgc
     2581 tctttcgcag aatgctgcgg gaaacaaagt tccaggtttt tacgggcgat agattcacga
     2641 cgtttcagga atggtcgcgc cattgtgccg atacgcgtgc cgagaaagca gagaacagga
     2701 taaggaagct gtacccaaag ccagagtaca ccaagcccaa accacgtgag ccagtagcga
     2761 ggatgcagaa actcgcggga aaatttgcat tttggaaaca tagttacctt ctgtagatac
     2821 gaattgttgt atcgcagaag attccatgtc gacagcggta cactcaattg attaggccat
     2881 catgtacgac aatggttcct gtaaagagtg taaagactag tcaggacata accacgagga
     2941 gcatgaatgc ggtatctgta ctttatcact cgctgactgc tttcagctac tgattattaa
     3001 tcgtgcaaag taaatatttt tccgtacaca acagtcttgc atttaacatg ctgttttcat
     3061 tgatgcttag caaaaattgc cagaggttaa ggattgtctt actattaaag tgactgaagt
     3121 tacaggtttt ttagcgcggc aggaggttga gctatgattt atttatggat gtttctcgcc
     3181 ctgtgtattg tctgcgtaag cggatacatc ggtcaggtac tgaacatggt atcggcgatc
     3241 tcttctttct tcggcatggt gatcctcgct gcactcattt attacttcac catgtggtta
     3301 actggcggta atgaactggt gacggggata tttatgtttc ttgccccggc ttgtggcttg
     3361 atgattcgct ttatggtggg gtatggcagg cgatagtggg tcaggatggc tcatttgggc
     3421 aggtgttcac atcagcatca gacatgattg ttcacctgcc aggtgacaga cgctatcgca
     3481 aaggatgaat gagataattc gcctttgttg gataagtaga cgtgatgtct tctgtgaggt
     3541 ttaatggtgg tgcatcttct ctcatcttaa acaaaatcgc ctgtgatggc tgaagttttg
     3601 tagaatgcgt tgtcgtcatc actgacgttt tttctgtctc tttatagctc gcctcactac
     3661 caccaccaga aatagaaaat gcaaaaacat tccatgccat aatagagaca aataaaaata
     3721 agctaaaaag cattaagttt actttcatag aaaatatcct gaaaatggtt cact
//
LOCUS       DAZKHK010000262         3753 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKHK010000262 DAZKHK010000000
VERSION     DAZKHK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.264"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            12..515
                     /locus_tag="ACHOS5_005126"
     CDS             12..515
                     /locus_tag="ACHOS5_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779779.1"
                     /translation="MNRRKYVLFSLFSIAFMATTANAAVCTPAMETAVKQAQYNAMKI
                     DAAQLEPIAEGFMANINSSLDTIGCTDVWPTGSIGINLPSFDTIIRKAKEAAISKACS
                     IAREKMNSVMGNISESVSFDVPGMGNIAGGSISTGTGSTAGGSINVNGNSTTGSDVWN
                     SISDVMK"
     gene            603..3236
                     /locus_tag="ACHOS5_005127"
     CDS             603..3236
                     /locus_tag="ACHOS5_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFP9779780.1"
                     /translation="MRTGYIAAAILFMCLLITQNAYAADLFTVPDTDKSKLWFLDVLF
                     PENLAQSPLASTMTILNSAILLVGGILAAYTLIAGTMSTAHDGEMLGKKWSSMWLPVR
                     TALGTAMILPAAGGFCAAQVMVLWLINQGVGLADTVWNTYAANPSDGAVITTSASYQE
                     LDRIAKTAFINNVCMLKAGQLWKKSHESALFPGVMPVFEMTSEKGKYLYRYNYGANNV
                     GNEMNKNLLVSKSACGSITLTDPEAKASYDEQVQAHAQATIASGGMYMSYMPQEIKTN
                     ISAVVEAHNTAFIALNNNMMTLAEKYVADNNIDIQSDINSATATYVSVIDTAVRTTFS
                     TGNQWDDFKDNVQKDGWFMAGAWSMKLIRIQDAINGAAHNLPVAGQQSMDYGDIFNSL
                     NAVMAKVSHDMARSTTASRYANGIDAQNRTEANTTGKGGQGSKTDDASKIITSLKSEA
                     DKSISGAMAGFLSSSVINGRKQGKLVAFSTDTTAANLQAINPLLAVKGLGDTLSTAGW
                     SLLGGSAVVGAGLGIWTSFTANWLSGAFGAIGVVMPLVIPLWVAGATLAVVIPMLPYV
                     MWFGVCVGWMILCLEAMVAAPLWVITHLHPDGDGVVGRGGAGYGLVLSLTMRPALMVT
                     GLIAAYTMLPILGGVINETFSGAFGMMSANAGISIIESLALIAVYIALMFTVVKKSLS
                     LIHVIPDEIMKWLGVHSGQSMSGYAQSASKGVESAVLAKTVLDQLSHSSNALGNQIRN
                     GQMNKDREQQRELQQQQQVQQGKAQASSRASDTGSAFRTHMSNAGPLDQQDEYQSLES
                     ANSAYSAAEAADAVGDTAGAAGYMDVAQKAANRAVSFGEHNRPLLPLNLQSKASPIES
                     FKTPGKGDGGGSSGGSEEPSQ"
BASE COUNT         1114 a          712 c          908 g         1019 t
ORIGIN      
        1 aggagtaaaa aatgaataga agaaaatatg ttttattttc actgttttct atcgcattta
       61 tggcgacaac agcaaacgct gctgtttgca cacccgctat ggaaaccgca gtaaaacagg
      121 ctcaatacaa cgcgatgaaa atcgatgcag ctcaactgga acccatagcc gaagggttta
      181 tggcaaacat aaatagttcc ttagatacta ttggttgcac cgatgtatgg cctactggaa
      241 gcatcggcat aaatctgcca agtttcgata ctataatcag aaaagcaaaa gaagcagcaa
      301 taagtaaagc ctgttcgatt gccagagaaa aaatgaattc tgtgatgggg aatatatcgg
      361 aaagtgtttc ttttgatgtt ccgggtatgg ggaatattgc agggggtagc atttcaacag
      421 gaacaggatc aactgcggga ggaagcatta atgttaatgg aaactccaca acaggttctg
      481 atgtatggaa cagtatcagt gatgtgatga agtaaaatat aaaaaatgta actcacttgg
      541 tgcgttgcat aaaaaaggga gaaaaaatga aaataaaaat ggagcaatgc tctttgaata
      601 tcatgaggac aggatacatc gcggcagcta ttctttttat gtgtttgctg attacgcaaa
      661 atgcatacgc agcagattta tttacggttc ctgatacgga taaatcaaaa ctgtggttct
      721 tggatgtttt atttcctgag aatttagccc aaagcccgtt agccagcaca atgacaatcc
      781 tcaacagtgc cattttgttg gttggtggga ttttagcagc atacacgcta atagccggaa
      841 cgatgtcaac ggcgcatgat ggcgaaatgc tgggtaagaa atggtcctct atgtggcttc
      901 ctgtcaggac agcgttagga accgcaatga tacttcccgc agccggtggg ttctgtgcag
      961 ctcaggttat ggtgttatgg ctgataaatc agggcgtagg tcttgccgat acggtctgga
     1021 atacgtatgc cgcaaatcca tcagatggtg ctgttatcac cacgtcagcg tcctatcagg
     1081 aacttgaccg catagcaaag acggcattca taaataatgt ttgtatgttg aaggcaggcc
     1141 agctatggaa aaaatcgcat gagagtgcgt tgtttcccgg tgttatgcct gtgtttgaaa
     1201 tgacatcgga aaaaggaaaa tatttatacc gatacaatta cggtgctaat aatgttggta
     1261 atgaaatgaa taaaaactta ctggtatcca aatcagcgtg tggaagcatc acattgacag
     1321 acccagaggc taaagcgtct tacgatgaac aggttcaagc tcacgctcag gcgactatcg
     1381 catcaggtgg aatgtatatg agctacatgc cgcaggaaat caaaaccaat atatcagccg
     1441 ttgttgaagc tcataataca gcttttattg ccctgaataa caatatgatg acactggcag
     1501 aaaaatatgt cgctgataat aacatcgata ttcagtctga tataaacagt gcaactgcta
     1561 cctacgtttc tgttatcgat acggcggtca gaactacatt ttctacaggc aaccagtggg
     1621 atgacttcaa ggataacgtc cagaaggatg gctggttcat ggcgggggca tggagtatga
     1681 agttgatccg cattcaggat gcaataaatg gtgctgctca caatcttcct gtcgcgggac
     1741 aacagagtat ggattatggc gatattttta acagtctgaa tgctgttatg gcaaaagtgt
     1801 ctcacgacat ggcgagatcg acgacggcat caagatacgc gaatggtatt gatgcgcaaa
     1861 accgaacaga agccaatacc actggtaagg ggggacaggg gtctaaaaca gatgatgcca
     1921 gtaaaataat cacttcactc aagagtgaag cagataagag tatttctggc gcaatggctg
     1981 gtttcctgag ttcaagcgta atcaatggca gaaaacaagg aaaactcgtc gcattttcaa
     2041 cagatacaac ggcagcaaat ttacaggcta tcaaccccct gttagccgtt aaaggccttg
     2101 gcgatacact cagtaccgcc gggtggagtt tattaggtgg ctctgctgtt gttggtgcag
     2161 gtcttggaat atggacgtca tttactgcaa actggttgtc tggagccttt ggtgcaattg
     2221 gtgtcgttat gccactggtg atcccgctat gggttgctgg tgctacatta gctgttgtta
     2281 ttccaatgct gccatacgtc atgtggtttg gtgtctgcgt gggctggatg attctgtgcc
     2341 ttgaagcaat ggtggcagca ccattatggg taatcacaca cttacatcca gacggagatg
     2401 gtgttgtagg acgaggcggt gctggctatg gtctggtgct gtcgttaact atgcgcccgg
     2461 cactgatggt aacgggacta atagccgctt atacgatgct tccgatactt ggtggggtga
     2521 ttaatgaaac gttctccggc gcgttcggta tgatgtcagc caacgccggg attagcatca
     2581 tagaatcact ggccctgatt gctgtttaca ttgcgctgat gtttacggtt gtgaagaaat
     2641 cgctttcgct gatccatgtt attccagatg aaattatgaa atggcttggc gttcatagtg
     2701 gtcagagcat gtcgggttat gctcagtcgg catcaaaagg tgtggaatcg gcagtactgg
     2761 caaaaacagt attagatcag ttatcacatt cttcgaacgc gttaggtaat cagatcagaa
     2821 acgggcaaat gaataaagac cgtgaacaac aacgtgagct tcaacagcag caacaagttc
     2881 aacaagggaa agctcaggct tccagtagag ccagtgatac tggatctgca ttccgcaccc
     2941 atatgagtaa tgcaggaccg cttgatcagc aggatgaata tcaatccctt gaaagtgcca
     3001 actctgctta cagtgcggct gaagctgcag atgctgttgg tgatacagcg ggagctgctg
     3061 gctatatgga tgtcgctcaa aaagcagcta acagagcggt atcttttggc gagcataaca
     3121 ggccattgtt gccactaaat cttcaaagta aagcgtctcc tatagagagt tttaagactc
     3181 cgggtaaagg tgatgggggc ggatcttctg gtggaagtga agaaccgagt caatgatgaa
     3241 taaaggtatt agagggcttc ggtcctcttt ttaatggatt gtatccttca tccttattgt
     3301 attttcgcct ttgacgtgtt tttaggataa atacatcatc aacaggggca atctcttaga
     3361 gttgctacac aaaatatagt tttcctatct atttaagaac aaaatctata tatctaagta
     3421 ataaactaac taactaacta actaactaac taactaacta actaactaac taacttagtc
     3481 tcagtaggtt taatatgaat gtgttgctgt tctttaacag accctaccgg gagtgttgtt
     3541 aaacctgaga ggggcaacat ttcctaattg tcattagttc tttcttcata tttctggaac
     3601 gcagattgta ttcaaggtat ctttagcgaa gtgaaagttt ctacatttca tgaatgtcaa
     3661 actgaatgtt agaaaaggaa tatctcatat acatacgatt tataactcgg gagatgacat
     3721 gttaagaatc aatcaaatag taaagatcct tgg
//
LOCUS       DAZKHK010000263         3688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKHK010000263 DAZKHK010000000
VERSION     DAZKHK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.265"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..269)
                     /locus_tag="ACHOS5_005128"
     CDS             complement(<1..269)
                     /locus_tag="ACHOS5_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9779781.1"
                     /translation="MGRPDWRAMLAGMSSTEYADWHRFYSTHYFHDVLLDMHFSGLTY
                     TVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAGGVR"
     gene            complement(296..718)
                     /locus_tag="ACHOS5_005129"
     CDS             complement(296..718)
                     /locus_tag="ACHOS5_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9779782.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            complement(734..1474)
                     /locus_tag="ACHOS5_005130"
     CDS             complement(734..1474)
                     /locus_tag="ACHOS5_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779783.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVRRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            complement(1482..1877)
                     /locus_tag="ACHOS5_005131"
     CDS             complement(1482..1877)
                     /locus_tag="ACHOS5_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9779784.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDVPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(1874..2452)
                     /locus_tag="ACHOS5_005132"
     CDS             complement(1874..2452)
                     /locus_tag="ACHOS5_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779785.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(2464..2817)
                     /locus_tag="ACHOS5_005133"
     CDS             complement(2464..2817)
                     /locus_tag="ACHOS5_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9779786.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(2829..3224)
                     /locus_tag="ACHOS5_005134"
     CDS             complement(2829..3224)
                     /locus_tag="ACHOS5_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9779787.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEEFDDT
                     DDAAGQDTSVSPENVLTVHENEVVSAQPDTVTDTAELVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGMAAEMTERGLARMQ"
     gene            complement(3266..>3688)
                     /locus_tag="ACHOS5_005135"
     CDS             complement(3266..>3688)
                     /locus_tag="ACHOS5_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9779788.1"
                     /translation="PKGWALFRSFKAVREKLDTRRGSHSELETAVKDLGKAVSYKGMY
                     GDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTHARGLRTYGCIQDADALSEGINASPR
                     YPKNWKTSGDPAREFTMIQSAPLMLLADPDEFVSVQLA"
BASE COUNT          787 a         1152 c          910 g          839 t
ORIGIN      
        1 cggacgcctc cggcaagccc tgccgctttc tgcatcagca catcatcttc aggctcttcg
       61 tcagcctcgc gccggttcag cagactgaaa tccagcggat gcatatccgg atcgctgaaa
      121 aacaggctga gtacggtgta cgtcagcccg gaaaagtgca tatccagcag aacatcatga
      181 aaataatggg tactgtaaaa gcggtgccag tcggcatact ccgtggatga catcccggca
      241 agcatggcgc gccagtcggg tcgccccatc tcacgcgcca gtttcagggc aaaactcagc
      301 tcaccgtcga acactttccc gcagaaacag gctctgctgg cccggcgtca tctgcctgtt
      361 caggtgcatc attcacaaca aactcagaca taccggacag acgcattacc acattttcag
      421 cctgagcaat tgcctctgtg ggccaggtgg taagcacttc ctgctcaatt tgtttaacgg
      481 cttcattcat ggacggcatc tttgtcttct gcggatggct atgccacagg gacatcgcca
      541 ccagaaaagc accggttctg acgagatctt ccacgctcac ctgtcgattg agactggatc
      601 ccgcctgttc tgcctgtcgt ttcagcaggg cgagatgctc aattcgctgc agggctgaca
      661 gttcagaaag cgtgacgctc acaccgttat attcaaatga ttcggttttc aggaacatcg
      721 ctgactctcc ggattaactg tcggtgacgg tgatttctgc aaccacagca agttcaccat
      781 taccggatac aaccggaatg ttgaccttgc ctgcagcaac acctttcacg gtgatggtca
      841 taccactgac cgacacggtg gcttttgttt tatccgcaga caccgcacgg aagctcttgt
      901 cggttgcgcc ttccggctgg aatgccacgg tcagcgtggt gctctgccct ttcaccaccg
      961 aagtgctggc aggcgtcacg gtcatgccgg ttgccgctgt taccgtgctg cgatcttctg
     1021 ccatcgacgg acgtctcacg ttggtgactt tcaccgtgcg ggtgatcact tccttcgccg
     1081 tcaccgcctt accgatactg ctgacccagc cgcggaacac atcgaccgtg ccgttcggga
     1141 agcggatttt ataggcacgg gtatcccctt cattaaacca cgccagcagc gcctgctgcc
     1201 cctgctctcc gggcatccac gccagcgtga agctggtatc tccggcagat ttctgcccct
     1261 gcccggtcgc agtccagtcc gcatcttcat catcgagata gctgtcgtca taggactcag
     1321 cggtcagttc gccgggcgtc aggtctttaa ctttagccag acgcgaccag tcaacgtctg
     1381 aaagcgggtt cgcataaggg tcaccgttcc ccttataaac ccacagtgtg gtcccggccc
     1441 ctttcaccgg cattactgga tttggtacag gcatatcgtc ctcacatttc ataggtaatg
     1501 acataagtca gatcggctga actccacagg cccgcatcat cgtcgcgccg gtagtcatag
     1561 ccactggcca ccatactggt gatcaaatct gacagtgccg ggacatcgct catcaccgga
     1621 taaatccggg actccatcca cgaatccagc tctgaatccg gcacctgagc aggcaggaaa
     1681 acttcaatat gcagctccgc ctgccaggta tcactgtcca gctcttcgcc cgtgtattca
     1741 gcgccggtga gataaacggc aattgccgga aaatccgcct catcaaaaac agcggggcga
     1801 ccatcaaaaa gcgtcgcccc ggtgtcatgc ttctccagtg catccagtac ggctgcacgg
     1861 agttcagtat gtttcatcgc tttattacca ttctcagttg atgctgcagc gcatagccca
     1921 gctctttcgg aagacgttca cgccgtatcc gttcaatatt ctgtttaaac gccgtggtaa
     1981 gcggcaccgc catcgggatt ttcaccacat caatggggta acggtttttc ccggccacac
     2041 gctgcatgac atgccaccgg ccatttttca gttgctgaat aaacgcgccg ggaatacgac
     2101 ggtttcccac cacaagcacg ctgccgccac ctttcaggga tgaacgctgc ccctttttac
     2161 gacgcctgcg gcgggacagg acaacccgcg cattacccag cttgattacg ggcaaatccc
     2221 cccggttaac tttgattctg gcctgcggat ttttgaccgt ggcccttttc agcctggccc
     2281 tttcctttac cagtttccgg cgtacctttg tctcacgggc aacctgtgac gcagactgcg
     2341 atatcgcgga tgaagcaacg cggttaatgg ccattgcggc ggcaccaggc accgccgttt
     2401 tgctgatacg gctgaggttt tcaacggcct gctcaagacc ttttatggcc atacatcccc
     2461 ctttcagcgg cgacggttaa cggcaggcgg tacgccccgt ccaagccaga gatgacaact
     2521 tccgccatca tccggcgaaa cccgatctac ccagaaattt tcctcaccga tggtcagcgt
     2581 gtctccacgc cgcagctgcc gcacctcatc agtccggaca aacagggacg ggctggagcc
     2641 ttcaacgcgc acgccctgtc cggcatagct gatattttca gggtcatcaa aaacaccacg
     2701 tatcaccgca cctgactgct caccggatgt catggtggct gacgttccca tgtacccgcg
     2761 tatcgtttca tcggcgcggg caatggcagc atcgaacagg ttatcgaaat cagccacagt
     2821 gcctcccgtt attgcattct ggccaggcca cgttctgtca tttctgctgc cataccggca
     2881 gagacacgaa acgccgttcc cggcagcaca aatgccacag gttcatcccg cgtggcgtga
     2941 agtgcatcag tatgcagcgt caccagtgcc acgaccgtga ccagttcagc cgtatcagtc
     3001 acggtatccg gctgcgctga taccacctca ttttcatgca cggtcagcac attttccggg
     3061 ctgacagacg tgtcctgacc ggctgcgtca tccgtgtcat caaactcctc ttccagctct
     3121 gccacacgga gcgccagttc ttctttcgtc cccgtcaggc tgacatcacg gttcagttgc
     3181 tcacccagcg aacggagacg ggcaatcagt tcatctttcg tcatggactc ctccacagag
     3241 agaaaatggc cccgaagggc catgattacg ccagttgaac ggacacgaac tcatcaggat
     3301 cagccagcag catcagcggt gctgactgaa tcatggtgaa ctctcgcgcc ggatcgccgg
     3361 atgtcttcca gtttttcgga taacggggag acgcattaat accctcactc aatgcatctg
     3421 catcctgaat acagccatag gtgcgcagac cgcgtgcatg agtgttaccc agcaccatcg
     3481 tgttgtccgg caggaagttc tttttgacgc cgttttccac gtactgtccg gaatacacga
     3541 cgatggccac atcgccatac attcccttat aagacaccgc tttgcccagg tcttttaccg
     3601 ctgtctccag ttcggaatga gagccgcgac gggtatccag cttctccctg acggctttga
     3661 aggaacggaa cagcgcccag cctttcgg
//
LOCUS       DAZKHK010000264         3601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKHK010000264 DAZKHK010000000
VERSION     DAZKHK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.266"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..218
                     /locus_tag="ACHOS5_005136"
     CDS             <1..218
                     /locus_tag="ACHOS5_005136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779789.1"
                     /translation="KNNTSKPPDQQVSHRLSEQQNRFAGGWSHCVTPTTITSVFSRTV
                     VNHKIGTHAGGAGPHDFLFDESENGER"
     gene            509..703
                     /locus_tag="ACHOS5_005137"
     CDS             509..703
                     /locus_tag="ACHOS5_005137"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP9779790.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIDWCIARAEYCKKTGRF"
     gene            complement(2259..3275)
                     /locus_tag="ACHOS5_005138"
     CDS             complement(2259..3275)
                     /locus_tag="ACHOS5_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP9779791.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYESDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLIGADIPAELDEFEEQAFWLAFDWGRTEKRYKRNPEAD
                     RMKD"
BASE COUNT         1016 a          907 c          771 g          907 t
ORIGIN      
        1 aaaaaaacaa cacatccaaa ccacctgatc agcaggtttc tcaccgactt tctgaacaac
       61 aaaaccgctt tgcaggtggt tggtcccact gcgtgacacc aaccaccatc accagtgttt
      121 tttctcgtac cgtcgtcaat cacaaaatcg gaacccacgc cggaggcgcg ggaccccacg
      181 attttctgtt tgacgagtca gaaaacggcg aacgctgaca gatttcaatc tggctgcgtg
      241 agacgttggc ggggtgtcgg ggcgcagccc tgaccaggcg gcatttgtct gattgcgtgt
      301 gcgcggtccg acaaatacac atcctgtccc gtttttcgca cggtttttat tgattaccgg
      361 acagatgaac ccggattgcc atgttgcgaa aatcacgtta aaacgattac gagcgtgcag
      421 ttgggctgat atatcaacag tcatgccaat atgtattaac ccgacaggcc gctctttttt
      481 aattcaaata aaaatataaa ttacgagtat gaatcaggac aaagcaattt atttagaaaa
      541 taaatatttt tcacccgcaa acgaagctgt cagactggag cgtgaaggcg attacattgg
      601 agcatggaaa aaatggaatg atgcgcttca ttatgccaaa ggagaaaata tagactggtg
      661 tatagccaga gctgaatatt gtaaaaagac aggtcggttt taattctgac ggggtttcaa
      721 aggggcgtcc cctttggcac gtcgacgtat caaaatgcgc cagcattttg tatacagtcg
      781 gtcacgtgcc tccaggattt accatcaaaa acgcccctgt agttaatcct gaatcaagat
      841 ataactaaga tataattagc gtataactaa ggcataacta aagtataact aacttagtgt
      901 gcatataatt aagatataac taatatataa ctaatgtata actaacggtt caccaacgga
      961 tcgcatgtag tagacgctac ataaaaaatc ctgaaatgga ggattcgtca taaagagatt
     1021 cctgttttta gcgttctgat gcaggatagg gcaaacaccg caaaatgacg tctttggtat
     1081 gttttgtgat gtttgcttct ggcatctgtt atctgtttta ttgggtgttt ttatgagtgt
     1141 tgatgtaatt aaacaaattc agagtataca ggacaaagga attattattt attcaaaata
     1201 cagagctaac gaatttaaca gggatgatgt atacagagag agttacggcc tgattattga
     1261 gtttaatcat cttattgctg aacatgccgt tcatgatgaa aaactgatta accatactgt
     1321 ctgtattctg aatgaattac gaagcatagc gataaattaa aaaatccccc tggattacag
     1381 ggggaagcca tcagagtttg agtaatgaga aatattattt tggagagagc accacaaatg
     1441 cgctcttttc caatctgccg taaccatcgg taaaagacaa tcaggacagg cgaaaaaatc
     1501 cccctatatg gcaagggggt gtcaatgagg acatggcaat tgtgagtttt gtgtgtttta
     1561 tgtgttgcag tttgcaacgt cagaaatttg ccgtagttat acgtgaaaaa caatcctgat
     1621 ttctggacag gatgtgtaat cgtataaccg cgcacgcacg ccgatacgat taccacctgg
     1681 tcaggtcttt gcccgacacc ctcgacaccg ttcccgcgat acgatagaac atcagggcgc
     1741 gtttccgggt aaacgcatgt taatgaccgg gaattcattg attagggacc ggagacgcgc
     1801 tacaaaactc ctgcgcgttt ctggcatcaa ctgaccatgg ggatggtttg gcagtagcag
     1861 caagcgccag aaacgcgctc aaaatcatca gatgtacagc gaacaaaaaa ttgtcatcgg
     1921 caaaaaatca cgttaaaaca atcgcgagca ccttcctgtc tccttcaatt acggtcaaac
     1981 attttgcatc tgtcgggaag ttgctcacct ccataattca atttctcatt gacaataatt
     2041 ccggtgatat cccctctttt gaggggcttt ttttacccac acaccacaaa cagcagccat
     2101 accatcgtca aaaacgcgct caaaacgtct ctgacgtcct gaaccaacca aaacatacaa
     2161 aaccattgcc caatgcaaaa aagtgcgtta gaaagcgtct gagcgcgttt ctgacgtctt
     2221 ttttgttgct gtgtcgctac tcgttgtcag attcgcgctc aatccttcat cctgtcagct
     2281 tcaggattcc gcttgtaacg tttttccgta cgtccccagt caaacgccag ccagaaggct
     2341 tgctcctcga actcgtccag ttctgccgga atatctgccc cgatcaaatc ctcgtccgtc
     2401 tccggtttat cgactttcag aacctctttg agacgaccgc cggttgccac catgcgaagc
     2461 ccataggtct gccgcgccag ctccagaaac cacgccggat cacgcacaac ttccgaagat
     2521 ttcaccgtgt atttcatcgt ctcaatgacg cctgactcca gcgccgcacg atgcatatca
     2581 ggcagggtta caccatccgg ccgtttccag ccctgtttcg gttttaccac ccgaatatcc
     2641 acacccgcct cataatcgac acgaaggcat tcactccaga tctccaccca gcgagcatgt
     2701 ttcacataac cattaccgga caacatcgaa ggggggacca tcatcagaac gtgaaaatgc
     2761 ggatggcaac atcctggcac cttcccgtaa gtaatttccg tcgtacgtaa ccagccaaga
     2821 actgacctga attccttacg atcactcaag cgtttccatg ctgcattcat ccgaatgagt
     2881 gtttcgcggg tattttccac agggggattt ttgacagtca gcgtcatgaa caaccagcgg
     2941 gccgcaggac gttcaaccag caactttggc aaggcctccc agaggcgacg catccagcgg
     3001 agagagcgac gacgactaca caccggacaa aacggaacat gacaaaactc ccccttccga
     3061 agggccagct tcatttcgcc cgtcaggcgg gacaccttcg gcgcaaacaa caaccgacgg
     3121 gaacaatcaa acatccgttc tccacgagag gaaaacatcg aatcgctttc gtaaatctgc
     3181 gccaccctgt cagccatcac tcgatcactg tcccattttg tatcccgcgt gctgtaatcg
     3241 cgtaacgaga gtaccggact gtctggattt ttcatttgca ccccccaaga actgggcaga
     3301 tgcggaaatt ttgtgttagc atttttctgt cacctgtgac tctgttgccg aacaacgaat
     3361 tccaaactgg tgatcgcccc ctggagtgtg accgcactcg caggggaact ccctcaaaac
     3421 tgttcatttt ttaaccagaa gtttttatct cttttccacc cttttttacc actcaaagcc
     3481 gcgccaccac tggactaagc caaaataaca cagaccgcag ttgttgccta tagtataaat
     3541 acaaggatcg cgcgcgcgat cgcccaccca aaatgcgcgt tttttgagcg ttcccgagaa
     3601 c
//
LOCUS       DAZKHK010000265         3570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKHK010000265 DAZKHK010000000
VERSION     DAZKHK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.267"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..101)
                     /locus_tag="ACHOS5_005139"
     CDS             complement(<1..101)
                     /locus_tag="ACHOS5_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779792.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERY"
     gene            complement(107..805)
                     /locus_tag="ACHOS5_005140"
     CDS             complement(107..805)
                     /locus_tag="ACHOS5_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9779793.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSGADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(805..1134)
                     /locus_tag="ACHOS5_005141"
     CDS             complement(805..1134)
                     /locus_tag="ACHOS5_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779794.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(1131..>3570)
                     /locus_tag="ACHOS5_005142"
     CDS             complement(1131..>3570)
                     /locus_tag="ACHOS5_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9779795.1"
                     /translation="LSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDF
                     NKTLVLSGNQAGLTADRILALSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQ
                     SVARFSSASGVEVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDE
                     AGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQG
                     MLEKAEKAFDEADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQAD
                     LEKAREMAAKDWAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILR
                     ADYNTLMAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGA
                     NEKISQQRRDLWKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKV
                     TYQERLNALAQQADKFAQQQRAKRAAIDAKSRGLTDRQAAREATEQRLKEQYGDNSLA
                     LNNVMSEQKKTWAAEDQLRGSWMAGLRSGWSEWEESATDSMSQVKSAATQTFDGIAQN
                     MAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTA
                     IQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATG
                     GYVGGTGSPAQMRRSEGIRFEQNNNVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQ
                     AQMRDGGLFSGGGR"
BASE COUNT          665 a         1198 c          886 g          821 t
ORIGIN      
        1 aaatatcttt ccccctccgg cgtgcttacc acgaagccgc acgactccgc tggcgcacat
       61 cgccgggcgt gcgccagaat cgctgattct gtctgtgtca tgggatttac tgcgaaagtt
      121 tgttaatgga aaggaagccg ccaaagttgc cgacgttatt gcggaactta caaccgctca
      181 ggcatttgct gcatttatcc ttcgtgatat cggacgttgg ctggtcatat tcatccgcga
      241 cagccggacc gctataaccg cactcgtcac cgcgataggt ccaggtgcag gtgttggcca
      301 gcatgatacg tcccggaaaa acagcgccat ccgtttccgt cggcgtggac agtacaaaag
      361 aggcactcac cgcgctcagt tcgctgcact gctcaatgcg ccagcggctg atcacctcct
      421 gctccggatc ggcgccactg tttccgttga cgaagttcac cgcatccaga aaacgggcgt
      481 aaaccttacg ccggaccacc gttccaccga ccagactctg cagatcttcc gccatcccgg
      541 tgaccatacc gtacaggtta gaaaccgtca gtgtggggcg cgtactggtg cctttgccat
      601 tcagttcaaa accactcccc tgaatgggat acggctgata ctgtcgcccc tgccaggtga
      661 ccggctcacc tttttcgttc tgctcattac agaaaaaata acgttctcca ccgacctctg
      721 tcaggtcgat ttcccagagc accacgctgg ccgactgctc cgcacgggtg cattcattca
      781 gtgtttcctg ccggatatcc tgcatcagtt caccacctgt tcaaactctg cgctgaactc
      841 aacacgcagc atactgaccc gcgacgacca ttttgcgcag gtcaccttta tctgccgcca
      901 ctcataaggc ggcgtccaca gaaaggcttt ccagcccccg tgctcagcca gaaacgactc
      961 cagcgccgtg gcctcccaac ggggaacaga aagcgtcacg ctgtacgttt tcaggtcggc
     1021 attcagcccg gcaggcgctc gctgggaata gccatcacca aagcgcactt tcctgacgga
     1081 aggggccgaa gccacatcca taccgggttt cactttccag cggaaggtct tcatcgtcca
     1141 cctccggaga acagaccacc atcgcgcatc tgtgcctgaa tttcatcacg ggcacccttg
     1201 cgggccatgt catacactgc cttcatcatc tgtggacctg gcagaccatt cgtaccgtcg
     1261 ttctgaatca ccacgttgtt gttctgctca aacctgatac cctctgaacg ccgcatttgc
     1321 gccggacttc cggtgccacc gacataaccg ccggtggcat agccgcgcat cagccggtag
     1381 agatttccca cgccaatccg gctggttgcc tccttcgtga agacaaattc accacggtga
     1441 acaatccccg ctggctcata tttgccgccg gttcccgtaa atcctccggt tgcaaaatgg
     1501 aatttcgccg cagcggcctg aatggctgta ccgcctgatg cggatgcgcc gccaccaaca
     1561 gccccgccaa tggcgctacc gatactcccg acaatcccca ccattgcctg cttaagcaga
     1621 atttctgtca tcatggacag cacggaacgg gtgaagctgc gccagttctg ctcactgccg
     1681 gtcagcatcg ccgccatatt ctgcgcaata ccatcaaagg tctgcgtggc tgcactttta
     1741 acctgcgaca tactgtccgt ggcgctctct tcccactcac tccagccaga cctgaggcct
     1801 gccatccagc tcccgcgaag ctggtcttca gccgcccagg tctttttctg ctctgacatg
     1861 acgttattca gcgccagcga attatcgcca tactgttcct tcaggcgctg ttctgtggct
     1921 tcccgcgctg cctgccggtc agtcagcccc cggcttttcg catcaatggc ggcccgtttt
     1981 gcccgttgct gctgtgcgaa tttatccgcc tgctgcgcca gcgcgttcag gcgctcctga
     2041 tacgtaacct tgtcgccaag tgcagccagc tggcgtttgt actccagcgt ctcatcttta
     2101 tgcgccagca gggatttctc ctgtgcggac agctggcgac gttgcgccgc ctcctccagt
     2161 accgcgaact gactctccgc cttccacaaa tcccggcgct gctggctgat tttctcattt
     2221 gctccggcat gcttctccag cgtccggagt tcagcctgaa gcgtcagcag ggcagcatga
     2281 gcactgtctt cctgacgatc gcccgcagac actttcacgc cggactgttt cggctttttc
     2341 agcgtcgctt cataatcctt tttcgccgcc gccatcagcg tgttgtaatc tgcccgcagg
     2401 attttcccgt ctttcagtgc cttgttcagt tcttcctgac gggcggtata tttctccagc
     2461 ggcgtctgca ggcgttcgta agccttctgc gcctcttcgg tatatttcag ccgtgatgcc
     2521 tcagactcgg cccagtcctt tgctgccatc tctctggcct tttcaagatc ggcctgcaac
     2581 gtggcggctg aaagcccaag ttgcgcattc gctctgtcct cccatgctcc ccggagattg
     2641 gcaagaaatg ctgaggtttt accgcgccgg tggcttcggc tctgatacca ctgccatttt
     2701 ttgtccgcct catcaaaagc cttttctgct ttctccagca ttccctgggc agtgtccggg
     2761 cgaccaatat ccagcaccga atcccacatg gatttgaatg cccgtgctgt cctgtctgcc
     2821 caggtctcca gcgtgcccat gttctctttc aggcggcggg tctggtcatc aaaccctttc
     2881 gttgcggcct cgttcgccgc ctgcaatgcc ccggcttcat ctccggaacg ctgcaactga
     2941 gcaacatacg caatctgctc cgccgtcacg ttatggaact gacgtgccat cgctgtcagt
     3001 cccgacgtcg ggtctgtggt cagcttcccg aaggcttcag cgaccttgtc cacctccacg
     3061 ccggatgcag aggagaaacg cgccacactc tggctgatcg cctcaaactg ctcaccacca
     3121 cgcacaccgg cattcaccag cgccgtcagt gactcgctgg tctggttaaa cgtcagccct
     3181 gccgcctgcc cggctctgga cagggccaga atacgatctg ccgtcagtcc cgcctgattg
     3241 ccggaaagga ccagcgtttt gttgaaatcg gacagggttg agttgccctg ataccaggca
     3301 tacgccagcg caccggtcgc caccgccagc gaggtggccc ccaccatcgg cagggtgatc
     3361 gcaccggcaa gccccctgaa catggggatc atcccgccga aggagtcctt aacctgacca
     3421 ccctgttgca gcaggatcag ccacggactt tgcccgcctg caagctgcgt ggccacgtcg
     3481 gtgaactgtg caggcagcat acgcatggcg gctttatact gcccgacgga aatccccgct
     3541 ttctgtgcag ccagcgcctg tcggctcagc
//
LOCUS       DAZKHK010000266         3564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKHK010000266 DAZKHK010000000
VERSION     DAZKHK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.268"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..343
                     /locus_tag="ACHOS5_005143"
     CDS             <1..343
                     /locus_tag="ACHOS5_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9779796.1"
                     /translation="LSEEGNDMLMSFFLQTDATNLNAMYHKKQNAKLDTFGVFDNGWH
                     TLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGSADKLQLTGISKAATYTLLIDSV
                     KVEVNNADAAA"
     gene            494..676
                     /locus_tag="ACHOS5_005144"
     CDS             494..676
                     /locus_tag="ACHOS5_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9779797.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            714..959
                     /locus_tag="ACHOS5_005145"
     CDS             714..959
                     /locus_tag="ACHOS5_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9779798.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            1036..1251
                     /locus_tag="ACHOS5_005146"
     CDS             1036..1251
                     /locus_tag="ACHOS5_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9779799.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1255..1482
                     /locus_tag="ACHOS5_005147"
                     /pseudo
     CDS             1255..1482
                     /locus_tag="ACHOS5_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            2558..3091
                     /locus_tag="ACHOS5_005148"
     CDS             2558..3091
                     /locus_tag="ACHOS5_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9779800.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            3389..>3564
                     /locus_tag="ACHOS5_005149"
     CDS             3389..>3564
                     /locus_tag="ACHOS5_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9779801.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     EQANATITDMQVRQR"
BASE COUNT          971 a          771 c          984 g          838 t
ORIGIN      
        1 gctgtcagag gaaggcaacg acatgctgat gtccttcttc ctgcagacgg atgcgacgaa
       61 cctgaacgcg atgtaccaca agaagcagaa cgcgaagctg gatacgttcg gggtctttga
      121 taacggatgg cacacactgg cttttgagtt tgccggaaac aacagcattc aggtgacgcc
      181 ggtactggat gagaaacggg gtacgccgtt cacactggtg aaatctccgg catcagggtc
      241 ggcggacaaa ctgcaactga caggcatatc aaaggcggcg acatatacgc tgcttattga
      301 cagtgtgaag gtggaagtga acaacgcgga tgccgcggca tgataaaaaa ggccgccagc
      361 aatgaaggaa agggatgaac tttgtccact acatgaatgc tggcggctgg taaaacgacc
      421 atgatgacac cgaagtctgc ggtgtttttc tacaaaaata agtaaatcat gtcaactatc
      481 acggtgagaa accatgacat ttctgaacca gttaatgctg tacttctgta cggcggtctg
      541 tgtgctgtat cttctttcgg gtgggtacag ggctgtgcgc gatttctggc gcaggcagat
      601 tgataaaagg gccgcagaga aaatcagcgc cagtcagtca gccggaagca aacccgaaga
      661 gccgctcatt ccgtagtcac tttcttaaca acacctttca acgagaaaat cctatgtcag
      721 aaataaaatc gctggtcact gctgaagcag tgaaggaagt cctgcgctct gaagaagtca
      781 gaagcgcact gaaacagaaa ctccgccaga accttgagtc gcgtcttgat gcagaagtgg
      841 atgccattct ggatgaactg ctgggcgcac cggcagttcc tgagccggaa ggcattgcgg
      901 gtgacgggag tgcttcagat ggcggtgaac ccacacccga cagcgacatg atgatgtaag
      961 catgcgcaag ggaccatcgg tgtgtgtcgg tggtctttat attgttgtga gcttccggat
     1021 tgcgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc atacaccacg
     1081 tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc cccgtctcag
     1141 tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacata cctgactaac
     1201 ctgtatttca agattaaaga agaccgtcgt aaggcggcgc ggggagagta gacgatgaat
     1261 aaacaatacg aactggttgt taaagggata aataattacc cggataagat tactgttact
     1321 gtggcactgg aaattgccgg gcagccgtcg ttgttgtcgc catatgtggc gattagtctt
     1381 gaccgtactg aaggtgccaa gctggaattt tacgaagctg aggcgaaaaa gaaggcgaag
     1441 cagtttttca tggatattgc tgccgcttta tgtgaagggg atgaacagtc acgggaaaag
     1501 cgcctctgtt cagaagagcg gtataccata caaattaaca atgcatataa cacgatacag
     1561 tcagaaaaag atgatatcga atcacgcatt gaaaaactgg agaacagcgt cgttgaactg
     1621 aataaaaaac tgtcggtgca gatcccttcc gaaggtgaaa aaaaacgccg tgatgagcag
     1681 ttcgcggcgt tttacgatta ttgccggaaa gtgatgagca gaaatctcgc agagtgtttc
     1741 cgtattcaaa aaaacagaga gcgctctgca aatagtgaga ttctggataa ttttttaaag
     1801 gggacgcatc tcgccagtga ttgtttcatt gctcattctt ttggtggctt tcatgaggcc
     1861 atcaaacatg atgttgagag aagtccaggt atcctctgtt ccattcccgt aagcatcgaa
     1921 atagacacaa atacgataac cggtgaactc gttaccgccg gagtcggtat attccgtcat
     1981 gaggggaacg atgccaaagg ttcctttaca aaaatcgggc cgttctttct gaaggtagag
     2041 cgatagtttg ttaagtaccc atccgacagc tcgctcattt tggtttattt tcgtatcgcg
     2101 aaacaaaaat tcgaagcggc cagatgacgc atgggtgcaa ccaaataccg gggcgtattc
     2161 atcaaccttt ggtgttcctg aaaacatgca gtccgtgctc tcgaatgcag atgaattgct
     2221 gttgatccac ttaacaaaag aaaaaaaggt ctgaatcgct tgtctgtctg aatattcaac
     2281 aaaatcctca aggtgtgtct cgatcagttc cgggtgttta cgaaagtcat aatattgacc
     2341 gggacgatat ttatgagtgg ttgttttatc ccatggttgt gctctgcgtc cctgtaaacg
     2401 gaggtctgcc gctggataga taagcataag taatctcctg gtggaatgta atccacgatc
     2461 atccggggaa attaagaacc cgccagtgcc caccactggt ggggtgaagg cttaacatat
     2521 ccagggattc ggaaccgata aatcctgata aatatccatg aacaccaaaa tcaaatacgg
     2581 cctgtcggct gccgttctgg cgctgattgc cgcaggtgcg cctgcgcctg aaatcctcga
     2641 ccagtttctg gatgaaaaag aaggcaacca caccacggca taccgtgatg gcgcgggtat
     2701 ctggaccatc tgccggggtg ccatcatggt ggatggtaag cctgtgattc ctggcatgaa
     2761 gctgtcgaag gggaaatgcg accgggttaa cgccattgag cgtgataagg cgctggcatg
     2821 ggtggagaaa aacatcaaag tgccactgac cgaaccccag aaagcgggga tcgcgtcatt
     2881 ctgtccttac aacatcggcc caggtaagtg ttttccgtcg acgttttata aacgaattaa
     2941 tgcaggcgat cgcaggggag cgtgtgaagc gattcgctgg tggattaagg acggtggcag
     3001 agactgccgt atccgctcaa ataattgcta cggtcaggta tcccgtcgcg accaggagag
     3061 tgcgctggca tgctggggta tcgacaggta gtagaatatt ttgctgaaaa atgacgttga
     3121 ccaacgcgga cggataacac gaaatcctgc gaactggcaa aacctaagtg aataaaagta
     3181 aaaaccccgt ttgttggcag caagcggggt tttgtgtttc ctgactccgg aaaagtcaaa
     3241 ggagaaagtg tgtttgattt tagcaaactg attcgggaga ttcgagtgat ggctgaaaaa
     3301 ttatccacct ggaagttcat ccttatctgg ctggtgtttg tgattatggc ctccggttat
     3361 ttcatcggtc agatacgctg gtggtgaaat gaaccgcgtt ctgtgtgtgg tcatcattgc
     3421 cttgctggtg gcctgtggtg cgcttagtct ggggctgaat cattaccgcg ataacgccat
     3481 cacctacaaa gcgcagcgcg ataaaaaagc cagagagctg gaacaggcga atgccaccat
     3541 tactgacatg caggtgcgcc agcg
//
LOCUS       DAZKHK010000267         3546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKHK010000267 DAZKHK010000000
VERSION     DAZKHK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.269"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..1566
                     /locus_tag="ACHOS5_005150"
     CDS             52..1566
                     /locus_tag="ACHOS5_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9779802.1"
                     /translation="MTEENRRIKAITRFSYGSGNLIGSGALAISGAWLLYFYTTFCGL
                     TVFQATVIFSIATYLDVILNPLMGFITDNFYQTKLGRRFGRRRFFILIAIPCMIIYPL
                     LWVNGMSFWYYLSTYIIFEVVYTMIMIPYNTLPVEMTKDFNQRTYLTGSKAMFGKVAN
                     FLAAAIPGIYFYFFNKDSATPFFLTGLTYAIIMITSLIMLYFNSWERPTDDVVDESTH
                     GFMEGFNKLFTDLFSTFKLKTFRTHLGMYLFGFGAEWLFTATFTYFIVFNLEQPRTFV
                     SEMNSLSSICQFFSTAFFMMWCAKRGFKRPFIIAILIVVSSVIGYVGVYYLNIPHLTW
                     LIIGITIWFGLGTGGVYYIPWSVYVFLADVDEVLTGRRREGLYAGAMVMAGKLMRATV
                     VFCLGVVLSLFGFESGIHHQSASAVNAINGVMIFGVAGMALIGIYFSVKMKLDKASHA
                     EITKELERLKKGGRKEDATPEIRKLVKQLTGYEYDQCFGNNNVGFKFENKAGAF"
     gene            1568..>3546
                     /locus_tag="ACHOS5_005151"
     CDS             1568..>3546
                     /locus_tag="ACHOS5_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolygalacturonate lyase"
                     /protein_id="HFP9779803.1"
                     /translation="MNKHTLLLTVLFLNLICTPVFAQNWQVATFGQSTDLNFSSLIDS
                     AKIGRNNAWLAGNNNFLEAGKFYTLPTDFFIESRGGKIANSHDGMTVFYTIVPVTQTF
                     RLEADLTLEQIGPEVNGKSPAGQEGAGLFVRDIIGPQRQEPQSAGTEEYPQASNILMN
                     AFITQNKKNDNLVQITSIVREGVIKTWGNEGITIKKQPIIENINFTQKRNIHMTIERL
                     PEKFILTAFDTDRKENQSWQFSDYSGFMNQLDNNSLAIGFFAARNAKLRVKNASFKPG
                     KPLVDYKQLTSRQFSRVRHKAPELFLASPQSVVRNSTTLQFLANQAGIVSIDNDKQTK
                     QVQAGELVQFPVTLQKKHNDFTVNFNVDGNISKKAIRIEQVKSNLTDPYEIYVCSDCR
                     QGARGSKNDPVDLQTAVKFVAPGGNIYLNDGQYHGITLDRELSGIPGKYKTISAINPH
                     KAIFINKTFNLDASYWHLKSVVFDGNVDNGNNKPAYLRIAGSYNIIEHVIARNNDDTG
                     ISISAKDKNRFFWPAHNLVLNSDSYNNLDLSGINADGFAAKLGVGPGNIFRGCIAHNN
                     ADDGWDLFNKIEDGPNASVTIENSVAYENGLPYNKADILKGSIGNGFKLGGEGQPVNH
                     KVINSIAINNNMDGFTDNFNTGSLIVRNNIAMNNAR"
BASE COUNT         1070 a          616 c          733 g         1127 t
ORIGIN      
        1 atcttttcca ggagatggaa atggactttt gttgtggaag aggtatcata aatgactgaa
       61 gaaaacagaa gaataaaagc cataactcgt ttttcatatg gtagtggaaa tcttatcggc
      121 agtggagctc tggctattag tggagcatgg ttactttatt tctacaccac attttgtgga
      181 ttgacagttt ttcaggccac tgtaatattt tccattgcta cttatctgga tgtcatcctg
      241 aatccactaa tggggtttat tacggataat ttttatcaga caaaactcgg tcgtcgcttt
      301 ggtcgtcgtc gcttcttcat attaattgcc attccatgta tgattatcta tccattgctt
      361 tgggtaaatg gtatgagttt ttggtattat ctttctactt atatcatttt tgaagtggta
      421 tatactatga ttatgatacc gtataatacc ttacctgttg aaatgacaaa ggatttcaat
      481 caaaggacct atttgacagg ttctaaagct atgtttggta aagtagctaa tttcttagca
      541 gccgctattc ctggtatcta tttttacttc ttcaataaag attcagccac cccatttttc
      601 ctgaccggtt taacttatgc tattattatg atcacttcat taataatgct ctatttcaac
      661 agttgggaac gtccaactga tgatgtagtt gatgaatcaa cgcatggttt tatggagggc
      721 tttaataaac tttttaccga tcttttctca acctttaagc taaaaacctt cagaacccat
      781 cttggtatgt atctatttgg tttcggcgct gaatggctat ttacagcgac atttacctat
      841 ttcattgttt tcaaccttga acaaccgaga acatttgttt ctgagatgaa ctcattaagc
      901 agtatttgtc agttcttctc tacagcattc tttatgatgt ggtgtgccaa acgaggattt
      961 aaacgcccat ttatcattgc tatcttaatt gttgtatctt cagtgattgg ttatgttggt
     1021 gtgtactatc ttaatattcc ccatctgacc tggctcatta ttggcatcac tatctggttt
     1081 ggtctgggga cggggggggt atattacatt ccgtggagtg tatatgtatt ccttgccgat
     1141 gttgatgagg tcttaactgg tcgtcgtcgt gaaggtcttt atgctggtgc aatggttatg
     1201 gccggtaagt taatgagagc cactgtagta ttttgtctgg gggttgtcct cagtctgttt
     1261 ggttttgagt caggcattca tcatcaatca gccagtgcag taaatgcaat taatggcgtc
     1321 atgatatttg gtgtggctgg tatggcgtta ataggtatct atttctctgt aaaaatgaaa
     1381 ttagataaag catctcacgc cgaaataact aaagaattag agcgtctgaa aaaaggtggc
     1441 agaaaagaag atgctacacc ggaaattcgt aagcttgtta agcaactgac tggttatgaa
     1501 tatgaccagt gcttcggtaa taacaatgtt ggctttaaat ttgaaaacaa agcaggggca
     1561 ttctagaatg aataagcaca cactattact gactgttctt tttctgaatt tgatttgtac
     1621 tcccgttttt gctcaaaact ggcaggtggc gacgtttggt cagtctacgg atctcaactt
     1681 ttcatcgctg atagattcgg ccaagatcgg acggaataat gcctggcttg caggaaacaa
     1741 taattttctt gaagctggaa aattttacac tttaccaaca gattttttta ttgaaagccg
     1801 tgggggaaaa attgctaact cccatgacgg tatgaccgtc ttttatacta ttgttccggt
     1861 tactcagaca ttccgactgg aggctgattt gacattagaa cagattggtc cggaggtgaa
     1921 tggaaaatca ccagcgggac aggagggagc tggattgttt gtcagagata ttatcggtcc
     1981 tcagcgacag gaacctcagt cagctggaac agaagaatat ccccaggcct ctaatatatt
     2041 gatgaatgcc tttattacac agaataaaaa gaatgataac ttagtacaga ttacttcaat
     2101 tgttcgtgaa ggagtaataa aaacatgggg taatgaaggt attacaatta agaaacagcc
     2161 gatcattgag aatataaact ttacgcaaaa aagaaatatt catatgacga tcgagcgact
     2221 accagagaag ttcatcctga ccgcttttga taccgatcgt aaagaaaatc agtcatggca
     2281 attttctgat tactcaggct ttatgaatca actggataat aatagtttag ctattggttt
     2341 ttttgccgca cgaaatgcga aactaagggt gaaaaatgca tcatttaaac cgggcaagcc
     2401 actggttgat tacaaacaat taacttcacg tcaattcagt cgtgtccggc ataaagcccc
     2461 tgaacttttt cttgcttcac ctcaatccgt tgtaagaaac tcaacaactc ttcaattttt
     2521 ggccaatcag gctggaatag tcagtattga taatgataag cagactaagc aggtgcaggc
     2581 gggtgaactg gtacagttcc cagttacttt gcaaaaaaaa cataatgact tcaccgtcaa
     2641 ctttaacgtt gatgggaata tatcaaaaaa agctatacgc atagagcagg ttaaatcaaa
     2701 cctgactgat ccttatgaga tttacgtatg tagtgattgt cgacaggggg ccagaggcag
     2761 caaaaatgac cctgtagatt tacagacagc cgtaaaattt gtcgcacccg gcggtaatat
     2821 ataccttaac gatggtcaat atcatggaat taccttagat cgggaattaa gtggaatacc
     2881 tggcaagtat aaaacaattt ctgccattaa tccacataaa gccattttta taaacaagac
     2941 attcaatctg gatgcaagtt actggcatct aaaatccgtg gtctttgacg gcaatgtgga
     3001 taatggaaat aataaaccag catatttgcg tatagctggt agctataata ttattgagca
     3061 tgtgatagcc agaaataatg atgatacggg aatttctatt tcagcgaaag ataaaaaccg
     3121 ttttttctgg ccagctcata acttagtttt aaactcagat tcatataata atcttgattt
     3181 atccgggatt aatgccgatg gttttgctgc aaaattaggt gtcggaccgg gaaacatttt
     3241 tcgaggatgc attgcacata ataatgcaga tgatggttgg gacctattta acaaaattga
     3301 agatggtcca aatgcatctg ttactattga gaattctgta gcctatgaaa atggcctgcc
     3361 atacaataaa gcggatatcc taaaagggag tattggcaat ggctttaaat taggtggtga
     3421 aggtcaacca gtaaatcaca aagttattaa ttccattgct attaataata atatggatgg
     3481 attcactgat aattttaata ctgggtcatt gatagttaga aataatatag caatgaacaa
     3541 tgcacg
//
LOCUS       DAZKHK010000268         3521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKHK010000268 DAZKHK010000000
VERSION     DAZKHK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.270"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..248)
                     /locus_tag="ACHOS5_005152"
     CDS             complement(<1..248)
                     /locus_tag="ACHOS5_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779804.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATG"
     gene            complement(245..1870)
                     /locus_tag="ACHOS5_005153"
     CDS             complement(245..1870)
                     /locus_tag="ACHOS5_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779805.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSKIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNAALATEQIISPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGA
                     LPSTIRWHSKIFSLPERTSFSCISVKSPAPERVGITVMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(2210..2437)
                     /locus_tag="ACHOS5_005154"
     CDS             complement(2210..2437)
                     /locus_tag="ACHOS5_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779806.1"
                     /translation="MYKCVLAVLILLSGCKSLPTPLCHGKAYLGGEETIIPIYGIKKS
                     GKYKLYHAGHHYNWRWVGAGAFDSTTCSQIM"
     gene            complement(2450..2995)
                     /locus_tag="ACHOS5_005155"
     CDS             complement(2450..2995)
                     /locus_tag="ACHOS5_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779807.1"
                     /translation="MGVASGWVGSSAVSVTGERSMKSAGAKLKLSTPFYMSQMVGKSV
                     QDFSITVGRSNFVVLVQATNIQIVPVNNNYGGYSNNSTTTGAGSAVSSRVQTQNPAGS
                     INGSLLGCSITHLSQTQDSTKMYLGLTNGPNQNFTLSFDGTNISFTPSSFANNTRHYS
                     AIVSKNWLFNQNGKTISVYKV"
     gene            complement(3078..>3521)
                     /locus_tag="ACHOS5_005156"
     CDS             complement(3078..>3521)
                     /locus_tag="ACHOS5_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFP9779808.1"
                     /translation="QGPKGETGAPGQGTELLTTANTWTQAQTFNGGINGNLTVTGNGS
                     FNDVQIRSDKRNKRNAIRIDNCLEKLDLLTGYLYEIQNADGSWQQSVGLFAQDALKAQ
                     PELVTSDTDIISGEERFRLNYNGVIALLVEGIKNLRKEISDLKEK"
BASE COUNT          936 a          917 c          715 g          953 t
ORIGIN      
        1 ccggtcgcct taaaattcag cggttttgtg ggaaaatcaa cagtcattaa agtactgtca
       61 atctcaatac ccggattcag tgcatatgag gcaccacccg atgttcgacg ccgggcgagt
      121 ttaagaccaa ccagttcctc acgggtcaca aatgcgtgac gtccgtcacc acgctgcccg
      181 gtgccaattt ccatattctc cacaacagtg gataaatcct tccccgcacg ccacggtttt
      241 ctggtcatac cggcatctcc gacattgatg tactcagggt tattcgttcc acctgcccga
      301 atccggatac catcacctgc cagttttgcc cggttgctgc cggaagtctc accacacttc
      361 ccttaaacgt acccggcgca aaatgaatca caggaacatc atcagccata acggtgatcc
      421 ccacccgctc aggtgccgga gatttcacac tgatacagga aaaagaggtt ctttcaggta
      481 atgaaaaaat ttttgaatgc caccttatcg tggagggcag agcccccccg gcaagcactg
      541 acattttgtc tcctgtcacc acgcgcatca tatctttcgc gagatcaacc catgcgcagt
      601 caaacggtgt actgagataa cggatatcca tgttcaccgg actgaataca aacacatcct
      661 gcttaccatc cggtttcgtg taacaggcaa tgtattcacc acgccaggga taagccacaa
      721 tggaggccgg attaaactga ctctgccact gttccggtga aataatctgt tccgtcgcca
      781 gcgcggcatt accgtttgca tcaacagaca ccaggccatt tgttcctgca tacagcacaa
      841 aaccctccat cgcaaccata ctccgcctgc tcagacacgc ctgcattgaa gggattttgg
      901 aaccagaaat tgtggacggt gataccccac tgaacaaata aggctccccc tttgtcgcca
      961 ccaccagtga cgttcccagc ggacagatag ctacaatatc ttctgccgtc gtgtgacgat
     1021 tcacttccgg ccatgcatac ggcagatacg cttccgaaaa catcacttca ttaccggcaa
     1081 acccggcggc aataccgtta gccatcaggc aaaggcctgt catattctct ggcggcggca
     1141 ggtaatccca tgtcgccagg gaaggcccaa ggtttttcgc cggtattttg tccgtgtaac
     1201 tgagcacgga tgcatccagt tcagccacaa gtaaaaaatc cgcctcccct ccacctgatg
     1261 cagagcgata aatccggcga cgtttaatac tggcattctg caatggcacc ggagccagcg
     1321 tcagttgtac cgcagttccc ggagtacgga gtgttacctc cagagacgcc ggacctggag
     1381 gaccttcttc accataatct gagacaaagg tttccgtata aaaccgggtt tcatcatcat
     1441 tcgggttatc gtcagaaaca tcaccgccct gctgaacagt acagacagga gctgtcgtcg
     1501 gcgcggggat ccccagacga tacgatgatg tcgggtgatt cccgtcccct tttgtggcaa
     1561 tggtcgcatc cgtcacttta ggaaaacgcc cgtcagtgta gtaaatacgc ccgtgggggt
     1621 cctgagcgat cggactgcgg atcacatcca ccacatccgg ccatgcaaac cagaaatcgt
     1681 cacggtaatg aaaaattgtt tttggcttaa ttgtgaatgt tttctcaacc ccggatatct
     1741 gacgttctgg tgtaataaca ccaaaccgga aatggcagtc ctccgccagt acagcggaat
     1801 gctcgggcag catggatgtc acaacgcgcg gcatcatccc acgcatcgtg gtgatatcga
     1861 tatagggcat agaaattcct ttgactgctt caggcttaaa aatcagggga tttatgatgg
     1921 aattcgctaa aatcgcgatc ggtatcactg caacatagtg ttgctatgat attctggaca
     1981 agacgtaaca actcactaat tgtttcaaac ttctttcttc tggtcgggga gcatgttgct
     2041 ccccttttta ttgcatcatt acggcagcca ctgcacagag cgttaccact gcacccagcc
     2101 acacaaaaag ctcctgctgt gtagcgcttc tttacgtatt tcagatttgc ctgtttcatg
     2161 acaatttatc aattagcatt agctaaacat tttatacata gtatacggtt tacattattt
     2221 gagagcaggt tgtgctgtca aatgctccag caccaaccca acgccagttg tagtgatgcc
     2281 ctgcatgata caatttatac ttcccgcttt tcttaatccc ataaattggt attatagttt
     2341 cttccccacc taaatatgct ttaccatggc aaagcggagt tggcaaagat ttgcaaccag
     2401 ataataatat caatacagct aaaacacatt tgtacattat caactccttc taaaccttat
     2461 aaacagatat agtttttccg ttttgattaa agagccagtt tttagaaact attgcactat
     2521 aatgccgagt attgtttgca aatgatgatg gtgtgaatga gatattggtt ccatcaaatg
     2581 atagagtaaa gttttgatta ggcccgttcg ttaagcccag atacatcttg gtagagtctt
     2641 gcgtttgaga aagatgcgta attgaacaac ctaacaacga accatttatt gaaccagccg
     2701 gattttgtgt ttgcacacgg ctggaaacgg cagagccagc acctgtcgtt gttgagttat
     2761 tagaataccc tccatagtta ttgtttacag gaacaatttg tatatttgtt gcctgaacta
     2821 aaacaacaaa atttgaacga cctacagtga tagaaaagtc ctgtacactt ttcccaacca
     2881 tttgcgacat ataaaacggt gtacttagct taagtttggc acctgccgat ttcattgacc
     2941 tttcaccagt tacactaacg gcagaggagc caacccatcc ggaagcaacc cccatttaac
     3001 atcctcctgt cattcggacg ataacaccaa ctatcttcta cctcagacta atggttctct
     3061 gttctctgat tgttttatta tttctcttta aggtcgctaa tctctttacg cagatttttt
     3121 attccttcaa ctaacagtgc aataacacca ttataattta atctaaatct ctcttcacca
     3181 gatatgatat ctgtatcaga tgtaactaac tctggttgag cctttaacgc atcctgtgca
     3241 aataatccca cagattgttg ccaactgcca tcagcattct gtatttcata caaataaccc
     3301 gttagcaggt caagcttctc cagacaatta tctattcgta ttgcatttcg cttgttacgt
     3361 ttatccgaac ggatctgaac atcgttaaat gagccgtttc cggtcaccgt caaattgcca
     3421 ttaataccac cattaaaagt ttgtgcctga gtccatgtat tggcagtagt aagcagttct
     3481 gttccttgcc cgggggctcc agtttctccc ttcggtccct g
//
LOCUS       DAZKHK010000269         3488 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKHK010000269 DAZKHK010000000
VERSION     DAZKHK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.271"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..551)
                     /locus_tag="ACHOS5_005157"
     CDS             complement(<1..551)
                     /locus_tag="ACHOS5_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9779809.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSG"
     gene            complement(622..>3488)
                     /locus_tag="ACHOS5_005158"
     CDS             complement(622..>3488)
                     /locus_tag="ACHOS5_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9779810.1"
                     /translation="YPNTALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQ
                     YSGIWDGTFKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVP
                     DGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTY
                     NRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRN
                     VTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYA
                     GISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGSPVSVEVQSVTDGVKVK
                     VSRVPDGVAGYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGA
                     HFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTAD
                     DGSERLVSTARTTETTYRFRQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSR
                     IELTPGYFQITATPHLAVYDPTVQFEFWFSEKRITDIRKVETTARYLGTALYWIAASI
                     NIKPGHDYYFYVRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQI
                     DNGQLAPDLTEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAV
                     KLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQI
                     FMNEVFLKRLTAPTITSGGNPPAFSLTSDGRLTAKNADISGSVNANSGTLNNVTINQN
                     CTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDHNFDRQIIIP
                     PIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPS
                     GRGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          719 a         1081 c          861 g          827 t
ORIGIN      
        1 ccactgccgg agccttcata agcaatatca atggccacgg attcggtcgg gttaaactgc
       61 acgccagccc cccacgccag agacgtgttg ctgtggcgac cgtcatcact tccggtcagc
      121 acatcgtgct ttttcccctt gttgtcagtt acgcgaagat aatccccgga gaaagtcgac
      181 acacggctgt aagctacacc cgccatcgca tacgcgctga accattcatt cacgcgcaca
      241 gacggccccg ccattacgct gaaccagcgg ttacgaacgg aatcttcatg ccagcgggta
      301 tcgctgtaac gggtcagctg gcgattcttg tctcctgcat agctgaatga cgtcaccagc
      361 cccagtgtgt ccgtaaactc ataacggtat ttcacgttaa tcccgttcag atcatcactg
      421 ccggggacgt tcgtcgaggc atgaagatac cccgcgctca gcgtggactg atgttcagac
      481 gcccatgcag gcgcaccgga tacggccaga caaatggctg cggacaaaat tgctgcacaa
      541 actttacgca taattacctc tcgcttttct gcaataaaaa aggcgtcatt cctgacgccc
      601 tttattgggg ttataaatat ttcaacgaat actgatgccg gaagcggctt ttttggtcac
      661 aatcaccgta cagtcggtga tattgcctgc cccctgattg cctttctgga aaatcttaaa
      721 ctccagagtg acgcttcccc tgccactcgg catatcaata actgcactgt aactaccggg
      781 aatggcccct ttagtttctc tggatgcgat taatacgccg tttttgcgaa cttcaaaacc
      841 ataacccgtg tatcgcgtgc ctcctgggtt atttccgctc cccggatcgt catacgctat
      901 accgttaaaa ataatgggcg gaataataat ctggcggtca aagttatgat catcgctgat
      961 ggtgactgta accgtaccgt ttggtgtttc cgtgttaccc cacgtaccga cttttttcgg
     1021 gaaggctttt gatacagctt taacgaaatc tcctctgacc tgggtcgcct ccagcatgcc
     1081 cttaatcgta cagttctggt taatcgtgac attgttgagc gttcctgagt tcgcattcac
     1141 actgccactg atatccgcat ttttcgccgt cagtctcccg tctgatgtca gggaaaatgc
     1201 cggaggatta ccgccgctgg taatggtggg ggccgtcagg cgtttcagga acacttcatt
     1261 catgaatatc tgatcgccct gaccaacaaa catcggcttt gtgttgccat tcgcaggatt
     1321 aatcatcgca atcctgtctg ctgccagcag cacctgactc tgcatgccgt caggggtgtt
     1381 ctcaataccg gcaccgatac ccgcaatata aaggcgtccg tcctgcatct gctgcagctt
     1441 cactgcccac atgctgttca ggttattatt tgtatcaacc tgaaccttct gtatctgctg
     1501 gatcgctgca ctctggtctt ccagtttctt attgacggtc tgtgttattt cattgctgac
     1561 atccgttatg gacgtcctga tttcagtcag gtcaggcgca agctgaccgt tatcaatctg
     1621 cgtccacagc tcctgagcca gatgggtttt ccctatctcg cctttgaaaa aatccagata
     1681 gcctgatgca tcatcactcg gctgaccgac agcctccacg aatgccgatt tgccaacggt
     1741 gttcacactg cgaacgtaaa aataataatc atggcccggt ttgatattga tactggcggc
     1801 tatccagtac agcgccgtac caagatagcg cgctgtggtt tcaaccttcc tgatatcggt
     1861 aatccgcttt tccgagaacc agaactcaaa ctgtaccgtc gggtcataaa cggcaagatg
     1921 cggcgtggcg gttatctgaa aatagcccgg cgtcagctca atcctcgacg gtgctgccgg
     1981 tgcggcaatc cggaacgata ccgacgccgg atcgccctgc tgcccccacg catttaccgc
     2041 ccggactgtc agcctgtagt tccccagcgc cagttgcctg aagcggtatg tggtttccgt
     2101 cgtccgggcc gtgctgacca gccgctcact gccgtcgtcc gctgttacgg tcagacggag
     2161 caggaagctc acccccttca ccaccttcgg cgtgtcccag cgcgccagca cctgatattc
     2221 cccgctgtct gcggtgactt cggcagtcag gtgctgcacc gctggcggcg tgacaccatt
     2281 caccgtgccg ctctggtcgc cgtcaaagtg cgccccgtta tccacgatgg cctctttttc
     2341 cggtacatgc tgcacggcgg tgatggcata cgtgccgtca tcgttctcac ggatactcac
     2401 gcagcggaac aggcgctggc gcagcgtcgg caacttcagc ccccatacgc tgtatccggc
     2461 aacgccgtca ggaacacggc tcactttcac cttcacgccg tcggtgacgg actggacctc
     2521 cacgctgacc ggactcccct gcccgtcaac caggcttatc agcgtggtac cggaagatgg
     2581 cagcgtgatt tcacggtcga gcgtcagcgt ccgggtctgg ctgttcaccg ccagcacgcg
     2641 cccgccggtg ctgataccgg catagtcatc atcgcagatt tcaatgacat cgcccggtac
     2701 atggcgaagc ccttcggcac ccacgctgaa gtccacggtc tgcgtctcca gcagctccgt
     2761 tttaatcagc cacagcccgg cgcggtgtgc ctgcccccgg ctggtacagc caaaagcatc
     2821 catcttcgtg acgttacgac cgtaacgggc aatggcctgc gtgtcctcca caagctctgt
     2881 cgccgtctcc cagccgttat tcgggtcaat ccagttcacc tcaacggcat tatggcggtc
     2941 cttcagggcg ctgaagctgt agcggaacgg cgcaccatca tccggcatca ccacattact
     3001 gcggttatag gtccacacct tatccgacgg tcggtcctgc acgaacgtca gcgtctgccc
     3061 gttccatacc ggcatacagc gcatcgccga gcagaaatca ctgagaacat cccacgcctt
     3121 gcgctgtgtg gtcaggtagg cattacaggt gatgcgcggc tccgtgccac caaagccatc
     3181 cggcaccgac tggtcgcaat tctggccgat gacatacagc gcccatttat ccacatccgc
     3241 cgcaccaaga cgtttcccca tgccgtagcg cggatgggtc agcatatccc acagacacca
     3301 ggccatgttg ttgctgtatg ccggtttaaa cgttccgtcc cagataccgc tgtattgccg
     3361 cgtctgcggg ttatagttcg acggcacctg cagaatgcgc ccgcgcagat gataattacg
     3421 gctcacctgc tggctgccga actgctccga atccacctgc acgccgacca gtgccgtgtt
     3481 cgggtagc
//
LOCUS       DAZKHK010000270         3434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKHK010000270 DAZKHK010000000
VERSION     DAZKHK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.272"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(156..1049)
                     /locus_tag="ACHOS5_005159"
     CDS             complement(156..1049)
                     /locus_tag="ACHOS5_005159"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9779811.1"
                     /translation="MSALVLCGNSGVILLSVTDHQHIDSAIPGRYTVQAPYKTGAGRG
                     NPEFTKAHNRALAVFLCHEQHYAQIMVGRAGPTSVGPGSLVTGISTPVRLTTNKVVES
                     LGGELLKITKEAAIMATIPTLTQPEIAIVDGQVVTSSLAVANFFSKRHDDVLKKIRTL
                     ECSASFTARNFSVSDYTDCTGRKLHCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFN
                     QMEKQLSKPAVPSDVAHNASVLCSYISSIHQVWLQQLYPMLAKAESPLAVSLYDYIND
                     ASALACLINLSLNPSEVRGRK"
     gene            complement(979..1158)
                     /locus_tag="ACHOS5_005160"
     CDS             complement(979..1158)
                     /locus_tag="ACHOS5_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9779812.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECDGPPTH"
     gene            complement(1304..1855)
                     /gene="ymfL"
                     /locus_tag="ACHOS5_005161"
     CDS             complement(1304..1855)
                     /gene="ymfL"
                     /locus_tag="ACHOS5_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP9779813.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(1848..2108)
                     /locus_tag="ACHOS5_005162"
     CDS             complement(1848..2108)
                     /locus_tag="ACHOS5_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779814.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEISRG"
     gene            2206..2898
                     /locus_tag="ACHOS5_005163"
     CDS             2206..2898
                     /locus_tag="ACHOS5_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9779815.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDFKSNTVCKNSISEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
BASE COUNT          947 a          821 c          813 g          853 t
ORIGIN      
        1 gcggtaatta cggcccagcg gttcacaaac caccttctga cattcacggt caaccaggct
       61 aacacgtaga acatgccctg caggcgtggt gtaccactga cccggacgag gacaacggaa
      121 agtctgattg gtaaaacgtt tgaaaatatt ccggatcatt tgcgccccct tacctctgaa
      181 gggttcagcg acaaatttat gaggcaggcc agcgccgaag catcattaat ataatcatat
      241 aagctaacag ccagcggaga ttcggctttt gccaacatag gataaagctg ctgcagccag
      301 acctgatgaa ttgatgaaat gtaggaacag agaacgctgg cgttatgtgc aacgtcgctc
      361 ggtacagcgg gctttgaaag ctgtttctcc atctggttaa aggcattgat atatgcctct
      421 ttgaactggg cagcacgttt acccgtgaaa cccatagcaa gaaacgcaaa accgtcgcgt
      481 gtgatttggt agcaatgaag tttgcgacct gtgcaatcag tgtaatcact caccgaaaaa
      541 ttgcgggcag tgaatgatgc tgagcattca agcgtgcgga tctttttcag tacatcgtca
      601 tgacgtttgg agaagaagtt ggcaacagcc agggatgaag taacaacctg accatcaacg
      661 atggcaattt caggttgagt gagggttggg atcgtagcca tgatggcagc ctctttggtg
      721 atttttaata actcaccacc aaggctttcc acgaccttat tggtggtgag acgtacaggg
      781 gtggaaatac cggtcaccaa agaacccggc ccaaccgaag ttggccctgc acgccccacc
      841 ataatttggg cgtaatgctg ctcatgacac aaaaaaaccg caagagcgcg gttgtgcgct
      901 ttggtgaatt ccgggtttcc acgcccggca cccgttttat aaggtgcctg aacagtgtaa
      961 cgtcccggaa tggcagaatc aatgtgttgg tggtccgtca cactcaacaa aatcacgcct
     1021 gaatttccac aaaggactaa agcactcatg cgggtagtct ttgcgaagat agataacgcg
     1081 ctgtgtttct ggttcccaac gaataacatg aacataaagc cctcttccgt cacgaaacca
     1141 gcggttaagt tcctgcacaa ctcgcccccc acagtcaggt aaagttctct gtggttactt
     1201 acagcctggt gatttggtaa tctgcattca tgccgtaaca acaggtgttc agagacgctg
     1261 accaccagct gttgcgacaa acggttattt gccgttaaac tgttcatgcg ttagtttctc
     1321 cacagacaca aaacgccacg acgcccggag ctgcacactc gcgggcgtca ctcttttctg
     1381 gagcgcagaa aattttgtag accagtgccg catgctcctg gagcttcgaa atcgacagat
     1441 acaactcatc attaattgct gtctgctcgt gtggctccac taccccatct tcgattgccg
     1501 aacgaatctg ctttgagtaa ctcccgattt gttcgatgac ttccagcagg cgctggttta
     1561 tatcggcgtt ctctacttcc tcaatttcag gaagcgatac aaacacccca ccagcagact
     1621 gtgcgacagc atccgcaatg tagtgagtgc cagcagcgcg ctgtaaaatc attgcccatc
     1681 ccagcgggaa aatctgatcg ccatctgcac gaaggcggtt gaataaagcg ttctctgtta
     1741 catccagcca ctcagcagct tcagcgtaac cccccggcaa cgccgcgata gtttttctga
     1801 cagctttcac gtaccactca ggctgttttt ccactttcca gtgatgctta cccacggctt
     1861 atctcctgtt cctgtggttt aaacccattc tggttttggc tagattgaaa acgtgccgga
     1921 taaagaatct gcatttcgct gatttcaccc ttaaaaaaat tggccagacg ttctgcaagc
     1981 tcgatagatg gaatttgttc cagtctttca atacgactca gcgtcgctgg attgacctga
     2041 acgccagcag caacatgctg caaagtaaat ccgtgcgctt tacgcacatt ccgtaatggt
     2101 gattgcatat aacctccaca tattgcgtga tgagcatatt atttcacgca aatattttgc
     2161 gcaagttgat ttgcttaacg cgcaataaag aaatgtaata aacgcatgaa cataggaaat
     2221 cgagtcagac aacttcgcca agcgaagaac atgaaaatcg ccgatctcgc tgaagcaata
     2281 ggagtggatg cggcaaatat ctcgcgcctg gaaacaggta agcagaaaca attcactgaa
     2341 caagccctga gtaatattgc caggagctta ggtgttgata ttgctgatct ctttacctca
     2401 gacttcaaaa gtaatactgt atgtaaaaac agtattagtg aggatgttgc gcaggtgaag
     2461 gatgtattcc gtattgaaat gctggatatc agtgccagtg cgggaaatgg ccttatccag
     2521 ggcggtgatg tcattgatgt gattcatgcc attgaataca gaactgataa tgctgtatcg
     2581 atgtttggcg gacggccagc aaatcacatt aaagtaatca acgttcgtgg ggacagtatg
     2641 tgtccaacca ttgagccagg agatctcatc ttcgttgatg tcagtatcaa tcagtttgat
     2701 ggagatggta tctatgtatt tggttttgat gataaaattt atgtcaaacg actgcaaatg
     2761 atacctgaca aactactggt gatttctgat aaccagattt accgtgaatg gggaattacc
     2821 agcgaaaatg aacaccggtt tatggtcttt ggaaaggtct taatcagcca gtcacaaacc
     2881 cttaagcgac acaattaacc cttacctcct catcaattag ccacccgaag gtggcttttc
     2941 attacccatc aaattgcata tctcgcaaca aaaacacttg cataatgcgc aacttcattt
     3001 tatctttctt tccagaccaa caaacaaggt actaacaaaa tttggttgta acacggcgta
     3061 tggcacatgc gtcgttagcg gtctggggac gttaaagggg acaatccact ccttgctcgg
     3121 gcaaacaaac caggtagccg gaatgtgcaa gtcaatgatg atgctgataa gacgcctaac
     3181 ctgcgtggcg gttcggtttg acacctggga agagaccagg gtgcaacgat gagggcattt
     3241 atggaaccgc gacaaagtgt ggtgccgtaa ctggctaagt gctctcagcg ttgtggtgaa
     3301 tgcgcaggct gatgcgcgaa agacattgca gctattgcgg aaaagagctg ttcggcgggg
     3361 caattaaaca cccgtgagag tctgaaataa ccgcaagccg gagatcagca ccggtcacca
     3421 caacagccac tgct
//
LOCUS       DAZKHK010000271         3410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKHK010000271 DAZKHK010000000
VERSION     DAZKHK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.273"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            107..574
                     /locus_tag="ACHOS5_005164"
     CDS             107..574
                     /locus_tag="ACHOS5_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779816.1"
                     /translation="MRLSNIFLAVVLTVTVSVAHAIPNMWGSGFTMGVTEFSIDNGKW
                     GINISCTGNPNENNVLAHTIYIYKGDKVVANSEEKNISFVIDGEEFWGVVPDGTRMND
                     NAWVSFVKAISTATGFELYINEKKVATFNPSAKNVKKVMDNNFIQSCWNTWYE"
     gene            630..1259
                     /locus_tag="ACHOS5_005165"
     CDS             630..1259
                     /locus_tag="ACHOS5_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779817.1"
                     /translation="MKHFLILVIGLLISITATAESLGQFEEDITGFPQGVWFYKEKAG
                     WKNETGYKKEYLNSLLYKAEEIANYPGNRYTIYRSKNSSIIFVADNETRTFYLLNNKD
                     ALWSDIQIGYWAENKNSCHYVFVDTQYLAQSNELDEFFRTGVLDTHGVGESDRKVKEI
                     RQKHKTHVLLRLPAQCVNDAILSRMNKSERRDLMKAQEDFKQYLARMEL"
     gene            1694..2146
                     /locus_tag="ACHOS5_005166"
     CDS             1694..2146
                     /locus_tag="ACHOS5_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779818.1"
                     /translation="MKHRHILTFFVAFLFMTIVPDSDAALVKRPEKGRAPFPEYVEFA
                     QNRNQFPCNARLRFKGLCFVEQTDPWGAASVVRIPFNSPNGKGRLNAYGVVVHAIMVG
                     DVVVGGSYSTGKNVTWNFDTFYREGKRRGKVTFYVDGKYWHYRDYPLE"
     gene            2264..2497
                     /locus_tag="ACHOS5_005167"
     CDS             2264..2497
                     /locus_tag="ACHOS5_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9779819.1"
                     /translation="MRGCTLLLFSEAPFLREQEVAVITAYRNVFIQDDPGMHFRWVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGPLRQ"
     gene            2597..>3410
                     /locus_tag="ACHOS5_005168"
     CDS             2597..>3410
                     /locus_tag="ACHOS5_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9779820.1"
                     /translation="MRLASCFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTCVRDPGRRGYTKHMLRLRRNGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEMRDAMQSLILPPPARQALAQAALTYRYGDEHRPVTTADILTPRRREDYGKDLW
                     STYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLES"
BASE COUNT          940 a          756 c          832 g          882 t
ORIGIN      
        1 cgcctgtggt ggtgtttttc ctggctggtg ttattgaaaa tattttcaga tatatattac
       61 ccggacgatg gttaagattg atttatttaa ttttggggag aaaattatga gactgtcaaa
      121 tattttcctg gctgtagttt taactgtgac cgtatctgta gcacacgcta ttccaaatat
      181 gtggggcagt ggcttcacaa tgggcgttac tgagttcagt attgataacg gtaagtgggg
      241 tatcaatatc agttgcacag gtaaccccaa cgaaaacaat gtgctggcgc acacaatcta
      301 tatttacaaa ggagataagg tggtcgcaaa ctcggaagaa aaaaatatta gtttcgttat
      361 tgacggtgaa gaattctggg gtgtggttcc tgacggtacc cgtatgaacg ataacgcctg
      421 ggttagcttt gtaaaagcaa taagcactgc aaccgggttt gaactgtaca taaacgagaa
      481 gaaagttgcg acgtttaacc cgtctgccaa aaacgttaag aaagtgatgg ataataactt
      541 tatccagtcg tgctggaata cctggtacga ataacgatta caggagaggg ggacttcctg
      601 gcccccgatt tatttaagga gtgcatgaaa tgaaacattt tttaattctg gttatcggtt
      661 tactcatttc catcaccgcg actgccgaat cgctgggtca gtttgaagag gatatcaccg
      721 gttttccgca gggagtatgg ttttacaaag aaaaagcagg ctggaaaaac gaaaccgggt
      781 ataaaaaaga atatttaaac agtctcctct ataaggccga agaaattgcg aattatccgg
      841 gtaacagata cacgatttac cgtagtaaaa acagcagcat aattttcgtc gctgacaacg
      901 aaaccaggac tttctacttg ctaaacaaca aggacgcctt atggtctgat attcagatcg
      961 ggtattgggc tgagaacaaa aactcgtgtc actacgtctt tgtcgatact caataccttg
     1021 cgcaaagtaa cgaacttgac gagttctttc gaaccggggt gctcgatact catggtgttg
     1081 gtgaatcgga tcgtaaagta aaagagatca gacaaaaaca taagactcat gtcttactcc
     1141 gcctgcctgc gcaatgtgtg aatgatgcga tcctcagcag aatgaataag tccgaacgtc
     1201 gtgatctaat gaaagcgcaa gaagatttca aacaatatct tgccagaatg gagctgtaag
     1261 tattcaacct tttcgcaact gtgagtgaaa tgaaaatcaa aactatactt tatactatca
     1321 ttcttttctg tgaaattggc atcaccgaca cttatgcact cggtggttcg gatatccgtc
     1381 actgcgaacc aactgaagaa tctgtaatta ttaacccgga cgagtcaccg gtgttaatat
     1441 atagcttcag taaacacgaa gtgtataaac tgatgcaggg ggatacctgc actgggctag
     1501 ttattagcga taaaaagcca aagcctgtcc acgtttaatg gacatcgctt gcgaaccgcc
     1561 agaagtcatg tctgccagca ttcatctact ggccgcggat aattatacga ccatggattt
     1621 ctgccataac gccgaattaa tcggtcgtat tccgtaagcc gtggtatcga ataatttaag
     1681 aaggattttt atcatgaaac accgacacat tttaacgttc tttgttgctt tcctgttcat
     1741 gactatcgta cctgatagcg acgccgcatt agtaaagcgt cctgagaaag gtcgtgcacc
     1801 gttcccggaa tatgtggagt tcgcgcaaaa ccgtaaccag ttcccatgta atgcacgttt
     1861 acgctttaaa ggtctctgct ttgttgaaca aactgacccc tggggtgcag caagcgttgt
     1921 ccgaatccct ttcaattccc ccaatggtaa aggcagactt aacgcttatg gcgtggttgt
     1981 tcatgcaata atggttggtg atgttgtcgt tggtggcagc tattcaactg gcaagaatgt
     2041 tacctggaat ttcgatacgt tttataggga gggtaaacgt cgcggtaagg tgacctttta
     2101 tgttgatggt aaatactggc actatcgcga ctatccactc gaataatcca taacgaacaa
     2161 aactattaaa ggaattatgc gtcttatctg cgcattatta aaatcaatag taaattcgct
     2221 cctgccatta tggctggggc tttttatttt caggtgaaaa cacatgaggg gctgtacatt
     2281 actcttattt tccgaagccc ctttcttacg cgaacaggag gttgctgtca taacagctta
     2341 ccgcaatgtg tttatccagg atgacccggg aatgcatttc cgttgggtta tccgcaacgc
     2401 ggaaggtcag cgacgctggc gctgccggaa ttctgaaccc gatgccggaa aggtgcttaa
     2461 tacgcggctc gccagtgacg gtcctctaag acaataaacg tcattcattt cacccatcag
     2521 gctgcgtctt ctccgggaga cgtggctttt tcatttatta caccagtaaa tcttaaccac
     2581 cgataaggag caaagtatgc gattagccag ttgttttggt tatgctgcga accagatacg
     2641 ccgtgaccgt ccactgacac atgaagagct gatgcaccat gtgccgggga ttttcgggga
     2701 agagaaacac acgtcccgaa gccagaacta tacgtacatc cccaccatca ccgtactgga
     2761 aagcctgcag cgggaaggct ttcagccatt cttcgcctgc cagacctgtg tgcgcgaccc
     2821 gggccgccgg ggatacacaa aacacatgct gcgcctgcgc cgtaacggag agataaacgg
     2881 agaacatgtc cctgaaatta ttctgctcaa ctctcatgac ggtacctcca gctaccagat
     2941 gctgccgggt tacttcaggt tcgtctgcca gaacggatgc gtctgtggtc agtctctggg
     3001 ggaagtgcgt gttccgcacc ggggaaatgt ggtggacaga gtcattgaag gggcttacga
     3061 ggtggtgggc gtgtttgacc ggatagagga gatgcgtgat gccatgcagt cgctgattct
     3121 gccgccaccg gcacgccagg cgctggcaca ggcggcactg acttaccgtt atggtgacga
     3181 acatcggccc gtcaccaccg ccgacattct gacgccacga cgccgggagg attacggtaa
     3241 ggacctgtgg agtacttatc agaccatcca ggagaatatg ctgaaaggcg ggatttccgg
     3301 tcgcagtgca aaaggaaaac gtatccacac ccgtgccatt cacagcatcg acaccgatat
     3361 taagctcaac cgcgcattgt gggtaatggc agaaacgctg ctggagagcc
//
LOCUS       DAZKHK010000272         3379 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKHK010000272 DAZKHK010000000
VERSION     DAZKHK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.274"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..1687
                     /locus_tag="ACHOS5_005169"
     CDS             137..1687
                     /locus_tag="ACHOS5_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9779821.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTNI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSAKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAVAGFQNSGGMRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            1728..3347
                     /locus_tag="ACHOS5_005170"
     CDS             1728..3347
                     /locus_tag="ACHOS5_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9779822.1"
                     /translation="MKTAQRTLLAATLLTLFSTSVMAQDVTIIYTNDLHAHVDSYKVP
                     YVADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESEKPFWNKPYTILEKDGI
                     KIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTLQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSNVVIDYFKKGHKITPAQVNEMRVEVSK"
BASE COUNT         1063 a          699 c          713 g          904 t
ORIGIN      
        1 aatgtttaat taataacatt atttgttact aagatcacac aatacttttc tgaactatgc
       61 ttacataaaa taagacacga tgtgtctttt atttaaataa ttcaacaaat gtaacaaccc
      121 ctatccagga gggatgatga aaactgtatt tacaacatta tcagtcatat tcggcgtgat
      181 attttctcac agtgttctag ctcaggacgt tactatttat tatacgaatg acattcacgc
      241 acatgtcaat ccggcaaaaa tcatcgctgt tgataagaac agacttatag gtggtatggc
      301 taatattgca ggcatcgtta atgaagcgaa gaaaaaaagc aaagatgtgt ttttctttga
      361 tgcaggcgac tattttaccg ggccgtatat cagcaccctg accaaaggtg aagctattat
      421 tgatatcatg aacactatgc cctttgatgc ggtgtctgtt ggtaatcacg agttcgatca
      481 cggtgtgccc aatatggtgt ctcagttatc aaaagcaaaa ttccccattc tgctgggaaa
      541 tatctactac accgatacga ataaaccagt ttgggatcat ccgtggacca ttatcgaaaa
      601 agatggttta aagattggtg tgattggatt gcatggcgca ttcgctttct atgatacagt
      661 cgcggcaaaa gcacgtgaag gcgtagaagc cagagacgaa attaaatatt taaataaagc
      721 acttgcagaa ttaaaaggta aagtcgacat taccgttttg ctaattcacg aaggtgtccc
      781 ggcgcgtcaa tcaagctttg gtagtaaaga tgtggagcgg ctactacagg cagatattga
      841 gacagctaaa aaagtgaatg gtgttgatgt cttaattacc ggtcacgcac atgtcggaac
      901 accgcaacca ataaaagtta ataacacatt aattgtatct accgatgcat acggcaccaa
      961 catcggaaaa cttgtgcttg attacaatcc gaaaacgaag aaaattgata gttataatgg
     1021 tgagttaatc accatctttg cagatcaatt taaacctgac accatcgttc aaaatactat
     1081 tgataaatgg agtgcaaagc ttaacaaaat aacacaggaa gttgtcggcc attctcccgt
     1141 agttttaaca cgtgagtatg gtagttcctc ttctaccggc aaccttattc ttgatgcaat
     1201 gatggaaaaa acacctgatg ccgttgccgg atttcaaaat agcggtggga tgcgagctga
     1261 ttttcctaaa ggcgatatca cactgggaga tgttattagc acattcccct ttaataatga
     1321 cctcatcgag atggatttga cgggccgcga tctcaaatca ttgatgacgc atgcaaccaa
     1381 tctaactaac ggtgtgttac aggtttcaaa aagcgttgcg gttgtctatg acagcaaaaa
     1441 accactcaac caacggttaa tctctttcac cattaacggc aaacccgtgg aagataatca
     1501 aacatatcgt attgccacgc actccttttg tgccagtggt ggtgacggtt ttgaagcatt
     1561 tttgaatgga aaaaatgtga agacgatacc gggaacaacc tcggcggaat ctatcatcga
     1621 ttatttcaaa aatcataaac ctgtcacccc agacttaact aaacgagtca tggacgtcgc
     1681 caaataaaat ctcatttatc agtcaattta tttaacaagg aatgcgaatg aaaacagcgc
     1741 agcgtacttt acttgccgca acattgctga ctcttttttc gacctcagtc atggcgcagg
     1801 atgtcacgat tatttatacc aatgatcttc acgctcatgt ggattcctat aaagtaccgt
     1861 atgtcgcaga cggaaaacgc gacattggtg gctttgccaa tatatccacg ttagtaaaac
     1921 aagagaaagc caaaaataaa gccacctttt attttgatgc tggagactat ttcaccgggc
     1981 catatatcag tagcctgaca aagggcgaag caataattga tattatgaat accatgcctt
     2041 ttgacgcagt atcaatcggt aatcatgagt ttgaccatgg ctgggataat gccctccgac
     2101 aattaagcaa agcgaatttc cctgttttat taggcaatgt ttatcataaa gagagtgaga
     2161 aacccttctg gaacaagccc tacaccatcc tagaaaaaga tggcatcaag attggtattg
     2221 ttgggctaca cggcgtattt gcatttaatg acacggtctc ggagctctct ctccagggac
     2281 tcgataatga caataataac cgatttgata aatcttccgc gaccctcaag aatcagggga
     2341 ttgaagcacg tgatgaagtg aagtatttgc agcattatat cgacgagtta cgtgataagg
     2401 ttgacctcac cgtggcgctg gttcatgaag gcgtaccggc gcgtcagtcc agcattggca
     2461 atacagatgt tagacgtgcg ctggataaag atattcaaac cgcaagcaag gtgaaaggat
     2521 tggatattct gattaccggg catgcccacg tcggaacgcc agaaccaatc aaagtcggca
     2581 acaccttaat cctttcgact gacagtggcg gtatcgatat tggtaaatta atccttgatg
     2641 tcaaccctac agctcgtact cataaagtga agagctttga gctgaaaacg gtttatgccg
     2701 atgaatggat acccgatcca accacacaaa aagttatcaa tggctggaat aaaaaactgg
     2761 cggatcttgt acgccaacca gtcggtgaat catcgattgc attaactcgt gcctacggtg
     2821 agtcatcgca acttggcaac ctgtttaccg atgcaatgct tgtcgctgca ccgactgcac
     2881 aaattgcgtt gataaactcc ggcagtttac gtgctgatat aaatgcaggc acgattacct
     2941 ttggtgacat taccagtaca ttccccttca agaatgaact tactgaaatg gatctcagcg
     3001 gtaaggatct gcgtaaccta ctggaacacg gggcatcgct taccaatggc atcctgcaaa
     3061 tgtcaaaagg cgcagaaatg cgctataccc tgcagaaacc agtaggtcag cggattgtat
     3121 cttttaaaat taatggtgaa gagatcgttg atacgaatat ttaccatgtt gcgactacga
     3181 ctttccttgc acttgggggc gatggttttc tggcattcaa agaagggaaa aatgtccagg
     3241 tccgcgccgg aaataacatg tccaatgtcg taatcgatta ttttaagaaa ggccacaaga
     3301 ttactcctgc acaggtaaat gaaatgcggg tagaagtaag caaataacaa aaacttataa
     3361 taccattcat tcactggat
//
LOCUS       DAZKHK010000273         3376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKHK010000273 DAZKHK010000000
VERSION     DAZKHK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.275"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..629)
                     /locus_tag="ACHOS5_005171"
     CDS             complement(<1..629)
                     /locus_tag="ACHOS5_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9779823.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCIWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCL"
     gene            complement(629..958)
                     /locus_tag="ACHOS5_005172"
     CDS             complement(629..958)
                     /locus_tag="ACHOS5_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779824.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNTDLKTY
                     SVTLSVPRWEAAALESFLAEHGGWKAFLWTLPYGYRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(955..>3376)
                     /locus_tag="ACHOS5_005173"
     CDS             complement(955..>3376)
                     /locus_tag="ACHOS5_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9779825.1"
                     /translation="LSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDF
                     NKTLVLSGNQSGLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQ
                     SVARFSSASGVEVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDE
                     AGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQE
                     MLIKAEAAFKKADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQD
                     KNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTL
                     MAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRMLEKHAGANEKISQ
                     QRRDLWKAENQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHL
                     NALAQQADKFAQQQRAKRAAIEAKNRGLTDRQAARDATEQRLKEQYGDNPLALNNVMS
                     EQKKTWAAEDLLRGSWMAGLKSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLT
                     GSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGATGGGASASGGTAIQATAA
                     KFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTP
                     GSMADSRSQASGKFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDG
                     GLFSGGGR"
BASE COUNT          635 a         1126 c          831 g          784 t
ORIGIN      
        1 ctcaggcatt tgctgcattt atccttcgtg atatcggacg tcggctggtc atattcatcc
       61 gcgacagccg gaccgctata accgcactcg tcaccgcgat aggtccagat gcaggtgttg
      121 gccagcatga tacgtcccgg aaaaacggca ccatccgttt cagtcggcgt ggacagtaca
      181 aaggaggcac tgaccgcgct cagttcgctg cactgctcga tgcgccagcg gctaatcacc
      241 tcctgctccg gatcggcgtc gctgtttccg ttgacgaagt tcaccgcatc cagaaaacgg
      301 gcataaacct tacgcctgac caccgttccg ccgaccagac tctgcagatc ttccgccatc
      361 ccggtgacca tgccatacag gttagaaacc gtcagcgtgg ggcgcgtact ggtgcctttg
      421 ccattcagtt cgaaaccact cccctgaatg ggatacgcct gatactgtcg cccctgccag
      481 gtgaccggct cacctttttc gttctgctca ttacagaaaa aataacgttc tccaccaacc
      541 tctgtcagat cgatttccca gagcaccacg ctggcagact gctccgcacg ggtgcattca
      601 ttcagtgttt cctgtcggat atcctgcatc agttcaccac ctgttcaaac tctgcgctga
      661 actcaacacg cagcatactg acccgcgacg accattttgc gcaggtcacc tttatctgcc
      721 ggtagccata aggcagcgtc cacagaaagg ccttccagcc cccgtgctca gccagaaacg
      781 actccagcgc cgcggcctcc caacggggaa cagaaagcgt cacgctgtac gttttcaggt
      841 cagtgttcag cccggcaggc gctcgctggg aatagccatc accaaagcgc acctttctta
      901 cggaaggggc cgaagccaca tccataccgg gtttcacttt ccagcggaag gttttcatcg
      961 tccacctccg gagaacagac caccatcgcg catctgcccg gtcacaacat ccattgccgc
     1021 cttacgggct acgtcataaa cagccttcag cgcctgtggc cctatctgac cgttcgtgcc
     1081 gtcgttgtta atcaccacat ggttattctg ctcaaacttc ccggacgcct gcgaccggct
     1141 gtccgccatg ctgcccggtg taccgacata accaccggtg gcatagccgc gcatcagccg
     1201 gtagagattc cccacgccaa tccggctggt tgcctccttc gtgaagacaa attcaccgcg
     1261 gtgaacaatc cccgcaggct catatttgcc gccggttccc gtaaatcccc cggtcgcaaa
     1321 atggaatttc gccgcagttg cctgaatggc tgtaccgcct gacgcggatg cgccgccacc
     1381 ggtagcaccg ccaatggcgc tgccgatact cccgacaatc cccaccattg cctgcttaag
     1441 cagaatttct gtcatcatgg acagcacgga gcgggtgaag ctgcgccagt tctgttcact
     1501 gccggtcagc atcgccgcca tattctgtgc aataccatca aaggtctgcg tggcagcact
     1561 tttaacctgc gacatactgt ccgtggcact ctctttccac tcgctccagc cggacttgag
     1621 gccagccatc cagctcccgc gaagcaggtc ttcagctgcc caggtctttt tctgctctga
     1681 catgacgtta tttagcgcca gcggattatc gccatactgc tccttcagac gctgttccgt
     1741 ggcgtcccgc gctgcctgcc ggtcagtaag cccccggttt ttcgcctcaa tggctgcccg
     1801 ttttgcccgt tgttgctgtg cgaacttatc cgcctgctgc gccagcgcgt tcaggtgctc
     1861 ctgatacgtg accttatcgc caagtacagc cagctggcgt ttgtactcca gcgtctcatc
     1921 tttatgcgcc agcagggatt tctcctgtgc ggatagctgg cgacgttgtg ccgcctcctc
     1981 cagtaccgcg aactgatttt ccgccttcca caaatcccgg cgctgctggc tgattttctc
     2041 attcgctccg gcatgcttct ccagcatccg gagttctgcc tgaagcgtca gcagggcagc
     2101 atgagcactg tcttcctgac gatcgcccgc agacaccttc acgccggact gtttcggctt
     2161 tttcagcgtc gcttcataat cctttttcgc cgccgccatc agcgtgttgt aatctgcctg
     2221 caggattttc ccgtctttca gtgccttgtt cagttcttcc tgacgggcgg tatatttctc
     2281 cagcggtgtc tgcagccgtt cgtaagcctt ctgcgcctct tcggtatatt tcagccttga
     2341 tgcttcggta tcgctctgct gctgcgcatt tttgtcctgt tgactctgct gttcagcctt
     2401 ctttcgggcg gcttcaagcg caagacgggc cttttcacga tcatcccagt aacgcgcccg
     2461 cgcttcatcg ttaacaaaat aatcatcctt gcgcagattc cagatgtcgt ccgctttctt
     2521 aaacgcagcc tctgccttaa tcagcatctc ctgagcggta tcaggacgac caatatccag
     2581 caccgcatcc cacatggatt tgaatgcccg tgctgtcctg tctgcccagg tttccagcgt
     2641 gcccatgttc tctttcaggc gtcgggtctg gtcatcaaac cctttcgttg ctgcctcgtt
     2701 cgccgcctgc aatgccccgg cttcatcgcc ggaacgctgc aactgagcaa catacgcaat
     2761 ctgctccgcc gtcacgttat ggaactggcg cgccatcgcc gtcagccccg acgtcgggtc
     2821 agtggtcaac ttcccgaagg cttcagcgac cttgtccacc tccacgccgg atgcagaaga
     2881 gaaacgcgcc acactctggc tgatggacgc aatctgagcc tcaccgctta cccccgcctt
     2941 aaccagtgca ctgagtgact cgctggtctg gttaaacgtc agccctgccg cctgcccggc
     3001 tctggacagg accagcatac gatctgccgt cagacccgac tgattaccgg aaaggaccag
     3061 cgttttgttg aaatcggata gggttgagtt accctgatac caggcatacg ccagcgcacc
     3121 ggtcgccacc gccagcgagg tggccccgac catcggcagg gtgatcgcac cggcaagccc
     3181 ccggaacatg gggatcatcc cgccgaagga gtccttaacc tgaccaccct gttgcagcag
     3241 gatcagccac gggctttgcc cgcctgcaag ctgcgtggcc acgtcggtga actgtgcagg
     3301 cagcatacgc atggcggctt tatactgccc gacggaaatc cccgctttct gtgcagccag
     3361 cgcctgtcgg ctcagc
//
LOCUS       DAZKHK010000274         3348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKHK010000274 DAZKHK010000000
VERSION     DAZKHK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.276"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..389
                     /locus_tag="ACHOS5_005174"
     CDS             <1..389
                     /locus_tag="ACHOS5_005174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779826.1"
                     /translation="SVVAYFAKSANATEVFLYFIGFTTENKKFKHPEFFIHQLQQMDT
                     RKESFFQIENLPPGAISALNSAISLYAIGMRNNADNLVKLGIYLLISGLFEGYHVDYV
                     CDEISKQAIAEHQREKASKPRKSLLQ"
     gene            725..934
                     /locus_tag="ACHOS5_005175"
     CDS             725..934
                     /locus_tag="ACHOS5_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779827.1"
                     /translation="MNLKKSDLSTDRLTRAEAAAYLGVNNQTLANWACTGKVKIPFHK
                     LGRKVLYMRTDLDAYLASTRRTQTV"
     gene            complement(1282..1461)
                     /locus_tag="ACHOS5_005176"
     CDS             complement(1282..1461)
                     /locus_tag="ACHOS5_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9779828.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKEQE"
     gene            1661..1864
                     /locus_tag="ACHOS5_005177"
     CDS             1661..1864
                     /locus_tag="ACHOS5_005177"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9779829.1"
                     /translation="MRNHTTHPQGRDSHDLNKYIWRFIVLSTAQPRVIHIVATSEQEA
                     RQQSPAGCVMVFAARIRQGVCHA"
     gene            1857..2177
                     /locus_tag="ACHOS5_005178"
     CDS             1857..2177
                     /locus_tag="ACHOS5_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779830.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGHFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            2184..2483
                     /locus_tag="ACHOS5_005179"
     CDS             2184..2483
                     /locus_tag="ACHOS5_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9779831.1"
                     /translation="MRTYLSGLTASGYAHPQIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSRVNVDKKLQELKNMIAAWREQK"
     gene            2480..>3348
                     /locus_tag="ACHOS5_005180"
     CDS             2480..>3348
                     /locus_tag="ACHOS5_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9779832.1"
                     /translation="MKKAPNLKHQSRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFRGHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKW"
BASE COUNT          981 a          698 c          795 g          874 t
ORIGIN      
        1 caagtgtagt tgcgtatttt gcaaaatcag cgaatgctac agaggttttt ctttatttta
       61 ttggctttac gacagaaaat aaaaagttta aacatccaga attttttatc catcaattac
      121 agcaaatgga tactagaaaa gaatcttttt ttcagattga aaacttgcca ccaggcgcaa
      181 ttagtgcgtt aaattcggca atctcactgt atgcaatagg aatgagaaat aatgctgata
      241 atcttgtaaa acttggaata tacttgttaa tttcagggct ttttgaaggt tatcatgtgg
      301 attatgtttg tgatgaaata tcaaagcaag ctattgctga acatcaaaga gaaaaagcaa
      361 gcaaaccaag aaaatcatta ttacaatgaa gttatgcacg taattaaatt aacgtgggaa
      421 aagtacccta aagccagtaa aacaggttta ttgaacgagc tttctgttta ctatcatggg
      481 aaagtaagta aaaacactct ggataaatgg attaaagaat caggagcaca accaccaaga
      541 cctgataaat attcttcgtt tgaacttgtt cacccccaat agctggctag tgtatcttaa
      601 tagctggcta ttaaggctaa ataactggta acctccccac agcaactggc tattgggcta
      661 aaaacaaaat acccattgat tgatatttat caccgttgtt acttagaagt acacggtgat
      721 agatatgaat ttaaagaaat ctgatttatc aactgaccgc cttacacgtg ctgaagctgc
      781 tgcctacctt ggcgttaaca atcaaacgct ggcgaactgg gcctgtactg gcaaggtaaa
      841 aatccctttc cataagctgg ggcgcaaagt gctgtatatg cgcactgatc tcgatgctta
      901 tcttgcatcc actcgcagaa cgcagacggt gtaaggggga gtgatggcac ataaaacaaa
      961 ggcgaccgca gagggccgcc agtggcataa cactaaactt gagcatatcc agaataccag
     1021 gttgttggct ggtgggcaat gctatcagtc tggctcgatt cgctgccatg cctgcaatga
     1081 gcgtattttc ctagaatact ctttaaggta cttctcaagg ataaacgcac atggtgcgaa
     1141 tctctccggt tcatgttcgt ttaccgcatt tttgcgttgt tttttcttgg atgaggataa
     1201 tttctttaat gatttgtcag tcatcgtaga tacctgtaac cctgtgcgcc acagttcacc
     1261 gcaccacggc gctggtgatg gttactcctg ctctttggcc ttgcgacgct ggcggtattc
     1321 aacttctcgc tttaatgctg ctgttacaaa ctgccctgta ctttcaccag gcatttttac
     1381 cgcctcaaca ttgttcataa cttcatgcgg aacccttgcc gcaactgttt gtgattttgc
     1441 gtttactgct tttgtcgcca tgttgtgtac cccttacaaa aaacaaatgc agtatgcagg
     1501 aaaaaaaata agtgttcaac acttgacgtg tttaacacct aggattaaat tggtgttcaa
     1561 caccttgttg atgcaaggtg cagaaacgac gaaacccggc agtgcgctaa cactaaccgg
     1621 gcttctaacc accaacgata gaaagcgtat cgaggtagct atgagaaatc ataccacaca
     1681 cccgcaaggg cgggactcgc acgacctgaa taaatacatc tggcgtttta tcgtcctgag
     1741 caccgcacaa ccgcgcgtga ttcacatcgt ggccaccagc gaacaggaag cacgccagca
     1801 atccccggct ggctgcgtga tggtattcgc cgcccgtatt cgtcaggggg tgtgccatgc
     1861 ctgatatgtc aaattaccag tacctgatta atccgcattt taactgtgag catgatattg
     1921 ctaaaaaggt ttattccgct gcggatgggg ctactgacaa tatatcaatg gctgttgcgt
     1981 caattggtag cctgatgtgg catgcgtcag aaaatgagga atatgacgaa aaggccatgc
     2041 gcattgatat gggtaatatc ggtttgttac tggcaatgct tggacatttt gatatttcgt
     2101 tacggtgcac cattgaaaat gccacagatg cattaaatgc tataaagaaa gcgaatactg
     2161 attcaaatcg gggataaata atcatgagaa cgtatttatc tggcttgact gccagcggtt
     2221 atgcacaccc ccaaattatc cccggcgcta tttatctgga taagaacggt aacagagtaa
     2281 cggtaaaaga actgatgttt gaccgtgtgt attttattcg tgatggctat tcatttcata
     2341 gttcgctgaa cgtggagatc tttattagca gattcaggcg ggaaatcccg ctttccagaa
     2401 ataaccatgt gtcacgtgtg aatgtggata aaaaactaca ggaactgaaa aacatgattg
     2461 ccgcgtggag agagcagaaa tgaaaaaagc gccaaattta aaacaccagt cgcgtgacaa
     2521 aatgacggaa gtcatcattt ttgcgggtag tgatgcgtgg gcacatgcga agcagtggca
     2581 ggaacaggac gggcgactgg ctggcgataa cgtgcctcct gtctggcttg gagagcaaca
     2641 acttgccgaa ctggacaacc tgcaaatcgt accggacgga cgctatcgcg tgcgtctcta
     2701 tcaggcgggg ttattgcgtc cggggcttgt taataccatc gggcagaaac tggcagcggc
     2761 aggtgtcagg gatgctgatt attatcctga aggaatgcac agccagaaac gggagaactg
     2821 gcgcgaatat ctggaacgtg aacgggcaga gcaggcggaa aagaaaaagg tagttgaact
     2881 gcctgtaaag aaaaaagagc gggtaaaaga cgataacgct tcatcactgg cgcttaacca
     2941 gatgggagca agtcaacgcg gcgaagttct cctggcacat tatggcggtg aattggcgat
     3001 tcatgctgac tctgacactg ttcaccatta caacggcgtt gtatgggagc cggttcagga
     3061 taaagaatta cagcgagcta tggcacagat tttcattgat gcggagatca gctattcgca
     3121 gaacgccatt aaatcggcgg tcgataccat gaagttaagt ttgcctgtaa tggggaatac
     3181 agcccgtaac ctgattggat tcagtaacgg ggtatttgat accagaacag gtaattttcg
     3241 ggggcataac aaaaacgact ggttgttaat tgccagtgaa ttacctttca gcccaccagc
     3301 agagggggaa acactggcaa cacatgcgcc gaatttctgg aagtggtt
//
LOCUS       DAZKHK010000275         3345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKHK010000275 DAZKHK010000000
VERSION     DAZKHK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.277"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(46..996)
                     /gene="dusC"
                     /locus_tag="ACHOS5_005181"
     CDS             complement(46..996)
                     /gene="dusC"
                     /locus_tag="ACHOS5_005181"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP9779833.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            1235..1633
                     /locus_tag="ACHOS5_005182"
     CDS             1235..1633
                     /locus_tag="ACHOS5_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9779834.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1630..2325
                     /locus_tag="ACHOS5_005183"
     CDS             1630..2325
                     /locus_tag="ACHOS5_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP9779835.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2455..3339
                     /gene="cdd"
                     /locus_tag="ACHOS5_005184"
     CDS             2455..3339
                     /gene="cdd"
                     /locus_tag="ACHOS5_005184"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9779836.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
BASE COUNT          735 a          885 c          856 g          869 t
ORIGIN      
        1 gctgaataag cgattcattg tctcagataa gctgcgattc cactattatc gcagcttatc
       61 gatatcaatg gcctgaatag cccttgcaat atcaggggaa ttattcaaca cccgaacatg
      121 ctgaaataat tccgttgctt catcgtattc tttacgcaaa taactcaacc actgtttaat
      181 ccgcgcaacg tgatataacc cggtatcgcc ctgcttttcc agacgggtat atttttgcag
      241 caaagcaacc acctccggcc acggcattcg cggttcgtta tatttcacca cacggctcag
      301 gttgggaata ttgagcgccc cgcgaccaat catcactgcg tcgcagccgc tgatcgccat
      361 gcattgttgc gcactctgcc agtcccagat ttcaccgttg gcaatcaccg gaatattaag
      421 ccgctggcga atctcgccaa tcgcctgcca gtcaatatgt tccgcgcggt aaccctgctc
      481 ttttgtccgc ccatgtacta ccagttccgt agcaccagcc tgttgaaccg catcggcgat
      541 ttcaaatttc ttctcaccgc tgtcccagcc cagacgcact tttacgctga caggcaaatg
      601 cgccggtaca gcttcgcgca tcgcttttgc accctggtag atgagttcag gatctttcag
      661 taacgttgcc ccaccaccgc taccgttaac cgttttcgac gggcagccgc agttaagatc
      721 cacgccccag gaacctaact ccaccgcacg ggcggcgttc tctgccagcc actgtgggaa
      781 ctgccctaac aactgcacgc gcaccagcgt accggatggt gtccggctgg cattttgtag
      841 ctcagggcaa atgcgatgaa agacttttac tggcagcagt tgatccacca cgcggacaaa
      901 ctcggtgatg cacagatcgt agtcgttaac ttcggtcagt aattcccgca ccagagagtc
      961 aagtacaccc tccatcggag ccagtaacac acgcatatca tcacccgcaa aaaaatgagg
     1021 cgctatgtta gcgcctccgg tcaacggatt aaaggtctgc aaagagggat tgctgcaaac
     1081 tgcaatccct ctctatgctt gatgtatgcc tgagttcgct gcgcattgcg taataccggg
     1141 gagcgaaaaa ataagcgctg atttcttaat gtgatcggta gcacgtttta cgaattaatt
     1201 gtatgatgaa tccatctcat ctggggtgtt gattatgagt aagacactga acattatctg
     1261 gcaatattta cgcgctttcg tcctgattta tgcctgcctg tatgcaggca ttttcattgc
     1321 ttccctgcta ccggtaacca ttccgggcag catcatcggg atgctgatcc tgtttgtcct
     1381 gctggccttg caaattcttc cggcgaaatg ggtcaatccg gggtgctacg tactgattcg
     1441 ctatatggcg ctattgtttg tgccgattgg cgtaggcgtc atgcaatatt ttgatttgct
     1501 ccgcgcacag tttggtccgg tagtggtttc ctgtgcagtc agtacgctgg tggtttttct
     1561 ggtggtgagc tggagttcac aactggtgca cggtgaacgt aaagtcgtag gtcagaaagg
     1621 atcagaagaa tgatggcgaa tatctggtgg tcattaccgc tgacgttgat tgtctttttt
     1681 gccgcccgca aactggcggc acggtataag tttcccctgc ttaacccgtt gctggtagca
     1741 atggtggtga tcattccttt tttaatgctg actggcatct cttacgacag ctactttaaa
     1801 ggtagcgaag tgctaaacga tctgctgcaa ccggcggtcg tcgcactggc ttatccttta
     1861 tatgagcagc tacaccagat ccgcgcgcgc tggaaatcga tcatcaccat ctgttttatc
     1921 ggcagcgtgg ttgcgatggt gacggggact tccgtggcat tgttgatggg cgcttcaccg
     1981 gaaatcgccg cgtcaatcct gccgaaatca gttaccacgc ctattgcaat ggcggttggc
     2041 ggcagtattg gcggtattcc ggcaatcagc gcagtttgcg tgattttcgt cggcatcctc
     2101 ggcgctgtgt ttggccatac attgcttaat gcgatgcgta ttcgtaccaa agctgcgcgc
     2161 ggtctggcaa tgggcacagc ctcgcacgcc ctcggtacgg cgcgctgcgc cgagctggat
     2221 tatcaggaag gtgcatttag ttcgctggcg ctggtgttat gcgggattat tacttcgctg
     2281 atcgcaccgt tccttttccc gattattctg gcagtaatgg gctaaaattt gcgatgcgtc
     2341 gcgcattttt gatgtaagtt tcacgcgttg cataattaat gagattcaga tcacatataa
     2401 agccataacg ggttcgtaaa ctgttatccc attacatgat tatgaggcaa cgccatgcat
     2461 ccacgttttc aaaccgcttt tgcccaactt gcggataact tgcaatctgc actggaacct
     2521 attctggcag acaagtactt ccccgctttg ttgaccgggg agcaagtctc atcgctgaag
     2581 agcgcaacgg ggctggacga agacgcgctg gcattcgcac tacttccgct ggcggcagcc
     2641 tgtgcgcgta cgccattgtc gaattttaat gttggcgcaa ttgcgcgcgg tgtgagcgga
     2701 acctggtatt tcggtgccaa tatggaattt attggtgcga caatgcagca aaccgttcat
     2761 gccgaacaaa gcgcgatcag ccacgcctgg ctgagtggtg aaaaagcgct tgcagccatc
     2821 accgttaact acacgccttg tggtcactgc cgtcagttta tgaatgaact gaacagcggt
     2881 ctggatctgc gtattcatct tccgggacgc gaggcacacg cgctgcgtga ctatctaccg
     2941 gatgcctttg ggccgaaaga tctggagatt aaaacgctgc tgatggacga gcaggatcac
     3001 ggctatgcgc tgactggtga tgcgctttct caggcggcga ttgcggcggc aaaccgttcg
     3061 cacatgcctt acagtaagtc gccaagcggt gtcgcgctgg aatgtaaaga cggtcgtatt
     3121 ttcagcggca gctacgctga aaacgccgca ttcaacccga ctctgccgcc gttgcagggg
     3181 gcgttaattc tgttgaatct caagggttat gattacccgg atatccagcg cgcggttctg
     3241 gcagaaaaag ccgatgcgcc gttgattcag tgggatgcca cctccgcaac gctgaaagct
     3301 ctcggctgtc acaatatcga ccgcgtgctt ctcgcttaag cctag
//
LOCUS       DAZKHK010000276         3304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKHK010000276 DAZKHK010000000
VERSION     DAZKHK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.278"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..633)
                     /locus_tag="ACHOS5_005185"
     CDS             complement(<1..633)
                     /locus_tag="ACHOS5_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFP9779837.1"
                     /translation="MKIALMNEFSQASKNAIIFNELVAASAHQGHTIFNVGMCDDNDH
                     RLTYVHLGIISAILLNSRSVDFVITGCGTGQGALMALNTFPGVTCGYCIEPTDAYLFA
                     QVNNGNALSLPFAKGFGWGAELNMRYIFEKAFDGEKGLGYPAERRESQNANAIILSNM
                     KEAVSKPLMDALQAIDPELLKQALGGEKFQKCFFNNSKDKELVNYVKNLLD"
     gene            902..2074
                     /locus_tag="ACHOS5_005186"
     CDS             902..2074
                     /locus_tag="ACHOS5_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HFP9779838.1"
                     /translation="MAKGDIKKLKFESFYDSESNNEVIRLTPTDILCHRNYFYQKCFT
                     NDGKKLIFAAEFDCNRNYYLMDLVTQTAKQLTEGAGDNTFGGFLSPDDNYLFYVKNEK
                     HLQRVDLTTLEEVTIYTVPDNWVGYGTWVANTDCTEIVGIEISKTDWTPLSDWKIFLE
                     FYHKNPRCRLITIDLETGNAEVILDRNTWLGHPIFRPNNNDTIAFCHEGPHDLVDARM
                     WMINRDGTNERKVHEHQPGESCTHEFWIPDGSALAYVSYMKGQQQRYIRKYNPDTDED
                     SLVMEMPACSHLMSNHNGILYVGDGSGSPVDVKDTCSYKIENDPWIYILSSKNKKIHK
                     LVKHNSSWSVQKGDRQVTHPHPSFSPDNKYVLYSCDAEGEPALYMAKIPEEILTQQ"
     gene            2104..2904
                     /locus_tag="ACHOS5_005187"
     CDS             2104..2904
                     /locus_tag="ACHOS5_005187"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9779839.1"
                     /translation="MDFINMFSLKGKNALVTGASYGIGFEIAKALSNAGATIIFNDVI
                     EENIKKGLAAYKENNINAHGYLFDITDEQSVKENIKKIESDVGIIDILVNNAGIIQRK
                     PMLETTAADYRKIIDIDLTGQFIMAKAVLPSMIKKGHGKIINICSMMSELGRETVVGY
                     ASAKGGLKMLTKNICSEFGEKNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQFII
                     SKTPAGRWGKPDDLAGAAIFLASDASNFINGHILYVDGGILAYIGKQP"
     gene            2959..3282
                     /locus_tag="ACHOS5_005188"
     CDS             2959..3282
                     /locus_tag="ACHOS5_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP9779840.1"
                     /translation="MFVFNEDTAIEDLGNGVKRKVLAHGGNMMAVEVHFSKGAIGSLH
                     HHQHEQLTYVLSGIFEFTIGDEVRTVKTGDTLYKQPNIVHGCKCLEKGVLLDIFTPQR
                     QDFLK"
BASE COUNT         1057 a          587 c          661 g          999 t
ORIGIN      
        1 atctaataaa tttttaacgt aattaaccaa ttctttatct ttactattat taaagaagca
       61 tttctggaat ttttcgccac caagagcttg tttaagtaac tccggatcga tggcctggag
      121 agcatccatg agtggcttac taactgcttc tttcatatta ctcagtataa tggcattcgc
      181 attttgtgat tcacgacgtt ctgccggata gcctaatccc ttttcgccat caaatgcttt
      241 ttcaaaaata tagcgcatgt ttaattctgc cccccagcca aaaccctttg caaatggcaa
      301 ggacagtgca ttcccattat ttacctgtgc aaacaggtag gcatctgttg gttcgatgca
      361 ataaccacaa gtaactcccg ggaatgtgtt cagtgccatc aatgccccct gtcctgtacc
      421 acagccagta ataacgaaat caacagatct gctgtttaac aagatagccg aaataatgcc
      481 gagatgaacg taagtcagac gatgatcatt gtcatcacac attcctacat tgaaaatagt
      541 atggccttga tgtgcgcttg ctgcaactaa ctcattaaaa attattgcgt ttttagatgc
      601 ctggctaaat tcattcatca atgcgatttt catacgaatt cctcatttaa aatcatactg
      661 ttaaacttca tagcataaca acatgaacga tatgataata cattacaaac attacttgca
      721 acattgtatt tgaaaattca aaacatcatt tcatttttat tgttgtgtgc tctcgatcac
      781 ggtttgctgc acttttgaac gctatattgg atgctaacga aacgatagaa acgatagaaa
      841 cgatagaaac gatagaaacg atatgatgaa gtattgtcat tcttataaag gataagatta
      901 tatggctaag ggtgatatta aaaagttgaa gtttgaatct ttctacgata gtgaatcgaa
      961 taacgaagtt attcgattaa cgccaacaga tattctttgc catcgcaatt atttttatca
     1021 aaaatgtttt acaaatgatg gtaaaaaatt gattttcgct gctgagtttg attgtaaccg
     1081 gaattattat cttatggatt tggtaactca gacagcgaaa caactcactg aaggagctgg
     1141 agataacacc tttggtggct tcctgtcccc tgatgataat tatctttttt atgtgaagaa
     1201 tgaaaaacat ttgcagcgtg ttgatttgac cacattagaa gaagttacca tatacacagt
     1261 accggataat tgggtggggt acggaacatg ggttgctaat actgattgta cggaaatcgt
     1321 cggaatcgaa atcagtaaaa ctgactggac cccgcttagc gactggaaaa tatttctgga
     1381 attttatcat aagaatccac gatgccgttt aattacgatt gatctggaaa ccggaaacgc
     1441 tgaagtgata ttggatcgta atacttggtt agggcatcca atttttcgtc cgaataataa
     1501 cgatactatc gccttttgcc acgaagggcc ccatgacctg gtggatgcaa gaatgtggat
     1561 gattaaccgt gatgggacaa atgagcgtaa agtgcatgag caccaacctg gtgaaagttg
     1621 tacacacgag ttctggatcc ctgatggatc tgcactggct tatgtttcct atatgaaagg
     1681 acagcaacag cgttatattc gcaaatataa tcctgatact gatgaagatt cattagtgat
     1741 ggaaatgccg gcatgttccc atttaatgag taaccataat ggtattctct atgttggtga
     1801 tggttcaggt agtccggttg atgttaaaga tacctgcagt tataaaattg aaaatgatcc
     1861 ctggatttac attcttagca gtaaaaataa aaaaatacat aaattggtga aacataatag
     1921 ttcctggagt gtacagaaag gagaccggca ggtaacgcac ccgcatccat cattttctcc
     1981 agataataag tacgttttat attcttgcga tgccgaaggt gaaccagcac tatatatggc
     2041 taaaattcct gaggaaatct taacccagca ataactgaca aatttttcac tattggagtt
     2101 tttatggatt tcatcaatat gttctctctt aaaggtaaaa atgctttagt cactggagca
     2161 tcatatggta ttggttttga gatcgctaaa gcactaagta acgctggtgc aacgatcatt
     2221 tttaatgatg taatagaaga aaacattaaa aaaggcctgg cagcatataa agagaacaac
     2281 atcaacgcac atggttattt atttgatatt acagatgaac agtctgtgaa ggaaaatatc
     2341 aaaaaaatag agagtgacgt aggcattatt gatattctgg taaataatgc agggattatt
     2401 cagcgtaaac ctatgctaga aactacagca gctgattatc gcaaaataat agatattgat
     2461 ctgacaggtc agtttattat ggctaaggcg gtattgcctt caatgattaa aaaaggacat
     2521 ggcaagatca ttaatatctg ttccatgatg agcgaattgg gtcgcgaaac cgttgtaggc
     2581 tacgcttcgg caaaaggcgg cttaaaaatg ctgactaaaa atatctgttc tgagtttggt
     2641 gaaaaaaata ttcagtgtaa tggtattggt ccaggctaca ttgctacacc tcaaacagca
     2701 ccgttacgtg aaattcaacc agatggcagc cgccatccat ttgatcagtt tattatatca
     2761 aaaacgccag ctggacgctg gggtaaacca gatgatcttg ctggtgcagc tatattttta
     2821 gcatcggatg cttcaaactt tatcaatggt catattttat atgtcgatgg tggcattctg
     2881 gcctacattg gcaaacagcc ttagttatat tcagtcataa ctgtatgtac tactttaaat
     2941 attttcagag aggtggtgat gtttgtattt aatgaagata cagcaataga agatttaggc
     3001 aacggggtca aaagaaaagt tttggctcat ggtggcaata tgatggcagt agaggtccat
     3061 ttctctaagg gggctatcgg ctctttacat caccaccaac atgaacagtt gacgtatgtc
     3121 ttatcaggta tttttgaatt taccatcggc gatgaagtgc gtacagtaaa aactggtgat
     3181 actttatata agcagcccaa cattgtgcac gggtgcaaat gtctagaaaa aggagttttg
     3241 cttgatatat ttacacctca acgtcaggat tttttaaagt aaatacccag tattaatatg
     3301 aact
//
LOCUS       DAZKHK010000277         3273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKHK010000277 DAZKHK010000000
VERSION     DAZKHK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.279"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..210)
                     /locus_tag="ACHOS5_005189"
     CDS             complement(<1..210)
                     /locus_tag="ACHOS5_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP9779841.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMG"
     gene            331..609
                     /locus_tag="ACHOS5_005190"
     CDS             331..609
                     /locus_tag="ACHOS5_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779842.1"
                     /translation="MKKCVTVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLASLERQKKSLR"
     gene            complement(601..891)
                     /locus_tag="ACHOS5_005191"
     CDS             complement(601..891)
                     /locus_tag="ACHOS5_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779843.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            1132..2628
                     /locus_tag="ACHOS5_005192"
                     /pseudo
     CDS             1132..2628
                     /locus_tag="ACHOS5_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
     gene            complement(2651..3184)
                     /gene="cui"
                     /locus_tag="ACHOS5_005193"
     CDS             complement(2651..3184)
                     /gene="cui"
                     /locus_tag="ACHOS5_005193"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="HFP9779844.1"
                     /translation="MINKHHQTDKGNKIATKMFYFLIISLIPMFSIIIIYIHNPTSNI
                     LNYIALRANDLPAIYSQNTPVFSKTLSLYVKTAPFTAILLFLNTCKNLRLKNDKSPWQ
                     LLKINISFTFFYAILIYAFLFTDTELTSSAKMLKLMSVNIILLSFFYISLHSIIFIFS
                     YLYFWFCIGSYRSFKEK"
BASE COUNT          980 a          510 c          792 g          991 t
ORIGIN      
        1 ccccatatcc cggaactgct gaaagaacgg gagcatcatg cgtatggtgt ctgctttctg
       61 ggtgtaaaag ttaggatcaa atccatcatc tgaagcataa acataatgcc gggtatgtcg
      121 ttccaggctg gtctttggtg tgcatcctgt aagagctatt gttgctgtta tcagaggtat
      181 cagaagcagc ctggcagcgg cagatctcat aaacatctcc ttgcgttttt aatcttgata
      241 ctcagggagt tatatttccg aagagtatct cttatcatac gtcggtttac cccaatgtga
      301 tttgatggtc aatgagtcag gaataatccc gtgaaaaaat gtgtgacagt cctgcttttt
      361 agtgttcttt ctgcttcggt tgccagtcat gcggacagca atgtttttca acaaaaggcc
      421 gagaagacaa aaaacggcat tgttgcggat gttaatgcca gacagaaaat gttcagtaac
      481 agactgaaga tacaacttca gatcgatgag ttgagagcag agctggctgg caccagagat
      541 gcccaaaaaa gagaggattt gcgtaaaaaa ctggcgtctc tggagcggca aaagaaatcc
      601 ttacgctgat gggggcgtat tttccccggt ttcttccata acctttttac cgttcagata
      661 tttacgggcg aggcgaatgc gtcttgcctg gtataccgcc gagtaagcct gggtgggatt
      721 cacaccatta ctgatttcac tgaccacatg acgctgatct gccagtgtca gcgtgtaaac
      781 ctgtttctgg atgaggtcac gggctttgtc atgctcatat ccggcagcaa caagggtgtc
      841 gagaagtcgt ttgtctgttt tgttcagttt atggcggggt gatttgctca catcgttctc
      901 cgttttttca tgatccaaca gttttatatc tcatcctgat ttgcaccggt gttctgtttc
      961 gacgttctca atgcctgcct ccttgtagtg tgatctttat tgcaaaaatg aaaatccatg
     1021 ttcttgacat gggcaatgcc gagtagtagg ttttcgctgt acataaaacc agtggttata
     1081 tgtacagtgc attgttttta atttattgtt ttaattatca aagaggaatt tatgactaaa
     1141 gaactaagtg gatatggccc aactgctggt gagtctgtgg ggggaacagg ggcgaatctg
     1201 aatcagcaag gtggaaataa taatagtggg gttcactggg gtggtggttc aggctctggt
     1261 aatggaggtg gtgaacatgg aagtcagacg gggtggggat ggagtaaaac caataatccg
     1321 gatgtgccgc cttatgttga tgataatggg caggtgcgca ttaccattac aaatggtctg
     1381 gtaaaaacgc ctgtatatgg agttcctggg gctggcggaa atagtgatgt tcaggggggg
     1441 ctatattcct gaaaatccta atgatgaagt tgctcgtaag tgggataaaa acaacttacc
     1501 gcgagagatt gatgtgagta tcgatggttt taaatatcgt gtaacgctta atgataatgg
     1561 tagagctatc ggtattcttc gaacaggtgt ccgtccttac gtagggagtg aaaaagctaa
     1621 ggcgggtatt atggaaaaaa tcaatcataa aacacctgag gagatatatg aggtgttagg
     1681 ttttaataaa gatgaatctc agcggcagga aaaagcaaaa caacaggctg aagatgcgtg
     1741 ggatcgtttg cctcctaatg tgagaaaatt tgatgttgat gttgatgttg aacagttcca
     1801 ctatttagtg gtgcttgatg attatggtaa tgttttatct gtaactagaa cagctgttcg
     1861 tccttatgta gggagtgaaa aagctaaggc tggtattatg gataaagtcg accataagac
     1921 cccagaagag atatacgagg cattaggatt taataatgaa gagcctcaac gacagaatca
     1981 agctaaaaaa gctgcttatg atgtgtttta ttctttcagt atgaaccgtg accggattca
     2041 gagtgatgtg ctaaataaaa cagctgaact tatatctgat atgggtaaca agatcggtga
     2101 ttatcttggt gaatcttata agtcgttagc aagagaaatt gctgatgata taaaaatttc
     2161 caggggaaaa ccatccgtag ctacgatgat gcaatggagt cattaaataa aattttgtct
     2221 aatcctagtt ttaaagttaa cagagcagac cgtgatgcac tggtgaacgc ctggcgaagt
     2281 cgtgatgctc aggatatggc aaataaactg gggaatatca gtaaggcttt caaagccgcc
     2341 gatgtgatta tgaagattga gaaggtcaag gagaaaagca tcgagggata tgaaactgga
     2401 aactgggggc cgctgatgtt ggaggttgag tcttgggttc tcagtggtat gaccgcaagc
     2461 gttgccatga cattgcttag tgctataatt ggatcattca cactaccggc gtttgcatta
     2521 actgcaataa atattgcagg tattatggga attagttacc ttgcatcata tattgatgca
     2581 agtgtggctg aaaaaataaa taataaattg gttcatttta ctcattgata gaaaaagaga
     2641 ggtgatcata tcacttctct ttaaacgacc tataggaacc aatacaaaac caaaaataca
     2701 aatatgagaa tataaaaata atggaatgta agctaatgta aaaaaacgat aggaggatta
     2761 tatttacaga catcagtttt agcatttttg cagaactggt tagctccgta tcagtaaata
     2821 aaaaagcata tattaagata gcataaaaaa aagtgaaaga aatatttatt ttcagtagtt
     2881 gccatggaga tttatcattt tttaatctaa gatttttaca tgtgtttaaa aataaaagaa
     2941 tggcagtgaa aggagctgtt tttacataca gactcaatgt tttgctaaat acgggggtgt
     3001 tttgtgagta tatggcaggt aggtcatttg ctctcaatgc tatatagttt aatatattgg
     3061 atgttggatt gtggatatat ataatgatta ttgagaacat tggaattagt gaaattataa
     3121 ggaagtaaaa catctttgtt gctattttat ttcctttgtc tgtttggtgg tgtttattta
     3181 tcatctgttt acctgtgaat ttattagatt aaagattttg atgaatggct atgggttatt
     3241 ttctctttag attgtaatat atgaaacttg gca
//
LOCUS       DAZKHK010000278         3156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKHK010000278 DAZKHK010000000
VERSION     DAZKHK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.280"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..1023
                     /locus_tag="ACHOS5_005194"
     CDS             130..1023
                     /locus_tag="ACHOS5_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9779845.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIESGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1054..2043)
                     /locus_tag="ACHOS5_005195"
     CDS             complement(1054..2043)
                     /locus_tag="ACHOS5_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9779846.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGNDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2070..2921)
                     /locus_tag="ACHOS5_005196"
     CDS             complement(2070..2921)
                     /locus_tag="ACHOS5_005196"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9779847.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLSAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT          766 a          755 c          801 g          834 t
ORIGIN      
        1 ctgaaagttt agaattattt tcgatacagc cagattatca ttgttactaa taatgtggtt
       61 aaaataatag catctatgcc ccccttcatg agtgtctgtg aagaggatgc ttctacaagg
      121 aaatcgccaa tgctcaaaga aaacttcaac gaactgcaaa tctttcttgt ggtggcaaga
      181 gagcgaagtt ttaccaaagc agcgggcaaa ctcggcgtct ctcagtctgc acttagccat
      241 gcaatgaagg ctctggagga aaggctcaat atccgcctcc tgacacggac tacccgcagt
      301 gtggctccca cagaagcggg ggagagaatc attgcctgcc ttgaaccgcg tattgacgag
      361 cttgaacagg aactggaatc acttattcaa ttgaacggca cgccctccgg gaatattcgg
      421 ttatctgccg gggaacatgc cgcgcgaagt ctggtctggc cgaagctaaa accttttctc
      481 agagagtatc ccgaaatcaa tgtcgaactg gtggtggata acggttttgt cgatattgtt
      541 gaaggccgtt ttgatgccgg gatacgactg ggtgaaaatg ttgataaaga tatggttgca
      601 gtcaggattg gaccagatat gcggatggct gtggtgggag ctccggcgta ttttgctgca
      661 aaccctgcgc ctgaaacgcc tcacgagcta caaaatcatc gctgcatcaa catgcgtttg
      721 ccaactgccg gtgggcttta ccactgggag ttcgagcgag aagggaaacc gcttcgcgtt
      781 aaggttgatg ggcaactgac gtgtagtcca ttgccagaaa gaattgatgc cgcgttatca
      841 gggtttggga tcgcttgtgt ccctgaagac atggttcagg agtatataga gtcaggcaag
      901 cttattcagg tgttacagga gtggtgtcca acttttcctg ggtattatct ttactacccc
      961 agccgcaagc agcatccgcc agcttttgcg ttgttgatcg atgcgcttcg ctacaccgaa
     1021 taacgggtcc tttatcctgg acccgtttgg gtattagcgg cctacgcgtg cctgatgttc
     1081 aggcgagtaa cgttcaccga cgattttaat tgtttccagc gcctcgctta tctgctgcgt
     1141 atctttctgc gaaagaacaa tgtcagcagc ggccaggttt tcttccagcc ggtgcagttt
     1201 agtggtgcca gggatgggaa caatccacgg cttttgtgct aatagccatg ccagtgcgat
     1261 ttgtgcagac gttacccctt tctccgcagc cagttcacct aacaaggtga ccagtttttc
     1321 attggcttca atggcttgtg ctgcgaaacg cggtacggtg ctgcggtaat catctttgcc
     1381 gaaagtggtt cccggcttaa tcgcccctgt caggaaacct ttacctagtg ggctgaaggg
     1441 gacaaaacca attcccagtt cctcaagcaa cggcagaatt tcctgttcag gctcacgcca
     1501 ccacatggaa tattcgcttt gcagggcggt gacaggttgt accgcatgtg cacgacgaat
     1561 ggtttgtgct cctgcttcag acaggccgaa atgtttgacc ttaccttctg cgatgaggtc
     1621 tttcaccgtt cctgcaacat cttcaatagg aacatctgga tcaacacggt gttgatacag
     1681 caggtcaata acatctgttt tcagacgacg taatgatcct tcaacggctt cacggatatg
     1741 ttccggacgg ctgtttaaaa tctgctgctt attatcattg ccgaaggtaa aaccgaattt
     1801 agtggcaatt accacgcggt cacgaaacgg ttttaaggct tcgccgacca cttcttcatt
     1861 cagatacgga ccatacactt cggcggtatc gaagaaggtg acgccacgtt caaccgcagc
     1921 acgaataagt tcgatagcct gacgggtatc ggtcgccggg ccgtagccgt ggcttaagcc
     1981 catgcaaccg agcccaaggg cggagacttc gagtccggat ttacccagat aacgtttttg
     2041 cattgaatga atacctcttt tatcgcggct taaacatcaa gtttgcggcc agtcagccat
     2101 tccaccattg cgggatcgcg gtgagagaag aacgcgctgg ttgcggtatc cagggcggta
     2161 atttgcagca tatcttcagg gctgagttca aaatcgagaa tgttgatgtt ctcttccatg
     2221 cgttcttttc gcactgattt tgccagtgaa acaatgcctc gttggtagat ccagcgcaga
     2281 actacctggc ccacgctttt gccatacttc tcgccaattg ccgagagaac aggatgctgg
     2341 aacagaccat ttttcccttc cgcaaacggt gcccaggctt ctggctgaat gccacgactt
     2401 tgcatccagg gaacggcctg tagctgctga ttgaaagggt taacttcaac ctggtttacc
     2461 gccggaacga cgttattaaa agcgataagg tctgccagtc ggtcaggatg gaagttactg
     2521 acgccaatag cgcgaatttt accggcctgt tgcagctctt ccatggcacg ccaggctccg
     2581 tggacatcgc catatggctg gtgaatcaag tacaggtcaa cgtaatcaag ttgcagtcga
     2641 ttcagagagc gttcgaactg ggctttggcc ccttcgtaac tggtatcctg caaccagagt
     2701 ttcgtggtga caaagagttc gttacgggcg ataccgcttt gtttcagtgc gttcccgacc
     2761 tggatttcat tttgataaga ggcggcggtg tcaatcagtc gataaccgct gttaatagcg
     2821 tcaatgaccg ctctttcgca ttcggcggca tcggtcatct gaaatacacc aaagcccaac
     2881 aggggcattt caataccgtt gttcagtttt actttttgca tggtgttatc cttccgttat
     2941 cgttcatgag aagcataacg taaagggaag agctcgatta gacggcagga tttgtcaggg
     3001 gttatgaaca aaattcataa atctgtttga gggttgagat tagtcgtgca agttcgatat
     3061 ccccggaatt tacagtccga tgaataaaaa tgaatgtcca taaaaaatat attggtttat
     3121 cccggcgtat atacttactt cgacgtactc ccaata
//
LOCUS       DAZKHK010000279         3127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKHK010000279 DAZKHK010000000
VERSION     DAZKHK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.281"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(693..1293)
                     /locus_tag="ACHOS5_005197"
                     /pseudo
     CDS             complement(693..1293)
                     /locus_tag="ACHOS5_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012016515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(1382..1816)
                     /gene="uspF"
                     /locus_tag="ACHOS5_005198"
     CDS             complement(1382..1816)
                     /gene="uspF"
                     /locus_tag="ACHOS5_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP9779848.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1957..3090)
                     /gene="ompN"
                     /locus_tag="ACHOS5_005199"
     CDS             complement(1957..3090)
                     /gene="ompN"
                     /locus_tag="ACHOS5_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP9779849.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNADFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFNAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          916 a          613 c          694 g          904 t
ORIGIN      
        1 cttcagtctg aagagggctt gaatcgtgat gtcagttcga ttgacataat gttattaata
       61 atgtttacgt ttgaatgtga aatatgctta actatgcctt gattgttaat cacagggact
      121 gactatgaga aaaatactga tgtatttatg tattagcata tggattgtta tatcatgcgt
      181 gcctatcata ttaactagcg agtatttaac agaggcgagt ataatggcat tgatgtttgg
      241 tcatgccaat gcactatgta ttggggtttt tatttctatt atttatatag aatgctggag
      301 gtaaatagtg tgaacagcat tagtttgatg attttatact gaactaaagg gggaaagagg
      361 taacgaaaaa agacctccac caggtacgaa tctgaggcgg gcagatggag gtcgatatct
      421 acccgattaa attgataagt aaaagcccca tcagggattg atttgttcga gtttaattta
      481 aagtaatttc aacgatttag cggaatttct tagagttaat gattttcaag ttttcgatta
      541 ccaggaaaaa ggctgggtgc tgataaaata gtcatctgca ttccttaaat tgtgtaaggg
      601 tgaaaggata aagttcaatt taaaggcggt gcaatattag caccatctta tatatgtaat
      661 gattgatttg cctctttatt ctactgttga gattattgtt tccgaaaatt gattattata
      721 agcgcgaaaa gaaatgctac agcaaataag aggacgattg tcggcgctgg ggctctgtcg
      781 agaaaatata ataaccatac gcctgatatt gttattgata tcgttacggc gataagtaat
      841 gtcttatgaa agcgatgcgt gatgagaaca gcaatagacc ctggagctat taatagtata
      901 tagacagaat aattccgact gcttttaatg tggcaaaaat ggtaggcgat accatgcaga
      961 gtaaaccgta atgtaataat gatatctgta atccgctgac ttgtgcctgt tgatagtcaa
     1021 gacaaaaatt cctgaaaaaa cgatacctat aatggtatct ttctttatga tgctattatc
     1081 tttcagatag ccagttgcaa ttgcacaaga cagtcctaca ataaatgcac caattcccag
     1141 cggcatacct ataatccagg ctagaactat gccaggaaac attgcgtggt tcattgcatc
     1201 tcctattagc gcccagtctt ttaataccag aaaaactgat aataatgcac aggggaacga
     1261 tattataata gcgattatca atgcataatt cataaattta aattgtaatg attagagggg
     1321 aaaatgtcag cacatttgcg aaatgaatca aaaagcccgc agcaatgtgc gggcgttagt
     1381 gtcagcgcac aaccagcacg gagcactctg cgtgacgcac tacagctgcg gcgttagaac
     1441 cgagcagata agtggtgata tccggtcgat gggaagcaat gatgatcata tgagcgggga
     1501 tcttcttcgc caattccaga atgcggtctt tgggcgagcc ttcttcaaca tggacatgca
     1561 ctctgtcggt tggcagttta aattttttaa tgatctcttc cagttgcgat ttggcttccg
     1621 ctttcagatc atccattgcc ggtaattctg cggaataggc taaacccaga gaggcatagt
     1681 agggcagcga aggtattacc gtcaggaaat gaacctctgc atcatcaatc tttgcctctg
     1741 cctcaacgtg gctaatcacg cgttgagtta attctgaatc ggaaatatcg atagggacaa
     1801 gaatcgttct gttcataaaa cctcctgttt tagtatccgc ataaagtgta acgccagatg
     1861 acactttttg tgtaatgacg gagttcacat ttttaattta gatcaaagga ggaagaataa
     1921 gcagaaaaag cccgccaaaa cagcgggcag ggggatttag aactgataaa ccagacctaa
     1981 agcgacgata tcatcggtag agatgccgtt ggcagcgtag aaactgtcat cttcatccaa
     2041 caggttgatt ttatagtcaa cataggtgga catgttttta ttgaaatagt aagtcgcgcc
     2101 aacatcggcg tatttaacca gatctttatc atcaacacct gctgggttgt ctgcaccacc
     2161 ggcagcgtgc aggtcacggc ctttagacat caggaaagag actgccggac gcagaccaaa
     2221 atcaaactgg tactgtgcag tgacttcaaa gttctgggtt ttgtttgcaa cagcataatc
     2281 gctgtcgcca aacggggtca tattacgcgt ttctgaatac atggttgcca ggtaaatatt
     2341 gttagcatcg tattttagcc cagcagtcca cgcgtctgct ttatcaccac ccgccgcagt
     2401 atggttaacc tggtcattgg tgcggtcaga agaggtgtat gccgcaccag cgttaaagcc
     2461 catgcctaaa tcatatgttg tggaaagacc ccagccgtca ccgttttcat ggcgaacatc
     2521 acgtccgttg ttggtgcctt cctgaccatt actggcacct tcgttgttac cttgatactg
     2581 caccgcgaag ttcagaccat ttaccagacc gaagaaatca gcattacgat aagtcgcgac
     2641 gccattggca cgaccagtca taaagttgtc tgcattggta taagagtcac cgccaaattc
     2701 aggtagcata tcagtccagc cttcgatgtc gtacattacg ccataattac gtccgtaatc
     2761 gaaagaaccg taatctgcaa atttcagtcc ggcaaatgcc agacgggtcc atgactggtt
     2821 tgttgaagat tcggtgttgt ttgcctgaat attgtattcc cattgaccgt agccagtgag
     2881 ttgatcgtta atttgggttt cgcctttaaa acccagacga gcatagctct ggtcgccatc
     2941 tttcgctgaa ttatcagaaa aataatgcag gccatcaact ttgccataca gatctaattt
     3001 gttgccgtct ttattataaa cttcggctgc atgtgcagca cctgcagcga gcagggcagg
     3061 aattaagagt gccagtactt tgcttttcat tgaataaatc ctttagttat tttatttgcc
     3121 tttcatc
//
LOCUS       DAZKHK010000280         3124 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKHK010000280 DAZKHK010000000
VERSION     DAZKHK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.282"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            153..1364
                     /gene="bluF"
                     /locus_tag="ACHOS5_005200"
     CDS             153..1364
                     /gene="bluF"
                     /locus_tag="ACHOS5_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP9779850.1"
                     /translation="MLTTLIYRSHIRGDEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNKPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
     gene            1569..2300
                     /gene="bluR"
                     /locus_tag="ACHOS5_005201"
     CDS             1569..2300
                     /gene="bluR"
                     /locus_tag="ACHOS5_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP9779851.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTQDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2521..2925
                     /locus_tag="ACHOS5_005202"
     CDS             2521..2925
                     /locus_tag="ACHOS5_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP9779852.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2978..3103
                     /locus_tag="ACHOS5_005203"
     CDS             2978..3103
                     /locus_tag="ACHOS5_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779853.1"
                     /translation="MEIKVQRLSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
BASE COUNT          920 a          650 c          660 g          894 t
ORIGIN      
        1 acctatacag gttttttcgc caaaccgtct tcaacctttt ccacttttta tcaatgtaaa
       61 gaaacggtta tgttttggta ttaaaagaaa gacacaagaa gatatgtctg ttaccgtctt
      121 actctcacct cgcccattac cctggattgt taatgcttac cacccttatt tatcgtagcc
      181 atatacgtgg cgacgaacct gtcaaaaaaa ttgaagaaat ggtttcgata gcaaatcgca
      241 ggaacatgcg gtctgacgta acagggatct tactgtttaa tggttctcat tttttccagc
      301 ttctggaagg tccggaagaa caggttcaaa tgatatatcg ggccatatgc caggatccac
      361 ggcactataa tattgttgaa ctgttgtgcg attacgcgcc tgctcgccgt tttggcaaag
      421 caggaatgga attatttgat ttacgcctgc acgagcgaga tgacgtttta caggccgtat
      481 tcgacaaagg cacatcaaaa tttcagctaa cttatgatga cagagcgcta caattttttc
      541 gtacttttgt ccttgcaact gaacaatcaa cctatttcga gattcctgct gaagactcct
      601 ggctttttat cgctgacgga tctgataaag aacttgattc ttgtaccctt tcatcaaata
      661 taaacgacca ctttgccttt catcctattg tcgacccttt atcgcggcgg ataatcgctt
      721 ttgaagccat tgtgcaaaaa aatgaagaca gcccgtcagc tatagcggtt gggcagcgta
      781 aagacgagga aatctacaca gcggatctca aaagtaaggc gcttgcattt gcgatggcac
      841 acgcacttaa gctcggtgat aaaatgattt caatcaatct attacctatg accctggtta
      901 acaaacctga cgcagtctct tttttacttg atgaaataaa ggccaatgct ctggtacctg
      961 aacaaatcat cgttgaattt actgaaagtg aagtcatatc tcggtttgat gagtttgccg
     1021 aagcggttaa atcgctaaag gcggccggta tcagtgtagc aattgatcat tttggcgcag
     1081 gttttgctgg tttgttactc ctgtcacgct tccagcctga cagaattaaa atcagtcagg
     1141 aattgattac caatgttcat aaaagcggac cacggcaggc aattattcag gcgatcatga
     1201 aatgctgtac atcacttgaa attcaagtct ctgctatggg cgtggcaaca ccagaagagt
     1261 ggatgtggct tgaatctgca ggaattgaga tgtttcaggg agatctgttt tccaaagcta
     1321 aattgaatgg tatcccttca gttgcgtggc cggagaaaaa ataattttca gcacattatt
     1381 tcgcatgatt tctggaaatc atcgtgaaaa tgaatacgtt atgttttaat ttaatccaca
     1441 ttaaaaataa tgtttcatcc aatttagctg aaatcttatt tatactttgt acaattttag
     1501 catatattgt acaaagtatt attgggtcgt gtagaggcga cggagattta tgatcgcaag
     1561 gaggaattgt ggcttattac agcattggtg atgttgctga acgttgcggg attaatcctg
     1621 tcactctccg ggcctggcaa cgccgctacg gtttgttaaa accacaacgc agtgaaggcg
     1681 gacaccgact ctttgatgaa gaagacatac aacgcatcga agagattaag cgttggataa
     1741 gtaatggcgt tcctgtaggc aaagttaaag cattactgga aactaccagc caggatacgc
     1801 aagatgactg gaaccgcctg caagaagaga tgatgtcaat tcttcgtatg gctaatccag
     1861 ccaaactacg cgcgcgaatt atttcactgg gtcgagagta cccagtagat caattgatta
     1921 atcatgttta ccttcctgtt cgccagcgtc tcgtgcttga tcacaacacc tcccgcatta
     1981 tgagcagtat gtttgacggc gcattaattg aatacgcagc ggcctcactt ttcgaaatgc
     2041 gccgtaagcc cggtaaagag gccattctga tggcgtggaa tgttgaagag agagcacgat
     2101 tgtggctgga agcatggcgt ttatcattgt caggatggca catttctgtt cttgctgatc
     2161 ccattgaatc gccgcgcccg gaactgttcc caacgcaaac attgattgtc tggacaggca
     2221 tggcaccaac gagaaggcaa aacgaacttt tacaacactg gggtgagcaa ggttataaag
     2281 tcatctttca tgccccctaa attcaccttt atcccgcccc ttagacagaa tgttataatc
     2341 tgaacccaca ccagcgtttg tagcggagct ggtccgttgc aaaatgccca ccgggagaat
     2401 tcaggtaatc cccctttcac actttgtaca tatcacagcc aaaggacaga ccagattgaa
     2461 gtggtaaaac cgatcgtgca cactacgcag tctcacacct aacaagaggt tattatcctg
     2521 atggatcagg tcgttgtttt tcaaaagatg tttgagcaag tgagaaaaga gcaaaacttc
     2581 tcctggtttt attcagaatt aaaacatcac cgtattgcac attacattta ttatctggct
     2641 acggataaca tcagaattat tactcacgat gacacggttt tgttattaag aggaaccagg
     2701 aacctgttaa aagttagtac gaccaagaac ccagctaaaa taaaagaggc cgcattgctt
     2761 catattcgcg gaaaatctac atttcgggaa tactgttcaa cactggcagg cgctggcgtt
     2821 ttccggtggg ttactgatgt taatcataac aaacgcagtt actacgccat tgataatacg
     2881 cttttatata ttgaagatgt agaaaataat aaaccattaa tctagctaaa gttgggagct
     2941 taaaaaatgc ttcataattc agtaaggcat tagcataatg gaaataaaag tgcagagact
     3001 atccctatgg atgattaata ctgtcttttt attgctcccc ataaataatc accagaccaa
     3061 tacgatcaaa ttgatatttg aaatgtgttc acttgacttt tgatacgtta ttttataacg
     3121 gtta
//
LOCUS       DAZKHK010000281         3111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKHK010000281 DAZKHK010000000
VERSION     DAZKHK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.283"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            22..345
                     /locus_tag="ACHOS5_005204"
     CDS             22..345
                     /locus_tag="ACHOS5_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779854.1"
                     /translation="MGRNMSAARTDGFIRNIHSRNPFDVIRADVVISRLEKQAHWGCG
                     LHYEIYEANLFDMAMNHLSRLPLKDRPVFIGAAARKGLFLTMEETRLAREAKDALMAE
                     LALEY"
     gene            404..595
                     /locus_tag="ACHOS5_005205"
     CDS             404..595
                     /locus_tag="ACHOS5_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779855.1"
                     /translation="MGYITYVTDQRPGEPDILTGNTFADLEIRDSDGHLLLKVSAPEA
                     GWTHESLNLVQPQEVQEGK"
     gene            674..1348
                     /gene="tnpA"
                     /locus_tag="ACHOS5_005206"
     CDS             674..1348
                     /gene="tnpA"
                     /locus_tag="ACHOS5_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9779856.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPECAPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
     gene            1345..1692
                     /gene="tnpB"
                     /locus_tag="ACHOS5_005207"
     CDS             1345..1692
                     /gene="tnpB"
                     /locus_tag="ACHOS5_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9779857.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFSKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            1712..>3111
                     /locus_tag="ACHOS5_005208"
     CDS             1712..>3111
                     /locus_tag="ACHOS5_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9779858.1"
                     /translation="MNNTLPDDIEQLKALLIAQQAVIVRLSGEITGYAREISSLRALV
                     AKLQRMLFGRSSEKSREKIEKKIARAETRITELQNRLGEAQLQLTSMAGETAPKTSDS
                     PVRKAFPATLPRDRQVISPAETECPVCSGKLKPLGESISEQLDIINTAFRVIETVRPK
                     LACSRCDCIVQAPQPPKPIERSYASPALLARIIMAKFAEHLPLYRQSEIYARQGVELH
                     RNTMGRWVDIMGEQLRPLYDELKHYVLMPGKVHADDTPVNVLEPGQGKTRTGRLWVYV
                     RDDRNAGSTMPAAVWFSYSPDRKGIHPQQHLADYRGILQADAYAGYNALYESGQVTEA
                     ACMAHARRKIHDVHVRHPTTVTGEALRRIGALYAIESEIRGSPAEERLAVRKARTVPL
                     MQSLYEWLQGQMSTLSRHSDTAKAFTYLLKQWDALNEYCRNGWVEIDNNLCENALRVV
                     ALGRRNYMFFGSDGGG"
BASE COUNT          788 a          806 c          862 g          655 t
ORIGIN      
        1 aaaaacagag gtgtattaaa gatgggcaga aatatgtcag cagcacgtac cgatggcttt
       61 attcgtaata ttcattcccg caatcccttt gatgttatcc gggctgatgt ggtgatcagc
      121 aggctggaaa agcaggctca ttggggatgt ggtctgcact atgaaattta tgaagcgaat
      181 ttgtttgata tggcgatgaa tcatctttcc cggcttcctc tgaaggatcg tccggtattc
      241 attggtgctg ctgcacgaaa ggggcttttt ctgacgatgg aagaaaccag gctggcacgg
      301 gaagcgaaag atgctctgat ggctgaactt gctctggaat actgaaaatc gggccgcacc
      361 gtcgtcccgg atagtgctga aatatttaat gtggagagaa tggatgggat atatcacata
      421 tgttacagat caacgacccg gtgaaccaga tatcctgacc gggaacacgt ttgctgatct
      481 ggagattcgt gattctgatg ggcatcttct gctgaaagtg tctgcacctg aagccggctg
      541 gacacacgaa agcctgaacc ttgttcagcc gcaggaagtg caggaaggga agtaagcgcc
      601 acatctgcga cgtcttgtga aaattgtcct gtctggcaac aatcgcgccc atctatatag
      661 atggacacga acgatgaatt cccagacaaa aaaagatatt ccctgcttcc gttcttattt
      721 gcctgatgct ctgcgtttaa gatttgaaga taaactgacc atccgggcca tcgctcagcg
      781 tctaggtctc agtcattcca caatacatac gctttttcag cgatttattg catccggtat
      841 cgcatggcca ttgcccgatt cagtttcatt agctcagctt gacgccatcc tttatgccaa
      901 cagaaagaag gaattaacag agcctcaaat cagcgaaggc acatggcgaa aagaacggcg
      961 agccagctac agccgtgaat ttaaggtccg tctggctaag caggcattac agcccggtgc
     1021 tgttgttgcc cggatcgcca gagagcacgg tatcaatgat aacctgctgt ttaaatggaa
     1081 aagccagtac gaggacggct tactgagcga tgatgacata caggaatgca tgcctgtccc
     1141 ggtggctctg actgatacgc cagagccgac cagaccagtt acaaatccct tctggcgtaa
     1201 caagcctgat gagtgccctg agtgtgctcc cggaaacgtc ccacggtgcg agctgcatct
     1261 taaatcaggt gtggtaaaac tgtttgaccc tctcactccg gaaatgttac gggcgctaat
     1321 ccgcgaaatg aaaggaggta cccgatgata acgctgccaa ccggtaccag aatctggatc
     1381 atcgctggca tcacagatat gcgttgtggc ttcaacggcc tggcttcgaa ggtgcagaac
     1441 acgctgaaag atgacccgtt ctccgggcat atcttcgtct tccggggccg cagtggcaaa
     1501 atggtgaaaa tactgtgggc cgatcgtgac gggttatgcc tgttctccaa acgcctggaa
     1561 cggggccgct tcgtctggcc ggtaacccgg gaagggaaag tgcacctgac gccagctcag
     1621 ttatccatgc tactggaggg gatcgcgtgg caacatccca aacggacaga acggcctggc
     1681 atccggatat aacccgtgat aaaacagggg aatgaacaac acactccccg acgacatcga
     1741 gcaactgaag gccctgctga tcgcacagca ggcggttatc gtccgtctgt ctggtgaaat
     1801 aaccggctat gcccgcgaga tcagctcgct cagagcgctg gtcgctaaac tgcagagaat
     1861 gctgttcggt cgcagcagcg agaaaagccg cgagaagata gaaaagaaga tcgcacgggc
     1921 agaaacgcgt ataaccgagc tccagaacag gcttggtgag gcgcagttgc aactcacctc
     1981 aatggccgga gagacagcgc cgaaaacatc agactctccc gtccgcaaag catttccggc
     2041 aacacttccc cgtgacaggc aggttatctc cccggcagaa accgaatgcc ccgtctgcag
     2101 cggcaaactg aaaccgctgg gagaaagcat ctctgaacaa ctggatatca tcaacaccgc
     2161 gttcagggta atcgaaacgg ttcgcccaaa actggcctgc agccggtgcg actgtatagt
     2221 tcaggctccg cagccaccaa aacccatcga gcgcagttac gccagtccgg ctctgctggc
     2281 ccgcataatc atggctaagt tcgccgagca tctgccgctg taccgtcagt cggaaatcta
     2341 tgcccgccag ggcgtggagc tgcaccgcaa tacgatgggg cgctgggttg acatcatggg
     2401 agagcagctt cgcccgctgt atgatgaact gaagcactat gtgctgatgc cgggtaaagt
     2461 gcatgccgat gacacgccgg taaatgtact ggagccgggt cagggtaaaa cccgtaccgg
     2521 acggctgtgg gtctatgttc gtgacgatcg caacgccggt tcgaccatgc cggcagcggt
     2581 gtggttctca tactctcccg accgcaaagg tatccacccg cagcaacatc tggcggacta
     2641 cagaggtatc ctgcaggccg atgcatatgc gggttacaat gctctttacg aaagcggtca
     2701 ggtaaccgaa gcggcttgta tggcacatgc ccgacgcaag atccacgatg tacatgtccg
     2761 ccatccaacg acagtaacgg gagaagcact ccgtcgtatc ggggcactgt acgctatcga
     2821 gtctgagatc cgcggcagtc cggcagaaga gcgactggcg gtcagaaaag ccagaacggt
     2881 accgctaatg cagtcgttgt atgagtggct ccaggggcag atgagcacgc tgtcgcgtca
     2941 ctctgatacg gcgaaagcgt tcacctatct gctgaagcaa tgggacgccc tgaacgaata
     3001 ctgccgcaat ggctgggtgg agatcgacaa taatctgtgt gaaaacgccc tccgggtagt
     3061 tgcactgggg cggcgtaact acatgttctt cggctctgat ggaggaggcg a
//
LOCUS       DAZKHK010000282         3111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKHK010000282 DAZKHK010000000
VERSION     DAZKHK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.284"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2426
                     /gene="vat"
                     /locus_tag="ACHOS5_005209"
     CDS             <1..2426
                     /gene="vat"
                     /locus_tag="ACHOS5_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vacuolating autotransporter toxin Vat"
                     /protein_id="HFP9779859.1"
                     /translation="LKTSSYGWFPTGQVSNEHWEYVGHDQNSAQALLANRINNKGYLY
                     HGKLLGNINFSNKATPGTTGALVMDGSANMSGTFTQENGRLTIQGHPVIHASTSQSIA
                     NTVSSLGDNSVLTQPTSFTQDDWENRTFSFGSLVLKDTDFGLGRNATLNTTIQADNSS
                     VTLGDSRVFIDKKDGQGTAFTLEEGTSVATKDADKSVFNGTVNLDNQSVLNINDIFNG
                     GIQANNSTVNISSDSAILGNSTLTSTALNLNKGANALASQSFVSDGPVNISDATLSLN
                     SRPDEVSHTLLPVYDYAGSWNLKGDDARLNVGPYSMLSGNINVQDKGTVTLGGEGELS
                     PDLTLQNQMLYSLFNGYRNTWSGSLNAPDATVSMTDTQWSMNGNSTAGNMKLNRTIVG
                     FNGGTSSFTTLTTDNLDAVQSAFVMRTDLNKADKLVINKSATGHDNSIWVNFLKKPSD
                     KDTLDIPLVSAPEATADNLFRASTRVVGFSDVTPTLSVRKEDGKKEWVLDGYQVARND
                     GQGKAAATFMHISYNNFITEVNNLNKRMGDLRDINGEAGTWVRLLNGSGSADGGFTDH
                     YTLLQMGADRKHELGSMDLFTGVMATYTDTDASAGLYSGKTKSWGGGFYASGLFRSGA
                     YFDLIAKYIHNENKYDLNFAGAGKQNFRSHSLYAGAEVGYRYHLTDTTFVEPQAELVW
                     GRLQGQTFNWNDSGMDVSMRRNSVNPLVGRTGVVSGKTFSGKDWSLTARAGLHYEFDL
                     TDSADVHLKDAAGEHQINGRKDGRMLYGVGLNARFGDNTRLGLEVERSAFGKYNTDDA
                     INANIRYSF"
     gene            2582..3076
                     /locus_tag="ACHOS5_005210"
     CDS             2582..3076
                     /locus_tag="ACHOS5_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9779860.1"
                     /translation="MNNIDLLKTITNYKKIKNPAYPAQESLLIHLYIRVNDKIQSIIE
                     NELSEYRINTSTFMVLVSLYMSDDYCQSPSDIYKELQFSKTNITHIIDKLEKKNIAKR
                     INNKNDRRSKSICLTPDGVTLAQKLINTQNVMLKKIWSGLSDDEMKTFELANKKLLSN
                     LNVN"
BASE COUNT          926 a          685 c          743 g          757 t
ORIGIN      
        1 tcctgaaaac aagtagttat ggctggttcc ctaccggtca ggtcagtaac gagcactggg
       61 aatatgtcgg acatgaccag aacagtgcac aggcgctgct tgcaaacaga attaataata
      121 aagggtatct gtatcatggc aagttgctgg gaaatattaa tttctcaaat aaagcaaccc
      181 cgggtacaac cggcgcattg gttatggacg gctcagcgaa tatgtccggt acatttactc
      241 aggaaaacgg tcgtctgacc attcagggcc acccggttat ccatgcttca acgtctcaga
      301 gtattgcaaa tacagtctcg tctctgggcg acaattccgt tctgacacag cccacctcat
      361 ttacacagga tgactgggag aacaggacgt tcagctttgg ttcgctcgtg ttaaaagata
      421 cagactttgg tctgggccgc aatgccacac tgaacacaac catccaggcc gataactcca
      481 gcgtcacgct gggcgacagt cgggtattta tcgacaaaaa agatggccag ggaacagcct
      541 ttacccttga agaaggcaca tctgttgcaa ctaaagatgc agataaaagt gtcttcaacg
      601 gcaccgtcaa cctggataat cagtcagtgc tgaatatcaa tgatatattc aatggcggaa
      661 tacaggcgaa caacagtacc gtgaatatct cctcagacag tgccattctg gggaactcaa
      721 cgctgaccag taccgccctg aatctgaaca agggagcaaa tgctctggcc agtcagagtt
      781 ttgtttctga cggtccagtg aatatttctg atgccaccct gagtctgaac agccgtcctg
      841 atgaggtatc tcacacactt ttacctgtat acgattatgc cggttcatgg aacctgaagg
      901 gagacgatgc ccgcctgaac gtggggccgt acagtatgtt gtcaggtaat atcaatgttc
      961 aggataaagg gactgtcacc ctcggagggg aaggggaact gagtcctgac ctgactcttc
     1021 agaatcagat gttgtacagc ctgtttaacg ggtaccgcaa tacctggagc gggagcctga
     1081 atgcaccgga tgccaccgtc agcatgacag acacccagtg gtcgatgaac ggaaactcca
     1141 cggcaggaaa tatgaaactt aaccggacaa tagtcggttt taacggggga acatcatcgt
     1201 tcacgacact gacaacagat aatctggacg cggttcagtc agcatttgtc atgcgtacag
     1261 accttaacaa ggcagacaaa ctggtgataa acaagtcggc aacaggtcat gacaacagca
     1321 tctgggttaa cttcctgaaa aaaccctctg acaaggacac gcttgatatt ccactggtca
     1381 gcgcacctga agcgacagct gataatctgt tcagggcatc aacacgggtt gtgggattca
     1441 gtgatgtcac ccccaccctt agtgtcagaa aagaggacgg gaaaaaagag tgggtcctcg
     1501 atggttacca ggttgcacgt aacgacggcc agggtaaggc tgccgccaca ttcatgcaca
     1561 tcagctataa caacttcatc actgaagtta acaacctgaa caaacgcatg ggcgatttga
     1621 gggatattaa cggcgaagcc ggtacgtggg tgcgtctgct gaacggttcc ggctctgctg
     1681 atggcggttt cactgaccac tataccctgc tgcagatggg ggctgaccgt aagcacgaac
     1741 tgggaagtat ggacctgttt accggcgtga tggccaccta cactgacaca gatgcgtcag
     1801 caggcctgta cagcggtaaa acaaaatcat ggggtggtgg tttctatgcc agtggtctgt
     1861 tccggtccgg cgcttacttt gatttgattg ccaaatatat tcacaatgaa aacaaatatg
     1921 acctgaactt tgccggagct ggtaaacaga acttccgcag ccattcactg tatgcaggtg
     1981 cagaagtcgg ataccgttat catctgacag atacgacgtt tgttgaacct caggcggaac
     2041 tggtctgggg aagactgcag ggccaaacat ttaactggaa cgacagtgga atggatgtct
     2101 caatgcgtcg taacagcgtt aatcctctgg taggcagaac cggcgttgtt tccggtaaaa
     2161 ccttcagtgg taaggactgg agtctgacag cccgtgccgg cctgcattat gagttcgatc
     2221 tgacggacag tgctgacgtt cacctgaagg atgcagcggg agaacatcag attaatggga
     2281 gaaaagacgg tcgtatgctt tacggtgtgg ggttaaatgc ccggtttggc gacaatacgc
     2341 gtctggggct ggaagttgaa cgctctgcat tcggtaaata caacacagat gatgcgataa
     2401 acgctaatat tcgttattca ttctgatgct gtgcagcaaa gggactattg ctttgtccct
     2461 ttgctgcgat tatctgatta cggtacaggc tggaacttag attttccggc gttcacgata
     2521 cttcatgtaa cactcaggtt gagtaatctt catgagtttt cttttgccgt gtggtggaat
     2581 tatgaataat atagatttat taaaaactat aacaaactac aaaaaaatca aaaatccggc
     2641 atacccggct caggagagtc tgttaataca cttatatatt agagtaaatg acaaaatcca
     2701 gagtattata gaaaatgaat tgagcgagta tagaataaac acttcaacat ttatggtgtt
     2761 agtttcatta tatatgtctg atgattattg tcagtcccct tctgatattt acaaggagtt
     2821 acaattctcc aaaacaaaca tcacacacat catagacaaa ttagaaaaaa agaatattgc
     2881 caaaagaatt aataataaaa acgatcgtcg aagcaaaagt atttgcttaa cccctgacgg
     2941 agttacactt gctcaaaaac taataaacac tcaaaatgta atgctaaaga aaatctggag
     3001 cgggctatca gatgatgaaa tgaaaacatt tgaactcgcc aacaaaaaat tattatcaaa
     3061 ccttaatgtt aattgatgtt aattgacgtt aaaacatact aacagtcatc t
//
LOCUS       DAZKHK010000283         3076 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKHK010000283 DAZKHK010000000
VERSION     DAZKHK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.285"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..453)
                     /locus_tag="ACHOS5_005211"
     CDS             complement(<1..453)
                     /locus_tag="ACHOS5_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFP9779861.1"
                     /translation="MQHRQDNLLASRTSLPGMVSGQCAFKLRTFSPVARYFSLLPCLC
                     ILSFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALKRSAPLTVIPSPEMSAGTEGP
                     CFTVSRIVVRGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYI"
     gene            complement(822..1058)
                     /locus_tag="ACHOS5_005212"
                     /pseudo
     CDS             complement(822..1058)
                     /locus_tag="ACHOS5_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1155..1778)
                     /locus_tag="ACHOS5_005213"
     CDS             complement(1155..1778)
                     /locus_tag="ACHOS5_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9779862.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIATDVLRLRKAGMPVVTTTVEVHDNLTGTTRKIPAYYL"
     gene            complement(1867..2379)
                     /locus_tag="ACHOS5_005214"
     CDS             complement(1867..2379)
                     /locus_tag="ACHOS5_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779863.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGYING
                     QQIQTFCSSFHRRVHERITERNHTACPSSPVVLRWLREQHRGATMRCLLLLSQTSICH
                     PRASVTVDEACSQVADLLQQTWPVISAGGQCRVEGCFRVVRPDTSEQYVALKTAVQSL
                     MPRGIATIIR"
     gene            complement(2660..2838)
                     /locus_tag="ACHOS5_005215"
                     /pseudo
     CDS             complement(2660..2838)
                     /locus_tag="ACHOS5_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
BASE COUNT          797 a          796 c          751 g          732 t
ORIGIN      
        1 tatatagccg tccgtcacgg catccgtgac cgcggtcagc cccgtgatat tcagacactg
       61 attcacccac ggtgccacca gtctgtcggt ttctgcagac gtcagtcggg tggccccacg
      121 gacaacaatg cgtgacaccg taaagcaggg accttcagta ccggcagaca tttccggaga
      181 cggtatgaca gtcagcggcg cactgcgctt cagcgcatca cgctggcgct ggttttcatc
      241 cagcaactgt tgctgttgct gctgaattgc actgcggtca cccggagaca gcatggctgc
      301 cggagacgaa aacgaaagaa tacaaaggca ggggaggagg gaaaaatagc gtgccaccgg
      361 agagaaagtg cggagcttaa atgcgcactg accggaaacc ataccaggca acgacgttct
      421 gctcgccagt aagttatcct gccggtgctg catattctcc tcgtggggta ttcagtgtaa
      481 attaacgtaa gcggaatgtc agtgctgaaa tttacaccag ataaaaaaca tacagaaaca
      541 atcctgttat gtaaaatctg gaaaaaatgt cagaaatgtg atcgaagttg tgttttacgg
      601 aggggagaaa ggaaaacagt catcctttga tggtaaaaaa gatgtttcct gcagagtaac
      661 aatcagggat gcaacacaag ttccagttca ggtgttgtgg atttgagtaa aaaaacaatc
      721 gtaactctca ggagaaccgg agattccccg aagcggcgta ttcttataaa tggaaatccc
      781 tgcaggaaat cacgccgcgc tatagcagca taatccactt tttaattctc atccccggag
      841 acaagcccgg aaaaccactc tgggtgttca ctgtatgccg tgttcagcgc atcccaggaa
      901 agttcatata aaagctgtcc tgcaatttac gggtaaccac ctctgccaac cggttccggt
      961 tatccgccag tccggcataa cctctctctg ccagctattc cttcattcag ttcatgttct
     1021 atagacagat tgataaaatc gtaatcagcc cctagcattc ccgtttcctt tgcacacata
     1081 ttcaggcacg gggataaagt ataaagaatg tcgtactgct gctaccagag caatattccc
     1141 ccctgatggc cgtatcagag atagtatgcc ggtattttgc gggtggttcc cgtcaggtta
     1201 tcgtgtacct ccacggtcgt agtcaccacc ggcattccgg cctttctcag cctcaaaaca
     1261 tcagttgcaa tacgctgact gccgaaccag aacaggccgt ccagtgcagt caccagcaac
     1321 cccgcctcca gcgcatgctt cagccgttca cggggcgctt tcacttcccg ggcaatctgc
     1381 tggtatggcg atgatgtgtt ttcattccca atcacccggc gaatacgatg agacagatga
     1441 taccggtatg tatccggcac accggaaagg ctggccttca ggctgtacac gcagccaaat
     1501 cgtttatcat tgaacaccac atttttctgg ctgatgcccc attcttcacg cagcgcggca
     1561 atcagttgtg gtgtacgggt aagcaacaag cgaaaaggca gttcaaaact ggtgacataa
     1621 tccacattca acagggcaat gcgaagtcgt tcttctggtc cggcttctgt ctgccggcac
     1681 tcctccagga catcctgcca ctgcaggcga agacgggaag actcattcag ttctgtaaag
     1741 cagtatttat ccgccagata gtcaattcgt gtatgcatac tgaagagtat tccgtataaa
     1801 gattcaactg gcaaaacttt atcagtctgt aaaaactaac ggaagagtcg atatttctcc
     1861 cgacaatcac cggatgattg ttgcaatacc ccgtggcatc agagactgaa cagcagtttt
     1921 taacgcaacg tattgctctg atgtatcagg ccggacaacc cgaaaacagc cttccacccg
     1981 gcattgtccg ccagcgctta tcaccggcca ggtctgttgc agtaaatccg ccacttgcga
     2041 acatgcttca tcaactgtga cactggcccg cggatggcaa atgctcgtct ggctgagcag
     2101 caacaggcat cgcattgttg ctcctctatg ttgttcccgc aaccagcgta ataccaccgg
     2161 cgaggatgga caggcagtgt gattacgctc cgtaatacgt tcgtgcaccc gtcggtgaaa
     2221 ggaactacag aatgtctgaa tctgttgccc gttgatgtat ccttctgtcg aatgaagtgt
     2281 gaagtggatt gccagcagat gcggccagtg atccaccgcc tgctgaacaa aacgccggat
     2341 ttcccccggc tctgaaagta aggcttcggt tatttgcact attttatctc tgttgaattt
     2401 ggttaagtcg gtgcagacgc atcaacacaa gtacggttcg atgcaaacag ctgtgactgg
     2461 caatatgaaa ggaatgatga atcagtcagg atgacaaagt gccggctgac cggaggggac
     2521 gcaggaagat tcacggggga accagcacca gggaacagcg ccacaatacc agcgctgaca
     2581 cgttgaacat tgccagcgta ccggtatcac aacacgtttc atacttctgc ccccgtgatt
     2641 cttcgattcg ttactgtatc tactgtgaca cttcgctttt atacctgcgg ctggatcggc
     2701 ccggcttgat gaatcttcac tgatcagctt ataaaaccct ctgtcggtca taccggtgaa
     2761 actggtgata tagttcatgt caatcaggga attatcggca cgcagaaata cgctgtcgtg
     2821 gcttgttgta gtcaacatgg tcagaatgtc ctctgtgaga tttatgaaga ttgtgcgaat
     2881 gcggggaatc tactgagctg tgctttcaga actggcctgt tacgggatac cagggattac
     2941 cggcggggta acgggcttcc ggatcataca caccacgatt atcgcggaca aaatcactga
     3001 acgcccatat cacctcttta agtatgtctt cgcagcccgg tacatgacga tccagcgcca
     3061 catcccgagt ggtact
//
LOCUS       DAZKHK010000284         2969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKHK010000284 DAZKHK010000000
VERSION     DAZKHK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.286"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..911
                     /locus_tag="ACHOS5_005216"
     CDS             <1..911
                     /locus_tag="ACHOS5_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9779864.1"
                     /translation="KLREKIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKAS
                     SRFMQSLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQ
                     ILEKTNIPTINVVGSPFSPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMENRL
                     QRQQLNGWHKAMLSHYQNTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEI
                     ALGIMFECQRCLLKIPGNIAVACLDGSDSCDQTHPTLTSIRIDYKKMGIETGKLLIGL
                     LNNNHDESEESRIVQFNYQIELRQST"
     gene            1666..2289
                     /locus_tag="ACHOS5_005217"
                     /pseudo
     CDS             1666..2289
                     /locus_tag="ACHOS5_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313589.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            2511..>2969
                     /locus_tag="ACHOS5_005218"
     CDS             2511..>2969
                     /locus_tag="ACHOS5_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFP9779865.1"
                     /translation="MQHRQKNILTKTSLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     CFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSSIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYI"
BASE COUNT          766 a          709 c          743 g          751 t
ORIGIN      
        1 acaagctgag ggaaaagata gaacaggccg tagaagagtt gggttatatt cccaacagaa
       61 ctgctggcgc ccttgcatct ggtcacagtg atacagtcgc cgtactggtt ccttccctga
      121 cagataaagc cagctcccgc tttatgcagt cgttgcagca ggtgcttaat aaaaatgaat
      181 tccagctact gcttgggtgt catgagtata atcagctcaa agaagctgaa attctgatga
      241 cgcttcttca aggcaacccg gcggcgttag ttatttttgg ttctcagttg gctgataaaa
      301 ctcaccagat actcgagaaa acaaatatac ctaccattaa cgttgtcggt tcccctttca
      361 gtccggctca aattaccatc gaaacagcat tttttgaagc atcacataaa ctgaccgaac
      421 atctgctgga gcagggttat aagaatatcg gatttatcgg tgcgcatatg gaaaaccgcc
      481 tgcaacgtca gcaactgaac ggctggcata aagcaatgct gagccattac cagaacactg
      541 atcttgttat cacaatgccg gatgccgcaa gtctgcaatt ggggcgttac gcattaaacg
      601 agatattact gcgacaacca gagctggatg ctgtgatttg cagccacgaa gagattgcgc
      661 tgggtatcat gtttgagtgt cagcggtgtt tgttaaaaat ccctggaaat atcgctgtag
      721 cttgtcttga cggttcagac agttgcgatc aaacacaccc aaccttgaca tccatccgta
      781 ttgactacaa aaaaatgggg atagagacgg gtaagttact gattggacta ttgaataata
      841 atcatgatga atcagaagag tcgcgtatcg tgcaattcaa ctaccagata gaattacgtc
      901 agagtacatg agcctaaaag ggcactattg caaagttagc tattgtggta gggatagccg
      961 tttccggcta ttccccgcgc agatccggat gtgtgctgct aacgcatcca ttcctacttc
     1021 aggtatttgg cgtagaagcg gatgctaaaa acttgccgta aaaccgcaat atcagcccgc
     1081 agtgtcgctc tccagattat ccattcctat attgatgaca acgacacgta agactgtgac
     1141 tcaccaaact tggatgtttt tctgagctta aagctcgttg cgtgaataag gcgagagtca
     1201 gcgaatctca tcgaggcccg cagcaccggc tgcaataggg atatagagca gcaatctacc
     1261 gtcaacgtca aaaacacgga agccttgcaa acgggatgcg ttcatataaa caacatggag
     1321 cagcaacaat actatgcctg ttgttgctca gtcaggacct tttctgccat gctcgagcca
     1381 gcgtcacgat tgatgaagaa tgttctcaac tgattaattt actgcaacag acgtggctgg
     1441 taataagcgc tggcggacaa tgcagggtga aaggctgttt tcgggttgtc cggcctgata
     1501 catcagagca atacgttgcg ttaaaaacgg ctgttcagtc tctgatgcca cggggtattg
     1561 caacaatcat ccggtgatgg acgggagaaa tatcgactct tccgttagtt tttacagact
     1621 gataaagttt tgccagctga atctttatac ggaatgctct tcagtatgca tatacgaatt
     1681 gactatctgg cggataaaaa ctgctttaca gaactgaatt aatctccacg ccttcgccgg
     1741 cagtggcagg atgttctgga ggagtgccgg cagacagagg ccgggccaga agaacggctg
     1801 cgtattgctc tgctgaatgt ggattacgtc accagttttg aactgccttt tcgcttgttg
     1861 cttactcgta caccacaact gattgccgcg ctgcgtgaag aatgggacat cagccagaaa
     1921 aatgtggtgt tcaacgataa acgctttggc tgcgtgtaca acctgaaggc cagcctttcg
     1981 ggtgggccgg atacattccg gtatcatctg tctcaccgta ttcggcgggt ggttgcgaat
     2041 gaaaacacat catcgccata ccaactgatt gcccgggaag tgaaagcgcc ccgtcaacgg
     2101 ctgaagtatg cgctggagac aggattgcag gtgagcgcac tggacggcct gttctggttc
     2161 ggcagccagc gcattgcggc agatatcctg aggctgagaa agtacgggat gcggattgcg
     2221 acggcttctg tggacgtgta tgacaacata accggaacaa ctcgtcatat ccctgtatat
     2281 tactcctgac gcaaaatagt ctgtgggggc cggagcatga ccggtaaccg gaacagggtt
     2341 ttgccctcgt cacagccaaa aatacgtcct ttatcacatt tcttcgattt ttcctgatat
     2401 tttaccgggt tactgttcaa aaatgcttct cttctgatgt aaatttcttt actgacactt
     2461 tttgacatga atttacactg gcatcacaca ggccagaata cggggagaac atgcagcacc
     2521 ggcagaaaaa cattctgacg aaaacgtccc ttttatcccg tgcgttgtct gtcccctgtt
     2581 gtgatatgtt ccggcgcggc tctccgtgga tatgctatct ctccctctcc gttttttctg
     2641 gttgtttcat ccccgcattt tcgtctccgg cagccatgct gtctccgggt gaccgcagtg
     2701 caattcagca gcaacagcag cagttgctgg atgaaaacca gcgtcagcgt gatgcgctgg
     2761 agcgcagtgc gccgctgacc atcacgccgt ctccggaaac gtctgccggt actgaaggtc
     2821 cctgctttac ggtgtcaagc attgttgtca gtggggccac ccgactgacg tctgcagaaa
     2881 ccgacagact ggtggcaccg tgggtgaatc agtgtctgaa tatcacgggg ctgaccgcgg
     2941 tcacggatgc cgtgacggac ggctatata
//
LOCUS       DAZKHK010000285         2927 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKHK010000285 DAZKHK010000000
VERSION     DAZKHK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.287"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(939..>2927)
                     /locus_tag="ACHOS5_005219"
     CDS             complement(939..>2927)
                     /locus_tag="ACHOS5_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9779866.1"
                     /translation="TLNLDNAAFSAGRNATVEGDIISTSSDIILGDKTAYIDVHSGKN
                     ITNQGFTFRQQISTGESIGETAFSGQIISTGGSVKVGDKATVTLTKSSSLDGTDLTIE
                     KGGVMTAQGGLFTSKETSIAGKLNLTGTPGQNDVWSSAIYLGYGGYNLSEDNAVFTAK
                     NQAAVTADIKSDKEATISLGQEAKTDNQNSPVYSAFTLDALNGFDTSLTGKINAAKST
                     LTMNDALWKVTGDSALKNMSTTGSMVLFSNENRNSFNKLTVDELTTNNSAFVMRTNTQ
                     QTDQLIVKNKLEGANNLLLVDFIEKKGNDKNGLNIDLVKAPENTSKDVFKTETQTIGF
                     SDVTPEIKQQVKDNNSVWTLTGYKTVANASAAAKATSLMSGNYKAFLNEVNNLNKRMG
                     DLRDINGEAGAWARIMSGAGSAGSGYSDNYTHVQIGADKKHELDGLDLFTGLTMTYTD
                     SHAGNHDFSGETKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYST
                     HSWYAGAEAGYRYHVTEDAWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDKDYNPLIG
                     RTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMSV
                     GLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT          706 a          683 c          705 g          833 t
ORIGIN      
        1 tttttttctg cgcgtaatct gctgcctgta aacgaaaaaa accgccttgt ggcgggttct
       61 tccgtccgat cagaagtctt ctgacctctt ctaatcagcg gtggtaactg gctatcgcag
      121 agcataggta cctaaattgt acttctagtg tagtcaaaat cagactgata cttcaagaac
      181 tctgttacca acttataaat ctcgctctgc taacccggtt accaatggct gctgccagtg
      241 acgttaagtc gtgtcctttc caggttggac ccaagagaac agttaagggt ttggggagta
      301 gtggaactca aaaggcggtt aagcggttca attgaatggt caataacagg taaatcaatc
      361 tgttgagatc aacaacgtct tttgctccgc aaacggccag gatgagtgaa gccaacttgg
      421 ctcctcccca aaacagatag ccaagaacgc agattcacga tgtaaattgg ctggttgatt
      481 tttggctatt taaaatgacc gcttaacggc ggttattgtt ccattggccc tcaaacccca
      541 aaattatctg cggggttctg cagataatgt tttttgctcc ataacaggct gaaaaggtgg
      601 gcacctacgt taccaatact ggcttaatgg ctacatacgg cagtcggttt acgcttacgt
      661 tctctgtgac agttgtgtca cacatgaagg gggaatgtaa cattcagaat cttactgaaa
      721 atgtgccatt tgtgggcacc ggtgatcaat cctgtatgca ggatttgtat cacagttccg
      781 gatgagatga agacgtgtgt ggtcgtaaaa aagccggcat tactgccagc tttagtcatc
      841 gcagttaagt aaacaaatcg actgataccc cggaaatgta taccagggcg gcaggtcctg
      901 agcataaagg gcccgcaggc ccttttgaat acggagtatc agaacgagta acggaagtta
      961 gcgttgacag cattatcaac gttgtactta ccaaaggcgg atttctcaaa ctccagtcca
     1021 aagcggacgt tgtccctgat ttctgcattc aggccaacgg acatcagcat acggctgtct
     1081 ttttcacctt tgatacgttt ttcacctgat gcgtcccgca atacggtttc gccgttagcc
     1141 agcaggtcga actggtagcc cagaccggca cgggctgtca ctttccagtc cttacctgag
     1201 aaggatttac ccacatccac accggtacga ccaatcagcg gattgtagtc tttgtccttc
     1261 atggacagat gcatcccctg gtccttccat gcaaactgtt taccggatac ggcaccgtaa
     1321 accagttctg cctgtggctc aatccaggcg tcctcagtga catgatagcg gtagcccgct
     1381 tctgcaccgg cataccatga atgtgtgctg taatcacggg ttccgagtcc ggcaaaggtt
     1441 gcggtgtact cattatcatg gtgaacatac ttaccaatca ggtcgatata ggcaccggaa
     1501 tcaaacatgg cagaagcgta gagacctgcc ccgacagatt tggtttcacc ggagaaatca
     1561 tggtttccgg cgtggctgtc ggtataggtc atggtcagac cggtgaagag gtcaaggcca
     1621 tcaagttcat gttttttatc cgcaccaatc tgtacatggg tgtagttatc agagtaaccg
     1681 ctaccggcag agccggcgcc gctcatgata cgtgcccatg caccggcttc accgttgatg
     1741 tcacggaggt cacccatacg tttgttgagg ttgttgacct cattcaggaa ggccttatag
     1801 ttgccggaca tcagagatgt tgcttttgct gcagcactcg cattggcgac agtcttatag
     1861 ccagtcagcg tccatacgga gttattgtct tttacctgtt gtttgatttc aggcgtcaca
     1921 tcactgaaac caatggtctg tgtttcagtt ttgaatacat ctttactggt attctccgga
     1981 gccttaacca ggtcgatgtt cagaccgttt ttgtcgtttc cttttttctc aatgaaatcg
     2041 actaacagca ggttgtttgc accttccagt ttgtttttaa caattaactg gtcggtctgt
     2101 tgtgtattgg ttcgcatcac aaaggcgctg ttgttggtgg tcagttcatc aacagtcagc
     2161 ttattgaatg agttacggtt ttcattactg aagagaacca tactgccggt tgttgacatg
     2221 tttttcagag cagaatcacc ggtcactttc cacaacgcat cgttcattgt gagtgtggat
     2281 ttggccgcgt taattttacc tgtcagagat gtgtcaaacc cattgagggc gtcaagggtg
     2341 aatgcagaat aaaccggtga attctgattg tctgttttcg cttcctgacc cagagaaatg
     2401 gtggcctctt tgtctgattt gatatccgcg gtgacagctg cctgattttt cgcagtaaat
     2461 actgcgttat cctcgctcag gttataaccg ccataaccca gatagattgc agatgaccat
     2521 acatcattct ggcccggagt ccccgtcagg tttagtttac cggcaatact ggtttccttg
     2581 ctggtaaaca ggccgccctg tgcagtcatg acgccacctt tttcgatggt aaggtcagta
     2641 ccatccagtg aactggattt ggtcagggtg acagttgcct tatctcctac cttcaccgag
     2701 ccaccggttg agatgatttg gccggagaat gcggtttcac cgatggattc tcctgttgaa
     2761 atttgctggc ggaaggtaaa tccttgatta gtgatatttt taccggagtg aacatcaata
     2821 tatgcggttt tatcaccgag aatgatatct gagctggtag aaataatatc accttcgaca
     2881 gtggcattac gtcctgcaga gaatgctgcg ttatccaggt tcagtgt
//
LOCUS       DAZKHK010000286         2902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKHK010000286 DAZKHK010000000
VERSION     DAZKHK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.288"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(161..>2902)
                     /locus_tag="ACHOS5_005220"
     CDS             complement(161..>2902)
                     /locus_tag="ACHOS5_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779867.1"
                     /translation="LKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQY
                     CDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPS
                     DVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDGI
                     LRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEI
                     CDNDYAGTLTGGRILSIDAASRTLTLDREVILLEAGTSMVNLINGSGKPVRVDITAHP
                     APDRIQVSALPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEA
                     IVDNGARFEPMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRL
                     TSGNGENSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPA
                     APASIELTPGYYQITAVPHLTVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGRQWS
                     VSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGM
                     WELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAA
                     LYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQ
                     GNQLFMNDVFLKRLFAVSITSSGEPPTFSLTPDGKLTARNADISGNINANSGTLNNVV
                     IAENCTIKGTLRAENIIGDVVKTHNVSLPALSAAGDHRHATERTVTVIDDQSFDRNIV
                     IPPVFYSGIVYQGSDGDGATSICTLTVSVNDRVVFSKSSSIGTGRTSGGYPVQKDTMY
                     PGSSTPFSYFMRMASGGGDVRIKFRIEVIYYGTAPGFMTTGIQITTMKATASGFMES"
BASE COUNT          570 a          898 c          755 g          679 t
ORIGIN      
        1 atttgctgaa gatcgaaagt caggcctcgg gttaaacatg aatgtttgtc cggggccttc
       61 gtctttccgg ccttcaggtg ttccctccgg ccatcagcca aaggcacccg cgcatactgt
      121 acggtttttg tctccttccg gcaatcacga tcgtccggga ttaactttcc ataaatcctg
      181 aggctgtcgc cttcatcgtt gtgatctgta tccctgtggt cataaaaccc ggtgcagttc
      241 cataatagat gacctcaatc cggaacttga tcctgacatc tcctccccct gacgccatcc
      301 tcatgaaata gctgaacgga gtggatgacc cgggatacat ggtgtctttc tgaacggggt
      361 atccacctga cgtacgcccg gtcccgatgg atgagctttt gctgaacact acccgatcat
      421 tcacacttac cgtcagcgta cagattgaag ttgcaccatc accatcactt ccctggtaga
      481 caataccgga ataaaaaacc ggcgggataa caatgttccg gtcaaacgac tgatcatcaa
      541 tgacagtaac cgttcgttct gtcgcgtgtc tgtgatcccc tgctgcactg agagcaggaa
      601 gactgacatt gtgcgttttc accacatcgc caatgatatt ttccgctctc agagtacctt
      661 tgatggtaca gttctccgct atcacgacat tattgagcgt gcccgagttc gcattaatat
      721 tcccgctgat atccgcatta cgggctgtca gtttcccgtc cggcgtcagg gaaaacgtcg
      781 gaggctcacc tgatgaggta atactcaccg caaacagtcg tttcaggaac acatcgttca
      841 tgaacagctg attcccctgc gccacaaaca gcggcgtggt gttgccgttc tccgggttaa
      901 tcatcgcgat acggtccgcc tgcagcagta tattgctcag gggctggcca tcagcatcct
      961 caatccccgc acctataccg gcaacatacg gaatgccgtt tttcgttttc tgcaccttca
     1021 gcatgtacag cgcagccagg tcgttatttg tgtctgtctg cacccgctgt atctgctgta
     1081 tggtggcact ctggtcttcc agggttttac tgaccgtctg cgtgatttca ttgcgggttt
     1141 ctgtgatggt ggtcttcatc tccgccatct catcatcaag ctggctgtta tcaatcagct
     1201 cccacatccc ctgtgccaga tgtgtcttcc cgatttctcc tctgaaaaag tccagatacc
     1261 cttccgcatc attgctggcc cggccactgg cttccacaaa cgcagatttc cccaccaggt
     1321 tgacgctgcg cacataaaac cagaaatcct tccccggctt aatgtgcggg ccggagacac
     1381 tccactggcg gcctgtcccc agataacggg cagaggtttc cacctgagat gtgtctgcga
     1441 tttttgcctc cgaaaaccag aactcaaatt gtaccgtcgg gtcatacacc gtaagatgcg
     1501 ggaccgccgt tatctgataa taccccggcg tcagttcaat gctggccggt gctgccggtg
     1561 cgttaatccg gaacgtggtg gtggcaggtt cgccctgctg gccgtagctg tttatcgccc
     1621 tgaccgtcag ggtgtattcc cccagaggca ggccactgaa acggtgctcc gtgtcggcag
     1681 tgatggcggt ggtcaccagg cggctgtttt caccgtttcc gctggtcagg cgcagactga
     1741 agcgcacgcc cttcaccacc cgcggcgtgt cccatttcgc cagtgccaga tactggccgt
     1801 ctgaggcact cacctccacc gtaaggtgct gcaccgccgg cgggatgacg ctgttcagtg
     1861 agccggacat cggctcaaag cgggccccgt tatccacaat ggcttctttt tccggcacat
     1921 gctgcaccgc cgtgatggca aaggtaccgt ccgtgttttc ccggatggaa acacagcgga
     1981 acaggcgacg acgcagtgac ggcagggaga gtccccacac accgtatgtc tccacgccat
     2041 ccggcagggc gctgacctgt atccggtcag gggcggggtg tgcagtgatg tccacgcgca
     2101 ccggcttacc gctgccgtta atcaggttca ccatcgatgt ccctgcttcc agcagtatca
     2161 cctcacggtc cagcgtcagg gtacggctgg cggcatcgat ggacaggatg cgtccgccgg
     2221 tcagggtccc ggcatagtcg ttatcacaga tttcaatgat gtcaccgggt gtgtgacgca
     2281 gcccctgtga cccgagcgtg aaatccaccg tctgcgtttc cagcagttcg gtctttatca
     2341 cccacagtcc ggcacggtgg gcctgaccgc ggctggtaca gccgaacgca tccatcttca
     2401 gcaggttgcg cccgtagcgc aggatgccgt ccgggtcttc caccagttcc gtggaagtct
     2461 gccagccgtt ctgcgggtcg gtgtaattca cctccaccgc cgtgtgccgg tccttcaggg
     2521 cactgaagct gtagcggaac cccacgccgt tatcatccac caccacatcg ctgttggtgt
     2581 agggccacac cacatccgac gggcggtcct gaacgaacgt cagcgtctgg ccgttccata
     2641 ccggcataca gcgcatcgcc gagcagaaat cccccaggac atcccagacc ttacgctgct
     2701 gtgacaggta cgcattaaaa gtcatccgcg gctctgtgcc cccgaaacca tccgggaccg
     2761 tctggtcaca gtactgcccg atggcataca gtgcccactt gtccacatcc gcggccccca
     2821 ggcgttttcc catgccgtag cgcgggtggg tcagcatgtc ccacaggcac caggccgggt
     2881 tattgctgta tgccggtttc ag
//
LOCUS       DAZKHK010000287         2881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKHK010000287 DAZKHK010000000
VERSION     DAZKHK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.289"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            282..1007
                     /locus_tag="ACHOS5_005221"
     CDS             282..1007
                     /locus_tag="ACHOS5_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779868.1"
                     /translation="MLPGCCKNGIFISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHSERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            965..1642
                     /gene="bglJ"
                     /locus_tag="ACHOS5_005222"
     CDS             965..1642
                     /gene="bglJ"
                     /locus_tag="ACHOS5_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP9779869.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1680..2468)
                     /gene="fhuF"
                     /locus_tag="ACHOS5_005223"
     CDS             complement(1680..2468)
                     /gene="fhuF"
                     /locus_tag="ACHOS5_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP9779870.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2609..2845
                     /locus_tag="ACHOS5_005224"
     CDS             2609..2845
                     /locus_tag="ACHOS5_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP9779871.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          772 a          605 c          707 g          797 t
ORIGIN      
        1 taattataaa ttaaatgaat gagatttctg tttttcatta agtatttcta gcaacttaat
       61 gtagatattt taaatgtctc caggctattt caccaggtta tcatttgatg ctaaccttct
      121 gacacatgca gtggagttgt tgtgcagcag gagtatgctg atatgaaagt gaatgctaag
      181 gataatttat tcgcttaatc tattaatttg ctgggaatat ttaaggaatg aataccttga
      241 ataaaccatc atgattttta gacagtggat gtggaggaaa tatgttgcca ggatgctgca
      301 aaaatggaat ttttatcagt aaaatacctg ttatgcaagc agggttaaaa gaggtcatga
      361 ggactcactt ccctgaatat gaaataatat ccagcgcctc tgcggaggac cttaccttat
      421 tacaattacg tcgttccgga ttagtcattg ctgatttagc cggtgaaagt gaagatccac
      481 gttccgtttg tgagcattat tactctttaa tctcacaata tcgggaaatt cactgggttt
      541 tcatggtgtc acgctcctgg tattcccagg cagtagaatt gctcatgtgc cctacggcga
      601 cgttattgtc tgatgttgaa cccattgaga atctggtcaa gaccgtacgt tccggcaata
      661 cgcactcaga gcgtatcagc gccatgctga cctctccggc gatgactgaa actcatgatt
      721 ttagctatcg ctccgtcatt ctcactcttt cagagcgcaa ggtactgcgg ctattaggta
      781 aaggatgggg catcaaccag atagcttcat tgcttaagaa aagtaataaa actattagcg
      841 cccaaaaaaa cagtgcgatg cgtcgactgg caattcacag caacgctgaa atgtatgcat
      901 ggataaatag cgcgcagggt gcaagagaac ttaacttgcc ttctgtttat ggagatgccg
      961 cagaatggaa cacagccgaa ttaagaagag aaatgtcgca ctcatagaaa aatgcgtcat
     1021 gagtagtatc ggtattgaga gtttattcag aaagtttgca ggtaaccctt ataagctcca
     1081 tacctatacc agtcaggagt catttcagga tgccatgtcg cggatctcgt ttgcagcggt
     1141 cattttttct ttttctgcca tgagaagtgc gcgcagagag ggattatcct gcctgactga
     1201 actggcgatt aagtttccgc gtacccggcg tttagttatt gcggatgatg atattgaagc
     1261 tcggctgatt ggttcattat cgccatcacc gctggacggt gtattaagta aagcgtcaac
     1321 gctggagatt tttcatcagg aactcttttt gtcattaaat ggtgtacgtc aggcgaccga
     1381 tcgactgaac aatcagtggt acattaacca aagccggacg ttaagcccga cggagagaga
     1441 aatattgcgc tttatgtcgc gtggctactc aatgacacaa attgccgagc agcttaaacg
     1501 caatatcaaa acgatccgtg cacataaatt taatgtgatg tcgaaactgg gcgtcagttc
     1561 tgatgcaggg ttgttggagg ccgcagatat tctgttatgt atgcggcatt gcgaaacaag
     1621 taatgtgttg catccttatt aatccgcatg atgccgggtt tacttccccg gcagtgcttt
     1681 catttcagcg tacaatcgcc acattgctgc acatccggta agcgataacg ctggcagcaa
     1741 gtgcggcgca ccagcaggcc gtcgcgtagc accacggtac gccagagtgg attatcttca
     1801 ccggtagtaa gcgttttctc aaaaaagagg gcatagcgca gcgattcaac cgtcgcctcg
     1861 ccgagcagct gtttcatctc agtcagatac cagttgatca aataaccggt attgctccag
     1921 ataagtttgc cgttaatctc tcccgttgct tctagtgcct gcacaaccgg aaccagcgcc
     1981 tggctgatta acgtttccat tcgctgctgc ggcgaatgta gtgttgcgtt tttatcttca
     2041 cacacatcga cccagaaaca ggcggcgcgt ccggtttcgt gaaactcaac gtggacatgt
     2101 tccggcgaca catctaatgc tttttcctga gtcagcagcg ccagcattaa tggtggtacc
     2161 atcaggccga tataccattg cgcccacagt gagatcagcg gtttgttttc gcggatcatc
     2221 aaaggttggt tgcggtagat atgatcggaa tagaccgcga gtagagaact tagcgcatta
     2281 ggtgatgacc attgcgccag cgtcatggcg ttaagtgggg cgggttcatc caggcggata
     2341 aactccagca aatgttcacg atgttcggcg atcgtcgccc gcacggcttg tgcaagccca
     2401 gcatcctgcg gctggagatg cgttcgccag ataacatctt catagagcgg tgcggaacga
     2461 taggccataa tcgtgatagt aatctaaatg ataattattg ctaatcctat cgataggttt
     2521 acccgatagc aagggatttc tctggcttgc aaatgataaa aattatcata tgatattagt
     2581 tatcattatc aatgaaagag atgaaaccat gttgcaacgt acgctgggca gtggctgggg
     2641 agtgttgctg ccgggattgc tgattgcagg gctgatgtat gcggatttat cgccggatca
     2701 atggcggatt gtcattctga tgggattggt attgacgccg gtgatgttgt atcacaaaca
     2761 gttacggcat tacgttttgc taccatcgtg cctggcgctt attgctggca tcatgctgat
     2821 gataatgaat ttgaatcagg ggtgataaaa cagtgaagag acaagaaaag aagtaaagat
     2881 a
//
LOCUS       DAZKHK010000288         2856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKHK010000288 DAZKHK010000000
VERSION     DAZKHK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.290"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..670
                     /locus_tag="ACHOS5_005225"
     CDS             <1..670
                     /locus_tag="ACHOS5_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP9779872.1"
                     /translation="FRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNL
                     GTIDEPGHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGD
                     AIQATKAAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPF
                     EGLKERPFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIG
                     TFTA"
     gene            798..1334
                     /gene="yfdR"
                     /locus_tag="ACHOS5_005226"
     CDS             798..1334
                     /gene="yfdR"
                     /locus_tag="ACHOS5_005226"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9779873.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIIINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPSHA
                     YGMFMERFNELSELRKCA"
     gene            1325..1666
                     /locus_tag="ACHOS5_005227"
     CDS             1325..1666
                     /locus_tag="ACHOS5_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779874.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFTEAEAKISA
                     LAAENELARKAVQAFCDVVGDNTEVIAEEVGRDGVLVILEAMKATGNMPATDAFLAEV
                     RAQGGIWLVTR"
     gene            1648..1779
                     /locus_tag="ACHOS5_005228"
     CDS             1648..1779
                     /locus_tag="ACHOS5_005228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779875.1"
                     /translation="MARNAMIDFVDGEVGPNKNVPGLIRGTEICVSIAEQLRKGGNQ"
     gene            1776..2120
                     /locus_tag="ACHOS5_005229"
     CDS             1776..2120
                     /locus_tag="ACHOS5_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9779876.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRTVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTSAGIRIEGE"
     gene            2122..2474
                     /locus_tag="ACHOS5_005230"
                     /pseudo
     CDS             2122..2474
                     /locus_tag="ACHOS5_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2471..>2856
                     /locus_tag="ACHOS5_005231"
     CDS             2471..>2856
                     /locus_tag="ACHOS5_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779877.1"
                     /translation="MIDRTKLEHILEYARQQKCIGQLCKIPPGDMLEIVEMAMRKAGN
                     SPVTPARLPGGFTIEDAKELYEDLVRSHISKALSGEKMKKKERDADLRWIHGVIVQAA
                     WFVKASLEQNALTGNSQVIPDDGREQ"
BASE COUNT          760 a          618 c          768 g          710 t
ORIGIN      
        1 cttccgtttc cgtggtgcgc tttccactgc cagcatcgat gactttaccc gttattctaa
       61 agatcttgca gatgaaggca cccgctgctt tatcgatgct gataatatgc gtgccgtcag
      121 tgtgcttaac ctgggtacta ttgatgaacc aggtcacgca gataacaccg ccactctcaa
      181 actgaaaaag acagcaccgt tctctgctct gttgtctgtt aatggcgagc gtaactccca
      241 gaagtcactg gcagaatgga ttgaagactg ggccgactac cttgtgggct ttgatgctaa
      301 tggtgacgcc attcaggcaa caaaagcggc tgcggcagtc cgtaaaatca caattgaagc
      361 gaaccagact gctgattttg aagacaatga cttcagcggc aaacgctccc tgatggagtc
      421 tgtcgaagcg aagaccaaag acattatgcc agtggcattt gaatttaaat gcgttccgtt
      481 tgaaggcctg aaagaacgtc catttaaatt acgcctcagc attatcactg gcgatcgtcc
      541 tgtactggtt ctgcgcatta ttcagctgga agcggtgcag gaagatatgg ctaacgaatt
      601 tcgtgatctg cttgttgaga aattcaaaga cagcaaagta gaaaccttta ttggtacttt
      661 caccgcctga tttcattact gcaaatgccc ctgcgggggc atttatggaa acgtaattaa
      721 ctcaataatc gccggatggt gaggacttcc ttttaccaga attcagcgcg gtgcagcgca
      781 tatacgtgga gaacaaaatg tcatttatta aaactttttc cgggaagcat ttttattatg
      841 acaagataaa taaagacgac atcattatta acgatatcgc ggtttccctt tcaaatatct
      901 gtcgctttgc aggacatctt tcacacttct acagcgtcgc ccaacatgcg gtgctttgca
      961 gccagctggt accgcaggaa tttgcttttg aagcgttaat gcatgatgca acagaagctt
     1021 attgtcaaga catccccgcg ccactgaaac gccttcttcc tgactataaa cggatggaag
     1081 aaaaaataga cgccgtaatc cgtgagaaat acgggttacc cccggttatg agcacgcctg
     1141 tgaaatatgc cgatctcatc atgctggcaa ccgaacgccg cgatctcggg cttgatgatg
     1201 gctctttctg gcctgtactg gaaggtatcc cggcgacaga gatgttcaaa gttattccac
     1261 tgtcaccaag ccatgcctac gggatgttta tggaacgttt taacgagtta tcggagttac
     1321 gcaaatgcgc atgaatgttt tcgaaatgga agggtttctt cgcgggaaat gtgtaccgcg
     1381 agatctgaaa gtgaacgaaa caaatgctga gtacctggta cgtaaattta ctgaagcgga
     1441 ggctaagatt tcggcgcttg cggcggagaa tgagctggct cgtaaggcag ttcaggcatt
     1501 ctgcgatgtt gttggcgaca acaccgaggt tatcgctgag gaggttgggc gagatggcgt
     1561 tctggttatt ttggaggcca tgaaggcaac aggaaatatg ccagccaccg atgcttttct
     1621 ggctgaagtg cgggcacagg gggggatatg gctcgtaacg cgatgattga ttttgttgat
     1681 ggtgaagttg ggccaaacaa gaacgttccg gggctgatta gaggcacaga gatatgcgta
     1741 agtattgctg aacagcttcg taaaggaggc aaccagtgag tgggattaat tatcaggcgc
     1801 tgcgtgaggc ggcacaaaac tatcagtcga tgctggcgtg gtatcaggag aaaccagaca
     1861 gtccaaacgc tgagcaggat tgtgatgcag ctttggctgc gtttaagcgt gaaatccgtc
     1921 atcgtgaagt ggatattatc gctgatttgc tggatgaact ggaggaagca aaacagcgta
     1981 tcgacgagca ggagtcccgc acagtgaagt taccagaacc attcaagctg gctaaatcat
     2041 caagtggatt aacgtactac tacgctgatg aggtcaacgc cgcactgact tctgctggta
     2101 ttcgcataga aggagagtga gatgaccact attaccaaag agcgactgca atggctggct
     2161 aacatttctg gccgcgatga cattgacgat atagacgtcg gtgaaattcg tgagctggcg
     2221 cttatcgcac tggcatcgct ggaagcagag cctgtggcgt catgcattat tgaagatggg
     2281 gcatgtgtgt tgacgggttc ggtgagtatg ttggtcactc gctgcctgat ggaacgcacc
     2341 agctatacgc cgccccgcca gtgcctgata agttgccgcg tgaatacata agaggttggc
     2401 ctcttgcgca tagtgattat gcagaaggat ggaacgattg ccgtgctgca atgcttaatg
     2461 gagataaatc atgattgatc gaaccaaact ggagcacatt ctcgaatatg ccaggcagca
     2521 gaaatgtatt gggcaacttt gtaaaattcc accaggagat atgcttgaaa tcgtggaaat
     2581 ggccatgcgt aaggctggca actctccggt aactccggct cgcctgcctg gtggtttcac
     2641 cattgaggat gcgaaggagt tatatgaaga cctggtacgc agccacataa gcaaggcttt
     2701 aagtggcgaa aagatgaaga agaaggagcg cgatgctgat ttgcgctgga tccatggggt
     2761 tatagtccag gcagcctggt ttgtaaaagc atcactggag cagaatgcgc taacgggtaa
     2821 ctctcaggta atccctgatg acggtcgcga acagtt
//
LOCUS       DAZKHK010000289         2779 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKHK010000289 DAZKHK010000000
VERSION     DAZKHK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.291"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            257..940
                     /locus_tag="ACHOS5_005232"
     CDS             257..940
                     /locus_tag="ACHOS5_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9779878.1"
                     /translation="MNLKKTLLSVLMILQLCLLVGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGKILSIDAVENGSVIRISLVNLLSVPVSNMGFYATWGGEKPTDINAL
                     AKWQQLLFSTSMNSSLKLLPGQWQDINLTLKGVSPNNLKYLKLAINMTNIQFDRLQPA
                     ESPQRKNKK"
     gene            984..>2779
                     /locus_tag="ACHOS5_005233"
     CDS             984..>2779
                     /locus_tag="ACHOS5_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP9779879.1"
                     /translation="MNKIFKVIWNPATGSYNVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGAYAEVSLDGGTSAETTPLTNGWIAIGHDSIASSVVQSGAAAAIGYQARALGE
                     GSTALGYQSLATGDRAMALGQMSKSSGNRAAALGSGANAAGDHSLALGGATNAQGEYS
                     VAIGRAATTDSNYALSMGYMAKANGLYSLAMGAGSATSNDNAIAIGNKTQALGVNSTA
                     LGNASQASGESSIALGSTSEASEQNAIALGQGSIASKVNSIALGSNSLSSGENAIALG
                     EGSAAGGSNSLAFGSQSRANGNDSVALGVGAAAATDNSVAIGAGSTTDASNTVSVGNS
                     ATKRKIVNMAAGAISNTSTDAINGSQLYTISDSVAKRLGGGATVGSDGTVTAVSYALR
                     SGTYNNVGDALSGIDNNTLQWNKTAGAFSANHGANATNKITNVAKGTVSATSTDVVNG
                     SQLYDLQQDALLWNGTAFSAAHGTEATSKITNVTAGNLTAGSTDAVNGSQLKTTNDNV
                     TTNTTNIATNTTNITNLTDAVNGLGDDSLLWNKAAGAFSAAHGTEATSKITNVTAGNL
                     TAGSTDAVNGSQLKTTNDNVTTNTTNIATNTT"
BASE COUNT          853 a          669 c          652 g          605 t
ORIGIN      
        1 tctttccctg ccctgtgcct ttatccggtg aaacatttga catcgattga caccgatcat
       61 taaaatccgc gcgattaata ttattggcta caaactgaaa tcgtttgaca tttaataaca
      121 tattcgcaca accaattaat acaaaaactc tctaacaaat ttattttgcg gatatgcccc
      181 attccaatca ttcataaaaa aaatgattgt gccgtaatta tcaactgtat cgggattatg
      241 aaaaggaatt catcctatga acctaaagaa aacactgtta agcgtgttaa tgatattgca
      301 actttgctta ttggtagggt gtgactatat tgaaaaagcg agtaaggtcg acgatctcgt
      361 tacacagcaa gagttgcaaa aaagcaaaat tgaggcgctt gaaaaacaac aagaactcga
      421 caagcgcaag atagaacact ttgaaaaaca acaaactacc atcataaaca gtaccaaaac
      481 gctcgctggc gtggtgaagg cagttaaaga caaacaggac gaatttgtct ttacagaatt
      541 taacccggca caaacccaat acttcatttt aaataacggt tctgttggtt tggcagggaa
      601 aatactgtct attgacgcag tagaaaacgg cagcgttatt cgtatttcac tggttaactt
      661 attaagtgtt cctgtatcaa atatgggttt ctacgcgaca tgggggggag aaaaacccac
      721 cgacatcaac gcattagcaa aatggcagca attgctattt agtacctcaa tgaactcctc
      781 cctgaaatta ttaccaggtc aatggcaaga cattaatttg acgctaaaag gtgtctcgcc
      841 caacaacctc aaatatctga aattagccat caacatgaca aatattcagt tcgaccgtct
      901 tcaacctgct gaatctccac agcggaaaaa caaaaaataa atgcagcttc tgcgcttata
      961 tcaaggaata gagagcatca ataatgaata aaatatttaa agttatctgg aatccggcaa
     1021 caggcagtta caacgttgcc agcgaaacgg cgaagagccg tggtaaaaaa agcgggcgca
     1081 gtaagctgtt aatttctgca ctggttgcgg gtgggttgtt gtcgtcattt ggagcatatg
     1141 ccgaagtatc acttgatggt ggtacaagtg ctgaaacaac cccactaacc aatggttgga
     1201 ttgctattgg ccatgattcg attgcatcgt cagtagtgca atcaggagct gctgccgcaa
     1261 tagggtatca ggcaagagct ttaggtgaag gaagtactgc attaggttac caaagcctgg
     1321 caacaggaga tcgtgcaatg gctttgggac aaatgtcaaa atcttcagga aaccgagccg
     1381 cagcgctcgg gagtggtgca aatgctgctg gtgatcattc cctggcatta gggggggcga
     1441 ctaatgctca aggtgagtat tctgtggcaa tagggcgggc tgctactaca gatagcaact
     1501 acgcgctttc tatggggtat atggcgaaag ctaatggact atacagcctg gcaatgggtg
     1561 caggaagtgc tacttcgaac gataacgcca tcgcgattgg gaacaaaacg caagccctgg
     1621 gagtgaattc tacagccctg ggtaatgcaa gtcaggcatc tggcgaatcc agtattgcat
     1681 taggtagcac cagtgaggcc agcgaacaaa atgcgattgc gctggggcaa ggtagcattg
     1741 caagcaaagt gaactcaatc gcgttgggaa gtaacagttt gtcctcggga gagaatgcca
     1801 tcgcattggg agagggtagt gccgctggtg gcagcaacag ccttgctttc ggtagccagt
     1861 ccagggcaaa cggcaatgat tctgtcgccc tcggtgtagg ggctgcagca gcgaccgaca
     1921 attctgtcgc tatcggcgca ggatcgacca cagatgcaag caatacggtt tcagttggca
     1981 acagcgcaac aaaacgcaaa attgttaata tggcagctgg tgccataagc aacaccagta
     2041 ccgatgccat caacggctca cagctttata cgatcagtga ttcagtcgcc aagagactcg
     2101 gaggaggcgc tactgtaggc agcgatggca ccgtaaccgc agtaagctac gcgttgagaa
     2161 gcggaaccta taataacgtg ggtgatgctc tgtcaggaat cgacaataat accctacaat
     2221 ggaataaaac cgcgggggcg ttcagcgcca atcacggtgc aaatgccacc aacaaaatca
     2281 ctaatgttgc taaaggtacg gtttctgcaa ccagcaccga tgtagtaaac ggctctcaat
     2341 tgtacgacct gcagcaggat gctctgttgt ggaacggcac agcattcagt gccgcacacg
     2401 gcaccgaagc caccagcaaa atcactaacg tcaccgctgg caacctgact gccggcagca
     2461 ctgacgccgt taacggctct cagctcaaaa ccaccaacga caacgtgacg accaacacca
     2521 ccaacatcgc cactaacacc accaatatca ccaacctgac tgacgctgtt aacggtctcg
     2581 gtgacgactc cctgctgtgg aacaaagcgg ctggcgcatt cagcgccgcg cacggcaccg
     2641 aagccaccag caaaatcacc aacgtcaccg ctggcaacct gactgccggc agcactgacg
     2701 ccgttaacgg ctcccagctc aaaaccacca acgacaacgt gacgaccaac accaccaaca
     2761 tcgccactaa caccaccaa
//
LOCUS       DAZKHK010000290         2748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKHK010000290 DAZKHK010000000
VERSION     DAZKHK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.292"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(707..835)
                     /locus_tag="ACHOS5_005234"
                     /pseudo
     CDS             complement(707..835)
                     /locus_tag="ACHOS5_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(811..960)
                     /locus_tag="ACHOS5_005235"
                     /pseudo
     CDS             complement(811..960)
                     /locus_tag="ACHOS5_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1045..1242)
                     /locus_tag="ACHOS5_005236"
     CDS             complement(1045..1242)
                     /locus_tag="ACHOS5_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9779880.1"
                     /translation="MESLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(1262..1750)
                     /locus_tag="ACHOS5_005237"
     CDS             complement(1262..1750)
                     /locus_tag="ACHOS5_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9779881.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLAELINKVSENGYS
                     LRVVDKSDQQSTSTPPPLTTLTGIRCSTAHITETDNARLYSLSHQTNDDGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(1747..2124)
                     /locus_tag="ACHOS5_005238"
     CDS             complement(1747..2124)
                     /locus_tag="ACHOS5_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9779882.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKR"
     gene            complement(2215..2583)
                     /locus_tag="ACHOS5_005239"
     CDS             complement(2215..2583)
                     /locus_tag="ACHOS5_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9779883.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYSTPETKK"
BASE COUNT          694 a          659 c          702 g          693 t
ORIGIN      
        1 aatggcatgg tagcaaagtt ttcatcacat acctcctttg caccagaata aacgccagag
       61 acctgaaatt gcgagtacac tacagaaatt gtactgccgg tcccatcagc ataaaaccat
      121 agttacacag gtaatcatca ttattcacca agataataaa tattattcgg tagaatagcg
      181 ttctcaggtg gtcagtaaaa ttgtcttcaa caagatgtct gcgcatctcg tctgctagcc
      241 ctacataact gctctcgacc gaaactattt attgaatgtt caccaaggtg ggcgtggaga
      301 caaaatgacg tcttttgata ttagctacct gagccacatg gtcaaaacgt tcaagtagca
      361 tagcgaaacc accctgccca cgtaatgtgg acgatgcagc ttccagccag agcaaacttg
      421 ggaccatcga ctacaaaatg aagaatactg aactgctttt ctggcaggcc tatatactgc
      481 acggatatgg gtgatttcag acacaaaaaa agccgctctt gagcgactcg atttgcatac
      541 ggtgtggtgc gaaggccgga ctcaaacatg tgacttaacc tcatgattta aaattgttaa
      601 taataaacaa tatcatctta taccaacaca gataccaaca cgaaaaatca ctggttttcg
      661 gcatcgaacc aggacaatca gctcctgatt gagataccga gctctgttac tcgtcatctt
      721 cctcgacagg cttcattgca tcaagtagta ctcgcaccat cgcgatagaa ccaatccgga
      781 taaaagcatc ttctccatct atatctgtta actgatatcg ctcacttgat gccggataat
      841 tgtgtcgatt tgcagcagtg aactctttcg tacagccaga atgtggcgga tgctggttga
      901 gcagataacg tccgtattcc cgttgaccac ttccggacgg taggctgtga tatcagacat
      961 aatcgatatt cctttttgca gataaatgaa aatgccagcc ctgttcaggc tggcgtcagt
     1021 ggaatgaagt ctggtgagta agactcaccg gttctggtgc agaagctgca actggcgcag
     1081 tgtacggata tcttccctgg cctgttcaat acagggcagc agtgctgccg aatccgtttt
     1141 atcctcatcg ttgtcaaaga taattcccga ttcgcagtcg atattgtcct gcagccacgc
     1201 aatcagaata tccagtgctg tttctgtggt taatgattcc atactgtgtc ttttgtcctg
     1261 gttaccagtt gaaagtgggc aatcccggca ggcattcagc gctggcatcc agatgaatga
     1321 gactgacgcc ataacgccgg attagtgtga caaccagacg acggaacgtt ctggacaggc
     1381 ccaggcgttt aagccgcaga accggatacg accacgcatc ggtacgtaac agatagccgc
     1441 tgccggtaaa atgaatccat tctgattcgc cgtcgtcatt agtctggtgt gacagcgagt
     1501 acagcctggc gttgtctgtt tccgtgatat gtgcggtgct gcagcgtatg ccggtaaggg
     1561 ttgttaacgg tggtggtgta ctggttgact gctggtcgga tttatcgacc acgcggagtg
     1621 aataaccgtt ttcagagacc ttgttaatca gttcagcgag attaacacca tcgacgtcaa
     1681 cgacaatgcg ccccatgttc agcgcctgta cgttaacgct gtcggcttcc agcgtcaggg
     1741 ccagtttcat cgtttcgcct ccggatgctt ccctagggta atgttattta ccgttctgta
     1801 attgtcgcga gtcatcaggc ctgttgcccg gcgagcccgg aggatatcga tgctgtttat
     1861 taactgagag tgggtacagg cgctgaatcc cgactggtcg gtacgtacca gcgcgtattt
     1921 ttcaacgaga aagttcaccg cgtcacacag tgaaatgcct gcctcaatat gctgctcaat
     1981 cacacgttca tcggcaaacg gtgtgtcatt cagtgtaagg ccgtaatgct ggtccagcag
     2041 tcgggagagc agagtctgcc agatggtgac gggagacggg cagcgcgatg cctcccgtac
     2101 gtgtgtatcg ggtaatgttt tcatactgaa gattttcctg atatgcagag ataaaaaacg
     2161 ggaaagtggc gtggtgaaaa acaccaggcc ggagcagagg gttattccgg tggtttactt
     2221 tttcgtttcg ggcgtcgaat aaacagccag ataaacgtaa ccaccgctac cgagggtgtc
     2281 agctttgcag gtcagacctt tcgcatacag cgtgacggta tgctgatggc ggggattcag
     2341 ttcaccgctg gtgagcatga gttccagttg tttcatcagc agcggaaagg cctggtccag
     2401 atggtatgca tctgcgtcgc tgaagcggcc tctgataccg gcgcggtcgg caaggtaatg
     2461 caaccggtta ccctcctgca ccagacgggc accaaaacag ggcctcactg tgcagggaag
     2521 cccccaccag gggcggtcgt gattgtcgtc gggatgagtt atcccggaga gtttgtctga
     2581 cacgataaaa tcctctctga aaagtggtta agaatggttt aacgatgaca ggtcagccgg
     2641 cgcgctgacc tgtttcggtg aagatgtatg cattgatgag aatggcctca tccacagtgg
     2701 cgtctgagtc catctggtcg tactcgcggg tgacggacat cagttcag
//
LOCUS       DAZKHK010000291         2726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKHK010000291 DAZKHK010000000
VERSION     DAZKHK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.293"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>2726
                     /locus_tag="ACHOS5_005240"
     CDS             <1..>2726
                     /locus_tag="ACHOS5_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779884.1"
                     /translation="PEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETDKGVAFY
                     SRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVVRGVMSGIKDSDLKVRVVKSQKEA
                     EALAGELFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPA
                     EYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAA
                     WERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGVGASSQYS
                     RSGKPDPFKADAYDAKQFASEVRRIAGLQSVSRREMLRVGDTPRVLRALGAPDLELVM
                     PASVVHKATRPEIREHFVSVETLADLPQLLTDPVVVMRSKTESDALVSLIQATDTKGN
                     PVIVAIHTHVKGDGFAEINKIASAYGKDKRSALQDQLDTSLLYLNKEKAAEWLHAVGL
                     QLPEANTINGSSGFKILHPEDIRKGPYYSRTSSLTPEDTIASRFVRQMQDKFQVLKAV
                     QENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLD
                     EYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVD
                     DMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRE
                     SRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDR
                     PDTMRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYYIKLHDPRLMRA
                     MKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSD
                     GKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGTQWQKVWKEFVEDGG"
BASE COUNT          711 a          641 c          807 g          567 t
ORIGIN      
        1 cctgagcaca acacagataa cacctgggct tatccgacga atgcggaact ggatggcggt
       61 attcgtgatg cattcgatca cctgttcagt acgctgaaaa cccgtgagac ggacaaggga
      121 gttgcgtttt attcccgtaa gggcgtgacc cgcacacctg aaggcaatct catttcggat
      181 gttaaccgca gtgcggaagc caaaggcagc ccggtcccgc aggttgaagc ggttgtccgt
      241 ggcgtgatga gcggcattaa ggacagtgat ctgaaggtcc gtgtggtgaa gtcacagaaa
      301 gaggctgaag cgctggcggg cgaattgttc gatggttacg gcagggtgca cgcattctat
      361 cgtccggata aacgagaaat tgtcctggtg gcggataaca tccctgacgg gcggaccgta
      421 cgcgagaagt tgcgtcacga gattatccac catgccatgg agcatgttgt cacgccagcg
      481 gaatatcaga cgattatcaa aaccgtgctg aaaacccgcg acagtgataa cgccaccatc
      541 cgtgaagcct ggcgtaaggt tgatgcgtcc tatggtaagg aatcaccgga agtgcaggcg
      601 ggtgaatttc tggcgcatat ggcagagaaa cagccgaata aatttgtggc ggcatgggag
      661 cgtgttgttg ccctggtcaa aggggtactg cgtcgtacgg ggttactgaa accgacggaa
      721 ctgaacgata tcagacttgt tcgcgagacc atccgtacgt taggccagcg tgtgcgggaa
      781 ggttacacac cgcgtgagga tggagtgggt gcatcgtctc agtactcccg tagtggtaaa
      841 cctgatccgt ttaaggctga cgcctatgat gcaaagcagt ttgcttcaga agtgcgtcgc
      901 attgccggat tacaatcggt ttccagaagg gaaatgctca gagttgggga tacgcctcgt
      961 gttttgcgcg cattgggggc accagatctt gaactggtta tgcctgcatc tgttgtgcat
     1021 aaggctacga gaccagagat cagagaacac tttgtgtcag tggaaacact ggctgatttg
     1081 cctcaacttc tgacagatcc ggtagtagtt atgcgttcga aaactgaatc ggatgcgttg
     1141 gtatctttaa ttcaggccac cgatacaaaa gggaatcctg tcattgttgc aattcatacc
     1201 catgtgaaag gcgatggatt tgcagagata aacaaaattg ccagtgccta tggaaaggac
     1261 aaaaggagtg ctttgcagga tcagcttgat acatctttgc tgtatcttaa taaagaaaaa
     1321 gccgctgaat ggcttcatgc agtcgggctc caattgcccg aggcgaacac catcaacggc
     1381 tcctctggat ttaaaatact acatcctgag gatattcgca aggggccgta ttactcccgt
     1441 accagcagtc tgacgccgga ggatacaatt gcatcgcgtt ttgtgcgcca gatgcaggat
     1501 aaattccagg tgctgaaagc tgttcaggag aatatccgta aaactggcgg aaaaatagac
     1561 gacagtaaca acgcttatat ggcggaagaa ctcttccacg ggaaggcgga aaacgacctg
     1621 aacgtgatga aggagcgcta tgttcagccg ctggctaaat tactggcgga ctataagatt
     1681 gcgcaggccg atctggatga gtacctctac gcccgtcacg cgccggaacg taacgcgcat
     1741 atcgcgaaaa tcaacccgaa aatgccggac ggcggttcgg ggatgaccaa cgcggaggcg
     1801 gcggaaatca tgcagcgtgt gcgtaacagc ggcaaacagg cacagtatga ccgtctggca
     1861 gggattgttg acgatatgct ggcccgtcgc cgtgagctta tccgtgaggc cggactggaa
     1921 gagagcggtg tggtggatgc ctggcagaac gcctaccgtt actacgttcc cctgaaaggc
     1981 caggatgttg acggtgtggt gtcactgccc cgtacaggta agggcttcac catcggcgga
     2041 cgtgaaagca ggcaggccat ggggcgtgca tcccgtgcac agtctccgtc cactcaggcg
     2101 atacaggacc tgagcgaatc gctgatccgc catcgcaaaa acgaagtggg taacgccttc
     2161 ctgaaactgg tgcaggataa tcccgacaag gattactggc aggtattcac tgatgacaga
     2221 ccggatacca tgcgtgtgat tgcagagcgc aaggaccagg aaactggtga aaccattcgc
     2281 gaagttgtcg agcgtccggt ggcgatggca atgatggcag accggtactt caccaccaaa
     2341 aagaacggca aaacgtacta catcaaactc catgacccgc gcctgatgcg tgcgatgaag
     2401 aatatgggac cggaaaccag taacgccgta atccgtacgc tggggaaagt taaccgcttc
     2461 ctggcaacgg tgaacacgtc gtataacccg gaattcctgg tcagtaactt catccgtgac
     2521 gtgcagacgg cggtgatgaa cctgaaggca gagcagggaa ggagcgacgg taaactgaaa
     2581 gggctggata acttatctgc cctggctgta gtcaaagaca gccgttctgc aatgtcagcc
     2641 gtgtacgcca gtctgcgagg caaaactctc acgggcaaag gtacacagtg gcagaaggtg
     2701 tggaaagagt ttgttgagga cggagg
//
LOCUS       DAZKHK010000292         2676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKHK010000292 DAZKHK010000000
VERSION     DAZKHK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.294"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHOS5_005241"
     CDS             <1..315
                     /locus_tag="ACHOS5_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779885.1"
                     /translation="SGDDWNITIREDGKVTPPSHKHSEELIEFAIDYLKNNKKQGLMK
                     RIGRCMGYLQVAAEIEALASGADRDAEVRKALLRDFNTPPFKKEPDSWVQPGLTYLKG
                     RI"
     gene            complement(327..641)
                     /locus_tag="ACHOS5_005242"
     CDS             complement(327..641)
                     /locus_tag="ACHOS5_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779886.1"
                     /translation="MHNHQHNYDLCLQAINERVKSECLLLLPQEHKAVKVIQAEPYGA
                     LTTATLSIISGALTPTLLLRLKDNIDDWLNEELSYLDCEWDNHYAKSQKDRLFRQLSG
                     NR"
     gene            770..1303
                     /locus_tag="ACHOS5_005243"
     CDS             770..1303
                     /locus_tag="ACHOS5_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9779887.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            1577..2272
                     /locus_tag="ACHOS5_005244"
     CDS             1577..2272
                     /locus_tag="ACHOS5_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9779888.1"
                     /translation="MKEMISVDREISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPQTLPEALRFAAKLAEQKAELENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            2367..2498
                     /locus_tag="ACHOS5_005245"
     CDS             2367..2498
                     /locus_tag="ACHOS5_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779889.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            2510..>2676
                     /locus_tag="ACHOS5_005246"
     CDS             2510..>2676
                     /locus_tag="ACHOS5_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9779890.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRDSAITYKAQRDKKARELELA
                     NATITDMQVRQR"
BASE COUNT          705 a          560 c          729 g          682 t
ORIGIN      
        1 tccggtgatg actggaatat cacgatccgc gaagatggaa aagtaacgcc gccatcgcat
       61 aagcacagtg aggagcttat tgaatttgcc attgattacc tgaagaacaa taaaaagcag
      121 gggctgatga agcgcattgg tcgttgcatg ggatatctgc aggtggctgc tgagattgaa
      181 gcgctggcca gtggtgctga cagggatgct gaggtgagga aggcccttct tcgggatttc
      241 aatactccac cctttaagaa agagcctgat agctgggtcc agccgggtct gacttatctc
      301 aaagggcgga tataagttca ggggcgttat ctgttacctg ataattgtct gaacaggcga
      361 tctttttgtg atttggcgta atgattatcc cattcgcaat caaggtaact taattcttca
      421 tttaaccagt catctatatt gtctttcagg cgaaggagca aggttggcgt taatgctccg
      481 gatataatac tgagagttgc agtagtgaga gctccatacg gttcagcctg aatgaccttt
      541 actgctttgt gttcttgcgg gaggagtaag aggcattccg attttacccg ctcatttatg
      601 gcttgcaggc ataaatcata attatgttga tggttatgca tggttaatcc tcgactggaa
      661 ttgtcagata tattttcagc cagcagataa aacgccagtg cccaccactg gcgggctgaa
      721 gacttaacat atccagggat tcggaaccga taaatcctga taaatatcca tgaacgcaaa
      781 aatcaaatac ggcctgtcag ccgccgttct ggcgctgatt gccgcagggg cttctgcgcc
      841 tgaaatcctc gaccagtttc tggatgaaaa ggaaggcaac cacaccacgg cataccgtga
      901 tggtgcgggt atctggacca tctgccgtgg agccacccgg gtggatggta agcctgtgat
      961 tcctggcatg aagctgtcga aggaaaaatg cgaccaggtt aacgccattg aacgggataa
     1021 ggcgctggca tgggtggaga aaaacatcag agtgccactg actgaacccc agaaagcggg
     1081 tattgcgtca ttctgtcctt acaacattgg cccaggtaag tgtttcccgt cgacgtttta
     1141 taaacgaatt aatgcaggcg atcgcagggg agcgtgtgag gcgattcgct ggtggattaa
     1201 ggacggtggc agagactgcc gtattcgctc aaataattgc tacggtcaga tatcccgtcg
     1261 tgaccaggag agtgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga
     1321 aaaatgacgt tggtcaacgc gggtggataa cacgaaatcc tgcgaactgg caaaacctaa
     1381 gtgaataaag tcaggaacat tgtttcacgc agaggcaccg taatggtgcc tttgtcattt
     1441 ctgcgcttcg cacaagcgta aataaaccaa agaacctttc aggatgagcc ctggtggata
     1501 accggcagtg gtctggttaa ccctctttgg gctggttatt cctgtgcgca gggttcatca
     1561 ctaaaaggaa tcaaccatga aagagatgat ttctgtcgat cgtgaaatat ccatgagtag
     1621 tctggatttt ctgaataaca ttattaatcc agcccgggca gaagccggag aagtccctca
     1681 tgaaccgcgt aagtttcttg caaaaattga ggatgagcta gagcttgatg gaaccggaaa
     1741 aaaattccgg ttaaacaata accagacaag aacggcatac tatgatctgg attttgacca
     1801 gatgatgctc gttggcatga gagagtcaaa ggccgttcgt cgttctgtgc tggcaaggct
     1861 caaagcgatg catggtattc agatccccca gactttacct gaggcgttgc gatttgcggc
     1921 aaaactggca gaacagaaag cagagctgga aaatcaactg gcaatagcag caccgaaggc
     1981 tgaatttgtt gataactatg ttgaagcatc tggtctgatg ggattccggg aagttgctaa
     2041 gttactcggt atcaaagaaa ccgatttccg gctgtttttg ttggagaacg gaataatgta
     2101 tcgcctcgct ggaaaaatga cgccttactc gcatcacctg gatgcggggc ggtttagcgt
     2161 gaaaacgggc gaggcgggca atggtcatgc ttttacgcag gttaaattca ccccaaaagg
     2221 tgttcagtgg attgctggtc tgcttgctgc atggagagct accgcagcat gaagatgata
     2281 aaaatggact ggaaattttt gctggtctgg ctgattccgt ttttatgggt ggttgcctgg
     2341 ttaattactg ctattaagag gtaaagatgt cagacaaact cataatgctg gcgaagggcc
     2401 tttgtgtaat cgtcggtatt tcattttcac taatgctggt tgctcttttt ctttccataa
     2461 cctggatggc gttgacttcg gcagggctgg tggggtgagc ataaaccgaa tgctttccgc
     2521 gtttaccgtt attctgctgg tggtctgtgg tgcgcttagt ctggggctga atcattaccg
     2581 cgatagcgcc atcacctaca aagcgcagcg cgataaaaaa gccagagagc tggagctggc
     2641 aaacgcaacc attactgata tgcaggtgcg ccagcg
//
LOCUS       DAZKHK010000293         2617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKHK010000293 DAZKHK010000000
VERSION     DAZKHK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.295"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..591
                     /locus_tag="ACHOS5_005247"
                     /pseudo
     CDS             61..591
                     /locus_tag="ACHOS5_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1051..2264)
                     /locus_tag="ACHOS5_005248"
                     /pseudo
     CDS             complement(1051..2264)
                     /locus_tag="ACHOS5_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2322..2606)
                     /gene="sfaC"
                     /locus_tag="ACHOS5_005249"
     CDS             complement(2322..2606)
                     /gene="sfaC"
                     /locus_tag="ACHOS5_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S/F1C fimbrial major subunit operon
                     transcriptional regulator"
                     /protein_id="HFP9779891.1"
                     /translation="MNCQKQVSHLLSFCLYREDDDMQNEIMGFLSRHNGGKTAEIAEA
                     LAVTDYQARYYLLLLEKEGMVQRSPLRRGMATYWFLKGEMQAGQSCSSTT"
BASE COUNT          621 a          630 c          645 g          721 t
ORIGIN      
        1 taatttttac agactgataa agttttgtct gctgaatctt tatacggaat tcttttcagt
       61 atgtgtacac gaattgactc tatggcggat aaatactgtt ttaccttggc ggaattaatc
      121 ctcacgtctt cgccagcagt ggcaggatgt cctggaggag tgtcggcaga ctgaggcggg
      181 gcagaaaaac ggctgcatat tgcactgctg aatgtggatt acgtcaccag ttttgaactg
      241 tcttttcgtc tgcttttaac ccggacgcca cagctgattg ttacacttcg ggaagcatgg
      301 gatatcagcc agaaaaatgt ggtgttcaac gataaacggt ttggctgcgt gtacagcctg
      361 aaagccagtc tttctggtgt gccggatata ttccggtatc atctgtctca tcgtattcgc
      421 cggatggttg ggagtgaaaa tacatcatcg ccatggcagc aaattgcccg ggaagtgaaa
      481 gcgccccgtg aacgactgaa gtatgcgctg gaaacgggat tgcaggtgat cgcactgaac
      541 ggcctgtttt ggtcagacag tcagcgtctg gtggtggata ttctgaggct aagcgccaac
      601 agacgtgata caggaaatcg tccgcatccc catttaccgt cttatatcac tactggcgct
      661 gacactgaaa gcatggtcgt cagagactct gtctggcaac caagggagca gaggccggag
      721 gtaattgaca ggagccttgc gccgggttac gtatgttctc cccggacaga caggctcagt
      781 ttgtagaatc tacaaattac tactactgat gctgccgggg aaaggcgtaa cgaaaaaaca
      841 gcctccgtta ccagatagca aggaggctga atggagttta taggatttgc gtttttataa
      901 tgtctggcca tgcggtaaaa ccggacaggt tttattatca tgtgaggtat tctgacataa
      961 aatgctggat ttttattttg tgacgaatgc tgcaaaattg aatctgcact ctgatgtaac
     1021 ttttatcttt ttcagtgaag tatgtagtgg tcaagtaata ctggccacgg ttttacagta
     1081 aaaatggtac ctgttcccgg actcttccgg tgtcaaccca ccgttataat ggtgaggtct
     1141 gacactgttg tagtagttca atatgtaatc attaatttgc tgccgggcct cgtccttgtc
     1201 gacgtaacca ttcgctggca cccattctgt tttcagactg cggaagaagc gttccattgg
     1261 gctattatcc cagcagtttc cccgccgact gacactttgc tttattctgt aacgccagag
     1321 aatttgttga tatttcaggc ctgtatactg gcttccctgg tcgctatgga acataacatc
     1381 atgtggttga ccacgcgtct cataggccat ccgcagggca tcgcttatca gtgcagtatc
     1441 ggcatgtgcg gacagactcc agccgataac cctgcgggca aaaagatcca tgacgaccgc
     1501 cagatagcac cagcaatttc cggcccagat ataggtaata tctccacacc atacctggtc
     1561 tggctcgggg acagcaaact ggcgcccgag cagattcagc aagcaggtat gctcctgacg
     1621 ggcatttttg tactgatgtt ttccgggctg acaactgctc agattcagat gtttcatcag
     1681 ccgcccggca cggtaacggc tcatcgggac gccgttttgg gtcagcatgt cagccagcgt
     1741 gcgcgccccc gcagagcccc ggcgttggtt ccacacccgg cgtatttcac tgcacaacct
     1801 gacatgcacc ggattcaccg tatcgcgtcg tttttgccag taccggtaac tgctgcggtg
     1861 tattcacaga gcagaacaca gactgacaac ggtgtggctg ccactgagtc tggcaactat
     1921 cgtgaaccgt tcagcgagtc ggacatcaag agtgcagtag ccttttttaa tatcgtattt
     1981 tgttcctcca ggcggcgaac ctgcttttcc agctcgcgga tacgttgctg gtctggagta
     2041 atcggtgtgg cagagggggc aatcccctgg cgctctcgtc tgagctgacg tacccagctc
     2101 tcaagagtgg tagagccgac attcatcaat tcactggctt gtcgatatga gtagccctta
     2161 tcaacaatca gttgcgcaca ttccagtctg aactcggggg tgaaagtacg tttggttttc
     2221 ttgttcatta agtcacctgt tttgtgttga ggtgagaata tcacctttaa tcaggtggcc
     2281 aaacttactg tgccactaca gtatgcccac agattgagtt attaagttgt ggaagaacag
     2341 ctctgtcccg cctgcatctc tcctttcaga aaccagtatg ttgccatgcc tcgccttaat
     2401 ggagagcgct gaaccatacc ttctttttcc agtaataaca ggtaatagcg tgcctgataa
     2461 tccgttaccg ccagcgcctc cgcaatttct gcggttttcc ctccattatg tctgctcaga
     2521 aaacccatta tttcattctg catatcatca tcctctctat ataagcaaaa ggataataag
     2581 tggctgactt gtttttgaca attcaaatgt gattatc
//
LOCUS       DAZKHK010000294         2593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKHK010000294 DAZKHK010000000
VERSION     DAZKHK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.296"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1678
                     /locus_tag="ACHOS5_005250"
     CDS             <1..1678
                     /locus_tag="ACHOS5_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HFP9779892.1"
                     /translation="TTNSLAAVWRNGQSELIPNALGKFLTPSVVCVDEDGMVLTGEAA
                     RDLQLIKPQNCASNFKRMMGTSKTLKLGGREFRAEELSSLILRQLKEDAENYLGEEVT
                     EAVISVPAYFGDMQRKATKAAATMAGLNVERLINEPTAAALAYGLHNKDDEHQFLVFD
                     LGGGTFDVSILGLFDNIMEVRASAGDNFLGGEDIVDILIDAYCSRRDLPENIEWREPT
                     LQRHLRIEAERVKRVLSVRDEATFSVEIEGRRYYWHLTTEKFEFLLQTFFERIHMPLE
                     RAIRNAKINISQLDQVVLVGGTTRMPLIRKLVTRLFGRIPAMHLNPDEVIAQGAAIQA
                     ALKARHSELRDVVVTDVCPYTLGVDTSKSLGHTRESGYFAPVIERNRSIPCSRVRAFY
                     TAHDQQTEVNFKIYQGESRMVADNIFLAELSVNVPPKPAGDVRIDVRFTYDINGILDV
                     DISVPLTGAKNSLVIEQNPGALTAEQIQQSLSKLSLLKIHPRDEQINQAFIARLDNLY
                     QLTLAETRDWISNCSRHFSYLLEKQDPDQLADFRTKVEPTLDSLERERLQ"
     gene            1675..>2593
                     /locus_tag="ACHOS5_005251"
     CDS             1675..>2593
                     /locus_tag="ACHOS5_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9779893.1"
                     /translation="MNCWEILGLQPGAEERDIKRRYAQLVKNCRPEDDPAAWQQLHDA
                     YEQALRYDDEDQWQEEEEEQPINMPRGIVYPRPVMTFRAPNTFSGHLLPDVAVKPEGE
                     FTWQAVLDMLCEENEATPEHAPMQLAKALSFLEKKDLSSRMCFEEALLLYLHRVFRPL
                     LTLAAAQTFRWHLVAGSRQPVIQTEINESCLLYYRIEKEIASYFYSTMSPSEELENGK
                     ALLSSYQQLHEDDRSHKWFKVALLNLINKYDLSDDYLSLIIELIGWSFQPDPHQFCWQ
                     VSEQFYVLFNLHKTGYEKILFALNLEQGKY"
BASE COUNT          745 a          534 c          607 g          707 t
ORIGIN      
        1 tacaactaac agtttggcag ctgtctggcg aaatggacaa agtgaactga tccccaatgc
       61 gttaggtaag tttttaacac ccagcgtagt ttgtgttgac gaagatggta tggttttgac
      121 aggggaggca gctcgtgacc tgcaactcat caaaccacaa aactgcgcat caaattttaa
      181 acgcatgatg ggcacatcaa agacgttaaa actgggcgga cgggaatttc gtgcggaaga
      241 actctcatcg ttgatattac gtcagctaaa agaagatgca gaaaactatt tgggcgaaga
      301 agtcactgaa gcggtgatta gcgttcccgc atattttggt gatatgcaac gtaaagcaac
      361 aaaagccgct gcgacaatgg caggcttaaa tgtcgaacgt ttaattaacg aaccaaccgc
      421 cgctgcactg gcgtatggtt tgcataataa ggatgatgaa catcaatttc tggtattcga
      481 tttaggtggt ggtacgtttg atgtctccat actggggttg tttgacaata ttatggaagt
      541 acgtgccagt gccggggata actttttagg tggtgaagat attgtcgata tcctgatcga
      601 tgcatattgc tcacgtcggg atcttcctga aaatattgaa tggcgtgaac cgacactcca
      661 gcggcattta cgcatcgagg ctgagcgtgt caaacgcgtg ctgagtgtgc gagatgaagc
      721 cactttcagt gtggagattg aaggaaggcg atattactgg catctgacta cagaaaaatt
      781 tgaattttta ttacagacct ttttcgaacg aatccatatg ccactggagc gggcaattcg
      841 caatgccaaa attaatatta gccaattaga ccaggttgtt cttgtgggtg gcactacacg
      901 tatgccactg attcgcaaac ttgtgacacg attatttggt cgtataccgg caatgcattt
      961 aaacccggat gaagtcattg cccagggcgc ggctattcag gcagcactta aagcgcggca
     1021 cagcgaatta cgagatgttg tagtgactga tgtttgccct tatacgcttg gcgttgatac
     1081 ctctaaatcg ctggggcata cccgcgaatc aggctatttt gcacccgtta ttgagcgtaa
     1141 caggagtatt ccttgtagtc gcgttcgtgc cttttacacg gcacatgatc aacaaacaga
     1201 ggttaatttt aaaatctacc agggtgaaag caggatggtg gctgataata ttttccttgc
     1261 ggagttgtca gtgaatgttc cgccaaaacc ggccggtgat gtgcgtattg atgttcgttt
     1321 tacgtacgac attaacggta ttcttgatgt cgatatcagc gtgccactga caggtgctaa
     1381 aaatagtctg gttattgaac agaatccagg ggcattaacg gcagaacaaa tacagcagtc
     1441 gttgtccaaa ttaagcctgt taaaaattca cccacgcgat gaacagataa accaggcctt
     1501 tattgcccgt cttgataatc tctatcaact caccctggcg gaaacgcgag actggataag
     1561 caattgctcc cgtcatttca gttatctact cgagaagcag gatcctgatc aacttgctga
     1621 ttttcggact aaagttgaac ctacattaga ctctctggaa agagaacgtc tgcaatgaac
     1681 tgctgggaaa tattaggttt acaaccaggt gcggaagaac gagatattaa gcgtcgttat
     1741 gcgcaactgg taaaaaattg ccgcccggaa gatgatcctg cagcctggca acaattgcac
     1801 gatgcttatg aacaggcttt acgttatgac gacgaagatc agtggcaaga agaggaagaa
     1861 gagcagccaa tcaatatgcc caggggtatt gtttatcctc gtcctgtgat gacatttcgc
     1921 gcacccaaca ccttttctgg tcatctactt cctgatgtcg ctgttaaacc agagggggaa
     1981 ttcacctggc aagccgtgct ggatatgctc tgtgaggaga atgaagctac ccccgaacac
     2041 gcccccatgc aacttgctaa ggcgttatct ttccttgaga aaaaagatct cagctcacga
     2101 atgtgctttg aagaagcgtt attactgtat cttcatcggg tatttcggcc attattaact
     2161 ctcgctgctg cacaaacgtt cagatggcat ttagtcgcag gcagtcgtca acccgtaatc
     2221 cagacagaaa ttaacgaaag ttgtcttctt tattatcgca tcgaaaaaga aatagccagt
     2281 tatttttaca gtacgatgtc accttctgaa gagttagaaa atggcaaggc gctgttgtct
     2341 tcttatcagc aactacacga agatgacaga agtcataaat ggttcaaagt ggctttatta
     2401 aacctcatca ataaatatga tttatctgat gattatttaa gtttaattat tgagttaata
     2461 gggtggtcat ttcaacctga cccacaccag ttctgctggc aggtatcaga acagttttat
     2521 gtattgttta atctacataa aacaggatat gaaaaaatac ttttcgcctt aaatcttgag
     2581 caagggaaat atc
//
LOCUS       DAZKHK010000295         2574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKHK010000295 DAZKHK010000000
VERSION     DAZKHK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.297"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..167
                     /locus_tag="ACHOS5_005252"
     CDS             <1..167
                     /locus_tag="ACHOS5_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779894.1"
                     /translation="VTAEEIIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEI
                     LAYRVGWAQE"
     gene            705..2330
                     /locus_tag="ACHOS5_005253"
     CDS             705..2330
                     /locus_tag="ACHOS5_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779895.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSELPI"
     gene            2327..>2574
                     /locus_tag="ACHOS5_005254"
     CDS             2327..>2574
                     /locus_tag="ACHOS5_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779896.1"
                     /translation="MSKKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATG"
BASE COUNT          649 a          562 c          718 g          645 t
ORIGIN      
        1 cggtgacagc agaagagatt attgcgctga gtgaagcggc agagcaggcg atgttcacca
       61 aagggatgga aatccacgtt cgccagcgca ccatgaagaa agagctggaa aagctgacca
      121 gtgcggatga gatactggca tacagggttg gttgggcaca ggaataacag acaaaaagat
      181 gggggaccat cacctccccc atgaactgag tcaattatac acttgtgaag tatttactat
      241 gcgaataaat actgtcacgg aaagtgcagg cctggatgtg atggaaatca tgtttccggc
      301 gcatcctgtt gatatggata atttttcaga tttccggaag tgagagtttt tggtgtttcc
      361 ataccagcca cagttgatgt gtgttgtggc gcctggcagt gcgattaaag gagcagaggg
      421 aataaaaaac gggggcatga ccaacccccg gtacaaggaa ggagtaacta caatggaaaa
      481 attaatgaag acctttagtg ctacgttata tggcgaataa agactgccac agggaagttt
      541 gcagaaacag tgatgccgat cgtgtttctg acgctcttct ctgataagag taatttttca
      601 gatttctgga aatctgtatt ctgcacaggc gcaattgtat gccgccttta aaacttcaat
      661 tcagtgactc acctgccatt caaattttcg gataccagac aaccatgcct tatatcgata
      721 taacaactat gcgcgggatg atgccaggcg ttattgcatc tatgctgcca gatcattctg
      781 ctgtactggc agaaaactgt cattttcgct atggagtgat cacgcctgaa caccagatgt
      841 cagaggctga gaaaacattc gcgattaagc cgaaaaccat ttttcattac cgtgacgatt
      901 tctggtttgc atggacggat gtggtggatg tgatccgcag tccggtcgct caggactccc
      961 acgggcgtat ttactacact gacgggcgtt ttcctaaagt gacggatgcg accattgcca
     1021 caaaagggga cgggaatcac ccgacatcat cgtatcgtct ggggatcccc gcgccgacga
     1081 cagcacctgt ctgtactgtt cagcagggcg gtgatgtttc tgacgataac ccgaatgatg
     1141 acgaaacccg gttttatacg gaaacctttg tctcagatta tggtgaagaa ggtccgccag
     1201 gtccggcgtc tctggaggta acactccgta ctccggggac tgcggtacag ctgacgctgt
     1261 ctccggtgcc attgcagaat gccagtatta aacgccgccg gatttatcgc tctgcatcag
     1321 gtggaggaga agcggatttt ttacttgtgg ctgaactgga tgcatccgtg ctcagttaca
     1381 cggacaaaat accggggaaa aaccttggac cttctctggc tacatgggat tacctgccgc
     1441 cgccagagaa tatgacaggc ctttgcctga tggctaatgg tattgccgcc gggtttgccg
     1501 gtaatgaagt gatgttttcg gaagcgtatc tgccgtatgc atggccggaa gtgaatcgtc
     1561 acacgacggc agaagatatt gtggctatct gtccgctggg aacgtcactg gtggtggcga
     1621 caaaggggga gccttatctg ttcagtggtg tatcgccttc caccatttct ggctccagaa
     1681 ttccttccat gcaggcatgc ctgagccgaa gaagtatggt ggcgatggag ggattcgtac
     1741 tctatgccgg gacaaacggt ctggtatctg ttgatgtaaa cggtaataca gcactggcaa
     1801 cggaaaagat tatttcacct gaacagtggc agagtcagtt taacccggcg tccattgtgg
     1861 cttattcctg gcgtggtgag tacattgcct gttacacgaa accggatggt aagcaggatg
     1921 tgtttgtatt cagtccggtg aacatggata tccgttatct cagtacaccg tttgactgcg
     1981 catgggttga tctcgcgaaa gatatgatgc gtgtggtgac aggagacaaa atgtcagtgc
     2041 ttgccggggg ctctctgccc tccacgataa ggtggcattc aaaaattttt tcattacctg
     2101 aaagaacctc tttttcctgt atcagggtga aatctccggc gcctgagcgg gtggggatca
     2161 ccattatggc tgatgatgtt cctgtgattc attttgcgcc gggtacgttt aagggaagtg
     2221 tggtgagact tccggcagca accgggcaaa actggcaggt gatggtatcc ggattcgggc
     2281 aggtggaacg aataaccctg agtacatcga tgtcggagtt accgatatga gcaaaaaacc
     2341 gtggcgggcg gggaaggatt tatccactgt tgtggagaat atggaaattg gcaccgggca
     2401 gcgtggtgac ggacgtcacg cgtttgtgac ccgtgaagaa ctggtaggtc ttaaactcgc
     2461 ccggcgtcga acatcgggtg gtgcctcata tgcactgaat ccgggtattg agattgacag
     2521 tactttaatg actgttgatt ttcccacaaa accgctgaat tttaaggcga cagg
//
LOCUS       DAZKHK010000296         2567 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKHK010000296 DAZKHK010000000
VERSION     DAZKHK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.298"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            37..423
                     /locus_tag="ACHOS5_005255"
     CDS             37..423
                     /locus_tag="ACHOS5_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779897.1"
                     /translation="MRKIKTKIYMPTALTGNMDKHYIPVLEDLRKAIYSDRMLSRLAD
                     SDNIVIHSSVGYPVAKYKNTGISVGIEPLNPMIRQDLTLGYIVVVRNGKASQEINGLL
                     NRSLPKAISTFKDHINEYEAAKSKMR"
     gene            479..1459
                     /locus_tag="ACHOS5_005256"
     CDS             479..1459
                     /locus_tag="ACHOS5_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779898.1"
                     /translation="MEGKLIFCSDAILRFQSDYDETSAVPLLSIQNAIADAAPFFLLR
                     FFRHTALIEDGTTLASIFLAIEPWKELLAAYLDRDVGAYIDEVRKPSGPMTWDIEWIG
                     IDHRSSVYRAYKRQDMEEGEDFSTYFNRERFPTDEFDIESSCDASGFIKGDKERWSIS
                     GDVQQIKNLPVILYSKQTLMTSSKDGLLKKNVSGVKSSKHGCFVYGDTSFSFSEVMEA
                     IFISGLFFYAPKDAASSLDELKASLAELEEERAENPNAESTGNETDEESTIVVAEGAF
                     DSLAAHMESEKAEWQSIKKLCQQDSELPIRIGSIKLAEPPELRLCGKIIH"
     gene            1810..2397
                     /locus_tag="ACHOS5_005257"
     CDS             1810..2397
                     /locus_tag="ACHOS5_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779899.1"
                     /translation="MSYDYEKLENKKNEIIGIVHELFTENDKTAISLVDSFCRLTPPE
                     KEKFVMEYITVNSLGRRGGKSRKLGNFVLNWRELFNDLPDLVLTAAGVAAEPKLIFFA
                     ALSIWNKIWCHSSIELTPEHATVLYVLWQGRDESNKIRRDDAFAKSGIQFNFYGMQEL
                     VDVSFDDIINDLVKMRCIEVNDNIIWLREWVRGTY"
BASE COUNT          846 a          409 c          568 g          744 t
ORIGIN      
        1 catggtatta aataaaaaca ataacaggaa aatatcatga gaaaaataaa aacaaaaatc
       61 tatatgccta cagctttaac aggaaacatg gataaacact atatccctgt tttagaggat
      121 ttaagaaaag cgatatatag cgacagaatg ttatcaaggc ttgcagatag cgataatatt
      181 gttatccata gttcagttgg gtatccggtt gctaaataca aaaatacggg gattagcgta
      241 ggtatagaac cattgaatcc aatgataaga caagatctga ctcttggata tatcgtggtc
      301 gtcagaaacg gaaaagcatc acaggagatt aatggtttac ttaacaggtc attgccaaaa
      361 gcgataagca cttttaagga ccatattaat gaatatgaag cagcaaaaag taaaatgcga
      421 taacaatcta tgatctggta aaatattttg tctgataact acaggaggag gatgagcgat
      481 ggaaggtaag ttaatttttt gttccgatgc aatactgcgg tttcagtccg attatgatga
      541 aacctcggct gttccgttgc tttccataca gaacgcgata gctgatgctg cccccttctt
      601 tcttctccgt ttctttcgcc atacggccct gattgaagat ggaacaacac tggcaagtat
      661 ctttttggct atagagccgt ggaaggaatt gttagcggct tatctggaca gagatgttgg
      721 tgcctacata gatgaggtaa gaaaaccgtc cgggccaatg acatgggata ttgaatggat
      781 tgggattgac catcgaagct cggtttatcg agcttacaag cgtcaggata tggaagaagg
      841 ggaagatttt tcaacctatt ttaatcgcga gcggttcccg actgatgaat ttgacataga
      901 aagtagttgc gatgcttcag gcttcattaa aggtgataaa gaacgctgga gtatcagcgg
      961 cgatgttcaa caaattaaaa acctgcctgt aatcctctat agcaaacaga cattgatgac
     1021 ttcgtcgaag gatggattgt tgaagaagaa cgtatcaggg gtgaagagtt ccaagcatgg
     1081 ctgctttgtt tatggcgata cctcgttttc tttcagtgag gttatggaag ccatcttcat
     1141 tagcgggctg ttcttctatg caccaaaaga cgccgccagc agtcttgatg agctgaaagc
     1201 gagtctggct gaactggaag aagaacgcgc tgaaaatcca aatgcggagt cgactggcaa
     1261 tgaaacagat gaagaatcca ccatcgttgt agccgaaggt gcatttgatt cgctcgctgc
     1321 tcacatggag tctgaaaaag cggagtggca atccattaag aagttgtgcc agcaagatag
     1381 cgagctgccc atacgtattg gtagtattaa actagctgag cctccagagc tacgactgtg
     1441 tgggaaaatt atacattaag taagcatggt gcctaccgaa gtctatgctt gattactatt
     1501 tttacgttag tcctatttgg ggaactaatt ttcaaaccga gtagtctatt tcaaccaatc
     1561 taagtagtga ttgcgatcga tgagctaaca ttatcgcatt taattttgta ttgttacatt
     1621 tttataacag tgatgccaaa gacaacgatg gaaattcatt aagcattttc agagcagcca
     1681 ttaatagaac tggtagacta tacaatcatt taattatttg tttaatatgt tgaaatactg
     1741 aacaaaatat gctaaatagc cttagggaca aagatacctg tgttagtaat caaaaggact
     1801 atgtgacaaa tgagctatga ctatgaaaaa ttagaaaaca aaaaaaatga gataattggt
     1861 attgttcatg aactatttac tgagaatgat aaaaccgcta tttctttagt cgattcattc
     1921 tgtagattaa ctccgcctga aaaagaaaaa tttgtaatgg agtatattac agtcaactca
     1981 ttaggtcgac gtggtggaaa atcaagaaag ttaggtaatt ttgttcttaa ttggagagaa
     2041 ttattcaatg atctacccga tttagtgtta acagcagcag gtgttgcagc cgaaccaaaa
     2101 ttaatatttt ttgcggcgtt aagtatttgg aataagattt ggtgtcattc gtctatagaa
     2161 cttacaccag aacatgctac tgttttgtac gtcttatggc aaggaagaga tgagtctaat
     2221 aaaataagga gagatgatgc gtttgcaaag agtggcatac aattcaattt ttacggaatg
     2281 caggaattag tggatgtatc attcgatgat attattaatg atctagttaa aatgcgatgt
     2341 atagaagtta atgataatat aatatggttg cgtgaatggg tcaggggaac atattaacat
     2401 gcaactgtat tatacggata aacaagcctc gaaagatatc tgggcttgtt tgtatatggg
     2461 gttgaattca gggatgaggt aggtctgagc taaaatatat agatatagcg gaaccctcat
     2521 aaaaaacaaa tgttcatttt acaatgccat tttttaatac ccatttt
//
LOCUS       DAZKHK010000297         2548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKHK010000297 DAZKHK010000000
VERSION     DAZKHK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.299"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1271
                     /locus_tag="ACHOS5_005258"
     CDS             <1..1271
                     /locus_tag="ACHOS5_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP9779900.1"
                     /translation="PMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESATLP
                     VITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLTLV
                     LPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVLSL
                     DMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAETW
                     FDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQLY
                     RDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSPIL
                     FTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTVYT
                     GGHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(1413..2537)
                     /locus_tag="ACHOS5_005259"
     CDS             complement(1413..2537)
                     /locus_tag="ACHOS5_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP9779901.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          779 a          575 c          483 g          711 t
ORIGIN      
        1 tacctatgaa aaactatctt ttccagacac ccgaagatat ttgtgtacag ttaaaaaaaa
       61 tgacacatcc tgtcacaata agaacaacag atattgctaa tttctggcac tatcttgagt
      121 cagcaacact tccagtgatc acaaaaagca ccactacaga aaatcgggag gttacatttc
      181 tgtggcgctc agagaaagca ctgcaaggcg tatatcttcg cctgaatcgt gtaacagata
      241 aaaaagacgt caaaaaagga ctaatgactc atatcccttc gacagatatc tggatgctga
      301 cactggtgtt accagcttca tatcggggct catactcatt tatagaaatt cccacagata
      361 tgacacaaaa agacatattt caactaggaa gtcgcttctc tccattaccc ggtaaatctg
      421 atccatttaa caaaacagca gaaataaata tacgaggatt cggagaatca gtcctttctc
      481 ttgatatggc tcctgaacaa aaggaatggg atgatacttc ccataaatgt acaggtattc
      541 tttcaacatt acattccttt gttgcaggat atcaacgccg gattcgttta tattttcccc
      601 tgaatccaac atcagtacct cttggattac ttgtgttacc tgatgctgaa acatggtttg
      661 accggataga tattacccgg gcattagata tggccatcac cactggtcgt atcacgccaa
      721 tggcaattat ggggatagac aatattaatg aatctgatcg tatgaatata ctgggaggca
      781 ataaagaact catctttgat atagcggaaa atctgatacc ccagttatac agagactatc
      841 cggatatcgt atgggctggt cgctctaata ctgtactggc cgggcagagc ctcggtggag
      901 tgacagcact gatggcagct atatatgcgt cgacaacatt tggtacaatc attagtcact
      961 caccttcaat gtggtggaac cctgaccagg gcagcccgat cttgtttact gagaataata
     1021 cctcctgggt aagtgagcag atactttcag cgcctccgaa agatgtaaat attcgacttg
     1081 gagtcggttc tttagaaggt acaaccgtct cacatgttca gcgattgcat cattcgttaa
     1141 tcgcagctgg tttggaaagt aacctcactg tctataccgg tggtcacgat tatgcttggt
     1201 ggcgcggagc aattattgat gaattagcaa attataattg caggaaggta tcaaataata
     1261 actctgtgta aaatatcttt ttatcgtccg ataactcttt ctgcaatatt attatgggag
     1321 actatctcat ttatttagtg atgtccttca tttatgtgat gaaggacatt atattactca
     1381 taacatttct cgttgaaaat ttcagcaata cgctacaatt gtttcccgtt gttttttaat
     1441 tttctcatca aaacctctgc aatctctgca ggactgggtt gttcagccat ttcagcggct
     1501 acctgatctg aacagaagcg tagtgaatca tcataaagca ggcgatttac caaatcacta
     1561 gtcagtccat gcttgtccgg aataatccca cacccacgct tcgcaacaat ttctgcatta
     1621 acagagcgat ctgcaccttc gccaaacaca atctgtggta ttccactata cagcgctgtc
     1681 agggtattac ctgcgccacc gtgatgaata aatccatcag ctccattaag gaatacaccc
     1741 ataggtatcc agtcaaccag acgtacattt gatggtagtt ttcgtaaacc agtacgagca
     1801 tttattgcaa gttgcaaaat gatatcagca tcaacttcat ttgcagaatc cataacccat
     1861 gaaatcagct ccagaccatc aaccattggt tttacagtgc caaggctgat aagtaaacgt
     1921 ttccgatcag aatccctgtc ccaccatgtt tcctttacag cacctccgtt ataaggaata
     1981 tatctcattg agataaccgg ttctccagca tttttaagaa tgctcatact tgggggagca
     2041 acatcaatcc atgctaaatc acatagtgga ccgctgactc catgccgcct gtaagcattg
     2101 cttaaagaac ggtttaacat ctgaatatgg gcagatgtat gcgcgaatcc aacagccagc
     2161 atcactgaag gaattctata tttagcagca accaatgggc ctgccggacc aagaggggga
     2221 tagactatta aatctggcct ccattttcct gcaaaatcga tgaggttatc tgccatttca
     2281 tcgctaaaaa aagagaaatg accaataata ttactttttt tcctcaactc ttcctgatgg
     2341 cgatatccag cctctgaatc taaacctgga actgcatcaa aaacaaccag tccagcttct
     2401 gctgctttat tcgcgaattt gccagcgcta ctaattacta catcatgtcc aattacacga
     2461 aaggcctgag ccagagaaat caatgggtat aacaaaccgt acagcggtgg gccaataaaa
     2521 agaatacgtt ttcgcattaa aaaagtcc
//
LOCUS       DAZKHK010000298         2475 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKHK010000298 DAZKHK010000000
VERSION     DAZKHK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.300"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..874
                     /locus_tag="ACHOS5_005260"
     CDS             <1..874
                     /locus_tag="ACHOS5_005260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779902.1"
                     /translation="LSALAKENGIDVKSDVGKSLFFLCVGKNKGWRKVQKASDYGVII
                     LTEEQFINFIDTGEIPLEQDCVVENDISKSLEKDVNTYKSMIDEFSLSIRTVREFRRS
                     NALIAVFVDGYAAGWRFAVKECYKDSLDIKLTPVKTEFFNYEIWTQGDSFQFNRGDVF
                     YSDRLAYDSYCDFLKLDDAVVLQVKYECFSGYDSVSTFDGVMDGEFSFSRMEPRPVHK
                     LPVLIETQLYDPGKIIVDVFVPDAAKKQLKLKDTLELKQDEFVCLLQSGCCWVKRREA
                     GDKKKPEFINLFKK"
     gene            complement(989..1129)
                     /locus_tag="ACHOS5_005261"
     CDS             complement(989..1129)
                     /locus_tag="ACHOS5_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9779903.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            1393..1596
                     /locus_tag="ACHOS5_005262"
     CDS             1393..1596
                     /locus_tag="ACHOS5_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779904.1"
                     /translation="MKMILSRKKIEIEVVQSGTADNPETVTKIKVSRPEDLEACGVIV
                     EKICAIHAKSVNTTASNDHNKYN"
     gene            complement(1713..1853)
                     /locus_tag="ACHOS5_005263"
     CDS             complement(1713..1853)
                     /locus_tag="ACHOS5_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9779905.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHTSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            complement(2010..2300)
                     /locus_tag="ACHOS5_005264"
     CDS             complement(2010..2300)
                     /locus_tag="ACHOS5_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779906.1"
                     /translation="MQDFAKVCKNSGFLRIQKNFNTLNNKRFLKLRHDRRSEKGSGDE
                     NEKIRKARTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
BASE COUNT          653 a          516 c          580 g          726 t
ORIGIN      
        1 cttaagtgct cttgcaaaag aaaatggtat tgatgttaag tctgatgttg gaaaaagttt
       61 gtttttcctt tgtgtaggaa agaacaaagg gtggcggaaa gttcagaaag ccagcgatta
      121 tggcgtaatt attctgacag aagaacaatt tatcaatttt attgatactg gtgaaattcc
      181 tcttgagcaa gactgcgtgg ttgagaatga tatcagtaaa tcgcttgaga aggatgttaa
      241 cacttacaag agtatgatag atgagttctc gctttctatc cgtactgtaa gggagtttcg
      301 ccgtagtaat gcgttgattg cggtatttgt tgatggttac gcagctggtt ggcgctttgc
      361 tgtaaaagag tgttacaagg attctttgga tataaaactt accccagtaa aaacagagtt
      421 ttttaattat gaaatttgga cgcaagggga ttcctttcag tttaatcgcg gtgatgtttt
      481 ttatagtgat cgcctggcgt atgattctta ttgcgatttt ttaaagcttg atgatgctgt
      541 tgttcttcag gtgaaatatg agtgcttttc tggttacgat tctgtaagta cttttgatgg
      601 tgtgatggat ggtgagtttt ctttttctcg tatggaaccc agacctgtcc ataaattacc
      661 tgtgctgatt gaaactcaac tgtatgaccc cggaaagatt attgttgatg tttttgttcc
      721 tgatgctgcg aaaaaacaat taaaacttaa agacacctta gagctgaagc aggatgaatt
      781 tgtctgttta cttcagtcag ggtgttgttg ggttaaacga agggaagcag gagataagaa
      841 aaaacctgaa tttattaacc tattcaaaaa ataaaaatat agcccgcata tcgcgggcaa
      901 atgtctatgt aattcaacgg agaaagggtg ccatatcgac cgaacccggc aaccggatag
      961 cgggggcttt cacccccgct gcggtttctt acggtttaca ctgcaagaac gccgcaaatt
     1021 ctgaccacat gatgttaagt ctgaactcac acggagaacg gtgtatcatc cagacggtca
     1081 acaacatggt taagcataaa atgctcacga tgatcgtttt ttgcggcata gcgcttgact
     1141 ccttttacag ggaggcgcta accttccact tgtcacggta ggaagtgtta gggcctcggg
     1201 ttaacattta agttgactcg gggcctttcc acatccggcc ttcaggtaat ccttccagcc
     1261 atcagccgaa aggcacccgc gtgtaatgta ctgatttccc tttgtgtgcg caatgcctgg
     1321 cgttgaagca gacaacgcta tcgcactggt gatattcttt cgcacaggct tactctgtcc
     1381 tgtgtcgggg cgatgaaaat gattctttca agaaaaaaaa ttgaaattga ggttgtgcaa
     1441 agtggaacgg ctgataatcc tgagacggtt acaaaaataa aggtttcacg cccggaagat
     1501 cttgaagcgt gtggtgttat cgtggaaaaa atttgtgcta ttcatgcaaa atcagttaat
     1561 accactgcca gtaacgacca caataaatac aactgaacaa aaaagcccgc atattgcggg
     1621 caaatacgtc tgtaatccaa tggagaaagg ggtgccatat cgaccgaacc cggcaaccgg
     1681 atagcggggg ctttcacccc cgctgcggtt tcttactgtt tacactgcaa gaacgccgca
     1741 aattgtgacc acatgatgtt aagcctgaac tcacacggtg acgtgtgtat catccagaca
     1801 atcatcaaaa tagttatgca taaaatgctc actatgatcg ttttttgcgg catagcgctt
     1861 gactcctttt acagggaggc gctaaccttc cacttgcgag gtagatgggt tagggcctcg
     1921 ggttaaacaa atgtttgact cggggccttt ccacatccgg ccttcaggta ttccttccag
     1981 ccgtcagccg aaaggcaccc gcgcgtaatt taccttttac gtccgttacg tgcaacggat
     2041 gacgtggctg tttttccttc cccttcgcca tcctttttcg gagcagttgt gcaaaagtgt
     2101 gcaaaagtga tttttgctgc cactccccgc caggccgcgc cagttctggc tttcctgatt
     2161 ttttcgtttt cgtcgccgga tcctttttca gatcttcgat cgtgacgtaa ttttaaaaat
     2221 ctcttgttat tcagtgtatt aaaatttttc tggatcctca gaaagccgga gtttttgcac
     2281 acttttgcaa aatcttgcac aaagtgcaaa accattcaga agggaaaacc tcagtaatgg
     2341 cgcgccctgg cggggtgttt tgcacccctt tcgatttgca aaatttatcg tacacaaata
     2401 ccggaggcgt gtgcggggta gcgccgatta cgtcacttta cgcgttacgt cgcccccgaa
     2461 aatttccggc cattt
//
LOCUS       DAZKHK010000299         2467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKHK010000299 DAZKHK010000000
VERSION     DAZKHK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.301"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(131..1657)
                     /locus_tag="ACHOS5_005265"
     CDS             complement(131..1657)
                     /locus_tag="ACHOS5_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9779907.1"
                     /translation="MSKKFTKTLLSSSLGGLLLGISSGVLAQELPPIKDSGLPFYSVL
                     KEADASSVIHGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAAGLRTSDKENGKHQVDEEEVRRIFNKVNNLSNTIINPEVLKQSD
                     FIASGPRPEGDPLVQASAVGEFALAVGEGAAAEEKNSTAVGSWASAHGRQSTALGSGG
                     QAYANGSTALGSYSHVDDSSVYGVALGGSSVVGENAPEGTALGSRAEVSHRNSVALGA
                     RAHTTRDNEVYIGYVEEAGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQD
                     TNTRLVVEAKKSREYTDSRTTVGVNSDGTLTSADGATKTIAVNDGLVALSGRTDRIDA
                     AVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLF
                     QPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSYNVGVNFEF
                     "
     gene            complement(2017..>2467)
                     /locus_tag="ACHOS5_005266"
     CDS             complement(2017..>2467)
                     /locus_tag="ACHOS5_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779908.1"
                     /translation="WIDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEV
                     MPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDL
                     RKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVDRFNREYNKKVGVE"
BASE COUNT          630 a          682 c          477 g          678 t
ORIGIN      
        1 ccatactcca gataacggtt gcgggtcgcc agaatcactt tcatcgctgt cacctgtttc
       61 atgctcacat gagaaaaaac ggcaggcaaa actccacgcc tgccgtcatg cttcatgtca
      121 ctgtcagcaa ttaaaactca aagttcacac ctacgttgta ggacacatcg ccatcactcg
      181 ctgccacacc tgctttcgct gctgtctgct cattgaaacg gtaaccaaca cccacggcaa
      241 cagcctgttt atcactgtac ccccccaggg cagcagtggc gttgaatttc cccacactgt
      301 acggctggaa aagccccgcc agcgctgcac tctgtgctgc tgcacgcttc atctcttcgt
      361 gattctcacg aatttgacgc tgctggctgt tcaggcgtgc attatgttcc tgcaactggc
      421 gagtattgga ctgaatagcc tgtgtatttt tagtaacccg gcggtcaacg ctaccaactg
      481 cagcatcaat acggtctgtt ctgccggaca gagcaacgag accgtcatta acagcaatcg
      541 tttttgtagc gccatcagcg ctggtcaggg ttccgtcaga gttcacacca actgtcgttc
      601 tggaatctgt atattcacga gactttttcg cttcgactac aaggcgggta ttcgtatcct
      661 gagccagttg ctcaacactg tcttttacac ggtcaacctg acgaacggtt gctgcatctg
      721 agggacgagt accgtcactc aggccaccaa gcacacgagt tttataagcc ttgcccgctt
      781 cttctacgta gccgatataa acttcattat cacgggtagt gtgcgctctc gcccctaaag
      841 caacactatt tctatgagaa acttccgctc tgcttcccag tgcagtgcct tcgggggcat
      901 tttcacccac aacagagctg ccccctaatg ccacgccata aacggatgaa tcatccacat
      961 gagaataact tcccaacgcg gttgaaccat tagcataagc ctgcccccca cttcctaatg
     1021 cagtactctg tctcccatga gcactagccc atgaaccaac agctgttgaa ttcttttcct
     1081 ccgccgctgc accttctcca acagccagag cgaattcgcc caccgcacta gcctgaacca
     1141 aagggtcacc ttccggacgt ggtcctgatg cgatgaaatc cgattgtttt aatacttcag
     1201 gattaataat agtatttgat agattattaa ctttattgaa gatccggcgg acctcctcct
     1261 catcaacctg atgcttaccg ttttctttgt cagacgttct gaggcctgct gccatacggg
     1321 cgaagtcttt tagcacaggc tcatgaacgg tatatgcaat tgagttatct ttactcaata
     1381 ctataatatt accctcctga tcgaaattaa aaagggtctc atcttttccg ttcagaacat
     1441 atcgcattgt tccccaacca gaggatttgt tatagtaaat acgataagta ctaccgtgta
     1501 taacagacga agcatctgcc tctttcagca ctgaataaaa aggcaaacca gaatctttga
     1561 taggaggtaa ctcctgagct aaaacacctg agctgatccc aagcagaagc ccacccagag
     1621 aagatgacag aagtgtcttt gtgaattttt tactcattat ggatatcctg ttaaattatc
     1681 tgtatacaaa tcagccgcaa cacctcatac tcataaagaa atatgattaa ttacggcttt
     1741 attcaggata ttctgaaaat gatcgtgatt attaatttta aataactagg aattatctta
     1801 ctatattctt ggtgtattca ctcctgagac atagagcaac gtaggaaccg aaataagaaa
     1861 aatcttaccc tcatcaggca tgacatttca catctgctta cactgtaatt tctgatcaag
     1921 atcacaacat tatccgaatt taaatgttca gttcatctac aatatttccc tgcgtgactt
     1981 tatatcagcc ccgtacatcc gtacggggca tgttctttac tccactccca cttttttgtt
     2041 gtactcgcgg ttaaaccgat ccacggcaat tttcatatcc cgctcaaccg atttcaccat
     2101 cgccgactgt tgcgccagac tgagagtact gtcggcgtaa atggcatcac gctgtttacg
     2161 caaatccttc agtctcttcc gggtatcctg cataagcccg ttcatcgaca attttccgtt
     2221 gttctcgtca atgaacgccg ttctttctgc accggtcaga ctcttcagct ctgcgtgata
     2281 ctgcgcgacc tccgtcatcc ggtcgtacat cttctgctgg tcagcatacg gcatcacctc
     2341 acctgaaatt ttccccagga agggcacctg ctgctccggt atatcaatac cattcagcga
     2401 tttcaccgcc gcatccgtgg ttttagaaat gaagcgcccg gtcccgccag agatatagtc
     2461 aatccag
//
LOCUS       DAZKHK010000300         2442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKHK010000300 DAZKHK010000000
VERSION     DAZKHK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.302"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..322
                     /locus_tag="ACHOS5_005267"
     CDS             80..322
                     /locus_tag="ACHOS5_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9779909.1"
                     /translation="MFSLIQRGQIYTDRAGYPVVITRSTEHLVFFRRMDGHSGRVRIS
                     EFNNHFEHIDHQEFRQIQTELEQEAHLKKLRAMKRK"
     gene            478..693
                     /locus_tag="ACHOS5_005268"
     CDS             478..693
                     /locus_tag="ACHOS5_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9779910.1"
                     /translation="MALNIDCVPITTWCRDMGETLDAVNKRLQRGVWKEGVQVLNVEG
                     SKERWIDLTEVSKWARKNKYPYHSQEE"
     gene            648..1820
                     /locus_tag="ACHOS5_005269"
     CDS             648..1820
                     /locus_tag="ACHOS5_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9779911.1"
                     /translation="MGEKKQISLSLPRGITIRQYKNCSTLVVTFTYKGVLCREPLPRL
                     EANVKGIKYAERLLGEIKNQISDNSFDFSKYFPNSRKLALFGMVKKNKSVRDYLEEYL
                     EICKNRKLSPSTINGYEKCVSALSELHKLHVVDLTPAILKKWISKRTTKLKTIRNNLS
                     FLRSAVDEAVTDGLITINPVKLVSASRYHAVNHTANADDYEVDPFTPAEATAIYNACI
                     YQEWRNLFCFAFNTGLRSSELCALRWSDIDFIGHTVHVQVASVVGVIKGTKTKAGTRK
                     VELNNEAIMALQAQKSFTMMRSDFVFSDPKTRKPWANSAAIRKKAWIPTLKKAGVRYR
                     NPYQTRHTFATRHISQGANLFWLAGQMGHKGPEMLFRHYGSYLSEYDGKTAISEVI"
BASE COUNT          797 a          474 c          529 g          642 t
ORIGIN      
        1 cctgaagtgt tctcaaatgt tggtattgcg ttggcgattg tgctggttgt gtactcaatt
       61 tgccgctggg ggtaaataca tgttctctct gattcaacgt ggtcagatat acacggacag
      121 agccggatac cctgtggtga ttactcgcag cactgagcac ttagtgttct ttcgacgcat
      181 ggatggacac tccggtcggg tgcgcatcag tgaattcaac aaccattttg aacatattga
      241 ccatcaggaa tttcgccaga tacagacaga actggagcag gaagctcacc tgaaaaaatt
      301 acgtgccatg aaaaggaagt gaagagtaaa taaagcattt gaactatggg tccaccagcg
      361 ttatggcagt cgctatgacc tgacgcgaga tgttgacggt ttctactgcc gtgaagtggt
      421 gaaacgaatg tttgaatgtg gtgccactgc cgtggatgaa agttttatga ggtaactatg
      481 gctctcaaca ttgattgtgt acctataacg acatggtgtc gagatatggg agaaacactg
      541 gatgccgtta acaaacggtt acaacgtgga gtatggaagg aaggggttca ggttttaaat
      601 gtggaaggct ccaaagaacg atggattgat ttaactgagg tttcaaaatg ggcgagaaaa
      661 aacaaatatc cttatcactc ccaagaggaa taaccattcg tcaatacaag aattgctcta
      721 cattagttgt aaccttcaca tataaagggg ttctttgcag agaaccctta ccaaggctgg
      781 aagctaacgt aaaggggatt aagtatgctg aacgacttct cggagagata aaaaaccaaa
      841 tcagtgataa ctcattcgat ttttcaaaat actttccaaa ctccaggaag ttggcattat
      901 ttggaatggt aaagaagaat aaaagtgtga gagattacct ggaagagtat ttggaaattt
      961 gcaaaaacag aaagttatcg ccttcaacaa taaatggata tgaaaaatgt gtatctgcat
     1021 tatcggaact acataagcta catgttgttg acctgacacc agcaatatta aaaaaatgga
     1081 tttcaaaaag aacaacaaaa cttaagacca taagaaacaa tctgtcattt ttaagaagcg
     1141 cagtagatga agcggtcact gacggactta taacgataaa tcctgtaaaa ttggttagtg
     1201 caagtagata ccatgcagtt aaccatacag ccaatgctga tgattatgaa gtggatccct
     1261 tcacgccagc agaggcaaca gctatctaca atgcctgtat ctatcaggaa tggagaaatc
     1321 ttttttgttt cgcattcaat acaggccttc gaagctcaga attatgtgca ttgcgttggt
     1381 ctgatattga ttttattggg cataccgttc acgtacaggt tgcttctgtc gttggtgtga
     1441 taaaaggaac caaaacaaaa gctggtacca gaaaagttga attaaacaat gaagccataa
     1501 tggcattgca ggcccagaaa tcatttacga tgatgagaag tgattttgta ttcagtgatc
     1561 caaaaacacg taagccgtgg gctaattcag ctgcaattcg taagaaagcc tggatcccga
     1621 cccttaaaaa agctggagtt cgttaccgca atccatatca gaccagacat acattcgcta
     1681 cacggcatat cagccagggg gcaaatttat tctggcttgc cggtcaaatg ggtcacaaag
     1741 gaccagaaat gctgtttaga cactatggtt catatttatc agaatatgat ggcaagactg
     1801 cgatctcaga agtaatctag ctgcttaaat atttcaaaat atgtactaac gtaggacaca
     1861 gaatagactc tgacttacac ctgaaataca taaaaacata caaaaacaat aagaaatctt
     1921 ttgtttttca tatcgttatg taatttgggt gtttgggttc aactcccgcc agctccacca
     1981 atcatgattg gacggtgtaa ggactacacc aacaaaaaca ggaagttata agtctcagca
     2041 ggacaccgac cagacggtga ggagacaaaa aaggatacgc aacggagtcg cgactcccta
     2101 tgacattaaa gcccgctgat gcgggctttt ttattggtcc tgacccgccc tgaatagaat
     2161 tcatccaatt acagtccaga catatacagt atccctcagc tcagtatcag cttctcgaca
     2221 aatggtcact gttatttacc cgttgccaga aagagaataa tttgccccca tagccagcct
     2281 tcccggaatc tgtatattca ttaatattgt tatcgattcc atcaagtaac ataggttgga
     2341 tgcgattgat cttactcaca ttagtcgaca cggaactcag taagttgaac tctgaaaaag
     2401 cagtttaaat agcatttaaa caaatgccac cagttcgaaa aa
//
LOCUS       DAZKHK010000301         2431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKHK010000301 DAZKHK010000000
VERSION     DAZKHK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.303"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..75
                     /locus_tag="ACHOS5_005270"
     CDS             <1..75
                     /locus_tag="ACHOS5_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779912.1"
                     /translation="DYSKNGAGIENYNFITTAGLKYTF"
     gene            307..975
                     /locus_tag="ACHOS5_005271"
     CDS             307..975
                     /locus_tag="ACHOS5_005271"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9779913.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(1030..1404)
                     /locus_tag="ACHOS5_005272"
                     /pseudo
     CDS             complement(1030..1404)
                     /locus_tag="ACHOS5_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1417..1998
                     /locus_tag="ACHOS5_005273"
                     /pseudo
     CDS             1417..1998
                     /locus_tag="ACHOS5_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          755 a          552 c          501 g          623 t
ORIGIN      
        1 gactacagca aaaatggtgc aggcatagaa aactataact tcatcactac tgctggtctt
       61 aagtacacct tttaacaacg ttaaatagat tttctccccg gtggtattgt atagccccgg
      121 ggaatttgtt taccgttaaa agtaaacaat atcccctaat cttgtaagca tttaacctgc
      181 gtcctggcgt gtaacctctt accgcaacac aagactcaat atattcaaat tcgttatgcc
      241 gtggcatcat ttaacccctt gtaattcatc gctataattg atttaattca caaataaaac
      301 tataacatgg tgaaactaat gaaaaaaaac acagatgatg gggctaaaat ttacacacca
      361 cttaccctaa agctttatga ctggtgggtt ttgggtgtat caaatcggct tgcatgggga
      421 tgtcctacaa aggaacacct tcttccacac tttctggaac atttaggtaa caaccatctg
      481 gatattggcg ttggaactgg gttttacctt actcacgtac ctgagagtag tctgatatct
      541 ttaatggatt tgaacgaagc tagcctgaac gcggcatcga caagggctgg ggaatcaaaa
      601 attaaacata aaattagcca tgatgttttt gatccttatc ccgcggcgtt acatggtcaa
      661 tttgattcca tttccatgtt ttaccttctt cactgcctgc ctggaaatat atctacaaaa
      721 agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgacg atggaactct atacggagcc
      781 acaattcttg gtgacggcgt tgtgcacaat agcttcggtc aaaaactgat gcgcatttac
      841 aatcagaaag gcatcttttc aaacacaaaa gattccgaag aaggcttaac acatatactc
      901 tcagagcatt tcgagaatgt taaaaccaag gttaaaggta ctgtagtaat gttttccgct
      961 tcagggaaaa aatagcatcc agccgcagca cgttcttgcg cacgacgttc tgcggcataa
     1021 tcccaatgat tactctctga cagggttcac aggccactca atatcagggg cagttgatgt
     1081 atcaactcgg ttcagcaaca cccgatacat cttacaggct tccagcaacg aggtttcttc
     1141 ctccgtatcc tttatccatg atgtgccgtt ccacttctga aactcccctg ccggggataa
     1201 ccaggtgaca ttttccggta acggaccgag ttcagaaata aataatgcgt cccctgatgc
     1261 cacgtcataa accgttttcc cccgatggtc ttcaacgaga tgccacgatg cctcatcaca
     1321 gttgaaaaca accacgaagc cagccggaat atctggcggt gcaatatcag tactgtttgc
     1381 aggcaaacct gtatgaggtg gaatggcaca agagacttac aaaagaatca ggccaaacgc
     1441 ctgattgatg agagccccga accactgaaa tctgtcgtca aatttgcgct ggtaactgtt
     1501 ctgagaaagt cgaacatcat aaatctggag tggcaacaga ttgacatgca gcgacaagtt
     1561 gccagggtga atccagaaga tagcaaatca aatcgcgcca ttggtgtggc gctgaacgat
     1621 accgcctgat caaacaggca agcatcacaa atgtgggtgt acataccagg gcggctaagc
     1681 gagcagatgg aacatcaatg cctgcgctca ggaagatgcg cattgacagc aagacagcat
     1741 ggctatcagc ttgtcgtcgt gcaggaattg aaaatttccg tttccatgac ctcagacata
     1801 cttgggcaag caggttgatt ccgtcaggtg ccccattatc agtgcttcag gaaatgggcg
     1861 gatgggagtc caaagaaatg gttcgtagat atgctcacct tgcgcctaat catttgactg
     1921 agcacgcgag gaaaatagac gacatttttg gtgataatgt cccactctgg aattatagaa
     1981 gaaataaaga aggcgtaact gattgaattt taatggcgcg ccctgcagga ttcgaacctg
     2041 cggcccacga cttagaagtt cctagaacga cattttaagt caacaaatta ccgcgccatc
     2101 tctgcgctca cacgtcccac tacctcaaaa catgtaaagc cttgcaagcc attgtgaggc
     2161 cttatgtgtc tcagttttgt ccctcttttt tgtactaaaa aacatagcaa ttgaggataa
     2221 acctcatgct attttcgctt atatgccttt aaaggcatgg cgcttaaata gataaaaaca
     2281 ccacaaaagc ataaaaaacc acacagtaaa accgcaacat aaaacaataa cagataatta
     2341 aaccaaaaac agatagcgca ttgtgataat cattcaatac taaacaaaat ataaacagtg
     2401 gagcaatatg tgattgactc attaagttag a
//
LOCUS       DAZKHK010000302         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKHK010000302 DAZKHK010000000
VERSION     DAZKHK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.304"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..452
                     /locus_tag="ACHOS5_005274"
     CDS             72..452
                     /locus_tag="ACHOS5_005274"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9779914.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            449..796
                     /gene="tnpB"
                     /locus_tag="ACHOS5_005275"
     CDS             449..796
                     /gene="tnpB"
                     /locus_tag="ACHOS5_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9779915.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            846..2384
                     /locus_tag="ACHOS5_005276"
     CDS             846..2384
                     /locus_tag="ACHOS5_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP9779916.1"
                     /translation="MNDISSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGRITEAACWAHA
                     RRKIHDVHVRIPSALTEAALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          554 a          667 c          698 g          481 t
ORIGIN      
        1 tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta gtgggaacca
       61 aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc cctaattatc
      121 ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata tccatctcaa
      181 aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga caacaatggc
      241 gcgagggaaa gctgctatta ccttcttcag agagccccca gctacttcct gtgactctcg
      301 atgcagctgc cgaacagcca gaatcgctcg cagaggaccc ggaaaccctc agtatcagct
      361 gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc gaaaagctcc
      421 tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga ccaaaatttg
      481 gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg cgaaagtaca
      541 gacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg gccgcagcgg
      601 cagtcaggtt aaactgctgt ggtccaccgg tgacgggctg tgcctcctga ccaaacggct
      661 ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc ttacgcaggc
      721 gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc tgctgacctc
      781 cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg gtaaaatacg
      841 ggcttatgaa cgacatctct tctgacgaca tcctcctgct gaaacagcgc ctggccgaac
      901 aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa ccgggagcgc gaaatagacc
      961 atctgcaggc gcagctggat aaactccgcc ggatgaactt cggcagtcgt tccgaaaaag
     1021 tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcagaaa gagagcgata
     1081 cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag acccgcaccc
     1141 gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct gcagcgccgt
     1201 gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccgcc gaacagctgg
     1261 agttgatgcg tagcgccttc cgggttatcc ggacggtacg ggaaaaacat gcctgtactc
     1321 agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg ggtatcgccg
     1381 gaccggggct gctggcccgc gtgctgacct cgaagtatgc agagcacacc ccgctgtatc
     1441 gccagtcaga aatatacggc cggcaaggtg tggagctgag ccgttcactg ctgtcgggct
     1501 gggtggatgc atgctgccgg ctgctgtctc cgctggaaga ggcgcttcat ggctatgtca
     1561 tgactgacgg caaactccat gccgatgata ccccggtcca ggtactgctg ccgggtaata
     1621 agaagacgaa gaccgggcgg ttgtgggcgt atgttcgtga tgaccgcaat gccgggtcag
     1681 cgttggcacc tgcagtgtgg ttcgcttaca gcccggacag aaaaggcatc catccgcaga
     1741 ctcatcttgc ttgcttcagc ggtgtgctgc aagcggatgc gtacgccggg ttcaacgagc
     1801 tgtatcgcaa tggtcggata acggaagctg cctgctgggc tcatgcccgc cgaaagatcc
     1861 acgatgtgca cgtccgcatc ccgtcagcac tgacggaagc agccctggag cagatcggtc
     1921 agttgtacgc catagaggcg gatataaggg gaatgccggc agagcagcgg cttgctgaac
     1981 gtcagcgaaa aacgaaaccg ctgttgaaat ccctggaaag ctggttgcgt gaaaagatga
     2041 agaccctgtc gcgacactca gagttggcga aggcgttcgc gtacgcactt aaccagtggc
     2101 cggcactgac gtactatgcg aacgatggct gggtggaaat cgacaacaac atcgctgaaa
     2161 atgccctgcg ggcggtcagt ctgggtcgta aaaacttcct gttcttcggc tctgaccatg
     2221 gtggtgagcg gggagcgcta ctgtacagcc tgatcgggac gtgcaaactg aatgacgtgg
     2281 atccagaaag ctaccttcgc catgtgcttg gcgtcatagc agactggccg gtcaaccggg
     2341 tcagcgaact gcttccgtgg cgcatagcac tgccagctga ataacacatc cccgtcaata
//
LOCUS       DAZKHK010000303         2391 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKHK010000303 DAZKHK010000000
VERSION     DAZKHK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.305"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1250)
                     /locus_tag="ACHOS5_005277"
     CDS             complement(<1..1250)
                     /locus_tag="ACHOS5_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9779917.1"
                     /translation="MINKNKVTERSGIHDTVKSLSENLRKYIEAQYHIRDEGLIAERR
                     ALLQQNETIAQAPYIEATPIYEPGAPYSELPIPEAASNVLTQLSELGIGLYQRPYKHQ
                     SQALESFLGEDASDLVIATGTGSGKTESFLMPIIGKLAIESSERPKSASLPGCRAILL
                     YPMNALVNDQLARIRRLFGDSEASKILRSGRCAPVRFGAYTGRTPYPGRRSSRRDELF
                     IKPLFDEFYNKLANNAPVRAELNRIGRWPSKDLDAFYGQSASQAKTYVSGKKTGKQFV
                     LNNWGERLITQPEDRELMTRHEMQNRCPELLITNYSMLEYMLMRPIERNIFEQTKEWL
                     KADEMNELILVLDEAHMYRGAGGAEVALLIRRLCARLDIPRERMRCILTSASLGSIED
                     GERFAQDLTGLSPTSSRKFRIIEGT"
     gene            complement(1247..2290)
                     /gene="druD"
                     /locus_tag="ACHOS5_005278"
     CDS             complement(1247..2290)
                     /gene="druD"
                     /locus_tag="ACHOS5_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruD"
                     /protein_id="HFP9779918.1"
                     /translation="MEWRAVSRDKALDMLSTALNCRFDDEGLRISAVSECLRSVLYQY
                     SISETEEARQTVTSLRLTSAVRRKLVPLWPDIADIDNAIHPGIMSILNSLAELGDMIK
                     LEGGNWLTAPPHAVRIDNKMAVFFGGEPSCTFSTGVVAKSAGRVRLVEEKVCTGSVEI
                     WDANEWIGAPAEGNEEWSSRLLSGTISGFIDAPSNMSETTAYVRGKWLHLSELSFNKK
                     QIYLCRMSVDNHFSYYLGEIEAGRLCRMNSLESSDDVRRLRFFLDTKDNCPLKIRIKI
                     SNGLARLRLTRRLPRRETKVLLLGWRESGFENEHSGITHHVFPEEILPIVRSAFEGLG
                     IIWINEFTRRNEI"
BASE COUNT          660 a          552 c          452 g          727 t
ORIGIN      
        1 gtaccctcaa taattcgaaa tttcctcgaa gaggttggtg ataagccagt taagtcttgg
       61 gcaaaacgtt ctccatcctc aatggaccct agactagcac tggtaaggat gcagcgcata
      121 cgttcccggg gaatatccaa ccgagcacag aggcgacgta ttaaaagggc tacctctgct
      181 ccccctgctc ctctatacat atgcgcttca tcaagcacta agataagctc attcatctca
      241 tcagctttga gccattcctt agtctgctca aaaatattac gctcgatagg tcgcatcagc
      301 atatactcaa gcatggagta gttcgttatc agtaattctg gacagcgatt ctgcatttca
      361 tgccgggtca ttagctcacg atcctcaggc tgggtaatta gcctctcccc ccaattgttc
      421 aaaacaaatt gcttacccgt ttttttgcct gagacgtagg ttttagcctg agatgcgctt
      481 tgcccataaa aagcatcaag atctttactt ggccagcgac caatgcggtt cagttccgca
      541 cgtacagggg cgttatttgc gagtttattg taaaactcat cgaaaagggg tttgataaaa
      601 agctcgtctc gtctagagct acgacgacca gggtaaggcg ttcttcccgt ataagcgcca
      661 aagcgtacag gggcacatct tccagatctc agtattttag aggcttcaga atcaccaaaa
      721 agacgtctga tacgagcaag ttgatcgtta actaatgcat tcattggata taataaaatt
      781 gctctacaac ctggaaggga tgcagattta ggtctctcgg aagattcaat cgccaattta
      841 ccaataattg gcattagaaa gctttcagtc ttaccggagc ctgtacctgt tgcaatgacc
      901 agatcagaag cgtcttcgcc aagaaatgac tcaagtgcct gtgattggtg tttatagggg
      961 cgttgataga ggccaattcc aagttctgat agttgagtta gcacattact tgctgcttcg
     1021 ggaataggta attcactgta tggcgcacca ggttcataaa ttggggttgc ttctatataa
     1081 ggagcttgag caatagtttc attttgctgt aaaagcgctc gtcgctcagc aattaaccct
     1141 tcatcccgga tatgatattg tgcctcaatg tattttctca gattctcact aaggcttttc
     1201 acggtatcat gtatacctga acgttcagtt actttatttt tattaatcat atttcattcc
     1261 gtcgcgtgaa ttcgttaatc caaataatac caagcccttc aaaagcgcta cgcactatgg
     1321 gtaatatttc ctcggggaat acatggtgtg ttattcctga atgttcattt tcaaaacctg
     1381 attctctcca gcctagcagg agtaccttcg tttctcgtct tggtaatctt ctggttaatc
     1441 ttaatcttgc tagcccatta gatattttga tacggatctt tagcggacaa ttatcttttg
     1501 tatcgagaaa aaaacgtaat cttctgacat catcagacga ttctaacgaa ttcattctac
     1561 ataagcgtcc agcttcaatt tctcctaaat aataggaaaa gtgattatca acggacattc
     1621 tgcataagta gatttgcttt ttattaaaag aaagttctga caaatggagc cattttcccc
     1681 gcacatatgc agtcgtttca ctcatattgc taggcgcatc gataaagccg gaaatagttc
     1741 cagatagtag tctggatgac cattcttcat tgccttctgc tggggcacca atccactcat
     1801 ttgcatccca gatttcaaca cttccagtac acactttttc ttcaaccaag cgaactcttc
     1861 cagcagattt agctaccacg cccgttgaaa atgtacagga aggctctcca ccaaaaaaaa
     1921 cagccatctt attgtcaatt cgtactgcat gtgggggagc tgtgagccaa ttaccacctt
     1981 ctaacttaat catgtcaccc aattcagcca agctgttcaa tatagacata atgcccggat
     2041 gtatagcatt atcaatatca gcaatgtctg gccataaagg taccaatttt cgccttactg
     2101 cactagtgag tcgaagcgag gttacagttt gcctagcttc ttctgtttca gatatagaat
     2161 attgatataa tacgctcctt aagcattctg aaactgctga aattctcaac ccttcatcat
     2221 caaatcgaca atttaatgca gttgataaca tatccagtgc tttgtctcgt gatactgctc
     2281 tccactccat taaccacctc ctcaagaaga tatttttgca ctataatttt ttaattcacg
     2341 attggcaata tcttctatct tttggacggg gccaccttta atttttgccc a
//
LOCUS       DAZKHK010000304         2390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKHK010000304 DAZKHK010000000
VERSION     DAZKHK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.306"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2043
                     /locus_tag="ACHOS5_005279"
     CDS             <1..2043
                     /locus_tag="ACHOS5_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779919.1"
                     /translation="TSKTDVLPTVSISVETTSTDVNGDGFTGIASVNGTVMDVPATIE
                     DKDDSTGLVYTVSLNHVTTTDVTVTVTLGSGAGHSDAADYSDIGGAQHNGKIGLHGDT
                     GKVTYDGATTVTIVIPAGSKSVSFIVDPTLEANQDAFNAEGMEKVVATITGTSDNVTA
                     ATDIVDNAGASATGVIYDGNAISLRNLDGDFTLKYSLSSSIAEKGDFGYTIGANSGEN
                     DPMVTTDYNDTVYVGYYQSGKETTSYSNVANSQDNGPDGTKTDGNQSITTVDLGAGDD
                     LMVIRGNMLANTRVYTGEGNDTFTMDGMNTALRVMYAGSYIFTESGDDIVTIKRTGVT
                     NAGQIYLGSGSDTFIQGDATDNNDTTLSGLLDLGSGTKDISNMPKEYLSVYQDGSNLS
                     LGNDNNIDTATDVNTVTIYGSVSGEILGGYGSDNITVTKNLTGNISVGDNADTLTAGW
                     IYGGSTVSMGDGNDTVTITDGAYNTTISLGAGDDVFDATSGVMGDSAYATVVNGEDGN
                     DTFKLGTIAKNLTIDAGAGDDIVVLTKDYDSTSSGNQGYINGGEGSDTLVLTGTISVN
                     LAAGKNEGIAGFEKVDMTVGSDLKADNAAQLVKLTASDVLGINDNSTIYISGDANDKV
                     DLGADGAGSLGTFTATATTVKATALDGIEHTYTLYSSVSGANVYIDNNIIDANGVI"
     gene            2164..>2390
                     /locus_tag="ACHOS5_005280"
     CDS             2164..>2390
                     /locus_tag="ACHOS5_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFP9779920.1"
                     /translation="MNIMIVTHNKYLELGLKKLLSRHSITIGADFFIPDNREHIINNN
                     IFVILCDKKNSMLMNYIFNGYRFYLLPVESI"
BASE COUNT          719 a          384 c          560 g          727 t
ORIGIN      
        1 acctcaaaaa ctgatgtgtt accaacagtg agtattagcg ttgagacaac atcaacagat
       61 gtcaatggtg atggttttac tggtatagct agtgtcaatg ggaccgtaat ggatgttccc
      121 gcaactattg aagataagga tgattccact ggactggttt atacggtttc tctgaatcat
      181 gtcactacaa cagatgtaac agttacagtt acattgggta gcggagctgg gcattctgat
      241 gctgctgact attctgatat tggtggagca cagcataacg ggaaaattgg ccttcatggt
      301 gatactggaa aagtaaccta tgatggtgca acaaccgtaa caattgttat tcctgccggt
      361 tcaaaaagtg tttcatttat agttgatcca acgcttgagg ctaaccaaga tgcctttaat
      421 gccgagggta tggaaaaagt tgttgcgacc attacgggaa catctgataa cgttacggca
      481 gctactgaca tcgttgataa tgcgggagca tccgcaacag gtgttattta tgatggtaat
      541 gccatttcat tacgtaatct ggacggtgat tttacactga aatattcact atcatcttct
      601 atcgccgaaa aaggggactt tgggtatacc attggcgcga atagtggtga aaatgatcca
      661 atggtaacaa cagattacaa tgatacggtt tatgttggtt attaccagag tggtaaagaa
      721 acaacatcgt atagtaatgt tgctaactca caggataatg ggccagacgg aacaaaaacc
      781 gatggcaatc aaagcattac gactgttgat ttaggtgctg gtgatgatct aatggtcatt
      841 cgtggtaaca tgctggctaa tacaagagta tatactggtg agggtaacga tacttttact
      901 atggatggta tgaatactgc attacgtgtt atgtatgcgg gtagttatat ctttactgag
      961 tctggcgatg atattgttac gatcaaacgt actggtgtaa caaatgccgg acagatttat
     1021 cttggttcag gtagtgatac atttatccag ggggatgcta ctgacaataa cgatacaaca
     1081 ttatctggtt tattggatct tggtagtggt acaaaagata tatctaatat gcctaaggaa
     1141 tatctttctg tttatcagga tggcagtaat ttaagcttgg gtaacgacaa caatattgac
     1201 actgccacag atgttaatac cgtcactatt tatggctctg tgtctggtga aattttaggc
     1261 ggatacggca gtgataatat tacggtaact aaaaatctga ccggtaatat ttctgtcggt
     1321 gacaatgcgg ataccttaac ggccggatgg atttatggtg gcagtactgt ctctatgggg
     1381 gacggcaatg atactgttac catcacagat ggtgcatata acacgactat ttcactgggg
     1441 gcgggagatg atgtatttga tgcaacttct ggtgtaatgg gggattctgc ttatgccact
     1501 gtcgttaatg gtgaagacgg caatgatacc tttaaattag gcacaattgc caaaaacctg
     1561 accattgatg ccggtgccgg tgatgatatt gttgttctga caaaagacta tgactccacc
     1621 tcctccggta atcagggcta tattaacggc ggggaaggca gtgataccct ggttctgaca
     1681 ggtaccattt ctgtcaatct agcggccggt aaaaatgaag gtattgccgg cttcgagaaa
     1741 gtggatatga ctgttggtag cgatctgaaa gctgacaatg cagctcagtt agtcaagtta
     1801 acggcatcgg atgtattggg tataaatgac aattcaacga tttatatttc cggtgatgcc
     1861 aatgacaaag ttgatttggg ggctgacgga gcgggaagtc tgggtacatt tacggcaacg
     1921 gcaacaactg ttaaagcaac tgcgttagat ggtattgagc atacttatac gttatacagc
     1981 agtgtaagtg gcgcaaatgt gtatattgat aacaatatta ttgatgcgaa tggtgtaatt
     2041 taatagttgt cttattcatg tgggagggaa acttcccaca tttttgagtg ttatagaaac
     2101 aacttaataa tttgcaaatg agtcaatatg acatttatat acttcttgta gaaggcataa
     2161 tgtatgaata taatgattgt tacgcacaat aaatatcttg agttaggttt gaaaaaatta
     2221 ttgagcaggc attctattac tattggtgct gattttttta taccagataa tcgtgagcat
     2281 ataataaaca ataatatttt tgtcattctc tgtgataaaa aaaacagtat gttaatgaat
     2341 tatattttta acggttatag attttactta ttaccagtag aatcaataag
//
LOCUS       DAZKHK010000305         2336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKHK010000305 DAZKHK010000000
VERSION     DAZKHK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.307"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(68..280)
                     /locus_tag="ACHOS5_005281"
                     /pseudo
     CDS             complement(68..280)
                     /locus_tag="ACHOS5_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            430..1098
                     /locus_tag="ACHOS5_005282"
     CDS             430..1098
                     /locus_tag="ACHOS5_005282"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9779921.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1043..1180)
                     /locus_tag="ACHOS5_005283"
     CDS             complement(1043..1180)
                     /locus_tag="ACHOS5_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFP9779922.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1153..1737)
                     /locus_tag="ACHOS5_005284"
     CDS             complement(1153..1737)
                     /locus_tag="ACHOS5_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9779923.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEEIKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1737..>2336)
                     /locus_tag="ACHOS5_005285"
     CDS             complement(1737..>2336)
                     /locus_tag="ACHOS5_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9779924.1"
                     /translation="YPKLAAAYPSGVIPDLRGWTIKGKPASGRAVLSQEQDGIKSHTH
                     SASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFK
                     DTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIG
                     AHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          635 a          556 c          485 g          660 t
ORIGIN      
        1 gaaaactgat attactttaa tcaaaagttg acgtgccaga ttcgaagtaa atttataaaa
       61 tatcaaatca atatacattt tgcaccttcg agatagtaat gactgttact gtaacatgag
      121 ttacaaacag cttcataccc agagctactg atgtatcaac tcgattcaac aatacccgat
      181 atttattcca tgcctccagc aacgatcttt cttcctccgt tgcgatttcc agatctacag
      241 catcctgcag tggcgcaata tactcactga attcctggat gtagaactgt gtggtgacgg
      301 tcttccagcc attcggctcc tgttgtatcg aagcatacca ggctatttca atatcgctat
      361 gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat tcacaaataa
      421 aactataaca tggtgaaatt aatgaaaaaa aacacagatg atggggctaa aatttacaca
      481 ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg gcttgcatgg
      541 ggatgtccta caaaggaaca ccttcttcca cactttctgg aacatttagg taacaaccat
      601 ctggatattg gtgttggaac tgggttttac cttactcacg tacctgagag tagtctgata
      661 tctttaatgg atttgaacga agctagcctg aacgcggcat ctacaagggc tggggaatca
      721 aaaattaaac ataaaattag ccatgatgtt tttgaacctt atcccgcggc gttacatggt
      781 caatttgatt ccatttccat gttttacctt cttcactgcc tgcctggaaa tatatctaca
      841 aaaagctgtg taatacgcaa tgcggcgcag gccttaactg acgatggaac tctatacgga
      901 gccacaattc ttggcgatgg agttgtgcac aatagcttcg gtcaaaaact gatgcgcatt
      961 tacaatcaga aaggcatctt ttcaaacaca aaagattccg aagaaggctt aacacatata
     1021 ctctcagagc atttcgagaa tgttaaaacc aaggttcaag gtactgtagt aatgttttcc
     1081 gcttcaggga aaaaatagca tccaaccgca gcacgttctt gcttaagacg tgctgcggca
     1141 taatcccaat gattactccc tgacagggtt cgtaggccac tcaatatcag gtgcagttga
     1201 tgtatcaaca cggttcagca acacccgata ctttttccag gcttccagca atgaggtttc
     1261 ttcctccgtt gcgatttcca gatctacagc atcctgaagt ggcgcaatat gctcactggc
     1321 tacctgcatc aggctgtttt ttatttcttc cgcctctcgg atccggaaca gtttttctgc
     1381 ttctgcatct ttcacccagg ctgtgccgtt ccacttctga aactcccctt ccggcgataa
     1441 ccaggtaaca ttttccggta acggaccgag ttcagaaata aataactcgt cccctgacgc
     1501 tacgtcataa accgttttac cccgatggtc ttcaacgaga tgccacgatg cctcatcact
     1561 gttgaaaaca gccacaaagc cagcaggaat atctggtggt gcaatatcgg tactgtttgc
     1621 tggcagacct gtatgaggcg gaatatatgc atcaccttca ccaataaatt cattagttcc
     1681 ggccagcaga ttataaattt ttatggtccg tgcttgttca ctcattctga atgccattat
     1741 gcaagcctca caatatagtt aaatgcgatg tttttgacgg tgttttccgc gttaccagca
     1801 gcgttaacgg tgatggtgtg tccatgtgaa ccaatcgcaa cggagtgcgt atgagcacca
     1861 ataccgacag tatgcgcgtg tgcacctgca gatgctgctg tgccggacag tgaatggcta
     1921 tgattaccat ctgtactggt attcgctaac caccccgtag acatacctac tgagccttgt
     1981 acaccccagg tattttgacc tgagcttgta taaccatatt gataagtatc tttaaaaaca
     2041 ctggggttaa atcgacggcc atctctatgg ctgtgattac cagctgcatt cgtgctgcca
     2101 cttaaactat gggtatgcgc accagtgtta ttcgtggatt tagtgccgta atcaaacgac
     2161 gatgtggttt tcgtccccaa atccgtactg gatgcgctgg cgctgtgggt atgcgattta
     2221 atgccatcct gttcctgaga caatacggca cgaccactgg cgggcttgcc cttaatcgtc
     2281 cagccacgca aatcagggat cacgcctgac ggataagcgg cagcaagttt cgggta
//
LOCUS       DAZKHK010000306         2308 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKHK010000306 DAZKHK010000000
VERSION     DAZKHK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.308"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..300)
                     /locus_tag="ACHOS5_005286"
     CDS             complement(<1..300)
                     /locus_tag="ACHOS5_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779925.1"
                     /translation="MEVINKETDSIQTKSNSAVLSYIYGQSGLTIDLNKCFVRLFNNA
                     SGINALYGYNQIREINYTLPNTDFGEAEICILTSGSNNALWKFTVKSEDNERVCER"
     gene            670..1200
                     /locus_tag="ACHOS5_005287"
     CDS             670..1200
                     /locus_tag="ACHOS5_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9779926.1"
                     /translation="MKKILLVAGAALALAGCGEKGDFEKAINAKIGQDKYCFSLDNNN
                     TSFPIRLAKPRLDSTGTGTNSVILDGFVEQGLMVFDQGYDSNVLGITDEGIKAKVWST
                     TDGACVGRRAVDEIKEWTEPGNGGQKVVRVSYTWKLVDVPGWIDKKAFANVKGMNEPA
                     DGAINLVKTSNGWKAN"
     gene            1211..1486
                     /locus_tag="ACHOS5_005288"
     CDS             1211..1486
                     /locus_tag="ACHOS5_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779927.1"
                     /translation="MENNTIKCPFCFKESQRGVHVCTGCLATVLYGTYPGWYAAVVLL
                     LTFGLSIIIGMSTGMAGATISLPIIAAIGFVAGKAIFSDNVVFRRRM"
     gene            1672..>2308
                     /locus_tag="ACHOS5_005289"
     CDS             1672..>2308
                     /locus_tag="ACHOS5_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFP9779928.1"
                     /translation="MIIFEIVSYKRTLKKMNTIYHYCSPESFFSIIQNQRLWLSSMDH
                     MNDYMEKKWFYSTLKKYLYKNLDANCVDQFIAHLDDNISVGTPFACCLSKSGDILSQW
                     RAYAKDGFGVSIGFDREKLDVYDGIIGNHLDPKHRLTLSDISYMDINEIECLAERILS
                     RYSFIKKYYMNEIISTSKFNRYDKCILELISNIIHLNTTTKNPAFEEEKEVR"
BASE COUNT          710 a          408 c          499 g          691 t
ORIGIN      
        1 tctctcacaa actctttcat tgtcttccga cttaacagta aacttccata aagcattgtt
       61 gcttccagat gtgagaatac agatctctgc ctcaccgaaa tcagtgtttg gtaatgtgta
      121 attgatttcc cggatttggt tatagccata taaggcatta atgccagatg cattattgaa
      181 tagccgcaca aaacatttat tcagatcaat ggtgagtcca gactgtccat agatatatga
      241 taaaacagca ctgttagact ttgtttgaat gctgtcggtt tccttgttga tgacttccat
      301 actcacctca ccagatccta ctgaacgacc tacgcgaagc atatcatttt gtgaacagat
      361 ctcaataaaa tgatcgatat aaaagatcga taattgatta ttgatagatt ttgacgattg
      421 aacaggtggt atcgatcggt gattaattgt tacaatcgat cgaatatcta tttatgatag
      481 ataaaaacta tcataagatt gtttttgatc gttttaaacg atatgacgag acgctaaaag
      541 aattgaatcc tatcaattgg ttaagcggtg gttttgccat gtgatatcag ccggatcccg
      601 caaccactct agccctgacc ggattaggtc aaaaatttag gcactttgat tatcaggatg
      661 gataagcaga tgaagaagat tttactcgtt gctggcgcag ctctggcact ggcgggatgt
      721 ggtgagaaag gtgattttga gaaagcgatt aatgccaaga ttgggcagga caaatactgc
      781 ttctcgctgg ataacaataa caccagcttc cccatccgtc ttgccaagcc aaggctcgat
      841 tcgactggca cagggacgaa ttcagtgatc ctcgatggct ttgttgagca gggcctgatg
      901 gtctttgatc agggctatga ctcgaacgta ttagggataa cagacgaagg aataaaagct
      961 aaagtctgga gcacaacaga tggtgcttgt gtaggtcgtc gtgccgttga cgagattaag
     1021 gaatggacgg aaccgggtaa cggcggccag aaggttgttc gcgtgtctta tacatggaag
     1081 ttggttgacg ttccgggatg gattgacaaa aaagcgttcg ccaacgtcaa aggaatgaat
     1141 gaacctgctg atggtgcaat caatctcgtt aaaaccagta atggctggaa agccaattaa
     1201 ggaaaccaat atggaaaaca ataccatcaa atgcccattc tgtttcaaag agagccagcg
     1261 tggggttcac gtttgcacag gctgtttagc aacggtgtta tatggcactt atcccggctg
     1321 gtatgctgct gttgttttgt tgttgacttt tggtctatct attattattg gtatgtcaac
     1381 aggcatggcg ggggctacta tcagcctacc aatcattgca gctattggct ttgtcgcagg
     1441 taaggcaatt ttctctgata atgtagtgtt taggcgaagg atgtaatcaa gcgtaaaaca
     1501 aaaaccatat tcttgattta taggagtatg gtttatatct atataagagg attggataga
     1561 gtgcccttaa tcggccttga tctgagacct taataaatca ggttcgcatc ttatctgaca
     1621 aattgctccc tgctattaga aacagatgtg ctggtaatga aattgtgtgg tatgataatt
     1681 tttgaaattg tctcttataa aaggactttg aagaagatga atacaatcta tcattattgc
     1741 agcccggaat ctttcttcag tattattcag aatcagcgat tatggctaag ctctatggat
     1801 catatgaacg attacatgga gaaaaagtgg ttttactcga cacttaaaaa atatttatat
     1861 aaaaacttag atgctaactg cgttgaccag tttatagcac atttggatga caatatatct
     1921 gttggaacac catttgcttg ttgtctatct aaatctgggg atattttaag tcaatggcgt
     1981 gcgtatgcaa aagatggctt tggtgtatcc atcggattcg acagagaaaa actcgatgtc
     2041 tatgatggga ttataggtaa tcaccttgat cctaaacacc gactaactct tagcgatata
     2101 tcttatatgg atattaacga aattgagtgc ttagctgaaa ggatattatc aagatattca
     2161 ttcataaaaa aatactatat gaatgaaatc atatcgacat caaaatttaa ccgctacgac
     2221 aaatgtattc ttgaattaat ttccaatatt attcacttaa ataccacaac taaaaaccca
     2281 gcgtttgagg aggaaaaaga agtacgat
//
LOCUS       DAZKHK010000307         2291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKHK010000307 DAZKHK010000000
VERSION     DAZKHK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.309"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(94..1023)
                     /gene="metA"
                     /locus_tag="ACHOS5_005290"
     CDS             complement(94..1023)
                     /gene="metA"
                     /locus_tag="ACHOS5_005290"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP9779929.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1180..1626
                     /locus_tag="ACHOS5_005291"
     CDS             1180..1626
                     /locus_tag="ACHOS5_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9779930.1"
                     /translation="MVISIRRSRHEEGGGELVAIWCRSVDATHDFLSAEYRAELEELV
                     HSFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPE
                     LTTNVNEQNEQAVGFYKKVGFKVTERSEVDDLGKPYPLLNLAYVGA"
     gene            complement(1689..2072)
                     /locus_tag="ACHOS5_005292"
     CDS             complement(1689..2072)
                     /locus_tag="ACHOS5_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFP9779931.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMFKQK"
     gene            complement(2149..2264)
                     /gene="rrf"
                     /locus_tag="ACHOS5_005293"
     rRNA            complement(2149..2264)
                     /gene="rrf"
                     /locus_tag="ACHOS5_005293"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          585 a          525 c          606 g          575 t
ORIGIN      
        1 cacttgctga ggtgtgtttc ggaaataaaa aaggcacccg aaggtgcctg aggtaaggtg
       61 ctgaatcgct taacgatcga ctatcacaga agattaatcc agcgttggat tcatgtgccg
      121 tagatcatat ggcgtgatct ggtagacgta atagttgagc cagttggtaa acagcaaatt
      181 accgtgacta cgccagctcg ctcgcggtgt attttgcgga tcattgtgcg ggaaatagtt
      241 atacggtaca tccgggtcta gcccggcttc cacatcacgg aaatactcct gcgccagcgt
      301 ttgcgcatca tattcgggat ggcccgtcac aaaggcaatg cgcttatctt tgctggcaaa
      361 cagatatgca tccccttctt ccgtctctgc cagaatttcc agatcggtat aatcacgaat
      421 caacgctgcc ggaaagtcag catagcgcga atgcggtgcc aggaatgaat catcaaagcc
      481 acgcgtcaga agcgcatgag gatggagaat atgatgctcg taaacgccag agagtttgtc
      541 ggtgcgggtt tgcttaggaa taccatagag gatattgagc gcggcctgta cagcccagca
      601 gacaaacagc gtcgaggtga cgtgatcttt cgaccactcc agcacctgtt tgatctgcgg
      661 ccagtaagcg acatcattaa actccaccag gcccagcggc gcgccggtaa ctatcagacc
      721 gtcaaagttt tgttcctgaa tatcttcaaa gttacagtag aagttattca gatgctctgc
      781 gggcgtgttg cgcgattcac gggaatcgat gcgcaacagc tgaatatcga cctgcaaagg
      841 tgagtttgaa agcaggcgca gaaactgatt ttcagtttcg atcttcttcg gcatcaggtt
      901 aaggataagc acctttagcg gacgaatttc ctgaccagac gcgcgagaag ttgtcatcac
      961 aaagacgttt tcttcacgca agaaattgac ggcgggtagc tcgtccggca cacgaatcgg
     1021 cataacctga ttacctcact acatacgctt atacgtttag acatccagat agctgaagat
     1081 aaccagaaaa tttaccaatg tcgagcctgc atgttgaagg tgagaaagtt tcaaggaaaa
     1141 ggctacgtta gaatataaga atgacgaaaa ggagagaaaa tggttattag tattcgacgc
     1201 tcacggcatg aggagggggg gggggaactc gttgcgattt ggtgtcgttc tgtcgatgcc
     1261 actcacgatt ttttatcagc agagtatcgg gccgagctgg aagagctggt gcattccttt
     1321 ctgccggaag caccgttgtg ggtcgcggtt aatgagcggg atcagccggt tgggtttatg
     1381 ttgttaagtg ggcagcatat ggatgcgctg tttatcgatc ctgatgtgcg cggctgcggc
     1441 gtgggccgga tgctggtgaa gcatgcgctc tcaatggccc cggaactgac aaccaatgtt
     1501 aatgagcaaa atgagcaggc ggttgggttc tataagaagg tgggctttaa ggttacggaa
     1561 cgctctgagg tggacgattt ggggaaaccg tatccgttgc tgaatctggc atatgtgggg
     1621 gcgtaggcgg ttttttctca tcccggtggg ctgaacttta ggtttcgtac ccaccaatgg
     1681 tgacatgatt atttctgctt aaacattgcc atgaccgcac tatcactgag caccactgat
     1741 tgcgcatgga tgtggaactg gcgatatttc attaacgttg cccctgcgac cacttcatga
     1801 agaatgcgtt cctcaacctg tgacaaaccg ccctcattca tttccagcgc gctcacaaca
     1861 atatccatac gttttgctgg caaaaaagaa tgccaggttg aacgtgggtg gagttgcaga
     1921 acaagatctt gcagaacttt ttctgcaaca aagaaattgg cgattaacag gcgctggtta
     1981 ctgaaggcag catctcctga cacttcacgt ttgctttcaa gatcgcgaac agtaatttga
     2041 ttacgacgaa tttggatata cagaactgac atgagattcc cttcatcatg caaataattg
     2101 atatgcaaaa aaccccggcc ttattgaccg gggtttttct aatttgatgc ctggcagttc
     2161 cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag
     2221 ttcggcatgg ggtcaggtgg gaccaccgcg ctaaggccgc caggcaaatt ctgtttcatt
     2281 aacccgcttg t
//
LOCUS       DAZKHK010000308         2277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKHK010000308 DAZKHK010000000
VERSION     DAZKHK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.310"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            444..911
                     /locus_tag="ACHOS5_005294"
                     /pseudo
     CDS             444..911
                     /locus_tag="ACHOS5_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1590..>2277
                     /locus_tag="ACHOS5_005295"
     CDS             1590..>2277
                     /locus_tag="ACHOS5_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9779932.1"
                     /translation="MNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDESSQGYDVHG
                     DNWKSIEIGTTQEVKASPDKNINFDHNSPVAEGRNSIAIGTSAEAGKEMTDKMAGEAI
                     AIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENGYLASEAQ
                     GDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEANGDMATAVGFNALANAS
                     NALAVGSQSSA"
BASE COUNT          638 a          449 c          560 g          630 t
ORIGIN      
        1 atccactttg ttgtggttct gacagaagta aattaatttg aagtaaataa agcaaaatat
       61 cagaagaata tatcggggcg cacagtctat ctctgccttt gcttcatggg atgttctttg
      121 tatattttac ttgctgttta agtatgtata taaggagtgc attcattatg ccaatgaagc
      181 ttgtaaagaa gaatccccgg agctgggggc ggtttgggaa acggaattat tgtacgttag
      241 gagcattttt taatcgatgc ggataagtaa ctgattgatt catcgtgatc agctatcgcc
      301 tttaagttat tgcttttctc ttattgcatt taggttatct catgtcccta aaggtctgcc
      361 aaataccgta tagcgttgga gtacacccta ccgcaatagc gcaaccgtac aatattgtac
      421 gtttcccaaa cgcagcccta tcgggtgcac tgtacgctat cgagtctgag atccgcggca
      481 gtccggcaga agagcgactg gcggtcagaa aagccagaac ggtaccgcta atgcagtcgt
      541 tgtatgagtg gctccagggg cagatgagca cgctgtcgcg tcactcggat acggcgaaag
      601 cgttcaccta tctgctgaag taatgggacg ccctgaacga atactgccgc aatggctgga
      661 tggagatcga caataacctg tgtgaaaacg ccctccgggt agttgcactg gggcggtgta
      721 actacatgtt cttcggctct gatggaggag gcgagagtgc ggcagtgatg tacagcctga
      781 tcggtagctg caaacttaac ggaatcgagc cggaaacgtg gctacgccac gtgatcagag
      841 tcatcaacac ctggcctgcc aaccgcgtga aagagttgtt gccctggaac gtcactctat
      901 ctgtaaacta atttttaccc cacgtccttc acggtgcgct tacaagagta acgccgcttt
      961 ctgcgtaaaa aacaacagca gcaggtactt caaaacagtg atgcactgtc agttgcaaag
     1021 acgaatgtac agttaccttt gttaccagag gacaagaagt gccccgggta ttaccgtgaa
     1081 agaaatcagt gaacatttat tgaccataac cacattgctt ctccgtagac tgttgattta
     1141 aaagatttaa tttcctgtga aacaatcagg cctgtatgaa cctgattata ccacataatc
     1201 ttttaagtta tttaaattta aatctcaggc ttttctgtca gtaaaatcag aaaaattgca
     1261 ggatttcgtt ttttgctgca aagagaaatg tatctcattt ttactgcctt cgccagtgcg
     1321 tcagtcaatg gcaggtagtg tacctgcatt ctgctgtaac gcgtaatgtc gggtagttta
     1381 cttaaactta atatgtttta tgttcgcatt attataaatc actttattgc cttttgtctg
     1441 ctttttaatg gttcccgttt tttcacttct ggctttataa aaaatggatg ctgaagaaat
     1501 gaaggcagtc cgccttacat aatattcatg catgattttt atgctattcc gactgagtgg
     1561 aagaaaataa aggagaaaag aggatatata tgaacctcag aaaaaaacat atctgtatag
     1621 ccatatcact tgttcttaat ccgttccctg catcactggc agcacaaaac actaatgtag
     1681 ttgaagttga tgaaagcagt caggggtatg atgtgcatgg agataactgg aagagcattg
     1741 aaatagggac aacgcaggaa gttaaagcat cccccgataa aaatattaat ttcgatcata
     1801 attctcctgt tgcagaagga cgtaactcta tcgcgattgg taccagtgca gaggctggta
     1861 aagaaatgac ggataaaatg gcaggggaag ccatagctat tggtgccaag gcgaaagcaa
     1921 ctaaagaaca gtcaattgca ataggtggcg ataccaaagc taccggatgg ggggctattg
     1981 ttattggtgg ggatgattta actcctttgc atggtaagac ttatggtcgg gaaaaaatag
     2041 agaatggcta tctggcatcg gaagcgcagg gggatggcag tattgcgctg ggcagcagaa
     2101 cttttgctaa aggcgaattg tcagtatcgc ttggttcggt gagttctgct gaggggatgg
     2161 cctcgaatgc aattggagcc acggcggaag ctaacgggga tatggcgact gcggttggtt
     2221 ttaatgcgct ggctaatgcc agtaatgctc tggctgttgg tagtcagtcc tctgcca
//
LOCUS       DAZKHK010000309         2234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKHK010000309 DAZKHK010000000
VERSION     DAZKHK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.311"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1319)
                     /locus_tag="ACHOS5_005296"
     CDS             complement(<1..1319)
                     /locus_tag="ACHOS5_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP9779933.1"
                     /translation="MRRHNGRMAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVY
                     HWCDVGQWATLLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNK
                     HAERMAEIAALRPAGHEDGVAAGTCGGGEGKKRRYRKNDVSGLTAEMLDEWARKNLYA
                     YQLHCRENRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFRE
                     YIINFALQFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLH
                     EVASAMATHDKYRTTYFSTPSAKTHQAYLVWNGDDWRGDDPARRAVEFPKESAMRVGC
                     ECPDGIWRYIIRLEEAVAGGLSARVDIERIRNRYNPTTYAMLYGCEFVDSKDAVFKFS
                     ELVRCEVEMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAP"
     gene            1465..>2234
                     /locus_tag="ACHOS5_005297"
     CDS             1465..>2234
                     /locus_tag="ACHOS5_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9779934.1"
                     /translation="MPQPTLMTDWTCIATSGPTADGREIDPQWLIDAAETYSRNTYTA
                     MLWPCHENDTSYRQYTFNLGEVDALKVETREDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAELIPNFAKSGRDYLMGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTTPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAAAGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAE"
BASE COUNT          523 a          690 c          583 g          438 t
ORIGIN      
        1 ggagccacaa ttacaaacgt ggaattatcc ccggaccttg acgggtcaaa gcccgcccac
       61 acctcacgat tgccgaacgg acgtgctgcc gtgggatcgt agtcacccca cgtctccatc
      121 tccacctcgc acctgaccag ttcggagaac ttaaataccg catccttgct gtcaacaaac
      181 tcgcagccgt acagcatcgc ataggtggtc gggttgtagc ggttcctgat acgttcaata
      241 tcaacccgtg cagacaggcc acccgccacc gcctcttcca gccggataat gtagcgccag
      301 atcccgtcag ggcattcaca gcccacacgc atcgcgcttt ctttcggaaa ctcaacagca
      361 cgacgcgccg ggtcatcccc gcgccagtcg tcaccattcc agaccagata agcctggtgg
      421 gttttggcgc tgggtgtgga aaaatacgtc gtgcggtatt tgtcgtgggt cgccattgcc
      481 gaggccactt catgcagtcg cgtgaatttt gggatccaga acacctcatc gccgtaaagg
      541 tggccgttaa atccctgggc ggtactggca ttggtggaca ggaaacgcag cattgcacca
      601 ttgctcagac ggatatgctt acccgtgagc gtgatcccga actgcagggc gaagttaata
      661 atatattcac ggaagatttc cgcctgtacc cgtgacgcag aaaagaaaat ctggttatca
      721 ccggtcacca ccgcatcttc gaacgcctcc catgcaaaat accaggtcat gccgatctgg
      781 cggctcttga ggatgaaacg gcgctcacgg tgccggttct cgcggcagtg cagttggtag
      841 gcataaagat ttttacgcgc ccattcatcc agcatttcag cagtcagtcc ggaaacgtcg
      901 tttttccggt agcgccgctt tttcccttct cccccgccgc aggtccccgc agccacgccg
      961 tcctcatgcc ctgccggacg cagggcggca atttccgcca tcctttcagc gtgtttgttt
     1021 ttctgggcga tgatcttcac gtgctgcgcg ataagttcac gcagttcggc aagttcacac
     1081 tctgtcttgc cctcccgtcc ggtcagcaca tcaatacgcc gggcgatcac gtcctcaacg
     1141 gattcagcgg gcagcagggt agcccactgc cccacatcac accagtggta aaccgtgcgc
     1201 ggcggaatac cgagttccgc cgcaatatcg cgggggtgcc accgcttcag gtaaagcgat
     1261 cgcgccgcct ctttgatttc gtcagaatat ttagccatgc gcccattatg gcggcgcatc
     1321 cggcccgcat tcaggcagaa aatttcggct gtgttcggct attccgtgtt atccgaatgc
     1381 aaccgaatta atccagtagc cccctgccgt aaaaatcccg acactcaccg gagttcttca
     1441 gattttcacg taaaagaggc cacgatgccg cagccaacgt tgatgaccga ctggacatgc
     1501 atcgccacat cagggccgac cgctgacggt cgcgaaattg atccgcagtg gctgattgat
     1561 gccgctgaaa cctattcccg caacacctac acggcgatgc tgtggccctg ccatgagaac
     1621 gacaccagtt accgccagta caccttcaat ctgggcgaag tggacgcgct gaaagtggaa
     1681 acccgcgagg acggtaaaac ccgtctttac gcgcgactgg tccctaatca gtacctgatt
     1741 gaagccaaca ggctggggca aaaactcttc acatcagccg aattaatacc gaactttgcc
     1801 aaaagcggac gcgactatct catgggtgtg gcggtgacgg atatccccgc cagtctgggg
     1861 acggaaaaaa tccgcttcac cctgaacaac gaggaataca cggcacagcg ggcgaactgc
     1921 cagacgttca cgctgggcag cctgtcaccg gacaccaccc ctgccccccg taaacctgag
     1981 tcattcctga aacgcctgtt cagcgcagga cagaccgaca acaccgatac cagtaagcct
     2041 gatacaggcg aggacgcaaa aatggacgaa attaaagcac tgcttgaaaa gctgatcacc
     2101 ctggtggaag gcggcagcgc cgctgccgct ggcgagggca cagcagacac accggaagaa
     2161 gcaaaagccg aagtcgcgga aatcgccgcc gaaatcgccg atgccgccgc agaggtggca
     2221 gagctggcag aaga
//
LOCUS       DAZKHK010000310         2231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKHK010000310 DAZKHK010000000
VERSION     DAZKHK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.312"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1316)
                     /locus_tag="ACHOS5_005298"
     CDS             complement(<1..1316)
                     /locus_tag="ACHOS5_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP9779935.1"
                     /translation="MRRHNGRMAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVY
                     HWCDVGQWAALLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNK
                     HTERMAEIAATRPAVYDDGVAATSGGGEGKKRRYRKNDVSGLTAEMLDAWARKNLYAY
                     QLHCRENRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREY
                     IIDFAQQFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHE
                     VASAMATHDKYRTTYFSTPSAKTHQAYLVWNGDEWRGDDPARRAVEFPKESAMRVGCE
                     CPDGIWRYIIRLEEAVAGGLSARVDIERIRNLYNPTTYAMLYGCEFVDSKDAVFKFSE
                     LVRCEVEMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAP"
     gene            1462..>2231
                     /locus_tag="ACHOS5_005299"
     CDS             1462..>2231
                     /locus_tag="ACHOS5_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9779936.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     LLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTSPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAATGEGTADTPEEAKAEVAEIAAEIADAAAEVAELAE"
BASE COUNT          521 a          694 c          589 g          427 t
ORIGIN      
        1 ggagccacaa tcacaaacgt ggaattatcc ccggaccttg acgggtcaaa gcccgcccac
       61 acctcacgat tgccgaacgg acgtgccgcc gtgggatcgt agtcacccca cgtctccatc
      121 tccacctcgc acctgaccag ttcggagaac ttaaataccg catccttgct gtcaacaaac
      181 tcgcagccgt acagcatcgc ataggtggtc gggttgtaga ggttcctgat acgttcaata
      241 tcaacccgtg cagacaggcc acccgccacc gcctcttcca gccggataat gtagcgccag
      301 atcccgtcag ggcattcaca gcccacacgc atcgcacttt ctttcggaaa ctcaaccgca
      361 cgacgcgccg gatcatcgcc gcgccactcg tcaccgttcc acaccagata agcctggtgg
      421 gttttggcgc tgggcgtgga aaagtacgtc gtgcggtatt tgtcgtgggt cgccattgcc
      481 gaggccactt catgcagtcg cgtgaatttt gggatccaga acacctcatc gccgtaaagg
      541 tgaccgttaa atccctgggc ggtactggca ttggtggaca ggaaacgcag cattgcacca
      601 ttgctcagac ggatatgctt acccgtgagc gtgatcccga actgctgggc gaaatcaata
      661 atatattcac ggaagatttc cgcctgtacc cgtgatgcag aaaagaaaat ctggttatca
      721 ccggtcacca ccgcatcttc gaacgcttcc catgcaaaat accaggtcat gcctatctgg
      781 cgactcttga ggatgaaacg ccgctcacgg tgccggttct cgcggcagtg cagctggtag
      841 gcataaagat ttttacgcgc ccatgcatcc agcatttcag cagtcagccc ggaaacgtcg
      901 tttttccggt agcgccgctt tttcccttca cccccgccgc tggtcgccgc cacaccgtcg
      961 tcgtacactg ccggacgcgt ggcggcaatt tccgccatcc tttcagtatg tttgtttttc
     1021 tgggcgatga tcttcacgtg ctgtgcgata agttcacgca gttcggcaag ttcacactct
     1081 gtcttgccct cccgtccggt cagcacatca atacgccggg cgatcacgtc ctcaacggat
     1141 tcagcgggca gcagggcggc ccactgcccc acatcacacc agtggtaaac cgtgcgcggc
     1201 ggaataccga gttccgccgc aatatcgcgg gggtgccacc gcttcaggta aagcgatcgc
     1261 gccgcctctt tgatttcgtc agaatattta gccatgcgcc cattatggcg gcgcatccgg
     1321 cccgcattca ggcagaaaat ttcggctgtg ttcggctatc ccgcgttatc cgaagacagc
     1381 cgaattcagc cagtagcccc ccgctgtaaa aatcccgaca ctcaccggag ttcttcagat
     1441 tttcacgtaa aagaggccac gatgccgcag ccaacgttga tgaccgactg gatatgtatc
     1501 gccacatcag gaccgaccgt tgacggccgc gaaattgatc cgcagtggct gactgatgcc
     1561 gctgaaacct attcccgcaa cacctacacg gcgctgctgt ggccctggca tgaggaagaa
     1621 atcggacacc gccagtacac cagtaatctg ggtgaagtgg actcactgaa agcggaaacc
     1681 ggcgaggacg gtaaaacccg cctctacgca cggctggtcc ctaaccagta cctgattgaa
     1741 gccaacagac tggggcagaa gctgttcaca tccgccgaga tagtggagaa cttcgcccgg
     1801 agcggacgcg actatctcct tggcgtggcg gtgacggata tccccgccag tctggggacg
     1861 gaaaaaatac gcttcaccct gaacaacgag gaatacacgg cacagcgggc gaactgccag
     1921 acgttcacgc tgggcagcct gtcaccggac acctccccgg ccccccgtaa acctgaatca
     1981 ttcctgaaac gcctgttcag cgcaggacag accgacaaca ccgacaccag taagcctgat
     2041 acaggcgagg acgcaaaaat ggacgaaatt aaagcactgc ttgaaaagct gatcaccctg
     2101 gtggaaggcg gcagcgccgc tgccactggc gagggcacag cagacacgcc ggaagaagca
     2161 aaagccgaag tcgcggaaat cgccgccgaa atcgccgatg ccgccgcaga ggtggcagag
     2221 ctggcagaag a
//
LOCUS       DAZKHK010000311         2221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKHK010000311 DAZKHK010000000
VERSION     DAZKHK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.313"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>2221)
                     /locus_tag="ACHOS5_005300"
     CDS             complement(<1..>2221)
                     /locus_tag="ACHOS5_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9779937.1"
                     /translation="YPNTALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQ
                     YSGIWDGTFKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVP
                     DGFGGTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTY
                     NRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRN
                     VTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYA
                     GISTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVK
                     VSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGA
                     HFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAAD
                     DGSERLVSTARTTETTYRFRQLALGNYSLTVRAVNAWGQQGDPASVSFRIAAPAAPSR
                     IELTPGYFQITATPHLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASI
                     NIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKV
                     DLTEDNASRLDEFSKEWKDANDKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLS
                     QFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTS
                     "
BASE COUNT          440 a          715 c          571 g          495 t
ORIGIN      
        1 gatgtcaggg aaaataccgg aggactgcca ccgctggtaa tggtgggtgc cgtcaggcgt
       61 ttcaggaaca cgtcgttcat gaatatctgg ttgccctgcg ccacaaacat cggcgtttca
      121 ttcccgtttg ccgggtcaat aaacgcgata cggttagcag caaccagaaa ctggctcagt
      181 ttgccttcct ccgtgtcctc catactgagg ccaatacccg cgacataatg tttgccgtct
      241 ttggtctgct caattttgac agcccacatg gcattccatt tatcgttggc atccttccac
      301 tctttcgaaa actcatccag tctgctggcg ttatcctccg tcagatcgac tttttccagc
      361 agctctttac cgagatggga ttcggttatc ttgcctttga aaaaatccag gtaaccttcc
      421 gcatcatcgc tcgcccgacc gacggcctcc acaaatgccg atttgccaac ggtgttcaca
      481 ctgcggatat aaaagtaata atcatggccc ggtttgatat tgatactggc ggctatccag
      541 tacagccccg tgccaaggta gcgggctgtg gtttcaacct gcctgatatc ggtaatccgc
      601 gtttccgaga accagaactc aaactgtaca gtcggatcat aaaccgcaag atgcggcgtg
      661 gcggttatct gaaaatagcc cggcgtcagc tcaatccgcg atggcgctgc cggtgcggca
      721 atccggaacg ataccgacgc cggatcgccc tgctgccccc aggcatttac cgcccggact
      781 gtcagactgt aatttcccag agccagctgc ctgaagcggt atgtggtttc cgtcgtccgg
      841 gccgtgctga ccagccgctc actgccgtca tccgctgcca cggtcaggcg aagcataaag
      901 ctcacgccct tcaccacctt cggcgtatcc cagcgcgcca gcacctggta ttccccgctg
      961 tctgcggtga cttctgcggt caggtgttgc actgctggcg gagtgacacc attcaccgtg
     1021 ccgctctggt cgccgtcaaa gtgcgccccg ttatccacga tggcctcttt ctccggtaca
     1081 tgctgcacgg cagtgatggc atacgtgccg tcgtcgttct cacggatact cacgcagcgg
     1141 aacaggcgct ggcgcaacgt cggcaacttc agcccccaca cgctgtattc agcaacgccg
     1201 tcgggaacac ggctcacttt caccttcacg ccgtcggtga cggactggac ctccacgctg
     1261 accggattac cctcaccgtc aaccaggctt atcagcgtgg tgccggagga tggcagcgtg
     1321 atttcacggt cgagcgtcag cgtccgggtc tggctgttta ccgccagcac gcgcccgccg
     1381 gtgctgatac cggcatagtc atcatcacag atttcaatga catcgcccgg cacatggcga
     1441 agcccttctg cgcccacgct gaagtccacg gtctgcgttt ccagcagttc tgttttaatc
     1501 agccacagcc cggcgcggtg tgcctgcccc cggctggtac agccaaaagc atccatcttc
     1561 gtgacgttac gaccgtaacg ggcaatggcc tgcgtatcct ccacaagctc tgtcgccgtc
     1621 tcccagccgt tgttcgggtc aatccagttc acctcaacgg cattatggcg gtctttcagg
     1681 gcgctgaagc tgtagcggaa cggcgcgcca tcatccggca tcaccacatt actgcggtta
     1741 taggtccaca ccttatccga tggccggtcc tgcacaaacg tcagcgtctg cccgttccat
     1801 accggcatac agcgcatcgc cgagcagaaa tcactgagca catcccacgc cttgcgctgc
     1861 gtggtcaggt acgcattaca ggtaatacgc ggctccgtgc caccaaagcc atccggcacc
     1921 gactggtcgc aattctggcc gatgacatac agcgcccatt tatccacatc cgccgcacca
     1981 agacgtttcc ccatgccgta gcgcggatgg gtcagcatat cccacagaca ccaggccatg
     2041 ttgttgctgt atgctggctt aaacgttccg tcccagatac cgctgtattg ccgcgtctgc
     2101 gggttatagt tcgacggcac ctgcagaatg cgtccgcgaa gatgataatt acggctcacc
     2161 tgctggctgc cgaactgctc cgagtccacc tgcacgccga ccagtgccgt gttcgggtag
     2221 c
//
LOCUS       DAZKHK010000312         2214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKHK010000312 DAZKHK010000000
VERSION     DAZKHK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.314"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..255
                     /locus_tag="ACHOS5_005301"
     CDS             <1..255
                     /locus_tag="ACHOS5_005301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779938.1"
                     /translation="KIFINSENPRGRRPVACWIAGKDPKNENNYHLQQESNLIYYGAC
                     YYVPAQVRRLPSFHTEASSNENQKYSRYLPCNIILQLPGI"
     gene            complement(417..599)
                     /locus_tag="ACHOS5_005302"
     CDS             complement(417..599)
                     /locus_tag="ACHOS5_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779939.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFANHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            complement(806..1132)
                     /locus_tag="ACHOS5_005303"
     CDS             complement(806..1132)
                     /locus_tag="ACHOS5_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP9779940.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            1613..1906
                     /gene="iss"
                     /locus_tag="ACHOS5_005304"
     CDS             1613..1906
                     /gene="iss"
                     /locus_tag="ACHOS5_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFP9779941.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEARVYCSK"
     gene            complement(1938..>2214)
                     /gene="rzpD"
                     /locus_tag="ACHOS5_005305"
     CDS             complement(1938..>2214)
                     /gene="rzpD"
                     /locus_tag="ACHOS5_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage endopeptidase RzpD"
                     /protein_id="HFP9779942.1"
                     /translation="ALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSVREAT
                     TASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
BASE COUNT          604 a          561 c          464 g          585 t
ORIGIN      
        1 aaaattttca taaatagcga aaacccgcga ggtcgccgcc ccgtagcctg ttggatcgcc
       61 ggaaaggacc cgaaaaacga gaataattat cacttacagc aagaatcgaa tctgatctat
      121 tatggtgctt gctattatgt gccggcacaa gtgcgtcgtt taccgtcatt tcacacagag
      181 gcatcatcaa atgaaaatca gaaatattct cgctatctcc cttgcaacat catccttcag
      241 ttgcctggca tttaaatcct cgcccaatgt gctaccagga ccaacgaatc aactaactgc
      301 ggtagaaagt aaaattatcg gacattttta tgccccacac agtgcattac ccggaacaac
      361 catcacaggg acatgtgacg cctcccccgt cccgggatgc acctgtccgt tttgtactat
      421 gctgcgtagc caaaaccgat aacatccgca tttacctggt attccatgat gagttcacgc
      481 agcgactgat agaggaagga aagatggtta gcaaaagcaa ggcgcattgc cgccgcatgc
      541 tgcaggcatt gcaacagacg agagcaggta tttttgacca gttggaaaac tgccagcata
      601 ctttgcccga gtatatcgcc atctcatcgg aaaccagtgc aactcttatt catcgggttc
      661 cactagagaa aaagaagaaa tgaacagtga ggcgttgtgt ggcatacaac gccttcttcc
      721 atcattcctc gtcagccatg acaaaaatat aaccgctggc tctttcattt ttctcctgct
      781 tccagcccct ctctacctgg aagcatcagg gacgtgacgg cgtaaagata aattgtctct
      841 tcactccctg acagggacga ttcttttcaa atcgccattt cgccatggcc ttcaccactt
      901 catcacgaaa caaattatga ggctctgagc ggagaaaaac gatccgtgtc acagtcccat
      961 cagcaccaat atcgaactta acctcaacca gccccttgat ataatttgct gcagcatatt
     1021 ccggatatcg tggatacacc gtcactaatt gccggggctc atcagctttt tgctgcgagc
     1081 atcccactgc taggacagat aacagaaaaa gtagtaaaag gcgtcttttc atttttattc
     1141 ctacgggtct tattctgaca atatatcctg tgttccagac tgccacatca ccacatcctg
     1201 tgccattatc tgactcacat tacatacatc gcatcgggat acagtagtag cactttctgt
     1261 aatacagctt ccggtttctt ccaccatcgc accgggataa atccgcgaat cattaacgcg
     1321 gtaaaaaccc ggtgtgcatc gtttttaatt attcccgcac actcacgcag aaggaattcc
     1381 ccgtcgggct acggtcatgg ttaatgcggg aatacggcga cgatacagcg cagctaaaag
     1441 ggtaatggac ggatagaccg gtttatttca ttctacagga ttctgagtgt ccccaactcc
     1501 ctccaatcgt ctgagctcac ctgtgtagtt ttaattttca tcaatccatt taactatcgt
     1561 ttaattgttg tcacatagga ttctgccgtt tttaacaatg caggataata agatgaaaaa
     1621 aatgttgttt tctgccgctc tggcaatgct tattacagga tgtgctcaac agacgtttac
     1681 tgttggaaac aaaccgacag cagtaacacc aaaggaaacc atcacccatc atttcttcgt
     1741 ttcgggaatt gggcagaaga aaactgtcga tgcagcaaaa atttgtggtg gtgcagaaaa
     1801 tgtggttaaa acagaaaccc agcaaacatt cgtaaatgga ttgctcggtt tcatcacttt
     1861 tggcatttat actccgctgg aagcgcgggt gtattgctca aaataattgc atgagttgcc
     1921 catcgatatg agcaactcta tctgcactgc tcattaatat acttctgggt tccttccagt
     1981 tgtttttgca tagtgatcag cctctctctg agggtgaaat aatcccgttc agcggtgtct
     2041 gccagtcggg gggaggctgc attatccacg ccggaggcgg tggtggcttc acgcactgac
     2101 tgacagactg ctttgatgtg caaccgacga cgaccagcgg caacatcatc acgcagagca
     2161 tcattttcag ctttcgcatc agctaactcc ttcgtgtatt ttgcatcgag cgca
//
LOCUS       DAZKHK010000313         2188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKHK010000313 DAZKHK010000000
VERSION     DAZKHK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.315"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(695..1845)
                     /locus_tag="ACHOS5_005306"
     CDS             complement(join(695..1584,1584..1845))
                     /locus_tag="ACHOS5_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc16 family transposase"
                     /protein_id="HFP9779943.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELAILPKGRDILREAPEMKY
                     VFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAK
                     QRYGAPRLADELPEFNIKTIAASLRRQRLRAKASRKFSPVSYRAHGLPVLENLLEQDF
                     SASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSLRMTAQLACDALQMAL
                     WRRRRPESVIVHTDRGGQYCSGDYQALLKRHNLRGSMSAKGYCYDNACVESFFHSLKV
                     ECIHGERFSSREIMRATVFNYIESDYNRWRRHSACGGLSPEQFENHNLA"
     gene            complement(1923..2156)
                     /locus_tag="ACHOS5_005307"
     CDS             complement(1923..2156)
                     /locus_tag="ACHOS5_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779944.1"
                     /translation="MREISDNMLDSVKGGMNINGLPGSTNVIDLRGKDMGTYIDANGA
                     CWAPDTPSIIMYPGEDGPSYSISSSTSSANSGS"
BASE COUNT          515 a          538 c          522 g          613 t
ORIGIN      
        1 agtatttcta atataccatc tccaatggat gtatcttcca gggtattatc tgtggggcct
       61 atgtgtatgt atattgtcaa accagttagc tcttatgctt cattccctag cccttcttgg
      121 caaatgatac tgtacttgcg taggtgtcat ctgtgacatg gttgcggtgc tattttgtct
      181 ctgttggctt aaaaagagta attaagacta tgataaaaag ttaatttcca aagaacacgg
      241 tagggcgcta atcaataatc ggatgcatgc taaatcatat tttataatat gtctgtggag
      301 atgatgctaa agtcatcaat agttcttgag gaattcatta tgactatcat tggtatcgtt
      361 cttgccagac acgtcttcgc tgttcacaat gttgcgttaa atgagaaaat gttctggtaa
      421 acccccggag agcacatgca gtgctgccag ccctgattgt acttttacct ccctgagtta
      481 tgggtacctt gctaagggag tattcaccag tgaacaacat aatacaaagt agcgtaaacc
      541 ttttgttcaa ggtcagtcag ggtatctcat cggaatggtg tgcaatttaa catcgctgcc
      601 cagcatgagt tgataggcgt gaccgtgatc cgtaccgtgg gatgtgagtt tcatacaggt
      661 aatatgatcc tacccacgta atgtggacac ggccctaagc gagattatgg ttttcaaatt
      721 gttccgggct gagaccgcca caggcactgt gacgacgcca gcgattgtaa tcactctcga
      781 tataattaaa caccgttgcc cgcattattt cccggctgct aaagcgttcc ccgtggatgc
      841 attccacctt cagcgaatga aagaagcttt ccacacaggc attgtcataa cagtagcctt
      901 tcgcactcat actgccacgc aggttgtgtc gcttcagcag cgcctgataa tcccctgaac
      961 agtattgacc accacggtcc gtatgaacaa tgacgctttc cgggcgtctt ctccgccaca
     1021 acgccatttg cagtgcatca caggccagtt gtgctgtcat tcgcagtgac atcgaccagc
     1081 caatgacggc gcgtgaccac aggtcgatga ctactgcgag atacaaccag ccctcatcgg
     1141 tacgcaagta cgtgatgtca cccgcccact tctggttcgg gccgctggcg ctgaagtcct
     1201 gctccagcag attctccaat acgggcaggc catgtgcacg gtagctgacc gggctgaact
     1261 tccggctggc tttcgcccgc agcctctgac gacgcaggct ggcggcaatg gttttaatat
     1321 tgaactccgg cagttcgtca gcaaggcggg gagcaccgta tcgctgcttt gcctcaaaga
     1381 atgccttatg gacagcggca tcgcaggtga gccgaaactg ttggcgcagg ctcatctggt
     1441 gacgacgcct gagccagaca taccagccgc tgcgggcaac ccgaagtaca cgacacatcg
     1501 ctttgatgct gaactctgcc cgatgatttt cgatgaagac atacttcatt tcaggcgctt
     1561 cgcgaaggat gtcgcggcct tttggaggat ggccagttcc tcagcctgct ccgccagttg
     1621 tcgtttaagg cggacatttt cagcggccag ttcgctttcg cgctctgacg aactcatttg
     1681 ttgctgctgt ttactgcgcc aggcataaag ctgagattca tacaggctga gttcacgggc
     1741 tgcggcggcc acaccgatgc gttcagcgag tttcagggct tcgttacgaa attcaggcgt
     1801 atgttgttta cggggcttct tgctgattga tactggtttt gtcatgagtc acctctggtt
     1861 gagagtttac tcacttagtc ctgtgtccac tattggtggg taagatcacg gagggtattt
     1921 atttaactgc cgctgtttgc actggatgtg gaactactta tactataaga aggtccatct
     1981 tcgcccggat acatgatgat ggatggagta tccggagccc agcatgctcc attggcatca
     2041 atatatgttc ccatatcttt tccacgtaga tctattacat tagtagaacc gggtaatcca
     2101 ttaatattca tccctccttt cacagaatca agcatgttat ctgatatttc tctcatttta
     2161 aaacctcctg attatttatg ttcagata
//
LOCUS       DAZKHK010000314         2118 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKHK010000314 DAZKHK010000000
VERSION     DAZKHK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.316"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..532)
                     /locus_tag="ACHOS5_005308"
     CDS             complement(2..532)
                     /locus_tag="ACHOS5_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779945.1"
                     /translation="MLYRDLLPIFSERPIFKAPEAERILREQYSIAAPTPVIQEIYYG
                     LAGYDRFQELYGDLNIDQIEWKLIELPTKNFLTIGDNATYPDFLHEVVEDFTTRKGDV
                     FMDDEIKAHWCNFGTWCEPPFFIDRALLKSNTTGLHLMEGHTRVGTLLGAVKYNFVKL
                     ANTHKIYYAQAKECIK"
     gene            complement(687..1439)
                     /locus_tag="ACHOS5_005309"
     CDS             complement(687..1439)
                     /locus_tag="ACHOS5_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9779946.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMAMDNPTLNKLSEETRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCAGTGFHSVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAFAEAQLRKVTR"
     gene            complement(1453..>2118)
                     /locus_tag="ACHOS5_005310"
     CDS             complement(1453..>2118)
                     /locus_tag="ACHOS5_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9779947.1"
                     /translation="HAPGAIRLCWHCDNQLRDQFTERLESMATDNCAHWVLSVVRRDL
                     GFDDSHVVTMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPAT
                     SIIQDKAKKVLALKVEPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHH
                     LIGHGQGGMGTKAHDLFVLPLCRKHHDELHADTVAFEEMYGSQLELIFRFIDHALAIG
                     VLA"
BASE COUNT          562 a          565 c          426 g          565 t
ORIGIN      
        1 cttatttaat acattctttt gcttgcgcat aataaatttt atgagtatta gcaagcttaa
       61 cgaagttata cttaactgcc cctaacaggg ttccaactct tgtatgccct tccattaaat
      121 gaagccctgt tgtattactt ttcagcaaag ctcgatctat aaaaaatgga ggttcacacc
      181 atgtgccaaa attacaccaa tgcgctttta tttcatcatc cataaataca tcgcctttac
      241 gggttgtgaa atcttcaacg acctcatgca aaaaatcagg gtaagtagca ttatccccga
      301 tagttaaaaa atttttagtg ggaagttcaa tcaatttcca ttcgatttgg tcaatattaa
      361 gatccccata aagctcctga aatctgtcat aacctgccaa tccataataa atctcctgaa
      421 ttacaggagt gggggcggcg atagaatatt gctcacgaag tattctttca gcctcaggag
      481 ctttaaatat gggtctttct gagaaaatag gcaaaagatc tcggtataac atccatatcc
      541 ccgtgtaaga aaaaataatt taacccgctc ggcggcgggt tcttggactc atcaacggta
      601 gacatacaaa gcccatcgtt gggaaaatct tatccatatt ttttgagaaa tgcaagcatc
      661 atgtcgccat cttcggtgaa aatcacttat ctcgtcacct ttctcaattg tgcttcagcg
      721 aatgcttctt cctgccagca ctttgttacc agtttatcaa tgacgtctgc atatcctttg
      781 taccactgat aatcagtcag gtctggtacc agcttctgga catgacgtcg tgccagtgtg
      841 gtcggtaaac gactaaaccg gtttccattg caacgcccac aaatcttata aacaggcgcg
      901 ccatgaagcc gggttctttt ttcatccagg acaatgcctt tacccttaca ccctctgcaa
      961 gccgtgctga cttctccctt accatggcaa tgctgacata attccttcac ccactcttcc
     1021 ttgataacag attccccgct tctggagtgt ttcaccactt cgcgcaatac actatgaaat
     1081 ccagtaccag cacaatgctc acagcgagcc ttacttgccg cagacctgga ataatcagca
     1141 aaggcaaaac tcacaaggta agggatgatc tgtaaccggg tttcttcact caatttgttc
     1201 aatgtcgggt tatccattgc catcgcgtaa ttgagcagac cttcaatcgc aaactgagga
     1261 tcctgaacac caacttttgc caggaataag gcaaacccaa gcggtgcttt cgactgcacc
     1321 atcccctgcg cagccatcac atccgtaatc gttaaaccac ctgagcctgt cgccggtgcg
     1381 tcatcgctca attttggaga ttttggggag taatattttg gtaaggcttc aaggttcatg
     1441 ctcgttctcc acttacgcca gtacgcctat tgccagcgca tgatcgataa aacgaaatat
     1501 cagctccagc tgggagccat acatctcttc aaatgccacg gtatccgcat gcagctcgtc
     1561 gtgatgcttt ctgcacaaag gcaacacaaa gaggtcatgc gcttttgtac ccattccacc
     1621 ctgaccatgg cctatcaggt ggtggggatc atcagcaggc tttccacaac atgcacacgg
     1681 ctgtgtctta acccagcgcg tgtacttttc attaacccag cggcgacgtt ttgggcgtaa
     1741 cataaaagac tccggcgact ctggctccac tttcagcgcc agcacctttt tcgctttatc
     1801 ctggataatg ctggtggcag gaaccgaagg aacaaggtca ctctcccggg tgacagacgg
     1861 cacaaccggc ttcggtaatc tcagtgcctt acgggctgca ctttccggta aggcatccgc
     1921 caggtcatta cgaaccagcc accagcacag ttccggcatt gtcactacgt gactgtcatc
     1981 aaaaccgaga tcccgacgca cgacagataa cacccagtgg gcacagttat ccgttgccat
     2041 tgattccagc cgttccgtga actgatcacg caactggtta tcgcagtgcc aacacagacg
     2101 aattgcgccc ggcgcgtg
//
LOCUS       DAZKHK010000315         2005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKHK010000315 DAZKHK010000000
VERSION     DAZKHK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.317"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..269)
                     /locus_tag="ACHOS5_005311"
     CDS             complement(<1..269)
                     /locus_tag="ACHOS5_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9779948.1"
                     /translation="MGRPDWRAMLAGMSSTEYADWHRFYSTHYFHDVLLDMHFSGLTY
                     TVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAGGVR"
     gene            complement(296..718)
                     /locus_tag="ACHOS5_005312"
     CDS             complement(296..718)
                     /locus_tag="ACHOS5_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9779949.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(734..1474)
                     /locus_tag="ACHOS5_005313"
     CDS             complement(734..1474)
                     /locus_tag="ACHOS5_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779950.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            complement(1482..1877)
                     /locus_tag="ACHOS5_005314"
     CDS             complement(1482..1877)
                     /locus_tag="ACHOS5_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP9779951.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(1874..>2005)
                     /locus_tag="ACHOS5_005315"
     CDS             complement(1874..>2005)
                     /locus_tag="ACHOS5_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779952.1"
                     /translation="VKIPMAVPLTTAFKQNIERIRRERLPKELGYALQHQLRMVIKR"
BASE COUNT          430 a          620 c          499 g          456 t
ORIGIN      
        1 cgaacgcctc cggcaagccc tgccgctttc tgcatcagca catcatcttc aggctcttcg
       61 tcagcctcgc gccggttcag cagactgaaa tccagcggat gcatctccgg atcgctgaaa
      121 aacaggctga gcacggtgta cgtcagcccg gaaaagtgca tatccagcag aacatcatga
      181 aaataatggg tactgtaaaa gcggtgccag tcggcatact ccgtggatga catcccggca
      241 agcatggcgc gccagtcggg tcgccccatc tcacgcgcca gtttcagggc aaaactcagc
      301 tcaccgtcga acactttccc gcagaaacag gctctgcagg cccggcgtcc tctgtctgtt
      361 caggggcatt attcaccaca aactcataca taccagatag ccggtacacc acgttttcag
      421 catgagaaat tgcctctgtg ggccaggtgg taagcacttc ctgctcaatc tgtttaacgg
      481 cttcattcat ggacggcatc tgcgtcttct gcggatggtt atgccacagg gacatcgcca
      541 ccagaaacgc gccggttctg atggcgtctt ccacagtaaa cttccggttg ctgtctgact
      601 ccgcctgttc tgcctgccgt ttcatcaggg cgagatgctc aatacgctgc agggctgaca
      661 gttcagaaag cgtgacggtc acaccgttat gttcaaatga ttcggttttc aggaacatct
      721 ctgactctcc ggattaactg gcggtgacgg taatttctgc aaccgcagca aattcaccat
      781 taccggatac aaccggaatg ttgaccttgc ctgcagcaac gccgttcacg gtgatggtca
      841 taccactgac cgacacggtg gcttttgttt tatccgcaga caccgcacga aagctcttgt
      901 cggttacgcc ctccggctgg aaggccacgg tcagcgtggt gctctgccct ttcaccaccg
      961 aggtgctggc aggcgtcacg gtcatgccgg ttgccgctgt taccgtgctg cgatcttctg
     1021 ccatcgacgg acgtcccaca ttggtgactt tcaccgtgcg ggtgatcact tccttcgccg
     1081 tcaccgcctt accgatactg ctgacccagc cacggaacac atcgaccgtg ccgttcggga
     1141 agcggatttt ataggcacgg gtatcgcctt cattaaacca cgccagcagc gcctgctgcc
     1201 cctgctctcc gggcatccac gccagcgtga agctggtatc tccggcagat ttctgcccct
     1261 gcccggtcgc agtccagtct gcatcttcat catcgagata gctgtcgtca taggactcag
     1321 cggtcagttc gccgggcgtc aggtctttaa cttttgccag acgcgaccag tcaacgtctg
     1381 aaagcgggtt cgcataaggg tcaccgctcc ccttataaac ccacagggtg gtcccggcac
     1441 ctttcaccgg cattacagga tttggtacag gcatagcgtc ctcacatttc ataggtaatg
     1501 acataagtca gatcggctga actccacagg cccgcatcat cgtcgcgccg gtagtcatag
     1561 ccgctggcca ccatactggt gatcaaatct gacagtgccg ggatatcact catcaccgga
     1621 taaatccggg actccatcca cgcatccagc tctgaatccg gcacctgagc aggcaggaaa
     1681 acttcgatat gcagctccgc ctgccaggta tcgctgtcca gctcttcgcc cgtatattca
     1741 gcgccggtga gataaacggc aactgccgga aaatccgcct catcaaaaac agcggggcga
     1801 ccatcaaaaa acgtcgcccc ggtgtcatgc ttctccagtg catccagtac ggctgcacgg
     1861 agttcagtat gtttcatcgc tttattacca tcctcagttg atgctgcagc gcatagccca
     1921 gctctttcgg aagacgttca cgccgtatcc gctcaatatt ttgtttaaac gccgtggtca
     1981 gcggcaccgc catcgggatt ttcac
//
LOCUS       DAZKHK010000316         1952 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKHK010000316 DAZKHK010000000
VERSION     DAZKHK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.318"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..1564)
                     /gene="mobQ"
                     /locus_tag="ACHOS5_005316"
     CDS             complement(44..1564)
                     /gene="mobQ"
                     /locus_tag="ACHOS5_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFP9779953.1"
                     /translation="MAIFHLDFKIVKRSEGMTSVAKAAYHARTRITDDRIGETFDFSH
                     RTDLHGHIILAPVSAPAHIIESSSALWNEVERVERQNNGQTARYFDVAIPVELNNDDK
                     KKLVAEYCQKNFVDKGMIADIAFHDLDGKNPHAHVMLTLKTIGPEGFGKKDRSWNDKK
                     VMLQWRESWATMSNSYLEAAGCEERIDHRSLRTQCADALAQAEEAFSAEEKAFWLAKA
                     TETNRPAMQRVHRAKWNDAESQEQRSAEQAQRDHQIEEAKKIYTTFSELPLEIVVDVR
                     SFTITHLAEPEEIVLPDSPATVEQQPAMETPTTSRRPAAKSYRDPNKVSKVSAAGKKS
                     PVLVVPEPNKSTKLKTPSLQNTRAMKRAPVRSRKQTKPRQSGLFKRFTLLIVGYIREK
                     FIWARRKPDTIDADHDKRIAENYIYDEVLGVSVPRSEFERRAKFNNDQTSPEAGIYGR
                     VSDNDETVRFPSRPTHEQPNFDRNMDLTPSSPSEYSGKSHVSKLRPLAYRNNNYNE"
BASE COUNT          509 a          408 c          434 g          601 t
ORIGIN      
        1 aggccagtta tggagcttaa ataaaaatgt attttctatt tcgctattca ttataattat
       61 tattacgata tgccagtggt ctcaactttg aaacatggga ctttcctgaa tactctgagg
      121 ggcttgatgg tgtgagatcc atgtttctat caaaatttgg ttgttcatgt gtaggacggc
      181 ttgggaaacg aactgtctcg tcattatctg atactctgcc atagattcct gcttctggtg
      241 atgtctggtc attgttaaac ttagcgcgtc tttcaaactc tgagcgtgga acagagacac
      301 ccagcacttc atcatagata tagttctctg cgatacgctt atcatggtca gcatcaatgg
      361 tatcgggttt ccttctggcc catataaatt tctctcttat atatcctact atcagtagcg
      421 tgaaacgttt aaacaaaccg ctctgacggg gcttggtctg tttcctactc ctcacaggag
      481 cacgtttcat cgctctggtg ttttgaagag atggggtttt caatttagtg gatttatttg
      541 gttcaggaac aaccagaaca ggggatttct tccctgcagc actaacctta ctgactttat
      601 tgggatcacg gtaagatttt gctgctggtc ttctgcttgt ggtaggtgtc tccatagcag
      661 gttgctgttc tactgttgca ggggagtctg gaagaacaat ctcttcgggt tcagcaagat
      721 gtgttatcgt aaagcttctg acatcaacaa cgatctccag tggtagctca ctgaatgtgg
      781 tgtagatctt ctttgcttcc tcaatttgat gatcgcgttg agcttgttca gcagagcgct
      841 gttcctgtga ctcggcgtca ttccatttag cacgatgaac acgttgcatt gccggacggt
      901 ttgtttccgt tgctttggca agccagaatg ctttctcttc agcgctgaat gcttcttctg
      961 cctgagcaag ggcatcagca cattgagttc tgagagaacg gtgatcgata cgttcttcac
     1021 agcctgcggc ttcaaggtag ctgtttgaca ttgtggccca cgactcacgc cattggagca
     1081 ttaccttctt atcattccag cttctgtctt ttttaccgaa gccttcggga ccaatggttt
     1141 tcagggttaa cataacgtga gcatgaggat ttttaccgtc taaatcatga aatgcgatat
     1201 cagcaatcat ccccttatcg acaaagtttt tctggcagta ttcggcaacc aattttttct
     1261 tatcatcgtt gttgagttct acaggtatag caacatcaaa ataacgggct gtttgaccat
     1321 tgttctggcg ttccactctt tcaacttcat tccagagagc tgaggagctt tcaataatat
     1381 gagcaggtgc ggaaacgggc gctaatataa tatgaccatg aagatctgtt ctgtggctga
     1441 aatcaaatgt ttctccaatg cgatcatctg taatacgtgt acgggcatga taggcagctt
     1501 tcgcaacgga agtcatgccc tcgcttcgtt tcacaatttt aaaatccaga tgaaaaatcg
     1561 ccattaaaaa tataacctca aataaaaatg ctgctgttag tttttcgcca gaaaaacttc
     1621 ggggttgacg ttgaccttgg ttttgctttt agcctgcggc aggcaaccgt cggtagaccc
     1681 cacacactgc gtagcagtgt ataggtgggc attgtcttta agaaaataag ccaatgtaaa
     1741 atgcccctct tattaattaa tacgaaatat atgtatggtt gtaaaacaat acgccggaat
     1801 aagcggcgca aactggatta aaggtgtagg tttcacatcc acaggacaaa aagagtaaag
     1861 aaaaggcccg taagggcaat agcagtatta attattcccg ccgtaaggcg ggatagctaa
     1921 atttgatgaa aaaaaccaat aaaacagaga gt
//
LOCUS       DAZKHK010000317         1951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKHK010000317 DAZKHK010000000
VERSION     DAZKHK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.319"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(766..1503)
                     /locus_tag="ACHOS5_005317"
     CDS             complement(766..1503)
                     /locus_tag="ACHOS5_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9779954.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVIQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRVWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIEGRIAIK"
     gene            complement(1726..1867)
                     /locus_tag="ACHOS5_005318"
                     /pseudo
     CDS             complement(1726..1867)
                     /locus_tag="ACHOS5_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          608 a          396 c          393 g          554 t
ORIGIN      
        1 atatgtttta tgtatttcgc atggtgatga tttatacacc tgcactgaat ttttctgtat
       61 ttccagaact gaatcgataa acagaattgg taataatgaa agttaatcac ataaaaaaga
      121 ggttaccgcc ttataatgat aagagttcat tatccattcc atcccgacaa aacctgcctg
      181 tgtataaaaa acaagaacca tgtcacaaaa acattacatt ccggcaagtt atgacagaat
      241 tactgaaaac agtcatctgt agtaattgtc gtttctgata tatgctcatc catccggaaa
      301 tacgacatgg tcctgactgg cgttcacttc aaagtgtaat ttattattca cacactgaga
      361 ctgtaaaacg gatggcgtgg atgagtacac agtctgaaga gtataaaagg aacagctccg
      421 gattgttacc cgggattact atagttcacc acacaaccgt aaacctggtt gtgtggtgaa
      481 acagtaacat attaagaatt tgtgttacca cctgttttct caggaaacgg attgcaggag
      541 aggtatactc attcatcttc ccggagtttt gttaatcact gcataatcaa acggaccaat
      601 acattctttg ctcgcatccg taaaaatgtc gccacaatat catcagtcag tcaactcatt
      661 accatctgtt ttccgggtcc gtagttacta cagtgctccg gagtgataaa cagaaactga
      721 taatgaagca taagaatact tttattatgc gctgactgac atactttact taattgcgat
      781 tctgccctca ataaatccgg actgattaat catcatctga tgaggtgtta tgccaaaata
      841 ttctctgaaa acgctgataa aatacggtgt gctggcataa ccgcaacgta ttgcgatttg
      901 attcactgac agtttactgg aaataagttg tcgggcgtta tgcatgcgta tatccagcag
      961 gatttgggag aaagaaactt tttcctcctg aagttttctt ttcagtaaac tctcactcat
     1021 gtacagagaa ttactgatat ctttaactct ccatacccgt gatggactgg aacagataat
     1081 atacctgact ttaagcgcaa cagagtttat gcatctgaat agcagaaaca tgaaacctct
     1141 gtgtgtggaa aaaatggaaa ggcaggcaaa aaacatctgc tcagaaaatt gttgtggcaa
     1201 tgtcttttgc agactgagat tctgtatgac attatgtaac agagcgatgt gagagaaggt
     1261 tgttttaatg aacggaacag aaacagcact atcagagtgc tggatcctgt cggcactttc
     1321 catatatttt ctgacaatgc attcgtcaat ccggagctca gtaacattac cgggatggcg
     1381 caggaaaaag gactggctga tgttatctgt caacaggacg tcatttttct gaaaaaatat
     1441 ctttttcccg gcaatctctg tttcaaacgg acggttaaca agaataacgt aacagacaat
     1501 catcacacat actccctgat tagcataaaa acaacgatat caaaagacta gacgcttttt
     1561 caggaaaaat cttatttgtc cggagattat tcttacgggg ccgggcgaat gctatctgaa
     1621 tcgtacggtt catagaaata aatatttacc gacatagccg gagggttttc agatgcaatc
     1681 cctgactcac tgcgaaatcg catcgtatcc gatcgcggac tcgccgattc tcagaacagg
     1741 cagtccctga gaatcgcaga acgctagtgc acgatcgttg aacacatcag ctacggtgat
     1801 cgatgtttta ctcgtattat agtttgcagt gtgccacttt cggggatgta gctacgaacg
     1861 tctgctggct tcccgatatg tcagtaaaaa atacccgccg tattacagca acggcaggta
     1921 atggcacatc cagaagagaa gtattttatt a
//
LOCUS       DAZKHK010000318         1943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKHK010000318 DAZKHK010000000
VERSION     DAZKHK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.320"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..849)
                     /locus_tag="ACHOS5_005319"
     CDS             complement(<1..849)
                     /locus_tag="ACHOS5_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9779955.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVS"
     gene            1006..1926
                     /locus_tag="ACHOS5_005320"
     CDS             1006..1926
                     /locus_tag="ACHOS5_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9779956.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          562 a          434 c          424 g          523 t
ORIGIN      
        1 tgaaacatat atataaaatc caggaaatga aacggcccaa tcctcgagaa tcggctgtaa
       61 cttgcccgca ttgatatgtt cagcgattgc aaaatccaga tgttgaatga tacccgttcc
      121 attcagcgcc attcgtatca tgctttcgtc atcattagaa atatacttac ctgacacctc
      181 tatttcgaac tcttcattac cccccacaac attcgaaaac tcccatgctg agagtgcacc
      241 gctactttta tgccgcaacc gaagacaatt atggttactc aattcctgag gagtttcagg
      301 aataccgtgt tcttttaaat aatctggcga tgccacagta accagtttaa taagcggaga
      361 aacaggaacg gcagtcatat gctcatccag tccttgctca agacgtatgc ccacatcaca
      421 gccgtccgca ataatgttag ataaaccgtc atccataatg agttctatct ttgttttagg
      481 gtaacgagta agaaactcgc caatatgagg ctcaacaagc atccttgctg caacacggga
      541 ggaatttatt ctcaacagtc ctgtaggttc aagttgggta tcaccgacat ctttcaacgc
      601 atcatcaatt tgccccaaag ccgacaccag cacttcatac agatgctgtc cttcctcagt
      661 aagcgacata ttgcgggtcg tacggtatat cagcttggtg ttaagacgtt cctcaagaga
      721 ttttaaattc tgggacaatg cggcccgcgt aatacccatt tcagaggctg ctctggaaaa
      781 actgccggca cgaacaatca gcgcaaacca ggcaagagaa ggcaaaattg aaggctctat
      841 tttcatcatt gtaaacctta acttaacact gtgatagata ttagaccatt gatgataacc
      901 aatacaagtc atactattac gccgttcgcg atatgaacac gaatgccagt tgcggataat
      961 agaaatgata tttaaatctg tttaggcaaa tccctggagc ttgaaatgaa aactgtttcg
     1021 ataaaacaca cttactggga tatagctgct gatatctatt tccctgacga ttttaatagt
     1081 gaaaaaaagt atcctgccat tatcagtgcc catccaattg gaagctgcaa agaacagaca
     1141 tcgggtagtg tgtatggtgc cgccctggca aaagctggct ttatcgtcat tgcttttgat
     1201 gccagttttc agggaagcag cggtggagaa ccgcgttatc tggaagatcc aacaatgcga
     1261 gtaaaagact tcagcattgt cgttgattac cttaccacct taccttacgt tgatgccggg
     1321 cgtattggtg tactgggtat ttgtggtgga ggtggatatg ccattaatgc cgcaatgaca
     1381 gaacgtcgga ttaaagccat tggtactgtc acgggtgcaa actacgggcg tcttatgcgt
     1441 gagggattta cagcgtttaa tcctatcgga gcactcgaag caatggcgca acaaagaaca
     1501 gacgaagcta acggcgcaaa acttcgcgta gatgatttgc tcccttcttc tccggaagca
     1561 gctcttgaag cgggattaac agaaatagac ctttttgaag caacagaata ttatcgttcg
     1621 ccacgaggct gtgcgcctaa tggggtaaat cgttcgctat tttcacatca gactgttgcc
     1681 gtaggctggg atgcttttca tcttgcagaa gtcctgttaa ctcaaccact aatggttgta
     1741 gtgggcgata gagtgggcgc ttttggtgcc tatcgtgacg gctgcgaaat tattggtcgt
     1801 gcagcctcaa aacataaaga actggtcgtg gttgaaggtt attcacatta cgacctgtat
     1861 gataagccag aacctgtgaa acaggcgctc gataaattaa ttccttttta caaaacacat
     1921 ttgtaataac cttgcaggga gat
//
LOCUS       DAZKHK010000319         1912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKHK010000319 DAZKHK010000000
VERSION     DAZKHK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.321"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(367..1353)
                     /locus_tag="ACHOS5_005321"
     CDS             complement(367..1353)
                     /locus_tag="ACHOS5_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9779957.1"
                     /translation="MYRHDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1453..>1912
                     /locus_tag="ACHOS5_005322"
     CDS             1453..>1912
                     /locus_tag="ACHOS5_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9779958.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNS"
BASE COUNT          563 a          451 c          363 g          535 t
ORIGIN      
        1 acctgcgcca catcgactga atgttttggc cccgacagaa aaaagaacta ataggtgatg
       61 tttccgtcca gagatactct ggatggagtg tcggcgtcag tgatataaga ctcgagtgaa
      121 cactgagccg acctcgaaat gacgttgtag gtgcagacat gccataatgg gactgtcgat
      181 gatattacag ggagtcacgt cccctggtgc gtgccttcgt gacacactac cgtccacata
      241 gtatcagcac cagtgatatc aaacggtaac gaccagctat cctggtgttg ataccgacaa
      301 ctccatacca ctccgtcatg tctctcagag aatcatgtca gagctgtaga tatatcctga
      361 acttgtctac aaataatttc ttattttttg cgcataacat tcagcaataa ctccccttct
      421 ggccattgaa ttaaagtgtg tacttctctg tgacgttgtc cagcttaatt ctgtcctcca
      481 tgatgacccc agatacccga cagataaatc atcggcatct tcttctggct cattagttaa
      541 ttcatgtcgg ttactatcct gaaagctgag aaaatgtatt tcatcatcag tccgttgtct
      601 gtaatgtcca tatataaaat catattcttt ttgataagat tccttccagt accaggttgt
      661 atcaccacac aaccatggaa ctctttctga cgaattacca acacactgag atgagtattc
      721 agataactca gacctgaaag aagttaccat ctgataaaac aagtcaggat gcttttcata
      781 atctggtgac ataagatcaa actccccctg catccagcag acagatagca gtttattctt
      841 tctgttatat tccagagcta ccttaacccg ggctactaag tcctcataga gtgctgttcc
      901 ggtgccccac cgcattgcat cagctgttgc ccctgtatca ggtacataca taccttcagc
      961 tccaacagta aaggcggcac ctcctctgca acaaggaacc agtaatatcc ctgcttgctc
     1021 aggtatgtat ggcagtaact ttttagctat atgcaatccc tgtccgacac aaccatattc
     1081 ccctttatgc aaatcagcag ccggatggtg atatccactc atatcctgaa catcatgcag
     1141 gcagtgatct gccggaataa tctcattgaa tttacactct tttcctcctg gtgtgattgt
     1201 ttttcgacga gccagttgtt ttacacggga ctcgggtttg tcgagtgtat ctggcagggg
     1261 aataccttca ccgtaggcca taccattaga ctgtcctgcg agagctatca cataccagaa
     1321 atctattttt tcattaaaat catgacgata catcactccc cctttacata atagatttaa
     1381 ttaaatattt tcattattaa aagaaagtgt taaattaaaa actcatttgc tctaatccat
     1441 ataaatcaat atatgtccga caacaaaaaa accgtgttac ccgggctaac aacgaccaga
     1501 aagcccattg ttacaaaaaa actttcagaa atcgttgccg aagagcttga aagaatgatc
     1561 cgccaggagg agataaggga aggtgaatac ctgccttctg aacgtgaact tatggcattc
     1621 tttggcgttg gaagaccatc tgtcagagag gctttatcat ctctcagaca taaagggcta
     1681 atccggtata acaagggaga aaaagccagt gtctgccgtc catcaccaga aaccatcatt
     1741 aatgggttat caggcattgt taaagatttt ttatccacag ctgatggcat caaggatttt
     1801 gaaagactac gcatcttttt tgaaaccaac cttgtaagat atgcagcaaa aaatgcaacg
     1861 cctgaggata ttcacaggct tgagaaggtg ctcagtatcc atcataactc ac
//
LOCUS       DAZKHK010000320         1912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKHK010000320 DAZKHK010000000
VERSION     DAZKHK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.322"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            272..733
                     /locus_tag="ACHOS5_005323"
                     /pseudo
     CDS             272..733
                     /locus_tag="ACHOS5_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            1099..1482
                     /locus_tag="ACHOS5_005324"
     CDS             1099..1482
                     /locus_tag="ACHOS5_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779959.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     RQVYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVVVREQDMKKMEAEFGRIIKIH"
     gene            1762..1911
                     /locus_tag="ACHOS5_005325"
     CDS             1762..1911
                     /locus_tag="ACHOS5_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9779960.1"
                     /translation="MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
BASE COUNT          557 a          348 c          451 g          556 t
ORIGIN      
        1 tgcggtcttt cattatgaag ttatcccata tatctgaaag gtaattaagg ctactattaa
       61 ctaaaaaaca tacgttaacc ttcgtacaga atgcatgatt aattcatgag accttagtaa
      121 cacatggcta tatgtgtatt cagccactga atcttatttt actgaataga ccacaattta
      181 acggtttatt tttggcgtga tgagtgaggc attattcatc acggaatgga attcctgcaa
      241 tcacaaaaag taatcagtga cggagagtaa aatgaagaaa tatataccag tggtattgtt
      301 tgttttttca tatcaggttt tttctgctga tattcatgga cgggtcgtgc gtgttctgga
      361 tggcgatacc attgacgtca tgttatctca gcatccggtg cgtgtccgtc ttgttaatat
      421 tgatgcacct gaaaagaaac aggactatgg acggtggact gaaaaaataa tgtaatctct
      481 ggttgcggga aaaacggtta ctgtcacata ttttcagcga gatcgttatg gtcgtattct
      541 cgggcaggtt tatgcaccag acggaatgaa tgttaatcag tttatggtcc gtgcaggcgc
      601 tgcatgggtt tatgagcagt acaacaccga tcctgttctt cccgtcctgc aggaagaagc
      661 acgacagcag aaacgtggtc tgtgggcaga cagcgcgcct gttccgccct gggtatggcg
      721 ctcacggaaa taatgatatt tctgtggcta ttgagttctc attgtaatgc gatatttaaa
      781 agcatgaagc atatttcgtt attaactggg gtcagtttgg atatagagaa ttattgtacg
      841 gtaagcctgt tttttgaaca ggcttaccta tagcaatagg gtgtactccc gcgctatacg
      901 gtgttttcag gagaattggt cttaatcgga taacctaaat gcgatacagc aagagcgaca
      961 acttaaaagc gataactgct gacgtaaaat caatccgtta cacatcggga ttggttaaat
     1021 aatgcgctta acgtacaaaa atttccgatc tccaaactga ccccttaact tatctctctg
     1081 ttaatgaaag gtgaacgaat gaactatcag gcagtatcag aattaataac atcgtctaat
     1141 cataatgttc ttatagtggg ggactcagct tctgaagttg atagtttttt aaataaactg
     1201 aatatcactg attataagta ttatgatttc aggcaggttt acagttgttc tgacagaaca
     1261 ttaaatgatt atgcggttat ttttatcagg gatgcactga acgccagtga gcacattatt
     1321 atttttaatt gtacaggatg gcctgatttg aataatgaat ctgccgtcat gcagtttgcc
     1381 agagtggcac gaaaatcagg aaaacaactg atagtcgtag tgagagaaca ggatatgaaa
     1441 aaaatggaag cagagtttgg aaggatcata aaaatccact gacgccttcg cgttgctcag
     1501 ttgtccaacc ccggagacgg gaaaaagcaa gttttccccg tccccggcgt ttctgtagtg
     1561 aaaagcggga aatggaggga atggtcagta acagggaatg tggtattatc gcggcgggtg
     1621 tctgagcctt tctggttcag gcaagacgca ggtaccagaa atgcgaagac cccacttgtt
     1681 aatccattaa ctcgtgaggc ctgcatgaag tatctcaaca ctactgattg tagcctcttc
     1741 cttgtagaga ggtcaaagtt tatgacgaaa tataccctaa ttgggttgct cgccgtgtgc
     1801 gccacggtgt tgtgtttttc actgatattc agggaacggt tatgtgagct gaatattcac
     1861 agaggaaata cagtggtgca ggtaactctg gcctacgaag cacggaagta ag
//
LOCUS       DAZKHK010000321         1891 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKHK010000321 DAZKHK010000000
VERSION     DAZKHK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.323"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..147
                     /locus_tag="ACHOS5_005326"
     CDS             <1..147
                     /locus_tag="ACHOS5_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9779961.1"
                     /translation="TYRKDEVIPLQRKIMNAVNSDPDIPEHLYLRFDVEAGHVAEEKA
                     VPVG"
     gene            185..448
                     /locus_tag="ACHOS5_005327"
     CDS             185..448
                     /locus_tag="ACHOS5_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9779962.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(606..833)
                     /locus_tag="ACHOS5_005328"
     CDS             complement(606..833)
                     /locus_tag="ACHOS5_005328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779963.1"
                     /translation="MSHLIKRYTNYDTTLLADILSCIAANIEDAYILSGAVAGRDYNY
                     NDLMSLAVKYLSGTKQIPDIHTETIIRGEPD"
     gene            complement(1014..1208)
                     /locus_tag="ACHOS5_005329"
     CDS             complement(1014..1208)
                     /locus_tag="ACHOS5_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779964.1"
                     /translation="MNITRNSKLKEHTMNDIRRISDRELTKMIESAQQLLSRQNPVVE
                     ETWWENLLAALTELRERRQQ"
     gene            complement(1217..1621)
                     /locus_tag="ACHOS5_005330"
     CDS             complement(1217..1621)
                     /locus_tag="ACHOS5_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779965.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAANRQSRR"
     gene            complement(1618..>1891)
                     /locus_tag="ACHOS5_005331"
     CDS             complement(1618..>1891)
                     /locus_tag="ACHOS5_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9779966.1"
                     /translation="PPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALG
                     VQLHESAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
BASE COUNT          373 a          451 c          558 g          509 t
ORIGIN      
        1 acgtaccgca aggatgaggt gataccgctt cagcgtaaaa ttatgaacgc cgtgaacagc
       61 gatcccgata taccggaaca cctttatctt cgttttgatg tggaagccgg gcatgtggcg
      121 gaagaaaaag cggtgccggt cgggtaaaat gtcctccccg ggatacgtgt tatcgggagg
      181 taagatgcgg atttttaaag cccggtgtcc ggactgtggc gcacctgctg tcatccggaa
      241 gacagactgg aaaacccctg aacttgcgga tctgtactgt gcatgcagtg atgtcgaatg
      301 cgggcatacc ttcgtctgta cgatgacctt ttcccatacc ctcagccccg gtgcccgctc
      361 cggtcgcaat atggtgaagt ttcttgttga tgccatgcgc ccggaagatc gccggtatgc
      421 gcttgaacta ctgtcgcggc gttcctgacg cgcttcgctt gtctgctgct tcgccggggc
      481 aaaaagaatt tttaccccgg cgaagcagcc ctgataaagg ggggggtggt gtaaattcgg
      541 acgttataaa attaaaaaag cgccgtaacg gcgctcgggt agagtattta ttcatgcaaa
      601 aatagttaat ccggctcccc tcgtattatt gtttctgtat gaatatcggg aatctgtttt
      661 gtaccggata gatattttac agcaagactc attaagtcat tgtaattata atcccttcca
      721 gctacagctc cactcaggat gtaggcgtct tcaatattgg ctgcgatgca ggacaagata
      781 tcagctaaaa gggttgtatc gtagttcgta tagcgtttga ttagatgaga cataaaacct
      841 ccgggttgct tattaatatt gctggcgaaa tcagtcattg tgttagctat gtaatattaa
      901 cagaagtgta aagtgttttt attccggggt ggtaatgttg catatatttt cttagtgtgt
      961 gtcgggtgaa tgaattgcgt tgcggtgaaa aaatcgggcg cgtcagtgcg gccttactgc
     1021 tgacggcgtt cccggagttc ggtcagtgct gcaagtaaat tctcccacca cgtttcttcc
     1081 acgaccgggt tttgtcgtga aagcagctgc tgtgccgact caatcatttt cgtaagctcc
     1141 ctgtcgctga tcctcctgat gtcgttcatg gtgtgctcct tcagtttgct gtttctggtt
     1201 atgttcatgg tctttgttac ctgcgtgatt gcctgttggc ggccgaatgc acatagcggg
     1261 caattgccgc ttttcggtta atcgatgcca gtgttctggt gagttctgcg ttgtgtttgt
     1321 gtgcctgctc ttcgtggtac tgccgcatga tttttccggc aggcgtgagc ggctccggct
     1381 ccccgggcgg acggcgcgga cgaacagcga caagccagct gcggttagtg ctgtccggtc
     1441 gtccgttgag tatgtcggtg atgatggccg tggtggtgcg atcgggactg acgaccgtga
     1501 tttccagctc atcgcccggt tgtggccccg tggcaatgtt gttcagggtc agtaagggcg
     1561 tgcgctggcg catcatttcg tcaaagcgct ggcgctgcat ggcgcaataa aagcgggtca
     1621 ttgtggcgcc tccgtattcg gtgcgggggt gatatcaatg cggcgggtgc tgtcattcca
     1681 gtacgcggtg cgtccgtcag ccaggcgcac gcgggcaccg tgacggacgg aaacgacggc
     1741 gctttcatgc agctgcacgc ccagcgcatc aagctcgcgg gtgatttgtg cggtgatgtg
     1801 tggctcagcg gtcagagcga cactcacgcg aacgccgcca gcgggcgcac tgatgccgcc
     1861 gtttttcacg gtttccgtgt caggtggcgg g
//
LOCUS       DAZKHK010000322         1885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKHK010000322 DAZKHK010000000
VERSION     DAZKHK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.324"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..99
                     /locus_tag="ACHOS5_005332"
     CDS             <1..99
                     /locus_tag="ACHOS5_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFP9779967.1"
                     /translation="AERDYFILRERLMTMQKQLEGAKEYIRTQCIN"
     gene            123..347
                     /locus_tag="ACHOS5_005333"
     CDS             123..347
                     /locus_tag="ACHOS5_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779968.1"
                     /translation="MKKLLVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            692..1018
                     /locus_tag="ACHOS5_005334"
     CDS             692..1018
                     /locus_tag="ACHOS5_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP9779969.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(1150..1350)
                     /locus_tag="ACHOS5_005335"
     CDS             complement(1150..1350)
                     /locus_tag="ACHOS5_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9779970.1"
                     /translation="MSSKNRTSRTTTRNIRFPNHMIEQINIALQHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            1392..1757
                     /locus_tag="ACHOS5_005336"
     CDS             1392..1757
                     /locus_tag="ACHOS5_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9779971.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          456 a          406 c          507 g          516 t
ORIGIN      
        1 gctgaacggg attatttcat cctcagagaa cggttgatga caatgcagaa gcagctggaa
       61 ggggcgaagg aatatatccg cactcagtgt attaactagt atttttgtta tccggagaat
      121 gcatgaagaa attactggta accgtagaac cttttcaggg aacaattccg ttccgtattt
      181 tgcagcgtgg tcgtgttctt gttgaaggtt cgttcagtgg taaatgtacg caattacact
      241 cccggacctt tcaggtgaat gccacgaatg aagagctaac cgttgagtgt acgatgaatg
      301 ccgctaaatg ccgcatggta tccgctgcat tacagccagt gtgttgagcg accttattat
      361 ccatgcgcgg tattgtcgcc gtattcccgt attaacagag accgcagccc gacagggaga
      421 ctcctctgcg cgagtgtgcg gggataatca aaaacgatac acaccggggt ttaccgcgtt
      481 aacggagcgc ggcgttgtcc cctcatggtc gctggtccgg tgcgatggtg gaagaaaccg
      541 gacgatgtgt tacctcgcaa gctctgttat gtcatgtgtc tgatttgtga tttaagtcgg
      601 ataattgtcg ttgccattaa gcagaggatt gatgaccgac agggtggcat tgttagaata
      661 agacttattc ttatctgtgc cgggaatgaa aatgaaaaga aatcttccgt taattatttt
      721 gttgtcttct ctggttatgg gctgtacgca acataaaaca gatatgcccc gacagttggt
      781 taaggcatta ccacaatatc cggcctatgc agcggcaaat tatataaagg gacgggttga
      841 tgtgaagttt gatattggtg ctgatggtac tgtcacccga attgagttta tccgttcaga
      901 gccgcaccat ctgtttgatg agcaggttgt aaaggcgatg gcaaaatggc gatttgagaa
      961 ggacaggccg cgtaaaggcg tgaagaaaac gtttatcttt agtccttctg caccctgatt
     1021 atttcatcag aaattaatta tcactctgtt gttattctgt acatcccggc tgggtaagtc
     1081 ttgttccgcc gggtatgaag atgaaatatt gttggaggac agtgggtacc tgctcctgta
     1141 accgaacgtt catttctcgt tatttgtcat gctggccgga cgcagatgcg ttgcatctgt
     1201 tgccagcctt ctcctgcagg cttcaataac ccacgctgaa aagttaccgg accctttatg
     1261 ctgaagggcg atgttgatct gttcaatcat gtgattgggg aaacggatat tgcgggttgt
     1321 ggttctgctg gtccggtttt tcgatgacat atttatttcc tttactgatt gccatatgac
     1381 ggggatttta catggctgag cttcgtacac tccagagcag aatcaaaaca ctgaataccc
     1441 gacgggtgaa tattctgaag ggtgaacagc gtcgtgtcag tggcagtgca cgtgtttccc
     1501 tcaagcgtca tatctggctc agggatgccg ggcagtgctg tctctgtggt cgtgtggttg
     1561 acctctgtga cagtgaactc gatcaccgaa ttgcacttca gttcggtggt ggtaatgagg
     1621 agacgaatct ctggacgctc tgtaccgaat gccatcgaca aaagtctgct cgtgaagcgg
     1681 cgggtggtat gccggacccg acgctgccgg aggtgtccgg aggtagtggc agagcggacg
     1741 acattatcgg actgtaaccc gaccgggggg ggatcatccg gcgtaaaaaa cgatcgctcc
     1801 ggacaccgcg ccccctctca cgcagagaaa aaattcccct tcagaagtta taaacatgtt
     1861 tataacctac tgatggattt ttacc
//
LOCUS       DAZKHK010000323         1857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKHK010000323 DAZKHK010000000
VERSION     DAZKHK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.325"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..577
                     /locus_tag="ACHOS5_005337"
     CDS             44..577
                     /locus_tag="ACHOS5_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9779972.1"
                     /translation="MSRKFRYGLSAVVLAMIAAGASAPEILDQFLDEKEGNRTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCLPSTFYKRLNAGDRKGACESIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            574..1041
                     /locus_tag="ACHOS5_005338"
     CDS             574..1041
                     /locus_tag="ACHOS5_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9779973.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPRLADTVERDYFTLRERLMMMQKQLEGAQEYIRTQCLK"
     gene            1344..>1857
                     /locus_tag="ACHOS5_005339"
     CDS             1344..>1857
                     /locus_tag="ACHOS5_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9779974.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVE"
BASE COUNT          507 a          444 c          506 g          400 t
ORIGIN      
        1 attaaagaag accggcgtaa ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg
       61 ctatggttta tcagcggtcg ttctggcgat gattgctgca ggtgcttctg cgcctgaaat
      121 cctcgaccag tttcttgacg aaaaagaagg taatcgcacc acagcatacc gtgatggtgc
      181 gggtatctgg accatctgcc gtggtgccac ccgggtggat ggtaagcccg tcgttcccgg
      241 catgaagctg tcgaaggaaa aatgcgcaca ggttaatgcc attgagcgtg ataaggcgct
      301 ggcatgggtg gagaaaaaca tcaaagtgcc actgaccgaa ccccagaaag cgggtattgc
      361 gtcattctgc ccgtataaca ttggccccgg taagtgtctc ccgtcgacgt tttataagcg
      421 gcttaatgcc ggtgatcgta aaggtgcctg tgagtcgatt cgctggtgga ttaaggacgg
      481 tgggcgtgat tgccgcacac gttcaaataa ctgctacgga caggttattc gtcgtgacca
      541 ggaaagcgca ttagcttgtt gggggataga tcagtgagaa gagtaaccgc gattatttac
      601 gctctggtta tctgcatcat cgtctgtctg ttatgggctg ttaatcatta ccgcgataac
      661 gccatcacct acaaagcgca gcgcgataaa aaagccagag agctggagct ggcaaacgca
      721 accattactg acatgcagca gcgccagcgt gatgttgctg cgcttgatgc cagatactca
      781 agggaattag ccgatgcgag agctgaaaat gaaactctgc gcgctgatgt tgccgctggt
      841 cgtaagcgcc tgcggatcaa cgccacctgt ccaggctccg tgcgtgaagc ccccaccacc
      901 tccggcgtgg ataatgcaac cggcccccga ctggcagaca cagttgaacg ggattatttc
      961 accctcagag agcggttgat gatgatgcag aagcagctgg aaggggcaca ggaatatatc
     1021 cgcactcagt gcctgaaata agttttgctg atgcgccgta tcgtcgccgt attcccgtat
     1081 taacagcgac tgcagccctg acgggtacct cctgcgtgag tgtgcgggga taatcaaaaa
     1141 cgatacacac cgggtttccc tcatttttca ggagacgtga gcatttaccc gcgaagccgc
     1201 ctgtccggtg cggtggtgga agaaaccgga taaaacaacc tcattgtaca aatatcgatc
     1261 aagtatggcg ctgctgtgtg aattctgaaa aatcacagcg gtcattatgc gtcagttttt
     1321 aacagaggac gtcagaaagt gacatggcaa agctggactg gaaaaagctg gagcaggcat
     1381 tccgacgcga acatgccgaa acgggaataa cattactgga ctggtgccgg aagaaaaaga
     1441 ttaattacaa caccgccaga acccgtataa aaatgggcaa aatcgatcat gaaattgatc
     1501 ataaaaccga tcatgaaatc gatcatgaca tctcagatga agaacccagc aatgacgcgt
     1561 gttccgacga tgaaaaatgc gcaaaaaact ctgaaaaaaa ctgcgcaaat tcggcagaaa
     1621 cgaaacggat tcgtggttcc cgacttttac ctccttcaaa cgctttttct cagcgaaaca
     1681 cccacgccgt aagacaccgt ggatatgcga agtatcttga ggcagataac ctcatggatg
     1741 atgcgtccga catggtgctg ttcgatgaac tggtgttcac ccgggcccgc gcactttcag
     1801 taactaaggc acttaaaggg atgttcgccg acctggaaga ggcaactgac gtggaaa
//
LOCUS       DAZKHK010000324         1824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKHK010000324 DAZKHK010000000
VERSION     DAZKHK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.326"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(131..1282)
                     /locus_tag="ACHOS5_005340"
     CDS             complement(131..1282)
                     /locus_tag="ACHOS5_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9779975.1"
                     /translation="MDKSLSRSVISVVIGGILFGVSAGVLAQDAAENKLIASGSIPEH
                     TDLSASARADGEIALAVGTGASADKKFATSVGSWSAARGQQSVALGGATYAYADASTA
                     LGSVAFVDSTATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNE
                     VYIGYEAESGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDNVEQLAQDTNTRLVVEA
                     KKFREYTDSRTTVGVNSDGTLTRAEGASKTLSVNDGLVALSGRTDRIDATVGSVDRRV
                     TKNTQAIQSTSRQLQEHSTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFN
                     ATAAVGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
     gene            complement(1658..>1824)
                     /locus_tag="ACHOS5_005341"
     CDS             complement(1658..>1824)
                     /locus_tag="ACHOS5_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9779976.1"
                     /translation="VTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEI
                     LAYRVGWAQE"
BASE COUNT          461 a          507 c          352 g          504 t
ORIGIN      
        1 ccatactcca gataacggtt gcgggtcgcc agaatcactt tcatcgctgc cacctgtttc
       61 atgctcacat gagaaaaaac ggcaggcaaa actccacgcc tgccgtcatg cttcatgtca
      121 ctgtcagcaa ttaaaactca aagttcacac cagcgttcca ggatacatcg ccatcgctcg
      181 ctgccacacc tgctttcgct gctgtctgct cattgaaacg gtaaccaaca cccacggcaa
      241 cagcctgttt gtcactgtaa ccaccaacgg cagcagtggc gttgaattta cccacactgt
      301 acggctggaa aagacctgcc agcgctgcgc tctgggctgc cgcgcgtttc atctcttcgt
      361 ggttttcatt aatctgacgc tgatgactgt tcagacgtgt gctgtgctcc tgcaactgac
      421 gggaagtgga ctgaatcgcc tgtgtatttt tagtaacccg gcggtcaaca ctaccaacag
      481 tagcatcaat acggtctgtt ctgccggaca aagctacaag cccatcatta acagacaggg
      541 ttttgctggc tccttctgca cgggtcaggg ttccgtcgga gttaacacca actgtcgttc
      601 tggagtctgt atattcacga aactttttcg cttcgaccac aaggcgggta ttcgtatcct
      661 gagccagttg ctcaacattg tcttttacac ggtcaacctg acgaactgtt gctgcatctg
      721 agggacgagt accgtcactc agaccaccaa gcacccgggt tttataagcc ttacccgact
      781 ctgcttcata accgatataa acctcattgt cacgggttgt gcgtgaatta gctcctaatg
      841 caacactgtt tttatttgtt actgttgcct ttgcccctaa cgcagtacct tctgttgcat
      901 ctttatcaac tttggctcgg ttacctgctg ccgtgccata tgtagccgtg ctatcaacaa
      961 atgcaacgct tcccagggct gtagatgcat ctgcataagc atatgttgcc ccacctaaag
     1021 ccacactttg ctgtcctcgt gctgcagacc atgatcccac tgatgttgca aattttttat
     1081 ccgcgctggc ccctgtacca acagctagcg caatctctcc gtccgcgcga gctgaagcag
     1141 aaagatctgt atgttctggg atactccctg aggcgataag cttattctcc gctgcatcct
     1201 gagccagaac gccagcgctt acaccaaata aaataccgcc aataacaacg gaaataacac
     1261 ttcgtgataa agacttatcc atgtatgatt cctcatgaat ttatttgtat acaaacaaac
     1321 cgaaatacat cagatgaata aagaaaatct gatgtatttc ggcacccatg aggatattcc
     1381 taaaaaaccc cacaaattta attttaatct tttaggaaat atcttagtgt attttttaca
     1441 cccttactct aaatactgga gcacaaggca gagcaatgct caataaaaaa ctgatctcat
     1501 gccgctggat atttcacatc tgcttacact gtaatttctg agcaacatca caatatcatc
     1561 aaaatttaac tgtttagttc atcaatgata tttccctgcg taattattac gactctttca
     1621 gtaaaaacag aaagggacaa tccctctttt ccaccaatta ctcctgtgcc cagccaaccc
     1681 tgtatgccag tatctcatcc gcactggtca gcttttccag ctccttcttc atggtgcgct
     1741 gccggacgtg gatttccatc cctttggtga acatcgcctg ctctgccgct tcactcagcg
     1801 ctataagctc ttctgctgtc accc
//
LOCUS       DAZKHK010000325         1805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKHK010000325 DAZKHK010000000
VERSION     DAZKHK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.327"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..358
                     /locus_tag="ACHOS5_005342"
     CDS             <1..358
                     /locus_tag="ACHOS5_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779977.1"
                     /translation="GSVTNNNEGSFNGTNIEDGNKKSDLGIKKEKKQMDKNMNKHGRL
                     RSVTVSEKKFLDDAVCEAMKVKGKNLTKEERRKVLGVAREQICSQRKANQIKSERTKA
                     RHAAVFEWKRPDSFRR"
     gene            569..>1805
                     /locus_tag="ACHOS5_005343"
     CDS             569..>1805
                     /locus_tag="ACHOS5_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779978.1"
                     /translation="MSHGNFKIKRIAATSILCFSVLYSNTAFAVWPVTEEVLTPYVMG
                     ASPQSMGNGMIETLVSMNDKLAQANITANENQKNSVISNANQAIQEQRMQNILKRIPD
                     ANACEEATAAGGRGSAGSNTSSAKAALAENSVKMFTGASTGINEAKNNVMGRVSIEVC
                     SDEDVKYNRGGCSAIGAYADKDKSATSLTTPPLSISDAQGNKVSNQSYDVKQMKVADA
                     AKRNLIGNLPLQQLNDRNLEDTKEGREYLSAFSSFVARSTAGTNAVDSILAIKKSGDV
                     SVNSRGVNIGSGLGSTAGASQAWTDASVKTKYATLFPGAEFPSKPSEWEMLRYEIYSR
                     YADTTGADAWQVKISSTDEKETAHEQARMQAIDLRLQMLLIERTEDTNILLAALLGQQ
                     LQPVDKVMLNNLKINATKST"
BASE COUNT          643 a          293 c          403 g          466 t
ORIGIN      
        1 aggttctgta acaaataata acgagggaag ttttaatgga accaatattg aagatggaaa
       61 caaaaaatca gatctcggga ttaaaaaaga gaaaaaacaa atggataaaa atatgaataa
      121 gcatggacgt ttaagaagcg tgacagtatc ggaaaagaaa tttcttgatg atgcagtgtg
      181 tgaggcaatg aaagtgaaag gtaagaatct taccaaagaa gagagaagaa aagttctggg
      241 tgttgctcgt gaacaaatat gttctcagcg taaagctaat cagattaaaa gtgaaagaac
      301 taaagcaaga catgctgcag tgtttgaatg gaaacgccct gattcttttc gtcgctaaaa
      361 aactccatcc gaatttgttt aaatattata aatcgacatg gcaattctct taatgatgat
      421 tcaggagtga atatattaat ctgagttggt tttaaaatct atgcttttta aacacgataa
      481 gggatcatta agcatagtca gaacaattga caattcattt ttaatttatt aattaataaa
      541 gaatgtaatg aattaaaggg agtgtgtgat gagtcatggt aatttcaaaa taaaaagaat
      601 agcagcgact tcgattttgt gtttttctgt tctgtattca aatacagctt ttgctgtctg
      661 gcccgtcacg gaagaagtat tgacacctta cgtaatgggg gcatcccctc aatctatggg
      721 aaatggaatg attgagacat tagtctcaat gaatgacaaa cttgcacagg ctaatattac
      781 tgctaatgag aatcaaaaga actctgttat aagcaatgca aaccaagcta ttcaggagca
      841 aagaatgcaa aatatcctta agcgaattcc tgatgctaat gcttgtgagg aagctacagc
      901 agcaggaggt agagggtcgg caggatcaaa cacaagctca gctaaagctg ctttagctga
      961 aaatagtgtg aaaatgttta cgggagcaag caccggtata aatgaggcta aaaataacgt
     1021 gatgggccga gtctcaattg aagtctgttc tgacgaggat gtgaaataca acagaggtgg
     1081 atgtagtgct attggtgcat atgctgataa agataaatct gctacctcac tcaccacacc
     1141 accgttatcc atatctgatg ctcaggggaa taaagtttca aaccaatcat atgacgttaa
     1201 acagatgaaa gtggctgacg ctgctaagcg aaatctaatc ggtaatttac cgctacagca
     1261 gctaaatgac aggaatcttg aagatacgaa agaaggacgc gaatacctgt cagcattctc
     1321 aagttttgtt gccagaagca cagcgggaac taatgctgtt gattctattc tggcaataaa
     1381 gaaatctggt gatgtatcag taaactcaag aggagtaaac ataggctctg gacttggttc
     1441 tacagcagga gcaagtcagg cgtggactga tgcgtctgtg aaaaccaagt atgcgacatt
     1501 attccccgga gcagagttcc cgtctaaacc ttcggaatgg gaaatgttga gatatgagat
     1561 ctattctcgt tatgcggata ctactggtgc tgatgcatgg caggtcaaga tcagcagtac
     1621 agatgaaaaa gaaaccgcac atgagcaggc aagaatgcag gccattgatt taagattaca
     1681 gatgcttcta atagaaagaa cagaagacac aaatatttta ttagcagctc tattaggaca
     1741 gcaactacaa cctgttgata aggtgatgtt gaataacctg aaaatcaatg cgacaaaatc
     1801 aacac
//
LOCUS       DAZKHK010000326         1800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKHK010000326 DAZKHK010000000
VERSION     DAZKHK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.328"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1660)
                     /gene="vat"
                     /locus_tag="ACHOS5_005344"
     CDS             complement(<1..1660)
                     /gene="vat"
                     /locus_tag="ACHOS5_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vacuolating autotransporter toxin Vat"
                     /protein_id="HFP9779979.1"
                     /translation="MNKIYALKYCYITNTVKVVSELARRVCKGSTRRGKRLSVLTSLA
                     LSALLPTVAGASTVGGNNPYQTYRDFAENKGQFQAGATNIPIFNNKGELVGHLDKAPM
                     VDFSSVNVSSNPGVATLINPQYIASVKHNKGYQSVSFGDGQNSYHIVDRNEHSSSDLH
                     TPRLDKLVTEVAPATVTSSSTADILNPSKYSAFYRAGSGSQYIQDSQGKRHWVTGGYG
                     YLTGGILPTSFFYHGSDGIQLYMGGNIHDHSILPSFGEAGDSGSPLFGWNTAKGQWEL
                     VGVYSGVGGGTNLIYSLIPQSFLSQIYSEDNDAPVFFNASSGAPLQWKFDSSTGTGSL
                     KQGSDEYAMHGQKGSDLNAGKNLTFLGHNGQIDLENSVTQGAGSLTFTDDYTVTTSNG
                     STWTGAGIIVDKDASVNWQVNGVKGDNLHKIGEGTLVVQGTGVNEGGLKVGDGTVVLN
                     QQADSSGHVQAFSSVNIASGRPTVVLADNQQVNPDNISWGYRGGVLDVNGNDLTFHKL
                     NAADYGATLGNSSDKTANITLDYQTHPADVKVNEWSSSNRGTVGS"
BASE COUNT          461 a          437 c          386 g          516 t
ORIGIN      
        1 atgaacctac tgttcccctg tttgatgatg accattcatt aacttttacg tctgccggat
       61 gcgtctgata atccagagtg atattagccg ttttatcact gctgttaccg agagttgcgc
      121 cataatcggc ggcattcagc ttatgaaatg tcaggtcatt cccgttaaca tccagaacac
      181 ccccccggta gccccaggat atattgtccg gattaacctg ctggttgtct gccagcacga
      241 ctgtcgggcg gccgctggca atattcacgc tactgaatgc ctgaacgtgt cctgaactgt
      301 cagcctgctg attgaggaca acggtcccat ccccgacttt caggccgccc tcattaacac
      361 cggttccctg tacaaccagg gttccttcgc cgattttatg caggttgtca cctttcacac
      421 cattaacctg ccagtttacg gaggcatcct tgtccacaat aataccggcc ccggtccagg
      481 tacttccgtt tgaagtggtg acagtgtagt catcagtaaa tgtcagtgaa ccggcaccct
      541 gcgtgacaga gttttccagg tcaatctgac cattatgtcc caggaatgtc agatttttac
      601 ctgcgttcag gtcagaacct ttttgcccgt gcatggcata ttcatcggaa ccctgtttca
      661 gagagccagt gccggtgctg ctgtcaaatt tccattgcag gggggcgccg gatgaggcat
      721 taaaaaagac gggagcgtca ttatcctctg aatagatctg tgagagaaaa ctctgaggaa
      781 taagagaata tatcaaattg gtcccccctc ctactcccga gtaaacaccg accagttccc
      841 actgcccttt ggccgtattc cagccaaata atggagaacc actgtcgccg gcctctccaa
      901 aagagggcag gatgctatga tcatgtatgt tgccccccat atacaactga atgccgtctg
      961 agccgtgata aaagaatgat gtcgggagta ttcctcctgt cagataacca tacccacctg
     1021 ttacccaatg tcgcttaccc tgactatcct gaatatactg acttcccgaa ccagccctgt
     1081 agaatgccga gtattttgaa gggttcaata tatcagctgt tgatgagctg gttacggtag
     1141 ccggagcaac ctcagttacg agcttatcaa gtcttggtgt gtggagatca gatgaactgt
     1201 gttcattacg atccacaata tggtaactgt tctgaccatc accgaagctg acgctctgat
     1261 atcctttatt atgttttaca ctggctatat attgcgggtt aattaatgtt gcaacgccgg
     1321 gatttgagct tacattcaca ctgctaaaat caaccatggg cgctttatca agatgtccta
     1381 ctaattcccc tttattatta aaaataggaa tgtttgttgc gccagcctga aactgccctt
     1441 tgttttctgc aaagtcgcgg tatgtctggt aaggattgtt gccaccaacc gttgatgcac
     1501 cagcaacggt tgggagtaat gcagatagtg ccagagaggt aagtactgaa agtctttttc
     1561 ctctgcgggt actcccttta catacccttc gggctagttc agagacaacc tttactgtgt
     1621 tagtaatata acaatatttt agagcgtata ttttattcat agtaaatgat tccggaacgt
     1681 atattccaaa gttacattaa taaccataga caatttcaaa cgaaagcatt gttgaattgc
     1741 gcgctaatta ttaattagct atataattat aaatgtgaca tatgtcacat ttatttttgc
//
LOCUS       DAZKHK010000327         1787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKHK010000327 DAZKHK010000000
VERSION     DAZKHK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.329"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(168..1355)
                     /locus_tag="ACHOS5_005345"
     CDS             complement(168..1355)
                     /locus_tag="ACHOS5_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFP9779980.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLADMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTPDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAVEGNSGR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGCQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1355..1720)
                     /locus_tag="ACHOS5_005346"
     CDS             complement(1355..1720)
                     /locus_tag="ACHOS5_005346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779981.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
BASE COUNT          429 a          528 c          421 g          409 t
ORIGIN      
        1 caaaagatta aaaaaacacc aaaaaaacgg atgtttattc aacactgcct gcgtgccatg
       61 agtgcaccgc catcaattaa atggatctta tcgattgata ctgtatatca ctgatggttt
      121 tttactccat tcccctgaaa atggacgcaa cttccccgat gcggctctca gccgcacacc
      181 cgctgttgcg ccagtggctc atgcatcagg accagctctg tcagacggta gccccgcttc
      241 agccccgtaa aacgcatctg acatccgcac aggatgcact tcagcgggtc aaccttcagt
      301 aaccgctgat acatccctct ccacctgatt tgcatcgccg tctttctcac cgtctccgtt
      361 atgacgtaca caacatcttc cagtaaccgg cgcttcaccg gactcaggaa accgtagtac
      421 ctcaccatac ggaacccctt atccgccaca tgccaggaga acctttccat aaactcatca
      481 ccactcatca ccaggtattc ttcccgtttt gtccggtgac tgttgtaacg cagaccaatt
      541 tcatcctgac cagcatagtg ctccagacgg ctcatcggca ccggcggttt tttcaggtaa
      601 gagccgaagt acaccgccac atgtgtggcg ttatccatca cccgggatac gttgacattc
      661 cagccccgcc ggtaatgact gtccaggaag cgattccagt cccgtccgct gtttccttca
      721 actgccagcg catccggtat caccaggtca gggtatttcc gtgacagcaa ccgtgttatc
      781 cggtaacgcc acatgctcat caccttacgg gcgtaaaaat gaaggttttt ccaggtgtgg
      841 tctggtgtca cgccgccggc cgttgtcgac aggtgaatgt gcggatgcca ctgctggtca
      901 cgtccccatg tgtggatcac cgtgaatatc cccggcacca catctgcctg gcggcagatt
      961 tcctgtatca catccgcagc aatgcggctc atatctgcca gtaaccaccg gttgtggaac
     1021 accagggacc agtactggca gggaagtgtg aacacaatat gctgccacgg acagtcggga
     1081 accagactca gcagatactg tatccactgt gcgccagcct tcactccgca gtgcgggcag
     1141 gagcgacttt tacaccggaa gcagaccttt tttgtgtggc aacagtccgg agatgaacag
     1201 caccactgtg tgtaccccat cagcgtggtt ccgcacgcca tgatttttgt caccgactca
     1261 atgaccaccg gacgcactga accttccggt tgtttctcca gccagttaag ccagcggttt
     1321 ccctgctgaa aaatgtcggc aaaacgggga agcatcagaa gggcgggggg actccgtccg
     1381 gccagtgaac tgtgccacac tccgggcagt acatgccgcc ggcgctgata ccggcaagaa
     1441 tggtcgcaaa ctcccgctcc gtgcagcggg ctatttcagg atacccttcg tcatcaacac
     1501 gaacaaacca gaagaccagc tttttgtttc cggcatccac aaagaaggga atattcaggt
     1561 ctgcgcagca ctcaacggca tcgtcaaaat tatcaaagcg cagagcttct gcatcttctt
     1621 cgtcaaagaa gtcatcgtcg tgaagcttca cgacatagcg gggaagtttg cttctttgag
     1681 gggcaggttt tcgtttacgg ggtttagctg aacgggccat ataaccaact gaaagacaat
     1741 gatattgtct gtttttataa cggtaagctc agaccatgac aagccgc
//
LOCUS       DAZKHK010000328         1785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKHK010000328 DAZKHK010000000
VERSION     DAZKHK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.330"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..577
                     /locus_tag="ACHOS5_005347"
     CDS             44..577
                     /locus_tag="ACHOS5_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9779982.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVVPGMKLSKEKCDQVNAIERDKVLAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            868..999
                     /locus_tag="ACHOS5_005348"
     CDS             868..999
                     /locus_tag="ACHOS5_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779983.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            963..1117
                     /locus_tag="ACHOS5_005349"
                     /pseudo
     CDS             963..1117
                     /locus_tag="ACHOS5_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1182..1643
                     /locus_tag="ACHOS5_005350"
     CDS             1182..1643
                     /locus_tag="ACHOS5_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9779984.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
BASE COUNT          432 a          398 c          512 g          443 t
ORIGIN      
        1 attaaagaag accggcgtaa ggcggcgcgg ggagagtaag ctgatgagca ggaaattccg
       61 ctatggttta tcagcggtcg ttctggcgct gattgctgca ggtgcttctg cgcctgaaat
      121 cctcgaccag tttcttgacg aaaaagaagg taaccacacc acggcatacc gtgatggtgc
      181 gggtatctgg accatctgcc gtggtgccac ccaggtggat ggtaagcctg tcgtccccgg
      241 catgaagttg tcgaaggaaa aatgcgacca ggttaacgcc attgaacgtg ataaggtgct
      301 ggcatgggtg gcgaaaaaca tcagagtgcc actgaccgaa cctcagaaag cggggatcgc
      361 gtcattctgt ccttacaaca ttggccccgg taagtgtttc ccgtcgacgt tttataaacg
      421 aattaatgca ggcgatcgca ggggagcgtg tgaagcgatt cgctggtgga ttaaggacgg
      481 tggcagagac tgccgtatcc gctcaaataa ttgctacggt caggtatccc gtcgcgacca
      541 ggagagtgcg ctggcatgtt ggggtatcga caggtagcag aatattttgc tgaaaaataa
      601 cgttggtcaa cgcggacgga taacacgaaa tcctgcgaac tggcaaaacc taagtgaata
      661 aaagtaaaaa ccccgttttt ggcagcaagc ggggttttgt gtttatggca gtaagctatg
      721 ggtggctgcc ttgattgatt ttagcaaact gattagggag ttgcgactca tgattagtca
      781 attaccaaac tggaagtttt tgctggtctg gagcatccct tttttatggg gggtatccca
      841 gttaattgtg gcaattaagg ggtagctatg tcagacaaac tcataacgct ggcgaagatc
      901 ctctgtgtaa tcgtcggcat ttcattttca ctaatgctgg ttgctatttt tcttttcctg
      961 ccctggatga tgttgtcttc gtcggggatg ctggggtaac agtgactgat gacatcagca
     1021 gagcgctggc tttgctatta agtgggtggc ggttggtatc gctgtgtctc cgatactgta
     1081 tgggctggca aaactggtca ttgcgctgaa atcgtgaact ttatttaaac agatgagtaa
     1141 tgaaattgct gtactggtcc gggcaattcg ctggtggtga tatgaaccgt attctgtatg
     1201 tggtgattat tgtactgctg gtggcctgtg gtgcgcttag tctggggctg aatcattacc
     1261 gcgataacgc catcacctac aaagcgcagc gcgataaaaa agccagagag ctggagctag
     1321 caaacgcaac cattactgac atgcagcagc gccagcgtga tgttgctgcg cttgatgcca
     1381 gatactcaag ggaattagcc gatgcgagag ctgaaaatga aactctgcgc gctgatgttg
     1441 ccgctggtcg taagcgcctg cggatcaacg ccacctgctc cggtaccgtg cgtgaagccc
     1501 ccaccacctc cggcgtggat aatgcaaccg ccccccgact ggcagacaca gttgaacggg
     1561 attatttcac tctcagagag cggttgatgc tgatgcagaa gcagctggaa ggggcacagg
     1621 actatatccg cactcagtgc tgaactaagt tttgctgatg cgccgtatcg tcgccgtatt
     1681 cccgcataaa cagagaccgc agccacctta tctgcgtgag tgtgcggggg taatcaaaaa
     1741 cgatgcacac cgggttttct catttttcac gagatgggag cgatt
//
LOCUS       DAZKHK010000329         1785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKHK010000329 DAZKHK010000000
VERSION     DAZKHK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.331"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..431
                     /locus_tag="ACHOS5_005351"
     CDS             <1..431
                     /locus_tag="ACHOS5_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9779985.1"
                     /translation="QVCRMYNNAFVGPERNNHGHAVILKLRELYPTRYIYNEQHLDQA
                     YDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWSGTLSEMNTYVYDAKGSMNAQ
                     EGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWMAH"
     gene            431..>1785
                     /locus_tag="ACHOS5_005352"
     CDS             431..>1785
                     /locus_tag="ACHOS5_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9779986.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQALAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     Q"
BASE COUNT          453 a          407 c          509 g          416 t
ORIGIN      
        1 cgcaggtctg tcgtatgtac aacaacgcgt ttgtggggcc ggagcgtaac aatcacggac
       61 atgccgttat tctgaaactc agggagctct atccgacgcg ttatatctat aacgaacagc
      121 accttgatca ggcatatgat gacgatacgc cccgtcttgg ctggctgaca acccgtcaga
      181 gcaaacctgt tctgaccgaa ggaatgaaaa cgctcctgaa taatggaata tcagggatcc
      241 gctggtcagg cacattatcg gaaatgaaca cctacgttta tgacgcgaaa ggctccatga
      301 atgcacagga aggctgtttt gatgatcaac tcatgagcta catgattgcc caggagatgc
      361 gcgccagaat gcctgtgagg gtaaaacaga aaacggataa acgcagaact acacactgga
      421 tggctcactg atgaaaaatg aaactaacac catggcgacg aaaaacgaca atggagccac
      481 gccgcgtttt tctcagcgcc agttacaggc gctttgttct gatattgaca gccagcctaa
      541 atggcgtgat gccgcaaaca aggcctgtgc gtattacgat ggcgatcagt tgccaccgga
      601 agttcttcag gtactgaaag atcgcggtca gccgatgact atccataacc tcatcgcgcc
      661 taccgtcgat ggcgttctgg gaatggaggc caaaacacgt actgatctgg tggtgatgtc
      721 agacgagcca gatgatgaaa ctgaaaaact ggctgaagct attaatgctg aatttgccga
      781 tgcatgccgc cttggcaata tgaataaagc ccgctctgat gcctatgcgg aacaaatcaa
      841 ggcgggcctc agttgggtgg aggtcagacg aaacagcgat ccgttcgggc ctgaatttaa
      901 ggtgtctact gtcagccgga atgaggtttt ctgggactgg ctgagccggg aggctgattt
      961 aagtgactgc cgctggctga tgcgtcgccg ctggatggat accgatgagg caaaagctac
     1021 attcccggga atggctcagg ttatcgatta tgccattgat gactggcgtg gttttgtcga
     1081 taccacggtt actgaaggcc agcccagtcc gttgatgagt gcatgggaag agtatcagtc
     1141 atgggatcga cagcagaacg aatggcttca gcgtgaacgc cgtcgtgtgc tgcttcaggt
     1201 ggtttattac cgtacattcg agcgtcttcc ggtgattgaa ctcagtaatg gacgggtggt
     1261 ggcctttgat aaaaataatc tgatgcaggc gctagctgtg gcatccgggc gggttcaggt
     1321 gaaagtcggg cgggtaagcc gtattcgtga agcctggttt gtcgggccac actttattgt
     1381 ggatcgcccc tgtagtgctc cgcaggggat gtttccgctg gttcctttct ggggataccg
     1441 aaaggataaa accggggagc catacgggct aatttcccgc gccattccgg cacaggatga
     1501 ggtgaatttt cgtcgtatca agctgacctg gttgcttcag gccaaacgcg tgattatgga
     1561 cgaggatgcc acccagttgt cagacaacga cctgatggag cagatcgaac gtccggatgg
     1621 cattattaaa ctgaatccgg cccgaaaaaa tcagaaaagt gttgcagatg tttttcgggt
     1681 tgagcaggat tttcaggttg ccagccagca gtttcaggtt atgcaggaat cggaaaaact
     1741 tatccaggat accatggggg tttattccgc atttctcggg cagga
//
LOCUS       DAZKHK010000330         1776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKHK010000330 DAZKHK010000000
VERSION     DAZKHK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.332"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..205)
                     /locus_tag="ACHOS5_005353"
     CDS             complement(<1..205)
                     /locus_tag="ACHOS5_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFP9779987.1"
                     /translation="MNLPIYIVSLKRDIERRNKINDVFYRLNINFDFFDAIDAKDPQN
                     KEIIDKMRLSGVGAEMTDGEIACT"
     gene            complement(237..1265)
                     /locus_tag="ACHOS5_005354"
     CDS             complement(237..1265)
                     /locus_tag="ACHOS5_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFP9779988.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFSHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKSRHLKRNGKIASSFYYFMLYYSKK
                     IARKHFSK"
     gene            complement(1291..>1776)
                     /gene="rfaY"
                     /locus_tag="ACHOS5_005355"
     CDS             complement(1291..>1776)
                     /gene="rfaY"
                     /locus_tag="ACHOS5_005355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFP9779989.1"
                     /translation="ERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFN
                     YASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKD
                     GVRIIDLSGKSCTAERKAHDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGK
                     A"
BASE COUNT          563 a          359 c          291 g          563 t
ORIGIN      
        1 atgtacaagc aatttctcca tcagtcattt ctgcgccgac accacttaat ctcattttat
       61 caataatttc tttattttga gggtctttag catctatggc atcaaaaaaa tcaaaattaa
      121 tatttaatcg ataaaaaaca tcatttattt tatttcttcg ctcaatatct cttttaagtg
      181 agacaatata aatgggcaag ttcattaaaa aatctctgga tgattaatct taataattat
      241 ttggaaaaat gcttacgcgc tattttctta ctataataga gcatgaaata gtaaaatgaa
      301 gatgcaattt taccatttct tttcaaatgt ctggatttca cttgatactg cttttcattc
      361 gtggctggaa taaacgccac atcattccag caagaagcct gataagcctc attaaaatac
      421 ttctggcagg gataattcgt ccagtcgttc cagggcttcg ttacgccaac ataatgaatc
      481 agcttagtat catcggtaat ggtcttttta taatcttcat cgcttttatt ttttaactca
      541 tagtcaatgc cataaaagca atcataatcg ccgctaataa aaatacaatg actaaagaac
      601 agaatattta aaatatcctg atcaaggcat ttatatttcg tgtgctgcgg gtcacttgcc
      661 agcatagcaa tggctttatt cataaaatca tttttcgccc atgcttcaag ctgcagatat
      721 actacaccgg cgttgaaata attccctgga agtccctcta tagccagacg cttcgcaggt
      781 ttttcctgca taaaaggcac atctttaata acagccgcga acttatcgcc atcaaaatta
      841 atatcaagca atccagctaa tgagccttta catatgacat cagcatcgat atataacaga
      901 ttttcaatag tttcactcag atactggaac gcaactaaac ggaagaatgt tgcataagac
      961 cactgtttca ggctggggaa gatatcaata aaggcactgg atactttata aattcttact
     1021 tcaacgttag catttttgct ggtaaaagca ttaacacgtt gtagataatc ttcatcaatg
     1081 tagtcagtaa agagatgaaa cttaaccgca aggtccgggt tatgcataac aactgattgc
     1141 atagatactg ccgcaccaaa taaaaagttg cggtcaatac cgtaagcgat atttaacaca
     1201 cgatcggtgt tagcaatttc cccagagaca gcgacttcag taatactctc agctaataaa
     1261 tccatgttgg ttccgttttg actgtatggt ttacgctttg ccttttaatt ttttaataaa
     1321 tttgcgtagt ttggttctgt agattactga atagtagcca taatctttta tttcgttggc
     1381 aatacccagg tgacgttcca tggccagacg atcatgggct ttccgctctg ccgtacagct
     1441 tttaccggat aggtcaatta tcctgacacc atctttacta acgataaagt taccccggtg
     1501 tgggtcgccc gatagcatat ttaaggcatg caatttttcc attgaagcct taatttctgc
     1561 tttaacatct tctggaataa tcggcatatc attgagctct acgccctcga catactcgat
     1621 aagcatgata aaaacactgg catagttgaa aattttacgc tcagccagaa aataaaaatc
     1681 attaggaaaa gtaagacctg cacttctgac acgatcggtt tcaacgatca aattctgata
     1741 gtagtcacct tttacaaaag attttaaaaa acgttc
//
LOCUS       DAZKHK010000331         1774 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKHK010000331 DAZKHK010000000
VERSION     DAZKHK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.333"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..75
                     /locus_tag="ACHOS5_005356"
     CDS             <1..75
                     /locus_tag="ACHOS5_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9779990.1"
                     /translation="DYSKNGAGIENYNFITTAGLKYTF"
     gene            262..1746
                     /locus_tag="ACHOS5_005357"
     CDS             262..1746
                     /locus_tag="ACHOS5_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9779991.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT          569 a          391 c          341 g          473 t
ORIGIN      
        1 gactacagca aaaatggtgc aggcatagaa aactataact tcatcactac tgctggtctt
       61 aagtacacct tttaacgaag ttaaccagat tttctccccg gtggtattgt ataaccccgg
      121 ggaatttgtt tacagttaca agtaggcaat atccactaat tttgtaagca tttaacctat
      181 gtcataacgt gtaatctctt accgcaacac aagacctcaa tatattcaaa tcttatcttt
      241 attccgatat tcagagacac aatgcgaaaa ataattactc atttcaaagt tgttttaacg
      301 ttacttctac cagtaaccgt atctgcccag cagatacagt ggcaatcctg tatggccagt
      361 caattcaacc actggtttgg tgaggaaaaa ccgtctcctg acttactatg tggttatttg
      421 tctgttccat taaaatatac agacacaggc ggagatgctt cttatgaaaa aaaatcacaa
      481 gtcaaactag cgttgacaaa attgccggca aaaagcaagc ataaaggaag tatcctgata
      541 ataagtggtg gtcccgggtt accaggcata aatccttata ttaactttga ctggccagtc
      601 acaaatcttc gtgagtcatg ggatattatt ggatttgatc ctcgaggcgt cggacagtcc
      661 actccgacaa taaactgccg gcaatcagat acagagactc aggaaaacat aaccgaaaag
      721 caacaagtat taaataaaat taatgcctgt atccataata ccggagccga agtcattcgc
      781 catatcggct ctaacgaggc tgtatacgat attgatcgta ttaggcaagc cttgggggat
      841 aaacaactga cagccgtggc gtattcgtat ggaactcaaa ttgcagcctt atatgcagaa
      901 cgttttccct acaacgtaag atctatcgtt cttgatggag tcgtcgatat cgatgacctg
      961 gaggacaact tcacatggca actcaaacag gcacagagtt atcaggaaac gtttgatcgc
     1021 tttgcatcct ggtgtgcgcg tacaaaaagt tgcccgcttt cttcagacag agataaggca
     1081 ataactcagt tccatgagct attatcaaaa ttacatcaca aacctttatt agacagtaag
     1141 agagaaaata tatcttcaga tgaactcata tcattaacaa cagaccttct gctatggcgt
     1201 tcatcatggc caacccttgc aactgccata cgccagttct ctcaggggat tgtcagtaat
     1261 gaaattgaaa ctgcgctcag tgctccgata gcctcagaag agtcaagcga tgcttcgggg
     1321 gtaatcctct gtgtagatca gggggatgag caattaacac cagaagagcg aaaatcccga
     1381 aaagacgctc ttgcgaatgc cttcccggct attaactttg acaatggacg ttccgattca
     1441 cctgattttt gtgaattatg gccaatacat agcgacctga acaaaactcg cctgaaaaat
     1501 actgttctgc cctctggttt actgtttata gcacacaaat acgacccaac aacgccctgg
     1561 attaatgccc gtaagatggc agagaaattt tccagcccgt tactaacaat aaatggtgat
     1621 gggcatacat tagctctcac cggagttaat ttatgtgtag ataaagcagt tgtacatcac
     1681 ctgatcactc cacaaaaaat agaaaatata tactgcccag gaaattctga agcagaaata
     1741 caataatttc aaaatattcc gctatttgct ccca
//
LOCUS       DAZKHK010000332         1768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKHK010000332 DAZKHK010000000
VERSION     DAZKHK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.334"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..258
                     /locus_tag="ACHOS5_005358"
     CDS             <1..258
                     /locus_tag="ACHOS5_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFP9779992.1"
                     /translation="EVKAEAFSAAKAKLEDAIKAFSAITPEKGERYRRFAARRRDDTD
                     KEEIKAIAEKLNDLTTKLSATGITPRPAGTPAESEKPFDFV"
     gene            270..1289
                     /locus_tag="ACHOS5_005359"
     CDS             270..1289
                     /locus_tag="ACHOS5_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9779993.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGSENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAV
                     PGVGG"
     gene            1307..>1768
                     /gene="gpM"
                     /locus_tag="ACHOS5_005360"
     CDS             1307..>1768
                     /gene="gpM"
                     /locus_tag="ACHOS5_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP9779994.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYITDGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRN"
BASE COUNT          416 a          545 c          510 g          297 t
ORIGIN      
        1 gaggttaagg ctgaagcctt cagcgccgcg aaagcgaagc tggaagacgc gattaaagcg
       61 ttcagtgcca tcacaccgga aaaaggcgaa cgttaccgcc gctttgccgc ccgtcgccgg
      121 gacgacaccg acaaagaaga aatcaaagcc attgccgaaa aacttaacga cctgaccacg
      181 aagctgtcag ccaccggcat cacgccacgc ccggctggca caccggcaga aagcgaaaaa
      241 ccgtttgact ttgtttaagg atacgcaaca tgcaactgaa ccagaaaacc gaatcactgc
      301 ttcgtaagta cgctgccggg ctgggtgcgg caaacggggt tgacgacgtc acacgctatt
      361 ttgcgctgtc tgacccgaag gagaccgcgc tgcgtgacgc catcatgcac tccgacgagt
      421 ttttatcaaa actgccgaac gtgctggatg ttgaacaggt gacaggccag gtggttacca
      481 ccggcgttcc gggcctccat accggacgta aaaaaaatgc ccgcttcacc cttccgctgg
      541 gtgttaccgg gaatgaatac aagctggctg aaaccgattc cggcgcccac ctcctgtaca
      601 ccacactggt cacctgggcg aacgcaggaa gtgaaaacga gttctttaac cgcattcaga
      661 atttcgttaa cagacagatt gcgctggata tcctgcgcat cgcctttaac ggtaccagcg
      721 tggccgaaga taccgacccg gtaaccaaca aaaacggcga ggacgtcaac accggctggc
      781 atgccatcgt caaaggccgc agcgcaaaac agatcgtgga ggctggcccg acgctgggca
      841 agggtggcga ctttatcggg ctggatgcag ccgtggcaga cctcgtgcat accctcattg
      901 atgagtcata tcgcggtcat ccggatctgg tggtactggt tgcgcccgac cttgtggcac
      961 aggacaaggt gacgctgctt aacgccatcg acgcccccac ggaaaaagtg gccgcacagc
     1021 ttatcggtcg ccagatcgcc gggctgacag catacacccc gccgttcatg ccggaagggc
     1081 gcctgattgt caccacgctg gcaaaccttc acatctacac gcagcgcggc acccgtcagc
     1141 gccgtgcgga gtggaacgac gatcgcaagc gttttgaaaa caactatctg cgcatggaag
     1201 gttacgccgt ggagcacgac gaaatttacg cggcctacga caaactgacc atcaagggcg
     1261 cagaagccgt accaggcgtc ggcggctgag gacggagggc ggcgcaatga caccctgcca
     1321 gcggcaccgg gcgaaaatcc gcacacagga ggcactcgaa cgccgggagg cgctgacggc
     1381 ctccccggtc agcttccacc tgttacgcgc ggagcttgat cgcgatgtgg cacggctgcg
     1441 cagtcttccg gtccgcgaag agcggctggc gttcaagcgc gacattctcc tgccgcgctg
     1501 gctgcccgtc gcggagcgct atatcactga cggaaagcgc cacgcctgcc cggtactggt
     1561 gtactgcatc atctggctgt tcgacacggg cgacctgagt cgcgcccttg actgggcaga
     1621 cattgccatc agtgaaggcc aggccacacc ggacaacttc cgcagcaccc tgcccgcctt
     1681 cgtggcagac acggtgctgc agtgggccac cgacaacagc gaggccggac acagcataga
     1741 accgtatttt tcgcgcacct tcaggaac
//
LOCUS       DAZKHK010000333         1748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKHK010000333 DAZKHK010000000
VERSION     DAZKHK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.335"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..763)
                     /locus_tag="ACHOS5_005361"
     CDS             complement(<1..763)
                     /locus_tag="ACHOS5_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP9779995.1"
                     /translation="MNCWEILGLQSGAEERDIKRRYAQLVKNCRPEDDPAAWQQLHDA
                     YEQALRYDDEDQWQEEEEEQPINMSRGIVHPRPVMTFRAPNTFSGHPLPDVTIKSEGE
                     FTWRAVLDMLCEENEATPEHAPMQLAKALSFLEKKDLSSRMCFEETLLLHLHRVFRPL
                     LTLAAAQTFRWHLVAGSRQPVIQTEINESCLLYYRIEKQITPFFASNLSPLEEMENGK
                     EMATIYQSLKDNEESLKWFDIAVLNKVAEFELSDNY"
     gene            complement(791..>1748)
                     /locus_tag="ACHOS5_005362"
     CDS             complement(791..>1748)
                     /locus_tag="ACHOS5_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9779996.1"
                     /translation="AMYYIGEIIKKDFVQAKYWFEKSAGQGNNDALLNLALMYRDGKG
                     VNKNPQKAISLYLNAANKNHPLAQHSLACMYRDGEGVEVDDEQAFKWCQKSAEQGYAE
                     AQYHLATMYIDGRGVDVDYQQVVYWLNLSADQKYSKAECTLGYMYYKGTEIPQDMTMA
                     INLLKSAADHGDIDAALVLGQLYVHGREGYLPKDIHQAIAYLNQAQEGGEEQSAEILG
                     MIYYQGFEEIAENSALSFNMFFKAAYAGIKRAQRMLSIMYMEGDGTDKNYILSWAWGI
                     ISEAYHDLESNDWLREVSKSLSLQDLSRAQRMARILKKRYQL"
BASE COUNT          464 a          412 c          313 g          559 t
ORIGIN      
        1 tatagttgtc tgatagttca aattcagcaa ctttatttaa tactgcgata tcaaaccatt
       61 tcaggctctc ttcattatcc tttaatgatt gataaatcgt agccatttcc ttcccgtttt
      121 ccatttcttc cagtggagac aagttactgg caaagaaagg ggtaatttgt ttttcgatgc
      181 gataataaag aagacaactt tcgttaattt ctgtctggat tacgggttga cgactgcctg
      241 cgactaaatg ccatctgaac gtttgtgctg cagcgagagt taataatggc cggaataccc
      301 gatgaagatg cagtaataac gtttcttcaa agcacattcg tgagctgaga tcttttttct
      361 caaggaaaga taacgcctta gcaagttgca tgggggcgtg ttcgggggtt gcttcattct
      421 cctcacagag catatccagc acggctcgcc aggtgaattc cccctctgat ttaatagtga
      481 catcaggaag tggatgacca gaaaaggtgt tgggtgcgcg aaatgtcatc acaggacgag
      541 gatgaacaat acccctggac atattgatcg gttgctcttc ttcctcttct tgccactgat
      601 cttcgtcgtc ataacgtaaa gcctgttcat aagcatcgtg caattgttgc caggctgcgg
      661 gatcatcttc cgggcggcaa ttttttacca gttgcgcata acgacgctta atatctcgtt
      721 cttccgcacc agattgtaaa cctaatattt cccagcagtt cattatgcct ctcttttccg
      781 tgaaagtaat ttataattga taacgttttt tcagaatcct cgccatccgt tgcgcccggc
      841 ttaaatcctg caacgacaaa cttttgctga cttctcgcaa ccagtcattg ctctccaaat
      901 catgatatgc ttcagaaatt atcccccacg cccatgacaa aatgtaattt ttatctgtgc
      961 catccccttc catatacata atagaaagca ttctttgcgc tcttttaatt ccagcgtacg
     1021 ccgctttaaa aaacatgtta aaacttaaag ctgagttttc tgcaatttct tcaaatccct
     1081 ggtaatagat catacctaat atctcggcgg actgttcctc gcctccttcc tgggcttgat
     1141 ttaaatacgc aatggcctgg tggatatctt taggcaagta tccttccctg ccatgaacat
     1201 aaagttgacc taaaactaag gctgcatcaa tatctccatg atcagcggca gattttaata
     1261 gattaattgc cattgtcata tcttgaggga tctctgttcc tttataatac atatatccca
     1321 acgtacactc tgccttacta tatttctgat ctgctgataa atttagccag taaacaactt
     1381 gttgataatc cacatcaacg ccccgaccgt ctatgtacat tgtggcaagg tgatattgtg
     1441 cttcagcgta cccttgttct gccgattttt gacaccattt aaaggcctgt tcatcgtcaa
     1501 cttccacacc ttcaccatcc cgatacatac atgccaaact atgctgcgcc agaggatgat
     1561 ttttattagc cgcgtttaga tataatgaaa tagctttttg tggattttta tttacacctt
     1621 taccatcacg atacattaac gccaaattta ataacgcatc attattgcct tgccctgcgg
     1681 atttctcgaa ccaatatttt gcctgcacaa agtctttttt gataatctcg ccaatataat
     1741 acattgca
//
LOCUS       DAZKHK010000334         1745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKHK010000334 DAZKHK010000000
VERSION     DAZKHK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.336"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            308..490
                     /locus_tag="ACHOS5_005363"
     CDS             308..490
                     /locus_tag="ACHOS5_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9779997.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            426..800
                     /locus_tag="ACHOS5_005364"
     CDS             426..800
                     /locus_tag="ACHOS5_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9779998.1"
                     /translation="MLRKSAPVSQPEANPKSPLLLNNPFQRENPMSEVKPLVTADAVK
                     DALRSEDVRGALKQKVRQTFEAQIEADVDAILDTILGEQPGEQPEPEPQPEDITSENG
                     DIQPESPVVDVTEAQPEPGTML"
     gene            876..1091
                     /locus_tag="ACHOS5_005365"
     CDS             876..1091
                     /locus_tag="ACHOS5_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9779999.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1096..1320
                     /locus_tag="ACHOS5_005366"
                     /pseudo
     CDS             1096..1320
                     /locus_tag="ACHOS5_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
BASE COUNT          487 a          363 c          519 g          376 t
ORIGIN      
        1 gacgccggta ctggatgaga aacggggtac accgttcaca ctggtgaaat ctccggcatc
       61 aggggcggcg gacaaactgc aactgacaga catatcaaag gcggcggcat atacgctgct
      121 gattgacagt gtgaaggtgg aagtgaacaa cgcggacgtc gcggcatgat aaaaaaagcc
      181 gccagcggca ggaatggaag ctggcggagg taatcccaat ggagaatgta aagaaaagat
      241 gctttcgtac atcggttgtt ttttaaatga aaacagttct cattgtcaac cataacggta
      301 agaaattatg acatttattc atcaggtgat gctgtacttc tgtacggcgg tctgtgtgct
      361 gtatctcctt tcgggtgggt acagggccat gcgtgacttc tggcgcagac agatagacaa
      421 aagggctgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagagcccat
      481 tactccttaa taaccccttt caacgagaaa atcctatgtc agaagtaaaa cctctggtca
      541 ctgctgatgc agtgaaagac gcgctgcgct cagaagatgt gcgtggagca ctgaaacaga
      601 aagtccgcca gacatttgaa gcgcagatag aagcagatgt ggatgcgatt ctggatacga
      661 tactgggaga gcagccaggc gaacagccag agccggaacc gcaaccggaa gatatcacct
      721 cagaaaacgg cgatattcag ccggaatcac cggtggttga tgtgactgaa gcacaacccg
      781 aaccgggcac catgctgtaa gggtgagtca gggccatcag taaagtactg ctggcctttt
      841 tcatgttgtg agcttccgtg tgcgggagac ggggtatgta ccagatggaa aaaatcacaa
      901 caggtgtgtc atacaccacg tcagcggtgg gaacgggcta ctggttcctg cagttgctgg
      961 acagggtttc cccgtctcag tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc
     1021 tgctgacata cctgaccaac ctgtatttca aaatcagaga agaccgtcgt aaggcggcgc
     1081 ggggagagta aggcgatgaa gaaaaaatac gaactggttg ttaaagggat aaataattac
     1141 ccggataaga ttgctgttac tgtggcactg gaaactggtg ggtatccgtc gctgttgttg
     1201 ccaaatgtgg cgattagtct tgaccgtact gaaggtgcca cgctggagtt ttacgaagct
     1261 gaggcgaaaa aacaggcgaa gcagtttttc atggatgcgg ctctcggatt acgcgaaggg
     1321 actgcgcgat gtggctttca gaatatttct tcatggacaa tgccggaaaa aatcgccact
     1381 tacccggggc gtgaaaaggt caacgaaccg gtaaaaaatg cgcctgatga tgtgcagagc
     1441 gcgatggtta atgtggagct tccggcacgt gtgacacaag gagtgaaagt cggggaagat
     1501 tctcgtcttg tgctgaccaa aagaataggc agcgtggtca gtgttcatat tgatcatgag
     1561 gaagttctgt cggttatgta cagggaagac tacgatccga aaacaacatt cagcgactat
     1621 gaagcgagag caaaggagta tgccttgtcg gtatttgctg cggtacgtgg cgaggactgt
     1681 cgtaaaaacg ggcatgaaag tgattttact gtgcctgtgt cggacaaaaa ccaggaacgg
     1741 gagtg
//
LOCUS       DAZKHK010000335         1736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKHK010000335 DAZKHK010000000
VERSION     DAZKHK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.337"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(9..>1736)
                     /locus_tag="ACHOS5_005367"
     CDS             complement(9..>1736)
                     /locus_tag="ACHOS5_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780000.1"
                     /translation="KCAGIWRKNTKKSHALILNLIMFCEIFLSSLSSLVVVNDPKKMK
                     RIFPLFIVSENINIHAEPDIEYFRVIDRAFDEVANYTGRIFSYLRTHGPLKLTSCVNK
                     QTLLSMGGYLNEWNVFDSLSRVRDFFRLSSAVFTKLDNNIYSLEVDSFCLYRDYEIAR
                     NRLMMRASHLYSEVHEFSNKHFHLNSWVKDHMPSYLNSDGVFSSFHLSELENMSPDDL
                     HEEYGNISLFNWVHAYQCLVELSKEEMSKRFSSTKPIPLQLDRWLIIKSRESWLSFFQ
                     RKGIAADAAKKLIDYFTFNSKSHDLNDCPFIPCMDGLCLMPALIANSSVTRSLMSLFG
                     SKKISQASKGRFHEQQFIKRVRDAGIKASPIDAHANYQCDCVILLDDCLIFTELKSNG
                     QPIYYGKYYQQVCNIVGDSSLIHDHNNKFMRSYFQQINRISEHYLNHLDVIIKEFELP
                     STWQPKGVYKLIVTTTMLGGKYHVDDTYVADKYALSSFFQRIPGVIYQTNENGKMAKN
                     IIDGFECCEGEITIDKFIDYLSSLPSINAVRKNIKKLIYSVRFNEKLVHHPYYDSWAF
                     GPYIRKGND"
BASE COUNT          587 a          315 c          260 g          574 t
ORIGIN      
        1 aaattgctct aatcatttcc cttacgaata tatggaccaa aagcccaaga atcataataa
       61 gggtgatgga ctaatttttc attaaatcga acactataaa ttaatttttt tatattttta
      121 cgtacagcat taatactggg caaagaggaa agataatcta taaacttatc gatagtgatt
      181 tccccttcgc aacactcaaa cccatcaatg atatttttcg ccatttttcc attttcattt
      241 gtttgatata tcactccagg tattcgttga aaaaaactag ataatgcata tttatccgct
      301 acatatgtat catccacatg atatttccca ccgagcatag ttgtagtaac aataagctta
      361 tacacacctt tgggttgcca tgttgatggt agttcgaatt ctttaatgat cacatcaaga
      421 tgattaagat aatgttcaga aattcgattt atttgttgaa aataagaacg cataaattta
      481 ttattatgat catgtattaa tgagctatct ccaacgatgt tacacacttg ctgatagtac
      541 ttaccataat atattggttg accatttgat tttagttctg taaaaatcaa acaatcatcc
      601 aggagaatca cgcaatcaca ctgataatta gcatgagcat caattggaga agctttaatc
      661 cccgcatcac gaactcgttt tataaattgt tgctcatgaa atcgcccttt acttgcttga
      721 gaaatttttt tggaaccaaa taatgacatt agagagcgag tcactgagct atttgctatt
      781 aatgcaggca ttaagcataa accgtccata cagggaataa atgggcaatc attaagatca
      841 tgtgattttg aattgaaggt aaaataatct atcagttttt ttgctgcatc tgctgcaatc
      901 cccttacgtt gaaagaatga taaccaactc tcacggcttt ttatgatcag ccagcgatcc
      961 aactgtaggg gtataggttt tgtcgatgaa aatcttttgc tcatttcttc tttagataac
     1021 tcgactaaac attgataagc gtgcacccaa ttaaataatg atatattccc atattcttca
     1081 tgaaggtcat caggagacat attttccaac tcgcttagat gaaatgagct aaaaacgcca
     1141 tcagaattta agtaagatgg catatgatcc tttacccaac tatttagatg aaaatgtttg
     1201 ttactaaatt catgaacttc gctatataga tgtgacgctc tcatcattag tctatttctt
     1261 gcaatctcat aatctcggta taaacaaaaa ctatcaacct caagagagta aatgttatta
     1321 tccaattttg taaatactgc actacttagt ctgaaaaagt ctcttactct gcttaatgag
     1381 tcaaaaacat tccactcatt aaggtaccct cccattgaaa gcaaggtttg tttattcaca
     1441 caagaagtta gtttcaatgg cccatgagtc cgtaaataag aaaatattct tccggtgtaa
     1501 ttggctacct catcaaatgc cctatcgata actcgaaaat attcgatatc aggttcagca
     1561 tgaatattaa tattttcaga aacgataaat aatggaaaaa tcctcttcat ttttttagga
     1621 tcattgacca caaccaagga tgataatgat gataaaaata tctcacaaaa catgatgaga
     1681 ttaagtatta atgcatgtga tttttttgta tttttacgcc aaataccagc acattt
//
LOCUS       DAZKHK010000336         1730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKHK010000336 DAZKHK010000000
VERSION     DAZKHK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.338"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..147
                     /locus_tag="ACHOS5_005368"
     CDS             <1..147
                     /locus_tag="ACHOS5_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9780001.1"
                     /translation="TYRKDEVIPLQRKIMNAVNSDPDIPEHLYLRFDVEAGHVAEEKT
                     VPVG"
     gene            185..448
                     /locus_tag="ACHOS5_005369"
     CDS             185..448
                     /locus_tag="ACHOS5_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9780002.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(514..840)
                     /locus_tag="ACHOS5_005370"
     CDS             complement(514..840)
                     /locus_tag="ACHOS5_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9780003.1"
                     /translation="MKTPGMTGKEIILRYLETHARFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(837..1046)
                     /locus_tag="ACHOS5_005371"
     CDS             complement(837..1046)
                     /locus_tag="ACHOS5_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780004.1"
                     /translation="MNITRNSKLKERTMNSTRRISDRELTKMIESAQQLLSRQNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
     gene            complement(1056..1460)
                     /locus_tag="ACHOS5_005372"
     CDS             complement(1056..1460)
                     /locus_tag="ACHOS5_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780005.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATRPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLALINRKAAIARYVHSAAGRQSRR"
     gene            complement(1457..>1730)
                     /locus_tag="ACHOS5_005373"
     CDS             complement(1457..>1730)
                     /locus_tag="ACHOS5_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9780006.1"
                     /translation="PPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALG
                     VQLHESAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
BASE COUNT          287 a          473 c          506 g          464 t
ORIGIN      
        1 acgtaccgca aggatgaggt gataccgctt cagcgtaaaa tcatgaacgc cgtgaacagc
       61 gatcccgata taccggaaca cctttatctt cggtttgatg tggaagccgg gcatgtggcg
      121 gaagaaaaaa cggtgccggt cgggtaaaat gtcttccccg ggatacgtgt tatcgggagg
      181 taagatgcgg atttttaaag cccggtgtcc tgactgtggc gcacctgctg tcatccggaa
      241 gacagactgg aaaacccctg aacttgcgga tctgtactgt gcatgcagtg atgtcgaatg
      301 cgggcatacc ttcgtctgta cgatgacctt ttcccatacc ctcagccccg gcgcccgctc
      361 cggtcgcaat atggtgaagt ttcttgttga tgccatgcgc ccggaagatc gccggtatgc
      421 gcttgaactt ctgtcgcggc gttcctgacg cgcttcgctt gcctgctgct tcgccggggc
      481 aaaaagaatt tttaccccga ctcaccagct cctttaacgg aatgcagcgc cgtagataaa
      541 cagaattcgc ttcatgattt cgctgcgctt gcaggcttcg atgactgatt tttcctgctg
      601 ccgttcagcc cgttgcgcca gttcctgtcg ttccttttcc gttggcttcc ggtaatgcgt
      661 tatcttgtcc gtgtacccgt ccacaaccag caacccttcc cgtaccattt ttccggcggc
      721 ctgattaatg acggttcttt tcacgcccag tttttctacc agttctgggg cgctgaaacg
      781 tgcgtgcgtt tccaggtagc gtaaaatgat ttcctttccg gtcattcctg gggtcttcat
      841 ttggcggctc cttcgttgtt ccggcgttcc cggagttcgg tcagtgctgc cagtaaattc
      901 tcccaccatg tttcttccac gaccgggttt tgtcgtgaaa gcagctgctg cgccgactca
      961 atcattttcg taagctccct gtcgctgatc ctcctggtgc tgttcatggt gcgctccttc
     1021 agtttgctgt ttctggttat gttcatggtc tttgtttacc tgcgtgattg tctgccagcg
     1081 gctgaatgca catagcgggc aattgccgct ttgcggttaa tcaatgccag tgttctggtg
     1141 agttctgcgt tgtgtttgtg tgcctgctct tcgtggtact gccgcatgat ttttccggca
     1201 ggcgtgagcg gctccggctc cccgggcgga cggcgcggac gaacggcgac aagccagctg
     1261 cggttagtgc tgtccggtcg tccgttgagt atgtcggtga tgatggccgt ggtggtgcga
     1321 tcgggactga cgaccgtgat ttccagctca tcgcctggtt gcggtctcgt ggcaatgttg
     1381 ttcagggtca gtaagggcgt gcgctggcgc atcatttcgt caaaacgctg gcgctgcatg
     1441 gcgcaataaa agcgggtcat tgtggcgcct ccgtattcgg tgcgggggtg atatcaatgc
     1501 ggcgggtgct gtcattccag tacgcggtgc gtccgtcagc caggcgcacg cgggcaccgt
     1561 gacggacgga aacgacggcg ctttcatgca gctgcacgcc cagcgcatca agctcacggg
     1621 tgatttgtgc ggtgatgtgt ggctcagcgg tcagtgcgac actcacgcga acgccgccag
     1681 cgggcgcact gatgccgccg tttttcacgg tttccgtgtc aggtggcggg
//
LOCUS       DAZKHK010000337         1726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKHK010000337 DAZKHK010000000
VERSION     DAZKHK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.339"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1318
                     /locus_tag="ACHOS5_005374"
     CDS             <1..1318
                     /locus_tag="ACHOS5_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9780007.1"
                     /translation="RIRQGEMGYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPK
                     TRSAGEREEEQAREALLALEAELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAAT
                     KRQLSGQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQQAVRFEEQQSAKQAA
                     ISAKARGLTDRQAQRESEEQRLRDVYGDNPQALAQVTGALKQTWADEDMLRGDWLAGL
                     KSGWGEWAESATDSFSQVKSAATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEI
                     LLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAG
                     IVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRVEGINFNQNNHV
                     VIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
     gene            1315..1644
                     /locus_tag="ACHOS5_005375"
     CDS             1315..1644
                     /locus_tag="ACHOS5_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780008.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRTAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            1644..>1726
                     /locus_tag="ACHOS5_005376"
     CDS             1644..>1726
                     /locus_tag="ACHOS5_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9780009.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDL"
BASE COUNT          399 a          387 c          624 g          316 t
ORIGIN      
        1 gcgtatacgc cagggggaaa tggggtacat caaccgttcg cgtgcaacag gcgtcagcaa
       61 aggtccgggg cagcaggaag ccgtcagccg tctggctgaa gagctgacag gtaaaaagca
      121 tacatcaccg aaaacgcgct ctgccgggga gagggaagag gagcaggcaa gagaggctct
      181 gcttgccctt gaagctgagc tcaggacgct ggaaaaacac agcggtgcga atgagaaaat
      241 cagccggcaa cgccgtgatt tatggaaggc ggaaagtcag tatgcggtcc tgaaagaggc
      301 tgccacgaaa cgacagttat ccgggcagga aaaatccctg ctggcgcata aagacgagac
      361 gctggagtac aaacgccagc tggctgagct gggcgacaag gttgaatacc agaaacgcct
      421 gaatgagctg gcacagcagg cggtgcggtt tgaagagcag cagagcgcga agcaggccgc
      481 catcagcgca aaagcccgtg gtctcaccga ccgtcaggca cagcgggagt ctgaagagca
      541 gcgtcttcgt gacgtgtacg gcgataatcc gcaggcgctg gcacaggtca ccggggcact
      601 gaaacagaca tgggcggatg aagacatgct gcgcggtgac tggctggccg ggctgaagtc
      661 cggctggggg gagtgggcgg aaagtgcgac ggacagtttt tcgcaggtta aaagtgcggc
      721 cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg gtgcagaggc
      781 agactggcgg ggattcaccc gttcggtgct gtccatgatg acagaaatcc tgcttaaaca
      841 ggccatggtg ggaattgtcg ggcgtatcgg cagcgccatt ggtggtgctt tcggtggtgg
      901 tgcatctgct tcctcgggga cggccattga ggctgcggcg gcgaacttcc atttcgcgac
      961 cggaggattt acggggacgg gcggcaaata tgagcctgcg gggatagttc accgcgggga
     1021 gtttgttttc acgaaagagg caaccagccg gataggtgtg gggaatcttt accgtctgat
     1081 gcgcggctat gcggaaggtg gttatgtggg tggggccgga agtccggcgc agatgcggcg
     1141 ggtggaaggt attaatttta atcagaacaa tcacgtggtg attcagaacg acggtataaa
     1201 cggacaggcg gggccgcagc tgatgaaggc ggtgtatgac atggcccgca agggggcgca
     1261 ggatgaactc cggctgcagt tgcgtgatgg cggtatgtta tcagggagcg ggcgatgaaa
     1321 acctttcgct ggaaagtgaa gccggatatg gaggtgaact cgcagccgtc ggtgcgtgaa
     1381 gtgcgttttg gtgacgggta ttcgcagcga acggcggcag ggctgaatgc tgacctgaag
     1441 acgtacaggg tgatgctttc cgtgacccgg gaggaggccc ggcatctgga agcgtttctg
     1501 gcagagcacg ggggctggaa ggcatttttg tggaagccac cctatgcata ccggcagata
     1561 aaggtgacct gtgccgggtg gtctgcgcgg gtcgggatgt tgcgtgttga gttcagcgcg
     1621 gagtttaagc aggtggtgaa ctgatgcagg atattcacga agcaagcctg aacgagtcgg
     1681 ttaagtcaga gcagtcaccg cgggtggtgc tctgggaaat cgacct
//
LOCUS       DAZKHK010000338         1693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKHK010000338 DAZKHK010000000
VERSION     DAZKHK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.340"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..315
                     /locus_tag="ACHOS5_005377"
     CDS             <1..315
                     /locus_tag="ACHOS5_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780010.1"
                     /translation="SGDDGKITISENGKVTPPSHQHCEMLIEFAIDYLKNNKKQGLMK
                     RIGRCMGYLQVAAEIEALASGADKDAVVRETVLRDFNTPPFKTEPDNWIQPGLTYLKV
                     RI"
     gene            352..885
                     /locus_tag="ACHOS5_005378"
     CDS             352..885
                     /locus_tag="ACHOS5_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9780011.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1042..1224
                     /locus_tag="ACHOS5_005379"
     CDS             1042..1224
                     /locus_tag="ACHOS5_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780012.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1239..1370
                     /locus_tag="ACHOS5_005380"
     CDS             1239..1370
                     /locus_tag="ACHOS5_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780013.1"
                     /translation="MSDKLTMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLG"
     gene            1382..1633
                     /locus_tag="ACHOS5_005381"
                     /pseudo
     CDS             1382..1633
                     /locus_tag="ACHOS5_005381"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1593..>1693
                     /locus_tag="ACHOS5_005382"
     CDS             1593..>1693
                     /locus_tag="ACHOS5_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780014.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVQCVKPP"
BASE COUNT          436 a          371 c          484 g          402 t
ORIGIN      
        1 tccggtgatg acgggaaaat caccatatca gaaaatggaa aagtaacacc gccatcgcac
       61 cagcactgtg agatgctcat tgaatttgcc attgattacc tgaagaacaa taaaaaacag
      121 gggctgatga agcgtattgg tcgttgcatg ggatatcttc aggtagctgc tgagattgaa
      181 gcgctggcca gtggtgctga caaggatgca gttgtgcggg agaccgttct tcgtgatttc
      241 aatactccac cctttaagac tgagcctgat aactggatcc agccggggct gacttatctt
      301 aaagtgcgta tttagtgggc cagggacagc ggctgaatat ttaatatatc catgaacgca
      361 aaaatcaaat acggcctgtc agctgctgtt ctggcgctga ttgccgcagg ggcttctgcg
      421 cctgaaatcc tcgaccagtt tctggatgaa aaagaaggta accacaccac ggcataccgt
      481 gatggtgcgg gtatctggac catctgccgt ggagccaccc gggtggatgg taagcctgtg
      541 attcctggca tgaagctgtc gaaggggaaa tgcgaccggg ttaacgccat tgagcgtgat
      601 aaggcgctgg catgggtgga gaaaaacatc aaagtgccac tgactgaacc ccagaaagcg
      661 ggtattgcgt cattctgccc gtataacatt ggccccggta agtgtttccc gtcgacgttt
      721 tataaacgaa ttaatgcagg cgatcgcagg ggagcgtgtg aagcgattcg ctggtggatt
      781 aaggacggtg gcagagactg ccgtatccgc tcaaataatt gctacggtca ggtatcccgt
      841 cgcgaccagg agagtgcgct ggcgtgctgg ggaatcgaca gataagcaga atattttgct
      901 gaaaaatgac gttggccaac gcgggtggat aacacgaaat cctgcgaact ggcaaaacct
      961 aagtgaataa aagtaaaaac cccgtttgtt ggcagcaagc ggggttttgt gtttctgacc
     1021 ttggataagg caagggagaa catggaaaag tataaacgaa ttctgttgag gttgactatg
     1081 aaaaacggcc ttgaactgaa agcgccagta actgatgaca tcagcagagc actggctttt
     1141 gctattaagt gggtggcggt cggtatcgct gtgtctccga tgctgtatgg gctggcaaaa
     1201 ctggtcattg cgttgaaatc gtgaagggag gattaaacat gtcagacaaa ctcacaatgc
     1261 tggcgaagat cctctgtgta atcgtcggca tttcattttc actaatgctg gttgctcttt
     1321 ttctttccat gggctggatg atgttatctt cgtcggggct gctggggtga gcataaaccg
     1381 aatgctttcc gcgtttaccg ttattctgct ggtggtctgt ggtgtgctta gtctggggct
     1441 gaatcattac cgcgataacg ccatcaccta caaagcgcag cgcgataaaa aagccagaga
     1501 gctggagctg gcaaacgcaa ccattactga tatgcaggtg cgccagcgcg atgttgctgc
     1561 gctcgatgca aaatactcga gggaattagc cgatgcgaga gctgaaaatg aaactctgcg
     1621 tgctgatgtt gccgctggtc gtaagcgcct gcggatcaac gccacctgcc ccggtacagt
     1681 gcgtgaagcc acc
//
LOCUS       DAZKHK010000339         1683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKHK010000339 DAZKHK010000000
VERSION     DAZKHK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.341"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..740)
                     /locus_tag="ACHOS5_005383"
     CDS             complement(<1..740)
                     /locus_tag="ACHOS5_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HFP9780015.1"
                     /translation="MKRRYLLSFFFVPGILMAHPFKQGGMVMDVRKSDVSEGTDIIMY
                     SPHGGDNQNFIYENGNIKLASNQNYCVDVSRNPNYKENSIILWTCNGGDNQKFTITDG
                     TIRPRDRANECITVKSEGFLKSEQCVSSPQQRFDIPKVCTYKDAYYRNMTECADSDIP
                     MVKDNDTLSSLSVVNSSGSMFEHRDFKGGKVRFDKNIPFIDDVKKGFNDKVSSLKISS
                     EKTFLITSDPQLVCEKNCNNISADTSKR"
     gene            complement(1246..1443)
                     /locus_tag="ACHOS5_005384"
     CDS             complement(1246..1443)
                     /locus_tag="ACHOS5_005384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780016.1"
                     /translation="MRQDAVIDILLIIVFSLSLLICGKVSADTGWQSGGGFQGAVEFG
                     STITLYDDRLSTTVGIPGWLL"
BASE COUNT          555 a          390 c          252 g          486 t
ORIGIN      
        1 tttctttttg atgtgtcggc agaaatatta ttacagtttt tctcacaaac caactgcgga
       61 tcggaagtaa ttaaaaatgt tttttcagat gaaattttaa gacttgatac tttatcatta
      121 aacccttttt tcacatcatc gataaaagga atatttttat caaatcttac tttaccccct
      181 ttaaaatcac gatgttcaaa catcgagcca gaagaattaa caacgcttaa agaagataac
      241 gtatcattat cctttaccat aggaatatca ctgtcagcac actccgtcat attcctgtaa
      301 taagcatctt tatatgtaca cactttcgga atatcgaatc tttgctgcgg agaagatacg
      361 cattgttcag atttcaaaaa tccttctgat tttacggtaa tacactcatt cgctctgtcc
      421 cttggcctta ttgttccatc tgtaatggta aatttctggt tgtctccgcc attacaggtc
      481 cacaggatta tgctgttctc cttataattc gggtttctgc ttacatccac acaatagttc
      541 tgatttgatg ccagcttaat atttccattt tcataaatga aattctgatt atctccacca
      601 tgtggtgagt acatgataat atcagtacct tcagatacat cgcttttcct gacatccatt
      661 accatacccc cttgcttaaa gggatgggcc atcaatatgc caggaacaaa aaaaaagctt
      721 aaaagatagc gtcttttcat aaaaacccca tagagcaaat gtataaaccg aatataaata
      781 aaccttcaaa aaataaattt tacacacctc ctcatttaaa acaaaaatgc tcagcatagc
      841 tataaaaacc aatatcaaaa agattcacca gatgtgagtt caatatatct acaataatgg
      901 ataagcaaca gcagaacagt gaaatacaaa gagcagtaga tacgggaaac tgctgtctga
      961 attatgtaga caaaatccgc aattatttta tctgatgact tcctgttttc agtactaaaa
     1021 atacgctacc gcccataccg tgatcagcgt acggccatag cgaaataata aagaaagcag
     1081 cgatttcatt attctgatgg catgccaccg gcatcatgta ttatggtgct tcattgacta
     1141 tacatccgga aattccgccc acagcctgca atcaaagcag gtcataagaa accgcagaag
     1201 gagacagctt cctgactctc tcagcaaaaa taccagtgat aacggttata ataaccaccc
     1261 tggtattccc actgtcgtgg aaagtctgtc atcataaagc gtgatagtgc ttccaaactc
     1321 cacggctccc tgaaagcccc caccggactg ccatccggta tcagcagaaa cttttccgca
     1381 aattaataaa gaaagagaaa agacaataat aagtaaaata tcaattacag catcctgacg
     1441 cacatccact cccccctgag acaccttcat ctgtattatt cactcgtggt ggaatttctt
     1501 ttatctttct gtaaacctca ctgaagtcat cttccaccac cacatgacct tcaattcccc
     1561 tgatatgaat ttcacaaaaa acacccttct gaagaacgaa actgatgtct tttatattca
     1621 ccagaagatt tcgcccctgc ctgctgacaa agtgaacaaa cacgcctgcc tctcaatgca
     1681 aat
//
LOCUS       DAZKHK010000340         1622 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKHK010000340 DAZKHK010000000
VERSION     DAZKHK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.342"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..389)
                     /locus_tag="ACHOS5_005385"
     CDS             complement(<1..389)
                     /locus_tag="ACHOS5_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9780017.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPADGEGTVISTTSEPEYYFVIALAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYND"
     gene            complement(1404..>1622)
                     /locus_tag="ACHOS5_005386"
     CDS             complement(1404..>1622)
                     /locus_tag="ACHOS5_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFP9780018.1"
                     /translation="NRPYIAEEKVVIDIVQVTAELLVRQTRRRKLEDEHNAFVWSLMS
                     DDQRTEVSRFLSVHGLFFDAGSGCVHIR"
BASE COUNT          401 a          458 c          392 g          371 t
ORIGIN      
        1 atgtcgttat acttacaggc ggcgcctccc ggtgtcaccg tactgcgacg cgccagctgc
       61 ttaatacgcg ggtccggacg gtcatatgtc tccggcagag gaaggccttc accatatgac
      121 atgccgtttg actgccccgc cagggcaata acaaagtaat actccggttc gctggtggtg
      181 ctgataactg tgccttctcc atccgccggc ttcaccacca caggtgtggt gacatcacct
      241 tccgccgcaa tggcctgcat cagggtataa ggcgtgatgg ccaccggact gccaaatggc
      301 tgccacccct ccttcagttt ttgagtcagt cgctccgcaa ggtctgacgg cgacgccgcc
      361 ctgaccacat cgtagtgttt aaatgccatg aatcctcccg gtcgggataa tgttgtgagt
      421 cagataaggg gcgggctgaa gtccggaagt tacaggacaa tgacaggagg aagactacag
      481 cccgcaatac gaaaaaggcc gcgcagttgc gcagccttat gaattctggt taaaatctgt
      541 ccgctaaatt cattgctttt tgaaagtgtt aatcatgttt gcccgcacat tgttgcgggc
      601 ttttttatcc ataagaaagc ccctccggag aggggctgga gtagcgctat gtaccgttca
      661 ttgcatgatg ccgggtgcct cccggtgagt tcagcccggt gccaccgaac ccgcgtcatt
      721 ctcgctaacg atcatcagag accataccgt acaccagccg cccctccgca cagggggatt
      781 caccatgcag aaatttttta acaaattctc agtcgggaag acaaccttca actgactgaa
      841 ttgtgagcaa catagcattt aacggggaac ctgttttctg cagtaaaaag gcccaccgga
      901 gcggacgggc ctggaaggat agcggtcatg tgatgccggt ttcccggtaa ctcagcaccg
      961 gtatctgagt caacgttttc tctactgggt catttccgat acgccctgcc tgctgacagg
     1021 ctttcatcac atctgaaaat atagcaccct gactgatact gtagtaccca aggttccaga
     1081 aactgtgatg tatccggcac agaaaagccc ctccggagag gggctgacgc tgcgtatctg
     1141 tatcatcatg cgcatggtgc cgggtgcctc ccggtgagtt cagtatcagc acctgaaccc
     1201 gcacagaaag gataagggat gtaaccaaac acctgtcgct gatatgcccc tccgctcagg
     1261 gggattcacc atgccagttt cttttaacaa actccccgca aatcagacaa ctgtcaaccg
     1321 tctgaattgt gagacattta aaattttcgg ggcaacagga agacaatcat cacgacaccg
     1381 gcaaccagcg accggcacca gatttaacgt atgtgcacac aacctgaacc ggcatcaaaa
     1441 aacaaaccgt gcactgacag aaaacgggaa acctccgtcc gctgatcatc actcattaag
     1501 gaccacacaa aggcgttatg ctcatcttca agtttccgtc ttcttgtttg ccttacaagc
     1561 aactcagccg tcacctgtac aatgtcaatg accaccttct cttcagcaat gtacggcctg
     1621 tt
//
LOCUS       DAZKHK010000341         1608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKHK010000341 DAZKHK010000000
VERSION     DAZKHK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.343"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            110..1129
                     /locus_tag="ACHOS5_005387"
     CDS             110..1129
                     /locus_tag="ACHOS5_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9780019.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGGENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPVTN
                     KNGEDVNTGWHAIVKGRSAKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEEA
                     EDVGV"
     gene            1147..>1608
                     /gene="gpM"
                     /locus_tag="ACHOS5_005388"
     CDS             1147..>1608
                     /gene="gpM"
                     /locus_tag="ACHOS5_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP9780020.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRN"
BASE COUNT          369 a          497 c          466 g          276 t
ORIGIN      
        1 aacttaacga cctgaccacg aagctgtcag caaccggcat cacgccacga ccggctggca
       61 caccggcaga aagcgaaaaa ccgtttgact ttgtttaagg acacgcaaca tgcaactgaa
      121 ccagaaaacc gaatcactgc ttcgtaagta cgctgccgga ctgggcgcgg caaacggggt
      181 tgacgacgtc acacgctatt ttgcgctgtc tgacccgaag gagaccgcgc tgcgtgacgc
      241 catcatgcac tccgacgagt ttttatcaaa actgccgaac gtgctggatg ttgaacaggt
      301 gacaggccag gtggttacca ccggcgttcc gggcctccat accggacgta aaaaaaatgc
      361 ccgtttcacc cttccgctgg gtgttaccgg gaatgaatac aagctggctg aaaccgattc
      421 cggtgcccac ctcctgtaca ccacactggt cacctgggca aacgcaggcg gtgaaaacga
      481 gttctttaac cgcattcaga attttgttaa cagacagatc gcgctggata tcctgcgcat
      541 cgcctttaac ggtaccagcg tggccgaaga taccgacccg gtaaccaaca aaaacggcga
      601 ggacgtcaac accggctggc atgccatcgt caaaggccgc agcgcaaaac agatcgtgga
      661 ggctggcccg acgctgggta aagggggcga ctttatcggg ctggatgcag ccgtggcaga
      721 cctcgtgcac accctcattg atgagtcgta tcgcggtcat ccggatctgg tggtactggt
      781 tgcgcccgac cttgtggcac aggacaaggt gacgctgctt aacgccatcg acgcccccac
      841 ggaaaaagtg gccgcgcagc ttatcggtcg ccagatcgcc gggctgacag catacacccc
      901 gccgttcatg ccggaagggc gcctgattgt caccacgctg gcaaaccttc acatctacac
      961 gcagcgcggc acccgtcagc gccgtgcgga gtggaacgac gatcgcaagc gttttgaaaa
     1021 taattacctg cgcatggaag gttacgccgt ggagcacgac gaaatttacg cggcctacga
     1081 caagctgacc atcaagggcg cagaagaagc cgaagacgtc ggcgtataag cacagagggc
     1141 ggcgcaatga caccctgcca gcgacaccgg gcgaaaatcc gcacacagga ggcactcgaa
     1201 cgccgggagg cgctgacggc ctccccggtc agcttccacc tgttacgcgc ggagcttgat
     1261 cgcgatgtgg cacggctgcg cagtcttccg gtccgcgaag agcggctggc gttcaagcgc
     1321 gacattctcc tgccgcgctg gctgcccgtc gcggagcgct atatcgctga cggaaagcgc
     1381 cacgcctgcc cggtactggt gtactgcatc atctggctgt tcgacacggg cgacctgagt
     1441 cgcgcccttg actgggcaga cattgccatc agtgagggcc aggccacgcc ggacaacttc
     1501 cgcagcaccc tgcccgcctt cgtggccgac acggtgctgc agtgggccac cgacaacagc
     1561 gaggccggac acagcataga gccgtatttt tcgcgcacct tcaggaac
//
LOCUS       DAZKHK010000342         1583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKHK010000342 DAZKHK010000000
VERSION     DAZKHK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.344"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            416..>1583
                     /locus_tag="ACHOS5_005389"
     CDS             416..>1583
                     /locus_tag="ACHOS5_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP9780021.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQGGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTV"
BASE COUNT          368 a          299 c          511 g          405 t
ORIGIN      
        1 ggtcttaagt acaggagtgt gaagcacctg gttggacgtg cgtagccgga atgcatattc
       61 gtgagtcggg taatgagagt ggaacggtgg cagtgagaat ggaacagaaa aaaatcagat
      121 gcggaaagcg gaaatacaca gcgggtgcga tgtgcttcat gctgtttgta tggctggggg
      181 gtgtggtgac attctgtgtg ctggccgctc tgataatgag gtgaaaacgg aagagagttt
      241 acttccttaa ttcgaacttt ctgataactg agaacttgtt aaatgcctgt gggtttatgt
      301 atttaaatca attggttaat ggtgatttta tctttatcag tcaaagttcg ttttagtatg
      361 atgattgtgc ccgctgttta ttgttgtccg ggagaaaata cagggaatcc aggggatgaa
      421 caaaatatac tcactcagag ttaatgacag aaaagaactg gttgctgtac cggaaacggt
      481 ggggggcagg aaaaagtcgt ccggaattcc cggtgcgggg atgctttccc gtcttcttct
      541 ggcctcagga gccgttgccg gcgttctgtt ttcttctcct gcgctggcat ccgttgtcgg
      601 gaatactctg ccatggcaga cctacaggga ttttgctgaa aacaaagggg cgtttcatgc
      661 cggggccaca aatattccgc tttatggtaa gaatggggct gtcagtgggc ggctggacaa
      721 ggctccgatg atggatttca gtgttgtgga ccagatactg ggggtggcaa cactgatttc
      781 tccgcagtat gtggcggggg ttaagcataa cggcagctac aatacggtgc gttttggtta
      841 tgcggacgat acgacatatc gccttgttga ccgtaatgag cactggcgtg attttcatac
      901 tccccggctg aacaaactgg tgacagaagt tgccccggtg agcgtcacgg atgccggaac
      961 cggcaagggg gtataccaga accgcagtcg ttatccggtg ttttatcgta tggggagtgg
     1021 aactcagtac accggagctg cttccggtgc gctgaccaga atagcggatg cttacgtctg
     1081 gaggactggc gggacggtgg gaagtccggt catttctgac tggagtctgg tgtcaaaccc
     1141 gggaaatctt taccagggtg ttaatggtcc gctggcgtct tacgggacat caggagacag
     1201 tggctcaccg ctttttgcct gggatgcggt gaagaagcag tgggtatttg ttgctgtact
     1261 tcatggttat gccggtgaga acgggaagac gaactggttt acggtgattc ctgcagggga
     1321 tgtgaataac acgataaagc aggacaattc aggcacagtt gtgccggcag ttgccggtgg
     1381 tgacattgtg tggaagtaca gtcagggaag cggagaggga accctttctc agggcgggca
     1441 ggtatggaag atgaacggct tcaggggagg gagtctgaat gacgggaaag atatcacctt
     1501 tgggggtaag ggcacggttg ttctgaagga tgatgttgtc cagggggcgg gttcgctcac
     1561 gtttaatggt gattatacgg tca
//
LOCUS       DAZKHK010000343         1575 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKHK010000343 DAZKHK010000000
VERSION     DAZKHK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.345"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(93..1484)
                     /gene="narK"
                     /locus_tag="ACHOS5_005390"
     CDS             complement(93..1484)
                     /gene="narK"
                     /locus_tag="ACHOS5_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP9780022.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          475 a          428 c          380 g          292 t
ORIGIN      
        1 aataagccat ataatatgag tatgttgtaa ctaaatgacg aataaaaaaa gcgcgacatc
       61 atgccgcgca aaggataatc aaatattaag atttattttt tagaatgccg accatatacc
      121 gcccaggtaa tcaccacgca ggcgatatag aaaatcaaaa atactttcat tgcgccgact
      181 ggcgaacccg ttaatgccag cgagctacca aacgctttcg ggataaagaa gccaccaatc
      241 gcgccaatcg cagagatgaa acccagcgct gccgccgtgt cggttgccgc ttcacgcatc
      301 gcacgttcgt cagaaccccc ttctgctttc acgcgatcca ttgtcagttt acggaagatc
      361 acggaaatca tctggaaagt ggaaccacta cccagcccag ctgtcaggaa cagcgccagg
      421 aagactgcga agaacgccat gaagcttccg ccctgtccgt cagtcggtaa ggtcaggaac
      481 agcaggccgc tgaaaatcgc catcaggata aagttcacca gcgtgacacg agttccgccc
      541 agacggtcag ataatgcacc acctgcagaa cgcgccagcg caccaataaa cggcccgaag
      601 aaagcgtatt gcagaatctg aacatccggg aactgcgttt ttgacagcat cgcaaagccc
      661 gcggagaagc cgatgaagga gccgaaggtt gccagataca gcaggctcat aatccacaga
      721 tgaccccgtt tgagtaccgg caactgctcc ttgatggagg ctttcgaggt agcaagatcg
      781 ttcatgccaa accacgccgc aatggtgaag atggcaagga acggcaccca tatccaggac
      841 gcattcgcca gatacagctc agtcccatcc ggctgtttga cgccctggct accaaatact
      901 gcgaaaatcg acagtgatac caccagcgga gcaaccaact gcatgacgct gacgcccatg
      961 ttgcccagac caccattcag acccagcgcg ccaccctgct tctgtttcgg aaagaagaag
     1021 ctgatgtttg ccatactgga tgcgaagttc gcgccagcaa agccgcacag cagagaaatg
     1081 atgatgaaga cgctataagg cgtggaggta tcctgcacgg caaaacccag ccagacgcaa
     1141 ggaataatca gaataccggt gctgaacgcc gtccagcgac gaccaccaaa gataggaacc
     1201 ataaaggagt atggaacacg taataacgcg ccagaaaccg aaggcagcgc agtcaacata
     1261 aatagctgat cggtcgtaaa attaaaaccg actttcggta agttcaccgc aacagcgctg
     1321 aacaacatcc atacgcaaaa cgccagcagc agacagggaa cggaaatcca caggttgcgg
     1381 ctggcaatac gttgaccgcg ttgttgccag aacgcaggat cttccggtcg ccaatctgta
     1441 atgacagctc cagtagccct ttcgggggcg gatgagtgac tcatagacac ctctgatact
     1501 cgtttcgatg tctgccacct tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg
     1561 ataagataac tttat
//
LOCUS       DAZKHK010000344         1564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKHK010000344 DAZKHK010000000
VERSION     DAZKHK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.346"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..636
                     /locus_tag="ACHOS5_005391"
     CDS             <1..636
                     /locus_tag="ACHOS5_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780023.1"
                     /translation="SQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGDAVASLIRK
                     NNYLQSRVQQSTQQAEGNGGEYLSPVLDAMNAVPVLKTWQESDPDRFSVAVSIDGKLQ
                     NDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKVKTAEQEQA
                     VPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKLG"
     gene            660..>1564
                     /locus_tag="ACHOS5_005392"
     CDS             660..>1564
                     /locus_tag="ACHOS5_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP9780024.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAG"
BASE COUNT          401 a          378 c          429 g          356 t
ORIGIN      
        1 tctcagatcc accaggccgg tatgaagccc acaccgttac cggaaaacga aaaaatttct
       61 gatgagcaga ttgcccgtat cagggagatg tatccggaaa ttggtgacgc ggtggcttcg
      121 ctcatccgta aaaataacta tctccagtcc cgtgttcagc aatcaacaca gcaggcagaa
      181 ggtaatggtg gtgagtattt atcaccggtt cttgatgcga tgaatgccgt gccggtgctg
      241 aaaacgtggc aggagtccga tccggatcgc ttctcggttg ctgtatccat cgacgggaag
      301 ctccagaatg accccgcatg gaaagacaaa acgctcactg aacgtttcgc tgaagtggcc
      361 cgtcgtacgc aggttgcttt cggtgaagtc agtgagtcgt ctgctgacaa ccaggcagac
      421 aaaacggata tccggaaaac ggcggaagaa aaagtgaaga ccgctgaaca ggagcaggca
      481 gtacctgctt ccccgtcaga tttaggcacc acggcctccg tcggaaccgg tgataatttt
      541 gaacggttac ttggtgcttc tcattcagag gctgaggcga ttatgcgtgg tatgacgaat
      601 gctgaaatag acgcacttct ggagaagctc gggtaactta ctgaaggagt actgaagtaa
      661 tgacgactgt aacatcagcc caggcgaata agctgtatca ggtggcgctt tttaccgctg
      721 ccaaccgcaa ccgctcgatg gttaatatcc ttactgaaca gcaggaagcg ccaaaagcgg
      781 tttcgccgga caagaaaagc acgaagcaga ccagcgcagg tgcgccggtt gtccgtatca
      841 cagaccttaa caaacaggcc ggtgatgaag tgaccttcag catcatgcac aaactctcaa
      901 aacgtccgac gatgggagat gagcgtgttg aaggtcgtgg tgaggatctc agccatgctg
      961 acttctccct gaaaatcaat cagggacgtc acctggtgga tgcaggcgga cgtatgagtc
     1021 agcagcgcac gaagtttaac ctggcatcct ctgccagaac gcttctgggg acgtacttta
     1081 atgacctgca ggaccagtgt gcgatagtgc atcttgcggg agctcgtggt gattttgttg
     1141 ctgacgacac cattctgccg acagcggagc accctgaatt caaaaaaatc atgatcaacg
     1201 atgtactgcc tccgacacat gaccgtcact tttttggcgg tgatgcgaca agctttgagc
     1261 agattgaagc ggcagatatt ttttctattg gcctggtgga caatctctcc ctgttcattg
     1321 acgaaatggc gcatccgtta cagccggttc gtctgtccgg tgatgaactt cacggagaag
     1381 atccatatta cgtcctgtac gtcacgccgc gtcagtggaa tgactggtac acctcgacgt
     1441 ccggtaagga ctggaaccag atgatggttc gtgccgtgaa ccgtgcaaaa ggttttaatc
     1501 atccgctgtt caaaggtgaa tgtgcgatgt ggcgcaatat cctggttcgt aagtatgcgg
     1561 gtat
//
LOCUS       DAZKHK010000345         1548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZKHK010000345 DAZKHK010000000
VERSION     DAZKHK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.347"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(583..873)
                     /locus_tag="ACHOS5_005393"
     CDS             complement(583..873)
                     /locus_tag="ACHOS5_005393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780025.1"
                     /translation="MIVYPALLFCRLKILCVFLLPVLQKKPSRHGSGSALSAAGNRLG
                     RTPDPHRCNTDGLRQPTNRMAACSVQMGFAGGWRGSWRPESPWLGVSARLTS"
     gene            complement(921..1397)
                     /locus_tag="ACHOS5_005394"
     CDS             complement(921..1397)
                     /locus_tag="ACHOS5_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9780026.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          355 a          411 c          385 g          397 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca cgcacgccga tacgattacc
      301 acctggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccgttgtt gttctttcgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcacaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggttgt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggca gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttagcac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccaac gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cgcgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat ttttccctct gaaaatccca tgcactaacg ttggttcaaa
     1441 tctaatccat catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc
     1501 gacaacgttg tgtcaccctg gatgcgacag gtaaataaaa aacacact
//
LOCUS       DAZKHK010000346         1546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKHK010000346 DAZKHK010000000
VERSION     DAZKHK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.348"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..234
                     /locus_tag="ACHOS5_005395"
     CDS             <1..234
                     /locus_tag="ACHOS5_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780027.1"
                     /translation="ELFNSKDKTTSNMIITHVMDHEFVCKLIDLAMRNAEKHLLKKAW
                     KKNAAEKLSEVDFKEVRQALAKMHYTVLAESIC"
     gene            308..550
                     /locus_tag="ACHOS5_005396"
     CDS             308..550
                     /locus_tag="ACHOS5_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9780028.1"
                     /translation="MIDEFHVMYMYKKIQAEAATTDIKTLEQLLKNFRSVVNERREEY
                     YQEIGEKKARKLKMKRLQRMLEKMACDRVSPEDLKE"
     gene            655..1005
                     /locus_tag="ACHOS5_005397"
     CDS             655..1005
                     /locus_tag="ACHOS5_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFP9780029.1"
                     /translation="MGFEAKKKHAIAIMESKKMWRSNYAPPLLHLAWKAGLKIPPLPF
                     ASFWQITLLMGGWFGPAWGICMWFFTWKDTGIQPGTAFVSSVVAGICFGVLMAAYHWW
                     RKVVNNLPDWDSLG"
     gene            complement(1069..1473)
                     /locus_tag="ACHOS5_005398"
     CDS             complement(1069..1473)
                     /locus_tag="ACHOS5_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP9780030.1"
                     /translation="MSEALKALNNIRTLRAQARETDLATLEEMLEKLSAVVEDRREEE
                     NSARKEQEERQAKLEAFRQKLLEDGIDPTELLASVGSSQPKTKSTRAPRPAKYKYKDE
                     NGNEQTWTGQGRTPKAIAAAIEAGKTLEDFAI"
BASE COUNT          440 a          279 c          375 g          452 t
ORIGIN      
        1 gagcttttta actcaaagga taaaacgacc agcaatatga ttataacgca tgtaatggat
       61 catgagtttg tttgtaagct aatcgatctg gcgatgagaa atgctgaaaa gcatcttctg
      121 aaaaaagcat ggaaaaagaa cgcggctgaa aaactatctg aggtggattt taaagaggtt
      181 aggcaggcat tagccaaaat gcactacact gttctggcgg aatcaatatg ctgatatatt
      241 ctgacgtcta tcggcccctt cttggggctg ataacaaatc ttatttgtct ttccaaggag
      301 caaaaaaatg attgatgaat ttcatgtgat gtatatgtat aaaaaaatcc aagcggaagc
      361 cgcaactact gacatcaaaa cacttgaaca gcttttgaaa aatttcagga gcgtggtcaa
      421 cgaacgtcgt gaagaatact atcaggagat tggtgagaaa aaagcgcgta agttgaaaat
      481 gaaaagatta caaagaatgc ttgagaaaat ggcatgtgac cgggtttcac cagaagatct
      541 aaaggaatga ctatttgagt tatttttgac ttacatacat ggaattgata atcattattg
      601 tctattgtgg ctttttatcg atagatgagt aaaggtggag ctggagggaa ccgtatgggc
      661 tttgaagcta aaaagaaaca cgctattgcc atcatggaaa gcaagaagat gtggcgaagt
      721 aattatgccc ctcctttgct acacctcgca tggaaagctg gactcaagat accaccgcta
      781 ccttttgcct cattctggca aatcactttg ttgatgggtg gatggtttgg tccggcatgg
      841 gggatctgta tgtggttttt tacttggaag gatacgggga tacaaccggg cactgcattt
      901 gtctcaagcg tcgtagcagg tatttgtttt ggtgttctta tggctgcgta tcattggtgg
      961 cgaaaggtcg ttaacaacct gccagactgg gatagtcttg gatgaataat cgaatatttt
     1021 ccgggcctgt tatcttgaga acagtgaagc ccggatatgc tgtagcggtt agattgcaaa
     1081 atcttccagt gtcttaccag cttctatagc agcggcgatt gctttaggag tacggccctg
     1141 acccgtccaa gtctgctcat taccgttttc atctttatat ttgtatttag cggggcgagg
     1201 agcgcgggtt gatttggttt taggctggga tgaaccaact gaagcgagta gttctgtagg
     1261 atcgataccg tcttctaaca atttctggcg gaaggcttcc agtttagcct gacgttcttc
     1321 ttgttcttta cgggctgaat tttcttcctc acggcgatct tcaacaacag cagagagttt
     1381 ttccagcatc tcttccagag ttgccagatc tgtttcacga gcctgtgcgc gaagtgtacg
     1441 aatattgttc agtgccttaa gagcttcact catagtatgt catccggtgt tttaaaatag
     1501 gcgattaata aaaataatgc ttacggtgct gttggaaaaa tcaaaa
//
LOCUS       DAZKHK010000347         1541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKHK010000347 DAZKHK010000000
VERSION     DAZKHK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.349"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..910
                     /locus_tag="ACHOS5_005399"
     CDS             <1..910
                     /locus_tag="ACHOS5_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9780031.1"
                     /translation="VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWA
                     RRGIISDRLATAILSCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKP
                     YGWDGMDGRRTLVDDSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFK
                     IPENVNLETTRNKYAFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQK
                     ELGTFGAFDHDWHTLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITD
                     ITGSAETYPVLIDTVEVKANKAGAAA"
     gene            1050..1232
                     /locus_tag="ACHOS5_005400"
     CDS             1050..1232
                     /locus_tag="ACHOS5_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9780032.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            1258..1530
                     /locus_tag="ACHOS5_005401"
     CDS             1258..1530
                     /locus_tag="ACHOS5_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9780033.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT          434 a          328 c          416 g          363 t
ORIGIN      
        1 ggtgaatgtg ccgacgaaca aaccggaaga agacccggat attccggata tcgggtatta
       61 cggttcaaaa tggcgaacgg acagaagcac ctggacatct caggacaggg ccagtcattt
      121 cagttcatgg gctcgccgtg ggattatttc cgaccgtctg gcaacggcga ttctgagctg
      181 cgcgggtaag tcttctgcgt ttgttaatgg tactgccggg gtggttgttc cagacagacc
      241 ggttaccacc tcagagtctg taatttttta cgatgccaaa aaagcttcag acaatcagct
      301 gaaaccttat ggctgggacg gtatggatgg cagacgcaca ctggttgatg acagcggcaa
      361 taaagctctg cgaattgaga aaaataacag cgcgaaatcc tggtcaatgt actgtgatat
      421 tgctgcagac aaggcaaaac ttttactgga aaaaggcggg gaaattgctg tccggtttaa
      481 aatccccgaa aacgtcaatc ttgagacaac cagaaacaag tatgcctttg gtttgtactg
      541 gcgaatagcg gaatggccgg gtgagggtgg tgaaggctat ctgagttctt tctttgtcca
      601 gacagataaa gccagtattg atgttgcata ccatcataca gttaatcaac aaaaagaact
      661 tggcacgttt ggcgcattcg accatgactg gcatacgctt gcatttaaat ttaagggcag
      721 taacagcatt aatgttactc cggtgcttga tggtgtggat ggacaggcgt ttgacctggt
      781 gaaatgggcc aatactgcta atggactcaa caggtttgtc attacggata ttacaggtag
      841 tgcagaaacc taccctgtac ttattgatac ggtggaagtt aaagcaaaca aagctggagc
      901 agccgcataa ttgctaaaaa aagccgccag cggcaggaat ggaagctggc ggaggtaatc
      961 ccaatggaga atgtaaagaa aagatgcttt cgtacattgg ttttttaaat gaaaacagtt
     1021 ctcattgtca accataacgg taagaaatta tgatatttat tcatcaggtg atgctgtact
     1081 tctgtacggc ggtctgtgtg ctgtatcttc tttcgggtgg gtacagggca gtgcgcgatt
     1141 tctggcgcag gcagattgat aaaagggccg ctgagaaaat cagcgccagt cagtcagccg
     1201 gaagcaaacc cgaagagccc gttactcctt aacaacccct ttcagcgaga aaatcccatg
     1261 tcagaaatta catccctggt cactgcagag gcagtgaagg aagtcctgcg ctctgaagaa
     1321 gtccggagcg cactgaaaca gaaacttcgc cagaatcttg aggcgcgtct tgatgcagaa
     1381 gttgatgcca ttctggatga gctgcttggt gtacaggcag agccaccgac tgaagcggga
     1441 gataccaccg cagagagcgg tgaagttcag cctgaatcac cggtcgccga tgcgactgaa
     1501 cctcaacccg aatcggtcat gatgctgtaa cgaggagtca g
//
LOCUS       DAZKHK010000348         1540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKHK010000348 DAZKHK010000000
VERSION     DAZKHK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.350"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..204
                     /locus_tag="ACHOS5_005402"
     CDS             <1..204
                     /locus_tag="ACHOS5_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFP9780034.1"
                     /translation="IVTSVIFSALHLQYTDFRTFLMLFLVSLVLISAKIKSNGLLMPI
                     LLHMSMNAVIAGIQYLAYISYTH"
     gene            complement(379..657)
                     /gene="mcmA"
                     /locus_tag="ACHOS5_005403"
     CDS             complement(379..657)
                     /gene="mcmA"
                     /locus_tag="ACHOS5_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin McmA"
                     /protein_id="HFP9780035.1"
                     /translation="MRKLSENEIKQISGGDGNDGQAELIAIGSLAGTFISPGFGSIVG
                     AYIGDKVHSLATTATVSPSMSPSGIGLSSQFGSGRGTSSASSSAGSGS"
     gene            complement(911..>1540)
                     /gene="mchF"
                     /locus_tag="ACHOS5_005404"
     CDS             complement(911..>1540)
                     /gene="mchF"
                     /locus_tag="ACHOS5_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFP9780036.1"
                     /translation="VSLETTDLSYRYDSQSAQVFSGLNLSVAPGESVAITGASGAGKT
                     TLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDDRLFSGSIRENICGFA
                     EETDDEWMTECARASHIHDVIMKMPMGYETLIGELGEGLSGGQKQRIFIARALYRKPG
                     ILFMDEATSSLDTESERFVNAAIKKMNITRVIIAHRETTLRTVDRIISI"
BASE COUNT          481 a          343 c          242 g          474 t
ORIGIN      
        1 attgtaacat cagtcatatt ttctgcgtta catctacaat atactgactt tcgtacattt
       61 ttgatgctct ttctggtatc gttagttctt attagtgcaa aaataaagtc gaatggcctg
      121 ttaatgccca tattacttca catgtcgatg aatgcagtca tagcaggaat acaatatctg
      181 gcttatatca gttatactca ttaacagagc attttctgtt acaatctcat atgataaaaa
      241 ttaaacggac tggcattgtt actatgcggt atgcataaaa tgataaacgt tcggaggagc
      301 ctaacttaaa ccgttacata ggcaccatta tcatataatg aagcacctgt tacaggtgct
      361 tcattaacaa tataaggttt aacttccact ccccgcagac gaagaggcac ttgatgtacc
      421 tctgccagat ccaaactggg atgataatcc tatacctgag ggagacatgg agggacttac
      481 cgttgcagtc gttgccaatg aatggacttt atcacctata taagccccta caatagaacc
      541 aaatcccgga ctaataaacg taccagcaag tgaaccaata gcaattaatt ctgcctgccc
      601 gtcattcccg tcacctccag atatttgttt tatttcattt tcagatagtt ttctcatttc
      661 aacaccttcg ctataagatt tgtgaaaaag taaaaattta cgtttaacca caacgataaa
      721 aatgtaacta caagtgatag aaaaaacata tatagattta aaaaattata cccatagtaa
      781 taactaagat ataatgacac cgggatataa aagaacagat aattgataac aaaagcaatt
      841 actgttattt ttacatcctt cttaacctcc cccatcgaca aaactcctta cacagttaca
      901 ccagtggatt ttaaatagaa ataatcctgt caacagttct caacgtagtt tctctgtgtg
      961 caataatcac ccgggtgata ttcatctttt ttatagcagc attcacgaaa cgttcgcttt
     1021 ctgtatcaag agaacttgta gcctcatcca taaataatat tcctggtttc cggtataagg
     1081 ctcgtgcaat aaatatacgc tgtttttgac caccagaaag tccctctccc agttcaccta
     1141 ttaacgtttc ataccccatt ggcattttca ttatcacatc atgaatatga cttgctctgg
     1201 cacattctgt catccattcg tcgtctgttt cttctgcaaa cccacagata ttttcacgaa
     1261 ttgagcctga aaatagccgg tcgtcctgca taacacaggc tatcatacgg tgataattat
     1321 ttattccaag ttgacgtata tccgtgccat taaccaatac ttttccacta tctggttcaa
     1381 acagtccaca taatactttc attaatgtgg tttttccggc accggaggca ccagttatag
     1441 ccacactttc tcccggagcc acagacaaat ttagaccact gaatacctgt gctgactggc
     1501 tgtcataccg gtagcttaaa tcagtggttt ccagtgaaac
//
LOCUS       DAZKHK010000349         1472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKHK010000349 DAZKHK010000000
VERSION     DAZKHK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.351"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1472
                     /locus_tag="ACHOS5_005405"
     CDS             <1..>1472
                     /locus_tag="ACHOS5_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780037.1"
                     /translation="PEHNTDNTWAYPTNAELDGGIREAFDHLFRTLKTRETDKGVAFY
                     SRKGVTRTPEGNLISDVNRSKEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQKEA
                     EALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPA
                     EYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAA
                     WERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYS
                     RSGKPDPFKADAYDAKQFASEVRRIAGLQSVPRGGEVRVGDTPPVLRALGAPNKELVM
                     PAHVVHKATRPELREHYVSVETMADLPQLLADPVAVMTSKTEHDSLVTLIEAKDRRGA
                     PVIVAIHMRAKGAVAEVNKIASVYGKDNRIALQEQLDTSLLYLNKEKAADWLLSAGLQ
                     LPGANTINGSSGFKVLHPEDIRKGAYYSRTSSLTPEETIAS"
BASE COUNT          368 a          351 c          436 g          317 t
ORIGIN      
        1 cctgagcaca acacagataa cacctgggct tatccgacga atgcggaact ggatggcgga
       61 attcgtgagg cattcgatca cctgttccgc accctgaaaa cccgagagac ggacaagggc
      121 gttgcgtttt attcccgtaa gggcgtgacc cgcacacctg aaggcaatct catttcggat
      181 gttaatcgca gtaaggaagc caaaggcagc ccggtcccgc aggttgaagc ggttgcccgt
      241 ggcgtgatga gcggcattaa ggacagtgac ctgaaggtcc gtgtggtgaa gtcacagaaa
      301 gaggctgaag cgctggcggg tgaatcgttc gacggttatg gcagggtgca cgcgttctac
      361 cgtccggata aacgtgaaat tgtcctggtg gcggataaca tccctgacgg gcggaccgtt
      421 cgcgagaagt tgcgtcacga gataattcac catgccatgg agcacgttgt cacgccagcg
      481 gaatatcaga cgattattaa gaccgtactg aaaacccgcg acagtgataa cgccaccatc
      541 cgtgaagcct ggcgtaaggt tgatgcgtcc tatggtaagg aatcaccgga agtacaggcg
      601 ggtgaatttc tggcacatat ggcggagaaa cagccgaata aattcgtggc ggcatgggag
      661 cgtgttgttg ccctggtcaa aggggtactg cgtcgtacgg ggttactgaa gccgacggaa
      721 ctgaacgata tcagacttgt tcgcgagacc atccgtacgt taggccagcg tgtgcgggaa
      781 ggttacacgc cgcgtgagga tggcgcgggc gcatcgtctc agtactcccg tagtggtaag
      841 ccggatccgt ttaaggctga cgcctatgat gcaaagcagt ttgcttcaga agtgcgtcgc
      901 attgccggat tacaatcggt tccacgaggt ggagaggtgc gggttggtga cactccgcca
      961 gtgttaagag cacttggcgc accaaataaa gagctggtta tgccagctca tgtggttcac
     1021 aaggccacca gaccagaatt acgtgagcac tatgtttctg ttgaaacgat ggctgactta
     1081 ccgcaattgc ttgccgaccc ggttgcggtg atgacatcaa agacggaaca tgactcactg
     1141 gtaactttga ttgaggctaa ggacagaaga ggtgcacctg tcattgttgc aattcacatg
     1201 cgtgctaaag gggcggtagc tgaggttaat aagattgcca gtgtctacgg aaaggacaac
     1261 aggatcgctt tgcaggaaca gttggatact tccttgctgt atctgaataa agaaaaagcc
     1321 gctgactggc ttctttcagc cgggctccaa ttgcccgggg cgaacaccat caacggctct
     1381 tctggattta aagtactaca tcctgaagat attcgcaagg gggcgtatta ctcccgcacc
     1441 agcagtctga caccggaaga gaccattgca tc
//
LOCUS       DAZKHK010000350         1467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKHK010000350 DAZKHK010000000
VERSION     DAZKHK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.352"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..930
                     /locus_tag="ACHOS5_005406"
     CDS             <1..930
                     /locus_tag="ACHOS5_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease PIN"
                     /protein_id="HFP9780038.1"
                     /translation="AESYCNLLWANNTLPSASVNVSFDGNTSGIFPLNLQAGGLSTVI
                     KRAAKNMDTFVTLDGTYRYWIQYPEAWQTTPDGLKYRITSELEVSGTQTAGVKTVVTP
                     VGYHTWVNTYGCRDVGGTYDFGVASVSGVNIEIDRGTAWPGVYSIQLPVKVAYEENKG
                     NYDGKNGGGWQEFPVSMKSFSPVDSKGISITISSKCNVGEQSLSVNMGDNITPDEAKS
                     GVEKKVNFSLTCNAPAKVSLSLKGTDIVDGVNNKTKCGSGSCSLNFDNDSSSKILEVN
                     QGTYQVPITVRFQDANPVAGGFDGSAVLSVDIL"
     gene            983..>1467
                     /locus_tag="ACHOS5_005407"
     CDS             983..>1467
                     /locus_tag="ACHOS5_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9780039.1"
                     /translation="MFNDIRGDVTSGSDSLLQAIQKREFKPYIQPIVCASNHKIIGGE
                     LLVRWHTSHRGVIYPDSFIDNMEKAGILSELTCSIINEVVVNFDGENKFNGEDFILHV
                     NVAPSLLHNKKFISTCINAAKKNNLSLVLELTEQYFFTVDSKTISTFHCLKESGVQFA
                     L"
BASE COUNT          453 a          244 c          330 g          440 t
ORIGIN      
        1 gctgaaagct actgtaacct cctttgggcg aataatactc taccaagtgc ctcagtcaat
       61 gtcagttttg atggcaatac atccgggatt tttcctctca atttacaggc gggtggcctg
      121 tcaactgtaa taaaaagagc tgcaaaaaat atggatactt ttgtaacgct tgatggaact
      181 tatcgatact ggatacagta tccggaagca tggcagacaa cccctgacgg actaaaatat
      241 cgaataacca gcgaactgga ggtttcgggc actcaaactg caggtgtcaa aacagttgta
      301 acgcctgtag gttaccatac atgggtaaat acatacggat gcagggatgt cgggggaact
      361 tatgattttg gggttgcatc tgtcagtggg gtgaatattg aaatagacag gggcactgca
      421 tggccggggg tttattctat acagttacca gtaaaagttg catatgaaga gaataaaggg
      481 aattatgacg gtaaaaatgg cggaggctgg caagaatttc ctgtatccat gaaatctttt
      541 tctccggtag actctaaagg catcagcata acgataagct caaagtgtaa tgttggtgaa
      601 caaagtttga gtgttaatat gggtgataat attactccgg atgaagcaaa atcaggtgtg
      661 gagaaaaagg ttaatttttc tcttacttgt aatgccccgg caaaggtatc actatctctt
      721 aaaggtaccg atatcgtcga tggggtcaat aacaaaacaa aatgtggttc tggtagctgc
      781 tctctgaatt ttgataatga tagttcatct aaaattctgg aagtaaatca aggaacatat
      841 caggtgccga ttacagtacg ttttcaggac gcaaaccccg ttgccggagg gtttgatggt
      901 agtgctgtat tatccgttga tattctataa tatatattga tgtagtcttg ctgattaaca
      961 aataaataaa ggagtacttc aagtgtttaa tgatatcaga ggtgacgtca catcgggttc
     1021 tgatagttta ttgcaggcaa tacagaaacg agaattcaaa ccatacattc agcctattgt
     1081 ttgtgccagc aaccataaaa ttattggtgg cgagttgctt gtccgttggc atacatctca
     1141 tagaggagta atttatccag atagttttat agataatatg gagaaagctg ggattctgtc
     1201 tgagcttact tgttctataa tcaatgaggt ggttgttaat tttgatggtg agaataaatt
     1261 taatggtgag gattttattc ttcacgtaaa tgtcgctcca tctctattgc ataataaaaa
     1321 attcatatcc acctgtataa acgctgcaaa aaaaaacaac ctgtcattag ttcttgaatt
     1381 aactgaacag tattttttta ctgtggatag caaaaccata tccacctttc attgtctaaa
     1441 ggagtcgggt gtgcaatttg ctttaga
//
LOCUS       DAZKHK010000351         1444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKHK010000351 DAZKHK010000000
VERSION     DAZKHK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.354"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1444
                     /locus_tag="ACHOS5_005408"
     rRNA            <1..1444
                     /locus_tag="ACHOS5_005408"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          360 a          333 c          460 g          291 t
ORIGIN      
        1 acgagtggcg gacgggtgag taatgtctgg gaaactgcct gatggagggg gataactact
       61 ggaaacggta gctaataccg cataacgtcg caagaccaaa gagggggacc ttcgggcctc
      121 ttgccatcgg atgtgcccag atgggattag ctagtaggtg gggtaacggc tcacctaggc
      181 gacgatccct agctggtctg agaggatgac cagccacact ggaactgaga cacggtccag
      241 actcctacgg gaggcagcag tggggaatat tgcacaatgg gcgcaagcct gatgcagcca
      301 tgccgcgtgt atgaagaagg ccttcgggtt gtaaagtact ttcagcgggg aggaagggag
      361 taaagttaat acctttgctc attgacgtta cccgcagaag aagcaccggc taactccgtg
      421 ccagcagccg cggtaatacg gagggtgcaa gcgttaatcg gaattactgg gcgtaaagcg
      481 cacgcaggcg gtttgttaag tcagatgtga aatccccggg ctcaacctgg gaactgcatc
      541 tgatactggc aagcttgagt ctcgtagagg ggggtagaat tccaggtgta gcggtgaaat
      601 gcgtagagat ctggaggaat accggtggcg aaggcggccc cctggacgaa gactgacgct
      661 caggtgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac
      721 gatgtcgact tggaggttgt gcccttgagg cgtggcttcc ggagctaacg cgttaagtcg
      781 accgcctggg gagtacggcc gcaaggttaa aactcaaatg aattgacggg ggcccgcaca
      841 agcggtggag catgtggttt aattcgatgc aacgcgaaga accttacctg gtcttgacat
      901 ccacggaagt tttcagagat gagaatgtgc cttcgggaac cgtgagacag gtgctgcatg
      961 gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta
     1021 tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga taaactggag
     1081 gaaggtgggg atgacgtcaa gtcatcatgg cccttacgac cagggctaca cacgtgctac
     1141 aatggcgcat acaaagagaa gcgacctcgc gagagcaagc ggacctcata aagtgcgtcg
     1201 tagtccggat tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtgga
     1261 tcagaatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg
     1321 agtgggttgc aaaagaagta ggtagcttaa ccttcgggag ggcgcttacc actttgtgat
     1381 tcatgactgg ggtgaagtcg taacaaggta accgtagggg aacctgcggt tggatcacct
     1441 cctt
//
LOCUS       DAZKHK010000352         1442 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKHK010000352 DAZKHK010000000
VERSION     DAZKHK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.355"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1442
                     /locus_tag="ACHOS5_005409"
     CDS             <1..>1442
                     /locus_tag="ACHOS5_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9780040.1"
                     /translation="IVGLQVDAEQFGGQQLTVNYHIRGRIIQVPSNYDPEKRTYSGIW
                     DGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGG
                     TEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDV
                     VVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMD
                     AFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTG
                     GRILSMDAASRTLTLDREVTLPEAGTSTVNLINGSGKPVRVDITAHPAPDRIQVSVLP
                     DGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGATFEPL
                     SGSLNSVIPPAVQHLTVEVSAADGQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLV
                     TSAITADTEHRFSGLPLGEYTLTVRAINSYG"
BASE COUNT          291 a          406 c          482 g          263 t
ORIGIN      
        1 attgtggggc tgcaggtgga tgcggagcag tttggtggcc agcagctgac ggtgaactac
       61 catatccgtg gtcgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcacctac
      121 agcggtatct gggacgggag tctgaaaccg gcatacagca ataacccggc ctggtgcctg
      181 tgggacatgc tgacccaccc gcgctacggg atgggaaaac gcctgggggc ggcggatgtg
      241 gacaagtggg ctctgtatgc catcgggcag tactgcgacc agacggtccc ggatggtttc
      301 gggggcacag agccgcggat gacctttaat gcgtacctgt cacagcagcg taaggtgtgg
      361 gatgtcctgg gggatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg
      421 ctgacgttcg ttcaggaccg tccgtcggat gtggtgtggc cctacaccaa cagcgatgtg
      481 gtggtggatg ataacggcgt ggggttccgc tacagcttca gtgccctgaa ggaccggcac
      541 acggcggtgg aggtgaatta cactgacccg cagaacggct ggcagacttc cacggaactg
      601 gtggaagacc cggacgccat cctgcgctac gggcgcaatc tgctgaagat ggatgcgttc
      661 ggctgtacca gccgcggtca ggcccaccgt gccggactgt gggtgataaa gacagaactg
      721 ctggaaacgc agacggtgga tttcacgctt gggtcacagg ggctgcggca cacgcccggt
      781 gacatcattg aaatctgtga taacgactat gccgggaccc tgaccggcgg acgtatcctg
      841 tccatggatg ctgccagccg taccctgacg ctggaccgtg aggtgacact gccggaagca
      901 gggacatcga cggtgaacct gattaacggc agcggtaagc cggtgcgcgt ggacatcact
      961 gcacaccccg cccctgaccg gatacaggtc agcgtcctgc ctgatggcgt ggagacatac
     1021 ggtgtgtggg gactctccct gccgtcactg cgtcgtcgcc tgttccgctg tgtttccatc
     1081 cgggaaaaca cggacggcac ctttgccatc acggcagtgc agcacgtacc ggaaaaagaa
     1141 gccatcgtgg ataacggggc cacctttgag ccgctctccg gttcactgaa cagcgtcatc
     1201 ccgccggcag tgcagcacct cacggtggag gtgagcgcgg ctgacggcca gtatctggcg
     1261 ctggcgaaat gggacacgcc gcgggtggtg aagggcgtgc gcttcagtct gcgcctgacc
     1321 agtggcagtg gtgaaaacag ccgcctggtg accagcgcca tcactgccga cacggagcac
     1381 cgtttcagtg gcctgccgct cggggaatac accctgacgg tcagggcgat aaacagctat
     1441 gg
//
LOCUS       DAZKHK010000353         1426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKHK010000353 DAZKHK010000000
VERSION     DAZKHK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.356"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            222..851
                     /locus_tag="ACHOS5_005410"
     CDS             222..851
                     /locus_tag="ACHOS5_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9780041.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
     gene            complement(1033..>1426)
                     /locus_tag="ACHOS5_005411"
     CDS             complement(1033..>1426)
                     /locus_tag="ACHOS5_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HFP9780042.1"
                     /translation="VQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPY
                     ATCFDSEGKKKYSRRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALY
                     VAFTRATRELLVTCYRENSISRHLEDFV"
BASE COUNT          521 a          297 c          249 g          359 t
ORIGIN      
        1 tttcttttca taaactcttt aatattcaaa agaaaatatt atacccagca ttatttattt
       61 gaaaatcaca tcgtttatca tgaggagcat attatctcct ccagcaccaa aaataaaaac
      121 acaaaacaac cttacaactt atatattaac acaaagtaga gaaagaagaa caactcatct
      181 taaaacaaga aaaatataca aattaaaaga aatagaaaca catgagcatt gaacaaatca
      241 taaatgaaat aaatgtatac cttaattctc ttaagaaaga agatgaacgc attgatgcaa
      301 ttaataaaat aaagattgag ttacatagga gtagtccttt ttacgcagaa cctgttgatt
      361 gtgttatatg gataaaaaac aatctaattg aagcaaacga ctataatcct aacgtcatgg
      421 ctccctccga aaaaaggtta ttaacacact ccctgttggt tgatggattc actcaacctg
      481 ttgtcacctt agctcaaaag gggaaataca ccgttattga cggatttcac agacaaatgc
      541 ttgggaaaag cacaacaaaa ctagagaaaa aactaaaagg atatttacct gtcacatgca
      601 ttaagcatga taataacttt aaagcttact ctatcgcctc gaccatcagg cataacaggg
      661 ccagaggaaa gcaccagata gacgcgctct ccgatatcgt ccgagatctt tcccggatgg
      721 gatgggatga tctccgaata ggtagtgaac tgggcatgga tacagatgag gtactgagat
      781 taaaacaaat aagtgggtta acagaactat ttgaggagga aaactttagt ccggcatgga
      841 caataaagtg atcatttagg gggtgacttt atccagtaat taccgactga tatatgatca
      901 ccccatgtaa tggacagcta ttgacagatc aaaaacatgg gggatattaa ttcaactctg
      961 aatactcgct aaatacccat cttagattat caaaaaacga atgattacat atcagcaata
     1021 ataaaacaca acttatacaa aatcctcaag atgacgactg atactgtttt cacgataaca
     1081 ggtcaccagt aattcccgtg tagcccgggt gaacgcaaca taaagcgccg gaataacttc
     1141 agactcatcc gcagcacctg agtgaacgaa agaagcattg ataaccgcaa catacggaaa
     1201 ctccagtccc ttactgctct ggtaggtcag taaatgcaca acatccttgc gtcgcgaata
     1261 tttcttctta ccttcactgt caaagcaggt ggcataaggt atcccgcgtt gtgtcatgac
     1321 ctctttcaga tgtttcactg aaaactctgc aggacacaaa acagccatat cactccagtg
     1381 accgcacagt gtatagcgtt tctccagcca gcccacaacc tgcact
//
LOCUS       DAZKHK010000354         1423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKHK010000354 DAZKHK010000000
VERSION     DAZKHK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.357"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..431
                     /locus_tag="ACHOS5_005412"
     CDS             <1..431
                     /locus_tag="ACHOS5_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9780043.1"
                     /translation="QVCRMYNNAFVGPERNNHGHAVILKLRELYPTRYIYNEQHLDQA
                     YDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWSGTLSEMNTYVYDAKGSMNAQ
                     EGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWMAH"
     gene            431..>1423
                     /locus_tag="ACHOS5_005413"
     CDS             431..>1423
                     /locus_tag="ACHOS5_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9780044.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLV"
BASE COUNT          362 a          331 c          410 g          320 t
ORIGIN      
        1 cgcaggtctg tcgtatgtat aacaacgcgt ttgtggggcc ggagcgtaat aatcacggac
       61 atgcagttat cctgaaactc cgggaactct atccgacacg ttatatctac aacgaacagc
      121 atcttgacca ggcatatgac gacgatacgc cccgccttgg ctggctgaca acccgtcaga
      181 gcaaacctgt tctgaccgaa ggaatgaaaa cgctcctgaa taatggaata tcagggatcc
      241 gctggtcagg cacattatcg gaaatgaaca cctacgttta tgacgcgaaa ggctccatga
      301 atgcacagga aggctgcttt gatgatcagc tcatgagcta catgattgcc caggagatgc
      361 gcgccagaat gccggtgagg gtaaaacaga aaacggataa acgcagaacc acacactgga
      421 tggcacactg atgaaaaatg aaattaacac cacagcgatg aaaaacgatc atggatccac
      481 gccgcgtttt tctcagcgtc agttactgtc tctctgttct gatattgaca gtcagcctct
      541 ctggcgtgat gccgcaaaca aggcctgtgc gtattatgat ggcgaccagc tggcaccgga
      601 agttatccag gtactgaaag atcgcggtca gcccatgacc atccataacc tcattgcccc
      661 cacggtagat ggtgtactgg gaatggaggc aaaaacaaga acggacctga tagtgatgtc
      721 agacgatccg aacgatgaaa cagagaaact ggcagaggcc attaatgcgg agtttgctga
      781 tgcgtgccgt cttggcaaca tgaataaggc ccgctccgat gcctatgcgg aacaaatcaa
      841 ggcggggctc agttgggtgg aggtcaggcg caacagcgat ccgttcggac ctgaattcag
      901 ggtatccaca gtcagcagaa atgaggtttt ctgggactgg ctcagccggg aatccgacct
      961 gagtgactgc cggtggctga tgcgtcgccg ctggatggat accgatgagg caaaagccac
     1021 gttcccggga atggctcagg ttatcgatta tgctattgat gactggcgtg gttttgtcga
     1081 taccacgatt actgaaggcc agcccagtcc gttgatgagt gcatgggaag agtatcagtc
     1141 atgggatcgc agggagaatg aatggcttca gcgtgaacgc cgtcgtgtgc tgcttcaggt
     1201 ggtttattac cgtacattcg aacgtcttcc ggtgattgaa ctcagtaatg gacgggtggt
     1261 ggcttttgat aaaaataatc tgatgcaggc ggtggctgtg gcatccgggc gggttcaggt
     1321 gaaagtcggg cgggtaagtc gtattcgtga agcctggttt gtgggcccac actttattgt
     1381 ggatcgcccc tgtagtgctc cgcaggggat gtttccgctg gtt
//
LOCUS       DAZKHK010000355         1419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKHK010000355 DAZKHK010000000
VERSION     DAZKHK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.358"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..673
                     /gene="comR"
                     /locus_tag="ACHOS5_005414"
     CDS             41..673
                     /gene="comR"
                     /locus_tag="ACHOS5_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP9780045.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPPHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(735..1274)
                     /gene="ycfJ"
                     /locus_tag="ACHOS5_005415"
     CDS             complement(735..1274)
                     /gene="ycfJ"
                     /locus_tag="ACHOS5_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9780046.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
BASE COUNT          360 a          390 c          343 g          326 t
ORIGIN      
        1 taataacctt tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca
       61 atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa
      121 ggccatgaaa ttgttctggc agcacggtta tgaagcgact tctcttgcgg acctcgttga
      181 agcgaccgga gctaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt
      241 ccgcgccgta ctcgaccgct atatcgatcg ttttgccgcc aagcatgaag cacagctgtt
      301 ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg
      361 ctttaccagc aaagacactc cggctggctg cttcatgatc aacaactgca ccaccctctc
      421 cccggattct ggagatatcg ccaatacgct gaaatcacgc catgcgatgc aagagcgcac
      481 tttgcagcag tttttatgtc aacgacaagc gcgcggggaa atcccgcccc actgtgacgt
      541 gacgcatctg gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga
      601 aggtgcatcg ctggaaaaac tgatgcagat tgcccgaacg actttgcgtt tatggcccga
      661 actgctgaaa taagtcgcaa aaaaagcccc tcagcttgag cgaatgaggg gctaaattgc
      721 agagtacaac cttgttatgc tttgttattc aaaatcagct gcccgttgct atctagcggg
      781 atctgcgtac ccggatcgcg gtccattcgg attttgccct gctgatcgcc aatcttatag
      841 gtcacatcat aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc
      901 gtagtcgtgt aagtatcgct ttcctggaga gagccctgga tctggttacc tgcatatcca
      961 ccccccagcg cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc
     1021 ccaatcacgc cgccagcaac agcgccgagt accgacccgg taatgcgatt ttcatcctgc
     1081 accggtcgac gatgggtcac tgtaacgttg cgacactcct gacgcggtgt tttaaccgtt
     1141 tccttgattg gggttgcaga aacaacctga gcgtattgcg ggccacgttc aaacacgttc
     1201 agactggcca ctgccgctac gcccagcgca gctgcgacac caatcccgat acccgccaac
     1261 attgacttat tcacgggaat acctccttct ttgctgttat gccaatcatg caagcgaagg
     1321 agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa tggctggaaa tacttaggga
     1381 ttcggaggag tctgaaggaa tgcctgatgc gacgcttaa
//
LOCUS       DAZKHK010000356         1398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKHK010000356 DAZKHK010000000
VERSION     DAZKHK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.359"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..470
                     /locus_tag="ACHOS5_005416"
     CDS             <1..470
                     /locus_tag="ACHOS5_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780047.1"
                     /translation="QQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVAVSIDGK
                     LQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKVKTAEQE
                     QAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKLG"
     gene            494..>1398
                     /locus_tag="ACHOS5_005417"
     CDS             494..>1398
                     /locus_tag="ACHOS5_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP9780048.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLFGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAG"
BASE COUNT          356 a          335 c          391 g          316 t
ORIGIN      
        1 cacagcaggc agaaggtaat ggtggtgagg atttatcacc ggttcttgat gcgatgaatg
       61 ccgtgccggt gctgaaaacg tggcaggagt ccgatccaga tcgcttctcg gttgctgtat
      121 ccatcgacgg gaagctccag aatgaccccg catggaaaga caaaacgctc actgaacgtt
      181 tcgctgaagt ggcccgtcgt acgcaggttg ctttcggtga agtcagtgag tcgtctgctg
      241 acaacaaggc agacaaaacg gatatccgga aaacggcgga agagaaagtg aagaccgctg
      301 aacaggagca ggcagtacct gcttccccgt cagatttagg caccacggct tccgtcggaa
      361 ccggtgataa ttttgaacgg ttacttggcg cttctcattc agaggcagag gcgattatgc
      421 gcggtatgac gaatgctgaa atagacgcgc ttctggagaa gctcgggtaa cttactgaag
      481 gagtactgaa gtaatgacga ctgtaacatc agcccaagcg aataagctgt atcaggtggc
      541 gctttttacc gctgccaacc gcaaccgctc gatggtcaat atcctcactg aacagcagga
      601 agcgccaaaa gcggtttcgc cggacaagaa aagcacgaag cagaccagcg cgggtgcgcc
      661 ggttgtccgt atcacagacc ttaacaaaca ggccggtgat gaagtgacct tcagcatcat
      721 gcacaaactc tcaaaacgtc cgacgatggg agatgagcgt gttgaaggtc gtggtgagga
      781 tctcagccat gctgacttct ccctgaaaat caatcaggga cgtcacctgg tggatgcagg
      841 tggacgtatg agtcagcagc gcacgaagtt taacctggca tcctcagcca gaacacttct
      901 ggggacgtac tttaatgacc tgcaggacca gtgtgcgata gtgcatcttg ctggtgctcg
      961 cggtgatttt gttgctgacg acaccattct gccgacagcg gagcaccctg aattcaaaaa
     1021 aatcatgatc aacgatgtac tgcctccgac acatgaccgt cacttttttg gcggtgatgc
     1081 gacaagcttt gagcagattg aagcggcaga tattttttct attggcctgg tggacaatct
     1141 ctccctgttc attgacgaaa tggcgcatcc gttacagccg gttcgtctgt tcggtgatga
     1201 acttcacgga gaagatccat attacgtcct gtacgtcacg ccgcgtcagt ggaatgactg
     1261 gtacacctcg acgtccggta aggactggaa ccagatgatg gttcgtgccg tgaaccgtgc
     1321 aaaaggtttt aatcatccgc tgttcaaagg tgaatgtgcg atgtggcgca atatactggt
     1381 tcgtaagtat gcgggtat
//
LOCUS       DAZKHK010000357         1396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKHK010000357 DAZKHK010000000
VERSION     DAZKHK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.360"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..360)
                     /locus_tag="ACHOS5_005418"
     CDS             complement(<1..360)
                     /locus_tag="ACHOS5_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9780049.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEE"
     gene            complement(416..748)
                     /locus_tag="ACHOS5_005419"
     CDS             complement(416..748)
                     /locus_tag="ACHOS5_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9780050.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(758..>1396)
                     /locus_tag="ACHOS5_005420"
     CDS             complement(758..>1396)
                     /locus_tag="ACHOS5_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9780051.1"
                     /translation="PDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSG
                     QEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTD
                     VVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMT
                     VETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
BASE COUNT          282 a          405 c          400 g          309 t
ORIGIN      
        1 ctcttcgtca cgcatgttct gcaggatgat gcgacggcgg cggtaagccg ggtccgccag
       61 attctgcgga tcttcatccg gcaggcgacg cagggtcatc tgcggattca cttcatgctt
      121 gggtttgaca tatcccggcg taaattcaga ggtggagccg ccacgggaac ggataacctc
      181 accggaaaca atcggcgaaa cgtacagcgc catgtttacc agtcccggaa tttgtgagag
      241 atagactttc tccgtagtga agggatagct ctcacggaaa aagagacgca gaaacagcgg
      301 atcaaactta aatttctgct catttgccgc cagcaactgg gcggttgtgt acatcgacat
      361 aaaaaaatcc cgtaaaaaaa gccgcacagg cggcctttag tgatgaaggg taaagttaaa
      421 cgatgctgat tgccgttccg gcaaacgcgg tccgtttttt cgtctcgtcg ctggcagcct
      481 ccggccagag cacatcctca taacggaacg tgccggactt gtagaacgtc agtgtggtgc
      541 tggtctggtc agcagcaacc gcaagaatgc caacggcagc accgtcggtg gtgccatccc
      601 acgcaaccag cttacgggtg gaggtgtcca gcatcagcgg ggtcattgca ggcgctttcg
      661 cactcaatcc gccgggcgcg gttgcggtat gagccgggtc actattgccc agcggctggt
      721 aatgggtaaa ggtttctttg ctcgtcataa acatccctta cactggtgtg ttcagcaaat
      781 cgttaacggc atcagatgcc gggttacctg cagccagcgg tgccggtgcc ccctgcatca
      841 gacgatccag cgcagtgtca ctgcgcgcct gtgcactctg tggtgctgcg gccagaatac
      901 ggcgggccgt ttccacggtc ataccggggg tttctgccag cacgcgtgcc tgttcttcgc
      961 gtccgtgagc ctcctcacag ttgaggatcc ccataatgcg gctgttttct gccgcaaccg
     1021 ctgcggtgat ctgcgcgttc acgtccggct gcgccgcgct ggcgttctcg ccctccgtcg
     1081 ctggcaccac gtcagtaacg tcagcctgcg aagcagtggc tgaaacagtt gttgattgag
     1141 tctctttggt cattcgccct cctgagagac gggatttacg tgcatccagt gcatcacgca
     1201 tgacggtgat cgcatcggtg ctgttaacaa gttcatcagc cagtccggca tcaatggcct
     1261 cctgaccgct gtacactgca gcctcggtat ccagcacagc ctgcacggac aggccggtat
     1321 atgccgacac cttctgcgca aacatctggc gggttgcgtc catccgggac tgcagtgtct
     1381 cccggacgtc atccgg
//
LOCUS       DAZKHK010000358         1384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKHK010000358 DAZKHK010000000
VERSION     DAZKHK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.361"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..554
                     /locus_tag="ACHOS5_005421"
     CDS             177..554
                     /locus_tag="ACHOS5_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFP9780052.1"
                     /translation="MATCTENSSGAACQKAMNERDAVGLALATGTVALLPGSAQVMWG
                     LGASANAGISYLADGTVDPANAAIAGWGNVFSMGNGLAGTVGWNAAGGALGNWIGNKD
                     PLSGAISNGIGSAAGYGIGKGIT"
     gene            591..782
                     /locus_tag="ACHOS5_005422"
     CDS             591..782
                     /locus_tag="ACHOS5_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase"
                     /protein_id="HFP9780053.1"
                     /translation="MTNGWDPKYDPTWIKYAEVNRNDIIREGMKPSNIPGTAGDIGGS
                     IGSELGGKVTDKVSEELKK"
     gene            1052..1294
                     /locus_tag="ACHOS5_005423"
     CDS             1052..1294
                     /locus_tag="ACHOS5_005423"
                     /inference="COORDINATES: protein motif:HMM:NF044555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6862 domain-containing protein"
                     /protein_id="HFP9780054.1"
                     /translation="MSEKTELEIAKQTLKNSKDPAEREKVQQKYDALLEKDIASDKEV
                     IAACSNGNAGSSACASARLKVIASKEGYEDGPYKRL"
BASE COUNT          395 a          213 c          380 g          396 t
ORIGIN      
        1 gagagctttt tttcaaccta aaaataaaac taaggttaat atttttatgg ttgtcacgat
       61 tattattcat catattgata ttaaatcgtt tttatcgttg atttgttgtt gagaataatt
      121 tacttggcgg tagtgaatat gctcatgctg cgtggatacg ccagcatggt atcgacatgg
      181 cgacatgtac agaaaactct tccggtgccg cctgccagaa agcaatgaat gagagggatg
      241 ctgtagggct ggctttggca acaggtactg tggcactact ccccggtagt gctcaggtaa
      301 tgtggggact tggagcaagt gcgaatgctg gtatcagtta tctggctgat ggtactgtag
      361 atccggcaaa cgcagccatt gcaggctggg ggaatgtgtt cagcatgggt aatggcctgg
      421 cggggactgt gggctggaat gctgcaggtg gtgcgctggg taactggatc ggcaacaaag
      481 atcctctgtc tggtgcaatt tcaaatggta tcggttctgc tgctgggtat ggtatcggta
      541 aaggtattac ttaggggaca gataagatta cgaatgctgt aggaaaatgg gtgacaaatg
      601 gctgggatcc taagtacgat ccaacatgga taaaatatgc tgaagtaaat cgtaacgata
      661 tcattcgaga aggcatgaaa ccgagcaata ttcctggaac tgctggagat attggcggtt
      721 ctataggatc cgagctaggt ggaaaagtaa cagataaagt atctgaggaa ctcaagaaat
      781 gaaatattct tttcgagtgg taataggtaa gtttattata tgcagttcaa ttctcttatt
      841 gtgttttatt ggcatgatct tagcggccat aataactaaa ctaacgtttg tagcgttagc
      901 atggtttcaa agcgggattt ttgaagtttt gtggagcgat attgtttatg ctgctcagtt
      961 gggtggtctg gggggggcat attaggtttt tgttgggttt tgttatatct ttttagggca
     1021 aagggcttta gttgagaata actacctgag cgtgtctgaa aagacagaac ttgagatagc
     1081 aaaacagacg cttaaaaaca gtaaagatcc ggcagaacgt gaaaaggtac agcagaaata
     1141 tgatgcattg cttgaaaaag atattgccag tgataaggag gttattgctg cctgcagtaa
     1201 tggtaatgcg gggagttcag cgtgtgctag cgcaaggctg aaagttattg cgtcgaaaga
     1261 agggtacgaa gatggccctt ataagagact gtaattaaaa ttgtgtaatt gcctgttttt
     1321 gatatgttca ctccaacaac ggagacaggc aaacgatgga cgaaaagaaa cttaaagccc
     1381 ttgc
//
LOCUS       DAZKHK010000359         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKHK010000359 DAZKHK010000000
VERSION     DAZKHK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.362"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(34..>1364)
                     /locus_tag="ACHOS5_005424"
     CDS             complement(34..>1364)
                     /locus_tag="ACHOS5_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 25 domain-containing
                     protein"
                     /protein_id="HFP9780055.1"
                     /translation="AEGTTQAAVAEGTITIRDRDNQKQNLANLSRDPAHANDSISPIF
                     DKEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQRQEY
                     LAKLRDTQAYRNEMAKYGTGSEIQRGIQAATAALQGLAGGNLAGALAGASAPELAHLL
                     KSTEKDPAVNAIAHAILGGAVAAMQGNNVAAGAAGAATGELAARAITGMLYPGVKQSD
                     LSEEQKQTISTLATVSAGLACGLTGNSTASAAVGAQSGKNAVENNSLSDGWNNILPSG
                     TQDYGQAVASWNQYAQDNNLTPEQVQEGMNRIAIGEGPFWGTTYKVHPVVQAGGDVSF
                     IRGYTLSGTIDDNHISVNQGDIYSIGAHGGASIGLSFGPYFPGLINSNDNDYSINGGF
                     GVGAVGLSTGKDGVSFTFGFGPSWGWSATEIKGVDVNGTSTSEVYRYDFK"
BASE COUNT          304 a          405 c          281 g          374 t
ORIGIN      
        1 gcaagcatca ataaaaatag taaactataa aaattattta aaatcatatc gataaacctc
       61 acttgttgaa gtgccattaa cgtcaacgcc tttaatttca gttgcactcc aaccccaact
      121 tgggccaaaa ccaaaagtga aactaacacc atctttaccc gtagaaagcc ccactgcacc
      181 aacgccaaaa ccaccattta tcgaataatc gttatcattt gaatttatta atcccgggaa
      241 atacgggcca aaggataatc cgatcgaggc tccgccatga gcacctatac tataaatatc
      301 cccttgatta actgaaatat gattgtcatc tatagttccg cttaaagtat aaccccggat
      361 aaagctaaca tcaccgccag cctgaactac cgggtgtact ttatatgttg tcccccaaaa
      421 aggcccttca ccaatagcta tacgattcat cccttcttga acctgctccg gtgtcagatt
      481 attgtcctga gcatactgat tccacgaggc tacggcctgt ccgtaatcct gtgttcctga
      541 tggcaggata ttgttccagc catcactgag tgagttattc tcaacagcat tcttcccgct
      601 ttgtgctccc actgctgctg atgcggtgct gtttccggta agaccgcagg ccagccctgc
      661 tgacacagtt gccagcgtac ttatcgtctg cttctgctct tcactgagat ctgactgctt
      721 cacgccagga tacagcattc cggtaatcgc tctggctgcc agctctcccg ttgctgcgcc
      781 tgctgcaccg gcagcgacat tattaccctg cattgccgcc actgcgccgc ccagaatggc
      841 gtgagcaatg gcgtttactg ccgggtcttt ttctgttgat ttcagcagat gtgccagttc
      901 cggtgctgaa gctccggcca gagcacctgc taaattaccc cccgccagcc cctgaagcgc
      961 cgctgtcgcg gcctggatac cccgctgaat ttcactgccg gtaccatact tcgccatttc
     1021 attcctgtaa gcctgcgtgt cacgcagttt cgcaagatat tcctgacgtt gtttctctgt
     1081 tgcattagca ggaagtgttt cgccggttgc ttccttcgcg gccttcaacg cattcagttc
     1141 tccctgtgta cgcgcaatat ccgccacctg actgcctatg tcactgataa gccccactgt
     1201 ctgcagacgc ctctgctctt tctccttgtc aaatatcggg ctgatactgt cattagcgtg
     1261 cgcagggtca cggctcaggt tcgccagatt ctgcttctga ttgtccctgt cccggatggt
     1321 gatagtgcct tctgccactg cggcctgagt cgttccttcc gcat
//
LOCUS       DAZKHK010000360         1358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKHK010000360 DAZKHK010000000
VERSION     DAZKHK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.363"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..454
                     /locus_tag="ACHOS5_005425"
     CDS             <1..454
                     /locus_tag="ACHOS5_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780056.1"
                     /translation="NYDYNAAQRNREIVDSYQQANEARLNSQQAQFEASMANDRVSRI
                     QVQLNNTINSHKKAIADYEQRLEEQKAISFKLIMKVNIFERTLNRLQEQWPEKKESIL
                     DEIQHQKDYCSVEEYKEKWWKWVNDGGLTPEANCLKFPYPEREIKNKT"
     gene            566..1000
                     /locus_tag="ACHOS5_005426"
     CDS             566..1000
                     /locus_tag="ACHOS5_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780057.1"
                     /translation="MTIVSNPRYVVQLIVLPKYLMSNIAPSEVIKGKIDKSEMIYMYI
                     IYDTVGLTYKNSFPDDEKAAILECYKLNYSNIPIYKAEVAKFKDGSPDKLKDISPYQK
                     IEDSNEEYDNLHDEEKVKLFEYLEKEHGVENISPVKLRRPRP"
     gene            1070..1267
                     /locus_tag="ACHOS5_005427"
     CDS             1070..1267
                     /locus_tag="ACHOS5_005427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780058.1"
                     /translation="MAKPKYSLETRLAAVNHYLAGHDGARRIAERFGVEETSLRKRDK
                     FSPRPCHIYAPSIGGNFPVII"
BASE COUNT          495 a          251 c          243 g          369 t
ORIGIN      
        1 gaattatgat tataatgcag cacaacgcaa tagagagata gtagattcat atcaacaggc
       61 taatgaagcc agactgaact ctcagcaagc tcagtttgaa gcatctatgg ctaatgacag
      121 ggttagtaga atacaggtgc agttgaataa tacaatcaac agtcataaaa aggctattgc
      181 tgattatgag cagcgtttgg aagaacaaaa agcaatttct tttaaattga tcatgaaggt
      241 aaatatattt gagagaacgt taaatcgcct tcaagagcaa tggcctgaaa aaaaagaaag
      301 catcttagac gaaattcagc accagaaaga ttactgttcg gttgaagaat acaaagagaa
      361 atggtggaaa tgggtcaatg atggtggcct tacaccagaa gctaattgct taaaattccc
      421 ttacccagag cgcgagataa agaacaagac ataagaagaa actaagtatt ttttcataac
      481 attcaattaa atttcaataa gacacttctc tactgtgtct cctacaaata cgtctaaaat
      541 tcactcacct tatggaacgt tcaatatgac catcgtttct aatcctcggt atgttgtaca
      601 gctaatcgtc cttccaaaat atcttatgag caatattgct cccagtgaag ttattaaagg
      661 aaaaatagat aaatcagaaa tgatctatat gtatattatt tacgacactg taggtttaac
      721 atacaagaac agctttcctg atgatgaaaa agccgctatt ctggaatgct ataagttgaa
      781 ctacagtaat attcccattt acaaggcaga agtagccaaa ttcaaagacg gctcacccga
      841 taaactcaag gacatatccc cataccaaaa aattgaagat tctaatgagg aatacgacaa
      901 ccttcacgac gaggaaaaag tgaaattatt cgaatattta gagaaagagc atggtgtaga
      961 aaatatctct ccagttaagc taagaagacc gagaccttaa aaataagcct cagcataatg
     1021 gactgcgccc caaaagttgg acacccaact gattaaggtg cagttttttg tggcaaagcc
     1081 aaaatattct ctcgaaacca gattagctgc cgttaatcat tatcttgccg gacacgatgg
     1141 cgcacgtcgt atcgctgaac gttttggtgt tgaagaaaca tcattgcgca aaagagataa
     1201 gtttagccca cgaccatgcc atatatacgc tccatcaata ggtggcaact ttcctgtaat
     1261 catttgatgt atttctttcg aagtgccttt tttaaatata tctttagaac aaaaataact
     1321 cattatttac gctctttaca aaataacttt aaaatctt
//
LOCUS       DAZKHK010000361         1353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKHK010000361 DAZKHK010000000
VERSION     DAZKHK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.364"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1353
                     /locus_tag="ACHOS5_005428"
     CDS             <1..>1353
                     /locus_tag="ACHOS5_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780059.1"
                     /translation="TGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIAD
                     ADPMEQPAFMRDPRFRGFTGDDTEVQARLARGNAPMAEELVRSQMAEGDAGPTAQELT
                     ERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAPAPE
                     NAAGRQGETLEGEIVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRD
                     SLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVFTDRESTVRGGEVRGKKIEDFGE
                     EIKGAAKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIAL
                     YRSDIPAKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQL
                     LRTLPPSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDEL
                     L"
BASE COUNT          345 a          346 c          414 g          248 t
ORIGIN      
        1 accggcctga aaccgtccgt agtgaagaaa ggcgagaatc agtatgccgt tgaaatggat
       61 aatcctgcgt tctctgaaga tgtggcaacg gaaaccatta acaccctggc tgacggagag
      121 cgtattgctg atgctgaccc gatggagcag cccgcgttca tgcgtgaccc gcgattccgt
      181 ggtttcacgg gggatgatac ggaggtacag gcccgccttg cccgtggcaa cgcgccgatg
      241 gcggaggagc ttgtacgttc acagatggct gagggtgatg ccggtccgac agcacaggag
      301 ttaactgagc gtccgcgtct gcccgccccc ggcgatattc atcccggaca gggatatccg
      361 ttaccgggag aggtggcgcg tacgccggat gagaatcagg ccggacgtgg tggtcgtttt
      421 accacaaccg gtgaggttaa gggccagagt ttccagaaag gacgtgctcc ggcaccggaa
      481 aacgccgctg gtcgccaggg ggaaacactc gagggtgaaa tagttcgtcg tggtctgccg
      541 tcaccggatg cgcagaacgc gacagcaccg gtacgtgaag ggctaccggc tcctgacatt
      601 gcgcgtaatg ttcgtatgcc tcagcctgaa tcacttcccc gcactgtacg ggactcactg
      661 cctgagctcg cacagcaggc agaagtacgc cgacaggccg gaggaaatcg tgacatcccg
      721 cagcctgaga caatcgcacc tgaatctgaa acaactgtct ttactgacag ggaatctacc
      781 gtgcgcggag gtgaagtcag gggcaaaaaa attgaagact ttggcgagga aattaaaggg
      841 gcagccaaac accgttatgc acagcttgct gaaacactgg gtaaaacgct ggaagacagg
      901 gattatgcca cgcagccgct gagcaaactg ttcccgaaac cggactacgc aaaactggcg
      961 aacgaaggtg ccgatgctga cactctggca atgatagcgc tgtatcgtag cgatattccg
     1021 gcgaagacga aacacaatac ggcaggctgg ggggagagcg taaaaaaagt acgacacagt
     1081 gtatcggaaa tgctgaacgg aacggtcagc gcgaaacgcc tcgcagaatg gatggaaggc
     1141 agaatgccct cccgttacgc ggatacctgg caactgttac gcactctgcc accctcacag
     1201 atggacaggg cttctgctta tcgggtggta tcgggtgtgt atcaggcggc aggagggaag
     1261 cgttacgatc cgccacagaa actttattca ctgcgcaata aggacaataa ggggagtaac
     1321 ctctttttct cggaaagcag ggatgaatta ctg
//
LOCUS       DAZKHK010000362         1348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKHK010000362 DAZKHK010000000
VERSION     DAZKHK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.365"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..911)
                     /locus_tag="ACHOS5_005429"
     CDS             complement(<1..911)
                     /locus_tag="ACHOS5_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780060.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDAVGKGLKVTGKSVSDGAKASDEWLTDN
                     MSPGAVRALNTPMSEGYDDPAVWLTKGMNFVGAVAADVFAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQL"
     gene            complement(981..>1348)
                     /locus_tag="ACHOS5_005430"
     CDS             complement(981..>1348)
                     /locus_tag="ACHOS5_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780061.1"
                     /translation="ELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQPHQQKIQQQK
                     LQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRIQKARGEQARD
                     ESASSASQFIRGLKQNYAQ"
BASE COUNT          291 a          401 c          307 g          349 t
ORIGIN      
        1 agctgtgcgt gctgcgggtc ggcgtcaatg gacaaaaacg tctgctgaaa tttcggtgac
       61 tgcgccagct cagagtaatc cgcattaaga actgcatctg ctgccgccat tgcactctgc
      121 ccctgtgcac tggctgtgga atgggtgatc aggcccgcct ggaataaatc cggcattttt
      181 ttatcgacag cttctgctgc cagtgccgtg gctctttccg gctgcatccc tgccgcgatg
      241 tattttttct ccagcccggc ggtcagcatt tttcgcagtg tgacatcacc caccttttta
      301 gccacaccgc cagcaaacac atctgccgca acggcaccga caaaattcat ccccttagtc
      361 agccagactg cgggatcatc atagccttca ctcattggcg tattcagcgc acgcacagca
      421 cccggcgaca tattatcggt cagccattca tccgaggctt tagcaccatc actgactgac
      481 ttgcctgtga ccttcagacc tttaccgaca gcatcagtca ccgcgttttt gccatcaggc
      541 agggtatcga tgacctcatc agcccccctg ctgccaccgg caaaaatatc ctgcacagtt
      601 gcgacacccg gcagccccat acggctgaac tcatttaaaa tacgcgcccc tgtttttacc
      661 gggctctgaa tcatcgcatc accgagtcca cgggccattt cccctgttcc ccggacggac
      721 tgggcgaaac ctttacccat tgttggcaat acatcgccca ggctgaacga cgtactgtta
      781 tccttccagc gattcggatc agagaaaaat gcttcatagc tgtcagtttc gccgggttgc
      841 tgaatgttca ggctgttacg attctggtta ccgagttgcg cctcaggacg ctgttcctct
      901 gaataggcca tacagactcc ataaaaaaac ccggcacaat ggccgggcat caggaaatgg
      961 tgttaatgga atatcgtgaa ttactgggcg taattctgtt tcagtccacg gataaactga
     1021 gatgcagatg aagcactttc atctctggcc tgttcccccc tggccttttg aatacggaga
     1081 aaattcgcat acccgttctc cagcaatttc tgattctgag gctccagcat ttcaggctga
     1141 cgggccgcta catttctggc aaatgacagc ttatcaggat catctcccgc ccagttgata
     1201 acctgttgct gaagtttttg ttgctgaatt ttctgttgat gcggttgtga accagtcgca
     1261 gcataatatt catccaccgc agcctgtgca ttaccgccat tcttgatcgc gtccgccgct
     1321 acgttgctgg cccccttctg aagctctt
//
LOCUS       DAZKHK010000363         1346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKHK010000363 DAZKHK010000000
VERSION     DAZKHK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.366"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..612
                     /locus_tag="ACHOS5_005431"
     CDS             <1..612
                     /locus_tag="ACHOS5_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780062.1"
                     /translation="YPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTH
                     SASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQHSQTGPRTNSGSQ
                     PTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLSGTAASAGAHAHT
                     VGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            612..1193
                     /locus_tag="ACHOS5_005432"
     CDS             612..1193
                     /locus_tag="ACHOS5_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9780063.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAAFNRDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEYQKWNGTTWVKDAEAEKMFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYRVLLNRVDTSVVPDIEWPVAPIG"
BASE COUNT          384 a          303 c          350 g          309 t
ORIGIN      
        1 tacccgaaac ttgcagccgc ttatccgtca ggcgtgatcc ctgatatgcg tggctggacg
       61 attaagggca aacctgccag tggtcgggcc gtattgtctc aggaacagga cggcataaaa
      121 tcgcacaccc acagcgccag cgcatccagt acggatttgg ggacgaaaac cacatcgtcg
      181 tttgattacg gcactaaatc cacgaataac accggggcgc atacgcacag tctgagcggc
      241 tctacaagtt cagctggggc ccaccaacat agtcagactg ggcccagaac gaatagcggg
      301 agtcaaccta ctggaatgtt tccggcaggg agtactcaag ttagcgggac taatcaggtt
      361 ggtatcagtg gctcattaac cagtggcacc tcccaatggg taggtaaatc atcttcagaa
      421 ggaaaccata cacactcact gtccggcaca gcagcatctg cgggtgcaca cgcgcatact
      481 gtcggtattg gtgctcatac gcactctgtt gcgattggtt cacatggaca caccatcacc
      541 gttaacgctg ctggtaacgc ggaaaacacc gtcaaaaaca tcgcatttaa ctatattgtg
      601 aggctcgcat aatggcattc agaatgagtg aacaagcacg gaccataaaa atttataacc
      661 tgctggccgg aactaatgaa tttattggtg aaggtgatgc atacattcca cctcatacag
      721 gtctgcctgc aaacagtacc gatattgcac cgccagatat tccggctggc tttgtggctg
      781 ctttcaaccg tgatgaggca tcgtggcatc tcgttgaaga ccatcggggt aaaacggtct
      841 atgacgtggc ttccggcgac gcgttattta tttctgaact tggctcgtta ccggaaaatg
      901 tcacctggtt atccccggaa ggggaatatc agaagtggaa cggcacaacc tgggtgaaag
      961 atgcagaagc agaaaaaatg ttccggatcc gggaggcgga agaaacaaaa aacagcctga
     1021 tgcaggtagc cagtgagcat atagcgccgc ttcaggatgc tgtagatctg gagatcgcaa
     1081 cggaggaaga aaccttgttg ctggaagcct ggaaaaagta tcgggtgttg ctgaaccgtg
     1141 ttgatacatc agtagtaccg gatattgagt ggccagtagc acctataggg taaattcgta
     1201 atgattacct aaatacgtta ttcttttgtt caaaagtaat tttgctatag gtagggagta
     1261 tgttcttgga ttaagaataa gaaatcgcca tgaaagagtt tcatggcgat tacataaacg
     1321 tacaaaatat gatgaaagta gagtgg
//
LOCUS       DAZKHK010000364         1309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKHK010000364 DAZKHK010000000
VERSION     DAZKHK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.367"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1202)
                     /gene="mchF"
                     /locus_tag="ACHOS5_005433"
     CDS             complement(<1..1202)
                     /gene="mchF"
                     /locus_tag="ACHOS5_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFP9780064.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELNELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFQAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLKAAVSMLRAWSSLVMSTLINVQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTSVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMAYVLIRLVTY
                     GYYRQISEETLVKGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     IRMDLLFGG"
BASE COUNT          365 a          355 c          278 g          311 t
ORIGIN      
        1 cccccgaaga gcaaatccat cctgattaac ttaatacctg aattgatcgc atctattttc
       61 aggttaagcc agtgtgttcc ccggatcccg gccataccct ggatttttac cgtggcaata
      121 ccatacaggc tttccataaa ataggagctg gcccgggccc ccttgacaag agtttcttcc
      181 gatatttgcc ggtaatagcc gtatgtcacc agacgaataa gaacgtaagc catggtaaaa
      241 ccgagaacta tccaggtaag atatcctcca tataacagca tcatcacaaa aacacccaca
      301 accataatac tgtccatgat tgccccaacc acactggtgg taaaggtggc cctcaaagtg
      361 tcaagggagc caaaacgcga ctggatatca cctaatttac ggcgttcaaa aaacgccagc
      421 ggcagtctga gaagatggtt aaacagaccc gactgccact gtacattgat gagcgtgctc
      481 ataaccagtg aggaccatgc acgcagcata ctcaccgcgg ccttgagcaa tataaagaac
      541 ataaggccag cagaaataag cgtcagcagc cctctgtccc ccgccggaat cgcatgatcc
      601 ataaccagct gagtccccac cggcattacc agattgattg cttcaattac aactgacaga
      661 cagaaaattt tcgccagtgt tcttttaata ccgtaaatac tgttaatcag tgaacggaga
      721 cttatgcggg tctgctgggt ttccgcctgg aattcacttc cgggccagac ctccagtgca
      781 atgcccgtaa aataccggct catttcctcc cgaccgagat atcttctgcc tctggccgga
      841 tcatgcagta cataacggtt acgctttacg ctgaccagca cgacgaagtg actgaaatcc
      901 cagtggagaa tacacggcat tttgagggca ccaagctcat tcagctccag tgaaagagcc
      961 cgggtgacca tccccagctg ctccgctatt ccattgattc ctgcaaggtt tgctccacgg
     1021 gccgagagat taaacttccg gcgaagagat atcaggtcaa tattcttacc aaaatgaccg
     1081 catatcattg ccaggcaggc cagtccacat tcagcggtct ccgtctgatg aataaccgga
     1141 acacgacgtc gccagcgcat atcaagctga tttataattt gtctgaaatt cccgttagtc
     1201 attgagcggt cctgttgcac tgtgtttcat gtcatagaaa ggagaaagca tccactggta
     1261 aatacgcctt ttttccagaa atagtgtact ttcggctttc attccattt
//
LOCUS       DAZKHK010000365         1301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKHK010000365 DAZKHK010000000
VERSION     DAZKHK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.368"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1301
                     /locus_tag="ACHOS5_005434"
     CDS             <1..>1301
                     /locus_tag="ACHOS5_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9780065.1"
                     /translation="LKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQY
                     CDQTVPDGFGGTEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPS
                     DVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAI
                     LRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEI
                     CDNDYAGTLTGGRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDITAQP
                     APDRIQVSALPDGVETYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHIPEKEA
                     IVDNGARFEPLSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRL
                     TSGSGENSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYG"
BASE COUNT          261 a          383 c          431 g          226 t
ORIGIN      
        1 ctgaaaccgg catacagcaa taacccggcc tggtgcctgt gggacatgct gacccacccg
       61 cgctacggca tgggaaaacg cctgggggcc gcggatgtgg acaagtgggc actgtatgcc
      121 atcgggcagt actgcgacca gacggtcccg gatggtttcg ggggcacaga gccgcggatg
      181 actttcaatg cgtacctgtc acagcagcgt aaggtctggg atgttctggg ggatttctgc
      241 tcggcgatgc gctgtatgcc ggtatggaac ggccagacgc tgacgttcgt tcaggaccgt
      301 ccgtcggatg tggtgtggcc gtacaccaac agcgatgtgg tggtggatga taacggcgtg
      361 gggttccgct acagcttcag tgccctgaag gaccggcaca cggcggtgga ggtgaattac
      421 accgacccgc agaacggctg gcagacttcc acggaactgg tggaagaccc ggacgccatc
      481 ctgcgctacg gacgcaacct gctgaagatg gacgcgttcg gctgtaccag ccgcggtcag
      541 gcccaccgtg ccggactgtg ggtgataaag acagaactgc tggaaacgca gacggtggat
      601 ttcacgctcg ggtcacaggg gctgcggcac acgcccggtg acatcattga aatctgtgat
      661 aacgactacg ccgggaccct gaccggcgga cgcatcctgt ccatcgatgc cgccagccgt
      721 accctgacgc tggaccgtga ggtgaccctg ccggagacag ggacatcgac ggtgaacctg
      781 attaacggca gcggtaagcc ggtgcgcgtg gacatcactg cacaaccggc ccctgaccgg
      841 atacaggtca gcgccctgcc ggatggcgtg gagacatacg gtgtgtgggg gctctccctg
      901 ccgtcactgc gtcgtcgcct gttccgctgt gtttccatcc gggaaaacac ggacggcacc
      961 tttgccatca cggcagtgca gcacataccg gaaaaagaag ccatcgtgga taacggggcc
     1021 cgctttgagc cgctctccgg ttcactgaac agcgtcatcc cgccggcagt gcagcacctc
     1081 acggtggagg tgagtgcctc agacggccag tatctggcgc tggcgaaatg ggacacgccg
     1141 cgggtggtga agggcgtgcg cttcagtctg cgcctgacca gtggcagtgg tgaaaacagc
     1201 cgcctggtga ccaccgccat cactgccgac acggagcacc gtttcagtgg cctgcctctg
     1261 ggggaataca ccctgacggt cagggcgata aacagctacg g
//
LOCUS       DAZKHK010000366         1297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKHK010000366 DAZKHK010000000
VERSION     DAZKHK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.369"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..61)
                     /locus_tag="ACHOS5_005435"
     CDS             complement(<1..61)
                     /locus_tag="ACHOS5_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780066.1"
                     /translation="MAIKGLEQAVENLSRISKTA"
     gene            complement(73..426)
                     /locus_tag="ACHOS5_005436"
     CDS             complement(73..426)
                     /locus_tag="ACHOS5_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9780067.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(438..833)
                     /locus_tag="ACHOS5_005437"
     CDS             complement(438..833)
                     /locus_tag="ACHOS5_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9780068.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            complement(875..>1297)
                     /locus_tag="ACHOS5_005438"
     CDS             complement(875..>1297)
                     /locus_tag="ACHOS5_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9780069.1"
                     /translation="PKGWALFRSFKAVKEKLDTRRGSHSELETAVKDLGEAVSYKGMY
                     GDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGLRTYGCIQDADAQREGINASAR
                     YPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQLA"
BASE COUNT          267 a          419 c          334 g          277 t
ORIGIN      
        1 ccgccgtttt gctgatacgg ctgaggtttt caacggcctg ctcaagacct tttatggcca
       61 tacatccccc tttcagcggc gacggttaac ggcaggcggc acgccacgcc caagccagag
      121 atgacagctt ccgccatcat ccggcgaaat ccggtccacc cagaagtttt cctcgccgat
      181 ggtcagtgtg tctccacgcc gcagctgccg cacatcatca gtccggacaa acagggacgg
      241 gctggagcct tcaacgcgca ccccctgtcc ggcatagctg atattttcag ggtcatcaaa
      301 aacaccacgt atcacagcac cggacagctc accggatgtc atggtggctg acgttcccat
      361 gtacccgcgt atcgtttcat cggcgcgggc aatggcagca tcgaacaggt tatcggaatc
      421 agccacagcg cctcccgtta ttgcattctg gccaggccat gttctgtcat ttcggctgcc
      481 acaccggcag agacacgaaa cgccgttccc ggcagcacaa atgccacagg ctcatcccgc
      541 gtggcgtgaa gtgcatcagt atgcagcgtc accagtgcca cgaccgtgac cagagcagcc
      601 gtatcaatca cggtatccgg ctgcgctgat accacctcat tttcatgtcc ggtcagcgca
      661 ttttccgggc tgacagacgt gtcctgaccg gcagcgtcat ccgtgtcatc aagctcctct
      721 tccagctctg ccacacggag caccagttct tctttcgtcc ccgtcaggct gacatcacgg
      781 ttcagttgct cacccagcac ctgaagacgg gcaatcagtt catctttcgt catggactcc
      841 tccacagaga gaaaatggcc ccgaagggcc atgattacgc cagttgtacg gacacgaacg
      901 catcaggatc agccagcagc atcagcggtg ctgactgaat catggtgaac tcacgcgccg
      961 gatcgccggt ggtcacccag tttttcgggt agcgggcaga ggcgttaata ccttcgcgct
     1021 gtgcgtccgc atcctgaatg cagccatagg tgcgcagacc gcgtgcctga gtgttcccca
     1081 gcaccatcgt gttgtccggc aggaagttct ttttgacgtc gttttccacg tactgtccgg
     1141 aatacacgac gatcgccgta tcgccataca tccccttata ggacaccgct tcgcccaggt
     1201 cttttaccgc tgtctccagc tcggaatgag agccgcgacg ggtatccagc ttctccttga
     1261 cggctttgaa ggaacggaac agcgcccagc ctttcgg
//
LOCUS       DAZKHK010000367         1293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKHK010000367 DAZKHK010000000
VERSION     DAZKHK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.370"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..1280
                     /locus_tag="ACHOS5_005439"
     CDS             72..1280
                     /locus_tag="ACHOS5_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1414 family transposase"
                     /protein_id="HFP9780070.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKLSVEAALNAEMT
                     HHLGYEKNQSRPGANSRNGYSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYAKGMTTREIATAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRD
                     LKAIYQAPTEEAGQQALEAFAAAWDSRYPQISRSWQANWPNLATFFAYPTDIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQSASQKWTMSLKDWRMAMSRFII
                     EFGDRLDGHF"
BASE COUNT          355 a          319 c          333 g          286 t
ORIGIN      
        1 agcctgtaca tagatttgtg taattgcctg attttgatat gttcaatcca gcatcaaatg
       61 aaggttaatt tatggacgaa aaacagttac aggctctggc taacgaactg gctaaaaacc
      121 tcaaaacccc tgaagacctc agtcagtttg atcggctgct gaaaaagctc agcgttgaag
      181 ccgctctcaa tgcagagatg acacaccatc ttgggtatga gaaaaatcag tccaggccag
      241 gagctaactc ccgcaacggt tattccacaa agaccgttat cacaggcgac ggtccactgg
      301 aactgcgtac tccgcgcgat cgtgacggta ccttcgaacc acaactggta aagaaaaatc
      361 agacccgtat taccgggatg gataaccaga tcctctcgtt gtatgccaaa gggatgacca
      421 cccgtgagat agctactgcg ttcaaagaac tgtatgacgc agatgtttca ccggcactga
      481 tatcaaaagt caccgatgcc gtgatggagc aggttgtaga atggcaaaac agaccactgg
      541 atgctgttta ccccattgtt tatcttgact gtatcgtcct gaaagttcgg caggacagtc
      601 gcgtcatcaa caaatcggtg ttcctggcac tgggcatcaa tatcgaaggt cagaaagaac
      661 tgctgggtat gtggctggcc gaaaatgaag gggcgaagtt ctggctcaat gtgctgactg
      721 aactgaaaaa ccgcggtctg aacgatatcc tcatcgcctg tgtggatggc ctgaaaggct
      781 tcccggatgc catcaacaca gtatatccga aggcccgcat ccagttatgc atcgtgcata
      841 tggtgcgcaa cagcctgcgc ttcgtgtcat ggaaggacta caaagccgtc actcgcgacc
      901 tgaaagcgat ttatcaggct cccacggaag aggcaggtca gcaggcactg gaagcgttcg
      961 ctgcggcctg ggacagtcgc tatcctcaga taagccgaag ctggcaggct aactggccga
     1021 atcttgccac gttcttcgct tatccaacgg acatccgcaa agtgatctat acgacgaatg
     1081 ccatcgagtc gctaaacagc gtgatccgcc atgcgatcaa aaagcgtaaa gtgttcccga
     1141 cagacgactc ggtgaaaaaa gtggtgtggc tggcaatcca gtctgcgtcc cagaaatgga
     1201 cgatgtcgtt gaaggactgg cgaatggcaa tgagccgctt tattatcgag ttcggtgacc
     1261 gcctggacgg tcacttctga gaaaaggcat tta
//
LOCUS       DAZKHK010000368         1292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKHK010000368 DAZKHK010000000
VERSION     DAZKHK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.371"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1292
                     /locus_tag="ACHOS5_005440"
     CDS             <1..>1292
                     /locus_tag="ACHOS5_005440"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP9780071.1"
                     /translation="DFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELY
                     LDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWH
                     APAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWH
                     KFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHD
                     ALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLG
                     CGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTK
                     VQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERL
                     RLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELL"
BASE COUNT          287 a          342 c          358 g          305 t
ORIGIN      
        1 ggatttccgc tcagaactac tcgattcacg ttttggcgca aaggccattt ctactatcgc
       61 ggagtcaaaa cgatttccgc tgcacgaaat gcgcgatgat gttgcatttc agattatcaa
      121 tgatgaatta tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg
      181 ggacgacgaa aacgtccata aattgatgga tttgtcgatc aataaaaact ggatcgacaa
      241 agaagaatat ccgcaatccg ctgcaatcga cctgcgttgc gtaaacatgg ttgccgatct
      301 gtggcatgcg cctgcaccga aaaatggtca ggccgttggc accaacacca ttggttcttc
      361 cgaggcctgt atgctcggcg ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc
      421 cgcaggcaaa ccaacggata aaccaaacct ggtgtgcggt ccggtacaaa tctgctggca
      481 taaattcgcc cgctactggg atgtggagtt gcgtgagatc cctatgcgcc ccggtcagtt
      541 gtttatggac ccgaaacgca tgattgaagc ctgcgacgaa aataccatcg gcgtggtgcc
      601 gactttcggc gtgacctaca ccggtaacta tgagttcccg cagccgctgc acgatgcgct
      661 ggataaattc caggccgaca ccggtatcga catcgacatg cacatcgacg ccgccagcgg
      721 tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt
      781 gaaatcgatc agtgcttcag gccataaatt cggtctggct ccgctaggct gcggctgggt
      841 tatctggcgt gatgaagaag cgctgccgca ggaactggtg ttcaacgttg actatctcgg
      901 tggtcagatt gggactttcg ccatcaactt ctcccgcccg gcaggtcagg tgattgcaca
      961 gtactatgaa ttcctgcgcc tcggtcgtga aggctataca aaagtacaga acgcttccta
     1021 ccaggttgct gcttatctgg cggatgaaat cgccaaactg gggccgtatg agttcatctg
     1081 tacgggtcgc ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga
     1141 tccgggatac accctgtatg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc
     1201 ggctttcact ctcggcggtg aagccactga catcgtggtg atgcgcatta tgtgtcgtcg
     1261 cggcttcgaa atggactttg ctgaactgtt gc
//
LOCUS       DAZKHK010000369         1282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKHK010000369 DAZKHK010000000
VERSION     DAZKHK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.372"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(16..189)
                     /gene="gnsB"
                     /locus_tag="ACHOS5_005441"
     CDS             complement(16..189)
                     /gene="gnsB"
                     /locus_tag="ACHOS5_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9780072.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDIKIDLI"
     gene            complement(362..592)
                     /gene="ynfN"
                     /locus_tag="ACHOS5_005442"
     CDS             complement(362..592)
                     /gene="ynfN"
                     /locus_tag="ACHOS5_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFP9780073.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
     gene            complement(624..662)
                     /locus_tag="ACHOS5_005443"
                     /pseudo
     CDS             complement(624..662)
                     /locus_tag="ACHOS5_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(665..853)
                     /gene="ynfQ"
                     /locus_tag="ACHOS5_005444"
     CDS             complement(665..853)
                     /gene="ynfQ"
                     /locus_tag="ACHOS5_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9780074.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSRLSLDLRK"
     gene            complement(864..1076)
                     /gene="cspI"
                     /locus_tag="ACHOS5_005445"
     CDS             complement(864..1076)
                     /gene="cspI"
                     /locus_tag="ACHOS5_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFP9780075.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSVIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          425 a          250 c          203 g          404 t
ORIGIN      
        1 aatactataa acatactaga ttaaatcaat cttgatatca tagctttcaa gaccagtcat
       61 tttttcccgt gcagtaaact ggatactggt aacttctttc ccggtctttt tcttaagctc
      121 aatgattttt tttgttatat attcagaaat atctgcttct gtttttgttt ttaactcttc
      181 aatgttcatc atttcctctt ttagtctgtt atgactttcc tggtacacag tagtgtcaat
      241 tatatggagc aaacgtataa aagataagat gaaacatcgc aataatcaac atacgacggt
      301 ctaaatttca cacaaacaga taaagatgat tatcatttat tatcaatgca ttagaatcaa
      361 atcaattcaa gagtctcatt gctgcttcca gaatttcttc tgaagttaca tgtcgatccg
      421 ctgctacata aatgacttta tgatctccgg tcagagatgg aaaccctgca gccattacag
      481 tgaggtgtgt tgtttcgcca tttggatatt cacgcatgat ggtgttaact ccggtcatca
      541 ctggcactac cattgctggt tcagagttaa aaaaaactat tatttttttc ataatgttac
      601 cgtaatatgt gagtatccat cgactagaca ctaagcaaaa aagctcccga aggagccttc
      661 attttcactt tcttaaatct aacgacagac ggctaacatt taagtattgt gaaatattat
      721 caaatgtaat catcattgat ttacaaaaga tacattttgc cccgaaagga ttcctgtcag
      781 aaacatcaaa agatgatgtt ctatactgag aaccatggca acacgggcat ctaaagtgaa
      841 tatggtttgt aatattttct acctcaaagt gccactacat gaacagccgc ggggccttta
      901 ggtccgttct caataccaaa ttcgacttcc tgattctcag ttaacgtttt gaaatcgttg
      961 ctctgaataa ctgagaaatg gacaaacaca tctttgctac catctttcgg cgtgatgaaa
     1021 ccaaaacctt tttcagggtt aaaccatttc actaaaccag tcattttgtt agacatcatt
     1081 attacctttt gaagaagtta gcccttgggc agaatggtcc gaaaaaaaat atcagagaga
     1141 aaaaccaaca aggagatctc aagaggtaca aataataaaa ttataacaat gactgcttca
     1201 gataattttg taacaaacca gaacaccatt aacgcatgat taaccaacca tagcaaggat
     1261 tacttttgta aagaaaaaca ca
//
LOCUS       DAZKHK010000370         1270 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKHK010000370 DAZKHK010000000
VERSION     DAZKHK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.373"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(441..1073)
                     /locus_tag="ACHOS5_005446"
     CDS             complement(441..1073)
                     /locus_tag="ACHOS5_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP9780076.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          346 a          267 c          253 g          404 t
ORIGIN      
        1 atctgcaaaa tatcgtcgta gctgcactcg atatttttaa attcctgtat caagtgctga
       61 aaatcatttc atcgctactt ccaattcctt tctgcatggt aagtagtgag gacgaaaaga
      121 agaatataat aaatattggt tcgcggtaat cagagagagc cgcgacattt gtgatgttat
      181 tcttacaact gtaagaagtg tcagtgattt acaagtaagg ttatgattac actatttaaa
      241 gcgtaccata aaatataaca taataaatga tttgtgataa tatgggatta ttgcggttta
      301 ctttgaggaa gataaaatgc gagtttatag aaccgcgagc gccaaataat tttatgacca
      361 caacctgtcg tattgtggtc ataaaaaatt gtcggctgga gggagtaaaa tcctttacct
      421 caatatattg cctgtcaggt ttagttcaca aacagtgctt cgctatcaac gatgattcca
      481 tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt gctttttatc aagcagtttc
      541 tgcgcatcag caagtgcatt acgagtctgt ttaagcggca tcaaatattg gttttccatt
      601 acaccaacac cggccaggcg aagctcttcc attgcaccct ttttatcccc tttcgccatt
      661 ttttcgttgg caatttttat cgcggcttct ttttcggggg ttgctacata actctcagaa
      721 attccgacag atgcattaat gacaatatat tggtcatcat tgacgttggt ctttttacct
      781 ggcttagcga actttgccca ttcggtacta tcatctgaca gcaaagcgga agcttcattg
      841 gtcagttctt ttgccttttc cggatcgcca tgaaacagag ccaggcgcgc tacctgcacg
      901 tcacgcatcg catataaacc ttgttcagag atacgctctg ctgctgccgt tttctgggtt
      961 tgcttaactt gctgagtttg ctgttgtgcc gctaccggtg cattatcagc agcccataca
     1021 gttgaagatg ccagaattgc tgtgaccatc gtagccataa ttaaacgttt catacttacc
     1081 tcattcttta acagttttag aaaacgccgc catcatattg ggtgagcatg aaatgagaaa
     1141 tcagagagca acggacattt tgaaagggtt atcttacaac tgtagttatt atcgcaaagc
     1201 cgcttttagt cttacagttg taggaatctg cttcctgtcg cagagaacta ttcttatata
     1261 ccacaaagat
//
LOCUS       DAZKHK010000371         1263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKHK010000371 DAZKHK010000000
VERSION     DAZKHK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.374"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1263
                     /locus_tag="ACHOS5_005447"
     CDS             <1..>1263
                     /locus_tag="ACHOS5_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9780077.1"
                     /translation="QYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARP
                     EALRRFELMVEEVARNASAVAQNTAAVKKSASDASTSAREAATHATDAAGSARAASTS
                     AGQAASSAQSASSSAGTASTKASEASKSAAAAESSKSAAATSAAAAKTSETNAAASQQ
                     SAATSASAATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAANSAKAA
                     KTSETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAAS
                     SASTATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSA
                     SSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKR
                     AEDIASAVALEDASTTKKGIVQLSSATNS"
BASE COUNT          322 a          383 c          390 g          168 t
ORIGIN      
        1 cagtacagcg ttattctgtt ggtggaggga ttcccgccgt cacatgccgg gaccatcacc
       61 gtgtatgaag attcccgacc cggtacgctg aatgattttc tcggtgccat gacggaggat
      121 gatgcccgtc ctgaggcact gcgccgtttt gaactgatgg tggaagaggt ggcgcgtaac
      181 gcgtccgcgg tggcacagaa cacggcagcc gtgaagaagt cagccagcga tgccagcaca
      241 tcagcccgtg aggcggcaac ccatgcgact gatgctgcag gctcagcacg cgcagccagc
      301 acgtcagccg gacaggccgc gtcgtcggct cagtcagcat cttccagcgc aggaacggca
      361 tcaacaaagg ccagtgaagc atcgaaaagt gctgctgctg cagagtcctc aaaaagcgcg
      421 gcagctacca gtgccgctgc ggcgaaaacg tcagaaacga atgcggcagc gtcacaacaa
      481 tcagcagcca cttctgcatc cgccgcgacc acgaaggcgt cagaagcagc cacctcagcc
      541 cgggatgcgg cggcctcaaa agaggcagcg aaatcatcag aaacgaacgc atcatcaagc
      601 gccagtagtg ccgcttcctc ggcaacggcg gcagcaaatt ctgcgaaggc ggccaaaacg
      661 tctgagacaa acgccaggtc ttctgaaacg gcagcgggac agagcgcctc ggctgcggca
      721 ggctcaaaaa cagcggctgc gtcgtctgcc agtgccgcgt caacaagtgc cgggcaggcc
      781 tcagccagtg ccaccgccgc cggaaaatcg gcagaaagtg ccgcatcgtc tgcttcaaca
      841 gccacaacga aggctggcga agccactgaa caggccagcg cagcagcgag gtctgcttcc
      901 gcagcgaaga catccgagac gaacgcgaaa gcgtcggaaa ccagcgcaga atcctcaaaa
      961 acggctgccg catcgtccgc cagttcggcg gcgtcatcgg catcatcggc gtctgcttca
     1021 aaagatgagg cgaccagaca agcgtccgca gcgaagggca gcgccacgac agcatccacg
     1081 aaggcgacag aggcagctgg cagtgcgacg gcggcagcac agagcaaaag tacggcggaa
     1141 tccgcggcaa cgcgcgccga gacagcggca aaacgggcag aggatattgc atccgccgtg
     1201 gcgcttgagg atgcaagtac gacgaaaaag gggatagtac agctcagcag tgcgaccaac
     1261 agt
//
LOCUS       DAZKHK010000372         1188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKHK010000372 DAZKHK010000000
VERSION     DAZKHK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.375"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..147
                     /locus_tag="ACHOS5_005448"
     CDS             <1..147
                     /locus_tag="ACHOS5_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9780078.1"
                     /translation="DWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQ
                     KGAA"
     gene            144..>1188
                     /locus_tag="ACHOS5_005449"
     CDS             144..>1188
                     /locus_tag="ACHOS5_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9780079.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLLPAMNLCQLPG
                     RAGALLTTLLHHISTINRFDNLMTQPLLSDGPLTVLMDHYLDTDDLADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGTGTTKNSVFQHIQSLPHGQQKEALLASAALPLLFRPREVQG
                     KMYGDGGMGGWQNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRRAFPDTTILEIRPR
                     KKLKQTGEEGKSGGLLSFTSAHIDTWRQQGYEDTMLAMEHIRKPLEARQALTRSEAVL
                     QKSLEKTEEADLAL"
BASE COUNT          281 a          288 c          357 g          262 t
ORIGIN      
        1 gactggtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc tgcatgagta cagaaatgaa aacggggctg gtgctgtccg
      181 gtggcggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtggta
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcgg
      301 cctctcccga tctgtcagaa gctgccgtac gcctggaggc gctctgggat catctgggga
      361 ataatcaggt gctgtcggta aacagattgg tttacttttc attgctgaaa aaactgctcc
      421 cggccatgaa cctctgccag ctccccggac gtgcgggcgc actgcttacg acgcttcttc
      481 accatatatc gacaatcaac aggtttgaca atctgatgac tcagccgttg ttgtcagatg
      541 ggcccctgac agtgctgatg gatcattatc ttgatactga tgatctggct gacgggctac
      601 cgttgtatgt gtcgctgtac cccacggaag ggggaatgca ggatattatc gactgcattc
      661 gtgctgaact gggtaccgga actacaaaaa actccgtttt tcagcatatc cagagcctgc
      721 cccacgggca gcagaaagaa gccttactgg cgtcagcagc actgcccctg ctgttccgtc
      781 cccgtgaggt acagggaaaa atgtacggtg atggtggtat gggcggatgg caaaatatgc
      841 aggggaatac ccctgtgacg ccactggtgg atgccggatg caatatggtg attgtgtcgc
      901 atctgagtga cggttcttta tgggatcgcc gggcttttcc ggacaccaca atccttgaaa
      961 tccgtccccg gaaaaagctg aaacaaacag gggaagaagg caaaagtggc ggtctgctca
     1021 gttttacatc ggcacatatc gacacctggc gtcagcaggg ctatgaagac acgatgctgg
     1081 cgatggagca tatccggaaa ccactggagg cgcgtcaggc actgacccgg tcagaggcgg
     1141 tgttgcagaa aagtctggag aaaacggaag aggcagattt ggcactga
//
LOCUS       DAZKHK010000373         1183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKHK010000373 DAZKHK010000000
VERSION     DAZKHK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.376"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1183
                     /locus_tag="ACHOS5_005450"
     CDS             <1..>1183
                     /locus_tag="ACHOS5_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780080.1"
                     /translation="RFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQEL
                     TERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAP
                     ENAAGRQGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVR
                     DSLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFG
                     EEIKGAAKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIA
                     LYRSDIPAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQ
                     LLRTLPPSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDE
                     LL"
BASE COUNT          308 a          303 c          360 g          212 t
ORIGIN      
        1 acgattccgt ggtttcacgg gggatgatac ggaggtacag gcccgccttg cccgtggcaa
       61 cgtgccgacg gcagaggagc ttgtacgttc acagatggct gaaggtgatg ccggtccgac
      121 agcacaggag ttaactgagc gtccacgcct gcccgctccc ggcgatattc atcccggaca
      181 gggatatccg ttaccgggag aggtggcgcg tacgccggat gagaatcagg caggacgtgg
      241 tggtcgtttt accacaaccg gtgaggttaa gggccagagt ttccagaaag gacaagctcc
      301 ggcaccggaa aacgccgctg gtcgccaggg ggaaacactc gagggtgaaa tggttcgtcg
      361 tggtctgccg tcaccggatg cgcagaacgc gacagcaccg gtacgtgaag ggctaccggc
      421 tcctgacatt gcgcgtaatg ttcgtatgcc tcagcctgaa tcacttcccc gcactgtacg
      481 ggactcactg cctgagcttg cacagcaggc agaagtacgc cgacaggccg gaggaaatcg
      541 tgacatcccg cagcctgaga caatcgcacc tgaatctgaa acaactgtct ctactgacag
      601 ggaagctacc gtgcgcggag gtgaagtcag gggcaaaaaa attgaagact ttggcgagga
      661 aattaaaggg gcagccaaac accgttatgc acagcttgct gaaacactgg gtaaaacgct
      721 ggaagacagg gattatgcca cgcagccgct gagcaaactg ttcccgaaac cggactacgc
      781 aaaactggcg aacgaaggtg ccgatgctga taccctggca atgatagcgc tgtatcgtag
      841 cgatattccg gcgaagacga aacacaatac ggcaggctgg ggggagagca taaaaaaagt
      901 acgacacagt gtatcggaaa tgctgaacgg aacggtcagc gcgaaacgcc tcgcagaatg
      961 gatggaaggc agaatgccct cccgttacgc ggacacctgg caactgttac gcactctgcc
     1021 accctcacag atggacaggg cttctgctta tcgggtggta tcgggtgtgt atcaggcggc
     1081 aggagggaag cgttacgatc cgccacagaa actttattca ctgcgcaata aggacaataa
     1141 gggtagtaac ctctttttct cggaaagcag ggatgaatta ctg
//
LOCUS       DAZKHK010000374         1171 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKHK010000374 DAZKHK010000000
VERSION     DAZKHK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.377"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(120..338)
                     /locus_tag="ACHOS5_005451"
     CDS             complement(120..338)
                     /locus_tag="ACHOS5_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013575645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780081.1"
                     /translation="MLIYNVFGRHIGVKREGDHWLVFRADLTERKFSRLYDIVIPDDM
                     TEDEIPGWLSDIFHEAATERHPDVKRIR"
     gene            complement(569..666)
                     /locus_tag="ACHOS5_005452"
                     /pseudo
     CDS             complement(569..666)
                     /locus_tag="ACHOS5_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          292 a          258 c          295 g          326 t
ORIGIN      
        1 ctattagttt tctaaatatt gtatattttg agtattgcag gatatcccct gtaacgaagt
       61 ttgcgtaaca gcattttact ctacgagttt gccagcctcc cccagtgggc tggctttttt
      121 tatctgatgc gcttcacatc agggtgacgt tctgttgccg cttcatggaa aatgtcgctg
      181 agccagcccg gtatctcgtc ttctgtcata tcgtctggaa tgacaatatc gtataaccgg
      241 gagaattttc gttctgtcag gtctgcccgg aagaccagcc agtgatcacc ttcacgtttg
      301 acaccaatat gtcgaccaaa gacgttgtag attagcacta tcgacctctt tcaggaattg
      361 tgtaactgag atgacagatt cagatttcgt caataaatgc aaaatgtcaa tcctgagatc
      421 atggtgagct atctggcggt tttttatatc tgtttaacgg gggatgttac atacctcaca
      481 attcagtcag ttgaaggttg tctgcccgga tgagaatttg ttagaaaatt tctgcatggt
      541 gaatccccct gagcggaggg gcatatcagc gacaggtggt tggttatatc ccttatctgg
      601 tgcgggttca ggtgctgata ctgaactcac cgggaggcac ccggcaccat gcaatgaacg
      661 gtacatagcg ctactcttca gcccctctcc ggaggggctt tttaatgagg ggaagacact
      721 gggcagtgaa atgttaaatc actcacaatt caggtagttg acggttgtct gcttggctga
      781 gaatttgtaa aaaaaatcct gcatggtgaa tccccctgtg cggaggggcg actggtgaat
      841 ggtatgatct ctttgatgat cgtaagcgag aatacgcggg tttggtggca ccaggccgaa
      901 ctcaccggga ggcacccggc accatgcagt atacagagat taggcatata ccaaggcccc
      961 tcatagcagg ggccttttta catgcaaaaa aagccgcccc gggaagagcg gttggcaaga
     1021 agaaaagcaa tatgaacaat taattaacga tgggaataat accttagagt aatcaccttg
     1081 cgcaactgta agggcatatt tcatctttgc gggctgtttt tccgggtgac ttctgtgttt
     1141 ccggaggcca gcctgtacct tctctgactc a
//
LOCUS       DAZKHK010000375         1162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKHK010000375 DAZKHK010000000
VERSION     DAZKHK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.378"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1062
                     /locus_tag="ACHOS5_005453"
     CDS             <1..1062
                     /locus_tag="ACHOS5_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9780082.1"
                     /translation="SNDAEGYLGLFREKIGKLHLAQGMWELIDNSQLDDEMAEMKTTI
                     TETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIG
                     AGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSIT
                     SSGNPPTFSLTPEGRLTARNADISGAVTANSGTLNNVTINENCVIRGKLSANQIEGDL
                     VKTVGKAFPRNNSYASGTITVTVYDDQGFNRQIIIPPVLFRGARHEHQSSKDHTSTWY
                     STCQLQVQMNGRVIFHQPATDVTRVFSSVIDMPAGRGHVTLTFNVWSNGVNHWTPTTY
                     ISDLLVVVMKKSTAGIVIS"
BASE COUNT          309 a          282 c          345 g          226 t
ORIGIN      
        1 agcaatgatg cagaagggta tctggggctg tttcgggaaa agataggaaa actgcatctg
       61 gcacagggga tgtgggagct gattgataac agccagcttg atgatgagat ggcggagatg
      121 aagaccacca tcaccgaaac ccgcaatgaa atcacgcaaa cggtcagtaa aaccctggaa
      181 gaccagagcg ccaccataca gcagatacag cgggtgcaga cagacacaaa taacgacctg
      241 gctgcgctgt acatgctgaa ggtgcagaaa acgaaaaacg gcattccgta tgttgccggt
      301 attggtgcgg ggattgagga tgctgatggc cagcccctga gcaatatact gctgcaggcg
      361 gaccgtatcg cgatgattaa cccggagaac ggcaacacca cgccgctgtt tgtggcgcag
      421 gggaatcagc tgttcatgaa cgatgtgttc ctgaagcgac tgtttgcagt gagcatcacc
      481 tcgtccggca atcccccgac gttctccctg acgccggagg gcaggctgac ggcccgcaat
      541 gcggacatca gtggagctgt cacagcgaat agcggcacgc tcaataatgt caccattaac
      601 gagaactgtg tcatcagagg gaaactgtct gcgaaccaga ttgaaggcga tctggtgaag
      661 acggtgggga aagcctttcc ccggaataac agttatgcca gtggcacgat aaccgtcacg
      721 gtttacgatg accagggctt caaccggcag attatcattc caccggtgct gttccggggg
      781 gcgagacatg agcaccagtc cagcaaagat cacacttcaa catggtattc cacgtgtcag
      841 ttacaggtgc agatgaacgg aagggtaatt tttcatcagc cggcaacgga tgtcacccgg
      901 gtgttttcgt ccgtcattga tatgccagca ggacggggtc atgtgaccct gacgtttaat
      961 gtgtggtcga acggggtgaa ccactggacg ccgacaacat acatcagtga tttactggtt
     1021 gtggtcatga aaaaatccac ggcggggatc gtcatcagtt gaacatcgcg ccccgggatt
     1081 gccggaagga gacaaaaacc gtacattatg cgcgggtgcc tttggctgat ggccggaggg
     1141 aacacctgaa ggccggaaag ac
//
LOCUS       DAZKHK010000376         1160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKHK010000376 DAZKHK010000000
VERSION     DAZKHK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.379"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            44..>1160
                     /locus_tag="ACHOS5_005454"
     CDS             join(44..293,293..>1160)
                     /locus_tag="ACHOS5_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFP9780083.1"
                     /translation="MSRKIQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISP"
BASE COUNT          334 a          277 c          296 g          253 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaatcca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa
      301 agcaacagcg tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga
      361 caacaatttc ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac
      421 aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag
      481 attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag
      541 ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta
      601 aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa ccacaatctg
      661 ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg
      721 gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa
      781 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
      841 acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac
      901 cactccgatc gtggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt
      961 ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc
     1021 ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa
     1081 gccatctcag taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct
     1141 cgtctgggga atatctcccc
//
LOCUS       DAZKHK010000377         1159 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKHK010000377 DAZKHK010000000
VERSION     DAZKHK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.380"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..487
                     /locus_tag="ACHOS5_005455"
     CDS             32..487
                     /locus_tag="ACHOS5_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFP9780084.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            566..799
                     /locus_tag="ACHOS5_005456"
     CDS             566..799
                     /locus_tag="ACHOS5_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP9780085.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            899..>1159
                     /locus_tag="ACHOS5_005457"
     CDS             899..>1159
                     /locus_tag="ACHOS5_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9780086.1"
                     /translation="MRLASRFGYANQIRRDHPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLR"
BASE COUNT          305 a          308 c          281 g          265 t
ORIGIN      
        1 gtcatcgtca tgactcagag aggtaaatac catgattcac ctgttcaaaa cctgcatgat
       61 taccgccttc attctggggt taacgtggtc tgccccactc cgggcacagg atcaacgtta
      121 catcagtata cgcaatacag atacgatatg gctcccggga aatatttgtg cttaccagtt
      181 ccggctggat aatggcggaa acgatgaagg atttggcccc ctcaccatca ctctgcaact
      241 caaagacaaa tatggtcaga cgctggtgac cagaaaaatg gaaacggaag cttttggtga
      301 cagtaatgcc acgcgaacca cagacgcatt tctggaaacg gagtgcgtgg aaaatgtcgc
      361 cacaaccgaa atcattaaag caactgaaga aagtaacggc catcgtgtca gtctgccgtt
      421 atcggttttc aatccccagg actaccatcc actgctgatt accgtttccg gaaaaaacgt
      481 taactgatcc ctgaagtcag tttcatgctt cagcctctgt cacaacgcgt ggcagaggct
      541 ttttatttat caggagaaaa cagatatgcc gggttacaca gagtatgtcc tggctgaagg
      601 atctttctca tacgggcagg cggttgctgt cataaccgct taccgcaatg tgtttatcca
      661 ggatgacccg ggaatgcatt tccgtcgggt tatccgcaac gcggaaggtc agcgacgctg
      721 gcgctgccgg aattctgaac ccgatgccgg aaaggtgctt aatacgcggc tcgccagtga
      781 cggtcttctc agacaataaa cgtcattcat ttcacccatc aggccgcgtc ttctccggga
      841 gacgtggctt tttcatttat tacaccagta aatcttaacc accgataagg agcaaattat
      901 gcgactagcc agtcgttttg gttatgcgaa ccagatacgc cgtgaccatc cgctgacaca
      961 cgaagaactg atgcactatg tgccggggat tttcggggaa gataaacaca cgtcccgaag
     1021 ccagaactat acgtacatcc ccaccatcac cgtactggaa agcctgcagc gggaaggctt
     1081 tcagccattc ttcgcctgcc agacccgtgt gcgcgacccg ggccgccggg gatacacaaa
     1141 acacatgctg cgtctgcgg
//
LOCUS       DAZKHK010000378         1158 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKHK010000378 DAZKHK010000000
VERSION     DAZKHK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.381"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1059
                     /locus_tag="ACHOS5_005458"
     CDS             <1..1059
                     /locus_tag="ACHOS5_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9780087.1"
                     /translation="SNDAEGYLGLFREKIGKLHLAQGMWELIDNSQLDDEMAEMKTTI
                     TETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIG
                     AGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSIT
                     SSGNPPTFSLTPEGRLTARNADISGHISANSGTLNNVTINQNCRILGKLSANQIEGDI
                     VKTVGKAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYS
                     SCRLMVKCNGRVLFERTALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASI
                     SDLLVVVMKKSTAGISIS"
BASE COUNT          293 a          268 c          361 g          236 t
ORIGIN      
        1 agcaatgatg cagaagggta tctggggctg tttcgggaaa agataggaaa actgcatctg
       61 gcacagggga tgtgggagct gattgataac agccagcttg acgatgagat ggcggagatg
      121 aagaccacca tcaccgaaac ccgcaatgaa atcacgcaga cggtcagtaa aaccctggaa
      181 gaccagagcg ccaccataca gcagatacag cgggtgcaga cagacacaaa taacgacctg
      241 gctgcgctgt acatgctgaa ggtgcagaaa acaaaaaacg gcattccgta tgttgccggt
      301 ataggtgcgg ggattgagga tgctgatggc cagcccctga gcaatatact gctgcaggcg
      361 gaccgtatcg cgatgattaa cccggagaac ggcaacacca cgccgctgtt tgtggcgcag
      421 gggaatcagc tgttcatgaa cgatgtgttc ctgaagcggt tgtttgcagt gagcatcacc
      481 tcgtccggca atcccccgac gttctccctg acgccggagg gcaggctgac ggcccgcaat
      541 gcggacatca gcggacatat cagtgcgaac tcgggcacgc tcaataatgt gaccattaac
      601 cagaactgcc ggatacttgg aaaactgtct gccaaccaga ttgaaggcga tattgttaaa
      661 accgtgggga aggctttccc gcgggactcc cgcgcaccgg agcggtggcc atcggggacc
      721 attaccgtca ggatttatga cgatcagcct tttgaccggc aaattgtgat cccggcagtg
      781 gctttcagcg gcgctaaaca tgagagagag cataacgata tttattcatc atgccggctg
      841 atggtgaaat gtaacggcag ggtgcttttt gaaaggacgg cgctggctga gtctcaggtg
      901 tattcagggg tgattgatat gcctgcagga agggggcata tgacgctgga gttttctgtg
      961 tctgcctggc tggtgaatgg atggtacccg acagcgagta tcagcgatct gctggttgtc
     1021 gtgatgaaga agtccacggc gggtatcagt atttcgtgat catgaggagc cggattgccg
     1081 gaaggagaca aaaaccgtac attatgcgcg ggtgcctttg gctgatggcc ggagggagca
     1141 cctgaaggcc ggatgtga
//
LOCUS       DAZKHK010000379         1145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKHK010000379 DAZKHK010000000
VERSION     DAZKHK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.382"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..486
                     /locus_tag="ACHOS5_005459"
     CDS             <1..486
                     /locus_tag="ACHOS5_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9780088.1"
                     /translation="GEDTAPEAVYACLHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGV
                     AAIVGSFFTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGVAQMLAPKAKTPEYK
                     STDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGR
                     G"
BASE COUNT          313 a          228 c          346 g          258 t
ORIGIN      
        1 ggtgaggata ccgcgccgga ggcggtgtat gcctgtcttc acgaacaact gggtgaggga
       61 gcggtcatcc acattgtgcc gcgactggcc ggagccgggg gaaatggtgt ttttcaggtg
      121 gtgctggggg ttgcagcgat tgtcgggtca tttttcactg gtggtgcaac actggcggca
      181 tggggcgcag ccctgagtgc cggagggctg actgccacca cgatgctgtt ctcactgggt
      241 gccagcatga tactgggtgg tgtggcccag atgctggccc cgaaggcaaa gacgccggag
      301 tacaaaagta cggataacgg taaacagaac acgtattttt cgtcactgga caacatgatt
      361 gcccagggta acccgatgcc ggtgccttac ggtgaaatgc tggttggttc acgacggata
      421 tcccaggaca tcagcacccg tgatgagggc ggagacggga aagtggtggt tatcgggcgg
      481 ggataaaaat aaaaaaaatc ccgcagagtt agcggagctg cgggaaataa cgatgaagat
      541 taacgttatg gagttatttt tttgggcaga aaaacattaa cgcagcgaaa ttataagcgc
      601 tacatggatt ttgcgaaaat gtgaagaaat tcagaaattt tttgctgcag aggtgcaggg
      661 agcttgccgg gtatttgtgg cagaagacag aaaacatcat gccggggtta agggataaaa
      721 aaatcccgca gagtccggag actgcgggac agaaccgatg aagattaacg ttcaaggagt
      781 tttgttttta ttgacccgaa aaaactgtaa cgccccggaa tgatatctgc cacggggacg
      841 tacagaaaat gtgaagaaat tcagactttt tctgtcgcag gtttacagaa cggatgaccg
      901 ccagggtacc agtggcgtaa atgagcagga tgttgtggtg tggctgaagc aaaaaaatct
      961 cccgtaatat cgccggatac tgacgggaga cgaatagtca ccttaaggag gtctgataat
     1021 tattgctttt attgagcaaa aaaactgtaa cgctgcgtca ttatagtggc tacaggcaat
     1081 tgccggaaat gtgaagaatt tcagaaattt tattccgtca tgacacaggc accctccggg
     1141 gtgcc
//
LOCUS       DAZKHK010000380         1143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKHK010000380 DAZKHK010000000
VERSION     DAZKHK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.383"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..>1143
                     /locus_tag="ACHOS5_005460"
     CDS             14..>1143
                     /locus_tag="ACHOS5_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780089.1"
                     /translation="MLQIVGALILLITGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYN"
BASE COUNT          269 a          284 c          291 g          299 t
ORIGIN      
        1 tcacggagtg actatgttac agatagtcgg cgcgctgatc ctgctgatca caggatttgc
       61 cattcttcgc cttttgttca gagcattaat cagcacggct tctgcgctgg cagggctcat
      121 attgctgtgt ctgttcggcc cggccttact ggctggctat atcaccgaac gcataacccg
      181 gttgttccat attcgctggc tggcaggcgt atttctgacg attgccggaa tgatcatcag
      241 cttcatgtgg ggacttgatg gtaaacatat cgcgctggag gctcacacct ttgactctgt
      301 gaaatttatt ctgaccactg ctctcgccgg tggtctgctg gctgttcccc tgcagatcaa
      361 aaacattcag cagaacggga tcacaccaga agatatcagc aaggaaatta acgggtatta
      421 ctgctgtttt tatactgcct ttttccttat ggcgtgttct gcatgcgcac cattgatcgc
      481 gttacagtac gatatttcac cgtcactgat gtggtggggc gggttgttgt actggctggc
      541 tgcattagtg acgctgctat gggcggccag ccagatccag gcgctgaaaa aactgacctg
      601 tgccatcagc cagacactgg aagaacaacc ggtgctcaac agtaaatcgt ggctgaccag
      661 tttgcaaaac gattacagcc ttcctgactc actgacggag cgcatctggc tgacgctcat
      721 ttctcaacgg atttcccggg gagagctgag ggaatttgaa ctggcagacg gaaactggtt
      781 actgaacaat gcctggtatg aaagaaacat ggcagggttt aacgaacagt tgaaagagaa
      841 cctgtcattc acacctgatg aactgaaaac gctcttccgg aaccgcctga atttatcacc
      901 ggaagcgaat gacgattttc tcgatcgttg cctggacggc ggtgactggt atcccttttc
      961 agaaggtcgc cgttttgtat cattccatca cgtggatgag cttcgtatct gtgcctcctg
     1021 cgggctgaca gaagtacatc atgccccgga aaatcataag ccggatccgg aatggtactg
     1081 ctcctctctt tgtcgcgaaa cagaaacact gtgtcaggaa atttatgaac gcccttacaa
     1141 cag
//
LOCUS       DAZKHK010000381         1140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKHK010000381 DAZKHK010000000
VERSION     DAZKHK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.384"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1130)
                     /locus_tag="ACHOS5_005461"
     CDS             complement(<1..1130)
                     /locus_tag="ACHOS5_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780090.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTG"
BASE COUNT          301 a          291 c          284 g          264 t
ORIGIN      
        1 ccggtgtaag aacgttcata aatgtcctga cacagtgttt ctgtttcgcg acaaagagag
       61 gagcagtacc attccggatc cggcttatga ttttccgggg catgatgtac ttctgtcagc
      121 ccgcaggagg cacagatacg aagctcatcc acgtggtgga atgatacaaa acggcggcct
      181 tctgaaaagg ggtaccagtc accgccgtcc aggcaacgat cgagaaaatc gtcattcgct
      241 tccggtgata aattcaggcg gttccggaag agggttttca gttcatcagg ggtaaatgac
      301 aggctctctc tcagcttttc gttgaaaccc gccatgtttc tttcatacca ggcattgtcc
      361 agtagccagt ttccgtctgc cagttcaaat tccctcagtt ctccccggga aatccgttgt
      421 gaaatgagtg tcagccagat gcgctccgtc agcgtttcag gaaggctgta atcgttttgc
      481 aaactgctca gccacgattt actgttgagc accggttgtt cttccagtgt ctggcggatg
      541 gcactggtca gtcttttcag cgcctggatc tggctggccg cccatagcag cgtcactaat
      601 gcagccagcc agtacaacaa cccaccccac cacatcagtg agggtgaaat atcgaactgc
      661 aatgcgatca atggtgcgta tgcagaacac gccataagga aaaaagcagt ataaaaacag
      721 cagtaatacc cgttaatttc cttgctgata tcttcaggtg tgagcccgtt ctgctgaatg
      781 gttcttatct gcacgggaag agccagcaga ccagcggcga gagcggtggt cagaataaat
      841 tttacagagt caaaagtatg agcctccagt gcgatatgtt taccatcaag tccccacatg
      901 aagctgatga tcattccggc aatcgtcaga aatacgcctg ccagccagcg aatatggaat
      961 aaccgggtta tgcgttcagt gatatagcca gccagtaaag ccggaccgaa cagacacagc
     1021 aatatgaacc ctgccagcgc agacgctgtg ctggttaatg ctctgaacaa aaggcgaaga
     1081 atggcaaatc ctgcgatcag cagaatcagc gcgccgacta tctgtaacat agtcactccg
//
LOCUS       DAZKHK010000382         1136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKHK010000382 DAZKHK010000000
VERSION     DAZKHK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.385"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            694..1035
                     /locus_tag="ACHOS5_005462"
                     /pseudo
     CDS             694..1035
                     /locus_tag="ACHOS5_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          339 a          250 c          248 g          299 t
ORIGIN      
        1 gatttatttt tcattacaaa tgtgatatca ttcagtcaag tcacatcagg cttggagaaa
       61 ctcactgcac aatcaccagc atatttaacg tactgtgcag cttttcgggc ataaaaaacg
      121 ccctgcagcg agttcaagtt gtctggtgaa gtaatctctc ttaacaaaaa ataaactttt
      181 tatatccgta aaacgctaag agataatttt taaagttcca tcaatctcgc ggcattaccc
      241 actatccgcg taacttcagt aagatcagga cttcttcaat cgtcacctct taaccatgcg
      301 cataactgcg gatgaaagaa atcttttcgt ctcgaatcgc cagatgaata tctatattca
      361 tttttcagta agacactctt ttgcgcactg attgaaataa caatctacga gttttcgaac
      421 acttcccaca ccagagcagt ttcaaacatt ttgacatggt gagctacgct aagttcagtc
      481 taaagaatga gagagcgggt tttggagtgt atttgtgact ctcttaaaga taatcgcaaa
      541 ttttcaggcc tttcacccat aaccctgaaa aagagttatc ctctcaacaa aacgatcagt
      601 gttgcgtttg ttattttgtt gaatgcgaat attgtctcgc gcctgttcga cgattaaccg
      661 ctgaaaccac tagtgccccg tcaacctgac cagggataca gagagataaa acagacgatg
      721 caactgagca ggctgacgct gagaagtaaa aagccggagc ttgtggagca agagctgtgg
      781 ggtgtcttac tggcttataa tctggtgaga tatcagatga ttaaaatggc ggaacatctg
      841 aaaggttact ggccgaatca actgagtttc tcagaatcat gcggaatggt gatgagaatg
      901 ctgatgacat tgcagggcgc ttcaccggga cgtataccgg agctgatgcg cgatcttgca
      961 agtatgggac aacttgtgaa attaccgaca agaagggaaa gggccttccc gagagtggta
     1021 aaggagaggc tctagaaata ccccacagcc ccgaataaat atcctccggc atagccggag
     1081 gtttttcaaa tgcgcctgta aggctctctt accagccgcg ccctaacagg cgcaca
//
LOCUS       DAZKHK010000383         1136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKHK010000383 DAZKHK010000000
VERSION     DAZKHK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.386"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(42..608)
                     /locus_tag="ACHOS5_005463"
     CDS             complement(42..608)
                     /locus_tag="ACHOS5_005463"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9780091.1"
                     /translation="MYTESNVKLSSNALWQKCVLATLYRAAATHPEIKLLQFKLIPCP
                     GINKVIPSLMADFTGELHRLISEYLQCRRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(636..878)
                     /locus_tag="ACHOS5_005464"
     CDS             complement(636..878)
                     /locus_tag="ACHOS5_005464"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP9780092.1"
                     /translation="MVSTNHTRESSPYLSVPQDDFCIVNPGGCIVYFPANGRDIQDIH
                     NPDFPEQYQRLLERLDYLTKPGTKASGQRNSGYSGF"
BASE COUNT          331 a          275 c          259 g          271 t
ORIGIN      
        1 gtacaggtag caggtcagaa gtgacaatgc gtcccctgac gttaaaagtc actacaccca
       61 agatgacgtt caacagcacc atgcgattca atgtaagccc gggctgtctg ttccagtaca
      121 ccaggctcag cgttgtatgt gttagctgca tcaaatacca acgacagcac ttcaggatac
      181 acaaccagat gtgtaatgga gttatcttca cccaatactt ttccccacgc ctgctcaatc
      241 agatttctga gaaccaccac ctcacgactc ttacaccaga catcgttatt aagtagcagc
      301 accataagat aaggagtggt atcgttagcc acagcctccc tactccagag ataatataaa
      361 ggggtgggct caacagattt atctttacgt cgcctacact gcaaatattc agaaatgagt
      421 ctatgcagtt caccagtaaa atccgccatc agagagggaa tgaccttatt aataccaggg
      481 caaggtatta atttaaattg taataattta atttcaggat gtgtggctgc agcccgatac
      541 agagttgcaa ggacacactt ttgccagagg gcgttactgg aaagcttaac gtttgattct
      601 gtatacataa taaatcacct tacagttaca acaggtcaaa aaccgctgta gccagagtta
      661 cgctggcctg atgctttagt accgggcttc gtcagataat ccagacgctc caataagcgc
      721 tgatactgct cagggaaatc aggattatga atatcctgga tgtcacgtcc attagcaggg
      781 aaataaacaa tgcagccccc tggattaact atgcagaaat cgtcctgagg cactgataaa
      841 tacggagagg actctcgcgt atggttggtt gacaccacag tgcggattcg gcgaatccgc
      901 gatcacggtg cgatttcgca ccacagtaca caatcaatac cccgggtttt attcaggtaa
      961 gcaggactgc ggatatccgg tgtcacgcct ttgtgtcacg aacggggcag gtgcgaaaca
     1021 ccggataaaa tgcaggttgc gatatctctg aacgccctgc gcagagcgga gatttcggat
     1081 taagtactcg cacctccgca gtcctgaaac aagtctggct ggtagctgta aacaga
//
LOCUS       DAZKHK010000384         1132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKHK010000384 DAZKHK010000000
VERSION     DAZKHK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.387"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1110
                     /locus_tag="ACHOS5_005465"
     CDS             <1..1110
                     /locus_tag="ACHOS5_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780093.1"
                     /translation="NGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDP
                     AEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASIFWLS
                     TGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPTVRQAL
                     QKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSG
                     VAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKIL
                     AMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPL
                     VVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
BASE COUNT          256 a          290 c          329 g          257 t
ORIGIN      
        1 aacggacagt atcgttacat gggaaataat ggccccatgc aactggaagt cccccgtgat
       61 cagtatgccg gcgctgttga aaccatgagg aataagatcc gcgaaggtaa agtacccggt
      121 gtaaccgatc ccgcagaagc gtcccggctg attcgtcggg gacatctgac ttatactcag
      181 gcccgtaata tcacccggtt cgggaccatc gaatcggtca cttatgatat tgccgagggg
      241 tcggttgtca gtctggcggc cggagggatc agttttgccc tgacggcatc gatcttctgg
      301 ctcagcaccg gcgatcgcga tgctgccctg cagacagctg ctgtccaggc aggaaaaacc
      361 ttcacccgca cactggctgt ctacgtcaca acccagcaac ttcaccggct cactgttgtt
      421 cagggtatgc tgaagcatat tgatttttcg acggccagcc cgactgtccg gcaggcgctt
      481 cagaagggga ccggtgcagg aaatatcagt gccctgaaca aagtgatgaa gggtacgctg
      541 gtgacatctc tggcactggt agctgtcaca accggccctg acatgatcaa aatgttgcgg
      601 ggacggatct ccggtgcgca gttcatcagg aatcttgccg tggcatcttc cggtgtggca
      661 ggtggtgctg tcgggtcagt ggcgggcggg atattgttca gtccactggg accatttggt
      721 gcactgacag ggcgtgtggt tggcggtgtt ctggggggaa tgattgcctc cgctgtatca
      781 ggaaaaattg ccggagcgct ggttgaagaa gatcgcgtca aaattctggc aatgattcag
      841 gagcaggtga catggcttgc cggcagtttc ctgctgaccg gacatgagat tgaaaatctg
      901 aacgcgaatc tggcccgtgt tatcgatcag aatgctctgg agatcatttt cgccgccggt
      961 atacaacaac gggccgcgac caatatgtta atcaaaccac tggtggtcag tatcatcagg
     1021 caacgccccg tcatggaata tgatgcatcc catctcggca atatggttaa ccgactggaa
     1081 gaagcattac ccccggagtt accggcataa cggctattct gcgcctttta cg
//
LOCUS       DAZKHK010000385         1132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKHK010000385 DAZKHK010000000
VERSION     DAZKHK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.388"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1110
                     /locus_tag="ACHOS5_005466"
     CDS             <1..1110
                     /locus_tag="ACHOS5_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780094.1"
                     /translation="NGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVPGVTDP
                     EEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLS
                     TGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRQAL
                     QKGSGAGNISALNKVMKGSLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLAVASSC
                     VAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKIL
                     AMIQEQMTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPL
                     VVSIIRQRPVMEYDASHLGNMVNRLEETLPPELPA"
BASE COUNT          257 a          291 c          327 g          257 t
ORIGIN      
        1 aacggacagt atcgttacat gggaaatcat ggtcccatgc aactggaagt cccccgtgat
       61 cagtatgccg gcgctgtgga aaccatgaag aataagatcc gcgaaggtaa agtacccggt
      121 gtaaccgatc ccgaagaagc gtcccggctg attcgtcggg gacatctgac ttatacccag
      181 gcccgtaata tcacccggtt cgggaccatc gaatcggtca cttatgatat tgccgagggg
      241 tcggttgtca gtctggcggc cggagggatc agttttgccc tgacggcatc ggtcttctgg
      301 ctcagcaccg gcgatcgcga tgctgccctg cagacagctg ctgtccaggc aggaaaaacc
      361 ttcacccgca cactggctgt ctacgtcaca acccagcaac ttcaccggct cagtgttgtt
      421 cagggtatgc tgaagcatat tgatttttcg acggccagcc cgactgtccg gcaggcgctt
      481 cagaaggggt ccggtgcagg aaatatcagt gccctgaaca aagtgatgaa gggttcgctg
      541 gtgacatctc tggcactggt agctgtcaca accggccctg acatgatcaa aatgttgcgg
      601 ggacggatct ccggtgcgca attcatcagg aatcttgccg tggcatcttc ctgtgtggca
      661 ggtggtgctg tcgggtcagt ggcgggcggg atactgttca gtccactggg accatttggt
      721 gcactgacag ggcgtgtggt tggcggtgtt ctggggggaa tgattgcctc cgctgtatca
      781 ggaaaaattg ccggagcgct ggttgaagaa gatcgcgtca aaattctggc aatgattcag
      841 gagcagatga catggcttgc cggcagtttc ctgctgaccg gacatgagat tgaaaatctg
      901 aacgcgaatc tggcccgtgt tatcgatcag aatgctctgg agatcatttt cgccgccggt
      961 atacaacaac gggccgcgac caacatgtta atcaaaccac tggtggtcag tatcatcagg
     1021 caacgccccg tcatggaata tgatgcatcc catctcggca atatggttaa ccgactggaa
     1081 gaaacattac ccccggagtt accggcataa cggctattct gcgcctttta cg
//
LOCUS       DAZKHK010000386         1131 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKHK010000386 DAZKHK010000000
VERSION     DAZKHK010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.389"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..739
                     /locus_tag="ACHOS5_005467"
     CDS             <1..739
                     /locus_tag="ACHOS5_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9780095.1"
                     /translation="YRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARETYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQENRPEGISSLYIYSDPGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLAGGINLYQYAPNPMGWIDPLGLSG
                     EKVNVYKDAPYHGTTDNSVKSRAPING"
BASE COUNT          342 a          211 c          340 g          238 t
ORIGIN      
        1 ctaccgttat gacgaatttg gccgtgcggt ggaaaaggag gggcggagcg gaacacagag
       61 ttaccgttat gatgcggaac accggatggt ggaagtgacg acagcgcggg agacataccg
      121 gtatgtgtac gatgcgctgg ggaggcggac ggaaaaacag cacatcagcc ctgacggtaa
      181 accgtacaac cggacaaaat ttttatggga cggaatgcgg ctggcgcagg agaacaggcc
      241 ggagggaata agcagcctgt atatctacag cgacccggga agttatgaac cgctggcgcg
      301 ggtggataag gcggggaaag agggaccgaa ccggatactg tattttcaca cggatgtgaa
      361 cggtgcgccg gaagagatga cggacagcga cgggaaaatc gtgtgggaga cggggtatca
      421 ggtctggggt aacacgatac aggagaagga ccacggcggg gtggagcaga acctgcgata
      481 ccaggggcag tacctcgaca gggaaacggg gctgcactac aatctgcaca ggtactatga
      541 tccggatgtc gggcggttca tggtaacgga cccgataggg ctggcggggg gaataaatct
      601 ctatcaatat gcacctaatc caatgggatg gatcgatcca ttgggactaa gtggtgagaa
      661 ggttaatgta tataaagatg ctccttacca tggtacaact gataattcag taaaaagtcg
      721 tgcccctata aatggctaag gcgctttaga taattctgtt caagtaaaac ccacatcacc
      781 gagacgtgtt ggtgtcgacg ttgctaacaa tgaaattgtg gtattagata aaactcgctc
      841 tttgtcagat agtatggatg agtaccatgg tcatgtacgt gagtgggatg ctcttgataa
      901 taaacagcaa tcagccctga tgaaagcggg taagactacg agaaagggaa ggatttgtgg
      961 agagtaaaga taaattgcat ctggaagaat tacttaattc taaagatgaa aagcaagtag
     1021 ctgagacact cctttatata gcctacaaca ttaaggatat ttcatgggcc gaagcaatat
     1081 tcatgaagat ggctaaaaat tccaatgacg atatatgtgg gcttgcttta a
//
LOCUS       DAZKHK010000387         1101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKHK010000387 DAZKHK010000000
VERSION     DAZKHK010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.390"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1101
                     /locus_tag="ACHOS5_005468"
     CDS             <1..>1101
                     /locus_tag="ACHOS5_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780096.1"
                     /translation="CSFTEKIYLRIMLFVSSWINEGYLNEFRGDFMGVFNYKSYSFAH
                     VAELVSSSYNLAIYSNTGKVLGLGSFLVSRLFAKHPDRIDYSTDVLNEGWRDITVEEL
                     RLSSEYVDSRGFYRFPSPVTGNPAPGFGPQLKVVGLFDAQQNLQKIAISFCGTNDICD
                     IVDYTQLNNGKALSEAENLLQSIRDFAVSNGLSGEYVIVTGYSLGGGLTNIMARLSES
                     LADGFYANSDYIAWASPLVFDGDGKVLNIGFENDPVFRVLGDGAGFWEAVKAMGPGLT
                     TPDREYDSSPDNIVLYTDLYGSFLWNIIPFSVLALPLGSWSAHFAGSKTDAFERILGA
                     QFYDYMSPDSTVIVDEQSSWTSWYSWVHDKGDE"
BASE COUNT          285 a          191 c          269 g          356 t
ORIGIN      
        1 tgctctttta ctgaaaaaat ttatctgaga ataatgcttt ttgtttcttc ttggataaat
       61 gaaggatatt taaatgaatt cagaggagat tttatggggg tgtttaatta taaatcttat
      121 tcttttgcac atgtagcaga acttgtttct tcttcttata atcttgctat atattccaac
      181 actggaaagg ttcttggatt aggaagtttt ctggtatcca ggctatttgc aaagcaccca
      241 gataggattg attattctac cgatgttctt aatgaaggct ggcgtgatat tacagtggaa
      301 gaactcaggt tatcttcgga atatgtcgat tcaagaggtt tttatcgctt tcccagtccg
      361 gttacaggaa accctgcgcc aggatttggt cctcagttga aagttgttgg gttgtttgat
      421 gctcaacaga atctacagaa aattgcaata tcattctgtg gaacaaatga tatctgcgat
      481 attgttgatt atacacagct caataatgga aaagcgctgt ctgaagcaga aaacctgctt
      541 caaagtatca gagactttgc agtcagtaat ggtttaagtg gtgaatatgt tattgtcaca
      601 ggctatagcc tggggggcgg gttgactaac attatggcca gactcagtga gtctctggct
      661 gatggttttt atgctaattc tgactatata gcctgggctt ccccgctggt atttgacggg
      721 gatgggaagg tattaaatat cggttttgaa aacgatccgg tatttcgtgt gctgggagat
      781 ggagccgggt tctgggaggc agttaaagca atggggccgg gcttgacaac cccggatcgt
      841 gaatatgact cttctcctga taacattgtt ttatacacag acctttatgg ttcattcctc
      901 tggaatatta tccccttttc tgtgcttgca ctcccgctgg gaagctggag tgcgcatttt
      961 gcgggttcaa aaactgacgc ctttgaaaga attttagggg ctcagtttta cgactacatg
     1021 tcaccggaca gtacagtcat tgtggatgag caaagtagct ggacatcctg gtattcatgg
     1081 gttcatgaca agggggatga g
//
LOCUS       DAZKHK010000388         1100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKHK010000388 DAZKHK010000000
VERSION     DAZKHK010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.391"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(49..>1100)
                     /locus_tag="ACHOS5_005469"
     CDS             complement(49..>1100)
                     /locus_tag="ACHOS5_005469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780097.1"
                     /translation="DRIGTVIYTGNVNMTQGHGVTAGSGQKVKIADDDLQKDSKSFLA
                     IKAHEANVGTNVYVISKNGANNVALLEMTEGDNIITTELSLGNLGVSRWVRPMVVDTG
                     AGDDTIVTGRDLGIGYSDLWTEVNTGIGDDILIVGFGRGGYDVVYGKDGSYRIEETAK
                     YQRLDGDIIIPDNDSGYPNNGGFIEKTTVTMEDGNDSLLLLGGNSLAALKSSTLDLGA
                     GNDVIDIVANIDNASNIKGGSGFDTLVIHQGNATSNTFSGFEKIELKDGAALNLLAEN
                     LNSASIEGNKLKVTGDGSSRVDLGNNGDNLSDNGYNWIKGSTESEGDITYNIYTHASV
                     PDVQVLIDERITNVV"
BASE COUNT          326 a          284 c          167 g          323 t
ORIGIN      
        1 caacgaaaaa gatcccccca cagcaacgac tgtgagggga tactattgtt acactacatt
       61 agtgattctc tcatcaatca gaacctgtac atcaggaacc gaagcatgtg tataaatgtt
      121 ataagtgata tccccttcag actcagtgct tcctttaatc cagttatacc cattgtcact
      181 gaggttatcc ccgttattac cgaggtcaac ccgagaacta ccatcacctg tgactttgag
      241 tttattaccc tctatagagg cagaatttaa attctcagcc aacaaattca gtgcagctcc
      301 atctttcagt tcaatcttct caaaaccaga gaatgtgttg cttgtggcat taccctgatg
      361 aataaccaag gtatcaaatc cagaaccacc tttaatattt gaagcattat caatattagc
      421 cacaatatcg attacatcat taccagcacc taaatccaag gttgatgatt tgagcgcagc
      481 aagagaatta cctcctaaca ataacaggct gtcatttcca tcttccatag taacagtcgt
      541 tttctctata aaaccaccat tatttgggta tcccgaatca ttatcaggta ttatgatatc
      601 tccatccagt cgttgatact tagcggtttc ttcaatgcgg taagaaccat ccttcccata
      661 tacgacatca taaccgcctc gaccaaaacc aactatcagg atatcatcac cgatacctgt
      721 atttacctcg gtccatagat cactatatcc aatccccaaa tcacgaccag tcacgatggt
      781 atcgtcacct gcaccagtat cgacaaccat tggtcttacc cagcgagaaa cgcccagatt
      841 ccccagtgac agttcagtcg taattatatt atccccctct gtcatttcca gtagagcgac
      901 gttattagca ccattcttac ttattacata aacatttgtc cctacatttg cctcatgagc
      961 cttaatggcc aagaatgatt tagaatcttt ttgtaaatca tcatcagcga ttttgacctt
     1021 ttgacctgag ccagctgtaa ctccatgacc ttgcgtcatg tttacattac ctgtatagat
     1081 aacggttcca atccgatcta
//
LOCUS       DAZKHK010000389         1074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKHK010000389 DAZKHK010000000
VERSION     DAZKHK010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.392"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..213
                     /locus_tag="ACHOS5_005470"
     CDS             <1..213
                     /locus_tag="ACHOS5_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780098.1"
                     /translation="SILFDNNYSIRDDWENKIKDFLGDTVNSVNYKLLVSHETGPVQK
                     DLFFTRDDSGNIIYPDFFLNIINKFN"
     gene            321..>1074
                     /locus_tag="ACHOS5_005471"
     CDS             321..>1074
                     /locus_tag="ACHOS5_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780099.1"
                     /translation="MMLKKTIFILTLFSGNVIAATVELGFENEQYNYAYRSADVFMPY
                     IKSNFNPVTDSALNVSLTYMYQDQYGKKHKKTSEDRFKTNRDRIELYLKGYTLNRGAY
                     SFSPSAGFRYESWDVNYDNPKKQDKWKLELRFYPNMTYKLNDQLSLYMNGFVAPVFFK
                     TQQESRKDNNYVKGKLGAKRYNNDYYQELQILGVRYKFNNDNTLWASVYNERKYNQHS
                     SKYDRWQLRGGYDFKVTEEFVLSPFIRYDLSYR"
BASE COUNT          370 a          161 c          193 g          350 t
ORIGIN      
        1 tctatccttt ttgataataa ttattcaatc agagatgatt gggaaaataa aataaaagac
       61 ttcttaggtg atacagttaa cagtgtgaat tataaattgc ttgtttcaca tgaaacagga
      121 ccggtacaaa aagatttatt tttcacacga gatgatagtg gaaatattat ctatcctgat
      181 ttttttctta atatcattaa taaatttaat tagacaatgc ccattaacct gctgcctacc
      241 ggaagcaggt tattccagaa tggacactgg cagtaatata tctttgttga caaaaatata
      301 aaattttgtt atgggatttt atgatgttga agaaaacgat atttatatta acgttattct
      361 ctggcaacgt aattgctgca actgtagaat taggttttga aaatgagcaa tataattatg
      421 cttatcgttc tgcagatgtc ttcatgccgt atattaagag taatttcaac cctgttactg
      481 attctgcttt gaatgtgtca ctcacctata tgtatcagga tcaatatggg aaaaaacata
      541 aaaaaacatc tgaggacaga tttaaaacca atcgcgatcg catagagctc tatcttaaag
      601 gttatacttt aaatagggga gcatattctt tttctccttc cgcaggtttc cgttatgagt
      661 catgggatgt aaactacgat aatccgaaaa agcaggataa gtggaaactg gaactacgct
      721 tttatcctaa tatgacttat aaactcaatg accagttaag cctatatatg aatggttttg
      781 ttgcccctgt attttttaaa acacaacaag agtcgagaaa agataacaat tatgtaaagg
      841 gtaagttagg ggcgaaacgt tataacaacg attattatca ggaactccag attctgggtg
      901 tcagatataa atttaataat gataatacgc tctgggcatc agtctataat gaaagaaaat
      961 ataatcaaca ttcctcaaaa tatgatcgct ggcaattgcg tggaggctat gattttaaag
     1021 ttacagagga gtttgttttg agtccattca taagatatga cctctcttat agag
//
LOCUS       DAZKHK010000390         1070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKHK010000390 DAZKHK010000000
VERSION     DAZKHK010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.393"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(90..422)
                     /locus_tag="ACHOS5_005472"
     CDS             complement(90..422)
                     /locus_tag="ACHOS5_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9780100.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMPDTST
                     RKLVAWDGTTDGAAVGILAVDADQTSTTLMFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(432..>1070)
                     /locus_tag="ACHOS5_005473"
     CDS             complement(432..>1070)
                     /locus_tag="ACHOS5_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9780101.1"
                     /translation="PDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSG
                     QEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTD
                     VVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMT
                     VETARRIMATAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
BASE COUNT          203 a          321 c          303 g          243 t
ORIGIN      
        1 ccgccagcag ctgggcggtt gtgtacatcg acataaaaaa atcccgtaaa aaaagccgca
       61 caggcggcct ttagtgatga aggttaaggt taaacgatgc tgattgccgt tccggcaaac
      121 gcggtccgtt ttttcgtctc gtcgctggca gcctccggcc agagcacatc ctcataacgg
      181 aacgtgccgg acttgtagaa catcagtgtg gtgctggtct ggtcagcatc aaccgccaga
      241 atgccaacgg cagcaccgtc ggtggtgcca tcccacgcaa ccagcttacg ggtggaggta
      301 tccggcatca gcggggtcat tgcaggcgct ttcgcactca atcctccggg cgcggttgcc
      361 gtatgagccg ggtcactgtt gcccagcggc tggtaatggg taaaggtttc tttgctcgtc
      421 ataaacatcc cttacactgg tgtgttcagc aaatcgttaa cggcatcaga tgccgggtta
      481 cctgcagcca gcggtgccgg tgccccctgc atcagacgat ccagcgcagt gtcactgcgc
      541 gcctgtgcac tctgtggtgc agttgccata atgcggcggg ctgtttccac ggtcataccg
      601 ggggtttctg ccagcacgcg ggcctgttct tcgcgtccgt gagcctcctc acagttgagg
      661 atccccataa tgcggctgtt ttctgccgca accgcagcgg tgatctgcgc gttcacgtcc
      721 ggctgcgccg cgctggcgtt ttcgccctcc gtcgctggca ccacgtcagt aacgtcagcc
      781 tgcgaagcag tggctgaaac agttgttgat tgagtctctt tggtcattcg ccctcctgag
      841 agacgggatt tacgtgcatc cagtgcatca cgcataacgg tgatcgcatc ggtactgtta
      901 acaagttcat cagccagtcc ggcatcaatg gcctcctgac cgctgtacac tgcagcctcg
      961 gtatccagca cagcctgcac ggacaggccg gtatatgccg acaccttctg tgcaaacatc
     1021 cggcgggttg catccatccg ggactgcagt gtttcccgga catcatccgg
//
LOCUS       DAZKHK010000391         1068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKHK010000391 DAZKHK010000000
VERSION     DAZKHK010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.394"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..136
                     /locus_tag="ACHOS5_005474"
     CDS             5..136
                     /locus_tag="ACHOS5_005474"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9780102.1"
                     /translation="MTYENNKKTHQQHIEILTAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(172..>1068)
                     /locus_tag="ACHOS5_005475"
     CDS             complement(172..>1068)
                     /locus_tag="ACHOS5_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9780103.1"
                     /translation="MMLSLFNKSTIVDLSSETISESKSLSGRIYQDPEHNGEFELDRV
                     LTDIYAPQLIIFLPDKPNGTGILIIPGGGYQAVMLDKEGTALAPEFLSQGYTLFVLSY
                     RLPSPAEEENINKTFIDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGHIASTFVNQI
                     CDFKRSSDSKEQEIFAIPDFVGLIYPVISMKDDVTHPGSRKQLLGDNSTSENIIQYSA
                     DLNVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWA
                     TDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          371 a          223 c          172 g          302 t
ORIGIN      
        1 taaaatgaca tatgaaaata acaaaaaaac ccatcagcag catattgaaa tacttacggc
       61 cataaaagag catgattgtg ataaagctga acagctaatg tataatcacc tttataaacg
      121 aattgaagca tcatagttac tcgacgcttc accaggtagt caccataaat attatttaaa
      181 tccatgagaa tacatccagt ccataaacag ttttatccat cctccaacag gaagatctgt
      241 ggcccactga acactaaagc catgccctcc tttctcatag agatgcattt cagcagaaac
      301 gcctttctct attaggccac ggtacatgag cagactgttt tcaattgaca caagatcatc
      361 atcacaacaa tggagtaaaa acacaggagg aaaacatgat gtgacattaa gatccgcact
      421 atattgtatt atattttctg aggtactatt gtcccccagt aattgcttcc tggagcctgg
      481 atgtgtcaca tcatctttca ttgatattac aggataaatt aacccgacaa aatcgggaat
      541 agcgaaaatc tcttgttctt tactgtcaga agaacgtttg aaatcacaaa tctgattcac
      601 aaacgttgag gcgatatgcc ccccagcaga aaatcctaat acaccaatac gatgacgctg
      661 tagatgccat ttatcagcat tcttacgaat ataaagcatt gcagatattg catcaataaa
      721 agttttgttt atattttctt cctcagctgg agatggtaat ctgtaactaa gaacaaataa
      781 tgtatagccc tgagataaaa actctggagc aagagctgta ccttccttat ctaacataac
      841 agcctggtag ccccccccgg gaataatcaa tattcctgtt ccatttggtt tatctggtaa
      901 aaaaataatt aactgaggag cataaatatc tgtcaataca cgatcaagtt caaattcccc
      961 gttatgttca ggatcctgat aaatacggcc tgaaagagat ttactctcag atattgtttc
     1021 acttgacaag tcaacaatag ttgatttatt aaacaagctt aacatcat
//
LOCUS       DAZKHK010000392         1037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKHK010000392 DAZKHK010000000
VERSION     DAZKHK010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.395"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..90)
                     /locus_tag="ACHOS5_005476"
     CDS             complement(<1..90)
                     /locus_tag="ACHOS5_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HFP9780104.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHT"
     gene            complement(181..798)
                     /locus_tag="ACHOS5_005477"
     CDS             complement(181..798)
                     /locus_tag="ACHOS5_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780105.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(804..>1037)
                     /locus_tag="ACHOS5_005478"
     CDS             complement(804..>1037)
                     /locus_tag="ACHOS5_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780106.1"
                     /translation="TGVLTPVFFLDRFTAESGSKTYTNKPDGKSLQAVCCLFPWNNVF
                     ADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
BASE COUNT          303 a          243 c          184 g          307 t
ORIGIN      
        1 tgtatggttt ccttctgctg catgtgcaga taaatttaca caggcaatcc cggagattgc
       61 acacacaacc agtgttgcta tactcttcat ttaagatatc ctgtattcag ataacacgct
      121 aaacttttta ataatacatc tttttagaca ttttaaatga atattaaaga ctcgcttcaa
      181 ctaatcatat actgatgtat taataaccag gaccttatct atatacggct ttcgaatatc
      241 ccatgttgca ccaccggaat aatgctcaca ggaatatatt ttatttcctg tagcaccagt
      301 ggatgtggta taaatcggtc gatcataagg agttcttttc cagttataat acccgaccag
      361 tgcaggcatg attgcgcatg gatatccaag gtccttttca aatttgatat caacaggtat
      421 taacttcgcg tcaagcagca tcatttcgcc atgataaatc atctcaccgt ccgggttata
      481 catggcgata ccatactcag aaggtggagt aaccatattc gcgaaagcat aaaccgtcgt
      541 aacaccaggg ttcgttcccc tgacaattac atgaagcctt agtgcatgat atccatcaat
      601 ctgttcatgc gtgtacatga catcggcctt cttctctgtt ctgataaaga aaaaacaact
      661 tttgcctgac gggattgatg ttttaaaaaa agacttttca gtcgccggta tggtgccttt
      721 gttgatcaga cactgcggcg taaaacctgg acttatccat acgctgccat ccggcttcat
      781 aatgctcaaa ccatacatga cacttatccc cagaatgtat aaatataaga tcccataccc
      841 tgctcaagat tcgaccacgt caccgtattg ccattaatgg ttatcttcgg tactttccga
      901 tccgcaaata cattattcca gggaaataaa caacatacag cctgcaatga tttcccgtcg
      961 ggtttattcg tgtacgtctt tgagccagac tccgctgtaa atctgtccag gaaaaatacc
     1021 ggagtcaaca cgcccgt
//
LOCUS       DAZKHK010000393         1036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKHK010000393 DAZKHK010000000
VERSION     DAZKHK010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.396"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..682)
                     /locus_tag="ACHOS5_005479"
     CDS             complement(32..682)
                     /locus_tag="ACHOS5_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780107.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGGAKTPGTGANAIQHQAQNWRL"
     gene            complement(776..>1036)
                     /locus_tag="ACHOS5_005480"
     CDS             complement(776..>1036)
                     /locus_tag="ACHOS5_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780108.1"
                     /translation="PDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTR
                     LAGGRWAEFHTARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
BASE COUNT          245 a          325 c          216 g          250 t
ORIGIN      
        1 gcaagtgttt cgtatttacc gtactccata atcacagtct ccagttctga gcctgatgct
       61 gaatggcatt agcgccggtg ccaggtgttt tagcaccgcc tccgctaccg ccagccttat
      121 gcatcgcata cgcacctgcc gcacccagac cagcgccaac aagagaggct cgcccctgct
      181 gtttcgtaaa cgccgcctgt gcatccgatt tagcttttgc cagactgctg tctgccagag
      241 agttaaaact ctgtaacgca tccgccttct gaccagaacc gagagcagca acatcctgta
      301 gcccggcaac atacttatct gcctgcgaca cctgcccccg tgtggttgtg tcaatctgcc
      361 cggtcacctg gtcgctttga tttgcatcca ttaccgcatt aaaacgacca ctggatgggt
      421 caacgccgga ctgagcaaga ttacccgcca gctcccttcg cgcttcacca aactgtttct
      481 gataaccaag atttgttgtg ccagcgatat tttcgtactg ctgctcactg ttaaggtcat
      541 cgaccttttc catgaagtta tcttctgccg gacggaggat tttttgataa tcctgccacc
      601 ctttccaggc cacttcttcc tgcgctattt gcgctgctgt tggttttact ttggtatcac
      661 caccgccttt acttccaccc ataatggccc cctggataac aaaaaaccct gccggagcag
      721 ggtcagaatg tgaattacaa tgttggttta aacacaatga tgaaaactat agcagtcaga
      781 ccgctatcct gaacaccatg aagccatcct catcatccgg cattcgctca aagcccagac
      841 gttttcccag ccggataaat ccccgccttg ccgtatgaaa ttcagcccag cgtccgccag
      901 ccagacgggt taatgtcttc acctccggca gataacgctc aacgctgtta ctccccgtac
      961 acacgcccag caacaccaga acataaggga taccatcagc actgagcaca gaacgcagca
     1021 ccagaaagcc atcagg
//
LOCUS       DAZKHK010000394         1032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKHK010000394 DAZKHK010000000
VERSION     DAZKHK010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.397"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..688)
                     /locus_tag="ACHOS5_005481"
     CDS             complement(32..688)
                     /locus_tag="ACHOS5_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780109.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(782..>1032)
                     /locus_tag="ACHOS5_005482"
     CDS             complement(782..>1032)
                     /locus_tag="ACHOS5_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780110.1"
                     /translation="LVLRSVLSDDGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGG
                     RWAEFHTARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
BASE COUNT          241 a          325 c          213 g          253 t
ORIGIN      
        1 gcaagtgttt cgtatttacc gtactccata atcacaatct ccagttctga gcctgatgct
       61 gaatggcatt agcgccggtg ccaggtgttt tagcaccgcc acttcctccg ctaccgccag
      121 ccttatgcat cgcatatgca cctaccgcac ccaaaccagc gccaacaaga gaggctcgcc
      181 cctgctgttt cgtaaacgca gcctgtgcgt ccgatttagc ttttgccaga ctactgtctg
      241 ccagcgagtt aaaactctgt aacgcatccg ccttctgacc agaaccgaga gctgcaacat
      301 cctgtagccc ggcaacatac ttatctgcct gcgatacctg cccccgtgtg gttgtgtcaa
      361 tctgcccggt tacctgatca ctctggttcg cattcattac cgcgttaaag cgaccactgg
      421 acggatcaac accggactga gcaagattac ccgccagctc cttccgtgct tcgccaaact
      481 gtttctgata ccccagattt gtggtgccag caatattgtc gtactgctgc tcactgttca
      541 ggtcatcgac cttttccatg aagttatctt cagccgggcg gaggatattt ttgtaatcct
      601 gccacccttt ccaggccact tcttcctgtg ctatttgcgc tgctgttggt tttactttgg
      661 tatcaccacc gcctttactt ccacccataa tggccccctg gataacaaaa aaccctgccg
      721 gagcagggtc agaatgtgaa ttacaatgtt ggtttaaaca caatgatgaa aactgtagca
      781 gtcagaccgc tatcctgaac accatgaagc catcctcatc atccggcatt cgctcaaagc
      841 ccagacgttt tcccagccgg ataaatcccc gccttgccgt atgaaattca gcccagcgtc
      901 cgccagccag acgggttaat gtcttcacct ccggcagata acgctcaacg ctgttactcc
      961 ccgtacacac gcccagcaac accagaacat aagggatacc atcgtcactg agcacagaac
     1021 gcagcaccag aa
//
LOCUS       DAZKHK010000395         1026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKHK010000395 DAZKHK010000000
VERSION     DAZKHK010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.398"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..464
                     /locus_tag="ACHOS5_005483"
     CDS             <1..464
                     /locus_tag="ACHOS5_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780111.1"
                     /translation="ESLTAFITENLPPDAMQFFMSSQEDAQLIRSQKALGNDQIRMGV
                     MTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFNDEDSC
                     LLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            461..>1026
                     /locus_tag="ACHOS5_005484"
     CDS             461..>1026
                     /locus_tag="ACHOS5_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780112.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLELPPKKRRRLLWRIALHGIIAAA
                     RRHQRNQSAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTSGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQSGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITVMEDQIIAGPR"
BASE COUNT          234 a          290 c          325 g          177 t
ORIGIN      
        1 tggaaagcct gaccgccttc attaccgaaa atctgccgcc tgacgccatg cagttcttca
       61 tgagcagtca ggaagacgcg cagcttatcc gcagtcagaa ggcgctggga aacgaccaga
      121 tccgcatggg cgtgatgacg tacaccggcg tgattgcatg ggataacttc ccgttccgca
      181 aatactcacc ggggctggtt tacgcgctgg tcatggcctg gctggaagag cacgcggacc
      241 cgctgaggga ggaatttcgc ccggaggatg tgaacgtcga cccgcagttt aacgatgagg
      301 actcgtgcct tctcgatatc acggtgggac tggctgaccc gctgatcatc cgggtgcagg
      361 aagacggccc cgttccgtac agggggaaac gctggacact ggttaaccct gaaatctgga
      421 cggcggaaac catggatctg tttgtcagca ggagccgcga atgataagcg gccagctgaa
      481 cccgcagcag ctggcagaag cgcaggcgat gcttcagtcg ctggaactac ccccgaaaaa
      541 acgccgtcgc ctgttatggc gtattgccct gcacggcatt atcgccgcag ccagacgcca
      601 tcagcgcaat cagagcgcgc cggacggcac agcatgggca ccccggaaac gcggcaaggg
      661 gaaaatgttc agaaagctgc cgcgtcttct ggcggtccgt gagatgcccg gacaggatgc
      721 cgtaaaggtt tatttccgta acggcaatca caccagtggc aggagccgaa tcagtgccgg
      781 tgccgtgggc tgggttcacc acaacggcgc agaaatccgg gtcagtgctg acagcttcag
      841 cggtcaggac cagagcgggc agaccgccac ccgccgacag gcaaaacgac tgcgggagct
      901 gggctttaag gtacgtcaga aggggcgctg gcggaaagca tccatgaaat acctgttaac
      961 gctttcgcgg gcgcaggccg ggttgctgat caccgttatg gaggatcaga ttatcgccgg
     1021 accacg
//
LOCUS       DAZKHK010000396         1026 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKHK010000396 DAZKHK010000000
VERSION     DAZKHK010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.399"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..464
                     /locus_tag="ACHOS5_005485"
     CDS             <1..464
                     /locus_tag="ACHOS5_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780113.1"
                     /translation="ESLTAFITENLPSDAMQFFMSSQEDAQLIRSQKALGNDQIRMGV
                     MTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFRPEDVNVDPQFNDEDSC
                     LLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMELFVSRSRE"
     gene            461..>1026
                     /locus_tag="ACHOS5_005486"
     CDS             461..>1026
                     /locus_tag="ACHOS5_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780114.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRRRLLWRIARHGIIAAA
                     RRHQRDQTAPDGTAWAPRKRGKGKMFRKLPRLLAVREMPGQDAVKVYFRNGNHTTGRS
                     RISAGAVGWVHHNGAEIRVSADSFSGQDQNGQTATRRQAKRLRELGFKVRQKGRWRKA
                     SMKYLLTLSRAQAGLLITIMEDQIIAGPR"
BASE COUNT          238 a          290 c          322 g          176 t
ORIGIN      
        1 tggaaagcct gaccgccttc attaccgaaa atctgccgtc tgacgccatg cagttcttca
       61 tgagcagtca ggaagacgcg caacttatcc gaagtcagaa ggcactgggt aacgaccaga
      121 tccgcatggg cgtgatgacg tacaccggcg tgattgcatg ggataacttc ccgttccgca
      181 aatactcacc ggggctggtt tacgcgctgg tcatggcctg gctggaagag cacgcggacc
      241 cgctgaggga ggaattccgc ccggaggatg tgaacgtcga cccgcagttt aacgatgagg
      301 actcgtgcct tctcgatatc acggtgggac tggctgaccc gctgatcatc cgggtgcagg
      361 aggacggccc cgttccgtac agggggaaac gctggacact ggttaaccct gaaatctgga
      421 cggcggaaac catggagctg tttgtcagca ggagccgcga atgataagcg gccagctgaa
      481 cccgcagcag ctggcagaag cgcaggcgat gcttcagtcg ctggatctgc caccaaaaaa
      541 acgccgtcgc ctgttatggc gtattgcccg gcacggcatt atcgccgcag ccagacgcca
      601 tcagcgcgat cagaccgcgc cggacggcac agcatgggca cccaggaaac gcggcaaggg
      661 gaaaatgttc agaaagctgc cgcgtcttct ggcggtccgt gagatgcccg gacaggatgc
      721 cgtaaaggtt tatttccgta acggcaatca caccactggc aggagccgca tcagtgccgg
      781 tgccgtgggc tgggttcacc acaacggcgc agaaatccgg gtcagtgctg acagcttcag
      841 cggtcaggac cagaacgggc agacagccac ccgccgacag gcaaaacgac tgcgggagct
      901 gggctttaag gtacgccaga aggggcgctg gcggaaagca tccatgaaat acctgttaac
      961 gctttcgcgg gcgcaggccg ggttgctgat caccattatg gaagatcaga ttatcgccgg
     1021 accacg
//
LOCUS       DAZKHK010000397         1020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKHK010000397 DAZKHK010000000
VERSION     DAZKHK010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.400"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..56
                     /locus_tag="ACHOS5_005487"
     CDS             6..56
                     /locus_tag="ACHOS5_005487"
                     /inference="COORDINATES: protein motif:HMM:NF018789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9780115.1"
                     /translation="MSASQSAGSKPEEPLI"
     gene            97..342
                     /locus_tag="ACHOS5_005488"
     CDS             97..342
                     /locus_tag="ACHOS5_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9780116.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGDPTPDSDMMM"
     gene            419..634
                     /locus_tag="ACHOS5_005489"
     CDS             419..634
                     /locus_tag="ACHOS5_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9780117.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            639..863
                     /locus_tag="ACHOS5_005490"
                     /pseudo
     CDS             639..863
                     /locus_tag="ACHOS5_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
BASE COUNT          262 a          227 c          307 g          224 t
ORIGIN      
        1 agaaaatcag cgccagtcag tcagccggaa gcaaacccga agagccgctc atttagctgc
       61 aactttctta atcacacctt tcgacgagaa aatcccatgt cagaaattac atccctggtc
      121 actgcagagg cagtgaagga agtcctgcgc tctgaagaag tcctgagcgc actgaaacag
      181 aaactccgcc agaaccttga ggcgcgtctt gatgcagaag tggatgccat tctggatgaa
      241 ctgctgggcg caccggcagt tccggagccg gaaggtatcg cgggtgaggg gagtgcttca
      301 gatggcggtg accccacacc tgacagcgac atgatgatgt aagcatgcgc aagggaccat
      361 cggtgtgtgc cggtggtctt tatattgttg tgagcttccg gattgcggga gacggggtat
      421 gtaccagatg gaaaaaatca caacaggtgt gtcatacacc acgtcagcgg tggggacggg
      481 ctactggttc ctgcagttgc tggacagggt ttccccgtct cagtgggcgg caataggcgt
      541 gctggggagt ctgctgtttg gtctgctgac gtacctgacg aacctgtatt tcaagattaa
      601 agaagaccgt cgtaaggcgg cccggggaga gtaaagcgat gaataaaaaa tatgaactgg
      661 ttgtaaaagg gataaatcat tacccggata agataactgt tactgtggca ctggtaattg
      721 ccgggcagcc gtcgttgttg tcgccatatg tggcgattag tcttgaccgt actgaaggtg
      781 ccacgctgga gttttacgaa gctgaggcga aaaaacaggc gaagcagttt ttcatggatg
      841 cggctctcgg attacgcgaa gggactgcgc gatatggctt tcagaatatt tcttcatgga
      901 caatgccgga aaaaatcgcc acttacccgg ggcgtgaaaa ggtcaacgaa ccggtaaaaa
      961 atgcgcctga tgatgtgcag agcgcgatgg ttaatgtgga gcttccggca cgtgtgacac
//
LOCUS       DAZKHK010000398         1015 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKHK010000398 DAZKHK010000000
VERSION     DAZKHK010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.401"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..342
                     /locus_tag="ACHOS5_005491"
     CDS             <1..342
                     /locus_tag="ACHOS5_005491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780118.1"
                     /translation="ICFLLVFAISGCVNPYNYREEQNVIISFKTNSPPQEIQECILSE
                     WQRTPLLATITSQKIGKYYSVLAVGDNADIYKLADGSTIIDFYSLRGVLDPTNGKSKR
                     IQGIKSCISTK"
     gene            complement(449..604)
                     /locus_tag="ACHOS5_005492"
     CDS             complement(449..604)
                     /locus_tag="ACHOS5_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9780119.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
BASE COUNT          259 a          260 c          207 g          289 t
ORIGIN      
        1 atttgttttc tattggtttt tgcaatttca ggatgcgtca acccttataa ctacagagaa
       61 gaacaaaatg ttattatctc gtttaaaaca aattctccac cacaagaaat acaagaatgc
      121 attctcagtg agtggcagcg tacacctctc ttagccacta tcacgtctca aaaaattggc
      181 aagtattaca gcgtattagc ggttggagat aatgccgaca tttataagtt ggcagatggt
      241 tctactatca ttgattttta ttcccttcga ggagtcttag accccaccaa tgggaaaagc
      301 aagaggattc agggaattaa atcatgtatt agcaccaaat aatcatcagt tcgaacccag
      361 aaacagccaa atagatttta tctcaggtgc gttgaggatg cctgacacat cagaggtggc
      421 gagggatttc tccctcgcct ggtcactctt acttctcaga ttcgtagtct acgaagacag
      481 caacctccgt ctggccggtt cggattcgta cctcgcagag gtctttcctc gttaccagtg
      541 ccgtcactat gacggttaaa cagatgacga tcagggcgat taacatcgcc ttttgctgct
      601 tcatagtctg cttctccttg acctttcggt ccgtaagagg ctaacctacg tttgtgaagc
      661 atagattggg cctcagatta atgttaagcg tcctgcaaga cgcgtaatgt taactggggc
      721 ttttctctgt ctgccttacg gcggcatgcc cgaggcagac agcctcaagc acccgcagca
      781 cttctactta actcttcttt ccccgcaaac cgtttttatc cccagcggca aatcgaatac
      841 accaccagca ccagcgccac cgccattgca attcctaccg ccacgaatgc ctcaggccag
      901 gtcatcgtaa aatatcctcc gcgcttatca gtccgtttcg cttcaggtag tccatcgctt
      961 tatccggtag tttgcttgat ttattgacgc tttttaatga actggctaat cgctt
//
LOCUS       DAZKHK010000399         1007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKHK010000399 DAZKHK010000000
VERSION     DAZKHK010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.402"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(160..612)
                     /locus_tag="ACHOS5_005493"
     CDS             complement(160..612)
                     /locus_tag="ACHOS5_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9780120.1"
                     /translation="MSYIRETIRGKNEWTVYEQIGFAVSCMHYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            complement(636..>1007)
                     /locus_tag="ACHOS5_005494"
     CDS             complement(636..>1007)
                     /locus_tag="ACHOS5_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFP9780121.1"
                     /translation="VVTLIHHRHNQKLALNSSVKNDVYFTVAQQCFSQAIHWLTQGST
                     RRQINASLLLKEIEHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMK
                     NFMLKFSRDAFGYLEPIRNTV"
BASE COUNT          292 a          227 c          170 g          318 t
ORIGIN      
        1 gaaacaaaat agctcctctt actcacactt ttcctgtaat atcttcactg gttaaccgaa
       61 acgctataat caaagaaaga aaatacagag tcctgtcaaa atagtatcag cgatgcattt
      121 atcccgtaat ttaaaattat ctctgtatct tcagaagtac tacgttttat gcagccgctc
      181 agggatatat gtcacaattt tctttcgcct gcttaaccat cgtacttctc cttcccccca
      241 tggctgaagc tgtatgagat agtcaataac ttttcgacca aagcctggtt tacaacagaa
      301 atccaggatc cagatccttc catcttcatt ccattcagac ggatgcaacc taaattctgg
      361 atccctgagc aggcgcgctt ccgtatcagc ctcaagatat gcccaagtaa tatatgccag
      421 tggaaaacct cgtgaatcaa ataggaattt aatctgatta tgctgtatcg catattcagt
      481 ccagtattga atggttaaca ccggatacag actgtaatta cgattatagt gcatacaact
      541 gaccgcaaaa ccgatctgtt cataaacagt ccattcattt tttcctctga tggtttccct
      601 tatataagac atgattacta tttcccatat atttattaca ctgtgtttct tatcggttca
      661 agatatccaa atgcatctct ggaaaactta agcataaaat ttttcatatc agaaattgct
      721 tcttgtaaaa aaaggggatc atcgccatga ttatccagca ggatggcaat tttttcgcac
      781 atattctctt tcctcagctc ttccctgggt tgcagccttt tcatggtatg atgttcaatt
      841 tctttcagga gtaatgaagc atttatctga cgtctggtac tgccttgcgt cagccagtgg
      901 atagcctggg aaaaacactg ttgtgcaacg gtgaaataaa catcattctt gacgctacta
      961 ttcagtgcca gcttctgatt atgcctatga tggattagcg tcacaac
//
LOCUS       DAZKHK010000400         1005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKHK010000400 DAZKHK010000000
VERSION     DAZKHK010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.403"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1005
                     /locus_tag="ACHOS5_005495"
     CDS             <1..>1005
                     /locus_tag="ACHOS5_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9780122.1"
                     /translation="KTDGVADAPVLSIPEVTDGYANADELKDGLQAEVTLPAGTAEGA
                     VITLTVTRPDKTTENVTHTVTKDEVAAGKVSMDIPKDAVIDGQNSVSVTLTQGSNPAK
                     PGNVVDFAADTQIPGDTDGDGATDATPVVAIPEAADGVNAEELKDGVQTEVTVPKGSA
                     AGDTLTLTVTKPDGTTDTVEHTLTADEVAAGKADVTIPADKVTADGQYSVTAEITDPA
                     GNTSGQGQPADFAVDTVAPSAPVLKAEDDGSVSVELPGDANKGDTVDVTFEDEKGGKH
                     TVTLEKGDNGWTSSDPTLIPDSTGDKATIPADNVKDNSEVTGVAKDPSGNESDPSTVT
                     S"
BASE COUNT          267 a          236 c          318 g          184 t
ORIGIN      
        1 aaaaactgat ggtgtggcag atgccccggt tctgagcatt cctgaagtga ctgacggtta
       61 tgccaatgct gatgagctga aagacggttt gcaggcagag gtcacgttgc cggcaggcac
      121 ggcagaaggt gcagttatta cacttaccgt gacccgtccg gacaaaacca cggagaacgt
      181 gacacatacc gtgactaaag atgaagtggc tgccggtaaa gtatccatgg atattccgaa
      241 ggatgcggtg attgacgggc agaacagcgt cagtgtcact ctgactcagg gcagtaaccc
      301 ggcgaaaccc gggaatgtgg ttgattttgc tgccgatacc cagatcccgg gggataccga
      361 tggtgatggt gcaacagatg caacaccggt agttgccatt ccggaagcgg cagatggtgt
      421 gaatgccgaa gagctgaagg atggtgtaca gaccgaagtc acggtaccga aaggcagcgc
      481 tgccggtgat accctgacgc tgacggtcac caaaccggac ggcacgacgg acacggtgga
      541 gcacacgctg accgcagatg aagtggctgc aggtaaagct gatgtgacca ttccggcgga
      601 taaagtgacg gcagatggtc agtattctgt aaccgcggaa atcacagacc cggcaggtaa
      661 caccagtggc cagggccagc cggctgactt cgcagtggat acagtggcgc cgtcggcacc
      721 ggtattaaaa gcagaggatg atggttctgt ttctgttgag ctgccgggtg atgccaataa
      781 aggcgatacc gtggatgtca cctttgaaga tgaaaaaggt ggtaagcaca ccgtgacgct
      841 ggagaaaggg gataacggct ggacatccag tgatccgacg ctgatcccgg acagcactgg
      901 cgataaagcg accattccgg cagataacgt gaaggataac agtgaagtta ccggggttgc
      961 caaagatccg agcggtaatg aaagtgaccc gtcaaccgtg acgtc
//
LOCUS       DAZKHK010000401         1002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKHK010000401 DAZKHK010000000
VERSION     DAZKHK010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.404"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1002
                     /locus_tag="ACHOS5_005496"
     CDS             <1..>1002
                     /locus_tag="ACHOS5_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780123.1"
                     /translation="ERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRL
                     AGIVDDMLARRRELIREAGLEENGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFT
                     IGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQV
                     FTDDRPDTMRTIAERKDQETGETRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDP
                     RLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAE
                     QGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGG
                     "
BASE COUNT          261 a          254 c          308 g          179 t
ORIGIN      
        1 ggaacgtaac gcacatattg cgaaaatcaa ccctaaaatg ccggacggcg gttcggggat
       61 gaccaacgcg gaggcggcgg aaatcatgca gcgtgtgcgt aacagcggca aacaggcaca
      121 gtatgaccgt ctggcaggga ttgttgacga tatgctggcc cgtcgccgtg agcttatccg
      181 tgaggccgga cttgaagaga acggtgtggt ggatgcctgg cagaacgcct accgttacta
      241 cgttcccctg aaaggtcagg atgttgacgg tgtggtgtca cttccccgta caggtaaggg
      301 cttcaccatc ggcggacgtg aaagcaggca ggccatgggg cgtgcatccc gtgcacagtc
      361 tccgtccact caggcgatac aggacctgag cgaatcgctg atccgccatc gcaaaaacga
      421 agtgggtaac gccttcctga aactggtgca ggataatccc gacaaggatt actggcaggt
      481 attcaccgat gacagaccgg ataccatgcg gacgattgca gagcgcaagg accaggaaac
      541 tggtgaaacc cgtcgcgaag ttgtcgaacg ccctgtaccg atggcaatga tggcagaccg
      601 gtacttcacc accaaaaaga acggcaaaac gtactacatc aaactccatg acccgcgcct
      661 gatgcgtgcg atgaagaata tgggaccgga aaccagtaac gccgtaatcc gcacgctggg
      721 gaaagttaac cgcttcctgg caacggtgaa cacgtcgtat aacccggaat tcctggtcag
      781 taacttcatc cgtgacgtac agacggcggt gatgaacctg aaggcggagc agggaaggag
      841 cgacggcaaa ctgaaagggc tggataactt atccgcactg gctgtggtga aagacagtcg
      901 gtctgccatg tctgccgtat acgccagtct gcgtggtaaa accctcacgg gcaacggtgc
      961 gcagtggcag aaggtgtgga aagagtttgt tgaggacgga gg
//
LOCUS       DAZKHK010000402          995 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKHK010000402 DAZKHK010000000
VERSION     DAZKHK010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.405"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..431
                     /locus_tag="ACHOS5_005497"
     CDS             42..431
                     /locus_tag="ACHOS5_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9780124.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            451..>995
                     /locus_tag="ACHOS5_005498"
     CDS             451..>995
                     /locus_tag="ACHOS5_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9780125.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVQELTDTQNLGVGPVNTVRGGPKQGSYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVVSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLS"
BASE COUNT          241 a          229 c          293 g          232 t
ORIGIN      
        1 ctgcgtgcaa ctcactggtt acatgagagg ggcctgaaag tatgaagctt atcctgcctt
       61 tcccgcccag cgtgaacact tactggcgac accctaacaa aggggcgttt gcaggtaaga
      121 gcctgataag cgcggcgggg cgaaaattcc agagcgcggc gtgcgcagca atagttgagc
      181 agttacgtcg tctgccgaaa ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc
      241 ctccggataa caggatccgc gatctggata actataacaa ggcgctgttt gacgccctga
      301 cccacgcagg tgtgtgggaa gacgacagcc aggtgaaaag aatgctggtg gagtggggac
      361 cggttatccc ggaagggaag gtcgagatca ctatcagtaa gtacgagaaa acggcgggtg
      421 cagccgcctg attaagagga gaaacgaagt atgaataatc tgatggtcat tgatggtatt
      481 gaagttcgtc gtgatgctta tggacgttac agcctgaacg atctgcatcg tgccgcagta
      541 gcatctggtg caaatgccag aacgaaggag ccgggaaagt ttctttccag ccaacagact
      601 gttgaacttg ttcaggaatt gaccgatacc caaaatttgg gtgtcggtcc ggttaataca
      661 gttcgtggtg gtcctaagca ggggagttat gtctgcaagg aactggtgta tgcctatgca
      721 atgtggatca gtccgtcatt ccatctgaag gtgatccgta ctttcgatat ggtagtcagt
      781 gcaccggaaa aattatccgg gcaggctgct gacaagatgc aggcaggtgt gattctgctg
      841 gactttatgc gtcgggagtt aaacctgtct aactcatctg tgcttggggc ctgtcagaaa
      901 ctccaggagg ctgttggctt accgaatctg gcaccgcgct atgccattga tgctcctgct
      961 gatgcacacg atggctcaag tcgccccacg ctgtc
//
LOCUS       DAZKHK010000403          983 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKHK010000403 DAZKHK010000000
VERSION     DAZKHK010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.406"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            42..431
                     /locus_tag="ACHOS5_005499"
     CDS             42..431
                     /locus_tag="ACHOS5_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9780126.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     FEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            451..>983
                     /locus_tag="ACHOS5_005500"
     CDS             451..>983
                     /locus_tag="ACHOS5_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9780127.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAHDGSSRPTLS"
BASE COUNT          239 a          235 c          285 g          224 t
ORIGIN      
        1 ctgcgtgcag cccactggtt acatgagagg ggcctgaaag tatgaagctg atcctgcctt
       61 tcccgcccag cgtgaacacg tactggcgac accccaacaa aggggcgttt gctggtaaga
      121 gcctgataag cgcggcgggg cgcaaattcc agagcgcggc gtgtgcagca atatttgagc
      181 agttacgtcg tctgccaaaa ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc
      241 ctccggataa ccggatccgc gatctggaca actataacaa ggcgctgttt gacgccctga
      301 cccacgcggg tgtgtgggaa gacgacagtc aggtgaaaag aatgctggtg gagtggggac
      361 cggttatccc ggaagggaag gtcgagatca ctatcagtaa gtacgagaaa acggcgggtg
      421 cagccgcctg attaagagga gaaacgaagt atgaataatc tgatggttat tgatggtatt
      481 gaagttcgtc gtgatgctta tgggcgttac agcctgaacg atctgcacag ggctgctggt
      541 tctctggata agcataagcc cgcattctgg ctccgcaatg agcaaactga gcgtttaata
      601 agcgagttgc agatttgcaa ctcggtcaat atagagccag ttaacgttat tcgtggcgga
      661 aataaccagg ggacgtatgt ctgcaaagaa ctggtgtatg cctatgcaat gtggatcagc
      721 ccgtcattcc atctgaaggt gatccgtact ttcgacatgg taaccagcgc accggaaaaa
      781 ttatccggac aggctgctga caagatgcag gctggtgtga ttctgctgga ctttatgcgc
      841 cgggaattaa acctgtctaa ctcttcagtg cttggtgcct gccagaaact ccaggaggct
      901 gttggcttac cgaatctggc accgcgctat gccattgatg ctcctgctga tgcacacgat
      961 ggctcaagtc gccccacgct gtc
//
LOCUS       DAZKHK010000404          972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKHK010000404 DAZKHK010000000
VERSION     DAZKHK010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.407"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(117..821)
                     /locus_tag="ACHOS5_005501"
     CDS             complement(117..821)
                     /locus_tag="ACHOS5_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9780128.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDATQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTDDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT          267 a          222 c          189 g          294 t
ORIGIN      
        1 caataataca tttcatacag catccttgtt gatgaaataa cacctgttat tcatactcta
       61 ttaaataatt tcaatctcag gttacaactt tttgtaacct gaaatcgtat ttatatttac
      121 tcgtacagca ccacaaaact tgctgttgac ttaactacac caggtgcaac agtgctaccg
      181 ttttgagcat aacctgcagt aaaggccgcg ctgaaatcat gggtcgtagc atttcctgga
      241 attgtataag cggcggtgga ggcgctaccc tctgccgcag tagtattcag gttaatcaga
      301 ccattattcg tcgtaccctt ggagtaaatt cgaatcccta caccagtcgc ccccgcattg
      361 ccgctgttgt cagcatccgt tgatccttct acttcgttag cgagcgtttt attagcgcta
      421 tctgcagtac cggtaaaacg gaactcaatt ttctgatcat ctgtattgct acattcggtc
      481 agattaatat tgaaatcggt ctggttgctc gccccctgtg cttctacgtc gataccagtg
      541 aaaaggttgg aaggcacaac cggcaggaca acttcaatct gttttgaatc ggttgcgacc
      601 ttacaggtag ctttcactac acggccagta aagcggattt tcccccccat tgaatcaacg
      661 ttgtcatcca tatcagtgag cctgggataa tcaacggtgc tccagtcagt atccgcccca
      721 ccaaattgtg ttgcatcgcc gggtgctgcg gttaccgcag tagacatcag catacctgat
      781 gccagtaata ctgcggataa attagataaa ttgaatttca tttatacctt tccttttgtt
      841 aaaaaatcca agcgacattg gtgtttttac attctgcgtt aataagttat atctcacaaa
      901 aaaaaataag ctgacgttgg ctaattccag aaggttcttt agaagagtaa ttgagtgaat
      961 aatattatat at
//
LOCUS       DAZKHK010000405          960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKHK010000405 DAZKHK010000000
VERSION     DAZKHK010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.408"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..318
                     /locus_tag="ACHOS5_005502"
     CDS             67..318
                     /locus_tag="ACHOS5_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP9780129.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            329..>960
                     /locus_tag="ACHOS5_005503"
     CDS             329..>960
                     /locus_tag="ACHOS5_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780130.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTG"
BASE COUNT          257 a          203 c          286 g          214 t
ORIGIN      
        1 gcttctgcac cggcaagaca gaaactgagt gttggcggac aactggtggc agtgtaaggg
       61 ggcaatatgg gatacggttt actggatatt gcaaatcagt cgcggcgtga ggcattacag
      121 ggaataagtg acgcagacag acgacgcaca gagattgagg catcaaataa acaactggct
      181 gctcaacaga aagctcagaa aaaacagaat atcggtacgg gcattggtac gggggcggct
      241 attggcgcat ccgttggtgg tcctgttggc gctgttgctg gtgcagtaat tggcggcatt
      301 gctggttctt tgttttaagg agtggtgaat gagcggattt gcacaggggt tacttgccgg
      361 attcagcact gttgaccagg caatgacccg tcgtaaggag cttggtttgc gagaagcaca
      421 gcttgctcag caacagaaaa ataacgagcg cgattttgaa tttgcgcagt ctcagtttga
      481 acataataaa gacgttgatc agcggaactt tgattacaga gccaaagttg atgaccgcaa
      541 ttacacactg caggaaaggg agtttaacgc caaccagaat taccggaatg cgtcgctggg
      601 gatggaacag caacgactcc agttgcagaa atacaaccag cgacggcttg agtataacga
      661 tatgctggcg cgcgatcagc ctgtgatggc ggcgctggga aaagcgattg atgcgggtga
      721 ccatgatgcg gcttctcatc tgttcggtaa attatcggac gccaatccgc ttaagatgat
      781 gtcaacggta ggctatgctg cgaaagcggg tcaggccgtg aacaacctgc agaaaatctt
      841 tgatgataag ccggacaggg cgattgcttc gctgaatacc ccggaaaatc tcgatgtgct
      901 ttccggcgtg tttgccccgg aactacaaca gcgtattggc atgcctgatt caaccgggga
//
LOCUS       DAZKHK010000406          955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKHK010000406 DAZKHK010000000
VERSION     DAZKHK010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.409"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..381
                     /locus_tag="ACHOS5_005504"
     CDS             <1..381
                     /locus_tag="ACHOS5_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9780131.1"
                     /translation="SCMLYNRNYSLYPVLTIQYWTEYAIQHNQIKFLFDSRGFPLAYI
                     TWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWILDFCCKPGFGRKVIDYLIQLQPWG
                     EGEVRWLSRRKKIVTYIPERLHKT"
     gene            534..>955
                     /locus_tag="ACHOS5_005505"
     CDS             534..>955
                     /locus_tag="ACHOS5_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HFP9780132.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTL"
BASE COUNT          283 a          160 c          233 g          279 t
ORIGIN      
        1 agttgtatgc tctacaatcg taattacagt ctgtatccgg tgttaaccat tcaatactgg
       61 actgaatatg cgatacagca taatcagatt aaattcctgt ttgattcacg aggttttcca
      121 ctggcgtata ttacctgggc atatcttgag gctgatacag aagcgcgcct gctcagggat
      181 ccagaattca ggttgcatcc gtctgaatgg aatgaagatg gaaggatttg gattctggat
      241 ttctgttgta aaccaggctt tggtcgaaaa gttattgact atctcataca gcttcagcca
      301 tggggagaag gagaagtacg atggttaagc aggcgaaaga aaattgtgac atacatccct
      361 gagcggctgc ataaaacgta gtacctctga agatacagag ataattgtaa attacggggt
      421 aaatgcatcg ctgatactat tttgacagga ctctgtattt tctggctttg attatagcat
      481 ttcgcttaac cagtgaaggt attacaggaa aagtgtgagt aagaggagct attttgtttc
      541 gtcaggatgc tttagaaaac agaaaaatga agtggcaggg acgggcaata ttacttcccg
      601 gaataccact atggttaatc atgctgggaa gcatagtgtt tattacggca tttctgatgt
      661 tcattattgt tggtacctat agccgccgtg ttaatgtcag tggtgaggtc acaacctggc
      721 caagagctgt caatatatat tcaggtgtac agggatttgt tgtcaggcag tttgttcatg
      781 aaggacagtt gataaaaaaa ggggatcctg tttatctgat tgacatcagt aaaagtacac
      841 gtagtggtat tgtcactgat aatcatcgtc gggatataga aaaccagctg gttcgtgtgg
      901 acaacattat ttcccgtctg gaagaaagta aaaaaataac gctagatacc ctgga
//
LOCUS       DAZKHK010000407          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKHK010000407 DAZKHK010000000
VERSION     DAZKHK010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.410"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            630..>943
                     /gene="subA"
                     /locus_tag="ACHOS5_005506"
     CDS             630..>943
                     /gene="subA"
                     /locus_tag="ACHOS5_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP9780133.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHR
                     VYG"
BASE COUNT          267 a          182 c          203 g          291 t
ORIGIN      
        1 taagaatttc agcgtctgaa attggtgttg ctctagaagc tgaaaaaata ggtgaaattg
       61 aaaaaataaa aatgccacaa ataagaaaag atgtaaatta aggaaacaac atgttgggtg
      121 gagcggacaa tccaaatggc gaattaccgt cttatatcac tggcgctgac acctgatatc
      181 cggggcttac tgcacacata ataatgagcc agggtaaacg acacaaccat gcgctgatcg
      241 ctccggtaag acggcactgt aatttcctgt tcgccatgat gcatgacggt actctttatt
      301 gtcataaaca tgccaggcaa catagttgcc ccctcttatt ttttctggct aatttttacc
      361 catcatagct ggatttactt ctgctctgaa ggagtaggaa gagcatctca ccgattacct
      421 attgcagaaa tcctggtgca tattaccgtg aaggagttca gtgagctatt gaatatgcaa
      481 ataaataaat taatattttt gagaatgact tcactcgctt tattgattgt gattgtttat
      541 cttttgtcga aaacaggata tttcagatgt gatgttcctg taagatattg acctgcaata
      601 ctgtttattt gtactgactg gaggatattg tgcttaagat tttatggccg catattctgt
      661 tcctgctttt tatatctgtg tctgtcaggg cagaaaaacc ctggtatttt gatgcaattg
      721 gcctgacaga aacaaccatg tcgctcacag acaagaatac cccggttgtc gtatctgttg
      781 ttgattccgg agtggcattt gtaggaggcc tcagcgacag cgaatttgcc aaattcagtt
      841 ttactcagga tggttcgcca ttcccggtaa aagagtctga agctttatat attcatggta
      901 ctgccatggc ttcactcatt gcctcacgtc atagggttta tgg
//
LOCUS       DAZKHK010000408          942 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKHK010000408 DAZKHK010000000
VERSION     DAZKHK010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.411"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..769
                     /locus_tag="ACHOS5_005507"
     CDS             <1..769
                     /locus_tag="ACHOS5_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP9780134.1"
                     /translation="FNTSIIVYLIDPKYFADLPTSQFWSYATYFRVLSFEYLSESIST
                     LLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGV
                     IYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYT
                     LKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYP
                     LKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            779..>942
                     /locus_tag="ACHOS5_005508"
     CDS             779..>942
                     /locus_tag="ACHOS5_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFP9780135.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYG"
BASE COUNT          318 a          164 c          168 g          292 t
ORIGIN      
        1 atttaatacc tcaattattg tatatttaat tgacccaaaa tactttgctg atctgccgac
       61 gtcacagttt tggtcgtacg cgacatactt cagggtattg tcctttgaat atctgagtga
      121 aagtatttcc acactgctgt atctggatgc cgatgtcgtt tgtaaaggaa gtctgaaacc
      181 tctcacaaaa attatattta aagatgagtt tgctgcggtt attcctgaca atgatagtac
      241 tcaggcggca tgtgcaaaac gtcttaacat tcccgaaatg aatggacgtt atttcaatgc
      301 aggcgttatc tatgtcaatc ttaaaaaatg gcatgaagca aatttgacac cgtatttact
      361 cacgctttta cgaggggaaa ctaaatatgg ctctcttaaa tatttagatc aggatgcgtt
      421 gaatatcgca tttaatatga ataatatcta cctcgcgaag gattttgata ctatttatac
      481 cctgaaaaac gaacttcatg atcgtagtca tcgaaagtat cagcaaacca ttaccgataa
      541 aacagtattg attcactata cagggataac taaaccatgg catagctggg ctggatatcc
      601 gtctgcatca tactttaata tcgcgcgtga acaatctccc tggaagaaat atcctcttaa
      661 agaggcgcgg actgttgcag aaatgcagaa acaatataag catctgtttg cccatggtga
      721 gtatattaaa ggtataactt cattaattaa gtacaagctt aagaaataaa ttactcttat
      781 gattacaagt atacgctatc gcggcttctc attttattac aaagataacg ataataaata
      841 taaagaaatc tttgatgaaa tactggccta taattttaaa acagtaaaag tattacgtaa
      901 tattgatgat accaaagtat cgttgattga tacgaaatat gg
//
LOCUS       DAZKHK010000409          940 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKHK010000409 DAZKHK010000000
VERSION     DAZKHK010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.412"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>940)
                     /gene="mchF"
                     /locus_tag="ACHOS5_005509"
     CDS             complement(<1..>940)
                     /gene="mchF"
                     /locus_tag="ACHOS5_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFP9780136.1"
                     /translation="ELGALKMPCILHWDFSHFVVLVSVKRNRYVLHDPARGRRYLGRE
                     EMSRYFTGIALEVWPGSEFLAETQQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAIN
                     LVMPVGTQLVMDHAIPAGDRGLLTLISAGLMFFILLRAAVSMLRAWSSLVMSTLINIQ
                     WQSGLFNHLLRLPLAFFERRKLGDIQSRFGSLDTLRATFTTCVVGAIMDSIMVVGVFV
                     MMLLYGGYLTWIVLGFTMVYVLIRLVTYGYYRQISEETLVRGARASSYFMESLYGIAT
                     VKIQGMAGIRGTHWLNLKIDAINSGIKLTRMDLLFGG"
BASE COUNT          276 a          259 c          196 g          209 t
ORIGIN      
        1 cccccgaaga gcaaatccat cctggttaac ttaatacctg aattgatcgc atctattttc
       61 aggttaagcc agtgtgttcc ccgaatccca gccataccct ggatttttac cgtggcaata
      121 ccatacaggc tttccataaa ataggagctg gcccgggccc ccctgacaag agtttcttcc
      181 gatatttgcc ggtaatagcc gtatgtcacc agacgaataa gaacgtaaac catggtaaaa
      241 ccgagcacta tccaggtaag atatcctcca tataacagca tcatcacaaa aacacccaca
      301 accataatac tgtccatgat tgccccaaca acacaggtag taaaggtggc cctcaaagtg
      361 tcaagggagc caaaacgcga ctggatatca cctaatttac ggcgttcaaa aaacgccagc
      421 ggcagtctga gaagatggtt aaacagaccc gactgccact gtatattgat gagcgtgctc
      481 ataaccagtg aggaccatgc acgcagcata ctcaccgcgg ccctgagcaa tataaagaac
      541 atcaggccag cagaaataag cgtcagcagc cctctgtccc ccgccggaat cgcatgatcc
      601 ataaccagct gagttcccac cggcattacc agattgattg cttcaattac aactgacaga
      661 cagaaaattt tcgccagtgt tcttttaata ccgtaaatac tgttaatcag tgaacggaga
      721 cttatgcggg tctgctgggt ttccgccagg aattcacttc cgggccagac ctcaagtgca
      781 atgcccgtaa aataccggct catttcctcc cgaccgagat atcttctgcc tctggccgga
      841 tcatgcagta cataacggtt acgctttacg ctgaccagca cgacgaagtg actgaaatcc
      901 cagtggagaa tacacggcat tttgagggca ccaagttcat
//
LOCUS       DAZKHK010000410          912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKHK010000410 DAZKHK010000000
VERSION     DAZKHK010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.413"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..309
                     /locus_tag="ACHOS5_005510"
     CDS             64..309
                     /locus_tag="ACHOS5_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780137.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEEVATINIKSGEQAA"
     gene            306..755
                     /locus_tag="ACHOS5_005511"
     CDS             306..755
                     /locus_tag="ACHOS5_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9780138.1"
                     /translation="MTAQISVYGRLVDDPQTKQTSKGTPMTLARMAVSLPCSQSDDGQ
                     ATMWLSVLAFGRQADALAKHHKGELLSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTVRPGGKKGQQGQATDALNRAKQQADQQGSHPPVGDNEQWGDDIPF"
BASE COUNT          287 a          206 c          252 g          167 t
ORIGIN      
        1 aaaggtgaac aggtgaaggg ttaaaacgca aaaattttaa tttactgctg tgagataaag
       61 cctatgacag cgaagcacac aaaaaaatca caatcgcacg cccttgattt gacggaacac
      121 tggttaaggg tgtcgataaa aatcatcgac cgcaacgccg gggaaggata cgcgaaagca
      181 catcccgaac tgattagcgc attcatgaca acggcggctg caaactttgc cactctgacc
      241 gaacgggaga ttgctgaagc ggaggaagtg gcaacaatca atattaagtc cggagagcag
      301 gcagcatgac ggcgcaaata tcagtttacg ggcggttggt ggacgacccg cagacaaaac
      361 agaccagcaa gggcaccccc atgacgctgg cgcgtatggc ggtatcactg ccctgcagtc
      421 agtcggatga cggtcaggcg acgatgtggt tatctgtcct ggcgtttggc agacaagccg
      481 acgcgctggc aaagcatcac aaaggcgaac tcctgagcgt ggcgggtaat atgcaggtga
      541 gccagtggac tggacagaac ggtgaaacgc ggcagggctg gcaggttatc gcagacagcg
      601 taatcagtgc gcgaacggtg cgaccgggcg gcaaaaaagg tcaacagggt caggctactg
      661 acgcactgaa cagagcaaaa caacaggcag atcagcaagg aagccatcca ccagtgggag
      721 ataatgaaca atggggagat gatattccgt tttaaatatt gccaataaaa aaggccggaa
      781 aaaaataaat tttccggcat gctacataaa tcccgaccaa agggagtgaa gatattaaca
      841 ctaattatcc gcgctgaagt tgttatccca aaactttata caacattgca ctcggttgca
      901 tgtattcgca tg
//
LOCUS       DAZKHK010000411          902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKHK010000411 DAZKHK010000000
VERSION     DAZKHK010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.414"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..441
                     /locus_tag="ACHOS5_005512"
     CDS             <1..441
                     /locus_tag="ACHOS5_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFP9780139.1"
                     /translation="ALIPGIQTQWMSAGSQYNPYLKISYQATPDLNLALRYRYDWKAF
                     RQTDLDGNRARNDQHQFDGYVTYKINDSWLFAWQTTVYTKVNNFKYGNHKKTATENAF
                     VLQYKMSPVFTPYIEYDYLDKQGVYKGKDNKHENSYRVGVLINF"
     gene            454..>902
                     /locus_tag="ACHOS5_005513"
     CDS             454..>902
                     /locus_tag="ACHOS5_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFP9780140.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIENGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNE"
BASE COUNT          304 a          143 c          201 g          254 t
ORIGIN      
        1 gctctaatcc cagggattca gacacaatgg atgtcagcag gaagtcagta taacccttat
       61 ctgaaaattt cgtatcaggc aacgccagat cttaatctgg ccttacgcta tcgctatgac
      121 tggaaagctt tccgacagac ggatttagat ggcaacaggg cgcgtaatga tcagcatcag
      181 tttgatggtt atgtgacata caaaattaat gattcatggt tgtttgcatg gcagaccacg
      241 gtatatacga aggttaataa tttcaaatat gggaatcata aaaaaacagc aactgaaaat
      301 gcatttgttc tgcaatataa aatgtcaccg gtgtttaccc catatataga atatgattac
      361 ctggataaac aaggggtgta taaggggaaa gataataaac atgaaaatag ttaccgtgtc
      421 ggtgttctga ttaattttta aaaggtgatt acaatgaaag tgaaaaactt tatatatctt
      481 cccttctgct tgtttattgg aacgtcggta gccggagctt taccggaaat tcctgaacca
      541 tttaaatatg gtgtaggagg gatcgagaat ggtaaaatat atataggact gggaagtctg
      601 ggaaataact ggtatatgat agatacaaac cagagtgaaa aaaaatggac taagatagca
      661 caatggccga cagttcccag agagcaggca acagctacta ttattgatgg taagatctat
      721 gtatttggag gaattggaaa agataaaagt ggggtcataa ccttacaaaa agatgtctat
      781 tcctatgata ttgctaaaga taaatgggaa aaattgatga cccgtccccc tgtatctctt
      841 gccggtcatg tttcttttat ccataatggt catgcggtat ctactggtgg tgtaaatgag
      901 aa
//
LOCUS       DAZKHK010000412          893 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKHK010000412 DAZKHK010000000
VERSION     DAZKHK010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.415"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..320
                     /locus_tag="ACHOS5_005514"
     CDS             69..320
                     /locus_tag="ACHOS5_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP9780141.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            331..>893
                     /locus_tag="ACHOS5_005515"
     CDS             331..>893
                     /locus_tag="ACHOS5_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780142.1"
                     /translation="MSGFAQGVLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHRKENDQRTYDLNVRNADREYALKEREHKAAQNYRNASLGLSQQRLQLEKY
                     NQRRIEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENL"
BASE COUNT          251 a          182 c          271 g          189 t
ORIGIN      
        1 gcttcggcac cggcaagaca gcaactgagt gttggcggac aactggtgtc agcgtaaggg
       61 gggaatatat ggggtacggt ttactggata ttgcaaatca gtcgcggcgt gaggcattac
      121 agggaataag tgacgcagac cgacgacgtg aagaaattga ggctgcgaac aaacagatgg
      181 cggcgcaaca gaaagcgcag aacaagcaga atatcggtac gggcattggt acgggggcgg
      241 ctattggcgc atccgttggt ggtcctgttg gtgctgttgc tggagcagta attggcggca
      301 ttgctggttc tttgttttaa ggagtggtga atgagcggat ttgcacaggg ggtacttgcc
      361 ggattcagca ccgttgacca ggcaatgacc cgtcgtaagg agcttggttt gcgtgaggca
      421 cagcttgctc aacaacagaa aaataacgag cgcgattttg aatttgcgca gtctcagttt
      481 gagcaccgga aagagaatga tcagcgtaca tatgatctta atgtcaggaa tgctgaccgt
      541 gaatatgcgc tgaaagagag ggaacacaaa gcagcgcaga attatcgaaa tgcgtcactt
      601 gggctatctc agcagagact gcaactggaa aaatacaacc agcgacggat tgagtataac
      661 gatatgattg cccatagcca gccactaatg gaagcgcttg gaaaagcaat tgaggctggc
      721 gatcaggagg ctgcaacgcg tctgttcggg cagctgccaa agggacatcc attaattctt
      781 atgtcaaacg aaggctatgc agcgaaagcg ggtcaggccg tgatcaacct gcaaaaaatc
      841 tttggtgata agccggacat ggcgatcgat tcgctgaata ccccggaaaa tct
//
LOCUS       DAZKHK010000413          892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKHK010000413 DAZKHK010000000
VERSION     DAZKHK010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.416"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..527
                     /locus_tag="ACHOS5_005516"
     CDS             54..527
                     /locus_tag="ACHOS5_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9780143.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            543..>892
                     /locus_tag="ACHOS5_005517"
     CDS             543..>892
                     /locus_tag="ACHOS5_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFP9780144.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTP"
BASE COUNT          204 a          268 c          230 g          190 t
ORIGIN      
        1 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaaa
       61 cattgtctca gaacaccacc tcttcggcct gtgcaccgga gactggcctg caacaactgg
      121 ttgccaccat agtccctgat gaacagcgca tcagcttctg gccgcagcat tttggcctca
      181 ttccacagtg ggtgaccctg gagccccgtg tcttcggctg gatggaccgt ctgtgcgaaa
      241 actactgcgg tggtatctgg aatctgtaca ccctgaacaa cggcggggca tttatggccc
      301 ctgaaccgga tgacgatgat gacgaaacat gggtactgtt caatgccatg aacggtaacc
      361 gcgctgaaat gagcccggaa gccgccggca ttgccgcctg tctgatgacg tacagtcatc
      421 acgcctgccg tacggagtgt tatgccatga cggttcatta ttaccgtctg cgggattacg
      481 ccctgcagca tccggaatgc agcgccatta tgcgcatcat cgactgaatg gaggcaggga
      541 caatgcagca gctttccttt ctgcccggag aaatgacaac cagagagcgc agcctcattc
      601 tgcgggccct gaaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca
      661 cccgtgcggc acgggaatgg ctgattctga acatggcggg actggagcgt gaagagttcc
      721 gggtgctgta tctgaataac cagaatcagc tgattgccgg tgaaaccctc ttcaccggca
      781 ccatcaaccg cacggaagtc catccccggg aagtgattaa acgcgccctg tatcacaatg
      841 ccgcagccgt ggtgctggca cataatcacc cgtccggtga agtcacaccc ag
//
LOCUS       DAZKHK010000414          889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKHK010000414 DAZKHK010000000
VERSION     DAZKHK010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.417"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..524
                     /locus_tag="ACHOS5_005518"
     CDS             54..524
                     /locus_tag="ACHOS5_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9780145.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCENYCGGIWNLYTLNNGGAFMAPEPDDDDETWILFSAMNGNR
                     AEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            540..>889
                     /locus_tag="ACHOS5_005519"
     CDS             540..>889
                     /locus_tag="ACHOS5_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFP9780146.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTP"
BASE COUNT          198 a          266 c          230 g          195 t
ORIGIN      
        1 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaaa
       61 cattgtctca gaacaccacc tcttcggcct gtgcaccgga gactggcctg caacaactgg
      121 ttgccaccat agtccctgat gaacagcgca tcagcttctg gccgcagcat tttggcctca
      181 ttccacagtg ggtgaccctg gagccccgta tcttcggctg gatggaccgt ctgtgcgaaa
      241 actactgcgg tggtatctgg aatctgtaca ccctgaacaa cggtggggca tttatggccc
      301 ccgaaccgga tgacgatgat gaaacctgga tactgttcag tgccatgaac ggtaaccgcg
      361 ctgaaatgag cccggaagcc gccggtattg ccgcctgtct gatgacgtac agtcatcacg
      421 cctgccgtac ggagtgttat gccatgacgg ttcattatta ccgtctgcgg gattacgccc
      481 tgcagcatcc ggaatgcagc gccattatgc gcattattga ctgaatggag gcagggacaa
      541 tgcagcagct ttcctttctg cccggagaaa tgacgcccgg cgagcgcagt ctcattctgc
      601 gggctctgaa aaccctggac cgccatcttc atgaacccgg tgtggccttc acctccaccc
      661 gtgcggcacg ggaatggctg attctgaaca tggcgggact ggagcgtgaa gagttccggg
      721 tgctgtatct gaataaccag aatcagctga ttgccggtga aaccctcttc accggcacca
      781 tcaaccgcac ggaagtccat ccccgggaag tgattaaacg cgccctgtat cacaatgccg
      841 cagccgtggt gctggcacac aatcacccgt ccggtgaagt cacccccag
//
LOCUS       DAZKHK010000415          886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKHK010000415 DAZKHK010000000
VERSION     DAZKHK010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.418"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(153..716)
                     /gene="ipbA"
                     /locus_tag="ACHOS5_005520"
                     /gene_synonym="fimX"
     CDS             complement(153..716)
                     /gene="ipbA"
                     /locus_tag="ACHOS5_005520"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFP9780147.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          273 a          168 c          172 g          273 t
ORIGIN      
        1 tttgtgatta atcatttcaa atcatggggt tattttactt tagctagaaa tatcaacatg
       61 ttatgtgttt tatttaattg aatgggctgt ttttttatgg aagaattgag gaaaacgtag
      121 gaatctgaca ttttgaatca gaaggtactg ttttaacgag gctttttttt ccacacccct
      181 ttaaaacgag cagcattact ggctgtgtat ctgacagtat gctgaatatt tcgatgaccc
      241 agataatcct gaagtagtcg ggtatcgaca ccattgtcag ccagagcaaa accacaggca
      301 tgacgtaaca tatgaggatg tgcttttaca gctaatcctg cacgttttcc agcctcacga
      361 atgatagaaa aaaaatgttg tctgctgata gggcggcgtt tccgggagag aaaaagccag
      421 tctccttcaa ctccgttttc aattaacttc ctctgcttaa gccacaactt gatcaaatta
      481 tattcatcag gaagaagagg atgtgttgtc gagaaaccat ttttaatacg ccgaatatta
      541 agttgttttc cagatgcatc aatatcggat aaacgtaaat caagaagttc actggcccta
      601 aaaccgtgaa tgaaagccat cataatcaga cagcgatttc tttcaggaaa ggacatgctc
      661 tgggctgcat ccatcagcct gtagacttca tcctgtgtga gatattttct cgtcatacgc
      721 caccgtgtac agttgtaaaa aaagagaggc acacccagat aagtatgcct gcaatcagaa
      781 cgttatcgat gtaatctcat tatctcatta ataagatttt ttgaatagta gttctacgca
      841 acagattaaa tatgaactat ttattgtgca gcttttctac aagtca
//
LOCUS       DAZKHK010000416          877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKHK010000416 DAZKHK010000000
VERSION     DAZKHK010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.419"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..610)
                     /locus_tag="ACHOS5_005521"
     CDS             complement(<1..610)
                     /locus_tag="ACHOS5_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780148.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDALVNNPVKTLAGLSSFMTDVYMPPS
                     STQKELGAPPSVGDAIKNIPDGTGKNAVTDTVGKGLKVTGKSVSDGAKASDEWLTGKM
                     SPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVVGGVA"
     gene            complement(696..>877)
                     /locus_tag="ACHOS5_005522"
     CDS             complement(696..>877)
                     /locus_tag="ACHOS5_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780149.1"
                     /translation="KQEFVKEAAANAPALLEPGREKEVEIYYQNHLRDKKAEHEKALD
                     YSASAAAEKIKGWMK"
BASE COUNT          207 a          254 c          195 g          221 t
ORIGIN      
        1 tagccacacc gccaacaacc atatcaggta caagcgcacc aatcaggttt acacccttcg
       61 ccacccagac cgcagaatca tcatagcctt cggtcatcgg cgtattcagc gcacgaacag
      121 cacccggcga catcttaccg gtcagccatt catccgaggc tttagcacca tcactgactg
      181 acttgcctgt gaccttcaga cctttaccga cagtatcagt caccgcgttt ttccctgttc
      241 catcgggaat gtttttaatc gcatctccaa cagaaggtgg tgcgcccagt tccttttgcg
      301 tggaggaagg cggcatgtaa acatccgtca tgaaagacga caacccggca agcgttttta
      361 caggattatt tacaagtgca tcaccgagtc cacgggccat ttcccctgtt ccccggacgg
      421 actgggcgaa acctttaccc attgttggca atacatcacc gaggctgaac gatgtgctgt
      481 gatcaatggc attctgcggg ttgctgaaaa aatcctgcca gtaattttct cccggctgct
      541 taatgttcag gctgctgcgg ttctggttac ccaattgcgc ttcagggcgc tgctcatctg
      601 aaaaagccat acagactcca gtaaaaaaaa gcccgacacg atggtcgggc gtaagtgaac
      661 gatattaacg gggagcgcgt tactgccgca tatctttact tcatccagcc tttgatcttc
      721 tcggcagctg cggaagcgct ataatccagc gccttttcat gctctgcctt tttatcccgg
      781 aggtgattct ggtaataaat ttccacctcc ttttcacgac caggttccag caaagcaggc
      841 gcattggcgg cagcctcttt tacaaactcc tgtttaa
//
LOCUS       DAZKHK010000417          865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKHK010000417 DAZKHK010000000
VERSION     DAZKHK010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.420"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>865)
                     /locus_tag="ACHOS5_005523"
     CDS             complement(<1..>865)
                     /locus_tag="ACHOS5_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFP9780150.1"
                     /translation="PAHFERAPYFQVKPEYSSQLIHDACRVRSVIHKLPGYLPVPPEE
                     IDAEARTDPRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLS
                     CLFPGDSPADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEI
                     FQKTFHEACYYCYLELLGIPGEFITQKEIPDILKQLVNSFVALLNKYCEKSHAQDIVN
                     QDGSPLQKLVVTLIHHRHNQKQALNSSIKNDVHFTVAQQCFSQAIHWLTQGSTRRQIN
                     ASLLLKEIEHHT"
BASE COUNT          246 a          187 c          178 g          254 t
ORIGIN      
        1 tggtatgatg ttcaatttct ttcaggagta atgaggcatt tatctgacgt ctggtactgc
       61 cttgcgtcag ccagtggatg gcctgggaaa aacactgttg tgcaacggtg aaatgaacat
      121 cattcttgat gctactattc agtgcctgct tttgattatg cctatgatgg attagcgtca
      181 caaccaactt ttgcaatggt gaaccatcct gattgacaat atcttgggca tgtgatttct
      241 cgcagtattt attgagtaat gcaacaaaac tgttaaccag ttgtttcaat atatcaggta
      301 tctctttttg tgttataaat tctccaggaa tacccaacag ttctagataa cagtaataac
      361 atgcttcatg aaaagttttc tgaaatatct cctcaacctt caaacgggct gcaagagtga
      421 agtcagggtc aaacatatat tttccgatat acaagcagag atcagacagc ccttgcataa
      481 gtaccatcgg ttcttttgcc agttcagcta atcttagttt atacccaaaa tcagctgggg
      541 aatctcccgg aaacagacaa ctgagtccgt taaaatccat taatctgcca tccattgcaa
      601 tattagaagg agaagttgtc aatctcagga aacgtgttcg acaaaatgcc atttgccagg
      661 cctcacgacg tgccagttca cataacccct caatgcaata aatccgggga tcagttcgtg
      721 cttcagcatc aatttcttcc ggtggtacag gtagatatcc tggcagcttg tggatcacag
      781 atctaacccg acaggcatcg tgaattaact gactggaata ctccggtttt acttggaagt
      841 atggtgcccg ttcaaaatgc gccgg
//
LOCUS       DAZKHK010000418          859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKHK010000418 DAZKHK010000000
VERSION     DAZKHK010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.421"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            181..783
                     /gene="fimB"
                     /locus_tag="ACHOS5_005524"
     CDS             181..783
                     /gene="fimB"
                     /locus_tag="ACHOS5_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP9780151.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          258 a          162 c          181 g          258 t
ORIGIN      
        1 gtatggcaaa gttattataa gtaactatcg ctactccatt taagttcact cagaagaact
       61 ggtccactta cgttagttat taagcaaacg ttcgttttta taaacataat caggattaaa
      121 atgttggatt attgctaacc cagcacagct agtgcgcgtc tgtaattata agggaaaacg
      181 atgaagaata aggctgataa caaaaaaagg aacttcctga cccatagtga aatcgaatca
      241 ctccttaaag cagcaaatac cgggcctcat gctgcacgta attattgtct gacattactt
      301 tgttttattc atggtttccg ggcgagtgaa atttgccgat tgaggatttc ggatattgat
      361 cttaaggcaa agtgtatata tatccatcga ttaaaaaaag gcttttcaac aacgcacccg
      421 ctattgaaca aagaaattca ggctttaaaa aactggttga gtatccgtac ttcttatccg
      481 catgctgaga gcgagtgggt atttttatca cgtaagggga atccactttc tcggcaacag
      541 ttttaccata ttatctcgac ttccggtggt aatgccgggt tgtcactgga gattcatccg
      601 cacatgttac gccattcgtg tggttttgct ttggcgaata tgggaataga tacgcgactt
      661 atccaggatt atcttggaca tcgcaatatt cgtcatactg tctggtatac cgccagcaat
      721 gcagggcgtt tttacggcat ctgggataga gccagaggac gacagcgtca cgctgtttta
      781 tagagatagt tgagatacca gggatggtgt taaaaactac attattctta cgatatatca
      841 aaaatgatta accatttat
//
LOCUS       DAZKHK010000419          857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKHK010000419 DAZKHK010000000
VERSION     DAZKHK010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.422"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>857
                     /locus_tag="ACHOS5_005525"
     CDS             <1..>857
                     /locus_tag="ACHOS5_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9780152.1"
                     /translation="SMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITANGDK
                     GLKISLNNDLSVGSNNTPGSIKVNGADGKEAVSLNGQDGTIKLTSAPAANGKSASATI
                     AVNAGKGGLTDAAGTTKTRLTYTPDGKSPAEELATMNDGLSFEGDDGKVIHKKLNEKL
                     TIKGGAEKNAEVTDGNIRVDNDNGGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITI
                     TTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKN
                     ALRDSSAAL"
BASE COUNT          247 a          181 c          248 g          181 t
ORIGIN      
        1 ccagtatgca gggtgcaggt ttcgcactga aagactctga tgataaggaa gtgaaacagt
       61 ctcttgggca ggcgattaaa gtatccgggg acagcaatat caccaccaca gcaatcactg
      121 ccaatggaga taaggggtta aaaatatccc tgaataatga cctgagcgtt gggagcaata
      181 atactcccgg cagcatcaaa gttaacgggg ctgatggtaa ggaggctgtg agtctgaatg
      241 gtcaggatgg cacgattaaa ctgacatccg cgcctgctgc gaacggaaag tctgcgtcag
      301 caaccattgc tgttaatgca ggcaaaggtg gattaacaga tgctgccggt accacgaaga
      361 cacgtctgac ttataccccg gatggaaaat caccggcaga agagctggcg accatgaatg
      421 acggtctttc ttttgagggt gatgacggaa aggttattca taaaaaactg aatgaaaaac
      481 tgaccatcaa aggtggtgca gagaagaatg ctgaagtgac tgacggcaat atccgggttg
      541 ataatgataa tggcgggctt ctggtgagga tgaccagaaa cctccgtgat ttagccagcg
      601 cggtttttgg tacaggtggg cagataacaa cagttaatga taaaggcatc acaatcacaa
      661 caccggctgc gggcggtggt caggggaata cggtttccct taccggctcc ggcctgaata
      721 acggtggaaa tcagataacc agtgtggcca gtggtctgaa cggcactgcg cttgctgacg
      781 ccggtggaga tgttctgaat aatgcggcca atatcggtga cctgaaaaat gctctccgtg
      841 acagcagtgc ggcactg
//
LOCUS       DAZKHK010000420          857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKHK010000420 DAZKHK010000000
VERSION     DAZKHK010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.423"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>857
                     /locus_tag="ACHOS5_005526"
     CDS             <1..>857
                     /locus_tag="ACHOS5_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9780153.1"
                     /translation="SMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGDKG
                     LKISLNNDLTVGDKAPGSIKVNGADGKEAVSLNGQDGTIKLTSSPAAGGKSASATIAV
                     NAGKGGLTDAAGTTKTRLTYTPDRKSPAEELATMNDGLSFEGDDGKVIHKKLNEKLTI
                     KGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITI
                     TTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKN
                     ALRDSSAAL"
BASE COUNT          244 a          181 c          247 g          185 t
ORIGIN      
        1 ccagtatgca gggtgcaggt ttcgcactga aagactctga tgataaggaa gtgaaacagt
       61 ctcttgggca ggcgattaaa gtatccgggg acagcaatat caccaccaca gccatcacca
      121 atggagataa ggggttaaaa atatccctga ataatgacct gactgtgggg gataaggctc
      181 ccggcagcat caaagttaac ggggctgatg gtaaggaggc tgtgagtctg aatggtcagg
      241 atggcacgat taaactgaca tcctcgcctg ctgcgggcgg aaagtctgcg tcagcaacca
      301 ttgctgttaa tgcaggcaaa ggtggattaa cagatgctgc cggtaccacg aagacacgtc
      361 tgacttatac cccggataga aaatcaccgg cagaagagct ggcgaccatg aatgacggtc
      421 tttcttttga gggtgatgac ggaaaggtta ttcataaaaa actgaatgaa aaactgacca
      481 tcaaaggtgg tgctggtgat aatgaggatg taacagacag taacattcgt gttgatgcaa
      541 atgaaaaggg agatggtctt ctcgtccgga tgaccagaaa cctccgtgat ttggccagcg
      601 cggtttttgg tacaggtggg cagataacaa cagttaatga taaaggcatc acaatcacaa
      661 cacctgctgc gggcggtggt caggggaata cggtttccct taccggctcc ggcctgaata
      721 acggtggaaa tcagataacc agtgtggcca gtggtctgaa cggcactgcg cttgctgacg
      781 ccggtggaga tgttctgaat aatgcggcca atatcggtga cctgaaaaat gctctccgtg
      841 acagcagtgc ggcactg
//
LOCUS       DAZKHK010000421          857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKHK010000421 DAZKHK010000000
VERSION     DAZKHK010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.424"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(638..>857)
                     /locus_tag="ACHOS5_005527"
     CDS             complement(638..>857)
                     /locus_tag="ACHOS5_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PapB/FocB family fimbrial expression
                     transcriptional regulator"
                     /protein_id="HFP9780154.1"
                     /translation="SVNDEHFSLLIEISPIRSEKTIVALREYLVYGYSKQESCDRNNV
                     SNSYFCLCLKKLNYTHGIVSALKYFYQT"
BASE COUNT          264 a          165 c          180 g          248 t
ORIGIN      
        1 gcaaggtgtt cccaacggac gactcggtgc gaaaagtgat ttatctggcg atacagtcgg
       61 cgtcaaaaaa atggagtatg ccaatccaga actggcggct ggcaatgagc cgttttatta
      121 tcgagtttgg tgaccgcctt agcgagcacc tttaatacgg cggcagttac acagaattat
      181 ttacagggtc gactgggaac atattcaggc agtgtatctg aatccagaaa agaaacagca
      241 taatgtaatt ttattacaaa aggcgacaat tagcttgaaa acgccgttat tgggtgttct
      301 actaccatga aaatacgcat ttttcacacc gcacattacg aaactcgcat cacggagaat
      361 gaaagctatc agacactggt tgctattttt gaccacagaa ggtagtgctg tttaccgcca
      421 gagctgtgct ggcggggagc gaatccgaac catgtcgata attgtcaccg gccagagaac
      481 tatgtggcat caattttgct gaaaaatact gcagcaatca gaggactgac gcccgggatt
      541 tttaaacaaa ttcagttttc atcgtagtat cactttatca gccaagtcat aaagttcagc
      601 tgtattttgg gaggtaaagc gatattatga ttatatttta tgtttgatag aaatatttca
      661 gggcagatac aataccatgg gtataattca gttttttcag acacaaacaa aaataactat
      721 tggatacatt atttctgtca cagctttctt gttttgagta accataaacc aaatattctc
      781 taagagccac tattgttttt tcacttctga ttggtgatat ttcaatcaga agcgaaaagt
      841 gctcatcatt tacactt
//
LOCUS       DAZKHK010000422          857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKHK010000422 DAZKHK010000000
VERSION     DAZKHK010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.425"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..629)
                     /locus_tag="ACHOS5_005528"
     CDS             complement(<1..629)
                     /locus_tag="ACHOS5_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9780155.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDP"
     gene            complement(626..>857)
                     /locus_tag="ACHOS5_005529"
     CDS             complement(626..>857)
                     /locus_tag="ACHOS5_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780156.1"
                     /translation="KDAIDEASRERTEIPASFMSIRRATTKSGNALTFVAERSDVTGH
                     ADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
BASE COUNT          177 a          246 c          245 g          189 t
ORIGIN      
        1 gggtcgttcg tatacagcaa aaaccccata tgcgccccgt tgttgtagta acggcgacga
       61 aaaaggctgg cctcgctgtt cagcagcgcc gagtgaatgc caccgatata atccggcagc
      121 ccgtaaattt gctgacgcgg atcatacatc cgcagaaaga taatatcttc cggcgtatag
      181 accagcgcgg ggccttcctg aagcaccacg aactcaccgg ttttgcggcg ccggagatac
      241 agcgacggaa gcggctcaag tgccacgacg tcgccgaata cgttacgcac cttgagcagg
      301 gcgagatcgc cgaacaacag gtagtcaaat accgcctgtt ccatctggtc cgttgtgaga
      361 ccaccaccgg tatagccacc cgccaccatg ttacggcggg catacagcac cccaccgtgc
      421 tgggcgttaa ggttcgggag ctgcgccagt gccaggcggt taatcggcag ctgccagtgg
      481 ttgtgttcgt tgtcatacca gatgttatgg tagtccgtgc cggtggtcag caccggttcc
      541 ggctggccaa aggtgattgt gctgccacgt gcgggcgtga atgctttttt cgtcacgcgt
      601 gacgttttgt ttctgcgttt cttgctcatg cggctttccc gaaggcccat gtggacgggc
      661 gttcatagtc ataatctaca ggctcattaa tcacggcgtg cgaaatggca aaaaacacat
      721 ccgcatgccc cgtaacatcc gaccgttccg ccacgaacgt cagcgcgtta ccgctttttg
      781 tggtggcgcg gcgtatggac atgaacgacg cggggatctc cgtgcgttca cggctggcct
      841 catcaatggc gtctttc
//
LOCUS       DAZKHK010000423          857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKHK010000423 DAZKHK010000000
VERSION     DAZKHK010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.426"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..629)
                     /locus_tag="ACHOS5_005530"
     CDS             complement(<1..629)
                     /locus_tag="ACHOS5_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9780157.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDP"
     gene            complement(626..>857)
                     /locus_tag="ACHOS5_005531"
     CDS             complement(626..>857)
                     /locus_tag="ACHOS5_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780158.1"
                     /translation="KDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDVTGH
                     ADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
BASE COUNT          176 a          246 c          243 g          192 t
ORIGIN      
        1 gggtcgttcg tatacagcaa aaagcccata tgcgccccgt tgttgtagta acggcgacga
       61 aaaaggctgg cctcgctgtt cagcagcgcc gagtgaatgc caccgatata atccggcagc
      121 ccgtaaattt gctgacgcgg atcatacatc cgcagaaaga taatatcttc cggcgtatag
      181 accagcgcgg ggccttcctg aagcaccacg aactctcctg ttttgcggcg ccggagatac
      241 agcgacggaa gcggctcaag tgccacgacg tcgccgaata cgttacgcac cttgagcagg
      301 gcgagatcgc cgaacaacag gtagtcaaat accgcctgtt ccatctggtc cgttgtgagg
      361 ccaccaccgg tatagccccc cgccaccatg ttacggcggg catacagcac cccaccgtgc
      421 tgggcgttaa ggttcgggag ctgcgccagt gccaggcggt taatcggcag ctgccagtgg
      481 ttgtgttcgt tgtcatacca gatgttatga tagtccgtgc cggtggtcag caccggttcc
      541 ggctgaccaa acgtgattgt gctgccacgt gcgggcgtga atgctttttt cgtcacgcgt
      601 gacgttttgt ttctgcgttt cttgctcatg cggctttccc gaaggcccat gtggacgggc
      661 gttcatagtc ataatctaca ggctcattaa tcacggcgtg cgaaatggca aaaaacacat
      721 ccgcatgccc cgtaacatcc gaccgttccg ccacgaacgt cagcgcgtta ccgctttttg
      781 tggtggtgcg gcgtatggac atgaacgacg cggggatctc cgtgcgttca cggctggcct
      841 catcaatggc gtctttc
//
LOCUS       DAZKHK010000424          856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKHK010000424 DAZKHK010000000
VERSION     DAZKHK010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.427"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            117..833
                     /locus_tag="ACHOS5_005532"
     CDS             117..833
                     /locus_tag="ACHOS5_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9780159.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          289 a          142 c          174 g          251 t
ORIGIN      
        1 tagaatattt ttatttctaa tagacgagat ttttcctgtt atgatataat atgccgaatt
       61 aacacatgtt aacgatttac cagtaatgta aataaatttt cgaggagatc attccagtgg
      121 gacgtaaatg ggccaatatt gttgctaaaa aaacggctaa agacggtgca acgtctaaaa
      181 tttatgcaaa attcggtgta gaaatctatg ctgctgccaa acaaggtgaa cccgatccag
      241 aattaaacac atctttaaaa ttcgttattg aacgtgcaaa acaagcacaa gttccaaagc
      301 acgttattga taaagcaatt gataaagcca aaggcggcgg agatgaaacg ttcgtgcagg
      361 gacgttatga aggctttggt cctaatggct caatgattat cgctgagaca ttaacttcaa
      421 atgttaaccg tacgattgct aacgttcgca caattttcaa taaaaaaggc ggcaatatcg
      481 gagcggcagg ttctgtcagc tatatgtttg acaatacggg tgtgattgta tttaaaggaa
      541 cagaccctga ccatattttt gaaattttac ttgaagctga agttgatgtt cgtgatgtaa
      601 ctgaagaaga aggtaatatt gttatttata ctgaacctac tgaccttcat aaaggaatcg
      661 cggctctaaa agcagctgga atcactgagt tctcaacaac agaattagaa atgattgctc
      721 aatctgaagt tgagctttcc ccagaagatt tagaaatctt tgaagggctt gttgatgccc
      781 ttgaagatga cgacgatgtt caaaaagttt atcataacgt cgcaaatctc taattatatg
      841 ttaaagaaat ctgtct
//
LOCUS       DAZKHK010000425          849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKHK010000425 DAZKHK010000000
VERSION     DAZKHK010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.428"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..>849
                     /locus_tag="ACHOS5_005533"
     CDS             80..>849
                     /locus_tag="ACHOS5_005533"
                     /inference="COORDINATES: protein motif:HMM:NF017476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9780160.1"
                     /translation="MAIGADSVDKAATPVSGATIKLKNGGTIEYKGFAGDKAASVVSV
                     GDAGKERQIVNVGAGAISDTSTDAINGSQLYAIAKTLKDDLDAINPTWKLKDSTGKEK
                     EVGSKNGGTTGNEVTVIGKGDIDVTVDGNGLSIDGTKLAENIKKDAIKIKTGDGQTLT
                     KKLGEEVEFNDAGPIKTKINNGKLEITAETASFTPDGNGGNGKITVSNGDDDKLVTAK
                     KIADAINQSGWKIKADSENGGKVEGDNKEELINPGDTVT"
BASE COUNT          284 a          137 c          246 g          182 t
ORIGIN      
        1 agggagtcag agtacgacgg gcaaaaagtc cacggttgct ctggggcagg gagctacagc
       61 ctctgccgcg gaggggaacg tggccatcgg tgctgattca gtggacaagg cagcaactcc
      121 ggtttccggc gctaccatta agttaaagaa tggcgggact attgaataca agggctttgc
      181 cggagataag gctgcatcag tagtatctgt tggtgatgcg ggtaaagagc gtcagattgt
      241 taacgtgggg gcgggtgcga tttctgacac aagtacagat gccattaacg gtagtcagtt
      301 atatgcgata gcgaagacac tgaaagatga ccttgatgcc attaatccga cgtggaagtt
      361 aaaagacagc acaggaaaag aaaaagaggt cggcagtaaa aatggcggga ctacaggtaa
      421 tgaagtgacc gtcatcggta aaggggatat tgatgttact gttgatggaa atgggctgag
      481 tattgatggc acaaagcttg ctgaaaacat caaaaaagat gccatcaaga ttaaaacggg
      541 agatggtcaa acactaacga aaaaacttgg tgaagaagtt gagtttaatg atgccggacc
      601 aataaaaaca aaaattaata atggcaaact tgagataact gcagaaacag ccagttttac
      661 gcctgatgga aatggtggta acgggaagat tacagtaagc aatggtgatg acgataagct
      721 tgttactgcg aaaaagattg ctgatgccat aaaccagtct ggctggaaaa ttaaagctga
      781 cagtgaaaat ggtgggaaag ttgaggggga taataaagag gagctaatta atcccggtga
      841 tacagttac
//
LOCUS       DAZKHK010000426          842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKHK010000426 DAZKHK010000000
VERSION     DAZKHK010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.429"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..354)
                     /locus_tag="ACHOS5_005534"
     CDS             complement(<1..354)
                     /locus_tag="ACHOS5_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9780161.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCMNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGV"
     gene            complement(365..>842)
                     /locus_tag="ACHOS5_005535"
     CDS             complement(365..>842)
                     /locus_tag="ACHOS5_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9780162.1"
                     /translation="ARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRRVYARFLDAVNF
                     VAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTETDGALFPGRIMLANTCMWD
                     YRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMAVNFGGFLSINKLSQ"
BASE COUNT          178 a          295 c          220 g          149 t
ORIGIN      
        1 cacaccgtgt tcaaagcgcc gtccggtcag gtgcggcaca cagcggaacc ggtgaatttc
       61 accccggcag accagccacc agggcagggc actctttatc tgcagcctcc ggtccgcctc
      121 gctcagccag ggcagaccac cgggatgact gtggaccagc gccacaatct ccccctgcat
      181 ctgtgcctgc agccagtctt ccggtgcaat acgaaaatac gcctccggct ctgcggagat
      241 attcatgcag ggctggtacc gttcgccctc cggggtgctt atcacgaagc cgcacgactc
      301 cgctggcgca caccgccggg catgcaccag aatcgctgat tcagtctgtg tcataaaacg
      361 ggatttactg cgaaagttta ttgatggaaa ggaaaccgcc aaaattgacc gccatgccgc
      421 gcatctcaca cccgcgcatg catttactgc atctgtcctt ccggatatcc gtggtggggt
      481 tgtcgaactc atccgccact gccggaccgt tatacccgca ctcatcgccc cggtaatccc
      541 acatacaggt gttcgccagc atgatgcgac cgggaaacag cgccccgtcc gtctccgtcg
      601 gtgtcgccag cacaaacgag gccgtcatgg ccgtcagcga tgacatctgc tccaccaccc
      661 accggtccgt cagctcctgc tccgggtcgg cctcaggatt gcctgccaca aaattcaccg
      721 catccagaaa acgcgcatac acccggcgac ggaccaccgt ggcacccacc aggctctgca
      781 aatcctccgc catcccggtg acaaggccga aaagattgga gaccgtcagc gacgggcggg
      841 ca
//
LOCUS       DAZKHK010000427          842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKHK010000427 DAZKHK010000000
VERSION     DAZKHK010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.430"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..732)
                     /locus_tag="ACHOS5_005536"
     CDS             complement(<1..732)
                     /locus_tag="ACHOS5_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP9780163.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRT"
BASE COUNT          234 a          211 c          194 g          203 t
ORIGIN      
        1 tgtccgacga tcggtatgtt ccggtgcaga ctctggaggt tcagagttcc atgtaacgtt
       61 gtaagccacc ttcagtccaa aatgttcatt aatagcaaca tttaatgcgc tttcggagtt
      121 cagcgttgtg tcttccgcgc caaagaccga aacaccctgc gtaaatttag cgttgtcagt
      181 caactgccat gcataagccc cggaggcata gcccagcggc tgggtttcac tggcattgtc
      241 ggtatatttg tcgtaacgca caccagggcc gaattcaaag cggaaactgt gtaccgggcc
      301 attcaaaaac tggcgaccat aacccgcggt caacacatcg cgctctcgat agccgttata
      361 acggtctgtc agccagcttg cctgaccaaa taaatagtca taatcagtta aattaaaacg
      421 gctacgcccg cccgccgcat atttttctga agaacgctca tcattagaag aagtattact
      481 ggcgttcccc cacagtgacc aggcagtggt ttgtccatac caggtcatag tagtgtcagc
      541 cgtaagtgag gagcttttcg tattgcctga ttgtgcaaga tatcctgcgt tcagattacc
      601 ttcgaaaggt tttttagcgc tggcaggatc gtccatgaca gtaaaaacgg aatcatcggc
      661 agctgcattc agtgacgcaa acatgccccc cgccaacata acgatggcag gaactgtctt
      721 caaaagcttc atttatcaag agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa
      781 actttcttaa ggatcaaaga ttagcgtccc tggaaaggta acgaattata aaaaggcgcg
      841 aa
//
LOCUS       DAZKHK010000428          841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKHK010000428 DAZKHK010000000
VERSION     DAZKHK010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.431"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(73..>841)
                     /locus_tag="ACHOS5_005537"
     CDS             complement(73..>841)
                     /locus_tag="ACHOS5_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780164.1"
                     /translation="NDKIEPGKGANRVDGGSGYDTLLLSDVMDDWNIYRVSDGTLFFH
                     SERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDHRYLLKWRNKNVDYGKHTEG
                     TNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGASGNDELYGAEGNDW
                     LYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKGEKDFLFLSKGIVTSTDEFI
                     SCLKQTGNDVEAGIAGGQIYFVNTALEDINHNSVYIV"
BASE COUNT          272 a          229 c          104 g          236 t
ORIGIN      
        1 gcacacaaaa acaaaacaca caatgcattg attttaaaca aaaaaacaaa acagaaactc
       61 aacacataaa aatcagacaa tataaacaga attgtgattt atatcctcaa gcgctgtatt
      121 aacgaaatat atctgtcctc ctgcaatacc tgcctcaaca tcatttccgg tttgtttcag
      181 acatgaaata aactcatctg tcgatgtaac aatacccttt gataaaaaca gaaaatcttt
      241 ttcaccctta tgtttattaa aatccatgat aaatgaattc ccttcacaag cagcatcaaa
      301 gacgaagaca tcatgaccag taccaccata cagataatca ttacctcccc caccatataa
      361 ccagtcgtta ccttctgctc cgtacaactc atcattacca gaagccccat gaagataatc
      421 attcccgcga cctccgtgta aaagacttac tccttctccc ccggcactat gtaatatatc
      481 atttccgtca agaccaaaaa taatatttcc gaacagacta tcatctccgt ttgtaccttc
      541 tgtatgtttt ccgtaatcaa catttttatt acgccacttc agaagatacc tgtggtctgt
      601 aatctgctct tcttctatct gataagggct tagtttactc ctccagtcac cagaaaaaga
      661 gataccttca atacctgttg cctcagtcag accggctccc cgttctgaat ggaaaaacag
      721 cgtcccatca ctgaccctgt atatattcca gtcatccatt acatcgctaa gaagcagcgt
      781 gtcataccca ctgccaccat caaccctgtt tgctccctta cctggttcaa tcttatcatt
      841 c
//
LOCUS       DAZKHK010000429          838 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKHK010000429 DAZKHK010000000
VERSION     DAZKHK010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.432"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>838
                     /locus_tag="ACHOS5_005538"
     CDS             <1..>838
                     /locus_tag="ACHOS5_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9780165.1"
                     /translation="KLKEGWQPYGSALISTAGYGAEFIQPVVSEGSISSPEEPGNRPT
                     TSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAAC
                     IYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGCVGQGLHIAKKLLPFIPANAGILL
                     VPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGRPLYKDLIGRTKAALAKNPKNVLL
                     AVVWMQGEFDFNGTPANHAARFTEVVDQYRADLADMAGQCVGGSADSVPWICGDTTYF
                     WKQ"
BASE COUNT          195 a          196 c          249 g          198 t
ORIGIN      
        1 aaaactgaag gaagggtggc agccttatgg tagtgcgctg atttcgacag ctggttatgg
       61 tgcggagttc atccagccag ttgtgagtga ggggagcatc tcatcaccag aggagccagg
      121 caaccgtccg acgacctcag cgccttctgt tgcgccagaa tattactatg tgatcgcgct
      181 tgctggtcag tccaatggta tgtcatacgg tgagggactg ccattaccgg atacattcga
      241 cagccctgat ccacgtatta aacagttagc gcgtcgcagt acggtgacac cgggaggtgc
      301 tgcatgtatc tataacgata ttatcccggc agatcactgt ctccatgatg tggaggatat
      361 gacagggctt aatcatccca aagcggattt atcgaaaggt cagtacggct gtgtgggaca
      421 ggggctgcat atcgccaaaa agcttctgcc atttataccg gcgaatgcgg gcattcttct
      481 tgttccgtgc tgccgtggtg gttcagcttt caccaccgga accgatggca catacagtga
      541 cactactggc gcctcagaga gttccacccg ctggggggtg ggcaggccgc tgtataagga
      601 cctcattggt cgtacaaaag ccgcgctggc gaagaatccg aaaaatgtgc tgcttgccgt
      661 ggtatggatg caaggggaat ttgattttaa cggaacgcca gcgaatcatg cagcccgttt
      721 tacagaagtg gtggatcaat accgtgcgga ccttgcagat atggcggggc agtgtgtggg
      781 tggctcagct gacagtgttc cctggatttg tggagacaca acatatttct ggaagcag
//
LOCUS       DAZKHK010000430          834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKHK010000430 DAZKHK010000000
VERSION     DAZKHK010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.433"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..561
                     /locus_tag="ACHOS5_005539"
     CDS             <1..561
                     /locus_tag="ACHOS5_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780166.1"
                     /translation="QKILRPAEDNFMEKVDDLNSEQQYDNIAGTTNLGYQKQFGEARK
                     ELAGNLAQSGVDPSSGRFNAVMNANQSDQVTGQIDTTTRGQVSQADKYVAGLQDVAAL
                     GSGQKADALQSFNSLADSSLAKAKSDAQAAFTKQQGRASLVGAGLGAAGAYAMHKAGG
                     SGGSGGAKTPGTGANAIQHQAQNWRL"
     gene            564..>834
                     /locus_tag="ACHOS5_005540"
     CDS             564..>834
                     /locus_tag="ACHOS5_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780167.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGV"
BASE COUNT          213 a          200 c          260 g          161 t
ORIGIN      
        1 caaaaaatcc tccgcccggc tgaagataac ttcatggaaa aggtcgatga tctgaacagt
       61 gagcagcagt acgacaatat tgctggcacc acaaatctgg ggtatcagaa acagtttggc
      121 gaagcacgga aggagctggc gggtaatctt gctcagtccg gtgttgaccc gtccagtggt
      181 cgttttaacg cggtaatgaa tgcgaaccag agtgatcagg taaccgggca gattgacaca
      241 accacacggg ggcaggtatc gcaggcagat aagtatgttg ccgggctaca ggatgttgca
      301 gctctcggtt ctggtcagaa ggcggatgcg ttacagagtt ttaactcgct ggcagacagc
      361 agtctggcaa aagctaaatc ggacgcacag gctgcgttta cgaaacagca ggggcgagcc
      421 tctcttgttg gcgctggttt gggtgcggca ggtgcatatg cgatgcataa ggctggcggt
      481 agcggaggaa gtggcggtgc taaaacacct ggcaccggcg ctaatgccat tcagcatcag
      541 gctcagaact ggagactgtg attatggagt acggtaaata cgaaacactt gcgagatacg
      601 gttataccgg agctgcccgt ccacagggtg actggcagac gtcagcagcg ctgacacgcc
      661 aacaatacga cgactggcga accagatatt tgccccgcgt ggcaaggctg gctgaccttg
      721 gcgagaacaa cagcctgatg aatgcacagc ttgcccgggt gggcggcctt gccacttcca
      781 gtctccgtac agcgcagatg gcgcaggata accagatggc gagatacggg gtaa
//
LOCUS       DAZKHK010000431          821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKHK010000431 DAZKHK010000000
VERSION     DAZKHK010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.434"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>821
                     /locus_tag="ACHOS5_005541"
     CDS             <1..>821
                     /locus_tag="ACHOS5_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9780168.1"
                     /translation="EGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAF
                     GQSSTEIIRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARF
                     RSEFSAMGAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAI
                     LGTLSRTMGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRN
                     IEGNVAAQQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMT"
BASE COUNT          174 a          219 c          283 g          145 t
ORIGIN      
        1 gaaggtatcc gcgccgcgat ggatccggcg tgggagatgc agaaggcgct gggcgagctt
       61 gaggccaaag gcgtcggggc gcaggcgctg accgccctca ccacgcaggc catgaaactg
      121 gggaccgcat tcggtcagtc ctccacggag atcatccgtt ctgcatggca tattcgcggc
      181 gcactggacg gactgagcga cggggatttg ccccgtgtga cggcagcaac cagcaccctg
      241 gccgtggctg cccgcgtcag cagcgaagag gcgagcggct accttgccgc catgggtgcc
      301 cgtttccgca gtgagttttc tgccatgggt gccgtggggt ttgctgaaga tctggcaggt
      361 aaaaccgcgt ggatggttca gcagttcggc gtggaaatga gcaccatgca ggagcttatc
      421 gagggcacca aaaacgcggg cgcggatttt ggcgtcagca tggcagagca gtttgccata
      481 ctgggcaccc tgtcacggac catgggtacc gaggcatcag gcatctatga ggagttcctg
      541 cgcagtgccc ccgctgccgc acaaaaaatg gggatgagtt ttgtcgatgc caccgggaag
      601 attttaccca tgggtgacat cctcgaccgt ctgcaggcga ggttcggcag gaacattgag
      661 ggtaacgtgg cggcacaaca ggcgctggat gacgccttcg gcagtggcgc ggacgtgatt
      721 aaaaaactct ggggacagca ggaaatgctt aaccggagca tatcagcgct ggggcgtaat
      781 gacggcatga agcgtgccac ggaaatggcg gaagccatga c
//
LOCUS       DAZKHK010000432          821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKHK010000432 DAZKHK010000000
VERSION     DAZKHK010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.435"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>821
                     /locus_tag="ACHOS5_005542"
     CDS             <1..>821
                     /locus_tag="ACHOS5_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9780169.1"
                     /translation="EGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAF
                     GQSSTEIIRSAWHIRGALDGLSDGDLPRVTAATSTLAMAARVSSEEASGYLAAMGARF
                     RSEFSAMGAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAI
                     LGTLSRTMGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRN
                     IEGNVAAQQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMT"
BASE COUNT          176 a          219 c          281 g          145 t
ORIGIN      
        1 gaaggtatcc gcgccgcgat ggatccggcg tgggagatgc agaaggcgct gggcgagctt
       61 gaggccaaag gcgtcggggc gcaggcgctg accgccctca ccacgcaggc catgaaactg
      121 gggaccgcat tcggtcagtc ttccacggag atcatccgtt ccgcatggca tattcgcggc
      181 gcactggacg gactgagcga cggggattta ccccgtgtga cggcagcaac cagcaccctg
      241 gccatggctg cccgcgtcag cagcgaagag gcgagcggct accttgccgc catgggtgcc
      301 cgtttccgca gtgagttttc tgccatgggt gccgtggggt ttgctgaaga tctggcaggt
      361 aaaaccgcgt ggatggttca gcagttcggc gtggaaatga gcaccatgca ggagcttatt
      421 gagggcacca aaaacgcggg cgcggatttt ggcgtcagca tggcagagca gtttgccata
      481 ctgggcaccc tgtcacggac catgggcacc gaggcatcgg gcatctatga ggaattcctg
      541 cgcagtgccc ccgctgccgc acaaaaaatg gggatgagtt ttgttgatgc caccgggaag
      601 attttaccca tgggtgacat cctcgaccgt ctgcaggcga ggttcggcag gaacatcgag
      661 ggtaacgtgg cggcacaaca ggcgctggat gacgccttcg gcagtggcgc ggacgtgatt
      721 aaaaaactct ggggacagca ggaaatgctt aaccggagca tatcagcgct ggggcgtaat
      781 gacggcatga agcgtgccac ggaaatggcg gaagccatga c
//
LOCUS       DAZKHK010000433          820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKHK010000433 DAZKHK010000000
VERSION     DAZKHK010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.436"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..431)
                     /locus_tag="ACHOS5_005543"
     CDS             complement(<1..431)
                     /locus_tag="ACHOS5_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9780170.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHEVLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFS"
     gene            complement(428..>820)
                     /locus_tag="ACHOS5_005544"
     CDS             complement(428..>820)
                     /locus_tag="ACHOS5_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9780171.1"
                     /translation="VTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEATG
                     LYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRT
                     HSLWRHRAWRASAFTGIYNDLAAASTFV"
BASE COUNT          178 a          258 c          224 g          160 t
ORIGIN      
        1 gagaaatagg tgttctgctt accgttatcc gttgtctgtg tacggggagt tctggctttc
       61 ggtgccagca tctgcgccac accaccgagc accatactgg caccgagaga aaacaggatg
      121 ccggtcatac caccggcccc aatggctgcc ccccatgctg caagggtggc tccggcggta
      181 aagaatgatc cggcaatggc ggcagccccc aggacaatct ggaatacgcc acctgacttg
      241 gccccggcga ctctgggaac aatatgaatc acagcgccgt caggcagaac ctcatgtaac
      301 tgcgccgtca atccggacgt gctgacgtcc cgcccggcaa tccgtacctg ataccagccg
      361 tcgctcagtt tctgacgaaa cgccgggagc tgtgtggcca gtgcccggat ggcttcagcc
      421 cccgttttca cacgaaggtc gatgcggcgg ccaaatcgtt gtaaatcccc gtaaaggcag
      481 atgcgcgcca tgcccggtga cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtgt
      541 acctctctcg tttgctcagt tgttcaggaa tatggtgcag cagctcgccg tcgccgcagt
      601 aaattgcggc gtgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggct
      661 gtgccgctga caacggcacc tgatacagcc ccgtcgcctc cagattatcc agatagagat
      721 tctggccgtt acgccaccag tcatcctcac gatgaaagtc cggcatctca atccccgcca
      781 gatgataagc atcccggaac agtgtgtaac agtccgtcac
//
LOCUS       DAZKHK010000434          799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKHK010000434 DAZKHK010000000
VERSION     DAZKHK010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.437"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..242
                     /locus_tag="ACHOS5_005545"
     CDS             <1..242
                     /locus_tag="ACHOS5_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9780172.1"
                     /translation="PEEGANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDSTSHI
                     EAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            258..545
                     /locus_tag="ACHOS5_005546"
     CDS             258..545
                     /locus_tag="ACHOS5_005546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9780173.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSISADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            558..>799
                     /locus_tag="ACHOS5_005547"
     CDS             558..>799
                     /locus_tag="ACHOS5_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9780174.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGASLVVSSLGDFANIFQHAFGIQGVVP"
BASE COUNT          209 a          168 c          212 g          210 t
ORIGIN      
        1 gcccggaaga gggggctaac aagctgtcat tagcactgac cctgatccct tcaggtgtga
       61 attttgatgc cgatgaaaac gatccagtga aattactgat tgtgctggct gcaaccgaca
      121 gtaccagcca cattgaagcg atttctcaac tcgccaagtt attcgataac gaaaaagaca
      181 tacaggctat tttgacggcc aaaacgacgc aggacatttt gtctgttatt gcgcgctatt
      241 aatcctgagg aacatttatg aaaataacag tggtttgcgg taacggctta ggcaccagcc
      301 tgatgatgga aatgagcatt aaaagcattt tgaaggattt gtccatcagc gctgatgttg
      361 atcatgtcga cctgggctcc gcgaaaggga cgccgagtga tatctatgtc ggcaccaaag
      421 atattgccga gcagttagtc gcgcagtctg tcgcaggcaa aatcgtcgcc ctggaaaata
      481 tgattgataa gaaagccatg agagaacgtt tgtccgtggc attaaccgaa ttaggcgcac
      541 tgtaagctgg gggcgatatg gatttctttc gttttttgat gagcgatgta ctttccgaac
      601 ccgcggtgct ggtcggttta atcgccctga ttggcctgat tgcacagaaa aaaccggtaa
      661 ccgaatgcat taaaggtacc gtcaagacca ttatggggtt tgtgatttta ggcgcagggg
      721 cgagtttagt tgtgtcgtca ctgggtgact tcgcaaacat tttccagcat gcctttggta
      781 ttcagggtgt cgtgccgaa
//
LOCUS       DAZKHK010000435          796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKHK010000435 DAZKHK010000000
VERSION     DAZKHK010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.438"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..506)
                     /locus_tag="ACHOS5_005548"
     CDS             complement(<1..506)
                     /locus_tag="ACHOS5_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9780175.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPSFRQKLNEGWYQVRISGRDAGENELSARLNEPLANGAVIHVVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPVGWLGAAAVSGLYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFS"
     gene            complement(404..>796)
                     /locus_tag="ACHOS5_005549"
     CDS             complement(404..>796)
                     /locus_tag="ACHOS5_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9780176.1"
                     /translation="VTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEATG
                     LYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRT
                     HSLWRHRAWHASAFTGIYNDLAAASTFV"
BASE COUNT          177 a          262 c          203 g          154 t
ORIGIN      
        1 gagaaatacg tgttctgttt gccgttatct gtactggctg ccgtgggcgt cctggctttc
       61 ggtgccagca tctgcgccac accacccagg atcatactgg cccctgctgc atacagaccc
      121 gatacagccg cggcccccag ccagccaaca gggttccacc atgccaccgc aatcagcgcc
      181 gcacccagca ccgcctgaaa cacaccgcca cttttggcac ccgccagacg cggaacgacg
      241 tggatcacgg caccatttgc cagcggttca ttaagacggg cagataattc attttcgcct
      301 gcatcacgcc cggaaatgcg cacctgatac cagccctcat tcagtttctg gcgaaacgac
      361 ggaagctgtg tggccagcgc ccggatggct tcagcccccg ttttcacacg aaggtcgatg
      421 cggcggccaa atcgttgtaa atccccgtaa aggcagatgc gtgccatgcc cggtgacgcc
      481 agagggagtg tgtgcgtcgc tgccatttgt cggtatacct ctctcgtttg ctcagttgtt
      541 caggaatatg gtgcagcagc tcgccgtcac cacagtaaat ggcggcatga ttcggcaccg
      601 atgaaccaaa acagcacagc agcacatcgc ccggctgcgc cgctgacaac ggcacctgat
      661 acagccctgt ggcctccaga ttatccagat agagattctg accgtgacgc caccagtcat
      721 cctcgcgatg aaaatccggc atctcaatcc ccgccagatg ataagcatcc cggaacagcg
      781 tgtaacagtc cgtcac
//
LOCUS       DAZKHK010000436          793 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKHK010000436 DAZKHK010000000
VERSION     DAZKHK010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.439"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            360..>793
                     /locus_tag="ACHOS5_005550"
     CDS             360..>793
                     /locus_tag="ACHOS5_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9780177.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVTTPVVVTGDDGKEVLISATSEPEYYYVITLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQ"
BASE COUNT          182 a          203 c          222 g          186 t
ORIGIN      
        1 gtggttggtt atatccctta tcctttctgt gcgggttcag gtgctgatac tgaactcacc
       61 gggaggcacc cggcaccatg tatatgatta acagatatgt agcgtcagcc cctctccgga
      121 ggggctttct tatggataaa aaaagcccgc gccgggagac gcgggcagca aggaataaac
      181 aacaaaacgt gaagtaatat ttcagctggc gaataatacc cgacagtaat cactctgcgc
      241 aactgcgtgg cctttttcgt attgcgggct gtagtcttcc tcctgtcatt gtcctgtaac
      301 ttccggactt cagcccgctc cttatccgac taacaacatt atcccgaccg ggaggattca
      361 tgacatttaa acactacgat gtggtcaggg cggcatcgcc gtcagacctt gcggatgcgc
      421 ttgctcaaaa aattcgtgaa ggatggcagc catacggcgg gccgttttct tcgtatacgg
      481 atgatggcgc agcacttatt caggcgattg tcgctgaagg tgatgtcacc acgccggtgg
      541 tggtaaccgg tgatgatggt aaggaggtgc ttatcagcgc caccagcgaa ccggagtatt
      601 actacgtgat tacgctggct ggccagtcaa acagcatggc atatggtgaa ggccttccgc
      661 tgccggagac gtatgaccgt ccggacccgc gtattaagca gctggcgcgc cgcagtacgg
      721 tgacaccggg aggtgccgcc tgtaagtata acgacattat tccggcggac cactgtctgc
      781 atgatgtgca gga
//
LOCUS       DAZKHK010000437          787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKHK010000437 DAZKHK010000000
VERSION     DAZKHK010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.440"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>787
                     /gene="istA"
                     /locus_tag="ACHOS5_005551"
     CDS             <1..>787
                     /gene="istA"
                     /locus_tag="ACHOS5_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFP9780178.1"
                     /translation="AVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMT
                     ILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYI
                     EFTDNMRYDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQF
                     GKEMGFSPRLCRPFRAQTKGKVERMVQYTRSSFYIPLMTRLRPMGITVDVETANRHGL
                     RWLHDVANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHL"
BASE COUNT          178 a          214 c          211 g          184 t
ORIGIN      
        1 gctgttgctt cactcctgga tgaataccgg gattatattc gtcaacgcat cgccgatgct
       61 catccttaca aaatcccggc aacggtaatc gctcgcgaga tcagagacca gggatatcgt
      121 ggcggaatga ccattctcag ggcattcatt cgttctctct cggttcctca ggagcaggag
      181 cctgccgttc ggttcgaaac tgaacccgga cgacagatgc aggttgactg gggcactatg
      241 cgtaatggtc gctcaccgct tcacgtgttc gttgctgttc tcggatacag ccgaatgctg
      301 tacatcgaat tcactgacaa tatgcgttat gacacgctgg agacctgcca tcgtaatgcg
      361 ttccgcttct ttggtggtgt gccgcgcgaa gtgttgtatg acaatatgaa aactgtggtt
      421 ctgcaacgtg acgcatatca gaccggtcag caccggttcc atccttcgct gtggcagttc
      481 ggcaaggaga tgggcttctc tccccgactg tgtcgcccct tcagggcaca gactaaaggt
      541 aaggtggaac ggatggtgca gtacacccgt agcagttttt acatcccact aatgactcgc
      601 ctgcgcccga tggggatcac tgtcgatgtt gaaacagcca accgccacgg tctgcgctgg
      661 ctgcacgatg tcgctaacca acgaaagcat gaaacaatcc aggcacgtcc ctgcgatcga
      721 tggctcgaag agcagcagtc catgctggca ctgcctccgg agaaaaaaga gtatgacgtg
      781 catcttg
//
LOCUS       DAZKHK010000438          776 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKHK010000438 DAZKHK010000000
VERSION     DAZKHK010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.441"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(33..776)
                     /locus_tag="ACHOS5_005552"
                     /pseudo
     CDS             complement(33..776)
                     /locus_tag="ACHOS5_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          210 a          174 c          137 g          255 t
ORIGIN      
        1 caggttgttt ctatccatag ctgccctttc tattcaaacg tgaaatcaat aagctcatca
       61 gggacatcac aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactcatt
      121 gcgtaactgt catgctctgg attatttgat tctttcagcg gaaatttttc ctcgaaggct
      181 ttatttagcc gcccctggtt tccttttaca gcgaataaat aatcacctcc ctgtttttgt
      241 atcttctctg caatatcttt ctggcaaccc atcgcatcgg ttgtgatgat ttttccttta
      301 atatccagca tgttaagaag ttcagggata gctgtgatct cattggattt cttatccgtc
      361 ttgatctgtc cgatgaccag actgtgcatt gttgagaacg cactaatgat atgaatcgct
      421 cccttacggc gactcttgtc ataagaatgc cggtgtattt ttccatcaat tgcgatgacg
      481 tctttatcat ctgaagaatg gtagtcaagc atccagttaa taaagctctc atgaaatttc
      541 gcaggacaga tacaggatac aactctggca atggtatcgt gaacaggaat accattttca
      601 aaatcaccat attgcttcaa aaaatcggga tgtgtttccc caaaatcctc tatatcttcc
      661 cagccttctg caccagaatc gacagcgtaa atagtcaaca gtagaatatc cgacaattta
      721 tgtttcactt tccaggtttg tctgtaatcg ggaataatag aaatatgttc catcaa
//
LOCUS       DAZKHK010000439          770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKHK010000439 DAZKHK010000000
VERSION     DAZKHK010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.442"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>770
                     /locus_tag="ACHOS5_005553"
     rRNA            <1..>770
                     /locus_tag="ACHOS5_005553"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          217 a          167 c          236 g          150 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcctcttaat ggggtgactg
      181 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      241 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      301 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      361 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      421 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      481 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
      541 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
      601 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
      661 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
      721 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg
//
LOCUS       DAZKHK010000440          758 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKHK010000440 DAZKHK010000000
VERSION     DAZKHK010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.443"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..516
                     /locus_tag="ACHOS5_005554"
     CDS             <1..516
                     /locus_tag="ACHOS5_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9780179.1"
                     /translation="GDAWIPPHTGLPAHSTDIAPPDIPEGHVAIFDEDENAWHLMEDH
                     RGKAVWRLESGSALLITEPGPLPENVTEIKPDGEFCRWDGSSWVKDEAAEQAYRVSEA
                     ENKKRELIQEATNTINILQDAVDLNMATDDESALLPLWKKYRVLLNRTDTGNAPDIVW
                     PESPSPDHHAG"
     gene            528..>758
                     /locus_tag="ACHOS5_005555"
     CDS             528..>758
                     /locus_tag="ACHOS5_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780180.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFI"
BASE COUNT          202 a          202 c          214 g          140 t
ORIGIN      
        1 ggcgatgcct ggatccctcc gcataccgga ctgcctgcgc actcaacgga cattgccccg
       61 cctgacattc ctgaaggaca cgtggctatc tttgatgagg atgaaaacgc ctggcacctc
      121 atggaagatc accggggtaa agccgtctgg cgtctggagt cggggagcgc cttactgatt
      181 actgagccgg gcccgttacc ggaaaacgtg acagaaataa aaccggacgg ggagttctgc
      241 cggtgggatg gttcatcatg ggttaaggat gaagcagcag aacaggctta ccgggtgagc
      301 gaggcggaga acaaaaaacg ggaattaata caggaagcaa caaatacaat aaacatcctc
      361 caggatgccg tggatttaaa tatggcgacc gacgatgaaa gcgccctgct tcccctgtgg
      421 aaaaaatacc gggtattact gaaccggaca gacacgggca acgcgccgga tattgtctgg
      481 cctgaatccc cttcgccgga tcatcacgca ggctgaccgg aggcaccatg acgtggacaa
      541 gaaaaacaat cagcgtcagc gctgacatgc ccccgctgag ctgttccgtc atccccgtca
      601 caccgtggac ttacgggctg ggacgatatg aagaatcggg ggtatattta agccctccga
      661 acgccataaa ctggctggcc gggaaaatgg ccggaagccg tgcatccggc gacgtgacca
      721 ttatcatgat tgccgaaaac acccacgatc tttttata
//
LOCUS       DAZKHK010000441          756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKHK010000441 DAZKHK010000000
VERSION     DAZKHK010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.444"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>756
                     /locus_tag="ACHOS5_005556"
     CDS             <1..>756
                     /locus_tag="ACHOS5_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc39 family transposase"
                     /protein_id="HFP9780181.1"
                     /translation="IKKHQTRITQMDSQILSLYAKGMTTREIVATFKEMYDADVSPTL
                     ISKVTDAVKEQVSEWQNRPLDALYPIVYLDCIVVKVRHGGSVINKAVFLALGINTDGQ
                     KELLGMWLAENEGAKFWLGVLTELKNRGLQDILIACVDGLKGFPEAINSVYPQTHIQL
                     CIIHMVRNSLKFVSWKDYKAVTSGLKAVYQAPTEEAALMALDKFAGVWDEKYPQISKS
                     WRTHWENLNTFFGYPPDIRKAIYTTNAIESLNSV"
BASE COUNT          202 a          185 c          206 g          163 t
ORIGIN      
        1 attaagaagc atcagacacg catcacccag atggacagcc agattttgtc cctgtatgcc
       61 aaaggcatga ctacgcggga aatcgttgcc acgttcaaag aaatgtacga cgccgatgtc
      121 tcgcccacgc tgatatccaa agtgaccgat gcggttaaag aacaggtctc tgaatggcaa
      181 aatcgaccgt tggatgcact gtatcccatt gtttatcttg actgtattgt ggttaaggtt
      241 cgtcacggcg ggagtgtcat taacaaagcg gtgttcctcg cgctgggcat caataccgac
      301 ggtcagaaag agctgctggg tatgtggctg gcagaaaatg aaggtgcaaa gttctggctg
      361 ggtgtactga ctgagctgaa aaatcgtggc cttcaggaca tcctgattgc ctgcgtggac
      421 ggcctgaaag gcttcccgga agcgataaat agcgtgtacc cgcagacgca tattcagctc
      481 tgcatcatcc atatggtgcg caacagcctg aaattcgtgt cgtggaagga ttacaaagcc
      541 gtcaccagcg ggctgaaagc ggtatatcag gccccgacag aggaagcggc gttgatggcg
      601 ttggataagt ttgcaggcgt ctgggatgaa aaatacccgc aaatcagcaa gagctggcgc
      661 acacactggg aaaatctcaa caccttcttc ggttacccac cggatatccg caaggccatc
      721 tacacgacga atgccatcga atcgctgaac agcgtc
//
LOCUS       DAZKHK010000442          755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKHK010000442 DAZKHK010000000
VERSION     DAZKHK010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.445"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(171..>755)
                     /locus_tag="ACHOS5_005557"
     CDS             complement(171..>755)
                     /locus_tag="ACHOS5_005557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9780182.1"
                     /translation="ISYELTPQEYNAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNA
                     DRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAV
                     VHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFME
                     TLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
BASE COUNT          215 a          164 c          143 g          233 t
ORIGIN      
        1 tataactcac aggattccgg atacaaaaaa cccgcgcatc ggcgggttcg gctgcgtggc
       61 aatgtaacca cttttgtcat gatatgatga tttttacgat tgtaaactgt ttttctgcat
      121 cagggcacga taacctgacc attccccacc ctgtcaggtg gggatttgca ctaaacgacc
      181 ttctcagatg taatctgcct gtacgctacc agctttttat cagtgtactg gacaacatct
      241 ttgatgacca gtttatgtac tgctaaaatt agcgtctcca tgaaagaata atcagggtta
      301 ttatcttttg acggcaaaga taccattaac tcgtcggcat cttttgcata gaaatttctc
      361 ccgtagttga actttccggt acaagaagac ttatgaattg atgctgttat gaaaataacg
      421 gcatttctgt caaagaattc tgtatgaaca actgccacat ggtcatctgc accatactca
      481 taatttctgt attttgcgga gccaaacata tctatcgtaa tggtattttc agggaataca
      541 acaacatcat tactgataaa tgcagaaata ccttcattgc cttcacccgc agtaacaaaa
      601 ggcaaggatc cgggaatacg atctgcgttt ttcagacgct tacctcttat tgcggggcca
      661 aataactttt tgaggttata agtgccccac ataagattat tgctatttaa tagctcaatc
      721 gcgttatatt cctgcggtgt aagctcatag cttat
//
LOCUS       DAZKHK010000443          748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKHK010000443 DAZKHK010000000
VERSION     DAZKHK010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.446"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>748
                     /locus_tag="ACHOS5_005558"
     CDS             <1..>748
                     /locus_tag="ACHOS5_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP9780183.1"
                     /translation="VSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDS
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWMPSCVS"
BASE COUNT          193 a          154 c          229 g          172 t
ORIGIN      
        1 cagtatcagc aagcatgaag gcgctggaag atgcaaggga acgtgcgtta gtggttggct
       61 tttcgctgat tatcatgccg gatatgaccc gctacgctgg tgatggggca gggattaagg
      121 ccattacagg cggtgacaag gtggcaattg acccgaaaca caaagccccc tattcaacgc
      181 gtattccggc agtagtgctg gcggttaaca ataacgccat gtcattcagt gaccgcagcg
      241 gggggatctc acgtcgtcgg gtgatattca atttttcgga agttgtaccg gagaacgaac
      301 gcgattcgat gctggcggaa aaaatagaag gtgagctggc ggtagtgatt cgccatctgc
      361 ttacacggtt tgctgatcag gacgaagcca gacgcctgtt atatgagcag cagaaatctg
      421 aagaagcact ggcgataaag cgagaggggg attcgctggt ggacttctgc ggctatctca
      481 tggcatcggt aatgtgtgat ggcctgttag tgggtaatgc tgaaattgtg ccattcagcc
      541 cacgcaggta tctctatcat gcctatctgg cttatatgag ggcacatggg tttggtaaac
      601 ctgtaacact gacgcgcttc ggtaaagata tgccgggggc aatggcggaa tatggcaggg
      661 agtatatgaa acggaaaacg aagcacggtt tgcgttcaaa cgtgacactg acggaggaat
      721 cagaagactg gatgccatca tgtgtatc
//
LOCUS       DAZKHK010000444          745 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKHK010000444 DAZKHK010000000
VERSION     DAZKHK010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.447"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..683)
                     /locus_tag="ACHOS5_005559"
     CDS             complement(<1..683)
                     /locus_tag="ACHOS5_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780184.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTP"
BASE COUNT          148 a          211 c          221 g          165 t
ORIGIN      
        1 ggtgtccacg tgatggtcat gttgtcatct tccagcatga ccatcgtcag gcgctgaccg
       61 ttcacctcca cacccggtcg cagcgtctga atcatcggga gggtcatact gtgcccgccc
      121 gttgatgcct ggctgaactc gtgcgggatg gtgacggggc gaccggaaaa catcccctgc
      181 gcggcagcag cggccataat gccgccgtcc ggtaacggat gccagatgta atccggtacg
      241 gagaaaatac gccccagact ggccagcagt tcgtagcccg tgccgctgtg tgtgaaatgc
      301 ggaacgggtc tgtcagtgta agccgcgttg tccggcagga tgatttccag cccgctggtc
      361 tcactcagcc actgtgtgac cgtgcgcatt gtcgggtgct gaaatgaaca ggggaagggg
      421 tgatcaagga tccccgccat ttcacgaatc agaagccgca ccgagccgtt ttcagcgggc
      481 tggctgcgtt caacgtatcc ggtaaaccag cgcaccaggt cgtttccgta tccgatatcg
      541 agacgaacgg ggcgcccggt gtagttctgc tctgttctgg ccgtgataaa tccgcgtccg
      601 cacgcgttca gctcaagcat gacggaacaa ctgaccagct gaatggtgtc gttacccagt
      661 aacagacgtt tagtggggtt catggttact ccagcgcatc attgacgggt ttgaggacct
      721 tttcttcgaa ccaggtcatt ttctc
//
LOCUS       DAZKHK010000445          744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKHK010000445 DAZKHK010000000
VERSION     DAZKHK010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.448"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>744
                     /locus_tag="ACHOS5_005560"
     CDS             <1..>744
                     /locus_tag="ACHOS5_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9780185.1"
                     /translation="LIMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDSLSD
                     TANALAENARNAGELGRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTG
                     GAAATTTAELWKMAGVISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQ
                     TVGDDAGKVEKAFEGIAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEA
                     MSLFADVMNARLEQLDKAATPVEKIWDDVK"
BASE COUNT          169 a          177 c          254 g          144 t
ORIGIN      
        1 cttatcatgc tgcagcaggg ggggcagatc agcgattcat tcggtggacc gctcagcctg
       61 cttaccctgc tgaaggagga acttctcggg atcagggatg cctctgaatc atcagaggat
      121 tcgctgtcag atacggcaaa tgcactggct gaaaatgccc ggaatgccgg tgaactggga
      181 cgatttatgt cggtggcccg tgtggcggca ggtggcgggg ttgccgtact ggccgcgctt
      241 gctgctgccg cctggcaggc agagcaggct gaccgggcct tattgcgttc actgaccctg
      301 accggagggg cggctgccac cacaacggca gaattgtgga aaatggccgg ggtgatcagc
      361 gatgaagccg gtggtggtat cagacaggcg gcagaaaatc tggcccgtct ggcagaaagc
      421 gggaaatata ccgccgggca gctacggatc atgggggaaa cctctcagag atggctgcag
      481 acggtggggg acgatgccgg gaaggtggaa aaagcctttg aagggattgc agcagatccg
      541 gtgaaggcgc tggcctccct gaatcagcag tataacttcc tgagcgtttc ccagttacgc
      601 catattgatg agcttgagcg cacgaaaggt aaacaggctg cggtgacgga ggcgatgtcc
      661 ctgtttgcgg atgtcatgaa tgcacgtctg gagcaacttg ataaagcggc cacgccggtg
      721 gaaaaaatct gggacgatgt taaa
//
LOCUS       DAZKHK010000446          744 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKHK010000446 DAZKHK010000000
VERSION     DAZKHK010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.449"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..180
                     /locus_tag="ACHOS5_005561"
     CDS             <1..180
                     /locus_tag="ACHOS5_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFP9780186.1"
                     /translation="SAGAEISRAGGPLESVIYHEQQIRDEQERQARLERERARSWHEE
                     QEREARVWRGPSIGM"
     gene            complement(554..>744)
                     /locus_tag="ACHOS5_005562"
     CDS             complement(554..>744)
                     /locus_tag="ACHOS5_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP9780187.1"
                     /translation="SAETAQQRVKEFHSEKFFHSLRSTTTFAGRKYTNSDMPSPKKMK
                     LMADTISAVYLDGYEGRQ"
BASE COUNT          170 a          157 c          213 g          204 t
ORIGIN      
        1 tcagcaggtg cagagattag ccgggcaggt ggaccacttg agtcggttat ttatcacgaa
       61 cagcagataa gggatgaaca ggaaaggcag gcacgcctgg aacgggagcg tgcgcggtcc
      121 tggcatgaag agcaggaacg ggaagcacgg gtatggcgtg gcccgtcaat agggatgtga
      181 cggtgagttg ttccgggagg ttgccagatg gtaccggaac gcgttaaaaa cgcatccggg
      241 cgcgattctg gcgtgtcgtt tttgttgctg tgttttgcct ctttgccaga tcgcgctcat
      301 tcctcagcct catcccgcga tgtctgcgta tgtcatcaac tgaaaagatg gaaaaaaaac
      361 agtgtcatga ggtttacgca ggcaaaacca gcgttattca catggctgaa tagctatgca
      421 tacggttaaa atttatcagg tgcgatcgcg gcagcttttc gggtggtttg ttgccatttt
      481 tacctgtcag cagccgtgat cgcgctgaac gcatttcagc ggtgcgtaca attaagggat
      541 tatggtaaat cccttactgt ctgccctcgt atccatcgag ataaaccgca gaaatggtgt
      601 ctgccattag tttcattttt ttcggcgaag gcatatcgct gtttgtgtat tttctgccag
      661 caaaggttgt tgtgctccgg agtgagtgaa aaaatttttc tgagtggaat tcttttacac
      721 gttgctgtgc cgtttctgct gata
//
LOCUS       DAZKHK010000447          741 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKHK010000447 DAZKHK010000000
VERSION     DAZKHK010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.450"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>741
                     /locus_tag="ACHOS5_005563"
     CDS             <1..>741
                     /locus_tag="ACHOS5_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9780188.1"
                     /translation="AQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFLTGHHTLFMSM
                     MVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDVAIGHFSTLSY
                     VLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTCLFAGADAVKE
                     LSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLVPNARPALDCP
                     VVFPYAPNAVLVGFLSSFAAGLIGMFTLY"
BASE COUNT          156 a          166 c          192 g          227 t
ORIGIN      
        1 gcgcagaaaa gcttcgggaa agaaatggcg atgattatgt tctttgcgat ggttatcaac
       61 attatgattg cccgtttcac accgtggaaa tttatcttcc tgaccggtca tcacacgttg
      121 tttatgtcga tgatggtagc ggtaattctg gcaacagcag gcatgaccgg cattacgctt
      181 atagccgttg gctctttggt tgttggcgtg gcaatggtct ttttcccggc tattgcgcat
      241 ccgtacatga agaaagtgac cggttctgat gacgttgcca tcggccactt ctcaacatta
      301 tcttatgtat tggccggttt catcggcagc aagtttggta ataaagagca ctcgactgaa
      361 gacatgaatg tgccgaaaag tctgctgttc ttgcgcgaca cgccggttgc tatctctttt
      421 accatgagca taattttcct ggtgacgtgc ctgtttgcgg gtgcagatgc ggtgaaagag
      481 cttagcggtg gtaaaaactg gttcatgttc tctatcatgc aatccattac cttcgcagct
      541 ggcgtgtaca tcattctgca gggtgtgcgc atggtgattg cggagattgt cccggcattc
      601 aaaggtattt cagacaagct ggttccgaat gccagacctg ctctcgattg cccggtcgtc
      661 ttcccttacg cacctaacgc tgtactggtt ggtttcctga gtagctttgc tgcaggttta
      721 atcggtatgt tcacgttgta t
//
LOCUS       DAZKHK010000448          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKHK010000448 DAZKHK010000000
VERSION     DAZKHK010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.451"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>736
                     /gene="druC"
                     /locus_tag="ACHOS5_005564"
     CDS             <1..>736
                     /gene="druC"
                     /locus_tag="ACHOS5_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruC"
                     /protein_id="HFP9780189.1"
                     /translation="NRFTKFHEIAHYENYEYLSSSEDISNRISLNLQAVGLTKNSAET
                     LARLTLATFVSGQIIQFSGSLADIIADAIAIAIGAPRYHIWRVPVGIISDMDSFDFIE
                     TIAESSRCLLLKGANLSAFEIYGAAIRDIVVQRQIHPTNYDHLALIATWKQGPATFPD
                     GGMLAELGPVIDTDTLKMRGLSAILPQLKPGCLAKDKWTNIDGLQLDSVDDYVDELRA
                     LLDEAGFDGGTLWKRMVHIFYTSLIRV"
BASE COUNT          222 a          125 c          152 g          237 t
ORIGIN      
        1 ccaatagatt tacaaaattc catgaaatag cgcactatga aaattatgaa tatctttcat
       61 cctccgaaga catatctaat agaatttctt taaatttaca ggctgttggg ttaacaaaaa
      121 attcagcaga aacattggct agattgacat tagctacctt cgtttctgga caaatcattc
      181 aattcagtgg ctctttggca gatattatcg cggatgcaat tgccattgct attggtgcac
      241 cacgttatca catatggaga gttccagttg gtattatttc tgacatggat tcttttgatt
      301 ttatagagac tatagctgaa tcatctcgct gtctcctttt gaaaggggcc aatctttcag
      361 catttgagat ttatggagcg gcaattagag atatagttgt tcaacggcaa atacatccaa
      421 caaattatga ccatctggca ttgatagcta cttggaaaca aggccctgct acattccctg
      481 atggaggaat gttggccgag ttgggaccag ttattgatac tgatacatta aaaatgcgtg
      541 gtttatctgc tattttaccc caattgaaac caggttgtct tgccaaggat aaatggacaa
      601 atattgatgg actacagctt gatagtgttg atgattatgt agatgaatta agagcattac
      661 tggacgaagc tggatttgat gggggaactt tgtggaagag aatggttcat attttctata
      721 cttcactcat aagagt
//
LOCUS       DAZKHK010000449          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKHK010000449 DAZKHK010000000
VERSION     DAZKHK010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.452"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>709
                     /locus_tag="ACHOS5_005565"
     CDS             <1..>709
                     /locus_tag="ACHOS5_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780190.1"
                     /translation="AQLTDRQKMDLAKERVADEVRAQLATDPELLAVNAMAAKLGDAQ
                     LFNLVTRGTAKTVKSGIVRNATEQGVINGAQNAYSRYQENMALRETAGMDVSPWEGVT
                     DAGIEGTVFGAAMGAPFGAVAGYRGRRQAAEEAAMRDAETVQQDDAAPQPEPVDPVAQ
                     HRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQAKRQAVMNELK
                     AKPRSELLEEYRRLSQKE"
BASE COUNT          182 a          170 c          233 g          124 t
ORIGIN      
        1 gcacagctta ctgatcgcca gaaaatggat ctggcaaaag agcgtgttgc cgatgaggtg
       61 cgcgcgcagc tggcaaccga tcctgaattg ctggctgtga atgccatggc ggcaaaactg
      121 ggtgacgcac aactgtttaa tctggtgaca cgaggcacag cgaagaccgt taaaagcggc
      181 attgtcagaa atgccacgga acagggcgta attaacgggg cacagaacgc ctattcgcgc
      241 tatcaggaaa acatggcatt gcgtgagacc gccggaatgg atgtatcgcc atgggagggg
      301 gtgactgacg cagggattga aggcactgtt ttcggtgctg cgatgggggc tccattcggt
      361 gcggttgccg gatatcgtgg cagacgtcag gccgcagaag aagcagccat gcgtgatgct
      421 gaaaccgtgc agcaggacga cgcagccccg caacccgaac cggttgatcc ggtggcacag
      481 catcgtgaat ccatgcaggg catgaatcgc gagcagcttc tggaacagta tgctgatgcg
      541 gatatggcaa cagagggtga cgcatccgca gctcatcgcc gggaagctgc cagccagttg
      601 ttgaatgaac tggacgaaca ggcgaagcga caggctgtga tgaatgagct gaaggcgaag
      661 ccgcgttctg aactgcttga ggaataccgc agactcagcc agaaagagg
//
LOCUS       DAZKHK010000450          706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKHK010000450 DAZKHK010000000
VERSION     DAZKHK010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.453"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..530)
                     /locus_tag="ACHOS5_005566"
     CDS             complement(<1..530)
                     /locus_tag="ACHOS5_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP9780191.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLSCVPGARQTLPDGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRG
                     KPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGG"
     gene            complement(530..>706)
                     /locus_tag="ACHOS5_005567"
     CDS             complement(530..>706)
                     /locus_tag="ACHOS5_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780192.1"
                     /translation="AGALERLFMQTGVSWSDPLRAQYFSVQFSEGIRRAYRHTLATSP
                     YSSYRNPVRRQRFY"
BASE COUNT          177 a          208 c          169 g          152 t
ORIGIN      
        1 ccaccggcag catccttact gaaagcacga aacaacatcc agtccaccag tgggttaaca
       61 taagcctctt ccagtggaac tggcgtatca tcgtcctgcg tcagaacata cactgcctcc
      121 ggtatccggc ttaccactgc atcaatactt atctctttgt cagggacagg aaacagccag
      181 aatacgcgcg gggacaggtc gttgctgata aagcattccg gttttcccct cagcatgtgc
      241 cagtcgggat actgtgcatc aagcacctcc cgggatactg gtttaatcgc actgccatcg
      301 ctgaggcata tcacgtcaag aagctgtatt gcgccatcag gtaaagtctg gcgtgcgcca
      361 gggacacaac tgagcgtttc caggcttgcg cctgcatccg gccttgccag aatcaccgcc
      421 cgcacagcat cattgtaata atcacacagc tcagccagag gccagcgcac catcatcggg
      481 tcaaccagtt gcgtgttcac tcgtccgatg atttcagtaa tcgtcgtcat cagtaaaacc
      541 tttgtctgcg gacaggattg cggtatgagg agtacggact tgtcgccagt gtatgacgat
      601 atgcccgacg gatcccctca gaaaactgca cagaaaaata ctgtgcgcgt aacgggtctg
      661 accatgaaac accagtctgc atgaacaacc gctcaagtgc ccccgc
//
LOCUS       DAZKHK010000451          706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKHK010000451 DAZKHK010000000
VERSION     DAZKHK010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.454"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..530)
                     /locus_tag="ACHOS5_005568"
     CDS             complement(<1..530)
                     /locus_tag="ACHOS5_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP9780193.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGAILETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGG"
     gene            complement(530..>706)
                     /locus_tag="ACHOS5_005569"
     CDS             complement(530..>706)
                     /locus_tag="ACHOS5_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780194.1"
                     /translation="AGALERLFMQTGVSWSDPLRAQYFSVQFSEGIRRAYRHTLATSP
                     YSSYRNPVRRQRFY"
BASE COUNT          187 a          205 c          162 g          152 t
ORIGIN      
        1 ccaccggcag catccttact gaaagcgcga aacaacatcc agtccaccag tgggttaaca
       61 taagcctctt ccagtggaac tggcgtatca tcgtcctgcg tcagaacata cactgcctcc
      121 ggtatccggc ttaccactgc atcaatactt atctctttgt caggagcagg aaacagccag
      181 aatacgcgcg gggacaggtc gttgctgata aaacattcag gaataccctt cattgtgggc
      241 cactcaggat actgcgcatc cagcacctcc cgggataatg gtctgactgc actaccgtca
      301 ctgaggcata tcacgtcaag aagttgtatt acaccatcgg gcaaaacctg acgggcgcca
      361 ggaacacaac tgattgtttc caggattgcg ccagcatccg gtctcgccag aatcactgcc
      421 ctcacagcat cattgtaata atcgcacaat tcctgcaggg gccagcgaac catcatcggg
      481 tcaaccagtt gtgtattcac acgtccgatg atttctgtaa tcgtcgtcat cagtaaaacc
      541 tctgcctgcg tacagggttg cggtatgaag agtacgggct tgtcgccagt gtatgacgat
      601 atgcccgacg gatcccctca gaaaactgca cagaaaaata ctgtgcgcgt aacggatctg
      661 accatgaaac accagtctgc atgaacaacc gctcaagtgc ccccgc
//
LOCUS       DAZKHK010000452          706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKHK010000452 DAZKHK010000000
VERSION     DAZKHK010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.455"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>706
                     /locus_tag="ACHOS5_005570"
     CDS             <1..>706
                     /locus_tag="ACHOS5_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9780195.1"
                     /translation="LQYTDAFRRSSDIIQRAEEGIKIMKNNMENYRNYQAKGLINKDQ
                     LTNQVALYYQQQNNLLSLSGQNEQNALQITTLESQIQTQAADFDNRIYQMELQRYELQ
                     KELVNTDVEGEIIIRALTDGKVDSLSVTVGQMVNTGDSLLQVIPENIENYYLILWVPN
                     DAVPYISAGDKVNIRYEAFPAEKFGQFSATVKTISRTPASTQEMLTYKGAPQNTPGDS
                     VPWYKVIAMPEKQIIRY"
BASE COUNT          223 a          145 c          165 g          173 t
ORIGIN      
        1 ctgcaataca cagatgcgtt tcgtcgctca tcagatatta tacagcgtgc agaggaaggg
       61 ataaaaataa tgaaaaacaa tatggagaat tacagaaact atcaggcaaa agggctgatt
      121 aataaagatc agttaactaa ccaggtggca ttatattatc agcaacaaaa caatcttctc
      181 agcctgagcg gacagaacga acagaatgcc ctgcagataa ccactctgga gagtcagatt
      241 cagactcagg ctgcagattt tgataaccgt atctaccaga tggaactgca acggtacgag
      301 ttacagaaag aactggttaa cactgatgtg gagggcgaaa tcattatccg ggcgttgact
      361 gacgggaaag ttgactccct gagtgtcact gtcgggcaaa tggtcaatac cggagacagc
      421 cttctgcagg ttattcctga gaacattgaa aactattatc ttattctctg ggtcccgaat
      481 gatgctgttc cttatatttc ggctggtgac aaagtgaata ttcgttatga ggcctttccg
      541 gcagaaaaat ttgggcagtt ctctgctacg gttaaaacta tatccaggac tcctgcgtca
      601 acacaggaaa tgttgaccta taagggagca ccacagaata cgccgggcga ctctgttccc
      661 tggtataaag tcattgcgat gcctgaaaag cagattatca gatatg
//
LOCUS       DAZKHK010000453          701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKHK010000453 DAZKHK010000000
VERSION     DAZKHK010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.456"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            96..692
                     /gene="fimE"
                     /locus_tag="ACHOS5_005571"
     CDS             96..692
                     /gene="fimE"
                     /locus_tag="ACHOS5_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044721.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP9780196.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEA"
BASE COUNT          180 a          138 c          176 g          207 t
ORIGIN      
        1 tgtggttatc tttttatcta ttgggctaat tttgaccgat tgaggtttcc tataggtatt
       61 cattcaaata tatctcagtc aggagtacta ctattgtgag taaacgtcgt tatcttaccg
      121 gtaaagaagt tcaggccatg atgcaggcgg tgtgttacgg ggcaacggga gccagagatt
      181 attgtcttat tctgttggca tatcggcatg ggatgcgtat tagtgaactg cttgatctgc
      241 attatcagga tcttgacctt aatgaaggtc gaataaatat tcgccgactg aagaacggat
      301 tttctaccgt tcacccgtta cgttttgatg agcgtgaagc cgtggaacgc tggacccagg
      361 aacgtgctaa ctggaaaggc gctgaccgga ccgacgctat atttatttct cgccgtggga
      421 gtcggctttc tcgccagcag gcctatcgca ttattcgcga tgccggtatt gaagctggaa
      481 ccgtaacgca gactcatcct catatgttaa ggcatgcttg tggttatgaa ctggcggagc
      541 gtggtgcaga tacccgttta attcaggatt atctcgggca tcgaaatatt cgccatactg
      601 tgcgttatac cgccagtaat gctgctcgtt ttgccggatt atgggaaaga aataatctca
      661 taaacgaaaa attaaaaaga gaagaagctt gatttaactt a
//
LOCUS       DAZKHK010000454          697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKHK010000454 DAZKHK010000000
VERSION     DAZKHK010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.457"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..657
                     /locus_tag="ACHOS5_005572"
     CDS             <1..657
                     /locus_tag="ACHOS5_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9780197.1"
                     /translation="VWQGIKTLFSAFSDLFAPVQLTTGQMNSVTRAGETCGRVVAAAV
                     SMISPALEVVISVIRTLIDIFAIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDL
                     WNVIKNSFNRTWNWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETAVPA
                     LAGNRISAELPPGGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG
                     "
BASE COUNT          174 a          204 c          199 g          120 t
ORIGIN      
        1 gtatggcagg gcataaaaac actgttcagt gccttctccg atctgtttgc accggtacag
       61 ctgaccacgg gacagatgaa cagcgtcacc cgtgccgggg aaacatgcgg tcgcgtggtg
      121 gccgcagcgg taagcatgat atcccccgcg ctggaggtgg ttatcagcgt gatcagaacc
      181 ctgatcgaca tcttcgccat cgtcatcaga gggtggcagg acgtggtggc tgcatttgac
      241 ccgaccgccc cggtcgaatc cttccggaaa atggcgggga tcatcggtaa cgtcttcggg
      301 gacttatgga acgtcattaa aaactcgttt aaccggacgt ggaactggct tgcggaaaag
      361 ctgaacaaaa tccccggtat cagtatcgac atgaagccca cggagcaggt gaacaccatg
      421 acgcggggcg caacaggcag caccgtacct ccgcagattg cagaaaccgc cgtcccggca
      481 ctggcaggca accgcatcag cgcagagctg ccacccggag gcatacgcaa ccagatacgc
      541 acggacacct caacccgtat cgacaacagc cggacgttcg gcaatctgac aatcaacacg
      601 cagaacccga tgacgccggg gcaactggat gaatggacgg agctttacgc gggatgaaca
      661 cgccatcgct ttacatcgac ctgcttatca ctgacgg
//
LOCUS       DAZKHK010000455          681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKHK010000455 DAZKHK010000000
VERSION     DAZKHK010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.458"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..248)
                     /locus_tag="ACHOS5_005573"
     CDS             complement(<1..248)
                     /locus_tag="ACHOS5_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9780198.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQL"
     gene            complement(229..>681)
                     /locus_tag="ACHOS5_005574"
     CDS             complement(229..>681)
                     /locus_tag="ACHOS5_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9780199.1"
                     /translation="EGELNFVLKLAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQ
                     DTQLDAHFSGLMYAVLSLFFGDPDMHPADFSLLAPVCEEEQTEMPDEEDMLMQKAAGV
                     AGGVRFCGVGRGDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          157 a          221 c          159 g          144 t
ORIGIN      
        1 agctgagtga caatatccgt gaactgtgcg ggcagtgtgc gcatggcagc cttatactgt
       61 ccgactgata tcccggcttt ttgtgcagcc agcgcctgtc ggctcagccc ctgttcaaca
      121 gtactggcgg tttttctggc atccgcctcc agactggaaa aatggcggcg tacccgggcc
      181 atctgttcat caaaccgggc cgcatccaga ctcaaatcaa taacaagatc acccgctggc
      241 tgggacatat ctcacacctc cggaaatccc cgctgaagcc atcattaatg caacatcatc
      301 ttcgctgaca tccaccacat ccgcagaagg tgaaatatcg cccctaccca ccccacagaa
      361 ccggacgcct ccggcaactc ctgccgcttt ctgcatcagc atgtcttcct cgtccggcat
      421 ctccgtctgc tcttcctcac acactggcgc aagcagactg aaatccgccg gatgcatatc
      481 cggatcgcca aaaaacaggc tgagtacggc gtacatcagc ccggaaaaat gagcgtccag
      541 ttgggtatcc tgaaaataat gcgtgcagta aaaacgtcgc cagtcggcat attcggtgga
      601 tgtcatcccg gcaagcatgg cgcgccagtc gggtctcccc atctctcgcg ccagtttcag
      661 gacaaagttc agctcgcctt c
//
LOCUS       DAZKHK010000456          681 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKHK010000456 DAZKHK010000000
VERSION     DAZKHK010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.459"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>681
                     /locus_tag="ACHOS5_005575"
     CDS             <1..>681
                     /locus_tag="ACHOS5_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFP9780200.1"
                     /translation="QAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGF
                     KSASDARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDA
                     FGFRGVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKG
                     KAAAHYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSNDGKITSGGDFMTEAQ
                     RVRRIFKD"
BASE COUNT          169 a          147 c          205 g          160 t
ORIGIN      
        1 cacaggcgaa aggtgatgaa aaaacagcac cgtcaccgga tgacaaaatc cgctttgacg
       61 tttaccggaa tacccgcagt ggcgatattt ttatcgctta cggtaaaaac aaaatgcggg
      121 tgagaggtgg ctttaagtca gccagtgatg cgcgtaagta cattgattca catcgtgatg
      181 agcttgttcg tcatgtgaag gagatgcggg agatttcgcg tgaggagcag cgcaacgcca
      241 ccaaccgcga ccgtaccgga ccagaacgcc gcaaggggaa tgtttcaccg gagcagttca
      301 gtgatgcgtt tggtttccgt ggtgtgcagt ttggtaatta tgtggaaggt ccgcgtcgtc
      361 aggctgattt gaaccgggct tatgactcgc tgcatgacct tgcggaagta ctgaatgtac
      421 cgacaaaagc gctttccctg aacggtcgtc ttggcctggc atttggtgcc cgtggtaagg
      481 gtaaggcggc agcacactat gagccgggtg aggtggcaat caacctgaca aaaggtaacg
      541 gaccgggtgc gctggcgcac gaatggttcc attctctgga taattatttt ggtcgttatg
      601 acgtttccaa tgacgggaaa attacgtcag gtggtgactt tatgacggaa gcacagcgtg
      661 tcaggcgcat atttaaagac g
//
LOCUS       DAZKHK010000457          673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKHK010000457 DAZKHK010000000
VERSION     DAZKHK010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.460"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            126..332
                     /locus_tag="ACHOS5_005576"
     CDS             126..332
                     /locus_tag="ACHOS5_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP9780201.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          189 a          146 c          141 g          197 t
ORIGIN      
        1 ataagcaaaa ataagtggtc ggatgagtag taaaattaca caaacagcgg tactattttc
       61 atcagataaa cgaattcatt tttccggtgc tattcaccgg gtagatacaa aagggggaga
      121 aaagtatgga actctacaaa gaatatcctg catggcttat ctttttacgc cgtacttgtg
      181 cggttgcagc gggcgttctg gcgctgcctt tcatgctttt ctggaaagac cgcgcccgct
      241 tttatagcta cctgcaccgc gtctggtcga aaaccagcga taaaccggtg tggatggatc
      301 aggccgaaaa ggcaaccggt gatttttact gattgcctgc gcgcttaagc cagcctcgta
      361 tcagggggct ggtttaagtg ttcaacatca acgtcttcca tgataattaa catggaagac
      421 gtttccatcg tgttattcat ttcatcactg ttttagcatc aagtttcaat agtatccttt
      481 cataggacta ctctggatgc gtttaccttc attgtcgctc taagcaacaa atctccagtt
      541 aggttgcatc cttccttaca cactttcctg aaacttcagg gtaaggtgag aaagcttttc
      601 taattccagc atctaaataa attccgaatt ttgctattaa tatcgctacc gcaataaaaa
      661 cagagagtaa aga
//
LOCUS       DAZKHK010000458          672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKHK010000458 DAZKHK010000000
VERSION     DAZKHK010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.461"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..281)
                     /locus_tag="ACHOS5_005577"
     CDS             complement(<1..281)
                     /locus_tag="ACHOS5_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9780202.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQL"
     gene            complement(331..633)
                     /locus_tag="ACHOS5_005578"
                     /pseudo
     CDS             complement(331..633)
                     /locus_tag="ACHOS5_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          152 a          212 c          155 g          153 t
ORIGIN      
        1 agctgagtga caatatccgt gaactgtgcg ggcagtgtgc gcatggcagc cttatactgt
       61 ccgactgata tcccggcttt ttgtgcagcc agcgcctgtc ggctcagact ctgctcagca
      121 gcatcagcct gttttctgaa agactgactg actttcccgg acatcaaatc agcaagcgca
      181 ctggtttcac ccagctcttt ttttacccgc gctgcctctt cagaaaaacg ggcagaatcc
      241 agtgtcagca cggctgtcag atcggcaaaa ttacctgcca tcgtggtcac ctcctgaaat
      301 gtcctctgat gccatcaata actgtcacaa cctccttccc tcctccccga aacggactcc
      361 accggcgagg cccgccgcct tctgcatcag tacatcattt tcgtccggca tctccgtctg
      421 ctcttcctca cacgctggag caagcagact gaaatccgcc ggatgcatat ccggatcgca
      481 aaaaaacagg ctgagtacgg cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc
      541 ctgaaaataa tgcgtgcagt aaaaacgtcg ccagtcggca tattcggtgg atgtcatccc
      601 ggcaagcatg gcgcgccagt cggctctccc catctcacgc gccagtttca ggacaaagtt
      661 cagctcgcct tc
//
LOCUS       DAZKHK010000459          668 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKHK010000459 DAZKHK010000000
VERSION     DAZKHK010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.462"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..407
                     /locus_tag="ACHOS5_005579"
                     /pseudo
     CDS             1..407
                     /locus_tag="ACHOS5_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          171 a          201 c          195 g          101 t
ORIGIN      
        1 aacaacgggc agggacccgc cgcaacgacg tgaagcgcgg tttgcgaaac ggcgttgctc
       61 cttacatccc ggcgtctttt tgccgggatg ccaggagcgc aggtcacggc gcgacaaggg
      121 tggcgcgacc gttccggtcg ctgcaaaccc ggggttttgc gtcagggatg gaagcccgcc
      181 agggcggaga cagcttcgct ggctccgttc acgacagccc gccccgccag ggggacgccc
      241 ggaaaaggca cagtcaggag aacaacaaaa cagacagtaa tcagagcaac aggaaaaaga
      301 agaacagtag caggaacaac aacatcatca gtaacagaca aatgacaggc atgaaaagac
      361 agaagcccac agcagaacgt ccgcgtaatg aaaggggcgg cgcgtaagcg cggccccttt
      421 cagcgccacc ggcgcgcggt cttttcccgg cgcgggcaga cgggcattta tcctcccgcc
      481 tgcccggcgt cttcaggccc gcagacgaat aatcatctcg gggcacgggt ataagcaaac
      541 atgccgcgcg acagctcccc gaggatgcag aagacgccag caacgtcttc tgctggcgtg
      601 atttgttcag acctctgtcg aaccgatcca tataccattc agataagcat caaatgcatc
      661 attccctt
//
LOCUS       DAZKHK010000460          666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKHK010000460 DAZKHK010000000
VERSION     DAZKHK010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.463"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..267
                     /locus_tag="ACHOS5_005580"
     CDS             <1..267
                     /locus_tag="ACHOS5_005580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780203.1"
                     /translation="IHGRLVRLGVFIIALLALCIALYKFNSCFRTETVVDVIFGLYFT
                     GVLIGLVIMALPPILGIKHLIGRKKLLMTLFAKYHMMKITQSVF"
     gene            333..635
                     /locus_tag="ACHOS5_005581"
     CDS             333..635
                     /locus_tag="ACHOS5_005581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780204.1"
                     /translation="MRVSSFFGEKTAVLSVLGLCYSAVQASIGFDKLSQTFIGDFSNA
                     DSTNTIIMFGLALLLGMSLGALMLKKVASHQLYLKEIVELAIRIKKDVENKGEISE"
BASE COUNT          219 a           86 c          140 g          221 t
ORIGIN      
        1 atacatggta gattagtcag gctcggggtt tttatcatcg ctttacttgc tttatgcatt
       61 gctttgtata aattcaatag ttgttttaga accgagacag ttgtagatgt tatttttggg
      121 ttgtatttta caggggtgct aataggatta gtaataatgg cattgccacc tatcttagga
      181 ataaaacatt tgattggaag aaagaagctt ttaatgactt tatttgcgaa atatcacatg
      241 atgaagataa cgcaaagcgt cttttagatt attcagaaaa agaattattg tatgcgattc
      301 actggataca acttaagatt aatagaataa cgatgagagt tagtagcttt tttggcgaaa
      361 aaacagcagt actctcggta ttgggtctct gctattcggc agtacaggca tcaattggct
      421 ttgataaatt aagtcaaaca tttattggag acttttctaa tgcggattct actaatacta
      481 ttatcatgtt tggattagca cttttgctgg gtatgtcatt gggggcatta atgttgaaaa
      541 aagttgccag ccaccagtta tatctgaaag aaattgttga gttggcaata agaatcaaga
      601 aagatgtcga aaacaaagga gaaatatcgg aataaggtta gtgtcataca taaatatcaa
      661 acatga
//
LOCUS       DAZKHK010000461          647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKHK010000461 DAZKHK010000000
VERSION     DAZKHK010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.464"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            111..>647
                     /locus_tag="ACHOS5_005582"
     CDS             111..>647
                     /locus_tag="ACHOS5_005582"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9780205.1"
                     /translation="MANSGREYEELVRDIQYSLFKAENIPSLKNINIEKNKKIKDRSG
                     IEREFDIYWEFEIGGHTYRSVIECKDYSSPVSIEKIDAFIGKTNDIPGLKLIYATRTG
                     YQSGAKIKAEQHNIQLLVIRDQQEQDWVDEDGTPLLKSIHFRMTAILPPRIVKFNVHV
                     DKEWFYSQNEYTESTLPNL"
BASE COUNT          238 a          102 c          124 g          183 t
ORIGIN      
        1 aatcggttgt ataacttcac atgtaatgct aatattgccc aataaaaagc attgaatatg
       61 ttgttcagtg ctaagttcat caaaaagata atagctataa ggattcttac atggctaatt
      121 caggaagaga atatgaagaa cttgtcagag atatacaata ttctttgttt aaagccgaaa
      181 atattcctag ccttaaaaac attaatatcg agaaaaataa gaagataaaa gatcgttcgg
      241 gaattgaacg agaatttgat atatattggg agttcgaaat tggaggtcat acctatcgtt
      301 cggttatcga gtgtaaggac tattcctccc cggtatcgat tgagaagata gatgccttca
      361 ttggtaaaac taacgatatt ccgggactta aactaatcta tgcgacgcga acgggatatc
      421 aaagcggtgc aaagataaaa gcagagcagc ataacattca actgcttgtt atcagagatc
      481 agcaagaaca agactgggtt gatgaagatg gcactccact tctaaaatct attcatttta
      541 gaatgacagc catattaccg ccaagaatcg ttaaatttaa tgtgcatgtt gataaagaat
      601 ggttttattc acaaaatgaa tatactgaaa gcacattgcc aaatcta
//
LOCUS       DAZKHK010000462          647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKHK010000462 DAZKHK010000000
VERSION     DAZKHK010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.465"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..551)
                     /locus_tag="ACHOS5_005583"
     CDS             complement(<1..551)
                     /locus_tag="ACHOS5_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9780206.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSG"
BASE COUNT          154 a          188 c          158 g          147 t
ORIGIN      
        1 ccactgccgg agccttcata agcaatatca atggccacgg attcggtcgg gttaaactgc
       61 acgccagctc cccacgccag agacgtgttg ctgtggcgac cgtcatcact tccggtcagc
      121 acgtcgtgcg ttttcccctt gttgtcagtt acgcggagat aatccccgga gaaagtcgac
      181 acacggctgt aagccacacc cgccatcgca tacgcgctga accattcatt cacgcgcaca
      241 gacggccccg ccattacgct gaaccagcgg ttacgaacgg aatcttcatg ccagcgggta
      301 tcgctgtaac gggtaagctg gcgattcttg tctcctgcat agctgaaaga cgtcaccatc
      361 cccagtgtgt ccgtaaactc ataacggtat ttcacgttaa tcccgttcag atcatcgctg
      421 ccgggaacgt tcgtcgagac atgaagatac cccgcgctca gcgtggactg atgttcagac
      481 gcccatgcag gcgcaccgga tacggccaga caaatggctg cggacaaaat ggcggcataa
      541 agtttacgca taattacctc tcgcttttct gcaataaaaa aggcgtcatt tctgacgccc
      601 gttatgggtt ataaaattca gctgatactg atacctgctg tggattt
//
LOCUS       DAZKHK010000463          637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKHK010000463 DAZKHK010000000
VERSION     DAZKHK010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.466"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>637
                     /locus_tag="ACHOS5_005584"
     CDS             <1..>637
                     /locus_tag="ACHOS5_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9780207.1"
                     /translation="ASLAAQNTNVVEVDETDKGYQVYGDNWKSIEIGTTKEVKKKNST
                     DLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTGESIAIGAKAKATKEQSIAIG
                     GDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSSSEASGDGSIVVGSRSTAAGDL
                     SVSLGSISSANGTAANALGATAEANGDMATAVGFNALANARNALAVGSQASA"
BASE COUNT          196 a          121 c          177 g          143 t
ORIGIN      
        1 gcatcactgg cagcacaaaa cactaatgta gttgaagttg atgaaacaga taaaggttac
       61 caagtttatg gtgacaactg gaaaagtatt gaaataggta caacgaagga agtaaagaaa
      121 aagaattcaa ccgaccttaa ttttgaccat aactccccgg tagcgaaagg gcataactcc
      181 attgcgattg gtaccagtgc agaagcagga ggtgaaatta aagacgaaaa aggttttaag
      241 acaggagaat ccatagctat tggtgccaag gcgaaagcaa ctaaagaaca gtcaattgca
      301 ataggtggcg ataccaaagc taccggatgg ggggctattg ttattggtgg agatgattta
      361 acccctttgc atggtgacag attcggtgat aaagaaattc cgaataatta ctcgtcatct
      421 gaagcatcag gagacggaag tattgttgtg ggctcaagga gcacagctgc aggagacctg
      481 tctgtgtccc tgggctcaat aagctctgcc aacgggacgg ctgctaatgc actgggagcc
      541 acggcggaag ctaacgggga tatggcgact gcggttggtt ttaatgcgct ggctaatgcc
      601 cgtaatgcgc tggctgttgg tagtcaggcc tctgccg
//
LOCUS       DAZKHK010000464          634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKHK010000464 DAZKHK010000000
VERSION     DAZKHK010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.467"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            28..>634
                     /locus_tag="ACHOS5_005585"
     CDS             28..>634
                     /locus_tag="ACHOS5_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFP9780208.1"
                     /translation="MSRIITTTVYTLHELSSTAQEKARDWYRQHHADSNWYENVYEDF
                     REVCGIFGIDLRQRVFRLSNGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATPRKIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQALT
                     ADAEAAVSEALRDLAFWLYRQLENEYDWLTSDAAVDEAIHIN"
BASE COUNT          150 a          178 c          182 g          124 t
ORIGIN      
        1 ctgaactgag tcagtaaggg cattctgatg agcagaatta tcaccaccac cgtgtataca
       61 ctgcatgagc tatcctccac cgcacaggaa aaagcccgcg actggtaccg tcagcatcat
      121 gctgacagca actggtatga aaacgtgtat gaggacttca gggaggtctg tggcattttc
      181 ggaattgacc tccgccagcg cgttttccgg ctcagcaacg gcaggtttat ggaagagccg
      241 tgcatctggt tcagcggatt ctgcagccag ggggacggag cctgttttga agggcgctgg
      301 cactggcagc cagcgacacc ccggaaaatc agggaatatg cccctcagga ccgtgagctt
      361 catcgtatcg cggatgccct gcaggcggta cagaagcgta acttctggca gttacaggct
      421 gaaatcagtc accggggacg ttactgccat ccgtacagca tggatatcac cgtcacccgg
      481 aacagtccga ctggtcaggc actgacagcc gatgcggaag ccgctgtcag cgaagccctg
      541 cgcgacctgg ccttctggct gtaccgccag ctggaaaacg agtacgactg gctgacctca
      601 gacgccgctg tagatgaggc cattcacatc aatg
//
LOCUS       DAZKHK010000465          634 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKHK010000465 DAZKHK010000000
VERSION     DAZKHK010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.468"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(27..299)
                     /locus_tag="ACHOS5_005586"
     CDS             complement(27..299)
                     /locus_tag="ACHOS5_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP9780209.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFTGKKINRIH"
     gene            complement(342..578)
                     /gene="ycgZ"
                     /locus_tag="ACHOS5_005587"
     CDS             complement(342..578)
                     /gene="ycgZ"
                     /locus_tag="ACHOS5_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP9780210.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          175 a          136 c          105 g          218 t
ORIGIN      
        1 taacctctca tcttacttat gaaattttaa tgtattctgt ttattttctt accagtgaac
       61 atacttttat tgtcagctat cgctgttctt cggtttggcc ctcctgtatc actgttgcta
      121 aaatcacgaa ttatgctgtc aattaaatct cgtcggaaag tattttcctc agccgatttc
      181 attacgagta attgcttaat aagagtctgc aacgaaaaat ttatcttcat gcgcagtatc
      241 tggagcaccg cctggccagt aatttcatat tttatacttt cattatcaga tgtcttcata
      301 acgacttcat agctaacctg ttaaataatc aggctgttgc attattcaaa aagcaaccca
      361 attagtgcat tataatgttt ctgttcctct tcccctgtcg cttgttccag ccggcaaaga
      421 agtttggcac aaagcgactt tcgactcaga ttacgtccat ctttcaaaat ctcagtcaca
      481 atttccccga gagtttcctg ctgagtaggc aagttagctt ttgcaaagta acgagtgatt
      541 gctcctgcag aatctgaagt cactgaattt tgatgcatgc tacgcctctg ttaaaaatgt
      601 taatcaatgt taagtgaggt aacaatagca aact
//
LOCUS       DAZKHK010000466          626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKHK010000466 DAZKHK010000000
VERSION     DAZKHK010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.469"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>626
                     /locus_tag="ACHOS5_005588"
     CDS             <1..>626
                     /locus_tag="ACHOS5_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFP9780211.1"
                     /translation="GDANAAWVDAVKKAQTIASAEGVVIAIDITAKDAVNRATETRAL
                     LQSAYGRFVWFMLCVAGPGKDEAWAAYVTRISAIQDGVAAPGVMVVPRLWGNEPGVLA
                     GRLCNPSVTVADSPARVATGALVAMGNDEIPQDGKKQPLELATLRSLEGKRFSVPMWY
                     HDFDGLYWSDGRTLDVEGGDYQVIENVRVVDKVSRRVRLRAIARLADRA"
BASE COUNT          114 a          184 c          207 g          121 t
ORIGIN      
        1 ggcgacgcta acgccgcatg ggtggacgcg gtgaaaaaag cgcagaccat tgcttctgcc
       61 gagggcgtgg tgattgccat tgacatcacg gccaaagatg ccgttaaccg cgccaccgaa
      121 acccgcgccc tgcttcagtc cgcttatggc cgttttgtct ggttcatgct gtgcgtggcc
      181 ggaccgggca aggatgaggc atgggcggca tacgtcaccc gcatcagtgc catacaggac
      241 ggcgtggcag cacctggcgt gatggtggtt ccccgcttat ggggaaatga gcccggcgta
      301 ctggcgggcc gtctctgtaa tccttccgtc accgttgcgg acagtccggc acgtgtggca
      361 accggtgccc tggtggcgat gggtaacgat gagatcccgc aggacggcaa gaaacagccc
      421 cttgagctgg ccacgctacg ctcgctggaa ggcaaacgct tcagcgtgcc catgtggtat
      481 cacgatttcg acgggctcta ctggtcagac ggacgcaccc ttgatgtgga ggggggcgat
      541 taccaggtca tcgaaaacgt gcgcgtggtg gataaggtct cccgccgtgt acgtctgcgc
      601 gccattgccc gccttgcgga tcgcgc
//
LOCUS       DAZKHK010000467          626 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKHK010000467 DAZKHK010000000
VERSION     DAZKHK010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.470"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>626
                     /locus_tag="ACHOS5_005589"
     CDS             <1..>626
                     /locus_tag="ACHOS5_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HFP9780212.1"
                     /translation="GDANAAWVDAVKKAQTIASAEGVVIAIDITAKDAVNRATETRAL
                     LQSAYGRFVWFMLCVAGPGRDEAWAAYVTRISAIQDGVAAPGVMVVPRLWGNEPGVLA
                     GRLCNPSVTVADSPARVATGALVAMGNDEIPQDGKKQPLELATLRSLEGKRFSVPMWY
                     HDFDGLYWSDGRTLDVEGGDYQVIENVRVVDKVSRRVRLRAIARLADRA"
BASE COUNT          113 a          186 c          207 g          120 t
ORIGIN      
        1 ggcgacgcta acgccgcatg ggtggatgcg gtgaaaaaag cgcagaccat cgcctctgcc
       61 gaaggcgtgg tgattgccat tgacatcacg gccaaagatg ccgttaaccg cgccaccgaa
      121 acccgcgccc tgcttcagtc cgcttatggc cgttttgtct ggttcatgct gtgcgtggcc
      181 ggaccgggca gggatgaggc atgggcggca tacgtcaccc gcatcagtgc catacaggac
      241 ggcgtggcag cccctggcgt gatggtggtt ccccgcttat ggggaaatga gcccggcgta
      301 ctggcgggcc gtctctgtaa tccttccgtc accgttgcgg acagtccggc acgtgtggca
      361 accggtgccc tggtggcgat gggtaacgat gagatcccgc aggacggcaa gaaacagccc
      421 cttgagctgg ccacgctgcg ctcgctggaa ggcaaacgct tcagcgtgcc catgtggtat
      481 cacgatttcg acgggctcta ctggtcagac ggacgcaccc ttgatgtgga ggggggcgat
      541 taccaggtca tcgaaaacgt gcgcgtggtg gataaggtct cccgccgtgt acgtctgcgc
      601 gccattgccc gccttgcaga tcgcgc
//
LOCUS       DAZKHK010000468          616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKHK010000468 DAZKHK010000000
VERSION     DAZKHK010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.471"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..558
                     /locus_tag="ACHOS5_005590"
     CDS             <1..558
                     /locus_tag="ACHOS5_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780213.1"
                     /translation="VPLPWPSDTPPDGYAIMVGQTFDKAQYPLLAKAYPDGRIPDMRG
                     WTIKGKPASGRAVLSQEQDGIKSHTHGGSVSNTDLGTKTTSSFDYGTKTTSANGQHEH
                     SVPLRRQDSGGINFDWLDGSSSGSHIGNGYVPAAGDHQHTVPIGAHTHTLALGSHGHG
                     LTINPTGNAENTVKNIAFNYIVRLA"
BASE COUNT          171 a          183 c          145 g          117 t
ORIGIN      
        1 gttcccctgc cgtggccgtc agacaccccg cctgacggtt acgccatcat ggtggggcag
       61 acattcgata aagcgcaata tcccctgctg gcaaaagcct atccggacgg tcgcatcccg
      121 gatatgcgcg gctggacaat caaggggaaa cctgccagcg gtcgtgcggt attgtcacag
      181 gaacaggacg gcatcaaatc tcacacccac ggggggagtg tctcaaatac tgaccttggc
      241 acgaaaacga cctcatcatt tgattacgga accaaaacaa cctctgcgaa cggacaacat
      301 gaacacagcg tccccctgag aagacaagat agcggaggca taaactttga ctggctggat
      361 ggttcatcgt ccggttctca tattggtaat ggatacgttc ctgccgcagg tgaccaccaa
      421 cataccgtac ccattggcgc acacacccac acgctcgcac tgggttcaca tggtcacggc
      481 ctgaccatta accccaccgg taatgccgaa aacaccgtca aaaacatcgc atttaactac
      541 atcgtgaggc ttgcataatg acctttaaaa tgagcgaaca cccgcagaca attaaaatct
      601 acaacctgtc agccgg
//
LOCUS       DAZKHK010000469          614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKHK010000469 DAZKHK010000000
VERSION     DAZKHK010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.472"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..216
                     /locus_tag="ACHOS5_005591"
     CDS             <1..216
                     /locus_tag="ACHOS5_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780214.1"
                     /translation="NLNEVTLERMITAIKSHFKYYSVYWNSGNFYSIAASNSQKNISD
                     FIMLLPEAAIPMEYVYFKVPTRITGVI"
     gene            221..>614
                     /locus_tag="ACHOS5_005592"
     CDS             221..>614
                     /locus_tag="ACHOS5_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFP9780215.1"
                     /translation="MDFSTLITFSIITLSQTVSIGPGVALIISESLTYGAKKAITTSI
                     YIRFGEIVVMLVTLFSIALAINLEKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKAN
                     IPFLIPMLNPKAFLFFAAFMPSFIKNNSN"
BASE COUNT          212 a          109 c           96 g          197 t
ORIGIN      
        1 aatttaaatg aagtaactct cgaaagaatg attacagcca ttaagtcaca ttttaaatat
       61 tattctgtct actggaatag tggtaatttc tattcaatag ctgccagcaa ttcgcaaaaa
      121 aacatttctg actttataat gttgcttcct gaggcagcca tacctatgga gtatgtttat
      181 tttaaggttc caaccagaat aacaggagtt atataatccc atggacttct caacattaat
      241 aacattctct attataacct tatcacaaac agtatcgatt ggtccggggg tggcattaat
      301 catatctgaa agtctgacat atggtgcaaa aaaagcaata acgacaagta tatatataag
      361 atttggcgaa attgttgtca tgctagtcac actattttct atagcactgg caatcaattt
      421 agaaaaacat cttgacacaa taaaaacttg tggtggtgga tatctgattt ttcttggtat
      481 tactcgtctc atcaagagaa aaggatctaa aggaaacaaa gcaaatatcc cttttttaat
      541 accgatgctc aatccaaagg cctttttgtt ctttgctgca tttatgccat cattcataaa
      601 aaacaacagt aact
//
LOCUS       DAZKHK010000470          611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKHK010000470 DAZKHK010000000
VERSION     DAZKHK010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.473"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..343
                     /locus_tag="ACHOS5_005593"
     CDS             <1..343
                     /locus_tag="ACHOS5_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780216.1"
                     /translation="FTIDTRILQNSNEKITEIFIAKYKDGITYHFGPDMERFQSCFEA
                     ALFAIGEWQIKVDAMTRYHEEDEVMEFFSSIPSDEAGNLATSILLFSDVLAMKGVDEV
                     FAYFLGKDDVV"
     gene            340..>611
                     /locus_tag="ACHOS5_005594"
     CDS             340..>611
                     /locus_tag="ACHOS5_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFP9780217.1"
                     /translation="MSDESKRSRTEKTLKQKVAFAQLELNRLKSLEKSERKKVETRLK
                     IILGAEVAKAMHCDIEQVDKELVMGILLSVSDLNDIERIKYIKAGR"
BASE COUNT          201 a           86 c          146 g          178 t
ORIGIN      
        1 ttttactatt gatactcgta ttcttcagaa cagtaatgaa aagattactg aaattttcat
       61 tgcgaaatat aaagacggaa taacgtatca ttttggccct gatatggaac gatttcagtc
      121 gtgctttgaa gcagcgcttt ttgctattgg tgaatggcaa ataaaagttg atgcgatgac
      181 acgataccat gaggaagatg aagtgatgga gttctttagc tctataccat cagatgaagc
      241 gggaaatctg gcgacgtcta ttttgctgtt cagcgatgtt ctcgccatga aaggcgtgga
      301 tgaagtattt gcctattttt tgggtaagga tgatgttgta tgagtgatga aagtaaaaga
      361 tcaagaacag aaaaaacatt aaaacaaaag gtggcttttg ctcagcttga acttaaccgt
      421 cttaagtctc tggaaaaatc agaacgaaag aaagttgaaa cccggctaaa gattatactt
      481 ggtgcggaag tagctaaagc tatgcattgc gatatcgaac aggtggataa ggaacttgtc
      541 atggggattt tgctttcagt ttctgacctg aatgatattg aaagaataaa gtatataaaa
      601 gcagggagat g
//
LOCUS       DAZKHK010000471          610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKHK010000471 DAZKHK010000000
VERSION     DAZKHK010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.474"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..>610)
                     /locus_tag="ACHOS5_005595"
     CDS             complement(35..>610)
                     /locus_tag="ACHOS5_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP9780218.1"
                     /translation="NRQELISRGLLVPALFLAEFLILVPLYFIFFRKREGLGRGSFRL
                     DVFFTLFSLIMIIQLFVPYLLAVRKTEEWMISQMSFSGAILWINIFSSVLLVPVYEEI
                     VFRGCLFNSFKFWFNDNIYISAIVTSVIFSALHLQYTDFRTFLMLFLISLVLISAKIK
                     SNGLLMPILLHMAMNAVITGIQYTLQYHITV"
BASE COUNT          237 a          102 c          103 g          168 t
ORIGIN      
        1 cttcaggtgt tatagatatc tcataataac aatttcagac tgttatgtga tattgtaacg
       61 tatattgtat tcctgttatg actgcattca tcgccatgtg aagtaatatg ggcattaaca
      121 ggccattcga ctttattttt gcactgataa gaactaacga tatcagaaag agcatcaaaa
      181 atgtacgaaa gtcagtatat tgtagatgta atgcagaaaa tatgactgat gttacaatag
      241 cagagatgta aatattgtca ttaaaccaga atttaaaaga gttaaaaaga caccctcgaa
      301 atacaatctc ttcatataca ggaacaagaa gtacagaaga aaatatatta atccataaaa
      361 tggccccgga aaaagacatt tgcgagatca tccattcttc cgttttcctc actgccagca
      421 gataagggac aaataactga ataatcatta ttaaagagaa aagagtaaaa aaaacatcca
      481 gtcgaaaaga gcctcttcct aatccttctc tttttcggaa gaaaataaaa tacagtggaa
      541 caagaattag aaattcagca agaaataatg ctggaacaag aagtcccctg gatataagtt
      601 cctgcctgtt
//
LOCUS       DAZKHK010000472          610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKHK010000472 DAZKHK010000000
VERSION     DAZKHK010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.475"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..552
                     /locus_tag="ACHOS5_005596"
     CDS             <1..552
                     /locus_tag="ACHOS5_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780219.1"
                     /translation="VPLPWPSDTPPAGYAIMVGQTFDKAQYPLLAKAYPDGRIPDMRG
                     WTIKGKPASGRAVLSQEQDGIKSHTHGGSVSNTDLGTKTTSSFNYGTKTTNSAGPFNA
                     TISLRTATASPEGGGDWTAYGPGSGANLIARVSEHTHSVSIGEHIHTLALGQHGHGLT
                     INPTGNAENTVKNIAFNYIVRLA"
BASE COUNT          173 a          186 c          149 g          102 t
ORIGIN      
        1 gttcccctgc cgtggccgtc agacacccca cctgccggtt acgccatcat ggtggggcag
       61 acattcgata aagcgcaata tcccctgctg gcaaaagcct atccggacgg tcgcatcccg
      121 gatatgcgtg gctggacaat caagggaaaa cctgccagcg gtcgtgcggt attgtcacag
      181 gagcaggacg gcatcaaatc gcacacccac ggagggagcg tctcaaatac tgaccttggc
      241 acaaaaacga cctcatcctt caattacggc acaaaaacaa caaacagcgc ggggccgttc
      301 aacgcaacaa tatcattaag aaccgccacc gcgtcaccgg aaggcggcgg tgactggacc
      361 gcatacgggc cgggctccgg ggcgaacctg atcgccagag tttcggaaca cacacattcg
      421 gtaagcattg gcgaacacat ccacacgctc gcactgggtc aacatggtca cggcctgacc
      481 attaacccca ccggtaatgc ggaaaacacc gtcaaaaaca tcgcatttaa ctacatcgtg
      541 aggcttgcat aatgaccttt aaaatgagcg aacacccaca gacaattaaa atctacaacc
      601 tgtcagccgg
//
LOCUS       DAZKHK010000473          600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKHK010000473 DAZKHK010000000
VERSION     DAZKHK010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.476"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..151)
                     /gene="mchI"
                     /locus_tag="ACHOS5_005597"
     CDS             complement(<1..151)
                     /gene="mchI"
                     /locus_tag="ACHOS5_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFP9780220.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFI"
BASE COUNT          217 a          110 c          110 g          163 t
ORIGIN      
        1 caataaaacc aagaaatatt ataaagctta gaaaaacgtc aacataagaa ttcccttcgc
       61 caacaatccg aagggatgaa agtgaaacca ataagatagt aataggtaaa ctgaatatag
      121 ccgtcaattg ggacagtttt ttataactca ttaatcccta cctgttgctt tattttcatt
      181 atgaaaagtt accaaatctt agcacctcag tcaggagagt caggtcatcg ataaagaata
      241 ttccagtaga gtggcgctca tgatcaaaga taacagcaaa gttgatgaca gcgctgaccg
      301 actgtcattt acagaaaccc tgtttgtagc acattccatc ttacctgacc tcacccagac
      361 aaagacaaaa gagaatgatt gttatttact tttcaaaaaa agtgtcatat tgtccatata
      421 aagtcaagag gtattttacg ttatgtcact gatgcaattg ttagtcagga tctcatggtt
      481 gtcgctcatg tataacctga gacttgaacg cacactaata gcactgacgg tcatacatcg
      541 ggaaatgtga ggaggcataa gaatgaattg tgataataat cacagaaatg aagaattcat
//
LOCUS       DAZKHK010000474          592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKHK010000474 DAZKHK010000000
VERSION     DAZKHK010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.477"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..>592
                     /gene="ompT"
                     /locus_tag="ACHOS5_005598"
     CDS             1..>592
                     /gene="ompT"
                     /locus_tag="ACHOS5_005598"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9780221.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFK"
BASE COUNT          177 a          133 c          142 g          140 t
ORIGIN      
        1 atgcgggcga aacttctggg aatagtcctg acaaccccta ttgcgatcag ctcttttgct
       61 tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag tcttggaact
      121 ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg aaaagtcagt
      181 caacttgact ggaaattcaa taacgctgca attattaaag gtgcaattaa ttgggatttg
      241 atgccccaga tatctatcgg ggctgctggc tggacaacac tcggcagccg aggtggcaat
      301 atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga tgaaagtaga
      361 caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa aggctggctc
      421 ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag ccgttatagc
      481 tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag agatgatatc
      541 ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa aa
//
LOCUS       DAZKHK010000475          582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKHK010000475 DAZKHK010000000
VERSION     DAZKHK010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.478"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>582
                     /gene="ompT"
                     /locus_tag="ACHOS5_005599"
     CDS             <1..>582
                     /gene="ompT"
                     /locus_tag="ACHOS5_005599"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9780222.1"
                     /translation="RAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGKT
                     KERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVD
                     QDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSF
                     TARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQ"
BASE COUNT          171 a          132 c          142 g          137 t
ORIGIN      
        1 tgcgggcgaa acttctggga atagttctga caacccctat tgcgatcagc tcttttgctt
       61 ctaccgagac tatatcgttt actcctgaca acataaatgc ggacattagt cttggaactc
      121 tgagcggaaa aacaaaagag cgcgtttatc tagctgaaga aggaggccga aaagtcagtc
      181 aactcgactg gaaattcaat aacgctgcaa ttattaaagg tgcaattaat tgggatttga
      241 tgccccagat atctattggg gctgcgggct ggactactct cggcagccga ggtggcaata
      301 tggtcgatca ggactggatg gattccagta accccggaac ctggacagat gaaagtaggc
      361 accctgatac acaactcaat tatgccaacg aatttgatct gaatatcaaa ggctggctcc
      421 tcaacgaacc caattaccgc ctgggactca tggccggata tcaggaaagc cgttatagct
      481 ttacagccag aggtggttcc tatatctaca gttctgagga gggattcaga gatgatatcg
      541 gctccttccc gaatggagaa agagcaatcg gctacaaaca ac
//
LOCUS       DAZKHK010000476          580 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKHK010000476 DAZKHK010000000
VERSION     DAZKHK010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.479"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>580)
                     /locus_tag="ACHOS5_005600"
     CDS             complement(<1..>580)
                     /locus_tag="ACHOS5_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9780223.1"
                     /translation="ALHRIRQFQFFGIPPRQVWRELRRLAGQMARNPTAPQRLDHDDI
                     DAILAAADVGCFATYITRQGGVLIPRNTYLVRTAYETAEEANDYGEFPQRIYGVRAPS
                     LGERYTICTHPDTWKLVRRKPENEDRTDEGFDVECGFAAPWTRGNNCPPDQNLTINDP
                     PERQPDGRREQRLTLPEGDLGEWLRSLTPTERK"
BASE COUNT           98 a          159 c          196 g          127 t
ORIGIN      
        1 gttttcgctc tgtgggggtc agtgacctta accattcacc caaatcacct tccggcaggg
       61 taagtcgctg ctccctgcgt ccgtccggct gcctttctgg cgggtcgttt atggtcagat
      121 tctggtcagg gggacagtta ttgccacgag tccaaggtgc ggcaaagccg cactcaacgt
      181 caaaaccctc gtccgtgcgg tcttcgtttt ccggcttacg tctgaccagt ttccaggtgt
      241 ccgggtgggt gcatatggtg tagcgctccc cgagtgacgg ggcgcggacg ccgtaaatgc
      301 gttgcggaaa ttcgccgtag tcgttggcct cttcggcagt ttcgtatgcg gtgcggacca
      361 ggtacgtgtt gcggggaatg agcacaccac cctggcgggt gatataggtg gcaaaacatc
      421 ccacgtcggc ggcggccagt atggcgtcaa tgtcgtcatg atccagacgc tgcggggcag
      481 tcgggttacg ggccatctgc ccggcaaggc ggcggagttc gcgccatacc tggcggggcg
      541 ggataccgaa gaactggaac tggcggatgc ggtgaagggc
//
LOCUS       DAZKHK010000477          577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKHK010000477 DAZKHK010000000
VERSION     DAZKHK010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.480"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>577
                     /locus_tag="ACHOS5_005601"
     CDS             <1..>577
                     /locus_tag="ACHOS5_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780224.1"
                     /translation="SCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFPSPEYII
                     FSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGMAEGAMA
                     RTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEEIKNNNPELFP
                     HGFDIIIEDTTFQMYSPDREQQIKHVKKTLK"
BASE COUNT          209 a          109 c           88 g          171 t
ORIGIN      
        1 aatcatgtta tgcagcacca agaattaaca aaatggctga ttttttctca tcaacagcat
       61 ctggagtaaa acataaacct ttgaagcatg taaaaattga taacaggctt ttcccatctc
      121 ctgagtatat aattttttct gactatctta cgaaaaacag tggtccattt gttaaccact
      181 acttttgcag tattccatac agcaaagaag aagaatgcag gctgggtgtg actattatga
      241 aatatgcaag ccatcgaaat aagccattga gcttatggtg tcttggaatg gcagaaggag
      301 ccatggccag aacaatttct caactatcaa aaggacaggt tttatcatta acaaccagcc
      361 caacaaaaga aaatgaacct gtattcttta gtcatggttc tcatattcac tcttacttta
      421 tttgctcccc attctttctt gttcatgaag aaataaaaaa taacaaccct gaactatttc
      481 ctcatggttt tgatattata atagaagata caacgtttca gatgtattct cccgacagag
      541 aacaacaaat aaaacatgta aaaaaaacat taaaata
//
LOCUS       DAZKHK010000478          565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKHK010000478 DAZKHK010000000
VERSION     DAZKHK010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.481"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          196 a          104 c           89 g          176 t
ORIGIN      
        1 gccctgacgc tgatattttt attttattca tctcctgtct tttaaataaa aagaaaagta
       61 ctcgatcatt gtttgatctc ttttcctcat gatgctgaat ttagatccta tgtttaaaca
      121 tagtttttcc ctttagacat atagcatttg ctcttaaacg tataggaaaa ctatatcctt
      181 gatagcagca cttcgtgatt gttgcgaaca atcactctgc tgaacagggc ttgcccctgt
      241 taataatgga tttatccaaa aaaaagatcg cgtcgaagac gcatataaag taaaagaaaa
      301 attaattaag ctctcacaac aaaagcagcg gttcgtgatt gtatgcaaca atcacttcgc
      361 tgtaataaca tagtaaggaa gataaagttc aataacgtta gtaggtgcca tttatataag
      421 acaccaatca actaaaaaga agaactgttt tctatatttc atccttttaa acgtcatcca
      481 aagcttcgct atttgaataa tgaagaagaa aaatgctctc aagccagtta tagcttaaaa
      541 caaagtacgc actctcggga gttaa
//
LOCUS       DAZKHK010000479          562 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKHK010000479 DAZKHK010000000
VERSION     DAZKHK010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.482"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>562)
                     /locus_tag="ACHOS5_005602"
     CDS             complement(<1..>562)
                     /locus_tag="ACHOS5_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9780225.1"
                     /translation="ADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRLA
                     DDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVNVSQLNRE
                     ISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSYEIDVTGI
                     PVEYTDSQGNPLVNIGGRFYTQTEDAA"
BASE COUNT          116 a          174 c          127 g          145 t
ORIGIN      
        1 gcagcatctt cggtctgtgt gtaaaaacga ccaccaatat tgacaagcgg gttcccctgg
       61 ctgtcggtgt actccaccgg aatccccgtc acatcaatct catagctgta atttcccttg
      121 tcatccttac tgatggctga aacccgggta ttctgtccgt gacggacttc aactgtctgg
      181 gaggaaacag caacagcctc atcaccggat tcagtcttca gtgcccaggt cgttgctgcc
      241 gttgcctgac tgatttcacg gttcagttgt gaaacgttaa cggcgtcctg gtcattcacc
      301 ccgtctttca cgccggaaat aacccggcca gcagcatcaa taccgttcac agtcactgac
      361 gggccatcct tgatggtcag tccgttttta tccatccggg ttccgccaac agaaacactg
      421 ccgtcatctg ccaggcgaat gttatcagcg agtctgaccg tcaggctccc gttctgaaca
      481 gtcgtgctga tattctgccc gtcaccacca accgtgatat gcttcccgag agacgtgttc
      541 accttgcttc cgtcatccgc ct
//
LOCUS       DAZKHK010000480          561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKHK010000480 DAZKHK010000000
VERSION     DAZKHK010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.483"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          195 a           90 c          105 g          171 t
ORIGIN      
        1 caacttatca aagttgactt tttaaggact aaaaaagttt tgccgacttt tgaagccttg
       61 taatcagtta accagataac atgactacaa atataataaa gtattaaata atcttttcat
      121 atttgtcaaa tctaaatctc aactttttac aagtctgtca aataccattt ccaaaaacta
      181 atctagttct caaaagcaat aacgctaaac ctgattgtac gggtctgaga aggtaggagc
      241 gaaagtgttt gattcattct caaggttatt gtcgatgcca aagcggggag tctgcgtaac
      301 atcaactcga agcgcacatc taaggcttat aaaaacagca gtgtaatttt ctttagcaaa
      361 acgagaaaat gtttttatag ctatgatgtt gtctatggga gtgcagaagg gtgtcgcaat
      421 atggaaacgt attgcaattc taagtcatag catgagaagc gtcttgtcct attacgtgta
      481 taaaatttga aaatcatgag attgttttga aactttaata tgcggattag gagaggcgaa
      541 gctctagaaa aaccatacta c
//
LOCUS       DAZKHK010000481          560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKHK010000481 DAZKHK010000000
VERSION     DAZKHK010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.484"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          193 a           90 c          106 g          171 t
ORIGIN      
        1 ttatcaacgt taattgattg ttgtctttat gagcaacgta ctgtaaatcc gtcacttatt
       61 gtttcattgg taataatcac aagaagaaat agatacctta ggtatagtca gatggaacaa
      121 ggattttcaa aataaaatat atatggtatt aatatttccc gatctgtatt caatgattag
      181 ctcaaagatt gaaaatcaag agaaattgtt atagcagtga tacgaaacaa caaaacggaa
      241 tgattgccag ccgttagaaa acaccggaca ctctcgtgta aaaagctacc ctgtctatgg
      301 atatagcgat atcgactcat caagcgtgga ctgaatgata ttcagctcca tatgggcggc
      361 aacctggagg ataaatacga tttcgccgtt ggaatcaagg ttgttctgca atatctacca
      421 gactgcttca atttatctgt tttggcagtg caataatctt aatttaacta tttgaagatg
      481 atgatattgt cagatgccag aaatttagta atctggagaa ccatggttac gatcactaaa
      541 tattaaagta atagtcgata
//
LOCUS       DAZKHK010000482          557 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKHK010000482 DAZKHK010000000
VERSION     DAZKHK010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.485"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>557
                     /locus_tag="ACHOS5_005603"
     CDS             <1..>557
                     /locus_tag="ACHOS5_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9780226.1"
                     /translation="KATFFDDGWLVKKGETWTVNKGVGYVEGLRAELSTHHPMTTPAF
                     PSRIWLNVWWTGTLTSEWATEHEIITADPDVTEERLRKTYPRDGIQQYFCAIAEIDED
                     GNVTDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATP
                     KAIKIAMDNADGRLAKNRNGEDIPN"
BASE COUNT          163 a          156 c          154 g           84 t
ORIGIN      
        1 aaggcaactt tttttgatga cgggtggctg gttaagaaag gcgaaacctg gacagtcaac
       61 aaaggggtgg gttacgtgga ggggctacgc gcagagctga gcactcacca ccccatgacc
      121 acgcccgcct tcccgtcccg tatctggctg aatgtgtggt ggaccggcac gctcaccagt
      181 gaatgggcga cagagcacga aatcataaca gccgacccgg acgttacgga agagcgttta
      241 cggaaaacgt atccgaggga cggcattcag caatattttt gtgccatcgc tgaaattgat
      301 gaagacggca acgtgactga cctccgcccc agaggaaccc cggaagagcg ccgcagtctg
      361 gacgccctga aaaaacacga gaaatcacgc aaccacccgg acgcaacgct gaaagaaaag
      421 ggcttcacac agttaagcag cgccaccacc agtacatcag agacacaggc agccacgcca
      481 aaggctataa agatcgccat ggacaacgcc gatggtcggc ttgcgaaaaa ccgcaatggt
      541 gaggacatcc ccaacaa
//
LOCUS       DAZKHK010000483          549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKHK010000483 DAZKHK010000000
VERSION     DAZKHK010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.486"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..208)
                     /locus_tag="ACHOS5_005604"
     CDS             complement(<1..208)
                     /locus_tag="ACHOS5_005604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780227.1"
                     /translation="MDIRIASFFAVSLFFYISFSGSYSFNPLLSFWGYKYYEAEDDTG
                     VSFVILSKKPLLKASGNRVNLIKLT"
BASE COUNT          212 a           81 c           92 g          164 t
ORIGIN      
        1 cagtaagctt aatcaaatta acacgattac ctgatgcttt tagcagtggt tttttagata
       61 atatgacaaa gcttacacct gtatcgtctt cagcttcata atatttatat ccccaaaatg
      121 ataataatgg attgaaactg taggagcctg aaaaactgat gtaaaaaaac aaacttacag
      181 caaaaaaaga agcaatccta atatccataa aggcatctgg accactgatt aaaggaaata
      241 aataggttaa gaagtaattg gttatttcct tgtccgctgg agaagcggta gttaatttct
      301 tactggttac ggaattatat ttaattgagt gtttaacaat caagaaacac agtaaaagta
      361 taattgcgca caatgataag taagcgaaca taaatttata gtgctttgat atacatgaaa
      421 gaaaagctaa ggtgaaaaat actggagcaa cttagctagc agtaagaaga tatttggcaa
      481 gcttatttaa catattatcc tccttatctg cgctgatacc tgtatataaa aacagtataa
      541 tatcaattt
//
LOCUS       DAZKHK010000484          548 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKHK010000484 DAZKHK010000000
VERSION     DAZKHK010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.487"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>548
                     /locus_tag="ACHOS5_005605"
     rRNA            <1..>548
                     /locus_tag="ACHOS5_005605"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a          121 c          184 g          117 t
ORIGIN      
        1 acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa cggaggagca
       61 cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat aagccagctt
      121 gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc cggtggttct
      181 gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag gctgataccg
      241 cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat cacatcctgg
      301 ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg cgagctgggt
      361 ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga actgaggggg
      421 gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg ttgtcatgcc
      481 aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat ctaagcacga
      541 aacttgcc
//
LOCUS       DAZKHK010000485          542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKHK010000485 DAZKHK010000000
VERSION     DAZKHK010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.488"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..463
                     /locus_tag="ACHOS5_005606"
     CDS             <1..463
                     /locus_tag="ACHOS5_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780228.1"
                     /translation="RSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGH
                     MMVVAGTCERDVEGDTLDTVGGSLTEKIKGVRHSIALAQKLVGDTVKLGNEGINVLTM
                     LTDLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
BASE COUNT          145 a          154 c          144 g           99 t
ORIGIN      
        1 gcggagcctg accagccggg agaccaccat acaggcaacc gacacaacca ccgtcctggg
       61 cgtatccacg cttcaggctg gcgcagtcac ccatatcact gaagagaact acagcatcag
      121 cacgggcggg cacatgatgg tggttgccgg tacctgtgag cgcgacgtgg aaggcgacac
      181 actggacacc gtgggcggca gccttaccga aaagattaag ggcgtccgtc acagtattgc
      241 gctggcgcag aagctggttg gcgatacggt gaagctggga aacgagggca ttaatgtact
      301 gaccatgctg acggaccttg ccgatgtggt ggaagagctg gccgatatta cagcatcaca
      361 tacccacccc aaaacgggca cgtctccgca ggccgcacag ttcagccagg tcgctcagga
      421 gtgccgccag cttaaaaata aatattcacc aataatcgaa taattattca ctttaacagg
      481 ccgcgcaatg tggccttttt tatccccccg ataataaaaa atattcatca ccggacatca
      541 cc
//
LOCUS       DAZKHK010000486          541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKHK010000486 DAZKHK010000000
VERSION     DAZKHK010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.489"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            27..>541
                     /locus_tag="ACHOS5_005607"
     CDS             27..>541
                     /locus_tag="ACHOS5_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFP9780229.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEA"
BASE COUNT          143 a          125 c          121 g          152 t
ORIGIN      
        1 atatctttat atagtgagga cttattatgt ctcagaactt agacgcaacc gcaattaatc
       61 aaatccatgc ccttatttct gctcagggtg ttaatgaaat tatcagtaag attggtgccg
      121 atgctgtggc attgcctgag aatttccgca ttcatgatct ggaaaaattt aatttaaatc
      181 gcttccgttt ccgtggtgca ctttccactg ccagcatcga tgattttacc cgttattcta
      241 aagatcttgc agatgaaggc acccgctgct ttatcgatgc cgataatatg cgtgccgtca
      301 gtgtgcttaa cctgggtact attgatgagc caggtcacgc agataacact gccactctca
      361 aactgaaaaa gacagcaccg ttctctgctc tgttgtctgt taacggcgag cgtaactccc
      421 agaagtcact ggcagaatgg atcgaagact gggccgacta ccttgtgggc tttgatgcta
      481 atggtgacgc cattcaagca acaaaagcgg ctgcggcagt ccgtaaaatc acgattgaag
      541 c
//
LOCUS       DAZKHK010000487          533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKHK010000487 DAZKHK010000000
VERSION     DAZKHK010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.490"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(222..413)
                     /gene="yfgG"
                     /locus_tag="ACHOS5_005608"
     CDS             complement(222..413)
                     /gene="yfgG"
                     /locus_tag="ACHOS5_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP9780230.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            431..>533
                     /locus_tag="ACHOS5_005609"
     CDS             431..>533
                     /locus_tag="ACHOS5_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780231.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNV"
BASE COUNT          174 a           94 c          123 g          142 t
ORIGIN      
        1 aatttattaa aattcatgcg ggaaataatt tataaaaata catcatcaca acatatgtat
       61 taatgatgta tttatttcgc agtcgaacag gataaacatc agtcaggaca aaaacgtaaa
      121 atttaagatg aaaaaatatc tgccgctttg ataatcccaa agagcaaaag catccgacag
      181 cgaggtgccg ttatgttctg tgagagtgga gaaggtaacc gctaacgttg taccggtgat
      241 tcgacggaga gtgtggattg ctgagcttct tttttgctct gatggtgctg ccaggcaccg
      301 acggacgagt agataaaacg gccaaagaag aagataaagc tgatgagcag tacgatacgg
      361 gtcatgcgac tgttaaatcg gtgtcgtttt cgcatactgg ttgcctgact cacaaaaggt
      421 tccttgaagt atgtcccacg ccgtggacgg tacttacatt aaggcacaac aggacaaaat
      481 ggtcaattct tcgttatgta aaaaagcgtc agtggataca tattttaatg tta
//
LOCUS       DAZKHK010000488          533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKHK010000488 DAZKHK010000000
VERSION     DAZKHK010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.491"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..279
                     /locus_tag="ACHOS5_005610"
     CDS             <1..279
                     /locus_tag="ACHOS5_005610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9780232.1"
                     /translation="TVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKGTPS
                     DIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            292..>533
                     /locus_tag="ACHOS5_005611"
     CDS             292..>533
                     /locus_tag="ACHOS5_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9780233.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQYAFGIQGVVP"
BASE COUNT          132 a          107 c          151 g          143 t
ORIGIN      
        1 acagtggttt gcggtaacgg cttaggcacc agcctgatga tggaaatgag cattaaaagc
       61 attttgaagg atttgtccgt cagcgctgat gttgatcatg tcgacctggg ctccgcgaaa
      121 gggacgccga gtgatatcta tgtcggcacc aaagacattg ccgagcagtt agtcgcgcag
      181 tctgtcgcag gcaaaatcgt cgcactggaa aatatgattg ataagaaagc catgagagaa
      241 cgtttatccg tggcattaac cgaattaggc gcactgtaag ctgggggcga tatggatttc
      301 tttcgttttt tgatgagcga tgtactttcc gaacccgcag tgctggtcgg tttaatcgcc
      361 ctgattggcc tgattgcaca gaaaaaaccg gtaaccgaat gcattaaagg taccgtcaag
      421 accattatgg ggtttgtgat tttaggcgca ggggcgggtt tagttgtgtc gtcactgagt
      481 gacttcgcaa acattttcca gtatgccttt ggtattcagg gtgtagtgcc gaa
//
LOCUS       DAZKHK010000489          528 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKHK010000489 DAZKHK010000000
VERSION     DAZKHK010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.492"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(102..425)
                     /locus_tag="ACHOS5_005612"
     CDS             complement(102..425)
                     /locus_tag="ACHOS5_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780234.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
BASE COUNT          176 a           98 c           78 g          176 t
ORIGIN      
        1 ctttaagaac agttgatcgg atttttagaa ttacaaatta ggataatata tcactaaaag
       61 cagcattgct gcataataag ctatgctgct ttagaaatta atcaacttcc actactagaa
      121 gaggatgttg atgagttaaa tcctgagttt aataaacctc cattgtagtt tggattaaaa
      181 cttccaagtc ctatacccgg attatagtta ataccgtttg atggtattgt tattgtccta
      241 tttgaaccat cagcaataac tcctccaata tatcctccaa ttgcacttcc tgctatatcc
      301 gcaccaggga tttttgttgc tttacctaaa aaaccaccag ccgcagtacc tattgcaacg
      361 gatgcatctt tatctaatcc tgcaccatgt attaaagcaa gttcatcggt tgttatattt
      421 ctcatgttat ttcctctttt tattaaagaa agataaaaca aataaaaaca gtacactcgc
      481 aagtaacgaa agaattattt tctttatgaa aagataaagg gttaatgt
//
LOCUS       DAZKHK010000490          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKHK010000490 DAZKHK010000000
VERSION     DAZKHK010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.493"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>523)
                     /locus_tag="ACHOS5_005613"
     CDS             complement(<1..>523)
                     /locus_tag="ACHOS5_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9780235.1"
                     /translation="AYDNAEKRLQKDQNGADIPDKGRFLNNINAVSKTDFADKRGMRY
                     VRVNAPAGAISGKYYPVVVMRSAGSVSELASRVIITTATRTAGDPMNNCEFNGFVMPG
                     GWTDRGRYAYGMFWQYQNNERAIHSIMMSNKGDDLRSVFYVDGAAFPVFAFIEDGLSI
                     SAPGADLVVNDTT"
BASE COUNT          141 a          135 c          112 g          135 t
ORIGIN      
        1 taggtcgtat cattaacaac gagatcagca ccaggtgcgg atattgacag gccatcttcg
       61 ataaacgcaa aaacagggaa agcagcgcca tcaacataga acacagagcg caaatcatcg
      121 cccttattac tcatcattat tgagtggatg gctcgttcat tgttttgata ttgccagaac
      181 attccataag cataacgccc cctgtcagtc cagccaccag gcataacaaa tccgttaaac
      241 tcgcagttat tcatcggatc gcctgcggtt cgcgttgccg tggtgataat gacccttgat
      301 gccagttcgc ttactgagcc agcagaacgc ataacaacaa cagggtaata ttttccagat
      361 attgcacctg caggagcgtt aacccgcaca taacgcatac cacgcttatc agcaaagtct
      421 gttttactga ccgcgttaat gttgttcagg aagcgtccct tatcgggtat atcagcgccg
      481 ttctggtctt tctgcagacg tttctctgca ttgtcatagg ctg
//
LOCUS       DAZKHK010000491          521 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKHK010000491 DAZKHK010000000
VERSION     DAZKHK010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.494"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            91..>521
                     /locus_tag="ACHOS5_005614"
     CDS             91..>521
                     /locus_tag="ACHOS5_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9780236.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSDGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQR"
BASE COUNT          119 a          118 c          176 g          108 t
ORIGIN      
        1 ggtggtcagg ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctgcg
       61 ggcggttttg tcgtttatgg agcgtgagga atgggtaaag gaagcagtaa ggggcatacc
      121 ccgcgcgaag cgaaggacaa cctgaagtcc acgcagttgc tgagtgtgat tgatgccatc
      181 agtgaagggc cgattgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg
      241 ccggtgctgg acagtgatgg gaataccaac atcgccggtg tcacggtggt gttccgggct
      301 ggtgagcagg agcagactcc gccggaggga tttgaatcct ccggctccga gacggtgttg
      361 ggtacggaag tgaaatacga cacgccgatc acccgcgcca tcacgtcggc aaacatcgac
      421 cgactgcgct ttaccttcgg tgtgcaggca ctggtggaaa ccacctcaaa gggggaccgg
      481 aatccgtcgg aagttcgcct gctggttcag atacagcgta a
//
LOCUS       DAZKHK010000492          519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKHK010000492 DAZKHK010000000
VERSION     DAZKHK010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.495"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..182)
                     /locus_tag="ACHOS5_005615"
     CDS             complement(<1..182)
                     /locus_tag="ACHOS5_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780237.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFIFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAT"
     gene            complement(205..>519)
                     /locus_tag="ACHOS5_005616"
     CDS             complement(205..>519)
                     /locus_tag="ACHOS5_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780238.1"
                     /translation="RRISYAFINAQEAGEVAGFIAIFERLGIGYVELLERQPGVDWDV
                     ILVRVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETLNPFIIHYGAYEGEYICWTAR
                     AE"
BASE COUNT          109 a          148 c          143 g          119 t
ORIGIN      
        1 gtagcctggc ggtgcacaat ctgatcttca ggcggcaggg tttcgtcggg ggataccggt
       61 gtttccgggt tgaggtccgg gacgtttgca aaaataaact catccagcac aacgggggtg
      121 ccttccgccg cctgctgtgc cttgagccgg ataaaggcgc tggtaattac agatttcgcc
      181 ataaagtcat actccttcag tcattcactc tgccctggcc gtccagcaga tatactcccc
      241 ttcgtatgcg ccataatgga tgatgaaggg gttgagggtt tccacttcaa aacggtaacg
      301 gcggcatgtc ctgccatatt tgcggatgat ctccagaaga agatcgctgt tgtcggctat
      361 ctggctgtct gtcacccgca ccaggatcac atcccagtcc acgccgggct ggcgctcgag
      421 taactccacg tagccgatgc ccagccgttc aaaaatggcg ataaagcccg ccacctcgcc
      481 agcctcctgt gcattgatga aggcgtaact gatgcggcg
//
LOCUS       DAZKHK010000493          517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKHK010000493 DAZKHK010000000
VERSION     DAZKHK010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.496"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>517
                     /locus_tag="ACHOS5_005617"
     CDS             <1..>517
                     /locus_tag="ACHOS5_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9780239.1"
                     /translation="VSLETTDLSYRYDSQSAQVFSGLNLSVAPGESVAITGASGAGKT
                     TLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDDRLFSGSIRENICGFA
                     EETDDEWMTECAKASHIHDVIMKMPMGYETLIGELGEGLSGGQKQRIFIARALYRKPG
                     ILFMDEATSSLD"
BASE COUNT          158 a           88 c          132 g          139 t
ORIGIN      
        1 gtttcactgg aaaccactga tttaagctac cggtatgaca gccagtcagc acaggtattc
       61 agtggtctga atttgtctgt ggctccggga gaaagtgtgg ctataactgg tgcctccggt
      121 gccggaaaaa ccacattaat gaaagtatta tgtggactgt ttgaaccaga tagtggaaaa
      181 gtactggtta atggcacgga tatacgtcaa cttggaataa ataattatca ccgtatgata
      241 gcctgtgtta tgcaggacga ccggctattt tcaggatcaa ttcgtgaaaa tatctgtggg
      301 tttgcagaag aaacagacga cgaatggatg acagaatgtg ccaaagcaag tcatattcat
      361 gatgtgataa tgaaaatgcc aatggggtat gaaacgttaa taggtgaact gggggaaggt
      421 ctttccggcg gtcaaaaaca gcgtatattc attgcccgag ctttataccg gaaacctgga
      481 atattattta tggatgaggc tacaagttct cttgata
//
LOCUS       DAZKHK010000494          507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKHK010000494 DAZKHK010000000
VERSION     DAZKHK010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.497"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..>507
                     /locus_tag="ACHOS5_005618"
     CDS             2..>507
                     /locus_tag="ACHOS5_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008807102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HFP9780240.1"
                     /translation="MTDNTVGTRIVRIVTDRPTQNVVTQLFSEQKTFFEERYTEGVEF
                     SGGYITSGDEFFVIPDFDDVIAVLDAINNPTSIPPWEPEEISAFNIIALFSGYPEEDG
                     KPVTALIQSFDKRQVIDNRRTILQKMFQASNTFCQSTEHGIVIDNKLSAILAGTELKF
                     KSFHMLRR"
BASE COUNT          172 a           92 c           94 g          149 t
ORIGIN      
        1 aataacagat aatacagttg gcacacgtat tgtcagaatt gttactgaca gaccgactca
       61 aaatgtagtc acacagttat tcagtgaaca aaaaacattt tttgaagaac gctacacaga
      121 aggtgtggaa ttttcaggtg gatacatcac tagtggagat gagttttttg tcattcctga
      181 ctttgatgat gttatcgctg tattagatgc cattaataat cccacatcta tccctccatg
      241 ggagccggaa gaaatatcag ccttcaatat aatcgctctt ttttcaggct atcctgaaga
      301 ggatggaaaa cctgtaactg cattaattca aagcttcgat aaaagacaag ttattgataa
      361 cagaaggaca attttacaaa aaatgtttca ggcaagcaat actttttgcc agtctactga
      421 gcatggtata gtcattgaca ataagctaag cgctattctc gcaggtacag aattaaaatt
      481 taaaagtttc catatgctcc gcagaat
//
LOCUS       DAZKHK010000495          507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKHK010000495 DAZKHK010000000
VERSION     DAZKHK010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.498"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            48..124
                     /locus_tag="ACHOS5_005619"
     tRNA            48..124
                     /locus_tag="ACHOS5_005619"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
     gene            167..242
                     /locus_tag="ACHOS5_005620"
     tRNA            167..242
                     /locus_tag="ACHOS5_005620"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ala,seq:tgc)
     gene            426..>507
                     /locus_tag="ACHOS5_005621"
     rRNA            426..>507
                     /locus_tag="ACHOS5_005621"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          105 c          131 g          128 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tattatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac
      241 catctctgta gtgattaaat aaaaaatact tcagagtgta cctgcaaagg ttcactgcga
      301 agttttgctc tttaaaaatc tggatcaagc tgaaaattga aacactgaac aacgagagtt
      361 gttcgtgagt ctctcaaatt ttcgtaacac gatgatgaat cgaaagaaac atcttcgggt
      421 tgtgaggtta agcgactaag cgtacacggt ggatgccctg gcagtcagag gcgatgaagg
      481 acgtgctaat ctgcgataag cgtcggt
//
LOCUS       DAZKHK010000496          500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKHK010000496 DAZKHK010000000
VERSION     DAZKHK010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.499"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>500
                     /locus_tag="ACHOS5_005622"
     CDS             <1..>500
                     /locus_tag="ACHOS5_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9780241.1"
                     /translation="RGDVAFFVTVTAPSRFHSVNDDGSLNPKYCGATVRDASDYLVYR
                     FFAAVRRAINKAGLGWYGVRTVEPHHDGTPHWHMLVFTSPENEARITEIMRNAAIRED
                     RAELGDDISPRFKCEKIDPAKGTPASYIATYIGKNLDASAFMGNDPKTGKPYVDKESG
                     KTMAETV"
BASE COUNT          125 a          152 c          136 g           87 t
ORIGIN      
        1 cgcggcgacg tggcgttttt cgtcaccgtc acagcccctt cccgttttca cagcgtgaac
       61 gatgacggca gtctgaaccc gaaatactgc ggggcaacgg tacgtgatgc cagtgattat
      121 ctggtttacc gctttttcgc cgccgtccgc agggccatca acaaggccgg gctgggctgg
      181 tacggcgtgc gcacagtaga gccccaccac gacggtacac cgcactggca catgctggta
      241 ttcaccagcc cggaaaacga agcccgtatc actgaaatca tgcgcaacgc cgccatcagg
      301 gaggaccgcg cagagctggg tgatgatatc tccccgcgtt ttaaatgcga aaaaatcgac
      361 ccggcaaaag gcacaccggc aagctatatc gcgacctata tcggtaagaa cctggacgcc
      421 agcgccttca tggggaatga cccgaaaacc ggcaaacctt acgtcgataa ggaatcaggc
      481 aaaaccatgg ccgaaaccgt
//
LOCUS       DAZKHK010000497          497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKHK010000497 DAZKHK010000000
VERSION     DAZKHK010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.500"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>497)
                     /locus_tag="ACHOS5_005623"
     CDS             complement(<1..>497)
                     /locus_tag="ACHOS5_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP9780242.1"
                     /translation="SIAMGNGAEVTRQAAAGSVAVGSHARAGRANTGTYALNGQAVAG
                     RTGSDTAVVSFGQPGQERQLQSVAPGVLSANSTDAVNGSQLHATNRQVAGNTQAINTL
                     GNKFSQLSYRVEELNRDIRGVGASAAAMSGIPQAYLPGKSLMGLGVGGYGGESAIAIG
                     VSRIS"
BASE COUNT          112 a          167 c          118 g          100 t
ORIGIN      
        1 tcggagattc gggacacacc tattgcaatg gcgctttcac caccatatcc accgacaccc
       61 agccccatca gacttttacc cggcagatag gcctgaggta tcccgctcat ggccgcagcc
      121 gatgcgccaa caccacggat atcccggtta agctcttcca cccggtagct cagctgactg
      181 aatttattcc cgagagtgtt tatcgcctgt gtatttccgg caacctgacg gttagttgca
      241 tgcaactgac tgccattgac ggcatccgtt gaattcgcag acaacacacc cggtgccact
      301 gactgcagct gacgctcctg tcccggctga ccaaaagaaa ccaccgcagt atcacttccg
      361 gtacgtcccg caacagcctg accgttcagg gcataagtcc cggtattcgc gcggccagca
      421 cgggcatggc tgccaactgc aacagagccg gcagcagcct gacgcgtgac ttcagcacca
      481 ttacccatgg caatgct
//
LOCUS       DAZKHK010000498          492 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKHK010000498 DAZKHK010000000
VERSION     DAZKHK010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.501"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>492
                     /locus_tag="ACHOS5_005624"
     CDS             <1..>492
                     /locus_tag="ACHOS5_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP9780243.1"
                     /translation="LKDDKGGSATINGGKDNKNGNTLTVKGDGDIAVTVNDKGMTIDG
                     TTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIKTQVAGDKLEISAETASLPV
                     NGGNGKITVNNGDDNKLVTAKNIAEAINQSGWKIKADKDTGGVIEGNGQGTETLIKPG
                     DMVT"
BASE COUNT          182 a           69 c          132 g          109 t
ORIGIN      
        1 gctaaaggat gataagggag gttctgcgac tattaatggt gggaaggata ataagaatgg
       61 taacacttta actgttaaag gtgatggaga tattgcagtt actgttaatg ataaaggaat
      121 gacgattgac ggaacaacgc tgaaaaatga aataaaaaat gatgccatca aaattaaagg
      181 tggagatggt aagacgctga cgaagagtct tggtgacaat gtcgaaatca ctggcaaagg
      241 cccaataaaa acccaggtgg ctggagataa actggagatt tcagcagaga cagcttcgtt
      301 gcctgtaaat ggtggtaacg ggaagattac agtaaacaat ggcgatgaca ataaacttgt
      361 tactgcgaaa aacattgctg aagccataaa ccagtctggc tggaaaatta aagcggataa
      421 agataccggt ggtgttatcg agggaaatgg tcagggaaca gaaacactga taaaacccgg
      481 tgatatggtt ac
//
LOCUS       DAZKHK010000499          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKHK010000499 DAZKHK010000000
VERSION     DAZKHK010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.502"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(63..164)
                     /locus_tag="ACHOS5_005625"
                     /pseudo
     CDS             complement(63..164)
                     /locus_tag="ACHOS5_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(420..>485)
                     /locus_tag="ACHOS5_005626"
     CDS             complement(420..>485)
                     /locus_tag="ACHOS5_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP9780244.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          146 a           97 c           95 g          147 t
ORIGIN      
        1 attaattgta agttcgcagg tattgtccga tcgttgaatc atttgggcgt tataacctaa
       61 atttttaccg tccaataatc aatatttcta ttgtgattgt taaccactgc gtgcattgac
      121 gggattgaag ggtgaaaaat atgtgcaatt gagaagcctt tcattgatgc cagccttgtc
      181 atcatgacaa acaataatag atgcttattt tcctcgcagg gttctttcgc actatcaaaa
      241 aatgctcatt tacccggcat tacagtcaac gaagcaacat tctatggcct acgctgatgt
      301 aaagcacagc gaaaatcctc cttgttgact tcgtctgacc ttatcgcggc gaccagcaat
      361 tattcgtttt gtgatcctca acacaaaatg ctattcgttg cctatgaggg gagaagaatt
      421 tatggaaaac gattgcattc acaaaatgac gtcctgtcag aagagaaaaa gttgacgtta
      481 atcaa
//
LOCUS       DAZKHK010000500          484 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKHK010000500 DAZKHK010000000
VERSION     DAZKHK010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.503"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..241
                     /locus_tag="ACHOS5_005627"
     CDS             <1..241
                     /locus_tag="ACHOS5_005627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780245.1"
                     /translation="FKMREISDHMLDFVKGGMNLNGLPSSTNVIDLRGKDMGTYIDAN
                     GACWAPGTPSIIMYPGGSGPSYSMSSSTSSANSGS"
BASE COUNT          152 a           91 c           93 g          148 t
ORIGIN      
        1 ctttaaaatg agagaaatat cagatcacat gcttgatttc gtgaaaggag ggatgaatct
       61 taatggatta cctagttcta ctaatgtaat agatctacga ggaaaagata tgggaacata
      121 tattgatgct aatggagcat gctgggctcc gggtactcca tccatcatca tgtatccggg
      181 gggaagtgga ccttcttata gtatgagtag ttccacatcc agtgcaaaca gcggcagtta
      241 aataaatacc ctccggcatg gccggagggt atttatgatg tgcctgtaag actctacttc
      301 tgtcgtctct ttacaaaggc atatgatctg tcattttgta ccagacatct taacgagagc
      361 cttttcatat attacttaca tacgtgaaat ctacagtata aagcaaaaat tttattaaac
      421 ctgttttata tttatcatca atacaacaga tttaattccg gaaagtcact catcatccct
      481 tgaa
//
LOCUS       DAZKHK010000501          472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKHK010000501 DAZKHK010000000
VERSION     DAZKHK010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.504"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..465
                     /locus_tag="ACHOS5_005628"
     CDS             <1..465
                     /locus_tag="ACHOS5_005628"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9780246.1"
                     /translation="AHSSGLIGESPNGVPANLVPYLTQVALGKLPVLHIYGNDYDTKD
                     GTGVRDFIHVTDLANGHIAALKKINGHNGFKVYNLGTGRGYSVLEVVRSFEAITGKSI
                     PIEFSPRREGDIAESWANVSAANYELEWFAKKTLNDMLRDAWKWQTLNPNGL"
BASE COUNT          152 a           65 c          108 g          147 t
ORIGIN      
        1 gcacattctt caggtttgat aggagaatct ccgaatggtg ttcctgccaa tttagttcca
       61 tatttaactc aggttgcttt aggtaaattg cccgtattac atatatatgg taatgactat
      121 gataccaagg atggaacggg agtaagagat tttattcatg ttacggattt agctaatgga
      181 cacatagctg cgctgaaaaa aataaatggt cataatggtt ttaaagttta taatcttggt
      241 actgggcgag gttatagtgt gttagaagtc gttagatctt ttgaggctat tacaggaaaa
      301 agtattccaa tcgaattttc gcctcgcaga gaaggtgata tcgctgaaag ttgggcaaat
      361 gtttccgcag caaactatga gttagagtgg ttcgctaaaa agacattaaa tgatatgtta
      421 cgagatgcat ggaaatggca aacgttaaat ccaaatggat tataaagata tt
//
LOCUS       DAZKHK010000502          465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKHK010000502 DAZKHK010000000
VERSION     DAZKHK010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.505"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..407)
                     /locus_tag="ACHOS5_005629"
     CDS             complement(<1..407)
                     /locus_tag="ACHOS5_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9780247.1"
                     /translation="MLSNMKIFITEQQKAELERLHDSSRDGRVRDRIKAILLASEGWS
                     SAMIAQTLRLHQTTIDHHISEFLNKGKLKPENGGSDSKLSAEQTAFLISQLSDNLFHH
                     TRDVIAFVTRTWNIIFSIPGMNKWLHRNGVTYK"
BASE COUNT          121 a           95 c          107 g          142 t
ORIGIN      
        1 tttttgtagg tgacaccatt acgatgcagc cacttattca tccccggaat gctgaaaatg
       61 atgttccatg tccgggtgac aaacgcaata acatcgcggg tatggtgaaa taaattatcg
      121 gataactggc tgattaaaaa ggccgtttgt tcagcactga gtttgctgtc tgagccgccg
      181 ttttcgggct tcagttttcc tttattcagg aattcgctga tatggtgatc aatcgttgtc
      241 tgatgcaggc gtaaagtctg agcaatcatg gcagaactcc agccttcgga agccagaaga
      301 atggctttaa tacgatctct gactcgcccg tcacgactgg aatcatgaag gcgttcaagt
      361 tcagcttttt gttgctcagt aatgaatatt ttcatgttgg acagcatgat cttctgttgc
      421 tgaaaaatca agcatcttca atgatcacgg gtatatatca atttg
//
LOCUS       DAZKHK010000503          464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZKHK010000503 DAZKHK010000000
VERSION     DAZKHK010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.506"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..426)
                     /locus_tag="ACHOS5_005630"
     CDS             complement(<1..426)
                     /locus_tag="ACHOS5_005630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780248.1"
                     /translation="MAQAAASLSTDKMQIPITTDTLPAPVHFSVATMRNAVSAARAAQ
                     ARQLAAQRPDAAGLRRQISSFISRRAGALAGLEEGIRTLAEQKAQAWVFQYRGYHRAA
                     AAAMVKDIPAPTAVHTVAALLAADSLTELGKMIHEPDRTA"
BASE COUNT           71 a          135 c          154 g          104 t
ORIGIN      
        1 agcagtgcgg tctggctcat ggatcatttt ccccagttca gttaatgagt ctgccgccag
       61 gagtgcggca acggtatgta cggcggtcgg tgccgggata tccttcacca tggccgccgc
      121 tgcggcgcgg tgataccccc ggtactgaaa tacccaggcc tgcgccttct gttctgccag
      181 tgttctgatc ccctcttcca gtccggcaag tgcgccagcc cgtctgctga taaagctgct
      241 tatctggcgg cgaagccctg cggcatccgg gcgttgtgct gccagctgcc tggcctgtgc
      301 tgcacgggcg gcgctgactg cgttgcgcat tgtggcaacg gaaaaatgaa caggtgcggg
      361 cagggtgtcg gtggtgatcg ggatttgcat tttgtccgtg ctcagactgg cggcagcctg
      421 tgccatgcgc tgtgcctgcg tgaataccgg cacgggcaga acgg
//
LOCUS       DAZKHK010000504          464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKHK010000504 DAZKHK010000000
VERSION     DAZKHK010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.507"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            39..>464
                     /locus_tag="ACHOS5_005631"
     CDS             39..>464
                     /locus_tag="ACHOS5_005631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780249.1"
                     /translation="MAQAAASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQ
                     ARQLAAQRPDAAGLRRQISRFISRRAGALAGLEDGIRTLAEQKAQAWVFQYRGYHRAA
                     AAAMVKDIPAPTAVHTVAALLAADSLTELGKMIHEPDRTA"
BASE COUNT          102 a          156 c          136 g           70 t
ORIGIN      
        1 ccgttctgcc cgtgccggta ttcacgcagg cacagcgcat ggcacaggct gccgccagtc
       61 tgagcactga caaaatgcaa atcccgatca ccaccgacac cctgcccgca cctgtgcatc
      121 tttccgttgc cacaatgcgc agcgcagtca gtgccgcccg tgcagcacag gccaggcagc
      181 tggcagcaca acgcccggat gccgcagggc ttcgccgcca gataagccgc tttatcagca
      241 gacgggctgg cgcacttgcc ggactggaag acgggatcag aacactggca gaacagaagg
      301 cgcaggcctg ggtatttcag taccgggggt atcaccgcgc cgcagcggcg gccatggtga
      361 aggatatccc ggcaccgacc gccgtacata ctgtggccgc actcctggcg gcagactcat
      421 taaccgaact ggggaaaatg atccatgagc cagaccgcac tgct
//
LOCUS       DAZKHK010000505          459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKHK010000505 DAZKHK010000000
VERSION     DAZKHK010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.508"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..215
                     /locus_tag="ACHOS5_005632"
     CDS             <1..215
                     /locus_tag="ACHOS5_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9780250.1"
                     /translation="EEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQA
                     AALSRTAGVKSIRARIRARISALNAA"
BASE COUNT          107 a          130 c          144 g           78 t
ORIGIN      
        1 atgaggaaat ccgcgcgaag tggttcagat ttgccgggct gtatcttctg cgcgacgagg
       61 cgggcaaacc gagagccacc gcgattaatg atactgccgt actggaaaag gcagacgcat
      121 acctggcaca ggcggcagca ctgagccgta ccgctggcgt aaaaagtatc agggcgcgca
      181 tcagggcacg catcagcgcc cttaatgcag cgtaaacgac tacccgcagg gccgggcggg
      241 cgcgggagga ggcatcagtg acggaaactg tatggccgtg gaatcccgga cagcccgcct
      301 ttttattcat atgaggcacg tcatgagcga accgagcttc agcgtcagcg gtaaaccgct
      361 gaaatccaca ccgcagacgg tgacaaacgg cgagccgttc tggccggacc ttgatctggc
      421 tgagcttcag gggtcacgca cgcttccggc tgatttacc
//
LOCUS       DAZKHK010000506          446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKHK010000506 DAZKHK010000000
VERSION     DAZKHK010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.509"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..439)
                     /locus_tag="ACHOS5_005633"
     CDS             complement(<1..439)
                     /locus_tag="ACHOS5_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780251.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTAFYRQVQEHITERNHTASPAPPVVLRWLREQHGGATIRCLLLLSQASICH
                     LRVSATVDEECSQVVDLLQQAWRGINAGGQCRVERCFRVTRPDT"
BASE COUNT          104 a          126 c          107 g          109 t
ORIGIN      
        1 atgtatcagg ccgggtaacc cgaaaacacc tttccacccg acattgtccg ccagcgttta
       61 tcccccgcca ggcctgctgc agtaaatcca ccacttgcga acattcttca tcaactgtgg
      121 cactgacccg cagatggcaa atgctcgcct ggctgagcag caacaggcat cgtattgttg
      181 ctcctccatg ttgttcccgc aaccagcgta ataccaccgg cggtgctgga ctggcagtgt
      241 gattacgctc agtaatatgt tcttgcacct gtcggtaaaa ggcagtacag aatgcatgaa
      301 tctgttgccc gttgatgttt ccttctgccg aatagagcgt gaagtgaaat gccaacagat
      361 ttggccagtg gtccaccgcc tgctgaacaa aacgccggat gtctcccggc tctgaaatta
      421 aggcttcggt tatttgcacg aaatgt
//
LOCUS       DAZKHK010000507          437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKHK010000507 DAZKHK010000000
VERSION     DAZKHK010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.510"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>437
                     /locus_tag="ACHOS5_005634"
     CDS             <1..>437
                     /locus_tag="ACHOS5_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780252.1"
                     /translation="FYKKTHRHYPYIQKVNGETKAYALCPRCHNPVLLVNRINNQTES
                     KTLYAKHVKHDVMGIASYSQQGYDDCSLANPTNLDAKIKRDINNKSNNEIKDAVKNYF
                     DLLIYSIESHIGINFSDSVLAQMLEDFNACDGHQYRAINLYNL"
BASE COUNT          168 a           69 c           64 g          136 t
ORIGIN      
        1 aattttataa aaaaacgcat agacattatc cctatattca aaaggttaat ggagaaacta
       61 aagcatacgc tttatgccca agatgtcaca atccggtcct tttggttaat agaataaata
      121 atcagacgga atcaaaaact ttatatgcta agcatgttaa acatgatgta atgggtatcg
      181 catcatattc tcaacaaggt tatgatgatt gctcacttgc aaacccaaca aatcttgatg
      241 caaaaatcaa gagagatatt aataataaat caaataatga aataaaagat gctgttaaaa
      301 attatttcga tctacttata tactcaattg aatcacatat aggcatcaat ttttctgatt
      361 ccgttttagc tcaaatgctt gaggatttca acgcttgtga tggacaccag tatagagcga
      421 ttaacttata taattta
//
LOCUS       DAZKHK010000508          434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKHK010000508 DAZKHK010000000
VERSION     DAZKHK010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.511"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>434
                     /locus_tag="ACHOS5_005635"
     CDS             <1..>434
                     /locus_tag="ACHOS5_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4756 family protein"
                     /protein_id="HFP9780253.1"
                     /translation="VNTIKELKNHISIEEYRNEYRKLRSDDVPLIKAQKFKSAHTELR
                     RLEKKRESLIEYFIDELNPISSSKANTSARSTGNLDLFNERVLYRKAISEKSDEEIVA
                     LVIKQRTEAAVEFQRSIEQSLEQLSRISSEFEPSNQKRRKMS"
BASE COUNT          162 a           85 c           78 g          109 t
ORIGIN      
        1 cgtaaataca atcaaagagc taaaaaatca tatttcgata gaagaataca gaaatgaata
       61 tcgcaagctg cgttccgatg atgttccttt gattaaagcc caaaaattta aatctgcaca
      121 taccgaactt cgcagacttg aaaaaaagag agaatctctt attgaatact ttattgatga
      181 gcttaaccca ataagcagtt caaaagctaa cacatcagcg agatcaactg gtaatctcga
      241 tttatttaac gaacgtgtgt tataccgtaa ggccatttca gaaaagagcg atgaagaaat
      301 agtagccttg gtcataaaac aacgtacaga agccgctgtt gagttccaac gttcaattga
      361 acagagtctt gagcaactat ctcgtatctc gtcagagttc gaaccatcaa accaaaagcg
      421 caggaaaatg tctc
//
LOCUS       DAZKHK010000509          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKHK010000509 DAZKHK010000000
VERSION     DAZKHK010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.512"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>429
                     /locus_tag="ACHOS5_005636"
     CDS             <1..>429
                     /locus_tag="ACHOS5_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780254.1"
                     /translation="AQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGVATV
                     QDIFAGGSRGADEVIDTLPDGKNVVTDTVGKGLKATGKAVSDGAKASDEWLTEQMSPG
                     AVRALNTPITEGYDDSAVLLAKGVNLIGALVPDMVAGGVA"
BASE COUNT           96 a           88 c          144 g          101 t
ORIGIN      
        1 ttgcccagtc cgtccgggga acaggggaaa tggcccgtgg actcggtgat gcgatgattc
       61 agagcccggt aaaaacaggg gcgcgtattt taaatgagtt cagccgtatg gggctaccgg
      121 gtgtcgcaac tgttcaggat atttttgccg gtggcagcag gggggctgat gaggtcatcg
      181 ataccctgcc tgatggaaaa aacgtggtca ctgatactgt cggtaaaggt ctgaaagcaa
      241 ccggtaaggc tgtcagtgat ggtgccaaag cctcggatga atggctgaca gaacagatgt
      301 cgccaggtgc tgttcgtgcg ctgaatacgc cgataacaga aggctatgat gattctgcgg
      361 tcttgctggc gaagggtgta aacctgattg gtgcgcttgt acctgatatg gttgctggcg
      421 gtgtggcta
//
LOCUS       DAZKHK010000510          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKHK010000510 DAZKHK010000000
VERSION     DAZKHK010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.513"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(28..234)
                     /locus_tag="ACHOS5_005637"
     CDS             complement(28..234)
                     /locus_tag="ACHOS5_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HFP9780255.1"
                     /translation="MKKKQILDFIQEIKTPCRTSDIASRFEMSAYQARHYLMCLEKEG
                     KIGRTPLRRGARTLWEVAREAEKY"
BASE COUNT          106 a           87 c           69 g          165 t
ORIGIN      
        1 tttatgcaga aaaaatgcat gtttttatta atacttttcc gcctcccttg ctacctccca
       61 taaagtccgg gcaccacgac gaagaggcgt acgccctatt tttccctctt tttcaagaca
      121 cataaggtaa tggcgggcct gataagcact catttcaaaa cgcgaagcaa tatcactcgt
      181 tctgcatggt gtctttattt cctgaataaa atccagtatc tgctttttct tcatcttctg
      241 ttaacactct tagctgtaat cctttttatt atacatcctt acttacgttg aatctcattg
      301 ccatctttta tcattgtaac cacatgaaaa taaaggatgt tagtaatagt agtgctctgt
      361 tttgactgtt tattgcttta ttgagtggtt agtgattggg catatttttg tttctgtgtt
      421 tttaatt
//
LOCUS       DAZKHK010000511          423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZKHK010000511 DAZKHK010000000
VERSION     DAZKHK010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.514"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..>423
                     /locus_tag="ACHOS5_005638"
     CDS             83..>423
                     /locus_tag="ACHOS5_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP9780256.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYINDEDLHRFEGFEGENMDARSWEYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALT"
BASE COUNT           99 a          123 c          121 g           80 t
ORIGIN      
        1 ctgggcatta tgggcagcgc ctttattgaa gaatgctttt gccgtctggc agaccgctac
       61 ctgaagcaga aggaacgcag aaatgaccca taagccttac atttcgcctt ccctgagcct
      121 gtatcacgcg caggcgcatg acgttctgcg gatgctgccg gaaaacagtg tggacgcgct
      181 catcacagac ccgccataca gttcaggtgc cacacacaag gcggggcgga catgccaggg
      241 cagccacgcc aaatacatca acgatgaaga tcttcaccgc tttgaaggct tcgaaggcga
      301 gaacatggac gcccgttcat gggaatactg gacgcatctg tggatgacac aggcatggcg
      361 cacggtacga cccggtggtt acgccctggt cttcaccgac tggcggcagt tgcccgcgct
      421 gac
//
LOCUS       DAZKHK010000512          422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZKHK010000512 DAZKHK010000000
VERSION     DAZKHK010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.515"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>422
                     /locus_tag="ACHOS5_005639"
     CDS             <1..>422
                     /locus_tag="ACHOS5_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP9780257.1"
                     /translation="RVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELA
                     SGKRLQPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEA
                     ITRGLEALYHAGALKTRQDVTEALTAAGFEVVRTTRSS"
BASE COUNT          110 a          104 c          135 g           73 t
ORIGIN      
        1 agggtgatgg agagctttga gcgggtactg atgcccggtc ttgaaaaaaa tcagtacagc
       61 atcctgtggg tggagcacca ggacaagggg cgtcttgagc tgaattttgt catcccgaat
      121 atggagctgg cgagcggaaa acggctccag ccctattacg accgggcgga ccgtccgcga
      181 atcaatgcct ggcagacact ggtgaatcac cattacgggc tgcacgaccc gaacgcaccg
      241 gagaacaggc ggacacttgt caccccgaat aatctgccga aagcgaaaca ggaggccgca
      301 gaagcgatta cgaggggact ggaggctctt taccatgccg gagcgctaaa aacgcgtcag
      361 gatgtcactg aggcgcttac agcggcaggt tttgaggtgg tgaggacaac caggagcagt
      421 at
//
LOCUS       DAZKHK010000513          419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZKHK010000513 DAZKHK010000000
VERSION     DAZKHK010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.516"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>419
                     /locus_tag="ACHOS5_005640"
     CDS             <1..>419
                     /locus_tag="ACHOS5_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780258.1"
                     /translation="FMQNMVISPSMQFQEKDQSGQTSSTANAEQGIKAKELRVTGMIT
                     FDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFSGQIDAQQQTDRLAW
                     QVSFTLRERRSVPEKRQARATPGSSRKQTPQTPGAKGG"
BASE COUNT          105 a          136 c          116 g           62 t
ORIGIN      
        1 ttcatgcaga acatggtgat ttcgccctcg atgcagttcc aggaaaagga ccagtccggt
       61 cagacatcga gcaccgcgaa cgccgagcag ggcatcaagg ccaaagagct tcgcgtaacc
      121 gggatgatca cctttgacaa tgagcgggcc cttcagcgca ttttccagct ggcctctgcc
      181 accaccgggg acggtgccct gaaagtttac cgtatcgcaa acgccaccgc tgccgccatt
      241 aactttcgtg aagggacatt ttccgggcag atagacgcac agcagcagac cgaccgcctc
      301 gcctggcagg tcagcttcac attacgcgaa agacgaagcg taccggaaaa acgccaggca
      361 cgcgccaccc ccggcagcag ccgtaaacag acgccgcaga cgcccggcgc aaaaggcgg
//
LOCUS       DAZKHK010000514          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZKHK010000514 DAZKHK010000000
VERSION     DAZKHK010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.517"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>415)
                     /locus_tag="ACHOS5_005641"
     CDS             complement(<1..>415)
                     /locus_tag="ACHOS5_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9780259.1"
                     /translation="DAFAGYSRQRVLQSASKLRQSGRVAHRCEGNTRRHFPRLTERAQ
                     EPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELESRGLYRRAATVWMAAFRESHS
                     QSERNNFLARRERCLRKSSKRAVSGDEWYLSGNYVW"
BASE COUNT           88 a          127 c          105 g           95 t
ORIGIN      
        1 cccacacgta attccctgac agataccact catcacccga tacagcgcgc ttgctgcttt
       61 tccgtaagca ccgctcacgg cgcgccagaa aattgtttcg ttctgactgg gagtggcttt
      121 cacggaatgc cgccatccac acggttgcag cacgacggta taagcccctg gactccagtt
      181 cttcagcctg gcgggtcagg cacaaaatta cccggggatc gttagtgccg acatagaaat
      241 tgcgcacagg tctggtttca cgaactggtt gtggttccgg ctcctgcgct ctctcagtca
      301 ggcgcgggaa atgtctgcgt gtatttcctt cacaacggtg agccacacgc ccactctgac
      361 gtaacttgct tgctgactgc agaacgcgct gccgtgagta acctgcaaaa gcatc
//
LOCUS       DAZKHK010000515          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZKHK010000515 DAZKHK010000000
VERSION     DAZKHK010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.518"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>415)
                     /locus_tag="ACHOS5_005642"
     CDS             complement(<1..>415)
                     /locus_tag="ACHOS5_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9780260.1"
                     /translation="DAFAGYSRQRVLQSASKLRQSGRVAHRCEGDTRRHFPRLTERAQ
                     EPEPQPVRETRPVRNFYVGTNDPRVILCLTRQAEELESRGLYRRAATVWMAAFRESHS
                     QPERNNFLARREQCLRKSSKRAVSGDEWYLSGNYVG"
BASE COUNT           90 a          128 c          103 g           94 t
ORIGIN      
        1 cccccacgta attccctgac agataccact catcacccga tacagcgcgc ttgctgcttt
       61 tccgtaaaca ctgctcacga cgcgccagaa aattgtttcg ttctggctgg gagtggcttt
      121 cacggaatgc cgccatccac accgttgcag cacgacggta taagcccctg gactctagtt
      181 cttcagcctg gcgggtcagg cacaaaatca cacggggatc gttagtgccg acatagaaat
      241 tgcgcacagg tctggtttca cgaactggtt gtggttccgg ctcctgtgct ctctcagtca
      301 ggcgcgggaa atgtctgcgt gtatcccctt cacaacggtg agccacacgc ccactctgac
      361 gtaacttgct tgctgactgc agaacgcgct gccgtgagta accagcaaaa gcatc
//
LOCUS       DAZKHK010000516          408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZKHK010000516 DAZKHK010000000
VERSION     DAZKHK010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.519"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..381
                     /gene="istB"
                     /locus_tag="ACHOS5_005643"
     CDS             <1..381
                     /gene="istB"
                     /locus_tag="ACHOS5_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFP9780261.1"
                     /translation="RFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLP
                     FSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLDRILHHSHVV
                     QIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          117 a          102 c           99 g           90 t
ORIGIN      
        1 cgcttcacaa cagcagcaga tctgttactt cagttatcta cggcacaacg tcagggccgt
       61 tataaaacga cgcttcagcg tggagtaatg gccccccgcc tgctcatcat tgatgaaata
      121 ggctatctgc cgttcagtca ggaagaagca aaactgttct tccaggtcat tgctaaacgt
      181 tacgaaaaga gcgcaatgat cctgacatcc aatctgccgt tcgggcagtg ggatcaaacg
      241 ttcgccggtg atgcagcact gacctcagcg atgctggacc gtatcttaca ccactcacat
      301 gtcgttcaaa tcaaaggaga aagctatcga ctcagacaga aacgaaaagc cggggttata
      361 gcagaagcta atcctgagta aaacggtgga tcaatattgg gccgttgg
//
LOCUS       DAZKHK010000517          407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZKHK010000517 DAZKHK010000000
VERSION     DAZKHK010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.520"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..169)
                     /locus_tag="ACHOS5_005644"
     CDS             complement(<1..169)
                     /locus_tag="ACHOS5_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9780262.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRW"
BASE COUNT           99 a           97 c          108 g          103 t
ORIGIN      
        1 accagcggct gctgcgcccc agtttgatgg gtttagggaa atggccttca ctgatcaact
       61 tgtaaaacca tttgtcagtc ataccggtga aactggtgat atagttcatg tcgatcaggg
      121 aattatcggc acgcagaaat acgctgtcgt ggcttgttgt agtcaacacg gtcagaatgt
      181 cccctgtgag atttatgaag attgtgcgaa tgcggggaat ctgctgagct gtgctttcag
      241 aactggcctg ttacgggata ccagggatta ccggcgaggt aacgggcttc cggatcatac
      301 acacctcgat gatcgcggac aaaatcactg aacgcccata tcacctcttt aagtatgtct
      361 tcgcagtccg gtaaatgacg atccagcgcc acatcccggg tggtact
//
LOCUS       DAZKHK010000518          407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZKHK010000518 DAZKHK010000000
VERSION     DAZKHK010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.521"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          147 a           85 c           59 g          116 t
ORIGIN      
        1 gctgttattt tctgaaactc tcagaataat ttcaagatta tatattagcc attttgatga
       61 gtaatttcgg ttattggtga acagagcggc tctctctgct cgataattcc gcaagcctga
      121 caaaaccaaa gtgaataaaa atgcaattta accgaataaa agaaaacatc tatcactggt
      181 gctttctgct gtcaatgtac attatctggc ataaaaaatg ctgaaaatga cccgatcact
      241 acatacaccg aattctctac gcttatagcg ttacctcccc cccaaacctt catgagtgaa
      301 aataaaggta aacacatatc caattgattt aattaaaaat aaatcatacc ggcatgtaaa
      361 aatccacaat gtacaaaaaa cgcgccgtta cggaatattt tatctac
//
LOCUS       DAZKHK010000519          396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZKHK010000519 DAZKHK010000000
VERSION     DAZKHK010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.522"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>396
                     /locus_tag="ACHOS5_005645"
     CDS             <1..>396
                     /locus_tag="ACHOS5_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780263.1"
                     /translation="GVYTKVMVRSCLSPTLKARVALTLMYGTAKRFEAWQNSFIFNWY
                     TDSGFSAEDLVSDLIGFYRVFGTGPDPLLLAKPLSYTKALQIWDTYGAPGNFKNTEFT
                     PFLFTTHPPFKKNQLIKKKLPEWLNYIKP"
BASE COUNT          127 a           71 c           71 g          127 t
ORIGIN      
        1 ccggtgttta tacgaaggtt atggttcgtt catgtctttc acccactctt aaagcaagag
       61 ttgccttaac gttaatgtat ggaactgcga agaggtttga agcatggcaa aactcattta
      121 tttttaactg gtatactgat agcggattta gtgcagaaga tttagtctca gacttgattg
      181 gtttttatcg tgtatttgga acaggccccg atccactttt attggcaaaa cctttatctt
      241 acacaaaagc cttacaaata tgggatacat atggagcgcc agggaatttt aaaaatactg
      301 agtttacgcc attcttattt acaacccatc ctccttttaa aaaaaatcaa ctaataaaaa
      361 agaagcttcc agaatggtta aattatataa agccat
//
LOCUS       DAZKHK010000520          390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZKHK010000520 DAZKHK010000000
VERSION     DAZKHK010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.523"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..251
                     /locus_tag="ACHOS5_005646"
     CDS             <1..251
                     /locus_tag="ACHOS5_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780264.1"
                     /translation="IRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETISERLI
                     READTREYELWRPVRRFFSILFLIVLLVCIHSLITTIK"
BASE COUNT          134 a           63 c           71 g          122 t
ORIGIN      
        1 tcatcagaag tttatgtgtt gtgtgtccat ccatattaat ctggtttcag ttttcggtat
       61 atgtcatgag gaaatattac aggcatattc tggtgcgagg aaaggaaacc gaaaccattt
      121 cagaaaggtt gatacgtgag gctgatacac gagaatatga actatggcgt ccggtccgtc
      181 gctttttctc cattcttttt ctcattgttc tattagtttg cattcattca ctgataacaa
      241 ctataaaata aaagttatag ctgttctgat accggagaag aaacgttcat gctatggtct
      301 cctcttcctg ttatataaaa gaatttaaga agacaagtaa gggaatataa ataaaaacaa
      361 caaaaattaa aaatgcaact ataaaaaaca
//
LOCUS       DAZKHK010000521          389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZKHK010000521 DAZKHK010000000
VERSION     DAZKHK010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.524"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..130)
                     /locus_tag="ACHOS5_005647"
     CDS             complement(<1..130)
                     /locus_tag="ACHOS5_005647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780265.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHA"
BASE COUNT           90 a          108 c           80 g          111 t
ORIGIN      
        1 ccgcatgttt ttccgtggta tcttccgtca cgacgtcctg tgcgtgactg tcaatatcca
       61 catccccaag tccttccagc attttttcca gttgttccgg ggtttcttca cccgtaaatt
      121 caaaatccat aaataactcc gcatggtctg tttatcggac agatccgaat ggttgagtaa
      181 ataaggctta tcgctgcccc cgcgaataag cgcaccgctc ccggaacgct tacctccgga
      241 aacaaaaaac cccgtacgat gacggggttc agttgaagcc agagttttca gagcgacatt
      301 tcattcatcc gctgttgtaa cgtatacagc atctgttgct gaagaacgtc ctgctcctgt
      361 tccatattct gtacgccggt aatgatttc
//
LOCUS       DAZKHK010000522          382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZKHK010000522 DAZKHK010000000
VERSION     DAZKHK010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.525"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..>382
                     /locus_tag="ACHOS5_005648"
     CDS             135..>382
                     /locus_tag="ACHOS5_005648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HFP9780266.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEP"
BASE COUNT           93 a          111 c           98 g           80 t
ORIGIN      
        1 accattaccc ttccgtcgcg cgtctttctg ggggtaacaa acgaagaatt ttcacacatc
       61 ctggcacgcc agatgaacgc aatgggctac ggcctggacg ttgacgcaca ggatatcaga
      121 taaggagtca caccgtgagc tggccgaatg taaccgtaag ccatcagaac cgtttcaacg
      181 gtgccacccg tgaagtggag cgcacgctgc tttttgtggg ctatggcaaa aagaacacag
      241 gtaacaccct ttccgtctcc cctgagaccg acctggacga cgtgctggga ccggatgaaa
      301 gcctgcttaa atccacgctt accgccgcaa tcgccaacgg tggccagaac tggtttgcct
      361 atgttcacgt tctgtctgaa cc
//
LOCUS       DAZKHK010000523          382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZKHK010000523 DAZKHK010000000
VERSION     DAZKHK010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.526"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(104..331)
                     /gene="mchB"
                     /locus_tag="ACHOS5_005649"
     CDS             complement(104..331)
                     /gene="mchB"
                     /locus_tag="ACHOS5_005649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFP9780267.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
BASE COUNT          124 a           99 c           63 g           96 t
ORIGIN      
        1 ataaaaacac acttataaat ggatcttcta tctgttttaa acaaaaagga tatattttac
       61 aggtactacc gaaaaagtgc ctgtaaaata aataaaaata taattagcta ccgccaccag
      121 cagaagaact ggcactacca cttcccacgg tcgagccaat tgctgttgtc aaaccggcag
      181 atacggctcc aactacaacc ccaagtggac caccaggtat tccggcgaga gctccaacaa
      241 tagctgcagc acctgcagca ttagctgaag tagctggcgc acctcccgcc ccggaaatat
      301 atcttaactg tgattctgtt atttctcgca tataaactgc atataaactc catcatattt
      361 aacttccgtt tttccagcct ga
//
LOCUS       DAZKHK010000524          372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZKHK010000524 DAZKHK010000000
VERSION     DAZKHK010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.527"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>372
                     /locus_tag="ACHOS5_005650"
     CDS             <1..>372
                     /locus_tag="ACHOS5_005650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9780268.1"
                     /translation="PLAHMALKLGITPPCWPSLEIRPDRRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPR"
BASE COUNT           84 a          108 c          111 g           69 t
ORIGIN      
        1 agccactggc acacatggcg ttaaaactgg gtatcacgcc accctgctgg ccgtcgctgg
       61 aaatcaggcc cgaccgccgc accgcccccg acccggaaca ggtgccgggt gcggtcatgc
      121 gcctttcctg tgcgacatgg tggaacagtc agttaagaca cctccgggac atctggcgtg
      181 aagagctgtt acgcgccgca ggtctggtgt cacgtaagac atcggtatat atcagccatg
      241 aagcgttact ggactggcgc gaaaaacgcc agcgcacccg cgattttctg aaggcctggg
      301 acatcgagaa cgaagaggga gaacggttca gccttgagga tatctactgg gcaggtctct
      361 ctaatccgcg ta
//
LOCUS       DAZKHK010000525          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZKHK010000525 DAZKHK010000000
VERSION     DAZKHK010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.528"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            13..>362
                     /locus_tag="ACHOS5_005651"
     CDS             13..>362
                     /locus_tag="ACHOS5_005651"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
                     /protein_id="HFP9780269.1"
                     /translation="MIFAEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVAS
                     PEGVSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDW
                     KKVGSLPEGLAYGV"
BASE COUNT          102 a           61 c          101 g           98 t
ORIGIN      
        1 gctggttcat cggtgatttt tgcagaacag caaatttata tactaggagg ggagagaaaa
       61 ccggggcttc gttctgttag atcctggacg ggggagcttt cgcatgatcg aattaaatgg
      121 tctgaacttc caccagtagc ttcacccgag ggggtgtcag gagcatatgc ctctgttatt
      181 gatggaaata tctttctggc cggtggtgca tatttcccgg gggcggcaga aaagtatagc
      241 aatggtgaat actggtctca taaaggactg gataaagcct attcaaaaga gatatatcag
      301 ctaataaaaa atgactggaa aaaagttggt tctttgccgg aaggacttgc ttatggtgta
      361 ag
//
LOCUS       DAZKHK010000526          361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZKHK010000526 DAZKHK010000000
VERSION     DAZKHK010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.529"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..99
                     /locus_tag="ACHOS5_005652"
     CDS             <1..99
                     /locus_tag="ACHOS5_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780270.1"
                     /translation="QHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            96..>361
                     /locus_tag="ACHOS5_005653"
     CDS             96..>361
                     /locus_tag="ACHOS5_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFP9780271.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNML"
BASE COUNT           85 a           67 c          123 g           86 t
ORIGIN      
        1 cagcattatc agagttttgt tgagcaactt gggatcaaac agggggcaga cagtgcattg
       61 tctgcccgta aaaaagtgtt taacggaggt ggagtgtgag tgttgttgtt tcggggacgc
      121 tgaaatctcc tgatggtgag gcgatatcag gagcaaatat taccctgacg gcgctgacag
      181 tttcaccgga tgcgctcagc ggcaccagtg cgtcggcagt gacccgtgaa ggtggatatt
      241 acggaatgac gatggatccg ggggagtatg cggtttcggt gacggtgaaa gggaagactg
      301 ctgtctacgg acgtgtgcgt attgagggga ccgaaagtac ggtgacgctc aatatgctgt
      361 t
//
LOCUS       DAZKHK010000527          358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZKHK010000527 DAZKHK010000000
VERSION     DAZKHK010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.530"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..195)
                     /locus_tag="ACHOS5_005654"
     CDS             complement(<1..195)
                     /locus_tag="ACHOS5_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9780272.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSG"
     gene            complement(176..>358)
                     /locus_tag="ACHOS5_005655"
     CDS             complement(176..>358)
                     /locus_tag="ACHOS5_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9780273.1"
                     /translation="TYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFES
                     GLRQSTEEEKSDSRAA"
BASE COUNT           70 a          111 c          100 g           77 t
ORIGIN      
        1 accggataat gccagcgtcg cgagtgcctc ctgggcagtc aggctgtcgc cggacaccgc
       61 atccgtcagg cggctgatcc caagctggcc tgcaagcgca caaaagaaaa cccgcgcata
      121 ggcgggttca agcatcagcg gctcattaaa ggccatgctg gcaatatgcg ggagattacg
      181 cagctctgct gtcactcttc tcctcctctg ttgattgtcg cagcccggat tcaaatgctg
      241 cagccgccca ggcgggcggt ttaagaccgg ctgcacggcg ctccatcgtt tcacggacct
      301 gctgggcaaa aatttcctga tagtcgtcac cgcgttttgc gcactctttc tcgtaggt
//
LOCUS       DAZKHK010000528          357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZKHK010000528 DAZKHK010000000
VERSION     DAZKHK010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.531"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..228
                     /locus_tag="ACHOS5_005656"
     CDS             <1..228
                     /locus_tag="ACHOS5_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 family protein"
                     /protein_id="HFP9780274.1"
                     /translation="IAASQTYFSRTLREMALATEINGVPFPGEVKPPQDGDITIVWTS
                     STHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            230..>357
                     /locus_tag="ACHOS5_005657"
     CDS             230..>357
                     /locus_tag="ACHOS5_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFP9780275.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVP"
BASE COUNT           91 a          107 c           99 g           60 t
ORIGIN      
        1 attgccgcca gccagacata tttcagccgc acgctgcgtg aaatggcgct cgccacggag
       61 attaacggcg taccgttccc cggcgaggtt aaaccgccac aggacgggga tatcaccatc
      121 gtctggacca gcagtacaca cgtgcagatt tacattgtgg tacgccccta cgagagcgca
      181 aaagccattg gcgtaagtat tgaactcgac acagcactgg agatctgaca tggccggaaa
      241 tgaacgtatc agcggcggcg cgtttgatat caactacgac gggatcatga tccacgtgga
      301 aagcgccacc gtcaccatca ccgacaacag cgccgtggca cagacgcgcg gcgtgcc
//
LOCUS       DAZKHK010000529          356 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZKHK010000529 DAZKHK010000000
VERSION     DAZKHK010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.532"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            58..>356
                     /locus_tag="ACHOS5_005658"
     CDS             58..>356
                     /locus_tag="ACHOS5_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9780276.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSF"
BASE COUNT           97 a          102 c           86 g           71 t
ORIGIN      
        1 caaacacccc ctgcatcatc cactctccat ctacgactca ttctgcagag gagtggcgtg
       61 atgatggaac tgcaacatca acgactgatg gcgctcgccg ggcagttgca actggaaagc
      121 cttataagcg cagcgcctgc gctgtcacaa caggcagtag accaggaatg gagttatatg
      181 gacttcctgg agcatctgct tcatgaagaa aaactggcac gtcatcaacg taaacaggcg
      241 atgtataccc gaatggcagc cttcccggcg gtgaaaacgt tcgaagagta tgacttcaca
      301 ttcgccaccg gagcaccgca gaagcaactc cagtcgttac gctcactcag cttcat
//
LOCUS       DAZKHK010000530          354 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZKHK010000530 DAZKHK010000000
VERSION     DAZKHK010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.533"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..225)
                     /locus_tag="ACHOS5_005659"
     CDS             complement(<1..225)
                     /locus_tag="ACHOS5_005659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780277.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
BASE COUNT           79 a          104 c          103 g           68 t
ORIGIN      
        1 ctccccgaaa ctggagattg agttaaaaaa ctgaccgcgc gggtcgaaag catccgccgc
       61 aatccccttg agcagtatct ggcattgcgg cgtcactacc tccccggaga gccggacgac
      121 gaagagagca tcgcccgtgc catctggctg gatgaatatt ttgcccgtac ccgcgccaac
      181 aagaccgctg aaggtatagc gatcgcattt aacggaaggt gacatggcca gccagctgga
      241 tttcattatc aatctgacag accagcttac ccgcccgctg cggcaggttc aggaatccgt
      301 gacgggattc gccgaacgct cgcagggcgc gttcgcgcgt atcggtgtgg gtgt
//
LOCUS       DAZKHK010000531          353 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZKHK010000531 DAZKHK010000000
VERSION     DAZKHK010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.534"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>353)
                     /locus_tag="ACHOS5_005660"
     CDS             complement(<1..>353)
                     /locus_tag="ACHOS5_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780278.1"
                     /translation="TKRNKEKTIVSLHQRRCIEVMMEARMFGHKLEYMSMPSGVEGVA
                     RIQRQTESSVIKLVSNDDIAAHDKSMRLLSPVALSQLSDCLANLTCHVEIDFLGLGKI
                     FLPSSSMLSLDDGEF"
BASE COUNT          118 a           71 c           49 g          115 t
ORIGIN      
        1 ataaattccc cgtcatctaa tgataacata gaagaactag gtaaaaatat tttaccaaga
       61 cctaaaaaat ctatttctac atgacatgtt aagtttgcta agcaatcaga taattgagat
      121 aacgcaacag gagatagtaa ccgcatgctc ttatcatgag ctgcaatgtc atcattagaa
      181 actaacttaa taacactact ttcagtttgt ctttgaattc ttgccactcc ttcaacacca
      241 gaaggcatag acatgtattc taatttatgg ccaaacattc gtgcttccat cataacttca
      301 atgcatcttc tttgatgtaa ggaaacgatg gtcttttctt tattcctctt agt
//
LOCUS       DAZKHK010000532          350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZKHK010000532 DAZKHK010000000
VERSION     DAZKHK010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.535"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>350
                     /locus_tag="ACHOS5_005661"
     CDS             <1..>350
                     /locus_tag="ACHOS5_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP9780279.1"
                     /translation="YRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDED
                     ATQLSDSDLMEQIERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKL
                     IQDTMGVYSAFLGQ"
BASE COUNT           94 a           79 c           98 g           79 t
ORIGIN      
        1 taccgaaagg ataaaaccgg ggagccatac gggttaattt cccgcgccat tccggcacag
       61 gatgaggtga attttcgtcg tatcaagctg acctggctgc ttcaggccaa acgcgtgatt
      121 atggacgagg atgccaccca gttgtcagac agcgacctga tggagcagat cgaacgtccg
      181 gatggcatta ttaaactgaa tccggcccga aaaaatcaga aaagtgtcgc agatgttttt
      241 cgggttgagc aggattttca ggttgccagc cagcagtttc aggtcatgca ggaatcggaa
      301 aaacttatcc aggataccat gggagtgtat tccgcatttc tcgggcagga
//
LOCUS       DAZKHK010000533          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZKHK010000533 DAZKHK010000000
VERSION     DAZKHK010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.536"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>348
                     /locus_tag="ACHOS5_005662"
     CDS             <1..>348
                     /locus_tag="ACHOS5_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780280.1"
                     /translation="RSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGH
                     MMVVAGTCERDVEGDTQDTVGGSLTEKIKGVRHSIAQAQKLVGDTVKLGNEGINVLTM
                     LTDLADVVEELAD"
BASE COUNT           85 a           94 c          114 g           55 t
ORIGIN      
        1 gcggagcctg accagccggg agaccaccat acaggcaacc gacacaacca ccgtcctggg
       61 cgtatccacg cttcaggctg gcgcggtcac ccatatcact gaagagaact acagcatcag
      121 cacgggcggg cacatgatgg tggttgccgg tacctgtgag cgcgacgtgg aaggcgacac
      181 ccaggacacc gtgggcggca gccttaccga aaagattaag ggcgtccgtc acagtattgc
      241 gcaggcgcag aagctggttg gcgatacggt gaagctggga aacgagggca ttaatgtact
      301 gaccatgctg acggaccttg ccgatgtggt ggaagagctg gcagatat
//
LOCUS       DAZKHK010000534          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZKHK010000534 DAZKHK010000000
VERSION     DAZKHK010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.537"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..>343
                     /locus_tag="ACHOS5_005663"
     CDS             3..>343
                     /locus_tag="ACHOS5_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9780281.1"
                     /translation="MTETEQRLFIMTKLSNPWWRLNHLYKIQNEKGELVTFRMRPAQR
                     QLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIVAQDKQAASEIFRTKI
                     AVPFDHLPDWL"
BASE COUNT          102 a           86 c           69 g           86 t
ORIGIN      
        1 aaatgactga gaccgaacaa cgtctgttca ttatgacaaa actgagcaat ccctggtggc
       61 gcctcaatca tctctacaaa atacagaacg aaaaaggtga actggtcacc ttcagaatgc
      121 gaccggcgca acgtcagttg tttcgtaaca tgcataataa aaacattatc ctgaaagcgc
      181 gccagctggg attttccaca gccattgata tttatcttct cgaccaggca ttattcattc
      241 cgcatctcaa atgcgggatc gtcgctcagg ataaacaggc tgccagtgaa attttccgca
      301 caaaaattgc tgtaccgttt gatcatctcc ctgactggct gag
//
LOCUS       DAZKHK010000535          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZKHK010000535 DAZKHK010000000
VERSION     DAZKHK010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.538"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>343)
                     /locus_tag="ACHOS5_005664"
     CDS             complement(<1..>343)
                     /locus_tag="ACHOS5_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780282.1"
                     /translation="ALRDTAGMDVSPWEGVANATIEGAALGAAMGAPFGAVAGYRGRR
                     QAADEAAMREAEVVQQDDAAPQPESVDPVAQHRESMQGMNREQLLEQYADADMATEGD
                     ASATHRREAASQ"
BASE COUNT           57 a          123 c           90 g           73 t
ORIGIN      
        1 ctggctggcg gcttcccggc gatgagttgc ggatgcgtca ccctctgtcg ccatatccgc
       61 atcagcatac tgctccagaa gctgctcgcg attcatcccc tgcatggatt cacgatgctg
      121 cgccaccgga tcaacagatt ctggttgcgg ggctgcgtcg tcctgctgta cgacttcagc
      181 ctcacgcatg gcggcttcat ctgccgcctg acgtctgcca cgatatccgg caaccgcacc
      241 gaatggtgcc cccatcgcag caccaagggc tgcaccttcg atcgttgcgt tagccacgcc
      301 ctcccacggt gacacatcca ttccggcggt atcacgcagt gcc
//
LOCUS       DAZKHK010000536          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZKHK010000536 DAZKHK010000000
VERSION     DAZKHK010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.539"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>343
                     /locus_tag="ACHOS5_005665"
     CDS             <1..>343
                     /locus_tag="ACHOS5_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780283.1"
                     /translation="ALRETAGMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRR
                     QAAEETAMRDAETVQQDDAAPQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGD
                     ASAAHRREAASQ"
BASE COUNT           73 a           91 c          122 g           57 t
ORIGIN      
        1 ggcattgcgt gagaccgccg gaatggatgt gtcaccgtgg gagggcgtgg ctgacgcaac
       61 gatcgaaggt gcagcctttg gtgctgcgat gggggctcca tccggtgcgg ttgccggata
      121 tcgtggcaga cgtcaggccg cagaagaaac cgccatgcgt gatgctgaaa ccgtgcagca
      181 ggacgacgca gccccgcaac cagaatctgt tgatccggtg gcgcagcagc gtgaatccat
      241 gcagggcatg aatcgcgagc agcttctgga gcagtatgct gatgcggata tggcaacaga
      301 gggtgacgca tccgcagctc atcgccggga agctgccagc cag
//
LOCUS       DAZKHK010000537          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZKHK010000537 DAZKHK010000000
VERSION     DAZKHK010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.540"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>341)
                     /locus_tag="ACHOS5_005666"
     CDS             complement(<1..>341)
                     /locus_tag="ACHOS5_005666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780284.1"
                     /translation="YRIILAREFFTPENRKSVPAHDESWVIICDALLGRLMCCMFQGR
                     RYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTMSEMVVMFGYVYRESKPCHLARE
                     MGINTKTVNTFL"
BASE COUNT           82 a          106 c           67 g           86 t
ORIGIN      
        1 agaaaggtgt tcaccgtctt cgtgtttatc cccatttcac gggcaagatg gcagggcttt
       61 gactcccggt acacgtatcc aaacatcacc accatctcgg acatcgtcag aggtcgcgca
      121 tacgtattat gtacccactc accattccgg attttcctgt acgtctccag gcgccccgta
      181 acatcttccg catcaatctg aagataacgg cgtccctgaa acatgcagca catcagcctg
      241 ccaaggaggg catcacagat tatcacccat gactcatcat gtgccggtac actcttcctg
      301 ttctctggtg taaaaaactc cctcgccagt attatcctgt a
//
LOCUS       DAZKHK010000538          341 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZKHK010000538 DAZKHK010000000
VERSION     DAZKHK010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.541"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..216)
                     /locus_tag="ACHOS5_005667"
                     /pseudo
     CDS             complement(41..216)
                     /locus_tag="ACHOS5_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          100 a           72 c           65 g          104 t
ORIGIN      
        1 tttatgaatg aatttacaaa gatagctgcg cctgatgccg ctacactttt gccagaccat
       61 gttctcctct gagggagtaa ctggcgtttt tcatagaatg ccgtactttt ttcaacaact
      121 gccacatcga acggcattta tggtggcgtg aagtgcctgc cataaccgct caatgtggtg
      181 ttcctacgat gaacaaaccg cttgatatat tctcctgatg caaccggaca atatgttcat
      241 gatgggtatc ccgatacata agccttcgtt ttatttttac aaaaagaaat cgttgtcgat
      301 attttatcaa gaaaaaaata attcaggtaa aacgattcaa c
//
LOCUS       DAZKHK010000539          338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZKHK010000539 DAZKHK010000000
VERSION     DAZKHK010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.542"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>338
                     /locus_tag="ACHOS5_005668"
     CDS             <1..>338
                     /locus_tag="ACHOS5_005668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780285.1"
                     /translation="EWDMPNYRGHSLTEIWRGTEDDLADAVLVATTPGQVYGDPVDPG
                     WSGFYWIRFVNAAGVKGPWNAEKGTQAQTQIGVKAIIDQIRDEAAKSPVVSELRKEIK
                     NAQGQAVKDAA"
BASE COUNT           85 a           73 c          110 g           70 t
ORIGIN      
        1 gaatgggata tgcctaatta tcgcggacat tcactgactg aaatctggcg gggtacggag
       61 gatgaccttg ctgatgcagt gctggttgcc acgacgccgg ggcaggttta cggcgatccg
      121 gttgaccctg gctggtcggg attttactgg atacgttttg ttaacgcggc aggagtgaaa
      181 ggtccatgga atgctgaaaa aggcactcag gcacaaacac agatcggcgt gaaggccatc
      241 attgaccaga tccgcgatga ggctgcaaag tcgccggttg tgtccgagct gcgtaaagaa
      301 ataaaaaacg cgcaggggca ggctgtaaag gatgctgc
//
LOCUS       DAZKHK010000540          335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZKHK010000540 DAZKHK010000000
VERSION     DAZKHK010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.543"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>335
                     /locus_tag="ACHOS5_005669"
     CDS             <1..>335
                     /locus_tag="ACHOS5_005669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780286.1"
                     /translation="VVLYLSHEEFPKGLEDLPRSLIRLHKRGVDINFTCENIRSYKKL
                     HYALSDFPELPVITADDDVLYPSRWVNDFMESHKLFHDDILFARGHQITFDRNGNVKK
                     YISFGKPAG"
BASE COUNT          107 a           47 c           68 g          113 t
ORIGIN      
        1 agtagtattg tacttgtctc atgaagagtt tccaaagggt ttagaggatt tacctcgtag
       61 tttaatacga ttacataaaa gaggtgtcga tattaatttt acgtgtgaaa atattcgatc
      121 ttataagaag ttacattatg cgctgagtga tttcccagaa ctacctgtaa taacagctga
      181 tgatgatgtc ttatatccct cgcgatgggt aaatgatttt atggagtcac ataagctttt
      241 tcatgatgat atattatttg cgaggggtca tcagataaca tttgaccgaa atggaaatgt
      301 taaaaaatat atctctttcg gaaaaccagc tggat
//
LOCUS       DAZKHK010000541          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZKHK010000541 DAZKHK010000000
VERSION     DAZKHK010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.544"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..>327
                     /locus_tag="ACHOS5_005670"
     CDS             108..>327
                     /locus_tag="ACHOS5_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFP9780287.1"
                     /translation="MTTKSTGTITLTVAGVTLTFEPNKTAFNNLINEITMTNKIAPMT
                     TYLNRIVTADCRDALADLMDRYPGCEMQI"
BASE COUNT           91 a          105 c           79 g           52 t
ORIGIN      
        1 ggcgctgatt ttgatgcggc actgcgaacc ctcacgagcc gctacaccga atgcgccgcc
       61 aggcataaca gcctggtgga aaccatacga aacacggaga aactacaatg accacgaaga
      121 gcaccggcac catcaccctg accgtcgcgg gcgtaaccct gacctttgag ccgaacaaga
      181 cggcatttaa caacctcatc aacgaaatca cgatgaccaa caagatagcg ccaatgacca
      241 cctatcttaa ccgtatcgtg accgctgact gccgggatgc actggccgac ctcatggatc
      301 gttaccccgg ctgcgagatg cagatcg
//
LOCUS       DAZKHK010000542          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZKHK010000542 DAZKHK010000000
VERSION     DAZKHK010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.545"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..>327
                     /locus_tag="ACHOS5_005671"
     CDS             108..>327
                     /locus_tag="ACHOS5_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFP9780288.1"
                     /translation="MTTKNTGTIILSVAGVTLSFEPNKTAFNNLINEITMTNKVAPMS
                     TYLNRIVTPASRDALADLIARYPGCEMQI"
BASE COUNT           89 a          101 c           82 g           55 t
ORIGIN      
        1 ggcgctgatt ttgatgcggc actgcgaacc ctcacgagcc gctacaccga atgcgccgcc
       61 aggcataaca gcctggtgga aaccatacga aacacggaga aactgcaatg accacgaaga
      121 acaccggcac catcatcctg tccgttgcgg gcgtaaccct gagctttgaa ccgaacaaaa
      181 cggcatttaa caacctcatc aacgaaatca cgatgaccaa caaggtggcg ccgatgagca
      241 cctatcttaa ccgcatcgtg acacctgcca gccgcgacgc actggctgac ctgattgcgc
      301 gttatccggg ctgtgagatg cagatcg
//
LOCUS       DAZKHK010000543          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZKHK010000543 DAZKHK010000000
VERSION     DAZKHK010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.546"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>325
                     /locus_tag="ACHOS5_005672"
     CDS             <1..>325
                     /locus_tag="ACHOS5_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP9780289.1"
                     /translation="ERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVG
                     IKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKF
                     ESEVY"
BASE COUNT           82 a           73 c           93 g           77 t
ORIGIN      
        1 cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc tccatctccg
       61 gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt ggtgaagaag
      121 ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt gaaatgttcc
      181 gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg cgtggtatca
      241 aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc aagccgcaca
      301 ccaagttcga atctgaagtg tacat
//
LOCUS       DAZKHK010000544          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZKHK010000544 DAZKHK010000000
VERSION     DAZKHK010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.547"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..305)
                     /locus_tag="ACHOS5_005673"
     CDS             complement(165..305)
                     /locus_tag="ACHOS5_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9780290.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
BASE COUNT           72 a           92 c           75 g           82 t
ORIGIN      
        1 cacccgatat tctgactgtc atggatttcc cccataaaaa aaccgcagta ccggtttccc
       61 ggcctgcggt aaaatttgtg gtttgttggt gttaaaacgg tgccatccgg ctgaccaccc
      121 tcagcaaccg gtcggcgggg gatattctcc cccgccacgg tttcttactg cttacactgt
      181 aagaacgccg caatctccgc gcccgctatc cggaaccgga actcgcacag tgaactgtgg
      241 gtgatccaga taatgagcac taccgtgata caaatcacgg tggtttttaa cggtttttgc
      301 gacataaacg cttgctcctt t
//
LOCUS       DAZKHK010000545          317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZKHK010000545 DAZKHK010000000
VERSION     DAZKHK010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.548"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(54..>317)
                     /locus_tag="ACHOS5_005674"
     CDS             complement(54..>317)
                     /locus_tag="ACHOS5_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFP9780291.1"
                     /translation="NRVSIGQDVVHSIIESGIAALLIGERSPTLRSDIITRLILLVED
                     DERLVPGTVFINEEAPGRLHVTAETQDFGRINTRVNYERETGR"
BASE COUNT           58 a          100 c           76 g           83 t
ORIGIN      
        1 cgatctcttc ttctgtttcc ggcataccgc tttcacggaa tacctgatta aaatcaacgt
       61 ccggtttccc tttcatagtt caccctggtg tttatgcgtc caaaatcctg tgtctctgcc
      121 gtcacatgca gcctgcccgg tgcctcttcg ttaatgaaca ccgtccccgg aaccagccgt
      181 tcatcatcct caaccaggag gataagccgg gtaatgatgt cgctgcgcag cgtcgggctg
      241 cgttcgccga taagcagggc ggcgatcccg ctttcgataa tgctgtgcac cacgtcctgg
      301 ccgatgctga cccggtt
//
LOCUS       DAZKHK010000546          316 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZKHK010000546 DAZKHK010000000
VERSION     DAZKHK010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.549"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..>316)
                     /gene="tuf"
                     /locus_tag="ACHOS5_005675"
     CDS             complement(86..>316)
                     /gene="tuf"
                     /locus_tag="ACHOS5_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9780292.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
BASE COUNT           89 a           82 c           79 g           66 t
ORIGIN      
        1 tgacagtacg aataagatat gcagtcaaca aatgcaaaaa gggcgccgaa gcgccctttt
       61 caattcaaaa ctaattaacg tgtaattagc ccagaacttt agcaacaacg cccgcgccaa
      121 cggtacggcc gccttcacgg attgcgaaac gcagaccgtc gtccatcgcg atcgggtgga
      181 tcagggtaac aaccattttg atgttgtcgc ccggcattac catctctacg ccttccggca
      241 gttcgatggt accagtcacg tcagtagtac ggaagtagaa ctgcggacgg tagcctttga
      301 agaacggagt atgacg
//
LOCUS       DAZKHK010000547          315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZKHK010000547 DAZKHK010000000
VERSION     DAZKHK010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.550"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..141
                     /locus_tag="ACHOS5_005676"
     CDS             10..141
                     /locus_tag="ACHOS5_005676"
                     /inference="COORDINATES: protein motif:HMM:NF022230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFP9780293.1"
                     /translation="MNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            146..>315
                     /locus_tag="ACHOS5_005677"
     CDS             146..>315
                     /locus_tag="ACHOS5_005677"
                     /inference="COORDINATES: protein motif:HMM:NF016437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFP9780294.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFK"
BASE COUNT           68 a           89 c           90 g           68 t
ORIGIN      
        1 tcggacgtgg tgaacatcag cgcaggtgaa gccggaaaga ccacgaaaaa aatcaagttc
       61 atggtcacaa gcccggactt tatcagcatt gacggcgtgc cctacctgtc agcccgtgac
      121 acccgcgatc tgaaggggtg atgccatgcc ggacggtcag acttcactcc tggcaaaact
      181 gattatcatc ggtgctgcca tcgggctggg gcgtctcatg gtgagccgcg atcgcatcac
      241 agcccgtctg cttatcgggc gaatgattat gggctctgcc gtggcaccgg ttgccgccat
      301 tccgctgttt aaatt
//
LOCUS       DAZKHK010000548          313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZKHK010000548 DAZKHK010000000
VERSION     DAZKHK010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.551"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..165
                     /locus_tag="ACHOS5_005678"
     CDS             <1..165
                     /locus_tag="ACHOS5_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFP9780295.1"
                     /translation="IRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKGENNWTVE
                     KKNAVRLVRE"
     gene            172..>313
                     /locus_tag="ACHOS5_005679"
     CDS             172..>313
                     /locus_tag="ACHOS5_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFP9780296.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQL"
BASE COUNT           96 a           59 c          108 g           50 t
ORIGIN      
        1 atcagggacc aggaagagac gctggcgaaa ctccgggaaa aaacatgggg tgtgacgtat
       61 cacgagggca ggaacgggaa atttctggtg ttgccgtccg gggtgaaggg ggaaaacaac
      121 tggacggtgg agaagaagaa cgcagtaaga ctggtcaggg agtaagtgag tatgacagag
      181 ctggaaatgc acttactgaa cgccttagag caattgcagc aggactatac gcagcggctg
      241 aaagactggg agagcgcctt cacggatttg cagcatatgt tcgagctgac acgacaggag
      301 aacgggcaac tga
//
LOCUS       DAZKHK010000549          311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZKHK010000549 DAZKHK010000000
VERSION     DAZKHK010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.552"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>311
                     /locus_tag="ACHOS5_005680"
     CDS             <1..>311
                     /locus_tag="ACHOS5_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780297.1"
                     /translation="NERGAFRPGLWIFKANISLSQHSVLTDTGYGDICLGGCLIEVSG
                     DINGGLIRITTGIESPEDITRPHRTQFTGVILSKGKSAIWYRTYTDKNKPTPQDIGAL
                     PA"
BASE COUNT           90 a           90 c           71 g           60 t
ORIGIN      
        1 aatgaacgtg gcgccttcag accaggactc tggatattca aagccaatat ttcactgtca
       61 cagcacagcg tgctcactga tacgggctat ggcgatatct gccttggagg ctgccttatc
      121 gaagtatcag gcgacatcaa cggggggctc atcaggatca ctacaggcat tgaatcccct
      181 gaagacatca ccagacctca ccgcacacag ttcaccggag ttattttaag taaagggaaa
      241 agcgccatct ggtaccgcac ttacacggac aaaaacaaac ccacaccaca ggacatcggc
      301 gcactgcctg c
//
LOCUS       DAZKHK010000550          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZKHK010000550 DAZKHK010000000
VERSION     DAZKHK010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.553"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>309
                     /locus_tag="ACHOS5_005681"
     CDS             <1..>309
                     /locus_tag="ACHOS5_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP9780298.1"
                     /translation="GSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRS
                     GDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPES
                     "
BASE COUNT           77 a           71 c           82 g           79 t
ORIGIN      
        1 tggttccaaa gatattactt taaccgccgc gcatgtggct gcttttgcca gaagggcaac
       61 ggatacatat gccgatgcgg atggtggcgt tccctggaat gccgaatcag gcgcttacaa
      121 tgtcacccgc tctggcgaca gctatattct ggttaacttc tataccggag tcggaagttg
      181 ccggaccctg cagatgaagg cgcattacag aaatggtggt ctgttctacc gttcttcaag
      241 agatggctat ggttttgagg aagactgggc agaagtttat acctcgaaaa atcttccacc
      301 agaaagcta
//
LOCUS       DAZKHK010000551          306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZKHK010000551 DAZKHK010000000
VERSION     DAZKHK010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.554"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          105 a           52 c           53 g           96 t
ORIGIN      
        1 attcaagacg aaaacacgat aattttccta tagaggtaga atcctccgta gagcaggaga
       61 cacactgtat cacaaataat aagtcggctt ccggtatagt aacacatgac tatgatgccg
      121 attatatttg tggttgtggt gaaattatgt gtcctggttg cggtcatgac ctataacaaa
      181 catttttgca aactgatacc tgatcaggta caagcctgtt gattattatg ctgaatattc
      241 catgttataa atccatcact ataaacatta acaagaaaaa acatatattg tttatctgaa
      301 tattta
//
LOCUS       DAZKHK010000552          306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZKHK010000552 DAZKHK010000000
VERSION     DAZKHK010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.555"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..>306
                     /locus_tag="ACHOS5_005682"
     CDS             72..>306
                     /locus_tag="ACHOS5_005682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780299.1"
                     /translation="MSYQYSQEAKERISKLGQSEIVNFINEISPTLRRKAFGCLPKVP
                     GFRAGHPTEIKEKQKRLIGYMFQSHPSSEERKAW"
BASE COUNT          106 a           51 c           69 g           80 t
ORIGIN      
        1 tatagggcat tggtaaaaga agttaagacc aatggatttg aatgtaaccg ctttagataa
       61 ggtgccttcg aatgagttac caatatagcc aagaggcaaa ggaacggatc tctaagttgg
      121 gacaatccga aattgttaac tttatcaatg agatttctcc aactttacga cgtaaagctt
      181 ttggttgttt accaaaagta ccgggattca gggcaggaca tcccactgaa attaaagaaa
      241 aacagaaaag attgattggg tatatgttcc agtcacatcc ttcctctgag gagagaaaag
      301 catgga
//
LOCUS       DAZKHK010000553          303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZKHK010000553 DAZKHK010000000
VERSION     DAZKHK010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.556"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..128
                     /locus_tag="ACHOS5_005683"
     CDS             6..128
                     /locus_tag="ACHOS5_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9780300.1"
                     /translation="MTVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQCKQ"
     gene            185..>303
                     /locus_tag="ACHOS5_005684"
     CDS             185..>303
                     /locus_tag="ACHOS5_005684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780301.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLK"
BASE COUNT           76 a           64 c           84 g           79 t
ORIGIN      
        1 cgcttatcac cgtcacaatt tgtatgacgg ttatcttcac catctggatg ttacacggct
       61 cgttgtgtga gttccggctg aatttgtggg gagcggagtt tgcggcgttc ttacagtgta
      121 agcagtagga aaaccgcggc ggggacgaaa gtccccgtca actggttgct gaggttcagc
      181 cgatatggca cccgtttcag gtgagagaat gaacgataaa attctccggt atatgcagcg
      241 tgtggtgaga aattcccgca accctgaatt tatgaatgaa gttaaagacg cctgccttaa
      301 aaa
//
LOCUS       DAZKHK010000554          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZKHK010000554 DAZKHK010000000
VERSION     DAZKHK010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.557"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..285)
                     /locus_tag="ACHOS5_005685"
     CDS             complement(<1..285)
                     /locus_tag="ACHOS5_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP9780302.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLYALSEQQKAACVVLNPEKSHPYIEDMEMFLSPLPVVLEWHC"
BASE COUNT           75 a           93 c           62 g           71 t
ORIGIN      
        1 acaatgccac tccagcacga ccggtaatgg cgataaaaac atctccatat cctcaatgta
       61 agggtgggac ttttccggat tcagcaccac gcaggctgcc ttctgttgct cgctcagggc
      121 atataaatca tgctcaaacc atgccccctg agcatctgtc tgcaaaatca accggccatg
      181 acaggaaagg cagaaacact gccggatatt tctgctgagt gcagaatcag cactgattat
      241 gtgttcaccc ggcgtgatcc ccagccccgt ttttataccg ttcattctgc caccccctcc
      301 t
//
LOCUS       DAZKHK010000555          300 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZKHK010000555 DAZKHK010000000
VERSION     DAZKHK010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.558"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>300
                     /locus_tag="ACHOS5_005686"
     CDS             <1..>300
                     /locus_tag="ACHOS5_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780303.1"
                     /translation="KEAEAALTQAKADNKITQQEHDDLAAKNDAVTAAKADAAKAVEG
                     LPAGDAKDGLNGRLAKVDGIDVPAVDENGNGKPDAEEAAEAVNAATAKVAEAEAK"
BASE COUNT           91 a           62 c          113 g           34 t
ORIGIN      
        1 aaaaagaagc agaagcggcg ctgactcagg cgaaggctga caataagatc acccagcagg
       61 agcatgatga tcttgcggcg aagaacgatg cggtaacggc ggcgaaagca gatgcggcga
      121 aagcggtgga aggtctgccg gccggagatg cgaaggacgg tctgaacggg cgtctggcta
      181 aagttgacgg tatcgatgta ccggcagtgg atgaaaacgg caacggcaag ccggatgcag
      241 aggaagcagc agaggcggtg aatgcagcga cagccaaggt agcagaagcg gaagcgaagg
//
LOCUS       DAZKHK010000556          297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZKHK010000556 DAZKHK010000000
VERSION     DAZKHK010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.559"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..134)
                     /gene="hokA"
                     /locus_tag="ACHOS5_005687"
     CDS             complement(<1..134)
                     /gene="hokA"
                     /locus_tag="ACHOS5_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP9780304.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     A"
BASE COUNT           85 a           71 c           57 g           84 t
ORIGIN      
        1 gctaaaaacg ccgccagctc ataactctcc tgtttaatat gcaattcaca cagtgaatct
       61 cttatcatcc aggtgaaaaa taaaagcgtg aaacaaatca ctattaaaga aagtaatcta
      121 tatttctgcg gcattcccag ccccctgtgt tgatttccaa cgagtaagag gctaacctta
      181 tggtgtctat gcatagaggg gcctcacttt gatttatagt caggtggggc ttttctctgt
      241 ctgcctttcg gtgaatacct gagacaaaca gtctcaagca cccgcagcta ttctagc
//
LOCUS       DAZKHK010000557          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZKHK010000557 DAZKHK010000000
VERSION     DAZKHK010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.560"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>291
                     /locus_tag="ACHOS5_005688"
     rRNA            <1..>291
                     /locus_tag="ACHOS5_005688"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           73 a           72 c           82 g           64 t
ORIGIN      
        1 gccccggtaa acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg
       61 taagttccga cctgcacgaa tggcgtaatg atggccaggc tgtctccacc cgagactcag
      121 tgaaattgaa ctcgctgtga agatgcagtg tacccgcggc aagacggaaa gaccccgtga
      181 acctttacta tagcttgaca ctgaacattg agccttgatg tgtaggatag gtgggaggct
      241 ttgaagtgtg gacgccagtc tgcatggagc cgaccttgaa ataccaccct t
//
LOCUS       DAZKHK010000558          290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZKHK010000558 DAZKHK010000000
VERSION     DAZKHK010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.561"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..96)
                     /locus_tag="ACHOS5_005689"
     CDS             complement(<1..96)
                     /locus_tag="ACHOS5_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780305.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEP"
     gene            complement(146..>290)
                     /locus_tag="ACHOS5_005690"
     CDS             complement(146..>290)
                     /locus_tag="ACHOS5_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780306.1"
                     /translation="VSAKFARYVPVEPYTTQRDGELVTVTIKTAAATGTLNVLLRYTV
                     VGY"
BASE COUNT           71 a           75 c           76 g           68 t
ORIGIN      
        1 cggctcaaga cgcgggaaca atgtgcggct tccggtaatg gtgtcctttt tcacgggttt
       61 tgggccgata tagacaacgg caattttctc actcatataa ttccccggat aaaaagcccg
      121 catgacgcgg gccggaaggt tttaatcagt atcccaccac ggtataacgc agcagaacat
      181 tcagggtgcc ggttgcagcg gcagtcttaa tggtgacagt aaccagctcc ccgtcacgct
      241 gtgtggtgta cggctccact ggcacatatc tggcaaattt tgcagaaaca
//
LOCUS       DAZKHK010000559          290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZKHK010000559 DAZKHK010000000
VERSION     DAZKHK010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.562"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           90 a           52 c           68 g           80 t
ORIGIN      
        1 gtaattgctt aatgttatta gtgattacgg cttgaaagat aaagagtgct gtaaagtggt
       61 gatagatatc acattcatga ttaaaaaaca gaacctgtca ataaataatt catttattaa
      121 aaaggacgac ttctattatg acggaatatt atctcaatga ggaagtttgg aaatacagag
      181 ccagtttctc cggggtaacg agcaggtgta ccagtgtcag gtggcatttg tattgccggg
      241 tgagcgggtc atgatggcct tcacctacgc ccgaaccaca ccgctgaccc
//
LOCUS       DAZKHK010000560          285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZKHK010000560 DAZKHK010000000
VERSION     DAZKHK010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.563"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>285
                     /locus_tag="ACHOS5_005691"
     rRNA            <1..>285
                     /locus_tag="ACHOS5_005691"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           66 a           55 c          104 g           60 t
ORIGIN      
        1 ctgtaagcct gtgaaggtgg cctgtgaggg ttgctggagg tatcagaagt gcgaatgctg
       61 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
      121 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
      181 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
      241 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggt
//
LOCUS       DAZKHK010000561          281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZKHK010000561 DAZKHK010000000
VERSION     DAZKHK010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.564"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>281
                     /locus_tag="ACHOS5_005692"
     rRNA            <1..>281
                     /locus_tag="ACHOS5_005692"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           88 a           62 c           80 g           51 t
ORIGIN      
        1 aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg
       61 tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt
      121 aacttcggga gaaggcacgc tgatatgtag gtgaagcgac ttgctcgtgg agctgaaatc
      181 agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga
      241 aagtggacgt atacggtgtg acgcctgccc ggtgccggaa g
//
LOCUS       DAZKHK010000562          273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZKHK010000562 DAZKHK010000000
VERSION     DAZKHK010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.565"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>273
                     /locus_tag="ACHOS5_005693"
     CDS             <1..>273
                     /locus_tag="ACHOS5_005693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9780307.1"
                     /translation="RRGLPRHVKNPFLNDTNIHTKTGIRRITTGKDRLAVVNENTGEQ
                     VGHGGFFQSMEVDKTQFVKLYVDGVSAIEGLSSSGKKVFKILYLAI"
BASE COUNT           95 a           46 c           60 g           72 t
ORIGIN      
        1 aagaagaggt ctacctcgcc atgtaaaaaa cccattcctt aatgatacaa atatacatac
       61 aaaaacaggt atcagacgta tcacaacagg aaaagatcgc ttagccgtag taaatgaaaa
      121 tacaggagaa caggttgggc atggtggttt ctttcagtca atggaagtag acaagacaca
      181 gttcgtgaag ctttatgtgg atggcgtatc agctattgag ggtttatcct catccggtaa
      241 gaaagtattt aagatactgt atcttgctat tag
//
LOCUS       DAZKHK010000563          272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZKHK010000563 DAZKHK010000000
VERSION     DAZKHK010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.566"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>272
                     /locus_tag="ACHOS5_005694"
     CDS             <1..>272
                     /locus_tag="ACHOS5_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HFP9780308.1"
                     /translation="SKVLVSENNLTATTKEVAAATNIDRAMLLGAQALANAYGQKAGG
                     HFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQDHGVIAVDT"
BASE COUNT           80 a           61 c           78 g           53 t
ORIGIN      
        1 tcaaaggttc tggtatcaga gaataacctg acggcaacca cgaaagaggt cgctgctgca
       61 accaatattg accgcgccat gttactgggg gctcaggcgc tggcaaatgc ttacggtcag
      121 aaggcgggcg gtcacttcaa catggttgag aagaaaacgg atatggataa ccgtactgag
      181 atagcaatca gctggatcaa cggtctgaaa aaaatccgtt tccccgagaa gagcggcaag
      241 atgcaggatc acggcgtgat tgccgttgat ac
//
LOCUS       DAZKHK010000564          271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZKHK010000564 DAZKHK010000000
VERSION     DAZKHK010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.567"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>271)
                     /locus_tag="ACHOS5_005695"
     CDS             complement(<1..>271)
                     /locus_tag="ACHOS5_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HFP9780309.1"
                     /translation="AFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGM
                     EGKVLNLRDIEQGMEQINRLRTEPVQIEISPGDREGWSVVTLTALP"
BASE COUNT           53 a           96 c           63 g           59 t
ORIGIN      
        1 ggcaatgccg tcagtgtcac caccgaccat ccctcacggt caccgggcga tatttcaatc
       61 tgtaccggct ccgtacgcag acgattaatc tgctccatcc cctgctcaat atcccgcagg
      121 ttcagaacct tcccctccat tcccgggaaa accatcttca gggtgcgggc aggaaggtca
      181 gcgccttccg cccggatttg ctgcagcctg ccttccatga ccgttatgtg cagtacgccc
      241 cctgaaaggt cctgctctgt cagaaaggcc c
//
LOCUS       DAZKHK010000565          267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZKHK010000565 DAZKHK010000000
VERSION     DAZKHK010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.568"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>267
                     /locus_tag="ACHOS5_005696"
     CDS             <1..>267
                     /locus_tag="ACHOS5_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780310.1"
                     /translation="SVKQSGGDFTFSVIDTPSFSSVQLNGKGNNGSVTLSSEGNKLTL
                     SGGNGAGQNGVVISGVASGLEGKELSTLTNTDKEMKNAASVADLK"
BASE COUNT           83 a           42 c           74 g           68 t
ORIGIN      
        1 agcgtgaaac aaagtggtgg tgattttaca ttctctgtta ttgatactcc gtcattcagc
       61 agtgttcagc tgaatggaaa aggaaataac ggaagtgtaa cgctctcatc cgaaggtaat
      121 aagctgacat tgtcaggcgg taatggtgcc ggacaaaatg gtgttgttat cagtggtgtg
      181 gcaagtggac tggaaggtaa ggaactttcg acgttaacga atactgataa agagatgaaa
      241 aatgccgcca gtgttgcaga cctgaaa
//
LOCUS       DAZKHK010000566          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZKHK010000566 DAZKHK010000000
VERSION     DAZKHK010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.569"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>264
                     /locus_tag="ACHOS5_005697"
     CDS             <1..>264
                     /locus_tag="ACHOS5_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9780311.1"
                     /translation="SPPMIVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELP
                     DPDEEYVCGADTAEGLEHGDRSSLDVVKRSNGEQVAHWFGHLDA"
BASE COUNT           66 a           54 c           83 g           61 t
ORIGIN      
        1 tcgccaccga tgattgttta tgacattgaa cctgttacag gagcgaagac taaagctcag
       61 tctctgcgtg aaggaaataa aaacgagttg cagcggacgc tgatgaatta tctgctggta
      121 tgggaactgc cggatccgga tgaagagtat gtttgtgggg cagatactgc cgaagggctg
      181 gagcacggag accgctcatc gctggatgtt gtcaaacgca gtaatggcga gcaggtggct
      241 cactggttcg ggcatctcga tgct
//
LOCUS       DAZKHK010000567          262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZKHK010000567 DAZKHK010000000
VERSION     DAZKHK010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.570"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            83..>262
                     /locus_tag="ACHOS5_005698"
     CDS             83..>262
                     /locus_tag="ACHOS5_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9780312.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGP"
BASE COUNT           74 a           56 c           56 g           76 t
ORIGIN      
        1 tatcaaatgc gacaatcatc acattaatta atgcgtaaaa tatctactct cctgttaatt
       61 atcatcggga gttatcagtc gcatgttaaa aaatctatta aatacaaagg tcgttcaggt
      121 cgtggaacag gtcaaagact ggcgggaagc cgtggcgatt tcatgccgac cgttgattga
      181 aaacggttct atcgaaccgc gttatgttga tgcgatctat cactctcacg acactatcgg
      241 tccgtactat gtggttggcc ct
//
LOCUS       DAZKHK010000568          261 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZKHK010000568 DAZKHK010000000
VERSION     DAZKHK010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.571"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..158)
                     /locus_tag="ACHOS5_005699"
                     /pseudo
     CDS             complement(1..158)
                     /locus_tag="ACHOS5_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT           70 a           50 c           49 g           92 t
ORIGIN      
        1 atctgaaagt gattgttaat tcgggatgtc agtgagtccg ctatatccgg cagcgtctgg
       61 ttaaaaaaga cagatattgc gtccctgaac tctcgcgtac tgcttaagta gcgattattc
      121 cgggcttgtt cgatcattaa tttccacatt ctttctatcc agggaaagat catcagaagt
      181 agacctttcg tactaaataa ttcgcatttt atgttcaatg agatattcct tattatctaa
      241 agctgttttt cattgcttat a
//
LOCUS       DAZKHK010000569          255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZKHK010000569 DAZKHK010000000
VERSION     DAZKHK010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.572"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>255
                     /locus_tag="ACHOS5_005700"
     CDS             <1..>255
                     /locus_tag="ACHOS5_005700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP9780313.1"
                     /translation="TAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAM
                     NITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRR"
BASE COUNT           77 a           54 c           73 g           51 t
ORIGIN      
        1 gacggcacag gattataaat tccattttta cgcatggtgg caggatccta aatacagcgc
       61 cagagtgcct gaaagcgggc tgaagctgtc acgggaaaaa atgacgtatt tttctgcggt
      121 tgagaaggca atgaacatca cgcttaccga tgagcagaag cagtggtaca tcagtaagga
      181 aactgaacag cgtgaggaaa tgaagcagga gtttccctca acgccacagg aggcgtttct
      241 gacgtccgga cgacg
//
LOCUS       DAZKHK010000570          254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZKHK010000570 DAZKHK010000000
VERSION     DAZKHK010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.573"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>254
                     /locus_tag="ACHOS5_005701"
     CDS             <1..>254
                     /locus_tag="ACHOS5_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9780314.1"
                     /translation="MVAGAFDCADIIARVRDIKPVWALANDMNCSAGQLLASAASRRL
                     VTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHKV"
BASE COUNT           53 a           68 c           82 g           51 t
ORIGIN      
        1 atggtggcgg gggcatttga ctgcgctgac atcatcgccc gtgtgcgtga cataaaaccg
       61 gtatgggcgc ttgccaacga catgaactgc agtgcaggtc agttgcttgc cagtgccgcc
      121 tcccggcgtc tggtcacgca gaccgcccgg acaggctcca tcggcgtcat gatggctcac
      181 agtaattacg gtgctgcgct ggagaaacag ggtgtggaaa tcacgctgat ttacagcggc
      241 agccataagg tgga
//
LOCUS       DAZKHK010000571          254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZKHK010000571 DAZKHK010000000
VERSION     DAZKHK010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.574"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>254
                     /locus_tag="ACHOS5_005702"
     CDS             <1..>254
                     /locus_tag="ACHOS5_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9780315.1"
                     /translation="VAHWKAQGHERAADVPGARLGDETQLVAQYKKAVYARAKADLMG
                     EFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAG"
BASE COUNT           55 a           74 c           88 g           37 t
ORIGIN      
        1 gtggcgcact ggaaggcgca ggggcatgaa cgcgccgcag acgtaccggg ggccaggctg
       61 ggcgatgaaa cccagctggt cgcacagtac aaaaaggccg tttatgcccg tgccaaggcc
      121 gatcttatgg gcgagttcgc caccattggt cgccgtgaaa cctatccggg gcaggaaagc
      181 gaggacacac gcgccacctt actggctgaa gcctcgctgg tcatgcgtaa catgctgggg
      241 cacagacgcg cggg
//
LOCUS       DAZKHK010000572          253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZKHK010000572 DAZKHK010000000
VERSION     DAZKHK010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.575"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>253
                     /locus_tag="ACHOS5_005703"
     CDS             <1..>253
                     /locus_tag="ACHOS5_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9780316.1"
                     /translation="ACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQSA
                     LADRGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYR"
BASE COUNT           73 a           52 c           79 g           49 t
ORIGIN      
        1 gcctgcaggc ctacagagga gctggtgaag cggatgttaa ataaggggct ggaaagcctt
       61 gctgaagggg agaaaccgct gctccacagt gatcaaggat ggcattaccg gataaaaagt
      121 tatcagtccg ccctggcgga cagagggtta gtgcagagca tgtcgcgcaa gggcaactgc
      181 cttgataatg cggtaatgga gaacttcttt ggtcacctga aagaagaaat gtactatcgc
      241 cgggactaca gaa
//
LOCUS       DAZKHK010000573          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZKHK010000573 DAZKHK010000000
VERSION     DAZKHK010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.576"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           66 a           44 c           84 g           58 t
ORIGIN      
        1 ggtcttaagt acaggagtgt gaagcacctg gttggacgtg cgtagccaga atgcatattc
       61 gtgaagtcgg gtaatgagag ttgaacggtg gcagtgagaa tggagcagga aaaaatcaga
      121 tgcggaaagc ggaaatacac agcgggtgcg atgtgcttca tgctgtttgt atggctgggt
      181 ggtgtggtaa cactctgtgt acttgctgca ctgataacac aataaaagag gggagctgcc
      241 tgctccccgt gc
//
LOCUS       DAZKHK010000574          249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZKHK010000574 DAZKHK010000000
VERSION     DAZKHK010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.577"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /locus_tag="ACHOS5_005704"
     CDS             <1..231
                     /locus_tag="ACHOS5_005704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780317.1"
                     /translation="EGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPGEISLC
                     RLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
BASE COUNT           48 a           72 c           71 g           58 t
ORIGIN      
        1 gaaggcgtga ttcaggcacc agaatcaccc gacgccccgg aggcgctgcc ggggtgtcgt
       61 tccatttctg atgatgacct gtgcgcctgg tgtacccgtc tgctttaccg tcccggagaa
      121 atcagtctgt gcagactgag tacaggcaat ggcatctggc catccgtctg tgaccggaac
      181 ggttacgcgt acggctgtcc ggaatttcag ccgaatatca ttcatccctg atattaccgg
      241 gtctcacgg
//
LOCUS       DAZKHK010000575          246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZKHK010000575 DAZKHK010000000
VERSION     DAZKHK010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.578"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           60 a           74 c           70 g           42 t
ORIGIN      
        1 acgcgggacc agaacgcctg cgcggcccgc agcagtttgt cgggttcccc cctgtccatc
       61 caggagggca gtttcagcga gcgaagccgc tgcaaaaagt cattcaatga ccacctccac
      121 tgtcagggat tcgagccgtg gcacggccag atcgctgatg atgtcttcca gtgaaaactg
      181 aaccgaagcc accaccggga aggtctgatg aatttccttg cccagcgtgg aaaacgaaaa
      241 gcgcga
//
LOCUS       DAZKHK010000576          246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZKHK010000576 DAZKHK010000000
VERSION     DAZKHK010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.579"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>246
                     /locus_tag="ACHOS5_005705"
     CDS             <1..>246
                     /locus_tag="ACHOS5_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780318.1"
                     /translation="YSTKNKPDTVSQRDASTTQKGVVQLSSDTGSNDETKAATPKAVK
                     AAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGP"
BASE COUNT           82 a           54 c           83 g           27 t
ORIGIN      
        1 ttacagcaca aaaaacaaac cggatacggt cagccagcgg gatgccagca cgacgcagaa
       61 aggtgtggtg cagttaagca gtgatactgg cagtaatgac gaaacaaagg cagccacgcc
      121 gaaggccgtg aaggcggcaa tggatgtggc aaatgaagcg aaaacaaagg cagaagaggc
      181 tgcagcagga ggtggtgttc ccggtccgaa aggagagaaa ggcgaccccg gagcacaggg
      241 tccgaa
//
LOCUS       DAZKHK010000577          243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZKHK010000577 DAZKHK010000000
VERSION     DAZKHK010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.580"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>243
                     /locus_tag="ACHOS5_005706"
     CDS             <1..>243
                     /locus_tag="ACHOS5_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9780319.1"
                     /translation="QFQAIREVIRPQQEVTPEAQSQPENAEDGNGSIYPTVRFRDPNE
                     VRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMGS"
BASE COUNT           72 a           57 c           75 g           39 t
ORIGIN      
        1 acagtttcag gcaatacgag aagtcattcg cccacaacag gaagtgacgc cggaagcaca
       61 gtcacagcct gaaaatgcgg aggatggtaa cgggagcatt tacccgacgg tgcggttccg
      121 ggacccgaat gaagtccgca ttgaaattaa cgggaatggt gcgtccagac cagcggaacg
      181 cattgaaaag gtgcgcccgg acaaccgtta tttcacggat gagaaaagcg ccatggggag
      241 tga
//
LOCUS       DAZKHK010000578          243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZKHK010000578 DAZKHK010000000
VERSION     DAZKHK010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.581"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>243)
                     /locus_tag="ACHOS5_005707"
     CDS             complement(<1..>243)
                     /locus_tag="ACHOS5_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP9780320.1"
                     /translation="GVLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGY
                     TLVDYTYSWSDYLSTIDNRGWRWRSTGDLQTHRLGLS"
BASE COUNT           64 a           76 c           60 g           43 t
ORIGIN      
        1 gacagtccca gccggtgagt ctgcaggtct cccgtggaac gccaccgcca gccccggtta
       61 tcaatggtgc tgaggtagtc actccatgaa tacgtgtaat ccaccagggt atagccatac
      121 ggcagactga caccggcggc aaaattcctc gcatcatgtg acaccgaaaa gtcactgctc
      181 cgtcccccgc tgacaaacca gttgtcagcc agccccagag gattattaaa ggaaaggaca
      241 cca
//
LOCUS       DAZKHK010000579          242 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZKHK010000579 DAZKHK010000000
VERSION     DAZKHK010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.denovo.582"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>242
                     /locus_tag="ACHOS5_005708"
     CDS             <1..>242
                     /locus_tag="ACHOS5_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9780321.1"
                     /translation="ENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAMIVHAPTQKKI
                     RTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQ"
BASE COUNT           64 a           62 c           70 g           46 t
ORIGIN      
        1 gagaatgccg tggtttattc ggtcaccctg ggggcagagc tgggtgattt tgaatttaac
       61 tgggtgggac tggtcagcaa agcaaccagc acgctggcca tgattgttca cgcccccaca
      121 cagaaaaaaa tcagaaccca caacggtaac gaaggtaatg tgctggtccg ttccatgctc
      181 atggagtaca gcggtgcgaa ggaagccaca ggtatcacca ccccggcgca gacctggcag
      241 at
//
LOCUS       DAZKHK010000580        13344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.guided.131, whole genome shotgun sequence.
ACCESSION   DAZKHK010000580 DAZKHK010000000
VERSION     DAZKHK010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.guided.131"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..202
                     /gene="psd"
                     /locus_tag="ACHOS5_005709"
     CDS             <1..202
                     /gene="psd"
                     /locus_tag="ACHOS5_005709"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="catalyzes the decarboxylation of
                     phosphatidyl-L-serine to phosphatidylethanoleamine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFP9780322.1"
                     /translation="INLFAPGKVNLVEQLESLSVTKIGQPLAVSTETFVTPDAEPAPL
                     PAEEIEAEHDASPLVDDKKDQV"
     gene            224..3547
                     /gene="mscM"
                     /locus_tag="ACHOS5_005710"
     CDS             224..3547
                     /gene="mscM"
                     /locus_tag="ACHOS5_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9780323.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3686..5188)
                     /gene="yjeM"
                     /locus_tag="ACHOS5_005711"
     CDS             complement(3686..5188)
                     /gene="yjeM"
                     /locus_tag="ACHOS5_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9780324.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNTMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(5407..6384)
                     /gene="epmA"
                     /locus_tag="ACHOS5_005712"
     CDS             complement(5407..6384)
                     /gene="epmA"
                     /locus_tag="ACHOS5_005712"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9780325.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            6709..8517
                     /gene="frdA"
                     /locus_tag="ACHOS5_005713"
     CDS             6709..8517
                     /gene="frdA"
                     /locus_tag="ACHOS5_005713"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9780326.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8510..9244
                     /gene="frdB"
                     /locus_tag="ACHOS5_005714"
     CDS             8510..9244
                     /gene="frdB"
                     /locus_tag="ACHOS5_005714"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9780327.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            9255..9650
                     /gene="frdC"
                     /locus_tag="ACHOS5_005715"
     CDS             9255..9650
                     /gene="frdC"
                     /locus_tag="ACHOS5_005715"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9780328.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            9661..10020
                     /gene="frdD"
                     /locus_tag="ACHOS5_005716"
     CDS             9661..10020
                     /gene="frdD"
                     /locus_tag="ACHOS5_005716"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9780329.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            10083..11216
                     /locus_tag="ACHOS5_005717"
     CDS             10083..11216
                     /locus_tag="ACHOS5_005717"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9780330.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPVWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            11305..11838
                     /gene="blc"
                     /locus_tag="ACHOS5_005718"
     CDS             11305..11838
                     /gene="blc"
                     /locus_tag="ACHOS5_005718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9780331.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(11835..12152)
                     /gene="sugE"
                     /locus_tag="ACHOS5_005719"
     CDS             complement(11835..12152)
                     /gene="sugE"
                     /locus_tag="ACHOS5_005719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9780332.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(12335..12481)
                     /gene="ecnB"
                     /locus_tag="ACHOS5_005720"
     CDS             complement(12335..12481)
                     /gene="ecnB"
                     /locus_tag="ACHOS5_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9780333.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(12592..12717)
                     /gene="ecnA"
                     /locus_tag="ACHOS5_005721"
     CDS             complement(12592..12717)
                     /gene="ecnA"
                     /locus_tag="ACHOS5_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9780334.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(12769..13335)
                     /gene="efp"
                     /locus_tag="ACHOS5_005722"
     CDS             complement(12769..13335)
                     /gene="efp"
                     /locus_tag="ACHOS5_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9780335.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT         3364 a         3510 c         3446 g         3024 t
ORIGIN      
        1 tatcaacctg tttgcaccag gtaaagtgaa cctggttgag caactggaaa gcctgtctgt
       61 cacgaaaatt ggccagccgc tggcagtatc taccgaaacc tttgttacgc cagacgctga
      121 accagccccg cttcctgctg aagagatcga agcagaacac gacgccagcc cattggttga
      181 cgacaaaaaa gaccaggtct aatccatcaa aggaaacgct gacgtgcgcc tgattatcac
      241 ttttctgatg gcctggtgcc tcagttgggg ggcgtacgcc gcgacggccc ccgatagcaa
      301 acaaatcacc caggaactgg agcaggcaaa agcggcgaaa cccgcgcagc cggaagtcgt
      361 agaggcgctc cagtctgcct taaatgcgct tgaggaacga aaaggttccc ttgagcgcat
      421 caagcaatat caagaagtca ttgataatta tccgaaactt tccgccactc tgcgcgcgca
      481 gttaaacaac atgcgtgacg agccacgcag cgtgtcgccg ggaatgtcta acgatgcgct
      541 gaatcaggaa attctccaga tcagcagtca gttgctggat aaaagccgtc aggcccagca
      601 agagcaggag cgcgcccgcg agattgccga ttcgctgaat caactgccgc aacagcaaac
      661 cgatgcccgc cgtcagttga atgaaatcga gcgccgcctg gggacgctta ccggcaatac
      721 cccgctcaat caggcacaaa atttcgcgtt gcagtctgac tccgcacgtc tgaaagcgct
      781 cgttgatgaa ctggagctgg cgcagctatc tgccaataac cgccaggaat tagcgcgctt
      841 gcgctctgag ctggcggaaa aagagagcca gcagctggat gcatatttgc aagcactacg
      901 caaccagttg aatagtcaac gccaactgga agcggaacgg gcgctggaaa gtaccgagca
      961 actggcggaa agtagcgccg atttgccaaa agatatcgtc gcccagttta aaattaaccg
     1021 cgaactatcg gcggcgctga atcaacaggc gcagcggatg gatctcgttg cctcgcaaca
     1081 gcgtcaggct gccagccaga cgttacaggt ccggcaggcg ttgaatacgc tgcgtgaaca
     1141 gtcgcaatgg ctgggatcat ctaacctgct cggcgaagcg ttgcgggcgc aggtggcacg
     1201 gctaccggaa atgccaaaac cacaacagct tgataccgaa atggcgcagt tacgtgtgca
     1261 acggttacgt tatgaggatc tgcttaataa acagccgctg ctacggcaaa tccatcaggc
     1321 cgacggtcag ccactgaccg ctgagcaaaa ccgtattctg gaagcacagc tacgcactca
     1381 gcgtgagttg ctgaactcat tgttgcaggg tggcgacacg ctactgctgg agctgaccaa
     1441 gctgaaagtc tccaacgggc aactggagga tgcgctgaaa gaggtgaacg aagcgacgca
     1501 ccgctatctg ttctggacct ctgacgtgcg cccgatgacc atcgcctggc cgctggaaat
     1561 cgcccaggat ctgcgtcgtc tcatttcgct ggacaccttc agtcagttgg gcaaagccag
     1621 tgtgatgatg ctgaccagta aggagacgat tttgccgctg tttggcgcgt tgattctggt
     1681 cggttgcagt atttactcgc gccgctattt cacccgtttt cttgaacgtt cggcggcgaa
     1741 agtcggcaaa gtgactcagg atcacttctg gctgacgtta cgcactcttt tctggtcgat
     1801 tctcgtcgcg tcaccgctgc ctgtgctgtg gatgacactg ggttacggct tgcgcgaggc
     1861 gtggccttat ccgctggcgg tcgcgattgg tgatggcgta acagccaccg tgccgctgct
     1921 gtgggtagtg atgatttgcg ccacctttgc tcgcccgaac ggcttgttta tcgctcattt
     1981 tggctggccg cgcgaacgag tttcccgtgg gatgcgttac tacctgatga gcatcgggct
     2041 tattgtgccg ctgattatgg cgctgatgat gttcgataac ctcgacgacc gtgaattctc
     2101 cggttcgctg ggacggcttt gctttatcct catttgcggt gcgctggcgg tggtcaccct
     2161 cagcctgaaa aaggccggga tcccgctgta tctcaacaaa gagggcagcg gcgacaacat
     2221 taccaaccat atgctgtgga acatgatgat tggcgcaccg ctggttgcca ttctggcgtc
     2281 ggcggtgggt tatctggcaa cggcgcaggc gctgttagcg aggcttgaaa cctcggttgc
     2341 catctggttt ttgctactgg tggtttatca cgttatccgc cgctggatgc tgatccagcg
     2401 ccgcaggctg gcgtttgacc gggcgaagca tcgccgggca gagatgttag cgcagcgcgc
     2461 gcgtggcgaa gaggaagcgc atcatcacag tagcccggaa ggagcaattg aagtcgatga
     2521 aagcgaagtc gatctcgatg ccatcagtgc gcaatccttg cggctggtgc gctcaatttt
     2581 gatgttgatc gccctgcttt cggtcattgt gctgtggtca gaaatccatt ccgctttcgg
     2641 cttcctcgaa aatatttcgc tgtgggatgt cacttccacg gtacagggcg tagaaagcct
     2701 ggagccgatt accctcggtg cggtactgat tgccattctg gtgtttatca tcaccacgca
     2761 gctggtgcgc aacctgcccg cgctgctgga actggcgatt ctgcagcacc tggatttaac
     2821 gccgggtacg ggctacgcca tcaccaccat caccaaatat ctgctgatgc tgattggcgg
     2881 gctggtcggc ttctcgatga ttggtattga gtggtcgaaa ttgcagtggc tggtcgccgc
     2941 gctcggtgtt ggtctcggtt ttggtttgca ggaaattttc gccaacttta tctctggcct
     3001 gatcatcctg ttcgaaaaac cgattcgcat tggcgatacg gtaacgatcc gcgatcttac
     3061 cggtagtgtg acgaaaatta acacccgcgc caccaccatc agcgactggg accgcaaaga
     3121 aattatcgtg ccgaacaagg cgtttattac cgagcagttt atcaactggt cgctctctga
     3181 ctcggtcacg cgtgtggtgt tgacaattcc ggcccctgcc gatgccaaca gtgaagaagt
     3241 gacggaaatc ctgcttaccg cagcgcgtcg ctgctcgctg gtgatcgaca acccggcgcc
     3301 ggaagtcttc ctggtggatc tgcaacaggg gattcaaatt ttcgagctgc gtatttacgc
     3361 cgctgagatg ggtcaccgta tgccgctacg ccatgagatc caccaactaa ttctggcggg
     3421 cttccacgcc cacggtatcg atatgccatt cccgcccttc cagatgcgtc tggaaagcct
     3481 gaatggtaaa caaacgggga gaacattaac ttctgcgggc aaaggtcgtc aggcgggaag
     3541 tttgtaatgc atgaagctgc caaatgatgt caacgtggct catttagcag cttaacatca
     3601 tcttgccccg gagagtatca tccatacgca ttacttcctg acaactcatc gccggagaga
     3661 cgatgagttg caggatttca tatgatcagt cgagagctaa ctcaggtttg ttcgccatgc
     3721 gactgcaata gttctggtaa atcgccatcg ctaacagcga gaagaagaca gggccgccaa
     3781 tcatccacaa tgtgctgtcc cagtctccag cttcaaccac aggttgaatg atggtgaaga
     3841 cgttcgcaaa tgtcaccacc agtacgacaa ccactgttgc aatcattgcc gacatatgcg
     3901 ttttgaaaat cacaaacggt ctgtcgagat cctgacgtgc tttaaaaaat gggaaagcca
     3961 gcgcgaggaa caggtaagga agcgtcatag acacgttcgc catcagcgtc agcttgttaa
     4021 agaacgccga tgcggtaccg ccaccaaacg aaaccagcag aatgaagata gtaaccaacc
     4081 cgcactgcat ccacatggca atagacggca tccccatcgt attcaggcgc gtcatcggtt
     4141 ccggccacaa tgctttcggc gtcccctgga tgatggcttt cagcggtgaa tagcaaagtg
     4201 taaagaacgc accggtataa gcgaggaaca tcgacagtcc ggtaatacgc gcaaaccata
     4261 cgcccagcga caatgacgct tcaggtgaca aatgcagtgc gttacccagc gtcaccccta
     4321 ggctcttcat cagcacatag gtaatattgc cgaggttaac ggaaccatta cttaatacct
     4381 gctgccagtt tgtgctgacg ccccataaaa atattgccag cgaataaccg attgaaataa
     4441 caatagcggc aaaaacaata cctttggcaa agttcttttc tggattttcc gttttatcga
     4501 ccagaccacc aaccgcttca attccgccat aggcaaaaat ggcaaatacc acaaacgata
     4561 gcattgccag accggactga taacctgggt tcggtgatgc aaggaaatta atatcctgcg
     4621 cgaaatgccc accatttaat aacaaaatag taatgcttac taacagcaat actaaattca
     4681 gacacattac tgcaataccg cccaccgcag taatgcgggc aattttatta atcccttttg
     4741 aagcaacgac ggtgaccaga atcatccatg ccactgccag cagaccaacc acctgcgtag
     4801 gctccagtcc ggcaatatgc cagtgctggg tcatgtcgct accgtagagg aatgttgaga
     4861 acggtaccca aactttcgcg gaggtactca ccatccagat gatataagag gaaaaccaca
     4921 taaacgtacc aatgaaggca aaacgtggtc cgacactatt attcatccag gaatagatac
     4981 cgccttcttc tttgcgataa gcagctccca tttcagccat cattaaggcg aatggaataa
     5041 agaataacaa tgcagaaaat atataaaagg gaatcgcact ataacccatt aagtaataag
     5101 ccgatgggct attggcaaat ccaaatacag aagtaaagat catcaatatg agtcctatca
     5161 gactcatctt ttttatcgag tgaggcatga aaccatcctt catttcagcg cgtgtataaa
     5221 acgcgcccac tgaactgcac tgcccaacat tgagcgggca gcgtaaaaac caatacagaa
     5281 ttgatacctg gatgatatca aatatccagt cttaaattgt ggcctttagc ggaaaaatga
     5341 aaacatatgc tacagaatgg cgcttatcac gccattcttc gctgttaatt cagtaatttt
     5401 tcagaattat gcccggtcaa cgctaaaggc gatgacttca gccagtgtct ccgcgcccag
     5461 cgccaacatc accagacgat caacacctaa tgccacgccg gaacagtcag gcataccgac
     5521 tttcaaggct tcaatcagat tctggtcaat ggggtgctgc ggcaaaccgc gcgccgcgcg
     5581 cttacggtta tcttgttcaa agcgttgttg ctgctcacgg gcatccgtca attcatggaa
     5641 accattcgcc agctcaatac ctttataata aacctcaaag cgttcagcga cccgatgatc
     5701 ttcggtactg atttgcgcca gtgatgcctg gctggctgga aagtggtaca caaaggtcgg
     5761 tttttctttg ccaatatttg gctctacgcc aaaggtaaac agcaattgta gaagcgtgtc
     5821 gcggtcttct tcagtatcgg caacgttgct caaatccagt ttcgctgcca cttcccgcag
     5881 ttgcgttttg tcggcagata gtgggtcaat ttccagataa cgcaagaaag cttgttgata
     5941 agaaaggctt tctgctgccg gacagtccag cacctgttgc aagagatcgt ccacctcgtt
     6001 catcaaccgg tacatatcat agtgcggtcg ataccactca agcatagtga actcagggtt
     6061 gtgataacgc cccatctctt cattacggaa gctgcggcac agctggaata ccggcccaca
     6121 accggcaacc agcaggcgct tcatatggta ttccgggctg gtcattaacc agagattcat
     6181 cccctgcgaa tgcccggggc caacgaaacg tgtctcaaac gggaccaaat gaatatcggt
     6241 taccgtcgcc tggctcatac aaggcgtctc cacctccagc actccacgat cggcaaagaa
     6301 acgacggatc tccgccataa tcgccgcgcg ttttaataag ttaggaatgg atgcgctcgg
     6361 ctgccaggat gccgtttcgc tcatagttaa atctccagtt tttgacaagg gcacgaagtc
     6421 tactcgcaac gcgacggcga gacaaatttt acgcaggaat aaaacaacgg tgggcagtga
     6481 ctaaaaaaag cacgatctga tggtttagta attaaattaa tcatcttcag tgataattta
     6541 gccctcttgc gcactaaaaa aatcgatctc gtcaaatttc agacctatcc atcagactat
     6601 actgttgtac ctataaagga gcagtggaat agcgttcgca gaccgtaact ttcaggcact
     6661 taccctgaag tacggggctg tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt
     6721 caagccgatc ttgccattgt aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc
     6781 gcgcaggcaa atccaaatgc aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc
     6841 cataccgttg ctgcagaagg gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa
     6901 tatcactttc acgatacagt agcgggtggc gactggttgt gtgagcagga tgtcgtggat
     6961 tatttcgtcc accactgccc aaccgaaatg acccaactgg aactgtgggg gtgcccatgg
     7021 agccgtcgtc cggatggtag cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgt
     7081 acctggttcg ccgccgataa gaccggcttc catatgctgc acacgctgtt ccagacctct
     7141 ctgcaattcc cgcagatcca gcgttttgac gaacatttcg tgctggatat tctggttgat
     7201 gatggtcatg ttcgcggcct ggtagcaatg aacatgatgg aaggcacgct ggtgcagatc
     7261 cgtgctaacg cggtcgttat ggctaccggc ggtgcgggtc gcgtttatcg ttacaacacc
     7321 aacggcggca tcgttaccgg tgacggtatg ggtatggcgc taagccacgg cgttccgctg
     7381 cgtgacatgg aattcgttca gtatcaccca accggcctac caggttccgg tatcctgatg
     7441 accgaaggct gccgcggtga aggtggtatt ctggtcaaca aaaatggcta ccgttatctg
     7501 caagattacg gcatgggccc ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa
     7561 ctgggtccac gcgacaaagt ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc
     7621 atctccacgc cacgtggcga tgtggtttac ctcgacctgc gtcacctcgg cgagaaaaaa
     7681 ctgcatgaac gtctgccgtt catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg
     7741 gttaaagaac cgattccggt acgtccgacc gcacactaca ccatgggcgg tatcgaaacc
     7801 gatcagaact gtgaaacccg cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt
     7861 ggtctgcacg gtgcgaaccg tctgggctcc aactcgctgg cggaactggt ggtcttcggt
     7921 cgtctggccg gtgaacaagc gacagagcgt gcagcaactg ccggtaatgg caacgaagcg
     7981 gcaattgaag cgcaggcagc tggcgttgaa caacgtctga aagatctggt taaccaggat
     8041 ggcggcgaaa actgggctaa gatccgcgac gaaatgggca tggcaatgga agaaggttgc
     8101 ggtatctacc gtacgccgga actgatgcag aaaaccatcg acaagctggc agagctgcag
     8161 gaacgcttca agcgcgtgcg catcaccgac acttccagcg tgttcaacac cgacctgctc
     8221 tacaccattg agctgggcca cggtctgaac gttgctgaat gtatggcgca ctccgcaatg
     8281 gcacgtaaag agtcccgcgg cgcgcaccag cgtctggacg aaggttgcac cgagcgtgac
     8341 gacgtcaact tcctcaaaca caccctcgcc ttccgcgatg ctgatggcac gactcgcctg
     8401 gagtacagcg acgtgaagat tactacgctg ccgccagcta aacgtgttta cggtggcgaa
     8461 gcggatgcag ccgataaggc ggaagcagcc aataagaagg agaaggcgaa tggctgagat
     8521 gaaaaacctg aaaattgagg tggtgcgcta taacccggaa gtcgataccg caccgcatag
     8581 cgcattctat gaagtgcctt atgacgcaac tacctcatta ctggatgcgc tgggctacat
     8641 caaagacaac ctggcaccgg acctgagcta ccgctggtcc tgccgtatgg cgatttgtgg
     8701 ctcctgcggc atgatggtta acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga
     8761 ttacaccgac ggtatgaagg ttgaagcgtt agctaacttc ccgattgaac gcgatctggt
     8821 ggtcgatatg acccacttca tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa
     8881 ctcccgcacc gcggatcagg gtactaacat ccagaccccg gcgcagatgg cgaagtatca
     8941 ccagttctcc ggttgcatca actgtggtct gtgctacgcc gcgtgcccgc agtttggcct
     9001 gaacccagag ttcatcggtc cggctgccat tacgctggcg catcgttata acgaagatag
     9061 ccgcgaccac ggtaagaagg agcgtatggc gcagttgaac agccagaacg gcgtatggag
     9121 ctgtactttc gtgggctact gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc
     9181 cattcagcag ggcaaagtag aaagttcgaa agactttctt atcgcgaccc tgaaaccacg
     9241 ctaaggagtg caacatgacg actaaacgta aaccgtatgt acggccaatg acgtccacct
     9301 ggtggaaaaa attgccgttt tatcgcttct acatgctgcg cgaaggcacg gcggttccgg
     9361 ctgtgtggtt cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat ggcccggaag
     9421 cctgggcggg attcgtcgac tttttacaaa acccggttat cgtgatcatt aacctgatca
     9481 ctctggcggc agccctgctg cacaccaaaa cctggtttga gctggcacca aaagcggcca
     9541 atatcattgt aaaagacgaa aaaatgggac cagagccaat tatcaaaagt ctctgggcgg
     9601 taactgtggt tgccaccatc gtaatcctgt ttgttgccct gtactggtaa ggagcctgag
     9661 atgattaatc caaatccaaa gcgttctgac gaaccggtat tctggggcct cttcggggcc
     9721 ggtggtatgt ggagcgccat cattgcgccg gtgatgatcc tgctggttgg tattctgctg
     9781 ccgctagggc tgtttccggg tgatgcgctg agctacgagc gcgttctggc gttcgcgcag
     9841 agcttcattg gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg gtgtggttta
     9901 caccgtatgc accacgcgat gcacgatctg aaaatccacg tacctgcggg taaatgggtt
     9961 ttctacggtc tggctgctat cctgacagtt gtcacactga ttggtgtcgt tacaatctaa
    10021 cgcatcgcca atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg aaaccagacc
    10081 ctatgttcaa aacgacgctc tgcaccttat taattaccgc ctcttgctcc acatttgccg
    10141 cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata gagcaacaaa
    10201 agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac tttacctggg
    10261 gctatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt gagttaggtt
    10321 cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca
    10381 agttaagcga tcccacaaca aaatactggc ctgaacttac cgctaaacag tggaatggga
    10441 tcacactatt acatctcgca acctacaccg ctggcggcct gccattgcag gtgccggatg
    10501 aggtgaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct gtatgggctc
    10561 caggaacaca gcgtctgtat gctaactcca gtatcggttt gttcggcgca ctggctgtga
    10621 agccgtctgg tttgagtttt gagcaggcga tgaaaactcg tgtcttccag ccactcaaac
    10681 tcaatcatac gtggattaat gtaccgtccg cagaagaaaa gaactacgcc tggggatatc
    10741 gcgaaggtaa ggcagtacat gtttcgccag gggcgttaga tgctgaagct tatggtgtga
    10801 agtcgaccat tgaagatatg gcccgctggg tgcaaagcaa tttaaaaccc cttgatatca
    10861 atgagaaaac acttcaacaa gggatacaac tggcacaatc tcgctactgg caaaccggcg
    10921 atatgtatca gggtctgggc tgggaaatgc tggactggcc ggtaaatcct gacatcatca
    10981 ttaacggcag tgacaataaa attgcactgg cagcacgccc cgtaaaaccg attacgcccc
    11041 caactcctgc agtacgcgca tcatgggtac ataaaacggg ggcgaccggc ggatttggta
    11101 gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct aacaaaaact
    11161 accccaatcc agcgagagtc gccgccgcct ggcagattct caacgctcta cagtaaaatt
    11221 tcatcgggtc cgaattttcg gaccttttct ccgcttttcc ttgctgtcat ctacacttag
    11281 aaaaaaacca gtaaggaaac atttatgcgc ctgctccctc tcgttgccgc agcgacagct
    11341 gcatttctgg tcgttgcctg cagttcccct acgccaccgc gtggcgtgac cgtagtgaat
    11401 aatttcgacg ctaaacgtta tctcggtacc tggtatgaga ttgcccgttt tgatcaccgc
    11461 tttgaacgtg gactggaaaa agtcaccgca acatacagcc tgcgtgatga cggcggcctg
    11521 aatgtcatta ataaaggcta taaccctgac agagaaatgt ggcagcagag tgaagggaaa
    11581 gcgtacttta ccggcgcacc aactcgcgct gcgctgaaag tgtcattctt tggtcctttc
    11641 tatggcggtt ataacgttat tgcactcgat cgggaatacc gccatgcgct ggtttgcggc
    11701 ccggaccgcg actacctgtg gatactctcc cgcacgccaa ccatttctga cgaagtgaaa
    11761 caggagatgc tggcagtcgc gacccgggaa gggtttgatg tcagtaagtt tatttgggta
    11821 cagcagcccg gtagttaatg agtgctgagt ttcaaaccaa taatccccaa tacgattaac
    11881 gccagactcg ccaggcgcat cgggttagcg gactcaccga gcagcacaat gccggtgatg
    11941 gccgcgccga ctgcgccaat acccgtccac acggcataag ccgtccctac tggtaacgat
    12001 ttcatcgccc aggcaagtag cgccagactg acaatcatcg ccgtcacagt aataacactt
    12061 ggcgtcaaac gactaaagcc gtgagtatat ttcaggccaa cggcccatac cacttccagc
    12121 agaccagcaa taactaagat aatccaggac atatcaggct ccggaaaatt ggggccgtcc
    12181 ccggtgaaaa gacgcgtttg caggtcgtcc tgcaaagctg atggttgaac ggatattttg
    12241 ccaggtggca ggggaatttc aactattacc tatgtaacta tcgcagaaaa aacaaacggc
    12301 acgacacagc tgtcgtaccg ttattgctta ttgcttattg ctgcgctttc gttgctgcgc
    12361 cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg
    12421 cagttaatac tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca
    12481 taacgtcttc cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca
    12541 acatcgccaa aaatgatgga aatctgatga tttttcggag gagaagaaaa attagctggc
    12601 agcgcgggag atggagttgc cgagatgttt gatatcttcg ccgaaaccgc gagcggtgtt
    12661 acagcccgtg agcagcgcgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc
    12721 tgacctgaaa aagtgatggt gcagcctaca ggccgcacca caaccgcatt acttcacgcg
    12781 agagacgtat tcaccagagc gggtatccac tttgatgact tcgccgatct gtacgaacag
    12841 cggaacttta accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt
    12901 atcacctttc aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt
    12961 aacggagatc ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcaacca
    13021 tttagcgttg tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt
    13081 catgaagtgc cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc
    13141 agcgccttca gcagaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag
    13201 acgacgcagt ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc
    13261 ttcaaccgcg taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct
    13321 atagtacgtt gccataaggc cctc
//
LOCUS       DAZKHK010000581         4470 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.guided.245, whole genome shotgun sequence.
ACCESSION   DAZKHK010000581 DAZKHK010000000
VERSION     DAZKHK010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.guided.245"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            19..633
                     /gene="prfH"
                     /locus_tag="ACHOS5_005723"
     CDS             19..633
                     /gene="prfH"
                     /locus_tag="ACHOS5_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP9780336.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKIDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(689..2146)
                     /gene="pepD"
                     /locus_tag="ACHOS5_005724"
     CDS             complement(689..2146)
                     /gene="pepD"
                     /locus_tag="ACHOS5_005724"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9780337.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLTDENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALTATSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2407..2865
                     /gene="gpt"
                     /locus_tag="ACHOS5_005725"
     CDS             2407..2865
                     /gene="gpt"
                     /locus_tag="ACHOS5_005725"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9780338.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            2957..4201
                     /gene="frsA"
                     /locus_tag="ACHOS5_005726"
     CDS             2957..4201
                     /gene="frsA"
                     /locus_tag="ACHOS5_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9780339.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLLVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4259..>4470
                     /gene="crl"
                     /locus_tag="ACHOS5_005727"
     CDS             4259..>4470
                     /gene="crl"
                     /locus_tag="ACHOS5_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9780340.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYS"
BASE COUNT         1075 a         1105 c         1186 g         1104 t
ORIGIN      
        1 cagtggaggg aaaaaaggat gatcttgcta caactctcct ctgctcaggg gccggaagaa
       61 tgttgtctcg cagtgaaaaa agcactggac aggctgatta aagaagctac ccgacaggac
      121 gtcgcggtaa cggtgctgga aacagaaacg ggtcgctact ctgacacact gcgttcggcg
      181 ctgatttctc tggatggcga taacgcatgg gctctaagcg aaagctggtg cggcactatc
      241 cagtggattt gtccgagtcc gtatcggcct catcatgggc gcaaaaactg gtttctgggc
      301 attgggcgtt ttaccgctga tgagcaggaa caatcggatg caatccgtta tgagacgctg
      361 cgttcgtcag ggccgggcgg tcaacatgtc aataaaatcg actcggcggt acgcgccacg
      421 catctggcat ccggtattag cgtgaaggtt cagtcagagc gcagtcagca tgctaacaaa
      481 cggctggcgc gattactgat tgcctggaag ctggaacaac agcaacagga aaatagcgcg
      541 gcgctgaaat cgcagcggcg aatgttccat caccagattg aacgaggcaa cccgcgacga
      601 acgtttacag ggatggcttt tatcgaagga taatgaagga tgaaactgcc ggaaggcgtt
      661 aaacgccatc cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag
      721 tcagcagtgt ccagtaatgg ccaacgcttt cgatatgaac ttgctcatcc ggggagtgtg
      781 gaccggtgat ggttggcccg atagacacca tgtccatttc cggatacggt tttttgaaca
      841 gaccacattc gaggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct
      901 gataggtttc acgtaccaga tgcattaccg gagagttagc gtccggctgc cagccaggat
      961 atgcgccttt tgcttcggtt ttcgcacctg ccagtttacc cagcgaatcc agcatgctca
     1021 ctacgtagtc tttaccgctg tcgatcagag aacggatcag gcagtgaatt tctacgttat
     1081 tgtcggtcat ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt
     1141 cggagttgcg gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag
     1201 atgtcgccgt taatgcagct ttgtcgttcg ctacggagtc cagcagcaat gccagattct
     1261 tctctttttc tgccagttcg tttttcagga tctcctgata ggtatccacc agagatttca
     1321 gggcgtcgac tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt
     1381 tacgcagtgt gccgccgttg aaatcaataa ggcgcaggtc cagttcttct gcatgacccg
     1441 ccaggaagcg caccagcagt ttgttggcat tacccaggcc aacgtggatt tcaccgccgg
     1501 agtgaccgcc tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg
     1561 cttcacgatc taaatgcagg ttggaggtga agtcgatacc tcccgcacaa cccatgtaga
     1621 tttcaccttc ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct
     1681 gtaagccaaa agcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg
     1741 ggccgtgaac cacgttttcg tcagtcagaa ccgccagtgc agaggccata ccaatgccgt
     1801 tatccgcacc cagcgtggtg ccgcgtgctt taacccattc gccatcaata taaggctgga
     1861 taggatcttt ggtgaagtca tgcacggtgt cgttattttt ttgcggcacc atatcgaggt
     1921 gggcctgcaa gacgaccggt ttacgatttt ccatacctgc ggtagcaggt ttacgaatca
     1981 ggatattacc tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa
     2041 tgtattcagc gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa
     2101 aaatatccca cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg
     2161 tcgatcaccc gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca
     2221 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     2281 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     2341 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     2401 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     2461 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     2521 ggtctggtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     2581 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     2641 ggcgatggcg aaggcttcat cgttattgat gacctggtag ataccggtgg tactgcggtt
     2701 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggccggt
     2761 cgtccgctgg ttgatgatta tgttgttgat attccgcaaa atacctggat cgaacagccg
     2821 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     2881 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     2941 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     3001 taaacatcct gaaacctcga cgctagtccg ccgctttagt cacggcgcac aactgcttgt
     3061 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     3121 gtggatctgg cgcggcattg acccacgtga aatcctcgat gtccaggcgc gtattgtgat
     3181 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgcggcgg
     3241 caactggatt tatgagtggg ctacccaggc gatggtgtgg caacaaaaag cctgtgcgga
     3301 agaagatccg caactcagcg gtcgacactg gctgcatgcg gctacgttgt acaacattgc
     3361 tgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg
     3421 cgcctatgaa gaggccgctc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt
     3481 acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc
     3541 gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga
     3601 acgttatttt gcgccgcgcg gcattgctat gctgactatt gatatgccgt cggtgggctt
     3661 ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc
     3721 gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg
     3781 cgctaacgtt gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag tggttgcctg
     3841 tcttggtccg gtagttcata ccctgttgag tgattttaag tgccaacaac aggtgccgga
     3901 aatgtatctt gacgttctgg ccagtcgttt ggggatgcat gatgcttccg acgaagcgtt
     3961 gcgagtggag ttgaatcgct attcattaaa agtgcaagga ttgctgggac gtcgctgccc
     4021 aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg
     4081 cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta acccagtgta
     4141 tcggaatttt gacaaaggcc ttcaggaaat cacagactgg atcgaaaaac gtttgtgtta
     4201 aaaatttgct aaattttacc aatttggtaa aacagttgca tcacaacagg agattgcaat
     4261 gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc
     4321 gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg
     4381 cgtcaacgtg aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggaactgga
     4441 agcgcaggaa tcccgtttta cctacagtta
//
LOCUS       DAZKHK010000582         1465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2202H27491
            SAMN43267818-rid23077223.guided.353, whole genome shotgun sequence.
ACCESSION   DAZKHK010000582 DAZKHK010000000
VERSION     DAZKHK010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267818
            Sequence Read Archive: SRR30307243
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350561.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350561.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:01:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,729
            CDSs (total)                      :: 5,642
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 87
            rRNAs                             :: 1, 1, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 6 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 90 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 39 of 199
            Pseudo Genes (multiple problems)  :: 51 of 199
            Pseudo Genes (short protein)      :: 3 of 199
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267818-rid23077223.guided.353"
                     /serotype="O112ab:H 1"
                     /isolate="2202H27491"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-02"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHOS5_005728"
     CDS             132..1091
                     /gene="stxA2b"
                     /locus_tag="ACHOS5_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP9780341.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECQITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHOS5_005729"
     CDS             1104..1367
                     /gene="stxB2b"
                     /locus_tag="ACHOS5_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP9780342.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          401 a          296 c          353 g          415 t
ORIGIN      
        1 aagcaatcgg tcactggttc gaatccagta caacgcgcca tacttatttt ttctggctcg
       61 cttttgcggg ccttttttat atctgcgccg ggtctggtgc tgattacttc agccaaaagg
      121 aatacctgta tatgaagtgt atattgttaa aatgggtact gtgcctgtta ctgggctttt
      181 cttcggtatc ctattcccgg gaatttacga tagacttttc gacccaacaa agttatgtct
      241 cttcgttaaa tagtatacgg acagaaatat cgaccccact tgaacatata tctcagggga
      301 ccacatcggt gtctgttatt aaccacaccc caccgggcag ttattttgct gtggatatac
      361 gagggcttga tgtctatcag gcgcgttttg accatcttcg tctgattatt gagcaaaata
      421 atttatatgt ggccggattc gttaatacgg caacaaatac tttctacagg ttttcagatt
      481 ttgcacatat atcagtgccc ggtgtgacaa ctgtttccat gacaacggac agcagttata
      541 ccactctgca gcgtgtcgca gcgctggaac gttccggaat gcaaatcagt cgtcactcac
      601 tggtttcatc atatctggcg ttaatggagt ttagtggaaa tgccatgacc agagatgcat
      661 ccagagcagt tctgcgtttt gtcactgtca cagcagaagc cttacggttc aggcaaatac
      721 agagagaatt tcgtctggca ctgtctgaaa ctgctcctgt ttatacgatg acaccggaag
      781 aagtggacct cacactgaac tgggggagaa tcagcaatgt gcttccggag tttcggggag
      841 agggtggtgt cagagtgggg cgaatatcct ttaataatat atcagcgata ctgggcacag
      901 tggcggttat actgaattgc catcatcagg gggcgcgttc cgttcgcgcc gtgaatgaag
      961 agatacaacc agaatgtcag ataactggcg acaggccagt tataaggata aacaatacgt
     1021 tatgggaaag taataccgca gctgcttttc tgaatcgcag ggctcactct ttaaatacat
     1081 ccggagaata acaggagtta aatatgaaga agatatttgt agcggcttta tttgcttttg
     1141 tttctgttaa tgcaatggca gctgattgtg caaaaggtaa aattgagttc tctaagtata
     1201 atgagaatga tacattcaca gtaaaagtgg ccgggaaaga gtactggact aaccgctgga
     1261 atctgcaacc gctactgcaa agcgcacagt taacaggaat gacggtaaca atcaaatcaa
     1321 atacctgtgc gtcaggttca ggatttgctg aagtgcagtt taattaatat cagaagcatt
     1381 gctggtttcg tggtgtgcag caatgtagtt acagtgtaat caatgtcaca attcagtcag
     1441 ttgaaggttg tctgcccgac tgaga
//
